LOCUS       DAOTEZ010000001        39782 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_673_length_39782_cov_7.097943, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000001 DAOTEZ010000000
VERSION     DAOTEZ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 39782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39782
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_673_length_39782_cov_7.097943"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1843
                     /locus_tag="P5164_00005"
     CDS             <1..1843
                     /locus_tag="P5164_00005"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRY42299.1"
                     /translation="GVEVRVAPGSILRGFVRDARSRRGLPDARVRVRGETGDAGSSPG
                     AGGLFTDVEGRFEAEGLAPGKVTVTAEHADYTARTESAVLAEGGSSVEVALSRGSALG
                     GVVLSETRQPVAGASVSLEGGAPRGGAFGGGDETTTDGAGRFRFDHLSPARYTLRANV
                     PGQSSETAEAVVVEGEPVPDVTLVLSGGATLRGSVSGLPETLRGAVSISANGPRDFWA
                     AVRTGPDGRFELSGVPAGQISVRASAGDFASGTMRSASASVTVAEGQTEAEVEVVFEG
                     DGALSGRVTRGGKPVADARIVAGAARTGRSASGRTDETGAYRIEGLTPGEYEVWVQAD
                     AYGRAGNVSRTVKVDGEARLDVELPVASLFGIVVDASTKQPLAEVRVAASLEGGAESR
                     PGAATSDSNGRFFVEELEEASYTLSLRRSGYQDAKSSARAAEGGGDAGTIEMTRGDGL
                     EVRVRDGIYGIPLRSALVRVKDGRGDVGSSTWLTLDGDGKGEIAFLRPGTYTIVVAGS
                     RYAPRTFENVGVPGPPLQVTLTPGGTLELKYGPAALAKGFATLLDALGRPYAFRAYGP
                     EGRITLTAAGLATIEGLAPGSYTLAVEGMEPKAFTVTEGGKALVELP"
     gene            1914..2789
                     /locus_tag="P5164_00010"
     CDS             1914..2789
                     /locus_tag="P5164_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY42300.1"
                     /translation="MLSIADVSKTYPNGVQALRGVSLEVGTGLFGLLGPNGAGKSTLM
                     RTLATLQEPDSGQIVLDGADVLADPQGLRRRLGYLPQEFGVYPGISAEALLDHLALLK
                     GIADRRERAAQVDALLRRTNLHEHRRRAVEGFSGGMRRRFGIAQALLGDPRLVIVDEP
                     SAGLDPEERHRLHDLLVEAGESRIVLLSTHIVEDVRQLCTRMAVLSGGRILLEGKPGP
                     LVESLAGRVYRKVVTREELAACRTSLTVLSTQLRAGRTHVQVLADARPDDGFEPVAPD
                     LEDVYFTALRSGEAA"
     gene            2786..6352
                     /locus_tag="P5164_00015"
     CDS             2786..6352
                     /locus_tag="P5164_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42301.1"
                     /translation="MRANAPSAAAIAAFEWRTATRRLPFAAAAILMGLMGFALSATAF
                     GPAELRLNGAWTVTNSVGFLTLVSVFAAMILTAPVLLRDDEHRMAEIVYATAVTKPGY
                     LLGRFAGALLATATAVLAGVAGLAAGALLVPHDPARLAPFDPLPYLWAFLVLALPGVL
                     FVSAFLFAVAALTRSTPATYVAGVLLYLLYFVAALLADSPLLAGSGPQSAGAASLAAL
                     LDPFGLSAFLADTRYWTVAERNGRLVPLAGTFLGSRLLGLGASALALAFAYRRFAFRL
                     ARGAARVAADVGPERSDGPRLPVAATTTPGLAAFGSTLRVELRALFRSAPLAALFALW
                     VGYLAIETVQSFRSSELGTAVVPTAGLLVADVAIALNVLGILFLVFFAAETAWRERLA
                     RVSEVVDATPASSALFLLAKLGALSVVLVALAAAGLATALAFQAAVGRFELAPITWAS
                     LLPVAVLPLVLLALLVLLVQALVPNRHVGLVLSLAAIAFLHKGALGGPDHPLLRYAYA
                     PRLTWSDLSGFSPAAGSLAWFAAYGSAVAALLGLVTWGVWPRGTDPRPAPRLAALPRR
                     WGRGGRLGALACAAVALALGGFAFRGTNVTNRYESEEDLLAWKAAYERAYRPLEALAQ
                     PVPAHLDAAFDLEPEERRLRVRGRLRLENWTPNAIDSVWVAARRDVRVASMTLAGAIP
                     ADVDERFATRRFALGAPMAPGASLDLAFDLRIERRSPSADDDVDLVPNGTYVHGFAML
                     PSIGYRRSWEIADPAARRRAGLPERAEEDDPEAHLPESPSRMTFDVRVATPTDQLPVA
                     PGVLVSSTIAGAVRTTRFRATHPVNAFFAFASARYDVERVRHGGVDVEVYVHPGHRRN
                     VARVLEAATAALDLCVARYGPYPFPQLRIAEVPDASLRAGGFALPGVVFLAEKRGFLI
                     DTEASGRVDLLAKRVAHEVSHQWWGHQLDPASGPGATALVESLARHTELRVLKALRGA
                     AALRPVLDFELDRYLSGRTAGGEVPLVDVGRQPWLFYAKGSLAMAATSELVGEEAVDG
                     ALGALLADAKATGRAPTARDLVARLLAATPVADRPLVEEWWTRSVVHDLRVTKAAATR
                     LPGGRYRVDVVVDGRTFETTDGAEKERAFDGDVELVVRAARSERGDDEAPPLHAERHR
                     VRGPVRLSILVDGLPGEVVVDPRRLLLDRDRADDVRDVETGR"
     gene            complement(6360..6824)
                     /locus_tag="P5164_00020"
     CDS             complement(6360..6824)
                     /locus_tag="P5164_00020"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HRY42302.1"
                     /translation="MLDSLKRFFRERVLDAPPAEAGDPGRRLRLAAAALLVEVMRADP
                     DVKEEERTVVRTALQGTFGLPLAETEELVRLAEAEADAATSLYEFTSLLADGFSREER
                     KRIVELMWLVAFADAEKHPLEEHLIRRLAGLLKVPHPDFIDAKIRAREESRT"
     gene            complement(6913..7596)
                     /locus_tag="P5164_00025"
     CDS             complement(6913..7596)
                     /locus_tag="P5164_00025"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRY42303.1"
                     /translation="MTEPIAPLPEPPPRGGQLLSAVELLVGAALVLGHNVWRVLPNEV
                     PLLFVLGLASYRLRNGGFTAIGFRRPPSWARLLAIALGAAALRIALGVVVEPLAESVW
                     PPIVAPAGTEAIAANPWTALRWLGLVWTWAAFGEEIGYRGWLLGRAGDALGRSPAATA
                     FAVAASSVLFGFGHFYKGPAGVLDSGLAGLVLAGAFLLARRNLWAPILAHGFIDTFGI
                     AALFFGWAD"
     gene            complement(7630..8193)
                     /locus_tag="P5164_00030"
     CDS             complement(7630..8193)
                     /locus_tag="P5164_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42304.1"
                     /translation="MGKTTGVPSARRAGPRTFAIVCLALLSACHRQVSEPESFCLPYT
                     GTAPLAVRGLAPGQTEDDVVARLGPPDRRNAAGILESLQWQRLPDLVATVDTRTRRVT
                     EVLGNRLDAEGVEVVSWGMSEADVRAVLGKPASSKGHYRPSGSGVISIGMKRAGGTLF
                     YRRDGNDLEITLNDDSLAYIRIRPAAP"
     gene            8403..9917
                     /locus_tag="P5164_00035"
     CDS             8403..9917
                     /locus_tag="P5164_00035"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRY42305.1"
                     /translation="MLAAVAAALPLARSVAAEEYILLSSAFSSGLNGAAYRTRVRLLN
                     QGTTPVTVSARFFDQSSGTAHDAQPLVVKGRDQVSLENAIPTLFARQLGAYGPILFET
                     TGPLVIGATVSNVNACQTGAASGQWLPAIPPEQAMLAGAIGQLIVSEKRDSGDRTNIV
                     FVNPGKETATVTVKIHRGGSVAVLAVRTLEPLGPSGFRQLPLDAVEFPGLRGTTAENV
                     WIEFSSDRPVLSYATVINNMSGDPYAVLAAPDDPPAAEDPDEVTYVLPGGVPLVLVRI
                     RSGTFQMGSPAGEPGRVNMNELLHPVTLSSDYFIGKFEVTQAQWQAVMGSNPTWFPSC
                     GGDCAVDRVSWDDVAGPGGFLERLRALLGEPRFRMPTEAEWERAARGGTQTRFFFGDA
                     LDSPDSCGLPSVREYMWTCEGGSPHPVGMKKPNPFGLYDVHGNVNEWVEDWFEPYASM
                     SPQTDPKGPPTGTHKILRGGGTNASMSWSRSAFRYPYLRNAGPIWAGFRLARSR"
     gene            complement(10006..11718)
                     /locus_tag="P5164_00040"
     CDS             complement(10006..11718)
                     /locus_tag="P5164_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42306.1"
                     /translation="MAHDVRSPGSRFCRLVLVSAILSSPGASAPAPAVAGLASPVTID
                     FVLPTVANNSGVNGTAWRTRIWVFNGDPATATIRGYFGQSLPDPGQRPAAQLHVSPGR
                     TVAFDDVEVGISGPGMMSFDVDEATVTDVRRIVIRSETFNDAGLDEDGVPRRFGLGID
                     AQKVAQPYSPGATTLSADEDAFLPLPIAPGPASVASASRANLVLVSTTDAGTFDVVLM
                     SPAGEVLTDETGAECRRAVSLPPFTWHQVNDLFSWMRAAPTPNAYLAVRPTMQGAFAA
                     AIHVDNTTGDGALFAATPERAFGTDFVFPVAHAEGANGTVWRTDVSILSPRPAPADRS
                     LEIHYTPRSGLYSIAWAMHFVPAPGSVAFRDATVTMFPGSEVMTQGSPTAGSVHGRFV
                     NGPGLITTLTYNDAAAGKFGVAIPAWSPDAAFGGVERIPAVLAGIEESAATRTNLTVR
                     NMSRVPLTITLTGIDENGGWLGTGFFELAGLEERQFPSVLLDTFGLSPPATNATIVLD
                     HVPPPGSGPDSGRFIAYATSINNGTGDSAYLPARPLPAGAVAGAAAADVAFVDDGTRA
                     VRPR"
     gene            11968..12675
                     /locus_tag="P5164_00045"
     CDS             11968..12675
                     /locus_tag="P5164_00045"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HRY42307.1"
                     /translation="MLRAYEATLEGWIRALDLRDRETEGHSRRVVGLTEKLAEELGVC
                     GEKLVHIRRGALLHDVGKMALPDSVLLKPGELTEEEQAIMHRHPEHAWEMLKGVDFLR
                     PALDIPYAHHERWNGTGYPRGLSGAEIPLAARLFAVVDVWDALRYDRAYRAGWPAERV
                     RNHLREGAGTQFDPAVVPAFLRILERDGEPVFADPANGRRGGGEATAPLGAARRARRP
                     GTARLAPVPSSAMRASS"
     gene            complement(13974..15668)
                     /locus_tag="P5164_00050"
     CDS             complement(13974..15668)
                     /locus_tag="P5164_00050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRY42308.1"
                     /translation="MRTLALLAAFAASAASAAEPPATPPRAAASVEVTLFNLDVVVTD
                     PSGARVRGLKAEDFEVRHDGKAVPVTNFSEIRAGEAAPGREPEPGAAAPAAAARPPRR
                     IVLFFDRLYVPDAIRRAELFDALSRLLEVTITEGDEAMVVTWNRSIRTVLPFTGDVEL
                     LAATLRGIERQSGRIAPERSDQDLLRASDEWFSSLAADPRIGVDFGGFMPSAELAAQQ
                     AFFEMKAKTSALKGLAATLGGMDGRKVLVLVSHRFSRYAGLEFLLKDRTGPEALGDPK
                     TRQFDSKRLLEEVARAANANGVTLHGVFPAELDAAMPSADRSRLSAPGLSDQPLGGKE
                     RIVLDNELEALSFVAEKTGGVAAVGAGNVDRFVDRVASDLESWYSIGYPAPPGAGRAV
                     PVSVKVKGRKLDVRVRSSLVEKTTAEQMADRVLAHLFKPDGRARIPISASASPAPSSG
                     GKYRIRVEVQVPISSLVLLPTAKGVQGAFSVLVASVAAAGDFSEVTRRSQPFEIPAAE
                     LETAKAGHYTYELDVEAAGPDARICVGVWDEKGSDAGFAVVSTAAPAAPPAAPPAG"
     gene            15736..17013
                     /locus_tag="P5164_00055"
     CDS             15736..17013
                     /locus_tag="P5164_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42309.1"
                     /translation="MLSFASLFVGLVFGIVPVKVVATAPVVRVELYLDGERVGDLREP
                     FEARLDLGCEPAPHELVAVGYDARGRQVDRIRQWINRPRPPAEATLLLEPGSGGKERI
                     ARLSWRCLTEENPRSITVTLDDVPLAAEDPSAIALPPHDPTKVHALKAVLDFGRGVVA
                     TAEAIFGGPRRSEAFTEMTAVAVETPDGAALPPAADLTGWFESRGAPLEVAAVEEGPR
                     EVVVLSEGSALAQLRKLFRYRTTAFEPSLPEDAVLRYVWPVAQTAAQSEMRTNVYPIT
                     RPLGPGDGSLSWIAARRLDWPAWRPGEQRISDALAVAALAAADGERRRAVVLLLGPEA
                     ADASLLSPAEATEFLGHLRVPLVVWAVGAKPPSNATPWGSATPITTARQFDAAVASLF
                     EKLGRQRIVWVEGTHLPQDVQAGALATGVRMAR"
     gene            complement(17191..>17747)
                     /locus_tag="P5164_00060"
     CDS             complement(17191..>17747)
                     /locus_tag="P5164_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42310.1"
                     /translation="DARGLRVATVAEAIAWLAETLGRTPGLSIGDFRVVEEGGRLVAA
                     AAFWDQRPVRRLRIAGLDAPNRWKARAWSALARLSGGVGPDTRGGPLAVCYLRFPSAE
                     AGHEAALARLVRAELADARFRRRFAALFAGFHENDPLRSCLDGLVRFSVPSTVLLTIP
                     KSDPALTARTYDPRRPYFVDLSLV"
     assembly_gap    17748..17757
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17758..18301)
                     /locus_tag="P5164_00065"
     CDS             complement(<17758..18301)
                     /locus_tag="P5164_00065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY42311.1"
                     /translation="MIEVREAGPEDEPALAALERKALQRAPVVFLHEHVPGLGAFLAL
                     QGDAHAVWVAEEDGVIVGCIADSTRRALFDGTERRVYYLGGLRVSPDASPRTGFALMT
                     RARRRLEDGGHGLGLAVVLSGNAPGLAFVESFTRRRHEVVRAGEVSAIVVPPLPVYRT
                     SGRGLPRAAREDDLPALASLL"
     gene            complement(18298..19659)
                     /locus_tag="P5164_00070"
     CDS             complement(18298..19659)
                     /locus_tag="P5164_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42312.1"
                     /translation="MDRLYDLPGLGMLAKVARVKRDLAGIRRRLRATAPEVKELRAAA
                     LRAVCLHAFRRSPFYRELWSAASLREDDLSALRDPSLLPVVSKEQLRSAWPDGLCADP
                     AALRRAREIRTTGSTGTPVRVALDREKVLFTIALFSRPSMEMHLGLRLERGLHVMVRS
                     PQAIEGQALREFPAAEEVFLDALSPVEVLIEELNRRRPQYVLSYPGIVGAIATEARRR
                     GVAPWQPEQFVFSGESLNPRVVASIRRTFPRAGLAQAYCATEVGGIAVQCHEGKGLHT
                     LEYQTVVEVLDDEDRPAAPGVTGRIVVTDLRNRVTPVIRYAGLADLGAWAEERCTCPL
                     AAYPLLARLEGRAAEAIRVPGGPVVHQFALTSALERLEEVAAFQVRHETATRVRLLVV
                     PEPSAGEARREALRAEAADFLRPLLPGITIEVELVPALGREPGSHKTPAVVSLVPRDG
                     GLE"
     gene            complement(20294..20950)
                     /locus_tag="P5164_00075"
                     /pseudo
     CDS             complement(20294..20950)
                     /locus_tag="P5164_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012162347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(20960..22150)
                     /gene="devC"
                     /locus_tag="P5164_00080"
     CDS             complement(20960..22150)
                     /gene="devC"
                     /locus_tag="P5164_00080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease DevC"
                     /protein_id="HRY42313.1"
                     /translation="MSGLLAAARDRLFPLGVPLAWRQLAAERKRFLAAVAGVTFAVTL
                     MLFQMGIYVAIFEKVVYPHRALRGDLVLTSRDYLNLYSNGLFTRRRLEQALAVEGVES
                     VAPLWVGSATMRNPVTRQNMRICVFGILPSRNPFALPEVERSLGVLSTTEDVLFDRRG
                     LKEYGPIARLLAERGPVETEIGDRRVVIRGLFSMGGTISSGGHVLAGEEAYFRLVPAH
                     PRNMINVGLVRLRPGVDAALAAERLRAALPRDVTVFTHEAFVAEEKRYWNERSPLAFV
                     FLGTMLVAMTVGAVIVYQVLYTDVSDHIHEYATLKAIGAGDRFFLSLVLQQAVILMAC
                     GFVPGTLATALLSAVARSKAVMPAYLTAGNVSLVLVLAFLMCALAGVLALRRLRNADP
                     ADVF"
     gene            complement(22147..23220)
                     /locus_tag="P5164_00085"
     CDS             complement(22147..23220)
                     /locus_tag="P5164_00085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRY42314.1"
                     /translation="MNVSRESTSGRDRISRGAARCRCVAAKAAVAGLLVVLAAACGKG
                     RAREGAEAAPADAPARAVHCLGRVVPGEKVLALAAPPQSVVKELLVRRNDRVRKGQAL
                     VVFRSFDAADAAARQAEAEVRVAEVLLAQARGEEKLSAIAALEAGLERARREVEKAKA
                     DRARAERLRAEGLLPESDREAAELDLRRAETTEEEASRRLVALRTSRPTEIAVAERRL
                     ASAKAALERARAEADLCVLRAPIDGVVLDVAAWPGETVGTRGVLDLGDTDAMRVEAEV
                     YVTDVARVRVGSPVRVRGDGVPGELSGRVTEVVGQVGGNALREADPNSWADRRVVKVL
                     VRLDEGRTLASLSNAQVDVTIGP"
     gene            complement(23429..23881)
                     /locus_tag="P5164_00090"
                     /pseudo
     CDS             complement(23429..23881)
                     /locus_tag="P5164_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238499.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
     gene            24016..24855
                     /locus_tag="P5164_00095"
     CDS             24016..24855
                     /locus_tag="P5164_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HRY42315.1"
                     /translation="MVEILGVRFDELTEAQTVRRILDAIDERRGGVVVTPNLDHLRRC
                     SIDATYRTLVAKADLVVADGMPIVWASRVAGQPLPERVTGADLVASLSAGAAERGRSV
                     FLLGGDPGTAEGAARVLSEGTPRLKVAGTWFPPRGFERDPVESRRLEAAVSAAAPDVV
                     FVGLGSPKQELLIDRIRSLLPAAWWLGVGISFSLLTGEEKRAPAWMRRSGFEWLHRLA
                     CEPRRLYRRYLVDGLPFALRLAAWSVRRRFRRRGPAHDGTAAPGGGRDGPVNRCVEGS
                     RPA"
     gene            24852..26519
                     /locus_tag="P5164_00100"
     CDS             24852..26519
                     /locus_tag="P5164_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42316.1"
                     /translation="MTRAEVPPWCLAGMALVPYATVPLSLEAGLRTQAGLLVIALVVV
                     AWSGRQSLRDAFGRLPPAVRVGLCLYAGAALWGAVVGAFAAHPSRSVVSQLVSLWLLP
                     AAALAFGKHPDLDGEALVRALGWAATAAAAFHAVGLAVPRLAGDLSGHFGFELRNSIG
                     IVGLAPLLWLVGLALVCARTQPASALVPVVAAGVLCFCAQSRGGWLISAAGSLGILLL
                     VQRRSILVRLLVGALAVAPLVATAAAWASARAEPVPVVDGRAPTRLESPDAAGAFSEA
                     FGEGAIEGAALEVSAVLRGVRGRRLWVWADFSDEEGRALGRQSAEVIGTGDWRGWSEL
                     LAAPPGARSFRAGLRLDPGQGAWEAEGVSATVVKSRLVGLGRQLRRRASSLGRVLADP
                     GADSAIAYRLAEARLVLDGWRRAGTARRIAGFGLGATIELPHATFDDEGRRAVAPRSS
                     YLHNFYLFLLYKLGLGGAAALAGLALITGWTVRSALSLRARPESSWLLAGVGVALLAY
                     LVWSLTSPEIYDFRIAPLWGAMLAACGRAAERGPSVGPAGPGDGRPA"
     gene            26516..27880
                     /locus_tag="P5164_00105"
     CDS             26516..27880
                     /locus_tag="P5164_00105"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HRY42317.1"
                     /translation="MSARAIRSLLRSAQRAPVVDALSATGFTLVGKSVGFLVPLFVAA
                     QFGVTAATDAFFFVLGTVLAVTGVLAALLELVVPSVAELRGRDEELAAFMNGLLGTAT
                     LAVALGFAAFLAVLGPLLPVVTRFPAGDLALVARLLLETSPLVLLVLWGSVLSGYLNA
                     HRVFGVPALTPAVRGAVAIACVLLFGDTLGVHALPLGYVAGETARLVVLGVAVRRRAP
                     FPLRPELRRDSRLAGFYRALGYPAVATVLTLVNPFVDSIAASWLAPGSVTVLRYAESL
                     YMIPFSLFTGGLVPVFLAHWSDRARDGELAALRRDVTRSARLVLWLTVAVATVLALLS
                     RPIASLLLAHGSFDAAKVPQVALVWACCLAAFPAQAVAQLHVKALFVLRRTRIVMVCA
                     LCVAPLNLVLDLLLMRVLGVAGIALSTAGTALVTLAIVSSGLRTAFVERARGAGGGDV
                     PPSP"
     gene            27861..29027
                     /locus_tag="P5164_00110"
     CDS             27861..29027
                     /locus_tag="P5164_00110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY42318.1"
                     /translation="MSRPPPDAEQTPAVVHLLNGDLFGGVERVVATLCRNRRRVRSEA
                     VCLLDGEMRRVSGVACELVPMAGRLDVGVAVRVARLIRRRRGRLLVTHTLRSSLVGAL
                     AARFANVPHVVTVHSPAARETGARLRDARNGALQRLLLPWADAIVAVSAALRDELVSA
                     GIPASRIEVVLNGVETGLCDGGDASAFRTCLPGGRRAAPLVGTHALLRPRKGLEDLLR
                     AAPAILRAHPSCTFVVVGGPERPDYPSALRQLCLDLGIADSVVFTGHRDDIADVLAAL
                     DVFVLPSLFGEGLPLALLEAMAAGRAAVATDTEGIREVVESGRTGLLVPPRDPASLAG
                     AVNALLSDRSRRSELGSAAREAARRRFGVGRMTAEAEAAYLRVLAARTSRWSSA"
     gene            complement(28979..30163)
                     /locus_tag="P5164_00115"
     CDS             complement(28979..30163)
                     /locus_tag="P5164_00115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY42319.1"
                     /translation="MREPRVLHVIANLERGGAQELLCTLAECRPAPAEILVCTLADGP
                     LRARLEAGGALCRVVPGPRVTFARPVRWVSELRKVLGALAACVEEWGADIVQTHLLPV
                     LDPAILSLHRTPRAPGVIWTFHGTDVLPLRPGRSLPLRRLACRARYRHAARRVDAIVA
                     VSEAVRESVARQLGAVAGGRTRVIENAPSPRKHEPAGPTAFLRQALGLDADARVVLSV
                     GRLSAEKGCRRLVEAMPAVAARVPAAVAMLAGDGPERRDLEALARHTGVADRVRFLGD
                     RDDVAGLLAVADVVCLPSEREGMSLSILEAMAAGRAVVALDIRANRSLVEDGVTGVLV
                     SPLERGALARCVADLLSDPVRADALGRAARARVLSRHDPWRQAGLYAELHRDVLAART
                     RR"
     gene            complement(30156..31481)
                     /locus_tag="P5164_00120"
     CDS             complement(30156..31481)
                     /locus_tag="P5164_00120"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRY42320.1"
                     /translation="MRRGLARLVAGVSVIWLLAAALIPRDSALRPAYLFNTYGLFPVL
                     TATVLVGQAASSRVQRTFRLSLPDAALSGFLAFAAISLALRSVAGAASPRDAADLWRA
                     FVVPAAAFWAVRAARLREEDFDGWVAPLSALCAVELAVGLLAWFSPASLPAFWPGAVE
                     ETGGLRVVGTLTQPDLYSAVLVFAATLLARTGRTAASPAMRWAASTGFALAFVGVFLS
                     FSRASWLGGALALALLVAACGRSVALPCLAIALAVVGLSQVPSREAKVPATESPLVAA
                     APASRDATPAREPYAVTRLKMSGTIADRIVLASAGLRMFLQKPLLGWGFGTYDRHARA
                     HVTDVGPFVASEWDRRWAASHCTHVSVLAEMGLLGYVLFVAPAAALATATVRRRRRDR
                     ALTRLWAVAAFLAVVSVLVDLRYVTTALGVAGLVLGLIAVRVEDDRPDA"
     gene            31570..32400
                     /locus_tag="P5164_00125"
     CDS             31570..32400
                     /locus_tag="P5164_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRY42321.1"
                     /translation="MSGDTSSEAQRREHYEIERELAARLRAAPREERRRLYAALYDEL
                     YRRVPHHPQRAAPSSAAETARAVRREIAVLRPFLSPASTFLELGPGACALSLAVSPLV
                     ARVWAVDVTDALVPPRPHPPGFAFVRSDGCSVPVPPGSVNVAYSHQVLEHLHPEDALA
                     QLVSTRDALAAGGVYVCVTPNRLSGPHDVSRGFDPVATGFHLKEYTAAELRTLLLAAG
                     FARVEALVGARGRFVRLPAAAVVPCERALQAIPTAFARPLARSAPFRALLGLRLVAFR
                     "
     gene            complement(32732..36436)
                     /gene="metH"
                     /locus_tag="P5164_00130"
     CDS             complement(32732..36436)
                     /gene="metH"
                     /locus_tag="P5164_00130"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HRY42322.1"
                     /translation="MRPAAPAPPALAEALAHRIVVLDGGMGTMIQPYRLDEAAYRGAR
                     FADHPRELKGCADVLPLTRPHVVAEIHRAYLDAGADVIETCTFNAQAISLADYGLEAH
                     ARELNAAAARVAREAVRGFEAANPGRHAWVAGSIGPTNRTLSLAVDVNDPGKRTNVFA
                     DFVTAYADQVRGLLDGGVDLLLVETVFDTLVGKAALVAVEEVFAERGTAVPLMLSVTI
                     TDRSGRTLSGQLVEAFWNSVSHAPLLSVGINCALGAKEMRPYVEELSRIAPVLLSAYP
                     NAGLPNAFGGFDETPESMAKDLRAFAEAGWVNFVGGCCGTNPNHIRAIAEAVRGLPPR
                     AVPAVERLTRLSGLEPLTIRPDSNFIVVGERTNVTGSPKFAKLVAANDYEGALGVARQ
                     QVEGGANVLDVCMDEGMLDSVSAMRRFLNLLSADPDIAKLPVMIDSSRWEVIEAGLQS
                     LQGKSIVNSISLKDGEAAFRERARTVRRHGAAAVVMAFDEAGQADTAERKVEVCRRAY
                     RILVEEEGFPPEDVIFDPNVLTVGTGIEEHADYALAYFEATRRIKAELPRAKVSGGVS
                     NVSFSFRGNNPVREAMHAAFLYHAIAAGMDMGIVNAGQLAVYEEVPKELLGMVEDVLL
                     NRRPDATERLVAYAETLKAKEGGAAHAAVHAEAWRSASVEERLAHALVKGNADHVEAD
                     TLEALEKLGAPLAVIEGPLMAGMNVVGDLFGSGKMFLPQVVKSARVMKKSVAVLTPFL
                     EARKAAGTMKAAGKVLLATVKGDVHDIGKNIVGVVLACNGYEVIDLGVMVAADRIAKA
                     ARETGANLVGLSGLITPSLDEMVHTVRELSREGIAVSILIGGATTSPAHTAVRIAPAS
                     PQAVVHVADASRAVGVAGNLLSDELRPAFLAKNAALQEELRRDHAGRQTRPLLPLSSA
                     RARRRALDWSAADLPVPSFTGVRVEEPALTDLVPYVDWSPFFHAWELRGRYPQILDDA
                     TIGEKARELFADAQALLAEIVDGKLLSARGAWGFFPANSVGDDVELYADAAGEHLLAT
                     LHTLRQQQEKSGDAPYEALADFVAPRTSGRRDHVGLFAVTAGHGLPALCERFEKDHDD
                     YRSILAKALADRLAEAFAERLHERAREEWGFGERLSHDDLIRERYRGIRPAPGYPACP
                     DHTEKRTLFALLDAPARTGITLTESLAMLPTASVSGLYLAHPEAHYFAVGRLGRDQVA
                     DYARRKGMPLAEAERWLAPNLGYEAEGTGA"
     gene            complement(36433..38349)
                     /locus_tag="P5164_00135"
     CDS             complement(36433..38349)
                     /locus_tag="P5164_00135"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HRY42323.1"
                     /translation="MKHDIRERLKTEVLLADGAMGTLLVSRGAAPESPRSPLSIAQTG
                     LVREVYDDYVEAGTQILKTNTWDANRVKLAKFDWADSLEKINRTAVRLAHEAAAGEYV
                     LVAGDVGPLGQLVKPYGPLTKAMVRELFSEQIRILLEEKVDLLLLETFSSTLEAIEAI
                     RAARDLSKEIPILASMTFLSDGKTTFGTEVAGAFARLEEAGADILGLNCTIGPQETLE
                     VFQRVISQTSLPVSAMPNAGYPWNVSGRTVYPATPDYFREVARDFVRAGAAIVGGCCG
                     TTPAHVAAMSKEVSGKRRASVERVARSFVAAPAAPEPEALETSVLKRKLTDPKALVVT
                     VEIEPPKGTDCSVALEGAQLLRSYGVDAVNVTDNPMARLRMSSIAVAHMIRHETGVDT
                     VFHFSPRDRNVLGIQSDLLGAAGLGIKALLVVGGDPLKIGDYPQGRAVGEVDTLGLLR
                     IVKGLNGGVDLAGAPIGTSTAFAIACAANPAAHDLDVEISKLRAKIEAGATFAQTQPV
                     YDVAALDRFFARPEARAIPVLVGLIPPKSLKQALYFANEVPGMVVPEAILVRMRKAAE
                     KGPEFEAEEGLAIGVELAAAIVERARGIHLMPMARYEVVRKVLESLPGRDAEARPSAA
                     ARDAAAKDAPGGVN"
     regulatory      complement(38410..38532)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            38682..>39782
                     /gene="purD"
                     /locus_tag="P5164_00140"
     CDS             38682..>39782
                     /gene="purD"
                     /locus_tag="P5164_00140"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRY42324.1"
                     /translation="MRVLVVGSGGREHALVWKLAQSPLVTEIFCAPGNPGIAQIATCL
                     PIGVTDIVELADFAEKMKVDLTVVGPELPLTLGLVDELTKRRLLAFGPNKHAAELEGS
                     KVFSKAFMRKYEIPTAEAEVCTTRAEAEKAVKAFGLPCVFKADGLAAGKGVVIARDEA
                     EKEKALKLFFGERVFGSAGDRVLVERFVTGDEVSCMVLCDGERAIPLATAKDYKRVFD
                     GDRGPNTGGMGGHSPSVFVDATTAREILTDIVHPTLRGMAEEGRPFRGVLYVGLMLEN
                     GMKPYVLEYNVRFGDPETQCVLVRLEDDLLPYLLGSARGKFEENAKPVWKKEAGAAVV
                     LSAEGYPGPFERGKPIEGIEDATSEEGVFVFHA"
BASE COUNT         5190 a        14308 c        14877 g         5397 t
ORIGIN      
        1 cggcgtggag gtccgcgtgg cgcccgggag catcctccgg ggattcgtga gagacgctcg
       61 ctctcgccgg ggcctcccgg acgcacgggt tcgcgtccgc ggggagacgg gggacgcggg
      121 ctcgtcgccg ggagcgggcg gcctcttcac cgacgtggag ggccggttcg aggcggaggg
      181 cctcgccccc gggaaggtga cggtcacggc cgagcacgcc gactacacgg cgcgaaccga
      241 gagcgccgtc ctcgcggagg gggggagcag cgtcgaggtc gccctctcgc gcgggagcgc
      301 gctcggaggc gtcgtccttt ccgagacgcg ccagccggtg gcgggggcct cggtctcgct
      361 cgaagggggc gccccgaggg gaggggcgtt cggcggaggc gacgagacga cgaccgacgg
      421 cgcgggccgc ttccggttcg accacctctc cccggcccgg tacacgctcc gcgcgaacgt
      481 gccggggcag tcgagcgaga cggccgaggc ggtcgtcgtc gaaggagagc cggtgcccga
      541 cgtgacgctc gtcctctcgg gcggcgccac gcttcgcggc tcggtctccg ggctgccgga
      601 gacgctccgc ggggccgtca gcatctcggc gaacgggccg cgcgacttct gggccgcggt
      661 gcggaccggc cccgacggac ggttcgagct ttccggcgtc ccggccggac agatctcggt
      721 ccgcgcctcc gcgggagact tcgcgtccgg gacgatgagg tcggccagtg cctcggtgac
      781 cgtcgcggag gggcagaccg aggccgaggt ggaggtcgtc ttcgaggggg acggcgccct
      841 ctcggggcgc gtgacgcgcg gcggaaaacc cgtggccgac gcccgcatcg tcgccggcgc
      901 cgcgcggacg ggacggagcg catcgggccg gaccgacgag acaggcgcct accggatcga
      961 ggggctcacg ccgggcgagt acgaggtctg ggtgcaggcc gatgcatatg gccgggccgg
     1021 gaacgtctcg cgcacggtga aggtcgacgg cgaggcgcgc ctcgacgtgg agctcccggt
     1081 cgcgagcctc ttcggcatcg tcgtcgacgc gtcgacgaag cagccgctcg ccgaggtccg
     1141 ggtcgccgcc tccctcgagg gaggtgcgga aagccgtccc ggtgcggcga cgagcgactc
     1201 gaacgggagg ttcttcgtcg aggagctcga ggaggcgagc tacacgctga gcctcagaag
     1261 gagcggctac caggacgcga agagcagcgc gcgcgccgcc gaggggggcg gcgacgccgg
     1321 gacgatcgag atgacgcgcg gcgacgggct cgaggttcgc gtccgcgacg ggatctacgg
     1381 catcccgctc cggagcgcgc tcgtccgggt gaaggacggc cgcggcgacg tcggctcctc
     1441 gacgtggctc acgctcgacg gcgacggaaa gggggagatc gcgttcctcc ggcccgggac
     1501 ctacacgatc gtcgtcgcgg ggtcgcgcta cgcgccccgg acgttcgaga acgtcggcgt
     1561 cccgggcccg ccgctccagg tgaccctcac gccgggcggg acgctggagc tgaagtacgg
     1621 cccggccgcg ctcgcgaagg gcttcgcgac cctcctcgac gcgctcggcc ggccctacgc
     1681 cttccgggcg tacggtccgg aggggcgcat cacgctcacg gcggccggcc tcgccacgat
     1741 cgagggcctc gccccgggca gctacacgct cgcagtcgag ggaatggagc cgaaggcctt
     1801 cacggtcacg gagggcggga aggcgctcgt cgagctgcca tagggacgcg ccgggaaccg
     1861 gacccccgtg gtcttcgttc cttcctctgc catccgggcg ggggaggtga gccgtgctct
     1921 cgatcgccga cgtgtcgaag acgtacccga acggggtgca ggcgctccgg ggcgtgagcc
     1981 tcgaggtcgg taccggcctc ttcggcctcc tcgggccgaa cggcgccggg aagtcgacgc
     2041 tcatgcggac cctcgcgacg ctccaggagc ccgactccgg gcagatcgtc ctcgacggcg
     2101 ccgacgtcct cgccgacccg caggggctgc ggcgccggct cggctacctg ccgcaggagt
     2161 tcggcgtcta ccccgggatc tcggccgagg cgctcctcga ccacctcgcg ctcctgaagg
     2221 ggatcgcgga ccgcagggag cgggcggccc aggtcgacgc gctcctccgc cggacgaacc
     2281 tccacgagca ccggcggcgc gcggtcgaag gcttctcggg cgggatgcgc cgccgcttcg
     2341 gaatcgccca ggcgcttctc ggcgacccgc ggctcgtcat cgtcgacgag ccctcggcgg
     2401 gtctcgaccc cgaggagcgg caccgcctcc acgacctcct cgtcgaggcg ggcgagagcc
     2461 ggatcgtcct gctctcgacg cacatcgtgg aggacgtgag gcagctctgc acgaggatgg
     2521 cggtcctctc cgggggccgc atcctcctcg agggcaagcc cgggccgctc gtcgagtcgc
     2581 tcgcgggacg ggtctaccgg aaggtcgtca cgcgcgagga gctcgcggcc tgccgaacct
     2641 ccctgacggt cctctcgacg cagctccggg ccggccggac gcacgtccag gtcctcgccg
     2701 acgcccggcc cgacgacggg ttcgagcccg tcgcgcccga cctcgaggac gtctacttca
     2761 cggccctccg ctcgggcgag gccgcgtgag ggcgaacgcc ccgtcggccg cggcgatcgc
     2821 cgccttcgag tggcgcaccg cgacgaggcg cctcccgttc gccgccgcgg cgatcctgat
     2881 gggcctcatg ggcttcgccc tctcggcgac ggctttcggc ccggcggagc tccgtctgaa
     2941 cggggcctgg accgtcacga actccgtcgg cttcctgacc ctcgtctccg tcttcgccgc
     3001 gatgatcctg acggcgccgg tcctgctgcg cgacgacgag caccgaatgg ccgagatcgt
     3061 ctacgcgacg gcggtcacga agcccggcta cctcctcggg cggttcgcgg gcgcgctcct
     3121 cgccacggcc acggccgtcc tcgccggcgt cgccggtctc gccgccgggg cgctcctcgt
     3181 cccgcacgat cccgcgcgcc tcgccccgtt cgacccgctt ccctacctct gggccttcct
     3241 cgtcctcgcg ctccccgggg tcctcttcgt ctcggccttc ctcttcgccg tcgcggcgct
     3301 cacccggagc acgccggcga cctacgtcgc gggcgtcctc ctctacctcc tctacttcgt
     3361 ggccgccctc ctcgcggact cgccgctcct cgcggggagc gggccgcaga gcgcgggagc
     3421 cgcgtcgctc gccgcgctcc tcgacccgtt cggactttcc gcctttctcg ccgacacgcg
     3481 ctactggacg gtcgccgagc gaaacgggag gctcgtcccg ctcgcgggga cgttcctcgg
     3541 aagccgcctc ctcggactcg gcgcctccgc gctcgcgctc gccttcgcgt accgccgctt
     3601 cgcgttccgc ctcgcgcgcg gcgcggcccg cgtcgcggcg gacgtcggtc cggagcggtc
     3661 ggacgggccg cggctgcccg tcgccgcgac gacgacgccc ggcctcgcgg cgttcggctc
     3721 gaccctccgc gtcgagctcc gcgcgctctt ccggagcgcg ccgctcgcag ccctcttcgc
     3781 gctctgggtc ggctacctcg cgatcgagac ggtccagagc ttccgatcga gcgagctcgg
     3841 gacggccgtc gtcccgaccg cggggctcct cgtcgccgac gtcgcgatcg ccctgaacgt
     3901 cctcggcatc ctcttcctcg tcttcttcgc cgccgagacg gcctggcgcg aacggctcgc
     3961 ccgcgtctcg gaggtcgtcg acgcgacgcc cgcgtcgagc gccctcttcc tcctcgcgaa
     4021 gctcggggcg ctctcggtcg tcctcgtcgc gctcgccgcg gccggcctcg cgaccgcgct
     4081 cgccttccag gccgccgtcg gccggttcga gctcgcgccg atcacgtggg cctcgctcct
     4141 ccccgtcgcg gtccttccgc tcgtcctcct cgcgctcctc gtcctcctcg tccaggcgct
     4201 cgtcccgaac cggcacgtcg ggctcgtcct ctcgctcgcc gcgatcgcgt tccttcacaa
     4261 gggcgccctc ggcggcccgg accacccgct cctgcgatac gcctacgcgc cgcgcctgac
     4321 ctggtccgac ctctcggggt tctcccccgc ggcggggtcg ctcgcctggt tcgcggccta
     4381 cgggagcgcc gtcgccgcgc tcctgggcct cgtgacctgg ggcgtctggc cgcgagggac
     4441 cgacccgcgc cccgcgccgc gactcgcggc tctcccgcgg aggtggggcc ggggcgggcg
     4501 gctcggcgcg ctcgcctgcg ccgcggtcgc cctcgccctc ggcggcttcg cgttccgcgg
     4561 aaccaacgtg acgaaccgct acgagtccga ggaggacctc ctcgcctgga aggcggcgta
     4621 cgagcgggcc taccgccctc tcgaggccct cgcccagccc gtgccggcgc acctcgacgc
     4681 ggcgttcgac ctcgagccgg aggagcgccg ccttcgggtc cggggccgcc tgcggctcga
     4741 gaactggacg ccgaacgcga tcgactcggt ctgggtcgct gcccggcgcg acgtccgggt
     4801 cgcgtcgatg accctcgcgg gagcgatccc cgcggacgtg gacgaacgct tcgcgacgcg
     4861 ccggttcgcg ctcggcgcgc cgatggcgcc gggcgcctcg ctcgacctgg cgttcgacct
     4921 ccggatcgag cgccgctcgc cctccgcgga cgacgacgtc gacctcgtcc cgaacggtac
     4981 gtacgtccac ggattcgcga tgctcccgtc gatcggctac cggcggagct gggagatcgc
     5041 ggacccggct gcgcggcggc gcgcgggcct cccggagcgg gccgaggagg acgacccgga
     5101 ggcccacctc cccgagagcc cgagccggat gaccttcgac gtccgcgtcg cgacgccgac
     5161 agaccagctc ccggtcgcgc ccggcgtcct cgtctcctcg acgatcgccg gtgccgtccg
     5221 gacgacgcgg ttccgcgcga cgcaccccgt gaacgcgttc ttcgcgttcg cgtcggcccg
     5281 ctacgacgtg gagcgcgtcc gccacggcgg cgtcgacgtg gaggtctacg tccaccccgg
     5341 gcaccggcga aacgtggcgc gggttctcga ggcggccacc gccgcgctcg acctctgcgt
     5401 cgcgcgctac gggccgtacc ccttcccgca gctgcggatc gccgaggtgc ccgacgcgag
     5461 cctccgggcg ggaggcttcg ccctcccggg cgtcgtcttc ctcgccgaga agcgcggctt
     5521 cctgatcgac acggaggcgt cggggcgcgt cgacctcctg gcgaagcggg tcgcgcacga
     5581 ggtctcgcac cagtggtggg ggcaccagct cgacccggcg agcggcccgg gcgcgacggc
     5641 cctcgtcgag tcgctcgccc gccacaccga gctgcgcgtc ctgaaggcgc tccgcggggc
     5701 ggccgcgctc cgtcccgtcc tcgacttcga gctggaccgc tacctctcgg gccggacggc
     5761 ggggggcgag gtgccgctcg tcgacgtcgg gcggcagccg tggctcttct acgcgaaggg
     5821 ctccctcgcg atggccgcga cctccgagct cgtcggcgag gaggccgtcg acggcgccct
     5881 cggcgccctc ctcgcggacg cgaaggcgac gggccgtgcg ccgaccgcgc gggacctggt
     5941 cgcgcgcctc ctcgccgcga cccccgtcgc ggaccgtccg ctcgtcgagg agtggtggac
     6001 ccgcagcgtc gtccacgacc tccgcgtcac gaaggcggcc gccacgcgtc tgccgggcgg
     6061 ccggtaccgc gtcgacgtcg tcgtcgacgg ccggacgttc gaaacgacgg acggcgcgga
     6121 gaaggagcgc gcgttcgacg gcgacgtgga gctcgtcgtc cgcgccgccc ggagcgaacg
     6181 cggagacgac gaggcgcctc cgctccatgc cgagcggcac agggtccgcg gcccggtccg
     6241 tctctcgatc ctcgtcgacg gcctgccggg cgaggtcgtc gtcgacccgc gccgcctgct
     6301 cctcgaccgg gacagggccg acgacgtccg cgacgtcgag acgggacgct gaggggccgc
     6361 tacgtccggc tttcctcccg cgcccggatc ttcgcgtcga tgaagtcggg gtgggggacc
     6421 ttgaggagcc ccgcgaggcg tcgaatcagg tgctcctcga gcgggtgctt ctcggcgtcg
     6481 gcgaacgcga cgagccacat cagctcgacg attcgcttcc tctcctcgcg ggagaagccg
     6541 tcggcgagga ggctcgtgaa ctcgtagagc gaggtggcgg cgtccgcttc ggcctcggcg
     6601 aggcgaacga gctcctcggt ctcggcgagc ggcaggccga aggtcccctg gagcgccgtg
     6661 cggacgaccg tccgctcctc ctccttcacg tcggggtcgg cgcgcatcac ctcgacgagg
     6721 agcgccgcgg ccgcgaggcg aagtcgccgg cccggatcgc ccgcctccgc gggcggcgcg
     6781 tcgaggaccc gttcccggaa gaaccgcttg agcgaatcga gcatgcccgg agtctctcgt
     6841 ccctcgccgg cctcgtccac ttcggcctcc gtcggcccgt cctcgaacgc ctcgggtcca
     6901 cggaccgccg gctcagtcgg cccagccgaa gaagagcgcg gcgattccga acgtgtcgat
     6961 gaagccgtgc gcgaggatcg gcgcccagag gttccggcgg gcgaggagga aggcgccggc
     7021 gaggacgagg ccggccagcc ccgagtcgag gacgcccgcg ggccccttgt agaagtgccc
     7081 gaagccgaag aggacggacg aggcggccac ggcgaaggcc gtcgcggcgg gagaacgtcc
     7141 gagggcgtcg cccgcccgcc cgaggagcca gccccggtac ccgatctcct cgccgaacgc
     7201 ggcccaggtc cagacgaggc cgagccagcg aagggccgtc cacgggttcg cggcgatcgc
     7261 ctccgtgccg gcgggcgcga cgatcggcgg ccagacgctc tccgcgagcg gttcgacgac
     7321 gaccccgagc gcgatgcgga gcgccgctgc gccgagggcg atcgcgagga gccgcgccca
     7381 ggaggggggc ctccggaagc cgatcgccgt gaagccgccg tttcgcagcc ggtacgaggc
     7441 gaggccgagg acgaagagga ggggcacctc gttcgggagg actctccaga cgttgtggcc
     7501 gaggacgaga gcggcgccga cgaggagctc cacggcggag aggagctgcc ctccgcgcgg
     7561 ggggggctcc gggagaggag cgatgggttc cgtcatcgag tcccgtaacg agcgacggcg
     7621 gctccgggtt cagggggccg cgggccgtat ccgaatgtaa gcgaggctgt cgtcgttcag
     7681 ggtgatctcg aggtcgttcc cgtcgcgccg gtagaagagc gtcccgccgg cccgcttcat
     7741 cccgatcgag atcactcccg agcccgaggg gcggtagtgc cccttgctcg acgccggctt
     7801 cccgaggacc gcgcggacgt cggcttcgct cattccccac gacacgacct cgaccccttc
     7861 ggcgtcgagc cggttcccga ggacctccgt cacccgccgc gtccgcgtgt cgaccgtcgc
     7921 gaccaggtca gggaggcgtt gccattggag cgactcgaga atgccggccg cgttccggcg
     7981 gtcgggcggt ccgagccggg cgacgacgtc gtcctcggtc tgtccgggcg cgaggccccg
     8041 gaccgcgaga ggagccgtcc cggtgtaggg gaggcagaag gactcgggct ccgagacctg
     8101 tcgatggcac gcggagagga gcgcgagaca gacgatcgcg aaggtacgag ggccggcgcg
     8161 ccgcgccgat gggactcccg tcgtcttccc catggctcag tctggcggga tgcctgttcc
     8221 gcggcaagcg cgacgacgcc gttggcgacc agcccctgca tgtcttcggg gtaacctctt
     8281 ccccgagtga tcgatgagga gaccgtcgag ggcggcggga gccggagccg gcctctcggc
     8341 gcgagacggc gatcgcggaa ggcaggagga atatgagacg aaacggaggc gcaaggaccc
     8401 tcgtgctcgc ggcggtcgcc gccgcgctgc cgctggcccg gtcggtcgcg gcggaggagt
     8461 acatcctcct ctcgtcggcc ttttccagcg gtctgaacgg agccgcgtac cggacgcgcg
     8521 tgaggcttct gaaccagggg acgacccccg tcacggtgag cgcgaggttc ttcgaccagt
     8581 cgagcggaac cgctcacgac gcccagccgc tggtcgtgaa ggggcgcgat caggtctccc
     8641 tggagaacgc catccccacg ctcttcgcgc ggcagctcgg cgcgtacggt ccgatcctct
     8701 tcgagaccac gggtccgctc gtcatcggag cgaccgtgag caacgtgaac gcctgccaga
     8761 cgggggcggc gtcggggcag tggctgccgg cgatccctcc cgagcaggcg atgctcgccg
     8821 gcgcgatcgg acagctgatc gtcagcgaga aacgggactc gggcgatcgg acgaacatcg
     8881 tcttcgtgaa tccggggaaa gagaccgcga cggtgacggt gaagattcac cgcggcggca
     8941 gcgtggccgt cctcgcggtc cggaccctcg aacctctggg gccgagcggg ttccgccagc
     9001 ttccgctcga cgccgtcgag tttcccgggc tccgcgggac gacggccgag aacgtctgga
     9061 tcgagttctc gagcgaccgg cccgtcctct cctacgcgac cgtgatcaac aacatgtcgg
     9121 gtgaccccta cgcggtcctg gccgcgccgg acgacccgcc cgcggccgag gacccggacg
     9181 aggtcaccta cgtccttccc ggcggagtcc ccctggtcct ggtgcggatt cggtcgggga
     9241 cgttccagat ggggtcgccg gccggcgagc cgggtcgcgt gaacatgaac gagctcctgc
     9301 atcccgtcac gctttcctcg gattacttca tcgggaaatt cgaggtgacc caggctcagt
     9361 ggcaggcggt catgggctcg aacccgacgt ggttcccgag ctgcggcggc gattgcgccg
     9421 tcgaccgcgt gtcgtgggac gacgtcgccg gaccgggcgg attcctggag aggctgcggg
     9481 cgctcctcgg cgaaccgagg tttcggatgc cgacggaggc cgagtgggag cgggccgccc
     9541 gaggagggac gcagacccgg ttcttcttcg gggacgccct cgattccccc gacagctgcg
     9601 ggttgccgtc cgtccgcgag tacatgtgga cctgcgaggg cggatccccg caccccgtcg
     9661 ggatgaagaa gcccaacccg ttcggcctct acgacgtcca cgggaacgtc aacgagtggg
     9721 tggaggactg gttcgagccc tacgcctcga tgtcgccgca gaccgacccg aagggcccgc
     9781 cgaccggaac ccacaagatc ctcaggggcg gcgggacgaa cgcctcgatg tcctggtcgc
     9841 gttcggcctt ccgctatccc tatctcagga acgccggccc gatctgggcg ggattccgcc
     9901 tcgcgcggtc caggtagcga tcggaggccg ctggaggggc gagcgttcgc tcattcgaac
     9961 cccctctcgg aagaggcgcg acccgcctga cggggatggc ctccctcagc ggggccggac
    10021 ggcccgcgtt ccgtcgtcga cgaaagcgac gtccgccgcc gcggctcccg cgacggcccc
    10081 cgccggaagc gggcgcgccg gcaggtacgc gctgtctccc gtgccgttgt tgatcgaggt
    10141 cgcgtatgcg atgaaccggc ccgagtccgg accggagccc ggagggggta cgtggtcgag
    10201 gacgatcgtg gcgttcgtcg cgggcgggct cagcccgaac gtgtcgagga ggacgctcgg
    10261 gaactgtcgc tcctccaatc cggcgagctc gaagaaacca gtcccgagcc agccgccgtt
    10321 ctcgtcgatt ccggtcagcg tgatcgtgag gggcacgcgg ctcatgttcc ggacggtcag
    10381 gttcgtcctc gtggcggcgg actcctcgat ccccgcgagg acagccggaa tccgttcgac
    10441 gccgccgaag gccgcgtcgg ggctccacgc ggggatcgcg actccgaact ttcctgccgc
    10501 ggcgtcgttg tacgtgagcg tcgtgatgag gccggggccg ttcacgaacc ttccgtgcac
    10561 gctcccggcc gttggcgacc cctgggtcat cacttcgctt cccgggaaca tcgtgaccgt
    10621 cgcatcccgg aatgccacgc tccccggtgc ggggacgaag tgcatcgccc aggcgatcga
    10681 gtagagcccg cttcgcggcg tgtagtgaat ctcgagggac cggtcggccg gagccggcct
    10741 cgggctcagg atcgagacgt cggttctcca gaccgtcccg ttcgcaccct ccgcatgagc
    10801 gaccgggaag acgaagtcgg ttccgaacgc ccgttccggg gtggcggcga agagagcgcc
    10861 gtcgcccgtc gtgttgtcga cgtggattgc agccgcgaag gcgccctgca tcgtcggacg
    10921 gaccgcgagg taggcgttcg gcgtcggcgc cgccctcatc cacgagaaga ggtcgttcac
    10981 ctggtgccag gtgaaggggg gcagggatac cgcccggcgg cattcggcac cggtctcgtc
    11041 cgtcagcacc tcgcccgcgg ggctcatgag gacgacgtcg aacgttcctg catccgtcgt
    11101 cgacacgaga acgaggttgg cccgcgacgc gctcgccacg gacgcgggac cgggagcgat
    11161 ggggagcggc aggaaggcgt cctcgtccgc cgacaacgtg gtcgcgcccg gcgagtaggg
    11221 ctgcgccacc ttctgcgcgt cgatcccgag gccgaaccgt cgagggacgc cgtcctcgtc
    11281 gagcccggca tcgttgaacg tctcgctccg gatgacgatg cggcggacgt cggtcaccgt
    11341 cgcttcgtcg acgtcgaagg acatcattcc cggtccggag atgccgacct cgacgtcgtc
    11401 gaacgcgacg gtccgaccgg gcgagacgtg cagctgcgcg gccgggcgct gcccgggatc
    11461 cggaaggctc tggccgaagt agcctcggat ggtcgccgtt gcggggtcgc cgttgaagac
    11521 ccagatgcgc gtgcgccagg cggtcccgtt cacgccggag ttgttcgcga cggtcgggag
    11581 gacgaagtcg atcgtcacgg gcgaagccag gccggcgacg gcgggcgccg gcgccgaggc
    11641 acccggcgag gaaaggatcg ccgatacgag gacgagccgg cagaagcgcg acccgggcga
    11701 gcgaacgtcg tgagccaacg agggagcctc gcggagaagt ataggtgccg agggcgcagc
    11761 ctccgccccg caatccgccg cgctcgagag cgttccttcc cggagtcgcg ctccacgaag
    11821 acgacggggc cggacggcgc gtccttgacc ggcccgcggg ccttcgctac ggtcgcgtgg
    11881 gggagggacg gagtggcgga tcgtcggagg ccggtcgagc gggcacggcg cccggaggcg
    11941 tcgggggcgc gggattctgc cgacgaggtg ctccgcgcct acgaggcgac gctggaaggg
    12001 tggatccgcg cgctcgacct ccgcgaccgg gaaaccgagg ggcacagccg ccgcgtcgtg
    12061 gggctcacgg agaagctcgc ggaggagctc ggcgtctgcg gcgagaagct cgtccacatc
    12121 cgccgcggcg ccctcctcca cgacgtcggg aagatggcgc ttcccgactc cgtcctcctg
    12181 aagccgggcg agctgaccga ggaggagcag gcgatcatgc accgtcatcc ggagcacgcc
    12241 tgggagatgc tgaagggggt cgacttcctc cgtcccgcgc tcgacattcc ctacgctcac
    12301 cacgagcggt ggaacggcac aggctacccg cgcggactct cgggtgccga gatcccgctc
    12361 gctgcgcggc tcttcgccgt cgtcgacgtc tgggatgccc tgcgctacga ccgcgcctat
    12421 cgggcgggct ggccggcgga gagggtcagg aatcacctgc gcgagggggc cgggacgcag
    12481 ttcgaccctg ccgtggttcc cgcgttcctg cggatcctcg agcgcgatgg cgagcccgtc
    12541 ttcgccgacc cggcgaacgg gcgacggggg ggcggagagg cgacggcgcc gctcggtgcg
    12601 gcccgccgcg cccggaggcc ggggaccgcg cgcctcgctc cggtgccttc gtcggcgatg
    12661 cgggcgagct cgtagagcga cgtcgcggcg tccgcctcgg cgagcaagag gccgaacgtc
    12721 ccctggagcg ccgtgcggac gacggtccgc tcctcgtccc tcgtctcggg gccggcgcgc
    12781 atcgcctcga cgaggagcgc cgccgccgcg aggcggaggc gccggcccgg gtcgccagga
    12841 gtcgcgggtg tcgcgtcgag gacgcgctcc agggagaacc gctcgagaga gtcgagcagg
    12901 gcccgggccg cgagattcgc cgcatggacc gcccctgcga acctccgcca tcctcgcaag
    12961 acgactcctt cccgccgcgg ccttcgtcgc gacgcggcgg ctcgatcccc ggccgccggc
    13021 ccatccggag ctcctcgagg tcgtcttcct cgagccgtcc ggcggcgagc ggaagctcga
    13081 gcacggggcc attctcggag cggaccactg caccttcgac gggacgacgc tctcctgggc
    13141 gctccgcccc gagggggctc cgccgtggga aggaccgacg accctcacgt acaggctccg
    13201 ctggaaggcc tggggggcga tgacgcgcgt ctgggcgccg cggcccgcca cccggccgag
    13261 ggccgcgagc tcgccgggcg accgcctctc ggtgcggtgg aacgagaccc gcgaggcgct
    13321 gaggcgagcg ggtcccaacc ctcttcgccg ctacgtcctc gacctgaacg tcgcggcccc
    13381 gggccgggag gggccgatcg agacgctcga ctgcggcctc gcgggggacg actcctggag
    13441 cttcgccggc aatctcacgt ccgtgacgct cgagcgggcg ctggcacgag gcgagggggt
    13501 cgccctgtcg ttccttttcg atcgggaaga cggggggatt ccatccggcg tcgcgcacca
    13561 cctcccggcg gcgctcctcg gcgagggcgc gccgcgcgcg ccgggggccg gcaccgccgg
    13621 gcgccggttg cgagacgcga aggcggtgat cgtcgaccgg caggagccgc cgaacctcgt
    13681 cctccgcgct gacgcggccg agctggggcc gccggaggcc gcgctcgtcg gcctcctctt
    13741 cggcgaacgg ggctccgtgc cgctcgccga ggccccctgg ttccgggcgg ccgggatgga
    13801 ggtctcgctc gcggagcccg gagtgcccga gggggacctc gaggcggtcc tcgggctctc
    13861 taccggcgag gaggagggat cgcccgagca ggctcgtcgc cgggacactc ctcgtgccct
    13921 gcggttcgag gttcctccga tgaacggggg gatcccgcgg gcgagcccgg cgcctatccg
    13981 gcgggcggcg ccgccggcgg ggcggccggt gcggcggtgc tcacgacggc gaagccggcg
    14041 tcgctcccct tctcgtccca gaccccgacg cagatccgcg cgtccgggcc ggccgcctcc
    14101 acgtcgagct cgtacgtgta gtggcccgcc ttcgcggtct cgagctccgc cgcgggaatc
    14161 tcgaacggct ggctccggcg ggtgacctcg gagaagtcgc cggcggcggc cacggaggcg
    14221 acgaggacgg agaaggcgcc ctggaccccc ttcgcggtcg ggaggaggac gagggaggag
    14281 atggggacct gcacctcgac gcggattcga tactttccgc ccgacgaggg cgccggcgag
    14341 gccgacgcgg agatcgggat tcgcgcccgg ccgtccggct tgaagaggtg ggcgaggacc
    14401 cggtcggcca tctgctcggc cgtcgtcttc tcgacgagcg agctccggac ccgaacgtcg
    14461 agcttccgtc ccttcacctt caccgagacc ggcaccgccc gccccgcacc cggcggcgcg
    14521 gggtagccga tcgagtacca cgactcgagg tcggaggcga cgcggtcgac gaagcggtcg
    14581 acgttccccg caccgacggc cgcgacgcct ccggtcttct cggcgacgaa ggagagcgct
    14641 tcgagctcgt tgtcgaggac gatccgctcc ttcccgccga gcggctggtc cgagagtccg
    14701 ggcgcggaga ggcgagaccg gtcggccgag ggcatcgccg cgtcgagctc ggcggggaag
    14761 accccgtgga gcgtgacgcc gttcgcgttc gccgcgcgcg ccacctcctc gaggagccgc
    14821 ttcgagtcga actgacgggt cttcgggtcg ccgagagcct cgggtcccgt ccggtccttg
    14881 agcaggaact cgaggccggc gtagcgcgag aagcggtggg agacgaggac gaggaccttc
    14941 cgcccgtcca tcccgccgag cgtcgcggcg agccccttca acgcggacgt cttcgccttc
    15001 atctcgaaga acgcctgctg cgcggcgagc tcggccgagg gcatgaagcc gccgaagtcg
    15061 accccgatcc gcgggtcggc agccagggag ctgaaccact cgtccgaggc gcgcaggagg
    15121 tcctggtcgg accgctccgg ggcgatgcgg ccgctctgcc gttcgatccc ccggagggtc
    15181 gccgcgagga gctcgacgtc gccggtgaag gggaggacgg tgcggatcga gcggttccag
    15241 gtgacgacca tcgcctcgtc cccctccgtg atcgtcacct cgaggaggcg cgagagggcg
    15301 tcgaagagct cggcgcggcg gatggcgtcg gggacgtaga gccggtcgaa gaagaggacg
    15361 atccggcgag gcgggcgtgc ggcagcggcg ggtgcggcgg cgccgggttc cggctcccga
    15421 cccggagcgg cctctcccgc ccggatctcg ctgaagttcg tgaccgggac ggcctttccg
    15481 tcgtggcgga cctcgaagtc ctccgccttc agcccgcgga cgcgggctcc ggacgggtcg
    15541 gtgacgacga cgtcgaggtt gaagagcgtg acctcgaccg aggccgcggc gcgcgggggc
    15601 gtcgccggcg gctccgcggc ggaagccgcc gaggcggcga aggcggcgag gagggcgagt
    15661 gtgcgcatct ggttccgtcg aagcacgcac gccgcgtgcc ggacgtgccg tattctggag
    15721 catacgcagg cggccatgct ctcgttcgcg tcgctgttcg taggactcgt cttcgggatc
    15781 gtcccggtca aggtcgtcgc gaccgccccc gtcgtccgcg tcgagctcta tctcgacggg
    15841 gagcgcgtcg gcgacctccg ggagccgttc gaggcgcgcc tcgacctcgg ctgcgagccg
    15901 gccccgcacg agctcgtcgc cgtcggctac gacgctcgcg gccggcaggt cgaccgaatc
    15961 cggcagtgga tcaaccggcc gcggcccccc gccgaggcga cgctcctcct cgagccggga
    16021 tcgggcggca aggagaggat cgcgaggctc tcctggcgtt gcctcacgga ggagaacccg
    16081 cgctcgataa cggtgacgct ggacgacgtc cccctcgccg ccgaggaccc gtcggcgatc
    16141 gccctcccgc cccacgaccc gacgaaggtc cacgcgttga aggccgtcct cgacttcggc
    16201 cgcggcgtcg tcgcgaccgc cgaggcgatc ttcggcggac cgcgccggag cgaggcgttc
    16261 accgagatga cggccgtcgc cgtggagacg ccggacgggg cggctctccc tccggcggcc
    16321 gacctgaccg gctggttcga gagccgcggg gcgccgctcg aggtcgccgc ggtggaggag
    16381 gggccgcgcg aggtcgtcgt cctctccgag gggagcgcgc tcgcgcagct ccgaaagctc
    16441 ttccggtacc ggaccaccgc cttcgagccg agcctcccag aggacgccgt cctccgctac
    16501 gtctggcccg tcgcgcagac ggcggcgcag tcggagatga ggacgaacgt ctacccgatc
    16561 acgcgcccgc tcggcccggg tgacgggagc ctgagctgga tcgcggcgcg ccggctcgac
    16621 tggcccgcgt ggcgtcccgg cgagcagagg atctcggacg ccctcgcggt ggcggccctc
    16681 gccgccgcgg atggggaacg gaggcgggcg gtcgttctcc tcctcgggcc cgaggcggcg
    16741 gacgcgagcc tcctctctcc ggccgaagcg acggagttcc tggggcacct ccgtgtcccg
    16801 ctcgtcgtct gggcggtcgg cgcgaagccg ccgtcgaacg cgacgccctg ggggagcgcg
    16861 acgccgatca ccacggcacg gcagttcgac gcggctgtgg cgtcgctgtt cgagaagctc
    16921 ggccgccaga ggatcgtctg ggtcgaaggg acccaccttc cccaggacgt gcaggcggga
    16981 gcgctggcga cgggcgtccg gatggcgcgc tgaagcgcgc ccgcgcgact cgcatcgagg
    17041 ccatcgggcc acggcgcggc gactcgacga gctcgacgga agcggggttg acgagcgagg
    17101 ccgcgaagac cgacgggtgc ttcgtgagcg tgcacgcttc ctggaggtcg gaccgcgcct
    17161 ccggccggcc gcgccgcgca ggagccccgt tcagacgagc gagaggtcga cgaaatacgg
    17221 ccggcggggg tcgtacgtgc gcgccgtcag cgccgggtcg gacttcggga tcgtcaggag
    17281 gaccgtcgac gggaccgaga accgcacgag gccgtcgagg caggagcgaa gcgggtcgtt
    17341 ctcgtggaag ccggcgaaga gggccgcgaa ccggcggcgg aagcgggcgt cggcgagctc
    17401 ggcgcggacg aggcgggcga gcgccgcctc gtgcccggcc tcggcggaag ggaagcgaag
    17461 gtagcagacc gcgagcggcc cgccccgcgt gtcggggccg accccgcccg agaggcgcgc
    17521 gagcgccgac caggcccgag ccttccagcg gttcggagcg tcgaggccgg cgatgcggag
    17581 gcgccgcacc ggccgctggt cccagaaggc cgcggccgcg acgagccggc cgccctcctc
    17641 gacgacccgg aagtcgccga tcgaaaggcc cggcgtccgg ccgagcgtct cggcgagcca
    17701 ggcgatggct tcggcgacgg tcgcgacccg caggccgcgt gcgtcgannn nnnnnnnaga
    17761 ggagggaggc gagggccgga aggtcgtcct cccgtgcggc ccgcgggagg ccccgccccg
    17821 aggtcctgta gaccggcagc ggcggaacga cgatcgccga gacctcgccg gcccggacca
    17881 cctcgtgccg ccgtcgggtg aaggactcga cgaacgcgag ccccggcgcg ttcccggaga
    17941 ggacgaccgc cagccccagg ccgtgccccc cgtcctcgag ccgccggcgc gcgcgcgtca
    18001 tcagggcgaa tcccgtccgg ggggaagcgt ccggggagac gcggaggccg ccgaggtagt
    18061 agacgcggcg ctcggtgccg tcgaagaggg cgcgccgggt ggagtcggcg atgcacccca
    18121 cgatgactcc gtcctcctcc gcgacccaga cggcgtgcgc gtcgccctgg agggcgagga
    18181 acgccccgag acccgggacg tgctcgtgga ggaagacgac gggcgcccgc tgcagggcct
    18241 ttctctccag cgccgcgagg gccggctcgt cctccgggcc ggcctcgcgg acctcgatca
    18301 ctcgagccct ccgtcgcggg ggacgaggga gacgacggcg ggtgtcttgt ggctccccgg
    18361 ctcgcggccg agggccggga cgagctcgac ctcgatcgtg atccccggaa ggaggggacg
    18421 gaggaagtcc gcggcctcgg cgcgaagggc ctcgcgcctc gcctcgccgg cagacggctc
    18481 gggaacgacg aggagccgca cccgcgtggc cgtctcgtgc ctcacctgga acgcggcgac
    18541 ctcttcgagg cgctcgaggg ccgacgtcag cgcgaactga tggacgaccg ggcctccggg
    18601 gacccggatc gcctcggcgg ctcgcccctc gagtcgcgcg aggagcgggt aggccgcgag
    18661 cgggcaggtg cagcgctcct ccgcccacgc gccgaggtcg gcgaggccgg cgtagcggat
    18721 cacgggcgtc acgcggttcc gcaggtcggt gacgacgatc ctcccggtga cgccgggcgc
    18781 cgccggccgg tcctcgtcgt cgaggacctc gacgaccgtc tggtactcga gcgtgtggag
    18841 ccccttcccc tcgtggcact gcaccgcgat cccgccgacc tccgtcgcgc agtaggcctg
    18901 cgcgaggccg gcgcgcggaa aggtgcggcg gatcgacgcg acgacccggg ggttcaggct
    18961 ctccccggag aagacgaact gctcgggctg ccacggggcg acgccgcgcc gccgggcctc
    19021 ggtggcgatc gcgccgacga tccccgggta gctgaggacg tactgcggcc gtctccggtt
    19081 cagctcctcg atcaggacct cgaccggcga gagggcgtcg aggaagacct cctccgcggc
    19141 cgggaactcc cggagcgcct gcccctcgat cgcctgcggc gagcggacca tgacgtggag
    19201 gccgcgctcc aggcgcagcc cgagatgcat ctccatcgag gggcgggaga agagggcgat
    19261 cgtgaagagc accttctcgc ggtcgagcgc cacgcgcacc ggcgtccccg tcgagccggt
    19321 ggtccggatc tcgcgggccc gccgaagcgc cgcggggtcg gcgcagaggc cgtcgggcca
    19381 cgccgaccga agctgctcct tcgacacgac cggaaggaga gacgggtcgc ggagcgccga
    19441 aaggtcgtcc tcgcggagag aggcggcgct ccagagctcc cgatagaacg gcgagcggcg
    19501 gaaggcgtgg aggcagacgg cacggagcgc cgccgcgcgg agctccttca cctccggcgc
    19561 cgtcgcgcgg agccgtcggc ggatcccggc gaggtcccgc ttcacgcggg cgaccttcgc
    19621 cagcatgccg aggccgggga ggtcgtagag ccggtccatc tttcgtcctc ggagcccctc
    19681 aggccgcggg ccgcgcggcg cagcgctcga gctcgcggcc gacgcggttc aggaagtagg
    19741 cgaggcggac gaaggcgttc gtcaggtcga agaggagcgc ccgttccctc tcgtcgagcg
    19801 acgccgagcc ggcgaagacg cgctcgcgga gctgctccat cgccgcgccg cggtctccgg
    19861 tcagcgcgac gagccggcgg acgtcgtcct cctcgcgcga ggcgagcgcg tcgacgccgg
    19921 cgaggaggag gaactccgcc gactgcgcga ccggatcgaa gaggcgggga aggcgccggg
    19981 ccgccgggtc gccgaggacg agcaggagcc cgccgagcgt ctcgtcgagc gagacgagcg
    20041 ctccgacgat ctccccgacg gcgtcgagac ggctccggag ggcctccggg aggcggccgg
    20101 ccgagaggcg ggccagctcg cgccgggcgc cggacgcgcg gacctcgaac gccgcgtcga
    20161 acggagtcgc cgctccctcg gggagcgatc cgccctcccc cgcgacgcgc gcgagccggg
    20221 gagggaggag gcgggccagc tcggcgagct cccgcagcag gcgctcgagg ccgaggtgcg
    20281 tctcctccac gaggccgtcc tcgatccgga tgatgcggtc cgccacgtcc aggatccggt
    20341 cgtcgtgcgt gacgaggagg atcgtgcagc cggtctggcg cgcccgccgc tggaggaggt
    20401 cgacgacctc ccggcccgtc ttcccgtcca gcgcggcggt cggctcgtcg gcgaggatga
    20461 tccggggctt gcgagccagg gcgcgggcga tcgcgacgcg ctgcttctgc cccccggaga
    20521 gctggcgcgg gagcttcccg gcgtgggctt cgaggccgac gtcggcgagc gcctcgagcg
    20581 cccgctcgcg cgtcgtcgcc gccgtctcgt cgcccccgac gaacgccatc tggacgttct
    20641 ggcgggcggt gagggagtcg atcaggttgt gggcctggaa gacgaacccg atccgcttgc
    20701 ggacccgcgc gagctccgcg gcgccgagcc tcccgagctc gacgccctcg acgcggacgg
    20761 tcccctcctg gaccgaccgg agcgccccga cgagcgtcag gaacgtcgtc ttgccggagc
    20821 ccgagggccc ggtgaggagg acgatctcgc cggcgtggaa ctccgtgctg acgccgtgaa
    20881 ggacctcccg gcgcagggcg ccctcgccga aggagtgccg aaggtcgcgg acggagatga
    20941 cggcccccac gcctcctcct cagaagacgt ccgccgggtc ggcgttgcgg agccgtcgga
    21001 gcgcgaggac gccggcgagc gcgcacatga ggaacgcgag gacgaggacg agggagacgt
    21061 ttcccgccgt caggtaggcg ggcatcacgg ccttcgagcg cgccaccgcg gagaggagcg
    21121 cggtggcgag ggtgcccggc acgaagccgc acgccatcag gatcacggcc tgctggagga
    21181 cgagggagag gaagaagcgg tcgcccgcgc cgatcgcctt gagggtggcg tactcgtgga
    21241 tgtggtcgct cacgtccgtg tagaggacct ggtagacgat cacggccccc acggtcatgg
    21301 ccacgagcat cgtcccgagg aagacgaagg cgagcgggga gcgctcgttc cagtacctct
    21361 tctcctccgc gacgaaggcc tcgtgggtga agaccgtgac gtcccgcggg agggcggcgc
    21421 gcagccgctc cgccgcgagg gcggcgtcga cgccgggccg gagccgcacc aggccgacgt
    21481 tgatcatgtt gcgagggtgg gccgggacga gccggaagta ggcctcctct cccgcaagga
    21541 cgtgcccgcc ggaggagatc gtgccgccca tcgagaagag cccccggatc acgactcgcc
    21601 ggtcgccgat ctccgtctcg accgggcccc gctcggcgag gagccgcgcg atcggcccgt
    21661 actctttgag ccccctgcgg tcgaagagga cgtcctccgt ggtcgagagg acgccgaggc
    21721 tcctttccac ctccggaagg gcgaaggggt tccgcgacgg gaggatcccg aagacgcaga
    21781 tccgcatgtt ctgacgcgtc accgggttcc gcatcgtcgc gctgccgacc cagagggggg
    21841 cgacggactc gaccccctcg accgcgagcg cctgctccag ccgccggcgc gtgaagaggc
    21901 cgttggagta gaggttcagg tagtcgcggc tcgtcaggac gaggtcgccg cggagcgcac
    21961 ggtgcgggta gacgaccttc tcgaagatcg cgacgtagat ccccatctgg aagagcatga
    22021 gcgtcacggc gaacgtgacg ccggcgaccg cggcgaggaa gcgcttccgc tcggcggcga
    22081 gctgccgcca cgcgagcggg acgccgagcg ggaagagacg gtcccgcgcc gccgcgagga
    22141 gcccgctcat ggcccgatcg tcacgtcgac ctgcgcgttg ctcagcgagg cgagcgtccg
    22201 cccctcgtcg aggcggacca ggaccttcac gacgcgccgg tcggcccagg agttcgggtc
    22261 ggcctccctc agggcgttgc cgcccacctg gccgacgacc tcggtcacgc ggcccgagag
    22321 ctcgccggga acgccgtcgc cgcgaacgcg caccggcgag ccgacccgga cgcgcgccac
    22381 gtccgtcacg tagacctcgg cctcgacgcg catcgcgtcg gtgtcgccca ggtcgaggac
    22441 gccccgcgtc cccacggtct cgccgggcca cgcggcgacg tcgaggacca caccgtctat
    22501 cggcgcccgg aggacgcaga ggtcggcctc ggcgcgggcg cgctcgaggg cggccttcgc
    22561 cgaggcgagg cggcgctcgg cgacggcgat ctccgtcggt cgggaggtcc tgagggcgac
    22621 gagccgccgg gacgcctcct cctccgtcgt ctcggcgcgg cggaggtcca gctccgccgc
    22681 ctcccggtcg ctctcgggga ggagcccttc ggcccggagg cgctccgcgc gcgccctgtc
    22741 ggccttcgcc ttctcgacct ccctccgcgc gcgctcgagg ccggcctcga gcgcggcgat
    22801 cgcggagagc ttctcctcgc cgcgcgcctg cgcgaggagc acctcggcca cccgcacctc
    22861 ggcctcggcc tggcgtgcgg cggcgtcggc cgcgtcgaag ctccgaaaga cgacgagcgc
    22921 ctggcccttc cggacccggt cgttccggcg gacgagcagc tccttgacga ccgactgcgg
    22981 cggcgcggcg agcgccagca ccttctcgcc ggggacgacc cgtccgaggc agtggaccgc
    23041 ccgggccggg gcgtccgcgg gggcggcttc ggccccctcc cgggcgcggc ctttcccgca
    23101 ggccgcggcg aggacgacga ggaggccggc gacggcggcc ttcgcggcga cgcaccggca
    23161 gcgcgcggcg cctcgggaga tgcggtcccg cccgctggtc gactcgcgac tgacgttcac
    23221 ggcatccccc gcatggagtg gatttcgccc gaggagcaga ggtgggtccc gaggaaacgg
    23281 ccggccgccc tgggagtcgg attgtccgcc ggctctcgga gggcggcaag cgtgccgatc
    23341 gacggcccga ccggtctctc cgggggcgtc tggtaaggca tcggtccggg gccctgcggc
    23401 gtccgggctc gcgccgccgt gcggtccctt acgggggtgg gccccgtcgc gacgggtcgc
    23461 tgatgggggg cgggcgcctg atgcgggagg tccagcaggt cgtggcgctc ccgaaaccgt
    23521 cgccgcgtcc gcattcagcc cgcgccccct ccgcctcgta tccgagcttc ggggcgagcc
    23581 agcgctcgac ctcggcgagc ggcatcccct tccgccgcgc gtactcggcg acctggtcct
    23641 tgccgaggcg gccgacggcg aaggagcggg cctcggggtg cgcgaggtcg gggccgcgga
    23701 cggaggccgg cggaagcctc gcgaggctct ccgtgagcgt gatgccggcg cgggacggcg
    23761 cgtcgaggag ggagaagagg gttcgcttct ccgtgtggtc ggggcaggcg gggtaaacgg
    23821 gagcgggacg gatgccgcgg taacgctcgc ggaccaggtc gtcgtgcgag agaggctcgg
    23881 cgcctatgtc ggcgaagggc tcgcgcgccc tctcgccgac gacggcgtcg tcggtcaccg
    23941 accgaagacc ggtccttgac ggcacttcga gcgggcaata cgcttctccg aactgcctca
    24001 aggagcggcc tgacgttggt cgagatcctc ggtgtccggt tcgacgagct gaccgaggcg
    24061 caaaccgtcc gacgaatcct cgacgcgatc gacgagagac gcggcggcgt cgtcgtcacc
    24121 ccgaacctcg accatctgcg tcgctgcagc atcgacgcga cctaccgaac gctcgtcgcg
    24181 aaggccgacc tcgtcgtcgc ggacggcatg ccgatcgtgt gggcgagccg cgtcgcgggg
    24241 cagcctcttc ccgaacgcgt gaccggcgcc gacctcgtcg cgagcctttc cgccggagcg
    24301 gcggaacgcg ggcgctccgt gttcctcctc ggcggcgacc cggggacggc cgagggcgct
    24361 gcccgcgtcc tgagcgaggg gactccgcgc ctgaaggtcg cggggacctg gttccctccc
    24421 cggggcttcg aacgcgaccc ggtggagtcg cgacggctcg aggcggcggt gtccgccgcg
    24481 gcaccggacg tggtgttcgt cggcctcggc tcgccgaagc aggagctgct gatcgaccgg
    24541 atccggtccc tgctcccggc ggcctggtgg ctcggcgtgg gcatctcgtt cagcctcctc
    24601 accggagagg agaagcgggc cccggcctgg atgcggcgtt cgggcttcga atggctgcac
    24661 cggctcgcct gtgagcctcg gcggctgtat cgccgatacc tggtcgatgg ccttccgttc
    24721 gcgcttcgcc tggccgcctg gagcgtgagg cgccggttcc ggcggcgagg cccggcgcac
    24781 gacgggaccg ccgcgccggg cggcggccgg gacggcccgg tgaatcgctg cgtcgagggc
    24841 tcgcgcccgg cgtgacgcgc gccgaggtcc ctccgtggtg cctcgcggga atggcgctcg
    24901 tgccgtacgc gacggtcccg ctctcgctcg aggccgggct gcggacgcag gccgggctgc
    24961 tcgtcatcgc tctcgtcgtc gtggcctgga gcggtcggca gtctcttcgg gatgcgttcg
    25021 ggcgcctgcc gccggccgtg cgcgtcgggc tctgcctcta cgccggcgcc gccctgtggg
    25081 gcgccgtcgt cggagctttc gccgcccacc cgtcgcgatc ggtcgtcagc cagctggtct
    25141 ccctgtggct cctgcccgcg gcggcgctcg cgttcggcaa gcacccggac ctcgacgggg
    25201 aggcgctcgt gcgggccctg gggtgggccg ccacggcggc cgccgccttc cacgcggtcg
    25261 ggctcgccgt gccccggctc gccggggacc tctcgggaca cttcggcttc gagctgcgca
    25321 acagcatcgg gatcgtcggg ctggcccccc tgctctggct cgtcggtctg gcgctggtct
    25381 gtgcccgcac ccagccggcc tcggcgctcg ttccggtcgt cgcggcgggg gtcctctgct
    25441 tctgtgccca gtcgcgtggc ggttggctga tctcggccgc aggatcgctc ggcattctcc
    25501 tgctcgtgca gcgccgttcg atcctcgtcc gtcttctcgt cggcgcgctc gccgtcgcgc
    25561 cgctcgtcgc gacggccgcc gcgtgggcat ccgcaagggc cgagcccgtg ccggtcgtcg
    25621 acgggcgcgc gcccacccgg ctcgagtcgc cggacgctgc cggagccttc agcgaggcgt
    25681 tcggggaggg ggccatcgag ggagccgctc tggaggtctc ggcggtcctg cgcggcgttc
    25741 gtggccggcg actgtgggtc tgggccgact tttccgacga agagggacgg gcgctcggac
    25801 gccagtcggc cgaggtgatc ggcacgggag actggcgggg ctggagcgag ctgctcgcgg
    25861 cgccgcccgg ggcacgttcg ttccgggccg gccttcgact ggatccgggg cagggagcgt
    25921 gggaagccga aggcgtgagc gccacggtcg tgaagtcccg gctcgtgggc ctcggccggc
    25981 agctccggcg ccgcgcctcg agcctcggcc gggtgctcgc ggatccgggc gccgacagcg
    26041 cgatcgccta ccgcctcgcc gaggcccggc tcgtcctcga cgggtggcgg cgggccggaa
    26101 cggctcgccg gatcgccggg ttcggcctcg gggcgaccat cgagctgccc cacgccacgt
    26161 tcgacgacga gggccggcgt gcggtcgcgc cgaggagcag ctacctccac aacttctacc
    26221 tcttcctcct ttacaagctg ggcctgggcg gcgccgccgc cctcgccggt ctcgcgctga
    26281 tcacggggtg gaccgtacgc tcggcgctct ccctgcgagc ccggccggag agctcgtggc
    26341 tgctcgcggg cgtcggagtc gccctgctcg cctatctcgt ctggagcctg acgtctcccg
    26401 agatctacga ctttcgcatc gccccgctct ggggggcgat gctcgcggcg tgcggccgcg
    26461 cagcggagcg agggccgtcg gtcgggcccg caggccccgg ggacgggcgc ccggcgtgag
    26521 cgcgcgagcg atccggagcc ttctccgatc ggcgcagcgg gcgcccgtcg tcgacgcgct
    26581 gtcggcgacc ggcttcaccc tcgtcggcaa gtcggtgggc ttcctcgttc cgctcttcgt
    26641 cgcggcgcag ttcggcgtca ccgccgcgac cgacgcgttc ttcttcgttc tcggcaccgt
    26701 cctcgcggtg accggcgtgc tggccgccct gctcgagctc gtcgttccct ccgtcgcgga
    26761 gctgcgcggc cgggacgagg agctcgccgc gttcatgaac gggcttctgg gcacggccac
    26821 gctcgctgtc gcgctcggtt tcgccgcttt cctggccgtc ctcggccctc tcctgcccgt
    26881 cgtcacgagg ttccccgccg gcgacctcgc gctcgtggcc cgcctcctgc tcgagacctc
    26941 gccgctcgtc ctcctggtcc tctggggcag cgtcctctcg gggtacctca acgcgcacag
    27001 ggtcttcggc gtcccggcgt tgacgccggc cgttcgtggt gcggtcgcca tcgcgtgcgt
    27061 cctcctcttc ggcgacacgc tcggggtcca cgcgctgccg ctgggctacg tcgcgggaga
    27121 gacggcgcgc ctcgtcgtcc tcggcgtcgc ggtgcggcgc cgagcaccct tcccgctgcg
    27181 cccggagctt cgccgggact ctcggctcgc cgggttctac cgggcgctcg ggtacccagc
    27241 cgtcgcgacg gtgctgaccc tggtgaaccc gttcgtcgac agcatcgcgg cctcgtggct
    27301 ggctcccggg agcgtgacgg tcctgcgcta cgcggagagc ctctacatga tcccgttcag
    27361 cctcttcacc gggggcctgg tccccgtctt cctggcgcat tggagcgaca gggcccgtga
    27421 cggggagctg gccgcgctcc gccgcgacgt cacgcggagc gcgcggctcg tcctctggct
    27481 caccgtcgcc gtggcgacgg tcctcgccct gctgagccgt cccatcgcgt cgctccttct
    27541 cgcacacggc tcgttcgacg ccgccaaggt gccccaggtc gcgctcgtct gggcctgctg
    27601 tctggccgcg ttcccggcgc aggccgtcgc ccagctccac gtcaaggcgc tcttcgtcct
    27661 ccggcggacg aggatcgtga tggtctgcgc gctgtgcgtc gctccgctca acctcgtcct
    27721 cgacctcctg ctgatgcggg tgctcggcgt cgccgggatc gcgctgtcga ccgccggcac
    27781 cgcgctcgtc acgctggcga tcgtgtcgag cggccttcgg acggcgttcg tcgagcgggc
    27841 ccgcggcgca ggaggtggcg atgtcccgcc ctccccctga cgccgagcag acgccggccg
    27901 tcgtccacct cctcaacggc gacctcttcg gcggcgtgga gcgcgtcgtc gcgacgctct
    27961 gccggaaccg ccgccgcgtc cggtccgagg ccgtctgcct gctggacggg gagatgcggc
    28021 gcgtgtccgg ggtcgcctgc gagctcgtcc cgatggccgg gcgcctcgac gtgggcgtcg
    28081 cggtccgcgt cgcccgcctc atccgccggc gtcgtggccg cctcctcgtc acccacactc
    28141 ttcggtccag cctcgtcgga gcgctcgcag cgcgcttcgc gaacgtgcct cacgtggtga
    28201 cggtccacag tccggctgcg cgcgagaccg gcgcgcgcct tcgcgatgcc cggaacgggg
    28261 ccctccagcg tctcctcctc ccgtgggccg acgccatcgt ggcggtgagc gcggcgttgc
    28321 gcgacgagct cgtgtcggcc ggcattccgg catcgcggat cgaggtggtc ctcaacgggg
    28381 tcgagacggg cctctgcgat ggcggcgatg cctccgcgtt ccggacctgc ctcccgggcg
    28441 gacggcgggc ggcgccgctc gtgggaacgc acgccctcct gcggccacgc aagggcctgg
    28501 aggacctgct ccgcgccgcg ccggcgatcc tccgggcgca cccctcgtgc accttcgtcg
    28561 tcgtcggagg gccggagcgg ccggactatc cgtcggccct gcggcagctg tgcctcgacc
    28621 tcgggatcgc cgactcggtc gtgttcacag gacaccggga cgacatcgcc gacgtgctcg
    28681 cggccctcga cgtcttcgtc ctaccgtccc tcttcggcga ggggcttccg ctcgcgctcc
    28741 tcgaggcgat ggccgcgggc cgtgcggccg tcgccacgga caccgaaggc atacgggagg
    28801 tcgtcgagag cggccgcacg ggtctcctcg ttccgccccg ggaccccgcg agcctcgcgg
    28861 gcgccgtgaa cgccctcctg agcgaccggt cgcgccggag cgagctgggt tccgccgcaa
    28921 gggaggccgc tcgacgccga ttcggcgtcg ggcgaatgac ggctgaggcc gaggcggcct
    28981 atcttcgcgt cctggccgcg aggacgtcgc gatggagctc ggcgtagagc ccggcctggc
    29041 gccaggggtc gtgccgcgag aggacgcggg cccgggcggc gcggccgagc gcgtccgccc
    29101 gcacgggatc cgagaggagg tcggccacgc agcgggcgag cgcgcctcgc tccagcggtg
    29161 agaccaggac gcccgtgacg ccgtcctcga cgaggctgcg gttcgcccgg atgtcgagcg
    29221 cgacgacggc ccgccctgcg gccatcgcct cgaggatcga cagcgacatc ccctcgcgtt
    29281 ccgacggcag gcagacgacg tccgcaacgg cgaggagccc ggccacgtcg tcgcggtcgc
    29341 cgaggaaccg gacccggtcg gcgacgccgg tgtgacgcgc cagagcctcg aggtcccgcc
    29401 gctcgggccc gtcgccggcg agcatcgcca cggcggccgg gaccctcgcg gccaccgccg
    29461 gcatcgcttc gaccaggcgt cggcacccct tctcggcgga gaggcgtccg acggacagga
    29521 cgacccgggc gtcggcgtcc agcccgagag cctgacgcag gaaggccgtc ggcccggcgg
    29581 gctcgtgctt ccgcgggctc ggcgcgttct cgatcacccg cgtcctgccg ccggccacgg
    29641 cgccgagctg gcgggccacc gactcccgga cggcctccga gaccgcgacg atcgcgtcga
    29701 cgcggcgcgc cgcgtgccgg tagcgtgccc ggcacgcgag ccggcgcagg ggcaacgatc
    29761 gacccggccg cagcggaagg acgtcggtcc cgtggaacgt ccagatcacc cccggtgcgc
    29821 ggggcgtccg gtgcaggctc aggatcgccg ggtcgaggac gggcagcagg tgcgtctgca
    29881 cgatgtcggc gccccactcc tcgacgcatg cggcgagcgc gcccaggacc ttccggagct
    29941 ccgagaccca gcggaccggc cgggcgaacg tgacgcgcgg accgggaacg accctgcaga
    30001 gggcgccgcc ggcctccagc ctggcgcgga gaggaccgtc ggcgagcgtg cagacgagga
    30061 tctccgcagg ggccggccgg cactcggcga gcgtgcagag gagctcctgc gcgcccccgc
    30121 gctcgaggtt cgcgatcacg tgcaggaccc tcggctcacg catcgggccg atcgtcctcc
    30181 acgcggacgg cgatcaaccc gaggacgagg cccgcgactc cgagcgcggt cgtcacgtag
    30241 cgcaggtcca cgagaacgct cacgaccgcc aggaacgccg cgaccgccca gagcctcgtc
    30301 agcgcgcgat cccggcggcg ccggcgcacg gtggcggtgg cgagagccgc cgccggagcg
    30361 acgaacagaa cgtacccgag caggcccatc tcggcgagga cggagacgtg cgtgcagtgc
    30421 gaggccgccc agcgccggtc ccattcgctc gcgacgaacg gcccgacgtc ggtcacgtgc
    30481 gcacgggcgt gccggtcgta cgtcccgaac ccccagccga gcagaggttt ctgcaggaac
    30541 atccgcagcc ccgccgatgc cagcacgatc cggtcggcga tcgttcccga catcttcaga
    30601 cgggtcaccg cgtagggctc gcgcgccggc gtcgcgtccc gcgaagcggg ggccgccgcg
    30661 acgagaggcg attcggtggc agggaccttc gcctcccgcg acggcacctg cgagaggccg
    30721 acgaccgcga gcgcgatcgc gagacacggg agcgcgacgg agcggccgca ggccgccacg
    30781 aggagcgcga gcgcgagcgc gccgcccagc cagctcgcac gggagaagct caggaagacg
    30841 ccgacgaacg cgagagcgaa gccggtggag gcggcccagc gcatcgcggg gctcgcggcc
    30901 gtccgtcccg tccgcgcgag cagtgtcgcg gcgaagacga ggacggccga gtacaggtcc
    30961 ggctgcgtca acgtgccgac gacccgcaac ccgccggtct cctcgacggc tcccggccag
    31021 aaggccggaa gcgacgcggg cgagaaccac gccaggagcc cgacggccag ctcgacggcg
    31081 cacagcgccg acagcggcgc cacccatccg tcgaagtcct cctcgcgcag gcgggccgcc
    31141 cgaacggccc agaacgccgc cgcgggaacc acgaacgccc gccacaggtc ggccgcgtcg
    31201 cgaggcgacg ccgcgccggc gacgctccgc agcgcgagag agatcgcggc gaaagcgagg
    31261 aagccggaca gggccgcgtc cgggagcgaa agccggaacg tcctctgcac gcgggaactc
    31321 gcggcctgcc ccacgaggac cgtcgcggtc agcacgggaa acagaccgta ggtgttgaag
    31381 aggtaggccg gccgaagagc ggaatcacga ggaatcaacg cggcggcgag cagccagatg
    31441 accgagacgc ccgcaaccag ccgtgcgagc ccgcgtcgca tcgcagaagt atagcggggc
    31501 tagactctcg ggccggccga ccgggaaggt ccgtgcggcc cggaggccgc cggaacgcgg
    31561 cggtgacgga tgagcggcga cacgagcagc gaggcgcagc ggcgggagca ctacgagatc
    31621 gagcgggagc tcgcggcgcg gcttcgggcc gcgccgcgcg aggagaggcg gcggctctac
    31681 gcggcgctct acgacgagct gtaccggcgc gttccccacc acccgcagag ggcggcgccc
    31741 tcctcggcgg cggagacggc gcgcgcggtc cggcgtgaga tcgcggtgct gcgccccttc
    31801 ctctcgccgg cgagcacgtt cctggagctc gggcccggcg cctgtgccct ttcgctcgcg
    31861 gtcagcccgc tcgtcgcccg cgtctgggcg gtggacgtca cggacgcgct cgtcccgccg
    31921 cggccccatc cgcccggctt cgctttcgtc cgctcggacg gctgcagcgt gccggtcccg
    31981 ccggggagcg tgaacgtcgc ctacagccac caggtcctcg agcacctgca cccggaggac
    32041 gctctcgcgc agctcgtgag cacccgcgac gcgctcgccg ccggcggcgt gtacgtctgc
    32101 gtcacgccga accgcctcag cggtccgcac gacgtctcac gcggcttcga cccggtcgcg
    32161 acgggcttcc acctgaagga gtacacggcc gccgagctgc ggacgctcct cctcgcggcc
    32221 gggttcgcac gtgtcgaggc gctcgtcggg gcgcgcggac ggttcgtgcg cctcccggcc
    32281 gccgcggtcg taccgtgtga acgggcgctc caggcgatcc cgacggcgtt tgcgcgccct
    32341 ctcgcccgca gcgcgccgtt ccgcgccctc ctcggcctgc gcctcgtcgc gttccggtga
    32401 gccggcgctt tctccgaacg atcgggcggg gaggtgatgc gggctccgac gcgtccggcg
    32461 atgggcacgg gtgcagcgga cgccccgacc ccgtctccgg acggcggcgg tcgccgatcg
    32521 ggacgtcgac gcgggtggcc gtcgaggaag gtagtcgaca cgggctcgga ctccggcgag
    32581 gacgtccacg ggtcaggcgg gagaggaacc gggccggagt cacaggatca ggccgtgccg
    32641 agaggtctcg agcacgtcga tgcggcgccc gcaggaggtc agaggtcgac gagaccggtc
    32701 gacgctgacg ggagcgacag gggtgctccg ttcaggcccc cgtcccctcc gcctcgtacc
    32761 cgaggttcgg ggcgagccag cgctcggcct cggcgagcgg catcccctta cggcgggcgt
    32821 agtcggcgac ctggtcgcgg ccgaggcggc cgacggcgaa gtagtgcgcc tcggggtgtg
    32881 cgaggtagag gccgctgacg gaggcggtcg ggagcatcgc gaggctttcc gtgagcgtga
    32941 tgccggtgcg cgcgggggcg tcgagaagag cgaagagtgt cctcttctcg gtgtggtcgg
    33001 ggcaggcggg gtagccgggg gcggggcgga tgccgcggta gcgttctcgg atcaggtcgt
    33061 cgtgcgagag gcgctcgccg aagccccact cctcgcgggc gcgctcgtgg agccgctccg
    33121 cgaaggcctc ggccaggcgg tcggcgagcg ccttcgcgag gatcgaacgg tagtcgtcgt
    33181 ggtctttctc gaagcgctcg cagagcgcgg ggaggccgtg gcccgcggtg acggcgaaga
    33241 ggccgacgtg gtcgcggcgg ccggaggtcc ggggcgccac gaagtcggcg agcgcctcgt
    33301 acggggcatc tccgctcttc tcctgctgct ggcggagggt gtggagcgtc gcgaggaggt
    33361 gctcgcccgc ggcgtcggcg tagagctcga cgtcgtcgcc gacggagttc gcggggaaga
    33421 agccccaggc gccacgggcc gagaggagct tcccgtcgac gatctcggcg aggagcgcct
    33481 gcgcgtcggc gaagagctcg cgggccttct cgccgatcgt cgcgtcgtcg aggatctgcg
    33541 ggtagcggcc gcgaagctcc caggcgtgga agaacggcga ccagtcgacg tatgggacga
    33601 gatcggtcag ggccggctcc tcgacgcgga cgccggtgaa cgaggggacg gggaggtcgg
    33661 ccgcagacca gtcgagggcg cggcgacgcg cgcgggccga ggagagcggg aggaggggtc
    33721 tcgtctgccg gcccgcgtgg tccctccgca gctcctcctg gagcgccgcg ttcttcgcga
    33781 ggaaggcggg gcggagctcg tcggacagga ggttccctgc gacgccgacg gcgcggctcg
    33841 cgtcggcgac gtggacgacg gcctgcgggg aagccggtgc gatccgcacg gcggtgtggg
    33901 cggggctcgt cgtggcgccg ccgatcagga tcgagaccgc gatcccctcg cgggagagct
    33961 cgcggacggt gtggaccatc tcgtcgaggg acggcgtgat gaggcccgag aggccgacga
    34021 ggttcgcccc cgtctcccgc gcggctttcg cgatccggtc ggcggcgacc atgacgccga
    34081 ggtcgatcac ctcgtagccg ttgcacgcga ggacgacgcc gacgatgttc ttgccgatgt
    34141 cgtggacgtc ccccttcacc gtggcgagga ggaccttccc ggcggccttc atcgttccgg
    34201 ccgccttccg cgcctcgagg aagggcgtca ggacggcgac cgacttcttc atgacgcggg
    34261 cgctcttgac gacctgcggg aggaacatct tccccgagcc gaagaggtcg ccgacgacgt
    34321 tcatcccggc catgagcggc ccctcgatga cggcgagcgg cgcgccgagc ttctcgagcg
    34381 cctcgagcgt gtcggcctcg acgtggtcgg cgttcccctt cacgagggcg tgggcgaggc
    34441 gctcctcgac ggacgccgat cgccacgcct ccgcgtgaac ggcggcgtgc gcggcgcccc
    34501 cctccttcgc cttcagcgtc tcggcgtacg cgacgagtcg ctccgtcgcg tcggggcgcc
    34561 ggttcaggag gacgtcctcc accatcccga ggagctcctt cgggacctcc tcgtagaccg
    34621 cgagctggcc ggcgttgacg atccccatgt ccatgccggc ggcgatggcg tggtagagga
    34681 aggccgcgtg catcgcctcg cgtaccgggt tgttgccgcg gaacgagaac gagacgttcg
    34741 agacgccgcc ggagaccttc gcgcggggaa gctcggcctt gatccgccgc gtcgcctcga
    34801 agtaggcgag ggcgtagtcg gcgtgctcct cgatcccggt gccgacggtc aggacgttcg
    34861 ggtcgaagat gacgtcctcg ggagggaagc cctcctcctc gacgaggatc cggtaggcgc
    34921 gccggcagac ctcgaccttc ctctcggccg tgtcggcctg gcccgcctcg tcgaacgcca
    34981 tgacgacggc cgcggcgccg tgccggcgca ccgtccgggc gcgctcccga aacgcagcct
    35041 cgccgtcctt caggctgatg gagttgacga tcgacttccc ctggagggac tggagccccg
    35101 cctcgatgac ctcccagcgg gagctgtcga tcatgacggg gagcttcgcg atgtcggggt
    35161 cggccgagag gaggttcagg aagcggcgca tggcggagac cgaatcgagc atcccctcgt
    35221 ccatgcagac gtcgaggacg ttcgcgcctc cctccacctg ctgccgcgcg acgccgagcg
    35281 ctccctcgta gtcgttcgcg gcgacgagct tcgcgaactt cggcgagccg gtgacgttcg
    35341 tccgctcgcc gacgacgatg aagttggaat cggggcggat cgtgagcggc tcgaggccgg
    35401 agagacgcgt aagccgctcg acggccggca ccgcgcgggg cggcagcccc ctcaccgcct
    35461 ccgcgatcgc gcggatgtgg ttcgggttcg tcccgcagca gccgccgacg aagttcaccc
    35521 agcccgcctc ggcgaacgcg cggaggtcct tcgccatcga ctcgggcgtc tcgtcgaagc
    35581 cgccgaaggc gttcgggagc cccgcgttcg ggtaggccga gaggaggacg ggcgcgatgc
    35641 gcgagagctc ctcgacgtag ggccgcattt ccttcgcgcc gagggcgcag ttgatgccga
    35701 ccgagaggag cggggcgtgc gagacggagt tccagaacgc ctcgacgagc tggccggaga
    35761 gggtccggcc gctccggtcg gtgatcgtca ccgagagcat gagcggcacg gcggtcccgc
    35821 gctcggcgaa gacctcctcg acggcgacga gggcggcctt gccgacgagc gtgtcgaaga
    35881 cggtctccac gaggaggagg tcgacgcccc cgtcgaggag gcctcggacc tggtcggcgt
    35941 aggccgtgac gaagtcggcg aagacgttcg tcctcttccc cgggtcgttc acgtcgacgg
    36001 cgagggagag ggtccgattc gtcggcccga tcgagcccgc gacccaggcg tgccggcccg
    36061 ggttcgcggc ctcgaaccca cggaccgcct cgcgcgcgac gcgcgcggcc gcggcgttca
    36121 gctcgcgggc gtgagcctcg aggccgtagt cggcgagcga gatcgcctgc gcgttgaacg
    36181 tgcaggtctc gatcacgtcc gcgccggcgt cgaggtaggc gcggtggatc tcggccacga
    36241 cgtgaggccg cgtgagcggg aggacgtcgg cgcatccctt cagctcgcgc gggtggtcgg
    36301 cgaagcgcgc gccgcgatac gccgcctcgt ccagccggta gggctggatc atcgtcccca
    36361 tcccgccgtc caggacgacg atccggtgcg cgagcgcctc ggcgagcgcg gggggcgcgg
    36421 gcgcggcggg cctcaattca cgcctccagg ggcgtccttc gcagcggcgt ccctcgcggc
    36481 ggcggaggga cgggcttccg cgtcgcgtcc cggcagcgac tcgaggacct tcctcacgac
    36541 ctcgtaccgg gccatcggca tcaggtggat cccgcgcgcc cgctccacga tcgccgcggc
    36601 gagctccacg ccgatcgcga gcccctcctc cgcctcgaac tcgggtccct tctcggccgc
    36661 cttccgcatc cggacgagga tcgcctcggg aacgaccatt ccggggacct cgttcgcgaa
    36721 gtagagcgcc tgcttcaggc tcttcggcgg gatgaggccg acgaggaccg ggatcgcccg
    36781 cgcctcgggg cgggcgaaga agcggtcgag cgccgcgacg tcgtagaccg gctgcgtctg
    36841 cgcgaacgtc gcccccgcct cgatcttcgc tctcagcttg gagatctcga cgtcgaggtc
    36901 gtgggccgcc gggttcgcag cgcaggcgat ggcgaacgcc gtcgaggttc cgatcggcgc
    36961 gcccgccagg tcgactccgc cgttcaggcc cttcacgatc cggaggaggc cgagcgtgtc
    37021 gacctcgccg accgcccgcc cctgcgggta gtcgccgatc ttcagcgggt ccccgccgac
    37081 gacgaggagc gccttgatgc cgaggcccgc ggcgccgagg aggtcggatt ggatgccgag
    37141 gacgttccgg tcgcgcggcg agaagtggaa gacggtgtcg acgcccgtct cgtgccggat
    37201 catgtgcgcg acggcgatcg aggacatccg gaggcgcgcc atcgggttgt cggtgacgtt
    37261 cacggcgtcg acgccgtagc tccggaggag ctgggcgccc tcgagggcga cggagcagtc
    37321 ggtccccttc ggcggctcga tctcgacggt gacgacgagg gccttcgggt cggtcagctt
    37381 ccgcttcagg accgaggtct cgagcgcctc gggctcgggg gcggcgggcg ccgcgacgaa
    37441 ggaccgggcg acccgctcca cggaggcgcg cctcttcccg gagacctcct tcgacatcgc
    37501 ggcgacgtgg gccggcgtcg tgccgcagca gccgccgacg atcgcggccc ccgccctcac
    37561 gaagtcgcgg gccacctccc ggaagtagtc gggggtggcg ggatagaccg tccgtcccga
    37621 gacgttccag gggtagccgg cgttcggcat cgccgagacc gggagagacg tctgcgagat
    37681 gacgcgctgg aagacctcga gcgtctcctg cgggccgatc gtgcagttca gccccaggat
    37741 gtcggccccc gcctcctcga gccgggcgaa tgctcccgcg acctcggtgc cgaaggtcgt
    37801 cttgccgtcc gagaggaacg tcatcgaggc gaggatcggg atctccttcg agaggtcgcg
    37861 cgcggcccgg atcgcctcga tcgcctcgag cgtcgaggag aacgtctcga ggagcaggag
    37921 gtcgaccttc tcctcgagga ggatccggat ctgctcggag aagagctcgc ggaccatcgc
    37981 cttcgtgagc gggccgtagg gcttcacgag ctggccgagc gggccgacgt cgccggccac
    38041 gaggacgtac tcgccggccg cggcctcgtg cgcgaggcgg acggcggtgc ggttgatctt
    38101 ctcgagggag tcggcccagt cgaacttcgc gagcttgacc cggttcgcgt cccaggtgtt
    38161 cgtcttcagg atctgggtcc ccgcctcgac gtagtcgtcg tagacctccc ggacgaggcc
    38221 ggtctgggcg atcgagaggg gggagcgcgg gctctcgggg gcggccccgc gcgagacgag
    38281 gagcgtcccc atcgccccgt cggcgaggag aacttcggtc ttcaggcgct cgcggatgtc
    38341 gtgcttcatg aaaaaacccc ttcgaggacg aaggggcgtt tcgctgggga tcgcttccgt
    38401 tcggcctcga tttcatctct ctccggccgt gccggagcgg gaattggcac ctgtcaccct
    38461 ccccctttcg ggggcgagga gccggttgcc gcggcatcgc agggcccgtc cctccgccgc
    38521 tcgtgatgaa acccggactc aaagagccgg acggagccta cgggaggcgg cttccggccg
    38581 tcaagaagac cgcgctcttc cggggggggg cggcggtcgc cggggtcccg gtgcgagttc
    38641 ggcctcgggc tcgacctcgg gctggtttag actcgcctcc cgtgagagtt ctcgtcgtcg
    38701 gctcgggggg tcgcgagcac gccctcgtct ggaagctcgc ccagtcgccg ctggtcacgg
    38761 agatcttctg cgccccgggg aatcccggaa tcgctcagat cgccacctgc cttccgatcg
    38821 gcgtcaccga catcgtcgag ctcgccgact tcgccgagaa gatgaaggtc gacctgacgg
    38881 tcgtcgggcc ggagctcccg ctcacgctcg gcctcgtcga cgagctgacg aaacggcggc
    38941 tcctcgcatt cggcccgaac aagcacgcgg ccgagctgga ggggtccaag gtcttctcca
    39001 aggccttcat gaggaagtac gagatcccga cggccgaggc ggaggtgtgc acgacgcgcg
    39061 ccgaggccga gaaggccgtg aaggcgttcg gtctgccgtg cgtcttcaag gccgacgggc
    39121 tcgccgccgg caagggggtc gtcatcgccc gggacgaggc cgagaaggag aaggcgctga
    39181 agctcttctt cggcgagcgg gtcttcggca gcgccggcga ccgggtcctc gtcgagcggt
    39241 tcgtcacggg cgacgaggtg agctgcatgg tcctctgcga cggcgagcgg gcgatcccgc
    39301 tcgcgaccgc gaaggactac aagcgggtct tcgacggcga ccgcgggccc aacacgggcg
    39361 gcatgggcgg gcacagcccc tccgtcttcg tcgatgcgac gaccgcgcgg gagatcctga
    39421 ccgacatcgt ccacccgacg ctccgcggca tggccgagga ggggcgtccg ttccgcggcg
    39481 tcctctacgt gggcctgatg ctcgagaacg ggatgaagcc gtacgtcctc gagtacaacg
    39541 tgaggttcgg cgaccccgag acgcagtgcg tgctggtccg cctcgaggac gacctcctcc
    39601 cgtacctcct cggctcggcg cgcgggaagt tcgaagagaa cgccaagccg gtctggaaga
    39661 aggaggctgg ggccgccgtc gtcctttctg ccgagggcta tccggggccg ttcgagcgcg
    39721 ggaagccgat cgaggggatc gaggacgcga cgtccgaaga aggggtcttc gtcttccacg
    39781 cg
//
LOCUS       DAOTEZ010000002        33768 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_855_length_33768_cov_6.332275, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000002 DAOTEZ010000000
VERSION     DAOTEZ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 33768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33768
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_855_length_33768_cov_6.332275"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="P5164_00145"
     CDS             complement(<1..1130)
                     /locus_tag="P5164_00145"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRY42325.1"
                     /translation="MTPAPPAARRRAIVVVGVVGALIALMSVADMFLPKAWDGVVPDT
                     YAESGLQVRAVVRGGPAEAAGIRPGDTILGIGRRMLNTPAEAASELGRQAAGETVPYL
                     IKRGEQVLEREVLLSPYRLGSASYVYYALLGGLFFGLGLFVYSRRAGDEAAEVFFLLC
                     VLFMLFFVCRLRPSSYYWIDWFVQVAGTLALFLLGAVFLHFFLLFPRRKVFRFAEARP
                     GEEPPAAPLVLLQRFLNGSPALFVILYTLPPAFYALQMAVLRRTGDRSALVYGAPRAN
                     WILLADYLVLGLLALAHSWWTAREKTTRRPILVLLLGTLGGIVPFVLFAVYFPSLFRD
                     ERYLAWGVVPMALVPLTFAYAIVRFRLFDVQVIVGKSIVYAIL"
     gene            1238..1873
                     /gene="tmk"
                     /locus_tag="P5164_00150"
     CDS             1238..1873
                     /gene="tmk"
                     /locus_tag="P5164_00150"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRY42326.1"
                     /translation="MTARSRRGLFVTFEGIEGSGKSTQLRILARRLRRLGVPLLVTRQ
                     PGGTPAGARLRRLLLHAGKLDPRAELLLMFADRRQHLTESIEPALSAGTLVLCDRYTD
                     ASRAYQGAGRDLGEELVDLLHARFCGLEPDRTYLFDCPPATGLARVAAARGGKDRIER
                     EALAFHRRVRAAYLARARREPDRFLVLDGTRPVEALAEQVAGDLEALRAAR"
     gene            1870..2829
                     /locus_tag="P5164_00155"
     CDS             1870..2829
                     /locus_tag="P5164_00155"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42327.1"
                     /translation="MTDVPVQLLTGRGSRRLEAEALDVAAALLCRSGDRTIECPDCRR
                     VARREHPDLLVAAPERARRANTPPFVESSESKETTIPTALVRAISADASRLPYEGTRR
                     VLVLLDVDRTESAAFSALLKVLEEPPSRARFVLTATRPRLLPPTILSRLVLRRLPAFS
                     REALVAALVAGGMPPEDASARAAFGPPGEEEARDLDLGAARETRDALLEAVSGLLLSR
                     STGWALVLAGRLAAEDAGATADRLSLLARLLRDAVAAGTDPAGRTVHHVERYRDLARL
                     SEAGVDVLLEAARRALREAGDLPDSRRNPRLAAEAFALSLRAL"
     gene            complement(2890..4257)
                     /locus_tag="P5164_00160"
     CDS             complement(2890..4257)
                     /locus_tag="P5164_00160"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HRY42328.1"
                     /translation="MTDPKLSRTSSRTATWSRPGLAGALVALPALLLAPTAFAQREPG
                     TASPSTGSAPAPSSSASAPSRSSPSPSYSAPAPSAPRSAPAPSAGSPSTPRRAAAPRE
                     TSAGAPARPGRGVVAAEPRDGRSGGSTSATAGGGGGRVPGVVAAHPRGPGSGSHRHPR
                     RRYYSPYTGWGGTSWWWGGPWDWWWWGSRFGPYHWGFGGVVIVEDYSPDPYRYARVDT
                     DVSPEAAEVYLDGTFIGSADDFDGYPDFLWLEAGKYTIEFRHPSYETLKHEVEVRPGQ
                     ALRFRDDLKLEPGKKRLEATEPPRRGTPHGRVFGKPEEPRRDRTGRFEARAEADRERD
                     REELDDLDELEELAEELKEADEDIAPAPKPRTDAKEGRSAREALPAERGRLRFEIQPA
                     DAAVYLDDEYIGTGKELAGLRRGVPADAGRHTVSVVRPGFVTKTIEVEAKPGAAIDVV
                     VELEK"
     gene            complement(4378..8451)
                     /locus_tag="P5164_00165"
     CDS             complement(4378..8451)
                     /locus_tag="P5164_00165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY42329.1"
                     /translation="MAPPSPDPFPERRLLPYETTRLEARRVLVLAPHADDETFGCGGT
                     LAALVDGGARVDVLVVTDGAGDEPDAEARRLVGARRLEETREALRRLGGGAARCAFLP
                     DRGLGRTGDPLAAAIESGLLAALPDLVFVPSPVEVHPDHRAVAEAFLSLFAGDAGRRL
                     VAALPEGARAAFFEVSQPFRPNVLVDVSMTIARKEAAVAAFVSQLDGHDYAAFARGLG
                     QYRRMSLPRSVASAEGFFLVALAELPAIDVHRLARAIGPTLSPGELFPATSPELPTVE
                     PAAGLPVGRGDGTPLVSVVLRTKDRPELLAEALASVAAETGVPLEAVVVNDGGADVSG
                     VLARFPGLRLRLVDLRPGRGRAAAANAGVEAATGRFVHFLDDDDLLLPGGLPALAAEA
                     REAEVVYGRVEAFRWSGTGEGRLRTPFRSFAEPFDDAALLLENFIPMSAPLLPREALL
                     AAGGLDAALERFEDWDLFLRLSQTHRFRLVDVPVSEYRVFPGHFVDDPDAATTQERDR
                     VAVLRKHAGRYGPEALSAILLHVKKDLLPREVRREVAAVETLLSRATEESVRERDRLE
                     AERADAERRAGRTLATRASVVVVNFDGRHHLEKCLPALEASDPKPGEVILVDNGSSDD
                     SVAWVRERHPAVKVLPMGANLGFGEANRRGALEARGDFLVLLNSDTVVEEGWLAGLLA
                     PLDAEPEIAATCSTLRLLATPELLNGLGGGMTRLAYAYDHGYRFPYAPWDPSGEEPRR
                     HDVLFPTAAAMAMRRHEFFGLGGFDPAFFMYHEDVDLGWRLWLLGRRVVVCRDSVVRH
                     AFLGTSKAVRGLDWRLKLGVRHAVRSILKYVEPVEAAKVLRWHAVLLARAGAWRLLAH
                     AVLWNLRHLPGTLRLRAETNARRKRSSEELRRAGLVLRALQPPPPPEAPRFREVRCDA
                     WIVSNVLLPGEPSGESRLGWGWYGPEHDGTASFRWTAGDARLGLRVAPGAEGRLKIVA
                     LGSPAAPAGPLRVTCNGRETTAELEPGAWRNLEVPVSAAADGFLDVVLRSPSGVPDDA
                     QGSWDFRRLGAGVRSVAFVPAAPEERHPLASVSVVIPTYERKAVLEETVRALASQTVR
                     PLEVIVVDDGSKDGTTELLHRLREELADRLDLVPLRQPNLKQGRARNLGVAHARGALV
                     LFLGDDTIPEPSCVAEHLAARASRPGACAVIGFTGWHRERMRVTPFLDFVNGHGPQFS
                     FDLLRDGQEVPFTTLYTSNVSIPRDLLGEEPFDHRFTSYGWEDCELGWRLSSAGLPIV
                     YRKAAATRHVHPQTMAQFLARQAHVGRAIDVLYEIHPELEGNRWLPPPRPRFRHRAFS
                     FAYRALGRVAARLDSAGVPLPLRVYHALVAWAFHSGR"
     gene            complement(8495..9556)
                     /locus_tag="P5164_00170"
     CDS             complement(8495..9556)
                     /locus_tag="P5164_00170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY42330.1"
                     /translation="MISAVFVSYRSAALAERAIATFRGEARRTGREAEVVVVVNSGDG
                     AERDALVPHADRVLLPPRNLGFAGGLNAGIAAARGGTLLLANPDLLFCEGSVAALVDA
                     AEAGGILAAGPALYADGARSVLLPPAEEGRPEELARRTLAADPARAARVFRRDARRAA
                     VQAERAAAGESAAVRGLSGALVAVTRAALEAVGPFDEAYPLYYEENDWQRRLLACGGG
                     LVYVGGAHVVHLFAQSTKTEPRVAAWFAESEQRYYAKHFGETGRRGLARLASCAPFEA
                     PPLPVRDGLSWDDAALAAVAISPFRHFRPFALALVPPGERRWTPPADLVAAHAGEALF
                     VRAFARTTGSTLAEARLAG"
     gene            9580..9735
                     /locus_tag="P5164_00175"
     CDS             9580..9735
                     /locus_tag="P5164_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42331.1"
                     /translation="MAIEFRPAGKSDRAAIMELFEVAFKAPASAGLLDRRAVFTSDAA
                     RVATRGS"
     gene            9735..11114
                     /locus_tag="P5164_00180"
     CDS             9735..11114
                     /locus_tag="P5164_00180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY42332.1"
                     /translation="MAFPDHGRPAPAPASGFALLALISTAGLALRLGFVHAIGFQPGF
                     FDVDGYHAMALAAANGASVPTSHHPPGYAWFLWLVYEALGVRPRIVYLLQAVLGAGAA
                     LLAGDSIRRVAGTSAGALAAALLAFNAYAVLATSTLASENLALPGVALVLWLSLDGSR
                     RPAAWRLLSAALVVSGLALVRTGLVLLAVPLAVLVLLPRPARGGSLSRRLAASLGVVL
                     VAFSGLFLHAVSRWRTTGVFRIGCPNDVYNLWVGNNPDATGRIDPMPVVPGVDLDKEA
                     QARLLAPRVRAFLRDHPLQQVRLFLARASYNFAPPKRDLIYVYGHGWAGERPRSVVLA
                     TYATVALSLPILLAVALLAFARSGHLPAFRLAAAMTAAVLLPYQVSIGDPRFLLPAYP
                     ALCAAAGLLGASRVPAAWSRGRRIVAALAMALFCANAARDLSLTDPALRAVSGPGGSR
                     LRPPYHFAR"
     gene            complement(11140..13071)
                     /locus_tag="P5164_00185"
     CDS             complement(11140..13071)
                     /locus_tag="P5164_00185"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HRY42333.1"
                     /translation="MISTAALSGSRTSTPPQPPESLRSGEPRRDAGPPADEAGGPEAR
                     DGCAPAPAGGRGRRPRRRAAGLIAVALVAIAGWVAGEHSRRSTWWTIDEPAHVKASRE
                     LREGRGVVSNFEHPVLMKVLGAAGLGAAPAGTAIETVRRARRLFPVVFGALLLVTGLW
                     VRARAGAACGAFAAGILLIEPSFRSSSGIVHTDVLLTFFLVSAAAALDRSTRGRRVSV
                     VGLVLSGLLYGGALVSKYSALPFLAAFAGVAWLRVAAAGRSPVSRALRAGPLARAAAL
                     TAVTVVTPALATLVAVQQAVTGPTPPEALRAAIRRSIADRGFHPRTSATELLSTAAER
                     LPRGLAAYAAGLEWVEKGSRPGGRLNYLFGEVSGDGFLLYFPVALALKMTTATVAFFG
                     LALLAAPGLVVRALRRGRSRLVGVACARTFVPLTLGLAYFVLAMAARLNIGVRHVMPA
                     TALLVVGVLIGLRAVSPFRPRARALLLAATALLAAVEAGPAWGRETSFGNVIAGGARG
                     LRGKLSDSNVDWGAGQGRMFERVRRGDLGRVAALTFPLWLDPDQRAQLAIDALEAPVT
                     AERICSYDSVFVSVYVWDSLEALMRNRETHATFESLRRSYGPALAVRGCAAAVEEVGP
                     EYLLFRVGRPAARGPSTTR"
     gene            13095..14060
                     /locus_tag="P5164_00190"
     CDS             13095..14060
                     /locus_tag="P5164_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42334.1"
                     /translation="MAIEFRPAGKSDRAAILELFEVAFKAPANAADLAWKYDVNPHPA
                     LSALAIEDGRALGFIGALGTRYRGEGMDQQGNTVVDVMTRPDSRALGRAGMFKRLGEL
                     FRRINAEAGTLFDFGFPNERARKIEERLFGCVLAEPCAERGRRLDAPPLLGRLRRRLL
                     RVSEGEPFGRAHEPLAELLHARPGWRTDRSVRTLAWRFSRPGFAYRTFQLLDWRGTSR
                     GYAVAAIREGRVALVDLQVASESDGTLVDLLAAIQEGLGAVAADRMVLRSPQRGLLAS
                     RLEGELAFVPELSDTVLLMHPIADGFDFLAPGSSFDYRFADHDVF"
     gene            complement(14080..15066)
                     /locus_tag="P5164_00195"
     CDS             complement(14080..15066)
                     /locus_tag="P5164_00195"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY42335.1"
                     /translation="MSDAVPQGAELEVRALFRDEAAETHAERRARLASDDPDLWEGIA
                     FPVEFALELAAVCNLACVMCPVPTTSRKRRLMDEALFRKAVDEVASETGFVLFPQGFG
                     EPMLHPKWAELLAHAKAKRIGPIVVLTNGTLLDERNVARLLDLDLDALVVSIDGVEPE
                     TYAAVRVGGDLGVVEANVRRFLAARGPAPRPRLVLRIIRMKETAAQISAFFERWRPLL
                     GPADEIAINEFNDWAGKVEDRSDAPRPQPAAAAAGRPPCRMLWMNLSVHADGKVSACC
                     HDSEDELIVGDLSKGDTVRGIWEGPALARLRQVHREGRFEELPICHACRNQA"
     gene            complement(15063..15983)
                     /locus_tag="P5164_00200"
     CDS             complement(15063..15983)
                     /locus_tag="P5164_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42336.1"
                     /translation="MADLKTTCETVVFCSSAGHCQLDCDYCIIHPVVKREPSLVYEDV
                     RFFLDEVGTRTFFIFSGKGDFFAGYPKRERFLDRLLDHDVEVALDVNGVLLNEYPELA
                     EEKLAKVRAVNLTLHYKQVRDKRVEKAWETNARTILEKHRGELILGTIFSPLLSDLWE
                     EGLAYYRDTVFAATGRKVWAIRDCDRPFDAAQEELFESLLERYAAVVEKEHKDDFSAP
                     FAGREAVLCPAGRDYFRIWNDGRVQGCPWIPGLADLGNLKERRLERRNAPFRCTTPRF
                     CDCYDIHQLGKMGYEDATPALPVLSSGAAR"
     gene            complement(16122..17195)
                     /locus_tag="P5164_00205"
     CDS             complement(16122..17195)
                     /locus_tag="P5164_00205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY42337.1"
                     /translation="MKVVYLLNGTALCGGVKVVYQHVAILRRLGVDAEVVSTTPPSDW
                     APESWSFYRQVPDLTPEAIGPADVAVGTMYHTVPQAMAVPGARAFHFCQAYEAIYEPA
                     RPLWPEIEEVYRLPATKLVVSPHLAELVKELYGQEAVHIPQPFDADAFSPRSGARPQD
                     GTFRVLLTGQWSVPFKGIPWATEALRALKGEIPRLRLVRMSQDAPPEEVAAWPEAERH
                     IWVPPAFVPDVYRNVDLFVGPSTEVEGFGLPTIEAMSCGLPTVLTDIGAFRGIDPDAR
                     ASIRIPVFDSGALQAAVRRLADDPGLRARLGTEGRRIGRTYHAGRTGEALLAAFGARP
                     AGAGSGLRRGARALASFVRGGAG"
     gene            complement(17192..18373)
                     /locus_tag="P5164_00210"
     CDS             complement(17192..18373)
                     /locus_tag="P5164_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY42338.1"
                     /translation="MSDAAFAFEGLSKVYRLYASPRDRLLEALTGRPRHTPVHALRGV
                     SATVPRGSVLGVIGENGSGKSTLLKILAGTTEPTSGTIHLPGRVAAILELGSSFHPEV
                     TGRRNAVLQAALAGLTKAEIAAALPEIEAFAELGDFFDRPVRTYSSGMTMRLAFAVAT
                     AVLPDVVILDEALAVGDGRFQKKCVDRLHDLRSRGRTIFLCSHALYYVSTLCETAIWL
                     RDGEVAALGEAQKVVLQYEEYLARKEQERTAAAPAALQDSLGRFTSIRLLDGAGRESA
                     VFAPGDPWVLELAFEGDTPERPLHVHVAIVTPDQVTCFVADSKVSGTGPFVGKARHEI
                     RLTVESLPLAKGEFILVIFLGDEKGLALYDARSDVRFRVESDRWTSGFMTVPVGWETL
                     G"
     gene            complement(18366..19484)
                     /locus_tag="P5164_00215"
     CDS             complement(18366..19484)
                     /locus_tag="P5164_00215"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY42339.1"
                     /translation="MNASPDDVSRSAAPLSGAARLVASPYLHFDGWQVVNPLTNVTLT
                     PADRLFAPLHDLRASRPVDGAIGRELQDGLWAIPDGTDLSTRFRLKVVSLEAHSVCNQ
                     ACYFCPVSVAPREKSFMPTELYERIVAELSAFRDTIEGVCMNNYNEPTVDRRFVQQVA
                     TIKAAGLPPAVLSNGSGLTPDRVDALLGLGGLRYLSVNISTLEKDRYARDRGVDQLET
                     VVRNLDYMRDKPVAEEMDLVVLGGGDEPHQKAHREITERYGGSRFNVRPFEARDRAGW
                     LPTGVRPAEKHRKLRGCDNVGSRPLEHLHVTAEGKCIFCCEDYDEKLVVGDLKTQSVR
                     EVLEGPALALLRRWSYGVEEAPDDFICRSCIAARTEGE"
     gene            19569..21488
                     /locus_tag="P5164_00220"
     CDS             19569..21488
                     /locus_tag="P5164_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42340.1"
                     /translation="MPSRPAPALPRPARGALLAVAAALLLTADDRHAGKVSDGRQTIG
                     TAVAIVETGELGIGKGAPRAFEREADAVSRYGLGASLAQLPAAAAAPAVEGALGPGTS
                     QPLFLLAPLAGVLLAAAAAGGIARLAGASTSLQGLAILLASLGGPLGSYATSDFSEAL
                     QAAALAGALLLALGAVREDDPRVSRRRAFGAGLAAGAALLVKSSLAAVAPILLLPLFA
                     ASALRGRRLAAAAAGAALPVAAWVAFELHRFGRLFSGYEGEGFTHPLPEGLWRLLVGP
                     NRGLVLFFPAALLAAAALSRALRRPGDAPLCLAAAGSVLAFLVLLATAAAWWAWHGVG
                     GWGPRLLIPAVPLLAPWAALAAEGLGTGGRRAVAVLSVALNLPPLLVHPSLVDTYVAN
                     CRRAPLTPELERVVPPLAVGPDDAGRPSVSPENVLSKEPLASPFLLYPWYAAASAPRS
                     AESRAERLARPPWYERRPELGPRLVPFPPELADVVAPPLGWSFFGRALGRGIADPASF
                     AVYLASLSEQVGRAHEAGRLERALELARKLARLDPGEGSDALLLESLRLLRREETLRA
                     ALAALPEERAAAPAVLVVRALAARDAGDLALAARLGEAAAPFFPGTALGRDGARPASW
                     PATFAALTRPLVPSA"
     gene            21541..>22010
                     /locus_tag="P5164_00225"
     CDS             21541..>22010
                     /locus_tag="P5164_00225"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRY42341.1"
                     /translation="MTTSQHKNMAILVGGGPAPGINSVISAATIRAELEGVSVLGIRD
                     GFQWLMQGNIEHVHPLTIEEVSRIHFRGGSHIGIARANPTKDPQLLENAVISLLRLNV
                     SRLVTIGGDDTAFSAMKLAEKADGRIAVVHVPKTIDNDLDLPPDVDTFGFQTAR"
     assembly_gap    22011..22110
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <22111..22749
                     /locus_tag="P5164_00230"
     CDS             <22111..22749
                     /locus_tag="P5164_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRY42342.1"
                     /translation="DGVAIIAEGLVLELDPEELKGLAEAERDAHGHLRIADVNIGEIL
                     KMAVQEELKKLGVKATVTDKNIGYELRCADPIPFDLEYTRDLGYCAAKYILSGGTGAM
                     VSMQGGNFVAIPFEQMLDPATGRTKVRMVDVHSTRYAIARRYMIRLRKDDFEDPHELA
                     KFAATCGMSLADFRQRFGYLVENELPPIVLLPSGHRDETGPGSGSYFEGETL"
     gene            22854..23936
                     /locus_tag="P5164_00235"
     CDS             22854..23936
                     /locus_tag="P5164_00235"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRY42343.1"
                     /translation="MAEIRWCTGETGRSEPLGGDGPLGLGREPDNHFVLADPGVSRHH
                     ARLVRSGDDWAIEDLGSSNGTCVNGRRVSRALLKDGDRVRLCGVELEFRDVPAAGTPP
                     DAADGDASYFLARGGQRLGPYAWEDLTGFAANGQVAPDDELWGPGLDRWTPATAIPGL
                     LPPVPAPLPRPTPKRPETSARRRSPFARGLLVAAPAAALLLLLGALGWWFWGSHPGAD
                     GGDAGDGNRRAIETATAQAPPAPPPPVDDWKEYPDLEAERRRIGDSLSAFQRALRAGA
                     VDEAVSWIATERQATYAALFRNKPAAMASFADLLERSQLTFLAPPAEPSVATTVRTAE
                     YTVTIDGFDFYLRWCRRDDRWVLFDF"
     gene            23915..26467
                     /locus_tag="P5164_00240"
     CDS             23915..26467
                     /locus_tag="P5164_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42344.1"
                     /translation="MGPLRLLRGARTARVASGALALTILGVVTPLRGWTQAPHRQINA
                     EAVAAFFAKMEREPSEKFRLGPVPTDERKALHRGIAVTSSTMFVKPSGGLAEAYEIGE
                     ARHAMPAWISLGGDWADEPHLYASVRHFYDPLQVAGVPFLTDQLWAHGTYDSPGIEAV
                     TWALDHPDNPFSLRQGMVAYKLAMETPEDVPPPPGLRESHFKTNVSLVPGNAAEQRSL
                     YLARAYRALGEAMHMIGDMTQPAHVRNDSHPMDEPIESAVTTSDVRLAAASPVIDAAL
                     RPYLSSAGGTLQAPRALSRQVALFTNRTFYSMDTIFDRRTGTLPTNGMPPYPSPQFGD
                     LVVRTATVRGFVGKRAVTKLLGRVAGREVPMAQERLSFHWFDPDHSVIFGPGLETASG
                     FTSKLGPYMVPQAFAREQAAALLPVAIHACADLMDLFFPTLELKAEYRLEGPGPDGSS
                     GRQVVRIEPGMVHLRERDPAWSAFDLDVAYTGPGALVITRGGKVEATRKLHFRKGLLD
                     TIENVAGKMVKAPLRVFVSDGRTALTGEEELYSFAHGQKLHVEIDSGSRHFESPPYEL
                     RHEVVIQPRRGVGPPGATFDFEAHARPEGVYRFEWSWPGAGEVVATEGSTSKVAPVLV
                     REGEYPFTVKLVHPDGTVLAEDRATAAVEKETAAPPTPVAKKGPGRWVLVRQWNDVWK
                     DPRVTSSRATAEAGVNSAKIHVTVPDRQTTWQFDYAMSWSPLPASVGRDETFDVTLRL
                     DSIRVPEPRVGWGAEIAVSTGRTEAIRATAPPKAANFVSQTTWNGSVLQGGLGSAARK
                     PPDSRSVTLDLGWRSPEDGDVQVVTVRTWGQGAGAGGNGAAIHFHYEYRFQE"
     gene            complement(26594..28831)
                     /locus_tag="P5164_00245"
     CDS             complement(26594..28831)
                     /locus_tag="P5164_00245"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRY42345.1"
                     /translation="MPSESPRRVAILTSGGDAPGMNAVVRAAVRAALEKGLEVLAVEE
                     GFQGLVDGGERFRRMAWGDVGGILPKGGTVIGTARCPAFRTREGRLAAARNLARERVE
                     ALIVVGGDGSLTGADLFRREWPSLLKELAAGGEIPPEAARSASPLALVGLVGSIDNDM
                     AGTDMTIGADTALHRIVEAVDALVSTAASHQRTFVVEVMGRHCGYLALMSALATGAGW
                     VFIPEAPPEGDDWEEKLCAVLAEGRKAGRRHSTVILAEGAVDRQGRPIEAERIRKLLE
                     ERLGTETRTTVLGHVQRGGAPSAFDRTMGTLLGVAAIEEVVRWGPDDVPCLIGLRENR
                     VTRVPLLENVEKARAVGEAIRSGDFERAMTLRGTSFRSSFRIMKTLVRAFPHGPRDGQ
                     RRRRFLVLHAGPPAPGMNTAVRAAVRLLVDQGHVVLGARGGFDGLLAGDVVPLDWMSV
                     NGWVSLGGAELGTSRRVPAAEDLGALAEVLAARQVEGVLLVGGWAAYQAAGRMQSARA
                     EHAAFRVPLVCVPAGIDNSLPGSELAIGADTALNAIVEAVDRIKQSAVAWKRTFVVEV
                     MGRWCGYLAVLSGLATGAERVFTHEEGITMKALERDVASLVEGFRKGKRLALMIRNEN
                     ASPVFTTDFLRRLFEAEGRGAFDARQAILGHLQQGGDPTPFDRIVATRYAARAVEVLL
                     EESAKESPAAVFLGVQSGRLTAFPLDALASMVEPEFERAREPWWRSIEPVVRLLGQPG
                     PSAKA"
     gene            complement(28877..32326)
                     /locus_tag="P5164_00250"
     CDS             complement(28877..32326)
                     /locus_tag="P5164_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRY42346.1"
                     /translation="MRTDEPADGGGSERYRRQRRIQEATYAIAHAVTTTAGLDDLFAR
                     IHRIVGGLMEARNLYIALLDEATGSLSFPYFRDEIDAEPPEGPVPAGRGLTGYVLRTG
                     EPLLASPEVFDALVARGEVAPIGAASIDWLGVPLRAGERTFGVLAVQSYAGNVRYGEE
                     DQALLGFVSSQVALAIERRRAEEALRLSEMRHRAVLEALPDLLFVLDREGRYLAVHAA
                     RPEALAAPRESLLGSSVPEVLPKEAADVWMDAIRRCLGGGGMQVIEYELDLQAGRRAF
                     EGRVVPHDADSVLSIVRDVTDRVERERELRERERELKRLTENMIDVITETDLHGNLRF
                     ATPSYVAATGWSPEEFLGHNALEFVHPEDAELSREMLGRRARRDGASAFTYRFRTKDG
                     RWLWVDSIVTLAKGERGESDGLVVTSRDVTGRKRDEEVLRLQHETERGILRREEPTVI
                     LARICTRVSELFDLPAVWIGLKQPDGRIEPRAVAGSASGFVARSTFRWDGSPEGRGPS
                     GEAVRTGRPVFVDGLGDDRVARWRDAAAATGLGAALSVPLVVGDRVLGVLSCYARGPE
                     AFAGAAVVPIARVADQVALSLLEAEQLERIELQTAALEATANAVVITDREGRVEWVNR
                     AFTELTGFSAEELRGETPRVLKSGIQSEFFYERMWETILEGNVWRGELYNRRKDGTVY
                     VEEQTITPVRGADGGIRHFVAVKQDVTQRRRTEERIRYLALHDPLTDLGNRRAAEESL
                     ERIVARARRGTPGSLLLLDLDHFKVVNDTLGHGAGDAVLVELARLLGTLRRPGDEMAR
                     LGGDEFVLVLEGIPAEAGRMVAERLRRAVHEHRFEVEGRRFDLGVSVGVVPIDGRLKP
                     PALLALADSALYAAKERGRNRVVLFDGSVAPTTLSEASLWASRVKDALREGRFVLVYQ
                     PILRLESGRTAHYEALLRLSDGDGTLVAPGDFLPAAEQFGLLPQIDAWVVDEVLSVLR
                     TREDVEVFVNLSGASLGEEGLLATLEERIRESGIGPGRLAFELTETTAVRDLIGAREW
                     MRRLRDLGCRFALDDFGIGFSSFSYLQGLPADYVKIDGSFIRDLETNPSNRALVKAID
                     TVAHTLGKETIAEHVESLDAVAILRELGVEYGQGFALGAPKRELPGLPDARDAATSPG
                     TLR"
     gene            32540..>33768
                     /locus_tag="P5164_00255"
     CDS             32540..>33768
                     /locus_tag="P5164_00255"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRY42347.1"
                     /translation="MRYGVAAAVLGLLASGAVRADEPLTLGEAMARARDGAREVAAAT
                     ARKEAASARARQASAFRLPSLTLSETYVRTSSPVDSFGLELNREEFTFQKFVATDPND
                     PGWSGTAITRLEAQAPIFTGGELSGRIAQARSAADAAASQASWAADNAALAAGEAWVM
                     LAQAEEFVALLTKARDTVAAHVALARSYVEQGMLVRSELLRAEVELARVEDLLEDAKG
                     KARVANANLAFRIGVPQDSTWKLAPLPGPGPLDGDASHWVSSADARKDLSAARSLLRA
                     GELEEKVKKAAWLPKVGVLARWDLHDKNLFGASGSAGTVLAFASLNLFQGGADKAALV
                     AAREEARAGREDVARFADGVALEVRQAYEEAATARARHATAVKALEAARESERITDER
                     FRSGVVKTIDLLDAATAR"
BASE COUNT         4536 a        12687 c        12052 g         4393 t
ORIGIN      
        1 aggatcgcgt agacgatcga cttgccgacg atgacctgga cgtcgaagag gcggaaccgg
       61 acgatcgcgt aggcgaacgt gagcggcacg agcgccatcg ggacgacgcc ccaggcgagg
      121 tagcgctcgt cccggaagag cgacgggaag tagaccgcga agaggacgaa cgggacgatc
      181 ccgccgaggg tgccgagcag gaggaccagg atcggccgcc gcgtcgtctt ctcgcgcgcc
      241 gtccaccagg agtgggcgag cgcgaggagc ccgaggacga ggtagtcggc gaggaggatc
      301 cagttcgccc ggggggcgcc gtagacgagc gcggagcggt cgcccgtcct ccggaggacc
      361 gccatctgga gggcgtagaa cgccggcggg agcgtgtaga ggatgacgaa gagggcgggc
      421 gacccgttca ggaaccgctg gaggaggacg agcggcgccg ccggcggctc ctctcccggg
      481 cgcgcctccg cgaaccggaa gaccttccgg cgcgggaaca ggaggaagaa gtggaggaag
      541 accgcgccga ggaggaagag ggccagcgtc cccgccacct gcacgaacca gtcgatccag
      601 tagtagctgg acggccgcag gcggcagacg aagaagagca tgaagaggac gcagaggaga
      661 aagaagacct ccgccgcctc gtctccggcc cgccgcgagt agacgaagag gccgaggccg
      721 aagaagagcc cgccgaggag ggcgtagtag acgtacgacg ccgacccgag ccggtagggg
      781 gagaggagga cctcgcgctc gaggacctgc tctccccgct tgatcaggta cgggacggtc
      841 tctcccgccg cctgccggcc gagctcggag gccgcctcgg ccggcgtgtt cagcatccgc
      901 cggccgatcc cgaggatcgt gtcgcccggg cggatgcccg ccgcctccgc gggcccgccc
      961 cggacgacgg cgcggacctg gagcccgctt tcggcgtagg tgtccgggac gacgccgtcc
     1021 cacgccttcg gaaggaacat gtcggccacc gacatgagcg cgatcagcgc gccgacgacc
     1081 ccgacgacga cgatggccct ccggcgggct gcgggcggcg cgggggtcac ggcactcttc
     1141 tctcgatcgg ctctcgcgtc gggggcgcac gaagtatacg gaggccgccc cggtcggttc
     1201 cgagacccgt gccgcctgca tgcgagaatc gcgcctcgtg accgcccgca gcaggagggg
     1261 cctcttcgtc accttcgagg ggatcgaggg ctccggcaag tcgacccagc tccgcattct
     1321 cgcccggcgt ctccgccggc tcggcgtgcc gctcctcgtg acgcgccagc cgggcggcac
     1381 gccggccggg gctcgccttc ggcggctcct cctccacgcc gggaagctcg acccgcgggc
     1441 cgagctcctc ctgatgttcg cggaccggcg gcagcacctg acggagtcga tcgaaccggc
     1501 gctctccgcc ggaacgctcg tcctctgcga ccgctatacc gacgcctcac gcgcctacca
     1561 gggggcgggg cgcgacctcg gagaggagct cgtcgacctc ctccacgcgc gtttctgcgg
     1621 gctcgagccc gaccggacgt acctcttcga ctgcccgccc gcgacggggc tcgcccgagt
     1681 cgccgccgcg cgcgggggga aggaccggat cgagcgcgag gcgctcgcct tccacaggcg
     1741 cgtccgggcc gcctacctcg cccgggcgcg gagggagccc gaccgcttcc tcgtcctcga
     1801 cgggacccgc cccgtcgagg cccttgcgga gcaggtcgcc ggcgacctcg aggcgctccg
     1861 ggcggcgcga tgaccgacgt tcccgtccag ctcctgaccg ggcgcgggtc gcggcgcctc
     1921 gaagcggagg cgctcgacgt ggccgccgcc ctcctctgcc ggagcggcga ccggacgatc
     1981 gaatgccccg actgccgccg cgtcgcccgc cgcgagcacc ccgacctcct cgtcgccgcc
     2041 cccgagaggg cgcgccgcgc gaacacgcct ccgttcgtcg agtcctccga gtcgaaggag
     2101 acgacgatcc cgacggcgct cgtccgcgca atctcggccg acgcctcgcg cctgccgtac
     2161 gaggggacgc gccgcgtgct cgtcctcctc gacgtcgacc ggacggagtc ggccgcgttc
     2221 agcgcgctcc tgaaggtcct ggaggagccg ccgtcgcgcg cacggttcgt cctgaccgcc
     2281 acgcgccccc gcctcctccc gccgacgatc ctctcgcggc tcgtcctccg caggctcccc
     2341 gccttctccc gggaggcgct cgtcgccgcc ctcgtcgccg gcggaatgcc gcccgaggat
     2401 gcgtccgcgc gcgcggcctt cggccctccg ggcgaggagg aggcgcgcga cctggacctc
     2461 ggcgccgcgc gggagacgcg cgacgccctg ctggaggcgg tctcggggct cctcctgtcg
     2521 cgctccacgg gctgggcgct cgtcctcgcc ggccggctcg ccgccgagga cgccggggcg
     2581 accgcggacc gcctctcgct cctcgcacgc ctcctcaggg acgcggtcgc cgccggcacc
     2641 gaccccgccg gcaggaccgt ccatcacgtc gagcgctacc gggacctcgc ccggctctcc
     2701 gaagcggggg tcgacgtcct gctcgaagcg gcgcgccggg cgctccgcga ggccggcgac
     2761 cttcccgact cgcgccggaa cccgcgcctg gccgccgagg ccttcgcgct ctcgctgcgg
     2821 gcgctctgag acgcgggccg ggcggggcgc ccccgaaggc gcgccccgcc cgggcggcga
     2881 tgcggtccgc tacttctcga gctcgacgac gacgtcgatc gccgctcccg gcttcgcctc
     2941 gacctcgatc gtcttcgtga cgaaacccgg gcggacgacc gagacggtgt gcctgccggc
     3001 atcggccgga acgccccggc ggagcccggc gagctccttg ccggtgccga tgtattcgtc
     3061 gtcgaggtag accgccgcgt cggccggctg gatctcgaag cgcagccggc ctcgctcggc
     3121 ggggagcgcc tcgcgagcgg agcgcccttc cttcgcgtcg gtgcggggct tcggcgccgg
     3181 ggcgatgtcc tcgtccgcct ccttcagctc ctcggcgagc tcctcgagct catcgaggtc
     3241 gtcgagctct tcccggtcgc gctcgcgatc ggcctcggct cgcgcctcga agcgtcccgt
     3301 ccggtcccgg cggggctcct cgggcttgcc gaagacccgc ccgtggggcg tgccccggcg
     3361 cgggggctcg gtcgcctcga gccgcttctt gccgggctcg agcttcaggt cgtcccggaa
     3421 gcgaagggcc tgtccgggcc gaacctcgac ctcgtgcttg agggtctcgt acgaggggtg
     3481 gcggaactcg atcgtgtact tgccggcctc cagccagagg aagtcgggat agccgtcgaa
     3541 gtcgtcggcc gagccgatga acgtgccgtc gaggtagacc tcggccgctt ccgggctgac
     3601 gtcggtgtcg acccgggcgt agcggtacgg gtcggggctg tagtcctcga cgatgacgac
     3661 accgccgaag ccccagtggt acgggccgaa gcggctcccc caccaccacc agtcccaggg
     3721 accgccccac caccacgacg tcccgcccca accggtgtac ggcgagtagt accggcgccg
     3781 cgggtgccgg tgcgacccgg agcccggccc gcgtggatgc gccgcgacga ccccggggac
     3841 cctcccgccc ccgccgccgg cggtcgcgga cgtcgagccg ccgctccgtc cgtcgcgcgg
     3901 ctcggcggcg acgacgcccc gtcccggacg cgccggcgct cccgcgctcg tctccctcgg
     3961 ggcggccgcg cggcgcggcg tcgaaggaga gcccgccgag ggcgccggag cgctccgcgg
     4021 ggccgaaggc gccggcgccg agtagctcgg cgacggggag gagcgggacg gcgcggaggc
     4081 cgaggaggac ggagccggcg cggaccccgt gctcggcgag gccgtccccg gctcgcgctg
     4141 ggcgaaggcg gtcggcgcga gaaggagagc cggcagcgcg acgagcgcgc cggcgaggcc
     4201 cgggcgcgac caggtcgccg tccgggagga tgtgcgggag agcttcggat cggtcatggc
     4261 ggcctccgct gcggcccgtc ctccccgagg gaccggcgga ccgctcgacg agtcagaagc
     4321 aatctccctg ccgtccacgc ccgtcccgcc ggcgagacgg cccctccggc ggaggggtca
     4381 gcggccggag tggaaggccc aggccacgag cgcgtggtag acgcgcagcg ggagcggcac
     4441 cccggccgag tcgaggcgcg cggccacccg ccccagcgct cgataggcga acgagaaggc
     4501 ccggtggcgg aagcgcgggc gcggcggcgg aagccagcgg ttcccctcga gctccgggtg
     4561 gatctcgtag aggacgtcga tcgcccggcc aacgtgcgcc tggcgcgcga ggaactgcgc
     4621 catcgtctgc gggtggacgt gccgggtggc ggcggccttc cggtagacga tggggagccc
     4681 cgccgaggaa aggcgccagc cgagctcgca gtcctcccag ccgtacgacg tgaagcggtg
     4741 gtcgaagggc tcctcgccga ggaggtcgcg ggggatcgag acgttcgagg tgtagagcgt
     4801 cgtgaacggg acctcctggc cgtcgcggag gaggtcgaac gagaactgcg ggccgtggcc
     4861 gttgacgaag tcgaggaacg gcgtcacccg catcctctcc cggtgccagc cggtgaagcc
     4921 gatgacggcg caggcgccgg gacgggacgc gcgcgcggcg aggtgctcgg cgacgcagga
     4981 gggctcgggg atcgtgtcgt ctcccaggaa gaggacgagc gcccccctcg cgtgggcgac
     5041 gccgaggttc cgcgcccgtc cctgcttcag gttcggctgg cggagcggga cgaggtcgag
     5101 ccggtcggcc agctcctccc ggagccgatg gagcagctcc gtcgtcccgt ccttcgagcc
     5161 gtcgtcgacg acgatcacct cgaggggccg gacggtctgc gacgccagcg cgcggacggt
     5221 ctcctcgagg accgccttcc gctcgtacgt cgggatgacg acggagaccg acgcgagggg
     5281 gtgccgttcc tcgggcgccg cgggaacgaa cgcgacgctc cggacgcccg cgccgagcct
     5341 ccggaagtcc cagctcccct gtgcgtcgtc cggaacgccg gacggcgatc gaagaacgac
     5401 gtcgaggaat ccgtccgcgg cggcggagac cgggacctcc aggttgcgcc acgcgccggg
     5461 ctcgagctcg gccgtcgtct cccgcccgtt gcacgtcacc cggagcgggc ccgcgggcgc
     5521 cgccgggctg ccgagggcga cgatcttcag ccgcccctcc gcgcccggcg cgacccgcag
     5581 ccccagccgg gcgtcgccgg cggtccagcg gaacgaggcc gtcccgtcgt gctccggccc
     5641 gtaccagccc cagccgagac ggctctcgcc ggaaggctcg cccgggagga ggacgttcga
     5701 gacgatccag gcgtcgcacc ggacctcgcg gaagcgcggc gcctccggcg gcggtggcgg
     5761 ctgcagcgcc cgaaggacga ggcccgcgcg gcggagctct tccgaggacc gcttgcgcct
     5821 cgcgttcgtc tcggcgcgga ggcggagcgt ccccggcagg tggcggaggt tccagaggac
     5881 ggcgtgcgcg aggaggcgcc aggcacccgc ccgcgcgagg agcaccgcgt gccaccgaag
     5941 gaccttcgcc gcctcgaccg gctcgacgta cttcaggatc gagcggacgg cgtggcggac
     6001 cccgagcttc agcctccagt cgaggccccg gacggccttc gaggtcccga ggaaggcgtg
     6061 cctcacgacc gagtcccggc agacgacgac gcgccggccg aggagccaga gcctccagcc
     6121 gaggtcgacg tcctcgtggt acatgaagaa cgccgggtcg aagccgccga ggccgaagaa
     6181 ctcgtgccgc cgcatcgcca tcgccgcggc cgtcgggaag aggacgtcgt gccggcgcgg
     6241 ctcctccccc gacgggtccc atggcgcgta cgggaagcgg tacccgtggt cgtacgcgta
     6301 ggcgaggcgg gtcatcccgc cgccgaggcc gttcaggagc tccggcgtcg ccagcagccg
     6361 gagcgtcgag caggtcgccg cgatctcggg ctcggcgtcg aggggcgcga ggagccccgc
     6421 gagccagccc tcctcgacga cggtgtccga gttcaggagg acgaggaagt cgccgcgcgc
     6481 ctcgagcgcg ccgcgccggt tcgcctcgcc gaagccgagg ttcgccccca tcggcagcac
     6541 cttcacggcc gggtgccgct cgcggaccca cgcgacgctg tcgtcggagg agccgttgtc
     6601 gacgaggatc acctctcccg gcttcgggtc cgacgcctcg agggcgggaa ggcacttctc
     6661 gaggtggtgc cgcccgtcga agttgacgac gacgaccgag gcccgcgtcg cgagcgtccg
     6721 tcccgcgcgc cgttccgcgt cggcccgctc cgcctcgagg cggtccctct cgcggaccga
     6781 ctcctccgtc gcgcgagaca ggagcgtctc gaccgcggcg acctcccggc ggacctccct
     6841 cggcaggagg tccttcttga cgtggaggag gatcgccgag agcgcctccg gcccgtagcg
     6901 gccggcgtgc ttgcgcagga cggcgacgcg gtccctctcc tgcgtcgtcg cggcgtcggg
     6961 gtcgtcgacg aagtgccccg ggaagacgcg gtactccgag acggggacgt cgacgagccg
     7021 gaagcggtgc gtctgcgaga ggcggaggaa gaggtcccag tcctcgaatc tctccagcgc
     7081 cgcgtcgagc ccgccggccg cgagcagcgc ctcccgcggg agcagcggcg cgctcatcgg
     7141 gatgaagttc tccaggagga gcgccgcgtc gtcgaacggc tcggcgaagg aacggaacgg
     7201 cgtccggagc cgtccctccc ctgtcccgct ccagcggaac gcctcgacgc gcccgtagac
     7261 gacctcggcc tcccgcgcct ccgcggcgag cgccgggagc ccgcccggga ggaggaggtc
     7321 gtcgtcgtcg aggaagtgga cgaaccgtcc ggtcgcggcc tcgacccccg cgttcgccgc
     7381 cgccgcccgg ccgcgccccg gtcgcaggtc gacgagccga aggcggagac ccgggaagcg
     7441 cgcgaggacg cccgagacgt cggcgccgcc gtcgttcacg acgaccgcct cgagcggcac
     7501 gcccgtttcc gccgcgacgc tcgcgagcgc ctcggcgagg agctccggtc ggtccttcgt
     7561 ccggaggacg acgctgacga gcggcgtgcc gtctcccctc ccgaccggaa gaccggcggc
     7621 cggttcgacc gtcggcagct ccggggaggt ggccgggaag agctcccccg gcgacagcgt
     7681 tgggccgatc gcccgcgcga gacggtgaac gtcgatggcg ggcagctccg cgagcgccac
     7741 gaggaagaac ccctcggccg acgccacgct ccggggaagg ctcatcctcc ggtactgccc
     7801 cagcccccgc gcaaaggccg cgtagtcgtg gccgtcgagc tgggagacga acgccgcgac
     7861 cgcggcctcc ttgcgggcga tcgtcatcga gacgtcgacg aggacgttcg ggcggaacgg
     7921 ctgggagacc tcgaagaagg ccgccctggc cccctcggga agcgccgcga cgaggcggcg
     7981 cccggcgtcg cccgcgaaga gggagaggaa cgcctccgcg acggctcggt gatcggggtg
     8041 aacctccacg ggcgagggga cgaagacgag gtcgggaagg gccgcgagga ggccgctctc
     8101 gatcgccgcc gcgagagggt cgcccgtgcg cccgagccca cggtcgggaa ggaatgcgca
     8161 gcgcgccgcg ccgccgccga gccggcggag cgcctcgcgc gtctcctcca ggcgccgcgc
     8221 gccgacgagc cgccgtgcct cggcgtccgg ctcgtccccg gcgccgtcgg tgacgacgag
     8281 gacgtcgacc cgcgcgccgc cgtcgacgag cgcggcgagc gtcccgccgc agccgaacgt
     8341 ctcgtcgtcg gcgtgcggcg ccaggacgag gactcgccgc gcctcgaggc gggtcgtctc
     8401 gtacgggagg agtcgtcgct cggggaaggg gtccggggag gggggagcca cgcggcgcga
     8461 ttctaccggc cgggcgcccg ggagagccgc cgcgtcagcc ggcgaggcgg gcctcggcga
     8521 gggtcgagcc cgtcgtccgc gcgaacgcgc ggacgaagag cgcctcgccg gcgtgcgccg
     8581 ccacgaggtc ggccgggggc gtccagcgtc tctcgccggg cggcacgagc gcgagcgcga
     8641 acggccggaa gtgccggaac ggcgagatcg cgaccgccgc gagggccgcg tcgtcccagg
     8701 agaggccgtc cctgacgggg agcggcggcg cctcgaacgg cgcgcaggat gcgaggcggg
     8761 cgaggcccct ccgccccgtc tcgccgaagt gcttcgcgta gtagcgctgc tcggactccg
     8821 cgaaccaggc ggcgacccgc ggctcggtct tcgtgctctg cgcgaagagg tggacgacgt
     8881 gcgcgccgcc gacgtagacg agccctcccc cgcaggccag gagccttctc tgccagtcgt
     8941 tctcctcgta gtagagcggg tacgcctcgt cgaacggccc gaccgcctcg agcgcggcgc
     9001 gcgtgaccgc gacgagcgcc cccgagaggc cgcggacggc agcgctctcg ccggccgcgg
     9061 cgcgctccgc ctggaccgcc gcccgccgcg cgtcgcgccg gaagacacgg gcggcccggg
     9121 ccgggtcggc cgcgagcgtt ctccgcgcga gctcctccgg gcgcccctcc tccgccgggg
     9181 gaaggagaac gcttcgcgcg ccgtcggcgt agagcgccgg cccggctgcg aggatgcccc
     9241 cggcctcggc cgcgtcgacg agggccgcga cggagccctc gcaaaagaga aggtcggggt
     9301 tcgcgaggag gagcgtgccg ccccgagccg ccgcgatgcc ggcgttcagc cctccggcga
     9361 agccgaggtt ccgcggaggg aggaggacgc ggtcggcgtg ggggacgagg gcgtcccgct
     9421 ccgcgccgtc gcccgagttc accacgacga ccacctcggc ctcccgcccc gtccggcggg
     9481 cctccccgcg gaacgtcgcg atcgcgcgct cggcgagcgc cgcggatcgg taggagacga
     9541 agacggccga gatcatcggg tgctaccctt ccgcgcgccg tggcgatcga gttccgaccg
     9601 gccgggaaga gcgatcgggc tgccatcatg gagctcttcg aggtggcgtt caaggcgccg
     9661 gcgagcgccg ggcttctcga ccggcgggcc gtcttcacgt cggacgccgc tcgagtcgcg
     9721 acgcgcggct cctgatggct ttccccgacc acggccgacc ggctcccgca ccggcgagcg
     9781 gattcgctct cctcgcgctg atctccacgg ccggtctcgc gctgcggctc gggttcgtcc
     9841 acgccatcgg ctttcagccc ggcttcttcg acgtggacgg ctaccacgcg atggccctcg
     9901 cagcggcgaa cggggcctcc gttccgacgt cccatcaccc tcccggctac gcgtggttcc
     9961 tctggctcgt ctacgaagcg ctcggcgtcc ggccccggat cgtctacctc ctgcaggccg
    10021 tcctcggcgc gggggccgct cttctcgccg gagactcgat tcgccgggtc gccggtacca
    10081 gtgccggcgc cctcgccgcc gccctcctcg ccttcaacgc ctacgccgtc ctcgcgacct
    10141 cgactctcgc cagcgagaac ctcgcgctgc cgggagtcgc gctggttctc tggctctccc
    10201 tcgacgggtc gcgcagaccc gcggcctgga ggctgctctc cgcggcgctc gtcgtctcgg
    10261 gcctcgcgct cgtccgaacg ggactggtgc tcctcgccgt tccgctcgcc gtcctcgtcc
    10321 tccttccccg gcccgctcgc ggcgggtccc tctcccggcg cctcgctgcc tcgctcggcg
    10381 tcgtcctcgt cgccttctcg ggactgttcc ttcacgcggt ctcgcgatgg aggacgacgg
    10441 gggtcttccg gatcggctgt ccgaacgacg tctacaacct ctgggtcggc aacaacccgg
    10501 acgcgaccgg gcggatcgac ccgatgcccg tcgtcccggg cgtcgacctc gacaaggagg
    10561 cccaggcgag actcctcgcg ccgcgcgtcc gggcgttcct ccgggaccat cctctccagc
    10621 aggtgcgcct cttcctcgcc cgcgcctcct acaacttcgc tccgcccaaa cgggacctga
    10681 tctacgtcta cgggcacggc tgggccgggg agagaccgcg atccgtggtc cttgcgacct
    10741 acgcgacggt cgcgctctcc ctgccgatcc tgctcgcggt cgccctcctg gctttcgcgc
    10801 gttccggaca cctgccggcc ttccgcctgg cggcggcgat gaccgccgcg gtcctcctcc
    10861 cgtaccaggt ctcgatcggc gacccgcgtt tcctcctccc ggcctatccc gccctctgcg
    10921 cggcggcggg tctcctcggt gcgtcccgag tcccggcggc gtggagtcgc gggcggagga
    10981 tcgtcgccgc cctcgcgatg gctctcttct gcgcgaacgc ggctcgggac ctgtcgctta
    11041 ccgaccccgc tctccgcgcg gtgtccggcc cgggaggctc gcggctgcgc ccgccgtacc
    11101 acttcgcccg ctgagacgcg cggccggcgc ccccccgctc tacctcgtgg tcgaagggcc
    11161 ccgcgcggcc ggcctcccca cccggaacag gaggtactcc ggcccgacct cctccacggc
    11221 ggctgcgcag ccgcgcacgg ccagggccgg gccgtaggac ctcctcaggc tctcgaacgt
    11281 tgcgtgagtc tcgcggttcc gcatcagggc ctcgagggaa tcccagacgt agacggagac
    11341 gaagacggaa tcgtaggagc agatcctctc ggccgtcacg ggggcctcga gggcgtcgat
    11401 cgcgagctgg gcgcgctggt ccggatcgag ccagagcgga aacgtcaggg ccgcgacgcg
    11461 accgaggtcc ccgcgccgga cccgctcgaa catccgtcct tgtcccgcgc cccagtcgac
    11521 gttcgagtcc gagagctttc cacggaggcc gcgggctcca ccggcgatca cgttcccgaa
    11581 gctcgtctcc cgcccccagg ccggcccggc ctcgaccgcc gccaggagcg ccgtcgctgc
    11641 gaggaggagc gcgcgagcgc gaggccggaa gggcgagacc gcgcgcagcc cgatcaggac
    11701 cccgacgacg agaagcgccg tggccggcat cacgtgacgg accccgatgt tcagcctcgc
    11761 cgccatcgcg aggacgaaat aggccaggcc gagcgtcagg gggacgaagg tccgggcgca
    11821 ggcgacgccc acgaggcggc tccggccccg ccggagggcc cggaccacga gtcccggcgc
    11881 cgccagaagc gccaggccga agaaggccac ggtggccgtc gtcatcttga gggcgagcgc
    11941 gacggggaaa tacaggagga acccgtctcc ggagacctcc ccgaacaggt agttcagccg
    12001 accgccggga cggcttccct tctcgaccca ctcgagcccc gctgcgtacg ccgcgagtcc
    12061 ccgcggcagc cgttccgccg ccgtggagag gagctccgtc gcggaggtcc tcgggtggaa
    12121 tccgcggtcc gcgatgctcc gccggatcgc cgcgcgcaga gcctcgggcg gcgtcggccc
    12181 ggtcacggcc tgctgcacgg cgacgagagt cgcgagggcc ggcgtcacga cggtcactgc
    12241 cgtcagggcg gcggcacggg ccagagggcc ggctcggagc gcccgcgaga cgggcgaccg
    12301 gcccgcggcg gcgacgcgaa gccaggccac gccggcgaac gccgccagga aagggagagc
    12361 ggagtacttc gacacgagcg ccccgccgta gaggagtccc gagaggacga ggccgacgac
    12421 cgagacgcgg cggcctcgcg tggaccggtc gagggccgca gccgccgaca cgaggaagaa
    12481 cgtcaggagg acgtcggtgt ggacgattcc cgagctcgag cggaacgacg gctcgatcag
    12541 gagaattccc gcggcgaacg ccccgcacgc cgctccggct ctcgcgcgga cccacagtcc
    12601 cgtcacgagg aggagcgcgc cgaagacgac ggggaagagc ctccgggccc tgcggacggt
    12661 ctcgatggcg gtccccgcgg gcgccgcccc gagaccggcc gcgccgagga ccttcatcag
    12721 gacggggtgc tcgaaattcg agacgacgcc tcgtccctcg cgcagctccc gggaggcctt
    12781 gacgtgcgcc ggctcgtcga tcgtccacca cgtgcttcgg cgcgagtgct cgccggcgac
    12841 ccagccggcg atcgcgacga gggcgacggc gatcaggccg gcggcgcgcc ttcgcggacg
    12901 tctcccgcgc cccccagcgg gcgccggtgc gcacccgtcg cgagcctcgg ggccgccggc
    12961 ttcatcggcc ggcggacccg cgtccctcct cggctcgccc gaacggagcg actccggagg
    13021 ctgaggcggc gtcgacgttc tcgagccgga gagggcagcg gtcgagatca tcgggtggta
    13081 cccttccgcg cgccgtggcg atcgaattcc gaccggccgg gaagagcgat cgggccgcca
    13141 tcctggagct tttcgaggtg gcgttcaagg cgccggcgaa cgccgccgac ctcgcttgga
    13201 agtacgacgt caacccccac ccggctctca gcgccctcgc gatcgaggac ggccgtgcgc
    13261 tcggcttcat cggggccctc gggacgcgct accggggcga ggggatggac cagcagggga
    13321 acaccgtcgt cgacgtcatg acgcgccccg actcgcgcgc gctcggacgg gccgggatgt
    13381 tcaagcgcct cggcgagctc ttccggcgga tcaacgcgga ggccgggacg ctcttcgact
    13441 tcgggttccc gaacgagcgc gcgaggaaga tcgaggagcg ccttttcggc tgcgtcctgg
    13501 ccgagccgtg cgcggagcgg ggccgccggc tcgacgcacc gccgctcctc ggacggctgc
    13561 ggcgccggct cctccgcgtg agcgaggggg agccgttcgg acgcgcgcac gagccgctcg
    13621 ccgagctcct ccacgcgcgg cccggctggc ggaccgaccg ctcggtccgg acgctcgcct
    13681 ggcgcttctc ccggcccggt ttcgcctatc gaacgttcca gctcctcgac tggcgcggga
    13741 cttctcgcgg ctacgccgtc gccgcgatcc gcgaggggcg cgtcgccctc gtcgacctcc
    13801 aggtcgcctc cgagtccgac ggcacgctcg tcgacctgct cgcggcgatc caggaggggc
    13861 tcggggcggt cgcggcggac cggatggtcc ttcgctcccc ccagcgaggg ctcctcgcct
    13921 cgcgtctcga gggagagctc gcgttcgtgc ccgagctctc ggacaccgtc ctcctgatgc
    13981 acccgatcgc cgacggcttc gacttcctcg ctccgggctc gtcgttcgac tatcgcttcg
    14041 ccgaccacga cgtcttctga gcgccggctg cgccgccgtc tacgcctggt tgcggcaggc
    14101 gtggcagatc ggcagctcct cgaagcgccc ttcgcggtgg acctgccgca gccgcgccag
    14161 cgccggcccc tcccagatcc cgcggacggt gtcccccttc gacaggtcgc cgacgatcag
    14221 ctcgtcctcg ctgtcgtggc agcacgccga gaccttgccg tcggcgtgga cggagaggtt
    14281 catccagagc atccggcacg gcgggcggcc cgcggccgcg gcggccggct gcggacgtgg
    14341 cgcgtcgctc cggtcctcga ccttccccgc ccagtcgttg aactcgttga tggcgatctc
    14401 gtcggccggc ccgaggagcg gccgccatcg ctcgaagaag gcggagatct gggccgcggt
    14461 ctccttcatc cgaatgatcc ggaggacgag gcgcggacgc ggcgccgggc cgcgcgcggc
    14521 caggaatcgc cggacgttcg cctcgacgac gccgaggtcc cctccgaccc ggacggccgc
    14581 gtaggtctcc ggctcgacgc cgtcgatcga gacgaccagg gcgtcgaggt cgaggtcgag
    14641 cagccgcgcc acgttccgct cgtccaggag cgtcccgttc gtcaggacga cgatcggccc
    14701 gatcctcttc gccttcgcgt gggcgaggag ctccgcccac ttcgggtgga gcatcggctc
    14761 gccgaagccc tgcgggaaga ggacgaagcc ggtctccgag gcgacctcgt cgacggcctt
    14821 ccggaagagc gcctcgtcca tcaggcgccg cttccggctc gtcgtcggca cgggacacat
    14881 gacgcaggcg aggttgcaga cggccgccag ctcgagcgcg aactcgaccg ggaaggcgat
    14941 cccctcccag aggtccggat cgtcggaggc gagccgcgcc cgccgctccg cgtgggtctc
    15001 cgccgcctcg tcccggaaga gggcgcggac ctcgagctcc gcgccctgcg ggacggcatc
    15061 gctcaccgcg cggccccgga ggagaggacg ggcagcgccg gggtcgcgtc ctcgtagccc
    15121 atcttcccaa gctggtggat gtcgtagcag tcgcagaagc gcggcgtcgt gcagcggaag
    15181 ggggcgttcc gccgctccag gcggcgctcc ttcaggttcc cgagatccgc gaggccgggg
    15241 atccaggggc agccctgcac ccgtccatcg ttccagatcc ggaagtagtc gcgcccggcc
    15301 gggcagagga ccgcctccct cccggcgaac ggggccgaga agtcgtcctt gtgctccttc
    15361 tcgacgacgg ccgcgtagcg ctcgaggagg ctctcgaaga gctcctcctg cgccgcgtcg
    15421 aatggccggt cgcagtcccg gatcgcccag accttccggc cggtcgccgc gaagaccgtg
    15481 tcgcggtagt acgcgagccc ttcctcccag aggtccgaga ggagcggcga gaagatcgtc
    15541 ccgaggatca gctcgccccg gtgcttctcg aggatcgttc gcgcgttcgt ctcccacgcc
    15601 ttctcgaccc tcttgtcccg cacctgcttg tagtggagcg tcaggttgac cgcgcggacc
    15661 ttcgcgagct tctcctccgc gagctccggg tactcgttca gcaggacgcc gttcacgtcg
    15721 agcgccacct cgacgtcgtg gtcgaggaga cggtcgagga agcgttcccg cttcgggtag
    15781 ccggcgaaga agtccccttt gccggagaag atgaagaacg tccgcgtccc gacctcgtcg
    15841 aggaagaagc gcacgtcctc gtagacgagg gacggctcgc gcttcacgac ggggtggatg
    15901 atgcagtagt cgcagtcgag ctggcagtgg cccgccgagg agcagaagac caccgtctcg
    15961 caggtcgtct tcaggtcggc cacgggcgac tcccgcgggg ccgatgggtg cgtctccgtc
    16021 tcattccttc gggatggtat cccagcgccc ggggtcggct tccgcccgcc cgagacccgc
    16081 cggcgcgcca gggcgcgtcc gccggtcccc gcgcttcggc gtcatccggc tccgccgcgg
    16141 acgaaggacg ccagcgcccg cgctccccga cgaagacccg acccggcgcc cgccggtcgt
    16201 gccccgaacg ccgccaggag cgcctcgccc gtccggccgg cgtgatacgt ccggccaatt
    16261 cgccgaccct cggtcccgag ccgcgcgcgg aggcccgggt cgtccgcgag gcggcggacc
    16321 gccgcctgga gcgcccccga atcgaagacc gggatccgga tcgaggcccg cgcgtcgggg
    16381 tcgatgccgc ggaaggcccc gatgtcggtc aggaccgtgg ggaggccgca cgacatcgcc
    16441 tcgatcgtcg ggaggccgaa gccctcgacc tccgtggacg gaccgacgaa gaggtcgacg
    16501 ttccgataga cgtcggggac gaaggcggga ggcacccaga tgtgacgctc ggcttccggc
    16561 caggccgcca cctcctcggg aggggcgtcc tgcgacatcc tcacgagccg cagccgcggg
    16621 atctcgccct tcagcgcgcg gagcgcttcc gtcgcccagg gaattccctt gaacgggacg
    16681 ctccactgcc ccgtcaggag gacccggaac gtcccgtcct gcgggcgcgc cccgctccgc
    16741 ggcgagaacg cgtcggcgtc gaacggctgg gggatgtgga ccgcctcctg cccgtagagc
    16801 tccttcacga gctcggcgag gtgcggcgag acgacgagct tcgtcgccgg gaggcggtag
    16861 acctcctcga tctcgggcca gaggggacgc gccggctcgt agatcgcctc gtacgcctgg
    16921 cagaagtgga acgctcgcgc ccccggtacg gccatcgcct gcgggacggt gtggtacatc
    16981 gtgccgaccg cgacgtcggc cggcccgatg gcctccggcg tcaggtcggg gacctgccgg
    17041 tagaacgacc acgactccgg cgcccagtcg ctcggcggcg tcgtgctgac gacctcggcg
    17101 tcgaccccga ggcggcgcag gatcgcgacg tgctggtaga cgaccttcac cccgccgcag
    17161 agcgcggtgc cgttgaggag gtagacgacc ttcacccgag cgtctcccag ccgaccggga
    17221 ccgtcatgaa gccgctcgtc cagcggtccg actccacccg gaaccggacg tcgctccggg
    17281 cgtcgtagag ggcgaggccc ttctcgtcgc cgaggaagat cacgaggatg aactccccct
    17341 tcgcgagcgg gagggactcg accgtcaggc ggatctcgtg ccgcgccttc ccgacgaagg
    17401 gccccgtgcc cgagaccttc gagtcggcga cgaagcaggt gacctggtcc ggcgtgacga
    17461 tcgcgacgtg gacgtggagg gggcgctccg gcgtgtcccc ctcgaacgcg agctccagga
    17521 cccaggggtc ccccggcgcg aagaccgccg actccctccc ggccccgtcg aggagccgga
    17581 tcgacgtgaa ccggccgagc gagtcctgga gggccgcggg cgccgccgcc gtgcgctcct
    17641 gctccttccg cgcgaggtac tcctcgtact ggagaacgac cttctgggcc tcgccgagcg
    17701 ccgcgacctc cccgtcccga agccagatcg ccgtctcgca gagcgtggag acgtagtaca
    17761 gggcgtggct gcagaggaag atcgtccggc cccgcgagcg gaggtcgtgg aggcggtcga
    17821 cgcacttctt ctggaagcgg ccgtccccga cggccagggc ctcgtcgagg atcacgacgt
    17881 cggggaggac cgccgtcgcg acggcgaacg ccaggcgcat cgtcatcccc gaggagtagg
    17941 tcctcaccgg ccggtcgaag aagtccccga gctccgcgaa ggcctcgatc tcggggagcg
    18001 ccgccgcgat ctcggccttc gtgaggccgg cgagcgcggc ctggaggacc gcgttccggc
    18061 ggccggtcac ctcggggtgg aacgaggagc cgagctcgag gatcgcggcg acgcgcccgg
    18121 ggaggtggat cgtccccgag gtcggctccg tcgtccccgc gaggatcttc aggagcgtcg
    18181 acttcccgga gccgttctcg ccgatgaccc cgaggaccga gccgcgggga accgtcgcgg
    18241 agacgccccg gagggcgtgg accggggtgt gccgggggcg ccccgtgagc gcctcgagga
    18301 ggcggtcgcg aggagacgca tagaggcgat agaccttcga aaggccctcg aaggcgaagg
    18361 cggcgtcact caccttcggt ccgggcggcg atgcacgagc ggcagatgaa gtcgtccggg
    18421 gcctcctcga cgccgtacga ccaccgccgc aggagggcga gggccggccc ctccaggacc
    18481 tcgcggacgc tctgcgtctt caggtcgccc acgacgagct tctcgtcgta gtcctcgcag
    18541 cagaagatgc acttcccctc cgccgtgacg tggaggtgct cgagcggccg ggagccgacg
    18601 ttgtcgcatc cccggagctt ccggtgcttc tccgcgggcc ggaccccggt cgggagccag
    18661 ccggcacggt cccgggcctc gaacggccgg acgttgaaac ggctcccgcc gtaccgctcg
    18721 gtgatctccc ggtgggcttt ctggtgcggc tcgtcccccc cgccgaggac gacgaggtcc
    18781 atctcctctg cgaccggctt gtcgcgcatg tagtcgaggt tccggacaac ggtctcgagc
    18841 tggtcgacgc cccgatcccg ggcgtagcgg tccttctcga gcgtcgagat gttcaccgag
    18901 aggtagcgca ggccgccgag gccgaggagc gcgtcgaccc ggtccggcgt caggccggag
    18961 ccgttcgaga ggacggccgg cggaaggccc gccgccttga tcgtcgccac ctgctggacg
    19021 aaccggcggt cgaccgtcgg ctcgttgtag ttgttcatgc agacgccctc gatcgtgtcg
    19081 cggaaggcgg aaagctccgc gacgatccgc tcgtagagct cggtcggcat gaagctcttc
    19141 tcgcgggggg cgaccgagac cgggcagaag tagcacgcct ggttgcagac ggagtgggcc
    19201 tcgagcgaca cgaccttgag gcggaaccgc gtcgagaggt cggtgccgtc gggaatcgcc
    19261 cagagcccgt cctgcagctc ccgcccgatc gcgccgtcga cgggacgaga ggcccggagg
    19321 tcgtggagcg gggcgaagag gcggtccgcc ggggtcagcg tgacattcgt gagggggttg
    19381 acgacctgcc agccgtcgaa atggaggtag gggctcgcca ccaggcgagc cgcgccggag
    19441 aggggcgcgg cggatcggga gacgtcgtcc ggggaggcgt tcattcggac ggattatcca
    19501 cgcgcccccg gcgccgtccg gcaacgtccc ggccgcgccg cgccgcgtcg ttgcggtacc
    19561 ctcccgccgt gccatcccgg cccgcccccg ccctgccgcg ccccgcccgc ggggcgctcc
    19621 tcgccgtcgc cgcggcgctc ctccttacgg ccgacgaccg gcacgcgggg aaggtctccg
    19681 acgggcggca gacgatcggc acggccgtcg cgatcgtcga gacgggcgag ctcgggatcg
    19741 ggaagggggc cccgcgcgcg ttcgagcgcg aggccgacgc cgtctcgcgc tacggcctcg
    19801 gggcgagtct cgcccagctc ccggcggcgg ccgccgcgcc cgccgtcgag ggcgcgctcg
    19861 ggcccggcac ctcccagccc ctcttcctcc tcgcgccgct cgcgggggtc ctcctcgcgg
    19921 cggcggccgc aggcgggatc gcccggctcg ccggggcctc gacctcgctc caggggctcg
    19981 cgatcctcct cgcgtcgctc ggcgggccgc tcgggtcgta tgccacctcc gacttctccg
    20041 aggctctcca ggcggccgcc ctggccggcg cgctgctcct cgccctcggc gccgtccgag
    20101 aggacgaccc gcgcgtctcc cgccgccgcg cgttcggcgc cggcctcgct gccggagccg
    20161 cgctcctcgt caagtcgagc ctcgccgccg ttgcgccgat cctcctcctc ccgctcttcg
    20221 ccgcgtccgc gctccgcgga cggcggctcg cggccgccgc ggccggcgcc gctctccccg
    20281 tcgccgcatg ggtcgccttc gagctccacc gcttcggccg gctcttctcg ggctacgaag
    20341 gggagggctt cacgcacccg ctccccgagg gcctctggcg gctcctcgtc gggccgaatc
    20401 gcggcctcgt cctcttcttc ccggcggcgc tcctcgcggc cgcagctctc tcgcgcgcgc
    20461 tccgccgccc gggcgacgcg ccgctctgtc tcgccgcggc cggctcggtc ctcgcgttcc
    20521 tggtcctcct cgccaccgcc gccgcctggt gggcctggca cggcgtcggc ggttggggac
    20581 cgcgtctcct catccccgcc gtgccgctcc ttgcgccgtg ggccgcgctc gccgccgagg
    20641 gcctcggcac cgggggccga cgggccgtcg ccgtcctctc ggtggcgctc aaccttccgc
    20701 cgctcctcgt ccacccctcg ctcgtcgaca cctacgtcgc gaactgccgg cgcgccccgc
    20761 tcacgccgga gctcgagcgc gtggtccccc ctctcgccgt cggccccgac gacgcgggga
    20821 ggccgagcgt ctcccccgag aacgtcctct cgaaggagcc gctcgcctcg ccgttcctcc
    20881 tctacccgtg gtacgcggcc gcctccgcgc cgcgctccgc ggagagccgg gcggagcggc
    20941 tcgcccgtcc gccctggtac gagcgccggc ccgagctcgg gccgcgcctc gtgccgttcc
    21001 cgccggagct cgccgacgtc gtcgcgccgc cgctgggctg gagcttcttc ggtcgcgcgc
    21061 tcgggcgggg gatcgcggac ccggcgtcct tcgccgtcta cctcgcctcc ctctccgagc
    21121 aggtcgggcg ggcgcacgag gcggggcggc tcgagcgggc cctcgagctc gctcgaaagc
    21181 tcgcgcgcct cgacccgggc gaagggtccg acgccctcct cctcgagagc ctgcgcctcc
    21241 tccgccgaga ggagaccctc cgcgcggcgc tggcggcgct ccccgaggag cgggccgccg
    21301 cccccgccgt cctcgtcgtg cgcgcgctcg ccgcccgcga cgccggggac ctcgccctcg
    21361 ccgcgcgcct cggcgaggcg gccgccccct tcttccccgg gaccgccctc gggcgcgacg
    21421 gagcccgccc cgcgtcctgg ccggcgacgt tcgccgcgct cacgcgcccg ctcgttccgt
    21481 ccgcgtgagg cctccgtctc cctcccgctg ccgtagactt ccccgcctca cggaggcctc
    21541 atgaccacga gccagcacaa gaacatggcc atcctcgtcg gcggcgggcc ggcgcccggc
    21601 atcaacagcg tcatcagcgc ggccacgatc cgcgccgagc tcgagggcgt ctccgtcctc
    21661 ggcatccgcg acggcttcca gtggctcatg caggggaaca tcgagcacgt ccacccgctc
    21721 acgatcgaag aggtgagccg gatccacttc cgcggcggct cgcacatcgg catcgcccgc
    21781 gcgaacccga cgaaggaccc gcagctcctc gagaacgccg tcatctcgct ccttcgcctc
    21841 aacgtctccc gcctcgtcac gatcgggggc gacgacacgg ccttctccgc gatgaagctc
    21901 gccgagaagg ccgacggccg catcgccgtc gtccacgtcc cgaagacgat cgacaacgac
    21961 ctcgacctcc cgcccgacgt cgacaccttc ggcttccaga cggcgcgcca nnnnnnnnnn
    22021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gacggcgtgg cgatcatcgc cgaggggctc
    22141 gtcctcgagc tcgaccccga ggagctgaag gggctcgcgg aggccgagag ggacgcccac
    22201 gggcacctcc ggatcgccga cgtgaacatc ggcgagatcc tgaagatggc cgtccaggag
    22261 gagctgaaga agctcggcgt gaaggcgacc gtcacggaca agaacatcgg ctacgagctc
    22321 cgctgcgccg acccgatccc gttcgacctc gagtacacgc gcgacctcgg gtactgcgcg
    22381 gccaagtaca tcctctccgg cggcacgggg gcgatggtct cgatgcaggg ggggaacttc
    22441 gtcgcgatcc cgttcgagca gatgctcgac ccggcgacgg gacggacgaa agtgaggatg
    22501 gtggacgtcc actcgacccg gtacgcgatc gcgcgccgct acatgatccg gctccggaag
    22561 gacgacttcg aggacccgca cgagctcgcg aagttcgcgg ccacctgcgg catgtcgctc
    22621 gccgacttcc ggcagcgctt cggctacctc gtcgagaacg agctcccgcc gatcgttctc
    22681 ctgccctcgg gtcaccgcga cgagaccggc cccgggagcg ggtcgtactt cgagggggag
    22741 acgctgtagc cggcgggttc ggggcggccg gggaccgcct gggcgatact gggaagtcgc
    22801 ggagggagcg ggcgtccgtt ccctcttccg gggagacccg gcagaggtcg ttcatggccg
    22861 agatccggtg gtgcacgggg gagacgggcc gttccgagcc cttgggcggc gacggcccgc
    22921 tcggcctggg ccgcgagccc gacaaccact tcgtcctcgc cgacccggga gtctcccgcc
    22981 accacgcgcg cctcgtgcgg agcggggacg attgggcgat cgaggacctc ggcagctcca
    23041 acggcacctg cgtgaacggg cgccgcgtct ccagagctct cctgaaagac ggcgaccgcg
    23101 tccggctctg cggagtcgag ctcgagtttc gcgacgtccc ggcggccggc acgccgcccg
    23161 acgccgccga cggggacgcg agctacttcc tcgcccgggg cggccaacgc ctcggcccgt
    23221 acgcctggga ggacctgacc ggcttcgcgg cgaacggaca ggttgcgcct gacgacgagc
    23281 tctggggccc cggcctcgat cgatggaccc cggcgactgc gatccccggc ctccttcccc
    23341 cggtccccgc gcctctcccc cgccccaccc cgaagcggcc cgaaacctcc gcgcggcgac
    23401 gctcgccgtt cgcacggggt cttctcgtcg cggcgccagc ggccgcgctc ctcctcctgc
    23461 tcggggccct cggctggtgg ttctgggggt cgcacccggg cgcggacggc ggcgacgcag
    23521 gcgacgggaa tcgacgggcc atcgagaccg cgaccgcgca ggcgcccccg gcgccccccc
    23581 cgcccgtcga cgactggaag gagtacccgg acctcgaagc cgagcggcgg cggatcggcg
    23641 acagcctcag cgcgttccag cgggcgctga gggccggcgc ggtggacgag gcggtctcct
    23701 ggatcgccac ggagcggcag gcgacgtacg ccgccctgtt ccgcaacaag ccggcggcga
    23761 tggcctcctt cgccgacctg ctcgagagat cgcagctcac cttcctcgcg ccccccgcgg
    23821 agccgtccgt cgcgacgacg gtccggaccg ccgagtacac ggtgacgatc gacggattcg
    23881 acttctacct ccgatggtgc cggagggacg accgatgggt cctcttcgac ttctgagggg
    23941 cgcccggacg gcgcgcgtcg cctccggcgc gctcgcgctg acgattctgg gcgtcgtgac
    24001 gccgcttcgg ggctggacgc aggcccccca caggcagatc aatgccgagg ccgtggccgc
    24061 gttcttcgcg aagatggaac gggagccgtc cgagaagttc cggctcgggc cggtcccgac
    24121 cgacgagcgc aaggccctgc accgcggcat cgccgtgacg agctcgacga tgttcgtcaa
    24181 gccgtcggga ggcctcgcag aggcctacga gatcggcgaa gcgcgtcacg ccatgcccgc
    24241 ctggatcagc ctcggcggcg actgggccga cgagccgcac ctctacgcct cggtgaggca
    24301 cttctacgac ccgctccagg tggcgggggt gccgttcctg acggatcagc tctgggcgca
    24361 cggcacctac gacagcccgg ggatcgaggc cgtgacctgg gccctcgacc acccggacaa
    24421 tcccttcagc cttcgtcaag gaatggtcgc gtacaagctc gcgatggaga ctccggagga
    24481 cgtcccgcct cctcccggtc tccgagagag ccacttcaag acgaacgtaa gcctcgtgcc
    24541 ggggaacgcg gccgagcagc gttccctcta cctcgcccgc gcctaccggg cgctcggcga
    24601 ggcgatgcac atgatcggcg acatgaccca gccggcccac gtgcgaaacg actcgcaccc
    24661 gatggacgag ccgatcgagt ccgccgtgac gacctcggac gtccgcctcg ccgccgcgag
    24721 ccccgtcatc gacgcggcct tgaggcccta cctctcaagt gccggcggga cgctccaggc
    24781 gccgagggcg ctttcccgcc aggtcgctct cttcacgaat cgcaccttct actcgatgga
    24841 cacgatcttc gaccggcgca ccgggacgct cccgacgaac ggcatgccgc cctatccctc
    24901 gccccagttc ggcgacctcg tggtccggac ggccacggtc cggggcttcg tcgggaagcg
    24961 ggccgtgacg aagctgctcg gcagggtcgc ggggcgcgag gtcccgatgg cccaggaacg
    25021 cctcagcttc cactggttcg accccgatca ctcggtgatc ttcggccccg ggctcgagac
    25081 cgcctcgggc ttcacgagca agctcggtcc gtacatggtt ccccaggcgt tcgcccgcga
    25141 gcaggcggcc gccctcctgc ccgtcgccat ccacgcctgc gccgacctga tggacctctt
    25201 cttccccacc ctcgagctga aggcggagta ccggctcgag gggcccggcc cggacggctc
    25261 ctcggggcgt caggtcgtcc gtatcgagcc cgggatggtc cacctccgcg agcgcgaccc
    25321 cgcatggtcg gccttcgacc tcgacgtcgc gtacaccggg cccggcgccc tcgtcatcac
    25381 ccgcggcggg aaggtggagg ccacccggaa gctgcacttc cggaagggcc tcctcgacac
    25441 gatcgagaac gtcgcgggaa agatggtgaa ggctccgctc cgagtcttcg tgtcggacgg
    25501 tcgaaccgct ctgaccggcg aggaggagct ctactccttc gcgcacggcc agaagctcca
    25561 cgtcgagatc gactccggca gccgccattt cgagagcccg ccgtacgagc tccggcacga
    25621 ggtcgtcatc cagcctcggc gcggcgtcgg accgcccgga gccacgttcg acttcgaagc
    25681 tcacgcccgg cccgagggcg tctaccgctt cgaatggtcg tggccgggcg cgggcgaggt
    25741 cgtggcgacc gagggctcga cgtccaaagt ggccccggtc ctcgtccggg aaggcgagta
    25801 cccgttcacc gtgaagctcg tccatcccga cgggaccgtg ctggccgagg accgcgccac
    25861 cgccgcggtc gagaaggaga cggccgcccc tccgacgccg gtggcgaaga agggccccgg
    25921 gcgatgggtg ctcgtgaggc aatggaacga cgtctggaag gacccacggg tcacgagctc
    25981 ccgggcgacg gcggaggccg gcgtcaactc cgcgaagatt cacgtgacgg tgcccgaccg
    26041 gcagacgacg tggcaattcg actacgcaat gagctggagc cctcttccgg cctcggtcgg
    26101 acgggacgag accttcgacg tgacgcttcg actcgactcg atccgggtcc ccgagccacg
    26161 ggtaggctgg ggcgctgaga tcgccgtgtc cacgggccgg acagaggcga tacgcgcgac
    26221 ggcgcctccc aaggccgcga acttcgtcag tcagacgaca tggaacggga gcgtgctcca
    26281 gggagggttg gggtcagccg cccggaagcc gcccgacagt cggtccgtta ccctcgacct
    26341 cggctggaga agccccgagg acggggacgt ccaggtcgtc acggtgcgaa cgtgggggca
    26401 gggagccggg gccggcggga acggcgccgc gatccacttc cactacgagt accgcttcca
    26461 ggaataggaa ggtcgccgaa gccccgtcct gccgcacgcc tcgaccggca ggagactccc
    26521 ttttcgcccg gccaccgcga cgagaccggc ctcgggagcg ggtcgttctt cgagggggag
    26581 acgccctggc cggtcacgcc ttcgccgaag gccccggctg tccgagcagc cgaacgacgg
    26641 gctcgatcga gcgccaccac ggttccctgg cgcgctcgaa ctccggctcg accatcgacg
    26701 cgagagcgtc cagcgggaac gccgtcaggc ggcccgactg gacgccgagg aagacggccg
    26761 cggggctctc cttcgcgctc tcctcgagga ggacctccac cgcccgggcc gcgtagcggg
    26821 tcgccacgat cctgtcgaag ggggtcgggt cgcccccctg ctggaggtgc ccgaggatcg
    26881 cctgccgcgc gtcgaacgcg ccccggccct ccgcctcgaa cagccgccgg aggaagtccg
    26941 tcgtgaagac ggggctcgcg ttctcgttcc ggatcatcag cgcgaggcgc ttccccttcc
    27001 ggaacccctc gacgaggctc gccacgtcgc gctcgagcgc cttcatcgtg atcccttcct
    27061 cgtgggtgaa gacccgctcg gctccggtcg cgaggccgga gaggaccgcg aggtagccgc
    27121 accagcgccc catcacctcc acgacgaacg tccgcttcca ggcgacggcc gactgcttga
    27181 tccggtccac cgcctcgacg atcgcgttca gcgccgtgtc ggcgccgatc gcgagctcgg
    27241 agccggggag ggagttgtcg atccccgccg gcacgcagac gagcgggacg cggaaggccg
    27301 cgtgctccgc gcgcgccgac tgcatccggc ccgccgcctg gtaggccgcc cagccgccga
    27361 cgaggagtac gccttcgacc tggcgcgccg ccagcacctc ggcgagcgcc ccgaggtcct
    27421 ccgccgcagg gaccctgcgg ctcgtcccga gctcggcgcc gccgagcgag acccagccgt
    27481 tcacgctcat ccagtcgagc ggcacgacgt cgccggcgag gagcccgtcg aatccgcccc
    27541 gcgcgccgag gacgacgtgc ccctggtcga cgaggaggcg gaccgccgcg cgcacggcgg
    27601 tgttcatccc cggcgcgggc ggtccggcgt ggaggacgag gaaccgcctc cgccgctgac
    27661 cgtctcgcgg cccgtgggga aaggcacgga cgagcgtctt catgatccgg aaggagctcc
    27721 ggaaggacgt cccgcggagc gtcatcgccc gctcgaagtc gcctgagcgg atcgcctcgc
    27781 cgactgcccg cgccttctcg acgttctcca ggagcggcac gcgcgtcacg cggttctcgc
    27841 ggagcccgat gaggcagggg acgtcgtcgg gcccccagcg gacgacctcc tcgatcgccg
    27901 cgacgccgag gagcgtcccc atcgtccggt cgaaggcgct cggggcgccg ccgcgctgga
    27961 catggccgag gacggtcgtc cgcgtctccg tcccgaggcg ctcctcgagg agcttccgga
    28021 tccgctccgc ctcgatgggg cgcccctgcc ggtcgaccgc cccttcggcc aggatcaccg
    28081 tcgagtggcg gcggcccgcc tttcgcccct cggccaggac ggcgcagagc ttctcttccc
    28141 agtcgtcccc ctcgggcggc gcctcgggga tgaagaccca cccggcgccc gtcgcgagcg
    28201 cgctcatcag cgccaggtag ccgcagtggc gccccatcac ctccacgacg aaggtcctct
    28261 ggtggctggc ggcggtggag acgagcgcgt ccacggcttc cacgatccgg tgcagggccg
    28321 tgtcggcgcc gatcgtcatg tcggtcccgg ccatgtcgtt gtcaatcgag ccgacgaggc
    28381 cgacgagcgc gagaggcgac gcgctcctcg ccgcctcggg cgggatctcc ccgccggcgg
    28441 cgagctcctt caggagagag ggccactcgc gccggaagag gtccgcgccg gtcagcgacc
    28501 cgtccccgcc gacgacgatc agggcctcga ccctctcgcg ggccaggttc cgggcggcgg
    28561 cgagacggcc ttcgcgggtg cggaacgccg gacagcgcgc cgtgccgatg acggtccccc
    28621 ccttggggag gatgccgccg acatcgcccc aggccatccg gcggaatcgc tctcccccgt
    28681 cgacgagccc ctggaagcct tcctccacgg cgaggacctc gagccccttc tcgagcgccg
    28741 cgcggaccgc ggcccggacg acggcgttca ttccgggggc gtctcccccg ctcgtcagga
    28801 tcgccacacg gcgcgggctc tcgctcggca tcctcgtctc ctccgggccg gcagcgccgg
    28861 cctgcgaccg gacatcctaa cggagcgtgc cgggcgacgt cgccgcgtcg cgggcgtccg
    28921 ggaggccggg cagctcccgc ttcggcgcgc cgagcgcgaa cccctgcccg tactcgacgc
    28981 ccagctcgcg aaggatggcc accgcgtcga ggctctcgac gtgctcggcg atcgtctcct
    29041 tgccgagcgt gtgggccacg gtgtcgatcg ccttcacgag cgcgcggttc gacgggttcg
    29101 tctcgaggtc gcggatgaac gagccgtcga tcttgacgta gtccgccggc agcccctgca
    29161 ggtaggagaa cgaagagaag ccgatcccga agtcgtcgag cgcgaagcgg cagccgaggt
    29221 ctcgcaggcg ccgcatccac tcccgggcgc cgataagatc ccggacggcc gtcgtctcgg
    29281 tcagctcgaa cgcgaggcgc cccgggccga tgccgctctc ccggatccgt tcctcgaggg
    29341 tggcgaggag cccctcctca ccgaggctcg cgccggagag gttcacgaag acctccacgt
    29401 cctcccgggt ccggaggacc gaaagaacct cgtcgacgac ccacgcgtcg atctgcggca
    29461 ggagcccgaa ctgctccgcc gcgggaagga agtcccccgg cgccacgagc gtcccgtcgc
    29521 cgtccgaaag ccggaggagc gcctcgtagt gcgccgtccg gccgctctcg agccgcagga
    29581 tcggctggta gacgaggacg aaacgcccct cgcggagcgc gtccttcacg cgcgaggccc
    29641 agaggctcgc ctcagagagc gtcgtcggcg ccacggagcc gtcgaagagg acgacccggt
    29701 tccgtcccct ctccttcgcg gcgtagagcg ccgagtcggc gagtgcgagg agcgcgggcg
    29761 gcttcaggcg gccgtcgatc gggacgaccc cgacgctcac cccgaggtcg aaccttcttc
    29821 cttccacctc gaaccggtgc tcgtgcacgg cccggcggag gcgctcggcc accatccgtc
    29881 ccgcctcggc gggaatcccc tcgaggacca ggacgaactc gtcgcccccg agccgggcca
    29941 tctcgtcgcc cggacgccgg agcgtcccga ggaggcgcgc gagctcgacg aggacggcgt
    30001 cccccgcccc gtgccccagc gtgtcgttga cgaccttgaa gtggtcgagg tcgaggagga
    30061 ggagagagcc gggcgtgccg cggcgcgccc gggccacgat ccgctcgagg ctctcctccg
    30121 cggcgcgccg gttcccgagg tccgtgagag ggtcgtgcag ggcgaggtag cggatccgct
    30181 cctccgtgcg gcgccgctgc gtcacgtcct gcttcacggc gacgaagtgg cggatccccc
    30241 cgtcggcgcc ccggaccggg gtgatcgtct gctcctcgac gtagacggtc ccgtccttcc
    30301 ggcggttgta gagctcgccg cgccagacgt ttccctcgag gatcgtctcc cacatccgtt
    30361 cgtagaagaa ctcgctctgg atccccgact tcagcacccg cggcgtctcg ccccggagct
    30421 cctccgcgga gaatccggtc agctccgtga aggcgcggtt cacccactcg acgcgccctt
    30481 cgcggtccgt gatgacgacg gcgttcgcgg tcgcctccag ggcggccgtc tggagctcga
    30541 tccgctcgag ctgctcggcc tccaggagcg agagtgcgac ctggtccgcg acccgggcga
    30601 tcgggacgac ggcggcgccg gcgaacgcct ccggccccct cgcgtagcag gagaggacgc
    30661 cgaggaccct gtcgccgacg acgagcggca cggaaagcgc cgcgccgagg cccgtcgcgg
    30721 ccgcggcgtc ccgccaccgc gccacccggt cgtcccccag gccgtcgacg aagaccggcc
    30781 ggccggtccg gaccgcctcg ccggacggcc cgcgtccctc cggcgacccg tcccaacgga
    30841 acgtcgatcg ggccacgaag cccgaggcgc tcccggcgac cgcgcgcggc tcgatccttc
    30901 cgtcgggctg cttcagcccg atccacaccg ccgggaggtc gaagagctcc gagacgcgcg
    30961 tgcagatccg ggcgaggatg accgtcggct cctcgcggcg gaggattccg cgttccgtct
    31021 cgtgctgcag gcgcaggacc tcctcgtccc gcttccgccc ggtgacgtcg cggctcgtga
    31081 cgacgagccc gtcggactcg ccgcgctctc ccttcgcgag cgtgacgatc gagtcgaccc
    31141 agagccagcg cccgtccttc gtccggaacc tgtaggtgaa cgccgacgcg ccgtcgcgac
    31201 gcgccctccg cccgagcatc tcccgcgaga gctccgcgtc ctccgggtgg acgaactcca
    31261 gcgcgttgtg cccgaggaac tcctcggggg accagcccgt cgccgcgacg tacgaaggcg
    31321 tcgcgaagcg gaggttcccg tgcaggtccg tctcggtgat gacgtcgatc atgttctcgg
    31381 tcaggcgctt gagctcgcgc tcccgctcgc ggagctcccg ctcgcgctcg acccggtccg
    31441 tcacgtcgcg gacgatggag aggaccgaat ccgcgtcgtg agggacgacg cgcccctcga
    31501 aggcccggcg tccggcctgg aggtcgagct cgtactcgat cacctgcatc ccgccccctc
    31561 cgaggcagcg tcggatggcg tccatccaga cgtcggccgc ctccttcggc aggacctcgg
    31621 gcacggagga gcccaggagg ctctcccggg gggcggcgag cgcctcgggg cgggccgcgt
    31681 ggaccgcgag gtagcgcccc tcgcggtcga ggacgaagag gaggtcgggg agcgcctcga
    31741 ggacggcccg gtggcgcatc tcgctcagcc gaagcgcctc ctccgcccgc cgcctctcga
    31801 tggcgagcgc gacctgggac gagacgaagc cgaggagggc ctggtcctcc tccccgtagc
    31861 gcacgttccc cgcgtagctc tggacggcga ggacgccgaa ggtcctctcc ccggcccgga
    31921 gaggaacacc gagccagtcg atcgaggccg cgccgatcgg cgccacctcg ccccgcgcca
    31981 cgagggcgtc gaacacctcg ggcgaggcga ggaggggctc tccggtccgg aggacgtagc
    32041 ccgtcaggcc ccgccccgcc gggaccggcc cctccggcgg ctccgcgtcg atctcgtccc
    32101 ggaagtaggg gaaggagagg ctcccggtcg cctcgtcgag aagcgcgatg tagaggttcc
    32161 tcgcctccat cagcccgccg acgatcctgt ggatccgggc gaacaggtcg tccaggccgg
    32221 cggtcgtcgt caccgcgtga gcgatggcgt aggtggcctc ctggatccgc ctctggcggc
    32281 ggtaccgctc cgatcccccg ccgtccgcgg gctcgtccgt cctcatgtgc gcaccgaagg
    32341 gtggatgtta gccggtggcc cccgctttcg ctcccgggag gagaatccga aggccccggg
    32401 gccggaagcc tgcgcgacgg cgtccaccgg cgcgtccggc accgcgagga gacggggctt
    32461 gacagccgga tcaaatataa ctatattttc atatagtcac acatcggggg cgttccgccc
    32521 ccgccggcaa ggaggagaca tgcgatacgg agtggcggcg gcggtcctgg ggctcctggc
    32581 ctcgggcgcc gtccgcgcgg acgaaccgct caccctcggg gaggcgatgg cccgcgcccg
    32641 cgacggcgcc cgcgaggtgg ccgccgcgac ggcccggaag gaggcggcct cggcccgcgc
    32701 tcggcaggcg tcggcgttcc ggctcccctc cctcaccctc tccgagacgt acgtccggac
    32761 gagctccccc gtcgactcgt tcggcctcga gctgaaccgg gaggagttca cgttccagaa
    32821 gttcgtggcg accgacccga acgacccggg ctggtccggg acggcgatca cgcgcctcga
    32881 ggcgcaggcg ccgatcttca ccggaggcga gctctcgggg cggatcgcgc aggcgcggag
    32941 cgcggccgac gccgccgcaa gccaggcctc ctgggcggcc gacaacgccg ccctcgccgc
    33001 cggcgaggcg tgggtgatgc tcgcgcaggc cgaggagttc gtcgccctcc tcacgaaggc
    33061 gcgcgacacc gtcgcggccc acgtcgcgct cgcgaggagc tacgtcgagc aggggatgct
    33121 cgtccgctcc gagctcctcc gggccgaggt ggagctcgcg cgcgtcgagg acctcctcga
    33181 ggacgcgaag gggaaggccc gggtcgcgaa cgcgaacctc gcgttccgga tcggcgtccc
    33241 ccaggactcc acctggaagc tcgcgcccct cccgggcccc gggccgctcg acggcgacgc
    33301 ctcgcactgg gtctcctccg ccgacgcccg caaggacctc tcggcggcgc ggagcctcct
    33361 ccgggccggc gagctcgagg aaaaggtgaa gaaggccgcc tggctcccga aggtgggcgt
    33421 cctggcccgc tgggacctcc acgacaagaa cctcttcggc gcgagcggct cggccggcac
    33481 cgtcctggcg ttcgcctcgc tgaacctctt ccaggggggc gccgacaagg ccgccctcgt
    33541 cgccgcccgc gaggaagccc gcgccggccg cgaggacgtc gcccgcttcg cggacggcgt
    33601 cgccctcgag gtgaggcagg cgtacgagga ggccgccacg gcccgcgccc gccacgcgac
    33661 ggccgtgaag gcgctcgagg ccgcccgcga gagcgagcgg atcaccgacg agcgcttccg
    33721 gagcggcgtc gtgaagacga tcgacctcct cgacgcggcg accgcccg
//
LOCUS       DAOTEZ010000003        32884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_887_length_32884_cov_6.927990, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000003 DAOTEZ010000000
VERSION     DAOTEZ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 32884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32884
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_887_length_32884_cov_6.927990"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="P5164_00260"
     CDS             <1..603
                     /locus_tag="P5164_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42348.1"
                     /translation="WLLVHQVLPFVPLAGAAWAALSASRKRALAVAAPAFAATLVSAL
                     SLSFWNPLLSPAHPRAESVVFTQTSPEVMPVVAEILRAGGSGEDPAAAVSGEAVWPLS
                     WYLRGLPVLWALPSAGTRPPVVVVDEGKVEEAQRLLGGGYAVETIPLRSWWLPEVSLS
                     PLRPNARELLVYLFTRRPWSPVGAQNVVVLRRPDGGRPSR"
     gene            600..1568
                     /locus_tag="P5164_00265"
     CDS             600..1568
                     /locus_tag="P5164_00265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY42349.1"
                     /translation="MSAPEISVVLPCFNEVENIPELRRRLVAVLEAAGRSFEVVFVDD
                     GSRDGSWEAMAAQAAEDPRLRAIRLSRNFGHQMALTAGVDGARGRLVAVMDADLQDPP
                     ELLAEMLSKADEGYEVVYAQRSAREGESAFKLATAHLFYRLMRRWTNVDIPVDTGDFR
                     LMGPKAVAAFRSLRERHRFIRGMTAWVGFRQVAVSYVRPPRLHGETKFPLRRMVRFAL
                     DALTSFSHVPLQLGTWLGFGVSLFAFLYILVVLVLKVLGINERGWTTLMGWMLLLGGV
                     QLMLIGVLGEYLGRIYDEVKRRPLYLVSEEAGGPERDTAPEADPAR"
     gene            1622..2527
                     /locus_tag="P5164_00270"
     CDS             1622..2527
                     /locus_tag="P5164_00270"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HRY42350.1"
                     /translation="MRRVLSLLVLGLIGLLSGPLAADQVVLRDGRTLDTKKPPVIKGR
                     QAVLTLADGKLVSIPAAEIDQEKTAALAKKAAEAPKAAPAETPRAPSLVDAARATQNA
                     RKAAVVLTDQDVAGGYLDVSSGSPEKGEGEVSIGPTTTKKSDAGYVIEGSVLNSGKAP
                     VLGVSVTVEAVGADGKSIATTYAALAKDVLDPGEKASFRAQIDTDKTVERFGYVPRWK
                     VIAPPAKEGEPGERAEQKKEPGAAGEEAPPPPPPPAKEEAPPQRPPDMAAPPANAPVG
                     APSQPGGSYLPPPSSNQPKPPGGRG"
     gene            2550..3911
                     /locus_tag="P5164_00275"
     CDS             2550..3911
                     /locus_tag="P5164_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRY42351.1"
                     /translation="MAAEILLVEDRESLRSMLAETLGGEGYRVEAVAAGDEAVRRLSE
                     GRRYALVLTDLKLPGADGLAVLESALANDPSVPVVLLTGYGTVETAVAAMKRGAADFL
                     NKPVDVDLLLLLVKRHVEARRSAVARALLAEEAGLAGMPRILGSSPALADALDRLRRA
                     APSDVTVLLTGESGTGKELFARALHALSPRRAGPFVAVNVAALPEGLVENELFGHEKG
                     AYTGAGERRAGRFELADGGTLFLDEIGELPLGAQTKLLRAVEERTFLRVGGTVPLSVD
                     VRLVVATNRDLAQRVKAGAFREDLYYRLDVFPVKLPPLRARREDVPELARAFARSCGE
                     GARGRAFEIGAGAAEKLAAWNWPGNVRELKNVVERAVLLAEGSVIRPADVVLGAPGED
                     APTTPTLDGTLEEAGERWKRAGEAARIRRALEEAGGDRAAAAAALDIPLKRLAQRLRD
                     LKL"
     gene            complement(3908..5287)
                     /gene="selA"
                     /locus_tag="P5164_00280"
     CDS             complement(3908..5287)
                     /gene="selA"
                     /locus_tag="P5164_00280"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HRY42352.1"
                     /translation="MARPAVLPPSIEALLSSERGRALAGRHGRLRVRELLREGAARLR
                     SGTLEPGADPAATLLDGAAAALAARPPAGPRPVLNATGVLLHTNLGRAPLATAAREAI
                     LRAAGYSTLELDLDTGGRGRRGEHVRPLLLSLFAPGRPGVDALAVTNAAAALLLALDT
                     VANGRPVAVSRGELVAIGGDFRVPSILAKSGASLLEVGTTNKTTPDDYVEALRVGAAA
                     VLKVRPSNYRVVGFTEEVALPELAGLCHEAGVPLLYDAGAGSPERFAEPGLCDEPVPA
                     EALAEGADLVCFSGDKTLGGPQAGILLGREDLVRSCARSPLARALRPDKLVLAALAAT
                     LDLHLSGRAAEVPFHAMLAAPLADLEARARRLAAVAAAAGWSAEAAPLVAIAGGGAGT
                     ESTLPSWGVLLRHPSLGEGDAAARLRARELPVLARVADGRLVLDLRSVSPDEDADLAS
                     ALREAGSEV"
     gene            5311..6012
                     /locus_tag="P5164_00285"
     CDS             5311..6012
                     /locus_tag="P5164_00285"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HRY42353.1"
                     /translation="MGISIERELVFLERNITALRVEYERFFGGELKRPPVQSRRTIED
                     VLRRAGNAEVEKAAERFRLQSLQSRYNSFVEIWDKRMRAKEEGTTGPVRRLASSTPAV
                     KPEAPPASTKPAAKKADSAADVAALLAAAEAAVARKQPPPAPAPDAPAAPSVKPKSRV
                     DFTPLFQRYVAARQALGEDVTKLRYEKFEEAVRRQAEEIRKRTGSSRLVFEVQTAEGR
                     VKLVGRPVPAPGSKG"
     gene            6015..7019
                     /locus_tag="P5164_00290"
     CDS             6015..7019
                     /locus_tag="P5164_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY42354.1"
                     /translation="MLAPFPKALLPGAIAVSLLLAPVAAGKDVYRPYLDKAIPHHAAI
                     LDTLEKLEKSPEDAQLHNDLGCLVAWDGFWRDALRSFDEASDLDPKDSRPWFNAGLVQ
                     ALRGEWGAARSRFRKAVKVDPGNWSAWWMLGFAEETLGHERAAVEAYSRSLRVDTSLF
                     DPRVNPFAVATRLKSRVLLRTYERRRVDAALPFSNQLSDASRLAAFFQASPAPAKSAA
                     PAPEPARTGPVVTAVPSSTVLPDAAASEPAQTRPSRRRRAYPRGSDPSEPVELQPEPP
                     RQEPEPAAAEPKPEGGDAPRTPGPGGGGPGLGSGARRVPGPGGAPPAEPQPEPQPEPR
                     "
     gene            complement(7042..8382)
                     /locus_tag="P5164_00295"
     CDS             complement(7042..8382)
                     /locus_tag="P5164_00295"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HRY42355.1"
                     /translation="MTPASDDGLFERVLEAGSTDGCGAELYERRGRLLELTEDETGTS
                     LLESGERGLALRLFKGGRSAFAASGLAHAFSLPDQARARLPRARTRRGVRLPAPAAGE
                     ALEPRPIGDPPAPNVDLARTLLTGFRQALTSSGRGSVAVREASVSLGERTERLATSSG
                     RDTAWGSRLATLVATVVGRSTGGRFSARVVGAAPRPEELPVARLARHAADRVLLPLQG
                     RPFEAARADVLLDPLVAAHLVGRLAPLFFGDDEEVLLAARTRDGRDALASPLLSVVDD
                     ATVSGGLVRGVRDGEGTPQGRTRLVERGLPSGRLTDSAAASRLSRPLSGNAIRVAWSD
                     APRIGVTNFFVDPSPGVSPLDLLGSVSRGLYAAVLLERPDVDLAADRFRIVVAGYAVE
                     KGRATERVSEAAVSGRLSEFLRGVSGIGDDLKFAIGGTGSIGSPTLLVPRWKLG"
     gene            complement(8379..9737)
                     /locus_tag="P5164_00300"
     CDS             complement(8379..9737)
                     /locus_tag="P5164_00300"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HRY42356.1"
                     /translation="MKELPELARDADDAVRLVSGLLRRGETGEAFRERVRTESWSATE
                     SGLLAPALSVESGTAVRIRREGRLLLVARSGAGPEALRDAVREAGRLAGNAPFFKAHR
                     TTGAAPGGRDAERPEEEESRSAALAAALARAFPDPRGVTAALQLSRVVTERTVVTPRA
                     FLPCGARTRLVATGTIRRAGAERPFSFQSARPFAEAADALALALREAIRPVPALPPQP
                     GPVDVVFSPSAASVFWHEVVGHSLEADGGERGSVLSRVKGAAVAPPGLDVHDDPGRAD
                     LPGAYGVDDEGSLARAVPLLAGGVVTGLLSDRRSGAVETNGHGRTASFRRPPRARMSN
                     LVANAGSATLDELVARCGDGILVKEVATGSADPESGRFVLFVAAAEAIRRGRRNAPLA
                     PFALGGEILSALGAIEEVWGNEVLPATGLSVCVKGGDAVPVGGAAAALLVRGLFARTA
                     RR"
     gene            9803..10081
                     /locus_tag="P5164_00305"
     CDS             9803..10081
                     /locus_tag="P5164_00305"
                     /inference="COORDINATES: protein motif:HMM:NF016271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HRY42357.1"
                     /translation="MAAPDARLAACAAFVARRLLDSGLVKAGSGAPVRKAVESVLVFD
                     RDRERDLEAEVEKLLRAHGAATLGAGVDYAEMFRKAKRLLAEKKKIPL"
     gene            10098..11657
                     /locus_tag="P5164_00310"
     CDS             10098..11657
                     /locus_tag="P5164_00310"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRY42358.1"
                     /translation="MRTIEERIRHLDGLRRAARLGGGEARLKKQHEAGKLSARERVEL
                     LGDPGSFEETDPFVVHRCRDFGMDAEGNTVPGDGVVAGAVRVEGRPVFVFAQDFTVFG
                     GSLSETNAAKVCKVMDLAMRAGAPVVGLNDSGGARIQEGVASLAGYADIFLRNTLASG
                     VVPQLSAIMGPCAGGAVYSPAITDFVLMVRGTASMFVTGPDVIKSVTHEEVTKEALGG
                     AETHASVSGVAHVVADSDADCILKIRTLLGFLPSNNRQDPPRRESADPCDREDPELDT
                     LVPASPNQPYDMKDVIRRVVDDGELFEIHADFAKNLVVGFARLGGRPVGIVANQPDFL
                     AGVLDIDSSTKGARFVRFCDAFNVPLVTFEDVPGFLPGTQQEFGGIIRHGAKLLYAFA
                     EATVPKVTVITRKAYGGAYCVMSSKHIRTDFNYAWPTAEIAVMGPEGAVNVLYGRELA
                     RSADPAAARKAKVEEYQDLFANPYVAAQKGYVDDVILPRTTRRRLIRALDLVENKRDV
                     LPPKKHGNMPL"
     gene            11848..13332
                     /locus_tag="P5164_00315"
     CDS             11848..13332
                     /locus_tag="P5164_00315"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRY42359.1"
                     /translation="MRLLIANRGEIAVRIARACRDLGIEPVAVFSDADRAALHVRLSD
                     HAVGIGPAPARESYLVPEKLIDAAKRTGARLVHPGFGFLAENAAFARAVRDAGLVWVG
                     PDPESIAAMGSKTESRARMQAAGVPIVPGMTSPAKDPAEIAAFGRKHGFPLLLKAAAG
                     GGGKGMRVVRSEEEVPAAFERARSEARSFFADEAVYCELYVERPRHVEVQVLGDRTGR
                     VIALGERECSLQRRHQKVLEECPSPVVTPELRARMEESAVAAARSVDYVSAGTVEFLL
                     ASDGRFFFLEMNTRIQVEHPVTEMVYGVDLVAEMIRIALGEPMSVAARPEPRGHAVEC
                     RIYAEDPAQGFAPSPGTIERLRWPQGPGIRVDSGVEEGDTIPLDYDPMVAKVVTHGRD
                     RDEALRRMRRALRETRVDGIETSIPFFLELLEDADVLSANVSTQFLESFAFEPSVPSA
                     DAEEIGLVAAAVAAARASLARRGASQPRPPSAWRTARPTYGHRE"
     gene            13329..13856
                     /locus_tag="P5164_00320"
     CDS             13329..13856
                     /locus_tag="P5164_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRY42360.1"
                     /translation="MKYRAKLTGPAGERTVEVDVQLDPAGVVVTLDGRPARWDVAPTP
                     GGGASLLRADGRQAEAVPGRSAEGAVLVRVGAERVALELLDELTARAQAVAGAGSARG
                     SGDVKAAIPGRVLRILVAPGDEVTRGQPVVVLEAMKMENDVRAPRDGVVASVEVAAGQ
                     PVGAGQLLLRLEPAV"
     gene            14002..15216
                     /locus_tag="P5164_00325"
     CDS             14002..15216
                     /locus_tag="P5164_00325"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRY42361.1"
                     /translation="MKHYRSLVLFAATALLACRAGGTPQSAAPEKPAVTPAASPAPAA
                     SPAAPAPAAKVADPRTEALVAWLGEYFPWGPGELKVEPLDAVRVPGWRLYRATKTFEG
                     DQRANDQAFLLADDAGKTVLAGDIFVEEERLRAPKPVRGPEDVGALRELLARFLRGRF
                     DVVFEPSRDLPSFKGLTIKHETGYGSYDIGAYVTSGDGAVLLVGRAWERAKSIAAQRR
                     ALIRVDGAPTSGPADATVTIVEYSDMQCPFCKKRAADLDALLAKMSKELKVRRVSKSF
                     PIADHAWAFRASSAGVCFADFKKGLHAYWKSAVFARQETLSVPELDTFALDFAVANGM
                     SEEAFRACYLQGGAVTKVLDDLAEGFAVRVRSTPTYFVDGVPLSWFSDPLMEDYLRKT
                     YLKGAASPAAAK"
     gene            complement(15311..17185)
                     /locus_tag="P5164_00330"
     CDS             complement(15311..17185)
                     /locus_tag="P5164_00330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRY42362.1"
                     /translation="MSSSGPVPRIFGPFVLTRHLGTDPLGGLYRAGTATGARLAPFLL
                     IRVFEGAGISQPSLVPVMEEASEYLEVVRGPAVAKGAVLGIVDDVPFAGIEYVPGRTL
                     DRLFSGEGQGAIPLPVEHALLIAEKLLVALEAGKPFEKKMGAPHGFLTPGFVTISNDG
                     DVRVYGAGLGSGLLPSLKSAPIRSAFAPYIAPEVLASGKPTVAGDIYSAAAILFHALT
                     GKPPAPGAGADSLAGAVLAIDGSPMPDDLGKLVARGLAKDPAQREQDVVAYRKTLGKL
                     LYGGPYAPSTFNLAFFMHQKFEKAIERERIELGQEETIDPKPLVAAEEKKDKDARLKA
                     QAARAAASIPVPSFGGGTSPGTTTLGGTPVAKKGGLGGIPVPVVAGGAVVVLGLAGWL
                     LLGRGPKQPAAPPPTPVPVATPTPMPAPTPVIVGKEDPEFQAALQAKLQEEMKKVQDQ
                     ISKEQAAATKRRADEQAALEAAAAKAREAEEAVRAARERSDTAEAERLAKEAQEARAR
                     EESARKAAEAAVPKVKAGDLLEIGQVTKQPRAVKVVKPEATSLARQKKVSGTVLVRVL
                     VDEKGRPAEVEIYRDTNPKVGLGEASLAAVKKWEFTPAEKDGVPVKTWVVVPLPFVVR
                     "
     gene            complement(17232..18287)
                     /gene="mltG"
                     /locus_tag="P5164_00335"
     CDS             complement(17232..18287)
                     /gene="mltG"
                     /locus_tag="P5164_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRY42363.1"
                     /translation="MRRLLRIGLRGLGLLALVAALGAGAALFLVRSPYQGWTGGEVVV
                     ELPSGASARSILARLERAGVIRNRHLAGAAYRVLFEGKTLKAGEYRFTRPVSTEEVLR
                     DLVEGNVVTHRLTIPEGWTAEEIFALLEREGLAPAEPLRELFLKPALFPEVPPDAPTL
                     EGFLGPETHLFTRSQRPREIVATLVKAWAHSLPEGFAERAAALGRTPLEAVTLASLVE
                     KETGVARERPLVSAVYHNRLRVGMPLQCDPTVVYALKRRGLFAGGLTRASLAVDDPYN
                     TYARGGLPPGPIASPGAAALEAAVAPAGVPYLYFVAVGDGSGEHRFAVTYPEHLENVA
                     LYRRARTARETVFGASR"
     gene            complement(18284..18712)
                     /gene="ruvX"
                     /locus_tag="P5164_00340"
     CDS             complement(18284..18712)
                     /gene="ruvX"
                     /locus_tag="P5164_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014464891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRY42364.1"
                     /translation="MRILAVDFGERRIGLAVSGPAGAVVVPVGVVHRKSDPQAVSAVA
                     AAAREREAALVVVGLPVRPEGGGESPFAARARSFAKKLAAATGLPVELHDEAFTSVSA
                     EGALREAGLSRDRRSGAVDAEAAAELLRDWLSSHPDGARG"
     gene            18843..20027
                     /locus_tag="P5164_00345"
     CDS             18843..20027
                     /locus_tag="P5164_00345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HRY42365.1"
                     /translation="MKNVLVLSATRTPIGTFLGSLAPLTASEIGARALKSCLERTEVP
                     GYRVDQVLMGNVLQAGQGQAPARQALIAAGLPKSTGAVTIHKVCGSGMRAVMDGANAI
                     RAGESEIVLAGGMESMSNAPYLLQKGRTGMRMGHGQLIDGMIHDGLWDPYKNVHMGNC
                     AEMCAARYSFTREAQDAFAMESYQRARRANDSGDFRAEMVPVSIEGKKGPVVVDRDEE
                     PFATPLEKLEKMGSLKPAFQKDGTVTAANSSKINDGAAALLLATEAVAQETGAKPLGR
                     IVSFASHAHEPEWFTTAPAFAAKKALEKAGIPLSQIDLFEVNEAFAVVTMAFVKDLEV
                     DPNRVNVNGGAVAMGHPIGASGARILVTLLHALKARKKKYGLAAICIGGGEATAMVVE
                     AL"
     gene            20024..20737
                     /locus_tag="P5164_00350"
     CDS             20024..20737
                     /locus_tag="P5164_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014464931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HRY42366.1"
                     /translation="MIDKVVLSSSHHEAAARAVADIPSGATIAVGGFGLCGIPESLIQ
                     ALADRDVKDLVCVSNNCGVDDWGLGILLGKKMIRKMVSSYVGENAEFERQYLAGELEL
                     EFCPQGTLAERLRAGGAGIAGFYTPTGVGTVVAEGKETKLFDGREYVLERGIVTDYAL
                     VAAWKGDRHGNLVYRKTAKNFNPMCATAGKITIAEVEQLVDVGEIDPDEVDTPGVFVN
                     RLIVAPRAKRIERRTVRKG"
     gene            20748..21413
                     /locus_tag="P5164_00355"
     CDS             20748..21413
                     /locus_tag="P5164_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HRY42367.1"
                     /translation="MAKLERDGIVARAAQELRDGMYVNLGIGMPTLVANAVPPGMEVV
                     FQSENGILGMSGFPTEAEVDADLINAGKETVTVIPGASFFSSADSFAMIRGGHIDLCI
                     LGAMQVDAEGSLANWMIPGRLVKGMGGAMDLVAGARRVIVTMEHVAKKGDKKILNRCT
                     LPFTGKRCVHRIVTDLAVLDVKPDGAGLVLVERAPGVTVEEIQAATEPPLEVLGDVPE
                     MKL"
     gene            21731..23623
                     /gene="lptD"
                     /locus_tag="P5164_00360"
     CDS             21731..23623
                     /gene="lptD"
                     /locus_tag="P5164_00360"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HRY42368.1"
                     /translation="MRADTKTKQVVAEGDVVLEQGTTRLKGLRLEMDLTDKTGILTDG
                     SAELAGILVRGATLAKTGARTFEIRDGSVTACEGDDPAWEFRVKDGAVTLDDYAKLKS
                     VVFRLGGVPLLYTPYLLWPALRDRASGFLVPGIGYSTSRGGFLGLSYFQVLGRSADAT
                     ITTDLYTKEYFGLGTELRLRPTAGTAFTGSFYTVVDPDRRWQWKTWGTLSADDLAPRT
                     RAVVNWTDFSDLAFFQRYDRDFALTSARTVGQKAFVTRSEGPVAFNLRLERETALYGS
                     SEVVLERKPVLEARLRPTGLFGNALFVEAQGSAGFLRSIRPAGQPAGDYERFDLYPKL
                     SAPLSPLPWLSLNAEAGYRVTRYGASVSADGRSLTEEPYSRGSATAGLEVTGPSFSRV
                     FDVKLGKFRKLKHVIEPRADYSYQTDPEDLSRTPVFDEVDTILPSHQVRYALVQRLLA
                     KEEKGASREIASFELSRTHYFELPVGAATTVRDSPLDAVLRVNAGSALNFDARATWDL
                     KEGVVTSSSVSANVGSAERFLRLSFFDSRPAGAPTPSTQLRFGGGSSLLPKRLRLDVE
                     ANYDVERGKMLEWRSLLTWEASCFKILGEYRDLRIGAIPTRDYRIGLNLKNVGSFLDF
                     TGRVGN"
     gene            23661..24506
                     /gene="rfbD"
                     /locus_tag="P5164_00365"
     CDS             23661..24506
                     /gene="rfbD"
                     /locus_tag="P5164_00365"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HRY42369.1"
                     /translation="MNELILGAGGMLARGLAALRPRARALGIAELDVTDAAALARVIV
                     PGVSVVWNAAADTRVDLCESDPGHLAVNDVAVGEVARLCRDAGALLVHVSTDYVFDGR
                     PGRPWREDDPVDPVNAYGRGKLGGERRALASGAEVLVVRTSWVFAPGGVNFVDTILGL
                     AESGREELKVVDDQRGRPTYAPDLARALVKLVGSGARGLVHFANAGETTWYGLAKEAL
                     ARAGFAGVRVLPCASDEFPRPAARPANSVLDTSRYEEMTGEAPRHHLEALDEHLRVRA
                     ETGRA"
     gene            24503..25840
                     /locus_tag="P5164_00370"
     CDS             24503..25840
                     /locus_tag="P5164_00370"
                     /inference="COORDINATES: protein motif:HMM:NF014049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HRY42370.1"
                     /translation="MSLDATEARRKAVHVGMASFALLLRWLDVRGAALCALGAFAFNL
                     LVLPRLGGRSLFREGDRARGYPVGILLYPLVVLALVLLFPRRLDLAAAGWGFLAFGDG
                     FATLVGASLGGRRLPWNPRKSWSGLLGYVVFGSLGAAALSSFVAGRLPAPLEHAAFLL
                     AALVGAVLESLPSELDDNVVPPLAAAGALALLLPALPNAALLLEPEALRRLAAGVGVN
                     VALPALAAALGVVRPSGAVAGGIVGALVLGLGGFGPYALLWAFFAGGTLATRFGRARK
                     EAMGKAEAAGGRRSAANVLANCSVAAFLVACAALTPAASGLLHLAAAAAFATALMDTI
                     GTEVGQAIRTPTVLLPDLRRVPPGTDGAVSIAGTLAGLLAAALLAALGALLDLYALAA
                     LPAVVAAAFAGTVVESLLGRDGASWRVTNGHVLNLYNTLAGAAVAALIARFAL"
     gene            complement(26022..26420)
                     /locus_tag="P5164_00375"
     CDS             complement(26022..26420)
                     /locus_tag="P5164_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005199352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HRY42371.1"
                     /translation="MKDSPVALQTTTSLSFADAVARAREELAKEGFGVLTEIDVAATL
                     KKKLDVDREPYLILGACHPPSAHKALSAVPEVGVLLPCNVTVSVEDGTTVVRAMDPAG
                     VMGILGSEELAEVGADIGARLRRVVDAFRA"
     gene            complement(26507..26707)
                     /locus_tag="P5164_00380"
     CDS             complement(26507..26707)
                     /locus_tag="P5164_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRY42372.1"
                     /translation="MPLYEYSCDECGRTFEELRPSSEADAAIDCPSCESRRTSRKLSS
                     FASGASSSGSPKGGSGCGTRFT"
     gene            complement(27197..27571)
                     /locus_tag="P5164_00385"
     CDS             complement(27197..27571)
                     /locus_tag="P5164_00385"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRY42373.1"
                     /translation="MVMRKAASLPKEQLMEGIASFLKVLADPTRLRILHALQGGERCV
                     SDLLAELPCTQANVSKHLALLRTAGIVEFRRQGTQVYYRIADPAVFAICGVVCGAMQR
                     SLDERRVELKAGKAALAAGARA"
     gene            complement(27688..27972)
                     /locus_tag="P5164_00390"
     CDS             complement(27688..27972)
                     /locus_tag="P5164_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42374.1"
                     /translation="MRMLMIVVDDGKKEELEVLLRKSGVTGWTEIAGASGMGTTGPRL
                     GSAAFPKTSAVLFTIVEDERARALADEIKAYCAECGERLRIASWAVEELL"
     gene            complement(27960..28166)
                     /locus_tag="P5164_00395"
     CDS             complement(27960..28166)
                     /locus_tag="P5164_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRY42375.1"
                     /translation="MNLNEWLRAIAGFFVLLSVVLAVTVDQRFLWFTGFVGLNLLQSA
                     FTKWCPAIAMLRKLGVKEGGACGC"
     gene            complement(28214..31465)
                     /locus_tag="P5164_00400"
     CDS             complement(28214..31465)
                     /locus_tag="P5164_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRY42376.1"
                     /translation="MTRRPVGASGRIAKAFLESKLTPLLVVASLLVGAFAVMVTPREE
                     EPQIKVPMIDVFLGMPGATAREVERRLTSPLEKAIYEIPNVEYVYSITQPSGGMIIVR
                     FLVGTDPDQAAVRVHTKLAEKASELPPGAMPPLVVPRGIDDVPVLAYTLSSADASPMQ
                     LKEVAEELKVELTKHPRVAQVTVLGGEKRAVKVAFDRDRLAAHQVSLLQAYMALSGAN
                     WRLPAGSFATSDSETQVEVGSLFRSAEEVGNAVVAVWNGRPVFLRDVATVTDGADEPS
                     QYVWMLSGPGAAHRGLAPGTDTAAVTVSVAKKPGTNAVELVEELDAKVAGLKGKVVPS
                     NVTLTKTRDYGHTADEKSNELILHMALATFSVVLLMAFALGRREAVVVLVAVPMTLAL
                     TLASSYLFGYTLNRVTLFALIFAIGILVDDAIVVVENIHRHYELGWGPPRLTTVYAVD
                     EVGNPTILATFAVVGALLPLVFVSGLMGPYMAPIPINASAAMIFSLFVAFVVSPWLTF
                     KLFRKYAEEHANRSAFESHAETPQEDKLHRFYQRLFEPLLTSPAKSWALIGGVVVLLL
                     LSTSLVYFKVAKVKMLPYDNKSEIQVVVDMPEGTTLEATNAVARDLALEVRRQPEVTD
                     VQVYVGTTAPFNFNGLVRHYFLRSGPLVADLQVNLLPKQERKRDSHTFAKDVRTLLLP
                     IAKRHGANLKVTEVPPGPPVLSTMVAEIYGPDMAKRIDLARQVRTIFEETPGIVDVDW
                     HVEDEAPKVELVVDREKATRAGVTPEVVTRTLRVALNGADAGLLRDESSRNPVPIVLR
                     LERAQRSSLDGLLQTAVHGGTGRLVPLRELVTVKETKSERFIYHKNLQPVTYVLAEMA
                     GVAEAPVYGILDMGERIGSLKAPDGSPLQVMSTTLPTDSTKYAMKWDGEWHITYEVFR
                     DMGIAFAAVLVLIYFLVVGWFRSFVTPLIIMAPIPLTLIGILPAHGLFGVFFTATSMI
                     GFIALAGIIVRNSILLVDFINLELEMGEKLEDAVIKAGAVRFRPIALTAAALVVGGGV
                     ILLDPIFQGLAVALIAGVVVATALTLVVIPLLYYMYLKAVGVEKALPPQDEAA"
     gene            complement(31570..32667)
                     /locus_tag="P5164_00405"
     CDS             complement(31570..32667)
                     /locus_tag="P5164_00405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY42377.1"
                     /translation="MTSRKTVSLVTLTVAGALALTACGGGHKAGDVATPDPVSASLAK
                     AESRELPQRIELSGTVEAGKSAMVSSRVMASVVAVPVKEGDLVAQGQLLVEIDPQTAK
                     GQESQARGALAQAKAALALAERNWQRFQALQKSAAASELEVDMARMQYEQAKGAVEQA
                     TGAVEAASSVARESRVVAPFAGRVARKMVDPGDLAAPGRPLVVVESTAGRRLVLSVPE
                     SVAVASKLKTGALLPVSLDAMPGATLQGRVVEMSAGADPASHSFTARVEVSGAQVPTG
                     VTGRALLETGRRTAVVVPAAAVLRQGAMSLVVVKDEEGKARTRAVTLGTRDGDLVEVL
                     SGLKGGEDVLVGLAQAPADGAPVNASSEVVP"
BASE COUNT         4427 a        11798 c        12280 g         4379 t
ORIGIN      
        1 tggctcctcg tccaccaggt cctcccgttc gtgccgctcg ccggcgccgc gtgggccgcg
       61 ctctccgcct cgcggaaacg ggcgctcgcc gtcgccgcgc cggccttcgc ggccacgctc
      121 gtctcggccc tctcgctctc gttctggaac ccgctcctct ccccggcgca cccgcgggcg
      181 gagtcggtcg tcttcacgca gacgagcccc gaggtgatgc cggtcgtcgc ggagatcctc
      241 agggccgggg gctcgggcga agatccggcg gcggcggtct cgggagaggc cgtctggccc
      301 ctgtcctggt acctgcgggg actccccgtc ctctgggcgc tcccgtccgc ggggacgcgg
      361 ccgccggtcg tggtcgtcga cgagggaaag gtggaggagg cgcagcgcct cctcgggggc
      421 ggctacgcgg tcgagacgat cccgcttcgc tcctggtggc tcccggaggt ctccctctcg
      481 ccgctccgcc cgaatgcccg cgagctcctc gtctatctct tcacgcgccg tccgtggagc
      541 cccgtggggg cgcagaacgt cgtcgtcctc cggcgtccgg acggcgggag gccgagccga
      601 tgagcgcacc ggagatctcc gtcgtcctcc cctgcttcaa cgaggtcgag aacatccccg
      661 agctccggcg ccgcctcgtg gcggtcctcg aggcggccgg gcggagcttc gaggtcgtct
      721 tcgtcgacga cgggagccgc gacgggtcgt gggaggcgat ggcggcgcag gccgccgagg
      781 acccgcgcct ccgcgcgatc cgcctctcgc ggaacttcgg ccaccagatg gccctgacgg
      841 cgggcgtcga cggggcgcgc ggccggctcg tcgcggtgat ggacgccgac ctgcaggacc
      901 cgccggagct cctcgccgag atgctctcga aggcggacga ggggtacgag gtcgtctacg
      961 cgcagcggtc ggcgcgcgag ggggagagcg ccttcaagct cgcgacggcg caccttttct
     1021 accgcctcat gcgccgctgg acgaacgtcg acatcccggt cgacacgggc gacttccggc
     1081 tcatgggccc gaaggcggtc gcggccttcc ggtcgctgcg cgagaggcac cggttcatcc
     1141 gcgggatgac ggcgtgggtc ggcttccggc aggtcgcggt ctcctacgtg cgccccccga
     1201 ggctgcacgg cgagacgaag ttcccgctcc ggcggatggt ccgcttcgcg ctcgacgcgc
     1261 tcacttcctt cagccacgtg ccgctccagc tcgggacgtg gctgggtttc ggcgtctcgc
     1321 tcttcgcgtt cctctacatc ctcgtcgtcc tcgtcctgaa ggtgctcggg atcaacgaac
     1381 ggggctggac gacgctgatg gggtggatgc tcctcctggg cggcgtgcag ctgatgctca
     1441 tcggcgtcct cggggagtac ctcgggcgga tctacgacga ggtgaagcgg cggccgctct
     1501 acctcgtctc cgaggaggcg ggaggcccgg agagggacac cgcgccggag gcggacccgg
     1561 cgcgctgagc ccgccccggg acgggggccg gtgctatcct gtcccctccg gaggtaatcc
     1621 catgcggcgc gtactctcgc tgctcgtgct cgggctcatc ggcctcctct ccgggccgct
     1681 cgcggccgac caggtcgtcc tccgggacgg ccgcaccctc gacacgaaga agccgcccgt
     1741 gatcaagggc cgccaggcgg tcctcacgct cgcggacggg aagctcgtct cgatccccgc
     1801 ggccgagatc gaccaggaga agacggcggc cctcgccaag aaggcggccg aggcgccgaa
     1861 ggcggcgccc gccgagacgc cgcgcgcgcc ctccctcgtc gacgccgccc gggcgacgca
     1921 gaacgcccgg aaggccgcgg tcgtcctgac cgaccaggac gtcgcgggcg ggtacctcga
     1981 tgtttcctcc ggctcgcccg aaaagggcga gggggaggtc tcgatcgggc cgacgacgac
     2041 gaagaagagc gacgctggct acgtcatcga gggctccgtc ctgaactcgg ggaaggcccc
     2101 ggtcctcggc gtgagcgtga cggtcgaggc ggtcggggcg gacgggaagt cgatcgcgac
     2161 gacctacgcg gccctcgcca aggacgttct cgacccgggc gagaaggcgt cgttccgggc
     2221 gcagatcgac acggacaaga cggtcgagcg gttcggctac gtgccgcgct ggaaagtgat
     2281 cgcgccgccc gcgaaggagg gcgagccggg cgaacgggcg gagcagaaga aggagcccgg
     2341 cgcggcgggc gaagaagcgc ctccgccgcc gccccctccc gcgaaggagg aagcgccgcc
     2401 gcagcgtccg cccgacatgg ccgctccgcc ggcgaacgct ccggtcggcg ccccgtcgca
     2461 gccgggcggg tcgtacctgc cgcccccctc ctcgaaccag ccgaagccgc ccggcggccg
     2521 gggctgacgg gggccatacc cgggccggga tggccgccga gatcctcctc gtcgaggacc
     2581 gggagtcgct ccggtcgatg ctcgccgaga cgctcggcgg ggaggggtat cgcgtcgagg
     2641 cggtcgccgc cggggacgag gcggtccgaa ggctctccga ggggcggcgc tatgcgctcg
     2701 tcctgaccga cctgaagctc ccgggggcgg acggcctcgc ggtcctggag tccgctctcg
     2761 cgaacgaccc gtccgtcccg gtcgtcctcc tcaccggcta cgggaccgtc gagacggccg
     2821 tggcggcgat gaagaggggc gcggccgact tcctgaacaa gccggtcgac gtcgacctgc
     2881 tgctcctcct cgtgaagcgt cacgtggagg cgcgccggag cgccgtcgcc cgcgcgctcc
     2941 tcgcggagga ggcggggctc gccgggatgc cgcgcatcct ggggagctcg cccgcgctcg
     3001 cggacgctct cgaccgcctc cggcgcgccg ccccgagcga cgtgacggtc ctcctgacgg
     3061 gggaatcggg gaccggcaag gagctcttcg cccgggcgct ccacgcgctc tcgccgcgcc
     3121 gcgccgggcc gttcgtcgcc gtgaacgtcg ccgcgctccc cgagggtctc gtcgagaacg
     3181 agctcttcgg ccacgagaag ggggcgtaca cgggggccgg agagcggcgc gccgggcgct
     3241 tcgagctggc cgacggcggg acgctcttcc tcgacgagat cggcgagctg ccgctcggcg
     3301 ctcagacgaa gctcctgcgg gcggtcgagg agcggacgtt cctccgcgtc ggcgggaccg
     3361 tcccgctctc cgtcgacgtg aggctcgtcg tcgccacgaa ccgggacctc gcccagcggg
     3421 tgaaggcggg ggcgttccgc gaggacctct actaccgcct cgacgtcttc ccggtgaagc
     3481 tgccgccgct gcgcgcccgc agggaggacg tcccggagct ggcgcgggcg ttcgcgcgga
     3541 gctgcggcga aggggcgcgc gggagggcgt tcgagatcgg cgccggcgcg gccgagaagc
     3601 tcgcggcgtg gaactggccg gggaacgtgc gggagctgaa gaacgtcgtc gagcgggccg
     3661 tccttctcgc ggaggggtcc gtcatccggc cggccgacgt cgtcctcggc gctccgggcg
     3721 aggacgcgcc gaccacgccg acgctcgacg ggacgctcga ggaggccggg gagcgctgga
     3781 agcgcgcggg cgaagccgcg cgcatccgcc gcgctctcga ggaagccggg ggcgaccgag
     3841 ccgccgccgc cgccgccctc gacatcccgc tcaagcgcct cgcccagcgc ctccgcgacc
     3901 tgaagctcta gacctcgctc cccgcctcgc gaagggcgga ggcgaggtcg gcgtcctcgt
     3961 cgggggagac gctccggagg tcgaggacga ggcggccgtc ggcgacgcgg gcgaggacgg
     4021 ggagctcgcg ggcgcggagg cgggcggccg cgtcgccttc gccgagggac gggtgccgga
     4081 ggaggacgcc ccacgacgga agcgtcgact ccgtgccggc gccgccgccg gcgatcgcga
     4141 cgagcggcgc ggcctcggcg ctccagccgg cggcggcggc cacggccgcg aggcgccgcg
     4201 cgcgcgcctc gagatcggcg agcggcgccg cgagcatcgc gtggaacggc acctcggccg
     4261 cgcgccccga gaggtggagg tcgagcgtcg ccgcgagggc ggcgaggacg agcttgtcgg
     4321 ggcggagcgc gcgggcgagc gggctgcgcg cgcagctccg gacgaggtct tcccggccga
     4381 ggaggatccc cgcctgcggc cctccgagcg tcttgtcgcc cgagaagcag acgaggtcgg
     4441 ccccctcggc gagggcctcg gccgggacgg gctcgtcgca gaggcccggc tcggcgaacc
     4501 tttcgggcga gccggcgccg gcgtcgtaga ggagcgggac gcccgcttcg tggcagagcc
     4561 ccgcgagctc gggaagcgcg acctcctccg tgaagccgac gaccctgtag ttcgaggggc
     4621 gcactttcag gaccgccgcc gcgccgacgc gaagggcctc gacgtagtcg tccggcgtcg
     4681 tcttgttcgt ggtgccgacc tcgaggagcg acgccccgct cttcgcgagg atcgaaggga
     4741 cccggaagtc gcccccgatg gcgacgagct cgccgcggga gacggcgacc ggccggccgt
     4801 tcgcgacggt gtcgagcgcg aggaggagcg ccgccgcggc gttcgtcacg gcgagggcgt
     4861 cgacgcccgg ccggcccggg gcgaagagcg agaggaggag cggccggacg tgctcgccgc
     4921 gtcggccgcg gccgcccgta tcgaggtcga gctcgagcgt ggagtagccg gcggcgcgca
     4981 ggatcgcctc gcgcgccgcc gtggcgaggg gcgcgcgccc gaggttcgtg tggaggagga
     5041 cgccggtggc gttcaggacg gggcgcggtc cggcgggcgg ccgggccgcg agcgcggccg
     5101 ccgcgccgtc gaggagcgtc gcggcggggt cggcgcccgg ctcgagcgtt ccggacctga
     5161 ggcgggccgc gccctctcgc aggagctccc ggacccgcag ccgaccgtgc cgcccggcca
     5221 gggcccggcc cctctcgctc gaaaggagcg cctcgatcga aggagggagg acggccggtc
     5281 tcgccaagac ccttagaatc tagcgcactg atggggatct cgatcgaacg ggagctggtc
     5341 ttcctcgagc gaaacatcac ggccctccgg gtcgagtacg agcggttctt cggcggagag
     5401 ctgaagaggc cgccggtcca gtcccggcgg acgatcgagg acgtcctccg ccgggccggg
     5461 aacgccgagg tcgagaaggc cgcggaacgg ttccggctgc agtcgctcca gagccgctac
     5521 aactccttcg tcgagatctg ggacaagcgg atgagggcga aggaggaggg gacgacgggg
     5581 ccggttcggc ggctcgcgtc gagcacgccg gccgtcaagc ccgaggcgcc gcccgcatcc
     5641 acgaagcccg cggcgaagaa ggccgattcc gccgccgacg tggcggccct cctcgcggcg
     5701 gccgaggccg ccgtcgcccg gaagcagcct ccgccggccc cggctcccga cgctccggcc
     5761 gcgccttccg taaaaccgaa gagccgggtc gatttcactc ctctcttcca gaggtacgtg
     5821 gcggcccggc aggccctggg agaggacgtg acgaagctgc gatacgagaa gttcgaggag
     5881 gcggtgcggc ggcaggccga ggagatccgc aagcggaccg gctcgtcgcg gctcgtcttc
     5941 gaggtccaga cggcggaggg acgggtgaag ctcgtcggcc ggccggtccc ggctcccggg
     6001 tcgaaggggt gaagatgctc gcaccgtttc cgaaggcgct cctcccgggc gcgatcgccg
     6061 tttcccttct cctcgcgccc gtcgcggcgg ggaaggacgt ctacaggccg tacctcgaca
     6121 aggcgatccc gcaccacgcg gcgatcctcg acacgctcga gaagctcgag aagagccccg
     6181 aggacgcgca gctccacaac gacctcggct gcctcgtcgc gtgggacggc ttctggaggg
     6241 acgccctccg gagcttcgac gaagcctccg acctcgaccc gaaggactcg cgcccctggt
     6301 tcaacgcggg gctcgtccag gcgctccggg gcgagtgggg cgcggcgcgg tcgcgcttcc
     6361 ggaaggccgt gaaggtcgac ccgggcaact ggtcggcctg gtggatgctc ggattcgccg
     6421 aggagacgct cggccacgag agggcggcgg tcgaggccta cagccgctcg ctgcgcgtcg
     6481 acacctcgct cttcgacccg cgcgtgaacc cgttcgccgt cgcgacccgg ctgaagtcgc
     6541 gggtcctcct ccggacctac gagcggcgcc gggtcgacgc cgccctcccg ttctcgaacc
     6601 agctctccga cgcgtcccgc ctcgccgcct tcttccaggc gagcccggcg ccggcgaaga
     6661 gcgccgcccc ggcccccgag ccggcgcgaa ccggcccggt cgttacggcg gtgccgtcgt
     6721 cgacggtcct gccggacgca gcggcctcgg agccggcgca gacgcgccct tcccgccgcc
     6781 ggcgcgcgta tccgagggga agcgacccgt cggagcccgt cgagctgcag ccggagccgc
     6841 ctcgccagga gcccgagccg gcggccgccg agccgaagcc cgagggcggg gacgctcccc
     6901 gcacgcccgg accgggcggt ggcgggccgg gcctcggaag cggggcccgg cgcgttccgg
     6961 gccccggtgg ggcgcctccg gccgagccgc agcccgagcc gcagcccgag cccagatagg
     7021 cggcccggcg ccgccccggc gtcagccgag cttccagcgc gggacgagga gcgtcgggga
     7081 gccgatgctc ccggtccctc cgatggcgaa cttcaggtcg tcgccgatgc cggagacgcc
     7141 gcgcaggaac tctgagagcc ggccggagac cgccgcctcg gagacccgct ccgtggcgcg
     7201 ccccttctcg acggcgtagc ccgcgacgac gatccggaac cggtcggccg cgaggtcgac
     7261 gtcgggcctc tccagaagga ccgcggcgta gaggccccgc gagacgctgc cgaggaggtc
     7321 gagcggcgaa acccccggcg aggggtcgac gaagaagttc gtcacgccga tccgcggcgc
     7381 gtcgctccag gcgacgcgga tcgcgttccc cgagaggggg cgcgagagcc gggacgccgc
     7441 ggcggagtcg gtcaggcgcc ccgacggaag gccgcgctcg acgaggcggg tccggccctg
     7501 gggagtccct tctccgtcgc ggacgccgcg cacgaggccc cccgagacgg tcgcgtcgtc
     7561 gacgaccgag aggagcggcg aggcgagcgc gtcgcgtccg tcgcgcgtgc gggccgcgag
     7621 gaggacttcc tcgtcgtcac cgaagaagag cggcgcgagc cgccccacga ggtgagcggc
     7681 gaccaggggg tcgaggagga cgtcggcgcg cgccgcctcg aacggccggc cctggagcgg
     7741 gaggaggacc cggtcggccg cgtgccgggc gagccgggcg accggaagct cctccgggcg
     7801 gggcgcggcg ccgacgacgc gggccgagaa gcggccgccc gtcgaacggc cgaccacggt
     7861 ggccacgagc gtcgcgaggc ggctccccca ggccgtgtcg cgtcccgagg acgtcgcgag
     7921 gcgctccgtg cgctctccga gcgagacaga ggcctcccgg accgcgacgg atccgcggcc
     7981 ggagctcgtg agcgcctggc ggaagccggt caggagcgtc cgggccaggt cgacgttcgg
     8041 ggcgggcggg tcgccgatcg ggcgcggctc gagggcctcg cccgcggccg gggccgggag
     8101 cctcacgccg cggcgcgtac gggcccgcgg caggcgcgcg cgcgcctggt cgggaaggga
     8161 gaaggcgtgc gcgaggccgg aggcggcgaa cgccgagcgt ccccccttga agagccggag
     8221 cgcgagcccg cgctcgcccg actcgaggag ggaggtgccg gtctcgtcct cggtcagctc
     8281 gagcagccgg cctcggcgct cgtacagctc cgccccgcag ccgtcggtcg agcccgcttc
     8341 gaggaccctc tcgaagaggc cgtcgtcgga cgcgggggtc atctccgcgc cgtcctcgcg
     8401 aagaggccgc gcaccaggag ggccgccgcg gccccgccca cggggacggc gtctccccct
     8461 ttgacgcaga ccgagaggcc ggtcgcggga aggacctcgt tcccccagac ctcctcgatc
     8521 gcgccgaggg cggagaggat ctcgccgccg agggcgaagg gagcgagcgg ggcgttgcgg
     8581 cgcccgcggc gaatcgcctc ggcggcggcg acgaagagga cgaagcgccc cgactcgggg
     8641 tcggcggagc cggtcgccac ctccttcacg aggatcccgt cgccgcaccg cgcgacgagc
     8701 tcgtcgaggg tcgcgctgcc ggcgttcgcg acgaggttcg acatccgggc ccgcggaggg
     8761 cgccggaagc tggccgtccg accgtgcccg ttcgtctcga cggctccgga ccgccggtcc
     8821 gagaggaggc ccgtcacgac tccgccggcc aggagcggaa ccgcgcgggc cagagagccc
     8881 tcgtcgtcga cgccgtacgc tcccgggagg tcggcgcgcc ccgggtcgtc gtggacgtcg
     8941 aggccgggag gagcgacggc ggcccccttg acgcgcgaga ggaccgagcc gcgctcgccg
     9001 ccgtccgcct cgagggagtg cccgaccacc tcgtgccaga agacggaggc ggccgagggc
     9061 gagaagacga cgtcgaccgg tcccggctgc ggcgggaggg cggggacggg gcggatcgcc
     9121 tcgcggaggg cgagggcgag ggcgtccgcg gcctcggcga acggccgcgc cgactggaag
     9181 gagaaggggc gttcggcgcc ggccctgcgg atcgtcccgg tcgccacgag gcgcgtcctc
     9241 gcgccgcagg gcaggaaggc ccgcggcgtc acgaccgtcc gctccgtcac gacccgcgag
     9301 agctggagag cggcggtgac ccctcgcgga tcggggaagg cgcgcgcgag ggccgcggcg
     9361 agcgcggcgg aacggctctc ctcctcctcc ggccgttcgg cgtccctccc gcccggcgcg
     9421 gcgccggtcg tccggtgggc cttgaagaag ggcgcgttcc cggcgagccg tcccgcctcg
     9481 cggacggcgt cccgcagcgc ctcggggccg gcgccggagc gcgcgacgag gaggaggcgc
     9541 ccctcgcgcc ggatccggac cgccgtgccg ctctccacgg agagggcggg agcgaggagg
     9601 cccgactcgg tggcggacca gctctcggtc ctcacgcgtt cccggaacgc ctcccccgtc
     9661 tccccgcggc ggaggagccc cgacacgagc ctcacggcgt cgtccgcgtc gcgtgcgagc
     9721 tccggcagct ccttcatcac cggcccgatt ctaggcagtc gcaggacgga gcgctcgggc
     9781 cgctgctgta tcctccgcgg ccatggccgc gcccgacgcc cgactcgccg cctgcgccgc
     9841 gttcgtcgca cggcgcctcc tcgactcggg cctcgtgaag gcgggctcgg gagcgcccgt
     9901 ccggaaggcc gtcgagtccg tcctcgtctt cgaccgcgat cgtgagcggg acctcgaggc
     9961 ggaggtcgag aagctccttc gcgcccacgg cgccgcgacg ctcggcgcgg gcgtcgacta
    10021 cgccgagatg ttccgcaagg cgaagcgcct cctcgccgag aagaagaaga tcccgctctg
    10081 agggacggag cgacgcgatg aggacgatcg aggagaggat ccgccacctc gacggactgc
    10141 gccgggcggc gcggctcggc gggggagagg cgcggctgaa gaagcagcac gaggccggca
    10201 agctctccgc gcgcgagcgc gtcgagctcc tcggcgaccc gggctcgttc gaggagacgg
    10261 acccgttcgt cgtccaccgc tgccgcgatt tcggcatgga cgccgagggg aacacggtcc
    10321 ccggggacgg cgtcgtcgcc ggcgcggtgc gcgtcgaggg gcgccccgtc ttcgtcttcg
    10381 cgcaggactt caccgtcttc ggcggctcgc tctccgagac gaacgcggcg aaggtctgca
    10441 aggtgatgga cctcgcgatg agggccggcg ctccggtcgt cggcctgaac gactcgggcg
    10501 gcgcgcggat ccaggagggg gtcgcctccc tcgcgggcta cgccgacatc ttcctgcgca
    10561 acacgctcgc ctcgggcgtc gtcccgcagc tctccgcgat catgggtccg tgcgccggcg
    10621 gcgccgtcta ctcgcccgcg atcaccgact tcgtcctgat ggtccgcggc accgcgtcga
    10681 tgttcgtcac cgggcccgac gtcatcaagt cggtcacgca cgaggaggtg acgaaggagg
    10741 cgctcggcgg ggccgagacg cacgcttccg tctcgggcgt cgcgcacgtc gtcgccgact
    10801 cggacgccga ctgcatcctg aagatccgga cgctcctcgg cttcctcccg tcgaacaacc
    10861 gccaggaccc gccccggcgg gagagcgccg acccgtgcga ccgagaggac ccggagctcg
    10921 acacgctcgt ccccgccagc ccaaaccagc cgtacgacat gaaggacgtg atccggcgcg
    10981 tcgtcgacga cggcgagctc ttcgagatcc acgccgactt cgcgaagaac ctcgtcgtcg
    11041 gcttcgcgcg gctcgggggg cgccccgtcg gcatcgtcgc gaaccagccc gacttcctcg
    11101 ccggcgtcct cgacatcgac tcctcgacga agggggcgcg cttcgtccgc ttctgcgacg
    11161 cgttcaacgt ccccctcgtc acgttcgagg acgtcccggg tttcctcccc ggaacccagc
    11221 aggagttcgg cgggatcatc cggcacggcg ccaagctcct ctacgccttc gccgaggcga
    11281 ccgtcccgaa ggtcaccgtc attacgcgga aggcctacgg cggcgcgtac tgcgtcatgt
    11341 cgtccaagca catccggacc gacttcaact acgcctggcc gacggccgag atcgccgtca
    11401 tggggcccga gggggcggtg aacgtcctct acggccgcga gctcgcgcgg agcgccgacc
    11461 ccgccgcagc gcgcaaggcg aaggtcgagg agtaccagga cctcttcgcg aacccctacg
    11521 tcgccgccca gaagggatac gtcgacgacg tcatcctccc ccgcacgacg cgccgacgcc
    11581 tcatccgcgc cctcgacctc gtcgagaaca agcgcgacgt cctccccccc aagaaacacg
    11641 gaaacatgcc cctgtgacgc cgcttccgca gaacgagggg tgggggtccg gggggcgcgc
    11701 gaagctcagc cccccggaga ggcatgccgc tgtgagggga gggggtctga ggggaggggg
    11761 tctggtctac actctacagt ctgcgcggct ccgaccgccc gtagagtgga attccggggg
    11821 gcctgacccc cactctacgg ggtgcgcgtg aggctgctga tcgcgaaccg gggcgagatc
    11881 gcggtgcgga ttgcacgggc ctgccgggac ctcgggatcg agcccgtcgc ggtcttctcg
    11941 gacgcggacc gcgccgcgct gcacgtccgg ctctccgacc acgcggtcgg gatcgggccg
    12001 gcgccggcgc gcgagtcgta cctcgttccg gagaagctga tcgacgcggc gaagcggacg
    12061 ggcgcgagac tcgtccaccc ggggttcggc ttcctcgccg agaacgccgc gttcgcccgg
    12121 gccgtccgcg acgcggggct cgtctgggtc ggccccgacc cggagtcgat cgcggcgatg
    12181 gggagcaaga cggagtcgcg cgcccggatg caggcggcgg gcgtcccgat cgtccccggc
    12241 atgacgtccc cggcgaagga ccccgccgag atcgcggcct tcggccgcaa gcacggcttc
    12301 ccgctcctgc tgaaggcggc ggcggggggc ggcggcaagg ggatgcgcgt cgtccggagc
    12361 gaggaggagg tccccgcggc gttcgagagg gcgcggagcg aggcgcgctc gttcttcgcc
    12421 gacgaggcgg tctactgcga gctctacgtc gagcggccgc gccacgtgga ggtccaggtc
    12481 ctcggcgacc ggacggggcg cgtcatcgcg ctcggcgagc gcgagtgctc gctccagcgg
    12541 cgccaccaga aggtgctcga ggagtgcccc tcgcccgtcg tgacgccgga gctccgcgcc
    12601 cggatggagg agtccgccgt cgccgcggcg cgctccgtcg actacgtctc ggccggcacg
    12661 gtcgagttcc tcctcgcctc cgacggccgc ttcttcttcc tcgagatgaa cacgcggatc
    12721 caggtcgagc acccggtgac cgagatggtc tacggcgtcg acctcgtcgc cgagatgatc
    12781 cggatcgccc tcggcgagcc gatgagcgtc gcggcgcggc ccgagccgcg agggcacgcc
    12841 gtcgaatgcc ggatctacgc ggaggacccc gcgcaaggct tcgcgccgtc gcccgggacg
    12901 atcgagcgcc tccgctggcc gcagggaccc ggaatccgcg tcgactcggg cgtcgaggag
    12961 ggggacacga tccccctcga ctacgacccg atggtcgcga aggtcgtgac gcacgggcgc
    13021 gaccgcgacg aggcgctccg ccggatgcgg agggccctcc gcgagacgcg cgtcgacggg
    13081 atcgagacgt cgattccgtt cttcctcgag ctcctcgagg acgccgacgt gctctcggcg
    13141 aacgtctcga cgcagttcct cgagtcgttc gcgttcgagc cctccgtccc ctccgccgac
    13201 gcggaggaga tcggcctcgt cgccgcggcg gtggcggcgg cgcgcgcgag cctcgcccgg
    13261 cgcggggcct cgcagcctcg gccgccctcg gcctggcgga ccgcccgccc gacctacgga
    13321 caccgcgaat gaagtaccgc gcgaagctga ccggaccggc cggggagagg accgtcgagg
    13381 tcgatgtcca gctcgacccg gccggtgtcg tcgtgactct cgacggccgg ccggcgcgct
    13441 gggacgtcgc cccgactccc ggcggagggg cctcgctcct gcgcgccgac ggccggcagg
    13501 ccgaggcggt gccgggccgg agcgccgagg gggcggtcct cgtccgggtc ggcgccgagc
    13561 gtgtggccct cgagctcctc gacgagctga cggcgcgcgc gcaggccgtg gccggcgccg
    13621 ggagcgcccg tggctcgggg gacgtgaagg cagcgatccc gggccgggtc ctccggatcc
    13681 tcgtcgcccc cggggacgag gtgacgcgcg gccagccggt cgtcgtcctg gaggcgatga
    13741 agatggagaa cgacgtgcgc gccccgcgcg acggcgtcgt cgcctccgtc gaggtcgccg
    13801 ccgggcagcc ggtcggcgcc ggacagctcc tcctccggct cgagccggcc gtctgagcgc
    13861 cgggtcccgg cacgcccttc gctatcatcc cggccgcatg ccggtcagac gtcttcggcc
    13921 cgtcggaacg gcgccccgcg cgctccggcc ggccgcccgc gaatccatcg tccagctcga
    13981 cagaggaggc tcgtccgtcc gatgaagcac taccgctccc tcgtcctatt cgccgccacg
    14041 gcgctgctcg cgtgccgggc cggcgggacg ccgcagtcgg ccgcgcccga gaagccggcc
    14101 gtcacgccgg ccgcgtcgcc ggccccggcg gcgtctccgg ccgcgccggc cccggcggcg
    14161 aaagtcgccg atccgagaac cgaagcgctc gttgcctggc tcggggagta cttcccctgg
    14221 ggcccggggg agctgaaggt cgagccgctc gacgccgtcc gcgtcccggg ctggcgtctc
    14281 taccgcgcga cgaagacctt cgagggcgac cagagggcga acgaccaggc gttcctcctc
    14341 gcggacgacg ccgggaagac ggtcctcgcc ggcgacatct tcgtcgagga ggaacggctg
    14401 cgggcgccga agccggtccg cgggccggag gacgtcggcg cgctccgcga gctgctcgcg
    14461 cggttcctgc gcgggcgctt cgacgtcgtc ttcgagccgt cgcgcgacct cccttcgttc
    14521 aagggcctca cgatcaagca cgagacgggc tacggcagct acgacatcgg cgcctacgtc
    14581 acgtccggcg acggcgccgt cctcctcgtc ggccgggcgt gggagagggc gaagagcatc
    14641 gccgcgcagc gccgcgcgct gatccgcgtc gacggggctc cgacgtcggg tccggccgac
    14701 gcgacggtga cgatcgtcga gtactcggac atgcagtgcc ccttctgcaa gaagcgcgcg
    14761 gccgacctcg acgcgctcct cgcgaagatg tcgaaggagc tgaaggtccg gcgggtctcg
    14821 aagtcgttcc cgatcgccga ccacgcctgg gccttccgcg cctcgtcggc gggggtctgc
    14881 ttcgccgact tcaagaaggg gctccacgcg tactggaagt ctgccgtgtt cgcccggcag
    14941 gagacgctct cggtcccgga gctcgacacg ttcgcgctcg acttcgccgt cgccaacggg
    15001 atgtccgagg aggccttccg ggcctgctac ctccaggggg gcgccgtcac gaaggtgctg
    15061 gacgacctcg ccgaggggtt cgccgtgcgc gttcggtcga ccccgaccta cttcgtcgac
    15121 ggggtgcctc tctcgtggtt cagcgacccg ctgatggagg actacctccg gaagacctac
    15181 ctgaagggcg ccgcttcacc ggccgcggcg aagtagcggg ccgggccgcc ggttcggcga
    15241 aaggagaagg gcgggacgcc ggccggcgtc ccgcccttct ccgctccttc gtgtccgagc
    15301 gctcgggccg ctaccggacg acgaacggga gaggcacgac gacccacgtc ttcacgggga
    15361 ccccgtcctt ctccgccggg gtgaactccc acttcttcac ggccgccagg ctggcctcgc
    15421 cgaggccgac cttcgggttc gtgtcgcggt agatctcgac ctcggccggc cgtcccttct
    15481 cgtcgacgag gacacggacg aggacggtgc cggagacctt cttctggcgc gccaggctcg
    15541 tcgcctcggg cttgacgacc ttgaccgcgc gaggctgttt cgtgacctgg ccgatctcga
    15601 ggaggtcgcc cgccttcacc ttcggcacgg cggcctcggc ggccttccgt gcgctctcct
    15661 cgcgggcgcg ggcctcctgg gcctccttcg cgagccgctc cgcctcggcg gtgtcggacc
    15721 tctcgcgggc cgcgcggacg gcctcttcgg cctctctcgc cttcgccgcc gcggcctcca
    15781 gggccgcctg ctcgtccgcg cgccgcttcg tcgccgctgc ctgctccttg gagatctggt
    15841 cctggacctt cttcatctct tcctggagct tcgcctggag cgccgcctgg aactccgggt
    15901 cctccttgcc gacgatgacg ggagtcggcg cgggcatcgg ggtcggcgtc gccacgggga
    15961 ccggggtcgg cgggggagcc gcgggctgct tcggaccgcg gccgaggagg agccacccgg
    16021 ccaggccgag gacgacgacg gcccctccgg cgacgacggg gacggggatg ccgccgagcc
    16081 cgcccttctt cgccacgggc gttccgccga gcgtcgtcgt gccgggagag gtgccgccgc
    16141 cgaacgacgg aacggggatc gaggccgcgg cgcgcgcggc ctgcgccttc agccgggcgt
    16201 ccttgtcctt cttctcctcc gccgcgacga gcggcttcgg gtcgatcgtc tcctcctgcc
    16261 cgagctcgat ccgctcgcgc tcgatcgcct tctcgaactt ctggtgcatg aagaaggcga
    16321 ggttgaacgt ggagggggcg taggggccgc cgtagagcag cttgccgagc gtcttccggt
    16381 aggccacgac gtcctgctcg cgctgggccg ggtccttcgc gaggccgcgg gccaccagct
    16441 tgccgaggtc gtccggcatc gggctcccgt cgatggcgag gacggcgccc gcgagggaat
    16501 cggcgcccgc gccgggggcc ggcggctttc cggtgagggc gtggaagagg atcgccgccg
    16561 ccgagtagat gtcgccggcc acggtcggct tgcccgacgc gaggacctcc ggagcgatgt
    16621 agggggcgaa cgcggaacgg atcggagcgg acttcagcga cggcaggagg ccggagccga
    16681 ggcccgcgcc gtagaccctc acatcgccgt cgttcgagat ggtgacgaag cccggcgtca
    16741 ggaagccgtg cggcgccccc atcttcttct cgaacggctt gcccgcctcg agggcgacga
    16801 gcagcttctc ggcgatcagg agcgcgtgct ccacggggag cgggatcgcc ccctgcccct
    16861 cgccggagaa gaggcgatcg agggtccggc ccggcacgta ctcgatgccg gcgaacggta
    16921 cgtcgtccac gatgccgagg accgcgccct tcgcgaccgc gggaccgcgg acgacctcga
    16981 ggtactcgga ggcctcttcc atcacgggga cgagggaggg ctggctgata ccggccccct
    17041 cgaagacgcg gatcaggagg aatggcgcga ggcgggcccc ggtggccgtc ccggcccggt
    17101 agaggccgcc gagcgggtcg gtgccgaggt gccgggtgag gacgaagggg ccgaaaattc
    17161 tggggaccgg gccgctgctg ctcatcttcg ctccgcttcc ggatgacgtt ccggagaacg
    17221 aaaagtctac ctcagcggga tgcgccgaag acggtctccc gcgcggtccg ggcgcgcctg
    17281 tagagcgcca cgttctcgag gtgctccggg tacgtcaccg cgaagcggtg ctcccccgag
    17341 ccgtcgccga cggcgacgaa gtagaggtag gggacgccgg cgggcgccac ggccgcttcc
    17401 agggcggcgg cgccggggct cgcgatcggg cccggaggga ggccgccgcg cgcgtaggtg
    17461 ttgtaggggt cgtcgacggc gagagaggcg cgcgtcagcc cccccgcaaa gagcccgcgc
    17521 cgcttcaggg cgtagacgac ggtggggtcg cactggagcg gcatgccgac ccgcaggcgg
    17581 ttgtggtaga cggccgagac gagcggccgc tcccgggcga cgcccgtctc cttctcgacg
    17641 agcgacgcga gcgtgacggc ctcgagcggc gtccggccga gggcggcggc ccgctcggcg
    17701 aagccctccg gaagcgagtg ggcccaggcc ttcacgaggg tggcgacgat ctcgcggggg
    17761 cgctgggacc gggtgaagag gtgtgtctcg gggccgagga acccctccag ggtcggggcg
    17821 tccggcggga cctcggggaa gagagccggc ttcaggaaga gctctcgaag cggctcggcc
    17881 ggggcgagcc cctcgcgctc gaggagggcg aagatctcct cggcggtcca tccctccgga
    17941 atcgtcaggc gatgggtgac gacgttcccc tcgacgaggt cgcggaggac ctcctcggtc
    18001 gagacggggc gcgtgaagcg gtactccccg gccttcaggg tcttcccctc gaagaggacc
    18061 cggtaggcgg cgccggcgag gtgccggttc cggatgacgc cggcgcgctc gaggcgggcg
    18121 aggatcgacc gcgccgaggc gcccgacggc agctcgacga cgacctcgcc gcccgtccag
    18181 ccctggtacg ggctcctcac gaggaagagc gcggccccgg cgccaagcgc cgcgacgagc
    18241 gcgaggaggc cgagcccgcg gaggccgatg cggaggagac gcctcaccct ctcgccccgt
    18301 ccgggtggga agagagccag tcccggagga gctcggcggc ggcctcggcg tcgaccgccc
    18361 cggagcgccg gtcccgggag aggccggcct cgcggagcgc tccctcggcc gagaccgagg
    18421 tgaaggcctc gtcgtgcagc tccacgggga ggccggtggc ggccgcgagc tttttcgcga
    18481 agctccgggc ccgcgcggcg aacgggctct cgccgccgcc ctcgggccgg acggggaggc
    18541 cgacgacgac gagcgcggcc tcccgctccc gcgccgcggc ggcgacggcc gaaaccgcct
    18601 gcgggtcgct cttccggtgc acgacgccga cggggacgac gacggccccc gccgggccgg
    18661 agacggcgag accgatcctt cgctccccga agtcgacggc caggattcgc acgaatcgag
    18721 gataggggtc cgcctccgcg ggtgtccatc cccggagaga ccgcccggga gcggatgagt
    18781 agaatccttc ggtcccccgg aggcccctgc gtggcccggg gggcctacgg agcaagcgaa
    18841 cgatgaagaa cgtcctcgtc ctctcggcga cccgaacgcc gatcggcacc ttcctggggt
    18901 ccctcgcccc cctcaccgcg tccgagatcg gagcccgggc gctcaagtcg tgcctcgagc
    18961 ggaccgaggt ccccggctac cgcgtcgacc aggtcctgat ggggaacgtc ctccaggccg
    19021 gccaggggca ggccccggcc cggcaggcgc tcatcgccgc cggcctcccg aagtcgaccg
    19081 gcgccgtgac gatccacaag gtctgcggct ccgggatgcg cgccgtcatg gacggcgcga
    19141 acgcgatccg ggccggggag tccgagatcg tcctggcggg cggcatggag tcgatgtcga
    19201 acgcccccta cctcctccag aaggggcgga cggggatgcg gatggggcac gggcagctga
    19261 tcgacgggat gatccacgac ggcctctggg acccctacaa gaacgtccac atgggcaact
    19321 gcgccgagat gtgcgcggcg aggtactcgt tcacgcgcga ggcgcaggac gccttcgcga
    19381 tggagagcta ccagcgggcg cgccgggcga acgactcggg cgacttccgc gccgagatgg
    19441 tcccggtctc catcgagggg aagaaggggc cggtcgtcgt cgaccgcgac gaggagccgt
    19501 tcgcgacccc gctcgagaag ctcgagaaga tggggtccct gaagcccgcc ttccagaagg
    19561 acgggaccgt cacggccgcg aactcctcga agatcaacga cggggcggcc gcgctgctgc
    19621 tggcgaccga ggcggtcgca caggagacgg gggcgaagcc gctcggccgg atcgtctcct
    19681 tcgcctccca cgcccacgag ccggagtggt tcacgacggc ccccgcgttc gccgcgaaga
    19741 aggcgctcga gaaggcgggg atcccgctct cgcagatcga cctgttcgag gtgaacgagg
    19801 ccttcgccgt cgtcacgatg gccttcgtga aggacctcga ggtcgacccg aaccgggtca
    19861 acgtcaacgg cggcgccgtg gccatggggc acccgatcgg ggcctccggc gcgcggatcc
    19921 tcgtcacgct cctccacgcg ctgaaggccc ggaagaagaa gtacggcctt gcggcgatct
    19981 gcatcggcgg gggcgaggcg acggcgatgg tggtggaggc cctgtgatcg acaaggtcgt
    20041 cctctcctcc tcgcatcacg aggcggcggc ccgcgcggtc gcggacatcc cgtcgggggc
    20101 gacgatcgcc gtcggcggct tcggcctctg cggcatcccc gagagcctga tccaggcgct
    20161 cgccgaccgc gacgtgaagg acctcgtctg cgtctccaac aactgcggtg tcgacgactg
    20221 gggcctcggg atcctcctcg gcaagaagat gatccggaag atggtctcct cctacgtcgg
    20281 cgagaacgcc gagttcgagc ggcagtacct cgcgggcgag ctggagctcg agttctgccc
    20341 gcaggggacg cttgccgagc ggctccgggc cggcggcgcc ggcatcgccg gcttctacac
    20401 gccgacgggc gtcggaacgg tcgtcgccga ggggaaggag acgaagctgt tcgacgggcg
    20461 cgagtacgtc ctcgagcgcg gcatcgtcac cgactacgcg ctcgtcgccg cctggaaggg
    20521 ggaccggcac gggaacctcg tctaccggaa gacggcgaag aacttcaacc cgatgtgcgc
    20581 cacggcggga aagatcacga tcgccgaggt cgagcagctc gtcgacgtcg gcgagatcga
    20641 cccggacgag gtcgacaccc ccggcgtctt cgtgaaccgc ctgatcgtcg ctccccgcgc
    20701 caagcggatc gagcggcgaa cggtgaggaa gggctgaggg ggcggcgatg gcgaagctcg
    20761 aaagggacgg aatcgtcgcc cgcgccgcgc aggagctgcg cgacgggatg tacgtgaacc
    20821 tcggcatcgg gatgccgacg ctcgtcgcga acgcggtgcc gcccggaatg gaggtcgtct
    20881 tccagtcgga gaacggcatt ctcgggatga gcggcttccc gaccgaggcc gaggtggacg
    20941 cggacctgat caacgccggg aaggagacgg tcaccgtcat ccccggcgcc tcgttcttct
    21001 cgtccgccga ctcgttcgcg atgatccgcg gcggccacat cgacctctgc atcctcggag
    21061 cgatgcaggt cgacgccgaa gggtcgctcg cgaactggat gatccccggc cgcctcgtga
    21121 aggggatggg cggcgcgatg gacctcgtcg ccggcgcccg ccgcgtcatc gtgacgatgg
    21181 agcacgtcgc gaagaagggc gacaagaaga tcctgaaccg atgcacgctc ccgttcaccg
    21241 ggaagcgctg cgtccaccgg atcgtcaccg acctcgccgt cctcgacgtg aagcccgacg
    21301 gggcgggcct cgtcctcgtc gaacgggcgc cgggggtgac cgtcgaggag atccaggcgg
    21361 cgacggagcc gcccctcgag gtcctcgggg acgttcccga gatgaagctc tgagccgggg
    21421 ctcccttcgg tcccggacgc cgcggcgggc ccccctcctg gggctcgccg cggcgctgct
    21481 cctcgtcgcg gcgcccgcgg cggggcagtc gtccttcgag agcgacccgt tcgcctcgcg
    21541 cttccgcgag cgcccgaacc tcgacatccg cttccgggtc ccggaggcgg gaggcgtcat
    21601 cacggtctcg gtctccgacg gtcagggggg ccggcagagc ctcctctcgg agacggtcta
    21661 cgaggtccag gccccgaagg ggggcgtcgt cacgctggag taccaggacg tccggctgac
    21721 ggccgactgg gtgcgggccg acacgaagac gaagcaggtc gtcgccgagg gggacgtcgt
    21781 cctcgagcag gggacgacgc gcctcaaggg gctgcgcctc gagatggacc tgaccgacaa
    21841 gaccgggatc ctgaccgacg ggagcgcgga gctcgcgggg atcctcgtcc gcggggcgac
    21901 gctcgcgaag accggggcgc ggacgttcga gatccgggac ggctcggtca cggcctgcga
    21961 aggggacgac ccggcgtggg agttccgcgt gaaggacgga gctgtcacgc tggacgacta
    22021 cgcgaagctg aagtcggtcg tcttccgcct cggcggcgtc ccgctcctct acacgcccta
    22081 tctcctctgg ccggcgctgc gcgaccgggc gtcgggcttc ctcgtgccgg gaatcggcta
    22141 cagcacgagc cggggcggct tcctcggcct ctcctacttc caggtgctcg ggcgttcggc
    22201 cgacgcgacg atcacgaccg acctctacac gaaggagtac ttcggcctgg ggaccgagct
    22261 ccgcctgagg ccgacggccg ggaccgcgtt caccggctcc ttctacaccg tcgtcgaccc
    22321 cgaccggcgc tggcagtgga agacgtgggg gacgctctcg gcagacgacc tcgcgccccg
    22381 gacgcgcgcc gtcgtcaact ggaccgactt ctccgacctc gcgttcttcc agcgctacga
    22441 ccgcgacttc gcgctcacct cggcccggac cgtaggccag aaggcgttcg tcacccgctc
    22501 ggaggggccg gtcgcgttca acctccggct cgagcgcgag acggcgctct acggctcgag
    22561 cgaggtcgtc ctggagagaa agccggtcct cgaggcgcgg ctccggccca cgggcctctt
    22621 cgggaacgcg ctcttcgtcg aggcgcaggg ctcggcggga ttccttcgct cgatccggcc
    22681 cgccgggcag ccggcgggag actacgagcg cttcgacctc tacccgaagc tttcggcgcc
    22741 cctctcaccg ctcccgtggc tctcgctgaa cgcggaggcc ggctaccggg tcacgcgata
    22801 cggcgcctcc gtctccgccg acgggcgatc gctcaccgag gagccctact cgcgcggctc
    22861 cgcgaccgcc ggcctcgagg tgacgggccc gtcgttctcc cgcgtcttcg acgtgaagct
    22921 cgggaagttc cgcaagctca agcacgtcat cgagccgcgg gccgactact cctaccagac
    22981 cgacccggag gacctctccc ggactcccgt cttcgacgag gtcgacacga tcctcccgtc
    23041 gcaccaggtt cgttacgcgc tcgtccagcg gctcctggcg aaggaggaga agggcgcctc
    23101 ccgcgagatc gcctcgttcg agctctcgcg cacgcactac ttcgagctcc ccgtcggcgc
    23161 ggccacgacc gttcgcgact ctccgctcga cgcggtcctc cgcgtcaacg cgggctccgc
    23221 cctcaacttc gacgcgcggg cgacgtggga cctgaaggaa ggcgtcgtca cctcctcgag
    23281 cgtgtcggcg aacgtcggct cggccgaacg cttcctccgg ctctccttct tcgacagccg
    23341 ccccgcagga gcgccgacgc cctccaccca gctccgcttc ggcgggggct cctccctcct
    23401 gccgaagcgg ctccgcctcg acgtcgaggc gaactacgac gtggagcgcg gaaagatgct
    23461 cgagtggcgg agcctcctga cctgggaggc gtcgtgcttc aagatcctcg gcgagtaccg
    23521 cgacctgcgg atcggcgcga tcccgacgcg cgactaccgg atcgggctca acctgaagaa
    23581 cgtcggaagc ttcctcgact tcaccgggcg cgtcgggaac tgacccggac cgtgccgtcg
    23641 gctccccgga ggtgtgcgga atgaacgagc tgatcctcgg cgccggcgga atgctcgccc
    23701 gcggactcgc cgcgctgcgg ccccgcgcca gagcgctggg catcgccgag ctggacgtca
    23761 ccgacgcggc cgcgctcgcg cgcgtgatcg tcccgggcgt ctcggtcgtc tggaacgcgg
    23821 cggccgacac gcgggtcgac ctctgcgaga gcgaccccgg ccacctcgcc gtgaacgacg
    23881 tcgccgtcgg cgaggtcgcg cgcctctgcc gcgacgcggg ggcgctcctc gtccacgtct
    23941 cgaccgacta cgtcttcgac ggcaggcccg gacggccctg gcgcgaggac gaccccgtcg
    24001 acccggtcaa cgcctacggg cgcggcaagc tcggcggcga acgccgggcg ctggcgagcg
    24061 gggcggaggt cctcgtcgtc cgcacgtcct gggtgttcgc gccgggcggc gtcaacttcg
    24121 tcgacacgat cctcggcctc gcggagtcgg gccgtgagga gctgaaggtc gtcgacgacc
    24181 agcggggccg cccgacgtac gcgccggacc tcgcccgcgc gctcgtgaag ctcgtcggct
    24241 ccggcgcgcg cggcctcgtc cacttcgcga acgccgggga gacgacctgg tacggcctcg
    24301 cgaaggaggc gctcgcccgg gccggcttcg ccggcgtgag agttctcccg tgcgcgagcg
    24361 acgagttccc gcggccggcc gcgcgcccgg cgaactccgt cctcgacacc tcccgctacg
    24421 aggagatgac gggcgaggcg ccccgccatc acctcgaggc gctcgacgag cacctccggg
    24481 tccgcgcgga gaccgggcgg gcgtgagcct cgacgccacg gaggcgagga ggaaggccgt
    24541 ccacgtcggg atggcctcgt tcgcgctcct cctccgttgg ctcgacgtcc ggggcgctgc
    24601 cctttgcgcg ctcggcgcgt tcgccttcaa cctcctcgtc ctcccgcgcc tcggcggccg
    24661 gagcctcttc cgtgaaggcg accgcgcccg cggctacccc gtcggcatcc tcctctaccc
    24721 gctcgtcgtc ctcgcgctcg tcctcctctt cccgcgccgc ctcgacctcg cggcggccgg
    24781 gtggggcttc ctcgcgttcg gcgacggctt cgcgacgctc gtcggcgcgt cgctcggggg
    24841 gcggcggctc ccctggaacc cgcggaagag ctggagcggc ctcctcggct acgtcgtctt
    24901 cgggagcctc ggcgcagcgg cgctctcgtc cttcgtcgcc ggacgcctcc cggcaccgct
    24961 cgagcacgcc gccttcctcc tcgccgcgct cgtcggcgcc gtcctcgaga gccttccgtc
    25021 ggagctcgac gacaacgtcg tccctccgct ggcggcggcg ggcgcgctcg cgctgctcct
    25081 tcccgccctg ccgaacgccg cgctcctcct ggagccggag gccctccggc gcctcgccgc
    25141 cggcgtcggg gtgaacgtcg ccctgccggc gctggcggcc gcgctcggcg tcgtccggcc
    25201 ctctggggcc gtggcgggcg gaatcgtcgg cgccctcgtt ctcggcctcg gcggcttcgg
    25261 accgtacgcg ctcctctggg ccttcttcgc cggcgggacg ctcgcgaccc gcttcggacg
    25321 ggcgcggaag gaggcgatgg ggaaggccga ggcggcgggg ggacggcgga gcgcggcgaa
    25381 cgtcctcgca aattgctccg tcgcggcgtt cctcgtcgcc tgcgcggcgc tgacgccggc
    25441 cgcctctggg ctcctgcatc tcgcggcggc cgcggcgttc gccacggcgc tgatggacac
    25501 gatcggcacc gaggtcgggc aggcgatccg gacgccgacc gtcctcctcc ccgaccttcg
    25561 ccgcgtcccg ccggggaccg acggcgccgt ctcgatcgcg gggacgctcg cgggcctcct
    25621 cgcggcggcg ctcctcgccg cgctcggcgc cctcctcgat ctctacgctc tcgccgctct
    25681 ccccgccgtt gtcgccgcgg ccttcgcggg aaccgtcgtc gagagcctgc tggggcgcga
    25741 cggcgcctcc tggcgcgtga cgaacggcca cgtcctgaac ctctacaaca cgctcgcggg
    25801 cgccgcggtc gcggcgctga tcgcgcgctt cgctctctga gcgctccggg agagagttcc
    25861 gtcgacgccc cggcgcgccc cggggcggcg ctgcccggag tcggggatcg cctgcccggg
    25921 ggagcgttcc ggagtcgtct tcggtgaatg gactctacgg atcgtccgcg gtctccgcga
    25981 tgacggtcgt cacgcggaag gcgagcggac gggagcgtcc ctcaggctcg aaaggcgtcg
    26041 acgacgcgcc ggaggcgcgc gccgatgtcg gcgccgacct cggcgagctc ctccgagccg
    26101 aggatcccca tgacgccggc cgggtccatc gcgcggacga cggtcgtccc gtcctccacg
    26161 gagaccgtga cgttgcaggg gaggaggacg ccgacctccg gaacggccga gagggccttg
    26221 tgcgccgacg gcggatggca ggcgccgagg atgaggtagg gctcgcggtc gacgtcgagc
    26281 ttcttcttca gcgtcgccgc cacgtcgatc tcggtcagga cgccgaagcc ttccttcgcg
    26341 agctcctcgc gcgcgcgggc gacggcgtcg gcgaaggaga gggacgtggt cgtctgcagg
    26401 gcgacggggg aatccttcat cgaagctcct tacgcgcccg cgggcgccgg atcggtgagc
    26461 ggaggggcgg tgcggcgggg gcccgacggg ccccccgggc ttaccctcac gtgaagcggg
    26521 tgccgcagcc ggaacccccc ttcggcgagc cggacgagct cgcgccggag gcgaagctcg
    26581 agagcttgcg ggaggtgcgg cgtgactcgc aggacgggca gtcgatcgcg gcgtcggcct
    26641 cgctcgaggg gcggagctcc tcgaaggtcc gcccgcattc gtcgcatgag tactcgtaga
    26701 ggggcatcgt ctcgcctcgg gggaggcgcc gcgtgtcgcg gccctcccgt gtcgtcgtgc
    26761 gccgagccgg ccggctcagc agcctccggt cagcttctcc cagacgtggg ccgggaggcc
    26821 ggaggggtag atctgatcgg ttcggtagag gttccagcag ccctggcacc gccagagcag
    26881 gaccccccct cacgtcggga cggggaggac ctcctcgccg cacacgcggc agcggccttc
    26941 ctcgacgctc acgggggagc ccgcggtctc ggcgttcatc gttctcctcg cgcgtccgcc
    27001 ttcggggcgg gcgccgcttc cgggcgctcc ccggacaccg ggttcgtttc gttcgtcgtc
    27061 gcaggcttcg tcgcgcagct ccggccgccg gccggattcg ggaccgctca cgtcgggacg
    27121 ccgagcctcg ggaggacgac gtactgcagg agcacccaga cgacgaagaa gccgccgacg
    27181 agcaggaggt cggagctcat gcccgcgccc cggcggcgag cgccgccttc ccggccttca
    27241 gctcgacccg cctctcgtcc aggctcctct gcatcgcgcc acagacgacg ccacagatcg
    27301 cgaagaccgc ggggtccgcg atccggtagt agacctgcgt cccctgcctc cggaactcga
    27361 cgatccccgc cgtccggagg agggccaggt gcttcgagac gttcgcctgc gtgcacggca
    27421 gctccgcgag gaggtcgctc acgcaccgct ccccgccctg gagggcgtgg aggatccgga
    27481 gccgcgtggg gtcggcgagc accttcagga agctcgcgat cccctccatg agctgctcct
    27541 tcgggagcga ggcggccttc ctcatgacca tatcaccata cggtaatata acatccgccg
    27601 gcgggatcgt caagccgcct ccggcgggcc gggcgccgaa gcgcccggcc ccgccccggc
    27661 cgtgcgctct cgcccggccg gcggggctca gaggagctcc tccaccgccc aggaggcgat
    27721 cctgaggcgc tcgccgcact cggcgcagta cgccttgatc tcgtcggcga gcgcgcgggc
    27781 gcgctcgtcc tccacgatcg tgaagaggac ggcggaggtc ttcgggaacg cggccgagcc
    27841 gaggcggggg ccggtcgtcc ccatgccgga ggctccggcg atctcggtcc agccggtgac
    27901 gccggacttc cggaggagga cctcgagctc ctccttcttc ccgtcgtcga cgacgatcat
    27961 cagcatccgc acgcgccgcc ctccttcacg cccagcttcc ggagcatcgc gatcgccggg
    28021 caccacttcg tgaaggccga ctggaggagg ttcaggccga cgaagccggt gaaccagagg
    28081 aagcgctggt cgaccgtcac cgccaggacg acggagagga ggacgaagaa gccggcgatc
    28141 gcccggagcc attcgttgag gttcatgtcg gtgtctctcc ttcggttcgt attcgttcgg
    28201 gcgggacgcc cggtcaggcg gcctcgtcct gaggcggaag cgccttctcg acgccgaccg
    28261 ccttcaggta catgtagtag aggagcggga tgacgacgag ggtcagggcg gtcgcgacga
    28321 cgacgcccgc gatgagcgcc acggcgagcc cctggaagat cgggtcgagg aggatgacgc
    28381 cgccgccgac gacgagggcc gcggccgtga gcgcgatcgg gcggaagcgg acggcgcccg
    28441 ccttgatcac ggcgtcctcg agcttctcgc ccatctcgag ctcgaggttg atgaagtcga
    28501 cgaggaggat cgagttcctc acgatgattc cggcgagcgc gatgaagccg atcatcgacg
    28561 tcgccgtgaa gaagacgccg aagaggccgt gcgccggcag gatgccgatg agcgtgagcg
    28621 ggatcggcgc catgatgatg agcggcgtca cgaagctccg gaaccagccg acgacgagga
    28681 agtagatcag gacgaggacg gccgcgaagg cgatccccat gtcgcggaag acctcgtacg
    28741 tgatgtgcca ctcgccgtcc cacttcatcg cgtacttcgt ggagtcggtc gggagcgtcg
    28801 tcgacatcac ctggagcggg gagccgtcgg gcgccttcag cgacccgatc ctctccccca
    28861 tgtcgaggat cccgtagacg ggggcctcgg cgacgcccgc catctcggcg aggacgtagg
    28921 tcacgggctg gaggttcttg tggtagatga accgctcgga cttcgtctcc ttcacggtga
    28981 cgagctcgcg gagcgggacg aggcgccccg tgccgccgtg gacggcggtc tggaggaggc
    29041 cgtcgaggct cgagcgctgg gccctctcga ggcggaggac gatcgggacc gggttccggg
    29101 agctctcgtc gcggaggagc ccggcgtcgg cgccgttcag cgcgacgcgg agggtgcgcg
    29161 tgacgacctc gggggtgacg ccggcgcggg tcgccttctc gcggtcgacg acgagctcca
    29221 ccttcggggc ctcgtcctcg acgtgccagt cgacgtcgac gatgccgggc gtctcttcga
    29281 agatcgtccg gacctgccgg gcgaggtcga tccgcttcgc catgtcgggg ccgtagatct
    29341 cggcgaccat cgtggagagg acgggcggcc cggggggcac ctcggtcacc ttgaggttcg
    29401 cgccgtggcg cttcgcgatc ggaaggagca gcgtcctcac gtccttggcg aacgtgtgcg
    29461 agtcgcgctt ccgctcctgc ttgggcagga ggttgacctg gaggtcggcg acgagggggc
    29521 ctgagcggag gaagtaatgc cgcacgaggc cgttgaagtt gaacggcgcg gtcgtcccga
    29581 cgtagacctg cacgtcggtc acctcgggct gcctcctcac ctcgagcgcc aggtcccgcg
    29641 cgacggcgtt cgtcgcctcg agcgtcgtcc cctcgggcat gtcgacgacg acctggatct
    29701 cgctcttgtt gtcgtacggg agcatcttga ccttggcgac cttgaagtag accagcgagg
    29761 tcgagaggag gaggaggacg acgacgccgc cgatgagagc ccagctcttc gcggggctcg
    29821 tcaggagagg ctcgaagagc ctctggtaga agcggtggag cttgtcctcc tggggcgtct
    29881 cggcgtggct ctcgaacgcg gagcggttcg cgtgctcctc ggcgtacttc cggaagagct
    29941 tgaacgtgag ccagggggag acgacgaacg cgacgaagag ggagaagatc atcgccgccg
    30001 aggcgttgat cgggatcggg gccatgtacg gtcccatgag gccggagacg aagacgagcg
    30061 gcaggagcgc gccgacgacg gcgaacgtcg cgaggatcgt cggattcccg acctcgtcga
    30121 cggcgtagac ggtcgtcagg cgcggcgggc cccacccgag ctcgtagtgc cggtggatgt
    30181 tctcgacgac gacgatcgcg tcgtcgacga ggatgccgat cgcgaagatg agcgcgaaga
    30241 gcgtgacgcg gttcagcgtg tagccgaaga ggtaggacga cgccagggtg agggcgagcg
    30301 tcatcgggac ggcgacgagg acgacgacgg cctcgcgccg cccgagggcg aacgccatca
    30361 ggaggacgac cgagaacgtc gcgagcgcca tgtggaggat gagctcgttc gacttctcgt
    30421 cggcggtgtg gccgtagtcg cgcgtcttcg tcagcgtgac gttcgagggg acgaccttcc
    30481 ccttcaggcc ggccaccttc gcgtcgagct cctcgacgag ctcgaccgcg ttcgtccccg
    30541 gcttcttggc gacggagacg gtgacggccg ccgtgtcggt gccgggggcg aggccgcggt
    30601 gcgcggcgcc cgggcccgac agcatccaga cgtattggga gggctcgtcg gccccgtcgg
    30661 tgacggtggc gacgtcgcgc aggaagacgg ggcggccgtt ccagacggcg acgaccgcgt
    30721 tgccgacctc ctcggccgag cggaagaggg agccgacctc gacctgcgtc tcggagtccg
    30781 aggtagcgaa gctccctgcg gggaggcgcc agttcgcgcc ggagagcgcc atgtaggcct
    30841 gcaggagcga gacctggtgg gccgcgaggc ggtcgcggtc gaaggcgacc ttcacggccc
    30901 gcttctcgcc gccgaggacg gtcacctggg cgacgcgcgg gtgcttcgtc agctcgacct
    30961 tcagctcctc ggcgacctcc ttgagctgca tgggcgaggc gtccgcggag ctgagcgtgt
    31021 aagcgagtac cgggacgtcg tcgatcccgc gggggacgac gagcggcggc atcgcgccgg
    31081 gcgggagctc ggaggccttc tcggcgagct tcgtgtggac ccgcaccgcc gcctggtcgg
    31141 ggtcggtccc gacgaggaag cggacgatga tcatcccgcc cgagggctgc gtgatcgagt
    31201 agacgtactc gacgttcggg atctcgtaga tcgccttctc gaggggcgac gtcagccggc
    31261 gctcgacctc ccgggcggtc gcgcccggca tcccgaggaa gacgtcgatc atcgggacct
    31321 tgatctgcgg ctcctcctcg cgcggcgtca ccatcacggc gaacgcgccg acgaggaggg
    31381 aggcgacgac gaggagcggc gtcagcttcg actcgaggaa cgccttcgcg atccggccgg
    31441 aggcgccgac ggggcggcgg gtcatcttga ggcggagcgc ctcgcgcttc gtgtcggcgt
    31501 gggacggcgg cggggtcggc cccgcggcgc cctcggcggc cttgccggcc gcctcgtcgc
    31561 ggggctcgct cacgggacca cctcggagga ggcgttcacc ggggcgccgt cggcgggggc
    31621 ctgcgcgagg ccgacgagga cgtcctcgcc ccccttcagc ccggagagga cctcgacgag
    31681 gtcgccgtcg cgggtgccga gcgtgacggc gcgggtccgc gccttcccct cctcgtcctt
    31741 cacgacgacg aggctcatcg ccccctgacg gaggacggcg gcggcgggga cgacgacggc
    31801 cgtccggcgg cccgtctcga ggagcgcccg gccggtgacg ccggtcggca cctgggcccc
    31861 cgagacctcg acgcgcgccg tgaaggagtg gctcgcgggg tcggccccgg cggacatctc
    31921 gacgacgcgg ccctgcagcg tcgcgcccgg catcgcgtcg agcgagacgg ggaggagcgc
    31981 gccggtcttc agcttcgagg cgacggcgac gctctcgggg accgagagga cgagtcggcg
    32041 gcccgcggtc gactcgacga cgacgagcgg ccggccgggg gcggcgaggt cgcccgggtc
    32101 gaccatcttc cgggcgaccc gcccggcgaa cggcgcgacg acgcgcgact cgcgcgcgac
    32161 ggaggaggcg gcctcgacgg cgccggtcgc ctgctcgacg gcccccttcg cctgctcgta
    32221 ctgcatccgg gccatgtcga cctcgagctc ggaggccgcg gccgacttct ggagcgcctg
    32281 gaacctctgc cagttccgct ccgcgagggc gagggccgcc ttcgcctgcg cgagcgcgcc
    32341 gcgggcctgc gactcctgcc ccttcgccgt ctgcgggtcg atctcgacga ggagctggcc
    32401 ctgggccacg aggtcgcctt ccttcaccgg gacggcgacg acggacgcca tgacgcggct
    32461 cgagaccatg gcggacttgc ccgcctcgac ggtgccggag agctcgatgc gctgggggag
    32521 ctcgcgcgac tcggccttcg cgagggaggc ggagacgggg tcgggggtgg cgacgtcgcc
    32581 ggccttgtgc ccgcctccgc aggcggtgag cgcgagcgcg ccggccacgg tgagggtgac
    32641 gagggaaacg gtcttccggg aagtcatgcg agcctccgtg ctcttctgct gtgtcgggtc
    32701 ggggagaagg gaatggtgtg cgctcacggg gccaccgtct ccggggcctt tccggccttc
    32761 accgcgaggc ggagcgccgt ggcgtgggcg tccgcgcggg cgacgagctc gcgcgtctcg
    32821 gcctcgcggc gggcggtcgc cgcgtcgagg aggtcgatcg tcttcacgac gccgctccgg
    32881 aagc
//
LOCUS       DAOTEZ010000004        30734 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_980_length_30734_cov_6.511881, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000004 DAOTEZ010000000
VERSION     DAOTEZ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 30734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30734
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_980_length_30734_cov_6.511881"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="P5164_00410"
     CDS             <1..470
                     /locus_tag="P5164_00410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRY42378.1"
                     /translation="CFLKVWENLDRYEPTEASLLAWIATLSRNLSIDHYRKRRREKGF
                     HFVTDDALVSLLPGSDDPSADAVRRERLRLLLDAIGQLPDELAEVVQMRDLDGLDYRE
                     IADLLGLPDGTVKSRLNRARMELARIVRERIGAAPLVRGPLFGGLTPQTGGAA"
     gene            467..1270
                     /locus_tag="P5164_00415"
     CDS             467..1270
                     /locus_tag="P5164_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42379.1"
                     /translation="MTAGASAPCRRAEAALLAGGVPVPAEIGDHAAACETCGPLLSGC
                     EEIDALFASLAVPEPPPALSGALEAIGSVEPARVAVRETLALLAPGALAVPEPSPALL
                     RRLLAVAATGPRPEANVVEMSRFRRLRSDWRVLVAVAYAACLALVAILGVDPLSAARS
                     GASGMAAAGERAIAEAREVAADKLDTVLAAQRQKPLTEQLDYKVYRTLAIGKAKTSAW
                     AGILLGRIFGTRVDAEAPAAPRRSEPDPGSLRSFDRAGTDTPLSRAQRA"
     gene            1294..1881
                     /locus_tag="P5164_00420"
     CDS             1294..1881
                     /locus_tag="P5164_00420"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HRY42380.1"
                     /translation="MNENPNLPPPPANVTAAPPVAYVPPAPPPRPVAPPGKNPAAAGL
                     LSLFPGLGHVYLGLYKRGVVFFAIWAFFIAAVDRGHDMPFGLLIPFWMVFVLIDAVRQ
                     ARALNATGMPEPGFFGEEKPFRADGGLTFGIFLILIGLFFLADRIFTIDLSFLLDWWP
                     VLLVAFGGWQVFAYFQEKKRRDEVAAGREAGAVEP"
     gene            complement(2090..2620)
                     /locus_tag="P5164_00425"
     CDS             complement(2090..2620)
                     /locus_tag="P5164_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42381.1"
                     /translation="MAPLLALLTLFWLADPAPVKGVPPPPGGRFEPEMTRVVSDRAKA
                     EMNSVLAELERSVQPPEAKAEIRRRAAGLRLAVYTTPRGWDETVAFYEGAPMQLIFLK
                     GERDILGDLREWARAAGLAVDASAEKAWAGKSGRTARWTKDDETLQVVVEDHLIDPRD
                     GKVSETTVVLVTSLGG"
     gene            complement(2632..3564)
                     /locus_tag="P5164_00430"
     CDS             complement(2632..3564)
                     /locus_tag="P5164_00430"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRY42382.1"
                     /translation="MTSGRDLFAPKMGLTMPLCGLHFVFFFLFTMDPQKTAALFGFWP
                     QQALSQPWGFVTFQFLHGGPMSLFFGAFALYILGSALEAEWGTGEYTAFWLVATLGAS
                     LSAWVLGTPLVSDPFVVPISMLFAYAFLFPETQFLVFFVIPVKVKWIAVFSAALLGLA
                     FFRDLVTFGAGPAFVRILGAGAGFLFFWVRHHGRFKARKAALETVAAVKTTAVQQKDE
                     ALEKRNRELFPRVEALRQTERSGVEPPGGEGLRRELQKLVVPGVKICKPVDFKGDKDL
                     VCLRCEGFAECSLRYLAGQPAEIVVKKRDGGAAG"
     gene            complement(3561..5402)
                     /gene="mutL"
                     /locus_tag="P5164_00435"
     CDS             complement(3561..5402)
                     /gene="mutL"
                     /locus_tag="P5164_00435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRY42383.1"
                     /translation="MARIAVLPDVLVDQIAAGEVVERPASVVKELVENALDAGARRVD
                     VEIEGGGIARISVSDDGCGMDADDARLALERHATSKVRTAEDLGRIGSLGFRGEALPS
                     IASVSRLVLATSPDGSGLGTEVVADRGEAPVARPVRQTKGTRVVVEDLFGNVPARRKY
                     LKSQDAEQRAVVKALTSLALSRPDVAFTLRAGERELLALPAASAALDRFAEVMGRGTL
                     PRVIAVDFAHAGMRLLGAVSPPETTFASRTFQWLFVNGRAARDGSVSHAARLAAEEAL
                     LGERHPAWALFVSCPPERVDPNVHPQKSEVRFLDPGSVHALVHRGLRAALAEGKSATE
                     LPGSALAGTGDRPDDGIPIRAGGWGDAPPAAAALAETLGLWDGASGLAGASGFAAPGV
                     VPAPSRGSWGGAPAAAAVPSPAGTLRLLGQYRDSYLVAEGEGGLVLVDQHVAHERVRF
                     ERIRDRLEGQGLASQALLAPVSFEAAPEEAEALRRADGVLVAAGFSVSELAGRMFVVS
                     AAPADVAPEKVVAVLRDLLGRLLEASGTGAGRAGDTGARMKDALAASLACRGAITVNR
                     RLAPEEAARLLADLSACRDPWTCPHGRPILLTLAHAEVLKRFGRSSG"
     gene            5448..6203
                     /locus_tag="P5164_00440"
     CDS             5448..6203
                     /locus_tag="P5164_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42384.1"
                     /translation="MERSRRLSPALFVLAALVLAILVAASVLVGRVARLPREMAGAVP
                     HGIRETAAAVSDGLARLAAAIRTRSVTTELRSAVAELRGTKKLQVAELTQLEVVERRD
                     ATRLLGVPLPDVVVSARAPVTYVYTLDLDARWDFDLAERTVSVLAPDLVANAPAVDVS
                     KLELVKTESSILRDEDRVVEELRRSLTEVFRARARQHVPLVRETARKETEAFVRSWLV
                     RAYGLPEDVRVVVRFHGESATVGPVGVALPPRG"
     gene            complement(6218..7366)
                     /locus_tag="P5164_00445"
     CDS             complement(6218..7366)
                     /locus_tag="P5164_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42385.1"
                     /translation="MAPRPTGRDRLEPTTDGTVVLLSLRPKGWSGREEGGPRAPVRPG
                     TAVRWDGDLWEVLRVEPRAGGGWRHVLAPWDERNLVRSVLEYGEAGGTAESEAALPAA
                     DPPRDSGGAGGIAASSSPGPAAPGVAQPPPAPPPPRPRATGAEGVAAAWAALAPGVRA
                     LLVAFVPSVLLGWFFPFRVMGEGISFFVHELGHTFVAWFFGCTALPAIVMTVVFEQNR
                     GVAALVWGGLVFLAVRYRRAPRWNLVFAAVALLYPLFAFTKAHVTAFDLGGHLAEAAF
                     AAWAFQRVVRSERPEWERVVWAFFAFYVVARNVKLFGGVAFSAAARTDYLTVAITGQN
                     DLVKVAATTGVALTTLAFLVALLFLAVPLGALALSFRRPAAEASVVAT"
     gene            complement(7405..8037)
                     /locus_tag="P5164_00450"
     CDS             complement(7405..8037)
                     /locus_tag="P5164_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42386.1"
                     /translation="MTSMRTIALSVLAGACLVAACGKPAEKPAAPEAAPVAEAPAPEA
                     PAPAAPAGVTKVTVTSTNGAWPKENAPDFASPRAIAFKDVSVGVASVTVVLTNYDFAP
                     TTMAEYRKRTSPEPGQGRVELGLVRQKQVAPGETLEFVAGMYDPTKSQGQAEMTASVS
                     IAVTGGTSLSLSQPTGTIEVTSLTADSISGKFDVKDKWTQLSGEFHAPLK"
     gene            complement(8190..9638)
                     /locus_tag="P5164_00455"
     CDS             complement(8190..9638)
                     /locus_tag="P5164_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HRY42387.1"
                     /translation="MTHPLSGLEPKLLWDHFAALAAIPRGSKNEAAASAHVVAVAARL
                     GLPAKVDQVGNVVITKPGTKGLESKPVVVLQGHVDMVCEKNSGTAHDFTKDPIRLVVD
                     GDVVKADGTTLGADNGIGCAAALAILESNDLAHPPLECLFTIDEETGMTGANVLAPGF
                     VTGRRMLNLDTEEEGAVYVGCAGGVDTLATTKVERTKPTAALSPYRISVTGLRGGHSG
                     CDIHEGRVNALKVLARAVTEAGAVGFAAATLDGGSKRNAIAREGFAVGWLDPVKEPEV
                     RAALAKLEADVRSEAGRFDNPVNVGLERIDAAPDPIAEADLARIVGFIHAAPHGVLGW
                     SPDIPNLVQTSTNLAIVETKDDVVSFSTSQRSSVESAKKDAGRMMATAMGLAGFEVKQ
                     GDGYPGWKPNVTSPLLGLAKEVHAELFGKEPEVKAIHAGLECGIIGEKYPGMDTISFG
                     PTIRNAHSPDECVSIPSVKNFWTYLTAFLPRL"
     gene            9817..11844
                     /locus_tag="P5164_00460"
     CDS             9817..11844
                     /locus_tag="P5164_00460"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRY42388.1"
                     /translation="MRRPALLALTLLAALPLAAEPAAKRSLTVDDIWSVVRVGAPVVS
                     RDGTVAVYPRSVWDAEKNRLLSDLWLQPVAGGPGRRLTAHEAGESSPAISPDGSRVAF
                     VAKRDGDEVAQLYVLRLDGGEAERLTDLPAAPGSPRFTADGKRIVFSANVLGESLETT
                     KAELARRKKEEKTRVLATVSERRLVRYWDRWVEQDEWPHLLSVDVATRVVADLTPGLK
                     RLGRLEGGLEFDVAPDGSVVFAANATEPPFRTLNTDLFLVPAGGGTPRNLTADNPAED
                     SSPVFSPDGKTIAYGREAKGDGWPDRTRLALLDVASGKTKVLTESWDRVPQGWSFTPD
                     GRTLVFLAEDRARTHVWSLPVSGGTPKVVWKGGRANALEVAGPAGLVFTYTTFRRPAE
                     LATAKLDGSGFRNLTSWNDALMEQIAFGEEREVVFKGAGGDDVQMFVVHPPGFEKGKT
                     YPLVHVVHGGPIGTSGDEFHLRWNAHAFAAPGYVVALVNFHGSSSFGQAFVESILGAP
                     GDKPFQDVMAATDFLVAEGSVDPKRVAAAGGSYGGYLVNWIAGQTDRFAALVSHAGIY
                     SLLGQFGSDLAYGRQHSYGAYPFTNPSAIERWSPNRYAASLKTPMLVVHGEKDFRVPI
                     TQGLELYGVLTAKGVPARLVAYPDENHWVLKGTNAKHWYGEVLGWLARWLK"
     gene            complement(11906..13015)
                     /locus_tag="P5164_00465"
     CDS             complement(11906..13015)
                     /locus_tag="P5164_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42389.1"
                     /translation="MILPVLVAAALAGAPAVEAAPAPVPVEMDTQDRLDVPIALQLKD
                     ASVVDVLEKLADLLGVTPILDPGVGGRVSVEISNVPLRKALEMIGQAANVDVTVSSKV
                     LRARAKGASKATGAATAPAARTSDPVPLGGVLRFWLDGAGAPPVAVRVPDFAGRVEVP
                     GCAEPLTLGRLGARGGATDGIALAAVDPSGGGAKGRILGEAAASGTKVWLAGCDPRLL
                     VEVGDPLPGAVVKEPLRVPKPEPLVLTMKLLERTEDAEESLSEPKVAFRSDSGFSTKS
                     GFEAGPKDAFSQVAEIHGLPLELRAEDESLLFAVFAAVTRTPAAGGASLVARRAETFR
                     LQMGKPLRWTIDSRWDGGRAAIVLELLVERMPARR"
     gene            complement(13012..13368)
                     /locus_tag="P5164_00470"
     CDS             complement(13012..13368)
                     /locus_tag="P5164_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42390.1"
                     /translation="MSCPDFDDLDDAALAAHLAACPACARLARRDALLADPLVAAQTA
                     AGRFRRARRRAASGLLAASLVAVALLAVALARRPEPPVVYVLEGDETGVVLSGPDLVR
                     RAESLPPSRPMKGDRL"
     gene            complement(13365..13895)
                     /locus_tag="P5164_00475"
     CDS             complement(13365..13895)
                     /locus_tag="P5164_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRY42391.1"
                     /translation="MSDLPDELSGAPPAPAVEEALRIGPDLRAFLERRGHGEDARDLV
                     QDLLVLLLSRPGRFGAPAPGPLRSLLFAIAWRIGANASRRRRAATAVTAGEELRATND
                     LDPESQALLSERVRRAAAALESLPEGTRRALRLVADEGLTPAEAARRLGLGEEALRAR
                     LCRGRRRIAAILEEEP"
     gene            complement(13995..14534)
                     /locus_tag="P5164_00480"
     CDS             complement(13995..14534)
                     /locus_tag="P5164_00480"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRY42392.1"
                     /translation="MKLALLPLAAALLAQPAPAQPGPGGAGAPQQQAGPGIAAAPEAR
                     KTPAPPVYDEAADAKADVAAAVAKAKKEKKRVLVTLGANWCGWCRALDRLFTKNETVA
                     AELAAGYVPVKVDVGRMTKNLELAKGWGADPKTGVPLLVVLDGTGKAVKVQETGSLES
                     GKRHDPEKVIAFLKANTGV"
     gene            14671..16410
                     /locus_tag="P5164_00485"
     CDS             14671..16410
                     /locus_tag="P5164_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42393.1"
                     /translation="MPETRAPRSVGVVAAMLLLSLVAFPLAAQPVRSGPLPGPLPLFP
                     PDNWWNVDVSAAPRDPNEAAILAFIGTSRGLHPDFGGDASASGPDIYGMPYLIVPGSQ
                     PLVPVAFDYDDESDPGAPGRPAGYPIPPEAKTEPRWLEGGWPGSSGAGGDKHLLIVDR
                     DNRLLFETWDTRCEPEGSPSCAWRAGSGAVFPLDANLRRPDGWTSADAAGLAILPGLV
                     RYDEVYGSEPIRHAFRFTVRATNGYVFPASHRAGTTPGAPPFGTRLRLKASRDLSEFP
                     APVRRIFEAMKTYGLILADNGSDMYVQGTYDTRWDNDVLNPAFARLKVSDFEVVKLGW
                     QPAGLPADPCEPGPGPSCHEWLLASGARVRGDGGTSWKTDLTAVNGGDSEATLFLRFL
                     GHDSDGRNGPERSFRLAAGRSATWGDLLGSVFGLEEAWGAVQLRSASPDLLVTSQTFT
                     PGGGGTYGQSVPLFAETELIRGGASRTIPAIREDASFRTNLVLANGAEAACDVDVSLY
                     SEGGALLGARRVPLPPLGMRQLSRVVRELGVTAAVTGARLVLSTPTAGGAFAAYASVI
                     DEKTGDPRTLLPR"
     gene            16686..17105
                     /locus_tag="P5164_00490"
     CDS             16686..17105
                     /locus_tag="P5164_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42394.1"
                     /translation="MNSTRKLSALLVGTLFLASMLALPAAAQQQGAGNGGGAKAGTKR
                     QLGPGDGTGAAPAPKDGSGYGTPYRGENPNAGEGAGPGNGTGNRGNGPKDGTGYGRKA
                     GRGSAGAGSCDGTGPRGGAGSGNHGSGGRRGGRGGRG"
     gene            complement(17250..17756)
                     /locus_tag="P5164_00495"
     CDS             complement(17250..17756)
                     /locus_tag="P5164_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRY42395.1"
                     /translation="MGRTTGPVHAREASLGEALDFMKLLWRLDHALQTTSRRMAESVG
                     VTGPQRLALRVVGRFPGITPGETAAVLRLHPSTVTVIVKGLERAGLVERSEDPADRRR
                     TRLCATAAGRKVASATGGTVEEAVRRTLARVPAADLETAARVLSALASELEPTAAGRG
                     SRAGRRRA"
     gene            17859..18809
                     /locus_tag="P5164_00500"
     CDS             17859..18809
                     /locus_tag="P5164_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRY42396.1"
                     /translation="MTPPPAPHRPPSARRVAVFGEALVDELPSGARPGGAPLNVAAHA
                     AAFGLSPLLLTRLGPDPAGLTLRAAMTSRGIPVEGVQSDPTFPTGRAVVTLRGGIPRF
                     EIPAGAAFDAIDGAAAARAVREARPAVVAFGTLALREPASREALREVLDASPAVRLVD
                     LNLRAPWYDGAVVTRALSAAHVVKASDEELDELRRLLCLTLREPEALAADVVRRFRLK
                     ALYVTRGARGAAVSWRSGDVVHRAEADAAGSHDEVVDTVGAGDAFTAVVLLGLVRHWT
                     AEESLRRGVEFAAAVTRIAGATPEEPSFYAPFLAQWPEAS"
     gene            18806..20959
                     /locus_tag="P5164_00505"
     CDS             18806..20959
                     /locus_tag="P5164_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HRY42397.1"
                     /translation="MNGTSDGLYLLHLSLHGLLRGTSPELGRDPDTGGQITYVLELVR
                     ALGRSPRVARVDLVTRLVEDTAAGPDYAVPLEPLGEKARIVRLPFGPKRYLRKELLWP
                     HLPALVDRCVEHLRAQERLPHLIHTHYADAGYVGVRLSQLLGIPLVHTGHSLGRTKRS
                     RYLAAGRAEASLERPFRFSQRIPAEEEVLENARLVLASTRQEVDEQWGAYENFRPGRF
                     AVVPPGVDTSRFSPPSPFRARTSFPEVDRWLREPYRPLILAISRPSPKKNLPALVEAF
                     GRDPRLRETANLAIFAGVREDVAQSDEEAKAVYTDLLLAADRHDLWGSIALPKKHSPE
                     EVPDLYRLAARRRGVFVNAALIEPFGLTLLEAAASGLPVIATEDGGPRDILANCRNGL
                     LVDPLDLNALSRALVESLGDAARWSIWSRNGLRRVRDTYSWDGHVSRYLALCERLLRR
                     ERKALRRRHAALRATPLAHLADAAHLLVTDIDDTLIGDADSLERLLEWRRARAGRVAF
                     GVATGRSFRRTLEILETWGVPIPDVLVTSVGTEIRFAPELRLDRAWEGHIRHGWRRDE
                     VVRALAGAMGLRKQPEENDGPYKASWDVVPGRHVDLAAIQRFLRARGLAARLVFSQGK
                     FLDVLPVRASKGLAVRFLALRLGLPLESFLVAGDSGNDLEMLVGDTRAVVVGNHKPEL
                     EELRGREGVYFATAPYAGGILEGIAHYSFATEAPC"
     gene            20953..23343
                     /locus_tag="P5164_00510"
     CDS             20953..23343
                     /locus_tag="P5164_00510"
                     /EC_number="2.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016157 - sucrose synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005985 - sucrose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose synthase"
                     /protein_id="HRY42398.1"
                     /translation="MLTRIHDFAAVHPDVLAVLVSRWLAEGRDILLRSDLVRGWEETR
                     AVCDRVDLAASPLAEAVAALQEGVLRAPWIALSFRTGPGRWRYARLHADRLVPEEIGV
                     SEFLRFKEGIVPYGRGEEPLELDFGPFGRDLPRLTEPRSIGQGLSFLNRHLASQLFRD
                     GGAGADRIHSFLGLHTLEGRTLLLRASFADTPALRAALRKAHDLLAAADPATPYRELE
                     ERLAGLGFAPGWGATAGRAAETMSLLIDLLEAPSATLLEAFLARVPMISRVLVLSPHG
                     WFGQSGVLGLPDTGGQVVYILDQVRALEREMRARLAEQGIDVEPRIVVVTRLIPDAGT
                     TGCDERLERIDGCRSAVILRVPFREADGSVVRPFLSRFKVWPYLHRFSHEVETEAAAE
                     LGGRPDLILGNYSDGNLVAALVGMRLGVTHATIAHALEKTKYLLSAVHWKEMEGEYAF
                     SVQYTADLFAMNSADFIVTSTYQEIAGTKEAVGQYESYSSFTMPGLYRVVNGVDVFDP
                     RFNIVSPGADEDVYFSFRERERRLAALHPAIEELVLGEPGPEARGRLVDPGKPIVFTM
                     ARLDRIKNLAGLVGWWAHDERLRSLANLVVVGGHVDPDRCADGEEREQARLLHDLLTR
                     HTLDAEVRWIGKRLDRHLTGELYRWVADRRGVFVQPALFEAFGLTVVEAMVSGLPVFA
                     TCHGGPSEIIEDSRSGFHVDPNDGAAATARIAAFLERCAADPAAWDRVSDAAASRVAA
                     RYTWRRYAERIMTFSRIYGFWKFVSGLERQETARYLQTLDHLLLRPRAAAVGGGPG"
     gene            23571..25130
                     /locus_tag="P5164_00515"
     CDS             23571..25130
                     /locus_tag="P5164_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRY42399.1"
                     /translation="MAIPAAAGALLAFLVPLLAPRAGGGLALVRKAYAGDPTTLLDPR
                     TLVGAFGANSLSLGAGVPLGPEGPTVVLTSGFAAFAARWLRLPGRFVRGMVPVGTAAG
                     IAAIFNAPITGVVFALEEIMGAASRGVLGGSLLAAVAAAVVERTFLGGGRLLPAAPAH
                     WGDLRELLGFAILGLLCGAAAGWLPRFVPILGRGLEALLARTGRHALVARGALAGAVA
                     GLLGVVAPETLGIGYPAVSEWLTGGGSGPQAAIAFAAKLAGVSLALAAPLVGGVFAPS
                     LFLGASLGAAAGHLGQHLFPLAHIEPGAWALVGMGAFFAGFLRTPIASVLIVFEVTGD
                     YGLVLPQMLTVVLASVVARRISRETLVEQALAPAERRPRGAPDPLSALKVGDVMTTAL
                     RAPRASMTILEAARVAAGTRHEQYPLVDDAGLLAGVLFARDLDAAVLENRHEATVGSL
                     ALPPVLVLTPERTLDDAALRLARAGESRAPVVASEEEPRLVGFLSSSDLVRARLRVHE
                     EESGPSMADLG"
     gene            25127..26401
                     /locus_tag="P5164_00520"
     CDS             25127..26401
                     /locus_tag="P5164_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY42400.1"
                     /translation="MSRREAGASAVLALLLAAPACRPGGDAAPGGPAVLRMLVPANER
                     PFWLPLAESFAKARPGVRLELEEGPVPTDLRESLTTSALLSRDGTWDLVYLDVTWTSK
                     LAAAGWLLPLDDAFGEAERAAFLPQALAAGVFRGRLYRVPFRTDVGLLYYRADLLAAA
                     GIAPPRTFAELETAARRLQSPPERWGFLWQGAQYEGLVCFFLEVLHGHGGSWIDPETL
                     EVGLDRPEAEAALALLVRARGAGGVSPPGVTAYREEETRHLFQSGRAVFLRSWPYVFR
                     LAQREGSAVAGKIGVLPVPTLSGAPGSGTLGGWGLAVARASRQPEKAIAFVRHATTLE
                     SQRLFCAPTGFAPARRDGYADPELLAANPFLAELGKIHASATARPVVARYAQASDVLQ
                     RHLTGALAGLSTPREALAAAARETRALLGEAP"
     gene            26398..27288
                     /locus_tag="P5164_00525"
     CDS             26398..27288
                     /locus_tag="P5164_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009782503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY42401.1"
                     /translation="MSTVLARRARLERALLLSPVLLLLALVVAVPALRVLLLGFTRTE
                     LADGVVTSWAGLATHARTLGDGRFLSALRNTALFTFLSVLLEVTLGTALALLLDRSFR
                     GRGALRAVVLVPWALPTSVMALAWAWIFHDTFGMANDLLLRLGLIEAPVAWLAQPGTA
                     MAALVVADAWKTTPFVALILLAGLQGIPREVIEAARVDGAGAFARFRRITLPLLVPSL
                     VVATLFRSIQAWGAFDVVWVMTGGGPGGSTETLALYAYQSWFRYLDFGYGAAIAVQGM
                     LVALLLAAVVVRLASRREEA"
     gene            27285..28118
                     /locus_tag="P5164_00530"
     CDS             27285..28118
                     /locus_tag="P5164_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY42402.1"
                     /translation="MRRPVAAAFLALGICLAAAWSLLPAAWQALTAVKPEAQITASPA
                     VWVPSPPTAEHVRSLLERKPFGRYLANSALVSLAATLLALAAAAPAAAALCRMRPRAR
                     SRWLLGLLLASLFPPILLLFPLYEGVQLLGLVNSPLALVLPYAAFGVPLATWVLEAGF
                     RELPREVEEAATLDGLGPLGRLLRVQLPLAAPSVAAAGLLTFIASWNEFLLALSFTTR
                     ESSRTVTAGIASVSGASLYEVPWGQLSAAIVLATLPVVLLVLLFERRISGGLMGGAVK
                     G"
     gene            complement(28218..29159)
                     /locus_tag="P5164_00535"
     CDS             complement(28218..29159)
                     /locus_tag="P5164_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42403.1"
                     /translation="MAHPFTATRVAFAGILAAALALGGCGWKKEIEQLRTDNQKLTQD
                     KQQLEGQLTSAGVESAEMQATLDEVAKSLEELRAKELQIVKSSIEVVQEGKPRSTQRD
                     ELKTEIEAIKKAIQENLQKLARLEQEKKDALAKAKASGAKVVELEGQVTSMERLIGQM
                     KASLEEKTTLITELETKVLGLTQTVEAQTATIAEKETVIEGQTKVINTAHVAIAGQKQ
                     LEAKGVIEKKGAFLGMRGNWVQTGRFDPEIFREIDITAEQAFAIDAPLKKVQVISAHP
                     KGSYELFAADETHTTLKIVDAAAFWSGEKYLVVKYKD"
     gene            29326..30534
                     /locus_tag="P5164_00540"
     CDS             29326..30534
                     /locus_tag="P5164_00540"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRY42404.1"
                     /translation="MSNVRTKRGTIAILTGGGDVPGLNPAIRALTVRALRDGYRVLGI
                     RRGWAGLIELDGAPGRPDGDCVVELSEEIVNKVGRTGGTFLHTSRTRPSHVPRANVPE
                     HLRDRYRDEMNDLTPEVLANVGKLGIDVLIPIGGDDTLSYGVRLHREGVKVVALPKTM
                     DNDVPGTDYCIGFSTCVTRTIEMTHALRTSAGSHERFLVIEVFGRYAGFTALLPTMAG
                     AANRCVIPEHPFDVDRLTELMVADRTTNPSRYAVVLVSEGAKIAGMGEMVFSDQEQDA
                     YGHRKLGGVGDLVAERLRQLSPRHAGGRRVNTISQRLGYLVRCGDPDALDSIVPMAFG
                     NLAMDLILRGTWGRLVCVRNGRYDDVPVEVVTSRKKTVDVAKHYVADRLRPRYEDFGN
                     KPVFIMTSDY"
BASE COUNT         3825 a        11839 c        10762 g         4308 t
ORIGIN      
        1 actgcttcct gaaggtctgg gagaacctcg accggtacga gccgaccgag gcctcgctcc
       61 tcgcctggat cgccaccctc tcccggaacc tctccatcga ccactaccgc aagcggcgcc
      121 gggagaaggg ctttcatttc gtcacggacg acgcgctcgt gtcgcttctc cccggctcgg
      181 acgacccctc ggccgacgcg gtccgtcggg agcggctccg cctcctcctc gatgcgatcg
      241 gccagctccc ggacgagctc gccgaggtcg tccagatgcg cgacctggac ggcctcgatt
      301 accgcgagat cgccgacctg ctcgggctcc ccgacggtac ggtcaagtcg cgcctgaacc
      361 gcgcccgcat ggagctggcc cggatcgtga gggagaggat cggcgcggcg cccctcgttc
      421 gcggcccgct cttcggcggg ctgacgcccc agaccggagg ggccgcatga cggccggcgc
      481 ctccgccccc tgccgccgcg ccgaggccgc gcttctcgcc ggcggcgttc ccgtcccggc
      541 cgagatcggg gaccacgccg ccgcctgcga gacgtgcggg ccgctcctct cgggatgcga
      601 ggagatcgac gcgctcttcg cctccctcgc cgtgccggag cccccccccg cgctttccgg
      661 agcgctcgaa gcgatcggat cggtcgaacc agctcgcgtc gccgtgcgcg agacgctggc
      721 cctcctcgca ccgggagccc tcgccgtccc cgagccctcg cccgcgctcc tccggcggct
      781 cctcgccgtc gccgccacgg ggccgcgacc tgaggccaac gtcgtcgaga tgagccggtt
      841 ccgccgcctg cgcagcgact ggagggtcct cgtcgcggtc gcttacgccg cctgcctcgc
      901 cctggtcgcg atcctcggcg tcgaccccct ctccgcggcg cgcagcgggg cgagcgggat
      961 ggccgcggcc ggcgagcgcg cgatcgccga ggcgcgcgag gtggccgccg acaagctcga
     1021 caccgtcctc gcggcccagc gtcagaagcc tctcacggag cagctcgact acaaggtcta
     1081 ccggacgctc gccatcggca aggcgaagac ctccgcgtgg gccggcatcc tcctcggccg
     1141 catcttcgga acgcgcgtcg atgccgaggc gccggccgcc cctcgccgga gcgaaccgga
     1201 ccccggcagt ttgcgttcct ttgatcgggc cgggacggac accccgctct cgcgagccca
     1261 gcgagcctga ccgaagccca gaaggtggag ccgatgaacg agaacccgaa ccttccgccg
     1321 ccccccgcga acgtcaccgc ggcccctccg gtcgcctacg ttccgcccgc gccgccgccg
     1381 cgccccgtgg cccctcccgg gaagaacccc gcggccgccg ggctcctctc gctcttcccg
     1441 ggcctcgggc acgtctacct ggggctctac aagcgagggg tcgtcttctt cgcgatctgg
     1501 gcgttcttca tcgccgccgt cgaccgcggc cacgacatgc cgttcggcct cctgatcccg
     1561 ttctggatgg tcttcgtcct cattgacgcc gtccggcagg cgcgcgccct gaacgccacc
     1621 ggcatgcccg agcccggctt cttcggcgag gagaagccgt tccgggccga cggcgggctc
     1681 acgttcggaa tcttcctcat cctgatcggc ctcttcttcc tcgccgaccg gatcttcacg
     1741 atcgacctct cgttcctcct cgactggtgg ccggtcctcc tcgtcgcctt cggcggctgg
     1801 caggtcttcg cttacttcca ggagaagaag cggcgcgacg aggtggcggc cggccgggag
     1861 gcgggagcgg tcgagccgta acgccttcga cgggctcgcg cctgcaagga gggaggggct
     1921 tcggcccctc cctttcgctt ttcgatcgct cgtggcccgg gccgaggagg gcggcgcagc
     1981 cgccgcgggg aggctcgtgg cgagcctccc cgattcgttt ggtgcccctg ccgaggggag
     2041 gcggcgcagc cgccgcgggg aggctcgtgg cgagcctccc cgatccatgt cacccgccga
     2101 ggctcgtgac gaggacgacc gtcgtctcgg aaaccttgcc gtcccgcggg tcgatcaggt
     2161 ggtcctccac gacgacctgg agcgtctcgt cgtccttcgt ccagcgcgcc gtccgacccg
     2221 acttccccgc ccacgccttc tccgcggagg cgtcgacggc gaggccggcc gcgcgcgccc
     2281 actcccgcag gtcgccgagg atgtcgcgct cccccttcag gaagatgagc tgcatcggcg
     2341 ccccctcgta gaaggcgacc gtctcgtccc atccccgggg ggtggtgtag accgcgagcc
     2401 ggagccccgc ggcgcgccgc cggatctcgg ccttcgcctc gggcggctgg acagaccgct
     2461 cgagctcggc gaggaccgaa ttcatctccg ccttcgcgcg gtcggagacg accctcgtca
     2521 tctcaggctc gaaccgcccg ccgggcgggg gcgggacccc tttcaccggc gccgggtcgg
     2581 cgagccagaa gagcgtcagg agtgcgagca ggggcgccat cgcgggcgag tttacccggc
     2641 cgccccgccg tcgcgcttct tcacgacgat ctccgcgggc tgccccgcga ggtagcggag
     2701 cgagcactcg gcgaacccct cgcagcggag gcagacgagg tccttgtccc ccttgaagtc
     2761 gaccggcttg cagatcttca cgcccggtac gacgagcttc tgcagctcgc gccggagccc
     2821 ttcgcctccc ggcggctcga cgcccgagcg ctccgtctgc cggagcgcct cgacgcgcgg
     2881 gaagagctcg cggttcctct tctcgagcgc ctcgtccttc tgctgcacgg ccgtcgtctt
     2941 caccgcggcg accgtctcga gcgccgcctt cctcgccttg aaccggccgt ggtgcctcac
     3001 ccagaagaac aggaaccccg cccccgcgcc caggatgcgc acgaacgccg gcccggcgcc
     3061 gaacgtcacg aggtcgcgga agaacgccag gcccaggagc gccgcgctga agacggcgat
     3121 ccacttcacc ttcaccggga tgacgaagaa gacgaggaac tgcgtctcgg ggaagaggaa
     3181 ggcgtaggcg aagagcatcg agatcgggac gacgaacggg tcggagacga gcggcgtgcc
     3241 gaggacccag gccgagagcg acgccccgag cgtggcgacg agccagaagg ccgtgtactc
     3301 ccccgtcccc cactcggcct cgagcgcgct cccgaggatg tagagcgcga acgccccgaa
     3361 gaagagcgac atcggaccgc cgtggaggaa ctggaacgtc acgaagcccc acggctgcga
     3421 gagcgcctgc tgcggccaga agccgaagag ggccgcggtc ttctgcgggt ccatcgtgaa
     3481 gaggaagaag aagacgaagt ggaggccgca gagcggcatc gtgaggccca tcttcggcgc
     3541 gaagaggtcg cggcccgagg tcatccggag ctccttccga agcgcttcag cacctcggcg
     3601 tgggcgagcg tgaggaggat ggggcgcccg tgggggcagg tccacgggtc gcggcaggcg
     3661 gagaggtcgg cgaggaggcg cgcggcctcc tcgggggcga ggcggcggtt cacggtgatc
     3721 gccccgcggc aggcgagcga ggcggcgagg gcgtccttca tccgcgcccc ggtgtcgccc
     3781 gcgcgtccgg cgcccgtccc ggacgcctcg aggagccggc cgaggaggtc gcggaggacc
     3841 gcgacgacct tctcgggcgc gacgtcggcc ggggccgccg agacgacgaa catccgcccc
     3901 gcgagctccg agacggagaa gccggccgcg acgaggaccc cgtcggccct ccggagcgcc
     3961 tcggcctcct ccggcgcagc ctcgaacgag acgggtgcga ggagcgcctg cgaggcgagc
     4021 ccctgcccct cgagccggtc gcggatccgc tcgaagcgga cccgctcgtg ggcgacgtgc
     4081 tggtcgacga ggacgagccc cccctccccc tcggccacga ggtacgagtc gcggtactgc
     4141 ccgaggagcc ggagcgtccc cgcgggggac ggcacggcgg cggcggccgg cgcgccgccc
     4201 cacgagccgc gcgacggcgc ggggacgacg ccgggggcgg cgaagcccga cgcgcctgct
     4261 aggcccgaag ccccgtccca gagtccgagc gtctcggcga gcgccgcggc cgcgggaggc
     4321 gcgtcgcccc agccgcccgc gcggatcggg attccgtcgt cgggccggtc gcccgtcccc
     4381 gcgagcgccg agcccggcag ctccgtggcc gactttccct ccgcgagcgc ggcgcggagc
     4441 ccccggtgga cgagcgcgtg aacggagccg ggatcgagga agcggacctc gcttttctga
     4501 gggtggacgt tcgggtcgac cctctcgggc gggcaggaga cgaagagcgc ccaggccggg
     4561 tgccgctcgc cgaggagcgc ctcctcggcc gcgaggcgcg ccgcgtgcga gaccgagccg
     4621 tcgcgcgccg cgcggccgtt cacgaagagc cactggaacg tgcgggacgc gaacgtcgtc
     4681 tccggcggag agacggctcc gaggagcctc atccccgcgt gggcgaagtc gacggcgatc
     4741 acgcgcggca gcgtcccccg ccccatcacc tccgcgaagc ggtcgagggc ggccgacgcc
     4801 gccggcaggg cgaggagctc gcgctccccg gcccgcagcg tgaacgccac gtcggggcgc
     4861 gagagggcga gcgacgtcag cgccttcacg acggccctct gctcggcgtc ctgcgacttc
     4921 aggtacttcc ggcgggccgg gacgttcccg aagaggtcct ccacgacgac gcgcgttccc
     4981 ttcgtctggc gcacgggccg cgcgaccggc gcctcgcccc ggtccgcgac gacctccgtc
     5041 ccgagccccg agccgtcggg cgaggtcgcg aggacgaggc gcgagacgga cgcgatcgac
     5101 gggagcgcct cgcctcggaa gccgaggctc ccgatccgcc cgaggtcttc cgccgtccgg
     5161 accttcgacg tcgcgtgccg ctccagcgcg agccgggcgt cgtccgcgtc catcccgcag
     5221 ccgtcgtcgg acaccgagat ccgggcgatt ccgcccccct cgatctccac gtcgacccgc
     5281 cgcgccccgg cgtcgagcgc attctcgacg agctccttca cgacggacgc gggccgctcg
     5341 accacctcgc cggcggcgat ctggtcgacg aggacgtccg ggaggaccgc gatccgcgcc
     5401 atcggccgcg gagagtagca ggggggcgat cggggtactc tcgggccatg gagcggtccc
     5461 ggcgcctctc cccggccctc ttcgtgctcg cggcgctcgt cctcgcgatc ctcgtggcgg
     5521 cgtccgtcct cgtcggccgc gtggcgcggc tcccgaggga gatggccggg gcggtcccgc
     5581 acggcatccg cgagaccgcc gcggccgtct cggacggcct cgcgaggctg gccgcggcga
     5641 tccggacgcg gagcgtgacg accgagctca ggagcgccgt cgccgagctg cgcgggacga
     5701 agaagctcca ggtggcggag ctgacgcagc tcgaggtcgt cgagaggcgc gacgcgacgc
     5761 gcctcctcgg cgtcccgctc cccgacgtcg tcgtctcggc gcgcgcgccg gtcacgtacg
     5821 tctacacgct cgacctcgac gcgcgctggg acttcgacct cgcggagagg accgtgagcg
     5881 tcctcgcccc ggacctcgtc gcgaacgcgc ccgcggtgga cgtctcgaag ctcgagctcg
     5941 tgaagacgga gtcgtcgatc ctccgggacg aggaccgcgt cgtcgaggag ctgcgccgtt
     6001 ccctcacgga ggttttccgg gcgcgggccc ggcagcacgt cccgctcgtg cgggagaccg
     6061 cgcggaagga gaccgaggcg ttcgtgagga gctggctcgt ccgcgcctac gggctccccg
     6121 aggacgtccg ggtcgtcgtc cggttccacg gggagtccgc gaccgtcggc ccggtgggcg
     6181 tcgccctccc gccgcggggc tgacgcgacc gcagaagcta ggtggcgacg acggacgcct
     6241 cggcggccgg ccggcggaag gacagggcga gcgcgccgag cgggacggcg agaaagagga
     6301 gcgccacgag gaacgcgagc gtcgtcaggg cgacgcccgt cgtcgcggcg accttcacga
     6361 ggtcgttctg ccccgtgatc gcgaccgtga ggtagtcggt ccgcgcagcg gcgctgaagg
     6421 cgacgccgcc gaagagcttc acgttccgcg cgacgacata gaacgcgaag aaggcccaga
     6481 cgacgcgctc ccactccggc cgctccgacc ggacgacgcg ctggaaagcc cacgccgcga
     6541 acgcggcctc cgcgaggtgt ccgccgaggt cgaaggccgt gacgtgcgcc ttcgtgaagg
     6601 cgaagagggg gtagaggagg gcgacggctg cgaagacgag gttccaccgt ggcgcccggc
     6661 ggtacctcac ggcgaggaag acgaggccgc cccagacgag cgccgcgacg ccccggttct
     6721 gctcgaagac gacggtcatg acgatcgccg gcagggccgt gcagccgaag aaccaggcga
     6781 cgaacgtgtg cccgagctcg tgcacgaaaa aggagatgcc ctcgcccatc acgcggaacg
     6841 ggaagaacca tccgaggagg acggacggca cgaaggcgac gaggagcgcg cggactccgg
     6901 gcgcgagggc ggcccaggcg gcggcgacgc cctcggcacc ggtcgcccgg ggcctcggcg
     6961 gcggcggagc gggcggcggc tgggcgacac cgggagcggc gggcccgggg gagctcgacg
     7021 ccgcgatccc gcccgctccg ccggaatctc ttggggggtc ggccgccggc aaagcggcct
     7081 cgctctcggc ggtcccgccc gcctcgccgt actccaagac gctccggacg aggttccgct
     7141 cgtcccacgg ggcgaggacg tgacgccagc cgccgcccgc gcgcggctcc acgcggagga
     7201 cctcccagag atcgccgtcc caccggacgg ccgtgcccgg gcgaacggga gcgcgcgggc
     7261 cgccctcctc ccgccccgac catcctttcg gccggagcga gaggaggacg acggtgccgt
     7321 cggtcgtcgg ctcgaggcgg tcgcggccgg tggggcgggg cgccacgcgg agacgttacc
     7381 ccggaccggc cgcggcgcga agcctcactt cagcggcgcg tggaactccc cggaaagctg
     7441 ggtccacttg tccttgacgt cgaacttccc ggagatcgag tcggcggtca gcgaagtgac
     7501 ctcgatcgtc cccgtgggct gcgagagaga aagggacgtc ccgcccgtca cggcgatcga
     7561 gacgctcgcc gtcatctcgg cctgcccctg ggacttcgtc gggtcgtaca tcccggcgac
     7621 gaactccagc gtctcgcccg gcgcgacctg cttctgcctc accagaccga gctcgacccg
     7681 tccctggccc ggctcgggcg aagtcctctt gcggtactcg gccatcgtcg tcggggcgaa
     7741 gtcgtagttc gtgaggacga cggtgaccga cgcgaccccg accgagacgt ccttgaacgc
     7801 gatcgctcgc ggcgaagcga agtcgggggc gttctccttc ggccaggcgc cgttcgtcga
     7861 cgtgaccgtg accttcgtga cccccgcggg ggcggccgga gccggagctt ccggcgcggg
     7921 agcctcggcg acgggagcgg cctccggggc cgcgggcttc tcggcgggct tgccgcaggc
     7981 ggcgacgagg caggctcccg ccaggacgga cagtgcgatc gttctcatcg aggtcatggg
     8041 atcctcctgc gttcatcctc ccgccggatc gcgcaggatg caattgatgg gcgtcaccgc
     8101 ccgatcgtcc aatctgagac cagttcacaa agtaccgccc cgctgcggcc gaagccggag
     8161 cggggcggcg aggtctccga ggggagggcc tacaggcgcg ggaggaacgc cgtcaggtag
     8221 gtccagaagt tcttcaccga cgggatgctc acgcactcgt cgggagagtg ggcgttgcgg
     8281 atcgtcgggc cgaaggagat cgtgtccatc ccgggatact tctcgccgat gatcccgcac
     8341 tcgaggccgg cgtggatcgc cttcacctcg ggctccttcc cgaagagctc ggcgtggacc
     8401 tccttggcga ggccgaggag cggagaggtc acgttcggct tccacccggg gtagccgtcg
     8461 ccctgcttca cctcgaagcc ggcgaggccc atggcggtcg ccatcatccg gccggcgtcc
     8521 ttcttcgccg actcgacgga gctgcgctgg ctcgtcgaga aggagacgac gtcgtccttc
     8581 gtctcgacga tcgcgaggtt cgtcgaggtc tgcacgaggt tcgggatgtc gggggaccag
     8641 cccaggacgc cgtgcggcgc cgcgtggatg aagccgacga tccgcgcaag gtccgcctcg
     8701 gcgatcggat cgggagcggc gtcgatccgc tccaggccga cgttcaccgg gttgtcgaag
     8761 cgccccgcct cggagcggac gtccgcctcg agcttcgcga gcgccgcgcg cacttccggc
     8821 tccttcaccg ggtcgagcca gccgaccgcg aagccctcgc gggcgatcgc gttccgcttg
     8881 ctcccgccgt cgagcgtcgc ggcggcaaag ccgaccgcgc cggcctcggt gacggcgcgc
     8941 gcgagcacct tcagggcgtt cacgcgcccc tcgtggatgt cgcagccgga gtgcccgccg
     9001 cggagccccg tgaccgagat ccggtacggc gagagggcgg cggtcggctt cgtccgctcg
     9061 accttcgtcg tggcgagggt gtcgaccccg ccggcgcatc cgacgtagac cgccccctcc
     9121 tcctcggtgt cgaggttgag catccggcgg ccggtgacga aaccgggcgc gaggacgttc
     9181 gcccccgtca tccccgtctc ctcgtcgatc gtgaagaggc actcgagggg cgggtgggcg
     9241 aggtcgttcg actcgaggat cgcgagcgcc gcggcgcagc cgatcccgtt gtcggcgccg
     9301 agcgtcgtcc cgtcggcctt gacgacgtct ccgtcgacga cgaggcggat cgggtccttc
     9361 gtgaagtcgt gggcggtgcc cgagttcttc tcgcagacca tgtcgacgtg gccctggagg
     9421 acgacgacgg gcttcgactc gaggcccttc gtcccgggct tcgtgatgac gacgttcccg
     9481 acctggtcga ccttcgcggg gaggccgagg cgggcggcga ccgcgacgac gtgggccgag
     9541 gcggcggctt cgttcttcga gccgcgaggg atcgcggcga gggcagcgaa gtggtcccag
     9601 agcagcttcg gctcgagacc ggagaggggg tgggtcatga gggggtctcc ttcacgaggc
     9661 cggagaggcc ggcccgtgat catagccggt cgcgagctcg cgggcgttgg acgacgatcc
     9721 cgccccgggg agagcgccgg gccccggccg aggccgcccc ggcggccctc ggcgcccgtc
     9781 accgagcgtc ccgtacgatc cgctccggag gtcccgatgc gccgtcccgc tctcctcgcc
     9841 ctgacgctcc tcgccgcgct cccgctcgcc gccgagcccg cggcgaagcg ctccctcacc
     9901 gtcgacgaca tctggtccgt cgttcgcgtc ggcgcgccgg tcgtctcgcg ggacgggacg
     9961 gtcgccgtct acccgcgttc cgtctgggac gcggagaaga accggctcct ctccgacctc
    10021 tggctgcagc cggtcgcggg cgggccggga cgccggctca ccgcgcacga ggcgggcgag
    10081 tcctcccccg cgatctcgcc cgacggctcg cgcgtcgcct tcgtcgcgaa gcgcgacggc
    10141 gacgaggtcg cgcagctcta cgtcctccgc ctcgacggcg gcgaggcgga gcgcctgacc
    10201 gacctccccg ccgctcccgg ctcgccccgc ttcacggcgg acgggaagcg gatcgtcttc
    10261 tcggcgaacg tcctcggcga gagcctcgag acgacgaaag cggagctcgc gcgccggaag
    10321 aaggaggaga agacccgcgt cctcgcgacc gtctccgaac gccggctcgt ccgctactgg
    10381 gaccgctggg tcgagcagga cgagtggccg cacctcctct ccgtcgacgt cgcgacgcgc
    10441 gtcgtcgccg acctgacgcc cgggctgaag aggctcgggc gcctcgaggg cggcctggag
    10501 ttcgacgtcg cgcccgacgg ctccgtcgtc ttcgccgcga acgcgaccga gcccccgttc
    10561 cggacgctga acaccgacct cttcctcgtc ccggccggcg gcggcactcc gcggaacctg
    10621 acggccgaca accccgccga ggactcctcc cccgtcttct cccccgacgg gaagacgatc
    10681 gcctatgggc gcgaggcgaa gggcgacggc tggcccgacc ggacgcgtct cgccctcctg
    10741 gacgtcgcga gcgggaagac gaaggtcctc acggagagct gggatcgcgt cccccagggc
    10801 tggagcttca cccccgacgg ccggacgctc gtcttcctcg ccgaggaccg cgcccggacg
    10861 cacgtctggt cgctgccggt ctcgggcggg acgccgaagg tcgtctggaa gggaggccgc
    10921 gcgaacgccc tcgaggtcgc agggccggcc ggactcgtct tcacctacac gacgttccgc
    10981 cggccggccg agcttgccac ggcgaagctc gacggctcgg gcttccgcaa cctcacgtcg
    11041 tggaacgacg ccttgatgga gcagatcgcc ttcggcgagg agcgcgaggt cgtcttcaag
    11101 ggagccggcg gcgacgacgt gcagatgttc gtcgtccatc ctcccggctt cgagaagggg
    11161 aagacgtacc ctctcgtcca cgtcgtccac ggcggcccga tcggcacctc gggcgacgag
    11221 ttccacctcc gctggaacgc ccacgccttc gccgctcccg gctacgtcgt cgcgctcgtc
    11281 aacttccacg gctcctcgag cttcggccag gcgttcgtcg agtcgatcct gggcgccccg
    11341 ggagacaagc cgttccagga cgtgatggcc gcgaccgact tcctcgtcgc cgagggctcc
    11401 gtcgacccga agcgggtggc ggccgccggc gggtcgtacg gcggctacct cgtgaactgg
    11461 atcgccggcc agaccgaccg cttcgcggcg ctcgtcagcc acgccggcat ctacagcctc
    11521 ctcggccagt tcggctccga cctcgcctac ggccgccagc actcctacgg cgcctacccc
    11581 ttcacgaacc cgtcggcgat cgagcgctgg agcccgaacc gctacgccgc gagcctgaag
    11641 acgccgatgc tcgtcgtcca cggcgagaag gacttccgcg tcccgatcac gcaggggctc
    11701 gagctctacg gcgtcctgac cgccaagggg gtcccggctc gcctcgtcgc ctaccccgac
    11761 gagaaccact gggtcctcaa ggggacgaac gcgaagcact ggtacggcga ggtcctcggg
    11821 tggctcgcga ggtggctgaa gtagcgccgc tcacgccctc ccggtgaccc ggtcgagctc
    11881 gttgcagggc ggctgccggc gctcctcagc gccgcgccgg catccgttcg accaggagct
    11941 cgaggacgat cgccgctcgc ccgccgtccc acctcgaatc gatcgtccag cggagcggct
    12001 tgcccatctg cagccggaac gtctccgccc ggcgcgccac gagggacgcg ccgcccgccg
    12061 ccggcgtcct cgtcaccgcc gcgaagacgg cgaagagaag gctctcgtcc tccgcgcgca
    12121 gctccagcgg cagtccgtga atctcggcga cctggctgaa ggcgtccttc ggtcccgcct
    12181 cgaagccgga cttcgtgctg aagccggagt cggagcggaa ggcgactttc ggctcggaga
    12241 ggctttcctc ggcgtcctcc gtcctctcga ggagcttcat cgtcaggacg agcggctccg
    12301 gcttcgggac gcgaaggggc tctttcacca ccgctccggg cagcggatct ccgacctcca
    12361 ccagcaggcg gggatcgcag ccggcgagcc acactttcgt cccggatgcg gcggcttcgc
    12421 cgaggatcct ccccttcgca cctccacccg aaggatcgac ggcggcgagg gcgatgccat
    12481 ccgtcgctcc ccctctcgcc ccgaggcggc cgagagtcag gggctcggcg cagccgggca
    12541 cttcgacccg gccggcgaag tcggggacac ggaccgcgac cgggggggcg ccggcgccgt
    12601 cgagccagaa gcggaggact ccgccgagcg ggacgggatc ggacgtccgc gccgcggggg
    12661 ccgtcgccgc cccggtcgcc ttcgaagccc ccttcgctcg tgcgcggagg accttcgacg
    12721 agaccgtgac gtcgacgttc gcggcctggc cgatcatctc gagagccttc cgaagcggca
    12781 cgttcgagat ctcgacgctc acgcggccgc cgacgccggg gtccaggatc ggcgtgacgc
    12841 cgagcaggtc ggcgagcttc tcgaggacgt cgacgacgga agcgtccttc agctgcaggg
    12901 cgatggggac gtcgaggcgg tcctgcgtgt ccatctcgac cgggacgggc gcgggtgcgg
    12961 cttcaacggc gggcgcgccc gcgagggccg ccgcgacgag aacgggaagg atcacagccg
    13021 gtctcctttc atcggtcggg acggcggaag cgattcggcc cggcggacga ggtcgggtcc
    13081 gctcaggaca acgccggtct cgtccccttc cagaacgtag acgaccggcg gctcgggccg
    13141 gcgcgcgagc gcgacggcga ggagcgcgac ggcgacgagc gaggcggcga gaaggcccga
    13201 ggccgccctc cgccgcgcgc ggcggaacct cccggcggcg gtctgggcgg cgacgagcgg
    13261 gtcggcgagg agcgcgtccc gccgcgccaa gcgggcgcag gccgggcacg cggcgaggtg
    13321 cgccgcgagg gcggcgtcgt cgagatcgtc gaagtcgggg cagctcacgg ttcctcctcc
    13381 aggatcgcgg cgatgcggcg gcggcctcta cagagccgtg cgcggagcgc ctcctccccg
    13441 aggccgagcc tccgcgcggc ctccgccggg gtcagccctt cgtcggccac gagccggagc
    13501 gcgcgcctcg ttccctcggg gagcgactcg agcgccgccg cggcgcgccg caccctctcc
    13561 gagaggaggg cctgcgattc gggatcgagg tcgttcgtcg cccggagctc ttctccggcc
    13621 gtcaccgcgg tcgcggcccg gcgccgccgc gaggcgttcg ccccgatcct ccacgcgatc
    13681 gcgaagagga gggagcgcag cgggccgggc gccggcgcac cgaaccgtcc cggccggctg
    13741 aggaggagga cgaggaggtc ctggacgagg tcccgggcgt cctcgccgtg accccggcgc
    13801 tccaggaagg cccggaggtc cggaccgatc cgaagcgcct cttcgaccgc gggagccggc
    13861 ggcgctccgg agagctcgtc gggaaggtcg ctcacgacac ggtaatgtcg cacggcggcg
    13921 tcgcgtgacg ccgccgtgcc gggagccgcg aggaaccggg gagagacgcg cggacgacgc
    13981 ggcccgcggg ttcgtcagac cccggtgttc gccttcagga aggcgatcac cttctccggg
    14041 tcgtggcgct tgccggactc gagcgacccg gtctcctgga ccttcacggc cttgcccgtg
    14101 ccgtcgagga cgacgaggag cgggacgccc gtcttcgggt cggctcccca gcccttcgcg
    14161 agctcgaggt tcttcgtcat ccggccgacg tcgaccttga ccgggacgta acccgccgcg
    14221 agctccgcgg cgaccgtctc gttcttcgtg aagagccggt cgagtgcgcg gcaccacccg
    14281 caccagttcg cgccgagcgt cacgaggacc cgcttcttct ccttctttgc cttcgccacg
    14341 gccgcggcga cgtcggcctt cgcgtcggcc gcctcgtcgt agaccggcgg ggccggggtc
    14401 ttccgggcct cgggcgccgc ggcgatcccg ggtccggcct gctgctgggg agctccggcg
    14461 cctcccggac ccggctgggc gggggcgggc tgagcaagga gggcggcggc gagagggagg
    14521 agggcgagct tcatgacagg ggagttctac ggacgtccgg ccgtccgcgt tgcgcgtgcg
    14581 ccgggtcacg ccgaggtcgg ttcggggtcc ggcccgaccc ggcggacccg ggacggcgtt
    14641 aggtgcggac cacttccaga cggagggacc atgccagaga cccgcgcgcc ccgatcggtc
    14701 ggcgtcgtcg ccgccatgct tctcctctcg ctcgtcgcgt ttcctctcgc ggcccagccg
    14761 gtccggagcg ggcccctccc cgggccgctc cccctcttcc cgcccgacaa ctggtggaac
    14821 gtcgacgtga gcgcggcgcc gcgcgacccg aacgaggcgg cgatcctcgc gttcatcggg
    14881 acgagccggg ggctccaccc cgacttcgga ggggacgcct cggcgagcgg cccggacatc
    14941 tacgggatgc cctacctcat cgtcccgggc tcccagccgc tcgtgccggt cgccttcgac
    15001 tacgacgacg agagcgaccc gggggcgccc ggccggcccg ccggctaccc gatcccgccg
    15061 gaggcgaaga cggagccgag gtggctcgag gggggatggc ccggcagctc gggcgcggga
    15121 ggggacaagc acctcctgat cgtcgaccgc gacaaccggc tcctcttcga gacgtgggac
    15181 acccgctgcg agccggaggg ctccccgtcc tgcgcctggc gggccggctc cggggccgtc
    15241 ttcccgctcg acgcgaacct gcgccgcccc gacggctgga cgagcgccga cgccgcgggg
    15301 ctcgcgatcc tcccgggcct cgtgcggtac gacgaggtct acgggagcga gccgatccgc
    15361 cacgcgtttc gcttcaccgt ccgcgcgacg aacggctacg tcttccccgc ctcccaccgg
    15421 gcgggcacga cccccggcgc gcccccgttc gggacgcgtc tccggctgaa ggcttcgcgc
    15481 gacctctcgg agtttccggc gccggtgcgg cggatcttcg aggcgatgaa gacctacggc
    15541 ctgatcctcg ccgacaacgg ctcggacatg tacgtccagg ggacctacga cacgcgctgg
    15601 gacaacgacg tcctcaaccc ggcgttcgcg cgcctgaagg tctccgactt cgaggtcgtg
    15661 aagctcgggt ggcagccggc cggcctcccc gccgacccgt gcgagccggg gccgggtcca
    15721 tcctgccacg agtggctcct cgcgtcgggc gctcgggtgc gcggcgacgg cggaacgagc
    15781 tggaagaccg acctgacggc cgtgaacggc ggcgattccg aggcgaccct cttcctccgc
    15841 ttcctcgggc acgactccga cggaaggaac ggcccggagc gctccttccg cctcgcggcc
    15901 ggccgctccg cgacctgggg cgacctcctc ggctccgtct tcgggctcga ggaggcgtgg
    15961 ggcgccgtcc agctccgctc cgcgagcccc gacctcctcg tcacgagcca gaccttcacc
    16021 cccggcggcg gcggcaccta cgggcagtcg gtccccctct tcgccgagac ggagctgatc
    16081 cggggcggcg cgtcgcggac gatccccgcg atccgcgagg acgcctcctt ccggacgaac
    16141 ctcgtcctcg cgaacggagc cgaggccgcg tgcgacgtcg acgtctcgct ctactccgag
    16201 ggcggggccc tcctcggtgc gcgccgcgtg ccgctcccgc cgctcgggat gaggcagctc
    16261 tctcgcgtcg tgagggagct cggcgtgacc gccgccgtca ccggggcccg gctcgtcctc
    16321 tcgacgccga ccgcgggagg cgcgttcgcc gcctacgcct cggtcatcga cgagaagacg
    16381 ggcgatccgc ggacgctcct gcctcggtag cgggcgggct cgccgacggg cggcctcccc
    16441 gaccgccgcc gggaggtcgc ccgggctgcc ggagctttcg acctgcagcg cctcgcgctg
    16501 ccctgcaacg tctgcagcca cgccacggcg gcgtcgcccc ccctgctgac ccgcaacgtc
    16561 ctgccccgtg cggatttccg cgctccagag gattccgggc cgctcgtggc acggagcttg
    16621 tgaagggggg gtgcgagccg gacgccgaag aggccgagcc ggacgacccg aaaggagtca
    16681 cgacgatgaa cagcacccgg aagctctcgg ccctcctcgt gggcacactc ttcctcgcca
    16741 gcatgctcgc cctcccggcc gccgcgcagc agcagggggc cggaaacggc ggcggcgcga
    16801 aggccggtac gaagcggcag ctcggccccg gtgacgggac gggcgccgca cccgccccga
    16861 aggacggatc cggctacggc accccctacc gcggcgagaa cccgaacgcc ggcgaaggcg
    16921 cgggccccgg caacggaacg gggaatcgcg gcaacgggcc gaaggacggc acgggctacg
    16981 gaaggaaggc cgggcgcggc tcggcgggcg ccgggagctg tgacgggacc gggccgcggg
    17041 ggggcgcggg ctccggcaac cacggctccg gcggacggcg cggcgggcgc ggcgggcgcg
    17101 gctgaggccc gaccacatcg cacggtccgt tccgcgggcg gtcccggcag ggctggggcc
    17161 gcccgttctc gcgggcccct ctccgtaggc ctcccgagag ggacgtctca cccggggcgt
    17221 cggcctcttt ccggccgctc gccccctcgt caggctcggc gccggcccgc ccgcgacccc
    17281 cgcccggccg cagtcggctc gagctccgac gcgagcgccg agaggacgcg cgcagcggtc
    17341 tcgaggtcgg ccgccggcac ccgcgcgaga gtccgtcgca ccgcctcctc gaccgttccg
    17401 ccggtcgcgg aggcgacctt ccgcccggcc gccgtcgcgc agaggcgcgt ccgcctccgg
    17461 tccgccgggt cctccgagcg ctcgacgagc ccggcccgct cgagcccctt cacgatgacg
    17521 gtcaccgtgc tcgggtggag gcgcaggacc gccgccgttt cgcccggggt gatccccggg
    17581 aagcggccga cgacgcggag cgccaggcgc tgcggtccgg tcacgccgac cgactccgcc
    17641 atccgccgcg aggtcgtctg gagcgcgtgg tcgaggcgcc agaggagctt catgaagtcg
    17701 agcgcctcgc cgagggaggc ctccctcgcg tggaccggac ccgtcgttct ccccatcact
    17761 cctccgcgcg gccgcgggcc gcgccccgat ttcaccacgc cccgattgtt tgtggtcaaa
    17821 tattttgatc tcaaatcatt ttccggtagg atctccccgt gacgcctccc ccggctcccc
    17881 atcgtccgcc ttccgcccgc cgcgtcgcgg tcttcggcga agcgctcgtc gacgagctcc
    17941 cctcaggcgc gaggccaggg ggggcccccc tgaacgtcgc cgcccacgcc gcggccttcg
    18001 ggctctcccc cctcctgctg acgcggctcg gcccggaccc ggccgggctg acgctccggg
    18061 cggcgatgac gtcgcgcggc attcccgtcg agggggtcca gtccgacccg acgttcccga
    18121 cgggccgcgc cgtcgtgacg ctccgcggcg gcattccccg cttcgagatc cccgccggcg
    18181 ccgctttcga cgcgatcgac ggcgccgccg ccgcccgcgc cgtgcgcgag gcgcggcctg
    18241 cggtcgtcgc gttcggcacg ctcgccctcc gcgagccggc ctcgcgcgag gcgctccgcg
    18301 aggtcctcga cgcctcgccc gcggtccggc tcgtcgacct caacctgcgc gccccctggt
    18361 acgacggcgc ggtcgtcacc cgcgccctct cggcggcgca cgtcgtgaag gcgagcgacg
    18421 aggagctcga cgagctgcgc cgcctcctct gcctgacgct ccgggagccg gaggctctcg
    18481 cggccgacgt cgtccgccgc ttccggctga aggccctcta tgtcacccgc ggcgcgcggg
    18541 gagccgccgt ctcctggcgc tcgggcgacg tcgtccatcg tgccgaggcc gacgccgccg
    18601 gctcgcacga cgaggtcgtc gacacggtcg gagccggcga cgccttcacg gccgtcgtcc
    18661 tcctcggcct cgtccggcac tggacggcgg aggagagcct ccggcgcggc gtcgagttcg
    18721 ccgcggccgt cacaaggatc gccggcgcga cgccggagga gccgtcgttc tacgcgccgt
    18781 tcctcgcaca gtggccggag gcgtcgtgaa cgggacatcc gacggcctct acctcctcca
    18841 cctgagcctc cacgggctcc tgcgcgggac gagccccgag ctggggcgcg accccgacac
    18901 cggcggccag atcacctacg tcctcgagct ggtccgcgcg ctcggtcgct cgccgcgcgt
    18961 ggcccgcgtc gacctcgtca cccgcctcgt cgaggacacc gccgcgggtc ccgactacgc
    19021 cgttcctctc gagcctctcg gcgagaaggc gcgcatcgtg cgcctcccgt tcggcccgaa
    19081 gcggtacctg cgcaaggagc tcctctggcc ccacctcccc gcgctcgtcg accggtgcgt
    19141 cgagcacctg cgggcgcagg agcggctccc gcacctgatc cacacgcact acgccgacgc
    19201 cggctacgtc ggcgtccggc tctcccagct gctcggaatc ccgctcgtcc acacggggca
    19261 ctccctcggc cggacgaagc gctcccgcta cctcgccgcg gggcgcgccg aggcgagcct
    19321 cgagcgcccc ttccgcttct cgcagcggat cccagcggag gaggaggtcc tcgagaacgc
    19381 gcggctcgtc ctggcgagca cgcggcagga ggtcgacgag cagtggggcg cctacgagaa
    19441 cttccgcccc ggccggttcg ccgtcgtccc tcccggtgtc gatacctcgc gcttctcccc
    19501 gccgtccccg ttccgggcgc ggacgtcgtt ccccgaggtc gaccggtggc tccgcgagcc
    19561 gtatcgccct ctcatcctcg ccatcagccg cccctcgccg aagaagaacc tcccggccct
    19621 cgtcgaggcc ttcgggcgcg acccgcggct gcgcgagacg gcgaacctcg cgatcttcgc
    19681 cggcgtgagg gaggacgtcg cccaaagcga cgaggaggcg aaggcggtct acacggacct
    19741 cctcctcgcg gccgaccggc acgacctctg gggctcgatc gcgctcccga agaaacactc
    19801 cccggaggag gtcccggacc tctacaggct cgcggcccgc cggcgcggcg tcttcgtgaa
    19861 cgcggccctg atcgagccgt tcgggctcac gctcctcgag gcggccgcga gcggcctccc
    19921 cgtcatcgcg accgaggacg gcggtccgcg agacatcctc gcgaactgcc ggaacggcct
    19981 cctcgtcgac ccgctcgacc tcaacgccct ctcgcgcgcg ctcgtcgagt cgctcggcga
    20041 cgccgcccgc tggtcgatct ggtcgcgcaa cggcctccgg cgcgtccggg acacctactc
    20101 ctgggacggc cacgtctccc gctacctcgc cctctgcgag cggctcctgc gccgcgagcg
    20161 caaggccctc cgccgaaggc acgccgccct gagggcgacc cccctcgccc acctcgccga
    20221 cgccgcgcac ctcctcgtca ccgacatcga cgacacgctg atcggcgacg ccgactcgct
    20281 cgaacggctc ctcgagtggc gccgcgcgcg cgccggccgc gtcgcgttcg gcgtggcgac
    20341 ggggcgctcg ttccgaagaa cgctggagat cctcgagacg tggggcgtcc cgattcccga
    20401 cgtcctcgtc acctccgtcg gcaccgagat ccgcttcgcc ccggagctcc ggctcgaccg
    20461 ggcgtgggag gggcacatcc ggcacggctg gaggcgggac gaggtcgtcc gagcgctcgc
    20521 gggcgcgatg ggcctccgca agcagcccga ggagaacgac ggcccctaca aggcgagctg
    20581 ggacgtcgtc ccgggacggc acgtcgacct cgcggcgatc cagcgcttcc tccgggcgcg
    20641 cggcctcgcg gcccgcctcg tcttctctca ggggaagttc ctcgacgtgc tgccggtccg
    20701 cgcctcgaag gggctcgcgg tccgattcct cgccctccgc ctcggcctcc cgctcgagtc
    20761 gttcctcgtc gcgggcgact ccgggaacga cctcgagatg ctcgtcggcg acacccgcgc
    20821 ggtcgtcgtc ggaaaccaca agccggagct cgaggagctc cgggggcgcg agggggtcta
    20881 cttcgcgact gctccctacg ccggagggat tctggaaggg atcgcccatt actccttcgc
    20941 gacggaggcg ccatgctgac ccgcatccac gacttcgccg ccgtccaccc cgacgtcctc
    21001 gccgtcctcg tgagccgatg gctcgcggag gggcgcgaca tcctcctccg ctccgacctc
    21061 gtccgcgggt gggaggagac ccgggccgtc tgcgaccgcg tcgacctcgc cgcctcgccc
    21121 ctcgccgaag cggtcgcggc gctccaggag ggggtcctcc gcgccccttg gatcgccctc
    21181 tccttccgga ccggaccggg ccggtggcga tacgcgcgcc tccacgccga ccgcctggtc
    21241 cccgaggaga tcggcgtctc cgagttcctc cgcttcaagg aggggatcgt cccctacggc
    21301 cggggagagg agccgctcga gctcgacttc ggccccttcg gccgcgacct cccgcgcctt
    21361 accgagccgc gctcgatcgg gcaggggctc tccttcctga accgccacct cgccagccag
    21421 ctcttccgcg acggcggcgc gggggccgac cgcatccact cctttctcgg cctccacacc
    21481 ctcgagggcc gcacgctcct cctgcgcgcg tcgttcgccg acacgccggc cctccgcgcg
    21541 gcccttcgca aggcccacga cctcctcgcc gccgccgacc ccgcgacgcc gtaccgggag
    21601 ctcgaggagc gccttgccgg cctcggcttt gccccgggtt ggggggcgac ggccggacgt
    21661 gccgccgaga cgatgagcct cctgatcgac ctcctcgagg cgccgtccgc gacgctcctc
    21721 gaggcgttcc tcgcccgcgt cccgatgatc tcgcgcgtcc tcgtcctctc tccgcacggc
    21781 tggttcgggc agtcgggcgt cctcggcctg ccggacacgg gcgggcaggt cgtctacatc
    21841 ctcgaccagg tccgggccct cgagcgggag atgcgcgcgc gcctcgccga gcaggggatc
    21901 gacgtcgagc cgcggatcgt cgtcgtcacg cgcctcatcc ccgacgccgg gacgaccggt
    21961 tgcgacgagc ggctcgagag gatcgacggc tgccggagcg ccgtcatcct gcgcgtcccg
    22021 ttccgcgagg cggacgggag cgtcgtccgc ccgttcctct cccgcttcaa ggtctggccc
    22081 tacctccacc gcttctccca cgaggtggag acggaggcgg cggcggagct cggcgggagg
    22141 cccgacctga tcctgggcaa ctactccgac gggaacctcg tcgcggccct cgtcggcatg
    22201 cgcctcggcg tgacgcacgc gacgatcgcg cacgccctcg agaagacgaa gtacctcctc
    22261 tcggcggtcc actggaagga gatggagggt gagtacgcgt tctccgtcca gtacacggcc
    22321 gacctcttcg cgatgaacag cgccgacttc atcgtcacga gcacctacca ggagatcgcc
    22381 gggacgaagg aggcggtcgg gcagtacgag agctactctt cgttcaccat gccgggcctc
    22441 taccgggtcg tgaacggagt cgacgtcttc gacccgcgct tcaacatcgt ctctccgggc
    22501 gccgacgagg acgtctactt ctcgttccgc gagcgggagc ggcgcctcgc cgccctccac
    22561 cccgcgatcg aggagctcgt cctcggcgag ccggggccgg aggcgcgcgg ccggctcgtc
    22621 gaccccggca agccgatcgt cttcacgatg gcccgcctcg accggatcaa gaacctcgcc
    22681 ggcctcgtcg gctggtgggc gcacgacgag cggctccgct ccctggcgaa cctcgtcgtc
    22741 gtcggcgggc acgtcgaccc cgaccgctgc gccgacggcg aggagcgcga gcaggcccgg
    22801 ctcctccacg acctcctgac gcgccacacc ctcgacgccg aggtccgctg gatcgggaag
    22861 cggctcgacc gccacctgac gggcgagctc taccggtggg tcgccgaccg gcgcggcgtc
    22921 ttcgtccagc cggccctctt cgaggcgttc ggcctgacgg tcgtcgaggc gatggtctcg
    22981 gggctcccgg tcttcgcgac ctgccacggc ggcccctccg agatcatcga ggactcccgc
    23041 tccggcttcc acgtcgaccc gaacgacggc gcggccgcga cggcgcggat cgccgccttc
    23101 ctcgagcgct gcgctgccga cccggcggcg tgggaccgcg tgtccgacgc ggccgcctcg
    23161 cgggtcgccg cccgctacac ctggcggcgg tacgcggagc ggatcatgac gttctcgcgg
    23221 atctacgggt tctggaaatt cgtctccggg ctcgagcggc aggagacggc gcgctacctc
    23281 cagacgctcg accacctcct cctccgtccc cgcgccgcgg ccgtcggcgg aggccccggc
    23341 tgagcggact cccgccgtcc agcggcacgg ggccgcgcgc gtggctggag tcgctcctcg
    23401 ggctcctccg gccgcgctcg cgccagggcg tcctcgtcac ggtcgtcctg atcggcgcgg
    23461 gctgcggcct cgtcgcggtc gccttccacg gcgccatcga gctcctccgc gccctattcg
    23521 tcgggacggc gctccggcag acgagcccgc tggcgcgcgg agccctgctc gtggcgatcc
    23581 cggcggccgc cggggcgctc ctcgccttcc tcgtcccgct cttggccccg cgcgcgggcg
    23641 gcggcctcgc cctcgtccgg aaggcgtacg ccggcgaccc gacgaccctg ctcgacccgc
    23701 gcaccctcgt cggtgccttc ggcgcgaact ccctctcgct cggcgccggc gtgccgcttg
    23761 gccccgaggg gccgaccgtc gtcctgacga gcggcttcgc cgcattcgcc gcacggtggc
    23821 tgcgactccc gggacggttc gtccgcggga tggttccggt cgggactgcc gcggggatcg
    23881 cggccatctt caacgccccg atcaccggcg tcgtcttcgc tctcgaggag atcatgggag
    23941 ccgcctcgcg cggcgtcctc ggcggctcgc tcctcgccgc ggtcgccgcg gccgtcgtcg
    24001 agcggacgtt cctcggcggc ggccggctcc tccccgcggc tcccgcccac tggggcgacc
    24061 tgcgcgagct cctcggcttc gccatcctcg gcctcctctg cggcgccgcg gccggctggc
    24121 tcccccgctt cgtcccgatc ctcggccgcg gcctcgaggc gctcctcgcg aggaccgggc
    24181 gccacgccct cgtggcacgc ggcgccctcg ccggcgccgt cgccggcctc ctcggcgtcg
    24241 tcgctcccga gacgctcggc atcggctacc cggccgtctc ggagtggctg acaggaggag
    24301 gcagcgggcc gcaggccgcg atcgcgttcg cggcgaagct cgccggcgtc tcgctcgccc
    24361 tcgccgcgcc gctcgtcggc ggcgtcttcg cgccgtcgct cttcctcggt gcctcgctcg
    24421 gcgccgccgc cgggcacctc ggccagcacc tcttcccgct cgcgcacatc gagccggggg
    24481 cgtgggccct cgtcgggatg ggggccttct tcgccggctt cctccggacg ccgatcgcct
    24541 cggtcctcat cgtcttcgag gtgaccggcg actacggcct cgtcctgcct cagatgctga
    24601 ccgtcgtcct cgcctcggtc gtcgcgcggc ggatctcgcg cgagacgctc gtcgagcagg
    24661 cgctcgcccc cgccgagcgg cgcccgcgcg gcgcgcccga cccgctctcc gcgctgaagg
    24721 tcggagacgt catgacgacg gccctccggg ccccgcgcgc ctcgatgacg atcctcgaag
    24781 cggctcgcgt cgccgccggc acgcgccacg agcaataccc tctcgtcgac gacgccggcc
    24841 tcctcgccgg cgtcctcttc gcgcgcgacc tcgacgcggc ggtcctcgag aaccggcacg
    24901 aggcgaccgt cgggagcctc gccctcccgc cggtcctcgt cctgacgccg gagcggacgc
    24961 tcgacgacgc ggcgctgcga ctcgcccgcg cgggcgagtc gcgcgccccg gtcgtcgcgt
    25021 cggaggagga gccgcggctc gtcggcttcc tctcgtcgtc cgacctcgtc cgggcgcgcc
    25081 tgagagtcca cgaggaggag tcgggcccct cgatggcgga cctcggatga gccgccgcga
    25141 ggccggcgcc tcggccgtcc tcgcgctcct cctcgcggcc cccgcgtgcc ggccgggcgg
    25201 ggacgccgcg cccggcggcc ccgccgtcct ccggatgctc gtccccgcga acgagcgccc
    25261 gttctggctt ccgctcgcgg agtcgttcgc gaaggcccgg ccgggcgtcc gcctcgagct
    25321 cgaggagggg ccggtcccga ccgacctgcg cgagagcctg acgacctcgg ccctcctctc
    25381 gcgcgacggc acctgggacc tcgtctatct cgacgtcacc tggacctcga agctcgccgc
    25441 ggccggctgg ctcctcccgc tcgacgacgc cttcggcgag gcggagcgcg ccgcgttcct
    25501 cccgcaggcg ctcgcggcgg gcgtcttccg cgggcgcctc taccgcgtcc ccttccggac
    25561 cgacgtcggc ctcctctatt accgcgccga cctcctcgcg gcggcgggga tcgccccgcc
    25621 gcgcaccttc gcggagctcg agacggccgc gcgccggctc cagtcgcctc cggagcgctg
    25681 ggggttcctc tggcaggggg cgcagtacga ggggctcgtc tgcttcttcc tcgaggtcct
    25741 gcacggccac ggtgggagct ggatcgaccc ggagacgctc gaggtcggcc tcgaccgccc
    25801 cgaggccgag gcggccctcg cgctcctcgt ccgcgcgcgc ggcgccgggg gcgtcagtcc
    25861 tccgggcgtg acggcctacc gcgaggagga gacgcgccac ctcttccaat cgggccgcgc
    25921 cgtcttcctc cggagctggc cctacgtttt ccggctcgcg cagcgggagg ggtcggccgt
    25981 cgcggggaag atcggcgttc tccccgtccc gacgctctcg ggcgcacccg gctccggcac
    26041 tctcggaggc tggggcctcg ccgtcgcgcg cgcctcgcgc cagccggaga aggcgatcgc
    26101 gttcgtgagg cacgcgacga ctctcgagag ccaacggctc ttctgcgcgc cgaccggctt
    26161 cgcccccgcg cgacgggacg ggtacgccga ccccgagctc ctcgccgcga acccgttcct
    26221 cgccgagctc gggaagatcc acgcctccgc caccgcgcgc cccgtcgtgg cccgctacgc
    26281 ccaggcctcc gacgtcctcc agcggcacct caccggggcg ctcgcgggtc tctcgacgcc
    26341 gcgcgaggcg ctcgccgccg ccgcgcgcga gacgcgcgcc ctcctcgggg aggcgccgtg
    26401 agcaccgtcc tcgcgcgccg cgcacggctc gagcgggcgc tcctcctctc gcccgtcctc
    26461 ctcctcctcg ccctcgtcgt cgccgtgccg gcgctccgcg tccttctcct cggcttcacc
    26521 cggacggagc tcgcggacgg cgtcgtcacc tcctgggccg gcctcgcgac gcacgcgcgg
    26581 accctcggcg acggccgctt cctctcggcc ctcaggaaca ccgccctctt cacgttcctc
    26641 tccgtcctcc tcgaggtgac gctcgggacg gcgctcgccc tcctcctcga ccgctccttc
    26701 cggggccggg gcgcgctccg ggccgtcgtc ctcgtcccgt gggcgctccc gacgtccgtc
    26761 atggcgctcg cgtgggcctg gatcttccac gacacgttcg ggatggcgaa cgacctcctc
    26821 ctccgcctcg gcctgatcga ggcgcccgtc gcctggctcg cgcagcccgg cacggcgatg
    26881 gccgccctcg tcgtcgccga cgcctggaaa acgacgccgt tcgtcgcgct catcctcctc
    26941 gcggggctcc aggggatccc gcgcgaggtg atcgaggccg cgcgcgtcga cggggcaggg
    27001 gccttcgccc gcttccggcg gatcaccctc ccgctcctcg tcccgtcgct cgtcgtcgcg
    27061 accctcttcc ggtcgatcca ggcctggggc gccttcgacg tcgtctgggt gatgacggga
    27121 ggcggcccgg ggggctccac ggagacgctc gccctctacg cctaccagag ctggttccgc
    27181 tacctcgact tcggctacgg cgccgcgatc gccgtgcagg ggatgctcgt cgccctcctc
    27241 ctcgcggcgg tcgtcgtgag gctcgcctcg cgaagggagg aggcgtgagg cgccccgtcg
    27301 ccgcggcgtt cctcgcgctc ggaatctgcc tcgcggcggc ctggagcctc ctcccggcgg
    27361 cgtggcaggc gctgacggcc gtgaagccgg aggcgcagat caccgcctcg ccggccgtct
    27421 gggtcccgag cccgccgacg gccgagcacg tccgctcgct cctggaacgg aagccgttcg
    27481 ggcggtacct cgccaacagc gccctcgtct ccctcgcggc gacgctcctc gccctcgcgg
    27541 ccgccgcgcc cgccgccgcc gcgctctgcc ggatgcgccc ccgcgcgcgg agccggtggc
    27601 tcctcggcct cctcctcgcc tccctcttcc cgccgatcct cctcctcttc ccgctctacg
    27661 agggagtcca gctcctcggc ctcgtcaact cgccgctcgc gctcgtcctc ccctacgccg
    27721 cgttcggcgt cccgctcgcg acgtgggtcc tcgaggcggg cttccgcgag ctgccgcggg
    27781 aggtcgagga ggcggcgacg ctcgacggcc tcggccctct cgggcggctc ctccgcgtcc
    27841 agctcccgct cgcggcgccg tccgtcgccg cggccggcct cctcacgttc atcgcgtcgt
    27901 ggaacgagtt cctcctcgcc ctctccttca cgacgcgcga gtcgagccgg accgtcaccg
    27961 ccggcatcgc gagcgtctcg ggcgcgtcgc tctacgaggt tccgtggggc cagctctcgg
    28021 cggcgatcgt cctcgcgacg ctcccggtcg tcctcctcgt cctcctgttc gagaggagga
    28081 tctccggggg tctgatgggg ggcgcagtga agggctgaac gcgacgaggc ccgccggggc
    28141 ctcccccggc gggcctcgcg ggaatccgga gcggggcccg gcgctcccct cccggggcgc
    28201 gccggacccg cgacgcgtca gtccttgtac ttgacgacga ggtacttctc gcccgaccag
    28261 aacgcggccg cgtcgacgat cttcagcgtc gtgtgcgtct cgtcggcggc gaagagctcg
    28321 tagctcccct tcgggtgcgc cgagatgacc tggaccttct tgagcggcgc gtcgatcgcg
    28381 aacgcctgct cggccgtgat gtcgatctcg cggaagatct cggggtcgaa cctcccggtc
    28441 tgcacccagt tcccgcgcat gccgaggaag gcgcccttct tctcgatgac ccccttggcc
    28501 tcgagctgct tctgcccggc gatcgccacg tgcgcggtgt tgatcacctt cgtctgcccc
    28561 tcgatgacgg tctccttctc cgcgatcgtc gccgtctgcg cctcgacggt ctgcgtgagg
    28621 ccgaggacct tcgtctcgag ctcggtgatg agcgtcgtct tctcctcgag cgacgccttc
    28681 atctggccga tgaggcgctc catcgacgtg acctgcccct cgagctccac gaccttggcg
    28741 ccggaggcct tcgccttcgc gagcgcgtcc ttcttctcct gctcgaggcg ggccagcttc
    28801 tggaggttct cctggatcgc cttcttgatc gcctcgatct cggtcttcag ctcgtcgcgc
    28861 tgggtggagc ggggcttccc ttcctggacg acctcgatcg agctcttcac gatctggagc
    28921 tccttggcgc ggagctcctc gaggctcttg gcgacttcgt cgagcgtcgc ctgcatctcg
    28981 gcgctctcga cgccggccga ggtgagctgc ccctcgagct gctgcttgtc ctgggtgagc
    29041 ttctggttgt cggtgcggag ctgctcgatc tccttcttcc agccgcagcc gccgagcgcg
    29101 agggcggcgg cgagaatccc ggcgaaagcg acccgtgtcg cggtgaacgg atgggccatg
    29161 gcgttcctcc tctgagagtg cgatcgagtt cgtcggatga cccgtaagac taccgcggag
    29221 ccggaccgcc ggcgtcaact tcctgccccg cccctccttc gggcgcccgg cgccggccga
    29281 gagcggcggt gagatgatcc ctgcccgaaa agggagcaga agccgatgtc gaacgtgcgg
    29341 acgaagcgcg gaacgatcgc gatcctgacg ggcggagggg acgtcccggg cctgaacccc
    29401 gccatccggg ccctgacggt ccgggccctc cgggacggct accgggtcct cgggatccgg
    29461 cggggctggg ccggcctgat cgagctggac ggggccccgg ggcgccccga cggcgactgc
    29521 gtcgtcgagc tctccgagga gatcgtcaac aaggtcggcc ggaccggcgg gaccttcctc
    29581 cacacctccc ggacccgccc ttcccacgtc ccccgggcga acgtccccga gcacctccgg
    29641 gaccgctacc gggacgagat gaacgacctc actcccgagg tcctcgcgaa cgtcggaaag
    29701 ctcggcatcg acgtcctgat cccgatcggc ggcgacgaca cgctctccta cggggtccgc
    29761 cttcacaggg agggggtgaa ggtcgtcgcg ctcccgaaga cgatggacaa cgacgtcccg
    29821 gggaccgact actgcatcgg cttctcgacc tgcgtgacgc gcacgatcga gatgacgcac
    29881 gcgctccgga cctccgccgg gagccacgaa cggttcctcg tcatcgaggt cttcgggcgg
    29941 tacgccggct ttaccgccct cctcccgacg atggccggcg ccgcgaaccg ctgcgtcatc
    30001 cccgagcacc cgttcgacgt ggaccggctg accgagctca tggtcgccga ccggacgacg
    30061 aacccgagcc ggtacgccgt cgtcctcgtc tccgagggag ccaagattgc cgggatgggg
    30121 gagatggtct tctcggacca ggagcaggac gcctacgggc accggaagct cgggggcgtc
    30181 ggcgacctcg tcgccgagcg gctccggcag ctctctccgc ggcacgcggg cggccgtcgc
    30241 gtgaacacga tcagccagcg gctcggctac ctcgtccgtt gcggcgaccc cgacgcgctc
    30301 gattcgatcg tcccgatggc cttcgggaac ctcgcgatgg acctgatcct ccgcgggacc
    30361 tggggccggc tcgtctgcgt gaggaacggg cggtacgacg acgtccccgt cgaggtcgtg
    30421 acgagccgca agaagaccgt cgacgtcgcg aagcactacg tcgccgaccg gctccggccc
    30481 cgctacgagg acttcgggaa caagccggtc ttcatcatga cgagcgacta ttgagcggtg
    30541 tcgggctctc ccgtgcgatt ccgtcgtaac ctccgggcgg agccgagcac gtgatacgcc
    30601 tctacggatt cgtcgacggg acccttcggg ccctccacct cgcgccgctt ggcgaggcga
    30661 gcggccccga gtgggacgcg gtcgcctggg tcgacctcct cgatcccgag cccgaagaga
    30721 aggcctgggt cgag
//
LOCUS       DAOTEZ010000005        27718 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_1121_length_27718_cov_6.172758, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000005 DAOTEZ010000000
VERSION     DAOTEZ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 27718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27718
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1121_length_27718_cov_6.172758"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="P5164_00545"
     CDS             <1..601
                     /locus_tag="P5164_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRY42405.1"
                     /translation="ADPEPAYHGLSFSAFAPAAYAARLRTVLLRDVGAAVSPFNSFLF
                     LQGLETLPLRIRRHADNALAVARHLESHPAVAWVRYPGLASHPTHGTAARVLSGGFGG
                     VLTFGVKPPAGVSAEQAARSFIANLSLFSLVANVGDARSLVIHPWTTTHEQLTEEERL
                     AAGVSPDLVRLSVGIEEPEDLLADLDAALARLGGTERTR"
     gene            598..1365
                     /locus_tag="P5164_00550"
     CDS             598..1365
                     /locus_tag="P5164_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42406.1"
                     /translation="MSARIETLVVKFGGTSLGTPARIRRAARRVAALVDSGRRVVVVV
                     SAMGHTTDQILETLGALEAGRTEAAAREVDRALATGEDLSAALLAAALNTLGVPARSL
                     RGGEAGVRADGALGAARVARVDARRLRGLLARGVVPVVSGFQGERDDREVATLGRGGS
                     DTSAVAIAAALGPAPCHIVTDVDAVYDRDPRRHVDARPLPRLSHGELLALARSGAQVV
                     AADAARLAAEQGVPLRVLGYDAAPNDERSGTFVGGAR"
     gene            1362..2417
                     /locus_tag="P5164_00555"
     CDS             1362..2417
                     /locus_tag="P5164_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRY42407.1"
                     /translation="MSGPPDVAALRVGLAEHRAGDFALESDETLRDVRQAFRLEGEID
                     ADGGNVVLLFHSLTGSPADLGGWRPFVGPDLPIDTRRFAVLAPNLLGSCYGTHFLRGA
                     GHAKRPLAVTTRDQARLAGLLLDALGVKRLALVAGGSLGGMVTLELCATRPDAARAAI
                     VFAAPASPTAWGAAWNHVHRTALEAVPRSGLALARMVGMLTYRTPIEVERRFRPHGSE
                     THPVRDYLDHHGEKLLRRFSPRAYRTLLDAIDAHDVGRGRGGVGAALRPFRGRLVGVG
                     IPGDLLYPPEEVLRWTRAAGAEYRELASRSGHDGFLLETGAVGLLLAEALSVGGAGRS
                     PAPAPLLAANLDAGPPP"
     gene            2417..3898
                     /locus_tag="P5164_00560"
     CDS             2417..3898
                     /locus_tag="P5164_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42408.1"
                     /translation="MPARRAARSPVASLVLAAGLAVVGCGKGRFAPSPVALPEGKVWV
                     DHLSRDLAPFWLRPEALGSPVGAFPTFRCDDGSLVDLELPCGELLVAGTWVSSRLGVE
                     YTRMRSRQTFFYGVAFHLTGEERYLALAKAGVDHLRAHALDRAERSAVSWWKDGVAGP
                     PVPARSAQDLAYANLGLAFWAYLTRDPDVLADALLLYEGIFDRYDDPERGLLRWAAGG
                     VGGETARVELVAQLDQVNAYALLLASFAPEPHRSRLRADLARIARVLIDRFWSAELGL
                     FHGSLHDPVERGPGARHTDFGHTVKALWMIERTGALLGDEALSSWARERLPGLLEKAY
                     LPRYGCWATGLRLDGSLDAGVTWWTAAELDQAAATLALTDSGYTGYLEKTWTCWLDRL
                     VDHENGEVWSWIDLDRPERRGAKQHLWKNGYHSAEHALVSYLVAQELHGRPATLYFAP
                     AEGTALGTFRPYFFSGAATVGPERRLESIPSRRSIAVSFRGLR"
     gene            3928..4998
                     /locus_tag="P5164_00565"
     CDS             3928..4998
                     /locus_tag="P5164_00565"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY42409.1"
                     /translation="MTSDAQRLAARISDATLAFQDVLTIYLGERLGLYDDLERNGPST
                     SAQLASRTGTHERYVREWLEQQACAGILGCAEPGAPAGERRFFLTTASAEVLTDRDSL
                     LWSAARPRSLVALAQKLPMLREAFRSGRGITDGMEDARTAQGDLGRAAFLRQLATEWL
                     PAVPDVDARLRAEPPARVLDVGCGSGWASIALARGYPLARVDGLDLDPAAVEDARANA
                     RTHGVEDRVAFHACDAAAPELREGYDLVIAFEAIHDMPRPVEALAAMRRLASPSGTVL
                     VVEMKVGESFALPPPPNDRLNYGWSVLTCLSSSMCGEEPAGTGTLLRRGTLESYARRA
                     GFSAIEDAGVPHEGWTFWRLLG"
     gene            5054..5797
                     /locus_tag="P5164_00570"
     CDS             5054..5797
                     /locus_tag="P5164_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42410.1"
                     /translation="MKTERTGPAAFTVEFESEAELEAEVRTNLSAGGLRLPTREKLAI
                     FAKLQLLLRLAGGGAVPATATVVAPLPDGFALHVEGDAAVLADALRDAPEEPGEEVLE
                     EAGDAEDPVARESVWDRLRGLTRVEKLLLAAKADRVERMILAQDSDPQVLYSLLKNPR
                     LTADEVIRIAKSPFLTFQTAEAMMKSTQWFSNLEVRVALVHNAKTPPAFALRILPTLP
                     ESEVRNISRGAATSMALKVAALKKLQGFT"
     gene            5871..7508
                     /locus_tag="P5164_00575"
     CDS             5871..7508
                     /locus_tag="P5164_00575"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRY42411.1"
                     /translation="MPEPAIPSLSEAAAALGLPPERLVPYGHDKAKVLPSPGGFTPRG
                     RLVLVSAITPTGAGEGKTTTSIGLVQGLARIGARACVALREPSLGPTFGMKGGATGGG
                     RARLVPSDDINLHFTGDFHAVTSAHNLLAALLDNHVHHGNRLDIDPRRVFWRRVLDMN
                     DRALRDVVIGLGGVLQGVPRETGFDITPASEVMAALCLAEDAEDLRARLSRIVVALTF
                     GKEPVTAADLGATGAMLALLRDALLPNLVRTGEGVPAFVHGGPFANIAHGCSSVIATK
                     TALSLADWVVTEAGFGFDLGAEKFFDIKCAGAGLDTAAVVLVATVRALKRHGGVPKEG
                     LTKPDPGAVERGLPNLAKHLESIRAFGEVPVVALNRFTTDSEEEVSVVRRAAEGWGAP
                     FAVCDAFEKGGEGAVALAEAVLSHAERKSSLFRPLYAREDSPKEKLAKIARHMYGAAS
                     VEWSREAERDLAEIARLGYEKLPLCVAKTQMSLSDDPGVVGRPEGFDVTVRGVLLAAG
                     AGYLVPLLGDILRMPGLPSAPQAERIDLVGGRIVGIS"
     gene            complement(7501..9078)
                     /locus_tag="P5164_00580"
     CDS             complement(7501..9078)
                     /locus_tag="P5164_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRY42412.1"
                     /translation="MTRWPLRLVAAVLLAVLLPPALLVWLSFRLESRALRDDLERRAR
                     SAAALTDALLAGAESLLTRLGSRWDGSCAPETAEALRAALELGSGVRGVALLDAELHP
                     ACSAGTAPAPLPPSVRTALEGDARAVWEGGAPGDLLRLALRLPFGAAEASLDAGPIAR
                     ALSELAGGGRDAVEVRGVAGPVLALPGSREPRRLALPLRLLGPLVGARDETAAAPCAG
                     GGASVLLRASREAQLAAWGATLPLLVAIGLGFGAGAAALVLLAGSPGAALTRQLRGAI
                     RRGELEVRYLPIVEVTSGRCVGAEALLRWRHAREGLLSPDLFIALAEETGLIVPITEW
                     LMRRVARDLPDGLREPDRFYVAINLAGAHLRDPGTVAAVLRAFAGSRLGPKSLVFEVT
                     ERELIDDANGTARNVLEALQGWGATIALDDFGTGYSNIATLRRFRLDHLKIDKSFVEG
                     IGTGSVAETVVDTLVELGRKLEMTVVAEGVEREEQVDYLVERGVRHFQGFLYAKPLPL
                     KELVAFVEGRGGGPPVS"
     gene            complement(9151..10938)
                     /locus_tag="P5164_00585"
     CDS             complement(9151..10938)
                     /locus_tag="P5164_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42413.1"
                     /translation="MTDPVDEAPAERDPATFEEARERLRARGYLDRGVEGAVLKGALA
                     ARTRTRALLRGAALASLLLALALAVVETASVALASALSPRDALVLFAWLGAGALVAAS
                     GLVLALGAVAWLRTRGRSDPSRASTEVAVGFGLLAGVGAALAARPVLDAAGPVAAAVA
                     FGAAALAVVLAVRVARGLAFTVLAASGRSVLSRAPRPALALGTALAAIAVATGAWLLA
                     RRPEPPAEPLVVRAGTARVVLVAVDGWLPELAGDAARLAAASRFSYRKETADPAAFWT
                     TVATGEPARRHGVGALDLVRLDGVTAPVKLTAGTGWYLGRLLPALGAAREESVTSAAR
                     RVPAIWEVAHRGGLASLTVNWWTTWPAGDAGGVVLGNHLFFAARAGRPLEGEGWPAEA
                     AARAAAAAPRAAPGADGVARLVDDARGLDAFAIAAFREEAARSRPRLSLLYLPGLDVL
                     GAALSEPGRSVGERAALAAALADEARDVASFLAGPDVLGSSAEIVALVLDPGRSGRDG
                     ELLVAGPLAAEGASGGFRPVDLAPTLLAALGVPASRQTEGRVDSRLVRAGAATSATVA
                     SWGGRPRGGAAPIDPKEYVESLRSLGYLR"
     gene            complement(10979..11878)
                     /locus_tag="P5164_00590"
     CDS             complement(10979..11878)
                     /locus_tag="P5164_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRY42414.1"
                     /translation="MPTSLPETLARVRRVVDDRLPGLLADPASGPDAAAVGDALPGAA
                     REALLSPGKRVRPGLVFLVGDLFGAPEQRLLDPACAVEMIHAASLALDDLPSMDDATT
                     RRGRPALHAVHGEDLAILAAVTLIVRAFGVLADAGLSALRPATALDLVSRLADATGLL
                     GLASGQSLDLRTPEAAASFDRLETIHARKTGALFVASAEFGAVLGGARERELAAVRSY
                     ARNVGLAFQIVDDLLERQDPARTGKHAKRAPAPTFARHVGEEGARRLVTELTAHAVEA
                     LEPFGARGALLREFASLLGARTA"
     gene            11945..15142
                     /locus_tag="P5164_00595"
     CDS             11945..15142
                     /locus_tag="P5164_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY42415.1"
                     /translation="MPAASTKTSSRAREWLSALLAPAVVLLGFAALALARGPSPLRIL
                     TTGLLTAAAVAALGRLASGRLLRSREATRDALHRASAGDLTLSRAALARSGENALAEA
                     LHGLMVAMERILASFARLSAAVSGVAADLTTRGRELSLAAAMQSSRAEETADAMRGTD
                     AAIGSLRESMETLAGAAENAGASLHEMTASVTQVSRSTTALRAFVDETTASLGGVVSA
                     LQQVASAVENLSGLADETARATAAIRSSTAETDRQAQTGARLSERVSAAVVSGKTAVS
                     GTLAGMHEIRAAVSGAADASTALAEHSARIGEILHAIEEIAGETNLLALNASIIAAQA
                     GESGKTFSVLADDIRDLSERTAVSTEEVRGLVSSVRGGVEDVRRLLSDARRRTEEGVD
                     LAQTSDATLDEIEALALESRRASEGIAGRAVAQSADAARVSEATGRVSAEVVRISEAT
                     RGQLETTRTVHARADRVRELTEQLARAMEEQAVGGQTLLGSMERVTSTVDAIAEATVT
                     LADGSSAVVGSMDEIRRAARQNAYAATAMNQAAQALEQESLLLRSRSSLFRLPSPAPG
                     GRIRAALRYLDEANFDPAFSQTVPQAILARTWGEGLVKFGEGTRIVPELAERWEIDPT
                     GTLFTFHLRRGVRWHEGGTLSAADVKASFERFLSPATGAPLAALFDAVEGYEEFRAGR
                     RPGVAGLETPSGSVLNVRLSRPVPFFLQLLTLPDITVLPPAILGDPDRIRRFPCGTGA
                     FVPREIAFGKSARFDRFDGYWERNRIAPDGIDLDLAEDSEAGVFQRFLDGQLDVVWDI
                     PYPEAARLMADPAWRPYIDSSVQLHTSFLVLRCDRAPLDDVRVRRALNHAVDRAALNA
                     RTFAGLSVPAASILPPHVVGHDPALRPYRHEPETAKALLREAGHGDGLTLSAWLSPKD
                     ARDPLNPLPVIASQLEAVGVRLALEILPGEEAAARKRNGVFPDVRLARWFADFPDPDT
                     FFRSLFSSKAESVLDGGYANAEVDRLVEKGAGLLDAAEREALYRELDRLVQSEAPLVF
                     LFHNRGFVAHAPRLRGVQAHLLLPPVRWNELWLEP"
     gene            complement(15239..15586)
                     /locus_tag="P5164_00600"
     CDS             complement(15239..15586)
                     /locus_tag="P5164_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015608329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRY42416.1"
                     /translation="MNKNASIRKAGEVTIIELKGKITIGAGDLQMREAIHAALAEGAR
                     KLVVDMSQVVTIDSSGVGELVGCFTTATHKGAKMKLMNLPAKISDVLTVTQLITVFDV
                     YPNEAEAIASFSA"
     gene            complement(15583..16038)
                     /locus_tag="P5164_00605"
     CDS             complement(15583..16038)
                     /locus_tag="P5164_00605"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY42417.1"
                     /translation="MPAAAPGPIVLWLSSRYENIELAQAALAHVCRLRGIDAEMEHWI
                     GMAMREAVANAVKHGNRLDPDKRVLVRLESAGDDLTIVIGDEGGGFDPERISDPLAPE
                     NQLKTSGRGIFYIRTFMDDVTFSRGEGGGTVLTMRKDLSKRGDAKGEKP"
     gene            16118..17419
                     /locus_tag="P5164_00610"
     CDS             16118..17419
                     /locus_tag="P5164_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42418.1"
                     /translation="MCAPEPCDDVAEIIRDSGRRRPLSRPSLSVVGVLGLAALATVTA
                     PPARSDESALLAVRVAKAQKLVEKVRGVSFRAPVASALLPERELETVLAKKLVQDLPV
                     PFDDYAAGLAALGLIEPSPGLLEKLTRLYTRQVVGFYDPAEKRFYIVPERSRDAGGPA
                     GDLMEQLLLAHELTHALQDQRLGLDRRMKTLRDSTDSLLALQAFLEGEATVLMTEALL
                     ESVPEEAREALGADPLGQVLEGLDDPDGVEGADGVPAYFVRELVFPYAAGTAWVRQKK
                     SAGGWRAVDTAYRRLPTTTREILRPGVALSPRLRLAPADRPTPGLVPGGGNASWADTL
                     GEWVLGTLLEQAGAGDASRDAAAAWQDDRIVFSYPVASPGAHRVGFLWRIRAASPQAA
                     ARIAELLEPIYAARPAPARPRVSVRGDVVEVACPAVPPPPA"
     gene            complement(17423..18202)
                     /locus_tag="P5164_00615"
     CDS             complement(17423..18202)
                     /locus_tag="P5164_00615"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HRY42419.1"
                     /translation="MKPIDLFLLLGVGLPVVAAIVVVAARRGAFRRDEFPHERRRAGA
                     LVALGVTLAATTILPAFSLGRPIDPARLSLPSILALQQLLLVFLIGWWLLAGRPEAAE
                     FLALTAPDPRREVRTGLVVGLAGWGLTVAAGLVVLGVLRFAGMDEVRPIPPLVRWLAS
                     QPLASRLVLILAAMTVEEVFFRSFLQRRLGAVPASILFLVSHGGYGDPLYFVGLLAIT
                     AILAATFEKTGSVLAPMVAHGTFDAVQLLLVLPSAVRAIPG"
     gene            complement(18437..18513)
                     /locus_tag="P5164_00620"
     tRNA            complement(18437..18513)
                     /locus_tag="P5164_00620"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18477..18479),aa:Asp,seq:gtc)
     gene            complement(18607..21390)
                     /locus_tag="P5164_00625"
     CDS             complement(18607..21390)
                     /locus_tag="P5164_00625"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HRY42420.1"
                     /translation="MRARFLLVALLVLAAWPARGEELWGRRIESLGFRGDAPIEEASF
                     ARLTDLGPGQLLTEGAVHSSLRNLFATGRFADLAVEAAPAAEGAAVTIVFSAAARVAE
                     VEVAGERIPVKGRVLDALKTRPRDAWSEERGRTATRSALRVLEERGYFEAEVTPWVSP
                     GPDETSVNVRFEVVAGRPAAPAPAEFTGDLGPLAADDLRRKGKLRREGVFREGVARED
                     AERYAALYRELGRSRAEVRYEGVSYDRGSGRATARYAVFAGPDVVLDVTGIAEAVVRR
                     HPASPWAKGDPPDADALARLRSALREELQGKGYARAEVDVDVEVSADREEVRLHAERG
                     ERWVVGRTFVEGVCAIPERTLLAVLRTRPRGLFEKGRLVDPDLAADRAAIASVYRARG
                     FPAAKVTAEVFAGALPYELDVRFRVDEGPAAWIGRVSVDGLATLDVAAISRRLRSRPG
                     GRFLQEDVDADLTALRSALGDGGFPDGRVEARAARRAAEPGAPVVVDVVYEVSEGPRA
                     VFGKTILRGARATRLSVLERELAYEEGAPFSMARLIRTQQNLDRLGVFSRVDVSGLSP
                     EPGGSTRSILVQVEETKPWSLLYGLGVEYDERAEKAFNPRLSLALTHANLFGRAIAGV
                     VEARYSRRDTRVLLRLADRSVFDSGLAGTITGYVAEEIRQSYGVRRGGAFFEASRVFA
                     GTVKGALRYQYEIVEPDAPPDILSEIERQDQRILINSVGASAVVDRRDDPIVPSKGFF
                     AAAEGKWAFPAAAADAHFLKGSTQLSLYRPFRGGTLAAGVRAGAARSLVTCNPEANPG
                     CLPNLDTPIVERVFAGGRTTHRSFPLDDLGVEGQTLKGGRGIGGNAFLLANVEWRVPL
                     TGGLGVVLFADWGNVWAGPAYVKLPEGRWGAGLGIHYLTPVGPFRLEYGFRLDRKKDE
                     DTGALSFSIGYPF"
     gene            complement(21387..25412)
                     /locus_tag="P5164_00630"
     CDS             complement(21387..25412)
                     /locus_tag="P5164_00630"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HRY42421.1"
                     /translation="MGARKGLGGRIPHPQIPQLSLEQVRVLRLFALLFVFSILVLYAV
                     FRSARFQDLLRRRTQTLLSEKIGREVRIGGFDLALVPPAFLVKDVFVANDPRGLPGSC
                     FSAEEISLRGIPQVSESRIDLPKVRLISPRIVLEVFGDGTNNFSSIAAALPKGGGGGR
                     DVRVRQAVLQKGTIRFREWKSKLDVVLDDAALTARSNRFSRTTRASLGCRKARFRLDD
                     GDVLDLEVGADVVLTPGRLRLRGLRLRGDGLALDALGGIDDLKDPEVKVLARVRARGE
                     DLDRYFGAGVPLSGPVSAVASVRVPRGGGYRIRGRFEIEEGGSFGPFPMTGEGFLRVD
                     PEGLLAHVSRATYAGGNLEALVQLARIKGPPLPVRIAVSGREIDFERFFSDLGLSGTG
                     LMARTDLDMTLTFGPGGVERSDGSGVIRLHPAHGVPSAVRGRHALPVSGGGPLFVENG
                     AIRFPGVPFVTAGGLTAVLDGTLRIGSWEPDWKLAIDAPDLREAERLAENFYPAIQGE
                     PLLPKLNLGGSGRLVATLARSFSDPRIAGRLEASEFVLRGVRFGQTAADFVVDRNVLA
                     LDALDARDGDGRMVVRGEVGWGGALGDEYRLSGLTLETERWPAERVLSFLTLDLPIAG
                     RVTGRLPLSGVTPRVVGAGDLVFEETTLWGQPFDRVAGTLGFEAEVLRLSGVSGTLGA
                     GSARLDGLYRYDDDGFEVKAEASALPLERLGALSGEAPGLTGLLTGTLEGSGTLEEPR
                     LSLEGRVAEAAIGGQALGRAGEPLSVRLALDGDDLDAEAEAPGTARLTARGKSRDVSL
                     DLEVLSLGAYSSLLGLVPEAGLDGTLAIDARLRTDPETGLASAQGRVASLDARMGPHR
                     FFLPAPAPFGWSGGRLSWVGARVGARRTAGEGAALPEGEVTIDGSVETGGAGAIDVRA
                     TGTMEAGFLAPFLGVASLEGRMAVSATASGTTARPVFEGRVVLDGLELVTSPGEVPVE
                     AISGTILLTPGRVSTDDLTLRWNGAVAVAGAATLDGLAWTGMRLNVHLDGLRNEPFPG
                     LRSTVSGDLVLLGDDELRSARGELTLTRGLYAEDLDLSLQALLGGRRGAAEAPPEPTR
                     FDDVDLEVRVAIPSGALEVRNNVARLRGSGDLTVRGTFGHPLLLGTIEAAEGGRLELR
                     GLRYDVTRAKLVFANPATNDPYFELEARTQVKEYAITLGVSGTASRIVPRFTSYPQLP
                     EAQIVSILATGEIPSTTAGSIGSVSPVSTDQDIVAAARELITGLATDAAASRTKEFLR
                     LDRLQIDPVFIGSTFDAPRLTVAKRLSNELTVTYSYKASTDQEQVLLVEYQLSPAAFL
                     QLLRDENGVYSAEMKIRQRLR"
     gene            complement(25425..26315)
                     /locus_tag="P5164_00635"
     CDS             complement(25425..26315)
                     /locus_tag="P5164_00635"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HRY42422.1"
                     /translation="MSTRPKARRRPVRRVGLVTRTTSGEAIHITRRLEAWLRKRGVEV
                     LHDAESMGARSAMGGLPRAEIGRHVDLLVTLGGDGTLLSVARHPAPGVPILGIDIGTF
                     GYLTACPPDAWEPLLADALAGTAPVETRRLLSVDVVEPGRPRRRIRVVNDAVIGKSAL
                     SRIATIRVEVDGRQVLRYRGDGVIVSTPTGSTAYNLSAGGPILAPTMPAIILTPICPH
                     TLSLRPMVLSDHVTLGLAVEGEPSDVYLTLDGQEGFPIGSSTRVRIARARETALLVRA
                     PGSTFFDVLAKKLSFGGEKG"
     gene            complement(26325..27086)
                     /locus_tag="P5164_00640"
     CDS             complement(26325..27086)
                     /locus_tag="P5164_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011251839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HRY42423.1"
                     /translation="MSPSKTRLDLLLVARGLAESRTRAEALVLSGRVRVKGVERPKPG
                     TPLPADAELAVEGPEHPWVSRGGVKLAGGLDAFGIDPAGRICLDVGASTGGFTDVLLS
                     RGALRVYAIDVGRGQLHAKLRSDPRVVLREGVNARLLSREHVPEPCSLLVADVSFISL
                     RLILPAAVPLLAPRAEALLLVKPQFESERGEVGRGGIVRDPAVRTRALERVVAAADAL
                     GLAHAGTVPSPITGADGNVELLAAFQRGAPPSGSD"
     gene            complement(27086..>27718)
                     /locus_tag="P5164_00645"
     CDS             complement(27086..>27718)
                     /locus_tag="P5164_00645"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRY42424.1"
                     /translation="LLPPHVSFTGGAFVEPVACALKAVDRALVRAGDAALVVGLGSNG
                     LLLGWLARLAGADLLVGSDPDPLRRRLALEHGFDAVVDPSAERLPEAVHAVSPRGADA
                     VFVVPTSPAAVLPAVEAAAPAGRVVFFSPVAPGETWPIAPNVPYFKDLTLRFSYSSGP
                     LETRRALDLIASGALPVERLVTHRLPLERTAEAFALAEGGGATLKVVVEI"
BASE COUNT         3693 a        10549 c        10025 g         3451 t
ORIGIN      
        1 cgccgacccc gagcccgcct accacggcct ctccttctcc gcgttcgccc ccgcggccta
       61 cgccgcccgc ctccggaccg tcctcctccg cgacgtcggc gccgcggtct cccccttcaa
      121 ctcgttcctc ttcctccagg ggctggagac gctcccgctc cggatccgcc gccacgcgga
      181 caacgccctc gccgtcgccc ggcacctcga gagccacccc gccgtcgcct gggtccgcta
      241 ccccggcctc gcctcgcacc cgacgcacgg gacggccgcg cgggtcctct ccggcggctt
      301 cggcggcgtc ctgaccttcg gcgtgaagcc gccggccggc gtctccgccg agcaggccgc
      361 gcgctccttc atcgcgaacc tctccctctt ctccctcgtc gcgaacgtcg gcgacgccag
      421 gagcctcgtc atccacccgt ggacgacgac gcacgagcag ctcaccgagg aggagcggct
      481 cgccgcgggc gtctcgcccg acctcgtccg cctctcggtc ggcatcgagg agcccgagga
      541 ccttctcgcg gacctcgacg ccgccctcgc gcgcctcggc gggacggagc gaacgcgatg
      601 agcgctcgca tcgagaccct cgtcgtgaag ttcggcggga cgtcgctcgg gacgcccgcc
      661 cggatccggc gcgccgcacg ccgcgtcgcc gcgctcgtcg actcgggccg gcgcgtcgtc
      721 gtggtcgtca gtgccatggg ccacacgacg gaccagatcc tcgagacgct cggcgccctc
      781 gaggccggcc gcaccgaggc cgccgcccgc gaggtcgacc gcgcgctcgc caccggcgag
      841 gacctctcgg ccgccctcct cgccgccgcg ctgaacacgc tcggcgtccc cgcgcggagc
      901 cttcgcggag gcgaggcggg cgtgagggcc gacggcgccc tcggcgcggc ccgcgtcgcg
      961 cgcgtcgacg ctcggcgcct ccgcgggctc ctcgcccgcg gggtcgtccc ggtcgtcagc
     1021 ggcttccagg gggagcgcga cgaccgcgag gtcgccacgc tcgggcgtgg cggctccgac
     1081 acgagcgccg tcgccatcgc ggcggcgctc ggcccggcgc cctgccacat cgtcacggac
     1141 gtcgacgccg tctacgaccg cgacccgcgc cgccacgtgg acgcccggcc gctcccgcgg
     1201 ctctcgcacg gcgagctcct cgccctcgcg cgctcgggcg cgcaggtcgt cgccgccgac
     1261 gcggcgcggc tcgcggccga gcagggcgtg ccgctccgcg tcctcgggta cgacgccgcg
     1321 ccgaacgacg aacgctcggg caccttcgtc gggggcgcgc ggtgagcggc ccccccgacg
     1381 tcgcggccct gagggtcggc ctcgccgagc accgggcggg cgacttcgcg ctcgagtcgg
     1441 acgagacgct ccgcgacgtg cggcaggcct tccgcctcga gggggagatc gacgccgacg
     1501 gcgggaacgt cgtcctcctc ttccactccc tcacaggctc cccggccgac ctcggcggct
     1561 ggcggccgtt cgtcggtccg gacctcccga tcgacacgcg ccgcttcgcc gtcctcgccc
     1621 cgaacctcct cggctcctgc tacgggacgc acttcctccg cggcgccggc cacgcgaaga
     1681 ggccgctcgc cgtcacgacg cgcgaccagg cgcgcctcgc cggcctcctc ctcgacgccc
     1741 tcggcgtgaa gcggctcgcc ctcgtcgccg gcggctcgct cggcgggatg gtgacgctcg
     1801 agctctgcgc gacgcgcccc gacgcggcgc gcgcggcgat cgtcttcgcc gcgcccgcct
     1861 cgccgaccgc ctggggcgcg gcctggaacc acgtccacag gacggcgctc gaggcggtgc
     1921 cccgctctgg cctcgccctc gcccggatgg tcgggatgct gacctacagg acgccgatcg
     1981 aggtcgagag gcgattccgg ccccacggtt ccgaaacgca ccccgtccgc gactacctgg
     2041 accaccacgg ggagaagctc ctccgccgct tttcgccccg tgcgtaccgg acgctcctcg
     2101 acgccatcga cgcgcacgac gtcgggcgcg gacggggcgg ggtgggcgcg gcgctccgcc
     2161 ctttccgcgg gcgcctcgtc ggagtcggga tcccgggcga cctcctctac ccgccggagg
     2221 aggtcctccg gtggacgcgc gccgcggggg ccgaataccg cgagctcgct tcgcggagcg
     2281 gacacgacgg cttcctcctc gagacgggag cggtcggcct gctcctcgcc gaggccctct
     2341 ctgttggcgg agcgggccgg tcgcccgcgc ccgcccctct cctcgctgct aatctggatg
     2401 ccggacctcc gccgtgatgc ccgcccgccg cgccgcccgg agccccgtcg cgtcgctcgt
     2461 cctcgcggcc ggcctcgccg tcgtcggctg cgggaagggc cggttcgcgc cgtcgccggt
     2521 cgcgctcccc gaaggaaagg tgtgggtcga ccacctctcg cgcgacctgg ccccgttctg
     2581 gctgaggccc gaggccctcg gatcgccggt cggggccttt ccgacattcc gctgcgacga
     2641 cgggagcctc gtcgatctcg agctcccgtg cggcgagttg ctcgtcgcgg ggacctgggt
     2701 ctcctcgcgc ctcggcgtcg agtacacgag gatgcgctcg cggcagacgt tcttctacgg
     2761 cgtcgccttc cacctgacgg gcgaggagcg ttacctcgcg ctcgcgaagg cgggcgtcga
     2821 ccacctccgc gcgcacgcgc tcgaccgggc ggagcggagc gccgtttcgt ggtggaagga
     2881 cggcgtcgcc gggccgcccg tcccggcgcg gagcgcgcag gacctcgcct acgcgaacct
     2941 cggcctcgcg ttctgggcct acctgacgcg cgacccggac gtcctcgccg acgcgctcct
     3001 cctctacgag gggatcttcg accgctacga cgacccggag cgaggcctcc ttcggtgggc
     3061 ggcgggaggc gtcggcggcg agaccgcgcg cgtcgagctc gtggcgcagc tcgaccaggt
     3121 gaacgcctac gccctcctcc tcgcctcgtt cgcccccgag ccgcaccgct cgcgcctgcg
     3181 tgccgacctc gcacggatcg cgagggtcct gatcgaccgc ttctggagcg cggagctcgg
     3241 cctcttccac ggctcgctcc acgaccccgt cgagcgcggc cccggcgccc gccacaccga
     3301 cttcggccac acggtcaagg cgctctggat gatcgagagg accggggcgc tcctcggcga
     3361 cgaggcgctc tcctcgtggg ctcgggaaag gctccccggc ctcctcgaaa aggcgtacct
     3421 gccgcgttac ggatgctggg ccacgggcct ccgcctcgac ggctccctcg acgccggcgt
     3481 cacctggtgg accgccgccg agctcgacca ggccgccgcc acgctcgcgc tcacggatag
     3541 tgggtacacc ggctacctcg agaagacctg gacctgctgg ctcgatcggc tggtcgacca
     3601 cgagaacggc gaggtctgga gctggatcga cctcgaccgc cccgagcgcc gaggcgccaa
     3661 gcagcacctc tggaagaacg gctaccactc ggccgagcac gcgctcgtct cgtacctcgt
     3721 cgcgcaggag ctgcacggcc ggcccgcgac cctctacttc gcgccggccg aggggacggc
     3781 gctcgggacg ttccgtccct acttcttctc gggagccgcc acggtcggcc cggagcggcg
     3841 cctcgagtcg atcccctccc ggcggtcgat cgccgtctcc ttccggggcc tccggtgaca
     3901 cgccccgcgg gatgcgaaca tcccgcggtg acctccgacg cgcagcgcct cgccgcccgg
     3961 atctccgacg ccacgctcgc cttccaggac gtcctgacga tctacctcgg ggagcgcctc
     4021 gggctgtacg acgacctcga gcggaacggc ccgtcgacct ccgcccagct cgcttcccgg
     4081 acggggactc acgagcgcta cgtgagggaa tggctcgagc agcaggcgtg tgcggggatc
     4141 ctgggctgcg cggagcccgg ggcgccggcc ggggagcgcc gcttcttcct cacgacggcc
     4201 tccgccgagg tgctgaccga tcgcgacagc ctcctctggt ccgccgcccg gccgcgcagc
     4261 ctcgtcgcgc tcgcgcagaa gctcccgatg ctgcgggagg cgttccggag cggcagggga
     4321 atcaccgacg gaatggaaga cgcccgaacg gcgcagggcg acctggggcg cgccgcgttc
     4381 cttcggcagc tcgcgacgga gtggctcccc gcggttcccg acgtcgacgc gcggctccgg
     4441 gccgagcctc cagcacgcgt cctggacgtc ggctgcggct cgggctgggc gtcgatcgcg
     4501 ctcgcgcgcg gctacccgct cgcgcgggtc gacggtctcg acctcgaccc ggccgccgtc
     4561 gaggacgccc gagcgaacgc gcggacgcat ggagtggagg accgcgtcgc gtttcacgcc
     4621 tgcgacgccg cggcgccgga gctccgggag ggatacgacc tcgtgatcgc tttcgaggcg
     4681 atccacgaca tgccgcgccc ggtggaggcg ctcgccgcga tgcgccggct cgcttcgccg
     4741 tccgggaccg tcctcgtcgt cgagatgaag gtcggcgaga gcttcgccct gccgcccccc
     4801 ccgaacgacc gtctgaacta cggctggagc gtcctgacgt gcctctcgtc ctccatgtgc
     4861 ggcgaggagc ccgcggggac cggaacgctc ctccgccgcg ggacgctcga gagctacgcg
     4921 cggcgcgcgg gcttctccgc gatcgaggac gcgggcgtcc cgcacgaggg atggacgttc
     4981 tggcgcctgc ttggctgagc ggggcggaac gccgaccgcg cgtcctacac tctccggcgg
     5041 gagggagcgg agcttgaaga ccgagaggac cggccccgcg gcgttcacgg tcgagttcga
     5101 gtccgaggcc gagctcgaag ccgaggtgcg gacgaacctc tccgcgggcg gactgaggct
     5161 cccgacgcgc gagaagctcg cgatcttcgc gaagctccag ctcctcctca ggctcgcggg
     5221 gggcggcgcg gtccccgcga cggcgaccgt cgtcgcgccc cttcccgacg gcttcgccct
     5281 tcacgtcgaa ggagacgccg ccgtcctcgc cgacgcgctt cgcgacgcgc ccgaggagcc
     5341 cggggaggag gtcctcgaag aggccgggga cgcggaggac ccggtcgccc gggagagcgt
     5401 ctgggaccgg ctccgcggcc tgacgcgcgt cgagaagctc ctcctcgccg cgaaggccga
     5461 tcgggtcgag cggatgatcc tcgcgcagga cagcgacccg caggtcctct actccctcct
     5521 gaagaacccg cgtctgacgg ccgacgaggt gatccggatc gccaagtcgc cgttcctgac
     5581 gttccagacg gccgaggcga tgatgaagtc gacgcagtgg ttctcgaacc tcgaggtgcg
     5641 cgtcgcgctc gtccacaacg cgaagacgcc ccccgccttc gccctccgga tcctgccgac
     5701 gctccccgag tcggaggtgc ggaacatctc gcgcggcgcg gcgacgagca tggctctgaa
     5761 ggtcgcggcg ctgaagaagc tccagggctt cacctgagcg gccgccctcg tcgtgggtcc
     5821 atactgcggc gggccggacg gccgccacat cccggcccga ggaggaaacg atgcccgagc
     5881 ccgcgatccc gtccctgtcc gaggcggccg ccgcgctcgg cctgccgccg gaacgcctcg
     5941 tcccgtacgg ccacgacaag gcgaaggtgc tcccgtctcc cgggggcttc acgccgcggg
     6001 gccggctcgt cctcgtctcg gcgatcaccc cgacgggcgc cggcgaaggg aagacgacga
     6061 cttccatcgg cctcgtccag ggcctcgccc ggatcggcgc gcgcgcctgc gtcgcgctcc
     6121 gcgagccctc cctcgggccg acgttcggta tgaagggggg cgcgacggga ggaggccggg
     6181 cgcgcctcgt cccgtcggac gacatcaacc tccacttcac cggcgacttc cacgccgtca
     6241 cctccgcgca caacctcctc gcggcgctcc tcgacaacca cgtccaccac ggcaaccggc
     6301 tcgacatcga cccgcggcgc gtcttctggc gccgcgtcct cgacatgaac gaccgggcgc
     6361 tccgcgacgt cgtcatcggc ctcgggggcg tcctgcaggg ggtcccgagg gagaccggct
     6421 tcgacatcac gcccgcctcc gaggtgatgg ccgcgctctg cctcgccgaa gacgccgagg
     6481 acctccgggc acgcctctcg cggatcgtcg tcgcgctgac gttcgggaag gagccggtca
     6541 cggcggccga cctcggcgcg acgggcgcca tgctcgcgct gctgcgggac gcgctccttc
     6601 cgaacctcgt ccggacgggc gagggggtgc cggcgttcgt ccacggcggc ccgttcgcga
     6661 acatcgccca cggctgcagc tcggtgatcg cgacgaagac ggcgctctcc ctggccgact
     6721 gggtcgtcac cgaggccggc ttcggcttcg acctcggggc ggagaagttc ttcgacatca
     6781 agtgcgccgg cgccggcctc gacacggccg ccgtcgtcct cgtcgcgacc gttcgggccc
     6841 tgaagcgcca cgggggcgtc ccgaaggagg ggcttacgaa gcccgatccg ggcgccgtcg
     6901 agcggggcct cccgaacctc gcgaagcacc tcgagagcat ccgcgcgttc ggggaggtgc
     6961 cggtggtcgc gctgaaccgg ttcacgaccg actccgagga ggaggtctcc gtcgtccgcc
     7021 gcgccgcgga gggctggggc gcgccgttcg ccgtctgcga cgcgttcgag aagggaggcg
     7081 agggggcggt cgcgctcgcc gaggcggttc tctcccacgc ggagcggaag tcgagcctgt
     7141 tccggccgct ctacgcgcgc gaggactcgc cgaaggagaa gctggcgaag atcgcccgac
     7201 acatgtacgg cgcagcgtcc gtcgagtgga gccgggaggc ggagcgcgac ctcgcggaga
     7261 tcgcccgcct cggatacgag aagctgccgc tttgcgtcgc gaagacccag atgtcgctct
     7321 cggacgaccc cggcgtcgtc ggccgcccgg aggggttcga cgtgacggtg cgaggcgtcc
     7381 tcctcgcggc cggcgccggc tacctcgttc ccctcctggg ggacatcctg cgaatgccgg
     7441 ggctcccctc cgcgccgcag gccgagcgga tcgacctcgt cggcgggagg atcgtcggga
     7501 tcagctgacg gggggcccgc cccctcgtcc ttccacgaac gcgacgagct ccttcagcgg
     7561 gagcggcttc gcgtagagga atccctggaa atggcggacc ccgcgctcga cgaggtagtc
     7621 gacctgctcc tcccgctcga cgccctccgc cacgaccgtc atctcgagct tccgccccag
     7681 ctcgacgagc gtgtcgacga ccgtctcggc gaccgagccg gtgccgatcc cctcgacgaa
     7741 gctcttgtcg atcttcaggt ggtcgagccg gaagcggcgg agcgtggcga tgttcgagta
     7801 gccggtgccg aagtcgtcga gagcgatcgt cgcgccccac ccctggagcg cctcgaggac
     7861 gttacgggcc gtcccgttcg cgtcgtcgat cagctcccgt tccgtcacct cgaagacgag
     7921 gctcttcggc ccgagccgcg agcccgcgaa cgcgcggagg acggcggcca cggtcccggg
     7981 atcgcgcagg tgggccccgg cgaggttgat cgccacgtag aagcggtcgg gctcgcgcag
     8041 cccgtcgggc aggtcccgcg cgacccggcg catgagccac tccgtgatcg ggacgatgag
     8101 gcccgtctcc tccgcgaggg cgatgaagag gtcgggcgag aggaggccct cgcgggcgtg
     8161 acgccaccga aggagggcct cggcgccgac gcatcggccc gacgtgacct ccacgatcgg
     8221 aaggtaccgg acctcgagct cgccccgccg gatcgcaccc cggagctgcc gcgtcagcgc
     8281 cgcgccgggc gagcccgcga ggaggacgag cgcggcggcg ccggcgccga acccgagtcc
     8341 gatcgccacg aggagcggga gcgtcgcccc ccacgcggcg agctgcgcct cgcgcgaggc
     8401 gcgcaggagg acggaggcgc cgccccccgc acaaggcgcg gcggccgttt cgtcgcgcgc
     8461 gccgacgaga gggccgagaa ggcgaagcgg cagggcgagt cttcgaggct cccgagagcc
     8521 cggcagggcg aggacggggc cggcgacgcc ccggacctcc accgcgtccc gcccgcctcc
     8581 cgcgagctcc gagagtgcgc gcgcgatcgg cccggcgtcg aggctggcct cggccgcacc
     8641 aaacggaagc cggagcgcga ggcgcaggag gtcccccggc gctccccctt cccagacggc
     8701 gcgcgcgtcc ccctcgagcg cggtccggac ggacggcggc agcggcgccg gcgccgttcc
     8761 cgccgagcag gccgggtgaa gctctgcgtc gaggagggcc acaccgcgaa cgccgctgcc
     8821 gagctcgagc gccgcccgga gagcctccgc ggtctccggc gcgcagctcc cgtcccagcg
     8881 gctgccgagc cgtgtgagga gcgactccgc gcccgcgagg agggcgtcgg tcagcgcggc
     8941 cgccgaccgg gcccgccgtt cgaggtcgtc cctcaaagcc cggctctcga gccggaacga
     9001 gagccagacc agaagcgccg gcggcaggag gaccgccagc aggaccgcgg cgacgagccg
     9061 caacggccag cgcgtcacgg cgctcccgcg aggacacgcg ttcgtccgcg cttcatcggt
     9121 ccgtcgattc taaggagcgt cccgcctcct tcatcggaga tagccgagcg accggaggct
     9181 ctcgacgtac tccttcgggt cgatcggcgc cgcgcctccg cgcggccgtc cgccccagga
     9241 cgcgaccgtc gccgaagtgg cggcgcccgc gcggacgagc cgggagtcga cgcgcccctc
     9301 cgtctggcga gaggcgggga cgccgagcgc ggcgaggagc gtcggcgcaa ggtcgacggg
     9361 ccggaacccg cccgacgctc cttcggccgc cagcggcccg gcgaccagga gctctccgtc
     9421 ccgccccgag cggccgggat cgaggacgag ggcgacgatc tccgcggagg agccgaggac
     9481 gtcggggccg gcgaggaagg aggcgacgtc tcgcgcctcg tcggcgagcg cggccgccag
     9541 cgcggcgcgt tcgccgacgc tgcgccccgg ctcggagagc gccgctccga gaacgtcgag
     9601 gcccgggaga tagaggaggg agagacgcgg ccgggaccgg gccgcctcct cccggaacgc
     9661 ggcgatcgcg aatgcgtcga gcccccgggc gtcgtcgacg agacgcgcca cgccgtcggc
     9721 ccccggggca gcccggggcg ctgccgccgc cgcccgcgcc gccgcctccg ccggccagcc
     9781 ttcgccttcg agcggccgtc cggcgcgcgc ggcgaagaag aggtggttcc ccaggacgac
     9841 tcctcccgcg tctccggcgg gccaggtcgt ccaccagttg accgtcagcg acgcgaggcc
     9901 gccccgatgg gccacctccc agatcgccgg gacccttcgc gcggccgagg tgaccgactc
     9961 ctcgcgggcg gcgccgagcg ccggcaggag ccgtccgagg taccacccgg tcccggccgt
    10021 cagcttcacg ggcgcggtga ccccgtccag gcggacgagg tcgagggccc ccacgccgtg
    10081 ccgtcgcgcc ggctccccgg tcgcgaccgt cgtccagaac gccgccgggt ccgccgtctc
    10141 cttgcggtag gagaatcgcg acgcggcggc gaggcgcgcc gcgtccccgg cgagctcggg
    10201 gagccagccg tccacggcca cgagcacgac ccgcgccgtc cccgcacgga cgacgagcgg
    10261 ctcggccggg ggctccggac gtctcgcgag gagccatgcg cccgtggcga cggcgatcgc
    10321 cgcgagcgcc gtcccgagcg cgagcgcggg ccgcggcgcc ctcgagagga ccgagcgccc
    10381 cgatgccgcg aggacggtga acgcgaggcc gcgtgcgacg cgaacggcga ggacgacggc
    10441 cagggccgcg gcgccgaacg ccacggccgc cgccacggga ccggccgcgt ccaggacggg
    10501 gcgcgcggcg agggcggcgc cgacgcccgc gaggaggccg aagccgaccg cgacctccgt
    10561 cgaggcgcgg gaagggtcgc tccgcccgcg ggtccggagc cacgcgacgg ctccgagcgc
    10621 gaggacgagg cccgaggccg cgacgagggc cccggcgccc agccaggcga agaggacgag
    10681 cgcgtcgcgc ggagagagcg cggaggcgag cgcgacggag gccgtctcga cgacggcgag
    10741 cgcgagggcg aggaggaggg aggcgagggc cgctccgcgc aggagggctc gcgtgcgcgt
    10801 ccgcgcggcg agcgccccct tcaggaccgc cccttcgacc ccccggtcca ggtacccgcg
    10861 ggcccggaga cgctcgcgcg cctcctcgaa cgtcgcggga tcgcgctcgg cgggggcctc
    10921 gtcgacggga tcggtcacgg cgcggacctt agcagggcgc gggccgcgac gcccgccctc
    10981 aggccgtccg cgcgccgagg agcgacgcga actcgcggag gagcgccccc ctcgcgccga
    11041 acggctcgag ggcctccacg gcgtgcgccg tcagctccgt cacgaggcgc cgggccccct
    11101 cctcgccgac gtgccgcgcg aaggtcggag ccggggcgcg cttcgcgtgc ttccccgtcc
    11161 gcgccgggtc ctgccgctcc agcaggtcgt cgacgatctg gaaggcgagg ccgacgttcc
    11221 gcgcgtagga gcggaccgcg gcgagctcgc gctcgcgggc cccgccgagg accgcaccga
    11281 actcggccga cgccacgaag agcgccccgg tcttccgggc gtggatcgtc tcgagccggt
    11341 cgaaggaggc cgccgcctcg ggcgtccgga ggtcgagcga ctgtcccgag gcgaggccga
    11401 ggaggcccgt cgcgtcggcg aggcgggaga cgaggtcgag cgccgtcgcc ggccggagcg
    11461 cggagagccc cgcgtcggcc aggacgccga acgcccggac gatgagcgtg accgcggcga
    11521 ggatcgcgag gtcctcgccg tggacggcgt ggagcgccgg gcgtccccga cgcgtcgtgg
    11581 cgtcgtccat cgacgggagg tcgtcgagcg cgagcgatgc ggcgtggatc atctccacgg
    11641 cgcaggcggg gtcgagaagc ctctgctccg gcgcgccgaa gaggtcgccg acgaggaaga
    11701 cgaggcccgg ccggactctc ttcccgggcg agaggagcgc ctcccgcgcc gctccgggga
    11761 gcgcgtctcc gacggccgct gcgtccggtc cggaggccgg gtccgcgagg aggcccggga
    11821 gccgatcgtc gacgacgcgg cggacgcggg cgagggtctc cggaagggag gtgggcacgg
    11881 cgggcggctc cggcttcgat cgacgcgggc gattcggagg agcggaaagt gtagcatcgg
    11941 cgcgatgcca gccgcgtcga cgaagacgtc gtcccgcgcg agggaatggc tctcggccct
    12001 cctcgcgccg gcggtcgtcc tgctcgggtt cgccgctctc gccctcgccc gcggtccttc
    12061 gcccctccgc atcctcacga cgggcctcct gacggccgcg gccgtcgccg ccctcggacg
    12121 gctcgcgtcc gggcgtctcc tgcgctcgcg ggaggcgacg cgggacgcgc tgcaccgcgc
    12181 ctcggccggc gacctcacgc tctcgcgggc ggcgctcgcg cggtccggag agaacgccct
    12241 cgccgaggcg ctccacggcc tgatggtcgc gatggagagg atcctggcgt cgttcgcgcg
    12301 cctctccgcg gccgtctccg gcgtcgccgc ggacctcacg acccggggac gcgagctctc
    12361 cctcgccgcg gcgatgcaga gctcgcgcgc ggaggagacc gccgacgcga tgcgcggtac
    12421 cgacgcggcc atcggctccc tgcgcgagtc gatggagacg ctcgccggcg cggccgagaa
    12481 cgccggcgcc tcgctccacg agatgacggc ctccgtcacc caggtctcgc ggagcaccac
    12541 ggcgctccgc gcgttcgtcg acgagacgac cgcctcgctc ggcggcgtcg tctccgcgct
    12601 ccagcaggtc gcctcggccg tcgagaacct ctcgggcctc gcggacgaga ccgcccgcgc
    12661 caccgccgcg atccgctcct ccaccgccga gaccgaccgg caggcccaga ccggtgcgcg
    12721 gctctccgag cgcgtctcgg cggccgtcgt ctcggggaag accgccgtct cggggacgct
    12781 cgccgggatg cacgagatcc gggcggccgt ctcgggcgcc gccgacgcct ccacggcgct
    12841 cgccgagcac agcgcgcgca tcggggagat cctccacgcg atcgaggaga tcgccggcga
    12901 gacgaacctc ctcgccctca acgcctcgat catcgccgcc caggccggcg agagcggcaa
    12961 gaccttctcc gtcctcgccg acgacatccg cgacctctcc gaacggaccg ccgtctccac
    13021 cgaggaggtc cgcggcctgg tctcttccgt ccgcggcggc gtcgaggacg tccgccggct
    13081 cctctcggac gcccggcgac ggacggagga aggcgtcgac ctcgcacaga cctccgacgc
    13141 gacgctcgac gagatcgagg cgctcgcgct cgagtcccgc cgcgcgtcgg aggggatcgc
    13201 cggcagggcg gtggcgcaga gcgccgacgc cgcccgcgtc tccgaggcga cgggccgggt
    13261 ctcggccgag gtcgtccgga tctccgaggc gacgcgcgga cagctcgaga cgacgcggac
    13321 cgtccacgct cgggcggacc gcgtccgcga gctcacggag cagctcgcgc gggcgatgga
    13381 ggagcaggcc gtcggcggcc agacgctcct cggctcgatg gagcgcgtca cctcgaccgt
    13441 cgacgccatc gcggaggcga ccgtgacgct cgccgacggc tcctccgccg tcgtcgggtc
    13501 gatggacgag atccggaggg ccgcgcgcca gaacgcctac gccgcgaccg cgatgaacca
    13561 ggcggcccag gcgctcgagc aggagtcgct cctcctccgc tcccgctcct cgctcttccg
    13621 cctcccgtcg cccgcgccgg gcgggcggat ccgcgccgct ctgcgctacc tcgacgaggc
    13681 gaacttcgac cccgccttca gccagaccgt tccccaggcg atcctcgcgc ggacctgggg
    13741 cgaggggctc gtcaagttcg gcgaggggac gcggatcgtt cccgagctcg ccgagcgctg
    13801 ggagatcgac ccgaccggga cgctcttcac gttccacctc cggcgcggcg tgaggtggca
    13861 cgagggcggt actctctcgg ccgccgacgt caaggcctcc ttcgagcgct tcctctcgcc
    13921 ggccaccggc gcgccgctcg ccgccctctt cgacgccgtc gaggggtacg aggagttccg
    13981 ggccgggcgg agaccgggcg tcgccgggct cgagacgcct tccggctcgg tcctgaacgt
    14041 ccgcctctcg cggcccgttc ccttcttcct gcagctcctg accctccccg acatcaccgt
    14101 cctgccgccg gcgatcctcg gcgacccgga ccggatccgc cgcttcccct gcgggaccgg
    14161 cgccttcgtg ccgcgggaga tcgcgttcgg gaagtccgcg cgattcgacc gcttcgacgg
    14221 ctactgggag cggaaccgga tcgcgcccga cggaatcgac ctcgacctcg cggaggactc
    14281 cgaggcgggc gtcttccagc ggttcctcga cggccagctc gacgtcgtct gggacatccc
    14341 gtaccccgag gccgcccgcc tgatggccga tccggcgtgg cggccgtaca tcgactcctc
    14401 ggtccagctc cacacgtcgt tcctcgtcct ccggtgcgac cgggcgccgc tcgacgacgt
    14461 ccgggtccgc agggcgctca accatgccgt cgaccgggcc gcgctcaacg cgaggacctt
    14521 cgcgggcctc tccgtcccgg ccgcgtccat cctccctccg cacgtcgtcg ggcacgaccc
    14581 cgccctccgt ccctaccgcc acgagccgga gaccgcgaag gcgctcctgc gcgaggcggg
    14641 ccacggggac gggctcacgc tctccgcctg gctcagcccg aaggacgcgc gcgacccgct
    14701 caacccgctg ccggtcatcg catcccagct cgaggcggtc ggcgtgaggc tcgcgctcga
    14761 gatcctcccc ggcgaggagg cggcggcgcg caagcggaac ggcgtcttcc cggacgtgag
    14821 gctggcgcgc tggttcgccg acttccccga cccggacacg ttcttccgct ccctcttctc
    14881 gtcgaaggcc gagagcgtcc tcgacggcgg ctacgccaac gccgaggtcg accggctcgt
    14941 ggagaagggc gccggcctcc tcgacgcggc cgagcgcgag gcgctctacc gcgagctgga
    15001 ccggctcgtc cagtccgagg cgccgctcgt cttcctcttc cacaatcgcg gcttcgtcgc
    15061 gcacgcaccc cgcctccgcg gggtccaggc ccacctcctc ctgccgccgg tgaggtggaa
    15121 cgagctctgg ctcgagccct gacacggccc cacgcgcgga ccgaagacgg aacggcccgg
    15181 cgcgccccga ggggcgggcc gggccggcgg atcgggcgtt cgacgcgaac gccgaggctc
    15241 aggcgctgaa gctcgcgatc gcttccgcct cgttcgggta gacgtcgaag accgtgatca
    15301 gctgcgtcac cgtgaggacg tcgctgatct tggccggcag gttcatcagc ttcatcttcg
    15361 cgcccttgtg cgtggccgtc gtgaagcacc cgacgagctc gccgacgccg gaggagtcga
    15421 tcgtgacgac ctgcgacatg tcgacgacga gcttgcgggc cccttccgcg agggcggcgt
    15481 gaatcgcctc cctcatctgg aggtcgccgg cgccgatcgt gatcttcccc ttcagctcga
    15541 tgatcgtgac ttcgcccgcc ttgcggatgc tggcgttctt gttcatggct tctctccctt
    15601 cgcgtctccg cgtttgctga ggtccttgcg catcgtcagg acggttccgc ccccctcgcc
    15661 gcgcgagaag gtgacgtcgt ccatgaacgt ccgaatgtag aagatccccc gccccgaggt
    15721 cttcagctgg ttctcggggg cgagcgggtc cgagatccgt tccgggtcga agcctccacc
    15781 ctcgtccccg atcacgatcg tcaggtcgtc gcccgccgat tcgagcctca cgaggacgcg
    15841 cttgtccggg tcgagccggt tgccgtgctt caccgcgttg gcgaccgcct cccgcatcgc
    15901 catcccgatc cagtgctcca tctccgcgtc gatcccccgg aggcggcaga cgtgggcgag
    15961 cgccgcctga gccagctcga tgttctcgta gcggctcgaa agccagagga cgatggggcc
    16021 gggcgctgct gcgggcatcg tttcggtctg tcaggacgcc gcaccgggac cgggagcgac
    16081 ggggtcaggg gccccgcccc tgctaagctg cggcgccatg tgcgcgcccg agccctgcga
    16141 cgacgtcgcc gaaatcatac gcgactccgg tcgacggcgt ccactctcga ggccctcact
    16201 ctccgtcgtc ggcgtcctcg ggctcgccgc tctcgccacc gtcaccgcgc cgcccgcccg
    16261 ctccgacgag tcggccctcc tcgccgtccg ggtcgcgaag gcccagaagc tcgtcgagaa
    16321 ggtgcggggc gtctccttcc gggccccggt cgcctccgcc cttcttccgg agagggagct
    16381 cgagacggtc ctcgcgaaga agctcgtcca ggacctcccc gtcccgttcg acgactacgc
    16441 cgccggcctg gccgccctcg gcctgatcga gccctctccc gggctcctcg agaagctcac
    16501 ccgcctctac acccgccagg tcgtcggctt ctacgacccc gcggagaagc gcttctacat
    16561 cgttcccgag aggtcccggg acgcgggcgg ccccgcgggc gacctgatgg agcagctcct
    16621 cctcgctcac gagctgaccc acgccctgca ggaccagcgc ctcggcctcg accggaggat
    16681 gaagacgctg cgcgactcga cggattccct gctcgccctc caggccttcc tcgaggggga
    16741 ggcgacggtt ctgatgacgg aggcgctgct cgagagcgtg cccgaggagg cccgtgaggc
    16801 cctcggagcc gacccgctcg gacaggtcct cgagggcctc gacgacccgg acggcgtcga
    16861 aggagcggac ggggtccccg cgtatttcgt ccgggagctc gtcttcccgt acgccgccgg
    16921 caccgcctgg gtgaggcaga agaaatcggc cggcggctgg cgcgccgtcg acaccgcgta
    16981 ccgtcgtctc cccacgacga cccgggagat cctccggccc ggcgtcgccc tctctccccg
    17041 cctccgcctg gccccggccg accggccgac gccgggcctg gtcccgggcg gcgggaacgc
    17101 gtcctgggcc gacacgctcg gcgagtgggt cctcgggacc ctcctcgagc aggccggcgc
    17161 cggcgacgcg tcccgcgacg ccgccgcggc gtggcaggac gaccggatcg tcttctcgta
    17221 ccccgtcgcc tccccgggcg cgcatcgcgt cggcttcctc tggcgaatcc gggcggcgag
    17281 cccgcaggcc gcggcccgga tcgccgagct tctcgagccg atctacgcgg cgcgacccgc
    17341 tcccgcccgg ccccgggtct ccgtccgcgg cgacgtcgtc gaggtcgcct gcccggccgt
    17401 tccgccgccg cccgcctgag cctcagcccg ggatcgcccg gaccgcggag gggaggacca
    17461 gaaggagctg gaccgcgtcg aacgtgccgt gggccaccat cggcgcgagg acggagcccg
    17521 tcttctcgaa cgtcgcggcg aggatcgcgg tgatggcgag gaggccgacg aagtagagcg
    17581 gatcgccgta gcccccgtgc gagacgagga agaggatcga ggcggggacc gcgccgagcc
    17641 ggcgctggag gaagctccgg aagaagacct cctcgacggt catcgccgcg aggatgagga
    17701 cgaggcggct cgcgagcggc tggctcgcga gccagcggac gagcggcggg atcggccgca
    17761 cctcgtccat gccggcgaag cggaggacgc ccaggacgac gagccccgcc gcgaccgtca
    17821 gcccccaccc ggcgaggccg acgacgagcc ccgtccgcac ctcccgcctc gggtccggcg
    17881 ccgtgagcgc gaggaactcc gcggcctccg gccgccccgc gaggagccac cacccgatca
    17941 ggaagacgag caggagctgc tggagcgcga ggatcgacgg aaggctcagg cgggccgggt
    18001 cgatcgggcg cccgagggag aacgccggca ggatcgtcgt ggccgcgagc gtcacgccga
    18061 gcgcgacgag cgcgccggcc cgccgcctct cgtgggggaa ctcgtccctt cggaatgcgc
    18121 cgcgacgcgc cgcgacgacg acgatcgcgg cgacgaccgg caggccgacg ccgaggagga
    18181 ggaagaggtc gatcggcttc acggccgcct caccgggcct cttccgggac cggaggggga
    18241 ttcgtgcgcg acgtcgaagg gctcgggtga gcgttcatcc cgcgggcggc gaagtctagc
    18301 agcgcaccgt gaagggagac ctccgcgagg gcgagccggt ccggcgacgc accccggggg
    18361 cggtgaagcg gcccccgaac gaaaaaacgg cggaccttcc cgccgttcct tcactcgtct
    18421 tcgtcttctc tcgaaatggc ggggccgacg gggctcgaac ccgcgacctc aggcgtgaca
    18481 ggcctgcgtt ctaaccaact gaactacgac cccgtttcga aagagcggcc cttgcgggcg
    18541 gacgcgcatt atacgcagga gcctctccgg cgcaagcggg gccccttcgc cgccgctttc
    18601 gctccgtcag aacggatacc cgatgctgaa gctgagcgcg cccgtgtcct cgtccttctt
    18661 ccggtcgaga cggaagccgt attcgagacg gaaggggccg accggcgtca ggtagtggat
    18721 cccgaggccg gcgccccagc gcccctccgg gagcttcacg taggcggggc cggcccagac
    18781 gttcccccag tcggcgaaga ggacgacccc gagcccgccc gtcagcggga cccgccattc
    18841 cacgttcgcc aggaggaacg cgttccctcc gatgccccgt ccgcccttga gcgtctggcc
    18901 ctccaccccg agatcgtcga gcgggaagga acggtgggtc gtccggcctc cggcgaagac
    18961 cctttcgacg atcggcgtgt cgaggttcgg aaggcatccg gggttcgcct ccgggttgca
    19021 cgtgacgagc gacctggccg ctccggcccg gacgccggcg gcaagcgtcc ccccgcggaa
    19081 cggccggtag agagagagct gcgtcgagcc cttcaggaaa tgggcgtccg cggcggcggc
    19141 cgggaaggcc cacttcccct ccgccgccgc gaagaacccc ttcgacggga cgatcgggtc
    19201 gtcccgccgg tcgacgacgg cgctcgcccc gacggagttg atcaggatcc gctggtcctg
    19261 ccgctcgatc tccgagagga tgtcgggcgg cgcgtcgggc tcgacgatct cgtactggta
    19321 gcgaagcgcc cccttcaccg tcccggcgaa gacccggctc gcctcgaaga aggcgcctcc
    19381 gcgccgaacg ccgtacgact gccggatctc ctcggcgacg tagccggtga tcgtgcccgc
    19441 gagcccggag tcgaagaccg atcggtcggc gaggcggagg aggacgcgcg tgtcccgcct
    19501 cgagtagcgc gcctcgacga cgcctgcgat cgcgcgcccg aagaggttcg catgcgtcag
    19561 ggcgagcgag aggcgggggt tgaaggcctt ctcggcccgc tcgtcgtact cgacgccgag
    19621 cccgtagagg aggctccacg gcttcgtctc ctccacctgg acgaggatcg agcgcgtgga
    19681 gccgcctggc tcgggggaga ggccggagac gtcgacgcgc gagaagacgc ccagccgatc
    19741 gaggttctgc tgggtccgga tgagccgtgc catcgagaac ggagccccct cctcgtaggc
    19801 gagctcgcgc tcgagcacgg agaggcgcgt cgccctcgct ccccggagga tggtcttccc
    19861 gaagacggcc ctcggcccct cggagacctc gtagacgacg tcgacgacga cgggcgcccc
    19921 cggctccgcg gcgcgccgcg cggccctcgc ctcgacgcgg ccgtccggga agccgccgtc
    19981 ccccagcgcc gaacggagcg ccgtgaggtc ggcgtcgacg tcctcctgga ggaaacgacc
    20041 gccgggccgg ctccgaaggc gccgcgagat cgcggcgacg tcgagcgtgg cgagcccgtc
    20101 gaccgagacg cggccgatcc aggcggccgg cccctcgtcc acgcggaagc ggacgtcgag
    20161 ctcgtacggg agcgccccgg cgaagacctc ggcggtcacc ttcgcggccg ggaagccgcg
    20221 cgcgcgatag acggaggcga tcgctgcgcg gtcggccgcg aggtccgggt cgacgagacg
    20281 ccccttctcg aagagcccgc gcgggcgggt ccgcaggacg gcgaggagcg ttcgctcggg
    20341 gatcgcgcac accccttcga cgaaggtgcg gcccacgacc cagcgctctc cccgctccgc
    20401 gtggaggcgg acttcctcgc ggtccgccga cacctccacg tcgacgtcga cctcggcgcg
    20461 cgcgtagccc ttcccctgga gctcctcccg gagcgcgctg cggagccggg cgagggcatc
    20521 cgcgtcgggc gggtccccct tcgcccaggg cgaggccggg tgcctccgga cgacggcctc
    20581 cgcgatcccc gtgacgtcga ggacgacgtc ggggccggcg aagacggcgt accgggcggt
    20641 cgcccgtccc gacccccggt cgtacgaaac gccctcgtag cgcacctccg cgcgggaccg
    20701 gcccagctcg cggtaaagcg ccgcgtagcg ctcggcgtct tccctcgcga ccccctcccg
    20761 gaagaccccc tccctccgga gctttccctt cctccggagg tcgtcggccg cgagaggccc
    20821 gaggtctccc gtgaactcgg ccggggccgg cgccgccggc cggcccgcca cgacctcgaa
    20881 gcggacgttc accgaggtct cgtccggccc gggcgagacc cacggcgtca cctcggcctc
    20941 gaagtagccg cgctcctcga ggacgcggag cgccgagcgc gtcgccgtcc ggccgcgctc
    21001 ctcgctccac gcgtcgcgcg gacgggtctt cagcgcgtcg aggacgcgcc ccttcaccgg
    21061 gatccgctcc cccgccacct ccacctcggc gacgcgtgcc gcggcggaga agacgatcgt
    21121 gacggcggct ccctccgcag ccggcgccgc ctccacggcg aggtcggcga agcgcccggt
    21181 ggcgaagagg ttccgcagcg aggagtggac ggcgccctcc gtcagcagct gcccggggcc
    21241 gagatcggtg aggcgcgcga acgacgcttc ctcgatcgga gcgtctcccc ggaagccgag
    21301 cgactcgatg cgccgccccc agagctcctc cccgcgcgcc ggccaggcgg cgaggacgag
    21361 gagcgcgacg agaaggaagc gcgccctcaa cggagcctct gccggatctt catctccgcc
    21421 gagtagacgc cgttctcgtc ccggaggagc tgcaggaaag ccgccggcga gagctggtac
    21481 tccacgagga ggacctgctc ctgatcggtg gaggccttgt aggagtaggt gaccgtcagt
    21541 tcgttcgaga gcctcttggc gaccgtgagg cgcggcgcgt cgaacgtcga gccgatgaag
    21601 acggggtcga tctggaggcg atcgaggcgg aggaactcct tcgtccggct cgcggcggcg
    21661 tcggtggcga ggcccgtgat cagctcgcgg gcggccgcca cgatgtcctg gtcggtcgac
    21721 acgggagaga cggagccgat gctcccggcc gtcgtgctcg ggatctcgcc cgtcgcgagg
    21781 atcgagacga tctgcgcctc gggcagctgc ggatacgacg tgaagcgcgg gacgatccgg
    21841 gacgccgtcc cggagacgcc gagcgtgatc gcgtactcct tcacctgcgt tcgcgcctcg
    21901 agctcgaagt aggggtcgtt cgtcgcggga ttcgcgaaga cgagcttggc tcgcgtgacg
    21961 tcgtagcgca gaccgcggag ctcgaggcgg cctccctccg cggcctcgat cgtgccgagg
    22021 aggagcgggt gcccgaacgt gccgcggacg gtcaggtcgc cggagccgcg caggcgcgcc
    22081 acgttgttgc ggacctcgag ggccccggaa gggatcgcga cacgcacctc gaggtcgacg
    22141 tcgtcgaacc gggtcggctc gggaggcgcc tccgcggcgc cgcgccgtcc tccgaggagc
    22201 gcctggaggg agaggtcgag gtcctcggcg tagaggcccc gcgtgagcgt cagctcgccg
    22261 cgggccgagc ggagctcgtc gtcgccgagg aggacgaggt cgcccgagac ggtcgagcga
    22321 aggccgggga acggctcgtt gcggagcccg tcgaggtgga cgttcagcct catcccggtc
    22381 cacgcgaggc cgtcgagggt cgcggcgcct gcgaccgcca cggctccgtt ccagcggagc
    22441 gtcaggtcgt cggtcgagac ccgacccggc gtcagaagga tcgtgcccga gatcgcttcc
    22501 accggcacct cgcccggcga cgtgacgagc tcgaggccgt cgagcacgac ccgcccctcg
    22561 aagacgggcc gcgccgtcgt gccggaagcc gtcgccgaca ccgccatgcg gccttcgagg
    22621 ctcgccacgc cgaggaacgg cgcgaggaag ccggcctcca tcgtcccggt ggcccgcacg
    22681 tcgatcgcgc ccgcgccgcc ggtctcgacg ctcccgtcga tcgtgacctc gccctccggg
    22741 agcgccgccc cctctcccgc cgtccgacgc gcgccgacgc gcgctccgac ccacgacagg
    22801 cggcccccgc tccacccgaa cggggcgggc gcgggaagga agaagcggtg cggccccatt
    22861 cgagcgtcga gggaggcgac gcgcccctgt gcggaggcga ggccggtctc cggatcggtc
    22921 cggagccgtg cgtcgatcgc gagcgtcccg tcgagaccgg cctcgggcac gagcccgagg
    22981 aggctcgagt aggcgcccag ggagaggacc tcgaggtcga gcgacacgtc cctgctcttc
    23041 ccgcgggccg tcagccgcgc ggtccccggc gcctcggcct cggcatcgag gtcgtcgccg
    23101 tcgagcgcga gccgcaccga gagcggctcc ccggcccggc cgagcgcctg gcctccgatc
    23161 gcggcctccg ccacccgtcc ctccagagag agcctcggct cctccagcgt gccgctcccc
    23221 tcgagcgtcc ccgtcagcag gcccgtgagg ccgggcgcct cgccggagag cgcaccgaga
    23281 cgctccagcg gcagcgccga ggcctcggcc ttcacctcga agccgtcgtc gtcgtagcgg
    23341 tagaggccgt cgagacgcgc gctcccggcc ccgagcgtcc cgctcacgcc ggagagacga
    23401 agcacctcgg cctcgaagcc gagcgtcccc gcgacccggt cgaagggctg gccccagagc
    23461 gtcgtctcct cgaagacgag gtcccccgcg ccgacgacgc gtggcgtgac gccggaaagc
    23521 ggcaaccggc ccgtgacgcg ccccgcgatc ggcaggtcga gagtgaggaa cgagaggacg
    23581 cgctctgcgg gccagcgctc ggtctcgagc gtcaggccgc tcaggcggta ttcgtccccg
    23641 agcgcccctc cccatccgac ctcgcctcgg acgaccatcc ggccgtctcc gtcccgcgcg
    23701 tcgagggcgt ccagcgcgag gacgttccgg tcgacgacga agtcggccgc cgtctgtccg
    23761 aatcggacgc cccggaggac gaattccgag gcctcgaggc gtccggcgat cctcgggtcg
    23821 gagaagctgc gtgcgagcgt cgcgacgagg cgcccgcttc cgcccaggtt cagcttcggc
    23881 aggagcggct ccccctggat cgcggggtag aagttctccg cgaggcgctc ggcctcgcgg
    23941 aggtcgggcg cgtcgatcgc cagcttccag tcgggctccc acgaaccgat ccggagcgtc
    24001 ccgtcgagga ccgccgtcag cccgcccgcc gtgacgaagg ggacccccgg gaaccgaatc
    24061 gcgccgttct ccacgaagag ggggccgccg ccggagacgg ggagcgcgtg gcgcccgcgg
    24121 acggccgacg gaacgccgtg cgcgggatgg agccggatca cgccggaacc gtcgctccgc
    24181 tcgacgcccc ccggcccgaa cgtgagcgtc atgtcgaggt cggtcctcgc catcagcccc
    24241 gtgccggaca ggccgaggtc cgagaagaac cgctcgaagt cgatctccct tcccgagacg
    24301 gcgatccgga ccggcagcgg cggacccttg atcctcgcga gctgaacgag cgcctcgagg
    24361 ttcccgcccg cgtacgtggc gcgggagacg tgcgcgagga ggccctcggg gtcgacccgc
    24421 aggaatccct ctcccgtcat cgggaacggg ccgaagctcc ccccctcctc gatctcgaag
    24481 cggccccgta tccggtagcc gcccccgcgg ggcacgcgga cgctcgccac ggcggacacg
    24541 ggtcccgaca gcgggacgcc ggcgccgaag tagcggtcca ggtcctctcc ccgcgcccgg
    24601 acgcgggcga ggaccttcac ctcgggatcc ttcaggtcgt cgatgccgcc gagggcgtcg
    24661 agagccaagc cgtcgccccg aagccggagg ccgcggagcc gcaggcgccc gggcgtgagg
    24721 acgacgtcgg cgccgacctc gaggtcgagg acgtcgccgt cgtcgagccg gaaccgggcc
    24781 ttgcggcatc cgagggaggc gcgcgtcgtc cgggagaacc ggttcgatcg cgccgtcaac
    24841 gcggcgtcgt cgaggacgac gtcgagcttc gatttccact cccggaaccg gatcgtcccc
    24901 ttctggagga ccgcctgccg gacgcggacg tcccggcctc ctccgcctcc cttcgggagc
    24961 gccgcggcga tggaggagaa gttgttcgtc ccgtccccga agacctcgag gacgatccgg
    25021 ggagagatga gcctcacctt cgggaggtcg atccggctct cggagacctg ggggatcccc
    25081 cgcaggctga tctcctccgc cgagaagcac gaccccggca ggcctcgcgg gtcgttcgcg
    25141 acgaagacgt ccttcacgag gaaggccggg ggcacgagag cgaggtcgaa gccgccgatc
    25201 cgcacctcgc ggccgatctt ctcggacagg agcgtctgcg tgcgccgacg caggagatcc
    25261 tggaaccgcg cggagcggaa gaccgcgtac aggacgagga tcgagaagac gaagaggagc
    25321 gcgaagagcc ggaggacccg gacctgctcg aggctcagct ggggaatctg ggggtggggg
    25381 atccgcccgc cgagcccctt tctcgccccc atcgcgacgg ccgcctaccc cttctcgccg
    25441 ccgaacgaga gcttcttggc gaggacgtcg aagaacgtgc ttcccggcgc cctcacgagg
    25501 agcgccgtct cccgcgcgcg ggcgatccgc acgcgcgtgg aggagccgat cgggaacccc
    25561 tcctgcccgt ccagcgtgag atagacgtcg gacggctctc cctcgacggc gaggccgagc
    25621 gtcacgtggt ccgagaggac catgggccgg agggagagcg tgtgggggca gatcggcgtc
    25681 aggatgatcg ccggcatcgt cggcgcgagg atcggtccgc ccgccgagag gttgtaggcc
    25741 gtcgagccgg tcggggtgga gacgatgacg ccgtcgcccc ggtagcgtag gacctggcgt
    25801 ccgtccacct cgacgcggat cgtcgcgatc cgggagaggg cggacttgcc gatcaccgcg
    25861 tcgttgacga cccggatcct ccggcgcggc cggcccggct ccacgacgtc gaccgagagc
    25921 aggcggcgcg tctcgacggg ggcggtgccg gcgagcgcat cggccaggag gggctcccag
    25981 gcgtcgggcg ggcaggcggt caggtagccg aacgtcccga tgtcgatccc gaggatcggg
    26041 acgcccggcg ccgggtgacg agcgaccgag aggagcgtcc cgtcgccgcc gagcgtgacg
    26101 aggaggtcga cgtgccggcc gatctccgcg cgcgggagtc cgcccatcgc cgagcgcgca
    26161 cccatcgact ccgcgtcgtg gagcacctcg acgccgcgct tgcgcagcca ggcctcgagc
    26221 cggcgggtga tgtggatcgc ctcgccgctc gtcgtccggg tgacgaggcc cacccgccgg
    26281 acgggcctcc ggcgcgcctt cggtcgcgtc gacatcggcc gattctagtc gctgccgctc
    26341 ggtggcgcgc cccgctggaa cgccgccagc agctccacgt tcccgtcggc gcccgtgatc
    26401 ggcgacggga cggtcccggc gtgggcgaga ccgagcgcgt cggcggccgc cacgacccgc
    26461 tcgagggcgc gggtcctcac ggccgggtcg cggacgatcc cgcctctccc cacctcgccc
    26521 cgctccgact cgaactgcgg cttgacgagg aggagagcct cggcacgcgg ggcgaggaga
    26581 ggcaccgcgg cggggaggat caggcgcagc gagatgaagg agacgtcggc gacgaggagc
    26641 gagcagggct ccggaacgtg ctcgcgcgag aggagccgcg cgttgacccc ttcgcggagg
    26701 acgacgcgcg ggtcggagcg gagcttcgcg tgcagctggc cgcgcccgac gtcgatcgcg
    26761 tagacgcgaa gcgccccgcg cgagaggagg acgtccgtga agcctccggt cgaggccccg
    26821 acgtcgaggc agatccgccc ggccgggtcg atcccgaacg cgtcgagccc tccggcgagc
    26881 ttgacccctc cgcgcgagac ccaggggtgc tcggggccct cgacggccag ctccgcgtcc
    26941 gcggggaggg gcgtcccggg cttcggccgc tcgacgccct tcaccctcac gcgccccgac
    27001 aggacgagcg cctcggctcg cgtccgcgac tcggccagcc cgcgcgcgac gaggaggagg
    27061 tcgagccgcg tcttcgacgg ggacatcaga tctcgacgac gaccttcagc gtcgcgcccc
    27121 cgccctcggc gagcgcgaac gcctcggccg tccgttcgag cgggagccgg tgcgtgacga
    27181 gcctctcgac cgggagggcg cccgaggcga tgaggtcgag cgcgcgacgg gtctcgaggg
    27241 gccccgagga gtagctgaag cggagcgtca ggtccttgaa gtacgggacg ttcggcgcga
    27301 tcggccatgt ctctcccggc gcgaccggag agaagaagac gacgcgaccc gccggcgccg
    27361 ccgcctcgac cgccggcagc accgccgcgg gcgacgtcgg gacgacgaag acggcgtcgg
    27421 ccccgcgcgg ggagacggcg tggaccgcct cggggaggcg ctcggcggac gggtcgacga
    27481 ccgcgtcgaa gccgtgctcg agggcgagac ggcgccggag gggatcgggg tcgctcccga
    27541 cgaggaggtc cgcgccggcc aggcgtgcga gccagccgag gaggagcccg ttggagccga
    27601 ggccgacgac gagcgcggcg tcgccggcgc ggacgagcgc gcggtcgacc gccttgagcg
    27661 cgcaggcgac cggctcgacg aacgcacccc cggtgaacga gacgtgcgga gggaggag
//
LOCUS       DAOTEZ010000006        24787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_1326_length_24787_cov_6.456817, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000006 DAOTEZ010000000
VERSION     DAOTEZ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 24787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24787
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1326_length_24787_cov_6.456817"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="P5164_00650"
     CDS             <1..206
                     /locus_tag="P5164_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010562622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRY42425.1"
                     /translation="GTIRLNTELVKKPKDLLEYVVVHELAHLVEPTHSERFVELMTRH
                     YPAWRVARAELNELPLAAEVWRE"
     gene            complement(299..1729)
                     /locus_tag="P5164_00655"
     CDS             complement(299..1729)
                     /locus_tag="P5164_00655"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRY42426.1"
                     /translation="MPRSFYLIAIGGTAMTPLAALLVEKGERVLGSDLPLYPPMSDRL
                     EALGIDVLPGFDARNLPPRVDRVVVGNLAGKDNPELVAALDRGLPVASMPETLRTEFL
                     AGRHPVVVAGTHGKTTTTALTAWLLAAAGRSPGYLVAGEPRNFPSPSAIGTGEAFVVE
                     GDEYSTSWEDKGPKFLHYAPRTFLLTSVEFDHADLYADLDAVKEAFRAGVAIVPPDGR
                     IVACGDDRNVRDVLGGARARVVLYGLGDAPDLDLRASGIEEGPEGTRFVVHPRGRAPF
                     EVRSPLAGRHNVANALAALAAAEGLDVSAERLAAGLPSFLGVKRRLEVRATAAGVTVV
                     DDFAHHPTAVATTLAGARRRWPGQRVWGVFEPRSLTAGRADFFEPYLAALREADAVAI
                     AAPYHAARLSRPGAPGALDVGTLVARLAAEGKEAFTAPDPDALAGALLPRLSEGDVVV
                     VMSSGAFGGLCGKLVQALATREVAAV"
     gene            1855..2907
                     /locus_tag="P5164_00660"
     CDS             1855..2907
                     /locus_tag="P5164_00660"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRY42427.1"
                     /translation="MTSPAFRIRSGRTAAALVAALLAFAPAALAQAPKPSTAAAPKLV
                     LVEEKKDVGTVPKGEVIHATFVFRNEGKADLHITDVKPSCGCTAPEYDKTVKPGAQGK
                     IVLNVDTKTFQGPISKSALILTDDPEKSSVTVFVVANVKPYVETHPVGFFRLQALTGE
                     TASAELVLGSDEADFAPTKVETPNSYLKATLAPVPDAEKVAGKGARQWKAVVTTTPDA
                     PEGLLGGYVKIATGAAKQPELDLAVSGYIKPTLSVTPLSVNFGNFEPKADPVKRTVTI
                     INNNLKNEAFQVTKAEANVPGVKTEVVMADKSRAQVIISVDDKVKKGVFDGFVTVSTT
                     DPAKKEIKIPLKGVIL"
     gene            complement(2971..3516)
                     /locus_tag="P5164_00665"
     CDS             complement(2971..3516)
                     /locus_tag="P5164_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42428.1"
                     /translation="MRAGRLAPGLALALSLAACGGNAPEAVFLNFDPLTTPRELTRGW
                     SSFERTGAGDTYVWAQARAATVTLRAGRPDDRIVRFRAWPFRYEGAPQQRVTVVLNDV
                     TLGTFPLPDGPTVVETPSPAAAWKAGKNVLTFDFAYAEAPKERIAGADDGRTLAAGFD
                     WLEAVPVPADGAPSRPSPQRP"
     gene            complement(3513..4742)
                     /locus_tag="P5164_00670"
     CDS             complement(3513..4742)
                     /locus_tag="P5164_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42429.1"
                     /translation="MRRAFAAALLLVLGGAAFADTPATTQWVLATAKATGAGGEQFVS
                     SLRISNPFAAAANVDLTYLAQSPIDGTGSASGDNAAAPKVRVTVGPGETLALEDVLGT
                     TFAGKAAPFGISAGGIRVDSDVPVAVLSRTYVANARSAAGVPGTFGFSIPAQVEAQAL
                     SAGETGWLTFVAASPTNASGFRTNLILLNTGAAPAVLHVALLRADGSVAGERDYTLGR
                     TSAAQQGNLGAAFGVASSETNLRAAVTVKSGGPVAFGASLIDNAISSIAYLPPVKVDA
                     PDDGAYGWVVTRGDPALASAGRLDIAGGAPNFLSGLLVVECPAGSYLYTVLAYGAGAG
                     VPDPNTTFAPRAEGGFSFTGSAPNAGSWSGTVSPRFDGTLLGTLTFSPPAGSRCAGTT
                     RAFDFAGSRAASFELTP"
     gene            4845..5450
                     /locus_tag="P5164_00675"
     CDS             4845..5450
                     /locus_tag="P5164_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRY42430.1"
                     /translation="MEKKGVGRPRDGNPEETRREILAAASEAFAAAGYVGATTRAVAS
                     RAGVNVATLHYHFGSKEGLYRAVLEAAGRGALPTIPPGDAPETVARVVEALFRFGEER
                     PGLARLALLDALAGPEGGPGPGEERVAWAAEALRPHLGGADGADEARVVVVLVDAAQA
                     TSPAPSAAAGETSEAGNAGIHPPERVRRAVVAAALRLTALG"
     gene            complement(5434..6783)
                     /locus_tag="P5164_00680"
     CDS             complement(5434..6783)
                     /locus_tag="P5164_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42431.1"
                     /translation="MIPALLVYGAAVLLVGLFAARRAARSAESFLLGDRSFGFLPTWA
                     GLTSTVIGGSTTLVLASLVAEKGLPGAWLDIAGALGLLGLGLFLARRVRETGEVTLAA
                     VVGRFYGPRVRKVAAVLVVLAEIVWFALLAQATETVVSALTDWPPTPVLVGTALLFVA
                     YTALGGQRAVLRTDLLQLGLMVVGIVGVAFPLALLSLARTGWPEPLLSFPVGARFGWS
                     DVGALLVLVGLPHAVGSDVWAKLLSARDATTARRAALGAAAAKLAFGAAVVVIAFAGV
                     ALDVGGAPQAIFPGTVRVLAGPLLSPLLLVALVATMQASADSVLLSAAAATSHDLLGG
                     APARTSRLLVVAYGALGLLVALGMRDVLATFRLGYAIFASSLILPTLVAFAPRLRLRP
                     RFAGAAMLLGGATTLAATLVGARLDPVLLGTAVNALVLLPGLSIRGAERDPTVSRGR"
     gene            6813..7121
                     /locus_tag="P5164_00685"
     CDS             6813..7121
                     /locus_tag="P5164_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 family protein"
                     /protein_id="HRY42432.1"
                     /translation="MKRTFTFDEARKVLPAVRERTRRAVARIEALGEPWEAPAGPEAR
                     AILLSWAREMEELGIEVKGPGLVDFDSGDGYWCWRWPEEALDYYHGYDEGFAGRVRIQ
                     "
     gene            complement(7108..7611)
                     /locus_tag="P5164_00690"
     CDS             complement(7108..7611)
                     /locus_tag="P5164_00690"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRY42433.1"
                     /translation="MRDVRLPEFPCVVPVEVRFRDLDAMGHVNNAVYLTYFEQARLAF
                     WLAIHPGGAPGEAVAPERIGFVVARAECDYASPVRLGERLLVGCRAGDFGTTSFAFDY
                     RVVAAGSSVDAEVRVVASGRTVQVGWDWKEGRKSPLPEELRRRIDTFQAGISRPGARS
                     GHAVTGS"
     gene            7657..8985
                     /locus_tag="P5164_00695"
     CDS             7657..8985
                     /locus_tag="P5164_00695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY42434.1"
                     /translation="MARSPNLDDDLDSLLDRALRGEGEIDESLRARLSQAFPALRVPL
                     PPAEGRAAAPKTPAAPHAAPHAAESTGPIEAPGANAPSFAEALPLGIVLSDASGLVRS
                     ANAMARRIFGAEAVALEGRRVIELFEAVERAQLAGLLEDAFRGRPTREVALNAANPAG
                     AVAVRVSAAGRFGPDRKLTQVVWTVREAGVNPAALEVHVHSGKQELLAELGMELGREV
                     NPLLVRLTESLVSVQRVLSSGGDEPPAEREAIRVKIADALSGLLRLSEVVTELERFSA
                     TAPLKIEAVDPSEILERAQKLLGRRLKGAKLRVRNDMDEPAPKVLADAPRTVEIFVNL
                     LRNARNAILRRFEGADPEASAAMKRLVVVQAFVRDSYVALVFTNNGVPVPQADHERIF
                     LPSFQARDPERSGLGLPETAALIKQMGGAIRCEPLGEQGTRFILTLPKAS"
     gene            complement(9100..9651)
                     /locus_tag="P5164_00700"
     CDS             complement(9100..9651)
                     /locus_tag="P5164_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HRY42435.1"
                     /translation="MSRTPAILLRRDCAAVRVPSGEGALLPEGVPVYLVQSLGGSFTI
                     QAPTVGGLFRIGPEDADALGLVPSSADPPPKRAPKGPASEQEVWAALRQCYDPEIPVN
                     IVDLGLVYDLTLSPAKGRGTQVAVKMTLTAPGCGMGPAIAGDARARLLAVPGVVDAEV
                     SLVWDPPWSAEMASPEGRRVLGV"
     gene            complement(9648..10838)
                     /locus_tag="P5164_00705"
     CDS             complement(9648..10838)
                     /locus_tag="P5164_00705"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY42436.1"
                     /translation="MTRRLSPLAVVFLTVFIDLVGFGIVIPLVPLYAEAYHPSPLAFG
                     VLMASFSAMQFVFAPLLGRLSDRVGRRPVLLFSLFGSFLSYLLFAFAGSFGGLLASRV
                     LAGIMGANIGTAQAVIADVTPQGDRARGMGLVGMAFGLGFVFGPAIGGFSVKVSHTLP
                     GLVAAGLSLVAWLWAYFGLPETRPASAAARPPRHWFPLRAVLNAFARPVVGPLLLVTL
                     LNTTAFANFEATFAQFARSRFGHDPSTVSWLFVLIGVTAAVVQGGLIRRLVPLLGEAR
                     LIGIGSTLLALSFAGLVLVPGHGALVALVVTTAAGSGLVNPALAGLLSRSTEAAEQGE
                     ILGAAQSLSSLGRIVGPFWGEHAWFAWDAAGPYGTGALLEVAVVVVVLVGLKAVSSPR
                     TRPA"
     gene            complement(10920..11291)
                     /locus_tag="P5164_00710"
     CDS             complement(10920..11291)
                     /locus_tag="P5164_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015485503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HRY42437.1"
                     /translation="MASTTLNTATVSVQETFALSLTDRAVDKVKDFAAKMPDAADKAL
                     RVFVQAGGCSGFQYGFTFDDAKETDHVLAVGGIRVLVDKTSAMKLNGATVDWIEDFRG
                     AGFSVENPNAGGGCGCGKSFA"
     gene            11534..14341
                     /locus_tag="P5164_00715"
     CDS             11534..14341
                     /locus_tag="P5164_00715"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF005122.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HRY42438.1"
                     /translation="MSFPADRTAEQASVSTASEPAASRSALRGLTFRRLYTDGVTHPF
                     DAIEWELRTAAITNEKGEVFFEQKDVEVPKAWSMTATNIVTQKYFHGKVGTPERERSV
                     RQLIGRVVETITGYGSKGGYFRTAADRDAFRDELAAILVNQAASFNSPVWFNVGVEEK
                     PQASACFINAVGDSMGSILDLAKTEGMLFKFGSGTGSNLSSLRSSTECLSSGGIASGP
                     VSFMKGFDSFAGAIKSGGKTRRAAKMVILNVDHPDVLEFIDCKAKEERKAWDLIEAGW
                     DGSFGGEVYNNVLFQNANHSVRVTDEFMKAVEEGREYACHAVTTGEELLKLDARAVLR
                     RMADSAWICGDPGIQYDTTINRWNPCKSTHRINASNPCSEYMFVDDSACNLASLNLMK
                     FAKEDGGLDVEKFRHAVDVVFAAQEMLVDEASYPTPAIEKNSHDFRPIGLGYANLGAL
                     LMYNGLPYDGDDGRAYAAAVTSLMHGQAFLTSSRIASEIGTFRMYEPNRDAFLGVIGM
                     HREAAAQVPRTGVPKELYEAQLGVWDLTLESGRQHGYRNAQATVLAPTGTIGFMMDCD
                     TTGVEPDLALVKYKKLVGGGTIKIVNNTVPHALARLGYSEPQVNEIVAYVDEKGTIEG
                     APHLSAAHLPVFDCAFKARGGTRSIDAMGHVRMMAAVQPFLSGAISKTVNLPPDATVE
                     DITNVYVEGWKLGLKAIAVYRDGCKRTQPLNTAASKSDDAVKGTKGAAAAPAPAPAAT
                     AGPRRKKLPDERRALTHKFSVGGHEGYITVGLYEDGQPGEIFLTMAKEGSTISGLMDA
                     FATAISLTLQYGVPLEALVEKFSHMRFEPAGYTKNPEIPIAKSLVDYIFRYLASRFLT
                     ADLRARAGVISREENGNGNGHALAAIPVPPAQKAAAASATTLAFENSADAPSCHQCGH
                     LMVRNGACYKCLNCGETSGCS"
     gene            complement(14547..14951)
                     /locus_tag="P5164_00720"
     CDS             complement(14547..14951)
                     /locus_tag="P5164_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42439.1"
                     /translation="MRGHTRIGLLATGFSLFLLFGCVSTTWHPGEDRAPGVRPSVSPA
                     EVAVLRSLPKAPHQSLGTIEVWISGRHSSAAILGKVREKAAAVGADAVVLEPGATLFT
                     EPMSIENEVVGPQAVSYSFTAIRHLESPPSSS"
     gene            complement(15073..15504)
                     /locus_tag="P5164_00725"
     CDS             complement(15073..15504)
                     /locus_tag="P5164_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42440.1"
                     /translation="MDRKYRQRGYQDNAPSTPRSGGPFDDRPARLEGAPRGRSAGRPS
                     EEVFRCRTCHEKNDPAVALAALCVKCRAPLHACAQCRHFDGGAPFQCRQPIPAPISAK
                     SRANECSFYEPAVTLDLTGRKATDTPDQARSAFDALFGKKK"
     gene            15596..15958
                     /locus_tag="P5164_00730"
     CDS             15596..15958
                     /locus_tag="P5164_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017622660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRY42441.1"
                     /translation="MKLILRVLVNALALGAAAYLVPGIHAGGVESVLLIALVFGVLNA
                     LVRPFLKLLSCPLLVLTLGLFTLVLNAAMLMLTAWLGQKLGIDFRVDGFWPAFLGALV
                     VSIVSVVLSWVLIDEKKG"
     gene            16168..18057
                     /gene="aroF"
                     /locus_tag="P5164_00735"
     CDS             16168..18057
                     /gene="aroF"
                     /locus_tag="P5164_00735"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRY42442.1"
                     /translation="MAERNEAEGREGACEQRNRRAGYQGRESSFGARAAARCGRPVAL
                     PSFEALLDALVAGAVDEALLPAVDRVIGPVVEALGPLGAAVDRGARFAVLGEVEVPMR
                     LVLAAPRGVALEEIEELHSQPPVLRQCAGLLARLGARAVPTEDTAQAAVNVARAGGRR
                     GAVCSEEAAFEAGLVPLLEDVSDVRPNGTRFWRVARSDDALLGESPLRVLRVTTERLG
                     AHLRQAGVEVLASGPGSGFALVGDAQVAAAAAAVREAKAKGATWLGTPPDLVRRPRVE
                     ALPRATEVRPPTVVTVGGFRVGGGARAVIAGPCTVESEEQIGRLARAVARAGATALRG
                     GVFKPRTSPYSFQGHGLHALRWLKEAGESVGLPVVTEVMAPAQVGPVAAVADVLQIGA
                     RNCQNYDLLKEAGAAGRPILLKRGFGTTVDEWLASAEYLLDAGGAEVMLCERGIRTFE
                     HATRGTLDLGGLLAARQLTHLPLLADPSHSSGRRELVPGLSRAAWGAGVDGLIVEVHD
                     APESAWCDGPQALLPEDLERLCDELGLLPGATPSIERVRTAIDAVDREIARLVARRLE
                     LCRRVAEAKRAAGVPLRDAAREEMVTARYLETPGMDEGSARRLAESLIAMGLTVEGWS
                     AEGAA"
     gene            complement(18064..18483)
                     /locus_tag="P5164_00740"
     CDS             complement(18064..18483)
                     /locus_tag="P5164_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRY42443.1"
                     /translation="MRSYRRELTFHLPKRIGFVNVTPEVEAAVRESGISEGLCLVNPM
                     HITASVFVNDDERGLLADFERFFEALVPHEPVRSWRHNDTGEENADAHVKRQLMGREA
                     VLAVTGGRLDLGPWEQVFYGEWDGRRAKRVLIKIIGE"
     gene            18597..19067
                     /locus_tag="P5164_00745"
     CDS             18597..19067
                     /locus_tag="P5164_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRY42444.1"
                     /translation="MTEAVEERRLFADFLRRRGLKTTRERTALFEEIFATHHHFDADD
                     LVVRLRGKGKKVSRATVYRTLDLLYDCGLVSRVRLNDEKYRYERLLVGEHHDHLVCTS
                     CGAVIEFVDPRIEELQETVCKKYGFAPTTHSHQIRGVCSDCQAREGTSRGASPA"
     gene            19077..20516
                     /locus_tag="P5164_00750"
     CDS             19077..20516
                     /locus_tag="P5164_00750"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HRY42445.1"
                     /translation="MAPAIVWFRRNLRLDDNVPLDAALRGHETIVPVLVLDDHYRDED
                     FSPPRLRFLAESLRELEAELAARGSRLLVRNGPAGDALAALARETGAEAVYGHDDHEP
                     HGRALAREVDAALAWQGTRLRLLPDLHLVSPGSLRTDSGRPYAVFTPFLKRWREADKA
                     APRPTPPRVPTPDAVLDTSFPSIPLSRPRRLREVGAPANPPGGAREARHLWDGFRESA
                     LVRYEGGRDVPAASGTSRLSPHLRFGTIGIRRLLAEARAAWKDADAPGRASIDVFVAE
                     LAWREFYASILAENPRVLTESFRPEFDGFPWSAGEEAEERFAAWRDGRTGYPIVDAGM
                     RQLAHEGWMHNRVRMIVASFLTKHLLVDWRRGEALFRARLADGDPASNNGGWQWSAGC
                     GTDAQPFFRIFNPVLQGERFDPDGAYVKRWVPELAAWKGAGRNLHAPWRAPSPPADYP
                     APIVDHATARARALAAFDAIRGAAGSPRR"
     gene            complement(20485..22521)
                     /locus_tag="P5164_00755"
     CDS             complement(20485..22521)
                     /locus_tag="P5164_00755"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRY42446.1"
                     /translation="MEGGSVAGPFPDLTPLGLPDLAPFLTLEAAADPASRAGSGLLVS
                     RLLGFSAGTRAEPLARAALVSAARAARTPAERRAVLDALAARERLGPGTPRGRISAAL
                     VRTRLARTPAEAVDQMANLLRTFPDAPETAPDLFEPADHALLSRLLSRAAPELKLVRA
                     RALARRSPSAAADLAAPLARLPGYRLDTADLLLAAGRARAARDALVKPSALAGRDDGE
                     RLRIEVVLLAAEFRLLDEPTRRTPAPRHRRAPRPAPAVPGAPPAPPDAETLRAWASLA
                     ARSEALLARPLAAMDRRRLLGEAVRAAARFGQLEAARRFLPALVAIDPATTVGAEELF
                     RAAFLPTLTRTPEAHRAAAAALEEQAALYGDVAVRRRAIYWAARSLEATGKGAAARAL
                     QASLVSTAVPDLYARWAGARLGIPVRAALPLDPPGPPLPHALRAEAPSLPSRELLAVG
                     LASLAEDAAEEERSADPLFLAACASERFEFRRAVGILKSRWPELGTPDEGALPLAVRR
                     AYYPFRQEALIVREATANGLPPALVYGVIRQESLFQTGARSHAGATGLMQVMPGTGRD
                     LLRREGRRGRADLKDPEVNVALGARYLAMMLREFDGDRIAALAGYNAGPGRPRRWRRQ
                     APALAADEFLEAMPLFEPRDYVKRVLFFEAAYAVLHGAAIDPLPVSGGATRPRP"
     gene            complement(22716..24488)
                     /locus_tag="P5164_00760"
     CDS             complement(22716..24488)
                     /locus_tag="P5164_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42447.1"
                     /translation="MPVDPAANRRFIESLSTLLEDHLATWKSQLADTRNEIDQLISKA
                     SESIPAQARHLFPEDLVATLLEDAVPPAPAPERVVEKVVERVVETVTVPGPPAAPDWG
                     LVKSSIAAIEGSRTQVDVLTHFLTEAQVHSSRAALLVLRNDRLTGWKALGYDASGGRD
                     DSVKGLDLAAGDDPFVEACLHAEKPMVAVPPPESGALRRALGGHPPARTLLVPMVIRD
                     RIAGLLCADELPGEEGRLNESAIEVLTFVTGLSVDLLAARKKIPSPALTPRGEEVRTF
                     QPAIAAAAEPAPESEPAPAPAPAPPVSAAMAAPTAPPAPPAPAPAPPEAPPASASLSI
                     TGDLPGRSVFEQTDPSSARKTRRITDAGEALRALEESATTKKKDPFSGVDILTASGAR
                     SAAALGLATPRPGSSAPELTPRLATPTPAAPPNRGDLTMAFEQIRTVTPPPPPAPAPP
                     TPAPTPRPPAPPAAVAAAAPPGGLDVPAGSAASPLAPPPGFVPRSRAPRGGDDPQRAI
                     DDARRIARLLVSEIKLYNERKVAEGRVACDLYERLKDDIERSRQVYAERTPEHVRATS
                     DFFQEELVRILAEGKPEALGPIPS"
     gene            24670..>24787
                     /gene="rpsU"
                     /locus_tag="P5164_00765"
     CDS             24670..>24787
                     /gene="rpsU"
                     /locus_tag="P5164_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRY42448.1"
                     /translation="MPLIHVKEDESFENALRRFKRKCEKSGILSELKKRQHYE"
BASE COUNT         3545 a         8706 c         9438 g         3098 t
ORIGIN      
        1 cgggaacgat ccgcctcaac accgagctcg tgaagaagcc gaaggacctc ctggagtacg
       61 tcgtcgtcca tgagctggcg cacctcgtcg agccgacgca tagcgagcgc ttcgtcgagc
      121 tgatgacccg gcactacccg gcctggcggg tcgcccgggc cgagctgaat gagctgccgc
      181 tcgcggcaga ggtgtggagg gagtgagctg cgccggatag cggtcgacgc ggcaccgtgc
      241 gccgttttcg gtgaaggtgg cgaaggaagc ctgggcgatc ccgggcccgc gacccgagtc
      301 agaccgcggc gacctcgcgc gtcgcgagcg cctggacgag cttcccgcag agccccccga
      361 aggcgccgga ggacatgacg acgacgacgt ccccctcgga gagccggggg aggagcgccc
      421 cggcgagggc gtcgggatcg ggggccgtga aggcctcctt cccttcggcc gcgaggcgcg
      481 cgacgagcgt tccgacgtcg agcgcgccgg gggcgccggg ccgggagagg cgggcggcgt
      541 ggtacggggc ggcgatcgcc acggcgtccg cctcgcggag cgcggcgagg tagggctcga
      601 agaagtcggc gcggccggcc gtgagggagc gcggctcgaa gacgccccag acgcgctggc
      661 ccggccagcg tcgccgcgcc ccggcgagcg tcgtggcgac ggccgtcggg tggtgggcga
      721 agtcgtcgac gaccgtcacg ccggcggcgg tcgcgcggac ctccagccgg cgcttcacgc
      781 cgaggaagct cgggagcccc gccgccaggc gctcggcgga tacgtcgagc ccttcggccg
      841 cggcgagcgc cgcgagcgcg ttcgcgacgt tgtggcgacc cgcgagcggc gagcggacct
      901 cgaacggggc tcggccccgg gggtggacga cgaagcgcgt cccctccggc ccctcctcga
      961 tgccgctggc gcgcaggtcg aggtcgggcg cgtcgccgag cccgtagagg acgacgcggg
     1021 cccgggcccc gccgaggacg tcgcggacgt tccggtcgtc gccgcacgcg acgatccgtc
     1081 cgtcgggcgg gacgatcgcg acgccggcgc ggaacgcctc cttgacggcg tcgaggtcgg
     1141 cgtagaggtc ggcgtgatcg aactcgacgg aggtgaggag aaacgtccgg ggggcgtagt
     1201 ggaggaactt cggccccttg tcctcccacg acgtcgagta ctcgtccccc tccacgacga
     1261 acgcctcccc ggtgccgatc gcggagggag aggggaagtt ccgcggctcg ccggcgacga
     1321 ggtagccggg ggagcgcccc gcggccgcga ggagccaggc cgtcagagcc gtcgtcgtcg
     1381 tcttcccgtg ggtcccggcc acgacgaccg ggtgccggcc ggcgaggaac tcggtccgga
     1441 gcgtctcggg catcgaggcg accgggaggc cccggtcgag agcggcgacg agctcggggt
     1501 tgtccttccc ggcgaggttc ccgacgacga cgcggtcgac cctgggcggg aggttgcgcg
     1561 catcgaagcc gggcaggacg tcgattccga gagcctcgag ccggtcggac atcggcgggt
     1621 agagcggcag gtcggagccg aggacccgct cccccttctc gacgaggagc gcggcgagag
     1681 gggtcatggc ggttccgccg atggcgatga ggtagaagct gcgaggcatg ggcgcgcagt
     1741 ttgctttccc gttcccggca ccgtcaaccg cgccgggatg gccccggggc ccggacgcct
     1801 ataatcagag gttcgagtgg cggccggtcc gccgcgaaca ggaggtcttc ctcgatgacg
     1861 tctcccgctt tccgcatccg gtccggccgg acggccgccg ccctcgtcgc ggcgctcctc
     1921 gcgttcgccc ccgccgccct cgcccaggcg ccgaagccgt ccaccgcggc cgcgccgaag
     1981 ctcgtcctcg tcgaggagaa gaaggacgtc gggacggtcc cgaagggcga ggtcatccac
     2041 gccaccttcg tcttcaggaa cgaagggaag gccgacctcc acatcaccga cgtgaagccc
     2101 tcctgcggct gcacggcccc cgagtacgac aagacggtca agccgggcgc ccaggggaag
     2161 atcgtcctga acgtcgacac gaagacgttc cagggcccga tctcgaagtc cgccctcatc
     2221 ctgacggacg atcccgagaa gtcctcggtc acggtcttcg tcgtggcgaa cgtcaagccg
     2281 tacgtcgaga cccacccggt cggcttcttc cgcctccagg ccctgaccgg ggagacggcg
     2341 agcgccgagc tcgtcctcgg ctcggacgaa gccgacttcg cgccgacgaa ggtcgagacg
     2401 ccgaacagct acctcaaggc gactctcgcc ccggtgccgg acgccgagaa ggtggcgggc
     2461 aagggggctc gccagtggaa ggccgtcgtc acgacgacgc ccgacgcccc ggaggggctc
     2521 ctcggcggct acgtgaagat cgcgaccgga gccgccaagc agccggagct cgacctcgcc
     2581 gtctcgggct acatcaagcc gacgctttcc gtcacccccc tgtcggtcaa cttcggcaac
     2641 ttcgagccga aggccgaccc ggtcaagcgg acggtgacga tcatcaacaa caacctgaag
     2701 aacgaggcct tccaggtgac gaaggccgag gcgaacgtcc cgggcgtgaa gaccgaggtc
     2761 gtcatggccg acaagagccg ggcccaggtc atcatctcgg tcgacgacaa ggtcaagaag
     2821 ggggtcttcg acgggttcgt cacggtctcc acgaccgacc ccgcgaagaa ggagatcaag
     2881 atccccctta agggcgtgat cctctaagcc cttcttccgg cgtcgacgcg acgggccggg
     2941 cggacgcgag tcctcccggc cctttccgtc tcagggccgc tgcggcgacg gccgcgacgg
     3001 ggcgccgtcg gcggggaccg ggacggcctc gagccagtcg aagccggcgg cgagcgtccg
     3061 gccgtcgtcc gcgcccgcga tccgctcctt cggcgcctcg gcgtacgcga aatcgaacgt
     3121 caggacgttc ttcccggcct tccaggccgc cgcgggggac ggggtctcga cgacggtcgg
     3181 gccgtccggg agcgggaacg tgccgagcgt gacgtcgttc aggacgaccg tgacccgctg
     3241 ctgaggcgcg ccttcgtacc ggaacggcca ggcgcggaag cgtacgatcc ggtcgtcggg
     3301 acgcccggcg cggagcgtga ccgtcgctgc gcgcgcctgg gcccagacgt atgtgtcgcc
     3361 ggcgcccgtc cgctcgaacg acgaccatcc tcgcgtcagc tcgcgcggcg tcgtcagcgg
     3421 gtcgaagttg aggaagacgg cctccggagc gttcccgccg caggctgcga gcgaaagcgc
     3481 gagcgccagg cccggcgcga gccgcccggc cctcacggcg tgagctcgaa ggaggccgcc
     3541 cgcgacccgg cgaagtcgaa ggccctcgtc gttccggcgc atcgggaccc ggccggcggg
     3601 gagaacgtca gcgtgccgag gagcgtcccg tcgaaccggg gcgagacggt ccccgaccag
     3661 ctcccggcgt tcggcgcgga gccggtgaag gagaagcccc cttccgcccg cggggcgaac
     3721 gtcgtgttcg ggtctgggac gcccgcgccg gcgccgtagg cgaggacggt gtagaggtac
     3781 gagccggccg ggcattcgac gacgaggagg ccgctcagga agttgggggc gccgccggcg
     3841 atgtcgagcc gccccgcgga ggcgagggcc gggtctccgc gcgtgacgac ccagccgtag
     3901 gccccgtcgt ccggggcgtc gaccttcacc ggcggcaggt aggcgatgga ggagatcgcg
     3961 ttgtcgatga gagacgcgcc gaaagcgacc ggcccgccgc tcttcaccgt cacggcggcc
     4021 cggaggttcg tctcggacga ggcgacgccg aatgcggcgc cgaggttccc ctgctgcgcc
     4081 gccgaggtgc ggccgagcgt gtagtcgcgt tcccccgcga cgctcccgtc ggcccgcagg
     4141 agcgcgacgt gcaggacggc cggcgccgcg ccggtgttca ggaggatcag gttcgtccgg
     4201 aagccgctcg cgttcgtggg cgacgcggcg acgaacgtca gccagcccgt ctctccggcc
     4261 gagagcgcct gggcctcgac ctgcgcgggg atcgagaagc cgaacgtccc ggggacgccg
     4321 gcggcgctcc tcgcgttcgc gacgtaggtc cgggagagga cggcgaccgg cacgtccgaa
     4381 tcgacccgga tcccgccggc cgagatgccg aagggcgcgg ccttcccggc gaacgtcgtc
     4441 ccgaggacgt cctcgagggc gagcgtctcg cccgggccga ccgtgacccg gaccttcgga
     4501 gcggccgcgt tgtcgcccga tgcgctcccg gtcccgtcga tcggactctg ggcgaggtag
     4561 gtcaggtcga cgttcgcggc cgcggcgaag gggttcgaga tccggagcga gctgacgaat
     4621 tgctcgccgc cggcaccggt cgccttcgcc gtggcgagga cccactgcgt cgtggcgggg
     4681 gtgtccgcga aggcggctcc gccgaggacg aggaggaggg cggctgcgaa tgcgcgtctc
     4741 atcggggtct ccttgccggg agccggcgga cgatcttaga ccgcggcgcg gaaccgggcg
     4801 gctgcgggga aatccggagg agccgttccg acggaaaccg gtcgatggaa aagaaaggcg
     4861 tcggcagacc ccgggacggc aatcccgaag agacccgtcg ggagatcctc gcggcggcgt
     4921 cggaggcgtt cgcggcggcc ggctacgtcg gggcgacgac gcgcgccgtc gcctcccgcg
     4981 ccggagtcaa cgtcgcgacg ctccactacc acttcgggag caaggagggg ctgtatcggg
     5041 ccgtcctcga ggctgccggc cggggcgccc tgccgacgat tcccccggga gacgctccgg
     5101 aaacggtcgc gcgcgtcgtc gaggcgctct tccgcttcgg ggaagagcgt cccggcctgg
     5161 cgcgcctcgc cctgctcgac gcgctcgcgg ggccggaggg aggcccggga ccgggagagg
     5221 aacgcgtcgc ctgggcggcg gaggcgcttc gcccccacct cggaggcgcc gacggcgcgg
     5281 acgaggcccg tgtcgtcgtc gtcctcgtcg acgcggccca ggcgacgtcc ccggcgccgt
     5341 cggcagcggc aggcgagacg tccgaagccg ggaacgccgg catccatccc ccggaacggg
     5401 tgaggcgggc cgtcgtcgcg gccgcgctga ggctcaccgc cctcggctga cggtcggatc
     5461 gcgctccgcg ccccgaatcg agagaccggg gaggaggacg agcgcgttca cggccgtccc
     5521 gaggaggacc gggtcgagtc gcgcgccgac gagcgtggcg gcgagcgtcg tcgctccgcc
     5581 gaggagcatc gccgcccccg cgaaccgcgg ccggagcctc agccgcgggg cgaacgcgac
     5641 gagcgtcggg aggatcaggc tggaggcgaa gatcgcgtag ccgaggcgga acgtggcgag
     5701 gacgtcacgc atcccgagcg ccacgaggag gccgagcgcc ccgtaggcca cgacgaggag
     5761 gcgggaggtg cgggcgggcg ccccgccgag gaggtcgtgg gaggtggcgg ccgcggccga
     5821 caggaggacc gagtcggcag aggcctgcat cgtcgcgacg agcgccacga ggaggagcgg
     5881 cgagaggagg gggcccgcca ggacccgaac cgtgcccggg aagatcgcct gcggcgcgcc
     5941 gcccacgtcc agggccaccc cggcgaacgc gatcacgacg acggccgcgc cgaacgcgag
     6001 cttcgcggcg gccgcgccga gcgccgcacg ccgggccgtc gtcgcgtcgc gggcggagag
     6061 gagcttcgcc cagacgtcgc tgccgacggc gtgcgggagc ccgacgagga cgaggagcgc
     6121 gccgacgtcg ctccagccga accgcgcccc gaccgggaag gagaggaggg gctccggcca
     6181 gccggttcgg gcgagggaga ggagcgcgag cgggaacgcg acgccgacga tcccgacgac
     6241 catgaggccg agctggagca ggtccgtccg gaggacggcg cgctgcccgc cgagcgcggt
     6301 gtaggcgacg aagaggagcg ccgtcccgac caggacgggc gtcggcggcc agtccgtcag
     6361 cgcgctgacg acggtctcgg tcgcctgcgc gaggagcgcg aaccagacga tctcggcgag
     6421 gacgacgagg accgctgcga ccttccggac ccgcggcccg tagaaccggc cgacgaccgc
     6481 cgcgagcgtc acctcccccg tctctcggac gcggcgggcg aggaagagcc cgaggccgag
     6541 gagcccgagc gccccggcga tgtcgagcca cgcgcccggc aggcccttct cggcgacgag
     6601 ggaggccagg acgagagtcg tcgagccgcc gatcaccgtg gaggtgaggc cggcccacgt
     6661 cgggaggaag ccgaaggagc ggtcgccgag gaggaacgac tcggccgagc gggcggccct
     6721 gcgagcggcg aaaaggccga cgaggaggac ggccgccccg tagacgagga gcgccgggat
     6781 cacgtgccga ccccgcgggt tacgatcggg gcgtgaagag gaccttcacg ttcgacgagg
     6841 cgcgcaaggt gctgccggcc gtccgggagc ggacgcggcg cgcggtcgcg cggatcgagg
     6901 cgctcggcga gccgtgggag gcgccggccg ggccggaggc ccgcgcgatc ctcctctcct
     6961 gggcccgcga gatggaggag ctcgggatcg aggtgaaggg ccccgggctc gtcgatttcg
     7021 actcgggcga cggctactgg tgctggcggt ggcccgagga ggcgctcgac tactaccacg
     7081 gatacgacga agggttcgcg gggcgcgtca ggatccagtg acggcgtggc ctgaacgagc
     7141 cccgggacgg gagattcccg cctggaacgt gtcgattcgc cggcgcagct cctccgggag
     7201 gggggacttc cgcccctcct tccagtccca gccgacctgc accgtccggc ccgacgcgac
     7261 gacgcggacc tcggcgtcca cggagcttcc ggcggcgacg acgcggtagt cgaacgcgaa
     7321 cgacgtcgtc ccgaaatccc cggcgcgaca gccgacgagg agccgctcgc cgaggcggac
     7381 cggcgacgcg tagtcgcatt cggcgcgcgc cacgacgaag ccgatccgct cgggcgcgac
     7441 cgcctctccc ggcgcgcccc ccgggtggat cgccagccag aatgcgaggc gggcctgctc
     7501 gaagtaggtg agatagaccg cgttgttcac gtgccccatg gcgtcgaggt cgcggaaccg
     7561 gacctcgacg gggacgacgc acgggaactc gggaagtcgg acgtcgcgca tccggatcga
     7621 ctctagcacc ccgcttcggg tgagaatacg ggtccggtgg cacgctcccc gaacctcgac
     7681 gacgacctcg actccctcct cgatcgcgcc ctgcgcggag agggggagat cgacgagagc
     7741 ctgcgcgcgc gcctctcgca ggcgttcccc gcgctgaggg tcccgcttcc gcccgccgaa
     7801 gggcgcgccg cggcgccgaa gacgccggcc gctcctcatg ccgccccgca cgcggcggag
     7861 tcgaccgggc cgatcgaggc tcccggggcg aacgcgccga gcttcgccga ggcgctcccc
     7921 ctcggcatcg tcctttccga cgcctccggc ctcgtccgga gcgcgaatgc gatggcgcgc
     7981 cggatcttcg gcgccgaggc ggtggcgctc gaagggcggc gcgtcatcga gctcttcgag
     8041 gcggtcgagc gggcccagct cgccggactc ctcgaggacg ccttccgcgg gcgtccgacg
     8101 cgcgaggtcg cgctcaacgc cgcgaacccg gcgggagcgg tcgccgttcg cgtctcggcg
     8161 gcggggcggt tcggcccgga ccgaaagctg acgcaggtcg tctggacggt gcgggaggcg
     8221 ggcgtgaacc cggccgcgct ggaggtccac gtccacagcg gcaagcagga gctcctcgct
     8281 gagctcggca tggagctggg gcgcgaggtg aacccgctcc tcgttcgcct cacggaatct
     8341 ctcgtgtcgg tccagcgcgt cctctcctcc ggcggcgacg agccgcccgc ggagcgcgag
     8401 gcgatccgcg tgaagatcgc cgacgcgctt tcggggctcc tccgcctctc cgaggtcgtc
     8461 acggagctcg agcgcttctc ggcgacggcg cccctgaaga tcgaggccgt cgacccgtcg
     8521 gagatcctgg agcgggcgca gaagctcctc ggccgccggc tcaagggggc gaagctccgg
     8581 gtgcggaacg acatggacga gccggcgccg aaggtcctcg ccgacgcgcc ccggacggtc
     8641 gagatcttcg tcaacctcct ccggaacgcg cggaacgcga tccttcgccg cttcgaaggg
     8701 gccgatccgg aggcgagcgc ggcgatgaag cgcctcgtcg tcgtccaggc gttcgtccgc
     8761 gactcctacg tcgccctcgt cttcacgaac aacggcgtgc cggtcccgca ggcggaccac
     8821 gagcggatct tcctcccctc gttccaggcc cgcgacccgg agcggagcgg cctcggcctc
     8881 cccgagacgg cggccctcat caaacagatg gggggcgcga tccgctgcga gccgctcggg
     8941 gagcagggga cccgcttcat cctgacgctg ccgaaggcgt cgtagcgccg gccggcccgg
     9001 cggtccgggt ccccccggaa gacgctcggc ggcgcggagg tccgctccgc gcggaggggg
     9061 atcgggggaa cccggcggtg cgggttcccc cggaagacgt cagaccccga ggaccctccg
     9121 cccttccggt gaggccatct cggcgctcca gggcgggtcc cagacgagcg agacctcggc
     9181 gtcgacgacg ccggggaccg cgaggaggcg cgcgcgcgcg tcaccggcga tcgcgggccc
     9241 catcccgcac ccgggggcgg tgagcgtcat cttcacggcg acctgcgtcc cgcgtccctt
     9301 cgccggcgaa agcgtcaggt cgtagacgag gccgagatcg acgatgttca ccgggatctc
     9361 cgggtcgtag cactgccgga gcgctgccca gacctcctgc tcggaggcgg gacccttcgg
     9421 cgcgcgcttc ggaggcggat cggccgagga gggaacgagg ccgagcgcgt ccgcgtcttc
     9481 cggtccgatc cggaagaggc cgccgacggt cggcgcctgg atggtgaacg agccgccgag
     9541 cgactggacg aggtagacgg gcactccttc gggcaggagc gccccctcgc cgctcggcac
     9601 gcgcaccgcc gcgcagtcgc gccggaggag gatcgcgggc gtccggctca cgcgggcctc
     9661 gtgcgcgggg aggagacggc cttcagtccg acgaggacga cgacgacgac ggccacctcg
     9721 agcaacgctc cggtgccgta ggggcccgcc gcgtcccacg cgaaccaggc gtgctcgccc
     9781 cagaacggtc cgacgatccg cccgagcgag gagagcgact gggccgcgcc gaggatctct
     9841 ccctgctcgg ccgcctccgt cgagcgggag aggaggcctg cgagggcggg gttcacgagc
     9901 ccgctccccg ccgccgtggt gacgaccagc gcgacgagcg cgccgtgccc cggcacgagg
     9961 acgagccccg cgaacgagag cgccaggagc gtcgagccga taccgatgag gcgcgcttca
    10021 ccgagaagag ggacgaggcg ccggatgaga ccgccctgga ccaccgcggc cgtgacgccg
    10081 atcaggacga agagccagga gaccgtcgac ggatcgtggc cgaagcggct ccgcgcgaac
    10141 tgcgcgaacg tcgcctcgaa gttcgcgaag gccgtcgtgt tcaggagcgt caccaggagg
    10201 agcgggccga cgacggggcg cgcgaaggcg ttcaggacgg cgcgcagggg gaaccagtgg
    10261 cgcggcgggc gcgcggcggc gctcgcgggg cgcgtctccg ggaggccgaa gtacgcccag
    10321 agccatgcga cgagcgagag gcccgcggcg acgaggccgg ggagggtgtg gctgaccttc
    10381 accgagaagc cgccgatggc gggcccgaag acgaacccga gcccgaacgc catgccgacg
    10441 agccccatgc cgcgcgcgcg gtcgccctgc ggcgtcacgt cggcgatgac ggcctgcgcg
    10501 gtgccgatgt tcgcgcccat gatcccggcc aggacgcgcg aggcgaggag gcccccgaac
    10561 gagccggcga acgcgaacag gagataggag aggaagctcc cgaagagcga gaagaggagg
    10621 acgggccgcc ggccgacgcg gtccgagagc cgcccgagga gcggcgcgaa gacgaactgc
    10681 atcgccgaga agctcgccat cagcaccccg aaggcgagcg gcgacgggtg gtaggcctcg
    10741 gcgtagagcg ggacgagcgg gatgacgatg ccgaagccga cgaggtcgat gaagaccgtc
    10801 aggaagacga cggcgagcgg cgagaggcgg cgggtcacgg ctctccttcg cggaccctgc
    10861 gcagaaggcg acggcccccg cgcgggggcc gtcgtcgagg cgcgggggtc agcgtcggct
    10921 caggcgaagc tcttcccgca gccgcagccg ccgccggcgt tcgggttctc gacggagaaa
    10981 ccggcgccgc ggaagtcctc gatccagtcc acggtcgcgc cgttgagctt catggcgctc
    11041 gtcttgtcga cgaggacgcg gatcccgccg acggcgagga cgtggtccgt ctccttggcg
    11101 tcgtcgaacg tgaagccgta ctggaagccg gagcatccgc cggcctggac gaagacccgc
    11161 agggccttgt cggccgcgtc gggcatcttc gcggcgaagt ccttgacctt gtcgacggcg
    11221 cggtcggtga ggctgagggc gaacgtctcc tggacggaga cggtggcggt gttgagagtc
    11281 gtggacgcca tcggttcctc ctcgaggccg gacgcaccga aggcgaacgg cctcgaacct
    11341 ttcattgtcg ctccggtcga gactgcgcgt caacgcgccc cgccccgttc accagcaaga
    11401 atcggaccat ccgccacgct acagcacaac ccctagtggt ctcccggctt cagactacga
    11461 gatgttgcgc aagggcgcgc ggtcgcatag gattcctcat ccgcccatcg agaattccac
    11521 aagggggtcc gccgtgtctt ttcccgccga tcgcaccgcc gagcaggcgt ccgtctccac
    11581 cgcttccgag cccgccgcgt cccgctccgc gctccgcggc ctcacgttcc ggcgcctcta
    11641 cacggacggc gtcacgcacc cgttcgacgc gatcgagtgg gagctccgga cggccgcgat
    11701 cacgaacgag aagggcgagg tcttcttcga gcagaaagac gtcgaggtcc cgaaagcgtg
    11761 gtcgatgacg gcgacgaaca tcgtcacgca gaagtacttt cacgggaagg tcgggacgcc
    11821 ggagcgggag cgctccgtga ggcagctcat cgggcgggtc gtcgagacga tcacgggata
    11881 cgggtcgaag gggggctact tccggaccgc ggcggaccgg gacgccttcc gcgacgagct
    11941 cgcggcgatc ctcgtcaacc aggccgcgag cttcaactct cccgtctggt tcaacgtggg
    12001 cgtcgaggag aagccgcagg cctcggcctg cttcatcaac gccgtgggcg actcgatggg
    12061 ctcgatcctc gacctcgcga agaccgaggg gatgctcttc aagttcggct cgggcaccgg
    12121 ctcgaacctc tcctcgctcc gctcctcgac cgagtgcctc tcctcgggcg gcatcgcctc
    12181 gggccccgtc tcgttcatga agggcttcga cagcttcgcc ggggcgatca agtcgggcgg
    12241 caagacgcgc cgcgccgcga agatggtcat cctgaacgtc gaccaccccg acgtcctcga
    12301 gttcatcgac tgcaaggcga aggaggagcg gaaggcgtgg gacctcatcg aggcgggctg
    12361 ggacggctcg ttcggcggcg aggtctacaa caacgtcctc ttccagaacg cgaaccactc
    12421 cgtccgcgtg acggacgagt tcatgaaggc ggtcgaggag gggcgcgagt acgcctgcca
    12481 cgccgtgacg acgggcgagg agctcctcaa gctcgacgcg cgagccgtcc tccgccggat
    12541 ggccgattcg gcctggatct gcggcgaccc cggcatccag tacgacacga cgatcaaccg
    12601 ctggaacccc tgcaagtcga cgcaccggat caacgcgtcg aacccctgct cggagtacat
    12661 gttcgtcgac gactccgcct gcaacctcgc ctcgctgaac ctgatgaagt tcgcgaagga
    12721 ggacggcggc ctcgacgtgg agaagttccg ccacgccgtc gacgtcgtct tcgcggcgca
    12781 ggagatgctc gtcgacgagg cctcctaccc gacgccggcc atcgagaaga actcccacga
    12841 cttccgcccg atcggcctcg gctacgcgaa cctcggggcg ctcctgatgt acaacggcct
    12901 cccctacgac ggcgacgacg ggcgggcgta cgcggcggcc gtcacgagcc tgatgcacgg
    12961 gcaggcgttc ctcacctcgt cccgcatcgc ctccgagatc ggcaccttcc ggatgtacga
    13021 gccgaaccgc gatgccttcc tcggcgtcat cgggatgcac cgggaggcgg cggcgcaggt
    13081 cccgcggacg ggcgtcccga aggagctcta cgaggcgcag ctcggcgtgt gggacctgac
    13141 gctcgagtcg ggccgccagc acggctaccg caacgcgcag gcgaccgtcc tcgccccgac
    13201 cgggacgatc ggcttcatga tggactgcga cacgacgggc gtggagcccg acctcgccct
    13261 cgtgaagtac aagaagctcg tcggcggcgg gacgatcaag atcgtcaaca acaccgtccc
    13321 tcacgcgctc gcgcgcctcg gctactccga gccgcaggtg aacgagatcg tcgcgtacgt
    13381 cgacgagaag gggacgatcg agggcgcgcc gcacctctcc gccgcgcacc tccccgtgtt
    13441 cgactgcgcc ttcaaggcgc gcggcgggac gcgctccatc gacgcgatgg ggcacgtgcg
    13501 gatgatggcc gccgtccagc ccttcctctc gggcgcgatc tcgaagaccg tgaacctgcc
    13561 gcccgacgcc accgtcgagg acatcacgaa cgtctacgtc gagggctgga agctcggcct
    13621 gaaggcgatc gcggtctatc gcgacggatg caagcggacg cagccgctca acacggcggc
    13681 gtcgaagagc gacgacgccg tgaaggggac gaagggggcc gcggcggccc cggcgcccgc
    13741 tccggccgcg acggccgggc cgcgacggaa gaagctcccg gacgagcggc gcgcgctcac
    13801 gcacaagttc tccgtcggcg ggcacgaggg atacatcacc gtcggcctct acgaggacgg
    13861 ccagccgggc gagatcttcc tgacgatggc gaaggaaggc tccacgatct cgggcctgat
    13921 ggacgccttc gcgacggcga tctcgctgac cctccagtac ggcgtcccgc tcgaggcgct
    13981 cgtcgagaag ttcagccaca tgcgcttcga gccggccggc tacacgaaga accccgagat
    14041 cccgatcgcc aagagcctcg tcgactacat cttccggtac ctcgcgtcgc gcttcctgac
    14101 ggccgacctg cgcgcgcgcg ccggcgtcat ctcgcgcgag gagaacggca acggcaacgg
    14161 ccacgccctc gcggccatcc ccgtcccgcc cgcgcagaag gcggccgcgg cctccgcgac
    14221 gacgctcgcc ttcgagaaca gcgccgacgc cccgagctgc caccagtgcg ggcacctcat
    14281 ggtgcgcaac ggcgcctgct acaagtgcct gaactgcggg gagacgagcg gctgcagctg
    14341 atgaatggat agggggaccc gggttccgcc cgggtccccc tggcccgctg cgcgggcgac
    14401 ccccccgcgg cggagaggcg gcggccttcg gccgcgacgg agccttcgga atcgtggcgg
    14461 ggaaggttcc gcccgggtcc ttctcgtttg ctcggcgagc gaccccctgg tggcgggaag
    14521 gcggccgaac gccgggttcg ctcgtcctag gacgacgagg gcggcgattc caggtggcga
    14581 atggccgtga agctgtagga gaccgcctgt gggcccacga cttcgttctc gatgctcatg
    14641 ggctcggtga agagggtggc tcccggctcc aggacgaccg cgtcggcacc gacggccgcc
    14701 gccttctccc ggaccttgcc gagaatcgcg gcgctgctgt ggcggcccga gatccacacc
    14761 tcgatcgttc cgagggactg gtgaggagcc ttgggaaggg agcggaggac cgcgacctcg
    14821 gccggagaga ccgatggccg aacgccggga gcgcggtcct cgcccgggtg ccaggtcgtg
    14881 gagacgcagc cgaagagcag gaatagagag aatcccgtgg cgagcagccc gatccgcgtg
    14941 tgtcctctca tgaggatacc tcctggatcg gacaagcaat gaacatgccc gcggggccgc
    15001 atcgtccgga ggacagccgt cgcggcgtcc acgaggccgg gacggcgcgg cgcgtccggt
    15061 ccccggcggc gcctacttct tcttgccgaa gagggcgtcg aaggcggagc gggcctggtc
    15121 gggggtgtcg gtcgcctttc ggccggtcag gtcgagcgtg accgcgggct cgtagaacga
    15181 gcactcgttt gcgcgggact tcgcggagat cggcgccggg atcggctggc ggcactggaa
    15241 gggggcgccg ccgtcgaagt gacggcactg ggcgcaggcg tggaggggcg cgcgacactt
    15301 gacgcagagc gcggcgaggg cgaccgcggg gtcgttcttc tcgtggcacg tgcggcagcg
    15361 gaagacctct tcgctgggcc ggccggcgct ccgtcctcgc ggcgccccct cgagccgtgc
    15421 ggggcggtcg tcgaaggggc cgccgctccg gggcgtcgac ggggcgttgt cctggtagcc
    15481 gcgctggcgg tacttccggt ccatggaggt cacctcgggc cggagggtgc cgcaccgtcg
    15541 gggcgcgcgt caacgccgcg aggtatcgtc ccggtgctgg aaggagagcc cgcagatgaa
    15601 gctgatcctc agggtgctcg tgaacgcgct cgcgctcggg gcggcggcgt acctcgtccc
    15661 gggaatccac gccggaggcg tcgagtcggt cctcctgatc gcgctcgtct tcggcgtcct
    15721 gaacgccctg gtccgtccct tcctcaagct cctctcctgc ccgctcctcg tcctgacgct
    15781 cggcctcttc accctcgtcc tgaacgcggc catgctcatg ctcacggcct ggctcggcca
    15841 gaagctcggg atcgacttcc gcgtcgacgg cttctggccc gcgttcctcg gcgcgctcgt
    15901 cgtctcgatc gtctccgtcg ttctctcctg ggtcctgatc gacgagaaga aggggtgagg
    15961 gcttgacctc cgggttatcg ttctcggcga cgtgatgaag aacggccttc tcctctcgtt
    16021 tacggcgacc ggcggcgtct attcgccgtt ccgcggaggg gtgggcgtgt aggaacggcc
    16081 acagagaagg gcatcccgaa gcccccctcc gccggatcgg tggagggggg cttcttcgtt
    16141 ttcggcgaag ccgagaagga ggacgcgatg gcggagcgga acgaggcgga ggggcgcgag
    16201 ggagcgtgcg agcagcgcaa ccggcgcgcc ggctaccagg ggcgcgagag ctccttcggc
    16261 gcccgcgcgg cggcgcgctg cgggcggccg gtggctctcc cgagcttcga ggcgctcctc
    16321 gatgccctcg tcgcgggggc cgtcgacgag gcgctcctcc cggcggtcga ccgggtcatc
    16381 gggccggtcg tcgaggcgct cggcccgctc ggcgcggccg tcgaccgcgg cgcgcgcttc
    16441 gcggtcctcg gcgaggtgga ggtgccgatg cgcctcgtcc tcgcggcgcc gcgcggcgtc
    16501 gccctcgagg agatcgagga gctgcactcc cagccccccg tcctccggca gtgcgcgggg
    16561 ctcctcgcgc gcctcggggc gcgtgccgtg ccgacggagg acacggcaca ggccgcggtg
    16621 aacgtcgcgc gcgcgggcgg gaggcgcggc gcggtctgct ccgaggaggc ggcgttcgag
    16681 gccggcctcg tgccgctcct cgaggacgtc tcggacgtcc ggcccaacgg gacccgcttc
    16741 tggcgggtcg cccggtcgga cgacgccctc ctcggagagt cccccctgcg cgtcctgcgg
    16801 gtcacgacgg agcgcctcgg ggcgcacctc cggcaggccg gcgtcgaggt cctcgcctcg
    16861 ggcccggggt ccggcttcgc gctcgtcggc gatgcgcagg tcgcggcggc cgcggccgcg
    16921 gtgcgcgagg cgaaggcgaa gggcgcgacg tggctcggca cgcctccgga cctcgtgcgg
    16981 cggccccggg tcgaggcgct cccgcgtgcc accgaggtcc gccccccgac ggtcgtgacg
    17041 gtcggcggct tccgggtcgg cggcggggcg cgggccgtca tcgcgggccc gtgcaccgtc
    17101 gagtccgagg agcagatcgg gcgcctcgcg cgagcggtgg cgcgggccgg ggccaccgcg
    17161 ctccgaggcg gcgtcttcaa gccgcgcacc tcgccctact ccttccaggg gcacgggctc
    17221 cacgccctcc gctggctgaa ggaggcgggc gagagcgtcg gcctcccggt cgtcacggaa
    17281 gtcatggcgc cggcgcaggt cgggccggtc gctgcggtcg cggacgtcct ccagatcggc
    17341 gcgcggaact gccagaacta cgaccttctc aaggaagccg gggccgcggg gcggccgatc
    17401 ctcctcaagc gcggcttcgg cacgacggtc gacgagtggc tcgcgtcggc cgagtacctc
    17461 ctcgacgcgg gcggcgccga ggtgatgctc tgcgagcgcg gcatccggac gttcgagcac
    17521 gccacgcgcg gcactctcga cctcgggggg ctcctcgcgg cgcggcagct gacgcacctg
    17581 ccgctcctcg ccgacccgtc gcactcctcg gggcgccggg agctcgtccc gggcctctcg
    17641 cgcgcggcgt ggggcgcggg cgtcgacggc ctcatcgtgg aggtccacga cgcccccgag
    17701 tcggcctggt gcgacgggcc gcaggcgctc ctcccggagg acctcgagcg gctctgcgac
    17761 gagctcgggc tcctgccggg cgcgaccccc tcgatcgagc gcgtgcggac cgccatcgac
    17821 gccgtcgacc gcgagatcgc gcgcctcgtc gcccgccgcc tcgagctctg ccggagagtc
    17881 gccgaggcca agcgcgccgc gggcgttccg ctccgcgacg ccgcccggga ggagatggtg
    17941 acggcgcgct acctcgagac gcccgggatg gacgaaggct cggcgcggcg cctcgccgag
    18001 tcgctcatcg cgatggggct gacggtcgag gggtggtccg ccgagggggc ggcgtgagga
    18061 gggtcactcc ccgatgatct tgatcaggac ccgcttcgcg cgccgcccgt cccactctcc
    18121 gtagaagacc tgctcccagg ggccgaggtc gagccgcccg ccggtgacgg cgaggacggc
    18181 ctcgcgcccc atgagctggc gcttcacgtg ggcgtccgcg ttctcctcgc ccgtgtcgtt
    18241 gtgccgccag gagcggaccg gctcgtgcgg gacgagcgcc tcgaagaagc gctcgaagtc
    18301 ggcgaggagg ccgcgctcgt cgtcgttcac gaagaccgac gccgtgatgt gcatcgggtt
    18361 gacgaggcag agcccctccg agatcccgct ctcgcggacc gcggcctcga cctcgggcgt
    18421 cacgtttacg aagccgatcc gcttcgggag gtggaacgtc agctctcttc gatacgaccg
    18481 catgctcgcc tccgtgcggc ggcaaggcta gcctgcggcg aagggcggcg cgagccgggc
    18541 cttccgtgcg agatgccccc tccctcgcgg gcgccggttg cgatatcgtc tccgccatga
    18601 cggaagccgt cgaggaacgc cgacttttcg ccgactttct gcggcggcgc gggctgaaga
    18661 cgacccgcga gcggaccgcg ctgttcgaag agatcttcgc cacgcaccac cacttcgacg
    18721 ccgacgacct cgtcgtccgg ctgcggggca aggggaagaa ggtgagccgg gcgacggtct
    18781 accggacgct cgacctcctc tacgactgcg gcctcgtctc ccgcgtgcgg ctgaacgacg
    18841 agaagtaccg atacgagcgc ctcctcgtcg gcgagcacca cgaccacctc gtctgcacgt
    18901 cctgcggcgc cgtcatcgag ttcgtcgacc cgcgcatcga ggagctgcag gagacggtct
    18961 gcaagaagta cggcttcgcg ccgacgaccc actcgcacca gattcgcggc gtctgctccg
    19021 actgccaggc ccgcgagggg acgtcccggg gagcttcacc ggcgtagagg ggccgtatgg
    19081 cccccgcgat cgtctggttc cgccgaaacc tccgcctcga cgacaacgtc cccctggacg
    19141 cggcgctccg cgggcacgag acgatcgtcc ccgtcctcgt cctcgacgac cattaccggg
    19201 acgaggactt ctctccgccg aggctccggt tcctcgcgga gagcctccgg gagctcgagg
    19261 cggagctcgc cgcgcggggc tcgcgcctcc tcgtgcggaa cggccccgcg ggggatgctc
    19321 tggcggcgct cgcgcgcgag accggggccg aggccgtcta cgggcacgac gaccacgaac
    19381 cgcacgggcg ggcgctcgcg cgggaggtcg atgcggccct ggcgtggcag gggacgcggc
    19441 tgcgccttct ccccgacctc cacctcgttt ccccggggtc gcttcggacg gactccggga
    19501 gaccgtatgc ggtcttcacg ccgttcctga agcgttggcg cgaggccgac aaggccgccc
    19561 cccgcccgac gccgccgcgc gtcccgaccc ccgacgcggt cctcgacacg tccttcccgt
    19621 cgatcccgct ctcgaggccc cgccgccttc gcgaggtggg ggcgcccgcg aacccgcccg
    19681 gcggggcgcg agaggcccga catctctggg acgggttccg cgagtccgct ctcgtccggt
    19741 acgaaggggg ccgcgacgtg ccggccgcct ccgggacgtc gcgcctctcg ccgcacctgc
    19801 ggttcgggac gatcggcatc cggcggctcc tcgcggaggc acgcgccgcc tggaaggacg
    19861 cggacgcgcc ggggcgggcg tcgatcgacg tcttcgtcgc cgagctcgcc tggcgggagt
    19921 tctacgcctc gatcctcgcg gagaatccgc gcgtcctgac ggagagcttc cggccggagt
    19981 tcgacggctt cccctggtcg gccggcgagg aagcggagga gcgcttcgcg gcgtggcggg
    20041 acggacggac cggctacccg atcgtcgacg cggggatgcg ccagctcgcg cacgaggggt
    20101 ggatgcacaa ccgcgtccgg atgatcgtgg cgagcttcct cacgaagcac ctcctcgtcg
    20161 actggcgtcg cggcgaggcg ctcttccgcg cgcgcctggc cgacggcgac ccggcctcga
    20221 acaacggcgg atggcagtgg tcggccggct gcgggaccga cgcgcagccc ttcttccgga
    20281 tcttcaaccc ggtgctgcag ggggagcgct tcgacccgga cggcgcctac gtgaagcgct
    20341 gggtgccgga gctcgcggcc tggaagggcg cgggccggaa tctccacgcc ccctggcgcg
    20401 ccccgtctcc gcccgcggac tacccggcgc cgatcgtcga ccacgccacg gcccgcgcgc
    20461 gggcgcttgc cgccttcgac gcgatcaggg gcgcggccgg gtcgccccgc cgctgacggg
    20521 gaggggatcg atcgcggcgc cgtggaggac ggcgtacgcc gcctcgaaga agaggacgcg
    20581 cttgacgtag tcgcgcggct cgaagagagg catcgcctcg aggaactcgt cggccgcgag
    20641 ggcgggagcc tggcgccgcc agcgtctcgg ccgccccggg ccggcgttgt agccggcgag
    20701 cgcggcgatc cggtcgccgt cgaactcgcg gagcatcatc gcgaggtagc gcgccccgag
    20761 cgcgacgttg acctccgggt ccttcaggtc ggcacggccc cggcgtcctt cgcgccggag
    20821 gaggtcccgg cccgttccgg gcatcacctg catcagcccc gtggccccgg cgtgagagcg
    20881 tgcgcccgtc tggaagaggc tctcctgccg gatgacgccg tagacgagcg cgggggggag
    20941 gccgttcgcg gtcgcctcgc ggacgatcag cgcctcctgc cggaaggggt agtacgcccg
    21001 gcgcaccgcg aggggaagag ccccctcgtc gggcgtgccg agctcgggcc agcgggactt
    21061 caggatcccg acggcgcggc ggaactcgaa cctctcggag gcacaggccg cgaggaagag
    21121 cgggtcggcc gatcgctcct cctcggccgc gtcctcggcc agggaggcga gcccgacggc
    21181 gaggagctcc cgcgagggaa gtgacggggc ctcggcgcgt agcgcgtgcg gcagaggcgg
    21241 gccgggcgga tcgagaggga gggcggcgcg gaccgggatg ccgagccggg cgccggccca
    21301 gcgcgcgtag aggtcgggga cggcggtgga gacgagcgag gcctggaggg cccgcgcggc
    21361 ggcgcccttc ccggtcgcct cgagggagcg tgccgcccag tagatcgccc gccgacgcac
    21421 cgccacgtcg ccgtagagcg cggcctgttc ctcgagggct gccgcggcgg cgcggtgcgc
    21481 ctcgggcgtg cgcgtgagcg tcgggaggaa cgcggcgcgg aagagctcct cggcgccgac
    21541 ggtggtcgcc gggtcgatcg cgacgagcgc ggggaggaac cggcgcgccg cctcgagctg
    21601 gccgaagcgg gcggcggccc gaacggcctc tccaagcagg cgcctccggt ccatggcggc
    21661 gagggggcgt gcgaggaggg cctcggaccg cgccgcgagc gacgcccagg cccggagcgt
    21721 ctcggcgtcg ggaggggcgg gaggcgcccc cgggacggcg ggcgcggggc gcggggcgcg
    21781 gcgatgccgc ggggccggcg ttcggcgcgt cggctcgtcg aggagccgga attcggcggc
    21841 gaggagaacg acctcgatcc ggaggcgttc gccgtcgtcc cggccggcga gcgcggaggg
    21901 cttcaccagg gcatcgcggg cggcgcgggc ccggcccgcc gcgaggagga ggtccgccgt
    21961 gtcgagccgg taccccggca ggcgggccag cggcgcggcg aggtccgccg cggcggacgg
    22021 gctccgtcgg gcgagcgccc gggcgcggac gagcttcagc tccggtgcgg ctcgggagag
    22081 gagccgggag aggagcgcgt gatcggcggg ctcgaagagg tcgggtgcgg tctccggggc
    22141 gtcggggaac gtccgaagga ggttcgccat ctggtcgacc gcctccgctg gggtcctcgc
    22201 gaggcgggtg cggaccagcg ccgcggagat ccggccgcgc ggcgtccccg ggccgagccg
    22261 ctccctggcg gcgagggcgt cgaggaccgc ccgcctctcg gccggcgtcc gggcggcccg
    22321 ggcggcggag acgagcgcgg cgcgagcgag cggctcggcg cgggtaccgg ccgagaagcc
    22381 gaggagcctc gagacgagga gccccgagcc ggcgcgcgac gccgggtccg cagccgcctc
    22441 gagagtgaga aagggggcga gatccggaag gccgagcggc gtgagatccg ggaagggccc
    22501 ggcgacggag cccccctcca tggcgatcac ggcgcgggcg gcggccgaac ggaccgcgaa
    22561 gagccccgtc tccggcgctc ccgcgaaggc agaggcgtcg ccttcccggc cggcgcgccg
    22621 cgccgtcgcg aggcgctcct ccggcgatgc cgcgccgagg gcgcccgagg cgagcgccgc
    22681 agcgacgaga aggagcgggg cgagccggtg gaaggtcagg agggaatcgg accgagcgct
    22741 tccggctttc cctcggcgag gatcctgacg agctcttcct ggaagaagtc cgaggtcgcc
    22801 cggacgtgct ccggggtccg ctcggcatag acctgacgac tccgctcgat gtcgtccttc
    22861 aggcgctcgt agaggtcgca ggcgacgcgg ccctcggcca cttttcgctc gttgtagagc
    22921 ttgatctccg agacgaggag gcgggcgatg cggcgggcgt cgtcgatggc ccgctgcggg
    22981 tcgtccccgc cgcgcggggc gcgggaccgc ggaacgaatc cgggcggagg cgcgaggggg
    23041 gaagcggccg acccggccgg cacgtcgagg cctcccgggg gagccgccgc ggcgacagcc
    23101 gcaggcggcg ccggcggacg gggtgtcggc gcgggcgtcg gcggcgccgg ggccggcggc
    23161 ggcggcggcg tgacggtcct gatctgctcg aaggccatcg tcaggtcgcc ccggttcggc
    23221 ggggcggcgg gcgtcggcgt ggccagacgg ggcgtcagct cgggcgcgga ggagccggga
    23281 cgcggcgtcg cgagcccgag agcggcggcg ctccgcgctc ccgaggccgt gaggatgtcg
    23341 acgccggaga acggatcctt cttcttcgtg gtcgccgatt cctcgagcgc ccgcagcgcc
    23401 tcgccagcgt cggtgatccg gcgcgtcttc cgcgcggagc tcgggtcggt ctgctcgaag
    23461 accgagcgtc ccgggaggtc gcccgtgatc gagagcgagg ccgacgccgg cggcgcttcg
    23521 ggaggggccg gcgccggtgc gggcggcgca ggaggggccg tcggggcggc catcgcggcg
    23581 gacacgggcg gggcgggagc tggagccgga gccggctcgg attcgggagc cggctcggcg
    23641 gcggccgcaa tcgccggctg gaaggtccgg acctcctctc cacgcggcgt gagcgccgga
    23701 gaggggatct tcttgcgcgc cgcgaggaga tcgaccgaga ggcctgtgac gaacgtcagg
    23761 acctcgatgg ccgactcgtt caggcgcccc tcttcgccgg ggagctcgtc ggcgcagagg
    23821 aggccggcga tccggtcgcg gatcaccatc gggacgagca gggtccgggc cggcggatgg
    23881 cctccgaggg cgcggcgaag cgccccgctc tccggcggcg gaaccgcgac catcggcttc
    23941 tccgcatgca ggcacgcctc cacgaacggg tcgtctcccg ccgccaggtc gagccccttg
    24001 acggaatcgt cccgtccgcc ggaggcgtcg tacccgagcg ccttccagcc cgtcagacgg
    24061 tcgttgcgga ggacgaggag cgcggcgcgc gaggagtgga cctgcgcctc cgtcaggaag
    24121 tgcgtcagga cgtccacctg cgtccgggag ccctcgatcg ccgcgatcga gctcttcacg
    24181 aggccccagt cgggagccgc gggggggccg gggacggtca cggtctcgac gacccgctcg
    24241 acgaccttct cgacgacgcg ctccggcgcc ggggccggcg ggacggcgtc ctcgaggagc
    24301 gtggcgacga ggtcctcggg gaacaggtgc cgtgcctggg cggggatgct ctccgaggcc
    24361 ttcgagatca gctggtcgat ctcgttgcgg gtatccgcga gctgggactt ccaggtcgcg
    24421 aggtggtcct cgaggagggt cgaaagcgac tcgatgaacc tgcgattggc ggcggggtcg
    24481 acgggcatga cacgcctctc ctggatgcct tcggggtcgc gagccggcat tatagacgcg
    24541 cgttcccgac ggtcccgccc cgggcgggcc gcggttgacg gcgccgggac ggactcgtag
    24601 actcgcaggt ccgcctcggt ccggccgact cggccgcgcc actccgggcc cgaaaggggt
    24661 gactctcagt tgcctttgat ccacgtcaag gaggacgagt ccttcgagaa cgcgctgcgc
    24721 cgcttcaagc ggaagtgcga gaagtcgggg atcctgtcgg agctcaagaa gcgccagcac
    24781 tacgaga
//
LOCUS       DAOTEZ010000007        23921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_1397_length_23921_cov_6.671583, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000007 DAOTEZ010000000
VERSION     DAOTEZ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 23921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23921
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1397_length_23921_cov_6.671583"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="P5164_00770"
     CDS             <1..321
                     /locus_tag="P5164_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010628601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRY42449.1"
                     /translation="LGHFLGIQVHDVAGRQADRVGTLAPPPAEYPALRTTRTIEAGML
                     FTIEPGLYFIPMLLETHREGANRADVDWAIVDRLTPFGGIRIEDDVWVGEDGVRNLTR
                     PELP"
     gene            422..1483
                     /locus_tag="P5164_00775"
     CDS             422..1483
                     /locus_tag="P5164_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HRY42450.1"
                     /translation="MRLSGESPREAGFAQPAEWAAHEACWLAWPSHEELWKEALGPVQ
                     GAFVRFARAIADPGPEGPRGERLEVLAPTAGRAAEARARLEGLGARVHEIPFGDIWMR
                     DIAPVFVTSEAGEVAAASFVFNGWGNKYDLPGDREVSKRVAEASGLRAFRDELVLEGG
                     SVEPDGEGTLLTTRQCLLNPNRNPGLSQAALERRLRDGLGAESVLWLGDGLINDHTDG
                     HVDTVARFVAPGVAVCMEPSGGGDPNAVALKEILRDLASMRDAKGRRLEVFTVPSPGV
                     VTDPDGELMAASYVNFYVANTTVTVPVYGTPNDAPALKRIESLFPGRRVHDVPARELL
                     LGGGAFHCISQQQPKGGRG"
     gene            1480..2349
                     /gene="aguB"
                     /locus_tag="P5164_00780"
     CDS             1480..2349
                     /gene="aguB"
                     /locus_tag="P5164_00780"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HRY42451.1"
                     /translation="MTSETLSIGIVQCALGGSREENADRVLGLVREAARLGAKLVVTP
                     ELLEGPYFCREEKDAFFEWARPAEGNATLARFQTLAKELGVVLPFSFFEKAGNAYYNT
                     LAMVDADGTLLGTYRKSHIPDGPGYEEKFYFRPGDTGFKVFDTKAGKVGGAVCWDQWY
                     PECARALALLGADVLVYPTAIGSEPANPALDTRDRWRRAMVGHAVSNVIPVAASNRIG
                     EEDGQLFYGSSFVAGTDGSFLAEMGRDETGVRVVTVDLAAIRRERASWGFFRDRRPEL
                     YGVLTTADGRQKV"
     gene            complement(2388..3089)
                     /locus_tag="P5164_00785"
     CDS             complement(2388..3089)
                     /locus_tag="P5164_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRY42452.1"
                     /translation="MITIRKSDERGRFDHGWLDTRHTFSFADYWDPEHVHFRSLRVIN
                     EDRVVPGAGFGTHGHNDMEILSYVLSGSLAHEDSLGNGSAIVPGDVQYMSAGTGVRHS
                     EFNASGSEPVHFLQIWIVPDRRGYAPRYGQKRFEEEEKRGRLRLLASETGEAGSIAIR
                     QDVRLFSTILREGESVPLEIAEGRHLWVQVARGEADVNGHRLEAGDGLAASDETSFTL
                     RGAGAEAELLVFDLA"
     gene            3284..4906
                     /locus_tag="P5164_00790"
     CDS             3284..4906
                     /locus_tag="P5164_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRY42453.1"
                     /translation="MRADDLTLDALFDADPKGGIYRFAGERAILLDTAALGLLRKELI
                     ETLSLSVARGVLYRFGWAHGWRTAEAMRDAFEWDGPTEWIHAGGRLHRLKGLVTFRPV
                     PRAPNEPPRHFSQAFWDESYEAEQHLLHFGRSDEPVCWTLTGFASGYLSACHGRSIRV
                     VEEECVGKGDPACRMAGEPEEDWDEATRKALSGLSERGLEGELGRVRDALRATEQKLK
                     ARRNELTRLKVEEPHPGVVAQSPGMRRAFDLASRVARVDSTILLTGESGSGKEVLARV
                     IHERSPRAAGPFVAINGAALPESLLESELFGHARGAFSGAASDRAGLFEAANGGTLFL
                     DEIGEVPLGLQAKLLRALQEREVRRVGENRSRKVDVRVVAATNRDLGKDVETGHFRRD
                     LYYRLRVIEIPIPPLRERPEDVLPLARALLLDAARRTGAQVAGFTPEAARLLQRHRWP
                     GNVRELANAVERAAVLADGARVGAEDLPDEIVRPARGGGAEGETLAEVEKAHVLFVLG
                     EEDGHREKAARRLGIGVATLYRKLKEWGDPGR"
     gene            complement(4911..6239)
                     /locus_tag="P5164_00795"
     CDS             complement(4911..6239)
                     /locus_tag="P5164_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HRY42454.1"
                     /translation="MSADELPIAAVRGVRKTYRDEAGRERVILDRVDFEVRRGEVVAV
                     LGPSGCGKSTLLRILIGLIEPTAGEVLAHGAPIRGIHPGAALVFQSFALFPWLSVEEN
                     VRIGLTGRGVPDPESAARVRDVVARVGLSGHERAMPRELSGGMKQRVGIARALVSGPE
                     LLCMDEPFSALDVLTAELLRAEVYRLWSDGGTGLSSVLLVTHLIEEAVTLADRIVVLG
                     ANPGTVRREIRNTLPHPRDPRHPEFFRMVDRIHDAVARVHLPEEEEAPAPAAPGRPGP
                     GPFVPLPPATVGEMLGLLEILSDHGGEMDLFEVDAMTEWDFGRTIAVVKAAELLDLVD
                     TPKNRVLLTDAGRGVVAAAPREKPFLFRRQLLALGTFATVVRGLAGREDAAAPGEEVR
                     EMLADRLPAEATGALFETLVNWGRFAQLLDYDAAGDELSLFGGRAADAEV"
     gene            complement(6236..7978)
                     /locus_tag="P5164_00800"
     CDS             complement(6236..7978)
                     /locus_tag="P5164_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY42455.1"
                     /translation="MTAAPVGVAPSPRTPGSAAAGRAIDLLLLAALGASIAGIVALAE
                     EWGAPLREAAPIDLVPSALPGYTLLSLSRGLAAYVLSLLFTLVVGTAAARSRRAERVL
                     IPLLDIGQGIPVLGFLPGLVLGMVALFPRTNVGLELACVVMIFTGQVWNMAFSFYGSL
                     KGVPAEMYEVARLNGFSRWKTFRVVELPSAVIGLVWNSMMSMAGGWFFLTVNEAFVLG
                     GRDFRLPGVGSYMAVAIEKGDTRAMAWAVVAMTAMIVAVDQLLWRPLVVWSQRFRVEE
                     SGSGEPPASWVVSLVGRSRLLRRATRSLRKARAAARARAAGSGPARARGAPRRALALR
                     AAAILGGGLAVAAALWGTVRLGGLLVRVPVREWGLLALALGATALRAAGALAVATAVM
                     VPLGVLLGRSREWSRRLQPVVQIVASFPAPMLYPLVTAGLLALRVPFAVVAAVLMGLG
                     AQWYVLFNVLAGASAIPGDLEEASAVYGVTGLRRFATLDLPSVFPFLVTGLVTAAGGA
                     WNASIVAETLAFRGTELSTFGLGSLITRATHDGNFPLLAAGVLTMSLALVLLNRTVWR
                     RLYGVAATRYALNR"
     gene            8085..8459
                     /locus_tag="P5164_00805"
     CDS             8085..8459
                     /locus_tag="P5164_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42456.1"
                     /translation="MFERHAEPLLSGSGFLRRQLLHAGASGLLVGAALAIGVLGYHLL
                     EGLPWVDALLDASMILGGMGPVDPIRTTGGKLFASAYALFSGLVFLAAAGVVFAPAFH
                     RFLHRFHLEADDDPGDETRGER"
     gene            8456..8917
                     /locus_tag="P5164_00810"
     CDS             8456..8917
                     /locus_tag="P5164_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HRY42457.1"
                     /translation="MSPGRRGRRAVATLEGLKGALVLLAGLGAFALVHRDAQAFAEHV
                     VRHFHLNPAGHAPRSFLEAADHLDDARLRWLAAGAFSYALLRLLEGWGLFREREWAEW
                     LGALSAALYVPFEVAAFVSRPSPLRGALLLLNLAVVTYLAGLLLERRRRRP"
     gene            9035..9130
                     /locus_tag="P5164_00815"
     CDS             9035..9130
                     /locus_tag="P5164_00815"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42458.1"
                     /translation="MLRKQAEEAVRELPGVEEVEDRLEVDGPARS"
     gene            9111..10109
                     /locus_tag="P5164_00820"
     CDS             9111..10109
                     /locus_tag="P5164_00820"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY42459.1"
                     /translation="MDRPGADAPADAGRSGAYAPAPGRPAGVAELERLLADPRVREKI
                     AAIAREEAAPEEESAPTRWQRFSAFVAGASSALVVVLAFLVPSIEEQWDRFQARRVVQ
                     KHVDLGRAFVAEGKYKLAEESFGRAFELSENKRLDVDEERLEAKVRAVDSDPDWGAAN
                     PEGLEESDFLYLLKLQEGAGHVPERAATLNGYGTFLASAKRWREAEERLREAARLDPR
                     SPAPLVGLGNLLRDRGRAPEAESFYRRALRLDGRNGWIHYDLALLLGETGRPADATAE
                     LERAAACDPSEPELLRALAEQLERTSRKEEARAVRGRLLALDPPGGEEGRRAGARG"
     gene            complement(10159..12321)
                     /locus_tag="P5164_00825"
     CDS             complement(10159..12321)
                     /locus_tag="P5164_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY42460.1"
                     /translation="MRSDDGVWRHLLGLARPYRGRFLVIAVLALLGTAADLVEPLVYR
                     AAVNDVAGLFVSRAGAGASSGLEDEEEAAPPANGPRAAPAAEGGAPALLVPTPVPTAV
                     PTAGNAGAATAGRPSAHRRHRRAARPGAPGPKATAAPAPPPAREPHRSDYVAPRTPEQ
                     AISTLLVAVGLLLVTGVLGYVFSLAADNRSTVLASRIEADLIQSTFGHVLRLPLAFFS
                     RRASGGLAKQIDQSDQVAPIVTAFAQEVAPEAIRMAGVFAIMVWQSGRLSIVALALLP
                     LYLLLAVRSARRLESGLDGYYEKWEEVSARIQDALASVKTVKLSGAEPREAEKLRGSA
                     TRAYDDYLARNRLSNRYLLGEVVVSHFSKALVFGYGGWLVLERQLTPGDVVMFVAYLD
                     RLYGPIDSLSSMAVTLQQHVASLRRAVRLLETAGGEEGGSALAAGPGAVEFRDVRFGY
                     TEGREVLRGLSLTLAPGTVTALAGASGAGKTTTADLLLKLYEPQSGEVLVDGQPLSRL
                     DPSTVRREIGVVAADGAVFRGSLADNIRYKRPDATDEEVRSAALAAGLGPALERLPEG
                     LQTPIGERGVGLSVGERQRLQLARVLVSRPRILVLDEATANLDVETEAEIKRSLARIE
                     PQPTTLLIAHRYATLRDADRVAVLDGGRVVEEGTPEELAAAGGWFARLASVSDDGAAR
                     VEGGACPGPADAEDGAEEEGSGEGDEEAAADDEGSVGV"
     gene            complement(12415..15600)
                     /locus_tag="P5164_00830"
     CDS             complement(12415..15600)
                     /locus_tag="P5164_00830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY42461.1"
                     /translation="MRAPIAALAALGLLAGAQASSVRGEVDLARPSVATYGERDGLPQ
                     GTIHAIAFDSRGYLWVGTQDGAARFDGRIWSPLDMPDRAVSNYVRAVVGTRDGSLWFG
                     REEGGVVRQRDGVTDVFDGAAGLPFGRVNHLLEAADGTLWAATHGGGVARFDGTRFEP
                     VGTGLPDPRVWSLLERHDASGTSRIVAACEGGVAELGDGGRFAPIDLGVSLSGFSANA
                     LLETDDGGERSLWVGTFGAGLFRVRAGSVTRFGPEEGLGSRFVTSLAATGPRGSETVW
                     VGTRDAGLYRLRGRAFEPVALGAGPLSIYALAAGGAGAPETLWVGTRLSGLHRVEPTP
                     WAPFDEDSGLPGDQVFSFLETRGPGGGPGVWIGTSRGAALLRGGQLSVSGPASALPAG
                     EVRSFAEVRGPGEASELWASLTGAGIFRREGTRWRPIEARPAFRSDDASVLLATRDEA
                     GRPVVWAGSERSGLGRFAAGRWTTLGEGDGLPARSVLSLLETRSPPGRTLWVGTRGGG
                     LAEVSGGRVARVHDRTSGLPNNVVLALAEVALPGGLRELWVGTRGGLARRSLDDPDAG
                     WSILTTESRPPLPSDVVLSIAPVPGGSAFLGTSRGVARLEPARSGGGLEVSTFGTNEG
                     LPSAACNQGSLVDGAGRVWISTAAGAAIFDPARARAAKPAPRPLVLERFVAGGAARPG
                     EGEVTLRRREADVAFEYALLAYHGQSLVRYRTQLVGREAAPTEWTTAHRREYTNLPPG
                     EYVFRAWGRDADATVSGPLEVSFRVTQPFWRRPAALVLWSLLAAALGLAVVHLRERRL
                     RRKASELEAVVRLRTRELETARDAAQAAMESKARFLAHMSHEVRTPLSAVLGYADLLA
                     EELRERGADDLLPDVEKIERAARQQLSLVNEALDLSKLEAGGAELHLTEFDAEALVRE
                     AAETAWPLLKKGRNRLETRGVDGLGRVLSDEGKLRQVLLNLLSNAARFTEGGTVSVEA
                     AIDGGFLTIRVADTGIGMTPEQRQRIFTPFAQASPGHAARFGGTGLGLVISRGYCELL
                     HGSLELTSEAGRGSTFTVRVPARLDRRTSGR"
     gene            complement(15648..16364)
                     /locus_tag="P5164_00835"
     CDS             complement(15648..16364)
                     /locus_tag="P5164_00835"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HRY42462.1"
                     /translation="MVALLGGLLGSGCPGSTSGPDPTARARAGMVDRQIVARGVKDDR
                     VLAVLREVPRHLFVPPDQAAQAYEDHPLPIGSGQTISQPYVVAFMSEQLRLTGTEKVL
                     EIGTGSGYQTAVLSRLAARVFTIEIRPELAEQAAARLKEFGAENVELRAGDGFFGWPE
                     EAPFDAILVTAAPEKVPEPLVAQLAPGGRMVIPVGGFYQELKVLERTPDGIRETSVLP
                     VRFVPFVGEAERHREGEGER"
     gene            complement(16507..18195)
                     /locus_tag="P5164_00840"
     CDS             complement(16507..18195)
                     /locus_tag="P5164_00840"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRY42463.1"
                     /translation="MRADVPGAHALLATLDPASAGNALLEPVLEALLKGAGGSRGLAV
                     DAGARCHPAARGLDPLPSELAELSSGLAKGGAPGASRGPELLVRIFGTEPPEGEPPFD
                     PRVVPLTVGDETLAWVALERSPADPVGFASVAAHAASLLAWVRMSEKVREADFELKYR
                     VWELESIYDVGLSIARTLDLESLADEILMKSVSLLNARSGTLVVRPGGPADGGNAVFV
                     RSFGTPLVEPGSVEEPDGAVLVCNRREDRPASLAGAPAEKLLAVPISSEGRPLGLLVV
                     ADKENRQGGIDDFGPADVRILSLFGNQAAIALENARLHREAVEKEKMEREIEIAASIQ
                     RAILPTTLPAVSGILIAAGNRPTRQVGGDFYDVYPLPDGRVAFCVADVAGKGIPAALL
                     VSTVHACIHLLVEAGSSDLTALVARTNRHLARFSSTRKFVTLFIAVYDPSDRSLRYVN
                     AGHNPGIRISAAGTELLPSGGVPIGMFESATQAESRLVLAPGDLVVLYSDGITEAVDA
                     KDEEFGMERLTRLLESGRALPPNVLKDRIFAAVESFTRGVAQYDDQTVLVARPT"
     gene            complement(18205..18567)
                     /locus_tag="P5164_00845"
     CDS             complement(18205..18567)
                     /locus_tag="P5164_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRY42464.1"
                     /translation="MSETLKVTLEKPDGTVAILRTDGYINNTGGEEIARQAYTLLGEG
                     TVRLLLDLEKTKIVNSIGISILIEVLEKVLDQGGRLAFCRLTPTIEKTFQIMGLAQYA
                     RIFPAPEDALTWLRGEAE"
     gene            complement(18577..19020)
                     /locus_tag="P5164_00850"
     CDS             complement(18577..19020)
                     /locus_tag="P5164_00850"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY42465.1"
                     /translation="MPTADTREVHLTIPVAPEMEIAATAQVAALGEWMELGRDKIDEV
                     KMAVVEACINAFEHSGTPDHRVELRFRVGTERSENGGRAYLEVDVLDNGHGFDPACVA
                     SPEIGKKLRAPHKRGWGLRIIRSLMDDVRIESGERGTMIVMRKYR"
     gene            complement(19038..19262)
                     /locus_tag="P5164_00855"
     CDS             complement(19038..19262)
                     /locus_tag="P5164_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42466.1"
                     /translation="MERSMGLSDRPVPGCPVGRARAEGDRTAPVEGERRVPGLLSLLL
                     LGEAAAFAALQVSGNIPEIVTVLVRALLLL"
     gene            complement(19487..20974)
                     /locus_tag="P5164_00860"
     CDS             complement(19487..20974)
                     /locus_tag="P5164_00860"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRY42467.1"
                     /translation="MAVRVARSSESFDLRKKVLRLETLYDVSRALNVLRDEQALVDEI
                     VARAVALLDAERGFGVVFEEQSGPAVVSTVSFPTFPGSLAAASDPFVLDLCRARGPLS
                     RTGETVLGSAAGSVVGAPLVVRDRVLGVLVALEREARGGGAAAFDDGDRRFLESVAAL
                     AAPALDALRQLRALEADVDRLREENRALKGSLGIDELLVGDSAGMRRVKELVVRAAAS
                     RVGVLIRGESGTGKELVARLLHAASPRRDGPFLALNCAAVPETLLEAELFGIERGVAT
                     GVEARQGKFELATGGTIFLDEIADAPLAIQAKLLRVLQEREIERLGGRRRIPVDVRVV
                     AATNADLERSIREKRFREDLYYRIRVVEIPLPPLRERREDIPRLIGHFLSRIAAREGR
                     RPKSLAREAVARLLDYPFPGNVRELENLLEGAVALVPGETIEAGDLQLRGGRTGPGSD
                     GSLDLATLELEHIRRVLESVGGNKSEAARILGINRRTLYRKHVSN"
     gene            complement(20976..22394)
                     /locus_tag="P5164_00865"
     CDS             complement(20976..22394)
                     /locus_tag="P5164_00865"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY42468.1"
                     /translation="MTKPRVRKLFLAAAALAVVAGGTAFAVLARDRREVTTRSAEAYR
                     FYRQGLENERRLYHKDALAAYAKALELDPHFAMATLRLAGLFAGRDRDRARSLVQCAA
                     QSREVLNEHERLMLDIAEAELLAGDPKRAAERIRAYVERFPEDPEGYFRLAGVLVREG
                     KPDETLATYERLVALDPNQAIAYNYLGYYFVRRRDFVKGEDFFKRYRFLAPDQANPYD
                     SLGELYANTGRYAEAEEHLKRALEIKPDFLPAVGHLGTLEASRGNAEGAVTWYRRASD
                     MAETWKERYAFAGAAVMALLDVGRPEDALALLDEVVAAAPHRDGKDAVVIRWSYLRSR
                     AIVLALAGRVDEAEAELAGLPELPPDLDADGKMALEKSLSGPKAAFASARGDLEGAVR
                     HFREILAAETERGMGDFGYYPSIFRVRARLADALVALGRREEAREELRPVLAVNPRFA
                     PALEVLARIDGRAAATTGDAGR"
     gene            complement(22432..>23921)
                     /locus_tag="P5164_00870"
     CDS             complement(22432..>23921)
                     /locus_tag="P5164_00870"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HRY42469.1"
                     /translation="RLFDVQVIVGKSIVYAILTAIVTGLYALAVVAGNALVSSRTLSP
                     LFAFAFGLAVVLLVDPLRRRMQAVVDRVFFRDRADFQSAFRDISRQVVAQLDRGKMQQ
                     LLTERTAELLRLSRLDLLTPRPEDGALADPRRGDVALPVGAALGRLLVERDAPVPLRD
                     LDAWALDAPSRTFLKAQLERGVRVLVPVATRGRLLGVLAAGEKKSEEEFHKEDLDNLV
                     TIANQGALGLEVAALHEQLTRRAEVERDLEIARDIQVSLFPRELPRIAGIELYGVSLP
                     AKVVGGDFYDFLPVDGGFDGRVALVVGDVSGKSIPASLLMVAAKEIVYARSMQDPEPS
                     TVFRESNRRIYEIKRRMFVSLGYFLYDPLQLTLHYSIGGQPLPLVVRGGEAVELPAPV
                     SRLPLGALRDTTYDAKTFWLRKGDLLLFYTDGLNEAMSVENAYFGDERLKASLVRHSA
                     APLPEMAEEILEDIRQFTFGAEQSDDATFVLLRVTGAKPAPAGSV"
BASE COUNT         3332 a         8744 c         8793 g         3052 t
ORIGIN      
        1 ctcgggcact tcctcggcat ccaggtgcac gacgtcgccg ggcgccaggc ggaccgcgtg
       61 gggacgctcg cccctccgcc cgccgagtac cccgcgctcc ggacgacgcg gacgatcgag
      121 gcggggatgc tcttcacgat cgagccgggg ctctacttca tcccgatgct cctcgagacg
      181 caccgggagg gggcgaaccg ggccgacgtc gactgggcga tcgtcgacag gctcacgccg
      241 ttcggcggca ttcggatcga ggacgacgtc tgggtcggcg aggacggagt ccgtaacctg
      301 acccgccccg agctgccatg aacgcggggg tttctcgatc cccccgcgcg gtcctgttcc
      361 cgggggtttc tcgatccccc cggccccccc gcgaggagaa ccacgagaga cggtgccccg
      421 aatgaggctc tccggcgaat cgccccgcga ggcgggattc gcgcagcccg cggaatgggc
      481 ggctcacgag gcgtgctggc tcgcctggcc gagccacgag gagctctgga aggaggcgct
      541 cggcccggtg cagggcgcct tcgtccgatt cgcccgcgcg atcgccgacc cgggccccga
      601 gggcccgcgc ggcgagcggc tcgaggtcct cgcgccgacg gccgggcgcg ccgccgaggc
      661 gcgggcgcgg ctcgaagggc tcggagcgcg cgtccacgag atcccgttcg gcgacatctg
      721 gatgcgcgac atcgcgccgg tcttcgtgac gtcggaggcg ggcgaggtcg cggccgcctc
      781 cttcgtcttc aacggctggg gaaacaagta cgacctcccc ggcgaccggg aggtctcgaa
      841 gcgcgtcgcc gaggcgtcgg gcctccgggc tttccgcgac gagctcgtcc tggaaggggg
      901 ctccgtcgag ccggacggcg aggggacgct cctgacgacg cggcagtgcc tcctgaaccc
      961 gaacaggaac ccgggcctct cgcaggcggc gctcgagcgg cggctccggg acggcctcgg
     1021 cgcggagagc gtcctctggc tcggcgacgg cctgatcaac gaccacaccg acgggcacgt
     1081 cgacacggtc gcccgcttcg tggccccggg cgtcgccgtc tgcatggagc cctccggcgg
     1141 cggcgacccg aacgccgtcg cgctgaagga gatcctccgc gacctcgcgt cgatgcgcga
     1201 cgcgaagggg cggcggctcg aggtcttcac ggtgccgtcc cccggcgtcg tcaccgaccc
     1261 cgacggcgag ctgatggcgg cgagctacgt caacttctac gtcgcgaaca cgacggtcac
     1321 cgtcccggtc tacgggacgc cgaacgacgc gcccgccctg aagcggatcg agtcgctctt
     1381 ccccggccgg cgcgtccatg acgtccccgc gcgcgagctc ctcctcggcg gcggggcgtt
     1441 ccactgcatc agccagcagc agccgaaggg gggccgcgga tgacgtccga gacgctctcg
     1501 atcgggatcg tccagtgcgc gctcggcggc tctcgcgagg agaacgcgga ccgcgtcctc
     1561 ggcctcgttc gcgaagcggc gaggctcggt gcaaagctcg tcgtgacgcc ggagctcctc
     1621 gaggggccct atttctgccg cgaggagaag gacgcgttct tcgagtgggc gcgccccgcc
     1681 gaggggaacg ccacgctcgc gcgcttccag acgctcgcga aggagctcgg cgtcgtcctc
     1741 cccttctcgt tcttcgagaa ggccgggaac gcctactaca acaccctcgc gatggtcgac
     1801 gccgacggaa cgctcctcgg cacgtaccga aagagccaca tccccgacgg cccgggctac
     1861 gaggagaagt tctacttccg ccccggcgac accggcttca aggtcttcga cacgaaggcc
     1921 gggaaggtcg gcggggcggt ctgctgggac cagtggtacc ccgaatgcgc gcgggcgctc
     1981 gcgctcctcg gcgccgacgt cctcgtctac ccgacggcga tcggctccga gcccgcgaac
     2041 cccgcgctcg acacgcgcga ccgctggcgg cgcgcgatgg tcggccacgc ggtctcgaac
     2101 gtcatccccg tcgccgcgtc gaaccggatc ggcgaggagg acggccagct cttctacggc
     2161 tcctcgttcg tggccgggac ggacggctcc ttcctcgccg agatggggcg cgacgagacc
     2221 ggggtccgcg tcgtcacggt cgacctcgcg gcgatccgcc gcgagcgcgc ctcctggggc
     2281 ttcttccgcg accggcggcc ggagctctac ggcgtcctga cgacggccga cggccggcag
     2341 aaggtctgat cgggagcggg gaggcctcgc ggcctccccg ccgcgcctca agccaggtcg
     2401 aagacgagga gctcggcctc cgctcccgcg ccgcgcaggg tgaaggaggt ctcgtccgag
     2461 gcggcgaggc cgtcccccgc ttcgagccgg tggccgttca catcggcctc gccccgcgcg
     2521 acctggaccc agaggtggcg cccttccgcg atctccagcg ggacgctctc cccttcgcgg
     2581 aggatcgtgg agaagaggcg cacgtcctgg cggatcgcga tcgagccggc ctcgcccgtc
     2641 tcggacgcga ggagccggag ccgcccgcgc ttctcctcct cctcgaaccg cttctgcccg
     2701 tagcgcggtg cgtagccgcg ccggtcgggg acgatccaga tctggaggaa atggaccggc
     2761 tcgctcccgg aggcgttgaa ctcgctgtgg cggacgcccg tcccggcgct catgtactgg
     2821 acgtcgcccg ggacgatcgc ggagccgttc cccaggctgt cctcgtgggc gagagagccc
     2881 gagaggacgt acgagaggat ctccatgtcg ttgtgcccgt gcgtcccgaa gccggcgccg
     2941 gggacgacgc ggtcctcgtt gatcacccgc agggagcgga agtggacgtg ctcgggatcc
     3001 cagtagtcgg cgaaggagaa ggtgtggcgc gtgtcgagcc agccgtggtc gaagcgaccg
     3061 cgctcgtcgc tctttcggat cgtgatcatc gaggacctcc ccgggacccc gcggccggga
     3121 tccctcttcc gggttgttgc agggaccggg ccacccccgc acgaacctga tggacggccc
     3181 ggagccgcgc cgggccgcag ggaaccggcg ccgagcgcgt ctcggcgggc cgggacccgt
     3241 cgattcgaga ggcggcgacc tctcgctttg agaagatccc cccatgcgcg cagacgacct
     3301 gacgctcgac gccctcttcg acgccgatcc gaagggcggg atctaccgct tcgccgggga
     3361 gcgggcgatc ctcctcgaca cggccgcgct cgggctcctc cgcaaggagc tcatcgaaac
     3421 gctctctctc agcgtggcgc gcggggtcct ctaccgcttc ggctgggcgc acgggtggcg
     3481 gacggcagag gcgatgcggg acgcgttcga gtgggacggc cccacggagt ggattcacgc
     3541 cgggggacgc ctccaccggc tgaaggggct cgtcacgttc cgtcccgtcc cgcgggcgcc
     3601 gaacgagccg ccgaggcact tctcccaggc gttctgggac gagtcgtacg aggccgagca
     3661 gcacctcctc cacttcggac gctccgacga gccggtctgc tggaccctca ccgggttcgc
     3721 gagcggctac ctctccgcct gccacggccg gtcgatccgc gtcgtcgagg aggagtgcgt
     3781 cgggaaaggg gacccggcct gccggatggc gggagagccc gaggaggact gggacgaggc
     3841 gacccggaag gcgctctcgg gcctctcgga gcgcggactc gagggagagc tggggcgcgt
     3901 ccgcgacgct ctccgcgcga ccgagcagaa gctgaaggcg agaaggaacg agctgacgcg
     3961 cctgaaggtc gaggagccgc acccgggcgt cgtggcccag agccccggga tgcggcgggc
     4021 gttcgacctg gcgtcgcgcg tcgcgcgcgt cgactcgacg atcctcctga cgggcgagag
     4081 cggctcgggg aaggaggtcc tcgcgcgggt gatccacgag cgctcgcccc gcgccgccgg
     4141 ccccttcgtc gcgatcaacg gcgcggcgct tcccgagagc ctcctcgaga gcgagctgtt
     4201 cgggcacgcg cggggcgcgt tctccggcgc cgcctccgac cgcgccgggc tcttcgaggc
     4261 ggcgaacggc gggacgctct tcctcgacga gatcggggag gtgccgctgg gcctgcaggc
     4321 caagctcctc cgcgcgctcc aggagcgcga ggtgcggcgg gtcggcgaga accggagccg
     4381 gaaggtcgac gtccgcgtcg tcgccgcgac gaaccgggac ctcggaaagg acgtcgagac
     4441 ggggcacttc cggcgcgacc tctactaccg gctccgggtg atcgagatcc cgatcccgcc
     4501 gctccgggag cggccggagg acgtcctgcc gctcgcgagg gccctccttc tcgacgcggc
     4561 gcgccggacc ggtgcgcagg tggccggctt cacgccggag gcggcgcgcc tcctccagcg
     4621 ccaccgctgg cccggaaacg tccgcgagct ggcgaacgcg gtggagcggg cggcggtcct
     4681 cgccgacggc gcgcgcgtcg gcgcggagga cctcccggac gagatcgtcc gtcccgcgcg
     4741 cggcgggggc gccgagggcg agacgctcgc cgaggtggag aaggcccacg tcctcttcgt
     4801 cctcggcgag gaggacggcc accgggagaa ggccgcgcgg cggctcggca tcggggtcgc
     4861 gacgctctac cggaagctga aggagtgggg cgacccggga cggtaggcgg tcagacctcc
     4921 gcgtcggcgg cccggccgcc gaagagggag agctcgtctc ccgccgcgtc gtagtcgagg
     4981 agctgggcga agcgccccca gttcacgagg gtctcgaaga gcgcccccgt cgcctccgcc
     5041 gggaggcgat ccgcaagcat ctcgcgcacc tcctcgccgg gggcggccgc gtcctcgcgc
     5101 cccgcgaggc cccggacgac cgtcgcgaag gtgccgagcg cgaggagctg gcgccggaag
     5161 aggaacggct tctcgcgcgg cgccgccgcc acgacgccgc ggccggcgtc cgtgaggagg
     5221 acacggttct tcggcgtgtc gacgaggtcg aggagctcgg ccgccttgac gacggcgatc
     5281 gtccggccga agtcccactc cgtcatcgcg tcgacctcga agaggtccat ctcgccgccg
     5341 tggtccgaga ggatctcgag gaggcccagc atctcgccga cggtcgccgg cggcagcggg
     5401 acgaaggggc ccggcccggg gcgccccggc gcggccggcg cgggcgcctc ctcctcttcg
     5461 gggaggtgaa cgcgcgcgac ggcgtcgtgg atccggtcga ccatccggaa gaactcgggg
     5521 tggcgcgggt cgcgcgggtg cgggagcgtg ttccggatct cgcggcggac ggtgcccggg
     5581 ttcgcgccca ggacgacgat ccggtcggcg agcgtgaccg cctcctcgat gaggtgggtg
     5641 acgagaagga ccgaggagag gccggtgccg ccgtcggacc agaggcgata gacctcggcg
     5701 cgaaggagct cggccgtcag gacgtcgagc gccgagaacg gctcgtccat gcagaggagc
     5761 tccggtcccg agacgagggc gcgcgcgatc ccgacgcgct gcttcattcc gccggagagc
     5821 tcgcgcggca tcgcccgctc gtgcccggag aggccgacgc gggcgacgac gtcccggacg
     5881 cgggcggcgc tttcggggtc ggggacgccg cgccccgtca ggccgatccg cacgttctcc
     5941 tcgacggaga gccacgggaa gagggcgaag ctctggaaga cgagggccgc tcccgggtgg
     6001 atgccgcgga tcggcgcgcc gtgggcgagg acctcgccgg cggtcggctc gatgaggccg
     6061 atgaggatcc ggaggagcgt cgacttgccg caccccgagg ggccgaggac ggcgacgacc
     6121 tccccccttc ggacctcgaa gtcgacgcga tcgaggatca ccctctcgcg ccccgcctcg
     6181 tcgcgatagg tcttgcgcac gccgcggacg gcggcgatcg ggagctcgtc ggcgctcaac
     6241 ggttcagagc gtagcgcgtc gcggcgacgc cgtagagcct ccgccagacc gtccggttca
     6301 ggaggacgag ggcgagggac atcgtgagga cgcccgcggc gagaaggggg aagttcccgt
     6361 cgtgcgtggc gcgggtgatc agcgagccga gcccgaacgt cgagagctcc gtcccccgga
     6421 aggccagcgt ctcggcgacg atcgaggcgt tccaggcccc gcccgcggcg gtgacgaggc
     6481 ccgtgacgag gaacgggaag acggacggga ggtcgagcgt ggcgaaccgg cggagtcccg
     6541 tgacgccgta gacggcggag gcctcctcga ggtcccccgg gatcgcgctc gcccccgcga
     6601 ggacgttgaa gaggacgtac cactgcgcgc cgagccccat gaggacggcg gcgacgacgg
     6661 cgaacgggac tcggagcgcg aggaggccgg ccgtgacgag cgggtagagc atcggcgcgg
     6721 gaaacgaggc gacgatctgg acgaccggct ggaggcgccg cgaccattcc ctcgagcggc
     6781 cgaggaggac gccgagcggc accatcacgg cggtcgcaac ggcgagggcg ccggccgccc
     6841 ggagggcggt cgcgccgagc gcgagcgcga ggaggcccca ttcccgcacc ggcacgcgca
     6901 ccaggaggcc gccgaggcgc accgtccccc agagcgcggc cgcgacggcg agcccgcccc
     6961 cgaggatcgc ggcggcgcgc agggcgagag cgcggcgggg cgcgccgcgc gcccgggccg
     7021 ggccgctccc tgccgcccgc gcgcgcgccg ccgcgcgcgc cttccggagc gagcgcgtcg
     7081 cccgacggag aagcctcgag cggccgacga gcgagacgac ccacgacgcc ggcggctcgc
     7141 ccgagcccga ctcctcgacg cggaaacgct gcgaccagac gacgagcggg cgccagagga
     7201 gctggtcgac cgcgacgatc atcgccgtca tcgcgacgac ggcccaggcc atcgccctcg
     7261 tgtcgccctt ctcgatcgcc accgccatgt acgagccgac gccggggagg cggaagtccc
     7321 gcccgccgag gacgaacgcc tcgttgaccg tcaggaagaa ccagcccccc gccatcgaca
     7381 tcatcgagtt ccagacgagg ccgatgacgg ccgaagggag ctcgacgacg cggaaggtct
     7441 tccagcggga gaagccgttg aggcgcgcca cctcgtacat ctcggccgga acgcccttca
     7501 gcgacccgta gaaggagaag gccatgttcc agacctgccc cgtgaagatc atgacgacgc
     7561 acgcgagctc gaggccgacg ttcgtccgcg ggaagagcgc gaccatgccg aggaccagcc
     7621 ccgggaggaa cccgaggacc gggatcccct gtccgatgtc gaggagcggg atgaggaccc
     7681 gctcggcgcg ccgcgatcgg gccgccgcgg tcccgacgac gagcgtgaag aggagcgaga
     7741 ggacgtaggc ggcgaggccg cgcgagagcg agaggagcgt gtagccgggg agcgccgagg
     7801 gtacgaggtc gatcggcgcc gcctcgcgga gcggggcccc ccactcctcg gcgagcgcga
     7861 cgatcccggc gatcgaggcg ccgagggccg cgaggaggag caggtcgatc gcgcgccccg
     7921 ccgcggcgga gccgggcgtc cggggactcg gagcgacccc gacgggtgcg gccgtcacgg
     7981 cgcgaaccgt agcagagcgc gcggccgggc gcggcgcgat tcgcgattcc gccgccggcc
     8041 gcgggccgcc gtggcccggc cccccgattg catggagggt ccgcatgttc gagcgacacg
     8101 ccgagcccct cctctccggc tccggcttcc tccgccgcca gctgctccat gcgggagctt
     8161 cggggctcct cgtcggagcc gccctcgcca tcggggtcct cggctaccac ctcctcgaag
     8221 ggctcccgtg ggtcgacgcg ctcctcgacg cgtcgatgat cctcggcggt atgggtccgg
     8281 tcgacccgat ccggaccacg ggcgggaagc tcttcgcctc ggcctacgcc ctcttcagcg
     8341 gcctcgtctt cctcgcggcg gcgggcgtcg tcttcgcgcc cgccttccac cgcttcctcc
     8401 accgcttcca cctcgaggcg gacgacgacc ccggcgacga gactcgggga gagcggtgag
     8461 ccccggccgg cgcgggcggc gcgccgtcgc gacgctcgag ggcctgaagg gagcgctcgt
     8521 cctcctcgcg ggcctcggcg cgttcgccct cgttcaccgc gacgcgcagg cgttcgccga
     8581 gcacgtcgtg cgtcacttcc acctgaaccc ggcggggcac gccccgcgca gctttctgga
     8641 ggcggccgat cacctcgacg acgccaggct ccggtggctc gcggcgggcg ccttctccta
     8701 cgcgcttctc cgcctcctcg agggctgggg cctcttccgg gagcgggagt gggcggagtg
     8761 gctcggcgcc ctttccgccg ccctctacgt ccccttcgag gtcgccgcgt tcgtctcccg
     8821 accttccccg ctgaggggag cgctcctcct cctgaacctc gccgtcgtca cgtacctcgc
     8881 cggcctcctg ctcgagcggc gacgccggcg gccgtgacgc gggtcgaagg tccgcaccga
     8941 gctcctccgg ctcccttacg acggcccctt cgacctcctc tccttcgagg aagcgagcgg
     9001 ccgcgtcacg ctcggaggca gggtcaacct cccgatgctc cggaagcagg ccgaggaggc
     9061 cgtccgcgag cttccgggcg tggaggaggt cgaggaccgc ctcgaggtcg atggaccggc
     9121 ccggagctga cgcgcccgcc gacgcgggac gatccggcgc gtacgctccc gcgccggggc
     9181 gccccgccgg cgtcgccgag ctcgagcggc tcctcgccga cccgcgcgtg cgggagaaga
     9241 tcgccgcgat cgcgagggag gaggcggctc cggaggagga gtccgcgccg acacgctggc
     9301 agcgcttcag cgccttcgtc gcgggcgcga gcagcgcgct cgtcgtcgtc ctcgcgttcc
     9361 tcgtcccgtc catcgaggag cagtgggatc gcttccaggc gcgccgcgtc gtgcagaagc
     9421 acgtcgacct cgggagggcc ttcgtcgcgg aagggaagta caagctcgcc gaggagtcgt
     9481 tcgggcgagc cttcgagctc tccgagaaca agcgcctcga cgtcgacgag gagcggctcg
     9541 aggcgaaggt gagggccgtc gactccgacc ccgactgggg cgccgcgaac ccggaggggc
     9601 tcgaggagtc ggacttcctc tacctcctga agctccagga gggcgccgga cacgtccccg
     9661 agcgggcggc gacgctgaac gggtacggga cattcctcgc ctccgcgaag cgctggcggg
     9721 aagcggagga gcggctccgc gaggcggcgc ggctcgaccc gcgctccccg gcgccgctcg
     9781 tcggcctcgg gaacctcctc cgggaccggg ggcgcgcgcc ggaggccgag tcgttctacc
     9841 ggcgggcgct ccgcctcgac ggccggaacg gatggatcca ctacgacctc gcgctcctcc
     9901 tcggcgagac ggggcggccc gcggacgcca cggcggagct cgagcgcgcg gctgcgtgcg
     9961 acccgtccga gccggagctc ctccgcgcgc tcgcggagca gctcgagcgg acgagccgga
    10021 aggaggaggc tcgcgccgtc cgcggccggc ttctcgcgct cgacccgccg ggcggggagg
    10081 aggggcgccg cgccggggcg cgcggctgac gacgcggctc gggcttccgg cgccgggggc
    10141 cggctccccc ggccggcctc acacccccac cgagccttcg tcgtccgcag cggcctcctc
    10201 gtcgccttct cccgagccct cctcctccgc gccgtcctct gcgtcggccg gacccgggca
    10261 cgccccgccc tccacccggg ccgctccgtc gtcgctcacc gacgcgagcc gggcgaacca
    10321 gcctcctgcc gccgcgagct cctccggcgt cccttcctcg acgacgcgcc cgccgtcgag
    10381 gacggcgacc cggtcggcgt cgcggagcgt cgcgtagcgg tgcgcgatga ggagcgtggt
    10441 cggctgcggc tcgatccggg cgagggagcg cttgatctcc gcctccgtct cgacgtcgag
    10501 gttcgccgtc gcctcgtcga ggacgaggat ccgcggacgc gagacgagga cgcgcgcgag
    10561 ctggagcctc tggcgctcgc cgacggagag gccgacaccc cgctccccga tcggcgtctg
    10621 aagcccctcc gggagcctct cgagagccgg tccgagcccc gccgcgagcg ccgccgagcg
    10681 cacctcctcg tcggtcgcgt cggggcgctt gtagcggatg ttgtccgcga gggagccccg
    10741 gaagacggcc ccgtccgcgg cgacgacgcc gatctcccgg cgcacggtcg aagggtcgag
    10801 ccgcgagagg ggctggccgt cgacgaggac ctcgccggac tgcggctcat agagcttcag
    10861 gaggaggtcc gcggtcgtcg tcttccccgc gccggacgcc ccggcgagcg ccgtgaccgt
    10921 ccccggcgcg agcgtgaggc tcaggccccg gaggacctcg cggccctccg tgtagccgaa
    10981 gcggacgtcg cggaactcga ccgcgccggg tcccgccgcg agggcgctcc cgccctcctc
    11041 tccccccgcg gtctccagga gccggacggc gcggcggagc gaggcgacgt gctgctggag
    11101 cgtgaccgcc atgctgctca aggagtcgat cggcccgtac agccggtcca gataggcgac
    11161 gaacatcacg acgtcgcccg gcgtgagctg gcgctcgagg acgagccatc cgccgtaccc
    11221 gaagacgagc gccttgctga agtgggagac gacgacctcg ccgaggagat agcggttcga
    11281 gagccggttg cgggcgaggt agtcgtcgta cgcccgcgtc gcggacccgc ggagcttctc
    11341 ggcctcgcgc ggctcggccc cggagagctt caccgtcttg acggacgcga gcgcgtcctg
    11401 gatccgggcg gagacctcct cccacttctc gtagtacccg tcgagcccgg actcgagccg
    11461 gcgcgcggag cggaccgcga ggaggaggta gagcgggagg agcgcgagcg cgacgatcga
    11521 gagccggccg ctctgccaga ccatgatcgc gaagacgccc gccatccgga tcgcctcggg
    11581 cgcgacctcc tgcgcgaagg cggtgacgat cggtgcgacc tggtcggact ggtcgatctg
    11641 cttggcgagg ccgccgctcg cgcggcggct gaagaacgcg agcggcaggc ggaggacgtg
    11701 cccgaaggtc gactggatca ggtccgcctc gatccggctc gcgaggacgg tgctgcggtt
    11761 gtccgccgcg agcgagaaga cgtagccgag gacccccgtc acgaggagga gcccgaccgc
    11821 gacgaggagc gtcgagatcg cctgctcggg cgtccgcggg gcgacgtagt cggagcggtg
    11881 cggctcgcgg gcgggcggcg gcgccggggc ggccgtcgcc ttcggccccg gcgcaccggg
    11941 cctcgcggcc cggcggtggc ggcggtgggc ggacggccgt cctgccgtcg ccgcgccggc
    12001 gttccccgcg gtcgggacgg cggtcggcac gggcgtcggg acgaggagcg cgggggcgcc
    12061 tccctccgcg gctggagcgg cccgggggcc gttggccggc ggcgccgcct cctcctcgtc
    12121 ctcgagcccg gacgaggcgc cggcccccgc ccgagacacg aagagccccg cgacgtcgtt
    12181 caccgcggcg cggtagacga gcggctcgac gaggtccgcc gccgtaccga ggagcgcgag
    12241 gaccgcgatg acgaggaacc ggccccggta gggccgcgcg agcccgagga ggtggcgcca
    12301 gacgccgtcg tcgcttcgca tcccgatcgg ggaacgatga gcgcgatcgg gcggcgtcgc
    12361 aagcccgaac gggaggcgcc ggaggtgccc ggcggtcctc ccgccggcgc cggctcagcg
    12421 ccccgaggtc cgcctgtcga gccgcgccgg tacgcgcacg gtgaaggtcg agccccttcc
    12481 cgcctcgctc gtgagctcga gcgagccgtg caggagctcg cagtagccgc gcgagatgac
    12541 gaggccgagg ccggtcccgc cgaaacgcgc cgcgtgcccc gggctcgcct gcgcgaaggg
    12601 ggtgaagatg cgctggcgct gctcgggcgt catcccgatc ccggtgtcgg ccacgcggat
    12661 ggtcaggaag cccccgtcga tcgcggcctc caccgaaacc gtccccccct ccgtgaagcg
    12721 tgcggcgttc gagaggaggt tgaggagcac ctgccggagc ttcccctcgt cggagaggac
    12781 ccgcccgagg ccgtcgacgc cgcgggtctc gaggcggttg cgccccttct tcaggagggg
    12841 ccacgcggtc tcggccgcct cgcggacgag ggcttccgcg tcgaactcgg tcaggtggag
    12901 ctcggcgccg ccggcttcga gcttcgagag gtcgagcgcc tcgttgacga gggagagctg
    12961 ctgccgggcg gcccgctcga tcttctcgac gtcggggagg aggtcgtcgg cgccgcgctc
    13021 ccgcagctcc tccgcgagga ggtccgcgta gcccaggacc gcgctcagcg gcgtccgcac
    13081 ctcgtggctc atgtgggcga ggaagcgggc cttgctctcc atcgctgcct gcgccgcgtc
    13141 ccgcgccgtc tccagctcgc gggtccggag gcggacgacc gcctccagct cgctcgcctt
    13201 ccgtcgcagc cggcgctccc gcaggtggac gaccgccagg ccgagcgccg ccgcgaggag
    13261 cgaccagagg acgagggcgg cggggcggcg ccagaagggc tgggtcaccc ggaacgaaac
    13321 ctccagcggg cccgagaccg tcgcgtcggc gtcgcgcccc caggcccgga agacgtactc
    13381 cccgggaggg aggttcgtgt actcgcggcg gtgcgccgtc gtccactccg tcggcgcggc
    13441 ttcccgcccg acgagctgcg tccggtagcg gacgagcgac tggccgtgat aggcgaggag
    13501 cgcgtactcg aaggcgacgt cggcctctcg ccgccggagg gtcacctccc cctcgccggg
    13561 gcgcgcggcg ccgccggcga cgaaacgctc gaggacgagc gggcggggag ccggcttcgc
    13621 cgcccgggcc cgggccggat cgaagatcgc cgcgcctgcg gccgtcgaga tccagacgcg
    13681 gccggccccg tcgacgaggc tcccctggtt gcaggcggcg ctcggcaggc cttcgttcgt
    13741 gccgaacgtg gagacctcga gcccgccgcc cgaccgggcc ggctcgaggc gcgcgacgcc
    13801 cctgctcgta ccgaggaagg cggagccgcc ggggaccggc gcgatcgaga ggacgacgtc
    13861 gctcgggagc gggggccggc tctccgtggt gaggatcgac caccccgcgt cggggtcgtc
    13921 gaggctccgg cgcgcgaggc cgccgcgcgt cccgacccag agctcgcgca ggccgcccgg
    13981 caacgccacc tcggcgagcg cgaggacgac gttgttcgga agtccgctcg tccggtcgtg
    14041 gaccctcgcg acccggcctc ccgagacctc cgcgaggccg cccccgcgcg tcccgaccca
    14101 gagggtcctc ccgggagggc tccgggtctc gaggagcgag aggacggagc gggccgggag
    14161 cccgtccccc tccccgagcg tcgtccagcg gcccgccgcg aagcggccga gcccggacct
    14221 ctcgctcccg gcccagacga cggggcgtcc cgcttcgtcc cgcgtggcga ggaggacgct
    14281 cgcgtcgtcg gagcggaacg cgggccgcgc ctcgatcggt cgccatcgtg ttccctcccg
    14341 gcgaaagatc cctgccccgg tcaggctcgc ccagagctcc gacgcctctc ccgggccgcg
    14401 aacctcggcg aacgagcgga cctcgccggc gggcagcgcc gacgccggcc cgctcaccga
    14461 gagctgtccc ccgcgaagga gggcggcccc gcggctcgtg ccgatccaga cccccggccc
    14521 gccccccggg ccgcgggtct cgaggaaaga gaagacctgg tcgcccggga ggcccgagtc
    14581 ttcgtcgaac ggcgcccagg gcgtcggctc gacgcggtga agcccggaga ggcgcgttcc
    14641 gacccagagc gtctccggcg ctccggcgcc gcccgcggcc agcgcgtaga tcgagagcgg
    14701 acccgccccc agcgccacgg gctcgaacgc gcgtccccgc agccggtaga ggcccgcgtc
    14761 gcgcgtgccg acccagaccg tctccgagcc acgggggccg gtcgccgcga ggctcgtgac
    14821 gaaccggctc ccgaggccct cctcgggtcc gaatcgcgtg acgctccccg cgcggacgcg
    14881 gaagaggccg gctccgaacg tcccgaccca gagcgaacgc tcgccgccgt cgtccgtctc
    14941 gaggagcgcg ttcgcgctga acccggagag cgacacgccg aggtcgatcg gcgcgaagcg
    15001 ccccccgtcg cccagctccg cgacgccccc ttcgcacgcc gcgacgatgc gtgacgtacc
    15061 cgaggcgtcg tgccgctcca ggaggctcca gaccctcgga tcgggaagcc cggtcccgac
    15121 cggctcgaac ctcgtcccgt cgaagcgggc gacgccgccg ccgtgggtcg ccgcccagag
    15181 cgtcccgtcc gccgcctcga ggagatggtt gacccggccg aacgggagtc cggcggcacc
    15241 gtcgaagacg tcggtcacgc cgtcccgctg cctgacgacg cctccctcct cgcggccgaa
    15301 ccagaggctc ccgtcccgcg tgccgacgac ggcccgcacg tagttcgaca ccgcccggtc
    15361 gggcatgtcg agcggggacc agatccggcc gtcgaaccgc gccgcgccgt cctgcgtgcc
    15421 gacccagagg tagccgcggc tgtcgaaggc gatcgcgtga atcgtcccct gcgggagtcc
    15481 gtcgcgctcg ccgtacgtcg cgaccgaggg gcgggcgagg tcgacctccc cgcggacgct
    15541 cgaagcctgc gcgccggcga ggaggcccag cgccgcgagg gctgcgatcg gtgcgcgcat
    15601 gctcggacga gactatcgtg acgccggggg cggagggagc gtcgggctca acgctcgccc
    15661 tctccctcgc ggtgccgctc ggcctctccg acgaacggga cgaaccggac cggcaggacg
    15721 ctcgtctccc ggatcccgtc cggcgtccgc tcgaggacct tcagctcctg gtagaagccg
    15781 ccgaccggga tcaccattcg cccgcccggc gccagctggg cgacgagcgg ctcggggacc
    15841 ttctccgggg ccgccgtcac caggatcgcg tcgaacgggg cctcctccgg ccagccgaaa
    15901 aagccgtccc ccgcgcgcag ctcgacgttc tccgccccga attccttcag gcgcgccgcc
    15961 gcctgctcgg cgagctccgg ccggatctcg atcgtgaaga cgcgggcggc gaggcgcgag
    16021 aggacggcgg tctggtagcc ggagccggtc ccgatctcca ggaccttctc cgtcccggtg
    16081 agccggagct gctcgctcat gaaggccacg acgtagggct gggagatggt ctggccgctc
    16141 ccgatcggga gcggatggtc ctcgtaggcc tgcgccgcct ggtccggcgg gacgaagagg
    16201 tgcctcggga cctcccggag caccgcgagg acgcggtcgt ccttcacgcc ccgggcgacg
    16261 atctggcgat cgaccattcc cgcacgtgca cgggccgtcg ggtcggggcc gctggtcgat
    16321 ccggggcacc ccgagccgag gagcccgccg aggagggcca ccacgctccc gattctccac
    16381 cctcgggccg gcgatgcgtg cgacgtcctc acgaggggcc attctagcga gcgccgtgcc
    16441 gccccggccg tcctgaaacg gagaatggcc tcaggcgcgc cgacccgcgg gcggcgcccc
    16501 cgcagctcag gtcggccggg cgacgaggac cgtctggtcg tcgtactgcg cgacgccgcg
    16561 cgtgaagctc tcgaccgcgg cgaagatccg gtccttcagc acgttcggcg gcagcgcacg
    16621 ccccgactcg aggaggcgcg tgagccgctc catcccgaac tcctcgtcct tcgcgtcgac
    16681 cgcctccgtg atcccgtcgg agtagaggac gacgaggtcc cccggggcga ggacgagccg
    16741 gctctcggcc tgcgtcgccg actcgaacat cccgatcggg acgccgccgc tcggcaggag
    16801 ctccgtaccg gccgccgaga tccggatacc ggggttgtgc cccgcgttca cgtagcggag
    16861 gctccggtcc gacgggtcat agaccgcgat gaagagcgtc acgaacttcc gcgtcgagga
    16921 gaagcgggcg agatggcggt tcgtccgggc gacgagcgcc gtcaggtcgg acgaccccgc
    16981 ctcgacgaga aggtggatgc aggcgtggac cgtcgagacg aggagcgccg cggggatccc
    17041 cttcccggcg acgtcggcca cgcagaacgc gacgcgtccg tccgggagcg ggtagacgtc
    17101 gtagaagtcg cctccgacct gccgggtcgg ccggttcccg gcggcgatca ggatgccgga
    17161 gacggccggg agcgtcgtcg ggaggatcgc ccgctggatg gaggcggcga tctcgatctc
    17221 gcgctccatc ttctccttct cgaccgcctc gcggtgcagg cgagcgttct cgagcgcgat
    17281 cgcggcctgg ttcccgaaga gcgagaggat ccgcacgtcc gccgggccga agtcgtcgat
    17341 ccctccctgc cggttctcct tgtcggcgac gacgaggagc cccagcgggc gcccttccga
    17401 ggagatcggg accgccagga gcttctcggc cggagcgccc gcaagggacg ccggccggtc
    17461 ttcgcgccgg ttgcagacca ggacggcgcc gtcgggctcc tccaccgagc ccggctccac
    17521 gagcggggtc ccgaagctcc ggacgaagac ggcgttcccg ccatccgccg ggccgccggg
    17581 ccggacgacg agcgtcccgc tccgcgcatt caggagagag accgacttca tcaggatctc
    17641 gtcggcgagc gactcgaggt ccagcgtcct cgcgatggag aggccgacgt cgtagatgct
    17701 ctcgagctcc cagacccggt acttcagctc gaagtccgct tcgcggacct tctccgacat
    17761 ccgcacccag gcgaggaggg atgccgcgtg cgcggcgacg gaggcgaatc cgaccgggtc
    17821 ggccggggag cgctcgaggg cgacccaggc gagcgtctcg tcgcccacgg tcagcggaac
    17881 gacccgcggg tcgaacggcg gctctccctc gggcggctcc gtcccgaaga tccggacgag
    17941 gagctccggg ccgcggctcg ccccgggagc gccccccttc gcgaggcccg aggaaagctc
    18001 cgcgagctcc gacgggagag ggtcgaggcc gcgggcggcg gggtggcatc gggctcccgc
    18061 gtcgaccgcg aggccccggg agcccccggc tcccttcagg agcgcttcga ggaccggctc
    18121 gagtagggca ttcccggccg aggccgggtc gagcgtcgcg aggagcgcgt gggcccccgg
    18181 gacgtcggcg cgcatgggtc cgtgctactc cgcctcaccg cgcagccacg tcagggcgtc
    18241 ctcgggagcg gggaagatcc gcgcgtactg cgcgaggccc atgatctgga acgtcttctc
    18301 gatcgtcggc gtcaggcggc agaacgcgag gcgcccaccc tggtccagga ccttctcgag
    18361 cacctcgatc aggatcgaga tcccgatgga gttcacgatc ttcgtcttct cgaggtcgag
    18421 caggaggcgg accgttcctt cgccgaggag cgtgtaggcc tgacgggcga tctcctctcc
    18481 gccggtgttg ttgatgtacc cgtccgtccg gaggatcgcg accgtcccgt cgggcttctc
    18541 cagggtcact ttcagcgtct cgctcatcgt cgcccttcag cggtacttcc gcatcacgat
    18601 catcgtcccg cgctcgccgc tctcgatcct cacgtcgtcc atgaggctcc ggatgatacg
    18661 aagcccccac cccctcttgt gcggcgcgcg gagcttcttc ccgatctcgg gcgaagcgac
    18721 gcacgccggg tcgaagccgt ggccgttgtc gaggacgtcg acctccaggt aggcgcggcc
    18781 cccgttctcg gacctctcgg tgccgactcg gaaccgcagc tccacgcggt ggtcgggggt
    18841 cccgctgtgc tcgaacgcgt tgatgcacgc ctcgacgacg gccatcttca cttcgtcgat
    18901 cttgtcgcgg ccgagctcca tccattcgcc cagcgccgcg acctgagccg tcgcggcgat
    18961 ctccatctcc ggggcgaccg gaatggtgag gtggacctct cgtgtgtcgg ccgtcggcat
    19021 cggcggtgct cggcgcgtca gagcagcagg agagcccgga cgagcacggt cacgatctcg
    19081 ggaatgttac ccgacacctg gagcgccgcg aacgccgcgg cctcgccgag gaggagaagg
    19141 gagagcaggc ccgggacccg gcgctcgcct tcgacgggag cggtccggtc gccttccgcg
    19201 cgggcacgcc cgacgggaca tcccggcacc ggccgatccg acagtcccat gctgcgttcc
    19261 atcgactcct cccgcgacgg aatgacgcaa cacctgcgcc agaacgaccc cgtccatcaa
    19321 acacatccta aacatctcat cttcagatac ttacagcgac agacccgatt ggcctctgtt
    19381 ttgatagcga caaaacgcca catcacatca gccccgtcag cacacaaggc tataattatt
    19441 gcaaacacaa tgagttgcgc gcaacgcgcc aaatagtccc gctgtgctaa ttggacacgt
    19501 gttttcgata gagggtccgg cgattgatgc cgagaatgcg cgctgcttcg ctcttgttcc
    19561 ctccgaccga ctcgaggacc ctccggatgt gctcgagctc gagcgtcgcc aggtcgagag
    19621 agccgtccga acctggcccg gtccggcccc cgcgcagctg caggtcgccg gcctcgatcg
    19681 tctcgcccgg cacgagcgcc accgctccct cgagcaggtt ctcgagctcg cgcacgttcc
    19741 ccgggaacgg gtagtcgagg aggcgcgcga ccgcctcgcg cgcgagcgac ttcggccgcc
    19801 gcccctcgcg cgccgcgatc cgcgagagga agtgcccgat gaggcgcggg atgtcctccc
    19861 gtcgctcgcg cagcggcggc agcgggatct cgacgacccg gatccggtag tagaggtcct
    19921 cgcggaacct cttctcgcgg atcgaccgct cgaggtcggc gttcgtcgcc gccacgaccc
    19981 ggacgtcgac cgggatccgc cgccggccgc cgagccgctc gatctccctt tcctggagga
    20041 cgcggaggag cttggcctgg atcgcgagcg gggcgtcggc gatctcgtcg aggaagatcg
    20101 tcccgcccgt ggcgagctcg aacttcccct gccgcgcctc gacccccgtc gccacgcccc
    20161 gttcgatgcc gaagagctcg gcctcgagga gagtctccgg caccgccgcg cagttgagtg
    20221 ccaggaacgg cccgtcccgg cgcggcgagg ccgcgtggag gagccgggcg acgagctcct
    20281 tgcccgtgcc gctctccccg cggatcagga cgccgacgcg ggacgccgcc gcccgcacga
    20341 cgagctcctt gacccggcgc atgccggccg agtcgccgac gaggagctcg tcgatcccga
    20401 gcgagccctt cagggcgcgg ttctcctccc ggagccggtc cacgtcggcc tcgagggcgc
    20461 gcagctggcg gagggcgtcg agcgccggcg ccgccagggc cgcgaccgac tcgaggaagc
    20521 gccggtcgcc gtcgtcgaac gccgctgcgc cccctccccg cgcctccctc tcgagcgcga
    20581 cgaggacgcc gagcacgcgg tcccgcacga cgagcggcgc gccgacgacg cttcccgccg
    20641 cgcttccgag gacggtctcc cccgtccgag agagggggcc gcgcgcccgg cagagatcga
    20701 ggacgaacgg gtccgaggcg gcggcgaggc ttccggggaa ggtcgggaac gagaccgtcg
    20761 agacgacggc cgggcccgac tgctcctcga acacgacccc gaacccgcgc tcggcgtcga
    20821 gaagggccac ggcgcgagcc acgatctcgt cgacgagcgc ctgctcgtcg cgaaggacgt
    20881 tcagcgcccg cgagacgtcg tagagcgtct cgagccggag gaccttcttg cgaaggtcga
    20941 acgactcgct ggagcgggcg acccggaccg ccatctcagc gcccggcgtc tcccgtcgtc
    21001 gcggcggcgc gcccgtcgat ccgggcgagg acctcgagag ccggcgcgaa acgcgggttc
    21061 acggcgagca cgggacgcag ctcctctcgc gcctcttccc gccgcccgag ggcgacgagc
    21121 gcgtccgcga gccgagccct cacgcggaag atcgacgggt agtagccgaa gtcccccatc
    21181 ccgcgctccg tctcggccgc gaggatctcc cggaaatgcc ggacggcccc ctcgagatcc
    21241 ccgcgggcgg aagcgaacgc cgccttcggc cccgacaggc tcttttcaag ggccatcttc
    21301 ccgtccgcgt cgaggtcggg agggagctcc ggaaggccgg cgagctccgc ttcggcctcg
    21361 tcgacgcgcc cggcgagagc gaggacgatc gccctcgagc ggaggtagct ccatcggatc
    21421 acgacggcgt ccttgccgtc ccggtgcgga gccgcggcga cgacctcgtc gaggagggca
    21481 agggcgtctt cgggccgccc cacgtcgagg agcgccatga cggcggctcc ggcgaaggcg
    21541 tagcgctcct tccacgtctc cgccatgtcg gaggcgcgcc ggtaccacgt cacggcgcct
    21601 tccgcgttcc cccggctcgc ctccagggtc ccgaggtgcc cgacggccgg aaggaagtcg
    21661 ggcttgatct cgagcgcccg cttcaggtgc tcctccgcct cggcgtagcg gcccgtgttc
    21721 gcgtagagct cgccgaggga gtcgtagggg ttcgcctggt cgggggcgag gaagcgatag
    21781 cgcttgaaga agtcctcgcc cttcacgaag tcgcggcgcc tcacgaagta gtagccgagg
    21841 tagttgtaag cgatggcctg gttcgggtcg agcgccacga gccgttcgta ggtggcgagc
    21901 gtctcgtcgg gcttcccttc gcggacgagg acccccgcca gccggaagta gccctccggg
    21961 tcctcgggga accgctcgac gtacgcccgg atccgctccg ccgcccgctt cggatcgccg
    22021 gcgaggagct ccgcctcggc gatgtcgagc atgaggcgct cgtgctcgtt caggacctca
    22081 cggctctggg cggcgcactg gacgagggac ctggcccggt cgcggtccct cccggcgaag
    22141 aggccggcga gacggagcgt cgccatcgcg aaatgggggt cgagctccag ggccttcgcg
    22201 taggcggcga gggcgtcctt gtggtagagc cggcgctcgt tctcgagccc ctgacggtag
    22261 aaccggtagg cctccgccga gcgggtcgtc acctcgcgcc ggtccctcgc gaggacggcg
    22321 aacgcggtcc ccccggccac cacggcgagg gccgcggccg cgaggaagag ctttcggacg
    22381 cgcggcttcg tcacggaacg gtcccttccg ggcccagagg ccctcggaaa tctatacgga
    22441 ccccgccggt gcgggtttcg cccccgtcac ccggaggagg acgaacgtgg cgtcgtcgga
    22501 ctgctcggcc ccgaacgtga actgccggat gtcctcgagg atctcctcgg ccatctccgg
    22561 cagcggcgcc gcgctgtggc gcacgaggga ggccttcagg cgctcgtcgc cgaaataggc
    22621 gttctcgacc gacatcgcct cgttcagacc gtcggtgtag aagaggagga ggtccccctt
    22681 ccggagccag aacgtcttcg cgtcgtaggt cgtgtcccgg agggcgccga gcgggagccg
    22741 gctgaccggg gccggcagct cgacggcttc tcccccgcgg acgacgagag gcagaggctg
    22801 cccgccgatg gagtagtgga gcgtcagctg gagagggtcg tagaggaagt agccgagcga
    22861 gacgaacatc ctccgcttga tctcgtagat ccggcggttc gactcgcgga atacggtcga
    22921 gggctccggg tcctgcatcg accgggcata gacgatctcc ttggccgcga ccatcaggag
    22981 cgaggccggg atcgactttc cggagacgtc gccgacgacg agggcgacgc gcccgtcgaa
    23041 gccgccgtcg accggcagga agtcgtagaa gtcgccgccg acgaccttcg ccgggaggct
    23101 gacgccgtag agctcgatgc ccgcgatccg ggggagctcg cgcgggaaga ggctcacctg
    23161 gatgtcgcgc gcgatctcga gatcgcgttc gacctcggcg cggcgggtca gctgctcgtg
    23221 gagcgccgcc acctcgaggc cgagggcccc ctggttcgcg atcgtgacga ggttgtcgag
    23281 gtcctccttg tggaactcct cctccgactt cttctccccg gctgcgagga cgccgaggag
    23341 ccggccgcgg gtcgcgaccg ggacgaggac ccggacgccc cgctcgagct gggccttcag
    23401 gaaggtccgc gagggggcgt cgagcgccca cgcgtcgagg tcgcggaggg ggaccggggc
    23461 gtcccgctcc acgaggagcc gaccgagggc ggcgccgacc gggagcgcca cgtctccccg
    23521 ccgcgggtcg gcgagcgctc cgtcctcggg acggggcgtc agcaggtcga gccggctcag
    23581 ccggaggagc tccgccgtcc tctcggtgag gagctgctgc atcttgccgc ggtcgagctg
    23641 cgcgacgacc tgccggctga tgtcgcggaa cgccgactgg aaatcggccc ggtcgcgaaa
    23701 gaagacccgg tcgacgaccg cctgcatcct ccgccggagc gggtcgacca gcaggacgac
    23761 cgcgaggccg aaggcgaagg cgaagagcgg cgagagggtc cgcgaggaga cgagggcgtt
    23821 ccccgccacg acggcgaggg cgtagaggcc cgtgacgatc gccgtcagga tcgcgtagac
    23881 gatcgacttg ccgacgatga cctggacgtc gaagaggcgg a
//
LOCUS       DAOTEZ010000008        23870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_1403_length_23870_cov_5.941214, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000008 DAOTEZ010000000
VERSION     DAOTEZ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 23870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23870
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1403_length_23870_cov_5.941214"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /gene="ruvB"
                     /locus_tag="P5164_00875"
     CDS             <1..948
                     /gene="ruvB"
                     /locus_tag="P5164_00875"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRY42470.1"
                     /translation="FTGQPKLRETLGVFLTAARRRNEPLDHVLLFGPPGLGKTTLAHI
                     IAREMGAQLRVTAGPVLEKAGDLAAILTNLGPGDVLFIDEIHRVPPAVEEILYPALED
                     GKLDLVIGTGPAARTVELRLQPFTLVGATTRAGLLSQPLTGRFGITHRLDYYDTASLR
                     SIVLRSAEILGCPIDEDGAGEIARRSRGTPRIANRLLRRVRDFVEVAGESTISAAGAR
                     ASLDRLEVDHFGLDELDRRILGTIVERFGGGPVGLSALAHSLGEDKGTLEDLYEPYLL
                     QSGFLTRTPKGRVATALAYRHLGLPQAASSTGPLFDGTP"
     gene            945..1856
                     /locus_tag="P5164_00880"
     CDS             945..1856
                     /locus_tag="P5164_00880"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRY42471.1"
                     /translation="MRRGLLVYNPAAGGRDRTAQMAAIADRARSKGVELALLATEGPG
                     HATEIVRLRLAERPDLVAVAGGDGTVGEAAAALVGSDVPLAILPAGTANVVAREYGVG
                     ITPEEAEAVLGSTTTRTLTTWPSAGRTSLIGTGVGFDARVMANVNPLLKRLFGRPGFA
                     LTSTLEWYRYEFPPIEVTGLDADGRPFVRRATFVLSANTRKYAGDAILSPFADPDDDL
                     LDLVLFTSEDRGDLFQFYRLLPGGRAAQLGVRGVSRLPVRSFTARSLAGYELEVQVDG
                     DAAGTTPVTVGPAGGKVRILVPSPARA"
     gene            1939..4587
                     /locus_tag="P5164_00885"
     CDS             1939..4587
                     /locus_tag="P5164_00885"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRY42472.1"
                     /translation="MRVAGAVVAACALWGAALLGTGPASAGVEPVLVASGFTAPLRVA
                     APAGDFSRLFVVEQGGRVRIVKDGVLLGGSFLDLSGRISSGGERGLLGFAFHPEYHRN
                     GRFFVNYTDPTGRTVVSEMRVSADPDRADGGSERVLLRQAQPFANHNGGHLAFSPLDG
                     TLWIGLGDGGGGGDPQNNAQSDGTWLGKMLRIDVDAKDPGKEYAVPSDNPFAGRTTPL
                     PEIWAKGLRNPWRYSFDRATGDLFVGDVGQGTWEEVDFQPASSRGGENYGWRRMEGSR
                     CYDPATGCQTGSLVLPILEYEHAGSPRRCSVTGGVVARGPSVPEIAGRYLFADYCSGE
                     LLSVKVLGGAATDPRNHRSEWMPSGGRSLATVTDIGEDARGDVYVTDASGGEVFRLTT
                     TAPTASRLVPIVLDASGRNGSRYGTELVVANRGARTATIRAKYTPAARFGGAGGGSVA
                     EILGRGEQRTYPDALAWLRTKGLPIPDASAGSQGGTLEVELTGLASASDGVVLARTTT
                     PSGTGRAGLAYPAPRAQDLATEEAVLYGLRENASDRTNLALLSGATTGTTTLRVTLVS
                     GARSFVLPEAVALEPGEWLQLDSVLARAGFSSGWAVVERVSGPGPFWVYAVFNDAGTN
                     DGSYVAPSRPVRPAQTQVVPAVVETPAFESELVLANPGPSPVTARLLWVETLATGKPR
                     RFEAAEPLAAGEQKILASFVDRLRARVPSIGARGGRYSGALFVTFETPAGRKAPGYAG
                     ARTAAPADGGGAYGLFTPGSGLAECAFREAWLFGLRQDDSARTNLALVNVEPDGGPIT
                     LRWEAFDANAGRRIGTSRDLVLGPGEWRQVDGVLGDFLLRAGYLKVSRVSGNGRFLAY
                     AVLNDGPAPGGPGTHDGSYVPMEAAE"
     gene            complement(4594..4980)
                     /locus_tag="P5164_00890"
     CDS             complement(4594..4980)
                     /locus_tag="P5164_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42473.1"
                     /translation="MLKWVQCVRRRPDLTVVEFRRQWQRYGEEIVSLGAELGAVRVEV
                     SAALAVGANAEWQARRGSAPPFDGLAEIHFSGSAPEFFAALEAPGPKERVERLEALQE
                     ELVDVSRSCFFLASQDAEASFSVGEG"
     gene            complement(5065..6834)
                     /locus_tag="P5164_00895"
     CDS             complement(5065..6834)
                     /locus_tag="P5164_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HRY42474.1"
                     /translation="MSLTLLDWAIVLAYFALSIGVGLAFTKKGGESLEEYFVAGRSAP
                     WWLAGAAMIATTFAADTPLVVTGLVAKHGVAGNWLWWNMVLSGTLTVFLYARLWRRAN
                     VMTDVEFAEVRYSGRPATFLRGFRAIYLALPINLIIMGWVTLAMVKILGIALGISPVW
                     AVGICFVITVAYSAAAGMWAVLWTDLIQLVIKMTAVIVLAVYALRAVGGMGALKEGLA
                     GRFGSLDAALSVVPPAGSRWMPAIALFTFLAVQWWAAWYPGAEPGGGGYVAQRIFSAR
                     SERDGVLATLFFNVGHYALRPWPWIVTGLCTVLLYPGGVEIAGKRDVEAAYVQAMVDL
                     LPSGWKGFLLAGFAAAYMSTMATHLNWGASYLVNDVYRRFLRPGASESHYVHAGRLTT
                     VLLFLASIVVTLNLGTIEGAWRFLLALGSGTGLVLILRWYWWRINAWSEISAMGSSGV
                     VSLAAMAWFARPGAIAPGPGADFEAQAKTMLVTVVASTIVWVTVTYLTKPEPDEKLRS
                     FYGRVRPGGPGWARVSASMGFGREPIPGGALSLANWALSAVLVYAALFGIGKLVLGAT
                     AAGLGLLALAVACFALVMRNLRS"
     gene            6921..7400
                     /locus_tag="P5164_00900"
     CDS             6921..7400
                     /locus_tag="P5164_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRY42475.1"
                     /translation="MSGRESAVETRLSSILARGRVETGFSALSPEDAVERLLRPVLLA
                     EGFSADAADGALEGIRNREGAGSTSFGAVALPHARVPRLGRIVGALGVNGEGVYASGP
                     QGIRVVLAFASPASATVEHLQFLAQVARLLRQEETVADLLEATDGAALVSAIRRRER"
     gene            complement(7397..8596)
                     /locus_tag="P5164_00905"
     CDS             complement(7397..8596)
                     /locus_tag="P5164_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42476.1"
                     /translation="MTRRRFLAHLLAGLGLRLALALFWNGSYDVYTLVRFAGIFQHDG
                     LWALYADGGRVYHFHPVVVVLYAVVLYLFSGLAGLPLQLAVKLPAIAGDLLSAGALRA
                     ARPSDGRPFLLFWWNPVSLLTTCYHGNFDSLHTALVLCAALLLARETSGRSAGLAWAG
                     AIVLKKVPALWAPALLASARGWQGRSRLLLWGVLPAGAAFLTALVLAPEPRNVVRAFT
                     YSAGGYEGSWGIVAFARLVSQASSALRPYDAAAPAAILAASLLLVPLFRRIPPWRAVL
                     LQILAFYLLAAGFGLQYLAWGLPFLALAGPRLALPFSAAATLHLSLAYVQGASKAFLH
                     GLRFPGNVWNDVHAAALTRVATVATSEGWDRLLLGTAAVLWLAILWTSIAAARPEPEG
                     GPLSVRA"
     gene            complement(8593..9369)
                     /locus_tag="P5164_00910"
     CDS             complement(8593..9369)
                     /locus_tag="P5164_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRY42477.1"
                     /translation="MKVALLQLDLAWEDVPANHARASRLLGQAADLGARLAILPEMFA
                     TGFSMDGRKVAQAEGGATETFLRERAASLGLHVLAGVAELAEPLPANRALLVSPDGSV
                     RRYTKIHPFSFAGEEKVMASGGTVVTWDVEGLRVTPLVCYDLRFPEPFRLAADDTDLY
                     AVLANWPERRRHHWSLLLRARAVENLAFVAGVNRVGDGDGLRYLGDSALVGPWGEAIV
                     SASEQETLLVGEVDAATVAEARRKFPVLADRRPGSYRRPG"
     gene            9471..11111
                     /locus_tag="P5164_00915"
     CDS             9471..11111
                     /locus_tag="P5164_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HRY42478.1"
                     /translation="MTAQKEQWASRAGLVLAMAGNAVGLGNFLRFPAQAAQNGGGVFL
                     IPYLVAFVLMGIPLLWVEWAIGRHGGQYGHHSAPGMLDRLGKSRLLKYIGVFGLFTNL
                     TVAAYYCYIESWTLAYVFHSLKGTFRSTPPTEFFPAYLGQHAGSITSLPPEATIWFVV
                     TLALNVWILSRGLAKGIEMAAKIGVPLLIVFGVVLVVRGLTLGTDAPGVVESPLTGLN
                     FVWNPDLSGLKDFNVWLAAAGQIFFTLSVGMGSIHCYAAYVKKDRDIALNAASAGWMN
                     EFVEVVIGGSLLIPIATAYLGLAAVKGATAGGSGFSMGFLTLPSLFNNWGWFAPVAGA
                     MWFGLLFFAGITSSLAMGQPILAFLADEFGAARAKGALLFGGCTFVLGMWCVWLYPGG
                     SFDEFDFWTGTFSLVVFALLEVIVFAWIFGMEKGWAEITRGADMKVPVVFRYVIPYVT
                     PLFLAVVFLGALVQPEGGDWGEAVRGLLAGKGWSLSAGSVLGKLMHVGDTGGWHGPNG
                     EVTPRMVKDLTRILLSLVFLTCGVLVYVAFRRKERRAS"
     gene            11108..11254
                     /locus_tag="P5164_00920"
     CDS             11108..11254
                     /locus_tag="P5164_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42479.1"
                     /translation="MTSAAWAMLGVTWTVITFFTVRFFLKVLRNPTPPEPGEDGPDIP
                     FKDA"
     gene            complement(11435..12046)
                     /locus_tag="P5164_00925"
     CDS             complement(11435..12046)
                     /locus_tag="P5164_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42480.1"
                     /translation="MAKTRPASGRARSKEEEYFLRAEAELLEKVRVRAAKEAERRAMG
                     EQHGVEDPEILAAFEEAGYDRHTVRILHLVPILQIAWVDGEISKAEREEILRLAAARG
                     VDEGSAAHARLLSWLDSPPSPEFFERTMTILARLIEALPEDREAAFQGEIMTSALKVA
                     SASGGFLGLGSKVSAEEKALLAHFAESFEKAHRAALEKAEKKG"
     gene            complement(12144..14597)
                     /locus_tag="P5164_00930"
     CDS             complement(12144..14597)
                     /locus_tag="P5164_00930"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRY42481.1"
                     /translation="MNPLCNARRRPGRFVLCAAVLPALLPAAPALASPRPLETVPPAS
                     VGLSATRLSRIDALLAEAIARQEVPGGVVLVGRHGKVAFRKAYGRRSVEPDVEPMTTD
                     TAFDLASLTKGVATAASVMTLVEEGRIALDDPVVRYLPEFGAGGGERERVTVEQLLTH
                     RGGLVADDPMSLYEGTPAEIYARKHRTPLATPPGLRFVYSDAGYEVLGELVRAVTGET
                     LDVYARRAVFDPLGMRETEFRPLGKEGRLPVGRIAPTEKRDGVFLRGEVHDPRARALG
                     GVAGHAGLFGTADDLARFARALLGDGDGWLSPAGIAATTRVRDYGDGDLRALGWDVET
                     HYSTSRGDLYPLGSFGHTGWTGTSMWLDPVTDSFVILLTARNHPGGAGNAIPLRTRLA
                     SVVAAAIRDVPADALRRASERFAVLTGRPPAPLRREPEAAPEALAFDVRTGIDVLESA
                     GFEAIAGRRVALLTNATGTTRDGRPTLSVLRSPRAKAAGVTVVRLLSPEHGLAAALDE
                     KVGDSVEPTSGLPVVSLYGEKRRPAPEDLAGLDALVVDLQDAGVRFYTYLTTMTYVME
                     ECAKAKVAVVVLDRPNPIGGLAVEGPPADSDRLSFVAVHTIPIRTGMTIGETARLLNA
                     EKKIGADLAVVPLRGWRRALWYDETGLPWVNPSPNLRSVTQAALYPGVALLETTNVSV
                     GRGTDAPFELVGAPWIDAERLARTLNGRGIPGVRFTPVTFTPASSTHAGALCHGLRIT
                     LTDRNALRPVALGLELATALRDLHGADWKRDRFGELLGSASAVTRFERGDPAGTIVAG
                     WAAEQMEFERRRARHLLYE"
     gene            complement(14594..16462)
                     /locus_tag="P5164_00935"
     CDS             complement(14594..16462)
                     /locus_tag="P5164_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRY42482.1"
                     /translation="MTHAERPLMGGRRTSGGPLAFLPALSLLLAFSACSSGRPGAGPS
                     DGLRAGPTLGHAPAVAALTLREKAAQVVMVGSRGVYRAEDDPEYLDLARLVSQEGVGG
                     VIWFLGTPLETADLNHRLNAKAKLPLLVAADLEAGPGMRFPDLVLGPSAMAVAATGDL
                     ALAARRARATAEAARALGIRVVFAPVADVNNNPDNPVINVRSFGEQPEAVGRFVAATV
                     AGLQEGGVLATLKHFPGHGDTSVDSHRSLPVLGVDRARLDAVELVPFRAGLAAGARGV
                     MTAHLAVPALDPEAAPVLPAPIRTKGFPEEVAELQTAGTMPAVVSRPITTTLLREELG
                     FHGLVFTDSMEMGGIVAHFEPGEAAVRVLEAGGDVVLMPPDPKAAIDAIVSAVETGRL
                     PAARLDEAVGRILAEKERLGLFRERSLPLHEIARAAGTREQKATEEEVARRGLTLVRE
                     AEGALPLREGARLLVLAVHDERTTVAIDATLQAELGKRAGSVDFVRLDPTSCAGEGAA
                     VAERAKEVDVVVLALFVRPRSGRGTLDVPAEGKAALAPLLASGRPVVGVSFGSPYLLR
                     DLPGLPTYLCAYGHQSLVQAAAVRALWGEAPIEGTLPVTIPGVAPRGMGIRKEAVK"
     gene            complement(16502..20824)
                     /locus_tag="P5164_00940"
     CDS             complement(16502..20824)
                     /locus_tag="P5164_00940"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRY42483.1"
                     /translation="MLRLRPFALLSGALLLAAATATASAPSAPPALDGPIPFSGKRNL
                     PNLVLLGARTERAHEKEATHEGRRYWEARRDWERLERAEIPAAPILRSVAQELYRERL
                     RFRTPDGGPQPLNATLAAAGWKSLGPTTDAGRVRDYAFTRDGSKLYVATANGGIWLLT
                     RQGGPGADYANPVNLTDDLPLLTFGAVAVAPSNPNVVYAATGEQSPLSGSQVIGLGTL
                     RSNDGGSTWSFNTTSVSGGAFDVIPSLYSYDLDVHPSNPEDVVLGTANGIFRSTDGGR
                     TWVNRLPATGEGLEWRRQGVNIARSPANPNVLWAGLWGGLAFSTDGGDTWQVAFEDIA
                     QQAGFNGIPIRSLVAISPSNPNRIYWLVAGYDLQRGYSQVGVFRSDNGGQNWGTALGP
                     PAGQGYPLIAGTQGWTFLGLAVDPTNPDRLIAGGLDTWRSEDGGLSWTQISQWTLPER
                     HPQFCHADVNVIAFEPGRSSFWMGTDGGLFRSTNGGRSFAWKNDGVVARMFSSLAQHP
                     TDPYRLYAGTQDNGTMRLSGDSTTAWKRIFYGDGYDCAVNPQNPSIVYATNFNGYTSR
                     ADDGGESEESFRLTTCPADAQTEDQCSVPPVTSFRSRLAIDPVDPRVLYTMTDRIYRT
                     SNGAATPSDWQPVFAEYFCSDGVSAQPCPNVQKSWASCSSISIRPTDRNRVAFGTAAG
                     YVVYTLNGFQNGSLVNIGSQVNAIAWDPADPNAFYVGLESATEIVAGQGRHVIWRIAG
                     LNQQQQTASPASSGIGVPITYAGGSFTFFAPVDSLVFSPTDPSLMFAGTKYGIFRSTN
                     KGQSWGRFGDDFPATWVSYLLFSPDGRRLRAATWGRGMWEINPAGGTPSPTSPPTPDF
                     SFTPAQPKPGQSVSFSDRTQGGAATWSWSFGDGTTSTAQNPTKVWGSPGTYSVTLTAA
                     NAAGSRSVSKGVTVSYGSTGTGSVYTYLLPIVLTSSGAGGAFFTSELTLTNRSSRTLN
                     LTFRAKGSFESSSTYSLPPGQQVHPDVFAFLQSATGMGVPAGNKTASLRIEVAGADNL
                     VQFGAQVRVTTPATADLRAKGVIGRFGLAFPATPLGRAAQTEAFVFGLQQTSAAGSAG
                     TRSNLACVNAGGGAGGAVRLEATYYNGDTGAAHASKDLFDLAPFQWEQKSTPLAARGL
                     RYGYAVVRRTSGSDQFVCYAVLNDNLNGDGAFVPMVPNDSPSRTSAALVPVVLEAAGY
                     TSEMTFANRTNRDISGLFALVPRTDPVPDWGYFDLPARSQFTVPNVVAELRRIGFTAP
                     AGTVASVFAQFLDGQFREDQSDTQAPIPTSDGFLGVRTTTARAGGLLGLAYGYTPIGE
                     AADTEAWVYGLQQTGTRGQEGGTRANLAVGHALGGQVEPLQLEVTYFGPNGNELGKEP
                     QCSPCTLAPGQFLQFDSALARYGVPHGYARIRRLAGTDQFLAYGVLNDQGNDDGSYVP
                     MIVP"
     gene            complement(20997..22301)
                     /gene="lysA"
                     /locus_tag="P5164_00945"
     CDS             complement(20997..22301)
                     /gene="lysA"
                     /locus_tag="P5164_00945"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRY42484.1"
                     /translation="MTLREGLLRVRAGEAAHPEAVDALTRLAADHGTPLYVYDAETIR
                     EKARALRAAFEPRFPRLGLRYALKANTNVEIVKRVLAEGLAPEVVSEGEVRAALRAGA
                     AGRDVLFTSSSKSPSEIDFALRHDVLLNVDNLDELAQVSAAALRLGTTARISFRINPG
                     VDPDTLHQINTGIPESKFGVHLDGGHARAAYALARALPALAITGVHCHIGSQITETAG
                     YEKTARRMLAFVRELKEELGLTLSFVDLGGGLGIPFADGQAVMTPEDLARALKPIWDE
                     EVELLGYEPELWIEPGRYLVAQAGLMVARVNSVKTTPVKTFVNVDAGFNTLMRPALYG
                     AAHRVRIVGRDGEPMLLDVAGDVCETGDILAEGRLLPRPSAGDLVAFLDAGAYGFVMA
                     SEYNARPLPAEVLVDGDAVRVIRKRGTWEELHRSEPEVGPPL"
     gene            22449..23513
                     /locus_tag="P5164_00950"
     CDS             22449..23513
                     /locus_tag="P5164_00950"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HRY42485.1"
                     /translation="MRTRRSLLSEAALASAFLAFPRSARAAAPPVAPAPLRPKALAPG
                     SRVALVSPASPGAEAESAVIAEEVVASLGLVPRRMPAAARQTMYLAGTDEERAADLNA
                     AFRDPSIDAVWCIRGGYGSGRLLPLLDWEAIRRNPKPLLGFSDITALLNGFYARTGLV
                     TFHAPTCSENLSDYTLAELKRVVFTPEAAGVVAAAPPVEPKEGFVDREDRLRRIVPGT
                     GRGRLVGGNISVFSMLVGTPWEPPLAGSILFLEEVGEEPYRIDRWLTHFVLTGRLANC
                     AGIVFGKFKDCGPGDEGSYQGTWTWQEVVADRLGKLGVPVLAGLRFGHVADKSTFPVG
                     VLAELDVAAGTLSLLEPAVS"
     gene            23510..>23870
                     /locus_tag="P5164_00955"
     CDS             23510..>23870
                     /locus_tag="P5164_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42486.1"
                     /translation="MREGRRARRAGRAACALLLALFVAPPIAAAEPSRAPAGLPLVLD
                     TGWRAADADPPDGIAGIDRLDFRPADPVSDQARRGEVRWYRVLVDLSPFVGQPLAFAA
                     DGLRDVDEAWFDGVPIGG"
BASE COUNT         3293 a         8313 c         9002 g         3262 t
ORIGIN      
        1 ttcaccggcc agccgaagct gcgggagacg ctgggcgtct tcctgacggc cgcgcggaga
       61 cggaacgagc cgctcgacca cgtccttctg ttcggcccgc ccggcctcgg gaagacgacc
      121 ctcgcccaca tcatcgcgag ggagatgggg gcccagctcc gcgtcacggc gggccccgtc
      181 ctcgagaagg ccggggacct cgcggcgatc ctcacgaacc tcggcccggg agacgtgctc
      241 ttcatcgacg agatccaccg cgtgcccccg gccgtcgagg agatcctcta tcccgcactc
      301 gaggacggga agctggacct cgtcatcggg acggggcccg cggcgcggac ggtggagctg
      361 cgcctgcagc cgttcacgct ggtcggcgcc accacgcgcg cggggctcct ctcgcagccg
      421 ttgaccgggc ggttcggcat cacgcaccgc ctcgactact acgacacggc cagcctcagg
      481 tcgatcgtcc tgcgctcggc cgagatcctc ggctgcccga tcgacgagga cggcgcgggc
      541 gagatcgccc ggcgcagccg cgggacgccg cgcatcgcga accggctcct ccgccgcgtg
      601 cgagacttcg tcgaggtcgc gggggagagc acgatctcgg cggccggcgc gcgcgcctcg
      661 ctcgaccggc tcgaggtcga ccacttcggc ctcgacgagc tggaccgccg gatcctcggg
      721 acgatcgtcg agcgcttcgg cggaggaccg gtcggccttt cggcgctcgc gcactccctc
      781 ggcgaggaca aggggacgct cgaggacctc tacgagccgt acctcctcca gtcggggttc
      841 ctgacccgga cgccgaaagg gcgcgtcgcc acggcgctcg cctaccggca cctcggcctc
      901 ccgcaggccg cgtcgtcgac cgggccgctc ttcgacggga ccccgtgagg cgcggcctcc
      961 tcgtctacaa ccccgcggcg ggagggcgcg accggacggc ccagatggcc gcgatcgcgg
     1021 accgtgcccg gtcgaaaggc gtcgagctcg ccctcctcgc gacagagggg ccggggcacg
     1081 cgacggagat cgtccggctg cgcctcgcgg agcgtcccga cctcgtcgcg gtcgccggcg
     1141 gggacggaac ggtcggcgag gcggctgcgg ccctcgtcgg gagcgacgtc ccgctcgcga
     1201 tcctccccgc ggggaccgcg aacgtcgtcg cccgcgagta cggggtcggg atcacaccgg
     1261 aagaggcgga ggcggtcctc gggtcgacga cgacgcggac gctcacgacg tggccctcgg
     1321 cggggaggac ctccctcatc gggacgggag tcggtttcga cgcgcgggtc atggcgaacg
     1381 tgaacccgct gctcaagaga ctcttcggcc gtcccggctt cgccctcacc tcgaccctcg
     1441 agtggtaccg gtacgagttc cctccgatcg aggtgaccgg cctcgacgcc gacggtcggc
     1501 cgttcgtgcg gcgcgcgacg ttcgtccttt cggcgaacac gcgaaagtac gcgggcgacg
     1561 cgatcctctc cccgttcgcg gaccccgacg acgacctcct cgacctcgtc ctcttcacgt
     1621 cggaggaccg gggagacctc ttccagttct accgcctcct ccccggcggg cgcgcggcgc
     1681 agctcggtgt ccgcggcgtc tcgcggctcc cggtccggag cttcacggcg cgctccctcg
     1741 cgggctacga gctggaggtc caggtggacg gggacgcggc cgggacgacg ccggtgaccg
     1801 tcggtcccgc gggcgggaag gtccggatcc tcgtcccgtc ccccgcgcgg gcctgaccgc
     1861 gcgacgccgc gtccggggca cgccggtgac gcgcgtcaca gggttggccc cgtggccggg
     1921 cgaggccaga atccgggcat gagagtcgcc ggtgccgtcg tcgccgcctg cgccctgtgg
     1981 ggcgcggccc tcctcgggac cggcccggcc tcggccggcg tcgagccggt cctcgtcgcc
     2041 tccggcttca ccgcgccgct gcgcgtggcg gcgcccgccg gcgacttctc ccgcctcttc
     2101 gtcgtcgagc agggagggag agtccggatc gtgaaggacg gggtcctcct cggagggagc
     2161 ttcctcgacc tctccgggcg gatctcgagc ggaggcgagc gcgggctcct cggcttcgcg
     2221 ttccaccccg agtaccaccg gaacggccgc ttcttcgtca actacaccga cccgaccggc
     2281 cggacggtcg tctcggagat gagggtctcg gccgaccccg accgtgccga cggcgggagc
     2341 gagcgcgtcc tcctgcgcca ggcgcagccc tttgcgaacc acaacggcgg gcacctcgcg
     2401 ttctcgcccc tcgacgggac gctctggatc ggcctcggcg acggaggggg cggcggcgac
     2461 ccgcagaaca acgcgcagag tgacgggacc tggctcggaa agatgctgcg catcgacgtc
     2521 gacgcgaagg acccgggaaa ggagtacgcc gtcccttccg acaacccgtt cgcggggcgg
     2581 acgactccgc ttcccgagat ctgggcgaag gggctccgga acccgtggcg ctattcgttc
     2641 gaccgggcga cgggcgacct cttcgtcggc gacgtcggcc agggaacctg ggaggaggtg
     2701 gacttccagc cggcctcctc gagaggaggc gagaactacg gctggcggcg aatggagggg
     2761 agccgctgct acgacccggc gaccggctgc cagaccggct cgctcgtcct gccgatcctc
     2821 gagtacgagc acgccgggag cccgcggcgc tgctcggtga cggggggtgt cgtcgcgcgc
     2881 gggccgtcgg tgcccgagat cgcggggcgc tacctcttcg ccgactactg ctccggcgag
     2941 ctcctgtccg tgaaggtcct cggcggagcg gccaccgacc cgcgcaacca ccgctcggag
     3001 tggatgccgt cggggggccg ctccctcgcg accgtgacgg acatcggcga ggacgcgcgc
     3061 ggcgacgtct acgtcacgga cgcctccggc ggggaggtct ttcgcctgac gacgacggcc
     3121 cccacggcct cccgcctcgt cccgatcgtc ctcgacgcca gcggaaggaa cggctcgcgc
     3181 tacggcaccg agctcgtcgt cgcgaatcgt ggcgcccgca ccgccacgat ccgggcgaag
     3241 tacacgccgg ccgcccgttt cggcggggcg ggcggcggga gcgtcgcgga gatcctcggg
     3301 cggggcgagc agaggaccta ccccgacgcc ctcgcctggc tccggacgaa ggggctcccg
     3361 atccccgacg cgtccgccgg gtctcagggg gggacgctcg aggtcgagct gacgggcctc
     3421 gcgtccgctt ccgacggcgt cgtcctcgcg cggacgacga ccccttccgg caccggccgg
     3481 gcgggcctcg cctaccccgc gccgcgggcg caggacctcg cgacggagga ggccgtcctc
     3541 tacggcctcc gggagaacgc gtcggaccga acgaatctcg cgctcctgtc gggcgcgacg
     3601 accgggacga cgacgcttcg cgtcacgctc gtctcgggcg cgcgctcgtt cgtcctgccg
     3661 gaggcggtcg cgctcgagcc cggggaatgg ctccagctcg actcggtcct ggcgcgcgcc
     3721 ggcttctcga gcggctgggc ggtcgtggag cgcgtctcgg gcccgggccc gttctgggtc
     3781 tacgccgtct tcaacgacgc cggcacgaac gacggctcct acgtggcgcc gagccggccg
     3841 gtccgccccg cccagaccca ggtcgtcccc gccgtcgtgg agacgccggc gttcgagagc
     3901 gagctcgtcc tcgcgaaccc gggaccgtcg cccgtcaccg caaggctcct ctgggtggag
     3961 acgctcgcga ccggcaagcc gaggaggttc gaggccgccg agccgctggc ggccggggag
     4021 cagaagatcc tcgcctcctt cgtcgaccgc ctgcgcgcac gcgtcccgtc gatcggcgcg
     4081 cgcggggggc ggtactccgg cgctctcttc gtcacgttcg agacgcccgc cggacggaag
     4141 gctccgggct atgcgggagc gcggaccgcc gcgcccgccg acggaggagg cgcctacggc
     4201 ctcttcacgc ccggatcggg gctcgccgag tgcgcgttcc gcgaagcgtg gctcttcggc
     4261 ctccgtcagg acgactcggc gaggacgaac ctcgccctcg tgaacgtcga gcccgacggc
     4321 ggcccgatca cgctccggtg ggaggccttc gacgcgaacg ccggccggcg catcggcacc
     4381 tcccgcgacc tcgtcctggg gcccggagag tggcggcagg tggacggcgt cctcggcgac
     4441 ttcctcctcc gggccggcta cctgaaggtc tcccgcgtct ccgggaacgg ccggttcctc
     4501 gcctacgccg tcctgaacga cggtccggcc ccgggcgggc ccgggacgca cgacgggagc
     4561 tacgtgccga tggaggcggc ggagtagggg aggtcagccc tctccgaccg agaacgacgc
     4621 ctccgcgtcc tgagaggcga ggaagaagca ggagcgggag acgtcgacga gctcctcctg
     4681 cagcgcctcg agccgctcga ccctctcctt cggccccggc gcctcgagcg ccgcgaagaa
     4741 ctcgggggcg ctccccgaga agtggatctc ggcgagaccg tcgaacggcg gcgccgagcc
     4801 ccgccgggcc tgccactcgg cattcgcacc gacggcgagc gcggcgctca cctcgacgcg
     4861 caccgcgccg agctcggcgc cgagggagac gatctcctcg ccgtaccgct gccactggcg
     4921 gcggaactcc acgacggtca ggtcggggcg gcggcggacg cactggaccc acttgagcac
     4981 ggtcggcctc ctcgcgaggg cgctggagcg aaacgaatca tagccgcccg cagcggccca
     5041 cacccgatcc cggagcgcgg aagctcagct cctcaggttc cgcatgacga gcgcgaagca
     5101 ggcgaccgcg agggcgagga ggccgagtcc cgccgcggtg gcgccgagga cgagcttccc
     5161 gatcccgaag agggcggcgt agacgaggac ggcggaaagc gcccaattcg cgagcgagag
     5221 cgccccgccg ggaatcggct cgcgaccgaa gcccatcgac gccgagacgc gggcccagcc
     5281 gggtccgccc ggtcggaccc tcccgtagaa cgaacggagc ttctcgtcgg gctccggctt
     5341 cgtcaggtac gtgacggtga cccagacgat cgtcgaggcg acgaccgtca cgagcatcgt
     5401 cttcgcctgc gcctcgaagt ccgcgccggg tccgggcgcg atcgctccgg ggcgtgcgaa
     5461 ccacgccatg gcggcgaggg agacgacgcc ggaggacccc atcgccgaga tctcgctcca
     5521 cgcgttgatc cgccaccagt accaacgcag gatcaggacg agcccggtgc cgctcccgag
     5581 cgcgaggagg aaccgccacg ccccttcgat cgtgccgagg ttcagcgtca cgacgatcga
     5641 ggcgaggaag aggaggaccg tggtcaggcg cccggcgtgg acgtagtggc tctcgctcgc
     5701 tcccggtctc aggaaccggc ggtagacgtc gttgacgagg tagctcgccc cccagttcag
     5761 gtgggtcgcc atcgtggaca tgtaagcggc tgcgaagccg gcgaggagga atcccttcca
     5821 gcccgacggg aggaggtcga ccatcgcctg cacgtaggcc gcctcgacgt ccctcttccc
     5881 ggcgatctcg acgccgcccg ggtagaggag gacggtgcag aggcccgtca cgatccaggg
     5941 ccagggccgg agcgcgtagt gcccgacgtt gaagaagagc gtcgcgagga ctccgtcccg
     6001 ctcgctccgc gccgagaaga tccgctgcgc gacgtagcct cctcctcccg gctcggcgcc
     6061 cgggtaccag gcggcccacc actggaccgc gaggaacgtg aagagcgcga tcgcgggcat
     6121 ccagcgtgag ccggcggggg ggacgacgga gagcgccgcg tcgagcgagc cgaagcgtcc
     6181 tgcgagcccc tccttcagcg cgcccatgcc gccgacggcg cggagggcgt agacggcgag
     6241 gacgatgacg gccgtcatct tgatgacgag ctggatcagg tcggtccaga ggacggccca
     6301 catcccggcc gccgcggagt aggcgacggt aatgacgaag cagatgccga cggcccagac
     6361 cggcgagatc ccgagcgcga tgccgaggat cttcaccatc gcgagcgtga cccagcccat
     6421 gatgatcagg ttgatcggaa gcgcgaggta gatcgcccgg aagccgcgga ggaacgtcgc
     6481 cgggcggccc gagtagcgca cctcggcgaa ctcgacgtcg gtcatgacgt tcgcgcgccg
     6541 ccagagccgg gcgtagagga agacggtgag cgtcccgctc aggaccatgt tccaccagag
     6601 ccagttcccg gcgacgccgt gcttcgcgac gaggcccgtc acgacgagcg gcgtgtcggc
     6661 cgcgaacgtc gtcgcgatca tcgccgcgcc cgccagccac cacggcgcgg agcgcccggc
     6721 gacgaagtac tcctcgagcg attcgccccc cttcttcgtg aaggcgaggc cgaccccgat
     6781 cgacaacgca aagtaggcga ggacgattgc ccagtccaga agcgtcaggc tcatgacggt
     6841 cgctcatctt agcggtccgg gactttttcc ttgccaccgc cgcggcgcgg cacgatcatg
     6901 cgcggcgtgg aggacgctgc gtgtccgggc gcgagtcggc cgtcgagacg cggctctcct
     6961 cgatcctcgc ccgcggccgt gtcgagaccg gcttctcggc gctgagcccc gaggacgccg
     7021 tcgagcggct cctccggccc gttctcctcg cggagggctt ctccgcggac gccgcggacg
     7081 gcgcgctcga ggggatccgt aatcgcgagg gggccggatc gacctcgttc ggcgccgtgg
     7141 ctcttccgca cgcgcgggtc ccgcgcctcg gccggatcgt cggcgcgctc ggcgtgaacg
     7201 gcgagggcgt ctacgcctcc ggcccgcagg ggatccgggt cgtcctcgcc ttcgcctctc
     7261 ccgcctccgc gaccgtcgag cacctccagt tcctcgcgca ggtcgcccgg ctccttcgcc
     7321 aggaggagac cgtcgccgac ctcctcgagg ccaccgacgg cgccgcgctc gtctccgcga
     7381 tccggcggcg ggagcgctag gcgcgcacgg agagagggcc cccttcgggc tccgggcggg
     7441 ccgccgcgat cgaggtccag aggatggcga gccagaggac ggcagccgtg ccgaggagga
     7501 gccggtccca gccttcgctc gtcgcgacgg tcgcgacgcg ggtcagggcc gcggcgtgga
     7561 cgtcgttcca gacgttcccg gggaagcgga gaccgtggag gaacgctttc gaggccccct
     7621 ggacgtacgc cagcgagagg tggagcgtcg ccgccgcgga gaaggggagc gcgagccggg
     7681 ggcccgccag ggcgaggaag ggaagccccc aggcgaggta ctggaggccg aagccggccg
     7741 cgaggagata gaaggccagg atctggagga ggacggctct ccagggaggg atgcgacgga
     7801 agagcgggac gaggaggagg gaggccgcga ggatcgcggc gggcgccgcc gcgtcgtacg
     7861 gccggagagc cgacgaagcc tgcgagacca ggcgcgcgaa ggcgacgatc ccccagctcc
     7921 cttcgtagcc gcccgcggaa taggtgaagg cccgtacgac gttccgcggc tccggcgcga
     7981 ggacgagcgc ggttaggaac gccgcgccgg ccggaaggac gccccagagg aggagcctgg
     8041 aacggccttg ccaccctctc gccgaggcga gaagcgcggg tgcccagagc gcggggacct
     8101 tcttcaggac gatcgcgccc gcccaggcca ggcccgcgct tcgtccggag gtctcgcggg
     8161 ccaggaggag agcggcgcag aggacgagcg ccgtgtggag cgagtcgaag ttcccgtggt
     8221 agcaggtcgt cagaagcgag acgggattcc accagaagag gaggaacggg cgtccatcgg
     8281 acggacgggc cgcgcgaagc gcgccggcgg agaggaggtc cccggcaatc gccgggagct
     8341 tcacggcgag ctggaggggg aggcccgcga gccccgagaa gaggtagagg acgacggcgt
     8401 agagaacgac gacgaccggg tggaagtgat agacgcgacc gccgtcggcg tagagcgccc
     8461 agaggccgtc gtgctggaag atgccggcga agcgcacgag cgtgtagacg tcgtacgagc
     8521 cgttccagaa caaggccagg gcgaggcgca ggccgagccc ggcgaggagg tgcgcgagga
     8581 agcgccggcg cgtcacccgg gccgccggta gctgccgggc ctgcggtcgg cgaggacggg
     8641 gaacttcctc cgtgcctcgg cgacggtcgc cgcatcgacc tccccgacga ggagcgtctc
     8701 ctgctccgag gcggagacga tcgcctcgcc ccagggtccg acgagggcgg agtcgccgag
     8761 gtaccggagc ccgtccccgt cgccgacgcg gttgacgccg gcgacgaagg ccaggttctc
     8821 caccgcgcgg gcgcgcagga ggaggctcca gtgatggcgg cgccgctcgg gccagttcgc
     8881 gaggacggcg tacaggtcgg tgtcgtccgc cgcgaggcgg aacggctcgg ggaagcggag
     8941 gtcgtagcag acgagcggcg tcacgcgcag tccctcgacg tcccaggtga cgacggtgcc
     9001 gccgctcgcc atcaccttct cctcgccggc gaacgagaag gggtggatct tcgtgtagcg
     9061 gcggacggag ccgtccgggg agacgaggag ggcgcggttc gcagggagag gctccgcgag
     9121 ctcggcgacg cccgcgagga cgtgcaggcc gagggaggcc gcccgctcgc ggaggaacgt
     9181 ctcggtcgct cccccttcgg cctgcgcgac cttccggccg tccatcgaga aaccggtcgc
     9241 gaacatctcc gggaggatcg cgaggcgggc gccgaggtcg gcggcctggc cgaggaggcg
     9301 ggaggcgcgc gcgtggttcg ccgggacgtc ctcccaggcg aggtcgagct ggaggagggc
     9361 gaccttcatg gggcggattc tgccgcggtc ggatcgcgat gagtccgttt ctcgcgttga
     9421 cgccccctcc cgggcgcggc taccctgccg ggtcggcaga gggggtgccc atgacggcac
     9481 agaaggaaca gtgggcttcg agggcggggc tcgtcctcgc gatggccgga aacgccgtcg
     9541 gcctcggcaa cttcctgcgc ttcccggccc aggcggcgca gaacggcggg ggcgtcttcc
     9601 tgatccccta cctcgtcgcg ttcgtcctga tggggatccc gctcctctgg gtcgagtggg
     9661 cgatcggccg gcacggcggg cagtacgggc accactccgc ccccgggatg ctcgaccgcc
     9721 tggggaagag ccggctcctg aagtacatcg gcgtcttcgg cctcttcacg aacctgacgg
     9781 tcgcggccta ctactgctac atcgagtcgt ggacgctcgc gtacgtcttc cactcgctga
     9841 aggggacgtt ccggtcgacg ccgccgacgg agttcttccc ggcctacctc ggacagcacg
     9901 ccgggtcgat cacctcgctc ccgccggagg cgacgatctg gttcgtcgtc acgctcgcgc
     9961 tgaacgtctg gatcctctcc cgcggcctcg cgaaggggat cgagatggcc gcgaagatcg
    10021 gcgtcccgct cctgatcgtc ttcggcgtcg tcctcgtggt gaggggcctc accctcggca
    10081 cggacgcccc gggcgtcgtc gagagcccgc tcacgggcct caacttcgtc tggaacccgg
    10141 acctgtcggg gctcaaggac ttcaacgtct ggctcgccgc ggcgggtcag atcttcttca
    10201 cgctctcggt cgggatgggc tcgatccact gctacgcggc ctacgtgaag aaggaccgcg
    10261 acatcgccct gaacgccgcg agcgccggct ggatgaacga gttcgtcgag gtcgtcatcg
    10321 gcgggtcgct cctgatcccg atcgcgacgg cctacctcgg tctcgcggcc gtgaaggggg
    10381 ccacggccgg cggaagcggc ttctcgatgg gcttcctgac gctcccgagc ctcttcaaca
    10441 actggggctg gttcgcgccg gtcgccggag cgatgtggtt cggcctcctc ttcttcgccg
    10501 ggatcacgtc gtcgctcgcg atggggcagc cgatcctggc gttcctcgcc gacgagttcg
    10561 gcgcggcgcg cgcgaagggg gcgctcctct tcggcggctg caccttcgtc ctcgggatgt
    10621 ggtgcgtctg gctctacccg ggcggctcgt tcgacgagtt cgacttctgg acggggacgt
    10681 tctcgctcgt cgtcttcgcc ctcctcgagg tgatcgtctt cgcctggatc ttcgggatgg
    10741 agaagggttg ggcggagatc acgagagggg ccgacatgaa ggtgcccgtc gtcttccgct
    10801 acgtcatccc ctacgtgacc cccctcttcc tcgcggtcgt cttcctcggg gccctcgtcc
    10861 agcccgaggg aggggattgg ggcgaggcgg tccggggcct cctcgccggg aagggctggt
    10921 cgctgtcggc cgggagcgtc ctcggcaagc tgatgcacgt cggcgacacc ggaggctggc
    10981 acgggccgaa cggcgaggtg acgccgcgga tggtcaagga cctgacccgg atcctgctct
    11041 cgctcgtctt cctgacctgc ggggtcctcg tctacgtggc cttccggcgg aaggagagga
    11101 gagcgtcatg acctcggccg cctgggcgat gctgggggtg acctggacgg tcatcacttt
    11161 tttcacggtc cgctttttcc tgaaggtcct ccggaacccg acgccccctg aaccgggcga
    11221 agacgggccg gacatcccgt tcaaggacgc ctgacccgac gggcgagccc cgcgaaggtg
    11281 cccccccccg ctaaggtgcc aggcgtgaaa tcgtgtattg tttgaaacaa tacacgattt
    11341 cacgcctggc acctggttcg gtggcacctg gttcgggacc ccggatgcgc cgacctctct
    11401 cagctcgcgg ggcggcgtcc gggggcgcgg agactcagcc cttcttctcg gccttctcga
    11461 gggcggcccg gtgggccttc tcgaagctct cggcgaagtg ggcgaggagg gccttctcct
    11521 ccgccgagac cttgctgccg agcccgagga aaccgcccga cgccgaggcg accttcaggg
    11581 cggaggtcat gatctcgccc tggaaggcgg cttcccggtc ctccgggagc gcctcgatga
    11641 ggcgggcgag gatcgtcatc gtccgctcga agaactccgg ggacggaggg gagtcgagcc
    11701 aggagagaag gcgggcgtgg gccgcgctcc cttcgtcgac gccgcgcgcc gcggcgagcc
    11761 ggaggatctc ttcccgctcc gccttcgaga tctccccgtc gacccaggcg atctgcagga
    11821 tcgggacgag gtggaggatc cggaccgtgt ggcggtcgta gccggcctcc tcgaacgccg
    11881 cgaggatctc ggggtcttcg acgccgtgct gctcgcccat cgctcgccgc tcggcctcct
    11941 tcgccgcgcg gacgcgtacc ttctcgagca gctcggcctc cgcccggagg aagtactcct
    12001 cttccttgga gcgcgctcga ccggaagcgg ggcgggtctt ggccatctgg ggtcctcctc
    12061 gtcgaagctc gggtctgaat ccgcggcggg gttttaccag aacgcccggc ccgagggcga
    12121 ggggcggggc gaccgggacc ggtctattcg tagagcaggt gtcgggccct gcggcgctcg
    12181 aactccatct gctccgcggc ccagccggcg acgatcgtac cggccgggtc gccccgctcg
    12241 aaacgcgtca cggcggaggc gctgccgagg agctcgccga atcggtcccg cttccagtcc
    12301 gccccgtgga ggtcgcggag cgcggtcgcg agctcgaggc cgagcgcgac gggccggagc
    12361 gcgttccggt cggtcagcgt gatccggagc ccgtggcaga gagcgccggc gtgcgtggag
    12421 gaggcgggag tgaacgtgac cggtgtgaag cggaccccgg ggattccccg tccgttcagc
    12481 gtccgggcga ggcgctcggc gtcgatccac ggggcgccga cgagctcgaa cggcgcgtcc
    12541 gtgccgcggc cgacggagac gttcgtcgtc tcgaggaggg cgacgccggg atagagcgcc
    12601 gcctgcgtga cggagcgaag gttcggcgac ggattcaccc aggggaggcc cgtctcgtcg
    12661 taccagagcg cgcggcgcca gccgcggagc gggacgacgg cgaggtcggc gccgatcttc
    12721 ttctccgcgt tcagaagccg ggccgtttcg ccgatcgtca tgccggtccg gatcgggatc
    12781 gtgtggaccg cgacgaagga gagccggtcg gaatcggcag gcggcccctc cacggcgagc
    12841 cccccgatcg ggttcggccg gtcgaggacg acgacggcga ccttcgcctt cgcacactct
    12901 tccatcacgt aggtcatcgt cgtgaggtag gtgtagaagc ggacgcccgc gtcctggaga
    12961 tcgacgacga gcgcgtcgag gccggcgagg tcctcgggcg cggggcgccg cttctcgccg
    13021 tagagcgaga cgaccggaag gccggaggtc ggctcgaccg agtctccgac cttctcgtcg
    13081 agggcggccg ccagcccgtg ctcgggcgag aggaggcgga cgaccgtcac gccggcggct
    13141 ttcgcgcgtg gagagcggag gacggagagc gtcgggcgcc cgtcgcgcgt cgtccccgtc
    13201 gcgttcgtga ggagcgccac gcgccgtccc gcgatcgcct cgaagccggc cgactcgagg
    13261 acgtcgatcc cggtccggac gtcgaaggcg agcgcctcgg gggccgcttc cggctcgcgg
    13321 cggagagggg ccggcgggcg gcccgtcagg acggcgaaac gctcggaggc gcgccggagc
    13381 gcgtcggccg ggacgtcccg gatcgccgcc gcgacgacgc tcgcgaggcg ggtccggagc
    13441 gggatcgcgt tcccggcgcc gccggggtgg ttccgcgcgg tgaggaggat gacgaagctg
    13501 tcggtgaccg ggtcgagcca catggaggtc ccggtccacc cggtgtgccc gaaggagccg
    13561 agcgggtaga ggtcgccgcg gctcgtcgag tagtgggtct cgacgtccca cccgagcgcc
    13621 cgcaggtccc cgtccccgta gtcgcggacg cgggtcgtcg cggcgatccc ggcgggagac
    13681 agccagccgt cgccgtcgcc gaggagggct cgggcgaagc gggcgaggtc gtctgccgtg
    13741 ccgaagaggc cggcatgacc ggcgacgccg ccgagggcgc gcgcgcgcgg gtcgtgcacc
    13801 tctccgcgta ggaagacgcc gtcccgcttc tcggtcggtg cgatccggcc gacgggaagc
    13861 cgtccctcct tcccgagggg ccggaactcg gtctcgcgca tgccgagcgg gtcgaagacc
    13921 gcgcggcggg cgtaaacgtc gagcgtctcg cccgtcacgg ctcggacgag ctcgccgagg
    13981 acctcgtagc cggcgtcgga gtagacgaag cggagcccgg gcggcgtcgc gaggggtgtc
    14041 cgatgcttgc gcgcgtagat ctcggccgga gtcccttcgt agagcgacat cgggtcgtcg
    14101 gcgaccaggc cgccccggtg cgtcaggagc tgctcgacgg tgacgcgctc ccgctctcct
    14161 ccgccggcgc cgaactcggg caggtaccgc acgacggggt cgtcgagcgc gatccgcccc
    14221 tcctccacga gggtcatcac gctcgcggcg gtggcgaccc ccttcgtgag agaggcgagg
    14281 tcgaaagccg tatccgtcgt catcggctcg acgtcgggct cgaccgatcg gcggccgtac
    14341 gccttgcgga aggccacctt tccgtgccgg ccgacgagga cgacgccgcc cgggacctcc
    14401 tggcgcgcga tcgcctcggc gaggagcgcg tcgatacgcg agaggcgggt cgccgagagc
    14461 ccgaccgacg ccggcggcac cgtctcgagg gggcgagggg acgcgagcgc gggcgcggcc
    14521 gggaggagag ccgggaggac agccgcgcag aggacgaagc gtcccggccg gcgccgggcg
    14581 ttgcagagcg ggttcacttc accgcctcct tccggatacc cattcctcgc ggagcgacgc
    14641 cggggatcgt gacggggagc gtcccctcga tcggggcttc cccccagagc gcgcggacgg
    14701 ccgccgcctg gacgagagac tggtgtccgt acgcgcagag gtaggtcggg agaccgggga
    14761 gatcgcggag gaggtagggg cttccgaacg agacgccgac gacgggccgg cccgacgcga
    14821 ggagaggcgc gagcgcggcc ttcccctccg ccgggacgtc gagcgtgccg cggccggagc
    14881 ggggcctcac gaagagcgcg aggacgacga cgtcgacctc cttcgcccgc tccgcgaccg
    14941 cggctccctc tccggcgcag gaggtcggat cgaggcggac gaagtcgacc gagcccgcgc
    15001 gcttcccgag ctcggcctgg agcgtcgcgt cgatcgccac cgtcgtccgc tcgtcgtgga
    15061 cggcgaggac gaggagccgg gcgccctcgc ggagcgggag cgcgccttcc gcctctcgga
    15121 cgagcgtcag gccgcggcgc gccacctcct cctccgtcgc cttctgctcc cgcgttccgg
    15181 cggcgcgggc gatctcgtgg agcgggaggc tccgctcgcg gaagaggccg agccgctcct
    15241 tctccgcgag gatccggccg acggcctcgt cgagccgggc cgcgggcagg cggcccgtct
    15301 cgacggccga gacgatggcg tcgatggcgg ccttcgggtc gggcggcatc aggacgacgt
    15361 ctccccccgc ctcaaggaca cggacggcgg cctcccccgg ctcgaagtgg gcgacgatcc
    15421 cccccatctc catcgagtcg gtgaagacga ggccgtggaa gccgagctct tcgcggagga
    15481 gcgtcgtcgt gatcgggcgg gagacgacgg cgggcatcgt gccggccgtc tgcagttccg
    15541 cgacctcctc ggggaagccc ttcgtccgga tcggggccgg gaggacgggc gccgcctcgg
    15601 ggtcgagcgc gggaacggcg aggtgcgccg tcatgacgcc ccgcgccccc gcggcgagcc
    15661 cggcccggaa cgggacgagc tcgacggcgt cgagacgggc acggtccacg ccgaggacgg
    15721 ggagcgagcg gtgcgagtcg acgctcgtgt cgccgtgccc cgggaagtgc ttcagcgtcg
    15781 cgaggacgcc cccttcctgg agccccgcga ccgtcgccgc gacgaagcgc ccgaccgcct
    15841 ccggctgctc gccgaagctg cggacgttga tgaccgggtt gtcggggttg ttgttgacgt
    15901 cggcgaccgg agcgaagacg actcgaatgc cgagggcgcg cgccgcctcc gccgtcgcgc
    15961 gggcccggcg tgcggcgagc gcgaggtcgc ccgtcgccgc gaccgccatc gccgacggcc
    16021 cgaggacgag gtcggggaag cgcatcccgg gccccgcttc gaggtcggcc gcgacgagga
    16081 gcgggagctt cgccttcgcg ttcagccggt ggttcaggtc ggcggtctcg agcggcgtcc
    16141 cgaggaacca gatgacgccg ccgacgccct cctgcgagac gaggcgggcg aggtccaggt
    16201 actccggatc gtcctcggcg cggtagacgc cgcgcgagcc gaccatcacg acctgggccg
    16261 ccttctcgcg cagcgtcagc gcggcgacgg cgggagcgtg tccgagggtg gggccggctc
    16321 ggaggccgtc cgagggaccg gcgccgggcc ggcccgacga gcaggccgag aacgccagga
    16381 gcagcgagag ggccgggagg aaggcgagcg ggccgccgga ggtccggcgg ccccccatga
    16441 gaggtctttc ggcgtgcgtc acggcggccg gatcataggc tcggccgccg tgacgcgggg
    16501 ctcacggcac gatcatcggc acgtagctcc cgtcgtcgtt cccctggtcg ttcaggacgc
    16561 cgtaggcgag gaactggtcg gtccccgcga ggcgccggat gcgcgcatag ccgtgaggga
    16621 cgccgtaacg cgccagcgcg gagtcgaact ggaggaactg gcccggcgcg agcgtgcagg
    16681 gggagcactg cggctccttc ccgagctcgt tgccgttcgg cccgaagtac gtcacctcga
    16741 gctggagcgg ctccacctgc ccgccgagcg catgcccgac ggcgaggttc gcgcgggtgc
    16801 ccccttcctg gccgcgcgtc cccgtctgct gcagcccgta gacccaggct tccgtgtcgg
    16861 cggcctcccc gatcggggtg tagccgtagg cgaggccgag gaggcctccg gcccgcgccg
    16921 tcgtcgtgcg gacgcccagg aagccgtcgc tcgtcgggat cggcgcctgc gtgtcgctct
    16981 ggtcctcccg gaactgcccg tcgaggaact gggcgaagac gctcgcgacg gtgcccgccg
    17041 gcgcggtgaa tccgatgcgt cgaagctcgg cgacgacgtt cgggacggtg aactgcgaac
    17101 gggccgggag gtcgaagtac ccccagtcgg gcaccgggtc tgtccgtggg acgagcgcga
    17161 agagtccgga gatgtcccgg ttcgtccggt tcgcgaacgt catctccgac gtgtagcccg
    17221 cggcctcgag gacgaccggg acgagggccg cggaggtgcg cgagggcgag tcgttcggga
    17281 ccatcgggac gaacgcgccg tcgccgttga ggttgtcgtt gaggacggcg tagcagacga
    17341 actggtcgga gccggacgtc cgccggacga ccgcgtagcc gtacctcagc ccgcgggcgg
    17401 cgagaggggt cgacttctgc tcccactgga agggggcgag gtcgaagagg tccttcgagg
    17461 cgtgcgcggc gcccgtgtcg ccgttgtagt aggtggcctc gaggcggacg gcgccccccg
    17521 cgcccccgcc ggcgttcacg caggcgaggt tcgagcgggt cccggcgctc ccggccgccg
    17581 aggtctgctg aaggccgaag acgaacgcct ccgtctgggc ggcgcggccg agcggcgtgg
    17641 cggggaaggc gaggccgaag cggccgatga cgcccttcgc gcggaggtcg gccgtcgccg
    17701 gcgtcgtcac ccggacctgc gcgccgaact ggacgaggtt gtcggcgccg gccacctcga
    17761 tccgcaggga ggcggtcttg ttcccggccg ggacgcccat gccggtggcg ctctggagga
    17821 acgcgaagac gtccggatgg acctgctgcc ccggcgggag agagtaggtc gacgaggact
    17881 cgaaggaacc ctttgcccgg aacgtcaggt tcagcgtccg ggaggaacgg ttcgtcagcg
    17941 tcagctcgga ggtgaagaag gccccgccgg cgccgctcga cgtcaggacg atcgggagga
    18001 ggtaggtgta gacgctcccg gtgccggtcg agccgtacga cacggtgacg cccttcgaga
    18061 ccgaacggct gccggcggcg ttggccgcgg tcagggtgac ggagtaggtg ccggggcttc
    18121 cccagacctt cgtcgggttc tgagccgtgg aggtggtgcc atccccgaag gaccagctcc
    18181 aggtggcggc tcctccctgc gtccggtcgg agaaggagac cgactggccg ggcttgggct
    18241 gcgcgggggt gaacgagaag tcgggcgtgg ggggcgaggt cggagacggc gtgccgccgg
    18301 cggggttgat ctcccacatc ccgcggcccc acgtcgccgc gcggagccgg cgcccgtcgg
    18361 gcgagaagag gaggtaggag acccaggtcg cggggaaatc gtcgccgaac cggccccacg
    18421 actggccctt gttcgtcgag cggaagatcc cgtacttcgt ccccgcgaac atcaggctcg
    18481 ggtcggtcgg ggagaagacg aggctgtcga ccggcgcgaa gaacgtgaac gagccgcccg
    18541 cgtaggtgat cgggacgccg atccccgagc tggccgggct ggcggtctgc tgctgctggt
    18601 tcaggccggc gatccgccag atgacgtgcc ggccctgccc cgcgacgatc tccgtcgcgc
    18661 tctcgagccc gacgtagaag gcgttcgggt cggcggggtc ccacgcgatc gcgttcacct
    18721 ggctcccgat gttgacgagc gatccgttct ggaagccgtt cagggtgtag acgacgtagc
    18781 cggcggccgt cccgaaggcg acgcggttcc ggtccgtcgg gcggatcgag atggaagagc
    18841 agctcgccca gctcttctgc acgttcgggc agggctgcgc ggagacgccg tcggagcaga
    18901 agtactcggc gaagaccggc tgccagtcgg acggggtcgc ggcgccgttg gaggtccggt
    18961 agatccgatc ggtcatcgtg tagaggaccc gcgggtcgac gggatcgatc gcgagccggg
    19021 agcggaacga cgtgaccggc gggacggagc actgatcctc ggtctgcgcg tcggcgggac
    19081 aggtcgtcag ccggaacgac tcctcgctct cgcctccgtc gtcggcgcgg gaggtgtagc
    19141 cgttgaagtt cgtcgcgtag acgatgctcg ggttctgggg attcacggcg cagtcgtagc
    19201 cgtcgccgta gaagatccgc ttccaggcgg tcgtgctgtc gccggagagg cgcatggtgc
    19261 cgttgtcctg cgtcccggcg tacaggcggt acgggtcggt cgggtgctgg gcgagggagg
    19321 agaacatccg cgcgacgacg ccgtcgttct tccaggcgaa ggagcggccg ccgttcgtcg
    19381 accggaagag gccgccgtcg gtccccatcc agaagctcga tcggcccggc tcgaacgcga
    19441 tgacgttgac gtccgcgtgg cagaactgcg ggtggcgctc ggggagcgtc cactgcgaga
    19501 tctgggtcca ggagaggccg ccgtcctccg acctccaggt gtcgaggccg ccggcgatga
    19561 ggcggtcggg gttcgtcggg tcgacggcga ggccgaggaa ggtccatccc tgggtcccgg
    19621 cgatgagggg atagccctgt ccggcgggcg gcccgagggc ggtcccccag ttctgcccgc
    19681 cgttgtcgct ccggaagacg ccgacctgcg agtagccgcg ctggaggtcg tagccggcga
    19741 cgagccagta gatccggttc gggttcgagg gggagatcgc gacgagggag cggatcggga
    19801 ttccgttgaa gccagcctgc tgggcgatgt cctcgaacgc gacctgccag gtgtccccgc
    19861 cgtccgtcga gaaggcgagg ccgccccaga gcccggccca gaggacgttc gggttcgccg
    19921 ggctgcgggc gatgttcacg ccctgccggc gccattcgag cccttcgccc gtcgcgggaa
    19981 gccggttcac ccaggtccgg ccgccgtcgg tcgagcggaa gatcccgttc gccgtcccga
    20041 ggacgacgtc ctcgggattc gacgggtgga cgtcgaggtc gtaggagtag agagaaggga
    20101 tgacgtcgaa cgcgcctccg ctcacggagg tcgtgttgaa gctccaggtc gagccgccgt
    20161 cattggagcg gagcgtcccg aggccgatga cctgcgagcc gctcagcggc gactgctctc
    20221 ccgtcgccgc gtagacgacg ttcgggttcg acggggcgac cgcgacggcg ccgaacgtca
    20281 ggagcggcag gtcgtcggtc aggttgaccg ggttcgcgta gtcggccccc gggccgccct
    20341 gccgcgtcag gagccagatc cctccgttcg cagtcgcgac gtagagcttc gacccgtcgc
    20401 gcgtgaaggc gtagtcgcgg acgcgtcccg cgtccgtcgt cgggccgagc gacttccagc
    20461 cggccgcggc gagcgtcgcg ttcagaggct gcggcccccc gtcgggcgtc cggaatcgga
    20521 gccgctcgcg atacagctcc tgggccaccg accggaggat cggcgccgcg gggatctcgg
    20581 cgcgctcgag gcgctcccag tcgcgccgcg cctcccagta gcgtcgccct tcgtgggtcg
    20641 cctctttctc gtgcgcccgc tcggtccggg cgccgaggag gacgaggttc gggaggttcc
    20701 gctttccgga gaacgggatc ggcccgtcga gcgcgggcgg agctgacggc gccgacgccg
    20761 tggccgtggc cgcggcgagg agcagcgcac cggagaggag cgcgaagggg cggagacgca
    20821 gcatggagac ctcccgcatc ggggccgctc ggcgcagccc cgttccgatc gaacggcttc
    20881 gagtttggcg ggatgctagc ggagacgggg ccggaagagg agtccgagct gcatcggtcg
    20941 atgacggacg tcacccttgc cgccgggcgc tccccgggcg gcggaccggc gctcgatcag
    21001 agggggggcc cgacttctgg ctcgctccgg tggagctcct cccaggtgcc ccgcttccgg
    21061 atcacccgga ccgcgtcgcc gtcgacgagg acctccgcgg ggagcgggcg ggcgttgtac
    21121 tcgctcgcca tgacgaatcc gtaggcgccg gcgtcgagga aggcgacgag gtccccggcc
    21181 gaaggccggg ggaggaggcg accctcggcg aggatgtcgc ccgtctcgca gacgtcgccg
    21241 gccacgtcga ggagcatcgg ctccccgtcg cgccccacga tccggacccg gtgcgccgcc
    21301 ccgtagagcg ccgggcgcat cagcgtgttg aagccggcgt cgacgttgac gaacgtcttc
    21361 accggcgtcg tcttcacgga gttgacgcgc gcgaccatca gccccgcctg cgcgacgagg
    21421 tagcgccccg gctcgatcca gagctcgggc tcgtatccca ggagctcgac ctcctcgtcc
    21481 cagatcggct tcagggcgcg cgcgaggtcc tcgggcgtca tgaccgcctg cccgtcggcg
    21541 aaggggatgc cgaggccgcc gccgaggtcg acgaaggaga gcgtcaggcc gagctcctcc
    21601 ttcagctcgc gcacgaacgc gagcatccgg cgggcggtct tctcgtagcc ggcggtctcg
    21661 gtgatctgcg agccgatgtg acagtgcacg ccggtgatcg cgagggcggg gagcgcgcgg
    21721 gcgagcgcgt acgcggcgcg ggcgtggccg ccgtcgaggt ggactccgaa cttcgactcc
    21781 gggatcccgg tgttgatctg gtggagcgtg tcggggtcga cgcccgggtt gatccggaag
    21841 gagatccgcg cggtcgtccc gaggcggagc gcggcggcgg agacctgcgc gagctcgtcg
    21901 aggttgtcga cgttgagaag gacgtcgtgc cggagcgcga aatcgatctc cgaggggctc
    21961 ttggaggacg aggtgaagag gacgtcgcgc ccggcggcgc cggcacgcag cgccgcgcgc
    22021 acctcgccct cggaaacgac ctcgggggcg agcccttcgg cgaggacccg cttcacgatc
    22081 tcgacgttcg tgttcgcctt gagcgcgtag cggagcccga gccgcgggaa cctcggctcg
    22141 aacgccgcgc gcagggcgcg cgccttctcg cggatcgtct cggcgtcgta cacgtagagc
    22201 ggcgtgccgt ggtcggcggc gaggcgggtg agggcgtcga cggcctcggg gtgggccgct
    22261 tctccggcac ggacgcggag gaggccttcg cgcagcgtca tcgggggact ccccgcgcgc
    22321 cggggactcc ccgggcgccg gtctccgggc cgggcgggag aagggcggat gcggtctctc
    22381 tcatcgcgtc gggcgattct gcggcccgac gaccccgcgg tccagatgcc gcggcctaga
    22441 atcgcgtcgt gagaacgcgc cgcagcctgc tgtccgaggc cgccctcgcg tcggccttcc
    22501 tggcctttcc ccgctccgcc cgcgcggcgg ccccgcccgt tgcgcccgcg cccctgcggc
    22561 cgaaggcgct cgcgccggga tcgcgggtcg ctctcgtctc gccggcgagc ccgggtgcgg
    22621 aggcggagtc cgccgtgatc gccgaggagg tcgtcgcctc gctcggcctc gtcccgagga
    22681 ggatgccggc cgccgcccgg cagaccatgt acctcgccgg gacggacgag gagcgggcgg
    22741 ccgacctgaa cgccgcgttc cgcgacccgt cgatcgacgc ggtctggtgc atccgcggcg
    22801 gctatggctc cgggcggctg ctgccgctcc tggactggga ggcgatccgc aggaacccga
    22861 agccgctcct cggcttcagc gacatcaccg cgctcctgaa cggcttctac gcgaggacgg
    22921 ggctcgtcac gttccacgcg ccgacctgct cggagaacct ctccgactac acgctcgccg
    22981 agctgaagcg cgtcgtcttc acgcccgagg cggcgggcgt cgtcgccgcc gctccgcccg
    23041 tcgagccgaa agaaggattc gtcgaccggg aggaccgcct gcgtcggatc gtcccgggca
    23101 cgggccgggg ccgcctggtg ggcgggaaca tcagcgtctt ctcgatgctc gtcgggacgc
    23161 cctgggagcc gcctctcgcc ggctcgatcc tcttcctcga ggaggtgggg gaggagccct
    23221 accggatcga tcgctggctg acccatttcg tcctgacggg gcgactcgcc aactgcgccg
    23281 ggatcgtctt cggcaagttc aaggactgcg gccccgggga cgaggggagc taccagggga
    23341 cgtggacctg gcaggaggtc gtcgccgacc gcctcggcaa gctcggcgtc ccggtcctcg
    23401 cggggctccg gttcgggcac gtcgccgaca agtcgacgtt ccccgtggga gtcctggcgg
    23461 agctggacgt cgccgccggg accctgtcgc tcctcgagcc cgcggtctca tgagggaggg
    23521 gcgccgggcg cgccgagcgg ggcgggccgc gtgcgctctc ctcctggctc tcttcgtggc
    23581 gcctcccatc gcggcggcgg agccgagccg ggcgccggcc gggctgccgc tcgtcctcga
    23641 cacgggctgg cgggcggccg acgccgaccc tcccgacggc atcgcgggaa tcgaccggct
    23701 cgacttccgt ccggccgacc cggtttcgga ccaggcccgg cgcggtgaag tccgctggta
    23761 ccgggtcctc gtcgacctct ctccgttcgt cgggcagccg ctcgcgttcg cggccgacgg
    23821 cctccgcgac gtcgacgagg cgtggttcga cggcgtcccg atcggcggtc
//
LOCUS       DAOTEZ010000009        23009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_1469_length_23009_cov_6.996863, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000009 DAOTEZ010000000
VERSION     DAOTEZ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 23009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23009
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1469_length_23009_cov_6.996863"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /gene="ftsZ"
                     /locus_tag="P5164_00960"
     CDS             <1..891
                     /gene="ftsZ"
                     /locus_tag="P5164_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRY42487.1"
                     /translation="TAALEDAEKIAEALAGSDMVFVTAGMGGGTGTGAAPVVAKLASE
                     AGALVVAIVSKPFSFEGQRKSRFAEAGIAELREYVDTLITIPNERLYAFVDRNITAWE
                     AFRIADDVLRQAVQGISDLITVPGYVNVDFADVKTVMENMGMALMGTGTATGEHRAVE
                     AAQKAISNPLLEEQSIQGARAILINVTGGEDLTLAEFNEACQIVQQAADDDANIIFGL
                     VQDGAMKDQVKVSVIATGFDAPVAAARRVTEKPVERTRLPLAEEPIPEDSGYGVNLVQ
                     TRSPKDDIFDIPTFLRRQMD"
     gene            1238..3415
                     /locus_tag="P5164_00965"
     CDS             1238..3415
                     /locus_tag="P5164_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42488.1"
                     /translation="MKRDRALPRRSLRASGALLFAWAAAAAHAGNVQPLLRFGDADVS
                     TSAAARVVFSPMDAAGGADVVCAPADACTIPPGRYSIRLDSPDFVALDRPTFVSDTDA
                     AGVPTPFALRVFPAARVELGPGRFPEGSVLTVLDPESGLSFKATVRSRETSVQVPARK
                     VVAAVFRGAAEVAELRRPVTLTAGQHLELPENPPLTRGRGQAVVSLRFPASGTRGGNG
                     QAVVRLQAGGGVRSPDVAVTADPWRWTGIWYDLPSGSAAVEVESKAWALAEAPAFSVP
                     DRAVGFARELKVVPRPRLSAQFEPNDRLPAGEAQVDLLDCRRLKGVSGPPRFSLCTSA
                     STRNGPVAGEFRFEGLDPVLHALRWKAGRFQDYVLVDLEAAESKDVKIPVRIHRVKGV
                     VTRRGRPVTNARISWLHQVTGIESETVSDEEGRYEALLAPSGWYLTFLDGPGFKRHAD
                     ALDVSEDREADFVVPSNETEVRVVDAATGLPVEGARVGRHLEGKGDLHVARAESVECD
                     EDGLARLPPFPAGRVTVLARARGYRPSEEKRFDVTKESATTTIELRIEKGDGTRLRIV
                     DAAGSPIQGAWAMLPSGIRSEGADAAGEAFLESALAAGAPLFVTTPAGALALARFVAE
                     DAPVRVGPPCPPFTVRFLTADGRPVASEQIAYSIDGVRVPQPMDYLARLAAGGDLSSR
                     IDGTLRVAGLPAAGVVALWPARKPDAVVTRPLPVTELLELPAP"
     gene            3473..4105
                     /locus_tag="P5164_00970"
     CDS             3473..4105
                     /locus_tag="P5164_00970"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY42489.1"
                     /translation="MHGVSSRADPLDRTVTRFVELVTARIAPRIFRRQVHWHPSPPLI
                     QGGGTLVRQRDQLAETQSQRRRPRRLCDSPTRSLPDPRFRHPGYDELRGVVRAVSSDA
                     GLLAEDDALALVAHGLREVALRKAVSERTIRRRFDAAGTPVHVVVHRVRIEVTRSALV
                     ASVPTTVIARWLGFTNPEAYRRFVRREMGVSIREMRARVRESAIFVVARR"
     gene            4572..6824
                     /locus_tag="P5164_00975"
     CDS             4572..6824
                     /locus_tag="P5164_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRY42490.1"
                     /translation="MLVSILLSVLVGTEAGQAEQREEPPIRVEAIGKLADDALAAAFL
                     EFSDMNAKQRSTRMTVAQAEHGLLPPEHGDTSWAVTIQGKGLFGEGGLVWNREQRDLR
                     IRVWPEAFLVSDVSLSDQAVAPTLVRVRIELPAGSDERPYEEVIDCPVTKGKLRCAIP
                     CARMNLTLRVPGYASVRLWDVVPEPGREVDARALSFVPGPAVSGFVKALERNGPPCVG
                     CRVKVLLPRAGSDSSVEAGPGGTKTVGAYTDSRGYFQVRRLAPGVLPFRVDAAGYAPH
                     RDQVRLIGEQESELVEPIVLRRSSEVRIRIRPDLGPDGRPWQVQLLELRGDSYIKFAS
                     SMVTRDKPLVAQCATGVEYALEVKSAAGATWLFEPEVKFGQGVTERTVDLRIISIEGQ
                     VTIGDRPLRAEIRLAARDGGASVPFRSDDEGRFVASLPPGKDWIAQVKSESPKVVRTV
                     PLETCCSDGSFVEIVIPGDGRVSGRIVDERNEPVPEAIVRFVRQERDGEAQSLEVHGG
                     RFELHGLAAGQYYLSAEGSRSGSQQVLQSREVTVLVREADEEATDPVTLVLKDSRSFL
                     GRVVARSTGASLSGVRVERSFPSLLTGSVPAVTTSDSEGRFEVEIPVDAPRACLFFSG
                     PGVPSQLIGLSGTESGREIPLDDVGGLLVVEIVETRQPPSRPTWPLLVRDDCRIMPLQ
                     LGRGTERPAESGGVARVHTALVADGQWTLCSFDQKGLLGWPTVKPGPGSCLTGVVSAG
                     QRLELSRVID"
     gene            complement(7241..8209)
                     /locus_tag="P5164_00980"
     CDS             complement(7241..8209)
                     /locus_tag="P5164_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42491.1"
                     /translation="MTKRLLALAILSLAVSPVRAGSPAALLRSTDPAQVFARGRVSSE
                     FSEVKLALSPDGTRILWGSTNRPGGPGGWDLWESRKEGAAWSAPSAAPFCSPQNDFDP
                     SFAPDGSGVYFFSNRPGGSGGDDLWFAPLDSSSGRYGSARNLGPRVNSPGDEWAPVVS
                     PDGSTLLFATDGRPGAGLHDLFVSRLVDGVWREPLPLPAVNGPGEDFDAAYLDDGRSL
                     VFTRRTKDQEGADLWLAAWHDGRYAPPRRLGPEVNTEKGWNLGPSTTAAEPGVLYFSS
                     HRPDDTAGRLDIYRIGYRLVTAPADATDLPDCAPRPASRKKGANRP"
     gene            complement(8257..8571)
                     /locus_tag="P5164_00985"
     CDS             complement(8257..8571)
                     /locus_tag="P5164_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42492.1"
                     /translation="MIEAPANLRGLVKRRHRLVRLALLTLGWTLLVGGLVGGLIPFVQ
                     GWPFGVAGALILYVESRWFQRKVRRWRQRHPRVETVWLKLKAWRRSRRKARDGATPTA
                     EP"
     gene            complement(8568..9383)
                     /locus_tag="P5164_00990"
     CDS             complement(8568..9383)
                     /locus_tag="P5164_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRY42493.1"
                     /translation="MNEILPPGALEVLRTLCDGAPLVVFDLETTGPDRLTDRIVEVAA
                     VKVAPDGEVSVFETRVNPGVKIPSEATAVHGITDADVADCPTLAEVGPRLAAFLEGCD
                     LAGYNLRGFDIPLLGRELERVKVPFSFEGRRVVDAQVIYFRKEPRDLGAAVRAFVGRD
                     HAGAHSALADSIAAAEVLAGQLTRYDDLPRDIAALAAFTAPLEGRWVDADKRFVWRDG
                     AAVFNFGAKRGQPLADVAAKNPEYLDWMLEADFPDEAKRIVREAREGRFPTRR"
     gene            complement(9380..10111)
                     /locus_tag="P5164_00995"
     CDS             complement(9380..10111)
                     /locus_tag="P5164_00995"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HRY42494.1"
                     /translation="MTLRPLPVPAGAEAYLAFDKEAGQAAHGPRSLLAEAREALGDDL
                     HLVHRLDRITSGLLLLARGEEALRAAHAAWPTRVTKSYLALARGVPETAEGVIDLPLL
                     EHRTGKPWLLGRAVRAAYGPDRAARLLRGEGLSGIPPLPPPARCAAHPAGRPALTRWR
                     LLERRGDDALLELTPETGRMHQLRVHLAAIGHALRNDPLYDPACDPSGPPPLLRAVRI
                     VWAGPPGDPDGAFTFEAPASEMIPA"
     gene            complement(10108..10386)
                     /locus_tag="P5164_01000"
     CDS             complement(10108..10386)
                     /locus_tag="P5164_01000"
                     /inference="COORDINATES: protein motif:HMM:NF016109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HRY42495.1"
                     /translation="METFDSVLKVLVGAVGVGSGAAYVPQAVRIWKRRSSDDVSLLTY
                     LLFLLGQVIWLTYGIRFGLVEIILGMSANLAGSLAVVLSALRFRSRPA"
     gene            10430..10792
                     /locus_tag="P5164_01005"
     CDS             10430..10792
                     /locus_tag="P5164_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42496.1"
                     /translation="MRAGPLLAAFLLAGLTASAPGAELYGRPLRGLTPVPIAQLQRKA
                     SEYDGRTVRVQGEVRSERGTFRLVEGDASLRVTMRDADSALPSDANGASAAAEGVFRA
                     NGPGGAPGLEATGLELTR"
     gene            10861..12357
                     /locus_tag="P5164_01010"
     CDS             10861..12357
                     /locus_tag="P5164_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRY42497.1"
                     /translation="MRLGVDFGTTHTVVTMVDRGNYPVVGFEGGDPLPSLAAVLPSGE
                     LRFGFEAAAVRHEPGVSVLRSFKRLLADAGPRTGIEAGGRFVLLLDLLAGFLSQVRED
                     LLARSNAGIGEDEPLEAAVSVPANSSSAQRFLTLEAFRAAGWVPVALLNEPSAAGFEY
                     AHRFRSTITSRREHVLVYDLGGGTFDASLVRMTGRTNEVVTSEGVRRLGGDDFDEAIL
                     RAVLERAGAGEPDEETRDLLLEECARQKEAVGPNTRKLVLDLSPLDLPPVALPIEEAY
                     EACAPLVERTIEASEPVLAAAGVEESELAGVYVVGGAGAFPLVGRRLRERFGERRVKR
                     SPHPFAATAVGLALFLDGEAGYTLADRLTRHFGVFREAAAGEEVVFDPIFPKDLALPS
                     PGEAPLVAVRRYRAAHDLGHFRFVECSRIVDGRPDGDVAPWQDLKFPFSAALRGRRLD
                     GAAVSRLPEPGPEVEEVYSCSDAGAVSVTIRVPADGFSRTFRLGRPAL"
     gene            12467..13186
                     /locus_tag="P5164_01015"
     CDS             12467..13186
                     /locus_tag="P5164_01015"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HRY42498.1"
                     /translation="MNMRNPRLPAAAGAVRLVFGILIIVAGLLFTLDNFGFVSARFLI
                     RLWPVALIAVGLTKALQQKGFWNGFWGWVISLAGLLLLLDNLHVLRFPVWKLLPLVLV
                     ALGIRLVQKAGGRTFPDRTIESRTAATLDETAIFGGWDRAISTNEFLGGEINALFGGF
                     ELDLSGCRIPDDEAAVTVFIAFGGGDIRIPRDWNVEVRAFAIFGGTDDKSHHPPLDGD
                     VPPKKLVIDGFVVFGGLDIKN"
     gene            13188..14255
                     /locus_tag="P5164_01020"
     CDS             13188..14255
                     /locus_tag="P5164_01020"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRY42499.1"
                     /translation="MHPILARRERLFAYVLAWVPLAGIQAYLLALPGSLSWGEAAALS
                     GPLALVYAFLCLSSFYACRSAPLTRARTASVLGVHVVGALIAASLLTLAGSTLASLLG
                     SFPAFSTLPERFGQAVPPLFVLGVLLYLLSVTLHYILLSLESSQAAERRAMEARVLAR
                     EAELEALKAQLNPHFLFNALNSVSALTTSAPAKAREMCVLLSDFLRRSLSSGERTLIP
                     LREEIVLARQFLAVEQVRYGRRLEVEEEVEDSLLGVPVPPLLLQPLVENAVRHGVATL
                     IEGGTVTLRVEKAGDGVRIAVENPYDPETPAPAGSGLGLSLVRRRLAAVWGRAAELEE
                     GGDGKRFRVAVVVPPGRPEEA"
     gene            14258..14989
                     /locus_tag="P5164_01025"
     CDS             14258..14989
                     /locus_tag="P5164_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY42500.1"
                     /translation="MRVAVVDDEELARGLVLEHLAAHPDVTVVAECANGLEAIEAVAA
                     HAPDLVFLDVQMPRLTGFETLELLEPRPAVVFVTAYDRWALKAFEVHAVDYLLKPFSR
                     ERFDAALARARALLGAGARGPDPSLLAAAARPEGQALERVAVREGTRVTVLPVEAIDW
                     VGAEGDYVRIRSGDRSVLKLQSLADLAARLPADRFVRVHRSWVVNVARLAALEEGRTA
                     VMADGTRVPVSRAGAARLKELLPRG"
     gene            complement(15019..17013)
                     /locus_tag="P5164_01030"
     CDS             complement(15019..17013)
                     /locus_tag="P5164_01030"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRY42501.1"
                     /translation="MTLTRTFRVSGLAFFAGVLLTVLGGDAGIAEAMRVAGVLLLVFA
                     TPVFGVSLFRHLLRGLLWRVGSRLFVSYLLIGVLPLLLVAGLAAAALFILAGQSGARR
                     AEARLIARLDALEAKASEFASREKARRPELAGGSGAFDGWALLPASGAPEGDGPLGTA
                     LEKVSVPEGGLRALLRRGDRSFLAVGRRTKGGTLFLYRTADAALEDELSREAGLNVRF
                     GLGKTEAADGTRVAQAEGRRGSLTIDSPGQRTRVASVNREERKAQGARPPGPGDDGWV
                     VWFLALDLPVLDAGTGARVKDEGGALLLRTSFPAEFRALFGQAKLSDSGAASGTIALA
                     LIKGLATATLVVYLVAFGVAALLVARIARAARRLSHGFAQIEKGNFAVRERLRGRDQL
                     AELVSGFNEMAGHLEASVAARAEAEALGRELALARDLQRRLLPAPDFAFPGVELAADF
                     RPAAAIGGDFYHFLGEGETALTVVIADVAGHGLPAGIVMASALASLTALSRGGTDTPT
                     LLGLLDDEVRRTTERRAFVTLAHVRFDLEGRRAEFTNAGHVYPVRVAPRGSVSWVENP
                     AKPLGAGRPVERVTVTVPLVDDEVWVFLSDGVVEAMSPDGSEAFGFDRLEATLAACGG
                     KSAREVLEAVLAAWRAHTGGDEPQDDRTVVVVKVLARAAA"
     gene            17117..18568
                     /gene="cls"
                     /locus_tag="P5164_01035"
     CDS             17117..18568
                     /gene="cls"
                     /locus_tag="P5164_01035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HRY42502.1"
                     /translation="MSLTQILALVVVVADVLALVHAVTRHLGVTSTLAWIFFILFLPG
                     IGPLAYFLLASPRIRTTRRRKRLAGEAVRTAIRRNLGHEPLPAEIDASSPLARSVLFL
                     GTRLTGLPPRAGNRVELLLENEGAFRSKAAAIRGATRSVWAEYYIVEDDATGRAFLAD
                     LAERALAGCDVRLLYDAVGSSDIPDEGLEALRAAGGKTASFLPLNPLRRRWSAHLRNH
                     RKLLVVDEEIAFTGGMNVGDEYARGSRLAGGSGGPLRNRPWRDAHLRVEGPAVFDLAT
                     VFAEDWSFATGDRLVPGRIPMPVAGGSVVAVLPSGPDQTANANAHVYFSALSGALERC
                     WLTTPYFVPDEPTARALANAAFRGIDVRVLVPAESDVPVARAAGQFFYGPLLRAGVRI
                     FEYEPAVLHAKTVVVDGSWALVGSANFDFRSFALNFELGAVVYDRAFAALLEERFTRD
                     LADSREVTLAAVERRGFAQRARIGLARLLAPLL"
     gene            18582..20510
                     /locus_tag="P5164_01040"
     CDS             18582..20510
                     /locus_tag="P5164_01040"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRY42503.1"
                     /translation="MRLRSVPLLALLLSGLPLAGADVPIHGIQGAGASSPLVGQSVTT
                     TGVVTALRTNGFYLQAPDAEADADPATSEGVLVFTSSTPPAAAAVGNRVRVSGSVVEY
                     RPSADPASPPLTEIGGSVSVTFLSAANPLPAPVLLTSADLSPAGPADRLERYEGMRVH
                     VPALLVVAPTGGSVDEANATGRSNGVLVGVLPGTPRPFREAGSDPLTPLPAGSPCCVP
                     RFDGNPERLRVDTDGLAGAAPLDVASGTTLPDVVGPLDYGFRTYTLLPESRPVATGSP
                     AARAVPAPSADELTVGSLNLQRFFDAEDDGEGTTLNAAAFSTRLAKASLVIRTLLRTP
                     DVLAVVEVEDLSTLRALAARLSADALAAGDPDPAYQAFLEAGNDPGGIDVGFLVASRV
                     SVLSVIQEGKASTYTDPTTGSAATRNDRPPLVLTARWAAPGGTDLVFTVVANHLRSLN
                     DSEADSTTGRRVRAKRQAQAEFLAALLARLQAEGGGAPLLAVGDFNAFEVSDGLVDVI
                     GTVRGAPAPADQVVVNRSVDLVDPDLVDLCAVDALVPPSERYSYVFDGNAQLLDHALA
                     SAATLPLVRGVAFARLGADFPEAMRGDAARPERLTDHDGLVVRLGPAAPKGRSPVVPA
                     RDGEPPSPFRSRTPETAP"
     gene            20507..22177
                     /locus_tag="P5164_01045"
     CDS             20507..22177
                     /locus_tag="P5164_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42504.1"
                     /translation="MRSDLLARLEEARRLRPGARAAEEALEAAARFLPREAAHLARLH
                     DAALFLRAYPQSPRVLALAEALLARVPVHVTRLAGEGADLGPLDDPSAAGIAGVPVAM
                     PFSHAAASWLVRRDASALRVDWDAEDLPDRLAASLPRLVPFLEEQARVDANVPFREYV
                     DGARGARPDAAWLLERLAGLPLPDSVRAELWEALSLQLAWTPRASDSRGLARVPSPAP
                     FCSDGPLLSRRDVSVAAELAAAPPPLVRLSRSDGAALLDAARAAMAVRYRELPAFSAG
                     DPSAVFRCDVGRGVSIFVTGLDRSARLPLRAAFGTLFARNGVLVGYGDLHAALGRADV
                     SFNVFYAFRDGESAWLYARLLAVARAVTHAAQLSVDPYQIGLGNEEAIASGAFWFYRK
                     LGFRSADPGLERLARREEARSSASPGRRTPPATLRRLAAAPLLLDVPGETPFLPDSFR
                     LAGLGLAAARRFAASKLSPEAFRARAARRVSRALGLDPVPPALRAPLEALVPVLALLD
                     DLTSWPLSHRRAVAALLRAKAAPDELRYARLSSAHARLAASIARAVSS"
     gene            complement(22494..>23009)
                     /locus_tag="P5164_01050"
     CDS             complement(22494..>23009)
                     /locus_tag="P5164_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42505.1"
                     /translation="GALLSATKTGSGTFSAGATVTYAIVLRNDGIGPQADAAGPELLD
                     VLPTTLALSGATASAGTASADAGTRTVTWNGALAAGASVTITITATVLPTAAGTVVSN
                     QGALSWDANGDGTNDAAGSTDDPRLPGASDPTDFPVPVEAARDIPVLGPFGALLMAAL
                     VAAAGLRFRTR"
BASE COUNT         3015 a         8446 c         8273 g         3275 t
ORIGIN      
        1 accgccgccc tcgaggacgc cgagaagatc gccgaggcgc tcgccggctc cgacatggtc
       61 ttcgtgaccg cggggatggg cggcggcacc ggcaccggcg cggcgcccgt cgtcgccaag
      121 ctcgcctcgg aggcgggcgc cctcgtcgtc gcgatcgtct ccaagccgtt ctccttcgag
      181 gggcagcgca agagccgctt cgccgaggcg ggaatcgccg agctgcgcga atacgtcgac
      241 acgctgatca cgatcccgaa cgagaggctc tacgcgttcg tcgacaggaa catcacggcc
      301 tgggaggcgt tccgcatcgc cgacgacgtc ctccggcagg cggtgcaggg gatcagcgac
      361 ctgatcaccg tcccgggcta cgtgaacgtc gacttcgccg acgtgaagac cgtcatggag
      421 aacatgggga tggccctcat ggggaccggc accgcgacgg gcgagcaccg cgccgtcgag
      481 gcggcccaga aggcgatctc gaacccgctc ctcgaggagc agtcgatcca gggcgcccgc
      541 gcgatcctca tcaacgtcac gggcggcgag gacctcacgc tcgccgagtt caacgaggcg
      601 tgccagatcg tccagcaggc ggccgacgac gacgcgaaca tcatcttcgg cctcgtccag
      661 gacggcgcga tgaaggacca ggtgaaggtg agcgtcatcg cgaccggctt cgacgccccc
      721 gtggcggcgg cccgccgcgt cacggaaaag ccggtcgagc ggacccgtct ccccctcgcc
      781 gaggagccga tccccgagga ctccggctac ggagtgaacc tcgtccagac ccggagcccg
      841 aaggacgaca tcttcgacat cccgacgttc ctccggaggc agatggactg aggccgggac
      901 ccgccccgcg ccccccgccc ttcgcggtgc gcgcttcgcg cggtctgaga tgaagacggg
      961 gcgcggaggg agatagatgg gggggcgcgg tcgtcctcgt ggtgctttcg ctcgtcgcga
     1021 cggccaagct ctcgagcgca gacacgttct gcggcttcta ctgtgccggc accttgggta
     1081 acagcatctg tcagggaccc gcgcagccga cgatggggtg cacctatgtc ggcggactcg
     1141 gaagctgcat gagcatctcg gcaccacagt gctcgggcga cccagactgc ggcttcgacg
     1201 gtgagctctg tccccggggc cccttcaacc agctgccttg aaacgagacc gcgcgcttcc
     1261 tcgccgttcc ctgcgggcgt cgggggccct cctcttcgcg tgggcggccg cggccgccca
     1321 cgcgggcaac gtccagccgc tgcttcgttt cggcgacgcc gacgtctcca cgtcggccgc
     1381 cgcccgcgtc gtcttctctc cgatggacgc ggccggcggc gcggacgtcg tctgcgcgcc
     1441 ggccgacgct tgcacgatcc ctccgggccg ctactcgatc cggctcgact cgcccgactt
     1501 cgtcgcgctc gaccgtccga cgttcgtctc cgataccgac gctgcgggcg tcccgactcc
     1561 cttcgcgctc cgggtctttc cggcggcccg cgtggagctc ggcccgggcc gtttcccgga
     1621 ggggagcgtc ctgaccgtcc tcgatcccga gtccggcctc agcttcaagg ccaccgtccg
     1681 ctctcgggag acgtccgtgc aggttcccgc gcggaaggtc gtggcggcgg tctttcgcgg
     1741 cgcggccgag gtggccgagc tgcggcggcc cgtcaccctt accgcggggc agcacctcga
     1801 gcttccggag aacccgcccc tcacgagagg gagggggcaa gccgtcgtga gcctccgctt
     1861 cccggcctcc ggcacgcgcg ggggcaacgg gcaggccgtg gtgcgcctcc aggcgggagg
     1921 aggggtgcga tcgcccgacg tggccgtcac cgcggacccc tggagatgga ccgggatctg
     1981 gtatgacctt ccctccgggt ccgccgcggt ggaggtggag tcgaaggcct gggccctcgc
     2041 cgaggctccc gcattcagcg tcccggacag ggccgttggc ttcgctcggg agctgaaggt
     2101 cgtccctcgt ccccgcctga gcgcgcaatt cgagccgaac gaccgactcc ccgccggcga
     2161 ggcgcaggtc gacctgctcg actgccgccg cttgaagggc gtgagcggcc cgccccgctt
     2221 ctcgctctgc acgtcggctt cgacccggaa tgggcccgtc gcgggcgagt tccgtttcga
     2281 gggcctcgac cccgtgctgc acgcgcttcg ctggaaggca ggccggttcc aggactacgt
     2341 cctcgtcgat ctcgaagcgg ccgagtccaa agacgtgaag atccccgtca ggattcaccg
     2401 cgtgaagggg gtggtcaccc ggcggggtcg tccggtgacc aacgcccgga tctcctggct
     2461 gcatcaggtg accggcatcg aatccgagac cgtttcggac gaggagggcc gctacgaggc
     2521 cctcctcgct ccttccggct ggtacctcac gttcctcgac ggcccgggct tcaagcgaca
     2581 cgcggacgcg ctggacgtgt cggaagaccg cgaagcggac ttcgtcgtcc cttcgaacga
     2641 gaccgaggtc cgggtcgtcg acgcggcgac cggcctgccc gtcgagggcg ctcgtgtcgg
     2701 aaggcacctc gagggaaagg gcgacctgca cgtcgcgcgc gccgagagcg tggagtgtga
     2761 cgaggacggg ctggcccggc tccccccgtt cccggccggg cgcgtcaccg ttctggcgcg
     2821 ggcgcgcgga tatcggcctt ccgaggagaa acggttcgac gtcacgaagg agagcgccac
     2881 gacgacgatc gagctccgga tcgagaaggg cgacggaacg cggctcagga tcgtcgatgc
     2941 cgccggcagc ccgatccagg gagcctgggc gatgctcccc tccggaatcc gctcggaggg
     3001 cgcggacgcc gcgggcgagg cgttcctcga atcggcgctc gcggccgggg cgccgctctt
     3061 cgtcacgacg ccggccggcg ctctcgctct ggcccggttc gtggccgagg acgctcccgt
     3121 acgggtcggg ccgccttgtc cccccttcac cgtccggttc ctgacggcgg acgggcgccc
     3181 ggtggcttcc gagcagatcg cctactcgat cgacggcgtt cgagtccccc agccgatgga
     3241 ctaccttgcc cgactcgccg ccggaggcga cctcagctcc cggatcgacg gaacgctcag
     3301 agtggccggc ctccccgccg ccggcgtcgt cgccctctgg ccggcccgaa agcccgacgc
     3361 cgtcgtcaca cgcccgctgc ccgtcaccga gctcctcgag ctcccggcgc cctgagcgaa
     3421 atcgaggcgc ggccctgctg cagccgttct cgcgagtgac gccccagagc ggatgcacgg
     3481 cgtctcctct cgggccgacc ctctcgaccg gacggtgacg cggttcgtcg aactcgtgac
     3541 agcaagaatc gcgccaagaa tatttcgtcg gcaggtgcac tggcacccct ctccacccct
     3601 catacaaggt ggagggactc tcgtgcgtca acgagatcaa ctcgctgaaa cacagtcgca
     3661 gcgtcgccgg cctcgtcgtc tgtgcgacag cccgacccgc tcccttcccg acccgaggtt
     3721 ccggcatccc ggatacgacg agctccgcgg ggtcgtccgt gcggtgagct ccgacgcggg
     3781 cctcctcgcg gaggacgacg cgctcgctct cgtcgcccat ggacttcgag aggtcgcgct
     3841 gcgaaaggcg gtctcggagc ggacgatccg acgcaggttc gacgcggccg gaacgccggt
     3901 ccacgtcgtc gtccatcgcg tccggataga ggtgactcgg tccgcgctgg tcgcctccgt
     3961 cccgaccacg gtgatcgcgc gctggctcgg cttcacgaac ccggaggcat accgaaggtt
     4021 cgtgaggcgc gagatgggcg tgtcgatccg ggagatgagg gctcgtgtcc gcgaaagtgc
     4081 gatattcgtc gtcgcgcggc gttgaaacaa tcggtcgccc atgcctaaac tcctcgaaac
     4141 aaaagaatgc gcaagagcgc cggccccctc ccggagcatc gaaggagacc ctgagtgaga
     4201 aagctgttct ttggaccctc cgcgctttcc atcgtcatcg cgctggttat tgccacgagt
     4261 gccgtcgcgc aggagggccc acggcccccg agggaagccg agcatcccga tggggggttc
     4321 gaaggatggg cggaaccagg tgactgggat tggtatgttg ccggtggcgg tggaaaaccc
     4381 ggcacttgca cgaaatacgc atgcaaggac tgcctgccaa atatctactt gcaggtagac
     4441 gagtgcgtcc tcgtctcgag agctaaggca tgtgactgtg atacagttct gccgaagggc
     4501 gcgacgaaga cagtttgccg actcaagggc gtttgcacct gggtgatccg cgtctgaggg
     4561 gggcaaacac cgtgctcgtg tcgatcctgc tgagcgtctt ggtcgggacc gaagccggac
     4621 aggccgagca gcgagaggaa ccgccgatcc gcgtcgaagc catcggaaag ctggccgacg
     4681 atgcgctcgc agctgcattc ctcgaattct cggacatgaa cgcgaagcag cgatcgactc
     4741 gcatgaccgt ggctcaggcg gaacatggcc ttcttccacc ggagcacggg gatacctcat
     4801 gggccgtgac catccaggga aagggactct tcggagaggg tggactcgtt tggaatcgcg
     4861 agcagcggga cttgcgaata agggtctggc cagaggcatt cctcgtatcg gacgtgtcgc
     4921 tgtcagacca agcggtcgct ccgaccctgg ttcgtgtacg aatcgagctc ccggccggat
     4981 ccgacgagcg accgtatgag gaggtcatcg attgcccggt gaccaaaggc aaactgcgct
     5041 gtgccattcc gtgtgcgcgg atgaacctga cgctgcgcgt cccaggctat gcgagcgttc
     5101 ggctgtggga cgtcgtgcct gagccgggcc gggaggtcga cgcgagagcc ctttcattcg
     5161 tgcccggccc agccgtgtcg ggtttcgtga aggcgctgga gcgaaatggt ccgccctgcg
     5221 tcggttgccg cgtgaaggtt ctattgccca gggcgggctc cgattcaagc gtcgaagccg
     5281 gaccgggtgg aacgaagacg gtcggtgcgt acacggactc acgggggtac ttccaggtga
     5341 ggcgactagc gcccggcgtg ctgcctttca gagtcgatgc cgcgggatac gcgccgcaca
     5401 gggaccaagt gcggctcatc ggcgagcagg aatcggaact cgttgagccg atcgtccttc
     5461 gtcgttcgag cgaggtgaga attagaatcc gaccggatct tggccccgac gggcggccgt
     5521 ggcaggtgca actgctggag cttcgaggag acagctatat caagttcgcg tcctcaatgg
     5581 tgacgcgaga caagcctcta gtcgcacagt gtgcgaccgg cgtcgagtac gcgctagagg
     5641 tgaagtcggc cgccggcgca acctggctct ttgagcccga agtgaagttt gggcaaggcg
     5701 tcaccgagcg aactgtcgac ctgcggatca tctccatcga ggggcaggtc actatcggag
     5761 accggccgct ccgggcagaa atacgcctgg cggcgaggga tgggggcgct tcggttccct
     5821 tccggtctga cgatgaaggg cgcttcgtcg ccagcctccc cccggggaag gactggatcg
     5881 cccaggtgaa gagcgaaagc cccaaggtcg tccgtacggt gccactcgag acttgctgca
     5941 gcgacgggtc cttcgtcgag atcgtgatcc caggcgatgg acgtgtatcg ggccgcatag
     6001 tcgatgagcg gaacgagccc gttccagagg cgatagtgag gttcgtccgc caagaacggg
     6061 atggcgaggc gcagagcctg gaggttcacg ggggacgctt tgagcttcac ggactagcgg
     6121 cgggccagta ctacctgtcg gcggagggta gccggtccgg cagtcagcaa gtccttcaga
     6181 gccgggaggt gaccgtgctg gtgagggagg ccgacgagga ggctaccgat ccggtgaccc
     6241 tcgtcctcaa ggactcgagg tcattcctgg gccgggtcgt ggcgcgaagc acgggagcat
     6301 ccctgtccgg agttcgcgtg gagagatctt tcccgagcct tctgactggc tccgtccccg
     6361 cggtgacgac aagcgactcc gaggggcgct tcgaggtgga gatcccagta gatgcgccga
     6421 gggcgtgtct cttcttctcg ggtccgggag tcccatcaca gttgatcgga ctgagcggaa
     6481 ccgaaagcgg gcgggaaatt ccgctggacg atgttggtgg cctactcgtc gtcgaaatcg
     6541 tggagacgcg gcagccgccc tctcggccga cctggcctct tctggtccgt gacgactgcc
     6601 gaatcatgcc cctgcaactc gggcggggca cggagcgccc cgcggaatcg ggcggcgtgg
     6661 cccgggtaca cacggcgctc gtggcggacg ggcaatggac cctctgctca ttcgaccaga
     6721 aagggctact cggatggccg accgtcaagc ccgggccggg ttcgtgcctc accggcgtcg
     6781 tctccgcagg ccagcggctc gagctttcga gagtgatcga ctagcaggcc gctgaaaaga
     6841 tcggtcttgg accgttgaca gaacggccac agcgggccac cgtgtcgacg tgcgcggaga
     6901 catcgaaaag cagcccgatc tgatcgtcac ggtgagcccg gaggacctgg ttcccccgga
     6961 tcaccggctt cgcagtcggg gccgtgtcta cactcgtgta ttgtgtgatc tgggcaggga
     7021 tgcaattccg ggcgcacctc ggtcttcttt cgggcgcttc ttagcatccc cacgatagcc
     7081 ggaatccggc cgagggtcgg gcgtgtccaa ggctctcgaa gcgacgcgcg atcgacgcgc
     7141 cgcttctcgc cccggccgcg atcgctctcg ggctcgtccc cctcgtcgcc ttcgccccgg
     7201 tgcgtgacgc ggggcgacct cccgaggcgc cgagcgccgc ctacggccgg ttcgcgccct
     7261 tcttccgtga cgcgggccgg ggtgcgcaat cggggaggtc ggtcgcgtcg gcgggcgccg
     7321 tcacgagccg atagccgatc cggtagatgt cgaggcgccc ggcggtgtcg tcgggtctgt
     7381 gcgaggagaa gtagaggacg ccgggctctg ccgcggtcgt ggagggaccg aggttccagc
     7441 ccttctctgt gttgacctcg gggccgagtc ggcgtggcgg ggcgtaacga ccgtcgtgcc
     7501 acgccgcgag ccagaggtcg gcgccctcct ggtccttcgt ccgtcgcgtg aagacgaggc
     7561 tgcgaccgtc gtcgagatac gcggcgtcga agtcctcgcc agggccgttc acggccggca
     7621 gcgggagggg ctcgcgccac accccgtcga cgagccgcga gacgaagagg tcgtgaaggc
     7681 ccgccccggg ccgaccgtcc gtcgcgaaga ggagcgtcga gccgtcgggg ctgacgacgg
     7741 gcgcccactc gtcgccgggc gagttcacgc gtgggccgag gttgcgcgcg gagccgtacc
     7801 ggccggaaga ggagtcgagc ggcgcgaacc agaggtcgtc cccgccggag cccccggggc
     7861 ggttgctgaa gaagtagacg ccgcttccgt cgggagcgaa cgaggggtcg aagtcgttct
     7921 gcggcgagca gaagggggcg gccgaaggag cgctccaggc ggcgccctcc ttgcggctct
     7981 cccagaggtc ccagccgcca gggccgccag gccggttcgt ggagccccag aggatgcggg
     8041 tgccgtcggg gctgagggcg agcttgacct cgctgaactc cgaggagacc cggccccgcg
     8101 cgaagacctg ggccgggtcg gtggagcgaa ggagcgcagc cggggagcca gcgcggacgg
     8161 gggagacggc gagggagagg attgcgaggg ccagtagccg cttcgtcacg gagggattct
     8221 cctcccgagc ggaccgcgca acgcgcgctg aaggacctac ggctcggccg tcggcgtcgc
     8281 gccgtctctc gccttccgcc ggctgcggcg ccaggccttg agcttcagcc agaccgtctc
     8341 gacgcgcggg tggcgctggc gccagcggcg gaccttgcgc tggaaccatc ggctctcgac
     8401 gtagaggatc agcgcgccgg cgactccgaa gggccacccc tggacgaacg ggatgaggcc
     8461 gccgacgagg ccgccgacga ggagcgtcca gccgagcgtg aggagcgcga ggcgcaccag
     8521 acggtggcgg cgcttcacga ggccgcggag gttcgcgggg gcctcgatca cctgcgcgtc
     8581 gggaagcgtc cctcgcgggc ctcgcggacg atccgcttcg cctcgtcggg gaagtcggcc
     8641 tcgagcatcc agtcgaggta ctcggggttc ttcgcggcga cgtcggcgag cggctggccg
     8701 cgcttggcgc cgaagttgaa gacggcggcg ccgtccctcc agacgaagcg cttgtcggcg
     8761 tcgacccagc gcccctcgag gggcgccgtg aaggcggcga gggcggcgat gtcgcgcggg
     8821 aggtcgtcgt agcgcgtcag ctggcccgcg aggacctccg cggcggcgat cgagtcggcg
     8881 agcgcggagt gggcgcccgc gtggtcccgc ccgacgaagg cgcggacggc ggccccgagg
     8941 tcgcgcggct ccttgcggaa gtagatgacc tgggcgtcga cgacgcggcg cccctcgaac
     9001 gagaacggca ccttgacccg ctcgagctcg cggccgagga gcgggatgtc gaatccgcga
     9061 aggttgtagc cggccaggtc gcacccctcg aggaacgccg cgaggcgcgg gccgacctcg
     9121 gcgagcgtgg ggcagtcggc gacgtcggcg tcggtgatcc cgtggacggc ggtcgcctcg
     9181 gaggggatct tcacgcccgg gttgacgcgc gtctcgaaga cggagacctc cccgtcgggc
     9241 gcgaccttca cggcggccac ctcgacgatc cggtcggtga ggcggtcggg gcccgtcgtc
     9301 tcgaggtcga agacgacgag cggggcgccg tcgcagagag ttcgcaggac ctcgagggcc
     9361 ccggggggga ggatctcgtt cacgccggga tcatctcaga cgcgggcgcc tcgaacgtga
     9421 aggcgccgtc cgggtcgccg ggagggcccg cccagacgat ccggacggcg cgcaggagcg
     9481 gcggcgggcc ggacgggtcg caggccgggt cgtagagcgg gtcgttgcgg agagcgtggc
     9541 cgatcgcggc gaggtggacg cgcagctggt gcatccgccc ggtctcgggc gtcagctcga
     9601 ggagggcgtc gtcgccgcgc cgctcgagga ggcgccagcg ggtgagggcg gggcgtccgg
     9661 cggggtgcgc ggcgcagcgg gcgggcggcg ggagcggcgg gatgccggag agcccctcgc
     9721 cgcgaaggag ccgcgcggct cggtcggggc cgtaggcggc gcggacggcg cgcccgagga
     9781 gccacggctt gccggtgcgg tgctcgagga gcgggaggtc gatcaccccc tcggccgtct
     9841 cggggacgcc gcgcgcgagc gcgaggtagc tcttcgtcac gcgcgtcggc caggccgcgt
     9901 gggccgcgcg aagcgcttcc tcgccccgcg cgaggaggag gaggccggag gtgatccggt
     9961 cgaggcggtg gacgaggtgg aggtcgtcgc cgagcgcctc gcgcgcctcg gcgaggaggc
    10021 tccgggggcc gtgcgcggcc tgccccgcct ccttgtcgaa cgcgaggtac gcctcggccc
    10081 cggccggcac cgggaggggg cgaagcgtca cgccggtcgg ctgcggaagc ggagcgccga
    10141 gaggacgacg gcgaggctcc cggcgaggtt cgccgacatc ccgaggatga tctcgacgag
    10201 gccgaagcgg atgccgtacg tcagccagat gacctggccg aggaggaaga ggaggtacgt
    10261 caggagcgac acgtcgtcgc tcgatctccg cttccagatc cggaccgcct gggggacgta
    10321 ggcggcgccg ctcccgacgc cgaccgcacc gacgaggacc ttgaggacgg aatcgaaggt
    10381 ctccatgggg cggggagcct agcagggcgg ccggccctac aatggaggca tgagagcggg
    10441 accgctcctc gccgcgttcc tcctcgccgg gctgacggcg tcggcgccgg gggcggagct
    10501 ctacggccgc ccgctgcgcg ggctgacgcc cgtcccgatc gcccagctcc agcggaaagc
    10561 ctccgaatac gacggccgga cggtccgcgt gcagggcgag gtccgctcgg agcgcgggac
    10621 gttccgcctg gtcgagggag acgcgtccct ccgggtgacg atgcgcgacg cggactcggc
    10681 cctcccgtcc gacgcgaacg gcgcgtcggc ggcggcggaa ggggtcttcc gcgcgaacgg
    10741 tccgggaggc gcgcccggcc tcgaggcgac gggcctcgag ctgacgcgct gaggcggctc
    10801 tcggctccgc gcgggaggcc gggccgcgcc ccttccgaaa gcccgtaaac tcgggacgcc
    10861 atgcgcctcg gcgtcgactt cggaacgacc cacacggtgg tgacgatggt cgaccgcggg
    10921 aactaccccg tggtcggctt cgaggggggc gacccgctcc cgtcgctcgc ggcggtcctc
    10981 ccgtcggggg agctccgctt cggcttcgag gcggcggccg tgaggcacga gccgggggtg
    11041 agcgtcctcc gctcgttcaa gaggctcctc gccgacgcgg gaccgcggac cgggatcgag
    11101 gccggcggcc gtttcgtcct cctcctcgac ctcctcgcag ggttcctctc gcaggtgcgg
    11161 gaggacctcc tcgcccgctc gaacgccggg atcggcgagg acgagccgct cgaggcggcg
    11221 gtctccgtcc ccgcgaactc ctcgagcgcc cagcgcttcc tgacgctcga ggcgttccgc
    11281 gcggccgggt gggtgcccgt cgccctcctg aacgagccgt cggccgcggg cttcgagtac
    11341 gcgcaccgct tccggtcgac gatcacctcg cgccgggagc acgtcctcgt ctacgacctc
    11401 ggcgggggga cgttcgacgc ctcgctcgtc aggatgacgg ggaggacgaa cgaggtcgtc
    11461 acgagcgaag gggtgcgccg cctcggcggc gacgacttcg acgaggcgat cctccgggcc
    11521 gtcctcgagc gggccggagc cggggagccg gacgaggaga cccgcgatct cctcctcgag
    11581 gagtgcgcgc ggcagaagga agcggtcggg ccgaacacga ggaagctcgt cctcgacctg
    11641 tcgccgctcg acctcccgcc ggtcgccctt ccgatcgagg aggcgtacga ggcctgcgcg
    11701 ccgctcgtcg agcggacgat cgaggcgagc gaaccggtcc tggccgcggc gggagtggag
    11761 gagtccgagc tcgccggcgt ctacgtcgtc ggcggggcgg gggcgttccc tctcgtgggg
    11821 cggcggctcc gggagcggtt cggagagcgg cgcgtgaagc gctcgcccca tccgttcgcg
    11881 gcgaccgccg tgggcctcgc cctcttcctc gacggcgagg cgggctacac gctcgccgac
    11941 cggctgacgc ggcacttcgg cgtcttccgc gaggcggcgg cgggggagga ggtcgtcttc
    12001 gacccgatct tcccgaagga cctcgccctt ccgtcgccgg gcgaggcgcc gctcgtggcc
    12061 gtccgccgct atcgggcggc gcacgacctc gggcacttcc gattcgtgga gtgcagccgg
    12121 atcgtcgacg gccgccccga cggggacgtc gcgccgtggc aggacctgaa gttcccgttc
    12181 tccgccgcgc ttcgcggccg gcggctcgac ggcgccgcgg tcagccggct ccccgagccg
    12241 ggcccggagg tggaggaggt ctactcctgc tccgacgccg gggcggtctc ggtgacgatc
    12301 cgcgtcccgg ccgacggctt ctcgcgcacc ttccggctgg gccggccggc gctctgagag
    12361 ccgttcgtcg acccccggcg cccgttcgcc gaatcgtccg ggcggaatcc ccgcggccga
    12421 cgtaaggtgg aggagacccc ccggagaggg ggcgcggagg caggccatga acatgagaaa
    12481 cccgagactt ccggccgcgg cgggcgcagt gaggctcgtc ttcggcatcc tgatcatcgt
    12541 cgcgggcctc ctcttcacgc tcgacaactt cggcttcgtg tcggcccgct tcctcatccg
    12601 cctctggcca gtcgcgctga tcgcggtcgg gctgacgaag gcgctccagc agaagggctt
    12661 ctggaacggc ttctggggat gggtgatctc cctcgcgggg ctcctgctcc tcctcgacaa
    12721 cctccacgtc ctccgcttcc cggtctggaa gctcctcccg ctcgtcctcg tggcgctcgg
    12781 gatccggctc gtccagaagg cgggcgggcg gactttcccc gaccggacga tcgagagccg
    12841 gacggccgcc acgctcgacg agacggcgat cttcggcggc tgggaccgcg cgatctcgac
    12901 gaacgagttc ctcggcgggg agatcaacgc cctcttcggc ggcttcgagc tcgacctgtc
    12961 gggctgccgg atccccgacg acgaggcggc cgtcaccgtc ttcatcgcgt tcggcggcgg
    13021 cgacatccgc atcccgcgcg actggaacgt cgaggtccgc gcgttcgcga tcttcggcgg
    13081 caccgacgac aagtcccatc acccgcccct cgacggagac gttccgccga agaagctcgt
    13141 catcgacggc ttcgtggtct tcggcggcct cgacatcaag aactgacgtg cacccgatcc
    13201 tggcccgccg ggaacggctc ttcgcctacg ttctcgcctg ggtccccctg gcggggatcc
    13261 aggcgtacct cctcgccctg cccgggagcc tctcctgggg cgaggcggcg gccctctcgg
    13321 gcccgctcgc gctcgtctac gccttcctct gcctctcgtc cttctacgcc tgccgcagcg
    13381 cgccgctcac ccgcgctcgg acggcctccg tcctgggtgt ccacgtcgtc ggcgcgctca
    13441 tcgccgcctc gctcctgacg ctcgccggct cgacgctggc gagcctgctc gggtcgttcc
    13501 ccgcgttctc gaccctcccc gagcgcttcg ggcaggcggt gccgccgctc ttcgtcctgg
    13561 gggtcctcct ctacctcctc tcggtcacgc tccactacat cctcctctct ctcgagtcgt
    13621 cccaggcggc ggagcgtcgg gcgatggagg cgcgggtcct ggcgcgcgag gcggagctcg
    13681 aggcgctcaa ggcccagctg aacccccact tcctcttcaa cgcgctcaac tccgtcagcg
    13741 ccctgacgac gagcgcgccg gcgaaggcgc gcgagatgtg cgtcctcctc tccgacttcc
    13801 tccggcggag cctctcctcc ggcgagcgga cgctcatccc gctccgggag gagatcgtcc
    13861 tggcgcgcca gttcctcgcg gtggagcagg tccgctacgg gcggaggctc gaggtggagg
    13921 aggaggtcga ggactccctc ctcggcgtcc ccgtcccccc gctcctcctc cagccgctcg
    13981 tcgagaacgc cgtccgccac ggcgtcgcga cgctcatcga aggggggacg gtgacgctcc
    14041 gcgtcgagaa ggcgggcgac ggcgtcagga tcgccgtcga gaacccgtac gacccggaga
    14101 ctccggcccc ggccgggagc ggtctcggcc tctcgctcgt tcgccgccgt ctcgccgccg
    14161 tctgggggcg cgcggccgag ctggaggagg ggggggacgg gaagcggttc cgcgtcgccg
    14221 tcgtcgtccc gcccgggagg ccggaggagg cgtgagcctg cgcgtcgcgg tcgtcgacga
    14281 cgaggagctg gcgcgggggc tcgtcctcga gcacctcgcg gcgcatcccg acgtgacggt
    14341 cgtcgccgag tgcgcgaacg gcctcgaggc gatcgaagcc gtcgccgcgc acgcgcccga
    14401 cctcgtcttc ctcgacgtcc agatgccgcg cctgaccggc ttcgagacgc tcgagctcct
    14461 cgagccgcgc ccggccgtcg tcttcgtcac cgcgtacgac cgctgggcgc tgaaggcgtt
    14521 cgaggtgcac gcggtcgact acctcctcaa gccgttctcg cgggagcggt tcgacgcggc
    14581 gctcgcgcgg gcccgggcgc tcctcggcgc cggcgcgcgc ggccccgacc cgtccctcct
    14641 cgcggcggcg gcccggcccg aagggcaggc tctcgagcgg gtcgcggtgc gcgaggggac
    14701 gcgcgtcacg gtcctcccgg tcgaggcgat cgactgggtc ggcgcggagg gggactacgt
    14761 gaggatccgc tccggcgacc ggagcgtcct caagctccag tcgctcgccg acctcgccgc
    14821 gcgcctcccg gccgaccgct tcgtccgcgt ccatcgctcc tgggtcgtga acgtcgcccg
    14881 cctcgccgcg ctcgaggagg ggcgtacggc ggtgatggcc gacggcacgc gtgttccggt
    14941 gagccgggcg ggcgcggccc ggctgaagga gctcctgccg aggggctgac gccgcggcgg
    15001 gtcgccgcgc cgcgcggctc aggccgcggc ccgcgcgagc actttgacga cgacgacagt
    15061 ccgatcgtcc tgcggctcgt cgcctccggt gtgcgcccgc cacgcggcga ggacggcctc
    15121 gaggacctct cgggcgctct tcccgccgca ggcggcgagc gtcgcctcga gccggtcgaa
    15181 gccgaaggcc tcgctcccgt cgggcgacat cgcctcgacg acgccgtcgg agaggaagac
    15241 ccagacctcg tcgtcgacga gcgggacggt gacggtgacc cgctcgaccg ggcggcccgc
    15301 gccgaggggc ttcgcggggt tctcgaccca ggagacggag ccccgcggcg cgacgcggac
    15361 ggggtagacg tgtccggcgt tcgtgaactc ggcgcgccgg ccctcgaggt cgaaccggac
    15421 gtgggcgagg gtgacgaagg cccgccgctc cgtcgtccgc ctcacttcgt cgtcgaggag
    15481 gccgaggagc gtgggggtgt cggtcccgcc gcgggagagg gcggtgaggc tcgcgagcgc
    15541 cgaggccatg acgatgccgg ccgggaggcc gtgcccggcg acgtcggcga tgacgacggt
    15601 gagcgcggtc tccccttcgc cgaggaagtg gtagaagtcg cccccgatcg cggcggccgg
    15661 gcggaagtcg gcggcgagct cgacgccggg gaaggcgaag tcgggcgcgg ggaggagccg
    15721 ccgctggagg tcgcgagcga gggcgagctc gcggccgagc gcctcggcct cggcgcgcgc
    15781 ggcgacgctc gcctccaggt ggccggccat ctcgttgaag ccggagacga gctccgcgag
    15841 ctggtcgcgt ccgcgcagcc gctcccggac ggcgaagttc cccttctcga tctgggcgaa
    15901 gccgtgagag aggcggcgcg cggcgcgggc gatgcgcgcg acgaggaggg ccgccacgcc
    15961 gaacgcgacg aggtagacga cgagcgtggc cgtcgcgagc cccttgatca gggcgagcgc
    16021 gatcgtcccg gacgccgcgc cggagtcgga gagcttcgcc tgtccgaaga gcgcccgaaa
    16081 ctccgcgggg aaggaggtcc ggaggaggag cgcgccccct tcgtccttca cccgggcgcc
    16141 cgtcccggcg tcgaggaccg gcaggtcgag cgcgaggaac cagacgaccc agccgtcgtc
    16201 gccggggccc ggaggccggg ctccctgcgc cttccgctcc tcccggttga cggaggcgac
    16261 gcgcgtccgc tggcccggcg agtcgatcgt caggctcccc ctccggccct cggcctgggc
    16321 gaccctcgtc ccgtccgcgg cctcggtctt gccgagcccg aagcggacgt tcagccccgc
    16381 ctcgcgcgag agctcgtcct cgagggcggc gtccgccgtc cggtagagga agagggtccc
    16441 tcccttcgtc cggcggccga cggcgaggaa ggagcggtcg cctctccgga ggagcgcgcg
    16501 cagcccgccc tccgggacgg agaccttctc gagggcggtc ccgagcggac cgtccccttc
    16561 gggagcgccc gaggccggca ggagcgccca cccgtcgaac gcgccgctgc cgccagcgag
    16621 ctcgggccgc cgcgccttct cgcgggaggc gaactcggag gccttcgcct ccagcgcgtc
    16681 gagccgcgcg atcaggcgcg cctcggcgcg ccgggcgccc gactggccgg cgaggatgaa
    16741 gagcgcggcc gcggcgagcc cggccacgag gaggagcgga aggacgccga tcaggagata
    16801 cgagacgaag agccggctcc cgacgcgcca gagaaggccg cgcagcaggt gccggaagag
    16861 gctgacgccg aagaccgggg tcgcgaagac gaggaggagg actccggcga cgcgcatcgc
    16921 ctcggcgatc ccggcgtcgc cgccgagaac cgtgaggagg accccggcga agaacgcgag
    16981 gcccgagacc cggaacgtcc gtgtgagggt caacgacgta ctcccgcggc gcgcggccgc
    17041 cctgtcgggt tcgcgcgaga ttacctcgcc gcactgcgga cgcgcaccgg cctaagatgg
    17101 cccgggacgc cgcgccgtga gcctgaccca gatcctcgcc ctggtcgtcg tcgtggccga
    17161 cgtgctcgcg ctcgtccacg cggtgacgcg ccacctcggc gtcacctcga cgctcgcctg
    17221 gatcttcttc atcctcttcc ttccggggat cgggcctctc gcctatttcc tcctcgcgag
    17281 cccgcggatc cggacgaccc ggcggcggaa gcggctggcg ggcgaggccg ttcggacggc
    17341 gatccgccgg aacctggggc acgagccgct cccggccgag atcgacgcct cctcgcccct
    17401 cgcccgctcc gtcctcttcc tcgggacgcg cctgacgggg ctcccgcccc gcgccggcaa
    17461 ccgcgtcgag ctcctcctcg agaacgaggg ggcgttccgc tcgaaggccg cggcgatccg
    17521 cggcgcgacg cggtcggtgt gggccgagta ctacatcgtc gaggacgacg cgaccggccg
    17581 ggccttcctc gccgacctcg cggagcgggc cctcgcgggg tgcgacgtgc ggctcctcta
    17641 cgacgccgtc ggctcctcgg acattcccga cgaagggctc gaggcgctcc gcgcggcggg
    17701 ggggaagacc gcttccttcc tcccgctcaa cccgctccga aggcgctggt ccgcccacct
    17761 gcgcaaccac cggaagctcc tcgtggtcga cgaggagatc gcgtttaccg gcgggatgaa
    17821 cgtcggcgac gagtacgcgc gcgggagccg gctcgcggga ggctcgggcg ggccgctccg
    17881 caaccgcccc tggcgggacg cccacctgag ggtcgaaggg ccggccgtct tcgacctcgc
    17941 aaccgtcttc gccgaggact ggagcttcgc gacgggggac cggctcgtcc cgggccgcat
    18001 cccgatgccc gtcgcgggcg gctccgtcgt cgccgtcctc ccgagcgggc ccgatcagac
    18061 ggcgaacgcg aacgcccacg tctacttctc ggccctctcc ggcgcgctcg agcgctgctg
    18121 gctgacgacc ccctacttcg tccccgacga gccgacggcg cgggccctcg cgaacgccgc
    18181 cttccgcggc atcgacgtcc gcgtcctcgt cccggccgag agcgacgtcc cggtggcccg
    18241 cgcggcgggc cagttcttct acggtccgct gctgcgcgcc ggggtgcgga tcttcgagta
    18301 cgagccggcc gtgctgcacg ccaagaccgt cgtcgtcgac ggctcctggg cgctcgtcgg
    18361 ctccgcgaac ttcgacttcc gcagcttcgc gctcaacttc gagctcggcg ccgtcgtcta
    18421 cgatcgcgcc ttcgccgcgc tcctcgagga gcgcttcacc cgagacctcg ccgactcccg
    18481 ggaggtgacg ctcgccgccg tggagcggcg cggcttcgcc caacgggccc ggatcggcct
    18541 ggcccgcctc ctcgcgccgc tcctctgagg gaactcgccg catgagactt cgctccgtcc
    18601 ccctcctcgc gctcctcctc tccgggctcc cgctcgccgg ggccgacgtc ccgatccacg
    18661 gcatccaggg cgccggggcg tcgagcccgc tcgtcgggca gtccgtgacg acgacgggcg
    18721 tcgtgacggc gctgaggacg aacggcttct acctccaggc cccggacgcg gaggccgacg
    18781 ccgacccggc gacgtcggaa ggggtcctcg tcttcacctc ctccacgccc cccgccgccg
    18841 cggcggtcgg gaaccgcgtc cgcgtgagcg gcagcgtcgt cgagtaccgc ccctcggccg
    18901 acccggcgag cccgcccctc acggagatcg gcgggagcgt ctcggtcacc ttcctctcgg
    18961 cggccaaccc gcttcccgca cccgtcctcc tcacctctgc ggacctgtcg cccgcggggc
    19021 ccgccgaccg cctcgagcgg tacgagggga tgcgcgtcca cgtcccggcg ctcctcgtcg
    19081 tcgccccgac gggcgggtcc gtcgacgagg cgaacgcgac ggggcgctcg aacggggtcc
    19141 tcgtcggcgt cctgccgggg acgccgcgcc ccttccgaga ggccggctcc gacccgctga
    19201 cgccgctccc ggccggaagc ccctgctgcg tcccgcgctt cgacgggaac cccgagcggc
    19261 tccgggtcga caccgacggc ctcgcgggcg ccgcccctct cgacgtcgcc tccgggacga
    19321 cgctcccgga cgtcgtcggc ccgctcgact acggcttccg cacgtacacc cttctcccgg
    19381 agagccggcc cgtcgcaacc ggctcgcccg ccgcgcgcgc cgtccccgcg ccgtcggccg
    19441 acgagctgac ggtcggctcc ctgaacctcc agcgcttctt cgacgccgag gacgacggag
    19501 aggggacgac cctcaacgcg gccgcgttct cgacgcgcct cgcgaaggcg tccctcgtga
    19561 tccggacgct cctgcgcacc cccgacgtcc tcgccgtcgt cgaggtcgag gacctctcca
    19621 cgctccgcgc tctcgcggcg cgcctctcgg cggatgccct cgccgcgggc gatcccgacc
    19681 cggcgtacca ggccttcctc gaggcaggga acgacccggg cggaatcgac gtcggtttcc
    19741 tcgtcgcctc gcgcgtgtcg gtcctctccg tgatccagga ggggaaggcg tcgacctaca
    19801 cggacccgac gaccggcagc gccgcgacgc ggaacgaccg gccccctctc gtcctgaccg
    19861 cccggtgggc cgcccccggc gggaccgatc tcgtcttcac cgtcgtcgcg aaccacctcc
    19921 gctccctcaa cgattccgag gccgactcga cgacgggtcg gcgcgtccgc gcgaagcggc
    19981 aggcgcaggc ggagttcctc gccgcgctcc tcgcgcgcct ccaggccgag gggggcggcg
    20041 cgccgctcct cgcggtcggc gacttcaacg cgttcgaggt gagcgacggc ctcgtcgacg
    20101 tgatcggcac cgtccgcggc gccccggctc cggccgacca ggtcgtcgtc aaccggagcg
    20161 tcgacctcgt cgaccccgac ctcgtcgacc tctgcgcggt cgacgccctg gtccctccct
    20221 ccgagcgcta ctcctacgtc ttcgacggca acgcccagct gctcgaccac gccctcgcgt
    20281 cggcggcgac gctcccgctc gtccgaggcg tcgcctttgc gcgcctcggc gccgacttcc
    20341 ccgaggcgat gcgcggcgat gcggcgcgcc ccgagcgtct caccgaccac gacggcctcg
    20401 tcgtgaggct cggccccgcc gccccgaagg gacgctcgcc cgtcgtcccc gcgcgggacg
    20461 gggagccgcc gtcgccgttc cgctcgcgga ctcccgagac ggcgccgtga ggagcgatct
    20521 cctcgcccgc ctcgaggagg cgcgccgtct ccgccccgga gcgcgcgcgg ccgaagaggc
    20581 gctcgaggcg gccgcccgct tcctcccgcg cgaggcggcg cacctcgccc ggctccacga
    20641 cgcggcgctc ttccttcgcg cgtacccgca gtcgccgcgc gtcctcgccc tcgccgaggc
    20701 gcttctcgcg cgcgtccccg tgcacgtgac gcgcctcgcc ggcgaggggg ccgacctcgg
    20761 gccgctcgac gatccgtccg ccgccgggat cgccggagtc ccggtcgcga tgcccttctc
    20821 ccacgccgcc gcctcgtggc tcgtgcggcg cgacgcgtcg gcgctccgcg tcgactggga
    20881 cgccgaagac ctccccgacc gcctcgccgc gtccctcccg cggctcgtcc cgttcctcga
    20941 ggagcaggcg cgcgtcgacg cgaacgtccc gttccgtgag tacgtcgacg gcgcgcgcgg
    21001 cgcgcggccc gacgccgcct ggctcctcga gaggctcgcc gggctccccc tgcccgattc
    21061 cgtccgcgcc gagctctggg aggcgctctc cctccagctc gcctggacgc cccgcgcttc
    21121 cgactcgcgc ggcctcgccc gcgtcccgtc gcccgcgccc ttctgctccg acgggccgct
    21181 cctctcgcgg cgcgacgtct ccgtcgccgc cgagctcgcc gcggcgccgc cgcccctcgt
    21241 ccgcctctcg cggagcgacg gcgccgccct cctcgacgcg gcgcgcgcgg cgatggccgt
    21301 ccgctaccgg gagctcccgg ccttctccgc aggcgacccg tcggccgtct tccgctgcga
    21361 cgtcggccgc ggcgtctcca tcttcgtcac gggcctcgac cgctccgcgc gcctcccgct
    21421 ccgcgccgcg ttcgggaccc tcttcgcgcg caacggcgtc ctcgtcggct acggcgacct
    21481 ccacgccgcc ctcggccgcg ccgacgtcag cttcaacgtc ttctacgcct tccgcgacgg
    21541 cgagtcggcc tggctctacg cgcggcttct cgccgtcgct cgcgccgtca cccacgccgc
    21601 gcagctctcc gtcgacccgt accagatcgg cctcggcaac gaggaggcga tcgcctcggg
    21661 cgccttctgg ttctaccgga agctcggctt ccgctccgcg gaccccggcc tcgagcggct
    21721 cgcccgccga gaggaggcgc gctcctccgc gtcccccggc cgccggactc cgccggcgac
    21781 cctccgccgc ctcgccgccg ccccgctcct cctcgacgtc ccgggcgaga ccccgttcct
    21841 cccggactcc ttccgcctcg ccggcctcgg cctcgcggct gcacgacgct tcgcggcctc
    21901 gaagctctcg cccgaggcct tccgcgcccg tgccgcccgc cgcgtttcgc gcgccctcgg
    21961 actcgatccc gtccccccgg ccctccgcgc gcccctcgag gctctcgttc ccgtcctcgc
    22021 gcttctggac gacctcacct cctggccgct ctcccaccgc cgcgccgtcg cggcccttct
    22081 ccgcgcgaag gccgcccctg acgagctccg ctacgcgcgc ctctcctcgg cgcacgctcg
    22141 cctcgcggcg tcgatcgccc gggcggtgtc gagctgacgc cggcgcctcc cgtgtggccg
    22201 ggcccgctcc cggcacgacg tcacgagccg gggcgagccg gcgcgccggg gcgacacgga
    22261 aggagtcggg acgcgaccgg cccggcgagc gatgtcctgg cgacgcggcc gggcagccca
    22321 cgcgagcgcc tccgggccgg agtgctcgtc gaggcgttcg gggcggcggc ggcctcggcg
    22381 agcttcaggc ccggctcccg cgacgtgccg aacgcctctt gcacggcgag ccgggccgcc
    22441 gaacgggcgt cggccgggga gccctcctcc gcggagggct cccggcctcg aggtcagcgg
    22501 gtccggaagc gcagccccgc ggccgcgacg agcgcggcca tcaggagcgc gccgaacgga
    22561 ccgaggaccg ggatgtcccg tgcggcctcc accggaacgg gaaagtccgt cggatcggac
    22621 gcgccgggga gacgcgggtc gtccgtcgat ccggccgcgt cgttcgtgcc gtcgccgttg
    22681 gcgtcccagg agagcgctcc ctggttcgag acgacggtcc cggccgccgt cgggaggacc
    22741 gtcgccgtga tcgtgatcgt gaccgaggcg cccgccgcga gggccccgtt ccacgtcacc
    22801 gtcctcgtgc cggcatccgc gctcgccgtc cccgcgctcg cggtcgcgcc gctcagcgcg
    22861 agcgtggtcg ggagaacgtc gaggagctcc ggccccgccg cgtcggcctg cggcccgatt
    22921 ccgtcgttcc ggaggacgat cgcgtaggtc accgtcgcgc ccgccgagaa cgtgccgctc
    22981 cccgtcttcg tcgccgagag cagggcgcc
//
LOCUS       DAOTEZ010000010        22646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_1496_length_22646_cov_6.401177, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000010 DAOTEZ010000000
VERSION     DAOTEZ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 22646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22646
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1496_length_22646_cov_6.401177"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..1035)
                     /locus_tag="P5164_01055"
     CDS             complement(127..1035)
                     /locus_tag="P5164_01055"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRY42506.1"
                     /translation="MEGIIPPLPTPFREDGGLDLGLLSELVAQLAAAPLAGFLALGSN
                     GEAVHLDDDEAERVVAAVRRAAPGRWLLAGTGRLSTRATVDATKRAAGAGADAVLVVT
                     PHFFRGAMSSDALAAHFEAVADASPVPVLLYNVPANTGLDVDAATVARIARHPNVAGV
                     KDSSGDVGKLAELVRLAPAGKPFDVFSGNYGSALPGYAVGTAGSILAAANVAPEGCVA
                     IRQAFLSGRLEEARALHLRLLPMARAVTSRFGVPGLKAALELLGRPAGSTRRPLLPLG
                     PAAREELRSVLAEAGLLTTRAGTSTP"
     gene            1122..1502
                     /locus_tag="P5164_01060"
     CDS             1122..1502
                     /locus_tag="P5164_01060"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRY42507.1"
                     /translation="MRTVETPHAPHPAGHYSQAVVHGGLVFVSGQLPHDVERPGGAPG
                     DAEEQTERALRNVEAILEAAGSGLDRLLSVTVYVADVALWPRVNAAYARVLGAHRPAR
                     AVVPVKELHHGYLVEVQAVAAVRE"
     gene            1511..2383
                     /locus_tag="P5164_01065"
     CDS             1511..2383
                     /locus_tag="P5164_01065"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRY42508.1"
                     /translation="MSKPSPVLPWVAYGVCAVVWGSTYFGIALALESFPPNGMVAVRF
                     SVAALLCLGLGQLRKEPLPSRRELPHLALVGLLLLGICNALVVWSETRLGSGVAAVLA
                     ALTPAWFGVFTAREEPLGARGWTGTALGLAGVTLLAAPWSQKGVDLGGVAALLLASAL
                     WAVGTLHHRKHVTGGGALTNSGLEMLAAAVFGTLAAPLTGGFTSGPVTTKALLAVGYL
                     ALFGSCIAYTAHLYVSRVWSPVRAGSYAYLNPVIAVVLGTAILGEPFDTRMALGMAVI
                     LAGVALVQYRSRAA"
     gene            complement(2402..2785)
                     /locus_tag="P5164_01070"
     CDS             complement(2402..2785)
                     /locus_tag="P5164_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRY42509.1"
                     /translation="MSDTTLFVLIGLVGGVFAGLFGVGGGIVLVPALIYLADFSQHKA
                     TGTSLAVLLPPIGLAATFEYWRHGNVDVKAAAILAATMFAGSWAGAHAANRMKGPHLR
                     LLFGLFVTGMGIYLVRGAAKRLGWL"
     gene            3161..7945
                     /locus_tag="P5164_01075"
     CDS             3161..7945
                     /locus_tag="P5164_01075"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRY42510.1"
                     /translation="MVELEGEPAVEAWIRSRAEGSRSALAASAASTRVAELRVAQARV
                     EELLSAPAIGARVQYRVARAFNGIAVFAEPSRVEAIRALPGVKSVRPLVLHAPANSTS
                     VPHLGVPPAVWQALGNAGDDVKVGIIDSGLDYQHAMFGGTGREADYRANDRTKINDGF
                     FPTARVVGGWDFAGDDYVGGGAAAPDPDPMDCGGHGTHVAGSVGGSGVRSDGSAYTGP
                     WDASVPFASMRIGPGVAPRAKLYALRVFGCSGSTGLTTQAIDWAMDPNDDGDFSDRLD
                     VLNLSLGSEFGSKDDSTAIAAENASRAGIVVVCSAGNSGDTYFVTGSPAAADSAVSVA
                     ASVDAGVTASFLAVLGPSSLAGRMTAGTASFGNAPAAAGTIGPLVAAVPARVCEAVTN
                     GAALAGKIALVDRGDCSFDGKVKRAQDAGAIGVVIASNEDAVIGMAVADPAVASAITI
                     PSVLVSLSDGNRLKASLAEGVTVALFSGSDTLGSFSSRGPRLEATPSGPKPDVAAPGV
                     SITSAATGIVAGVGGPVVNAGNLAASMSGTSMAAPHLTGVMALLRKQRPSWSPAELKA
                     AVMNTAGFDVSLLPPAGSPPQYGPARVGAGRVDPARALATEVIVFDDERPSVVSLSAF
                     LQGAAPATVTRRLRILNKGSAPATLTPGWTTPSAIPGVRVDVPGAPITVPPGGSATFE
                     VAVVLADPSAVTNAPDPTLALRQPSFVSLSNRAWMTEASGFVTLSGAPAGTLRVPLQV
                     VVRGQSAMRAASRAIGAPAEGGSFTIPLTGSGLKTGPLPPVDALSTVSAFELHAESPR
                     SNPEGSARAVADLAFVGAASSIPAGRFDSAATVWFGIATWAEWVSPAEVRFEIEVDRN
                     GDGIVDATVETMSTGDVANPSDPSQSVDPTDVWVARTEQASGAGAKSYRYLNVDPASR
                     GTHLFASNVVVLPASAGADGLGLTAGNARFRYRVKASTSRGGTVEETSWLTFDAEKPG
                     YSILAADATPFHDDLPGSALPARFDPAAAQANGALGVLLLHHHNAYGARAEALTGRNS
                     PPAVTILEPAPGAAFDAAAPVRFRASGTDADAGDTLTYSWSLGDGRTVSDADVSAAFG
                     SAGPRVATVTVTDGAGATATAQVAIEVRAPQAPSGISKLVPVVLDVRGVGGAPFTTEL
                     TLVSRSATAARALLTYTASAGAGSGWAGIDLGPGETRVLPDAIDFLRSQGLAIPDDGS
                     NQIGTLRVTLPGASEPAALFVGGRTSTPGEGGAFGLFYADAVEATGEIVVAGLQQNSA
                     MRSNLAVVNAGPEPVKLRLRLEGANGEVLGILDEPTLPAWGWKQYDQPLLGRAASGRA
                     RVLRLAGGGPFSAYGVLNDAGTSDGSYVPPLLTGGPGAGDRLIPVVLDVKGLGVNRYA
                     TELTLTNLEAAPLELTLAYTAALGGGSGNVPMTLAGGEQRIVPDVMAFLRSSGLAIPA
                     GGANVAGSLLVKVPTGRAADLLAAGARVSTRSSVRDGAFGVFYPGLTHSEAANGTAWV
                     HGLQQDAKTRSNVALVNFGDAGNVTLRLTYYGANGQALGNSEEHALAPGAWLQKNEPL
                     LSRGASAGSVKVERISGTSRFVAYGVLNDAATSDGSYLPMAR"
     gene            complement(8126..8809)
                     /locus_tag="P5164_01080"
     CDS             complement(8126..8809)
                     /locus_tag="P5164_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015623616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY42511.1"
                     /translation="MTAPGVALRARGLTKSFDGNLVRALAGADFDAAAGEVVAVTGPT
                     GCGKSTLLSLLSLLDEPDAGELLVDGMPPASLGPAERWRARNAGIVFQLHHLVPHLTS
                     AENVALPLAGLGLPRAERSRRVAAILDRAGLSHRSGSLASKLSGGERQLCAVARALVG
                     RPRLLLADEPTGSVDSANGERILGLLAGWCRESGATMVLVTHDAAVAATADRIAAMRD
                     GRFLSTAPA"
     gene            complement(8806..9633)
                     /locus_tag="P5164_01085"
     CDS             complement(8806..9633)
                     /locus_tag="P5164_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HRY42512.1"
                     /translation="MRRLPVLPLLALVRAHACATAAALALLGAFLAGEPSSSHSLLPI
                     AAAVFLVTAGGNALNDACDTGPDLVNRRERPIPSGALSRRTAVAVGATCLAAGAVAGS
                     LLTPWCAAICLSNVVLVVAYARCSKRLGLAKDLLVGYLVGSTVLFGALDPARVDAPVA
                     TLAVCAALATAARELLKDVEDVPGDRAAGARTLPLVIGTPRSEALAYLLLGLAVALGF
                     SPPVTEAMSGAYAIAAAGGALVFGLSFLSRRPRTRQLLVMAGSVVEMGAFWAGKVLP"
     gene            complement(9630..10856)
                     /locus_tag="P5164_01090"
     CDS             complement(9630..10856)
                     /locus_tag="P5164_01090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRY42513.1"
                     /translation="MSDRFDLVVVGAGPAGSSAALRAARAGLRTLLLDRATFPRRKTC
                     GDALGGRAVAALRELGLLEEVRRLPGVLVHRVLFTSPEGTELSIDLARAARPDLVTGF
                     VIRREVLDAFLLGKAREAAAEVREGFQVDEVVREGGAVRGVRGRDLATGARREFQADV
                     VLGADGAHSAVARSLGLFRRDRRRTVVATRAYYRGVGGVGDRIELHYLEDVSPGYLWI
                     FPVEDGLANVGIGMLAAPMRRRRVHLVRCLESALVSPRFASRFSGAERVAAPEGADLP
                     VGGSRRVASGAGFLLAGDAAGLVDPFTGEGIANAMWSGLLAAETVVEASRERDFGARS
                     LARYEERLDEGIREELRVAARVQAVARLRPLLDFTIRRAARSPRVRDAICAMIAGTLP
                     RRTLTNPLFYARLLRA"
     gene            10902..11996
                     /locus_tag="P5164_01095"
     CDS             10902..11996
                     /locus_tag="P5164_01095"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY42514.1"
                     /translation="MIAACRALLRRPVRTTLTVLGVAVGYAVWVGISASATGFVARFN
                     GILAATGAEITVQQAGVGFPVLSRIEVGDLERIRAAAGARNVSGVIAAVTRLPNNNQL
                     PVFGVDPADPVATLFEPKEGTALAPGAALLGRAASASAGQRPGGSIEVLPGRRLPVAG
                     IYESAYGFLDGGCVLALGDARRLFEMEGLVSLALVKLEDPAQTNGAIERINRELPHLH
                     ATVSELFFSGYRELELVDRFARFLALAALVVAALGVANTLGMNVVERLPELAILRAVG
                     WSRWRVAGNVLAEGAILALAGALLGVAPAWAFVRATGVAGLQHLASPRPSPGLLLGGV
                     AIVLVSGLLGSLPAIAYLFRARVSAVLRTP"
     gene            11993..13090
                     /locus_tag="P5164_01100"
     CDS             11993..13090
                     /locus_tag="P5164_01100"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY42515.1"
                     /translation="MMPGALRALARRGGRTTAAIAGVAIGFAAWISLVSAADDLPRRF
                     GARVAATGAEMAVRQAGVGMAILSRVKAADVPRIREAAGARWASGVLVQLIRRRDGSQ
                     LPVFGVDLESPLASIFTLEEGRRLVPGSREVLVGRSAAESLGRSAGSELEILPGDARR
                     VRGVFASHNAFVDGGCALELGEAQRLFGMEGLVSLVLVKLDDPARLREAIGLVKGQQP
                     HLHATASELYFAGFHELDAVASYARSLGLAALVVAVLVVASVLARNVAERRQELAVLR
                     AVGWSRTRVAGTVLAEGALLATLGGAVGLAGAWLFLRAASVAGLSPWLAPAIPPRLVA
                     EGVFLLALGVGLGCVPALVTCWATPPSAALR"
     gene            complement(13087..16368)
                     /locus_tag="P5164_01105"
     CDS             complement(13087..16368)
                     /locus_tag="P5164_01105"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HRY42516.1"
                     /translation="MTKAGDRSSSRGQMLPWLGLAASTLAVMLLAGGTRLLPDSPLVP
                     GNAARLAATAGTSVFVPASAHATGANGSNWRTDVEVHNPGSTTASFTVQLLRRDANNS
                     APASKSYSLAARQSRRFADMLLTEFGLDGAAALRFVVASGSILVSSRTYNLLGQNPWG
                     LPQGASFGQYVPGLDAAGAIGFGQEGRLIQLTQRPAGDLDGFRTNLGIVNATASALDV
                     KIDFYRADGTWLGKREGGETNLPPYGFRQLDQAFSAWGTLADGYAVVKPLTAGGALFA
                     FATVIDNHVSGDPVFIPAVRRTSGSTSPTPTPTRTPTATPTRTPAPTPSPTPSGGLPN
                     LIVYKPSTWPACVVANYQSGCCTSSACCSPFLSTYYTTHIQFAVGNTGSTRVTAPMQF
                     SLSIDGVVVGLANWSNPEGIEPGEGWLLSWPPFTDPVSEGMHTLTIRADSASAIAESN
                     EADNSCGSSATWTSIVFFTGTSAASEVPAEDAITAVAPFRLEAGGRVPLVAAPGTVYV
                     PASAHASGLNGANWRTDLEVHNPGSTAVGFQVTLLPQASDNGGTPPSRGFSLGPQQSV
                     RYGDVLSSLFGYTGAAALRVEPAGGATLLVTSRTYNQIGPNSVGLPVGASFSQFVPGL
                     DENASAIPYGEEGRLIQLTHRASSTLADFRTNIGYLNTTGSTIDLRIDLYAKDGTLLG
                     TIQDDRTHLRPYESRQITEIFGAFASSLDDGYAVIRTTTPAGRFLAFATVIDNHITGD
                     PIFVPAIRVSGSTSPTPTPTPTPSATTIPSGPNAIPGAEILNGAMAGLGNLGSGTASI
                     ADIANTGKLNGIDAALTQIANLSPSTTRKSGNGLVIDYGAGYKAKDGAVYKGSATITT
                     SSFNVAGNRVTWTGVMTPNGLTKDGRALGSGSASWTIDYVADGSKVVGDASGSATSTS
                     NPADSGSGSAHFDSSVCARYPVSGSITMTQGGKTTTAGFTNACDGSYTLSGTGLRYDT
                     LDLQVKRCDGTYRSEHFRVALVETGGAILKDPVCGNRALSGFSNVRAWGTVTDTTVKL
                     SFFGQIGTTVHSYGGTYQGTRSGPGQPFSGSASYTVVGACAVSYTASGPDVSLTSQPS
                     SACNY"
     gene            complement(16522..18771)
                     /locus_tag="P5164_01110"
     CDS             complement(16522..18771)
                     /locus_tag="P5164_01110"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding and patatin-like
                     phospholipase domain-containing protein"
                     /protein_id="HRY42517.1"
                     /translation="MHERAARPGDGASLFSFPEYDALPAEDRAAVDALVSTVRVPRGE
                     TVFRRGEPGDAMFVVTGGRLRVTGTTPSGAEVTLGEIGPGEWTGEMAILTGQPRSATL
                     TAIEESVLVRLPREGWDELARRYPAVASRLTEAIGPRLRRAQLLRVWEELFGITEPDA
                     FRDLEEAVEWRRVAAGELLVRQGDPSDAMYVVVTGRFRTTVRGADGTETVVRETGAFS
                     TVGELGLLADQPRSASVVALRDGEVLRLGRDAFRGFALKRPEALLRVASIVAGRQAGS
                     SGPKSGTASPVPAPDGLTIAVLPLGPSASASALARTLASRLEALGSTLLLDAAGLDAR
                     HGRPGVAEAPLAGPLGLAVDVSLRELEDRTRHLVLDAGSGGEAWRERCLRRADRVLLV
                     ADAAADAAPGAIDARLAGMALPARKELVLIHAPETTEPRGTAAWLAGRDLADHHHVRP
                     GNARDEVRLARRLAGRGVALVLGGGGARGVAHIGVIRALEEAGVDVDLVAGTSMGAVV
                     GASFALHAASAPLLALAETFASPGKIFDRTLPLVSLMSGGKVLRVMKGLYGDRLIEDL
                     WTPFFCVSTNLTRARVDVDRSGPLWRAVRKSMSIPGVFPPVIEEGDVVVDGGVVDNFP
                     VERMASRLDCGTVIGVNVAPAVDKVKPYRFGPELSGWKVLRGRILPFVRKTRAPSLVG
                     SILRTQEINSVMALRSGLRFVDLLVEPSVDRFRLNEYGRWEELVEAGYGAAKEALPAF
                     LASRPAAAG"
     gene            complement(18930..19589)
                     /locus_tag="P5164_01115"
     CDS             complement(18930..19589)
                     /locus_tag="P5164_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42518.1"
                     /translation="MSRRDATVSAATIAILVLGPLACRKAEAPAADPPPAAAAAAPAP
                     ADPSCKDGSRRDHPDGKLKECLLAADWVYGPYTCRMNWSVVLHPDGKLKECKIAGTAT
                     FDGLVVKESLALYEDGRFRRGVVAAAKTFGDVEAREGDWVTLYPGGALNRLELFGGPR
                     TIQGYPCKGSENYFHENGRLKKCTLGEVFKQGEKELPAGTLLCFDANGQPTEPCTLMT
                     P"
     gene            complement(19675..20187)
                     /locus_tag="P5164_01120"
     CDS             complement(19675..20187)
                     /locus_tag="P5164_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42519.1"
                     /translation="MPNRFAPVLVATLAAFAAGTLTAGAASPAPIELHGLEATPPVSF
                     SLPESAGLDAVRVVHPPDAKAGGEKLSLLAVAFPKDSGMSDAELLEYVKTTFLATSAA
                     GRPVERTLLGRKVKGLALEKKIPSPSLAQVYVATKKGGDKVVLAFVFAPGFANEAEAA
                     IAAITGSLKE"
     gene            complement(20226..20765)
                     /locus_tag="P5164_01125"
     CDS             complement(20226..20765)
                     /locus_tag="P5164_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42520.1"
                     /translation="MKATFVPLALVLALAPLSGSAADCPRATAVEKQSKNAAYKPVAP
                     FDLKALTKTKATKSAFGITVFIANADFDVKAAAARLDTSPVKGKGQVSLAITFMNASK
                     PVVAGEYAMRANVWDPMNVRGDLGFGKDDGTPVVYGLGLKSGKATLVEVTDKRVCGTF
                     DVVGQDGPFKGSFVATVVK"
     gene            complement(20833..21426)
                     /locus_tag="P5164_01130"
     CDS             complement(20833..21426)
                     /locus_tag="P5164_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42521.1"
                     /translation="MKPSCALLLLAFLAAGPAGAQTPDDRGFDPTGVWFGQHGPLALM
                     RAGDTLTFSYSGVFGATAHMCDGIGVAGFVGDGRWEYVDEQGTVAFTTKGGKVTMEAT
                     KGIASFCGANWPGDTFAKEGWKPSFNCTVVAPKARFLTAAPSPEPRKGYVVKGNVVEA
                     VGLVNEGTPGWLLVRYVGKKQTTAGLLERDALECREE"
     gene            complement(21449..21907)
                     /locus_tag="P5164_01135"
     CDS             complement(21449..21907)
                     /locus_tag="P5164_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42522.1"
                     /translation="MGLVFALAIVAVIGGVVWAAIRGQGKDWDVAAEIDRGVMADYGR
                     LVGEGRFAEAWEKCLTASYRDEVPREKFVAAHEKRRTETGALRGCRLLRADLTRNLFS
                     KTRELHLLYELTYPGRVEREYAKVNDADGSWRIEGTYHLDAGETYDFLLW"
     gene            22220..>22646
                     /locus_tag="P5164_01140"
     CDS             22220..>22646
                     /locus_tag="P5164_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42523.1"
                     /translation="MSAVAPGGGGSGAGDSVRERTPIRTVAYVISAVGALVGPVSFLA
                     FPGFGAWGSEDLEGAGVLALTALLMASPFAAFFLAARLSRTKAGAAVVAALALVAVLF
                     GALFYVDAALRRDFVLLLASVGVPAVQWLMALPAVVAALV"
BASE COUNT         2922 a         8170 c         8425 g         3129 t
ORIGIN      
        1 aaaggctcaa atcgacctcg tcacggcaag gaccgtgccc ctgattctct cgtcacggac
       61 gggccggggc aagcgaggcc ggccggacgc gtggagggcg agggccggcg gtcgcgcccc
      121 cctggttcac ggcgtcgacg tgccggctcg ggtcgtgagg aggccggcct cggcgagaac
      181 ggagcgaagc tcctcccgcg cggccgggcc gagcgggagg agggggcggc gggtcgagcc
      241 cgcgggccgg ccgagcagct ccagcgccgc tttgaggccg gggacgccga atcggctcgt
      301 cacggcgcgg gccatcggaa ggagacggag gtggagagcg cgcgcctcct cgagccgccc
      361 cgagaggaac gcctgccgga tcgcgacgca cccttcaggg gcgacgttcg cggcggcgag
      421 gatcgagccg gccgtcccga ccgcgtagcc ggggagggcc gatccgtagt tcccggagaa
      481 gacgtcgaac ggcttcccgg cgggcgcgag ccggacgagc tcggcgagct ttccgacgtc
      541 gcccgagctg tccttcacgc ccgccacgtt cgggtggcgc gcgatccgcg ccaccgtcgc
      601 ggcgtcgacg tcgaggccgg tgttggccgg gacgttgtag aggaggaccg ggaccgggga
      661 ggcgtcggcg accgcctcga agtgcgccgc gagcgcgtcg gacgacatcg cgccccggaa
      721 gaagtgcggc gtcacgacga ggacggcgtc ggcgcccgcg ccggccgccc gcttcgtcgc
      781 gtcgaccgtc gcgcgagtcg agagacggcc cgtgccggcg aggagccagc gaccgggagc
      841 ggcgcgccga accgcggcca cgacccgctc ggcctcgtcg tcgtcgaggt gcacggcctc
      901 tccgttcgag ccgagggcga ggaagcccgc gaggggagct gcggcgagct gcgcgacgag
      961 ctcggagagg aggccgaggt cgaggcctcc gtcctcccgg aacggagtcg ggagcggggg
     1021 gatgatccct tccatccgta gatcttatcg acccgcggtc cgtccggccg cgtcggaggc
     1081 cgcggttgac gcctcgccgg ggcggcgcag aatccggcgc catgaggacc gtcgagacgc
     1141 cccacgcgcc gcaccccgcc gggcactact cgcaggccgt cgtccacggc ggcctcgtct
     1201 tcgtctccgg acagcttccg cacgacgtcg aacgtccggg cggcgcgccg ggcgacgcag
     1261 aagagcagac ggagcgcgcg ctccggaacg tggaggcgat cctcgaggcc gcggggagcg
     1321 gtctcgaccg gctgctgtcg gtgacggtct acgtcgccga cgtcgcgctc tggccgcgcg
     1381 tgaacgccgc ctacgcgcgc gtcctcggtg cgcaccgtcc ggcccgtgcc gtcgtgcccg
     1441 tgaaggagct ccaccacggc tacctcgtcg aggtccaggc cgtggcggcg gtccgggaat
     1501 agacgcccgg gtgtcgaagc cctctcccgt cctcccgtgg gtcgcctacg gcgtctgcgc
     1561 cgtcgtctgg gggagcacct acttcgggat cgcgctcgcc ctcgagtcgt tccccccgaa
     1621 cggaatggtc gcggtgcggt tcagcgtcgc cgccctcctc tgcctcgggc tcggccaact
     1681 ccgcaaggag ccgcttccct cgcgccgcga gctcccgcac ctggccctcg tcggcctcct
     1741 cctcctcggg atctgcaacg cgctcgtcgt ctggtcggag acgcgtctcg gctcgggcgt
     1801 ggcggccgtc ctcgcggcgc tgacgcccgc ctggttcggc gtcttcacgg cgcgggagga
     1861 gcccctcggc gcgcgcggct ggacgggcac ggcgctcggc ctggccggcg tcacgctcct
     1921 cgcggccccg tggtcccaga agggggtcga cctcggcggc gtcgcggcgc tcctcctggc
     1981 gagcgcgctc tgggccgtcg ggacccttca tcaccggaag cacgtcaccg gcggcggagc
     2041 gctcacgaat tccgggctcg agatgctcgc ggcggccgtc ttcgggacgc tcgcggcgcc
     2101 gctcaccggc ggcttcacga gcggccctgt gacgacgaag gccctcctcg cggtcggcta
     2161 cctcgcgctg ttcggctcgt gcatcgccta cacggcgcac ctctacgtct cgagggtctg
     2221 gagccctgtg agggccggca gctacgccta cctgaatccg gtcatcgccg tcgtcctcgg
     2281 cacggcgatc ctgggggagc cgttcgatac gcggatggcc ctggggatgg cggtgatcct
     2341 cgcgggcgtg gcgctcgtcc agtaccgctc ccgcgccgcc tgacggggcg gacgcgcttc
     2401 ctcagagcca gccgagccgc ttcgcggccc cccggacgag gtagatcccc atgccggtga
     2461 cgaacaggcc gaagaggagc cgcaggtgcg ggcccttcat ccggttcgcc gcgtgcgccc
     2521 cggcccacga gccggcgaac atcgtcgccg cgaggatcgc ggcggccttc acgtcgacgt
     2581 tgccgtgcct ccagtactcg aaggtcgccg cgaggccgat gggcggcagg aggaccgcga
     2641 ggctcgtccc ggtcgccttg tgctgggaga agtcggcgag gtagatcagg gcggggacga
     2701 ggacgatgcc cccgccgacg ccgaagagcc cggcgaaaac gcctccgacc agcccgatca
     2761 gtacgaagag agtcgtgtcg ctcacggggg tctgacctcc tgacgccggg gccctctcgg
     2821 gtccgcgtcg ctccacggtg acattccgag gcgagggctt caaggggacc gtcgtggcct
     2881 ggtttgattc cgggccgggg ccggtccatc ctccccgcgc gggaggcctc tcccggcagg
     2941 gggcgtgatg ctcgtttcca gaaccgattc caacggccgt gcacgggacg cgcggcatga
     3001 cgcgacggcc cccggacccc ggggggcgca cgatgcggtt cgcgccgcgg gggaacgcgg
     3061 ccggcgtgcc ggccggggac gcggcctgct gctcggagcg agccttctcc tcgctccgtc
     3121 gctcctcgcg gcggggcccg ggaccgaccc gaggcagtgg atggtcgagc tcgagggcga
     3181 gccagccgtc gaggcctgga ttcgaagccg cgccgagggc tcgaggtcgg cgctcgccgc
     3241 gtccgcggcg tcgacccgcg tcgccgagct ccgcgtcgcg caggcgcgcg tcgaggagct
     3301 gctctccgcg ccggcgatcg gcgcccgggt ccagtaccgg gtcgcgcgcg ccttcaacgg
     3361 gatcgccgtc ttcgccgagc cctcccgcgt ggaggcgatc cgggccctcc cgggcgtgaa
     3421 gtcggtgcgg ccgctcgtcc tccacgctcc ggccaactcc acgagcgtgc cccacctcgg
     3481 cgtgccgcct gcggtctggc aggccctcgg gaacgccggc gacgacgtga aggtcggcat
     3541 catcgacagc ggcctcgact accagcacgc gatgttcggc gggaccggcc gggaggccga
     3601 ctacagggcg aacgaccgga cgaagatcaa cgacggcttc ttcccgaccg cgcgcgtcgt
     3661 cggcgggtgg gacttcgccg gggacgacta cgtcggaggg ggggccgcgg cgcccgaccc
     3721 cgacccgatg gactgtggcg gacacggcac gcacgtcgcg ggaagcgtcg gcggctccgg
     3781 agttcggtcc gacggctcgg cctacacggg gccctgggac gccagcgtcc ccttcgcgtc
     3841 gatgcggatc ggccccggcg tggctccgcg cgcgaagctc tacgcgctcc gcgtcttcgg
     3901 ctgctcgggc tcgaccgggc tgacgaccca ggcgatcgac tgggcgatgg acccgaacga
     3961 cgacggcgac ttctccgacc ggctcgacgt cctgaacctc tcgctcggga gcgagttcgg
     4021 ctcgaaggac gactcgaccg ccatcgccgc cgagaacgcc tcgcgcgccg ggatcgtcgt
     4081 cgtctgctcg gccggcaact cgggcgacac ctacttcgtc accgggagcc cggccgcggc
     4141 cgactcggcg gtctccgtcg ccgcgtccgt cgacgcgggc gtgacggcgt ccttcctcgc
     4201 ggtcctcggc ccgtcctccc tcgccgggcg gatgactgcc ggaacggcgt cgttcgggaa
     4261 cgcgcccgcg gcggccggga cgatcgggcc gctcgtcgcg gcggtcccgg cgcgggtctg
     4321 cgaggccgtt acgaacgggg ccgcgctcgc ggggaagatc gcactcgtcg accgggggga
     4381 ctgctccttc gacgggaagg tgaagcgggc tcaggacgcc ggggcgatcg gcgtcgtcat
     4441 cgcgagcaac gaggacgcgg tcatcgggat ggcggtcgcc gacccggccg tcgcctccgc
     4501 gatcacgatc ccgtccgtcc tcgtttcgct ctccgacggg aaccggctca aggcgtcgct
     4561 cgccgagggc gtcaccgtcg cgctcttctc cggctcggac accctcggct cgttctcctc
     4621 ccgagggccg cgtctcgagg cgacaccttc cggcccgaag ccggacgtcg ccgctccggg
     4681 cgtgtcgatc acgtccgccg cgaccgggat cgtcgccggc gtcgggggac ccgtggtgaa
     4741 cgccgggaac ctggccgcgt cgatgtcggg gacctccatg gcggccccgc acctgacggg
     4801 cgtgatggcg ctcctccgga agcagcgccc ctcctggagc ccggccgagc tcaaggccgc
     4861 ggtgatgaac acggccggct tcgacgtgag cctcctgccg ccggcgggct ccccgccgca
     4921 gtacggtccg gcgcgcgtcg gtgcgggacg ggtcgacccg gcgcgggcgc tcgcgaccga
     4981 ggtgatcgtc ttcgacgacg agcggccttc cgtggtgagc ctctcggcgt tcctccaggg
     5041 ggccgcgccc gcgacagtga cgcggcggct gcgcatcctg aacaagggct ccgcgccggc
     5101 gacgctgacg ccgggatgga cgactccgtc cgcgatcccc ggcgtgcgcg tggacgtccc
     5161 cggcgcgccg atcaccgttc cgccgggcgg gtcggcgacg ttcgaagtgg ccgtcgtcct
     5221 cgccgacccg tcggcggtga cgaacgcccc cgacccgacc ctcgcgctcc ggcagcccag
     5281 cttcgtgtcg ctctcgaacc gagcctggat gaccgaggcg agcggattcg tcacgctttc
     5341 gggggccccc gcggggacgc tccgcgtccc gctgcaggtc gtcgttcgcg gccagtccgc
     5401 gatgcgggcc gcctcgaggg cgatcggcgc gcccgcggag ggcggcagct tcacgatccc
     5461 gctcacgggg agcggcctga agaccggccc gcttccgccc gtcgacgcgc tctcgaccgt
     5521 ctcggcgttc gagctccatg ccgaaagccc gcggtcgaac cccgagggga gcgcacgggc
     5581 cgtcgccgac ctcgcgttcg tcggggccgc gagctccatt cccgcgggcc gcttcgactc
     5641 cgccgcgacc gtctggttcg ggatcgcgac ctgggccgag tgggtgagcc cggccgaggt
     5701 ccggttcgag atcgaggtcg acaggaacgg cgacggcatc gtcgacgcga cggtggagac
     5761 gatgtcgacg ggcgacgtgg cgaacccgtc ggacccgtcg cagtcggtcg acccgaccga
     5821 cgtctgggtc gcccgcacgg agcaggcctc cggcgcgggc gcgaagagct accgctacct
     5881 gaacgtcgat cccgcctccc gcggcacgca cctcttcgcc tccaacgtcg tcgtcctgcc
     5941 cgcctctgcc ggcgcggacg gcctcggcct caccgccggg aacgcccggt tccgctaccg
     6001 ggtgaaggcc tcgacgtcgc gcggcggcac cgtcgaggag acctcctggc tgacgttcga
     6061 cgcggagaag ccgggctact cgatcctcgc ggccgacgcg acgccgttcc acgacgacct
     6121 cccggggtcg gcccttccgg cacgcttcga tccggccgcg gcgcaggcga acggcgccct
     6181 cggcgtcctt ctgcttcacc accacaacgc ctacggggcg cgcgccgagg ccctgaccgg
     6241 gaggaacagc ccgcccgccg tgacgatcct cgagccggcg ccgggcgccg cgttcgacgc
     6301 ggccgcgccg gttcgcttcc gcgcctccgg gaccgacgcc gacgcgggcg acacgctgac
     6361 ctattcctgg agcctgggcg acgggcggac cgtctccgac gccgacgtct ccgccgcctt
     6421 cggcagcgcc gggccgcggg tcgccaccgt gaccgtgacc gacggcgccg gcgcgaccgc
     6481 gaccgcgcag gtcgcgatcg aagtccgcgc gccgcaggcg ccgtccggca tctcgaagct
     6541 cgtccccgtc gtcctcgacg tgaggggggt cggcggggct cccttcacga cggagctgac
     6601 gctcgtctcg cggtcggcga ccgccgcgcg ggcgctcctc acctacacgg cctcggcggg
     6661 agccggctcc gggtgggccg ggatcgacct cggccccggc gagacgaggg tcctgccgga
     6721 cgcgatcgac ttcctccgct cccaggggct cgcgatcccc gacgacggct ccaaccagat
     6781 cgggacgctt cgcgtgacgc tccccggagc atcggagccg gccgccctct tcgtcggcgg
     6841 ccgcacttcg accccgggcg agggcggcgc gttcgggctc ttctatgccg acgccgtcga
     6901 ggcgacgggg gagatcgtcg tcgcggggct ccagcagaac agcgccatgc gctcgaacct
     6961 cgcggtcgtg aacgcggggc cggagccggt gaagctgcgg ctgaggctcg agggcgcgaa
     7021 cggagaggtt ctcggaatcc tcgacgagcc gacccttccg gcctggggct ggaagcagta
     7081 cgaccagccg ctcctcggcc gggcggcctc cgggcgcgcc cgggtgctgc gccttgccgg
     7141 cggtgggccc ttctcggcgt acggcgtcct gaacgacgcg gggacgtccg acgggtctta
     7201 cgttccgccg ctgctgacgg gcggtccggg cgcgggggac cgcctcatac cggtcgtcct
     7261 cgacgtcaag gggctcggcg tcaatcgcta cgccacggag ctgacgctca cgaacctgga
     7321 ggctgcgccg ctcgagctca cgctcgccta cacggccgcc ctcggcggcg gctcgggtaa
     7381 cgtcccgatg acgctcgcgg gcggagagca gaggatcgtc cccgacgtga tggcattcct
     7441 tcgcagctcc gggctcgcaa tcccggcggg cggcgcgaac gtcgccggct cgctcctcgt
     7501 gaaggtcccg acgggcaggg cggcggacct ccttgccgcc ggggctcgcg tctcgacccg
     7561 ttcgagcgtc cgcgacgggg ctttcggcgt cttctacccg ggcctcaccc actccgaggc
     7621 ggcgaacggg acggcctggg tccacggcct ccagcaggac gcgaagacgc gctccaacgt
     7681 ggccctcgtg aacttcggcg acgcggggaa cgtcacgctc cgcctgacgt actacggcgc
     7741 gaacggccag gctctcggca attccgagga gcacgccctt gctcccggcg cctggctcca
     7801 gaagaacgag ccgctcctct cgcgcggtgc gtccgccggc tcggtgaagg tcgagcggat
     7861 ctcgggcacg tcgcgcttcg tcgcctacgg cgtcctgaac gacgccgcga cctcggacgg
     7921 aagctacctg ccgatggcgc gctgaggacg ctgcgcgcgc ccgtccctcg gggggcgggc
     7981 gcgcgcggaa gcgccccggc cgagccgaca gggtccgcga aagtcgtcag gaggtcgaag
     8041 agcaggccgt acagccgcag gacggcctcg gtccccggga aggagcgcag gcagaatccg
     8101 tgggcggcgg aaccgagccg atgagtcagg ccggtgccgt ggagaggaac cggccgtccc
     8161 gcatggcggc gatccggtcc gcggtcgcgg cgacggccgc gtcgtgagtc acgaggacca
     8221 tcgtggcgcc gctctcgcgg caccacccgg cgagcaggcc gaggattcgc tcgccgttcg
     8281 cggagtcgac gctcccggtc ggctcgtcgg cgagaaggag cctcggccgg ccgacgagcg
     8341 cgcgcgcgac cgcgcagagc tgacgctcgc ccccggagag cttcgaggcg agcgatccgg
     8401 accgatgcga gagaccggcg cgatccagga tggcggcgac gcgccgcgac cgctcggcgc
     8461 gcggaaggcc gagacccgcg aggggcaggg cgacgttctc ggccgacgtc aggtgcggca
     8521 cgaggtggtg gagctggaag acgatccccg cattccgcgc acgccaccgc tcggccgggc
     8581 cgagcgaggc gggaggcatc ccgtccacga ggagctctcc ggcgtcgggc tcgtcgagaa
     8641 gcgagaggag cgagaggagc gtcgacttcc cgcagcccgt gggtcccgtc accgcgacga
     8701 cctcgcccgc cgcggcgtcg aagtccgctc cggcgagcgc gcggacgagg ttcccgtcga
     8761 agctcttcgt gagacctcgc gcccggagcg cgacgccggg agcggtcacg gcagcacctt
     8821 cccggcccag aaggcgccca tctccacgac cgaccccgcc atcacgagga gctggcgggt
     8881 cctcgggcgc ctggagagga acgagaggcc gaagacgagc gcccctcccg ccgccgcgat
     8941 cgcatacgcc ccgctcatcg cctccgtgac cggcggcgag aagccgagtg cgacggcgag
     9001 gccgagaagg aggtacgcga gcgcctcgct ccggggcgtg ccgatgacga gcggcagcgt
     9061 ccgcgctccg gccgcgcggt cgcccggaac gtcctcgacg tccttcagga gctcgcgggc
     9121 cgcggtcgcg agggccgcgc agacggcgag cgtcgcgacc ggcgcgtcca cgcgggcggg
     9181 gtcgagcgcg ccgaagagga ccgtcgaccc gacgaggtac ccgacgagca ggtccttcgc
     9241 gaggccgagg cgcttcgagc agcgggcgta ggcgacgacg aggacgacgt tcgagagaca
     9301 gatcgccgcg caccacggcg tgaggagcga acccgcgacc gcgccggccg cgaggcaggt
     9361 cgcgccgacc gcgacggccg tccggcggga gagcgcgccc gaggggatcg ggcgctcgcg
     9421 gcgattgacg aggtccggtc ccgtgtcgca ggcgtcgttg agggcgtttc cccccgcggt
     9481 cacgagaaag accgctgcgg cgatcgggag gagcgaatgg gaagacgacg gctcgccggc
     9541 gaggaacgcg cccagcagcg cgagcgcggc cgcggtcgcg caggcgtggg cgcggacgag
     9601 ggcgaggagc ggcaggacgg gaaggcgtct catgcgcgga ggagccgtgc gtagaagagg
     9661 ggattcgtca gggtgcgccg cgggagcgtg ccggcgatca tcgcgcagat cgcgtccctg
     9721 acgcgggggc tccgcgccgc cctccggatc gtgaagtcga ggagaggccg gaggcgcgcg
     9781 accgcctgga cgcgagcggc cacgcgaagc tcctcgcgga ttccctcgtc cagccgctcc
     9841 tcgtagcgcg cgaggctcct cgcgccgaaa tcccgctcgc gcgacgcctc gaccaccgtc
     9901 tccgcggcga gaaggcccga ccacatcgcg ttcgcgatcc cctcgccggt gaacgggtcg
     9961 acgaggccgg cggcgtcgcc ggccagcagg aagcccgcgc cggacgcgac gcggcgcgag
    10021 ccgccgaccg ggagatcggc tccctccggg gcggcgacgc gctcggcgcc ggagaagcgc
    10081 gacgcgaacc ggggagagac gagcgcggac tcgaggcagc ggacgaggtg gactctcctc
    10141 cggcgcatcg gggcggcgag catgccgatc ccgacgttcg cgagaccgtc ctcgacgggg
    10201 aagatccaga ggtagcccgg gctcacgtct tccaggtagt ggagctcgat ccggtcgccg
    10261 accccgccga cgcccctgta gtaggcccgc gtcgcgacga cggtgcgccg ccggtcgcgg
    10321 cggaacaggc cgaggctgcg cgcgacggcc gagtgggcac cgtcggcgcc gaggacgacg
    10381 tccgcctgga actcgcgccg ggcgccggtc gcgaggtcgc gaccgcgcac cccgcgcacc
    10441 gcgccgccct cgcgcacgac ctcgtcgacc tggaagccct ctcggacctc ggcggccgct
    10501 tcgcgcgcct tcccgagaag gaacgcgtcc aggacctcgc ggcggatcac gaagccggtc
    10561 acgaggtcgg gacgggcggc gcgcgcgagg tcgatcgaga gctccgtccc ctccgggctc
    10621 gtgaagagga cgcggtggac gagaacgccg ggaaggcgcc ggacctcctc caggagcccg
    10681 agctcgcgga gcgccgcgac ggcccggccg ccgagcgcgt ctccgcaggt cttccttcgg
    10741 ggaaaggtgg cccggtcgag gaggagcgtc ctgagccccg cccgggccgc ccggagcgcc
    10801 gccgaagaac cagcaggccc cgcgccgacg acgacgaggt cgaaccggtc gctcacgaac
    10861 tttcattatc gtcgaccggc gacgatgggc atactcgggg cgtgatcgcc gcgtgccgcg
    10921 cgctcctccg gcgtcccgtc cggaccacgc tgacggtgct cggggtcgcc gtcgggtacg
    10981 cggtctgggt cggcatcagc gcatcggcca cggggttcgt cgcacggttc aacgggatcc
    11041 tcgcggcgac cggggcggag atcacggtgc agcaggcggg agtcggcttc ccggtccttt
    11101 cgcggatcga ggtcggggac ctggagcgga ttcgcgcggc cgcgggcgcg cggaacgtct
    11161 cgggcgtcat cgcggcggtg acgcggctgc cgaacaacaa ccagctcccg gtcttcggcg
    11221 tcgacccggc cgatcccgtg gcgacgctct tcgagccgaa ggaggggacc gcgctcgcgc
    11281 ccggcgcggc gctcctcggg cgggcggcct cggcgagcgc cgggcaacgg ccgggcggca
    11341 gcatcgaggt cctccccggt cggcggctgc cggtggccgg catctacgag tcggcgtacg
    11401 ggttcctcga cggcggatgc gtcctcgcgc tcggtgacgc ccggcgcctc ttcgagatgg
    11461 agggcctcgt cagcctcgcg ctcgtgaagc tcgaggaccc cgcgcagacg aacggcgcga
    11521 tcgagcggat caaccgcgag ctgccgcacc tgcacgcgac ggtctcggag ctcttcttct
    11581 ccggctaccg ggagctggag ctcgtggacc gcttcgcccg tttcctcgcg ctcgcggccc
    11641 tcgtcgtggc cgcgctcggc gtcgcgaaca cgctcgggat gaacgtcgtg gagcgcctcc
    11701 cggagctcgc gatcctgcgc gccgtcgggt ggagccgctg gcgcgtcgcg gggaacgtcc
    11761 tggccgaggg cgcgatcctc gccctcgcgg gcgcgctcct cggggtcgcc ccggcctggg
    11821 ccttcgtccg ggcgaccggc gtcgcgggcc tccagcacct cgcctcgccg cgcccgtccc
    11881 cggggctcct tctcggcggg gtcgcgatcg tcctcgtctc gggcctcctc ggcagcctcc
    11941 cggccatcgc gtacctcttc cgggcgaggg tgtcggccgt gctccggacc ccatgatgcc
    12001 cggagcgctg cgcgccctgg cgcggcgggg cgggcgcacg acggcggcga tcgcgggcgt
    12061 cgcgatcggc ttcgcggcgt ggatctccct cgtctcggcc gcggacgacc tcccgcggcg
    12121 gttcggcgcg cgcgtcgccg cgacgggcgc ggagatggcc gtgcggcagg cgggcgtggg
    12181 gatggcgatc ctctctcgcg tgaaggccgc cgacgtgccg cgcatccgag aggccgccgg
    12241 cgcgcgctgg gcctcgggcg tcctcgtcca gctgatccgc cgccgcgacg gatcgcagct
    12301 ccccgtcttc ggcgtcgacc tcgagtctcc gctcgcgagc atcttcacgc tggaggaagg
    12361 gcgccgcctc gttcccggct cccgggaggt cctggtcggc cgttcggcgg cggagtctct
    12421 cggtcgctcc gccgggtcgg agctcgagat cctgccgggg gacgcccgtc gagtccgtgg
    12481 cgtcttcgcc tcgcacaacg ccttcgtcga cggaggctgc gcgctcgagc tcggggaggc
    12541 gcagcgcctg ttcggcatgg aggggctcgt cagcctcgtc ctcgtgaagc tcgacgaccc
    12601 ggcccgcctc cgggaggcca tcggcctcgt gaaggggcag cagccgcacc ttcacgcgac
    12661 cgcctccgag ctctacttcg cggggttcca cgagctcgac gcggtcgcgt cgtacgcgcg
    12721 ttccctgggc ctcgcggcgc tcgtcgtcgc ggtcctcgtg gtcgcgagcg tcctcgcgcg
    12781 gaacgtcgcc gagcgacggc aggagctcgc cgtcctccgg gcggtcgggt ggagccgaac
    12841 gcgcgtcgcc gggaccgtcc tcgccgaagg cgccctcctc gcgacgctcg ggggcgcggt
    12901 cgggctcgcg ggggcgtggc tcttcctgcg cgccgcctcg gtggcgggcc tttccccctg
    12961 gctcgcgccc gcgatcccgc cacgcctcgt cgccgagggg gtcttcctcc ttgccctcgg
    13021 cgtgggactc ggctgcgtgc ccgcgctggt gacgtgctgg gcgaccccgc cgtccgcggc
    13081 tctccgctag tagttgcagg ccgacgacgg ctgggacgtc agagagacgt cgggtccgga
    13141 cgcggtgtac gagaccgcgc acgctccgac gacggtgtag ctcgccgacc ccgagaaagg
    13201 ctgccccgga ccggaccgcg tcccctggta ggttcccccg tagctgtgga cggtcgtccc
    13261 tatctggccg aagaagctga gcttcacggt cgtgtcggtg acggtccccc aggcgcggac
    13321 gttcgagaag ccgctcagcg ccctgttccc gcagacgggg tccttgagga tcgcgcctcc
    13381 cgtctcgacg agggcgacgc ggaagtgctc gctccggtag gtgccgtcgc acctcttcac
    13441 ctgaaggtcg agcgtgtcgt agcgaagtcc ggtgccggag agggtgtacg agccgtcgca
    13501 ggcgttcgtg aagcccgcgg tcgtcgtctt ccctccctgg gtcatcgtga tgctgccgga
    13561 gacggggtac cgggcgcaga cggacgagtc gaagtgggcg gagccggagc cggagtccgc
    13621 cgggttcgac gtgctcgtcg ccgagccgga ggcgtcgccg acgaccttcg acccgtccgc
    13681 gacgtagtcg atcgtccagc tcgcggaccc gctgccgagc gcccggccgt ccttcgtgag
    13741 gccgttcggc gtcatgaccc cggtccacgt cacgcggttt ccggcgacgt tgaagctgct
    13801 cgtcgtgatc gtcgccgatc ccttgtagac cgctccgtcc ttcgccttgt accccgcgcc
    13861 gtagtcgatg acgaggccgt tcccgctctt gcgcgtcgtg gacggcgaga ggttcgcgat
    13921 ctgggtcagc gccgcgtcga tgccgttgag cttcccggtg ttcgcgatgt cggcgatgct
    13981 cgccgtcccg ctcccgaggt tcccgaggcc ggccatggcg ccgttcagga tctcggcgcc
    14041 cggaatcgcg ttcggtccgg acggtatcgt cgtcgcgctc ggggtcggcg tcggggtcgg
    14101 ggtcggcgac gtcgagccgg agacccgtat ggccgggacg aagatcgggt cgccggtgat
    14161 gtggttgtcg atgacggtcg cgaaggcgag gaaccggccc gccggagtgg tcgtccggat
    14221 cacggcgtag ccgtcgtcga gggagctcgc gaaggcgccg aagatctcgg tgatctgccg
    14281 ggactcgtag ggacgcaggt gcgtccggtc gtcctggatc gtgccgagca gcgtgccgtc
    14341 cttcgcgtag aggtcgatcc tcaggtcgat cgtcgacccg gtcgtgttga ggtatccgat
    14401 gttcgtccgg aagtcggcga gagtggacga ggcgcggtgg gtgagctgga tcagccggcc
    14461 ctcctcgccg taggggatgg cgctcgcgtt ctcgtcgagg ccggggacga actgcgagaa
    14521 cgacgcgccg accgggaggc cgacggagtt cgggccgatc tggttgtagg tgcggctggt
    14581 gacgagaagc gtcgcgccgc ccgcgggctc gacccggagc gccgcggcgc cggtgtagcc
    14641 gaagaggctc gagaggacgt cgccgtacct caccgactgc tgcggcccga gactgaagcc
    14701 ccgggacgga ggcgtgccgc cgttgtccga cgcctgcggg agaagcgtga cctggaagcc
    14761 gacggccgtc gagccgggat tgtgcacctc gaggtcggtc cgccagttcg ccccgttcag
    14821 gcccgaggcg tgcgcggagg cggggacgta gaccgtcccg ggcgcggcca cgaggggaac
    14881 gcggccgccc gcctcgaggc ggaacggcgc gacggccgtg atcgcgtctt cggcgggcac
    14941 ctcgctcgcc gcggacgtcc cggtgaagaa gacgatcgag gtccacgttg ctgacgagcc
    15001 gcacgagttg tcggcctcgt tcgactccgc aatcgcgctg gccgagtcgg cccggatcgt
    15061 gagcgtgtgc atcccctccg aaaccgggtc tgtgaagggg ggccaggaga ggagccaccc
    15121 ctcgccgggc tcgatccctt ccgggttcga ccagttcgcg aggccgacca cgacgccgtc
    15181 gatcgagagc gagaactgca tcggcgcggt gaccctggtg gatcccgtgt tcccgacggc
    15241 gaactggatg tgggtggtgt agtaggtcga gaggaagggg gagcagcagg ccgagctggt
    15301 gcagcagccc gactggtagt tcgcgacgac gcaggccggc caggtgctcg gcttgtagac
    15361 gatgaggttc ggcagaccgc ccgagggtgt cggggacggc gtcggcgccg gcgtgcgcgt
    15421 gggcgttgcg gtaggggttc gcgtcggcgt aggcgtcggc gaggtcgatc cggacgtccg
    15481 ccggaccgcg ggtatgaaga ccgggtcgcc ggagacgtgg ttgtcgatga ccgtcgcgaa
    15541 cgcgaagagc gccccgccgg cggtcagcgg cttcacgacg gcgtagccgt cggcgagggt
    15601 gccccaggcg ctgaacgcct ggtcgagctg ccggaagccg taggggggga ggttcgtctc
    15661 cccgccctct cgcttcccga gccaggtccc gtccgcacgg tagaagtcga tcttcacgtc
    15721 gagcgcgctg gcggtcgcgt tcacgatccc gaggttcgtt cggaagccgt ccaggtcgcc
    15781 cgccgggcgc tgcgtcagct ggatcaggcg cccctcctgg ccgaagccga tcgcgccggc
    15841 cgcgtcgagg ccggggacgt actggccgaa cgacgccccc tgcggcagcc cccacgggtt
    15901 ctggccgagg aggttgtagg tccggctcga cacgaggatc gacccggagg cgacgacgaa
    15961 gcggagggcg gcggcgccgt cgaggccgaa ctccgtgagg agcatgtcgg cgaaccttcg
    16021 cgactggcgg gcggcgagcg aataggactt cgaggccggc gcggagttgt tcgcgtcgcg
    16081 ccgcaggagc tggaccgtga agcttgccgt ggtgctcccc gggttgtgga cctcgacgtc
    16141 ggtccgccag ttggagccgt tcgcgccggt cgcgtgcgcg ctcgccggca cgaagacgga
    16201 ggttccggcc gtggcggcga gtcgggccgc gttccccggg accagaggag agtccggcag
    16261 caggcgggtg ccgcccgcga ggagcatgac cgcgagcgtc gatgcggcga gacccagcca
    16321 gggcagcatc tgtccgcggg aggaggatcg gtcgccggcc ttcgtcatgg cccacactcc
    16381 tccgccgggg gcgacgcgac gtgctccagg tgacccaggc gccggagaac cggcagtgcc
    16441 acaggaaatg ctacacccgg gtctctgctc gtgcaccacc ggaagggccg cttcgccggc
    16501 gcgcgaagca gcgccccggc ctcagcccgc cgcggcgggg cgcgacgcga ggaacgcggg
    16561 gagggcctcc ttcgcggcgc cgtagcccgc ctcgaccagc tcctcccagc gcccgtattc
    16621 gttcagccgg aagcggtcga cggacggctc gacgaggagg tccacgaacc ggaggcccga
    16681 ccggagcgcc atgacgctgt tgatctcctg cgtccggagg atcgagccga cgagggaggg
    16741 cgcgcgcgtc ttgcgaacga agggaaggat gcggccccgg aggaccttcc agcccgagag
    16801 ctccgggccg aaccggtacg gcttcacctt gtcgacggcg ggcgcgacgt tgacgccgat
    16861 caccgtgcca cagtcgagcc gggaggccat ccgctccacg gggaagttgt cgacgacgcc
    16921 gccgtcgacg acgacgtcgc cctcctcgat cacgggaggg aagacgccgg gaatcgacat
    16981 gctcttgcgg acggcgcgcc agagcgggcc gctccggtcg acgtcgaccc gcgcccgggt
    17041 caggttcgtc gagacgcaga agaacggcgt ccagaggtcc tcgatgaggc ggtcgccgta
    17101 gagccccttc atcacgcgga ggaccttccc cccggacatc agcgagacga gggggagcgt
    17161 ccggtcgaag atcttccccg gcgacgcgaa cgtctccgcg agcgcgagga gcggggcgga
    17221 ggcggcgtgc agcgcgaagc tcgcgccgac gacggccccc atgctcgtcc cggcgacgag
    17281 gtcgacgtcg acccccgcct cctcgagcgc ccgaatgacg ccgatgtgcg ccaccccgcg
    17341 cgcgccgccg ccgccgagga cgagcgcgac gccgcgcccc gcgaggcgcc gcgcgaggcg
    17401 gacctcgtcg cgggcgttcc cgggccggac gtgatggtgg tccgcgaggt cgcgcccggc
    17461 gagccaggcg gccgtcccgc gcggctccgt cgtctcgggg gcgtggatca gcacgagctc
    17521 cttccgcgcc gggagggcca tccccgcgag ccgggcgtcg atcgccccgg gggccgcgtc
    17581 ggcggcggcg tcggcaacga ggaggacgcg gtccgcgcgc cggaggcagc gctcccgcca
    17641 cgcctcgccg ccggaccccg cgtcgaggac gaggtggcgc gtccggtcct cgagctcgcg
    17701 gagagagacg tcgacggcca ggccgagcgg cccggcgagc ggcgcctccg cgacgcccgg
    17761 ccgcccgtgg cgcgcgtcga ggccggcggc gtcgaggagg agcgtcgagc cgagcgcctc
    17821 gaggcgggac gcgagcgtcc gcgcaagcgc ggacgcggaa gccgacggcc cgagcggcag
    17881 gaccgcgatc gtcaggccgt ccggcgccgg gacgggggag gcggtcccgc tcttcggccc
    17941 cgacgagccc gcctggcgcc cggcgacgat cgacgcgacg cgcagcagcg cctccgggcg
    18001 cttcagggcg aagccgcgga acgcgtcccg tccgagccgg aggacctctc cgtcgcggag
    18061 ggcgacgacg gatgcggagc gcggctggtc cgcgaggagc ccgagctcgc cgacggtgct
    18121 gaacgcgccc gtctcccgca cgaccgtctc ggtgccgtcg gcgccgcgga ccgtcgtccg
    18181 gaaccgcccg gtgacgacga cgtacatcgc gtcgctcggg tcgccctgtc gaacgaggag
    18241 ctcgcccgcg gcgacgcgac gccactcgac ggcctcctcg aggtcgcgaa aggcgtccgg
    18301 ctcggtgatg ccgaagagct cctcccagac gcgcaggagc tgcgcgcgac gcaggcgcgg
    18361 gccgatcgcc tccgtcagcc tggaagcgac ggcggggtag cgacgggcga gctcgtccca
    18421 gccttcgcgc gggagccgca cgaggacgct ctcctcgatc gccgtcagcg tcgccgagcg
    18481 gggctggccc gtcaggatcg ccatctcgcc cgtccactcc ccgggtccga tctcgccgag
    18541 cgtcacctcg gcgccggacg gagtggtgcc ggtgacgcga agacggccgc cggtgacgac
    18601 gaacatcgcg tcgcccggct ctccccgccg gaagaccgtt tccccgcggg ggactcggac
    18661 cgtcgagacg agggcgtcga cggcggcgcg gtcttccgcg ggcagggcgt cgtactcggg
    18721 gaagctgaag agcgaggcgc cgtctccggg ccgcgcggct cgttcgtgca tcgaagtccc
    18781 tcctggagac ggtctttgaa tggagtactt cggatcctaa ccgatgagac cgaatctcca
    18841 gggtccgcgt ttacgcgcca gctcgaaggc cggtccgggg gcggaaggcc ggcgccctct
    18901 ccgccggtgg tacgaggccg gccggcgccc tacggcgtca tcagcgtgca gggctccgtg
    18961 ggctgaccgt tcgcatcgaa gcagaggagg gtcccggccg gaagctcctt ctcgccctgc
    19021 ttgaagacct cgccgagggt gcatttcttc agcctgccgt tctcgtggaa gtagttctcg
    19081 ctccccttgc acggatagcc ctggatcgtc cgcgggcctc cgaagagctc gagccggttc
    19141 agcgccccgc cggggtagag ggtgacccag tccccctcgc gggcctcgac gtcgccgaac
    19201 gtcttcgccg ccgcgacgac gccgcgcctg aacctgccgt cctcgtagag ggcgagggat
    19261 tccttcacga ccagcccgtc gaacgtcgcc gtcccggcga tcttgcactc cttgagcttg
    19321 ccgtccgggt ggaggacgac gctccagttc atccggcagg tgtagggccc gtagacccag
    19381 tccgcggcga ggaggcactc cttgagcttc ccgtcggggt gatcccgacg agagccgtcc
    19441 ttgcaggaag gatcggcggg ggccggggct gcagccgccg cggcgggggg cggatcggcc
    19501 gcgggggcct cggccttccg gcaggcgagc gggcccagga cgagaatcgc gatcgtggcg
    19561 gcggagacgg tggcgtcacg tctcgacatc ggggactcct cctttcgtgc aggcgttgcc
    19621 cgcgcaccgg cgtcccggac ggggtacccg ggctggttcc gcgagttgag gctcctactc
    19681 cttcagcgag cccgtgattg cagcgatcgc cgcttcggcc tcgttcgcga atccgggcgc
    19741 gaagacgaac gcgaggacga ccttgtcgcc ccccttcttc gtcgcgacgt agacctgggc
    19801 gagcgacggc gacgggatct tcttctcgag cgccagtccc ttcaccttcc tcccgaggag
    19861 cgtccgctcc acgggcctgc cggcggccga cgtcgcgagg aatgtcgtct tcacgtactc
    19921 gaggagctcc gcgtcgctca tcccggagtc tttcgggaag gcgacggcga ggaggctcag
    19981 cttctcgcct ccggccttcg cgtcgggcgg gtgaacgacg cggacggcgt cgaggccggc
    20041 gctctccggc agggagaagg agaccggggg agtcgcctcg aggccatgca gctcgatcgg
    20101 cgcgggcgag gcggctccgg ccgtgagcgt cccggcggcg aaggcggcga gggtcgcgac
    20161 gagcacgggc gcgaatcggt tcggcatctg ttccctcttc gtgcgatcgg tcgttcggcg
    20221 cggcgtcact tcaccaccgt cgcgacgaac gagcccttga acggcccgtc ctggccgacg
    20281 acgtcgaacg tcccgcagac gcgcttgtcg gtcacctcca cgagcgtggc cttgccggac
    20341 ttcaggccga gaccgtagac gacgggcgtc ccgtcgtcct tgccgaagcc gaggtccccg
    20401 cggacgttca tcgggtccca gacattcgcc cgcatcgcgt actcgcccgc gacgacgggc
    20461 ttcgacgcgt tcatgaacgt gatcgcgagg gagacctggc ccttcccctt cacgggggac
    20521 gtgtcgaggc gcgccgcggc cgccttcacg tcgaagtcgg cgttcgcgat gaagacggtg
    20581 atgccgaagg cgctttttgt cgccttcgtc ttcgtcaggg ccttcaggtc gaacggggcg
    20641 acgggtttgt aggccgcgtt cttcgactgc ttctcgacgg ccgtcgccct cgggcagtcg
    20701 gcagcggacc ctgagagggg ggcgagcgcg aggacgaggg cgagggggac gaacgtggct
    20761 ttcatgggag cctcctttgc ggggcggagt ccgccccgcc tcttttcccg gccggaacgc
    20821 tcctccgggt gcctactctt cccggcactc gagcgcgtcg cgctccagga ggccggccgt
    20881 tgtctgcttc ttgccgacgt acctcacgag gagccacccc ggcgtcccct cgttcacgag
    20941 gccgacggcc tcgacgacgt tccccttcac gacgtatccc ttccgcggct cgggcgaggg
    21001 cgcggcggtg aggaagcgcg ccttcggcgc gacgaccgtg cagttgaagg agggcttcca
    21061 gccctccttg gcgaacgtgt cgcccggcca gttcgcgccg cagaaggagg cgatcccctt
    21121 cgtcgcctcc atcgtcacct ttccgccctt cgtcgtgaac gcgaccgtcc cctgctcgtc
    21181 gacgtactcc cacctcccgt cgccgacgaa gccggcgacc ccgatcccgt cgcacatgtg
    21241 cgccgtggcg ccgaagacgc cggagtagga gaaggtgagc gtgtcgccgg ccctcatcag
    21301 ggcgagcggt ccgtgctggc cgaaccagac gccggtcggg tcgaagccgc ggtcgtcggg
    21361 cgtctgggcg ccggcgggac cggccgcgag gaacgcgagg aggagcaggg cgcacgaggg
    21421 cttcatcgag gcctccttcg gtgtcgtctc accagagcag gaagtcgtag gtctctccgg
    21481 cgtcgaggtg gtaggtcccc tcgatccgcc agctgccgtc ggcgtcgttc accttcgcgt
    21541 actccctctc gacgcggccg gggtaggtca gctcgtagag gaggtggagc tcgcgcgtct
    21601 tcgagaagag gttgcgggtg aggtccgcgc ggaggaggcg gcagccgcgg agcgcacccg
    21661 tctccgtgcg ccgcttctcg tgggccgcga cgaacttctc ccgcggcacc tcgtcccggt
    21721 agctcgcggt gaggcacttc tcccacgcct cggcgaagcg tccctccccg acgaggcggc
    21781 cgtagtcggc catcacgccc cggtcgatct ccgcggcgac atcccagtcc tttccctggc
    21841 cgcggatcgc cgcccagacg acccctccga tgaccgccac gatcgcgagg gcgaatacga
    21901 gtcccatggc gggagtcgtc ttcacgtgtc ctcccatccg atcgggttcg cccgctcgcc
    21961 gtgacgacgg cggcgttcgc gcccgcgcct acccgaccga attcgcatcg acgagattac
    22021 tccccttccg cgcgaattgg acaacggcag aatccggacg ccggggggcc ctgttccctc
    22081 cgcgccgtcg atcgcgggtg taacgtcgac agatcgtcgg ccggatccga aggaggtccc
    22141 gcttgaggac agccccgttc gccctcctcc tcgcccccat cgcactcgcc ggggccgccg
    22201 cggtggaagg agagcattca tgagcgcagt cgcgcccgga ggcgggggat cgggggccgg
    22261 tgacagcgtc cgcgagcgga cacccatccg gaccgtcgcc tacgtgatct ccgccgtcgg
    22321 ggcgctcgtg gggcccgtct cgttcctcgc cttccccggc ttcggtgcct gggggagcga
    22381 ggacctggag ggagccggcg tcctcgctct cacggcgctc ctcatggcga gcccgttcgc
    22441 ggcgttcttc ctcgcggccc gcctctcgag gacgaaggcc ggcgcggccg tcgtcgccgc
    22501 cctggccctc gtggccgtcc tcttcggggc gctcttctac gtcgacgcgg cccttcggag
    22561 ggacttcgtc ctcctcctcg cgtcggtcgg ggtccccgcg gtccagtggc tcatggcgct
    22621 gccggccgtc gtcgcggcgc tcgtcc
//
LOCUS       DAOTEZ010000011        22144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_1544_length_22144_cov_6.381366, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000011 DAOTEZ010000000
VERSION     DAOTEZ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 22144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22144
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1544_length_22144_cov_6.381366"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="P5164_01145"
     CDS             complement(<1..436)
                     /locus_tag="P5164_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HRY42524.1"
                     /translation="MSPRQARAIEVAGARTHNLKGVSCRVPIGAITVVTGPSGSGKSS
                     LAFDTLYAEGQRRFVESMSTYARQFLERMERPDVERIDHLLPAIALEQKAAARSARST
                     VGTATEIHDVLRLLFAAAGTVVCPDCGLEVRRDSPAGVADALV"
     gene            complement(433..1044)
                     /locus_tag="P5164_01150"
     CDS             complement(433..1044)
                     /locus_tag="P5164_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRY42525.1"
                     /translation="MTRTDETPAGGRRLAPYFGLLTRSVQRARLAAALPHVPPGARLL
                     DLGCGLTDLPARFPSYVGCDRNPVVLDEMRRLHPSAAFASWDVESDEPPPEVRAGAPY
                     DAILMLAILEHLAEPARALGRAGALLAREGRLLATTPHPLGRLPLEAGARLGLLSRHA
                     DEEHETLLDRAGLERCAAGAGLAVVSYSRFLLGLNQLVVLARR"
     gene            1112..1750
                     /locus_tag="P5164_01155"
     CDS             1112..1750
                     /locus_tag="P5164_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRY42526.1"
                     /translation="MNEPVAPFPFAVLAFSSLFSVVNPISGAPVFVSLTAHLNHGERR
                     RAAVRAVLTGLATLAVFSAAGGAIFSFFGITVPAFQITGGILFALMSLRALEHGHEEV
                     PETSAPGRDPSIVPVGIPLIAGPGAISTVMVLVGQAGGASHRLALGGAIVATLAVTLA
                     ILLAAPAITRRLGDTGMKITNKVMALITAVIGIQFVINGTTAVAVQILRAAR"
     gene            1760..2272
                     /locus_tag="P5164_01160"
     CDS             1760..2272
                     /locus_tag="P5164_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY42527.1"
                     /translation="MNGTDISIRPARREDVPVVARLIRELADYERLAHECFADASALE
                     EHLFGTRPYVEVILAEVDGTPAGFALFFHSYSTFLTKPGLYLEDLYVVPERRGLGLGR
                     RLLAELARLAVERGCGRLEWSVLKWNAPAIGFYERLGAVPMSEWQVYRLTGEPLRAVA
                     GMAARPAGGA"
     gene            2269..4014
                     /locus_tag="P5164_01165"
     CDS             2269..4014
                     /locus_tag="P5164_01165"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HRY42528.1"
                     /translation="MKERRRRPGYDTVALPKPGAEKPALEALAFAALPDPAAVLDGEG
                     RLLAANAAARAEGAPAPEGTGAPLGQLLPFWNDPESRDALLAAASGSARATGVEARVA
                     FESGSRRKAFRVSARHLPGDGPSRVVAVARELASPATGRIELPTARPPEGTSDAIDPV
                     TGFVTRTAFQALLETAIEESARAKQALALLFFDLDDFKALNDTHGLAAGDEYLRRLGE
                     ALLVDLQPGRVFARLGGDEFGLIAPQTNAAEAALEADGLVRFLTEFSPTFEGRPLQLT
                     ASVGVALHPAHGQRASDLMLAADLAMHQAKARGRARFILHDPAARERERIGLLRGQAD
                     RIRTALAGARFVPVFQPIAEVATGRVVAAETLVRLREEDGTLVRPDQFLDAAERFGLV
                     TAIDRVVIAGVFDALVASRRRISPDLEVSVNLSGLDFEDDALVAEISRLARLKGVRPS
                     RITFEITETAALRDLGRVQDFTNALTSEGFRFALDDFGVGFSSFRYLRDLPMSTLKFD
                     QSYVRDLPHKMENRVFVRGIAEICRGFGVKTVAEGVETPEILEILRELGVDRAQGYHV
                     GHPSLELPGRSSEAT"
     gene            complement(3960..4724)
                     /locus_tag="P5164_01170"
     CDS             complement(3960..4724)
                     /locus_tag="P5164_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42529.1"
                     /translation="MRLARRVLEDPEAIEERRGPRRGRVEELEGVASPLGRDSRGVER
                     GGGGREKLGQALEAAGEAPAGAPPEGAAGLRARGEGPSEGRGEAPSPPGDVLPEPRDL
                     RGVDAERGELVREDRPKEALVLDPLREERAADLLGTERREAPANVPRRDPVRVAVLRF
                     RKAAKEAGRRLEAPQRDPDVVNEGRVASSAGALLEPAEPLRLARERVFQEGEDRAGSS
                     VRQRGPPPPILGPRCEGAPQVASLERPGSSSDGWPT"
     gene            4928..6769
                     /locus_tag="P5164_01175"
     CDS             4928..6769
                     /locus_tag="P5164_01175"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRY42530.1"
                     /translation="MSRLREAARLAAALVPLLAVAATPAPPPEPPRRIVVVSFDGAGG
                     LDFVRHREALSPDGFLRAEHEGVSAERLQTVTPSLTAVAHASISTGALPQATGIVSNT
                     YWPAGGPLKARADGFEAEPAVETLWEALARQGHRVAALSWPGVSGASTRSSTPVGLRW
                     APLRSRGFVWRGPEEGAVLPDAAIALPPEVRSYSPPRLVRIEPPRGRDGASPRPLLFV
                     VVDTRDDGRREYDGLAALDEAGELEGRLRPGDWLALSEKTDGVARRGRWIKLLRLAPD
                     ASRVELYVGGIGQTEAWPDDFQRTLDRRCGFWPGPPDEALLSGPEPDVRSFLEMAARF
                     TEFFTAAYEVAERRGDWDVLLGYQPLLDEVGHSLMPPDPGTAGFDAAGAERAEAAMTE
                     AWRIADRAAARYLRFLELGGDVLFLSDHGQRPVRRVVHPAELLRRQGWITTDSLAGRP
                     VVRPDSPADVAVSGGTALVVLNRAGEMPGGVLSPDEGAGVLADIASYLRDLKDETGAP
                     LFETVALRGEAATLGLDHPNVGDLLLLAGKGTALRGGFPKLGAEAPVLLPADVAGQHG
                     YGPDPALDGIFFHVGRGLEPARLPLVRAVDVAARVAARLGLSPPGAR"
     gene            6766..7425
                     /locus_tag="P5164_01180"
     CDS             6766..7425
                     /locus_tag="P5164_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HRY42531.1"
                     /translation="MTPPVVSVPLLSSSARRGEAQRHLERHPLGGLRGEAPGEDATRV
                     GLLGGLRHLHVFFEVRSEPPLRVRCRRLHENVWEDECVELFWADAAEPSRYLEIVVNP
                     EGVHYTARVANPHASRETWNVIRGVPVPGLRVRVEGTPADEPSTRWTSWRCGISVPWA
                     VLTPPFGRPRPGDERRGNLTRIARGKTLRYETLAPTGRATPPDFHVPSAFATFRFVEI
                     L"
     gene            7437..8033
                     /locus_tag="P5164_01185"
     CDS             7437..8033
                     /locus_tag="P5164_01185"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRY42532.1"
                     /translation="MERPSGPVVAVATTAGAPADLELVERIRQGDQAALDLLYRRYSA
                     PVYSLVWKVLQSSEEAEDVALDVFWQVWRQAGRYDPSRGAPPAWIFTLARSRAIDRLR
                     ARKRREDRTVSIDDPELNIDPLDEKAAPDEVVSFRQNRDAVRAAMSKLSAVQREAVEL
                     AFLQGMTHVEIAEKLGQPLGTVKTRIRQGLIRLRKHLD"
     gene            8041..9180
                     /locus_tag="P5164_01190"
     CDS             8041..9180
                     /locus_tag="P5164_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRY42533.1"
                     /translation="MRPTHGERPGPLPAALRPALRRWFLKNRRDLPFRRTRDPWAIWL
                     SETVLQQTTVAAGAPRFEGLLALFPTVEALASAGERELLAAWSGLGYYARARNLHRAA
                     RLVAELGGGVPRTVEELRALPGVGPYTAAAVASLAFGAPVPLVDGNVSRVLSRVGAIP
                     GDGRTGASGAAVARAAEAFLDRRRPAEHNESLMELGALVCLPRAPLCPGCPLRPACRA
                     HALGRPEAFPAPRARKAAVRLRLAAGVARRAGRLVLVDDAHLVRGHLVVPLLELAAGA
                     DAALRLRAAWPSLAGRRAKRLSPLGTVRHAILERRYVVEVFAVEEAGPTDERVPVRPV
                     HPRDLESEPRGGLLGKVLALSASAGSPAPPGRARPARAPRKPTGG"
     gene            complement(9101..9673)
                     /locus_tag="P5164_01195"
     CDS             complement(9101..9673)
                     /locus_tag="P5164_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HRY42534.1"
                     /translation="MPILKPEPDLFPEELFSLPAERFPWWVVHVKSRQEKLLARECRR
                     RGIPFYLPLCERRSPRDRRGRVAYVPLFTGYLFARGDLEHERLELLKTNLCLQVLAVP
                     DQEGLAADLGQVRRLQEMGVPLVPWPDLTKGSVVRIAEGPFLGMTGRVTKVKGKSRFI
                     VSVRFIHRSVSVELERDALVPAEPESPRKR"
     gene            9717..11537
                     /locus_tag="P5164_01200"
     CDS             9717..11537
                     /locus_tag="P5164_01200"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRY42535.1"
                     /translation="MAKRTGDATTGGRELLAPLGTLVALSIPFFVLTLASAEATRDAK
                     LAAQAAGGVLVLLGLAYGRPSAGREPAGRGARLALGALVAFLALFAASAAAGLLRGRD
                     PLDLLPLLPALALFAWGATRPGEESAGRALALLVGCGALTGLLAALQRFAGLFRLPVE
                     APEPRFFATALIGNPGDVGASLVVPALLAAASLVRGERRLAAALALAAMLAGLAAAAT
                     LAPLAAFGAGAAILVLGDLRRRLLPALGAALLLLGLLQAGGVLSRVSEKLSSGAIDEL
                     TTQRDIGVLAALETIRAHPFLGAGPGGFSADFARARLAAEERFGRRLVHRSASAHFDN
                     AHSDPLTVAAETGLPAAAALALALGALLVPLAAAARRERSPGRPEGLPAEALLASLAA
                     VLVLSLANFPIQIVPVSGPFALLAGLALARVGGSFPLPSGRAGRTALALLALLLASGA
                     AVRLSGGLALARVENALRLAPGTSGATRGELVASALVEARRAVALRPRSATAHLALGS
                     ARAAAADLPGAVASMERSRELEERAETLLNLGRLAIARGDDRAARPFFVRGVWLFPRL
                     AAAIPPAGDPDGVVAEVARLEAALPSGGAPPPPPPPLRLR"
     assembly_gap    11620..11719
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11720..12708)
                     /locus_tag="P5164_01205"
     CDS             complement(<11720..12708)
                     /locus_tag="P5164_01205"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRY42536.1"
                     /translation="MRHASPARRALLAAGDLLGAVAAFAAVVWLRRVVALPGTDGVLP
                     AEKVPLDPWPWLVVVGLSALLAHSLAATWTEPLETLRERGGLFVAAVLTALFLVGAFF
                     LLSRALPRTVALLYVPAAWALHALFRSVVDRAAPPPVRRVLVLGDGEDARRAAAALEA
                     GEAPGYALVGWEPDAVGAIAVSREPGAPRQADHVVLASGNPREREAFAALVERSAAGG
                     FELWLLPGLADVVASHVATRSLGDLPLTRVAARGASAPAFALRRLLDVVLGFPLAALA
                     AVPVAVVSLVVVLETPGPAWIRQRRVGRGGRELHVWKIRTMRADAERETGPVLA"
     gene            complement(12705..13859)
                     /locus_tag="P5164_01210"
     CDS             complement(12705..13859)
                     /locus_tag="P5164_01210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY42537.1"
                     /translation="MRALLVVPRLPGTGFTGDRVRAELHLEALAELGARVTIVGGVPP
                     NRPVAAVAGAEEVRGVPIRVAALPFHLTRAFVRGWPLQTALFDGAWERALAGCGRFDL
                     VVLLLARLQPRLRTLLPRAPLVVDYVDALSEAARQNAERDPALWRRLYWRAEGARLMR
                     AEREAARGARLLLATTPADAAALPEGTEAIAIGARIGPPPPRERAQVVVFTGRMGYRP
                     NAVAAELLLREIWPRVRGRVPGAELVVGGADAPASLRRLAEGTPAARLVSPVSDMRAL
                     LLSARVAAVPVDMGTGTPNKVYEALEAGCAVVATPAAAARAVLDGVAAPLRTAADPEA
                     FAREVSDLLADAESAEALGAAGRAFAVAHADRGEIARRLARLLSRAAEGA"
     gene            14079..15728
                     /locus_tag="P5164_01215"
     CDS             14079..15728
                     /locus_tag="P5164_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42538.1"
                     /translation="MGSQDSAAEARRPRRELTLLPSLGAFLVSAGLFLAHAAFLFPLH
                     LDDPYITFRYGRNLARGDGLVWNVGEAPVEGFTSHAWVVLAALAEASGSPPLLVAKLA
                     GLAAGLLVLAVLAFGTRRLLPNASDRLAAALLLATSTSFVSMSASGMENVAFAAAASL
                     LIPLLGPWDAPDAAGQVTPRRAALAGLLSGLLVTIRPAGHVFVVVLLGALAFAARATR
                     ERRPLVAAGVAAGAVLVPFHAFRLATFGSLVPLTYLAKHTGEPFLERVASGLRWLGER
                     GGPLLPIFFLALLSALVLLRGRAARASAALLAVFPAYAVAVGGDSSAFPGARLLLTVL
                     PFAAALAVAAVRRVTGGGLRAAAFSGLLLASFTLAQVPDMTSLRRGLALDRRSGAAGG
                     PARAAFDHLAELLDPRPLRLSSYLVVATPPGERIAVPWAGRVPYETGLATVDMLGLND
                     AHIARLPKLDRGVDVKYDPDYVLSKRPWYVCENFVLGGVPVTALARLSDDQLARLGAF
                     RAGQRALLRSPLLARDYDVDAGIPNSAGGSITCFRRREPAR"
     gene            complement(15694..17226)
                     /locus_tag="P5164_01220"
     CDS             complement(15694..17226)
                     /locus_tag="P5164_01220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY42539.1"
                     /translation="MEGDEGARTTSEDAEERTLRRAFVFAILVGAGARLFLAFARPLW
                     ADEVFTLTLARRPLADLLAALRLDSGPPFHYLAARLLLLPFPAPGAADVVIRLLSVAA
                     SLLHVPLMLRIGRRCGAARTGLVAAALFLLFPLAVASGSEGRGYALASLLALATFERI
                     LELEEAPRPRTALAAGLLGGAAVLTHYLAALPVAGVLVSAWARSRRRRLVALAGVVAA
                     ATAAAWLPVALGQPRDSMAWSEARPLGERALQSAANLGLGLPVEPGPARYAGPLALVV
                     LGLAIVGGRGARLPAAAPLLIGLGLLLPLLAFSGSALLPDRTALAFLPLAAFVLARAR
                     WGEVALASGAASAVVLALSLPAWVRPTPASELAAALGPKVRGGARVVAAELWGPELDY
                     RLAREGLAGRVTLFPSDVALHPGWYSDAAPSEERLRAEASQAVDGAGGGPSLYVFSPA
                     TRAGRALLASLGPVGARRIAAAGVFEIWLGPAGGPSAGRAAPPPRGEPPLSAPAPSVG
                     SR"
     gene            17533..18057
                     /locus_tag="P5164_01225"
     CDS             17533..18057
                     /locus_tag="P5164_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRY42540.1"
                     /translation="MQVQVSSPPPPPATACACGEATEAEKLARIAEVVEEFRDKPGSL
                     IQILHLAQGIYGYLPIHVQRLVAEKLDIPVSEVWGVTTFYSFFSTKPRGEYSIRVCLG
                     TACYVRGGVEIVKRLSQTLGVGVGETTPDGKFTLEVMRCIGACGLAPALLVNDKVVRQ
                     VNPDKLNRVLALCE"
     gene            18072..21176
                     /locus_tag="P5164_01230"
     CDS             18072..21176
                     /locus_tag="P5164_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRY42541.1"
                     /translation="MTPEPNLTRPASKADLDAIAARWHAEQQGKKHQLLVCAGAGCVS
                     SGCHAVKHALLAALKDAGLEKETAVHETGCLGSCHLGPTLVVQPDGVLYTELKPENMK
                     GIVRQHLVHREVVEQHCWTDPETKEPVPHLSDIEFFKRQVKIATARCGSMPYASIEAY
                     IASDGYQGLWKALSTMTGEQVIQEMRASGLRGRGGGGFPVGAKWQAGFKAKGAEKVVV
                     CNADEGDPGAFMDRSLLEGDPHSVLEGMLLAGYAIGASQGYVYVRAEYPIAVERLEAA
                     IRQARAWGFLGPNVMGTKFSFDVEIRIGAGAFVCGEETALMHSVEGKRGEPRQKPPFP
                     FERGIFGKPTIINNVETLANVAAIVKNGGAWYASLGTSKSTGTKVFALAGKVENTGIV
                     EVPMGITLGEVVHDIAGGIPKGKRFKAAQTGGPSGGCLTREHLNTPIDYDSLVHLGTI
                     MGSGGLIVMDEDSCMVDVARFFMDFVQDESCGKCVPCRIGTKRMLEILTRITKGQGKD
                     GDIELLEELAANCKETSTCGLGQTAGNPVLSTLRLFREEYEEHIRDRHCRAGVCADLF
                     KSPCQNACPAGVDVPGYVALIAAGRPRDAYHLVRRENPFPAICGRVCTHPCEDKCRRA
                     QLDEPVAICDLKRYAADYVFANDEPHRDLVFPKKAESVGVVGAGPSGLTCAWYLARLG
                     YGVTVYESLPVAGGMLASGIPEYRLPKEVLAKEIRLVEQVGVRILTGVEVGKDVTFEE
                     LQKQHQAIYVATGTHFPNKAEIPGEEKAGIAHGVSFLREANLGRKVEVGKNVVVIGGG
                     STAFDAARTVLRLGAEKVTVLYRRQVEDMPADPREIREAREEGIEILPLVAPVEFLGN
                     GRVEGVKCVKMELKGFDDAGRRKPKTVAGSEFVVPADMVIPAISQHSDLPFIDRDEVQ
                     LTKWGTLVVDKETMMTRKKGVFSGGDVVRGPDVAIQAIADGKRAARAIDRYLGGTGVL
                     NKGEEIPIPRPTDETDVAEHERFPMRFLEPDSRRKGFQEVAAGFHKLNAIAEAMRCLR
                     CDRR"
     gene            21187..>22144
                     /locus_tag="P5164_01235"
     CDS             21187..>22144
                     /locus_tag="P5164_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HRY42542.1"
                     /translation="MTNPVHLVINGRDVRAESDWSVLEAAKHNGVKIPHFCYLEGVHQ
                     IGSCRICVVEVEGMRSLQASCTVPVREGMVVKTNTARVKKAREVLYELMLSDHPRNCL
                     SCYRNQNCEFQKLGEEIKIPDCRFEGERSKTFVDDSSPSVYRDTSKCILCRRCVTVCS
                     EIQGVGVLGVNNRGFGTTIGPAADLPLGTANCANCGQCVMVCPVGALLTRADMTPVWN
                     ALFDPKVRTVVQTAPAVRVALGEMFGMEPGTIVTGKMAHALRLLGFDDVFDTNFAADL
                     TILEEGTEFLHRALAAVRGQDVVLPMITSCSPGWIKYVEHEFP"
BASE COUNT         2790 a         8670 c         7750 g         2834 t
ORIGIN      
        1 cgacgagcgc gtccgccacg cccgccggag agtcgcgccg cacctcgagg ccgcagtcgg
       61 ggcagacgac ggtccccgcg gcggcgaaga ggagccggag gacgtcgtgg atctccgtcg
      121 cggttccgac cgtggagcgg gccgagcggg ccgccgcctt ctgctccagc gcgatcgccg
      181 ggaggaggtg gtcgatccgc tcgacgtcgg ggcgctccat gcgctccagg aactgccgcg
      241 cgtaggtcga catcgactcg acgaagcggc gctgcccctc cgcgtagagc gtgtcgaagg
      301 cgagcgacga cttgccggag cccgacggcc cggtgacgac cgtgatcgcc ccgatcggga
      361 cgcggcagga gacccccttg aggttgtgcg tccgcgcgcc ggccacctcg atggcgcgcg
      421 cctgtctcgg gctcatcggc gggccaggac gacgagctgg ttcaggccga ggaggaaccg
      481 ggagtacgag acgacggcga ggcccgcccc cgcggcgcac cgctcgaggc cggcgcggtc
      541 gagcagcgtc tcgtgctcct cgtcggcgtg ccgggagagg aggccgaggc gagccccggc
      601 ctcgagcggg aggcgtccca gcgggtgcgg cgtcgtcgcg aggaggcgcc cctcgcgcgc
      661 cagcagggcc ccggctcggc ccagggcgcg cgcgggctcc gcgaggtgct cgaggatcgc
      721 gagcatcagg atggcgtcgt acggcgcccc ggcgcgcacc tcgggcggcg gctcgtcgga
      781 ctcgacgtcc caggaggcga aggcggccga tgggtggagg cggcgcatct cgtcgaggac
      841 gacggggttc cggtcacacc ccacgtacga cgggaagcgg gcgggcagat cggtcaggcc
      901 gcagccgagg tcgaggagcc tcgctccggg agggacgtgc ggaagcgccg ccgcgagccg
      961 cgcgcgctgc acgctcctcg tgaggaggcc gaagtagggg gccagccggc ggccgcccgc
     1021 gggggtttca tcggtccgtg tcatcggaga gcgtcccgcg acggtcgccc cggggcgacc
     1081 cgtatgatag ccgtccggcc cggccggaac gatgaacgag cccgtcgccc cgttcccctt
     1141 cgcggtcctc gcgttctcct cgctcttctc cgtcgtgaac ccgatctcgg gcgcgcccgt
     1201 cttcgtgtcg ctgacggctc acctgaacca cggagaaagg cggcgtgcgg ccgtccgcgc
     1261 ggtcctgacc ggcctcgcga ccctcgcggt cttcagcgcc gcgggcggag cgatcttctc
     1321 gttcttcggg atcacggtcc ccgcgttcca gatcacgggc gggatcctct tcgcgctcat
     1381 gtcgctccgg gccctcgagc acggccacga ggaggtcccc gagacgtccg ccccgggacg
     1441 cgacccgtcg atcgtcccgg tcggcatccc gctcatcgcc gggcccggcg cgatctcgac
     1501 cgtgatggtc ctcgtcgggc aggcgggggg cgcgagccac cggctcgccc tcggcggcgc
     1561 gatcgtcgcg acgctcgccg tcaccctggc gatcctcctc gccgcgccgg cgatcacccg
     1621 ccgcctcggc gatacgggga tgaagatcac gaacaaggtg atggcgctga tcacggccgt
     1681 catcgggatc cagttcgtca tcaacggcac gacggcggtg gccgtccaga tcctgcgcgc
     1741 cgcgcgataa tccccgcccg tgaacgggac cgacatctcg attcgccccg cccggcgcga
     1801 ggacgtcccc gtcgtcgccc ggctcatccg cgagctcgcc gactacgagc gactcgcgca
     1861 cgagtgcttc gctgacgcct ccgccctcga ggagcacctc ttcgggacgc ggccgtacgt
     1921 cgaggtgatc ctcgccgagg tcgacgggac gcccgcgggg ttcgccctct tcttccacag
     1981 ctactcgacg ttcctgacga agccgggcct gtacctcgag gacctgtacg tcgtcccgga
     2041 gcggcgcggg ctgggactcg gccgccggct cctcgccgag ctcgcccgcc tcgccgtcga
     2101 gcggggctgc ggccggctcg agtggagcgt cctgaagtgg aatgcgcccg ccatcggctt
     2161 ctacgagcgg ctcggggccg tcccgatgtc ggagtggcag gtctaccggc tcacgggaga
     2221 gccccttcgc gcggtcgccg gaatggcggc ccgcccggcg ggcggcgcgt gaaggagcgg
     2281 cgccggcggc ccggctacga caccgtcgcc ctcccgaagc cgggagccga gaagcccgcg
     2341 ctcgaggcgc tcgcgttcgc cgccctcccc gaccccgcag ccgtcctcga cggagagggc
     2401 cggctcctcg ccgcgaacgc cgcggcccgg gcggaagggg cacccgctcc ggaagggacg
     2461 ggcgctccgc tcggccagct cctccccttc tggaacgacc ccgagtcccg cgacgccctc
     2521 ctcgcggcgg cgtccggctc cgcgcgcgcg acgggcgtcg aggcccgggt cgccttcgag
     2581 agcggctcgc gccggaaggc cttccgggtc tcggcccgcc accttcccgg cgatggtccg
     2641 tcccgggtcg tcgccgtcgc ccgggagctc gcctcgcctg cgacggggag gatcgagctc
     2701 ccgacggcgc ggccgcccga agggacgtcc gacgcgatcg acccggtcac cggtttcgtg
     2761 acccggacgg cgttccaggc gctcctcgag acggcgatcg aggaatccgc ccgcgcgaag
     2821 caggcgctcg cgctcctgtt cttcgacctc gacgacttca aggcgctgaa cgacacgcac
     2881 ggcctcgcgg ccggcgacga gtacctgcga cgcctcggcg aggccctcct cgtcgacctg
     2941 cagccggggc gggtcttcgc ccggctcggc ggcgacgagt tcggcctgat cgccccgcag
     3001 acgaacgccg ccgaagcggc cctcgaggcg gacggcctcg tccggttcct gacggagttc
     3061 tcgccgacct tcgaggggag gccgctccag ctgacggcga gcgtcggcgt cgcgctccac
     3121 ccggcccacg gccagagggc ctccgacctg atgctcgcgg ccgacctcgc gatgcaccag
     3181 gcgaaggcgc ggggacgggc gcgcttcatc cttcacgatc cggccgcgcg ggagcgggag
     3241 cggatcggcc tcctccgcgg ccaggccgac cggatccgga ccgcgctcgc aggcgcccgc
     3301 ttcgtgccgg tcttccagcc gatcgcggag gtcgccacgg gccgcgtcgt cgcggccgag
     3361 acgctcgtcc gcctccggga ggaggacggc acgctcgtcc gccccgacca gttcctcgac
     3421 gcggccgagc gcttcggcct cgtcaccgcg atcgaccgcg tcgtcatcgc gggcgtcttc
     3481 gacgcgctcg tcgcgtctcg caggcggatc tcccccgacc tcgaggtttc cgtgaacctc
     3541 tcgggcctcg acttcgagga cgacgccctc gtcgccgaga tctcgcgcct ggcgcggctg
     3601 aagggggtcc gcccctcgcg gatcacgttc gagatcaccg agacggccgc cctccgcgac
     3661 ctcggacgcg tccaggactt cacgaacgcg ctgacctccg agggcttccg cttcgctctc
     3721 gacgatttcg gggtcggctt cagctccttc cgctacctgc gcgacctgcc gatgtcgacc
     3781 ctcaagttcg accagagcta cgtgagggac ctgccgcaca agatggagaa ccgcgtcttc
     3841 gtccgcggca tcgccgagat ctgccggggc ttcggggtga agacggtcgc ggaaggggtc
     3901 gagacgccgg agatcctcga gatccttcgg gagctcggcg tcgaccgcgc gcagggctat
     3961 cacgtcggcc acccgtcgct cgagctcccc ggccgctcga gcgaggcgac ctgaggggcg
     4021 ccttcgcacc gcgggcctag gatggggggc ggaggtccgc gctggcgaac gctcgatccc
     4081 gcccgatcct caccctcctg gaagacgcgc tcgcgtgcca ggcggagcgg ctccgcgggc
     4141 tcgaggagcg cgcccgccga ggaggcgacc cggccctcgt tcacgacgtc cgggtcgcgc
     4201 tgaggcgcct cgagacgacg gcccgcctct ttcgcggctt tccggaagcg aaggacggcg
     4261 acgcgcaccg gatcgcggcg cgggacgttc gccggcgcct ctcgtcgctc cgttccgagg
     4321 aggtcggccg cgcgctcctc gcggagcggg tcgaggacga gtgcctcctt cgggcggtct
     4381 tcccggacga gctccccgcg ctccgcgtcg accccgcgga gatcgcgcgg ctccggaagg
     4441 acgtcgccgg gtggcgacgg cgcctcgccg cggccctcgg agggcccttc gccccgcgcg
     4501 cgcaagccgg cagcgccctc cggcggcgcg ccggccggcg cttctcccgc cgcctcgagc
     4561 gcctggccga gcttctccct cccacccccg ccacgctcca cgccgcgcga atcgcggcca
     4621 agcgggctcg ctacgccctc gagctcctcg acccgcccgc gccggggtcc gcgccgctcc
     4681 tcgatcgcct ccgggtcttc caggacgcgg cgggcgaggc gcacgacctc ctcgaggtcg
     4741 ccgcccgcgc gagggccgcc gcgcgggatt cgtccgatcc gcgtctccct tccctcgccg
     4801 ggtgtctcga agaggaggcg ctcggcgccg ccgtgcgggc ccgacggcga ggggccgccc
     4861 tgcgggagcc gctccggtcc ttccggaagg cgctccggaa gctcgagacg cggtaggctt
     4921 gccgcgaatg tcccgtcttc gagaagccgc ccgcctcgcc gccgccctcg tcccgctcct
     4981 cgccgtcgcc gcaacgccgg cccctccgcc cgagccgccc cggcggatcg tcgtcgtctc
     5041 gttcgacggt gccggcggtc tcgacttcgt gcggcaccgc gaggcgctct cccccgacgg
     5101 cttcctgcgg gcggaacatg agggcgtttc cgcggagagg ctgcagaccg tgacgccgtc
     5161 cctcaccgcg gtcgcgcacg cctcgatctc cacgggcgcg ctcccgcagg cgaccggcat
     5221 cgtctcgaac acctactggc ctgcgggcgg gccgctgaag gcccgcgccg acggcttcga
     5281 ggcggagccg gcggtcgaga cgctctggga ggcgctcgcg cgccagggac accgcgtggc
     5341 cgcgctgtcc tggccgggag tctccggcgc gtcgacgcgc tcgtcgacgc ccgtcggcct
     5401 gcgatgggcg ccgcttcgct cgcgcggatt cgtctggcga gggccggagg aaggagcggt
     5461 ccttcccgac gcggcgatcg ccctccctcc ggaggtccgg agctactccc cgccccggct
     5521 cgtccggatc gagccgcccc gcggacggga cggcgcctcg ccgcgccccc tgctgttcgt
     5581 cgtggtcgac acgcgggacg acggccgccg ggagtacgac ggcctcgccg ccctcgacga
     5641 ggccggcgag ctcgaaggac gcctccgtcc cggcgactgg ctcgcgctct ccgagaagac
     5701 cgacggcgtt gcccggcgcg gccgctggat caagctgctc cgcctcgcgc cggacgcgtc
     5761 ccgcgtcgag ctgtacgtcg gcggaatcgg acagaccgag gcctggcccg acgacttcca
     5821 gaggacgctc gatcggcgct gcgggttctg gccggggccg ccggacgagg ccctcctctc
     5881 ggggcccgag cccgacgtga ggtccttcct cgagatggcc gcacgcttca cggagttctt
     5941 caccgcggcc tacgaggtcg ccgagcggcg cggcgactgg gacgtcctgc tcggctacca
     6001 gccgctcctc gacgaggtcg gccactccct gatgcccccg gacccgggga ccgccggctt
     6061 cgacgcggcc ggcgccgagc gggccgaagc cgcgatgacg gaggcgtggc ggatcgccga
     6121 ccgcgccgcc gcccgctacc tgaggttcct ggagctcggc ggcgacgtcc tcttcctctc
     6181 ggaccacggc cagcgtccgg tccggcgcgt cgtccatccg gccgagctcc tccgccgcca
     6241 gggctggatc acgaccgact ccctcgccgg caggccggtc gtccgccccg actcgcccgc
     6301 ggacgtcgcc gtttccggcg ggaccgccct cgtcgtcctc aaccgcgccg gggagatgcc
     6361 gggaggcgtc ctgtcgccgg acgaaggcgc gggggtcctc gccgacatcg cgagctacct
     6421 gcgcgacctg aaggacgaga cgggggcgcc cctgttcgag accgtcgccc tgcgcggcga
     6481 agcggcgacg ctcgggctcg accatccgaa cgtcggagac ctcctcctcc tggccgggaa
     6541 gggcaccgct cttcggggcg gcttcccgaa gctcggagcc gaagcgccgg tcctcctccc
     6601 ggccgacgtc gcggggcagc acggctacgg acccgacccg gccctcgacg gcatcttctt
     6661 ccacgtcggc cgggggctcg agccggcccg cctgccgctc gtccgcgccg tcgacgtcgc
     6721 cgcgcgcgtc gccgcccggc tcggcctctc ccctccgggg gcgaggtgac gccgcccgtc
     6781 gtctccgtcc cgctcctttc gagctccgcg cggcgaggcg aggcgcagcg gcatctcgag
     6841 cgccacccgc tcggcggcct ccgcggcgag gcgccgggcg aggacgcgac gcgcgtgggc
     6901 ctcctcggcg gcctgcggca cctgcacgtc ttcttcgagg ttcgctccga gccgccgctc
     6961 cgggtccgct gccgccggct gcacgagaac gtctgggagg acgagtgcgt cgagctcttc
     7021 tgggccgacg ccgccgagcc ctcccgctac ctcgagatcg tcgtgaaccc ggagggggtc
     7081 cactacaccg cccgcgtcgc gaacccgcac gcgagccgcg agacctggaa cgtcatccgc
     7141 ggcgtccccg tgccgggcct ccgggttcgc gtcgagggga cgccggcgga cgagccgtcg
     7201 acccgctgga cgagctggcg atgcggcatc tcggtcccct gggccgtgct gacccctccc
     7261 ttcggccgtc cgcggccggg agacgagcgg cgcgggaacc tcacacggat cgcccgcggg
     7321 aagacgcttc gctacgagac gctcgcgccg acgggacggg cgaccccgcc cgatttccac
     7381 gtcccgtcgg ccttcgccac gtttcgattc gtcgaaatcc tgtaacctcg ggcgcgatgg
     7441 agaggccttc cggtccggtc gtggccgtgg cgacgacggc cggcgctccc gcggacctcg
     7501 agctcgtcga gcgaatccgg caaggggacc aggccgctct cgacctcctc tacagacggt
     7561 actccgcgcc ggtctactcg ctggtctgga aggtcctcca gagctccgag gaggccgagg
     7621 acgtcgccct cgacgtcttc tggcaggtct ggcgtcaggc cggacgctac gacccctcgc
     7681 gcggcgcccc gcccgcgtgg atcttcacgc tcgcccgctc ccgcgccatc gaccgtctcc
     7741 gcgcccggaa gcgtcgcgag gaccggaccg tctcgatcga cgaccccgag ctgaacatcg
     7801 acccgctcga cgagaaggcc gccccggacg aggtcgtctc cttccgtcag aaccgcgacg
     7861 ccgttcgggc cgcgatgtcg aagctctccg ccgtccagcg cgaggcggtc gagctcgcgt
     7921 tcctccaggg gatgacccac gtcgagatcg ccgagaagct cgggcagccg ctcgggaccg
     7981 tcaagacccg catccggcag ggcctcatcc gcctccgcaa gcacctcgac tgaggagccg
     8041 atgcgcccca cgcacggcga gcggcccggg ccgcttcccg cggccctccg tccggccctc
     8101 cggcgctggt tcctgaagaa ccgccgtgac ctgccgtttc gccggacgcg cgacccgtgg
     8161 gcgatctggc tttccgagac ggtcctccag cagacgaccg tcgccgcggg ggcaccgcgc
     8221 ttcgagggcc tcctcgcgct cttcccgacc gtcgaggcgc tcgcctccgc tggcgagcgg
     8281 gagctcctcg cggcctggag cggcctcggc tactacgccc gggcgcgcaa cctccaccgc
     8341 gccgcgcgcc tcgtggccga gctcggaggg ggcgtgccgc gcaccgtcga ggagctccgc
     8401 gcgcttcccg gcgtcgggcc gtacacggcc gcggccgtcg cgtcgctcgc cttcggcgcc
     8461 ccggtccccc tcgtcgacgg caacgtctcg cgggtcctct cgcgggtggg agcgattccc
     8521 ggcgacggcc ggacgggcgc ctccggcgcc gccgtcgccc gggcggccga ggccttcctg
     8581 gaccgccggc gccccgcgga gcacaacgag tccctcatgg agctgggcgc cctcgtctgc
     8641 ctcccgcgcg ccccgctctg ccccggttgc ccgctgcggc ccgcctgccg ggcccacgcc
     8701 ctcggccgcc cggaggcgtt ccccgcgccg cgcgcccgca aggccgccgt ccggctccgc
     8761 ctcgccgccg gcgtcgcccg ccgggccggc cgcctcgtcc tcgtcgacga cgcccacctc
     8821 gtgcgcggcc acctcgtcgt cccgctgctc gagcttgccg cgggggccga cgcggccctc
     8881 cgtctccgcg cggcctggcc ctcactcgcc ggccgccgcg cgaagaggct ctctcccctc
     8941 ggaaccgtga gacacgcgat cctcgagcga cgctacgtcg tcgaggtctt cgccgtcgag
     9001 gaggccggac cgacggacga gagagtcccg gtccggcccg tccacccccg cgatctcgag
     9061 tcggagcccc gggggggcct cctgggaaaa gtcctcgcgc tcagcgcttc cgcgggctct
     9121 ccggctccgc cgggacgagc gcgtcccgct cgagctccac ggaaaccgac cggtggatga
     9181 agcggaccga gacgatgaaa cggctcttcc ccttcacctt cgtcacccga cccgtcatcc
     9241 cgaggaacgg gccctccgcg atccggacga ccgagccctt cgtcaggtcc ggccacggga
     9301 cgagcggcac gcccatctcc tggaggcgtc ggacctggcc gaggtcggcc gccagcccct
     9361 cctggtcggg gacggcgagg acctggaggc agaggttcgt cttcaggagc tcgagccgct
     9421 cgtgctcgag gtcccccctg gcgaagagat agccggtgaa gagaggcacg tacgcgaccc
     9481 gcccccggcg gtcgcgcggg ctccgccgct cgcagagcgg caggtagaag gggatccccc
     9541 ggcgccggca ctcgcgggcg aggagcttct cctgcctcga cttcacgtgg acgacccacc
     9601 aggggaagcg ctccgccggc agggagaaga gctcctccgg gaagaggtcc ggctcgggct
     9661 tcaggatcgg cacgacctca ctttaccaat cgcccgcccc ggctatcctc cgcgggatgg
     9721 cgaagaggac gggagacgcg acgacgggag gacgggagct cctggcgccg ctcgggacgc
     9781 tcgtcgcgct ctcgatcccc ttcttcgtcc tgacgctggc gtccgcggag gccacccggg
     9841 acgcgaagct cgccgcccag gcggcgggag gcgtcctcgt cctcctgggg ctggcgtacg
     9901 ggaggccctc cgcagggcgc gagcccgccg gccgaggagc gcgcctcgcg ctcggggcgc
     9961 tcgtcgcgtt cctggcgctc ttcgcggcgt cggcagcagc cggtctcctg cgcggacggg
    10021 acccgctcga cctccttccg ctccttcccg cgctcgccct cttcgcctgg ggagcgaccc
    10081 gacccggaga ggagagcgcc gggcgggcgc tcgcgctcct cgtcggctgt ggcgccctca
    10141 cggggctcct cgccgccctc cagcgcttcg cggggctctt ccggcttccg gtcgaggcgc
    10201 ccgagccgcg cttcttcgcg acggccctga tcgggaaccc cggcgacgtc ggggcctccc
    10261 tcgtcgtccc ggcgctcctc gccgcggcct cgctcgtccg gggcgagcgg cggctcgccg
    10321 ccgctctcgc cctcgccgcg atgctcgccg gcctcgccgc ggctgccacg ctcgcgccgc
    10381 tcgcggcctt cggggcggga gcggcgatcc tcgtcctcgg cgacctgcgg cgccggctcc
    10441 tccccgcgct cggcgccgcc ctcctcctcc tcgggctcct ccaggccggg ggcgtcctgt
    10501 cgcgcgtctc ggagaagctc tcctccggcg cgatcgacga gctgacgacc cagcgcgaca
    10561 tcggcgtcct cgcggcgctc gagacgatcc gcgcccaccc cttcctcggc gccgggcccg
    10621 gcggcttctc cgccgacttc gcgagggcgc ggctcgccgc ggaggagagg ttcggccggc
    10681 gcctcgtcca ccggtccgcc tcggcccact tcgacaacgc ccacagcgac ccgctgaccg
    10741 tcgccgccga aaccgggctc ccggcggccg ccgcgctcgc gctcgcgctc ggggcgctcc
    10801 tcgtgcctct cgcggccgcc gcccgccggg agcggtcccc gggccggccc gaaggcctcc
    10861 ccgcggaggc gctcctcgct tccctcgccg cggtgctcgt cctctcgctc gcgaacttcc
    10921 cgatccagat cgtcccggtg tcgggtccgt tcgcgctcct cgcaggcctc gcgctcgcgc
    10981 gcgtcggagg ctccttcccg ctcccctccg ggcgcgcggg gcgcacggcc ctcgcgctcc
    11041 tcgcgctcct cctcgcctcg ggcgccgcgg tccgcctctc cggcggcctc gcgctcgcgc
    11101 gcgtcgagaa cgctctccgg ctcgctccgg gcacgtccgg cgcgacgcgc ggcgagctcg
    11161 tcgcctcggc gctcgtcgag gcccgccggg cggtcgcgct ccgtccccgg tccgccaccg
    11221 cccacctcgc cctcggttcc gcgcgggccg cggcggccga cctccccggc gccgtcgcct
    11281 cgatggagcg ctctcgcgag ctcgaggagc gcgccgagac gctgctgaac ctcggccgcc
    11341 tcgcgatcgc ccgtggggac gaccgcgcgg cccgcccctt cttcgtccgg ggcgtctggc
    11401 tcttcccgcg cctcgccgca gccatcccgc cggccggcga cccggacggc gtcgtcgccg
    11461 aggtcgcgcg cctcgaggcc gcgctcccgt ccggcggcgc tcctccgccg ccccctccgc
    11521 ctctcagact ccgctgaccc ggaggaccgt cgtcaccgtc cggacgagga cgaagaagtc
    11581 gaggacgggg ttccactgga acaggtagcc gaggtcgtan nnnnnnnnnn nnnnnnnnnn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnng cgaggaccgg tcccgtctcc ctctcggcgt ccgcccgcat
    11761 cgtccggatc ttccagacgt gtagctcccg gcccccgcgc ccgacgcgac gctgccggat
    11821 ccaggccggg cccggcgtct cgaggacgac gacgagcgag acgaccgcga ccgggaccgc
    11881 cgcgagggcg gcgagcggga agcccagcac gacgtcgagg agcctccgga gggcgaacgc
    11941 gggcgccgac gctccccggg cggcgacccg cgtcaggggg aggtcgccga ggcttctcgt
    12001 cgccacgtgc gaggcgacga cgtccgcgag gccgggaagg agccagagct cgaagcctcc
    12061 cgccgcgctc cgctccacga gagcggcgaa cgcctcgcgc tcgcgcgggt tgcccgaggc
    12121 gaggacgacg tggtcggcct ggcgcggcgc gcccggctcc cggctcaccg cgatcgcccc
    12181 gaccgcgtcg ggctcccagc cgacgagcgc gtacccgggc gcctctcccg cttccagggc
    12241 ggcggcggcg cgacgggcgt cctccccgtc gccgaggacg aggacacggc ggacgggcgg
    12301 cggcgccgcg cggtccacga cgctccggaa gagcgcgtgg agagcccacg cggccgggac
    12361 gtagaggagc gcgaccgtcc ggggcagggc gcgggagagg aggaagaacg cgccgaccag
    12421 gaagagcgcc gtcaggaccg ccgcgacgaa gaggccgccc ctctcgcgga gggtctcgag
    12481 cggctcggtc caggtcgcgg cgagcgagtg cgcgaggagc gcggagagcc cgacgacgac
    12541 gagccacggc cacgggtcga gcgggacctt ctccgccggg aggaccccgt ccgtccccgg
    12601 cagcgcgacg acccggcgga gccagacgac cgccgcgaac gccgcgacgg cgccgaggag
    12661 gtccccggcc gcgagcaggg cgcgccgggc cgggctcgcg tgcctcacgc gccctccgcg
    12721 gcgcggctga ggagccgcgc gagccggcgc gcgatctctc cgcgatcggc gtgcgccacg
    12781 gcgaacgcgc gccccgccgc gccgagcgcc tcggcgctct ccgcgtccgc gaggaggtcc
    12841 gacacctcgc gcgcgaaggc ttccggatcg gccgcggtgc ggagcggggc ggcgacgccg
    12901 tcgaggaccg cgcgggcggc ggcggccgga gtcgcgacga ccgcgcagcc ggcctcgagc
    12961 gcctcgtaga ccttgttcgg cgtcccggtc cccatgtcga cggggacggc cgcgacccgc
    13021 gcggagagga ggagcgccct catgtcggag accgggctca cgagccgggc cgcgggcgtg
    13081 ccctcggcga gcctccggag cgaggccggc gcgtcggccc cgccgacgac gagctccgcc
    13141 cccgggacgc gcccacgcac cctcggccag atctcgcgca gcaggagctc ggcggccacg
    13201 gcgttcggac ggtaccccat ccttccggtg aagacgacga cctgcgcgcg ctcccgcgga
    13261 gggggcggtc cgatccgggc gccgatcgcg atcgcctccg tcccctcggg gagcgccgcc
    13321 gcgtcggccg gcgtcgtcgc gaggaggagc cgcgcgcccc gcgcggcctc ccgctcggcg
    13381 cgcatcagac gcgccccttc cgccctccag tagagacgcc gccagagcgc ggggtcccgc
    13441 tccgcgttct gccgcgccgc ttccgagagc gcgtcgacgt agtcgaccac gagcggggcc
    13501 cgcgggagga gcgtccggag gcgcggctga aggcgcgcca ggaggaggac gacgaggtcg
    13561 aaccggccgc accccgcgag cgccctctcc caggccccgt cgaagagcgc ggtctggagc
    13621 ggccagcccc ggacgaaagc gcgggtgaga tggaacggaa gggccgcgac ccggatcggg
    13681 acgccccgca cctcctcggc cccggcgacg gcggcgaccg gacgattcgg cggcacgccg
    13741 ccgacgatcg tcacgcgcgc gccgagctcc gcgagcgcct cgaggtgcag ctcggcccgg
    13801 acccggtctc cggtgaagcc cgttcccggg aggcggggga cgacgaggag ggccctcacc
    13861 gcgtcgcgac cgccccgcgc ggctcggggc ctcggccggc ggcccgcacg cgcctttccg
    13921 acgttccctc ccgcggcccc gtcacacagg ctctcccaag ccgggcgatt ctagagcccg
    13981 cccgcccctc gcgctcggcc ggatgggcgc gacgcgcacg acggcgcccg ccggcgccgg
    14041 cccgcaagta gactccacgc ggaggcgtcg cggggagaat gggctcacag gactcggccg
    14101 cagaggcccg gcgcccgcgc cgagagctca cgctcctgcc gtcgctcggc gccttcctcg
    14161 tctccgccgg gctgttcctc gctcacgcgg cgttcctctt cccgcttcac ctcgacgacc
    14221 cgtacatcac gttccggtac ggccggaacc tcgcccgagg cgacggcctc gtctggaacg
    14281 tcggcgaggc gcccgtcgag ggcttcacgt cgcacgcctg ggtcgtcctg gcggccctcg
    14341 ccgaggcgag cggctctcct ccgctcctcg tcgcgaagct cgcaggcctc gccgccgggc
    14401 tcctcgtcct cgcggtcctc gccttcggga cacgccgcct cctgccgaac gcctccgacc
    14461 gcctcgccgc ggctctcctc ctggccacgt ccacgtcctt cgtgtcgatg agcgcctcgg
    14521 gaatggagaa cgtggcgttc gcggccgccg cgtcgctgct gattcccctg ctggggccct
    14581 gggacgcgcc cgacgccgcg ggccaggtca ccccgcgacg ggccgccctc gccggcctcc
    14641 tgtcggggct cctcgtgacg atccgccccg cggggcacgt cttcgtcgtc gtcctcctcg
    14701 gcgcgctcgc cttcgccgcc cgcgcgactc gcgaacgcag gcctctcgtc gctgccggcg
    14761 tcgcggcagg cgccgtcctc gtgccgttcc acgcctttcg cctcgcgacc ttcggaagcc
    14821 tcgtcccgct cacgtacctg gccaagcaca cgggcgagcc gttcctcgag cgcgtcgcct
    14881 cgggcctgcg ctggctcggc gagcggggtg gcccgctcct gccgatcttc ttcctcgcgc
    14941 tcctgtcggc cctcgtgctc ctccgcggcc gggcggcgcg cgcctccgcg gcgctcctcg
    15001 ccgtcttccc cgcctacgcc gtcgcggtcg gaggcgactc gtcggcgttt cccggagccc
    15061 gcctcctgct taccgtcctg ccgttcgcgg cagcgctcgc cgtcgccgcg gtccgccgcg
    15121 tcacgggcgg cggcctccgc gcggcggcgt tctcggggct cctcctcgcg tccttcacgc
    15181 tcgcccaggt cccggacatg acctcgctcc ggcgcggcct cgccctcgac cgccgttcgg
    15241 gcgcggccgg cgggccggcg cgcgcggcct tcgaccacct cgccgagctc ctcgacccca
    15301 ggcctctccg gctctcctcc tacctggtcg tcgcgacgcc cccgggcgag cggatcgcgg
    15361 tcccctgggc cgggcgtgtc ccctacgaga cgggcctcgc cacggtcgac atgctcggtc
    15421 tgaacgacgc ccacatcgcc cgcctcccga agctcgaccg gggcgtcgac gtgaagtacg
    15481 acccggacta cgtcctgtcg aaacgcccct ggtacgtctg cgagaacttc gtcctcggag
    15541 gagtcccggt cacggccctc gcgcgcctct ccgacgacca gctcgcgcgg ctcggagcct
    15601 tccgggccgg tcagcgggcc cttctccgct cgcccctcct cgcccgcgac tacgacgtcg
    15661 atgccgggat tccgaacagc gccgggggct cgatcacctg cttccgacgg agggagccgg
    15721 cgcgctgagc ggcggctccc cccggggcgg cggcgcggcg cgcccggcgc tcggacctcc
    15781 cgccggcccg agccagatct cgaagacgcc ggccgccgcg atccggcgcg cgccgaccgg
    15841 cccgaggctc gcgagcagcg cccgccccgc ccgcgtcgcc ggcgagaaga cgtagagcga
    15901 cggcccgccg cccgctccgt cgacggcctg gctcgcctcg gccctcagcc gctcctcgga
    15961 gggggcggcg tcggagtacc agccgggatg gagcgccacg tccgacggga agagagtcac
    16021 gcggcccgcg agaccctccc gggcgagacg gtagtcgagc tcgggccccc agagctccgc
    16081 cgcgacgacc ctcgccccgc cgcggacctt cggcccgagc gcggccgcga gctccgaggc
    16141 cggcgtcggc cggacccacg cggggaggga cagggcgagg acgacggccg acgccgcacc
    16201 cgacgcgagc gccacctcgc cccagcgcgc ccgggccagc acgaacgctg ccagcggcag
    16261 gaaggcgagg gccgtccgat cgggcaggag cgccgagccg ctgaaggcca ggagcggaag
    16321 gagaaggccc agcccgatca gcaggggcgc cgccgcgggc agccgcgcgc cccggccgcc
    16381 gacgatcgcg aggccgagga cgacgagggc gagcggcccc gcgtacctcg ccggcccggg
    16441 ctcgaccgga agacccagcc cgaggttcgc ggccgactgg agcgcccgct ccccgagcgg
    16501 ccgagcctcg ctccaggcca tcgaatcgcg cggctgcccc agcgcgacgg ggagccacgc
    16561 cgcggcggtc gcggccgcga ccacacccgc gagggcgacg agccggcgcc gccggcttcg
    16621 cgcccacgcg gagacgagga cgcccgcgac cggcagggcc gccaggtagt gcgtcaggac
    16681 cgcggcgccg ccgaggaggc ccgctgcgag cgccgtccgc ggacgcggag cctcctccag
    16741 ctcgaggatc ctctcgaacg tcgcgagcgc gaggagcgac gcgagcgcgt agccgcgccc
    16801 ctcggagccc gatgcgacgg cgaggggaaa gagaaggaag agcgccgcgg cgacgagccc
    16861 cgtgcgagcg gcgccgcagc gccggccgat ccgcagcatc aggggcacgt gcaggaggga
    16921 cgcggcgacg gagaggaggc gaatgacgac gtcggccgcc cccggcgccg ggaacggcag
    16981 gaggagcagc ctcgccgcga ggtaatggaa cggcgggccc gagtcgagac ggagggccgc
    17041 caggagatcg gcgagaggcc gtcgcgccag cgtcagcgtg aagacctcgt cggcccacag
    17101 agggcgggcg aacgccagga agaggcgcgc ccccgcgccg acgaggatcg cgaagacgaa
    17161 cgctcgccga agagtccgct cttccgcgtc ctcgctcgtc gtccgcgcgc cctcgtctcc
    17221 ctccactccg ccgactttcg ggccgcgctc agtcgaacgc aagggcggtc gcgcattcgg
    17281 acccgatcgg tccggatcaa atacaaccgg accgatcctg ccctgtttga aaacaccatt
    17341 tcctcgacat gaggtgcccg cgggactacg atttcggttc ggggcagacc ctccttgatt
    17401 tcgaaacgcc gcgctcagca cgactccgtc cgcctggcta cggccgggct ttttcgtggc
    17461 ccgacatccc aacggcgctc cgccgcccgc gaggtccgcg agcggccggc gcgccccgcc
    17521 tgaggaggct ccatgcaggt ccaggtctcg tccccccctc cgcccccggc caccgcctgc
    17581 gcgtgcggcg aagcgaccga ggcggagaag ctcgcgcgca tcgcggaggt cgtcgaggag
    17641 ttccgcgaca agcccgggag cctcatccag atcctccacc tcgcgcaggg gatctacggc
    17701 tacctcccga tccacgtgca gcggctcgtg gcggagaagc tcgacatccc cgtctccgag
    17761 gtctggggcg tcacgacgtt ctactcgttc ttctcgacga agccccgggg cgagtactcg
    17821 atccgcgtct gcctcggcac ggcctgctac gtgaggggcg gcgtcgagat cgtgaagcgc
    17881 ctctcgcaga cgctcggcgt cggcgtcggc gagacgaccc ccgacggcaa gttcacgctc
    17941 gaggtgatgc gctgcatcgg ggcctgcggc ctcgcccccg cgctcctcgt gaacgacaag
    18001 gtcgtgaggc aggtcaaccc ggacaagctg aaccgggtcc tcgccctctg cgagtgatga
    18061 ggaagcggaa gatgaccccc gagccgaacc tcacccgccc cgcgagcaag gccgacctcg
    18121 acgcgatcgc cgcccgctgg cacgccgagc agcaggggaa gaagcaccag ctcctcgtct
    18181 gcgccggcgc cggctgcgtc tcctccggct gccacgccgt caagcacgcc ctcctcgccg
    18241 ccctgaagga cgccggcctc gagaaggaga cggccgtcca cgagacgggc tgcctcggct
    18301 cctgccacct cgggccgacg ctcgtcgtcc agcccgacgg cgtcctctac accgagctca
    18361 agcccgagaa catgaagggc atcgtgaggc agcacctcgt ccaccgcgag gtcgtcgagc
    18421 agcactgctg gaccgatccc gagacgaagg agcccgtccc gcacctctcc gacatcgagt
    18481 tcttcaagcg ccaggtgaag atcgcgaccg cccgctgcgg ctcgatgccg tacgcctcga
    18541 tcgaggccta catcgcctcc gacggctacc agggcctctg gaaggcgctc tcgacgatga
    18601 ccggcgagca ggtcatccag gagatgcgcg cctcgggcct ccggggccgc ggcggcggcg
    18661 gcttcccggt cggcgccaag tggcaggccg gcttcaaggc gaagggagcc gagaaggtcg
    18721 tcgtctgcaa cgcggacgag ggcgaccccg gcgccttcat ggaccgctcc ctcctcgagg
    18781 gggacccgca ctccgtcctc gaggggatgc tcctcgccgg ctacgcgatc ggcgccagcc
    18841 agggctacgt ctacgtgcgc gccgagtacc cgatcgccgt cgagcgcctc gaggccgcga
    18901 tccggcaggc gcgcgcgtgg ggcttcctcg gcccgaacgt catggggacg aagttctcct
    18961 tcgacgtcga gatccggatc ggcgccggcg cgttcgtctg cggcgaggag accgcgctca
    19021 tgcactccgt cgagggcaag cgcggcgagc cgcggcagaa gccgccgttc ccgttcgagc
    19081 gcggcatctt cggcaagccg acgatcatca acaacgtcga gacgctcgcg aacgtcgcgg
    19141 cgatcgtgaa gaacggcggg gcctggtacg cctcgctcgg gacgtcgaag agcaccggga
    19201 cgaaggtctt cgccctcgcc ggcaaggtcg agaacaccgg catcgtcgag gtcccgatgg
    19261 ggatcaccct cggcgaggtc gtccacgaca tcgccggcgg catcccgaag gggaagcgct
    19321 tcaaggcggc ccagacgggc ggcccgtcgg gcgggtgcct gacgcgcgag cacctcaaca
    19381 ccccgatcga ctacgactcc ctcgtccacc tcgggacgat catggggtcg ggcgggctca
    19441 tcgtcatgga cgaggactcg tgcatggtcg acgtcgcccg cttcttcatg gacttcgtcc
    19501 aggacgagag ctgcggcaag tgcgtcccgt gccggatcgg gacgaagcgg atgctcgaga
    19561 tcctgactcg gatcacgaag gggcagggaa aggacggaga catcgagctc ctcgaggagc
    19621 tcgccgcgaa ctgcaaggag acctccacct gcggcctcgg ccagacggcc ggcaaccccg
    19681 tcctctccac gctccgcctc ttccgcgagg agtacgagga gcacatccgc gacaggcact
    19741 gccgcgcggg cgtgtgcgcc gacctcttca agtcgccctg ccagaacgcc tgcccggcgg
    19801 gcgtcgacgt cccgggctac gtcgccctca tcgcggcggg gcgcccgcgc gacgcctacc
    19861 acctcgtccg ccgcgagaac cccttcccgg cgatctgcgg ccgcgtctgc acccacccgt
    19921 gcgaggacaa gtgccgccgc gcgcagctcg acgagccggt cgcgatctgc gacctgaagc
    19981 gctacgccgc cgactacgtc ttcgcgaacg acgagccgca ccgcgacctc gtcttcccga
    20041 agaaggccga gtccgtcggc gtcgtcggcg cgggcccctc gggcctgacc tgcgcctggt
    20101 acctcgcgcg cctcggctac ggcgtcaccg tctacgagtc gctcccggtc gccggcggca
    20161 tgctcgcctc cggcatcccc gagtaccgcc tcccgaagga ggtcctcgcg aaggagatcc
    20221 ggctcgtcga gcaggtcggc gtgaggatcc tcaccggcgt cgaggtcggc aaggacgtga
    20281 cgttcgagga gctccagaag cagcaccagg cgatctacgt ggcgaccggc acccacttcc
    20341 cgaacaaggc cgagatcccg ggcgaggaga aggccggcat cgcgcacggc gtgtcgttcc
    20401 tgcgcgaggc gaacctcggg aggaaggtcg aggtcgggaa gaacgtcgtc gtcatcggcg
    20461 gcggctccac cgccttcgac gcggcccgga ccgtcctccg cctcggcgcg gagaaggtga
    20521 ccgtcctcta ccgccgacag gtcgaggaca tgcccgccga cccgcgcgag atccgggagg
    20581 cgcgcgagga ggggatcgag atcctcccgc tcgtcgcgcc cgtcgagttc ctcgggaacg
    20641 ggcgggtcga gggcgtcaag tgcgtgaaga tggagctgaa gggcttcgac gacgccggcc
    20701 ggcggaagcc gaagaccgtc gccggctccg agttcgtcgt cccggccgac atggtcatcc
    20761 ccgccatcag ccagcactcc gacctcccgt tcatcgaccg ggacgaggtc cagctgacga
    20821 agtgggggac gctcgtcgtc gacaaggaga cgatgatgac ccgcaagaag ggcgtcttct
    20881 ccggcggcga cgtcgtccgc gggcccgacg tcgcgatcca ggcgatcgcc gacggcaagc
    20941 gcgccgctcg cgccatcgac cgctatctcg gcgggaccgg cgtcctgaac aagggggagg
    21001 agatcccgat cccgcgcccc accgacgaga ccgacgtcgc cgagcacgag cggttcccga
    21061 tgcgcttcct cgagcccgac tcccgcagga aggggttcca ggaggtcgcc gcgggcttcc
    21121 acaagctgaa cgccatcgcc gaggccatgc gctgcctccg ctgcgaccgg cggtaggaag
    21181 agagccatga cgaaccccgt tcacctcgtc atcaacggcc gcgacgtgag ggccgagagc
    21241 gactggtccg tcctcgaggc cgccaagcac aacggcgtca agatccccca cttctgctac
    21301 ctggagggcg tccaccagat cggctcctgc cgcatctgcg tcgtcgaggt cgaggggatg
    21361 cggagcctcc aggcctcctg caccgtcccc gtccgcgagg gcatggtcgt gaagacgaac
    21421 accgcgcgcg tgaagaaggc gcgcgaggtc ctctacgagc tgatgctctc ggaccacccg
    21481 cgcaactgcc tctcctgcta ccggaaccag aactgcgagt tccagaagct gggcgaggag
    21541 atcaagatcc ccgactgccg cttcgagggg gagcggtcga agaccttcgt cgacgactcc
    21601 agcccctccg tctaccgcga cacgagcaag tgcatcctct gccgccgctg cgtcaccgtc
    21661 tgcagcgaga tccagggggt cggcgtcctc ggcgtcaaca accgcggctt cgggacgacg
    21721 atcggccccg ccgccgacct cccgctcggc acggcgaact gcgcgaactg cggccagtgc
    21781 gtcatggtct gccccgtcgg ggcgctcctg acgcgcgccg acatgacccc cgtctggaac
    21841 gccctcttcg acccgaaggt ccgcacggtc gtccagacgg cccccgccgt ccgcgtcgcc
    21901 ctcggcgaga tgttcgggat ggagcccggg acgatcgtca ccgggaagat ggcccacgcc
    21961 ctccggctcc tcggcttcga cgacgtcttc gacacgaact tcgccgccga cctgacgatc
    22021 ctcgaggagg ggacggagtt cctccaccgg gcgctcgccg cggtgcgcgg ccaggacgtc
    22081 gtcctcccga tgatcacctc ctgctcgccg ggctggatca agtacgtcga gcacgagttc
    22141 cccg
//
LOCUS       DAOTEZ010000012        21950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_1569_length_21950_cov_6.793423, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000012 DAOTEZ010000000
VERSION     DAOTEZ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 21950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21950
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1569_length_21950_cov_6.793423"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4788)
                     /locus_tag="P5164_01240"
     CDS             complement(<1..4788)
                     /locus_tag="P5164_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRY42543.1"
                     /translation="MPFAPIAIVGRSCVLPGALDPSALWELVAAGRDVVGEAPEERWG
                     LEKSEVLRDPRGPAPDGAWSDRGGYVSGFEAVWDPAGFGVGERELEGLDPLAHWVLHT
                     AREALRDAGDGRSGTVARPRTGAVFGNLGFPSEGMAAFARSVWTENGRAGEGAGVDPR
                     NRFMSGGTATLLSRALGLGSGAFCLDAACASSLYAIKLACDRLHDGEADVMLAGAVQR
                     ADDLFLHVGFTALGALSRSGRSRPFHREADGLVPAEGASFVVLKRLEDARRDGNTIHG
                     VIRGVGLSNDGRGKGLLAPSEEGQERAIRAAYEEAGLGPEQVSLLEAHATGTPVGDAA
                     EIRSTGAVFAGLGGVALGSLKSNLGHLITVAGVAGLLKVLGAMKAGQRPPTLHAEEPT
                     RALDGSPFRLVEKLEPWPSDGPRVAAVSAFGFGGNNAHLVVSEDSAELDERVGMREAV
                     PAPPPALAVVGLGCAVASAPDRRRFAEALFSNESLLDASGEGRLGPVELDLEGLRFPP
                     KDLAQTLPQQLATLRAATEALAGSGPLPRERTGVFVGMEPDPEVARWGTRWRLARTAR
                     ETGASAEWLRSARDAVVPVLEAAAVVGTMPNIPANRLSSQLDLGGRAFSVQAGEASGT
                     TALSLAMRALRSRELDAALVGAADLSCEKVHSSAVGALADGAPNPPGDAAVVLVVKRL
                     EDAERDGDRVWALIEEQGGADGEGREGGGAPLLLSGEGSTSLVPRFGHAHAASDLVHV
                     AAAALSLHHRRLPGGRPWLPSGAFARAACVAVPNGPSIRLTEAEAHPRRGEDEAPRLR
                     CFAGPDRAAVIDALRSGREGLGAEAAARLVVVARDGNFEDVRARALAHLEKGTPAGPG
                     IHFRERPVGGDLAFVFAGAGAAYRGMGRDLLERLPGLADSLARRSRRLPRALAWSFED
                     DSREPTVLEQLWGASALCQLHAELTRGFLGIVPHAWLGYSSGETNALLASATWTDADA
                     LIEESESSGLFTRDLGGSFAAIERAWGTPVRWASFTVLAPVADVTAAVAREEKVHLAI
                     VHGDEECVIAGDEDGCTRVVAALGEGRCLRLGYPLAVHVPELDTVRDAWLSLHRRSTT
                     PPLPAASRSAARFYTNATGGSYAPTSESCAQAILGQANRTLDLRPTVLQAFEDGVRVF
                     VEHGPQAACGRWIRAILGEREALVVSLDRKGQGLEATLDALAALVAAGVPLRAGALDE
                     LDTLLRGERPSPGSGRSLSFPAHPPAITIPPVPAVGARIGAADAREMTPAPALPPVID
                     EEWGVRPAEPAGLAAPRERSVTPVTPVTLVAPVAPVVPASPASSAPPALVPSAVLDGF
                     RAHVAQLSRIQQDHVLVQQALHERFLSLRGSAMETFLHAASRAGTAQATPAPEAPTVP
                     ATPSVAPAPPVPPVAAVPSPVALVPVPAPTPRRDGELVGPRFDRRQLEVHAGGRISEI
                     FGPAFAAQDAYERQVRMPLPPLLLADRVVGLSAEPASMGKGTIWTETDVTWDSWYLHQ
                     GYMPPGLMIEAGQADLMLISYLGVDLLNRGERVYRLLGCELTWHGDLPRAGDTLRFGI
                     HLDGHAAQGPVRLMFFHYDCTNGDRLQLTVRNGQAGFFTDAELA"
     gene            complement(4793..11119)
                     /locus_tag="P5164_01245"
     CDS             complement(4793..11119)
                     /locus_tag="P5164_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY42544.1"
                     /translation="MKHDPSPSRQPVAVVGVSALFPGSSDATGFWKDILAGTDLIKDV
                     PPGHWLVEDYYDPDPAAPDKTYAKRGAFLDKVDFDALGWGVPPSTIPATDTSQLLALV
                     VAQKVLQDALREQFETADRSRVSVILGVTSAQELLSSMVSRLQRPVWVKSLRESGLPE
                     DEVTAICDRIASHYVPWQEATFPGLLGNVVAGRIANRLDLGGTNCVCDAACASSLSAL
                     SMAVAELQLGHSDLVISGGVDTLNDIFMYLCFSKTPALSPSGDCRPFSDQADGTMLGE
                     GLGMVALKRLEDAERDGDRIYAVIRGVGSSSDGRSKSVYAPVPSGQALALRRAYELAG
                     YGPETVELLEAHGTGTKAGDVAEFEGLRLVFDAAGREDRQWCALGSVKSQIGHTKAAA
                     GAAGLFKAVLALHHGVLPPTIKVDAPNPKLGLPESPFYLSTKARPWVRDARHPRRASV
                     SAFGFGGSNFHVALEEYRGPAPRAARFGTLAKELVVVCGDTPAQVASKARELAKERAG
                     VAGASGLAALARESQEGYRPGASARLAVLAADGDDLARKLLEAAKRIEAAPKEAFSLP
                     DGTAYGAGPCEGKVAFLFPGQGSQYVGMGADLAMGFRTALDAWDRAADLDLEPGTPLH
                     EVVFPRPGFGEEREKSDLARLTSTQWAQPAIACVSLSQLAILEEAGIRPDLTGGHSLG
                     EVVALRAAGVLSSDADLLRVARRRGELMAEAAAIPGAMTALTLPVADVRALLARLAPR
                     VVVANHNGPTQVVVSGSTADVADLETKLKSEGITFQRLPVATAFHSSLVAGACEPFAR
                     FLANVPFASPALPVYSNALAAPHEDAPEALRAALAGQVASEVRFVEMVEAMYAAGART
                     FLEVGPGAVLTGLLGRILDGRPHLAVNLDRKGRNGVETLQLALARLVAAGVPMSPAKP
                     FADVRVDRTEGTAAASKLAVAIDGSNHGKLYPPPGGAASLPAPNPPRPAPLPQQGASA
                     PANPPAAAPQESPMRIETAVPVSSAPSPSHEAPARALAGNPSDWVLAFQETQRQTAEA
                     HAVYQRAMAESHASFLRVAESGMLGLTAMVTGQPLAMPAPMPAKEPASRFVPEQPATV
                     RAAAPAEVHGPAHVPVAAVVETPAPPTAALAAQPAPPVVVPLASAASPTAATSDVDLV
                     GLMLAVVAEKTGYPADMLNLGMDLEGDLGIDSIKRVEILSAVADQVPELRSVDMGHMG
                     SLRTLGAIVEYMKELAPQEPAAPPAKSAATSDVDLVALMLAVVAEKTGYPADMLNLGM
                     DLEGDLGIDSIKRVEILSAVADQVPELRSVDMGHMGSLRTLGAIVEYMKGVGQPGAAS
                     VAAPTTAPATAPVAVPAAPAAVHAAKAAPALGRFALERIAAPATGLAQPGLLSGGEVW
                     VIGSPELNEPLAAELRARGVNARTTSTLPEGATACVYLGGLRDVADVDEAIAVNREAF
                     ALARTLAVRLEKERGLFVTVQDTGAAFGTAPMDPRRAWLAGLPALVKTAGQEWPQASL
                     KAVDLERGGRGADEIARALASELLEGGGEAEVALPAAGGRFTLRSVAKPVTAGAPVIV
                     PGDVVVISGGARGVTAACVEEWARESRARFVLLGRTPLAPEPACCAGIAEEAGLKKAL
                     LAEARGRGESPVPAELGARVHEVLANREIRRTLDAIAAAGGEARYEAAGVEDAQALEA
                     VLARVRSEWGPVAGVVHAAGVLADRNISQKTDAQFDRVFDTKVQGLRALLAATASDPL
                     KLLCVFSSVSARCGNNGQADYAMANEVLAKVAWAESRRRPELLVKSLGWGPWEGGMVT
                     PQLKERFAALGVPMIPLVAGARMFVDELRGAERAEVELVLGGEPRAEALLFAGADARV
                     QELEVKVQRGSHGWLEGHSIKGTPVVPVVLAAEWLSRAARSFRPGLALAALHDIKVLK
                     GIRLGAFDNGGDRFVIQARQTSGGPGTWLQLDVRGSAGTHHYSARAELRPELRAPESE
                     LPELPLGEWSGEPIYRDLLFHRGAFELIEEVEGISDHGAGGRVRGVERAGWPNGTANP
                     WQLDVAALDGGLQVAVLFAQRMLGKENLPTTIGELRSYRSAPSPGVVSVAAYRRKVGS
                     LGVTTDIHFTDDEGRRLASLLGVETHAYPAGSQA"
     gene            complement(11163..12782)
                     /locus_tag="P5164_01250"
     CDS             complement(11163..12782)
                     /locus_tag="P5164_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020509716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PfaD family polyunsaturated fatty
                     acid/polyketide biosynthesis protein"
                     /protein_id="HRY42545.1"
                     /translation="MSSPASSLRWRPNGRPAAFTPAEIGSALEEVRSASHVVRDDGGR
                     VGLGLGGELVPSAANGGAGTFAHVASLPPLYPEWLGDRTFCEAHGTRFPYVTGSMANG
                     IASVRLVSEMARAGCLGFFGSAGLSPARVEAALDELENELGEKGLPWGANLIHSPNEP
                     ELEESVADLYLRRGVTRVEASAYMALTPAVVRYAATGLTTDPAGKARRKNHLFAKISR
                     EEVAIRFLEPPPAEILRRLVERGQLTAEEARLAATLPLAEDLTAEADSGGHTDNRPLT
                     ALLPAILGLRDRVVAGRGYRRAVRVGAGGGLGTPSSVAAAFALGASYVVTGSVNQACV
                     ESGLHESGRALLAEAGIADVIMAPAADMFELGVTVQVLRRGTMFAVRAKKLYELYRSY
                     PSLESIPAAEREKVETQLLRATFEEAWASTRAFWAARDPREVARAESDPRHLMALVFR
                     SYLGLSSRWAIAGEPDRSADFQIWCGPAMGAFNSWVRGSFLEKPADRTAVQVARNLLE
                     GAAAVTRAQQLRASGLAVPPSAFDFRPRPLS"
     gene            12853..13452
                     /locus_tag="P5164_01255"
     CDS             12853..13452
                     /locus_tag="P5164_01255"
                     /inference="COORDINATES: protein motif:HMM:NF037625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRY42546.1"
                     /translation="MKAPRPLRERKKDATRQALLEIGNRLFHRKGFEATTIDEICEAA
                     GISRRTFFRYFPSKEALVFPHRAERLERFVAFLVSAPPDERPIATLRRTAAIFAREYS
                     EHRAQLVAQQKLIHTSPALLAREREIDRDWEMAMSRAFRERAGPGHASELRARVLAGA
                     AIGVIRATMRHWFATDGKEDLARLGEEALACLDRGFSLE"
     gene            complement(13473..14855)
                     /locus_tag="P5164_01260"
     CDS             complement(13473..14855)
                     /locus_tag="P5164_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HRY42547.1"
                     /translation="MPHEKSDLYARRRERFFDRVKEGVALLFATPEQHLSWDGTYRYR
                     PDPDFFYLTGFGEPGSVAVLDAGKRVYRLFVRPKDRERETWEGRRAGVKGAVKAHGAD
                     EAFEVGELEKRLPDLIRGAGTLHYALGLDERKDLLVTSILNRFRREPRVPLRGPVAVC
                     DVTDVIHEMRLRKEPEEIALMEKAAAITVAAHEEARGLARPGRWEYEVEAAINGRFRR
                     LGASGPSYETIVASGANATTLHYVENDRRIEEGDLVLIDAGCEYEGYAADITRTFPAS
                     GRFTPEQEAVYRVVLSALHAATSKVRPGGRFQDVHDAAVDVLVDGLLELGLLEGKRKK
                     VLEKGTYTRFYMHRTSHWLGMDVHDRGRYHDAAGEWRLFEPGMVLTVEPGLYIRPDEK
                     KVPPAFLGIGVRIEDDVLVTEDGHRVLTAAAPKSVEAMCERGERRSARSAGAVTSKAG
                     RVPKVSKPGA"
     gene            complement(15373..16242)
                     /locus_tag="P5164_01265"
     CDS             complement(15373..16242)
                     /locus_tag="P5164_01265"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRY42548.1"
                     /translation="MRLELRLKKAAAENGVPYETVLKDYSIGHVLSAIAAEESLAGTL
                     VMKGGTALRKLHFDDYRFSEDLDFTGRGAPRGDDLEAGLRSAAGRARERMEANGPFTA
                     TVDRVVLREEHPGGQEAFVVRIQFPWQREPLCALKVEVTADEPILLPTESRPILQAYG
                     EELPGHVVCYSLEEIVSEKLRALLQNEERRKKRAWVRPRCRDFYDLWTLLGKPQPGFD
                     ASAVRRQLHAKCEVRGVGFRTWEDFFPEPLVALVGDAWQGDLGGLVPEMPEPGTVVAE
                     LKWLVAKLLEAEG"
     gene            complement(16239..17111)
                     /locus_tag="P5164_01270"
     CDS             complement(16239..17111)
                     /locus_tag="P5164_01270"
                     /inference="COORDINATES: protein motif:HMM:NF020962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HRY42549.1"
                     /translation="MDTRRIRSAKPDRAPYGETHGLAFLGEIEDEGKLVFSSEDARRA
                     GEKLGLGPARVNGLVSRLALAGWLRRIRRGLYATTGRLPGSTPPHDFAIAVALATPSA
                     LSHRTALHFHGFSDQVPRTITCTTTAKVVTPSMRSGTPTAGRSSVWAVDGLRVELVTV
                     TPSRFFGVEEVWIDQRTRVPMTDRERTILDAFSSPARIGGLGEALAILEEHAASLDLP
                     KLAGYAVRFGVAATVKRLGWALESFGAKEDVLAPLLAYPVKMTQPLDPRRARKGPVIP
                     RWKLQDNLKEPARR"
     gene            complement(17181..17411)
                     /locus_tag="P5164_01275"
     CDS             complement(17181..17411)
                     /locus_tag="P5164_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42550.1"
                     /translation="MPRRIRDHARARTGRRTVPLLKNVGETAKECCARLGVRVPKVSD
                     VADIATRAPQGESCRSRFGKWFRISDLTPAAC"
     gene            17778..18710
                     /locus_tag="P5164_01280"
     CDS             17778..18710
                     /locus_tag="P5164_01280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRY42551.1"
                     /translation="MDPIRIVRRLAALYPAETASRLKRAVIEGQVTVNGVVVGDPGAI
                     VTDDDAVVFDTNRKVQRKVDTSLTVLYEDDDAVAVVKPAGLLTHPTEAKEKDSLLSRV
                     STWAAKRGKGRPYISVVHRLDKETSGVLVFARSRAGLVSLQGQLRAHSVDRRYLAVVE
                     GDLEGEKGTFEWPILEEGGRRKRVAREGEKGIRAVTDWKVVERFGIATLVEARLRTGR
                     THQIRIHFSHAGHPLVGDQVYRDPKRPPFPVAFRRQALHAGHLGWEKLDGTKASVSTP
                     APKDFEDLLAALRTRKGKAGRPVKPGAPGPSKKR"
     gene            complement(18715..19356)
                     /locus_tag="P5164_01285"
     CDS             complement(18715..19356)
                     /locus_tag="P5164_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRY42552.1"
                     /translation="MTKLNKAALVGVAVLVTGAFGCATTKQVDKKIAEATAKTDQKIE
                     TVETQVEELQTKQKETETKVDAQGQEIVKVSQAAQEALKRAQEAGILAKGQIVFEQTF
                     TEDRVKFRTGSAELSADAKVALDEFAAKVKELKRPIWMEIQGHTDSTGTTEINDALGA
                     KRAEAVRLYLARKHQLPIQRMTTISYGDTLPADTGKGSKARANNRRVVLVVLE"
     gene            complement(19399..20676)
                     /locus_tag="P5164_01290"
     CDS             complement(19399..20676)
                     /locus_tag="P5164_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRY42553.1"
                     /translation="MKGPLALVVAILAGALSAPAEEPLRLRGGFGRQAPTPMPSPRPT
                     PWPTPTPWAGTFQPVRTAAPTPTAVAPHAASPVPAQPPLATASPAHAPAVRTPAPTAG
                     AIKASPTRPAAAPSAPRPAVPRARPKPARAAADVSVGYVLVPFVVTDRKGRPVAGLKE
                     KDVTLLADGVPVAYDLFQGSSDAPVSYAILLDGSGSMGLAGKMEGARAALRALAAARV
                     PGDDFALYVFAEGEVKEVVPFTEDARAILAAADEVKPWGKTAFRDALALMPEKSLQGK
                     NGSRAIVLLSDGIDNDSELSDEELGSLMEGIEVPVFPLGIRSPGGIAEPPPGQTLEWS
                     LNVAVLSRIARITGGRMAIVDDPALLPEKVLDIQKDLRSQYLVGFSPTGTGPVRYRRL
                     TLRVAGPARPVRVRAGYRGSDPPAFGPHRPGAK"
     gene            20775..21614
                     /locus_tag="P5164_01295"
     CDS             20775..21614
                     /locus_tag="P5164_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42554.1"
                     /translation="MGRRHLATVSSRVEEADAAWSDRAKGSSGGRARVGGIRNVVALL
                     RDELAEEPLSVAARWRLMRALYFEGEYAAAGEKEAKRLFVEGTRVADECLALLRDRAS
                     EGSSRDLSRATPVELVPRLAGDPDAASAFEWGGVVWGMYALAEGMLAAAREGAAGRIR
                     DSAEAVLRLDPGLDDAAGDRTLGRLHHKTPAIPFLTGWASRTEAIRHLRRAASAAPTS
                     LVNRLFLAEALHDLDSSRRDEAIAIAEAVVRDAPDPAWLVEHERARADAKALLKSWRA
                     AGR"
     gene            complement(21646..>21950)
                     /locus_tag="P5164_01300"
     CDS             complement(21646..>21950)
                     /locus_tag="P5164_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRY42555.1"
                     /translation="HRITKAEAEGFYAEHAGKGFFGSLVAYMTSGPVFVAVLERENAV
                     AHWRAVMGATNPANAEEGTIRKEFGQSIERNASHGSANLADAAREVTFFFSQAELI"
BASE COUNT         2988 a         7964 c         8048 g         2950 t
ORIGIN      
        1 cgccagctcc gcgtcggtga agaagcccgc ctggccgttc cgcaccgtca gctggaggcg
       61 gtcgccgttc gtgcagtcgt agtggaagaa catgagccgc acggggccct gcgccgcgtg
      121 gccgtcgagg tggatcccga agcggagcgt gtcgccggcc cgcggcaggt cgccgtgcca
      181 cgtcagctcg cagccgagga ggcggtagac ccgctctccc cggttcagga ggtcgacccc
      241 gaggtacgag atcagcatca ggtcggcctg gcccgcctcg atcatcaggc ccggcggcat
      301 gtacccctgg tggaggtacc aggagtccca cgtcacgtcg gtctcggtcc agatcgtccc
      361 tttccccata ctcgcgggct cggctgagag gccgacgacg cggtcggcga ggaggagcgg
      421 cgggagcggc atcctcacct gcctctcgta cgcgtcctgc gcggcgaacg cggggccgaa
      481 gatctcggag atccggccgc cggcgtggac ctcgagctgg cggcggtcga agcggggtcc
      541 gacgagctcg ccgtcgcggc ggggcgtcgg ggccggaacg gggaccagcg cgaccggcga
      601 cggaacggcc gcgacgggcg gcacaggcgg agccggcgcg acggagggag tcgccggaac
      661 cgtcggggcc tccggggcgg gtgtggcctg cgccgttccc gcgcggctcg ccgcgtggag
      721 gaaggtctcc atcgcggacc cgcgcaggga gaggaaccgc tcgtgcagcg cctgctggac
      781 gaggacgtgg tcctgctgga ttcgcgagag ctgcgccacg tgggcgcgga agccgtcaag
      841 gacggcggaa ggaacgaggg cgggaggtgc ggaggaggcg ggcgacgcag gcacgacagg
      901 cgccacgggc gcgaccagcg tgaccggcgt gaccggcgtg acgctccgtt cacgcggggc
      961 cgcgagtcca gcaggctcgg cgggacggac gccccactcc tcgtcgatca ccggcggaag
     1021 ggccggggcc ggggtcatct ctcgcgcgtc cgccgctccg atcctggcgc cgaccgcggg
     1081 gacgggcgga atcgttatcg cgggagggtg cgcggggaag gagaggctcc ggccgctgcc
     1141 gggcgaggga cgctctcctc gcagcagcgt atcgagctcg tcgagggccc cggcgcgcag
     1201 cggcacgccc gcggcgacga gcgccgcaag cgcgtcgagc gtcgcctcga gcccctgccc
     1261 cttccggtcg aggctgacga cgagcgcctc gcgctcgccg agaatcgcgc ggatccagcg
     1321 gccgcacgcg gcctgcggcc cgtgctcgac gaagacgcgg acgccgtcct cgaaggcctg
     1381 gaggacggtc gggcgcaggt cgagcgtccg gttcgcctgg ccgaggatcg cctgcgcgca
     1441 gctctcggac gtcggcgcgt aggagccgcc cgtggcgttc gtgtagaagc gcgcggcgga
     1501 ccgcgacgcg gccgggagcg gcggggtcgt cgatcggcgg tggagcgaga gccaggcatc
     1561 gcgcaccgtg tcgagctcgg ggacgtggac cgcgagcggg tacccgagac gcaggcagcg
     1621 gccctctccc agcgccgcga cgacgcgggt acagccgtcc tcgtcgcctg cgatgacgca
     1681 ctcctcgtcg ccgtgaacga tcgcgaggtg caccttctcc tcgcgggcca ccgccgccgt
     1741 cacgtcggcc acgggagcga ggacggtgaa ggacgcccag cggaccgggg tgccccaggc
     1801 cctctcgatc gcggcgaagc tcccgccgag gtcgcgcgtg aagagccccg agctctcgct
     1861 ctcctcgatc agcgcgtcgg cgtcggtcca cgtcgcggag gcgaggaggg cgttcgtctc
     1921 gcccgaggaa tacccgagcc acgcgtgggg cacgatcccg aggaagccgc gggtcaactc
     1981 ggcgtggagc tggcagagcg cggaggctcc ccagagctgc tccaggaccg tgggctcgcg
     2041 ggagtcgtcc tcgaaggacc acgcgagcgc gcgggggagc ctccggctgc ggcgcgcgag
     2101 cgagtcggcg aggcccggca gccgctcgag gaggtcgcgg cccatgccgc ggtaggccgc
     2161 cccggctccg gcgaagacga aggcgaggtc gccgcccacg gggcgctcgc ggaagtggat
     2221 tccagggcct gcgggcgttc ccttctcgag atgcgccagg gcccgcgctc gcacgtcctc
     2281 gaagttcccg tctcgagcca cgacgacgag gcgggcggcc gcctccgcgc cgagtccttc
     2341 gcgaccggag cgcagcgcgt cgatcaccgc cgcgcggtcg ggtcccgcga aacagcgcag
     2401 gcgcggggct tcgtcctcgc cgcggcgcgg gtgcgcctcg gcctccgtga gacggatcga
     2461 cgggccgttc ggaaccgcga cgcacgctgc gcgggcgaat gcgccagacg ggagccacgg
     2521 cctcccgccc gggagcctgc ggtgatggag gctcaacgcc gcggcggcga cgtggacgag
     2581 gtccgacgcg gcgtgggcgt gcccgaaccg cggcacgagg ctcgtcgagc cttcgccgga
     2641 gaggaggagc ggcgctccgc ctccctcgcg tccttccccg tccgctcctc cctgttcctc
     2701 gatgagcgcc cagaccctgt cgccgtcccg ctcggcgtcc tcgagccgct tcacgacgag
     2761 gacgaccgcg gcgtccccgg gcgggttcgg cgctccgtcg gcgagcgccc cgaccgcgct
     2821 cgagtggacc ttctcgcagg agaggtcggc ggcgccgacg agcgccgcgt cgagctcgcg
     2881 ggaacggagg gcccgcatcg cgagggagag cgccgtcgtt cccgaggcct cgcccgcctg
     2941 cacgctgaac gcgcgcccgc ccaggtcgag ctgcgagctg agccggttcg ccgggatgtt
     3001 cggcatcgtc ccgacgaccg ccgccgcctc gaggacgggg acgaccgcgt cgcgcgcgct
     3061 ccggagccac tccgcggagg cgcccgtctc gcgggccgtg cgggcgaggc gccagcgcgt
     3121 cccccaacgg gccacctcgg ggtcgggctc catcccgacg aagacgccgg tcctctcgcg
     3181 cggcagcggg ccgctcccgg cgagcgcctc cgtggcggcg cggagcgtgg cgagctgctg
     3241 cggcagggtc tgggcgaggt ccttcggagg aaagcggaga ccttcgaggt ccagctcgac
     3301 cgggccgagg cggccttcac cgctcgcgtc gaggaggctc tcgttcgaga agagcgcctc
     3361 ggcgaatcgc cgccggtccg gggcgctcgc cacggcgcag ccgagcccga cgacggcgag
     3421 cgccggaggc ggagccggaa cggcttcgcg cataccgacc cgttcgtcca gctccgccga
     3481 atcctcggag acgacgaggt gcgcgttgtt cccgccgaag ccgaacgccg agaccgcggc
     3541 gacccgcggc ccgtcggacg gccacggctc gagcttctcg acgagccgga acggcgagcc
     3601 gtcgagcgcg cgggtcggtt cctcggcatg gagcgtggga ggccgctgtc cggccttcat
     3661 cgctccgagc accttcagga gcccggcgac gccggcgacc gtgatgaggt gcccgaggtt
     3721 cgacttgaga gagccgagcg ccacgccgcc gagcccggcg aagacggcgc ccgtgctccg
     3781 gatctccgcg gcgtccccga cgggcgtccc cgtcgcgtgc gcctcgagga gggagacctg
     3841 ctcggggccg aggcccgcct cctcgtaggc ggcccggatg gcgcgctcct gcccctcctc
     3901 cgacggcgcg aggaggccct tgccgcgccc gtcgttcgag aggccgacgc cccggatgac
     3961 gccgtgaatc gtgttgccgt cccgccgggc gtcctcgagg cgtttcagga cgacgaacga
     4021 ggccccttcc gccgggacga ggccgtcggc ctcgcggtgg aaggggcgcg accggccgct
     4081 ccggctcagc gcgccgaggg ccgtgaagcc gacgtggagg aagaggtcgt cggcgcgctg
     4141 gacggccccc gcgagcatca cgtcggcctc gccgtcgtgg aggcggtcgc acgcgagctt
     4201 gatcgcgtag agcgagctcg cgcaggcggc gtcgaggcag aaggcgcccg atccgaggcc
     4261 gagcgcgcgg gagaggagcg tcgcggtccc gccgctcatg aaccggttgc gcgggtcgac
     4321 gcccgctccc tcgccggcgc gcccgttctc cgtccagacg ctccgcgcga aggcggccat
     4381 cccctccgac gggaagccga ggttgccgaa gacggcgccg gtccgagggc gggcgaccgt
     4441 gccggacctg ccgtctccag cgtctctcag cgcctcgcgc gccgtgtgga ggacccagtg
     4501 cgcgagcggg tcgagcccct cgagctccct ctcgccgacc ccgaaccccg ccgggtccca
     4561 gaccgcctcg aagcccgaga cgtagccgcc gcggtcggac caggcaccgt ccggcgccgg
     4621 gccgcgcggg tcgcgaagga cctcggactt ctcgagcccc cagcgctctt cgggcgcctc
     4681 tccgacgacg tcgcgccccg cggcgacgag ctcccagagc gcggaagggt ccagcgcgcc
     4741 gggcaggacg caggagcgcc cgacgatggc gatcggggcg aacggcatcg gctcaggcct
     4801 ggctgccggc cggatacgcg tgggtctcga ccccgaggag cgacgcgagg cgacgcccct
     4861 cgtcgtcggt gaagtggatg tccgtcgtga cgccgaggct ccccaccttg cggcggtacg
     4921 cggccacgct cacgacgccc ggcgacggag cggagcggta cgaccgcagc tcgccgatcg
     4981 tcgtcggcag gttctccttc ccgagcatcc gctgcgcgaa gaggaccgcg acctggagcc
     5041 ctccgtcgag ggccgcgacg tcgagctgcc aggggttcgc cgtgccgttc ggccatccgg
     5101 cgcgctcgac gcctctcacg cgccctccgg ccccgtggtc ggagatcccc tcgacctcct
     5161 cgatcagctc gaaagccccg cggtggaaga ggaggtcacg gtagatcggc tccccggacc
     5221 actccccgag cgggagctcg gggagctcgc tctccggggc ccggagctcg ggccgcagct
     5281 cggcccgggc gctgtagtgg tgggtccccg ccgatccgcg gacgtccagc tggagccagg
     5341 tcccggggcc gcccgaggtc tggcgggcct ggatgacgaa ccggtcgccc ccgttgtcga
     5401 atgctccgag gcggattccc ttcaggacct tgatgtcgtg cagcgcggcg agcgcgaggc
     5461 caggccggaa ggagcgggcc gcgcgagaga gccactcggc cgcgaggacc accggcacga
     5521 ccggggtccc cttgatcgag tgcccttcga gccagccgtg gctcccccgc tggaccttca
     5581 cctcgagctc ctggacccgc gcgtcggcgc ccgcgaagag gagcgcctcg gcccgcggct
     5641 ctccgccgag gacgagctcc acctccgcgc gctccgcgcc gcggagctcg tcgacgaaca
     5701 tccgtgctcc cgcgacgagc gggatcatcg ggacgccgag cgcggcgaac cgctccttca
     5761 gctgcggcgt gaccatcccg ccctcccagg gcccccaccc gagcgacttc acgaggagct
     5821 ccgggcggcg gcgggactcg gcccaggcga cctttgcgag gacctcgttc gccatggcgt
     5881 agtcggcctg gccgttgttt ccgcagcggg ccgagaccga cgagaagacg cagaggagct
     5941 tcagcggatc gctcgcggtg gcggcgagga gcgcgcggag cccctgcacc ttcgtgtcga
     6001 agacccggtc gaactgcgcg tcggtcttct gcgagatgtt ccgatccgcg aggacgcccg
     6061 cggcgtggac gaccccggcg acggggcccc actcggagcg gacgcgggcc aggaccgctt
     6121 cgagcgcctg ggcgtcctcg acgcccgcgg cctcgtatcg cgcctcgccg cccgcggctg
     6181 cgatcgcgtc gagggtccgg cggatctcgc ggttcgcgag aacctcgtgg acccgggccc
     6241 cgagctcggc cggcacgggg ctctcgccgc ggccccgggc ctcggcgagg agcgccttct
     6301 tcaggccggc ttcttccgcg atgcccgcgc agcaggcggg ctccggcgcg agcggcgtcc
     6361 tcccgaggag gacgaagcgc gcacggctct cccgcgccca ctcctcgacg cacgcggccg
     6421 tgacccctcg cgcgcctccc gagatgacga cgacatcgcc ggggacgatc accggcgcgc
     6481 cggccgtcac gggcttcgcg acgctgcgga gcgtgaagcg gcctcccgcc gccgggaggg
     6541 cgacttccgc ctcgcctccc ccttcgagga gctcggaagc aagcgccctc gcgatctcat
     6601 ccgccccgcg tccgccgcgt tcgaggtcga cggccttcag agaagcctgc ggccactcct
     6661 gccccgccgt cttcacgagc gccggcaggc ccgcgagcca ggcacgccgc gggtccatcg
     6721 gagcggtccc gaaggcggct cccgtgtcct gcaccgtcac gaagaggccg cgttccttct
     6781 cgagccgcac cgccagggtg cgcgcgaggg cgaacgcttc gcggttgacc gcgatcgcct
     6841 cgtcgacgtc cgcgacgtcc cggaggccgc caaggtagac gcaggccgtc gctccctcgg
     6901 ggagggtcga cgtcgttctc gcgttgacgc cgcgagcgcg cagctcggcc gcgagaggct
     6961 cgttcagctc cggcgacccg atgacccaga cctcgccgcc cgagaggagt ccgggctgcg
     7021 cgaggccggt ggccggggcc gctatcctct cgagagcgaa ccggcccagc gccggcgcgg
     7081 ccttcgcggc gtggaccgcc gcgggcgcgg ccggaaccgc aacgggcgcc gtcgcgggcg
     7141 ccgtcgtcgg agccgcaacg gacgccgctc ccggctgccc gacgcccttc atgtactcga
     7201 cgatggcgcc gagcgtacgg agcgacccca tgtggcccat gtccacggac cgcagctccg
     7261 gcacctggtc ggcgacggcc gagaggatct cgacccgctt gatcgagtcg atcccgaggt
     7321 ctccctcgag gtccatcccg aggttcagca tgtcggccgg gtagcccgtc ttctccgcga
     7381 cgaccgcgag catcagggcc acgaggtcga cgtcggaggt cgccgcgctc ttcgccggcg
     7441 gagcggccgg ctcctgcggc gcgagctcct tcatgtactc gacgatggcg ccgagcgtgc
     7501 ggagcgagcc catgtggccc atgtccaccg accggagctc cggcacctgg tcggcgacgg
     7561 ccgacaggat ctcgacccgc ttgatcgaat cgatcccgag gtccccctcg aggtccatcc
     7621 cgaggttcag catgtcggcc gggtagcccg tcttctccgc gacgaccgcg agcatgaggc
     7681 ccacgaggtc gacgtcggag gtcgccgccg tcggagacgc ggcggaagcg aggggcacga
     7741 cgacgggcgg cgcgggctgc gccgcgagag ccgcggttgg cggcgccggg gtctccacga
     7801 cggcagcgac cggcacgtga gccggaccgt gcacctctgc cggggccgcg gcccggacgg
     7861 tcgccggctg ctcggggacg aagcgcgaag cgggctcctt cgcgggcatc ggcgccggca
     7921 tcgcgagggg ctggcccgtc accatcgcgg tgaggccgag catccccgac tcggcgacgc
     7981 ggaggaacga ggcgtggctc tcggccatcg cccgctggta gacggcgtga gcctcggcgg
     8041 tctgccgctg cgtctcctgg aacgcgagga cccagtccga agggttcccg gccagcgcgc
     8101 gggcgggcgc ctcgtgcgac ggagacggag cggacgaaac gggcacggcg gtctcgatcc
     8161 tcatcgggct ctcctggggg gctgcggcag ggggattcgc cggggcggac gcgccctgct
     8221 gcgggagcgg cgcgggccgc ggcgggttcg gggcgggaag cgacgcggcg ccgcccggcg
     8281 ggggatagag cttgccgtgg ttgctgccgt cgatcgcgac ggcgagcttc gaggccgcgg
     8341 ccgtcccctc ggttcggtcg acgcggacgt cggcaaaggg cttcgcggga gacatcggaa
     8401 cgcccgccgc cacgaggcgc gcgagcgcga gctggagcgt ctcgacgccg ttcctcccct
     8461 tccggtcgag gttcacggcg aggtggggcc ggccgtcgag gatccgcccc aggaggcccg
     8521 tgaggaccgc gcccggcccg acctccagga acgtgcgcgc cccggcggcg tacatcgcct
     8581 cgaccatctc gacgaagcgg acctcgctcg cgacctggcc cgcgagggcg gcccgcaggg
     8641 cctcgggggc gtcctcgtgc ggggcggcga gcgcgttcga gtagaccggg agcgcgggcg
     8701 acgcgaacgg gacgttcgcg aggaaacgcg cgaatggctc gcaggcgccc gccacgaggc
     8761 tcgagtggaa cgcggtggcg accggcaggc gctggaacgt gatcccctcg gacttcagct
     8821 tcgtctcgag gtccgcgacg tcggccgtcg agcccgagac gacgacctgc gtcgggccgt
     8881 tgtggttcgc gacgacgacc cgcggggcga ggcgcgcgag aagcgcgcgg acgtccgcga
     8941 ccggcagggt cagcgccgtc atggcgcccg ggatcgcggc cgcctcggcc atcagctcgc
     9001 cgcggcgccg agccactctc aggaggtccg cgtcgctcga gaggacgccc gccgcgcgga
     9061 gcgcgacgac ctcgccgagg ctgtgccccc cggtcaggtc gggacggatc ccggcctcct
     9121 cgaggatcgc gagctgcgag agggagacgc aggcgatcgc cggctgggcc cactgcgtgg
     9181 aagtcagccg cgcgaggtcg ctcttctccc tctcctctcc gaagcccggc ctcgggaaga
     9241 cgacttcgtg gagcggcgtc cccggctcga ggtcgaggtc cgcggcccgg tcccaggcgt
     9301 cgagcgccgt ccggaacccc atcgcgaggt ccgcgcccat ccccacgtac tggctcccct
     9361 gacccgggaa gaggaaggcg accttcccct cgcacgggcc cgccccgtag gccgtgccgt
     9421 cgggcagcga gaaggcctcc ttcggggccg cctcgatccg cttcgcggct tcgaggagct
     9481 ttcttgcgag gtcgtccccg tccgcggcga gaacggcgag acgcgcggag gcgcccgggc
     9541 gatagccctc ctggctctcg cgggcgagcg cggcgaggcc ggaagccccc gcgacgccgg
     9601 cccgttcctt cgcgagctcc cgcgccttcg aagcgacctg cgccggcgtg tcgccgcaga
     9661 cgacgaccag ctccttcgcg agcgtcccga agcgcgccgc gcgcggcgcg gggccccggt
     9721 actcctcgag ggcgacgtgg aagttcgagc cgccgaagcc gaacgcgctc accgaggcgc
     9781 gccgcgggtg gcgggcgtcg cgcacccagg ggcgcgcctt cgtgctcagg tagaaggggc
     9841 tctcgggaag gccgagcttc gggttcggcg cgtcgacctt gatcgtcggg gggaggacgc
     9901 cgtggtggag cgccaggacc gccttgaaga ggcccgccgc gccggcggcg gccttcgtgt
     9961 ggccgatctg gctcttcacc gagccgagag cgcaccactg ccggtcttcc cgccccgccg
    10021 cgtcgaagac gaggcgcagc ccctcgaact cggcgacgtc gccggccttc gtcccggtcc
    10081 cgtgggcctc caggagctcg acggtctcgg ggccgtagcc cgcgagctcg tacgcgcggc
    10141 ggagcgcgag ggcctgtccc gacggcaccg gcgcatagac gctcttgctg cgtccgtccg
    10201 aggaggagcc gacgccgcgg atcacggcgt agatccggtc gccgtcgcgc tcggcgtctt
    10261 ccagtcgctt gagcgcgacc atcccgagcc cttcgcccag catcgtgccg tcggcctggt
    10321 cgctgaaggg gcggcagtcg ccgctcggcg agagcgccgg cgtcttcgag aagcagaggt
    10381 acatgaagat gtcgttcagc gtgtcgacgc cgccggagat gacgaggtcg ctgtgcccga
    10441 gctgcagctc ggcgacggcc atcgagagcg cgctcaggga cgaggcgcag gccgcgtcgc
    10501 agacgcagtt cgtcccgccc aggtcgaggc ggttcgcgat ccggcccgcc acgacgttcc
    10561 cgaggagccc cgggaacgtc gcctcctgcc acgggacgta gtggctcgcg atccggtcac
    10621 agatcgccgt cacctcgtcc tccggcaggc ccgactcgcg gagagacttc acccagacgg
    10681 gccgctgcag gcggctcacc atcgaggaga ggagctcctg ggcgctcgtc acgccgagga
    10741 tcacggagac gcgcgagcgg tctgccgtct cgaactgctc gcgcagggcg tcctgcagga
    10801 ccttttgcgc cacgacgagc gcgaggagct gggacgtgtc ggtcgccggg atcgtcgaag
    10861 ggggaacgcc ccagccgagc gcgtcgaagt cgaccttgtc gaggaacgcg ccccgcttcg
    10921 cgtaggtctt gtccggcgcg gccgggtcgg ggtcgtagta gtcctcgacg agccagtggc
    10981 ccggagggac gtccttgatc aggtccgtgc cggcgaggat gtccttccag aaaccggtgg
    11041 cgtcgctgga tccggggaag agggcgctga cgccgacgac ggcgacgggc tgccggctcg
    11101 gggagggatc gtgcttcatg ggtgcctcgg catgggacgg ttcggagctg cgagacggag
    11161 ggtcaggaaa ggggacgggg acgaaagtcg aaggcggagg gagggacggc gagccccgac
    11221 gcgcgcagct gctgcgcgcg ggtcaccgcg gcggcgccct ccaacaggtt gcgggcgacc
    11281 tggacggcgg tccggtccgc gggcttctcg aggaacgagc cgcggaccca cgagttgaac
    11341 gcgcccatcg ccggcccgca ccagatctgg aagtcggcgc tgcggtccgg ctcgcccgcg
    11401 atggcccacc ggctcgagag gccgaggtag gaacggaaga cgagcgccat gaggtggcgc
    11461 gggtcggatt cggctctcgc cacctcgcgc gggtcgcggg cggcccagaa ggcgcgcgtc
    11521 gacgcccatg cctcttcgaa cgtcgccctg aggagctgcg tctcgacctt ctcccgctcg
    11581 gcggcgggga tgctctcgag cgacgggtac gaccggtaga gctcgtagag cttcttcgcc
    11641 cggacggcga acatcgtccc gcggcgcagg acctgcaccg tgacgcccag ctcgaacatg
    11701 tcggccgcgg gcgccatgat cacgtcggcg atccccgcct cggcgaggag cgcccggccg
    11761 ctctcgtgga ggccgctctc gacgcaggcc tggttcaccg agccggtcac gacgtacgag
    11821 gcgccgaggg cgaacgccgc cgcgaccgac gagggcgttc cgaggccgcc gccggccccg
    11881 acgcgcacgg cgcgccggta tccgcgcccg gcgacgacgc ggtcgcggag gccgaggatc
    11941 gccggcagga gcgcggtcaa cggccggttg tcggtgtggc ctcccgagtc ggcctccgcg
    12001 gtgaggtcct ccgccagcgg gagcgtcgcg gcgagccgcg cctcctccgc cgtcagctgg
    12061 ccccgctcca cgagccggcg caggatctcc gccggcggag gctcgaggaa gcggatcgcc
    12121 acctcctcgc gcgagatctt cgcgaagagg tggttcttcc gccgcgcctt cccggcgggg
    12181 tccgtcgtca ggcccgtggc ggcatagcgg acgacggccg gcgtcagcgc catgtaggcc
    12241 gaggcctcga cgcgcgtcac gccgcggcgg aggtagaggt cggccacgga ctcctcgagc
    12301 tcgggctcgt tcgggctgtg gatgaggttt gcgccccagg gaaggccttt ctctccgagc
    12361 tcgttctcca gctcgtcaag cgccgcttcg acccgcgccg gagagagccc cgccgagccg
    12421 aagaagccga ggcacccggc gcgggccatc tcggagacga gcctcaccga ggcgatcccg
    12481 ttcgccatcg accccgtgac gtaggggaag cgcgtgccgt gcgcctcgca gaacgtccgg
    12541 tccccgagcc actccgggta gaggggcggc agagaggcca cgtgcgcgaa cgtcccggcc
    12601 ccgccgttcg ctgcggacgg aaccagctca cccccgaggc cgaggccgac ccggcctccg
    12661 tcgtcgcgga cgacgtgaga ggcggatcgg acctcttcga gcgcgctccc gatctcggcc
    12721 ggcgtgaagg cggcagggcg cccgttcggc cgccagcgga gcgacgaagc gggagagctc
    12781 acgaggcggt ctcggtacga aaatggcact gagtggcttt ttgcactgag gcgcggttat
    12841 atcctcatat tcgtgaaagc gccacgacct ctccgggaga ggaagaagga cgccacccgg
    12901 caggcgctgc tcgagatcgg caaccggctc ttccatcgca aggggttcga ggccaccacg
    12961 atcgacgaga tctgcgaggc ggccgggatc agccggcgga cgttcttccg ctacttcccg
    13021 agcaaggagg cgctcgtctt cccgcaccgc gccgagcgcc tggagcgctt cgtggcgttc
    13081 ctcgtgagcg ccccgccgga cgagaggccg atcgcgacgc tgcgccgcac cgcggccatc
    13141 ttcgctcgcg agtacagcga gcaccgcgcc cagctcgtcg cgcagcagaa gctcatccac
    13201 acctcgcccg ccctcctcgc ccgagagcgc gagatcgacc gggactggga gatggcgatg
    13261 tcccgcgcgt tccgcgagcg cgccggcccg ggccacgcct cggagctccg ggcccgcgtc
    13321 ctcgcgggcg cggcgatcgg cgtcatccgc gccacgatgc ggcactggtt cgcgaccgac
    13381 ggcaaggagg acctcgcccg cctcggcgag gaagccctcg cctgcctgga ccgcggcttc
    13441 tcgctcgaat agcgcgccgg ggcgcggccg ccttacgccc cgggtttcga caccttcggg
    13501 acccggcccg ccttagacgt gacggctccc gcgctgcgcg ccgaacgccg ctccccccgc
    13561 tcgcacatcg cctcgaccga cttcggcgcg gcggccgtca ggacgcggtg gccgtcctcc
    13621 gtgacgagga cgtcgtcctc gatccgcacg ccaattccga ggaacgcggg cgggaccttc
    13681 ttctcgtcgg ggcggatgta gaggcccggc tcgaccgtca ggaccatccc cggctcgaag
    13741 aggcgccact cgccggccgc gtcgtggtag cggccccggt cgtggacgtc catcccgagc
    13801 cagtgcgacg tgcgatgcat gtagaagcgg gtgtaggtcc ccttctcgag caccttcttc
    13861 cgcttccctt cgaggaggcc gagctcgagg aggccgtcga cgaggacgtc gaccgccgcg
    13921 tcgtggacgt cctggaagcg gccgcccggg cggaccttcg aggtcgccgc gtgaagggcc
    13981 gagaggacga cgcgatagac cgcctcctgc tccggcgtga agcgtccgga ggcggggaac
    14041 gtccgcgtga tgtcggccgc gtacccttcg tactcgcagc cggcgtcgat caggacgagg
    14101 tccccctcct cgatccgccg gtcgttctcg acgtagtgga gcgtcgtcgc gttcgctccg
    14161 gaggcgacga tcgtctcgta cgacggcccg gaggccccga ggcgccggaa cctcccgttg
    14221 atcgccgcct cgacctcgta ctcccaccgc cccggccgtg cgaggccccg cgcctcctcg
    14281 tgcgccgcga cggtgatcgc cgcggccttc tccatgaggg cgatctcctc gggctccttc
    14341 cgcaagcgca tctcgtggat cacgtccgtc acgtcgcaga ccgccaccgg cccacgcagc
    14401 ggcacacgcg gctcgcgccg gaagcggttc aggatcgagg tgacgaggag gtccttcctc
    14461 tcgtcgaggc cgagcgcgta gtgaagcgtc ccggcgccgc ggatcaggtc gggcagccgc
    14521 ttctcgagct cccccacctc gaatgcctcg tcggcaccgt gggccttcac ggctcccttc
    14581 acgccggccc gccgcccctc ccacgtctcc cgttcgcggt ccttcggcct cacgaagagg
    14641 cggtacaccc gcttcccggc gtccaggacg gcgaccgacc ccggctcgcc gaagcccgtc
    14701 aggtagaaga agtcggggtc ggggcggtag cggtacgtcc cgtcccagga gaggtgctgc
    14761 tcgggcgtcg cgaagaggag cgccaccccc tccttcacgc ggtcgaagaa ccgctcgcgg
    14821 cggcgggcgt aaaggtcgct cttctcgtgc ggcatggggg gaagtctacc cgcgtcggcc
    14881 ggcgcggccg aggcaggaga ggcgaacctt ccccacgact tccaagccga tctaaccgac
    14941 gaacccggtc atcgcccccc acccgctcac caccgccgac ctcttcgctc gggtcagcgc
    15001 cacatagagc agcgcccgct ccgccgtctc cgcgtttcgg cgcgcgacgg gaatcgccgc
    15061 ttcgattcgt cgttctccga tccggcccgg ccagccgctc aaacgcccgc tcctggtgac
    15121 ggcgcggccg gaatctctcg ggaagaacgt ccgggaagca ccggactccc ttctcccccg
    15181 tccggaacgg cagcttcagc gagaggtctg gcccgcggaa gaccgacctc tccctgagga
    15241 gcagatccgg gcggctctac tggaagagcg gcgtcgtgac caggaacgac gtgaggagaa
    15301 actcctcgag ccccctccgg acctgcgtgg aaaggatgga cgggatcact cgggagcgct
    15361 cggacgagcg cgtcatcctt ccgcctccag cagcttcgcc acgagccact tcagctccgc
    15421 gacgaccgta cccggttcgg gcatctcggg gacgaggcct ccgaggtcgc cctgccacgc
    15481 gtcgccgacg agggccacga ggggctccgg aaagaagtcc tcccaggtgc gaaagcccac
    15541 gcctctcacc tcgcactttg cgtgcagctg ccgccgcacg gcactcgcgt cgaagccggg
    15601 ctgcggcttt cccaggagcg tccacaggtc atagaagtcg cggcagcggg gccggaccca
    15661 ggcacgcttc tttcgccgct cctcgttctg aagcagggcg cgtagtttct cggagacgat
    15721 ctcttccagc gagtagcaga cgacgtgccc cggcagctcc tccccgtacg cctgcaggat
    15781 cggcctcgac tccgtcggca gaaggatcgg ttcgtcggcc gtaacctcca ccttcagcgc
    15841 gcagagcggc tcccgctgcc aggggaactg gattcgcacg acgaaggcct cctgcccgcc
    15901 cggatgctct tccctgagaa cgacccggtc gaccgtcgcc gtgaaaggcc cattcgcctc
    15961 catccgctcg cgggcgcgcc ctgcggccga gcggagtccg gcttcgaggt cgtcgcctcg
    16021 aggcgcgccg cggccggtga agtcgaggtc ctcgctgaac ctgtagtcgt cgaaatggag
    16081 cttccggaga gccgtgcccc ccttcatcac gagcgtcccg gcgaggcttt cctctgccgc
    16141 gatggccgag aggacgtgcc cgatggagta gtccttcagg accgtctcgt aaggcacgcc
    16201 gttctcggcc gcggccttct tcagccggag ctcgagcctc atcgccgcgc cggctccttc
    16261 aggttgtcct gcagcttcca ccgcgggatg accggcccct ttctcgcgcg cctcgggtcg
    16321 aggggctggg tcatcttcac cgggtaggcg aggagcggag cgaggacgtc ttccttcgct
    16381 ccgaacgact ccagagccca gccgagacgc ttgaccgtgg ccgccacgcc gaaccggacc
    16441 gcgtagcccg cgagcttcgg caggtcgagt gatgcggcgt gctcttcgag aatcgcgagc
    16501 gcctcgccca ggccgccgat ccgggcgggg ctcgagaagg cgtcgaggat cgtccgttcc
    16561 ctgtcggtca tcgggacccg tgttctctgg tcgatccaga cctcctcgac gccgaagaac
    16621 cgcgaaggcg tcacggtcac cagctccacc cggaggccgt cgaccgccca cacgctcgac
    16681 cgccccgccg tcggcgtccc ggacctcatc gacggcgtca cgaccttcgc cgtcgtcgtg
    16741 caggtgatcg tcctcggcac ctggtcggag aagccgtgga agtggagcgc tgtcctgtgg
    16801 ctcagggccg aaggggtcgc gagcgccacg gcgatcgcga agtcgtgcgg aggcgtcgac
    16861 ccgggcaacc gccccgtcgt ggcgtagagc cctcgccgaa tccggcgcag ccagcccgcg
    16921 agggcgagcc tggagacgag cccgttcaca cgggccggtc cgaggcccag cttctccccg
    16981 gcgcgtctgg cgtcctccga cgagaagacg agcttcccct cgtcctcgat ctcgccgagg
    17041 aacgccaggc cgtgggtctc cccgtacgga gccctgtccg gcttcgcgct acggatccgc
    17101 ctcgtatcca tagaactacg taagtttacc cgtagcagcg ttgcgcagca tggcagatat
    17161 caacgcggta cgcccggagt tcaacaggcg gccggcgtca agtcgctgat ccggaaccac
    17221 ttgccgaatc gcgaccggca gctctcgccc tgcggcgctc ttgtcgcgat gtcggcgacg
    17281 tcggagacct tcggtaccct cactccgagt cgcgcgcagc actccttcgc ggtctcgccg
    17341 acgttcttca ggagcggaac ggtccttcgc ccggttcgcg cccgggcgtg atcgcggatt
    17401 cttcgcggca cggaggggcc gatgggaaag cgcgattcga agcggcggga gatggcgaag
    17461 ctggtggcgc ggtggcgagc cagcggaaag tcagcggcct cgttcgcgag ggaagcggga
    17521 atcccggagt cgcggctgtg gtacttggcc cggcgcccgg gagcgagcca cgtggggtcc
    17581 ttcgttccgg ttcgcgtgat tccggaggag agcgcggcga cgaagcgcga gcttcgagct
    17641 ctttctcggc gccggccgac tccttcgcgt cccggcgtgg acgctcgagc ggccgaggtc
    17701 cagctcgtcc cggacccgtc ccatgtctcg cccgcaatcg ccctccgacc ctcgccctcc
    17761 gtgcgacgat ccgccgcatg gacccgatcc gcatcgtccg ccgcctcgcc gcgctctacc
    17821 ccgccgagac cgcctcacgg ctgaagcggg ccgtgatcga ggggcaggtg acggtgaacg
    17881 gcgtggtcgt cggcgatccg ggcgcgatcg tcaccgacga cgacgccgtc gtcttcgaca
    17941 cgaaccggaa ggtgcagcgg aaggtcgaca cgtcgctgac ggtcctctac gaggacgacg
    18001 acgccgtcgc ggtcgtgaag cccgccgggc tcctgacgca cccgaccgag gcgaaggaga
    18061 aggactcgct cctctcgcgc gtctcgacgt gggccgcgaa gcgcgggaag gggcggccgt
    18121 acatctccgt cgtccaccgg ctcgacaagg agacgtcggg cgtcctcgtc ttcgcgcgga
    18181 gccgtgcggg gctcgtgagc ctccaggggc agctccgggc gcacagcgtc gaccggcgct
    18241 acctcgccgt cgtggaaggg gacctcgagg gggagaaggg gacgttcgag tggccgatcc
    18301 tcgaggaggg cgggcggagg aagcgcgtcg cccgcgaggg ggagaagggg atccgcgcgg
    18361 tcaccgactg gaaggtcgtg gagcggttcg ggatcgcgac gctcgtcgag gcgcgcctgc
    18421 gcacggggcg gacgcaccag atccggatcc acttctcgca cgccgggcac ccgctcgtcg
    18481 gagaccaggt ctaccgcgac ccgaagaggc cgccgttccc cgtggcgttc cggcggcagg
    18541 cgcttcacgc cgggcacctg gggtgggaga agctcgacgg gacgaaggcc tcggtctcga
    18601 cgccggcgcc gaaggacttc gaggacctcc tggccgcgct ccggacccgc aaaggaaaag
    18661 ccgggcgtcc ggtgaaaccc ggagcgcccg gcccttcgaa gaaacgctga gcggctactc
    18721 gaggacgacg aggacgacgc ggcggttgtt cgcccgcgcc ttggagccct tgcccgtgtc
    18781 ggccgggagc gtgtcgccgt aggagatcgt cgtcatccgc tggatcggga gctggtgctt
    18841 ccgggccagg tagaggcgga cggcctcggc ccgcttggcg cccagcgcgt cgttgatctc
    18901 ggtggtcccc gtggagtccg tgtgcccctg gatctccatc cagatggggc gcttgagctc
    18961 cttgaccttc gccgcgaact cgtcgagcgc gaccttcgcg tcggcgctga gctcggcgga
    19021 gccggtgcgg aacttcaccc ggtcctcggt gaacgtctgc tcgaagacga tctgcccctt
    19081 ggcgaggatc cccgcctcct gggcgcgctt gagggcctcc tgcgcggcct gggagacctt
    19141 gacgatctcc tgcccctgcg cgtcgacctt cgtctcggtc tccttctgct tcgtctggag
    19201 ctcctcgacc tgggtctcga ccgtctcgat cttctggtcg gtcttcgccg tggcctcggc
    19261 gatcttcttg tcgacctgct tggtcgtggc gcacccgaac gcgccggtca cgaggacggc
    19321 gacgcccacg agggcagcct tgttcagctt cgtcattcgg ttcctcctca acgtcctgcg
    19381 gacgacctca cccatcgctc atttggctcc gggacggtgc ggcccgaacg cgggagggtc
    19441 ggaacccctg tatccggccc tcacgcggac ggggcgagcc ggaccggcca ccctcaatgt
    19501 gaggcggcgg tatcttaccg gaccggtgcc cgtcggtgag aacccgacaa ggtattgaga
    19561 acgaagatct ttttggatgt cgaggacctt ctcggggagg agggccgggt cgtccacgat
    19621 cgccatccgg ccgcccgtga tccgcgcgat ccgcgagagg acggcgacgt tcagcgacca
    19681 ctcgagcgtc tgcccgggcg gaggctcggc gattcccccc ggagagcgga tgccgagcgg
    19741 gaagacgggg acctcgatgc cctccatgag gctgccgagc tcctcgtcgc tcagctcgga
    19801 gtcgttgtcg atgccgtcgg agagaaggac gatcgcccgg gagccgttct tgccctggag
    19861 gctcttctcg ggcatcaggg cgagggcgtc gcggaacgcc gtcttccccc acggcttcac
    19921 ctcgtcggcc gcggcgagga tcgcccgggc gtcctccgtg aagggaacga cctccttcac
    19981 ctccccttcg gcgaagacgt agagggcgaa gtcgtcgccg ggcacgcgcg ccgccgcgag
    20041 cgcccggagc gccgcacgcg ccccttccat cttcccggcg aggcccatcg agccggagcc
    20101 gtcgaggagg atggcgtacg agaccggtgc gtcggagctc ccctggaaga ggtcgtaggc
    20161 caccggcacg ccgtctgcga ggagcgtgac gtccttctcc ttcaggccgg cgaccgggcg
    20221 ccccttgcgg tcggtgacga cgaacgggac gaggacgtac ccgaccgaga cgtcggccgc
    20281 cgcgcgcgcc ggcttcggac gcgcccgagg cacggcgggg cgcggggcgg agggtgcggc
    20341 tgcggggcgg gtcggcgagg ccttgatcgc cccggccgtc ggcgcaggcg tccggacggc
    20401 cggggcgtgc gccggcgagg ccgtcgccag aggcggctgc gccggaacgg gtgacgcggc
    20461 gtgcggcgcg acggccgtcg gcgtcggcgc cgccgtccgg accggctgga acgtgccggc
    20521 ccagggcgtc ggcgtcggcc agggggtcgg ccgcggcgac ggcatgggcg tgggcgcctg
    20581 ccgcccgaag ccgccgcgca ggcggagcgg ctcctcggcc ggcgccgaga gcgcgcccgc
    20641 gaggatcgcg acgacgaggg cgagaggtcc cttcaccggc ggattgtcgc aggccgtgtc
    20701 gaggggctcc gtccgggggc ggacgtcgga gccgcaggag accgtccgac gcgacgaggc
    20761 gtatcgttcc cggaatgggc cgccgacatc tggctacggt ctcgtcgcgg gtcgaggaag
    20821 cggacgccgc ctggtcggac cgggcgaagg ggagctccgg cggccgcgcg cgcgtcggcg
    20881 gcatccgcaa cgtcgtggcg ctgctccggg acgagctcgc cgaggagccc ctctcggtcg
    20941 cggcgcgctg gcggctcatg cgcgccctct acttcgaggg cgagtacgcc gcggcaggag
    21001 agaaggaagc gaaacggctc ttcgtcgaag ggacccgcgt cgcggacgag tgcctcgccc
    21061 tgcttcgcga ccgcgcctcc gagggctcct cgcgtgacct ctcgcgggcc acccccgtcg
    21121 agctggtgcc gcgcctcgcg ggtgaccccg acgccgcctc cgcgttcgaa tggggcggcg
    21181 tcgtctgggg catgtacgcg ctggcggagg ggatgctcgc cgctgcccgg gagggagcag
    21241 ccgggcgcat ccgcgactcc gcggaggcgg tcctccgcct cgacccgggc ctcgacgacg
    21301 cggccggcga ccggacgctc gggcggctgc accacaagac gcctgcgatc ccgttcctga
    21361 cgggatgggc ctcgcggacg gaggcgatcc gtcatctccg ccgtgccgcg agcgcggccc
    21421 cgacgagcct cgtgaaccgg cttttcctcg ccgaggcgct ccacgacctc gactcgtcgc
    21481 gccgcgacga ggccatcgcg atcgccgagg ccgtcgtgcg ggacgcgccc gacccggcct
    21541 ggctcgtcga gcacgagcgg gcccgtgccg acgcgaaggc gctcctgaag agctggcgcg
    21601 cagcgggacg ctgaggagcg ccccgccgcg cgcggacggg tcttcctaga tcagctcggc
    21661 ctgggagaag aagaacgtca cctcgcgcgc ggcgtcggcg aggtttgcgg agccgtgcga
    21721 ggcgttccgc tcgatcgact ggccgaactc cttgcggatc gtcccctcct cggcgttcgc
    21781 cgggttcgtg gcgcccatga cggcgcgcca gtgggcgacg gcgttctccc gctcgaggac
    21841 ggcgacgaag accgggcccg acgtcatgta ggcgacgagg gagccgaaga agcccttccc
    21901 cgcgtgctcg gcgtagaagc cctcggcctc ggccttcgtg atccggtgga
//
LOCUS       DAOTEZ010000013        21667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_1595_length_21667_cov_6.527531, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000013 DAOTEZ010000000
VERSION     DAOTEZ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 21667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21667
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1595_length_21667_cov_6.527531"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..101
                     /locus_tag="P5164_01305"
     CDS             3..101
                     /locus_tag="P5164_01305"
                     /inference="COORDINATES: protein motif:HMM:NF013038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HRY42556.1"
                     /translation="MRCEEWAEKLGTITYEVTCQVNPRVARVYDPY"
     gene            189..359
                     /locus_tag="P5164_01310"
     CDS             189..359
                     /locus_tag="P5164_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42557.1"
                     /translation="MADDQVTAGNIAKELGASPAQVKKAIEALKLAPVARKGACSYYS
                     REQVRKIKGALK"
     gene            376..825
                     /locus_tag="P5164_01315"
     CDS             376..825
                     /locus_tag="P5164_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42558.1"
                     /translation="MVQREHVRGPEPSPSRAGRLLFPWPPRRLPWARPVQVALRTVHV
                     AAMALVLGGLAFDVPDARLHVAGVVTVASGVGLLAVEIARTGVFLYTGAGAASLLKLA
                     LLGLGVAFPAARFELYLAATLLASIGSHMSGRWRHWSLLDRRSLTQD"
     gene            complement(850..1875)
                     /locus_tag="P5164_01320"
     CDS             complement(850..1875)
                     /locus_tag="P5164_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HRY42559.1"
                     /translation="MNRPPDDRYSRQVLFRPLGAEGQARLRAARVLVAGCGGLGAESA
                     SLLARAGVGFLRIVDRDVVELSNLQRQALFDEADVREGLPKAVAAARRLARVNSDVAV
                     EPIVADIGPRNVRGLLDGVDLVVDGFDSFEARFLMNDACVETRTPLVYGACIASTGLA
                     ALLVPGVTPCLRCLLPELPEPGSAATCDTAGILGPAAHLAASMQVGRALRYLSTGESP
                     PEAELVYADVWEARFTRMAVPRDPACPCCAGRQFDFLSVETPQTAVLCGRDSVQVRPL
                     VPSKPDFATLAERLRTLGEVVANDWLLRFRTEDAELTLFSDGRAVVKGTNDPARARSL
                     VARTFGV"
     gene            2080..4221
                     /locus_tag="P5164_01325"
     CDS             2080..4221
                     /locus_tag="P5164_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HRY42560.1"
                     /translation="MKAAHRVLLEVPFTPSEPERGYNMRSLYVDLTTLTVKEKQVTPQ
                     MKDLFVGGRGFGLWYLWNATTPTTRWNDPENEIVIAPGPIAGITQYPGTGKSLVVSIS
                     PLTDIPIDCNVGGYYGPLLKFCGFDAIEIQGKADREVVIVVDGPRGVIRIEEAPLEPP
                     DSHVLSEIFTHMYATGPEDYRNVSVVSAGKGSDHSYIGCLNFSWWDPKRKAARLKQAG
                     RGGVGTVFRDKRLKALVVRGPVIKADLNHVADRARIQKVGSVINKEIHDLDAQQCNMR
                     RQGTAHLVEVMDAYDLLPVKNFQYGNHPDTKNIASWVWDKRFTQGIPDGCWYGCTMSC
                     SKGADGHVVKTGPYKGDVVTVDGPEYENAAGLAANCGIFDPDWLLESNFYCDTYGVDT
                     ISYGTLCAFVMECWQRGILNAERTGGLDLTWGNAEAQMEMLHQMARGEGFGVIAGKGI
                     RQMKRIFAENGWGDPKLMNDIGMEVKGLEYSQYQSKESLAQQGGYALTNKGPQHDEAW
                     LIFMDMVNKHLPTFEDKAEALHYFPLFRTWFGLNGLCKLPWNDIEPANNAEQPEPAKV
                     PEHVHNYCEIASGVTGIEVTKEKIIEQSEKVYNFQRVFNLRRGYGKREHDWPPYRSVG
                     PVTVEEYESRAERYDKQMREEIGVDPEGRTTEEKIAITRKYREDRYAKLCDAVYKRRG
                     WTTDGVPTVETLKKLGIDLPELVELVKQHGG"
     gene            4226..4471
                     /gene="thiS"
                     /locus_tag="P5164_01330"
     CDS             4226..4471
                     /gene="thiS"
                     /locus_tag="P5164_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HRY42561.1"
                     /translation="MDPKPGAPGAEADRGTRLVVNGDPLDWFEGMTVRDVLRAKNYVF
                     KLIIVTVNGALVPRGSYDEAPVPAGADVKVVHLISGG"
     gene            4584..5699
                     /locus_tag="P5164_01335"
     CDS             4584..5699
                     /locus_tag="P5164_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42562.1"
                     /translation="MSRPKLLPAAFSLAVLLAAFVAFPAGGEGAADGIALTGSVKKSQ
                     LLDAASLAGLPRQRLHVKLSGPGGVYRATVEADAVALRELLDRAEVAKSMDDNFDRPL
                     DLAVVVTGRDGTKAVFSWGELFLAGDSGAAVLADRLRPFMPHHHEPVADAVFASGAWL
                     GPDARASLDVSSCTACHDGGKLSKIDVPRGTCLVPTRDATGRRFVEDVVSIEVRQAGF
                     PAPPRKKDLKNPWVEAPRLVLPNGKSTPLSAKATRSLPRVAGEAEAIGLGRGYRGRSR
                     FAGASLAALLRQALPAGTDPGLLFVVVTGSDGYRSLYSGGELLLSHLPENGVLVDTEG
                     GKPLLRKSGRLKSVVRGDFFVDRSVRNVSEIRILTVP"
     gene            complement(5727..6956)
                     /locus_tag="P5164_01340"
     CDS             complement(5727..6956)
                     /locus_tag="P5164_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005031626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HRY42563.1"
                     /translation="MTTCSDALRLILDSVVPLPARPTPLFDALGLVLAERVISPETVP
                     PFTNSAMDGYAVRADDLSSAMAASPALLDVLADLPAGSVATHEVAPGTAIRIMTGAPL
                     PADADSVVPVEATTSEGSRVRITAPVKRGANVRLAGEDLREGEELLPSGFALTPAALG
                     ILASVGLAQAPVRPRVRVAILTTGDELVDASARPGPGQIRDSNVQTMCGQAAAFGAVP
                     IPIPRVPDTREAVTAALNRARAEADVVVTNGGISVGDYDFIKAVLGDFGAREVFWKVK
                     QKPGGPLGFWLWDGTPVFGIPGNTVAAMVCMEEYVRPALRKMMGHGRLLRPEVTAVFE
                     GEFRKSAPDGKVHLIRVRARREGDRVVVSPSGPQGSGILSSMLRSNALALVPADALAL
                     RAGDEVLVHLIEEPEDR"
     gene            complement(6943..8052)
                     /locus_tag="P5164_01345"
     CDS             complement(6943..8052)
                     /locus_tag="P5164_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY42564.1"
                     /translation="MSLLALAGVEVARDGKAILSLEGLDVRAGELLALLGPTGAGKST
                     LLRVLHLLERPAAGTVAWRGAPVPWPAPLDLRRRIAMAFQDPLLFSGSTLENVLYGLS
                     LRGETGVAAREKATEALRLLHVDHLAERRARTLSGGEAQRTALARALVLRPELLLLDE
                     PFAALDAPIRRHLLEELTQVVKAHGITCVYVTHEQGEAFSVADRIAVLRRGRLLQAGT
                     PEEVLLRPASRAVARFMQTGNILSGVVTRRSEALSEVAVGACVLFSRTVLPEGTTVDA
                     CVRREEVLVEAPGDAAPAPGLNRFRGTVATVFDHGSTMQLKIDAGFSEGPLQALITRR
                     VAHDLAPRPGLPVEATFSAASVHLIPREGRSDDDL"
     gene            complement(8049..8732)
                     /locus_tag="P5164_01350"
     CDS             complement(8049..8732)
                     /locus_tag="P5164_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY42565.1"
                     /translation="MTAPLASLGALLPAPGELAGIILLSLFVSGVAIVVSMLVGVPAG
                     IFLGLHRFPGRSFLVTLVNTGMGLPPVVVGLGVFLLLARSGPFGGLDLLYTPWAMVLA
                     QVVIATPLVVGITLAAIQGLDERLHLQILSLGASRAQLYLKLVKEARLSLVAALAAGF
                     GSIISEVGAVMMVGGNIKGQTRVMTTAIVLETRQGEFANAIVLGVILLALALGINWLF
                     TAVQQRRRA"
     gene            complement(8729..9550)
                     /locus_tag="P5164_01355"
     CDS             complement(8729..9550)
                     /locus_tag="P5164_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRY42566.1"
                     /translation="MHRKILSLAAVLAALVASVPRAEAQGSLILATTTSTQDSGLLDD
                     LLPRFEKATGIRVKTIAVGSGEALAMGRRGDADVLLVHSKAAEDEFLAGGFGVLRLDV
                     MYNDFILLGPPPDPAGVRGLRALDAMRRLAEKGALFASRDDRSGTHARELELWKQAGV
                     DPTGQSWYVATGQGMGETARIASEKRAYTLADRGTYLSLRRTLDLAVLVEGDATLRNS
                     YRVLVVSPVKHPQVRAAAARRFAEWLVSEEAQRAIGAFGRERFGQPLFVPDAKKG"
     gene            complement(9677..10900)
                     /locus_tag="P5164_01360"
     CDS             complement(9677..10900)
                     /locus_tag="P5164_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRY42567.1"
                     /translation="MTTRFARRMEALKASEIREILKVTQRPEVISFAGGLPAPELFPV
                     RRLAAATQEVFEEEGTRALQYSTTEGHPPLRRKIAERMNASWGAQVTPEQVLVTSGSQ
                     QGLDLTGKLFLDEGDVVLCESPTYLGALSAWNVMRPRWVEVATDDDGMDPAALESKLR
                     AHPNAKAIYVIPTFQNPSGRTWSLERRRRFMEVVERHDVWVVEDNPYGELRLEGESVP
                     SLKSFDTKGRVVALGTFSKVFCPGLRIAWVAADLSVIEKLVILKQGADLQTATLSQLQ
                     VNRYLEENDLDADIAKIIPVYRERRDAMLSALEQEMPPGVRWTRPQGGLFLWMTLPAN
                     LNARTLLDECVRQNVAFVPGGAFFPNGGNENTLRLNYSNMPIERIREGVKRLAASVAK
                     EMERAGVLEEVPATP"
     gene            complement(10953..12461)
                     /locus_tag="P5164_01365"
     CDS             complement(10953..12461)
                     /locus_tag="P5164_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRY42568.1"
                     /translation="MDIRIDRQSAVPVYLQIRGGIRDLILSGRLPDGFRLPPERTLAK
                     ALGVNRTTILSAYRELKTDGLVEAHVGRGTEVASQQVLRSEAEPAGPLPWRQLARDGG
                     VREPDPLVRDVLALTERHDVISLQAGLPAAELLPLAELRRVQEEILVRKGPEALLHSP
                     TEGVTAFREALCRHMVPRGIRTSPEEVLVTSGSQQGLDLVARTLLSPGDLVVVEEPSY
                     FGALEAFRSARVRLLGVPVDAEGMRTDILEALLARQKPRLVYTLPTFQNPSGAVLSPA
                     RRRHLLELSWRHQVPVLEDDTYSDLRYEGSPLPSLKALDPYGHVIHLSSFSKVLFPGL
                     RLGWIAAPRPLVRQLALVKQTVDLHSGTLGQWIVEGFLSSGLLSAHLARVRAAYRRRR
                     DVLLEALAAEAVPGLSWSRPEGGFYLWCRLPDDVSPASLVAHAAAERVAYLPGAASFV
                     EEPSESSIRLNFTFAPEDDLREGARRLGSALRAAQAEPRRPSRAVVGTPPIV"
     gene            complement(12757..14715)
                     /locus_tag="P5164_01370"
     CDS             complement(12757..14715)
                     /locus_tag="P5164_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRY42569.1"
                     /translation="MPASRRFSPLAALLLALPALGEPQTAYKGHGADSVPAEVIARYA
                     PKPLPPDLARRIQSMMDVRAPGMGIVSPDGSKLFFGWSVTGTSQVWRLDGPNTFPVQL
                     TGGEDRTTVVDVFPDGKTLVLQRDRKGEENPGLYLMPAAGGPLTSIQHLPGIQTFHQF
                     VSPDGAWVYFASNDVKPDSYAVYRWNVATRQREALVTEPGLWSVSDHRPDGTLLLAKW
                     TGSLTSEYWEWSPSTRKLTPLLGQDRPEEYTAVYGAAPGQLLVLTPKLGEFRRLCRWE
                     KGALTPLTGDLSWDVSGFSLDEAKTRVLYTVNEAGYTKLFVLDAKTFQPVRLPKLPEG
                     ADHVYAGASTRDGRFTTFGVETATAPRTSYVYDWQSGALTRWVVPSAPEIDTTTFAAA
                     TLEHYPARDGTKIPAFVRRPKSCDPAPCPVVVEFHGGPEGQAQPGFSTWAQAFVDAGF
                     VFVEPNVRGSDGYGRTWLDADNGRKRLDVWTDIEDAATWARKAFAANGKEPRVGVTGG
                     SYGGYATLVAMTKFAGAYDAGASIVGISNLLTFLENTAPYRRILRTTEYGDPEKDREA
                     LLELSATTHLAKLKAPLQIQQGATDPRVPVGEAIQMYEAAQKTGVPTELIVYADEGHG
                     AGKRDNQVLMLGHALRFLETHLKGTPAK"
     gene            complement(14874..16838)
                     /locus_tag="P5164_01375"
     CDS             complement(14874..16838)
                     /locus_tag="P5164_01375"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015201193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRY42570.1"
                     /translation="MNRARRIAALAAALALLAAPAAAQQTAYQGHGADSVPPEVLAKY
                     VAPPLPSELSRKIQNLMDVRAPSGGRLSPDGARLYFGWAVTGTAQVWRLDGPNTFPIQ
                     MTGGEDRTSLADVTPDGKTLILSRDRKGEENPGLYLMPADGGPMKVVQHLPKVQTSFA
                     FVSEDGKWLYFRSNDVKPNSYAIYRYELATGKKETVFDEPGLWFVADHKPDGRILLVK
                     ATGSMTFEYWEWDPAKKAKAPMLGTEKPGRYTAQYGPKEGQLVVVTPEFGEFQRGYLF
                     EAGKWTPITPELPWDVDRAGMDEKKTRVLYTVNEGGYSKVFGLDAKTWKPLEMPKLPA
                     ADHATFGSSTPDGRFTSFAVGTPTAPSQSWVYDWREKKLVQWVVPSAPEVDTKSFAVA
                     TLEHYPARDGTKIPAFVRRPKSCDPAPCPVVVLFHGGPEGQATPGFSPMAQLWVDEGY
                     VLVEPNVRGSEGYGKTWLDADNGPKRLDVLSDIEDAALWARKAFAVDGKAPKLGVMGG
                     SYGGYATLIAMTKYAGAYDAGVSNVGISSLVSFLQNTAPYRRILRASEYGDLEKDREA
                     LEKLSAVTYIDRLKDPLLLIQGATDPRVPVGEAIQMYEATTKRGVPTGLIIFPDEGHG
                     AQKRENRVLQTGHTIQWFDRHLKGKGADAK"
     gene            complement(16952..17428)
                     /locus_tag="P5164_01380"
     CDS             complement(16952..17428)
                     /locus_tag="P5164_01380"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRY42571.1"
                     /translation="MTDTPAFQDLYPDHWALCYGCGRLNEHGLKIRSFWDGDETVATI
                     VPRPHHTSVPGFVYGGFIASVIDCHSTGSAAAAAHRAAGGDPAVGPYPRFLTGTLKVE
                     YLKPTPIDAPVELRSTIVEVKERKVVLATSLFSKGVLCARGEAILVKVPETWTPKT"
     gene            complement(17425..18012)
                     /locus_tag="P5164_01385"
     CDS             complement(17425..18012)
                     /locus_tag="P5164_01385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015149421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRY42572.1"
                     /translation="MSDAPRILALAGSLRAGSYNRRVLAVAADGARAAGADVTGIDLR
                     DFPLPVYDADLESASGLSAAALALKERFRAAEGLLIASPEYNAAISGTLKNVLDWVSR
                     PAPGEKPLACFKGKVAALCSASTGALGGIRGLASVRVVLGNLGVVLLPDQVALPKAAE
                     AFDADGNVADPARRKSLMELGAALARATRALRSPS"
     gene            complement(18054..19556)
                     /locus_tag="P5164_01390"
     CDS             complement(18054..19556)
                     /locus_tag="P5164_01390"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRY42573.1"
                     /translation="MKLESFRRRFFELLPRYEWEGTLGRGGIGVVFRALDRELGESVA
                     IKVLSPNLARDENALLARFKREVALNRKIKHPNVARMFDYGIAGDYPFITMELIEGED
                     LWTLIGSRQRFSAAEAVPIVRQIARGVAAIHRLGILHRDLKSQNVLVDRHGAAVILDF
                     GLARGSFDPGLTLASVLLGTPEYMSPEQALGDPLDERADLYSIGVIAWEMLSGQLPFA
                     GDSPVATAMKHVHEPLPDLRELAPGLPADLFAVLMRVLAKKREDRFRSAQELDDALSL
                     CDTGPASPLPEERLLPEAEAFLREVEVAIDSIILPREARPASRGDAPKTLVSTRTRRR
                     PPVVLVVEEDVRGLLKLATTLCMTGCRTLEVRSAEEALETALSRQVDLVLMSTRLPGM
                     DGFDVARILKSIPRAAHIPVLLLAPGPDRAKHAFALQSGAADLLPEPVPQALLEERTW
                     DLLRRRGFSRPSESGPAREKAPPSRPPSRPGSRPSPRPSPRSRERTRSRS"
     gene            complement(19576..21387)
                     /locus_tag="P5164_01395"
     CDS             complement(19576..21387)
                     /locus_tag="P5164_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42574.1"
                     /translation="MRHRAALPRGATSRLRAVLAAVVVAAAGCGGGDTMPAPLPSGTP
                     RPLGGASALELFPALSPSGDELASSVMTNGKLEIHVRSLSGGPGRPLTRDGRNNVQPA
                     WSPDGRLIAFHSKDRGGIWIVPASGGSPRLLASFGSRPSFSPDGRRVAFQSTPLTDVA
                     ATAAPGLAPSTIWVVDAAGGAALAVTNPGSPIGGHGAPAWSPDGDRIYFVTSEPRLTQ
                     AEIWSVKPDGSDLVRLHAGTRIYGVCVPRGAKALWFGGQGKASLYALHRISLDERGRT
                     AVGPAEAMTPPGSAVPHSPSFSADGSRLAWSELTTTAAIWSLPLDPATSLSAGSPSPL
                     TGGDGRATWPVFSPDGSTVAFGWVPPGGTNDVWLMEPDGASQRPVVENPSIDYASDFF
                     PDGRRLLFFSNREGGTFAPWELEIATRTFRRIPADLHDAIALRLSPDGKRIAYHTKRD
                     SITINAWVVDLATRTSQQLTFDREFVGYPCWSPDGRELALEVRRGDDIFIGVIPSAGG
                     TPELLTQKRGLSWAFSYSPDGRRIAFAGYRDDTWNLWWIDRNTREERRLTENLRLDVY
                     IRYPSWSPKGDRILYEQAETRGEVFLLDGRATSAAPR"
BASE COUNT         3193 a         7270 c         8201 g         3003 t
ORIGIN      
        1 agatccgctg cgaggagtgg gccgagaagc tcggcacgat cacctacgag gtgacctgtc
       61 aggtgaaccc gcgggtggcc cgggtctacg acccgtactg acggggcgcc gtccgcgccc
      121 ttccgtcctt gcgccggtcc cgtctatcct gtccgaccgg aacgcacggg ccacgggagg
      181 agaacgagat ggccgacgac caggtcaccg cggggaacat cgcgaaggag ctgggggcct
      241 ccccggcgca ggtgaagaag gcgatcgagg cgctgaagct cgcgcccgtc gcgaggaagg
      301 gcgcctgcag ctactactcg cgcgagcagg tgaggaagat caagggcgcg ctgaagtagc
      361 cggatcccgc cgcccatggt ccagcgggag cacgttcgcg gcccggaacc gagcccgtcg
      421 agagccggcc ggctcctctt cccctggcct ccgcggcggc tgccgtgggc gcgcccggtc
      481 caggtcgcgc tccgaacggt ccacgtcgcg gcgatggctc tcgtcctcgg cggcctcgcg
      541 ttcgacgtcc ccgacgcgcg gctccacgtg gccggcgtcg tcaccgtcgc gagcggtgtc
      601 ggcctgctcg ccgtcgaaat cgcgcgaacg ggcgtcttcc tctacacggg ggcgggcgcc
      661 gcctccctcc tcaagctcgc ccttctcggc ctcggagtcg cgtttcccgc ggcgcgtttc
      721 gagctctacc tcgcggcgac gctcctcgcc tcgatcggct cgcacatgag cgggcggtgg
      781 cgacactggt cgctgctcga ccggagatcg ctgacgcagg actgaccggg ggcgggcggc
      841 gacgtcccgc tacaccccga aagtgcgtgc cacgaggctc cgggcccgcg ccgggtcgtt
      901 cgtccccttc acgaccgctc ggccgtcaga gaagagggtc agctccgcgt cctccgttcg
      961 gaagcgaagg agccagtcgt tcgcgacgac ctcgccgagg gtacggagcc tctcggcgag
     1021 cgtggcgaag tcgggtttcg aagggacgag cgggcgaacc tgaacggagt cgcgcccgca
     1081 gaggacggcg gtctgcggcg tctcgaccga gaggaagtcg aactgccggc cggcgcagca
     1141 ggggcaggcc gggtcgcgcg ggacggccat ccgggtgaag cgggcctccc agacgtcggc
     1201 gtagacgagc tcggcctcag gaggggattc tcccgtcgag aggtagcgga gcgcgcggcc
     1261 gacctgcatc gaggccgcga ggtgcgccgc ggggccgagg atgccggccg tgtcgcaggt
     1321 ggcggccgag cccggctcgg ggagctcggg gagaaggcag cgaaggcacg gcgtgacgcc
     1381 cgggacgagg agcgccgcga ggccggtcga ggcgatgcag gcgccgtaga cgagaggggt
     1441 gcgcgtctcg acgcacgcgt cgttcatcag gaagcgggcc tcgaagctgt cgaagccgtc
     1501 gacgacgagg tcgacgccgt cgaggaggcc ccggacgttc ctcggtccga tgtcggcgac
     1561 gatcggctcg acggcgacgt ccgagttgac gcgggcgagc cgccgcgcgg cggcgaccgc
     1621 cttcgggagc ccctcgcgga cgtcggcctc gtcgaagaga gcctggcgct gtaggttcga
     1681 cagctcgacg acgtcgcgat cgacgatccg caagaagccg acgcctgccc gggcgaggag
     1741 gctcgccgac tcggccccga gcccgccgca gcctgcgacg aggacgcggg cggcccggag
     1801 gcgcgcctgc ccttcggcac cgagcgggcg gaagaggacc tggcgggagt accggtcgtc
     1861 gggagggcgg ttcatcgcga tcgattctct cgcgaagggg cgttccgaat tccggacctt
     1921 tcttgccccg ctttgggtcc gagtgctacc gttccaaggg cggttggccc cacccgacgc
     1981 cccgaccggg gcgcgccggc cggccgcgac gaggcgagca tgtcgacgac gacgcctgac
     2041 tacaccgtcc gcacgagcta caccgacgac ctcgagacga tgaaggcggc ccacagggtc
     2101 ctcctcgagg tgccgttcac tccgagcgag ccggagcgcg gctacaacat gaggtccctc
     2161 tacgtcgacc tgacgaccct cacggtgaag gagaagcagg tcacgccgca gatgaaggac
     2221 ctcttcgtcg gcgggcgcgg cttcggcctc tggtacctct ggaacgcgac gacgcccacg
     2281 acacgctgga acgacccgga gaacgagatc gtcatcgccc ccgggccgat cgcggggatc
     2341 acgcagtacc ccggcaccgg caagtcgctc gtcgtctcga tctcgcccct caccgacatc
     2401 ccgatcgact gcaacgtcgg cggctactac gggccgctcc tgaagttctg cggcttcgac
     2461 gcgatcgaga tccaggggaa ggcggaccgc gaggtcgtga tcgtcgtcga cgggccgcgg
     2521 ggcgtcatcc ggatcgagga agcgccgctc gagccgcccg acagtcacgt cctctccgag
     2581 atcttcacgc acatgtacgc gaccgggccc gaggactacc ggaacgtctc cgtcgtctcg
     2641 gcggggaagg gctcggacca ctcctacatc ggctgcctga acttctcctg gtgggacccg
     2701 aagcggaagg cggcgcgcct gaagcaggcg gggcgcggcg gcgtcgggac ggtcttccgc
     2761 gacaagaggc tcaaggccct cgtcgtccgc gggccggtca tcaaggccga cctgaaccac
     2821 gtcgccgacc gggctcggat ccagaaggtc gggagcgtca tcaacaagga gatccacgac
     2881 ctcgacgccc agcagtgcaa catgcggcgc caggggacgg cccacctcgt cgaggtgatg
     2941 gacgcctacg acctcctccc ggtcaagaac ttccagtacg gcaaccaccc cgacacgaag
     3001 aacatcgcct cgtgggtctg ggacaagcgc ttcacgcagg ggatccccga cggctgctgg
     3061 tacggctgca cgatgtcgtg ctcgaagggg gccgacgggc acgtcgtgaa gacgggaccg
     3121 tacaagggcg acgtcgtcac cgtcgacggc cccgagtacg agaacgccgc cggcctcgcc
     3181 gcgaactgcg gcatcttcga ccccgactgg ctcctcgagt cgaacttcta ctgcgacacc
     3241 tacggcgtcg acacgatctc ctacggaacg ctctgcgcgt tcgtcatgga gtgctggcag
     3301 cgcggcatcc tgaacgcgga gcggacgggc ggcctcgacc tgacctgggg gaacgccgag
     3361 gcgcagatgg agatgctcca ccagatggcg cgcggcgagg gcttcggcgt catcgcgggg
     3421 aaggggatcc gccagatgaa gcggatcttc gcggagaacg gctggggcga cccgaagctg
     3481 atgaacgaca tcggcatgga ggtcaagggg ctcgagtact cgcagtacca gtcgaaggag
     3541 tcgctcgcgc agcagggggg ctacgccctg acgaacaagg gaccccagca cgacgaggcc
     3601 tggctcatct tcatggacat ggtgaacaag cacctcccga ccttcgagga caaggcggag
     3661 gcgcttcatt acttcccgct cttccggacg tggttcggcc tgaacgggct ctgcaagctc
     3721 ccctggaacg acatcgagcc ggcgaacaac gccgagcagc ccgagccggc caaggtgccc
     3781 gagcacgtcc acaactactg cgagatcgcc tccggcgtga ccggcatcga ggtcacgaag
     3841 gagaagatca tcgagcagtc ggagaaggtc tacaacttcc agagggtctt caacctgagg
     3901 cgcgggtacg ggaagcgcga gcacgactgg ccgccgtacc gctccgtcgg cccggtgacg
     3961 gtcgaggagt acgagtcgcg ggccgagcgc tacgacaagc agatgcgcga ggagatcggc
     4021 gtcgacccgg aggggaggac gaccgaggag aagatcgcga tcacgcggaa gtaccgcgag
     4081 gaccgctacg cgaagctctg cgacgccgtc tacaagcgcc gcggctggac gacggacggc
     4141 gtcccgaccg tcgagacgct gaagaagctc ggcatcgacc tccccgagct cgtcgagctc
     4201 gtgaagcagc acgggggctg agccggtgga cccgaagccg ggcgccccgg gggccgaggc
     4261 ggaccggggc acccggctcg tcgtgaacgg cgacccgctc gactggttcg aggggatgac
     4321 cgtccgcgac gtgctgaggg cgaagaacta cgtcttcaag ctcatcatcg tcaccgtgaa
     4381 cggtgcgctc gtcccgcgcg ggtcgtacga cgaggcgccg gtccccgccg gcgccgacgt
     4441 caaggtcgtc cacctcatca gcgggggata ggggtcaggc gtgaaatcgt gtgacgtcga
     4501 cgacaataca cgatttcacg cctgaccccg tcgatccgta cgcgaccggc cgcgaggcgg
     4561 tgtcgtatcg aaggaggaac cccgtgagcc gtccgaaact gctccccgcc gccttctcgc
     4621 tcgccgttct cctcgccgcg ttcgtggcgt tccccgccgg gggggagggc gccgcggacg
     4681 ggattgcgct gaccggctcg gtgaagaagt cgcagctcct cgacgccgcg tcgctcgccg
     4741 gtctcccgcg gcagaggctc cacgtgaagc tgtcggggcc ggggggcgtc taccgggcga
     4801 cggtcgaggc cgacgcggtc gcgctgaggg agctcctcga tagggccgag gtcgccaagt
     4861 cgatggacga caacttcgac cgaccgctcg acctggccgt cgtcgtgacg ggccgcgacg
     4921 ggacgaaggc cgtcttctcc tggggcgagc tcttcctcgc gggcgactcc ggcgcggcgg
     4981 tcctcgcgga ccggctccgt ccgttcatgc cgcaccacca cgagcccgtc gcggacgcgg
     5041 tcttcgcctc cggggcctgg ctcggccccg acgcgcgcgc gagcctcgac gtgtcgagct
     5101 gcaccgcgtg ccacgacgga gggaagctct cgaagatcga cgtcccgcgc gggacctgcc
     5161 tcgtcccgac tcgcgacgcg acgggacggc gcttcgtcga ggacgtcgtc tcgatcgagg
     5221 tgaggcaggc gggcttcccc gcgccgccga ggaagaagga cctgaagaac ccgtgggtcg
     5281 aggcgccgcg cctcgtcctt ccgaacggga agtcgacacc cctgtccgcg aaggcgacga
     5341 ggtcgcttcc gcgcgtcgcc ggcgaggccg aggcgatcgg cctcgggcgc ggctaccgcg
     5401 ggcggagccg gtttgcggga gcgagcctcg cggcgctcct gcgacaggcg ctgcctgccg
     5461 ggaccgaccc cggtctcctc ttcgtcgtcg tcacggggag cgacggctac cggtcgctct
     5521 attccggcgg ggagctcctc ctgtcgcacc tcccggagaa cggcgtcctc gtcgacaccg
     5581 agggcggcaa gcccctcctt cggaagtcgg ggcgcctgaa gtccgtcgtg cgcggcgact
     5641 tcttcgtcga ccgctcggtg aggaacgtct ccgagatccg aatcctgacc gtgccctgac
     5701 ggccccggct tcgaccccgg tctctctcag cggtcttcgg gctcctcgat gaggtggacg
     5761 aggacctcgt cgcccgccct cagggcgagg gcgtcggcgg ggacgagggc gagggcgttc
     5821 gaccggagca tcgacgagag gatgccggag ccctgggggc cggaggggga gacgacgacg
     5881 cgatcgccct cgcggcgggc gcgaacgcgg atgaggtgga ccttgccgtc cggggcgctc
     5941 ttccggaact ccccctcgaa gacggccgtc acctcgggcc ggaggagccg gccgtgcccc
     6001 atcatcttcc ggagcgcggg cctgacgtac tcctccatgc agaccattgc ggcgacggtg
     6061 tttccgggga tgccgaagac gggcgttccg tcccagagcc agaagccgag cgggccgccc
     6121 ggcttctgct tcactttcca gaagacctcg cgcgccccga agtcgccgag gaccgccttg
     6181 atgaagtcgt agtcgccgac ggagatcccg ccgttcgtca cgacgacgtc ggcctcggca
     6241 cgggcgcggt tcagcgcggc ggtcaccgcc tcgcgcgtgt cggggacgcg cgggatcggg
     6301 atgggtaccg cgccgaacgc cgcggcctgc ccgcacatcg tctggacgtt cgagtcgcgg
     6361 atctgtcccg gaccgggcct cgccgacgcg tcgacgagct cgtcgcccgt cgtcaggatc
     6421 gcgacgcgga cgcgcgggcg gacgggcgcc tgggcgagcc cgacggaggc gagtatcccg
     6481 agcgccgcgg gcgtgagggc gaagccggac gggaggagct cctcgccctc gcggaggtcc
     6541 tctccggcga ggcggacgtt cgcgccccgc ttcacggggg ccgtgatcct cacgcggctc
     6601 ccttccgaag tcgtcgcctc gacggggacg acgctgtcgg cgtcggccgg gagcggcgcg
     6661 cccgtcatga tccggatcgc ggtcccgggc gccacctcgt gcgtcgcgac gctcccggcg
     6721 gggaggtcgg cgaggacgtc gaggagggcc ggagacgcgg ccatcgcgga ggaaaggtcg
     6781 tcggcgcgga cggcgtagcc gtccatcgcc gagttcgtga acggcggaac ggtctccggc
     6841 gagatgaccc tctcggcgag gacgagaccg agggcgtcga agagcggcgt gggccgcgcg
     6901 ggaagcggga cgacggagtc gaggatgagg cggagcgcgt cgctacaggt cgtcatcgga
     6961 tctcccttcg cgggggatga ggtggacgga cgcggcggag aacgtcgcct cgacgggcag
     7021 gccgggtctc ggggcgaggt cgtgggcgac gcgccgcgtg atgagggcct ggagcggtcc
     7081 ctcggagaag ccggcgtcga tcttcagctg catcgtggag ccgtggtcga agacggtcgc
     7141 gacggtgccg cggaagcggt tgagcccggg cgccggggcg gcgtctccgg gcgcttcgac
     7201 gaggacctcc tcgcgcctca cgcaggcgtc gacggtggtc ccttcgggaa ggaccgtgcg
     7261 ggagaagagg acgcacgctc cgacggccac ctcggaaagg gcctcgctcc ggcgcgtcac
     7321 gacgccggag aggatgttcc cggtctgcat gaagcgggcg acggcgcggc tcgccggccg
     7381 gaggaggacc tcctcgggcg tccccgcctg gaggagcctc ccgcggcgca ggacggcgat
     7441 ccggtcggcg acggagaagg cctccccctg ctcgtgcgtc acgtagacgc aggtgatccc
     7501 gtgcgccttc acgacctgcg tcagctcctc gaggaggtgc ctgcggatcg gcgcgtcgag
     7561 cgcggcgaac ggctcgtcga ggaggaggag ctccggccgg aggacgagag cccgggcgag
     7621 cgcggtgcgc tgcgcctcgc cccccgagag cgtccgcgcc cgccgctcgg cgaggtggtc
     7681 gacgtggagg agccggaggg cctccgtcgc cttctcgcgg gcggcgaccc ccgtttcgcc
     7741 gcggagcgag aggccgtaca ggacgttctc gagcgtcgaa ccggagaaga ggagcgggtc
     7801 ctggaaggcc atcgcgatcc ggcggcgcag gtcgagcggc gccggccagg gaaccggcgc
     7861 gccgcgccag gcgaccgtgc cggcggcggg cctctcgagg agatggagga cacgcaggag
     7921 cgtgctcttg ccggcgcccg tcgggccgag gagggcgagg agctcccccg cgcgcacgtc
     7981 gagcccctcg agcgagagga tcgccttccc gtcgcgggcg acctcgacgc cggcgagcgc
     8041 caggaggctc acgcccggcg cctctgctgg accgcggtga agagccagtt gatgccgagg
     8101 gcgagcgcca ggaggatgac gccgaggacg atcgcgttcg cgaactcccc ctgtcgcgtc
     8161 tcgaggacga tcgccgtcgt catgacgcgc gtctgcccct tgatgttccc gccgaccatc
     8221 atcacggcgc cgacctccga gatgatcgac ccgaatccgg cggcgagcgc ggcgacgaga
     8281 gagagccgcg cctccttcac gagcttcagg tagagctgcg cccgggaggc gccgagcgag
     8341 aggatctgga ggtggaggcg ctcgtcgagc ccctggatcg ccgcgagcgt gatcccgacg
     8401 acgaggggag tggcgatgac gacctgcgcg aggaccatcg cccacggggt gtagaggagg
     8461 tcgaggccgc cgaacggacc ggagcgggcg aggaggagga agaccccgag gccgacgacg
     8521 acgggcggga gacccattcc cgtgttcacg agcgtgacga gaaaggagcg tcccggaaag
     8581 cggtggagcc cgaggaagat cccggccggc acgccgacga gcatcgagac gacgatcgcg
     8641 accccggaga cgaagagcga gaggaggatg atccccgcga gctctcccgg cgccgggagg
     8701 agcgcgccga gcgacgcgag aggggccgtc atcccttctt cgcgtcgggg acgaagagcg
     8761 gctggccgaa ccgctccctc ccgaacgcgc cgatcgctcg ctgcgcctcc tccgagacga
     8821 gccattcggc gaagcgccgg gccgcggcgg cgcggacctg cgggtgcttc accgggctga
     8881 cgacgaggac gcggtaggag ttgcggagcg tcgcgtcccc ctcgacgagg accgcgaggt
     8941 cgagcgtcct ccggagcgag aggtaggtcc cgcggtccgc gagcgtgtac gccctcttct
     9001 ccgaggcgat tcgcgccgtc tcgcccattc cctggccggt cgcgacgtac cagctctgtc
     9061 ccgtcgggtc gaccccggcc tgcttccaga gctcgagctc gcgcgcgtgc gttccggagc
     9121 ggtcgtcgcg cgaggcgaag agcgcgccct tctccgcgag gcgcctcatc gcgtcgaggg
     9181 cgcggagccc gcgaacgcca gccgggtccg gcggaggccc gaggaggatg aagtcgttgt
     9241 acatgacgtc gagccggagc acgccgaagc cgccggccag gaactcgtcc tcggccgcct
     9301 tcgagtggac gaggaggacg tcggcgtcgc cgcggcgccc catcgcgagc gcctcgcccg
     9361 agccgacggc gatcgtcttc acgcggatcc ccgtcgcctt ctcgaagcgg ggaaggaggt
     9421 cgtcgaggag gccgctgtcc tgggtgctcg tcgtcgtggc gaggatgagg gagccctgag
     9481 cctccgcgcg gggcacggag gcgacgaggg cggcgagtac ggcagcgagc gagaggatct
     9541 tgcggtgcat ggagagtctc gcccgcgccc tgcgggacgg cgggcagggc atcctacttc
     9601 agccggaggg ggcgaaaggc gaaggcgccc cgccggcgag ccgggggagc gccttcgatc
     9661 gtcggagcgg gagccgtcag ggcgtcgccg ggacctcctc gaggacgccg gcccgctcca
     9721 tctccttcgc gacggaagcc gccaggcgct tcacgccctc gcggatccgc tcgatcggca
     9781 tgttcgagta gttcagccga agggtgttct cgttcccgcc gttcgggaag aacgcgccgc
     9841 cgggtacgaa ggcgacgttc tgccgcacgc actcgtcgag gagcgtccgg gcgttcaggt
     9901 tcgcggggag cgtcatccag aggaagaggc cgccctgggg acgcgtccag cggacgccgg
     9961 ggggcatctc ctgctcgagc gcggagagca tcgcgtcgcg ccgctcccgg tagacgggaa
    10021 tgatcttcgc gatgtcggcg tcgaggtcgt tctcctcgag gtagcggttc acctggagct
    10081 gggagagcgt cgccgtctgg aggtcggccc cctgcttgag gatgacgagc ttctcgatga
    10141 cggagaggtc ggcggcgacc caggcgattc ggaggcccgg gcagaagact ttcgagaacg
    10201 tgccgagggc gacgacgcgc cccttcgtgt cgaacgactt cagcgacggg acggactctc
    10261 cctcgagccg cagctcgccg taggggttgt cctcgaccac ccagacgtcg tggcgctcga
    10321 cgacctccat gaagcgccgg cgccgctcga gcgaccaggt tcgccccgag gggttctgga
    10381 acgtcgggat gacgtagatc gccttcgcgt tggggtgagc gcggagcttc gactcgaggg
    10441 cggccgggtc catcccgtcg tcgtcggtcg cgacctcgac ccagcgcggg cgcatcacgt
    10501 tccaggccga gagggcgccg aggtaggtcg ggctctcgca gaggacgacg tcgccctcgt
    10561 cgaggaagag cttgccggtg aggtcgagcc cctgctgcga gcccgaggtg acgaggacct
    10621 gctcgggcgt cacctgcgcc ccccagctcg cgttcatcct ctccgcgatc ttccggcgga
    10681 gcggcgggtg cccctccgtc gtcgagtact ggagggcgcg cgtcccctcc tcctcgaaga
    10741 cctcctgcgt cgcggcggcg aggcgcctca cgggaaagag ctcgggcgcc ggcagcccgc
    10801 cggcgaacga gatcacctcg ggccgctgcg tcaccttcag gatctcgcgg atctcggagg
    10861 ccttcagggc ttccatccgt cgtgcgaatc gggtcgtcat ggggtccccc ttgtcgccgc
    10921 cggcctcgcg gcccccggcg tttgaatgaa gctcagacga tgggcggcgt gcccaccacg
    10981 gcgcggctcg gccggcgcgg ctcggcctgc gcggcgcgga gcgcgctccc gaggcgccgc
    11041 gccccctcgc gcaggtcgtc ctcgggggcg aacgtgaagt tgaggcggat cgagctctcg
    11101 gacggctcct ccacgaacga ggccgccccc gggaggtagg cgacccgctc ggccgcggcg
    11161 tgggcgacga ggctcgccgg cgagacgtcg tccggcagcc ggcaccagag gtagaagcct
    11221 ccctccgggc ggctccagga gagcccgggg accgcctcgg ccgcgagcgc ctcgaggagg
    11281 acgtcgcgcc tccgcctgta tgcggcgcgg acccgggcga ggtgggccga caggagcccg
    11341 gaagagagga acccctcgac gatccactgc cccagcgtgc cggagtggag gtcgaccgtc
    11401 tgcttcacga gcgccagctg gcggacgagc ggccgcggag cggcgatcca gccgaggcgg
    11461 aggcccggga agaggacctt cgagaaggag ctgaggtgga tcacgtgccc gtaagggtcg
    11521 agcgccttca gcgacgggag cggcgaaccc tcgtagcgga ggtcggagta ggtgtcgtcc
    11581 tcgaggaccg gcacctggtg gcgccacgag agctccagga ggtggcgccg tcgcgcgggc
    11641 gagaggacgg cccccgacgg gttctggaac gtcgggagcg tgtagacgag gcggggcttc
    11701 tggcgggcca ggagcgcttc gaggatgtcc gtccgcatcc cctcggcgtc cacgggcacg
    11761 ccgaggaggc ggacgcgcgc ggagcggaac gcctcgagcg ccccgaagta ggacggctcc
    11821 tcgacgacga cgaggtcgcc gggggagagg agtgtgcgcg cgacgaggtc gagcccctgc
    11881 tgagagcccg acgtgacgag gacctcctcg gggctcgtcc ggatgccgcg cgggaccatg
    11941 tggcggcaga gcgcctcgcg aaacgccgtc actccttccg tcgggctgtg aagcagcgcc
    12001 tcgggcccct tgcggacgag gatctcctcc tgtacgcgcc ggagctcggc gagcgggagg
    12061 agctcggccg cgggaagccc ggcctggagg ctgatgacgt cgtgccgctc ggtcagcgcg
    12121 aggacgtcgc ggacgagggg gtcgggctcg cgcacgcccc cgtcgcgggc gagctgccgc
    12181 catgggagcg gtccggccgg ctccgcttcg ctcctcagca cctgctgcga cgcgacctcg
    12241 gtgccgcgcc cgacgtgcgc ctcgacgagg ccgtcggtct tcagctcccg gtaggcggag
    12301 aggatcgtcg tccggttgac gccgagcgcc ttcgcgagcg tccgctcggg cgggagccgg
    12361 aagccgtcgg ggaggcgccc ggagaggatc aggtcgcgaa tgccgccccg gatctggagg
    12421 tagacgggga cggcgctctg gcgatcgatc cggatgtcca cgctccgcct cccttcaagt
    12481 ctttcttacg gaccaatctt cagattggcc tgatccgaat gacagtatga gtcatccatt
    12541 ttcgttttca agagccattc ggagggcgat tgggccagcc aatctggacc cgaggggcct
    12601 cgagcgagaa ggggtcgagc gagaaggggt ctggtctaca ctctacactc tgcgatgagc
    12661 agagtgtaga gtgtagacca gacccctcat gtcggacccc tcatgtcggg cccctcccgg
    12721 cggctccagc gggaggggcc cggggtggtc gccgagctac ttcgccggcg tgcccttcag
    12781 gtgggtttcg aggaagcgga gggcgtggcc gagcatgagg acctggttgt cgcgcttgcc
    12841 ggcgccgtgg ccttcgtcgg cgtagacgat cagctccgtc ggcacaccgg tcttctgcgc
    12901 cgcctcgtac atctggatcg cctcgcccac cgggacgcgc gggtcggtcg ccccctgctg
    12961 gatctggagc ggcgccttca gcttcgcgag gtgcgtcgtc gccgagagct cgaggagcgc
    13021 ctcccggtcc ttctcggggt cgccgtactc ggtcgttcgc aggatgcggc ggtagggggc
    13081 ggtgttctcg aggaacgtga ggaggttcga gatgccgacg atggaggctc ccgcgtcgta
    13141 ggccccggcg aacttcgtca tcgcgacgag cgtggcgtag ccgccgtagc tgccgcccgt
    13201 cacgccgact ctcggctcct tcccgttcgc cgcgaacgcc ttccgggccc aggtcgcggc
    13261 gtcctcgatg tcggtccaga cgtcgaggcg cttccggccg ttgtcggcgt cgagccaggt
    13321 ccgcccgtag ccgtcgctgc cgcggacgtt cggctcgacg aagacgaatc cggcgtcgac
    13381 gaaggcctgc gcccaggtcg agaagccggg ctgggcctgc ccctccggtc cgccgtggaa
    13441 ctcgacgacg accggacaag gggcggggtc gcagctcttc ggccggcgga cgaacgccgg
    13501 gatcttcgtc ccgtcgcggg ccgggtagtg ctcgagcgtc gcggcggcga aggtcgtcgt
    13561 gtcgatctcc ggggcgctcg ggacgaccca gcgggtgagg gcgccgctct gccagtcgta
    13621 gacgtacgag gtgcgcggcg cggtggcggt ctcgacgccg aacgtcgtga agcgcccgtc
    13681 gcgggtcgag gcgccggcgt agacgtggtc cgccccctcg gggagcttcg ggaggcggac
    13741 cggctggaac gtcttcgcgt cgaggacgaa gagcttcgtg tagccggctt cgttgaccgt
    13801 gtagaggacg cgcgtcttcg cctcgtcgag gctgaagccg gagacgtccc acgagaggtc
    13861 gccggtcagg ggcgtcagcg ctcccttctc ccagcggcag agccggcgga actcaccgag
    13921 cttcggcgtc aggacgagga gctgccccgg cgcggcgccg tagacggccg tgtactcctc
    13981 gggcctgtcc tggccgagga ggggcgtcag cttccgggtc gagggcgacc actcccagta
    14041 ctccgaggtg agcgatccgg tccacttcgc gaggaggagg gtcccgtcgg gacggtggtc
    14101 ggagacgctc cagaggcccg gctcggtgac gagggcctcc ctctgccgcg tcgcgacgtt
    14161 ccagcggtag acggcgtagg agtcgggctt gacgtcgttc gacgcgaagt agacccacgc
    14221 gccgtccggg ctgacgaact ggtggaaggt ctggatcccc gggagatgct ggatcgaggt
    14281 gagcggtccg ccggcggccg gcatcaggta gaggccgggg ttctcctctc ccttccggtc
    14341 gcgctgaagg accaaggtct tcccgtccgg aaagacgtcg acgaccgtcg tccgatcctc
    14401 gccgcccgtg agctggacgg ggaaggtgtt cgggccgtcg agccgccaga cctgggaggt
    14461 cccggtgacg gaccatccga agaagagctt cgagccgtcg ggcgagacga tccccatccc
    14521 gggcgcgcgg acgtccatca tcgactggat ccggcgggcg aggtcgggcg ggagcggctt
    14581 cggcgcgtac cgcgcgatca cctcggcggg gacgctgtcg gcgccgtggc ccttgtaggc
    14641 ggtctgcggc tcgccgaggg cggggagggc gaggagcaac gcggcgagag gggagaaacg
    14701 gcgagaggcg ggcatcggtc ctccttcagg ttcgtccggt gtgcaccgga atgttacggg
    14761 gaggagggcc gccgggttcc tggagggagc cgcgaggacg gcgccgggcc ggaaagaagg
    14821 aaggccccgg ggcgcgagcc ccggggcctt ccgaaatcca tgggaggagg ggcctacttc
    14881 gcgtccgcgc ccttcccctt caggtgcctg tcgaaccact ggatggtgtg ccccgtctgc
    14941 aggacccggt tctcgcgctt ctgcgcgccg tgtccctcgt cggggaagat gatgaggccg
    15001 gtcgggacgc cccgcttcgt cgtcgcctcg tacatctgga ttgcttcgcc caccgggacg
    15061 cgcgggtcgg tggcgccctg gatgaggagg agcgggtcct tcagcctgtc gatgtacgtg
    15121 acggccgaga gcttctcgag ggcctcccgg tccttctcga ggtcgccgta ctcggaggcc
    15181 cggaggatgc ggcgatacgg agcggtgttc tgcaggaacg agacgaggct cgagatgccg
    15241 acgttcgaga cgccggcgtc gtaggccccg gcgtacttcg tcatcgcgat gagagtggcg
    15301 tagccgccgt agctgccgcc catgacgccc agcttcggag ccttgccgtc gacggcgaag
    15361 gccttccggg cccagagcgc cgcgtcctcg atgtcgctca ggacgtccag cctcttcggg
    15421 ccgttgtcgg cgtcgagcca ggtcttcccg tagccctcgc tcccgcggac gttcggctcg
    15481 acgaggacgt acccctcgtc gacccagagc tgggccatcg gggagaagcc gggagtcgcc
    15541 tgcccctcgg ggccgccgtg gaacaggacg acgaccgggc acggagcggg gtcgcaggac
    15601 ttcggccggc ggacgaaggc cgggatcttc gtcccgtcgc gagccgggta gtgctcgagc
    15661 gtcgcgaccg cgaacgactt cgtgtcgacc tcgggcgcgc tcgggacgac ccactgcacg
    15721 agcttcttct cccgccagtc gtagacccag ctctgggaag gcgcggtcgg ggtcccgacg
    15781 gcgaagctgg tgaagcgtcc gtccggcgtg gacgagccga aggtggcgtg gtcggccgcc
    15841 gggagcttcg gcatctcgag cggcttccag gtcttcgcgt cgaggccgaa gaccttcgag
    15901 tagcccccct cgttgaccgt gtagaggacc cgcgtcttct tctcgtccat cccggcgcgg
    15961 tccacgtccc aggggagctc gggggtgatg ggggtccact tgcccgcctc gaagaggtag
    16021 ccgcgctgga actcgccgaa ctcgggcgtc acgacgacga gctggccctc cttcgggccg
    16081 tactgggcgg tgtaccggcc gggcttctcg gtcccgagca tcggggcctt cgccttcttt
    16141 gccgggtccc actcccagta ctcgaaggtc atggagccgg tcgccttcac gaggaggatc
    16201 cgtccgtcgg gcttgtggtc ggcgacgaac cagaggcccg gctcgtcgaa gaccgtctcc
    16261 ttcttcccgg tggcgagctc gtaccggtag atggcgtacg agttcggctt cacgtcgttc
    16321 gaccggaagt agagccactt cccgtcctcg ctgacgaacg cgaaggaggt ctggaccttc
    16381 gggaggtgct ggacgacctt catcgggccg ccgtccgccg gcatcaggta gaggccgggg
    16441 ttctcctcgc ccttgcggtc gcgcgagagg atcagagtct tcccgtcggg ggtgacgtcg
    16501 gcgagcgacg tgcggtcctc gcctcccgtc atctggatcg ggaaggtgtt cgggccgtcg
    16561 aggcgccaga cctgggcggt cccggtcacg gcccagccga agtagaggcg tgcgccgtcg
    16621 ggcgagagcc ggccgccgct cggagcgcgg acgtccatca ggttctggat cttccgggag
    16681 agctccgacg ggagaggggg ggcgacgtac ttcgcgagga cctccggagg gacgctgtcg
    16741 gcgccatggc cctggtaggc cgtctgctgg gcggcggccg gagctgcgag aagggcgagc
    16801 gcggctgcga gggccgcgat ccgtcgggct cgattcatcg atcctcctgt cgtgtggtac
    16861 ggccgcgccg gaggcggggc aaacctctca ccgggtccgt cacctcgaag atacgtgcgc
    16921 gccgccggaa ggttgacggc gacgcgggga ctcaggtctt cggcgtccac gtctccggca
    16981 ccttcacgag gatcgcctcg ccgcgcgcgc agaggacccc cttcgagaag agcgacgtgg
    17041 cgaggacgac cttgcgctcc ttcacctcga cgatcgtgga gcgaagctcg accggggcgt
    17101 cgatcggggt cggcttcagg tattcgacct tcagcgtccc ggtcaggaag cgcgggtagg
    17161 ggccgaccgc cgggtcgcct ccggccgcac ggtgcgcggc cgcggcggcg ctcccggtcg
    17221 aatggcagtc gatgacggag gcgatgaagc cgccgtagac gaagcccggg accgaggtgt
    17281 ggtgcggccg cgggacgatc gtcgcgacgg tctcgtcgcc gtcccagaag ctgcggatct
    17341 tgaggccgtg ctcgttgagc cggccgcagc cgtagcagag cgcccagtgg tcggggtaga
    17401 ggtcctggaa ggcgggggtg tcggtcacga cgggctcctg agggcgcggg tggcgcgcgc
    17461 gagggcggcc ccgagctcca tgagggactt cctgcgggcc gggtcggcga cgttgccgtc
    17521 ggcgtcgaac gcctccgcgg ctttcggaag ggcgacctgg tccgggagga ggacgacgcc
    17581 gaggttcccg aggacgacgc ggaccgacgc gagcccgcgg atgccgccga gcgcgcccgt
    17641 cgaggcggag cagagggcgg cgaccttgcc cttgaagcac gcgagcggct tctcccccgg
    17701 ggcggggcgc gagacccagt cgaggacgtt cttcagcgtc ccgctgatcg ccgcgttgta
    17761 ctcggggctc gcgatgagca gcccctcggc cgcgcggaag cgctccttga gcgcgagggc
    17821 ggccgccgag agccccgagg ccgactccag gtcggcgtcg tagacgggga gcggaaagtc
    17881 ccgcaggtcg atccccgtca cgtcggctcc cgcggcgcgg gcgccgtcgg ccgccaccgc
    17941 gaggacgcgc ctgttgtacg agccggcgcg caggcttccg gcgagggcga ggatccgcgg
    18001 ggcgtcgctc atcagtctct cccgacggcg cggatgatac ggggctcctt cgctcagctg
    18061 cgacttcggg tgcgctcgcg cgagcggggc gaagggcgcg gagagggacg ggagccggga
    18121 cgggagggag gacgggacgg gggggccttc tcgcgggccg gtccgctctc gctcgggcgc
    18181 gagaagccgc ggcgacggag gaggtcccac gtccgctcct cgagaagcgc ctgcgggacc
    18241 ggctcgggca ggaggtccgc agcgcccgac tggagggcga acgcgtgctt ggcgcggtcg
    18301 gggcccgggg ccagcaggag cacgggaatg tgggcggcgc gcgggatcga cttcaggatt
    18361 cgcgcgacgt cgaacccgtc catgcccggg agtcgcgtgc tcatcaggac gagatccacc
    18421 tggcgggaga gggcggtctc gagggcctcc tcggcgctgc ggacctcgag cgtccggcag
    18481 ccggtcatgc agagggtcgt cgcgagcttc aggaggccgc gcacgtcctc ctcgacgacg
    18541 aggacgaccg gcggccggcg ccgtgtccgc gtcgagacga gcgtcttcgg cgcgtcgccg
    18601 cgcgaggcgg gccgcgcctc gcgcggcagg atgatcgagt cgatggcgac ctccacctcg
    18661 cggaggaacg cctccgcctc cggcaggagg cgctcctcgg ggagcggcga ggccgggccg
    18721 gtgtcgcaga gagaaagggc gtcgtccagc tcctgggccg atcggaagcg gtcctcccgc
    18781 ttcttcgcca ggacccgcat caggacggcg aagaggtccg cgggcaggcc gggagccagc
    18841 tcccggaggt ccggcagcgg ctcgtggacg tgcttcatcg ccgtcgcgac cggggagtcg
    18901 ccggcgaacg ggagctggcc ggagagcatc tcccaggcga tgacgccgat ggagtagagg
    18961 tcggcccgct cgtcgagggg gtcgccgagc gcctgctcgg gggacatgta ctcgggagtc
    19021 ccgaggagga cggaggcgag cgtgaggccc gggtcgaacg agccgcgggc gaggccgaag
    19081 tcgaggatga cggcggcgcc gtgccggtcg acgaggacgt tctgcgactt caggtcgcgg
    19141 tggaggatgc cgagccgatg gatcgcggcg acgccgcgcg cgatctgccg cacgatcggg
    19201 accgcctcgg ccgcggagaa ccgctgccgg ctcccgatca gcgtccagag gtcctcgccc
    19261 tcgatcagct ccatcgtgat gaacgggtag tcgcccgcga tcccgtagtc gaacatccgg
    19321 gcgacgttcg ggtgcttgat cttccggttg agcgcgacct cgcgcttgaa gcgggcgagg
    19381 agcgcgttct cgtcgcgcgc gaggttcgga gagaggacct tgatggcgac gctctccccg
    19441 agctcgcggt cgagcgcccg gaagacgact ccgatccctc cgcgcccgag cgtcccctcc
    19501 cactcgtagc gcgggaggag ctcgaagaac cggcgccgaa acgactcgag cttcaaggcc
    19561 tcccccgcgg cggcttcagc gcggggccgc ggacgtcgcg cggccgtcga ggaggaagac
    19621 ctctccccgc gtctccgcct gctcgtagag gatccggtcg cccttcggcg accaggaggg
    19681 gtagcggatg tagacgtcga gccggaggtt ctccgtgagg cgtcgctcct cgcgcgtgtt
    19741 ccggtcgatc caccagaggt tccaggtgtc gtcgcggtag cccgcgaacg cgatccgccg
    19801 gccgtccggc gagtagctga atgcccagga gagcccccgc ttctgggtca ggagctcggg
    19861 agtgccgccc gccgacggga tgacgccgat gaagatgtcg tctccccgcc ggacctcgag
    19921 ggcgagctcg cggccgtccg gggaccagca cgggtagccg acgaactcgc ggtcgaacgt
    19981 cagctgctgc gaggtccgcg tcgcgaggtc gacgacccag gcgttgatcg tgatgctgtc
    20041 gcgcttcgtg tggtaggcga tcctcttccc gtcgggcgag aggcgcagag cgatggcgtc
    20101 gtggaggtcg gccgggatgc gccggaacgt gcgcgtcgcg atctcgagct cccacggagc
    20161 gaacgttcct ccctcccggt tcgagaagaa gaggagccgg cggccgtccg gaaagaagtc
    20221 gctcgcgtag tcgatcgacg ggttctcgac cacgggtcgc tggctcgccc cgtcgggctc
    20281 catcagccag acgtcgttcg tcccgcccgg agggacccac ccgaacgcga cggtgctccc
    20341 gtccggggag aagacgggcc acgtcgcgcg gccgtcgccg ccggtgagcg gcgagggcga
    20401 tccggcggag agcgaggtcg cggggtcgag cggaaggctc cagatcgccg cggtcgtcgt
    20461 cagctcgctc caggccagcc gggagccgtc cgcggagaag ctcgggctgt gcggcacggc
    20521 cgagcccggt ggcgtcatcg cctcggccgg gccgacggcg gtgcgacccc tctcgtcgag
    20581 ggagatccgg tgaagggcgt agagcgaggc ctttccctgg cccccgaacc agagggcctt
    20641 cgctccgcgc gggacgcaga ctccgtagat ccgcgttccg gcgtggagcc gcacgaggtc
    20701 gctgccgtcg ggcttcaccg accagatctc cgcctgcgtg aggcgcggct cggacgtgac
    20761 gaagtagatg cggtcgccgt cgggcgacca ggcgggcgcg ccgtggccgc cgatcgggct
    20821 ccccgggttc gtcacggcga gcgccgcccc cccggccgcg tcgacgaccc agatcgtcga
    20881 cggagcgagc cccggcgcgg ccgtcgccgc gacgtcggtg agcggcgtcg actggaacgc
    20941 gacgcgccgg ccgtcggggg agaaggaggg ccgggagccg aagctggcga ggaggcgcgg
    21001 tgagcctccg gaagccggga cgatccagat ccccccacgg tccttcgaat ggaacgcgat
    21061 gagccgtccg tcgggggacc aggcgggctg gacgttgttc cggccgtcgc gggtgagggg
    21121 gcggcccggc ccgccggaga gcgagcggac gtggatctcg agcttgccgt tcgtcatcac
    21181 cgacgaggcg agctcgtcgc ccgagggcga gagcgcgggg aagagctcga gcgcgctcgc
    21241 cccgccgagc ggtctcgggg tgcccgaggg gagcggggcc ggcatcgtgt cgccgccacc
    21301 gcagccggcg gcagcgacga cgacggcggc gaggacggct cgaaggcgcg acgtcgcccc
    21361 gcgcggtaga gcggctcggt gtctcatcgg gattcccgcg accggcatct tagccccggc
    21421 agcccggacg gggcgacggg gtgacggagg cccgcgcgcg acctccaacc ccgaggccgg
    21481 gccggcgcta cgatggacgg tcggcccgcg gaccgggccg acgtaccgat ggtccgctcc
    21541 ttgcggcagg agagacgacg gggcggaacc gggaacgatc ggaaggcacc gacagaggag
    21601 accgatgacc gagacggaac ggccgggagg cggatggcgg atcttcacga tgggcctcgg
    21661 cagccgg
//
LOCUS       DAOTEZ010000014        21456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_1617_length_21456_cov_6.661184, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000014 DAOTEZ010000000
VERSION     DAOTEZ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 21456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21456
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1617_length_21456_cov_6.661184"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2241)
                     /gene="ppdK"
                     /locus_tag="P5164_01400"
     CDS             complement(<1..2241)
                     /gene="ppdK"
                     /locus_tag="P5164_01400"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008387626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRY42575.1"
                     /translation="MTSKSAPTARGAKYVYSFGGGSAEGDGSQKNLLGGKGANLAEMA
                     RIGLPVPPGFTITTEVCTHFYANGRSYPKGLEAEVARHLAGVEKKLGKKFGDPKNPLL
                     VSVRSGARASMPGMMDTILNLGLNDVTVEGLATASGNPRFAWDCYRRFVAMYGDVVLD
                     LKPVGKHERDPFELILEAKKEKFGVHLDTELTVEALKELVGEFKKAIKEKTGHDFPED
                     PMKQLWGAVGAVFSSWMNERAIVYRQLNSIPESWGTAVNVQSMVFGNLGEDCGTGVAF
                     TRDPATGENLFYGEYLINAQGEDVVAGIRTPQKIATLQQAMPKVYDQLMGIRAKLEKH
                     YRDMQDVEFTVERGRLFMLQTRTGKRTGFAEVRIAVEMEREKLISPEEAILRVNPDSL
                     NQLLRPVFDPNAKKAAVAEGQLLAKGLNAGPGAATGRVVFSAQDAFEWAKKGEKVLLV
                     REETSPEDIKGMNAAEGILTARGGMTSHAALVARQMGKVCIVGCESLRIDYAQRCMTV
                     RGDVKNRVVMEGDWLSIDGSTGEVLEGRIATKPSEVLQVLLEKTLKPEESPVYTLYAT
                     MMGWADKVRRLRVRANADQPDQATNAVLFGAEGIGLCRTEHMFFGGDRITAVREMILG
                     DTVEAREKALAKLLPMQRGDFEGVFRAMGPRPVTIRTIDPPLHEFLPHKADEQKDVAR
                     QLGISPAAVAQKVNELHEMNPMLGHRGCRLGIVYPEITRMQARAILEAACNVKKEGVE
                     VKPEIMI"
     gene            2537..3835
                     /locus_tag="P5164_01405"
     CDS             2537..3835
                     /locus_tag="P5164_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRY42576.1"
                     /translation="MPLLLLLPLLLSTSVLADGTPLTLDEAIRLAVTRNERALASGER
                     LEAAEARVSKARAFFFPDVQAAGNYTRRPFERTSTVGGQEVVLSKRDAFSSSITARQV
                     LFDARALPLWRQARSDRDAASLEAAEERRLVAFEAAAAFLRTLGADQVAGAAERRVEL
                     ARKNLEDARARFEAEITSSNDVTRAELELATAEREATRARNDEETSRLSLANLLVSDV
                     PEALEVPQSLLATADRERPAEEELVTRARERRLDVAAGLKRAEALHFWAKEPMNRFFP
                     SLVARADGRWTNEGGLNARNTTWTYGLDLSWSLFDSATRFADRRERNANARAADLNAR
                     ARERAVELEVKAALASLDNARAAVRQAAVALEAARKNASETTTLYREGLANALAVADA
                     NQSLFEADVALTNERYGLGAAVLDTWAALGLDPAGKEPLP"
     gene            3832..4980
                     /locus_tag="P5164_01410"
     CDS             3832..4980
                     /locus_tag="P5164_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY42577.1"
                     /translation="MKRPTLAPVLLLVGTAALAFSCSGGGEKKGASGGKGGPPAFPVE
                     TSPVRSRPVEYTVTAVGSVEAFEKVQVTARVAGVVEQVKFVEGESVETGTALVEIEPR
                     RYQLAVEAARANVQKAEAAKADAEAALARRRAVVEKNPGLIPGEEIDTFATRVQTAAA
                     DVAQAKSALDQAELNLRDAFVRAPVAGRIETRTVTTGQYVAPGGVLATLVRRDPLLLR
                     FHVPSEEARSLRPGLVARFRVPGDAKEFTARITHVAGAAEAASRMVAVTAQVDDPRRA
                     QLRPGAFADVSVPVGGIADSPVIPQTAIRPSEKGFLAFVVEDGKARERVLTLGLRTTE
                     GLVEVRGGLKAGEELVIRGAEALRDGLPVKVSAPGAEPTAPSRPEARG"
     gene            4977..8066
                     /locus_tag="P5164_01415"
     CDS             4977..8066
                     /locus_tag="P5164_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRY42578.1"
                     /translation="MNITEVCIRRPVLAWMLMAATIVFGGVALSRIGISQFPDVDSPT
                     ISVSVTWEGAAPEVMEHDVVEPLEEAVMQVEGIKSLTSSSRQGSANVTIELDISRDID
                     LALQDVQTKVSQAQRSLPRDIDPPVISKSNPEDNPILWIGLSGPFPRQVIADYARYRL
                     KERFQTVPGVGEVNMGGYLDRNVRIWVDADKLNSKGMTVTDVIAALQREHVELPAGRL
                     ETEGREISVRVMGEALDLATLRKISLRDLNGAPVYLEDVALVEDGFEDERRIARVNGA
                     PAQGLGIRKQRGSNAVAVAQGVKQAMAEIQKTLPPGMEVGINFDSTTFIEDSVHEIQF
                     EILLSVVLTGLVCWMFLGSLSSTLNVVLAIPMSLLGTIAFIYFAGFTLNTFTLLALGL
                     AVGIVVDDAIMVMENIFRHFEEGKDRVTAAREGTGEITFAALSATIAIIAIFIPVVFM
                     KGVIGKFFLQFGITLCVAVALSYVEAITLAPARCAQILKAGEVNRSGLGGLVDRAFDA
                     LARVYARVLAKGLKHPVAVLVSATLLLGVAFLVFRALPSEFVPSQDQGRLMLRVQTAV
                     GSDIRETDMAFRKIENYVTSRPEVTRCFGVVGGFGGGVSGGVVFVSMVDKSDRKLSQA
                     EFAAEIRKELNSYPGVRVVVQDLSQSGFTAQRGFPVEFSVRGPDWDVLVEKARGISER
                     LNASGQVVDLDMDYRIGMPELRIVPNRARAADLGISVENVATALNATLGGLRIGKYST
                     EGRRIDVRLRLLAGQRTRPEDLSRLRLRSSTGDLIPLSSLVTTEEVPALQAITRRDRE
                     RAITIYANVAPGMSQADVLKYVEGLGSDMPTGYRLVLGGASVAFRESMGDLLFALVLG
                     IGVAYMVLASQFNSFLHPVTVLSILPLSVAGAAFALLATGRSLNIFSMIGLLLLMGIV
                     KKNSIILVDYTNQLRERGLDTLSALLKAGPVRLRPILMTSIATMMAAVPPAVGIGSGS
                     EIRAPMGVAVIGGLIVATVLSLLVVPSFYLVTDRIRDRVQGRRREPATPSEPSASAP"
     gene            8155..10071
                     /locus_tag="P5164_01420"
     CDS             8155..10071
                     /locus_tag="P5164_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY42579.1"
                     /translation="MIAVSDLGKAFGAQTLFEGVSIQFNPGNRYGLVGANGSGKSTLL
                     RILSGEESPSSGVVSIPRKLTLGVLSQDHYRYEEMPILDVAMMGNREVWEAMAEKERL
                     LHADSEFHGDRYAELEDLILRNQGYSLEARAGEILEGLGIPTEVHRSPLSTLSGGFKL
                     RVLLGQVLAADPGALLLDEPTNHLDIVSIRWLEKFLVSYKGLAIVISHDLRFLDNVCT
                     HIADVDYETVTLYPGNYTAFEAAKEAERERKEAEIQKREKQIAGHQAFVDRFRAKATK
                     ARQAQSKIKLIEKIVIDKLPESSRRHPRFRFRQRRPSGRTALEIESVGKAYGDNVVLK
                     DVSLRVERGDRLAIIGPNGIGKSTLLKIAVGELAPDAGKVTWGYEASPGYFSQDHAEL
                     AGAGRQSIEAWLWEAAPGEPIGFVRGHLGHVLFTGDDAEKPVRALSGGEAARLVFARL
                     SVLGPNVLVLDEPTNHLDIEAIEALVTALLDYDGTLVFVSHDRWFVSRLANRILEISP
                     KGLNDFRGTYDEYLERCGDDHLDAEAVLQSLRKARKSQDARKKEPEPKRQNRVKALER
                     RRDELTSLIEAAESRVHAISERFLDPALFGKGAQAETRKLEEEQKQLRARIEGLMSEW
                     EQAERELEAERPGA"
     gene            complement(10075..11259)
                     /gene="argJ"
                     /locus_tag="P5164_01425"
     CDS             complement(10075..11259)
                     /gene="argJ"
                     /locus_tag="P5164_01425"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015761110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HRY42580.1"
                     /translation="MNLPKGFLGSAVAAGLRKKPGLDVALLVAPGGASAAAVFTRNRF
                     QAAPVLLSRASLRKSRGRVKAVVVNAGCANAVTGAEGLAAARRVQERAADLVGCPREE
                     VFVASTGVIGVVLPDAKVTTFLPDAFARLSSGGIEAVSRAILTTDAAPKVARATFRHG
                     GKTCRVLGVAKGAGMIHPNMATMLAFVTTDAPASPALLQAALKEAVDGSFNAISVDGD
                     TSTNDTVLLLASGASGGRELRSIDGAGAFRAALAKVCDELAWRIVRDGEGARRVMEIT
                     IEGAATEREAKLAAHAVATSQLVKTALYGGDANWGRILAAIGRSGARVSTRRVSLRIG
                     RLSVVEDGAPTGYREAAAAKAFARERVPLVADLGVGKARARLLAADLGHEYVTENADY
                     RS"
     gene            complement(11256..12653)
                     /gene="argH"
                     /locus_tag="P5164_01430"
     CDS             complement(11256..12653)
                     /gene="argH"
                     /locus_tag="P5164_01430"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRY42581.1"
                     /translation="MSPRHSLWGGRFSASPAEVLRRFNDSWSFDRALIAEDVAGSIAW
                     AEELGRCGVLSATEARKIVKGLRDVLAAWEAGEGRDSDAEDVHSYVEGELGKRIGPLA
                     GKLHTGRSRNDQVATDLRLWLRSAFDALHAALLDLAAALATRASEEAATPMPGYTHSK
                     RAEPVTFGHWCLAYVEMLLRDADRVRAARKRANECPLGSGALSGTPLAIDRERLARSL
                     GFERATASSLDGVSDRDAAVEYLFVAALHLSHLSRMAEDLIFFTSDEAGFAELPDALA
                     TGSSRMPQKKNPDLLELARGHAGRAVGELTGLLAVLKGLPLAYDKDLQLDKEPLFRVR
                     GVLDAALPAMTALVAGLKLRRERMRNAATDDRLLATELADALARRGVPFRQAHEIAGR
                     RFAAAEASGQGLLERTPSDGVTEADLASVDLGRALARRGAVGGTAPKRVAQAARRAAK
                     RIAAAQKAAAGRSVR"
     gene            complement(12650..13906)
                     /locus_tag="P5164_01435"
     CDS             complement(12650..13906)
                     /locus_tag="P5164_01435"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRY42582.1"
                     /translation="MSATKIKKAALAYSGGLDTSIIIPWLKENYGCEVVAVAVDVGQA
                     EETSGLKEKALATGACDFHLIDAREEFAAEYLWPVLRAGAVYEHEYLLGTSTARPIIA
                     KKQVEIALATGCDALSHGCTGKGNDQVRFELAYQALAPELTIVAPWREWTINSREDAI
                     DYAAARNIPVPVTKKEPYSRDRNLWHISHEGGPLEDPAFEPEESMFKLTVDPRKAPDE
                     PERVTIGFEGGYPVEVNGKLLPAAELLVTLNAIAARHGVGRIDMVENRLVGIKSRGVY
                     ETPGGTLLVQALRALETLTLDRDAMHEKERLALRYAELVYYGQWFSPIREALEAFMEK
                     VTETVTGHVTLKLFKGSAIVVGRRSEHSLYSANLASFNMTGYTAHDATGFIRLFGLPT
                     RGRKRLVPPTDAAVKAGEKLRLEPWG"
     gene            complement(13903..>14524)
                     /locus_tag="P5164_01440"
     CDS             complement(13903..>14524)
                     /locus_tag="P5164_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY42583.1"
                     /translation="QGEGGIVPLAPEYLRAARELCDAAGAVLVFDEIQCGLGRTGKLF
                     AFQRTGVVPDVVTLAKPLGGGLPLGAVLLNERVASLVKPGHHGTTFGGNPVACRLGLA
                     VLEELVEGGLVAKVEEIGEWFGKKLAQLSKRCPAVLDVRGDGLIRGIELDREAGPVQK
                     KLLAKGFVVGTARKNVIRLLPPYVVPKAALSAFLTTLEQILKEGAA"
     assembly_gap    14525..14534
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14535..15089)
                     /locus_tag="P5164_01445"
     CDS             complement(<14535..15089)
                     /locus_tag="P5164_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY42584.1"
                     /translation="MSRPDESPAAVEARAAAYLLGTYSWTPFHPRDGRGAKLVDAEGK
                     VYWDLLAGIAVNALGYRHRRLVKTLKAEATDLLHVSNLFYQPAQGELAERLVTLSGLS
                     RAFFCNSGTEANEAAIKFARLANPGRSKLVALDNSFHGRTVGAVSLTGNPPYREPFEP
                     LLPGVTFLPANDVNALRAAVDDTAS"
     gene            complement(15076..15852)
                     /gene="argB"
                     /locus_tag="P5164_01450"
     CDS             complement(15076..15852)
                     /gene="argB"
                     /locus_tag="P5164_01450"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRY42585.1"
                     /translation="MIDVVKLGGSLLENKALRHSALEAVATAWSSGKRSIVVHGGGKR
                     IDAMLKALDIPKKVQGGLRVTDAETLEVVVSILSGLVNKTLVAELRSMGILASGISGA
                     DGDTLWAEFHEAVEGVDLGFVGRVVRCDPKLLSAVLSASFLPLVASVALGREGTLLNV
                     NADSAASALAGALGAKRLVFLTDVEGVLDADGKVIEQLDARAGRALLASPAVTGGMKP
                     KLRAALDALDAGVSEVLVAGPSLHRAALLEGKGGTCLVPA"
     gene            complement(15849..16868)
                     /gene="argC"
                     /locus_tag="P5164_01455"
     CDS             complement(15849..16868)
                     /gene="argC"
                     /locus_tag="P5164_01455"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRY42586.1"
                     /translation="MIRVSVVGAAGYSGAEAVRLLSRHPGVELAGLFGSPGGKGAPFQ
                     DLHSDLGGASGPDVVPFSEEVLLSCRPDAAVLATPNEVSAEVAPRLLAAGVRVIDVSG
                     AFRLKDASLYPAWYGFTHPAPSLLTEAVYGLTEWCGEELEGAKLVANPGCYPTSVLVA
                     LKPLASLLDPSQPVLVTSASGVSGAGKKADLAYSFCEVAGSYRAYGVGTHRHEPEMRQ
                     ELGLASEAAFAFVPHLLPLVRGILSTIHVSFDAAVSDAAIAERYAAAYAGKPFVKVLS
                     AGRLPELKEVVGTPRAHVGWKLLPGGRRAVVVSAIDNLLKGAASQAVQNLNRLFGMPE
                     TEGLL"
     gene            complement(16906..17400)
                     /locus_tag="P5164_01460"
     CDS             complement(16906..17400)
                     /locus_tag="P5164_01460"
                     /inference="COORDINATES: protein motif:HMM:NF014864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42587.1"
                     /translation="MPNNHSNASVLRRRDAILRLVREEVVRSQEELQRLLAERGFEAT
                     QPTLSRDVRELGLAKTPGGYAPQGTHDRAADVAQRASRLQRTLSEAVLSVRVAGSLVV
                     LRTPPAGAHPVARAVDEAELAEAIGTIAGDDTVFVATPGAVSARALAARLAGPPSADR
                     RRHA"
     gene            17648..19849
                     /locus_tag="P5164_01465"
     CDS             17648..19849
                     /locus_tag="P5164_01465"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRY42588.1"
                     /translation="MTDSTRSELFDRLRAGIVLLVVLAASFALAATASAKPPTDDDCL
                     ACHSEPAGKGKKAPQKALSVSVHGEAGLACVDCHADLAKLKELPHGPKVAPADCTTCH
                     DSVAESHAFHPMMKDDPTAIACADCHGGHDVARVADAGSPLAGAAVRKVCAACHDTEV
                     GLYADSAHGRAAMAGVQEAPACLACHKTALVSVPDGAPPASAKLAQEKLCLSCHLDNP
                     DVRSRVSPSAGFIAKYEESVHGRALLAGNEKAANCVDCHGAHTVRKKSADGTGPTPLD
                     IEKTCARCHVDVDAAFSKSAHGKALARGVREAPTCTTCHGEHNILKAQDPSSPVAAAN
                     VAQKVCTPCHSSLRLSEKYDIKSDAFKTFEESFHGLAVRGGQANVANCASCHGSHDIL
                     PSSDPASRIHKDNLVKTCGECHPGAGARFAQGKIHVDATSKDGEPLLWWIGFVYAGLI
                     VGTIGGMAAHNLLDFVKKSRHQHHVRHGLVEVEPAPHRLYLRMTLEERLQHGALALSF
                     IVLVITGFMLRFPDSWWVVALRNLSSSAFEARSWIHRIAAIVMCLASAYHLYYVIFTA
                     RGRQLVKDLWFRLKDARDLLDMLKYNLGMTPVKPKLDRFSYIEKAEYWALIWGTIVMA
                     ATGFAMWLDNSFMNLFGKLGYDVARSVHYYEAILATLAIIVWHFYFVMFNPDSYPLNT
                     AFWTGHLSHKEMEEEHPLELERILEAEEKERAAAEMARAQAEAKDQEKPKA"
     gene            complement(19912..>21456)
                     /locus_tag="P5164_01470"
     CDS             complement(19912..>21456)
                     /locus_tag="P5164_01470"
                     /inference="COORDINATES: protein motif:HMM:NF023335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HRY42589.1"
                     /translation="YDPGELARRYVVAGGAEVRARLVLQEVERRVADPRRMRALGFCV
                     SVEHARFMAEQLGKAGIASEAVVGETPTEEREAAVRRLESGDLQALFTVDLFNEGVDI
                     PTVDTVLFLRPTESATVFLQQLGRGLRLHREKPYLTVLDFIGNARREFRFDRRFRAIL
                     GGTTRQVERQIEAGFPYLPPGCAMQLDPRAMQVVLENIRRNIGSGQRWLAQELVALGP
                     DATVGEFLRETGVEAVELYSGGAGGRSFASLRQAAFGGEPLRDEERRRHARLTGILHV
                     NDPLRLGFLADLAAGRPLPEGEREDRLAAMAAAALLDVRLPTEARGALEAARADSRYR
                     GELGQLVEYLDDSRREGVLDWRELGPAPLQLHARYRQDEILAALRTGGARGIPRLQGG
                     VYFEPASGTDVLLVTLRKSEKSFSPTTMYRDYAISPSLFHWESQNSAHPGTEAGRRFS
                     AGTSNVLLFVREVQEQANGVAEAYTFLGRVTLESWQGERPMQIVWRLAHPMPGPLYAH
                     ATVAAG"
BASE COUNT         3125 a         7806 c         7393 g         3122 t
ORIGIN      
        1 gatcatgatc tccggcttga cctcgacccc ttccttcttc acgttgcagg ccgcctcgag
       61 gatggcgcgc gcctgcatcc gcgtgatctc ggggtagacg atcccgaggc ggcagccgcg
      121 gtggccgagc atcgggttca tctcgtggag ctcgttgacc ttctgcgcca cggccgccgg
      181 cgagatgccg agctgcctgg ccacgtcctt ctgctcgtcg gccttgtgcg gcaggaactc
      241 gtggagcggc gggtcgatcg tccggatcgt caccggccgc gggcccatcg cgcggaacac
      301 cccctcgaag tcgccgcgct gcatcgggag gagcttcgcg agcgccttct cgcgggcctc
      361 gaccgtgtcg ccgaggatca tctcgcgcac cgccgtgatc cggtcgccgc cgaagaacat
      421 gtgctcggtg cggcagaggc cgatcccctc ggcgccgaac aggaccgcgt tcgtggcctg
      481 gtcgggctgg tcggcgttcg cgcggacgcg gagcctgcgg accttgtcgg cccagcccat
      541 catcgtcgcg tagagcgtgt agacggggct ctcctcgggc ttcagcgtct tctcgaggag
      601 gacctgaagg acctcggagg gcttcgtcgc gatccgcccc tcgaggacct ctcccgtcga
      661 gccgtcgatc gagagccagt cgccttccat gacgacgcgg ttcttcacgt cgccccggac
      721 ggtcatgcag cgctgggcgt agtcgatccg gagcgactcg cagccgacga tgcagacctt
      781 ccccatctgc cgcgcgacga gcgccgcgtg ggaggtcatg ccgccgcggg ccgtcaggat
      841 cccctcggcc gcgttcatcc ccttgatgtc ctcggggctc gtctcctcgc ggacgaggag
      901 gaccttctcc cccttcttcg cccactcgaa ggcgtcctgg gcggagaaga cgacgcgtcc
      961 cgtcgcggcg ccgggtccgg cgttcagccc cttcgcgagg agctgcccct cggcgacggc
     1021 ggccttcttc gcgttcgggt cgaagaccgg acggaggagc tggttcagcg agtcggggtt
     1081 gacgcggagg atcgcctcct cgggcgagat gagcttctcg cgctccatct cgacggcgat
     1141 ccgcacctcg gcgaagccgg tccgcttgcc ggtgcgcgtc tgcagcatga agagccggcc
     1201 gcgctcgacc gtgaactcca cgtcctgcat gtcgcggtag tgcttctcga gcttcgcgcg
     1261 gatccccatg agctggtcgt agaccttcgg catcgcctgc tggagcgtgg cgatcttctg
     1321 gggggtccgg atgccggcga cgacgtcctc gccctgggcg ttgatgaggt actcgccgta
     1381 gaagaggttc tcgcccgtgg cggggtcgcg cgtgaaggcg acgcccgtgc cgcagtcctc
     1441 gccgaggttg ccgaagacca tcgactggac gttgacggcc gtcccccagc tctccgggat
     1501 cgagttgagc tggcggtaga cgatcgcccg ctcgttcatc caggacgaga agaccgctcc
     1561 gacggcgccc cagagctgct tcatggggtc ttcggggaag tcgtggcccg tcttctcctt
     1621 gatcgccttc ttgaactcgc cgacgagctc cttcagcgcc tcgacggtca gctcggtgtc
     1681 gaggtggacg ccgaacttct ccttcttcgc ctcgaggatc agctcgaacg gatcgcgctc
     1741 gtgcttgccg accggcttca ggtcgaggac gacgtcgccg tacatcgcga cgaagcgccg
     1801 gtagcagtcc cacgcgaatc gcgggttgcc cgaggccgtc gcgagcccct cgaccgtcac
     1861 gtcgttcagg ccgaggttca ggatcgtgtc catcatcccc ggcatcgagg cgcgcgcgcc
     1921 cgagcggacg gagacgagga gcgggttctt cgggtcgccg aacttcttgc cgagcttctt
     1981 ctcgacgccg gcgaggtgcc gggcgacctc ggcttcgagc cccttcgggt agctccggcc
     2041 gttcgcgtag aagtgcgtgc agacctcggt cgtaatcgtg aacccggggg ggaccggaag
     2101 gccgatccgc gccatctcgg cgaggttcgc gcccttgccg ccgaggaggt tcttctggct
     2161 gccgtcgccc tcggcggacc cgccgccgaa ggagtagacg tacttcgccc cccgcgccgt
     2221 cggcgcggac ttgctggtca tggggttcct cctgagtttc ggatgctcaa tgtatgtgaa
     2281 cgttcgtctt cgaacgggca attctaccgc ctcgaggaga tcggctcccg accggcccct
     2341 cgaggggtcc tcggggcgcc ggccgcggac ggcgtctcgc cccgaggcgc tggacgcacg
     2401 cggtccgtcg gtagagcagg gagagcaacg tgacgtttcg ccacacctcg cgggtttccg
     2461 cggcgaagcc gaatgaccgt ccggtaagat gccccgcggt atgcatctcc cccaccgcgg
     2521 ggcacgtatc ccaccggtgc cccttctcct tcttctcccc ctcctcctct cgacatcggt
     2581 cctcgccgac ggcacgcccc tcaccctgga cgaggcgatc cgtctcgcgg tcacgcgaaa
     2641 cgaacgggcc ctcgcctccg gagagcggct cgaggcggcc gaggcgcgcg tctccaaggc
     2701 gcgggccttc ttcttcccgg acgtccaggc tgccgggaac tacacccgac gccccttcga
     2761 gcggacgagc accgtcggag ggcaggaggt cgtcctctcg aagcgcgacg ccttctcctc
     2821 gagcatcacg gcgaggcagg tcctcttcga cgcccgcgcc ctgccgctct ggcggcaggc
     2881 ccggagcgac cgggacgccg cgagcctcga ggccgccgag gagcgacggc tcgtcgcgtt
     2941 cgaggcggcg gccgcgttcc tcaggaccct cggcgccgac caggtcgccg gcgccgccga
     3001 gcgccgcgtc gagctggccc ggaagaacct cgaggacgcg cgtgcccggt tcgaggcgga
     3061 gatcacgagc agcaacgacg tgacgcgcgc ggagctcgag ctcgcgacgg ccgagcgcga
     3121 ggcgacgcgc gcccggaacg acgaggagac ctcgcgcctc tccctggcga acctcctcgt
     3181 ttcggacgtc cccgaggcgc tcgaggtccc gcagtccctc ctcgcgacgg ccgaccgtga
     3241 gcgcccggcg gaagaggagc tcgtcacccg ggcccgcgag cgccggctcg acgtcgccgc
     3301 agggctgaag cgcgccgagg cgctccactt ctgggcgaag gagccgatga accgcttctt
     3361 cccttccctc gtcgctcggg ccgacggccg gtggacgaac gagggcggcc tgaacgcccg
     3421 gaacacgacc tggacctacg ggctcgacct ctcctggtcg ctcttcgact ccgcgacccg
     3481 gttcgccgac cggagggagc ggaacgcgaa cgcccgcgcg gccgacctga acgcccgggc
     3541 ccgcgagcgc gccgtcgagc tcgaggtaaa ggccgccctc gcctcgctcg acaacgcccg
     3601 cgccgcggtc cggcaggccg ccgtcgcgct cgaggccgcg cggaagaacg cctccgagac
     3661 gacgacgctc taccgcgaag ggctcgcgaa cgcgctcgcc gtcgccgacg cgaaccagag
     3721 cctcttcgag gccgacgtcg cgctgacgaa cgagcgctac ggcctcggcg ccgccgtcct
     3781 cgacacctgg gccgccctcg gcctcgaccc ggcaggaaag gagcccctcc cgtgaagcgc
     3841 cccactctcg cccccgttct cctcctggtc ggaaccgccg cgctcgcctt ctcctgctcc
     3901 ggaggcggcg agaagaaggg cgcctccggc ggcaagggcg gccctccggc gttccccgtc
     3961 gagacgtcgc cggtgaggag ccgccccgtc gagtacacgg tcacggccgt cggctccgtc
     4021 gaggcgttcg aaaaggtcca ggtcacggcc cgcgtcgccg gcgtcgtgga gcaggtgaag
     4081 ttcgtcgagg gagagtcggt cgagaccggg acggcgctcg tcgagatcga gccgcgccgc
     4141 taccagctcg ccgtcgaggc cgcgcgcgcg aacgtccaga aggccgaggc cgcgaaggcc
     4201 gacgccgagg ccgccctggc gcgacggcga gccgtcgtcg agaagaaccc cggcctcatc
     4261 ccgggcgagg agatcgacac gttcgccacg cgggtccaga ccgccgcggc cgacgtcgcc
     4321 caggcgaagt cggcgctcga ccaggccgag ctgaacctgc gcgacgcgtt cgtccgcgcc
     4381 cccgtcgccg ggcgcatcga gacgcggacg gtcacgaccg gccagtacgt ggctcccggg
     4441 ggcgtcctcg cgacgctcgt ccggcgcgac ccgctcctcc tccgattcca tgtcccgtcc
     4501 gaagaagccc ggagccttcg ccccgggctc gtcgcgcgct tccgcgtccc cggcgacgcg
     4561 aaggagttca cggcacggat cacgcacgtc gccggcgccg ccgaggcggc ctcgcggatg
     4621 gtcgccgtca ccgcccaggt cgacgacccg cggcgcgccc agctccgccc gggcgcgttc
     4681 gccgacgtct ccgtccccgt cggcggcatc gccgactccc ccgtcatccc gcagacggcg
     4741 atccggccga gcgagaaggg gttcctcgcc ttcgtcgtag aggacgggaa ggctcgcgag
     4801 cgcgtcctca ccctgggcct ccgcacgacc gaagggctcg tcgaggtccg cggagggctg
     4861 aaggcgggcg aggagctcgt catccgcggc gccgaggcgc tccgggacgg cctccccgtc
     4921 aaagtgagcg ctcctggcgc cgagccgacc gccccgtcca ggccggaggc gcgcggatga
     4981 acatcacgga ggtctgcatc cggaggcccg tcctcgcctg gatgctgatg gccgcgacga
     5041 tcgtcttcgg cggcgtcgcc ctctcgcgca tcggcatcag ccagttcccc gacgtcgact
     5101 cgccgacgat ctccgtctcc gtgacctggg aaggggccgc cccggaggtc atggagcacg
     5161 acgtcgtcga gccgctcgag gaggccgtga tgcaggtgga ggggatcaag tccctcacct
     5221 cctcctcgag gcagggctcg gcgaacgtga cgatcgagct ggacatcagc cgggacatcg
     5281 acctcgcgct ccaggacgtc cagacgaagg tctcccaggc gcagcggtcg ctcccccgcg
     5341 acatcgaccc tcccgtcatc tcgaagtcga accccgagga caacccgatc ctctggatcg
     5401 gcctgtccgg cccctttccc cgtcaggtca tcgccgacta cgcccgctac cgcctcaagg
     5461 agcgattcca gaccgtcccc ggcgtcggcg aggtgaacat ggggggctac ctcgaccgga
     5521 acgtgaggat ctgggtcgac gccgacaagc tgaactccaa gggaatgacg gtcaccgacg
     5581 tcatcgcggc gctccagcgc gagcacgtcg agctcccggc cggccgcctc gagacggaag
     5641 gccgcgagat cagcgtccgg gtcatgggcg aggcgctcga cctcgccacg ctccgcaaga
     5701 tatcgctccg cgacctgaac ggtgcgccgg tctacctcga ggacgtcgcc ctcgtcgagg
     5761 acggcttcga ggacgagcgg cgcatcgcac gcgtcaacgg ggcccccgcg cagggcctcg
     5821 gaatccgcaa gcagcgcggg tcgaacgccg tcgccgtcgc tcagggcgtc aagcaggcga
     5881 tggccgagat ccagaagacc ctccctccgg ggatggaggt cgggatcaac ttcgactcga
     5941 cgacgttcat cgaggactcg gtgcacgaga tccagttcga gatcctcctc tcggtcgtcc
     6001 tgacgggcct ggtctgctgg atgttcctcg gctccctctc gtcgaccctc aacgtcgtcc
     6061 tcgcgatccc gatgtccctc ctcgggacga tcgcgttcat ctacttcgcc ggcttcacgc
     6121 tgaacacgtt cacgctcctc gcgctcgggc tcgcggtcgg gatcgtcgtc gacgacgcga
     6181 tcatggtcat ggagaacatc ttccggcact tcgaggaagg gaaggaccgg gtgacggccg
     6241 cgcgcgaagg gaccggcgag atcacgttcg cggccctctc ggcgacgatc gcgatcatcg
     6301 cgatcttcat cccggtcgtc ttcatgaagg gggtcatcgg gaagttcttc ctccagttcg
     6361 ggatcacgct ctgcgtcgcg gtcgccctct cctacgtcga ggcgatcacg ctcgcgcccg
     6421 cccggtgcgc gcagatcctg aaggcgggcg aggtgaaccg gagcggtctc ggcggcctcg
     6481 tcgaccgcgc tttcgacgcc ctcgcacgcg tctacgcccg cgtcctcgcg aaggggctga
     6541 agcaccccgt cgcggtcctc gtctcggcca cccttctcct cggcgtcgcg ttcctcgtct
     6601 tccgcgcgct cccctcggag ttcgtcccgt cgcaggacca ggggcgcctc atgctccgcg
     6661 tccagacggc cgtcgggtcc gacatccgcg agacggacat ggccttccgg aagatcgaga
     6721 actacgtcac gtcccgtccc gaggtgacgc gctgcttcgg ggtcgtcggc ggattcggcg
     6781 gcggcgtgag cggcggcgtc gtcttcgtct cgatggtcga caagagcgac cggaagctgt
     6841 cgcaggccga gttcgcggcg gagatccgga aggagctcaa ctcgtacccc ggcgtgaggg
     6901 tcgtcgtcca ggacctctcg cagtcgggct tcacggccca gcgcgggttc ccggtcgagt
     6961 tctcggtccg cggccccgac tgggacgtcc tcgtcgagaa ggcgcgggga atctcggaga
     7021 ggctgaacgc gagcggacag gtcgtcgacc tcgacatgga ctaccggatc gggatgcccg
     7081 agctccggat cgttccgaac cgcgcccgtg ccgccgacct cggcatctcc gtcgagaacg
     7141 tcgcgacggc cctcaacgcg acgctcggcg gcctgcgcat cgggaagtac tcgacggagg
     7201 ggcggcgaat cgacgtccgc ctccgcctcc tcgccggcca gcggacccgg ccggaggacc
     7261 tctcgcgcct tcgcctccgg agctcgaccg gcgacctgat ccctctctcc tctctcgtga
     7321 cgacggagga ggtccccgct ctgcaggcga tcacgcggcg cgaccgcgag cgggcgatca
     7381 cgatctacgc gaacgtggcg ccggggatgt cgcaggcgga cgtcctcaag tacgtcgagg
     7441 ggctcggttc cgacatgccg accggctacc gcctcgtcct cggcggcgcg agcgtcgcct
     7501 tccgcgagtc gatgggcgac ctcctcttcg ccctcgtcct cgggatcggc gtcgcctaca
     7561 tggtcctcgc ctcccagttc aactcgttcc tccacccggt cacggtcctc tcgatcctcc
     7621 ccctctcggt cgcgggggcg gcgttcgccc tcctcgcgac gggccggagc ctgaacatct
     7681 tctcgatgat cgggctcctc ctcctgatgg ggatcgtgaa gaagaactcg atcatcctcg
     7741 tcgactacac gaaccagctc cgggagcgcg gcctcgacac gctgagcgcg ctgctcaagg
     7801 cggggccggt tcgcctccgg ccgattctga tgacgtcgat cgcgacgatg atggcggccg
     7861 tcccgcccgc cgtcgggatc ggctcgggct ccgagatccg ggcgccgatg ggggtcgccg
     7921 tcatcggcgg cctcatcgtg gcgacggtcc tctcgctcct cgtcgtgccg tcgttctacc
     7981 tcgtcacgga ccggattcgg gaccgggtgc agggccgccg ccgcgagccg gccaccccgt
     8041 cggagccgtc ggcgtccgca ccctgacgcc ctctcgcagg cgagacgatc acgcggcggc
     8101 ggccttcacg gccgtcgccg cgtggcggcg cgtctctgct atggtccccc gcccatgatc
     8161 gccgtctcgg atctcggcaa ggccttcggg gcgcagaccc tcttcgaggg ggtctcgatc
     8221 cagttcaacc ccggcaaccg gtacgggctc gtcggggcga acggctccgg gaagtcgacg
     8281 ctcctgcgca tcctctcggg cgaggagtcc ccgtcgagcg gcgtcgtctc gattccccgg
     8341 aagctgacgc tcggcgtcct ttcccaggac cactaccgct acgaggagat gccgatcctc
     8401 gacgtggcga tgatggggaa ccgggaagtc tgggaggcga tggcggagaa ggagcggctc
     8461 ctccacgccg actccgagtt ccacggcgac cggtacgccg agctcgagga cctcatcctg
     8521 cggaaccagg ggtactcgct cgaggcgcgg gcgggcgaga tcctggaggg cctcgggatc
     8581 ccgacggagg tccaccggtc gccgctctcg acgctctccg gcgggttcaa gctgcgcgtc
     8641 ctcctcgggc aggtcctcgc ggccgacccg ggcgcgctcc tcctcgacga gccgacgaac
     8701 cacctcgaca tcgtctcgat ccgctggctc gagaagttcc tcgtctccta caagggcctc
     8761 gcgatcgtca tctcgcacga cctccgcttc ctcgacaacg tctgcaccca catcgccgac
     8821 gtcgactacg agacggtgac gctctatccg gggaactaca ccgcgttcga ggccgcgaag
     8881 gaggcggagc gggagcggaa ggaagccgag atccagaagc gcgagaagca gatcgccggg
     8941 caccaggcct tcgtcgaccg cttccgcgcg aaggcgacga aggcgcgcca ggcgcaaagc
     9001 aagatcaagc tcatcgagaa gatcgtcatc gacaagctcc cggagagctc ccggcggcac
     9061 ccgcgcttcc ggttccgcca gcggcggccg agcgggcgga ccgccctcga gatcgaatcc
     9121 gtcggcaagg cctacgggga caacgtcgtc ctgaaggacg tctcgctccg ggtcgagcgg
     9181 ggcgaccgcc tcgcgatcat cgggccgaac ggcatcggca agtcgacgct cctgaagatc
     9241 gccgtcggcg agctcgctcc cgacgccggc aaggtgacgt ggggctacga agcgagcccc
     9301 ggctacttct cgcaggacca cgccgagctc gccggcgcgg gacggcagtc gatcgaggcg
     9361 tggctctggg aggcggcgcc gggcgagccg atcggcttcg tgcgggggca cctcggccac
     9421 gtcctcttca cgggcgacga cgcggagaag cccgtccggg ccctgtccgg gggagaggcc
     9481 gcgcgcctcg tcttcgcgcg cctctcggtt ctcgggccga acgtcctcgt cctcgacgag
     9541 ccgacgaacc acctcgacat cgaggcgatc gaggccctcg tcacggcgct cctcgactac
     9601 gacgggacgc tcgtcttcgt ctcccacgac cgctggttcg tctcgcgcct cgcgaaccgc
     9661 atcctcgaga tctcgccgaa ggggctcaac gacttccgcg gcacgtacga cgagtacctc
     9721 gagcggtgcg gcgacgacca cctcgacgcc gaagccgtcc tccagagcct ccggaaggcc
     9781 cggaagagcc aggacgcgag gaagaaggag cccgagccga agcgccagaa ccgggtgaag
     9841 gcgctcgaga ggaggcgcga cgagctgacg agcctcatcg aggccgccga gtctcgagtc
     9901 cacgcgatca gcgagcggtt cctcgacccg gccctcttcg gaaagggcgc ccaggccgag
     9961 acgcgaaagc tcgaagagga gcagaagcag ctgagggccc ggatcgaagg gctgatgtcc
    10021 gagtgggagc aggccgagag ggagctcgag gcggagcgcc cgggcgcgta ggtctcagct
    10081 ccggtagtcc gcgttctccg tcacgtactc gtgcccgagg tccgccgcga ggagacgcgc
    10141 ccgcgccttc ccgacgccga gatccgcgac gagggggacc cgctcgcggg cgaacgcctt
    10201 cgccgccgcc gcctcgcggt agccggtcgg cgcgccgtcc tcgacgacgg agagacgacc
    10261 gatcctcagc gagacgcgac gcgtcgagac gcgcgcgccg cttcggccga tcgcggcgag
    10321 gatgcggccc cagttcgcgt cgccgccgta gagggccgtc ttcacgagct gcgaggtcgc
    10381 caccgcgtgc gccgcgagct tcgcctcgcg ctcggtggcg gctccctcga tcgtgatctc
    10441 catgacccgc cgcgcccctt ccccgtcgcg gacgatccgc caggcgagct cgtcgcagac
    10501 cttcgcgagg gcggcgcgga acgccccagc cccgtcgatg gaccggagct cccgcccgcc
    10561 cgaggcgccc gaggcgagga ggaggaccgt gtcgttcgtc gaggtgtcgc cgtcgaccga
    10621 gatcgcgttg aagctcccgt cgaccgcctc cttcagagcc gcctgaagga gcgccggcga
    10681 ggccggcgcg tcggtcgtga cgaaggcgag catcgtcgcc atgttcgggt ggatcatccc
    10741 ggcgcccttg gcgacgccga ggacgcgaca ggtcttcccg ccgtgacgga acgtcgcccg
    10801 cgcgaccttc ggggccgcgt ccgtcgtcag gatcgcgcgg gagacggcct cgatgccgcc
    10861 ggaggagagc cgcgcgaacg cgtcggggag gaacgtcgtg accttcgcgt cggggaggac
    10921 gactccgatc actccggtcg aggcgacgaa gacctcctcg cgggggcagc cgacgaggtc
    10981 ggccgcgcgc tcctggaccc gccgggccgc cgcgagcccc tccgcgcccg tgaccgcgtt
    11041 cgcgcagccg gcgttcacca ccacggcctt cacgcgcccg cggctctttc tcagggaggc
    11101 gcgcgagagg aggaccggcg ccgcctggaa gcggttcctc gtgaagacgg cggccgcgct
    11161 cgcacctccg ggggcgacga ggagggccac gtcgaggccc ggcttcttcc ggagtccggc
    11221 tgcgacggcc gatccgagga agcccttcgg caggttcatc ggacgctcct tcccgctgcg
    11281 gccttctgcg ccgccgcgat ccgcttcgcg gcccttcttg ccgcctgcgc cacgcgcttc
    11341 ggcgccgttc cgcccaccgc cccgcggcgt gccagggccc gtccgaggtc gaccgaggcg
    11401 aggtccgcct cggtgactcc gtccgaaggt gtcctctcga ggagcccctg gcccgatgcc
    11461 tccgccgccg cgaagcgccg gccggcgatc tcgtgggcct gccggaacgg gacgcctcgt
    11521 cgcgcgagcg cgtcggcgag ctccgtcgcc aggagccggt cgtcggtcgc ggcgttgcgc
    11581 atccgctcgc ggcggagctt caggccggcg acgagcgccg tcatcgccgg gagcgccgcg
    11641 tcgaggacgc cgcggacccg gaagagcggc tccttgtcga gctgcaggtc cttgtcgtag
    11701 gcgagcggga gtcccttgag gacggcgagg aggccggtga gctcgccgac cgcgcgcccg
    11761 gcgtgcccgc gagcgagctc gaggaggtcg gggttcttct tctgcggcat tcgcgaggag
    11821 ccggtggcga gggcgtccgg cagctcggcg aaacccgcct cgtccgacgt gaagaagatc
    11881 aggtcctcgg ccatccggga gaggtgcgaa aggtggagag cggcgacgaa gaggtactcg
    11941 acggcggcgt cgcggtcgga gacgccgtcg agggagctcg ccgtcgcgcg ctcgaagccg
    12001 aggctccgcg cgagccgctc gcggtcgatc gcgagaggcg tgccggagag ggcgcccgag
    12061 ccgagcgggc attcgttcgc acgcttccgc gccgcgcgca cgcggtccgc gtcgcgcagg
    12121 agcatctcca cgtaggcgag gcaccagtga ccgaacgtca ccggctcggc ccgcttgctg
    12181 tgcgtgtacc cgggcatcgg cgtggccgcc tcctccgagg cccgcgtggc gagggccgcg
    12241 gcgaggtcga ggagcgcggc gtggagcgcg tcgaacgcgc tccgcagcca gaggcggagg
    12301 tcggtcgcga cctggtcgtt ccgcgagcgg cccgtgtgga gcttcccggc gaggggaccg
    12361 atccgcttcc ccagctcccc ctcgacgtac gagtggacgt cttcggcgtc ggagtcgcgc
    12421 ccctcgcccg cctcccaggc cgcgagcacg tcccggaggc ccttcacgat cttccgggcc
    12481 tccgtcgccg agaggacgcc gcagcgcccg agctcctcgg cccaggcgat cgagccggcg
    12541 acgtcctcgg cgatcagcgc ccgatcgaac gaccacgagt cgttgaaccg gcggaggacc
    12601 tccgccggag aggcggagaa gcgccctccc cagaggctgt gccgcgggct catccccacg
    12661 gctcgaggcg gagcttctcg cccgccttca ccgcggcgtc cgtcggcggc acgagccgct
    12721 tgcggccccg cgtcgggagc ccgaagaggc gaatgaagcc cgtagcgtcg tgggccgtat
    12781 agcccgtcat gttgaacgag gcgaggttcg ccgagtagag cgagtgctcg gagcggcggc
    12841 cgacgacgat cgccgagccc ttgaagagct tcagcgtgac gtgccccgtg acggtctcgg
    12901 tgaccttctc catgaacgcc tcgagcgcct cgcggatcgg gctgaaccac tgcccgtagt
    12961 agacgagctc ggcgtagcgg agggcaaggc gctccttctc gtgcatcgcg tcgcggtcga
    13021 gcgtgagcgt ctcgagggcg cggagagcct ggacgaggag cgtcccgccc ggggtctcgt
    13081 agacgccgcg cgacttgatg ccgacgaggc ggttctcgac catgtcgatc cgcccgacac
    13141 cgtgccgcgc cgcgatcgcg ttcagcgtga cgaggagctc ggcggccggg aggagcttcc
    13201 cgtttacctc gaccgggtag cctccctcga agccgatcgt cacccgctcc ggctcgtcgg
    13261 gagccttccg cgggtcgacc gtcagcttga acatcgactc ctcgggctcg aaggccgggt
    13321 cctcgagcgg ccccccctcg tgcgagatgt gccagaggtt ccggtcgcgc gagtagggct
    13381 ccttcttcgt caccgggacc gggatgttcc gcgcggcggc gtagtcgatg gcgtcctcgc
    13441 gcgagttgat cgtccactcg cgccagggcg cgacgatcgt cagctccggg gcgagcgcct
    13501 ggtaggcgag ctcgaagcgg acctggtcgt tccccttccc cgtgcagccg tgggagaggg
    13561 cgtcgcatcc ggtcgcgagc gcgatctcga cctgcttctt cgcgatgatc gggcgcgccg
    13621 tggaggtgcc gaggaggtac tcgtgctcgt agaccgcgcc ggcgcggagg accggccaga
    13681 ggtactcggc cgcgaactcc tcccgcgcgt cgatcaggtg gaagtcgcat gcgccggtcg
    13741 cgagggcctt ctccttcagc ccggacgtct cctcggcctg gccgacgtcg accgccaccg
    13801 ccacgacctc gcagccgtag ttctccttga gccaggggat gatgattgac gtgtcgaggc
    13861 cccccgagta ggcgagggcg gccttcttga tcttcgtcgc gctcatgcgg caccttcctt
    13921 gaggatctgt tcgagtgtgg tcaggaaggc cgagagggcg gccttcggaa cgacgtacgg
    13981 cgggaggagg cggatcacgt tcttccgggc cgtgccgacg acgaagccct tcgcgaggag
    14041 cttcttctgg acggggccgg cttcgcggtc gagctcgatg ccccggatga ggccgtcgcc
    14101 gcggacgtcg aggacggccg ggcagcgctt cgagagctga gccagcttct tcccgaacca
    14161 ctcgccaatc tcctccacct tcgcgacgag ccccccctcg acgagctcct cgaggacggc
    14221 gaggccgagc cggcaggcga cggggttgcc gccgaacgtc gtcccgtggt ggcccggctt
    14281 cacgagcgag gcgacccgct cgttcaggag gacggcgccg aggggcagcc cgccgccgag
    14341 gggcttggcg agcgtgacga cgtcggggac gacgcccgtc cgctggaacg cgaagagctt
    14401 ccccgtccgg ccgaggccgc actggatctc gtcgaagacg aggacggcgc ccgccgcgtc
    14461 gcagagctcg cgcgcggcgc ggaggtactc gggcgccagc gggacgattc ccccttcgcc
    14521 ctggnnnnnn nnnncgaggc cgtgtcgtcg accgccgcgc ggagggcgtt gacgtcgttc
    14581 gccgggagga acgtcacgcc cggcaggagc ggctcgaagg gctcgcggta gggcgggttg
    14641 ccggtgagcg aaactgcccc gaccgtccgc ccgtggaacg agttgtcgag cgcgacgagc
    14701 ttcgaacgcc ccgggttcgc gagccgcgcg aacttgatcg cggcctcgtt cgcctccgtc
    14761 ccgctgttgc agaagaacgc gcgagagagc cccgagagcg tcacgagccg ctcggcgagc
    14821 tcgccctgcg ccggctggta gaagaggttc gagacgtgga ggaggtccgt cgcctcggcc
    14881 ttcagcgtct tcacgaggcg cctgtggcgg tagccgaggg cgttcaccgc gatgccggcg
    14941 aggaggtccc agtagacctt cccctcggcg tcgacgagct tcgccccgcg cccgtcgcgc
    15001 gggtggaacg gggtccagga gtaggtgccg agaagatacg cggccgcgcg ggcctccacg
    15061 gcggcggggc tttcgtcagg ccgggacaag gcaggtccct cccttcccct cgaggagggc
    15121 cgcgcggtgg agcgacggcc ccgcgacgag gacctcggag acgccggcgt cgagtgcgtc
    15181 gagcgcggcg cggagcttcg gcttcatgcc gcccgtcacg gcgggcgagg cgaggagcgc
    15241 gcggccggcg cgcgcgtcga gctgctcgat caccttcccg tcggcgtcga ggaccccctc
    15301 gacgtccgtc aggaagacga gccgcttcgc gcccagcgcc ccggcgaggg ccgaggcggc
    15361 ggaatcggcg ttcacgttca gtagggtccc ctcgcgcccg agcgcgaccg acgccacgag
    15421 cgggagaaac gaggcactga ggacggccga caggagcttc gggtcgcagc ggacgacgcg
    15481 gccgacgaag ccgaggtcga ccccctcgac cgcctcgtgg aactcggccc agagcgtgtc
    15541 gccgtcggcg cccgagatcc ccgaggcgag gatccccatc gagcggagct cggcgacgag
    15601 cgtcttgttg acgagccccg agaggatcga cacgacgacc tcgagcgtct ccgcgtcggt
    15661 gacgcgcagc cccccctgga ccttcttcgg gatgtcgagg gccttgagca tcgcgtcgat
    15721 ccgcttgccc ccgccgtgga cgacgatgga gcgctttccc gagctccagg ccgtcgcgac
    15781 agcctcgagg gccgagtggc gcagcgcctt gttctcgagg aggctccccc ccagcttgac
    15841 gacgtcgatc aaagaagtcc ctccgtttcg ggcattccga agagccggtt gaggttctgg
    15901 accgcctggg aggcagcccc cttgaggagg ttgtcgatcg ccgagacgac gaccgcgcgc
    15961 cggcctccgg gcaggagctt ccagccgacg tgggcccgcg gggtcccgac gacctccttc
    16021 agctcgggga ggcgccccgc ggacaagacc ttcacgaacg gcttccccgc gtaggctgcc
    16081 gcgtatctct ccgcgatcgc cgcgtcggac acggcggcgt cgaacgagac gtggatcgtc
    16141 gagaggattc cgcggacgag cgggaggagg tgcggcacga acgcgaacgc cgcctcggac
    16201 gcgagcccga gctcctgccg catctccggc tcgtgccggt gcgtcccgac gccgtaggcc
    16261 ctgtagcttc cggcgacctc gcagaacgag tacgcaaggt cggccttctt tcccgccccg
    16321 gagacgccgc tcgccgaggt gacgaggacc ggttgtgaag ggtcgaggag cgacgcgagc
    16381 ggtttgaggg ccacgaggac cgacgtcggg tagcagccgg ggttcgcgac gagcttcgct
    16441 ccctcgagct cttccccgca ccactccgtg agtccgtaga cggcctcggt caggagcgac
    16501 ggagccgggt gcgtgaagcc gtaccacgcc gggtagagag aggcgtcctt cagccgaaac
    16561 gcgcccgaga cgtcgatcac gcggacgccc gccgcgagga ggcggggcgc gacctccgcg
    16621 gaaacctcgt tcggcgtcgc gaggaccgcg gcgtcggggc gacacgaaag gagcacctcc
    16681 tccgagaacg gaacgacgtc ggggcccgag gctccgccga ggtcggagtg cagatcctgg
    16741 aagggcgctc ccttccctcc cgggcttccg aagagcccgg cgagctcgac gcccggatgg
    16801 cgcgacagga ggcggaccgc ctccgcgccc gagtagcccg cagcgcccac gacggagaca
    16861 cgaatcactt tcaagtcctc gattctccgg aacggcgtcc ggactctagg cgtggcgtcg
    16921 gcggtccgcc gaaggcgggc cggcgagtcg ggcggccagg gcgcgcgcgg agacggcacc
    16981 gggggtcgcg acgaagaccg tgtcgtcgcc ggcgatcgtc ccgatcgcct ccgcgagctc
    17041 cgcctcgtcg acggcccggg ccaccggatg cgcgccggcc ggcggcgtcc ggaggacgac
    17101 gagagagccg gcgacgcgga cggacaagac cgcctccgac agcgtccgct gcagccgcga
    17161 ggcgcgctgc gccacgtccg ccgcccggtc gtgggtcccc tgcggagcgt agcctcccgg
    17221 ggtcttcgcg aggccgagct cgcggacgtc cctggagagg gtcggctgcg tcgcctcgaa
    17281 gcctctctcg gccaggagac gctgcagctc ctcctgcgac cggacgacct cttcccgcac
    17341 cagccggagg atggcgtcgc ggcgccggag gaccgaggcg ttggaatgat tattcggcac
    17401 gacgaaatat tattcaccga acggccgcga cgtcaaggcc accttcccgg gggtgcggag
    17461 cgcaggacgt ccgggcgctt cgccccgctt cccgccgctt ccgcctcttc ccacgactcc
    17521 ccgcgggcga aagtgtccgg cgtgtcgctt cgccgcgaga ccacatctca ggtccaggcc
    17581 caactgatgc ctctgaatca ttgaagaggc ccctcccggt ccgaataatc cgcgcccggg
    17641 ggtactcgtg accgacagca cccgatccga gcttttcgac cggcttcgtg ccggaatcgt
    17701 ccttctggtc gtcctcgcgg cctctttcgc cctcgcggcg acggcctcgg cgaagccgcc
    17761 gacggacgat gactgcctcg cctgccactc cgagccggcg ggcaagggaa agaaggcgcc
    17821 tcagaaggcg ctctccgtct ccgtccacgg cgaggcgggg ctggcgtgcg tcgactgcca
    17881 cgcggacctg gcgaagctca aggagcttcc gcacggcccc aaggtggcgc ccgccgactg
    17941 cacgacctgc cacgactccg tcgccgagag tcacgccttc cacccgatga tgaaggacga
    18001 cccgacggcg atcgcgtgcg ccgactgcca cggcgggcac gacgtcgccc gcgtcgcgga
    18061 cgcggggtcg ccgctggccg gtgcggcggt gcgcaaggtc tgcgccgcgt gtcacgacac
    18121 cgaggtcggg ctctacgccg actccgccca cggccgcgcc gccatggctg gagtccagga
    18181 agcccccgcc tgcctcgcct gccacaagac ggcgctcgtc tccgtccccg acggcgcccc
    18241 gcccgcgagc gccaagctgg cccaggagaa gctctgcctc tcgtgccacc tcgacaaccc
    18301 ggacgtgcgc tcgcgcgtct ctcccagcgc cggcttcatc gcgaagtacg aggagagcgt
    18361 ccacggcagg gccctcctcg cgggcaacga gaaggccgcg aactgcgtcg actgccacgg
    18421 agcccatacc gtccggaaga agtccgcgga cggcaccgga ccgaccccgc tcgacatcga
    18481 gaagacctgc gcccgctgcc acgtcgacgt cgacgcggcc ttctcgaaga gcgcccacgg
    18541 aaaagccctc gcccgcgggg tccgcgaggc gccgacctgc acgacctgcc acggcgagca
    18601 caacatcctg aaggcgcagg acccgagctc ccccgtcgcc gccgcgaacg tcgcccagaa
    18661 ggtctgcacc ccctgccact cctcgctccg cctttctgag aagtacgaca tcaagagcga
    18721 cgccttcaag accttcgagg agagcttcca cggcctcgcg gtgaggggcg gtcaggcgaa
    18781 cgtcgcgaac tgcgcgagct gccacggctc gcacgacatc ctcccctcct ccgaccccgc
    18841 ctcccggatc cacaaggaca acctcgtgaa gacctgcggc gagtgccacc cgggcgccgg
    18901 tgcgcgcttc gcgcagggga agatccacgt ggacgccacc tcgaaggacg gggaaccgct
    18961 cctctggtgg atcggcttcg tctacgcggg cctcatcgtc gggacgatcg gcggcatggc
    19021 ggcccacaac ctcctcgact tcgtgaagaa gtcgcggcac cagcaccacg tccggcacgg
    19081 cctcgtcgag gtcgagccgg cgccgcaccg cctctacctg cgcatgacgc tcgaggagcg
    19141 cctccagcac ggggcgctcg ccctcagctt catcgtcctc gtcatcaccg gcttcatgct
    19201 ccggttcccc gactcctggt gggtcgtcgc gctgcgcaac ctctcctcgt cggccttcga
    19261 ggcgcggagc tggatccacc gcatcgccgc gatcgtcatg tgcctcgcgt cggcctacca
    19321 cctctactac gtgatcttca cggcgcgcgg gcggcagctc gtcaaggacc tctggttccg
    19381 cctcaaggac gcccgggacc tcctcgacat gctgaagtac aacctcggaa tgaccccggt
    19441 gaagccgaag ctcgaccgct tcagctacat cgagaaggcc gagtactggg ccctcatctg
    19501 ggggacgatc gtcatggccg ccacgggctt cgcgatgtgg ctcgacaaca gcttcatgaa
    19561 cctcttcggg aagctcggct acgacgtcgc ccggtcggtc cactactacg aggcgatcct
    19621 cgcgacgctc gccatcatcg tctggcactt ctacttcgtg atgttcaacc ccgactccta
    19681 cccgctgaac accgccttct ggaccggcca cctctcccac aaggagatgg aggaggagca
    19741 cccgctcgag ctcgagcgga tcctcgaggc cgaggagaag gagcgggcgg cggcggagat
    19801 ggccagggcg caggccgagg cgaaggacca ggagaagccg aaggcctgac gttccccgtt
    19861 ccggctcgaa caccgaaggg cccggtccgc gaggaccggg cccttcttct ttcagcctgc
    19921 cgcgacagtc gcgtgggcgt agagggggcc cggcatcgga tgggcgaggc gccagacgat
    19981 ctgcatcggg cgctcgccct gccacgattc gagagtcacc cgtcccagga acgtgtaggc
    20041 ctccgccacg ccgttcgcct gctcctgcac ctctcgaacg aagagaagga cgttgctcgt
    20101 gccagcgctg aagcgcctgc cggcctccgt gcccggatgc gccgaattct ggctctccca
    20161 gtggaaaaga gacggcgaga tcgcgtagtc gcggtacatc gtcgtgggag aaaagctctt
    20221 ctccgacttc cggagcgtga cgagaaggac gtcggtgccg ctcgcgggct cgaagtacac
    20281 gcccccctgg aggcgcggaa tgccccgggc cccgcccgtc cgcaacgccg cgaggatctc
    20341 gtcctgccgg taccgcgcgt gaagctgaag gggcgccggc cccagctcgc gccagtcgag
    20401 taccccttca cggcgactgt cgtcgaggta ctcgacgagt tgccccaact ccccccggta
    20461 tcgcgaatcg gcgcgggccg cctcgagcgc cccgcgcgcc tcggtcggca gacgcacgtc
    20521 caggagcgca gccgccgcca tcgccgcgag cctgtcctcg cgctccccct cgggcaaagg
    20581 ccttccagcg gcgaggtccg cgaggaaccc gagccggagg gggtcgttca cgtgcaggat
    20641 tccggtgagc cgcgcatggc gccggcgttc ctcatcacgg aggggctccc cgccgaacgc
    20701 ggcctgacgg agcgaggcga agctccgccc gccggcaccg ccggaataga gctccacggc
    20761 ctcgaccccg gtttcgcgca ggaactcgcc gacggtcgcg tccggcccga gcgccaccag
    20821 ctcctgcgcc agccatcgct ggcccgagcc gatgttccgg cggatgttct cgaggacgac
    20881 ctgcatcgcc ctcgggtcga gctgcatcgc gcagccgggc ggcaggtacg ggaaccccgc
    20941 ctcgatctgc cgctccacct gccgtgtcgt cccgccgaga atcgcgcgga acctcctgtc
    21001 gaagcggaac tcgcgccggg cgttcccgat gaagtcgagg acggtcaggt acggcttctc
    21061 ccggtggagg cgcaggccgc gaccgagctg ctgcaggaag accgtcgcgc tctcggtggg
    21121 acggaggaag aggacggtgt cgaccgtcgg gatgtcgacc ccctcgttga agaggtcgac
    21181 cgtgaagagc gcctgcaggt cgcccgactc gagccgtcgg acggccgcct cccgctcctc
    21241 cgtcggcgtc tcgccgacga ccgcctcgct cgcgattccc gccttcccga gctgctcggc
    21301 catgaagcgg gcgtgctcca ccgagacgca gaacccgagc gcccgcatcc gccgcgggtc
    21361 ggcgacgcgc cgctcgacct cctggaggac gagccgcgct cgcacttccg cgccgccggc
    21421 cacgacgtag cgccgcgcca gctcccccgg gtcgta
//
LOCUS       DAOTEZ010000015        21444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_1618_length_21444_cov_6.414886, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000015 DAOTEZ010000000
VERSION     DAOTEZ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 21444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21444
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1618_length_21444_cov_6.414886"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="P5164_01475"
     CDS             complement(<1..647)
                     /locus_tag="P5164_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRY42590.1"
                     /translation="MKNPLWLAASALLVAAPLLSAPPATKAEEAKRFHPPAQGMPAAP
                     RLAGYEKRLAMEKASPFAGLSFRNVGPELQGGRIVDLDAPRSRPGTLFVAFATGGLWR
                     TDNKGGSWTPLFDRESASALGAIAVGDAEGNVLWAGTGEANSSRTTYSGTGVFKSIDG
                     GKSWTNTGLHDAHRIGKILVDAADPDVVFAAATGPLYTDGGERGLYRTTDGGKSWT"
     gene            complement(741..2903)
                     /locus_tag="P5164_01480"
     CDS             complement(741..2903)
                     /locus_tag="P5164_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRY42591.1"
                     /translation="MTLRTKFLLYAVLPLVAIQALLAFTDLRERRTLLLRATRASMAR
                     ALKDAAAAIDAESREAVLVARTMSLAQEAGLFGRRAESAALCRRVLEERRDFVTAYVL
                     YEANADGRDARDLAELGGRVFWIDGTGRFSAAYSRDPGEPDRIVEQRTPAAEMETALY
                     YDALRRRVAGGSGERTLVSEPYRYSDRVLMVEQTSAIMVGGRFAGITGVDRALDDLDA
                     RLRELRPFETARFYLLSSAGRVVAATLEQDVRTMRAADLRLGGNGEVVAGAYVEKGGM
                     RELDEGRLAALVPAGARTDLAELLSGAKAGPGEPVVREVTDPFDGRRAFLASARVPTG
                     GWTLVMTVSEKEVLAPIDRTIRRTLLFTGLGVAAVVFLIVFLARSLATRLGRAVVVAR
                     SVADGDLTVRIEDSAPDEPGRLLRAIGEMTTRLGSLVGKVGQATDRLAATADRMRASA
                     HRQEGAVQDFSASTTEIATALRQVAATGEEVSGAVQGVRGVAAGVARLADEGRKDLSA
                     MEAGLAQLSRSTATVSVRLGAIRERTEKITSMVSTIVAVADRTNLLSLNAAIEAEKAG
                     AAGAGFAVVAQEIRRLADQTAIATLDIEKNVAEMQGAVAEGLVEMDQLSAEMARGSAG
                     LGRLTRQMERALEEAESLHPKIASVTADVSAQAEGVGQITDGVGALAVAARQAAQEIQ
                     GFRHAEEELGRAVALLRDEVARFTVEAGPGTGGPPPTP"
     gene            3074..3457
                     /locus_tag="P5164_01485"
     CDS             3074..3457
                     /locus_tag="P5164_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRY42592.1"
                     /translation="MALSIDIQRPGDALWTARVTLAGSLDGTTAPRLEEALKPLLGAV
                     RHVVFDLADLKFISSAGIRVLLAARKELKARDGSFAMHRLQPQIVKVFEIIGALPGFD
                     IFTSEKELDDYLAAMQRKHASPGVL"
     gene            3555..5729
                     /locus_tag="P5164_01490"
     CDS             3555..5729
                     /locus_tag="P5164_01490"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HRY42593.1"
                     /translation="MRRFGLRTKLVALALAAAALPVLAFVTLTAAGRRSASREIAVEL
                     DAVARMNLGQYVRDLYAACGTVDALLAPRLWQGQSQLQLALRNAGGLSWDGPLVRWEV
                     PGAPGGLVAAPQVRVGGRPLSRERSFQVPEPAVDALARDSAILAILYQRLDERGDMLA
                     VASSVPAEDGSRLTGSLLPAATADGSPEPSVARALAGSGHRAMARLGGRMYLTSTEPI
                     RDESGRVTGMALAAVGGRELDGLYRSIVETKVGERGYFAILGAKGRQRGVYFVSKAGS
                     RDGEDIWDLRDAEGRPFVKEMIDEALRREPGTIRHNRYAWRNPDDPEAWTRVVADTYF
                     KPWDWYVNAGTNESDFLGASLRTEAAFDAILRRAALGGAIAFALAASIALLLARRLAR
                     PLAALLEETARLGRGDLRGATVPPPLPARSRDETRLLSYAFGSMAAAFASLVRQVQRS
                     GIQVVASATRIDASARQLEDSVAHQAATTPQVSASARQISATSRHLAETVDAVASVAA
                     ATAARAADGRSALSQMDGSVRLISSSSASVSRRLSAISERARSISSLVTTISRVAERT
                     NLLSLNASIEAEKAGEQGRGFAVVAREIRRLADQTAAATVDIERTVRDVLASVASGVL
                     EMDKFVDEVRVATADVERLGALLASIVEDVAGLTPRIDSVSSGTREQAEGAAQISEAM
                     SLLDRTAAASRDTLAEFRGAAESLGVAVASLRAEVERLRVDP"
     gene            5813..7981
                     /locus_tag="P5164_01495"
     CDS             5813..7981
                     /locus_tag="P5164_01495"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRY42594.1"
                     /translation="MRTRILGLAALAAAIPLPFLWLVTAAGARQASRESAAELEELGR
                     QNVRQVAGDVARLCASVNGLMQPRLDRGVAAAREILARQGGLEPDGTRRTWSAVDQES
                     RSISPVTLPGLALGGAPLVENRDPKVRSPLVDEIRETTGMWSSLFQRMNEKGDMLRVV
                     SSILTTDGRRGTGLYLPAVLPDGALNPVLASVLAGRTYRGTSLVAGRMYLAAYDPVRD
                     RSGRVVGMVAVAFDSQILEGIRRSIAGTVVGRRGRVAVLGTRGASRGVWVVSRERARD
                     GQSAWEERDAEGRPVVQELVALAGSLRPGEVAFHRSSWAGSDDAGAFRKLSGVAYFEP
                     WDWLIEAGTDEADFLGATARTDAAFDRILRRAALGAAAAFLLAAGIALLLAHRLSRPL
                     VRLLAETERLGRGDLRGAGAPSTGSSRSRDETGLLAHAFGSMAATLAALVRQVQRAGI
                     QVVASSTRIATSARRLEDSVAHQAATTPEVSASARQISATARHLAETADAVASVAAAT
                     SARAADGRAALSQMDDSVRRIEGSSASVSRRLADISDRTRSISSLVTAISRVADQTNL
                     LSLNASIEAEKAGDQGEGFAVVAREVRRLADQTAVATLDIERTVRDVQSAVSSGVAEM
                     DRFVEEVRLAGADVGRISALLSSIAEDVADLTPRLESVRSAAREQAEGAGQISEAMLL
                     LDRSASSSRETLDEFRAATRSLQDAVASLRAEVGRLQVDP"
     gene            7978..10050
                     /locus_tag="P5164_01500"
     CDS             7978..10050
                     /locus_tag="P5164_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRY42595.1"
                     /translation="MKDRLSRLPIAHRLLLSSLAFGVPIAALVAIGFLAVQQRLDTAR
                     REKLGVSLAQPLCELSGHAARHGRLASRAAAGEAVGGEAREQAARAVDAAFEALRAPG
                     ARAAAGRLGLAPEGRPDPLEELLAAWRRVPSAGSGDARSVERAHRTVLDGTAALLLRT
                     LTESRLAFDPALDSHFLAEAAFRALPAASARIAAMLAGPSPSAGERAALAFQADELGR
                     AELPRLAEALAAALHDDAGPRRSSGSDPTGLPALRDRFDASARELLSAARRAAAEASE
                     AGSVDPSADAALTSAFDLRRGVAAELDRRLDARVTRYERERLAVLLLALLAVAISARH
                     VGYIVFGISRPLASVADAAHLVAAGDVAGARERASRVPAGDGPSRNEISRLGGAVREM
                     ADGLSSLLLEVQAAGGHVKETSARIAATARELEATSSEQAASANEVSATSREIHRRAE
                     GFAATIGEVGKVVREASDLAGDGRRGLDRMESSMDQLTAATASVSERLAAISGKAEGI
                     GAIVTTIGRVAEQTNLLSFNAAIEAEKAGEAGKGFVVVAREIRRLADRTAAAAVEIGE
                     VVESMRSVVSSGVMEMDRFVDQVRREGEEVRRIGGQLGTVIEQVEALTPRFAEVLAAA
                     EAQAGSAREIREAVSQLSAAAGQTRDTLEEWSRVAQGLDGAARSLAAQVERFRLAGDG
                     AATKADGG"
     gene            10054..10515
                     /locus_tag="P5164_01505"
     CDS             10054..10515
                     /locus_tag="P5164_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRY42596.1"
                     /translation="MLYVLLSAGPRRYALAARSVVEVVPLLRLEPLPGAPPSVRGLFS
                     YRGTPVPAVDVSLLAGAGAVPERMSTRIVVVSVEEPGGRRRLAGLLATSVTETVTRSA
                     SDAPPAGLRLPEAPWIESAFVVGDEVVPCVRVEAFLPAEVRSLVDAAEGAA"
     gene            10512..11762
                     /locus_tag="P5164_01510"
     CDS             10512..11762
                     /locus_tag="P5164_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HRY42597.1"
                     /translation="MTRSVDAVESVLAARLGLDAESLSPGAVERAVARRTQALDLDGP
                     CRYLARLLEDGTEWPALVDEVVVPETWLFRDGGPFEELAAHVRSGRAPSADEPYRVLS
                     MPCASGEEAWSAAAVLAEAGLSPREALVDAVDVSRRLVETARRGVYGAPSFRSPEAEV
                     RLGARRLEDGRREVDASLRPYVRFHVENALEFEAGLPLPCYDAVFCRNLLIYLTPEAR
                     RRLTLRADRLLRPGGLMVLGHAEAFTTFFPGYVPSRRPRAFAAQKPADGVERGRVPAV
                     VPRESRGGSTPRASTRSVGPRTPAAPGTVPLSSSPEAGAALLVEARRLANAGERASVE
                     RLCVRALAADPASVDARLLLAETTLAAGDSPRAEAELERVLYLAPRNETALLLLARLR
                     DRAGDREAAARLRGRARRAARAAS"
     gene            11759..12421
                     /locus_tag="P5164_01515"
     CDS             11759..12421
                     /locus_tag="P5164_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRY42598.1"
                     /translation="MNARLLAPDDCWSRIGVSGDGSCEKLPELVHCRNCPVFSAGGRT
                     LFEREPPPGYAEEWTKVLALPKPVEAPGTLTATVFRVGEEWLALEVSHVVRVGVARPI
                     RRVPHRSGEVLLGLVNLDGELVLCASLASLLGVSDVPGAPPPGRYLAVALAGERWVFP
                     VDEIDGVHRLEQEGLGPLPSTLSEARARHARGLARTSERTVTLLDAARLLAGLRERGG
                     LS"
     gene            12418..14721
                     /locus_tag="P5164_01520"
     CDS             12418..14721
                     /locus_tag="P5164_01520"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HRY42599.1"
                     /translation="MSGLDEGLLDLFRTEAEANALALESGLVALDTGTATKEVLEPLM
                     RAAHSLKGAARIVGLDAAVVLAHAMEDVFVAAQRGKLALVPAHVDRLLAGVDVFARLA
                     SLDPSAIPARLGAMDAEIRTLVTELVAFSSAGAPAAAPAPKVDPETPDAAPPPAAASP
                     AVAPPTPLSPTSPVPAPAPPPAPSGPGEPAASREGSVVVGTGTLDRLTGLAGESLVQA
                     RRAPDLMREFATVKASLARMSGLLEDRPGGRPGERIVPVAALAREVEESRAALVRALD
                     ALERHAHRTEEIADRLHGEAVSARMRPFGEGARGFPRLVRDTARELGKEIVLEVRGEE
                     TRVDRDILALLEAPLGHALRNACDHGIELPDERLVKGKPRAGRIVLTAEHRSGALFVT
                     VADDGRGLDPERIRARVAERGLAPQSLLAGIKEAELFEFLFLPGFSTTSRVTQLSGRG
                     VGLDVVQSMARDVGGRVGLTSTLGAGTALTLQLPLTLSVVRSLLFLVSGEPFALPLSR
                     IDRVLEVPAEELRTAEGKAFVDVDGRAVGVLFARQPLGLPPVGEPGGTVKVVVVSDRL
                     DTYGLVVDSFLGERQLVLRPLDPRLGKVPDLSAAALLEDGTPVLVLDAEDLVRSIHGI
                     LSGSEVESVRPKAGSGPERRVRRILVVDDSITVREVERRLLASHGYEVDVAVDGMDGF
                     NRVRSGRYDLVVTDVDMPRMNGIEMVTRIRSESSLARLPVVIVSYKDREEDRLQGLAA
                     GASYYLTKGSFDDRSLLTAIADLIGEP"
     gene            14718..15761
                     /locus_tag="P5164_01525"
     CDS             14718..15761
                     /locus_tag="P5164_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011059482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HRY42600.1"
                     /translation="MKIRVGIAVGPGPVQEALREVVEEDPGRELAWAAPGPSEAASWC
                     AHDTPDLLLLGLDERSVEATRRILAVSPTTILIVAAADSADTGRVFDVLASGARDVVE
                     PPRRRPGGLLENEAPVRAKLALLARLAAPAASALPARTGRAEAPPAFPPLVAVGASTG
                     GPGAVGSLLAALPADLGAPIVVVQHLDDPFVKGLAGWLASRSGRSVRTAVGGERPRPG
                     DVLLAGTSAHLVVAENGTLAYTPEPRECPYRPSVDAFFSSAARSWPGKGVAVLLTGMG
                     RDGAEGLLACRSAGWLTIAQDEKTSVLYGMPRAAREIGAASEVLPLPDIAPAVARRVR
                     EERSRTRESGGIG"
     gene            15758..16960
                     /locus_tag="P5164_01530"
     CDS             15758..16960
                     /locus_tag="P5164_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRY42601.1"
                     /translation="MSNEPGNALATETGPVSPSSRPVTVLLVDDQAIVGAAVRRMLQP
                     EEGIVFHHCSDPTKAIETAESVEPTVILQDLVMPGVDGLDLVTKYRETASTRDVPIVV
                     LSTREEPETKAEAFARGANDYIVKLPDRLELVARVRYHSKGYVALLERNEATRQLMAE
                     LAEASQYVKSLLPPPLEEPRVRIDWRFLTSTSLGGDAFGYGWIDDDHLQLYLLDVCGH
                     GVGAALLSVTVMNVIRSQALPGADFRKPDEVLSALNEAFPMAKQNGSYFTIWYGVYRP
                     STRTLVHASGGHPAALLLEGPAGTAPAARLRAPNLIIGFVPGKTFRSQEVAVPPGARL
                     FLYSDGVYEVEMADGQIWPFDDFAAFMEAPPAPGASRMDELLAQARRLNRSDALPDDF
                     SILEAVFP"
     gene            16981..17424
                     /locus_tag="P5164_01535"
     CDS             16981..17424
                     /locus_tag="P5164_01535"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY42602.1"
                     /translation="MEERRFLADVPVVGEAVAWARAEADAAGLPDARGLDLELAVEEA
                     LMNVCTHAYGGRGGEMTVRAGRTDEGFVVEIADSGPAFDPLSLPSPDTAAPLDERRIG
                     GLGVLLVRRVTDGVRWRREAERNVLTLVFSFPPPSGAETSPAGGA"
     gene            17499..18815
                     /locus_tag="P5164_01540"
     CDS             17499..18815
                     /locus_tag="P5164_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42603.1"
                     /translation="MYVTLGELRVPAKASLVPLVASFASRGAAALGLDEKDSDALGIA
                     AEEVFSHLAACAAPGRDVVLRLDDGGWRAGLEVRFAAEPGRVDFRPFNLTFRTTPEED
                     PLLARTALAIASRMVDRFQLRRQDREILLTLTRERRFPKGEPPPPEPRPEGAVSARAA
                     RPHEIPLAAGLAAARHPAGQVLPAFGSPGTVSAMAAAGDLDAVVAADAAGRLAGALFF
                     RPEGDRLVELLGPFLFQEPAVEGAARLLVDACLAALGRAGRTGVLARNPTDAMPPNAL
                     EPLGPLDLRGADGVVRRVHARFRQLDEDPGGIAHLHPRLEPFVREALRRLAFARTFRL
                     LEGPAVPPGEGTAFATEIDRRNGQAHLKPLWLGADARATLDGLLAALSAEGLANPTAE
                     LDLGEPWHAGFAPALLDAGFEPRLVVPWAATGDVLLLQRTTEPSAR"
     gene            18812..19918
                     /gene="hisC"
                     /locus_tag="P5164_01545"
     CDS             18812..19918
                     /gene="hisC"
                     /locus_tag="P5164_01545"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRY42604.1"
                     /translation="MTAWTLEPLVPEFVRQFEAYVPSRPDPELMALYGVPRLFRLNNN
                     ENPLGPPAAAQEVLRRFPPPRAAVYPSGDSFYLRRRLGAALGVDPEQVLVGNGANEAI
                     GFVIKAFCQEGDNIVTADRTFAVYEWTARFSGIDARLVPLSDLGFDDEGMLARIDART
                     KVLFVCNPNNPTGTFWDRERLRRFVERVDGRAVVVVDEAYAEFVEDASYPDGLALLPD
                     HPNVVVFRTFSKMWGLAGLRVGYLVGERKVVDTVRRTCTVYSVNGLAQEAALAALGDD
                     GHVPATRLLVREEKAYLASELSAMGLTVNAREGPYVMVELPVSDTLAYRRLMAEGVMV
                     RTMTGFRFPGWIRVSIGRHDAMEAFVGALRRALA"
     gene            19915..>21444
                     /locus_tag="P5164_01550"
     CDS             19915..>21444
                     /locus_tag="P5164_01550"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein"
                     /protein_id="HRY42605.1"
                     /translation="MRVGEPARIGVPLPRADAGPKAAGEPLYAGDTVPDGALWAGVRR
                     AGVPRARLVSIDVGRAAALEGVVAALTHRDVAGTNRQGVIRRDQPVLVDGEVRHAGDP
                     LALVVAESPEALERALALVDAVLEPLPPLLDAAEALRDGAPVLHPDHPGGNVLLEGRL
                     VSGSGEAAETGCDSVVEVLFETPRQEHACLETAAGWAVATPDGAVEVTCSTQTPWRDR
                     AETAEALGLDPARLRIVAPYLGGGFGGKDGLTVQSLLALAALHAGHRPVRMRWGREES
                     IVAGVKRHAGRMTVKLGATADGTLRFASADVLLDTGPYDHLGGAVLELALEHATGPYR
                     VPNAVVTGRAVYTDNPIGGAFRGFGVTQVTAAVEQAVDLLADRLGLDRLEIRRNNALS
                     RGDVTGTGFTLLCSVGLGDALDALARHPLFTGREAWKAEAGPFRRRGAGIAALWHGAG
                     YGPVVPDVANAKVELLPSGRFRVFCGVADMGQGNVATNAQVAGAVLCQPPEAVELVLP
                     DT"
BASE COUNT         2479 a         8452 c         7637 g         2876 t
ORIGIN      
        1 gtccagctct tcccgccgtc cgtcgtccgg tagaggcccc gctcgccgcc gtcggtgtag
       61 agcgggccgg tcgcggcggc gaagacgacg tccgggtcgg cggcgtcgac gaggatcttc
      121 ccgatccggt gcgcgtcgtg aaggccggtg ttcgtccagc tcttcccgcc gtcgatcgac
      181 ttgaagacgc cggtcccgga gtaggtcgtc cggctcgagt tcgcctcgcc cgtgccggcc
      241 cagaggacgt tcccctccgc gtcgcccacc gcgatcgcgc cgagggcgct cgccgactcg
      301 cggtcgaaga gcggcgtcca gctccccccc ttgttgtcgg tcctccagag gcctcccgtc
      361 gcgaaggcga cgaagagcgt gccgggtcgg ctccgggggg cgtcgaggtc gacgatccgc
      421 cccccctgca gctcggggcc gacgttccgg aacgagaggc cggcgaacgg gctcgccttc
      481 tccatcgcga gccgcttctc gtacccggcg aggcggggtg cggcgggcat cccctgagcc
      541 ggggggtgga agcgcttcgc ctcctcggcc ttcgtggccg ggggggccga gaggaggggc
      601 gcggcgacga ggagggcgga ggcggcgagc cagagggggt tcttcatgtc cgtctcgcac
      661 gagatcgtac agaagagccg cctcccctcc ggcgtgcgcc gttcccgggg atccggggcc
      721 ccgcgcccgc accggcggcg tcagggcgtc ggagggggcc ctccggtccc cgggcccgcc
      781 tcgacggtga accgggccac ctcgtcccgc aggagcgcga cggcgcgccc cagctcctcc
      841 tcggcgtggc ggaagccctg gatctcctgg gccgcctgtc gcgccgccac ggcgagcgcg
      901 ccgacgccgt cggtgatctg gccgacgccc tccgcctggg cggagacgtc ggcggtgacg
      961 gacgcgatct tcgggtggag gctctccgcc tcctcgagcg ccctttccat ctgccgcgtc
     1021 agccgcccga gcccggcgct cccgcgggcc atctcggcgg agagctggtc catctcgacg
     1081 agcccctcgg cgaccgcccc ctgcatctcg gcgacgttct tctcgatgtc gagggtcgcg
     1141 atcgccgtct ggtcggcgag acggcggatc tcctgcgcca ccaccgcgaa acccgcccct
     1201 gcggcgcccg ccttctcggc ctcgatcgcg gcattgaggg aaaggaggtt cgtccggtcc
     1261 gcgacggcca cgatcgtcga caccatcgag gtgatcttct ccgtcctctc gcggatcgcg
     1321 ccgagccgca cggagacggt ggccgtgctc ctcgagagct gcgcgaggcc cgcctccatc
     1381 gccgacaggt ccttccgtcc ctcgtccgcg agacgggcga cgcccgcggc gacgccgcgg
     1441 actccctgaa cggccccgga aacctcctcc cccgtcgcgg cgacctggcg cagcgccgtg
     1501 gcgatctcgg tcgtggaggc gctgaagtcc tggacggccc cctcctggcg gtgggcgctc
     1561 gcccgcatcc ggtccgccgt cgcagccagg cggtccgtcg cctggcccac cttccccacg
     1621 aggctgccga gacgggtcgt catctcgccg atcgcccgca ggagccgccc cggctcgtcg
     1681 ggcgcggagt cctcgatccg gaccgtcagg tcgccgtccg cgacgctccg cgccacgaca
     1741 acggcgcgcc cgaggcgcgt cgcgagagag cgggcgagaa agacgatcag gaagacgacc
     1801 gccgcgacgc cgaggccggt gaacaggagc gtccgccgga tcgtccggtc gatgggggcg
     1861 aggacctcct tctcggagac ggtcatgacg agcgtccagc ctcccgtcgg gacgcgagcg
     1921 ctcgcgagga aggcccggcg gccgtcgaag gggtcggtga cctcgcggac gaccggctcc
     1981 cccgggccgg ccttcgcccc ggaaaggagc tcggcgaggt cggtgcgggc accggccgga
     2041 acgagcgcgg ccaggcgccc ctcgtccagc tcgcgcattc ctcccttctc gacgtacgcc
     2101 ccggcgacga cctctccgtt gccgccgagc ctcaggtcgg cggcgcgcat cgtccggacg
     2161 tcctgctcga gcgtcgcggc cacgacgcgg cccgcggagc tgaggaggta gaagcgcgcc
     2221 gtctcgaagg gccgcagctc cctcagccgg gcgtcgaggt cgtcgagcgc ccggtcgacc
     2281 ccggtgatcc cggcgaagcg cccgccgacc atgatcgcgc tcgtctgctc caccataagg
     2341 acgcggtccg aatagcggta cggctcggag acgagggtcc gctcgcccga tcccccggcc
     2401 accctgcggc gaagagcgtc gtagtagaga gccgtctcca tctcggcggc cggggtgcgc
     2461 tgctccacga tccggtccgg ctcgccggga tccctgctgt acgcggcgga gaagcggccc
     2521 gtcccgtcga tccagaagac ccggcccccg agctcggcga ggtcccgcgc gtcgcgcccg
     2581 tcggcgttcg cctcgtagag gacgtaggcg gtgacgaagt cccggcgctc ctcgagcacg
     2641 cgccggcaga gcgccgccga ctccgcgcgt cggccgaaca ggcccgcctc ctgcgcgagg
     2701 ctcatcgtcc gcgcgacgag gaccgcctcc cggctctccg cgtcgatcgc cgcggcggcg
     2761 tccttcagag cgcgcgccat cgaggcgcgc gtcgcccgga gcaggagcgt gcggcgctcg
     2821 cggaggtcgg tgaaagccag gagcgcctgg atcgcgacca acgggaggac ggcatacagg
     2881 aggaacttcg tcctgagcgt cacacggccc tccggcgcaa gcgcatggga actcctcccg
     2941 gctcgagatt agcgcggcgg gcggggccct tttccgctcc ctcgggcggc gccctccggt
     3001 ctccggcgat ccgtcggcgg cccgccggcg gcgggcgtgt acgattccga ccgcttcctg
     3061 gaggaggacg gagatggcgc tcagcatcga catccagcga ccgggcgacg cgctctggac
     3121 ggcccgggtg accctcgccg gcagcctcga cgggacgacc gccccgcgcc tcgaggaggc
     3181 gctgaagccg ctcctcggcg cggtgcggca cgtcgtcttc gacctcgccg acctgaagtt
     3241 catctcgagc gccggcatcc gcgtcctcct cgcggcgcgc aaggagctga aggcccggga
     3301 cggctcgttc gcgatgcacc gcctgcagcc gcagatcgtg aaggtcttcg agatcatcgg
     3361 cgcgctcccc ggcttcgaca tcttcacgag cgagaaggag ctcgacgact acctggccgc
     3421 gatgcagcgg aagcacgcct ccccgggcgt gctctgagcc gcccctcgag gcggtccgcg
     3481 tcccgtcgcg gcgtacaatg gagggccggc ccgggtcctc gttcgaaggg tccatcggcg
     3541 cgggggaggg gggcttgagg cgtttcggcc tgcggacgaa gctcgtcgcg ctcgcgctcg
     3601 ccgctgcggc ccttccggtc ctcgccttcg tgaccctcac ggccgccgga aggcgctcgg
     3661 cctcccggga gatcgccgtc gagctcgacg cggtggcccg gatgaacctc ggccagtacg
     3721 tccgggacct ctacgccgcc tgcggcaccg tcgacgccct cctcgcccct cgcctctggc
     3781 aggggcagtc gcagctccag ctcgcgctcc ggaacgccgg cggcctctcg tgggacggcc
     3841 ctctcgtccg ctgggaggtc ccgggggcgc cgggcggcct cgtggcggcc ccccaggtcc
     3901 gcgtgggcgg ccgccccctt tcgagggagc gctcgttcca ggtcccggag cccgccgtcg
     3961 acgctctcgc ccgggactcc gccatcctcg cgatcctcta ccagaggctc gacgagcgcg
     4021 gcgacatgct cgccgtcgcg agcagcgtgc ccgcggagga cggctcgcgg ctgaccggca
     4081 gcctcctccc cgccgccacc gccgacggga gcccggagcc gtcggtggcc cgggctctcg
     4141 ccggatcggg ccaccgcgcg atggcacggc tcggcggccg gatgtacctc acctccaccg
     4201 agccgatccg cgacgagtcg ggacgggtca cgggtatggc gctcgccgcg gtcggcggcc
     4261 gcgagctcga cggtctctac cggtcgatcg tcgagacgaa ggtgggcgag cgcggctatt
     4321 tcgcgatcct cggagcgaag ggccggcagc gcggggtcta cttcgtctcg aaggccggca
     4381 gccgcgacgg agaggacatc tgggacctgc gcgacgccga gggccgcccc ttcgtgaagg
     4441 agatgatcga cgaggcgctc cggagggagc cggggacgat ccggcacaac cggtacgcct
     4501 ggaggaaccc cgacgacccg gaggcctgga cgcgcgtcgt cgccgacacc tacttcaagc
     4561 cctgggactg gtacgtcaac gccgggacga acgagtcgga cttcctcggg gcgagcctgc
     4621 ggaccgaagc ggccttcgac gccatcctcc gccgcgccgc cctcggcgga gcgatcgcgt
     4681 tcgctctggc cgcttcgatc gcgctcctcc tcgcccgacg cctcgcccgc cccctggcgg
     4741 cgctcctcga ggagacggcg cggctcggcc gcggcgacct tcgcggcgcg acggtcccgc
     4801 ccccgctgcc ggcccgctcc cgcgacgaga cgcggctcct ctcctacgcc ttcggctcga
     4861 tggcggccgc gttcgcctcc ctcgtccgcc aggtgcagcg ctccggaatc caggtcgtcg
     4921 cctcggccac ccgcatcgac gcctcggccc gccagctcga ggactccgtc gcccaccagg
     4981 ccgccacgac gccccaggtc tcggcctccg cccggcagat ctccgccacc tcccggcacc
     5041 tcgccgagac ggtcgacgcc gtcgcttccg tcgccgcggc caccgccgcc cgcgccgcgg
     5101 acggccgcag cgccctctct cagatggacg gctccgtccg cctcatctcg agctcgtcgg
     5161 cctcggtctc ccggaggctc tcggcgatct ccgagcgagc gcgctcgatc tcctcgctcg
     5221 tcaccaccat ctcccgcgtc gccgagcgaa cgaacctcct ttccctgaac gcctcgatcg
     5281 aggcggagaa ggcgggcgag caggggcgcg ggttcgccgt cgtcgcgcgc gagatccggc
     5341 gcctcgcgga ccagaccgcc gccgcgacgg tcgacatcga gcggaccgtc cgcgacgtcc
     5401 tcgcctccgt cgcgtccggc gtcctggaga tggacaagtt cgtcgacgag gtgcgcgtcg
     5461 ccaccgccga cgtcgagcgc ctcggcgcgc tgctggcctc gatcgtcgag gacgtcgccg
     5521 gactgacgcc ccgcatcgac tcggtgagct ccggaacgcg ggagcaggcg gaaggggccg
     5581 cgcagatctc ggaggcgatg tcgctgctcg accgcaccgc cgccgcctcc cgggacaccc
     5641 tggccgagtt tcgcggagcg gcggagtccc tcggggtcgc ggtcgcctcg ctccgggccg
     5701 aggtggaaag gctccgggtc gacccgtgag cgccgccccg catcctccgg accgcgcagg
     5761 tgcgcgcgaa gcggggacgg cccgtccggg tgccgcggcg tcgggcgctc gcatgaggac
     5821 gaggatcctc gggctcgccg cgctcgccgc cgcgatcccg ctgccgttcc tctggctcgt
     5881 gacggccgcc ggggcccggc aggcctcccg ggagtcggcg gccgagctcg aggagctcgg
     5941 ccgccagaac gtccggcagg tggccggcga cgtcgcgcgc ctctgcgcca gcgtgaacgg
     6001 cctgatgcag ccccggctcg accgcggcgt cgccgccgca cgggagatcc tcgcccggca
     6061 gggcggcctc gagcccgacg ggacccggcg tacctggtcg gcggtcgacc aggagagtcg
     6121 ctcgatctcg ccggtcacgc tgcccgggct cgccctcgga ggggcgccgc tcgtcgagaa
     6181 ccgcgacccg aaggtccgct cgccgctcgt cgacgagatc cgggagacga ccgggatgtg
     6241 gagctccctc ttccagagga tgaacgagaa aggggacatg ctccgcgtcg tcagcagcat
     6301 cctgacgacc gacggtcggc gcgggaccgg cctctacctg ccggccgtcc tccccgacgg
     6361 cgcgctgaac cccgtcctcg cgtcggtcct cgccggccgg acgtaccggg gcacgtctct
     6421 cgtcgccggg aggatgtacc tcgccgcgta cgaccccgtc cgggaccgct ccggccgcgt
     6481 cgtcggcatg gtcgccgtcg cgttcgactc gcagatcctc gaggggatcc ggcggtcgat
     6541 cgcgggcacc gtcgtcgggc gccgcggccg ggtcgccgtc ctcggcacgc gcggcgcgag
     6601 ccggggcgtc tgggtcgtct cgcgcgagag ggctcgggac ggacagagcg cctgggagga
     6661 gcgcgacgcc gaggggaggc cggtggtgca ggagctcgtc gcgctcgccg gctccctccg
     6721 gcccggcgag gtcgccttcc accgctcgtc ctgggccggg tcggacgacg ccggcgcctt
     6781 tcggaagctc tccggcgtcg cctacttcga gccctgggac tggctgatcg aggccggaac
     6841 ggacgaggcc gacttcctcg gcgcgacggc tcggaccgac gcggccttcg atcgcatcct
     6901 gcgccgggcc gcgctcggcg ccgccgccgc gttcctcctc gcggccggga tcgcgctgct
     6961 cctcgcccac cgcctctccc gtccgctcgt ccgcctcctg gccgagacgg agcggctcgg
     7021 tcgcggcgac ctgcggggag ccggagctcc ctcgaccggg tcctcccgtt cccgcgacga
     7081 gaccggcctc ctcgcgcacg ccttcggctc catggcggcg acgctggccg cgctcgtccg
     7141 ccaggtgcag cgtgccggga tccaggtcgt cgcgtccagc acccgcatcg ccacctcggc
     7201 ccgccggctc gaggactccg tcgcccacca ggccgccacg acgcccgagg tgtcggcgtc
     7261 cgcgcgccag atctccgcca ccgcccggca tctcgccgag acggccgacg ccgtcgcctc
     7321 cgtggccgcg gccacctcgg cccgtgccgc cgacgggcgc gccgccctct cccagatgga
     7381 cgactcggtc cggcgcatcg aaggctcctc ggcctccgtc tcccggcggc tcgccgacat
     7441 ctccgaccgg acccggtcga tctcctcgct cgtcacggcg atctcccgcg tcgccgatca
     7501 gacgaacctc ctttcgctga acgcctccat cgaggccgag aaggcgggcg accagggcga
     7561 ggggttcgcg gtcgtcgccc gcgaggtccg gcgcctcgcc gaccagacgg ccgtcgccac
     7621 gctcgacatc gaacgcacgg tccgcgacgt ccagtccgcc gtctcctccg gcgtggccga
     7681 gatggaccgc ttcgtcgagg aggtccgtct cgccggcgcc gacgtcggga ggatctccgc
     7741 cctcctctcc tcgatcgccg aggacgtcgc cgacctgacg ccccgcctcg agtcggtccg
     7801 ctccgccgcc cgcgagcagg cggaaggagc cgggcagatc tccgaggcga tgctcctcct
     7861 cgaccgctcc gcctcctcct cgcgcgagac gctcgacgag ttccgcgccg ccacccgctc
     7921 actccaggac gccgtcgcct cgctccgggc cgaggtgggg cgccttcagg tcgacccatg
     7981 aaagaccgac tctcccgact tccgatcgcc caccgcctcc tcctctccag cctggcattc
     8041 ggggtcccga tcgcggcgct cgtcgcgatc ggcttcctcg cggtgcagca gcgcctcgac
     8101 accgcccgcc gggagaagct cggcgtgagc ctcgcccagc cgctctgcga gctctccggc
     8161 cacgccgccc gccacggccg gctcgcgtct cgggccgccg ccggcgaggc ggtcggcggc
     8221 gaggctcgag agcaggccgc ccgggccgtc gacgccgcct tcgaggcgct gcgcgccccg
     8281 ggggcgcgcg ccgccgccgg acggctcggc ctggctcccg aggggcggcc cgacccgctc
     8341 gaggagctgc tcgccgcctg gcgccgggtc ccttcagccg gctctggcga cgcacggagc
     8401 gtggagcgcg cccaccgcac ggtcctcgac ggcaccgccg ccctcctcct gcggacgctg
     8461 acggagtccc ggctcgcctt cgacccggcg ctcgactccc acttcctggc cgaagccgcg
     8521 ttccgagccc tcccggccgc atccgcccgg atcgcggcga tgctcgccgg cccctcgccg
     8581 tccgccgggg agcgggccgc gctcgccttc caggccgacg agctcggccg cgccgagctg
     8641 ccccggctgg ccgaggcgct cgcggcggcc ctgcacgacg acgcggggcc tcgtcggtcg
     8701 agcgggagcg acccgaccgg gcttcccgcc ctccgggacc ggttcgacgc ctcggcgcgg
     8761 gagctccttt ccgctgcgcg ccgggccgcg gccgaggcct cggaggcggg gtccgtcgac
     8821 ccttccgcgg acgcggccct cacctccgcg ttcgacctcc ggcgcggcgt cgccgccgag
     8881 ctcgaccgcc gcctggacgc gcgcgtgacg cgctacgagc gggagcggct cgccgtcctc
     8941 ctgctcgcgc tcctcgccgt cgccatctcg gcgaggcacg tcggatacat cgtcttcggc
     9001 atctcccggc ccctcgccag cgtcgcggac gccgcccatc tcgtggcggc cggcgacgtc
     9061 gccggcgcgc gggagcgcgc ttcccgtgtg ccggccggcg acggcccgag ccggaacgag
     9121 atctcgcgcc tcggcggggc ggtccgcgag atggccgacg gcctctcctc actcctgctc
     9181 gaggtgcagg ccgccggcgg ccacgtgaaa gagacctccg cgcggatcgc cgcgaccgcc
     9241 cgggagctgg aggcgacgtc ctcggagcag gccgcctccg cgaacgaggt gagcgcgacc
     9301 tcgcgcgaga tccaccggcg cgccgagggc ttcgccgcga cgatcggcga ggtcgggaag
     9361 gtcgtccgcg aggcgtccga cctcgcgggc gacggccggc gcgggctgga ccggatggag
     9421 tcgagcatgg accagctgac cgccgccacc gcctcggtct ccgagcggct cgcggcgatc
     9481 agcggaaagg ccgaggggat cggcgcgatc gtcacgacga tcgggcgggt cgccgagcag
     9541 acgaacctcc tctccttcaa cgcggcgatc gaggcggaga aggccggaga ggccggcaag
     9601 ggcttcgtcg tcgtcgcccg cgagatccgc cggctcgccg accggacggc ggcagcggcc
     9661 gtggagatcg gcgaggtcgt cgagagcatg cggtccgtcg tctcctccgg cgtgatggag
     9721 atggaccgct tcgtcgacca ggtgaggcgc gaaggggagg aggtgcgccg catcggcggg
     9781 cagctcggga ccgtcatcga gcaggtggag gctctgaccc cccgcttcgc cgaggtcctc
     9841 gccgccgccg aggcgcaggc gggctcggcc cgcgagatcc gcgaggccgt ctcccagctc
     9901 tctgcggccg ccggacagac gcgggacacg ctggaggagt ggagccgggt ggcgcagggc
     9961 ctcgacggcg ccgcgcgctc gctcgccgcg caggtggaac ggttccgcct cgccggggac
    10021 ggcgccgcga cgaaggcgga cgggggctga gcggtgctct acgtcctcct ctcggccggg
    10081 ccccgcaggt acgcgctggc ggcccgttcc gtcgtcgagg tcgtgccgct ccttcgcctg
    10141 gagccgctcc cgggcgcccc gccttcggtt cgagggctct tctcgtaccg cggcacgccg
    10201 gtgccggcgg tcgatgtctc cctgctcgcg ggggccggag cggttccgga gaggatgagc
    10261 acgcgcatcg tcgtcgtctc ggtcgaggag cccggaggaa ggcgtcggct cgcggggctc
    10321 ctggcgacgt cggtcaccga gaccgtcacg cgctcggcgt ccgacgcccc gcccgcgggc
    10381 ctgcgccttc ccgaggcgcc ctggatcgag tcggcgttcg tcgtcggcga cgaggtcgtc
    10441 ccgtgcgtcc gcgtcgaggc cttcctcccc gccgaggtgc ggtcgctcgt cgacgcggcg
    10501 gagggggcgg cgtgacccgc tcggtcgacg cggtcgagtc ggtcctcgcc gcccgcctcg
    10561 ggctcgacgc ggagtccctc tcccccggcg ccgtcgagcg cgcggtggcg cggcggacgc
    10621 aggcgctcga cctcgacggc ccgtgccgct acctcgcccg cctcctcgag gacggaaccg
    10681 aatggcccgc gctcgtggac gaggtcgtcg ttcccgagac ctggctcttc cgcgacgggg
    10741 ggccgttcga ggagctggcc gcgcacgtcc ggtcgggccg cgccccctcc gccgacgagc
    10801 cctaccgcgt cctctcgatg ccctgcgcga gcggggagga ggcctggtcc gccgcagccg
    10861 tcctcgccga ggccgggctg tcgccccgcg aggcgctcgt cgacgccgtc gacgtcagcc
    10921 ggaggctcgt cgagacggcg cggcgcggcg tctacggcgc cccttccttc cggtcccccg
    10981 aggccgaggt gcgcctcggg gcccggcgtc tcgaggacgg acggcgggag gtggacgcgt
    11041 cgctccggcc ctacgtccgc ttccacgtcg agaacgccct cgagttcgaa gccgggcttc
    11101 cccttccctg ctacgacgcg gtcttctgcc gcaaccttct catctacctc acgcccgagg
    11161 cgcgccggcg cctgacgctc cgcgccgacc ggctgcttcg ccccggaggg ctcatggtcc
    11221 tggggcacgc cgaggcgttc acgaccttct tccccggcta cgtcccctcc cggaggcccc
    11281 gggcgttcgc ggcgcagaag cctgctgacg gagtggagcg cggccgcgtc cccgccgtcg
    11341 tcccgaggga gtcccgcggc gggtcgacgc cccgggcgtc gacccgctcc gtcggcccga
    11401 ggacgccggc cgcccccggg acggtcccgc tctcgtcgtc tcccgaggcc ggagcggcgc
    11461 tcctcgtcga ggcgcggcgc ctcgcgaacg ccggcgagcg cgcgtcggtc gagcgactct
    11521 gcgtccgggc cctcgcggcc gaccccgcct cggtcgacgc gcgcctcctc ctcgccgaga
    11581 cgacgctggc cgcgggcgac tctccccgcg cggaggccga gctggagcgt gtcctctatc
    11641 tcgccccccg gaacgagacc gccctcctcc tcctcgcccg cctgcgggac cgggcggggg
    11701 accgtgaggc cgccgcccgc ctgcgcggcc gggcgcggag ggccgcgagg gccgcttcgt
    11761 gaacgcacgt cttctcgctc ccgacgactg ctggagccgc atcggcgtct ccggcgacgg
    11821 ctcctgcgag aagctccccg agctcgtcca ctgccgcaac tgcccggttt tctcggccgg
    11881 cggccggacg ctcttcgaac gcgagccgcc tcccggctac gccgaggagt ggacgaaggt
    11941 gctcgcgctc ccgaagcccg tcgaggcccc cggcacgctc acggcgaccg tcttccgggt
    12001 gggcgaggag tggctcgccc tcgaggtctc gcacgtcgtg agggtgggcg tggcgcgtcc
    12061 gatccggcgc gtcccgcacc gctcgggaga ggtgctcctc ggcctcgtca acctcgacgg
    12121 cgagctggtc ctctgcgcct cgctcgcgtc gctgctcgga gtgtccgacg tccccggcgc
    12181 cccgccgccg ggccggtacc tggccgtcgc cctcgccggg gagcgctggg tctttcccgt
    12241 cgacgagatc gacggggtcc accggctgga gcaggagggc ctcggcccgc tgccgtcgac
    12301 tctctccgag gcgcgggcgc ggcacgcgcg cggcctcgcg cggacgtccg agcggaccgt
    12361 caccctgctc gacgccgcga ggctcctcgc ggggctccgc gagagagggg gcctgtcgtg
    12421 agcggcctcg acgaggggct cctcgacctc ttccggacgg aggccgaggc gaacgcgctc
    12481 gcgctggagt cgggcctcgt cgcgctcgac accgggaccg cgacgaagga ggtcctcgag
    12541 ccgctcatgc gggccgccca ctcgctgaag ggggcggccc ggatcgtcgg cctcgacgcc
    12601 gccgtcgtgc tcgcccacgc gatggaggac gtcttcgtgg cagcgcagcg cgggaagctc
    12661 gccctcgtcc ccgcgcacgt ggaccgcctc ctcgccgggg tcgacgtctt cgcccggctc
    12721 gcctccctcg acccgtccgc gatcccggcc cggctcggcg cgatggacgc cgagatccga
    12781 acgctcgtga cggagctcgt cgccttctcc tccgccgggg cgcccgcggc cgctccggca
    12841 ccgaaggtcg atcccgagac cccggacgcg gcgcctcccc cggcggcggc ctcgccggcg
    12901 gtcgcgcccc cgactcccct ctcgccgacg tcccccgtcc ccgctccggc tccgccgccc
    12961 gcgccctcgg gccccggcga gccggcggcg tcccgcgagg ggagcgtcgt cgtcgggacg
    13021 gggaccctcg accgcctcac cggcctcgcg ggcgagagcc tcgtccaggc ccggcgcgcc
    13081 cccgacctga tgcgcgagtt cgcgaccgtg aaggcctcgc tcgcgaggat gtcgggcctc
    13141 ctggaggacc gccccggcgg ccggcccggc gagaggatcg tcccggtcgc cgcgctcgcc
    13201 cgcgaggtcg aggagtcgcg ggccgcgctc gtccgcgcgc tcgacgccct cgagaggcac
    13261 gcgcatcgga ccgaggagat cgccgaccgc ctccacggcg aggccgtctc cgctcggatg
    13321 cgcccgttcg gcgagggggc ccgcggcttc ccgcgcctcg tgcgcgacac ggcgcgcgag
    13381 ctcggcaagg agatcgtcct cgaggtccgc ggcgaggaga cgcgggtgga ccgcgacatc
    13441 ctcgccctcc tcgaggcccc gctcgggcac gccctgcgga acgcctgcga ccacggcatc
    13501 gagctgcctg acgagcgcct cgtgaagggg aagccccgcg cgggccggat cgtcctgacc
    13561 gccgagcacc gctcgggcgc cctcttcgtg acggtcgccg acgacgggcg cgggctcgac
    13621 ccggagcgca tccgggcccg cgtcgccgag cgagggctcg ccccgcagag cctcctcgcc
    13681 ggcatcaagg aggccgagct cttcgagttc ctcttcctgc ccggcttctc cacgacctcg
    13741 agggtgacgc agctgtccgg acgcggcgtc ggcctcgatg tcgtccagag catggcccgc
    13801 gacgtcggcg gacgggtcgg cctgacctcg accctcggcg ccggcacggc gctcacgctc
    13861 cagctcccgc tcacgctctc cgtcgtccgt tccctcctgt tcctcgtctc gggcgagccg
    13921 ttcgccctgc cgctctcgcg gatcgaccgc gtcctggagg ttccggcgga ggagctccgt
    13981 acggccgagg ggaaggcctt cgtcgacgtg gacggacggg ccgtcggcgt cctcttcgcc
    14041 cggcagccgc tcggcctgcc tcccgtcggc gagccgggcg gcaccgtgaa ggtcgtcgtc
    14101 gtcagcgacc gcctcgatac ctacgggctc gtcgtcgact ccttcctcgg cgagcgccag
    14161 ctcgtcctgc gcccgctcga cccgcgcctc ggcaaggtgc ccgacctctc cgccgcggcg
    14221 ctcctcgagg acggcacgcc ggtcctcgtt ctcgacgccg aggacctcgt ccgctcgatc
    14281 cacgggatcc tctccgggtc ggaggtcgag tcggtccggc cgaaggcggg ctccgggccg
    14341 gagcgaaggg tccgacggat cctcgtcgtc gacgactcga tcaccgtccg ggaggtcgag
    14401 cggcggctcc tcgcgagcca cggctacgag gtggacgtcg ccgtcgacgg gatggacggc
    14461 ttcaaccgcg tccggtcggg gcgctacgac ctcgtcgtca ccgacgtcga catgccgagg
    14521 atgaacggca tcgagatggt gacgcgcatc cgctccgagt cctcgctcgc acggctccct
    14581 gtggtgatcg tctcctacaa ggaccgcgag gaggaccgcc ttcagggcct ggcggccggg
    14641 gcgagctact acctgacgaa agggagcttc gatgaccgga gcctcctgac cgccatcgcc
    14701 gacctgatcg gagagccgtg aagatccggg tgggaatcgc cgtcgggccc gggcccgtcc
    14761 aggaggcgct ccgcgaggtc gtcgaggagg acccgggccg ggagctcgcc tgggccgctc
    14821 cagggccgtc cgaggccgcc tcctggtgcg cgcacgacac ccccgatctc ctcctgctgg
    14881 gcctcgacga gaggagcgtc gaggcgacgc gccggatcct cgcggtctcc cccacgacga
    14941 tcctgatcgt cgccgccgcg gattcggccg acaccggccg cgtcttcgac gtcctcgcgt
    15001 ccggcgcgcg ggacgtcgtc gagccgcccc gccggaggcc gggcggcctc ctcgagaacg
    15061 aggcccccgt tcgcgccaag ctcgccctgc tggcccgcct ggccgcaccg gccgcctccg
    15121 ccctgcctgc gcggaccggc cgcgccgagg cgccgcccgc cttcccgccg ctcgtcgccg
    15181 tgggcgcgtc caccggcggg cccggcgcgg tcgggagcct cctcgcggcg ctgcccgcgg
    15241 acctcggagc gccgatcgtc gtcgtccagc acctcgacga cccgttcgtg aagggactcg
    15301 ccggctggct cgcctcgcgc tccggacgaa gcgtgcgcac cgccgtcggc ggcgagcggc
    15361 cccggccggg ggacgtcctc ctggcgggca cctcggcgca cctcgtcgtc gctgagaacg
    15421 gcactctcgc ctacacgccc gagccgcggg agtgccccta ccgcccgtcg gtcgacgcgt
    15481 tcttctcgag cgccgcccgg agctggcccg gaaagggcgt cgccgtcctc ctgacgggga
    15541 tgggccggga cggcgcggaa gggctcctcg cctgccggtc ggccggctgg ctcacgatcg
    15601 cccaggacga gaagacgagc gtcctctacg ggatgccgcg cgccgcccgc gagatcggcg
    15661 cggcctcgga ggtcctgccc cttcccgaca tcgcgcccgc cgtggcgcgc cgggtccgcg
    15721 aggagcggag ccggaccaga gagagcggag gaatcggatg agcaacgagc ctggcaacgc
    15781 cctcgcgacg gagaccggcc ccgtctcccc ctcgagccgg ccggtgaccg tcctgctcgt
    15841 cgacgaccag gcgatcgtcg gcgccgccgt gcggcggatg ctccagcccg aggagggcat
    15901 cgtcttccac cactgctccg acccgacgaa ggcgatcgag acggcggagt cggtggagcc
    15961 gacggtcatc ctccaggacc tcgtcatgcc gggcgtcgac ggactcgacc tcgtgacgaa
    16021 gtaccgcgag acggcgtcga cccgggacgt gccgatcgtc gtcctctcca cgcgcgagga
    16081 gcccgagacg aaggccgagg ccttcgcccg cggcgcgaac gactacatcg tcaagctgcc
    16141 ggaccgcctc gagctcgtcg cccgcgtccg ctaccactcg aaggggtacg tggcgctcct
    16201 cgagcggaac gaggcgacgc ggcagctgat ggccgagctc gccgaggcgt cgcagtacgt
    16261 gaagagcctg ctgccccccc ctctcgagga gccgcgcgtg aggatcgact ggcgcttcct
    16321 gacgagcacc tctctgggcg gcgacgcctt cgggtacggc tggatcgacg acgaccacct
    16381 ccagctctac ctcctcgacg tctgcggcca cggcgtcggc gcagccctcc tctccgtcac
    16441 cgtgatgaac gtgatccgct cgcaggccct ccccggcgcc gacttccgga agcccgacga
    16501 ggtcctctcg gcgctgaacg aggcgttccc gatggcgaag cagaacggct cgtacttcac
    16561 gatctggtac ggcgtctacc gcccgtcgac gcggaccctc gtccacgcga gcggaggcca
    16621 ccccgccgcg ctcctcctcg aggggccggc cgggacggct ccggccgcgc gcctgagggc
    16681 cccgaacctg atcatcggct tcgtgccggg gaagacgttc cggagccagg aggtggcggt
    16741 ccctccgggg gcgcggctct tcctctacag cgacggcgtc tacgaggtgg agatggccga
    16801 cgggcagatc tggccgttcg acgacttcgc cgcgttcatg gaggcgcctc ccgcgccggg
    16861 cgcttcccgc atggacgagc tcctcgccca ggcccgccgc ctgaaccggt cggacgccct
    16921 tcccgacgac ttctcgatcc tcgaggccgt cttcccgtga cgggcgggcc gggtgaggcg
    16981 atggaggagc gacgcttcct cgcggacgtc ccggtcgtcg gcgaggccgt cgcctgggcg
    17041 agggccgagg ccgacgcggc ggggctgccc gacgcacgcg ggctcgacct cgagctggcc
    17101 gtcgaggagg cgctcatgaa cgtctgcacc catgcctacg ggggacgcgg gggcgagatg
    17161 acggtccggg ccgggcggac ggacgaaggg ttcgtcgtcg agatcgccga ctcgggcccc
    17221 gcgttcgacc ccctctcgct cccgtcgccc gataccgcgg cgcccctcga cgaaaggagg
    17281 atcggcggcc tcggcgtcct gctcgtccgc cgcgtcaccg acggcgtccg ctggcggcgc
    17341 gaggcggagc gcaacgtcct gacgctcgtc ttctccttcc cgcctccgtc cggagcggag
    17401 acctcgcccg ccggcggcgc ctgacggccg gcgggcgagg agctccgccg cccgttccgc
    17461 ccgcgatcct cccgtcctcc gccgatagaa tcctccggat gtacgtcacg ctcggtgagc
    17521 tccgggtccc cgcgaaggcc tcgctcgtcc ctctcgtcgc ctccttcgcg agccgggggg
    17581 cggcggctct cggcctcgac gagaaggatt ccgacgcgct cgggatcgcc gcggaggagg
    17641 tcttctcgca cctcgccgcg tgcgccgctc cgggacggga cgtcgtcctg cggctcgacg
    17701 acggcggctg gcgcgcgggg ctcgaggtgc ggttcgccgc cgagccgggg cgcgtcgatt
    17761 tccggccctt caacctgacc ttccggacga cgccggagga ggacccgctc ctcgcccgga
    17821 cggcgctcgc catcgcctcg cgaatggtcg accgcttcca gctccggcgg caggaccgcg
    17881 agatcctcct gacgctcacg cgagagcggc ggttcccgaa gggcgagcct ccccctcccg
    17941 agccgcggcc ggagggcgcc gtctcggccc gcgccgcgcg gccgcacgag attccgctcg
    18001 ccgcggggct cgcggccgcc cgccaccccg cgggacaggt cctcccggcg ttcggaagtc
    18061 ccgggacggt ctccgcgatg gcggcggcgg gggacctcga cgcggtcgtg gcggccgacg
    18121 cggccggtcg gctggcgggc gctctcttct tccgccccga gggcgaccgc ctcgtcgagc
    18181 tcctcggacc gttcctcttc caggagcccg ccgtggaggg cgccgcgcgc ctcctcgtcg
    18241 acgcgtgcct cgccgctctc ggacgcgccg gcaggaccgg agtcctcgcg aggaacccga
    18301 ccgacgcgat gccgccgaac gccctcgagc cgctcggccc cctcgacctt cgcggcgccg
    18361 acggcgtcgt ccgccgcgtc cacgcccgat tccgccagct cgacgaggac cccggaggga
    18421 tcgcgcacct ccacccgcgt ctcgagccgt tcgtgaggga ggccctccga cggctcgcct
    18481 tcgcgcggac cttccggctg ctcgagggcc ccgccgtgcc gcccggcgaa ggcacggcct
    18541 tcgcgaccga gatcgaccgg aggaacggac aggcccacct gaagccgctc tggctgggcg
    18601 ccgacgcgcg cgcgacgctc gacggcctcc tcgcggcgct ctcggcggag ggcctcgcga
    18661 acccgacggc cgagctggac ctcggcgagc cgtggcacgc gggcttcgcg ccggcgctcc
    18721 tcgacgccgg gttcgagccc cggctcgtcg tgccgtgggc ggcgacggga gacgtcctgc
    18781 tgctgcagcg gacgacggag ccttcggcgc gatgaccgcc tggaccctcg agccgctcgt
    18841 cccggagttc gtgcggcagt tcgaggcgta cgtcccgagc cggcccgacc cggagctgat
    18901 ggcgctctac ggcgtcccgc gccttttccg cctcaacaac aacgagaacc ccctcggccc
    18961 tcccgccgcc gcgcaggagg tcctgaggcg cttcccgccg ccgcgcgcgg cggtctaccc
    19021 ctccggcgac tccttctacc tccgccgccg gctcggggcg gcgctcggcg tcgacccgga
    19081 gcaggtcctc gtcggcaacg gggcgaacga ggcgatcggc ttcgtgatca aggcgttctg
    19141 ccaggagggg gacaacatcg tcaccgccga ccggaccttc gccgtctacg agtggacggc
    19201 gcgcttctcc ggaatcgacg cccgcctcgt ccccctctcg gacctcgggt tcgacgacga
    19261 ggggatgctc gcgcggatcg acgcgcggac gaaggtcctc ttcgtctgca acccgaacaa
    19321 cccgaccggc accttctggg accgggagcg gctccggcgc ttcgtcgaga gagtcgacgg
    19381 gcgggccgtc gtcgtggtcg acgaggcgta cgccgagttc gtcgaggacg cttcctaccc
    19441 cgacggcctc gccctcctgc ccgaccatcc gaacgtcgtc gtcttccgca cgttctcgaa
    19501 gatgtggggc ctcgccggcc tgcgcgtcgg ctacctcgtg ggagagagga aggtcgtcga
    19561 caccgtgcgt cgcacctgca ccgtctactc ggtcaacggc ctcgcccagg aagcggccct
    19621 cgccgcgctc ggggacgacg ggcacgtccc ggccacgcgc ctcctcgtcc gggaggagaa
    19681 ggcgtacctc gcaagcgagc tctcggcgat gggcctgacc gtcaacgccc gcgaggggcc
    19741 ctacgtcatg gtcgagcttc ccgtcagcga cacgctcgcc tacaggaggc tgatggcaga
    19801 gggggtcatg gtgaggacga tgaccggctt ccgcttcccc ggctggatcc gcgtcagcat
    19861 cggccgccac gacgccatgg aggcgttcgt tggggcgctt cggagggcgc tcgcgtgagg
    19921 gtcggcgagc cggcccgcat cggcgtcccg ctcccgcgcg ccgacgcggg cccgaaggcg
    19981 gcgggcgagc cgctctacgc cggcgacacc gtgccggacg gggcgctctg ggccggggtc
    20041 cgcagggcgg gcgtccctcg cgcgcgcctc gtctcgatcg acgtcggccg agccgccgcg
    20101 ctcgagggcg tcgtcgcggc cctgacgcac cgggacgtgg cgggcacgaa ccgacagggc
    20161 gtcatccggc gcgaccagcc cgtcctcgtg gacggggagg tgcgccacgc gggggacccg
    20221 ctcgccctcg tcgtcgccga gtcgcccgag gcgctcgagc gggctctcgc gctcgtcgac
    20281 gccgtcctcg agcctctccc gccgctgctc gacgccgccg aagcgctccg cgacggggct
    20341 cccgtcctcc atccggacca ccccggcgga aacgtcctcc tcgaggggcg tctcgtctcc
    20401 ggctcgggcg aggcggccga gaccggctgc gactccgtcg tcgaggtcct cttcgagacg
    20461 ccccgccagg agcacgcctg cctcgagacg gctgcgggct gggccgtcgc gacgccggac
    20521 ggcgccgtcg aggtgacctg ctcgacgcag accccctggc gcgaccgagc cgagaccgcc
    20581 gaggcgctcg gcctcgaccc cgcccgcctg cgcatcgtcg cgccgtacct cggcggcggt
    20641 ttcgggggca aggacggcct cacggtccag tcgctcctcg cgctcgccgc gctccacgcg
    20701 gggcaccgcc ccgtgcggat gcggtggggg cgggaggagt cgatcgtcgc cggagtgaag
    20761 cgccacgccg gccgcatgac ggtgaagctc ggcgcgacgg ccgacgggac gctccgcttc
    20821 gcctcggccg acgtcctcct cgacaccggc ccctacgacc acctcggcgg cgccgtcctc
    20881 gagctcgccc tcgagcacgc caccggcccc taccgcgtcc cgaacgccgt cgtcacgggg
    20941 agggccgtct acaccgacaa cccgatcggc ggcgccttcc gcggcttcgg cgtgacgcag
    21001 gtgaccgccg ccgtcgagca ggccgtcgac ctcctcgccg accgcctcgg cctcgaccgc
    21061 ctggagatcc gcaggaacaa cgccctgtcg cgcggggacg tcaccggcac cggcttcacc
    21121 ctcctctgct ccgtcggcct cggcgacgcc ctcgacgccc tcgcccgcca cccgctcttc
    21181 acggggcgcg aggcgtggaa ggcggaggcc ggccccttcc ggcggcgcgg cgccgggatc
    21241 gccgccctgt ggcacggcgc gggctacggc ccggtcgtcc ccgacgtcgc caacgcgaag
    21301 gtcgagcttc tcccctccgg gcgtttccga gtcttctgcg gcgtcgccga catggggcag
    21361 gggaacgtcg ccacgaacgc tcaggtcgcc ggcgccgtcc tctgtcagcc gcccgaggcc
    21421 gtcgagctcg tcctccccga cacc
//
LOCUS       DAOTEZ010000016        21092 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_1649_length_21092_cov_5.307743, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000016 DAOTEZ010000000
VERSION     DAOTEZ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 21092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21092
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1649_length_21092_cov_5.307743"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="P5164_01555"
     CDS             complement(<1..948)
                     /locus_tag="P5164_01555"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRY42606.1"
                     /translation="MKRLALVLPLALLAWTGCSSTPRTGAAAPAPEASADTAKEAAAR
                     EELAERERKTVEAARMLAGLPPLSEEDGSEREEWIIFTDPETGKKLQRIPKNPSLRVV
                     DGKLRHTQLNPAMFTLDLVRQDEGFYYVAAPEEPSAKPPVDEDATAEGLRPIVEVDPL
                     EYEVVTPAPSKVRLRLEERSEGLPVSGFWRSNLAVADLDGDKRPEIVTTPPRLGMGTL
                     QIFRYDGARWSAVEPVIEGDESLNYAYGGVAVADMDGDGRPDVVSVGHGSGPFVSYNL
                     GGFRFRREARSLPRRMSGRAVSAGDLDGDGRADLVAISDE"
     gene            complement(1019..1786)
                     /locus_tag="P5164_01560"
     CDS             complement(1019..1786)
                     /locus_tag="P5164_01560"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY42607.1"
                     /translation="MRRNVFLLSELVRRDLAARYAGSFGGAAWAVLNPLILCGLYTLV
                     FSVIVRVPPPADFRGTYAEFLLAGLLPWLGLQDALVRGASSVTEQANLVKKIPFPVEI
                     LVLSSLGSALILQAASLVVFAAVSVPFGSGELHLAPLAGALLFQLLLLAGPMFALAAG
                     SVLFRDLAQLLGPLLTVAFYLTPILYPEELVPASLAPGMALNPLRDLAGLFRAGLLGT
                     TAPPLERLLVWGAGLAVLAFFGHRFFARSRRAFADVL"
     gene            1871..2353
                     /locus_tag="P5164_01565"
     CDS             1871..2353
                     /locus_tag="P5164_01565"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY42608.1"
                     /translation="MSTSARRSQIIVVGQKIRQIRKSRRLTQADLASRIGVTQSDLSR
                     MENGEYKVGLDTLFRILQVFELSMSRFFEEPAVETAESEDAPAPDTVPESQQLVADFE
                     ELDEDSRREVLDFIAFKKIQQQNRQPRWRPEEAPQEPGPAVAGPLLPFGRPGGPGRAE
                     "
     gene            2368..3291
                     /locus_tag="P5164_01570"
     CDS             2368..3291
                     /locus_tag="P5164_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY42609.1"
                     /translation="MTRSTPDAAALRSRSEALRSDAVAFLEKGEFAASLGQYDQALDA
                     ARGTGDETFVDWIFACRAAAAAEAGPAESELLELKRILLRGRDAQTSFRAAYTAARIY
                     ELRRDYRKAGFYARLAGDHAAVVGDPLLLAGAQSTLGTIHAVDSRFEEAAECYRRALE
                     TSAPPARVSAVNRAVWQDNLGYCLLALDQTSEGLALVHEALDTLERAGAAAHTVYPLM
                     DLCFGYLKCDRYEESRYFGEACLERAPLRNDETVERNALYLLGEASHLGGDEDSARDY
                     FDRLASLYPDFRNLRAYLEVFDFRNVINLRS"
     gene            3302..4402
                     /locus_tag="P5164_01575"
     CDS             3302..4402
                     /locus_tag="P5164_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42610.1"
                     /translation="MKKKRFAPLALAAAALLGAAFLAPMPARAESAAVVSPDGTLYEV
                     FPARYGDVVVVPEGSPDANLQVLALRVTPSGQPGHLELVDGTVDANAEGSVSIELEET
                     TRTLFVAYTKANGLMADMHVAVRRDGAWSGQDILSNRGFYLSYNPKVVVTRQSYFDFD
                     AEGMPVTKSRSIFSLVWWEESGPTQARYAPIFVEDGELRVDAVQPWNLNELAGAAGPT
                     DSTGLPLSAYTFPSVQADPSTNGGVIVSFANLATHTQQVLRISFVDDLVKLSPPGATR
                     VPDDVWARGHVPIGRSGGAGRIPAIMDTGALVGATISSSGITTFHWVEGGAMNFVRSD
                     DAEGAVTRRIALRDDLSAEKALRLVRSLAEKN"
     gene            complement(4517..5542)
                     /locus_tag="P5164_01580"
     CDS             complement(4517..5542)
                     /locus_tag="P5164_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HRY42611.1"
                     /translation="MAMKDPYEVLGVPRTAGDADVKKAYRRLARKFHPDVNPGDSSAS
                     KRFQEIASAYEILKDPKKKERYDRFGDTGEPPDAASGAPRPGAWGAGPFGDASRSTPF
                     GRGGGGAGGGFRWSGDFSDLFSDLFSGAGGGIGVGEDEDAAANLTIPFRDAVLGGTVS
                     FSARIPRRCAHCRGTGRAAKAACPACHGSGALVETESLSVRVPAGVETGSRIRVAGKG
                     RTENGDLYLSIAVEPHPYFGREGDDIVAEVPVTVPEAYLGAEIEVPTVRGPVRARIPA
                     GTLSGQRFRLKGRGIENLRTGTTGDHYYRVRVIAPTVQSEEGRRLVEALGKLYPRDLR
                     ADLPRGI"
     gene            5637..6878
                     /gene="glgC"
                     /locus_tag="P5164_01585"
     CDS             5637..6878
                     /gene="glgC"
                     /locus_tag="P5164_01585"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HRY42612.1"
                     /translation="MLQHRDVLSIVLAGGMGERLYPLTRDRAKPAVPFGGRYRIVDFV
                     LNNFVNSGLMKIKVLTQFKSNSLIEHLVRAWRLNPEIGQFVDPVPAQMRRGPHWFRGT
                     ADAVYQNLDLIFDEEPSQVCVFGGDHVYVMDVNPMLAFHEDGGHDLTIAAFPVPVSEA
                     TRFGVLEVDDGGRVTGFEEKPREPREIPGAPGFALASMGNYIFRPKVLREALENDALQ
                     DTSHDFGRTIIPALLDGAALVYAYDFSASRVPGDEELAPYWRDIGTVDSYWEASMDLI
                     SVSPPLNLYNPRWSLKSSSPHLPPAKFVFADAASNRIGITTDSMVADGCIVSGGTTHR
                     SILFPRVRVNSFARVEESVLMDGVDVGRHARLRRVIVDKGVKIPPGTVIGFDPESDRK
                     RFHVSEGGVVVLPKGVEVPSS"
     gene            6951..7400
                     /locus_tag="P5164_01590"
     CDS             6951..7400
                     /locus_tag="P5164_01590"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRY42613.1"
                     /translation="MDEIDRQILDMLQRDGKVSQARIAESVGLTTPSVNERIKKLETK
                     GFIRRFAALLDPRQVGMAAVAHVDVQLEHPSFERAFLDEIENLIPVQECYSISGDYGY
                     RLKVRARDLEQLEALLRERIQTIRGVARTRVDLSLSIKKDSTLLPVT"
     gene            complement(7774..8343)
                     /locus_tag="P5164_01595"
     CDS             complement(7774..8343)
                     /locus_tag="P5164_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42614.1"
                     /translation="MNAQCRPRRWRAVSLVLLSAAFLATAPAAPAQTFAIGAGGGLLN
                     DEGSAESLKSFSTGAGYGFAEIALDPGILLQARYTRMKLPSSAADGPDIDVDAATLSV
                     AYLFREDWWQAGFVAGGGGYWLGPKEPGPGQVALEQSTARFGWHGGVLTIFALGRRAD
                     LRLEAVGHLLRDDDRRKPIVLSAALAWKF"
     gene            8435..9199
                     /locus_tag="P5164_01600"
     CDS             8435..9199
                     /locus_tag="P5164_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRY42615.1"
                     /translation="MAGAPWGWPLKGGSAILQLVLPSCCAVCGTPRRGVGGGGVCAAC
                     WAALPLLDPETACETCALPGAAPLCRTCAVTLPPVARTIAVARYESSVRRVVHALKFR
                     GHDILASRCGALMAAAARSARLHESLSAVVPVPSTSQRNRDRGYDPAALLAEEVGRRL
                     GAPFRPLLSRVRETPPQSRVPASRRRDNVRGAFAASARARGESLLVVDDVMTTGATAF
                     EAARALRGAGAAAVALLVLARTPEAGPAPRGSTEDA"
     gene            9196..10110
                     /locus_tag="P5164_01605"
     CDS             9196..10110
                     /locus_tag="P5164_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HRY42616.1"
                     /translation="MSSIFDRLVVGTLPLVPKPVIFRFARRYIAGLTLDDAVRTVREL
                     EAEGAMSTIDVLGEAVTRREQTEATRDEYLRVLDVLSAQRLPANVSVKPTALGLSIDP
                     KLVRENFRAICRKAAESETFLRIDMEDSPYTESTLALALELKEEFPNVGVVVQAYMRR
                     TLRDMDRLIAARMNVRICKGIYVEPRALAYKDRQVVIESFAAIVDKHLSAGCYAGIAT
                     HDEACVQRALATIDRLKLTPDRYEFQMLLGVDPLLRRTILAAGHRLRVYVPYGRDWYK
                     YSVRRLKENPSIAGHTMRSILGIGPAQS"
     gene            10371..12134
                     /locus_tag="P5164_01610"
     CDS             10371..12134
                     /locus_tag="P5164_01610"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRY42617.1"
                     /translation="MLRADGAPLAGVSLVVRGLTGAAASVVRVLKTDAEGTFCLADAR
                     PGVYSVLAAVPGFRSATAQVLHRASGESLSFVRLDLDRDRRGVLPAGPAGALDPWTAR
                     AVLEGDVLRDEGPVDAPAPSTADARVPAAPASASASIPLPLRGTVRSLQGFGAEGSES
                     LSQTALDVRGSLGKDATWGLSGEFDRLTAAGGEQVGRTSRVALDVLPARGHSIHVSSR
                     RSELPSFDSPDARLDAHSVDWAAEPGRGSRASVSARFVSHRNLEPALLPTPLFRGEGR
                     ALAVDARYRSDVGEGRFVRLHVGYRSELAEATVAHAARSGREARVGGAAGVRLLDALL
                     VEAGGTGDYSDASRGLTPEVTVSLEALDGVTVYGFASRRFEQRDPNVPLYGLVGIDAA
                     DLVRATRSHYRAGTRLESGDGTTRLELETSRREISDAFQLLLDSEMIARVDALYLFPG
                     DVADEASGSFSFVLGPGLAARLALTAGRVRGDGALPGALRNEARYQVSSARIDVLPSG
                     TAVSIRYRLLEQELGRNASYRNGRESVDVTIAQEVPIPLLRALGSRWQALFSMALENR
                     SEGDSPVRPNRQMSGGLSLTF"
     gene            12286..15033
                     /locus_tag="P5164_01615"
     CDS             12286..15033
                     /locus_tag="P5164_01615"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY42618.1"
                     /translation="MRNLRRRSTRILRWGFLGVFLALLVPIGFTAARKIATTGQTGLL
                     LTPEESGFRVRVPGTAAAAGLLPGDLLLLVDGAEARALPDPVRSIEGDVELTILRDGQ
                     LLRLSGRVDASPWDTRYFLLLLVGTAFVVAAGLVLRTAPDEADPAGAFHFAGFAACAA
                     AVLVLTPAPPFDALFRFGTLLEDLARAFLPAFLLAFVFRFPRRAARAPGWLFFLPAVA
                     LAALSAATYLDPPAADADATALVLRLDRAQQAAMLLGVVVAVLRLVTLASRRLDLLAE
                     KQVRFLLAGTAGGLLPVVLLDFLPRLLGAPIPVVSSFSIVPLVLVPVAFVAALTRYRL
                     WDVEILTRETVALLGAAFLGAGLFSLAQVVDLGRVLPGIPYGRGIVETGAGLLIALTF
                     FPVRRGLSTALARVQYGERFGEREGLLALVRDLARPRRLSEIGPLLAERVTNGLGVPR
                     ATLLLALPDGRFDASALDGGDPLPAAELPADAATRTLRLSRLAFTERPTAAVARLRHA
                     GLRTIAPLALSGRLLGLFAVADRDGRDPLSAEDVELLETVLASAALAVDHARLYDELE
                     AQADRYRKLKEFHEDVVTGSPAAIAATDESGRITSANPAFEALFGSGSADLVGRRAEE
                     VLPEAVLSAHAPARVEVTVGGELRAIDVAVSPFPGASEGSAARVWVLSDATELARLER
                     SLAEKERLSALSSLSAGVAHEVNTPLTGVASYARLLLDDTPADDPRRPVVEKIERQAF
                     RAARLVGSLLDLARGRPRDLSPLAPRDLVAEACRALEDEVRGRRVRLDVDLPESLPNV
                     LGHADALVQVLVNLLKNALEAVAAPKEGRPGPGSVRIAAAPLAGSVVFSVEDDGPGLS
                     RPEQERAFAPFHTTKGGQGGVGLGLAIAGDIIRAHGGSLAVDSTPGRGARFTVSLPAQ
                     T"
     gene            15030..16418
                     /locus_tag="P5164_01620"
     CDS             15030..16418
                     /locus_tag="P5164_01620"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRY42619.1"
                     /translation="MKVLIVDDEPVVREVLRTVLSRAGYATSEAATAAEGLALFADPA
                     VDLVLLDLMLPDRPGLSLLPELREKRPDVPVVVVTAYSSVESAIVAMKEGAFHYVPKP
                     FRNEEVVHLVAQALEKRRLVAENLALKARLDSGTDFPEMVGRSAPMEKVFQTIRQAAP
                     ARSTILVTGESGTGKELVARALHRLSQRASGPFVTVHSGALPPDLLESNLFGHVRGAF
                     TGAVAEKKGLFKVADGGTIFFDEIGTVPLETQAKLLRVLQEREFLPVGAVEPVRSDVR
                     VVAATNADLARLVEEGKFREDLYYRLCVISIGLPPLRERKEDVPLLVEALVSRAAREN
                     DRPVPPVTPAAMKALLDHDWPGNVRELENVLERAVVLGGGIIDLDQLPEAVRRSVPRP
                     EALPTDGVAFYDAVTRYEKALLSSAMQRAGGVQKEAARLLELSPTTLNEMLKRHGMTS
                     RRPSRATEPEPA"
     gene            16415..19132
                     /locus_tag="P5164_01625"
     CDS             16415..19132
                     /locus_tag="P5164_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HRY42620.1"
                     /translation="MRLPLAPLLALLLALPTAAAGDDDPVFSSRGVPSGRVLAESERD
                     PSVPPPAVGLGHRIGDALTPHDALVRYVRLLSTASRRVSTMVYGTTPEGRELLRVAVS
                     SPENLARLDAISADLARLADPARTSKEEARAIAERSPAVAWIACGIHGNEVAGPEACA
                     ALLYTLAASREADVQKLLSALVVVLDPCANPDGHERHVSWWRSVAGAEPDPDPRALEN
                     EPPWPSGRGNHFGVDLNRDWAWGTQPETRARIAALRALPPQLYVDVHEMGPEQSYFFP
                     PPADPVHPRVPDSTRAWIEAFGAANAEAFDERGWGYFSREVFDLFYPAYGDSWPVFRG
                     MVGMTFEVGGKGGLAHRRRDGAVVTLKERALKHWTALGATLRTAAAGRARILADYAEF
                     FRSSVAEERRAFVVPGGQDPSRLSELASLLALQDVEVKVTTRALPNVPAKGETLPSGS
                     LVVETAQRFGRFAQVLLESQAALAPRFLEEERRRLLSEEDERFFDVTAWSLPIAFGLE
                     ARLLPGVGPLANATAPWRPAEPASPRGGRFGWLLPGDDATSRRCAAMLAAAGVRVSAT
                     TKEARAASGPVPAGSYLVAREENRDVPGLDDLVAGVVGAAKARPRPLETAATLSGPSL
                     GSSSLLRLKSPRVALLSGDGADPAGIAFLRHALEERLGVRPTLRRPESVGSEGFDGFT
                     ALVVPHGNARFRRSLLEEGTAEAIRRFVDGGGVLVAIRGGATALREAPLALSKVARWE
                     PPSTEPEAPADTPEGAAAEAPPPAKPSEPPPPTPEAALERDLERRRLPVPGAALKTQA
                     LPGHPFLFGLAAAPAFLVLDGEPPLRLPDALANVVTIAPDEPLLSGFGWKEALERWKG
                     AAVVQVEEAGRGRVVSFGADPVFRGVWRGSESVFLNAVLLGPSLGAKGW"
     gene            complement(19138..20793)
                     /locus_tag="P5164_01630"
     CDS             complement(19138..20793)
                     /locus_tag="P5164_01630"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRY42621.1"
                     /translation="MLVLTIHSPGEPPRRLRVDRPELTLGRASTNDVFLPDRTLSRAH
                     ARLTRGPGGVVLVDLGSRNGTQLNGARIAEPVPVRAGDRIVLGETTIEVSEEASSSAQ
                     VVIEAGGGPIEKTMISTASAAAGLARSAARELTDAAELKRLATSLQILNEVSLALLTD
                     IPLADLSSLILEKLFAYLEPDRGLLMLRDEAGSLQPEALKLAEGIDPADLRLSRTLVE
                     AVTERREGVLMIDTRTDAKLGTADSIRLQGITSCLAAPLLAGETVLGLVYLEARLGRK
                     SFTEEDLRLLTSLANTAAIKIQNQRLQETAAAKQRIEREMALAWDVQRRLFPEVPPEL
                     PSSELLGRTLPSRTVSGDYYDFFVRGDGTVDVVVADVCGKGMAASILAASVQSAFQAW
                     AAEHFPPDRLCARLNDLVYRRTSPEKFVTFFAALYDPETGSIVYSNAGHNPGIVLRAG
                     GGHELLPAQGPPLGLFAGRSYGSGALTLAPGDLLLLYTDGLTEAANPEDEEFGLERLV
                     EAARSCKDRPLEAVEEELRERLAAFAAGVPFHDDRTVVLLRRR"
     gene            20938..>21092
                     /gene="rpmE"
                     /locus_tag="P5164_01635"
     CDS             20938..>21092
                     /gene="rpmE"
                     /locus_tag="P5164_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRY42622.1"
                     /translation="MKEKIHPTYQEVTVHCSCGATWKTGSTMKELRLEICSNCHPFFT
                     GKAKLIDT"
BASE COUNT         2863 a         7973 c         7163 g         3093 t
ORIGIN      
        1 ctcgtccgag atcgcgacga ggtcggcccg cccgtcaccg tcgaggtccc cggccgagac
       61 cgcccgcccg ctcatccggc gcggcaggct gcgggcctcg cgacggaacc ggaagccgcc
      121 gaggttgtag gagacgaacg gccccgaacc gtggccgacc gagacgacgt cgggccggcc
      181 gtcgccgtcc atgtccgcga cggcgactcc gccgtaggcg tagttcagcg actcgtctcc
      241 ctcgatgaca ggctcgacgg cgctccagcg cgcgccgtcg taccggaaga tctggagcgt
      301 ccccattccg agccggggcg gggtcgtcac gatctcgggc cgcttgtcgc cgtcgagatc
      361 ggcaacggcc aggttggagc gccagaagcc gctcacgggg agcccctcgg acctctcctc
      421 gaggcggagg cggaccttcg agggggccgg cgtgacgacc tcgtactcga gcgggtcgac
      481 ctcgacgatc ggccgcagcc cctcggcggt cgcgtcctcg tcgaccggag gcttcgcgga
      541 gggctcctcg ggcgcggcga cgtagtagaa gccctcgtcc tgccggacga ggtcgagcgt
      601 gaacatcgcc gggttgagct gcgtgtgcct cagcttgccg tcgacgacgc ggagggacgg
      661 gttcttcggg atccgctgca gcttcttccc cgtctccggg tcggtgaaga tgatccactc
      721 ttcgcgctcc gagccgtcct cctcggacag gggcgggagg cccgcgagca tgcgcgcggc
      781 ttcgacggtc ttgcgttccc gctcggcgag ctcctcgcgg gcggccgcct ccttcgcggt
      841 gtcggccgac gcctccgggg ccggcgccgc ggcgccggtt cggggggtgg aagagcagcc
      901 cgtccatgcg agcagggcga ggggaagtac gagggcgagt ctcttcaagg cgtctcctgg
      961 ggcgcacgag agccgcgaac cttcggctcg cggcccgatg ggaggagtct cccgatgatc
     1021 agaggacatc ggcgaaagcc ctgcggctcc gcgcgaagaa tcggtggccg aaaaatgcta
     1081 gcacggcgag gccggcgccc cagacgagga gccgctcgag gggcggtgcc gtcgtgccga
     1141 gaaggccggc ccggaagagc cccgcgaggt cgcggagcgg gttgagcgcc atccccggcg
     1201 cgagcgacgc cgggacgagc tcctccggat agaggatcgg cgtcaggtag aaggccacgg
     1261 tcaggagagg accgaggagc tgggcgaggt cccgaaacag cacgctgccc gccgctaaag
     1321 caaacatcgg gccagcaagc agcagaagct gaaaaaggag tgcccccgcc agcggagcga
     1381 ggtgcagctc tccggaaccg aacgggacgg acaccgccgc gaagacgacc aggctcgccg
     1441 cctggaggat cagggccgag ccgaggctcg agaggacgag gatctcgacg gggaatggaa
     1501 tcttcttcac gaggttcgcc tgctccgtga ccgagctggc gccgcgcacg agcgcatcct
     1561 ggagcccgag ccaggggagg agcccggcca ggagaaattc ggcgtaagtc ccgcggaagt
     1621 cggccggggg cggcacccgg acgatgaccg agaagacgag cgtgtagagg ccgcagagga
     1681 tgagcggatt caggaccgcc caggcggcgc cgccgaagct tccggcgtat cgagcggcca
     1741 gatctcgccg gacgagctcg gagaggagga agacgtttcg tctcaaatct gatccggcgg
     1801 gcgcccggct cgggacgaac tgcttgacag ggacccggga gggcttaacc ttaggttcgg
     1861 gttcgtgtcg atgagtacca gcgcgcgtcg gtcgcagatt atcgtggtgg ggcagaaaat
     1921 ccgtcaaata cggaagagcc gccgcctgac ccaggccgac ctcgcgagcc gcatcggcgt
     1981 cacccagtcc gacctctcgc gcatggagaa cggcgagtac aaggtcggcc tcgacacgct
     2041 cttccggatc ctccaggtct tcgagctctc gatgagccgc ttcttcgagg agccggcggt
     2101 cgagacggcg gaatcggaag acgccccggc tccggacacc gtcccggagt cccagcagct
     2161 cgtggccgac ttcgaggagc tcgacgagga ctcgcgccgc gaggtcctcg acttcatcgc
     2221 gttcaagaag atccagcagc agaaccgcca gccccggtgg cggcccgagg aggctccgca
     2281 ggagcccggg ccggccgtcg cgggtcccct tctcccgttc gggcgccccg gcggccccgg
     2341 tcgcgccgag tgaggtcgag aagcgagatg acgagatcga caccggatgc cgcagcgctc
     2401 cgcagccgtt ccgaggcgct gcggtcggac gcggtcgcct tcctggagaa gggcgagttc
     2461 gcggcctcgc tgggccagta cgaccaggcg ctcgacgccg cgcgcggcac gggcgacgag
     2521 acgttcgtcg actggatctt cgcctgccgc gcggccgctg ccgccgaggc cggccccgcg
     2581 gagagcgagc tcctcgagct gaagcggatc ctcctccgcg gccgcgacgc ccagacgtcc
     2641 ttccgcgccg cctatacagc ggcccgaatc tacgagcttc gtcgcgacta ccggaaggcg
     2701 ggcttttacg cgcggctcgc cggcgaccac gccgccgtcg tgggcgaccc gctcctcctg
     2761 gccggtgccc agagcacgct cgggacgatc cacgccgttg acagccgttt cgaggaggca
     2821 gccgagtgtt atcggagagc tctcgagacc tcggcgcctc cggcccgcgt ctcggcggtg
     2881 aatcgcgccg tctggcagga caacctcggt tactgtctcc tcgccctcga ccagacgtcg
     2941 gagggcctcg cgctcgtcca cgaggcgctc gatacgctcg agagggccgg cgccgcggcg
     3001 cacaccgtct acccgctcat ggacctctgc ttcggctacc tcaagtgcga ccggtacgag
     3061 gagtcgcgct atttcggcga ggcctgcctc gagcgcgccc cgctgcgcaa cgacgagacg
     3121 gtcgagcgca acgccctcta ccttctcgga gaggcgagcc atctgggcgg cgacgaggac
     3181 tctgcccgtg actacttcga tcgtctcgct tccctctacc ccgacttccg gaacctcagg
     3241 gcctacctcg aggtcttcga cttccggaac gtcatcaacc tccgatcgtg aggtgagcgc
     3301 catgaagaag aagcgcttcg cccctctcgc cctcgccgcc gcggccctcc ttggagcggc
     3361 gttcctcgcg ccgatgcccg cgcgggccga atccgccgcg gtcgtctctc ccgacgggac
     3421 gctctacgag gtcttcccgg ctcgctacgg cgacgtcgtc gtcgtccccg agggttcgcc
     3481 cgacgcgaac ctccaggtgc tcgccctgcg ggtgacccct tccggtcagc cgggacacct
     3541 cgagctcgtc gacggaacgg tcgacgctaa cgccgagggc tcggtctcga tcgagctcga
     3601 ggagacgacc cgcaccctct tcgtcgccta cacgaaggcg aacggcctca tggccgacat
     3661 gcacgtcgcc gttcggcgcg acggcgcctg gtcggggcag gacatcctct cgaaccgcgg
     3721 cttctacctc tcctacaacc cgaaggtcgt cgtgacgcgg cagtcgtact tcgacttcga
     3781 cgcggagggg atgcccgtca cgaagagccg ctcgatcttc tccctcgtct ggtgggagga
     3841 gagcgggccg acgcaggccc gctacgcgcc gatattcgtc gaggacggcg agctgcgcgt
     3901 cgacgccgtc cagccctgga acctcaacga gctcgccggg gccgccggcc ccaccgattc
     3961 gaccggcctc ccgctctccg cgtatacgtt cccgtccgtc caggcggacc cttcgacgaa
     4021 cggcggcgtc atcgtctcgt tcgcaaacct cgcgacccac acgcagcaag tcctccggat
     4081 cagcttcgtc gatgacctcg tgaagctcag cccccccggc gcgacgcgcg tcccggacga
     4141 tgtctgggct cgcggacacg tcccgatcgg ccggtcggga ggcgcgggac gcattcccgc
     4201 catcatggac accggcgcgc tggtcggcgc gacgatctcg tcctccggca tcacgacgtt
     4261 ccactgggtc gagggcggcg cgatgaactt cgtccgcagc gacgacgccg agggcgcggt
     4321 cacgcgccgc atcgcgctcc gtgacgacct ctccgccgag aaggcgcttc gactcgtccg
     4381 gtccctggcc gagaagaact gacgtccgta cgacagacgg ccccgtcgaa acgccccggt
     4441 ccgccggggc gtttctattc cgggagggac cgccgcggcg cgccgggaag ggctcggcgc
     4501 gcgacggcct cgcgtctcag ataccgcgcg gcaggtcggc ccggaggtcg cgggggtaga
     4561 gcttcccgag cgcctcgacg agccggcgcc cctcctcgct ctggaccgtc ggggcgatca
     4621 cccggacccg gtagtagtgg tcgcccgtcg tccccgtccg gaggttctcg atgccgcgcc
     4681 ccttcaaccg gaatcgctga cccgagaggg tccccgcggg gatccgcgct cgcacaggcc
     4741 cgcgcaccgt cgggacctcg atctcggcgc ccaggtaggc ctccggcacc gtcaccggca
     4801 cctccgcgac gatgtcgtcc ccctcccgcc cgaagtacgg atggggctcg acggcgatcg
     4861 agagatagag gtcgccgttc tccgtccgcc cctttccggc gacgcggatc cgcgatccgg
     4921 tttcgacacc cgcggggacc cgcacgctga ggctctcggt ctcgacgagg gcgcccgagc
     4981 cgtggcaggc cgggcacgcg gccttcgccg cgcggcccgt cccccggcag tgcgcgcacc
     5041 ggcgcggaat ccgggccgag aacgacacgg tcccgccgag gacggcgtct cggaacggga
     5101 tcgtcaggtt cgcggcggcg tcctcgtcct cgccgacgcc gatcccgccg ccggcgcccg
     5161 agaagagatc ggagaagagg tccgagaagt cgcccgacca gcggaatcct cctccggctc
     5221 cgcctccccc tctgccgaac ggcgtgcttc gggacgcatc tccgaacgga cccgctcccc
     5281 aggcccccgg gcgcggcgcc ccggaagcgg cgtccggcgg ctcgcccgtg tccccgaacc
     5341 ggtcgtagcg ctccttcttc ttcgggtcct tcaggatctc gtacgccgag gcgatctcct
     5401 gaaatcgctt gctcgcggaa gagtcgccgg ggttcacgtc gggatggaac ttccgtgcga
     5461 ggcgccggta ggccttcttg acatcggcgt ccccggccgt acgcggaacg ccgagcacct
     5521 cgtaggggtc cttcatcgcc atgccgggaa ggatagaaga aacgggaacg gacgcgcagg
     5581 aagccttcgg ttccgaggct cctagaatgg gccgaacgag gtgctccgcc gtgaagatcc
     5641 tccagcacag ggacgtcctc tcgatcgtcc tcgccggcgg catgggcgag cgcctctacc
     5701 cgctgacccg cgaccgcgcc aagcctgcgg tcccgttcgg cggccgctac cggatcgtcg
     5761 acttcgtcct caacaacttc gtcaactcgg gcctgatgaa gatcaaggtc ctcacgcagt
     5821 tcaagtcgaa ctccctgatc gagcacctcg tgagggcctg gaggctgaac cccgagatcg
     5881 ggcagttcgt cgaccccgtc ccggcgcaga tgcggcgcgg accgcactgg ttccggggga
     5941 cggccgacgc cgtctaccag aacctcgatc tgatcttcga cgaggagccg tcgcaggtct
     6001 gcgtcttcgg cggcgaccac gtctacgtga tggacgtgaa cccgatgctc gcgttccacg
     6061 aggacggcgg ccacgacctg acgatcgcgg cttttccggt gcccgtctcc gaggcgaccc
     6121 gcttcggggt gctcgaggtc gacgacggcg gccgggtgac cggcttcgag gagaagccgc
     6181 gcgagccccg cgagatcccc ggagcgcccg gcttcgcgct cgcctcgatg gggaactaca
     6241 tcttccggcc gaaggtcctc cgtgaggcgc tggagaacga cgcccttcag gacacgtcgc
     6301 acgacttcgg ccggacgatc atcccggcgc tgctcgacgg tgccgcgttg gtctacgcct
     6361 acgacttctc cgcgagccgc gtcccgggcg acgaggagct cgcgccgtac tggcgcgaca
     6421 tcgggaccgt cgactcctac tgggaggcgt cgatggacct catctcggtc agtcccccgc
     6481 tgaacctcta caacccccgc tggtcgttga agagcagctc cccgcacctc ccgcccgcga
     6541 agttcgtctt cgccgacgcg gcgtccaacc ggatcgggat cacgacggac tcgatggtgg
     6601 ccgacggctg catcgtctcc ggcgggacga cgcaccggtc gatcctcttc ccgagggtga
     6661 gggtcaactc gttcgcgcga gtggaggaga gcgtcctgat ggacggcgtc gacgtcgggc
     6721 gacacgcccg cctccgccgg gtgatcgtcg acaagggagt gaagatcccg cccggaaccg
     6781 tcatcggctt cgacccggag agcgatcgga agcgcttcca cgtctccgaa ggaggcgtcg
     6841 tcgttctccc gaagggagtc gaagtcccct cgtcctgaac gaaagagctt gcattcgccg
     6901 acgctcctcc atataaagtt cggaggtttc gacccctctc cgaatatttt atggatgaga
     6961 tcgaccgcca gatcctcgac atgctccagc gcgacgggaa ggtctcccag gcgcggatcg
     7021 ccgaaagtgt gggtctgacg actccgtccg tgaacgaacg gatcaagaag ctcgagacga
     7081 agggattcat ccggcggttc gccgcgctcc tcgacccgcg gcaggtcggg atggccgcgg
     7141 tcgcgcacgt cgacgtccag ctcgagcacc cgagcttcga gcgggccttc ctcgacgaga
     7201 tcgagaacct gatccccgtc caggagtgct actcgatctc cggggactac ggctaccgcc
     7261 tcaaagtgag ggcgcgcgac ctggagcagc tcgaggcgct cctccgggag cggatccaga
     7321 cgatccgcgg cgtggcccga acgagggtcg acctctcgct gtccatcaag aaggactcca
     7381 ccctcctgcc cgtcacctga gccggcctgc gaggccgccg ggtgtcattt cggtcgccgc
     7441 ggatgccgaa atgcgccacg cccgggctcg atttgggacg gggcgtcccg gcgccgccgg
     7501 gatcccttcc ggcaacccgc atttcaagcg ggttcaccgt cccgagcgtc ctcgaaatcc
     7561 ggtggcattc cgattgcgta tagagaggca ggagatcgga aaacacgctc cacgccaggt
     7621 ggcgtcagat cttcagaacc tgcccgatgc cccgaaggct ccccgcctcc gggccctcct
     7681 acacaaaccc ctctcttcag ccccaccagg aaaatcccac cgccgcactt cccgcccgac
     7741 ccccgaggtc gggcgggaac cccgccattc cgatcagaac ttccaggcca gggccgcgga
     7801 caggacgatc ggcttgcgcc ggtcgtcatc ccggagaagg tgcccgaccg cctcgagccg
     7861 caggtccgcc cggcggccga gcgcgaagat cgtcagcacg ccgccgtgcc atccgaagcg
     7921 ggcggtgctc tgctcgaggg ccacctgccc cggcccgggc tccttcgggc caagccagta
     7981 accgccccct ccggcgacga agccggcctg ccaccagtcc tcgcggaaca ggtaggcgac
     8041 ggaaagcgtc gcggcgtcca cgtcgatgtc ggggccgtcc gcggcgctcg acgggagctt
     8101 catccgcgtg tagcgcgcct ggagaaggat ccccgggtcg agcgcgatct cggcgaagcc
     8161 atagcccgcg ccggtcgaga agctcttgag gctctcggcc gagccttcgt cgttcaggag
     8221 gcccccgccc gcgccgatcg cgaaggtctg ggccggagca gcgggcgcgg tcgcgaggaa
     8281 cgcggcggag aggaggacga gggacacggc gcgccaccgg cgcggacggc actgggcgtt
     8341 catccggccg cagcatagag gggcgcgaaa tcgagcgtca acgtgcatgt ccggcttacg
     8401 gaggccgatc ggccgtcgtc tagaatcccc tcccgtggcg ggtgccccgt ggggctggcc
     8461 cctgaagggc gggtcggcga ttcttcagct cgtccttccg tcgtgctgcg ccgtctgcgg
     8521 caccccgcgc cggggcgtcg gaggcggagg cgtctgcgcg gcctgctggg cggcgctgcc
     8581 gctcctcgac cccgaaacgg cctgcgagac ctgcgccctc cccggtgccg ctccgctctg
     8641 ccgcacctgc gcggtcacgc tcccgccggt cgcccgcacg atcgccgtcg cgcgctacga
     8701 gagctccgtg cggcgggtgg tgcacgccct gaagttccgc ggccacgaca tcctcgcctc
     8761 ccggtgcggc gcgctgatgg cggccgcggc ccgctcggcg cgccttcacg agagcctgtc
     8821 ggccgtcgtt ccggtcccgt cgacctcgca gcggaaccgc gatcgcggct acgaccctgc
     8881 cgccctgctt gcggaagagg tcggccggcg cctgggagcg cccttccgtc ccctcctttc
     8941 ccgcgtgcgc gagactccgc cgcagtcgcg cgtccccgcg agccgaaggc gcgacaacgt
     9001 ccgcggtgcc ttcgcggcct ccgcccgcgc acgcggggag agcctgctcg tcgtggacga
     9061 cgtcatgacc acaggcgcga ccgccttcga ggcggcccgc gcccttcgag gcgccggcgc
     9121 cgcggccgtc gcccttctcg tcctggcccg gactcccgag gccgggcccg cgccccgagg
     9181 atccacggag gacgcatgag ctccatcttc gaccgcctcg tcgtcggaac gctccccctg
     9241 gtgccgaagc ccgtcatctt ccgcttcgcg cgtcgctaca tcgccgggct cacgctcgac
     9301 gacgccgtcc gcacggtccg ggagctcgag gccgaggggg cgatgtcgac gatcgacgtc
     9361 ctcggcgagg ccgtcacccg ccgagagcag acggaggcga cccgggacga atacctccgc
     9421 gtgctcgacg tcctctcggc tcagcgcctc ccggcgaacg tctcggtcaa gccgacggcc
     9481 ctcggtctct cgatcgaccc gaagctcgtc cgggagaact tccgcgcgat ctgccggaag
     9541 gcggcggagt cggagacgtt cctcagaatc gacatggagg actccccgta caccgagtcg
     9601 accctcgcgc tcgccctcga gctgaaggag gagttcccga acgtcggcgt cgtcgtccag
     9661 gcctacatgc ggcgcacgct gcgcgacatg gaccggctca tcgcggcccg gatgaacgtg
     9721 aggatctgca aggggatcta cgtcgagccg cgggcgctcg cgtacaagga ccggcaggtc
     9781 gtcatcgaga gcttcgcggc gatcgtcgac aagcacctgt cggccggctg ctacgccggc
     9841 atcgcgacgc acgacgaggc ctgcgtccag cgggcgctcg cgacgatcga ccggctgaag
     9901 ctgaccccgg accgctacga gttccagatg ctcctcggcg tcgacccgct tctccggcgg
     9961 acgatcctcg cggccgggca ccggctgcgc gtctacgtgc cttacggcag ggactggtac
    10021 aagtactcgg tccgccgcct caaggagaac ccgtcgatcg ccgggcacac gatgcgctcg
    10081 atcctcggaa tcggccccgc ccagtcctga ccggccgccc cggctttccc gtccggcccc
    10141 ggcggcaccg ggccgcctgt ctgcggctcc gaaatccacg ccggagcggt tgactcttgc
    10201 ggtcccgggg gaccatgatt catcacgacg ccctgtcggg agtcggagtc gaacctgtgt
    10261 ggcgctccgc gcgacccctg tgtccggccg tattcgcagg ctgccttgcg gcactcgtcg
    10321 ccgcgggggc ggaagccgcg cccgacgcgg cgacggcgcc cctttccggc gtgctccggg
    10381 ccgacggagc gcccctcgcc ggcgtctcgc tcgtcgtccg cgggctgacc ggcgcggccg
    10441 cgtccgtcgt ccgcgtcctg aagaccgacg ccgaagggac cttctgtctc gcggatgcgc
    10501 gcccgggcgt ctactcggtc ctcgccgcgg tccccggatt ccgctcggcg acggcccagg
    10561 tcctccatcg cgcttcgggt gagagcctct cgttcgtccg actcgacctg gaccgggacc
    10621 gccgcggcgt cctgcccgcg ggtccggccg gagccctcga tccctggacg gcccgggcgg
    10681 tcctcgaagg cgacgtcctg cgcgacgaag ggcccgtcga cgcacccgcg ccgtcgaccg
    10741 ccgacgcccg cgtcccggcc gctccggcct ccgcctccgc gtcgatcccc ctcccgctcc
    10801 ggggtaccgt ccgctctctc caggggttcg gcgcggaagg aagcgagtcg ctctcccaga
    10861 cggcgctcga cgtccgaggc agcctcggca aggatgcgac ctggggcctg tcgggcgagt
    10921 tcgaccgcct gacggccgcc ggcggcgagc aggtgggacg cacgtcgcgc gtcgccctcg
    10981 acgtccttcc cgcgcggggc cacagcatcc acgtctcgag ccggaggagc gagctcccct
    11041 cgttcgacag ccccgacgcc cggctcgacg cgcactccgt cgactgggcc gccgagcccg
    11101 gccgcggctc gcgcgcttcc gtctcggccc ggttcgtttc ccatcgaaac ctcgagccgg
    11161 cgctcctccc gacgcccctc ttccgcggcg agggacgcgc gctcgcggtc gacgcccgat
    11221 accggagcga cgtcggcgag gggaggttcg tccgcctcca cgtcggctat cgctccgagc
    11281 tcgccgaggc gaccgtcgcc cacgccgcgc gcagcggtcg ggaagcgcgg gtcggcggcg
    11341 ccgccggcgt ccggctgctc gacgcgctcc tcgtggaggc cggcggaacg ggagactact
    11401 ccgacgcctc ccgcggactg acgcccgaag tcaccgtctc gctggaggcc ctcgacggcg
    11461 tgaccgtcta cggcttcgcc tcccgacggt tcgagcagcg ggacccgaac gttcccctct
    11521 acgggctcgt cggcatcgac gccgccgatc tcgtgcgggc gacccgttcg cactaccggg
    11581 ccgggacccg gctcgaatcg ggcgacggta cgacccgcct cgagctggaa acgagccgga
    11641 gagagatctc cgacgcgttc cagctgctcc tcgactccga gatgatcgcc cgggtcgacg
    11701 ccctctacct ctttcccggc gacgtcgcgg acgaggcctc cggctcgttt tccttcgtcc
    11761 tcggccccgg cctggcggcc cgtctcgcgc tcaccgcggg gcgggtccga ggcgacggcg
    11821 cgctgccggg cgcgctgcgg aacgaggccc gttaccaggt ctccagcgct cggatcgacg
    11881 tcctccccag cgggaccgcg gtctcgatcc gctaccggct gctcgagcag gagctcggac
    11941 ggaacgcctc ctacaggaac ggccgggaat ccgtcgacgt gacgatcgcc caggaggttc
    12001 ccattccgct cctccgcgcc ctggggtcac ggtggcaggc cctcttctcc atggcgctcg
    12061 agaaccgaag cgaaggcgac tctcccgttc gtccgaaccg gcagatgtcc ggtggtcttt
    12121 cgctcacgtt ttagcccgcc cgggtttttt ggatttcgat tcaacttttg cggtcacgaa
    12181 cgccgcaaat cgtcacagct gcaatctaaa ccccttccct gaagcggttt ccaccgctcc
    12241 aacgatccga attcctggca cttcgtttgc ctgtgccctg gcaggatgag gaacctgcgc
    12301 aggcggagca cgcgaatcct ccggtgggga tttctcggcg tcttcctcgc tctcctcgtg
    12361 ccgatcggct tcacggccgc ccggaagatc gcgaccacgg ggcagacggg actcctcctg
    12421 acccccgagg agagcggctt tcgcgttcgg gtccccggga ccgccgccgc ggccgggctc
    12481 ctcccgggcg acctgctcct gctcgtcgac ggagccgaag cccgggcgct gcccgacccg
    12541 gtgaggtcga tcgaaggcga cgtcgagctg accatcctgc gcgacggaca gctccttcgg
    12601 ctttcgggcc gggtcgacgc ctcgccctgg gacacccgct acttcctcct gctcctcgtc
    12661 gggaccgcgt tcgtcgtggc cgcggggctc gtcctgcgaa ccgcgcccga cgaggccgac
    12721 ccggccggag cgtttcactt cgccgggttt gccgcctgcg ccgccgcggt tctcgtcctc
    12781 acccccgccc cacccttcga cgcgcttttc cggttcggga ccctcctcga ggacctcgcg
    12841 cgggccttcc tgccggcgtt cctcctcgca ttcgtgttcc ggttccctcg ccgcgcggct
    12901 cgtgcgcccg gttggctctt cttcctcccg gccgtggccc tggccgcgct ctccgccgcg
    12961 acctacctcg acccgccggc cgcggacgcc gacgccacgg ccctcgtcct tcggctcgac
    13021 cgcgcgcagc aggcggcgat gctgctcggc gtcgtcgtcg cggtccttcg cctcgtcacg
    13081 ctcgcctcgc gccggctcga cctcctcgcc gagaagcagg tccgcttcct cctcgccggg
    13141 acggccgggg gcctcctgcc cgtcgtcctc ctcgacttcc tcccccggct cctcggcgcg
    13201 ccgatcccgg tcgtctcgag cttttcgatc gtgccgctcg tcctcgtgcc cgtcgcgttc
    13261 gtcgcggccc tgacgcgcta ccgcctctgg gacgtcgaga tcctcacgcg ggagacggtg
    13321 gccctcctcg gcgcggcgtt cctcggcgcg gggctcttct cgctcgcgca ggtggtcgac
    13381 ctcggccggg tcctcccggg gattccctac gggcgcggaa tcgtcgagac gggagccggc
    13441 ctcctgatcg ccctcacgtt cttccccgta cgccgggggc tgtcgaccgc gctcgcgcgc
    13501 gtccagtacg gcgagcggtt cggcgagcgc gaggggctcc tcgccctcgt acgcgacctc
    13561 gcccgcccga ggcgcctctc cgagatcggc cccctcctcg cggagcgcgt cacgaacggc
    13621 ctcggcgtgc cgcgcgcgac gctcctcctc gctctccccg acggacgatt cgacgcctcc
    13681 gcgctcgacg ggggcgaccc gcttcccgcg gcggagctcc cggccgacgc ggcgacgcgg
    13741 acgctgcgcc tctcgcgcct cgccttcacg gaacgcccca cggccgcagt ggcgcgcctc
    13801 cggcacgccg gcctccggac gatcgctccg ctcgccctct ccggccggct cctcggcctc
    13861 ttcgccgtcg cggaccggga cggccgcgac ccgctgtcgg ccgaggacgt cgagctgctc
    13921 gagaccgtcc tggcctcggc ggcgctcgcc gtcgaccacg cccgcctcta cgacgagctc
    13981 gaggcgcagg cggatcgcta ccggaaactg aaggagttcc acgaggacgt cgtcaccggc
    14041 tcccccgcgg cgatcgccgc cacggacgag tcgggccgca tcacgtcggc gaaccccgcg
    14101 ttcgaggccc tgttcggatc gggctccgcg gacctcgtcg ggcgaagggc cgaggaggtc
    14161 ctccccgaag ccgtcctctc ggcccacgct cccgcgcgcg tcgaggtgac ggtcggcggg
    14221 gagctgcgcg cgatcgacgt cgccgtctcg cccttcccgg gcgcgtcgga aggctccgcg
    14281 gcgcgggtgt gggtcctctc cgacgccacc gagctcgcgc gcctcgagag gagcctcgcc
    14341 gagaaggaac gcctctcggc cctctcgagc ctttccgccg gcgtcgcgca cgaggtgaac
    14401 acgccgctca ccggcgtcgc gagctacgcc cgcctcctcc tcgacgacac gcccgccgac
    14461 gacccgcgtc gccccgtcgt cgagaagatc gagcgccagg ccttccgtgc cgcgcgcctc
    14521 gtcgggagcc tcctcgacct ggcgcgcggc cgtccgcgcg acctctcgcc gctcgcaccg
    14581 cgggacctcg tcgcggaagc gtgccgcgct ctcgaggacg aggtgagagg gcgccgggtc
    14641 cgtctcgacg tcgatctccc ggagagcctg ccgaacgtcc tcggccacgc cgacgcgctc
    14701 gttcaggtcc tggtgaacct cctgaagaac gcgctcgagg cggtcgccgc cccgaaggag
    14761 gggcgccccg gcccgggctc ggtccggatc gccgccgccc ccctcgccgg aagcgtcgtc
    14821 ttcagtgtcg aggacgacgg cccgggcctc tcgcgcccgg agcaggagcg cgccttcgct
    14881 cccttccaca cgacgaaggg ggggcagggg ggcgtcgggc tcgggctcgc gatcgcggga
    14941 gatatcattc gcgctcacgg aggctctctc gcggtcgact ccactccggg ccggggcgcc
    15001 cggttcacgg tgagcctccc cgcgcagaca tgaaagtcct gatcgtcgac gacgagcccg
    15061 tcgtccgcga ggtccttcgg acggtcctct cgcgggccgg gtacgccacg agcgaggcgg
    15121 ccaccgccgc cgaggggctc gccctcttcg ccgacccggc ggtcgatctc gtcctcctcg
    15181 acctgatgct tcccgaccga cccgggcttt ctctcctccc ggagctccgc gagaagcggc
    15241 cggacgtccc ggtcgtcgtc gtcaccgcgt actcctcggt ggagagcgcc atcgtggcga
    15301 tgaaggaagg ggcgttccac tacgtcccga agccgttccg caacgaggag gtcgttcacc
    15361 tcgtggcgca ggcgctcgag aagcggaggc tcgtcgccga gaacctcgcg ctgaaggcgc
    15421 gcctcgactc cgggaccgac ttcccggaga tggtcggccg ttccgcgccg atggagaagg
    15481 tcttccagac gatccggcag gcggcgcccg cccgctcgac gatcctcgtc accggcgaga
    15541 gcggcacggg caaggagctc gtcgcgcgcg ctctccaccg cctctcccag cgcgcttccg
    15601 ggccgttcgt gacggtccac tccggcgccc ttccgccgga cctcctcgag tcgaacctgt
    15661 tcgggcacgt tcggggggcg ttcaccggag ccgtcgcgga gaagaagggg ctcttcaagg
    15721 tcgccgacgg cgggacgatc ttcttcgacg agatcgggac cgtccctctc gagacgcagg
    15781 ccaagctcct tcgcgtcctg caggaacgcg aattcctccc cgtcggcgcc gtcgagccgg
    15841 tccgctccga cgtccgcgtc gtcgccgcga cgaacgccga cctcgccagg ctcgtcgagg
    15901 aggggaagtt ccgggaggac ctctactacc ggctctgcgt catctcgatc ggcctcccgc
    15961 ctctccgcga gcgcaaggag gacgtcccgc tcctcgtcga ggcgctcgtc tcgcgggccg
    16021 cgagagagaa cgacaggccc gtcccgccgg tgacgcccgc cgcgatgaag gcgctcctcg
    16081 accacgactg gcccgggaac gtccgcgagc tggagaacgt cctcgagcgc gccgtcgtcc
    16141 tcgggggcgg gatcatcgac ctcgaccagc tccccgaggc ggtccggcgc tcggtcccgc
    16201 gtccggaggc cctcccgacc gacggcgtcg ccttctacga cgccgtaacc cgctacgaga
    16261 aggcgctcct ctcttcggcg atgcagcgcg ccgggggagt gcagaaggag gcggcccggc
    16321 tcctcgagct ctccccgacg acgctcaacg agatgctgaa gcgccacggg atgacctccc
    16381 ggcgcccttc gcgcgccacg gagccggagc cggcgtgagg ctccctctcg cccctctcct
    16441 cgcgctcctt ctcgccctcc cgaccgccgc cgccggagac gacgacccgg tcttctccag
    16501 tcggggagtc ccctccgggc gagtcctcgc cgagtcggag cgcgacccgt cggtcccccc
    16561 gccggcggtc ggcctcggac accggatcgg cgacgcgctg acaccgcacg acgctctcgt
    16621 ccgctacgtc cgcctccttt cgacggcctc gcgccgcgtc tccacgatgg tctacggcac
    16681 gactcccgag gggcgcgagc tcctccgcgt cgcggtctcg agccccgaga acctcgcccg
    16741 cctcgacgcg atctcggcgg acctcgcccg gctcgcagac ccggcgcgga cctcgaagga
    16801 agaggcgcgg gccatcgccg aacggagccc ggccgtcgcc tggatcgcct gcggcatcca
    16861 cggaaacgag gtcgcgggtc cggaggcgtg cgccgcgctc ctctacacgc tggccgcctc
    16921 gcgcgaggcc gacgtccaga agctcctctc ggccctcgtt gtcgtcctcg acccgtgcgc
    16981 caaccccgac ggtcacgagc gccacgtctc ctggtggcgc tccgtcgcgg gcgccgagcc
    17041 cgaccccgat ccccgggccc tcgagaacga gcccccctgg ccctccggcc gcggcaacca
    17101 cttcggcgtc gacctgaacc gcgactgggc ctgggggacg cagcccgaga cgcgcgcgcg
    17161 gatcgccgcg ctccgcgccc ttccgccgca gctctacgtg gacgtccacg agatggggcc
    17221 ggagcagagc tacttcttcc cgccgcccgc cgatccggtc cacccgcgcg ttcccgactc
    17281 cacccgcgcg tggatcgagg ccttcggcgc cgcgaacgcg gaggcgttcg acgagagggg
    17341 atggggctat ttcagccggg aggtcttcga cctcttctac cccgcctacg gcgactcctg
    17401 gcccgtgttc cggggaatgg tcgggatgac gttcgaggtc ggcgggaagg ggggcctcgc
    17461 ccaccgccgg cgcgatggcg ccgtcgtgac gctgaaggag cgcgccctga agcactggac
    17521 ggccctcggg gcgacgctcc ggaccgccgc ggccggccgc gcccgcatcc tcgccgacta
    17581 cgcggagttc tttcgctcct cggtcgcgga ggagcgtcgc gcgttcgtcg tcccgggcgg
    17641 ccaggacccg tcccgcctgt cggagctcgc ctctctcctc gccctccagg acgtcgaggt
    17701 gaaggtgacg acccgcgccc tgccgaacgt ccccgcgaag ggcgagacgc ttccctccgg
    17761 ctcgctcgtc gtcgagacgg cgcagcgctt cggccgattc gcccaggtcc tcctcgagtc
    17821 gcaggccgcg ctggccccgc gcttcctcga ggaggagcgc cgccgcctgc tgtccgagga
    17881 ggacgagcgg ttcttcgacg tgaccgcgtg gagcctcccc atcgccttcg gcctcgaggc
    17941 ccggctcctc cccggcgtcg gaccgctcgc gaacgccacg gctccctgga ggccggcgga
    18001 gcccgcttct ccgagaggag gccgcttcgg ctggctcctc cccggcgacg acgcgacgag
    18061 ccggcgctgc gcggcgatgc tcgccgccgc cggcgtgagg gtctccgcga cgacgaagga
    18121 agcccgcgcg gcctcgggtc ccgtccccgc cggctcctac ctcgtcgcgc gcgaggagaa
    18181 ccgggacgtc cccgggctcg acgacctcgt cgccggcgtc gtcggcgcgg cgaaggcgag
    18241 gccgcgccct ctcgagaccg ccgccacgct gtccggcccg tcgctcggct cctcgtcgct
    18301 cctcaggctg aagtcgcccc gcgtcgccct cctctccggg gacggcgccg accccgcggg
    18361 catcgcgttc ctccgccacg cactggagga gcgcctcggc gtccggccga cgctccggcg
    18421 ccccgagtcg gtcggctccg agggtttcga cggattcacc gcgctcgtcg tcccgcacgg
    18481 aaacgcgcgg ttccgccggt cgctcctcga agaggggacg gccgaggcga tccgacgctt
    18541 cgtcgacggg ggcggcgtcc tcgtggcgat tcgcgggggc gccacggcgc tccgggaggc
    18601 gccgctcgcc ctctcgaagg tcgcgcgctg ggagccgcct tcgacggagc ccgaggcccc
    18661 cgccgatacg ccggagggcg ccgccgccga ggccccgccg cccgcgaagc cctccgagcc
    18721 gcccccgccg acgcccgagg cggccctgga gcgggacctc gagcggcgcc ggctcccggt
    18781 cccgggagcc gcgctgaaga cccaggcgct gcccggccac ccgttcctct tcggcctcgc
    18841 cgccgccccc gcgttcctcg tcctcgacgg cgagcccccg ctccggcttc cggacgccct
    18901 tgcgaacgtc gtgacgatcg cccccgacga acccctcctc tcgggcttcg gctggaagga
    18961 ggcgctcgag cgctggaagg gcgcggcggt cgtccaggtc gaggaggcgg gacgcggccg
    19021 ggtcgtcagc ttcggcgccg accccgtctt ccgcggcgtc tggcgcggct ccgagagcgt
    19081 cttcctgaac gccgtcctcc tcggcccgtc gctcggcgcg aagggctggt aggaccgcta
    19141 ccggcgccgg aggaggacga ccgtccggtc gtcgtggaac gggacccccg cagcgaacgc
    19201 ggcgagccgc tctcgaagct cctcttcgac cgcctcgagc gggcgatcct tgcaggaccg
    19261 cgccgcctcg acgagccgct ccaggccgaa ctcctcgtcc tcggggttcg ccgcctcggt
    19321 caggccgtcg gtgtagagga ggagcaggtc cccgggggcg agagtcagcg cccccgagcc
    19381 gtagctccgc cccgcgaaga ggccgagcgg cggaccctgc gcggggagca gctcgtgccc
    19441 tcccccggcg cgcaggacga tcccggggtt gtggccggcg ttcgagtaga cgatcgatcc
    19501 cgtctcgggg tcgtagagcg cggcgaagaa cgtcacgaac ttctccgggc tcgtccttct
    19561 gtagacgagg tcgttcagcc gggcgcagag gcggtcgggc gggaagtgct ccgccgccca
    19621 ggcctggaac gccgactgga ccgacgccgc gaggatcgag gcggccatcc ccttcccgca
    19681 gacgtccgcc acgacgacgt cgaccgtccc gtcgccgcgg acgaagaagt cgtagtagtc
    19741 gcccgagacc gtccgagacg ggagcgtccg gccgaggagc tccgaggacg gcagctccgg
    19801 cggcacctcg gggaagaggc ggcgctgcac gtcccaggcg agggccatct ccctttcgat
    19861 ccgctgcttg gcggccgccg tctcctgcag gcgctggttc tggatcttga tcgccgccgt
    19921 gttcgcgagc gacgtgagga ggcggaggtc ctcctccgtg aagctcttcc gcccgaggcg
    19981 ggcctcgagg tagacgaggc cgaggaccgt ctccccggcg aggagagggg cagcgaggca
    20041 ggacgtgatc ccctggagcc ggatcgagtc ggccgtcccg agcttcgcgt cggtcctcgt
    20101 gtcgatcatc aggacgccct cgcggcgctc cgtgaccgcc tcgacgaggg tccgcgacag
    20161 gcggagatcg gcggggtcga ttccctcggc gagcttcagc gcctcgggct gaaggctccc
    20221 cgcctcgtcc cgcagcatca ggagcccgcg atccggctcg aggtacgcga agagcttctc
    20281 caggatgagc gacgagaggt ccgcgagcgg gatgtcggtg agcagggcga gggagacctc
    20341 gttgaggatc tggagcgacg tggcgagccg cttcagttcg gccgcgtcgg tcagctcacg
    20401 cgcggccgag cgggccagcc cggccgcggc gctcgcggtg gagatcatcg tcttctcgat
    20461 cggtccgccg cccgcctcga tgacgacctg cgccgacgac gacgcctcct cggagacctc
    20521 gatcgtcgtc tcgccgagga cgatccggtc cccggcccgg acggggacgg gctccgcgat
    20581 ccgggcccca ttcagctgag tcccgttccg ggagccgagg tcgacgagga cgaccccgcc
    20641 cggaccccgg gtcaggcggg cgtgcgcgcg agacagcgtc cggtcgggga ggaagacgtc
    20701 gttcgtcgag gcccgaccga gcgtcagctc cggtcggtcg acgcggaggc ggcggggggg
    20761 ctcgccgggg ctgtgtatcg tcaggaccag cacgggaact cagtttatcc ccgggcccct
    20821 ccgtcccttc ccgtcgcgcg gccgtcccgt tcccggggcc ggcgacgccg ccggttgtgg
    20881 tatcttcccc ggttccggcc cggctcacat gcggccgggc gacgaaggag cctgcccgtg
    20941 aaggaaaaga tccaccccac gtaccaggag gtcaccgtcc actgttcctg cggcgcgacc
    21001 tggaagaccg ggagcaccat gaaggagctg cggctggaga tctgctcgaa ctgccacccc
    21061 ttcttcaccg gcaaggccaa gctgatcgac ac
//
LOCUS       DAOTEZ010000017        20613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_1707_length_20613_cov_6.077731, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000017 DAOTEZ010000000
VERSION     DAOTEZ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 20613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20613
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1707_length_20613_cov_6.077731"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="P5164_01640"
     CDS             complement(<1..505)
                     /locus_tag="P5164_01640"
                     /inference="COORDINATES: protein motif:HMM:NF020076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HRY42623.1"
                     /translation="MKRAALLALLAALPAAAQTGLLVPTSTGRPDARVLSLREMAVDV
                     GVARGYARVNVRQVFENHTGDVQEGTWRFRLSPSGAVGDFAVWDGLVRIPGVIVEKKR
                     ARALYEELTTRRIDPGLLQQGEEEDASSGGPGRPSGGAVFSVKVSPIPAWGTKRLELQ
                     YQEEVPWA"
     gene            complement(502..2352)
                     /locus_tag="P5164_01645"
     CDS             complement(502..2352)
                     /locus_tag="P5164_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42624.1"
                     /translation="MSPAARRALLFAGGAFLAAGAAALLLLRRIDRAVAGAAAPAAVR
                     AESVRTLGFEAASRSRAAGDVAGDGAVLDVAEVSGDLLLGTGSGLVAADRTLGIRDGL
                     PSLRVGAVASWRGTPVFALERGGWGRLGPSGPERAASGFDPLEVRDFAETEGGELLVG
                     ARQGLFRAPFGAGEIERLDGAPVLSLAVLPSGEVAAGGETGLRVVAPGRAEARLVATP
                     DRWVSRVAVVGETLWVATATGAAHGPLDGTTLTAHPRGGDAVDGAGFDGAFHFVPADA
                     PPRLASLLPDGTRREVPAPERLRRLFVAGGRLLADGSAGLRRYDGARGFELVREAAPG
                     LPAAHVNALAADGTRLWLGFFDGGLATADPRATPLQARPAPSGGAWGVNAILPAAGVV
                     WAATLRGAFRIEGDDVRAIDGAGAAFALAQTTSGLAIGYGQGVLLPARRLLSAWHGLP
                     GNQAYALAPDGASLWVGTPTGLGRIERQRVSGRVLPGEGKLPHPWVTALLVGEDGLWI
                     ATYGGGIAVRRRGGEEAPFEPFPETRGLKVNAGALLALPDGRLCAGTQGSGVWCSDRT
                     RTRFARVDLPLPSPDVFSLAAFPKEAPEHLFAGTSEGLLRLPADALPETR"
     gene            complement(2405..3856)
                     /locus_tag="P5164_01650"
     CDS             complement(2405..3856)
                     /locus_tag="P5164_01650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HRY42625.1"
                     /translation="MDLTELLKFVTKKEASDLHLKPGRPPLLRINGRLVPLKTDPLQP
                     KEIEDMVLTILTPHQKEKLTANYAVDIGYGIQGLARFRGNIFLQRGSWSAVFRRIPFT
                     IPGVEELHLPDVIQTFCDYPSGLVLVTGPTGSGKSTTLAGIIKGITERHPVHVVTVED
                     PIEYLFQDNLGSVCQREVGTDTVSFHEALRNAMRQDPDVIMVGEMRDWETMQTAITAA
                     ETGHLVFSTLHTNNAAQTIDRILDSCPGGQQNQVRRQLALVLRSICSMQLIERIDGQG
                     RVPVIEILVNSPKITKLIEEGHSNEILDEMESSVTYYRMQSMNQSLLALLVNGAIDYE
                     KAMQLTADPEDLSLKLRKLFPQIEEKFRGGIMAPSANDFSAITELMDIKKLYEEAEEK
                     WKLRLQEKDDLIANLEADLQSKDRVLEANTMGSQELESELQKARQENERVRQEAAARI
                     AQLNERIKELNNRIMGGANPPSQGTPQSGFFKR"
     gene            complement(3879..5132)
                     /locus_tag="P5164_01655"
     CDS             complement(3879..5132)
                     /locus_tag="P5164_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42626.1"
                     /translation="MSPLPEPRAALARLARRSAARLARRASDTSGVPFLLVGGAVRDA
                     LLGLPSGDLDLAVPRAGAGAFAAALARLAGTRVAQIGRAPRRILHVPLRRSSVDVWET
                     DGDPAADLLRRDFTVNALALAFPGARLVGPAGALDDLAGRRLRLPRAGVLLEDPLRVV
                     RAARFLARLPGFRLDPGMRPELAAAARDLEAVAPERRLAELDAILAAGPARAARALRR
                     LEAWGGLAALLPGTDAAERRRGLAALGAVRRDAPSALLRAVLLSGAPPDRAAAALDAL
                     RASRNDRRLAAALAALPAPPARPDTTEAIRVLRRAAPFSREAIAFVEATGGRNGRALA
                     RLSDAVLTAPGALARLLHPRRPLAVHEVATILGAAGAELGRALERLDDALATGAVRGA
                     GPARAFLSEGSPPRPPRGRRERLPV"
     gene            5196..5489
                     /gene="gatC"
                     /locus_tag="P5164_01660"
     CDS             5196..5489
                     /gene="gatC"
                     /locus_tag="P5164_01660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRY42627.1"
                     /translation="MRIDGGLVRRMARLAAMDVTDGEAARLAEELSRVLDHFDAIRAV
                     PDDLLPPVPDAPPAPLRDDVPSSGPGERAYVAANAPERARGHFVVPRVVSRNG"
     gene            5486..6955
                     /gene="gatA"
                     /locus_tag="P5164_01665"
     CDS             5486..6955
                     /gene="gatA"
                     /locus_tag="P5164_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRY42628.1"
                     /translation="MTGDLARLLDGPAHGLAAAVSAREVRARDVAGAALLRAGRSRER
                     HGAFLHVAGEDAIEAAAEVDRRVAAGEALPLAGVPIAVKDNVHVAGMPTTAGSRILGA
                     FVPPEDATCVARLKAAGCVVVGKTSCDELGMGSTNETSAFGPVRNPWEPTRVPGGSSG
                     GSAAAVAARAVPLAVGTDTGGSVRLPAAFCGLVGVRPTFGRVSRLGLVAFASSFDQAG
                     PLARHARDAALALEAMSGADPRDATSLPGASPDLLSRLDDGVAGLRVGLLDDEEGVHP
                     DAAAALSATARLLASRGASLVPLRLPRLALGVAAYYLIAAAEASSNLARFDGVRFGAR
                     AAGDGPAELVRATRAAGFGDEVKRRVLLGTFALSAGHREAWYERAQRVRTRIALDLGE
                     AFRACDLLLSPTAPGPAFPIGEKSGDPLSLYLTDVFTVPASLAGLPAVAVPAGLSRER
                     LPVGVQLAGPAGSEALLLAVARELEAARGPWTAPPDVIR"
     gene            6952..>8815
                     /locus_tag="P5164_01670"
     CDS             6952..>8815
                     /locus_tag="P5164_01670"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRY42629.1"
                     /translation="MRRALSTGALALLLLVPLIAPAQTAPRRVEVSEDFSAFVDQGAI
                     LVEVRPLDGESVTDFARRISRDEASASRLLALPAAPSRSRRIVLPYGDLSDEARLAAV
                     RALFPSDVRATIGWLHVAVETEPLASIATWFTGDARLASRLADLNGLREGEPATRGTL
                     VKVPVEYLLPPFRDAEPIPDEEPPRLEYGSDAKGRYAVYRLKRKEALYSAVVVRFTGR
                     IHAEDVIQLALDIALRSGIEDVHAIPVGFPVKIPLELLSEEYLPPDDPKAAAQAKERA
                     EAALFATPAKARHLAGVRVVIDAGHGGTDTGTLHHGVWESTYVYDVASRLRKVLLETT
                     RAEVVMMTREPVQGWEVIDRDRLPDRKGRVLLTDPPYEMTNVVAGVNLRWYLANAILK
                     RPGAGGRPIPPEKTVFLSLHADSLHPSVRGAMAYVPGERFLRERYGRATEFYLRFREV
                     RDDPVVSFTKKERVLAEGASTSLAEKLIGAFRRRDLPVHQFRPVRTHVIRKGREWVPA
                     VLRYNKIPNRVLFELSNLSNEEDRKLTLTRANRQECAEALADGLVAFFGGAAEERVAA
                     PAAAPGRATPPAEAESAPAEVSGPWPDTVGPWPQGAPQGRTAISTASTPTPTKAP"
     assembly_gap    8816..8915
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8964..9875)
                     /locus_tag="P5164_01675"
     CDS             complement(8964..9875)
                     /locus_tag="P5164_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY42630.1"
                     /translation="MNPSPAARSRALLGVFLCVLVWGANYPATKIAYRELSPLAYTGW
                     RFLLAAALILAWARRNHAPFLPPRGLRLPGFLLAMTGVGVYQLFFAIGVAKTSGFAAA
                     LLNSVSPLLSLLLVALLGQERIPRPAVIGTVVAWGGVLAFLYSAHGSADLGGLSGNLL
                     CLVSATCWSVYSVLSSRYSTRIPHATALASTFTFGVPVLLAYCLPAMLRQDYSDVSAT
                     AWTILVLSAVFPLYVSFRLWAHGLAVLGVAATTRVGVLVPLVAGAASALWTGERFSGG
                     KVVSAAVVLGGLALARLGTPRELPAKA"
     gene            9971..10975
                     /locus_tag="P5164_01680"
     CDS             9971..10975
                     /locus_tag="P5164_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012462749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY42631.1"
                     /translation="MLEVDALRKSFYGTLAVDDVSFRVESGEILGFLGPNGAGKTTTM
                     RMITGFLEPNAGRVTVDGIDAAQKPREARARIGYLPETLALYPEMRVREYVKFRAELC
                     GVPRRETKARVEDALATCFVDDVASKPIGNLSKGYRQRVGLAAALVHAPQVLVLDEPT
                     VGLDPRQIVKVRDLIRDLKKDHTILLSTHILPEVELVCDRVVIIDRGKIVATGTPETL
                     RQQLTQAPGLAVAIQGEVPDADGILRRLPGVVRAARIGAGGETRVLLEAERGADLREA
                     VFRECVARGWTLLELAPRQATLEDVFVRLLATRDAEEGPGGEGLPAAVEEPGAGEAKP
                     "
     gene            10972..11709
                     /locus_tag="P5164_01685"
     CDS             10972..11709
                     /locus_tag="P5164_01685"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY42632.1"
                     /translation="MKALFAILKREFLAYFFSPLAYVVLTAFLFINGYVFYAILLSLN
                     SPETPRQELMALFFKNVFYWIFMMLVSSIVAMRLVSEERKSGSIEALLTAPVGEGTVI
                     AGKFLGGLTFFLFLWLPTVLYPVALSRYGRIDFGPIASSYLGIALFGALFIAAGTFAS
                     ALTKNQIIAAIVSFVMILALFLAGVFRELVTDPKLREGLGYLNLIEHMDDFARGLVDT
                     RRVVYVVSAVVFFLFLATKALQGNKGK"
     gene            11711..13150
                     /locus_tag="P5164_01690"
     CDS             11711..13150
                     /locus_tag="P5164_01690"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HRY42633.1"
                     /translation="MSESRTARRVNLGLAILVGLGILALVNWLGARHYRRFDWTSAGL
                     YTLSPKSAEVMKALTKPVSATVFLTEGSPLYAETQELLKRYQAASPLLTVETLNPERN
                     PARAQAFLKEMGDIRLSVVFRSGEKKKVVPVDALADWDFSRAEMGGEPALKGFKGEQE
                     FTAAILSVTQERSPKVVFTTGHGERPLSGRLSEGYERAADLLKQDNCTVEEWDSLADQ
                     KVPDGTDLVVLAGPRAAFTFPETDALLAFLRNGGRALLLLDPEFAPGAGNRLVDLGLG
                     PVLEAYGIRLGDDIVVDPRNALPLMGPETVVSRSFRSHPVTKLLQGLPVVFPVIRSVG
                     AVEPAPKDVSVTVLAETSAEGWGETNLADLETRVAKDEKDVAGPVPLAVAAESATEKK
                     ARLVVVGDADFASSGGIANAANLYLLSSAVNWLLERESLISIPPKSTDQVSVSLSRGD
                     VGRITLLVLLVLPIAAIALGLAVWLRRRR"
     gene            13161..14546
                     /locus_tag="P5164_01695"
     CDS             13161..14546
                     /locus_tag="P5164_01695"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HRY42634.1"
                     /translation="MSTRKLLILTGVFFALLAFVVLWERHQPTSDEAAKARKRLVDLD
                     PKEVSRLTLERAEQAKVELVREDGRWTLAGPKGGAADAVTADGLAADLARLDLLGETR
                     TEFDPKAHGLASPRVRVTVTTTDGKARTILFGDPVPGADATAASADGRLGAVRFAPIA
                     QLTKPYDEYRSRSLLDVPTAEIDRVTVVRGPNRVVAVRDGAGWLLEQPVADLASGSFV
                     DQLLADLAGVRATEFPTVAAADMPRIGLSPPAAEVLLGKGKEVLARLAFGAAKADAAG
                     KLYAARDGVVMVVDDRAQETLGKELSAFREGRLLPLDSWLVTRVVFESEALRAGAEKL
                     EGAWRSAGREVDGRVPEDLLERLGRAEVRRFVAAKDLASIGLAPVRRRSPVPAGSVEV
                     LLEKAKKPVRIVFFSTPSLAAEQLVAARVDGRGDTLVVDASVWDDVRSFAVRLKEAAA
                     AGPTPTAAPRP"
     gene            14675..15769
                     /locus_tag="P5164_01700"
     CDS             14675..15769
                     /locus_tag="P5164_01700"
                     /inference="COORDINATES: protein motif:HMM:NF039197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5694 domain-containing protein"
                     /protein_id="HRY42635.1"
                     /translation="MPERPTDPRCAAPPPSRPRTAVLAAVLGTALAAFPCAAGEVMVV
                     GTPHLATLRPEPGPERLREVVERLASWAPTVVCIEAIPGDRVRQLAGDPAAWGELLST
                     FAVDAIRLAPEQQARLSVDGATALRLARELEARPGSLTDAERVRLVGLQLAGHEPWSA
                     LLGWTVLTDEERAAAAKSLGRAAVERLGALASSRNEIVSLAVPLAARLGHRRLCMADV
                     FVDEVAVQTLEEDLVPLFRDPGVAKGLEAIEAAKAARWKPEEKDGLVRLVAWMSASDY
                     AEADRRTQWDLFARAGVPNRAGERRLALWHARNAEIAAHVYRAASGPDGARTLLLIGA
                     AHRPFLEAALRAQPWIDVVPAERALGVVGR"
     gene            complement(15766..17244)
                     /locus_tag="P5164_01705"
     CDS             complement(15766..17244)
                     /locus_tag="P5164_01705"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRY42636.1"
                     /translation="MASSEHLEALKGPDWNQWRTEHSGITPDLTDVSLGGLDLSGKNL
                     AGADLSRSKLDGAKLDGANLSGAHLQGTSLRGTSLQRTDLSNTDLEYQTLDKVSLVGA
                     ALRGVNLKNASVREANLTACDLSTAKLEGTTFSGSVFSYSRLVAANFRGVVIDGCVFE
                     ECDLSQAVFDGITYQRGRFDKSTLTLAFFRDAKLAGTSFRGVKGQRITFRGANLTGVD
                     FTGADLTSSDFERVIAQAADFSDANLRKACFKDADLRRSILLGALLEETDLTQANLEK
                     LDLRTPKLKFPNFTGAKLAEANGVGANLRGGNYTDADLSRADLTEANLEGGIFQRAHL
                     EGATLRRANLKRARMAGVLAPGADFSGTNLLGAEAQEADFSGADFRFSTLVGAVLEGA
                     LFKGANVAHADFSEAKLGGSSFVGAKGEGAKFTGQLHLKLVLAAGAFLGSSKKKVEKV
                     EDRKEQERKARVARLEREAAERDRSVASRPARGETDPPERKS"
     gene            17276..18778
                     /locus_tag="P5164_01710"
     CDS             17276..18778
                     /locus_tag="P5164_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY42637.1"
                     /translation="MSAVVPVRGAERIRRALLGSYPLVYVQSWEEGRVERAVAMLAQK
                     FYERPVPFAVWTCVEGWTGLPEAPTETRDPVAALEAVLRANGPGFFLMKDLPALFDGR
                     ADVVRKLRDVYRQAKGKGKFVVLVSPRLILPEDLKKEVFLVDYDLPDEAEVRNAISVL
                     GRRYLGEAGLSPADADRVVTVLRGLTLDELEHVFSKVFARTPRFDEATFDELLAEKEQ
                     SSRKEGVLEYVPPRFTLDDVGGLENLKDWLRKRQHLFTREAVEAGLPVPKGLLMMGMS
                     GCGKSLSVKTIPALWNLPLFRLDMNLVFGTANPEATFHRALKTVEGLAPSILWIDEIE
                     MAITGGREAGGGDPALGRIFSTFLTWMQEKEALVFVAATANRIHLLPAEFIRKGRFDQ
                     VFFLDLPNETERKAIFTVHLKKAKADLSKFDVVFLAKATKGFNGAEIEAVVQASAIDA
                     FNEKRPLDEDAVSRVITNTVPLSRTMDEQIKAIKSWAHNRALSASRESAS"
     gene            complement(18815..19474)
                     /locus_tag="P5164_01715"
     CDS             complement(18815..19474)
                     /locus_tag="P5164_01715"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HRY42638.1"
                     /translation="MSRLLPLFPLDAVLLPQTRLPFHVFEPRYRAMLADALDGERLLG
                     LHTLAPDAPPLPDGRPALLPYGCAGEIVEHEPLPDGRSNIVLLGLFRYRIVAERDGRP
                     YRLADVEEAPVAPLAPGRAGGPGRRELRRLLARDVERLADSVGRSEARHLPPSLSDEG
                     LVNEAASRLGLGPEERYALLAMQTLESRYAWVLDQVRSLQSRIDLLAPYRREGDDPRR
                     N"
     gene            complement(19471..19830)
                     /locus_tag="P5164_01720"
     CDS             complement(19471..19830)
                     /locus_tag="P5164_01720"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRY42639.1"
                     /translation="MRASLRIMIAVALIAAGASSGCTADPDPAAGVWKRRCAACHGSD
                     GAGRTRFAEGRPYADLTDGKWKHGPEREALRRLVAEGDPTSTMPPFAGTLSPEEIDAV
                     VEWALALPARTEGGSAR"
     gene            19847..20080
                     /locus_tag="P5164_01725"
     CDS             19847..20080
                     /locus_tag="P5164_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HRY42640.1"
                     /translation="MANKDDRYTDNASGKFYVDTQCIDCDVCRVTAPNNFARQEEKGY
                     SYVTKQPDTPEEEAQCQEAMDSCPVEAIGNDGE"
     gene            complement(20094..>20613)
                     /locus_tag="P5164_01730"
     CDS             complement(20094..>20613)
                     /locus_tag="P5164_01730"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686416.1"
                     /note="catalyzes the radical-mediated insertion of two
                     sulfur atoms into an acyl carrier protein (ACP) bound to
                     an octanoyl group to produce a lipoyl group; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRY42641.1"
                     /translation="TTPGVTVEVLIPDFLGNEEALRTVLEAAPEVLNHNTETVARLYR
                     RVRPDAIYARSLELLSRAAAWRDGTAPAMRVKSGLMVGLGETVDEVLETMRDLRAAGT
                     DTLTVGQYLQPHARRLPVERWWTPEEFARLRDEGRAMGFAVVEAGPLVRSSYHARAAL
                     GAAGGGGAAAPR"
BASE COUNT         2739 a         7369 c         7628 g         2777 t
ORIGIN      
        1 cggcccacgg gacctcttcc tggtactgga gctcgagccg cttcgtcccc caggcgggga
       61 tcggcgagac cttcacggag aagacggctc ctcccgaggg gcgcccggga ccgccggagc
      121 tcgcgtcctc ctcctcgccc tgctgcagca gccccgggtc gatccggcgc gtcgtgagct
      181 cctcgtagag ggcgcgtgcc cgcttcttct cgacgatgac gccggggatc cggacgaggc
      241 cgtcccagac cgcgaagtcg ccgacggcgc ccgacggcga gagccggaag cgccaggtcc
      301 cttcctgcac gtcgccagtg tggttctcga agacctgccg gacgttcacg cgggcgtagc
      361 cgcgcgcgac tccgacgtcg acggccatct cccggagcga gaggacccgg gcgtccggac
      421 ggcccgtcga ggtcggcacg aggaggccgg tctgggcggc ggccgggagc gcggcgagga
      481 gggcgaggag cgcggctcgt ttcatcgtgt ctccggaagg gcgtcggccg ggaggcggag
      541 gagaccctcc gacgtgccgg cgaagaggtg ctcgggagct tccttcggga acgcggcgag
      601 ggagaagacg tcgggagagg gaagggggag gtcgacgcgc gcgaagcgcg tccgcgtccg
      661 gtcggagcac cagacgccgg agccctgggt gcccgcgcag agccggccgt cggggagcgc
      721 gaggagcgcg ccggcgttca ccttcaggcc ccgcgtctcg gggaacggct cgaaaggggc
      781 ttcctcgccg ccccggcggc ggacggcgat tccgccgccg taggtcgcga tccagaggcc
      841 gtcttcgccg acgaggagcg ccgtcaccca cggatggggg agcttcccct cgcctgggag
      901 gacgcggccc gacacgcgct gccgctcgat ccggccgagg ccggtcggcg tccccaccca
      961 gagcgacgcg ccgtccggcg cgagggcgta ggcctggttc cccgggaggc cgtgccaggc
     1021 cgagaggagg cggcgtgccg gcaggaggac tccctgcccg tagccgatcg cgaggccgga
     1081 cgtcgtctgc gcgagcgcga acgcggcgcc ggccccgtcg atcgcgcgga cgtcgtcgcc
     1141 ttcgatacgg aaggccccgc gaagcgtcgc cgcccagacg acgccggccg cgggcaggat
     1201 cgcgttgacg ccccacgcgc cgcccgaggg cgccggccgc gcctgcagag gcgtggcacg
     1261 cggatcggcc gtcgcgaggc ccccgtcgaa gaaaccgagc cagaggcgcg tgccgtcggc
     1321 ggcgagggcg ttcacgtggg ccgcggggag cccgggcgcc gcctcgcgga cgagctcgaa
     1381 tccgcgcgcc ccgtcgtatc gccggagacc ggccgatccg tcggcgagga gccggccccc
     1441 ggcgacgaag aggcgccgga ggcgctccgg agcgggcacc tcgcggcggg tcccgtcggg
     1501 gaggagcgag gcgaggcggg gcggggcgtc ggcggggacg aaatggaacg cgccgtcgaa
     1561 cccggccccg tcgacggcgt cccctccgcg tgggtgggcc gtcagagtcg tcccgtcgag
     1621 cggcccgtgc gccgccccgg tcgccgtggc gacccagagc gtctcgccga cgaccgccac
     1681 gcgcgagacc cagcggtcgg gcgtcgcgac gaggcgcgcc tccgcgcggc ccggcgcgac
     1741 gacccggagg ccggtttcgc ccccggcggc gacctcgccc gacgggagga cggcgagcga
     1801 gaggaccggc gcgccgtcga gcctctcgat ctcgcccgcg ccgaaggggg cgcggaagag
     1861 cccctggcgg gcgccgacga gaagctctcc tccctcggtc tccgcgaagt cgcgcacctc
     1921 gagagggtcg aaccccgagg ccgcacgctc cggacccgaa ggtccgagcc ggccccatcc
     1981 gccgcgctcg agggcgaaga cgggcgtccc gcgccacgac gccacggcgc cgacgcgcag
     2041 ggaagggagg ccgtcgcgga tcccgagcgt ccggtcggcg gccacgagcc cggagccggt
     2101 gccgagcagg aggtcgccgg agacttccgc cacgtcgagg accgctccgt ccccggcgac
     2161 gtcgcccgcg gcacgcgacc gcgacgccgc ctcgaacccg agagtgcgga cgctctcggc
     2221 gcggaccgcg gcgggcgccg ccgcgcccgc cacggcgcgg tcgatccggc ggaggaggag
     2281 cagggcggcc gcaccggcgg cgaggaaggc tccgcccgcg aagaggagcg cgcggcgcgc
     2341 ggctggggac atgctggtcc gagtctaacg ggcgccggag ccggcgcggg agccttccgt
     2401 ccgcctagcg cttgaagaag cccgactgcg gcgtcccctg agaaggcggg ttggcgccgc
     2461 ccatgatccg gttgttgagc tccttgatcc gctcgttgag ctgcgcgatc cgcgcggcgg
     2521 cctcctgacg gacccgctcg ttctcctgcc gcgctttctg cagctcgctc tcgagctcct
     2581 ggctccccat cgtgttcgct tcgaggacgc ggtccttgga ctggaggtcg gcctcgaggt
     2641 tcgcgatcag gtcgtccttc tcctggaggc ggagcttcca cttctcctcc gcttcctcgt
     2701 agagcttctt gatgtccatg agctcggtga tggcggagaa gtcgttcgcg gacggggcca
     2761 tgattcctcc tcggaacttt tcctcgatct gggggaagag cttgcggagc ttcagggaga
     2821 ggtcctcggg gtccgccgtg agctgcatcg ccttctcgta gtcgatcgcc ccgttcacga
     2881 ggagcgcgag aagcgactgg ttcatcgact gcatccggta gtaggtgacc gacgactcca
     2941 tctcgtcgag gatctcgttg gagtgcccct cctcgatcag cttcgtgatc ttcggagagt
     3001 tcacgaggat ctcgatgacc gggacgcgcc cctgcccgtc gatcctctcg atcagctgca
     3061 tggagcagat cgaccgcagg acgagggcga gctggcggcg gacctggttc tgctggccgc
     3121 ccgggcagct gtcgaggatg cggtcgatcg tctgcgccgc gttgttcgtg tggagcgtgg
     3181 agaagacgag gtgcccggtc tcggcggcgg tgatcgccgt ctgcatcgtc tcccagtcgc
     3241 gcatctcgcc gaccatgatg acgtccgggt cctggcgcat cgcgttgcgc agcgcctcgt
     3301 ggaaggagac cgtgtccgtc ccgacctcgc gctggcagac ggagccgagg ttgtcttgga
     3361 agaggtactc gatcgggtcc tcgacggtga cgacgtgaac cgggtggcgc tcggtgatcc
     3421 ccttgatgat cccggcgagc gtcgtcgact tgcccgagcc ggtcggcccg gtgacgagga
     3481 cgaggcccga agggtagtcg cagaacgtct ggatgacgtc cggcaggtgg agctcctcga
     3541 cgcccgggat cgtgaacggg atccgccgga agacggcgga ccagctcccg cgctgcagga
     3601 agatgttgcc gcggaagcgg gcgagcccct ggatgccgta gccgatgtcg acggcgtagt
     3661 tcgccgtcag cttctctttc tggtgcggcg tgaggatcgt gaggaccatg tcctcgatct
     3721 ctttgggctg gagcgggtcg gtcttgagcg gcacgagccg cccgttgatc ctgaggagcg
     3781 gggggcggcc cggcttcagg tggaggtccg acgcctcctt cttcgtcacg aacttcaaca
     3841 gttcggtcag gtccatgttc cagatcctcg cacgaagctt ataccgggag gcgctcccgc
     3901 cgccctcgtg gcggacgggg cggggaccct tcggagagga acgcccgggc ggggccggcg
     3961 ccgcggacgg ctccggtcgc gagggcgtcg tcgaggcgct cgagggcgcg accgagctcc
     4021 gctcccgccg cgccgaggat cgtggcgacc tcgtggacgg cgaggggccg ccgggggtgc
     4081 aggagccggg cgagcgcgcc gggtgcggtg aggacggcgt cggagaggcg cgcgagggcc
     4141 cgtccgttcc ggccgccggt cgcttccacg aacgcgatcg cctcgcgcga gaagggggcg
     4201 gcgcggcgaa ggacccggat cgcctcggtc gtgtcgggcc gggcgggagg agcgggcagg
     4261 gcggcgagcg ccgccgcgag ccgccggtcg ttccggctcg cgcggagcgc gtcgagggcc
     4321 gccgccgcgc ggtccggcgg agctcccgaa aggagcaccg cccggagaag cgcggacggg
     4381 gcgtcgcgcc ggacggcccc gagcgcggcc aggccgcgac gacgctccgc ggcgtcggtc
     4441 ccaggaagga gcgcggccag gccgccccac gcctcgagcc ggcggagggc gcgcgcggcc
     4501 cgtgcggggc ccgcggcgag gatcgcgtcg agctcggcga ggcgacgctc gggcgccacg
     4561 gcctcgaggt ctcgcgcggc ggccgcgagc tcggggcgca tcccggggtc gagccggaag
     4621 ccgggaaggc gcgcgaggaa ccgggcggca cggacgacgc ggagggggtc ctcgaggagg
     4681 acgccggcgc gcgggagccg cagacgccgc ccggcgaggt cgtcgagggc tcccgcgggg
     4741 ccgacgagac gggcgcccgg gaatgcgagt gcgagcgcgt tcacggtgaa gtcccgccgg
     4801 aggaggtcgg cggcggggtc cccgtcggtc tcccagacgt cgaccgacga ccggcgcagc
     4861 gggacgtgga ggatccgcct cggcgcccgg ccgatctgcg cgacgcgcgt ccccgcgagc
     4921 cgcgcgagcg ccgcggcgaa ggcgccggcg ccggctcggg gcaccgcgag gtcgaggtcc
     4981 ccggaaggga ggccgaggag cgcgtcgcgc acggcgccgc cgacgaggag aaacgggacg
     5041 ccggatgtgt cggacgcccg ccgcgcgagg cgggccgcgc tccggcgcgc gaggcgggcc
     5101 agcgcggcgc gcggctcggg cagtgggctc acgataggcc gcgacggtag gggagcggtc
     5161 cgggaggcgt caagccgctg gctatactcg aatccgtgag gatcgacggc ggcctcgtcc
     5221 gcaggatggc gcggctcgcc gcgatggacg tcaccgacgg agaggcggcc cgcctggccg
     5281 aggagctgag ccgcgtcctc gatcatttcg acgcgatccg cgcggtcccc gacgacctcc
     5341 tgccgccggt ccccgacgcg cctccggcgc cgcttcggga cgacgtgccg tcctccgggc
     5401 cgggggagcg cgcctacgtc gccgcgaacg ccccggagag agcgcgcggg cacttcgtcg
     5461 tgccgcgcgt cgtctcgagg aacgggtgac cggggacctc gcccgtctcc tcgacggacc
     5521 ggcgcacggc ctcgccgccg ccgtctcggc ccgggaggtg cgcgcgcggg acgtcgccgg
     5581 ggccgcgctc ctccgggcgg gacggagccg cgaacgccac ggggcgttcc ttcacgtcgc
     5641 gggagaggac gcgatcgagg ccgcggccga ggtcgaccgc cgcgtcgcgg cgggcgaggc
     5701 gctgccgctg gcgggcgttc cgatcgccgt caaggacaac gtccacgtgg ccggcatgcc
     5761 gacgacggcc ggctcccgca tcctcggcgc tttcgtcccg ccggaggacg cgacgtgcgt
     5821 cgcgcggctg aaggcggccg gctgcgtcgt cgtcggaaag acgagctgcg acgagctcgg
     5881 gatgggctcc acgaacgaga cgtcggcgtt cgggccggtg cggaacccct gggagccgac
     5941 gcgcgtcccg ggcggctcct ccggcggctc cgccgccgcc gtcgcggcgc gcgcggtccc
     6001 gctcgccgtc gggaccgaca ccggcggctc cgtccgcctc ccggccgcgt tctgcgggct
     6061 cgtcggcgtg cgtccgacgt tcggccgcgt ctcccgcctc gggctcgtcg ccttcgcctc
     6121 ctccttcgac caggccgggc cgctcgcgcg gcacgcgagg gacgcggcgc tcgcgctcga
     6181 ggcgatgtcg ggcgccgacc cgcgcgacgc cacgtccctt cccggcgcgt cgcccgacct
     6241 cctctcccgg ctcgacgacg gcgtcgcggg gctccgcgtc ggcctcctcg acgacgagga
     6301 gggggtccat ccggacgcgg cggcggccct ctcggcgacg gcgcgcctcc tcgcgtctcg
     6361 cggagcttcg ctcgtcccgc tccgccttcc ccggctggcg ctcggcgtcg cggcgtacta
     6421 cctgatcgcg gccgcggagg cctcctccaa cctggcgcgg ttcgacggcg tccgcttcgg
     6481 cgcgcgcgcc gcgggagacg gcccggccga gctcgtccgc gcgacgcgcg cggccggctt
     6541 cggggacgag gtgaagcgcc gggtcctcct cgggacgttc gccctgtccg cggggcaccg
     6601 cgaagcgtgg tacgagcggg cgcagagggt ccggacgcgg atcgcgctcg acctcggcga
     6661 ggccttccgc gcctgcgacc tcctcctctc gccgaccgcg cccggcccgg ccttcccgat
     6721 cggcgagaag agcggcgacc cgctctcgct ctacctcacg gacgtcttca ccgtgcccgc
     6781 gagcctcgcg gggcttcccg ccgtcgcggt ccccgcgggg ctctcgcggg agcggctccc
     6841 cgtcggcgtc cagctcgcgg ggccggcggg ctccgaggcg ctcctgctcg ccgtcgcgcg
     6901 ggagctcgag gcggcgaggg gcccgtggac ggccccgccg gacgtgatcc ggtgagacgc
     6961 gcgctctcga cgggagccct cgcgctcctc cttctcgtcc ccctcatcgc gccggcccag
     7021 acggcgccgc gccgggtgga ggtctccgag gacttctcgg cgttcgtcga ccagggggcg
     7081 atcctggtcg aggtccgccc gctcgacggc gagtccgtga ccgacttcgc gcggcgcatc
     7141 tcccgcgacg aggcctcggc ctcgcggctg ctggcgctgc cggccgcccc gtcgcggagc
     7201 cggcggatcg tgctgccgta tggcgacctg tccgacgagg cgcgcctcgc cgccgtccgg
     7261 gccctctttc cctccgacgt gagggcgacg atcggctggc tccacgtggc cgtcgagacg
     7321 gagccgctcg cgtcgatcgc gacctggttc acgggcgacg cgcgcctcgc ctcgcgcctc
     7381 gcggatctca acgggctccg cgagggcgag ccggccacgc gcggcacgct cgtgaaggtc
     7441 ccggtcgagt acctcctccc cccgttccgg gacgccgagc cgatccccga cgaggagcct
     7501 ccccgcctcg agtacggctc cgacgcgaag gggcggtacg ccgtctatcg cctcaagcgg
     7561 aaggaggccc tctattcggc ggtcgtcgtc cgcttcacgg gccgcatcca cgccgaggac
     7621 gtcatccagc tcgcgctcga catcgccctc cggtcgggaa tcgaggacgt ccacgcgatc
     7681 cccgtcggat tcccggtgaa gatcccgctc gagctcctct ccgaggagta cctcccgccc
     7741 gacgacccga aggcggccgc gcaggcgaaa gagcgggccg aggcggccct cttcgcgact
     7801 ccggcgaagg ctcggcacct cgcgggggtc cgggtcgtaa tcgacgccgg gcacggaggg
     7861 accgacaccg ggacgctcca ccacggcgtc tgggagtcga cctacgtgta cgacgtcgcc
     7921 tcgcggctcc ggaaggtcct cctcgagacg acgcgggccg aggtcgtcat gatgacgcgc
     7981 gagcccgtgc aggggtggga ggtgatcgac cgggaccgcc tccccgaccg gaagggacgc
     8041 gtcctcctga cggacccgcc gtacgagatg acgaacgtcg tggctggcgt gaacctccgc
     8101 tggtacctcg cgaacgcgat cctgaagagg ccgggcgccg gcggacggcc gatccctccg
     8161 gagaagacgg tcttcctctc gctccacgcc gactcgctcc acccgtcggt gagaggcgcg
     8221 atggcgtacg tcccgggcga gcgcttcctg cgggagcggt acggacgggc gaccgagttc
     8281 tacctgaggt tccgcgaggt ccgcgacgac ccggtcgtct cgttcacgaa gaaggagcgc
     8341 gtcctggccg aaggggcgtc gacctccctc gccgagaagc tgatcggcgc cttccggcgc
     8401 cgggacctcc ccgtccacca gttccggccc gtgaggacgc acgtcattcg aaagggacgg
     8461 gagtgggtcc ccgcggtcct ccgatacaac aagatcccga accgcgtcct cttcgagctc
     8521 tcgaacctct cgaacgagga ggaccggaag ctgacgctga cccgggccaa ccggcaggag
     8581 tgcgccgagg cgctcgcgga cggcctcgtc gcgttcttcg gcggggcggc ggaggagcgt
     8641 gtggcggcgc cggccgccgc gcccgggcgg gcgacaccgc ccgccgaagc cgagtccgcc
     8701 ccggcggagg tctccgggcc gtggcccgac acggtcggcc cctggccgca gggggcgccc
     8761 caggggcgca cggcgatctc aacggcctcg acgccgacgc cgacgaaggc gccgannnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgccg acgaaggcgc cgagaaagcc
     8941 cgttcggcgc cggcgctgag gcctcaggcc ttcgccggga gctctcgcgg cgtcccgagg
     9001 cgggcgaggg cgaggccgcc gaggacgacg gccgccgaga cgaccttccc gcccgagaat
     9061 cgctccccgg tccagagcgc cgatgcggcc cccgcgacga gcgggacgag gactccgacg
     9121 cgcgtcgtcg cggcgacgcc gagcacggcg agaccgtggg cccagagccg gaacgagacg
     9181 tagagcggga agacggcgga aaggacgagg atcgtccagg cggtggcgga gacgtcggag
     9241 tagtcctggc ggagcatcgc ggggaggcag tacgcgagca ggacggggac gccgaacgtg
     9301 aaggtcgacg cgagcgccgt cgcgtgcggg atgcgcgtgg agtagcgcga cgagaggacg
     9361 ctgtagaccg accagcaggt ggccgagacg aggcagagaa ggttccccga aagccccccc
     9421 aggtccgcgg agccgtgggc cgagtagagg aaggcgagga cgcctcccca ggcgacgacg
     9481 gtgccgatca cggccgggcg cgggatccgc tcctgcccca ggagggcgac caggaggagg
     9541 gagaggagcg gggagacgga gttcaggagg gcggccgcga agccgctcgt cttcgccacc
     9601 ccgatcgcga agaagagctg gtagacgccg acgccggtca tcgcgagcag gaagcccggg
     9661 agccggagcc cccgcggggg aaggaacggg gcgtggttcc tgcgggccca ggccaggatc
     9721 agggcggccg ccaggaggaa gcgccagccc gtgtaggcga gcggggagag ctcgcggtag
     9781 gcgatcttcg tggcggggta gttcgccccc cagacgagga cgcacaggaa gacgccgagg
     9841 agggcccggg aacgggcggc gggggaggga ttcacgtgct cttcgctctg ctatatttct
     9901 cgcgctttcg tggcggaggg cggcgcggac ggcgccgccc tctgcccgtc cgcggaggga
     9961 ctgcacgacg atgcttgagg ttgacgcgct ccgcaagtcg ttctacggga cgctcgccgt
    10021 ggacgacgtc tcgtttcggg tcgagagcgg cgagatcctg ggcttcctcg gcccgaacgg
    10081 cgccggcaag acgacgacga tgcggatgat caccgggttc ctcgagccga acgcgggccg
    10141 ggtgacggtc gacgggatcg acgcggcgca gaagccgcgg gaggcgcgcg cccggatcgg
    10201 ctacctcccc gagacgctcg ccctctaccc cgagatgagg gtgcgggagt acgtgaagtt
    10261 ccgagccgag ctctgcggcg tgccgcggcg cgagacgaag gcgcgcgtcg aagacgccct
    10321 cgcgacctgc ttcgtcgacg acgtcgcatc gaagccgatc gggaacctct ccaagggcta
    10381 ccggcagcgc gtcggcctcg ccgccgcgct cgtgcacgcc ccgcaggtcc tcgtcctgga
    10441 cgagccgacc gtgggcctcg acccgcgcca gatcgtgaag gtccgcgacc tgatccggga
    10501 cctgaagaag gaccacacga tcctcctgtc gacgcacatc ctcccggagg tggagctcgt
    10561 ctgcgaccgc gtcgtcatca tcgaccgcgg gaagatcgtc gcgaccggga cgccggagac
    10621 gctccggcag cagctgacgc aggcgcccgg cctcgcggtc gcgatccagg gggaggtccc
    10681 tgacgccgac gggatcctgc gccggctccc gggcgtcgtc cgcgcggcgc ggatcggcgc
    10741 gggcggggag acgcgcgttc tgctcgaggc ggagcgcggc gccgacctgc gggaggccgt
    10801 cttccgcgag tgcgtcgccc gcggctggac gctcctcgag ctcgcgcccc gccaggcgac
    10861 gctcgaggac gtcttcgtcc gtctcctggc gacgcgggac gcggaggagg ggccgggggg
    10921 cgagggcctc ccggcggccg tcgaggagcc gggagccggg gaggcgaagc cgtgaaggcg
    10981 ctcttcgcga tcctgaagcg cgagttcctc gcgtacttct tctcgccgct cgcctacgtc
    11041 gtcctgacgg cgttcctctt catcaacggc tacgtcttct acgcgatcct cctgtcgctg
    11101 aacagcccgg agacccctcg tcaggagctg atggcgctct tcttcaagaa cgtcttctac
    11161 tggatcttca tgatgctcgt ctcctcgatc gtggcgatgc gcctcgtctc cgaggagcgg
    11221 aagtcgggct cgatcgaggc gctgctgacc gcccccgtcg gcgagggcac cgtgatcgcg
    11281 ggaaagttcc tcggcggcct gacgttcttc ctcttcctct ggctcccgac ggtcctctac
    11341 ccggtcgcgc tgtcgcggta cggccggatc gacttcgggc cgatcgcctc gagctacctc
    11401 ggcatcgcgc tcttcggcgc gctcttcatc gcggccggga cgttcgcctc ggcgctgacg
    11461 aagaaccaga tcatcgccgc gatcgtcagc ttcgtgatga tcctcgcgct cttcctcgcc
    11521 ggggtcttcc gcgagctcgt caccgacccg aagctccggg aggggctcgg ctacctgaac
    11581 ctgatcgagc acatggacga tttcgcgcgc ggcctcgtcg acacgcggcg cgtcgtctac
    11641 gtcgtctccg cggtcgtctt cttcctcttc ctcgccacga aggccctcca ggggaacaag
    11701 gggaagtgag atgagcgaga gccgcaccgc ccgccgcgtc aacctcggcc tcgcgatcct
    11761 cgtcggcctc gggatcctcg ccctcgtgaa ctggctcggg gcgcgccact accgccgctt
    11821 cgactggacg agcgcgggcc tgtacaccct ctccccgaag tctgcggagg tgatgaaggc
    11881 gctgacgaag ccggtctccg cgaccgtctt cctcaccgag ggctccccgc tctacgccga
    11941 gacccaggag ctcctcaagc gctaccaggc ggcctcgccg ctcctcacgg tcgagacgct
    12001 caacccggag cggaacccgg cccgggcgca ggccttcctg aaggagatgg gcgacatccg
    12061 cctctccgtc gtcttccggt cgggggagaa gaagaaggtc gttccggtcg acgcgctcgc
    12121 ggactgggac ttctcccgcg ccgagatggg gggagagccc gcgctcaagg ggttcaaggg
    12181 agagcaggaa ttcaccgccg cgatcctctc cgtgacgcag gagcggagcc ccaaggtcgt
    12241 cttcacgacg gggcacggag agcggccgct ctcggggcgc ctctccgagg gctacgagcg
    12301 cgccgcggac ctcctgaagc aggacaactg caccgtcgag gagtgggaca gcctcgccga
    12361 ccagaaggtc cccgacggga ccgacctcgt cgtcctcgcc ggcccgcgcg ccgcgttcac
    12421 cttccccgag acggacgctc tcctggcgtt cctccggaac gggggccgtg cgctcctcct
    12481 cctcgacccc gagttcgccc ccggggccgg gaaccggctc gtcgacctcg gcctcgggcc
    12541 ggtcctcgag gcctacggga tccgcctcgg cgacgacatc gtcgtcgacc cgcggaacgc
    12601 cctgccgctg atgggcccgg agacggtcgt ctcgcgcagc ttccggtctc accccgtgac
    12661 gaagctcctc cagggcctcc ccgtcgtctt cccggtcatc cgttcggtcg gggccgtgga
    12721 gccggcgccg aaggacgtct cggtcacggt ccttgccgag acctccgccg aggggtgggg
    12781 agagacgaac ctggccgacc tggagacgcg ggtcgcgaag gacgagaagg acgtcgccgg
    12841 gccggtgccg ctggccgtcg cggcggagtc ggcgacggag aagaaggcgc ggctcgtcgt
    12901 cgtcggagac gccgacttcg cctcctcggg cgggatcgcg aacgccgcga acctctatct
    12961 cctctcctcc gccgtcaact ggctcctcga gcgcgagtcg ctgatctcga tcccgccgaa
    13021 gtcgaccgac caggtctcgg tctccctctc gcgcggagac gtgggccgca tcaccctcct
    13081 cgtcctcctc gtcctcccga tcgccgcgat cgccctcggg ctcgcggtct ggctccggag
    13141 gagacgctga cggagccgcc atgtcgacgc ggaagctcct gatcctcacc ggcgtcttct
    13201 tcgccctcct cgcgttcgtc gtcctctggg agcggcatca gccgacctcg gacgaggccg
    13261 cgaaggcccg aaagcggctc gtcgacctcg atccgaaaga ggtctcgcgg ctcaccctcg
    13321 aacgggccga acaggcgaag gtggagctcg tccgcgagga cgggcgctgg actctcgccg
    13381 gtccgaaggg aggcgccgcg gacgccgtca ccgccgacgg tctcgccgcc gatctcgccc
    13441 ggctcgacct cctgggcgag acgaggacgg agtttgaccc gaaggcgcac ggcctcgcct
    13501 cgccgcgggt ccgggtcacg gtcacgacga cggacgggaa ggcgcggacg atcctcttcg
    13561 gcgacccggt cccgggggcc gacgccacgg ccgcctccgc cgacggccgg ctcggcgcgg
    13621 tgcggttcgc gccgatcgcg cagctgacga agccgtacga cgagtaccgc tcccggagcc
    13681 tcctcgacgt cccgaccgcc gagatcgacc gcgtcacggt cgtccgcggg ccgaaccgcg
    13741 tcgtcgccgt ccgcgacggc gccgggtggc tcctcgagca gcccgttgcg gacctcgcgt
    13801 cgggctcgtt cgtcgaccag ctcctcgccg acctcgccgg cgtccgtgcg accgagttcc
    13861 cgaccgtcgc cgcggccgac atgccgcgga tcgggctttc tccgcctgcg gccgaggtcc
    13921 tcctcggcaa ggggaaggag gtcctcgccc ggctcgcttt cggcgcggcg aaggccgacg
    13981 ccgcggggaa gctctacgcc gcccgagacg gcgtggtgat ggtcgtcgac gaccgggcgc
    14041 aggagacgct cggaaaggag ctctcggcgt tccgcgaggg acgcctcctc ccgctcgaca
    14101 gctggctcgt cacgcgcgtc gtcttcgaga gcgaggccct ccgggccggg gcggagaagc
    14161 tggaaggagc gtggcggtcc gcgggccgcg aggtcgacgg gcgggtcccc gaagaccttc
    14221 tcgagcgcct cggccgggcc gaggtgcgcc gcttcgtggc cgcgaaggac ctcgcgtcga
    14281 tcgggctcgc gcccgtgcgc cggcggtctc ccgtcccggc gggctccgtc gaggtgctcc
    14341 tcgagaaggc gaagaagccc gttcggatcg tcttcttctc gacgccgtcg ctcgcggcgg
    14401 agcagctcgt ggcggcccgc gtcgacggcc ggggagatac tctcgtcgtc gacgcgtccg
    14461 tctgggacga cgtgcgctcc ttcgcggtcc gcctgaaaga ggcggcggcc gccgggccga
    14521 ctccgaccgc cgctccccgt ccgtaaggcg gcagaggaat cgccggggac gacgccgccc
    14581 cgaaccaatc cggtcgccct gcgtttccaa tcgggtacgc gtgcggcggg acgggccacg
    14641 aggcccggtc cgcggggcgc cgaaggagga gacgatgccg gagcgcccga ccgatccccg
    14701 ctgcgccgcg ccgcccccga gccgcccgag gaccgcggtc ctcgccgccg tcctcgggac
    14761 cgcgctggcg gcgttcccct gcgcggcagg cgaggtgatg gtcgtcggca cgccgcacct
    14821 cgccacgctg cggccggagc cgggaccgga gcgcctccgc gaggtcgtcg agcggctcgc
    14881 ctcctgggcg ccgacagtcg tctgcatcga ggcgatcccg ggcgatcgcg ttcgccagct
    14941 cgccggcgat cccgcggcgt ggggtgagct gctctcgacc ttcgcggtcg acgcgatccg
    15001 gctcgcgccc gagcagcagg cgcgcctgtc tgtcgacggc gccaccgcgc tccggctggc
    15061 tcgcgagctg gaagcccggc ccggatcgct cacggatgcg gagcgggtcc gtctcgtcgg
    15121 gctccagctc gccggccacg agccctggtc ggcgctgctc ggctggacgg tcctgacgga
    15181 cgaggagcga gccgccgccg cgaagagcct ggggcgtgcg gccgtcgagc gtctcggcgc
    15241 gctcgcgtcg tcccggaacg agatcgtctc cctcgccgtc cctctcgcgg cgcggctcgg
    15301 gcaccggcgg ctctgcatgg ccgacgtctt cgtcgacgag gtggcggtcc agacgctgga
    15361 ggaggacctc gtgcccctgt tccgggaccc cggcgtggcg aaggggctcg aggcgatcga
    15421 ggcggcgaag gccgcgcgct ggaagccgga ggagaaggac gggctcgtcc ggctcgtcgc
    15481 gtggatgagc gcgtccgact acgcggaggc ggaccggcgg acgcaatggg acctgttcgc
    15541 ccgcgccggc gtgccgaacc gagcggggga gcgccgcctc gccctgtggc acgcccggaa
    15601 cgccgagatc gcggcgcacg tctaccgggc ggcctccggc ccggacggtg cccggaccct
    15661 tctcctcatc ggggccgccc accgaccgtt cctcgaggcc gcgctgcggg cgcagccctg
    15721 gatcgacgtc gtcccggcgg agagggcgct cggcgtcgtg gggcgctagg acttcctctc
    15781 gggcgggtcc gtctcgccgc gggccgggcg gctcgcgacg gagcggtccc gctcggcggc
    15841 ctcacgctcg aggcgggcga cccgcgcctt cctctcctgc tccttgcggt cctcgacctt
    15901 ctccaccttc ttcttcgagg agccgaggaa cgcccctgcc gcgaggacga gcttgaggtg
    15961 gagctggccg gtgaatttcg ccccttcccc cttcgccccg acgaagctgg agcctccgag
    16021 cttcgcttcg gagaagtcgg cgtgggcgac gttcgccccc ttgaagagcg ctccttccag
    16081 cacggccccc acgagcgtcg agaagcggaa gtcggctccg gagaagtccg cctcctgagc
    16141 ctccgccccg agcaggttcg tccccgagaa atcggccccg ggcgcgagga cgcccgccat
    16201 ccgcgcccgc ttcaggttcg cgcggcggag cgtcgcgcct tcgaggtgcg cccgctggaa
    16261 gatccctcct tcgaggttcg cttcggtcag gtcggctcgg gagaggtccg cgtcggtgta
    16321 gttccccccg cgcaggttcg cgccgacgcc gttcgcctcg gcgagcttcg cgccggtgaa
    16381 gttcgggaac ttcagcttcg gcgtccggag gtcgagcttc tcgaggttcg cctgggtcag
    16441 gtcggtctcc tcgaggagcg ccccgaggag gatcgagcgg cgaaggtcgg cgtccttgaa
    16501 gcaggccttc ctcaggttgg cgtcggagaa gtcggccgcc tgcgcgatca cgcgctcgaa
    16561 gtcggaggag gtcaggtcgg ccccggtgaa gtcgacaccc gtcaggttcg ccccgcggaa
    16621 cgtgatccgc tgccccttca cgccccggaa gctcgtcccg gcgagcttcg cgtcccggaa
    16681 gaaggcgagc gtgagcgtcg acttgtcgaa cctgccgcgc tggtaggtga tgccgtcgaa
    16741 gacggcctgg gagaggtcgc actcttcgaa gacgcagccg tcgatcacga cgccgcggaa
    16801 gttcgccgcg acgagccggc tgtacgagaa gacgctgccg gagaacgtcg tcccttcgag
    16861 cttcgccgtc gagaggtcgc aggccgtcaa gttcgcctcg cgcaccgagg cgttcttgag
    16921 gttgacgccg cggagggcgg ctccgacgag ggagaccttg tcgagcgtct ggtactcgag
    16981 gtcggtgttc gagaggtcgg tacgctggag gctcgtcccg cggagggaag tcccctggag
    17041 gtgcgctccg gagaggttcg cgccgtcgag cttcgcgccg tcgagcttcg agcgcgagag
    17101 gtcggcgccg gcgaggttct tccccgagag gtcgaggccg ccgagggaga cgtccgtgag
    17161 gtcgggggtg atgcctgagt gctcggtcct ccactggttc cagtcggggc ccttcagcgc
    17221 ctccagatgc tcggagctgg ccatggcgcg gaagtctaac gtctagaatc gcgggatgag
    17281 cgccgtcgtt cccgtccgcg gagccgagag gatccgccgc gccctcctcg gctcctaccc
    17341 cctcgtctac gtacagtcct gggaggaggg gagagtcgag cgggccgtcg cgatgctcgc
    17401 gcagaagttc tacgaacggc ccgttccgtt cgccgtctgg acgtgcgtgg aggggtggac
    17461 gggcctcccc gaagccccga ccgagacgcg cgaccccgtc gcggccctcg aggcggtcct
    17521 ccgggcgaac ggaccgggct tcttcctgat gaaggacctt cccgcgctct tcgacgggcg
    17581 cgccgacgtc gtccggaagc tgcgcgacgt gtaccggcag gcgaaaggga aggggaagtt
    17641 cgtggtgctc gtctcgccgc ggctcatcct gcccgaggac ctcaagaagg aggtcttcct
    17701 cgtcgactac gacctcccgg acgaggcgga ggtgcgtaac gcgatctccg tcctcggcag
    17761 gcgctacctc ggggaggcgg ggctctcgcc cgccgacgcg gaccgggtcg tcacggtcct
    17821 gcgcggcctg acgctcgacg agctcgagca cgtcttctcg aaggtcttcg cccggactcc
    17881 gcgcttcgac gaggcgacgt tcgacgagct cctcgccgag aaggagcagt cctcccggaa
    17941 ggagggggtc ctcgagtacg tccctccgcg cttcaccctc gacgacgtcg ggggcctgga
    18001 gaacctgaag gattggctgc ggaagcggca gcacctcttc acgcgcgagg cggtcgaggc
    18061 gggcctcccg gttccgaagg ggctcctgat gatggggatg tcgggctgcg ggaagtcgct
    18121 ctcggtcaag accatcccgg ccctctggaa cctcccgctc ttccgcctcg acatgaacct
    18181 cgtcttcggc acggcgaacc ccgaggcgac gttccaccgc gccctgaaga cggtggaagg
    18241 gctcgcgccg tccatcctct ggatcgacga gatcgagatg gcgatcacgg gcgggcgcga
    18301 ggcggggggc ggcgacccgg ccctcgggcg gatcttctcc accttcctca cctggatgca
    18361 ggagaaggag gccctcgtct tcgtcgcggc gaccgcgaac cgaatccacc tcctgccggc
    18421 cgagttcatc cggaaggggc gcttcgacca ggtcttcttc ctcgaccttc cgaacgagac
    18481 ggagcggaag gcgatcttca ccgtccacct gaagaaggcg aaggccgacc tctcgaagtt
    18541 cgacgtcgtc ttcctcgcga aggcgacgaa gggcttcaac ggcgccgaga tcgaggccgt
    18601 ggtccaggcc tccgcgatcg acgccttcaa cgagaagcgg cccctcgacg aggacgccgt
    18661 ctcgcgcgtc atcacgaaca ccgttccgct ctcgcggacg atggacgagc agatcaaggc
    18721 gatcaagagc tgggcgcaca accgggcctt gtcggcctcg cgcgagagcg cctcctgacc
    18781 gggccgcgcc gccgggcgcg acgccccggg gcggtcagtt ccggcggggg tcgtcgccct
    18841 cgcggcggta cggggcgagg aggtcgatcc tcgactggag gctccggacc tggtcgagga
    18901 cccaggcgta gcgactctcg agggtctgca tggcgagcag ggcgtagcgc tcctccggcc
    18961 cgaggccgag gcggctcgcc gcttcgttga cgagcccctc gtccgagagc gacggcggaa
    19021 ggtgccgcgc ctcgctccgg ccgacggagt cggcgagacg ctcgacgtcg cgcgcgagga
    19081 ggcgccggag ctcgcgccgg cccgggccgc cggcccgccc cggcgcgaga ggggccaccg
    19141 gcgcttcctc cacgtcggcc agacggtagg gacggccgtc ccgctctgcc acgatccggt
    19201 accggaagag cccgaggagg acgatgttcg accgcccgtc ggggagcggc tcgtgctcga
    19261 cgatctcccc ggcgcagccg taggggagga gggccggccg cccgtcgggg aggggcgggg
    19321 cgtcgggggc gagcgtatgg aggccgagga ggcgctcgcc gtcgagggcg tccgcgagca
    19381 tcgcgcggta gcgcggctcg aagacgtgga acggcagccg ggtctgcggg aggaggaccg
    19441 cgtcgagagg gaagaggggg aggagccggc tcatcgcgcg ctcccgcctt ccgtccgagc
    19501 cgggagcgcg agggcccatt cgacgaccgc gtcgatctcc tccggcgaga gagtcccggc
    19561 gaacggcggc atcgtgctcg tcggatctcc ctcggcgacg agccgccgga gggcctccct
    19621 ttcggggccg tgcttccact tgccgtctgt caggtcggcg tacggccgcc cttcggcgaa
    19681 gcgggtccgt cccgccccgt ccgatccgtg gcaggcggcg caccgccgct tccagacgcc
    19741 ggccgcgggg tcggggtccg ccgtacagcc ggaggacgct ccggcggcga tcagggcgac
    19801 ggcgatcatg atccgaagcg aagctctcat gctatcttcc gcggccatgg cgaacaagga
    19861 cgaccgctac accgacaacg cctccggcaa gttctacgtc gacacccagt gcatcgactg
    19921 cgacgtctgc cgggtcaccg cgccgaacaa cttcgcccgc caggaggaga agggctattc
    19981 gtacgtcacg aagcagcccg acacccccga ggaagaggcc cagtgccagg aggcgatgga
    20041 ctcctgcccg gtcgaggcga tcgggaacga cggcgagtag cgccaccggc cggtcagcgt
    20101 ggggcggcgg cgccgccgcc cccggccgcg ccgagcgccg cccgggcgtg atagctcgag
    20161 cggacgagcg gccccgcctc cacgacggcg aaccccatcg cccgcccctc gtcgcgcagc
    20221 cgcgcgaact cctccggcgt ccaccacctc tcgaccggca gtctccgcgc gtgcggctgg
    20281 aggtactgcc ccacggtgag cgtgtcggtg ccggcggcgc gcaggtcgcg catcgtctcg
    20341 agcacctcgt cgaccgtctc cccgaggccg accatgagcc cgctcttcac gcgcatcgcc
    20401 ggggccgtcc cgtcccgcca ggcggcggcg cgcgagagca gctcgagcga gcgggcgtag
    20461 atcgcgtcgg ggcggacccg ccggtagagg cgcgcgaccg tctcggtgtt gtggttgagg
    20521 acctccggag cggcctcgag gacggtcctg agggcctcct cgttcccgag gaagtcgggg
    20581 atcagcacct cgacggtcac ccccggcgtc gtc
//
LOCUS       DAOTEZ010000018        20035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_1775_length_20035_cov_7.061662, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000018 DAOTEZ010000000
VERSION     DAOTEZ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 20035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20035
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1775_length_20035_cov_7.061662"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="P5164_01735"
     CDS             complement(<1..196)
                     /locus_tag="P5164_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009140248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY42642.1"
                     /translation="MTGPRVTVIGGGFAGVECAHQLARRGHAVRLLEMRPARTTDAHE
                     TDRLAEMVCSNSFRSDNPGNA"
     gene            251..955
                     /locus_tag="P5164_01740"
     CDS             251..955
                     /locus_tag="P5164_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRY42643.1"
                     /translation="MTFERELYDEAYWRGLHPHHWFKNPPRKYAERDRDVLRVVAPRP
                     SDVVLELGSARGDVSFLLARHAREVIGVDAAPEAIAMAEEARARLGIESVRWLEADVA
                     DLAPVPDRSVDAVAAIDLVEHVDDPTLEAMLRECRRVLRPGGRLGIYTPDRAHYVERM
                     KAHDFVLKQFPQHIAVRRVAEYRRLLAGAGFSIDLETWSVSPFPLVRWVERALAPLPL
                     LGPTFRYRILLRAVAP"
     gene            966..1676
                     /locus_tag="P5164_01745"
     CDS             966..1676
                     /locus_tag="P5164_01745"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY42644.1"
                     /translation="MPEPPDFAPNAAVYDDAYYAAMYRPHWFLRNARKYRERDAALLR
                     ALRPHPAMRLLEVGSARGDTAFFFAPLTARVVGIDAAPVAVEAARAAAVARGLENVTF
                     AVADARDLSAFAHASFDAVLLADFVEHVTDDVLVPCLAEARRLLVPGGALALYTPNRD
                     HWAERVKAGIPGLQQEDHIAVRRAAEVVRLVAAAGFSVEDLFFSASPYPFLGAVDRLF
                     AGAAVCRFRTVLRALRPV"
     gene            1775..>2567
                     /locus_tag="P5164_01750"
     CDS             1775..>2567
                     /locus_tag="P5164_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/gamma crystallin-related protein"
                     /protein_id="HRY42645.1"
                     /translation="MSRRSLLLFLLLPALAPAADAAAQGTGVALYEGTRLSGSRITLD
                     RDVFDLDETSFAGRRARSVAVSPGCRATLFELSGYRGASVEVTGRLDDLSVTRLGSRG
                     VSSARIACTGSLGSTGSAPWGAGTRGVTLFRDSGFKGDSETFSSDVPDLSRTRIGARQ
                     ASSVDVPEGCVAILFTGTGYSGRSTELRQAHDNLRLADVGNDAVSSLRVDCRGTGGST
                     IDSGWSAGGRGVTLFRDSGFKGDSETFSTDVPDLSRTRVGARQASS"
     assembly_gap    2568..2667
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2668..3437
                     /locus_tag="P5164_01755"
     CDS             <2668..3437
                     /locus_tag="P5164_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta/gamma crystallin-related protein"
                     /protein_id="HRY42646.1"
                     /translation="EVPEGCTAELFTGTGFSGRSTVFREAHEHLRLTEVGNDAAASLR
                     VDCRGAYRPGGGRGPGASRRGVTLFRDSGFRGESETFSSDVPDLSRTRVGARQASSIE
                     VPEGCTAELFTGTGFSGRSTVFQEAHDNLRLTDVGNDAAASLRVDCGRRRFRPADGRG
                     AGYGPAGVTLFRDKDFEGEAESFRSDVPDLSRTRVGARQASSIAVPSGCRVTLFGEPG
                     YRGRSATFDADHDDLRGTPIGNDAAQSMRVDCGSARW"
     gene            complement(3474..6092)
                     /gene="topA"
                     /locus_tag="P5164_01760"
     CDS             complement(3474..6092)
                     /gene="topA"
                     /locus_tag="P5164_01760"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRY42647.1"
                     /translation="MSKSLVIVESPAKAGTLGKFLGKDFTVHACYGHVRDLPRKGISI
                     DREAGYEPTYEVLPGKQKTIADLKRLAKGAEDVYLAADPDREGEAICWHLHELLRPSA
                     PNARFHRAEFNEITKSAIQKAIASPGEIDADRVAAQQARRIIDRLVGYEVSDLLWKKV
                     WRGLSAGRVQTVALRIIVEREAERERFVPVPYFSVPLQLAKGEALFPARTVAWRGAKL
                     KFDGTDPRLATREAAEEVLGHLRGATLRVASVEAREKRTNPVPPFTTSKLQQGAARTL
                     GFTVRRTMQVAQRLYEGKTIAGRGSVGLITYMRTDSTRVSEEALGAVRSYIGATFGAD
                     ALPAEPRRFKQKKDAQDAHEAIRPTMMDLPPEAVAGDLSADELKLYRLIWSRFVASQM
                     APSVSDVTTVEIEATRPGSSEVAGVRASGSVLKDPGWLRVYGQVAEGEEAEGEGNGDA
                     EEGKVRLPQLGEGDLLRLAEAAVEDHETQPPPRFNEASLVKFLEENGIGRPSTYAEIL
                     RKIEDREYVRKKDRRFLPTLLGRLVIDLMREGFDDFFQTESTARMEEELDEVEEGKLD
                     WRKAVGEFDARFTKDRDRALKKMISVKAGLALSEVKKRLPGFPLPEGVGEKCPKSGDE
                     LRLRMGKNGLFVACAGYPGCDYTVDVPEPEDDPVDASELEGQTCDECGSPMKLRTGRD
                     GSAFLGCTAYPNCRFTVAVKVANGKAEARPDVPTGEKCPLCGHDLVARHGRFGDYVGC
                     SNYPACRYRPPKPVTSTNVACPDCGTGEVLVRKGRFGPFYGCSNYPTCTRNFRARPVP
                     KACPACGAPYLLVRDRKAGAFYVCEKEGCGFDAPAADLEAWAFVTKVPEAAAVAAAAA
                     AAAEKKKPAAKRARKG"
     gene            complement(6118..7242)
                     /gene="dprA"
                     /locus_tag="P5164_01765"
     CDS             complement(6118..7242)
                     /gene="dprA"
                     /locus_tag="P5164_01765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRY42648.1"
                     /translation="MPTMRMPSDIVLSWGLRVRGTRARALARLLLAAGSVEKVLSLSP
                     AEAVALGGEPGCEELLGSPGGAELGAARDRLEALGARTIAICDAEYPALLKEIADPPP
                     VLFVRGGHLSSAPVVGVVGSRRASRTGLEAARRLSAGLARAGVVVVSGFAHGIDAAAH
                     AAALDEEGETWGVLGCGLDVPYPAAHGRLRERIVAGGAFLTELPPGTPPEPWHFPARN
                     RIIAGCARLVVVVEAAPRSGSLITARCATEAGRDVGAVPGPVLGDLSAGTNALLKDGA
                     ILVRDAADVLRELPDEDLVRLRTAGAPAARDASPAPADPDAARVLLALDPVEPRAADE
                     LAAATGLSAARLSAALVLLELEGLAEALPGALFARKAPRA"
     gene            7279..7668
                     /locus_tag="P5164_01770"
     CDS             7279..7668
                     /locus_tag="P5164_01770"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY42649.1"
                     /translation="MRHSRRAPLAKGLLLAIVVSLTGCASASRDAANAADQKSYGARM
                     AEKGFWREALFRFERAARLAPDDPEVLNNLAVACEATGETARALTAYKRALELRPEDS
                     RIKRNYARFAEYYTSLQRTSGPMAAPK"
     gene            7665..8369
                     /locus_tag="P5164_01775"
     CDS             7665..8369
                     /locus_tag="P5164_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42650.1"
                     /translation="MRRFSTLAALLLLLGAPAGPLLAGSREVKLRLPLRPKLAVTGRE
                     RLSLAPFLVASKLSERKESQRVKDLDLDLEFRRYLGKQIARRTKMNVVTMPADVKLPS
                     TELKALGEASAYWKELGARVGADVLLSGIVDFRVEDKSGYKTQEYQSPIDGRTYYRQD
                     FVESTGFTFEVTVLAFDGRTGAKLFQETFKDQKEKSRRAADEMVGLFENLFSLENQLL
                     GLFVVREREANRYVFD"
     gene            8356..11232
                     /locus_tag="P5164_01780"
     CDS             8356..11232
                     /locus_tag="P5164_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRY42651.1"
                     /translation="MCSTRRRAARLVPAGALAALLLVAAGPAPAQGQNKVVYDRFDWK
                     IYSSTHFRIYYYGKGEEALGKVASMAESAYDDLSRRLNYQVPKPIPLLYYVSHAEFEQ
                     NNVILNFIPEGVGAFAEPARNRMVLPIDLPDDKLQNLIAHELTHIFQYEILFGGRLSR
                     AITANIPTWFMEGMASYFAKDEDEKDRMFIRDAVNSDQIPPITRINITGYPAYRFGHA
                     FFDFVEAEWGKDAVRELVFEFRSFLGRDVAVALRRTFDVEPDDFDLKFRRYLRRKYLP
                     LLAVKGEASEYGERFRVGPWERPSYEVGVAPSPSGDFVATLTTYKEDVDIALLSTKSR
                     KLWKNLSPGRTTKYEYISAQFLTQAPESGRDLSFAPDGDRIAVFARRERGRQLYIFSA
                     RGGGILERIPVSPDMPVGPSFSPDGRAVVFSGISGTSRDIFRLDLETKAVTNLTSDAA
                     YDTAPVYSPDGKYLYYTKSIGEFQKIVRFPVDDPKKVEQVTWGEGNDDDAALSPDGER
                     LYFASSRGGIFNVYGQDLKTGEIVQYTDVIGAAFGPASYIGPDGQEKVVFSGYQAGRI
                     QLYVADAKKPFRRLEEKALPPAPVDAAGVPGFVPSIEVAIDPEKVEKSRFALFVDDVG
                     AYAGVQSDGTFVSSARISLSDYLGDRRAFVIWDTLAGYSNVNAIYFDLKRRFQWGLQA
                     YDSRIYFLGYNQIGELTSDRRFLRETGVNVLGVYPFSRYTRAEGSLGFVSRSIDAPYA
                     YTNADGTQGQIVLPRKDNTMVAGLGFGYDATQFKAFGPHSGQALSVNYRYLPDFQDGG
                     TLSQEVSAEARLYLPVTRRSNFAIRAFGGAASGASPAVYAFGGLDNLRGYDYRSFIGN
                     RIAYLNAEFRFPLIDALITGIGLNVGGVRGRFFFDVGGAWLEGEDFKWWDDGRLRDGR
                     AAWGAGFTAYFLGIPWNVDFARPFDGRSNLAGWQTTFYIGPGSF"
     gene            11300..12061
                     /locus_tag="P5164_01785"
     CDS             11300..12061
                     /locus_tag="P5164_01785"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HRY42652.1"
                     /translation="MKVRVLGAYGGSSPWHRQTSFLLDGTVALDAGALTQSLTLDEQA
                     RVRSILVTHSHLDHVASLPFLVENVFGRTEGPIEIIGPQDVTSLLQGHLFNDAIWPDF
                     TRIPTHFLPAVTFRTVEEGTPFRVNGLQGLALSVSHVVPTFGYLLWDDRSCVVFSGDT
                     GPTEALWAEARRRDDLKAIFVECSFPDSMAEIAEVSRHLTPATLALEMRKFPAGVPVN
                     LYHMKPPTLPQLRADVAALSEPRVRILDDGDELEF"
     gene            complement(12266..13378)
                     /locus_tag="P5164_01790"
     CDS             complement(12266..13378)
                     /locus_tag="P5164_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42653.1"
                     /translation="MSAVREALETLGHPGGTLPEPRRLAELFVRFQAQVPLRRAGCGT
                     AEERLAGWLEAGAGLCGEARTDAFTTLAAEAGFALEAGHARGTAGDPRTVLVADGGRL
                     LFDVSFPLPLPVSLDPPAAELATGYGTLRVRPAAAGEEVLLETRGEERLLYRVDRDPA
                     SVSDSAAKGSSEETELFRLLDDRLLRFRGGVLEVSDAWSRLRVPFPARDADGLEALFG
                     PPAPPDDGREAAGQPPTLAVYLASGARPEALRALLADPESFAGTLPEGWAATEVEARA
                     DGFAWRVREGDDLLRTERIRVVPDGLVLEAEGPLALFRSRTLRLEPRPEGTRLRLLAT
                     LRDPVPPRGLPEGTRRRLVFELANELLALDRVAAGG"
     gene            complement(13398..13985)
                     /gene="pdxT"
                     /locus_tag="P5164_01795"
     CDS             complement(13398..13985)
                     /gene="pdxT"
                     /locus_tag="P5164_01795"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HRY42654.1"
                     /translation="MPVGVLALQGDFEAHGAALARLGIDSRPVRTAEEVRASAGLVLP
                     GGESTTMWKLMEGTGIAEAIVAVARRGDPLFVTCAGAILSARRITSPDRDGLGLLDAT
                     AVRNAYGRQLDSAVVTLTDLDRSVLGGEPLEAVFIRAPKLADLGPGVEVLARRNGDPV
                     LVRDGNLVAATFHPELAADARVYDLVFGAAAARAA"
     gene            complement(13988..14899)
                     /gene="pdxS"
                     /locus_tag="P5164_01800"
     CDS             complement(13988..14899)
                     /gene="pdxS"
                     /locus_tag="P5164_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HRY42655.1"
                     /translation="MSSTPIYSSPFTSDAFRVKVGLAEMLKGGVIMDVVNAEQAKIAE
                     DAGASAVMALERVPADIRRDGGVARMSPVSKIREIEAAVSIPVMAKCRIGHIAEARVL
                     ESLEVDFIDESEVLTPADEENHVYKHDFKVPFVCGCRNLGEALRRIGEGAAMIRTKGE
                     AGTGDIVHAVRHLREVTRQMKSLTLLSRDEWMAAAKQLAAPFELVVWVAEHGKLPVPN
                     FAAGGVATPADAALCRILGAEAIFVGSGIFKSDDPANRARAIVEATTHWDDPARVAAA
                     SEKLVEGMKGIEAGKLVEAELLQTRGW"
     gene            complement(14914..16419)
                     /locus_tag="P5164_01805"
     CDS             complement(14914..16419)
                     /locus_tag="P5164_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRY42656.1"
                     /translation="MTDRLPAADGAPGRIPLYRTIADGIIRDIRRGRFQEGSRLPASR
                     ALARTHEVNRATVHQALQVLKREGWIETNKGGGTRVARRSLAGPMVGPAPSWDRISSA
                     LLGRMLAEAGVPDGPLDGAHDLARLAPEEKEFPAEAFAKVLAEVARDGALLAYGSPFG
                     YRPLRETLAARLERRGVPADADSLLIVNGAQQGLDLIGRALVDPGDPVAVESPSYSGA
                     LSLLRAQGARLLGVPLDAEGPAMEPLRGALAERPKFLYTIPDFQNPTGLLATAARRRE
                     LVAAATAAGVLVVEDAFDADLLVEGELPPPLAALAPGHVVHIGTISKALFPGVRVGWI
                     SGPREFVHRLAALKRIGELTSPPVLQAALNVFLSRGDYDRHLLRVKARLKARLALAHK
                     TVLRSFPVGTRCERPLGGWVLWVDLPEDVSGRELAEAARGAGVLVSPGGDYAPDRADV
                     PSLRISVARSSGAELEKALESLGVLARAAVRRAHRRAPRAEERREEALIQI"
     gene            complement(16504..16788)
                     /locus_tag="P5164_01810"
     CDS             complement(16504..16788)
                     /locus_tag="P5164_01810"
                     /inference="COORDINATES: protein motif:HMM:NF016271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HRY42657.1"
                     /translation="MAISRERANDLSHRVLEALRKTPGVELTAEAEFVRNRIASALQE
                     WEKESDGLSAEARRRVLARGRRVPEGSREFDQLLSEELRRLWDERAARGE"
     gene            complement(16808..17461)
                     /locus_tag="P5164_01815"
     CDS             complement(16808..17461)
                     /locus_tag="P5164_01815"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HRY42658.1"
                     /translation="MRFFLDTVDADEARRVKEWGLLDGAVVRADAVLASGRDYRKAVS
                     EISQVSDGPVFAAVAAGEPKGMYKEARELAKLGKAIVVRLPMTREGLRVVRLLNDEQI
                     AADVHLLATPLQALLAARAGAAYASVSVSGLDGAGQVGMDVVDAVVRVYDNYGLQTQI
                     VVDAVQNPVHALDAAAMGADAAAIPFPVLDRLFDHPLTAQGVAAIRAHAGEGPRRVH"
     gene            complement(17471..>20035)
                     /gene="gyrA"
                     /locus_tag="P5164_01820"
     CDS             complement(17471..>20035)
                     /gene="gyrA"
                     /locus_tag="P5164_01820"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRY42659.1"
                     /translation="VSIEEEIRRSYLDYAMSVIVGRALPDVRDGLKPVHRRVLYGMWE
                     QGNRHNRPYKKSARIVGDVMGKYHPHGDASIYDTAVRMAQDFSMSEPLVDGQGNFGSV
                     DGDSAAAMRYTEVRLTALAEELVGEDIDKETVDWSPNYDGSLQEPNVLPAKFPNLLVN
                     GAEGIAVGMATKIPPHNLREACDAAILLLEKPDAGLDELLRLLPGPDFPTGGILLGRR
                     GVVEAYRSGRGIIQVRGRAEIEASTRADRESIVITEIPFQVNKARLIEQIADLVNEKR
                     LEGISAIRDESDRDGMRVVVDLKKGENANVLLNRLYQLTPLQSSFGIIFLAIVEGQPR
                     VLPLKEMLRHFLDHRRQVVVRRTRFDLAKAEARAHILLGLALALSNLDRVIAIIRGSK
                     DPEEAKRRLTEEVAVDLPALMAFLDLPALDLSSRARRDGELVRLDEVQAQAILEMRLQ
                     RLTGLEREKIVAEYKDVLALIADLKDILARPERVAAIIRAELTDIRTRFGCDRRTEIS
                     TLERDLADEDLIKDEAVVLTVTRGGYAKSTPLSVYREQKRGGKGRTGAAATEEDVVEH
                     LFVASTHDSLLVFTDRGRVHCVRVWDVPSAGPAARGKALVNLIQVEPGDTVAAMTTTR
                     GFPEDRFLLFATKRGLVKKTVLSAYGNVRAAGIIAINLEEEDELLSVQVTDGARQVFL
                     GTRNGMGIKFTETDVRPMGRDTTGVKGIELREGDEVVEMDLVDEEATLLAVTELGYGK
                     RTDVAEYRHQSRGGTGVINVRVTEKNGPVVGIKSVGEDDQLLVITRMGMMIRFAAAGV
                     RETGRAAQGVKVIDLDEGDTVVAVAKLASLGERDEAAEDEDAGAPGTPSGDEPESE"
BASE COUNT         2647 a         7403 c         7017 g         2868 t
ORIGIN      
        1 cggcgttccc cgggttgtcc gaccggaagg agttcgagca gaccatctcg gcgaggcggt
       61 cggtctcgtg ggcgtccgtc gtccgcgcgg ggcgcatctc gaggagcctc acggcgtggc
      121 cgcggcgcgc gagctggtgc gcgcactcga cgccggcgaa gccgcctccg atcaccgtca
      181 cgcggggtcc cgtcatgcgg ccctcattgt agggaaccgg cggcccggcc ccgccgctac
      241 actcccgcgc gtgacgttcg agagggagct ctacgacgag gcctactggc gcggcctcca
      301 cccgcaccac tggttcaaga acccgccccg caagtacgcc gagcgggacc gcgacgtcct
      361 ccgggtcgtc gcgccgcgtc cctcggacgt cgtcctcgag ctcggctcgg cgcgcggcga
      421 cgtttcgttt ctcctcgccc gccacgcccg ggaggtcatc ggcgtcgacg ccgcgcccga
      481 ggcgatcgcg atggccgagg aggcccgcgc gcgcctcggg atcgagagcg tgaggtggct
      541 cgaggccgac gtcgccgacc tcgcgccggt ccccgaccgg agcgtcgacg ccgtcgccgc
      601 gatcgacctc gtcgagcacg tcgacgaccc gacgctcgag gcgatgctcc gcgagtgccg
      661 ccgcgtcctc aggcccggcg gccgtctcgg gatctacacc cccgaccgcg cgcactacgt
      721 ggagcggatg aaggcgcacg acttcgtcct gaagcagttc ccgcagcaca tcgcggtccg
      781 gcgcgtcgcc gagtaccggc gcctcctcgc cggcgccggc ttctcgatcg acctcgagac
      841 ctggagcgtc tcccccttcc cgctcgtccg gtgggtcgaa cgcgctctcg cgccgctccc
      901 gctcctcgga ccgacgttcc gctaccggat cctgctccgc gcggtcgcgc cgtaggatcg
      961 gctcgatgcc cgagccgccc gacttcgccc cgaacgccgc cgtctacgac gacgcgtact
     1021 acgccgcgat gtaccgcccg cactggttcc tccgcaacgc gcggaagtac cgcgagcggg
     1081 acgcggcgct cctgcgcgcc ctccggccgc acccggcgat gcgcctcctc gaggtcggct
     1141 ccgcgcgcgg cgacaccgcg ttcttcttcg cgccgctcac cgcccgggtc gtcgggatcg
     1201 acgccgcccc ggtcgcggtc gaggccgcgc gcgcggcggc cgtcgcccgg gggctcgaga
     1261 acgtgacgtt cgcggtcgcc gacgcgcgag acctctcggc cttcgcccac gcctcgttcg
     1321 acgccgtcct cctcgccgac ttcgtcgagc acgtgacgga cgacgtcctc gtgccgtgcc
     1381 tcgccgaggc gcgtcgcctg ctcgttcccg gcggcgccct cgcgctctac acgccgaacc
     1441 gcgaccactg ggcggagcgg gtcaaggcgg gaatccccgg cctccagcag gaggaccaca
     1501 tcgccgtccg ccgagcggcc gaggtcgtcc gcctcgtcgc ggcggcgggc ttctccgtcg
     1561 aggacctctt cttctccgcg agcccgtacc cgttcctcgg ggccgtcgac cggctcttcg
     1621 ccggtgcggc ggtctgccgc ttccggaccg tcctccgcgc gctccgtccg gtctgaagcg
     1681 ccctcgggcg cggacgccgg ccgcggcacg cgccttgtaa ggaccgagcg ccgagggcta
     1741 ccctcggccc atcgacgaga gaaaggacgc cccgatgtca cgacggagcc ttctcctgtt
     1801 cctccttctc cccgccctcg ctcccgccgc ggacgccgcc gcgcagggca ccggcgtcgc
     1861 cctctacgag gggacccgcc tgtccggcag ccggatcacc ctcgatcgcg acgtcttcga
     1921 cctcgacgag acgtcgttcg cgggccgccg cgcgcgctcc gtcgccgtct ctcccggatg
     1981 ccgcgccacg ctcttcgagc tctccgggta ccggggcgcg agcgtcgagg tgacgggccg
     2041 cctcgacgac ctctccgtca cccggctcgg ctcccggggg gtctcctcgg cgcgcatcgc
     2101 ctgcaccggg tcgctcggca gcaccggctc tgcgccctgg ggcgccggga cgcgcggcgt
     2161 cacgctcttc cgcgactccg gcttcaaggg cgactcggag acgttctcct ccgacgtccc
     2221 cgacctctcc cggacccgca tcggcgcccg gcaggcgagc tccgtcgacg tccccgaagg
     2281 gtgcgtggcg atcctcttca ccgggacggg ctattcgggc cgctccacgg agctccggca
     2341 ggcgcacgac aacctccgcc tcgccgacgt cggtaacgac gccgtctcct ctctccgcgt
     2401 cgactgccgc ggcacgggcg gctccacgat cgacagcgga tggagcgccg gtgggcgcgg
     2461 cgtgacgctc ttccgcgatt ccggcttcaa gggcgactcg gagaccttct cgaccgacgt
     2521 gcccgatctc tcccgcaccc gcgtcggcgc ccgccaggcc agctccannn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnntcg aggtccccga ggggtgcacg gccgagctct
     2701 tcaccgggac cggcttcagc ggccgctcca ccgtcttccg ggaggcgcac gagcacctcc
     2761 ggctcaccga ggtcggcaac gacgccgcgg cctcgctccg ggtcgactgc cgcggcgcgt
     2821 accggccggg cggcggccgc ggccccgggg cctcccggcg cggggtgacg ctcttccgcg
     2881 actccggctt ccgcggcgag tcggagacgt tctcctccga tgtccccgac ctctcccgca
     2941 cccgcgtcgg cgcccgccag gccagctcca tcgaggtccc cgaggggtgc acggccgagc
     3001 tcttcaccgg gaccggcttc agcgggcgct ccaccgtctt ccaggaggcg cacgacaacc
     3061 tccgcctcac cgacgtcgga aacgacgccg cggcctcgct ccgcgtcgac tgcgggcgcc
     3121 gacgcttccg ccctgccgac ggacgcgggg ccgggtacgg ccccgcgggg gtgacgctct
     3181 tccgggacaa ggacttcgaa ggggaggccg agtcgttccg ctccgacgtg cccgacctct
     3241 cgcggacgcg cgtcggcgcc cgacaggcga gttccatcgc ggtcccttcg ggctgccggg
     3301 tgacgctctt cggcgagccg ggctaccggg gccgctccgc gacgttcgac gcggaccacg
     3361 acgacctccg cggcacgccg atcgggaacg acgccgcgca gtcgatgcgc gtcgactgcg
     3421 gctccgcgcg ctggtgagga gaggaacgcg gggcgccgca cggcgccccg cgttcagccc
     3481 ttccgggccc gtttcgccgc gggcttcttc ttctccgcgg cggcggccgc cgccgcggcc
     3541 acggccgccg cctcgggcac cttcgtgacg aaggcccagg cctcgaggtc cgccgccggg
     3601 gcgtcgaagc cgcacccctc cttctcgcag acgtagaagg cgcccgcctt ccggtccctc
     3661 acgaggagat agggcgcgcc gcacgcgggg caggccttcg gcaccggccg tgcgcggaag
     3721 ttcctcgtgc acgtcgggta gttcgagcag ccgtagaacg gcccgaagcg acccttccgg
     3781 acgaggacct cgcccgtgcc gcagtcgggg cacgcgacgt tcgtcgaggt gacgggcttc
     3841 ggcggccggt agcggcaggc ggggtagttc gagcagccga cgtagtcgcc gaaccggccg
     3901 tgccgggcga cgaggtcgtg gccgcagagg ggacatttct ccccggtcgg cacgtcggga
     3961 cgcgcctcgg ccttgccgtt cgccaccttc acggcgaccg tgaagcggca gttcgggtag
     4021 gccgtgcagc cgaggaacgc ggagccgtcc cgccccgtcc ggagcttcat cgggctgccg
     4081 cactcgtcgc aggtctgccc ctccagctcc gaggcgtcga ccgggtcgtc ctccggctcc
     4141 gggacgtcga ccgtgtagtc gcacccggga tagccggcgc aggcgacgaa gagaccgttc
     4201 ttccccatcc ggaggcgcag ctcgtcgccg ctcttcgggc acttctcgcc gaccccctcg
     4261 gggagaggga agcccgggag gcgcttcttc acctcggaga gggcgagccc cgccttcacc
     4321 gagatcatct tcttcagggc gcggtcgcgg tccttcgtga agcgcgcgtc gaactcgccg
     4381 accgccttcc gccagtcgag cttcccctcc tcgacctcgt cgagctcctc ttccatccgg
     4441 gccgtcgact cggtctggaa gaagtcgtcg aacccctcgc gcatcaggtc gatgacgagg
     4501 cgtccgagga gcgtcgggag gaagcggcgg tccttcttcc tcacgtactc gcggtcctcg
     4561 atcttccgga ggatctcggc gtaggtggag gggcggccga tgccgttctc ctccaggaac
     4621 ttcacgagcg aggcttcgtt gaaccggggc ggcggctgcg tctcgtggtc ctcgaccgcg
     4681 gcttcggcga ggcgcaggag gtccccctcg ccgagctgcg gcaggcggac cttcccttcc
     4741 tcggcgtcgc cgttcccctc cccctcggcc tcctcccctt cggccacctg gccgtagacg
     4801 cggagccagc ccgggtcctt gaggaccgag ccgctcgcgc ggacgccggc gacctcggag
     4861 ctcccggggc gggtcgcctc gatctcgacg gtcgtgacgt ccgagacgga gggcgccatc
     4921 tgcgaggcga cgaagcggct ccagatcagc cggtagagct tcagctcgtc ggcggaaagg
     4981 tcgcccgcga ccgcctccgg ggggagatcc atcatcgtcg gccggatcgc ctcgtgggcg
     5041 tcctgggcgt ccttcttctg cttgaaccgc cgcggctcgg cggggagagc gtcggcgccg
     5101 aacgtggccc cgatgtacga ccggaccgcg ccgagcgcct cctcggagac gcgcgtcgag
     5161 tcggtacgca tgtaggtgat gaggccgacc gagccgcgcc ccgcgatcgt cttcccctcg
     5221 tagaggcgct gcgcgacctg catcgtccgc ctcacggtga agccgagggt ccgggccgcc
     5281 ccctgctgca gcttcgacgt ggtgaagggc gggaccgggt tcgtccgctt ctcgcgcgcc
     5341 tcgaccgagg cgacgcggag cgtggcgccg cgcaggtgcc cgaggacctc ctcggccgcc
     5401 tcgcgcgtgg cgaggcgcgg gtcggtcccg tcgaacttca gcttcgcgcc gcgccaggcg
     5461 accgtccggg ccgggaagag ggcctccccc ttcgcgagct ggagcgggac ggagaagtag
     5521 gggacgggga cgaagcgctc ccgctcggcc tcgcgctcca cgatgatccg gagcgcgacc
     5581 gtctggacgc ggcccgcgga gagcccccgc cagaccttct tccagaggag gtcggagacc
     5641 tcgtagccga cgagccggtc gatgatccgg cgggcctgct gagcggcgac gcggtccgcg
     5701 tcgatctcgc ccgggctcgc gatcgccttc tggatggcgc tcttcgtgat ctcgttgaac
     5761 tcggcgcggt ggaagcgggc gttcggggcc gaggggcgga ggagctcgtg gaggtgccag
     5821 cagatcgcct cgccctcgcg gtcggggtcg gcggcgaggt agacgtcctc cgcccccttc
     5881 gcgaggcgct tcaggtcggc gatcgtcttc tgcttgccgg gcaggacctc gtaggtcggc
     5941 tcgtagccgg cctcgcggtc gatggagatc cccttgcggg gcaggtcccg gacgtggccg
     6001 tagcaggcgt ggacggtgaa gtccttcccg aggaacttcc cgagcgtgcc ggccttggcg
     6061 ggagactcga cgatgacgag agatttcgac atgcggccgg ctacgatgcc acggttgtca
     6121 agcccgcggc gccttcctgg cgaagagcgc ccccggaagg gcctcggcga gcccctccag
     6181 ctcgaggagg acgagagccg ccgagaggcg ggccgccgag aggccggtcg cggccgccag
     6241 ctcgtccgcg gcccgcggct cgacggggtc gagcgcgaga aggacccgcg ccgcgtcggg
     6301 gtccgcaggc gccggcgagg cgtcccgggc cgcaggcgcc cccgccgtgc ggagccggac
     6361 gaggtcctcg tcggggagct cacggaggac gtccgccgca tcccggacca ggatcgcgcc
     6421 gtccttcagg agggcgttcg tcccggccga caggtccccc aggaccggcc ccgggacggc
     6481 ccccacgtcc cgtcccgcct cggtggcgca tcgcgccgtg atgagcgacc cgctccgcgg
     6541 tgccgcctcc acgacgacga cgagacgggc gcagccggcg atgatccggt tgcgggccgg
     6601 aaagtgccac ggctcgggag gggtcccggg cggcagctcg gtcaggaagg cgccccccgc
     6661 cacgatccgc tcgcggagcc ggccgtgcgc ggccgggtag gggacgtcga gtccgcagcc
     6721 gaggacgccc caggtctcgc cctcctcgtc gagggcggcc gcgtgggcgg ccgcgtcgat
     6781 cccgtgggcg aagcccgaga cgacgacgac ccccgcgcgc gcaaggccgg ccgaaaggcg
     6841 ccgggccgcc tcgaggccgg tgcgcgaggc gcggcgcgac ccgacgaccc cgacgacggg
     6901 ggcgctcgag aggtgcccgc cgcggacgaa gaggaccggc ggcgggtcgg cgatctcctt
     6961 caggagcgcc gggtactcgg cgtcgcagat cgcgatcgtc cgtgctccga gcgcttcgag
     7021 gcggtcgcgg gcggcgccga gctccgcgcc gcccggcgag ccgaggagct cctcgcaccc
     7081 gggctccccg ccgagcgcga cggcctccgc cggggagagg gagagcacct tctcgaccga
     7141 gccggccgcg agaaggaggc gggccagcgc ccgggcgcgc gtcccccgga cccggaggcc
     7201 ccacgagagg acgatgtcgg acggcatccg catggtgggc atcttgcgta aagtccgctg
     7261 actataatgc cgtcctccgt gagacacagt cgccgagccc ccctcgccaa gggcctcctc
     7321 ctcgcgatcg tcgtgtccct gacgggctgc gcttccgcct cgcgggacgc cgccaacgcc
     7381 gccgaccaga aatcctacgg ggcccggatg gccgagaagg gcttctggag ggaggcgctc
     7441 ttccgcttcg agcgggctgc caggctcgct ccggacgacc ccgaggtcct gaacaacctc
     7501 gccgtcgcct gcgaggcgac gggggagacg gcccgcgccc tgaccgccta caagcgtgcc
     7561 ctcgagctcc gccccgagga ctcccggatc aagcgcaact acgcccgctt cgcggagtac
     7621 tacacctccc tgcagcggac gtccggaccg atggcggccc cgaaatgaga cggttctcca
     7681 cgctcgcggc ccttctcctc ctgctcggtg cgcccgccgg tcccctgctg gccggctcgc
     7741 gggaggtgaa gcttcggctc ccgctccgcc cgaagctcgc cgtgacgggc cgggagcggc
     7801 tctccctcgc ccccttcctc gtggcctcga agctctccga gcggaaggag tcgcagcggg
     7861 tcaaggacct cgacctggac ctcgagttcc ggcgctacct cggcaagcag atcgcgcggc
     7921 ggacgaagat gaacgtcgtc acgatgccgg ccgacgtgaa gctgccgtcg accgagctga
     7981 aggcgctcgg cgaggcctcg gcgtactgga aggagctcgg cgcgcgcgtc ggcgccgacg
     8041 tcctcctctc ggggatcgtc gacttccggg tggaggacaa gtcgggctac aagacccagg
     8101 agtaccagag cccgatcgac ggccggacct actaccggca ggacttcgtc gagtcgaccg
     8161 gcttcacgtt cgaggtgacg gtcctcgcgt tcgacgggag gacgggggcg aagctgttcc
     8221 aggagacgtt caaggaccag aaggaaaagt cccggcgggc cgccgacgag atggtcgggc
     8281 tcttcgagaa cctcttctcg ctcgagaacc agctgctcgg cctcttcgtc gtccgggaaa
     8341 gggaggccaa ccgctatgtg ttcgactagg cggcgcgccg cgcggctcgt cccggcgggg
     8401 gccctcgcgg ccctccttct cgtcgcggcc ggcccggctc cggcccaggg gcagaacaag
     8461 gtcgtctacg accgcttcga ctggaagatc tactcctcga cccacttccg catctactac
     8521 tacggcaagg gggaggaggc cctcggcaag gtcgcctcga tggccgagtc ggcctacgac
     8581 gacctctcgc ggcggctgaa ctaccaggtc ccgaagccga ttccgctcct ctactacgtc
     8641 tcccacgcgg agttcgagca gaacaacgtc atcctgaact tcatccccga gggggtcggc
     8701 gcgttcgccg agccggcgcg gaaccggatg gtcctcccga tcgacctccc cgacgacaag
     8761 ctccagaacc tcatcgccca cgagctgacc cacatcttcc agtacgagat cctcttcgga
     8821 ggccggctct cgcgcgccat caccgcgaac atcccgacct ggttcatgga ggggatggcc
     8881 tcgtacttcg cgaaggacga ggacgagaag gaccggatgt tcatccgcga cgcggtgaac
     8941 tccgaccaga tcccgccgat cacgcggatc aacatcaccg gctaccccgc ctaccgcttc
     9001 ggccacgcct tcttcgactt cgtcgaggcc gagtggggga aggacgccgt ccgcgagctc
     9061 gtcttcgagt tccgctcgtt cctcggccgc gacgtcgccg tcgcccttcg ccggacgttc
     9121 gacgtcgagc ccgacgactt cgacctgaag ttccgccgct acctccggcg gaagtacctc
     9181 ccgctcctcg cggtgaaggg agaggcctcg gagtacggcg agcggttccg cgtcggcccc
     9241 tgggagcgcc cctcgtacga ggtgggcgtg gccccgtccc cctcggggga cttcgtggcg
     9301 acgctgacga cgtacaagga agacgtcgac atcgccctcc tctccacgaa gtcgcggaag
     9361 ctctggaaga acctctcccc cggccggacg acgaagtacg agtacatctc ggcgcagttc
     9421 ctcacccagg cgcccgagag cgggcgggac ctctcgttcg cgccggacgg cgaccggatc
     9481 gccgtattcg cccggcgcga gcggggccgc cagctctaca tcttctcggc ccgcggcggg
     9541 gggatcctcg agcggatccc ggtctccccc gacatgcccg tcggcccctc cttctccccg
     9601 gacgggcgcg ccgtcgtctt ctcggggatc tccggcacgt cgcgcgacat cttccggctc
     9661 gacctcgaga cgaaggccgt cacgaacctg acgtcggacg cggcctacga cacggcgccg
     9721 gtctactccc ccgacgggaa gtacctgtac tacacgaagt cgatcggcga gttccagaag
     9781 atcgtccggt tccccgtgga cgacccgaag aaggtcgagc aggtcacgtg gggcgagggg
     9841 aacgacgacg acgcggccct ctcccccgac ggggagcggc tctacttcgc ctcctcccgc
     9901 ggcggcatct tcaacgtcta cgggcaggac ctgaagacgg gcgagatcgt ccagtacacc
     9961 gacgtcatcg gcgcggcgtt cgggcccgcc tcctacatcg ggccggacgg ccaggagaag
    10021 gtcgtcttct ccggctacca ggcgggccgg atccagctct acgtcgccga cgcgaagaag
    10081 ccgttccggc gcctcgagga gaaggccctc ccccccgcgc ccgtcgacgc cgccggcgtc
    10141 cccggcttcg tcccgtccat cgaggtcgcg atcgaccccg agaaggtcga gaagagccgc
    10201 ttcgcgctct tcgtggacga cgtgggcgcc tacgccggcg tccagtccga cggaaccttc
    10261 gtctcctcgg cccggatctc cctctcggac tacctcggcg accgccgcgc cttcgtcatc
    10321 tgggacacgc tcgccggcta ctcgaacgtc aacgcgatct acttcgacct gaagcggcgc
    10381 ttccagtggg ggctgcaggc ctacgacagc cgcatctact tcctcggcta caaccagatc
    10441 ggcgagctga cgtccgaccg gcgcttcctg cgcgagacgg gcgtcaacgt cctcggcgtc
    10501 taccccttct cgcgctatac ccgcgccgag gggtcgctcg gcttcgtctc gcgctcgatc
    10561 gacgccccct acgcctacac gaacgccgac ggcacgcagg ggcagatcgt cctcccgcgg
    10621 aaggacaaca cgatggtggc cgggctcggc ttcggctacg acgcgacgca gttcaaggcc
    10681 ttcggccctc acagcggcca ggctctctcc gtcaactacc gctacctccc cgacttccag
    10741 gacggaggga cgctctcgca ggaggtctcc gccgaggcgc gcctctacct ccccgtcacg
    10801 cgccggtcga acttcgcgat ccgcgcgttc ggcggcgccg cctccggcgc ctcgccggcc
    10861 gtctacgcct tcgggggcct cgacaacctc cgcggctacg actaccgctc cttcatcggc
    10921 aaccggatcg cctacctgaa cgccgagttc cgcttccccc tcatcgacgc cctgatcacc
    10981 gggatcgggc tgaacgtcgg cggcgtccgg ggccgcttct tcttcgacgt cggcggcgcc
    11041 tggctggaag gagaggactt caagtggtgg gacgacgggc gccttcgcga cgggcgcgcg
    11101 gcctgggggg ccggcttcac cgcctacttc ctcgggattc cctggaacgt cgacttcgcc
    11161 cggcccttcg acggacggtc gaacctcgcc ggctggcaga cgaccttcta catcggcccg
    11221 ggctcgttct gagggcgagg gccgggaaag ggtgacccgt gtcaccccgg accggcccgc
    11281 cccgccctag aattcgatcg tgaaggtccg tgtcctcggg gcgtacggag gctcctcgcc
    11341 gtggcatcgc cagacctcct tcctcctgga cgggacggtc gctctcgacg cgggcgcgct
    11401 cacgcagtcc ctcaccctcg acgagcaggc acgcgtccgc tcgatcctcg tcacgcattc
    11461 gcacctcgat cacgtcgcat cgcttccgtt cctcgtggag aacgtcttcg ggaggacgga
    11521 ggggccgatc gagatcatcg ggccgcagga cgtcacctcg ctcctgcagg ggcatctctt
    11581 caacgacgcg atctggcccg acttcacccg gatcccgacg cacttccttc cggccgtgac
    11641 gttccggacg gtcgaggagg ggacgccgtt ccgcgtgaac ggcctgcagg ggctcgcgct
    11701 gtcggtttcg cacgtcgtcc cgacgttcgg ctatctcctc tgggacgacc gctcgtgcgt
    11761 cgtcttctcg ggagacaccg gtccgaccga agccctctgg gccgaggcgc ggcgccgcga
    11821 cgacctgaag gcgatcttcg tcgaatgctc cttccccgat tccatggccg agatcgccga
    11881 ggtttcgagg cacctcacgc cggcgacgct cgcgctcgag atgcgcaagt tcccggccgg
    11941 cgtcccggtg aacctctacc acatgaagcc gcccacgctc ccgcagctcc gggccgacgt
    12001 cgccgcgctc tccgagccgc gggtgcgcat cctcgacgac ggagacgagc tggagttctg
    12061 aaggggtagc gattttctcg gggggaccgc ggcccttcgg gccggcggcc ggtccccccg
    12121 aaccccctcc gcgccggctt gcctctttcg cggtgcccca ccatcaaccc ttcgggccgg
    12181 cggccggtcc ccccgaaccc cctccgcgcc ggcttccgtc ttcgccgcgc ggccctctct
    12241 gcccttcggg ccggcggccg gtccctcagc ccccggcggc gacgcggtcg agggcgagga
    12301 gctcgttcgc aagctcgaag acgaggcggc ggcgggtccc ctcggggagg ccgcgcggcg
    12361 ggaccgggtc gcggagcgtg gcgagcagtc ggagccgggt cccttcgggt cggggctcga
    12421 ggcgaagcgt ccgggagcgg aagagggcga gcggcccctc ggcctcgagg acgagcccgt
    12481 cggggacgac gcggatccgc tcggtccgca ggaggtcgtc tccttcgcgc acccgccagg
    12541 cgaacccgtc ggcgcgcgcc tcgacttcgg tcgcggccca cccttcgggg agcgtgcccg
    12601 cgaacgactc cgggtcggcg aggagcgccc gaagcgcctc gggccgcgcg cccgacgcga
    12661 ggtagaccgc gagggtcggc ggttgcccgg ccgcctcccg cccgtcgtcc ggcggcgcag
    12721 gcgggccgaa gagcgcttcg aggccgtcgg cgtcccgtgc cgggaacggg acccggagcc
    12781 ggctccaggc gtccgagacc tcgaggacgc cgccccgaaa gcgaaggagc cgatcgtcca
    12841 ggaggcggaa gagctccgtc tcttcggagg agcccttcgc cgccgagtcg gagacggagg
    12901 cggggtcgcg gtcgacacgg tagaggagcc gctcctcgcc gcgcgtctcg aggagaacct
    12961 cctctcccgc ggcggcggga cgcacccgga gcgtgccgta tcccgttgcg agctccgcgg
    13021 ccggcggatc gagggagacg gggaggggaa gcgggaacga gacgtcgaag agaagccggc
    13081 cgccgtctgc gacgaggacc gtcctcgggt cgccggcggt gccccgcgcg tgccccgcct
    13141 cgagggcgaa gccggcctcc gccgcgaggg tcgtgaaggc gtccgtcctc gcctcgccgc
    13201 agagccccgc ccccgcctcg agccagcccg cgagccgctc ctcggccgtt ccgcaccccg
    13261 cgcggcggag ggggacctgc gcctggaagc ggacgaagag ctccgcgagg cggcgcggct
    13321 ccgggagcgt cccgccggga tgcccgagcg tttcgagagc ctctcggacg gcgctcatcg
    13381 cgctcgcggc agacccctca ggcggcccgc gccgccgccg cgccgaagac gaggtcgtag
    13441 acgcgcgcgt cggcggcgag ctccggatgg aacgtcgccg cgacgaggtt cccgtcgcgg
    13501 acgaggaccg ggtcgccgtt cctccgggcg agcacctcga cgccggggcc gaggtccgcg
    13561 agcttcggcg cgcggatgaa gacggcctcg agcggctcgc ccccgaggac ggaacggtcg
    13621 aggtcggtga gcgtgacgac ggccgagtcg agctgccggc cgtaggcgtt cctcaccgcc
    13681 gtcgcgtcga ggaggcccag gccgtcccgg tcggggctcg tgatccttcg cgccgagagg
    13741 atcgcgccgg cgcaagtgac gaagagcggg tcgccgcgac gggcgacggc gacgatcgcc
    13801 tcggcgatcc cggtcccctc catcagcttc cacatcgtcg tcgactcccc gcccggcagg
    13861 acgaggcccg cgctcgcgcg gacctcctcc gccgtccgga cgggccgcga gtcgatcccg
    13921 aggcgggcga gggccgcccc gtgcgcctcg aaatcgccct gcagggcgag gacgccgacc
    13981 ggcaccgtca ccagccccgc gtctggagga gctcggcctc gacgagcttg cccgcctcga
    14041 tccccttcat cccctcgacg agcttctccg acgccgccgc gacgcgggcc gggtcgtccc
    14101 agtgcgtcgt cgcctcgacg atcgcgcggg cacggttcgc cgggtcgtcc gacttgaaga
    14161 tcccggagcc gacgaagatc gcctccgcgc cgaggatccg gcagagggcc gcgtcggccg
    14221 gcgtcgcgac gccgcccgcc gcgaagttcg gcaccgggag cttgccgtgc tcggcgaccc
    14281 agacgacgag ctcgaacggc gcggcgagct gcttggcggc ggccatccac tcgtcgcggg
    14341 agaggagcgt gagcgacttc atctgccgcg tcacctcgcg caggtgcctc accgcgtgga
    14401 cgatgtcgcc ggtccccgcc tcgcccttcg tccggatcat cgcggcgcct tcgccgatcc
    14461 gccggagcgc ctcgccgagg ttccggcagc cgcagacgaa gggcaccttg aagtcgtgct
    14521 tgtagacgtg gttctcctcg tcggccggcg tgaggacctc gctctcgtcg atgaagtcga
    14581 cctcgaggga ctccaggacc cgcgcctcgg cgatgtgccc gatccggcac ttggccatga
    14641 ccgggatgga gaccgcggct tcgatctcgc ggatcttcga gaccggcgac atccgggcga
    14701 cgccgccgtc gcggcggatg tccgccggga cccgctcgag cgccatgacg gccgaggcgc
    14761 cggcgtcctc ggcgatcttc gcctgctcgg cgttgacgac gtccatgatg acgcccccct
    14821 tgagcatctc ggcgagaccg accttgaccc ggaaggcgtc ggaggtgaac ggggaggagt
    14881 agatgggggt gctgctcatg gttcgtgtcc ctttcagatc tggatgaggg cttcctcgcg
    14941 ccgctcctcg gcgcgggggg cgcggcggtg cgcccgccgg acggctgccc gggcgaggac
    15001 gccgaggctc tcgagggctt tctcgagctc ggcacccgag ctgcgggcaa cggagatgcg
    15061 gagggagggg acgtcggcgc ggtcgggggc gtagtcgccg ccgggggaga cgaggactcc
    15121 cgcgccgcgc gccgcctcgg cgagctcgcg accggagacg tcctcgggca ggtcgaccca
    15181 gaggacccag cctccgagcg gcctctcgca gcgcgtcccg accgggaagg agcggaggac
    15241 ggtcttgtgg gcgagcgcca ggcgcgcctt cagccgcgcc ttcacccgga ggaggtgccg
    15301 gtcgtagtcg ccgcgggaaa ggaagacgtt caacgcggcc tggaggaccg gcggcgaggt
    15361 gagctcgccg atccgcttca gcgcggcgag gcggtggacg aactcgcggg ggcccgagat
    15421 ccagccgacg cggacgccgg ggaagagcgc cttggagatc gtcccgatgt ggacgacgtg
    15481 ccccggggcg agggcggcga gcggaggcgg aagctccccc tcgacgagga ggtccgcgtc
    15541 gaacgcgtcc tcgacgacga ggacgcccgc cgccgtggcc gccgccacga gctcgcgccg
    15601 gcgggcggcc gtcgcgagga ggccggtcgg gttctggaaa tccgggatcg tgtagaggaa
    15661 cttcggccgc tcggcgagcg cgccgcgaag cggctccatc gccggaccct ccgcgtcgag
    15721 cgggacgccg aggaggcgcg ccccctgcgc ccggaggagg gagagcgcgc ccgaatagct
    15781 cgggctctcc acggcgacgg ggtcgccggg gtcgacgagg gcgcggccga tcaggtcgag
    15841 cccctgctgg gcgccgttca cgatgagcag cgagtcggca tcggccggca cgccgcgcct
    15901 ctcgagacgc gccgcgagcg tctcgcggag cgggcggtag ccgaacggag acccgtacgc
    15961 gaggagcgct ccgtcgcgcg cgacttcggc gaggaccttc gcgaaggctt ccgccgggaa
    16021 ctccttctcc tccggggcga ggcgggccag gtcgtgcgct ccgtcgagcg gtccgtccgg
    16081 cacgccggcc tcggcgagca tccggccgag gagcgccgag gagatgcggt cccaggacgg
    16141 ggccgggccg accatcggcc cggcgagcga ccggcgggcg acgcgggtgc cgcccccctt
    16201 gttcgtctcg atccacccct cgcgcttgag gacctggagc gcctggtgga ccgtggcccg
    16261 gttcacctcg tgggtccggg cgagggcccg cgaggcggga aggcgcgacc cttcctggaa
    16321 ccgccctcgc cggatgtcgc ggatgatccc gtccgcgatc gtccggtaga gggggatgcg
    16381 cccgggggcg ccgtccgcgg cgggaagccg gtccgtcacg tccgaaagga tgaaccttgg
    16441 cctagaccaa ggcaagtatt tcgccgaacg gcaccaacca tttatgggcc gcggcccggc
    16501 cggtcactcc ccccgggcgg cccgctcgtc ccagagccgg cggagctcct cggagaggag
    16561 ctggtcgaac tcccgcgagc cctccggcac ccgccggcct cgcgccagga cccggcgccg
    16621 cgcctcggcc gagagcccgt cgctctcctt ctcccactcc tggagcgccg aggcgatccg
    16681 gttcctcacg aactcggcct ccgccgtcag ctcgacgccc ggagtcttcc ggagcgcctc
    16741 gagcacccgg tgagacaggt cgttggcacg ctcgcgggag atggccattc gccgtactcc
    16801 gtcggagtca atggacgcgg cgcgggccct cgcccgcgtg cgcccggatg gcggcgaccc
    16861 cctgggccgt gagcgggtgg tcgaagaggc ggtcgaggac cgggaacggg atcgcggccg
    16921 catcggcccc catggccgcg gcgtcgagag cgtggaccgg gttctggacc gcgtcgacga
    16981 cgatctgggt ttgcagcccg tagttgtcgt agaccctcac gaccgcgtcg acgacgtcca
    17041 tcccgacctg cccggcgccg tccaggccgg agaccgaaac cgaagcgtag gcggcgccgg
    17101 cccgggcggc caggagcgcc tggagcggcg tcgcgaggag gtggacgtcc gccgcgatct
    17161 gctcgtcgtt caggagcctc acgacgcgaa gcccctcgcg ggtcatcggg agcctcacga
    17221 cgatcgcctt gccgagcttc gcgagctcgc gagcctcctt gtacatcccc ttcggctcgc
    17281 cggccgccac ggcggcgaag acggggccgt cggagacctg ggagatctcg gagaccgcct
    17341 ttcggtagtc gcgccccgac gcaaggaccg catcggcgcg gacgacggcg ccgtcgagca
    17401 gcccccactc cttgacgcgg cgtgcctcgt cggcgtccac ggtgtcgagg aagaacctca
    17461 taccctcact ctactcgctt tccggctcgt ctccggaggg ggtccccggc gctccggcgt
    17521 cctcgtcctc cgcggcctcg tcccgctcgc cgagcgaggc gagcttcgcg acggcgacga
    17581 cggtgtcgcc ctcgtcgagg tcgatcacct tcaccccctg cgccgcgcgg cccgtttcgc
    17641 ggactccggc ggccgcgaag cggatcatca tccccatccg ggtgatgacg aggagctggt
    17701 cgtcctcgcc gaccgacttg atcccgacga cggggccgtt cttctccgtg acgcgcacgt
    17761 tgatgacgcc cgtgccgcca cgcgactggt ggcggtactc ggcgacgtcc gtcctcttgc
    17821 cgtagccgag ctcggtcacg gcgaggaggg tcgcctcctc gtccacgagg tccatctcga
    17881 cgacctcgtc gccctcgcgc agctcgatac ccttcacgcc cgtcgtgtcg cggcccatcg
    17941 gcctcacgtc ggtctccgtg aacttgatcc ccatcccgtt ccgcgtcccg aggaagacct
    18001 ggcgggcgcc gtccgtgacc tggacggaga ggagctcgtc ctcctcctcg aggttgatcg
    18061 cgatgatccc cgcggcgcgg acgttgccgt aggcggagag gacggtcttc ttcacgagcc
    18121 cgcgcttcgt cgcgaagagg aggaagcggt cctccgggaa gccgcgggtc gtcgtcatcg
    18181 cggcgaccgt gtcccccggc tcgacctgga tcaggttcac gagagccttc ccgcgcgccg
    18241 ccgggccggc cgacgggacg tcccagaccc gcacgcagtg gacgcggccc cggtccgtga
    18301 agacgaggag ggagtcgtgc gtcgaggcga cgaagaggtg ctcgacgacg tcctcctcgg
    18361 tcgcggcggc gcccgtgcgc cccttgccgc cccgcttctg ctcgcggtag acggagagag
    18421 gcgtgctctt ggcgtagccg ccccgcgtca ccgtcaggac gaccgcctcg tccttgatga
    18481 ggtcctcgtc ggcgaggtcc ctctcgaggg tcgagatctc cgtccgccgg tcgcatccga
    18541 accgggtccg gatgtcggtc agctccgccc ggatgatcgc ggccacccgc tcgggacggg
    18601 cgaggatgtc cttcaggtcg gcgatgaggg cgaggacgtc cttgtactcg gcgacgatct
    18661 tctcccgctc gaggccggtg aggcgctgga ggcgcatctc gaggatggcc tgcgcctgca
    18721 cctcgtcgag gcggacgagc tcgccgtcgc ggcgggcgcg cgacgacagg tcgagcgccg
    18781 gcaggtcgag gaacgccatc agggcgggga ggtcgacggc gacctcctcc gtgaggcgcc
    18841 gcttcgcctc ctcgggatcc ttcgaccccc ggatgatcgc aatgacgcgg tcgaggttcg
    18901 agagggcgag cgcgaggccg aggaggatgt gggcgcgcgc ctcggccttc gcgaggtcga
    18961 agcgcgtccg gcggacgacg acctggcggc gatggtcgag aaaatgccgg agcatctcct
    19021 tcagcggaag gacgcgtggc tgcccctcga cgatcgcgag gaagatgatt ccgaaggagc
    19081 tctggagcgg cgtcagctgg tagagccggt tcaggaggac gttcgcgttc tcgcccttct
    19141 tcaggtcgac gacgacgcgc attccgtccc ggtcgctctc gtcccggatc gcggagatcc
    19201 cctcgaggcg cttctcgttc acgaggtcgg cgatctgctc gatgaggcgc gccttgttga
    19261 cctggaaggg gatctcggtg atgacgatcg actcgcggtc ggcccgcgtc gaggcctcga
    19321 tctccgcgcg gccgcggacc tggatgatcc cgcggccgct ccggtacgcc tcgacgaccc
    19381 cgcggcggcc gaggaggatt ccgcccgtcg ggaagtcggg cccggggaga aggcggagga
    19441 gctcgtcgag ccccgcgtcg ggcttctcca ggaggaggat cgccgcgtcg cacgcctcgc
    19501 ggaggttgtg cggggggatc ttcgtcgcca tgccgaccgc gatcccctcg gcgccgttca
    19561 cgaggaggtt cgggaacttc gccggaagga cgttcggctc ctggagcgag ccgtcgtagt
    19621 tcggagacca gtcgacggtc tccttgtcga tgtcctcgcc gacgagctcc tcggcgaggg
    19681 ccgtgagccg gacctcggtg tagcgcatcg ccgcggcgct gtcgccgtcg acggagccga
    19741 agttcccctg cccgtcgacg agcggctcgc tcatcgagaa gtcctgggcc atgcgcaccg
    19801 cggtgtcgta gatcgacgcg tcgccgtgcg ggtggtactt gcccatgacg tcgccgacga
    19861 tgcgggccga cttcttgtac gggcggttgt gccggttgcc ctgctcccac atcccgtaaa
    19921 ggacgcggcg gtgaacgggc ttcaggccgt cgcggacgtc ggggagggcg cggcccacga
    19981 tgaccgacat cgcgtagtcg aggtagctgc ggcggatctc ctcctcgatg gagac
//
LOCUS       DAOTEZ010000019        19762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_1800_length_19762_cov_5.515705, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000019 DAOTEZ010000000
VERSION     DAOTEZ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 19762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19762
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1800_length_19762_cov_5.515705"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..2130)
                     /locus_tag="P5164_01825"
     CDS             complement(142..2130)
                     /locus_tag="P5164_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42660.1"
                     /translation="MRKTPIFALLAFLAVVPGLAAGQDIGYDPCPLDAGWTSADGSGD
                     VAVAPDGAGSALSFPTITYQRTTKSVPSSLCASVPTAVSSFASTDAAAYLYFRISGFQ
                     NGEGLSVIWWDPSGEIYHRGSWAPLSTAQYTSYCLEAHISIAGTNAASKPGTWRVQVF
                     SDKIFDVPYVDLTFTVTSSGGTGFPTITNARTAKSVPLNACTPLPTPVTTFAPTDERV
                     YVFFNISGHASSETLTRVWYAPDGSIAASGSWSPLTAGTGYCLAASLTITGTVVATTP
                     GTWRVRVFSDKILDVPYLDLPFTISAGGGSAFPTITNARTTKAVPANQACTPLPVATS
                     SFSSTDARVYLWFQISGHADGERLSRVWYSPDGSVHATGQWDPLPAGGAPCKWAALDI
                     AGASSASKPGTWRVQVFSDRIFDVPYIDKTFTISAGGGGGGGGFTVAGTWLLTSSARV
                     QGAGALWKTDLSIRNKGTSAASVIVKFLGNSGDGRGGPERSVSIPAGQTVTWRDVLSS
                     LFGLTNDYGPILVRASTSDLAILGQTWTAGGAGTYGQSVPVLSTDELIGSTPKSILGV
                     RQDAFFRTNLMLANATESTAAVTVQLVSTAGAVLKTLNVSLGPLSRAQYNVGGDFDYT
                     NLSDAVFVISSPTSGARVGAYASVIDAGTADPRTLLPR"
     gene            2253..2627
                     /locus_tag="P5164_01830"
                     /pseudo
     CDS             2253..2627
                     /locus_tag="P5164_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
     gene            2691..4172
                     /gene="aspA"
                     /locus_tag="P5164_01835"
     CDS             2691..4172
                     /gene="aspA"
                     /locus_tag="P5164_01835"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HRY42661.1"
                     /translation="MNRHLQYQSSRDVGAEKGYASGETRREHDLLGERDVPSDALYGV
                     QTLRAVENFPITGIPVAHFPTLVRALAMVKLAAARANARLGLLPEELADAIIQAAQEV
                     IDGHHHGHFVVDMVQGGAGTSTNMNANEVLANRALEILGRSRGDYASCHPNNHVNLAQ
                     STNDVYPTALRIATILSTQGLVAALERLREALAEKGEEFRDVLKMGRTQLQDAVPMTL
                     GQEFGAFAVTTGEDVARLTETVRLFLEVNMGGTAIGTGINADPRYPALVVEELRNVTG
                     LEVVLSENLVEATPDTGAFVMFSGVLKRVAVKLSKMCNDLRLLSSGPRCGLAEINLPP
                     MQPGSSIMPGKVNPVIPEVVNQVAFQVIGNDLTITLASEAGQLQLNVMEPILAFNLFQ
                     SVDMLTAAIDTLRLRCITGITANREHAREMVERSIGIVTAVVPALGYERSSEVAKEAL
                     ATGRPVRELILEKGYLTAAQLDDLLSIDAMTKPRPLALGDRTV"
     gene            complement(4364..4843)
                     /locus_tag="P5164_01840"
     CDS             complement(4364..4843)
                     /locus_tag="P5164_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRY42662.1"
                     /translation="MITRWNDPFRELESFRSQMNRLFGETFPAARTTEEAPGLAAWAP
                     PVDITETPDQLVFQVELPGFSQDDLKLRAENGVLTLEGERRFEKESEKKAYHRVERAY
                     GRFVRAFSLPANVDPEKISASLVDGVLTVELPKREEAKPKSIPIGIGTAKQISATKK"
     gene            complement(5026..6102)
                     /locus_tag="P5164_01845"
     CDS             complement(5026..6102)
                     /locus_tag="P5164_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HRY42663.1"
                     /translation="MALFEKLALRSITLPNRIGLAPMCQYSAVDGLANDWHLVHLGAR
                     AVGGAGLVMTEAVAVSPEGRISPGDLGLWADGQVGPLARIASFLAAQGAVPAIQLAHA
                     GRKASTKRPWDGGGRALPADGGWEPVFAPSTVPFDEGWQVPEALDADGMARLAAAFAA
                     AARRAADAGFLVAEIHAAHGYLLHEFLSPIANRRGDAFGGSLEHRMRFPLRVVEAVRA
                     AWPTDLPLFLRLSATDWVDGGFVPDEAVAFAREAKARGVDLVDCSSGGMHPQAVVPVA
                     PGYQVPFAARIRKEAEVATAAVGLITEPLQAEEILVSGHADLVLLGRELLRNPHWPLA
                     ASEQLGAAQEGPRQYLRARPYKAL"
     gene            6215..6415
                     /locus_tag="P5164_01850"
     CDS             6215..6415
                     /locus_tag="P5164_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42664.1"
                     /translation="MVKLLLWLLLLVVCWPLAVLALLAWPFLWLLSIPFRIVGALLDG
                     LVGLVRALFLLPARVVGGRARA"
     gene            6448..7161
                     /gene="pepE"
                     /locus_tag="P5164_01855"
     CDS             6448..7161
                     /gene="pepE"
                     /locus_tag="P5164_01855"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HRY42665.1"
                     /translation="MTDARNRLFLVSSSTIHGRGYLDHVADALASFLSGVKTVTFVPY
                     ALADRDGYAAKARERFGALGIELLSVHQAPDPRAAVRAADAVFVGGGNTFRLLKWLQD
                     EGLLGPIRDRVAEGMPYVGSSAGTNVACPSIRTTNDMPIVPTANFLALGLVPYQINPH
                     YLDPDPASTHKGETREERLLQYLEESPVPVVGLREPAWLRVENGVSRLQGDRGARVFK
                     RGAAPFEIEAGAELEAILR"
     gene            complement(7283..7990)
                     /locus_tag="P5164_01860"
     CDS             complement(7283..7990)
                     /locus_tag="P5164_01860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HRY42666.1"
                     /translation="MRLAARPASRAALAALALAAAFLPGCSSRPPARGAPDLAGEGTE
                     RGLASWYGAEFAGLPTASGEIFDPTRISAAHRALPLGTIVDVTNEKNGRTVRVRVNDR
                     GPFVAGRIVDLSRAAAAEIEAVADGVVPVTLTVVSLGSGRRTRSGKPGPGDAATSWAV
                     QAGAFANAENAERLKARLAARYPSPWLEPYQGLTRVKLGPYRSRDEADEACAGLADFG
                     LAGIVVPHGTGEAESGR"
     gene            complement(7987..9429)
                     /gene="purB"
                     /locus_tag="P5164_01865"
     CDS             complement(7987..9429)
                     /gene="purB"
                     /locus_tag="P5164_01865"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013112074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRY42667.1"
                     /translation="MNADAYENPLTARYASAEMSALFSARHKFATWRRLWLALAEGER
                     ELGLPIPEAAIAALAGRLVPTDEELAAAERYERETKHDVMAQIHALGDAVPEARAFLH
                     LGATSAFVGDNADLLVLRDALSLLERRVVRVISRLAAFAREHADLACTGWTHFQAAQP
                     TTVGKRACLWLQDLVLDHHELERRAAELRFLGCKGATGTQASFVALFGGDAEKADRLD
                     RRVAEKMGFPKRLLVSGQTYTRKQDALVLSALSGLASSASKFAHDLRLLQHLKEVEEP
                     FGAKQVGSSAMPYKRNPMKCERMNALARWILTTAENGSWTHATQWLERTLDDSANRRL
                     ALAESFLAADALLVLQEAVVDGLVVHPEVIRRRLAEELPFLATENVLMAAAKKGGDRQ
                     VLHERIRVHAQAAGDRLKAAGGENDLLERIAADPAFGLSREEVAAAADPRLFVGRAPA
                     QVAEFLAAEVDPLLAARAEALAGRAVEVKV"
     gene            complement(9426..9596)
                     /locus_tag="P5164_01870"
     CDS             complement(9426..9596)
                     /locus_tag="P5164_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42668.1"
                     /translation="MSEDVEFIICNNCETPCYSFELDRKGNVATAYCALCGADEAKDF
                     RLPEAEDVEDQG"
     gene            9645..10928
                     /locus_tag="P5164_01875"
     CDS             9645..10928
                     /locus_tag="P5164_01875"
                     /inference="COORDINATES: protein motif:HMM:NF014344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HRY42669.1"
                     /translation="MPIAVHSEIGRLRSVVVHQPGREIDRMTPGMMQDLLFDDILHGA
                     RAREEHRRFQQVLRLFAEVLEIQDLLEEVLADADRRVQVLADLDEALRLGPAVTDRLS
                     ALPPELLAAALVEGIEQPHPEITGSIDSLFLLPPISNYYFQRDPVIPVGDRLVRGSMA
                     TPARLREPLVSGAVFEHHPRFARPDGVFWFHEFRAAWGKNVSYARMRPHIEGGDVLVL
                     REDLLAIGYSERTEETTIERLSEALKKAGSAVRHIVVVALPHERSCMHLDTVFTQVSE
                     GECLCYSPMILAGGAEEADVYSVDLTHDEITWTPEHDFLSALKRRGLDLEPIPCGGRD
                     PIDEQREQWTDGANAFAVAPGVIVCYDRNEKTAEELDRHGYEIVRDEDLLLGRREIDP
                     NGTKKVAIQLSTTELARARGGPRCMTMPLVRERLG"
     gene            10934..11884
                     /locus_tag="P5164_01880"
     CDS             10934..11884
                     /locus_tag="P5164_01880"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRY42670.1"
                     /translation="MRAPLGAVALLAALLAGCGGKTPPAEPPAKVEGSAALVSLSRVP
                     LRAEASAKGATVALLRRGAEVKVEASEGAFARVTTKDGRTGWIESGSWEMASEKAARE
                     RRAEAVKRFETVPGRIVAPCPVYLAPDFGAARWGELEDGDPVEVVLADHDFLGVRLLG
                     IDLAFVPAHAVRYVAPDVPTPPVPAPAAPEAAAPGPGGPVDVPAGGFGAETAADASAE
                     PYTALPFGATPPVLRTRVEPAYPAAARKAGLGGDVVLQVVVERDGTIGSVFAVQEGPL
                     GLTTAATDAVRRWVYEPARLDGRPIAVMKTVRVRFQAAAP"
     gene            11931..13568
                     /locus_tag="P5164_01885"
     CDS             11931..13568
                     /locus_tag="P5164_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY42671.1"
                     /translation="MYELVYEVEGRPQRFRLTGTEVFLGRSSENEIVLNDFSVSRRHA
                     VIRQEDGTWVVIDQNSTNGVKVNGKFVASSPIVPGDVLTVGTFSLTVREEAPAVRPLP
                     PVPVVGESSSTFIRSIADFNRDFHLEDYVPEEEGDALSASGKRSTSAVARSKIFEILV
                     QVAKTLILAREVHEVLGRVVDLLFEHLPVDRAVVVLVGDEGGLVPMLARQRGKEPGEA
                     EEIFSHTIVESVVKNRVAVLTSDALADGRFEAGQSIRIQQIRSAMCVPLWNQDRVIGA
                     LHVDTPLRVGSFTADDLDLLTALGNFAAVAIERARLQRRIEREERIRERLSRYHSPGV
                     VEEIASDASGVIERVRTRNVTVFFADIVGFTSAAEGMEPEALSRFLSTFFTYAADAIF
                     AQGGTLDKFIGDAVMAFFGAPIVQPDHARRAVAAAAKLVENVKAWNLERVARGEPPVG
                     VRIGVNTGNVVVGDIGSAQRVDYTVLGNTVNVAARIEEYVAGPNEVAVGEETARLTSD
                     DFSYEPLGDVKLKGLSRGLPCYRVKLDARGVPLPAPR"
     gene            13569..14165
                     /locus_tag="P5164_01890"
     CDS             13569..14165
                     /locus_tag="P5164_01890"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HRY42672.1"
                     /translation="MPPSLENLVFLTSNAGKAREASVLLGRPVEARALDLPEIQSLDF
                     VEVVTAKALAAALQLGVPVLVEDSGLALPGWKGYPGPLTKYAVGAVGEAGFARLVHAW
                     GDPRAVAVSTLGLAWPGAAPAEVVVAVGRVPGSLAAEPRGASGFGWDVIFVPDGETRT
                     FAQMSAEEKNARSHRAKAFAALVERLGISSRGGGGVTS"
     gene            14162..14812
                     /locus_tag="P5164_01895"
     CDS             14162..14812
                     /locus_tag="P5164_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42673.1"
                     /translation="MIELLGNPWFAATFLVFAAAVLVFIARRNRTREEAAKGPSRSSQ
                     RARTEPAARSAAFAAVPAPESRSPGASLDAASLPPGFRDLTWGSAPVDGMQLVAENGD
                     HRFLSRPTDSLRIGRAVLTSITYVYERGRLQATVIELPASGFELLQRQLREDWGAPRT
                     LKPDQKYAWADMRASDEATQAILEKPPASRTAKLVVSAKAVSTARTRDAGAADAGA"
     gene            14953..15333
                     /locus_tag="P5164_01900"
     CDS             14953..15333
                     /locus_tag="P5164_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42674.1"
                     /translation="MGALLFIVSWGIVIVGGLPKRTQEALEFRFGWWSAKVAGLLSAA
                     LELHAGYRLVRVTLAAFSMERVSVTAAAILYAISAFLLLEGLIRFLVTYRMGDLALPS
                     LPVWAVARLIEWLRPSDRSSSATA"
     gene            15461..15652
                     /locus_tag="P5164_01905"
     CDS             15461..15652
                     /locus_tag="P5164_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42675.1"
                     /translation="MPLTNLRAGSEELDFFVPTASGKYEGPTMKLFLQLVPKAELKDY
                     AQGRKLRRVERSPDFCSGP"
     gene            complement(15671..16288)
                     /gene="coaE"
                     /locus_tag="P5164_01910"
     CDS             complement(15671..16288)
                     /gene="coaE"
                     /locus_tag="P5164_01910"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRY42676.1"
                     /translation="MLRVGLTGGIASGKSAVGSRWRALGIPVLDADRLVHALYEPGAP
                     GAAAVAEEFGAELLDERGAVDRPRLARLAFAEPATVARLNARIHPIVRVETERWLAER
                     ESEGHPVAVVEATLLVENNGRDRYDVLVAVSAPEELRLARALARDPGATRDTVLARMR
                     AQLPDAERNAACDEVIVTDGTLEELSTKAEAVAARLRSRASGTRA"
     gene            complement(16291..17211)
                     /locus_tag="P5164_01915"
     CDS             complement(16291..17211)
                     /locus_tag="P5164_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HRY42677.1"
                     /translation="MTATILDGARVAADIRRETAEAASELAARGAVPGLAVILAGDDP
                     ASQVYVRNKEKGAREAGIRAETLRFPATVTEAMLLAEIGRLNADPEVDAILVQLPLPA
                     GIGSSRILEAVDPAKDVDGFHPFNVGRLVQGKPAPVPCTPAGVMELLRRENVPLRGTR
                     AVVLGRSDIVGKPMATLLTKADATVTVAHSKTRDLPALCREADLLVAAIGRPAFVTAD
                     FIREGAVVVDVGINRIDTAEEVARCFPGNEAKAAAFAAKGSLLVGDVHPVDVTERASR
                     YTPVPGGVGPLTIARLLANTLDLCRARRRL"
     gene            complement(17208..17879)
                     /locus_tag="P5164_01920"
     CDS             complement(17208..17879)
                     /locus_tag="P5164_01920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014630322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY42678.1"
                     /translation="MFSRIAPRYDLLNRLLSGGTDVVWRNEAVRLLAPRPGERILDLC
                     SGTGDLALTVARRSPGVRVVAADFTFEMLVLGRAKFLRRGARIPEAGADGLSLPFPDR
                     TFDGATAAFGVRNFEDLDRGLRELHRVLVPGGRLVVLEFVPEPTGPLAPLVKFHVRRF
                     VPFLGRLVSKDSSAYQYLPDSVGKWPAPDALSGRMREAGFSSVEVRLLSFGIAAAHVA
                     RKGAS"
     gene            18230..18637
                     /gene="rplM"
                     /locus_tag="P5164_01925"
     CDS             18230..18637
                     /gene="rplM"
                     /locus_tag="P5164_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011317578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRY42679.1"
                     /translation="MNELPRRWIVVDADGKVLGRLATVVAMHLTGKSKPTYTPFLDTG
                     DFVVVVNAEKVALTGVKETAKLYRYHTGYPGGLKTTSADKLRATRPEKIVEEAVRGML
                     PKTKLGKKMFKKLKVYAGPSHPHVAQVPKPVSA"
     gene            18654..19046
                     /gene="rpsI"
                     /locus_tag="P5164_01930"
     CDS             18654..19046
                     /gene="rpsI"
                     /locus_tag="P5164_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRY42680.1"
                     /translation="MSNLQYHAIGRRKEAVARVYLRPGSGAFVVNDRPFEKYFPNDVL
                     KMVIRQPLQLTESTEKFDIVARVAGGGSAGQAGAIRHGLARALCLFNLELRPSLKKAG
                     LLVRDSRMKERKKYGQRGARARFQFSKR"
     gene            19049..>19762
                     /gene="rpsB"
                     /locus_tag="P5164_01935"
     CDS             19049..>19762
                     /gene="rpsB"
                     /locus_tag="P5164_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRY42681.1"
                     /translation="MSQITMKELLEAGVHFGHQTKRWNPKMKKYIFGKRNGIYIIDLQ
                     KTLKCFREAAAFVTEAASQGKNILFVGTKRQAQDAIYEEANRCGMFFVNNRWLGGTLT
                     NFVTIRKSIMRLKEIEGMLAENSEAILTKKERIRLERERTKLAKNLSGITEMEELPDL
                     LFVIDPKKEAIAVQEANKLGIPIVGIVDTNCDPDPITHVIPGNDDAIRAIKLFTGKIA
                     DAVLEGLHAQEERFVGKAAE"
BASE COUNT         2820 a         6939 c         7195 g         2808 t
ORIGIN      
        1 cgaagatgtg ggaggcgacg gggctgccgc tcccgcagct cctcgaccgg ctcgtcgcga
       61 gcgcgttccg caggccgctc cgggggcgct gaggccggga cccgcggagg cgcggacccc
      121 ggccgtcatg gtcccggccg ctcagcgcgg cagcagcgtc cgcgggtccg ccgtccccgc
      181 gtcgatgacg gaagcgtagg cgccgacgcg cgccccggaa gtcggcgagg agatgacgaa
      241 gaccgcgtcg gagaggttcg tgtagtcgaa gtcgccgccc acgttgtact gggcgcgcga
      301 gagcggcccg agggagacgt tcagcgtctt gaggacggcc cccgcggtcg agacgagctg
      361 gacggtgacc gcggcggtcg actcggtcgc gttcgcgagc atcaggttcg tccggaagaa
      421 ggcgtcctgg cggacgccga ggatcgactt cggcgtgctc ccgatcagct cgtcggtcga
      481 gaggacgggg acgctctggc cgtacgtccc ggcgccgccc gcggtccagg tctggccgag
      541 gatcgcgagg tccgacgtcg aggcgcggac gaggatcggc ccgtagtcgt tcgtgaggcc
      601 gaagaggctc gagaggacgt ctcgccacgt gacggtctgg ccggcaggga tcgagacgga
      661 ccgctccggc ccgccgcgac cgtcgcccga atttccgagg aacttcacga tcaccgaagc
      721 cgccgacgtc cccttgttcc ggatcgagag gtcggtcttc cagagggccc cggcgccctg
      781 gacgcgggcg ctcgaggtga ggagccaggt gccggcgacg gtgaatcctc ctcctccgcc
      841 cccgccgcct gcggagatcg tgaacgtctt gtcgatgtac gggacgtcga agatccggtc
      901 ggagaagacc tggacgcgcc acgtccccgg cttcgacgcc gacgacgcgc cggcgatgtc
      961 cagcgcggcc cacttgcacg gcgcgcctcc cgcaggcaga gggtcccact ggcccgtcgc
     1021 gtggacactt ccgtcgggcg agtaccagac gcgcgagagc cgctcgccgt ccgcgtgccc
     1081 gctgatctgg aaccagaggt agacgcgcgc gtcggtcgag gagaacgagg aggtggcgac
     1141 ggggagcggc gtgcacgcct ggttcgccgg caccgccttc gtcgttcgcg cgttcgtgat
     1201 cgtcgggaag gccgagccgc ctcccgcgga gatcgtgaac ggcaggtcga ggtacgggac
     1261 gtccaggatc ttgtcggaga agacccgcac gcgccacgtc cccggcgtcg tcgcgacgac
     1321 ggtgccggtg atcgtcaggc tcgcggccag gcagtagccc gtgccggccg tcagcgggct
     1381 ccagctcccg gaagcggcga tggaaccgtc cggcgcgtac cagacgcgcg tcagcgtctc
     1441 gctgctcgcg tggccgctga tgttgaagaa gacgtagacc ctctcgtccg tcggggcgaa
     1501 cgtcgtcacc ggcgtcggca gcggcgtgca ggcgttcagg gggaccgact tcgccgtccg
     1561 cgcgttcgtg atcgtcggga agccggtgcc gccgctcgag gtgacggtga acgtcaggtc
     1621 gacgtaggga acgtcgaaga tcttgtcgga gaagacctgg actcgccagg tgccgggctt
     1681 ggaggcggcg ttcgtccccg cgatggagat gtgggcctcg aggcagtagg acgtgtactg
     1741 cgccgtcgag aggggagccc aggagccccg gtggtagatc tcgccgctcg ggtcccacca
     1801 gatgaccgaa agcccttcgc cgttctggaa gccgctgatc cggaagtaga ggtaggcggc
     1861 cgcgtcggtc gaggcgaagg agctcacggc cgtcggcacg gacgcgcaga ggctcgacgg
     1921 gaccgacttc gtcgtcctct ggtaggtgat cgtcgggaac gagagcgcgg agccggcccc
     1981 gtcgggggcc acggcgacgt ctccggagcc gtccgcggac gtccagccgg cgtcgagcgg
     2041 gcacgggtcg tatccgatgt cctgaccggc cgcgagaccc gggacgacgg cgagaaacgc
     2101 gagcagagcg aagatcggcg tctttctcat cgtctcctcc atcagggacg agacgcgaga
     2161 ccacggctcc ggcgggcgcg gaggggccgc ggtcccgcgt cgctcctccg tcggccattc
     2221 tacgctgcgg cgcggcaggc gccctcacta caatgggagc tcgccgccgt gacggcggtg
     2281 cagggaggtg tctcgtgaac caggttcgtg cggcgctcgg ggcgacggag atcttctcgg
     2341 gcctttcgga gagggtcctc gacctcctcg cgggggcggc gatccgccgc gacgtgccgg
     2401 ccgagacgat gctctggacg cagggcgcgc cgcgccgcgc cctcttcgtc atcctcgacg
     2461 ggagcgtcga gatctcgcgc gagaaggacg gggcaatcga gccgctctcg gtcgaggggc
     2521 gcggcgaggc ggtcggcgtc ggggcgctcc tccatacctc cgagcacacc acgtcggccc
     2581 gggcgctgac ggacgtcacg gtcatcgagc tgccgcgcgc ggaggtccgg gcgcggctca
     2641 tgggcgacgc gggcgcggcg atggacgtcg tctcgcgcgt ggcgatcgtc atgaaccggc
     2701 acctgcagta ccagagctcg cgggacgtcg gcgcggagaa ggggtacgcg tcgggagaga
     2761 cccgccgcga gcacgacctc ctcggcgaac gcgacgtccc ctccgacgcc ctctacggag
     2821 tccagacgct ccgcgccgtc gagaacttcc cgatcaccgg catcccggtc gcccacttcc
     2881 cgacgctcgt tcgcgccctc gcgatggtga agctcgccgc cgcgcgcgcg aacgcccgcc
     2941 tcggcctcct gccggaggag ctggcggacg cgatcatcca ggcggcgcag gaggtgatcg
     3001 acggtcacca ccacgggcac ttcgtcgtcg acatggtcca ggggggcgcc ggcacgtcga
     3061 cgaacatgaa cgcgaacgag gtcctcgcga accgcgcgct cgagatcctc ggccgctccc
     3121 gcggcgatta cgcctcctgc cacccgaaca accacgtcaa cctcgcccag tcgacgaacg
     3181 acgtctaccc gacggcgctc cggatcgcca cgatcctctc gacgcagggg ctggtcgccg
     3241 ccctcgaacg gctccgcgaa gcgctcgccg agaaggggga ggagttccgt gacgtcctga
     3301 agatggggcg gacgcagctg caggacgccg ttccgatgac gctcgggcag gagttcggcg
     3361 cgttcgcggt gacgacgggg gaggacgtcg cccggctcac ggagacggtc cggctcttcc
     3421 tcgaggtgaa catgggcggc acggcgatcg gcaccgggat caacgccgac ccgcgctacc
     3481 cggccctcgt cgtggaggag ctccggaacg tcaccggcct cgaggtcgtc ctctcggaga
     3541 acctcgtgga ggcgactccc gacaccggag cgttcgtcat gttctccggg gtcctcaagc
     3601 gcgtcgccgt gaagctctcg aagatgtgca acgacctccg gctcctctcg tcggggccgc
     3661 gctgcggcct cgccgagatc aacctcccgc cgatgcagcc cgggtcgagc atcatgccgg
     3721 ggaaggtgaa cccggtcatc cccgaggtcg tcaaccaggt cgcgttccag gtcatcggca
     3781 acgacctgac gatcacgctc gcctccgagg cggggcagct ccagctgaac gtcatggagc
     3841 cgatcctcgc gttcaacctc ttccagtcgg tcgacatgct gaccgcggcg atcgacacgc
     3901 tccggctccg ctgcatcacc gggatcacgg cgaaccgcga gcatgcgcgg gagatggtgg
     3961 agcgctcgat cgggatcgtg acggcggtcg tcccggccct cggctacgag cggtcgagcg
     4021 aggtggcgaa ggaggcgctc gccacggggc ggcccgtccg ggagctgatc ctcgagaagg
     4081 gctacctcac cgcggcccag ctcgacgacc tcctctcgat cgacgcgatg acgaaaccgc
     4141 ggccgctcgc cctcggcgac cggaccgtct gaggctggcg gccgggctcg cgccggggcg
     4201 gttcgttcgc cctcggcgcg ggctcgagtc ggcgccggcg aggacgggga ggccggcagc
     4261 gcgcgtcggt gcggacgggg cggccaccgg gagataacgg acacggcggg cctcgcggcc
     4321 cgccgtgtgg cgatcgtcgg ggccggcgtc ccggcgccag cggttacttc ttcgtggcgg
     4381 agatctgctt cgccgtgccg attccgatcg ggatcgactt gggcttcgcc tcttcccgct
     4441 tcgggagctc gacggtcagg acgccgtcga cgaggctcgc ggagatcttc tccgggtcga
     4501 cgttcgcggg gagggagaag gcccggacga agcggccgta cgccctctcg acgcggtgat
     4561 acgccttctt ctcgctctcc ttctcgaacc tgcgctcgcc ctcgagcgtc aggaccccgt
     4621 tctcggcgcg gagcttgagg tcgtcctgcg agaagcccgg gagctcgacc tggaagacga
     4681 gctggtccgg cgtctcggtg atgtcgaccg gcggcgccca ggcggcgagt cccggcgcct
     4741 cctccgtggt ccgggcggcg gggaacgtct cgccgaagag gcggttcatc tggctgcgga
     4801 aggactcgag ctcgcggaag ggatcgttcc aacgggtgat catgatgctt acctccctct
     4861 gaagattcga tttctggtcc gtgggctcca tttcttcatt ggaggcctca ggtccggagt
     4921 aaatatagaa tctgattgcc agattgtcaa gtatcagggt gcgaagatcc tctcggtgtc
     4981 ggcccgctcg gtcgttccct cggattacac gtagaagtta tttgtttaga gtgctttgta
     5041 tggcctggcc cgtaggtact gtcgcggtcc ctcctgcgcg gcgccgagct gctccgaggc
     5101 cgcaagcggc caatgcgggt tgcgcaggag ctcccggccg agcagcacga ggtctgcatg
     5161 gccggaaacg aggatctcct cggcctgaag cggctcggtg atgagaccaa cggccgcggt
     5221 agcgacctca gcctcctttc ggatccgagc cgcgaacggg acctggtatc cgggtgcgac
     5281 cgggacgacg gcctgcgggt gcattccgcc cgaggagcag tcgacgaggt ccacgccccg
     5341 ggcttttgcc tcgcgggcga aggcgaccgc ctcgtccggg acgaagccgc cgtcgaccca
     5401 gtcggtcgcg gagagccgca ggaaaagggg caggtcggtc ggccaggcgg cgcggaccgc
     5461 ttcgacgacg cgaagcggga agcgcatcct gtgctcgagg gagccgccga aggcatcgcc
     5521 cctccggttc gcgatcggcg agaggaattc gtggaggaga tagccgtgcg cagcgtggat
     5581 ctcggcgacg aggaacccgg cgtcggccgc gcgtcgggcg gcggcggcga aggcggcggc
     5641 gaggcgggcc atcccgtcgg cgtcgagagc ctcaggaacc tgccaccctt cgtcgaacgg
     5701 cacggtgctc ggtgcgaaga ccggctccca cccgccgtcc gccgggagcg cacggcctcc
     5761 accatcccag gggcgcttcg tggaggcctt gcgtccggcg tgcgcgagct ggatggccgg
     5821 tacggctccc tgcgccgcga ggaaggatgc gatgcgcgcg agcggcccga cctgcccgtc
     5881 ggcccagagg ccgagatcgc cggggctgat ccgtccttcg ggcgagacgg cgaccgcctc
     5941 ggtcatgacg aggccggcgc cgccgacggc gcgcgcgccg aggtggacga ggtgccagtc
     6001 gttcgcgagg ccgtcgacgg cggagtactg gcacatgggg gcgaggccga tccggttcgg
     6061 aagggtgatc gatcgaaggg cgagcttctc gaagagcgcc atgcccgaag cctacggcaa
     6121 ggacggcctg ctccgacgga taggccgacg ggtcgcggca gatcgccgaa gccgccgcga
     6181 acccgcgcgt cccggcgccg tatcagggag ggagatggtc aagctcctcc tgtggctcct
     6241 cctcctcgtc gtctgctggc cgctcgcggt cctcgcgctc ctcgcgtggc ccttcctctg
     6301 gctcctgtcg atcccgttcc ggatcgtggg cgcgctcctg gacggcctcg tcgggctcgt
     6361 ccgggcgctc ttcctcctcc cggcgcgcgt cgtcggcggg cgggcccggg cctgacgggg
     6421 agcctcgcgg caggctaacc tccggacatg accgacgcgc gaaaccggct cttcctcgtc
     6481 tcgagctcca cgatccacgg ccgcggctac ctcgaccacg tcgcggacgc cctcgcctcg
     6541 ttcctctcgg gcgtgaagac ggtgacgttc gtcccgtacg ccctcgccga ccgcgacggc
     6601 tacgccgcga aggcgcgcga gcgcttcgga gcgctcggaa tcgagctcct gagcgtccac
     6661 caggccccgg acccgcgcgc cgccgtccgc gccgccgacg cggtcttcgt cggcggcgga
     6721 aacaccttcc ggctcctgaa gtggctccag gacgaaggcc tcctcggccc gatccgggac
     6781 cgcgtcgccg aggggatgcc ctacgtcggc tcgagcgcgg ggacgaacgt cgcgtgcccg
     6841 tcgatccgga cgacgaacga catgccgatc gtcccgacgg cgaacttcct cgcgctcggt
     6901 ctcgtcccct accagatcaa cccgcactac ctcgaccccg acccggcctc cacgcacaag
     6961 ggggagacgc gcgaggaacg gctcctccag tacctcgagg agagcccggt gccggtggtc
     7021 ggccttcgcg agccggcgtg gctccgcgtc gagaacggcg tctcccgcct ccagggcgac
     7081 cgcggagccc gcgtcttcaa gcgcggcgcg gcgccgtttg agatcgaggc gggcgcggag
     7141 ctcgaggcga tcctccgata ggaggtcctg gcccgggggc ctcgaggacc ctcccgcgcc
     7201 ggatgcgtcg tatcccgtgt cacaggcggc ggaccggttc cgaccgcggg agcctcgggc
     7261 gaaccggggg tttcgccggc tcctatcggc ctgattcggc ctcgccggtc ccgtgcggga
     7321 cgacgatccc ggcgaggccg aagtcggcca ggccggcgca ggcctcgtcc gcctcgtcgc
     7381 gcgagcgata cggcccgagc ttgacgcgcg tcagcccctg gtacggctcc agccagggcg
     7441 acgggtagcg cgccgcgagc cgcgccttca gccgctcggc gttctccgcg ttcgcgaagg
     7501 ccccggcctg gaccgcccag gaggtcgcgg cgtcgcccgg gccgggcttc ccggaccgcg
     7561 tccgccggcc cgacccgaga gagacgaccg tgagcgtcac cgggacgacg ccgtcggcga
     7621 ccgcctcgat ctcggccgcg gcggcgcgcg agaggtcgac gattcgcccg gcgacgaacg
     7681 gaccgcggtc gttgaccctc acgcggacgg tccggccgtt cttctcgttc gtcacgtcga
     7741 cgatcgtgcc gagcgggagg gcgcggtgcg ctgcggagat ccgcgtcggg tcgaagatct
     7801 cgccgctcgc cgtcgggagg ccggcgaact cggctccgta ccaggaggcg agcccgcgct
     7861 ccgtcccctc gccggcgagg tcgggcgctc cccgcgccgg cgggcgggac gagcagccgg
     7921 gaaggaacgc ggcggcgagc gccagcgccg cgagcgccgc gcgcgacgcc ggccgcgccg
     7981 cgagcctcag accttcacct cgaccgcccg gcccgccagc gcctcggcgc gcgccgcgag
     8041 gagcgggtcg acctcggccg cgaggaactc cgcgacctgc gcgggcgcgc ggccgacgaa
     8101 caggcgcggg tcggccgccg cggcgacctc ctcgcgggaa aggccgaaag ccgggtcggc
     8161 ggcgatccgc tcgaggaggt cgttctcgcc tcccgcggcc ttcaggcggt cgcccgcggc
     8221 ctgcgcgtgg acgcggatcc gttcgtggag cacctgccgg tcgcccccct tcttcgcggc
     8281 cgccatcagg acgttctcgg tggcgaggaa cgggagctcc tcggcgaggc ggcgtcggat
     8341 cacctcgggg tggacgacga gcccgtcgac gaccgcttcc tggaggacga ggagggcgtc
     8401 ggcggcgagg aacgactcgg cgagggcgag gcggcggttc gcggaatcgt cgagcgtccg
     8461 ctcgagccac tgcgtggcgt gggtccagga gccgttctcg gccgtcgtca ggatccagcg
     8521 cgcgagggcg ttcatccgct cgcacttcat cgggttccgc ttgtacggca tcgccgagga
     8581 gccgacctgc ttcgcgccga acggctcctc gacctccttc aggtgctgga ggaggcggag
     8641 gtcgtgcgcg aacttcgaag ccgaggaggc gaggccggag agggccgaga ggacgagagc
     8701 gtcctgcttg cgagtgtagg tctggccgga gacgaggagc cgcttcggga agcccatctt
     8761 ctcggccacc ctccggtcga gccgatcggc tttctccgcg tcgccgccga aaagagcgac
     8821 gaacgaagcc tgcgtcccgg tcgccccctt gcatccgagg aagcggagct cggcggcgcg
     8881 gcgctccagc tcgtggtggt cgaggacgag gtcctggagc cagaggcagg cgcgcttgcc
     8941 gaccgtcgtc ggctgcgcgg cctggaagtg ggtccagccc gtacaggcga ggtccgcgtg
     9001 ctcgcgggcg aacgcggcga ggcgcgagat cacgcggacg acgcgccgct cgagcagcga
     9061 gagcgcgtcg cggaggacga ggaggtcggc gttgtccccc acgaaggccg aggtcgcccc
     9121 gaggtggagg aaggcgcgcg cctcggggac ggcgtccccg agcgcgtgga tctgcgccat
     9181 gacgtcgtgc ttggtctcgc gctcgtaccg ctcggccgcg gcgagctcct cgtccgtcgg
     9241 gacgaggcgg cccgcgagcg cggcgatcgc cgcctcgggg atcggcagtc ccagctcgcg
     9301 ctcgccctcg gcgagcgcga gccagagccg gcgccacgtc gcgaacttgt gccgcgccga
     9361 gaagagcgcg gacatctcgg ccgacgcgta gcgcgcggtg agggggttct cgtacgcgtc
     9421 cgcgttcaac cctggtcctc gacgtcctcg gcttcgggaa gtcggaagtc cttcgcctcg
     9481 tcggcgccgc agagggcaca gtacgcggtg gcgacgttcc ccttccggtc cagctcgaac
     9541 gagtagcagg gcgtctcgca gttgttgcag atgatgaact ccacgtcctc gctcatcttc
     9601 gtctccgctt ggtattctcc cgcggctgcc acgggggtgc caccatgccg atcgccgtcc
     9661 attccgaaat cggtcgtctg cgttccgtcg tcgtccacca gcccggccgc gagatcgacc
     9721 ggatgacccc ggggatgatg caggatctcc tcttcgacga catcctgcac ggcgcgcggg
     9781 cgcgcgagga gcaccggcgg ttccagcagg tgctgcggct tttcgccgag gtcctcgaga
     9841 tccaggacct cctcgaggag gtcctcgccg acgccgaccg gagggtccag gtcctcgcgg
     9901 acctcgacga ggccctccgc ctcggcccgg ccgtcacgga ccgcctctcc gcgctcccgc
     9961 ccgagctcct cgcggcggcc ctcgtcgagg ggatcgagca gccgcacccc gagatcaccg
    10021 gctcgatcga ctcgctcttc ctcctgccgc cgatctcgaa ttactacttc cagcgagacc
    10081 cggtcatccc ggtgggcgac cggctcgtcc ggggctccat ggcgacgccg gcgcgcctgc
    10141 gcgagccgct cgtctccggc gccgtcttcg agcaccaccc gcgcttcgcc cgccccgacg
    10201 gcgtcttctg gttccacgag ttccgcgcgg cctgggggaa gaacgtctcc tacgcgcgga
    10261 tgaggcccca catcgaaggg ggcgacgtcc tcgtcctccg cgaggacctc ctcgcgatcg
    10321 gctactccga gcggaccgag gagacgacga tcgagcgcct ctcggaggct ctgaagaagg
    10381 ccggctcggc ggtgcggcac atcgtcgtcg tcgccctccc gcacgagcgc tcctgcatgc
    10441 acctcgacac ggtcttcacg caggtctccg aaggggagtg cctctgctac tccccgatga
    10501 tcctcgccgg cggggcggag gaggccgacg tctactcggt cgacctgacg cacgacgaga
    10561 tcacctggac gccggagcac gacttcctct cggccctgaa gcgccgcggc ctcgacctcg
    10621 agccgatccc gtgcgggggc cgcgacccga tcgacgagca gagggagcag tggaccgacg
    10681 gggcgaacgc gttcgccgtc gcgccggggg tcatcgtctg ctatgaccgg aacgagaaga
    10741 cggcggagga gctcgaccgg cacgggtacg agatcgtgcg ggacgaggac ctcctcctcg
    10801 gccggcgcga gatcgacccg aacgggacga agaaggtcgc gatccagctc tccacgaccg
    10861 agctcgcgcg ggctcgcggc ggcccgcgct gcatgacgat gcccctcgtg agggagcggc
    10921 tgggctgagc cgcgtgcgcg cccccctcgg ggccgtggcc ctcctcgcgg cgcttctcgc
    10981 cgggtgcgga gggaagacgc ctccggcgga gccgccggcg aaggtggagg gctccgccgc
    11041 gctcgtctcg ctctcccgcg tcccgcttcg cgccgaggcc tcggcgaagg gggcgaccgt
    11101 ggcgctcctt cggcgcggcg cggaggtgaa ggtcgaggcg tccgaagggg cgttcgcccg
    11161 cgtcacgacg aaagacggcc ggaccggctg gatcgagtcg gggagctggg agatggcgag
    11221 cgagaaggcc gcgcgcgagc ggcgggcgga ggccgtgaag cgcttcgaga ccgtcccggg
    11281 gaggatcgtc gccccctgcc cggtctatct cgcccccgac ttcggcgcgg cgcgctgggg
    11341 ggagctcgag gacggcgacc cggtcgaggt cgtcctcgcc gaccacgact tcctcggagt
    11401 ccgcctcctc ggcatcgacc tcgccttcgt ccccgcgcac gccgtccgct acgtcgctcc
    11461 ggacgttccg acgccgcctg tgcccgcccc cgcggccccc gaggccgccg cgccgggacc
    11521 cggcggaccg gtcgacgtcc ccgccggggg ctttggcgcg gagacggccg cagacgcctc
    11581 ggccgagccg tacaccgcgc tcccgttcgg ggccacgccg cccgtcctga gaacgcgcgt
    11641 cgagccggcc tatcccgccg cggcccggaa agcggggctc ggcggggacg tcgtcctcca
    11701 ggtcgtcgtc gagcgcgacg gcacgatcgg gagcgtcttc gcggtccagg agggaccgct
    11761 cggcctgacg accgccgcga cggacgccgt gcgtcgctgg gtctacgagc ccgcgcgcct
    11821 cgacggccgg ccgatcgccg tcatgaagac ggtgcgcgtt cggttccagg ccgccgcccc
    11881 ctgaacggcg ggccgacggc gcagcctcct ataatcggct caccgccccg atgtacgagc
    11941 tcgtctacga ggtcgaaggc cgaccgcagc gtttccgcct gaccgggacc gaggtcttcc
    12001 tcggccggtc gagcgagaac gagatcgtcc tgaacgactt ctccgtctcc cggcggcacg
    12061 ccgtcatccg tcaggaggat ggcacctggg tcgtcatcga ccagaactcc acgaacggcg
    12121 tcaaggtcaa cgggaagttc gtcgcctcct ccccgatcgt tcccggcgac gtgctgaccg
    12181 tcgggacgtt ctccctcacc gtccgggagg aggcccccgc ggtccggccc ctcccgcccg
    12241 tcccggtcgt gggggagtcg tcctccacgt tcatccggtc gatcgccgac ttcaaccgcg
    12301 acttccacct cgaggactac gttcccgagg aggagggcga cgccctctct gcctccggga
    12361 agcggtcgac ctcggccgtc gcccggagca agatcttcga gatcctcgtc caggtcgcca
    12421 agacgctcat cctcgcccgc gaggtccacg aggtcctcgg ccgcgtcgtc gacctcctct
    12481 tcgagcacct gccggtcgac cgcgccgtcg tcgtcctcgt cggggacgag ggggggctcg
    12541 tcccgatgct cgcccgccag cgggggaagg agccgggaga ggccgaggag atcttctccc
    12601 acacgatcgt cgagtcggtc gtgaagaacc gcgtcgccgt cctcacctcc gacgccctcg
    12661 ccgacggccg cttcgaggcg gggcagtcga tccggatcca gcagatccgc tcggcgatgt
    12721 gcgtccctct ctggaaccag gaccgggtca tcggcgcgct ccacgtcgac acgccgctcc
    12781 gggtcgggag cttcacggcc gacgacctcg acctcctgac cgccctcggc aacttcgccg
    12841 ccgtcgccat cgagcgggcg cgcctccagc gccggatcga gcgggaggag cggatccgcg
    12901 agcgcctctc gcgctaccac tccccgggcg tcgtcgagga gatcgcctcc gacgcgagcg
    12961 gcgtcatcga gagggtgagg acccggaacg tcaccgtctt cttcgccgac atcgtcggct
    13021 tcacgagcgc ggccgagggg atggagcccg aggcgctctc gcgcttcctg tcgacgttct
    13081 tcacctacgc ggcggacgcg atcttcgccc agggggggac cctcgacaag ttcatcggcg
    13141 acgcggtcat ggcgttcttc ggcgcgccga tcgtccagcc cgaccacgcg cgccgggcgg
    13201 tcgccgcggc cgcgaagctc gtcgagaacg tcaaggcctg gaacctcgag cgggtcgcgc
    13261 gcggcgagcc gcccgtcggc gtgaggatcg gcgtcaacac cggcaacgtc gtcgtcggag
    13321 acatcggctc ggcgcagcgc gtcgactaca ccgtcctcgg caacaccgtg aacgtcgcgg
    13381 cccggatcga ggagtacgtc gccgggccga acgaggtcgc cgtcggcgag gagacggccc
    13441 gcctcacgtc ggacgacttc tcctacgagc cgctcggcga cgtgaagctc aagggcctct
    13501 cgcgcggcct gccgtgctac cgggtcaagc tcgacgcccg cggcgtccct ctgccggcgc
    13561 cgcgctgaat gcccccgagc ctcgaaaacc tcgtcttcct cacctcgaac gccggcaagg
    13621 cgcgcgaggc ctccgtcctc ctcggccgcc ccgtcgaggc gcgggcgctc gacctgcccg
    13681 agatccagtc cctcgacttc gtggaggtcg tcaccgcgaa ggcgctcgcc gcggccttgc
    13741 agctcggcgt gccggtcctc gtcgaggact ccggcctcgc gctccccggc tggaagggct
    13801 acccaggccc gctgacgaag tacgcggtcg gcgcagtcgg cgaggcgggc ttcgcgcgcc
    13861 tcgttcacgc ctggggcgac ccgcgcgccg tggccgtctc gacgctcggc ctcgcctggc
    13921 ccggcgcggc tcccgccgag gtcgtcgtcg ccgtggggcg cgtcccgggc tcgctcgcgg
    13981 ccgagccgcg cggcgcgagc ggcttcggct gggacgtcat cttcgtcccc gacggcgaga
    14041 cgcggacctt cgcccagatg agcgccgaag agaagaacgc ccgctcgcac cgggcgaagg
    14101 cgttcgcggc gctcgtggag cggctcggga tatcctcccg aggcggcgga ggggtgactt
    14161 cgtgatcgag cttctcggca atccctggtt cgcagcgacc tttctcgtct tcgcggccgc
    14221 cgttctcgtc ttcatcgcgc gtcgcaacag gacacgcgag gaggcggcga aggggccgtc
    14281 ccgttcgtct cagcgagccc gcacggaacc ggccgcgcgt tcggccgcgt ttgccgcggt
    14341 cccggcgccg gagagccgaa gccccggagc ctccctcgac gccgcgagcc ttccgcccgg
    14401 cttccgggac ctgacctggg ggagcgcccc ggtcgacggc atgcagctcg tcgccgagaa
    14461 cggcgaccat cgtttcctgt cccgtcccac cgactcgctg cggatcggac gggccgtcct
    14521 cacatcgatc acctacgtct atgagcgagg ccggcttcag gcgaccgtca tcgagctccc
    14581 ggcctcgggc ttcgagctgc tccagcgcca gctccgggag gactggggag cgccgaggac
    14641 gctgaagccc gaccagaagt acgcgtgggc cgacatgagg gcgagcgacg aggcgaccca
    14701 ggcgatcctc gagaagccgc ccgcgagccg gaccgctaag ctcgtcgtct ccgcgaaggc
    14761 cgtgagcacc gcccggaccc gggacgcggg cgcggccgac gccggagcgt aggttttctc
    14821 cggtacggcc ggcgaggggc cgttggtccg cccgcttccg atatcgagtc aggcatccac
    14881 tgcggaggac ccgtacctgc gtggggccat caccggcacc gaccagggtc gtccggacag
    14941 cccggaggtg taatgggggc tctcctcttc atcgtctcgt ggggcatcgt gatcgtcgga
    15001 gggcttccga agcgcacgca ggaggcgctc gagtttcgct tcggatggtg gtccgcgaag
    15061 gtggcgggcc tcctcagcgc cgctctcgaa ttgcatgcgg gataccgcct cgtgcgcgtc
    15121 acgctggccg cgttctccat ggagcgcgtg tcggtgacgg cggccgccat cttgtacgcc
    15181 atttccgcgt tcctccttct cgagggactc atacggttcc tggtgacgta ccggatgggc
    15241 gacctggccc ttccgtcgct tcccgtgtgg gcggtcgcgc ggctcatcga gtggctgcgc
    15301 ccgagtgacc gaagctcctc cgcgacggcg tgaggtcctc ttcgagggct gcgctcgaag
    15361 cgaaagggaa cgccgtcgag agtctccgac gcttcctccg tggctctcgt agaacctgaa
    15421 gaggcgcgag atcgaggagg acacgacggg gctcttcacg ttgccgctga cgaatctccg
    15481 cgcggggagc gaggagctcg acttcttcgt cccgacggcg tccgggaagt acgagggccc
    15541 gacgatgaag ctgttcctcc agctcgtccc gaaggccgag ctgaaggact acgcgcaggg
    15601 ccggaagctc cggcgcgtag agcgaagccc ggacttctgt tccgggccgt agcccgagtc
    15661 gctcccggcg tcaggcccgg gtgcccgacg cgcgggagcg gagccgcgcc gcgactgcct
    15721 cggccttcgt gctcagctcc tcgagcgtcc cgtcggtgac gatcacctcg tcgcaggcgg
    15781 cgttgcgctc ggcgtcgggg agctgggcgc gcatccgggc gaggacggtg tcgcgggtgg
    15841 cgcccgggtc ccgcgcgagg gcgcgggcga ggcggagctc ctcgggggcc gagacggcga
    15901 cgaggacgtc gtaccggtcg cgtccgttgt tctcgacgag gagcgtcgcc tcgacgaccg
    15961 cgaccggatg gccctcggac tcgcgctccg cgagccagcg ttcggtctcg acgcggacga
    16021 tcgggtggat ccgggcgttg agccgcgcga ccgtggccgg ttcggcgaag gcgaggcggg
    16081 cgaggcgggg acggtcgacg gcgccccgtt cgtcgaggag ctccgccccg aactcctccg
    16141 ccaccgcggc cgcgcccggc gcgcccggct cgtagagggc gtgaacgagc cggtcggcgt
    16201 cgaggacggg gatgccgagg gcgcgccagc gcgacccgac ggcgctcttg ccggaggcga
    16261 tgccgcccgt gaggccgacg cggagcatgc tcagagccgg cgccgggcgc ggcagagatc
    16321 gagggtgttc gcgaggaggc gggcgatcgt gagcggcccg acgccgccgg ggacgggcgt
    16381 gtagcgcgag gcgcgctcgg tcacgtcgac cgggtggacg tcgccgacga ggagcgagcc
    16441 cttcgccgcg aacgcggccg ccttcgcctc gttccccggg aagcagcggg cgacctcctc
    16501 ggcggtgtcg atccggttga tcccgacgtc gacgacgacg gcgccttccc ggatgaagtc
    16561 cgcggtgacg aaggcgggcc ggccgatcgc ggcgacgagg aggtcggcct cgcggcagag
    16621 ggcggggagg tcgcgcgtct tcgagtgggc gaccgtgacg gtggcgtcgg ccttcgtcag
    16681 gagcgtcgcc atcggcttgc cgacgatgtc gctccggccg aggacgacgg ctcgggtccc
    16741 gcggagcggg acgttctcgc ggcggaggag ctccatgacg ccggcggggg tgcaggggac
    16801 gggggcgggc ttgccctgga cgaggcggcc gacgttgaac gggtggaagc cgtcgacgtc
    16861 cttcgccggg tcgaccgcct cgaggatgcg ggaggagccg atcccggccg gaagggggag
    16921 ctggacgagg atcgcgtcga cctcggggtc ggcgttcagg cggccgatct cggcgaggag
    16981 catcgcttcg gtgacggtgg cggggaagcg aagggtctcg gcccggatgc cggcctcgcg
    17041 ggcccccttc tccttgttcc tcacgtagac ctggctcgcc gggtcgtccc ccgcgaggat
    17101 caccgcgagg ccggggacgg cgccgcgggc ggcgagctcg gaggcggcct cggccgtctc
    17161 gcggcggatg tcggcggcga cgcgcgcgcc gtccaggatc gtggctgtca tgacgctcct
    17221 tttcgcgcga cgtgcgcggc ggcgatgccg aaggagagga ggcggacctc gacggaagag
    17281 aagccggcct cgcgcatccg cccggagagg gcgtcggggg cgggccactt tccgacggag
    17341 tcggggaggt actggtaggc cgagctgtcc ttcgagacga ggcggccgag gaacgggacg
    17401 aaccggcgga cgtggaattt cacgaggggg gcgagggggc ccgtcggctc cgggacgaac
    17461 tcgaggacga cgagccggcc cccgggcacg aggacccggt gcagctcgcg caggccgcgg
    17521 tcgaggtcct cgaagttccg gacgccgaag gccgctgtcg cgccgtcgaa cgtccggtcg
    17581 gggaagggga ggctgaggcc gtcggccccg gcctcgggga tccgcgcgcc gcgccggagg
    17641 aacttcgccc ggccgaggac gagcatctcg aacgtgaagt cggccgccac gacgcggacg
    17701 ccggggctcc ggcgggcgac ggtgagggcg aggtcgcccg tgccggagca gaggtcgagg
    17761 atcctctccc cgggccgggg cgccaggagg cggacggctt cgttccgcca gacgacgtcg
    17821 gtgccgcccg agaggagccg attcaggagg tcgtagcggg gggcgatccg cgagaacatc
    17881 gcctggatcg tcccggaggc cttgtcgagt ccgggaggcg gggccgcgct cacggggcgt
    17941 cacggtagcc tccggaagcg ggtccgtaaa gcggcctttc ccggcgtccg gtgctttcgg
    18001 gtcgcatttc tccgagtctc gcttgacagg ccggaggccg cgtcgtatgt ttcccggccc
    18061 tgcgcgaggg cgaccgcgcg caaggtgcgt cctcccccgg gaaccggtct ttctcagtgc
    18121 cccggccggg tcctgaatac cgagacgaga agcgtggcgc ggtgcgcgct tcgccgaacc
    18181 ccgaccggag agctgagctt ccgtgagttc ccgaacgcct ctccccaaga tgaacgagct
    18241 cccgcggcgc tggatcgtcg tcgatgccga cggcaaggtc ctgggccgcc tcgcaacggt
    18301 cgtcgcgatg cacctgacgg gcaagtcgaa gccgacctac acgccgttcc tggacaccgg
    18361 cgacttcgtc gtcgtggtca acgccgagaa ggtcgcgctc acgggcgtca aggagaccgc
    18421 caagctgtac cggtaccaca ccggctaccc gggcggcctg aagacgacga gcgccgacaa
    18481 gctccgcgcg acccgtcccg agaagatcgt cgaggaggcc gtccggggga tgcttccgaa
    18541 gacgaagctc gggaagaaga tgttcaagaa gctgaaggtc tacgccgggc cgagccaccc
    18601 gcacgtggcc caggtcccga agccggtttc ggcgtgagtc gcgaggagag atcgtgagca
    18661 acctgcagta ccacgcgatc ggccggcgga aggaagcggt cgcccgcgtc tacctccgcc
    18721 cggggagcgg cgcgttcgtc gtcaacgacc gcccgttcga gaagtacttc ccgaacgacg
    18781 tcctcaagat ggtcatccgc cagcccctcc agctgacgga gtcgacggag aagttcgaca
    18841 tcgtcgcccg cgtcgcgggc ggcgggagcg cgggccaggc gggcgcgatc cgccacggcc
    18901 tcgcgcgcgc cctctgcctc ttcaacctcg agctgcggcc gagcctgaag aaggcgggcc
    18961 tcctcgttcg cgactcgcgg atgaaggagc ggaagaagta cggccagcgc ggcgcccgcg
    19021 cccggttcca gttctcgaag aggtagccat gagccagatc acgatgaagg agctcctcga
    19081 ggcgggcgtc cacttcgggc accagaccaa gcgctggaac ccgaagatga agaagtacat
    19141 cttcgggaag cgcaacggca tctacatcat cgacctgcag aagacgctga agtgcttccg
    19201 cgaggcggcg gccttcgtca ccgaggccgc ctcccagggg aagaacatcc tcttcgtcgg
    19261 gacgaagcgc caggcccagg acgccatcta cgaggaggcg aaccgctgcg gcatgttctt
    19321 cgtgaacaac cgctggctgg gcggcacgct cacgaacttc gtgacgatcc gcaagtcgat
    19381 catgcgcctc aaggagatcg aggggatgct cgccgagaac tcggaggcga tcctcacgaa
    19441 gaaggagcgc attcgcctcg agcgcgagcg gacgaagctc gccaagaacc tctccggcat
    19501 caccgagatg gaggagctcc cggacctcct cttcgtgatc gacccgaaga aggaggcgat
    19561 cgcggtgcag gaggcgaaca agctcggcat cccgatcgtc ggaatcgtcg acacgaactg
    19621 cgaccccgac ccgatcacgc acgtcatccc cggcaacgac gacgcgatcc gggcgatcaa
    19681 gctcttcacc ggcaagatcg ccgacgccgt cctcgagggc ctgcacgccc aggaggagcg
    19741 gttcgtcggg aaggccgcgg ag
//
LOCUS       DAOTEZ010000020        18468 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2007_length_18468_cov_6.828708, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000020 DAOTEZ010000000
VERSION     DAOTEZ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 18468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18468
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2007_length_18468_cov_6.828708"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="P5164_01940"
     CDS             complement(<1..447)
                     /locus_tag="P5164_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY42682.1"
                     /translation="MRILSVLTYYRPHWTGLTVHAVRLAEGLARRGHDVTVLTTRHLP
                     SLPREEVVDGVRVVRLEPVAGFSRGMIVPGLPRALARLLLRTDVVQMHTPLPEALLVA
                     GMARAFGVPLVMTHHGDVVMPESLREKAIETAAFVVLSGAARLASAV"
     gene            504..1370
                     /locus_tag="P5164_01945"
     CDS             504..1370
                     /locus_tag="P5164_01945"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRY42683.1"
                     /translation="MPDAHPLSAAIDRDALETFLASELDFAFRRRLRWLLTALDLRPS
                     LRLLDLGCGPGVLLRALDASAPRVRAAGLDEGADRLLQARAAPTPFPLLCADAQRLPF
                     RDASFERVLAAEVLEHLPDDAAALAEIRRVLAPGGLLAVSVPHADFPFLWDPINRVWT
                     RLGGAPFRTGPLVGIWTGHERLYRPEELSERVTSAGLEVVRVEEATHHAIPFAHFLLY
                     GVGRPLVERGLLPAGLRRRLGRLPSAPRPGFDLLSPLRALLSAVDRRNDADAVRRKTT
                     FVNVLLLARRPL"
     gene            1407..6494
                     /locus_tag="P5164_01950"
     CDS             1407..6494
                     /locus_tag="P5164_01950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HRY42684.1"
                     /translation="MSSPSAPDPVRAALRRVPPRRAFLLVAATAALAIAAGLLLAPGG
                     GHRAPLAWAGAAVALGEIARRVGRPEAREPAGPAAEGPGASRLARAALILLVLAGVSF
                     RLAWVRWDDGQLLHPDEYGLTGTLTRLALPKSVAEWFDTARSPMSPYARYDREGRAFP
                     GAAPDAAMRWGQWPQLIVRGVAELLSADAPRGGPDFTRFDALRILGRRLSALASVATL
                     GLLFLLARRVAGRAVALLATALAALAPLALQQARFLTVDGFAALFATAALLPAVRVAA
                     PGERAERSPWGDAALFGLLAAMSVASRLNLAPIVLLLPAALLVRGRGAKLLPLLYRAA
                     GPALLGAVVFLATFRVTHPMSFRAPRGETTAWTTTLNPDWIRNVELARSESALENSAP
                     PSEQWTGRTRLLFPARNTFLWGLGLPGGPLALAGLVLVGVALRAGRRRPPDGLLLLAA
                     WTAGFFLFMAVRPVSSVRYFLPLYPLFALFGALALARLAPPGAGAGRRAAAAVLLLLG
                     LAWAWGFTGAWRAPNTRVAASRAIYAAFPAATNLLLDEGSGPRRVPLRTERSSVVTEA
                     ERVVGLPAGTRGELRGVTFAHASGRPGSPLRVVLRGTAGGPVLAEGTHLLPGQPGHPL
                     GEAASVFLGPVRLSPERSYEIALSAPEGPVALSSSVLLGETWDEVLPCAVDGLDGGLY
                     DVRLLPVRGPDHAEKREAILRALAEADGVVLASPRSLWVVPRLPSNHPFTLAWYRALF
                     DGSVGFAPLPPSPFQAPLALGPLQLSDAAGTAAWGRPPALPPGGGSALAAEEAFWVYD
                     RAPVWLFGRKGSLPRAELEARLPASLLDDVVARTPLQASAAPGGLALSPERIAREEAG
                     AGRDAPPDPGAPQNARPALAVATWLLLLGLLAAAALPLTFAAFGRHPAAAFAAARLVG
                     LLAVGWLAFVLAALRVVPFGRNGLGVALAVLAAAGAATAWRTRAALGEALRRERRTFL
                     ATEGVFQLLFALGLFLRWGNPDLWHPSLGGEKPMALAFLNTLLRTSELPAQHPWFAGG
                     TLNYYDFGYLLAAVPARLLGLVPGLAYNLAVPTFFALSGTAAFALGASLVRSAAARGE
                     ERPAGAPFFAGLLSAAALVLLGNLGQLRLLLHAFAELGGGAAGTAGPVAVATGAMRAF
                     AGAPLPIRGEEWYWNATRLVPAVAGSGNEITEFPFFTYLFGDLHAHAMALPIGLLLLA
                     FALSLADAAPASRLGRGALLAVGALALGALRATNPWDVPVLTLLAVGAGAFGGARRAE
                     PGRRLRAALGWGLGPLALSALLFRPFDASYVAPYTSVARNAGAATPLAAWLWLWGLPA
                     FLVASLAALELARRRAVREEGGDRRAPLAGAGLLLGALAIGLVSGIPSGPVVLPLAVA
                     AGAVALAPGGGAGPERRAVLLLSAGALLVTFCVDVGSVGGDRMNTVFKLSFQAWALLA
                     VASGPALAWVVAALRDGGRTRPGPWTVTAGLLLGAAALYPVTATPARFAERFPRVAPG
                     AACRPFENAPAPPAALGAGAAGRPRGLDGEAFLADSALCVEGRLVPLRYDGEGFAWLR
                     ANERGSPAILEAVLPEYRWGSRFSVHTGLPAVVGWSWHVRQHRAAVPGSVVEKRIAEV
                     ERLYRSRLAAPGEGTGEGRATVEEVRRLLARHRVRWIVDGLLERAVYGEEGLAKLGEL
                     ERLGLLERAYASPGLTLWRVLPDATAR"
     gene            complement(6454..8394)
                     /locus_tag="P5164_01955"
     CDS             complement(6454..8394)
                     /locus_tag="P5164_01955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY42685.1"
                     /translation="MNAPAAREEGRESASLPARIEARSAALLGQPSWAASGLVLVALA
                     VAGSAGNRYTAWGAFAGFAALFALMRGFGDPDDDARPKRDGPSRAALAALVLVTLAAG
                     WLRVSALAPLPPEPTSDHVEKVLDALRIREGARPVFCPTNGGREPIQMYLLALLALLP
                     GVGLDLHSLTLLTAIEGTLTVPLLFLAGRELARVRGPAAADAAGLAAAALGAVSGWHL
                     VLSRLGLRIVLLPAATLLLLVFLLRAVREGRRRDWLLSGLVLGLSLHTYQSARFLLLV
                     VAAAAPLAALGSRAARARPLRNLAACAVLAAAGAAPLARYAVEFPADFWARARTRVLG
                     DAPSGDPLAALHARLPSLARGVLPALGALHATGDRSWFQAAPGRPLLDPASGALLLAG
                     LGLAGADALRRRDRAALLVPAALLVLLLPSAAAASFPDENPSATRASGALAPALLLSG
                     AALGRLGEALGRRRRSAGVLLGGALVAASALGARARLLEWKAAYAGRALPHRALGAEA
                     AAFTAAGRSFGNVFVVGVPHGVDDRAVAMEAGRPGRSPGLHPGERPLAQALAAARAGA
                     DGRRPDFDRAEPLLFLVSPRDAEGLAELSVLFPSARLRSVPATGPDGGFVVVEVPAER
                     PGPEPEREAVSAPSRPGAPARG"
     gene            complement(8391..10070)
                     /locus_tag="P5164_01960"
     CDS             complement(8391..10070)
                     /locus_tag="P5164_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRY42686.1"
                     /translation="MSRLRPRRASACALLLLAATLPAAAAKPASPAPEIDALLARALP
                     ADGPGVAVIAVKDGKTVYRKARGMASLELGVPLSPDSVFRLGSITKQFTAVAVLMLAE
                     EGKLSLTDPITKYLPGYPTQGHTITVEHLLTHTSGIRSYTGIPGYMTGPKVRADLTTA
                     ELVEVFSKEPMDFAPGAEWRYNNSGYVLLGAILEKVSGKPYGEFLAERIFRPLGMRST
                     RVGDDGPVLAKRAAGYTRDGETVANARFLSMTQPHAAGALVSTVDDLALWDAALYTEK
                     LVKRASLEKAWTPAVTRDGKPTRYGYGFGVSTLRGARSIEHGGGIFGFSTYAVRLPDE
                     KVYVAVLANSDAPAADPGTLGKEIAALLIGKPFPARVAVPLDPKLFERWVGVYRFDPQ
                     TTRTITAEGGRLFSQRSGGPRLELKPLSESEFFFDGSLTRIAFAAPDGKAKEALFYPE
                     GADEAEKGVREGDVPAPPSETKVDPALYDLYAGEYELAPGFILTVRRDGNRLLTRATG
                     QEELEVFPASETEFFLKVVDARITFVKGSDGKVTGLVLRQGGREMPAKRTR"
     gene            complement(10162..11715)
                     /locus_tag="P5164_01965"
     CDS             complement(10162..11715)
                     /locus_tag="P5164_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016359813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005416 - amino acid:monoatomic cation
                     symporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid carrier protein"
                     /protein_id="HRY42687.1"
                     /translation="MQHLFEVVNDLFAFFVPVSDLLWDFPKNVPSWAAIPVLGEFSFA
                     MLLLLGTGLFFTLRTGGVQLRRFGESVRIVMDRKASETGISPFAAFMLSSAMRAGPGN
                     IMGVTGAVTVGGPGALFWMWVAGTFGMATGFAEATLAQLFKERKESEFVGGLPFYGMR
                     ILGNRRWVGVTLAVLFITYALLNVPSQTFHLFTALGSVASTIAGTTYGRTSGVFYAIG
                     GLLVVSVAALVLGGLKRVIRFTDLCVPFMAVLYCGVVLLLVALNWRLVPWFLGSVFAG
                     AFSPEAIFGGSFGVALQQGIKRGLMVNEAGQGTITMAAAAADNRHPVEQGLVQSMGVW
                     FDTVVICSMAGFVTVMAKLWAAPEAFDWGARKADKLLVFMTSATHLTPGTGFDPAVKV
                     ALSVSYALFAFTTLIGMIVFAEISANMISRAHRFVFGIRAVGALFFVPFGVLTVLADL
                     QLGNIWYVTDLVNIVVASANVPVILAGARHVVAALRHYERTGGREGFVSERDIGLATP
                     YWTEEARKA"
     gene            complement(11726..13228)
                     /locus_tag="P5164_01970"
     CDS             complement(11726..13228)
                     /locus_tag="P5164_01970"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRY42688.1"
                     /translation="MQEPPRSDPAVRPAGGTPPTRRLSLRREALVTGLLALAWVAAWS
                     LGRIQEYAPHASLWFPPAGLTFAALAVLGPRAVPGVTAAAILVTLRLGDVYGVGYDLG
                     ALFVAGALFAAAHVGAFGLGAAALRRWSGSSVPQAVTAFLVVAPVSSALSAAGGAFAL
                     GTAGVIAPAEARAILLPWFVGDFVGVVALGPALAALLERLAATFRLRSTPLFGAVSRL
                     RPARPGWRRFVLLLALCLLPLAGSLVLLGGFGLRELATAFLVFFAVVPLMWIVHTEGS
                     ARAFGAVAALSVAIAGAGALAGRGEHTTAYQFAMIVLAGSAYFGLAVPSLYLDNEQLR
                     KLATTDPLTGAANRLAFDDAAIRETERARRFGTPLSLVLLDLDRFKVVNDTWGHGVGD
                     AVLVEVVDRLRAELREIDVLARVGGEELAVLLPMTQVHAAAETARRLAAALRGRPVTR
                     GAIAVPVTASFGVASVDPAAGFEAARERADRALYDAKRLGRDRVETADGS"
     gene            complement(13273..15087)
                     /locus_tag="P5164_01975"
     CDS             complement(13273..15087)
                     /locus_tag="P5164_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005996260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRY42689.1"
                     /translation="MPPLVAAQRLSKAFGARNLFTGLSFGIEPGERIGLIGPNGAGKS
                     TLLTLLAGEASPDEGSVVFQGGVSVGLVPQVPSFADGATVRSVVLQGALAHVSHGEEV
                     AWEAELKTDEALAKLSLTSGGLGPDSLVATLSGGQRKRVALARELVRQPDLLLLDEPT
                     NHLDVESIVWLEELLSRAPFATVTVTHDRLFLTRVATRVLELDRRNPGGLLSVPGGFP
                     EWLEAKEALLAAQESREASLRNVLRRETEWLRRGPKARATKQKARIERAGELAGEIEA
                     LEERNVSRTAKLDFAGASRAPKKLLVAEGISKAYGGRTLFAGLDLRLGPGSRLGLIGP
                     NGCGKSTLLRVLLGSERPDAGTVTRADGLSVAVFEQGRETLDPEATVKETICPEGDQV
                     EYRGRFLHFRSWLDRFLFSQEQAEMKVARLSGGEQARLLMARLMLRPAQLLVLDEPTN
                     DLDLATLNVLEEALAEFPGALLLVTHDRAFLDRATETLLAFDAAGGVTPLAGLDQWER
                     WRKERRETATRPAPKPAPAAPPKPAKRRLGYLEQRELDGMEERILGAEERLAALRADC
                     ERPEVVSDGPRLVTLAREIEETQAEVERLYARWAELSA"
     gene            15204..16253
                     /locus_tag="P5164_01980"
     CDS             15204..16253
                     /locus_tag="P5164_01980"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HRY42690.1"
                     /translation="MIKALGYGNTAATAPLERMEIDRRDPGPKDVRIDILYCGICHSD
                     LHTARNEWHGTTYPCIPGHEIVGRVTAVGAEVKGFKVGDMAGVGCMVDSCRSCSECKD
                     DLEQFCAKGAVFTYNSPDAHLGGMTYGGYTNVVVVDEAFVLKVPAGLDPAGAAPLLCA
                     GITTYSPLRHWGAGPGKKVGVVGLGGLGHMGVKLAHAMGAHVVLFTTSAGKTADAKRL
                     GADEVVLSKDEAAMKAHAGSFHFILDTVAAPHSLDAYTTLLRRDGTLCLVGAPEYPHP
                     SPTVFNLIFGRKSIAGSLIGGIKETQEMLDFCAETGLTADIETIPVQKVNEAYERMLK
                     SDVKYRFVIDMSSLK"
     gene            16361..16732
                     /locus_tag="P5164_01985"
     CDS             16361..16732
                     /locus_tag="P5164_01985"
                     /inference="COORDINATES: protein motif:HMM:NF014958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate isomerase"
                     /protein_id="HRY42691.1"
                     /translation="MPHLVLEHTANVPDRPDLHRVLLDLHDALLAAAPFERKDLKSRA
                     IRHDVFAVGDGADDRPFVALSIAILDGRRDDVKGAISDAALEVLVRAFPRLVAEGRGA
                     ISVEVRDLHRASYRRVRVREA"
     gene            complement(16743..17582)
                     /locus_tag="P5164_01990"
     CDS             complement(16743..17582)
                     /locus_tag="P5164_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRY42692.1"
                     /translation="MPRIVVVGSIAIDEVVALRRPLAAGLHLDGKEHGRRVGGGAANT
                     AIPLAFAGHDVVVVSAAGTDADGGTILRRLAEAGVDTSQVARLPGPSTRSLVLVDPAG
                     ERTVVNLHRCRESGPPRRLRSLPADAIYVRSRELDLARILAERLETSLVVAHVPPAVA
                     GARPAHVLVASASDLSPEEGERPWDALRRAGGETVRHVVVTRGGAGAEAHATDGRLRV
                     PAREAASVDSTGAGDVFAAGLLHALVSGAPMEAALRTAVAWGTASVECEGVPPRERIR
                     ALL"
     gene            17709..18224
                     /locus_tag="P5164_01995"
     CDS             17709..18224
                     /locus_tag="P5164_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRY42693.1"
                     /translation="MSTDSKSIASSLLPEFDLEMAGTRKVLERVKDEDWTFRPHEKSF
                     SMGELAGHVATIPEWLSGTLNAPSFDMAPGGVPAEFPRPGSAREALALFLEGVKAART
                     ALEATADPAFAEPWSLLENGKAWFTMPRGAVVRGFVMNHLIHHRAQLTVYLRLRDVPV
                     PALYGPSADES"
     gene            18254..>18468
                     /locus_tag="P5164_02000"
     CDS             18254..>18468
                     /locus_tag="P5164_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRY42694.1"
                     /translation="MHFCTHAAEIHGLFPDAKFLHMVRDGRDVVVSDCFLLMREREYR
                     GLSARAVAEVERARALLQVPEQPVLLL"
BASE COUNT         2333 a         6828 c         6912 g         2395 t
ORIGIN      
        1 gaccgccgag gcgaggcggg cggcccccga gaggacgacg aacgcggcgg tctcgatcgc
       61 cttctcgcgg aggctctcgg gcatcacgac gtcgccgtga tgggtcatca cgagcgggac
      121 gccgaacgcc cgcgccatcc cggcgacgag gagcgcctcc gggagcggcg tgtgcatctg
      181 gacgacgtcg gtgcggagga ggagccgggc gagggcgcgc gggagcccgg ggacgatcat
      241 cccgcggctg aagccggcga ccggctcgag gcggacgacg cggacgccgt cgacgacctc
      301 ctcccggggg agcgacggca ggtgccgcgt cgtcaggacc gtcacgtcgt gcccgcgccg
      361 ggcgaggccc tcggcgaggc ggaccgcgtg gaccgtcagg cccgtccagt gaggacggta
      421 gtaggtgaga acggagagga tccggatcgc gcgcctcgag cctgcggagg gtagcaggcc
      481 gcggaaccgg tactctccgc gcgatgcccg acgcgcatcc cctctccgcc gcgatcgatc
      541 gcgacgcgct cgagacgttc ctcgcctccg agctcgactt cgcgttccgg cgccgcctcc
      601 gctggctcct cacggctctc gacctccgcc cctcgctccg gctcctcgac ctcggctgcg
      661 ggccgggcgt cctcctccgc gccctcgacg cgagcgcgcc gcgcgtgcgt gccgcgggcc
      721 tggacgaggg ggccgatcga ctcctccagg cgcgcgcggc cccgacgccc ttcccgctcc
      781 tctgcgccga cgcgcagcgg ctcccgttcc gcgatgcctc gttcgagagg gtcctcgctg
      841 cggaagtcct cgagcacctt cccgacgacg cggccgcgct cgccgagatc cggcgcgtcc
      901 tcgcgccggg agggctcctc gcggtgtcgg taccgcacgc cgacttcccg ttcctctggg
      961 acccgatcaa ccgcgtctgg acgcgcctcg ggggcgcgcc gttccgcacg gggccgctcg
     1021 tgggcatctg gacgggacac gagaggctct atcggccgga ggagctctcg gagcgtgtca
     1081 cgtcggcggg cctcgaggtc gtgcgcgtgg aggaggcgac gcaccacgcg atcccgttcg
     1141 cgcacttcct cctctacggc gtcggccggc ccctcgtgga gcgcggcctt ctcccggccg
     1201 gcctccggcg ccgcctcggc cgcctcccgt ccgcgccgcg ccccggattc gatctgctgt
     1261 cgccgctccg ggcgctcctt tcggccgtcg accgccgcaa cgacgcggac gcagtccgac
     1321 gaaagacgac cttcgtgaac gtcctcctcc tcgcccgtcg ccccctctga ggcggcgccg
     1381 cgcctccggt atcgtcggag gatcccttga gcagcccctc cgctcccgac cccgtccgcg
     1441 cggcgctccg gcgcgtcccg cctcgacgcg ccttcctgct cgtcgcggcg accgcggcgc
     1501 tcgcgatcgc cgcgggactt ctcctcgcgc cgggaggggg gcaccgggcg ccgctcgcct
     1561 gggccggggc cgccgtcgcc ctcggcgaga tcgcgcgccg cgtcggccgg cccgaagccc
     1621 gggagcccgc aggccccgcg gccgaagggc cgggcgcctc gcgcctcgcg cgggcggcgc
     1681 tgatcctcct cgtcctcgcc ggcgtctcgt ttcgcctcgc ctgggtccgc tgggacgacg
     1741 ggcagctcct ccaccccgac gagtacgggt tgaccgggac gctgacgagg ctcgcgctcc
     1801 cgaagagcgt cgcggagtgg ttcgacacgg cgcgctcccc gatgagcccc tacgcgcgct
     1861 acgaccgtga ggggcgagcc tttccgggag cggcgcccga cgccgcgatg cgctgggggc
     1921 agtggccgca gctgatcgtc cggggggtcg ccgagctgct gagcgccgat gcgccccggg
     1981 gcggcccgga cttcacacgc ttcgacgccc tccgcatcct cgggcggcgc ctctcggccc
     2041 tcgcgagcgt cgcgacgctc gggctcctct tcctcctcgc gcgacgcgtc gcggggcggg
     2101 ccgtcgccct cctcgcgacg gcgctcgcgg ccctggcgcc cctcgcgctc cagcaggccc
     2161 gcttcctgac ggtcgacggc ttcgcggccc tcttcgcgac cgcggcgctc ctgccggccg
     2221 tgcgcgtcgc cgcgccgggg gagcgcgcag agcgctcgcc ctggggagac gcggcgctct
     2281 tcgggctcct cgcggcgatg tcggtcgcct cgcgtctgaa cctcgcgccg atcgtcctcc
     2341 tccttccggc agcgctcctc gttcggggcc ggggcgcgaa gctcctgccg ctcctctacc
     2401 gcgcggccgg tccggccctc ctcggggccg tcgtcttcct cgcgacgttc cgcgtgaccc
     2461 acccgatgtc gttccgcgcg ccgcgcggcg agacgaccgc gtggacgacg accctgaatc
     2521 ccgactggat ccggaacgtc gagctcgcgc gctccgagtc ggcgctcgag aactccgccc
     2581 ctccctccga gcagtggacc gggcggacgc gcctgctctt cccggcgcgg aacacgttcc
     2641 tctggggtct cggcctcccg ggcggaccgc tcgccctcgc cggcctcgtc ctcgtcgggg
     2701 tggcgctgcg cgcggggcgc cggcggccgc cggacgggct tctcctcctc gcggcgtgga
     2761 cggcgggctt cttcctgttc atggccgtgc ggcccgtctc gtcggtccgc tacttcctgc
     2821 cgctctaccc gctcttcgcc ctcttcggcg cgctcgccct cgcgcgcctc gcccccccgg
     2881 gcgccggcgc ggggcggcgc gcggccgcgg ccgtcctcct ccttctcggc cttgcgtggg
     2941 cgtggggctt cacgggagcc tggcgggcgc cgaacacgcg ggtcgccgcg tcgcgggcga
     3001 tctacgcggc gttccccgcg gcgacgaacc tcctcctcga cgaggggtcc ggcccgagac
     3061 gcgtcccgct ccggacggag cggagctcgg tcgtcacgga ggcggagcgc gtcgtcggcc
     3121 ttccggccgg aacgcgcggc gagctgcggg gcgtcacgtt cgcgcacgcc tccggacggc
     3181 ccggaagccc gctccgcgtc gtccttcggg ggacggcggg aggacccgtg ctcgccgaag
     3241 ggacgcacct cctccccggt cagccggggc acccgctcgg cgaggccgcc tcggtgtttc
     3301 tcggcccggt ccggctctcg ccggagcgga gctacgagat cgcgctctcg gctcccgaag
     3361 ggccggtcgc gctgtcgtcc tccgtcctcc tcggcgagac gtgggacgag gtcctcccgt
     3421 gtgcggtcga cggcctcgac ggaggcctct acgacgtccg cctccttccc gtccgcggcc
     3481 ccgaccacgc ggagaaacgg gaggcgatcc tccgggctct cgccgaggcc gacggcgtcg
     3541 tcctcgcgag cccacgctcc ctctgggtcg tccctcgcct cccgtcgaac caccccttca
     3601 cgctcgcctg gtaccgcgcc ctcttcgacg ggagcgtcgg cttcgcgccg ctgccgccga
     3661 gcccgttcca ggcgccgctc gccctgggcc cgcttcagct ctcggacgcg gcgggaacgg
     3721 ccgcctgggg acggcccccg gcgcttccgc cgggaggcgg ctccgcgctc gccgccgagg
     3781 aggcgttctg ggtgtacgac cgggcccccg tctggctctt cggccggaag gggtccctcc
     3841 cgcgcgcgga gctcgaggcc cgcctccccg cgtcgctcct cgacgacgtg gtcgcacgga
     3901 ctccgctgca ggcctcggcg gcgcccgggg ggctcgccct ctccccggag cggatcgcgc
     3961 gcgaggaggc gggcgccggg cgcgatgcgc cccccgaccc cggagcgccc cagaatgcga
     4021 ggcccgccct ggccgtcgcc acgtggctcc tcctcctcgg cctcctcgcg gcggccgcgc
     4081 tgccgctcac gttcgcggcg ttcggccggc atccggcggc ggcgttcgcc gcggcgcgcc
     4141 tcgtcggcct gctcgccgtc gggtggctcg cgttcgtcct cgccgcgctt cgggtcgtcc
     4201 cgttcgggcg gaacgggctc ggcgtggcgc tcgcggtcct cgccgccgcg ggcgctgcga
     4261 cggcgtggcg aacgcgcgcc gcgctcggcg aggcgctgcg gcgcgagcgg cggaccttcc
     4321 tcgcgaccga gggggtcttc cagctcctct tcgcgctcgg gctcttcctg cggtggggca
     4381 accccgacct ctggcatccg tccctcggcg gcgagaagcc gatggcgctc gccttcctga
     4441 acacgctcct gcggacttcg gagctccccg cgcagcaccc gtggttcgcg ggagggacgc
     4501 tcaactacta cgacttcggc taccttctcg cggccgtccc ggcgcgcctc ctcggcctcg
     4561 tcccggggct cgcctacaac ctcgccgtcc cgacgttctt cgccctctcc gggaccgccg
     4621 cgttcgccct cggcgcctcg ctcgtgagga gcgctgcggc gcgtggagag gagaggccgg
     4681 ccggcgcgcc tttcttcgcc ggcctcctct ccgccgcggc cctcgtcctc ctcgggaacc
     4741 tcgggcagct gcgcctcctc cttcacgcgt tcgccgagct cggcggaggc gcggccggaa
     4801 ccgccgggcc cgtcgcggtg gcgaccggag cgatgcgcgc gttcgcgggc gccccgctcc
     4861 cgatccgcgg ggaggagtgg tactggaacg cgacgcgcct cgtcccggcc gtggccggct
     4921 ccggcaacga gatcaccgag ttcccgttct tcacgtacct cttcggggac ctccacgcgc
     4981 acgccatggc gctgccgatc ggcctcctgc tcctggcgtt cgcgctctcg ctcgcggatg
     5041 cagccccggc gtcgcggctc ggccggggcg cgctcctcgc ggtcggcgcc ctcgcgctcg
     5101 gcgcgctccg tgccacgaac ccgtgggacg tccccgtcct gacgctcctg gccgtcggcg
     5161 cgggcgcgtt cggaggagcg cggcgcgcgg agccggggcg ccgcctgcgg gccgcgctcg
     5221 gctgggggct cggcccgctc gcgctctcgg ccctcctctt ccgcccgttc gacgcgtcct
     5281 acgtcgctcc ctacacgagc gtcgcgagga acgccggcgc cgcgacgccg ctcgcggcgt
     5341 ggctctggct ctgggggctc ccggcgtttc tcgtggcgag cctcgcggcg ctggagctcg
     5401 cccgaaggcg cgcggtacgc gaggagggag gggatcgacg ggcgcctctc gccggcgcag
     5461 ggctcctcct cggcgcgctg gcgatcggcc tcgtctcggg catcccgtcc gggccggtcg
     5521 tcctcccgct cgcggtggcc gcgggcgcag ttgcgctcgc cccggggggc ggcgccggcc
     5581 cggagaggcg cgccgttctc ctcctctcgg ccggggcgct cctcgtcacg ttctgcgtcg
     5641 acgtcggctc cgtcggcggc gaccggatga acaccgtctt caagctctcg ttccaggcgt
     5701 gggctctcct ggcggtggcg tcgggaccgg cgctcgcgtg ggtcgtcgcg gcgctccggg
     5761 acggggggcg cacgcgaccc ggcccctgga cggtgaccgc ggggctcctc ctcggcgcgg
     5821 cggcgctcta ccccgtcacg gcgacgccgg cgcgcttcgc ggagcgcttc ccgcgcgtcg
     5881 cgccgggtgc ggcctgccgg ccgttcgaga acgctccggc cccgccggcg gcgctcggcg
     5941 ccggcgcggc tggccgtccg cgcggcctcg acggtgaggc gttcctcgcc gactccgcgc
     6001 tctgcgtcga aggccggctc gtcccgctcc gatacgacgg ggaagggttc gcctggctcc
     6061 gcgccaacga gcgcggatcg cccgcgatcc tggaggccgt gctccccgag taccgctggg
     6121 gctcgcggtt cagcgtccac acagggctcc cggcggtcgt cggctggagc tggcacgtgc
     6181 gccagcaccg cgcggccgtt ccgggctccg tcgtcgagaa gcgaatcgcg gaggtcgaaa
     6241 gactctaccg gtcgcgcctc gccgcgcccg gggaggggac gggcgagggg agggcgaccg
     6301 tcgaggaggt gcgccggctc ctcgcgcgcc accgcgtccg ctggatcgtc gacggcctgc
     6361 tggagagggc ggtctacggc gaggaggggc tcgcgaagct cggcgagctc gagcgcctgg
     6421 gtctcctcga gcgggcgtac gcgagccccg gcctcaccct ctggcgggtg ctcccggacg
     6481 cgacggcgcg ctgacggctt cgcgttcggg ctccggcccg ggccgctccg ccgggacctc
     6541 caccacgacg aaccctccgt ccggcccggt cgcgggcacc gaccggagcc gcgccgaggg
     6601 gaagagcacg ctcagctcgg cgaggccctc cgcgtcgcgc ggagagacga ggaagaggag
     6661 cggctcggcc cgatcgaagt cgggccgccg tccgtccgct ccggcgcggg cggcggcgag
     6721 agcctgcgcg agcggccgct cgccggggtg aaggccggga ctccggccgg gccggcccgc
     6781 ctccatcgcg acggcccggt cgtcgacgcc gtgggggacg ccgacgacga agacgttccc
     6841 gaagctccgt cccgccgccg tgaaggcggc cgcctccgcg ccgagcgcgc gatgggggag
     6901 cgctcgtccg gcgtaggccg ccttccactc gagaaggcgc gcgcgcgctc cgagggcgga
     6961 ggcggcgacg agggctcctc cgaggaggac gcctgccgag cggcgccggc ggccgagggc
     7021 ttccccgagc cggccgagcg ccgcgccgga gaggaggagc gccggcgcga gcgcgccgct
     7081 cgcccgggtc gcgctcgggt tctcgtccgg gaaagacgcc gcggcggcgg acgggaggag
     7141 gaggacgagg agcgcggccg ggacgaggag cgcggcgcgg tcgcgacggc ggagggcgtc
     7201 ggccccggcg aggcccagcc cggcgaggag cagcgctccc gaggcgggat cgaggagcgg
     7261 gcgccctggc gcggcctgga accaggaacg gtctcccgtc gcgtggagcg ccccgagcgc
     7321 ggggaggacg ccccgggcaa ggctcggaag gcgtgcgtgc agcgccgcga gcggatcgcc
     7381 ggacggggcg tcgccgagga cgcgggtccg cgcccgggcc cagaagtcgg cggggaactc
     7441 gaccgcgtag cgggcgagcg gggccgcgcc tgccgcggcc aagacggcgc acgcggcgag
     7501 gttgcggagg gggcgcgcgc gggcggcgcg gcttccgagc gccgccagcg gagccgcagc
     7561 ggcgacgacg agaaggagga agcgagccga ctggtaggtg tggagcgaga gcccgaggac
     7621 gaggcccgac aggagccagt cgcggcggcg cccctcgcga acggcgcgga ggaggaagac
     7681 gaggaggagc agcgtcgccg ccgggaggag gacgatccgc aggccgagcc gggagaggac
     7741 gaggtgccag ccggagacgg cgccgagcgc cgcggccgcg aggcccgcgg cgtcggcggc
     7801 ggccgggccg cggacgcgcg ccagctcgcg cccggccagg aagaggagcg ggacggtgag
     7861 ggtcccctcg atcgccgtca ggagcgtcag ggagtggaga tcgaggccga cgccgggcag
     7921 gagcgcgagg agggcgagga gatacatctg gatcggctcc cgcccgccgt tcgtcggaca
     7981 gaagaccggc cgggcgccct cccggatgcg gagcgcgtcg aggaccttct cgacgtggtc
     8041 gctcgtcggc tcgggcggca gcggggcgag ggcggagact cggagccatc cggcggcgag
     8101 cgtgacgagg accagcgccg cgagagcggc gcgggagggg ccgtcccgct tcgggcgggc
     8161 gtcgtcgtcc gggtcgccga atccccgcat cagcgcgaag agcgcggcga acccggcgaa
     8221 ggcgccccag gccgtgtagc ggttcccggc gctcccggcg acggcgagcg cgacgaggac
     8281 gagccccgat gcggcccagc tcggctggcc gaggagcgcg gcggagcgcg cctcgatccg
     8341 cgcggggagg gacgcgctct cgcgtccctc ctcccgcgcg gcgggcgcgt tcaacgggtc
     8401 cgcttcgccg gcatctcgcg gcccccctgg cggaggacga ggccggtcac cttcccgtcg
     8461 ctccccttca cgaacgtgat ccgcgcgtcg acgaccttca ggaagaactc cgtctccgag
     8521 gcggggaaga cctcgagctc ctcctgcccc gtcgcgcgcg tcagcaggcg gttcccgtcc
     8581 cgccggacgg tcaggatgaa gccgggagcg agctcgtact ccccggcgta gaggtcgtag
     8641 agggccgggt cgaccttcgt ctccgacggg ggtgccggga cgtcgccctc gcggacgccc
     8701 ttctccgcct cgtcggcccc ctcggggtag aagagggcct ccttcgcctt cccgtcggga
     8761 gcggcgaacg cgatccgggt gagggagccg tcgaagaaga actcgctctc cgacaggggc
     8821 ttcagctcga ggcgcggccc gcccgagcgc tgggagaaga gcctcccccc ttcggcggtg
     8881 atcgtccggg tcgtctgcgg gtcgaagcgg tagacgccga cccagcgctc gaagagcttc
     8941 gggtcgaggg ggacggcgac ccgggccggg aagggcttcc cgatcaggag cgcggcgatc
     9001 tccttgccga gcgtccccgg gtcggccgcg ggggcgtcgc tgttcgcgag gacggcgacg
     9061 tagaccttct cgtccgggag cctcacggcg tacgtcgaga agccgaagat cccgccgccg
     9121 tgctcgatcg agcgggcgcc gcgaagcgtc gagacgccga agccgtagcc gtagcgcgtc
     9181 ggcttgccgt cgcgggtgac ggccggggtc caggccttct cgagcgaggc ccgcttcacg
     9241 agcttctccg tgtagagggc ggcgtcccag agcgcgaggt cgtcgacggt ggagacgagc
     9301 gcgccggccg cgtggggctg cgtcatgctc aggaagcggg cgttcgcgac cgtctcgccg
     9361 tcgcgcgtgt agccggcggc ccttttcgcg aggaccgggc cgtcgtctcc gacccgcgtc
     9421 gacctcatgc cgagcggccg gaagatccgc tcggcgagga attccccgta cggcttgccc
     9481 gagaccttct cgaggatcgc gccgaggagg acgtagccgg agttgttgta gcgccactcc
     9541 gcccccgggg cgaagtccat cggctccttc gagaagacct cgacgagctc cgcggtcgtc
     9601 aggtcggccc ggaccttcgg cccggtcatg tagccgggga tgccggtgta gctccggatc
     9661 cccgacgtgt gcgtcagcag gtgctcgacg gtgatcgtgt gcccttgcgt cgggtagccg
     9721 gggaggtatt tcgtgatcgg gtcggtgagc gagagcttcc cctcctcggc gagcatcagg
     9781 accgcgaccg cggtgaactg cttcgtgatc gagccgagac ggaagacgga gtcgggggag
     9841 agcggcacgc cgagctcgag gctcgccatc ccgcgggcct tccggtagac ggtcttcccg
     9901 tccttcaccg cgatcacggc gacgcccggg ccgtcggcgg ggagcgcccg ggcgaggagg
     9961 gcgtcgatct ccggcgccgg cgaggccggc ttcgcggcgg cagcgggaag ggttgcagcg
    10021 aggaggagaa gggcgcacgc ggacgcgcga cgcggtcgga gacggctcat cgggacacct
    10081 ctcttcgttc gggatcgggc gaccatgccg actctaacgg aagcgccccg tccggggttc
    10141 cgggtctgta ccgggcgggc ctcaggcttt ccgtgcctcc tccgtccagt acggcgtcgc
    10201 gagtccgatg tccctctcgg agacgaagcc ctcccggccg cccgtccgct cgtagtggcg
    10261 gagcgcggcg acgacgtgcc tcgctccggc caggatgacg ggcacgttcg ccgaggcgac
    10321 gacgatgttc acgaggtcgg tcacgtacca gatgttcccg agctggaggt cggcgaggac
    10381 ggtcaggacg ccgaagggga cgaagaagag cgcgccgacg gcccggatgc cgaagacgaa
    10441 gcggtgcgcg cgcgagatca tgttcgcgct gatctcggcg aagacgatca tcccgatgag
    10501 cgtcgtgaag gcgaagaggg cgtagctgac ggagagggcg accttcacgg cggggtcgaa
    10561 gccggtcccc ggcgtcaggt gcgtcgcgga cgtcatgaag acgaggagct tgtcggcttt
    10621 ccgcgccccc cagtcgaagg cctccggggc ggcccagagc ttcgccatca cggtcacgaa
    10681 gccggccatc gagcagatga ccacggtgtc gaaccagacc cccatcgact gcacgagccc
    10741 ctgctcgacg gggtgacggt tgtcggccgc ggccgcggcc atcgtgatcg tcccctgacc
    10801 ggcctcgttc accatcaggc cgcgcttgat cccctgctgg agcgcgacgc cgaagctccc
    10861 gccgaagatc gcctcggggg agaacgcccc ggcgaagacc gagccgagga accacggcac
    10921 gagccgccag ttcagggcga cgaggaggag gacgacgccg cagtagagga ccgccatgaa
    10981 cgggacgcag aggtcggtga agcggatcac gcgcttgagc ccgccgagga cgagggcggc
    11041 gaccgagacg acgagaagcc ctccgatcgc gtagaagacg cccgacgtcc gtccgtacgt
    11101 cgtccccgcg atcgtgctcg cgacggagcc gagcgccgtg aagaggtgga acgtctggct
    11161 cgggacgttc aggagggcgt acgtgatgaa gaggacggcg agcgtcacgc cgacccagcg
    11221 gcggttcccc aggatccgca tcccgtagaa cggcagcccg cccacgaact cgctctcctt
    11281 gcgctccttg aagagctggg cgagcgtcgc ctcggcgaag ccggtcgcca tcccgaaggt
    11341 cccggcgacc cacatccaga agagcgcgcc cgggccgccg acggtcaccg cgccggtgac
    11401 ccccatgatg ttccccgggc cggcgcgcat cgccgagctg agcatgaagg cggcgaaggg
    11461 gctgatcccc gtctccgacg ccttccggtc catgacgatc cggacgctct cgccgaagcg
    11521 ccggagctgg acgccgcccg tccggagcgt gaagaagagc cccgtcccga ggaggaggag
    11581 catcgcgaag gagaactcgc cgaggaccgg gatcgccgcc catgaaggga cgttcttcgg
    11641 gaagtcccag aggaggtcgg agaccggcac gaagaaggcg aacaggtcgt tgacgacctc
    11701 gaaaaggtgc tgcatcccgc tcccttcagc ttccgtctgc ggtctcgacc cggtcccggc
    11761 ccaggcgctt cgcatcgtac agggcgcggt cggcccgctc ccgggcggct tcgaagcccg
    11821 ccgcggggtc gaccgaggcg actccgaagc tggccgtgac gggcacggcg atcgcgccgc
    11881 gcgtcacggg ccgccccctg agcgccgcgg cgagccggcg ggcggtctcg gcggccgcgt
    11941 ggacctgcgt catcggcagc aggacggcga gctcctcgcc tccgacccgg gcgaggacgt
    12001 cgatctcccg gagctcggcg cggagccggt cgacgacctc cacgaggacc gcgtcgccga
    12061 ccccgtgccc ccaggtgtcg ttcacgacct tgaagcggtc gaggtcgagg aggacgagcg
    12121 acagcggcgt cccgaaccgg cgcgcgcgct ccgtctcgcg gatcgccgca tcgtcgaacg
    12181 cgaggcggtt cgcggcgccc gtcagcgggt cggtcgtggc gagcttccgg agctgctcgt
    12241 tgtcgaggta gagggacggc acggcgagcc cgaagtaggc cgagcccgcg aggacgatca
    12301 tcgcgaactg gtacgcggtc gtgtgctcgc cccggccggc gagggcgccg gcgcccgcga
    12361 tcgcgacgct cagcgcggcg acggcgccga acgcgcgggc cgacccctcc gtgtggacga
    12421 tccacatgag cgggacgacc gcgaagaaga cgaggaacgc ggtggccagc tcgcgcaggc
    12481 cgaagcctcc gagcaggacg agcgaaccgg cgaggggaag gaggcagagg gcgagcagga
    12541 ggacgaaccg gcgccatccc ggacgtgccg ggcgaagccg ggagaccgcc ccgaagaggg
    12601 gcgtggagcg caggcggaac gtcgcggcga ggcgctcgag gagcgcggcg agggcggggc
    12661 cgagcgcgac gacgccgacg aagtccccga cgaaccaggg gaggaggatc gcgcgcgcct
    12721 ccgcgggcgc gatcactccc gccgtgccga gcgcgaaggc gccgcccgcg gccgagaggg
    12781 cggacgagac gggcgccacg acgaggaacg cggtgaccgc ctgcggcacg ctgcttccgg
    12841 accagcgccg gagcgccgcc gccccgaggc cgaaggcgcc gacgtgggcc gcggcgaaga
    12901 gcgcccccgc gacgaagagg gccccgaggt cgtagccgac gccgtagacg tcgccgagcc
    12961 ggagcgtgac gaggatcgcc gcggccgtca cgccggggac ggcgcgtggc ccgaggacgg
    13021 caagcgccgc gaacgtgagg ccggccgggg ggaaccagag gctcgcgtgc ggcgcgtact
    13081 cctggatccg cccgagcgac caggcggcga cccaggcgag cgcgagcagg cccgtgacga
    13141 gggcttcccg ccggagcgac agccgacgcg tcggcggcgt tccgcccgcc gggcggacgg
    13201 ccgggtcgga acggggcggc tcctgcaccg gccggggacg ctagcagatc ggcgcggctg
    13261 ccgggaggcc ggtcaggcgg acagctcggc ccagcgggcg tacagccgct cgacctcggc
    13321 ctgcgtctct tcgatctctc gcgccagcgt gacgaggcgc gggccgtcgg agacgacctc
    13381 cggccgctcg cagtcggccc ggagcgcggc gagccgttcc tcggccccga ggatcctctc
    13441 ctccatcccg tcgagctcgc gctgctcgag gtagccgagg cgcctcttcg ccggcttcgg
    13501 cggcgccgcc ggagccggct tcggagcggg cctcgtcgcc gtctcgcgcc gctccttccg
    13561 ccaccgctcc cactggtcga ggccggcgag gggcgtgacg cctcctgccg cgtcgaacgc
    13621 gaggagcgtc tcggtcgccc ggtcgaggaa ggcgcggtcg tgcgtgacga ggaggagcgc
    13681 gccggggaac tcggcgagcg cctcctcgag gacgttcagc gtcgcgaggt cgaggtcgtt
    13741 cgtcggctcg tcgaggacga ggagctgcgc cgggcgcagc atgagccgcg ccatcaggag
    13801 tcgcgcctgc tcgccccccg acagccgcgc gaccttcatc tccgcctgct cctgcgagaa
    13861 gaggaagcgg tcgagccagc tccggaagtg gaggaagcgg ccgcggtact cgacctggtc
    13921 cccctccggg cagatcgtct ctttgaccgt cgcttccggg tcgagcgtct cgcgcccctg
    13981 ctcgaagacg gcgaccgaaa ggccgtccgc gcgcgtcacc gtcccggcgt cggggcgctc
    14041 ggagccgagg aggacgcgga ggagggtcga cttgccgcag ccgttcggcc cgatgaggcc
    14101 gaggcggctg ccggggccga ggcgaaggtc gagccccgcg aagagcgtcc ggccgccgta
    14161 ggccttcgag atcccttcgg cgacgaggag cttcttcggg gcgcgagagg cccccgcgaa
    14221 gtcgagcttc gccgtccgcg agacgttccg ctcctcgagc gcctcgatct ccccggcgag
    14281 ctccccggcc ctctcgatcc gcgccttctg cttcgtcgca cgcgccttcg gcccgcgcct
    14341 gagccactcc gtctcgcggc ggaggacgtt ccggagcgag gcctcgcggc tctcctgcgc
    14401 ggcgaggaga gcctccttcg cctcgagcca ctccgggaag ccgcccggga cggagaggag
    14461 accgccgggg ttcctccggt cgagctcgag gacccgcgtg gccacgcgcg tgaggaagag
    14521 ccggtcgtgc gtgacggtga cggtggcgaa cggcgcgcgc gacagcagct cctcgagcca
    14581 gacgatgctc tcgacgtcga ggtggttcgt cggctcgtcg aggaggagga ggtcgggctg
    14641 ccggacgagc tcgcgcgcga gggcgacccg cttcctctgc ccgcccgaga gcgtcgcgac
    14701 gaggctgtcg gggccgaggc ctcccgacgt gagcgagagc ttcgcgagcg cctcgtcggt
    14761 cttcagctcc gcctcccagg cgacctcctc gccgtgggag acgtgggcga gcgccccctg
    14821 gaggacgacg gagcggacgg tcgcgccgtc ggcgaaggac gggacctggg ggacgaggcc
    14881 gaccgagacg cccccctgga agacgacgct cccctcgtcg ggagacgcct ccccggcgag
    14941 gagcgtgagg agcgtcgact tgccggcccc gttcgggccg atcaggccga tccgctcgcc
    15001 cggctcgatc ccgaacgaga ggccggtgaa gaggttgcga gcgccgaacg ccttcgacag
    15061 gcgctgggcg gcgacgagcg gcggcacgtc gcgacgttag catcaggccc ggggaaggga
    15121 atccgggctt tacgaaacgg tcggaggcgt cgtatacgct gccgccgcgc gctccccctt
    15181 cccgcgtgca aggagaccag aagatgatca aggccctcgg ctacggaaac accgccgcca
    15241 ccgcccccct cgagcggatg gagatcgacc gccgcgaccc cggcccgaag gacgtgcgga
    15301 tcgacatcct ctactgcggc atctgccact ccgacctgca caccgcccgg aacgagtggc
    15361 acggcacgac ctacccctgt atccccggac acgagatcgt cggccgcgtg acggccgtcg
    15421 gcgccgaggt gaaaggcttc aaggtcggcg acatggccgg cgtcggctgc atggtcgact
    15481 cctgccggag ctgctcggag tgcaaggacg acctcgagca gttctgcgcg aagggggccg
    15541 tcttcacgta caacagcccc gacgcccacc tcggagggat gacgtacggc ggctacacga
    15601 acgtcgtcgt cgtcgacgag gcgttcgtcc tgaaggtccc ggcgggtctc gacccggccg
    15661 gagccgcgcc gctcctctgc gccggcatca cgacctactc gccgctccgc cactggggcg
    15721 ccggccccgg gaagaaggtc ggcgtcgtcg gcctcggcgg cctcggccac atgggggtca
    15781 agctcgccca cgccatgggc gcgcacgtcg tcctcttcac gacctccgcg gggaagaccg
    15841 ccgacgcgaa gcgcctcggc gccgacgagg tcgtcctctc gaaggacgag gcagcgatga
    15901 aggcgcacgc ggggagcttc cacttcatcc tcgacaccgt cgcggcgccc cacagcctcg
    15961 acgcctacac gacgctcctc cgccgcgacg gcacgctctg cctcgtcggc gcgccggagt
    16021 acccccaccc gtcgccgacc gtcttcaacc tcatcttcgg gaggaagtcc atcgccggct
    16081 ccctcatcgg cgggatcaag gagacgcagg agatgctcga cttctgcgcc gagaccgggc
    16141 tcacggccga catcgagacg atcccggtcc agaaagtgaa cgaagcctac gagcggatgc
    16201 tgaagtcgga cgtgaagtac cgcttcgtca tcgacatgtc ctcgctgaag tagcggcgcg
    16261 gagcgcgggc gccgccggga gggcggcgcc cgcgaagctc cgcgctcgtt cgcgtctccg
    16321 cggccggcgt cccgagtccg ccccgtcgga gaatggggcc atgccgcacc tcgtcctcga
    16381 gcacaccgcg aacgtccccg accggccgga cctgcaccgc gtcctcctcg accttcacga
    16441 cgcgctcctc gccgccgccc cgttcgagag gaaggacctc aagagccgcg cgatccggca
    16501 cgacgtcttt gccgtcgggg acggcgccga cgatcggccc ttcgtcgccc tctcgatcgc
    16561 gatcctggac ggacgccgcg acgacgtgaa gggggcgatc tcggacgcgg cgctggaggt
    16621 gctcgtccgg gccttcccgc ggctcgtggc ggaggggcgg ggcgcgatca gcgtggaggt
    16681 ccgcgacctc caccgggcga gctaccggag ggtccgcgtc cgggaggcgt gacggagcgc
    16741 cttcagagaa gggcgcggat ccgctcgcgc ggcggcaccc cctcgcattc gacggacgcg
    16801 gtgccccatg cgacggccgt ccggagcgcc gcctccatcg gggcgcccga gacgagcgcg
    16861 tggaggaggc cggcggcgaa gacgtctccc gcgccggtgg agtcgacgga ggcggcctcg
    16921 cgagccggga cccggagccg gccgtcggtc gcgtgggcct cggctcccgc gccgccgcgc
    16981 gtgacgacga cgtgccggac cgtctcgccg cctgccctcc ggagggcgtc ccacggtctc
    17041 tccccctcct ccggcgaaag gtccgacgca gaggcgacga ggacgtgcgc cgggcgcgcg
    17101 ccggccacgg cgggcgggac gtgtgcgacg acgagcgacg tctcgagccg ctcggcgagg
    17161 atccgggcga ggtccagctc tcggctcctc acgtagatcg cgtcggccgg gagggagcga
    17221 agccgccgag gcggcccgga ctcgcggcag cggtggaggt tcacgaccgt ccgctccccg
    17281 gccgggtcca cgaggacgag cgaccgggtc gacggcccgg ggagccgcgc gacctgcgac
    17341 gtgtcgaccc ccgcctcggc gagccggcgg aggatcgtgc cgccgtccgc gtcggtcccg
    17401 gcggccgaga cgacgacgac gtcgtgcccg gcgaacgcga gcgggatcgc ggtgttcgcg
    17461 gcgccgccgc cgacgcgtcg tccatgttcc ttcccgtcga ggtggaggcc ggcggcgagc
    17521 ggccgccgca gggcgacgac ctcgtcgatg gcgatcgacc cgacgacgac gatccggggc
    17581 atgtcgacgg aaggctagac ccttccgtga tcgtgcgttc ccgcgatccg cgacgacggg
    17641 tcgaactttc ccttgctctt tggtcagact tacgctttac tcgcgccaca ggatcaagga
    17701 ggcctcccat gagcaccgat tcgaagagca tcgcctcgtc tctccttccc gagttcgacc
    17761 tcgagatggc cggcacccga aaggtcctcg agcgcgtgaa ggacgaagac tggacgttca
    17821 ggccccacga gaagtcgttc tcgatgggcg agctcgccgg ccacgtcgcg acgatcccgg
    17881 agtggctgtc cgggacgctc aacgccccct cgttcgacat ggctcccgga ggggttccgg
    17941 ccgagtttcc ccggcccggt tcggcgcgcg aggccctcgc gctcttcctg gaaggggtga
    18001 aggccgcgcg cacggccctc gaggcgacgg ccgaccccgc cttcgccgag ccgtggtccc
    18061 tcctcgagaa cgggaaggcc tggttcacga tgccccgggg cgccgtcgtg aggggcttcg
    18121 tgatgaacca cctgatccac caccgcgcgc agctgacggt ctatctccgg ctgcgcgacg
    18181 tcccggtccc cgcgctctac gggccttctg ccgacgagag ctgaagctga agtggatcgg
    18241 ggataaaacc acgatgcact tctgcacgca cgcggcggag attcacgggc tgtttccgga
    18301 tgcgaagttc ctgcacatgg tgcgcgacgg gcgggatgtg gtggtcagcg attgtttctt
    18361 gctgatgcgc gagcgcgagt atcgcgggtt gagcgcgagg gcggtggcgg aggtggagcg
    18421 ggcgcgggcg ctgcttcagg tgccggagca accggtgctt ctactgca
//
LOCUS       DAOTEZ010000021        18462 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2010_length_18462_cov_6.913457, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000021 DAOTEZ010000000
VERSION     DAOTEZ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 18462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18462
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2010_length_18462_cov_6.913457"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="P5164_02005"
     CDS             complement(<1..349)
                     /locus_tag="P5164_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42695.1"
                     /translation="MRGPRRLSQQLILSLTVVVSLVVVANGVIAVRSADTQLQATMLQ
                     SVEQLSGAIASSTWHAMLADNRESAYQVMQTIAAKQGIRSVRIFNKEGRVMFSTPRSE
                     VKVVDKKAEACFLC"
     gene            complement(480..2564)
                     /locus_tag="P5164_02010"
     CDS             complement(480..2564)
                     /locus_tag="P5164_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42696.1"
                     /translation="MRIVLAATVLAASFSLGGAVSAAVAPSVSPDALKSLEAELVERH
                     GEASRPRVARGLVQAAALWRASDGDAAAFAEVARTHFLSDPAKLDALFSRMERVLESV
                     GGHMLEVVRDLREHSDLDRGEILPFDDVMAAWDPSAHVADDLFSNRLAFAVLLNFPLT
                     TLPERLEKGPAWSRREWAEARLAEGFGKRVPAEVNQAVATASADASRYISEYNLRVHH
                     LLDEKGRRLFPQGKRLLSHWNLRDEIKALYGDGKDGLAKQRAIARAMERIATQTIPAA
                     AIDSPHVDWNPFTNEVKATTSLEPGAPPLPAGFAPAAAPEPDARYAKLLATFRASRLV
                     DPYSPAAPTLIDRRFAEDRQLPEARVKAMLESVLSSPAFAETGKLVAKRLGRPLQPFD
                     VWYPGFRERGPYTEAQLDEITRKKYPTPEAFEKDIPRILAALGFPEERARWIASNVVV
                     DPARGSGHAWGAGRRGDKAHLRTRVGRDGMDYKGYNIAVHELGHNVEQTISLHLVDHT
                     LLSGVPNTAFTEAIAFVFQERDLPLLGLPKGDAKGRALATLNTFWGTAEIAGVALVDM
                     AVWRWMYEHPEATPAQLKEAALSIAKETWNRFYAPVFGVKDSPILAVYSHMIDSFLYL
                     PDYPVGHLIAFQIERHVEKAGAVGPEVERMTRQGRISPDLWMQGAVGAPVGAEALVEA
                     AAEALAELGRGR"
     gene            complement(2617..4128)
                     /locus_tag="P5164_02015"
     CDS             complement(2617..4128)
                     /locus_tag="P5164_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42697.1"
                     /translation="MDSAAGSSYHPGGGDVRDDDRRDAPDLPELLEILGEVAERVGAL
                     SIARRSPASRQEIEELRERLLFARHLLEGLFALRAEPVPDPEPGVAFEALGPGLRRTL
                     PQRLAAQLEDERNAVTERSLRELSSFAAFGLHARVVELLRLVDWVTRLAAEEARRAAP
                     PTVISLRHAPRATAKGGARQAPREAAPAGPPPEAAPPAPRPAPPPAPREDPGPDLSSY
                     DLRIDEAGERVDVAGVSAWFASAAGPQAEGPGLAASFAVAFPGGIALAVATGAESSLG
                     ARLAAVVAARTFCRAAAASPSDPLAAVRTAQGHLEMLLAALLTAGDSSDALTRVRGSM
                     APGSARRVLAHTRAPDPALRRVPPALACGLVGAVATAAGGRVEATVVRLGPGTVQARR
                     GGAPSPVPFARQLPEPSFLAPGTLGAGGLTRAEAAPAVSLARGDALLLATPAVGRGAP
                     SAWTSLATLWPPFPDGLGDGASARRLLERAERWGVAEPPSFAGPLGIGVLLAR"
     gene            4165..8739
                     /locus_tag="P5164_02020"
     CDS             4165..8739
                     /locus_tag="P5164_02020"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY42698.1"
                     /translation="MSRGPSLPESLDRWERTPSPERAAELLESARTALSAGNAPPSLW
                     HRWLDATRRSEHLLALGDREARHRWADAAIGAIHASGYDLARLLAQRVAAHPERALLV
                     DGPAPGAPRWSYAHVARRADAIAAALLALEAEPRVAVVAENGVDSACLDLGCLLHGLL
                     VAPLSPHLTAGELVAAFDALGVNLAVVASEESLRRVREAARRTVRPLRVVLLDEAAEL
                     ASEGDVVLGEALAAQAEAAVPTLLARAPRRALHETCTVLFTSGSTGGQKGVAFSMANL
                     VTKRFARAAALPFVGEEETLLCFLPLYHTFGRYLELLGMLFWGGTYVFAGNPSLDGLA
                     AGLRTVEPTGLISIPIRWAQLESRAREAAGEAPSPETARAALRSVVGGRLRWGLSAAG
                     YLAPQVFRYFQAHGVELCSGFGMTEATGGITMTPPGEYEEGSVGLPLPGIRVRLAEHD
                     ELQIEGAYVARYLDEPETAPGGERWLPTGDVFRVRPSGHLEIVDRVKDIYKNSRGRTI
                     APRRVESRFEGVPGVRRVFLAGDGRDDNVLLVVPDADDPVLAGDPQGESARDYLRRIV
                     AAANADLAPYERVVDFAVLARDFSLEEGELTPKGSYRRKAIEKSFAPVIEGLYRSRVV
                     RLAGDGVEVRAPRWLLRDLGLLEGDLVVEGGRLVDRRHGRSAALAARGDGDEVRVGNL
                     SYRCAGRSVDLGLLARQPLLWLGNPELAALLPVKDGWDTPLTGSCAEVLLPPRVEVPD
                     GTDVPLPAPLPSPQLTALHKLSVALLFGPPESALEAVARAEALLRQGDRRTAELLRRR
                     LQALARHPDVRVRCEAYRVLLLDDPLVDSGEILPAFLRSGLPFLSEESVAAIARADLG
                     EERLTALRRRLHSYREAMTGPLPETTRTTLGDVLELLGDFARHHPEDHAAVRAELASW
                     AIQERDPELARRADALFDSLGVWHEETAAAAALLPPVSEGRKKLSFRDGMPEEEVRTL
                     EGLLLGTSFLRESIELAFETDPPELADIPEEGIWVTPVQALQRHRLYRLSVRTATGKH
                     HDLLLTVRGDLDSAAVRATTLRMIALAGHPWGPPVVPRVGSCRPDLGALSLAWVDELT
                     LAERLRDAAVPGRARPGEGHSVKSLVVRALAAFFTAWERSGRLLVPGLVTPSNVAVPA
                     EDYRDGARLLSISGARTYTGPLDLVAPMLRNAFREPAALSPRLAAVLSPAWIVDAVFE
                     ALGEEGARAFLAELREAAGAPSSEAWGRLLAAVDAALAAAPGERTPLALETAVARYEA
                     WEAERPGVSLAERERTAESLLRLYRVDTLGEEARYRLFRKTVFARSAPPVLGAFDALL
                     AALRARPGSRPTHLVELQGLHAALSTDAERHAFTRLVFPAGRSTRPVEVSVVGDAVKH
                     VVVATELVDRMDGAWLVREPVDAAEVGRLYRLFVRAGLPRAFSPDRRYLVVLDASERI
                     VGGVSWSQLSPDAAQLDGLVVAPSVRGRGLSSALLDDLSARLASLGVRSLLAHFAFRN
                     IPLPGFRLDRRHGGLVRDLSAGLEVEAGLA"
     gene            8750..9169
                     /locus_tag="P5164_02025"
     CDS             8750..9169
                     /locus_tag="P5164_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRY42699.1"
                     /translation="MKQLQERLELPTPGRGLHEVTEAVAAVVARSGVATGLCVVFCPH
                     TSARLLLQENADPTARADLLSWLSRLAPDGDPRYAHDAEGDDDMAAHLRSAVTRSSET
                     IPVSGGRLALGTWQGLFLAEHRTTPHRRRIVVHVFGE"
     gene            9171..11915
                     /locus_tag="P5164_02030"
     CDS             9171..11915
                     /locus_tag="P5164_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRY42700.1"
                     /translation="MRGACPARALRAVLAALALLALPAGAVSSVPDPPSAASSALAVA
                     PGFVVPASYRSPADGVERGYALRVPRTWDGTSLLPAILLLHGRGGTVRQFQRTPYFDA
                     ADARGVVLVFWEGRIAPGGAGVPSTHYVDGADGVPDETDVLACLDDALARAPIDADRV
                     ALAGFSQGGRGALGIGLLNPSRFAALVDAAGPTDAFQGQLWSPAFPDYFSAAGGPPSS
                     GGEVLARWHELSPRFLLPNARNLFVAVLHGQADDVVPDSAAYFPYRNSHHVTVTPGFS
                     DARGRTPTLAELHADDPAGYAFSTGFPDDVGHDQARLLTATALFEAALGKTRPARPAR
                     VVGVAFGARERTFHWARLGRRAPADGTRVLVDARSDFPANRVDLRVEGPARVRLELPG
                     AGLDASRALLVRVETAGAFDLALAGPFPPAVAATLDGLPIPLVRTAEGVVFPGLASAT
                     PGSLLVVAPSPAGPIAEGDLLVPALVRTEGANGARYDTVLALANLSDAPLSLEALLLD
                     GTSAPVPVLLPPESSRAVPAEALRTAPGSFAAPLRLRVVAGESAALASSARVFSRADT
                     GTYGLSFPVLPAGESVLAAGETGLLFGPRDPAAERLNVSLFAPFGASTAEVTVFDGRG
                     LALRPRPVALAALARVQLDDLLRGEAPGAHVRVAVLSGRLQVYGTAVSNGRTNDPWRV
                     PALRLAGAARSWTVPAVASAGGRNGAYFRSDLFLLAAEEASLDAMLLPRDGSPPETAR
                     LAVPGGAPHVVEDLLAVLFPSKAPGAGALLLSSTLPFLPLAVTRSEPPTGPSSQDLPC
                     VPEGGEVVPGRPVAFAGVDESSSARSNLVLVAPAAAARVRLRLFVPSGPLGERVVDLP
                     AGRVVQLDSVAARFTDDPVEGGTLLVSCESGAVVASVARIDAATNDPAGLAPLPVVVR
                     "
     gene            complement(11919..12689)
                     /locus_tag="P5164_02035"
     CDS             complement(11919..12689)
                     /locus_tag="P5164_02035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY42701.1"
                     /translation="MRAAVSVVFIVKDEADRLAPSLASVSWADEVLVADTGSTDGTQE
                     LAREAGARVETIPWEGYVATRNRAISLAANDWVLVLDADETVTAELAAEIREAVAGPD
                     GADGFRMSRLSHLGSRPIRHGVWWPDRKLRLGRRSRGFRAEGGHVHEWIEVDGKVRDL
                     SRPLLHHPYRDLADAVRKIVLYARLSALERFERGGRGNVLLLVLRPPLEFLRSYVLKR
                     GFLDGRAGVAVALLHAWYYLLRAGFLLEATERAKLERA"
     gene            12711..13724
                     /gene="waaF"
                     /locus_tag="P5164_02040"
     CDS             12711..13724
                     /gene="waaF"
                     /locus_tag="P5164_02040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HRY42702.1"
                     /translation="MRLLLRATNWVGDVVISLPALRALRAHHRGDHLAVLARPWVADL
                     YRLLPEVDELLVEEPRGRHAGREGRRLLADELREKAFDRAILLPRSFATARTVSRAGI
                     PERWGFRGELRSPLLTHPVPFSPRPGEHELHRHLRLVEATGVPGPEIPDATIAVPDAV
                     RRGARAKLAEAGLASGPFAAAHVASFAHEAKRWPEERFAALFARLHEERGLPVVLLGS
                     AAEAGMNARVAALAAPARVVDLAGRTSLAEVLGVVAEAELFVGNDSGLAHLSNAAGTP
                     TTVVFGPTDPDATRPWDGPRPDGKPVRLRIVRNRVPCAPCRFTRCPLDHACMTGLTFE
                     LPA"
     gene            complement(13824..14372)
                     /locus_tag="P5164_02045"
     CDS             complement(13824..14372)
                     /locus_tag="P5164_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRY42703.1"
                     /translation="MIDAHLSALAETALAARGAMKPAIEAAAAAVATAVRAGNKALAF
                     GNGGSATQAQHFAAELVVRYKGDRPGLPAVALVCDGAILTACANDYSYDYVFARQVEA
                     LGRPGDVALALSTSGTSPSVVKALEAARARGMVTILVTGTRGAAEAARWDHGLVVPTA
                     ETAHVQEVTLAVLHLLCAPLDA"
     gene            complement(14369..15832)
                     /gene="rfaE2"
                     /locus_tag="P5164_02050"
     CDS             complement(14369..15832)
                     /gene="rfaE2"
                     /locus_tag="P5164_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HRY42704.1"
                     /translation="MTPRELAALAAAAPSVKAVVVGDLMLDETWLGDVSRVAPEAPVP
                     LLALSSMCRRAGGAGNVVENLAALGARTVALGAVGPEEEGAWIARHLGSLPGGEGRVV
                     VDPLRTTPVKRRMVADGRQLLRVDQELPAVLSKGAEEELIGAALAALESADVLLLSDY
                     AKGTLTPRLMETLLAEARRRGVPALVDPKGKDYGRYRGATCVTPNRKELETVTGESAR
                     DAAAVVRLATRLRDDLGLDAILVKLSEEGMLLLPKGGAPHRIAARAREVFDVTGAGDT
                     VLATLGVALGAGLPLADAAEVANRAAGVVVGRTGTASVRWADLLDGLTSAPADKLLER
                     GHVGAVAQMLRRAHRRVVFTNGCFDLLHPGHVTLLGAAKQLGDVLVVGLNSDASVKRL
                     KGPERPILSETDRAQVLGGLDAVDFVVLFEEDTPEALIRAIRPQVLVKGGDYTEESVV
                     GAPFVRSYGGEVALVPLVPGRSTTSMVAKMSKTKEAP"
     gene            complement(15852..16805)
                     /locus_tag="P5164_02055"
     CDS             complement(15852..16805)
                     /locus_tag="P5164_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42705.1"
                     /translation="MTTTKRRRPRETVTTFPIATRSFKVKADDFAAKPSTSGAFADFW
                     NGLPRILAAESLRKVVSAIHAARRKGKPVVVAFGAHVLKTGLGPILIDLVERGFVTAL
                     ATNGASGIHDFEVAFHGKTSEDVGPVLETGEFGMSRETGVGVFRALRAGRRNGVGYGR
                     ALGEGILRARLPFRHLSLFAAAAKADVPLTVHVALGTDTLVQHPGMDGALVGEMSIRD
                     FTTIRRVIAGLGDGGVWINAGSAVIMPEVFLKALSMARNLGHDVRNFTTVDLDMNRHY
                     RPHENVLKRPVLSGGASYAITGHHEIVLPLLAQALVLGLEE"
     gene            complement(16802..17398)
                     /locus_tag="P5164_02060"
     CDS             complement(16802..17398)
                     /locus_tag="P5164_02060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HRY42706.1"
                     /translation="MKRRPLLDALFLDRDGTLIAERGFLADPKGVRLAPGAAPLLARF
                     ASEGTSLFVVTNQSGVARGLTRWDEVSAVNAEVERRLAARGVPIAGWLVCPHHPEGRV
                     KEYAVACRCRKPGTLLHRRALRRHGLAAERSAVVGDKWDDVGAGLALGAIAVHLLSGH
                     GREHRARVREKGGGRVILAGGLADGLRRLRAAAEGKNG"
     gene            complement(17395..17922)
                     /locus_tag="P5164_02065"
     CDS             complement(17395..17922)
                     /locus_tag="P5164_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRY42707.1"
                     /translation="MAGTESPTGTRKTAAAVLAAVFLAGAASASAEPVKHLLPRDYSA
                     RVVAGKKGRVLVVNFWATWCEPCREEMPYLVAAAKKFPSRDLAVTLVSLDSQKTGPSQ
                     VPKFLKEQKVPFVCWLAKSRDPQDFIDTVDRTWDGSLPYTLVYGRDGKIAARLSGLQT
                     EASFTEAIRKAVGRK"
     gene            complement(17925..>18462)
                     /locus_tag="P5164_02070"
     CDS             complement(17925..>18462)
                     /locus_tag="P5164_02070"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRY42708.1"
                     /translation="PAEPPAAPRIGSAAPDFTLPPAAGGAAVALKDLLGKSKAVVVIF
                     DATKCPYAKGYKERVAAMGKEYAAKGITFVTINSNKTEPAAEVAEDAKKNGFAFPVLK
                     DEGNRVADLYGAKKTPEVYVLSPAGTLLYHGRIDETHEDPKNVTSPDLRNALDAILAG
                     KPVPAAETKAFGCTIKRV"
BASE COUNT         2169 a         7131 c         6659 g         2503 t
ORIGIN      
        1 gacagaggaa gcaggcctcc gccttcttgt cgacgacctt cacctcggac cgcggggtcg
       61 agaacatgac ccgcccttcc ttgttgaaga tcctcacgct ccggatcccc tgcttggcgg
      121 cgatcgtctg catgacctgg taggccgact cccggttgtc ggcgagcatg gcgtgccagg
      181 tggagctcgc gatcgccccg gagagctgct cgacgctctg gagcatcgtc gcctggagct
      241 gggtgtcggc cgatcggacg gcgatgacgc cgttcgccac gacgacgagc gagacgacga
      301 ccgtcagcga gaggatgagc tgctgggaga gccgcctcgg tccgcgcatc gtccgtccgc
      361 cccggccgtc acgcgccccg ggatcgggat cggacggggc cacaggtcat cgtacaccgc
      421 ggggacgccg gaaccgctcc ggcgtccccg cgcgggcctc gcccggcctc ccggccgggt
      481 cagcggcccc ggccgagctc ggcgagcgcc tccgccgccg cttcgacgag cgcctcggcg
      541 ccgacgggcg ccccgaccgc cccctgcatc cagaggtccg gcgagatccg cccctgccgc
      601 gtcatccgct ccacctccgg gccgacggcg ccggccttct ccacgtgccg ctcgatctgg
      661 aacgcgatca ggtggccgac gggatagtcg gggaggtaga ggaaggagtc gatcatgtgg
      721 gaatagacgg cgaggatcgg cgagtccttc acgccgaaga ccggagcgta aaagcggttc
      781 caggtctcct tcgcgatcga gagggccgct tccttcagct gggccggcgt cgcctcgggg
      841 tgctcgtaca tccaccgcca gacggccatg tcgacgaggg cgaccccggc gatctccgcc
      901 gttccccaga acgtgttgag cgtcgcgagg gcccggccct tcgcgtcccc cttcgggagg
      961 ccgaggagcg gcaggtcgcg ctcctggaag acgaaggcga tcgcctcggt gaacgccgtg
     1021 ttcgggacgc cggagaggag cgtgtggtcg acgaggtgga gggagatcgt ctgctcgacg
     1081 ttgtggccga gctcgtggac ggcgatgttg tagcccttgt agtccatccc gtcccggccc
     1141 acgcgggtcc gcaggtgggc cttgtcgccc cggcggcccg cgccccaggc gtggccggag
     1201 ccgcgcgccg gatcgacgac gacgttcgac gcgatccacc gggcccgctc ctcggggaag
     1261 ccgagggcgg cgaggatccg cgggatgtcc ttctcgaacg cctccggcgt cgggtacttc
     1321 ttccgcgtga tctcgtcgag ctgagcctcg gtgtaggggc cccgctcacg gaaaccgggg
     1381 taccagacgt cgaacggctg gaggggccgc ccgagccgct tcgcgacgag cttcccggtc
     1441 tcggcgaacg cgggcgagga gaggaccgac tcgagcatcg ccttcacgcg cgcctcggga
     1501 agctgccggt cctcggcgaa gcggcggtcg atcagcgtcg gggcggccgg cgagtacggg
     1561 tcgacgagac gcgaggcccg gaaggtggcg aggagcttcg cgtagcgcgc gtcgggctcg
     1621 ggggcggccg ccggggcgaa gcccgccggg aggggcggcg cgcccggctc gagggaggtc
     1681 gtcgccttca cctcgttcgt gaaggggttc cagtcgacgt gaggggagtc gatcgccgcg
     1741 gccggaatcg tctgcgtggc gatccgctcc atcgccctcg cgatcgcccg ctgcttcgcg
     1801 aggccgtcct tcccgtcgcc gtagagcgcc ttgatctcgt cccgcaggtt ccagtgggag
     1861 aggaggcgct tgccctgcgg gaagaggcgg cgtcccttct cgtcgaggag gtggtggacc
     1921 cggaggttgt actcggagat gtaccgcgag gcgtcggccg aggcggtcgc gacggcctgg
     1981 ttcacctcgg ccggcacccg cttgccgaac ccctcggcga ggcgcgcctc ggcccactcc
     2041 cgccggctcc acgccggccc cttctcgagc cgctccggga gcgtggtcag cgggaagttg
     2101 aggaggaccg cgaaggcgag gcggttcgag aagaggtcgt cggcgacgtg cgccgacggg
     2161 tcccacgccg ccatcacgtc gtcgaacggg aggatctccc cacggtcgag gtcggagtgc
     2221 tcgcgaaggt cgcggacgac ctcgagcatg tgcccgccga ccgactcgag gacgcgctcc
     2281 atccgcgaga agagggcgtc gagcttcgcg gggtcggaga ggaagtgggt ccgcgccacc
     2341 tcggcgaagg cggcggcgtc gccgtcggag gcgcgccaga gcgcggccgc ctgcacgagc
     2401 ccgcgcgcga cgcggggccg ggaggcctcg ccgtggcgtt cgacgagctc cgcctcgagg
     2461 gacttcagcg cgtcggggga gacggaaggg gcgacggcgg cgctcacggc tccgccgagc
     2521 gagaaggaag ccgcgaggac ggtcgcggcg aggacgatgc gcatcgagag acctccaacc
     2581 ggcgaagtct agccctccgc gcgtcccggg gccggctcag cgcgcgagga ggacgccgat
     2641 cccgagcggc ccggcgaagc tcggcggctc cgcgacgccc cagcgctcgg cgcgctcgag
     2701 gagcctgcgg gcggacgcgc cgtcgccgag gccgtccggg aacggaggcc agagcgtcgc
     2761 gagcgaggtc caggcgctcg gggcgccacg tccgaccgcc ggggtcgcga ggaggagggc
     2821 gtcgccgcgc gcgagcgaaa cggcgggcgc ggcctccgcg cgggtgagcc cgccggcgcc
     2881 gagcgtcccc ggcgcgagga aggagggctc ggggagctgc cgcgcgaacg ggaccggtga
     2941 gggcgccccc cctctgcgag cctggaccgt gcccggcccg agccggacga ccgtcgcctc
     3001 gacgcggccg cccgcggcgg tcgcgacggc gccgacgagg ccgcaggcga gcgcgggcgg
     3061 gacgcggcgg agcgccgggt cgggagcgcg cgtgtgggcg aggacccgcc gcgcgctgcc
     3121 gggagccatc gagccgcgca cccgggtcag ggcgtcggag gagtcgcccg ccgtcagcag
     3181 agcggcgagg agcatctcga ggtgtccctg cgccgtgcgc accgccgcga gcgggtcgga
     3241 ggggctggcg gccgcggcgc gacagaaggt gcgggcggcg acgaccgccg caagccgggc
     3301 cccgagagac gactccgcgc cggtcgcaac cgcgagcgcg atcccgccgg ggaaggcgac
     3361 ggcgaacgac gcggcgagcc ccggcccctc ggcctgcgga cccgcggccg acgcgaacca
     3421 ggccgagacg cccgcgacgt cgacccgctc gcccgcctcg tcgatccgca ggtcgtagga
     3481 ggagaggtcg ggccccgggt cttcccgcgg cgccggaggc ggggccggcc ggggcgccgg
     3541 cggcgcggct tcgggaggcg ggccggcggg agcggcttcg cgcggggcct gccgggcgcc
     3601 ccccttcgcc gtcgcgcgcg gggcgtgcct cagcgagatc accgtcggcg gcgccgcgcg
     3661 gcgcgcctcc tccgcggcga gcctcgtcac ccagtcgacg agccggagca gctcgacgac
     3721 gcgcgcgtgg agcccgaacg ccgcgaacga cgagagctcc cgcaggcttc gctccgtcac
     3781 cgcgttccgc tcgtcctcga gctgcgccgc gagccgctgc ggcagcgtcc gtcgaaggcc
     3841 cgggccgagc gcctcgaacg cgacccccgg ctcggggtcc gggaccggct cggctcggag
     3901 cgcgaagagc ccctcgagaa ggtgcctcgc gaagaggagc cgctcccgca gctcctcgat
     3961 ctcctgccgg gacgccgggc tcctccgcgc gatgctgagg gcgccgacgc gttcggcgac
     4021 ctccccgagg atctcgagga gctccggcag gtcgggcgcg tcgcgtcgat cgtcgtccct
     4081 cacgtctccc ccgcccggat ggtatgagga gccggcggcc gagtccacgg cgccgggcta
     4141 tcctcgcccg tgcgaggcga ccgcatgagc cgaggcccct cccttccgga atcgctcgac
     4201 cgatgggagc gcacgccctc cccggagcgc gccgcggagc tcctcgagtc cgcccggacc
     4261 gcgctctccg cggggaacgc gcccccgtcc ctctggcacc gctggctcga cgcgacgcga
     4321 cggagcgagc acctcctcgc cctcggcgac cgcgaggctc ggcaccgctg ggcggacgcc
     4381 gcgatcggag ccatccacgc gtccggctat gacctcgcgc ggctgctcgc gcagcgcgtc
     4441 gcggcgcatc cggagcgggc cctcctcgtc gacgggccgg cgccgggagc gccgcgctgg
     4501 agctacgcgc acgtggctcg ccgcgccgac gccatcgcgg cggccctcct cgcgctggag
     4561 gccgagccgc gcgtcgcggt cgtcgccgag aacggcgtcg actcggcctg cctcgacctc
     4621 ggatgtctcc tgcacggcct cctcgttgcg cccctctcgc cgcacctgac cgccggcgag
     4681 ctggtcgccg cgttcgacgc gctcggcgtg aacctcgcag tcgtcgcctc cgaggagagc
     4741 ctccggcggg tccgagaggc ggcgcgccgg accgttcgcc cgctccgggt cgtcctcctc
     4801 gacgaggcgg ccgagctcgc ctcggagggg gacgtcgtcc tcggcgaggc gctcgcggcg
     4861 caggccgagg ccgccgtccc gacgctcctc gcgcgcgccc cgcggcgggc gctccacgag
     4921 acgtgcaccg tcctcttcac gtccgggtcg acgggcggcc agaagggggt cgccttctcg
     4981 atggcgaacc tcgtgacgaa gcgcttcgcg cgggccgccg cgctcccctt cgtcggcgag
     5041 gaggagaccc tcctctgctt cctcccgctc taccacacgt tcgggcgcta cctggagctc
     5101 ctcgggatgc tcttctgggg cgggacctac gtcttcgcgg ggaacccgtc gctcgacggc
     5161 ctcgccgccg gcctccgcac ggtcgagccg accggactga tctcgatccc gatccgctgg
     5221 gcgcagctgg agtcccgcgc gcgcgaggcg gcgggcgagg caccctcccc cgagaccgcg
     5281 cgcgccgcgc ttcgttcggt cgtcggaggg cgccttcggt ggggcctctc cgccgccggc
     5341 tacctcgccc cgcaggtctt ccgatacttc caggcgcacg gcgtcgagct ctgctccggg
     5401 ttcgggatga cggaggccac gggcgggatc acgatgacgc ctccgggcga gtacgaggag
     5461 ggaagcgtcg ggctgccgct gccgggcatc cgcgtccgcc tggccgaaca cgacgagctc
     5521 cagatcgaag gcgcctacgt ggcgcgctac ctcgacgagc cggagacggc gccgggcggg
     5581 gagcgctggc tcccgacggg cgacgtcttc cgcgtccggc cgagcgggca cctcgagatc
     5641 gtcgaccgcg tcaaggacat ctacaagaac agtcgcggcc ggacgatcgc cccgcgccgc
     5701 gtcgagtcgc gcttcgaggg ggtcccgggc gtccgccgcg tcttcctcgc cggggacggg
     5761 cgggacgaca acgtcctcct cgtcgtcccc gacgcggacg acccggtcct cgcgggcgac
     5821 ccgcaggggg aatcggcccg cgactacctg cggcggatcg tcgccgccgc gaacgccgac
     5881 ctcgcgccgt acgagagggt cgtcgacttc gccgtcctcg cgcgggattt ctcgctcgag
     5941 gaaggggagc tgacgccgaa gggctcgtac cggaggaagg cgatcgagaa gtcgttcgcc
     6001 ccggtgatcg aggggctcta ccggagtcgc gtcgtgcggc tcgccggcga cggggtcgag
     6061 gtccgagctc cgcgctggct gctccgcgac ctcggcctcc tcgagggcga cctcgtcgtc
     6121 gaagggggcc gcctcgtcga tcgccggcac ggccgctccg ccgccctcgc cgcccgaggc
     6181 gacggtgacg aggtccgggt cgggaacctc tcttaccgct gcgccgggcg gtcggtcgac
     6241 ctcggcctcc tcgcccgcca gccgctcctc tggctgggga accccgagct cgccgcgctc
     6301 ctgccggtga aggacggctg ggacaccccg ctgaccggca gctgcgcgga ggtccttctc
     6361 ccgccgcggg tggaggtgcc ggatgggacc gacgtcccgc tcccggcccc gctcccctcg
     6421 ccccagctca ccgcgctcca caagctctcg gtcgcgctcc tcttcggccc gcccgagtcg
     6481 gcgctcgagg cggtcgcgcg cgccgaggcg ctcctccgcc agggggaccg gcgaacggcc
     6541 gagctcctcc gccggaggct ccaggcgctc gcgcggcacc ccgacgtccg ggtccgctgc
     6601 gaggcgtacc gggtcctcct gctcgacgac ccgctcgtcg actccggaga gatcctcccc
     6661 gcattcctcc gctcgggcct cccgttcctc tccgaggaga gcgtcgcggc gatcgcgcgg
     6721 gccgacctcg gcgaagagcg gctcacggcc ctccgccggc gccttcactc ctaccgcgag
     6781 gcgatgacgg ggccgctgcc cgagacgacc cgcacgacgc tgggggacgt cctcgagctc
     6841 ctgggcgatt tcgcgcggca ccacccggag gaccacgcgg cggtccgcgc ggagctcgct
     6901 tcctgggcga tccaggagcg cgaccccgag ctcgcccgcc gcgccgacgc gctcttcgac
     6961 tccctcggcg tctggcacga ggagacggcc gccgccgcgg cgctccttcc gcccgtctcc
     7021 gagggacgga agaagctctc cttccgcgac gggatgccgg aggaggaggt ccgcactctc
     7081 gaggggctcc tcctcgggac ctcgttcctc cgcgagtcga tcgagctcgc cttcgagacc
     7141 gacccgcccg agctcgccga catccccgag gaagggatct gggtgacgcc ggtccaggcg
     7201 ctccagcgcc accgcctcta ccgtctctcc gtgcggaccg ccacggggaa gcaccacgac
     7261 ctcctcctga cggtccgggg cgacctcgac tcggccgcgg ttcgcgcgac gacgctccgg
     7321 atgatcgcgc tcgccggcca cccgtggggg ccgccggtcg tcccccgcgt cggctcctgc
     7381 cgtcccgacc tcggcgcgct gtctctcgcc tgggtcgacg agctcacgct cgccgagcgt
     7441 cttcgcgacg ccgcggtccc cggacgcgcg cgccccggcg agggtcactc ggtgaagagc
     7501 ctcgtcgtcc gcgcgctcgc cgcgttcttc acggcctggg agcggagcgg ccggctcctc
     7561 gtccccggcc tcgtcacacc ttcgaacgtc gccgtcccgg cggaggacta ccgcgacggg
     7621 gcgcgcctcc tctcgatctc cggcgcccgt acctacaccg ggccgctcga cctcgtcgcg
     7681 ccgatgctcc gcaacgcgtt ccgggagccg gccgcgctgt cgccgcgcct cgccgcggtc
     7741 ctctcgcccg cctggatcgt cgacgccgtc ttcgaggcgc tcggagagga gggcgcccgc
     7801 gcgttcctcg ccgagctgag ggaggcggcc ggcgccccgt cctccgaggc gtggggccgc
     7861 ctcctcgccg cggtcgacgc ggcgctcgcg gccgcccccg gagagcggac gccgctcgcc
     7921 ctcgagaccg ccgtcgcccg ctacgaggcg tgggaggccg agcgccccgg cgtctcgctc
     7981 gccgagcgcg agcggacggc cgagtcgctc ctccgcctct accgcgtcga cacgctcgga
     8041 gaggaggcgc gctaccggct cttccggaag accgtcttcg cccgcagcgc gccgccggtc
     8101 ctcggggcgt tcgacgcgct gctcgccgcg ctccgcgcgc ggcccgggtc gcgcccgacg
     8161 cacctcgtcg agctgcaggg cctccacgcg gcgctctcga cggacgccga gaggcacgcg
     8221 ttcacgcggc tcgtctttcc cgccggccgc tccacccgcc ccgtggaggt gagcgtcgtc
     8281 ggcgacgccg tcaagcacgt cgtcgtcgcg acggagctcg tcgaccggat ggacggcgcc
     8341 tggctcgtcc gcgagccggt cgacgcggcc gaggtgggcc gtctctaccg tctcttcgtg
     8401 agggccggcc tcccccgcgc gttctccccg gaccgccgct acctcgtcgt cctcgacgcg
     8461 tcggagcgga tcgtcggcgg cgtctcgtgg tcgcagctct cccccgacgc ggcgcagctc
     8521 gacggcctcg tcgtcgcgcc gagcgtccgg ggccgcggcc tctcgagcgc gctcctcgac
     8581 gacctctcgg cgcgcctcgc ctcgctcggc gtccgctcgc tcctcgccca cttcgcgttc
     8641 cgcaacatcc cgcttcccgg cttccggctg gaccgccgtc acggcggcct cgtccgcgac
     8701 ctttcggccg gcctcgaggt ggaggcgggg ctggcgtaga gtcgctcgcg tgaagcagct
     8761 gcaggagcgg ctcgagctcc ccacgcccgg gcgcggcctc cacgaggtga ccgaggccgt
     8821 cgccgccgtc gtcgcgcgct ccggcgtcgc gaccggcctc tgcgtcgtct tctgcccgca
     8881 cacctcggcg agactcctcc tgcaggagaa cgccgatccg acggcgcgcg ccgacctcct
     8941 ctcctggctc tcccggctcg ctcccgacgg cgacccgcgc tacgcgcacg acgcggaggg
     9001 ggacgacgac atggccgccc acctgcgaag cgccgtgacc cgttcgagcg agacgatccc
     9061 cgtctccggc ggacgcctcg ccctcgggac gtggcagggg ctcttcctcg ccgagcaccg
     9121 gacgacgccg caccgccggc ggatcgtcgt ccacgtcttc ggagagtgac gtgaggggcg
     9181 cctgcccggc acgcgcgctt cgcgccgtgc tcgcggccct cgcgctcctc gctctcccgg
     9241 ccggcgcggt ctcctccgtc ccggacccgc cgagcgccgc ctcgtcggcg ctcgcggtcg
     9301 cccctggctt cgtcgtcccg gcctcgtacc gctcgcccgc cgacggcgtc gagcgcggct
     9361 acgcgctccg cgtcccccgg acctgggacg ggacgagcct cctccccgcg atcctcctcc
     9421 tccacggaag gggcggaacc gtccgccagt tccagaggac cccatacttc gacgccgcag
     9481 acgcacgcgg cgtcgtcctc gtcttctggg aagggcggat cgcgcccggc ggggccgggg
     9541 tcccctcgac gcactacgtc gacggtgcgg acggcgtccc cgacgagacg gacgtcctcg
     9601 cctgcctcga cgacgccctc gcgcgcgcgc cgatcgacgc cgaccgcgtc gcgctcgccg
     9661 gcttctcgca gggcgggcgc ggcgcgctcg ggattggtct cctcaacccg tcccgcttcg
     9721 ccgccctcgt cgacgccgcc ggcccgaccg acgcgttcca gggacagctc tggtcgccgg
     9781 cattccccga ctacttctcc gccgcgggcg gcccgccgtc gtcgggcggc gaggtcctcg
     9841 cgcgctggca cgagctctcg ccgcgcttcc tcctcccgaa cgcgcggaac ctcttcgtgg
     9901 cggtcctcca cggacaggcc gacgacgtgg tgcccgactc cgcagcgtac ttcccttacc
     9961 gcaactccca ccacgtcacc gtcacgccgg gcttctccga cgcccgcggc cggacgccga
    10021 cgctcgccga gctgcacgcc gacgaccccg ccggctacgc cttctcgacc ggctttcccg
    10081 acgacgtcgg acacgaccag gcccgtctcc tcaccgcgac cgcgctcttc gaagcggcgc
    10141 tcgggaagac gcgccccgcg cgccccgcgc gcgtcgtcgg cgtcgctttc ggcgcgcggg
    10201 agcggacgtt ccactgggcg cgcctcggga ggagggctcc ggcggacggc acccgcgttc
    10261 tcgtcgacgc gcgctccgac ttcccggcga atcgcgtcga cctccgtgtc gaagggcccg
    10321 cccgcgtccg cctcgagctc cccggcgccg gcctcgacgc ctcgcgggct ctccttgtcc
    10381 gcgtcgagac ggcgggcgcg ttcgacctcg cgctcgccgg accgttccct cccgccgtcg
    10441 cggcgacgct cgacggcctg ccgattccgc tcgtgcggac ggccgagggg gtcgtcttcc
    10501 ccggcctcgc gtccgcgacg ccgggttccc tcctcgtcgt cgcgccctct ccggccggac
    10561 cgatcgcgga aggggacctg ctcgtcccgg cgctcgtccg gacggaaggc gccaacggcg
    10621 cccgctacga caccgtcctc gcgctcgcga acctctcgga cgcgccgctt tcgctcgagg
    10681 cgctcctcct cgacggaacg tcggcccccg tccccgtcct ccttcctccc gagtcgagcc
    10741 gcgccgttcc cgccgaagcg ctccgcaccg cgccgggctc gttcgccgct ccgctccggc
    10801 tgcgcgtcgt cgcgggcgag agcgccgcgc tggcctcctc cgcccgcgtc ttcagccggg
    10861 cggacacagg gacgtacggg ctctccttcc ccgtccttcc ggccggggag agcgtcctcg
    10921 cggccggcga gaccgggctc ctcttcggcc cgcgcgatcc ggcggccgag cggctgaacg
    10981 tctcgctctt cgccccgttc ggggcgtcga cggccgaggt caccgtcttc gacgggcggg
    11041 gcctcgccct ccggccccgg cccgtcgcgc tcgccgctct cgcgcgcgtg cagctcgacg
    11101 acctcctgcg cggcgaggcg cccggcgccc acgtccgggt cgcggtcctc tcgggacgcc
    11161 tccaggtcta cggcaccgcc gtctcgaacg gtcggacgaa cgatccctgg cgcgtccccg
    11221 cgcttcgcct cgccggcgcg gcgaggtcct ggaccgtccc cgccgtcgcg tcggccggag
    11281 ggcggaacgg cgcgtacttc cggagcgacc tcttcctcct cgcggccgag gaagcgtccc
    11341 tcgacgcgat gcttctcccg cgcgacggca gcccgccgga aactgcccgc ctcgccgttc
    11401 ccggcggcgc gccgcacgtc gtcgaggacc tcctcgccgt gctcttcccg tcgaaggcac
    11461 ccggcgccgg agcgctcctc ctctcctcga ccttgccgtt ccttcctctc gccgtcaccc
    11521 ggagcgaacc gccgaccggc ccctcttcgc aggacctccc ctgcgtcccg gaggggggcg
    11581 aggtggtccc cggccgtccc gtcgccttcg ccggggtcga cgaatcgtcc tcggcccgct
    11641 cgaacctcgt cctcgtcgcg cccgctgccg ccgcgcgcgt gaggctccgc ctcttcgttc
    11701 cgtcgggacc tctcggcgag cgcgtcgtcg acctccccgc gggccgcgtc gtccagctcg
    11761 actccgtcgc cgcgcgattc accgacgacc ccgtcgaggg agggacgctc ctcgtctcgt
    11821 gcgagagcgg tgccgtcgtc gcgtcggtcg cccggatcga cgccgcgacg aacgatccgg
    11881 ccggcctcgc gccgctgccg gtcgtcgtcc gctgaaggtc aggctctctc gagcttcgcg
    11941 cgttccgtcg cttccagcag gaaaccggcc cggaggaggt agtaccaggc gtggaggagc
    12001 gcgacggcca ctccggcgcg tccgtcgagg aacccgcgct tgaggacgta cgaccggagg
    12061 aactcgagcg ggggccggag gacgaggagg aggacgttcc ctcggccgcc gcgctcgaag
    12121 cgctcgaggg ccgagaggcg cgcgtagagg acgatcttcc ggaccgcgtc ggcgaggtcg
    12181 cggtacgggt ggtggaggag gggcctggag aggtcgcgga ccttgccgtc gacctcgatc
    12241 cactcgtgga cgtgcccgcc ctcagcccga aagccgcgag atcgccgccc gaggcgcagc
    12301 ttccggtcgg gccaccagac cccgtggcgg atcggccggc tgccgaggtg cgagaggcgt
    12361 gacatccgga atccgtccgc gccgtccggt ccggcgacgg cttcgcggat ctccgccgcg
    12421 agctccgcgg tcacggtctc gtccgcgtcc aggacgagca cccagtcgtt cgccgcgagg
    12481 gagatcgccc gattccgcgt ggcgacgtag ccctcccacg gaatcgtctc gacgcgtgcg
    12541 ccggcctcgc gggccagctc ctgcgtcccg tcggtcgagc cggtgtcggc gacgaggacc
    12601 tcgtcggccc aggagacgga ggcgagggag ggggcgagcc ggtcggcctc gtccttcacg
    12661 atgaagacga cggagacggc ggcgcgcatc cctcgattat cctcgacggc gtgaggctcc
    12721 tcctccgcgc gacgaactgg gtcggcgacg tcgtcatctc cctcccggcc cttcgtgccc
    12781 tgcgcgcgca ccaccgcggc gaccacctcg ccgtcctcgc ccgaccctgg gtggccgacc
    12841 tctaccgcct cctgccggaa gttgacgagc tcctcgtcga ggagccgagg gggcgccacg
    12901 ccggacgcga gggacggcgc ctcctcgccg acgagctccg ggagaaggct ttcgaccggg
    12961 cgatcctcct gccgcggagc ttcgcgacgg cccggaccgt ctctcgggcc ggcattcccg
    13021 agcgatgggg attccgcggc gagctccggt cgcccctcct cacccacccc gttccgttct
    13081 ctccccgtcc gggcgagcac gagctgcatc ggcacctccg cctcgtggag gcaacgggcg
    13141 tgccgggtcc ggagatcccc gacgcgacga tcgcggtccc cgacgccgtc cggcgcggcg
    13201 cccgcgccaa gctcgccgag gcggggctcg cctcgggccc cttcgccgcg gcccacgtcg
    13261 cctccttcgc ccacgaagcg aagcgctggc ccgaggagcg gttcgccgcc ctcttcgcgc
    13321 gcctccacga agagcgcggc ctccccgtcg tcctcctcgg cagcgccgcc gaggccggca
    13381 tgaacgcccg cgtcgccgcg ctggccgccc cggcgcgggt cgtcgacctt gcggggagga
    13441 cgtccctcgc cgaggtcctc ggcgtcgtcg cggaggcgga gctcttcgtg gggaacgact
    13501 cgggcctcgc gcacctctcg aacgcggccg gaacgccgac gaccgtcgtc ttcggcccga
    13561 ccgaccccga cgccacccgc ccctgggacg gcccgcgccc cgacgggaag cccgtccggc
    13621 tgcggatcgt ccgcaaccgc gtcccctgcg ccccctgccg cttcacgcgc tgccccctcg
    13681 accacgcctg catgaccggc ctgacgttcg agctccccgc gtaacgcccc gggggtctcg
    13741 cctcgggggt ctggtctaca ctttacagtc tgcgcggcgg gcgaggctgc gccgcgagcc
    13801 ccggcctccg caccggcttc ccgtcaggcg tcgagcggcg cgcagaggag gtggaggacg
    13861 gcgagcgtca cctcctggac gtgcgcggtc tcggccgtcg gcacgacgag gccgtggtcc
    13921 cacctcgccg cttcggcggc gccgcgcgtc ccggtgacga ggatcgtcac catgccgcgc
    13981 gcgcgggcgg cctcgagcgc cttgacgacg ctcgggctcg tgcccgacgt ggagagcgcg
    14041 agggccacgt cgcccgggcg gccgagcgcc tcgacctgcc gcgcgaagac gtagtcgtag
    14101 gagtagtcgt tcgcacaggc ggtcaggatc gccccgtcgc agacgagggc gacggcgggc
    14161 aggcccggcc ggtcgccctt gtagcggacg acgagctcgg cggcgaagtg ctgggcctgg
    14221 gttgcgctcc cgccgttgcc gaacgcgagg gccttgttcc ccgcccggac cgccgtcgcg
    14281 accgcggccg ccgccgcttc gatcgccggc ttcatcgctc ctcgcgcggc gagggccgtc
    14341 tcggcgaggg ccgagagatg cgcgtcgatc acggcgcctc cttcgtcttc gacatcttcg
    14401 cgaccattga ggtcgtcgag cgcccgggca cgagcgggac gagcgcgacc tcgccgccgt
    14461 aggagcggac gaacggcgcg ccgacgaccg actcctcggt gtagtcgccc cccttcacga
    14521 ggacctgggg acggatcgcg cggatgagcg cctcgggcgt gtcctcctcg aagaggacga
    14581 cgaagtcgac ggcgtcgagg ccgccgagga cctgcgcgcg gtccgtctcg gagaggatcg
    14641 gccgctcggg tcctttcagc ctcttcaccg aagcgtcgga gttgaggcct acgacgagga
    14701 cgtcgccgag ctgcttcgcc gctccgagga gcgtgacgtg gccggggtgg aggaggtcga
    14761 agcagccgtt cgtgaagacg acgcgccggt gcgcgcgccg gagcatctgc gcgacggcgc
    14821 cgacgtggcc ccgctcgagg agcttgtcgg ccggcgccga ggtcagcccg tcgaggaggt
    14881 cggcccagcg gacggacgcg gtccccgtcc gcccgacgac gacgcccgcc gcgcggttcg
    14941 cgacctcggc cgcgtcggcg agcgggaggc ccgcgccgag cgcgacgccg agcgtggcga
    15001 ggacggtgtc ccccgcgccc gtgacgtcga agacctcgcg ggcgcgcgcg gcgatccggt
    15061 gcggcgcgcc ccccttcggc aggaggagca tcccctcctc ggagagcttg acgaggatcg
    15121 cgtcgaggcc gaggtcgtcg cgcagccgcg tcgcgaggcg cacgacggcg gcggcgtcgc
    15181 gcgcgctctc gcccgtcacc gtctcgagct ccttgcggtt cggcgtgacg cacgtggcgc
    15241 cgcggtagcg gccgtagtcc ttccccttcg ggtcgacgag ggccgggacg ccccgccggc
    15301 gcgcctcggc gaggagcgtt tccatgaggc gcggcgtgag cgtccccttc gcgtagtcgg
    15361 aaaggagaag gacgtcggcc gactcgagcg cggcgagggc cgcgccgatc agctcctcct
    15421 cggccccctt cgagaggacg gcggggagct cctggtcgac gcgaagaagc tgccggccgt
    15481 cggcgaccat ccgccgcttg acgggcgtcg tgcggagcgg gtcgacgacg acgcgcccct
    15541 ccccgcccgg aagcgagccg aggtgacggg cgatccaggc cccttcctcc tccgggccga
    15601 cggcccccag cgcgaccgtc cgggcgccga gggccgcgag gttctcgacg acgttccccg
    15661 ctcccccggc ccgccggcac atcgacgaga gcgcgaggag cggcaccggc gcctccggtg
    15721 cgacccgcga gacgtcgccc agccacgtct cgtcgagcat caggtcgccc acgacgaccg
    15781 ccttcacgct cggcgccgcg gcggcgagag cggccagctc gcgtggcgtc atcgcgtcct
    15841 ccggctcact tctactcctc cagccccagg acgagcgcct gggcgaggag cggcaggacg
    15901 atctcgtggt gcccggtgat cgcgtacgac gccccgccgg agaggacggg acgcttcagg
    15961 acgttctcgt gcgggcggta gtgccggttc atgtcgaggt cgacggtcgt gaagttcctc
    16021 acgtcgtggc cgaggttgcg cgccatcgag agggccttca ggaagacttc cggcatgatg
    16081 acggccgacc cggcgttgat ccagaccccg ccgtcgccga gcccggcgat cacgcgccgg
    16141 atcgtcgtga agtcgcggat cgacatctcg ccgacgagcg cgccgtccat cccgggatgc
    16201 tggacgaggg tgtcggtccc gagcgcgacg tggacggtga gcgggacgtc cgccttcgcc
    16261 gccgcggcga agagggagag gtggcggaac gggaggcggg cccggaggat cccctcgccg
    16321 agggcgcggc cgtagccgac gccgttcctc cggccggcgc ggagggcccg gaagaccccg
    16381 acgcccgtct cgcggctcat cccgaactcg ccggtctcga ggaccggccc gacgtcctcc
    16441 gaggtcttcc cgtggaaggc gacctcgaag tcgtggatgc cgctcgcgcc gttcgtcgcg
    16501 agcgccgtga cgaagccgcg ctcgacgagg tcgatcagga tggggccgag gcccgtcttc
    16561 aggacgtggg cgccgaacgc gacgacgacc ggcttcccct tccggcgtgc cgcgtggatc
    16621 gcggagacga ccttccggag cgactcggcg gcgaggatcc gcggcaggcc gttccagaag
    16681 tcggcgaaag caccgctcgt cgagggcttc gccgcgaagt cgtcggcctt caccttgaag
    16741 gaccgcgtcg cgatcgggaa ggtcgtcacc gtctcgcgcg ggcgtcggcg tttcgtcgtc
    16801 gtcatccgtt cttcccttcc gccgccgcgc ggagcctgcg gaggccgtcg gcgagccccc
    16861 cggccaggat cacccgaccg cctccctttt cgcgcacgcg cgcccggtgc tcccggccgt
    16921 gccccgagag gaggtgaacc gcgatcgcgc cgagcgcgag ccccgcgccg acgtcgtccc
    16981 acttgtcgcc gacgacggcc gaccgctccg cggcgagccc gtgccggcgg agcgcccttc
    17041 ggtggaggag cgtgcccggc ttccggcagc ggcaggcgac ggcgtactcc ttcacccgcc
    17101 cctcggggtg gtgcgggcag acgagccacc cggcgatcgg cacgccgcgc gcggcgagcc
    17161 gccgctcgac ctcggcgttc acggcgctca cctcgtccca gcgcgtcagc ccccgtgcga
    17221 cgccggactg gttcgtcacg acgaagagac tcgtcccctc cgatgcgaag cgggcgagga
    17281 gcggcgcggc gcccggcgcg agccgcaccc ccttcgggtc ggcgaggaag ccgcgctcgg
    17341 cgatgagcgt cccgtcgcgg tcgaggaaga gggcgtcgag gaggggccgg cgcttcactt
    17401 tctgccgacg gccttccgga tcgcctccgt gaacgaggcc tcggtctgga gccccgagag
    17461 gcgggccgcg atcttcccgt cgcggccgta gaccagtgtg tacggcagcg agccgtccca
    17521 ggtccggtcg accgtgtcga tgaagtcctg cgggtcgcgc gatttcgcga gccagcagac
    17581 gaacgggacc ttctgctcct tcaggaactt cgggacctgt gaagggcccg tcttctgcga
    17641 gtcgagcgag acgagcgtga ccgcgaggtc gcgggaaggg aacttcttcg ccgcggcgac
    17701 gaggtacggc atctcctccc ggcacggctc gcaccaggtg gcccagaagt tcacgacgag
    17761 gacccgtccc ttcttgccgg cgacgacgcg cgcggagtag tcgcgcggga ggaggtgctt
    17821 caccggctcg gccgacgcgg aggctgctcc ggcgaggaag acggccgcca ggacggcggc
    17881 ggccgtcttc ctcgtgcccg tgggggactc ggttcccgcc actttcagac gcgcttgatc
    17941 gtgcacccga aggccttcgt ctcggctgcc gggacgggct tgccggccag gatcgcgtcg
    18001 agcgcgttcc ggaggtccgg cgacgtcacg ttcttcgggt cctcgtgggt ctcgtcgatc
    18061 cgcccgtggt agaggagcgt ccccgcaggg ctcaggacgt agacctcggg ggtcttcttc
    18121 gcgccgtaga ggtcggcgac cctgttcccc tcgtccttca ggaccgggaa ggcgaagccg
    18181 ttcttcttcg cgtcctcggc gacctcggcc gcgggctccg tcttgttcga gttgatcgtc
    18241 acgaaggtga tccccttcgc ggcgtactcc ttccccatcg ccgcgacgcg ctccttgtag
    18301 cccttcgcgt aggggcattt cgtcgcgtcg aagatgacga cgacggcctt gctcttgccg
    18361 agcaggtcct tcagggcgac ggccgccccg ccggcggcgg gagggagcgt gaagtcgggg
    18421 gcggcggagc cgatcctcgg cgcggcaggc ggctctgcgg gc
//
LOCUS       DAOTEZ010000022        18327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2031_length_18327_cov_6.637971, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000022 DAOTEZ010000000
VERSION     DAOTEZ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 18327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18327
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2031_length_18327_cov_6.637971"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="P5164_02075"
     CDS             complement(<1..756)
                     /locus_tag="P5164_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HRY42709.1"
                     /translation="MAASGNEARGTIFNVQRGSFHDGPGIRTTVFLKGCPLRCPWCHN
                     PEGIAFAPEVLWSAGRCLSCGSCAAACPRPAGTLPAGATLGSDGCSACRGCVEACPAA
                     AREVAGREVTVAEVVAEALRDRAVYEESGGGVTFSGGEPLAQPAFLLAALAACKAEGL
                     HVAVDTCGFAARVAVLRASARADLLLWDVKTLDPKRHRELTGVPLEPILENLDAVSRT
                     GVRIWLRLPVIPGVNDDAGSLAGAVALAERTRGV"
     gene            827..1588
                     /locus_tag="P5164_02080"
     CDS             827..1588
                     /locus_tag="P5164_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010042365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRY42710.1"
                     /translation="MPSAHRPRRTPPAPPDALGTLLAAAGFALDLFDGLPDVVFFAKD
                     AAGCYRAVNDTLARRLGKRTKRDLLGRTARDLFPAPLGERYLAQDLAVCRTGRPVEDL
                     LELHLYPDGREGWCLTTKLPLRDGAGRVVGLAGTSRDVRPPDALDGSVEGLAGVLEAL
                     HERPGDPHRVEELSRRAGLSPARFSRRIRALFGLSAAQLVVKVRIDAARRMLLDRKTP
                     IARIALACGYCDQSAFTRQFKAVTGLTPAQYRGSP"
     gene            1620..2222
                     /locus_tag="P5164_02085"
     CDS             1620..2222
                     /locus_tag="P5164_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42711.1"
                     /translation="MDLARALDRIEEIHAQVAKGEVFRGYRPVPVAIAGAVGLLAGWL
                     QPSLVPEGDAPRFLGYWIAVALGNVAVNAAVIAAGYARCGDAFARRHTRRVVGQFVPC
                     LLAGAAVTLGLPAAGPGLVRILPGLWALLFGLGVFASRPYLARATGWVALFFVAAGTG
                     LLLFPPATYAALGLAHGAVFGAGLLGAAVVLRLDLPREER"
     gene            2219..2602
                     /locus_tag="P5164_02090"
     CDS             2219..2602
                     /locus_tag="P5164_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRY42712.1"
                     /translation="MSGDATEGRFAYEGLDRVLHEKARLGILTSLATRPEGLLFGDLK
                     RLCALTDGNLNRHLEVLQEAGLVEVWKRFEGRRPQTLVRLSAEGRRRFLDYLGELEKV
                     LKDAKKARALEGAEPGPLKAGWNPA"
     gene            2681..3526
                     /gene="uppS"
                     /locus_tag="P5164_02095"
     CDS             2681..3526
                     /gene="uppS"
                     /locus_tag="P5164_02095"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020093610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HRY42713.1"
                     /translation="MRSDGTVGSDGTVGSDGTVGSDGTVGSGARDGAGGAAALPRHVA
                     IIMDGNGRWATRRGLPRAVGHRAGAKAVRRVVEASRRRGVRTLTLYAFSSDNWRRPAA
                     EVEALMGLFERYLAEEARRCVENGIRLRVIGRRDRLGAPLVAAIDAAEARTAGGTAME
                     LRIAVDYSGRGAILAAIPLLPAGPEPTREAFLAAIARAGHGGAPEVDLLVRTGGERRT
                     SDFLLWESAYAELFFSDRLWPDVGASDLDEALAWYAGRERRFGGLPAPIPAAEGGSRE
                     ASHAA"
     gene            3504..5024
                     /locus_tag="P5164_02100"
     CDS             3504..5024
                     /locus_tag="P5164_02100"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY42714.1"
                     /translation="MKLRTPPELRLAPRLDPELATGAPVPRRALLLNPFYPKDPHASF
                     GKHVLTPTLALTSVAGATPPGWEVALWDENLLQGPPPHEPFPSVVGITVHLTFARRAY
                     ELARWYRERGALVVLGGLHVASCPDEAAPHADALAIGDGVPLWPRILADAERGALEPV
                     YRAGFAGPYRDAPPPRRDLLPRASFLTTASLMATRGCHNRCDFCWLSTRGVKMPGSVK
                     EPAQVVDEWLATGEPYAVFLDNNLGSDPEHLRRLCRALAPLERIWSAAVSIDVTDDPR
                     LVREMALAGCTGVFVGLETLNDPNLADARKRTPPAADYARRVGILHDNGIQVNGSFVL
                     GFDHDGPDVFERTVAWIEANRLECATFHVLTPYPGTPLFRRMEAEGRLLHRDWSLYDT
                     AHVVFRPARMSPERLAEGYTWCYEALFSAGSIWRRRPLDPRAVAPYLAMSLLYKRANP
                     LWTFLIRRRLVAAAWRPLVEATRRRHLVFRRRLAMREVSAAPASRQGETLALFPGA"
     gene            complement(5032..5730)
                     /locus_tag="P5164_02105"
     CDS             complement(5032..5730)
                     /locus_tag="P5164_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42715.1"
                     /translation="MTAEGHVCPRCGIRFDADEEGVFANCPACGSLLHLGGVAEIAHQ
                     RLVPVLDEAAALGHLARWLRGREVLADPKEAATRLLGFPFRLLPGDALIPAAPLLASN
                     LGAFRAPAGDRVAFREGEAGPAEIVPASVPPAAIVVDEVTRRAAKLLHVPFREVCFRL
                     WGRDDTIWFDAATGQALDFDLPPTSERRLDVTYALLLLALLVSHACRVSMGNVAEKVS
                     GFVAPAGCLRPPAA"
     gene            complement(5810..6196)
                     /locus_tag="P5164_02110"
     CDS             complement(5810..6196)
                     /locus_tag="P5164_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HRY42716.1"
                     /translation="MAIRVVRLGSPRGKGEGLRLGTVRRPPRGVPKAEFASRDFYDVW
                     LPDLAPGEELVKAAQAAADERAWKAFERKYRAEMGAPHAARLLDLLAALSHRTSLAVG
                     CYCADEARCHRSILRALLAERGAEVA"
     gene            complement(6208..7659)
                     /locus_tag="P5164_02115"
     CDS             complement(6208..7659)
                     /locus_tag="P5164_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRY42717.1"
                     /translation="MRETEPFFFGRTFDDFLFRPQRSPVASRRAVDLSMPLVRGLDLG
                     LPIVGANMDTVVGEEMARALALEGAFGFLHRSSPIEAQAARVRYVKTRHSYVIEKPLV
                     LPRTATIAEVRRLIEKHRSSGILIEETAGSGLLAGILSHRDLPHGADANERRVEELMT
                     PVARLVTRPPSVSLEEAEKAMWERRVEKLPLVDDAGRIRGLVTMRDLHLFKKKPWSVK
                     DERGRLRVGAAIGAAGDYLERAAALAEAEVDVVLMDVAHAHSEILAKAVPAFRSRFPG
                     IPLVVGNVATAEGARFLSDLGAAAVKVGVGPGRGCRTRLETGAGVPQLQAIREAFLGT
                     EGKVPIIADGGIRNDKDAFLALACGASTVMLGSLLSGTDESPGIVVDDPATRQKAKLY
                     RGMTSPEAVVDGSTDAGVADALATPAEGQSVRVPYVGSVVDVLARIAGHLRSAVSYAG
                     ESTLEAAHRKIAADPGRYLVPLSSAARAESFDR"
     gene            7817..10807
                     /locus_tag="P5164_02120"
     CDS             7817..10807
                     /locus_tag="P5164_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRY42718.1"
                     /translation="MRRRGLGAVPACIVLCGAVFIARNAPALDVARPLSQAQVDVWRT
                     ENGLPQDTVTSILRTREGHLWFGTYDGLVRFDGARFTVFDGKTTPLLATGSAFALMED
                     RHGAIWVGRSENVLKYENGAFTQVLGADELGQGTVWALAEAPDGTVWAGAGRGLVRWT
                     AGKATLLTKENGLPPARLRTLCVDTDGALWVGTSGAGLARLKDGRVTRWTAKDGFPSD
                     QIVGVLPDPSGGIWVATAGAGLVRIVGDSRRVWTTADGLPTDQLTALALDAAGSLWIG
                     TWGSGICRFHRGTFSVLAAPPLSNDKIWWLLPDEEGFVWVGTWVGGLNRLRDRSFPSF
                     GVPEGLSNDNVRSVLHARDGSVWLATAGGGLNRLRDGRLDVIRKADGLPSDEVSALCE
                     DRTGVLWVGTYTSGLVKLRGGRIEAFGKDDGLPGDDVRVIFEDREGTLWVATTTGVAT
                     SRDGRRFERVATPEWVSLNAVVCMLEDRQGALWFGTSGDGLVRRDDTGFRVLTTSDGL
                     VSDRIAALHEDDEGTLWIGSAWSGINRLKDGRITAIRPENGLAEGRAQTLIPDRFGGL
                     WLTGNKGFQRLLKKELNEVADGLRRVVRPLSFGLADDLRSASFASGQQPAGSVDSDGR
                     IWLPSYRGIVVVDPARIPPPPPAPGARVEEVVVDGSLRAARRELKIEPARRLLEVRYA
                     ATTLRPPELVGFRFRLDGFDDDWHEVGTRRAAYYTALPPGRYRFRVQSRVGDGAWGQE
                     AEPLALVLEPAFHQTSWFRLLAALLVLGGAGLLLGSRTARLKRRHDELERLVGERTDA
                     LREANERLSELSFSDALTGIPNRRRLDEALNAEWKRAVRFGHPLSLVLADIDFFKRYN
                     DALGHPAGDRCLVEVARVLQANARRAGDVAARFGGEEFALLLPATSGTDAAAVAERTR
                     AALEALALPHPEGAAPVVTASFGVATFVPESDGDPDRLLATADAALYRAKREGRNRVV
                     SATPRGGRPPEAGRSGEHPLPKAL"
     gene            10857..13463
                     /locus_tag="P5164_02125"
     CDS             10857..13463
                     /locus_tag="P5164_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HRY42719.1"
                     /translation="MTIPEKPSVTPLADRVPPRGTTDGDEILGLFLDWVADTGLTLYP
                     AQEEALLEIMAGKHVILGTPTGSGKSLVALGLHFKALCEGRVSYYTSPIKALASEKFF
                     ALCNELGAENVGMRTGDASINADGLVVCCTAEVLSNMALRLGEELDAPYVVMDEFHYY
                     ADKERGVAWQVPLLVLPGTQFLLMSATLGDTSAIAERLTHDTGREVAFVTSEERPVPL
                     DFAYAETPLHQTIEKLLEAGKSPVYVVNFTQRECAELAQALTSVRIGTKEEREAIREV
                     VAGRRLVTPYGKEFRRFLSFGVGVHHAGLLPRYRLLVEQLAQRGLLHVICGTDTLGVG
                     VNIPIRTVLFTKLAKFDGAKVGLLTVREFKQIAGRAGRRGFDTQGSVVAQAPEHIVEK
                     KQATKKSDASGKKVTKTFRGPAKGEISWTEETFQRLITRPPETLKSRFHLTHGMVLDL
                     IQRDADTNRDAPPPERNFSALRDLVARCHEEDATKARLLKHAALLVRSLYRAGIVGMS
                     VEETGFRRVVVADDLQWDFSLHQALSIYAVTALERLDPASPTYALDVVTVVESILENP
                     DIVLRKQTDRAKGRLVEELKAEGVPYEERMAKLEEVTYPQPLADFLFGAFEAFREAHP
                     WAGGTEPKPKSIGREMFETFAGFSDYVKSYGLERSEGVLLRYLSQLYRTLDQTVPEPA
                     KTDELWDVLGFFRALVGETDASLLEEWESLRHPEELLEAKAEPEKPKQAAWLRELLSD
                     PRVFAARVRSEMHLLVRALSRQDWEDAAERVWQPAENAEGAAEAAWTPERFEAALAPF
                     FEEHAELLAGPESRLHQWTSLRPAGPRQWTVTQTLLDPEEENAWAVFAGIDLGDETAP
                     DGPILRLTRIGT"
     gene            complement(13513..14415)
                     /locus_tag="P5164_02130"
     CDS             complement(13513..14415)
                     /locus_tag="P5164_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017136526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HRY42720.1"
                     /translation="MGERATGEGHVSFRGFRTWYRVVGEGEAPGRDPVLVLHGGPGTG
                     HDYLEPLSALAASGRRVVFYDQLGCGRSDHPHDPSLWDVGLFVDEVAAVRAALDLPRV
                     HLLGQSWGGMLALETALAKHPGLVSLVLANSLASVPLWISETNRLRDELPPEVRATLS
                     RHEADDTTGSTEYQGAVLTFYRRHVCRLDPWPECLERTFANLAEDPEVYNVMFGPSEF
                     HATGVLKEWDVTPRLGEIRLPTLVLSGRFDEATPAVVEALQRGIHGSERVVFEESAHM
                     PHLEEPESFLRVVQDFLERAETRP"
     gene            complement(14473..16029)
                     /locus_tag="P5164_02135"
     CDS             complement(14473..16029)
                     /locus_tag="P5164_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRY42721.1"
                     /translation="MRDRTRRHPLVSLALGAALLVFPPLLAETPVPSGRSMTDVLSAL
                     EAFALEARGAEGIPGMAYAVVKDDAVVFSKALGERKAGEAAPVTTSTLFEIGSTSKAF
                     TAALLGMAVEEGLISWDTRVVDVLPRFRMHDPWVTREMRIADLVSQRSGMPPYSLDWM
                     AFIGFGRDEIQQALVHVEPVSSFRNTFGYVNTLWLVAAEVLEKVTGQRWEDAVHLRVL
                     GPLGMRETTVDPEVVGMAGDVASGHLRQADGGLRPIPPDWPFRGWIDTYGPAGSIRST
                     VLDMSRWARLHLARGTFEGEPHLAPGTVDLLHAPRTLAFARDGSSGSYAMGWISESRR
                     AGRIVWHNGGTSGQHSIVALYPAANAALVVLTNSSDNQVPERMMSKLFDLLFTPPAPT
                     LARRAQLLAEEEPLPSFRIAAAPKPALLAPLPSLPLARYVGVYENPAYGRFTVTEKGG
                     GLVMTMGPWKIEGVLTHYSGNVWALSLPSYPEMAAGVTFAVPAGRPAERLVVDMCADA
                     GGGIFGRVDR"
     gene            16205..17044
                     /locus_tag="P5164_02140"
     CDS             16205..17044
                     /locus_tag="P5164_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY42722.1"
                     /translation="MTLACDVALAARIERAEALLLADAVARVALRVGERGDVLSRPLA
                     GGIAAFAEPGSPLNKVAGLGFDGALPETELEEVEKGFARRECPVRVELSSLADPALGA
                     LLTRRGYVLEGFEDVLGLALPTRGFPPIPEDVKVRPSGPEELELWLDVTVAGFLSPDT
                     EGVPSNESFEEAVLRRVIRDFAQAEGMTRYLAERGGAPAGGGSVRIADGLAQLCGAAT
                     LPAHRRRGIQTALLSERLAAAGRAGCDLAVVTTQPGSKSQENARRHGFALLYTRAVLV
                     RTA"
     gene            17313..17618
                     /locus_tag="P5164_02145"
     CDS             17313..17618
                     /locus_tag="P5164_02145"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRY42723.1"
                     /translation="MPAVPGLRRGLSALYGERLKGVYVFGSRARTDAAMDSDLDVLVV
                     LDRIDSYAEELERTSALTAGLSLALGFTVSRVFAAEADWETAKSPFLAGVRADAVSA"
     gene            17615..18001
                     /locus_tag="P5164_02150"
     CDS             17615..18001
                     /locus_tag="P5164_02150"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HRY42724.1"
                     /translation="MNEASAAFLAKAERALDAAETLLREGHAEFASGRAYYAMFYAAE
                     AMLAEAGEAFRKHSGVHAAFGRRFAQGGELDPKFHRWLLDAHDARLADDYGFESALTN
                     EDVRTMLDRAAEFLGATRSWLAAWPS"
     gene            complement(18062..>18327)
                     /locus_tag="P5164_02155"
     CDS             complement(18062..>18327)
                     /locus_tag="P5164_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003595012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper-transporting ATPase"
                     /protein_id="HRY42725.1"
                     /translation="ESASVTLLHGDLAGLVRARRLGRATMRNVRQNLAWAFGYNLLGI
                     PLAAGLLYPVFGWLLSPMIAGVAMSLSSVSVIVNSLRLRRLEL"
BASE COUNT         2505 a         6631 c         6704 g         2487 t
ORIGIN      
        1 gacgccgcgg gtcctctccg cgagggcgac cgcgcccgcg aggctcccgg cgtcgtcgtt
       61 gacgccgggg atcacgggga gccggagcca gatccgaacg cccgtccggc tcacggcatc
      121 gaggttctcg aggatcggct cgagcggcac gccggtcagc tcccggtgcc gcttcgggtc
      181 gagcgtcttg acgtcccaga ggaggaggtc cgctcgggcg gacgccctga ggacggccac
      241 gcgcgcggcg aagccgcacg tgtcgacggc gacgtggagc ccctccgcct tgcaggccgc
      301 gagcgccgcg aggaggaacg ccggctgggc gagcggctcg ccgccggaga aggtgacgcc
      361 gccccccgac tcctcgtaga cggcccggtc gcggagggcc tcggcgacga cctccgccac
      421 ggtcacctcc cgtccggcca cctcgcgcgc cgccgcaggg caggcctcga cgcagccgcg
      481 gcaggccgag catccgtcac tcccgagcgt cgccccggcg gggagagtcc cggcgggccg
      541 cgggcaggcg gccgcgcagc tcccgcacga aaggcaccgt ccggcgctcc agaggacttc
      601 cggtgcgaag gcgatcccct cggggttgtg gcaccagggg cagcggagcg ggcacccttt
      661 caggaagacg gtcgtccgga tgccgggacc gtcgtggaac gagcccctct gtacgttgaa
      721 gatcgtcccg cgggcctcgt tcccggatgc ggccatcggt ccctcgcccg gagatcgtgc
      781 cggagggcgg ccgacggcgt cttgtacgat tccgccgggt ctcgcagtgc cttccgcaca
      841 tcgcccccgc cgcacgccgc ccgcgccgcc cgacgcgctc ggcacgctcc tcgcggccgc
      901 cggcttcgcg ctcgacctct tcgacggact ccccgacgtc gtcttcttcg cgaaggacgc
      961 tgcggggtgc taccgggcgg tgaacgacac gctcgcgcgg aggctcggaa agcggacgaa
     1021 gcgggacctc ctcggccgga cggcccgcga cctcttcccc gctccgctcg gcgagcgcta
     1081 cctcgcgcag gacctcgccg tctgccggac gggtcgcccc gtcgaggacc tcctcgagct
     1141 tcacctctac ccggacggac gcgagggatg gtgcctgacg acgaagctcc cgctgcggga
     1201 cggggcgggg cgcgtcgtcg gcctcgcggg gacctcgcgc gacgtgaggc ccccggacgc
     1261 cctcgacggc tccgtcgagg ggctggcggg agtcctcgag gccctccacg agcgcccggg
     1321 cgacccgcac cgggtagagg agctctcccg gagggcggga ctctcgcccg cccgcttctc
     1381 ccgtcgcatc cgcgcgctct tcggcctgtc ggccgcgcag ctcgtcgtca aggtgcggat
     1441 cgacgcggcc cggcggatgc tcctcgaccg gaagaccccg atcgcccgga tcgccctcgc
     1501 ctgcggctac tgcgaccaga gcgccttcac ccgccagttc aaggcggtga cgggcctgac
     1561 gccggcgcag tatcgtggtt ccccttgaca actctgtaat gcagagtaaa ttctccggca
     1621 tggacctcgc acgcgccctc gatcggatcg aggagattca cgcccaggtg gcgaaggggg
     1681 aggtcttccg cggctaccgg ccggtgccgg tcgcgatcgc gggggccgtc gggctcctcg
     1741 ccggctggct tcagccgtcg ctcgtccccg agggggacgc gccgcgcttc ctcgggtact
     1801 ggatcgccgt cgctctcggg aacgtcgcgg tcaacgccgc ggtcatcgcc gccggctacg
     1861 cccgttgcgg ggacgccttc gcccgccggc acacccgccg ggtcgtcggg cagttcgtgc
     1921 cgtgcctcct cgcgggggcg gccgtgacgc tcggccttcc ggcggcgggc cccgggctcg
     1981 tccggatcct ccccggcctc tgggcgctcc tcttcggcct cggcgtcttc gcctcgcggc
     2041 cgtacctcgc ccgcgcgacg ggctgggtcg cgctcttctt cgtcgccgcc gggacgggcc
     2101 tcctcctctt cccccccgcg acctacgcgg cgctcggcct cgcccacggc gccgtcttcg
     2161 gcgccggtct cctcggggcg gccgtcgtcc tccgcctcga cctcccgagg gaggagcggt
     2221 gagcggcgac gcgaccgagg gccgcttcgc ctacgagggg ctcgaccgcg tcctccacga
     2281 gaaggcgcgg ctcggcatcc tgacctcgct cgcgacgcgc cccgaggggc tcctcttcgg
     2341 cgacctcaag cggctctgcg cgctgacgga cggcaacctg aaccggcacc tcgaggtgct
     2401 ccaggaggcg ggcctcgtcg aggtctggaa gaggttcgag gggcgcaggc cgcagacgct
     2461 cgtgaggctg agcgccgagg ggcgccggcg cttcctcgac tacctcggcg agctcgagaa
     2521 ggtgctcaag gacgcgaaga aggcccgcgc cctcgaggga gccgagccgg ggccgctgaa
     2581 ggcgggctgg aacccggcct gaggcgggag ggacggcgac gaagggtttt gtgcgccttg
     2641 ttgctttgta atgccgagta cttttctgga ggaagatacg atgaggtccg acggtacggt
     2701 ggggtccgac ggtacggtgg ggtccgacgg cacggtgggg tccgacggca cggtggggtc
     2761 cggggctcgg gacggggcgg gcggcgcggc ggcgctcccg cggcacgtcg cgatcatcat
     2821 ggacggcaac gggcgctggg cgacgcgccg cggcctgccg cgggccgtcg ggcaccgcgc
     2881 cggcgcgaag gccgtccggc gcgtcgtcga ggcgtcccgg cgccgcggcg tccggacgct
     2941 cacgctctac gccttctcct ccgacaactg gcgccgcccg gccgccgagg tcgaggcgct
     3001 catgggcctc ttcgagcggt acctcgccga ggaggcgcgc cgctgcgtgg agaacgggat
     3061 ccgcctccgg gtcatcggcc ggcgcgaccg cctcggcgcc ccgctcgtcg cggcgatcga
     3121 cgccgccgag gcccggacgg cgggcggcac ggcgatggag ctccggatcg cggtcgacta
     3181 ctcgggacgg ggcgccatcc tcgcggcgat cccgctcctc ccggccggcc cggagccgac
     3241 gcgcgaggcg ttccttgcgg cgatcgcgcg ggcggggcac ggcggagcgc ccgaggtcga
     3301 cctcctcgtg aggaccggcg gcgagcggcg gacgagcgac ttcctcctct gggagtccgc
     3361 gtacgccgag ctcttcttca gcgaccgcct ctggcccgac gtcggcgcgt ccgacctcga
     3421 cgaggccctc gcctggtacg ccggccggga gcgccgcttc ggcggcctcc cggccccgat
     3481 tcccgccgcg gaaggaggct cccgtgaagc ttcgcacgcc gcctgagctg cgcctcgccc
     3541 ctcgcctcga cccggagctc gcaaccggcg cgccggtgcc gcgccgcgcc ctcctcctga
     3601 accccttcta cccgaaggac ccgcacgcga gcttcggcaa gcacgtcctg acgccgacgc
     3661 tcgcgctgac gagcgtcgcg ggcgcgacgc cgcccggctg ggaggtcgcc ctctgggacg
     3721 agaacctcct ccaggggccg ccgccgcacg agccgttccc gtccgtcgtc ggcatcacgg
     3781 tccacctgac cttcgcccgg cgcgcctacg agcttgcccg ctggtaccgc gagcgcggcg
     3841 cgctcgtcgt cctgggcggg ctccacgtcg cctcctgccc ggacgaggcg gcgccgcacg
     3901 ccgacgccct cgcgatcggc gacggagttc ccctctggcc gcgcatcctc gcggacgcgg
     3961 agcgcggcgc cctcgagccc gtctaccgcg ccggcttcgc cgggccgtac cgcgacgccc
     4021 cgcccccgcg gcgcgacctc ctcccgcggg cgtcgttcct gacgacggcg agcctcatgg
     4081 cgacgcgcgg ctgccacaac cggtgcgact tctgctggct gtcgacgagg ggcgtgaaga
     4141 tgcccgggag cgtgaaggag ccggcgcagg tcgtcgacga gtggctcgcg acgggcgagc
     4201 cgtatgccgt cttcctcgac aacaacctcg gctccgaccc ggagcacctg cgccgcctct
     4261 gccgggcgct cgcgccgctc gagcggatct ggagcgcggc cgtctcgatc gacgtcaccg
     4321 acgacccgcg cctcgtccgc gagatggcgc tcgccggctg cacgggggtc ttcgtcggcc
     4381 tcgagacgct gaacgacccg aacctcgccg acgcgcggaa gaggacgccg ccggcggccg
     4441 actacgcgcg ccgcgtcggg atcctccacg acaacgggat ccaggtgaac gggagcttcg
     4501 tcctcggctt cgaccacgac ggcccggacg tcttcgagag gacggtcgcc tggatcgagg
     4561 cgaaccgcct cgagtgcgcg acgttccacg tcctgacgcc ctatcccggc acgcccctct
     4621 tccgcaggat ggaggccgag gggcgcctcc tccaccgcga ctggtccctc tacgacacgg
     4681 cgcacgtcgt cttccgcccg gcgcggatga gcccggagcg gctcgccgag ggttacacgt
     4741 ggtgctacga ggccctcttc tccgccgggt cgatctggcg gcggaggccg ctcgacccgc
     4801 gagccgtcgc cccgtacctc gccatgtcgc tcctctacaa gcgcgcgaac ccgctctgga
     4861 ccttcctcat ccgccgccgc ctcgtcgcgg cggcctggcg gccgctcgtc gaagcgacgc
     4921 gccggcgtca cctcgtgttc cggcgccgcc tcgcgatgcg ggaggtctct gcggccccgg
     4981 cgtcgcggca gggggagacg ctcgctctct tcccgggagc ctgaggagcc gttacgccgc
     5041 cggcgggcgc aggcacccgg cgggcgcgac gaagccggac accttctcgg cgacgttccc
     5101 catggagacc cggcaggcgt gagagacgag gagggcgagg aggaggagcg cgtaggtgac
     5161 gtcgagccgc cgctcggacg tcgggggcag gtcgaaatcg agagcctggc ccgtcgccgc
     5221 gtcgaaccag atcgtgtcgt cgcgacccca gagccggaag cagacctctc ggaacggcac
     5281 gtggaggagc ttcgccgcgc ggcgggtcac ctcgtcgacg acgatcgccg caggtggaac
     5341 ggaggctggg acgatctcgg ccggacccgc ctctccctcc cgaaaggcga cccgatcgcc
     5401 ggccggtgcg cgaaacgctc cgaggttgct tgcgaggagc ggcgccgcgg ggatgagggc
     5461 gtcccccggg aggagccgga acgggaaccc gaggaggcgc gtggcggcct ccttcggatc
     5521 ggcgaggacc tcccggcccc tcagccagcg cgcgaggtgt ccgagggccg cggcctcgtc
     5581 gaggacgggg acgagccgct ggtgggcgat ctccgccacg cccccgaggt ggaggagcga
     5641 gccgcaggcc gggcagttgg cgaacacacc ctcctcgtcc gcgtcgaagc gaatgccgca
     5701 ccgcgggcag acgtgtcctt ccgccgtcac gtgggcggga tgttcaggcc aggaatgggg
     5761 aatcggcgaa gcgtcgccgg acgccggcgc ggaccgcctc ggagacgcgc tacgcgacct
     5821 cggcgcccct ctcggcgagg agcgcccgga ggatcgagcg gtggcaccgc gcctcgtcgg
     5881 cgcagtagca gccgaccgcg aggctcgtcc ggtgcgagag ggcggcgagg aggtccagga
     5941 gacgtgcggc gtgcggcgcg cccatctccg cgcggtactt ccgctcgaac gccttccagg
     6001 ccctctcgtc ggcggccgct tgcgccgcct tcacgagctc ctcgccgggg gcgaggtcgg
     6061 ggagccagac gtcgtagaag tcgcgcgagg cgaactccgc cttcgggacg ccgcgcggcg
     6121 gacgacgcac cgtcccgagc cgaaggccct cacccttgcc gcgcggggag ccgagccgga
     6181 cgacccggat cgccatggcc tcctccttca gcggtcgaac gactcggccc gcgcggcgga
     6241 cgagagcggg acgaggtagc gcccgggatc ggcggcgatc tttcggtgcg ccgcctcgag
     6301 ggtcgactcg cccgcgtagc tgacggccga gcggaggtgc ccggcgatcc gggcgaggac
     6361 gtcgacgacg ctcccgacgt acggcacgcg gacggactgg ccttcggccg gggtcgcgag
     6421 ggcgtcggcg acgccggcgt ccgtcgagcc gtcgacgacg gcctcggggg aggtcatgcc
     6481 ccggtagagc ttcgccttct gccgcgtcgc cgggtcgtcg acgacgatgc cgggggattc
     6541 gtcggtcccg gagaggagcg agccgagcat gacggtcgag gcgccgcagg cgagggcgag
     6601 gaaggcgtcc ttgtcattcc ggattccgcc gtccgcgatg atcgggacct tcccctccgt
     6661 cccgaggaac gcctcgcgga tcgcctggag ctgcgggacg ccggcgcccg tctcgaggcg
     6721 cgtacggcag ccgcgccccg gcccgacgcc gaccttcacc gcggcggcgc cgaggtcgga
     6781 gaggaagcgc gccccctcgg ccgtggcgac gttcccgacg acgagcggaa tccccgggaa
     6841 ccggctccgg aaggcgggga cggccttcgc gaggatctcg gaatgggcgt gggcgacgtc
     6901 catcaggacg acgtcgacct ccgcctcggc gagggccgcg gcgcgctcga ggtagtcccc
     6961 cgcggcgccg atcgcggcgc cgacgcggag gcgaccgcgc tcgtccttca ccgaccaggg
     7021 cttcttcttg aagaggtgga ggtcgcgcat cgtgacgagg ccgcggatcc tcccggcgtc
     7081 gtcgacgagg gggagcttct cgacgcgccg ctcccacatc gccttctcgg cctcctcgag
     7141 ggagacggac ggcggccggg tcacgagccg cgcgacaggc gtcatcagct cctccacccg
     7201 cctctcgttc gcgtccgccc cgtgcgggag gtcgcggtgg gagaggatgc cggcgagaag
     7261 gccggagccg gccgtctcct cgatgaggat gccgctcgag cggtgcttct cgatgagccg
     7321 gcgcacctcg gcgatcgtcg cggtccgagg gaggacgagc ggcttctcga tcacgtagga
     7381 atggcgcgtc ttgacgtagc ggacgcgcgc cgcttgcgcc tcgatcgggg agctccggtg
     7441 gaggaagccg aacgctccct cgagggcgag ggcgcgcgcc atctcctcgc cgacgacggt
     7501 gtccatgttc gccccgacga tcggcagccc gaggtcgagc ccgcggacga gcggcatcga
     7561 caggtccacg gcgcgacggg aggcgacggg gctccgctgc gggcgaaaga ggaagtcgtc
     7621 gaaggtgcgg ccgaagaaga agggctccgt ctcgcgcatc gccttcattc ttccatctcc
     7681 gtgccggatc ggatacgcct cggggtctgc gaggcgggcc gctagaatga gccgtcgcgg
     7741 acgccggcag gctctccctg ccgccgcgac cgacgaggag agggttctgg cagaagctgc
     7801 gcccccggtg ggtccggtgc ggagacgggg cctgggtgcg gtccccgcct gcatcgtgct
     7861 ttgcggagcg gtcttcatcg cccggaacgc cccggcgctc gacgtcgcgc ggcccctctc
     7921 ccaggcccag gtcgacgtct ggaggacgga gaacggcctc ccgcaggaca cggtcacctc
     7981 gatcctccgg acgcgcgaag gccacctctg gttcggcacg tacgacgggc tcgtccgttt
     8041 cgacggcgcc cggttcaccg tcttcgacgg gaagacgacg ccgctcctcg cgacgggctc
     8101 cgccttcgcc ctcatggagg atcggcacgg agcgatctgg gtcgggcgga gcgagaacgt
     8161 cctgaagtac gagaacggcg ccttcacgca ggtcctcggg gccgacgagc tggggcaggg
     8221 gaccgtctgg gctcttgccg aggccccgga cggcaccgtc tgggccggag caggccgcgg
     8281 cctcgtccgg tggacggccg gcaaggcgac gctcctgacg aaggagaacg ggctcccacc
     8341 cgcgcggctc cggacgctct gcgtcgacac ggacggggcg ctctgggtcg gcacgagcgg
     8401 agccggcctc gcgcgcctga aggacggccg cgtgacgcgc tggacggcga aggacggctt
     8461 cccgagcgac cagatcgtgg gcgttctccc cgatccgtcg ggcgggatct gggtcgcgac
     8521 ggccggagcg ggcctcgtcc ggatcgtcgg cgacagcagg cgcgtctgga cgacggccga
     8581 cggccttccg accgaccagc tcacggcgct cgcgctcgat gcggccggct cgctctggat
     8641 cgggacgtgg gggagcggca tctgccgctt ccaccgcggg accttctccg tcctcgcggc
     8701 gccgcccctc tcgaacgaca agatctggtg gctcctcccg gacgaggagg gcttcgtctg
     8761 ggtcgggacc tgggtcggcg gcctgaaccg gctccgagac cggagctttc cgtccttcgg
     8821 cgtgcccgag gggctctcga acgacaacgt ccgctcggtt ctccacgcgc gtgacgggag
     8881 cgtctggctc gccaccgccg gcggcgggct caaccgtctc cgggacggac ggctcgacgt
     8941 gatccggaag gccgacggcc ttccgagcga cgaggtctcg gcgctttgcg aggaccgtac
     9001 cggggtgctc tgggtcggca cctacacctc gggcctggtg aagctgcgag gcggacggat
     9061 cgaggcgttc gggaaggacg acggcctgcc cggagacgac gtacgcgtca tcttcgagga
     9121 ccgggagggg acgctctggg tggcgacgac gacgggcgtc gccacgtcac gcgacggccg
     9181 ccggttcgag cgggtcgcga cgccggagtg ggtctctctg aacgccgtcg tctgcatgct
     9241 cgaggaccgg cagggggcgc tctggttcgg cacctccggc gacggcctcg tccggcggga
     9301 tgacaccggc ttccgcgtcc tgacgacgag cgacggcctc gtgagcgacc ggatcgccgc
     9361 gcttcacgag gacgacgagg ggaccctctg gatcggcagc gcctggagcg gcatcaatcg
     9421 cctgaaggac ggccggatca cggcgattcg tcccgagaac ggcctcgcgg aagggcgggc
     9481 gcagaccctc atcccggacc gcttcggcgg gctctggctg accggcaaca agggcttcca
     9541 gaggctcctg aagaaggagc tgaacgaggt cgcggacggc cttcggagag tcgtccggcc
     9601 gctctccttc ggcctggcgg acgacctccg gagcgcttcg ttcgcgagcg gtcagcagcc
     9661 cgccgggtcg gtcgactccg acggccggat ctggctcccg tcctatcgcg gcatcgtcgt
     9721 cgtcgacccc gcccggatcc cgccgcctcc gccggccccc ggcgcgcgcg tcgaggaagt
     9781 cgtggtcgac gggagcctcc gtgccgcgag gcgggagctg aagatcgagc cggcgagaag
     9841 gctcctcgag gtccgctatg ccgcgacgac gctccggcct cccgagctcg tcgggttccg
     9901 cttccggctc gacgggttcg acgacgactg gcacgaggtg gggacgcggc gagccgccta
     9961 ctacaccgcg ctgccgcccg ggcggtaccg gttccgggtc cagagccggg ttggagacgg
    10021 cgcctgggga caggaggcgg agcccctcgc gctcgttctc gagccggcgt tccaccagac
    10081 gtcctggttc cggctcctcg ccgcgctcct cgtcctcgga ggggccggcc tcctcctggg
    10141 cagccggacc gcgcggctga agcggcggca cgacgagctc gagaggctcg tgggcgagag
    10201 gacggatgcg ctgcgcgaag ccaacgagcg gctctcggag ctttccttct ccgacgcgct
    10261 gacgggcatc ccgaaccgcc gccggctcga cgaggcgctg aacgcggagt ggaagcgggc
    10321 cgtccgcttc ggccacccgc tctccctcgt cctcgcggac atcgacttct tcaagcggta
    10381 caacgacgcg ctcggccacc cggccgggga ccgttgcctc gtggaggtcg cgcgcgtcct
    10441 gcaggcgaat gcccgccggg cgggcgacgt cgcggcccgc ttcggcggcg aggagttcgc
    10501 gctgctgctc ccggcgacca gcggaacgga cgccgcggcc gtggcggagc ggacccgggc
    10561 cgccctcgag gcgctcgcgc tgccccaccc ggagggggcc gccccggtcg tgacggcgag
    10621 cttcggcgtg gcgaccttcg tgccggaatc cgacggggac ccggaccgcc tcctcgcgac
    10681 ggccgacgcg gccctctatc gggcgaagag agaggggcgg aatcgcgtcg tcagcgccac
    10741 tccgcgcggc gggcgccctc cggaagccgg gcggtcggga gagcacccgc tcccgaaggc
    10801 gctctgaagc gcgtttcctc cccggccgag ggggtgggaa actccgtcct tccgccatga
    10861 cgatccccga gaagccctcc gtcacccccc tcgccgaccg cgtcccgccc cgcgggacga
    10921 ccgacggcga cgagatcctc ggcctcttcc tcgactgggt cgccgacacc gggctgacgc
    10981 tctacccggc ccaggaggag gcgctcctcg agatcatggc gggcaagcac gtcatcctcg
    11041 ggacgccgac cgggtcgggg aagtcgctcg tcgccctcgg gctccacttc aaggcgctct
    11101 gcgaggggcg cgtctcgtac tacacgagcc cgatcaaggc gctcgcgagc gagaagttct
    11161 tcgcgctctg caacgagctg ggagcggaga acgtcgggat gcggacgggc gacgcgagca
    11221 tcaacgccga cggcctcgtc gtctgctgca cggcggaggt cctctcgaac atggcgctcc
    11281 ggctgggcga ggagctcgac gcgccctacg tcgtcatgga cgagttccac tactacgccg
    11341 acaaggagag gggcgtggcg tggcaggtgc cgctcctcgt cctgccgggg acgcagttcc
    11401 tcctcatgtc ggcgacgctc ggcgacacct ccgcgatcgc cgagcgcctg acgcacgaca
    11461 cggggcgcga ggtcgcgttc gtgacgtccg aggagcgccc cgtcccgctc gacttcgcgt
    11521 acgccgagac gcctctgcac cagacgatcg agaagctcct cgaagcgggg aagagcccgg
    11581 tctacgtcgt caacttcacg cagcgggagt gcgccgagct ggcccaggcg ctgacgagcg
    11641 tgaggatcgg gacgaaggag gagcgcgagg cgatccgcga ggtcgtcgcc ggccggcggc
    11701 tcgtcacccc ctacggcaag gagttccggc gctttctctc gttcggcgtc ggcgtccacc
    11761 acgccgggct cctgccgcgc taccgcctcc tcgtcgagca gctcgcccag agagggctcc
    11821 tccacgtcat ctgcggcacc gacacgctcg gcgtcggcgt gaacatcccg atccggaccg
    11881 tcctcttcac gaagctcgcg aagttcgacg gggcgaaggt cggcctcctc accgtccgcg
    11941 agttcaagca gatcgccgga cgcgccggcc ggcgcggctt cgacacgcag gggagcgtcg
    12001 tcgcgcaggc gcccgagcac atcgtcgaga agaagcaggc gacgaagaag tccgacgcct
    12061 ccgggaagaa ggtcacgaag acgttccggg ggccggcgaa gggggagatc tcctggaccg
    12121 aggagacctt ccagcgcctc atcacccggc cgcccgagac gctgaagtcg cgcttccacc
    12181 tcacgcacgg gatggtcctc gacctgatcc agcgggacgc cgacacgaac cgcgacgccc
    12241 cgcctccgga gaggaacttc tcggcgctcc gcgacctcgt cgcccgctgt cacgaggagg
    12301 acgcgacgaa ggcgcggctg ctcaagcacg cggcgctcct cgtccgttcg ctctaccggg
    12361 cggggatcgt cgggatgtcc gtcgaggaga ccggcttccg gcgcgtcgtc gtcgccgacg
    12421 acctccagtg ggacttctcc ctccaccagg cgctctcgat ctacgccgtc acggcgctcg
    12481 agcggctcga ccccgcctcg ccgacctacg ccctcgacgt cgtcaccgtc gtcgagtcga
    12541 tcctcgagaa ccccgacatc gtcctccgga agcagaccga ccgggcgaag ggaaggctcg
    12601 tcgaggagct gaaggccgag ggggtgccgt acgaggagcg gatggcgaag ctggaggagg
    12661 tcacgtaccc gcagcctctc gccgacttcc tcttcggcgc cttcgaggcg ttccgcgagg
    12721 cgcacccgtg ggccggcggg accgagccga agccgaagtc gatcgggcgc gagatgttcg
    12781 agaccttcgc gggattctcg gactacgtga agagctacgg cctcgagcgg agcgaaggcg
    12841 tcctcctgcg ctacctctcg cagctctacc ggacgctcga ccagaccgtc cccgagccgg
    12901 cgaagacgga cgagctctgg gacgtcctcg gcttcttccg cgcgctcgtc ggtgagacgg
    12961 acgcgagcct cctcgaggag tgggagagcc tccgtcaccc cgaggagctc ctcgaggcga
    13021 aggccgagcc ggagaagccg aagcaggccg cgtggctccg cgagctgctg tccgacccgc
    13081 gcgtcttcgc ggcgcgcgtc cgctccgaga tgcacctcct cgttcgcgcc ctctcgcggc
    13141 aggactggga ggacgccgcc gagcgggtct ggcagccggc ggagaacgcc gaaggcgcag
    13201 cggaggcggc ctggacgccc gagcgcttcg aggcggccct cgcgccgttc ttcgaggagc
    13261 acgccgagct cctcgcgggc cccgagtcgc gcctccacca gtggacgagc ctgaggccgg
    13321 ccgggccgcg gcagtggacc gtgacgcaga cgctcctcga cccggaggaa gagaacgcct
    13381 gggccgtctt cgccgggatc gacctgggcg acgagacggc ccccgacggg ccgatcctcc
    13441 ggctcacgcg gatcgggacg taggcgcccg ggcccgacca atgacgccgg gcgctctcgg
    13501 acggatggcc ggttacggac gcgtctcggc gcgctcgagg aaatcctgga cgacccggag
    13561 aaagctctcc ggctcctcca ggtgaggcat gtgcgcgctc tcctcgaata cgactcgttc
    13621 ggacccgtga atcccgcgct gcagcgcctc cacgacggcg ggggtcgcct cgtcgaaacg
    13681 gcccgagagg acgagcgtgg ggagccggat ctcgccgagg cgaggggtga cgtcccactc
    13741 cttcaggacg cccgtggcgt ggaactcgct cggtccgaac atcacgttgt agacctcggg
    13801 gtcctcggcg aggtttgcga acgtccgctc gaggcactcc ggccacgggt cgagtcggca
    13861 gacgtgccgc cggtagaagg tcaggacggc cccctggtac tccgtggagc cggtcgtgtc
    13921 gtccgcctcg tgccgcgaga gggtcgcccg gacctccgga gggagctcgt cgcggaggcg
    13981 gttcgtctct gagatccaga gcggaacgct cgcgagcgag ttcgcgagga cgaggctcac
    14041 gagacccggg tgcttcgcga gcgccgtctc gagcgccagc attccgcccc acgactgccc
    14101 gaggaggtgg acgcgaggga ggtcgagagc cgcccggacc gcagccacct cgtcgacgaa
    14161 gaggccgacg tcccagagcg acgggtcgtg cgggtggtcg gagcggccgc agccgagctg
    14221 gtcgtagaag acgacccggc ggcccgaggc ggcgagggcg gagaggggct cgaggtagtc
    14281 gtggcccgtc ccgggtccgc cgtggaggac gaggacgggg tcccgtccgg gcgcctctcc
    14341 ctccccgacg acgcggtacc acgtgcggaa cccccggaac gagacgtgcc cctcgccggt
    14401 cgcccgctca cccatctccc tctcctccct cggcggcgtc cccgacgggt cccgcggcac
    14461 cggaccttgg cctcagcggt cgacgcgacc gaagatgccg ccgccggcgt cggcgcacat
    14521 gtccacgacg agccgctcgg cgggacggcc ggccggtacg gcgaacgtca cgcccgcggc
    14581 catctccgga tagctcggca gggagagcgc ccagacgttc ccgctgtagt gcgtcaggac
    14641 gccttcgatc ttccaggggc ccatcgtcat gacgaggcct ccccccttct ccgtcaccgt
    14701 gaaccggccg tacgccgggt tctcgtagac gccgacgtag cgcgcgagcg ggagcgacgg
    14761 cagcggggcg agtagggcgg gcttcggcgc cgcggcgatg cggaacgatg ggaggggctc
    14821 ctcctcggcg aggagctgcg cccggcgcgc gagcgtcggc gcgggcggcg tgaagaggag
    14881 gtcgaagagc ttcgacatca tccgctcggg gacctggttg tccgacgagt tcgtcaggac
    14941 gacgagggcc gcgttcgcgg cggggtagag ggcgacgatg gagtgctgac cgctcgtgcc
    15001 gccgttgtgc cagacgatgc ggcccgcccg gcgcgactcc gagatccagc ccatcgcgta
    15061 cgagccgctg gagccgtcgc gggcgaaggc gagcgtcctc ggggcgtgga gcaggtcgac
    15121 cgtcccggga gcgaggtgcg gctcgccctc gaacgtcccg cgggcgaggt ggaggcgcgc
    15181 ccaccggctc atgtcgagga cggtggagcg gatgctgccg gcggggccgt acgtgtcgat
    15241 ccagccccgg aacggccagt ccggcgggat cggtcgaagg ccgccgtccg cctggcgaag
    15301 gtgcccggat gcgacgtcgc cggccatccc gacgacctcg gggtcgacgg tcgtctcccg
    15361 catgccgaga ggcccgagga cgcgaaggtg gacggcgtcc tcccagcgct gaccggtgac
    15421 cttctcgagg acctcggcgg cgacgagcca gagggtgttc acgtacccga acgtgttccg
    15481 gaagctcgac accggctcga cgtggacgag cgcctgctgg atctcgtcgc gcccgaagcc
    15541 gatgaaggcc atccagtcga gcgagtaggg cggcatcccg ctccgctggc tcacgaggtc
    15601 ggcgatccgc atctcgcgcg tcacccaggg gtcgtgcatc cggaagcgcg ggaggacgtc
    15661 gacgacgcgc gtgtcccacg agatcagccc ctcctccacg gccatgccga ggagcgccgc
    15721 ggtgaacgcc ttggacgtcg acccgatctc gaagagggtc gacgtcgtga ccggggcggc
    15781 ctcgccggcc ttccgctcgc cgagggcctt cgagaagacg acggcgtcgt ctttcacgac
    15841 cgcgtacgcc atcccgggaa tcccttccgc gcccctcgcc tcgagggcga acgcttcgag
    15901 ggcggaaagg acgtccgtca tcgagcggcc ggaggggaca ggggtctccg ccaggagggg
    15961 cgggaagacg aggagagcgg cgccgagggc cagcgaaacg agagggtggc gacgggtccg
    16021 atcgcgcatg gaacctccca gcatcgactc tggcccgccg gggcggctca gttccggatc
    16081 gaccggaccc ctcgtcgccc gagtcgcgaa acgagtatcg cccccgcggc gcccgtcggc
    16141 cacacgaaag gccgaagcgg tctcctccag cgcggtcgag cgccgccgat atcatcgagg
    16201 ccgcatgacg ctcgcctgcg acgtcgccct cgccgcccgg atcgaacggg ccgaggccct
    16261 cctcctcgcc gacgccgtcg cccgggtcgc cctccgcgtc ggcgagcggg gcgacgtcct
    16321 ctcgcgcccc ctggccggag ggatcgcggc gttcgcggag ccgggctcgc cgctgaacaa
    16381 ggtcgccggc ctcgggttcg acggcgcgct ccccgagacg gagctcgaag aggtggagaa
    16441 gggcttcgcg cgccgggagt gcccggttcg cgtggagctc tcgagcctcg cggacccggc
    16501 gctcggcgcc ctcctcacgc ggcgcggcta cgttctcgag ggtttcgagg acgtcctcgg
    16561 cctcgcgctc ccgacgcggg gctttccgcc gattcccgag gacgtgaagg tccgtccgtc
    16621 cggacccgaa gagctcgagc tctggttgga cgtcaccgtc gccggcttcc tctcgccgga
    16681 cacagagggg gttccctcga acgagtcgtt cgaggaggcg gtcctccggc gggtcatccg
    16741 cgacttcgca caggccgaag ggatgacgcg gtacctcgcg gagcggggcg gcgctcccgc
    16801 cggcgggggg agcgtgcgca tcgccgacgg ccttgcccag ctctgcggcg ccgccacgct
    16861 ccccgcgcac cggcgccgtg gcatccagac ggcccttctc tcggagcgac tcgcggccgc
    16921 gggccgcgcg gggtgcgacc tcgcggtcgt cacgacgcag cccggctcga agtcgcagga
    16981 gaacgcccgg cggcacggct tcgcgctcct ctacacgcgc gcggttctcg tccgcacggc
    17041 ctgaagggcg aggccgccgg ccggactcga gcgagatcct tcccggatcg caagtctgac
    17101 tttcgattgt catgacaatc gaaagctgat gtttcatcgc atctgcgccc tccccgccca
    17161 tcccctcgag tcgttcttcc tccgggaccc gcgccaggcg ggaaagagca ctctcccgaa
    17221 ggcgctttac tccgacgcgg cctggttcga gctcgtccga gcgctggacc acggtctcct
    17281 gtcgaggcgt ccggggcgac tagaatgccg ccgtgccagc cgttccgggt ctccggcggg
    17341 gtctctcggc gctctacggc gagcggctga agggggtcta cgtcttcggc tctcgagcgc
    17401 gcaccgacgc ggccatggac tcggacctcg atgtcctcgt cgtactcgat cggatcgaca
    17461 gctatgccga ggagctcgag agaacgagcg cgctgaccgc ggggctctcc ttggcgctgg
    17521 ggttcacggt cagccgcgtc ttcgccgccg aggcggactg ggagacggcg aagtcacctt
    17581 tcctggccgg agtccgggct gacgcggtgt ctgcatgaac gaagcgtctg ccgccttcct
    17641 ggcaaaggcg gaacgagcgc tcgacgccgc cgagaccctc ctgcgcgaag ggcacgcgga
    17701 gttcgcctcg gggcgcgcct actacgcgat gttctacgcg gccgaagcca tgctggcaga
    17761 ggccggggag gccttccgga agcactccgg ggtccacgca gctttcggtc gccgctttgc
    17821 gcagggcggc gagctggacc cgaaattcca ccgttggctg ctcgatgccc acgacgcccg
    17881 actggcggat gactacggct tcgagtcggc cctgacgaac gaggatgtgc ggacgatgct
    17941 cgaccgggca gcggagttcc tcggggcgac gagatcctgg ctggccgctt ggccttcgtg
    18001 aagggactcg cgcgggggag gcggcctgga gaacctccac gaccgcctcg tctgtgctgc
    18061 ctcagagctc gagcctgcgc agccggagcg agttcacgat gaccgagacc gagctgaggc
    18121 tcatggcgac tccggcgatc atcggcgaga ggagccagcc gaagaccggg taaaggagcc
    18181 cggcggcgag agggatgccg aggaggttgt agccgaacgc ccaggcgagg ttctgcctca
    18241 cgttccgcat cgtggcgcgg ccgaggcgcc gcgcgcggac gaggccggcg aggtcgccgt
    18301 ggaggagcgt cacggaggcg ctctcga
//
LOCUS       DAOTEZ010000023        18118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2078_length_18118_cov_6.524608, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000023 DAOTEZ010000000
VERSION     DAOTEZ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 18118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18118
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2078_length_18118_cov_6.524608"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="P5164_02160"
     CDS             <1..896
                     /locus_tag="P5164_02160"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRY42726.1"
                     /translation="GEIALLARVARPDAALVTLIAPEHTEGIGTIEDVAEEEGDLFAA
                     LGADGCAIGNGDDPRVAAQIARAPGERRILYGFGEGCHLRAVARESRGLGGSLLHVAL
                     AGAEAFAYEVPLAGEAGAYAALAALAAVRGLLGVDAPPEALREALLRVASVRDGRFAP
                     ETLADGTVLLDDTYNSNTGSALSSLRTARELADALGRRLVLVLGEMRELGPLAAAEHD
                     VVAGAAVAARPAALVAVNGEAARFARAAEAAGVPAAFFETGEEAAPRVVETVRPGDVV
                     LVKASRGVRLETVAAALRAAR"
     gene            919..1434
                     /gene="purE"
                     /locus_tag="P5164_02165"
     CDS             919..1434
                     /gene="purE"
                     /locus_tag="P5164_02165"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRY42727.1"
                     /translation="MSTPLVGIVMGSASDHETMAKAEEVLRDFGVPYETSVVSAHRMP
                     EEMFDYAKGAAARGLKVIIAGAGGAAHLPGMVSALTTLPVLGVPVQSRALSGLDSLLS
                     IVQMPGGVPTATFAIGPAGATNAGLFAVKVLALSDPALAAKLEAHRESLREKARAGNA
                     EVRSPRGGTAP"
     gene            1431..2624
                     /locus_tag="P5164_02170"
     CDS             1431..2624
                     /locus_tag="P5164_02170"
                     /inference="COORDINATES: protein motif:HMM:NF014298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRY42728.1"
                     /translation="MTTVGILGGGQLGAMLASSLQDLGADVHVFDPDPTAPALRRAAR
                     AVSAPWRDTARLQELFDACDVVTYEFENVETEGLARVSGLEKLAPSLDVLRTTQDRAL
                     EKEFFRAHGLPHVAFAAGRDADDLRAAVRSFGLPAILKTGRGGYDGKGQWKLASEDEL
                     EALLASPARDALEAGGWVLEEPIEIVLEASAIVAREKNGDEVVFPLFENVHRDHILDV
                     TLVPARLPVDLEAEAKSVALSAARALGVTGLLTTELFVGRSARHRSTDVRIFTNELAP
                     RPHNSGHVTRKACTYSQFEALARVLLGVPLVPPRLLSGGAFCMANLLGEVWQAQGSEG
                     SLDLSAWEGNPDLLEVVLYGKSGVATKRKMGHLVARGAGAEEAIAAARACRKALSRRG
                     PQGSL"
     gene            2637..3479
                     /gene="ccsA"
                     /locus_tag="P5164_02175"
     CDS             2637..3479
                     /gene="ccsA"
                     /locus_tag="P5164_02175"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRY42729.1"
                     /translation="MSGTGLSNLLFGLLALVCYGGATLLYVTWLRHPKVSAGRLATLL
                     AAAGAVFNFVFLYVRSLALKTVPYGDLLGSMALFGLFLAAMSLILEARHHDRSLGAFL
                     MPVSLFFLLHSFLLPGGSLPKQEMRGSIFAFHVTLNMLSYSAFAVACALSLLYLVLGR
                     RLRSKRHVLNGPVSRLPSLSYLERAARTSIGVGVVSLAAGLTMGFLWAYKVWHVEHPG
                     WLLDAKVWGSVVTFAFYLWVFVRAHRGAAPGSTAKLSIAGFVLVLLSYTAVNLFFSKV
                     HSFA"
     gene            3479..4753
                     /gene="hemA"
                     /locus_tag="P5164_02180"
     CDS             3479..4753
                     /gene="hemA"
                     /locus_tag="P5164_02180"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HRY42730.1"
                     /translation="MPPEIVFVGWSHQGADLDLRARLAFTPEKAREALEGLFRERILT
                     EGAVVSTCNRAEVYGVSEVPDSIDALAEFFSRFHGVEASVLREKAFSGQGEATVRHLF
                     RVASGLDSMVLGEAQILGQIREAHRRAAEAGTARAVTSRLFQSALECGKRVRTETSLG
                     ARPVSVPGIALSLVGRIFESVSDCKVLLLGAGETVELTARLLADDGVKQITFTNRSPE
                     KAKALAGCFHGWTVPWEERAKAFAEVDIVLSATGATEPVVTFDMLKPVLGRGRRRGPL
                     LILDVAVPRDVDPRVEEFSDVYRYDMDALGELACDNAKDRMADVPQAERIVEESTERF
                     LGWLAGLSHVDTLKTLRGRLEKARVDELERYAGKLAHLSAEDRAVVERMTETLIHKIL
                     HNPTIGLKEGDASERLTRAAVVRALFRLDGGE"
     gene            4884..5837
                     /gene="hemC"
                     /locus_tag="P5164_02185"
     CDS             4884..5837
                     /gene="hemC"
                     /locus_tag="P5164_02185"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018104367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HRY42731.1"
                     /translation="MSHGRPLRMGTRASLLARTQSGHVAAALTKATGRAVEEVLVRTE
                     GDQLSTEGRIPAAGDSVGVFVRALDDALRRNEIDFAVHSLKDVPTEYAEDLLLAAVPV
                     RADVRDALVVASRHDAKDVRSLPQGARVATASPRRVAQLLRLRPDLETVTMAGNVDTR
                     LRRLEEGRADALILACAGLDRLSLGERITQRIPLDELLAAPAQGALGISCRRDDADAA
                     AAISHLDDPATNAAAAAERSLLNALRAGCRAPVAALATPGGGGTLALRARVLSLDGSA
                     MIEDEASGTVEDPLALGRLLAERLLARGAEQLVTEARAAGR"
     gene            5834..6535
                     /locus_tag="P5164_02190"
     CDS             5834..6535
                     /locus_tag="P5164_02190"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HRY42732.1"
                     /translation="MRRVLLLRPTGGPVVGADLLVTHRVVPVPAALAETAAYTAEGAI
                     LVVSSLNAVDMLSDAPEGNLLVRPFRLVVSAGAGTSRALEAAGVAEVVTPDPPGAVGI
                     LAELARRGESGRLLWPRGSDADVAPLSELASRGFEVTAPIVYVKEPLTALDARTLSAF
                     RAGEYDALAIGSLAALDVFLSALGASAARELPPVRFGVLGPETARLLVARGFPEPSVP
                     AKPLLTDLIALLKEA"
     gene            6539..7516
                     /gene="hemB"
                     /locus_tag="P5164_02195"
     CDS             6539..7516
                     /gene="hemB"
                     /locus_tag="P5164_02195"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009647102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HRY42733.1"
                     /translation="MAFPLHRYRRLRLSESLRTMVRETTLDPADFIAPMFVVPGEGIR
                     QPIPSMPGVDRVSPDLAAKDAAELAARGVLSVILFGIPDHKDAVGSPSADLSGPVCRA
                     VEAIKRASPATVVMTDVCLCEYTDHGHCGVIVDQAVDNDATLPILAAEALAHARAGAD
                     VVAPSDMMDGRVRAIRDVLDASGFRNVPILSYAAKYASGFYGPFREAAESTPAFGDRR
                     AYQMDPANVREAVKEILSDVEEGADMVMVKPALSYLDVVRAAKEVTNVPLATYNVSGE
                     YAMVKAAAERGWIDGKRVTLEILTSMKRAGADLILTYHAKEAAEWLQGR"
     gene            7498..8895
                     /gene="hemL"
                     /locus_tag="P5164_02200"
     CDS             7498..8895
                     /gene="hemL"
                     /locus_tag="P5164_02200"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019533467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HRY42734.1"
                     /translation="MAARKMRRKAAAPPPAPKKKAPKRPVAPRRRRVRRTVESDRLYA
                     RAKAKMPGGVSSPVRSFKSVGATPFFVKKAKGSILVDADGNRYVDYVMSYGPHLFGHM
                     PKAVASAIRKALPRGTSYGAPCRAEVELAERVTRLVPSMEKVRFVSSGTEAAMSAVRL
                     ARAITGRDLVLKFSGCYHGHADSFLVAAGSGLATLGIPGSPGVPAELAKATITATYND
                     LESVEALFRKYPGKIAVIAVEPVAANMGVVLPKPGFLEGLRKIADREGALLLFDEVIT
                     GFRLARGGAQELFGIKPDLTCLGKILGGGLPVGAYGGRADLMAHVAPDGPVYQAGTLS
                     GNPLAMAAGIAMLDSLTPAAYAKLERTAAKLEKGMRKVAKELGVEERLTLNRVGSILT
                     LFFNPGPIASFDDVKKSDGAAFNRWFHGLLKSGVFIAPAAFEAMFVSTAHTEKDLAKT
                     LAAHREALKEAFAEG"
     gene            complement(8959..11679)
                     /locus_tag="P5164_02205"
     CDS             complement(8959..11679)
                     /locus_tag="P5164_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRY42735.1"
                     /translation="MDRKCRRLLRLSPLVVAFCVVAFAGSARAECPQPSYGPGVVVAA
                     DTDLIVAGDFDGDGKANDVAVARTGTTAAIRFFKRDAAGSFVPLGTASAGVGNVWGLF
                     AGDLDGDRRTDLVASGSGPRSKVLLNRSLAGGPGGGGPTGGDPDDYFGFEDKGSPLAV
                     GESARFQVLAIGDATGDGKADLIVPYVMPSAATRLALYPGNGDGTFRPERTLDDVTDR
                     QYVNAAVGDLNGDGRPDAVAVLYPPELVVLLGDGAGAFAAPWRRSLPHASGTAAQGLA
                     LADFDRDGILDVAIGSYGSPTPASSIFFMKGKNDGSFEAAVASPAGTNPWNLAVGDLN
                     RDGVPDLVASNDSAEMKVAVLAGKRDGGFAPLLLLSTLGPTRFSPVTLGDLNDDRAPD
                     ILTNGTAFLNANACAPEGTARVVPIVLDVDTGSAHYVSELALTNRSSGPATATLAYTA
                     TLGSKAGSGSTTLTLGAGEQRRIDDVLGYLRSAGLPLPTAKADPQQGGTLKVAFVGPA
                     VDETNVAATARTTSATLAPLPVGRAGLAYGGVRASAASPASVTLFPLRNAAGVDRTNL
                     AVVNTSAEPMTIRITLFSATEPGRTVVFRQDETLPPYGWIQYGSAQLLEANGLTTAWA
                     KIDRTSSTGGLTAYAVINDAVTNDGSFVPPVASGSASATYLTVPVLVETSGFQSELFV
                     ANTASVDAVATIRYVESATPSAGPGGTFRLDLPAGQAARFPDALDWLRQKAGGVGPRY
                     SMSSYVGSLRIEVEGVPAASILAGARVAARTPSGKGQFGLYLPGVLGGEEAKSEAFLY
                     GLRSDEENRSNVAAVHTGPTGSGAIEVAFQCFDGEVGGAPAGERVYRTLEPGGWTQIG
                     NFLRTSNVRNGWVRVERTKGTAPWVAYGVVNDGAAAAERTGDGAFVPMVK"
     gene            11937..12491
                     /locus_tag="P5164_02210"
     CDS             11937..12491
                     /locus_tag="P5164_02210"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY42736.1"
                     /translation="MTRRPWTPPETIETPRLRLRRPRADDAEAVFAYARDPAVTRLMD
                     WPTHVSVETSRRFLAFCETGWETGTEFTWLLTLPPSDRAVGAIACRPKERGVELGYVL
                     HRDVHGRGLATEASRALVGWLTSMPDVVRIEATCDVENEASARVLEKAGLARERLLPA
                     YGARPNLGPDPRDAYLFAWTRPAD"
     gene            12527..13030
                     /locus_tag="P5164_02215"
     CDS             12527..13030
                     /locus_tag="P5164_02215"
                     /inference="COORDINATES: protein motif:HMM:NF024076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HRY42737.1"
                     /translation="MALSVFDDPNQPPEPAVLRRRLGPVATAWSALVDGVAERCGPIE
                     ELWKYGGARFGWSLRLLRKGRVLLYLTPLEGRLLAGLVLGGKAVAAAREAGLPPALLA
                     QVDAAPQYAEGRGLRVEVSGPDDVEGLLLLVGLKADPSVPPPVTAQSPSRAGRPRGGG
                     GIRRGGR"
     gene            complement(12960..13553)
                     /locus_tag="P5164_02220"
     CDS             complement(12960..13553)
                     /locus_tag="P5164_02220"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HRY42738.1"
                     /translation="MPEGARCPWAGSDPLYVSYHDEEWGVPSRDERHLFEMLVLEGAQ
                     AGLSWLTILRKRESYRRAFAGFDPEKVARFGPGDVKRLLGDAGIVRNRLKIESAIDSA
                     KAYLALQREAGSFSSWLWAHVGGRPVQGAFREMGEVPAKTPLAESISKELRIRGFRFV
                     GPTIVYAYLQAVGVVNDHLVGCPRHRAVARLGTGSGR"
     gene            13615..13737
                     /locus_tag="P5164_02225"
     CDS             13615..13737
                     /locus_tag="P5164_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42739.1"
                     /translation="MRPIVRDLAGEAILHGSLPGRLFRTALAALLAASAVLLAG"
     gene            complement(13754..14980)
                     /locus_tag="P5164_02230"
     CDS             complement(13754..14980)
                     /locus_tag="P5164_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016858998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRY42740.1"
                     /translation="MTAEADGRAPRAVPSGESLFARLWRLDPGVSFLNHGSFGACPAA
                     VLDRQAELRAELEREPVDFLVRRLPERLAEVRAVLGPFLGAEPDDLAFVPNATTGVNA
                     VLGSLDLGPGDELLTTTHAYPGCRNALAHVCGRTGARLVVADVPFPLGGEEDVVGPVL
                     SAVSPRTRLALLDHVTSPTALVFPIARLVAALRERGVETLVDGAHAPGMVPIDLDRLG
                     AGWYTGNAHKWLCAPKGAAFLHVRRDLRTRARPAVTSLGYSEEASPARFRAEFDWTGT
                     CDPTPWLTIPAALDALGALLPGGWPEVMTRNRALALEARETVEKVLHVPPAAPAEMVG
                     SMASVPLPKAAPGSTATRLGPQGLGGWLRERGVEAWLYPWPSPGGMLVRVSAQLYNAR
                     AEYERLGTLLAEALDD"
     gene            complement(14977..17037)
                     /locus_tag="P5164_02235"
     CDS             complement(14977..17037)
                     /locus_tag="P5164_02235"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRY42741.1"
                     /translation="MPTNPRDARAVEPLVGVLTEIARTVTETLSLPDAFARVAGAARR
                     VLPFDAAGVVRIVDGPALLSWGLATPDGAEAGRRYGRDDVSPLLWPGAASPRRIDEAA
                     VALDATRQADRELLDAGMRSMLVATIVRGDVPIGDLWFASRAPRGFGPADEPTAQAIA
                     DILSATLEHERLAEEERRRIRRHRELDSLAPALARALDVREVFDQVSAIAREVLPHER
                     MTLGLLSEDRTENRMWAVSGDRDWVPDSYPIPESERAQMVADYQIVGDAEADLPADSS
                     RRAILLAAGLRSSLRVPIRLEGKTVGALSFHARETQSFREEDVDLALRVADQVALAFS
                     HQRLAEEARRAEEARQEAARLEVRVRALADELESRDGFGRVVGASPAWRSVLVQASKV
                     APTETTVLLTGESGTGKEVVARAVHRASARSSGPFVALNCAALPEQLLESELFGYEKG
                     AFTGAYASRAGRLEQAAGGTLFLDEAGETSPLVQAKLLRVLEAREFQRLGGAKVLKAE
                     VRIVAATNRDLHAAMARGDFREDLFYRLSVFEIHLPPLRERPEDVLLLTERFLEDLSR
                     NVGRPAPGVSREARELLLAYSWPGNVRELRNALERAVILSEGGLVQAEHLPIAVARSG
                     EARVASASAIELPPGGLDLDALEKTLVTQALEKARNNRSRAAKLLGLTRAQLYTRLER
                     YGLA"
     gene            17082..18020
                     /gene="meaB"
                     /locus_tag="P5164_02240"
                     /pseudo
     CDS             17082..18020
                     /gene="meaB"
                     /locus_tag="P5164_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016697223.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
BASE COUNT         2455 a         6581 c         6552 g         2530 t
ORIGIN      
        1 gcggggagat cgccctcctc gcccgggtcg cgaggcccga cgcggcgctc gtcacgctga
       61 tcgccccgga gcacacggag gggatcggga cgatcgagga cgtggccgag gaggaggggg
      121 acctcttcgc ggcgctcggg gcggacggct gcgcgatcgg aaacggcgac gacccgcgcg
      181 tcgcggcgca gatcgcccgc gcccccgggg agcgccggat cctctacggc ttcggtgagg
      241 ggtgccacct gcgcgccgtc gcgcgcgaga gccgcgggct cggggggagc ctcctccacg
      301 tcgccctcgc gggggcggag gcgttcgcgt acgaggtccc cctcgccgga gaggcgggcg
      361 cgtacgcagc gctcgccgcg ctcgcggcgg tgcgggggct cctcggagtc gatgcgccgc
      421 ctgaggcgct ccgcgaggcg ctcctccggg tcgcctcggt ccgggacggg cgcttcgcgc
      481 cggagacgct cgccgacggg accgtcctcc tcgacgacac ctacaactcg aacaccggct
      541 cggcgctctc ctcgctgagg acggcgcgcg agctggccga cgcgctcggg cgcaggctcg
      601 tcctcgtcct cggcgagatg cgcgagctcg gaccgctcgc tgccgccgag cacgacgtcg
      661 tcgcgggggc cgcggtggcg gcccggccgg cggcgctcgt cgccgtgaac ggcgaggcgg
      721 ctcgcttcgc gcgcgccgcg gaggccgccg gggtcccggc ggcgttcttc gagacggggg
      781 aggaggcggc accccgcgtc gtggagacgg tccgtcccgg ggacgtcgtc ctcgtgaagg
      841 cctcacgcgg cgtccggctc gagaccgtgg ccgcggccct ccgggccgcg cgctaatctg
      901 gggacggagg ccgaagagat gtccacgccg ctcgtcggga tcgtgatggg gagcgcctcg
      961 gaccacgaga cgatggcgaa ggcggaggag gtcctccgcg acttcggcgt gccgtacgag
     1021 acctcggtcg tctccgcgca ccggatgccc gaggagatgt tcgactacgc gaagggagcg
     1081 gcggcgcgcg ggctgaaggt gatcatcgcg ggagcggggg gcgcggcgca ccttcccggc
     1141 atggtctcgg cgctgacgac gctcccggtc ctcggtgtcc cggtccagag ccgcgccctc
     1201 tcgggcctcg acagcctcct ctcgatcgtc cagatgccgg ggggcgtccc gaccgcgacg
     1261 ttcgcgatcg gccccgccgg cgcgacgaac gccgggctct tcgccgtgaa ggtcctcgcg
     1321 ctctcggacc cggcgctcgc cgcgaagctc gaggcgcacc gggagtcgct ccgggagaag
     1381 gcgcgggcgg ggaacgcgga ggtccggagc ccgcgcggcg ggactgcgcc gtgacgacgg
     1441 tcggcatcct cggcggaggg cagctcggcg cgatgctcgc ctcgtcgctc caggatctcg
     1501 gcgccgacgt ccacgtcttc gaccccgacc cgacggcccc ggcgctccgc cgcgcggccc
     1561 gcgcggtgag cgctccctgg cgcgacaccg cccgcctgca ggagctcttc gacgcctgcg
     1621 acgtcgtcac gtacgagttc gagaacgtcg agaccgaagg gctcgcccgc gtctcggggc
     1681 tcgagaagct cgccccgtcg ctcgacgtcc tccggacgac gcaggaccgg gcgctcgaga
     1741 aggagttctt ccgagcccac ggcctgccgc atgtcgcgtt cgccgccgga cgggacgcgg
     1801 acgacctgcg tgcggcggtg cggtcgttcg ggctccccgc gatcctcaag accgggcgcg
     1861 gcggctacga cgggaagggg cagtggaagc ttgcgtcgga ggacgagctg gaggcgctcc
     1921 tcgcgtcgcc ggcgcgcgac gcgctggagg ccggagggtg ggtcctcgag gagccgatcg
     1981 agatcgtcct cgaggcgagc gccatcgtgg cgcgcgagaa gaacggggac gaggtcgtct
     2041 tccccctctt cgagaacgtc catcgcgacc acatcctcga cgtcacgctc gtgccggccc
     2101 gcctccccgt cgacctcgag gcggaggcga agtccgtcgc cctttcggcg gcccgggccc
     2161 tcggcgtcac cggcctcctg acgacggagc ttttcgtcgg gcggtcggcg cgccaccgct
     2221 ccaccgacgt ccggatcttc acgaacgagc tggcgccgcg cccccacaac tccgggcacg
     2281 tcacccgcaa ggcttgtacg tacagtcagt tcgaagctct cgcccgggtc ctcctcggtg
     2341 tccctctcgt cccgccgcgt ctcctctcgg gcggcgcctt ctgcatggcg aacctcctcg
     2401 gcgaggtctg gcaggctcag ggatcggaag ggagcctcga cctctcggcc tgggagggga
     2461 atcccgacct cctcgaggtc gtcctctacg gcaagagtgg cgttgccacg aagcggaaga
     2521 tggggcatct cgtcgcccgg ggggccgggg ccgaggaggc gatcgccgcc gcacgggcct
     2581 gccggaaggc gctttcgcga agaggaccgc aggggtcctt ataatcagcg gttcggatgt
     2641 ccggcaccgg cctctccaac ctcctcttcg gccttctcgc cctcgtctgc tacggggggg
     2701 cgacgctcct ctacgtcacg tggctccgcc atccgaaggt ctcggccggc cggctggcga
     2761 cgctcctcgc cgcggcggga gcggtcttca acttcgtctt cctctacgtc cgctccctcg
     2821 ccctgaagac cgttccctac ggcgaccttc tcgggtcgat ggcgctcttc gggctcttcc
     2881 tggcggcgat gagcctgatc ctggaggcgc ggcaccacga ccgctccctc ggcgcgttcc
     2941 tgatgccggt atcgctcttc ttcctcctcc actcgttcct ccttcccggg ggaagcctcc
     3001 cgaagcagga gatgcgaggt tcgatcttcg cgttccacgt cacgctcaac atgctgagct
     3061 attcggcgtt cgccgtggcg tgcgccctct cgctcctcta cctcgtcctc ggccggcggc
     3121 tccggagcaa gcggcacgtc ctgaacggtc ccgtctcgcg actcccttcc ctgagctacc
     3181 tggagcgggc cgcgcggacc tccatcggag tcggcgtcgt ctcgctcgcg gccgggctga
     3241 cgatgggttt cctctgggcc tacaaggtct ggcacgtcga gcatcccggg tggctcctcg
     3301 acgccaaggt ctggggctcg gtcgtgacgt tcgccttcta cctctgggtc ttcgtccggg
     3361 cgcaccgggg cgcggccccc gggtcgacgg cgaagctctc gatcgccggc ttcgtcctcg
     3421 tcctcctctc gtacaccgcc gtgaacctct tcttctcgaa ggtccactcg ttcgcctgat
     3481 gcctcccgag atcgtcttcg tcggctggag ccaccagggc gcggatctcg acctgagggc
     3541 gcggctcgcc ttcactccgg agaaggcccg ggaggcgctc gaggggctct tccgcgagcg
     3601 gatcctgacc gagggcgcgg tcgtctcgac ctgcaaccgg gcggaggtct acggcgtcag
     3661 cgaggtcccc gacagcatcg acgccctcgc cgagttcttc tcccgcttcc acggcgtcga
     3721 ggcgagcgtc ctccgcgaaa aggcgttctc ggggcagggc gaggcgacgg tgaggcacct
     3781 cttccgcgtc gcctccgggc tcgactcgat ggtcctcggc gaggcgcaga tcctcggcca
     3841 gatccgcgag gcgcaccggc gcgccgccga ggcggggacc gcccgcgccg tcacgagccg
     3901 gctcttccag agcgccctcg agtgcggcaa gcgggtccgg accgagacgt cgctcggcgc
     3961 gcggcccgtc tccgtcccgg gcatcgccct ctccctcgtc ggccggatct tcgagtcggt
     4021 ctccgactgc aaggtcctcc tcctcggcgc gggcgagacc gtggagctga cggcccgcct
     4081 cctcgccgac gacggcgtga agcagatcac cttcacgaac cgctcccccg agaaggcgaa
     4141 ggcgctcgcc ggctgcttcc acggctggac ggtgccgtgg gaggagaggg cgaaggcgtt
     4201 cgcggaggtc gacatcgtcc tctcggcgac gggggcgacc gagccggtcg tcacgttcga
     4261 catgctcaag ccggtcctcg gacgggggcg gcgacgcgga ccgctcctga tcctggacgt
     4321 cgccgtcccg cgggacgtcg acccgagggt cgaggagttc tccgacgtct accgctacga
     4381 catggacgcc ctcggagagc tcgcctgcga caacgcgaag gaccggatgg ccgacgtccc
     4441 tcaggccgag cggatcgtgg aggagtcgac cgagcgcttc ctcggctggc tcgccgggct
     4501 ctcccacgtc gacacgctga agaccctgcg cgggcggctc gagaaggccc gcgtcgacga
     4561 gctggagcgg tacgccggga agctcgcgca cctctccgcc gaggaccgcg ccgtcgtcga
     4621 gcggatgacc gagacgctga tccacaagat ccttcacaac cccacgatcg gcctgaagga
     4681 gggggacgcc tccgagcgcc tcacccgcgc ggccgtcgtg agggccctct tccggctgga
     4741 cggcggggag taggggccgg accacgaaac cgaggaatgc cttgccacga agccgggggg
     4801 cacggggtgc ggcggatggc gctccccgga gagcgtaggg cgcgccggtc gcagccccgc
     4861 cgaaggacga ggaaaggacg acgatgagcc acggacgacc cctccggatg ggaacgcgcg
     4921 cgagcctcct cgcgcggacg cagtcgggac acgtcgccgc cgcgctgacg aaggcgacgg
     4981 ggcgcgcggt cgaggaggtc ctcgtgagga ccgaggggga ccagctctcg accgaggggc
     5041 ggatcccggc ggccggcgac tccgtcggcg tcttcgtccg cgcgctcgac gacgccctcc
     5101 ggcggaacga gatcgacttc gccgtccact ccctcaagga cgtcccgacc gagtacgccg
     5161 aggacctcct cctcgccgcg gtcccggtcc gggccgacgt ccgcgacgcg ctcgtcgtcg
     5221 cctcgcggca cgacgcgaag gacgtccgat cgctcccgca gggggcgagg gtcgcgacgg
     5281 cgagcccgcg ccgcgtcgcg cagcttctcc ggctccgccc cgacctcgag accgtcacga
     5341 tggcggggaa cgtcgacacg cgcctgcgcc gcctcgagga ggggcgcgcc gacgcgctca
     5401 tccttgcctg cgccggcctc gaccgccttt ccctcggcga gcggatcacg cagaggatcc
     5461 cgctcgacga gctcctcgcc gcgccggccc agggggcgct cgggatctcc tgccggagag
     5521 acgacgctga cgccgcggcc gcgatctccc acctcgacga cccggcgacg aacgccgccg
     5581 cggccgcgga acgcagcctc ctgaacgcac tccgggcggg ctgccgggcc ccggtcgccg
     5641 cgctcgcgac gccgggaggc ggcgggacgc tcgccctgcg ggcgcgcgtc ctctccctcg
     5701 acggctccgc gatgatcgag gacgaggcct ccggcaccgt cgaagatccg ctcgcgctcg
     5761 gccggctcct cgcggagcgc ctcctcgccc gcggagcgga gcagctcgtc acggaggccc
     5821 gagcggccgg ccgatgagga gggtcctcct cctccggccc acgggcggcc ccgtcgtcgg
     5881 cgccgacctc ctcgtcaccc accgggtcgt cccggtccct gcggcgctcg cggagacggc
     5941 ggcgtacacc gccgaggggg cgatcctcgt cgtctcctcg ctgaacgccg tcgacatgct
     6001 gagcgacgct ccggaaggaa acctcctcgt gcgtcctttc cgcctcgtcg tctccgccgg
     6061 ggccgggacg tctcgggctc tcgaggcggc cggcgtcgcg gaggtcgtca cccccgaccc
     6121 gcccggcgcg gtcgggatcc tcgcggagct cgcgcggcgc ggagagtcgg gccggctcct
     6181 ctggccgcgc ggctccgacg ccgacgtcgc gcccctttcg gagctcgcgt cgcgcggctt
     6241 cgaggtgacg gcgccgatcg tctacgtgaa ggagccgctc accgcgctcg acgcccggac
     6301 gctctcggcc ttccgcgcgg gggagtacga cgcgctggcg atcgggtcgc tcgccgcgct
     6361 cgacgtcttc ctctccgcgc tcggcgcttc ggcggcccgg gagctgccgc cggtaagatt
     6421 cggcgtcctc ggtccggaga cggcacgcct cctcgtcgcg cgtggcttcc ccgagccgtc
     6481 cgtccccgcg aagcccctcc tcaccgacct gatcgcgctc ctgaaggagg cctgacccat
     6541 ggcgttcccc ctccaccgct accgccgcct gcgcctttcg gagtcgctcc ggacgatggt
     6601 ccgcgagacg acgctcgacc cggccgactt catcgccccg atgttcgtcg tcccgggaga
     6661 agggatccgg cagccgatcc cgtcgatgcc gggcgtcgac cgcgtctcgc ccgacctcgc
     6721 cgcgaaggac gccgccgagc tcgccgcgcg cggcgtcctc tcggtcatcc tcttcggcat
     6781 cccggaccac aaggacgccg tcggctcgcc ctccgccgac ctctccggac ccgtctgccg
     6841 cgccgtcgag gcgatcaagc gggcgagccc cgcgaccgtc gtcatgacgg acgtctgcct
     6901 ctgcgagtac accgaccacg ggcactgcgg cgtcatcgtc gaccaggccg tcgacaacga
     6961 cgcgacgctc ccgatcctcg cggcggaggc gctcgcgcac gcgcgggcgg gggccgacgt
     7021 cgtcgccccg agcgacatga tggacggccg ggtccgcgca atccgggacg tcctcgacgc
     7081 gagcggcttc cggaacgtcc cgatcctctc gtacgcggcg aagtacgcga gcggcttcta
     7141 cgggccgttc cgcgaggcgg ccgagtcgac cccggcgttc ggcgaccggc gcgcctacca
     7201 gatggacccg gcgaacgtcc gcgaggcggt caaggagatc ctctccgacg tcgaggaggg
     7261 ggccgacatg gtcatggtca agccggccct ctcgtacctc gacgtcgtcc gggcggcgaa
     7321 ggaggtcacg aacgtcccgc tcgccaccta caacgtctcg ggcgagtacg cgatggtgaa
     7381 ggccgccgcc gagcgcggct ggatcgacgg caagcgcgtg acgctcgaga tcctgacgtc
     7441 gatgaagcgc gcaggcgcgg acctgatcct gacctaccac gcgaaggagg ccgccgagtg
     7501 gctgcaagga agatgaggcg gaaggcggcc gcgccgccgc ccgcgccgaa gaagaaggcc
     7561 ccgaagaggc ccgtcgcccc ccgccgccgc cgcgtccggc ggaccgtcga gtcggaccgg
     7621 ctctacgccc gagcgaaggc gaagatgccg ggcggcgtca gctcgcccgt ccgctcgttc
     7681 aagtccgtcg gcgccacgcc gttcttcgtg aagaaggcga agggctcgat cctcgtcgac
     7741 gccgacggga accgctacgt cgactacgtc atgtcctacg ggccgcacct cttcgggcac
     7801 atgccgaagg cggtcgcctc ggcgatccgc aaggccctcc cgcgggggac ctcctacggc
     7861 gccccgtgcc gcgccgaggt ggagctcgcc gagcgcgtga cacgcctcgt cccgtcgatg
     7921 gagaaggtcc gtttcgtctc gtccggcacc gaggcggcga tgagcgcggt gcgcctcgcg
     7981 cgggcgatca cggggcgcga cctcgtgctc aagttctcgg gctgctacca cggccatgcc
     8041 gactcgttcc tcgtcgcggc cggctccggc ctcgcgacgc tcgggatccc cggctcgccg
     8101 ggcgtcccgg ccgagctcgc gaaggcgacg atcaccgcga cgtacaacga cctcgagtcc
     8161 gtcgaggccc tcttccggaa gtacccgggg aagatcgccg tcatcgccgt cgagccggtc
     8221 gccgcgaaca tgggcgtcgt cctgccgaaa ccgggcttcc tcgagggcct ccggaagatc
     8281 gcggaccgcg agggcgcgct cctcctcttc gacgaggtca tcaccggctt ccgcctcgcg
     8341 agaggcggcg cgcaggagct cttcggcatc aagcccgacc tgacctgcct cgggaagatc
     8401 ctcgggggcg ggctcccggt cggcgcctac ggcggccggg cggacctgat ggcccacgtc
     8461 gcccccgacg gccccgtcta ccaggcgggg acgctctccg ggaacccgct cgccatggcg
     8521 gcggggatcg cgatgctcga ctcgctcact cccgcggcct acgcgaagct cgagcggacc
     8581 gccgcgaagc tcgagaaggg gatgcggaag gtcgcgaagg agctcggcgt cgaggagcgc
     8641 ctcacgctga accgcgtcgg atcgatcctc acgctcttct tcaatcccgg acccatcgcg
     8701 agcttcgacg acgtcaagaa gagcgacggc gcggcgttca accgctggtt ccacgggctc
     8761 ctcaagagcg gcgtcttcat cgcgcccgcg gcgttcgagg cgatgttcgt ctcgaccgcc
     8821 cacacggaga aggacctcgc gaagacgctc gcggcgcacc gcgaggcgct gaaggaggcc
     8881 ttcgcggaag gctgaggacc gagggcgaag cgcctcgccc gagttcgtcg ggcgaggcgc
     8941 ttcccgggcg gcctggatct acttcaccat cggaacgaag gcaccgtctc ccgtgcgctc
     9001 cgcagctgcg gcgccgtcgt tcacgacgcc gtacgccacc caaggtgcgg tgcccttcgt
     9061 ccgctcgacg cgaacccatc cgtttctgac gttcgacgtg cgaaggaagt tgccgatctg
     9121 agtccacccg cccggctcga gggtccgata gaccctttcc ccggcaggtg ccccgccgac
     9181 ttcgccgtcg aagcactgaa acgccacctc gattgcgccg gagcccgtgg gtcccgtgtg
     9241 gaccgctgcg acgttggacc ggttctcctc gtccgaccgc aggccgtaga ggaatgcctc
     9301 gctctttgcc tcttctcccc cgaggacgcc cggaaggtag agcccgaact gtccctttcc
     9361 tgagggcgtc cgggccgcaa cgcgcgcacc cgccagaatc gatgcggccg gtacgccttc
     9421 gacctcgatt cggagggaac caacgtagct gctcatcgag tatctgggcc cgacgcctcc
     9481 cgccttctgc ctgagccagt cgagagcgtc cgggaacctg gcggcctggc cggcggggag
     9541 gtccagccgg aacgtcccgc ccggcccggc cgacggcgtc gccgactcca cgtagcggat
     9601 cgtggcgacg gcgtcgaccg aagccgtgtt cgcgacgaag agctcgctct ggaagccgct
     9661 ggtctcgacg agcacgggga ccgtcaggta ggtcgccgac gccgagccgg aggccacggg
     9721 aggcacgaac gacccgtcgt tcgtcacggc gtcgttgatg acggcgtacg ccgtcagacc
     9781 cccggtcgac gacgtccggt cgatcttcgc ccaggccgtc gtgaggccgt tcgcctccag
     9841 gagctgagcg gagccgtact ggatccagcc gtacggggga agcgtctcgt cctggcggaa
     9901 gacgaccgtt cgaccgggct ccgtcgccga gaacagggtg atgcgaatcg tcatcggctc
     9961 cgcggacgtg ttgacgacgg cgaggttcgt cctgtcgacg ccggcggcgt tgcgaagagg
    10021 gaagagcgtt acggaggcgg gcgaggctgc cgaggcccgg acgccgccgt acgcgaggcc
    10081 ggcacgtccc acgggcagcg gcgccagggt ggccgacgtc gtgcgagccg tcgccgcgac
    10141 gttcgtctcg tccactgcgg gaccgacgaa tgcgaccttg agcgtacctc cctgctgggg
    10201 atctgccttc gcggtcggca gaggcaaacc cgcggaacgc aggtaaccga gaacgtcgtc
    10261 gatgcgccgc tgctcgccgg cgccgagcgt gagtgtcgtc gaacccgatc ccgctttcga
    10321 tccgagagtc gcggtgtagg ccagcgttgc ggtcgccggt cccgaagacc ggttcgtcag
    10381 cgccagctcg ctcacgtaat gcgccgatcc ggtgtcgacg tcgaggacga tgggcacgac
    10441 ccgggcggtt ccctccggcg cgcaggcatt cgcattcagg aaggccgtcc cgtttgtgag
    10501 gatgtcgggg gcgcgatcgt cattgaggtc cccgagagtg acgggcgaga agcgggtggg
    10561 accgagcgtc gagagaagga ggaggggagc gaagccgcca tcgcgcttgc ccgcgaggac
    10621 ggcgaccttc atctcggcgg agtcgttcga ggcgacgagg tcgggcaccc cgtcgcgatt
    10681 gaggtctccg accgcaaggt tccaggggtt cgtcccggca gggcttgcga cggcggcttc
    10741 gaaagacccg tcgttctttc ccttcatgaa gaagatgctc gaggcgggcg tcgggctccc
    10801 ataggaaccg atggcgacgt cgagaatccc gtcgcggtcg aagtcggcca gcgcgaggcc
    10861 ctgggcggcg gtaccggagg cgtggggcag agagcgtcgc cagggcgcgg cgaaggctcc
    10921 cgcaccgtcc cccagcagga cgacgagctc gggcggatag agaaccgcca cggcgtccgg
    10981 cctcccgtcg ccgttcaggt ctcccacggc cgcgttcacg tattgcctgt ccgtcacgtc
    11041 gtcgagggtc cgctcgggac ggaacgtgcc gtccccgttg ccgggataga gagccaggcg
    11101 agttgcggcg gaaggcatga cgtagggaac gataaggtcg gctttcccgt ctccggtcgc
    11161 gtcaccgatc gcgaggacct ggaatcgtgc tgactccccc acggcgagcg gagatccctt
    11221 gtcttcaaac ccgaagtagt catcgggatc gcctcctgtg ggccccccgc ctccaggccc
    11281 accagcgagg ctcctgttca ggaggacttt gctgcgcggt cccgagcccg acgccacgag
    11341 gtcggtacgg cggtcgccgt cgaggtctcc cgcgaagagc ccccagacgt tgccgacgcc
    11401 ggcactggcg gtcccgagcg gcacgaatga gccggcagcg tcccgcttga agaagcggat
    11461 ggccgcggtc gttcccgttc tcgcgacggc gacgtcgttc gccttcccgt caccgtcgaa
    11521 gtccccggcg acgatcaggt ccgtgtcggc cgccacgacg acgcccggcc cgtaggaggg
    11581 ctgcgggcat tccgcccgtg ccgatccggc gaatgccacg acacagaacg ccacgacgag
    11641 gggcgagagc cgaagaagtc ggcgacactt cctgtccatg tatggctcct tttcggagga
    11701 atgcctgccc gtagtttatt cctctcggct ttggacaggc agatgagacc gtccgctcga
    11761 accgagagca gccggggaga gcccccttgt cgggggtcga cgtcactgac gcccgagacc
    11821 cacgggaggc cggagagatc ccaggcttcc cttccgcgcg tgagcgcgac ttcgaacagg
    11881 ggcacggcgc ttcgccgctc cgggcggtcc gaccggatct ggcctatcgt ggatcagtga
    11941 cgcgccgccc ctggaccccg ccggagacca tcgagacgcc gcggcttcgc ctgcgtcgcc
    12001 cgcgcgccga cgacgccgag gccgtcttcg cgtacgcgcg cgatcccgcc gtgacccggc
    12061 tgatggactg gccgacgcac gtctcggtcg agacgagccg ccgcttcctc gcgttctgcg
    12121 agacgggctg ggaaacgggt acggagttca cgtggctgtt gacgctccct ccctcggacc
    12181 gggcggtcgg cgcgatcgcc tgccgtccga aggagcgcgg cgtggagctc ggctacgtcc
    12241 tccaccgcga cgtccacggc cgcggcctgg cgaccgaggc gtcgcgcgcc ctcgtcgggt
    12301 ggctgacgtc gatgccggac gtcgtgcgca tcgaggcgac gtgcgacgtc gagaacgagg
    12361 cctcggcccg tgtcctcgag aaggcgggcc tggcgcgcga gcggctcctc ccggcgtatg
    12421 gggcccggcc gaacctcggc cccgacccgc gcgacgcgta cctcttcgcc tggacgcgtc
    12481 cggcggactg atggccggaa gcgcttcgaa aggagactcc gggaacatgg ccctcagcgt
    12541 tttcgacgac ccgaaccagc cgccggagcc ggcggtcctc cgcagacggc tcggccccgt
    12601 ggcgacggcg tggagcgcgc tcgtcgacgg cgtcgccgag cgctgcggcc cgatcgagga
    12661 gctctggaag tacggaggcg cgaggttcgg ctggtcgctc cggctcctcc ggaaggggcg
    12721 cgtcctcctc tacctgaccc cgctcgaggg ccggctcctc gccggcctcg tcctgggcgg
    12781 aaaggcggtc gcggcggcgc gggaagccgg tctgccgccg gcgctcctcg ctcaggtcga
    12841 cgcggcgccg cagtatgccg agggccgggg tctccgggtc gaggtttcag ggcccgacga
    12901 cgtggagggc ctccttctcc tcgtcggcct caaggcggac ccgtccgtcc cgccgccggt
    12961 caccgcccag agcccgtccc gagccgggcg accgcgcggt ggcgggggca tccgacgagg
    13021 tggtcgttga cgaccccgac agcctgcagg taggcgtaga cgatcgtcgg gccgacgaac
    13081 cggaagccgc gaatccggag ctccttggag atcgactccg cgaggggcgt cttcgcggga
    13141 acttccccca tctcccggaa cgctccctgg acaggcctgc cgccgacgtg cgcccagagc
    13201 caggaggaga agctcccggc ctccctctgc agcgcgaggt acgccttcgc gctgtcgatc
    13261 gcggattcga tcttgaggcg gttccggacg atgccggcgt cgccgaggag gcgcttcacg
    13321 tccccaggac cgaagcgcgc cactttctcg gggtcgaagc cggcgaaggc cctccggtag
    13381 ctctcgcgct tgcgcaggat cgtgagccag gagaggccgg cctgcgcccc ttccaggacg
    13441 agcatctcga agaggtgcct ctcgtcgcgc gaggggactc cccactcctc gtcgtgatac
    13501 gaaacgtaga gcgggtccga gcccgcccag gggcaacggg cgccttcggg catgccgggg
    13561 atgatctcac caggaccggc gtggggtggt gcgttccggg gtcacttccg tccgatgcgt
    13621 ccgatcgtcc gcgacctcgc cggagaagcg atcctccacg gatcgctccc gggtcggctc
    13681 ttccggaccg ccctcgccgc cctgctcgcc gcctccgcgg ttctcctggc cggttgagca
    13741 gggcgggtcc gtctcagtcg tcgagagcct ccgcgaggag cgtgccgagc cgctcgtact
    13801 ccgccctcgc gttgtagagc tgggccgaga cgcggacgag catcccgccc ggagagggcc
    13861 aggggtagag ccacgcctcg acgccgcgct cccggagcca cccgccgagc ccctgggggc
    13921 cgagccgcgt ggctgtcgag ccgggcgcgg ccttcgggag cggcacggac gccatcgacc
    13981 cgaccatctc cgcgggggcg gccggaggga cgtggagcac cttctcgacc gtttcccgcg
    14041 cctcgagggc gagcgcgcgg ttccgcgtca tcacctcggg ccagccgccg ggcagcaggg
    14101 cgccgagcgc gtcgagcgcc gcggggatcg tcagccaggg cgtcgggtca cacgtcccgg
    14161 tccagtcgaa ctcggcccgg aagcgtgccg gcgacgcctc ttcggagtag ccgaggctcg
    14221 tgacggcagg ccgcgcgcgc gtccggaggt cccttcggac gtggaggaac gcggccccct
    14281 tcggggcgca gagccacttg tgggcgttcc ccgtgtacca gccggcgccc aggcggtcga
    14341 ggtcgatcgg caccatgccc ggggcgtggg caccgtcgac gagcgtctcg acgccccgtt
    14401 cgcggagcgc ggcgacgaga cgcgcgatcg ggaagacgag cgccgtcggg ctggtgacgt
    14461 ggtcgaggag cgcgagacgg gtccgggggg agacggccga gaggacgggg ccgacgacgt
    14521 cctcctcgcc tccgagcggg aacgggacgt cggccacgac gaggcgggct cccgtgcggc
    14581 cgcagacgtg cgcgagcgcg ttgcggcagc cggggtacgc gtgcgtcgtg gtgagcagct
    14641 cgtcgcccgg gccgaggtcg agggagccga ggacggcgtt cactccggtc gtcgcgttcg
    14701 ggacgaacgc gaggtcgtcg ggctcggctc cgaggaacgg gccaaggacg gcgcggactt
    14761 cggcgagccg ttccgggagc cgccgcacga ggaagtcgac cggctcgcgc tcgagctcgg
    14821 cgcggagctc ggcctgacgg tcgagcacgg cggcgggaca ggcgccgaag gagccgtggt
    14881 tcaggaagga gacgcccggg tcgagcctcc agaggcgcgc gaagaggctt tctccggacg
    14941 ggaccgcgcg aggcgcccgg ccgtccgcct ccgccgtcat gccaggccgt accgctcgag
    15001 gcgggtatag agctgggcgc gtgtcaggcc gaggagcttc gcggcccggc tccggttgtt
    15061 ccgcgccttc tcgagagcct gggtgacgag ggtcttctcg agggcgtcga ggtcgagccc
    15121 cccgggcgga agctcgatcg ccgaggcgga ggcgacgcgc gcctccccgc tccgcgcgac
    15181 ggcgatcggg aggtgctccg cctggacgag gcccccctcc gagaggatga ccgcgcgctc
    15241 gagggcgttc cgcagctctc ggacgtttcc cggccaggag taggcaagga ggagctcgcg
    15301 cgcctcccgg gagacgccgg gcgcgggccg gccgacgttc cgggagaggt cctccaggaa
    15361 ccgttccgtc aggaggagga cgtcctcggg gcgctcgcga agcggtggga ggtggatctc
    15421 gaagacggag aggcggtaga agaggtcctc gcggaaatcg ccgcgggcca tcgcggcatg
    15481 gaggtcccgg ttcgtcgcgg cgacgatcct cacctcggcc ttcaggacct tcgcaccacc
    15541 gagccgctgg aactcccgcg cctccaggac gcggaggagc ttcgcctgga cgagtgggct
    15601 cgtctcgccc gcctcgtcca ggaagagcgt tccgcccgcg gcctgctcga gccgccccgc
    15661 gcgcgaggca taggccccgg tgaacgcccc cttctcgtat ccgaagagct ccgactcgag
    15721 gagctgctcg gggagagccg cgcagttgag cgcgacgaac gggccggagc ttcgggcgga
    15781 ggcgcggtgg accgcgcgtg cgacgacctc cttgccggtc cccgattcgc ccgtcaggag
    15841 gaccgtcgtc tccgtcggag cgaccttgga agcctggacg aggaccgagc gccaggcggg
    15901 cgaggcgccg acgacgcgac cgaacccgtc gcgcgactcg agctcgtcgg cgagggcgcg
    15961 gacgcggacc tcgagccggg cggcctcctg ccgggcctcc tccgccctgc gggcctcctc
    16021 ggcgagccgc tggtgggaga acgcgagggc gacctggtcg gcgacccgga gggcgaggtc
    16081 cacgtcctcc tcccggaagc tctgcgtctc gcgggcgtgg aacgagagcg ccccgacggt
    16141 cttcccctcg agccggatcg ggaccctcag gctcgaccgg agcccggcgg cgaggaggat
    16201 cgcccggcgg gaggagtcgg ccgggaggtc ggcctccgcg tcgccgacga tctggtagtc
    16261 ggccaccatc tgcgcgcgtt ccgactcggg gatcgggtag ctgtcgggga cccagtcgcg
    16321 atccccggaa acggcccaca tccggttctc cgtccggtcc tccgacagga gcccgagcgt
    16381 catccgttcg tgcgggagga cctcgcgcgc gatcgccgag acctggtcga agacctcgcg
    16441 gacgtcgagc gccctcgcga gggcgggggc gagggaatcg agctcccggt gccgccggat
    16501 cctccgtcgc tcctcctcgg cgagccgctc gtgctcgagg gtggccgaga ggatgtcggc
    16561 gatcgcctgg gccgtcggct cgtcggccgg gccgaacccg cgcggcgcgc gcgaggcgaa
    16621 ccagaggtcc ccgatcggca cgtcgccgcg gacgatcgtc gcgacgagca tcgagcgcat
    16681 ccccgcgtcg aggagctcgc ggtcggcctg gcgggtcgca tcgagggcga cggccgcctc
    16741 gtcgatcctc cgcggcgaag cggcgcccgg ccagaggagg ggggacacgt cgtcccggcc
    16801 gtagcggcgc cccgcctcgg cgccgtccgg cgtcgcgagc ccccacgaga ggagggcggg
    16861 gccgtcgacg atccgcacga cgccggcggc gtcgaacggc aggacgcgac gcgcggcgcc
    16921 cgcgacgcgc gcgaaggcgt ccggaagcga gagcgtttcc gtcacggtcc gggcgatctc
    16981 ggtcaggacg ccgacgagcg gctcgaccgc gcgcgcgtcc ctggggttgg ttggcacgga
    17041 gcgggcattg taccgggcgc gtcccttaga ctctcccggc catgattccc gttcgcgagc
    17101 tcgccgagcg cgtcctcgcg gggatcccgc cggccatcgc ccgcgccatc acctgggcgg
    17161 agaccgcgga cccgcgattc cccgaactcc tcgcccacct ctggccccga acggggcgcg
    17221 cgcgggtcgt cgggctgacg ggcgctcccg gcgccgggaa gtcgaccctg acggccgccc
    17281 tcgcgaaggc gtcgcgcgtc gagggacgga aggtggggat cgtcgccgtc gacccctcct
    17341 cgcccttcac gggcggggcg atcctcggcg accggatccg gatgcaggag ctctacaccg
    17401 acccgggcgt gatgatccgg tcgatggcga cgcggggtca cctcggcggc ctcgcccgcg
    17461 ccacggccga cgccgtggac gtcctcgacg cggcgggctt cgacgacgtc ctcgtcgaga
    17521 cggtcggggt cgggcaggac gaggtcgagg tcttccgtct cgccgagacc tgcgtcgtcc
    17581 tcctgacgcc ggggatgggc gacgacatcc aggcgatcaa ggccgggctg atggaggtcg
    17641 ccgacgtctt cgtggtcaac aaggccgacc gcgagggggc cgaccggacc gcccacgagg
    17701 tccttcagat gctcgagatg ggcgatcacg gcgccttcct gccgccggtc ctgaagaccg
    17761 tcgcacggac gggcgaaggg gtcgcggagc tccgggccgc gctggcgagc cacaaggagt
    17821 tcctcttcgg gccggcgggc gcgtcccgcc gccgggaccg gaccgtccga cggatcgaag
    17881 ccatcgtcag cgaccgtctc ctcgccgagc tccacggcct cgccgggagc gacgcctccc
    17941 tcgcctcctg ggcggggagg gtcgaggcga ggagcgaaga cccgttccgc gccgccgagg
    18001 ccctcctcga gacgctgcgg aagaacggcg cccgaagcgc gccgaccgga ggaaagatga
    18061 agatctcgca cctcggcctc gccgtcccgt ccgtcgacgg cggcggcgcc atctggga
//
LOCUS       DAOTEZ010000024        17787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2129_length_17787_cov_6.263591, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000024 DAOTEZ010000000
VERSION     DAOTEZ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17787
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2129_length_17787_cov_6.263591"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /locus_tag="P5164_02245"
     CDS             complement(<1..1279)
                     /locus_tag="P5164_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRY42742.1"
                     /translation="MRTLPIVLALSLAAPAGLAAPAKAAAKDAKAAAEKPKEPYSAES
                     FAGLALRDLGPALTSGRVGDLAVDPVRKGTYYVVVASGGVWKTTNWGTTFEPVFDSEG
                     SFSIGCIALDPTNPLVVWVGTGENNSQRSVGYGDGVYRSPDGGKTWAKVGLEASEHVG
                     KILVHPKDGKTVYVAAQGPLWADGGDRGLYKTTDGGKTWKAVLSISPKTGVSDVWMDP
                     RDPEVLYATAYQRRRHVFTLINGGPESGIHKSTDGGATWTKLTNGLPKGDVGRIGLAI
                     PPTAPDTVYALVEARGKDGGFYRSLDGGMSWEKRGEYATGSAQYYQELFPDPHDASRV
                     YSMDVYMQVTEDGGKTWRDAGEKAKHVDNHALWIDPENPDHLLCGGDGGIYETWDRCA
                     TWQFKANLPVTQFYRVAVDDAKPFYNVYGGTQDN"
     gene            complement(1284..3425)
                     /locus_tag="P5164_02250"
     CDS             complement(1284..3425)
                     /locus_tag="P5164_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRY42743.1"
                     /translation="MKQIPTDLVPALLMVCAFAAVLVPREAGAQGRVGYPATRAVDAK
                     DVLHGVTIPDPYRWLEDAKDPEVQAWMRAQDDLTRKELARVPERARLLARLKELAYLD
                     SVSAPTKRGSRLFLTRRHATKEKSVVYWKEGKAGEEKVLFDPNGWSKDGSAGLGDWQA
                     SHDGTKVVYQLKENNSDEATLRVIDVATGKVSEIDVIPGGKYATASWTPDGSGFYYTW
                     LPTDPKIPVDERPGHQEVRFHRLGEDPAKDRVVHGKLGDPTAFLSATLSRNGRWLVLY
                     VQYGWTSNDVLYRDLADPTQADRWTPLVVGTEAQWGVEVFGDRFFVTTNDGAPRSRVF
                     EVDPLRPARKEWKEVVPERKDATLVSASVLGGRLALNYLKDASSLLEVRELDGALVRE
                     VKLPGLGTVDGPFGLPDEDEAYYSFESYTTPREIHATSMKTGVQELWFRLTVPVDPSA
                     YEVEQVFFPSKDGTRLSMFLVHRKGMKRDGAARTILYGYGGFQVSETPHFSAMLYPWL
                     EEGGVWAAVNLRGGGEYGEEWHRDGMLLKKQNTFDDFLAAARWLSAEKVTSPERLAIY
                     GGSNGGLLVGAAMTQEPKAFSAVLCAVPLLDMVRYHLFGSGRTWISEYGSADDPKQLA
                     ALLAYSPYHAVKTGTAYPATLLLSADSDDRVDPMHARKLAAALQAATTGGPVLLRIER
                     NAGHGGADMVKSAVESRADEYAFVLAHTKGQ"
     gene            3518..4837
                     /locus_tag="P5164_02255"
     CDS             3518..4837
                     /locus_tag="P5164_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42744.1"
                     /translation="MVRLCARLAFALLVAAPLAAAVGRPASPTTAPSEAAAPGGQAFA
                     LALGGSGSVLASGPYALRVPGAEGPLRLELARLAVDGARLRGEARLTNGSGSLLSGLE
                     LDFRTVTTTVRAVPGRAAPEPAPLSLSRPLSLGELLPGEATPFVPFEVAPLPLGEDVT
                     VVVLLGAVTGLAASPPISVEGALRPVALDVDRSGRLYVATSGVGRVLRLTAASAASPG
                     EAARPSAPPTGVALRRRDQALFVATGGPTVEVHRPGRARPATLDAGRPVTLLRVDASG
                     VLRAASGNGVLAFDEAKAGPPAPLGPEGSAVLSFDADARGRVHAVVRNGETRQVVVAG
                     TAGASPLAAGRGPGADALLSPRALRFAADGTTWVAAEATAPEGAALTRFSTDGTPLGT
                     LPRLGLALLLGKDEDAAVPAIVDLAPGPDGLLWVLLESGALFGVRPL"
     gene            complement(4887..5309)
                     /locus_tag="P5164_02260"
     CDS             complement(4887..5309)
                     /locus_tag="P5164_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42745.1"
                     /translation="MEITRKSFLSGAAAGLAGLSLLPAAAWAAPATETETGILWLRNA
                     AGQTFTARSADGALVTLLLEKVEELKSGTDVEQYRLLFSADGNYRLAEGTWRLGGSRG
                     RRAGDVFLVPAGTNAAGRALYRADFCLLAKLVPETPRR"
     gene            5426..5734
                     /locus_tag="P5164_02265"
     CDS             5426..5734
                     /locus_tag="P5164_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42746.1"
                     /translation="MTETLSISDFTPLVGAAFTIRFPDGTLELTLDAVEPRGVRAPRP
                     EIPDLRTEPFSLVFLGPLHPVLPQQTWDLSHPALGTRSIFLVPIGPKEGGMRYEAVFN
                     "
     gene            complement(5727..6257)
                     /locus_tag="P5164_02270"
     CDS             complement(5727..6257)
                     /locus_tag="P5164_02270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005372124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY42747.1"
                     /translation="MERSAMAERGEMAETRVPRALTLRPVTPADRELLFRVYASTREE
                     ELAPVPWPPEAKEAFLRQQFDAQDAWWRANYAGATFEVIVVAGVDSGRLYLWEGPKEI
                     RVVDVALLPEARRGGSGTRLLEGLKARARAAGKAVSIHVERMNPALALYERLGFRLVE
                     DKGVYLFLSWSADAVS"
     gene            complement(6274..6795)
                     /locus_tag="P5164_02275"
     CDS             complement(6274..6795)
                     /locus_tag="P5164_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HRY42748.1"
                     /translation="MADPFVAEIRIFPFNFAPKGWAFCDGQILPLSQNTALFSLLGTT
                     YGGDGKSNFGLPNMQGNAPMHPGQGPGLSLHELGETGGSDTVSLLESEIPAHAHTLRA
                     DVLDIADTNVVSPTASFALSSGGTLYQSTANVSLSGNALAPAGGDQPHNNMQPYLTLN
                     FCIALQGVYPPRT"
     gene            complement(6823..7326)
                     /locus_tag="P5164_02280"
     CDS             complement(6823..7326)
                     /locus_tag="P5164_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HRY42749.1"
                     /translation="MAQPYVGEIRMFAGNFAPAGWMFCEGQLLPISEYETLFNLIGTT
                     YGGDGQSTFALPDLRGRVPLHFGNGFVLAETGGVETVTLTVSQIPAHTHAVLASTATG
                     DQVSPAGNVPATSFNVTPYINDAANGNFSASAVTSTGGSQPHDNFQPYLCVDFIISLF
                     GIFPSPT"
     gene            complement(7337..7837)
                     /locus_tag="P5164_02285"
     CDS             complement(7337..7837)
                     /locus_tag="P5164_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HRY42750.1"
                     /translation="MAEPFLSEIRMMSFGFAPRGWALCNGQLLPINQNQGLFSLLGTT
                     YGGDGRVNFALPNLQGRVPIHMGSGHTLGERGGEQAHTLSISELPTHVHSVQASSAAT
                     GGNANPTGRVLGGGNNVYHAPTSLTSMTPGTLANVGGSQAHLNMQPFLVLNFSIALQG
                     IFPSQT"
     gene            complement(7872..16088)
                     /locus_tag="P5164_02290"
     CDS             complement(7872..16088)
                     /locus_tag="P5164_02290"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HRY42751.1"
                     /translation="MTYRTALGLVLAAALGTSTLLVAETTYLPGAAELAGLQGARFSS
                     TLELTNPGEEAATATIGLVPMAGKTPPAPVTRTLAAGESVRIEAALKTLFGLADDGAG
                     TITVLSDAALLASLTTRNVAAAEGPYGLGLFAVPEADLLGAGETGHSIWVSHSADPKT
                     GYRTNLSVTLVDPGTIVVVRVFDSDGSLAGETTLSAQTPQVWQKPVTAIAGESSLPVG
                     RAEFEVKAGHATAYAVVNDNVTSDAIALPCERVPSGATERLVSGAALSPGLLGAFWVT
                     DLRIFNPGAEPVVATIRSLGAPTAASAVVPIPAHGVVEVPRVLALLGFPERTASALLV
                     SAEAPLLVAARTNNVDPAGVRKGTFSAQQFVSVWPTGLLAAGATGFFTGIDQTLNVPG
                     TRTNLALVGGPEGAAGALVLRDERGAEQGRTPFRRAPGEWGQLGVADWFASSLAGRTS
                     RDGLSLATVPPNSRIDVTVEQGALDAYVSQIDNGSGDAVTRPFGLPGGGDCTKVAIVA
                     FEATPLPVKPGVETTLSWTVKLDPPTAELTSQSLRIGAGPEVELDKDVRSYATSFPEA
                     GPVAVTLTVRKGSCVKTRTLQIAVCGDLGVEPTALVAATVGEPYAGATFTAPAGRPPL
                     AFRISAGALPAGLVLTAAGLLEGTPAEAGVASFSVAVTDANGCAGERAYTIEVGCPPM
                     SISPTDLPGGTVGTPYAMVVLSVTGGSGSGTFEGRRLPPGLTVSSNGRIEGTPTEAGR
                     YDSEIVYRDANGCDVTVPRPIVVCNPISVTATLPAATAGVAIAPTPFSVSGAVGATTF
                     AVTAGALPAGTTFDGAAGVLSGTPTVVGTFPFTVTATDSLGCTGSLATSLVVSCPTIT
                     VSPASLSNGTAGVTYGAVTLTQAGGVGTATYAVTAGALPAGLTLSSAGVLSGTPTVTG
                     TFPFTVTATDANECTGTQAYTLVVECPVITVSPATLGNGTAGVAYGPVTLTQAGGVGA
                     ATYAVTAGALPAGVTLTTAGVLSGTPTVTGTFSFTVTATDANGCTGTRALTLVIDCPV
                     IAVSPASLANATAGAAYGPVALTQTGGVGAVTYAVTVGALPTGVTLTTAGVLTGTPTV
                     TGTFSFTVTATDANGCTGTRAYTLVVECPVITVSPATLPGGTTGIAYIQSVGSSGGLG
                     TMTYAVTTGALPPGLVLNGVSGAITGTPTAAGTYDFTVTATDGNGCTGSQAYTVGIVC
                     ATITVGPGTLVNGTVGTAYSQTVLGAGGGGAYLYAVTAGTLPAGLSLDGASGAITGTP
                     TTADLFSFTITATDQYGCSGATAYQVRICPVIEVTPATAAQGTIGQVYAGTTFAQTGG
                     VLPVAWSATGLPAGLALDAATGALTGTPTEAGGFAVVVTATDGNGCTGSVAFTLRICP
                     EMAINAPASLAAGTVGTAYGPVTFTQAGSGATIAWSATGLPTGLALDPTTGVLSGTPG
                     EPGSFTVVVTATDANGCAVSRSYPLTITCPTITLSPGALPGATAATAYSQTVTASSAL
                     AGGSYSYSILSGALPAGLTLNPATGEISGTPTAVGTFNVTIRATHDPTNCTGDQAYTL
                     VVACATITVGGGAPPTNVTAGVAGYTHTFTATGGVGPWTFSATGTLPTGLTLNPTTGV
                     LSGTPTAAGTYNFTIVATDSATGATCTGSAPFSVTVVCPTITVSPATLPGGIVGTAYP
                     ETATAAGGTGPYTFAVTAGALPTGLTLGTNGAITGTPSAEGEFTFTITATDTTTSCTG
                     SRAFTVRICPVIGLAAVPTCATVGSTYTTTITPSAGTAPFGFTSTGTLPPGTTLNPTT
                     GVLSGPLSAAGSFSFSITATDVNGCAGSQSYTVNVLDMTPAAGVLPDATFGTAYSQAI
                     TASGGSGGFTYAVTAGSLPEGLSLNPATGALTGTPGGALGTGSFSFTVTATNTATTCT
                     IAKAYTLVSRPVAVADAYANGAGNTEYYAGGYTPTPATPFVASATNILSNDLGPGLTV
                     TSLTQPANGTVTLGAAGAFRYTPNAGAETSATFTYKVTSNGVESQTAASVTITLVGQV
                     WYVNSSGANGIGTSNSPFNNLANADVSSGAGDVVFVHTGAATTTGAIILDANQVLWGQ
                     GAAFSLGGSGLTIGAAGKPTLTGTVTLGGSSVLVSSLDIATTGAVGFTDNQAGAITGI
                     TVQNSVTVSATNAAAVDLTGVSASASGITFTSLTSSNSTGKGLNLVGIGGTFTSGTTT
                     VTNPAGIGIDVQTSVAGGTISLGTTSTTLSGGTGVNLASNAGNVTFGALTVSPDGNQR
                     GLLATENAGTLTVPSGTITTTGATAVEITRTAGTTPLAVTLTAVNANGGPNGIYLSRT
                     SGSFTVAGTGTAGSGGTIRNQAGADGAVAGSGVYLSNVSNVSLSRMQLNDFPNFAIRG
                     IGVSGFSLTNSVVNGANGTNGAIDEATIAFDGLTGTALIQNTSISGGVEDNFRVRNSS
                     GTLNRITFDATTFGANNAATGNDALLLEPSGAAVLNVTVQNCTFTSAAGDLFQLNLLG
                     SSVSDLVFTGNTLSNNHPAIATGGGGVTITGGDSTTPSGVTLTYNIANNTFRDANGHA
                     VLIVKSTDPGSFTGTFTNNTIGVAGLANSGSVAGSGIKLQTAGLGTFSAYVTNNQIRQ
                     YNNFGIELLTGGGASAMSGSFNAKVTGNTVSNPGTGGLPMNGIHLNGGTVPGDTFAIC
                     VDIGGAGALANTINGSGANGGTDVRLRQRQSTTVRLPGYGGANNDNTAVQNYLIGRNV
                     ASPTALAENTVASGVGGFIGGAPCLP"
     gene            16415..>17787
                     /locus_tag="P5164_02295"
     CDS             16415..>17787
                     /locus_tag="P5164_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HRY42752.1"
                     /translation="MSRRKLIGLGAATVAATALPDALFRLSSAAAATGPTEGIGYFAR
                     FGVTEKMLRDGLSAALSRGADYADLFFQHRVGNSLALEDGEVNRAYASVELGVGVRAV
                     KGDQTGYAYTEDLTPEALVQAAKTAAAIADGPAKPGPKGLKADTSLPSRYPVKTRWED
                     VRPQQKLPLLSGLNEKVFAADKRITKVNVFFGDEAGAILIADSTGRVVEDLQPMTRLG
                     LSCVAEQDGKKETNGYNVAGRADLGYYSPDRLERIVKEAVSRTVILFEAVQPPAGEMP
                     VVLGAGASGILLHEAIGHGMEADFNRKGISIFADRIGKPVANKFVNIVDEGTIAGARG
                     AINVDDEGNPVGKTMLVENGILTTYLHDSISAKHYKVKPTGNGRRESYAYAPVPRMRA
                     TYMLPGPHKKDEIVASVKKGIYCTNFTNGQVQIGAGDFTFYVKNGYLIEDGKLTTPIK
                     DVNIIGNG"
BASE COUNT         2402 a         6408 c         6283 g         2694 t
ORIGIN      
        1 tgttgtcctg cgtgccgccg tagacgttgt agaagggctt cgcgtcgtcg acggcgaccc
       61 ggtagaactg cgtcaccggg aggttcgcct tgaactgcca cgtcgcgcag cggtcccacg
      121 tctcgtagat gccgccgtcg ccgccgcaga gaaggtggtc cgggttctcc gggtcgatcc
      181 agagggcgtg gttgtcgacg tgtttcgcct tctcgccggc gtcccgccac gtcttgcccc
      241 cgtcctcggt cacctgcatg tagacgtcca tcgagtagac gcggctcgca tcgtgcgggt
      301 cggggaagag ctcctggtag tactgcgccg agccggtcgc gtactcgccc cgcttctccc
      361 agctcatccc gccgtcgagc gagcggtaga agccgccgtc cttcccgcgc gcctcgacga
      421 gcgcgtaaac ggtgtcgggc gcggtgggcg ggatcgcgag gccgatccgg ccgacgtccc
      481 ccttcgggag gccgttcgtc agcttcgtcc aggtcgcccc gccgtccgtc gacttgtgga
      541 tcccgctctc cggaccgccg ttgatgagcg tgaagacgtg ccgccgccgc tgataggccg
      601 tggcgtagag gacctccggg tcgcgcgggt ccatccagac atcgctgacg cccgtcttcg
      661 gcgagatcga gaggaccgcc ttccacgtct tcccgccgtc cgtcgtcttg tagaggcccc
      721 ggtcgccgcc gtcggcccag agcggcccct gcgcggcgac gtagacggtc ttcccgtcct
      781 tcgggtggac gaggatcttc ccgacgtgct cggacgcctc gagccccacc ttcgcccacg
      841 tcttcccgcc gtcgggcgag cggtagacgc cgtcgccgta gccgacgctc ctctggctgt
      901 tgttctcgcc ggtgccgacc cagacgacga gcgggttcgt cgggtcgagg gcgatgcagc
      961 cgatcgagaa cgagccctcg gagtcgaaga ccggctcgaa cgtcgtcccc cagttcgtcg
     1021 tcttccagac gccgcccgag gcgacgacga cgtagtacgt ccccttgcgg acggggtcga
     1081 ccgcgaggtc gccgacgcgg cccgaggtga gcgcggggcc gaggtcacgg agcgcgagcc
     1141 ccgcgaacga ctcggccgag tacggctcct tcggcttctc ggccgcggcc ttcgcgtcct
     1201 tcgccgcggc cttcgcgggc gcggcgaggc cggccggggc ggcgagggag agagcgagga
     1261 cgatcggaag ggttctcatg gcgtcactgt cctttcgtgt gcgcgaggac gaaggcgtac
     1321 tcgtcggccc gggactcgac ggccgacttc accatgtcgg cgccgccgtg cccggcgttc
     1381 cgctcgatcc ggaggaggac gggcccgccc gtcgtcgcgg cctggagggc cgccgcgagc
     1441 ttgcgggcgt gcatcgggtc gacccggtcg tcgctgtcgg ccgagaggag gagcgtcgcc
     1501 gggtaggcgg tccccgtctt cacggcgtgg tagggggagt aggcgaggag tgctgcgagc
     1561 tgcttcgggt cgtctgccga gccgtactcg ctgatccagg tccggcccga gccgaagagg
     1621 tggtagcgga ccatgtcgag gagcggcacg gcgcagagga cggccgagaa ggccttcggc
     1681 tcctgcgtca tcgcggcacc gacgaggagg ccaccgttcg agccgccata gatcgcgagc
     1741 cgctcggggc tcgtcacctt ctcggccgag agccagcgcg ccgccgcgag gaagtcgtcg
     1801 aacgtgttct gtttcttcag cagcatcccg tcccggtgcc actcctcgcc gtactcgccg
     1861 ccgccgcgga ggttcacggc ggcccacacg cccccctcct cgagccacgg gtagagcatc
     1921 gcggagaagt gcggcgtctc gctcacctgg aagccgccgt agccgtagag gatcgtccgg
     1981 gccgccccgt cccgcttcat tcccttccgg tggacgagga acatcgagag gcgcgtgccg
     2041 tccttcgacg ggaagaagac ctgctcgacc tcgtaggccg acgggtcgac ggggaccgtc
     2101 agccggaacc agagctcctg cacgcccgtc ttcatcgacg tcgcgtggat ctcgcgcggc
     2161 gtcgtgtatg actcgaacga gtagtacgcc tcgtcctcgt ccgggaggcc gaaggggccg
     2221 tcgacggtcc cgaggccggg gagcttcacc tcgcggacga gcgccccgtc gagctcgcgg
     2281 acctcgagga gcgaggacgc gtccttgagg tagttcagcg cgaggcgccc gccgaggacc
     2341 gaggccgaaa cgagggtcgc gtccttccgc tcggggacga cctccttcca ctccttccgc
     2401 gccgggcgga gcgggtcgac ctcgaagacg cggctccgag gcgctccgtc gttcgtcgtc
     2461 acgaagaagc ggtcgccgaa gacctcgacc ccccactgcg cctccgtgcc gacgacgagc
     2521 ggcgtccagc ggtcggcctg cgtcgggtcg gcgaggtcgc ggtagaggac gtcgttcgag
     2581 gtccagccgt actggacgta gaggacgagc cagcgcccgt tacgcgagag cgtggccgac
     2641 aggaaggccg tcgggtcccc gagcttcccg tggacgacgc ggtccttcgc cgggtcttcg
     2701 cccaggcggt ggaagcggac ttcctggtgc ccgggccgct cgtcgacggg gatcttcggg
     2761 tcggtcggga gccacgtgta gtagaagccc gagccgtccg gcgtccacga cgcggtcgcg
     2821 tacttcccgc ccgggatgac gtcgatctcg gagaccttcc cggtcgccac gtcgatcacg
     2881 cggagcgtcg cctcgtccga gttgttctcc ttgagctggt agacgacctt cgtcccgtcg
     2941 tggctcgcct gccagtcgcc gaggccggcg ctcccgtcct tcgaccagcc gttcgggtcg
     3001 aaaaggacct tctcctcgcc cgccttccct tccttccagt acaccaccga cttctccttc
     3061 gtcgcgtggc ggcgggtcag gaagaggcgg ctcccgcgct tcgtcggcgc ggacaccgag
     3121 tcgaggtagg cgagctcctt gagccgggcg aggaggcgag cgcgctcggg gaccctcgcg
     3181 agctcctttc gcgtcaggtc gtcctgcgcc ctcatccagg cctgcacctc ggggtccttc
     3241 gcgtcctcga gccagcggta agggtcgggg atggtcacgc cgtgaaggac gtccttcgcg
     3301 tcgacggcgc gcgtcgccgg gtagccgacg cggccctggg ccccggcctc tcgcgggacg
     3361 aggacggcgg cgaacgcgca gaccatgagg agggcgggca cgaggtccgt cgggatctgc
     3421 ttcatcaggg aacgctcctt gaaacggggg gagtggtcat cacgccaggc ccgtaatcat
     3481 cccacgggga gacgccgggg cggctaccat cggaaacgtg gttcgcctct gcgcacggct
     3541 cgccttcgcc ctcctcgtcg ccgcgcccct tgcggcggcc gtcggccgcc ccgcgtcgcc
     3601 cacgaccgcg ccgtccgagg cggccgctcc gggcggacag gccttcgcgc tcgctctcgg
     3661 agggagcggg agcgtcctcg catccggccc ctacgccctc cgcgtccccg gcgcggaggg
     3721 tccgctccga ctcgagctgg cgcgtctcgc cgtcgacggc gcgcggctcc ggggcgaggc
     3781 ccgcctcacg aacggctccg gctcgctcct ctccggtctc gagctcgact tccgtaccgt
     3841 cacgacgacg gtccgcgcgg ttccaggccg ggccgccccc gagcccgcac cgctctcgct
     3901 ctcgaggccg ctctctctcg gagagctgct gccgggagag gcgacgccgt tcgttccgtt
     3961 cgaggtcgcg ccgctccccc tcggcgagga cgtgaccgtc gtcgtcctcc tcggtgccgt
     4021 gaccggcctc gccgcgagcc cgccgatctc cgtcgagggc gcccttcgac ccgtggcgct
     4081 cgacgtggac cgttccggcc gcctctacgt cgcgacgtcg ggcgtcgggc gggtcctccg
     4141 gctgacggcc gcctcggccg cgtcgccggg cgaggcggcc cggccatctg cgccgccgac
     4201 gggagtcgcg ctccgccggc gcgaccaggc cctcttcgtc gcgacgggcg gccccacggt
     4261 cgaggtccat cgtccgggtc gcgcgcgccc ggcgacgctc gacgcggggc ggcccgtcac
     4321 gctcctccgc gtcgacgcga gcggcgtcct gagggccgcg tcaggcaacg gggtcctcgc
     4381 gttcgacgag gcgaaggccg gtccgcccgc gccgctcggc cccgagggga gcgcggtgct
     4441 ttcgttcgac gcggacgccc ggggccgcgt ccacgccgtc gtccggaacg gcgagacccg
     4501 gcaggtcgtc gtcgccggga ccgcgggcgc ttctcccctc gccgcgggtc gtggccccgg
     4561 ggccgacgcc ctcctctcgc cccgtgcgct ccgcttcgcc gccgacggaa cgacgtgggt
     4621 cgcggcggag gccacggctc ccgaaggcgc cgcgctcacc cgcttctcca cggacggcac
     4681 gccgctcgga acgctccccc ggctcggcct cgcgctcctc ctcggaaagg acgaggacgc
     4741 ggccgtcccg gcgatcgtcg acctcgcgcc ggggcccgac gggctgctct gggtcctgct
     4801 cgagagcggc gcgctcttcg gcgtccgccc cctctgagcc gcccgcggct ccgggcggcg
     4861 gcgcgccccg ctcgccttcc ttcgcctcag cggcgcgggg tttccgggac gagcttcgcg
     4921 aggaggcaga agtccgcgcg gtaaagcgcg cggcccgccg cgttcgtccc cgcgggaacc
     4981 aggaagacgt ctccggcgcg ccgtcccctc gacccaccga gccgccacgt cccctcggcg
     5041 aggcgatagt tcccatcggc cgagaagagg agcctgtact gttccacgtc ggtgcccgac
     5101 ttcagctcct cgaccttctc gaggaggagc gtcacgagcg ccccgtccgc gctccgcgcc
     5161 gtgaacgtct ggccggcggc attcctcagc cagaggatgc ccgtctccgt ctccgtcgcg
     5221 ggggcggccc aggccgcggc cggcagaagc gacagccccg cgagtcccgc ggccgcgccg
     5281 gaaaggaacg acttccgtgt gatctccatg gcactcctca cgccgcgcga cggggggcgg
     5341 cggggccgct gacgcgactt ccccgctccg gcgcgctgga cgaagcgccc ggccgcgggc
     5401 atcctccctc cggccggagg gacccgtgac cgagacgctc tcgatctccg atttcacgcc
     5461 gctcgtcgga gcggccttca cgattcgatt tcccgacggg acgctcgagc tgacgctcga
     5521 cgcggtcgag ccgcgcggcg tacgcgctcc gcgccccgag attccggacc tccggacgga
     5581 gccgttctcc ctcgtcttcc tcgggccgct ccaccccgtc ctgccgcagc agacgtggga
     5641 cctctcgcac cccgcgctcg ggacgcggtc gatcttcctc gtgccgatcg gcccgaagga
     5701 aggcggcatg cgctacgagg ccgtcttcaa ctgaccgcat cggcgctcca ggagaggaag
     5761 aggtagaccc ctttgtcctc gacgagacgg aagccgagcc gctcgtagag cgcgagggcc
     5821 gggttcatcc gctcgacgtg gatcgagacg gccttcccgg ccgcccgtgc ccgcgccttc
     5881 aggccctcga ggagccgcgt gccggagccc ccgcgccgcg cctcgggaag gagcgcgacg
     5941 tcgacgacgc ggatctcctt cgggccctcc cagaggtaga gcctcccgga gtcgacgccc
     6001 gcgacgacga tcacctcgaa ggtcgccccc gcgtagttcg cgcgccacca ggcgtcctgc
     6061 gcgtcgaact gctggcggag gaacgcctcc ttcgcctcgg gcggccaggg cacgggggcg
     6121 agctcctcct cgcgcgtcga ggcgtagacg cggaagagga gctcgcgatc ggccggcgtc
     6181 acgggacgga gcgtcaacgc gcgcggcacg cgcgtctccg ccatctctcc ccgctccgcc
     6241 atagccgacc tctccacgcc cgcgcctccc cggtcaggtc cgcggcgggt agacgccctg
     6301 cagcgcgatg cagaagttga gcgtcaggta cggctgcatg ttgttgtgcg gctggtcgcc
     6361 gccggcgggg gcgagggcgt tgccggagag gctgacgttc gccgtcgact ggtagagcgt
     6421 gccgcccgac gagagggcga acgaggcggt cgggctgacg acgttcgtgt cggcgatgtc
     6481 gaggacgtcg gcccggagcg tgtgcgcgtg cgcggggatt tcgctctcga ggagcgagac
     6541 cgtatcgctc cctcccgtct cgccgagttc gtggagcgac aggcccggcc cctggcccgg
     6601 gtgcatcggg gcgttcccct gcatgttcgg caggccgaag ttcgacttcc cgtccccgcc
     6661 gtaggtcgtg ccgaggagcg agaagagggc cgtgttctgc gagagcggga ggatctgacc
     6721 gtcgcagaac gcccagccct tgggcgcgaa gttgaacggg aagatccgga tctcggcgac
     6781 gaaggggtct gccatggggt gctcctcgtg gctctctgcg gatcaggtcg gggacggaaa
     6841 gatgccgaag aggctgatga tgaagtcgac gcacaggtac ggctggaagt tgtcgtgcgg
     6901 ctggcttccg cccgtcgagg tcacggcgct cgcggagaag ttcccgttcg cggcgtcgtt
     6961 gatgtagggc gtgacgttga acgacgtcgc cgggacgttt cctgccgggc tgacctggtc
     7021 gcccgtcgcg gtcgaggcga gaaccgcgtg cgtgtgggca gggatctgcg agaccgtgag
     7081 ggtgaccgtc tcgactccgc ctgtctccgc gaggacgaag ccgttcccga agtggagcgg
     7141 gacacggccg cgcaggtcgg gcagggcgaa cgtgctctgc ccgtccccgc cgtaggtcgt
     7201 cccgatcagg ttgaagagcg tctcgtactc ggagatcggg aggagctggc cttcgcagaa
     7261 catccagccc gcgggggcga agttccccgc gaacatccgg atctcgccga cgtagggctg
     7321 cgccatctcc gtctcctcag gtctggctgg ggaagatccc ctgaagggcg atcgagaagt
     7381 tgaggacgag gaacggctgc atgttcaggt gtgcctggct ccccccgacg ttcgcgagag
     7441 tccccggcgt catcgacgtc aggctcgtcg gcgcgtggta gacgttgttt cctccgccga
     7501 ggacgcggcc cgtcggattc gcgttcccgc ccgtcgcggc cgacgaggcc tggacggagt
     7561 gaacgtgcgt cggcagctcg gagatcgaga gcgtgtgggc ctgctcgccc ccgcgctcgc
     7621 cgagcgtgtg cccgctcccc atgtggatcg ggacccgccc ctgcaggttc ggaagggcga
     7681 agttcacgcg cccgtcccct ccgtacgtcg tcccgagaag ggagaagagg ccctggttct
     7741 ggttgatcgg gaggagctgc ccgttgcaga gcgcccaccc cctcggggcg aacccgaagc
     7801 tcatcatgcg gatctcggaa agaaatggtt ccgccatctc gctctcctcc tctactcgat
     7861 tctcgctgtc gtcacggcaa gcagggggcg ccgccgatga agccgccgac gccggaggcg
     7921 accgtgttct cggccagcgc ggtcggcgac gccacgttcc tgccgatcag gtagttctgc
     7981 accgccgtgt tgtcgttgtt cgccccgccg tagccgggga ggcgaaccgt cgtcgactgt
     8041 cgctgccgga gccggacgtc ggtccctccg ttcgcgcccg agccgttgat cgtgttcgcc
     8101 agtgcccctg cgcccccgat gtcgacgcag atcgcgaagg tgtcgcccgg aaccgtcccg
     8161 ccgttgaggt ggatgccgtt catcggcagg ccgcccgtcc cggggttcga gaccgtgttc
     8221 ccggtgacct tcgcgttgaa gctcccgctc atcgcggacg cgccgccacc ggtcagcagc
     8281 tcgatcccga agttgttgta ctgccggatc tggttgttcg tgacgtacgc gctgaacgtg
     8341 ccgaggccgg ccgtctggag cttgatcccc gaaccggcca ccgagccgga gttcgcgagc
     8401 cccgccacgc cgatcgtgtt gttcgtgaag gtgcccgtga acgaccccgg gtccgtcgac
     8461 ttgacgatga ggaccgcgtg gccgttcgcg tcgcggaacg tgttgttcgc gatgttgtag
     8521 gtcagcgtca cgcccgacgg ggtcgtgctg tcgccgcccg tgatcgtcac gccgccgccg
     8581 cccgtggcga tggccgggtg gttgttggag agcgtattgc ccgtgaagac gaggtccgag
     8641 acgctggagc cgagcaggtt cagctggaag aggtcgcccg cggccgacgt gaaggtgcag
     8701 ttctggaccg tgacgttgag gacggccgct ccgctcggtt ccagcaagag cgcgtcgttc
     8761 cccgtcgcgg cgttgttcgc tccgaacgtc gtcgcgtcga acgtgatccg gttgagcgtt
     8821 ccggacgagt tgcggacgcg gaagttgtcc tccacgccgc cgctgatgct ggtgttctgg
     8881 atgagggccg tgccggtcag cccgtcgaag gcgatcgtcg cctcgtcgat cgcgccgttc
     8941 gtcccgttcg cgccgttgac gaccgagttc gtcaggctga atcccgagac cccgatgccg
     9001 cgaatcgcga agttcgggaa gtcgttgagc tgcatcctcg agagggagac gttcgagacg
     9061 ttcgagaggt agacgccgct ccccgcgacg gccccgtccg ctccggcctg gttcctgatc
     9121 gtcccgccgc tcccggccgt cccggtcccg gcgaccgtga agctccccga cgtcctcgag
     9181 aggtagatcc cgttcggccc gccgttggcg ttgacggccg tcagggtcac ggcgaggggg
     9241 gtcgtcccgg ccgtcctcgt gatctcgacg gccgtggcgc ccgtggtcgt gatcgtgccg
     9301 ctcggaacgg tcagggtccc tgcgttctcc gtcgccagga ggcctcgctg gtttccgtcg
     9361 gggctcaccg tgagcgctcc gaacgtcacg ttcccggcgt tggaggcgag gttcacgccc
     9421 gtcccgcccg acagcgtcgt ggaggtcgtc ccgaggctga tcgtcccgcc ggccacggac
     9481 gtctggacgt cgatgccgat tcccgccgga ttcgtgaccg tcgtcgtgcc cgaggtgaac
     9541 gtcccgccga tcccaacgag gttgagcccc ttgccggtgc tgttcgagga ggtcaggctc
     9601 gtgaacgtga tccccgaggc gctcgcggag acgcccgtga ggtcgacggc cgcggcgttc
     9661 gtcgccgaca ccgtgacgct gttctggacg gtaatgccgg tgatcgcgcc ggcctggttg
     9721 tccgtgaagc ccacggctcc ggtcgtggcg atgtcgaggg agctgacgag gacggagctg
     9781 ccgccgagcg tgaccgttcc ggtgagcgtc ggcttgccgg cagcgccgat cgtgagaccc
     9841 gagccgccga gcgagaacgc cgcgccctgg ccccagagga cctggttcgc atccaggatg
     9901 atggcccccg tggtggtcgc cgcgcccgtg tggacgaaga cgacgtcgcc ggctccagac
     9961 gagacgtcgg cgttggccag gttgttgaag ggcgagttcg aggtcccgat cccgttcgct
    10021 ccggagctgt tgacgtacca gacctgcccg acgagcgtga tggtgacgct cgccgcggtc
    10081 tgggactcga cgccgttgga cgtgaccttg tacgtgaacg tcgccgaggt ctccgcgccc
    10141 gcgttcggcg tgtagcggaa ggcacccgcc gccccgagcg tcacggtccc gttcgccggc
    10201 tgcgtcaggc tcgtgacggt caggcccggc ccgaggtcgt tcgagaggat gttggtggcg
    10261 ctcgcgacga acggcgtcgc cggcgtcggc gtgtacccgc ctgcgtagta ctcggtgttc
    10321 cccgcgccgt ttgcataggc gtccgcgacg gcgaccggtc gggagacgag ggtgtaggcc
    10381 ttcgcgatgg tgcaggtcgt cgccgtgttc gtcgcggtga ccgtgaacga gaaggagccc
    10441 gtcccgaggg ctccgccggg ggtccccgtg agcgcgccgg tcgcggggtt cagcgagagc
    10501 ccttccggca gggagccggc ggtgacggcg tacgtgaagc cgcccgagcc gccgctcgcc
    10561 gtgatggcct ggctgtaggc cgtgccgaac gtggcgtccg gaaggacccc ggcggccgga
    10621 gtcatgtcga ggacgttcac ggtgtagctc tgcgagcccg cgcagccgtt gacgtcggtg
    10681 gcggtgatgc tgaaggagaa cgaaccggcc gccgagagcg gccccgagag gacgccggtc
    10741 gtcggattca gggtcgttcc cggcgggagg gtcccggtcg acgtgaagcc gaacggtgcc
    10801 gtccccgccg acggggtgat cgtggtcgtg taggtcgatc cgaccgtcgc gcaggtcggg
    10861 acggcggcga ggccgatgac cgggcagatc cggaccgtga acgcgcggct cccggtgcag
    10921 ctcgtcgtgg tgtcggtcgc cgtgatcgtg aacgtgaact caccttcggc cgacggcgtt
    10981 cccgtgatcg cgccgttcgt gccgagcgtg aggccggtgg ggagagcccc ggccgtcacg
    11041 gcgaaggtat acgggccggt accgccggcc gccgtcgcgg tctccgggta ggccgttcct
    11101 acgatcccgc ccggaagcgt cgccgggctg accgtgatcg tcgggcagac gaccgtgacc
    11161 gagaaggggg ccgagccggt gcaggtcgcg ccggtcgccg agtcggtcgc cacgatcgtg
    11221 aagttgtacg tcccggccgc ggtcggggtc cccgaaagga cgcccgtcgt cgggttcagc
    11281 gtcaggccgg tcggcagcgt ccccgtggcg ctgaaggtcc acggcccgac gccgcccgtc
    11341 gccgtgaagg tgtgcgtgta gcccgccacg cccgccgtca cgttcgtcgg cggcgccccg
    11401 ccgccaaccg tgatggtcgc gcacgcgacg acgagcgtgt acgcctggtc tcccgtgcag
    11461 ttcgtcgggt cgtgcgtcgc tcggatcgtg acgttgaacg tccccaccgc cgtcggcgtg
    11521 cccgagatct cacccgtcgc cgggttcagc gttagccccg ccggcagggc gccggagagg
    11581 atcgagtacg agtagctgcc gcccgcgagc gcgctcgagg cggtgaccgt ctggctgtac
    11641 gccgtcgccg ccgtcgcgcc gggcagcgca ccgggcgaga gcgtgatcgt cgggcacgtg
    11701 atcgtcagcg ggtagctgcg cgagaccgcg catccgttcg cgtccgtcgc cgtcaccacg
    11761 accgtgaagc tccccggctc gcccggcgtg ccggaaagaa cgcccgtcgt cgggtcgagc
    11821 gccagccccg tcggcagccc cgtggcgctc cacgcgatcg tcgccccgct ccccgcctgc
    11881 gtgaaggtga ccggcccgta cgccgtcccc accgtccccg ccgcgaggct cgccggcgcg
    11941 ttgatcgcca tctccgggca gatccgcagc gtgaaggcca cgctccccgt gcagccgttc
    12001 ccgtccgtcg ccgtcacgac gaccgcgaag cccccggctt ccgtcggcgt ccccgtgagc
    12061 gctcccgtcg ccgcgtcgag cgccagcccc gccggcagtc ccgtcgcgct ccacgccacc
    12121 ggcaggacgc cccccgtctg cgcgaaggtg gtccccgcgt agacctggcc aatcgtcccc
    12181 tgcgccgccg tcgccggcgt cacctcgatc accgggcaga tccgcacctg gtacgccgtc
    12241 gccccgctgc acccgtactg gtccgtcgcc gtgatcgtga agctgaagag gtccgccgtc
    12301 gtcggcgtcc ccgtgatcgc tccgctcgct ccgtcgagcg aaaggcccgc cgggagcgtc
    12361 cccgccgtca ccgcgtagag gtacgcccct ccgccgccgg ccccgaggac ggtctggctg
    12421 tacgccgtcc cgaccgtgcc gttcacgagc gtccccggcc ccaccgtgat cgtcgcgcag
    12481 acgatgccga ccgtgtaggc ctggctcccc gtgcacccgt tcccgtccgt cgccgtcacc
    12541 gtgaagtcgt aggtccctgc cgccgtcggc gtccccgtga tcgctccgct cacgccgttg
    12601 agcacaaggc cgggcgggag ggctcccgtc gtcaccgcgt acgtcatggt cccgaggccg
    12661 cccgaagagc cgacgctctg gatataggca atgcccgtcg tgccgcccgg cagcgtcgcc
    12721 ggactcaccg tgatcaccgg gcactccacg accagcgtgt aggcccgcgt ccccgtgcac
    12781 ccgttcgcgt ccgtcgccgt caccgtgaag ctgaacgtcc ccgtcaccgt cggcgtcccc
    12841 gtgaggaccc ccgccgtcgt cagggtgacc cccgtcggca gcgccccgac cgtcacggcg
    12901 tacgtcaccg ccccgactcc gcccgtctgc gtcagcgcca ccggcccgta ggccgctccc
    12961 gccgtcgcgt tcgccaggct cgccgggctc accgcgatca ccgggcagtc gatcaccagc
    13021 gtgagcgccc gcgtccccgt gcacccgttc gcgtccgtcg ccgtgaccgt gaagctgaac
    13081 gtccccgtca ccgtcggcgt ccccgagaga acccccgccg tcgtcagggt gacccccgca
    13141 ggaagcgctc cggccgtgac cgcgtacgtc gccgccccga ctccgcccgc ctgcgtcagc
    13201 gtcaccggcc cgtacgccac tcccgccgtc ccgttcccga gcgtcgccgg cgagaccgtg
    13261 atgaccgggc actccaccac gagcgtgtag gcctgcgtcc ccgtgcactc gttcgcgtcc
    13321 gtcgccgtga ccgtgaaggg gaacgtcccc gtgacggtcg gcgtccccga gaggaccccg
    13381 gcgctcgaca gcgtcagacc cgccggcagc gctccagccg tcacggcata cgtcgccgtc
    13441 ccgacgccgc ccgcctgcgt cagcgtcacc gccccgtagg tcactcccgc cgtcccgttc
    13501 gagaggctcg ccgggctgac cgtgatcgtc ggacagctca cgacgagcga cgtcgcgagg
    13561 ctcccggtgc atccgaggga gtcggttgcg gtgaccgtga aggggaaggt cccgacgacg
    13621 gtcggcgtcc ccgagagaac gcccgccgcg ccgtcgaaag tcgttccggc cgggagcgca
    13681 cccgcggtga ccgcgaaggt cgtggcaccg accgcgccgg acacgctgaa gggcgtcggc
    13741 gcgatcgcca cgcccgccgt cgccgccggg agcgtcgccg tgacggaaat cgggttgcag
    13801 acgacgatcg gcctcggcac ggtcacgtcg cagccgttcg cgtcgcgata gacgatctcc
    13861 gagtcgtagc ggcccgcctc ggtcggcgtc ccctcgattc ggccgttgct cgaaaccgtg
    13921 agccccggcg ggaggcggcg cccctcgaag gtgcccgagc cggagccccc ggtgacgctc
    13981 aggacgacca tcgcgtaggg agtcccgacc gtgccgcccg gaaggtccgt cggcgaaatc
    14041 gacatcggcg ggcagccgac ctcgatcgtg taggcgcgtt cgccggcgca gccgttcgcg
    14101 tcagtgaccg cgacggagaa cgaggcgacg ccggcctctg ccggcgttcc ttcgagaaga
    14161 ccggccgcgg tgaggacgag gcccgcgggc aaggcacccg cggagatccg gaaggcgagc
    14221 ggcggccgcc cggccggagc cgtgaacgtg gctcccgcgt agggctcgcc gacggtcgcc
    14281 gcgacgagcg cggtcggctc gacgccgagg tctccgcaga ccgcgatctg gagcgtccgc
    14341 gtcttcacgc agctcccctt ccggaccgtc agggtcacgg cgacggggcc ggcctccggg
    14401 aaggaggtcg cgtacgagcg gacgtccttg tcgagctcga cctcggggcc cgcgccgatc
    14461 cggagagact gcgacgtcag ctccgcggtc ggcggatcga gcttgacggt ccaggagagc
    14521 gtcgtctcga cgcccggctt caccggcagt ggcgtcgcct cgaacgcgac gatagcgacc
    14581 ttcgtgcagt cgccgccccc cggaaggccg aacggacgcg tcacggcgtc tcccgagccg
    14641 ttgtcgatct ggctcacgta ggcgtcgagc gcgccctgct cgaccgttac gtcgatgcgc
    14701 gagttcggcg ggaccgtggc gagggagagc ccgtcgcgcg aggtcctgcc cgcgagcgac
    14761 gaggcgaacc agtcggcgac gccgagctgg ccccattcgc cgggagcgcg ccggaagggc
    14821 gtccgcccct gctcggcgcc gcgctcgtcc cggaggacga gagcccccgc cgcgccctcg
    14881 ggaccgccga cgagcgcgag gttcgtccgg gtgccgggga cgttcagcgt ctggtcgatg
    14941 cccgtgaaga agccggtagc gcccgcggcg aggagccccg tcggccagac cgagacgaac
    15001 tgctgcgccg agaacgtccc cttccggacg ccggccgggt cgacgttgtt cgtccgcgcc
    15061 gccacgagga ggggcgcctc cgcggaaacg aggagggcgc tggccgtcct ctcggggaag
    15121 ccgaggagcg cgaggaccct cggcacctcg acgacgccgt gcgccgggat cgggacgacc
    15181 gcgctcgccg ccgtgggcgc gccgagggat cggatcgtcg cgacgacggg ctccgcgccc
    15241 gggttgaaga tgcgcaggtc ggtgacccag aaggccccga ggaggcccgg cgagagggcg
    15301 gcgcccgaca cgaggcgctc ggtcgcgccc gacgggaccc gctcgcacgg aagggcgatc
    15361 gcgtcggagg tgacgttgtc gttgacgacg gcgtaggccg tcgcgtggcc ggccttcacc
    15421 tcgaattccg cgcgcccgac ggggagcgag gattccccgg cgatcgccgt caccggcttc
    15481 tgccagacct ggggagtctg ggccgagagg gtcgtctcgc ccgccaggct cccgtcggag
    15541 tcgaagacgc ggaccacgac gatcgttccc gggtcgacga gggtcacgga gaggttcgtc
    15601 cggtatcccg tcttcgggtc ggccgaatgg ctcacccaga tcgagtgccc cgtctcgccg
    15661 gcgccgagca ggtccgcctc gggcacggcg aagaggccga ggccgtacgg cccttccgcc
    15721 gcggcgacat tgcgtgtcgt gaggctcgcg aggagcgcgg catccgagag gaccgtgatc
    15781 gtccccgccc cgtcgtcggc gaggccgaag agcgtcttca gcgcggcttc gatccgcacg
    15841 ctctctcccg ccgcgagcgt ccgcgtgacg ggagccggag gcgtcttccc ggccatcgga
    15901 acgagcccga tcgtcgccgt cgccgcctcc tcgcccgggt tcgtcagctc gagggtcgaa
    15961 gagaagcgcg cgccctggag gccggcgagc tcggccgcgc ccggaaggta ggtcgtctcc
    16021 gccacgagga gcgtcgaagt cccgagagcg gcagcgagaa cgagcccgag ggcggtgcgg
    16081 tatgtcattt tcttgacctc acgagatcga cacgagtctt cgaatcgggg acgggccggt
    16141 cgtgcagatg gggctccggc gaagtcaccg gaactccggg gtcagttcag tgagatggag
    16201 aggtaaccgg aattcaacga gagacgagcc ggacgctacc acggttagac ccaaatgtgg
    16261 atcggatctc aaagtaaccc tttacaggtc cggattgaaa ccttcgcgcg cacgggtcgt
    16321 agccgtgatg cgtgtcgcac cggcgttgcg caggaactgt gactcgacgt cacacttttc
    16381 gaggaggact tgccccatgc ccgaaaatcc cggcatgtcg agacgaaagc tgatcggcct
    16441 gggcgcggcg acggtcgccg ccaccgccct gcccgacgcc ctcttccgcc tctcttccgc
    16501 cgcggccgcg accggcccga cggaggggat cggctacttc gcccgcttcg gcgtcacgga
    16561 gaagatgctc cgcgacggcc tctccgccgc gctctcgcgc ggggccgact acgccgacct
    16621 cttcttccag caccgcgtcg gcaacagcct cgcgctcgag gacggcgagg tgaaccgcgc
    16681 ctacgcgagc gtcgagctcg gcgtcggcgt gcgagccgtc aagggcgacc agacgggcta
    16741 cgcctacacg gaggacctca cccccgaagc gctcgttcag gccgcgaaga ccgcggccgc
    16801 gatcgccgac gggccggcga agccggggcc gaaggggctg aaggccgaca cttccctccc
    16861 ctcgcgctat cccgtgaaga cccggtggga ggacgtccgc ccgcagcaga agctgccgct
    16921 cctctccggc ctgaacgaga aggtcttcgc cgccgacaag cggatcacga aggtgaacgt
    16981 cttcttcggc gacgaggcgg gggcaatcct gatcgccgac tcgaccggcc gcgtcgtcga
    17041 ggacctccag ccgatgacgc gcctcggact ctcctgcgtc gccgagcagg acggcaagaa
    17101 ggagacgaac ggctacaacg tcgccggacg cgccgacctc ggctactact cccccgaccg
    17161 cctcgagcgg atcgtgaagg aggccgtctc ccgcaccgtc atcctcttcg aggcggtcca
    17221 gcctccggcc ggcgagatgc ccgtcgtcct gggcgccggc gcgtcgggca tcctcctcca
    17281 cgaggcgatc ggccacggga tggaggccga cttcaaccgg aaggggatct cgatcttcgc
    17341 cgaccggatc ggcaagccgg tcgcgaacaa gttcgtgaac atcgtcgacg aggggacgat
    17401 cgccggcgcc cgcggcgcga tcaacgtcga cgacgagggg aaccccgtcg gcaagacgat
    17461 gctcgtcgag aacggcatcc tgacgaccta cctccacgac tcgatctcgg cgaagcacta
    17521 caaggtgaag ccgacgggga acggccgccg cgagagctac gcctacgcgc ccgtcccccg
    17581 gatgcgcgcc acgtacatgc tccccggccc ccacaagaag gacgagatcg tcgcctcggt
    17641 gaagaagggg atctactgca cgaacttcac gaacgggcag gtgcagatcg gcgccggcga
    17701 cttcacgttc tacgtgaaga acggctacct catcgaggac ggcaagctga cgaccccgat
    17761 caaggacgtg aacatcatcg ggaacgg
//
LOCUS       DAOTEZ010000025        17779 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2131_length_17779_cov_5.931223, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000025 DAOTEZ010000000
VERSION     DAOTEZ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17779
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2131_length_17779_cov_5.931223"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="P5164_02300"
     CDS             complement(<1..371)
                     /locus_tag="P5164_02300"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRY42753.1"
                     /translation="MGDLTASLGARDRYERLLSAVRRLVPYDAACLLRLQGDDLVPLA
                     GHGLVAAALERPYPRSRHPRLEAILRSEAPLLFPHDSPLPDPFDGLLLADPHALDRIH
                     ACLGCPLTADGEVVGALTADAL"
     gene            539..1216
                     /gene="ric"
                     /locus_tag="P5164_02305"
     CDS             539..1216
                     /gene="ric"
                     /locus_tag="P5164_02305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HRY42754.1"
                     /translation="MNLPLQPESTLADLAVAYPAASRVFRRHGLDYCCHGRRPVSEAC
                     AEKGIDPAAVLTEVAAEARTAGDVETMADQPIPAIVERIIGWYHARLRTELPLLVQMA
                     RKVEGVHAEKESVPRGLAEHVEGLSRELIDHLDKEEQGLFPMLLSGRGGQAAAQVQSL
                     EREHEDVALALQKTRALTTDLVPPAEACTTWRALYLRLDELEAELMEHVHLENNVLFR
                     RALCAGM"
     gene            1231..3534
                     /locus_tag="P5164_02310"
     CDS             1231..3534
                     /locus_tag="P5164_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HRY42755.1"
                     /translation="MSTNLPGNDNRRWALLGGVLLFTFLVLGFFGREVYRQAPPIPER
                     VVTPDGATLMTKADILTGQQVWQSTGGQQLGSIWGHGAYQAPDWTADHLHREATALLD
                     VMAEKEGAQGFASLSPDRQAALKAALVREMRANTHDAATGVVTISAERAEAYRRAAAH
                     FDALFGGARELKPLREDYAMSETTVPDAARRKALTAFFFWTSWAATTERPGQAITYTN
                     NWPHEPLVANVPSGANVVWSLVSIALLLAGIGAVVWWTAFREKPEPAVTAPPVDPLVG
                     ITLTPSMRAVGKYVVAVLGLFALQVGLGALLAHYTVEGQALYGIPVGEWLPYALARTW
                     HVQTAVFWIATAFLAAGLFLAPVIGGHEPKFQRLGVNVLFGALLLVVVGSLAGEYLAI
                     HQVLPLDLSFWLGHQGYEYVDLGRVWQIALFVGLVLWLVLMLRAILPAARRRDDARSL
                     VFMFTGATTAIGLLYGAGFFFTAKTHLSVMEYWRWWVVHLWVEGFFEVFATAAIALLF
                     AKLGLVDKKHAGAAVIASSALFLFAGIPGTFHHLYFSGTPVSILAVGASFSALEVVPL
                     TLIGLEAFRTYKLQHATPWMAKYRWPIVFFVGVAFWNLVGAGVFGFLINPPLALYYMQ
                     GLNTTPVHAHTALFGVYGLLSLGLVLIIARKLSGVRAWKETQLKWAFWGMNVGLGLMV
                     ALSLLPIGLAQTWASVEHGLWYARSADFLQLPALQALRWLRILGDTVFLGGVAFLLWF
                     FVGLLTGWSYEKAEEPGFDDDVLPENA"
     gene            3647..4288
                     /locus_tag="P5164_02315"
     CDS             3647..4288
                     /locus_tag="P5164_02315"
                     /inference="COORDINATES: protein motif:HMM:NF039786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HRY42756.1"
                     /translation="MKRTTLPTFAAAALAAALAAALLFPATPAAAHCDSVEGPVVVDA
                     KGALEKGDVAPVLKWISAAQEPAIREAFRKTLAVRKLGAEAKEVADTAFFETLVRLHR
                     ETEGAAYTGLKSGVKEPAFIGQLESALTSGSVDAFSKLVGEHAAAGIQGRFSKALAAK
                     KTADASPADGRAYVAAYVELMHYTKAIVEAVHAEHAGPAAHAAPAKGAGHTCD"
     gene            complement(4314..7340)
                     /locus_tag="P5164_02320"
     CDS             complement(4314..7340)
                     /locus_tag="P5164_02320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRY42757.1"
                     /translation="MALPPARWASLAAAFALLLAHPRALSLSPERQLSQFAISTWQSA
                     DGLPMNSVLAMAQAADGSLWLGTEEGLVRFDGAEFEVRDRGNTPSMDAHNVQALCADE
                     DGNVWAGTYGAGLLRWDGRRFSRPAMDPEPERSRPIRALARGPDGSIWIGTAGGLECL
                     VDGRRTRFAADPGLASAAVLAISPGRKGRLWVATVAWLGVVENGALRRFVAEPAPEGI
                     ALSLAEGPDGALFVGTDQGLFRISPDLRTCERIGGLANEFVSALAVDRNGALWAGTNG
                     GLHRLGPDGRIERLTAADGLSTDMIQTLLLDRDGNLWFGTRGGGLGRLRDGDFVTYGG
                     AREGFVVDTVLASRSGGVWVGGWNGFLAHLRPDGRFEPLAARSALDGSSVRAFFEEAD
                     GTLLVGAWNGLYRVRAGRAERVPDRGLIRNVRTVHVDRSGAIWVGLEAEGLVRLKGGT
                     ARRFTTADGLGGNEVRALLEDREGTLWVATYGGLSRRTGEGFSTLTTRDGLRSDLVRC
                     LSLDADGDLWIGTYGGGIARLRRGEIASWDTRDGLFTDVAYAITEDDAGDFWISSNRG
                     VYRVARADLEARAGGEGPKLRCRSFGIADGMASEECNGGDPAVARTADGRIYFPTVRG
                     VVAVDPARLLPAAPPRVRIDSVVAGANAIDHRETAEVPAGARRLELRWAALGFLPPLK
                     TEFRYRLDGFDDEWVEARTRRVATYTNLPPGSYTFRVVAGVDGSFGSSETRLPVRVGR
                     LWWQTWWAQAALAIALAGLLGGAYRWRTGSLARERRRLEEAVAARSAELAEANRALEE
                     AVVTDPLTKAKNRRFLEMALDSERELAVRSSESRGAGRNRDLILYLVDLDRFKSVNDR
                     HGHRTGDLVLVETARRLQGALRRSDFLVRWGGEEFLVVARGSERLQADVQARRLLEAV
                     GGSAYSLGELSLVQTCSIGWAPFPWLEERPRDATIEEVLGYADRALYEAKRSGRNGAV
                     GYVAGGGARRDGTGESFEERPGRVVEVVRTEGPRTG"
     gene            7413..8384
                     /locus_tag="P5164_02325"
     CDS             7413..8384
                     /locus_tag="P5164_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HRY42758.1"
                     /translation="MTHAVRIHTPGGPEALSWDEVRVGPPGPGEARVRHTAIGLNFID
                     TYHRSGLYPLPLPAVLGSEGAGVVTEVGEGVAEVKPGDRVAYAGPVGAYSEERLVAAH
                     RLVPLPDDVDDRVAAAAMLKGMTARYLLRKTHRVEEGETILLHSAAGGVGLIAAQWAR
                     SLGATVIGTVSTDEKAELARAAGCDHVVVTSREDLVQRVDEITGGAGVRVVYDSVGRE
                     TFEASLDCLAPLGLMVSFGNSSGPVPPFAPLLLSQKGSLFLTRPTLMAYTARREDLLE
                     TAADLFAVIRSGAVSVRVGATFPLREAADAHRELEARRTTGSTVLVP"
     gene            8441..9166
                     /locus_tag="P5164_02330"
     CDS             8441..9166
                     /locus_tag="P5164_02330"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRY42759.1"
                     /translation="MPEETPRPETTDASRRDFLKGAAAAAVAAGALGFPAILEGAAPH
                     ELAPLPWPDTALEPVISAKTLSFHWGKHHRGYVDALNGLVKGGPLEGKPLADVVRASA
                     ADTASRAVFNNAAQAWNHDFFWKSLRPKGGAAPSGALAERIEASFGSLEGLKKELVAA
                     GLGQFGSGWAWLVADGAALKVVKTANADTPLTTGVAPLLVLDVWEHAYYLDWQNRRKD
                     YLTAVVDAYLNWEFAASNLPKKA"
     gene            complement(9253..10998)
                     /locus_tag="P5164_02335"
     CDS             complement(9253..10998)
                     /locus_tag="P5164_02335"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HRY42760.1"
                     /translation="MDFSIKVDPSTWQRYISTPKKGEAVLTDSFLNKGTAFTAREREE
                     LDILGLLPPAIFTIEQQLVRVYESFCAKPTPLEKYIYLASLHDRNEVLYYRLVHERIE
                     EMLPIVYTPVVGEACQKFSHIFRRGRGLYIGIDQQDHIEKILRNYHSSEPSVIVVTDG
                     ERILGLGDQGAGGMGIPIGKLCLYTACAGVSPYSTLPITLDVGTDNEERLADPLYVGL
                     RHKRVRGAEYQAFVDKFVAAVKKVFPNVLLQWEDFLKDNAIFQLERFRDELLTFNDDI
                     QGTASVVVAGIFGALRLTGGKMSDHRLVFAGAGASAHGIAELFVSALVEEGIPVEEAR
                     KRIWTCDTKGLAVSDRPGLEGFKKEFARDRAELASWKVADPARISLEETIENAKPSIL
                     VGVSATPGTFTEAMVRKMAELNERPLIFPISNPTSKAECTAEQAVTWSDGRAIVATGS
                     PFAPVVHGGVTHRIGQCNNAFIFPGIGLGVCVSKARRVSDGMFLAAAQALATHVTEAD
                     IAQSAVYPQIATIRDCSHSVACAVIRRAVAEGLAEPEMLNGLEETVRRAMWFPEYLPI
                     RYEPAGLPARRHAHA"
     gene            11298..13589
                     /locus_tag="P5164_02340"
     CDS             11298..13589
                     /locus_tag="P5164_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRY42761.1"
                     /translation="MEPLFDARTLLAASAVVFAGLVLAVALAWRELRAIRGPDRLALG
                     FGLFFLGLVLLAFKGRIPALLPAVLANVLVTVAAAFILEGTKLFYGLVPSRRLTVGVG
                     LLATAAFAATMLLRDGENARAILSSAFLTALVGASAWTAWRHRAPRGAGAFDLVTAVA
                     LWACAGLFWARGVAVGAGFVEGDILAGGPLLALPPLLCTIFAVVWTTTLLATTSQRLT
                     RVVRTQNDLLASLLSVARAAGAGEGLDETLTMVLDAAKLATGATGASLLLLDEHRQFT
                     RGLFTEGDSTLVVGPAAAAEMLESGLAGWVVRNGSVALVEDVRQDRRWRRLEAQDATI
                     RSALSAPISAGASLSGVLTLVHPDAGHFGEEQRHLIESTAAQIALALRTAQIADARRR
                     ASRELEALNAVLDISARRHASEEIAEESVAAICRLSSFSRVFVALPTEAGFRRIGRTD
                     GPEELRSSDLDGELGLAWESGLVRRSGGGGASGACRSLVIPLRHLGNRLGVASFESSG
                     PEGLDEDACAFAEALAEAISLGLGKEILSRAREELSRMLVHDLRGPISGVLGALELLR
                     ENESLDGDGKRLLDLAERNARRQVALVDGILELSRLEEGSLPVRRTDVHLPAVVDEVI
                     GRALPAAQARGISLVSALPPDLPPVRADAGLVARIVENLLANAVKFSAPGAGSVRLEG
                     RVEGGSVILDVKDSGPGICEELRPRLFEKFAVGNLPGRGSGLGLPFCRLAVEAQGGRM
                     WLQHPGPAAVFSFSLPLASPPPS"
     gene            13619..14524
                     /locus_tag="P5164_02345"
     CDS             13619..14524
                     /locus_tag="P5164_02345"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HRY42762.1"
                     /translation="MRRAAAVLPFLFLHLACDRPPAADPDYVREIEAARAARHAELTT
                     EDGWLTIVAREPLAPGENTFGSDPSSKVALAGAGIPPRAGAFVVREDASVVLRAEPGA
                     PVTVNGAAPTDAPLVPEGDEKADVVTVGRVRLAYFRKDGAPYVRARDPESARLRGFPG
                     LSHFPIDPAYRVEGRWEPYDAPRNVEVPSSRGPARTALAPGLVRFTLGGRELSLEPTV
                     ESPDDATLFFVFADATSGSESYGGGRFLSVPRPAAGGDRVVLDFNLAETPPCSLTPFA
                     SCPMALPRNTLPIRVEAGEKAPRHE"
     gene            14593..15501
                     /locus_tag="P5164_02350"
     CDS             14593..15501
                     /locus_tag="P5164_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HRY42763.1"
                     /translation="MRRVLPLLLLAAVALAACRRPPAADPAYAAEIGTARAMRETRLA
                     SESGWLTLVALHWLTPGENPFGSDPALPLSLEAPGIPARAGAFVLGPDGAVRLAVDAG
                     APVTVDGAPPTGAPLATDRDGKPSVVEVGRVRLTVIDRGGKLAVRARDPESPRRRDFR
                     GIEHFPVDPALRVEGTFEPYDAPREIEVPSAQGPAQKSLAPGLVRFTIGGKERTLEPT
                     VESPDDDTLFFVFGDATNGTETYGAGRFLYATAPKGVPSKVVLDFNLAQNPPCAFTPY
                     ATCPLVMPRNVLPERVEAGEKVPAGH"
     gene            complement(15542..16537)
                     /locus_tag="P5164_02355"
     CDS             complement(15542..16537)
                     /locus_tag="P5164_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42764.1"
                     /translation="MRTPAPRTTPLGALAASLLFSAFAPAAAAAEGFTGTWALAMRAD
                     DVERVPSKGAWTGLGGTTGATTRPGGGGFDLPIEVMTDARLLAVEDDGLRLRVTYPSG
                     RVRTFVTDGSKRYVDDGDGPADVVARRREGTVTVSSEWVRGYRLRETWELRADPRRLV
                     VTGRLKGRESHEYVRTYEPAPDGAAAAIAPSPSPAPAPAVRVPWSPAVPASTPAPAPE
                     EPGGLPAKEPPPVDRLSECTLRPPARTPPGELNGLAKTPQETAAKAAIASIAPLRADG
                     VLSSDAEVFDGCLVWPFTLRLPALGGVQEVFVDAGDGRVVKSDFIRLGPRPAGAP"
     gene            16661..17605
                     /locus_tag="P5164_02360"
     CDS             16661..17605
                     /locus_tag="P5164_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010308757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRY42765.1"
                     /translation="MRKLGATELEVFPVCLGGNVFGWTASEAESFAVLDAYVAAGGNF
                     VDTADAYSAWAPGNAGGESEEILGRWMASRGNRERVVVATKVGKLAGLTGLSAGTIRK
                     AAEDSLRRLRIDRIDLYYAHADDPATPLEETLGAFDALVREGKVREIAASNYAAPRLA
                     EALAVSSRLGLTRYVALQPHYNLVHRAEYEGELQALCVREGLAVFPYYALASGFLAGK
                     YRPGAAVASARSGAAAKHLDGPGLRVLATLDSVAAERGTTVAAIALAWLLGRPGIVAP
                     IASARTPDQLAELLPAAALSLTAEERDRLEAASRPPGG"
BASE COUNT         2346 a         6859 c         6113 g         2461 t
ORIGIN      
        1 agggcgtccg ccgtcagcgc gccgaccacc tctccgtcgg ccgtgagcgg gcagccgagg
       61 caggcgtgga tccggtcgag cgcgtgcggg tcggcgagaa ggaggccgtc gaacgggtcg
      121 ggcagcggcg agtcgtgcgg gaagaggagc ggggcttccg atcggaggat cgcttcgagc
      181 cggggatgac gcgatcgcgg gtaggggcgc tcgagcgcgg ccgcgacgag cccgtgcccc
      241 gcgagcggaa cgaggtcgtc cccctggagg cgaaggaggc aggcggcgtc ataaggaacg
      301 aggcggcgga ccgccgagag gagccgctcg tatcggtcgc gcgctccgag cgaggccgtc
      361 agatcgccca cgatctcgag gagaacctcg aggggctcag gttgcgattt cgacgacagc
      421 gttgcgatcc gcacgacacc ccttgctgtc gcattcataa cacgaactcg taacccattg
      481 aattcaaatg gcgtgagctg cggcacgacg cgtgcgaagg ggatggcgga ggcaaccgat
      541 gaaccttccg cttcagcccg aatcgaccct cgccgacctc gccgtcgcct acccggccgc
      601 ctcccgcgtc ttccgccgcc acggcctcga ctactgctgt cacggccggc gcccggtctc
      661 cgaggcgtgc gccgagaagg gcatcgaccc tgccgccgtc ctgaccgagg tcgccgccga
      721 ggcccgcacc gccggcgacg tcgagaccat ggccgaccag ccgatcccgg cgatcgtcga
      781 gcggatcatc ggctggtacc acgcgcgcct ccgcaccgag ctccctctcc tcgtccagat
      841 ggcgcggaag gtcgagggcg tgcacgccga gaaggagagc gttccccgcg gcctcgccga
      901 gcacgtcgaa ggcctctcgc gggagctgat cgaccacctc gacaaggagg agcaggggct
      961 cttcccgatg ctcctctccg ggcgcggggg ccaggccgcg gcgcaggtcc agtccctcga
     1021 gcgcgagcac gaggacgtcg ccctcgccct ccagaagacc cgggcgctga cgacggacct
     1081 cgtcccgccg gccgaagcct gcacgacctg gcgagccctc tacctccgcc tcgacgagct
     1141 cgaggcggag ctgatggagc acgtccacct cgagaacaac gtcctcttcc gccgcgcgct
     1201 ctgcgcggga atgtgagtcg aaagggcacg atgagcacga accttcccgg caacgacaac
     1261 cgccgctggg cgctcctcgg cggagtcctc ctcttcacct tcctcgtcct cggattcttc
     1321 ggccgcgagg tctaccggca ggcccctccg atcccggagc gcgtcgtcac cccggacggc
     1381 gcgaccctca tgacgaaggc cgacatcctg accggccagc aggtctggca gtcgacgggc
     1441 ggccagcagc tcggctcgat ctgggggcac ggcgcctacc aggcccccga ctggacggcc
     1501 gaccacctcc accgcgaggc gacggccctc ctcgacgtga tggcggagaa ggagggggcg
     1561 cagggcttcg cctccctctc ccccgaccgc caggccgcgc tgaaggcggc cctcgtccgc
     1621 gagatgcggg cgaacacgca cgacgccgcg accggcgtcg tgacgatctc cgccgagcgg
     1681 gccgaggcgt accgtcgcgc ggcggcccac ttcgacgccc tcttcggcgg ggcgcgggag
     1741 ctgaagccgc ttcgcgagga ctacgccatg tcggagacga ccgtccccga cgcggcgcgc
     1801 cgcaaggcgc tcacggcctt cttcttctgg acgagctggg ccgcgacgac cgagcgcccc
     1861 ggccaggcga tcacctacac gaacaactgg ccccacgagc cgctcgtggc gaacgtcccg
     1921 tcgggcgcga acgtcgtctg gtcgctcgtc tcgatcgccc tcctcctcgc cgggatcggc
     1981 gccgtcgtct ggtggacggc gttccgcgag aagcccgagc ccgccgtcac cgcccccccg
     2041 gtcgacccgc tcgtcgggat cacgctgacg ccgtcgatgc gcgccgtcgg aaagtacgtc
     2101 gtcgccgtcc tcggcctctt cgccctccag gtcggcctcg gcgcgctcct ggcccactac
     2161 accgtcgagg ggcaggcgct ctacgggatc cccgtcggcg agtggctccc ctacgcgctc
     2221 gcgcggacct ggcacgtgca gaccgccgtc ttctggatcg cgaccgcgtt cctcgccgcc
     2281 ggcctcttcc tcgcgcccgt catcggcggg cacgagccga agttccagcg cctcggcgtg
     2341 aacgtcctgt tcggcgcgct cctcctcgtc gtcgtcggct cgctcgcggg cgagtacctc
     2401 gcgatccacc aggtcctccc gctcgacctc agcttctggc tcggccacca ggggtacgag
     2461 tacgtcgacc tcgggcgcgt ctggcagatc gccctcttcg tcggcctcgt cctctggctc
     2521 gtcctgatgc tccgggcgat cctcccggcc gcgcgccgcc gcgacgacgc ccgctccctc
     2581 gtcttcatgt tcaccggcgc gacgacggcc atcggcctcc tctacggcgc cggcttcttc
     2641 ttcaccgcca agacgcacct ctccgtcatg gagtactggc gctggtgggt cgtccacctc
     2701 tgggtcgagg gcttcttcga ggtcttcgcc acggcggcga tcgcgctcct cttcgcgaag
     2761 ctcggcctcg tcgacaagaa gcacgcgggc gcggcggtca tcgcctcctc ggcgctcttc
     2821 ctcttcgccg gcatccccgg caccttccac cacctctact tcagcggcac gccggtctcc
     2881 atcctcgcgg tcggggcgtc cttcagcgcc ctcgaggtcg tcccgctgac cctcatcggc
     2941 ctcgaggcgt tccggacgta caagctccag cacgccaccc cctggatggc gaagtaccgc
     3001 tggccgatcg tcttcttcgt cggcgtcgcg ttctggaacc tcgtcggcgc gggcgtcttc
     3061 ggcttcctca tcaacccgcc gctcgccctc tactacatgc aggggctcaa cacgacgccg
     3121 gtccacgccc acaccgccct cttcggcgtc tacgggctcc tctccctcgg cctcgtcctg
     3181 atcatcgccc gcaagctcag cggcgtccgc gcctggaagg agacgcagct gaagtgggcg
     3241 ttctggggga tgaacgtcgg cctcggcctg atggtcgccc tctcgctcct cccgatcggc
     3301 ctcgcgcaga cctgggcgag cgtcgagcac ggcctctggt acgcccggag cgccgacttc
     3361 ctccagctcc ccgccctcca ggcgctccgc tggctccgga tcctcggcga caccgtcttc
     3421 ctcggcgggg tcgcgttcct cctctggttc ttcgtcggcc tcctgaccgg ctggagctac
     3481 gagaaggcgg aggagccggg cttcgacgac gacgtcctcc cggagaacgc gtaggccgtc
     3541 ctcgtcccgc gccttccgga accggcccgc gtcgcgctcc cgcgcggcgc gggcgttccc
     3601 ccgaaacccc gttcaaccgg gccgaacggc ccgaaggagt gaagagatga aacggaccac
     3661 gctcccgacc ttcgccgccg cggccctcgc cgcggccctc gccgcggccc tcctcttccc
     3721 cgccactccc gccgcggcgc actgcgactc cgtcgagggc ccggtcgtcg tcgacgcgaa
     3781 gggcgcgctc gagaagggcg acgtcgcgcc cgtcctgaag tggatctcgg cggcgcagga
     3841 gcccgcgatc cgcgaggcgt tccggaagac cctcgccgtg aggaagctcg gcgccgaagc
     3901 gaaggaggtc gccgacaccg ccttcttcga gacgctcgtc cgcctccacc gcgagaccga
     3961 gggagccgcc tacaccggcc tgaagtcggg cgtgaaggag cccgccttca tcgggcagct
     4021 ggagtcggcg ctcacctccg gctcggtcga cgccttctcg aagctcgtcg gcgagcacgc
     4081 ggcggccggc atccagggac ggttctcgaa ggccctcgcg gcgaagaaga cggccgacgc
     4141 gtcgccggcc gacggccgcg cctacgtcgc cgcctacgtc gagctgatgc actacacgaa
     4201 ggcgatcgtc gaggccgtcc acgcggagca cgccggcccg gccgctcacg ccgctcccgc
     4261 caagggcgcc ggccacacct gcgactgacg acgaacccgc cgcccgggcg ctctcagccg
     4321 gtccgaggac cctccgtccg gaccacctcg acgacgcgcc cgggccgctc ctcgaacgac
     4381 tcgccggtcc cgtcccggcg ggcgccgccc ccggcgacgt agccgaccgc gccgttccgg
     4441 cccgatctct tcgcttcgta gagggccctg tcggcgtacc cgagcacctc ctcgatcgtc
     4501 gcgtcgcgcg gccgctcctc gagccagggg aacggcgccc agccgatcga gcacgtctgc
     4561 acgagggaaa gctcgccgag cgagtacgcg gacccgccca cggcctccag caggcgccga
     4621 gcctgcacgt ccgcctggag gcgctccgag ccgcgggcca cgacgaggaa ctcctcaccc
     4681 ccccatcgaa cgaggaagtc gctccggcgg agagccccct ggaggcgccg cgccgtctcg
     4741 acgaggacga gatcgcccgt ccggtgcccg tgcctgtcgt tcaccgactt gaacctgtcg
     4801 aggtccacga ggtagaggat caggtcgcgg ttcctgccgg cgccccggct ttccgacgac
     4861 cggacggcga gctctctctc ggagtcgagc gccatctcca ggaatcggcg gttcttcgcc
     4921 ttcgtcaggg ggtccgtgac gaccgcctct tcgagcgccc ggttcgcctc ggcgagctcg
     4981 gcgctccgcg cggcgacggc ctcttcgagc cgccgccgct cgcgggcgag gctccccgtt
     5041 ctccaccgat acgccccgcc gaggagtccg gccagggcga tcgcgagcgc cgcctgcgcc
     5101 caccaggtct gccaccagag ccgcccgacc cggacgggaa gccgcgtctc cgagctgccg
     5161 aagctcccgt cgacgccggc gacgacacgg aacgtgtagc tccccggcgg gaggttcgtg
     5221 taggtcgcga cgcgccgcgt ccgggcctcg acccactcgt cgtcgaagcc gtcgaggcgg
     5281 tagcggaact ctgtcttgag cggcgggagg aacccgagcg cggcccagcg cagctcgagg
     5341 cgccgcgcac cggccgggac ctcggccgtc tcccggtggt cgatcgcgtt cgcgccggcc
     5401 acgaccgagt cgatccgcac gcgcggaggc gccgcgggaa ggagccgggc cggatcgacc
     5461 gcgacgacgc cccgaaccgt cgggaagtag atccggccgt cggccgtccg cgccaccgcc
     5521 gggtcgccgc cgttgcattc ctcgctcgcc atcccgtccg cgatcccgaa cgaccggcag
     5581 cgcagcttcg ggccctcgcc ccccgcgcgc gcctcgaggt cggcgcgcgc gacgcgatag
     5641 accccgcggt tgctgctgat ccagaaatcg cctgcgtcgt cctccgtgat cgcgtacgcc
     5701 acgtcggtga agagaccgtc ccgggtgtcc caggacgcga tctcgccccg gcggagccgc
     5761 gcgatgcccc cgccgtaggt cccgatccag aggtctccgt cagcgtcgag ggagaggcac
     5821 cggacgaggt cgctccggag gccgtcccgc gtcgtgagag tcgagaatcc ctcgccggtc
     5881 cggcggctca ggccgccgta cgtcgcgacc cagagcgtcc cctcgcgatc ttcgaggagg
     5941 gcgcggacct cgttcccccc gagtccgtcc gccgtcgtga agcgcctcgc cgtccctcct
     6001 ttcaggcgga cgagcccttc cgcctcgagg ccgacccaga tcgctccgct ccggtcgacg
     6061 tggaccgtcc tcacgttccg gatcaggccc cggtccggaa cgcgctccgc gcggccggcg
     6121 cgcacccggt agagcccgtt ccacgcgccg acgagcagcg tcccgtcggc ctcctcgaag
     6181 aaggcccgga ccgagctccc gtccagcgcc gaccgggccg cgagcggctc gaatcggccg
     6241 tccggtcgaa ggtgggcgag gaagccgttc cagccgccca cccacacgcc gccggagcgc
     6301 gaggcgagga ccgtgtcgac gacgaagccc tcccgcgctc ctccgtaggt gacgaagtcc
     6361 ccgtcgcgca ggcggccgag gcctccgccc ctcgtcccga accagaggtt gccgtcgcgg
     6421 tcgaggagga gcgtctggat catgtcggtc gagaggccgt ccgccgccgt cagccgctcg
     6481 atccggccgt ccggtccgag acggtggagc ccgccgttcg tcccggccca gagcgccccg
     6541 ttccggtcca cggcgagcgc ggagacgaac tcgttcgcca gccctccgat ccgctcgcag
     6601 gtgcggaggt cgggggagat ccggaagagc ccctggtccg tcccgacgaa cagcgcgccg
     6661 tccggtccct cggcaaggga gagggcgatc ccttccggcg cgggctcggc gacgaagcgc
     6721 cgcagcgctc cgttctccac gacgccgagc cacgcgaccg tcgccaccca gaggcgtccc
     6781 tttcgaccgg gcgagatcgc gagaacggcc gccgaggcga gccccgggtc cgccgcgaac
     6841 cgcgtccgcc ggccgtcgac gaggcactcg aggccgcccg cggtcccgat ccagatcgag
     6901 ccgtcgggcc cccgcgcgag cgcccggatc ggccggctcc gctcgggctc cggatccatg
     6961 gccggccgcg agaaccttcg gccgtcccac cgaaggagtc ccgcgccgta ggtcccggcc
     7021 cagacgttcc cgtcctcgtc cgcgcagagg gcctgcacgt tgtgggcgtc catcgacggc
     7081 gtgttgcccc ggtcgcgcac ctcgaactcg gccccgtcga agcggacgag cccctcctcg
     7141 gtcccgagcc agagcgaccc gtcggcggcc tgcgccatcg cgaggacgga gttcatcggg
     7201 agaccgtcgg cggactgcca ggtcgagatc gcgaactggc tcagctgccg ctccggcgag
     7261 agcgagagcg cccgcgggtg agcgagcagg agcgcgaagg ctgcggccag cgacgcccat
     7321 cgggccggag ggagcgccat cgccgaaagt ctagcccggc cgatcgcccc gcgggtccac
     7381 ccgccgcgat acgctggcgc gcgaggtgcc gcatgacgca cgccgttcgc atccacacgc
     7441 cgggcggacc cgaggctctc tcctgggacg aggtccgggt cggacctccg ggcccgggcg
     7501 aggcccgtgt ccggcacacg gcgatcggcc tcaacttcat cgacacgtac caccgcagcg
     7561 gcctctaccc gctccccctc cccgcagtcc tcggctcgga gggggccggc gtcgtcaccg
     7621 aggtcggcga gggcgtcgcc gaggtgaagc cgggcgatcg cgtcgcctac gccggccccg
     7681 tcggcgcgta ctccgaggag cggctcgtcg ccgcccaccg cctcgtcccg cttcccgacg
     7741 acgtcgacga ccgcgtcgcc gccgccgcga tgctgaaggg gatgaccgcg cgctacctgc
     7801 tccggaagac gcaccgcgtc gaggaaggcg agacgatcct cctccactcg gccgccgggg
     7861 gggtcggcct catcgccgcg cagtgggccc ggagcctcgg cgccaccgtc atcggcaccg
     7921 tctcgaccga cgagaaggcc gagctcgcgc gcgcggcggg atgcgaccac gtcgtcgtca
     7981 cctcgcggga ggacctcgtg cagcgggtcg acgagatcac cgggggcgcc ggcgtgcgcg
     8041 tggtctacga ctccgtcgga cgggagacgt tcgaggcctc gctcgactgc ctcgcgccgc
     8101 tcggcctcat ggtctcgttc gggaactcct ccggccccgt gccgccgttc gccccccttc
     8161 tcctctcgca gaaggggtcg ctcttcctca cgcggccgac gctcatggcc tacaccgccc
     8221 gtcgcgagga cctcctcgag accgcggccg acctcttcgc cgtgatccgt tccggcgcgg
     8281 tgtccgtccg cgtcggcgcg acgttcccgc tccgggaggc cgccgacgcg catcgcgagc
     8341 tcgaggcgcg gcgcacgacc ggctccaccg tcctcgtccc ctgaggcggg gagagcgccg
     8401 ccgggtatcc tccgccccga cgaccaccag gaggtacacg atgcccgaag agacgccccg
     8461 tcccgaaacg accgacgcct cgcgccgcga cttcctgaag ggagccgccg cagccgccgt
     8521 cgccgccggc gcgctcggat tccccgcgat cctcgaaggg gccgcgccgc acgagctcgc
     8581 gccgctcccc tggcccgaca cggccctcga gccggtcatc tccgcgaaga cgctctcgtt
     8641 ccactggggc aagcaccacc gcgggtacgt cgacgccctc aacgggctcg tcaagggagg
     8701 cccgctcgaa ggcaagccgc tcgccgacgt cgtccgcgcc tcggcggccg acacggcctc
     8761 gcgcgcggtc ttcaacaacg cggcccaggc gtggaaccac gacttcttct ggaagagcct
     8821 ccggccgaag ggcggcgccg ccccgtcggg ggcgctcgcc gagaggatcg aggcctcctt
     8881 cgggagcctc gagggcctga agaaggagct cgtcgccgcc ggcctcggcc agttcgggag
     8941 cggctgggcc tggctcgtcg ccgacggcgc cgcgctgaag gtcgtcaaga cggcgaacgc
     9001 cgacacgccc ctgacgaccg gtgtggcgcc gctcctcgtc ctcgacgtct gggagcacgc
     9061 ctactacctc gactggcaga accgccggaa ggactacctc accgcggtcg tcgacgccta
     9121 cctgaactgg gagttcgcgg cctcgaacct gccgaagaag gcctgagacg cgtcggcccg
     9181 gctccggccg ggccgacggt aacggtcttc gaccgggagc gcgccgcacg gcgcggcgcg
     9241 ctcccggcgc aatcaggcgt gcgcgtgccg gcgggcggga agccccgccg gctcgtagcg
     9301 gatcgggagg tactccggga accacatcgc gcgccggacg gtctcctcga gaccgttgag
     9361 catctccggc tcggcgagcc cttccgccac cgctctccgg atgaccgcgc aggcgaccga
     9421 gtgcgagcag tcgcggatcg tcgcgatctg ggggtagacg gcggactgag cgatgtcggc
     9481 ctcggtgacg tgagtcgcca gggcctgggc cgcggcgagg aacatgccgt cgctgacgcg
     9541 ccgggccttg gacacgcaga cgccgagccc gatccccggg aagatgaagg cgttgttgca
     9601 ctggccgatc cggtgcgtca cgccgccgtg gacgaccggc gcgaacgggc tccccgtcgc
     9661 cacgatggcc cggccgtcgg accaggtgac ggcctgctcg gccgtgcact cggccttcga
     9721 ggtcgggttc gagatcggga agatgagcgg ccgctcgttg agctcggcca tcttcctcac
     9781 catcgcctcg gtgaacgtcc ccggcgtcgc cgagactccg acgagaatcg acggcttcgc
     9841 gttctcgatc gtctcctcga gcgagatgcg ggccgggtcc gcgaccttcc aggaggcgag
     9901 ctcggcgcgg tcgcgcgcga actccttctt gaacccctcg aggcccgggc ggtcggagac
     9961 ggccagcccc ttcgtgtcgc aggtccagat ccgcttccgc gcctcctcca ccgggatgcc
    10021 ttcctcgacg agcgccgaga cgaagagctc cgcgatgccg tgggcggagg cgccggcccc
    10081 ggcgaagacc agccggtggt cgctcatctt cccgcccgtc aggcgaagcg cgccgaagat
    10141 ccccgcgacg acgaccgaag cggtcccctg gatgtcgtcg ttgaacgtca ggagctcgtc
    10201 ccggaagcgt tcgagctgga agatcgcgtt gtccttcagg aagtcctccc actggaggag
    10261 gacgttcggg aagaccttct tcaccgcggc gacgaacttg tcgacgaagg cctggtactc
    10321 cgcgccgcgg acgcgcttgt ggcgaagccc gacgtagagc gggtcggcga ggcgctcctc
    10381 gttgtcggtg ccgacgtcga gggtgatcgg gagggtcgag tagggcgaga ccccggcgca
    10441 ggccgtgtag aggcagagct tcccgatcgg gatgcccatg ccgcccgccc cctggtcgcc
    10501 gaggccgagg atccgctcgc cgtcggtcac gacgatgacg gacggctcgg acgagtggta
    10561 gttccggagg atcttctcga tgtggtcctg ctggtcgatg ccgatgtaga ggccgcgccc
    10621 gcggcggaag atgtgggaga acttctggca cgcctcgccg acgacgggcg tgtagacgat
    10681 cggcagcatc tcctcgatgc gctcgtggac gaggcggtag tagaggacct cgttccggtc
    10741 gtggaggctc gcgaggtaga tgtacttctc gagcggcgtc ggcttcgcgc agaacgactc
    10801 gtagacccgg acgagctgct gctcgatcgt gaagatcgcc ggcggaagga ggccgaggat
    10861 gtcgagctcc tcccgctctc tcgccgtgaa ggccgtcccc ttgttgagga acgagtccgt
    10921 caggaccgct tcgcccttct tcggcgtcga gatgtatcgc tgccacgtgg acgggtcgac
    10981 cttgatcgaa aagtccatgc atcctcctcg cggccccggc ccgacatccc cactgcgagc
    11041 gccgcttccg ggacgccacg cctcgcgacg acctcgttcc gggaccgtcc tccagaatac
    11101 aggatcgggg cttccccgga ggcgtcgaaa tcgcgaccgc gaccgcccgg gcccgcctct
    11161 gccgcggggg ggcgggccgg tccggctcgg gtcccgacac acgcccggaa tcgtgctccc
    11221 gtcgaacgcc cggtccgccc gaacgccgtt cgacgggagg acgatgtccc ctccggctcg
    11281 tatgatgtga gaccgcgatg gagcccctct tcgacgcgcg gacgcttctc gcggcgagcg
    11341 ccgtcgtctt cgcgggcctc gtgctcgcgg tcgcgctcgc gtggcgggag ctccgcgcga
    11401 tccgggggcc ggaccggctc gcgctcgggt tcggcctctt cttcctcggg ctcgtcctcc
    11461 tcgcgttcaa ggggaggatt cccgcgctcc tgccggccgt cctcgcgaac gtcctcgtga
    11521 cggtcgccgc cgccttcatc ctcgagggga cgaagctctt ctacggcctc gtcccgagcc
    11581 gccgcctgac cgtcggcgtc ggcctcctcg cgaccgccgc gttcgccgcc acgatgctcc
    11641 tccgcgacgg cgagaacgcc cgggcgatcc tgagcagcgc gttcctcacc gcgctcgtcg
    11701 gcgcctccgc ctggaccgcc tggcgccacc gggcgccccg gggagccggc gccttcgacc
    11761 tcgtcaccgc cgtcgcgctc tgggcctgcg ccggcctctt ctgggcgcgc ggcgtcgccg
    11821 tcggcgccgg cttcgtcgag ggcgacatcc tcgcgggggg gccgcttctc gccctccctc
    11881 cgctcctctg cacgatcttc gccgtcgtct ggacgacgac ccttctcgcc acgaccagcc
    11941 agcgcctgac ccgggtcgtg cggacccaga acgacctcct cgcgagcctc ctctccgtcg
    12001 cgcgggccgc gggcgccggg gaggggctcg acgagacgct caccatggtc ctcgacgccg
    12061 cgaagctcgc gaccggcgcg acgggcgcga gcctcctcct cctcgacgag caccgccagt
    12121 tcacccgcgg ccttttcacc gagggggact ccacactcgt cgtcgggccg gccgcggcgg
    12181 ccgagatgct cgagagcggc ctcgccggat gggtcgtgag gaacgggtcg gtggcgctcg
    12241 tcgaagacgt ccggcaggac cgccgctggc gccgcctcga ggcccaggac gcgacgatcc
    12301 ggtccgcgct ctcggcgccg atctccgccg gcgcgtccct ctccggggtc ctgacgctcg
    12361 tccatcccga cgccgggcac ttcggggagg agcagcggca cctcatcgag tcgacggcgg
    12421 cgcagatcgc gctcgccctc cggaccgcgc agatcgccga cgcgcggcga cgcgcctctc
    12481 gcgagctgga ggcgctcaac gccgtcctcg acatctcggc ccggcggcac gcctcggagg
    12541 agatcgccga ggagtccgtc gcggcgatct gtcggctctc gtccttctcc cgggtcttcg
    12601 tcgccctccc gacggaagcc ggcttccgcc ggatcggccg gaccgatggc ccggaagagc
    12661 tccggtcgtc ggacctcgac ggcgagctcg gcctcgcctg ggagagcggc ctcgtccggc
    12721 gatcgggagg aggcggcgcg agcggggcct gccggagcct cgtcatcccg ctccgtcacc
    12781 tcggcaaccg gctcggcgtc gcgtcgttcg agtcctccgg cccggagggc ctcgacgagg
    12841 acgcctgcgc gttcgcggag gccctggccg aggcgatcag cctcgggctc gggaaggaga
    12901 tcctctcccg cgcccgggag gagctctccc ggatgctggt ccacgacctc cgcggcccga
    12961 tctcgggcgt cctgggcgcc ctcgagctcc tccgcgagaa cgagtccctg gacggcgacg
    13021 ggaagcggct cctcgacctc gcggagagga acgcacgccg ccaggtggcc ctggtcgacg
    13081 gcatcctgga gctctcccgg ctggaagagg gctcgctccc cgttcgccgg acggacgtcc
    13141 acctcccggc ggtcgtcgac gaggtgatcg ggagggcgct cccggccgca caggcccgcg
    13201 gcatctcgct cgtctcggct ctcccgcccg acctgccgcc ggtgcgcgcc gacgccgggc
    13261 tcgtcgcgcg catcgtcgag aacctcctgg cgaacgcggt caagttcagc gcgcccggcg
    13321 ccggctccgt gcggctggag ggacgggtcg agggggggag cgtgatcctc gacgtgaagg
    13381 attcgggccc cggcatctgc gaggagcttc gaccccggct cttcgagaag ttcgccgtcg
    13441 ggaaccttcc cggccgcggt tccggcctcg gacttccgtt ctgtcgcctc gccgtcgagg
    13501 cgcagggagg ccggatgtgg cttcagcacc cgggcccggc ggccgtcttc tccttcagcc
    13561 tgccgctcgc gagccctccc ccgtcgtaac gtctcccggg gccgtgctag aaaggcgcat
    13621 gcgccgcgcg gccgccgtcc tcccgttcct ctttctccac ctcgcctgcg accgcccccc
    13681 cgccgccgac ccggactacg tgcgggagat cgaggcggcg cgcgccgccc ggcacgccga
    13741 gctcacgacg gaggacggct ggctgaccat cgtggcgcgc gagccgctcg cgccgggaga
    13801 gaacacgttc ggctccgacc cgtcctcgaa ggtcgccctc gcgggcgccg gcatcccgcc
    13861 ccgggcgggc gcgttcgtcg tccgggagga cgccagcgtc gtccttcggg cggagccggg
    13921 cgcgcctgtg acggtgaacg gagccgcgcc gaccgacgcg ccgctcgttc cggaggggga
    13981 cgagaaggcc gacgtcgtca ccgtgggccg tgtgcgcctg gcgtacttcc ggaaggacgg
    14041 cgcgccctac gtgcgcgccc gggaccccga gagcgctcgg ctccgcggct tccccggtct
    14101 ctcgcacttc ccgatcgacc ccgcgtaccg tgtcgaagga cgctgggagc cgtacgacgc
    14161 accccgcaac gtcgaggtgc cctcctcccg ggggccggcc cgaacggccc tcgcgcccgg
    14221 gctcgtccgg ttcaccctcg gcgggaggga gctctcgctc gagccgaccg tcgagtcgcc
    14281 cgacgacgcg acgctcttct tcgtcttcgc cgacgcgacc tccggctccg agagctacgg
    14341 cggcgggcgc ttcctctcgg tcccgcggcc ggccgccgga ggggatcgcg tcgtcctcga
    14401 cttcaacctc gccgagacgc cgccctgctc gctgacgccc ttcgcgagct gcccgatggc
    14461 gctcccgcgg aacactctgc cgatccgcgt cgaggccggg gagaaggctc cgagacacga
    14521 gtgaccgtcg ccggaggctc gcggcgcttc gccccgaggg acgcccttcc ggccgccgtg
    14581 ctagaagggg ccatgcgccg cgtccttccc ctcctcctcc tcgccgccgt cgccctcgcc
    14641 gcctgccggc gccctccggc cgcggacccc gcttacgcgg ccgagatcgg cacggcccga
    14701 gcgatgcgcg agacgcgcct cgcatccgag agcggatggc tcacgctcgt cgccctccac
    14761 tggctgactc cgggcgaaaa cccgttcggc tcggaccccg ccctcccgct ctcgctggaa
    14821 gcgcccggca ttccggcgag ggccggggcg ttcgttctcg gtcccgacgg cgccgtccgg
    14881 ctcgccgtgg atgccggcgc ccccgtcacc gtcgacggcg ccccgccgac cggcgcaccg
    14941 ctcgccaccg accgggacgg gaaaccctcc gtcgtcgagg tgggacgcgt ccgcctgacg
    15001 gtcatcgacc gaggcggcaa gctcgccgtc cgggcccggg accccgagag cccgcggcgg
    15061 agggatttcc ggggaatcga gcacttcccc gtcgacccgg cgctccgggt cgaggggacg
    15121 ttcgagccgt acgacgcgcc ccgcgagatc gaggtccctt ccgcccaggg tccggcccag
    15181 aagtctctcg cgccgggtct cgtccgcttc acgatcgggg ggaaggagcg gacgctcgag
    15241 ccgaccgtcg agtcgccgga cgacgacacg ctcttcttcg tcttcggcga cgcgacgaac
    15301 ggaacggaaa cctacggcgc cggacgcttc ctctacgcga ccgcgccgaa gggcgtccca
    15361 tcgaaggtcg tcctcgactt caacctcgcc cagaacccgc cctgcgcctt cacgccctac
    15421 gcgacctgcc cgctcgtgat gccgaggaac gtcctcccgg agcgcgtcga ggcgggcgag
    15481 aaggtgccgg ccgggcactg atcggcgcgc cgcctcgggc gcgcgccctg ggcggcttcc
    15541 gtcaaggagc tccggccggc ctcgggccga ggcgaatgaa gtccgacttc acgacgcgcc
    15601 cgtcgcccgc gtcgacgaag acctcctgga cgccgccgag cgccggcagg cggagggtga
    15661 acggccagac caggcagccg tcgaagactt ccgcgtcgga ggagaggacg ccgtccgcgc
    15721 gaagcggcgc gatggacgcg atggccgcct tcgcggcggt ctcctgcggt gtcttcgcga
    15781 gcccgttcag ctcgccgggc ggagtcctcg cgggcggacg gagagtgcat tccgagagac
    15841 ggtcgaccgg cggcggctcc ttcgcgggca agccgcccgg ctcctccggc gccggagcgg
    15901 gcgtcgatgc cggaacggcg ggcgaccagg ggacccggac ggccggagcc ggggccgggg
    15961 agggagacgg cgcgatcgcg gcggccgctc cgtcgggggc cggctcgtag gtccggacgt
    16021 attcgtgcga ttccctcccc ttcaggcgac cggtcacgac gagccggcgc gggtcggccc
    16081 gcagctccca cgtctcgcgc agccggtagc cgcgcaccca ctcggacgag accgtcaccg
    16141 tcccttcccg ccggcgcgcg acgacgtcgg ccgggccgtc gccgtcgtcg acgtagcgct
    16201 tgcttccgtc ggtcacgaac gtccggacgc gcccggacgg gtaggtgacc cggagccgga
    16261 ggccgtcgtc ctcgaccgcg aggaggcgag cgtccgtcat cacctcgatc ggcaggtcga
    16321 agccccctcc gcccgggcga gtcgtggccc cggtcgtccc gcccaggcct gtccacgccc
    16381 ccttcgaagg aactcgctcg acgtcgtcgg ccctcatcgc gagggcccag gtgcccgtga
    16441 acccctcagc cgcagccgcc gccggcgcga aggccgagaa gaggagcgac gccgcgaggg
    16501 cgccgagggg ggtcgttcga ggggcggggg tgcgcatcga gcctccgggg ccgagtccgg
    16561 cccgcaccga gagtagccgc tccggtcgct cggcggggcc cggacaccag cgggtcgcgc
    16621 catgagatcc tctcgcgtga gcatcgccca cggagggacc atgagaaagc tcggcgccac
    16681 ggagctggag gtcttccccg tctgcctcgg agggaacgtg ttcggctgga ccgcctcgga
    16741 ggccgagtcg ttcgccgtcc tcgacgccta cgtcgccgcg ggcggcaact tcgtcgacac
    16801 ggccgacgcc tactcggcct gggcgccggg caacgccggc ggcgagtcgg aggagatcct
    16861 cgggcgctgg atggcgtccc gcgggaaccg cgagcgcgtc gtcgtcgcga cgaaggtcgg
    16921 aaagctcgcg ggcctcaccg gcctctcggc ggggacgatc cggaaggccg ccgaggactc
    16981 gcttcggagg cttcggatcg accgaatcga cctctactac gctcacgccg acgacccggc
    17041 cacgccgctc gaggagacgc tcggagcctt cgacgccctc gtccgcgagg gaaaggtccg
    17101 ggagatcgcc gcttcgaact acgcggcacc ccgcctcgcc gaggcgctcg ccgtctcgag
    17161 ccggctcggg ctgacccgct acgtcgcgct ccagccgcac tacaacctcg tccaccgcgc
    17221 cgaatacgag ggagagctcc aggcgctctg cgtccgcgaa gggctcgccg tcttccccta
    17281 ctacgccctc gcgagcggat tcctcgccgg gaagtaccgc cccggagcgg ccgtggcgag
    17341 cgcgcgctcc ggcgccgccg cgaagcacct cgacgggccg ggtctccgcg tcctcgcgac
    17401 gctcgactcg gtcgccgcgg agcgcgggac caccgttgcg gccatcgcac tcgcgtggct
    17461 cctcgggcgg ccgggcatcg tcgccccgat cgcgagcgcc cggacgccgg accagctcgc
    17521 cgagctcctg cccgcagccg cgctctccct gaccgccgag gagagggatc ggctcgaggc
    17581 ggcgtcgagg ccgccgggcg gatgacgagc ctctgccgcg aagggctcgc tcgcgtcttc
    17641 gtctcaccgt ccgatggcgg cagacccatc tcggatggtt ggcctcccac ctgccgttcg
    17701 gtcgaaccgg agtaagatcg agccggcccg aaacgggggc aggcaccgga aacggagccc
    17761 cctcgcgccc ggcaaggca
//
LOCUS       DAOTEZ010000026        17723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2142_length_17723_cov_6.484945, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000026 DAOTEZ010000000
VERSION     DAOTEZ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17723
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2142_length_17723_cov_6.484945"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="P5164_02365"
     CDS             complement(<1..383)
                     /locus_tag="P5164_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase GatDE subunit E"
                     /protein_id="HRY42766.1"
                     /translation="MYPTLDYAEVGFVCGLEVHQQLLTPTKLFCRCPAGLYTETHDGT
                     VLRHMRPTLSELGEYDGTALMEFKTRKNIVYLLNVKNVCTYEMDDTPPFLVNQQAIDV
                     AIEQCLMLGCDIVDEVHIARKQYLDG"
     gene            complement(545..1267)
                     /locus_tag="P5164_02370"
     CDS             complement(545..1267)
                     /locus_tag="P5164_02370"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HRY42767.1"
                     /translation="MKKAVVLSVLLLASLSAAADEPPPGWHGSFGAGLSISSGNSDAK
                     SFNLSGDLKYDPKTKNVVKLGALFLRSESNGETTADKLVATIRDEYSVTDRFFVYGEV
                     GYLRDKVALLSYLISPIAGAGYKVVKTDALTFDVFGGIGGAFEKLEAHDATSSGAFRA
                     GEALAWKISPTVTLAQNGSGLWKANDTGDAFLHFDVSLATSLSKLLELKLAYLVDHKT
                     RPAFADLDKTDTAFLATVVAKF"
     gene            complement(1279..1695)
                     /gene="mscL"
                     /locus_tag="P5164_02375"
     CDS             complement(1279..1695)
                     /gene="mscL"
                     /locus_tag="P5164_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRY42768.1"
                     /translation="MLKEFKEFAIKGNVIDMAVGVVIGGAFGKIVNSLVADVIMPPIG
                     LLLGKVDFTNLFINLSGTPAASLAEAKEKGLATLNYGVFINTAFEFLIIAFAIFMVVK
                     QINRLKREAPPAPAAPPEPSAEEKLLGEIRDLLKSK"
     gene            1853..3616
                     /locus_tag="P5164_02380"
     CDS             1853..3616
                     /locus_tag="P5164_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020471837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRY42769.1"
                     /translation="MADDVGRVDRERRRLVALLAGAGAGAAVFGRALSALADGAAKVT
                     AGMVKQAEWIAGVTFTDAERELMLSDLNETAEGLAALRTVALDNAVPPAIAFSAGTAP
                     PADAGPAPAPSRRERPAVRPPSDDDLAFAGVRELGRLLRARKVSSTELTKLALARLRT
                     HGPALSCTVTVTEELALAQAKRADEELAAGKDRGPLHGIPWGAKDLIAVPGYPTTWGS
                     PLFREQVRPETATVYRRLEEAGAVLVAKTSVGELAWGDVWFGGTTKNPWKTDQGSSGS
                     SAGSASATAAGLVAFAIGTETWGSIVSPCTRCGVTGLRPTFGRVSRHGVMALSWTMDK
                     VGPIARSAEDGALVFAAIAGKDPLDPVSTDGPVPWPARRSVSELKVGFVEELFDEDRS
                     AGTENEEAKARAAEWRDFDRRSLGVLRELGVRLVPVKLPSRTPVAPLGMILTAEAAAA
                     FDTLVLDGRVKGMVRQTADAWPNVFRQGRLLPAADYLRAQRVRTLLMREMEECLRDVD
                     LLVAPTFGGDALLLTNLTGHPCVVVPNGFRLSDGTPTSLTFTGKLFGENDLLAVADLF
                     QRATDFHTRRPKLTEAAAAKG"
     gene            3677..6853
                     /locus_tag="P5164_02385"
     CDS             3677..6853
                     /locus_tag="P5164_02385"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRY42770.1"
                     /translation="MSLWSLPARGLFVAALLAGAGRASALDPATPLGQYGLDAWQEGL
                     PQNSVHALLQSRDGYLWLGTYEGLVRFNGVSFVVYDTRNTPALKSNSVWALHEDRDGT
                     LWAATLGGGVARVRGGVFARYGVRDGLPSEYVWALAEDSEGAVWAGTDAGIARFDGTS
                     FRAVTLPSPFERVPVRSLLGSPAGGLWIGTTTDGLLRWDGTTLRRIHPREGRAPGAVY
                     ALAPAPSGVAAASFGTGLVEATDDGRVTVRGRRDGLPNDLPWALLRDGTGALWVGTDG
                     GGVARLSGGRVETLTAADGLAHSFVRSIAEDREGSVWIGTNGGLNRLRDRKVVVYGAR
                     AGLPDENVRSMARTTDGTLWVGTDSGGISALRGSRFVVPPEAAPLRGVPVRALLAGTG
                     NDLWIGTNGGGLLRLSRGRLTRWTRKEGMPSDIVYALAIDRAGDVWAGTYGGGVARLF
                     GGRIEVIDRSRGLPRDVIRSIHHARDGVTWIGTDGGGLARLQDGSLSVLDSRHGLAND
                     TVFSFHEDAEGLLWIGTAGGLSVLRRDGRLRSVGLADGLPEERVFAILEGDGDGLFMS
                     SNKGIFRVSRADVLARMEGRRGPFPSLTLGRSDGMPIAQCNGASWPAALRDPDGKLWF
                     PTLRGLVGVDPARLPSNPLPPPVVVDRVLVDGRPVAGAPPVVLPPRSGKVEIHYDALS
                     LQAPEKVRFRYRLEGFETSWVDAGPRRSAFYTSLPPGDFVFRVVAANNDGVWNETGAR
                     FAFRLTPGLHQRTGFQVGVAAFLLAVVGTGYALRVRGLRSRQVELQRLVDERTRSLVE
                     EIQRAEELRRQAEAALREAAEANRAKSRFLANMSHELRTPLNAILGYAELLAEEPEMG
                     ASPGAREDVEKIRVAGGHLLQLIGDLLDLSKVEAGRMEVVAEPFAVREVVEETVRTVR
                     HLVEANRNALLVTIDDGVGEMTSDAFRLKQVLFNLLSNAGKFTEAGTVTLDVLRERRA
                     GGEVLVFRVADTGIGMSPEQLEKVFTPFVQGDASLTRRFGGTGLGLALSRHLARLMGG
                     GIWVTSAEGKGTAFVLELPAGTPEPHPA"
     gene            7006..7488
                     /locus_tag="P5164_02390"
     CDS             7006..7488
                     /locus_tag="P5164_02390"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HRY42771.1"
                     /translation="MKHSSRSNLVPVATALALALVAGPLLALDAPPDPPPGPQGPLAG
                     YLRCLGIVGLTDAQKADVKSLLEAAKPEMEAIREALKADRATLRTALAATPPDPCAVG
                     TALLEVEADVKAIREATKELRTAIEALLTPVQKAKLEGCLKAPRPNGPADEDGEGAEL
                     "
     gene            complement(7542..8549)
                     /locus_tag="P5164_02395"
     CDS             complement(7542..8549)
                     /locus_tag="P5164_02395"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HRY42772.1"
                     /translation="MTPSSPDGDRAEKGARLLLALADASPEVVLFATDPDGRVAWHGA
                     AALRELGLSPEAVLGRPLAELLAEGQGDSLPPFRSDEACRSGPRLLNLVAVDGSVVTV
                     RALLESGPDGLLVAGARLAAEEARLGEELLRLTNELATANREAVRRGRELERTLERLR
                     AANEVIEKLARTDPLTLLVNRRGLEEAIAHERDRAERTGEPLSVVALDLDRFKRVNDT
                     WGHAVGDRLLSAVGEALRGGVRPYDVAARMGGEEFLLVLPATRRERAAEVAERLRARV
                     AELVVEGLPERVTSSAGVAEHARGETTEALLERADAALYEAKRLGRDRVVLAPPPGPA
                     G"
     gene            complement(8546..9835)
                     /locus_tag="P5164_02400"
     CDS             complement(8546..9835)
                     /locus_tag="P5164_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42773.1"
                     /translation="METPTLPLSGRLREAVGPIHERIERLPFFAALSARTLPLERYVD
                     QLRAMAVVEATLERVLLSSRHPAVAAARDAVPSRLEALLSDLASFDRRGPLPDDPAPA
                     ATALELSRALLLLSAERPAALLGPLYVSTGMTLGNLVHLEDARACAGGGGTGWYEGHG
                     AGTGPAFRRFCSVLDELALASGEAASAVEAAVACAEGLERVHAVLDPATRPLRRFLAT
                     TLNPEAGAHPVPTDPSEMAAALRAGARCLEEHPYFLSRYGERGRRFTGSDAAWLVSLT
                     ALPPADVSGQVGWLASVLSRKGMPSLLLERQLVLLAEELRAGGVPRDAGVLEAAAREL
                     AGRRREALPEEVAARLAETFVASCGHGSGAEWRAAAGLLLAAAADEATGLAGTVRAVT
                     EWYRGPRHPATWNEAVDVLVRDALAASARREPEGPAG"
     gene            complement(9841..10491)
                     /locus_tag="P5164_02405"
     CDS             complement(9841..10491)
                     /locus_tag="P5164_02405"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRY42774.1"
                     /translation="MRSERERYLDAILAGRRQDAVEAALGALERGMDVRDVYVDVLQE
                     ALYEVGRLWQAGRISVATEHLATAITQLVVARLYEKLPRSETMRGRVVLTGVEGELHQ
                     VGGNMIADALEADGWDVRFLGTDVPGADVLDAIAAHRADLFGISCTLRQNVPKVSALV
                     DAVRSRFGESRPRILVGGGAFRASGGGSLDVGADAWAPDMREAIVVARSLEADGGR"
     gene            10694..11563
                     /locus_tag="P5164_02410"
     CDS             10694..11563
                     /locus_tag="P5164_02410"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRY42775.1"
                     /translation="MERESEGVFRGWRRPGPRPPGGLEPGEGETLDYLCGSFRIFQAA
                     RGHRYSTDDLLVAWWGVGNAPRVDRLLDLGSGIGSVAMAAAWKLPGARVVTVEAQAIS
                     IALARKSVRYNGLEERLRVVEGDFRDPALLEGEPPFDLVLGAPPYFERGTATEARHPQ
                     AVPARITLRGGIGAYAEAARRTLAPGGRFAFVWPVARDGEARDALRRESLLLLARRDV
                     VFREGEPPLVALYAAARAGDLPASLATAAGFPCVEPPLVVRRANGRHTPEYASVRLSM
                     GFPPGDLAPRPAA"
     gene            11619..13952
                     /locus_tag="P5164_02415"
     CDS             11619..13952
                     /locus_tag="P5164_02415"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY42776.1"
                     /translation="MVPDPPQALLPPLTTIPFGLGLDGRPIRHVNGKLVVGTIRHAID
                     LTLERELDALPAMPSPTERNAREAVVRARLLVRLVERLNGLIPDPKYHVTSETLLVDG
                     NNYSREFELYVNELCREFAGVEYFYFTRGGKVVPPTIAAIFRPFGVRQAYALLARAAE
                     KFVETDLRVVETTTRSAVVRWYAATQLEDVRDELQERWLLASCAAYQGTCAAIPALPY
                     PGQPWAEVDELKCQARGDECCEWRFTWEDPHPHAAHLLLAGAAASTGLLGWALSGSPG
                     SALAAPAILLPLLGAWHVARIRRLQHDRQEQDRLIAAQRATAESQVDRVQAAYAEVQQ
                     STLALRGKVAELSALREVALAVSASLDPFEVVDRSLEAVRRRLDYDRAVVLLVDETRN
                     VLSRARSRGLSPDQASLLARVSIRLDDPLSAIAPAVRDGVAFRLRDLPGPPGAATRQL
                     VPILGSEEVFVAPLLARGRSVGVLIVDNGTTRRPLPGEGLELLTAVASTIGVAVENAH
                     LYSQIEQQRRTLETRVRERTADLERATEEAESANRAKSAFLANMSHELRTPLNAIIGY
                     SEMLLDERAGADADGAASDLGKIRAAGLYLLGLINDVLDLSKVEAGKTELNLEPFSLS
                     ALVRDVASDVRPLVERGGNRFVVDAPSDETLHGDPRKVRQVLLNLLGNAAKFTEGGTV
                     ELRARRDGEEVLLSVADTGIGMTPDQVGRLFTAFWQGDPASARNYGGTGLGLAISRHY
                     ARLMGGDVSVVSEAGRGSTFTLRLPVRVAGVSSSRSA"
     gene            14011..16383
                     /gene="tssI"
                     /locus_tag="P5164_02420"
     CDS             14011..16383
                     /gene="tssI"
                     /locus_tag="P5164_02420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HRY42777.1"
                     /translation="MSDYTQSNNTFRITTPLGPDALLLRRIRGSEGISELFDLDLEVL
                     APNETEVPFDALLGQSVTVAAYGVDGSPVRHFNGICAELEAGPRETMEADKKTFTAYR
                     LRVVPKVWLLGLVHRSRVFQEMAVPAILKIVLDGIDASYRLERTYEPREMCLQYRESD
                     LAFACRLMEEEGIRFYFEHSDGSHLLVVTDRGGHEDLPGPSPLGYDPLEGGGRDEDRI
                     TSWHKRQSLTRGKVTLRDRSFELPTNDLEKVILLKDSLKAGTVTHKLRLAEADKRELY
                     AFPGGHAKPFDGVAPGGGERAGELNKTFPYGETRARVVMEREETRAIEIEGASNVRHL
                     VPGFQIKLDRHHDGNGSYLVTRVRHEGQNGSFRSDEVETRYENTFTCLPADAVYRPPR
                     LTRRPVAQGPETAIVVGPSGEEIYVDKYGRIKVQFYWHREGKRDTTSCAWLRVVTPLA
                     GAQWGSYAFPRIGQEVLVAFQHGDPDEPVVLGALYNAENMPPYTLPANRVRTGLKTRS
                     TLKGAADEFHEVRLDDTKDREEIFVQSQKDFHRLVKNNDLLQVGVKKGDKEPPDGSQK
                     QEIWKNRTVHVSKGDDFLNVGRLPGETQPAVDSRSGSDPGQSPSCGGNLTTWVANDRT
                     TTLNTGNDTLVVGLQAMGKSTPSLSVHQGNQVLEIQNDRTATVKYGNDSLTIRKGNLD
                     IKLEGSMPDGKVSIEAPNAIELKVGMNHVKIDTMGIELKFGTTQLKLDAMAAELKTTT
                     GSLKLDAASATLKGINATLQGNAIADLKAPIVNVRADGVASVGGAMTKIG"
     gene            16394..16999
                     /locus_tag="P5164_02425"
     CDS             16394..16999
                     /locus_tag="P5164_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42778.1"
                     /translation="MSSTGASPRTAAATILPRLDASAEAKGLLAPDAEASEYVALLVE
                     KELFSDATGFVAQWLPAHDAVAWACEAARTALGEAPPAERAALEAAERWLATRDEKER
                     RAAAEAAERLGHRSAPAMAAAAAGWTGGSLAPADLPAVAPPPGLAGKGASAAVLLAAA
                     AAAPEAPERLRALIWSGVDLAEGRRKPGLAGAARGRAGREG"
     gene            17003..17302
                     /locus_tag="P5164_02430"
     CDS             17003..17302
                     /locus_tag="P5164_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HRY42779.1"
                     /translation="MPQPAARVGDMHTCPMQTPAVPPVPHVGGPILPPGCPTVLIGGL
                     PAACVGDMAVCAGPPDVVVSGAFNVLVGGRPAARMGDPTAHGGVIVVGYPPVLVG"
     gene            17363..>17723
                     /locus_tag="P5164_02435"
     CDS             17363..>17723
                     /locus_tag="P5164_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HRY42780.1"
                     /translation="MPDGTSPPGTWDFVIVGSGFGGSVSALRLSEKGYRVLVLEKGPR
                     RSANDFAKTTWDLRRWLWMPGLGCRGIFRMTFLPHVTALSGVGVGGGSLVYANTLPVP
                     KRPFFEAPSWARLARWEE"
BASE COUNT         2414 a         6397 c         6609 g         2303 t
ORIGIN      
        1 ccgtcgaggt actgcttccg cgcgatgtgg acctcgtcga cgatgtcgca gccgagcatg
       61 aggcactgct cgatggcgac gtcgatcgcc tgctggttca cgaggaacgg cggcgtgtcg
      121 tccatctcgt acgtgcagac gttcttcacg ttgaggaggt agacgatgtt cttcctcgtc
      181 ttgaactcca tcagcgcggt gccgtcgtac tcgccgagct ccgagagggt cggcctcatg
      241 tgccggagga ccgtcccgtc gtgggtctcg gtgtagagcc ccgcggggca gcggcagaag
      301 agcttcgtcg gggtcaggag ctgctggtgg acctccagcc cgcagacgaa gcccacctcc
      361 gcgtagtcga gggtcgggta caaggacgcc cccttcccgc ccgggggcga cgggccccgg
      421 gcgaacgggg catcttagcg ccggagagga cggccggccc ggccggggac gccggggtcg
      481 gcggacggga cgaagccggc gccccgagac cgcggacgcg gtcccggggc gccgcggggc
      541 ggcgctagaa cttcgcgacg acggtcgcga ggaaggcggt gtcggtcttg tcgaggtccg
      601 cgaacgccgg ccgggtcttg tggtcgacga gatatgcgag cttcagctcg aggagcttcg
      661 agagcgaggt cgcgaggctg acgtcgaagt ggaggaaggc gtcgccggtg tcgttcgcct
      721 tccagaggcc cgagccgttc tgggcgagcg tcacggtagg ggagatcttc caggcaagag
      781 cctcgcccgc ccggaacgcg ccgctcgagg tggcgtcgtg cgcctccagc ttctcgaacg
      841 cgccgccgat gccgccgaag acgtcgaacg tcagggcgtc ggtcttcacg accttgtacc
      901 ccgcccccgc gatcggcgag atcaggtagc tcaggagggc gaccttgtcg cgcaggtacc
      961 cgacctcgcc gtagacgaag aagcggtcgg tcacggagta ctcgtcgcgg atcgtcgcga
     1021 cgagcttgtc ggcggtcgtt tcgccgttcg actcgctccg gaggaagagg gcgccgagct
     1081 tcacgacgtt cttcgtcttc gggtcgtact tcaggtcgcc cgagaggttg aagctcttcg
     1141 cgtcgctgtt gccggagctg atcgagaggc cggcgccgaa gctcccgtgc cagccgggcg
     1201 gcggctcgtc ggccgcggcg gagagggagg cgaggaggag gacggagagg acgacggcct
     1261 tcttcatgcc gctctccgtc acttcgactt cagcaggtcg cggatctcgc cgaggagctt
     1321 ctcctcggcg gagggctcgg gcggggcagc cggggccggc ggggcctcgc gcttcagtcg
     1381 gttgatctgc ttgacgacca tgaagatcgc gaaggcgatg atcaggaact cgaacgccgt
     1441 gttgatgaag acgccgtagt tcagcgtcgc gagccccttt tccttcgcct cggcgaggga
     1501 ggcggcggga gtgccggaaa ggttgatgaa gaggttcgtg aaatcgacct tcccgaggag
     1561 gagaccgatc ggcggcatga tgacgtccgc gacgaggctg ttcacgatct ttccgaacgc
     1621 gccgccgatg acgacgccga cggccatgtc gatgacgttc cccttgatcg cgaactcctt
     1681 gaattccttc agcatgtgtc actccctttc cgtgccggcg gccttcgtcc cgtgcccggc
     1741 cctcgtacct cgcaaacgag ggagcggcga tcggctgccg agacggaggt aatttagcat
     1801 ccgcggcggc ggcggacgcc cgggtgtccc cgccgggaac aggaggcggt gcatggcgga
     1861 cgacgtcggg cgggtcgacc gggagcggcg caggctcgtg gcgctcctcg ccggggcggg
     1921 ggcgggagcc gcggtcttcg gccgggcgct ctccgcgctc gcggacgggg cggcgaaggt
     1981 gaccgccggg atggtgaagc aggcggaatg gatcgccggc gtgacgttca ccgacgccga
     2041 gcgggagctg atgctctcgg acctgaacga gacggcggaa gggctcgccg cgctccgcac
     2101 cgtcgcgctc gacaacgcgg tgcctccggc gatcgccttc tcggccggga ccgccccgcc
     2161 ggccgacgcg ggacccgctc ccgcgccatc gcggcgggag cgccccgccg tgcgcccgcc
     2221 gtcggacgac gacctcgcct tcgccggggt gcgggagctg ggacggctcc tccgcgcgcg
     2281 gaaggtctcc tcgacggagc tgacgaagct cgccctcgca cgcctgcgga cgcacggccc
     2341 ggccctgtcg tgcaccgtga cggtgaccga ggagctggcc ctcgcgcagg cgaagcgcgc
     2401 cgacgaggag ctcgccgccg ggaaggaccg cggccctctc cacggcatcc cgtggggcgc
     2461 gaaggacctg atcgccgtcc ccggctaccc gacgacctgg gggtcccccc tgttccggga
     2521 gcaggtccgg ccggagacgg cgacggtcta ccggaggctc gaggaggccg gcgcggtcct
     2581 cgtcgcgaag acctcggtcg gagagctcgc ctggggcgac gtctggttcg gcgggacgac
     2641 gaagaacccg tggaagaccg accaggggtc gagcggctcc tccgccggct cggcctcggc
     2701 cacggcggcc ggcctcgtcg cgttcgcgat cgggacggag acgtggggga gcatcgtctc
     2761 gccctgcacg cgctgcggcg tcaccgggct ccgaccgacg ttcggccggg tcagccgcca
     2821 cggggtgatg gcgctctcgt ggacgatgga caaggtcggg cccatcgccc gctccgcgga
     2881 ggacggcgcg ctcgtcttcg cggcgatcgc cgggaaggac ccgctcgacc cggtctcgac
     2941 ggacggcccg gtcccctggc cggcgcgccg gagcgtgtcg gagctgaagg tcggcttcgt
     3001 cgaggagctc ttcgacgagg accggagcgc ggggacggag aacgaggagg cgaaggcccg
     3061 cgcggcggag tggcgcgact tcgaccggag gagccttggc gtcctccggg agctcggcgt
     3121 ccgcctcgtc ccggtgaagc tcccctcccg gacccccgtc gccccgctcg ggatgatcct
     3181 gacggccgag gcggccgccg cattcgacac cctcgtcctc gacgggcgcg tgaaggggat
     3241 ggtccggcag accgccgacg cctggccgaa cgtcttccgc cagggccggc tcctcccggc
     3301 ggccgactac ctccgcgcgc agcgcgtccg gacgctgctg atgcgcgaga tggaggagtg
     3361 cctccgcgac gtcgacctcc tcgtcgcgcc gacgttcggc ggcgacgcgc tcctcctcac
     3421 gaacctgacg gggcacccgt gcgtcgtcgt cccgaacggc ttccgcctct ccgacgggac
     3481 gccgacgagc ctgacgttca ccggaaagct cttcggcgag aacgacctcc tcgcggtggc
     3541 ggacctcttt cagcgggcga cggacttcca cacgcgccgg ccgaagctga cggaggcggc
     3601 cgcggcgaag gggtgaggcg aggcggtccg gccgcgctcc tcgcgtcgcg gccggctcgg
     3661 cctcagaatc gacgggatgt ccctttggtc cctcccggca cggggcctgt tcgtcgccgc
     3721 gctcctcgcc ggcgcgggcc gggcctccgc cctcgacccg gcgaccccgc tcgggcagta
     3781 cgggctcgac gcctggcagg aggggctgcc gcagaacagc gtccacgccc tcctgcagtc
     3841 gcgcgacggc tacctctggc tcgggacgta cgaggggctc gtccgattca acggggtctc
     3901 cttcgtcgtc tacgacacgc ggaacacgcc cgcgctcaag agcaacagcg tctgggcgct
     3961 ccacgaggac cgcgacggga cgctctgggc ggcgacgctc ggcgggggcg tcgcgcgcgt
     4021 gcggggaggg gtcttcgcgc ggtacggcgt ccgcgacggg ctgccgtcgg agtacgtctg
     4081 ggcgctcgcc gaggactccg agggcgccgt ctgggccggg accgacgccg ggatcgcccg
     4141 cttcgacggg acgtccttcc gggcggtgac gctcccgagc ccgttcgagc gggtgccggt
     4201 gcggagcctc ctcgggagcc ccgcgggcgg gctctggatc ggcacgacga ccgacggtct
     4261 cctgcgctgg gacgggacga cgctgcgccg gatccacccg cgtgagggac gggctcccgg
     4321 cgccgtctac gcgctcgcgc cggccccctc cggcgtggcg gccgcctcgt tcgggacggg
     4381 gctcgtggag gcgacggacg acgggcgcgt gaccgtccgc gggcggcgcg acggtctccc
     4441 gaacgacctc ccctgggcgc tcctgcgcga cgggaccggc gcgctctggg tcgggaccga
     4501 cggcggcggc gtcgcccggc tgtcgggagg gcgcgtggag acgctcaccg cggccgacgg
     4561 cctcgcccac tcgttcgtcc gctcgatcgc cgaggaccgc gaggggagcg tctggatcgg
     4621 cacgaacgga gggctcaacc ggctgcgcga ccggaaggtc gtcgtctacg gcgcgcgcgc
     4681 gggcctgccc gacgagaacg tccggtcgat ggcccggacg accgacggga cgctctgggt
     4741 cggcaccgat tccggcggga tctccgccct tcgcggcagt cgcttcgtcg tcccgcccga
     4801 ggccgcgccg ctccgcggcg tcccggtccg cgcccttctc gccggaacgg ggaacgacct
     4861 ctggatcggg acgaacggcg gcggacttct ccgcctctcg agggggcggc tgacccgctg
     4921 gacccggaaa gaggggatgc cctcggatat cgtctacgcc ctcgcgatcg accgggccgg
     4981 agacgtctgg gccgggacgt acggcggcgg cgtcgcccgt ctcttcggcg gacgcatcga
     5041 ggtgatcgac cgctcccgcg ggctgccgcg cgacgtgatc cgctcgatcc accacgcgcg
     5101 cgacggagtg acctggatcg ggaccgacgg cggagggctc gcgcgcctgc aggacggctc
     5161 cctctccgtc ctcgactcgc ggcacgggct cgcgaacgac accgtcttct cgttccacga
     5221 ggatgccgag gggctcctct ggatcggcac ggcgggcggc ctctcggtcc tccgccgtga
     5281 cggccgcctc cggtccgtcg gactcgccga cgggcttccc gaggagaggg tcttcgcgat
     5341 cctcgagggg gacggagacg ggctcttcat gagctcgaac aaggggatct tccgcgtctc
     5401 ccgcgcggac gtcctcgcgc ggatggaggg gcggcgcggt ccgttcccct ccctcaccct
     5461 cggtcggtcg gacgggatgc cgatcgcgca gtgcaacggc gcctcgtggc cggccgcgct
     5521 gcgcgacccc gacgggaagc tgtggttccc gacgctgcgc ggcctcgtcg gcgtcgaccc
     5581 cgcgcgcctc ccgtcgaacc cgctcccgcc gccggtcgtc gtcgaccggg tcctcgtcga
     5641 cgggcgcccc gtggcgggcg cgccgccggt cgtcctcccg ccgcggagcg ggaaggtcga
     5701 gatccactac gacgccctga gcctccaggc cccggagaag gtccgtttcc gctaccggct
     5761 cgagggcttc gagacctcct gggtcgacgc cgggccgcgg cgctccgcct tctacacgtc
     5821 gctcccgccg ggagacttcg tcttccgcgt cgttgcggcg aacaacgacg gcgtctggaa
     5881 cgagacgggc gcccgtttcg cgttccggct cacgccgggg ctccaccagc ggaccggctt
     5941 tcaggtgggc gtcgcggcgt tcctcctcgc cgtcgtcgga accggctacg cgctccgggt
     6001 ccgcggcctc cggagccggc aggtggagct ccagcggctc gtcgacgagc ggacgcggag
     6061 cctcgtggag gagatccaga gggccgaaga gctgcgccgc caggcggagg cggcgctccg
     6121 cgaggcggcg gaggcgaacc gcgccaagag ccgcttcctc gcgaacatga gccacgagct
     6181 ccggacgccg ctcaacgcga tcctgggcta cgccgagctc ctcgccgagg agccggagat
     6241 gggcgccagc ccgggcgctc gcgaggacgt ggagaaaatc cgcgtcgcgg gagggcacct
     6301 cctccagctc atcggagacc tcctcgacct ctcgaaggtg gaggcggggc ggatggaggt
     6361 cgtcgcggag ccgttcgcgg tgcgggaggt cgtcgaggag acggtccgga cggtgcgcca
     6421 cctcgtcgag gcgaaccgga acgccctcct cgtgacgatc gacgacggcg tgggcgagat
     6481 gacctcggac gcgttccgtc tgaagcaggt cctcttcaac ctcctctcga acgccgggaa
     6541 gttcaccgag gcgggaacgg tgacgctcga cgtcctgcgc gagcgtcgcg ccggcggcga
     6601 ggtcctcgtc ttccgcgtgg ccgacaccgg gatcgggatg agcccggagc agctcgagaa
     6661 ggtcttcacc ccgttcgtcc agggggacgc ctcgctcacg cggcggttcg gcggcaccgg
     6721 gctcggcctc gccctctccc ggcatctggc gcgcctgatg gggggcggca tctgggtgac
     6781 gagcgcggag gggaagggaa cggcgttcgt cctggagctg ccggccggga cgcccgaacc
     6841 gcacccggct tagcgaggcg ggcgacgccg cagcggcgac tctcgtcgcg ggccgcgtca
     6901 accggccacc gggcggccgc gtcgataggg cagcccaccg aaagagcggc gacggagccg
     6961 aacgggctgt cgcagcgaca tacggatcgg gagaaggagc ctcgtatgaa gcactcctcg
     7021 agatcgaacc tcgtccccgt ggcgacggcg ctcgcgctcg ccctcgtcgc gggccccctc
     7081 ctcgcgctcg acgcgccgcc cgacccgccc cccgggccgc aggggccgct cgcgggctac
     7141 ctccgctgcc tcggcatcgt cgggctcacg gacgcgcaga aggccgacgt caagtcgctg
     7201 ctcgaagcgg cgaagccgga gatggaggcg atccgcgagg cgctgaaggc cgaccgcgcg
     7261 acgctccgga cggcgctcgc ggcgacgccg cccgatccgt gcgccgtcgg gacggcactg
     7321 ctcgaggtcg aggccgacgt gaaggcgatc cgcgaggcga cgaaggagct ccggaccgcg
     7381 atcgaggcgc tgctgacgcc cgtgcagaag gcgaagctgg aggggtgcct gaaggcgccg
     7441 cggccgaacg ggccggcgga cgaggacggc gagggcgcgg agctctgacc cggaagcgtc
     7501 tcggacgtcg cgcccccggg gtccgcgccc cgggggcgcg cctacccggc cggcccgggc
     7561 ggcggcgcga ggacgacccg gtcccggccc aggcgcttgg cctcgtagag ggcggcgtcg
     7621 gccctttcga gaagcgcctc cgtcgtctcg ccccgagcgt gctcggcgac gccggcgctc
     7681 gacgtcacgc gctcggggag cccttcgacg acgagctcgg cgacccgcgc gcgaagcctc
     7741 tcggcgacct cggccgcgcg ctcgcggcgc gtcgccggga ggacgaggag gaactcctct
     7801 ccgcccatgc gtgcggcgac gtcgtacggc cgcacgccgc cgcgcagggc ttcgccgacc
     7861 gccgaaagga gacggtctcc gacggcgtgt ccccaggtgt cgttgacgcg cttgaagcgg
     7921 tcgaggtcga gcgcgacgac ggagagcggc tccccggtgc gctcggcccg gtccctctcg
     7981 tgggcgatcg cctcctccag cccgcggcgg ttcacgagga gcgtgagggg gtcggtccgc
     8041 gccagcttct cgatcacctc gttcgcggcg cgcagccgct cgagggtccg ctcgagctcg
     8101 cggccgcggc gcaccgcctc gcggttcgcg gtggcgagct cgttcgtcag ccggaggagt
     8161 tcctcgccga ggcgcgcctc ctcggcggcg agcctcgcgc ccgcgacgag gagaccgtcc
     8221 gggccggatt cgagaagcgc ccggaccgtg acgacggagc cgtcgaccgc gacgagattg
     8281 aggagccgcg gcccggaccg gcaggcctcg tccgagcgga acggagggag ggagtcccct
     8341 tgcccctcgg cgagcagctc cgcgagagga cggccgagga ccgcttcggg cgaaaggccg
     8401 agctcgcgga gggcggccgc gccgtgccac gcgacgcgcc catccgggtc ggtcgcgaag
     8461 aggacgacct ccggcgaggc gtccgcgagc gcgaggagaa gccgcgcgcc tttctccgcc
     8521 cggtcgccat ccggcgagga gggcgtcatc cggccgggcc ttccggctcg cgccgcgcgg
     8581 aggcggcgag ggcgtccctc acgaggacgt ccacggcctc gttccaggtc gcggggtggc
     8641 gcgggccgcg ataccactcc gtcacggcgc ggacggtccc ggcgaggccc gtcgcctcgt
     8701 cggccgcggc ggcgaggagg aggcccgcgg ccgcgcgcca ctccgccccg gagccgtgcc
     8761 cgcacgaggc gacgaaggtc tccgcgaggc gcgcggcgac ctcctcgggg agcgcctccc
     8821 tccggcggcc cgcgagctcg cgcgcggccg cttcgaggac gcctgcgtcg cgcgggacgc
     8881 cgcccgcccg gagctcctcc gcgaggagga cgagctggcg ctccaggagg agcgacggca
     8941 ttcccttccg cgaaaggacg ctcgcgagcc agccgacctg gccggagacg tcggcgggcg
     9001 ggagggcggt cagcgagacg agccaggccg cgtcgctccc ggtgaagcgc cggccccgct
     9061 ccccgtaccg cgagaggaag tacggatgct cctcgaggca tcgcgctccg gcgcgaaggg
     9121 cggcggccat ctcggaaggg tcggtgggga ccggatgggc cccggcctcg gggttcaggg
     9181 tcgtcgcgag gaagcgtcgg agaggtcgcg tcgcggggtc gagcaccgcg tgcaccctct
     9241 cgaggccctc ggcgcacgcg accgccgctt cgaccgcgga cgccgcttcg cccgacgcga
     9301 gggcgagctc gtcgagcacg gagcagaatc ggcggaacgc gggaccggtc ccggctccgt
     9361 gcccctcgta ccagccggtg ccgccgcccc cggcgcaggc gcgggcgtcc tcgaggtgga
     9421 cgaggttccc cagcgtcatc ccggtcgaga cgtagagcgg gccgaggagc gccgccggcc
     9481 gttctgccga gaggagcagg agcgcccggg agagctcgag cgcggtggcc gccggcgccg
     9541 ggtcgtcggg gagcgggccg cggcggtcga aggaggcgag gtccgagagg agggcctcga
     9601 ggcgggaggg gaccgcgtcc cgcgcggccg cgaccgcggg atggcgggac gagaggagca
     9661 ccctctcgag cgtcgcctcg acgaccgcca tcgcgcggag ctggtcgacg tacctctcga
     9721 gagggagggt ccgcgcggag agcgccgcga agaacgggag ccgttcgatc cgttcgtgga
     9781 tcgggccgac ggcctcgcga aggcggcccg agagcgggag agtgggcgtc tccatcggca
     9841 tcaccgtcct ccgtccgctt ccaggctccg ggccacgacg atcgcctcgc gcatgtcggg
     9901 cgcccaggcg tccgcgccga cgtcgagcga gcccccgccg gaggcgcgga acgcgccgcc
     9961 gccgacgagg atgcgggggc gggactcccc gaacctcgac cgtaccgcgt cgacgagcgc
    10021 ggagaccttc gggacgttct gccggagcgt gcaggagatg ccgaacaggt cggcgcgatg
    10081 cgcggcgatc gcgtcgagga cgtccgcccc cgggacgtcc gtgccgagga agcggacgtc
    10141 ccagccgtcc gcctcgagcg cgtcggcgat catgttcccg cccacctggt ggagctcccc
    10201 ctcgacgccg gtcaggacga cccgcccgcg catcgtctcg gagcgcggga gcttctcgta
    10261 gaggcgcgcc acgacgagct gcgtgatcgc ggtcgcgagg tgctccgtgg cgacggagat
    10321 gcggccggcc tgccagagcc ggccgacctc gtagagcgcc tcctgcagga cgtcgacgta
    10381 gacgtcgcgg acgtccatgc cccgctcgag agcgccgagc gccgcctcga ccgcgtcctg
    10441 gcggcgcccc gcgaggatcg cgtcgagata ccgctccctc tcggaacgca tgccgctcct
    10501 ccctttcggc gcgccggtcg acacggcccg cacgtccctc cggtccgagg ctccgtaccg
    10561 gggagtctga tacggatcga ccggccgcgt cgagcggagt cccgccgtcg gagacccgga
    10621 ggccggaagg ccgggtcccg cacgccggcg gccgggatcg cggaggaggc gctctcccgg
    10681 tacgctccgc ccgatggaac gggagagcga gggcgtcttt cggggctggc ggcggcccgg
    10741 gccgcggccg ccgggcggac tcgagccggg cgaaggcgag acgctcgact acctctgcgg
    10801 gagcttccgg atcttccagg cggcgcgcgg ccaccgctac tccacggacg acctcctcgt
    10861 cgcctggtgg ggcgtcggga acgccccgcg cgtcgacagg ctcctcgacc tcggctccgg
    10921 catcggctcg gtcgcgatgg cggcggcgtg gaagctgccg ggggcccgtg tcgtgacggt
    10981 ggaggcgcag gcgatctcga tcgccctcgc gaggaagagc gtccgctaca acggcctcga
    11041 ggagcggctc cgcgtcgtcg agggggactt ccgcgatccg gccctgctcg agggcgagcc
    11101 gccgttcgac ctcgtcctcg gggcgccccc ctacttcgag aggggaacgg cgaccgaggc
    11161 gcgccatccg caggcggtgc cggcgcggat caccctgcgc ggcgggatcg gcgcctacgc
    11221 ggaggcggcg cggcggacgc tggccccagg ggggcggttc gcgttcgtct ggcccgtcgc
    11281 gcgcgacggg gaggcccgcg acgcgctgag gcgcgagagc cttctcctcc tcgcccgccg
    11341 ggacgtcgtc ttccgggagg gggagccgcc gctcgtcgcg ctctacgccg ccgcccgcgc
    11401 cggggacctg ccggcctcac tcgcgaccgc ggccggcttc ccgtgcgtcg agccgccgct
    11461 cgtcgttcgg cgtgcgaacg ggcgtcacac gcctgaatac gcctccgtgc ggctctcgat
    11521 gggattcccg cccggggacc tcgcccctcg gcccgccgcc tgactcgggc gcctttcgcc
    11581 ggccccgaag gggtggatgc cgtatcgtcc tcccacacgt ggtcccagat cctccgcagg
    11641 cgctgctgcc gccgctgacg acgatcccgt tcgggctcgg cctcgacgga aggccgatcc
    11701 ggcacgtcaa cgggaagctc gtcgtcggga cgatccggca cgcgatcgac ctgacgctgg
    11761 agcgggagct cgacgcgctc cccgcaatgc cgtcgccgac cgagcggaac gcccgggagg
    11821 ccgtcgtgcg ggcgcgcctc ctcgtccggc tcgtcgagag gctgaacggc ctgatccccg
    11881 acccgaagta ccacgtcacc tcggagacgc tcctcgtgga cgggaacaac tactctcgcg
    11941 agttcgagct ctacgtgaac gagctctgcc gcgagttcgc cggcgtcgag tacttctact
    12001 tcacgcgggg ggggaaggtc gtccccccga cgatcgccgc gatcttccgc cccttcggcg
    12061 tccggcaggc atacgcgctc ctggcgcgcg ctgcggagaa gttcgtcgag acggacctcc
    12121 gggtcgtcga gacgacgacg cgttcggccg tcgtgaggtg gtacgcggcg acgcagctcg
    12181 aggacgtccg cgacgagctt caggaacggt ggctcctcgc cagctgcgcg gcctaccagg
    12241 ggacctgcgc cgcgattccc gcgctgccct acccggggca gccgtgggcg gaggtcgacg
    12301 agctgaagtg ccaggcgcgc ggggacgagt gctgcgaatg gcgcttcacc tgggaggacc
    12361 cccacccgca cgcggctcac ctcctgctcg ccggcgccgc cgcgtcgacc ggacttctcg
    12421 gctgggcgct ctccggctcg cccggctcgg cgctcgcggc gcccgcgatc ctccttcccc
    12481 tcctcggcgc ctggcacgtg gcgcgcatcc gcaggctcca gcacgaccgc caggagcagg
    12541 accgcctcat cgccgcgcag cgggccaccg ccgagagcca ggtcgaccgc gtccaggccg
    12601 cgtacgccga ggtccagcag tcgacgctcg cgctgcgcgg gaaggtcgcg gagctctcgg
    12661 cgctgcgcga ggtggcgctc gcggtctcgg cctccctcga cccgttcgag gtcgtcgacc
    12721 ggagcctcga ggcggtgcgc aggcgcctcg actacgaccg ggcggtcgtc ctcctcgtcg
    12781 acgagacccg caacgtcctc tcgcgggcgc gcagccgcgg cctttcgccc gaccaggctt
    12841 ccctcctcgc ccgggtctcg atccgcctcg acgacccgct ctccgcgatc gcgcccgccg
    12901 tccgcgacgg cgtcgccttc cggctccgcg acctgccggg gccgcccggc gccgcgacgc
    12961 ggcagctcgt cccgatcctc ggctcggagg aggtcttcgt ggcgccgctc ctcgcgcgcg
    13021 ggcggagcgt cggcgtcctc atcgtcgaca acgggacgac gcgccggccg ctccccggcg
    13081 aggggctcga gctcctgacc gcggtcgcgt cgacgatcgg cgtcgcggtc gagaacgcgc
    13141 acctctactc ccagatcgag cagcagcggc ggacgctcga gacccgcgtg cgcgaacgga
    13201 cggccgacct cgagcgtgcg acggaggagg cggagtcggc gaaccgggcc aagagcgcgt
    13261 tcctcgcgaa catgagccac gagctgcgga ctccgctgaa cgcgatcatc ggctacagcg
    13321 agatgctcct cgacgagagg gcgggcgccg acgcggacgg tgccgcctcc gacctcggga
    13381 agatccgtgc cgccggcctc tacctcctcg ggctcatcaa cgacgtcctc gacctgtcga
    13441 aggtggaggc ggggaagacg gagctgaacc tcgagccgtt ctccctctcg gcgctcgtcc
    13501 gcgacgtcgc gagcgacgtg cggccgctcg tcgagagagg cgggaaccgc ttcgtcgtcg
    13561 acgcgccctc cgacgagacg ctccacggcg acccccggaa ggtgcgccag gtgctcctga
    13621 acctcctcgg gaatgccgcg aagttcaccg agggggggac ggtcgagctg cgggcgcgcc
    13681 gcgacgggga ggaggtcctc ctctcggtcg cggacaccgg gatcgggatg acgccggacc
    13741 aggtcggccg cctcttcacg gcgttctggc agggggaccc cgcgtcggcg cgcaactacg
    13801 gggggaccgg cctcggcctc gccatcagcc gccactacgc gcgcctgatg ggaggggacg
    13861 tctccgtcgt cagcgaggcg gggcgcggct ccacgttcac gctccgcctg ccggtccggg
    13921 tcgcgggcgt ctcgtcttcc cgttcggcgt aagctcggcc gaaaagcgcc ccggacggac
    13981 ggccacccga cgcgaaagga cccgcagcgg atgtcggact acacccagtc gaacaacacc
    14041 ttccgcatca ccacgcccct cgggccggac gccctcctgc tccggaggat ccgcggctcc
    14101 gaggggatct ccgagctctt cgacctcgac ctcgaggtcc tcgccccgaa cgagaccgag
    14161 gtcccgttcg acgcgctcct cgggcagtcg gtgaccgtgg cggcctacgg cgtcgacggg
    14221 agcccggtcc ggcacttcaa cgggatctgc gcggagctcg aggcggggcc gcgggagacg
    14281 atggaggccg acaagaagac gttcacggcc taccggctcc gcgtcgtccc gaaagtctgg
    14341 ctcctcggcc tcgtccaccg gagccgcgtc ttccaggaga tggcggtccc ggcgatcctg
    14401 aagatcgtcc tcgacgggat cgacgcctcg taccggctcg agaggacgta cgagccgcgg
    14461 gagatgtgcc tgcagtaccg cgagagcgac ctcgccttcg cctgccgcct gatggaggag
    14521 gaggggatcc gcttctactt cgagcactcc gacgggagcc acctcctcgt cgtgacggac
    14581 cgcggagggc acgaagacct cccgggcccg agcccgctcg gctacgaccc tctggaaggg
    14641 ggcggccgcg acgaggaccg gatcacgtcc tggcacaagc ggcagtcgct gacgcgcggg
    14701 aaggtcacgc tccgggaccg ctccttcgag ctgccgacga acgacctcga gaaggtgatc
    14761 ctcctcaagg actcgctgaa ggcggggacg gtgacgcaca agctccgcct cgccgaggcg
    14821 gacaagcgcg agctctacgc gttcccggga ggtcacgcga agccgttcga cggcgtcgcg
    14881 cccggcggag gggagcgcgc cggcgagctg aacaagacct tcccctacgg cgagacgcgg
    14941 gcccgagtcg tcatggagcg ggaggagacg cgggcgatcg agatcgaggg ggcgagcaac
    15001 gtccgccacc tcgtccccgg gttccagatc aagctcgaca ggcaccacga cggcaacggc
    15061 tcctacctcg tcacgcgcgt ccgccacgag gggcagaacg gctcgttccg ctcggacgag
    15121 gtggagacgc ggtacgagaa cacgttcacc tgcctgcccg cggacgccgt ctaccggccg
    15181 ccccgcctga ctcggcgccc cgtcgcgcag ggcccggaga cggcgatcgt cgtcggcccg
    15241 tcgggcgagg agatctacgt cgacaagtac gggcggatca aggtgcagtt ctactggcac
    15301 cgggagggga agcgggacac gacgagctgc gcctggctcc gcgtcgtgac gcccctcgcc
    15361 ggcgcccagt gggggagcta cgccttcccc cggatcgggc aggaggtcct cgtcgcgttc
    15421 cagcacggcg accccgacga gccggtcgtc ctcggcgcgc tctacaacgc ggagaacatg
    15481 cccccctaca cgctcccggc gaaccgggtc cggacggggc tgaagacccg gtccacgctc
    15541 aagggcgcgg ccgacgagtt ccacgaggtc cgcctcgacg acacgaagga cagggaggag
    15601 atcttcgtcc agtcccagaa ggactttcac cgcctcgtca agaacaacga cctcctccag
    15661 gtcggcgtga agaagggcga caaggagccg ccggacggca gccagaagca ggagatctgg
    15721 aagaaccgga cggtccacgt gagcaagggg gacgacttcc tgaacgtcgg gcggcttccc
    15781 ggcgagacgc agccggccgt cgattcgcgg tcggggtccg atcccggcca gtccccgtcg
    15841 tgcggcggga acctgacgac gtgggtcgcg aacgaccgga cgacgactct gaacacgggc
    15901 aacgacactc tcgtcgtcgg cctccaggcg atgggcaagt cgacgccctc gctgtccgtg
    15961 catcagggca accaggtcct cgagatccag aacgaccgga ccgcgacggt caagtacggg
    16021 aacgacagtc tgacgatccg gaaggggaac ctcgacatca agctcgaggg ctcgatgccc
    16081 gacgggaagg tctcgatcga ggcgccgaac gccatcgagc tgaaggtggg gatgaaccac
    16141 gtgaagatcg acacgatggg gatcgagctg aagttcggga cgacgcagct gaagctcgac
    16201 gccatggccg cggagctgaa gacgacgacc ggctcgctga agctcgacgc cgcgagcgcg
    16261 acgctcaagg ggatcaacgc gacccttcag gggaacgcca tcgccgacct caaggcgccg
    16321 atcgtcaacg tccgggccga cggcgtcgcg agcgtcggcg gcgcgatgac gaagatcggc
    16381 tgaggaggga cggatgagca gcaccggcgc gagcccgcga accgccgccg ccacgatcct
    16441 cccgaggctc gacgcctccg cggaggcgaa gggcctcctc gcccccgacg cggaggcctc
    16501 cgagtacgtc gccctgctgg tggagaagga gctcttcagc gacgccacgg gcttcgtcgc
    16561 gcagtggctc ccggcgcacg acgcggtcgc ctgggcctgc gaggcggcgc ggaccgcgct
    16621 cggcgaagcg ccgcccgccg agcgcgcggc gcttgaggcg gccgagcgct ggctcgccac
    16681 gcgcgacgag aaggagcgcc gcgccgccgc ggaggcggcc gagcggctcg gccaccgttc
    16741 ggcgcccgcg atggcggccg ccgccgcggg ctggacgggg gggagcctcg cgcccgccga
    16801 cctgccggcc gtggcgccgc cgccgggcct cgccgggaag ggggcttccg cggcggtcct
    16861 cctcgcggcg gcggccgccg cgcccgaggc gcccgagcgg ctccgcgccc tgatctggag
    16921 cggcgtcgac ctcgcggagg gacgccggaa gccgggcctc gccggcgcgg cgcgcgggcg
    16981 ggccggacgg gagggctgaa cgatgccgca gcccgccgcc cgcgtcggcg acatgcacac
    17041 ctgcccgatg cagacaccgg ccgtcccgcc ggtcccgcac gtcggaggtc cgatcctgcc
    17101 tcccggatgc ccgacggtcc tgatcggggg ccttcccgcc gcctgcgtcg gagacatggc
    17161 ggtctgcgcc gggccgcccg acgtggtcgt gtccggcgcc ttcaacgtcc tcgtcggcgg
    17221 acgtcccgcg gcgcggatgg gcgacccgac cgcccacggg ggcgtgatcg tcgtcgggta
    17281 tccgcccgtt ctcgtcggct gaaggaagga gtccgccgag ccgccgtccg cgtagactgg
    17341 acggaccggg aggaggggag ccatgccgga cgggacgtcg cctccgggca cgtgggactt
    17401 cgtgatcgtc ggctccgggt tcggcgggag cgtcagcgcg ctccgcctct cggagaaggg
    17461 ctaccgggtc ctcgtcctcg agaaagggcc gcgccgcagc gcgaacgact tcgcgaagac
    17521 gacgtgggac ctccgccgct ggctctggat gccgggactc ggctgccgcg gcatcttccg
    17581 gatgacgttc cttccgcacg tcaccgctct ctccggcgtc ggcgtcggcg gcggctcgct
    17641 cgtctacgcg aacacgcttc cggttccgaa gcgccccttc ttcgaggcgc cgtcgtgggc
    17701 acggctcgcc cgctgggagg agg
//
LOCUS       DAOTEZ010000027        17517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2189_length_17517_cov_6.646432, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000027 DAOTEZ010000000
VERSION     DAOTEZ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17517
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2189_length_17517_cov_6.646432"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="P5164_02440"
     CDS             complement(<1..454)
                     /locus_tag="P5164_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42781.1"
                     /translation="MRARARLASKCIVLALLATASPAATAQESGGLATGDGRFRLKGE
                     VKVHFRHSKDYYVTVGRIGNVDIDQRTTDPGGSFEVSTVNLIGEGDLTDGLKAKVEIH
                     FIDLYNRNPTSTGDEVRVREAWLRFGKRVDGLKAFEGTSLYVQAGKAPR"
     gene            597..1151
                     /locus_tag="P5164_02445"
     CDS             597..1151
                     /locus_tag="P5164_02445"
                     /inference="COORDINATES: protein motif:HMM:NF019494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HRY42782.1"
                     /translation="MTQPVVVRKYENRRLYDTSSSRYVNLPEIAQLIREGTEVQVVDA
                     KSGDDITAVILTQIIHEDARAKRGDLPIPFLRELVVASDRAFRDFVVWYADAALSAQR
                     GATEAFPPLAHVLPFLERTLEGLKVKAAPAAKPEPADDVAALKERIAELEERLGAARP
                     PRRKVPAAASAARRGGRRATPRRG"
     gene            complement(1132..3306)
                     /locus_tag="P5164_02450"
     CDS             complement(1132..3306)
                     /locus_tag="P5164_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRY42783.1"
                     /translation="MRTATDAPSPDDVPTMVAPREGSSGASGPPLRGGSVPRPGGLRL
                     RTQFFLAAALLVVVTLGAAVALSTWRANRIAEESIREALRTIPGDVAVYRAGLESQLK
                     ATLRSVAEEPGIKAIFDSPPATVWDTAKDKAAILNARTFFFFDRAGVLIARSDRPPSE
                     EERRSFREVAWVSSPLEEWTESSATIREGAALAVVAAAPVVAGDPEKGEARLVGVVAA
                     SVPLDATRARELRDLTGGQVAFVAETGKKGEPTKLEVAAATEGFGAASLLPALDRDPS
                     VLARLLEGDADSAPLDLLVGGDRRVVKAVPLRAAGGETLGAVVVSRSREEETAAFRKI
                     RETLLLVGLGVLLVSVPVSFLLGGRIARPLQQLAAGAIAVREGELEVKLPSGGSGEVA
                     LLARAFEALVGELREKRQLEELLAEMRRRPADATRNLAPTSATAAGALPQDAALAAAR
                     GPRVGALFAGRYDVVSVLGRGGMGSVYQVLDRELDEEVALKVLTPEAFSEGTHATQTL
                     KQEIRLARKITHPNVVRTHDLGEADGVRFLTMEYVPGTTLREVLDRRGAVALAPGLQI
                     AKQLCRGLSAVHEAGIIHRDVKPQNIMVLPNGVVKLMDFGIARTDEGGDGALDGHTVG
                     TPYYMSPEQALGQRLDARSDLYTVGVVLFELFTGERPFPGTDAATVMQRHVAAQPPRP
                     SSLRPDLPEYLDRIVLACLAKRPERRPASAADLYAALTRVAA"
     gene            complement(3290..4243)
                     /locus_tag="P5164_02455"
     CDS             complement(3290..4243)
                     /locus_tag="P5164_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42784.1"
                     /translation="MSRLLVAALAALLLLQAPATGSWWKRRRPTPTPTATPTPAPAEQ
                     ADGAAPVDPGAVGTPGPVVPGTPDPGAGRDGEPVESARPRPEEPPAPVPSPAPSPTPR
                     PTATPRPTPTPIPTGTLRVRVLVAGADGRALPAPEAVVWLPGSRGRRAAPPPKMASRD
                     KRFEPRVLAVPKGAAVSFPNFDKIFHNVFSLSEPGRFDLGLYRNGAARTARFEEPGVV
                     RVYCNIHPQMAAYVVVLDGELYAQTGADGVAAIGAVPAGRHVVKAWDERGGEWSGSAS
                     VPAGGTTDVSVTLDASKWKATPHKNKHGKDYPPPDDDENRY"
     gene            complement(4261..5436)
                     /locus_tag="P5164_02460"
     CDS             complement(4261..5436)
                     /locus_tag="P5164_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017322278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HRY42785.1"
                     /translation="MTRRRPVYLDHQATTPLDPRVLQAMLPWLTEEFGNAASLQHAFG
                     WTAESAVNAAREEIADALGAQPREIVFTSGATEANNLAILGVARASRGRGGHIVTSAV
                     EHHAVLDPCRALEREGFEVTVLPPDREGRVSPESVEAALTDRTALVSVMAANNEVGTL
                     NPVDRIAALCAERGVAFHTDAAQAFGKVPLEAGGGVALASLSAHKLYGPKGIGALYVR
                     SRPRVPLAPILHGGGHERGLRSGTLNVPGIVGFAAAVRIARRERVAEAERLGRLRDRL
                     EASIRSSLDGVTANGPPSGPDRLPGNASLSFAGVDGTRLVAALRGVAVSSGSACTTAS
                     GEPSHVLRALGVPEPLGKATLRFGVGRFTTEGEIDEAAAEVVAAVRRLRSENESLRD"
     gene            complement(5433..5876)
                     /locus_tag="P5164_02465"
     CDS             complement(5433..5876)
                     /locus_tag="P5164_02465"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRY42786.1"
                     /translation="MLKLNRIVDYGILVMTEIARRNACVSTTELAAAFGVSATIVAKV
                     LKKLARAGLVSSERGSRGGYRLARPAAEVTLRAIVNALEGPISLTYCAAGMSNGRCRT
                     RPFCVATDAMRRLNHLVNGAFDAISLEDLLHVPVPDASGTTGSAP"
     gene            5992..7590
                     /locus_tag="P5164_02470"
     CDS             5992..7590
                     /locus_tag="P5164_02470"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HRY42787.1"
                     /translation="MPPFLSPLPRVRLVDAFARPFDGVVAAARTCYSPKGIVTVDDVA
                     GDPLADPAERARRRERRDALARDVYAAGHHTTFQHAHLQFTLENVSRQLVWSFLHAHP
                     HYNSEQTSQRYVEVKPGRYAVPALAGEALEVYRACCEMQTEAYRRLGDALAPRAARAF
                     FATFRGRASQPERWERAIRKRALEVARYALPVATFTTLYHTVPAVTLLRYRRLAETFD
                     APTEQRLVVEAMTDELLRHDPAWAAVLQEPIPLEETPEHALAERLFSAPRCDRPGDGS
                     FRSRFDASLDGRVSKLVDRKARNEELLAEAVREVVGVTPETLSDDDAIALAVDPAANR
                     LLGESLNLTEHGKLSRALHHPAYTFRKKLSHTADSQDQRHRTTPGSRPTLHAYLSDEP
                     DYVTPMLLEEPGEARTLYDETMARTWEAIARLGALGVPDEFRAYLLPNAVALRFTESA
                     DLAGLKHKLAMRLCWNAQEEIWRASVDEAEQVRDAEPRIGRWLLPPCGLRARAGAKPV
                     CPEGERFCGVLVWKQDLTAWRRLL"
     gene            complement(7565..8845)
                     /locus_tag="P5164_02475"
     CDS             complement(7565..8845)
                     /locus_tag="P5164_02475"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRY42788.1"
                     /translation="MRRGPDASRPPARRRSPARAAAALLLALAAAAPAASSAVAMGSG
                     GGSLPAAQPATWTGTASGEMKAQGAKTPLRHAYAVVVRPRNAAADETRLVLSEEPVPL
                     EVLEELLRRLPSFGFRGVEVVLGAGGGAPAVFFHHDELPAGLEVREVARLVPAPAAAG
                     RLAGRIVFDDPGFSFAFDATFDAPLFRPAAKPTRTTDPGLPSEEQAKATLEERGLELT
                     PSALDRVVRDGDIEAVRLFLAAGMPATTGEPGGSVLESAVERGHAEVVRVLLAAGADP
                     NGVGYAGRPLLIAAAEGQGADVVRALLAGGADVTRKGAGNATALHAAAEAGKLDVVEV
                     LLSAGAKVTARTTHGWTALHSAVRRGDLPMVRSLIAAGADVARDRKELLDLARSAKGP
                     DVEKAIRDAPVPRQRKTDLTPSGEGAVRGGGARR"
     gene            complement(8842..9549)
                     /locus_tag="P5164_02480"
     CDS             complement(8842..9549)
                     /locus_tag="P5164_02480"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRY42789.1"
                     /translation="MAIDRQDVDAVKELLAAGHSANTPIVHDTVASNPLVKACWRKNM
                     EIVRLLVEAGADVNGLEKDRRVNDRGEVHWFVKSPLEEVVGQDLEIVRYLISKGAKVN
                     EKLTDGRTVLFLPAYEGNLETLEVLVAAGGDVNVVDDIGYTPLKHAVMGRKPEAIRLL
                     VAKGAKVNQAPTKVNVGETALHVAIASGFPDIVQLLLELKANPNAKAEYSGTPLQQAR
                     KGDQDDVVAMLKAAGAK"
     gene            complement(9752..10033)
                     /locus_tag="P5164_02485"
     CDS             complement(9752..10033)
                     /locus_tag="P5164_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42790.1"
                     /translation="MRHLLVSSRRDRLADDLRALLPPSSVLLTARGVDATLERLARSA
                     RVDAVVTDDAAVRTAIREEVPGCLPVVLVPEAASASEAIRRIEEALEAP"
     gene            complement(10041..11030)
                     /locus_tag="P5164_02490"
     CDS             complement(10041..11030)
                     /locus_tag="P5164_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HRY42791.1"
                     /translation="MKEVRPESKRRAGAAGPVGGVALGAALLAVAALPLDGARNAPPL
                     RVVLPGLAPAYVERVPAAKAELLRVINRERAEHGAPPLAYDLLGAKVGDEFCEQAARD
                     RTTGHWDLAGRAPYLRWALAGGIDHHAQNFAAESRTGFDFTESPAELLLRMHAAFMAE
                     EPPNDGHRRTILDPIWTHVGIGFALVGGEMRMTEEYSRRVLEWAELPAGPVRAGGGAP
                     LALKLPKGWSVGAIEVAFAPVPNPMSRAEIAARASYGLPPPWRTLRPYPPAGTRWQSG
                     ESGDFRANAAGRIELTIPLDRGPGHYYIVVYAGQGIRSGKPLFPVAAPLLVAE"
     gene            11189..>11787
                     /gene="eno"
                     /locus_tag="P5164_02495"
     CDS             11189..>11787
                     /gene="eno"
                     /locus_tag="P5164_02495"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965800.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRY42792.1"
                     /translation="MYAIEEIHAREILDSRGNPTIEVECLLEGGASGRAGVPSGASTG
                     TREALELRDGDPKRYGGKGTLKAVDNVNKTIAPELLGFDARDQAGLDRLMCDLDGTEF
                     KSKLGANATLGVSMAVCRAAAEGCDLPLYRYLGGTGAVSLPVPMMNVVNGGAHADNPL
                     DHQEFMLVPAGFPSFREALRAGAETFHALKSILKKKGLAT"
     assembly_gap    11788..11887
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11888..12321
                     /gene="eno"
                     /locus_tag="P5164_02500"
     CDS             <11888..12321
                     /gene="eno"
                     /locus_tag="P5164_02500"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696954.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRY42793.1"
                     /translation="VSIEDGCAEGDKAGWKLMTEKLGAKIHLIGDDLFVTNPKILAEG
                     IRDGIANGLLVKVNQIGTITETLEAVRMAQVARYVTAVSHRSGETEDTTIADLAVATS
                     SMFIKTGSASRSERLAKYNRLLRIEEELADAGRFHGLAVKK"
     gene            12357..12827
                     /locus_tag="P5164_02505"
     CDS             12357..12827
                     /locus_tag="P5164_02505"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRY42794.1"
                     /translation="MRFERITGPKKGLVSRTGGRPVLPSRPARRLDEPSASGPAARPD
                     RPHAHPLSYVGFSLLLSFLLFVFFLAGDRGFLQVRKQKAQVAALQAEVSAIAGRNEAL
                     EKEIERLAKDPAAVERIAREDLQLVAPGDVVLVLPPGFEEKVTPTRPPAGVAAR"
     gene            12977..13438
                     /locus_tag="P5164_02510"
     CDS             12977..13438
                     /locus_tag="P5164_02510"
                     /inference="COORDINATES: protein motif:HMM:NF017262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HRY42795.1"
                     /translation="MSWFLQFAHLLATAAWIGGMIFMKAILVPSLAVLEPPQRGRLMG
                     SVAKRFTALAWTSTLLLAVTGLLKSPEGLLGDTATDVGLLLTVKVALFGVMVVVGTVI
                     TFAVAPKLVALAPAPGSTPSPAFVTAQKALDALSGANALLGLVIVGIVSAF"
     gene            13517..14749
                     /gene="kynU"
                     /locus_tag="P5164_02515"
     CDS             13517..14749
                     /gene="kynU"
                     /locus_tag="P5164_02515"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HRY42796.1"
                     /translation="MISRADAAALDAADPLASFRDRFVLPEGLVYLDGNSLGALPKGA
                     AERMVEVVSREWGAGLIRSWNDAGWIDLPRRVGDKIARLVGGEPGEVVCTDSTSVNLW
                     KVLAAALSLRPERRTVLSEEGNFPTDLYVAEGLARLLGQGHRLRLVPKGELLTSLDAD
                     VAALLLTHVDYRTGVRHDMERLTRAAHEAGAIAIWDLAHSAGAMPVDLRAAGADLAVG
                     CGYKYLCGGPGAPAFLWVPKRLQDSFVQPLSGWLGHAEPFAFERVYRPAEGVGRFLCG
                     TPPILSMSSLDAALDVILEADLAIVRAKSEALTSFFIEAVEQECGDAVRLVSPRVPSL
                     RGSQVSFTHPDAYAVMRALIERSVVGDFRAPDVLRFGFAPLYVGFVDAWDAAKALADV
                     LSSHAHERPELRRRAAVT"
     gene            complement(14751..15167)
                     /locus_tag="P5164_02520"
     CDS             complement(14751..15167)
                     /locus_tag="P5164_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42797.1"
                     /translation="MKNADRNILLAATAALALAVAPSALADLRIHVNLPLPPPPHEVL
                     RHAPLPPPPHVDVRVDRRDHRDRRDRWRDEDRRHRDRYRDDGWRNDHRYRDRDRWAFV
                     EGRWVRKPFLDAVWVNGYYDRWGRWVPGYWTRARYR"
     gene            complement(15362..16927)
                     /gene="ahpF"
                     /locus_tag="P5164_02525"
     CDS             complement(15362..16927)
                     /gene="ahpF"
                     /locus_tag="P5164_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HRY42798.1"
                     /translation="MLDASLSAQLQQIYSDLPVTVVLRQRPSEHPKNGELTTMLADVA
                     ATAPGKIVHRVEGEASAIPAFEVAAEGRAATILFRGIPGGHEFSSLVLAVKYAAGQGK
                     RPDEGIQSRIRHLKGPVRLRTYVSLSCHNCPEVVQALNLMAVIHPDFVHEMVDGGLAE
                     GEVEKLGIQGVPTVISLGHENAQVSVGRAGLAELLDALEERFGSAPSDAAQDLPVRDP
                     YDVLVVGGGPAGASAAIYSVRKGLRTALVAKNVGGQVLETLGIENLIGTTRTEGKRLA
                     ADLLAHLRSYPVEVLDNRRVEAIEDGELKTVRLAGGEVLRTKALVMATGAQWRELNVP
                     GEKEYIGRGVAFCPHCDGPFYKDREVVVIGGGNSGIEAAIDLAGICSRVTVVEFMNEL
                     KADKVLVDNLRKLPNTEVIVNHRTAEILGDGSKVTAIKVVDRATDAERTFPIDGIFIQ
                     IGLAPNSGLVRGLVDVNRFGEIVVDEKCRTSTKGIYAAGDVTTVPWKQIVIAMGEGAK
                     AALAAFEDRIHSA"
     gene            complement(17058..>17517)
                     /gene="ahpC"
                     /locus_tag="P5164_02530"
     CDS             complement(17058..>17517)
                     /gene="ahpC"
                     /locus_tag="P5164_02530"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HRY42799.1"
                     /translation="FFFYPADFTFVCPTELGDLADNYAEFKKMGVEIYSVSTDTHFTH
                     KAWHDASDTIKKIQYVMLADPTHILSKGFGVHIDEAGLAYRGTFVVNPEGQVKIAEIH
                     DNGIGRDAKELLRKVQAAQYVASHPNEVCPAKWTPGQETLKPSLDLVGKI"
BASE COUNT         2297 a         6515 c         6160 g         2445 t
ORIGIN      
        1 atcggggagc cttccctgcc tggacgtaga gcgacgtccc ctcgaacgcc ttcagcccgt
       61 cgacgcgctt tccgaaccgc agccacgcct cgcgcacgcg cacctcgtcg cccgtcgagg
      121 tggggttccg gttgtagagg tcgatgaagt ggatctcgac cttcgccttc aggccgtcgg
      181 tgagatcccc ctcgccgatc aggttgaccg tcgagacctc gaacgagccc cccgggtcgg
      241 tcgtcctctg gtcgatgtcg acgttcccga tccggccgac cgtcacgtaa tagtccttcg
      301 agtggcggaa gtggaccttg acctcgccct tcaggcggaa gcggccgtcg ccggtcgcga
      361 gcccgcccga ttcctgggcg gtcgccgcag gcgacgcggt cgcaagaaga gcgaggacga
      421 tgcacttcga cgcgaggcgt gctcgagcgc gcatgggagg cctccttccg acggtggccg
      481 gatcctacca aggctcgcgc ccgcggcgcg aggcgaccgc ggagcgcggc ccccgcccga
      541 cggcgacccc gcacctctcc tcccctacaa tccccgcaca acccggagga agcgtcatga
      601 cccagcccgt cgtggtccgg aagtacgaga accgccgtct ctacgacacc tcctcgagcc
      661 gctacgtcaa cctgccggag atcgcgcagc tgatccggga ggggaccgag gtccaggtcg
      721 tcgacgcgaa gagcggcgac gacatcacgg cggtgatcct gacgcagatc atccacgagg
      781 acgcgcgggc caagaggggc gacctcccga tcccgttcct gcgcgagctc gtcgtcgcgt
      841 ccgaccgcgc cttccgcgac ttcgtcgtct ggtacgccga cgccgcgctc tcggcccagc
      901 gcggcgcgac ggaggcgttt cccccgctcg ctcacgtcct gccgttcctg gagaggacgc
      961 tcgaggggct caaggtgaag gcggctcccg cggccaagcc ggagcccgcc gacgacgtcg
     1021 cggccctgaa ggagaggatc gccgagctcg aggagcggct cggggcggcg cgtcccccgc
     1081 gccgcaaggt gcccgcggcg gcgagcgcgg cccgccgggg cggaaggcgc gctacgccgc
     1141 gacgcgggtg agggccgcgt agaggtcggc ggcgctcgcc gggcgccgct ccggccgctt
     1201 ggcgaggcac gcgaggacga tccggtcgag gtactccggc aggtcgggcc ggagcgagga
     1261 ggggcgcggc ggctgcgccg ccacgtggcg ctgcatgacg gtcgccgcgt cggtccccgg
     1321 gaaggggcgc tccccggtga agagctcgaa caggacgacc ccgacggtgt agaggtcgct
     1381 gcgcgcgtcg agccgctgtc ccagagcctg ctccgggctc atgtagtacg gcgtgccgac
     1441 cgtgtggccg tcgagggcgc cgtccccccc ctcgtcggtc cgggcgatcc cgaagtccat
     1501 cagcttcacg acgccgttcg gcaggaccat gatgttctgc ggcttgacgt cgcggtggat
     1561 gatccccgcc tcgtggacgg ccgagaggcc ccggcagagc tgcttcgcga tctggaggcc
     1621 cggggcgagg gccacggcgc cccgccggtc gagcacctcg cgcagcgtcg tcccggggac
     1681 gtactccatc gtcaggaagc ggacgccgtc cgcctcgccg aggtcgtgcg tccggacgac
     1741 gttcgggtgc gtgatcttcc tcgcgagccg gatctcctgc ttcagcgtct gggtcgcgtg
     1801 cgtcccctcg gagaacgcct ccggggtgag gaccttcagc gcgacctcct cgtccagctc
     1861 ccggtcgagc acctggtaga ccgatcccat ccccccgcgg ccgaggacgg agacgacgtc
     1921 gtagcgcccg gcgaagagcg ctccgacgcg gggccctctc gccgccgcga gcgcggcgtc
     1981 ctgggggagc gccccggcgg cggtcgccga cgtgggagcg aggttccgcg tcgcgtcggc
     2041 cggtcgccgg cgcatctcgg cgaggagctc ctcgagctga cgcttctcgc gcagctcgcc
     2101 gacgagcgcc tcgaaggccc gggcgaggag cgccacctcg ccgcttccgc cggacgggag
     2161 cttcacctcg agctctccct cccggaccgc gatcgccccg gcggcgagct gctgcagggg
     2221 gcgcgcgatc cggcccccga ggaggaagct gaccgggacg gagacgagga ggacgccgag
     2281 gccgacgagg aggagcgtct cccggatctt ccggaacgcg gccgtctcct cctcgcgtga
     2341 gcgcgagacg acgacggcgc cgagcgtctc gcctccggcc gcgcggagcg ggaccgcctt
     2401 gacgacgcgt cggtcgcccc cgacgaggag gtcgaggggc gcggagtcgg cgtccccctc
     2461 cagaaggcgg gcgagcaccg acgggtcgcg gtcgagcgcc ggcaggaggc tcgcggcccc
     2521 gaacccctcc gtcgcggccg cgacctcgag cttcgtcggc tcccccttct tccccgtctc
     2581 ggcgacgaag gcgacctgcc cccccgtcag gtcgcgcagc tcgcgcgccc gtgtcgcgtc
     2641 gagcgggacc gatgcggcga cgacgccgac gagacgcgct tcgccctttt ccgggtctcc
     2701 ggcgacgacc ggggccgccg cgacgacggc gagcgccgcc ccctcccgga tcgtcgccga
     2761 ggactccgtc cactcctcga gcggcgagga gacccaggcg acctcgcgga acgagcgccg
     2821 ctcctcctcg ctcgggggcc ggtcgctccg ggcgatgagg acaccggccc gatcgaagaa
     2881 gaagaaggtc ctcgcgttca ggatcgcggc cttgtccttc gcggtgtccc agaccgtcgc
     2941 gggcggagag tcgaagatcg ccttgatgcc gggctcctcc gcgacggaac ggagagtcgc
     3001 cttcagctgc gactcgaggc cggcccggta gacggcgacg tcccccggga tcgtccgaag
     3061 cgcctcgcgg atgctctcct cggcgatccg gttcgcgcgc caggtggaga gcgcgaccgc
     3121 ggcgccgagc gtcacgacga cgaggagggc cgcggcgagg aagaactgcg tacgcaggcg
     3181 aaggcctccc ggcctcggga cgctcccgcc gcgaaggggg gggcccgagg cccccgagga
     3241 gccctcgcgc ggggcgacca tcgtcggcac gtcgtcgggc gagggggcgt cagtagcggt
     3301 tctcatcgtc gtccggaggc gggtagtcct tgccgtgctt gttcttgtgg ggcgtcgcct
     3361 tccacttcga ggcgtcgagc gtgacggaga cgtccgtcgt ccctccggcc ggcaccgaag
     3421 cgctcccgct ccactccccg cccctctcgt cccaggcctt caccacatgc cgccccgcgg
     3481 ggaccgcccc gatcgccgcc acgccgtccg cgccggtctg cgcgtagagc tcgccgtcga
     3541 ggacgacgac gtaggccgcc atctgcgggt ggatgttgca gtagacgcgg acgacgcccg
     3601 gctcctcgaa gcgggctgtc cgggccgcgc cgttcctgta gaggccgagg tcgaatcgcc
     3661 ccggctccga caggctgaag acgttgtgga agatcttgtc gaagttcggg aaggagaccg
     3721 cggccccctt cggcacggcg aggacgcgcg gctcgaaccg cttgtcgcgc gaggccatct
     3781 tcggaggcgg cgccgcccgg cggccgcggc ttcccggaag ccagacgacc gcttcgggcg
     3841 cggggagcgc gcgcccgtcg gcgcccgcga cgaggaccct gacgcgcagc gtcccggtcg
     3901 ggatcggggt cggcgtcggc cggggcgtcg cggtcgggcg aggggtcggg ctcggcgccg
     3961 gcgaggggac aggcgccggc ggctcttccg gacgtggccg ggcgctctcg accggctcgc
     4021 cgtctctccc ggctcccggg tccggcgtgc ccggaacgac cggcccgggc gtgccgactg
     4081 cgcccgggtc gacgggcgct gcgccgtccg cctgctcggc cggcgcgggg gtcggcgtcg
     4141 cggtcggagt cggcgtcggc cgccgccgct tccaccacga ccccgtggcc ggggcctgaa
     4201 ggagcaacag ggccgcgagc gcggccacga ggagtctcga cattcggaac ggggcggatt
     4261 ctagtcccgg agcgactcgt tctcggaacg gaggcggcgg acggcggcga cgacctcggc
     4321 cgcggcttcg tcgatctcgc cttcggtcgt gaagcggccc acgccgaagc ggagtgtggc
     4381 cttcccgagc ggctccggca cgccgagggc gcgaaggacg tggctcggct cgccgctcgc
     4441 cgtcgtgcag gccgagccgg aggagaccgc gacgccgcga agggccgcga cgagccgggt
     4501 gccgtcgacc cccgcgaacg agaggctcgc gttgcccggg aggcggtccg gcccggaagg
     4561 cggcccgttc gcggtgacgc cgtcgaggga ggagcggatc gaggcctcga gccggtcccg
     4621 caggcggccg agtcgttcag cttccgccac cctttcccgc cgtgcgattc ggaccgccgc
     4681 cgcgaagccg acgatcccgg ggacgttcag cgtccccgac cggaggcccc gctcgtgccc
     4741 tccgccgtgc aggatcgggg cgagagggac gcgcggccgc gagcggacgt agagggcgcc
     4801 gatccctttc ggtccataga gcttgtgggc gctgagggac gcgagggcga cgcccccgcc
     4861 ggcctcgagc gggaccttcc cgaacgcctg cgccgcgtcc gtgtggaagg cgacgccgcg
     4921 ctccgcgcag agcgcggcga tgcggtcgac cgggttcagg gtcccgacct cgttgttcgc
     4981 ggccatcacg gagacgaggg ccgtccggtc ggtcagcgcc gcctcgacgc tttccggcga
     5041 gacgcgcccc tcccggtcgg gcgggaggac cgtcacctcg aagccctccc gctcgagggc
     5101 gcggcacggg tcgaggacgg cgtggtgctc gacggcggag gtgacgatgt gtcccccgcg
     5161 cccgcgggag gcgcgcgcga cgccgaggat cgcgaggttg ttcgcctcgg tcgcccccga
     5221 cgtgaagacg atctcgcggg gctgggcgcc gagcgcgtcg gcgatctcct cgcgcgcggc
     5281 gttcacggcg ctttcggccg tccagccgaa ggcgtgctgg agggaggcgg cgttgccgaa
     5341 ctcctccgtc agccagggaa gcatcgcctg aaggacgcgc gggtccagcg gcgtcgtcgc
     5401 ctggtggtcc aggtagacgg gccgccggcg cgtcacggcg cggagccggt cgtcccggac
     5461 gcgtccggga cggggacgtg gagcaggtcc tcgagggaga tcgcgtcgaa cgctccgttc
     5521 acgaggtggt tcagccggcg catcgcgtcg gtggcgacgc agaacggacg ggtccggcag
     5581 cgcccgttgg acattcccgc ggcacagtag gtgagcgaga tcggcccctc gagcgcgttg
     5641 acgatcgccc ggagcgtcac ctccgcggcg ggccgggcga gacggtagcc gccgcggctc
     5701 ccccgctcgg acgagaccag gccggcccgg gcgagcttct tcagcacctt ggcgacgatg
     5761 gtggcggaaa cgccgaaagc ggcagcgagc tcggtggtcg aaacgcacgc gttgcggcgc
     5821 gcgatctcgg tcatgacgag gatgccgtag tcgacgatgc ggttgagctt cagcatcgcg
     5881 ggcccccgac cggccactat aggcccggaa aggtgcgggt tcaattcgcg gcaatcgagc
     5941 ggcatgacgc ccgtcacccc ttccggcttc cagaacgtag actcccggcg cgtgcccccg
     6001 ttcctctccc cccttccgcg cgtccgcctc gtcgacgcct tcgcgcggcc gttcgacggc
     6061 gtcgtcgcgg ctgcccggac ctgctacagc ccgaagggga tcgtcaccgt ggacgacgtc
     6121 gcgggggacc ccctcgccga cccggccgag cgcgcgcgcc ggcgcgagag gcgggacgcc
     6181 ctcgcccggg acgtctacgc cgccggccac cacaccacct tccagcacgc ccacctccag
     6241 ttcacgctcg agaacgtcag ccggcagctc gtctggtcgt tcctccacgc ccacccgcac
     6301 tacaactccg agcagacctc ccagcgctac gtcgaggtga agcccggccg ctacgccgtt
     6361 ccggccctcg ccggcgaggc tctcgaggtc taccgcgcct gctgcgagat gcagacggag
     6421 gcctaccgtc ggctcggcga cgccctcgcc ccccgcgccg cgcgcgcctt cttcgcgacg
     6481 ttccggggcc gcgccagcca gcccgagcgc tgggagaggg cgatccgcaa gcgggccctc
     6541 gaggtcgccc ggtatgccct gccggtcgcg acgttcacga cgctctacca cacggttccg
     6601 gccgtcacgc tcctgcgcta ccggcggctc gccgagacgt tcgacgcgcc gaccgagcag
     6661 cggctcgtcg tcgaggcgat gaccgacgag ctcctccggc acgaccccgc ctgggccgcg
     6721 gtcctgcagg agccgatccc gctcgaggag acgccggagc acgcgctcgc cgagcgcctc
     6781 ttctccgcgc cccggtgcga ccgcccgggc gacggctcgt tccgctcccg cttcgacgcc
     6841 tccctcgacg gccgagtctc gaagctcgtc gaccggaagg cgcgcaacga ggagctgctc
     6901 gccgaggcgg tccgggaggt cgtcggcgtc acgcccgaga cgctctccga cgacgacgcc
     6961 atcgccctcg ccgtggaccc cgccgcgaac cggctcctcg gcgagtcgct caacctgacc
     7021 gagcacggga agctctcccg cgcgctccac caccccgcgt acaccttccg gaagaagctc
     7081 tcgcacaccg ccgacagcca ggaccagcgg caccggacga cgcccggctc ccgcccgacg
     7141 ctccacgcct acctctccga cgagcccgac tacgtcaccc cgatgctcct cgaggagccg
     7201 ggagaggccc ggacgctcta cgacgagacg atggcgcgga cgtgggaggc gatcgcacgc
     7261 ctcggcgccc tcggagtgcc ggacgagttc cgggcctacc tcctcccgaa cgccgtcgcg
     7321 ctccgcttca ccgagtcggc cgacctcgcc ggcctgaagc acaagctcgc gatgcgcctc
     7381 tgctggaacg cgcaggagga gatctggcgc gcctcggtcg acgaggccga gcaggtccgt
     7441 gacgccgagc cgcgcatcgg ccggtggctc ctccctccct gcgggctccg tgcccgcgcc
     7501 ggcgcgaagc cggtctgccc cgaaggggaa cgcttctgcg gcgtcctcgt ctggaagcag
     7561 gacctcaccg cctggcgccg cctcctctga ccgccccctc cccggatggg gtcaggtccg
     7621 tcttccgttg ccgggggacg ggggcgtcgc ggatcgcctt ctcgacgtcc gggcccttcg
     7681 cggagcgcgc gagatcgagg agctccttgc ggtctcgcgc cacgtcggcg cccgcggcga
     7741 tcaggctcct caccatcggc aggtcgcctc ttctcaccgc cgagtggagg gccgtccagc
     7801 cgtgggtggt ccgcgcggtg accttcgccc cggcggagag gaggacctcg acgacgtcga
     7861 gcttcccggc ctcggcggcc gcatggagcg ccgtggcgtt cccggccccc ttccgcgtga
     7921 cgtcggctcc tcccgcaagg agcgcgcgaa cgacgtcggc cccctgcccc tcggccgccg
     7981 cgatgaggag cggcctcccg gcatagccca cgccgttcgg gtccgcgccc gcggcgagga
     8041 ggactcggac gacctccgcg tgcccccgct cgaccgccga ctcgaggacg ctccctcccg
     8101 gctcgcccgt cgtcgccggc atccccgcgg cgaggaagag cctcacggcc tcgatgtccc
     8161 catcgcggac gacccggtcg agggcgctcg gggtgagctc gagaccccgc tcctcgagag
     8221 tggccttcgc ctgctcttcg gaaggaaggc ccggatcggt ggtccgcgtc ggcttcgccg
     8281 ccggccggaa gagcggcgcg tcgaacgtcg cgtcgaacgc gaacgagaat cccgggtcgt
     8341 cgaagacgat ccgacccgcg agccggccgg ccgcagcagg ggccggcacg agcctcgcga
     8401 cctcgcgcac ctcgaggccc gccggcagct cgtcgtggtg gaagaagacg gccggagcgc
     8461 cgccgcccgc gccgaggacg acctcgacgc cgcggaaccc gaacgacggc agccgcctca
     8521 ggagctcctc gaggacctcc agcggcaccg gctcctccga gaggacgagc cgcgtctcgt
     8581 cggccgccgc gttccgcggc cgcacgacga cggcgtaggc gtggcgcagc ggcgtcttcg
     8641 ctccctgggc cttcatctcg ccgctcgccg tcccggtcca cgtcgccggc tgggcggcgg
     8701 gaagggaccc gcccccggac cccatcgcca ctgcgctcga cgcggccggc gccgccgccg
     8761 cgagggcgag gaggagggcg gccgcggccc gcgcaggaga tcgcctcctc gccggcgggc
     8821 ggctcgcgtc cggccccctt ctcacttcgc ccccgccgcc ttcagcatcg ccacgacgtc
     8881 gtcctggtct cccttccggg cctgttgaag cggcgtcccg ctgtactcgg ccttcgcgtt
     8941 cgggtttgcc ttcagctcga ggaggagctg gacgatgtcg gggaaaccgc tggcgatcgc
     9001 gacatggagc gccgtctcgc cgacgttcac cttcgtcggg gcctggttca ccttcgcccc
     9061 cttcgcgacg agcaggcgga tcgcttccgg cttcctcccc atcacggcgt gcttgagcgg
     9121 cgtgtagccg atgtcgtcga cgacgttcac gtccccgccc gcggcgacga gcacctcgag
     9181 cgtctcgagg ttcccctcgt aggccggcag gaagaggacc gtccgcccgt ccgtcagctt
     9241 ctcgttgacc ttcgctccct ttgagatcag gtagcggacg atctccaggt cctggccgac
     9301 gacctcctcg agcgggctct tgacgaacca gtggacctcc ccccggtcgt tgaccctccg
     9361 gtccttctcc aggccgttca cgtcggcgcc cgcctcgacg aggagccgga cgatctccat
     9421 gttcttccgc cagcaggcct tcacgagggg gttcgacgcg accgtgtcgt gcacgatcgg
     9481 ggtgttcgcc gagtgcccgg ccgcgaggag ctcctttacg gcatcgacgt cctgtcggtc
     9541 gatcgccacg tcgaagaggt gccccggtga cgactcatcc tcccgggccg cggcggggac
     9601 ggcgagggtc agaagagaga gagcgagaac gcatggcccg gcgcggttca tacggcacct
     9661 ccgaaacgtg attcgtttcg acagacccta tcaccggttc gatcgcgcga tccttcgggc
     9721 ccgtgcgcgg ccgcgccccg cgcgtcgcgc ctcagggcgc ttcgagcgcc tcctcgatcc
     9781 gtcgaatcgc ctccgacgcg gacgcggcct ccggcacgag gacgaccggg aggcagcccg
     9841 gcacctcctc gcggatcgcg gtccggacgg ccgcgtcgtc cgtcacgacg gcgtcgacgc
     9901 gcgccgagcg cgcgagccgc tcgagcgtgg cgtcgacgcc ccgggccgtc aggaggacgg
     9961 aggaaggagg gaggagcgcg cggaggtcgt ccgcgagccg gtcccgccgc gaggagacga
    10021 ggaggtgccg cacggctccg tcactccgcg acgaggagcg gcgcggcgac cgggaagagg
    10081 ggcttcccgc tccggatccc ctgtcccgcg tagacgacta tgtagtagtg cccggggcca
    10141 cggtcgagcg ggatcgtcag ctcgatccgc cccgcggcat tcgcccggaa gtcgcccgac
    10201 tcgcccgact gccagcgcgt cccggccggc gggtaaggcc gaagcgtccg ccacggaggc
    10261 ggcagcccgt acgacgcgcg ggccgcgatc tcggcgcgcg acatcggatt cgggacgggc
    10321 gcgaaggcca cctcgatcgc cccgacgctc cagcccttcg ggagcttcag cgcgagcggg
    10381 gcgccgcccc ccgccctcac ggggcccgcc ggcagctcgg cccattcgag gacgcgccgg
    10441 ctgtactcct ccgtcatccg catctcgccg ccgacgagcg cgaagccgat tccgacgtgc
    10501 gtccagatcg ggtcgaggat ggtccggcgg tgtccgtcgt tcggcggctc ctccgccatg
    10561 aaggccgcgt gcatccggag gaggagctcg gccgggctct ccgtgaagtc gaagccggtc
    10621 cggctctccg ccgcgaagtt ctgggcgtgg tggtcgatcc cgccggcgag ggcccagcgc
    10681 aggtacgggg cccgaccggc gaggtcccag tggcccgtcg tccggtcccg cgcggcctgc
    10741 tcgcagaact cgtcgcccac cttcgccccg aggaggtcgt aggcgagagg aggcgcgccg
    10801 tgctcggcgc gctcgcggtt gatgacgcgc aggagctccg ccttcgcggc cgggacccgc
    10861 tcgacgtagg ccggcgcgag gcccggcagg acgacgcgga ggggcggcgc gttccgggcc
    10921 ccgtccagcg gcagggccgc gacggccagc agcgccgcgc cgagggcgac tccccctacg
    10981 ggcccggccg ctcccgcgcg gcgtttcgat tccggacgga cctctttcac ccgtagaatg
    11041 ttacgggcac gaaggcgtgc ggttgcggcg ttccgggaac ggacaccgcc cggaaggatc
    11101 gtccccgacg gcccggccgc cgggatcttc cgccgcgttc gccaaagacc cggccccgcc
    11161 gccccgaggg cggcaggaag gaacgagcat gtacgccatc gaagagatcc acgcccggga
    11221 gatcctggat tcgcgcggca acccgacgat cgaggtcgag tgcctcctcg aggggggcgc
    11281 gtcgggccgc gccggcgtcc cgtcgggcgc ctcgaccggg acccgcgaag cgctcgagct
    11341 gcgcgacggc gacccgaagc gctacggcgg caaggggacg ctcaaggccg tcgacaacgt
    11401 caacaagacg atcgctcccg agcttctcgg cttcgacgcc cgcgaccagg cgggcctcga
    11461 ccgcctcatg tgcgatctcg acggcacgga gttcaagtcg aagctcggcg cgaacgcgac
    11521 gctcggcgtc tcgatggcgg tctgccgcgc cgcggccgag gggtgcgacc tcccgctcta
    11581 ccgctacctc ggcgggacgg gcgcggtctc gctcccggtg ccgatgatga acgtcgtcaa
    11641 cggcggcgcc cacgccgaca acccgctcga ccaccaggag ttcatgctcg tccccgcggg
    11701 cttcccctcg ttccgcgagg cgctccgcgc cggggccgag acgttccacg cgctgaagtc
    11761 gatcctgaag aagaaggggc tcgcgacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnntcg tctcgatcga ggacggctgc gccgagggcg acaaggccgg ctggaagctg
    11941 atgaccgaga agctcggcgc gaagatccat ctcatcggcg acgacctctt cgtcacgaac
    12001 ccgaagatcc tcgccgaagg gatccgggac ggcatcgcga acggcctcct cgtgaaggtg
    12061 aaccagatcg gcacgatcac cgagacgctc gaggcggtgc ggatggcgca ggtcgcccgc
    12121 tacgtcaccg ccgtctccca ccgctccggc gagaccgagg acacgacgat cgccgacctc
    12181 gccgtcgcga cgagctcgat gttcatcaag accggctccg cgtcccgctc cgagcggctc
    12241 gcgaagtaca accgcctcct ccggatcgag gaggagctcg ccgacgccgg ccgcttccac
    12301 ggcctggccg tgaagaagtg accgcgtgag gaaggcctga ggagcagcga acggcgttga
    12361 ggttcgagcg gatcaccggc ccgaagaagg gcctcgtctc gcggacgggg gggcggcccg
    12421 tgctgccctc ccgccccgcg cgccgtctcg acgagccgtc cgcctccggc cccgccgccc
    12481 ggcccgaccg gccccacgcc cacccgctct cctacgtcgg cttctccctc ctcctctcgt
    12541 tccttctctt cgtcttcttt ctggccggcg accgcggctt cctccaggtg aggaagcaga
    12601 aggcgcaggt cgccgcgctc caggcggagg tctccgccat cgcggggagg aacgaggcgc
    12661 tcgagaagga gatcgagagg ctggcgaagg acccggcggc cgtggagagg atcgcccggg
    12721 aagacctcca gctcgttgcg cccggcgacg tcgtcctcgt cctgccgccc ggcttcgagg
    12781 agaaggtcac gccgacccgg ccgcccgccg gcgtcgccgc ccgctgagga gcgcccgacc
    12841 tcctttcgcg cgtccggcat gacgcccgtc atatcggcca cggaactccc cccgcctcct
    12901 tcccgtatcc ggccccgaac gaaacgagcc cggtgctccc ggggccgctc ccgaccggag
    12961 cggaggaggt cgatccatgt cctggttcct gcagttcgcc catctcctcg ccaccgccgc
    13021 ctggatcggc gggatgatct tcatgaaggc gatcctcgtc ccgagcctcg ccgtcctcga
    13081 gccgccgcag cgcggacggc tcatggggag cgtcgcgaag cggttcaccg cgctcgcctg
    13141 gacctcgacg ctcctcctcg ccgtgaccgg tctcctgaag tcccccgagg ggctcctcgg
    13201 cgacacggcg acggacgtcg ggctcctcct caccgtcaag gtcgcgctct tcggcgtgat
    13261 ggtcgtcgtc ggcacggtca tcacgttcgc ggtcgcgccg aagctcgtcg ccctcgctcc
    13321 cgctcccggc tccacgccaa gccccgcctt cgtgacggcg cagaaggcgc tggacgcgct
    13381 ctccggggcc aacgcgctcc tggggctcgt gatcgtcggg atcgtctcgg cgttctgagg
    13441 cgccgcggcg cgggaggccg cgcaggccgc ctccccgccg ctcccgcgcg cgccgcttct
    13501 ctgagacgat cggcgcgtga tctcacgcgc cgacgccgcg gccctcgacg ccgccgaccc
    13561 gctcgcgtcc ttccgcgacc gcttcgtcct ccccgagggc ctcgtctatc tcgacggcaa
    13621 ctccctcggc gcgctgccga agggggccgc cgaacgcatg gtcgaggtcg tctctcgcga
    13681 atggggcgcg ggactgatcc ggagctggaa cgacgccggg tggatcgacc tgccgcgccg
    13741 cgtgggagac aagatcgcgc gtctcgtggg cggcgagccg ggagaggtcg tctgcaccga
    13801 ctcgacgtcg gtcaacctgt ggaaggtcct cgccgcggcc ctctcgctcc gcccggagcg
    13861 gcggacggtc ctctccgagg aggggaactt cccgaccgac ctctacgtcg ccgaggggct
    13921 cgcgcgcctc ctcgggcagg ggcatcggct ccgcctcgtt ccgaaggggg agctcctcac
    13981 gtctctcgac gccgacgtcg ccgccctcct cctgacgcac gtcgactacc ggaccggcgt
    14041 gcgacacgac atggagcggc ttacgcgcgc cgcgcacgag gccggcgcga tcgcgatctg
    14101 ggacctcgcc cactcggccg gcgcgatgcc ggtcgacctc cgcgccgccg gcgccgacct
    14161 cgccgtcggc tgcggctaca agtacctctg cggaggaccc ggcgccccgg cgttcctctg
    14221 ggtcccgaag cgcctccagg actccttcgt ccagccgctc tccggctggc tcggccacgc
    14281 cgagccgttc gcgttcgaga gggtctacag gcccgccgag ggtgtcggcc gcttcctctg
    14341 cggcacgccg ccgatcctct cgatgtcgtc gctcgacgcc gcgctcgacg tgatcctcga
    14401 ggccgacctc gcgatcgtcc gcgcgaagtc ggaggcgctc acctcgttct tcatcgaggc
    14461 ggtggagcag gagtgcggcg acgccgtgcg gctcgtctcc ccgcgcgtcc cttccctccg
    14521 cggcagccag gtctccttca cgcaccccga cgcctacgcg gtgatgcggg cgctcatcga
    14581 gcgaagcgtc gtcggcgact tccgcgcccc cgacgtcctc cgcttcggct tcgcgccgct
    14641 ctacgtcggc ttcgtcgacg cctgggacgc cgcgaaggcg ctcgcggacg tgctctcgtc
    14701 gcacgcccac gagcgccccg agctccgccg ccgcgcggcg gtgacctgac ctaccggtag
    14761 cgggctcgcg tccagtagcc cgggacccag cggccccagc gatcgtagta gccgttcacc
    14821 cagaccgcgt ccaggaaggg cttcctcacc cagcgcccct cgacgaacgc ccagcggtcg
    14881 cgatcgcggt agcgatggtc gttccgccac ccgtcgtcgc ggtagcggtc gcgatggcga
    14941 cggtcctcgt cgcgccagcg gtctcggcgg tcccggtggt cgcgccggtc gacgcggacg
    15001 tcgacgtgcg gcggcggcgg gagaggcgcg tgacgaagga cctcgtgcgg aggcggcggc
    15061 agcgggaggt tcacgtggat ccgcaggtcc gccagggccg acggcgccac ggcgagtgcc
    15121 agggccgccg tcgcggcgag gaggatgttc ctgtcggcgt tcttcatgtc ggacctcctg
    15181 ggacgttctc gggagcccgt cgctcccgtc ctcgtcattg caggggcgat gccagccgtc
    15241 cgcgccccgg gaggtgtgcg ccggatcacc gcgagagacg aaaaggcccc ccgcgacgtc
    15301 gcgaggggcc ttctcccggc ctctccggag gacggaggtg ccgcgttcgt cgcgttttct
    15361 cctacgccga gtggatccgg tcctcgaacg ccgcgagagc cgccttcgcc ccttcgccca
    15421 tggcgatgac gatctgcttc cacggcacgg tcgtgacgtc gcccgccgcg tagatcccct
    15481 tcgtcgacgt ccggcacttc tcgtcgacga cgatctcgcc gaaacggttc acgtcgacga
    15541 ggccgcggac gaggcccgag ttcggggcga ggccgatctg gatgaagatg ccgtcgatcg
    15601 ggaacgtcct ctcggcgtcg gtcgcgcggt cgacgacctt gatcgccgtc accttgctcc
    15661 cgtcgccgag gatctccgcc gtccggtggt tcacgatcac ctcggtgttc gggagcttcc
    15721 tcaggttgtc gacgaggacc ttgtcggcct tcagctcgtt catgaactcg acgaccgtca
    15781 cccgcgagca gatcccggcg aggtcgatgg ccgcctcgat gcccgagttg ccgccgccga
    15841 tgacgacgac ctcccggtcc ttgtagaacg gaccgtcgca gtgcgggcag aacgcgacgc
    15901 cgcgcccgat gtactccttc tcgcccggga cgttcagctc gcgccactgc gctcccgtcg
    15961 ccatgacgag cgccttcgtg cggaggacct cgccgccggc gaggcggacg gtcttcagct
    16021 ctccgtcctc gatcgcctcc acgcggcggt tgtcgaggac ctcgaccggg tagctccgca
    16081 ggtgggcgag gaggtcggcc gcgagcctct tcccctcggt gcgcgtcgtc ccgatcaggt
    16141 tctcgatccc gagcgtctcg aggacctgcc cgccgacgtt cttcgcgacg agcgccgtcc
    16201 ggagcccctt gcggaccgag tagatcgccg cggacgcgcc cgcgggcccg ccgccgacga
    16261 cgaggacgtc gtacggatcg cgcaccggga ggtcctgcgc cgcgtccgac ggcgcggaac
    16321 cgaagcgctc ctcgagcgcg tcgaggagct ccgcgaggcc ggcccggccg acggagacct
    16381 gcgcgttctc gtggccgagc gagatgacgg tcgggacgcc ctggatgccg agcttctcga
    16441 cctccccttc cgcgaggccg ccgtcgacca tctcgtggac gaagtcgggg tggatcaccg
    16501 ccatcaggtt cagcgcctgc accacctcgg ggcagttgtg acacgacaga gagacgtacg
    16561 tccggaggcg gaccggcccc ttcaggtgcc ggatccggct ctggattccc tcgtcgggcc
    16621 gcttcccctg ccccgcggcg tacttcaccg cgaggacgag cgacgagaac tcgtgcccgc
    16681 ccgggatgcc gcggaagagg atcgtcgccg cgcgcccctc cgccgccacc tcgaacgccg
    16741 ggatcgcgct cgcctcccct tccacgcggt ggacgatctt ccccggcgcg gtcgcggcca
    16801 cgtccgcgag catcgtcgtg agctccccgt tcttcgggtg ctcggacgga cgctggcgga
    16861 ggacgaccgt cacggggagg tcgctgtaga tctgctggag ctgcgcggag agagaagcgt
    16921 cgagcatcgt tgtcctcttc gggggcgtgc gggagtcgag gcgggaagga aggccgtccg
    16981 gcgcgtggtc acgcacctcg agcggccgga gggggacccg gcgttcccgc cggatccccc
    17041 gtggaccggg acgtgaatca gatcttgccg acgaggtcga gcgagggctt gagcgtctcc
    17101 tggccggggg tccacttggc cgggcaaacc tcgttcgggt ggctcgcgac gtactgcgcc
    17161 gcctgcacct tccggaggag ctccttggcg tcgcggccga tgccgttgtc gtggatctcg
    17221 gcgatcttga cctgcccctc ggggttcacg acgaacgtgc cgcggtaggc gagacccgcc
    17281 tcgtcgatgt ggacgccgaa gcccttcgag aggatgtggg tcgggtcggc gagcatgacg
    17341 tactggatct tcttgatcgt gtcggaggcg tcgtgccacg ccttgtgcgt gaagtgggtg
    17401 tcggtcgaga cggagtagat ctcgacgccc atcttcttga actcggcgta gttgtcggcc
    17461 aggtcgccga gctcggtcgg gcagacgaac gtgaagtcgg ccgggtagaa gaagaag
//
LOCUS       DAOTEZ010000028        17403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2204_length_17403_cov_6.078049, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000028 DAOTEZ010000000
VERSION     DAOTEZ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17403
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2204_length_17403_cov_6.078049"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="P5164_02535"
     CDS             complement(<1..907)
                     /locus_tag="P5164_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42800.1"
                     /translation="MDEFDPKELVEANRPAVEATVVEELPEGDDDAEALDEGDAESEA
                     ASPEGGAVPGEPSRKRRRRRRRRGRSKGTGAENGPGGAEAAGEGAPRREPAVQAAPPV
                     AVRGLVKLHGDHSGLVVSNETFFAEKGDPFLPKYLVEAEDLEDGLLIEAEAVARGPKG
                     PMVQKILSIEGMTPAEYRSKYVQFHKGISIDPNRRLRMETATDENSGRVLDLVVPIGR
                     GQRCLIVAPPKAGKTFLLKTMANAIAKNDPDVIIFMLLVDERPEEVTDMQRGITGQVV
                     ASSADMNAAHHIAVAEATFERAKRLV"
     gene            complement(1147..2277)
                     /gene="amrS"
                     /locus_tag="P5164_02540"
     CDS             complement(1147..2277)
                     /gene="amrS"
                     /locus_tag="P5164_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HRY42801.1"
                     /translation="MAPADSVREHLAGLTAKATLVREESGKLRCLACGHRCLLAEGGQ
                     GVCRVRFVRDGELRAPWGYVASVAADPIEKKPFFHVLPGSTALSFGMLGCDLRCPYCQ
                     NWEISQTLRDPNADDWAHPRPATPEGIVAAALEAGAQVVTSTYNEPLITAEWAAAVFA
                     EARRAGLLTSFVSNGHATPDVLDFLRPLVDAYKVDLKGMREGAYREVGGRLSVVLDAL
                     AGLVARGFWVEVVTLLVPGRNDSDEELRDAARFLATLSPDLPWHVTAFHPDYRMGDVR
                     ATPAATLLRAVDIGRREGLRYVYAGNLPGGVGDAETTACPGCSERLVERRGFRVVARR
                     IGADGRCPRCERLIPGIWSRGGRISPETPGAASVARTRSSTA"
     gene            2348..4975
                     /locus_tag="P5164_02545"
     CDS             2348..4975
                     /locus_tag="P5164_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42802.1"
                     /translation="MTRLFRMAALAAGVAATALPLFAQTPTPTPTPTATPTPVPPTSI
                     EASVASLGIPNGGRYDASNMILEADGTIWTASANENVLARISADEKKVRKWTMPRDAA
                     PSHLLKEPDGKLWIAQLGGFQVSLFDPQTSELTEWADGARRPTAFVRKGDGTLWLPET
                     NGTLANFDPATGTFVYWRSTDAANPISSLTYPFLDTDGSLWSADFLRGAILRFAPDGS
                     KVTRWALPDATAQPSKIIRGPDGHLWFSFFGAAQLARFDPATRELKRYAVGVFSQPFD
                     MKVYKDRIVYTEQLIGEVGVFDPLASTPAATDTLEGKEIVLTRTTTTVPPTKKVLETI
                     ELDVAPADPAAVTGSGLPGLARYTAVSGVPYALVLDETRKRFLVGATAEIAAVLPPVP
                     ATVDDHFFPAASSIGGRGGVRWATQVVTWNRGSADTAGVRVDATVNERLLPTDWIIGI
                     SPTTNLTIGPGKIVSQSDPIGVEMKGPDTSGGLRLTSGATATRFGDVHSWIRVYRTRD
                     DGGTYGFARNYVKGAEALAAGETGFLFTPPDAGSRVNAGLLVIEAAEGTVSIVDAAGA
                     PLSPPFAFRWPAGYHEQASTIFSAFGIPPAPSARIVYTVTKGRVLPFGTSIDAVSSDP
                     VDLPFFGPRGTAANQWVFGVERGGGPLGPTSRTDLQLFNGAAGDSSVTLVLRASRLAA
                     SPGAAPPARAVSLTVPKGKVVALNDVVRETFGIDVFAGSLDVVSDPPVHAFARVTAAD
                     ASGGRHGYGMPALRADSTAAAGSRAVFIQASDAGWDVMESELQLANPTDSAGEVTLRA
                     FDTEGAAAGEPVTLTLGPKESVRVPAAFFTLSGFGAPIGRIDVAPVAGSQPVFALLVR
                     RDQKTGDADAVVPYVVPAT"
     gene            complement(5053..6723)
                     /locus_tag="P5164_02550"
     CDS             complement(5053..6723)
                     /locus_tag="P5164_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HRY42803.1"
                     /translation="MSSRRPFLLLLAFVLALPALAAERVTITVLQTSDLHANLLPWNY
                     ARGEAADWGLARVATRVREIRKSSKHVLLLDGGDTIQGAPAGWLEARRPTGGPHFMAV
                     AMNALRYDAMAVGNHEFNFGLEVLRRAQKDSSFPWLSANTRNAAGGGPAFPEYVVKTF
                     GRVRVGVLGLTTPNIPGWEPEANRPGLEWENPVETAARLVPLLRQKERCDVVVVLFHS
                     GLEVDPDTGEANGTDYENHVVRLAKAVPGIDLILTGHSHRRVPLTRVHGVPVTQPGRW
                     GEVLARVDLVVERGKGTARVVEARGELLPSDESVAVDPEVATIARPHHERALAYLGEP
                     IAAATGPFPADRARLEDSALLDLINETQLAATGAQLSMTSLLPFRHDGWKEGPVTVRD
                     VYSVYPFENQLVVVEIDGARLREILEHAATFYESAEWKDGRLLLTPRAGMRPYNFDVV
                     QGASYRIDPTAPAGRRVRDLRYRGREVKDTDVFTLAVNSYRAQGSGGYAALRGARVVR
                     AYNDEVRELLVERLRNARTIDPVTDRNWVLAPETTWAPPAAGGAPAPR"
     gene            6800..7528
                     /gene="nth"
                     /locus_tag="P5164_02555"
     CDS             6800..7528
                     /gene="nth"
                     /locus_tag="P5164_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013615095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRY42804.1"
                     /translation="MPARPKPKPAPAPEVLARLRAAFPGADCSLDHADAFQLLVSTIL
                     SAQCTDARVNAVTPALFRRLPTPAAMAAAGPELEELIRSTGFFNAKARSLRGACRAIV
                     EEHGGTVPRTMEALNALPGVGRKTANVVLGNAFGSPDGVVVDTHVGRLARRLGWSGAT
                     DPEKVERDLNALLPHAEWVFAGHALILHGRRTCTSRAPRCSGCLLEEICPKVGVAPAA
                     KPGAGRRAAPEARTPDAARAGRRR"
     gene            7528..8121
                     /locus_tag="P5164_02560"
     CDS             7528..8121
                     /locus_tag="P5164_02560"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019941783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HRY42805.1"
                     /translation="MLPGSGSIEVICGSMFSGKSEELIRRVTRAKIARRRVQVFKPKL
                     DDRFSEVEVVSHTGMRLTALSVASSAEVLARTDDRTEVVGIDEAQFFDAGIVEVASRL
                     ADLGKRVVVAGLDQDYLGRPFEPMPTLMALAEEVVKTRAICILCGGPASRTQRLVASK
                     ERVVVGAAGMYEARCRRCFDPGVVEVDGVLPFPEESS"
     gene            complement(8478..10064)
                     /locus_tag="P5164_02565"
     CDS             complement(8478..10064)
                     /locus_tag="P5164_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013988369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRY42806.1"
                     /translation="MSTNKPDTATTSGIPVRPSYAPGDVPQGWDPGVPGAFPYTRGAM
                     AGGYRKKLWTMRQYAGFSTARESNARYRYLLSQGQTGLSVAFDLPTQIGFDSDAALAR
                     GEVGRVGVAIDSIEDMETLLDGIPLGDVSISMTINATASVLLGFLLAVARRRGTEWKA
                     LSGTIQNDILKEYAARGTYAFPPKPSMRLITDIFGFCAENVPRWNTISISGYHIREAG
                     STAVQEVAFTLADGVEYLSAAVRAGLDAKAIAPRLTFFFNVHNDFLEEIAKFRAARTL
                     WARLLKDRFGIDDPRAQALRFHTQTAGSTLTAQQPDVNVVRVAIQALAAVLGGTQSLH
                     TNSRDEALALPTTEAARLALRTQQVIAYETGVARVADPLGGSWAIEALTAEIVEKASA
                     LIGRIDEMGGALAAIEAGYFQREIQEAAYQAQKAIESKSQVVVGVNEFVLEEENPIPT
                     MTIDPKVETEQVERLAAFRARRDDAKVERLRADLVAAAKEGRNLMPPIVEAVDGNVTL
                     GEVVASLKTVFGEHRDAVTL"
     gene            10234..11670
                     /locus_tag="P5164_02570"
     CDS             10234..11670
                     /locus_tag="P5164_02570"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HRY42807.1"
                     /translation="MSKSLSGTLDTFALADLLQWIEINRLSGRLTVSRGEDRRMIDVK
                     NGAIVFVSSVRPEERLGTFLVARGILPEPVVYELLADSFLTGRSLTRLVFETGLLSRD
                     ALAGAVEELALKVLFDLFHWQGARFVYDPTVLTEDLLRIQLSLRGQLLALEGARAVDD
                     TARRRTAESVEAEEPASNAEELSPRNVARSFFAVVEGLDMESPPPSVWQERFELFGRF
                     SEKVAASLRSPFRPFPLFEDTAERCRAALESGAETEETFRLAATDPFLTVDLLFLGNA
                     LRTPATGLLSTVDGALAAIGTGALRRFLELLVSPGAPVVSGRDRLERALRSAAVSTAV
                     AASHVAPSLGEDPGRAWVLALLEPVGGYELLKLLLTEDFDPGPFRAATLGWYRAASGR
                     ALARKLNLPDAFADVLGSDGQVSSRSSVAEQLVFFAKQMVAGEQVGREWTSDDPELAD
                     RYAALAVDDALADRIGRDASALRELLGL"
     gene            11743..13377
                     /locus_tag="P5164_02575"
     CDS             11743..13377
                     /locus_tag="P5164_02575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRY42808.1"
                     /translation="MVARNAASAILVLSLAFAPGSASLAAEPPETALPAGVRAAVEVR
                     ALDLDVVATKDGRPVTDLTRDEFVVRVDKKVVPIDYFARVEAGTVHGPDLATASPDLV
                     LDTLRNDAGETYVPRQFLVYFDDERLLPQDRKPVLEGLRDFMTRLSPSDRASIVSYNG
                     ATRVLVPFTSSKEDLLDGLSKLETLAPRGLALESEYQRTVREIRISPRSRASLIRSWS
                     TEAAHRERWALRELARSVSALAARSGKRTLLLVTNGYEWHPGRALALAYGPSLVSQFA
                     EDVGEEIEKVLAEANAAGITIQVFDAKGLGAGVDASERSNPAISPFYRAQGYRDSMVT
                     LATGTGGSLVENRNEFRSDLDRVYREASGYYSVGVTLSGLAGTGERKVEVRTTRPGVV
                     VRTRNGFLPRSADDAAVDRVEMALLTPGAPGDFPATLRIGSLSPAGGLARRTGPYEVQ
                     FSLEELTFREEGDRKTAVVDVTLAAIQDTGARSDVKPERKMISIPASEWERVRAEPFV
                     YRGQVKSGKGNIRFVAGVRDVATGRMALASADLRVE"
     gene            complement(13467..15083)
                     /locus_tag="P5164_02580"
     CDS             complement(13467..15083)
                     /locus_tag="P5164_02580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HRY42809.1"
                     /translation="MRKLRLLATLVPLVALALPAAAQLDKFKDWESSPEFVYYATDDE
                     KKAWTGVTNDEEAQKFFNLFWGKRHPDYQKTAQNVFRTRFDALVAEADKRFTLGKKRG
                     ALTERGKVLILLGPPKAIASKLESAGAGAAGGEGEEGSFLQTGGNVVVIQFQYDKEQL
                     PEWAGMKSFRVNFTVDERAERESADKSSDIKKLWKKAIAAAIVNPKMTEPPVYKTREE
                     YEAEMKAAAEAAAEAAKGPVLSAPVRASLEALLGKEPQGSLTLFPLAYGDKATRLMVQ
                     LYVPASAVPAGAAGAAPEGMKIALLVRGKDGKDAARREEPAVLEKVREDWFVDVALPV
                     DAGEYQVAALLLDASGAEKVSAYRPVTVIPLPSELAMSPLLLACTDIAADGAKPEEPF
                     VFSVRKFVARGGDTLEKTDGLAYVARVYNPAVDPATKRLHLKRSISIKPKNGSTIDVP
                     QPPDEPMVVPEQAGSATALVIDLAGSIVDVNLGDYFRPGEYVMTLKITDVISGKSVEA
                     KTPFSLAAPPAPAKAPAAKPAAPKAPAPKK"
     gene            complement(15181..16311)
                     /locus_tag="P5164_02585"
     CDS             complement(15181..16311)
                     /locus_tag="P5164_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY42810.1"
                     /translation="MIRPLVRPWRSGAVLVLAFGVFAGGCRTTEETPVLRLTPERWRE
                     VVAARGVDPGEVPMPMMVTPQIRAAAEHLAGPAGDDGERLRRLQAAILDRKSYAFEYD
                     TVATFTAAEAFAFRRGNCVSFTNLFIAFGRALGIPLQAALVFRRARSEKEGDLVMVYN
                     HMVAVLPKLRHLTVYDFYMTRDESRVDLKLIDDLAVAAISASNRGVEALRAGDFSRAR
                     TLLERATKLQRSLPDLHSNLGIVRWRQGDIEGAYQAFREGLDLDPHRPALLHNLAALY
                     IEQGRSTEARAALAAANTRDASAYLFVVQGDLELAGGNFKEARTAYRRAYREGKNLFE
                     ALLGLARVERALGNEAAAQRALRKALKIRPDDPQVQALLGSS"
     gene            16512..>17403
                     /gene="argS"
                     /locus_tag="P5164_02590"
     CDS             16512..>17403
                     /gene="argS"
                     /locus_tag="P5164_02590"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF015450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRY42811.1"
                     /translation="MIPGLKNRVEAAVKDACRRLWDVEPPRLVLETPPKVELGDLALP
                     IAFELAKVLRRPPRKIAEELAPALELPPIVARRSVEGSGYVNLFLDRPRALAAMLTPP
                     PEAEAVPGKIIVEHTNINPNKAAHIGHLRNAVLGDVLVHLFRRLGKRVEVQNYLDDTG
                     VQVADVVVGLEHPDLLAEPGRAEELGAEIREILSAFPDDAPGTEPRRLGDVTWDLYAL
                     VTRSYAADPSLEERRKATLHAIEEAALGHEMTDEGRATAALASRLAEAVVRCHLKTME
                     RIGVSYDLLPRESDILGRRFW"
BASE COUNT         2341 a         6390 c         6110 g         2562 t
ORIGIN      
        1 cgacgaggcg cttggcccgc tcgaacgtcg cctcggcgac ggcgatgtgg tgcgcggcgt
       61 tcatgtcggc ggaggaggcg acgacctggc cggtgatgcc gcgctgcatg tccgtgacct
      121 cttccggccg ctcgtcgacg aggagcatga agatgatcac gtcggggtcg ttcttcgcga
      181 tggcgttcgc catcgtcttc aggaggaacg tcttcccggc cttcggcggc gcgacgatca
      241 ggcagcgctg tccgcggccg atcgggacga cgaggtcgag gacgcgcccc gagttctcgt
      301 ccgtcgccgt ttccatccgg aggcgccggt tcgggtcgat cgagatcccc ttgtggaact
      361 ggacgtactt cgagcggtac tcggcgggcg tcatcccttc gatcgagagg atcttctgga
      421 ccatcggccc cttcggcccc cgcgcgaccg cctcggcctc gatcaggaga ccgtcctcga
      481 ggtcctccgc ctcgaccagg tacttcggga ggaaggggtc ccccttctcc gcgaagaacg
      541 tctcgttcga gacgacgagg ccggagtgat cgccgtgaag cttcacgagc ccgcgaacgg
      601 cgacaggggg ggctgcctgg acggccggct cgcggcgcgg cgccccctcc ccggccgcct
      661 cggccccgcc ggggccgttc tccgcgccgg tccccttgct gcggccccgg cgccgccggc
      721 ggcgacggcg cttgcgcgac ggctcgccgg gcacggcgcc gccttcgggc gatgcggcct
      781 cggattcggc gtcgccctcg tcgagggcct cggcgtcgtc gtccccctcg gggagctcct
      841 cgacgaccgt ggcttcgacg gcaggccgat tcgcttctac gagctctttc gggtcgaatt
      901 cgtccaagct gacctccgga acgggcgttt cgcggccgtt ccgtttcctg cgggcccccc
      961 acgcgagcgc tcagcccggg cggacggcat ttcctactcg gtccgaaggg ggagggcggg
     1021 tcgtgatcct cgcgactccc tcgcccggcg ccccgttcac gccacccgct tccgcagggc
     1081 cgcgccgcgc cgggccagac gccggaatgc tatgccggaa cggaaccggc cgtcaattcg
     1141 gcgctctcag gccgtcgagg aacgcgtccg ggcgacggaa gcggccccgg gcgtctccgg
     1201 actgatccgt ccgccccgcg accagatccc cgggatgagc cgctcgcacc gcgggcacct
     1261 tccgtcggcg ccgatccgac gcgccacgac ccggaacccc cgcctctcga cgagccgctc
     1321 ggagcagccg gggcaggccg tcgtctccgc gtctcccacg ccccccggaa ggttccccgc
     1381 gtagacgtac cgaaggccct ctcggcggcc gatgtcgacg gcgcgaagga gcgtcgcggc
     1441 gggcgtagcc cggacgtcgc ccatcctgta gtcgggatgg aacgcggtga cgtgccaggg
     1501 cagatcggga gagagcgtcg cgaggaaccg ggccgcgtcg cggagctctt cgtccgagtc
     1561 gtttctcccc ggcacgagga gcgtgaccac ctccacccag aagcctcgcg ccacgagccc
     1621 cgccagcgcg tcgaggacga ccgacaggcg cccgccgacc tcccggtagg cgccctcccg
     1681 catccccttc aggtcgacct tgtaggcgtc gacgaggggc cggaggaagt ccagcacatc
     1741 gggcgtcgcg tgcccgttcg agacgaagct cgtcaggagg cccgcccggc gggcctcggc
     1801 gaagacggcg gccgcccact cggcggtgat cagcggctcg ttgtaggtcg acgtcacgac
     1861 ctgcgctccc gcctcgagcg cggcggccac gatcccctcc ggggtggccg ggcgcgggtg
     1921 cgcccagtca tccgcattcg ggtcgcggag cgtctggctg atctcccagt tctggcagta
     1981 cgggcagcgc aggtcgcagc cgagcatccc gaaggagagg gccgtcgagc cggggaggac
     2041 gtggaagaac ggcttcttct cgatcgggtc ggccgcgacg gaggccacgt atccccacgg
     2101 cgcgcggagc tcgccgtcgc ggacgaatcg gacccggcag accccctgcc ccccctcggc
     2161 gaggaggcag cggtgaccgc aggcgagaca gcgaagcttc ccgctctcct cccggacgag
     2221 cgtcgccttc gccgtcaggc cggcgaggtg ctcgcgaacc gagtcggctg gcgccatcgc
     2281 cctcccattc taggcgcgcc ggcagcgccg gaacggttcc tgcatccgag ccgttagaat
     2341 gcgccgcgtg acacgactct tccggatggc cgcgctcgcc gctggcgtcg cggcgacggc
     2401 ccttcctctc ttcgcccaga cgccgacccc caccccgacg cccacggcga cgccgacgcc
     2461 cgtccccccg acgtcgatcg aagcgtcggt cgccagcctc ggaatcccga acggcggccg
     2521 gtacgacgcg tcgaacatga tcctcgaggc cgacggcacg atctggaccg cctcggcgaa
     2581 cgagaacgtc ctcgcgcgga tctcggccga cgagaagaag gtccggaagt ggaccatgcc
     2641 gcgcgacgcg gcgccgagcc acctgctgaa ggagccggac gggaagctct ggatcgccca
     2701 gctcggcggc ttccaggtct ccctcttcga cccgcagacg tccgagctga cggagtgggc
     2761 cgacggcgcc cggcggccga cggcgttcgt ccggaagggc gacgggacgc tctggctccc
     2821 cgagacgaac ggcacgctcg cgaacttcga ccccgccacg gggacgttcg tctactggcg
     2881 ctcgaccgac gccgcgaacc cgatctcgag cctgacctat cccttcctgg acacggacgg
     2941 atcgctctgg tccgcggact tcctccgcgg ggcgatcctg cgcttcgctc cggacggctc
     3001 gaaggtgacg cgctgggcgc tcccggacgc gaccgcgcag ccttcgaaga tcatccgcgg
     3061 ccccgacggc cacctctggt tctccttctt cggggcggcg cagctcgccc ggttcgaccc
     3121 ggcgacgcgt gagctgaagc gttacgccgt cggcgtcttc tcccagccct tcgacatgaa
     3181 ggtctacaag gaccggatcg tctacaccga gcagctgatc ggtgaggtcg gagtcttcga
     3241 ccccctcgcc tccacgcccg cggcgaccga cacgctcgag gggaaggaga tcgtcctcac
     3301 gcggacgacg acgacggtgc cgccgacgaa gaaggtgctc gagacgatcg agctggacgt
     3361 cgcccccgcg gacccggccg cagtgaccgg ctcgggcctc cccggcctcg cccgctacac
     3421 cgcggtctcg ggagtcccct acgcgctggt cctcgacgag acccgcaagc gcttcctcgt
     3481 cggcgccacc gcggagatcg ccgcggtcct cccgcccgtc cccgcgaccg tggacgacca
     3541 cttcttcccc gccgcctcct cgatcggcgg ccgcggcggc gtgcgatggg ccacacaggt
     3601 cgtgacctgg aaccgcggga gcgcggatac ggccggggtc cgggtcgatg cgaccgtgaa
     3661 cgagcgcctc ctcccgacgg actggatcat cggcatctcc cccacgacga acctgacgat
     3721 cggcccggga aagatcgtct cccagtccga cccgatcggc gtggagatga aggggccgga
     3781 cacctccggt gggctccggc tcacctcggg cgcgacggcg acgcgcttcg gcgacgtcca
     3841 ctcgtggatc cgcgtctacc ggacgcgcga cgacggcggg acgtacggct tcgcgcggaa
     3901 ctacgtaaag ggggccgagg ccctcgcggc gggagagacc ggattcctct tcaccccgcc
     3961 cgacgccgga tctcgcgtca acgccggcct cctcgtcatc gaagcggccg aagggacggt
     4021 ctcgatcgtc gacgcggccg gcgctccgct ctcccccccg ttcgcgttcc gctggcccgc
     4081 cggctaccac gagcaggcct cgacgatctt ctccgccttc ggcatcccgc ccgccccgtc
     4141 tgcgcggatc gtctacaccg tgacgaaggg gcgtgtgctg ccgttcggca cctccatcga
     4201 cgccgtctct tccgaccccg tcgacctccc cttcttcggt ccgaggggaa ccgccgcgaa
     4261 ccagtgggtc ttcggcgtcg agaggggcgg cggtccgctc ggcccgacgt ctcgcaccga
     4321 cctccagctc ttcaacggcg ccgcgggcga ctcctccgtg acgctcgtcc tccgcgcgtc
     4381 ccgcctcgcc gcctcgcccg gggccgcgcc tccggcgcgc gccgtttccc tcacggtccc
     4441 caaggggaag gtcgtcgcgc tgaacgacgt cgtccgggag acgttcggca tcgacgtgtt
     4501 cgcggggtcc ctcgacgtcg tctccgatcc tcccgtccac gcgttcgccc gggtcacggc
     4561 tgcggacgcc tcgggcggcc gtcacggcta cgggatgccc gccctccgcg ccgactcgac
     4621 cgccgcggcc ggatcgcgcg ccgtcttcat ccaggcgtcc gacgccggct gggacgtcat
     4681 ggagtccgag ctccagctcg cgaatcccac cgactccgcc ggcgaggtga cgctccgggc
     4741 gttcgacacg gaaggcgccg cggcgggaga gccggtcacg ctcacgctcg gcccgaagga
     4801 atcggtccgg gtacccgcgg cgttcttcac gctctccggc ttcggcgcgc cgatcggccg
     4861 gatcgacgtc gcccccgtcg ccggctcgca gcccgtcttc gcgctgctcg tccgccggga
     4921 ccagaagacc ggggacgccg acgccgtcgt cccgtacgtc gtcccggcga cctgagcggg
     4981 cgcgccccga ttccggaaac cccggaggcc ccctcgtagc cgagggggcc tttttcgtgt
     5041 ccgcccgcct cgctagcggg gcgccggcgc gccgccggcc gccggaggcg cccacgtcgt
     5101 ctccggtgcg aggacccagt tccggtccgt caccgggtcg atggtccggg cgttccgaag
     5161 ccgctcgacg aggagctcgc gcacctcgtc gttgtaggcc cggacgaccc gggccccccg
     5221 gagcgccgcg tagcctcccg agccctgggc gcggtagctg ttcaccgcga gcgtgaagac
     5281 gtccgtgtcc ttcacctcgc ggccgcgata gcgcaggtcg cggactcgcc gaccggcggg
     5341 cgccgtcggg tcgatccggt agctcgcccc ctggacgacg tcgaagttgt agggccgcat
     5401 tccggcccgg ggcgtgagaa ggagccgccc gtccttccac tcggccgact cgtagaacgt
     5461 cgccgcgtgc tccaggatct cgcggagccg cgcgccgtcg atctcgacga cgacgagctg
     5521 gttctcgaag gggtagacgg agtagacgtc ccggaccgtg accggtcctt ccttccagcc
     5581 gtcgtggcgg aacgggagga gcgacgtcat cgagagctgt gcgccggtgg cggcgagctg
     5641 ggtctcgttg atcagatcga ggagcgcgga gtcctcgagg cgggcgcggt cggccggaaa
     5701 ggggccggtg gcggcggcga tcggctcgcc gaggtaggcg agcgcccgtt cgtggtgcgg
     5761 acgtgcgatc gtggcgacct cggggtccac cgcgaccgat tcgtccgaag ggaggagctc
     5821 tccgcgggcc tcgacgactc tcgccgtccc cttcccgcgc tcgacgacga ggtcgacgcg
     5881 ggcgaggacc tcgccccagc ggcccggctg cgtcacgggg acgccgtgga cgcgggtgag
     5941 ggggacgcgt cgatgcgagt gcccggtgag gatcaggtcg atccccggaa cggccttcgc
     6001 gaggcggacg acgtggttct cgtagtcggt cccgttggcc tctccggtgt cggggtcgac
     6061 ctcgaggccg ctgtggaaca ggacgacgac gacgtcgcac cgctccttct gccggaggag
     6121 ggggacgagg cgcgccgccg tctcgacggg gttctcccac tcgaggcccg gccggttcgc
     6181 ctccggctcc cagccgggga tgttcggcgt cgtgaggccg agaacgccga cccggacccg
     6241 gccgaacgtc ttcacgacgt actcggggaa cgcggggccg ccgccggcgg cgttccgggt
     6301 gttcgcggag agccacggga agctcgagtc cttctgcgcg cggcgcagaa cctcgaggcc
     6361 gaagttgaac tcgtggttgc cgacggccat cgcgtcgtag cgaagggcgt tcatcgccac
     6421 cgccatgaag tgcggccccc ccgtcggacg gcgggcttcg agccagccgg ccggcgcccc
     6481 ctggatcgtg tcgccgccgt cgaggaggag gacgtgcttc gagctcttcc ggatctcgcg
     6541 cacgcgcgtc gcgacgcgag cgagacccca gtcggccgct tcgccgcgcg cgtagttcca
     6601 cggaaggagg ttcgcgtgca ggtccgaggt ctgaaggacg gtgatggtga cgcgctccgc
     6661 ggcgagggcg gggagcgcga ggacgaaggc gaggaggagg aggaacgggc gtcggctgct
     6721 cacgcgcggc ggagtcttcc acccgctccg gcgggaatca aggcgcgccg gccgccgccc
     6781 ggcggtattc tccgcgcgca tgcccgcccg cccgaagccg aagcccgcgc ccgctcccga
     6841 ggtcctcgcc cgtctccggg ccgcgttccc gggcgcggac tgctcgctcg accacgccga
     6901 cgccttccag ctcctcgtct cgacgatcct ctccgcccag tgcaccgacg cgcgagtgaa
     6961 cgcggtgacg ccggccctct tccgcaggct cccgacgccg gccgcgatgg ccgcggccgg
     7021 ccccgagctc gaggagctga tccgttccac gggcttcttc aacgcgaagg cgcgctcgct
     7081 ccgcggcgcc tgccgcgcga tcgtcgagga gcacggcggg acggtgccgc ggacgatgga
     7141 ggcgctgaac gcgctccccg gcgtcgggcg gaagaccgcg aacgtcgtcc tcgggaacgc
     7201 gttcggctct cccgacggcg tcgtggtgga cacgcacgtc ggccgcctcg cgcggcggct
     7261 cggctggtcg ggcgccacgg acccggagaa ggtcgagagg gacctcaacg ccctcctgcc
     7321 gcacgcggag tgggtcttcg cgggccacgc gctcatcctt cacgggcgcc ggacctgcac
     7381 gtcgcgcgcg ccgcgctgct ccggctgcct cctcgaggag atctgcccga aggtcggcgt
     7441 ggcgcccgcg gcgaagccgg gcgcgggacg gcgcgcggcg ccggaggccc ggacgccgga
     7501 cgcggcccgc gcagggagga ggcgttgatg ctcccgggaa gcggctcgat cgaggtcatc
     7561 tgcggctcga tgttctcggg caagtccgag gagctgatcc ggcgcgtcac ccgggcgaag
     7621 atagcccggc gaagagtcca ggtcttcaag ccgaagctcg acgaccggtt ctcggaggtc
     7681 gaggtcgtct cgcacacggg aatgcggctc acggcccttt ccgtcgcgtc gtcggccgag
     7741 gtcctcgccc ggacggacga ccggacggag gtcgtcggga tcgacgaggc gcagttcttc
     7801 gacgcgggga tcgtggaggt cgcgagccgc ctcgccgacc tcggcaagcg cgtcgtcgtc
     7861 gcggggctcg accaggacta cctcgggcgt ccgttcgagc cgatgcccac gctcatggcg
     7921 ctcgccgagg aggtcgtgaa gacgcgggcg atctgcatcc tctgcggagg ccccgcgagc
     7981 cgcacccagc ggctcgtcgc ctccaaggaa cgggtcgtgg tcggggccgc cgggatgtac
     8041 gaggcccgct gccggcggtg cttcgacccg ggcgtcgtcg aggtcgacgg ggttctcccg
     8101 ttccccgaag agagctcctg acggctccga aaacgttcca gcggtgaggc ttatcgtctt
     8161 tcccagacgc gctgtgcata agcctgtgaa ttcggctgtc gaaaagtccg ggaagccgca
     8221 tcggaagcgg gccgcgcacg gaatgcactc ccatcggtgc acattcgcca agtgtctgca
     8281 ccacagggac ttgacaccac gcaagaaatg taaggtccgc gtgaagccgc ttccggagcg
     8341 gcccgaaacg atttacacag gagggggtcc gttctggcag cggttttgcc gagaagcgag
     8401 gaccgccggc ggccggtttc cggggcacgc cggcacccgg ggcccccgcg gagcccccgg
     8461 gtgccggcgc cgcccgctca aagcgtgacc gcgtcccggt gctcgccgaa gacggtcttc
     8521 agcgaggcga ccacctcgcc gagcgtgacg ttcccgtcga cggcctcgac gatcgggggc
     8581 atcaggtttc gcccctcctt cgccgcggcg acgaggtcgg cgcggagccg ttcgaccttc
     8641 gcgtcgtcgc gccgggcccg gaaggcggcg agccgctcga cctgctccgt ctcgaccttg
     8701 gggtcgatcg tcatcgtggg gatcgggttc tcctcctcga ggacgaactc gttcacgccg
     8761 acgacgacct ggctcttcga ctcgatcgcc ttctgcgcct ggtaggccgc ctcctggatc
     8821 tcgcgctgga agtagcccgc ctcgatcgcg gcgagcgccc ctcccatctc gtcgatcctc
     8881 ccgatcagcg ccgacgcctt ctcgacgatc tccgcggtga gcgcctcgat cgcccacgat
     8941 cctcccagcg ggtcggcgac gcgggcgacg ccggtctcgt aggcgatgac ctgctgcgtc
     9001 cggagcgcga ggcgggccgc ctccgtcgtc ggcagcgcga gcgcctcgtc gcgcgagttc
     9061 gtatggagcg actgcgttcc gccgaggacc gcggcgagcg cctggatcgc gacgcggacg
     9121 acgttcacgt cgggctgctg cgccgtgagc gtcgagccgg ccgtctgcgt gtggaaccgg
     9181 agcgcctgcg cgcgcgggtc gtcgatgccg aaccggtcct tcagcaggcg cgcccagagc
     9241 gtccgagccg cccggaactt cgcgatctcc tcgaggaagt cgttgtggac gttgaagaag
     9301 aaggtgagcc gcggggcgat cgccttcgcg tcgaggcccg cccgcacggc ggccgagagg
     9361 tactcgacgc cgtcggcgag cgtgaaggcg acctcctgca ccgccgtcga gcccgcctcg
     9421 cggatgtggt agcccgagat ggagatggtg ttccagcggg ggacgttctc cgcgcagaag
     9481 ccgaagatgt cggtgatgag gcgcatcgac ggcttcggcg ggaacgcgta ggtcccgcgg
     9541 gccgcgtact ccttcaggat gtcgttctgg atcgtccccg acagcgcctt ccactcggtc
     9601 ccgcgccgcc gcgcgacggc gaggaggaac ccgaggagga cggaggccgt ggcgttgatc
     9661 gtcatcgaga tcgaaacgtc ccccagcggg atgccgtcga ggagcgtctc catgtcctcg
     9721 atcgagtcga tcgcgacgcc gacccggccg acctcgccgc gcgcgagcgc ggcgtcggag
     9781 tcgaagccga tctgggtcgg gaggtcgaag gcgacggaaa ggccggtctg cccctgggag
     9841 aggaggtagc ggtagcgcgc gttggactcg cgggcggtcg agaagccggc atactggcgc
     9901 atcgtccaga gcttcttccg gtagccgccg gccatcgcgc cgcgggtgta cgggaacgca
     9961 ccgggcacgc cggggtccca gccctgcggg acgtcccccg gggcatagga gggacggacc
    10021 gggatccctg aggtcgtggc ggtgtcgggc ttgttggtgg acatgggacc gatgctaatg
    10081 gaaaccgtgc gcgccgacac cgtgcgagac cgcatctcga atcgacctct ccgcgttccc
    10141 gcgcttcgcc cggaccggcc gcccggccga acggccgatg ctcccggcgg ccggctccgc
    10201 cggagcgctc ttcgtgtcac acttcgccca gccttgtcga agtcgctcag cggaacgctc
    10261 gataccttcg ccctcgccga cctcctgcag tggatcgaga tcaaccggct ctccggccgg
    10321 ctgaccgttt cgcgcgggga ggaccgaagg atgatcgacg tgaagaacgg cgcgatcgtc
    10381 ttcgtctcgt cggtccggcc ggaggaacgg ctcgggacgt tcctggtcgc gcgcggcatc
    10441 ctgccggagc cggtcgtcta cgagctcctc gccgacagct tcctgacggg ccggagcctg
    10501 acgaggctcg tcttcgagac gggcctcctc tctcgcgacg cgctggccgg ggccgtcgag
    10561 gagctcgcgc tgaaggtcct cttcgacctc tttcactggc agggcgcgcg gttcgtctac
    10621 gacccgacgg tcctcaccga ggacctcctc cgtatccagc tctcccttcg cggccagctc
    10681 ctcgcgctcg agggggcgag ggccgtggac gacacggcgc ggcgtcgcac cgcggagtcg
    10741 gtggaagccg aggagcccgc ctcgaacgcc gaggagctct cgccgcggaa cgtcgcacgc
    10801 tcgttcttcg ccgtcgtcga gggcctcgac atggagagcc ctcccccgtc cgtctggcag
    10861 gaacgcttcg agctcttcgg gaggttctcg gaaaaggtcg cggcctcgct gcgaagcccg
    10921 ttccgcccgt tcccgctctt cgaggacacc gccgagcgct gccgcgccgc cctcgaatcc
    10981 ggcgcagaga ccgaggagac ctttcgcctc gccgcgacgg acccgttcct cacggtcgac
    11041 cttctcttcc tcgggaacgc cctccggacc cccgccacgg ggctcctgtc gacggtcgac
    11101 ggcgccctcg ccgcgatcgg aacgggggct ctccgcaggt tcctcgagct gctcgtctcc
    11161 ccgggcgctc cggtggtctc gggccgggat cgcctcgagc gggcgctccg gagcgccgcg
    11221 gtgtccaccg ccgtggccgc gtcccacgtc gccccgtcgc tcggagagga ccccgggcga
    11281 gcctgggtcc tcgcgctcct cgagccggtg ggcggctacg agctgctcaa gctcctcctc
    11341 acggaggact tcgacccggg tccgttccgt gccgccaccc tcggctggta tcgcgcggcg
    11401 agcggccgcg cgctggcacg gaagctgaat ctgcccgatg cgttcgccga cgtcctcggc
    11461 tcggacggcc aggtctcgag ccggagctcc gtcgccgagc agctggtctt tttcgccaag
    11521 cagatggtcg cgggggagca ggtcggccgc gagtggacga gcgacgaccc ggagctcgcc
    11581 gaccggtacg ccgccctcgc cgtcgacgac gcgctggcgg accggatcgg ccgggacgcc
    11641 tcggccctgc gcgagctcct cggtctctga tccgccggcc cgtctcttgc cgtagtccca
    11701 ggtagcccgg tcgttccggc acgttccgaa cggaggtatc ccatggtcgc ccgaaatgcc
    11761 gcatccgcga tcctcgtcct ctccctcgcg ttcgccccgg gctccgcctc tctcgccgcg
    11821 gagccgccgg agacggccct tccggccgga gtgcgcgcgg ccgtggaggt ccgcgccctg
    11881 gacctcgacg tcgtcgcgac gaaggacggc cggccggtca cggacctcac ccgggacgag
    11941 ttcgtcgtcc gcgtcgacaa gaaggtcgtc ccgatcgact acttcgcccg cgtcgaggcc
    12001 ggcacggtcc acgggcccga cctcgcgacc gcctcgccgg acctcgttct cgacaccctc
    12061 cgcaacgacg ccggcgagac ctacgtcccg aggcagttcc tcgtctactt cgacgacgag
    12121 cggctcctcc cgcaggaccg caagccggtc ctcgaggggc tccgggactt catgacccgg
    12181 ctctcgccct ccgaccgggc ctcgatcgtc tcctacaacg gggcgacgcg ggtcctcgtc
    12241 cccttcacga gcagcaagga ggacctcctc gacggcctct cgaagctcga gacgctcgcg
    12301 ccccgcgggc tcgccctcga gtcggaatac cagaggacgg tgagggagat ccggatctcg
    12361 cctcgctcac gggcttccct gatacgcagc tggtcgacgg aggccgcgca ccgcgagcgg
    12421 tgggcgctcc gcgagctcgc ccgaagcgtc tcggccctcg ccgcgcggtc gggcaagagg
    12481 acgctcctcc tcgtcacgaa cggctacgaa tggcaccccg gccgggccct cgccctggcg
    12541 tacggcccga gcctcgtctc gcagttcgcc gaggacgtcg gcgaggagat cgagaaggtc
    12601 ctcgccgagg ccaacgccgc cggaatcacg atccaggtct tcgacgcgaa ggggctcgga
    12661 gccggcgtcg acgcgtccga gcggtcgaac cccgcgatca gcccgttcta ccgcgcgcag
    12721 ggctaccgcg actcgatggt gactctggcg accggcacgg gcgggtctct cgtcgagaac
    12781 cggaacgagt tccggtccga cctcgatcgc gtctaccggg aggcgagcgg ctactactcg
    12841 gtcggcgtca cgctctccgg cctcgccggt accggcgagc gaaaggtcga ggtgcggacg
    12901 acccggcccg gagtcgtcgt gcggacacgc aacggcttcc tcccgcgcag cgccgacgac
    12961 gccgccgtcg accgggtcga gatggccctc ctgacgcccg gcgccccggg ggacttcccg
    13021 gcgacgcttc ggatcggctc cctctcgccg gcgggcgggc ttgcccgtcg gacgggtccg
    13081 tacgaggtgc agttctccct cgaggagctg acgttccgcg aagagggaga ccggaagacc
    13141 gccgtcgtcg acgtcacgct ggcggcgatc caggacaccg gggcccgctc cgacgtgaag
    13201 ccggagagga agatgatctc catcccggcc tcggagtggg agcgcgtgcg cgccgagccg
    13261 ttcgtctacc gcggccaggt caagagcgga aaggggaaca tccgtttcgt ggccggcgtg
    13321 cgcgacgtgg cgaccgggcg gatggccctc gcctcggcag acctccgagt cgagtaggcg
    13381 ccgcggcgcc tcccggcgtc cccccggacg aagaacggcc ccggctcgcg ccggggccgt
    13441 tcttcgttcg cccggtcggg cgggggctac ttcttcgggg cgggggcctt cggcgccgcc
    13501 ggcttggcgg ccggggcctt cgccggcgcg ggcggcgccg cgagcgagaa cggcgtcttc
    13561 gcctcgacgc tcttgccgga gatcacgtcg gtgatcttca gcgtcatgac gtactcgccg
    13621 gggcggaagt aatcgccgag gttcacgtcg acgatcgagc cggccaggtc gatgacgaga
    13681 gcggtcgccg agccggcctg ctccggcacg accatcggct cgtccggcgg ctggggcacg
    13741 tcgatcgtgg agccgttctt cggcttgatc gagatcgacc gcttcaggtg gagcctcttc
    13801 gtcgcggggt cgacggcggg gttgtagacc cgggccacgt aggcgaggcc gtcggtcttc
    13861 tcgagggtgt cgccgcccct cgcgacgaac ttccggaccg agaagacgaa cggctcctcc
    13921 ggcttcgccc cgtcggccgc gatgtcggtg cacgcgagga ggagcggcga catcgcgagc
    13981 tcggacggca gcgggatcac cgtgacgggc cggtaggccg agaccttctc ggcgcccgag
    14041 gcgtcgagga gaagcgcggc gacctggtac tcgccggcgt cgaccgggag ggcgacgtcg
    14101 acgaaccagt cctcccgcac cttctcgagg acggcgggct cctcgcgacg ggcggcgtcc
    14161 ttcccgtcct tgccgcggac gaggagggcg atcttcatcc cctccggggc cgctccggcc
    14221 gcgcccgcgg gcaccgccga ggccgggacg tagagctgga ccatcaggcg ggtcgccttg
    14281 tccccgtagg cgagcgggaa gagggtcaga gagccctggg gctccttgcc gaggagcgcc
    14341 tcgagagagg cgcggaccgg cgctgacagg accggcccct tggcggcctc ggcggcggcc
    14401 tcggccgcgg ccttcatctc cgcctcgtac tcctcgcgcg tcttgtagac cggaggctcc
    14461 gtcatcttcg ggttgacgat ggcggccgcg atcgccttct tccagagctt cttgatgtcg
    14521 gagctcttgt cggcagactc ccgctcggcc cgctcgtcga ccgtgaagtt gacgcggaac
    14581 gacttcatcc cggcccactc cgggagctgc tccttgtcgt actggaactg gatcacgacg
    14641 acgttgccgc cggtctggag gaacgaccct tcctcgccct ccccgccggc cgccccggct
    14701 ccggccgact cgagcttcga ggcgatcgcc ttcggcggcc cgaggaggat cagcaccttg
    14761 ccgcgctcgg tcagggcgcc gcgcttcttg ccgagcgtga agcgcttgtc ggcctcggcg
    14821 acgagggcgt cgaacctcgt ccggaagacg ttctgggcgg tcttctggta gtcggggtgg
    14881 cgcttgcccc agaagaggtt gaagaacttc tgggcctctt cgtcgttcgt gacgccggtc
    14941 caggccttct tctcgtcgtc cgtggcgtag tagacgaact cgggggagct ctcccagtcc
    15001 ttgaacttat ccagctgggc ggccgcgggg agcgcgagcg ccacgagcgg gacgagggtc
    15061 gcgagaagcc ggagctttct catccgtgtg gtcctttcag ggggggttcg ccgggaccgg
    15121 cgcatccttg ggactatatc gcaccgcgcg cgggagcatc gcggcggaac gcgagagagg
    15181 tcaggagctc ccgaggagcg cctggacctg ggggtcgtcc ggccggatct tcagcgcctt
    15241 ccgaagcgcg cgctgcgccg cggcctcgtt gccgagcgcg cgctcgacgc gcgcgagccc
    15301 caggagcgcc tcgaagaggt tcttcccttc gcggtacgcg cgtcgatacg cggtccgcgc
    15361 ctccttgaag ttgccgcccg cgagctccag gtcgccctgg acgacgaaca ggtaggccga
    15421 ggcgtcccgg gtgttcgcgg ccgcgagcgc ggcccgcgcc tcggtggacc tcccctgctc
    15481 gatgtagagc gctgccaggt tgtgcaggag ggccggtcgg tgggggtcga ggtcgagccc
    15541 ctcccggaac gcctggtagg ccccctcgat gtcgccctgc ctccacctca cgatcccgag
    15601 gttcgaatga aggtccggga gcgagcgctg cagcttcgtc gccctctcga ggagcgttcg
    15661 cgcccgggag aagtcccccg cccggagagc ctcgaccccc cggttgctcg cggagatcgc
    15721 ggcgacggcg aggtcgtcga tcagcttcag gtcgacccgg gactcgtcgc gggtcatgta
    15781 gaagtcgtag acggtcaggt gcctcagctt cggcaggacg gcgaccatgt ggttgtagac
    15841 catcacgagg tcgccctcct tctcgctccg ggcccgccgg aagacgagcg ccgcctggag
    15901 ggggatcccg agggcccgtc cgaacgcgat gaagaggttc gtgaacgaaa cgcagttccc
    15961 gcggcggaac gcgaacgcct ccgccgcggt gaaggtcgcc accgtgtcgt actcgaacgc
    16021 gtagctcttc cggtcgagga tggccgcctg gagccggcga agcctctcgc cgtcgtcgcc
    16081 cgccggcccg gcgaggtgct ccgccgcggc ccggatctgc ggcgtcacca tcatcggcat
    16141 cgggacctcg ccggggtcga cgccgcgggc ggcgacgacc tcgcgccatc gctccggcgt
    16201 gaggcggagg accggcgtct cctcggtcgt ccggcagcct cccgcaaaga ccccgaacgc
    16261 gaggacgagg acggcgccgc tccgccaggg ccgaacgaga ggtctgatca cggcacctct
    16321 gattacgccc gcaaccgcgc tcgggttcaa gccccgtttc gaccgaccgc ccgaagcccc
    16381 cctccccggg gcggcccggt gccgacgtcc gggtccccgg ctccgccggc cccggggacc
    16441 cggcgtgcaa gaatgaaggg ttgtgcgcgg gaggaccccg cgccgcccga acgccgtctc
    16501 gcgaggtcgc cgtgatccca ggcctgaaaa atcgagtcga agccgccgtc aaggacgcct
    16561 gccgccgcct gtgggacgtc gagccgccgc ggctcgtcct cgagacgccg ccgaaggtcg
    16621 agctgggtga cctcgccctc ccgatcgcgt tcgagctcgc gaaggtgctt cgccggccac
    16681 cccggaagat cgccgaggag ctcgccccgg cgctcgagct gcccccgatc gtcgcccgcc
    16741 ggagcgtcga ggggagcggc tacgtgaacc tcttcctcga ccggccgcgg gccctcgccg
    16801 cgatgctcac gcccccgccc gaggccgagg ccgtccccgg caagatcatc gtcgagcaca
    16861 cgaacatcaa tccgaacaag gccgcccaca tcggccacct ccggaacgcc gtcctcggcg
    16921 acgtcctcgt ccacctcttc cgccgcctcg ggaagagggt cgaggtgcag aactacctcg
    16981 acgacaccgg cgtccaggtg gccgacgtcg tcgtcggcct cgagcacccg gacctcctcg
    17041 ccgagccggg ccgggccgag gagctcgggg ccgagatccg cgagatcctc tccgcgttcc
    17101 ccgacgacgc ccccggcacg gagccgcgcc ggctcggcga cgtgacctgg gacctctacg
    17161 ccctggtcac ccggagctac gccgccgacc cgtcgctcga agagcggcgc aaggcgactc
    17221 tccacgcgat cgaggaggcc gccctcgggc acgagatgac cgacgagggc cgtgcgaccg
    17281 cggctctcgc ctcccgcctc gccgaggcgg tcgtgcgctg ccacctgaag acgatggagc
    17341 ggatcggggt ctcctacgac ctccttccgc gcgagagcga catcctcggc cgccgcttct
    17401 ggc
//
LOCUS       DAOTEZ010000029        17383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2212_length_17383_cov_8.094933, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000029 DAOTEZ010000000
VERSION     DAOTEZ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17383
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2212_length_17383_cov_8.094933"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="P5164_02595"
     CDS             complement(<1..372)
                     /locus_tag="P5164_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRY42812.1"
                     /translation="MTIRRQILVAAAATALLLGGPAAAQEVKGEEHVLPNGMKLLLVH
                     RPDEPSVSAGWVAKVGSANERVGMTGISHLFEHMMFKGTKTLGSKDPKRDLEIIEEQE
                     TLRGEMRAEESKMRAAWRRGEV"
     gene            complement(511..1218)
                     /locus_tag="P5164_02600"
     CDS             complement(511..1218)
                     /locus_tag="P5164_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRY42813.1"
                     /translation="MRREYHAWVSHHLGRTMEFLWLGERGYPVLLFPTSMGRFWQNED
                     MGITGALAEKVDAGYIQLVCVDAIDTESWYNRNAPPAYRVRRHDDYDRYLRHELAPYV
                     QHRADRPDLGVYGASFGAYHAASFAGRYPGLVTKAVLLSGLYHVGRFLDGYWDDLCYY
                     HCPEAFVANADEKTVRALRQVEWIVATGEDDTLVGENRHFAGLLEGKGIGVRAEFWPG
                     VFGHDWPFWREAVRRFL"
     gene            1309..2265
                     /locus_tag="P5164_02605"
     CDS             1309..2265
                     /locus_tag="P5164_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42814.1"
                     /translation="MSAKTIGVLFGMEDTFPWALCHEINELARRRGLAVKGEPVRVGH
                     VSQEQAFGYDVILDRISHEVPFYRTFLKCAAARGVQVVNNPFWWSADDKFFDNVVARA
                     VGVAVPRTVLLPHKEHPPNTTDASFRNMVLVDWDEVFRYLGFPIFLKPAYGGGWKDVW
                     KVHSREELFSAYDQTRDLTMMAQEAIEFEDYYRCWVAGRRKVRIMPYAPKEPHEARYS
                     AVAGKVVPDEMALRVTKDALALCDALGYDMNTVEFAVRDGIPYAIDFMNCAPDADLHS
                     VGEESFRWIVTEMAETLVERALHPIPWEPTGSWPKALGLLPR"
     gene            complement(2293..3396)
                     /locus_tag="P5164_02610"
     CDS             complement(2293..3396)
                     /locus_tag="P5164_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42815.1"
                     /translation="MKTLLPGLGTAALGLAVLFTFALALTTKMFTLRPPSGPDAMGLV
                     VVLFLPVAAWVLVLVAALAGVGHGGFDWVSRSPGVPTLAVLATVVGLGILSTAAAIFS
                     LEVRYSSRTVLGFAGGLVLPLAVVAFLGALLWSEPAAVSAARWPRPAGVALAGLAALS
                     FAGGLVFLAMTSAEKARVVEEARQANEDWEARNRREMDALREKQAAELAALPDDAPIE
                     VFLSHLFIDKSEEHHQQALERIRALPDLTARLAARLEQPEPLEREHVLNFIAMAGTPE
                     PDWEPLVRTALVRLAADYRAEAKDLSIGRITHVKGLSWGALLAAERFGPRRFEEEARE
                     LRAAVALWPNEGPRKDALAVIDLYLEGSPLPRE"
     gene            complement(3439..4164)
                     /locus_tag="P5164_02615"
     CDS             complement(3439..4164)
                     /locus_tag="P5164_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRY42816.1"
                     /translation="MKTRLTALLVDDERLARAELRRMLAAHPEIEVAGEAGDVAEAEA
                     AIARLSPDVVFLDIRMPGASGFDLVEKVPAGTRVVFVTAYSEYALRAFEANALDYLMK
                     PVPPERLAAAVRKLLDGAPAASAPGRPLHLDDPLIVPSGTHYRVVRVRQIVCLRAARD
                     SSEAVTADGRTTLVGRSLKEWEELLPPRHFLRVHRSTIVNVEHVERIEPWFKAGFRVR
                     LRGLPEPVEVSRRHAALLRERLV"
     gene            complement(4161..5198)
                     /locus_tag="P5164_02620"
     CDS             complement(4161..5198)
                     /locus_tag="P5164_02620"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRY42817.1"
                     /translation="MKLPPRPFWIAQLAGWGSYFLILWLSGLAYAAEPSFRALLEFLP
                     AKAVLASLGFATSLGLFAVYRHLLPERPRTGRLVLVAVAASLLFGVVWSAAASRLLWP
                     EKPVWPGAFKSGLNSGITLLAWSALYLAFTYRARLDAETERALRADALASQARLEMLR
                     YQVNPHFLFNALNSIRALIEEDRAKARQMVTELSEFFRYSLLHARSGEIPLEAELEAI
                     RSYLAIQEIRFEERLEVTWEVEESAKAARLPGFLVHPLVENAVKYGMETSPMPLRVGI
                     SARLAAGRLVVEVTNTGTLGSNGTCEGTGTGLANVRERLAHHFPGRHAFTIAQEGERV
                     VARLEVPAEGA"
     gene            complement(5220..5672)
                     /locus_tag="P5164_02625"
     CDS             complement(5220..5672)
                     /locus_tag="P5164_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRY42818.1"
                     /translation="MKRTLALLAAIVLLALLLVPLIATSLPPGEADPAVRAPVEAYLR
                     GHATGDGEEWRKAFHPIAMVTGVRDGKLVSMSAPDFVARAAGKPAPDEAQRKRRIVSI
                     DVAGDAALAKVELDYPKVFFVDYLSLLKIDGEWKIAQKTYTGTSKTAP"
     gene            5890..7593
                     /locus_tag="P5164_02630"
     CDS             5890..7593
                     /locus_tag="P5164_02630"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006005033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRY42819.1"
                     /translation="MRGFGVLVAVLALGALAAAPEGERADLVLRNGKLLTMDPERPVA
                     SALAIAGDRIVAVGSDAEIDRLRGEGTRVIDLAGKAAMPGFIDGHAHFTGIGDARIAL
                     DLRGAASWDEVVARVAAAAKKARPGEWILGRGWHQKEWASAPSPNVEGFPLHASLSAA
                     TPENPVLLSHASGHAAFANAVALERGGVGRDTKAPDGGEILRDASGAPTGLLRETAED
                     LVSAALQKDLARRTPAEVTADLDRRIDLAVEECLRKGITSIHDAGSSLDVVARYRQRA
                     EAGTLGVRVYAMLSSSNEELEREAARVRVIGAGRNHLTVRAVKRLADGALGSRGAWLL
                     APYADAPGTGLCTYPMMDLERTAEIAAANGFQLGTHAIGDRANREVLDVYARVLARHP
                     RKDLRFRIEHAQHLDPADVPRFGKLGVLAMMQGIHCPSDAPFVPARLGAARARDGAYP
                     WRRLLDAGARIGNGTDAPVEDVDPIASLHASVTRQPKKGPAFFPEQRMTRMEALRSYT
                     VANAYAAFEEGIKGTLAPGMLADVVVLSKDLSTVPDAEIPSTRILATVVGGQVVFEAS
                     R"
     gene            7590..8339
                     /locus_tag="P5164_02635"
     CDS             7590..8339
                     /locus_tag="P5164_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42820.1"
                     /translation="MRRLDAAWAAARARARARAFPPGSVTFHLGAWSRGRLAEDEAEA
                     ARALLEHHGFSRLLVLHGSALEVDGAVVLVVGPRGIGKSTACRELVRHGRGKLLEDGL
                     LVVGEHGGAWRLVETGTLPVLRRTARLKAGPRRLLPRVEGMPHPGERWAEGLRRRLHR
                     ALDEQAFRAAVLLAGGRPAGSVGCLRTVGRVVVAEEPFTPSGSFEIDGRGMTEVPDVA
                     SRVPASCGVRRLAATGPRAEVVARLVAAMRG"
     gene            8440..8925
                     /locus_tag="P5164_02640"
     CDS             8440..8925
                     /locus_tag="P5164_02640"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRY42821.1"
                     /translation="MARTSRDPETSLLLDLLAEAYERRSWHGTNLRGSFRGMTAAEAA
                     WRPAEGRPNAWELALHAAYWKYAVRRKLTGEKRGSFPLEGSNWFRRPEGEPAEEAWKA
                     DLSLLAAEHRALVAAVAERLPGLTGPARAKALHLVRGVAAHDLYHCGQVQALKKLRPK
                     G"
     gene            8922..9647
                     /locus_tag="P5164_02645"
     CDS             8922..9647
                     /locus_tag="P5164_02645"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRY42822.1"
                     /translation="MTGGFFPPTRASVVDALRSGDAAERERALETLVAAYWRPVYRHV
                     RRKWGKGHEEAADLTQDFFAELLSRHLLARFDPGKARLRTYLRLCVDGLAANAAKAAS
                     RQKRGGGAAPLSLDFEGAREELERAAPAADETPERAFEREWVRSVLALALERLPSALE
                     GRGKGIAFALFELCELGAEAEVPSYAELAARFGVSVSDVTNGLSLARRELRRIVLDLL
                     RELTGSEEELRLEARAVLGIDAP"
     gene            9644..10630
                     /locus_tag="P5164_02650"
     CDS             9644..10630
                     /locus_tag="P5164_02650"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRY42823.1"
                     /translation="MTALPDAALSRLVRALRTPDLSETRYLLGREVGRGGMGSVWEAD
                     DPELGRSVALKVLDLPDSAGDLSARLRREARVLARLEHPGVVPVHDVGTLPDGRPWYA
                     MKLVRGERLDAAAARLSTLPERLRLFLRVCEPVAFAHARGVVHRDLKPSNVMVGPFGE
                     VLVLDWGIARLLEEEASEGRPGPPAAPPTEPREPRAAAAPGTAAGTVLGTPGYMAPEQ
                     ARGDGDVDGRADVFALGVLLRELAGPEALRARRLAAIVAKATAERPAERYGGAPALAA
                     DVVRYLDGEPVTAYRERPWERAARFAVRHRVALLLVLAYLAARAAILVLYRR"
     gene            10678..11043
                     /locus_tag="P5164_02655"
     CDS             10678..11043
                     /locus_tag="P5164_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42824.1"
                     /translation="MNKLLLGLLLGGGLGVLDGLTAWFTPEVRKDILGIVMGSTFKGL
                     VAGLLIGFFSKKVASVPATVVFGVIVSGFFAYLVAAQMGKYYFEVMLPGALVGLVTGY
                     VTARYGKGGPAGNPERRLA"
     gene            11139..11612
                     /locus_tag="P5164_02660"
     CDS             11139..11612
                     /locus_tag="P5164_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRY42825.1"
                     /translation="MAGKVNPIPEGYGTVTPYLCVKGAAAAIELWKKALGAEELFRMP
                     GPGDRVMHAEVKIGTSIVMLSDEFPDFGQLGPTSRGGSTCTMMMYVPDCDAAYKRAVE
                     AGCTPVSEPKDEFWGDRFAKVTDPFGHQWAFATHVEDVSPEEMERRAAAMFEKGC"
     gene            11722..12303
                     /locus_tag="P5164_02665"
     CDS             11722..12303
                     /locus_tag="P5164_02665"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRY42826.1"
                     /translation="MKKPLGLAALVLLALVLAAPPAAADALSLELGLHGGLVNSKDGE
                     GGSFLGGAQARVNLFWIVAAEARASYYTDSYDLGQIGSVDVENIPFQVSGMIYPIKLP
                     KVGVYLLGGGTYSNLKVKGTSVVQGEVTEKGWSTHVGAGIDIKVGEKTTLNGDVRAVF
                     LDANSVDELFDSVKSSYKGDFWTVTLGLNYKLF"
     gene            12398..13378
                     /locus_tag="P5164_02670"
     CDS             12398..13378
                     /locus_tag="P5164_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HRY42827.1"
                     /translation="MPAASLLALLDDIATVLDDVAILTKVAARKATAVADDVAVLSKV
                     AAQKTAGVLGDDLALNAEQVTGVRAERELPVVWGVFKGSLVNKAILVPAALAISAVAP
                     WAVTPLLMVGGLYLCFEGFEKVAHKLLHRKGEDEVRHAELAEALLKKNVDLAALEKEK
                     IKGAVRTDFVLSAEIITIALGIVANVPFGTRVLVLTAVGLAMTVGVYGLVAGIVKLDD
                     LGLYLRQKEGASRLARLRRSLGGGILSAAPWLMRLLAVVGTAAMFLVGGGILVHGLPP
                     LHHLAEGLVARGGLVGGIGPGLLGGLVGVVAGGLAVGVLTAGGRLFRRKR"
     gene            complement(13428..14411)
                     /locus_tag="P5164_02675"
     CDS             complement(13428..14411)
                     /locus_tag="P5164_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HRY42828.1"
                     /translation="MTSATPIHDFILANYRNFNSRATKDAMLAYWKHVEAGGKMFWAV
                     AGAMSSAQLGITMAPAIRAGLIHGLSVTGANLEESLFRLVAHRSYKDFPDYRYLTKQD
                     DTRILEDRMRRVTDTSIPEDEAFRAVEKYVVPMWKRATERGERRFWHEYFYELVTTIP
                     ADLYEGNPDECWLLAAARAGLPIVVPGHEDSTFGNIFASYVKTGECSPSIVKSGIEYM
                     AAFYDQYRTLSEGAGVGFFQIGGGIAGDFPICVVPSIKYDLQEPVKPWAYFCQISDST
                     TSYGSYSGATPNEKITWDKLTEETPMFVIESDATIVAPLITTALLELKAAR"
     gene            complement(14542..15639)
                     /locus_tag="P5164_02680"
     CDS             complement(14542..15639)
                     /locus_tag="P5164_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42829.1"
                     /translation="MHVLPSLLLALPILASGAPTPTEPAGKSLSAEVSALTRPLVEPA
                     YRKANGRGFSTFSCDVPKPVPPGGSFDCDAVDEKGEKTRYTLAVDGDGQATIVVVSFP
                     ASRLEPEERALVEPPCRAFLEAYRRAAWKEVHAALHPALRSELPLAALEAQLGPARDS
                     MGALGSVSVRSVSVRSTPDPEVRHSELVWDLDSEKGPGVARFRIESDGEVPRVSAFRI
                     YPAAGSALQASVLEAMLRGKASELLGEPVARLAGPLEKLEKVGDAVLGTAHLASGGEV
                     PVRIEQSGRRDDFERNDFTCQILDAPWLIRKSFASRSLELSSVDCPSRVVPDDGSSVC
                     DVRLKSGERYAVTIQRRGGEHRMTAEKAPDR"
     gene            complement(15763..16083)
                     /locus_tag="P5164_02685"
     CDS             complement(15763..16083)
                     /locus_tag="P5164_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42830.1"
                     /translation="MAPRLAYGPRDVGRRIAYGYAAAAVVLLSVLALRADAFEGWMWG
                     SLMILMFLVVSLRSALVWVFRKRPSRLSWWFHPTPGVENEFTVAWLVSAVLFGAFSVL
                     PVLL"
     gene            complement(16237..>17383)
                     /locus_tag="P5164_02690"
     CDS             complement(16237..>17383)
                     /locus_tag="P5164_02690"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY42831.1"
                     /translation="VLRKEEHGVAVGLRTAVYRAAMTLSGGLAIWAAASLGWGAVNLL
                     LGLVYLPLLLVTWRSPEPEEEAAAPKTLRDAVWLPFLECLSRHRAVEILVFVVLYKLA
                     DALSQSLLRPFLIDMGYGGFDRGFVLGTAGVALTIAGTFLGGVACTRIGLGHALWIFG
                     FFQIFSNIGYVLITYQPGSRPVMYGAMAFEMVTSGLGMGAFGVLLLRMTEKRFSATQY
                     ALFSSLFGLPRILGGPITGLIVDAAGWRTFFWFTIAAGVPGLLLLQRFAPLGTREPVF
                     TVETAEPRRRLTSRQIARRSLLGAAVTLVASGLWMIGLVAVKAYRKDPAAGFDLAAAS
                     AAFFRPAAVGGWIQLAALLVVAVLGGLATAALLTARHGERETERRTT"
BASE COUNT         2504 a         6082 c         6449 g         2348 t
ORIGIN      
        1 gacctcgccg cggcgccagg ccgcgcgcat cttcgactcc tcggcccgca tctcgccgcg
       61 gagcgtctcc tgctcctcga tgatctcgag gtcccgcttc gggtccttcg agccgagagt
      121 cttcgtcccc ttgaacatca tgtgctcgaa gaggtggctg atgccggtca tgccgacgcg
      181 ctcgttcgcg ctgccgacct tcgcgaccca gccggccgac accgacggct cgtcggggcg
      241 gtggacgagg aggagcttca tcccgttcgg gaggacgtgc tcctctccct tgacttcctg
      301 ggccgcggcg ggtcccccga ggaggagggc cgtcgcggcg gcggcgacga ggatctgtct
      361 gcggatggtc atggacacct caccttgtcg gacgtgtacg gcgggagacc ggatcgggtt
      421 ttaggtccgg ccccgatgtc gttttctgag aacggtttgc aggaatgtgt gccgccgcgt
      481 gggacggacc cgaagggcgg aggaggggct tcagaggaat cgccggaccg cctctcgcca
      541 gaacggccag tcgtggccga agactccggg ccagaactcg gcgcggacgc cgatcccctt
      601 gccctcgagg aggccggcga agtggcggtt ctcgccgacg agcgtgtcgt cctcgcccgt
      661 ggcgacgatc cactccacct ggcggagcgc ccggacggtc ttctcgtccg cgttcgcgac
      721 gaacgcctcg gggcagtggt agtagcagag gtcgtcccag tagccgtcga ggaaccggcc
      781 gacgtggtag agccccgaga ggaggaccgc cttcgtgacg aggcccggat agcgcccggc
      841 gaagctcgcg gcgtgatacg cgccgaacga ggcgccgtag accccgaggt cgggacggtc
      901 ggcgcggtgc tggacgtagg gggcgagctc gtgccggagg tagcggtcgt agtcgtcgtg
      961 gcgcctcacc cggtatgcgg gcggagcgtt ccggttgtac cagctctcgg tgtcgatggc
     1021 gtcgacgcag acgagctgga tgtagccggc gtcgaccttt tcggcgaggg cgccggtgat
     1081 ccccatgtcc tcgttctgcc agaagcgccc catcgaagtg ggaaagagga ggaccgggta
     1141 gccccgttcg cccagccaga ggaactccat cgtccggccg aggtggtggc tcacccaggc
     1201 gtggtactcg cgcctcatgt gcggagtcta tcgggagcgg agctttgcgg cgcctcgggc
     1261 gtccgatacg atcccggcgc ggaaggcgcc gcatccgagg aggtccgcat gagcgcgaag
     1321 acgatcgggg tcctcttcgg gatggaggac acgtttccct gggccctctg ccacgagatc
     1381 aacgagctcg cccggcgtcg tgggctcgcc gtcaagggcg agccggtccg ggtggggcac
     1441 gtctcgcagg agcaggcgtt cggctatgac gtgatcctcg accggatcag ccacgaggtg
     1501 ccgttctacc ggacgttcct caagtgcgcg gccgcccgcg gcgtccaggt cgtcaacaac
     1561 ccgttctggt ggtcggccga cgacaaattc ttcgacaacg tcgtggcccg tgcggtgggc
     1621 gtcgccgtcc cgcgcaccgt cctcctgccc cacaaggagc atccgccgaa cacgacggac
     1681 gcctccttcc ggaacatggt cctcgtcgac tgggacgagg tcttccgcta cctcggcttc
     1741 ccgatcttcc tcaagccggc ctacggcggc ggctggaagg acgtctggaa ggtccacagc
     1801 cgggaggagc tcttctcggc ctacgaccag acgcgcgacc tgacgatgat ggcccaggag
     1861 gcgatcgagt tcgaggacta ctaccgctgc tgggtcgccg gccggcggaa ggtgaggatc
     1921 atgccgtacg cgccgaagga gccgcacgag gcgcgctact cggccgtcgc cgggaaggtc
     1981 gtcccggacg agatggcgct ccgggtcacg aaggacgcgc ttgccctctg cgacgcgctc
     2041 ggctacgaca tgaacaccgt cgagttcgcc gtgagggacg ggatccccta cgcgatcgac
     2101 ttcatgaact gcgccccgga cgccgacctc cactcggtcg gcgaggagag cttccgctgg
     2161 atcgtcacgg agatggccga gaccctggtc gagcgggccc tccacccgat cccgtgggag
     2221 ccgacgggaa gctggccgaa ggcgctcggg ctcctgccgc gctgaagccc ggcggaccgg
     2281 gcgcctccgg gctcactccc tgggaagcgg gctcccttcg agatagaggt cgatgaccgc
     2341 gagggcgtcc ttccgcggcc cctcgttggg ccagagcgcg acggccgccc ggagctcgcg
     2401 cgcctcttcc tcgaagcgcc tcggcccgaa ccgctcggcg gcgaggaggg cgccccacga
     2461 cagccccttc acgtgcgtga tccgcccgat cgacaggtcc ttcgcctcgg cgcggtagtc
     2521 ggcggccagc cggacgaggg ccgtcctcac gagcggctcc cagtccggct cgggggtccc
     2581 cgccatcgcg atgaagttca ggacgtgctc gcgctcgagc ggctccggtt gctcgaggcg
     2641 tgcggcgagg cgtgccgtga ggtccgggag cgcccggatg cgctcgagag cctgctggtg
     2701 gtgctcctcg ctcttgtcga tgaagaggtg cgagaggaag acctcgatcg gagcgtcgtc
     2761 ggggagggcg gcgagctcgg cggcctgctt ctcgcggaga gcgtccatct cccgccggtt
     2821 ccgcgcctcc cagtcctcgt tcgcctgccg cgcctcctcc acgacccggg ccttctccgc
     2881 cgaagtcatc gcgaggaaga cgagcccgcc tgcgaaggag agggccgcga gaccggcgag
     2941 agcgacgccg gccggacggg gccagcgcgc ggcggagacc gcggccggct cggaccagag
     3001 gagggcgccc aggaacgcga cgaccgcgag cgggaggacg agccctccgg cgaagccgag
     3061 gacggttcgc gaggagtacc gcacctccag cgagaagatc gccgcggccg tcgagaggat
     3121 cccgagcccg acgacggtcg cgaggacggc gagcgtcggg accccgggcg accggctcac
     3181 ccagtcgaat cccccgtgcc cgacgccggc gagcgccgcg acgaggacga ggacccaggc
     3241 cgccacaggg aggaagagga cgacgacgag gcccatcgca tccgggccgc tcggcggccg
     3301 gagcgtgaac atcttcgtcg tcagggcgag agcgaacgtg aagaggacgg cgaggccgag
     3361 ggcggcagtg ccgaggcccg gaaggagcgt tttcatggct ggtgggcgcg gatggtagcc
     3421 gagaagcgcc cgggagcgtc agacgagccg ttcgcggagg agcgcggcgt gcctccggct
     3481 cacctcgacc ggctcgggga ggccgcgcag ccggacccgg aagcccgcct tgaaccaggg
     3541 ctcgatccgt tccacgtgct cgacgttcac gatcgtcgag cggtggaccc ggaggaagtg
     3601 ccgcggcggg aggagctcct cccactcctt caggctccgt cccacgagcg tcgtccggcc
     3661 gtcggcggtc accgcctcgg aggagtcgcg cgcggccctc aggcagacga tctgcctcac
     3721 cctcacgacc cggtagtgcg tccccgaggg gacgatgagc gggtcgtcga ggtggagcgg
     3781 gcggcccggc gcgctcgcgg ccggggcccc gtcgaggagc ttccgcacgg cggcggcgag
     3841 ccgttccggc gggaccggct tcatcaggta gtcgagggcg ttcgcctcga aggcgcggag
     3901 ggcgtactcg ctgtaggccg tcacgaagac gacgcgcgtc ccggcgggga ccttctcgac
     3961 gaggtcgaag ccgctcgccc cgggcatccg gatgtcgagg aagacgacgt cgggcgagag
     4021 ccgcgcgatc gccgcttcgg cctccgcgac gtcccccgcc tcgcccgcga cctcgatctc
     4081 ggggtgggcc gcgagcatcc ggcggagctc ggcgcgcgcg agccgctcgt cgtcgacgag
     4141 gagggcggtg aggcgcgtct tcacgccccc tccgccggga cctcgagcct cgcgacgacc
     4201 cgctcgccct cctgcgcgat cgtgaacgcg tgccggcccg ggaagtggtg ggcgagccgc
     4261 tcgcggacgt tcgcgaggcc cgtccccgtc ccctcgcacg tgccgttgct gccgagcgtc
     4321 ccggtgttcg tcacctcgac gacgagccgg ccggccgcga ggcgcgcgga gatcccgacg
     4381 cggagcggca tcgggctcgt ctccatcccg tacttgaccg cgttctcgac gagcgggtgg
     4441 acgaggaagc cggggaggcg cgcggccttc gccgactcct cgacctccca ggtcacctcg
     4501 agccgctcct cgaaccggat ctcctggatc gcgaggtagc tccggatcgc ctcgagctcg
     4561 gcctcgagcg ggatctcgcc cgagcgggcg tggaggagcg agtagcggaa gaactcggag
     4621 agctcggtca ccatctgccg cgccttggcc cgatcctcct cgatgagcgc ccggatcgag
     4681 ttgagcgcgt tgaagaggaa gtgggggttc acctggtagc ggagcatctc caggcgcgcc
     4741 tggctcgcga gggcgtcggc ccggagggcc cgctccgtct cggcgtcgag ccgggcgcgg
     4801 taggtgaacg cgaggtagag cgcgctccag gccaggagcg tgatgcccga gttcaggccg
     4861 ctcttgaagg cgccgggcca gaccggcttc tccggccaga gaaggcgcga ggcggccgcc
     4921 gaccagacga cgccgaagag gagggaggcg gccacggcga cgaggacgag ccggccggtc
     4981 ctcggcctct cggggaggag atgccggtag accgcgaaga ggccgaggct cgtcgcgaac
     5041 ccgagcgacg ccaggaccgc cttcgccggg aggaactcga ggagcgcccg gaacgagggc
     5101 tcggcggcgt aggcgaggcc ggagagccag aggatgagga agtagcttcc ccagccggcg
     5161 agctgggcga tccagaaggg acggggagga agcttcatgg cgcggcgggc ggaacggctt
     5221 cagggggccg tcttcgaggt gccggtgtag gtcttctggg cgatcttcca ctccccgtcg
     5281 atcttcagga gggagaggta gtcgacgaag aagaccttcg ggtagtcgag ctcgaccttc
     5341 gcgagagcgg cgtcgcccgc cacgtcgatg gagacgatcc ggcgcttccg ctgcgcctcg
     5401 tcgggggcgg gcttgccggc ggcgcgggcg acgaagtcgg gcgccgacat cgagacgagc
     5461 ttcccgtcgc gcacgcccgt gaccatcgcg atggggtgga acgccttccg ccactcctct
     5521 ccgtcgccgg tcgcgtgtcc ccgcaggtag gcctcgacgg gcgcgcggac ggccgggtcg
     5581 gcctcgccgg gaggaaggga cgtcgcgatg agcgggacga ggaggagcgc gaggaggacg
     5641 atggcggcga gcagggcgag cgttctcttc atgaaggcct cccgcgcgcc cggaaggcgc
     5701 ggcgcttccg ttcggggccc ggtcgtcgtt tccggcgtcg cgatcatgcg ggaaatctgt
     5761 cccggcgacg acgggaggtc acgccggagc ctgcgagcgg cgcgaaccgt cgacgagcgg
     5821 tcgattccgg cgacgagcgg aggggaggag aatgccccgg tcgcggacgg acgcgacgga
     5881 ggtgacggcg tgaggggatt cggcgtgctc gtggcggtcc tcgccctcgg agcgctcgcg
     5941 gcggcgccgg agggggagcg ggcggacctc gtcctccgga acggcaagct cctcacgatg
     6001 gacccggagc gccccgtcgc gtcggccctc gcgatcgcgg gggaccggat cgtcgcggtt
     6061 ggaagcgacg ccgagatcga ccgcctccgc ggcgaaggga cgcgcgtgat cgacctcgcc
     6121 gggaaggcgg cgatgccggg gttcatcgac gggcacgcgc acttcaccgg gatcggcgac
     6181 gcccggattg ccctcgacct gcgaggagcg gcgagctggg acgaggtcgt cgcacgcgtc
     6241 gcggccgcgg cgaagaaggc ccggccgggc gagtggatcc tcggccgcgg ctggcaccag
     6301 aaggagtggg cctctgcgcc ttcgccgaac gtcgaggggt ttccgctcca cgcctcgctc
     6361 agcgcggcga cgccggagaa cccggtcctc ctctcgcacg cgagcggcca cgccgccttc
     6421 gcgaacgccg tcgcgctcga gcgcggcggc gtggggcgcg acacgaaggc gccggacgga
     6481 ggcgagatcc tccgcgacgc ctccggcgcg ccgacgggcc tcctccgcga gacggccgag
     6541 gacctcgtct cggcggcgct gcagaaggac ctcgcgcggc ggacgccggc ggaggtcacc
     6601 gcggacctcg acaggcggat cgacctcgcc gtggaggagt gcctccgaaa ggggatcacc
     6661 tcgatccacg acgcggggag ctccctcgac gtcgtcgcgc gctaccggca gcgggccgag
     6721 gccgggacgc tcggcgtccg cgtctacgcg atgctctcct cctcgaacga ggagctcgag
     6781 cgggaagcgg cgcgggtccg cgtgatcggc gccgggcgga accacctcac cgtccgggcc
     6841 gtgaagaggc tcgccgacgg ggcgctcggc tcccgcggcg cctggcttct cgccccgtac
     6901 gccgacgctc cggggaccgg gctctgcacc tacccgatga tggacctcga gcggacggcc
     6961 gagatcgcgg cggcgaacgg cttccagctc gggacgcacg cgatcggcga ccgcgcgaac
     7021 cgcgaggtcc tcgacgtcta cgcgcgcgtc ctcgcacgcc acccgagaaa ggacctccgc
     7081 ttccggatcg agcatgccca gcacctcgac ccggccgacg tcccgcgctt cgggaagctc
     7141 ggcgtcctcg cgatgatgca ggggatccac tgcccctccg acgcgccgtt cgtgccggcg
     7201 cgcctcggcg cggcgcgcgc acgggacggg gcctacccgt ggcggcgcct cctcgacgcc
     7261 ggggcgcgga tcggcaacgg caccgacgcc ccggtcgagg acgtcgaccc gatcgcctcg
     7321 ctccacgcct ccgtcacgcg gcagccgaag aaggggccgg cgttcttccc ggagcagcgg
     7381 atgacgcgga tggaggcgct ccgctcctac accgtcgcga acgcctacgc cgccttcgaa
     7441 gaggggatca aggggaccct cgcaccgggg atgctcgccg acgtcgtcgt cctctcgaag
     7501 gacctctcga ccgtccccga cgcggagatc ccgtcgacgc ggatcctcgc gaccgtcgtc
     7561 ggcgggcagg tggtcttcga agcgtcgcga tgaggcggct ggacgcggcg tgggccgcgg
     7621 cccgggcccg ggcgagggcc cgggccttcc ctccggggag cgtgaccttc cacctcgggg
     7681 cgtggtcgcg cggccggctc gcggaggacg aggccgaggc ggcgagggcc ctcctcgagc
     7741 atcacggctt ctcccgcctg ctcgtcctcc acggctccgc gctcgaggtg gacggcgcgg
     7801 tcgtcctcgt cgtcggaccg cgaggcatcg ggaagtcgac cgcctgccgg gagctcgtgc
     7861 gccacggtag ggggaagctc ctcgaggacg gtctcctcgt cgtcggggag cacgggggag
     7921 cctggcggct cgtcgagacg ggcactctcc ccgtccttcg tcggacggcc cgcctcaagg
     7981 ccgggccgcg ccgccttctc ccgcgcgtcg agggcatgcc gcacccgggg gagcggtggg
     8041 ccgagggcct tcgccggcgc cttcaccgcg cgctcgacga gcaggctttc cgcgcggcgg
     8101 tgctcctcgc cggcggtcgt ccggccggat cggtgggttg cctccgcacg gtaggtcggg
     8161 tcgtcgtcgc cgaggagccg ttcaccccgt ccggctcctt cgagatcgac gggcgcggga
     8221 tgacggaggt cccggacgtg gcctcccgcg ttccggcgag ctgcggcgtc cggcgcctcg
     8281 cggcgacggg gccgcgcgcc gaggtcgtcg cgcgcctcgt cgccgcgatg cgcggctgac
     8341 gcctcggcca cggggcgacc gtacggctcc gcgtcggctc cccccggcgg ttccccttct
     8401 cctagactcc cgaccgaagt cccgacccgg aggcgaccca tggcgcgaac gtcccgcgac
     8461 ccggagacgt cccttctcct cgacctcctc gcggaggcgt acgagaggag gtcgtggcac
     8521 ggcacgaacc tccgcggatc gttccgcggg atgacagccg ccgaggcggc ctggcggccg
     8581 gccgaggggc ggccgaacgc ctgggagctc gccctccacg ccgcctactg gaagtacgcc
     8641 gtgcggcgga agctgacggg ggagaagcgc ggctccttcc cgctcgaagg gagcaactgg
     8701 ttccggcggc ccgaaggcga gcccgccgag gaagcctgga aggcggacct ctccctcctc
     8761 gccgccgagc accgggcgct cgtcgcggcc gtcgcggagc gcctcccggg gctcacgggg
     8821 ccggcgcgcg cgaaggcgct ccacctcgtc cgcggcgtcg cggcgcacga cctctaccac
     8881 tgcggacagg tgcaggctct gaagaagctc cgaccgaagg gatgacgggc ggcttcttcc
     8941 ccccgacgcg cgcctcggtc gtcgacgcgc tccggagcgg cgacgccgcc gagcgcgagc
     9001 gtgccctcga gaccctcgtc gcggcctact ggcggccggt ctaccggcac gtgcggcgga
     9061 agtgggggaa ggggcacgag gaggccgccg acctgacgca ggacttcttc gccgagctcc
     9121 tgtcgcgcca cctcctcgcc cgcttcgacc cggggaaggc gaggctccgg acctacctga
     9181 ggctctgcgt cgacggcctc gcggcgaacg cggcgaaggc cgcgtcgcgg cagaagcgcg
     9241 gcggcggggc agcgcccctc tcgctcgact tcgaaggggc gcgggaggag ctcgagcggg
     9301 ccgcgcccgc cgcggacgag accccggagc gggcgttcga aagggagtgg gtgcggagcg
     9361 tcctcgcgct cgccctcgaa cggctcccgt ccgcgctcga ggggcgcgga aaagggatcg
     9421 ccttcgcgct cttcgagctc tgcgagctcg gggcggaggc cgaggtgccg tcctacgccg
     9481 agctcgcggc gcgattcggc gtctccgtct ccgacgtcac gaacggcctc tccctcgccc
     9541 gccgggagct ccggcggatc gtcctcgacc tcctccgcga gctgaccggg agcgaggagg
     9601 agctccgcct cgaggcgcgc gccgtcctcg ggatcgacgc gccgtgaccg cgctccccga
     9661 cgccgccctc tcccggctcg tgcgcgccct ccgcacgccg gacctgtcgg aaacgcgcta
     9721 cctcctcggc cgggaggtcg ggaggggtgg catggggagc gtctgggagg cggacgaccc
     9781 ggagctcggg cggagcgtcg cgctgaaggt cctcgacctc cccgactccg cgggcgacct
     9841 ctccgcgcgc ctgagacgcg aggcgcgcgt cctcgcgcgg ctcgagcacc cgggcgtcgt
     9901 cccggtccat gacgtcggga cgctcccgga cgggcgcccc tggtacgcga tgaagctcgt
     9961 ccgcggagag aggctcgacg cggcggcggc gaggctctcg acgctgcccg aacggctccg
    10021 cctcttcctg cgtgtctgcg agcccgtcgc ctttgcccac gcgcgcgggg tcgtccaccg
    10081 cgacctgaag ccctcgaacg tcatggtcgg cccgttcggc gaggtcctcg tcctcgactg
    10141 ggggatcgcg cgtctcctcg aggaggaggc gtcggaaggg agaccggggc cgccggccgc
    10201 ccccccgacg gagccgcggg agccgcgcgc cgcggccgcc ccgggcaccg ccgccgggac
    10261 ggtcctcggg acgcccggct acatggcgcc ggagcaggcg cgaggcgacg gcgacgtcga
    10321 cgggcgggcc gacgtctttg ccctcggcgt cctcctccgg gagctcgccg gtccggaagc
    10381 gctccgcgcc cggcgcctcg cggcgatcgt cgcgaaggcg acggcggagc ggcccgcgga
    10441 gcgctacggg ggcgctccgg cgctcgcggc cgacgtggtc cgctacctcg acggcgagcc
    10501 ggtgacggcg taccgcgagc gtccctggga gcgggccgcc cgcttcgccg tgcggcaccg
    10561 ggtcgcgctc ctcctcgtcc tggcctacct cgcggcgcgg gccgcgatcc tcgtcctcta
    10621 ccggcgataa aggaaacggc ggcgccgttg aacgggaaga aggaagggag aacgacgatg
    10681 aacaagctgc tcctggggct cctcctcggc ggagggctcg gcgtcctcga cgggctgacg
    10741 gcgtggttca cgccggaggt ccggaaggac atcctcggga tcgtgatggg gtcgaccttc
    10801 aaggggctcg tggcgggcct cctgatcggc ttcttctcga agaaggtcgc ctccgtcccg
    10861 gcgacggtcg tcttcggggt gatcgtgagc ggcttcttcg cgtacctcgt cgcggcgcag
    10921 atggggaagt actacttcga ggtgatgctc cccggcgccc tcgtcggcct cgtcacgggc
    10981 tacgtcacgg cccggtacgg gaagggcggg ccggccggaa acccggaacg ccgcctcgcg
    11041 tagaacaccg gaaccacaca ccgggtgagc accgtccgcg ggatcgcccc gcggccggga
    11101 gagatgcccc ttcggggcgg acacgaaagg agacgaccat ggccggaaag gtgaatccca
    11161 ttcccgaggg gtacggaacc gtcaccccct acctctgcgt caagggcgcc gcggcggcga
    11221 tcgagctctg gaagaaggcg ctcggcgccg aggagctctt ccgaatgccc ggccccggcg
    11281 acagggtgat gcacgccgag gtgaagatcg gcacctcgat cgtcatgctc tccgacgagt
    11341 tccccgactt cggccagctc gggccgacgt cgaggggcgg gtcgacgtgc acgatgatga
    11401 tgtacgtccc ggactgcgac gccgcctaca agcgggccgt cgaggccggc tgcaccccgg
    11461 tgtcggagcc gaaggacgag ttctggggcg accgcttcgc gaaggtgacc gacccgttcg
    11521 ggcaccagtg ggcgttcgcc acgcacgtcg aggacgtctc gccggaggag atggagcggc
    11581 gggcggcggc gatgttcgag aagggctgct gaggccgggg cggcggggcc ggggtaccgg
    11641 ctccgccgcc gttcgggcgg cgccggacgg gctccttccg tgttagcttc gttcacttcc
    11701 gagagaagga gacgccctca gatgaagaag cccctcggcc tcgcggccct ggtcctcctg
    11761 gccctcgtcc tcgccgcccc gcccgccgcg gcggacgccc tcagcctgga gctggggctc
    11821 cacggcggcc tcgtgaacag caaggacggc gaagggggga gcttcctcgg cggcgcacag
    11881 gcgcgcgtca acctcttctg gatcgtcgcg gccgaggccc gcgcctccta ctacaccgac
    11941 agctacgacc tcgggcagat cggctccgtc gacgtcgaga acatcccctt ccaagtctcc
    12001 gggatgatct acccgatcaa gctcccgaag gtcggcgtct acctcctcgg cggagggacc
    12061 tacagcaacc tgaaggtgaa ggggacgagc gtcgtccagg gcgaagtgac cgagaagggg
    12121 tggtcgaccc acgtcggcgc cgggatcgac atcaaggtcg gcgagaagac gacgctgaac
    12181 ggcgacgtaa gggccgtctt cctcgacgcc aacagcgtcg acgagctctt cgacagcgtg
    12241 aagtcgagct acaagggcga cttctggacg gtcaccctgg ggctgaacta caagctcttc
    12301 tgactccgtc tccggcgggc cgggggcgac cccggcccga gcgcggcgcc cgagcgcttc
    12361 cggggcggcg ccctgctagc atcggctcac cgaaccgatg cctgccgcca gcctgctcgc
    12421 cctcctcgac gacatcgcga ccgtcctcga cgatgtcgcg atcctgacga aggtcgccgc
    12481 acggaaggcg accgccgtcg ccgacgacgt cgccgtcctc tcgaaggtcg cggcgcagaa
    12541 gacggcgggg gtcctcggcg acgacctcgc gctgaacgcc gagcaggtga ccggcgtccg
    12601 cgcggagcgt gagctccccg tcgtctgggg cgtcttcaag ggctccctcg tcaacaaggc
    12661 gatcctcgtt cccgcggccc tcgcgatcag cgccgtcgcg ccgtgggcgg tgacgccgct
    12721 cctgatggtc ggcgggctct acctctgctt cgaggggttc gagaaggtcg cgcacaagct
    12781 cctccaccgg aagggagagg acgaggtccg ccacgccgag ctcgccgagg cgctcctgaa
    12841 gaagaacgtc gacctcgcgg cgctcgagaa ggagaagatc aagggggcgg tccggaccga
    12901 cttcgtcctc tcggccgaga tcatcacgat cgcgctcggc atcgtcgcga acgtcccgtt
    12961 cggcacgcgc gtcctcgtcc tgacggccgt cgggctcgcg atgacggtcg gcgtctacgg
    13021 cctcgtcgcg gggatcgtga agctcgacga cctcggcctc tacctccggc agaaggaagg
    13081 ggcgtcgcgc ctcgcgcgcc tccggcgctc gctcgggggc gggatcctct cggccgcgcc
    13141 gtggctcatg cggctcctcg cggtcgtcgg caccgcggcg atgttcctcg tcggaggagg
    13201 catcctcgtg cacggtctcc cgccgctcca ccacctcgcc gaggggctcg tcgctcgcgg
    13261 cggcctcgtg ggcgggatcg gcccggggct cctcggcggg ctcgtcgggg tcgtcgccgg
    13321 cggccttgcg gtcggggtct tgacggccgg gggacggctc ttccgccgca aacgctgaga
    13381 ggcggaaggc gcggcccttc ttccgcgccc cccgccccgg cccgggacta ccgcgccgcc
    13441 ttcagctcca ggagcgccgt cgtgatgagc ggcgcgacga tcgtcgcgtc cgactcgatg
    13501 acgaacatcg gcgtctcctc cgtcagcttg tcccaggtga tcttctcgtt cggggtcgcg
    13561 cccgagtagg agccgtacga cgtcgtcgag tcggagatct ggcagaagta ggcccagggc
    13621 ttcaccggct cctgcaggtc gtacttgatc gacgggacga cgcagatcgg gaagtccccc
    13681 gcgatgccgc cgccgatctg gaagaacccg acgccggccc cttccgaaag cgtccggtac
    13741 tggtcgtaga aggcggccat gtactcgatc cccgacttca cgatgctcgg cgagcactcg
    13801 cccgtcttca cgtacgacgc gaagatgttc ccgaacgtgg agtcctcgtg gcccgggacg
    13861 acgatcggca ggccggcgcg cgcggcggcg aggagccagc actcgtcggg gttcccctcg
    13921 tagaggtcgg cggggatcgt cgtgacgagc tcgtagaaat actcgtgcca gaaccggcgc
    13981 tcgccgcgct cggtggccct cttccacatc gggacgacgt acttctcgac ggcgcggaag
    14041 gcctcgtcct cggggatgct cgtgtcggtg acgcgccgca tccgatcctc gaggatccgc
    14101 gtgtcgtcct gcttcgtcag gtagcggtag tcggggaagt ccttgtagct ccggtgcgcg
    14161 acgagccgga agagcgactc ctcgaggttc gcgccggtga ccgacagccc gtggatcagg
    14221 cccgcgcgga tcgccggggc catcgtgatc ccgagctgcg ccgacgacat cgcgccggcc
    14281 acggcccaga acatcttccc gccggcctcc acgtgcttcc agtaggcgag catcgcgtcc
    14341 ttcgtggcgc gggagttgaa gttgcggtag ttcgcgagga tgaagtcgtg gatgggtgtc
    14401 gcgctcgtca tcggctctcc ctcgttcggt tccgggcggt gagcgtagcc cgactttcgg
    14461 gacggctcaa cgcggttcgg ggcggccggg cgacggcccg gccgccccga aggcgtcgcg
    14521 gcttccccgg gccggccgcg atcagcgatc cggagccttc tccgcggtca tccggtgctc
    14581 gccgccgcga cgctggatcg tgacggcgta gcgctctccg ctcttcagcc ggacgtcaca
    14641 gaccgacgag ccgtcgtcgg ggacgacccg ggaggggcag tcgaccgaac tcagctccag
    14701 cgagcgcgac gcgaacgact tccggatcag ccaaggtgcg tcgaggatct ggcacgtgaa
    14761 gtcgttccgc tcgaagtcgt ctctgcggcc gctctgctcg atccggacgg ggacctcgcc
    14821 cccggaggcg agatgggcgg tcccgaggac ggcgtccccg actttctcga gcttctcgag
    14881 gggcccggcc aggcgagcca cgggctcgcc gaggagctcc gaggccttcc cgcgcagcat
    14941 cgcctcgagc acggaggcct ggagggccga gccggcggcg gggtagatcc ggaacgccga
    15001 gactctcggg acctctccgt cggactcgat tcggaaccgg gcgacgcccg ggcctttctc
    15061 ggaatcgagg tcccagacga gctcggaatg ccggacctcg gggtccggcg tcgagcggac
    15121 cgacacgctt cggacagaaa cggaaccgag cgcgcccatc gagtcccggg ccggcccgag
    15181 ctgcgcctcc agcgcggcga ggggcagctc cgagcggagc gccgggtgca gggccgcgtg
    15241 gacctccttc caggcggcgc ggcggtacgc ctcgagaaac gcgcggcaag gaggctccac
    15301 gagggcgcgc tcctccggct cgagcctcga cgcggggaag gagacgacga cgatcgtcgc
    15361 ctgcccgtcg ccgtcgaccg cgagcgtgta gcgtgtcttc tcccccttct cgtccaccgc
    15421 gtcgcagtcg aacgaccctc ccggcgggac gggcttcggg acgtcgcacg agaaagtcga
    15481 gaagccgcgg ccgttggcct tcctgtacgc cggctccacc aggggacgcg tgagggcgga
    15541 gacctcggcg gacagcgact tcccggcggg ctccgtcggg gtcggcgcgc ctgaggcgag
    15601 gatcggaagg gcgagcagca gggacggcag aacgtgcatg gagacctcct cgacgagtgc
    15661 gccgatccgg cttcgaggcc gggagctttc aatgcggccg gccccggcct cgggccggct
    15721 ggatcaggtt gacggagcga tcacgtccag ggcggggcgt ccctacagga ggacagggag
    15781 gaccgagaag gctccgaaaa gcacggcaga gacgagccag gccacggtga actcgttctc
    15841 gacgcccggc gtcgggtgga accaccagct gagcctcgag ggtctcttcc gaaagaccca
    15901 gacgagcgcg gatcgaaggg acacgacgag aaacatgagg atcatcagcg atccccacat
    15961 ccagccctcg aaggcgtcgg cgcgcagggc cagcacggag agcaggacga cagccgccgc
    16021 cgcgtagccg tacgcgatcc ttctcccgac gtctcggggc ccgtacgcga ggcggggcgc
    16081 catctctctc tccggaggcg tgcagcttag tacgggcgcc ggccgcgccg tccggggatc
    16141 gggagaaaga tccgctctgc tgcggtgcac accggagagg cggggaggtc ctccggcccg
    16201 aggcgagtcg accgggaagc ccgggctccc ggacgcctac gtcgtgcggc gctccgtttc
    16261 ccgctccccg tgacgcgccg tcaggagcgc ggcggtggcg aggccgccaa ggacggcgac
    16321 gacgaggagg gcggcgagct ggatccagcc tccgaccgcg gccggccgga agaacgcggc
    16381 cgaggcggcg gcgaggtcga agccggcggc cgggtccttg cggtacgcct tgaccgcgac
    16441 gaggccgatc atccagaggc cgctcgcgac gagcgtgacg gccgctccga ggaggctgcg
    16501 ccgggcgatc tggcgggacg tcaggcggcg ccgcggctcc gccgtctcga cggtgaagac
    16561 cggctcccgc gtcccgagcg gtgcgaatcg ctgcagcagc aggaggcccg gcacgccggc
    16621 ggcgatcgtg aaccagaaga acgtccgcca cccggcggcg tcgacgatga ggccggtgat
    16681 cggcccgccg aggatgcggg ggaggccgaa gagcgacgag aagagggcgt actgcgtcgc
    16741 cgagaagcgc ttctcggtca tccgcaggag aagcacgccg aaggccccca tgccgaggcc
    16801 gctcgtgacc atctcgaacg ccatcgcgcc gtacatcacg gggcggctgc ccggctggta
    16861 ggtgatcagg acgtagccga tgttcgagaa gatctggaag aagccgaaga tccagagcgc
    16921 gtggccgagg ccgatccggg tgcaggcgac gccgccgagg aacgtgccgg cgatcgtcag
    16981 cgcgacgccg gcggtcccga ggacgaagcc gcggtcgaag ccgccgtacc ccatgtcgat
    17041 gaggaacggc cgcaggagcg actgagagag ggcgtcggcg agcttgtaga ggacgacgaa
    17101 aacgaggatc tccaccgcgc ggtggcgcga gaggcactcg aggaacggca gccagaccgc
    17161 gtcgcgcagc gtcttcggcg cggcggcctc ttcctccggc tccggcgagc gccacgtgac
    17221 gaggaggagc ggcaggtaga cgaggcccag gaggaggttg acggcgcccc acccgaggct
    17281 cgcagcagcc cagatcgcga gcccgcccga gagggtcatg gcggcccggt agacggccgt
    17341 tcggaggccg accgcgacgc cgtgctcctc cttccggagg acg
//
LOCUS       DAOTEZ010000030        17266 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2241_length_17266_cov_5.883040, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000030 DAOTEZ010000000
VERSION     DAOTEZ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17266
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2241_length_17266_cov_5.883040"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="P5164_02695"
     CDS             complement(<1..816)
                     /locus_tag="P5164_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019496541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HRY42832.1"
                     /translation="MLPDLPAPGQLARVRARSYLVEEVVPPTRPGEQTLVRLSCLDDD
                     AQGEELSVLWEREVDAAASGGSGWTTVGKNGFDPTKLFSAYLHTLRWNGVTAADPRLM
                     QAPFRAGIQVKAYQLEPLRKALRMPRVGLFIADDVGLGKTIEAGLIVRELLMRQRVKK
                     IVVACPASVVLQWRDELDRRFGLTFAVFDRDFVLARRRERGYGVNPFTTHSRFVLSHS
                     LLRDEAYAGPLRDWLGDFEPGSLLILDEAHNAAPASGAKYAIDSALTKAVRDVV"
     gene            complement(980..1426)
                     /locus_tag="P5164_02700"
     CDS             complement(980..1426)
                     /locus_tag="P5164_02700"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRY42833.1"
                     /translation="MRRAALLLALLATLPLAAVETDQQGRAHEWAGPSAGYTVVDFAA
                     SWCTPCWSVLPRLEKFAAEHPEIRVVAVSVDDKVSGRDLLVSKLKLTIPVLWDEKHRI
                     AEHYRPDGMPHTVVLDPSGKVVYRHTGSAQKEWDAMVAFLANAAKR"
     gene            complement(1426..2610)
                     /locus_tag="P5164_02705"
     CDS             complement(1426..2610)
                     /locus_tag="P5164_02705"
                     /inference="COORDINATES: protein motif:HMM:NF023516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HRY42834.1"
                     /translation="MQLSRLARPLLAAALSAAASAAYAQAPPDGVLDADFLFGYYDQD
                     GDRSPVTGGVGTEKLHVYSPVLQLAWRASESLTLSADVGIDQVSSASMGYIQAELSSA
                     SIPASDQRAFGTLKAKKKWRGSTWGLTVGAAKEYDYRSFSYGIDWGLELNQANTALSA
                     SVRRFDDAIDLIGIDGHGYQGPGQPLTTGSGDRTTTDVTVGVSQTLGRRTVGSLELFL
                     SSQDGVLSTPYHEVVLGPFEPGAVRVAERLPDSRLRKALSLRVNHSFSDKLVLRTGYR
                     FYTDDWGISAHTIELEPNFRLPSEREMWLFPILRYSTQAGADHFGAPGSFTGAERYYS
                     SDWDLAETSTAKYGLGYTVNTRPGQTWLGIFRRFEARAAVYDRNDGLTAFSTSFSVGW
                     TY"
     gene            complement(2601..2822)
                     /locus_tag="P5164_02710"
     CDS             complement(2601..2822)
                     /locus_tag="P5164_02710"
                     /inference="COORDINATES: protein motif:HMM:NF025452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HRY42835.1"
                     /translation="MKRRLPVILALTGLSALLPGCTTVQPWERGALSTEPMKLDDGRA
                     HRFERAAETYREGAVGGAGGKSGGGCGCS"
     gene            complement(2819..3850)
                     /locus_tag="P5164_02715"
     CDS             complement(2819..3850)
                     /locus_tag="P5164_02715"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRY42836.1"
                     /translation="MVRATRRTGAGCIGGAVLLLSLAVRGPVAAATLKDPRGAMGSKF
                     EITAVHADPEKGRAAIDAAYAEIERIEEFLSEWRDSSKVSEINRQAGIAPVEVPKELL
                     HLVRRSLKVSTLTDGAFDVTFHTVGRLWNFKSHNAPVPSEEAIRAALADAGHRHVILD
                     EAKSTVFLDRKGTRIGFGGIGKGYAANRAVFVLKEHGVTGGVVNAGGDLVVFGTQESG
                     RPWRIGIANPLEKSRTFAWLETTEQAVVTSGDYENYIELGGRRYSHILDPRTGWPVTE
                     VRSVTIVCPDGEVADALATGVSVLGVEKGLALVNRLNGVEAMLVDANGKIHFSKGLET
                     MLTKEGGDR"
     gene            3942..5297
                     /locus_tag="P5164_02720"
     CDS             3942..5297
                     /locus_tag="P5164_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRY42837.1"
                     /translation="MHYIAMELVSGRTLASLIHEKRTDLRTLAGYLAQAADGLAKAHA
                     AGIVHRDFKPANVMVSTDGFTKVLDFGLAKLTETPSRAPGLSDAPTMESPGSAAGSVL
                     GTAGYMSPEQVAGRPVDHRSDVFSFGCVLYEAATGRRPFEAPTAVEAMHRILNEKPVP
                     VEELNPRVPAELRRIIRRCLAKGPDQRVQSMKDLALQLREIVEEWDSLPASGSSATSS
                     AGVAAPGRKARPLVFVAAGAGFLVVGALLALGLRGRQGAEPEGAQAFQTMRMSTQTSR
                     GDVFDAALSRDGRYLAYLHGRAGLTGLRVRQVATGSDVEVVPPSEAGLSRVAFSPDGN
                     YVYYLARRPEDRRYQALFVVPSLGGPPRERLFDVDSKVTFSPDGKRLACWRGVPQKQE
                     NVLLSFDLESSRETVLATLGADEPMVTAPAGPPWSSRTRSSRASPGSPTAAASSGRAS
                     A"
     gene            5423..6403
                     /locus_tag="P5164_02725"
     CDS             5423..6403
                     /locus_tag="P5164_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRY42838.1"
                     /translation="MRTSRLANLWIVDTSDAAARKLTSVTSPEGSPWNMDVAGADQVV
                     YEVPLDGTLRIWSVDAAGGEPRSLTTDSAHTFNTKAAAGVVLFDRLDDTGVHIWRMAA
                     DGTGRRQVTTGSGEQVRHLSPDGRFVAFERWDDAGSIHLFDLESGKASKLFERAADVF
                     GFSPDSKRFLVTRLEPDEKGQPRPVWQALSVEGNEAPATFPGPAGALGAVWTPDSRGV
                     SFRRRNDPAWNVFRQGLGEGEPVQVTRFTEGRLMGHFWSPDGRKLAVTLRTREGVDLW
                     VTEADGTRPVRVTRLPELEVFGVRWLPDSSRLVVSAGTRSQDAVLIRGFR"
     gene            complement(6400..7086)
                     /locus_tag="P5164_02730"
     CDS             complement(6400..7086)
                     /locus_tag="P5164_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRY42839.1"
                     /translation="MQRMESVFGPGIGESQRRLLGLIKRSGECTLARLEAGFDLNRET
                     LRTHLKSLVAMGLVERPGVHRKGPGRPHVLYRLTAAGEALFPRREGELLGELASFLHE
                     KGRRDLLEEFFERRLARRRRELAPRVAGLRGRERLEAIAGILSEEGFVAEVVAGDGGP
                     RLRLCHCPLREMVALSDLPCRFEKRLIEGLLGVRSRRETFIPEGEHACSYAVGPLSRL
                     GRERGSAPAA"
     gene            complement(7100..8530)
                     /locus_tag="P5164_02735"
     CDS             complement(7100..8530)
                     /locus_tag="P5164_02735"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HRY42840.1"
                     /translation="MASNPIPAASSAPAGPPSASSVCPLAAGASPALLPALARVPLYP
                     RFFLAGLAVTLTAGAGWGAWLLLRIARAHDFSAPSIFEINAHGQAQIYGWMGLFIMGF
                     ALQMFPAFWGVRLSSPRLARALLPLMALAVVARTVGEARPVGPFATLAVAAGALQLAV
                     VAAFVALLAATAIQKGVRRRAADLYIGAGLVWFLLSTVLDLRHLVQTIGAPDRASLLA
                     EIAVWQLPLRDLQIHGLALSMILGVSMRVLPGLFGTPVVDERRAVRLFGPLQAMIVLE
                     VALFPLAMTSRRPALFVAVWLAWVGLAVCAALAVSSLRAFARPRFVVPRLSPLAFVRA
                     AHVWLGVSLAMLVAGPFWSRLVGIPFSHAWHGATRHAITVGFVSLMIVGVASRVAPRP
                     PGSSADAEAMPLAPFVLLNLGCTLRVTQQVLTDLTPVAFPTAGVSGLLEVSGLVWWAT
                     WIVPRLLAASRPQGGRVGVAPGAARV"
     gene            8546..9067
                     /locus_tag="P5164_02740"
     CDS             8546..9067
                     /locus_tag="P5164_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRY42841.1"
                     /translation="MGDNQTRQSREADGSRPPAPPPSRLDYHEVAPDGFAAMLGLERY
                     VRGSGLPHALLELVKVRASQINGCAYCLDMHTKDARAAGETEQRLYAVAAWHEAPFFT
                     ERERAALAWTEAVTRVGESRVPDELYEEVRRHFDGKELVDLTLAIVVINGWNRLAVSF
                     RTAAGSYRPDLAR"
     gene            9081..11414
                     /locus_tag="P5164_02745"
     CDS             9081..11414
                     /locus_tag="P5164_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013925600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRY42842.1"
                     /translation="MSPDGGSPHPSERPLSPWWRHAVILVMTVGFSLLTLVTVLTYTN
                     APPVPERVTDAAGASLFTRENVEHGQEVFLKYGLMEHGSLWGHGAYLGPDYSAEYLHR
                     LAEIARETLALERHGKPYTALAPDAAAAVAAGVSGLLKENRYDEASGTLRFSPAEASA
                     WRIQSAEWAAYFSGPTPAPGLPAQYIRDPRDLADLNSYFAWAAWATVANRPGKDYSYT
                     NNWPYEPLVGNRPSASTYLWSALSLVTLLGGLGALLFAFGRFDFLGWHGPSGAGHEHE
                     SLLARWTLTPSQKAVGLYFGVVALLFLLQALAGGALAHYRVESGAFYGFDLARFLPYN
                     LLRTYHLQLAIFWIATAWVAGGLFLAPLVGGAEPRGQRAGVIALLAALAVVVFGSLGG
                     EWLGINDRLGSLWFWLGHQGSEYLDLGRFWQLLLAAGLAGWLVLMYRALRPALRDPKR
                     SELASLFLYAAAAIPLFYLPALFFGPRTNFAVIDNWRFWIIHLWVEGFFELFATVLVA
                     VMFLVLGLVTPRTATRVVYLDAILYLAGGIVGTGHHWYFTGQGTLNMGLAACFSALEV
                     VPLTLLTLDAWDFVKLRERRCDDCHATFADRHRWTVYFLMAVGFWNFVGAGVFGFLIN
                     LPVVSYFEVGTSLTSNHGHAALFGVFGMLALAVLVFTLRSLARDEVWARAERFVRVGF
                     WGLNAGLASMMAVDLFPAGVLQLQDVLQNGYWHARRLTYLMEGTFHTLEWARAAADGL
                     FLVAGVVPLVLAVAVLLLGSRGGHAAPAGPGGGTISG"
     gene            11411..11986
                     /locus_tag="P5164_02750"
     CDS             11411..11986
                     /locus_tag="P5164_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42843.1"
                     /translation="MKTTAGLLSLALLLTGCQKTEAPAGETPKAAADAQAAVAQAPAV
                     PASPSDPKALLTDEMLGKYVVYQTAVLPVMGDTLAMGGSALQGSGGEQKEFEKQVAAD
                     PRLQRVEAATKEASAKSGLTPQTAAAIGGVVSEYVSARSMGSPEEKAKVRADFELKYG
                     TPAAEAMDRHEAELTKLQEEMLKAALGPAKK"
     gene            12068..12451
                     /locus_tag="P5164_02755"
     CDS             12068..12451
                     /locus_tag="P5164_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRY42844.1"
                     /translation="MTRVTGIGGIFFKARDPESLREWYRKHLGVPLEEWGGAVFHWQT
                     AERPEPDGMTVWNIFPATTTYFAPSPAPFMVNFRVEDLDAVLAALREEGCAVDEKVER
                     SEFGAFGWVMDPEGNRVELWQPPKS"
     gene            12749..13153
                     /locus_tag="P5164_02760"
     CDS             12749..13153
                     /locus_tag="P5164_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42845.1"
                     /translation="MKMRSLLCRVFPLVLLAAFVAAPVTADDAALFRIGHVVVVGDVR
                     LPAGDYTFRATPRGLVLVYDREMKKVQAAVLVERSPLTWAEHEKANTLSHDWAVRSLY
                     LGEHGYLFAPGPVPPDLVARCGPATTVVAQVR"
     gene            complement(13172..13519)
                     /locus_tag="P5164_02765"
     CDS             complement(13172..13519)
                     /locus_tag="P5164_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42846.1"
                     /translation="MARTKSNHERLLATVEATYRLAERSFDALHSAAKGPEEKERVRE
                     LYGAARDAYWKAVLACLEDQSGVVAAIHDELKAKNRELKRSLETLRDLASTLALLRDA
                     VRLAAALVTLAAA"
     gene            complement(13531..14523)
                     /locus_tag="P5164_02770"
     CDS             complement(13531..14523)
                     /locus_tag="P5164_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42847.1"
                     /translation="MNRPVRVSVVLLSLAALAGCTSTARPRAGSLASAGGAATRVAAD
                     SVREAAASVDRYVEAQLLLAPLTGRPDPAEETLATLRKVRSVLAARGDAMSELGAAYA
                     GLDALAAFDAADQVERGVNDLAGAVSAYREALGRPAAAPVPAFGLSKGLGAFAGARHA
                     ARLKKASAAIRESLERFVALLRTEREVHASLRRALVEGQGTAARAFAALGLARPGTLL
                     APHAAAFGLAWDEKEYDGALALLRKEPSITGRPGANREDDLRAALDRVLEHRVARRVD
                     LEGALVDETLAGLEALVLAHRSFEAKQETDLAAVHGHVAAVRALLDEYRAAAGK"
     gene            complement(14587..14964)
                     /locus_tag="P5164_02775"
     CDS             complement(14587..14964)
                     /locus_tag="P5164_02775"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRY42848.1"
                     /translation="MIGPNIRIQGELSGDEDLVVEGRVEGKISVSKGLRIGPQAQVNA
                     EVKAHHVVIAGRVVGNVVAAEKVEILPSGILEGNIRSPKIAIAEGAQFKGSVDMGGKS
                     VPASPAPASSGPAPEKKDASGKS"
     gene            complement(15109..15783)
                     /locus_tag="P5164_02780"
     CDS             complement(15109..15783)
                     /locus_tag="P5164_02780"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HRY42849.1"
                     /translation="MTAVRWAAARSEDLPRGEAWLAPAERLALSRLTVPKRRGDWLLG
                     RFAAKKVLLLAGAIAREEEAAVLAADSGAPEAHVGGRPAPFVLSITHSHGHAAAAIAP
                     AGARLGLDLERIEVRGGAFLEDWFTPSERGFVEGAPAGEAALRATLVWCSKEAVMKAL
                     REGLRIAPRDVEAAPAPGPADGSWRRFGARGPGPSEWSGWWRAEDGFVLAALADPPSG
                     PPERIG"
     gene            complement(15780..>17266)
                     /locus_tag="P5164_02785"
     CDS             complement(15780..>17266)
                     /locus_tag="P5164_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019610006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRY42850.1"
                     /translation="DPMGAMGSLAEPECRRILAGLDLAERMGVPVEWYALFAGAKIAM
                     DSGTENMDWISRVLRRLITFTQAGGEVNVVVCGINVGAQPYWNAEATMLMHTRGILVM
                     TKDGAMVLTGKTALDYSGSVSAEDNLGIGGYERVMGPNGQAQYWARDIPDACRILLRH
                     YDHTYVVPGERFPRRAETSDPIGRDVCLSPHGGEEPGAFALVGEIFSDATNPGRKRPF
                     EIRKVMAAAADQDHAPLERWAGLRDGENGVVWDAHIGGWPVCLLGFESHPVKRIGFVA
                     TDGPENWTSGTLFPQASKKIARAINAASGNRPLVVLANLSGFDGSPESMRKIQLEYGA
                     EIGRAVTNFRGPIVFTVVSRYHGGAFVVFSKTLNDNMEVAALEGTYASVIGGAPAAAV
                     VFAREVDQRTKKDPRVAALEKEMAAVSDVERRRLRSRHADLVAAVRSEKLGEVADEFD
                     RTHSVQRALAVGSLDRILPAKELRPYLVDAIERGMAKEMERIAR"
BASE COUNT         2404 a         6097 c         6366 g         2399 t
ORIGIN      
        1 gacgacgtcg cggacggcct tcgtgagggc cgagtcgatg gcgtacttcg cgcccgaggc
       61 gggggcggcg ttgtgggcct cgtcgaggat gaggaggctg ccgggctcga agtcgccgag
      121 ccagtcgcgg agcgggccgg cgtaggcctc gtcgcgcagg agcgagtgcg agaggacgaa
      181 gcgcgagtgc gtggtgaagg ggttgacgcc gtagcccctc tcgcggcggc gggcgaggac
      241 gaagtcgcgg tcgaagacgg cgaaggtgag gccgaagcgg cggtccagct cgtcgcgcca
      301 ctggaggacg acggaggccg ggcaggcgac gacgatcttc ttcacccgct gccgcatgag
      361 cagctcgcgg acgatgaggc ccgcctcgat cgtcttgccg aggcccacgt cgtcggcgat
      421 gaagaggccg acgcggggca tccggagcgc cttcctgagc ggctcgagct ggtaggcctt
      481 gacctggatg ccggcccgga agggggcctg catgaggcgc gggtcggccg cggtgacgcc
      541 gttccagcgg agggtgtgga ggtaggccga gaagagcttc gtggggtcga agccgttctt
      601 ccccaccgtc gtccagcccg agccgcccga ggcggccgcg tcgacctcgc gctcccagag
      661 aacggaaagc tcttcgcctt gcgcgtcgtc gtcgaggcag gagagtcgga cgagggtctg
      721 ctcgccgggg cgtgtcggcg ggacgacctc ctcgacgagg tacgagcgcg ccctcacgcg
      781 cgcgagctgg cccggggccg gcaggtcagg cagcacgacg ttggattgtg cggcgatatc
      841 gcccgattga aaatgggctg gactcatcag gccgcacgaa tcaaccgtgc ggcaggcacc
      901 cggacaaatg gcgtctgtgg cgtcccgtcg cggccggcgc cgcccgctcc gggacgagcg
      961 ggcgggcgcc ggcggcgcgt cagcgcttcg cggcgttcgc caggaacgcg accatggcgt
     1021 cccactcctt ctgcgccgag ccggtgtggc ggtagacgac cttgccggac gggtcgagga
     1081 cgacggtgtg aggcattccg tcgggacggt agtgctcggc gatgcggtgc ttctcgtccc
     1141 agaggacggg gatggtgagc ttcagcttcg agacgaggag gtcgcgtccg gagaccttgt
     1201 cgtccacgct gacggcgacg acgcggatct ccgggtgctc cgccgcgaac ttctcgaggc
     1261 gcgggaggac gctccaacag ggggtgcacc aggaggcggc gaagtcgacg acggtgtagc
     1321 cggccgaagg gccggcccac tcgtgcgcgc ggccctgctg gtccgtctcg accgccgcga
     1381 gcgggagcgt tgcgaggagc gccagcagga gcgccgcgcg ccgcatcagt aggtccaccc
     1441 gaccgagaag gaggtggaga aggccgtcag cccgtcgttg cggtcgtaga cggcggcgcg
     1501 cgcctcgaac ctccggaaga tcccgagcca ggtctgcccc gggcgggtgt tcacggtgta
     1561 gccgaggccg tacttggcgg tggaggtctc ggcgaggtcc cagtcggagg agtagtaccg
     1621 ctcggcgccg gtgaagctgc ccggcgcacc gaagtggtcg gcgccggcct gggtgctgta
     1681 gcggaggatc gggaagagcc acatctcgcg ctcggacggg aggcggaagt tgggctcgag
     1741 ctcgatcgtg tgcgccgaga tcccccagtc gtcggtgtag aaacggtaac ccgtgcggag
     1801 gacgagcttg tcggagaagg agtggttcac gcggaggctg agcgccttgc gcaggcggct
     1861 gtccggaagg cgctcggcca cgcggacggc ccccggctcg aacggcccga ggacgacctc
     1921 gtggtacggc gtcgagagga cgccgtcctg gctgctcagg aagagctcga gcgagccgac
     1981 ggtccgccgg ccgagcgtct gcgagacgcc gacggtgacg tcggtcgtcg tccggtctcc
     2041 gcttccggtg gtgagcggct ggcccgggcc ctggtagccg tggccgtcga tgccgatgag
     2101 gtcgatcgcg tcgtcgaagc ggcgcaccga ggcggagagg gcggtgttgg cctggttcag
     2161 ctcgaggccc cagtcgatcc cgtacgagaa ggagcggtag tcgtattcct tggcggcgcc
     2221 gacggtcagc ccccaggtcg agcccctcca cttcttcttc gctttgaggg tgccgaaggc
     2281 gcgctggtcg ctggcgggga tgctcgcgct gctcagctcg gcctggatgt agcccatgga
     2341 ggccgaggag acctggtcga tgccgacgtc ggccgaaagc gtgaggctct cgctcgcgcg
     2401 ccaggcgagc tggaggaccg gcgagtagac gtggagcttc tcggtgccga cgccgccggt
     2461 gacgggcgag cggtcgccgt cctggtcgta gtagccgaag aggaagtcgg cgtcgaggac
     2521 gccgtcgggc ggcgcctggg cgtacgccgc ggaagccgcg gccgagaggg cggcggcgag
     2581 aaggggcctc gcgagccggc tcagctgcac ccgcagcccc cgcccgactt gccgccggcg
     2641 ccgccgacgg cgccctcgcg gtaggtctcg gccgcgcgct cgaagcgatg cgcgcgtccg
     2701 tcgtcgagct tcatcggttc ggtcgagagc gccccgcgct cccacggctg cacggtcgtg
     2761 caccccggaa ggagcgccga gaggccggtc agggcgagga tcaccgggag tcgtctcttc
     2821 aacggtcgcc gccttccttc gtcagcatcg tctcgaggcc tttcgagaag tggatctttc
     2881 cgttcgcgtc gacgagcatc gcctcgacgc cgttcagccg gttcacgagc gcgagcccct
     2941 tctcgacgcc gagcaccgag acgccggtgg cgagtgcgtc ggcgacctcc ccgtcggggc
     3001 agacgatcgt cacgctgcgc acctcggtca cgggccagcc ggtgcggggg tcgaggatgt
     3061 gcgagtagcg ccggccgccg agctcgatgt agttctcgta gtcgcccgag gtgacgacgg
     3121 cctgctcggt cgtctccagc caggcgaacg tccggctctt ctcgaggggg ttcgcgatgc
     3181 cgatgcgcca cggccgcccg ctctcctgcg tcccgaagac gacgaggtcg ccccccgcgt
     3241 tcacgacgcc gcccgtgacg ccgtgctcct tcaggacgaa gacggcgcgg ttcgcggcgt
     3301 agcccttccc gataccgccg aagccgatcc gggtcccctt ccggtcgagg aagacggtgg
     3361 acttcgcctc gtcgaggatc acgtgccggt gcccggcgtc ggcgagggcg gcgcggatcg
     3421 cctcctcgct cggcaccggc gcgttgtgcg acttgaagtt ccagaggcgt ccgacggtgt
     3481 ggaacgtgac gtcgaacgcc ccgtcggtga gcgtcgagac cttcagcgag cggcggacga
     3541 ggtggaggag ctccttcggg acctcgacgg gagcgatccc cgcctgccgg ttgatctcgc
     3601 tcaccttcga ggagtcgcgc cactccgaga ggaactcttc gatccgttcg atctcggcgt
     3661 acgccgcgtc gatcgccgcc ctccccttct ccgggtcggc gtggaccgcc gtgatctcga
     3721 acttcgagcc catcgcgccg cgcgggtcct tcagcgtggc cgctgcgacc ggaccgcgca
     3781 ccgcgagcga caggaggaga accgctccgc cgatgcaccc cgctcctgtt cgcctcgtcg
     3841 cgcggaccat ttcggcggcg gatgttatgc gcggcgccga gcggaagccg aacgacggcc
     3901 gaactccggt ccggttcccc ggggagcccg actcggcccc ggtgcactac atcgccatgg
     3961 agctcgtcag cggtcggacg ctcgcgagcc tgatccacga gaaacggacg gacctgcgca
     4021 cgctcgccgg ctatctcgcc caggcggcgg acggactcgc caaggcgcac gccgccggga
     4081 tcgtccaccg cgacttcaag cccgcgaacg tgatggtctc gacggacggc ttcacgaagg
     4141 tcctcgactt cggcctcgcg aagctgaccg agacgccgag ccgggcgccc ggcctctccg
     4201 acgcgccgac gatggaatcg ccgggcagcg cggcggggag cgtcctgggg accgcgggct
     4261 acatgtcgcc ggagcaggtg gccggccggc cggtggacca ccgctcggac gtcttctcct
     4321 tcggctgcgt cctctacgag gcggcgacgg gccggcgccc gttcgaagcg cctacggcgg
     4381 tggaggcgat gcaccggatc ctgaacgaga agccggtgcc ggtcgaggag ctgaatcccc
     4441 gcgtgcccgc ggagctccgg cgcatcatcc gccgctgcct cgcgaagggg cccgaccagc
     4501 gggtgcagtc gatgaaggac ctggccctcc agctccgcga gatcgtggag gagtgggact
     4561 cgctcccggc ttctggcagc tccgcgacct cctcggcagg cgtcgccgcg ccggggcgga
     4621 aggcgaggcc cctcgtcttc gtggccgcgg gagcagggtt cctggtcgtc ggcgcgctgc
     4681 tcgcgctcgg gctccggggt cgacagggtg cggagcccga gggagctcag gccttccaga
     4741 cgatgaggat gtcgacgcag acgagccggg gcgacgtctt cgacgccgcc ctctcgcgag
     4801 acgggcggta cctcgcctac ctccacggcc gcgccgggct cacgggcctg cgcgtgcggc
     4861 aggtggcgac cgggagcgac gtcgaggtgg tgcccccgag cgaggccggc ctctcgaggg
     4921 tcgccttctc gccggacggg aactacgtct actacctcgc acggaggccc gaggacaggc
     4981 gataccaggc gctcttcgtc gtcccctcgc tcggagggcc gccacgcgaa cggctcttcg
     5041 acgtcgactc gaaggtgacc ttctcgcccg acggaaagcg cctcgcctgc tggcgcggcg
     5101 tgccccagaa gcaggagaac gtcctcctct ccttcgacct cgagagctcg cgggagacgg
     5161 tcctggccac gctcggcgcg gacgagccca tggtgaccgc gcccgccggc ccgccctgga
     5221 gctcccggac acgttcctct cgagcgtcgc ctggctcgcc gacggcagcg gcctcgtcgg
     5281 gacgggcatc agcctgaagg ggatcttcca ggggcagacc ttcctgatcg gctatccctc
     5341 gggccggctt cagcgcgtca cgaacgactt ccacgactac gcctccgtct cggtctcggc
     5401 gacggacgag gcgatcgccg cggtgcgcac gtctcgcctt gcgaacctct ggatcgtcga
     5461 tacgtcggac gccgccgccc ggaagctcac ctcggtcacg agcccggagg gctcgccctg
     5521 gaacatggac gtcgccggtg cggaccaggt cgtctacgag gtgccgctcg acggcacgct
     5581 ccggatctgg agcgtcgatg ccgcgggggg cgagccgcgg tctctgacga ccgactccgc
     5641 gcacacgttc aacacgaagg ccgccgccgg ggtcgtcctc ttcgaccggc tcgacgacac
     5701 gggcgtgcac atctggcgga tggcggccga cggcaccggc cgcaggcagg tcaccacggg
     5761 aagcggcgag caggtccgcc acctctcgcc cgacggccgt ttcgtcgcat tcgagcgatg
     5821 ggacgacgcg gggagcatcc acctcttcga cctcgagagc ggaaaggcgt cgaagctgtt
     5881 cgagcgcgcc gccgacgtct tcgggttctc tccggactcg aagcggttcc tcgtcacccg
     5941 gctcgagccg gacgagaagg ggcagccacg accggtctgg caggccctct ccgtcgaggg
     6001 gaacgaggcg cccgcgacgt tcccgggtcc ggcgggcgcc ctgggcgcgg tctggacgcc
     6061 ggacagccgc ggcgtgagct tccgcaggcg caacgacccc gcctggaacg tcttccgcca
     6121 gggactcggc gagggcgagc cggtccaggt gacgcgcttc accgaaggcc ggctgatggg
     6181 ccacttctgg tcgcccgacg gccgcaagct cgccgtcacg ctccggacgc gggagggggt
     6241 cgacctctgg gtcacggagg ccgacggaac ccgccccgtc cgcgtcacga ggctcccgga
     6301 gctcgaagtt ttcggcgtcc gctggctgcc cgacagcagc cgcctcgtcg tatcggccgg
     6361 gacccggagc caggacgccg tcctgattcg cggctttcgc taggcggccg gagcgcttcc
     6421 ccgttcgcgg ccgagccgcg agagcgggcc gaccgcgtag ctgcaggcgt gctctccctc
     6481 cgggatgaac gtctcccttc gcgacctgac gccgagcagg ccctcgatca gcctcttctc
     6541 gaaccggcac ggcaggtcgg agagcgcgac catctcccgg agcgggcagt ggcagaggcg
     6601 caggcgcggg ccgccgtcgc ccgcgacgac ctcggcgacg aaaccctcct cggagaggat
     6661 tcccgcgatc gcctcgagcc gttcgcggcc gcgcaggccg gccacgcgcg gcgccagctc
     6721 gcggcgccgg cgcgcgaggc ggcgctcgaa gaactcctcg aggaggtccc ggcgcccctt
     6781 ctcgtggagg aagctcgcga gctccccgag aagctctccc tcgcggcgcg ggaagagcgc
     6841 ctccccggcg gccgtgaggc ggtagaggac gtgaggccgt cccggtccct tccggtggac
     6901 gcccggccgc tcgacgaggc ccatcgcgac gagcgacttc aggtgggtgc gaagcgtctc
     6961 gcggttcagg tcgaagccgg cctcgagccg cgcgagcgtg cactcgccgg accgcttgat
     7021 gaggccgagg agcctccgct ggctctcgcc gatcccggga ccgaagaccg attccattcg
     7081 ctgcatccgc tcgtctcgct cagacccgcg ccgcgcccgg ggcgacgccg acccgccccc
     7141 cctgcggccg gctcgccgcg aggaggcggg gaacgatcca ggtcgcccac cagacgaggc
     7201 cggagacctc gaggaggccc gagactccgg ccgtcgggaa ggccaccggg gtgaggtcgg
     7261 tcagcacctg ctgcgtcacg cgcagcgtgc agccgaggtt caggaggacg aacggcgcca
     7321 gcggcatcgc ctcggcgtcc gccgagcttc ccggcggacg gggcgcgacg cgcgaggcca
     7381 ctccgacgat catcaggctg acgaagccga ccgtgatcgc gtgccgggtg gcgccgtgcc
     7441 acgcgtgcga gaacgggatg ccgacgaggc gcgaccagaa cggcccggcc acgagcatcg
     7501 cgagcgagac gccgagccag acgtgcgcgg cgcgcacgaa ggcaagcggc gagaggcgcg
     7561 gcacgacgaa acgcggtcgg gcgaaggccc ggaggctcga gaccgcgagg gccgcgcaga
     7621 cggccaggcc gacccaggcc agccagacgg cgacgaagag ggccggccgg cgggaggtca
     7681 tcgccaacgg gaagagggcc acctcgagga cgatcatggc ctggagcggt ccgaacaggc
     7741 ggacggcgcg gcgctcgtcg acgaccggag tcccgaagag gccgggcagg actcgcatcg
     7801 agacgccgag gatcatcgag agcgcgagac cgtggatctg caggtcgcgg agcgggagct
     7861 gccacaccgc gatctcggcc aggagcgacg cgcggtcggg cgcgccgatc gtctggacga
     7921 gatggcgcag gtccaggacg gtcgagagga gaaaccagac gaggccggcc ccgatgtaga
     7981 ggtccgccgc ccggcgtcgg acccccttct ggatcgccgt cgccgccagc agggcgacga
     8041 aggccgcgac gacggcgagc tgcagcgccc cggccgcgac cgcgagcgtg gcgaaggggc
     8101 cgacggggcg cgcctcgccg accgtcctcg cgacgaccgc gagagccatg agcggcagga
     8161 gggcgcgggc caggcgcggc gaggagagcc gcacgcccca gaacgcgggg aacatctgga
     8221 gcgcgaaccc catgatgaag agccccatcc acccgtagat ctgcgcctga ccgtgcgcgt
     8281 tgatctcgaa gatcgagggc gccgagaagt cgtgggcccg ggcgatgcgc aggaggagcc
     8341 acgcgcccca cccggctccc gcggtcagcg tgacggcgag gccggcgagg aagaagcgcg
     8401 ggtagagcgg cacccgcgcc agggcgggga gcagggccgg ggaagccccg gccgcgagcg
     8461 ggcagacgga cgacgcggac ggcggaccgg cgggtgcgga cgaggcggcg gggatggggt
     8521 tgcttgccac tgttatcatg atgttatggg tgacaatcag acgcgtcaat cccgcgaggc
     8581 cgacggttcg cggccgccgg ctcccccgcc gtcccggctc gactaccacg aggtcgcccc
     8641 cgacggcttc gccgccatgc tcggcctcga gcgctacgtc cgcggctcgg ggctcccgca
     8701 cgccctgctc gagctcgtga aggtccgcgc gagccagatc aacggctgcg cctactgcct
     8761 cgacatgcac acgaaggacg cgcgggccgc gggagagacg gagcagcgcc tgtatgccgt
     8821 cgcggcctgg cacgaggcgc cgttcttcac cgaacgcgag agggcggcgc tcgcgtggac
     8881 cgaggccgtg acccgggtcg gcgagagccg ggtgccggac gagctctacg aggaggtgcg
     8941 ccggcacttc gacgggaagg agctcgtcga cctcaccctg gcgatcgtcg tcatcaacgg
     9001 ctggaaccgg ctcgcggtct cgttccggac ggccgccggc agctaccggc cggacctggc
     9061 gcggtagggc gggggcgcgg atgtcgccgg acggcgggtc gccgcatccc tccgagaggc
     9121 cgctctcccc ctggtggcgt cacgccgtca tcctcgtgat gacggtgggg ttcagcctcc
     9181 tcacgctcgt caccgtcctc acgtacacga acgccccgcc cgtcccggag cgggtgaccg
     9241 acgcggccgg cgcgagcctc ttcacgcgcg agaacgtcga gcacggccag gaggtcttcc
     9301 tcaagtacgg gctcatggag cacggctcgc tctgggggca cggcgcgtac ctcgggccgg
     9361 actacagcgc ggagtacctg caccggctcg ccgagatcgc gcgggagacc ctcgccctcg
     9421 agcgtcacgg caagccctac acggcgctcg ccccggacgc ggccgcggcc gtcgccgcgg
     9481 gcgtctccgg gcttctcaag gagaaccgct acgacgaggc gagcgggacg ctccgcttct
     9541 cgccggccga ggcgtcggcc tggaggatcc agagcgcgga atgggccgcc tatttctcgg
     9601 gccccacgcc cgcgccgggc ctgcccgcgc agtacatccg cgaccctcgg gacctcgccg
     9661 acctcaattc gtacttcgcg tgggccgcgt gggccaccgt cgcgaaccgg cccggcaagg
     9721 actactccta cacgaacaac tggccctacg agccgctcgt cgggaaccgg ccgagcgcct
     9781 cgacctacct ctggagcgcg ctcagcctcg tcaccctcct cggcgggctc ggcgccctcc
     9841 tcttcgcgtt cggccggttc gacttcctcg gctggcacgg cccctcgggc gccggccacg
     9901 agcacgagag cctcctcgcc cgctggaccc tgacgccgag ccagaaggcg gtcggcctct
     9961 acttcggggt cgtcgccctg ctcttcctcc tccaggcgct cgccgggggc gccctcgccc
    10021 actaccgcgt cgagtcgggc gcgttctacg gattcgacct cgcccgcttc ctcccctaca
    10081 acctcctccg gacctaccac ctccagctcg ccatcttctg gatcgcgacc gcctgggtcg
    10141 cgggcgggct cttcctcgcg cccctcgtcg gaggcgcgga gccccggggc cagcgcgccg
    10201 gcgtgatcgc cctcctcgcc gctctcgccg tggtcgtctt cgggagcctc gggggcgaat
    10261 ggctcggcat caacgaccgc ctcgggagcc tctggttctg gctcggccac caggggtcgg
    10321 agtacctcga cctcggccgg ttctggcagc tcctcctcgc cgccggcctc gcgggctggc
    10381 tcgttctcat gtaccgggcc cttcgcccgg cgctccgcga cccgaagcgc tcggagctcg
    10441 cctcgctctt cctctacgcc gcggcggcga tccccctctt ctacctcccc gcgctcttct
    10501 tcggcccgcg cacgaacttc gcggtcatcg acaactggcg gttctggatc atccacctct
    10561 gggtggaggg cttcttcgag ctcttcgcca cggtcctcgt cgccgtcatg ttcctcgtcc
    10621 tggggctcgt cacgccccgc acggccacgc gggtcgtcta cctcgacgcc atcctctacc
    10681 tcgccggcgg gatcgtcggc accgggcacc actggtactt caccggccag gggacgctca
    10741 acatgggcct cgcggcctgc ttctcggcgc tcgaggtcgt gccgctgacg ctcctgacgc
    10801 tcgacgcgtg ggacttcgtg aagctccgcg agcgacgctg cgacgactgc cacgccacct
    10861 tcgccgaccg gcaccggtgg accgtctact tcctgatggc cgtcgggttc tggaacttcg
    10921 ttggcgccgg cgtcttcggc ttcctcatca acctccccgt cgtctcctac ttcgaggtcg
    10981 gcacgtcgct cacgagcaac cacgggcacg cggcgctctt cggcgtcttc ggcatgctcg
    11041 ctctcgccgt cctcgtcttc acgctccgct cgctggcgcg cgacgaggtc tgggcgcgcg
    11101 cggaacggtt cgtgagggtc ggcttctggg gtctcaacgc ggggctcgcg tcgatgatgg
    11161 cggtcgacct cttccccgcc ggcgtgctgc agctccagga cgtcctccag aacggctact
    11221 ggcacgcacg gcggctcact tacctcatgg agggcacctt ccacaccctc gagtgggccc
    11281 gcgcggccgc cgacggcctc ttcctcgtcg cgggcgtcgt gcccctcgtg ctggccgtgg
    11341 cggtcctgct cctcggctcg cgcggagggc acgcggcgcc cgcgggcccc ggcggcggta
    11401 cgatttccgg atgaagacga cagcgggact cctctccctc gctctcctcc tgaccggctg
    11461 ccagaagacc gaggcccccg cgggcgagac gccgaaggcc gccgccgacg cgcaggcggc
    11521 cgtcgcgcag gcgcccgcgg tcccggcgag cccgtccgac ccgaaggcgc tcctgaccga
    11581 cgagatgctc gggaagtacg tcgtctacca gaccgcggtg ctccccgtca tgggcgatac
    11641 cctggcgatg ggcggcagcg ctcttcaggg ctccggcggc gagcagaagg agttcgagaa
    11701 gcaggtggcg gccgacccga ggctccagcg cgtcgaggcg gccacgaagg aggcctccgc
    11761 gaagtccggc ctgacgccgc agaccgccgc cgcgatcggg ggcgtcgtct cggaatacgt
    11821 ctcggcgcgc tcgatggggt cccccgagga gaaggccaag gtgagggccg acttcgagct
    11881 gaagtacggc acgcccgcgg ctgaggcgat ggaccggcac gaggccgagc ttacgaagct
    11941 ccaggaggag atgctgaagg ccgccctcgg cccggcgaag aagtagcgcc gccccccccg
    12001 ggaggccgtg caccggccgg aagaaagacg cggcctcatc gtctatcgtc cgcgctcgga
    12061 ggcgcccatg acacgcgtga ccggaatcgg cgggatcttc ttcaaggcgc gcgacccgga
    12121 gtcgctccgc gagtggtaca ggaaacatct cggcgtgccg ctcgaggaat ggggcggcgc
    12181 ggtcttccac tggcagacgg cggagcggcc ggagccggac gggatgacgg tctggaacat
    12241 cttcccggcg acgacgacgt acttcgcccc gagcccggcg cccttcatgg tgaacttccg
    12301 cgtggaagac ctcgacgccg tcctcgcggc gctccgcgag gaagggtgcg cggtggacga
    12361 gaaggtggag aggtcggagt tcggagcctt cggctgggtg atggacccgg aggggaaccg
    12421 cgtggagctc tggcagccgc cgaagagctg agggggagcc ggaccgaatt cggggaattc
    12481 tctgggatcc tggcccgggg gttgccgagg agagctccac ggaccacctt cggaacagaa
    12541 ggtgtccggc tcggtcgtcg agggtcgcct gacctccggg aggcccgatg acgaagggtc
    12601 cgttcgcagg gaggtgatgc cgggagcgaa cggtgcggca ggtcgagtgt tccgaaaccg
    12661 tccacgactc cggcgcggcg ccgcgggcgc tggcgatggg tctgctcgtc cccattcccg
    12721 acagattcac aacgtgcaac cgggagacat gaagatgcga tccctccttt gcagggtctt
    12781 cccgctcgtg ctgctcgcgg cgttcgtcgc tgccccggtg acggcggacg acgcggcgct
    12841 gttccggatc ggccacgtcg tcgtcgtcgg cgacgtccgg ctccccgcgg gggactacac
    12901 gttccgcgcc acgcctcgcg gcctcgtcct cgtctacgac agggagatga agaaggtgca
    12961 ggccgcggtc ctcgtggaac gctcgccgct cacctgggcc gaacacgaga aggcgaacac
    13021 tctgtcgcac gactgggccg tccgctcgct gtacctcggc gagcacgggt acctcttcgc
    13081 gcccggcccg gtcccgccgg acctggtcgc gaggtgcgga ccggcgacga cggtcgtcgc
    13141 gcaggtccgg tagagacgcg ggtccgcccg cctacgccgc cgcgagggtg acgagggcgg
    13201 cggcgaggcg gacggcgtcg cgcaggaggg cgagggtcga ggcgaggtcg cgaagcgtct
    13261 cgagggaacg cttcagctcg cggttcttcg ccttcagctc gtcgtggatg gcggcgacga
    13321 cgccgctctg gtcttcgagg caggcgagga cggccttcca gtaggcgtcg cgcgcggcgc
    13381 catagagctc ccgcacgcgc tccttctcct cggggccctt cgcagcggag tggagtgcgt
    13441 cgaagctccg ctcggcgagg cggtaggtcg cctcgacggt cgcaaggagc cgttcgtggt
    13501 tcgacttcgt ccgggccatg gccgcctccc ctacttcccc gccgccgcgc ggtactcgtc
    13561 gaggagcgcg cggacggccg cgacgtgccc gtgtacggcg gcgaggtcgg tctcctgctt
    13621 cgcctcgaag gaacggtgcg cgaggacgag cgcctcgagg ccggcgagcg tctcgtcgac
    13681 gagcgcgccc tcgaggtcga ccctccgcgc gacgcggtgc tcgaggacgc ggtcgagcgc
    13741 cgcgcgcagg tcgtcctccc ggttcgcgcc gggccggccg gtgatcgacg gctccttccg
    13801 aaggagcgcg agggcgccgt cgtactcctt ctcgtcccag gcgaggccga aggccgcggc
    13861 gtgcggggcg agaagcgtgc ccggccgggc gagcccgagg gcggcgaagg cgcgcgccgc
    13921 ggtcccctgt ccctcgacga gcgcccgccg gagggacgcg tggacctccc tctccgttcg
    13981 gaggagggcg acgaaacgct cgaggctctc gcggatcgcg gcgctcgcct tcttcaggcg
    14041 cgcggcgtgc cgggcgcccg cgaacgcgcc gaggcccttc gagaggccga aggccggaac
    14101 gggagcggcg gcgggccggc cgagcgcctc gcggtaggcg ctcacggcgc ccgcgaggtc
    14161 gttcacgccc cgctccacct gatccgcggc gtcgaacgcc gcgagcgcgt cgagcccggc
    14221 gtaagccgct cccagctcgg acatcgcgtc cccgcgtgcc gcgaggacgg agcggacctt
    14281 ccgaagggtg gcgagcgtct cttcggccgg gtcgggacgg ccggtgagcg gcgcgaggag
    14341 gagctgcgcc tcgacgtagc ggtcgacgga ggctgcggcc tcgcggaccg agtcggccgc
    14401 gacgcgggtc gcggcgcctc ccgcggaagc cagagagccg gcgcgcggcc gcgccgtcga
    14461 cgtgcagccg gcgagcgccg cgagagacag gaggacgacg gagacgcgga ccggtcggtt
    14521 cacgaagcca cccccatgcc gcgaagtccg gggacgaaag gctgcagaag gttaccgcgt
    14581 gcgcggtcag ctcttgccgg aggcgtcctt cttctcgggg gcggggccgg acgacgccgg
    14641 cgccggcgag gccggaacgc tcttcccgcc catgtcgacg ctccccttga actgcgcccc
    14701 ttccgcgatc gcgattttcg gggaacggat gttcccctcg aggatcccgg acgggaggat
    14761 ctccaccttc tcggcggcga cgacgttccc gacgacgcgg ccggcgatga cgacgtggtg
    14821 cgccttgacc tcggcgttca cctgggcctg cgggccgatg cggaggccct tggagacgga
    14881 gatcttcccc tcgacgcgac cttcgacgac gaggtcttcg tcgccggaaa gctctccctg
    14941 gatgcggatg ttgggtccga tcacggctgt cttcacctcg gttgcggcct tcgggcccgt
    15001 cggggcgggc gcggccgcag ggcgcgggga atcgtgagac ggtgcggggc gctcgccttc
    15061 ggtgcgaggc gccggcttcg atccgaaaaa gcccatcggc tcggaagtct acccgatccg
    15121 ttcgggaggc ccggagggcg ggtccgccaa cgccgcgagg acgaagccgt cctccgcgcg
    15181 ccaccagccg ctccactcgg aagggcccgg tccgcgcgcg ccgaatcgtc gccaggagcc
    15241 gtcggccgga cccggagccg gtgcggcctc gacgtcccgc ggggcgatcc ggagcccctc
    15301 gcggagggcc ttcatcacgg cctccttcga gcaccagacg agcgtcgcgc ggagcgcggc
    15361 ctcccccgcc ggggctccct cgacgaaccc ccgctcggaa ggtgtgaacc agtcctcgag
    15421 gaaggcgccg cctcgtacct cgattcgctc gaggtcgagg cccagccgcg ctccggcggg
    15481 ggcgatcgcc gcggcggcgt ggccgtggct gtgggtgatc gagaggacga acggggcggg
    15541 gcggccgccg acgtgcgcct cgggcgcccc ggagtccgcc gcgaggacgg ccgcctcctc
    15601 ctcgcgggcg atggcgcccg cgaggaggag gaccttcttg gcggcgaaac gcccgaggag
    15661 ccagtctccg cgcctcttcg ggacggtgag gcgcgagagc gcgaggcgct cggcgggcgc
    15721 gagccacgcc tcgccgcgcg ggaggtcctc cgaccgcgcg gccgcccacc ggacggcggt
    15781 cagcgggcga tccgttccat ctccttcgcc atgccgcgct cgatggcgtc gacgaggtag
    15841 gggcggagct ccttcgccgg gaggatccgg tcgagcgagc cgaccgcgag ggcccgctgg
    15901 acggaatggg tccggtcgaa ctcgtcggcc acctcgccca gcttctcgga gcggacggcg
    15961 gcgacgaggt cggcgtgccg ggagcgaagc ctgcgccgct cgacgtcgga aacggcggcc
    16021 atctccttct cgagcgccgc gacgcgcggg tccttcttcg tccgctggtc gacctcgcgc
    16081 gcgaagacga cggccgccgc gggcgcgccg ccgatgacgg aggcgtaggt cccctcgagc
    16141 gccgcgacct ccatgttgtc gttcagcgtc ttcgagaaga cgacgaacgc gccgccgtgg
    16201 tagcgcgaga cgacggtgaa gacgatcggt cccctgaagt tcgtcacggc ccgcccgatc
    16261 tcggcgccgt actcgagctg gatcttgcgc atcgactcgg gcgagccgtc gaagccggag
    16321 aggttcgcga ggacgacgag gggccggttg ccgctcgcgg cgttgatcgc gcgggcgatc
    16381 ttcttggacg cctgcgggaa gagggtcccc gaggtccagt tctccggacc gtccgtggcg
    16441 acgaagccga tccgcttgac ggggtgcgac tcgaagccga ggaggcagac gggccagccc
    16501 ccgatgtggg cgtcccagac gacgccgttc tcgccgtcgc gcaggcccgc ccagcgctcg
    16561 agcggcgcgt ggtcctggtc ggccgcggcc gccatcacct tgcggatctc gaaggggcgc
    16621 ttgcggccgg ggttcgtggc gtcggagaag atctcgccca ccagggcgaa cgcgcccggc
    16681 tcctcgccgc cgtgcggcga gaggcagacg tcgcggccga tcgggtccga cgtctcggcg
    16741 cggcgcggga agcgctcccc cgggacgacg taggtgtggt cgtagtggcg gagcaggatg
    16801 cggcaggcgt cggggatgtc gcgcgcccag tactgcgcct ggccgttcgg ccccatgacg
    16861 cgctcgtagc cgccgatgcc gaggttgtcc tcggccgaga ccgagccgga gtagtcgagc
    16921 gcggtcttcc cggtgaggac catcgcgccg tccttcgtca tgacgaggat gccgcgcgtg
    16981 tgcatgagca tcgtcgcctc ggcgttccag tacggctggg cgccgacgtt gatgccgcag
    17041 acgacgacgt tcacctcgcc cccggcctgc gtgaacgtga tgaggcggcg gaggacgcgg
    17101 gagatccagt ccatgttctc ggtgcccgag tccatcgcga tcttcgcgcc ggcgaagagg
    17161 gcgtaccact cgacggggac ccccatcctc tcggcgaggt cgaggccggc gaggatgcgg
    17221 cggcactcgg gctcggcgag ggagcccatc gcccccatcg ggtcgc
//
LOCUS       DAOTEZ010000031        17198 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2253_length_17198_cov_6.841626, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000031 DAOTEZ010000000
VERSION     DAOTEZ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17198
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2253_length_17198_cov_6.841626"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="P5164_02790"
     CDS             <1..480
                     /locus_tag="P5164_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42851.1"
                     /translation="APPAAGDGRAWAAVVLSGSAAGDLFFAAEGALALPAGVAALLDG
                     AGTADEGAPRRIAREALTGSAVPGVTGARLLCTSEARSVLRLGPPPAARWAVEGLRSV
                     AVLAGKLTLVDGEEARDVRAGEVAFVAEPAATLHVQAGNDAAVAIGFGRPELSVRLG"
     gene            complement(486..1019)
                     /locus_tag="P5164_02795"
     CDS             complement(486..1019)
                     /locus_tag="P5164_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRY42852.1"
                     /translation="MYARPLFCSQCGQRLEERSVGAGDGRTVPVCPACGTVHWIDPKV
                     AAGCLVVRDGRVLLLRRGIEPGYGRWVFPGGHVDRGETLEEAALRETREECGALARID
                     GLLGVYSYPGRPVIVAAFRASLLPGSPEPYAADETLEVGWFAPEEIDRLDLAFRSSAD
                     ALSALLGRLVRAPGRDG"
     gene            1187..1855
                     /locus_tag="P5164_02800"
     CDS             1187..1855
                     /locus_tag="P5164_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42853.1"
                     /translation="MSRHWTQDEIRWLNANVERMDLPSVSRALGVPLVEVERKLAELR
                     ADAAAAQRKPPATHREAARELTHARKLFEKGMEHLHRREMPAAAACFSELLELRPDER
                     ELVDRARTFLAVARNGKSVKEKPLRDPAEIYHAAVFEKNRGNCATAIELVRSADGQAD
                     PDGRLAYLAACCYALHGRPEEALESLRAAIAASDQNRIQARLEADLAPLRSQPRFESL
                     VGRA"
     gene            1858..3276
                     /gene="glmU"
                     /locus_tag="P5164_02805"
     CDS             1858..3276
                     /gene="glmU"
                     /locus_tag="P5164_02805"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HRY42854.1"
                     /translation="MPAATAVVLLAAGRGVRMKSARPKVLHEAAGRALVDHVVAAART
                     FLAGRPGSRLVVVAGAGRDELVPHLARTAPEALVAVQDPPRGTGDAVRVALSALGDAA
                     RVVVLAGDVPLLTAASLARLDEALDAGAGIAVLTALLPDAGAYGRVVRDAAGRFERIV
                     EAKDASPEVRALGEINSGIYAFDRELLERELPRLTDANAQKEYYLTDVLGFAVGDGAA
                     VAALPVDDPAEVLGVNSRLELAEVDALLRLRAARAAMAAGATLIRPETITLDEGVTFG
                     SDVVVEPFVSVTGASSVGAGTRIGQGCVIHASTIGANVSVRPYCVLEKAAVGDGAVVG
                     PFARLREGTDLGEGVHVGNFVETKKARLEKGAKANHLTYLGDCRIGERTNVGAGVITC
                     NYDGYGKHFTDVGAGVFIGSDVQLVAPVAVGDGALVGAGSTITKDVPADALATSRVPQ
                     KTIEGGGARYRERKTAAKAAGK"
     gene            3490..5334
                     /gene="glmS"
                     /locus_tag="P5164_02810"
     CDS             3490..5334
                     /gene="glmS"
                     /locus_tag="P5164_02810"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRY42855.1"
                     /translation="MCGIVGYVGPREATPILVDGLRRLEYRGYDSAGIAVLGADGLVR
                     VTRSEGKLGNLTARIEAEPPSGRTGIGHTRWATHGRPSEENAHPHRDASGRVVVIHNG
                     IIENFLPLKRELLARGVPFTSETDSEVIAQAIGAACADAPEAPFGEVVRTVLSRMTGM
                     YAVVVLSADEPGVLYVAKNGPPIVLGLGADENLVASDATALLSHTRDLVFLEDGDFVR
                     LTAASVAVTALDGAPRSRPARRVPWDAVAAEKGGYPHFMAKEIAEQPTVVVETVGHKL
                     SLETGHFSEDAMGVSPKLLADVDRVVVVACGTSWHAGLVGKFLLEEVARVPVEVDYSS
                     EFRYRKNLVGPRTLVVGISQSGETADTVAALTDAKRAGAPTVGVVNVPGSAIARLVDG
                     VLATHAGPEIGVASTKAFTTQLVALALLAYYAREARGLGRLEPGDPFLEGLAHLPVAL
                     QQALALEPAIEKLSRALAHARDVLYLGRGAHYPVALEGALKLKEISYIHAEGYPAGEM
                     KHGPIALIDGELPVVALAPRDALYDKVASNLREVKAREGRLVALVSPGDEDVSTFADE
                     TLVVPEIHPALQPVVSVVPLQLLAYHVARRRGCDVDQPRNLAKSVTVE"
     gene            5363..7666
                     /locus_tag="P5164_02815"
     CDS             5363..7666
                     /locus_tag="P5164_02815"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRY42856.1"
                     /translation="MLNVREKALLEGLNPEQAAAVSAGPGPLLVLAGAGSGKTRVLTR
                     RMAWLMAHGTPEPSIVALTFTNKAAGEMKERVAHLLGSRTPRSFVGTFHAYGVRLLRR
                     FASEAGLTPGFVVFDADDQLAVFRQALKAAGISDKALTPKAVASKISRARNAGWSRDE
                     YPRRFGDFVGTRLAELHATYEKELKKANAVDFDDLLVRTRGLLERSPEVLALLRRSVT
                     QLLVDEYQDTNGAQAGIVRLLAGEARNVFAVGDEDQSIYRWRGADVSNILDFERDFPG
                     AETIRLERNYRSTAPILDAAGAVVAENRRRLGKSLKATKPGGEPVTLLVLPEERDEAR
                     EVVERISRLKAVKPGAEVAVLFRTNAQSRPFEDELVRNRMPYLLVGGTRFYERAEVKD
                     ALAYLRLLRNPEDDVSFRRVVNVPARGIGAVTLEGLSAAAAECGSSLFAVLSALPDGL
                     TERARKALVEFRDLVSSLREEFAAEDAGAAAAVAAVLSNTGLLALYEGSDDPQDQARR
                     ENLDELLAAAREHERSGAEGEEADRSLAAFLDAVTLRSDADDVDERKGIVLMTLHAAK
                     GLEFDEVFLAGMEDGSLPHFSSRQDEDELEEERRLAYVGMTRAKEKLTLSRVLRRMVH
                     GEWTSREPSPFLDAIPERALAVVDRTFGPGSADGFRGGGRGAGLFPDYEGESQETWAP
                     PRSAARPGYRPAARAAKPAIPTMRRTPPPPTASGFRRGGRVRHPEYGVGVVLLVEGSG
                     DGEKLTVYFDRAGRKKFVARFSNLVPA"
     gene            complement(7707..8216)
                     /locus_tag="P5164_02820"
     CDS             complement(7707..8216)
                     /locus_tag="P5164_02820"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRY42857.1"
                     /translation="MSLPHVLLGLLSRAPASGWDLKSRMARDLALGWDAELPQIYPAL
                     RRLLRGGFVVMKRRRSEKGPPRREYRITPAGRRELREWLAEPLAMPRPRDAALARIAF
                     LERQPREARAARLKEYRALLVAALKKASPGSTAARRRWRALLEAERAWADAERALLEA
                     AVEEPAPPR"
     gene            8832..9695
                     /locus_tag="P5164_02825"
     CDS             8832..9695
                     /locus_tag="P5164_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010041833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY42858.1"
                     /translation="MSNVDASPLVLAHSPDSDDAFMFYGLRAEAVDTEGLVFRHELAD
                     IETLNGRALRGELAITAVSFHAYAHLAKTYVLLPHGASFGDGYGPCVIAREAGDAPAA
                     LEALAGKRVAIPGALTTAALALRMRVPSALPVVVPFDRVGDAVAAREVEAGVVIHEGQ
                     LTWKEEGFRLLGDLGALWTKETGLPLPLGGNAVRRDLGAEKIGRISRALGRSIEYALA
                     NREAALTDAMRFARGLPREKADRFVGMYVNDWTRSYGERGREAVRLLLDRAFEAGVVP
                     TRVEPEWVEER"
     gene            9692..10240
                     /locus_tag="P5164_02830"
     CDS             9692..10240
                     /locus_tag="P5164_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42859.1"
                     /translation="MSLERFTRGSALRTALLLVLGALLGALVFFAGAAGTVLRESPSR
                     HAGGAVNRALLDLLDLSTYGVAAVAFVLVLVLDRVEPFGKVQKGLALRLLAVLAAAAF
                     ASHEVITPQMMSLRDRLPTLIDLLPAGDPGRKAWGRLHGFSSGILVVRIATAAFLFLL
                     LARKRRRTIATAEVPAGVPPAA"
     gene            complement(10262..11569)
                     /locus_tag="P5164_02835"
     CDS             complement(10262..11569)
                     /locus_tag="P5164_02835"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRY42860.1"
                     /translation="MRRSVLLAAFALAVVASPAAPSRAQGISFSEGRPFAEALRRARA
                     EKKPVLLDAYAVWCGPCKQLDRLTFADPKVGEWARKNVVAVKLDAEKGEGRRISQRFA
                     VRAFPTVLFIDPAGNEIDRLSGVFGPQEFLGAADAILSGKTPLAAAIAKLKETWDPAL
                     AGGLAAQLAQRNDLARLRPIALRAVEEDPSLAEEGAREAFLYLVSLEHGAGALSAETL
                     DLIESLAPRLAADPRAAILHLAAAEAQARAGNGAAARASALAGLKLLPEDAPLAADLH
                     VALAAAERASGKPEAALAAARRAVALGEKASGGAWARASRLLALAETLAFARQGDEAK
                     KTLEVALGSVSNDPALLVRASALLVRLKDLPAAVARARSAVDASQGGDARAQAALGEA
                     LAASGDVKGAVAAFGRAVELEPENRDARRRLESLRGRTAKKPA"
     gene            complement(11641..12474)
                     /gene="truA"
                     /locus_tag="P5164_02840"
     CDS             complement(11641..12474)
                     /gene="truA"
                     /locus_tag="P5164_02840"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRY42861.1"
                     /translation="MIPSRAPSKTSAPAGAVLRAKIEYDGSRYRGWQAQVNARTVQGV
                     LLAAARELWGPGVRVHGAGRTDAGVHALGQVASVHLPPRARVDAAAALRRWNELLPFD
                     VNVLALAPAAATFHARHDAVRRVYRYRIATRRTAFGKPFVYWVKDDLDVTAMDRAARL
                     LAGRHDFAGFCENPEGHDSTIVVVEEARVAAPAEADGLVLVRIAASHFLWKMVRRVVG
                     VLLEVGAGRMAEAQVARLLAERTSEVARLTVPPSGLFLESVGYPGEPVEEPPHPLRPA
                     F"
     gene            12529..12969
                     /locus_tag="P5164_02845"
     CDS             12529..12969
                     /locus_tag="P5164_02845"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY42862.1"
                     /translation="MRRRRTIHLSVEADFPSVRLLAAACHGVLAEWGLPTERRSLLEL
                     AVVEAATNVVRHAYRGHGGRVEVELTREGRAIALSVVDTGISFDPTRVPPPRDLDPDD
                     PSTWPEGGMGLPIIRAACDELRYSSDGARNELTLRLTVDEPEEP"
     gene            12966..14132
                     /locus_tag="P5164_02850"
     CDS             12966..14132
                     /locus_tag="P5164_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42863.1"
                     /translation="MTGADRPFTAAAVPAVERRCRETLAALLSRLGEVAGDGLAGAAL
                     GGALGRGEAPTTVDANGAFVTPVPFELLVVLDATPGRAASVAPRLRRELAATARTRSA
                     DVRLATAARSALAQLPPSIANVETAAAARVVAGPAALLAPLAPLSTARPGADEALALL
                     VRRGASLLLAERALSRRAPSRAALLAGQAAVHAADLALGTALLVSAGRFRPTDAARDA
                     ELRLLAAPAGDGPAPAGFHLRMSRTRFRDVVERHRAAVEAAREVDLPDSLGEVRAQAG
                     RATDRFVEVLRLLEEERLSRPLPSWTDYVRALAERNGGSPGPGLFEASREEELPSRRS
                     VRAWPVAERLAPALATLLDWDPGDLAVVPLLLDLPADATKEALGARLASLAASA"
     gene            14173..15924
                     /locus_tag="P5164_02855"
     CDS             14173..15924
                     /locus_tag="P5164_02855"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY42864.1"
                     /translation="MTSVTEMADDPGASGRLLFVLALLAGLLLRLVQLGSPDLLGPEE
                     GPWAVGARNIVEGGLAQLLALSSTPLGEPTGIPIFFPALLAAAVKTFGAYEWAIRLPS
                     VLFGLVAAFVLERIVRRGFGQPAGHVAGAFAALASPLVLSSRTATVEPTLTLLGLGGI
                     IFGLRAFEEDSPGEALPAGILFGLGFLTKGYAVGLFVIPILLALLFRPRLFTLGRTKR
                     TLALLAVSFLLTAGSHLLLMALVHPTSLGPHLSRAFGSAPDTLRQLTDVALVSADLRM
                     IVKSLFVLLPLVGVGLAYLARPLGEEEVVSGATGGERRLSHMALWCAYGVELVVLVAV
                     AGSLELSSTPVLPALSALAGLGGAALLTRPRSPRRVRFETIATVASGAAVLVLAILLV
                     WTAREPLFNGGRAAPFTAAGALAAIALSTAGAAWLVSGDAERRFGRRAGLVFLSVLLV
                     SEGLAAARAIRHDLLAHRTGVRALSDQVAPELLPLSPRVVAFRAPEPSAIAFRLFRTG
                     TTWADRPTVEQVAAEARGGKTVVWAWREGTTDPVAAPAPLVRAWLIVNTREVTAEVDA
                     RAGRTTGLRAFVARPPS"
     gene            15936..>17198
                     /locus_tag="P5164_02860"
     CDS             15936..>17198
                     /locus_tag="P5164_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRY42865.1"
                     /translation="MTTVLLADRDGRALGPLEDKTVPALLPLRGAPLLERALEALVSA
                     GVRSALVVVGPRGPEIEKRFGKGIRWGIALEYVRRAEDETTGAVLRRLEHRLDGETLV
                     LRGDAAIEGAFGEFLRRSATSEEPVVAALSGERLLGMWRVRPEALKKLEIPREPADES
                     WVREKGHAPLDVDLDLAPLDSLTRWSALDRGDGTAALSPRAAVSKGARLSGGSTVAEE
                     AAVLGKAALDGVSVLPRTVVPDGVSLRGAAVAQNLVVDPVSGATSLLTDLLPPAGTPR
                     GAGFGSRLAGLVLFLLSLPLWPVAFAWSFVANAGRPTRPYAFAGNGATPGTRAAVKTF
                     RFETAIPVFRDLPLLLAVLGGTLALAGVAPLAPEEEAGAGAATWAEARRQAPVGLLAR
                     SRMVVPASAPGEVARVVDAFDARRGCRGL"
BASE COUNT         2078 a         6439 c         6345 g         2336 t
ORIGIN      
        1 gcccctcccg ccgcggggga cgggcgcgcg tgggccgccg tcgtcctctc cggctcggcg
       61 gcgggcgacc tcttcttcgc ggcggagggc gcgctcgcgc ttcccgcggg cgtcgccgcc
      121 ctcctcgacg gcgccgggac ggcagacgaa ggtgcgccgc ggaggatcgc gcgcgaggct
      181 ctcaccggct ccgcggtccc gggcgtcacg ggcgcccggc tcctctgcac gagcgaggca
      241 cgctccgtcc tgcgcctcgg tccgccgccg gcggcgcgct gggcggtgga agggctgcgg
      301 agcgtcgcgg tcctcgcagg aaagctgacc ctcgtcgacg gcgaagaggc gcgcgacgtg
      361 cgcgcgggcg aggtggcgtt cgtcgcggaa cccgccgcga cgctccacgt ccaggcgggg
      421 aacgacgcgg ccgtcgcgat cggcttcggt cggccggagt tgtcggtccg gctcgggtag
      481 ccgtttcagc cgtcgcgccc cggggcgcga acgaggcggc cgaggagcgc ggagagcgca
      541 tcggcgctcg agcgaaaggc gaggtcgagc cggtcgatct cctccggcgc gaaccacccg
      601 acctcgagcg tctcgtcggc ggcgtacggc tccgggctcc cgggcaggag cgaggcgcga
      661 aaggccgcga cgatcaccgg ccggcccggg taggagtaga cgccgagcag cccgtcgatc
      721 cgcgcgagcg ccccgcactc ctcgcgcgtc tcgcgcagcg cggcctcctc gagcgtctcg
      781 ccccggtcga cgtggcctcc gggaaagacc catcgcccgt agcccggctc gatcccgcgc
      841 ctgaggagga ggacccggcc gtcgcggacg acgaggcagc cggcggcgac cttcgggtcg
      901 atccagtgga ccgtgccgca cgcgggacag acggggaccg tcctcccgtc gcccgctccg
      961 acggaacgct cctcgagccg ctgtccgcac tgcgagcaaa agagaggacg cgcgtacacg
     1021 gtccgagcct accgcggacc gcgtcgcggg cgccggcgcg gcgggaaccg tgcgctccgt
     1081 cacggaacgc gccctgctcg tcccgaacgg aggacggcgc gccttgacct cttcccggac
     1141 ggtggccaga atacgcggct cgaatggcgt gcccggaggt cgtgggatga gcaggcactg
     1201 gacgcaggac gaaatccgct ggctgaacgc caacgtggaa cggatggatc tcccgagcgt
     1261 ctcccgcgcg ctcggcgtgc ctctcgtcga ggtcgagagg aagctcgccg agctccgggc
     1321 ggacgcggcc gcggcccagc ggaagccgcc cgccacgcac cgggaggccg cccgcgagct
     1381 gacccacgcg cggaagctgt tcgagaaggg gatggagcac cttcaccgtc gcgagatgcc
     1441 ggcggccgcc gcctgcttct ccgagctcct cgagctccgg cccgacgagc gggagctcgt
     1501 cgaccgcgcg cggacgttcc tcgccgtcgc gcggaacggg aagtccgtga aggagaagcc
     1561 gctgcgcgac ccggccgaga tctaccacgc cgccgtgttc gagaagaacc gcgggaactg
     1621 cgcgacggcg atcgagctgg tccgctcggc ggacggccag gccgacccgg acgggcgcct
     1681 cgcctacctc gcggcgtgct gctacgccct ccacggccgc ccggaggagg cgctcgagag
     1741 cctgagggcg gcgatcgccg cctccgacca gaaccggatc caggcgcgcc tcgaggcgga
     1801 cctcgcgccg ctccggtcgc agcctcggtt cgagtcgctc gtcgggcggg cctgaccgtg
     1861 ccggccgcca ctgccgtcgt cctcctcgcc gccgggcggg gcgtccggat gaagtccgcc
     1921 cgcccgaagg tcctgcacga ggcggcgggg cgtgcgctcg tcgaccacgt cgtcgcggcg
     1981 gcgcgcacgt tcctcgcggg gcgccccggc tcgcggctcg tcgtcgtcgc gggcgcgggg
     2041 cgggacgagc tcgtcccgca cctcgcccgg acggcgcccg aggcgctcgt cgccgtccag
     2101 gaccctccgc gcggcaccgg cgacgccgtc cgtgtcgcgc tgtcggcgct cggcgacgcg
     2161 gcgcgcgtcg tcgtcctggc cggcgacgtc ccgctcctga ccgccgcctc gctcgcccgc
     2221 ctcgacgagg cgctggacgc cggggccggg atcgccgtcc tcaccgcgct cctgccggac
     2281 gcgggcgcct acgggcgcgt cgtgcgcgac gcggcggggc ggttcgaacg gatcgtcgag
     2341 gcgaaggacg cgagccccga ggtgcgggcg ctcggcgaga tcaactccgg catctacgcc
     2401 ttcgaccgcg agcttctcga gagggagctc ccgcgcctga cggacgccaa cgcccagaag
     2461 gaatactacc tgacggacgt cctcgggttc gccgtgggcg acggcgcggc cgtcgccgcg
     2521 ctcccggtcg acgacccggc cgaggtcctc ggcgtcaact cccggctcga gctcgccgag
     2581 gtcgacgcgc tcctccggct ccgcgccgcg cgcgccgcga tggccgccgg ggcgactctg
     2641 atccggcccg agacgatcac gctcgacgag ggcgtgacgt tcgggtcgga cgtcgtcgtc
     2701 gagccgttcg tgtcggtcac cggggcctcg tcggtgggcg ccgggacgcg gatcgggcag
     2761 gggtgcgtga tccacgcctc gacgatcggc gcgaacgttt ccgtccgccc ctactgcgtc
     2821 ctcgagaagg ccgccgtggg cgacggcgcg gtcgtcgggc cgttcgcccg cctgcgcgag
     2881 gggaccgacc tcggagaggg ggtccacgtc gggaacttcg tcgagacgaa gaaggcccgc
     2941 ctcgagaagg gggcgaaggc gaaccacctc acctacctcg gcgactgccg gatcggcgag
     3001 cggacgaacg tcggcgcggg cgtcatcacc tgcaactacg acggctacgg gaagcacttc
     3061 accgacgtcg gcgccggcgt cttcatcggc tccgacgtcc agctcgtcgc cccggtcgcg
     3121 gtcggcgacg gcgccctcgt cggcgccggc tcgacgatca cgaaggacgt ccccgccgac
     3181 gccctcgcca cgtctcgcgt cccgcagaag acgatcgagg ggggcggggc gaggtaccgg
     3241 gagcggaaga ccgccgcgaa ggcggctgga aagtgaagcc cgcgcggagg ggttcgggga
     3301 acgtgaggga gcgcgcagcg ctccttcacg aagacgaagt caacccggcg tccgggttgg
     3361 ctgcgccttc acctcgcggc caggccgctc ggtggtcctc cgagaacatg acgaaccgac
     3421 cgatggcgcc ccggcgttct tcgcgagcgc ggaacgaccg caggtcaatg ggcgattgag
     3481 gagtagttca tgtgcggcat cgtcggctac gttgggccgc gggaggcgac gccgatcctg
     3541 gtcgacggcc tgcggcggct cgagtaccgc ggctacgact cggccgggat cgccgtcctc
     3601 ggcgcggacg ggctcgtgcg cgtcacccgg agcgagggga agctcgggaa cctgacggcg
     3661 cggatcgagg cggagccccc ctcggggcgg accggcatcg gccacacccg atgggcgacg
     3721 cacggccgcc cctcggaaga gaacgcccac ccgcaccgag acgcctcggg gcgggtcgtc
     3781 gtcatccaca acgggatcat cgagaacttc ctgccgctga agagggagct cctcgcccgc
     3841 ggcgttccct tcacgtccga gaccgactcg gaggtcatcg cgcaggcgat cggggccgcg
     3901 tgcgccgacg ccccggaggc gccgttcggc gaggtcgtga ggacggtcct ctcgcggatg
     3961 acggggatgt acgccgtcgt cgtcctttct gccgacgagc cgggggtcct ctacgtcgcc
     4021 aagaacggcc cgccgatcgt cctcggcctc ggggcggacg agaacctcgt cgcctccgac
     4081 gcgacggcgc tcctctcgca cacccgcgac ctggtcttcc tcgaggacgg cgacttcgtc
     4141 cgcctcacgg cggcgtcggt ggcggtgacc gcgctcgacg gcgcgccgcg gagccggccg
     4201 gcccggcgtg tcccgtggga cgccgtggcc gcggagaagg ggggctaccc ccacttcatg
     4261 gcgaaggaga tcgccgagca gccgacggtc gtcgtggaga cggtggggca caagctctcg
     4321 ctcgagacgg ggcatttcag cgaggatgcg atgggggtct cgccgaagct cctcgcggac
     4381 gtcgaccggg tcgtcgtcgt cgcctgcggc acgagctggc acgccggcct cgtcgggaag
     4441 ttcctcctcg aggaggtcgc ccgggtcccg gtcgaggtcg actactcctc ggagttccgc
     4501 tatcggaaga acctcgtcgg gccgcggacg ctcgtcgtcg ggatctcgca gtcgggagag
     4561 acggccgaca ccgtcgccgc cctgaccgac gcgaagcggg ccggcgcgcc gacggtcggg
     4621 gtcgtgaacg tccccggctc ggcgatcgcg cgcctcgtcg acggcgtcct cgcgacgcac
     4681 gcggggccgg agatcggcgt cgcctcgacg aaggcgttca cgacgcagct cgtcgccctc
     4741 gccctcctcg cgtactacgc ccgcgaggcg cgcggcctcg gccgcctcga gccgggcgac
     4801 ccgttcctcg aagggctcgc ccacctcccc gtcgcgctcc agcaggcgct cgccctcgag
     4861 ccggcgatcg agaagctctc gcgcgccctc gcccacgccc gcgacgtcct ctacctcggg
     4921 cgcggcgcgc actacccggt ggcgctcgag ggggcgctca agctgaagga gatctcctac
     4981 atccacgccg aggggtaccc ggcgggggag atgaagcacg gcccgatcgc cctgatcgac
     5041 ggcgagctcc ccgtcgtcgc gctcgcgccg cgggacgccc tctacgacaa ggtcgcctcg
     5101 aaccttcgcg aggtgaaggc gcgcgaggga aggctcgtcg ccctcgtctc cccgggcgac
     5161 gaggacgttt cgacgttcgc cgacgagacg ctcgtcgtgc cggagatcca ccccgcgctc
     5221 cagccggtcg tctccgtcgt cccgctccag ctcctcgcct accacgtggc gcggcggcgg
     5281 ggctgcgacg tcgaccagcc ccggaacctc gccaagtcgg tgaccgtcga gtaggggctc
     5341 ggtcggctat tctcccggtc ccttgttgaa cgtccgcgag aaagccctcc tcgaaggcct
     5401 caacccggag caggcagccg ccgtctcggc cgggccgggg cccctcctcg tcctggccgg
     5461 cgccggctcc ggcaagaccc gcgtcctgac ccggcggatg gcctggctga tggcccacgg
     5521 cacccccgag ccttcgatcg tcgccctcac gttcacgaac aaggccgcgg gcgagatgaa
     5581 ggagcgtgtc gcgcacctcc tcggctcgcg gacgccccgc tcgttcgtcg ggacgttcca
     5641 cgcctacggc gtgcggctcc tccgccggtt cgcttccgag gcggggctga cgccgggctt
     5701 cgtcgtcttc gacgcggacg accagctcgc cgtcttccgg caggcgctca aggccgccgg
     5761 gatctccgac aaggcgctca ctccgaaggc ggtcgcctcg aagatctcga gggcgcgcaa
     5821 cgccggctgg agccgcgacg agtacccgcg ccgtttcggc gacttcgtcg ggacccgcct
     5881 cgccgagctc cacgcgacgt acgagaagga gctgaagaag gcgaacgcgg tcgacttcga
     5941 cgacctcctc gtgaggaccc gcggcctcct cgagaggagc ccggaggtcc tcgccctcct
     6001 ccggcggagc gtcacgcagc tcctcgtcga cgagtaccag gacacgaacg gcgcccaggc
     6061 cgggatcgtg aggctcctcg cgggggaggc acggaacgtc ttcgccgtcg gcgacgagga
     6121 ccagtcgatc taccgctggc gcggcgccga cgtctcgaac atcctggatt tcgagcggga
     6181 cttccccggg gcggagacga tccgcctcga gcggaactac cgctcgacgg cgccgatcct
     6241 cgacgccgcg ggggccgtcg tcgccgagaa ccgccgccgg ctcggaaaga gcctgaaggc
     6301 gacgaagccg ggcggggagc cggtgacgct cctcgtcctc cccgaggagc gggacgaggc
     6361 gcgcgaggtc gtcgagcgga tctcccggct gaaggcggtc aagccggggg cggaggtcgc
     6421 ggtcctcttc cggacgaacg cccagtcgcg ccccttcgag gacgagctcg tgcggaaccg
     6481 gatgccgtac ctcctcgtcg gggggacgcg cttctacgag cgggccgagg tgaaggacgc
     6541 cctcgcctac ctccgcctcc ttcgcaaccc cgaggacgac gtctccttcc ggcgcgtcgt
     6601 caacgtcccg gcccgcggca tcggcgcggt gacgctcgaa gggctctcgg ccgccgcggc
     6661 ggagtgcggg agctcgctct tcgcggtcct ctcggccctt cccgacggcc tgacggagcg
     6721 ggcgaggaag gcgctcgtcg agttccggga cctcgtctcc tccctcaggg aagagttcgc
     6781 ggccgaggat gcgggcgcag cggccgcggt cgcggccgtc ctctcgaaca ccggcctcct
     6841 cgcgctctac gaggggtcgg acgacccgca ggaccaggcc cggcgcgaga acctcgacga
     6901 gctcctcgcc gccgcccgcg agcacgagcg gtcgggtgcc gagggcgagg aggcggaccg
     6961 ttccctcgcc gcgttcctcg acgcggtgac gctccgctcc gacgcggacg acgtcgacga
     7021 gcggaagggg atcgtcctga tgacgctcca cgcggcgaag gggctcgagt tcgacgaggt
     7081 cttcctcgcc gggatggagg acgggtcgct cccgcacttc tcctcccggc aggacgagga
     7141 cgagctcgag gaggagcggc ggctcgccta cgtggggatg acgcgggcga aggagaagct
     7201 gaccctctcc cgcgtcctcc gccggatggt ccacggcgaa tggacgagcc gcgagccgtc
     7261 gccgttcctc gacgcgatcc ccgagcgggc gctcgccgtc gtcgaccgga cgttcggccc
     7321 ggggtcggcc gacggtttcc gcggcggagg gcgaggcgcc ggtctcttcc ccgactacga
     7381 aggggagtct caggagacct gggcgccgcc gcggtcggcg gcgcggccgg gctaccgccc
     7441 ggcggcgcgc gccgcgaagc ccgcgatccc gacgatgcgg cggacgccgc cgccgccgac
     7501 cgcctccggc ttccggcgcg gcgggcgcgt gcgccacccg gagtacggcg tcggggtcgt
     7561 ccttctcgtc gagggctcgg gcgacgggga gaagctgacg gtctacttcg accgcgcggg
     7621 gcggaagaag ttcgtcgcgc gcttctcgaa cctcgtcccc gcgtagccgg gcccgcgcct
     7681 tcggcgcccg gcgcgggaac cggccgtcag cgcggcggcg ccggctcctc gacggcggcc
     7741 tcgaggagcg cgcgctcggc gtcggcccag gcgcgctcgg cctcgaggag agcccgccag
     7801 cggcggcgcg ccgcggtgga gcccggcgac gccttcttca gcgcggcgac gagaagcgcg
     7861 cggtactcct tcaggcgggc ggcgcgcgcc tcgcgcggct gccgctcgag gaacgcgatc
     7921 cgcgcgagcg cggcgtcgcg cgggcggggc atcgcgagcg gctccgcgag ccactcgcga
     7981 agctcgcggc gtccggcggg cgtgatgcgg tactcgcggc gcggcggacc cttctccgag
     8041 cggcgcctct tcatcacgac gaatccgccg cgcagcagac ggcgcagcgc ggggtagatc
     8101 tgcgggagct cggcgtccca cccgagcgcg aggtcgcgcg ccatgcggct cttcaggtcc
     8161 catccgctcg ccggagcgcg cgacaggagc ccgaggagga cgtgcgggag cgacacggcg
     8221 aaagtataaa agtctttcca taccggggtg caaaacccgg aggattccct gtggaattcg
     8281 agagacgccg agggcggcga cgaggcgccc gtcgaggcaa aaagaaaggg ggcccgttcg
     8341 gcccccgtat cccggaaatc cttgaaaaat aaagagatgt gcctgaaagg cctacttctt
     8401 ggccttcttg gccggcttct tcgcggcctt cttcgcggcc ttcttggcag ccttcttcgt
     8461 cgccatggtt actgtcacct cctcttggca gaatcgattg acagtcccga aagccgcgtg
     8521 ctacgttgat cgtccctcga aggacggggg cccgcgacct acggccctgc cgactgcccg
     8581 tcagtcatga gttccagggt tccagggttt caggttttcc cgttcaccgg tgaagtgatt
     8641 gccgtcgctt cgccggtatt tttttctccg cagagaattg tagaagaacg agaaccggat
     8701 gcaaggattt ttttcttgac gcgagcggat tttcttttcc ccgcgagggc gctttcgcga
     8761 agtcgtcgat cgcgagccgc gttcgcgctc cgctcgcgcg cgagagcgcg gaacgggtag
     8821 catcccgcgc gatgtcgaac gtcgatgcct ctcctctcgt cctcgcccac tcgcccgact
     8881 ccgacgacgc gttcatgttc tacgggctgc gcgcggaagc ggtcgacacg gaaggcctcg
     8941 tcttccgtca cgagctcgcc gacatcgaga cgctgaacgg tcgcgccctc cggggcgagc
     9001 tcgcgatcac cgccgtgtcg ttccacgcgt acgcgcacct ggcgaagacg tacgtgctcc
     9061 tcccgcacgg tgcgtcgttc ggcgacggct acggcccgtg cgtgatcgcg cgcgaggccg
     9121 gcgacgcgcc cgcggccctc gaggcgctcg ccgggaagcg ggtcgccatc cccggagcgc
     9181 tcacgacggc ggcgctcgcg ctccggatgc gcgtcccgag cgctctcccc gtcgtcgtgc
     9241 cgttcgaccg cgtcggcgac gccgtcgccg cgcgggaggt cgaggccggc gtcgtcatcc
     9301 acgaggggca gctcacctgg aaggaggagg gcttccgcct tctcggcgac ctcggagccc
     9361 tctggacgaa ggagacgggg ctcccgctcc cgctcggggg gaacgcggtc cggcgggacc
     9421 tcggcgcgga gaagatcggc aggatctccc gcgcgctcgg acgctccatc gagtacgcgc
     9481 tcgcgaaccg cgaggcggcg ctcaccgacg ccatgcgttt cgcccgcggc ctcccgcgcg
     9541 agaaggccga ccgcttcgtc gggatgtacg tgaacgactg gacccgctcg tacggagagc
     9601 gggggcgcga ggccgtccgt ctccttctcg acagggcttt cgaggcgggc gtcgtcccga
     9661 cgcgcgtcga gcccgagtgg gtcgaggagc gatgagcctc gagcggttca cgcgcggctc
     9721 cgcgctccgg accgcgctcc tcctcgtcct cggggcgctc ctcggcgcgc tcgtcttctt
     9781 cgccggcgcc gccgggaccg tcctccggga gtccccctcc cgccacgcgg gaggggcggt
     9841 gaaccgagcg ctgctcgacc tgctcgacct ctcgacctac ggcgtggcgg cggtcgcgtt
     9901 cgtcctcgtc ctcgtcctcg accgcgtcga gccgttcggg aaggtgcaga aggggctcgc
     9961 cctccgcctc ctcgcggtcc tggccgcggc cgccttcgcc tcgcacgagg tgatcacgcc
    10021 gcagatgatg tcgctccgcg accggctccc gacgctgatc gacctcctgc cggcgggaga
    10081 cccggggcgg aaggcgtggg ggcggctcca cgggttctcg tccggcatcc tggtcgtccg
    10141 gatcgcgacc gccgcgttcc tcttcctcct gctcgcgagg aagcgccgcc ggacgatcgc
    10201 gacggcggag gtcccggcgg gcgtcccccc cgcggcctga gggcgaagcg gaggccggcc
    10261 ctcaggccgg cttcttcgcc gtccggccgc gcagggactc gaggcgccgg cgggcgtcgc
    10321 ggttctcggg ctcgagctcg acggcgcggc cgaacgccgc gacggctccc ttcacgtcgc
    10381 cggaggcggc gagcgcctcg ccgagggcgg cctgcgcgcg cgcgtcgcct ccctgcgagg
    10441 cgtcgacggc gctgcgggcg cgtgcgacgg ccgccgggag gtccttcagg cggacgagga
    10501 gagcggaggc gcgcacgagg agggccgggt cgttcgagac gcttccgagc gcgacctcga
    10561 gggtcttctt cgcctcgtcg ccctgccggg cgaaggcgag cgtctcggcg agggcaagga
    10621 ggcgggacgc gcgagcccag gcgccgccag aggccttctc gccgagcgcg acggcgcgcc
    10681 gcgccgcggc gagcgcggcc tccggcttcc cgctggcgcg ctccgcggcg gcgagcgcga
    10741 cgtgaaggtc ggcggcgagc ggcgcgtcct cggggaggag cttcagtccc gcgagcgcgg
    10801 aggcgcgggc ggccgcgccg ttccccgcgc gcgcctgcgc ctccgcggcg gcgaggtgca
    10861 ggatggcggc gcgcgggtcg gcggcgaggc gtggcgcgag cgattcgatc aggtcgagcg
    10921 tctcggccga gagagcgccg gcgccgtgct cgaggctgac gaggtagagg aacgcctcgc
    10981 gggccccctc ctccgcgagc gacgggtcct cctcgacggc gcggagcgcg atcgggcgga
    11041 gccgggcgag gtcgttgcgc tgggccagct gcgccgcgag gccgccggcg agcgccgggt
    11101 cccacgtctc cttcagcttc gcgatcgccg cggcgagggg cgtcttcccg gagaggatgg
    11161 cgtccgcggc gccgaggaac tcctgggggc cgaagacgcc ggagaggcgg tcgatctcgt
    11221 tcccggccgg gtcgatgaag aggactgtcg ggaaggcgcg gacggcgaag cgctgcgaga
    11281 tgcgccgccc ctcccccttc tcggcgtcga gcttcacggc cacgacgttc ttccgggccc
    11341 actcgcccac cttcgggtcg gcgaacgtca gccggtcgag ctgcttgcac gggccgcacc
    11401 agacggcata ggcgtcgagg aggaccggct tcttctcggc gcgcgcgcgg cgaagggcct
    11461 ccgcgaaggg ccgcccctcg gagaaggaga tcccctgcgc ccgggaggga gcggccggag
    11521 aggcgacgac ggcgagagcg aaggccgcga ggaggacgga acggcgcatg gggccggatg
    11581 taagcacgct ccgttcccga gggtcaaacg acgaacgcgc ctgcgcgccg ccgcggcggc
    11641 tcagaacgcg gggcggagag ggtggggcgg ctcctcgacg ggctcgccgg ggtatcccac
    11701 ggactcgagg aagaggccgg acggcggaac ggtgagccgc gccacctcgc tcgtccgctc
    11761 ggcgaggagg cgcgcgacct gggcctcggc catccgtccg gcgccgacct cgagcaggac
    11821 gccgacgacg cgccggacca tcttccagag gaagtgagac gcggcgatcc ggacgaggac
    11881 gagaccgtcg gcctcggcgg gcgcggcgac gcgcgcctcc tcgacgacga cgatcgtcga
    11941 gtcgtgcccc tcggggttct cgcagaagcc ggcgaagtcg tgccttccgg cgaggagccg
    12001 cgcggcgcgg tccatcgcgg tgacgtcgag gtcgtccttc acccagtaga cgaacggctt
    12061 gccgaacgcg gtgcgccgag tcgcgatccg gtaccggtag acgcggcgga ccgcgtcgtg
    12121 cctcgcgtgg aaggtggccg ccgcgggagc gagcgcgagg acgttcacgt cgaaggggag
    12181 gagctcgttc cagcgccgga gggccgcggc ggcgtcgacg cgtgcacgcg gcgggaggtg
    12241 gacggaggcc acctggccga gggcgtggac gccggcgtcg gtccgccccg ctccgtggac
    12301 gcggacgccg ggaccccaga gctcccgggc cgcggcgagg aggacgccct ggacggtgcg
    12361 ggcgttcacc tgggcctgcc agccgcggta gcggcttccg tcgtactcga tcttcgcgcg
    12421 gaggaccgcg cccgccggcg cggaggtctt cgaaggggcg cggctcggga tcacgcggtc
    12481 attgtcgcag gccggaggcg cggggctcta tgatcctcga gagcctcgat gagaaggcgc
    12541 cggacgatcc acctctccgt cgaggcggac ttcccttcgg tccgcctcct ggcggccgcc
    12601 tgccacggcg tcctcgccga gtgggggctc ccgaccgagc ggaggtcgct cctggagctc
    12661 gcggtggtgg aggcggcgac gaacgtcgtg cgccacgcct accgcgggca tggcggccgg
    12721 gtcgaggtcg agctgacgcg cgaagggcgg gccatcgcgc tttccgtcgt cgacaccggc
    12781 atctccttcg acccgacgcg cgtcccgccc ccgcgtgacc tcgacccgga cgacccgtcg
    12841 acctggccgg agggagggat ggggctgccg atcatccgcg cggcctgcga cgagcttcgc
    12901 tacagctcgg acggcgcgag aaacgagctg acgctgcggc tgacggtcga cgagcccgag
    12961 gagccgtgac cggcgcggac cgccccttca ccgccgcggc cgtccccgcc gtcgagcggc
    13021 gctgtcggga gaccctcgcg gcgctcctct cgcgcctcgg ggaggtggcc ggcgacggtc
    13081 tcgccggggc ggcgctcggc ggggccctcg ggaggggcga ggcgccgacg acggtcgacg
    13141 cgaacggggc cttcgtcacg cccgttccgt tcgagctcct cgtcgtcctc gacgccacgc
    13201 cggggcgcgc cgcgtccgtc gctccccggc tccggcgcga gctggcggcg acggcgcgga
    13261 cgcgatcggc cgacgtccgt ctcgccaccg cggcccgttc ggcgctcgcc cagctcccgc
    13321 cgtcgatcgc gaacgtggag acggccgcgg ccgcccgcgt cgtcgcgggc cccgcggcgc
    13381 tcctcgctcc gctcgcgcct ctctcgaccg cgcggcccgg cgcggacgag gcgctcgctc
    13441 tcctcgtccg ccgcggggcg tcgctccttc tcgccgagcg cgccctctcc cgccgtgccc
    13501 cttcgcgcgc cgccctcctc gcggggcagg ccgccgtgca cgcggcggac ctcgccctcg
    13561 gaaccgcgct cctcgtctcg gccggccgct tccgtccgac cgacgccgcg cgcgacgccg
    13621 agctccgcct gctcgccgcg cccgcggggg acggcccggc ccccgcgggc ttccatctcc
    13681 ggatgtcgcg gacgcgcttc cgggacgtcg tcgaacggca tcgggccgcc gtggaggccg
    13741 cgcgcgaggt ggacctgccg gactccctcg gcgaggtgcg ggcgcaggcg gggcgggcga
    13801 ccgaccggtt cgtggaggtc ctccgcctcc tcgaggagga gcggctctcg cgtccgctgc
    13861 cgagctggac cgactacgtc cgcgcgctgg ccgagaggaa cggcggctct ccggggccgg
    13921 gactcttcga ggcgagccgc gaggaggagc tcccgtcgcg ccggagcgtc cgcgcctggc
    13981 cggttgccga gcggctcgcg ccggccctcg cgacgctcct cgactgggac ccgggcgacc
    14041 tcgccgtcgt gccgctcctc ctcgaccttc ccgcagacgc gacgaaggag gcgctcggcg
    14101 cgcgcctcgc atcgctcgcc gcctccgcct gacggagggg ccgtccggcc cggggccgta
    14161 tactccgccg ccgtgacgtc cgtgaccgag atggcggacg acccgggggc gtccggacgt
    14221 ctcctgttcg tcctggccct cctcgcgggc ctcctcctcc ggctcgtgca gctcggctcg
    14281 cccgatctcc tggggcccga agaggggccc tgggccgtcg gcgcccgaaa catcgtcgag
    14341 ggagggctcg cccagctcct cgcgctctcg tcgacgccgc tcggcgagcc gaccggcatc
    14401 ccgatcttct tcccggcgct cctggcggcg gcggtcaaga ccttcggcgc ctacgagtgg
    14461 gccatccgtc tcccctccgt cctgttcggc ctcgtcgccg cgttcgtcct cgagcggatc
    14521 gtgaggcgcg gcttcggcca gcccgccggg cacgtcgccg gcgcgttcgc ggcgctcgcc
    14581 tcgccgctcg tcctctccag ccggacggcg acggtcgagc cgacgctgac gctcctcggc
    14641 ctcgggggga tcatcttcgg cctccgggcg ttcgaggagg acagcccggg cgaggcgctg
    14701 ccggccggaa tcctcttcgg cctcggcttc ctgacgaagg gctacgccgt cggcctcttc
    14761 gtcatcccga tcctcctcgc gctgctgttc cgccctcgtc ttttcaccct cggtcggacg
    14821 aagcggaccc tcgcgctcct cgccgtctcg ttcctgctga cggccggctc acacctcctc
    14881 ctgatggcgc tcgtccaccc gacctccctg gggccgcacc tgtcccgcgc gttcgggagc
    14941 gcgcccgaca cgctccggca gctcaccgac gtcgcgctcg tgagcgccga cctccggatg
    15001 atcgtcaagt ccctcttcgt cctcctcccg ctcgtcgggg tcggcctcgc gtacctcgcg
    15061 cgacccctcg gcgaggagga ggtcgtctcg ggggcgacgg ggggcgagag gcgcctctcg
    15121 cacatggcgc tctggtgcgc gtacggggtc gagctcgtcg tcctggtcgc ggtggcgggc
    15181 tcgctcgagc tctcctccac gccggtcctc cccgccctct cggcgctcgc cggcctcgga
    15241 ggggcggccc tcctgacacg cccgcgctcg ccccggcggg tccggttcga gacgatcgcg
    15301 accgtcgcct ccggggcggc cgtcctcgtc ctggcgatcc tcctcgtctg gacggcgcgg
    15361 gagcctctct tcaacggggg ccgggcggcg ccgttcaccg ccgccggagc gctcgcggcg
    15421 atcgccctct cgacggccgg ggccgcgtgg ctcgtctcgg gcgacgcgga gcgccgcttc
    15481 ggacgacgcg ccgggctggt cttcctctcg gtcctcctcg tctccgaggg gctcgccgcg
    15541 gcgcgcgcga tccgtcacga cctcctcgcg caccggacgg gggtccgggc gctctccgac
    15601 caggttgcgc ccgagctcct tccgctctcc ccccgcgtcg tcgccttccg cgcgcccgag
    15661 ccgtcggcga tcgcgttccg gctcttccgg accgggacga cctgggccga ccggcccacg
    15721 gtggagcagg tcgccgcgga ggcgcgcggc ggcaagacgg tcgtctgggc ctggcgcgag
    15781 gggacgaccg atccggtcgc cgcgccggcg ccgctcgtga gggcgtggct catcgtgaat
    15841 acgcgtgagg tgacggccga ggtggacgcc cgcgccgggc gcacgacggg gctgcgcgcc
    15901 ttcgtcgccc gccctccgtc ataatcgacg accctatgac gaccgttctc ctcgcggacc
    15961 gcgatggccg cgccctcggc ccgctcgaag acaagaccgt ccccgccctc ctcccgctgc
    16021 gcggcgcgcc gctgctcgag cgcgcgctcg aggcgctcgt ctcggccggc gtccgctcgg
    16081 ccctcgtcgt cgtcggcccg cgcggccccg agatcgagaa gcgcttcggg aaggggattc
    16141 gatgggggat cgccctcgag tacgtccgcc gcgccgagga cgagacgacc ggggccgtcc
    16201 tgcgccggct cgagcatcgc ctcgacggcg agacgctcgt cctgcgcggc gacgcggcga
    16261 tcgagggggc gttcggggag ttcctccgcc gctccgcgac gagcgaggag cccgtcgtcg
    16321 ccgcgctctc cggagagcgc ctgctcggaa tgtggcgcgt ccggccggag gcgctgaaaa
    16381 agctggagat cccccgcgag ccggcggacg agagctgggt ccgggagaag ggccacgccc
    16441 cgctcgacgt cgacctggac cttgccccgc tcgactccct cacccgctgg agcgccctcg
    16501 accgcggaga cggaaccgcc gctctttccc cgcgcgccgc cgtttcgaag ggagcccggc
    16561 tctccggcgg ctcgaccgtc gccgaggagg ccgcggtcct cgggaaggcg gcgctcgacg
    16621 gcgtctcggt ccttccgcgc accgtcgtcc ccgacggcgt ctccctccgc ggcgccgcgg
    16681 tggcgcagaa cctcgtcgtc gacccggtct cgggcgcgac gtcgctcctg acggacctcc
    16741 tccctcccgc cggcacgccg cgcggggccg ggttcggaag ccgtctcgcg ggcctcgtcc
    16801 tcttcctcct gtcgctgcct ctctggccgg tcgcgttcgc ctggtcgttc gtcgcgaatg
    16861 cggggcggcc gacccggccc tacgccttcg ccgggaacgg cgcgacgccc gggactcggg
    16921 ccgcggtgaa gaccttccgg ttcgagacgg cgatccccgt cttccgggac ctcccgcttc
    16981 ttctcgccgt cctgggcggt actctcgccc tcgccggggt agcgcccctc gcgccggagg
    17041 aggaggcggg tgccggagcg gcgacctggg ccgaggcgcg gcgccaggcg ccggtcgggc
    17101 tcctcgcgcg ctcgcggatg gtcgtccccg cgtcggctcc cggcgaggtc gcccgcgtcg
    17161 tcgacgcgtt cgacgcgcgc cgcggctgcc gcgggctg
//
LOCUS       DAOTEZ010000032        17184 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2256_length_17184_cov_5.974079, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000032 DAOTEZ010000000
VERSION     DAOTEZ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17184
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2256_length_17184_cov_5.974079"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..643)
                     /locus_tag="P5164_02865"
     CDS             complement(212..643)
                     /locus_tag="P5164_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY42866.1"
                     /translation="MPDAVRILLVEDDDDDAELALRALARSEAGPRVVRARDGEEALD
                     VLLGRGPFAGGEAPRLDLVLLDLKLPKVDGIEVLRRFRADGRSRSVPVVVLTSSMLPA
                     DVRACYEAGANGFVVKQVEFAEHARNLRQLADYWLRINVRP"
     gene            complement(636..2438)
                     /locus_tag="P5164_02870"
     CDS             complement(636..2438)
                     /locus_tag="P5164_02870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY42867.1"
                     /translation="MTLDVRTLLALFAFTQALQVVVLSVQYAVFRSYRGTGAFLLWST
                     AVGIGTVAFLLRDDPTIGRAAIFAQNGLIVFGSLCLWAGFCRFHGRVEDRRLFAAVLV
                     PFLFAHATFIWVVPDIDLRAVALCVALVATALLSARELWRARTGESRPVALVLMAGLL
                     AHAAFFTARGALLLAGARVDDVWGGAAFNVAPYVDGFLLTNLVTFGLVILVNRRVNAE
                     VREARDHFRRLLDTIPDAVLITRVADGICRDANTGFETLTGFRQEEVVGRSSAELGIW
                     TDPRARDRVLERVRASEPVRDLELEGRRRDGTPFVGNVSSRLVDVRGEPHVLSVVHDV
                     TERNAAREALEKSAREIRELNAGLERRVAERTAELTDKNRELEAFVHSIAHDLRAPLR
                     AIDGFSGILEEEHAARLGDEGRRLLARVRGGARRMDRLLRDLVEYARAGSAGLRHERV
                     DLGLLARSAFADVAPADGRGVCRFVVGELPAVRGDAALLRVVLRHLLANAVKYTAERP
                     ASRVDVRGRVEGDVVVCEVADDGVGFDPAHTGRLFRLFERLHAGEGDDGTGIGLAIVR
                     RIVERHGGSVRAEGRPGQGATFTFSLPAWGEVDA"
     gene            complement(2525..3853)
                     /locus_tag="P5164_02875"
     CDS             complement(2525..3853)
                     /locus_tag="P5164_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42868.1"
                     /translation="MSSRTAFPRVFGEIVHDSFSVTSFFTGLLAALPTGLVLLFAASG
                     PRSKGDAAAVLVVWMGTVLVFVSRFAQVGVAGDTEGGLFSSAAGPWGDTLLAATRLLA
                     LWVAWTLPFVAWAFLKGGEPGAEVPGFGLVPYGLPRLPLLLSLWSGLGVVLSFAVVAA
                     AAAAPGFGALFSPALWRTLFGGRLGELFLAIAGVFGPPLTVLLVVLPVFGAVAPRQPK
                     LALTAFVPLLLYAVGMVLTLQGKLCGAFAAASLAEDDLEPIPDEAAPQAVTPSVAPGA
                     SERAAPAAGDPESLHAIWQARLAAGDLPGTLEAARAAIPAGLAKGSPKAAAEIYRQHL
                     DRLDALGLERPALSLLADQLLRDGDVAAAAWTYSQALDAEPGDAKAFKGLLRVAEHHL
                     EKAKAPLEAVRVYRYLLERAPGSPLAEHARDLLADVERRAAREPSSPVRP"
     gene            3998..4834
                     /locus_tag="P5164_02880"
     CDS             3998..4834
                     /locus_tag="P5164_02880"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HRY42869.1"
                     /translation="MKLRPTLLLAAAALATPAFAQFETPQPSPKATLSQRIGLTDVTI
                     SYSRPSVRGRAIWGDLVPYDTPWRTGANAATTLTFSDDVTIEGQKLAAGTYSLVTIPG
                     KDDWTVVFNTDTKLWWETQYDAAKDALRVKVKPEAAPMTETLHIGFPSISATGAVLAI
                     EWEKVRVPVKVGVEVDGKLKKALGAAEAGAWQSPLMAARYYWEQRQDRPEAWKQLEKS
                     ISAAKNWANVSRKARFLAEEGKTAEAVKAGEEALALAKADPAKPNTAAFEKSLGEWKA
                     KK"
     gene            4993..5487
                     /locus_tag="P5164_02885"
     CDS             4993..5487
                     /locus_tag="P5164_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRY42870.1"
                     /translation="MTQKSQAPGTMPKAHRLEREQLLPGTPAEVFPFFADARNLEALT
                     PSFLGFRILTPLPIEMREGTLIDYSLSLFGVPVRWKTRISAWEPGVRFVDEQLSGPYA
                     LWRHTHSFERRGDTTLMRDVVDYREPFGPLGTIAHHLFVARTVERIFDYRREAVDRLL
                     RRAA"
     gene            5484..5888
                     /locus_tag="P5164_02890"
     CDS             5484..5888
                     /locus_tag="P5164_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HRY42871.1"
                     /translation="MSAPAADLELFYDGACPLCAREARALRRLDRRGRLTLTDLAAPG
                     FDAGETGVPMERLLARIHGRLPDGTIVEGVEVFRKAYEAVGFRLLVAPTRLPGLSWIL
                     DRAYDVFARNRLRLTGRCEDDACRRGAPTAPA"
     gene            complement(5872..6447)
                     /locus_tag="P5164_02895"
     CDS             complement(5872..6447)
                     /locus_tag="P5164_02895"
                     /inference="COORDINATES: protein motif:HMM:NF027567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRY42872.1"
                     /translation="MASKARRMLRLPILALAALLASGASFADEKAAKPATPATRDELV
                     AAARKVMTSQTYCALITLDAAGRPSVRTMNPFPPEDDLTVWFATNDRSRKVAEMRADP
                     RVTLYYADHAKASGYVSLSGRAVLVDDRAEILKRKRAYWDQSFPGLKNLVLVKVVPEK
                     LDVLNYEGKIVVDPVTWRTPSIDVGAVTPAP"
     gene            complement(6535..8247)
                     /locus_tag="P5164_02900"
     CDS             complement(6535..8247)
                     /locus_tag="P5164_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRY42873.1"
                     /translation="MKGLDWAVLGASLLFIVGYGTWRSRKQKGLEGYLLAGRSQAWWT
                     VALSIMATQASAITFLSTPGQAFADGMRFVQFYFGLPIAMVILCVTVVPLFHRLKVYT
                     AYEYLEGRFDAKTRTLTASLFLLQRGLACGLTIYAPALVLSVLLGWSIQATTLVIGSL
                     VVVYTATGGAKAVNETQTWQMVVILLAMGFALAALLRVLPEGVGFGDAVLVSGALGRM
                     NAVETTFDLGDRYNLWSGLIGGAFLALSYFGTDQSQVQRYLTGRSVAQSRLGLLANGL
                     VKVPMQFAILFVGAMVFAFYQFVAPPLWFNPVESRKAAEARGAEMAALETAHAAAFEE
                     RRVEALRLVAAGRSGDEAQLAAAKESLRAAHGKSEAVKAKAAALVKEAVPKGEGKDTN
                     YVFLRFVLEVLPTGVVGLVLAAIFAASMSSTSAELNALSTTSVVDVYRRYLGKEASDA
                     KLVSVSKWLTVAWGAFAIVFAQYAGRLGTLVEAVNILGSFFYGTILGVFLTAFYLPKV
                     KGGAAFWGALAGEAAVVGAWLLTDVSFLWLNALGCAVVVAVALGFSAVRGAPGGASRE
                     TASA"
     gene            complement(8244..8378)
                     /locus_tag="P5164_02905"
     CDS             complement(8244..8378)
                     /locus_tag="P5164_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42874.1"
                     /translation="MADGGPVADEPPPVGKRWGVLYAVVLGALALEVLLFWLFAESFR
                     "
     gene            complement(8371..10965)
                     /locus_tag="P5164_02910"
     CDS             complement(8371..10965)
                     /locus_tag="P5164_02910"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRY42875.1"
                     /translation="MTRRSARLGAPLAVAFLALAAAPAAAAAPFGPERPAPAGELLLK
                     LKKLNVLGSVLYVAAHPDDENTAMIAAFAEGRLMRTAYLSVTRGDGGQNLIGSELGPA
                     LGLVRTHELLAARRIDGGEQMFTRAIDFGYSKTAEETFRVWGREEVLRDVVRAIRTFR
                     PDVVLARFPENGDGGHGHHTASAVLAREAFRAAGDATRFPEQIEEGLSPWQPRRLFWN
                     GWRRDGKPIEGSIPYEIGDYSPALGRSHTETAAESRSQHKSQGFGSSERRGRLANALI
                     LLEGDAVTADPFEGIDTTWARVPGGAHVGRLLAEAERLFDAREPSRVLPKLLEAHAAL
                     RGLGADPWIEGKRRELLAAIRSASGLWLEAIAASPSATPGSAFRLNVTALVRAGTPAT
                     LARVVVPWGTGEGLPSGAELKLNEPVSGSASVPVPVDAEATQPHWLRARPSRGLYAVP
                     APRDAIRPDGASLRVSFDVVMAGETLTYEEPVYFRRTDPVKGEEIRPFVVEPPVSVEI
                     EEEVLVLPDHAPRVVKVTVRAAELPKDRSGPPPLTPSIVASATGADGPAGEAPPPKVD
                     GAVRLSVPAGWRAEPAVRPFALAPGASATFEFTLRSPETPAVATIAAEAATGGDSWGL
                     TKRRIEYPHLPPLTYFERAEARAVHVDLARGRSRVGYVSGPGDEVPGVLRQMGYAVTL
                     LSADDLLRGDLARFDAVVLGIRAANTRAELKDAMPRLFDWVEAGGTLVLQYNTSFERV
                     TDRLGPFPLELGRDRVTDEESKVTLLAPEHPLLTTPNRIGPADFEGWVQERGLYFPAT
                     WDERYVPLLGMADPGEKESKGALLAARHGKGTYVYTGLSFFRQLPAGVPGAIRLFANL
                     VSGGTPRG"
     gene            complement(11045..13165)
                     /gene="glgX"
                     /locus_tag="P5164_02915"
     CDS             complement(11045..13165)
                     /gene="glgX"
                     /locus_tag="P5164_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017715355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HRY42876.1"
                     /translation="MSSSSPSGRFLDYISADFAVLRGHPIPFGATPSWRGVNFSVFAR
                     HATSVTLALFAPGDATPAAEFPLDPRYNRTGDVWHAFLRGLDPSVCWAWRAERATAPG
                     APWHRYGPELLLDPYARAIAGGEAWGEPPRPGEEKGSESVRARRGVILSREFDWGFDQ
                     PIDRHLADSVIYELHVRSFTRHSSSGVAHPGTFRGLVEKVPYLVDLGVTAVELMPVTE
                     FEENDNDRRNPFTGERLKNLWGYHPISFFAPRSAYAASAGPGAAVDEFREMVKALHEA
                     GIEVILDMVFNHTGEGDERGPTWSFRGLDNSTYYILDAEGRYANFSGTGNTFNCNHPV
                     VRDLILDSLAWWVTEMHVDGFRFDLASILGRGRDGSVLTNPPLLERIAADPVLANTKL
                     IAEAWDAAGLYQVGHFPNWGRWAEWNGRYRDDLRRFLRGDGGMVPLLATRLSGSADLY
                     QGGGRAPHHSINFVTSHDGFPLADLFRYSHKYNEANGEGNRDGGDDGNSWNCGHEGPG
                     GPPEVERLRRRMARNAFALLLVSQGVPMILAGDEAGRTQGGNNNAYCQDNEVSWLDWR
                     LVEENADLVRFVRTLIRFRRGSPLLRRRSFVPEGPGSAPVTWHGHVPFRPDWSPGATC
                     LGMQLKDPAQGDDVFVVANAHHEAHRIELPPASEGRRWHVVADTYREPPADVFEQGAE
                     PLVADPLRVEVGPRSVVILTGKRT"
     gene            13286..13690
                     /locus_tag="P5164_02920"
     CDS             13286..13690
                     /locus_tag="P5164_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42877.1"
                     /translation="MKQKTLTATLVLAAFVSFPVAATGQKSEAPGPAPAAEAAMKIHV
                     DPATGQVVAPKADPQAAGPRTTAAAVEAFPPTRVEKVTTKAGGKKVALHGRFMMEMTA
                     KVGADGKVSHGCEAVEETGAKPAPAKEHRHDR"
     gene            13680..15371
                     /locus_tag="P5164_02925"
     CDS             13680..15371
                     /locus_tag="P5164_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42878.1"
                     /translation="MTARALLGGTLAAALALAAPAAATTITIINVDSSGVGFNDPAPR
                     TPVGGNNGTTLGEQRLNVFKYAAAFWAARVDSPVEITVEAKFEAKACDASTATLGSAG
                     TRYIFSDFKGAPRAGTWYSSALANKLAGEDLEPAEADIAASFTTALDDGSCLFPKKWY
                     YGLDAQPTGSTTDFATVVIHELGHGLGFQTFVSRTTGKKFSGDDNPGLDDAYMVHLYD
                     GSTGKTWDQMTDDERLASGTNTGNLLWNGSNVVAVASGVLIQGVGAGGRPLMYAPNPS
                     QAGSSVSHWDTTLTPNEMMEPYATQAMHRLDITDELMLDLGWGLASGSTEYHWILPSS
                     AKAPGKGGAYYTTALSIGNRGAAEARYRMKFLGHDADGTGGAESSEFVLGPNQSVTYE
                     DVLGSVFGLANGNYGAIRVTANVPSLNVLGQTATPVLTDWKSCVSSGFFGQSVPAVPA
                     SGLITAGTTRSIVGVRDDAAFRTNLILANAGTSIVTVDLTLLSSSGAILGTRTVSLHP
                     LGMTQIGPLVGGMGVTGAVRDAQLLLTTTTTGGAFAAYASVIDNVTNDPRTLLPN"
     gene            complement(15461..17098)
                     /locus_tag="P5164_02930"
     CDS             complement(15461..17098)
                     /locus_tag="P5164_02930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY42879.1"
                     /translation="MTRPSRLVLFGLPGLLLLTAVLAVLDARRDGVTADEPIHVAAGV
                     LQARHGSWAVNVELPPLAKELQGRAALHAGAVSSPPLSFRTLFLSARAQLFGAADPLK
                     VLFAARLVTVAFLVLLLLFASRAAGGGEAGLLAAALLAGQTALLPHGHLVTADVPAAA
                     LVVAAVWGALALYEKPTALRLLGTALAAAAAIATKQTALVLLPLLPLLVVPALRMSRD
                     RTVTRRFVLVALGLPVLTIALLSLILGRFAATEAAVLPTLLRLYQLPAADADLVTRIA
                     GLDEGLGRYALSTLFVLRQTGAGRLSYFLGDVSPDPGPAYHLVALLVKSPLPWLLVVV
                     AGLLAVLVRAPLRARLLLFSAGVFLAASLAGPKIGVRHLAPVIVLLSAGAAAALGEPL
                     RRRSPAGYAVVLLLVLSPLAIGRSVGRHSITARLFPEPPLADSNLDWGQDLPRLAALS
                     ASRGIAPGDLGVAYFGGDLPSFRVPGCVDLLAADAPLPRYVAVSRQLLLVGPETVPFV
                     DGRPRAARVLAELKVRGARAVARAGDSIELFELPAPR"
BASE COUNT         2605 a         6230 c         6254 g         2095 t
ORIGIN      
        1 gagcacgagt acgactcgct cgagcagatg cgcggttcga tgaacctcct ccgctgcccc
       61 gacgcctcga tgttcgagcg cgccaactac atccgcatcc tgcagggcta caaggtctga
      121 cggccggcac acccgtgtcg atcgggaaag ggggcggccc gcgggccgcc ccctttttcg
      181 tccccgggcc gcgccgcgag agggggagcc ctcaggggcg gacgttgatc cggagccagt
      241 agtcggcgag ctgccggagg ttccgcgcgt gctccgcgaa ctccacctgc ttgacgacga
      301 agccgttcgc tcccgcctcg tagcaggcgc ggacgtccgc cgggagcatc gacgaggtca
      361 ggacgacgac cggcacggaa cggcttcgcc cgtcggcacg gaagcgcctc aggacctcga
      421 tcccgtcgac cttcggaagc ttcaggtcga gcaggacgag gtcgagccgc ggggcttcgc
      481 ccccggcgaa cggcccgcgc cccaggagga cgtcgagggc ctcctccccg tcccgggcgc
      541 ggacgaccct cggcccggcc tcgctccgcg cgagggcgcg gagcgccagc tccgcgtcgt
      601 cgtcgtcgtc ctcgacgagg agtatccgaa cggcatcagg catcgacttc cccccaggcc
      661 ggaagagaga acgtgaaggt cgccccctgg cccgggcgtc cctccgcgcg cacgcttccc
      721 ccgtgcctct cgacgattcg ccggacgatt gcgaggccga tcccggtacc gtcgtcgcct
      781 tcgccggcgt gaaggcgctc gaagagccgg aacagccgcc cggtgtgggc cgggtcgaag
      841 ccgacgccgt cgtccgcgac ctcgcagacg acgacgtccc cttcgactcg cccgcgcaca
      901 tccacgcggc tcgcaggcct ctcggcggtg tacttcaccg cgttcgcgag gagatgccgg
      961 aggacgacgc ggagcagcgc cgcgtccccg cgcacggccg gaagctctcc gaccacgaat
     1021 cggcagactc cgcgcccgtc ggccggggcg acgtcggcga acgcgctccg cgcgaggagg
     1081 ccgaggtcga cccgctcgtg ccgaagtccg gcggagccgg cccgggcgta ctcgaccagg
     1141 tccctgagga gccggtccat ccgccgggct ccgccccgca cccgcgcgag gagtcgccgc
     1201 ccctcgtccc cgaggcgggc cgcgtgctcc tcctcgagga tccccgagaa gccgtcgatc
     1261 gcccggagcg gggcgcggag gtcgtgcgcg atggagtgga cgaacgcctc gagctcccgg
     1321 ttcttgtcgg tcagctcggc cgtcctctcg gccacgcgcc gctccagccc cgcattcagc
     1381 tcccggatct cccgcgcgct cttctcgagg gcctcgcggg ccgcgttccg ttccgtcacg
     1441 tcgtgcacga ccgagaggac atgcggctcc ccccggacgt cgaccagccg cgacgagacg
     1501 tttccgacga acggtgtccc gtcgcggcgc ctgccctcca gctcgaggtc ccggaccggc
     1561 tccgacgccc ggacccgctc caggacacgg tcgcgggccc gcgggtcggt ccagatcccc
     1621 agctccgccg aggagcggcc gacgacctcc tcctgccgaa agcccgtgag cgtctcgaag
     1681 cccgtgttcg cgtcgcggca gatcccgtcc gccacgcgcg tgatgagcac cgcgtcgggg
     1741 atcgtgtcga ggaggcgccg gaagtggtcg cgcgcctccc ggacctcggc gttcacgcgc
     1801 cggttcacga ggatgacgag gccgaacgtc acgaggttcg tcaggaggaa cccgtcgacg
     1861 tacggcgcga cgttgaaggc ggcgcccccc cagacgtcgt cgacgcgggc cccggcgagg
     1921 aggagcgcgc cgcgagcggt gaagaacgcc gcgtgcgcga ggaggcccgc catgagcacg
     1981 agcgcgacgg gccggctttc gcccgtccgc gcgcgccaga gctccctcgc cgagaggagc
     2041 gccgtggcga cgagcgccac gcaaagagcg acggcccgga ggtcgatgtc cgggacgacc
     2101 caaatgaagg tggcgtgcgc gaacagaaag gggacgagga ccgccgcgaa gaggcgccgg
     2161 tcctcgactc gcccgtggaa tcggcagaag ccggcccaga ggcagagcga cccgaagacg
     2221 atcagcccgt tctgcgcgaa gatggccgcc cggccgatcg tcgggtcgtc ccggaggagg
     2281 aacgcgaccg tcccgatgcc gacggcggtg ctccagagga ggaacgcgcc ggtgccgcgg
     2341 tagcttcgga agactgcgta ctggaccgag aggacgacga cctggagggc ctgtgtgaag
     2401 gcgaagagcg cgaggagcgt gcggacgtcg agagtcacgg ccgccggccc tccgaccctg
     2461 cgaacgccgc gaacgccgcg aacgccgcga acgcctccga gcctcctctc aacacgcccc
     2521 ccgattatgg acggaccgga gatgacggct cccgcgcggc ccgtcgctcg acgtcggcca
     2581 ggaggtcgcg ggcgtgctcg gccaggggcg agcccggggc ccgctcgagc aggtagcggt
     2641 agacccggac cgcctcgagc ggggccttcg ccttctccag gtggtgctcg gccacccgga
     2701 gcagcccctt gaacgccttc gcgtcgcccg gctccgcgtc gagcgcctgg gaatacgtcc
     2761 acgccgcagc cgcgacgtcc ccgtcgcgca ggagctggtc ggcgagcagg gagagcgccg
     2821 gcctttcgag gccgagcgcg tcgaggcggt cgaggtgctg ccggtagatc tccgcggccg
     2881 ccttcggcga gcccttcgcc aggccggccg ggatcgccgc ccgcgcggcc tcgagcgtgc
     2941 ccggaaggtc tcccgcggcg aggcgcgcct gccagatcgc gtgaagcgac tccgggtccc
     3001 ccgccgcggg cgcggcccgc tccgacgcgc ccggcgcgac cgagggcgtc acggcctgcg
     3061 gagcggcttc gtccgggatc ggctcgaggt catcctccgc gaggctcgcg gccgcgaagg
     3121 cgccgcagag cttcccctgg agcgtcagca ccatcccgac ggcgtagagc agcaggggga
     3181 cgaaggcggt gagggcgagc ttcggctgcc gcggcgcgac cgcgccgaag acgggcagga
     3241 cgacgaggag gaccgtcagg ggcggaccga agacgccggc gatcgcgagg aacagctctc
     3301 ccagacgccc cccgaacagg gtccgccaga gcgccggcga gaagagcgca ccgaagccgg
     3361 gagcggccgc cgccgcggcg acgaccgcga aggagaggac gacgccgagg cccgaccaga
     3421 gcgacaggag gagcgggagg cgcggaaggc cgtatgggac gaggccgaaa cccggcactt
     3481 cggcccccgg ctcgccgcct ttcaggaacg cccacgcgac gaacgggaga gtccaggcga
     3541 cccagagggc gaggagcctc gtcgccgcca gcagcgtgtc gccccagggc cccgcggcgc
     3601 tcgagaagag ccccccttcc gtgtccccgg cgactccgac ctgcgcgaaa cgcgagacga
     3661 agacgaggac ggtccccatc cagacgacga ggacggcggc cgcgtccccc ttcgagcggg
     3721 ggccgctcgc cgcgaagagg aggacgagcc ccgtcgggag cgcggcgagg agccccgtga
     3781 agaaggaggt gaccgagaag gaatcgtgaa cgatctctcc gaagacgcgc gggaacgccg
     3841 tgcggctgga catgagacct cctgcctcga tccccggagc ccgggaacgg tccggatcga
     3901 tgcaccgatt ctagggggac ggacgccccg ggccatctgc tataaagccg gctttcgtag
     3961 gatatccatc gaaggaacct cacgaaggag acacgcgatg aagctccgtc cgacccttct
     4021 cctcgccgcc gccgcgctcg ccacgccggc gttcgcccag ttcgagaccc cgcagccgag
     4081 cccgaaggcg accctgagcc agcggatcgg cctgaccgac gtgacgatct cctacagccg
     4141 cccgagcgtc cgcggccggg ccatctgggg cgacctcgtc ccgtacgaca cgccgtggcg
     4201 caccggcgcg aacgccgcga ccacgctcac cttctccgac gacgtgacga tcgaggggca
     4261 gaagctcgcc gcgggcacct actccctcgt gacgatcccc gggaaggacg actggacggt
     4321 cgtcttcaac accgacacga agctctggtg ggagacgcag tacgacgccg ccaaggacgc
     4381 cctccgcgtg aaggtgaagc ccgaagccgc gccgatgacc gagacgctcc acatcgggtt
     4441 cccctcgatc tccgcgacgg gcgccgtcct cgcgatcgag tgggagaagg tccgggtccc
     4501 ggtgaaggtc ggcgtggagg tcgacgggaa gctgaagaag gccctcggcg ccgccgaggc
     4561 gggcgcctgg cagtcgcccc tgatggcggc ccgctactac tgggagcagc gccaggaccg
     4621 cccggaggcc tggaagcagc tcgagaagtc gatctccgcc gccaagaact gggcgaacgt
     4681 ctcccggaag gcgcgcttcc tcgccgaaga ggggaagacg gccgaggccg tcaaggcggg
     4741 cgaggaggcc ctcgccctcg cgaaggccga cccggcgaag ccgaacaccg cggcgttcga
     4801 gaagtcgctc ggggagtgga aggcgaagaa gtagccctct ccacgcccct ccgggattcg
     4861 cgaacgcggg cctcacggcc cgcgttcgca tccggggcgg tcccgccccc atcggcgacc
     4921 gcccccggcc gggccggggc cgcgtcgaag tccgcgtccg gggcgccgcc gtagagaaca
     4981 cagagggaag acgtgaccca gaagagccag gcccccggaa cgatgccgaa ggcccaccgg
     5041 ctcgagcggg agcagctcct tcccgggact cccgcggagg tcttcccgtt cttcgccgac
     5101 gcccggaacc tcgaggcgct gaccccgtcg ttcctcggct tccggatcct cacgcccctg
     5161 ccgatcgaga tgcgggaagg gacgctcatc gactactcgc tctccctctt cggcgtcccc
     5221 gtgcgatgga agacccggat ctccgcctgg gagccgggcg tgcgcttcgt cgacgagcag
     5281 ctttccggtc cctacgccct ctggcgccac acgcactcct tcgagcgccg cggggatacg
     5341 acgctgatgc gggacgtcgt cgactatcgc gagccgttcg gcccgctcgg gacgatcgcg
     5401 caccacctct tcgtcgcgag gaccgtcgag cggatcttcg actatcgccg ggaggcggtc
     5461 gaccggctcc tccggagggc cgcgtgagcg cgccggccgc cgacctcgag ctcttctacg
     5521 acggcgcctg ccccctctgc gcccgcgagg cgcgggcgct ccggaggctc gaccggcgcg
     5581 ggcggctgac gctcaccgac ctcgccgccc cgggcttcga cgcgggagag accggcgtcc
     5641 cgatggagcg cctcctcgct cggatccacg ggcgcctccc ggacgggacg atcgtcgagg
     5701 gcgtcgaggt cttccggaaa gcctacgaag cggtcggatt ccggctcctc gtcgcgccga
     5761 cgcgcctgcc gggcctctcg tggatcctcg accgggctta tgacgtcttc gcgcggaacc
     5821 gcctccggct gaccggccgc tgcgaggacg acgcgtgcag acggggcgcg cctacggcgc
     5881 cggcgtgacc gcgccgacgt cgatcgacgg cgtccgccac gtgaccgggt cgacgacgat
     5941 cttcccctcg tagttcagga cgtcgagctt ctccgggacg accttcacga ggacgaggtt
     6001 cttcagcccc gggaaggact ggtcccagta ggcgcgcttc ctcttcagga tctccgcccg
     6061 atcgtcgacg aggaccgccc gcccgctgag ggagacgtag ccgctcgcct tcgcgtggtc
     6121 ggcgtagtag agcgtcacgc gcgggtccgc gcgcatctcg gcgaccttcc ggctccggtc
     6181 gttcgtcgcg aaccagaccg tcaggtcgtc ctccggcggg aaagggttca tcgtccggac
     6241 cgaggggcgc cccgccgcgt cgagagtgat caaggcgcag taggtctgcg aggtcatcac
     6301 cttccgggcg gcggcgacga gctcgtcgcg ggtcgccggg gtcgcgggct tcgcggcctt
     6361 ctcgtccgcg aaggaggcgc cgctggcaag gagggcggcc agggcgagga ttgggaggcg
     6421 gagcatccgc cgggccttcg aggccatcgc gagtctcctt ccgggacgga gccgctcggg
     6481 atcgccccgg ttcgttgagg gcggcccggg ctcatgaagc ccgggccgcg gggttcatgc
     6541 ggaagcggtc tcccgggacg ccccgccggg cgccccccgg accgcggaga agccgagcgc
     6601 cacggcgacg acgacggcgc acccgagcgc gttcagccag aggaacgaga cgtcggtgag
     6661 gagccaggcg ccgacgaccg cggcctcgcc cgcgagggcg ccccagaacg cggcgccccc
     6721 cttcactttc gggaggtaga aggccgtcag gaagacgccg aggatcgtcc cgtagaagaa
     6781 ggagccgagg atgttcaccg cctcgacgag cgtcccgaga cggcccgcgt actgggcgaa
     6841 gacgatcgcg aacgctcccc aggcgaccgt gagccacttc gagacggaga cgagcttcgc
     6901 gtccgacgcc tccttcccga ggtaccggcg atagacgtcg acgacggagg tcgtcgagag
     6961 ggcgttcagc tcggccgagg tcgacgacat cgacgccgcg aagatggcgg ccaggacgag
     7021 gccgacgacg cccgtcggga ggacctccag gacgaatcgg aggaagacgt agttcgtgtc
     7081 cttcccctcc cctttcggga ccgcctcctt caccagggcc gccgccttcg ccttcacggc
     7141 ctcgctcttc ccgtgcgccg cgcgaagcga ctccttcgcc gccgcgagct gcgcctcgtc
     7201 gcccgatctc cccgccgcga cgaggcgcag cgcctcgacc cggcgctcct cgaacgcggc
     7261 cgcgtgggcc gtctcgagag cggccatctc ggccccgcgc gcctcggcgg ccttccgaga
     7321 ctcgaccggg ttgaaccaga gcggcggggc gacgaactgg tagaaggcga agaccatcgc
     7381 gccgacgaag aggatcgcga actgcatcgg caccttcacg aggccgttcg cgaggaggcc
     7441 gagccggctc tgggccacgg agcgccccgt gaggtagcgc tgcacctgcg actggtcggt
     7501 gccgaagtag gagagcgcga ggaacgcgcc cccgatcagc cccgaccaga ggttgtagcg
     7561 gtctccgagg tcgaacgtcg tctcgacggc gttcatccgt ccgagcgccc ccgacaccag
     7621 gaccgcgtcg ccgaagccga ccccctcggg gaggacccgg aggagggccg cgagggcgaa
     7681 gcccattgcg aggaggatga ccaccatctg ccacgtctgc gtctcgttga ccgccttcgc
     7741 gccgccggtc gccgtgtaga cgacgacgag gctcccgatc acgagcgtcg tcgcctggat
     7801 gctccagccg aggaggaccg agaggacgag cgccggggcg tagatcgtca ggccgcaggc
     7861 gaggccccgc tggaggagga agagcgacgc cgtgagcgtc cgcgtcttcg cgtcgaagcg
     7921 cccctcgagg tactcgtagg ccgtgtagac cttcagccgg tggaagagcg ggacgacggt
     7981 gacgcagagg atcaccatcg cgatcggcag cccgaagtag aactggacga agcgcatccc
     8041 gtcggcgaag gcctggccgg gcgtcgagag gaacgtgatc gcgctcgcct gcgtcgccat
     8101 gatcgagagg gcgacggtcc accaggcctg gctccgcccg gcgaggaggt acccctcgag
     8161 ccccttctgc ttccggctcc gccacgtccc gtagccgacg atgaagagga gcgacgcgcc
     8221 gaggacggcc cagtcgaggc ccttcaccgg aacgactccg cgaagagcca gaagaggagg
     8281 acctccagcg cgagcgcccc gaggacgacc gcgtagagga cgccccagcg cttcccgacc
     8341 ggcggcggct cgtcggcgac gggccctccg tcagccacgc ggcgtcccgc ccgagacgag
     8401 gttcgcgaag aggcggatcg cgcccggcac gccggccgga agctgccgga agaacgagag
     8461 ccccgtgtag acgtacgtcc ccttgccgtg cctcgcggcg aggagcgcgc ccttcgattc
     8521 cttctccccc gggtcggcca tcccgaggag aggcacgtag cgctcgtccc aggtcgccgg
     8581 gaagtagagg ccgcgctcct ggacccagcc ttcgaagtcg gcggggccga tccggttcgg
     8641 ggtcgtcagg agcggatgct ccggcgcgag gagcgtcacc ttcgactcct cgtccgtgac
     8701 tcggtcccgg cccagctcga gcgggaacgg gccgagccgg tcggtcaccc gctcgaagct
     8761 cgtgttgtac tggaggacga gcgtcccgcc cgcctccacc cagtcgaaga gccgcggcat
     8821 cgcgtccttc agctcggcgc gcgtgttcgc ggcgcggatg ccgaggacga cggcgtcgaa
     8881 gcgggcgagg tctccgcgaa ggaggtcgtc ggccgacagg agcgtgacgg cgtagcccat
     8941 ctggcggagg acgccgggga cctcgtcacc gggcccggag acgtagccga cgcgcgaccg
     9001 gccgcgcgcg aggtcgacgt ggaccgctcg cgcctcggcg cgctcgaaat acgtcagcgg
     9061 cggcaggtgc gggtactcga tccggcgctt cgtcaggccc caggaatcgc cccccgtcgc
     9121 cgcctccgcg gcgatcgtcg cgacggcggg cgtctccggg gagcggagcg tgaactcgaa
     9181 cgtcgcgctc gcgcccggcg cgagggcgaa cggccgcacg gcaggctcgg cccgccaccc
     9241 cgccgggacc gagagacgga cggcgccgtc gaccttcggc ggcggcgcct cgcccgccgg
     9301 gccatccgct ccggtggcgg acgcgacgat cgacggcgtg agcgggggcg gcccggagcg
     9361 gtccttcgga agctcggcgg cccggaccgt caccttcacg acccgaggcg cgtggtccgg
     9421 aaggacgagg acctcctcct cgatctcgac ggagacggga ggctcgacga cgaacgggcg
     9481 gatctcctcc cccttcaccg ggtcggtcct ccggaagtag accggctcct cgtacgtcag
     9541 cgtctccccc gccatgacga cgtcgaagga gacgcgaagg gaggcgccgt ccgggcggat
     9601 cgcgtccctc ggcgcgggaa cggcgtagag gccgcgtgaa gggcgcgccc ggagccagtg
     9661 cggctgcgtc gcctccgcgt cgaccggaac ggggacggac gccgagcccg acacgggttc
     9721 gttcagcttc agctcggcgc cggaggggag cccttctccc gtgccccagg ggacgacgac
     9781 gcgggcgagg gtcgccgggg tgccggcccg gacgagggcc gtgacgttca ggcggaaggc
     9841 gctccccggc gtcgccgagg ggctcgccgc gatcgcctcg agccagaggc cggaggccga
     9901 tcggatcgcc gcgaggagct cgcgacgctt gccctcgatc cacgggtcgg cgccgaggcc
     9961 gcgcagcgcg gcgtgcgcct cgaggagctt cgggaggacc cgcgagggct cccgggcgtc
    10021 gaagagccgc tcggcttccg ccagaagccg gccgacgtgc gcgccacccg gcacgcgcgc
    10081 ccacgtcgtg tcgatcccct cgaacgggtc ggccgtcacc gcgtctccct cgaggaggat
    10141 cagggcgttc gcgagccgcc cgcgccgctc cgacgagccg aagccctggc tcttgtgctg
    10201 gctccggctc tcggccgcgg tctccgtgtg cgagcgcccg agcgcggggc tgtagtcgcc
    10261 gatctcgtac gggatcgacc cctcgatcgg cttgccgtcc cgcctccagc cgttccagaa
    10321 gaggcgccgg ggctgccacg gggagagccc ttcctcgatc tgctccggga atcgcgtcgc
    10381 gtcgccggcg gcgcggaacg cctcgcgtgc aaggacggcc gaggccgtgt ggtgtccgtg
    10441 gcctccgtcg ccgttctcgg ggaagcgcgc gaggacgacg tcggggcgga acgtgcggat
    10501 cgcgcgcacg acgtcgcgga ggacctcctc gcgtccccag acgcggaacg tctcctcggc
    10561 cgtcttcgag tatccgaagt cgatcgcccg cgtgaacatc tgctcgccgc cgtcgatccg
    10621 ccgcgccgcg aggagctcgt gcgtcctcac gaggccgagc gccgggccga gctcggagcc
    10681 gatcaggttc tggccgccgt cgccgcgcgt gacggagagg taggccgtcc gcatcaggcg
    10741 cccttccgcg aacgccgcga tcatcgccgt gttctcgtcg tcggggtgcg ccgcgacgta
    10801 gaggacgctc ccgaggacgt tcagcttctt cagcttcagg aggagctctc ccgcgggggc
    10861 gggccgctcg ggcccgaagg gcgcggcggc cgccgcgggg gcggcggcga gggcgagaaa
    10921 ggcgacggcg agcggcgcgc cgaggcgcgc ggagcgtctg gtcatgcggt tctccggcgc
    10981 gcgattagac ctgcggcacg ctcccctgtc tacgcggagg gcgcccgcag ggtttcccgc
    11041 gcgttcaggt cctcttcccc gtcaggatga cgacggagcg cggccccacc tcgacccgga
    11101 gcgggtcggc tacgagcggc tcggcgccct gctcgaagac gtccgccggc ggctcgcggt
    11161 acgtgtccgc gacgacgtgc cagcgccgcc cttcggaggc gggagggagc tcgatccggt
    11221 gcgcctcgtg atgcgcgttc gcgacgacga agacgtcgtc cccctgcgcg gggtccttca
    11281 gctgcatgcc gagacaggtc gcgcccggcg accagtcggg gcggaacggg acgtgcccgt
    11341 gccaggtgac gggcgccgag cccgggcctt ccgggacgaa gctccggcgc cggagcagcg
    11401 ggcttcccct ccggaagcgg atcagcgtcc tcacgaacct cacgaggtcc gcgttctcct
    11461 cgacgaggcg ccagtcgagc caggagacct cgttgtcctg gcagtaggcg ttgttgttgc
    11521 cgccctgcgt ccggccggcc tcgtccccgg cgaggatcat cgggaccccc tgcgagacga
    11581 ggaggagcgc gaacgcgttc cgggccatcc tccggcggag ccgctccact tcgggcggcc
    11641 cgcccggtcc ttcgtggccg cagttccagg agttgccgtc gtcgccgccg tcgcggttcc
    11701 cttcgccgtt cgcctcgttg tacttgtgcg agtagcggaa gaggtcggcc agcgggaagc
    11761 cgtcgtggct cgtgacgaag ttgatcgagt ggtgcggggc ccgcccgccc ccctggtaga
    11821 ggtccgcgct tcccgacagc cgcgtcgcca gcagcgggac catcccgccg tctccgcgca
    11881 ggaaccggcg gaggtcgtcg cgatagcggc cgttccactc cgcccagcgc ccccagttcg
    11941 ggaagtgccc gacctggtag agcccggcgg cgtcccacgc ctctgcgatc agcttcgtgt
    12001 tcgcgaggac cgggtcggcc gcgatccgct cgagcagcgg cgggttcgtc aggaccgatc
    12061 cgtcgcgccc ccgcccgagg atcgaggcga ggtcgaagcg gaaaccgtcg acgtgcatct
    12121 ccgtgaccca ccaggcgagc gagtcgagga tcaggtccct cacgaccggg tggttgcagt
    12181 tgaaggtgtt cccggtcccc gagaagttcg cgtagcgccc ttcggcgtcc aggatgtagt
    12241 aggtcgagtt gtcgaggccg cggaacgacc aggtcggccc gcgttcgtcc ccctcgccgg
    12301 tgtggttgaa gaccatgtcg aggatcacct cgatccccgc ctcgtggagc gccttcacca
    12361 tctcccggaa ctcgtcgact gcggcgcccg gaccggcgct cgcggcgtag gcggagcggg
    12421 gcgcgaagaa ggagatcggg tggtagcccc agaggttctt caggcgctcg cccgtgaagg
    12481 ggttccggcg gtcgttgtcg ttctcctcga actccgtcac cggcatcagc tcgacggcgg
    12541 tgaccccgag gtcgacgagg tacgggacct tctccacgag gccgcggaac gtgccgggat
    12601 gcgcgacgcc ggaggaggag tgccgcgtga agctccggac gtgcagctcg tagatcaccg
    12661 agtcggccag gtgccggtcg atcggctggt cgaaacccca gtcgaactcg cggctcagga
    12721 tgacgccgcg gcgcgcgcgg acggactccg agcccttctc ctcgccgggc cgcggcggct
    12781 cgccccacgc ctcgcctccg gcgatggcgc gggcgtacgg gtcgaggagg agctcggggc
    12841 cgtagcggtg ccacggcgcg ccgggagcag tcgcccgctc cgcgcgccac gcccagcaca
    12901 ccgacgggtc cagcccgcga aggaacgcgt gccacacgtc gcccgtccgg ttgtagcgcg
    12961 ggtcgagcgg gaactccgcc gccggggtcg cgtcccccgg ggcgaagagg gcgagcgtga
    13021 ccgacgtggc gtgccgcgcg aagaccgaga agttcacccc gcgccacgag ggggtcgcgc
    13081 cgaaggggat cgggtgtccc cggaggacgg cgaagtcggc ggagatgtag tcgaggaatc
    13141 gccccgaggg gctggacgag ctcatgaaat tcgtacgcct tccggcgttc cgagcatacc
    13201 cacctcccgg ggtgccgtag actcctttca cccacgggga cgcggctcgg tccctcggtc
    13261 gggaatcgcc cccgcaggag gacagatgaa gcagaagacc ctcaccgcca ccctcgtcct
    13321 ggcggcgttc gtttccttcc cggtcgccgc gaccgggcag aagagcgaag cccccgggcc
    13381 cgccccggcc gccgaggccg ccatgaagat ccacgtcgat cccgccactg gccaggtcgt
    13441 cgccccgaag gccgacccgc aggccgccgg cccgcggacg acggccgcgg ccgtcgaagc
    13501 gttccccccg acccgcgtcg agaaggtgac gacgaaggcg ggcgggaaga aggtcgccct
    13561 ccacggccga ttcatgatgg agatgaccgc gaaggtcggc gccgacggga aggtctctca
    13621 cggctgcgaa gccgtcgagg agaccggcgc gaagcccgcc cctgcgaagg agcaccgtca
    13681 tgaccgctag ggcgctcctc ggcgggacgc tcgccgccgc tctcgcgctc gcggcccccg
    13741 ccgcggcgac gacgatcacc atcatcaacg tcgactcttc gggcgtcggg ttcaacgacc
    13801 cggccccgcg gaccccggtc ggcggcaaca acgggacgac gctcggcgag cagcgcctga
    13861 acgtcttcaa gtacgccgcc gccttctggg ccgcgcgggt cgactctccc gtcgagatca
    13921 ccgtcgaggc gaagttcgag gcgaaggcgt gcgacgccag cacggcgacc ctggggtcgg
    13981 ccggcacgcg ctacatcttc tccgacttca agggggcgcc ccgcgcgggc acgtggtact
    14041 cgagcgccct cgcgaacaag ctcgcgggcg aggacctcga gcccgccgaa gccgacatcg
    14101 cggcgagctt cacgaccgcc ctcgacgacg ggagctgcct cttcccgaag aagtggtact
    14161 acggcctcga cgcgcagccg acgggcagca ccaccgactt cgcgaccgtc gtcatccacg
    14221 agctcgggca cggcctcggc ttccagacgt tcgtcagccg gacgaccggc aagaagttct
    14281 ccggcgacga caaccccggc ctcgacgacg cctacatggt ccacctctac gacgggtcga
    14341 ccggcaagac gtgggaccag atgacggacg acgagcgcct cgcctccggg acgaacaccg
    14401 gcaacctcct ctggaacggc tcgaacgtcg tcgccgtcgc ctcgggcgtc ctgatccagg
    14461 gggtcggcgc cggcggccgg ccgctcatgt atgccccgaa cccgtcccag gccggctctt
    14521 ccgtctctca ctgggacacg acgctcacgc cgaacgagat gatggagccg tacgcgaccc
    14581 aggcgatgca ccggctcgac atcaccgacg agctgatgct ggacctcggc tggggcctcg
    14641 cctccgggag cacggaatac cactggatcc tcccctcctc cgcgaaggcc ccgggcaagg
    14701 gcggcgccta ttacacgacg gccctctcga tcggcaaccg cggcgccgcg gaggcgcgct
    14761 accggatgaa gttcctcggg cacgacgccg acggcaccgg cggcgccgag tcgtccgagt
    14821 tcgtcctcgg gccgaaccag tccgtcacct acgaggacgt cctcggctcg gtcttcggcc
    14881 tcgcgaacgg caactacggc gcgatccgcg tcacggcgaa cgtcccgtcg ctgaacgtcc
    14941 tcgggcagac ggcgacgccc gtcctcaccg actggaagag ctgcgtctcc tcgggcttct
    15001 tcggtcagtc ggtcccggcc gtgcccgcct ccggcctcat caccgccggc acgacgcggt
    15061 ccatcgtcgg cgtccgcgac gacgccgcct tccgaacgaa cctgatcctc gcgaacgccg
    15121 gcacgtcgat cgtgacggtg gacctgaccc tcctctcgtc ctccggcgcg atcctcggga
    15181 ctcgcaccgt ctcgcttcac ccgctcggca tgacccagat cggcccgctc gtcggaggga
    15241 tgggcgtcac cggggccgtc cgcgacgcgc agctcctcct gacgaccacg acgaccggcg
    15301 gggcattcgc cgcctacgcg agcgtcatcg acaacgtcac gaacgacccg aggacgctcc
    15361 tccccaactg acgcgccctt ctcgccggcg cgcctcgcgc ctcttcgagc cccggcccgc
    15421 cacggcggac cggggctctt cctcttccgg cggccgcgct tcagcgcggc gccggcagct
    15481 cgaagagctc gatcgagtcg cccgcgcggg cgaccgcgcg cgcgccgcgc accttcagct
    15541 ccgcgaggac gcgcgccgcc cggggccgcc cgtcgacgaa cgggaccgtc tccggcccga
    15601 cgaggaggag ctgccgggag acggcgacgt accgcgggag cggcgcgtcg gcggcgagga
    15661 ggtcgacgca gcccgggacg cggaacgacg gcaggtcgcc gccgaagtag gcgacgccga
    15721 ggtcgcccgg cgcgatcccg cgcgaggcgg agagcgcggc gagccgcggc aggtcctgac
    15781 cccagtcgag gttcgagtcc gcgagcggcg gctccggaaa gaggcgggcg gtgatcgagt
    15841 gccggccgac cgagcggccg atcgcgagcg gggagaggac gaggaggagg acgacggcgt
    15901 atccggcggg cgagcgtcga cgcagcggct ccccgagcgc cgcggcagcc ccggccgaga
    15961 ggaggacgat cacgggggcg aggtgcctca cgccgatctt cggcccggcg agggaggccg
    16021 cgaggaagac gccggccgag aagaggagga gccgcgcccg gagcggggca cggacgagaa
    16081 ccgcgaggag ccccgcgacg acgacgagga gccacggaag cggcgacttc acgaggagcg
    16141 ccacgaggtg atacgcgggg ccggggtcgg gggagacgtc gccgaggaag tacgagagcc
    16201 gccccgcccc ggtctgccgg aggacgaaga gggtcgagag ggcgtagcgc cccagcccct
    16261 cgtcgagccc cgcgatccgg gtgacgaggt cggcgtccgc cgccgggagc tggtagagcc
    16321 ggaggagcgt cgggaggacc gccgcctcgg tcgccgcgaa gcggccgagg atcagggaga
    16381 ggagggcaat cgtgaggacg gggaggccga gcgccacgag gacgaaccgg cgcgtcaccg
    16441 tccggtcgcg cgacatccgg agcgccggca cgacgaggag cggcaggagc ggaaggagga
    16501 cgagcgccgt ctgcttcgtc gcgatcgccg ccgcggccgc gagggcggtc ccgaggagcc
    16561 ggagcgcggt cggcttctcg tagagcgcga gggcgcccca gaccgccgcg acgacgagcg
    16621 ccgcggccgg gacgtcggcc gtgacgaggt ggccgtgcgg gaggagcgcc gtctgccccg
    16681 cgagaagcgc ggcagcgagg aggcccgcct ccccgcctcc ggcggcgcgc gaggcgaaga
    16741 ggaggaggag gacgaggaac gccacggtga cgaggcgcgc ggcgaagagg actttcaggg
    16801 ggtccgcggc gccgaagagc tgcgcgcgcg cggagaggaa gagcgtccgg aacgagaggg
    16861 ggggcgacga caccgcgccc gcgtgaaggg ccgcccgacc ctggagctcc ttggcgagcg
    16921 gagggagctc gacgttcacc gcccagctgc cgtgccgcgc ctggaggacg ccggcggcga
    16981 cgtggatcgg ctcgtccgcc gtgacgccgt cgcggcgcgc gtcgaggacg gcgaggacgg
    17041 cggtgaggag gagaagaccg ggaaggccga agaggacgag gcgggaaggg cgggtcacgc
    17101 ccctattgtg cgtccggcgg gacccccggc tcgcgcgacg gagccggccc gacgagccag
    17161 agggccgccg ccctcgccac gacc
//
LOCUS       DAOTEZ010000033        17067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2278_length_17067_cov_6.435986, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000033 DAOTEZ010000000
VERSION     DAOTEZ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17067
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2278_length_17067_cov_6.435986"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="P5164_02935"
     CDS             complement(<1..420)
                     /locus_tag="P5164_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42880.1"
                     /translation="MICQVCGASNELDAELCRKCQNKLLVVSGSSESYDEGPSGEGIS
                     LDEHLLERVSVLEEIVKRSAETLKALLEGFHRQEKNGFVVQTGLLALKDLLERKGLLV
                     EQEFVDLWEERVDQHMTVLEKRERFLERKERILAEHKG"
     gene            complement(417..1295)
                     /locus_tag="P5164_02940"
     CDS             complement(417..1295)
                     /locus_tag="P5164_02940"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003334206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HRY42881.1"
                     /translation="MAIERIGVIGAGQMGAGIAHVAALAGKAVVLTDVDAALAEKGLA
                     VVRKNLGRQVEKGKVAAEAADAALARISVASDLAALADADLVVEAIVENEEVKRGLFS
                     RLDGLLKSGAILASNTSSISITRLAAATKRPESVIGMHFMNPVPVMQLVEVIRGIATS
                     DATTEAVVAAAKEMGKVPVECRDFPGFVSNRVLMPMINEAVFAVFEGVATPEAVDTIM
                     KLGMNHPMGPLTLADFIGLDTCLAILNVLHRGLGDDKYRPCPLLVQMVDAGWVGRKAG
                     RGFYRYSGPGGEKVAG"
     gene            complement(1364..2257)
                     /locus_tag="P5164_02945"
     CDS             complement(1364..2257)
                     /locus_tag="P5164_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRY42882.1"
                     /translation="MARSRSFLFLTLLLASATGAAAEPVFVPAGEIRSGDLVALGREA
                     RVEGVLRGTIVTIGADVRIAGRVEKDVVALGGDVHLGPGSAVGGDVLALGGEVQASGP
                     GPAPVGGRLLTVGALEAAFAAELKTSPLAVRPVRGLFLAFRLALLLAWLIVGLVLLRL
                     APRPLTGASALVPGRLTAVTAIGAAAVLSALLVSALLLLVLPATAGLFAVGLLVSALA
                     LAKAFGIAAVFVALGRSLTRTAARGSLLFGDPAALAVGLALLGGVSLVPVVGPLAWSI
                     ASLLGIGTALAAAVRPTSAAS"
     gene            complement(2271..2780)
                     /locus_tag="P5164_02950"
     CDS             complement(2271..2780)
                     /locus_tag="P5164_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42883.1"
                     /translation="MTMHARTSDGNPKPGELELDRRMEVFLDEMVSSSVREELPADFA
                     SRVVAARPFAPWEVARPSFWRLPVAVGLGLLAGSLGLALTPLWSLGPATAVTVWAELL
                     AVAFGSPVATLVAALPLLAEGTERAAGAVSPGAVALLGGGAALVAGSLLGALARFRRP
                     ELPVARPRS"
     gene            complement(2794..3375)
                     /locus_tag="P5164_02955"
     CDS             complement(2794..3375)
                     /locus_tag="P5164_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRY42884.1"
                     /translation="MDAPEAPTDADLVARILGGEEELFTLLVDRYKLRLFRFVSRYLG
                     DPEDARDVTQEVFLKLYSALDSFDPRYRFSTWAFRIAGNAAIDHLRRRRIRAIPLELP
                     SDDESDRRSIDPVDGKPDPFEELSRTRLREALDLAIEKLPDDYRELISLRHHGELAYE
                     EIAELKGMPLGTVKNKLFRARQALRDLLPKGAV"
     gene            3495..4628
                     /locus_tag="P5164_02960"
     CDS             3495..4628
                     /locus_tag="P5164_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42885.1"
                     /translation="MRHHTTLPILLLALSTAALPAAGQPLPNATVATFPANPAPGDEI
                     VLKLSGQWPDGCVPETGRTKLTTSGTKLHVNFNYSGIPGPCTAAVTPWTLDVPVGPLA
                     AGTYTVEVTRTRTFSAPEAVGSGTFSVLPLAAATVYVPGFFAPGATVSMASTLAAYNN
                     TDKNVTVAFTGAWDGLGAAQPPADAAVGPNGAAVLDSHTLRAGQHVQMLAITAPRRVA
                     LRATLERLETVPEGLAKVPESLGRLELPVFAELFPAGMTAVAGDVSLSASECAEGATN
                     RRRVNLTLFNAGDAAATFRVSAVSATAGPGGPAAEKEYAVPASSIVQFNDLPLEGLPV
                     CAAGGAWFRITGDQPFLAYLSTVRPETFPGVLPYEIFPAKLDR"
     gene            complement(4719..6773)
                     /locus_tag="P5164_02965"
     CDS             complement(4719..6773)
                     /locus_tag="P5164_02965"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HRY42886.1"
                     /translation="MRSMSALAIPVLTLSFTAAAAGPAVPSAPAGPKPVDPASFDPAV
                     KPCEDFYQHAVGGWLKANPVPADKSRWGSFEELADRNRAVLKTILEETSAKADWPKGS
                     VKQKVGDFFASGMDEAAIETAGTKPLEPWLAKIDALKSSSDLPKLVADLHGRRLMAGY
                     AFFVGQDQKETTRYLAMLSQGGLGLPDRDYYVKDDAKSKELREKYAAHVARMLELLGD
                     APDAAKAKAATVMALETKLATAARTRVELRDPERNYNRKSVAELVAEAPGYDWAAYFE
                     ALGIAGLKELNVRQPEFAKAFAALAASEPLDAWKAYLRWHLVRASADVLPTRFEEEAF
                     SFYGRTLNGVPQQEERWKRVQAATDRALGEALGQLYVERAFSPKSKERMKVLVENLRA
                     SLKDRIAALPWMGDETKKAAQRKLAAFGVKIGYPDTWRDYSALEVSRGSYFENVVAAN
                     AFEAKRNLGKLGKPIDRTEWGMTPPTVNAYYSPTMNEIVFPAGILQPPFFWADGDDAV
                     NYGGIGVVIGHEMTHGFDDSGSRYDADGNLKNWWTEEDRKAYEARTALVVKQFDGYRP
                     LADQAINGKLTLGENVSDLGGLKVAYEALRKALGKDPNAAEKVEGYTPAQRFFLNYAS
                     VWRNNIRDEALRVRLNTDSHSPGRFRVLGPLSALPDFHAAFGCASGSPMVMPEAERPS
                     IF"
     gene            6872..7618
                     /locus_tag="P5164_02970"
     CDS             6872..7618
                     /locus_tag="P5164_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY42887.1"
                     /translation="MASGLPAFAFERATKRYGDLLALSELTLEVPPGVVFALVGPNGA
                     GKTTAIGLLTGLLAPTSGRAAVFGIDVAEDPVGAKRRLGFVPDRPYLWPRWTPRETLR
                     FVGAVFGARGAALEERIGIELSSFGLEEAADRTNETLSHGTRQKVALAQAFLHDPSLF
                     VLDEPMVGLDPLAQRGLVERLKERTAKGAAVLLTTHHLALAEEVADEAGVLVKGRLVA
                     RGAPLAMSARGSTGRLSELFFDLAAGAGEP"
     gene            7615..9261
                     /locus_tag="P5164_02975"
     CDS             7615..9261
                     /locus_tag="P5164_02975"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42888.1"
                     /translation="MSAGAVSLANSFRAAGRSLSPRSTAEAAGVLAVALFSGAFMAGE
                     YAGLAYLLRLGRSAMAQLPALVPEILLERLLLGAFAGAGILLLLGALTTAVSTLFLSE
                     ELLLRMALPIPHTRLLLRQVAATIALAAGPALLLALPAAVLAARSGAVGAMALAAAFL
                     AVAVLAGAAGSALALAVVAVFPPRRARLLAAFLSATGLAVAIVGARGARPERFLDPAA
                     ALDLLLRAGTTPLGVPGGNPVAFAARAASRALAGDATGLGQALLLLAGVLLLVVLLAR
                     LLAPVHLRLLRRSREDSDATPRRKALREASSAKHELLRAEVRTLLRDAATPAQLGTLA
                     AVFVLDLLNLKLLPGGDAASRDVVHGLQSGLGLFLVSALALRFAYPAVSTDGRSALLL
                     KSLPVSAARHLLVRWTVRAMPSLAAALLLAGASAWALEARGFPLGAALAVAAAGGLAI
                     PALHLGLGALLPRYDAPNPVSVALGPGGLVAMGLSTLLSLAPVLVVSEGLRSLVETLL
                     DTRLDGRALLAAWGATTSALSVAAMLAAARRLPKADVPAS"
     gene            complement(9263..10177)
                     /locus_tag="P5164_02980"
     CDS             complement(9263..10177)
                     /locus_tag="P5164_02980"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HRY42889.1"
                     /translation="MKVLVSQPLPEPSSTDLLSAFVVVIGRPDAWPEEARRHLPDADA
                     LVPTPREKVTGELLALAPHLKVVANCAVGTDNVDLEACRRRGIVVTNTPGVLTEATAD
                     LTWLLILAVARRFREGDALIRSGAWDGWKPMEMMGASLDGKTLGVFGPGRIGSAVARR
                     ASSFGMRVVTCDAADGPEAFERLLAESDVLTIHAPLTPATRGRFGEDELARMKPGAIL
                     VNTARGPIVDEAALASALASGRLGGAGLDVYEKEPAVHPGLLPLPNVVLLPHLGSATR
                     ETRLAMARTACDEAARVLRGEEPRHRVV"
     gene            10265..12694
                     /locus_tag="P5164_02985"
     CDS             10265..12694
                     /locus_tag="P5164_02985"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HRY42890.1"
                     /translation="MKASRALALGLIGAFALAGLLPQFEAAQPRGLSVTRPAAVAIAT
                     AEARRLGIPVGAAFRVETWEDSPLLEQELSGDAALRTRMERDPVVGPRLGGYRVTWFR
                     PGLEKFPEFGSVVVGVDGTVLGARARARAEEKGGKPTEAQVRSAAERFVASRAFPGAP
                     GPVLDSVRPTVLKDRADHLVRFRVPHDGPPASVSFFLNVYFVGEELAGWELFEEYGDG
                     RAFRYELGGTLTTTFVRFAVVFGILFLLLGVFLRKYHAGEVGVGTGAWLFAGQLVLFL
                     VLDVLTARADAYGFGFAGTDAFWTTVSQTAFRFLFLDVPLATLVFLSWSVGESYARER
                     WGERLASFDALLRRDARNATVGESLLTGLLLSPAVAAAALLPFVPALALGGVRPVLGD
                     GAYAVLVSEAGPATAVLGALSVAVPVAVVGGLLALSVFHRRHALWAGFLVSAVVGSIL
                     AVGLVPVGPETTRFLLGFGAPLAAAGVFLAKDLLASAVSFFGATLLLFLVPLSRAFEG
                     PALHGTLAALALPLLLLLALALAGLATGRRVEYRHEDLAPHVKRIVERERVKAEIDAA
                     NRIQAALLPPSDPKLPGVVVASHYRAATEIGGDYFDFLPLAGGRIGLAFGDVAGHGLT
                     SGIVMAMTKSALLVQTGHDPSPVKVMEVLNETVRKTAPKRMLMTFFFGVLDPATGNLR
                     YCSAGHLDPYVVRAGGERLDPLSAWGFPLGVRRREPFVEHVARLHPGDRLVLYSDGLI
                     EALDDDGEPFGFSRFEEVLTRAARDGAGEIRQALLTAVRRFTRNRPPEDDQTLVVLSL
                     EVARAAEEAVA"
     gene            complement(12714..13628)
                     /locus_tag="P5164_02990"
     CDS             complement(12714..13628)
                     /locus_tag="P5164_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HRY42891.1"
                     /translation="MPELPDVELYVEHLTRRLAGRTLERVRVASPFLLRTVEPSLADA
                     EGKTIRGVRRLGKRVVLELEGGPFLVVHLMVAGRFHWKDASRAPVPKKVGLAAFDVPG
                     ATLLLTEASPKKRAALHVVATESELAALSRGGLEPLAASLPEFAAALRCESHTMKRAL
                     TDPRLFSGIGNAYSDEILHAARMSPFALTRTMGDEEVARLHAATTATLSSWLARLRAE
                     AGERFPEKVTAFRPDFAVHGRYGKPCPVCGDPVQRLVYADNEANYCATCQTGGRLLAD
                     RALSRLLKEDWPRSLEGLEEAKAARRGP"
     gene            complement(13630..14532)
                     /locus_tag="P5164_02995"
     CDS             complement(13630..14532)
                     /locus_tag="P5164_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42892.1"
                     /translation="MIKKGCAIAAVVLLGVFGAYLYLLWGKVEAVPAIVLAGFGSLGL
                     LMVVGQLKAVLFGSGDVGALKRAEQGLPLEDGKEEAVWGRIEPLGAALAAPFSGRPCV
                     AYEYDAKKASSADDENATPSTLAGFALAPSVIRSSRGDVRLLGFSLLSDFPETVVDDP
                     EAPHRAVRHAASSQMEELGLAKLGALISAMDDAMADDDGSVRKDMKTKDATVADLEAC
                     VLTEKVVAPGETVTAIGIWDASRGGLVPKHRGKSAVVRLLPGGGAAMVVHGQKRPWGL
                     LFFSLLWAGFAHVFIWLAWKGGFE"
     gene            14657..15661
                     /locus_tag="P5164_03000"
     CDS             14657..15661
                     /locus_tag="P5164_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017973192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRY42893.1"
                     /translation="MEYRTLGRSGLKVSPLCLGTMMLGSWGNADEGECVRIVHRALDA
                     GINLVDTSNNYGEGTSEEILGKALGGRRNAAVLATKVWSRMGPGPNEQGLSRKAIQEQ
                     VEGSLRRLKTDVLDLYQIHRPDRTTPWDETLSTLTDLVRQGKVRYVGCSTSHATDPGV
                     SPWTVRLEAWEIVETLWISERNGFERWVSLQPPYSILRRTMEVDHFPVTERFGLGNLV
                     WSPLEGGWLTGKYRKGKPNPLDSPRAAKWVGDLGNPKFARRIEVVEELLALAEEKGTS
                     LARFALAWTLRHPAVTSTILGPRTPAQLDDALAALDVTVTEEDAARVDALVPPGTSAL
                     "
     gene            complement(15666..16694)
                     /locus_tag="P5164_03005"
     CDS             complement(15666..16694)
                     /locus_tag="P5164_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42894.1"
                     /translation="MAITKMFRDRVVSMEQTWDLGEKYLGLLSELVHEALPRFRTVGN
                     DFDHDRDFYFLLEQASEPDEERKVCFTRMLLADRDCIPLIADEPHAPVRERIVATIRA
                     QAENAEIVVAYRAILSEEEKAEADEIDAAWRIEEAAREAARKAEEERRREEKKRQRQA
                     EDTRRRAQREKQRARGGEAAGSAPARSGEAGEGRSRKRRRGRGGGGRADGSPQAEGTA
                     SGGGSAAPGASRPAGERPGGEPRRGDRPRGERPRGERPRGERPQSQKGAGATARAAEA
                     QAPGPAPQSPPRPAATGGAPDGGAPREGGGNRRRRRRGRGGSRPQGGGPNGGAPHGGG
                     APQGGPGE"
     gene            complement(16741..>17067)
                     /locus_tag="P5164_03010"
     CDS             complement(16741..>17067)
                     /locus_tag="P5164_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY42895.1"
                     /translation="FDACNAVVCPYRHATGSGIAGDALAHGRPVIGSDVPGLSVVVRH
                     GTSGLLVPPGDPRLLAAAMLRFVHEGLGPRLSAGAEAAGAQLSPEGHARTILALGGIG
                     PGPAGC"
BASE COUNT         2171 a         6505 c         5946 g         2445 t
ORIGIN      
        1 ccccttgtgc tcggcgagga tccgctcctt ccgctcgagg aagcgctcgc gcttctcgag
       61 gacggtcatg tgctggtcga cgcgctcctc ccagaggtcg acgaactcct gctcgacgag
      121 gagccccttc cgctcgagga ggtccttcag cgcgaggagg cccgtctgga cgacgaagcc
      181 gttcttctcc tggcggtgga acccctcgag gagcgccttc agcgtctccg cgctccgctt
      241 gacgatctcc tcgaggaccg agaccctctc gaggaggtgc tcgtcgaggg agatcccctc
      301 cccggacggc ccctcgtcgt acgactcgct cgagccggag acgacgagga gcttgttctg
      361 gcactttcgg cagagctcgg cgtccagctc gttcgacgcc ccgcagacct ggcagatcaa
      421 cccgcgacct tctccccgcc gggccccgaa tagcggtaga agccgcgccc ggcctttctg
      481 ccgacccagc cggcgtcgac catctggacg aggagcgggc agggacggta cttgtcgtcc
      541 ccgagccccc ggtgaaggac gttcaggatc gcgaggcagg tgtcgaggcc gatgaagtcc
      601 gcgagcgtga gcggccccat cgggtggttc atgccgagct tcatgatggt gtcgaccgcc
      661 tcgggcgtgg cgacgccttc gaagacggcg aagaccgcct cgttgatcat cggcatcagg
      721 acccggttcg agacgaagcc cgggaagtcg cggcactcga ccggcacctt ccccatctcc
      781 ttcgcggccg cgacgaccgc ctccgtcgtc gcatccgagg tcgcgatgcc gcggatcacc
      841 tcgacgagct gcatcaccgg gaccgggttc atgaagtgca tcccgatgac gctctcgggg
      901 cgcttcgtcg cggcggcgag ccgcgtgatc gagatcgaag aagtgttcga ggcgaggatc
      961 gcccccgact tcagaagccc gtcgagccgc gagaagaggc cgcgcttgac ctcctcgttc
     1021 tcgacgatcg cctccacgac gaggtcggcg tccgcgagcg cggcgaggtc gctcgcgacg
     1081 gagatccggg cgagagcggc atcggctgcc tccgcggcga ccttcccctt ctcgacctgg
     1141 cgtcccaggt tcttccggac gaccgcgagc cccttctcgg ccagggccgc gtcgacgtcg
     1201 gtaaggacca cggctttccc cgccagcgcc gccacgtggg cgatccccgc ccccatctga
     1261 cccgctccga tcaccccgat gcgctcgatc gccatcaccg ctcctccgcc ggtccgcgtc
     1321 ccgcgcgggt ccggccggcg gagcgtatca ggcgaaaatc gcctcaggag gccgcgctcg
     1381 tggggcgtac cgccgccgcc agggccgtcc cgatccccag aagcgacgcg atcgaccacg
     1441 cgagcggtcc gacgacggga acgagcgaca cgcccccgag cagggcgagc ccgactgcga
     1501 gggccgcagg atcgccgaag agcaggctcc ccctcgcggc cgtccgcgtc aggctcctcc
     1561 cgagggcgac gaagacggcg gcgatcccga aggccttcgc gagggcgagc gccgagacga
     1621 gcaggcccac ggcgaagagg ccggcggtcg caggaaggac gagaaggagg agcgcggaga
     1681 cgagaagggc ggagaggacg gccgcggcgc cgatcgccgt cacggcggtc agccggcccg
     1741 gcacgagcgc ggaggcgccg gtcagcgggc ggggagcgag ccgcaggagg acgaggccga
     1801 cgatcagcca ggcgaggaga agggcaagcc ggaacgcgag gaagaggccc cggacgggcc
     1861 gcacggcgag cggggacgtc ttcagctccg cggcgaaggc ggcctcgagc gctccgacgg
     1921 tcaggagccg gccaccgacg ggggccggac cggggccgct cgcctggacc tctcccccga
     1981 gggcgaggac gtctccgccg acggccgatc ccggaccgag gtgaacgtcc cctccgagcg
     2041 cgacgacgtc cttctcgacc cgtccggcga ttcggacgtc ggccccgatc gtcacgatcg
     2101 ttccccgcag cacgccctcg acgcgcgcct cccgccccag ggcgacgagg tcgcccgagc
     2161 gtatctctcc cgcggggacg aaaacgggct cagccgcagc gccggtcgcg ctcgcgagga
     2221 ggagcgtcag gaaaaggaag gagcggctcc gggccatctc cggccccggc tcagcttcga
     2281 gggcgggcga ccggaagctc cggccggcgg aagcgggcca gcgccccgag gagagagccg
     2341 gccacgagcg ccgccccacc tccgaggagc gccacggcgc cgggcgagac ggcccccgcg
     2401 gcccgctcgg tcccctccgc gaggagaggg agggcggcga cgagggtggc gaccgggctt
     2461 ccgaaggcga cggcgagaag ctccgcccag accgtcaccg cggtcgcggg gccgaggctc
     2521 cagagcgggg tcagggcgag cccgaggctc ccggccagga gtccgagacc gacggcgacc
     2581 gggagacgcc agaaggacgg ccgggcgacc tcccacgggg cgaacggcct cgccgccacg
     2641 acccgggagg cgaagtcggc cggcagctcc tcccgaaccg aagagctaac catctcgtcc
     2701 agaaacactt ccattcgccg atcgagctcg agctcgcctg gtttcgggtt cccgtcggag
     2761 gtcctcgcgt gcatcgtcat cgtcccgctc tctctatacg gcgcctttcg gcaaaagatc
     2821 tcgcagggcc tggcgggccc ggaagagctt gttcttgacc gtcccgaggg gcatcccctt
     2881 cagctcggcg atctcctcgt aggccagctc cccgtgatgg cggaggctga tcagctcccg
     2941 atagtcgtcc gggagcttct cgatcgcgag gtcgagcgct tcccggaggc gggtccggga
     3001 gagctcctcg aacgggtcgg gtttgccgtc gaccgggtcg atggaccggc ggtccgactc
     3061 gtcgtcggac gggagctcga ggggaatggc ccggatcctc ctccgacgca ggtggtcgat
     3121 cgcggcgttc ccggcgatcc ggaacgccca cgtcgagaac cgatagcgcg ggtcgaagga
     3181 gtcgagcgcg gaatagagct tgaggaagac ctcctgcgtc acgtcccgcg cgtcctcggg
     3241 gtcgccgagg tatcgggaga cgaaacggaa gagccggagc ttgtagcggt cgacgaggag
     3301 cgtgaagagc tcctcctcgc ccccgaggat ccgggcgacg aggtcggcgt cggtcggcgc
     3361 ttccggggcg tccaccccgc gatcctgcca caggcccgaa cgagggaact gacgcggcgc
     3421 gcacgaatcc cacgcgccgc gatcgcgcgt gggacggccg tgcgtcgcgg cactccggac
     3481 cggaaggtga ttgcatgaga caccacacga ccctcccgat cctcctcctc gccctctcca
     3541 ccgccgccct cccggccgcg ggccagccgc tcccgaacgc cacggtggcg acgttcccgg
     3601 ccaaccccgc gcccggcgac gagatcgtcc tgaagctctc cgggcagtgg cccgacggct
     3661 gcgtccccga gacgggccgg acgaagctga cgacgtccgg gacgaagctg cacgtcaact
     3721 tcaactactc cggcatcccc gggccgtgca ccgcggccgt caccccctgg acgctcgacg
     3781 ttccggtcgg cccgctcgcc gccgggacct acaccgtcga ggtcacccgg acccggacgt
     3841 tcagcgcccc cgaggcggtc ggcagcggca cgttctccgt cctcccgctc gccgccgcga
     3901 ccgtctacgt cccgggcttc ttcgctcccg gcgcgacggt ttcgatggcg agcaccctcg
     3961 ccgcctacaa caacaccgac aagaacgtca ccgtcgcgtt cacgggcgcc tgggacgggc
     4021 tcggcgccgc gcagccgccc gccgacgccg ccgtcggtcc gaacggcgcc gccgtcctcg
     4081 actcccacac gctccgcgcg ggacagcacg tccagatgct cgcgatcacg gcgcccaggc
     4141 gcgtcgcgct ccgcgccacg ctcgagcgcc tcgagacggt tcccgagggg ctcgcgaagg
     4201 tccccgagtc cctcggccgc ctcgagctcc ccgtcttcgc cgagctcttc ccggccggca
     4261 tgaccgccgt cgccggcgac gtctccctct ccgcctccga gtgcgccgaa ggcgcgacga
     4321 accgccgccg cgtgaacctg acgctcttca acgcaggcga cgcggccgcc acgttccgcg
     4381 tctcggccgt ctcggcgacg gccggccccg gcggcccggc cgcggagaag gagtacgctg
     4441 ttcccgcctc ctcgatcgtc cagttcaacg acctcccgct ggagggcctg ccggtctgcg
     4501 cggcgggggg agcgtggttc cggatcaccg gcgaccagcc gttcctggcg tatctctcga
     4561 ccgttcgccc ggagaccttc cccggggtcc tcccctacga gatcttcccc gcgaagctcg
     4621 atcgctgacc gtccctcccg cggcgcagcc gccgcgtctc ccacccccgc gggggccggc
     4681 accgccggcc cccgcttctc gtttcggggg gagcggacct agaagatgct cggccgctcc
     4741 gcttcgggca tcaccatcgg gcttccggac gcgcatccga aggcggcgtg gaaatccggg
     4801 agggccgaga gggggccgag gacgcggaaa cgtcccgggc tgtgcgagtc ggtgttgagc
     4861 cggacgcgga gcgcctcgtc gcggatgttg ttcctccaga ccgacgcgta gttcaggaag
     4921 aagcgctggg ccggggtgta cccctcgacc ttctccgccg cgttcgggtc cttgccgagc
     4981 gccttgcgga gggcctcgta ggcgactttc agcccgccga ggtccgagac gttctccccc
     5041 agcgtgagct tcccgttgat cgcctgatcc gcgaggggcc ggtacccgtc gaactgcttc
     5101 acgacgaggg ccgtcctcgc ctcgtaggcc ttgcggtcct cctcggtcca ccagttcttc
     5161 aggttcccgt cggcgtcgta gcggctcccg gagtcgtcga agccgtgcgt catctcgtgc
     5221 ccgatgacga cgccgatgcc gccgtagttc acggcgtcgt ccccgtcggc ccagaagaac
     5281 ggaggctgga ggatcccggc cgggaagacg atctcgttca tcgtcgggct gtagtaggcg
     5341 ttcaccgtcg gcggggtcat cccccactcg gtccggtcga tcggcttgcc gagcttgccg
     5401 aggttccgct tcgcctcgaa cgcgttcgcc gcgacgacgt tctcgaagta ggagccgcgg
     5461 gagacctcga gggccgagta gtcgcgccac gtgtcggggt agccgatctt tacgccgaaa
     5521 gccgcgagct tcctctgcgc cgccttcttc gtctcgtccc ccatccaggg gagggccgcg
     5581 atccggtcct tcagcgaggc gcgaaggttc tcgacgagga ccttcatccg ctccttcgac
     5641 ttcggcgaga acgcgcgctc gacgtagagc tggccgaggg cttcgccgag ggcgcggtcc
     5701 gtcgcggcct ggaccctctt ccagcgctcc tcctgctgcg gcacgccgtt cagcgtccgg
     5761 ccgtagaagg agaacgcctc ctcctcgaac ctcgtcggga ggacgtcggc cgaggcgcgg
     5821 acgaggtgcc agcggaggta ggccttccac gcgtcgagcg gctcggacgc ggcgagcgcc
     5881 gcgaacgcct tcgcgaactc gggctggcgg acgttgagct ccttcaggcc cgcgatcccg
     5941 agcgcctcga agtaggccgc ccagtcgtac ccgggggcct cggcgacgag ctccgcgacg
     6001 ctcttcctgt tgtagttccg ctccgggtcg cgaagctcga cccgcgtccg cgcggccgtc
     6061 gcgagcttcg tctcgagggc catcaccgtc gcggccttcg ccttcgccgc gtccggggcg
     6121 tcgccgagga gctcgagcat ccgcgccacg tgggcggcgt acttctcgcg gagctccttc
     6181 gatttcgcgt cgtccttcac gtagtagtcg cggtcgggga ggccgagacc gccctgggag
     6241 agcatcgcga ggtagcgggt cgtctccttc tggtcctgcc cgacgaagaa cgcgtagccc
     6301 gccatcagcc gccgcccgtg gaggtcggcg acgagcttcg ggaggtcgga gctcgacttc
     6361 agggcgtcga tcttcgcgag ccagggctcg agcggcttcg tcccggccgt ttcgatggcg
     6421 gcctcgtcca tgccggaggc gaagaagtcg ccgaccttct gcttcacgct ccccttcggc
     6481 cagtcggcct tcgccgaggt ttcctcgagg atcgtcttca ggacggcccg gttccggtcg
     6541 gcgagctcct cgaaagagcc ccagcgcgac ttgtcggccg ggaccgggtt cgccttcagc
     6601 cagcccccga cggcgtgctg gtagaagtct tcgcacggct tcacggccgg atcgaacgag
     6661 gccgggtcga ccggcttcgg gcctgccggg gcggacggaa cggccggacc ggccgcggcg
     6721 gccgtgaacg agagggtcag gacggggatc gccagggcgg acatcgagcg catcgctcct
     6781 ccttgtcgag ccgactgtac gacgtgcgcc ggagcggggt ttcgacgagg gagatcggcc
     6841 ggcgcccccg acctcccgta cgatcgggcc ggtggcttcc ggcctccccg ccttcgcctt
     6901 cgagcgcgcg acgaagcgct acggcgacct gctcgccctc tcggagctga cgctcgaggt
     6961 cccgccggga gtcgtcttcg cgctcgtcgg gccgaacgga gccgggaaga cgacggcgat
     7021 cggcctcctg accgggctcc tcgcaccgac ctccgggcgg gcggccgtct tcggcatcga
     7081 cgtggcggag gacccggtcg gggcgaagcg gcggctcgga ttcgtgccgg accgcccgta
     7141 cctctggccg cggtggacgc cgcgcgagac gctccgattc gtcggggcgg tcttcggggc
     7201 gcggggagcc gcgctcgagg agaggatcgg gatcgagctc tcctccttcg gcctcgagga
     7261 ggcggccgac cggacgaacg agacgctctc ccacggcacg cgacagaagg tcgccctcgc
     7321 gcaggccttc ctccacgacc cgtcgctctt cgtcctcgac gagccgatgg tcggcctcga
     7381 cccgctcgcc cagcggggcc tcgtcgaacg gctgaaggag cgaacggcga agggtgccgc
     7441 cgtcctcctg acgacgcacc acctcgccct cgccgaggag gtcgccgacg aggcgggcgt
     7501 cctcgtgaag gggaggctcg tcgcgcgcgg cgcgccgctc gcgatgagcg cgcgcggctc
     7561 gacggggcgg ctctcggagc tcttcttcga cctcgccgcg ggcgccgggg agccgtgagc
     7621 gccggggcgg tctccctcgc gaactcattc cgcgcggccg gccggtcgct ctcgccgcgc
     7681 tcgacggcgg aggccgcggg agtcctcgcg gttgcgctct tctcgggggc gttcatggcc
     7741 ggtgagtacg cgggcctcgc gtacctcctt cgcctcggac ggagcgccat ggcccagctc
     7801 cccgccctcg tcccggagat cctcctcgag cggctcctcc tcggagcgtt cgccggagcg
     7861 ggaatcctcc tcctcctcgg cgccctgacg acggccgtct cgacgctctt cctctccgag
     7921 gagctcctcc tccggatggc gctcccgatc ccgcacacgc gcctcctcct tcgccaggtc
     7981 gccgcgacga tcgcgctcgc cgccggcccc gcgctcctcc tcgccctccc cgcggccgtc
     8041 ctcgcggccc gctcgggcgc ggtcggcgcg atggcgctcg ccgccgcgtt cctcgccgtc
     8101 gccgtcctcg cgggagcagc cgggtcggcg ctcgcgctcg cggtcgtcgc cgtctttccc
     8161 ccgcggcgcg cccggctcct cgcagcgttc ctctcggcga cgggcctcgc cgtcgcgatc
     8221 gtcggagcac gcggggcgcg ccccgagcgc ttcctcgatc ccgccgccgc cctcgacctc
     8281 ctcctccgcg ccggcacgac gcccctcggc gtgccgggcg ggaaccccgt ggccttcgcc
     8341 gcgcgggccg cctcgcgcgc cctcgcgggc gacgcgacgg gcctcggcca ggcccttctc
     8401 ctcctcgccg gcgtgctcct cctcgtggtc ctcctcgcgc gcctcctcgc tccggtccac
     8461 ctccgactcc tccggcggag ccgcgaggac tcggacgcga cgccgcgccg gaaggccctc
     8521 cgggaggcgt cctccgcgaa gcacgagctc cttcgcgccg aggtccggac gctcctgcgc
     8581 gacgcggcga caccggcgca gctggggacg ctcgccgccg tcttcgtcct cgacctcctg
     8641 aacctgaagc tcctccccgg gggcgacgcc gcctcgcgcg acgtcgtcca cggcttgcag
     8701 tcgggcctgg ggctcttcct cgtctcggcg ctcgcactcc gattcgccta ccccgccgtc
     8761 tcgaccgacg gacgctcggc gctcctcctg aagtcgctcc ccgtctccgc agcgcggcac
     8821 ctcctcgtaa ggtggacggt ccgggcgatg ccgtccctcg cggcggcgct cctcctcgcg
     8881 ggcgcctccg cgtgggccct cgaggcgcgc ggcttcccgc tcggcgccgc gctcgccgtc
     8941 gccgcggcgg gcggcctcgc gatcccggcg ctccacctcg gcctcggcgc gctcctcccc
     9001 cgctacgacg ctccgaatcc cgtttccgtc gccctcggcc ccggcggcct cgtcgcgatg
     9061 gggctctcga cgctcctctc cctcgccccg gtcctcgtcg tgagcgaagg gctccggagc
     9121 ctcgtcgaga cgcttctcga cacgcgcctc gacggccgag ccctcctggc cgcgtggggt
     9181 gcgaccacgt cggcgctctc ggtggcggcg atgctcgccg ccgcacggcg cctcccgaag
     9241 gccgacgtcc cggcctcctg agctacacga cccggtgccg tggctcctcg ccgcgcagga
     9301 cgcgcgccgc ctcgtcgcag gccgtccgcg ccattgcgag gcgcgtctcg cgcgtcgccg
     9361 agccgaggtg cgggaggagg acgacgttcg ggagcgggag gaggccgggg tggaccgccg
     9421 gctccttctc gtagacgtcg aggccggcgc cgccgaggcg gcccgacgcg agcgccgacg
     9481 caagcgccgc ctcgtcgacg atcgggccgc gggcggtgtt cacgaggatc gctccgggct
     9541 tcatccgggc aagctcgtcc tccccgaagc ggccgcgcgt cgccggggtg agcggcgcgt
     9601 ggatcgtcag gacgtccgac tcggcgagga gccgctcgaa ggcttccggt ccgtcggcgg
     9661 catcgcaggt gacgacgcgc atcccgaacg acgaggcgcg tcgggcgacc gcgctcccga
     9721 tccgacccgg cccgaagacg ccgagggtct tcccgtcgag cgaggcgccc atcatctcca
     9781 tcggcttcca cccgtcccac gcccccgaac ggatcagggc gtctccttcg cggaagcgcc
     9841 gcgcgacggc gaggatcagg agccaggtca ggtcggcggt cgcctccgtc aggactcccg
     9901 gcgtgttcgt gacgacgatc ccgcgccggc ggcaggcctc gaggtcgacg ttgtcggtgc
     9961 cgacggcgca gttcgcgacg accttgaggt gcggggcgag ggcgaggagc tccccggtca
    10021 ccttctcgcg gggcgtcggg accagcgcgt cggcgtccgg gaggtgccgg cgcgcctcct
    10081 ccggccaggc gtccggccgg ccgatgacga cgacgaacgc cgacaggagg tcggtcgagg
    10141 acggctccgg aagcggctgg gagacgagaa ccttcacgac ggaaggttaa cgccgccagc
    10201 gtacgatccc cctgatgccg ccctcgcgga acctcccgga cgccccgttc gtaatgaggg
    10261 gggcatgaag gcctcgcgtg ccctcgccct cggcctgatc ggggccttcg ccctcgccgg
    10321 cctcctgccg cagttcgagg cggcgcagcc gcgcgggctt tccgtgaccc gccccgccgc
    10381 ggtcgcgatc gcgacggccg aggcgcgccg gctcgggata cctgtcggcg cggcgttccg
    10441 ggtcgagacg tgggaggact ctccgctcct cgagcaggag ctgtcgggcg acgcggcgct
    10501 ccgcacgcga atggagcggg acccggtcgt cggcccccgg ctcggcgggt accgcgtcac
    10561 gtggttccgg ccgggcctcg agaagttccc ggagttcggg tcggtcgtcg tcggcgtcga
    10621 cggaacggtc ctcggcgcgc gggcgcgggc ccgggccgag gagaaagggg gcaagccgac
    10681 ggaggcccag gtccggtccg cggccgagcg gttcgtcgcc tcgcgcgcct tccccggcgc
    10741 cccgggcccg gtcctcgact ccgtgcggcc gaccgtcctg aaggaccggg ccgaccacct
    10801 cgtccggttc cgcgtcccgc acgacgggcc cccggcgtcc gtctcgttct tcctgaacgt
    10861 ctacttcgtc ggcgaggagc tcgccggctg ggagctcttc gaggagtacg gagacggccg
    10921 cgccttccgc tacgagctcg gcgggacgct cacgacgacg ttcgtccggt tcgccgtcgt
    10981 tttcggcatc ctcttccttc tcctcggcgt cttcctccgg aagtaccacg cgggcgaggt
    11041 cggggtgggg acgggagcgt ggctcttcgc cggccagctc gtcctcttcc tcgtcctgga
    11101 cgtcctgacc gcgcgggccg acgcctacgg cttcggcttc gccgggacgg atgcgttctg
    11161 gacgaccgtc tcgcagaccg ctttccggtt cctcttcctc gacgtcccgc tcgcgacgct
    11221 cgtcttcctc tcctggtcgg tcggcgagag ctacgcgcgc gaacgatggg gagagcggct
    11281 cgcgtccttc gacgccctcc tccgacgcga cgcccggaac gccaccgtgg gcgaatcgct
    11341 cctgaccgga ctcctcctct cgcccgccgt cgcggccgcc gcgctcctgc cgttcgttcc
    11401 cgcgctcgcg ctcgggggcg tccgtcccgt cctcggagac ggcgcgtacg ccgtcctcgt
    11461 ctccgaggcc ggaccggcga ccgccgtcct cggcgccctc tccgtcgccg tcccggtcgc
    11521 cgtcgtcggc ggcctcctcg ccctctcggt cttccaccgc cgccacgcgc tctgggccgg
    11581 cttcctcgtc tcggccgtcg tcggctcgat cctcgccgtc ggcctcgtac cggtcggccc
    11641 cgagacgacc cgtttcctcc tcggcttcgg ggcgccgctc gccgcagccg gcgtcttcct
    11701 cgcgaaggac ctcctcgcct cggccgtgtc gttcttcggc gcgacgctcc tcctcttcct
    11761 cgtcccgctc tctcgcgcct tcgaggggcc ggcgctccac ggcactctcg ccgcgctcgc
    11821 ccttcccctc ctcctcctcc tggcgctcgc gctggccggc ctcgcgacgg gccggcgcgt
    11881 cgagtaccgc cacgaggacc tcgccccgca cgtgaagcgg atcgtcgagc gggagcgggt
    11941 gaaggccgag atcgacgccg cgaaccggat ccaggccgcc ctcctcccgc cgagcgaccc
    12001 gaagctcccg ggggtcgtcg tcgcctccca ctatcgcgcc gcgaccgaga tcgggggcga
    12061 ctacttcgac ttccttcccc tcgccggagg gcgcatcggc ctcgcgttcg gcgacgtcgc
    12121 cggacacggc ctgacgagcg gcatcgtcat ggcgatgacg aagtcggccc tcctcgtcca
    12181 gaccggccac gacccgtccc cggtgaaggt catggaggtc ctgaacgaga cggtgcggaa
    12241 gacggcgccg aagcggatgc tcatgacctt cttcttcggc gtcctcgacc cggcgacggg
    12301 aaacctccgt tactgctcgg cgggccacct cgacccgtac gtcgtgcgcg cgggcggcga
    12361 gcgcctcgac ccgctctcgg cctggggatt cccgctcggg gtcaggaggc gcgagccgtt
    12421 cgtcgagcac gtggcgcggc tccacccggg cgaccgcctc gtcctctaca gcgacggcct
    12481 catcgaggcg ctcgacgacg acggcgagcc gttcggcttc tcgcgcttcg aggaggtcct
    12541 cacccgcgcc gcgcgcgacg gcgccggcga gatccggcag gcgctcctga cggccgtacg
    12601 gcggttcacc cgcaaccgcc cgcccgagga cgaccagacg ctcgtcgtcc tctccctcga
    12661 ggtcgcccgc gccgctgaag aagcggtcgc ctgaacggcc cggacgttcg acctcagggc
    12721 ccccggcggg cggccttcgc ctcctcgagc ccctcgaggc tccgcggcca gtcctccttc
    12781 aggaggcgtg aaagggcgcg gtccgccagg agccgtcctc ccgtctggca ggtcgcgcag
    12841 tagttcgcct cgttgtcggc atagacgagc cgctggaccg ggtcgccgca gacggggcaa
    12901 ggcttcccgt agcggccgtg gacggcgaag tcgggccgga acgcggtcac cttctccgga
    12961 aagcgctccc ccgcctccgc gcggagacgc gcgagccacg acgagagcgt cgccgtcgtc
    13021 gcggcgtgga ggcgcgcgac ctcctcgtcg cccatcgtcc gcgtcagggc gaacggcgac
    13081 atccgcgccg cgtggaggat ctcgtcggag taggcgttgc cgatgccgga gaagaggcgc
    13141 gggtcggtca gcgcccgctt catcgtgtga ctctcgcagc ggagggccgc cgcgaactcc
    13201 ggaaggctcg ctgcgagcgg ctcgaggccg ccgcgcgaga gcgctgcgag ctccgactcc
    13261 gtcgcgacga cgtggagcgc cgcccgcttc ttcgggctcg cctccgtcag gaggagcgtg
    13321 gcgcccggga cgtcgaacgc ggcaaggccg actttcttcg ggacgggtgc tcgcgaggcg
    13381 tctttccaat ggaagcgccc ggcgaccatc aggtggacga cgaggaaggg ccccccctcg
    13441 agctcgagga cgacccgctt tccgagcctt cggacgccgc ggatcgtctt cccctcggcg
    13501 tcggcgagcg atggctcgac cgtccggagg aggaacggcg aggcgacgcg gacccgctcg
    13561 agcgtccggc cggcgagccg acgcgtcagg tgctcgacgt aaagctcgac gtcggggagc
    13621 tcgggcacgt cactcgaacc cgcccttcca ggcgagccag atgaagacgt gcgcgaagcc
    13681 ggcccagagg agggagaaga agaggagccc ccagggccgc ttctgcccgt ggacgaccat
    13741 cgcggcgccg ccgcccggaa ggaggcggac gacggccgac ttcccgcggt gcttcgggac
    13801 gaggccgccg cgcgaggcat cccagatccc gatcgcggtc acggtctctc ccggagcgac
    13861 gaccttctcc gtcaggacgc acgcttccag gtcggcgacc gtcgcgtcct tcgtcttcat
    13921 gtccttccgg accgagccgt cgtcgtcggc catggcgtcg tccatcgccg agatcagcgc
    13981 gccgagcttc gcgagcccca gctcctccat ctgcgaggag gcggcgtgcc ggacggcgcg
    14041 gtgcggcgcc tcggggtcgt cgacgacggt ctccgggaag tcggagagga ggctgaagcc
    14101 gaggagcctc acgtcgccgc gactcgagcg gatgacggac ggggccagcg cgaacccggc
    14161 gagcgtcgag ggtgtcgcgt tctcgtcgtc ggcggaggag gccttcttcg cgtcgtattc
    14221 gtaggccacg cacggccgcc cgctgaacgg cgccgcgagt gcggcgccga gaggctcgat
    14281 ccggccccag accgcctcct ccttcccgtc ctcgagcggg agcccctgct cggcccgctt
    14341 cagcgcgccg acgtcgccgg agccgaagag gacggccttc agctgcccga cgaccatgag
    14401 gaggccgagc gagccgaacc ccgcgaggac gatcgcgggt accgcctcga cttttcccca
    14461 gaggagatag aggtaggccc cgaaaacccc gagcaggacg acggcggcga tcgcgcagcc
    14521 cttcttgatc acgtctcctc ctcgagaggc cgcgcggcgc cggcccgctg ccccgttgca
    14581 cgacgatccg agagcttttc cgagtctacc gtccgggcgt aacgtctgca cgcggccccg
    14641 gccgcgggag gcccccatgg aataccggac tctgggacgc tcgggcctga aggtctcgcc
    14701 cctctgcctc ggcacgatga tgctcggaag ctgggggaac gccgacgagg gcgagtgcgt
    14761 gcggatcgtc caccgcgcgc tcgacgcggg gatcaacctc gtcgacacct cgaacaacta
    14821 cggcgagggg acgtcggagg agatcctcgg gaaggccctt ggcgggcggc ggaacgcggc
    14881 ggtcctcgcg acgaaggtct ggtcccggat ggggcccggc ccgaacgagc aggggctgtc
    14941 ccggaaggcg atccaggaac aggtcgaagg gagcctcagg cgcctgaaga ccgacgtcct
    15001 cgacctctac cagatccacc gccccgaccg gacaacgccg tgggacgaga cgctctcgac
    15061 gctgacggac ctcgtccgcc aggggaaggt ccgctacgtc ggctgctcga cgagccacgc
    15121 cacggacccc ggggtgagcc cgtggaccgt ccgcctcgag gcgtgggaga tcgtcgagac
    15181 gctctggatc tccgagcgaa acggcttcga gcgctgggtc tcgctccagc cgccctactc
    15241 gatcctccgg aggacgatgg aggtcgacca cttccccgtc accgagcgct tcggcctcgg
    15301 gaacctcgtc tggagcccgc tcgaaggggg ctggctcacc ggcaagtacc ggaaggggaa
    15361 gccgaatccg ctcgactctc cgcgcgccgc gaaatgggtc ggagacctcg ggaacccgaa
    15421 gttcgcgcga cggatcgagg tcgtcgagga gctcctcgcg ctcgctgagg agaaggggac
    15481 gagcctggcc cgcttcgccc tcgcctggac gctccgtcac cccgcggtga cctccacgat
    15541 cctcggcccg cgaacgcccg cgcagctcga cgacgcgctc gccgcgctcg acgtgacggt
    15601 cacggaggaa gacgccgccc gcgtcgacgc gctcgtccct cccgggacga gcgcactcta
    15661 gaccgctact ccccgggacc tccctgcgga gcgccgcctc cgtgcggagc gcctccgttc
    15721 ggcccgcccc cctgcggccg gctgccgccg cgcccgcgcc gacgcctcct gcggttcccg
    15781 ccgccttcgc gcggtgcgcc gccgtccggc gctccgcccg tcgcggccgg gcgaggcggc
    15841 gactgcgggg ccggaccggg agcctgcgcc tccgccgccc gagccgtcgc tccggcgccc
    15901 ttctgcgact gcggtcgctc gccgcgaggg cgttccccgc gcggcctttc gccgcgcgga
    15961 cggtccccgc gccgcggctc tccccccggc cgctcgccgg ccgggcgcga ggcgcccggg
    16021 gcggccgatc cgccgcccga ggccgtccct tccgcctgcg gcgagccatc cgcccgcccg
    16081 ccgccgcccc ggccgcgccg gcgcttccgc gagcgccctt ccccggcctc gccgctccgc
    16141 gccggtgcgg atccggcggc ctcgccgcca cgcgcccgct gcttctcgcg ctgcgcccgg
    16201 cgccgggtgt cctcggcctg ccgctgccgc ttcttctctt cgcggcggcg ttcttcctcg
    16261 gccttgcgcg cggcctcccg cgccgcctcc tcgatccgcc aggccgcgtc gatctcgtcg
    16321 gcctccgcct tctcctcctc cgagaggatg gcgcggtacg cgacgacgat ctcggcgttc
    16381 tcggcctgcg cgcggatcgt ggcgacgatc cgctcccgca ccggagcgtg cggctcgtcc
    16441 gcgatcaggg ggatgcagtc acggtccgcg aggagcatcc gggtgaagca gaccttcctt
    16501 tcctcgtccg gctccgaggc ctgctcgagg aggaagtaga agtcgcggtc gtggtcgaag
    16561 tcgttgccga cggtccggaa acggggcagc gcctcgtgga cgagctccga gaggagtccg
    16621 aggtacttct ctccgaggtc ccaggtctgc tccatcgaga cgacccggtc ccggaacatt
    16681 ttcgtaatcg ccattcgcgg tggtcctcgt ccgcgcggtg cgcgcggggt cgcgccgagt
    16741 ctagcaaccg gccggcccgg gcccgattcc gcccagcgcg aggatcgtcc gggcgtgccc
    16801 ctcgggcgag agctgcgcgc cggccgcctc cgcgccggcc gagagacggg gcccgaggcc
    16861 ctcgtggacg aagcggagca tcgccgcggc gaggagccgc gggtcgcccg gagggacgag
    16921 cagcccggag gtcccgtgac ggacgacgac cgagaggccg ggaacgtccg agccgatcac
    16981 cggccggccg tgcgccagcg cgtcgccggc gatcccggag ccggtcgcgt gccggtaggg
    17041 gcagacgacc gcgttgcacg cgtcgaa
//
LOCUS       DAOTEZ010000034        16863 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2317_length_16863_cov_6.420931, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000034 DAOTEZ010000000
VERSION     DAOTEZ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 16863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16863
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2317_length_16863_cov_6.420931"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="P5164_03015"
     CDS             <1..331
                     /locus_tag="P5164_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42896.1"
                     /translation="ATRRERRRAVVLLLGTDARDTGDLDAPRVRRYLERLRVPLHVWR
                     VALMAPPAAKDWPGAAEAVTTNGLGRAMDALRTDLASQRVVWVEGRLDALSVQATPRA
                     EGVVTLR"
     gene            354..833
                     /locus_tag="P5164_03020"
     CDS             354..833
                     /locus_tag="P5164_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HRY42897.1"
                     /translation="MTRIYVDADACPVKDEVYRVAKRYDLQVSVVSNGRMRVPDDPLV
                     EMVVVSGRFDAADAWIAERVTESDVAVTSDIPLASLCLAKGARVLDPKGRVFTEESIG
                     DALSNRELMAYLRDSGAITGGPAPFEARDRSRFLQKLDDLVQAARKAAGAAGVVSRP"
     gene            complement(820..3240)
                     /locus_tag="P5164_03025"
     CDS             complement(820..3240)
                     /locus_tag="P5164_03025"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRY42898.1"
                     /translation="MPGLGHEAGELLKRLEYRGYDSTGAAFVRADRSVKLLKRVGAPS
                     RVVPELGIDREAGQRFVGQVRWATYGAVTDANAQPHHVRCRVELVGAHNGNISSTDTL
                     RPRLSAGGHDVVSDNDGEALTHLAEDAFADGLADPAQLLAAGRAALAAAGLDVAVPER
                     ALLLIEAARRADAGVEGSYAAAYADPASEGIVAVKSGSSLYAGIGSDETGELVLVSSD
                     LTSVLSRTRMLVPLAEGEGIWFTEKAYVVFPLAGPIAFAAPRPRRSKLNVRDTGLRPP
                     FAYFMDQEIASSSENLEEIVRSYFRTPETEGLFAAFEDRVDLCKALVEKLLKLYGAAD
                     EASLGRGFRDLLGEPLLTELASRVRAHRGLLVSYGPFVSDEKALLADGVRVVPETSEA
                     AALLDLVLVWKKRRRVTALLQELVSAIRATQKEGGRVFLVASGTSYHAALTAGYFFNT
                     LAGVAVFPCNPGTFRSLYLNSLKPADLLIGISQSGETKDLVDVFQDVRARVPALRRVS
                     LVNNENSRIPQELSEFYLPILCGPEIAVAATKSFLNQVAVLYVVAASFSLNERRIVEK
                     LAAARALVSETLRRCEADVEEAAERLYLEPSLHILGTGLIGLAREGALKIREVVLNHA
                     EGYDAAEFKHGPNTILGKNTLFSIHDLAGVLEAWEERRGAGPFPGGLEALRLHPELVE
                     RHFSNYPLLFVCPPEERDVRITVSQIHTHKIRGADILVVSEPNEDLARAAAGRPAGDA
                     RYWSKGIEVPPSGDPALFVFAASVVLQRLAFRMSVKKMEYLDRLRVVDHGVHPDAPKN
                     VSKSITVD"
     gene            3520..3855
                     /gene="ytxJ"
                     /locus_tag="P5164_03030"
     CDS             3520..3855
                     /gene="ytxJ"
                     /locus_tag="P5164_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019636089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HRY42899.1"
                     /translation="MQPVRDAEELERAFGEPWALVFKHSTRCPVSSAAWREVAAFRKA
                     HPEAVVYVVHVPEQRSLSNALAARTGVRHESPQAILLEEGRVAWHDSHEGITAEALQG
                     LLPTVAVAG"
     gene            3947..5374
                     /locus_tag="P5164_03035"
     CDS             3947..5374
                     /locus_tag="P5164_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42900.1"
                     /translation="MISFVTLILGIAYGTWTVELSASPQVARIDLYVDGKLASEIAPP
                     WKTEVDLGHEITPREMVAVARGVDGARLGEARQWINRAKAYAEARFVLERDAAGRATR
                     ARLVWSCPTSPKPKAIDVSFDGIPIDALDPSRIPIPAYSSGSSHVLLADLTFDGGVVA
                     TAVASLGGGAKKDVERELTAFPVRLSGTRKRLPDPARLRGWFQVDGHDLAVAAVEKGT
                     AEVVFISSGLAFKELARLAGRESVEIFLKQDESGTQARSRTVVYRVRSAGKRRSQSKE
                     LDRDTVYSFLSPAPQATPGAAAETRLFPISEAFTSRDGPFLDVNGWAVFTPGDSLPRV
                     AEAVAVSGLSATRRERRRAVVLLLGRGDLEASDFDAGRAARYLARLGVPLHVWRLAPP
                     ESPVAPGWPKGLDVTTTEGLQAAFRTLREDLAAQRVIWLEGRVDPSKVEVSPVAQGMA
                     RALNGQDAPLPDRGGAPHLPRTSSE"
     gene            5577..6998
                     /locus_tag="P5164_03040"
     CDS             5577..6998
                     /locus_tag="P5164_03040"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRY42901.1"
                     /translation="MTRRPRLPRFLTNETARVAAVLIVCLAGHRAAARDLSLPEARER
                     VRSAHEALKAAGHELRVRQAEVGAADALRWPKLEASARVTRIDDPITFDLDPIRKVIL
                     SLHPAVPAALVPPFTLEVQDDLYERADVRLTWPLFAGGRIDAARDAARAREADAGAQL
                     RAVGDALATEVVRRYYAARLAERARAIRAEVLSALDGHLRNARRLEEEGFLSRAERLH
                     AEVARTEAERELKAAGHDLALAREALANVLSLPAEDADGLVTTTPLFVPAKLPPLPEL
                     KAAARDANPALARLEAAASLASAGLAAEKGRWYPEVALFGVRELYKPGLTLLDPTWAA
                     GLSARLTLFDGFSREKGIAAARERGDRVAELAARARRDVETLVEKRYREASKAREQVE
                     AFGAALELARENVRVRTRAFEEGVATSLDVVDARLSLAKVELGRLAAARDLDVALAEL
                     LEAAGQTETYETLRADGAGDVEK"
     gene            6995..7981
                     /locus_tag="P5164_03045"
     CDS             6995..7981
                     /locus_tag="P5164_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY42902.1"
                     /translation="MNERTKGLLALGGGVAVLGLLSLAALLLSRPGPETLQGEADATK
                     VDVAAKVAGRLATIDVKEGDAVEKGALVATIESPEIRAKLDQAEAARAGAVAQRDKAD
                     RGAREEEIRAAKSQWDRAKHAAELAETTFGRLERLARDGVVPAQRRDEAEAQWKTSRD
                     AAEAARAVYDMAVAGARSEDRSTAAAMVSRATGAVDEVRAFLDETKVVSPAAGEVYRR
                     NAEPGEVVPAGFPIVTLVDLTDVWVTFQVREDRLARLKMGATVPARFPALGGAEVPLT
                     VTFVAPQGDFATWRATSAQGGFDLKTFEVRARPAKPLPGLRPGMSAVVTWPR"
     gene            7969..9153
                     /locus_tag="P5164_03050"
     CDS             7969..9153
                     /locus_tag="P5164_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY42903.1"
                     /translation="MAPLASAPGVLRAARREAERLVASRHALVLLVFLPLAGFSLATA
                     IFWNRLPEELPVAVVDADRSLLSRRLVRMLDAAKSMRVAGPYATSGEALDEVLAGRAY
                     GIVSVPRHFERDVKRGAAPKVVLHYDALTMIPGSILKRDVRAVVGTLSAGVEIQARRA
                     LGETPDVAGARFEPVRSLRGALFNPGLDYRAFLLPAVLATLLSIFVLVAGVHALGVEL
                     RDGTAAEWLASGGSATRAVLGKLLPQALWFTAVGLAALAFPLRFLDVPLRGSASLVAL
                     ATLLLVLGYQAVALLLVAGSANLRFASSAAAFYGGPAFAFTGITFPTFGMPLAAQLWG
                     ALLPLTHFLALLLAQAMRGAPASASLSPLGALLAFVLVLPPLALWRMRRLMSDPAYWG
                     RT"
     gene            9150..10298
                     /locus_tag="P5164_03055"
     CDS             9150..10298
                     /locus_tag="P5164_03055"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY42904.1"
                     /translation="MRSLFRAFREEVSGSLRDPGALLVLVGAISLYAFFYPTPYRREV
                     LKEIPVAVVDLDRTPLSRQLVRLADAHELVRVAARAGSLAEAEALVRDGTAGGVLGIP
                     EGFEREVKRGAPATVGVLADATYFLVYRQVLTGLAESVGTLSAGIEIRRLTAQGMPEE
                     KARAFRDPVPLVSRPLFNPAEGYATYVVPPVLILILQQTLLIGIGLRGPADLGRLGSG
                     PLLARAAASVTGRTLAFVALYLVHALFYFGVVARLFGFPVRGSAAAAAGLVLPFLLAT
                     SFLGLALSVLFRRREAAIQTLLFTSLPAIFLAGFSWPPSALPGWLRVASQAIPSTSAI
                     PALLRVNQAGASLAHVRHELALLWGLAAVFFVLAVLAQAGRERAARRQ"
     gene            complement(10292..11059)
                     /locus_tag="P5164_03060"
     CDS             complement(10292..11059)
                     /locus_tag="P5164_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42905.1"
                     /translation="MDRFAARVGAGSFTAISRKRVADGLRVRIWAPSAIDQGTSSLCG
                     PASLLFDVATRDPEAYVRYVTSLWETGQGRIGRIDVRPGTDLKAFDPGRNVEASDWIA
                     LASLRDSENWFFDYQEDSDALAGITLPGELEGWFKKAGYTEVVNDARAIVDKDEACLR
                     RADDYFRNGFRVCLFIHSNMLSPSTQGNGSATADHWVVQTGRVTFGATMIEGKPATTV
                     SLRIFTWGEGRRAVPQAGVLPLDAFLDNFYGFVAARY"
     gene            complement(11245..12072)
                     /locus_tag="P5164_03065"
     CDS             complement(11245..12072)
                     /locus_tag="P5164_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42906.1"
                     /translation="MQDNVEVTVRRADVGLARRRGLAGALALLVAFPAAANLRAPRRV
                     PESPSSALAAPATRLVVERERLTFLCGAEACDVLAEYDVTAVGAEHVRLEMILPAPGD
                     VTARTNGSPETVSIVPAEPLREEELRGLPEPGPGEPPLHRAAFDGVLRPGPNTIAVRY
                     RQPLGGEEVGHSGRRAGRFVQRFRYELWPLREWTRKEGFRLRLAIGVERPAPSLWQRW
                     FGHPRSVSCLTSDPAAPAVEGRLEQRGAHLWLEAELGPSFPDRITCYVGDDDLMPRY"
     gene            complement(12139..13323)
                     /locus_tag="P5164_03070"
     CDS             complement(12139..13323)
                     /locus_tag="P5164_03070"
                     /inference="COORDINATES: protein motif:HMM:NF019585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HRY42907.1"
                     /translation="MSAPHAYERDPYLKELPVRVLATGSDDGRSWAVLDDTVLFPEGG
                     GQPADHGLLGDVPVVDVRTVEGRAVHFLEAPVPEGPTLLRLEWARRFDHMQQHTGQHL
                     LTAVAADRFGWETTAFHLGPETCDVELAVPSISPADLERLEEAVAACIRAAVPVTARR
                     VLPEELPGLAVRSRGLPEGHSGDVRLVEIAGVDLNTCGGTHLRSTAELEGLKLLGTER
                     LRGGTRLLFVSGGRVRRRLGAAEERNARLRVLLGAPDPGLAEAVAVKLAQLAEAQKAL
                     RGAEEELAEAKAEALAAAGLPVVDAHFEGRDLVFLGRVAKQFAELPVGGLAFLTGERD
                     GVGVFVLAAGAGSSSDVRALGSAVAAELGGRGGGSGKLFQGKASLERRAGALTLLRTP
                     SP"
     gene            complement(13391..13981)
                     /locus_tag="P5164_03075"
     CDS             complement(13391..13981)
                     /locus_tag="P5164_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HRY42908.1"
                     /translation="MELLDSGRSVLLVIDLQGKLMEVIHRPRLVVEATLRLMKLAELF
                     GVPVVLTEQYPKGLGPTHPEIRSGFDALTVPKRYVDKTAFGCCGEPAFLRALDELRPR
                     ARAPRQVVVTGIEAHVCVLQTVVELIHHGNQVHVAWDAVSGRGEEYRHHALERMAAAG
                     AAITNHESAGFEWARDKNHPRFRDLSALLKDGQPSG"
     gene            complement(14053..15177)
                     /locus_tag="P5164_03080"
     CDS             complement(14053..15177)
                     /locus_tag="P5164_03080"
                     /inference="COORDINATES: protein motif:HMM:NF037527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42909.1"
                     /translation="MPKKPTPDDLRNLLAPRTEPCISLFLPLPRHGAELAMAPVRFRN
                     LVRQAEALLAERYDARRIRELIAPLGGLAEAVDVFTPGPEGLAAFRADGVLETYRLWP
                     PPPERAVVADSFHVKPLLRSLQDRNRYHVLALGSRRVALFDGSERGLVPRVLPGLPAP
                     LEPSAASGGRGGGGRASRGGPGKVPAEGEGGRREETLRLFRAADGALREALPGATPIL
                     LAGSGSVLPAFRDVCTHPAVFPEGVEGAVDDVGIDELHERVLPTALRALEARNARLVE
                     DFRKAQDRGLASDVLTTVAEAAVAGRVRRLVVAGGKTLFGRVDRETGEVTLHAGQSGP
                     QDDDVLDDLAETVLARGGEVFVLDATKMPHEAAAVAIYRW"
     gene            15276..16778
                     /locus_tag="P5164_03085"
     CDS             15276..16778
                     /locus_tag="P5164_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY42910.1"
                     /translation="MRRPDRALAALLLAGLLVAGGCSRSGSGRELHVVWAMDVPSLDP
                     NERFDVVTATVASNVFEPLVRYDRQMAFEPCLAKAWEIRDGAVWRFRLREGVSFHDGT
                     ELSAEDVAFSIDRLRAQPAWDIHRYVSAIKDVTVVDPLTVDIRSERPAGLLSVLSYVY
                     ILPKRSVESAGEAEYFKAPAGTGPYRIASWKPGKSLRLEAFPGYWEGPPEIPAAVFRV
                     EKDDDGIWTEAKRLAPAILFGPTTTTWIRHREDPAFRLVEQPSLAVHYLALRVGGGPE
                     NPLSDVRVRRALRASIDYEALLAALPARGAFPASQFVPPAIVGFDPSLAVPAFVPGAA
                     RRMIEETGLRQTKPLVFLTAGGGGSISRYLLKAFSDAGLSVREESVTPEEYETRLAAC
                     DADIFLTGWICTTGDAGELFEGNFYRRGSTQNPCGYGCDQMDTAIEEIGRTLDPAVRR
                     DLLQSAMRTLVDDLPWVPLLVSYERHALTGGVEWRTRADGQLDLRDVRIR"
BASE COUNT         2206 a         6292 c         6018 g         2347 t
ORIGIN      
        1 ggcaacccgc cgcgagcgcc ggcgagccgt cgtcctcctg ctcggcaccg acgcccgcga
       61 tacgggcgac ctcgacgccc cacgggtccg caggtacctc gagaggctcc gggtccccct
      121 tcacgtgtgg cgggtcgcgc tgatggcacc gcctgccgca aaggactggc ccggcgccgc
      181 cgaggccgtc acgacgaacg gcctgggaag agcgatggac gccctccgga ccgacctcgc
      241 gtcccagcgc gtcgtctggg tcgaggggcg cctggacgct ctctccgtcc aagcgacccc
      301 gcgggcggaa ggcgtagtga cgctccggtg aagcggtcgg gaaggcacct cgggtgaccc
      361 ggatctacgt cgacgccgac gcgtgccccg tgaaggacga ggtctaccgc gtcgcgaagc
      421 gctacgacct ccaggtctcc gtcgtctcga acggacgcat gcgcgtcccc gacgacccgc
      481 tcgtcgagat ggtcgtcgtg tccggccgct tcgacgcggc cgacgcctgg atcgccgagc
      541 gcgtgacgga gagcgacgtc gccgtgacgt cggacatccc gctcgcttcg ctttgcctcg
      601 cgaagggcgc gcgggtgctg gacccgaaag gacgggtctt cacagaagaa tcgatcggcg
      661 acgctctgtc gaaccgcgag ctgatggcct atctccgcga ttcgggagcc attaccggcg
      721 gcccggcccc gttcgaggcc cgcgaccgct cgcggttcct gcagaagctg gacgacctcg
      781 tccaggccgc ccggaaggcg gccggcgccg cgggtgtcgt cagtcgaccg taatcgactt
      841 cgagacgttc ttcggggcgt cggggtggac gccgtggtcg acaacccgga ggcggtcgag
      901 gtattccatc ttcttcaccg acatccggaa ggcgagccgc tggaggacca cgctcgcggc
      961 gaagacgaag agggcggggt cgccggacgg cgggacctcg atccccttcg accagtagcg
     1021 cgcatccccc gcgggccgcc ccgccgcggc ccgggcgagg tcctcgttcg gctcggaaac
     1081 gacgaggatg tcggcgcccc ggatcttgtg ggtgtggatc tggctcaccg tgatcctcac
     1141 gtcgcgctcc tcgggcgggc agacgaagag gagcgggtag ttcgagaagt gccgctcgac
     1201 gagctcgggg tggagccgca gggcctcgag gccgccgggg aacggccccg cgccacggcg
     1261 ctcctcccac gcctcgagga cgccggcgag gtcgtggatc gagaagagcg tgttcttccc
     1321 caggatcgtg ttcggcccgt gcttgaactc cgccgcgtcg tacccctcgg cgtggttcag
     1381 gacgacctcc cggatcttga gcgccccctc gcgcgcgagg ccgatcaggc cggtgccgag
     1441 gatgtggagg ctcggctcga ggtagaggcg ctcggccgcc tcctccacgt ccgcctcgca
     1501 gcggcggagc gtctccgaga cgagggcgcg ggccgccgcg agcttctcga cgatccgacg
     1561 ctcgttcagc gagaaggacg ccgcgacgac gtagaggacg gcgacctggt tcagaaagct
     1621 cttcgtcgcg gccaccgcga tctccggccc gcagaggatc gggaggtaga actccgagag
     1681 ctcctgcggg atccggctgt tctcgttgtt gacgagcgag acgcgacgca gggcggggac
     1741 gcgggcccgg acgtcctgga agacgtcgac gaggtccttc gtctcccccg actgcgagat
     1801 gccgatcagg aggtccgccg gcttcaggga gttcaggtag agggagcgga acgtcccggg
     1861 attgcacggg aagacggcga cgccggcgag cgtgttgaag aaatagccgg ccgtcagcgc
     1921 ggcgtggtag gaggtccccg aggcgacgag gaagacccgc cccccttcct tctgggtcgc
     1981 gcggatcgcc gagacgagct cctggaggag ggccgtgacg cgcctccgct tcttccagac
     2041 gaggacgaga tcgaggagcg ccgctgcttc ggaggtctcg gggacgacgc gcacgccgtc
     2101 ggcgaggagc gccttctcgt cggagacgaa cggaccgtag gagacgagaa gaccccggtg
     2161 tgcgcggacc cgggaggcga gctccgtcag gagcggctcc ccgaggaggt cccggaaacc
     2221 gcgcccgagg ctcgcctcgt ccgcggcccc gtagagcttc aggagcttct cgacgagcgc
     2281 cttgcagagg tccacgcggt cttcgaacgc ggcgaagagc ccctccgtct ccggcgtccg
     2341 gaagtaggaa cggacgatct cctcgaggtt ctcagacgag gaggcgatct cctggtccat
     2401 gaagtaggcg aacggcggcc ggaggccggt gtcgcgcacg ttcagcttcg agcggcgggg
     2461 cctcggcgcg gcgaacgcga tcgggcccgc gagcgggaag acgacgtacg ccttctcggt
     2521 gaaccagatc ccctccccct cggcgagcgg gacgagcatc cgggtccgcg agaggaccga
     2581 cgtcaggtcg ctcgagacga ggacgagctc tcccgtctcg tccgacccga tcccggcgta
     2641 gagggacgac ccggacttca ccgcgacgat cccttccgag gccgggtcgg cgtaggcggc
     2701 ggcgtacgag ccctcgaccc ccgcgtcggc gcggcgcgcg gcctcgatca ggaggagcgc
     2761 ccgttcgggg accgcgacgt cgaggccggc cgccgcgagc gccgcccgcc ccgcggcgag
     2821 gagctgcgcc gggtcggcga ggccgtcggc gaaggcgtcc tccgcgaggt gggtgagcgc
     2881 ctcgccgtcg ttgtcggaga cgacgtcgtg cccgcccgcg gaaagccgcg gccggagcgt
     2941 gtcggtgctc gagatgttgc cgttgtgggc gccgacgagc tcgacgcggc acctcacgtg
     3001 gtgcggctgg gcgttcgcgt ccgtgacggc cccgtaggtc gcccaccgga cctggccgac
     3061 gaaccgctgc cccgcctccc ggtcgatccc gagctccggg acgacgcggc tcggcgcgcc
     3121 gaccctcttg aggagcttca cgctccggtc ggcgcggacg aaggccgccc ccgtcgagtc
     3181 gtacccccgg tactccaggc gcttcagcag ctcccccgcc tcgtggccga ggccgggcac
     3241 ctctcgtccg tgacagaccg aaacgatgcc gcacatctgc gctcactccc ctcgttccga
     3301 cggtgccgca cgagccgtgc gcgggcggga cgtcccggtc cgcggcgttg aggcgtcccc
     3361 ggcgacacgt cggacctctc atcttgcctc acgccgcccg cccgcgcgag atcgaaggcc
     3421 gcgccggcgc cgcgcgccgg gacgggagat ccgctcgctt gaccgcccgc ccccgctcgc
     3481 ctacaacggt cgcgcggggc ctctcccgcg gaggagatca tgcagcctgt ccgggatgcc
     3541 gaggagctcg agcgagcctt cggggagccg tgggccctcg tcttcaagca ctccacccgc
     3601 tgccccgtga gctcggcggc ctggcgggag gtggcggcgt tccggaaggc gcatccggag
     3661 gccgtcgtct acgtcgtcca cgtcccggag cagcggtcgc tttcgaacgc cctcgccgcg
     3721 cggaccggcg tccgccacga gtcgccgcag gcgatcctcc tcgaggaggg ccgcgtcgcg
     3781 tggcacgact cgcacgaggg gatcaccgcc gaggcgctcc aggggcttct ccctacggtc
     3841 gccgtcgccg ggtgagggcg gcgccccgct tcgggcgggg caccgtcctt ccggaaggac
     3901 gtcgatctcc gcacacatct tgcttatcgg cgtgcgggag aggcgcatga tttccttcgt
     3961 cacgctgatc ctcggaatcg catacgggac ctggacggtc gagctttcgg cgagcccaca
     4021 ggtcgcgcgg atcgatctct acgtcgacgg gaagctcgcc agtgagatcg ctcctccctg
     4081 gaagacggag gtcgacctcg gacacgagat cacgccccgc gagatggtcg cggtcgctcg
     4141 gggggtggat ggtgcccggc tcggcgaggc ccgccagtgg atcaaccggg cgaaggccta
     4201 cgccgaagcg cggttcgtcc tcgagcgcga cgcggccggc agggcgaccc gcgcacgtct
     4261 cgtctggagc tgtccgacga gtccgaagcc gaaggcgatc gacgtctcgt tcgacgggat
     4321 accgatcgac gcgctcgatc cctcgcggat cccgatcccc gcctactcga gcggctcttc
     4381 ccacgtcctc ctcgccgacc tgacgttcga cgggggcgtc gtcgccacgg ccgtcgcgag
     4441 cctcggcggc ggggcgaaga aggacgtcga gcgcgagctg accgccttcc cggtccggct
     4501 ctccggtacg aggaagagac tccccgaccc ggcccggctc cggggctggt tccaggtcga
     4561 cgggcacgac ctcgccgtcg cggccgtcga gaaggggacc gccgaagtcg tcttcatctc
     4621 gtcggggctc gccttcaaag agctcgcacg actggccgga agggagtctg tcgagatctt
     4681 cctgaaacag gacgagtccg gcacccaagc gcgctcgcga actgtcgtgt accgcgtgag
     4741 gtccgctggg aagcgccgtt cgcagtcgaa ggaactcgac cgcgacacgg tctacagctt
     4801 cctctccccg gccccgcagg cgactccggg ggccgccgcg gagacgaggc tctttcccat
     4861 ctccgaggcc ttcacatcgc gcgacggtcc gttcctcgac gtcaacggtt gggcagtctt
     4921 cacgccgggc gactcgctcc ctcgcgtcgc cgaggccgtc gccgtctccg ggctcagcgc
     4981 gactcgccgc gagcggcgtc gggccgtcgt cctcctcctc ggacgcgggg atctggaggc
     5041 gagcgacttc gacgccggac gggcggcacg gtacctcgcg cgcctggggg tccctctcca
     5101 cgtctggcgg ctcgcccctc cggagtcgcc cgtcgcgccg ggctggccca agggtctcga
     5161 cgtcacgacg accgaggggc tccaggccgc cttccggacc ctccgcgagg acctcgccgc
     5221 ccagcgcgtg atctggctcg agggacgcgt cgacccgtcg aaggtcgaag tgtcccccgt
     5281 cgcccagggc atggcccgcg cgctgaatgg ccaggatgcc cctctcccgg atcgaggcgg
     5341 agctccgcac cttccgcgaa cctcttccga gtgagctccg ggcgatgacg atcgtgggcg
     5401 ggtgagggcc ttccgcaggg attcccgacg gacgacgcac gcgacgcaac gacctcgcag
     5461 caccccaccc ggattgcctg gcccgacgca tcatcctcct tcgcgcgtat gatccttcgc
     5521 cggcggggcg acggcgcctc gcggcgcccg atcgcctcgc gcaaggagtt ccgctcttga
     5581 cgaggcgtcc ccggctcccc cgcttcctca caaacgagac ggcgcgcgtc gcggccgtcc
     5641 tgatcgtctg cctggccgga caccgcgccg cggcgcgcga cctctcgctc cccgaggcgc
     5701 gggagcgcgt ccgctccgcc cacgaggcgc tcaaggcggc cggacacgag ctgcgggtcc
     5761 ggcaggccga ggtcggcgcc gccgacgccc tccgctggcc gaagctcgag gcgagcgcga
     5821 gggtcacgcg gatcgacgac ccgatcacgt tcgacctcga cccgatccgg aaggtgatcc
     5881 tctctctcca tccggccgtg ccggccgccc tcgtcccgcc gttcacgctc gaggtccagg
     5941 acgacctcta cgagcgggcg gacgtccggc tgacgtggcc cctcttcgcg ggagggagga
     6001 tcgacgccgc gcgtgacgcc gcgcgggccc gcgaggcgga cgccggggcg cagcttcgcg
     6061 ccgtcggcga cgccctcgcc accgaggtcg tccgccgcta ctacgccgcg aggctcgccg
     6121 aacgggctcg tgccatccgc gccgaagtcc tctcggcgct cgacgggcac ctccggaacg
     6181 cgcgccgcct cgaggaggag gggttcctct cgcgcgcgga gcggcttcac gccgaggtcg
     6241 cacggaccga ggcggagcgg gagctgaagg cggccgggca cgacctcgcg ctcgcccgcg
     6301 aggcgctcgc gaacgtcctc tcgctcccgg ccgaggacgc ggacggactc gtcacgacga
     6361 cgccgctctt cgtccccgcg aagctgccgc cgcttccgga gctgaaggcg gccgcccggg
     6421 acgcgaaccc ggccctcgcc cggctcgagg ccgcggcctc gctcgcgagc gccggcctcg
     6481 ccgccgagaa gggccgctgg tatcccgagg tcgcgctctt cggcgtgcgc gagctctaca
     6541 agccggggct gacgctcctc gacccgacct gggccgccgg gctcagcgcc cgcctcaccc
     6601 tcttcgacgg cttctcccgc gagaagggga tcgcggcggc gcgggagcgc ggcgaccgcg
     6661 tcgccgagct ggcggcgcgc gcccggcggg acgtcgagac gctcgtcgag aagcggtatc
     6721 gcgaggcgag caaggcccgg gagcaggtcg aggcgttcgg cgcggcgctc gagctcgcgc
     6781 gggagaacgt ccgggtgcgg acccgggcgt tcgaggaggg ggtcgcgacg tcgctcgacg
     6841 tcgtcgacgc gcgcctctcc ctggcgaagg tcgagctcgg ccgcctcgcg gcggcccgtg
     6901 acctcgacgt cgccctcgcc gagctcctcg aagcggccgg acagaccgaa acgtacgaga
     6961 cgctccgcgc ggacggggcg ggggacgtgg agaagtgaac gaacgaacga agggactcct
     7021 cgcgctcggg ggcggcgtcg ccgtcctcgg gctcctctct ctcgccgccc tcctcctctc
     7081 gcgccccggc ccggagacgc tccaggggga ggccgacgcc acgaaggtcg acgtcgccgc
     7141 gaaggtcgcc ggacggctcg ccacgatcga cgtgaaggag ggagacgccg tggagaaggg
     7201 cgccctcgtc gcgacgatcg agagccccga gatccgggcg aagctggacc aggcggaggc
     7261 cgcgagggcg ggcgccgtcg ctcagcgcga caaggccgac cggggcgccc gcgaggagga
     7321 gatccgcgcg gccaagagcc agtgggaccg ggcgaagcac gccgcggagc tcgccgagac
     7381 gacgttcgga cgcctcgagc gcctcgcgcg cgacggcgtc gtcccggctc agcggcgtga
     7441 cgaggccgag gcgcagtgga agacgtcccg cgacgccgcc gaggcggccc gggccgtcta
     7501 cgacatggcc gtcgccggcg cgcgaagcga ggatcgctcg accgccgccg cgatggtctc
     7561 gcgcgcgacg ggcgccgtcg acgaggtgag ggccttcctc gacgagacga aggtcgtctc
     7621 tccggccgcg ggcgaggtct accggcggaa cgccgagccg ggcgaggtcg tcccggccgg
     7681 cttcccgatc gtgacgctcg tcgacctgac ggacgtctgg gtgacgttcc aggtgcggga
     7741 ggaccggctc gcgcggctga agatgggggc caccgtcccg gcccgcttcc ccgccctcgg
     7801 cggcgcggag gtcccgctga cggtcacgtt cgtcgcgccg cagggggact tcgcgacgtg
     7861 gcgcgcgacg agcgcgcagg ggggcttcga cctgaagacg ttcgaggtcc gcgcccgccc
     7921 ggcgaagccg ctgcccggcc tccgccccgg gatgagcgcc gtcgtcacgt ggccccgcta
     7981 gcgagcgcgc cgggcgttct ccgcgcggcg cggcgggagg cggagcggct cgtggcgagc
     8041 cgccacgccc tcgtcctcct cgtcttcctg ccgctcgcgg gcttctctct cgctacggcg
     8101 atcttctgga accggctccc ggaggagctc ccggtcgccg tcgtcgacgc cgaccgctcc
     8161 ctcctctcgc gccgcctcgt ccggatgctc gacgcggcga agtcgatgcg cgtcgcgggg
     8221 ccgtacgcga cgagcggcga ggctctcgac gaggtcctcg cgggacgcgc ctacggcatc
     8281 gtgtcggttc cacggcactt cgagcgggac gtgaagaggg gcgccgcgcc gaaggtcgtc
     8341 ctccactacg acgcgctcac gatgatcccc gggagcatcc tgaagcggga cgtgcgggcg
     8401 gtcgtcggga cgctctcggc cggcgtcgag atccaggccc gcagggcgct cggcgagacg
     8461 cccgacgtcg cgggggcgcg cttcgagccg gtgaggtcgc tccgcggcgc gctcttcaac
     8521 cccgggctcg actaccgcgc gttcctcctc cccgcggtcc tcgcgacgct cctttcgatc
     8581 ttcgtcctcg tcgccggcgt ccacgccctc ggcgtggagc tgcgcgacgg gacggccgcg
     8641 gagtggctcg cctcgggcgg gagcgcgacg cgcgccgtcc tcggcaagct cctcccgcag
     8701 gcgctctggt tcacggcggt cggcctcgcc gctctcgcgt ttccgctccg cttcctcgac
     8761 gtcccgctcc gcggcagcgc gtccctcgtc gcgctcgcga cgctcctcct cgtcctcggc
     8821 taccaggccg tcgcgctcct cctcgtcgcc gggagcgcga acctccgatt cgcctcgagc
     8881 gcggcggcct tctacggagg gcccgccttc gcgttcaccg ggatcacctt cccgacgttc
     8941 gggatgccgc tcgccgcgca gctctggggg gcgctcctcc ctctcaccca cttcctcgcc
     9001 ctcctcctcg cgcaggcgat gcgcggcgcc cccgcctcgg cgtccctctc gccgctcggg
     9061 gcgctcctcg cgttcgtcct cgtcctcccg cctctcgccc tgtggcgaat gcggcgcctc
     9121 atgagcgacc cggcgtactg gggacggacg tgaggagcct ctttcgggcc ttccgcgagg
     9181 aggtctcggg ctccctccgc gacccgggcg ccctcctcgt cctcgtcggg gcgatctcgc
     9241 tctacgcgtt cttctacccg acgccgtacc gacgcgaggt gctgaaggag atcccggtcg
     9301 ccgtggtcga cctcgaccgg acgcccctct cccgccagct cgtccgcctc gccgacgccc
     9361 acgagctcgt ccgcgtcgcc gcgcgcgccg ggagcctcgc ggaggccgag gcgctcgtcc
     9421 gcgacgggac ggcgggcggc gtcctcggca tcccggaggg cttcgagcgc gaggtgaagc
     9481 gcggcgctcc ggcgacggtc ggcgtcctcg ccgacgcgac ctacttcctc gtctaccggc
     9541 aggtcctcac cggcctcgcg gagtcggtcg ggacgctctc ggccggcatc gagatccggc
     9601 ggctgacagc gcaggggatg cccgaggaga aggcgcgcgc gttccgcgac ccggtcccgc
     9661 tcgtctcgcg ccccctcttc aacccggccg agggatacgc gacgtacgtc gtcccgccgg
     9721 tcctcatcct gatcctccag cagacgctcc tgatcgggat cggcctgcgc ggccccgccg
     9781 acctcggccg gctcggctcc ggcccgctgc tcgcccgggc cgcggcctcg gtgacgggga
     9841 ggacgctcgc gttcgtcgcc ctctacctcg tccacgcctt gttctacttc ggcgtcgtcg
     9901 cgcgcctgtt cggcttcccc gtccgcggga gcgcggcggc cgccgccggc ctcgtcctcc
     9961 cgttcctcct cgcgacgagc ttcctcgggc tcgcgctctc cgtcctcttc cggcggcgcg
    10021 aagcggcgat ccagacgctc ctcttcacgt ccctcccggc gatcttcctc gccggattct
    10081 cgtggccgcc gtcggcgctg cccggctggc tgcgggtcgc ctcgcaggcg atcccctcga
    10141 cgagcgcgat cccggcgctc ctccgcgtga accaggcggg cgcctccctc gcgcacgtgc
    10201 ggcacgagct cgcgctcctc tgggggctcg cggccgtctt cttcgtcctc gccgtcctcg
    10261 cgcaggccgg ccgggagagg gcggcccgcc gtcagtagcg ggcggcgacg aagccgtaga
    10321 agttgtcgag gaaggcgtcg agcgggagga cacccgcctg cggcaccgcg cggcgcccct
    10381 cgccccacgt gaagatccgg agcgagacgg tcgtggccgg cttcccctcg atcatcgtgg
    10441 ccccgaacgt gacgcgcccg gtctggacga cccagtggtc ggcggtcgcc gacccgttcc
    10501 cctgcgtcga cgggctcagc atgttggagt ggatgaagag gcagacgcgg aatccgttcc
    10561 ggaagtagtc gtcggccctt cggaggcacg cctcgtcctt gtcgacgatc gcccgcgcgt
    10621 cgttgacgac ctccgtgtag ccggccttct tgaaccaccc ctcgagctcg cccggcagcg
    10681 tgattccggc gagcgcgtcg gagtcctcct ggtagtcgaa gaaccagttc tccgagtccc
    10741 tgaggctcgc gagggcgatc cagtcggagg cctcgacgtt tcgccccggg tcgaaggcct
    10801 tcaggtcggt gccgggccgc acgtcgatgc ggccgatccg gccctggccc gtctcccaga
    10861 gcgacgtgac gtaacggacg tacgcctccg gatcgcgcgt cgcgacgtcg aagaggaggg
    10921 acgccggccc gcagaggctc gaggtcccct ggtcgatggc gctcggcgcc cagatgcgca
    10981 cgcggagacc gtcggcgacc cgcttccgcg agatcgcggt gaaggagccc gcaccgacgc
    11041 gcgcggcgaa ccggtccacc agcttctcgg cacgctgctg gggcgttgcc gaccggcctt
    11101 ccggtccggc gtcgtcctcg ggcgcgaccc gggcgccgcg tctgatcggg ccgggatagg
    11161 cgaaggagcg acccaggagc ccttccggga ccgggtcgtg aggggagtcc gttccgcggg
    11221 gaccagggag attcgatcca gccatcagta cctcggcatc aggtcgtcgt caccgacgta
    11281 gcaggtgatc cgatcgggaa aggaagggcc gagctccgct tcgagccaga ggtgcgcgcc
    11341 ccgctgctcg agccgcccct cgacggccgg agccgccggg tcgctcgtga ggcaggaaac
    11401 cgaccgcggg tggccgaacc agcgctgcca gaggctcggg gcggggcgct cgacgccgat
    11461 cgcgagccgg agccggaagc cttccttccg cgtccactcg cgcaggggcc agagctcgta
    11521 ccggaagcgc tgcacgaacc tcccggcgcg gcggcccgaa tgtccgacct cctcgccccc
    11581 gagcggctgc cggtaccgga ccgcgatcgt gttcggaccc ggccggagca cgccgtcgaa
    11641 agcggcccgg tgaagcggcg gctcccccgg ccccggctcc ggcaggccgc gcagctcttc
    11701 ttcccggagc ggctccgccg ggacgatgct caccgtctcc ggcgatccgt tcgtccgggc
    11761 cgtcacgtcg cccggggcgg gcaggatcat ctcgagccgg acgtgctccg cgccgacggc
    11821 cgtgacgtcg tattcggcca ggacgtcgca cgcctccgcc ccgcagagga acgtcagccg
    11881 ctcgcgctcc acgacgagcc gtgtcgcggg cgctgcgagg gcggacgagg gggactccgg
    11941 gacccgccgg ggagcgcgca ggttcgcggc ggccgggaac gcgacgagca gcgcgagcgc
    12001 gccggcgagg cctcgccggc gtgcgaggcc gacgtcggcc cgacgcaccg tcacctcgac
    12061 gttgtcctgc acgcgagcag cctacgcccg ctcccggacc ggctcaagaa ggccgcgggc
    12121 cccgtggcgt gatccgggtc acggcgaagg cgtgcgtagc agagtcaggg ccccggcgcg
    12181 gcgctcgagg ctcgccttcc cctggaagag cttcccggac ccgccgccgc gcccgcccag
    12241 ctccgcggcc acggcggaac cgagagcccg gacgtcggag ctcgatcccg cgcccgccgc
    12301 gaggacgaag actccgaccc cgtccctctc ccccgtcagg aaggcgagcc ctccgacggg
    12361 cagctccgcg aactgcttcg cgactcgccc gaggaagacc aggtcccgcc cctcgaagtg
    12421 cgcgtcgacg acgggcagcc ccgccgcggc gagcgcctcc gccttcgcct cggcgagctc
    12481 ctcttcggcg ccgcgcagcg ccttctgcgc ctcggccagc tgcgccagct tcaccgcgac
    12541 cgcctcggcg aggcccggat cgggcgctcc gagaaggacg cggaggcgcg cgttgcgctc
    12601 ctcggccgcg ccgagcctcc tccggacgcg tcccccggag acgaagagga ggcgagtccc
    12661 cccgcggagc cgctcggtgc cgagcagctt caagccctcg agctccgccg tcgaccggag
    12721 gtgcgtcccg ccgcaggtat tgaggtcgac gcccgcgatc tcgacgagcc gcacatcgcc
    12781 cgaatgccct tccggcaggc cgcgggaacg cacggcgaga cccggcagct cctcggggag
    12841 gacacgccgc gccgtcaccg ggacggctgc ccggatgcaa gccgccaccg cctcctcgag
    12901 acgctcgagg tcggccggag agatcgacgg gacggcgagc tcgacgtcgc acgtctccgg
    12961 accgaggtgg aacgccgtcg tctcccatcc gaagcggtcg gcggccacgg cggtgaggag
    13021 gtgctgcccc gtgtgctgct gcatgtggtc gaaccgcctc gcccattcga ggcggaggag
    13081 cgtcggcccc tcggggaccg gagcctcgag gaaatggacg gcgcggccct ccaccgtccg
    13141 gacgtcgacg accggaacgt cgccgaggag cccgtggtcc gcgggctgcc cgcccccctc
    13201 gggaaagagg acggtatcgt cgagaacggc ccacgaacgc ccgtcgtcgc ttcccgtcgc
    13261 gaggacgcgg acggggagct ctttgaggta cgggtcgcgt tcgtacgcgt gcggagcgct
    13321 catgggcgaa gagtaccctc gagcgccttc cgccgcgcct ctccccgcgg cgggagaggg
    13381 cgcgcgtccg tcagccggag ggctggccgt ccttcaggag ggccgagagg tcgcgaaacc
    13441 tcgggtggtt cttgtctcgc gcccattcga agccggcgga ttcgtggttc gtgatcgccg
    13501 ccccggccgc ggccatccgc tcgagcgcgt gatgccggta ctcctcgccc cggccgctga
    13561 ccgcgtccca cgcgacgtgg acctggttcc cgtggtggat cagctcgaca acggtctgca
    13621 ggacgcagac gtgagcctcg atcccggtga cgacgacctg tcgcggcgcg cgcgccctcg
    13681 gccggagctc gtcgagcgcc cggaggaacg ccggctcgcc gcagcagccg aaggcggtct
    13741 tgtcgacgta cctcttcggg acggtgaggg cgtcgaaccc ggagcggatc tccgggtggg
    13801 tcggcccgag ccccttcggg tactgctcgg tcaggacgac cggcacgccg aacagctcgg
    13861 cgagcttcat caggcgcagc gtcgcctcga cgacgaggcg ggggcgatgg atcacctcca
    13921 tcagcttccc ctgaaggtcg atcaccagaa ggacgctgcg tcccgagtcc aggagctcca
    13981 tccctcacct ccccgccggc ccggggccgg ccgtcggcgg ggccgcggcc tcctgccgcg
    14041 gccccgcgtt gctcaccacc tgtagatcgc gacggccgca gcctcgtgcg gcatcttcgt
    14101 cgcgtcgagg acgaagacct ctccgccgcg ggcgaggacc gtctcggcga ggtcgtcgag
    14161 gacgtcgtcg tcctggggtc ccgattgtcc cgcgtggagc gtcacctcgc ccgtctcccg
    14221 gtcgacccgg ccgaagagcg tcttcccgcc ggcgacgacg aggcgccgga cgcgccccgc
    14281 gacggccgcc tccgcaacgg tcgtcagcac gtcgctggcg aggccccggt cctgggcctt
    14341 tcgaaagtcc tcgacgagcc gggcgttcct ggcttcgagg gcgcggagcg cggtcggaag
    14401 gacccgttcg tggagctcgt cgatgccgac gtcgtccacc gcgccctcga ccccttcggg
    14461 aaagaccgcc ggatgcgtgc agacatcccg gaacgcggga aggacggagc cggagcccgc
    14521 gagcaggatc ggcgtcgctc ccgggagcgc ctcacggagg gcgccgtccg cggcgcggaa
    14581 gagccgaagc gtctcttcgc ggcggccgcc ctccccttcg gccgggacct ttccggggcc
    14641 gcctctcgag gctctcccgc ctccgccccg gcccccgctc gccgcggatg gctcgagcgg
    14701 cgccggaagg cccgggagga cgcgcgggac gagccccctc tccgagccgt cgaaaagcgc
    14761 gacgcggcgg gagccgaggg cgaggacgtg gtagcggttc cggtcctgca ggctccgaag
    14821 gagcggcttg acgtggaagc tgtcggcgac caccgcccgc tcgggcgggg gcggccagag
    14881 cctgtaggtc tcgaggaccc cgtccgcgcg gaacgccgcg agcccctccg ggcccggggt
    14941 gaagacgtcg acggcttccg ccagcccgcc gagcggcgcg atcagctcgc ggatacgtct
    15001 cgcgtcgtac cgctccgcga ggagcgcctc ggcctgacgt acgaggttcc ggaagcggac
    15061 cggcgccatc gccagctcgg cgccgtgccg gggcagcgga aggaagagag agatgcacgg
    15121 ctccgtcctc ggagcgagaa ggttccggag gtcgtccggt gtcggcttct tcggcatgag
    15181 cggtcctccc tcgttcggag gacaacgata tgttctccga cgacattccc gccacccgtc
    15241 tgacgcccgg ggtccggggc gggtaacatg cggccgtgcg gaggcccgat cgcgcgctgg
    15301 ccgcgttgct cctggctggg ctcctcgtcg ccggaggatg ctcccggagc gggagcggcc
    15361 gagagctgca cgtcgtgtgg gcgatggacg tcccgtcgct cgacccgaac gagcggttcg
    15421 acgtcgtgac ggcgacagtc gcctcgaacg ttttcgagcc tctcgtccgc tacgaccggc
    15481 agatggcgtt cgagccgtgc ctcgcaaagg cgtgggagat ccgcgacggc gccgtgtggc
    15541 ggttccggct ccgcgagggg gtctcgtttc acgacgggac ggagctctcc gcggaggacg
    15601 tcgccttctc gatcgatcgc ctccgggccc agcccgcctg ggacatccac cgttacgtgt
    15661 cggcgatcaa agacgtgacg gtcgtggacc cgctcaccgt cgacatccgg tcggagcgtc
    15721 ccgccgggct cctctcggtc ctctcctacg tctacatcct gccgaagcgc tccgtcgaat
    15781 cggctggaga agcggagtac ttcaaggctc cggcgggaac gggaccgtac cgcatcgcct
    15841 cgtggaagcc gggcaagtcc ctccggctcg aagccttccc gggctactgg gaggggccgc
    15901 cggagatccc ggcggccgtt ttccgcgtcg agaaggacga cgacgggatc tggaccgagg
    15961 cgaagcgcct cgcccccgcg atcctcttcg gccccacgac gacgacgtgg atccggcacc
    16021 gggaagaccc ggcgttccgg ctcgtcgagc agccgagcct ggccgtccac tacctcgcgc
    16081 tccgcgtcgg cggagggccg gagaacccgc tgtccgacgt ccgcgtccgc cgcgcgctcc
    16141 gggcctcgat cgactacgag gcgctcctgg cggcgctccc cgcgcgcgga gcctttcccg
    16201 cgagccagtt cgtccctccg gcgatcgtcg gcttcgaccc gtccctcgcg gtcccggcgt
    16261 tcgtgccggg cgccgcgcgc cggatgatcg aggagacggg cctccggcaa accaagcccc
    16321 tcgtcttcct caccgccggc ggcggcggct ccatctcccg gtacctcctc aaggccttct
    16381 ccgacgcggg cctctcggtc cgggaagagt ccgtcacccc ggaggagtac gagacccggc
    16441 tcgccgcctg cgacgccgac atcttcctca cgggctggat ctgcacgacc ggggacgcgg
    16501 gcgagctctt cgaggggaac ttctaccgga gaggctcgac gcagaacccg tgcgggtacg
    16561 gctgcgacca gatggacacc gccatcgagg agatcggccg gacgctcgac ccggccgttc
    16621 gccgggacct tctccagtcc gcgatgcgga cgctcgtcga cgacctcccg tgggtgcctc
    16681 tcctcgtcag ctacgagcgg cacgcgctca ccggcggcgt cgagtggagg acgcgggccg
    16741 acggccagct cgacctgcgc gacgtgagga tcaggtgaca ccgctccgcc gctcgctccg
    16801 gggccggttc gccctgatct tctccgccct gatgggcgcg ctcctgctcg tcgtctcggg
    16861 gac
//
LOCUS       DAOTEZ010000035        16834 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2322_length_16834_cov_6.503189, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000035 DAOTEZ010000000
VERSION     DAOTEZ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 16834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16834
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2322_length_16834_cov_6.503189"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="P5164_03090"
     CDS             complement(<1..701)
                     /locus_tag="P5164_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42911.1"
                     /translation="MNSALADLYRAHVAERQRTTEQALAETGFDRLLIHSGVPFTYFA
                     DDNDAPFRPTPHFTHWAPVEGPGHLLEVRPGKRPRLARYQPRDYWYEPPKPAPEFVAA
                     EMEIVDAESPEAVRAAIGPNGGRTAFLGGAVAEAAALGLPADAVNPKGLVARLDWERS
                     YKSAYEVESVAEAEAPAARGFRAAEAAFREGATEMEVHHAFLSAAAVMEEHLPYPTII
                     GFDERSATLHYHGKRG"
     gene            866..1090
                     /locus_tag="P5164_03095"
     CDS             866..1090
                     /locus_tag="P5164_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRY42912.1"
                     /translation="MRITSKGQVTIPVEIRERLGLLPDTEVTFEVVGPTVRLQKAPGQ
                     SRRGATVVARLRGRGQTRLTTDQILALTRG"
     gene            1094..1501
                     /locus_tag="P5164_03100"
     CDS             1094..1501
                     /locus_tag="P5164_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRY42913.1"
                     /translation="MAGFLVDSNVLLDVLTEDPSWFEWSASALARCAEEGPLAVNPLV
                     YAEVSVGFERVEDLDEALDSAGFQRLALPWAAAFLAGKCFLRYRRRGGVRSSTLPDFY
                     IGAHALVEGRTLLTRDAARYRTYFPRLALVAPK"
     gene            1658..5047
                     /locus_tag="P5164_03105"
     CDS             1658..5047
                     /locus_tag="P5164_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42914.1"
                     /translation="MSRWVRAFFLVSLLHSWVSLTADTVWKVADEGPRTPVAVEIVGD
                     DTRTIAVATGGAAEFDGTRWLPVALNTDAVLPKEREMYAVGGRIGAFSVDGTSVRLYL
                     LQGSTWSLAASEPLSNWPFWWNGRPPVPRAADRIYLPDPAFGNCETVQSCPADAAARR
                     LRSISLADGSIREEPGLPVCSGLLFGVADRLFVIAMPPPCCGACPSSAGPRTVALDDG
                     LPAYRLDGDHWTELPPWTRPLPDTFSASAAWLFERDSATTSRSLTLFTASGFSDPIPI
                     PAGQLTNNPKPIEWNGRMLLQAERLYEVRHGALLPVAPECPIPGARLFAAGSRLFAWD
                     EGWAVSALSGSAWEETSGVPEVAGGQVFFKGRTKHYAIRGNRLFRRDDSGWVGLPPSP
                     SSADLRFLYAGQFVFLEDDPVFLDQGYSGTYGAWRYDAPSGSWVDLNPPPAMRGPALV
                     HDGDLYVGNRDEAEGKLAVRHDGAWNTLETGAAVWQLREANGQLYVFGCRTSESDYDV
                     CRLDEGRLVPAFPGLEAEGMQALDVTGRNGETLVSVEDKQPPEYPASLRRVLVSADGA
                     GHRTVLRQGDLRDAGHGSYGQGLLSPLVPFGEELLLPGLSFRDGQLRAQRSPIVPSIL
                     DPKGRFAFRDSASTEYMHYRGPLLVPSARVRKNLVAAVDTTGVGGTRYRSVLLVANFS
                     ETETAVARVFAAAHWDPFLEIPLPPRAQVAIDDPKPGFLGPMAVEFEGLTDEADAWAA
                     IRVWSPSDGGTAGTAIVGTNPGVVPLNAVVVPPHAKSGSRTQAAFSASGDGGHGPVRA
                     SACPLAGREPGEPDPCVAVELLRNGAFFQAGVPPAALRSPIQFWSTCYNNWCSLLQRT
                     DDLGGYLVRNEEGTNDGAIVPFERPDVMEGRRVRFLPALVSLTSARGTYRTELTLGWR
                     STDVHPPLSLDFGVTFRSHAGAWTVPITIPARQVVEIPDAGAWLAAHGVPVDPASADG
                     TLTFTSDREEGAASLLVTAVVTARGPGASGDYGVSVPVFNEVEWASSEAIVPSLREDG
                     AFRTNLALANPESDGGPSTTLEVSVHSASDGKAVGTLPQVTLAPGHRVQLNRLLATID
                     FSGEAFAVVRRTGGEGRFVAYGVMNDNVTGDGTLFPMTRSR"
     gene            complement(5108..6331)
                     /locus_tag="P5164_03110"
     CDS             complement(5108..6331)
                     /locus_tag="P5164_03110"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRY42915.1"
                     /translation="MTTTSRPRIRFAAAFGFGLVHVAALGVLWVGLSWQAVALCLGSY
                     YLRMFAITAGFHRYFAHRTYKMNRFWQLLMAFLGQTAAQKGVLWWAAHHRHHHRFSDQ
                     PEDIHSPVQKGFWWSHMGWILADDYEETDLESIRDFAKYPELVWLDRNQFLAPMLYGL
                     GLWLAFSWTGLFWGFFLSTVLLWHGTFAINSVMHVFGRRVFETKDTSRNSFLMALVTM
                     GEGWHNNHHHFQASAAQGFLWWQADPSLWLLRLGRLLGIARDLRPVPERVVALARERA
                     AWHFTGRTFERAREAFDAAQASWDARAEQLSAAWAARRGTALASASQRLAELEAARQR
                     AAAQLERLKADYAAALSAAAAGTRLRGKRAKALAEIRVEELRAEIERTRQSLVDALER
                     LVEAAGGGEGLSSPA"
     gene            complement(6450..7217)
                     /locus_tag="P5164_03115"
     CDS             complement(6450..7217)
                     /locus_tag="P5164_03115"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRY42916.1"
                     /translation="MRGPVRRALARVHFLLACVVGALAFVGLSIGGLLYVALRAGRGS
                     APVHLARLFHWIVRTLLGWRITVEDRPRVTGSAPAILMASHRSNLDIVVYGGLYPKRT
                     VVIGKKEIAKIPVFGWFFRATGNILLDRKDLASAIASIAAAAKRVREKRVSVWVFPEG
                     HRNGSPTLLPFKKGSFHLAIAAQVPIVPIVCGPLDDVLDGHRLLVFPGTIRIRVLPPI
                     PTEGLDEEDLEELIATVRGRMQEAQDALAAETAAVRS"
     gene            complement(7214..8020)
                     /locus_tag="P5164_03120"
     CDS             complement(7214..8020)
                     /locus_tag="P5164_03120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRY42917.1"
                     /translation="MPYILRRCVYTLHFVAASIVGGALLLVVSALALAVALARGPRGH
                     ASVLAARTYSRVMQRILGWRIEAEGVERFAQAAPSVVMARHQSNLDVIVYGEVFPRGA
                     IAIGKKQLEWIPFFGWLWTATGNVLIDRRHTSAAMAAMRKAADSSRSLGASIWVAPEG
                     HRNMEREMLPFKKGVFHLALDGRFPVLPVAVSPLDTLLDARRWAVRPGTIRLRVLPPI
                     PVDDLAPGDVAALMTRVRAPFEEAQRELAASTPPPILARGATRGPEALCA"
     gene            8014..9297
                     /locus_tag="P5164_03125"
     CDS             8014..9297
                     /locus_tag="P5164_03125"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HRY42918.1"
                     /translation="MAWSPLPGSGIISAMEAGVVVVGGGAAGLLAAASAASRLRAAGS
                     AAPVVVLEAAREPGRKVLVSGGGRCNVLPGREARERFVSSSPRRLVDRFLDRFPLAAQ
                     RAFFEGLLGAPLREEESGKLFPPTNRARDVRDALVALARSAGAEVRLHSPVREVAARE
                     GGFDVRLDAGTLSASAVVLATGGRSVLAGGADAAGFDWAAAFGHSVRPLVPALVPLTG
                     SSPAHAALSGVALEAAVTARCGSETAAARGGFLFTHRGWSGPAVLDVSHVVTRALAGE
                     KPFSVVVSFGGDAAHWDETLRAASGSVLSLLRRSLPDRVASLLLAEAGVAPETPLHRM
                     TREARRAVVAALSALPLPVGGSEGYRTAEVTAGGVALEEVDPGSGASRLVPGLFLAGE
                     ILDAVGPIGGFNFQWAWATGRTAGEGAARAVLAGG"
     gene            complement(9310..9651)
                     /locus_tag="P5164_03130"
     CDS             complement(9310..9651)
                     /locus_tag="P5164_03130"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HRY42919.1"
                     /translation="MSGRVVWSSGQGRICPTCGWPANDCRCSKSLDEPVPAKVTAKLR
                     IEKKGRGGKSVTVIDALPDNGPFLEELAKAMKKACAVGGTVRPGAVELAGDVRERVRP
                     LLAARGFVVKG"
     gene            9787..10671
                     /locus_tag="P5164_03135"
     CDS             9787..10671
                     /locus_tag="P5164_03135"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRY42920.1"
                     /translation="MRTEEVEVRSGGGFSLATTLHHPEAPEEEAVVLVNAGTGIRRGF
                     YGRFAAYLASSGFPVVTWDYRGIGDSRPDSIRGLRASMQDWGREDLESVLLWLGERHP
                     GRPILVVGHSAGGQILGLAPSASRISAVLAVAAQSGWYGHWPVPRRYLMAGLWWGLMP
                     AATALCGYFPSRALGLGEDLPKGVALEWARWCRNPQYMVDANGAPLRPHFADLRAPVL
                     AFSFADDPFAPRAAVEQLLSFYTEANVTHRHVVPAEIGLRGIGHFGFFRETCREALWK
                     ECVDWLRRRGAAAERGVA"
     gene            10668..11939
                     /locus_tag="P5164_03140"
     CDS             10668..11939
                     /locus_tag="P5164_03140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY42921.1"
                     /translation="MKNVSPEPVPVPEDAQEERRGPVAVPAAAPSRARRLFLEALSEH
                     LTGTRVVFRTEDGRHEVGRGEAVAAVVRVRRERFFERALAQGNLGLGESFMDGDWEVE
                     QGTLEGLLEALLRARLDHKLAGNPGVALKVLALQLLNGLRGRQWAHVQRHYDVGDDLF
                     ELFLDRSLVYSCAYLVKEDDSIETMQEQKLDRICRKLDLAPGERLLDIGCGFGGLLIH
                     AASRFGVTGVGITTSARHAAKGMERVRAAGLDGRVTLELRDHRTVEGRFDKVVSVGMM
                     EHLPRGEYARYFERIAAVLAPEGRGLVHTIGCNAERNRHDPFIQKYVFPGSGQPKLSE
                     MAAECERHGLAILDVENMVRHYAHTARRWLDRFRERAHLLDPKRYDASFRRMWEYYLC
                     CCIAGARASDGALYQVLFARDYAAEMPLHRV"
     gene            11953..13035
                     /locus_tag="P5164_03145"
     CDS             11953..13035
                     /locus_tag="P5164_03145"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HRY42922.1"
                     /translation="MNALSVLNQRWVEAGYTRKPVAVHLLQLAAHVAFALGGTALYFL
                     VPGLAAKVLGLLVSSYGMIGISTHAHTSSHNAIFDATLPNRALVHLGFPFLCGVSALY
                     WWNKHVHVHHPTPNIIGLDDDVDLMPFFALTREEAARGGPLLKLWFRVQGIAIVPILA
                     LNCFNIQKTGWSYWVRTLRGPGAKRGAHALDLVLLLGHYAFWIGLPALFFPLGEVLLF
                     YVVRMFLLGYGMFAVFAPAHFPARASFVSAEGETNRLAYKKSKDFILLQTATTVNFRT
                     GPLGRLLCLGVEYQIEHHLFPGISHVHYPRLSVELKRFCEENGYPYRTLGWWEGILES
                     IRAFRHLKPVEPRLEELRPQAEGQAA"
     gene            13032..13919
                     /locus_tag="P5164_03150"
     CDS             13032..13919
                     /locus_tag="P5164_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY42923.1"
                     /translation="MKAERGAGALRRLGAALDRRWARSARPPNGEEIEVPGGRLRVRR
                     GGGPDGVPVVVVPDPPNVAEHYDGAFGALAAGGPVAVVEAPGFGFSAPRAGGRYRVEE
                     LSAALEELLVRFGFRGATLSLACVGAFAAVDLAARRPDLVGRLVLSQVASRREMASWV
                     KRKDVCGVIQAPLLGPVAVRAARRRIARWWYRAALPPGADPGPIAAPALAALRAGAAF
                     PLASAFQEFARRPGERLRLACPVTVLLGAADPTHAETDLGSIAEELPGARVLVFSGSG
                     HFPDLENPAEWLAALRAPA"
     gene            complement(13902..15764)
                     /locus_tag="P5164_03155"
     CDS             complement(13902..15764)
                     /locus_tag="P5164_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRY42924.1"
                     /translation="MSLSIPQILSTTRLFGGLAPEDLARIGPEFESVRVPGGETVFEH
                     GETGDCLYIVASGRIEVSLTLPDGSLRPIGEAGPGECLGEMALLTGERRSATARSRRD
                     TDLLRLSGEAFRRLLETHPRLMYGLTRRIIGHYQSVLSGRRGASAGRISTIAVATTQK
                     DGRVAEFTRRLVEALGRNGSVRHLDASALDSALGEGSAQAARDSSRNSGIVAYLNEQE
                     EAHRFVVYEAEPGPSSWTSRCLRQADRILFVAPDDGSPAPGPIEALLARQGLADVRTA
                     DLVLLHRETKPLYLGTSAWLAPRRVRAHHHVAESAPGEMERLVRSLTGEVRGLVLGGG
                     GARSFAQIGVLRALAERGVSFDVVGGTSMGAFLGAQVAMGWDPGKMLAFNEVTWTRTK
                     PLKDYTFPYLGFVTGRRFYSVARAIYGEARLEDLRVPFFCCSSNLTRARVEVHDAGPA
                     WRALGASIAVPGLAPPAFEGGDLLVDGAVLANLPVDVMRRRCSGTVVTIDVSPVEDLS
                     VDPALTMAPNSWGLLFRKLLGKKPQVPGFFELLMRSSGLASVQQVDEMQRLSDVFFHP
                     PIEKYSIFDFSLIRELERVGYEHAAQVLASPAGELLVRADAPAAGAAPASGGSA"
     gene            complement(15887..>16834)
                     /locus_tag="P5164_03160"
     CDS             complement(15887..>16834)
                     /locus_tag="P5164_03160"
                     /inference="COORDINATES: protein motif:HMM:NF014728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRY42925.1"
                     /translation="ETEARIGDLEVVPRKQVSYQGVVLPEMDLDAILRRRPDVVLVDE
                     LAHTNAPGCRNEKRWKDVEEVLAAGISVMAAVNVQHLEGVQDVVGSVTGIEVRERLPD
                     RIVRDADALVVVDLPVEELRERIRIGKVYAPEKAEQALSSFFTEEKLTRLRELTLFQT
                     ADHIEADAHHDSGAFEAASTQARVAAAIPLEPETGRRLILRASRVAGRMNTRWFALHV
                     RRSRERPERLSARQHRALTENVELAMTLGATVVVREAEDVAAEILRFVKAENVGIVIV
                     GPPSRRGLAARLFPGTVDRLLAGTDGFSLLVADAGEDER"
BASE COUNT         2242 a         6021 c         6113 g         2458 t
ORIGIN      
        1 ccgcgcttgc cgtggtagtg gagcgtcgcg gagcgctcgt cgaacccgat gatcgtcggg
       61 taggggaggt gctcctccat cacggcggcg gcggagagga aggcgtggtg cacctccatc
      121 tccgtcgcgc cctcccggaa ggctgcctcg gcggcgcgga agccgcgcgc ggcgggggcc
      181 tccgcctcgg cgacggactc cacctcgtag gcgctcttgt acgaccgctc ccagtccagc
      241 cgggcgacga gccccttcgg gttgacggcg tcggccggga gacccagcgc ggcggcctcg
      301 gcgacggcgc ctccgaggaa ggcggtgcgg ccgccgttcg ggccgatcgc cgcgcggacc
      361 gcctcggggc tctccgcgtc gacgatctcc atctccgcgg cgacgaactc cggcgcgggc
      421 ttcggcggct cgtaccagta gtcgcgcggc tggtagcggg cgagccgcgg cctcttcccg
      481 gggcggacct cgaggaggtg ccccggcccc tcgaccggcg cccagtgcgt gaagtggggc
      541 gtcgggcgga acggcgcgtc gttgtcgtcg gcgaagtagg tgaaggggac gccggagtgg
      601 atcaggaggc ggtcgaagcc ggtctcggcc agggcctgct ccgtcgtccg ctgccgctcg
      661 gcgacgtggg cgcgatagag gtccgcgagg gcgctgttca tggaggcgga tgttagcgaa
      721 ccggaggccg gccccgagct cggacttcgg ccggagctct tcgggacggt gcgcgcgcgg
      781 gcgcgatgtc tccgacgccc cggatggtcc tcccacggag acttgaagta ggaatcggaa
      841 gagtatgatt ccaactgagg tcgtcatgcg catcacgtcc aagggccagg tcacgattcc
      901 ggtcgagatc cgcgagcgcc tcggccttct gcccgacacg gaggtcacgt tcgaggtcgt
      961 cggccccacg gtccgactgc agaaagcccc ggggcagagc cgacggggag cgaccgtcgt
     1021 cgcccggctg aggggccggg ggcagacccg cctcacgacc gatcagatcc tcgccctgac
     1081 ccggggctga ggcatggccg ggttcctcgt cgacagcaac gtcctcctcg acgtcctgac
     1141 ggaggaccct tcgtggttcg agtggtctgc atcggccctc gcccgctgcg ccgaggaggg
     1201 gccgctcgcc gtcaatcccc tcgtctacgc ggaggtctcg gtcggattcg agcgggtcga
     1261 ggacctggac gaggcgctcg actcggccgg atttcagagg ctcgcgctgc cgtgggcggc
     1321 cgcgttcctc gcggggaagt gcttccttcg ataccgccgt cgcggcggcg tccgctcctc
     1381 gacgcttccc gacttctaca tcggcgccca cgccctggtc gaaggacgga cgctcctgac
     1441 gcgggacgcc gcgcgctacc ggacgtactt cccgcgcctc gcgctcgtcg cgccgaagta
     1501 gaggacggca agctccccga ggagtctccc cgaggggtca ggtctaccat ctacactcta
     1561 cgggttcccg tagaatgtag atggtagacc tgacccccct tcctttgccc ggccgtagga
     1621 ttcagcggtc ggggggcact tcctcaagga ggcgcggatg tcgcgatggg tgcgggcctt
     1681 ctttctcgtt tcgctccttc actcctgggt ctcgctgaca gccgacaccg tctggaaggt
     1741 cgccgacgag ggcccgcgga cacccgtcgc cgtcgagatc gtcggggacg atacgaggac
     1801 gatcgccgtc gcgaccggcg gggctgcgga attcgacggg acgcgctggc ttccggtcgc
     1861 cctgaacacc gacgcggtcc tgccgaagga gcgggagatg tacgcggtcg gtggccggat
     1921 cggggcattc tccgtggacg gcacctcggt gcgcctctac ctcctgcagg gaagcacctg
     1981 gtccctggca gcgtccgagc ccctctcgaa ttggccgttc tggtggaacg gccggcctcc
     2041 cgtgccgcgc gccgccgatc gcatctacct gccggatccc gcgttcggga actgcgagac
     2101 cgtacagagt tgcccggccg acgcggccgc gcgtcgcctc cggtcgatct cgctcgccga
     2161 cggctccatc cgggaagagc ccggtctccc tgtctgctcg ggtctcctct tcggcgtcgc
     2221 cgatcgactc ttcgtcatcg ccatgccgcc gccctgctgc ggagcctgtc cgagcagtgc
     2281 ggggccgcgc acggtggcgc tcgacgacgg tctccccgcc tatcgcctcg acggcgacca
     2341 ctggacggag ctcccaccgt ggacgcgacc gcttcccgac accttctctg cgagcgcggc
     2401 ctggctgttc gagagggact cggcgacgac ttccaggtcg cttaccctct tcaccgcgag
     2461 cgggttctcg gatccgatcc cgatccccgc cggtcagctg acgaacaacc cgaaaccgat
     2521 cgagtggaac ggtcggatgc tgctgcaggc cgagaggctc tacgaggtgc gccacggcgc
     2581 cttgctcccg gtcgccccgg aatgcccgat accgggcgcg aggctcttcg cagccgggag
     2641 tcggctgttc gcctgggacg agggctgggc ggtctccgcg ctctcgggct cggcgtggga
     2701 ggagacctcc ggcgttcccg aggtcgccgg cgggcaggtc ttcttcaagg gacggacgaa
     2761 gcactacgcg atccggggga atcgtctctt ccggcgggac gactccggct gggtcggcct
     2821 tcctccgtcg ccctcgtcgg ccgacctccg tttcctgtac gccggtcagt tcgtcttcct
     2881 ggaggacgac ccggtcttcc tcgatcaggg ctattcgggc acctatggcg cgtggcgata
     2941 cgacgccccg tccggctcct gggtggacct caaccccccg cccgcgatgc gcggcccggc
     3001 cctcgtccac gacggggacc tgtacgtcgg gaatcgcgac gaggcggagg ggaagctcgc
     3061 ggtccggcac gacggggcgt ggaacacgct ggagaccggt gccgccgttt ggcagcttcg
     3121 ggaagcgaac gggcagctct acgtcttcgg atgccggacg agcgagtccg actacgacgt
     3181 ctgccggctg gacgagggcc ggctggtccc cgccttcccg gggctcgagg ctgagggtat
     3241 gcaggcgctc gatgtcactg ggaggaacgg agagactctc gtctccgtcg aggacaagca
     3301 gcctcccgag tacccggcgt ccttgcgaag ggtgctcgtc tcggcggatg gcgcgggcca
     3361 ccgaactgtc ctccgccagg gggacctccg ggacgcgggg cacggcagct acggccaggg
     3421 cctgctctcg ccgctcgtcc ccttcggcga ggagctcctc ctgccgggcc tctccttccg
     3481 tgacggtcag ctccgcgcgc agcggagccc gatcgtcccg agcatcctcg atccgaaggg
     3541 gcggttcgcg ttccgggaca gcgcctcgac ggagtacatg cactaccgcg gacctctgct
     3601 cgtcccgtcc gcgagggtcc gcaagaacct cgtcgcggcg gtcgacacga cgggcgtggg
     3661 cgggacccgg tatcggagcg ttcttctcgt ggcgaatttc tcggagaccg agacggccgt
     3721 cgcacgcgtc ttcgcggcgg cccactggga cccgttcctc gagatacctc tgcctccgcg
     3781 agcccaggtg gcgatcgacg atccgaagcc gggattcctc gggccgatgg cggtggaatt
     3841 cgagggactt acggacgagg ccgacgcctg ggccgcgatt cgggtctgga gcccgtcgga
     3901 cggagggacg gccgggacgg cgatcgtggg gacgaacccg ggagtcgtcc cgctgaacgc
     3961 cgtcgtcgtc ccgcctcacg ctaagagcgg cagccggacc caggcggcct tctcggcttc
     4021 gggcgacggc ggtcacggac cggtacgcgc gagcgcttgt ccattggcag ggcgggagcc
     4081 gggcgagccc gacccgtgcg tcgcggtgga gcttcttcgg aacggtgcct tcttccaggc
     4141 cggcgtccca ccagcggctc tccgctcgcc catccagttc tggagcacct gctacaacaa
     4201 ctggtgctct ctgcttcagc gaacggacga cctcgggggg tatctcgtcc ggaacgagga
     4261 agggacgaac gacggcgcga tcgtgccgtt cgagcgtccc gacgtcatgg aggggcgaag
     4321 agtccgattt ctccccgccc tcgtctcgct cacttcggcc cggggcacct atcggaccga
     4381 gctgacgctc gggtggcgat ccacggacgt gcatcctccg ctgagcctcg atttcggggt
     4441 gacgttccgg agccatgccg gggcgtggac ggtcccgatc acgatccctg cgcggcaggt
     4501 cgtcgaaatc ccggatgcgg gggcgtggct ggctgctcac ggcgtcccgg tcgatccggc
     4561 gagcgccgat ggcacgctga cgttcacctc cgatcgcgag gagggcgcgg cgagcctact
     4621 cgtgacggcc gtcgtgacgg cccgaggccc gggcgcctcc ggcgactacg gtgtgagcgt
     4681 ccccgtgttc aacgaggtcg agtgggcgtc gagtgaagcg atcgtgccta gcctcaggga
     4741 agacggggcc ttccgaacga acctcgcgct ggccaacccg gaatcggacg gcggcccctc
     4801 gaccacgctc gaggtctcgg tccacagtgc gtcggacggg aaggccgtcg ggaccctccc
     4861 gcaagtgacg ctcgctccgg gacatcgcgt ccagctgaac cggctcctcg cgacgatcga
     4921 cttctcgggc gaagccttcg cggtggtccg gcgaacgggg ggggagggac ggttcgtcgc
     4981 ctacggggtg atgaacgaca acgtcacagg ggacggcacg ctctttccaa tgactcggtc
     5041 gcgataggag cgggagctcc tccgcgagga cgcgccacga ggcggccggg cggaccccgg
     5101 tccgacctca ggccggcgag gagagcccct cgccgccgcc ggccgcctcg acgagccgct
     5161 cgagcgcgtc gacgaggctc tggcgggtcc gctcgatctc ggcgcggagc tcctcgaccc
     5221 ggatctcggc gagcgccttg gcgcgctttc cgcgcaggcg cgtgccggcg gccgcggccg
     5281 agagggctgc ggcgtagtcg gccttcagcc tttcgagctg cgcggcggcg cgctgccgcg
     5341 cggcctcgag ctcggcgagc ctctgcgagg ccgaggcgag cgcggtcccg cgccgggcgg
     5401 cccaggccgc ggagagctgc tcggcgcgcg cgtcccacga cgcctgcgcg gcgtcgaacg
     5461 cttcgcgggc ccgttcgaac gtccgcccgg tgaagtgcca ggcggcccgc tcgcgagcga
     5521 gggcgacgac ccgctccggc accgggcgaa ggtcccgcgc gattccgagg agccggccga
     5581 gccggaggag ccagaggctc gggtcggcct gccaccagag gaagccctgc gcggcggagg
     5641 cctggaagtg gtggtggttg ttgtgccacc cctcgcccat cgtcacgagc gccatcagga
     5701 agctgttgcg gctcgtgtcc ttcgtctcga agacgcgtcg tccgaagacg tgcatcacgc
     5761 tgttgatggc gaacgtcccg tgccagagga ggaccgtcga gaggaagaag ccccagaaga
     5821 ggccggtcca cgagaacgcg agccagagcc cgagcccgta gagcatcggc gcgaggaact
     5881 ggttgcggtc gagccagacg agctcggggt acttcgcgaa gtcgcggatc gactcgaggt
     5941 cggtctcctc gtagtcgtcg gcgaggatcc accccatgtg gctccaccag aatcccttct
     6001 gcacgggcga gtggatgtcc tcgggctggt cggagaagcg gtggtggtgc cggtggtgcg
     6061 cggcccacca gaggactccc ttctgcgccg ccgtctgccc gaggaacgcc atcaggagct
     6121 gccagaagcg gttcatcttg taggtccggt gggcgaagta gcggtggaag cccgcggtga
     6181 tcgcgaacat ccggaggtag tacgacccga ggcagagcgc gacggcctgc caggagagcc
     6241 cgacccacag gacgccgaga gcggcgacgt ggacgaggcc gaagccgaag gcggcggcga
     6301 agcggatgcg ggggcgcgag gtcgtcgtca tcgggcgtac gtccttcgga gaacgagagg
     6361 gctttcgcgg ctcgagccgc gggcgctcca tcttacggag ttgcgaaccg agcgcaacga
     6421 cgggtcggtg ccgcccgggc ggggcgcggt caggagcgga cggccgccgt ctccgccgcc
     6481 agggcgtcct gcgcctcctg catccggccg cggacggtcg cgatcagctc ctcgaggtcc
     6541 tcctcgtcga gcccctcggt cgggatcggc ggaaggacgc ggatccggat cgtcccgggg
     6601 aacacgagga ggcggtggcc gtcgaggacg tcgtcgagcg gcccgcagac gatcggcacg
     6661 atcggcacct gcgcggcgat cgcgaggtgg aacgagccct tcttgaacgg caggagcgtc
     6721 ggcgagccgt tccggtgccc ttccgggaag acccagacgg agacccgctt ctcccggaca
     6781 cgcttcgccg ccgcggcgat cgaggcgatc gcgctcgcca ggtctttccg gtcgaggagg
     6841 atgttgccgg tcgcgcggaa gaaccagccg aagacgggga tcttcgcgat ctccttcttg
     6901 ccgatgacga ccgtccgctt cgggtagagg ccgccgtaga cgacgatgtc gaggttcgac
     6961 cggtgagagg ccatcaggat cgccggggcc gagccggtga cgcgcggccg gtcctcgacc
     7021 gtgatccgcc atccgaggag cgtccggacg atccagtgga agagccgggc gaggtgaacg
     7081 ggagcgctgc cgcgcccggc gcgaagcgcg acgtagagga ggcctccgat cgagagcccg
     7141 acgaacgcga gcgcgccgac gacgcacgcg aggaggaagt ggacccgcgc gagcgcgcgg
     7201 cgaacgggcc ctctcacgcg cagagggcct cgggcccgcg cgtcgcgccg cgcgcgagga
     7261 tcggcggagg cgtcgacgcc gcgagctccc gctgcgcctc ctcgaacgga gcccgcacgc
     7321 gcgtcatcag ggccgcgacg tcgcccggcg cgaggtcgtc gaccgggatc ggcgggagga
     7381 cgcgcaggcg gatcgtcccg gggcgaaccg cccagcggcg ggcgtcgagg agcgtgtcga
     7441 gggggctgac ggccaccggg aggacgggga agcgcccgtc gagggcgagg tggaagaccc
     7501 ccttcttgaa cgggagcatc tccctctcca tgttgcggtg cccctccggc gcgacccaga
     7561 tcgaggctcc caggctccgg ctcgagtcgg cggccttccg catcgccgcc atcgcggccg
     7621 acgtgtgccg gcggtcgatc aggacgttcc ccgtcgccgt ccagagccag ccgaagaagg
     7681 ggatccactc cagctgcttc ttgccgatgg cgatggcgcc gcgcgggaag acctccccgt
     7741 agacgatgac gtcgaggttc gactggtgac gggccatgac gacggaggga gctgcctggg
     7801 cgaagcgctc gacgccctcg gcctcgatgc gccatcccag gatcctctgc atgacgcggg
     7861 aataggtccg ggcggcgagg acggaggcgt ggccccgcgg accgcgtgcg agggcgaccg
     7921 cgagcgccag cgccgagacg acgaggagca gggccccccc gacgatcgac gcggcgacga
     7981 agtggagcgt gtaaacgcag cggcggagga tgtatggcat ggagcccgct tccgggaagc
     8041 ggcattatct ccgcgatgga ggcgggcgtc gtcgtcgtcg gcggaggggc cgcggggctc
     8101 ctcgcggcgg cgtcggcggc gagccggctc cgcgcggcgg ggagcgccgc ccccgtcgtc
     8161 gtcctcgaag ccgcccgcga gccggggcgc aaggtcctcg tctccggggg cgggcgctgc
     8221 aacgtcctcc cggggcgcga ggcgcgggag cgcttcgtct cctcttcccc gcgccggctc
     8281 gtcgaccgct tcctcgaccg cttcccgctc gccgcccagc gcgccttctt cgaggggctc
     8341 ctcggcgcgc cgctccgcga ggaggagagc gggaagctct tcccgccgac gaaccgggcg
     8401 cgcgacgtgc gcgacgcgct cgtcgccctc gcgcggagcg cgggggcgga agttcgcttg
     8461 cactctcccg tccgggaggt cgcggcccgg gagggcggct tcgacgtccg cctcgacgcg
     8521 gggacgctct ccgcgtccgc cgtcgtcctc gccacgggcg ggcgctccgt cctcgcgggt
     8581 ggcgccgatg cggcgggctt cgactgggcg gcggcgttcg gccactcggt ccggccgctc
     8641 gtccccgccc tcgtcccgct cacgggctcc tcgcccgcgc acgccgccct ctccggcgtc
     8701 gcgctcgagg cggcggtgac cgcgcgctgc ggaagcgaga cggccgcggc gcgcggcggc
     8761 ttcctcttca cccaccgggg ctggagcggc ccggccgtcc tcgacgtctc ccacgtcgtg
     8821 acgcgcgccc tcgccgggga gaagccgttc tccgtcgtgg tctccttcgg cggcgacgcg
     8881 gcgcactggg acgagacgct ccgagcggcc tcggggagcg tcctctccct cctccgccgc
     8941 tcgctccccg accgcgtcgc gtcgctcctc ctcgccgaag ccggcgtcgc gcccgagacg
     9001 ccgctccacc ggatgacgcg cgaggcgaga cgtgccgtcg tcgcggcgct ctcggccctt
     9061 ccgctcccgg tcggcgggag cgagggctac cggacggccg aggtgaccgc cggcggcgtc
     9121 gcgctcgagg aggtcgaccc ggggagcggc gcgagccgcc tcgtcccggg actcttcctc
     9181 gcgggggaga tcctcgacgc cgtcggcccg atcggcggct tcaacttcca gtgggcctgg
     9241 gcgacggggc gaacggccgg cgagggggcg gcgcgggcgg ttctcgcggg cggctgagcg
     9301 cgctcgcgct cagcccttga cgacgaagcc gcgcgccgcg agcagcggcc gcacgcgctc
     9361 ccggacgtcg cccgcgagct cgacggcgcc cggccggacc gtcccgccga ccgcgcacgc
     9421 cttcttcatt gccttcgcga gctcttcgag gaacggcccg ttgtcgggga gcgcgtcgat
     9481 gacggtcacg ctcttcccgc cgcggccctt cttctcgatc cggagcttcg ccgtcacctt
     9541 cgcggggacc ggctcgtcga ggctcttcga gcagcggcag tcgttggccg gccagccgca
     9601 cgtcgggcag atccgaccct gaccgctcga ccagacgacg cgtccgctca cgggcagatc
     9661 ttctcagacg cctcgggtcc ggccttcggt cgtgactggc ctcacgggaa gttgagatag
     9721 acttgcaact aaaccgacgg gacaccagat tgacgtttcg aggcaccccc tcggatgacg
     9781 ttcggcatgc gcacggaaga ggtcgaggtc cgctccggag gcggcttttc gctggcgacg
     9841 accctccatc acccggaggc gccggaggag gaggccgtcg tcctcgtgaa cgccggaacc
     9901 gggatacgcc gcggcttcta cgggcggttc gcggcctatc tcgcgtcgag cggcttcccg
     9961 gtcgtgacgt gggactaccg cgggatcggc gactcgcgtc ccgactcgat ccgcgggctt
    10021 cgggcctcga tgcaggactg gggtcgcgag gacctcgagt ccgtcctcct ctggctcggc
    10081 gagaggcatc cgggccgtcc gatcctcgtc gtcggccaca gcgccggcgg acagatcctg
    10141 ggcctcgccc cgtcggcctc gcggatctcg gcggtcctcg ccgtcgccgc gcagagcggc
    10201 tggtacgggc actggcccgt gccgcgccgg tacctgatgg ccggactctg gtgggggctc
    10261 atgccggcgg cgacggcgct ctgcggctac ttcccctctc gggcgctcgg cctcggcgaa
    10321 gacctgccga agggggtcgc cctcgagtgg gctcgctggt gccggaaccc gcagtacatg
    10381 gtcgacgcga acggggcccc cctccggcct catttcgccg acctgcgcgc tccggtcctc
    10441 gccttcagct tcgccgacga cccgttcgcc ccgcgcgcgg ccgtcgagca gctgctctcc
    10501 ttctacacgg aagcgaacgt cacccaccgc cacgtcgtcc cggcggagat cggcctccgg
    10561 ggcatcggcc atttcggctt cttccgagag acctgccgcg aggcgctctg gaaggagtgc
    10621 gtcgactggc tgcggcgccg aggcgccgca gccgaaagag gagtcgcatg aagaacgtct
    10681 ctccggagcc cgtgccggtc cccgaggacg cgcaagagga gcgccgaggg ccggtcgccg
    10741 ttcccgccgc cgcaccgtcg cgggcgcgcc ggctcttcct cgaggcgctc tcggagcacc
    10801 tgaccggcac gcgggtcgtc ttccgaacgg aggacggccg gcacgaggtc ggccggggcg
    10861 aggcggtcgc cgccgtcgtg agggtccgcc gcgagcgctt cttcgagagg gctctcgccc
    10921 aggggaacct gggcttgggc gagtcgttca tggacggcga ctgggaggtc gagcagggga
    10981 cgctcgaggg gctcctcgag gcgctcctcc gggcccggct cgaccacaag ctcgccggca
    11041 atccgggcgt cgcgctgaag gtcctcgcgc tccagctcct gaacggcctg cggggccggc
    11101 agtgggcgca cgtccagcgc cactacgacg tcggcgacga cctcttcgag ctcttcctcg
    11161 accgctccct cgtctactcc tgcgcctacc tggtgaagga ggacgactcg atcgagacga
    11221 tgcaggagca gaagctcgac cggatctgcc ggaagctcga cctggcgcca ggcgagcgcc
    11281 tcctcgacat cggctgcggc ttcggcggcc tcctgatcca cgccgcttcg cgcttcggcg
    11341 tcacgggcgt cgggatcacg acgagcgcgc gccacgcggc gaaggggatg gagcgggtcc
    11401 gcgcggcggg gctcgacggg cgcgtcaccc tcgagctgcg cgaccaccgg acggtcgagg
    11461 ggcgcttcga caaggtcgtc agcgtcggga tgatggagca cctgccgcgg ggcgagtacg
    11521 cgcgctactt cgagcggatc gcggcggtcc tcgcgcccga ggggcgcggc ctcgtccaca
    11581 cgatcggctg caacgccgag aggaaccgcc acgacccctt catccagaag tacgtcttcc
    11641 ccggctcggg gcagccgaag ctctcggaga tggcggccga gtgcgagcgg cacggcctcg
    11701 cgatcctgga cgtcgagaac atggtgcggc actacgcgca cacggcccgg cgctggctcg
    11761 accgcttccg cgagcgggcg cacctcctcg acccgaagcg ctacgacgcc tcgttccgga
    11821 ggatgtggga gtactacctc tgctgctgca tcgccggggc gcgcgcctcg gacggcgcgc
    11881 tctaccaggt gctcttcgcc cgcgactacg cggccgagat gcccctccac cgcgtctgag
    11941 gaggccgaga ccgtgaacgc cctctccgtc ctgaaccagc ggtgggtcga ggccggctac
    12001 acgcgcaagc ccgtcgccgt ccacctcctc cagctcgcgg cccacgtcgc cttcgcgctc
    12061 gggggcaccg cgctctactt cctcgtgccg ggtctcgcgg cgaaggtgct cggcctcctc
    12121 gtctcctcct acgggatgat cggcatctcg acgcacgcgc acacctcctc gcacaacgcg
    12181 atcttcgacg cgactctgcc gaatcgggcg ctcgtccacc tcggcttccc gttcctctgc
    12241 ggcgtctcgg cgctctactg gtggaacaag cacgtccatg tccaccaccc gacgcccaac
    12301 atcatcggcc tcgacgacga cgtcgacctg atgcccttct tcgccctcac gcgggaggag
    12361 gccgcccgcg gcggaccgct gctgaagctc tggttccggg tccaggggat cgcgatcgtc
    12421 ccgatcctcg ccctcaactg cttcaacatc cagaagaccg gctggtcgta ctgggtccgg
    12481 accctccgcg gtcccggggc gaagcgcggg gcgcatgcgc tcgatctcgt cctcctcctc
    12541 gggcactacg ccttctggat cggcctcccg gcgctcttct tcccgctcgg cgaggtcctc
    12601 ctcttctacg tcgtccggat gttcctgctc ggctacggga tgttcgccgt cttcgccccg
    12661 gcccacttcc cggcgcgggc cagcttcgtc tcggcggagg gggaaacgaa ccggctcgcc
    12721 tacaagaagt cgaaggactt catcctcctc cagacggcga cgacggtgaa cttccggacc
    12781 ggtcccctcg gccgcctcct ctgcctcggc gtggagtacc agatcgagca ccacctcttc
    12841 ccggggatct cgcacgtcca ctacccgcgg ctctcggtgg agctgaagcg cttctgcgag
    12901 gagaacggct acccgtatcg gacgctcggc tggtgggagg ggatcctcga gtcgatccgc
    12961 gccttccggc acctgaagcc ggtcgagccg cgcctcgagg agcttcgccc gcaggcggag
    13021 gggcaggcgg cgtgaaggcg gagcgcggcg caggggcgct ccggcgcctc ggcgccgcgc
    13081 tcgaccgccg ttgggcgcga tcggcgcgcc cgccgaacgg agaggagatc gaggtcccgg
    13141 gcgggcgcct ccgcgttcgc cgaggcggcg gacccgacgg cgtccccgtc gtcgtcgtcc
    13201 ccgacccgcc gaacgtcgcc gagcactacg acggcgcctt cggcgccctc gcggccggcg
    13261 gccccgtggc ggtcgtcgag gcccccggct tcggcttctc ggcgccccgg gccggagggc
    13321 gctaccgcgt cgaggagctt tcggccgcgc tcgaggagct cctcgtgcgc ttcggattcc
    13381 ggggcgcgac cctctcgctc gcctgcgtcg gcgcgttcgc ggcggtggac ctcgcggcga
    13441 ggcggccgga cctcgtgggc cgcctcgtcc tctcgcaggt cgcgtcgcgg cgcgagatgg
    13501 cttcgtgggt gaagcgcaag gacgtctgcg gggtcatcca ggcgccgctc ctcggcccgg
    13561 tcgcggtccg ggcggcgcgc cggcgaatcg cccggtggtg gtaccgcgcc gccctgccgc
    13621 ccggggccga ccccggcccg atcgccgcac ctgcgctcgc cgcgctccgg gccggggcgg
    13681 cgttcccgct cgcatcggcg ttccaggagt tcgcgcgccg tcccggcgaa cgccttcggc
    13741 tcgcctgccc cgtcaccgtc ctcctcggcg cggccgaccc gacacatgcg gagaccgatc
    13801 tcggatcgat cgcggaggag ctcccgggcg cgcgcgtcct cgtcttctcc ggctcgggcc
    13861 acttccccga tctcgagaac cccgcggagt ggctcgccgc cctacgcgct cccgcctgag
    13921 gcgggtgcgg cgcccgccgc gggcgcgtcc gcccgcacga gaagctcacc ggccggagag
    13981 gcgagcacct gggccgcgtg ctcgtagccg acccgctcga gctcccggat gagggagaag
    14041 tcgaagatgg agtacttctc gatggggggg tggaagaaga cgtccgacag gcgctgcatc
    14101 tcatccacct gctggacgct cgcgaggccc gaggagcgca tgaggagctc gaagaagccg
    14161 ggcacctgag gcttcttccc gaggagcttg cggaagagaa gcccccagga attcggcgcc
    14221 atcgtcagcg cggggtcgac gctcaggtcc tccacggggc tgacgtcgat cgtgacgacg
    14281 gtcccggagc agcggcggcg catcacgtcg acggggaggt tcgcgaggac ggcgccgtcg
    14341 acgaggaggt cgcccccctc gaaggccggc ggcgcgaggc ccggcaccgc gatgctcgcg
    14401 ccgagggccc tccaggcggg cccggcgtcg tgcacctcga cgcgcgcccg cgtcaggttg
    14461 ctcgagcagc agaagaacgg gacccggagg tcttcgaggc gcgcctcgcc gtagatcgcg
    14521 cgggccacgg agtagaagcg cctcccggtg acgaagccga ggtacgggaa cgtgtagtcc
    14581 ttgaggggct tcgtccgggt ccacgtcacc tcgttgaacg cgagcatctt ccccgggtcc
    14641 caccccatcg cgacctgcgc gccgaggaag gcgcccatgc tcgtcccgcc cacgacgtcg
    14701 aacgagacgc cccgttccgc gagcgcccgg aggacgccga tctgggcgaa ggagcgggct
    14761 ccgccgccgc cgaggacgag gccgcgcacc tcgcccgtca gcgaccggac gaggcgctcc
    14821 atctcgcccg gcgcgctctc ggcgacgtgg tgatgggccc ggacgcgccg aggcgcgagc
    14881 cacgcgctcg tcccgaggta gagcggcttc gtctcgcggt ggaggaggac gaggtcggcg
    14941 gtcctcacgt cggcgagacc ctggcgggcg agcagcgcct cgatcggccc gggagcgggg
    15001 ctcccgtcgt cgggcgcgac gaagaggatc cggtcggcct ggcggaggca gcggctcgtc
    15061 caggacgagg gaccgggctc cgcttcgtag acgacgaagc ggtgcgcctc ctcctgctcg
    15121 ttcaggtacg ccacgattcc ggagttgcgc gaggagtcgc gggcggcctg ggcggagccc
    15181 tcgccgagcg cggagtcgag cgcggacgca tcgaggtgcc ggacgctccc gttccgtccg
    15241 agagcctcga cgagccgccg agtgaactcc gcgacccgac cgtccttctg cgtcgtggct
    15301 acggcgatcg tcgagatccg tccggcggag gcgccgcgcc ggccgctgag gaccgactgg
    15361 tagtgcccga tgatccgacg cgtcaggccg tacatgaggc gcggatgcgt ctcgaggagc
    15421 cggcggaacg cctcacccga caggcggagg aggtcggtgt cgcgacgcga tcgggccgtt
    15481 gcgctccgcc gctcgcccgt cagcagcgcc atctcgccga ggcactcccc ggggccggcc
    15541 tcgccgatcg ggcgaagcga gccgtcgggg agcgtcagcg acacctcgat ccggccgctc
    15601 gcgacgatgt agaggcagtc gcccgtctcc ccgtgctcga agacggtctc gcctccgggg
    15661 acccggaccg actcgaactc cgggccgatg cgcgcgaggt cctccggggc gaggcccccg
    15721 aagaggcgcg tcgtcgacag gatctgggga atcgacaggc tcacaagcac cctccgtcgg
    15781 cggcgttcgg cgttccggcg atttttgggg gcggatgcta gcaagaacgg ggcggggagc
    15841 gtgcgcgacg ctgcggcggc gaggacgtgt cgacggagtc gtcgcgtcag cgctcgtctt
    15901 ccccggcgtc tgcgacgagg aggctgaagc cgtcggtccc ggcgaggagg cggtcgaccg
    15961 tcccggggaa gaggcgggcc gcgagtcccc ggcgcgaggg ggggccgacg atgacgatgc
    16021 cgacgttctc ggccttcacg aaccgcagga tctcggcggc cacgtcctcg gcctccctca
    16081 cgacgacggt cgcgccgagc gtcatcgcga gctcgacgtt ctccgtcagg gccctgtgct
    16141 gccgggcgga gaggcgctcg gggcgctcgc gcgagcggcg cacgtggagg gcgaaccacc
    16201 gcgtgttcat cctgccggcg acgcgcgagg cgcggaggat cagcctccgc cccgtctcgg
    16261 gctcgagcgg gatcgccgcc gcgacccgcg cctgcgtcga ggcggcctcg aacgcccccg
    16321 agtcgtggtg ggcgtcggcc tcgatgtggt cggccgtctg gaagagcgtc agctcgcgca
    16381 ggcgcgtcag cttctcctcg gtgaagaagc tcgagagcgc ctgctcggcc ttctccggcg
    16441 cgtagacctt cccgatccgg atccgctcgc ggagctcctc cacggggagg tcgacgacga
    16501 cgagggcgtc ggcgtcccga acgatccggt cggggaggcg ttcgcgcacc tcgatcccgg
    16561 tgaccgaccc gacgacgtcc tgcacccctt cgaggtgctg gacgttcacg gcggccatca
    16621 ccgagatccc ggccgcgagg acctcctcga cgtccttcca ccgcttctcg ttccggcagc
    16681 cgggcgcgtt cgtgtgggcc agctcgtcga cgaggacgac gtcgggcctc cgtcggagga
    16741 tggcgtcgag gtccatctcc ggcaggacga ccccctggta gctcacctgc ttgcgcggga
    16801 cgacctcgag gtcgccgatc cgcgcctcgg tctc
//
LOCUS       DAOTEZ010000036        16587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2367_length_16587_cov_6.461892, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000036 DAOTEZ010000000
VERSION     DAOTEZ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 16587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16587
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2367_length_16587_cov_6.461892"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..2475)
                     /locus_tag="P5164_03165"
     CDS             complement(217..2475)
                     /locus_tag="P5164_03165"
                     /inference="COORDINATES: protein motif:HMM:NF023726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3631 domain-containing protein"
                     /protein_id="HRY42926.1"
                     /translation="MTPFKTVVSALEAHGSRGKGSAWQCPAHEDRTASLSVSQGRDGR
                     ALVKCHAGCDLEAILGALGLAVADISPEREERRGSKRIKAVYRYPDETGALLYEVVRF
                     EPKDFRQRRPDPKSKDGFAWNLNGTRRVLYRLPEVLEAVRSGLPLYYVEGEKDADNLR
                     AVGLRATTHAGGAKAWRPADYIEPLRGARVVLLPDDDPPGRDLGRTIAKDLVGVAAEV
                     RVLNLGRPGVKDVSDWLAAGGTADELLRLAAEAPTAESLASVDGAEVLDAVVRFVRRY
                     VVVTPEQTHIIALWVLHTWAFDASDQTVYLAILSAEKRSGKTRLLEILGVVTRAPWHA
                     VQPSEAVLYRKIEKDRPTLLLDETDALFSQRGDSRAEAVRALLNAGSRRGVTVPRVLD
                     AKSDKLIDFNVYCPKALAGIGTLPDTVGDRSVPIEMKRKRRSDTAERFRLRDVEKEAR
                     ELRARLEAWATARVPQLRSARPALPRELGDRAADGAEPLLAIADLAGGPWPERARRSI
                     VALAGERTEEDESDGVQLLADVRRVLTLLVRTDGTIRTEKLLEELRALPESRWKRFGP
                     RREEVDALDLARLLRPYGIRPTRIRDGDAVVRGYMVSAFADAFERYLSEPPQPEGSAT
                     PATPLQSDNHAGQNVAPYPLQHPLQTPAATPKSGTCSGSCSGSPATVSSDNHAGCSGV
                     APVAGQETHADFTYDEDGLPRAEWRPGTCPTVEDEEAPEPEAVVRCGRPLHPGAEVCR
                     EHADPAFARGAA"
     gene            complement(3637..4509)
                     /locus_tag="P5164_03170"
     CDS             complement(3637..4509)
                     /locus_tag="P5164_03170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY42927.1"
                     /translation="MSSAESPAAPWPKLDPEKRSADVRAFFDRRARVYDRVADRPYWD
                     FSDRVLLELLRRTILTGLGDGDGLRLLDAGGGTGRWALHLLRELPRATAVLADVSPGM
                     LAAARRKAVRAGLLGRLSLLAHDLHEPLPRRLGRFDLVLCFHNVASLVADPAALFARL
                     ARAAVGRGRVAFVLPSLWQAAAKVLRDGRPSELRRLAASASVRYAPGVPEVLVFTPAI
                     ARALLQRAGCTEVSVRGFPVTVHPEGPDEALPAHLASPRLRRRLVPLEAALCLSEEAA
                     ARGNNLLAIGRTRR"
     gene            4551..5309
                     /locus_tag="P5164_03175"
     CDS             4551..5309
                     /locus_tag="P5164_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002757538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HRY42928.1"
                     /translation="MTKARPYGTHPDVPDHRDLRWEAPRHVRRALPAAVDLRPGCPPV
                     EDQRPLNACSAHAIGAALWYDERRQGEEAPLPSRLFLYWVERAKEHTIGTNAPVSLRD
                     GYRAAAKTGVCSERLWPWRPERFAERPAKRCFTEARARRALTYHRVPRELDHLRGCLA
                     QGFPFTLGISVHERFESRTVRETGRVPMPSRDERTLGGHAVLAVGYDARERRFLLRNS
                     WGRRWGMAGYFTLPFDYLLHPDLAWDFWTVRRVL"
     gene            5341..7947
                     /locus_tag="P5164_03180"
     CDS             5341..7947
                     /locus_tag="P5164_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRY42929.1"
                     /translation="MTLSGSEKVGPYEILTRIGVGGMGEVWRARDTRLGRDVAIKILT
                     RTGSSDTDRIRRFEVEMKAVGRLNHPNVLAVHDVGSHDGVPYLVSELLEGETLRDRLD
                     KGALPPKSVANTACEIAQGLAAAHAAGVIHRDLKPENVFLTRDGRVKLLDFGLAKLVE
                     PVVPLSFEGLPDAETAERLTVSGIVVGTVRYMSPEQVRGAPLDHRSDIFAFGAVLFEM
                     LTGQVAFRKPSAIETLSATLAEDPISRLDDPAKIPPELEPILRRCLEKAPEDRFQSAA
                     DLAFQLREYAEGFTRASSVRRRSFPAQRRRLAVLLPAAVAALVVLGGAALAWRAGLAA
                     GGPGVPAYKQLTFRRGLVLSARFSPDGKTIVYGAGWEGEPFRLYSMRTESPESRPLDL
                     PPGDVLAVSSAGELAVSLDRRYIYGFSARGTLARVPLVGGAPRKILTDVEDADFSPDG
                     QELAVSRVVEGRHRLEYPIEKVLHVAEGWLSNVRVSPDGERVAFVDHPIAGDDRGSIC
                     VVDRAGKVTVLAAGWASALGLAWSPKGDEVWFTATDVSPNCALWSVDLKGNRRLLARS
                     PGRLALQDVSATGELLVTEGRVRLGMGLGTAGQPADRDLSWLDATVVSDLSRDGHAVL
                     FAEQGAGGGAGTYSVYYRGTEEPAAARLGEGTAGPLSPDGKWAVAIVPTTPPSVVLLP
                     TGPGRPKTLDRGPITSYQAAAFFPGGDRLLLAANEEGKPVRLYVQSTEAGGPRAISGD
                     GFRITTSTDAVSPDGRVASVIDAEGRVVLFPLDGAEPRLAPGLEPGDVPMRWSVDGRS
                     LFVFRYDEMPARIRKIDLSTQVREVVATIAPPDPAGVESIVAAQVTPDGRSWVYSFFQ
                     YRNDLHLVTGLK"
     gene            complement(8023..8193)
                     /locus_tag="P5164_03185"
     CDS             complement(8023..8193)
                     /locus_tag="P5164_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42930.1"
                     /translation="MATKKAAKKVAKKAVKKVAKKAAKAPAKKAVKKVAKKAVKKAPA
                     KKAAKKKDVGLV"
     gene            complement(8232..10412)
                     /locus_tag="P5164_03190"
     CDS             complement(8232..10412)
                     /locus_tag="P5164_03190"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HRY42931.1"
                     /translation="MKSLLDAGASDRHIRRLIRARATTPRPEAVDVLLVSMPFGPEMA
                     PSLGLSLLKAGLAPLGVSCRVLYLTLRFAERLGVGPYTRIATDGTRSIRELAGEHVFR
                     EALFGPDPAGDARWERDVLLARACWPSKELARPVPPSFVRAVRRARSLAGPFLDRALD
                     EVLAHRPRLVGFTSVFQQHVASLALARRIKAAAPEIVVVLGGANCESVMGAETARSFP
                     FLDAVVSGEGDLVFPELVARALAGRALDDLPGVATPGNVGALFSAGRFPNAPSVTRMD
                     DLPVPDDSDYFGQFARSRFAGTWTPGLFLETARGCSWGEKSHCTFCGLNGTTMAFRSK
                     SPARALAELRDLTSRHPGSDLQVVDNILDTRYFDTLLPELAKAPPSSPVFWETKASLR
                     KDQIRALADAGVTRIQPGVESLSDAVLKLMKKGVTALQNVQLLKWCLELGVTPYWNLL
                     WGFPDEEPAEYARMARLVPLLAHLAPPSGVAGLRLDRFSPGFDDAGRLGFLDVAPMPS
                     YAAVYPFPPDRLANLAYSFTFARRGEGEAAEYAAPLLRALARWKRVAGASALFSVPAG
                     DTLFVFDLRASARRPLTVLRGEARAVHDACDASTTVRAVVERVRSECGFARDEAERAL
                     AALVRDRLLLEDGERRLALAVPLGRFAPFGRASARFHRLARRIGRRTRDGIAFPVHNA
                     AVTFRTRNGDDAPHAPQRTRTTSSSREVLLDRFSVNRRGELVFS"
     gene            10454..13681
                     /locus_tag="P5164_03195"
     CDS             10454..13681
                     /locus_tag="P5164_03195"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRY42932.1"
                     /translation="MRPRRLAVVALGALLAAFPAAPRELGRLSLDEGLSQSIVEAIHQ
                     DGLGFLWLATEDGLNRWDGARFTVFRNAPGEARSFPYNDLKCLDEDAQGALWVGSFGG
                     GLNRFDPATGRVTSYRNDPADPASLPSDLVRAVRVDRDGRIWAGTQGGGLARLDPRTG
                     RFESFRNDPRDGASLAHDDVRALLVDGRGTLWVGTYGGGLDRFDAARGAFVHVPADGG
                     PGAELVDALLEDRSGHLWAGTWGGGLFLLDEGSGRLVPRPRGARLPGERVKALAEDHE
                     GFLWIATDGAGLARYDRKTETVEVLAHDPAVPRSLATDRVFSLLEDRSRVLWIGTYGG
                     GLNTLDLGRKKFRNVRHDPRVPTSLGHDIVWSFHEDGDGTVWVGTDSGGLQRWDRPRD
                     VFRGFRHDPRNPSSLAHDTVRSLLGDAAGNLWVATNGGGLDRLDRATGRFEHHRHDPR
                     DPSSIAHDELRTLYEDRSGTLWVGTYGGGLDRLERETGRFVHHRNDPADPASLSNDFV
                     RCVLEDSAGALWVGTQGGGLNRLDRATGRFTRFRADPADPTSLSNDFVFALHEDRAGS
                     FWVGTYGGGLNRLDRATGRFTRFTARDGLSSDSVYGILEDERGRLWISTNRGLTELDP
                     AGGTFRSWDETDGLQSDEFNGGAFYRSPRGEMFFGGIRGFDVFSPSEIAVRTEAAPVV
                     LTELLLANRPVPVGRPVGGRMLLPTFVTYADEVVLSHDDDVVSVEFAALHFAAPQKLR
                     YAFRLDGFDRDWIPTGADRRVATYTGLAPGRYLFRVKAANPDGVWGEREARLKIVVTP
                     PFWGTWWFRLAAAAALAAAVVAALRRRMRTVRLAAAMRAAHDAQMAVMPHEDPEVEGF
                     EVSGVCIPALEVGGDFFDYVRSGEAGGKLGIVVGDVSGKGTPAAMAAAMSGGMVGALV
                     RRGEPLEAVAEEVNGALRAKIGKRMFAAVCLATLDPVRRELTFVNAGLCEPLLRSGDT
                     ATYLASEGSTFPLGAGARGRYRARTVPLAPGDVVILYTDGVPEAAGRAGAPWGYDALA
                     GFLRGLPAGSRTAREIRDAIVREVARVTGGPRPADDVAVVVVRAL"
     gene            13718..14677
                     /locus_tag="P5164_03200"
     CDS             13718..14677
                     /locus_tag="P5164_03200"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRY42933.1"
                     /translation="MPFSPSSPLSPGRLVTGSATFLLFALNTLVWCVLLYLVTLLKLV
                     VPFPAFRRAASRLLVAIAEAWIAVNTACLRATQPTTYDVEGLEGLRRDVSYLVVSNHQ
                     SWVDIPVLQGVFLRRIPFLRFFLKQQLIRVPFLGAAWWALDFPFMKRHSREALERNPE
                     LRHEDLAATRRACERFRHAPAAILNFVEGTRFTPAKHARGGSPFRRLLAPKAGGLAFA
                     AAAMGPQLAGIVDVTIVYPQGSPTFWDLISRGLPEVVVRARERAIPGEWFVGDYTEDA
                     AFRESVQGWVRDLFLEKDAEIDALLASRRGPAGADRAPEDGLG"
     gene            14840..>16587
                     /locus_tag="P5164_03205"
     CDS             14840..>16587
                     /locus_tag="P5164_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HRY42934.1"
                     /translation="MGVVFGDIGTSPLYALRECFTGHHPIPPTPGNVLGILSLIFWAL
                     VLTISIKYLAIVLRADNRGEGGILALMALLRPPSTAGVSRHVVLVALGLFGAALLYGD
                     SIITPAITVLSAVEGLEIVTPAFKPYILPIVILILVVLFAIQRKGTAGVGAVFGPVML
                     VWFVVLACLGLVHIFDAPRVLWAVNPLHGVRFFAENGMTGFLLLGSVFLVVTGGEALY
                     ADMGHFGAKPIRVGWFAFVLPSLLLNYFGQGALLLTNPSAAANPLFGLAPKWGLLPLV
                     LMATAASVIASQAVISGAFSLTRQAIQLGYMPRLRIDHTSEKEIGQIYLPSINWALAV
                     ACIWLVLAFRSSGALAAAYGVAVTTTMGITTILLYVYAREAWKVSRAVALPIAVAILT
                     IDLAFFGANIVKIGHGGWVPLAIALFVYTIMTTWKRGRQILSARLREASLPVDVFLQD
                     VEKRPPTRVKGTAVFMDRIPEGIPPPLLHNLKHNKILHERVVFLTVVTDEVPRLKEKE
                     RSRLEYLGPGCWRVTLHYGFMENPDVPRALRKLKLDGKGFEPMETTFFLGRETLIASK
                     RPGMALWREHFFAWMSR"
BASE COUNT         2224 a         5816 c         6159 g         2388 t
ORIGIN      
        1 agcttcgggg cctttgccgc agcctcccga acggttgcgg gcatggtgcc gatctgaagc
       61 tcgtgctgga tcgtcctccg ggacaggccc gtccgggccg cggtgtcggc ggtgaacccg
      121 agcgaaatcc tttcgccact acggacgcgc cggctcaacg tgcgcctccg gccaggagga
      181 gcgtcaggac ggcgacgagg acgcggaggg cgagcctcat gccgcacctc gggcgaatgc
      241 cgggtcggcg tgctcccggc agacctcggc gccggggtgc agcggccggc cgcaccggac
      301 gaccgcctcc ggctccggag cctcctcgtc ctcgacggtc gggcaggtgc ccggccgcca
      361 ctcggcgcgc gggaggccgt cctcgtcata ggtgaagtcg gcgtgtgtct cctgacccgc
      421 tacgggtgct acaccgctac aacccgcatg gttgtcgctc gaaaccgtag cgggtgaccc
      481 gctacatgac ccgctacacg ttccggactt cggcgtcgcc gcgggagtct gtagcgggtg
      541 ctgtagcggg tagggtgcta cgttttgacc cgcatggttg tcgctttgta gcggcgtagc
      601 gggtgtagcg gacccctcag gctgaggagg ttcggagagg tatcgctcga aggcgtcggc
      661 gaacgccgac accatgtagc cgcggacgac ggcgtcgccg tcccggatgc gagtcggccg
      721 gatgccgtag ggccggagga ggcgggcgag gtcgagagcg tcgacctcct cgcggcgcgg
      781 tccgaacctc ttccagcgag actccgggag cgccctcagc tcctcgagga gcttctccgt
      841 ccggatcgtc ccgtccgtcc tgacgaggag ggtgaggacc cggcgaacgt cggcgaggag
      901 ctggacgccg tcggactcgt cctcctcggt tcgctccccg gccagggcga cgatcgaacg
      961 ccgcgcacgc tccggccagg gtccgccggc aagatccgcg atcgcgagga gcggctcggc
     1021 cccgtcggcc gcacggtcgc ccagctcgcg cgggagcgcc ggccgggcgg agcggagctg
     1081 cgggacacgg gccgtcgccc atgcctcgag gcgggcgcgc agctcgcgcg cttccttctc
     1141 aacgtcgcgc agccggaacc gctcagccgt gtcagagcgg cgcttacgct tcatctcgat
     1201 cgggaccgat cggtcgccga ccgtgtcggg caacgtcccg atcccggcga gcgccttcgg
     1261 gcagtagacg ttgaagtcga tgagcttgtc ggacttcgcg tcgaggacgc ggggaaccgt
     1321 cacgccgcgc cggctgccgg cgttgaggag cgcccggacc gcttcggccc gagagtcgcc
     1381 gcgctgcgag aagagagcgt ccgtctcgtc gaggaggagg gtcggccgat ccttctcgat
     1441 ctttcgatag aggacggcct ccgacggctg aacggcgtgc cagggcgcgc gggtgacgac
     1501 gccgaggatc tccaggagtc gcgtcttgcc cgacctcttc tcggccgaga ggatcgcgag
     1561 gtagaccgtc tggtcggacg catcgaacgc ccaggtgtgg aggacccaga gggcgatgat
     1621 gtgggtctgc tcgggagtaa cgacgacgta gcgccgaacg aatcggacga ccgcgtcgag
     1681 gacctcagca ccgtccacgc tggcgaggct ctcagccgtc ggggcctccg ccgcgaggcg
     1741 tagcagctcg tccgccgtgc cgccggccgc gagccagtcg gatacgtctt tgacgccggg
     1801 tcggccgagg ttgagaaccc gcacctcagc ggcgacgcca acgaggtcct tcgcaatcgt
     1861 ccgcccgagg tcgcggcccg gaggatcgtc gtcggggaga aggacgacgc gggcgccgcg
     1921 gagaggctcg atatagtcag cgggcctcca cgccttggct cctcccgcgt gcgtcgtcgc
     1981 gcggagtccg acggcgcgaa ggttgtcggc gtccttctct ccctcgacgt agtagagggg
     2041 gaggccggag cggaccgcct cgaggacctc cgggagacgg tagagaaccc ggcgcgtccc
     2101 gttcagattc caggcgaagc cgtcctttga cttcgggtcg ggccgacgct ggcggaagtc
     2161 cttcggctcg aagcggacga cctcatagag gagggcgccc gtctcgtccg ggtagcggta
     2221 aacggccttg atgcgcttcg atccgcgacg ctcctcccgc tccggcgaga tgtccgcgac
     2281 cgccaggccg agcgcgccga ggattgcctc gaggtcgcat ccggcgtgac acttcacgag
     2341 ggcgcggccg tccctgccct gcgagactga gagggaggcg gtccggtcct catgcgccgg
     2401 gcattgccag gcggagccct tgccgcgcga gccgtgcgcc tcgagggccg agacgacggt
     2461 tttgaacggg gtcatgccgc cccccgagtc gcccagcggc cgagccggcc gacggctaga
     2521 tcccggtcga cgaggacggg gctggagtta ggatctgacg acgcgctaga atgcggcttg
     2581 caggcggttt tcgatcgcgt cggccggttc gggtgagcta gacccgagcc ggctttttcg
     2641 ttttcgtcgc ctctcatccg gggctcccgg cgaaccggcg aagctcgccg aaggggatca
     2701 gaatcgcccg cccccgccga atcgcgcgga tctcaccgcg cgcgatcgca gcggcgatgc
     2761 cgcggacgga caggccgacg agtgccggtg catcggcgag gcgaacgcaa agcggctcgg
     2821 ccgcgggctt cggacgacgg cgggagggag ggaggttaga ggtcagggct ttcggcatga
     2881 ggtcctttct cggctgcgcg cagccgcgtt acggggtgac gggcagacac ggcaaagccg
     2941 cacggcgagc gccgcgcgga cggagagcga ctagctgcgg gaaggggggc gtcagcggcg
     3001 gaacgtggac cgggagcctg aggctcttta ccggggctcg atcggtgccg ctgctgctgt
     3061 tagtgatctt gcctttcagc aagatcggcg gaggtcggac cccatagggt gcctcccact
     3121 gattgggcga gactttagcg cacggtgagg aactctgcaa gaggagcccc gcgcctgcgg
     3181 catccgcaat acgacttggg tctaggtgca acctggagtt gtacggggtt gcctgcacgt
     3241 cccggcctcc cgcacgtccc aggcgaaagg gtcgggggcg aggacgcggg ggacgtgctg
     3301 tccgctcggc cgggggatca atcccggccg cctcgccccc gacgggctac ttcatgcaga
     3361 ggcgttcacg acggtcactc tcgacgggag cggggccgcg tcacttttcg cgtcaccgcg
     3421 tcacccccgg agtgtgctcc gtgcgctcgc ccggcgcaag agaaagcggg ctaagtgcag
     3481 taagaatgcg ggtttctgtc gtgtgactgg tagggggcga aagcgccgaa aaggaggtgt
     3541 cggaggactt ctaagccgag ggtcgccggt tcgagcccgg ccgggcaggc cagccgttcc
     3601 gtccggcacc ggctcgtcga tcctcccgcg tctgcttcac cggcgcgtcc gcccgatcgc
     3661 gagcaggttg ttcccgcgcg cggccgcttc ttccgagagg cagagagcgg cctcgagcgg
     3721 gacgagccgg cgccggagcc gggggctcgc gaggtgcgcg gggagcgcct cgtcggggcc
     3781 ctcggggtgg acggtgaccg gaaagccgcg caccgagacc tccgtgcagc cggcccgctg
     3841 gaggagcgcg cgggcgatcg ccggggtgaa gacgaggacc tcggggactc cgggcgcata
     3901 gcggacggag gcgcttgcgg cgagccggcg gagctcggac ggacggccgt cgcggaggac
     3961 cttcgccgcc gcctgccaga ggctgggaag gacgaacgcg acgcggcccc tgcccacggc
     4021 cgcacgcgcg aggcgagcga agagcgcggc gggatcggcg acgaggctgg cgacgttgtg
     4081 gaagcagagg acgaggtcga agcggccgag gcggcgcggc agcggctcgt ggaggtcgtg
     4141 cgcgaggagc gagagccggc ccaagagccc ggcccggacg gccttgcgcc gggcggcggc
     4201 gagcatcccg ggggagacgt cggcgaggac ggcggtcgca cgcgggagct cgcggagcag
     4261 gtggagcgcc cagcgcccgg ttccgccgcc ggcgtcgaga aggcggagac cgtctccgtc
     4321 gccgaggccg gtcaggatcg tccgccggag gagctcgagg aggacccggt cggaaaagtc
     4381 ccagtagggg cggtccgcca cacggtcgta gacgcgcgcg cgtcggtcga agaaggcgcg
     4441 gacgtcggcg ctgcgcttct cggggtcgag cttcggccag ggagcggcgg gggactcggc
     4501 gctggacatg ggagccgcga gaatagcgcc cgcctggcaa ggagaagcga atgacgaagg
     4561 cccggccgta cggcacgcac cccgacgtcc ccgaccatcg cgacctgcgc tgggaggcgc
     4621 cgcggcacgt gcggcgcgcg ctccccgccg cggtcgacct gcggcccggc tgtccgcccg
     4681 tcgaggacca gcgacctctc aacgcctgct ccgctcacgc gatcggcgcg gcgctgtggt
     4741 acgacgagcg gcgccaggga gaggaggctc cgctgccgtc gcggctcttc ctctactggg
     4801 tcgagcgggc gaaggagcac acgatcggga cgaacgcgcc ggtgtcgctc cgcgacggct
     4861 accgggcggc ggcgaagacc ggcgtctgct cggagcgcct ctggccctgg cggcccgaga
     4921 ggttcgccga gaggccggcg aagcgatgct tcacggaggc ccgggcgcgc cgggcgctga
     4981 cgtatcaccg cgtcccgcgc gagctcgacc acctccgggg ctgcctcgca cagggcttcc
     5041 cgttcacgct cgggatctcg gtgcacgagc gcttcgagag ccggacggtg agggagacgg
     5101 ggcgggtgcc gatgccctcg cgggacgaga ggacgctcgg cgggcatgcg gtcctcgcgg
     5161 tcggctacga cgcgcgggag cgccgcttcc tcctccggaa ctcctggggg aggcgctggg
     5221 ggatggcggg atacttcacg ctccccttcg actacctcct tcaccccgac ctcgcctggg
     5281 acttctggac cgttcgacgc gttctctgag cgggccggac gcgctaccat ccgtcggcgc
     5341 atgacgctct ccggcagcga gaaagtcggt ccctacgaga tcctgacccg catcggcgtg
     5401 ggagggatgg gcgaggtctg gcgggcgcgg gacacgcggc tcgggcgcga cgtcgcgatc
     5461 aagatcctga cgaggaccgg ctcctccgac accgacagga tccgccggtt cgaggtcgag
     5521 atgaaggcgg tcgggaggct gaatcacccg aacgtcctcg ccgtccacga cgtcgggagc
     5581 cacgacggcg tgccgtacct ggtcagcgag ctgctcgagg gggagacgct ccgcgatcgg
     5641 ctggacaagg gtgcgctgcc gccgaagtcc gtcgcgaaca ccgcctgcga gatcgcgcag
     5701 gggctcgccg cggcgcatgc cgccggggtc atccaccgcg acctgaagcc ggagaacgtc
     5761 ttcctcacgc gcgacggccg tgtgaagctc ctcgacttcg gcctcgcgaa gctcgtcgag
     5821 ccggtcgtgc cgctttcgtt cgaggggctc cccgacgccg agaccgccga gcggctgacc
     5881 gtctccggga tcgtcgtcgg cacggtgagg tacatgtcgc ccgagcaggt ccgcggcgcg
     5941 ccgctcgacc atcgctccga catcttcgcg ttcggcgcgg tcctcttcga gatgctgacc
     6001 gggcaggtcg cgttccggaa gccgtcggcg atcgagacgc tgagcgcgac gctcgccgag
     6061 gacccgatct cgcgcctcga cgatcccgcg aagatccctc cggagctcga gccgatcctc
     6121 cgccgctgcc tcgagaaggc gccggaggac cggttccagt ctgcggccga cctcgccttc
     6181 cagctccggg agtacgcgga gggcttcacc cgggcctctt cggtccggcg gcggtcgttc
     6241 cccgcgcagc gtcggcgcct cgcggtcctc ctcccggccg cggtcgccgc gctcgtcgtg
     6301 ctcggaggcg cggccctcgc ctggcgcgcg gggctcgcgg cgggagggcc gggcgtgccg
     6361 gcctacaagc agctgacgtt ccggaggggc ctcgtcctct cggcgcgctt ctcgccggac
     6421 ggaaagacga tcgtctacgg cgcgggctgg gaaggggagc cgttccgcct ctactcgatg
     6481 cggacggaga gcccggagtc ccgcccgctc gaccttcccc cgggagacgt cctcgcggtc
     6541 tcgtcggccg gcgagctcgc cgtctcgctg gatcgccgat acatctacgg cttctcggcg
     6601 agagggacgc tcgcgcgcgt cccgctcgtc ggcggggcgc cgcggaagat cctgaccgac
     6661 gtggaggacg cggacttctc gcccgacggc caggagctcg cggtgagccg cgtcgtcgaa
     6721 gggcggcacc gcctcgagta cccgatcgag aaggtcctcc atgtcgccga ggggtggctc
     6781 agcaacgtgc gggtctcccc cgacggcgag cgggtcgcgt tcgtcgacca cccgatcgcc
     6841 ggcgacgacc gcggctcgat ctgcgtcgtc gaccgcgccg gcaaggtgac cgtcctcgcc
     6901 gcgggctggg cgagcgccct cggcctcgcg tggtcgccga agggggacga ggtctggttc
     6961 accgcgacgg acgtctcccc gaactgcgcg ctctggtccg tcgacctgaa ggggaatcgg
     7021 cgcctcctgg cgcggagccc ggggcgcctc gcgctccagg acgtgagcgc gaccggcgag
     7081 ctcctcgtga cggaagggcg cgtccggctc gggatggggc tcggcaccgc ggggcagccg
     7141 gccgacaggg acctctcgtg gctcgacgcg acggtcgtct cggatctctc gcgcgacggg
     7201 cacgccgtcc tcttcgccga gcagggagcc gggggaggcg cggggacgta ctcggtctac
     7261 taccgcggga cggaggagcc cgccgcggcg cggctgggcg aggggacggc cggcccgctc
     7321 tcgcccgacg ggaagtgggc ggtggcgatc gtcccgacca cgcctccgag cgtcgtcctc
     7381 ctcccgacgg ggccgggccg tccgaagacg ctcgatcgcg gtccgatcac gagctaccag
     7441 gcggcggcct tcttccccgg cggtgacagg ctgctgctgg cggcgaacga ggaagggaag
     7501 ccggtccggc tctacgtgca gagcacggaa gccggcgggc cgcgcgcgat ctccggggac
     7561 ggcttccgga tcacgacgag cacggacgcg gtgtcccccg acgggcgcgt cgcgtcggtg
     7621 atcgacgccg aggggcgcgt cgtcctcttc cctctcgacg gggcggagcc gcgcctggcg
     7681 cccggcctcg agccgggtga cgtcccgatg cgctggagcg tcgacggccg ctcgctcttc
     7741 gtcttccgct acgacgagat gcccgcccgc atccggaaga tcgacctctc gacgcaggtg
     7801 cgggaggtgg tcgcgacgat cgccccgccg gacccggcgg gcgtcgagtc gatcgtcgcg
     7861 gcccaggtga cgcccgacgg gcgctcctgg gtctacagct tcttccagta caggaacgac
     7921 ctccacctcg tcaccggcct gaagtagggg gccgaggcgg cgccgggaga tcgcggcgcg
     7981 gagccggccc cgcgagggga ccggctccgg cgcgacggtc gatcagacga gaccgacgtc
     8041 cttcttcttg gcggccttct tggcgggggc cttcttgacg gccttcttgg cgaccttctt
     8101 gacggccttc ttcgccgggg ccttggcggc cttcttggcg accttcttca cggccttctt
     8161 cgcaaccttc ttcgctgcct tcttcgttgc catccggatc tcctttcggt tccgccctcg
     8221 aggacggggg ttcaggaaaa gaccagctct ccgcgacgat tcacggagaa gcggtcgagt
     8281 agaacttcgc gggaggaaga cgtcgtccgc gttctctgcg gggcgtgcgg cgcgtcgtcc
     8341 ccgtttcgtg tccgaaacgt caccgctgca ttgtgaacgg gaaacgcgat tccgtcacgc
     8401 gtccttcgcc cgattcttcg cgcgaggcga tggaagcgcg cgctcgctcg cccgaaggga
     8461 gcgaagcgtc cgagcgggac ggcgagcgcg aggcggcgct cgccgtcctc gaggaggagg
     8521 cggtcgcgca cgagcgcggc gagcgcgcgc tccgcttcgt cgcgcgcgaa gccgcattcc
     8581 gagcggactc tctcgacgac cgcgcggacg gtcgtcgagg cgtcgcacgc gtcgtggacg
     8641 gcgcgcgcct ctccgcgaag gaccgtcagc gggcgccgtg cggaggcccg gaggtcgaag
     8701 acgaagagcg tgtcgcccgc cggaacggag aagagggcgc tcgcgccggc gacgcgcttc
     8761 caccgcgcga gcgcgcggag gagaggcgcc gcgtactcgg ccgcctctcc ttcgccgcgg
     8821 cgcgcgaacg tgaaggagta ggcgaggttc gcgaggcggt cgggcgggaa cgggtagacc
     8881 gcggcgtacg acggcatcgg cgcgacgtcg aggaagccga ggcggccggc gtcgtcgaag
     8941 ccggggctga agcggtcgag acgcaggccg gcgacgccgg acggcggcgc gaggtgggcg
     9001 aggagcggca cgagccgcgc catccgcgcg tactcggcgg gctcctcgtc ggggaagccc
     9061 cagaggaggt tccagtaggg cgtgacgccg agctcgaggc accacttcag gagctggacg
     9121 ttctggagcg cggtgacgcc cttcttcatg agcttcagca cggcgtcgga gaggctctcg
     9181 acgcccggct ggatgcgtgt gacgcccgcg tcggcaagcg cgcggatctg gtccttccgg
     9241 aggctcgcct tcgtctccca gaagacgggc gaggacgggg gcgccttcgc gagctcgggg
     9301 aggagcgtgt cgaagtagcg cgtgtcgagg atgttgtcga cgacctgcag gtcgcttccc
     9361 gggtgccgcg acgtgaggtc gcgcagctcg gcgagggcgc gcgcggggga cttgctccgg
     9421 aacgccatcg tcgtgccgtt gaggccgcag aacgtgcagt ggctcttctc tccccaggag
     9481 cagccgcggg ccgtctcgag gaagaggccg ggcgtccagg tcccggcgaa gcggctccgc
     9541 gcgaactggc cgaagtagtc ggagtcgtcg gggacgggga ggtcgtccat ccgggtgacc
     9601 gacggggcgt tcgggaagcg ccccgccgag aagagcgccc cgacgttccc cggcgtcgcg
     9661 acgccgggga ggtcgtcgag cgcacggccc gcgagggcgc gcgcgacgag ctccgggaag
     9721 acgaggtccc cttcgcccga gacgacggcg tcgaggaacg ggaagctgcg cgcggtctcg
     9781 gcccccatga cgctctcgca gttcgcgccg ccgaggacga cgacgatctc cggcgcggcg
     9841 gccttgatcc ggcgcgcgag ggcgagcgag gcgacgtgct gctggaagac gctcgtgaag
     9901 ccgacgaggc gcggccggtg cgcgaggacc tcgtcgagcg cccgatcgag gaacggtccg
     9961 gcgaggctcc gcgcgcgccg gaccgctcgg acgaacgagg gagggacggg acgggcgagc
    10021 tccttcgagg gccagcatgc ccgggcgagg aggacgtcgc gctcccagcg cgcgtcgccc
    10081 gccgggtcgg gaccgaacag agcctcgcgg aagacgtgct cgcccgcgag ctctcggatc
    10141 gaccgggtgc cgtcggtcgc gatccgcgtg taggggccga cgccgaggcg ctcggcgaag
    10201 cggagcgtca ggtagaggac gcggcaggag acgccgagcg gcgcgaggcc ggccttcagg
    10261 agggagaggc cgagcgacgg cgccatctcg gggccgaacg gcatcgagac gaggaggacg
    10321 tcgacggcct ccgggcgggg tgtggtcgcg cgggctcgga tgaggcggcg aatgtgccgg
    10381 tccgaggcgc ccgcgtcaag gagcgacttc atcgcaggac gaacccgcgc cgcgccgggc
    10441 cgtaccggag agcatgaggc cccgtcgcct cgccgtcgtg gcgctcggcg ccctcctcgc
    10501 ggcattcccc gccgcgccgc gggagctggg gcgcctctcg ctcgacgaag ggctctccca
    10561 gagcatcgtc gaggcgatcc accaggacgg gctcggcttc ctctggctcg cgacggaaga
    10621 cggcctgaac cgctgggacg gcgcccgctt cacggtcttc cggaacgcgc cgggcgaggc
    10681 gcggagcttc ccgtacaacg acctgaagtg cctcgacgag gacgcgcagg gggcgctctg
    10741 ggtcggctcg ttcggcggcg gcctgaaccg gttcgacccg gcgacgggcc gcgtcacgtc
    10801 gtaccgcaac gacccggccg atccggcgag cctcccttcc gacctcgtcc gcgccgttcg
    10861 cgtcgaccgc gacgggcgga tctgggccgg aacgcagggc ggcggcctcg cgcgcctcga
    10921 cccgcggacg gggcgcttcg agagcttccg gaacgacccg cgcgacgggg cgagcctcgc
    10981 gcacgacgac gtccgggcgc tcctcgtcga cggacgcggg acgctctggg tcggcacgta
    11041 cgggggaggg ctcgaccggt tcgacgcggc gcgcggcgcc ttcgtccacg tccccgcgga
    11101 cggagggccc ggcgcggagc tcgtcgacgc cctcctcgag gaccgctcgg ggcacctctg
    11161 ggccgggacg tggggcggag ggctcttcct cctcgacgag ggctcggggc gcctcgtccc
    11221 tcgcccgcgc ggcgcgcgcc tgccgggaga gagggtgaaa gcgctcgcgg aggaccacga
    11281 gggcttcctc tggatcgcga cggacggggc gggcctcgcc cggtacgacc ggaagacgga
    11341 gacggtcgag gtcctcgcgc acgaccccgc cgtcccgcgg agcctcgcga ccgaccgcgt
    11401 cttctcgctc ctcgaggacc gttcgcgggt cctctggatc gggacgtacg gcggcgggct
    11461 gaacacgctc gacctcggcc ggaagaagtt ccggaacgtc cggcacgacc cgcgcgtccc
    11521 gacgtcgctc gggcacgaca tcgtctggtc cttccacgag gacggcgacg gaacggtctg
    11581 ggtcgggacc gactccggcg ggctgcagcg ctgggaccgc ccgcgcgacg tcttccgcgg
    11641 cttccggcac gacccgcgga atccctcctc cctcgcgcac gacacggtcc gttcgctcct
    11701 cggcgacgcg gccgggaacc tctgggtcgc gacgaacggc ggcggcctcg accgtctcga
    11761 ccgggcgacc ggccgtttcg agcaccaccg gcacgatccg cgcgacccct cctcgatcgc
    11821 gcacgacgag ctccggacgc tctacgagga ccggtcgggg acgctctggg tcgggacgta
    11881 tggcggcggg ctcgaccggc tcgagcgcga gacggggcgc ttcgtccacc accggaacga
    11941 cccggccgat ccggcgagcc tctcgaacga cttcgtccgg tgcgtcctcg aggactcggc
    12001 cggcgcgctc tgggtcggga cgcagggggg cgggctgaac cgcctcgacc gcgcgacggg
    12061 ccgcttcacg cgcttccggg cggacccggc cgaccccacg agcctctcga acgacttcgt
    12121 cttcgctctc cacgaggacc gcgccgggag cttctgggtc gggacctacg ggggcgggct
    12181 gaaccgcctc gaccgcgcga cgggccgctt cacgcggttc acggcgcggg acggcctctc
    12241 gagcgactcg gtgtacggga tcctcgagga cgagcggggc cgcctctgga tctcgacgaa
    12301 ccgcgggctg acggagctcg acccggccgg cgggacgttc cgctcctggg acgagacgga
    12361 cggccttcag agcgacgagt tcaacggcgg cgccttctac cgcagccccc gcggggagat
    12421 gttcttcggc ggcatccggg gcttcgacgt cttctccccg tccgagatcg cggtccggac
    12481 ggaggcggcc cccgtcgtcc tgacggagct cctcctcgcg aaccggcccg tcccggtggg
    12541 ccgtcccgtg ggcgggagga tgctcctgcc gacgttcgtc acctacgcgg acgaggtcgt
    12601 cctctctcac gacgacgacg tcgtctcggt cgagttcgcg gcccttcact tcgcggcgcc
    12661 gcagaagctc cggtacgcgt tccgcctcga cggcttcgac cgcgactgga tcccgaccgg
    12721 ggccgaccgg cgcgtcgcga cgtacacggg gctcgcgccg gggcggtacc tcttccgggt
    12781 gaaggccgcg aaccccgacg gcgtctgggg ggaacgcgag gcgcgcctga agatcgtcgt
    12841 gacgccgccg ttctggggta cctggtggtt ccgcctcgcg gcggcggccg cgctcgccgc
    12901 cgccgtcgtc gcggcgctcc ggcggaggat gcggacggtg cgcctcgcgg cggcgatgcg
    12961 ggcggcccac gacgcgcaga tggccgtcat gccgcacgag gacccggagg tcgaggggtt
    13021 cgaggtttcg ggggtctgca tcccggccct cgaggtgggc ggggacttct tcgactacgt
    13081 gaggagcggc gaggcgggcg ggaagctcgg gatcgtcgtc ggcgacgtct cggggaaggg
    13141 gactccggcc gcgatggcgg cggcgatgtc gggcgggatg gtcggcgccc tcgtgcggcg
    13201 cggcgagccg ctcgaggccg tggccgagga ggtcaacggc gccctccgcg cgaagatcgg
    13261 gaagaggatg ttcgccgcgg tgtgcctcgc gacgctcgac ccggtgcgcc gcgagctgac
    13321 cttcgtcaac gcggggctct gcgagccgct cctccgctcg ggcgacacgg cgacctacct
    13381 cgcctcggaa ggaagcacgt tcccgctcgg cgccggcgcg cggggccgct accgcgcgcg
    13441 gacggtgccg ctcgctccgg gcgacgtcgt gatcctctac accgacggcg tccccgaggc
    13501 ggcgggccgc gcgggggcgc cgtggggcta cgacgcgctc gccgggttcc tgcgcggcct
    13561 gcccgcgggc tcgcggaccg cgcgggagat ccgcgacgcg atcgtccggg aggtcgcgcg
    13621 cgtcaccgga gggccgcgcc ccgcggacga cgtcgccgtg gtcgtcgtcc gggcgctctg
    13681 agcctcgggc cggtccgcgg ccgtaccatt cggcgcgatg ccgttctccc cttcctcgcc
    13741 cctttccccg ggtcggctcg tgaccgggag cgcgacgttc ctcctcttcg cgctgaacac
    13801 gctcgtctgg tgcgtcctcc tctacctggt gacgctcctg aagctcgtcg tcccgtttcc
    13861 cgcgttccgc agggccgcct cgcgcctcct ggtcgcgatc gccgaggcct ggatcgcggt
    13921 gaacaccgcc tgcctgcgcg ccacccagcc gaccacctac gacgtggagg ggctcgaggg
    13981 tctgcggcgg gacgtctcct acctcgtcgt gtcgaaccac cagtcgtggg tcgacatccc
    14041 ggtcctccag ggcgtcttcc tccgccgcat ccccttcctc cggttcttcc tgaagcagca
    14101 gctgatccgc gtgccgttcc tcggcgccgc gtggtgggcg ctcgacttcc cgttcatgaa
    14161 gcgccactcg cgggaagcgc tcgagcggaa ccccgagctc cggcacgagg acctcgcggc
    14221 cacgcggcgc gcctgcgaga ggtttcggca cgcgcccgcc gcgatcctga acttcgtcga
    14281 ggggacgcgg ttcacgcccg ccaagcacgc gcgcggcggc tcgccgttcc gccgcctcct
    14341 cgccccgaag gcgggcggcc tcgcgttcgc ggccgcggcg atggggccgc agctcgccgg
    14401 catcgtcgac gtgacgatcg tctaccccca gggctcgccg accttctggg acctgatctc
    14461 gcgaggcctc ccggaggtcg tcgtgcgggc gcgggagcgg gcgatcccgg gagaatggtt
    14521 cgtgggggac tacaccgagg acgccgcgtt ccgcgagtcg gtccagggct gggtccgtga
    14581 cctcttcctc gagaaggacg ccgagatcga cgccctcctc gcctcccgga ggggccccgc
    14641 cggggccgat cgggcgccgg aagacggcct gggctagact tccgccccgt tgaagtcgga
    14701 ctccggcgag tcttcggcct cgcacccccc atcgcggtcg ccctccgggg ctttcgaagc
    14761 cacctcggcc ggcacggaga agacccacct cccgccggcg ccggagggga agtacctcgc
    14821 ggtcctgacg ctcggcgcca tgggcgtggt cttcggcgat atcgggacga gccccctcta
    14881 cgccctccgc gagtgcttca ccgggcacca cccgatcccc ccgaccccgg ggaacgtcct
    14941 cgggatcctg tccctcatct tctgggcgct cgtcctgacg atctcgatca agtacctcgc
    15001 gatcgtcctg cgcgccgaca accgggggga gggaggcatc ctcgccctca tggcgctcct
    15061 gcggcccccg tcgacggccg gcgtgtcgag gcacgtcgtt ctcgtggcgc tcggtctgtt
    15121 cggcgccgcg ctcctctacg gggacagcat catcacgccg gccatcaccg tcctgtccgc
    15181 ggtcgagggg ctcgagatcg tcacgcccgc tttcaagccc tacatcctgc ccatcgtcat
    15241 cctcatcctc gtcgtcctct tcgcaataca gaggaaggga acggcgggag tcggggcggt
    15301 cttcgggccg gtgatgctcg tctggttcgt cgtgctcgcc tgtctcgggc tcgtccacat
    15361 cttcgacgcg ccgcgggtcc tctgggcggt caacccgctc cacggggtcc gcttcttcgc
    15421 ggagaacgga atgacgggct tcctcctcct cggctcggtg ttcctcgtcg tcacgggggg
    15481 cgaggcgctc tacgccgaca tggggcactt cggggcgaag ccgatccgcg tcgggtggtt
    15541 cgcattcgtg ctgccgtccc tcctcctgaa ctacttcggc cagggggcgc tcctcctgac
    15601 gaacccgtcg gcggccgcga acccgctctt cgggctcgct ccgaagtggg ggctcctccc
    15661 gctcgtcctc atggcgacgg cggcctcggt catcgcgtcg caggccgtca tctcgggcgc
    15721 gttctccctc acgcgtcagg cgatccagct cggctacatg cctcgcctga ggatcgacca
    15781 cacgtcggag aaggagatcg gccagatcta ccttccttcg atcaactggg cgctcgccgt
    15841 cgcctgcatc tggctcgtcc tggcgttccg gtcgtcgggg gcgctcgccg cggcctacgg
    15901 cgtcgccgtc acgacgacga tggggatcac gacgatcctc ctctacgtct acgcgcgcga
    15961 ggcctggaag gtctcccgcg cggtcgcgct accgatcgcc gtcgcgatcc tcacgatcga
    16021 cctcgccttc ttcggggcga acatcgtcaa gatcggtcac ggcggatggg tgccgctcgc
    16081 catcgcgctc ttcgtctaca cgatcatgac gacctggaag cgcgggcggc agatcctctc
    16141 cgcccgcctc cgcgaggcct cgctcccggt cgacgtcttc ctccaggacg tcgaaaagcg
    16201 ccctccgacg cgcgtgaagg ggacggcggt cttcatggac cggatcccgg aggggatccc
    16261 gccgccgctc ctccacaacc tgaagcacaa caagatcctc cacgagcggg tcgtcttcct
    16321 gacggtcgtc accgacgagg tgccgcggct gaaggagaag gagcgctcgc gcctcgagta
    16381 cctcggcccc ggctgctggc gggtgacgct ccactacggc ttcatggaga accccgacgt
    16441 gccgcgcgcg ctccggaagc tgaagctcga cggcaagggg ttcgagccga tggagacgac
    16501 gttcttcctc ggccgcgaga ctctgatcgc gagcaagcgc cccgggatgg cgctctggcg
    16561 ggagcacttc ttcgcctgga tgtcccg
//
LOCUS       DAOTEZ010000037        16501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2381_length_16501_cov_6.629089, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000037 DAOTEZ010000000
VERSION     DAOTEZ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 16501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16501
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2381_length_16501_cov_6.629089"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="P5164_03210"
     CDS             <1..237
                     /locus_tag="P5164_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42935.1"
                     /translation="EVLRVESGTFSDTHAIAVFLAMKDNEWLTPARFEPGTLVKVRLV
                     PFKAAPSAVRSIRRVDDLDDFERIPYYVLDWEIR"
     gene            234..1442
                     /locus_tag="P5164_03215"
     CDS             234..1442
                     /locus_tag="P5164_03215"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42936.1"
                     /translation="MKSWPRLAPGLVLGLLLAAPTAADAATPPAAPRKARSARPPAAT
                     APSRAESIAAARRLLLGQLAASGAGSWDAWSASVEPFRASLRERVAAAKPNNPDSKGY
                     TESRYEVLESLGEPPRFEAWPSSYVRYVANAPVSTSEFLTRTAFVSSIEAISRWLASR
                     GIDFIFVPVPKMTEVYPEAFVAAAPADRIVAPQMRRAIFELLERNVEVVDLLPLFLEA
                     RTRPGAPSLYQHADPHWAWEAQKIALEEIVRRLRRYPEVRKSLAAPRRFRTWTERRDL
                     TSVSAAFGALTPEQARRVKPFLTANVEFAAPLGETPLASEDSPIALAGDSYNNGLEGG
                     LAARLNMPIRDLHASGGSLAPFKDLLRDPESMKSMKVLIFLVNNQALWEFPATLPGEI
                     AKFQVSRPPD"
     gene            complement(1473..2231)
                     /locus_tag="P5164_03220"
     CDS             complement(1473..2231)
                     /locus_tag="P5164_03220"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY42937.1"
                     /translation="MRGLAVAVRIELLKLKRTLALATCFVAPLVVVALQVAIWLRMKE
                     GFGADVEPWLAFHKNALSMWGVFMLPLATALVVALVSHAEHANQGWLRLGVLPLPRWV
                     VPAAKALVATGLLLLATGVLVLGCLGGAVLADALHPKIALETAAPVATMLGRTARVFG
                     AALLVLAIQNAVSLRFPSIVVSLGTGVAGTFLALFASGSRLGPYYPWLMPLHALHGKE
                     EAVVLSVALGAAGGLVLLALTVALAARRDPGLKG"
     gene            complement(2228..3151)
                     /locus_tag="P5164_03225"
     CDS             complement(2228..3151)
                     /locus_tag="P5164_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009402793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY42938.1"
                     /translation="MESVVTTCGLTYRYGETAVVSGVDLAVPRGSVYGFVGPNGAGKT
                     TTIKLLLGLLTPSGGSVELLGEPVNGRRSAALARVGALVESPALYPHLTAEENLRLDQ
                     LARGVPRANVERALAAVGLAGEGKKLVRRFSLGMKQRLGLALALLHAPSLLVLDEPSN
                     GLDPAGVRELRETIRRLAAEEGITVLVSSHILAELESVATWIGVLARGRLRFQGPLEE
                     LKGTQPGVLGVRVDDPARAQALLRRVDLSATLRPDGLLAVGVAGREGIARVNTLLVGE
                     GLAVSHLALEEASLERAFLALVAGVPEGAAA"
     gene            complement(3152..4465)
                     /locus_tag="P5164_03230"
     CDS             complement(3152..4465)
                     /locus_tag="P5164_03230"
                     /inference="COORDINATES: protein motif:HMM:NF016994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HRY42939.1"
                     /translation="MPDTTGRGAPRRARRAFVCAGLLALAAAAGCRGRDLAPTPESRW
                     IEARAVPLRGIDPAEASLDDLEPLRAAIGDARLVLLGEQTHGEGSTFLAKTRLVRFLH
                     ERMGFDVLAMESGLFACERAGDAILRGAPAREAVRGSVFEVWAESEEFRPMVEHLGAS
                     ARTRAPLELTGFDMQLTGRISREELSPALRAAAGLAPGELEAFFGPLGKLLESPAKGF
                     RKAPAKEREAFAAAAAELERRLAARADAEGAFLAQVVRSTAVDARFLWDVDFEKPAPE
                     VMNRRDAQMAENLLWLMSERYRGRKVVAWAATSHASRRRDLVERPFPDPEMVPMGQRL
                     RDELGEGAYVVAFTSGAGRVGSVFSSPWDLAPPRRGSFEDHAERTGLPALFLDLRRNA
                     PGSWLELPVKARPMGQATMTAPWPRLFDAFVFLRTAAPSRRVEEE"
     gene            4600..5271
                     /locus_tag="P5164_03235"
     CDS             4600..5271
                     /locus_tag="P5164_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42940.1"
                     /translation="MQDLVGGFLAAPLAVQAVLVCFALAFLAMVFARPLAHRRARERF
                     AALAASMGAPVAPGRSPLEASFGAVCEGRRFEVRRELRSGIAGSHYRGPRGNLVVLET
                     PLSGSRWEMHGVEIVPGGPRKRPGARALETGDPDFDARFAVWQDGVPVREGWLDAPTR
                     AAVTAFFDGPGVAGPVWAQEGRLTYVAVPPARLDEPKLTAILRRQAALASALERTAGW
                     RGPAA"
     gene            complement(5315..7084)
                     /locus_tag="P5164_03240"
     CDS             complement(5315..7084)
                     /locus_tag="P5164_03240"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRY42941.1"
                     /translation="MRWSNYFLSTLREAPGDAEVVSHKLLARAGMLLKAAAGIYSFTP
                     LGFRSLKKMTEIVREEMNRTGALEVELPIAQPKELWVESGRWDRYMNDGILFHLEDRK
                     ATEFALAPTAEEAVTDMVRRSVTSWRQLPFNLYQIRTKFRDEIRPRFGLMRGREFLMK
                     DAYSFDVDPKGLDAHYRKMDAAYRAIFERCGLEYALVQADSGAIGGSASEEFMVVADT
                     GEDALVFSEDGKYGANVEKAVTAPLPEPWANEEEKPFGRADSPTPGIVSTEGQAKHLG
                     TTEDRITNTMLYEAIRADDSSFVCAIVIRGDLTINEVKLPKALGVAHVRLVPEEWLPE
                     IAGCKPGFIGPIGLKPRKGQDEVPVYVDRSMENARNVFCGANEDGTHHSNLSVPRDLK
                     VAGVIEVATARDGDPSPTGAGVLRIKRGIEVGHIFKLGTKYSEAMKCEVADPQQKMVP
                     LQMGCYGLGVGRTVAAAVEQNHDDDGIIWPVPLAPFQVALLSLQRDKEVLEAAEKIYA
                     ELTEAGVEVFWDDRDERPGVKFKDADLIGFPVRVVVGGKALAKGVVEVSTRREKKAEG
                     VPPAKLLEVVKATLAAGAKTLAL"
     gene            7180..8445
                     /locus_tag="P5164_03245"
     CDS             7180..8445
                     /locus_tag="P5164_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42942.1"
                     /translation="MDARPFDFVPVASPLHDAAALRRALEPYLGALVGLGGRETGPEA
                     ASGAGPLAIVVGTGGTEAAVLRLREARQAAAPGEPLLLVTLPAHNSLPAALEALARVR
                     QDGGRGRIVPLHGPDDGPGLVRLAAALEDLRVRKQLHRARIGLVGTPSDWLVASVPAA
                     AAVRETWGPEVVPADLAPLLEAPLRPPDEAAAGLAASYTSGPASVRETDEAGVKTAAS
                     VLPALADLAARERLDAVAVRCFDLVTERGTSGCLALSALNDSGTVAACEGDLATAVAM
                     LWVKELLGTASWMANPSDADAVTGRLRLAHCTVPRALTSGWDLRSHFESGLGVALAGE
                     LPPGPVTLLRLGGRRLESVFLAEGEALPTPRREDLCRTQLDVLLEPDAVAELLERPLG
                     NHLVLVPGHVAARLRAYREWAVGGESREG"
     gene            8551..10755
                     /locus_tag="P5164_03250"
     CDS             8551..10755
                     /locus_tag="P5164_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRY42943.1"
                     /translation="MRLLRAALLAPLALLALLAAPRLEAARYRVVDENGRAVPGARVS
                     ILGRAGSVAAGPEGELTLEAEPALPFEVGVFSPTGAWLGIVRVETRASGDGVRDLVLP
                     AARSTEVLVSAGAAASLLAPPANPVSLVSKRELEERRPNRLVEAIETLPGISKSDESA
                     SAVPVIRGLARGRTLVMVDDGRVSAERRAGASASYLNPFALESVEVVRGPGSVAYGSD
                     ALGGIVHARTPLPEPGVFRARYQLGGGVGGSPEASAGVGLNVPLGPAAFLVHLYQRSA
                     GDYDSPAGEQDNSAVRDRGFLVRGAIPLGKARLFAGFQLDAVRDMGKPQADLPTTRTF
                     YPWEDSRRFSLGLDAPDVAGFTSVELRAFYGSYGISTNRQRRPTATATLRDSISEVDA
                     DDFSVRLLGRRSLGPAGLRVGVDVNGRTGLEARNVTNDYDVGGNPTDSTFEVAVADAS
                     RTDFGAFVEGDLPLAGDRLTLTAGLRGDHVRTENAGGYFGDRSTSDGALSGFATLTWA
                     LAPEWKLSAQYARGFRDPTLSDRYFRGVSGRGFVVGNPDLSAETSNQLDLSLRGRRGA
                     FGFGVSGYVYRIEDLVERYRAGADYGFRNRGETEIAGAELEADVRITPRLVVRLLGSL
                     SQGRILDDDTWAADVLPPTAQLIVDHEPFAGFFWRARLAAMARDERPGASEVVVAGHA
                     RLDLAAGYRLSDLLTITVSSRNVLDARYADSADENNVLAPGRGAVLSLSGSF"
     gene            10885..12132
                     /locus_tag="P5164_03255"
     CDS             10885..12132
                     /locus_tag="P5164_03255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018655990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY42944.1"
                     /translation="MTLLRRPRALYAAFDRFPTRKGASTHIARFAPALFTEYDGGLLY
                     VLGDERLPAHQVEGDVEIVRYSVPVENFLERAVAFGAHLSRLLDEAGDTLEVAHFRDP
                     WSGVPILERERRYATVFEVNALPSVELPYTYPAIAPRTLEKVRASERFCLDAADRVVT
                     PSRTTRDFLVSEGVPAAKVAVVPNGADPVPTRPRPFEAPDAYLIYFGALQPWQGVDTL
                     LRAFARLADLSELRLVVCGSARSRPARAAEKLAEKLGLGERVLWRWELPTTELEPWLA
                     NALLSVAPLRATERNVVQGCAPLKVLESLAAGVPVVASDLPPVREIVTDGVEGRLVPP
                     DRPADLARTIRLLLHLPEERERMAEAARARAARDFGWDRSVALLKDVYRALRPSAAAS
                     SAPRPTEEEPDVRSAEGPKVAVA"
     gene            12092..12967
                     /locus_tag="P5164_03260"
     CDS             12092..12967
                     /locus_tag="P5164_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42945.1"
                     /translation="MFEALKARRSLSPKQRRLLETKQLAGEYEPGALVALLKPIAEYD
                     RLSDKARTPMGCTTGALFVLAFVLLIVSANVSVYLLPLPLAALGAGIALLVTVLKMKK
                     LDLSNNFRQVAMPLFAVLREDMEPGATMNVRLDLSPPTAKEKKTGTGKPYVVPGYRKV
                     VDTTYVDPWFGGGARLADGSVLQWTVTDDVVASERTKRTARGKIKTKTKHRKLTTIAV
                     DVALPKKEYAVAAMPADEGEKVKVKDGEKRTSLHVEKRVKTKSLDPIDPGAVFDAIAE
                     GYRRARPAGAEGSAS"
     gene            12964..13344
                     /locus_tag="P5164_03265"
     CDS             12964..13344
                     /locus_tag="P5164_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42946.1"
                     /translation="MSGCECKGGFFTLRDCDGAPAGACSECGRTACTRHLSPASGFTQ
                     CLDCHARAEQQPREGQNATGEEALDDEWTYGARHRFYTAGYAPLYTGSRHSHYYDGYD
                     TRSFDDEGVPEAETDEGGAGFGDS"
     gene            13346..14509
                     /locus_tag="P5164_03270"
     CDS             13346..14509
                     /locus_tag="P5164_03270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY42947.1"
                     /translation="MRPLVLTENYPPDRGGMAQSCDRIVRSLRAAGLDVDVAHLGRRA
                     VRPLVERQERGLLLAAPADEDPAHATQLLWNEVARLPHRPSHVVAFGGAAPVLAAPAF
                     AAWLGVPLVTLLRGNDFDTGLFSLRRGWALRDALGRSAAVATVSRDHQRKVAALFPAA
                     DVRYVPNGIDPAEWSLAPHDLDRAVRWRAEHVGEGRRVLGMFGHLKRKKGGAFLLDAL
                     RRAGVEGKLHLLVVGEAEPEMLAHLASIESTVPFTHVPFVDRFELLPWYAACDGVVVP
                     SLYDGLPNVLLEAGALGVPAVASTAGGMADLLESVEAGLTFPPGDLHECRRALAAFAT
                     LPDGKLRTMGERLRGTILRGFRHDQEAARYRALLAETAGRVPRPAPADVLTES"
     gene            14513..15124
                     /locus_tag="P5164_03275"
     CDS             14513..15124
                     /locus_tag="P5164_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42948.1"
                     /translation="MKSPLARLLPLVLLAVPLAAGAVTPAPADVNTVVVRSAGGSKSF
                     TVIEKGNAIEIVLPGGVRLLGEPAGEKRKYRRGAGGPPVCEVKPGDAGFKLRTTDGKL
                     LWKVKLADDKIKVSDNEENANPYSIKTKYPDKAKVVDAAEKELAEVRFGPDKTKVKGA
                     DGAELWVTESPRRRTASFGVLVLERIPEEHRAILMAEILARRR"
     gene            15132..15986
                     /locus_tag="P5164_03280"
     CDS             15132..15986
                     /locus_tag="P5164_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42949.1"
                     /translation="MLLYDALGGGLGHLVRARAFLHTLGLEEEATLFTSSRFAGDPRV
                     TGGLPVVRVPSSLDGDRAGLRALLEETLAETRATRLVVDAFPAGLLGELAEWAPPPGL
                     VLWHVARLLRWERYTSDASPRLPRFDVTFVVEELHAEHRRALEAASRGLRPLDLVDPP
                     APPPEPIEGRHVLVVHSGPDEETLDLVACARELATADGHGARVVLASPARPGSLPVDV
                     EWRDVFPATGLFPAADRIVAAAGFNAVRQTEPFRAKRFLVPYPRRFDDPFARAARGRC
                     PARGRCQA"
     gene            16142..>16501
                     /locus_tag="P5164_03285"
     CDS             16142..>16501
                     /locus_tag="P5164_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007333524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HRY42950.1"
                     /translation="MSGTIDSIVQAARVLADPSARARFLDEACRGDEAARARVDAILQ
                     ADDPTVDSLPAPDALSSDDTVAPGEAGARAPLFEGPGTVLGPYRLIERIGQGGFGTVF
                     LAEQQEPIVRRVAVKLLK"
BASE COUNT         2096 a         6406 c         5760 g         2239 t
ORIGIN      
        1 gaggtcctcc gcgtcgagtc gggcaccttc tccgacacgc acgcgatcgc ggtcttcctc
       61 gccatgaagg acaacgagtg gctcaccccc gcccgcttcg agccggggac gctcgtgaag
      121 gtgcggctcg tcccgttcaa ggccgcgccg tccgccgtcc gctcgatccg gcgcgtcgac
      181 gacctcgacg acttcgagcg catcccgtac tacgtcctcg attgggagat ccgatgaagt
      241 cctggccccg cctcgccccc ggtctcgtcc tcggcctcct cctcgccgca cctaccgcgg
      301 cggacgcagc gaccccgccc gccgctcccc gaaaggcgcg ctcggcgcgc cccccggccg
      361 cgaccgctcc ctcgcgcgcg gagagcatcg ccgccgcgcg ccggctcctc ctcggccagc
      421 tcgcggcgtc gggggccgga tcgtgggacg cctggagcgc gtcggtcgag ccgttccgcg
      481 cctcgctccg cgagcgggtc gcggcggcga agccgaacaa cccggattcg aagggctaca
      541 cggagtcgcg ctacgaggtc ctcgagtcgc tcggcgagcc gccgcggttc gaggcgtggc
      601 cgtcgagcta cgtgaggtac gtcgcgaacg ccccggtctc gacgtcggag ttcctcaccc
      661 gcaccgcctt cgtctcctcg atcgaggcca tctcgcgctg gctcgcctcc cgcggcatcg
      721 acttcatctt cgttcccgtg ccgaagatga cggaggtcta ccccgaggcg ttcgtcgcgg
      781 cggcgccggc cgaccgcatc gtcgccccgc agatgaggcg ggcgatcttc gagctcctcg
      841 agcggaacgt cgaggtcgtc gacctcctcc cgctcttcct cgaggcgcgc acacgcccgg
      901 gcgcgccgtc gctctaccag cacgccgacc cgcactgggc ctgggaggcg cagaagatcg
      961 ccctggagga gatcgtccgg cggctccggc gctaccccga ggtccggaag tcgctcgccg
     1021 ctccccgccg cttccggacc tggaccgaac ggcgcgatct cacgtcggtc tccgcggcgt
     1081 tcggggcgct cacgccggag caggcccggc gggtgaagcc gttcctgacg gcgaacgtcg
     1141 agttcgccgc gcccctcggc gaaaccccgc tcgcgtccga agactcgccc atcgcgctcg
     1201 ccggggacag ctacaacaac ggactggaag ggggcctcgc cgcccgcctc aacatgccga
     1261 tccgcgacct ccacgcctcg ggcgggtcgc tcgccccctt caaggacctc ctgcgcgacc
     1321 cggagtcgat gaagtcgatg aaggtcctca tcttcctcgt caacaaccag gcgctctggg
     1381 agttcccggc gacgctcccc ggagagatcg cgaagttcca ggtctcgcgc cccccggact
     1441 gacctcaggt cgacgacgga ggccgctcct cctcagcctt tcaggcccgg gtcgcgccgc
     1501 gccgcgagcg cgaccgtgag cgcgaggagg acgagccctc ccgccgcgcc gagcgcgacg
     1561 gacaggacca cggcctcctc cttcccgtgg agcgcgtgga gcggcatgag ccacgggtag
     1621 tacggcccca gcctcgagcc ggacgcgaag agggcgagga acgtccccgc gacgccggtg
     1681 ccgagcgaga cgacgatcga cgggaagcgg aggctgacgg cgttctggat cgcgaggacg
     1741 aggagcgcgg cgccgaagac gcgggccgtc cgtccgagca tcgtcgcgac cggagccgcc
     1801 gtctcgaggg cgatcttcgg gtggagggcg tcggcgagga ccgcaccccc gaggcacccg
     1861 aggacgagga cgcccgtggc gaggaggagg agccccgtcg cgacgagcgc cttcgccgcc
     1921 ggcacgaccc agcgcggcag cgggaggacg ccgaggcgca gccacccctg gttcgcgtgc
     1981 tcggcgtggg agacgagagc gacgacgagc gccgtcgcga gggggagcat gaagaccccc
     2041 cacatcgaga gcgcgttctt gtggaacgcg agccagggct cgacgtccgc tccgaacccc
     2101 tccttcatcc ggagccagat cgccacctgc aacgcgacga cgacgagggg cgcgacgaag
     2161 caggtcgcga gcgcgagggt ccgcttcagc ttcaggagct cgatccggac ggcgacggcg
     2221 aggcccctca cgcggcggct ccttccggga cgcccgccac gagcgcgagg aaggcgcgct
     2281 cgaggctcgc ctcctcgagg gcgaggtggg agaccgcgag cccctccccg acgaggagag
     2341 tgttcacccg ggcgatcccc tcgcggccgg ccacgcccac cgcgaggagc ccgtcgggcc
     2401 ggagcgtcgc ggagagatcc acccgccgga ggagcgcctg cgcccgcgcc gggtcgtcca
     2461 cgcggacgcc gaggacgccc ggctgcgtcc ccttcagctc ctcgagcggc ccctggaacc
     2521 ggagccgacc gcgcgcgagg acgccgatcc acgtcgcgac cgactcgagc tccgcgagga
     2581 tgtggctcga gacgaggacg gtgattccct cctcggcggc gagccgtcgg atcgtctcgc
     2641 ggagctcgcg gacccccgcc gggtcgagcc cgttcgacgg ctcgtcgagg acgaggaggc
     2701 tcggggcgtg gaggagcgcg agcgcgaggc cgagccgctg cttcatcccg agcgagaagc
     2761 ggcggacgag cttcttcccc tcgcccgcga ggccgaccgc cgcgagcgcc cgctccacgt
     2821 tcgcgcgcgg gaccccgcga gcgagctggt cgagccgcag gttctcctcg gccgtcaggt
     2881 ggggatagag cgccggcgac tcgacgagcg ccccgacgcg cgcgagggcg gccgaccgcc
     2941 ggccgttcac cggctcgccg aggagctcga ccgaccctcc cgagggcgtc aggaggccga
     3001 ggaggagctt gatcgtcgtc gtcttccccg ccccgttcgg cccgacgaag ccgtagacgc
     3061 tcccgcgcgg tacggcgagg tcgacgcccg agacgacggc cgtctccccg tagcggtagg
     3121 tcaggccgca cgtcgtcacg acgctctcca tctactcctc ctcgacccgc cggctcggcg
     3181 ccgccgtcct caggaacacg aatgcgtcga agagccgggg ccacggcgcg gtcatcgtcg
     3241 cctggcccat cggccgggcc ttcaccggga gctcgagcca gctccccggc gcgtttcggc
     3301 ggagatccag gaagagcgcc ggcaggcccg ttcgctcggc gtggtcctcg aagcttccgc
     3361 ggcgcggcgg cgcgaggtcc cagggcgagg agaagaccga cccgacgcgc cccgcccccg
     3421 acgtgaaggc gacgacgtag gcgccctctc cgagctcgtc ccgcaggcgc tggcccatcg
     3481 gcaccatctc cgggtccggg aacgggcgct cgacgaggtc gcgccggcgg gaggcgtgcg
     3541 acgtcgcggc ccaggcgacg accttccgtc cccggtagcg ctccgacatc agccacagca
     3601 ggttctcggc catctgcgcg tcgcggcggt tcatcacctc gggagccggc ttctcgaagt
     3661 cgacgtccca gaggaagcgg gcgtcgaccg cggtgctcct cacgacctgc gcgaggaacg
     3721 ccccctccgc gtcggcccgc gcggcgaggc gccgctcgag ctccgccgcc gcggcggcga
     3781 acgcctcccg ctcctttgcg ggcgccttcc ggaacccctt cgccgggctc tcgaggagct
     3841 tcccgagcgg cccgaagaag gcctcgagct cgcccggcgc gagccccgcc gcggcccgga
     3901 gggccgggga gagctcctcc cggctgatcc ggccggtcag ctgcatgtcg aagcccgtca
     3961 gctcgagcgg ggcgcgcgtc cgcgcagagg cgccgaggtg ctcgaccatc ggccggaact
     4021 cctcgctctc ggcccagacc tcgaagaccg agccgcgcac cgcctcgcgc gccggcgcgc
     4081 cgcggaggat cgcgtcgccc gcccgctcgc acgcgaagag cccgctctcc atcgcgagga
     4141 cgtcgaaccc catccgctcg tggaggaagc ggacgagccg cgtcttcgcg aggaacgtgg
     4201 agccctcgcc gtgcgtctgc tcgccgagga ggacgagccg ggcgtcgccg atcgcggcgc
     4261 ggagcggctc gaggtcgtcg agcgacgcct ctgcggggtc gatcccccgg agcgggacgg
     4321 cccgcgcttc tatccagcgc gactcgggcg tcggcgcgag gtcgcgcccc cggcagcccg
     4381 cggccgccgc gagggcgagg agccccgcgc atacgaacgc ccgtcgcgcc ctccggggcg
     4441 cgccccgtcc cgtcgtgtcc ggcacggcag gtaatacgga cgcgccgccc ggcggttaac
     4501 gggagcgtcg ggacggccga gatccctccg tcccggagcg cagagcgtgg acgacagccc
     4561 tggccgctgg ttctcgacgg tttcggatac tgtcccggaa tgcaggacct cgtcggcggc
     4621 ttcctcgccg cgcctctcgc ggtgcaggcc gttctggtgt gcttcgccct cgccttcctc
     4681 gcgatggtct tcgcccgccc cctggcccac cggagggcgc gcgagcggtt cgccgccctg
     4741 gccgcatcga tgggcgcgcc ggtcgctcct ggccgcagcc cgctcgaggc ttcgttcggg
     4801 gccgtctgcg aaggacgccg cttcgaggtc cggcgcgagc tgcggagcgg catcgccggc
     4861 agccactacc gcggaccccg tggcaacctc gtcgtcctcg agacgccgct ctccggcagc
     4921 cgctgggaga tgcacggggt ggagatcgtc ccgggagggc cccggaagcg ccccggcgcc
     4981 cgcgcgctgg agaccggcga ccccgatttc gacgcgcgct tcgccgtctg gcaggacggt
     5041 gtgccggtgc gcgagggctg gctcgacgcc ccgacccgcg ccgcggtcac ggcgttcttc
     5101 gacggccccg gcgtcgccgg cccggtctgg gcgcaggagg ggcggctgac gtacgtcgcc
     5161 gtgccgcccg ccaggctcga cgagccgaag ctcacggcca tcctgcgccg gcaggccgct
     5221 ctcgcctcgg ctctcgagcg aaccgccggc tggaggggac cggccgcgta accgcggcgc
     5281 gggaggggcg cgggcccgcc gcgcccctcc cgcctcagag cgccagcgtc ttcgcccccg
     5341 ccgcgagcgt cgccttcacg acctcgagga gcttcgcggg gggcacgccc tcggccttct
     5401 tctcgcggcg cgtcgagacc tcgacgaccc ccttcgcgag cgccttgccg ccgaccacga
     5461 cgcggacggg aaagccgatc aggtcggcgt ccttgaactt cacgcccggg cgttcgtcgc
     5521 ggtcgtccca gaagacctcg acgcccgcct cggtcagctc cgcgtagatc ttctcggcgg
     5581 cctccaggac ctccttgtcg cgctggagcg agaggagggc gacctggaac ggcgcgagcg
     5641 ggacgggcca gatgatcccg tcgtcgtcgt ggttctgctc gacggcggcg gcgaccgtcc
     5701 ggccgacgcc gaggccgtag cagcccatct ggagcgggac catcttctgc tgcgggtcgg
     5761 ccacctcgca cttcatcgcc tcggagtact tcgtcccgag cttgaagatg tgccccacct
     5821 cgatgccgcg cttgatccgc aggaccccgg cgccggtcgg cgacgggtcg ccgtcgcgcg
     5881 ccgtcgccac ctcgatcacc ccggccacct tcaggtcgcg cgggacggag aggttggagt
     5941 ggtgggtgcc gtcctcgttc gctccgcaga agacgttccg ggcattctcc atggagcgat
     6001 cgacgtagac ggggacctcg tcctgcccct tgcgcggctt caggccgatc gggccgatga
     6061 agcccggctt gcagccggcg atctcgggga gccactcctc ggggacgagg cggacgtgcg
     6121 cgacgccgag cgccttcggg agcttcacct cgttgatcgt caggtcgccc cggatgacga
     6181 tcgcgcagac gaacgagctg tcgtcggcgc ggatcgcctc gtagagcatc gtgttcgtga
     6241 tccggtcttc cgtcgtcccg aggtgcttcg cctgcccctc ggtcgagacg atccccggcg
     6301 tcggggagtc ggcccggccg aacggcttct cctcctcgtt cgcccagggc tcggggagcg
     6361 gcgccgtgac ggccttctcg acgttcgcgc cgtacttgcc gtcctcggag aagacgagcg
     6421 cgtcctcgcc agtgtcggcg acgaccatga actcctcgga cgcggagccg ccgatggcgc
     6481 ccgagtcggc ctggacgagc gcgtactcga ggccgcagcg ctcgaagatc gcgcggtagg
     6541 cggcgtccat cttccggtag tgggcgtcga gccccttcgg gtcgacgtcg aaggagtagg
     6601 cgtccttcat caggaactcg cgaccgcgca tcagtccgaa gcgcggacgg atctcgtcgc
     6661 ggaacttcgt gcggatctgg tagaggttga acggcagctg ccgccacgac gtgaccgacc
     6721 ggcggaccat gtcggtcacc gcctcctcgg ccgtcggcgc gagggcgaac tcggtcgcct
     6781 tgcggtcctc gaggtggaag aggatgccgt cgttcatgta gcggtcccac cgccccgact
     6841 ccacccagag ctccttcggc tgcgcgatcg ggagctccac ctcgagcgcg ccggtccggt
     6901 tcatctcctc ccggacgatc tcggtcatct tcttgagcga ccggaagccg agcggcgtga
     6961 aggaatagat gccggcggcg gccttcagga gcatccccgc gcgggcgagg agcttgtggg
     7021 agacgacctc ggcatcgccg ggggcctcgc ggagggtgga gaggaagtag ttggaccagc
     7081 gcatcggggg cggagaatag cgcagcggag cgggttccgt tcccggtacg atcgccctcc
     7141 ggcccgggcg gacgccccga ggccgcggga ggcccctgga tggacgcccg gccgttcgac
     7201 ttcgttcccg tcgcctcgcc tcttcacgac gccgcggcgc tccggcgcgc gctcgagccg
     7261 tacctcgggg ccctcgtcgg cctcgggggc cgcgagacgg ggccggaagc ggcctccggc
     7321 gccggccccc tcgcgatcgt cgtcgggacg ggaggcaccg aggccgccgt tctccgcctc
     7381 cgcgaagccc ggcaggcggc cgcgcccggc gagccgctcc tcctcgtcac cctcccggcc
     7441 cacaactccc tcccggccgc cctggaggcg ctcgcgcgcg tgaggcagga cggcggtcgc
     7501 ggccggatcg tcccgctgca cggccccgac gacgggcccg gcctcgttcg cctcgcggcc
     7561 gccctcgagg acctgcgggt gcggaagcag ctccaccggg cccggatcgg cctcgtcggg
     7621 acgccgtcgg actggctcgt ggcaagcgtc cccgctgccg cagccgtccg cgagacgtgg
     7681 ggccccgagg tcgtcccggc cgacctcgct cccctcctcg aagccccgct ccgcccgccc
     7741 gacgaggcgg ccgccggcct cgccgcctcc tacacctccg ggccggcctc cgtccgcgag
     7801 accgacgagg cgggcgtgaa gaccgccgcg agcgtcctcc cggcgctcgc ggacctcgcc
     7861 gcgcgcgagc ggctcgacgc cgtcgccgtc cgctgcttcg acctcgtcac cgagcgagga
     7921 acctccggct gcctcgccct ctccgccctc aacgactccg gcaccgtcgc cgcctgcgag
     7981 ggggacctcg cgacggccgt cgcgatgctc tgggtgaagg agcttctcgg caccgcctcc
     8041 tggatggcga acccctccga cgccgacgcc gtgaccggcc gcctccgcct cgcccactgc
     8101 accgtccccc gcgccctcac ctccggctgg gacctccgct cccacttcga gtcgggcctc
     8161 ggcgtcgcgc tcgccggaga gctcccgccc gggcccgtca ccctcctgcg cctcggcggc
     8221 cgccgcctcg agtcggtctt cctcgccgag ggcgaggcgc tcccgacgcc ccgccgcgag
     8281 gacctctgcc gcacgcagct cgacgtcctc ctcgagcccg acgccgtcgc cgagctcctc
     8341 gaacgccccc tcggcaacca cctcgtcctc gtgccggggc acgtcgccgc gcgactgcgg
     8401 gcgtaccgcg agtgggcggt gggaggggaa tcgcgggagg gctgaggggg gatcggccac
     8461 gggcggcggc ggtggcgcgc ggccgcacgt cgcccggccc gcggggcgcc ttgccgcccc
     8521 tgtcccgccc ccgtactctc cggcgcgacg atgcgtctcc tccgcgccgc gcttctcgca
     8581 ccgctcgccc tcctcgccct tctcgccgcc ccccggctcg aggcggcgcg ttaccgcgtc
     8641 gtcgacgaga acggccgcgc cgtcccgggg gcgcgcgtct cgatcctcgg ccgcgccggc
     8701 tccgtcgccg cggggccgga gggcgagctg acgctcgagg ccgagccggc gctcccgttc
     8761 gaggtcggcg tcttctcgcc gaccggcgcc tggctcggga tcgtccgcgt cgagacgcga
     8821 gcgagcggcg acggggtccg cgacctcgtc ctccccgcgg cccgcagcac cgaggtcctc
     8881 gtctccgcgg gcgccgccgc ctcgctcctc gcccctcccg cgaacccggt ctcgctcgtc
     8941 tcgaagcggg agctcgagga gcggcggccg aaccgcctcg tggaagccat cgagacgctc
     9001 cccggcatct cgaagagcga cgagtcggcg agcgccgtgc cggtcatccg gggcctcgcc
     9061 cgcggccgaa cgctcgtgat ggtcgacgac ggccgcgtct ccgccgagcg gcgcgccggc
     9121 gcctccgcgt cgtacctcaa tccgttcgcg ctcgagtcgg tcgaggtcgt ccgcggcccc
     9181 ggctccgtgg cctacggctc cgatgccctc ggcgggatcg tccacgcccg gacgccgctt
     9241 cccgagccgg gcgtcttccg cgcccgctac cagctcggcg gcggcgtcgg ggggagcccc
     9301 gaggcctccg ccggcgtcgg tctcaatgtc cctctcggcc cggccgcctt cctcgtccac
     9361 ctctaccagc ggagcgcggg cgactacgac tcgcccgccg gcgagcagga caactccgcg
     9421 gtgagggacc gcggcttcct cgtccgcggc gcgatccctc tcgggaaggc gcgccttttc
     9481 gccggcttcc agctcgacgc ggtgcgcgac atgggaaagc cgcaggccga cctcccgacg
     9541 acgcggacgt tctacccgtg ggaagactcg cgccgcttct cgctcggcct cgacgcgccc
     9601 gacgtcgccg gcttcacctc cgtcgagctg cgcgcgttct acggctccta cgggatctcg
     9661 acgaaccgcc agcgccggcc gaccgcgacg gcgacgctcc gcgactccat ctccgaggtc
     9721 gacgccgacg acttctccgt ccgcctcctc ggccgccgct cgctcgggcc ggcagggctc
     9781 cgcgtcggcg tcgacgtgaa cggccggacg ggcctcgagg cccggaacgt gacgaacgac
     9841 tacgacgtcg gcgggaatcc gacggactcg acgttcgagg tcgccgtcgc ggacgcctcg
     9901 cgcaccgact tcggcgcctt cgtggaaggg gacctcccgc tcgcggggga tcgcctgacg
     9961 ctgacggccg gccttcgcgg cgaccacgtg aggacggaga acgcgggcgg gtacttcgga
    10021 gaccgctcga cctccgacgg ggcgctctcc ggattcgcga cgctcacctg ggcgctggct
    10081 cccgagtgga agctctccgc gcagtacgcg cgcggcttcc gcgacccgac gctctccgac
    10141 cggtacttcc ggggtgtctc cggccgtggg ttcgtcgtcg gcaaccccga cctctccgcc
    10201 gagacctcga accagctcga cctctcgctt cggggacgcc ggggcgcctt cggtttcggc
    10261 gtctccggct acgtctaccg gatcgaggac ctcgtcgagc gctaccgcgc cggcgccgac
    10321 tacgggttcc ggaaccgcgg cgagaccgag atcgcgggag cggagctgga ggccgacgtg
    10381 aggatcactc cgcgcctcgt cgtccggctg ctcggctcgc tctcccaggg acgcatcctc
    10441 gacgacgaca cctgggccgc cgacgtcctc ccgccgacgg cgcagctgat cgtcgaccac
    10501 gagccgttcg ccggcttctt ctggcgggcg cggctcgccg cgatggcgcg cgacgagcgg
    10561 cccggcgcga gcgaggtcgt cgtggccggg cacgcccggc tcgacctcgc cgcgggatac
    10621 cgcctctccg acctcctcac gatcaccgtc tcgagccgga acgtcctcga cgcgcgctac
    10681 gccgactcgg ccgacgagaa caacgtcctc gcgccgggtc gcggcgcggt cctctccctc
    10741 tcgggctcgt tctagcccgt ccgtctccgg gaccgggtcc ctcgcggctc cgcgagggac
    10801 ccctcccgga ccggccgcac cgccccgctc gtcgaaatct tgcgcgaagg gctcgccggt
    10861 ccctatcctc cgcaccacat tccgatgact ctcctccgcc gaccgcgcgc gctctacgcg
    10921 gcgttcgacc gcttcccgac ccggaagggg gcgtcgacgc acatcgcccg gttcgcgccg
    10981 gcgctcttta cggagtacga cggaggcctg ctctatgtcc tcggcgacga gcggctcccg
    11041 gcgcaccagg tcgagggaga cgtcgagatc gtccgctact ccgtcccggt cgagaacttc
    11101 ctggagcggg ccgtcgcgtt cggcgcgcac ctctcgcgcc tcctcgacga ggcgggcgac
    11161 acgctcgagg tcgcccactt ccgcgacccg tggtcgggcg tcccgatcct ggaacgggag
    11221 cggcggtacg ccacggtctt cgaggtgaac gcgctcccct ccgtcgagct gccgtacaca
    11281 tatcccgcca tcgcgccgcg gacgctcgag aaggtccgtg cctctgagcg cttctgcctc
    11341 gacgccgccg accgcgtcgt cacgccgtcg cggacgacgc gcgacttcct cgtctcggaa
    11401 ggcgtcccgg cggcgaaggt cgccgtcgtc ccgaacgggg ccgacccggt cccgacgcgg
    11461 ccccgcccgt tcgaggcgcc cgacgcgtac ctgatctact tcggcgcgct ccagccgtgg
    11521 cagggcgtcg acacgctcct ccgcgccttc gcccgcctcg ccgacctttc ggagctgcgc
    11581 ctcgtcgtct gcgggtcggc gcggtcgcgg ccggcccgcg ccgcggagaa gctcgcggag
    11641 aagctcggcc tcggcgagcg ggtcctctgg cggtgggagc tcccgacgac cgagctcgag
    11701 ccctggctcg cgaacgcgct cctctccgtc gcgccgcttc gggccacgga gcggaacgtc
    11761 gtccaggggt gcgccccgct gaaggtcctc gagtcgctcg cggcgggcgt ccccgtcgtc
    11821 gcgtccgacc tgccgcccgt ccgcgagatc gtgacggacg gcgtcgaggg gcggctcgtc
    11881 cctcccgacc ggccggccga cctcgcgcgg acgatccgcc tcctcctcca cctccccgag
    11941 gagagggagc ggatggcgga ggctgcccgc gcacgcgccg cgcgcgactt cggctgggac
    12001 cgctccgtcg cgctcctgaa ggacgtctac cgcgcgctgc ggccctccgc cgcggcctct
    12061 tccgctcccc gcccgaccga ggaggaaccg gatgttcgaa gcgctgaagg cccgaaggtc
    12121 gctgtcgcct aagcagcgac ggctgctcga gacgaagcag ctcgccggcg agtacgagcc
    12181 gggcgcgctc gtcgcgctgc tgaagccgat cgccgagtac gaccggctga gcgacaaggc
    12241 gcggacgccg atgggctgca cgacgggagc cctcttcgtc ctcgcgttcg tcctcctgat
    12301 cgtctcggcg aacgtctccg tctacctcct gccgctcccg ctcgcggccc tcggcgcggg
    12361 gatcgcgctc ctcgtcacgg tcctgaagat gaagaagctc gacctctcga acaacttccg
    12421 gcaggtcgcg atgccgctct tcgccgtcct ccgggaggac atggagccgg gcgccacgat
    12481 gaacgtgcgc ctcgacctct cgcccccaac ggcgaaggaa aagaagaccg gcaccgggaa
    12541 gccgtacgtc gtccccggct accgcaaggt cgtcgacacg acgtacgtgg acccgtggtt
    12601 cggcgggggg gcgcggctcg ccgacgggag cgtcctccag tggacggtca ccgacgacgt
    12661 cgtcgcgagc gagaggacga agcggacggc ccgcgggaag atcaagacga agacgaagca
    12721 ccgcaagctg acgacgatcg ccgtcgacgt ggcgctcccg aagaaggagt acgcggtggc
    12781 ggcgatgccg gccgacgagg gggagaaggt gaaggtgaag gacggcgaga agcggacttc
    12841 gctccacgtc gagaagaggg tgaagacgaa gtcgctcgac ccgatcgacc cgggggcggt
    12901 cttcgacgcg atcgccgagg gctaccggcg cgcccgcccg gcgggagcgg aggggagcgc
    12961 gtcatgagcg gctgcgagtg caaggggggc ttcttcacgc tccgggactg cgacggggcg
    13021 cccgcgggcg cctgctcgga gtgcgggcgc acggcctgca cccgccacct ctcccccgcg
    13081 agcggcttca cgcagtgcct ggactgccac gcgcgcgccg agcagcagcc gcgggagggg
    13141 cagaacgcga ccggggagga ggcgctcgac gacgagtgga cctacggcgc gcggcaccgc
    13201 ttctacacgg ccggctacgc ccctctctat accggctccc gccacagcca ttactacgac
    13261 ggctacgaca cccgctcgtt cgacgacgag ggggtcccgg aggccgagac cgacgaaggc
    13321 ggggcgggct tcggggacag ctgacgtgcg gccgctcgtc ctgaccgaga actacccgcc
    13381 cgaccgcggg gggatggcgc agtcgtgcga ccggatcgtg aggtcgctcc gcgcggccgg
    13441 tctcgacgtc gacgtcgcgc acctcggccg gcgcgccgtg cgcccgctcg tcgagcggca
    13501 ggagcgcggc ctcctcctcg cggctcccgc cgacgaggat cccgcgcacg cgacgcagct
    13561 cctctggaac gaggtcgccc gtctgccgca ccgcccgagt cacgtcgtcg cgttcggagg
    13621 cgccgctccc gtcctcgccg cgcccgcctt cgcggcctgg ctgggcgtcc cgctcgtgac
    13681 gctcctgcgc gggaacgact tcgacaccgg gctcttctcg ctccggcgcg gctgggctct
    13741 ccgcgacgcg ctcgggcgct cggccgcggt cgccaccgtc tcgcgcgacc accagcgaaa
    13801 ggtcgccgcg ctcttccccg ccgcggacgt ccggtacgtc ccgaacggca tcgacccggc
    13861 ggagtggtcg ctcgcaccgc acgacctcga ccgcgcggtg cgctggaggg cggagcacgt
    13921 cggagaggga cggcgggtcc tcgggatgtt cgggcacctg aaacggaaga aggggggcgc
    13981 cttcctcctc gacgccctcc ggcgcgcggg agtcgaaggg aagctccacc tcctggtcgt
    14041 cggcgaggcc gagccggaga tgctcgcgca tctcgcgtcg atcgagtcga cggtgccgtt
    14101 cacgcacgtc ccgttcgtcg accgcttcga gctcctcccc tggtacgccg cctgcgacgg
    14161 cgtcgtcgtc ccgtcgctct acgacggcct cccgaacgtc ctcctcgagg ccggggcgct
    14221 cggcgtcccc gccgtcgcct cgacggcggg cgggatggcc gacctcctcg agagcgtcga
    14281 ggccgggctc acgtttccgc ccggcgacct ccacgagtgc cggcgtgcgc tcgccgcgtt
    14341 cgcgacgctc ccggacggga agctgcgcac gatgggcgaa cggctccggg gcacgatcct
    14401 ccgcggcttc cgccacgacc aggaagcggc gcgctaccgc gcgctcctcg ccgaaacagc
    14461 cggccgcgtc ccgcgcccgg ctcccgccga cgtcctcacg gagagctgaa ccatgaagag
    14521 tcctctcgcc cgcctcctcc ccctcgtcct tctcgccgtt ccgctcgcgg ccggggcggt
    14581 gacgcccgcg cccgcggacg tgaacaccgt cgtcgtccgc tcggccggag gctcgaagag
    14641 cttcaccgtc atcgagaagg ggaatgccat cgagatcgtc ctcccgggcg gcgtccggct
    14701 cctcggcgag ccggccgggg agaagcggaa gtaccgccgc ggcgcgggcg gcccgccggt
    14761 ctgcgaggtg aagccgggag acgccggctt caagctccgg acgaccgacg ggaagctcct
    14821 ctggaaggtg aagctcgccg acgacaagat caaggtgagc gacaacgagg agaacgcgaa
    14881 cccgtactcg atcaagacga agtaccccga caaggcgaag gtcgtcgacg ccgccgagaa
    14941 ggagctcgcc gaggttcgct tcggccccga caagacgaag gtgaaggggg ccgacggcgc
    15001 ggagctctgg gtgacggaat cccccaggcg acggacggcg tcgttcggcg tcctcgtcct
    15061 cgagaggatt cccgaggagc accgggcgat cctgatggcg gagatcctcg cccgccgccg
    15121 ctgaggcgcg cgtgctcctc tacgacgcgc tcgggggagg gctcgggcac ctcgtgaggg
    15181 cccgggcctt tctccacacg ctcgggctcg aggaggaggc gacgctcttc acctcctcgc
    15241 gcttcgcggg cgacccgcgc gtgacgggag ggctccccgt cgtgcgcgtc ccttcgtcgc
    15301 tcgacggcga tcgagccggc ctgcgtgcgt tgctcgagga gacgctcgcg gagactcgcg
    15361 cgacgcgcct cgtcgtcgac gccttccccg ccggcctcct cggcgagctc gcggagtggg
    15421 ctccgccgcc cggcctcgtc ctctggcacg tcgcgcgcct ccttcgctgg gagcgctaca
    15481 cctccgacgc ctcgccccgc ctcccgcgct tcgacgtgac gttcgtcgtc gaggagctcc
    15541 acgccgagca ccgccgggcg ctcgaagcgg cctcccgtgg gctccgcccg ctcgacctcg
    15601 tcgacccgcc ggcccccccg cccgagccga tcgagggccg gcacgtcctc gtcgtccact
    15661 cggggcccga cgaggagacg ctcgacctcg tcgcctgcgc ccgcgagctc gccactgcgg
    15721 acggccacgg ggcccgcgtc gtcctcgcct cgcccgcccg gcccggctcc ctccccgtcg
    15781 acgtcgagtg gcgcgacgtc tttcccgcaa ccggcctctt ccccgccgcc gaccggatcg
    15841 tcgccgcagc cggcttcaac gccgtgaggc agacggagcc gttccgcgcg aagcgtttcc
    15901 tcgtccccta cccccgccgc ttcgacgacc cgttcgcccg cgccgcccgg ggtcggtgcc
    15961 ccgcccgggg tcggtgccag gcgtgaaatc gtgtattgtt aaaaacaata cacgatttca
    16021 cgcctggcac cacgcctcgg caccccgcct cttggcaccg cacttctgag ccgcggcccc
    16081 gccgtcttcg tcttcgcgcc ccccctgctc tatcctcccg accgaacgat ccggaggaac
    16141 ggtgtcggga acgatcgact ccatcgtgca ggcggcgcgt gtgctggccg atccgtcggc
    16201 tcgcgcgcgc ttcctcgacg aggcctgccg gggcgacgag gcggcgaggg cgcgcgtcga
    16261 cgcgatcctc caggccgacg acccgacggt ggactccctg ccggcgccgg acgcgctgtc
    16321 gtccgacgac acggtcgccc cgggcgaggc cggcgctcgg gcgccgctct tcgaggggcc
    16381 cgggaccgtg ctcggcccct accggctgat cgagaggatc gggcaggggg gcttcggaac
    16441 ggtcttcctc gccgagcagc aggagccgat cgtccgccgc gtcgccgtga agctcctgaa
    16501 g
//
LOCUS       DAOTEZ010000038        16232 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2429_length_16232_cov_6.218211, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000038 DAOTEZ010000000
VERSION     DAOTEZ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 16232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16232
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2429_length_16232_cov_6.218211"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="P5164_03290"
     CDS             complement(<1..905)
                     /locus_tag="P5164_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HRY42951.1"
                     /translation="MNRSLRRLARLSAPFAAGFAALALASPAASQTKLLRFPDVHGER
                     VAFCYGGDVWTAPVSGGLAARVTAHAGQELFPKFSPDGKWIAFTGQYEGDEQVYVVPA
                     EGGVPKRLTWYPSRGPGAPRHGGDNQVYGWTPDGKAVLFRSLRDAESAKTETALYTVP
                     VAGGLPAKLPMYTAGAGDFSPDGKRLVYSPLFRDFRTWKRYEGGWAQDLWIYDLAAGT
                     QRAIAPSRRTERDPMWIGERVFFVSDRDGTLNLYSVGVDGSGLEQLTRSTTWDVRWAS
                     SDNAGKVVYELGGELRLWDVAAKAER"
     gene            complement(1048..3255)
                     /locus_tag="P5164_03295"
     CDS             complement(1048..3255)
                     /locus_tag="P5164_03295"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRY42952.1"
                     /translation="MSPRIGSEPRTFEAGGRPFVLRRDGAGVMHAEAGDDLGLAAAWG
                     FAHAHDRALQLLVTRIVGQGRLCELLDDSPASLEIDLFMRRGLFAAASAEEAASLPVR
                     ARSLTEAYAAGVNARLARGRPWELRLLRVPAEPWTPADTLLTLRLMTFVGLAQAQSDA
                     EVAILQALRAGVDRGKLARLFRPHLDGLDDALLEAIRSLRHVPALVPGLPALPTLRAS
                     NNVAVAGGRSASGAALYASDPHLEVNCLPAIWYEVVGTLPGGDFRTGVTVPGVPGLVM
                     GRTRALAAGFTYGFADTIDLVIEEVRDGRFRRGERWLALRQRRETVRRKKGPPRELVV
                     FETDAGVLETPDGVARVEDGLWIARAWTGRREGGAASLEAILDLWAAPDVDRGADAAA
                     RVALSASWLLAGRDGRIAAQQSGLLPRRRRAPGLLPLRGWEAGDLWDGTLSPPSLARA
                     AGSAEGVLVTANDEGHGAWKPVGITLCMGSDRADRLRELAGAKAAAGGGLSAADLGAM
                     QADLVSGQARRLLSALEPYLPDLPAVRELRAWDRRYDAASKGAVLFERLYAALCEELF
                     GAGLLGRDAWRALRDTTLVHAFFFRLFDDLLAREEGEEWFGAEGKAAFVSRVARRALA
                     GADPSALLPWGASRRVFLRHLLFGGKLPPVFGFDRGPFEVVGGRATVAQGQLLSLAGR
                     ERTICPSWRFVTDLATDVAETALPGGPSDRRFSRWYATDLARWLAFDRKTLKP"
     gene            complement(3297..3587)
                     /locus_tag="P5164_03300"
     CDS             complement(3297..3587)
                     /locus_tag="P5164_03300"
                     /inference="COORDINATES: protein motif:HMM:NF022143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2492 family protein"
                     /protein_id="HRY42953.1"
                     /translation="MTIEAHDHHHDHSHEADGFVFGHEVLRILADRGGRLPVAELKRA
                     AAEAFGADALYGNCHGDRFHFEGLLEFLGSAGKLSLHGEEVSLGRVPGCTGH"
     gene            complement(3661..4767)
                     /locus_tag="P5164_03305"
     CDS             complement(3661..4767)
                     /locus_tag="P5164_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013783636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HRY42954.1"
                     /translation="MRLRAILLPLALLVAPAAGATDGSDLDEAAAGRFARLALDCVHR
                     EYPNKIAHVMNGDADAKPPRELTPAFYGCFDWHSSVHGHWLLARLARLHPEASYTAPA
                     RAALARSLTEGNVAGEVAYLAAPGRTGFERPYGLAWLLQLAAELREWDDPEARAWSRA
                     LAPLEAKAADLLFAWIPKLAYPIREGEHPQTAFAFGLVLDWARGAGETEKAGLLARRI
                     VELYGSDRGCPIGYEPSGQDFLSPCLAEADLMRRVMPSDRYARWLSGLLPDLPRDGSS
                     GWLAPGIVTDRTDGKLVHLDGLNLSRAFMLRGIAASLPPRDRRIPALRAAADAHAKAS
                     LPSVTSEHYEGSHWLGTFAVYLLTERGLPPALPR"
     gene            complement(4900..6744)
                     /locus_tag="P5164_03310"
     CDS             complement(4900..6744)
                     /locus_tag="P5164_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRY42955.1"
                     /translation="MTVEAGRKLGRYVLSRKLGAGGMAEVWEAFDEGLHRSVAVKVVR
                     DEIAGEAEYRERFIREARLAAGLEHPRILPIYDFGTEAGITFLVMPLLPGGSLRERIT
                     GPMHPEEAIDCLASIAAALDHAHARGILHRDVKPSNVLVDASGSLLLADFGLAKSTAV
                     SSELTATGMVVGTPAYMAPEQAIGRPVDARADQYALGVLGFELLTGRTPFQSESPFAV
                     LDKHLREAPPPPSTFVPALPSPVDAVLARALSKQPQERFATCREMVEALAGALGVSVP
                     ARPATAVRPVGALDPTWIAGSGAAPPPLATPPPSWPPPPPSNVTLPAPRTAVTMRRPA
                     PTGLPAGAIAAIAVAALVFVGGVAAAGWFFFRRPPAGGEPPAAAASPSASTVVPTLPP
                     TVIPVEPSPASTDADSSSAEDASTVTIEELAIPTAVPSLAAVAATPFPSPSASPQLAA
                     ATRRTPPSATSSAAPTPEVSTPDRASEPARPVESDPSSRLERVVPFRTGEAVPVGIVD
                     RSITIESVAVTDWPKPEELRKAEARPGATIGLTVKFSYTNRDDEDWSCTYRVTVLDAE
                     GREIGSGVQKRSLGGSEAGDTNRVSVKMRAADFPRAAKLRVRILANPD"
     gene            complement(6741..7517)
                     /locus_tag="P5164_03315"
     CDS             complement(6741..7517)
                     /locus_tag="P5164_03315"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRY42956.1"
                     /translation="MADAKAETRRLAVDLGLPEGSVHVEMDVPTGEVPMEGLLPALRT
                     TADAFVAFGAALSARDGKPVSCAKGCGACCRQVVPIAPAEARRVAALVEEMPEPRRSL
                     VKGRFAEAWKRIEEAGLMPALEGQGPRAAGGAVEVGLAVFRLGIPCPFLEDESCSIYE
                     DRPIACREYLVTSDPAHCAEPTPETIAPVPLPTRVWAAVAREEQGIPDGDPVPLVPLV
                     LAPRWAAANPEGGARRPGPELLRSVYGRFASDLSRGREKR"
     gene            complement(7544..9196)
                     /locus_tag="P5164_03320"
     CDS             complement(7544..9196)
                     /locus_tag="P5164_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRY42957.1"
                     /translation="MDEESLIPLLARSLRRNAKLPAFSDFPGPAMTYDDVAVRIARYH
                     AAFRELHVKPGTKVGLLGRNSAGWATAWLAVVTCGAVAVPILPDFREEDVHHILNHSE
                     AALLLTTDAFFERLDPEKTPGVKAVLSTKDLSPLFLRREGAAGALRKADAAARSAAPP
                     SVPDAFASAGPEDLASIVYTSGTTGFSKGVMLPHRSLLRNVLYAQRHMPLEAGDAVVS
                     FLPLAHAFGCAFEFLFPFSVGCRIAFLDKTPSPQVILKAFATEKPRLVLTVPLVLEKI
                     YARRVRPFLEKPAVRLLTRLPALRRKVDALLLRKLVDAFGGRVLEVVVGGAALNPAVE
                     EFLLRIGFPVTVGYGMTECGPLISYSGWREHRPQGVGRAVDGLELVVDSPDPARVPGE
                     VKVRGPYVMAGYYKSPEATREVLGEDGWLRTGDLGLLDTDGSLSLKGRSKTMFLGATG
                     QNVYPEEIEARLNALPLVAESLVVERDHRLHALVYPDLEAADAAHVGHDGILREMEKN
                     RQKLNAKLPPFCQVASIEVVPEELQKTPTKKIKRFLYAGRAG"
     gene            9286..9807
                     /locus_tag="P5164_03325"
     CDS             9286..9807
                     /locus_tag="P5164_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRY42958.1"
                     /translation="MSRSVDFARLDLKDALDLAILIEDEAQERYEEFAEQMEVHHTAD
                     AAKFYRFMAENEAKHGAELAARRKVFFGDAPTRMSRSMLWDVEAPEYDKARAFMSPRK
                     ALEVALESEIKAHDYFTAALPFLSDPDVKALFTELQGDEVEHKALVQAELAKLPPEPE
                     IDVEDLVDEPVGQ"
     gene            complement(9831..12521)
                     /locus_tag="P5164_03330"
     CDS             complement(9831..12521)
                     /locus_tag="P5164_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRY42959.1"
                     /translation="MTPVAGSRLGPYEVVAPIGRGGMGEVFRARDTRIGREVALKVLS
                     GTACADEERFRRFELEARAAGALSHPNVVVLHDVGTEAGTPYLVSELLHGETLADRLR
                     RGAIAPRRAVEIAIDVASALAAAYEKGIVHRDLKPENVFLTADGQTKVLDFGLAKLLG
                     DGPPGGKGGTPAPETRSDVVVGTAGYMAPEQVRGEELDGRADIFALGTCLYEMLSGHR
                     AFGAGSAVETMAAILLSEPPDLTTLRQGIPRQLDRIVGRCLEKQRGARFQSARDLAFA
                     LESLDIASLPSEPGRAGATRRLRGRGGRRRFLGPALLVAAGAAAGVLGALALRGPAAD
                     PPAALRTLTHSGVDAEPAASPDGGLVAFTSRRGGRSRIWLKQVRGEGETALTAGPGDS
                     FPRFFPDGESLLFTRTDGSAGPSLFRVPLFGGAERRLFGAAESGDVSPDGAEVAFLRL
                     LRPADRVTALLLGPASGGEARELARFDTVVASPRFSPDGRTIALSEEGSAQSGVAGSI
                     VLVGRDGRVRRLPLPLAARQPTAVAWLGSGRELLAGVFDASVPHARTVGLRVLRMPAT
                     GGAAQVVAWWPSAGRTLDVLAEGRVVFDSVATRESLYEVPIGRGGPVRWLTHGTSTDR
                     QPVYSPDGKRILFSSDRSGQLDLWVLERETGALRRVTDDPAQDWDPAFSPDGRRILWS
                     SDRSGRFEVWTSNVDGTEARRLTRGEADAENPVLTPDGGTLLWGSLERGRGGIWRARA
                     DGSGAAPLVRGQALQLPEVSPDGRWVAFREGIGTGPRAIRVVRLADGAEVASVVLAEV
                     VDAVTAGESGRPRWMPDGRLAFVGVDEQGLTGIFAQEIVAGADTTATRRRLGGFDPTL
                     AAESFAISPDGAALIVAARESLSSVVLAEPVAGAPKPRRP"
     gene            complement(12580..13251)
                     /locus_tag="P5164_03335"
     CDS             complement(12580..13251)
                     /locus_tag="P5164_03335"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HRY42960.1"
                     /translation="MAFTKSGARRLLVPLLLALLVYAFWSTLAVWPLRLFVVLLHEVS
                     HGLAAVLTGGRIVSIELSPREGGLCTTAGGWPFLISSAGYLGSALFGALFLLLGVRGR
                     PRTHRVATGVLGAALLLLTLVWVRSAFGFVYGLAAGVLLLGVARWLPEGASSFVLRLL
                     GVTSLLYAPWDITSDLILRSVPASDASALSRMTGIPALAWGVLWLAASVWIAWRAVRG
                     IGGRG"
     gene            complement(13258..15033)
                     /locus_tag="P5164_03340"
     CDS             complement(13258..15033)
                     /locus_tag="P5164_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005035371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRY42961.1"
                     /translation="MALVDPHSYADDTQPVTKHLRLHLAVDFERKRIDGRAVLELAAP
                     AGGPMDLDTKGITVSAVTASGGGEVLFDLGPEDPILGRRLRLKLPAGTTEVLLRYHTS
                     PEAIGLQWLAPEQTEGKRHPFLFSQCQAIHARTMVPCQDTARFRVTYHAEVTVPAPLS
                     AVMSAGPSGVIEGKGTRTFLFDMPQPIPTYLLALAVGELESRDLSPRARVWAEPATVE
                     KAAWEFAGVEEMIVKAEGLFGPYDWDRYDMLVLPPSFPYGGMENPRMTFLTPTLLAGD
                     RSLVDVVAHELAHSWTGNLVTNATSEHFWLNEGFTVWAERRILEAIHGEEAATLGWAI
                     GEKALADSIARFGEGSPLTKLRTHLQGIDPDDAFSSVPYEKGARLVVLLERTVGRAAW
                     DAFLADYMKRFRFQSITTEEFLAFLEEKLPGAAAKANADAWLHEPGLPANAPVFRSEK
                     AEALAALAAGWPDGVRPTSEQVGSWSASETLLYLQKLPRQLPAEECAALDAAFGLSGK
                     GNYELLVEWLTIAAGSDYEPAFARIREVLSRVGRMKYLRPLYGALAATPRTRALAREI
                     FAAASPTYHALSRRVVAGVIEKYPA"
     gene            15114..>16232
                     /locus_tag="P5164_03345"
     CDS             15114..>16232
                     /locus_tag="P5164_03345"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY42962.1"
                     /translation="MSEAKPAGLLKSFPRVFWVANVMELFERGAYYGLNSVLAVYLTA
                     DVAKGGLGFGEGSVGFLQSLIYACTYVVPILGGALADRYGYRRMLLVCFALLSTGYFA
                     AGHMSSYGAVFASLLVMAMGGGLFKPIISGTIARSTNESNSGFGFGIYYWMINLGAFL
                     APLVVSILKGFSWRYVFLASSAYVALMFLPTIFVFRDPPLPENRKTLAEVVQGAALVL
                     GDARFMLMIVVYSLFWILYFQNFGSVLWFLRDFIDPAPMNAVFAALRLPFRFDAEFVT
                     VINAGTIILLQVFVSRIVKDLKPLPTMLGGVAIGALGFLCLAFAQNVWLFIAGIAVFS
                     VGEMTAHPKYYSYVGLVAPQDKKAVYMGYAFLYGVFGSL"
BASE COUNT         2277 a         5886 c         6009 g         2060 t
ORIGIN      
        1 cgctcggcct tcgccgcgac gtcccagagc cggagctcgc cgcccagctc gtagacgacc
       61 ttcccggcgt tgtcggagct ggcccagcgg acgtcccacg tcgtcgacct cgtcagctgc
      121 tcgaggcccg agccgtcgac gccgaccgag tagaggttca gcgtgccgtc gcggtcggag
      181 acgaagaaga cccgctcccc gatccacatc ggatcgcgct ccgtcctcct cgacggggcg
      241 atcgccctct gcgtcccggc cgccaggtcg tagatccaga ggtcctgcgc ccagccccct
      301 tcgtagcgct tccaggtgcg gaagtcgcgg aagagcggcg agtagacgag gcgcttgccg
      361 tcggggctga aatccccggc tcccgccgtg tacatcggga gcttcgccgg cagcccgccc
      421 gcgaccggca cggtgtagag cgccgtctcc gtcttcgcgc tttcagcgtc acgcagggag
      481 cggaagagga ccgcctttcc atcgggcgtc cagccgtaga cctggttgtc gccgccgtgg
      541 cggggagcgc cgggtccccg cgaggggtac caggtgaggc gcttcgggac gcccccttcg
      601 gccgggacga cgtagacctg ctcgtctccc tcgtactgtc cggtgaaggc gatccacttc
      661 ccgtcgggcg agaacttcgg gaagagctcc tgccccgcgt gcgcggtgac ccgtgccgcg
      721 aggccgccgg agacgggcgc cgtccagacg tcgccgccgt agcagaaggc gacgcgctcc
      781 ccgtggacgt cggggaaccg aagcagcttc gtctgggacg cggccggcga agcgagcgcg
      841 agggccgcga agcccgcggc gaagggggcc gagaggcgcg cgaggcgccg gagcgaacgg
      901 ttcatcggat cctccggacc ggcatcctag cgcgcgccgc gccctcgccc tggggcccgc
      961 cccggcgccc gccccggtgc caggcgtgaa atcgtgtatt gttgaaacaa tacacgattt
     1021 cacgcctggc accagtctcg gggggcgtca gggcttcagg gtcttccgat cgaaggcgag
     1081 ccagcgggcg aggtcggtgg cgtaccagcg ggagaagcgg cggtcggagg ggccgccggg
     1141 gagggcagtc tcggcgacgt ccgtcgcgag gtcggtgacg aaccgccagg aagggcagat
     1201 cgtccgttcg cgtcccgcga gcgagaggag ctgcccctgg gcgacggtcg cgcggcctcc
     1261 gacgacctcg aacggcccgc ggtcgaagcc gaagaccggc gggagcttcc cgccgaagag
     1321 gaggtgccgg aggaagacgc gccgggaggc gccccacggc aggagcgcgg agggatcggc
     1381 cccggcgagc gcccggcggg cgacacggga gacgaaggcg gccttcccct ccgcgccgaa
     1441 ccactcctcc ccctcctcgc gcgcgaggag gtcgtcgaag aggcggaaga agaacgcatg
     1501 gacgagcgtc gtgtcgcgga gcgcgcgcca cgcgtcccgt ccgagcagcc ccgcgccgaa
     1561 gagctcctcg cagagggccg cgtagagcct ttcgaagagg acggccccct tcgaggcggc
     1621 gtcgtagcgg cggtcccacg cgcggagctc gcggaccgcg gggaggtcag ggaggtacgg
     1681 ctcgagcgcc gagaggagcc gccgcgcctg gccggagacg aggtccgcct gcatcgcgcc
     1741 gaggtcggcg gcggagaggc ccccgccggc ggcggccttc gcgccggcga gctcgcgaag
     1801 gcgatcggcc cggtcggagc ccatgcagag cgtgatcccg accggcttcc acgcgccgtg
     1861 gccttcgtcg ttcgcggtga cgagaacccc ttccgcggag ccggcggcgc gcgcgaggga
     1921 aggcggcgac agcgtgccgt cccagaggtc gcccgcttcc cagccgcgca gcgggaggag
     1981 gccgggggcg cggcggcgcc tcggcaggag gcccgactgc tgcgccgcga tccggccgtc
     2041 gcggcccgcg aggagccagc tggcgctgag cgcgacgcgc gcggcggcgt cggcgccccg
     2101 gtcgacgtcc ggcgccgccc agaggtcgag gatcgcctcg agcgaggcgg cgcccccctc
     2161 gcgccggccg gtccaggcgc gggcgatcca gagcccgtcc tcgacgcggg ccacgccgtc
     2221 cggggtctcg aggaccccgg cgtcggtctc gaagacgacg agctcccgcg gcggcccctt
     2281 cttcctccgg accgtctccc gcctctgccg gagcgcgagc cacctctcgc cgcggcggaa
     2341 acgcccgtcc cgcacctcct cgatcacgag gtcgatggtg tcggcgaatc cgtacgtgaa
     2401 ccccgccgcg agcgcgcgcg tccgccccat gacgaggccg gggacgccgg gcacggtgac
     2461 tccggtccgg aagtcgccgc cggggagcgt gccgacgacc tcgtaccaga tcgccggcag
     2521 gcagttcacc tcgagatggg ggtcgctcgc gtagagggcc gcgcccgagg ccgagcgccc
     2581 ccccgcgacg gcgacgttgt tgctggcgcg gagggtcggg agggccggca ggcccggcac
     2641 gagcgccgga acgtgccgga gcgagcggat cgcctcgagg agcgcgtcat ccagcccgtc
     2701 gaggtgcggg cggaagaggc gggcgagctt gccgcggtcg acgccggccc ggagggcctg
     2761 gaggatcgcg acctcggcgt cgctctgcgc ctgcgcgagg ccgacgaagg tcatgagccg
     2821 gagcgtcagg agcgtgtcgg ccggcgtcca cggctcggcg gggacgcgga ggagccgaag
     2881 ctcccagggc cggcccctcg cgaggcgcgc gttcacgccg gccgcgtagg cctccgtcag
     2941 gcttctcgcg cgaaccggaa gagacgccgc ctcttccgcg gaggccgccg cgaagaggcc
     3001 ccgccgcatg aaaaggtcga tctcgagcga ggcgggcgag tcgtcgagga gctcgcagag
     3061 cctcccctgc ccgacgatcc gggtgacgag gagctggagg gcgcggtcgt gcgcgtgggc
     3121 gaaaccccac gccgccgcga ggccgaggtc gtcgccggcc tccgcgtgca tcacgccggc
     3181 gccgtccctc cggaggacga aggggcgccc tcccgcttcg aacgtgcggg gctccgaccc
     3241 gatccgaggg ctcaccgtcg cgcgattcta cggacccgcg cgacggcgcg cggagctcag
     3301 tgcccggtgc agcccgggac ccggccgagc gagacctctt ccccgtggag agagagcttg
     3361 ccggccgagc cgaggaactc gaggaggccc tcgaaatgga accggtcgcc gtggcagttg
     3421 ccgtagagag cgtccgcgcc gaacgcttcg gccgccgccc gcttcagctc ggccaccggc
     3481 agtcggccac cccggtcggc gaggatgcgg aggacctcgt gaccgaagac gaagccgtcg
     3541 gcctcgtggg aatggtcgtg gtggtggtcg tgggcctcga tcgtcatggc gctcaccgta
     3601 gcacgcgccc ctccggcttc cgggatgacg gacgtcatcc cgcgagccgg ttccgccgcc
     3661 tcagcgcgga agcgcgggcg ggagcccccg ctccgtcagg aggtagaccg cgaacgtccc
     3721 gagccagtgg ctcccctcgt agtgctccga cgtgacgctc gggaggctcg ccttcgcatg
     3781 ggcgtccgcg gcggcccgga gcgcggggat gcggcggtcc cgcggcggga gggaggccgc
     3841 gatcccccgg agcatgaaag cccgcgagag gttcaggccg tcgaggtgga cgagcttgcc
     3901 gtccgtccgg tcggtgacga tcccgggagc gagccagccc gacgacccgt cccgcgggag
     3961 gtccgggaga agccccgaga gccacctcgc gtagcgatcg ctcggcatca cccgacgcat
     4021 caggtcggcc tccgcgaggc acggcgagag gaaatcctgc cccgaaggct cgtacccgat
     4081 cgggcaccct cggtcggagc cgtagagctc gacgatccgc cgggcgagga gccccgcctt
     4141 ctccgtctcc cctgcgccgc gggcccagtc gaggacgagg ccgaacgcga aggcggtctg
     4201 cgggtgctct ccttcccgga tcgggtaggc gagcttcggg atccaggcga agaggagatc
     4261 ggcggccttc gcctcgagcg gtgcgagcgc cctcgaccac gcccgcgcct ccgggtcgtc
     4321 ccactcgcgc agctccgcgg cgagctggag gagccacgcg aggccgtacg gccgctcgaa
     4381 gccggtccgc ccgggtgcgg cgagataggc gacctccccc gcgacgttcc cctcggtgag
     4441 gctcctcgcc agcgccgcgc gcgccggagc cgtgtacgac gcctccgggt ggagccgggc
     4501 gaggcgcgcg aggagccagt gcccgtggac cgaggagtgc cagtcgaagc agccatagaa
     4561 ggccggggtc agctcgcgcg gcggcttcgc gtcggcgtcg ccgttcatca cgtgggcgat
     4621 cttgttcggg tactcgcgat ggacgcagtc gagggcgaga cgcgcaaacc gtccggccgc
     4681 ggcctcgtcg agatcggagc cgtccgtcgc cccggcggcc ggcgcgacga gcagggcgag
     4741 cgggaggagg atcgcgcgga gtcgcacgag gcgattctag gcgccgcggg ggcacccggc
     4801 ggcgggggac cccgatccgc gcccaccctc gattcgcgcg gagggggagg cgccgaaggc
     4861 gccgcggggg cacccggcgg cgggtgcccc cgatccactt cagtccggat tggccagtat
     4921 tcgcaccctc agcttcgccg cgcgcgggaa gtcggcggcc cgcatcttca cagagacgcg
     4981 gttcgtgtcg cccgcctccg agcccccgag gcttcgcttc tgcaccccgg agccgatctc
     5041 ccgcccttcc gcgtcgagca ccgtcacccg ataggtgcag ctccagtcct cgtcgtcgcg
     5101 gttcgtgtag ctgaacttca ccgtcagtcc gatcgtcgct cccggcctgg cctccgcctt
     5161 ccggagctcc tccggcttcg gccagtcggt gacggccacc gactcgatcg tgatcgaacg
     5221 gtcgacgatc ccgaccggca ccgcctcgcc cgtgcggaac ggaacgacgc gctcgaggcg
     5281 cgaggacggg tcggactcca ccggccgggc cggctccgac gcgcggtccg gcgtcgagac
     5341 ctccggcgtg ggcgcggcgg agctcgttgc agacgggggc gttcggcgcg tcgccgccgc
     5401 gagctgcggc gaggccgacg gcgacgggaa gggcgttgcg gcgacggccg cgagcgacgg
     5461 caccgcggtc gggatcgcga gctcctcgat cgtgacggtc gacgcgtcct cggccgagga
     5521 ggagtccgcg tccgtcgaag ccggggacgg ctcgaccggg atgacggtgg gggggagggt
     5581 cggaacgacg gtcgacgcag acggtgacgc ggccgcggcc ggaggctcgc cgccggccgg
     5641 cgggcgccgg aagaagaacc agcccgcggc ggccacgccg ccgacgaaga cgagggccgc
     5701 cacggcgatc gcggcgatgg cccccgcggg gagacccgtc ggggccggac ggcgcatcgt
     5761 caccgcggtc ctcggcgccg gcagcgtgac gttcgacggc gggggcggcg gccaggacgg
     5821 cggcggcgtc gcgagcggcg gcggcgccgc gcccgatccg gcaatccagg tcgggtcgag
     5881 ggcgccgacg ggccgcacgg ccgtggccgg acgggccggg acggagacgc cgagcgcgcc
     5941 ggcgagcgcc tcgaccatct cgcggcaggt cgcgaagcgc tcctgcggct gcttcgagag
     6001 cgcccgcgcg aggacggcgt cgaccggcga cgggagcgcc gggacgaagg tggagggggg
     6061 cggcggcgcc tcccgcaggt gcttgtcgag gacggcgaac ggggactcgc tctggaacgg
     6121 agtcctgccg gtcaggagct cgaacccgag gacgccgagg gcgtactggt cggccctcgc
     6181 gtcgacgggc cggccgatcg cctgctcggg cgccatgtag gccggcgtgc cgacgaccat
     6241 ccccgtcgcc gtcagctcgg acgacaccgc cgtgctcttg gccaggccga agtcggcgag
     6301 gaggaggctg ccggaggcgt cgacgaggac gttcgagggc ttcacgtcgc ggtggaggat
     6361 gccccgcgcg tgcgcgtggt cgagcgccgc cgcgatcgac gcgaggcagt cgatcgcctc
     6421 ctcggggtgc atcgggcccg tgatccgctc ccggaggctc ccgcccggca gcagcggcat
     6481 gacgaggaag gtgatgcccg cctcggtccc gaagtcgtag atcggcagga tgcgcgggtg
     6541 ctcgagcccg gcggcgagcc gcgcttcgcg gatgaaccgc tcgcggtact ccgcctctcc
     6601 ggcgatctcg tcccggacga ccttgaccgc gacgctccgg tggagcccct cgtcgaacgc
     6661 ctcccagacc tcggccatcc cgcctgcgcc gagcttgcgc gagaggacgt agcgtccgag
     6721 cttgcgcccc gcctcgaccg tcatcgcttc tcccggcccc ggctcaggtc gctggcgaaa
     6781 cggccgtaga cgctgcggag cagctccggg cccggccggc gcgcgccccc ttccgggttc
     6841 gcggccgccc agcgcggggc caggacgagc ggcacgagcg gcaccgggtc cccgtccggg
     6901 atcccctgct cctcgcgcgc gacggcggcc cagacgcgcg tcggaagggg gaccggggcg
     6961 atcgtctccg gcgtcggctc ggcgcagtgc gcaggatcgg acgtgacgag gtactcgcgg
     7021 cacgcgatcg ggcggtcctc gtagatcgag cagctctcgt cctcgaggaa ggggcagggg
     7081 atgccgagcc ggaagacggc gagaccgacc tcgaccgccc cgccggccgc gcgcggcccc
     7141 tgcccctcga gcgccggcat cagcccggct tcctcgatcc gcttccaggc ctcggcgaag
     7201 cgccccttta cgagggaccg cctcggctcc ggcatctcct cgacgagcgc ggcgacgcgc
     7261 cgcgcctctg ccggggcgat cggcacgacc tggcggcagc aggccccgca gcccttcgcg
     7321 caggagacgg gcttcccgtc ccgcgcggag agggcggccc cgaacgcgac gaacgcgtcg
     7381 gcggtcgtcc ggagcgccgg gaggagccct tccatcggca cctcccccgt cggcacgtcc
     7441 atctcgacgt ggacgctccc ctcgggaagc cccaggtcca ccgccagccg gcgggtctcg
     7501 gccttcgcgt ctgccatgag cgcgaattag agcagacccg gggtcagccc gcccggcccg
     7561 cgtagaggaa gcgcttgatc ttcttcgtcg gggtcttctg gagctcctcg ggcaccacct
     7621 cgattgaggc gacctggcag aacggcggga gcttcgcatt cagcttctgc cggttcttct
     7681 ccatctcccg caggatcccg tcgtgcccca cgtgcgcggc gtcggccgcc tcgaggtcgg
     7741 ggtagacgag ggcgtggagg cggtgatccc gctcgacgac gagcgactcc gcaacgagcg
     7801 gaagcgcatt caggcgggcc tcgatctcct ccgggtagac gttctgtccg gtggccccga
     7861 ggaacatcgt cttcgaccgc cccttcaggg aaagggagcc gtcggtgtcg agcagaccga
     7921 ggtccccggt ccggagccag ccatcctcgc cgaggacctc tcgagtcgcc tcggggctct
     7981 tgtagtagcc ggccatgacg tacgggccgc ggaccttcac ctcccccggc acccgcgccg
     8041 ggtcgggaga gtcgacgacg agctcgaggc cgtcgacggc gcgccccacg ccctgcgggc
     8101 ggtgctcgcg ccagccggag taggagatga gcgggccgca ctcggtcatc ccgtacccga
     8161 ccgtgaccgg gaagccgatc cggaggagga actcctccac cgccgggttc agggcggcgc
     8221 cgccgacgac gacctcgagg acccggccgc cgaaggcgtc gacgagcttg cggagcagga
     8281 gggcatccac cttccggcgg agcgccggga ggcgcgtgag aagccgcacg gcgggcttct
     8341 cgaggaacgg cctcaccctg cgggcgtaga tcttctcgag gacgagcggg accgtcagga
     8401 cgagccgcgg cttctcggtg gcgaaggcct tcaggatgac ctgcggggac ggcgtcttgt
     8461 cgaggaacgc gatccggcag ccgacggaga aggggaagag gaactcgaac gcgcacccga
     8521 acgcgtgggc gagcgggagg aacgagacga cggcgtcgcc cgcctcgagc ggcatgtgcc
     8581 tctgcgcgta gaggacgttc cgcagcaggc tccggtgcgg gagcatgacg cccttggaga
     8641 agccggtcgt cccggaggtg tagacgatgg aggcgaggtc ctccggtccg gcgctcgcga
     8701 acgcgtccgg gacgctcggc ggcgcggcgg agcgcgccgc ggcgtcggcc ttccggagag
     8761 cgccggccgc cccctcgcgc cggaggaaga ggggcgagag gtccttcgtg gagagaacgg
     8821 ccttcacgcc cggcgtcttc tcggggtcga ggcgctcgaa gaacgcgtcg gtcgtcagga
     8881 gcagagccgc ctcggagtgg ttcaggatgt ggtggacgtc ctcctctcgg aagtccggca
     8941 ggatcgggac cgcgaccgcc ccgcaggtga cgaccgccag ccaggccgtc gcccagcccg
     9001 ccgagttgcg tccgagcagc ccgaccttcg tccccggctt cacgtgcagc tcgcggaacg
     9061 cggcgtggta gcgcgcgatg cggaccgcga cgtcgtcgta ggtcatcgcc ggcccgggga
     9121 agtcggagaa ggcggggagc ttcgcgttcc ggcgcaggct ccgcgcgagg agcgggatca
     9181 ggctctcctc gtccatcgac cggccatctt acgatgcgtc cgccgcgtcc ggcgggctaa
     9241 gatccgcgcg gttggagccg ccggagcggc tccgggagtg agccgatgag ccgaagcgtc
     9301 gatttcgcac gtctcgacct gaaggacgcc ctcgacctcg cgatcctgat cgaggacgag
     9361 gcgcaggagc gctacgagga gttcgccgag cagatggagg tccaccacac ggcggacgcg
     9421 gcgaagttct accgattcat ggccgagaac gaggcgaagc acggggccga gctcgccgca
     9481 cggcggaagg tcttcttcgg ggacgccccg acccggatga gccgctcgat gctctgggac
     9541 gtcgaagccc ccgagtacga caaggcgcgc gccttcatgt ccccccgcaa ggccctcgag
     9601 gtcgccctcg agtcggagat caaggcgcac gactacttca ccgccgccct cccgttcctc
     9661 agcgaccccg acgtgaaggc gctcttcacg gagctccagg gcgacgaggt ggagcacaag
     9721 gcgctcgtgc aggccgagct cgcgaagctc ccccccgagc ccgagatcga cgtcgaggac
     9781 ctcgtcgacg agcccgtcgg gcagtagggg aacccggggg tccggtttct tcaggggcgg
     9841 cgaggcttcg gggcgccggc gacgggctcg gcgaggacga cgctcgacag gctctcgcgc
     9901 gccgcgacga tcagagcagc gccgtccggc gagatcgcga acgactcggc ggcgagcgtc
     9961 gggtcgaatc ccccgagccg gcgacgggtc gccgtcgtgt cggcgcctgc gacgatctcc
    10021 tgcgcaaaga tgccggtcag cccctgctcg tcgacaccga cgaaggcgag gcggccgtcg
    10081 ggcatccacc tcggccggcc cgactccccc gccgtcaccg cgtccacgac ctcggcgagg
    10141 acgacggaag ccacctcggc gccgtccgcg aggcggacga cgcggatcgc ccgcggcccg
    10201 gtgccgatcc cttcccggaa cgcgacccag cggccgtccg gagagacctc ggggagctgg
    10261 agcgcctgcc ctctgacgag cggcgccgcc cccgacccgt ccgcgcgtgc gcgccagatg
    10321 ccgccccgcc cccgctcgag cgagccccag aggagcgtcc cgccgtcggg ggtcagaacg
    10381 gggttctcgg cgtccgcctc gcctcgcgtg agccttcgcg cctcggtccc gtcgacgttc
    10441 gaggtccaga cctcgaaacg gcccgaccgg tccgagctcc agaggattcg tcgcccgtcc
    10501 gggctgaagg ccgggtccca gtcctgggcc gggtcgtccg tcacacgccg gagcgcaccg
    10561 gtctcgcgct ccagaaccca gagatcgagc tggcccgagc gatccgagga gaagaggatc
    10621 cgcttcccgt ccggcgagta gaccggctgc cggtccgtcg acgtgccgtg cgtcagccag
    10681 cggaccggtc cgcccctccc gatcgggacc tcgtagaggc tctcgcgcgt cgcgaccgaa
    10741 tcgaagacga cccgtccctc ggcgaggacg tcgagcgtcc gcccggccga gggccaccag
    10801 gcgacgacct gcgctgcgcc gcccgtggcg ggcatccgga gcacgcggag cccgaccgtc
    10861 cgagcgtgag ggacgctcgc gtcgaagacc cccgcgagga gctcccggcc ggagccgagc
    10921 caggcgaccg ccgtcggctg gcgcgcagcg agcggcagcg ggagcctccg gacccgtccg
    10981 tcgcgtccga cgaggacgat cgaccccgcc acgccgctct gcgcgctccc ctcctccgag
    11041 agggcgatcg tccggccgtc cggcgagaac cgcgggctcg ccacgacggt gtcgaagcgg
    11101 gccagctccc gcgcctcgcc cccggaggcg gggccgagga ggagggccgt gacgcgatcg
    11161 gccggccgca gcagccgaag gaacgccacc tccgcaccgt cgggcgagac gtctcccgac
    11221 tcggcggcgc cgaagaggcg gcgctcggcg ccgccgaaga gcgggacccg gaagagggac
    11281 ggccccgcgc tcccgtccgt gcgcgtgaaa aggagcgact ctccgtcggg gaagaacctc
    11341 gggaaggagt cgcccggccc ggccgtcagg gccgtctccc cctcgccgcg gacctgcttc
    11401 agccagatcc gcgagcggcc gccgcgccgt gaggtgaacg ccacgagccc gccgtcgggg
    11461 gaggccgcag gctctgcgtc gactccggag tgggtcagcg tccggagcgc ggcgggcgga
    11521 tccgctgccg gtcccctcag cgcgagcgct ccgaggaccc ccgcggcggc cccggcggcg
    11581 acgagcagcg cggggccgag gaaccggcgg cgcccgcccc ggccccgcag gcgccgcgtc
    11641 gccccggccc gtccgggctc ggacggaagg gaggcgatgt cgagcgactc gagcgcgaag
    11701 gcgaggtccc gcgccgactg gaagcgcgcg ccccgctgct tctcgaggca ccgtccgacg
    11761 attcgatcca gctggcgcgg gatcccctgc cggagcgtgg tcaggtcggg gggctccgag
    11821 aggaggatcg cggccatcgt ctcgaccgcg gacccggcgc cgaaggcgcg gtgccccgaa
    11881 agcatctcgt agagacaggt gccgagggcg aagatgtcgg ctcgaccgtc gagctcctcg
    11941 ccgcgcacct gctcgggggc catgtagccc gccgtgccga cgacgacgtc cgagcgcgtc
    12001 tccggagcgg gggtcccgcc cttcccgccc ggcggcccgt ccccgagcag cttcgcgagg
    12061 ccgaagtcga ggaccttcgt ctggccgtcc gccgtgagga agacgttctc gggcttcagg
    12121 tcgcggtgga cgatcccctt ctcgtaggcg gccgcgagcg ccgaggcgac gtcgatcgcg
    12181 atctccaccg cccgtcgcgg ggcgatcgcc cctcggcgca ggcggtccgc gagcgtctcg
    12241 ccgtggagga gctcggagac gaggtacgga gtgccggcct ccgtcccgac gtcgtggagg
    12301 acgacgacgt tcgggtggct gagcgcgcct gccgcgcggg cctcgagctc gaaacgacgg
    12361 aagcgttcct cgtcggcgca ggcggtcccg gagaggacct tgagggcgac ctcccgcccg
    12421 atccgcgtat cgcgggctcg gaagacctcg cccatgccgc cgcggccgat cggggcgacg
    12481 acctcgtagg gcccgagccg cgagcccgcg acgggcgtca ttcctgccgg gctcccgggg
    12541 gcgcgatcac agtcctcgat gatatggcgc ggcgccggct cagcccctcc ctccgatccc
    12601 gcggacggcg cgccaggcga tccagaccga cgcggcgagc cagaggaccc cccaggcgag
    12661 cgccgggatg ccggtcatcc gggagagggc cgaggcgtcg ctcgccggca ccgagcggag
    12721 gatcaggtcc gacgtgatgt cccacggcgc gtagaggagg ctcgtgacgc cgaggaggcg
    12781 gagaacgaag ctcgaggccc cctcggggag ccagcgcgcc acgccgagga gaaggacccc
    12841 cgcggcgagg ccgtagacga agccgaacgc gctccgcacc cagacgagcg tcaggaggag
    12901 gagcgcggcg ccgagaacgc ccgtcgcgac gcggtgcgtg cgcggccggc cgcgcacgcc
    12961 gaggaggagg aagagcgccc cgaagagcgc gctcccgagg tagcccgcgg aggagatgag
    13021 gaagggccag cctcccgcgg tcgtgcagag ccccccctcg cgcggggaga gctcgatcga
    13081 gacgatccgg ccgcccgtca ggaccgccgc gaggccgtgc gagacctcgt ggaggaggac
    13141 gacgaagagc cggagcggcc agacggcgag cgtcgaccag aacgcgtaga cgaggagggc
    13201 gaggaggagc gggacgagga gccgcctcgc cccgctcttc gtgaaggcca cccgccccta
    13261 cgccgggtac ttctcgatca cccccgcgac gacccgccgc gagagcgcgt ggtaggtcgg
    13321 gctcgccgcc gcgaagatct cgcgcgcgag ggcgcgcgtc cgcggtgtcg ccgcgagcgc
    13381 gccgtagagc ggccgcaggt acttcatccg gccgacccgc gagaggacct cccggatcct
    13441 cgcgaaggcc ggctcgtagt cgctcccggc cgcgatcgtc agccactcga cgaggagctc
    13501 gtagttcccc ttccccgaga ggccgaacgc cgcgtcgagc gccgcgcact cctcggcagg
    13561 gagctgccgc gggagcttct ggaggtagag gagcgtctcg gaggcactcc agctcccgac
    13621 ctgctcgctc gtgggccgga cgccgtccgg ccagccggcc gccagcgcgg cgagcgcctc
    13681 ggccttctcc gaccggaaga cgggggcgtt cgcggggagg cccggctcgt gcagccaggc
    13741 gtcggcgttc gccttcgcgg ccgcgccggg gagcttctcc tcgaggaagg cgaggaactc
    13801 ctcggtcgtg atcgactgga agcggaagcg cttcatgtag tcggcgagga acgcgtccca
    13861 cgccgcgcgc ccgacggtcc gctcgaggag gacgacgagg cgcgccccct tctcgtacgg
    13921 caccgaggag aacgcgtcgt cggggtcgat cccctggagg tgcgtccgga gcttcgtgag
    13981 cggcgagcct tcgccgaagc gggcgatcga gtcggcgagc gccttctcgc cgatcgccca
    14041 cccgagcgtc gccgcctcct cgccatggat cgcctcgagg atccgccgct cggcccagac
    14101 cgtgaacccc tcgttcagcc agaagtgctc ggaggtcgcg ttcgtgacga ggttgcccgt
    14161 ccaggaatgg gccagctcgt gggcgacgac gtcgacgagc gagcggtcgc ccgcgaggag
    14221 cgtcggcgtc aggaacgtca tccgggggtt ctccatcccg ccgtagggga acgagggagg
    14281 caggacgagc atgtcgtacc ggtcccagtc gtacgggccg aagagcccct cggccttcac
    14341 gatcatctcc tcgacgccgg cgaactccca cgccgccttc tcgaccgtcg ccggctcggc
    14401 ccagacgcgg gcgcgcggcg agaggtcgcg cgactccagc tcgccgacgg cgagggcgag
    14461 gaggtaggtc gggatcggct gcggcatgtc gaagaggaac gtccgcgtcc ccttcccctc
    14521 gatcacgccc gacgggccgg ccgacatgac ggccgagagc ggcgccggga cggtcacctc
    14581 cgcgtggtac gtcacgcgga agcgggccgt gtcctggcag gggaccatcg tccgggcgtg
    14641 gatcgcctgg cactggctga agaggaacgg gtgcctcttc ccttcggtct gctccggcgc
    14701 gagccactgc aggccgatcg cctcgggcga ggtgtggtag cggaggagga cctccgtcgt
    14761 cccggccggg agcttcagcc ggagccgccg cccgaggatc gggtcctccg gcccgaggtc
    14821 gaagaggacc tccccaccgc ccgaggccgt caccgcagag accgtgatcc ccttcgtgtc
    14881 gaggtccatc gggccgcctg ccggcgcggc cagctcgagg acggcccgtc cgtcgatccg
    14941 cttcctctcg aagtcgaccg cgaggtgcag gcgcaggtgc ttcgtgaccg gctgggtgtc
    15001 gtcggcgtac gagtgggggt caacgagggc catggaggtc ccctttcgca tttcgctccg
    15061 cctcgcggcg ggagcggcat tttcccacct gttcgcctct atcatcccgc cccatgagcg
    15121 aagcgaagcc cgccgggctc ctcaagtcct tcccccgggt cttctgggtc gcgaacgtga
    15181 tggagctctt cgagcggggg gcctactacg ggctgaactc cgtcctcgcc gtctacctga
    15241 cggccgacgt cgccaagggc ggcctcggct tcggcgaggg ctccgtcggg ttcctccaga
    15301 gcctcatcta cgcctgcacc tacgtcgtcc cgatcctcgg aggggcgctc gccgaccgct
    15361 acggctaccg gcggatgctc ctcgtctgct tcgccctcct ctccacgggc tacttcgccg
    15421 ccggccacat gtcgagctac ggcgcggtct tcgcgtcgct cctcgtgatg gcgatggggg
    15481 gcggcctctt caagccgatc atctcgggga cgatcgcccg ctcgacgaac gagtcgaact
    15541 cgggattcgg cttcggcatc tactactgga tgatcaacct cggcgcgttc ctcgcgccgc
    15601 tcgtcgtctc gatcctgaag ggcttctcct ggcggtacgt cttcctcgcc tcgtccgcct
    15661 acgtcgcgct gatgttcctg ccgacgatct tcgtcttccg cgacccgccg ctgccggaga
    15721 accgcaagac gctcgcggag gtcgtccagg gggccgcgct cgtcctgggc gacgcgcggt
    15781 tcatgctcat gatcgtcgtc tactcgctct tctggatcct ctacttccag aacttcgggt
    15841 cggtcctctg gttcctccgc gacttcatcg acccggcgcc gatgaacgcc gtcttcgccg
    15901 cgctccggct gccgttcagg ttcgacgccg agttcgtgac ggtcatcaac gcggggacga
    15961 tcatcctgct ccaggtcttc gtgagccgga tcgtgaagga cctgaagccg ctcccgacga
    16021 tgctcggcgg cgtcgcgatc ggcgccctcg gcttcctctg cctcgccttc gcgcagaacg
    16081 tctggctctt catcgccggc atcgccgtct tctcggtcgg cgagatgacg gcccacccga
    16141 agtactacag ctacgtcggc ctcgtcgccc ctcaggacaa gaaggccgtc tacatgggct
    16201 acgcgttcct ctacggcgtc ttcgggagcc tc
//
LOCUS       DAOTEZ010000039        16214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2434_length_16214_cov_6.975555, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000039 DAOTEZ010000000
VERSION     DAOTEZ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 16214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16214
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2434_length_16214_cov_6.975555"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="P5164_03350"
     CDS             <1..939
                     /locus_tag="P5164_03350"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY42963.1"
                     /translation="FLQPTYLGRRTAVHQAAIGCRHRCSFCGVVSMFDGKTLLPAAER
                     VARDLLVLKERHGADAVQFYDHNFFDREETTLPLAEALSRVGLPYWCYARTDALARFP
                     AKTWELVKRSGLRMVFMGAEAASDEALRRMHKGARVDETLEATRRCREAGIVPELSFV
                     LGGPGEDEAEIETTLRFIRKLKAIHPACEVILYFWTPTPRRRRPGPDAAARTEGLELP
                     TTPEEWTEPEWVDFVCHRNAPWLSPRARRRIDDFATVLGCRFPTVQDVRLPRWGKAVL
                     SAAASWRWAAGAYGGPAELRALRRVLPLKRPEAEGL"
     gene            936..2081
                     /locus_tag="P5164_03355"
     CDS             936..2081
                     /locus_tag="P5164_03355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY42964.1"
                     /translation="MSAEAASPHVVLVNLALVPGLRAGELLARLPGLGRFAASLAEAG
                     ARVSVVARSHEDADLTVGGVRLVTVSDGEGPFAPLRSPARRVVARVRELGPDVVHLGG
                     FLFPVPTATLRAALPRRVPLVLQHHGEPPGSGRLGFLQRLLLRTADAVLVTSRLIVDE
                     WVRGGYLAPETHVEEVLEASTDLRPVDRAAARAATGIDGDPAILWVGRLHPRKDPVNA
                     LRAFEAALERLPRARLHLVYREAPLLTELKELCARNPALARAVRFVGEVPHEELPAWY
                     SGADLFLTSSPSEGSNWALIEATACGLPAACSDIPANRRVATPGSSFFAPGEPPQGAD
                     ALVRASHATAPRGEAIREHFERRLSWSVVAREAVGAYTRAIARRGGR"
     gene            2078..3229
                     /locus_tag="P5164_03360"
     CDS             2078..3229
                     /locus_tag="P5164_03360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY42965.1"
                     /translation="MRVALVVPGGVDRSLTERVIPALLWLIAELARRAELVVFALGHG
                     PAPGTWEVPGARVVDLGPLRLSRVPGLAFARRAHDLRTLLRAHGPFDAVHAFWATPCG
                     ATALAAAPRLPLVVSLAGGELAGIADIGYGCDLVPRERRKVRLTLARAAAVTAASRPL
                     VAEALRRGVDARLVPLGVEDAGFLPPAERPPGGPFRLLHAADLNRVKDTPTLLRAVRR
                     LGDRGLPVTLDVAGEDTLGGAVQAEAARLGLAETVVFHGRLPTSRLRPLLAAADLFLL
                     SSRHEAGPVAVVEAAACGVPTVGTAVGHVAEGSPLRSLAVPVGDDAALAEAAASLLLD
                     PGRRCAMGEAARAWARENRAAIAAERFAGLYGEVSDARAAAARRKRAAA"
     gene            complement(3180..4382)
                     /locus_tag="P5164_03365"
     CDS             complement(3180..4382)
                     /locus_tag="P5164_03365"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRY42966.1"
                     /translation="MSATGEAAPFSNEETMRRLGEPEVAAAGLAAVAALRDGSAAPLA
                     AFAADEARARTAAFAASLQGLGPRLAALPDVAVVPAALREHLAGEAARCAARGERILG
                     LLARLEELLATSGLRAVPLKGAALVLGKEVPAGLRPMADLDLLFDSEAGLRAAAARIG
                     DALGYAPLWNTRRHLVLAEREERVPFPACEHPENPLRIELHRSFRLEVLGSVLDATDA
                     LRRDPIRAGAWALPSRSALVRHLLFHAAEDFAAKGLRGVQAYDFAALARTSGPLAIPE
                     LRRGERGPVALAAHAVERLFPGTFEPGALERAARGVSPRALDRAAELPVLRHSRPARG
                     WTATALGLVDGTLRAARFLLRTLLPSLDEVRANVAPDASGARLAAAWAGVLTRRLASG
                     APRRLARR"
     gene            4451..4924
                     /locus_tag="P5164_03370"
     CDS             4451..4924
                     /locus_tag="P5164_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42967.1"
                     /translation="MEPSLPLHPAVIHFPIAAAFFAAGALALAHVRPGARAASLSAAA
                     LLLAVAVAGGLAAFVTGWRWADELAYLAGGWGPLPGPKAVEGLAQRHALLGLASVATA
                     AVALALVLRARRREAPPTLALLAALLASALVGATGHLGGTMVHNPPPPAAEAGER"
     gene            complement(4946..5275)
                     /locus_tag="P5164_03375"
     CDS             complement(4946..5275)
                     /locus_tag="P5164_03375"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRY42968.1"
                     /translation="MSRMTGVAAAVLGACLLAGPVRAEGPGRSSTWEAYCTVCHGPDG
                     KSNTEEGKKRNARDLTDPRWLASVSDSRLEGSIRRGRDKMPAFGRKLGNEQIKALVAE
                     IRALTPK"
     gene            5424..5864
                     /locus_tag="P5164_03380"
     CDS             5424..5864
                     /locus_tag="P5164_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42969.1"
                     /translation="MSARARLHRLAPLAVFAAVAALACGTRKSPTEPVDDPPSPDATF
                     SRVQAEVFTPSCALSGCHAGAAPAQGMNLGAGSAWAQIVGVPAVGSSRLRVAPGDVAG
                     SYLVSKLRGDATITGSRMPLGGPYLSSDREQLVVDWIRRGAPND"
     gene            5873..6670
                     /locus_tag="P5164_03385"
     CDS             5873..6670
                     /locus_tag="P5164_03385"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HRY42970.1"
                     /translation="MKRHAPLLALLLLAVPPAAAQEAPAWDPGYGMRFLSTHSPLVND
                     TGILETVFTHRFNQPVNEAGGNDLFGLDNGANIGIGLSWVPVRRVAAEVYRASSGGDY
                     ELAAKVAVLEPTAERPLGIAVRAGASWLTKYDIDRKAGAFGQLLVAWTLADRVTLAAA
                     PSYASNTPLFEDVFNVPLALQVRLGRGFLATGEYVFRNRDLDGSKGQWSFGLEKSVYR
                     HRFLLWIGNSAATTVDQTLAGDYAGGVMESNLRLGFNISRQFRIGGN"
     gene            6750..7052
                     /locus_tag="P5164_03390"
     CDS             6750..7052
                     /locus_tag="P5164_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42971.1"
                     /translation="MLRRLLLALCAVFLMQETSLGSLFVGAECFESCPDDTTPGHCPP
                     TCATCGCGSHLNPVAPRPVRVTSPASSELRTACEAALPPADSHLPDILHVPRPLLA"
     gene            7250..8539
                     /locus_tag="P5164_03395"
     CDS             7250..8539
                     /locus_tag="P5164_03395"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRY42972.1"
                     /translation="MSKVDPAVFIAVAFLGTAPLLAEGPRPPASPGASVAGPLTRADA
                     RRNALEASPGLQALGAQVRAAEGASRQARALSNPELRFEAEDLGGDPALVAAQRTLSI
                     EQGIEWFGKRSARIAAAERSRDVAALDLARGRRDLLAEVDRKFAALLGAQERDAIAAQ
                     NVETAREVTRAVASLVDAGEASPVEAARAESEEALAVIDRASARRDLELARRELARLW
                     GEDSPSFPEAAGALDASVELPDRDASLAALARLPDLARWGAEAERQASLVNAARRQVL
                     PDLTLSVGARSTSGRDGRTWVAGLAFPLPVFTQFAGARAEATARQQQAAFERRAEEVR
                     VRSEFLGAYATLERARDEARALRDEVLPRAEKVYAALGEGYRRGKFRLLDLLEARRTL
                     AQTRLRTLDALVRLATADADLKRLVPDPASDTLGADR"
     gene            8536..9882
                     /locus_tag="P5164_03400"
     CDS             8536..9882
                     /locus_tag="P5164_03400"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY42973.1"
                     /translation="MNPFFRSLSARRLAPASVLLVLAAAGCGRAVAPGGAADHPPHAE
                     ASARPETAAEASDLDRSVAELKSERCEHEVPTYTCDECRYEAGVAKAGPELFDPGHGG
                     TLATMTAGARPIAGGKGSTGEIRLNQERAVHLSPLATGVVRSILVDVGARVRAGQVLF
                     EVESGEYRQAKADLLRAEAALKLSEATAARERDLHEKGVCPMKDVLEAEAALEQARAD
                     RAAVVGRLLAYGLGRREVDALRDGRGDPQSGLMPVRAPFAGTVLERSLSLGEQAQPGS
                     RLLLLADTSRVWVMTTVYEREVPAVLRAQQQAPVRAAVTVSAYPDAVFPGRVEQVSGT
                     LDEATRTAGVRVVVENPAGLLRAGMFARVELLLPEAGGDVALPEEAVLEDEGRSFVFV
                     RMEGPYFVRRPVRTGRKRDGWVEVTGIESGAEVVTKGAFLLKSDVLRSKMGAGCAD"
     gene            9892..12972
                     /locus_tag="P5164_03405"
     CDS             9892..12972
                     /locus_tag="P5164_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HRY42974.1"
                     /translation="MTTFFQWVVSRRWAVLFVGALFLAAGLVSWLRLPIDAFPDVTNI
                     QVMVLSTAPGLSASDVEQRVTYPIEQQMGGLPKVTLVRSLSRAGLSQVVIVFQDGVDP
                     YFARQLVFERLQGARELLPPGIEPELAPLSTGLGEIFQYTLEGEGFSAMEKRTVQSWL
                     IAPRLRTVPGVTEVNSFGGEVKQYQVVVRPERLVKYGLTLREVTEALERSNANAAAGL
                     IVKGWEQTYIRGVGLFASARDIENVVLKAKDGTPVTVRDLADVEVAAQPRQGAVTRDG
                     RGETVAGMAIMLRGENSRTVVSAVKEAVARIQERLPQGMRIDVFYDRTALIEACIDTV
                     KDALLAGGLLVVLVLFLFLAELRTALIVVLSLPITFLLTFIVMGAAGLSSNLMSLGGL
                     AFSVGMVVDATIVVAENVRRHLAEHGPRGDRGKVVVEAMAEVARPVGFSVLIIGLVLL
                     PLLTLQGLEGKMFAPLALTLLFSLLTSIVVALVLVPPLSHLLLTQDPEKEFGFVHRFR
                     EGYERLLQSAMARPRTTYGIAVAVLAASAALIPRMGTDFMPPLDEGSIAVNVVRLPNA
                     SLAGSVEVAGMIEKRLLKFPEVATVVSKSGRAEISEDPMGPEQTDVFVMLRPKKDWKT
                     GRTKAKLVEAMSADLAAIPGLRAAFSQPIALRVNELISGVKSDLAIKLFGPDLAVLKG
                     IADRAAAATAGVSGARDVKVEQVSGMEEVEVRLDREAMARYELGVGDVNELLEAAFAG
                     RVVTSFVEGERRFAVMVKFPDEARRDIPAIEQLLVPSPAGPRVPLGQIASVRRVEGPM
                     QISRENGMRRVVVEANVRGRDLGGFVAEVRGALAPVLKDLPTGYFVELGGQFENQRRA
                     MRQLAVVVPMVLLLILFLLYSALGSARDAALVLLNLPFALVGGVVAAVAFRMTLSVSA
                     AVAFIVLLGIAVQNGVVLVAFFTQLRARGREAGAAVAEGCRLRFKPLVMTALTSFIGH
                     LPMVWASGSGADIQKPLAVVVMGGIVTSTLLTLLVLPVLWHALEKRTGAAPAAA"
     gene            complement(13003..14688)
                     /locus_tag="P5164_03410"
     CDS             complement(13003..14688)
                     /locus_tag="P5164_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRY42975.1"
                     /translation="MTTGRLLLALLLLAPLRLGAAEPFDVVESTIPAMQAAMKEGRVT
                     SRDLVLLSLARIATWEGRLHAAITVNPRALAEADALDRERKAGRVRGPLHGIPVAVKD
                     NVQTADLPTTGGALAFDGWVPPADATLVKNLRAAGAVILAKTQLTELANWVAASPQPM
                     PNNYNGLNGWGFNPYDARRDPREATFDGRPALNTGGSSSGAGTAASFWAASVGTETSG
                     SILSPSNQSMLVGIKPTVGRISRTGVIPITADQDTPGPMARSVTDAAILLGVLEGTAP
                     DPNDPATSACAPPPGNDYTRFLKKDGLKGARIGIPRAFYYDRVTPPGAEKPRGGLSPE
                     QAKVMAEAIEALKKEGAVIVDPADVPSVVDPDPSRNYLRWGVCTDRDEMKEKECSIVL
                     AYGMERDFNAWLASLGERAPVKSLRALREWNVAHEKAGAIRYGQSILDFSDSLDLAAD
                     RARYEADRAKDLLLTATHGLDAVMAAERLDALLFPGPSGASISARPGYPTVIVPFGTV
                     PNAPKPPFPDGFEAKPAPYGVSFAGRACSEPTLLRLAYAFEQATRRRVPPPLP"
     gene            14953..>16214
                     /locus_tag="P5164_03415"
     CDS             14953..>16214
                     /locus_tag="P5164_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012382164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase"
                     /protein_id="HRY42976.1"
                     /translation="MSHPRAGQPAEGRDLVHVPRLLAAYYTERPDPAERAQRVAFGTS
                     GHRGSSLRGSFNEAHILATTQAICEVRAAQGATGPLFLGQDTHALSEPAFRTALEVLA
                     ANGVTVRVDAADGFTPTPVVSHAILAWNRDHDGPRADGIVISPSHNPPEDGGFKYNPP
                     HGGPADTDATKAIESRANALLAAGLEGVKRVPFPRARKAATTVAHDYLTSYVDDLASV
                     VDLEAIRAAGVRIGVDPLGGASVDFWQRIAERWRLDLTVVNPRVDPAFAFMTLDWDGR
                     IRMDCSSPSAMASLIAIRDRYQVAVGNDADVDRHGIVTPSGLMNPNHYLAAAIRYLFG
                     ARDGWRKDAGVGKTLVSSSLIDRVAEGLGRKLVEVPVGFKWFVPGLLSGDLGFGGEES
                     AGASFLRRDGTTWTTDKDGMILGLLAAE"
BASE COUNT         1894 a         6254 c         5893 g         2173 t
ORIGIN      
        1 ttcctccagc cgacgtatct cggacggagg accgccgtcc accaggccgc gatcggctgc
       61 cggcaccgct gctcgttctg cggcgtcgtg tcgatgttcg acgggaagac gctgctgccg
      121 gcggccgagc gggtcgcgcg cgacctcctc gtcctgaagg agcgccacgg cgccgacgcc
      181 gtccagttct acgaccacaa cttcttcgac cgcgaggaga cgacgctccc gctcgccgag
      241 gcgctctccc gcgtcggcct cccctactgg tgctacgcga ggaccgacgc gctcgcgcgg
      301 ttcccggcga agacgtggga gctcgtgaag cgctccgggc tacggatggt cttcatgggc
      361 gcggaggcgg cgagcgacga ggcgctccga cggatgcaca agggcgcccg cgtcgacgag
      421 acgctcgagg cgacgcggcg ctgccgcgag gcgggaatcg tcccggagct ctcgttcgtc
      481 ctcggcgggc ccggcgagga cgaggccgag atcgagacga cgctccgctt catccggaag
      541 ctgaaggcga tccaccccgc ctgcgaggtc atcctctact tctggacccc gacgccgcgg
      601 cgccgccgac cgggcccgga cgcggccgct cggacggagg gcctcgagct gccgacgacg
      661 cccgaggagt ggacggagcc ggagtgggtc gacttcgtct gccaccggaa cgccccgtgg
      721 ctctccccgc gcgcgcgccg gcggatcgac gacttcgcga cggtcctcgg ctgccggttc
      781 ccgacggtgc aggacgtgcg gctcccgcgc tgggggaagg cggtcctctc cgccgcggcc
      841 tcctggcgct gggcggccgg cgcctacggc ggccccgccg agctgcgcgc cctccggcgc
      901 gtgctcccgc tgaagcgccc cgaggcggag gggctgtgag cgcggaggcg gcgtcgccgc
      961 acgtcgtcct cgtcaacctc gcgctcgtcc cgggccttcg cgccggcgag ctcctcgccc
     1021 gcctgccggg cctcggccgc ttcgcggcct cgctcgcgga ggcgggggcc cgcgtcagcg
     1081 tcgtcgcgcg ctcgcacgag gacgccgacc tcaccgtcgg cggcgtgcgg ctcgtgacgg
     1141 tgagcgacgg cgaggggccg ttcgcgccgc tgcgcagccc cgcgcgtcgc gtcgtcgcgc
     1201 gggtccggga gctcggtccc gacgtcgtcc acctcggcgg cttcctcttc cccgtcccga
     1261 ccgcgacgct ccgggccgcc cttccgcgcc gggtgccgct cgtcctccag catcacgggg
     1321 agccgccggg cagcgggcgg ctcggcttcc tccagcgcct cctgctccgc accgccgacg
     1381 cggtcctcgt cacgagccgc ctgatcgtcg acgagtgggt ccggggcggg tacctcgccc
     1441 cggagacgca cgtcgaggag gtgctcgagg cgtcgaccga cctgcggccc gtcgaccggg
     1501 ccgcggcgcg cgcggcgacg gggatcgacg gcgatccggc gatcctctgg gtgggccgcc
     1561 tccacccgcg caaggacccc gtcaacgccc tccgcgcgtt cgaagccgcg ctcgagcggc
     1621 tgccgcgggc gcgcctccac ctcgtctacc gggaggcgcc gctcctcacc gagctgaagg
     1681 agctctgcgc gcgaaacccg gcgctcgcgc gcgccgtccg cttcgtcgga gaggtcccgc
     1741 acgaggagct tcccgcctgg tactcgggcg cggacctctt cctgacctcg agcccgtccg
     1801 aaggctcgaa ctgggcgctc atcgaggcga cggcctgcgg ccttccggcg gcctgctccg
     1861 acatcccggc gaaccgccgc gtggccaccc cgggctcctc gttcttcgcg ccgggcgagc
     1921 cgccccaggg agcggacgcc ctcgtgaggg cctcccacgc gacggcgccg cgcggggagg
     1981 cgattcggga gcacttcgag cggcgactct cgtggagcgt cgtcgcgcgg gaagcggtcg
     2041 gcgcctacac gcgcgcgatc gcccggcggg ggggccggtg agggtcgccc tcgtcgtccc
     2101 gggcggcgtc gaccgctcgc tcaccgagcg cgtcatcccc gcgctcctct ggctgatcgc
     2161 ggagctcgca cgccgcgcgg agctcgtcgt cttcgctctc gggcacggcc cggcgcccgg
     2221 cacgtgggag gtcccggggg cgcgcgtcgt cgacctcggg ccgctgcggc tctcgcgcgt
     2281 ccccggcctc gcgttcgcgc ggcgcgcgca cgacctccgc acgctccttc gcgcgcacgg
     2341 gccgttcgac gccgtccacg cgttctgggc gactccgtgc ggcgcgacgg ctctcgccgc
     2401 ggcgccccgc ctgccgctcg tcgtctccct cgccggcggg gagctcgcgg ggatcgccga
     2461 catcggctac ggctgcgacc tcgtcccgcg cgaacggcgg aaggtccgcc tcacgctcgc
     2521 ccgcgccgcg gccgtgacgg ccgcgagccg cccgctcgtc gccgaggcgc tccggcgcgg
     2581 cgtcgacgcg cgcctcgtcc cgctcggggt cgaggacgcg ggcttcctcc cgccggccga
     2641 gcgcccgccg ggcggcccgt tccgcctcct ccacgccgcc gacctcaacc gcgtcaagga
     2701 cacgccgacg ctcctccgcg ccgtgaggcg cctgggcgac cgcggcctcc cggtgacgct
     2761 cgacgtcgcg ggcgaggaca cgctcggcgg cgccgtccag gccgaggcgg cgcgcctcgg
     2821 cctcgcggag accgtcgtgt tccacggccg cctcccgacg tcccgtctcc gcccgctcct
     2881 cgccgccgcg gacctcttcc tcctctcctc gaggcacgag gccgggcccg tcgcggtcgt
     2941 ggaagccgcc gcctgcggcg tgccgacggt cgggacggcc gtcgggcacg tcgccgaggg
     3001 gagtccgctg cgctcgctcg ccgtcccggt cggcgacgac gcggcgctcg ccgaagccgc
     3061 ggcgtccctc cttctcgacc ccgggcggcg ctgcgcgatg ggcgaagccg cgcgcgcctg
     3121 ggcccgggag aaccgcgcgg cgatcgccgc ggagcgcttc gccgggctct acggcgaagt
     3181 cagcgacgcg cgagccgccg cggcgcgccg gaagcgagcc gccgcgtgag gacgcccgcc
     3241 cacgccgccg cgagccgtgc gcccgaagcg tccggcgcga cgttcgcccg cacctcgtcg
     3301 agcgacggga ggagcgtccg gaggaggaag cgcgcggcgc ggagcgtccc gtcgacgagg
     3361 ccgagcgccg tcgccgtcca gccgcgcgcg ggccgggagt ggcgaaggac cgggagctcc
     3421 gccgcacggt cgagggcccg cggcgacacg ccccgcgcgg cccgctcgag cgcgcccggc
     3481 tcgaacgtcc ccgggaagag ccgctcgacg gcgtgcgcgg cgagcgcgac gggcccccgc
     3541 tcgccgcgcc gaagctcggg gatcgcgagg gggccgctcg tccgcgcgag ggcggcgaag
     3601 tcgtacgcct gcacgccgcg gagccccttc gccgcaaagt cctccgccgc gtggaagagg
     3661 aggtggcgga cgagcgccga ccgcgacggc agcgcccagg ctcccgcgcg gatcgggtcc
     3721 cgccgcagcg cgtccgtcgc gtcgaggacg ctcccgagga cctccagccg gaacgagcgg
     3781 tggagctcga tgcgaagcgg gttctcgggg tgctcgcagg ccgggaacgg aacgcgctcc
     3841 tcccgctcgg cgaggacgag gtgccggcgg gtgttccaga gcggcgcgta cccgagcgca
     3901 tcgccgatcc gagccgccgc cgcgcgcagg ccggcctccg agtcgaagag gagatcgagg
     3961 tcggccatcg gccggagccc cgccgggacc tccttcccga ggacgagcgc cgctcccttc
     4021 agcgggaccg cccgcaggcc cgacgtcgcg aggagctcct cgaggcgcgc gaggaggccg
     4081 aggatccgct ccccgcgcgc ggcacagcgc gccgcctccc cggcgaggtg ctcgcggagc
     4141 gcggcgggga cgaccgcgac gtcggggagc gccgcgaggc gcggtccgag cccctggagg
     4201 gacgccgcga aagccgccgt ccgcgcccgc gcctcgtccg cggcgaaggc cgcgagcggc
     4261 gcggcggagc cgtccctgag cgcggccacg gccgcgaggc ccgccgccgc gacctcgggc
     4321 tccccgagcc gccgcatcgt ctcctcgttc gagaagggcg cggcctcgcc cgtcgcgctc
     4381 accgcgcccc tccggctccg gccggggcgc ccgacccgcg ttccgcgatg atcacgaagg
     4441 gaggacctcg atggaaccct cgctcccgct tcaccccgcc gtcatccact tcccgatcgc
     4501 cgcggcgttc ttcgccgccg gggcgctcgc gctcgcgcac gtccgcccgg gggcgcgcgc
     4561 cgcgtcgctc tcggccgcgg cgctcctcct cgccgtcgcc gtcgcggggg gcctcgcggc
     4621 gttcgtcacc ggctggcgct gggccgacga gctcgcctac ctcgccggag gctggggtcc
     4681 gctccccggg ccgaaggcgg tcgaagggct cgcgcagcgg cacgcccttc tcggcctcgc
     4741 ctccgtcgcg acggcggcgg tcgcgctcgc gctcgtcctc cgcgcccgcc gtcgcgaggc
     4801 gccgccgacc ctcgccctcc tcgcggcgct tctcgcgagc gcgctcgtcg gggcgacggg
     4861 gcacctcggg gggacgatgg tccacaaccc gcctcccccc gccgccgaag ccggcgagcg
     4921 ctgacatgac gctccgggcc ctcccctact tcggggtgag ggcccggatc tcggcgacga
     4981 gagccttgat ctgctcgttg ccgagctttc ggccgaaggc gggcatcttg tcgcggccgc
     5041 gacggatcga cccctcgaga cgcgagtcgg agaccgaggc gagccaccgc ggatcggtca
     5101 ggtcccgcgc gttccgcttc ttcccctctt ccgtgttcga cttcccgtcc ggcccgtggc
     5161 agacggtgca gtaggcctcc caggtcgacg agcggcccgg cccttcggcc cgcaccgggc
     5221 ccgcgaggag gcaggcaccg agcacggcgg cggcaactcc ggtcattctc gacattcgcg
     5281 ttctctccct tcgcgaggag tgtcgcgcgt cgcgccccgc tctccgcatg cgcgcagcgg
     5341 gaatcccgcg acgtcgtgat ccgggtcacg ggcggcgcgc gcgctccctc ccaagaatcg
     5401 ggccacccga aaggagacgc gtcatgtccg ctcgagcccg actgcatcgg ctcgctccgc
     5461 tcgccgtctt cgcggccgtc gcggctctcg cgtgcggcac gcgcaagagc ccgacggagc
     5521 cggtcgacga cccgcccagc ccggacgcga cgttctcgcg cgttcaggcc gaggtcttca
     5581 cgccctcctg cgccctctcc ggctgccacg ccggcgcggc ccccgcgcag gggatgaacc
     5641 tgggcgcggg gagcgcctgg gcccagatcg tcggcgtccc cgccgtcggc tcctcgcgcc
     5701 tgcgcgtcgc accgggcgac gtcgcggggt cctacctcgt ctcgaagctc cggggcgacg
     5761 ccacgatcac cgggagccgg atgccgctcg gcggccccta cctctcctcc gacagggagc
     5821 agctcgtcgt cgactggatc cggcgcggcg ctccgaacga ctgaggtccc cgatgaagag
     5881 acacgcgccc ctcctcgccc tcctcctcct cgccgtcccg ccggccgccg cgcaggaagc
     5941 tcccgcctgg gacccgggct acgggatgcg cttcctctcg acgcactccc cgctcgtgaa
     6001 cgacacggga atcctcgaga cggtcttcac ccaccgcttc aaccagcccg tgaacgaggc
     6061 cggcggcaac gacctcttcg gcctcgacaa cggcgcgaac atcggcatcg gcctctcctg
     6121 ggtcccggtc cgtcgcgtcg cggccgaggt ctaccgcgcc tcctccggcg gcgactacga
     6181 gctcgccgcg aaggtggccg tcctcgagcc gacggcggag aggcccctcg gcatcgccgt
     6241 ccgcgccggc gcgagctggc tgacgaagta cgacatcgac cggaaggccg gcgcgttcgg
     6301 ccagctcctc gtggcgtgga ccctcgcgga ccgcgtcacg ctcgccgcgg ccccctcgta
     6361 cgcctcgaac acgcccctct tcgaggacgt cttcaacgtc ccgctcgcgc tgcaggtccg
     6421 gctcgggagg ggcttcctcg cgacgggcga gtacgtcttc cgcaaccgcg acctcgacgg
     6481 ctcgaagggg cagtggtcgt tcggcctgga gaagtcggtc taccgccacc gcttcctcct
     6541 ctggatcggg aactccgccg cgacgacggt cgaccagacg ctggcgggcg actacgcggg
     6601 gggggtgatg gagtcgaacc tccgcctcgg cttcaacatc tcgaggcagt tccggatcgg
     6661 ggggaactga ggcgcggcgt ccccccgcgc ggcggcgcct ccgcggtcgg ccgccgggcc
     6721 ggcaggtacg acgtgctatt tttccggtgg tgctgcggcg gctcctcctc gcgctctgcg
     6781 cggtcttcct gatgcaggag acgagcctcg gctccctctt cgtgggggcc gagtgcttcg
     6841 agtcgtgccc cgacgacacc acgccgggcc attgcccgcc gacctgcgcg acgtgcggct
     6901 gcggttccca cctcaacccc gtggcgccgc ggcccgtccg ggtcacctct cccgcctcga
     6961 gcgagcttcg cacggcctgc gaggcggcgc tccccccggc ggactcccac ctccccgaca
     7021 tcctgcacgt cccgcgaccc ctcctcgcat agacgcggtc gccggagggc ggctcgagac
     7081 gcggaccgac ggtcgcgcgt ccggacgagc ccgtcccgcg cgaccgcgga cgcccccggc
     7141 cgcccctcgg ggcggctgac ccgcgtcgcg ccggcggtcg gacacccgcc gcgctcagcg
     7201 ccctcggccg gttcgacggc cggccggcgc ctctcggagg gtccacggaa tgtcgaaggt
     7261 cgatcccgcc gtcttcatcg cggtcgcgtt cctgggaacg gcgccgcttc tcgcggaggg
     7321 tccgcgcccc ccggcctcac ccggcgcgtc cgtggcgggc ccgctcacgc gcgcggacgc
     7381 ccgccggaac gcgctcgagg cgagccccgg gctgcaggcg ctcggggcgc aggtccgcgc
     7441 cgcggagggg gcctcgcgcc aggcccgcgc gctctcgaac cctgagctcc gcttcgaggc
     7501 ggaggacctc ggcggcgacc cggcgctggt cgccgcgcag aggactctct cgatcgagca
     7561 ggggatcgag tggttcggca agcgctcggc ccggatcgcg gcggccgagc ggagccgcga
     7621 cgtcgcggcc ctcgacctcg cccgcggccg gcgggacctc ctggcggagg tcgaccggaa
     7681 gttcgcggcc ctcctcggcg cgcaggagcg cgacgcgatc gccgcgcaga acgtcgagac
     7741 cgcgcgcgag gtcacgcgcg ccgtcgcgag cctcgtcgac gccggcgagg cgtcgcccgt
     7801 cgaggcggcc cgggcggaga gcgaggaggc gctcgcggtc atcgaccgcg cgagcgcgag
     7861 gcgcgacctc gagctcgcgc ggcgggagct cgcccggctc tggggcgagg attcgccgtc
     7921 cttccccgag gctgcgggcg cgctcgacgc ctcggtcgag ctccccgacc gcgacgcctc
     7981 gctcgccgcc ctggcccgcc ttcccgacct tgcccgctgg ggcgcggagg ccgaacgcca
     8041 ggcctcgctc gtcaacgcgg cgagaaggca ggtccttccc gatctcaccc tcagcgtcgg
     8101 ggcccgctcc acctccggcc gcgacggccg cacctgggtc gcggggctcg cgttcccgct
     8161 gcccgtcttc acgcagttcg ccggcgcccg cgccgaggcg accgcccggc agcagcaggc
     8221 cgccttcgag cgccgcgcgg aggaggtgcg cgttcggagc gagttcctcg gagcgtacgc
     8281 gacgctcgag agggcgcggg acgaggcccg cgccctccgg gacgaggtcc ttccccgggc
     8341 ggagaaggtc tacgcggccc tcggcgaggg gtaccggcgc ggcaagttcc gcctgctcga
     8401 cctcctcgag gcccgtcgca ccctcgccca gacgcgcctt cgcaccctcg acgcgctcgt
     8461 ccggctcgcg accgccgacg ccgacctgaa gcgcctcgtt cccgacccgg cgagcgacac
     8521 cctcggagca gaccgatgaa cccgttcttc agaagcctct cggcccggcg cctcgccccg
     8581 gcctccgttc tcctcgtcct cgcggccgcc ggctgcggcc gcgccgtcgc acccggcgga
     8641 gccgcggatc acccgccgca cgccgaggcg tcggccaggc cggagacggc ggccgaggcc
     8701 tccgacctcg accggagcgt ggccgagctg aagtcggaac gctgcgagca cgaggtcccc
     8761 acgtacacct gcgacgagtg ccggtacgag gccggcgtcg cgaaggccgg cccggagctc
     8821 ttcgacccgg ggcacggcgg gaccctggcc acgatgacgg cgggcgcgag gcccatcgcg
     8881 ggcgggaagg gctcgacggg ggagatccgg ctcaaccagg agcgcgccgt ccacctgagc
     8941 ccgctcgcga ccggcgtcgt ccggtcgatc ctcgtcgacg tcggcgcccg cgtccgcgcc
     9001 gggcaggtcc tcttcgaggt cgagtcgggc gagtaccggc aggcgaaagc ggacctcctc
     9061 cgcgccgagg cggccctgaa gctctccgag gccacggccg cgcgcgagcg cgatctccac
     9121 gagaagggcg tctgcccgat gaaggacgtc ctcgaggccg aggccgccct cgagcaggcc
     9181 cgggccgacc gcgccgccgt cgtcggccgg ctgctcgcct acggcctcgg ccgccgggag
     9241 gtcgacgccc tgcgcgacgg ccgcggagac ccgcagtcgg gcctgatgcc ggtccgcgcg
     9301 cccttcgccg gcacggtcct cgagcgctcg ctctcgctcg gagagcaggc gcagccgggg
     9361 agccggctcc tgctcctcgc cgacacctcg cgggtctggg tcatgaccac cgtctacgag
     9421 cgggaggtcc ccgccgtcct ccgcgcgcag cagcaggcgc ccgtgcgcgc ggcggtcacg
     9481 gtctccgcct atcccgacgc ggtcttcccg ggccgcgtag agcaggtctc gggaacgctc
     9541 gacgaggcga cgcgcacggc cggcgtccgc gtcgtcgtgg agaaccccgc cggcctcctg
     9601 cgcgccggga tgttcgcgag ggtcgagctg ctccttccgg aggcgggcgg ggacgtcgcc
     9661 ctgcccgagg aggcggtcct cgaggacgag ggccggtctt tcgtcttcgt ccggatggag
     9721 ggcccctact tcgtccgcag gcccgtccgg accgggcgga agcgggacgg ctgggtcgag
     9781 gtgacaggga tcgagagcgg cgccgaggtc gtcacgaagg gcgcgttcct cctcaagtcg
     9841 gacgtcctgc gctcgaagat gggcgccggc tgcgcggact gagagggcgc cgtgacgacg
     9901 ttcttccagt gggtcgtgag ccgccggtgg gcggtcctct tcgtcggcgc cctcttcctg
     9961 gccgccggtc tcgtttcgtg gctgaggctg ccgatcgacg cgttccccga cgtgacgaac
    10021 atccaggtga tggtgctgag cacggcgccg ggcctctcgg cctcggacgt cgagcagcgg
    10081 gtgacctacc cgatcgagca gcagatgggc ggcctgccga aggtgacgct cgtccgttcg
    10141 ctctcccgcg cgggcctgtc gcaggtcgtc atcgtgttcc aggacggcgt cgacccgtac
    10201 ttcgcccgcc agctcgtctt cgagcgcctt cagggcgcgc gggagctcct cccgcccggc
    10261 atcgagcccg agctcgcgcc gctctcgacg ggcctcggcg agatcttcca gtacaccctc
    10321 gagggtgagg gcttctcggc gatggagaag cggacggtcc agagctggct gatcgcgccg
    10381 cgcctgagga ccgtcccggg cgtcaccgag gtgaacagct tcggcggcga ggtgaagcag
    10441 taccaggtcg tcgtccggcc cgagcgcctc gtcaagtacg gcctgacgct gcgcgaggtg
    10501 accgaggcgc tcgagaggag caacgccaac gcggcggccg gcctcatcgt caagggctgg
    10561 gagcagacct acatccgcgg cgtcgggctc ttcgcgagcg cccgggacat cgaaaacgtc
    10621 gtcctcaagg cgaaggacgg cacgccggtc accgtccgcg acctcgccga cgtcgaggtc
    10681 gccgcgcagc cccgccaggg ggccgtcacg cgcgacggca ggggcgagac ggtcgccggc
    10741 atggcgatca tgctgcgcgg agagaactcc cggacggtcg tctcggccgt gaaggaggcc
    10801 gtggcccgga tccaggagcg gctcccgcag gggatgcgga tcgacgtctt ctacgaccgc
    10861 acggccctga tcgaggcgtg catcgacacc gtgaaggacg cgctcctcgc agggggcctc
    10921 ctcgtcgtcc tcgtcctctt cctcttcctc gcagagctgc ggacagctct gatcgtcgtc
    10981 ctctccctgc cgatcacgtt cctcctcacc ttcatcgtca tgggggcggc cggcctctcc
    11041 tcgaacctga tgagcctcgg agggctcgcg ttctccgtcg gcatggtggt cgacgcgacg
    11101 atcgtcgtgg ccgagaacgt ccgccgccac ctcgccgagc acgggccccg cggcgaccgc
    11161 gggaaggtcg tcgtggaggc gatggccgag gtcgcgaggc cggtcggctt ctcggtgctg
    11221 atcatcggcc tcgtcctcct ccccctcctc acgctccagg ggctcgaggg gaagatgttc
    11281 gcgccgctcg cgctcacgct cctcttctcg ctcctcacct cgatcgtcgt ggcgctcgtc
    11341 ctggtgccgc cgctctcgca cctcctcctc acgcaggacc cggagaagga gttcggcttc
    11401 gtgcaccgct tccgcgaggg ctacgagcgg ctcctgcaat cggcgatggc gaggccgcgc
    11461 acgacgtacg ggatcgccgt cgccgtcctc gcggcctcgg cagccctgat cccgcggatg
    11521 gggaccgact tcatgccgcc gctcgacgag ggctcgatcg ccgtcaacgt cgtccggctc
    11581 ccgaacgcgt cgctcgccgg gtccgtcgag gtcgcgggga tgatcgagaa gaggctcctg
    11641 aagttcccgg aggtcgcgac cgtcgtctcc aagtcgggcc gggccgagat ctcggaggac
    11701 ccgatgggcc ccgagcagac cgacgtcttc gtcatgctca ggccgaagaa ggattggaag
    11761 accgggcgga cgaaggcgaa gctcgtcgag gcgatgagcg ccgacctggc ggcgatcccg
    11821 ggccttcgcg cggcgttctc gcagccgatc gcgctccggg tcaacgagct gatctccgga
    11881 gtgaagagcg acctcgcgat caagctcttc gggcccgacc tggccgtcct caaggggatc
    11941 gccgacaggg ccgcggcggc gacggcgggc gtgtcgggcg cccgcgacgt caaggtggag
    12001 caggtctcgg ggatggagga ggtcgaggtg cggctcgacc gggaggcgat ggcgcgctac
    12061 gagctcggcg tcggggacgt caacgagctc ctcgaggccg ccttcgccgg ccgggtcgtg
    12121 acgtcgttcg tcgagggcga gcggcgcttc gcggtgatgg tgaagtttcc ggacgaggcc
    12181 cgccgggaca tcccggccat cgagcagctc ctcgtcccga gcccggccgg cccgcgggtc
    12241 cccctcgggc agatcgcctc ggtgcggcgc gtcgagggcc cgatgcagat cagccgcgag
    12301 aacggcatgc gccgcgtcgt cgtggaggcg aacgtgcggg ggcgcgacct cggcggcttc
    12361 gtcgcggagg tgcggggcgc cctggcgccc gtcctgaagg acctgccgac ggggtacttc
    12421 gtcgagctcg gcgggcagtt cgagaaccag cggcgcgcga tgcggcagct cgcggtcgtc
    12481 gtcccgatgg tgctgctgct gatcctcttc ctcctctact cggcgctcgg cagcgcccgg
    12541 gacgcggccc tcgtcctcct gaacctcccg ttcgccctcg tcggcggcgt cgtcgccgcg
    12601 gtcgccttcc ggatgacgct ctcggtctcg gccgccgtcg ccttcatcgt cctcctcggg
    12661 atcgccgtgc agaacggcgt cgtcctcgtc gcgttcttca cgcagctgcg cgcgcgcggc
    12721 cgggaggccg gcgccgcggt cgccgagggc tgccggctcc ggttcaagcc gctcgtgatg
    12781 accgccctca cgagcttcat cggccacctg ccgatggtct gggcctcagg ctccggcgcc
    12841 gacatccaga agccgctcgc ggtcgtcgtc atggggggca tcgtcacgtc gacgctcctg
    12901 acgctgctcg tccttcccgt cctctggcac gccctcgaga agcggacggg cgccgcgccg
    12961 gcggccgcat gacgccgcgc cccgggccgc ccccgggagg gctcacggaa gcggcggcgg
    13021 cacgcggcgg cgggtcgcct gctcgaaggc gtaggcgagg cggaggagcg tcggctcgct
    13081 gcaggcccgg cccgcgaagc tgacgccgta cggcgcgggc ttcgcctcga agccgtcggg
    13141 gaacggcggc ttcggggcgt tcgggaccgt cccgaacggg acgatgacgg tcgggtagcc
    13201 gggccgcgcg gagatcgagg cgccggaggg gccggggaag aggagcgcgt cgaggcgctc
    13261 cgccgccatc accgcgtcga ggccgtgggt cgccgtcagg aggaggtcct tcgcccggtc
    13321 ggcctcgtag cgcgcgcggt cggccgcgag gtcgagggag tcggagaagt cgaggatcga
    13381 ctggccgtac cggatcgcgc ccgccttctc gtgcgcgacg ttccactccc ggagggcgcg
    13441 gagcgacttc accggcgcgc gctccccgag cgaggcgagc caggcgttga aatcgcgctc
    13501 catcccgtag gcgaggacga tggagcactc cttctccttc atctcgtccc ggtcggtgca
    13561 gacgccccag cggaggtagt tccgcgaggg gtccgggtcg acgacgctcg ggacgtcggc
    13621 cgggtcgacg atgacggcgc cttccttctt cagggcctcg atcgcctcgg ccatcacctt
    13681 cgcctgctcc gggctcaggc cgccgcgcgg cttctccgcc ccgggaggcg tgaccctgtc
    13741 gtagtagaag gcccgcggga tgccgatgcg cgcgcccttc aggccgtcct tcttcaggaa
    13801 ccgcgtgtag tcgttcccgg gcggcggcgc gcaggccgac gtggccggat cgttcgggtc
    13861 cggagccgtc ccctcgagga cgccgaggag gatcgccgcg tccgtcacgg agcgggccat
    13921 cgggcccggc gtgtcctggt cggccgtgat cgggatgacg ccggtccggc tgatgcgccc
    13981 cacggtcggc ttgatcccga cgagcatgct ctggttcgag ggggagagga tcgagcccga
    14041 ggtctcggtg ccgacgctcg cggcccagaa gctcgcggcc gtccccgcgc cggagctcga
    14101 gccgccggtg ttcagcgcgg gccggccgtc gaacgtcgcc tcgcgcgggt cgcgccgcgc
    14161 gtcgtacggg ttgaagcccc agccgttcag gccgttgtag ttgttcggca tcggctgcgg
    14221 cgaggcggcg acccagttcg cgagctcggt gagctgcgtc ttggcgagga tcaccgctcc
    14281 ggcggcccgg aggttcttca cgagcgtggc gtccgccggc ggcacccagc cgtcgaaggc
    14341 gagcgctccg cccgtcgtcg gaaggtcggc ggtctggacg ttgtccttca cggcgacggg
    14401 aatgccgtgg agaggcccgc gcactctccc cgccttccgc tcgcggtcga gcgcgtcggc
    14461 ctcggcgagg gcgcgcgggt tgacggtgat cgcggcgtgg agccggccct cccacgtcgc
    14521 gatccgcgcg agcgagagaa ggacgaggtc gcgcgaggtg acgcgcccct ccttcatcgc
    14581 ggcctgcatc gccgggatgg tgctctcgac gacgtcgaac ggctcggccg cgccgaggcg
    14641 caggggagcg aggaggagga gggcgaggag caggcgtccc gtcgtcatcg agtcgactcc
    14701 ctccgggcgc gttcggtccg cggcacggga ggcgagttga tcacgttccg cgccctcgcg
    14761 gccgccgaat ctcccgcggc gcccgacgcc gacctctcgc cctccgcgct cctcgcgtgc
    14821 gccctcgtcc gcgcctcggg gcacgccggg ggggacgatg gtccacggcc cccgggctcc
    14881 ctccgcaggc ggcgagccct cccgttgact cccctcgtgc ccgcgcatac ggtgtccggc
    14941 cggagggacc ccatgagcca cccgcgcgcc gggcagcccg ccgagggccg agacctcgtc
    15001 cacgtcccgc gcctcctcgc cgcctactac accgagcggc ccgacccggc cgagcgcgcc
    15061 cagcgcgtcg cgttcgggac ctcgggccac cgcggctcct cgctgcgcgg gagcttcaac
    15121 gaggcgcaca tcctcgcgac gacgcaggcg atctgcgagg tccgcgccgc gcagggggcg
    15181 accgggccgc tcttcctcgg ccaggacacc cacgctctct ccgagccggc cttccggacg
    15241 gcgctcgagg tcctcgcggc gaacggcgtc acggtgcgcg tcgacgcggc cgacggcttc
    15301 acgccgacgc ccgtcgtctc gcacgcgatc ctcgcgtgga accgggacca cgacggcccg
    15361 cgcgccgacg ggatcgtcat ctcgccgtcg cacaacccgc cggaggacgg cggcttcaag
    15421 tacaacccgc cgcacggcgg gccggccgac accgacgcga cgaaggcgat cgagagccgg
    15481 gcgaacgccc tcctcgccgc cgggctcgag ggggtgaagc gggttccctt cccgcgcgcg
    15541 cggaaggccg cgacgaccgt cgcgcacgac tacctgacct cgtacgtcga cgacctcgcg
    15601 tcggtcgtcg acctcgaggc gatccgcgcg gcgggcgttc ggatcggcgt cgacccgctg
    15661 ggcggcgcct cggtcgactt ctggcagagg atcgccgagc ggtggcggct cgacctgacc
    15721 gtcgtcaacc cgcgcgtcga cccggcgttc gcgttcatga cgctcgactg ggacggccgg
    15781 atccggatgg actgctcctc gccgtccgcg atggcctcgc tcatcgcgat ccgcgaccgc
    15841 taccaggtcg ccgtcgggaa cgacgccgac gtcgaccgcc acgggatcgt gacgccctcc
    15901 ggcctcatga acccgaacca ctacctcgcc gccgcgatcc gctacctctt cggcgcgcgc
    15961 gacggctggc ggaaggacgc aggggtcggc aagacgctcg tctcctcgag cctcatcgac
    16021 cgcgtcgccg aggggctcgg ccggaagctc gtcgaggtgc cggtcggctt caagtggttc
    16081 gtcccggggc tcctctcggg agacctcggc ttcgggggcg aggagagcgc gggggcttcc
    16141 ttcctccggc gcgacggaac gacctggacg accgacaagg acgggatgat cctcggcctc
    16201 ctcgccgcgg agat
//
LOCUS       DAOTEZ010000040        16113 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2459_length_16113_cov_6.913875, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000040 DAOTEZ010000000
VERSION     DAOTEZ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 16113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16113
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2459_length_16113_cov_6.913875"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1008..1349)
                     /locus_tag="P5164_03420"
     CDS             complement(1008..1349)
                     /locus_tag="P5164_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRY42977.1"
                     /translation="MEMKLYVGNLPYTMDDATLRGLFEGFGEVHSARVVSDRDSGQSK
                     GFGFVEMNDSEAQSAMSALNGQEHGGRTLRVNEARPQEARTGGGGGGRGGYGGGGGYG
                     GGGYGGGRGRY"
     gene            complement(1553..3088)
                     /gene="guaA"
                     /locus_tag="P5164_03425"
     CDS             complement(1553..3088)
                     /gene="guaA"
                     /locus_tag="P5164_03425"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRY42978.1"
                     /translation="MPSFDTVVVLDFGSQYTQVIARRIREARVRSIVLPYSAPAAEIE
                     ALAPKGIVLSGGPSSVYDERAPRGDERVFDLGIPVLGLCYGMQLMALHYGGGVGRAPG
                     REYGRAVVDVAGGRLLGALGPRETVWMSHGDHVETVPDGFTVTARTANAPVAAFEDPN
                     RALYGLQFHPEVHHTDHGAEMLEAFLYGVCGCAPGWTMASFRESAVAKIRETVTEGVV
                     LGALSGGVDSTVAAVLIREAVGERFRGVFVDTGLLRKDEGRHVLAAFGHLGLPVTGVD
                     ASERFLSALAGVTDPERKRKIIGGLFIDVFAENAKAVDGAAWLAQGTLYPDVIESVSI
                     HGPSAVIKTHHNVGGLPAKLGFRLVEPLRELFKDEVRRLGEELGIPRDMLYRHPFPGP
                     GLAVRIPGEITPERVRILQEADAIFMEELRASGDYDRTAQAFAVLLPVRTVGVMGDGR
                     TYEHVVALRAVTTIDYMTADWARLPHDLLDRASRRIVGEVRGVNRVVYDVTSKPPATI
                     EWE"
     gene            complement(3097..3657)
                     /locus_tag="P5164_03430"
     CDS             complement(3097..3657)
                     /locus_tag="P5164_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42979.1"
                     /translation="MFRTVRGFLMLAPPAFLAALIAYLSAGPRAATSFTVYPFLVDVL
                     GSGLPRSNVARFVVVTTLFFIVPYLVSGVLLLLADIGSSAAASLWRRERRTPAAGQRP
                     RLLAPEAFWTLVVAAVLLAAAAGSQLPKVAHGGELPGGVNVAPVFVAAVPFVALACAL
                     ALAAIASIPRAVARRIGPRQRSDGGL"
     gene            complement(3683..4378)
                     /locus_tag="P5164_03435"
     CDS             complement(3683..4378)
                     /locus_tag="P5164_03435"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019626438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRY42980.1"
                     /translation="MRRLLASALLALLLPAAPAASALVVLTEGRHLKAASFELQGEEK
                     IRVVLVGGGEIVLPIERVERIVDDELEPFAAPEEPAAPAPKAWPSVRQSSGRPHSAPA
                     RWARLVEEAAEAHQLDPALVAAVIHVESSWKPRAVSPKGARGLMQLMPATARRLGVSR
                     SFDPVQNVGGGARYLSLLAKRFGENEVEKILAAYNAGEGAVESYGGIPPYRETRAYVR
                     KVLALWTGEPAGS"
     gene            complement(4371..5294)
                     /locus_tag="P5164_03440"
     CDS             complement(4371..5294)
                     /locus_tag="P5164_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HRY42981.1"
                     /translation="MEASPTETRPSEFFLRDLPLFLDHLAVEKGLARNSLEAYRRDLT
                     AFGRHLDAEGLSAPDVTRDVLVRWFGLCRSAGASPRSIARATSALRGLFRFLFAEKRI
                     AADPTSGLENPRRWMTLPKLLGRDEVERLLEAPDVETPKGLRNKAMFELLYACGVRVS
                     ELATLRVGSLHLADGFVVVKGKGSKERVVPVADSSARWVVRWMAGPRARGRAAASPWL
                     FPGTGGKPVTRQTIFLAIKAAARKAGLDPAAVSPHVLRHSFATHLVDGEADLRAVQMM
                     LGHASIATTEIYTHVSRARARRVYDRSHPRA"
     gene            5385..6548
                     /gene="hppD"
                     /locus_tag="P5164_03445"
     CDS             5385..6548
                     /gene="hppD"
                     /locus_tag="P5164_03445"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HRY42982.1"
                     /translation="MSQDLGIVGYDSFHFIVGDLERSRRFYTEALGFTEVARASRAKV
                     EAGGEEASVFGAGQIRVLVSTPVRPDSRAARWLRVHPDGVSSLAFRVRDVDAAWRFLE
                     PRGATFLTDVQVHEEGGGYFRTFSIATPLDDVTFRFIERRGTYPGFAPDFENVGDGVP
                     SDNPHGFATIDHVTSNAYTMLPVVNWYRDVLGFSRYWDVEFHTSEVDGGKKMTGSGLR
                     SIVMADPSSGIKFATNEPMRPFFRDSQINRFCEDHHGAGVQHVALLVKEILPAVESLR
                     SRGVQFLSAPPAYYDRLPERLERVKVSNVKEPMDALRANHVLVDGKDDRYMLQIFMHD
                     AGTLYGDVKAGPFFYEIIQREGDKGFGYGNFRALFESIEAAQSADSSSLDVVG"
     gene            6550..7707
                     /locus_tag="P5164_03450"
     CDS             6550..7707
                     /locus_tag="P5164_03450"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HRY42983.1"
                     /translation="MIDRMQLGDVPAKPHVAFRSASGALRFEECFTRLGFDGEYSILY
                     HEGPPMTDRSIGPAEPGPLDRRVPAWAPDEPLRRRLFLGDGTPQGWTPVCGNADVVVS
                     LFDLPAAPAPETGFANGDGDDLFYVFSGRATLETPFGDLAAGAGDYVLVPRSVVHRWR
                     VDERITGIAFELRGGLHVPTQYRNAVGQLRMDAPFTHRDFRRPVFPGPRPGPREVLVK
                     KGDAFVRREPLVSPFDVVGWDGTVWPFAFPILAFQPKIGLVHLPPTIHATFAARGALV
                     CSFVPRVTDFHPEAVPCPYPHSSVDCDELIYYVRGNFTSRRGVGPGAVSLHPAGIPHG
                     PHPGAYEASIGTRSTDELAVMIDTFLPLRVTEQARALELPEYHDSWLPKEP"
     gene            7704..9005
                     /locus_tag="P5164_03455"
     CDS             7704..9005
                     /locus_tag="P5164_03455"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRY42984.1"
                     /translation="MTSSLRIGLLGYGAVGKGLVRLLASETPRLAEEHGLDLRLVAVA
                     SRALPRRPELLASIPPGVRVGDDLSAVALAPDVDVVVELLGGLEPAGTLVESALRAGR
                     SVVTANKLLLAARGAELARLARETGAALAFEAAVAGGIPVLRALRESFVSDQIEEVGG
                     ILNGTSNFVLTEMAQTARPYGEVLAEARSLGYAEADPTADVEGTDAACKVALLARMAF
                     GVDAPLEAVRTEGVTRLRPIDFVYARLLERTPRQLGLARRLPDGRFLLFVRTVLVPAS
                     SLLARIDGPLNAVRVTTAHGGAYVLTGPGAGGAPTATAVLADVVEIARRGVPSPVPPF
                     GTDEPRPCLSGGPADFVAPFYVRFLVDDRPGVLAALTTAFARAEVNVDAVLQVPWAEK
                     RALPFVVTLEATEEERVTRALAEIAEQPFLVEAPLLLPLVA"
     gene            complement(9024..9887)
                     /locus_tag="P5164_03460"
     CDS             complement(9024..9887)
                     /locus_tag="P5164_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42985.1"
                     /translation="MKVKLGATLLVGMLMLAQGEVQAQGKPASAVKKFRFKGGKIETG
                     RLYAYDRFDPAGGRAGSVLVYVPDRKRVEIFRAKTGAGEGQAFSGEMNWDTFTLRSFE
                     VSRVSRDGTTTRQAAGTFGDGALAVSVEDPALYRGAEGAATFTVPLPAIPAHLYSLDL
                     ITLGLALRHVADPSGSVEVGVLTENPKVGPQSPNLFVAAGTATVEFVEDVTRDGVSCG
                     KYRVSGPALGGQEGFVWLHKDEGYLQDAEIPVSPTPDLPDVKITLRSVEKLSEKDWPA
                     RRLREISGVPR"
     gene            10056..10544
                     /locus_tag="P5164_03465"
     CDS             10056..10544
                     /locus_tag="P5164_03465"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HRY42986.1"
                     /translation="MAIKLLITGGTFDKDYDELTGKLYFRDTHIHEMLKLGRCRLQTR
                     VRTVMMVDSLEMTDSDRKIIEEICCKSEEEQIVITHGTDTMEETARVLGAVGLPKTIV
                     LTGAMIPYAFGSSDGLFNLGSALSFVQVLPHGVYVAMNGTYFPWNDVRKNRGLGVFEK
                     LS"
     gene            10573..11154
                     /locus_tag="P5164_03470"
     CDS             10573..11154
                     /locus_tag="P5164_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY42987.1"
                     /translation="MRGVEQVPLLYDAGLALLEATGLGRWRRWLASGADGRVLDLGCG
                     TGRNLPLFSAAIRAVGLDPCLETLRAARRRSPDTPLVRAVAERLPFRPGTFDTVVSGL
                     VFCSVTDPAGAFGEVRRVLAPCGRLRMLEHVRADEPWHARIQDAVQPLWTRLTGGCRP
                     NRDTVAAVRAAGFRLEEGSYRASGTMRRLVARP"
     gene            complement(11219..11602)
                     /locus_tag="P5164_03475"
     CDS             complement(11219..11602)
                     /locus_tag="P5164_03475"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRY42988.1"
                     /translation="MEVRFPGGVAVEAIHAGMTIRTDQPVAAGGAGSAPSPFDLFLVS
                     LSTCAGFYALRFCQERGLPTEGLGVTMDWERSPETKLISKIRISVKLPEGFPEKYRAA
                     ILRATDQCAVKKHLVSPPEIEVEAV"
     gene            11765..13183
                     /gene="glnA"
                     /locus_tag="P5164_03480"
     CDS             11765..13183
                     /gene="glnA"
                     /locus_tag="P5164_03480"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HRY42989.1"
                     /translation="MFSDFSEAKSFVEKRSIEMVDLKFCDLWGRWHHVTIPASGFTPV
                     LMEKGVGFDGSSVGFKSVSSGDMALVPDLATGFLDPFWEVPTLSFICTTLEADTRALF
                     PYDPRSIARRAEEFLRASGVADESVWGPEFEFYVFDGVSYENGMNVAAYRVQSSEGDW
                     NSHELGSGYNIPRHGGYHAIPPQDRLFNFRTRVCKLLSQMGVEVKYHHHEVGGPGQCE
                     IEIPIFPMMKAADAVMIVKYVTRMAATQLGLTATFMPKPLYGEAGSGMHFHQRLLKGG
                     KNLFYDAQGYGSSSAAERSYIAGLLQHGGAVMAFTNPSTNSYRRLVPGYEAPISAIYS
                     LGNRSAAIRIPKYANRPDNARFEFRPPDATCNPYLAIAAQLLAGVDGIRKGLDPTALG
                     FGPVDDDIFSWDAEKRATIKALPTSLEAAMEALESDRAFLLEGDVFNDDLLERWAKKK
                     RSEDREVRNRPHPYEIELYYGL"
     gene            complement(13263..>13957)
                     /locus_tag="P5164_03485"
     CDS             complement(13263..>13957)
                     /locus_tag="P5164_03485"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42990.1"
                     /translation="VLAFKAGKTPLAAKATQSHTASLAGDYAVAEALMKGAGVVVAET
                     LDMLEDYTKAFTMLYDRLPRGNRVAVLSNAGFECSSVLDKLYGLVPAPLTDETKAKLE
                     ACLPGVGHAGNPADATPMATTAQFVAAAQAMVDDPNVDLLLVSPVPVTPALDNLAPDL
                     SGTHRENIHSPGSLPNGLLALFRGTTKPVAVAVDSGRLYDDFVFVLQRGGIPVWRKID
                     RASRALSALATL"
     assembly_gap    13958..14057
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14058..15391)
                     /locus_tag="P5164_03490"
     CDS             complement(<14058..15391)
                     /locus_tag="P5164_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRY42991.1"
                     /translation="MKQILSRARGAGRSALLEHEVYEVLRAAGLDVPRFAFWDGEPAG
                     TVPADVAALLTGGTAEVVLKIVSPQILHKSDVGGVAIVAAEPAAVAAAARRVWDDVGR
                     RMQEATREGVLVVEKVKTKGGSPASETLLSLKQDPAFGPVLVLGLGGVLTEWFGAATG
                     GTSTVVLQPGRVREGLERAVATRPAAALLFRPSRLHERPPLDLAATAERLERLGAVAT
                     AFGEGAQAEEADPITLEELEVNPLLLAADGRWIAVDGKGSLGRSTATLPKRPLRKVKN
                     LLEPKSAAVLGASATAMNPGRIILRNLKLSEGVRYGHIWAVHPKEEAIDGIPCVKTVA
                     ELPQKVDVAVVSIPAEGARDAIRALCSTGKAESIILIPGGFAETGKKALEEEIVSILR
                     ASRSRADEGPVLVGGNCLGIVSKHQYNTFFLPQYKLPFHDAPGDRLLAVSQSG"
     gene            15450..>16113
                     /locus_tag="P5164_03495"
     CDS             15450..>16113
                     /locus_tag="P5164_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRY42992.1"
                     /translation="MAPTDEKRLQDLRTLLEVAQAMTGVRQLQPLLELIVASASRLVD
                     ADRTSLFLLEPDGSLWTRVAQDSSDIRLPPGFGIAGIVARTGETIAIPDAYEDPRFSR
                     DVDQKTGFRTRSILCVPLVTPSGSVVGVIEAMNKKGNAAFDADDAEALRALGSHAALA
                     LETQRLLEGELRRERMESELELARKIQEGLRPRALPATPGLRLAAWQETAEKTGGDYY
                     DVM"
BASE COUNT         2318 a         5899 c         5511 g         2285 t
ORIGIN      
        1 ggcacagtgc ataagctcct cgaatccgcc ttcgcggtga accgacctct cgccggtctc
       61 caccggctct gcgatgacct tcccgaggaa ttaggcaatt cgactggacc gttcgttgtg
      121 cctgccccag tcatctcgac aggatccctt cgactctcgg tcacaggagg cctgcgcaag
      181 gcgggtccga cgtgtcgccg cgcagagacc gtttcggcgc ccttcagggc gcccatggga
      241 cggctcgcgc gcgcaccagc caccatcgcg ttgtcgtttc gcacgggaga cgcgactcta
      301 gtctggaatc ggttctccaa tcaagcaatt agcgaatttc gagaatcact cgcaagcgaa
      361 caaaacatac cgggtgtttt gtattcgcac gtcaggactc gaacggaggc aggcgtctcg
      421 agacgaggcc cgggtacgaa ctcaggtttc cggactgccg caggaggtca tcgccatgac
      481 gcagaccacg cgaaccggct ctggctcccg cgttgaaacg tcccgcgcgc gagtcgcgat
      541 ccggcctccg ttccgtaggt cgtagctggc atacgctcga cgccccagag agcgaccgca
      601 atccgtgacg ggcacgcgcg aaactttcgg ccacgcggac ggcaaggagg tcgcgccgcg
      661 aaagcctcgc gacctcgcgc gcatcgaaaa cgggccgatc gggcacgcga tgaggtcgtc
      721 cctctgtagg gtttcgaggg gatcggcgtt ctcctcgtcg cgccgaaccg ccgatggcga
      781 tcccacgacc cggaaggcgg tcgccgcgcg gacgacgttc ggatcgccga acccggccgc
      841 gcacgcagag gacgtcggcg gcgactccag agtgaagcgc gacggtcccg gcaaccgccg
      901 ccggcagcgt cggctccaac tcccggagca gtggcgcgaa gcgagacggg gaggacggat
      961 cgctccgccc tccccggccc gaggtgtcag gtttctaccg ggacggacta gtagcggccg
     1021 cggccgccgc cgtagccacc accgccgtag ccaccgccgc cgccgtagcc gccacggccg
     1081 ccgccgccgc cgcccgtgcg ggcttcctgc gggcgggcct cgttgacgcg cagggtccgg
     1141 ccgccgtgct cctggccgtt cagggcgctc atcgcgctct gggcctcgct gtcgttcatc
     1201 tcgacgaatc cgaagccctt cgactggccg gaatcgcggt cgctcacgac gcgggcggag
     1261 tggacttccc cgaagccctc gaacaggccg cggagggtgg cgtcatccat cgtgtagggg
     1321 aggtttccga cgtaaagctt catttcgatc tcgaggtcct tgtttcagtg cgcgccgagg
     1381 cgagcggttt tgggagtcgg cgcgattccg agccgacggg aagcgagttg tcaccgtcct
     1441 caccgccagt cggagacgga acgggtccct ttcagcacaa cacggtcgcg accggttgtc
     1501 aactgccgac cggagggcgg ccaccgggga aggtacgaag cgctccccgg gctcactccc
     1561 actcgatcgt cgccggcggc ttggaggtca cgtcgtagac gacccggttc acgccgcgga
     1621 cctctccgac gatgcgtcgc gatgcccggt cgaggaggtc gtgagggagg cgggcccagt
     1681 cggccgtcat gtagtcgatc gtcgtcaccg cccggagcgc gacgacgtgc tcgtacgtcc
     1741 ggccgtcgcc catcactccg acggtgcgga ccggcaggag gacggcgaaa gcctgcgccg
     1801 tccggtcgta gtcgcccgag gcccgcagct cctccatgaa gatcgcgtcc gcctcctgga
     1861 ggatccggac gcgctcgggc gtgatctcgc ccgggatccg caccgcgagc cccggcccgg
     1921 ggaacggatg ccggtagagc atgtcgcgcg ggatgccgag ctcctcgccg aggcgccgca
     1981 cctcgtcctt gaagagctcc cggagcggct cgacgagccg gaacccgagc ttggccggga
     2041 ggccgccgac gttgtggtgc gtcttgatga cggccgaagg cccgtggatc gagaccgact
     2101 cgatcacgtc gggatagagg gtcccctgcg ccagccaggc cgcgccgtcc accgctttcg
     2161 cgttctcggc gaagacgtcg atgaagaggc cgccgatgat cttccgcttg cgctccgggt
     2221 cggtcacgcc cgcgagcgcg gagaggaagc gctcggaggc gtcgacaccc gtgacaggga
     2281 ggccgaggtg cccgaaagcc gccagcacgt ggcgcccctc gtccttgcgg aggaggccgg
     2341 tgtcgacgaa gaccccccgg aaccgctccc ccacggcctc gcggatcagg acggcggcga
     2401 ccgtggagtc gacgccgccg gagagggcac ccaggacgac gccttcggta accgtctcgc
     2461 ggattttcgc gacggccgac tcgcggaagc tggccatcgt ccatcccgga gcgcatccgc
     2521 agactccgta gaggaatgcc tcgagcatct cggcgccgtg gtcggtatgg tggacctcgg
     2581 ggtggaactg gaggccgtag agcgcccggt tcgggtcctc gaaggccgcg accggggcgt
     2641 tcgccgtccg cgccgtcacc gtgaacccgt ccggcacggt ctccacgtgg tcgccgtggc
     2701 tcatccagac ggtctcccgc ggtccgagcg ccccgaggag ccgtccgccc gccacgtcca
     2761 cgacggcacg gccgtattcc cgccccggcg cgcggcccac gccgccgccg tagtggagcg
     2821 ccatgagctg catgccgtag cagaggccga ggaccgggat cccgaggtcg aagaccctct
     2881 cgtcgcctcg cggcgccctt tcgtcgtaga cgctcgaggg cccgccggaa aggacgatcc
     2941 ccttcggcgc gagggcctcg atctccgcgg cgggggcgga gtacgggagg acgatcgagc
     3001 ggacccgggc ctcgcggatc cggcgggcga tgacctgggt gtactgggag ccgaaatcga
     3061 ggacgacgac ggtgtcgaag gacggcatcg tctcgatcat agcccgccat cggaacgctg
     3121 ccgggggccg atccgccgcg cgacggccct cggaatcgag gcgatcgccg cgagggcgag
     3181 ggcgcaggcg agcgcgacga acggcacggc cgcgacgaag acaggtgcca cgttcacgcc
     3241 gcccgggagc tccccgccgt gcgccacctt cgggagctgc gagccggccg ccgccgccag
     3301 gaggaccgcg gccacgacga gcgtccagaa cgcctcgggc gcgaggagcc tcggccgctg
     3361 tcctgccgcc ggggtccgcc gctcccgcct ccagagcgag gccgcggccg agctgccgat
     3421 gtccgccagg aggaggagca cgcccgagac gaggtaaggg acgatgaaga agagcgtcgt
     3481 gacgacgacg aaacgggcca cgttcgagcg cgggaggccg gagccgagga cgtcgaccag
     3541 gaacgggtag acggtgaacg aggtcgcggc ccgaggcccg gcggagaggt aggcgatcag
     3601 cgcggccagg aaggccggag gcgcgagcat caggaatccg cgaaccgtcc ggaacacgcg
     3661 ggcgagtata ggaggccggg cgtcaggagc cggccggctc gcccgtccag agggcgagga
     3721 ccttcctcac gtaggcgcgc gtctcgcggt acggcgggat cccgccatac gactcgacgg
     3781 ccccctcgcc ggcgttgtag gccgccagga tcttctccac ctcgttctcg ccgaagcgct
     3841 tcgcgaggag cgagaggtaa cgcgcgccgc cgccgacgtt ctggaccggg tcgaacgagc
     3901 gggagacgcc cagccggcgc gcggtggcgg gcatgagctg catcagcccc cgggctccct
     3961 tcggcgagac ggcccggggc ttccagctcg attcgacgtg gatcacggcc gcgacgaggg
     4021 ccgggtcgag ctggtgggcc tccgccgcct cctcgacgag ccgggcccag cgagcggggg
     4081 ccgaatgcgg ccgtcccgac gactggcgca ccgagggcca cgccttcgga gcgggagcgg
     4141 ccggctcctc gggcgcggcg aacggctcga gctcgtcgtc gacgatccgc tcgacccttt
     4201 cgatcgggag gacgatctcg ccccctccga cgaggacgac ccggatcttc tcctctccct
     4261 ggagctcgaa cgaggccgcc ttcaggtggc gcccttccgt caggacgacg agcgcggagg
     4321 cggccggcgc ggccgggagg aggagggcca ggagcgccga cgcgaggagc ctacgcacgg
     4381 ggatggctcc tgtcgtagac gcgccgggcc cgggcccgcg agacgtgcgt gtagatctcg
     4441 gtcgtggcga tgctcgcgtg cccgagcatc atctggacgg cccggaggtc cgcctcgccg
     4501 tcgacgaggt gggtcgcgaa cgagtggcgc aggacgtgcg gggagaccgc cgccggatcg
     4561 aggccggcct tccgcgccgc ggccttgatc gccaggaaga tcgtctgccg cgtcaccggc
     4621 tttccgcccg ttcccgggaa gagccacggc gaggccgccg cgcgccctcg ggcgcggggc
     4681 ccggccatcc agcggacgac ccagcgtgcc gaggagtcgg ccacggggac gacccgctcc
     4741 ttcgagccct tccccttcac gacgacgaaa ccgtcggcga ggtggagcga gccgacccgg
     4801 agcgtcgcga gctcggagac ccggactccg cacgcataga ggagctcgaa catcgccttg
     4861 ttccgaagcc ccttcggggt ctcgacgtcg ggcgcctcga ggaggcgctc cacctcgtcg
     4921 cggccgagca gcttcgggag agtcatccag cgccgcgggt tctcgaggcc ggaggtgggg
     4981 tcggcggcga tccgcttctc ggcgaagagg aagcggaaga gaccgcgcag cgccgaggtc
     5041 gccctcgcga tcgaacgggg cgacgccccc gctgaccggc agaggccgaa ccacctcacg
     5101 aggacgtccc gggtcacgtc cggtgccgag agcccctccg cgtcgaggtg gcggccgaag
     5161 gcggtcaggt ctctccggta ggcctcgagc gagtttcggg ccagcccctt ctcgaccgcg
     5221 aggtggtcga ggaaaagggg caggtcgcgc aggaagaact cgctcgggcg cgtttccgtc
     5281 gggctcgcct ccacctcccg attctacgga gccggggccc gcggcgtcgg gtcgccggac
     5341 cggctgcgct cccgcccgca ccgttccctc ctagaatccg tctcatgagc caggacctcg
     5401 gcatcgtcgg atacgactcg ttccacttca tcgtcggcga cctcgagcgg agccgccgct
     5461 tctacacgga ggccctcggc ttcacggagg tcgcgcgcgc ctcgcgcgcc aaggtcgagg
     5521 ccggaggcga ggaagcgagc gtcttcgggg ccgggcagat ccgcgtcctg gtctcgacgc
     5581 cggtccgtcc cgactcccgg gccgcgcgct ggctccgggt ccaccccgac ggcgtctcct
     5641 cgctcgcgtt ccgggtccgc gacgtggacg ccgcctggag gttcctcgag ccccggggcg
     5701 ccacgttcct gaccgacgtc caggtccacg aggagggggg cggctacttc cggacgttct
     5761 cgatcgcgac gccgctcgac gacgtcacgt tccggttcat cgagaggcgg gggacgtatc
     5821 ccggattcgc ccccgatttc gagaacgtcg gcgacggcgt tccgtcggac aaccctcacg
     5881 gcttcgcgac gatcgaccac gtcacctcga acgcctacac gatgctcccc gtcgtcaact
     5941 ggtaccgcga cgtcctcggc ttctcccgct actgggacgt cgagttccac acctcggagg
     6001 tcgacggcgg gaagaagatg accggctcgg gcctccgctc gatcgtcatg gccgacccgt
     6061 cgagcgggat caagttcgca acgaacgagc cgatgcgccc cttcttccgc gacagccaga
     6121 tcaaccggtt ctgcgaggac caccacggcg cgggcgtcca gcacgtcgcc ctcctcgtca
     6181 aggagatcct cccggccgtc gagagcctcc ggtcgcgcgg cgtccagttc ctctccgcgc
     6241 ctcccgccta ctacgaccgg ctcccggaga ggctcgagcg cgtaaaggtc tcgaacgtga
     6301 aggagccgat ggacgccctt cgcgcgaacc acgtcctcgt cgacggcaag gacgaccgct
     6361 acatgctcca gatcttcatg cacgacgccg gcacgctcta cggcgacgtg aaggcgggcc
     6421 ctttcttcta cgagatcatc cagcgcgaag gggacaaggg cttcggctac gggaacttcc
     6481 gcgctctctt cgagtcgatc gaggccgcgc agagcgccga ctcgagctcc ctcgacgtgg
     6541 tcggttgact tgatcgaccg gatgcagctc ggcgacgtgc cggcgaaacc ccacgtcgcg
     6601 ttccgctcgg cttcgggcgc gctccgcttc gaggagtgct tcacccgcct cggcttcgac
     6661 ggcgagtact cgatcctcta ccacgagggc ccgccgatga ccgaccggtc gatcgggccc
     6721 gcggagccgg gaccgctcga ccgccgcgtc cccgcgtggg ctcccgacga gcctcttcgc
     6781 cgccggctct tcctcggcga cgggacgccg cagggctgga cgcccgtctg cggcaacgcc
     6841 gacgtcgtcg tctccctctt cgacctcccg gccgccccgg cgccggagac gggcttcgcg
     6901 aacggcgacg gggacgacct tttctacgtc ttctccgggc gggccacgct cgaaactccg
     6961 ttcggcgacc tcgccgcggg cgcgggggac tacgtcctcg tcccgcggag cgtcgtccac
     7021 cggtggcgcg tcgacgagcg aatcaccgga atcgcgttcg agctccgggg cggcctccac
     7081 gttccgacgc agtaccgcaa cgccgtcggc cagctccgga tggacgcgcc gttcacgcac
     7141 cgcgacttcc gccgtccggt ctttcccggg ccgaggcccg gaccgaggga ggtgctcgtc
     7201 aagaagggcg acgccttcgt ccgccgcgag cctctcgtct cgccgttcga cgtcgtcggc
     7261 tgggacggca ccgtctggcc cttcgcgttc ccgatcctcg cgttccagcc gaagatcggc
     7321 ctcgtccacc tcccgccgac gatccacgcg acgttcgccg cccgcggggc gctcgtctgc
     7381 tcgttcgtcc cgcgggtgac cgacttccac cccgaggcgg tcccctgccc ctatccccac
     7441 tcgtcggtcg actgcgacga gctgatctac tacgtgcgcg ggaacttcac gagccgccgc
     7501 ggcgtcgggc cgggagcggt ctccctccat ccggccggga ttccgcacgg cccgcacccc
     7561 ggcgcctacg aggcgtcgat cggcacccgg tcgaccgacg agctcgcggt catgatcgac
     7621 acgttcctcc cgctgcgcgt cacggagcag gcgcgggccc tcgagctccc cgagtaccac
     7681 gactcctggc ttccgaagga gccgtgacct cctccctccg gatcggcctc ctcggctatg
     7741 gagccgtcgg gaaggggctc gtccgcctcc tcgcgtccga gaccccgcgt ctcgccgagg
     7801 agcacgggct cgacctccgc ctcgtcgccg tcgcgagccg cgcccttccc cgccgccccg
     7861 agctcctcgc ctcgatcccg ccgggagtcc gcgtcggcga cgacctctcc gccgtcgccc
     7921 tcgcgccgga cgtcgacgtc gtcgtcgagc tgctcggcgg gctcgagccg gcgggcacgc
     7981 tcgtcgagtc ggcgcttcgg gcgggccggt ccgtcgtcac cgccaacaag ctcctcctgg
     8041 ccgcccgcgg cgccgagctc gcgcgcctcg cgcgcgagac gggggccgcc ctggcgttcg
     8101 aggcggccgt cgccggcggc atccccgtcc tccgcgcgct gcgggagagc ttcgtctcag
     8161 accaaatcga ggaagttgga gggatcctca acggcacgag caacttcgtc ctgacggaga
     8221 tggcgcaaac ggcccgaccg tacggcgagg ttctcgccga ggcgcgatcc ctcggctacg
     8281 ccgaggccga cccgactgcg gacgtcgaag ggacggacgc cgcctgcaag gtcgcgctcc
     8341 tcgcccggat ggctttcggc gtcgacgccc cgctcgaggc cgtccggacc gagggcgtga
     8401 cccgccttcg gccgatcgac ttcgtctacg cccgcctcct cgagcgaacg ccgcgccagc
     8461 tcggcctggc ccgtcgcctg ccggacggcc gcttcctcct cttcgttcga accgtcctcg
     8521 tcccggcctc gtcgctcctc gcacggatcg acgggccgct caacgcggtc cgcgtgacga
     8581 cggcgcacgg cggcgcgtac gtcctgacgg gtcccggcgc cggcggcgct ccgaccgcga
     8641 cggccgtcct cgccgacgtc gtcgagatcg cccggcgcgg cgtcccgtcc cccgtcccgc
     8701 cgttcggcac ggacgagccg cggccgtgcc tgagcggggg gccggccgac ttcgtcgctc
     8761 cgttctacgt ccgcttcctc gtggacgacc ggccgggagt cctcgccgcc ctcacgacgg
     8821 cgttcgcccg cgcggaggtg aacgtcgacg ccgtcctcca ggtcccctgg gccgagaagc
     8881 gcgcgctccc gttcgtcgtg accctggagg cgacggagga ggagcgcgtc acccgggcgc
     8941 tcgccgagat cgcggagcag ccgttcctcg tggaggcgcc cctcctcctg cccttggtcg
     9001 cctgaccgcc ccggggcgcg gtcctatctc ggtacgcccg agatctctcg gagccggcgc
     9061 gccggccagt ccttctcaga cagcttctcg accgaccgca gggtgatctt cacgtcgggg
     9121 agatcgggcg tcggggagac ggggatctcc gcgtcctgga ggtagccctc gtccttgtgg
     9181 agccacacga agccctcctg accgccgagc gccgggccgg aaacccggta cttgccgcag
     9241 gagacgccgt cgcgcgtgac gtcctcgacg aactcgaccg tcgccgtccc cgcggcgacg
     9301 aagaggttcg ggctctgagg tccgaccttc gggttctcgg tgaggacgcc gacctccacc
     9361 gaccccgagg gatcggccac gtgccgcagg gccaggccga gcgtgatgag gtcgaggctg
     9421 tagagatggg ccgggatcgc cggaaggggg acggtgaacg tggccgcgcc ctccgcgccg
     9481 cggtacaggg ccgggtcctc cacggacacc gccagggcgc cgtcgccgaa cgtcccggcc
     9541 gcctgccggg tcgtcgttcc gtcccgggag actctcgaga cctcgaacga ccggagcgtg
     9601 aacgtgtccc agttcatctc cccggagaag gcctgccctt cgccggcccc cgtcttcgcg
     9661 cggaagatct cgacccgctt ccggtcggga acgtagacga ggacgctccc cgcgcgcccg
     9721 ccggcggggt cgaagcgatc gtaggcgtaa agcctcccgg tctcgatctt cccccccttg
     9781 aatcggaact tcttgaccgc ggaggcgggt ttcccctgtg cctgaacctc gccctgcgcc
     9841 agcatgagca ttccgacgag cagcgtggcg cccagcttca ccttcataca tctcctccgg
     9901 tgagaggccc cctctccaga cggtctccga cctggccgat gcgcggaacg tagcgaatat
     9961 ctccggatcg agcaaggaga atccggccgg ggctcggggc gagggctccg acgcaccctc
    10021 cggcctggct ctatcatcga cggccggggg atccgatggc gatcaagctc ctcatcaccg
    10081 gcggcacgtt cgacaaggac tacgacgagc tgaccgggaa gctctacttc cgcgacaccc
    10141 acatccacga gatgctgaag ctcggccgct gccggctgca gacccgcgtc cggacggtca
    10201 tgatggtcga cagcctcgag atgaccgatt ccgaccggaa gatcatcgag gagatctgct
    10261 gcaagtccga ggaggagcag atcgtgatca cgcacgggac cgacacgatg gaagagaccg
    10321 cgcgcgtcct cggcgcggtc ggactgccga agacgatcgt cctgaccggc gcgatgatcc
    10381 cgtacgcctt cgggagctcc gacggcctct tcaacctcgg gagcgccctc tccttcgtcc
    10441 aggtcctccc gcacggcgtc tacgtcgcca tgaacggcac ctacttcccc tggaacgacg
    10501 tccggaagaa ccgcgggctc ggggtcttcg agaagctctc gtgagccccg ctgccgtgcg
    10561 aagcagccga ccgtgagagg cgtcgagcag gttccgctcc tctacgacgc cggcctcgcg
    10621 ctcctcgaag cgaccgggct cgggcggtgg cgccgctggc ttgccagcgg cgccgacggg
    10681 cgcgtcctcg acctcggctg cggaacgggc cgaaacctgc cgctcttcag cgctgcgatc
    10741 cgcgcggtcg gcctcgaccc gtgcctcgag acgctccggg ccgcccggcg ccgctccccc
    10801 gacacccctc tcgtccgggc cgtcgcggag cgtctcccct tccgccccgg cacgttcgac
    10861 accgtcgtct ccgggctcgt cttctgctcg gtcacggatc ccgccggcgc gttcggtgag
    10921 gtccggcggg tcctcgcccc atgcggccgg ctccggatgc tcgagcacgt ccgcgccgac
    10981 gagccgtggc acgcccgcat ccaggacgcc gtccagccgc tctggacgcg gctcacgggc
    11041 ggctgccggc cgaaccgcga caccgtggcg gccgtccggg cggcaggttt ccggctcgag
    11101 gagggaagct accgggcgag cgggacgatg cggcggctcg tcgcccggcc ctgaacgccc
    11161 cgagaggtgc gccgcgggcg ggtcccccgg cgggccgccg gccggcgctc ccgcgacctc
    11221 agacggcttc gacctcgatc tcgggcggcg agacgaggtg cttcttcacg gcgcactggt
    11281 ccgtcgcgcg gaggatcgcg gcgcggtact tctccgggaa cccctccggg agcttcacgg
    11341 agatccggat cttcgagatc agcttcgtct cgggagagcg ctcccagtcc atcgtgacgc
    11401 cgagcccctc cgtcggcagg ccgcgctcct ggcagaagcg gagggcgtag aagccggcgc
    11461 aggtcgagag agagacgagg aagaggtcga acggggaggg ggccgagccg gccccgccgg
    11521 ccgcgacggg ctggtcggtg cggatcgtca ttccggcgtg gatcgcctcg acggcgaccc
    11581 cgcccgggaa acggacttcc atgggcatcg ttcactccag ggcccgcggc tccggcaggc
    11641 ctcgacgcgg gatggtagca gccggaccga gcctgttcgc gagacaagcc cccgcctccg
    11701 gcgttaccat gaccggccgc gccgcggcct ctcgtgcggc gtgccgaagg gagagagacc
    11761 cgtcatgttc agcgacttca gcgaggcgaa gtccttcgtc gagaagcggt ccatcgagat
    11821 ggtggacctg aagttctgcg acctgtgggg ccgctggcac cacgtcacga tccccgcctc
    11881 gggctttacc cccgtcctga tggagaaggg cgtcggcttc gacggctcga gcgtcggctt
    11941 caagtcggtc agctccggcg acatggccct cgtccccgac ctcgcgaccg ggttcctcga
    12001 cccgttctgg gaagtcccga cgctgtcgtt catctgcacg acgctcgagg cggacacccg
    12061 cgcgctcttc ccctacgacc cgcgctccat cgcccgccgg gccgaggagt tcctccgcgc
    12121 gagcggcgtc gccgacgaga gcgtctgggg cccggagttc gagttctacg tcttcgacgg
    12181 cgtctcgtac gagaacggga tgaacgtcgc cgcctaccgt gtccagtcct ccgagggcga
    12241 ctggaactcc cacgagctcg gcagcggcta caacatcccg aggcacggcg gctaccacgc
    12301 gatcccgccg caggaccgcc tcttcaactt ccggacgcgc gtctgcaagc tcctctcgca
    12361 gatgggcgtc gaggtgaagt accaccacca cgaggtcggc ggcccgggcc agtgcgagat
    12421 cgagatcccg atcttcccga tgatgaaggc cgccgacgcc gtcatgatcg tcaagtacgt
    12481 cacccggatg gcggcgacgc agctgggcct gacggcgacg ttcatgccga agccgctcta
    12541 cggcgaggcc ggctccggga tgcacttcca ccagcgcctc ctcaagggag ggaagaacct
    12601 cttctacgac gcgcagggct acggctcctc gagcgccgcg gagcgctcct acatcgccgg
    12661 tctcctgcag cacggcggcg ccgtgatggc gttcacgaac ccctccacga actcctaccg
    12721 ccggctcgtc ccgggctacg aggcgccgat cagcgccatc tactcgctcg gcaaccggag
    12781 cgcggcgatc cggatcccga agtacgcgaa ccgccccgac aacgcccgct tcgagttccg
    12841 tccgcccgac gcgacctgca acccctacct cgcgatcgcg gcccagctcc tcgcaggcgt
    12901 cgacggcatc cggaaggggc tcgacccgac cgccctcggc ttcggccccg tggacgacga
    12961 catcttctcg tgggacgccg agaagcgggc cacgatcaag gcgctcccga cctcgctcga
    13021 ggcggccatg gaggcgctcg aatccgaccg cgcgttcctc ctcgagggag acgtcttcaa
    13081 cgacgacctc ctcgagcgct gggcgaagaa gaagcggtcc gaggaccgcg aggtgcgcaa
    13141 ccgcccgcac ccgtacgaga tcgagctcta ctacgggctc tgagacccgg cctctcccgg
    13201 aagggcgaag gccccggacg gcacggcgcc gtccggggcc tttccctttt cgcgcccgcc
    13261 ggtcagagcg tcgcgagcgc cgagagcgcg cgggaggcgc ggtcgatctt ccgccagacg
    13321 gggatgccgc cccgctgcag gacgaagacg aagtcgtcgt agagtcgccc cgagtcgacc
    13381 gcgaccgcga ccggcttcgt cgtcccccgg aagagcgcca gcagcccgtt cgggagcgag
    13441 ccgggcgagt ggatgttctc tcggtgcgtg cccgagaggt cgggggcgag gttgtcgagg
    13501 gcgggcgtga ccgggacggg cgagacgagg aggaggtcga cgttcggatc gtcgaccatc
    13561 gcctgcgcgg cggcgacgaa ctgcgccgtc gtcgccatcg gcgtggcgtc cgcggggttc
    13621 cccgcgtggc cgacgcccgg caggcacgcc tcgagcttcg ccttcgtctc gtcggtgagc
    13681 ggcgccggga cgaggccgta gagcttgtcg aggacggagg agcactcgaa cccggcgttc
    13741 gagaggacgg cgacccggtt cccgcggggg agccggtcgt agagcatcgt gaacgccttc
    13801 gtgtagtcct cgagcatgtc gagggtctcc gcgacgacga ctccggcccc cttcatgagg
    13861 gcctcggcga ccgcgtaatc ccccgcgagc gaggccgtgt ggctctgcgt cgccttcgcg
    13921 gcgagcggcg tcttccccgc cttgaacgcg aggacccnnn nnnnnnnnnn nnnnnnnnnn
    13981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14041 nnnnnnnnnn nnnnnnnccg ctctggctga cggccaggag ccggtcgccc ggcgcgtcgt
    14101 ggaacggcag cttgtactgc ggcaggaaga acgtgttgta ctgatgcttc gagacgatgc
    14161 cgaggcaatt gccgccgacg aggaccggcc cctcgtcggc ccgggagcgc gaggcgcgga
    14221 ggatcgagac gatctcctcc tcgagcgcct tcttccccgt ctccgcgaag ccgcccggga
    14281 tcaggatgat cgactccgcc ttcccggtcg agcagagcgc gcggatcgcg tcgcgcgccc
    14341 cctcggccgg gatcgagacg accgcgacgt cgaccttctg cgggagctcg gcgaccgtct
    14401 tcacgcacgg gatcccgtcg atcgcctcct ccttcggatg gaccgcccag atgtgcccgt
    14461 agcggacccc ctcggagagc ttcaggttcc ggaggatgat ccggccgggg ttcatcgccg
    14521 tagcggacgc gccgaggacg gcggccgact tcggctccag gaggttctta accttccgga
    14581 gcggccgctt cgggagcgtc gccgtcgagc ggcccaggct ccccttcccg tcgacggcga
    14641 tccagcgccc gtcggcggcg aggaggagcg ggttgacctc cagctcctcg agcgtgatgg
    14701 ggtccgcctc ttcggcctgg gccccctctc cgaacgccgt cgcgacggcg ccgagcctct
    14761 ccaggcgctc cgccgtggcg gcgaggtcga gcggcgggcg ttcgtgcagg cggctcggcc
    14821 tgaagaggag cgcggcggcc ggccgcgtcg ccacggcccg ctcgagcccc tcgcggaccc
    14881 ggccgggctg caggacgacc gtcgacgtcc ccccggtcgc ggccccgaac cattccgtca
    14941 ggacgccgcc gaggccgagg acgaggaccg ggccgaaggc cgggtcctgc ttgagggaga
    15001 ggagggtctc ggaggccggg gagcctccct tcgtcttcac cttctccacg acgaggacgc
    15061 cctcgcgcgt cgcctcctgc atccgccggc ccacgtcgtc ccagaccctc cgggccgccg
    15121 cggcgacggc cgccggctcg gcggcgacga tcgcgacccc gccgacgtcc gacttgtgga
    15181 ggatctgcgg cgagacgatc ttcaggacga cctcggcggt gcctcccgtc aggagcgcgg
    15241 ccacgtccgc cggaaccgtt ccggccggct cgccgtccca gaacgcgaac cgggggacgt
    15301 cgagccccgc ggcccggagg acctcgtaga cctcgtgctc caggagcgcg ctgcggccgg
    15361 cgccccgggc ccggctcaga atctgcttca ccgtctcgat catcgtgacg aaacccccga
    15421 ccggcgaaag taacatgtcg cgcgacgcca tggcaccgac ggacgagaag aggcttcagg
    15481 accttcgcac gctcctcgag gtcgctcagg ccatgacggg cgtccgccag ctccagcccc
    15541 tcctcgagct gatcgtcgcc tccgcgagcc gcctcgtcga cgccgaccgg acgagcctct
    15601 tcctcctcga gccggacggc agcctctgga cgcgcgtggc gcaggactcc tccgacatcc
    15661 gcctccctcc cggcttcggg atcgccggga tcgtcgcgcg gaccggcgag accatcgcga
    15721 tccccgacgc ctacgaggac ccccgcttct cgcgcgacgt cgaccagaag accggcttcc
    15781 gcacccgctc catcctctgc gtcccgctcg tgacgccgtc cggctcggtc gtcggcgtca
    15841 tcgaggcgat gaacaagaag gggaacgccg ccttcgacgc ggacgacgcg gaggcgctgc
    15901 gcgccctcgg gagccacgcc gccctcgccc tggagacgca gcgcctcctg gagggcgagc
    15961 tgcgccgcga gcggatggag agcgagctcg agctcgcccg gaagatccag gagggcctcc
    16021 ggccgcgcgc cctccccgcg acgccggggc tccgcctcgc cgcctggcag gagacggcgg
    16081 agaagaccgg gggcgactac tacgacgtca tgg
//
LOCUS       DAOTEZ010000041        15963 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2487_length_15963_cov_6.194871, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000041 DAOTEZ010000000
VERSION     DAOTEZ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15963
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2487_length_15963_cov_6.194871"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..1281
                     /gene="queA"
                     /locus_tag="P5164_03500"
     CDS             247..1281
                     /gene="queA"
                     /locus_tag="P5164_03500"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRY42993.1"
                     /translation="MNVSDFDFDLPPEAIAQRPAPRGTSRLMTLDRTTGATGHRNVAD
                     LPGLLLPGDLLVVNDTRVVPARLFGLDETGRRTEFLLVERTGDSSAWDCLAKPGRRAK
                     PGRRFDFGAGLVGTVVSKEESGHYRVAFSGTPLAEALPLVGTTPLPPYIHREGGMADA
                     RDAVEYQTVYAREPGAIAAPTAGLHLTPSLLDALRARGIEVVSVTLHVGIGTFKPVKV
                     ERVEEHRMDPERGVIPEETSVSIRRAKEEGRRVVAVGTTSVRTLEAAAREHGGVVAPG
                     PFETRLFLVPGAEFRIVDALLTNFHLPRSTLLMLVSAFAGRERVLAAYREAVRSGYRF
                     FSYGDAMLVG"
     gene            complement(1309..1869)
                     /locus_tag="P5164_03505"
     CDS             complement(1309..1869)
                     /locus_tag="P5164_03505"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRY42994.1"
                     /translation="MSRRRLIAAALIVTSRLASPAAVAAPPAGAALAPPPAEAVLAAA
                     AKAARAEKKNVLVLFHASWCGWCRKLEAVLAAPGIAEVVGRHFVRAELTVFERGERKE
                     TENAGAETFLESVAGSDAGLPFTAVLDARTRKPIATSNLAGPGTNVGFPSKEEEVDHF
                     VGLLRKGAPRMTSAEEAAVRAAFSPR"
     gene            complement(1925..2251)
                     /locus_tag="P5164_03510"
     CDS             complement(1925..2251)
                     /locus_tag="P5164_03510"
                     /inference="COORDINATES: protein motif:HMM:NF036906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent growth inhibition system
                     immunity protein"
                     /protein_id="HRY42995.1"
                     /translation="MTATTTKKAPATVDVRAYPALRDALRGYLHEDWTTEYADSEDAA
                     RAFRRDAADDEKRSVAPEAKKLRAALEGMEVRELRKLLGAGFGTGWRPSTAAEADRVL
                     RILAGE"
     gene            complement(2248..2736)
                     /locus_tag="P5164_03515"
     CDS             complement(2248..2736)
                     /locus_tag="P5164_03515"
                     /inference="COORDINATES: protein motif:HMM:NF037816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42996.1"
                     /translation="MVVRLSHRLRPLVPLLLAVVALLAAASPADAARRRDLLRDEKRG
                     GHTLSRHVGKSDAELRERLLRDRRISAASTWTDRATAEEGVAAAIAANRSRIERWTSR
                     EGSRANLVLDWPGERGKVVGRSLRRGEKVPVPCTRAVVILRWSVDAEIYYVLTSYPEA
                     SR"
     gene            complement(2801..5227)
                     /locus_tag="P5164_03520"
     CDS             complement(2801..5227)
                     /locus_tag="P5164_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRY42997.1"
                     /translation="MSDNSCDDRWLWSFGAFRLDARARLLLRDGLPVPLTPKAFDVLV
                     HLARNENRAVAREELLEAVWPGTVVTDASLTQAVFLVRRALGEAEGTEMLETVPRFGY
                     RLNLPAASGGIVEEEPRKSSEPAPSTAAEETDVAGKPAPGPMPGLPHARARRPSAALL
                     GVLVAGALAAAAIASILLRSPARGGTDGSETGAEALLALQREIAVPPDAQAILGSLDG
                     TVVLSARTAYYLLPADGAHSAVRVPLEREEALAEPLGGGRILLVRKGRVVARHPTKAE
                     EADLGPLPPDAPPARPGRLRCSRSGRFLAVRGDETLEVFEREPGGWARRIRSSVPFVE
                     GEVLDVGERLVALAQGGGRTVRAFGLPEGGRLLEAPFAERKVSAVAVDDALGRVAVGG
                     PFDSVAVFTPGDRSSPRLLPRRGWTYGLAWIADAPTLLASGLDGITAFRQDAESAAIL
                     STASPGGPLCVDSDAILALVPRRQRLAVVSYSGISPTARVPAGGRPLWAAEHDAEGKS
                     VFAGGRDGELWSLDTASLAIRRSGAHTDGIPSMVRDGRLLATSSDDKTVALWSLPGPE
                     LKLRTRAHDFLVNDLTVVDGAGGAELVTASSDGTVRRWSWPGIEPVETIDVAGLLGRE
                     VSLHAIAPLPGARRILAGTWSSSLLELVARESGWSVREHPVPSKAVYRLAPVPRLGIW
                     VAVGIEPSSLHLFDLATGVLHPLDAAGLDAMWVVPVPGKDDVVVVGLDGLSRYSFSAP
                     LPDAAGRRTVTYRAWSGRRTGAGLQTATLLPDGSLWGGTVHGELLRFDAGRFGGSPIL
                     TRALDLGPPR"
     gene            5313..6584
                     /locus_tag="P5164_03525"
     CDS             5313..6584
                     /locus_tag="P5164_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42998.1"
                     /translation="MRRNRRTPPTFLGATLAGAALAVALPSAAANVLQNGSFETGLGG
                     WTATAPVSWEPDGSSGPGSGSVRLRGNPGVVGALSLSQCAAVTPGADYDFGATALLPY
                     SPESQGGVSLRVVWHSWPGCEGAALGLAPALDFPHVAPAVWTRRRLERLRAPSGAASA
                     SVLVVPWSSGPESAFSAFVDDVELSPSSRFVTLTVPTAASVDGANGERFQTTLVLTNA
                     ALYTRRVDVALRCASNVPCGTSTVSLQLAPRETRAFADLLLDVFGKRNGAGAIEVTYE
                     ATPGPVVVSARAATVHAERPGNGMALPVLPAAAARSTATFFGLTDGRAGVGGTRVNAG
                     VYNPSDATVFVGFSVHDESGDVRNTVYRWIEGREWAQIDDLFAVAGHGPARPGSYVTF
                     HSEAPVFPFLITIDNRSGDPTFLEPSESFQP"
     gene            6581..7834
                     /locus_tag="P5164_03530"
     CDS             6581..7834
                     /locus_tag="P5164_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY42999.1"
                     /translation="MRPLAGFLTSLAVLLAAAPAASAAGAENLVANGAFRDGISSWSR
                     LDLVSYSAMGAATPGSARITLPSRPEGGDAMLQCVAVEGWRLYDLGAAVRLPEWPEHS
                     GGVSVRLSWHALPNCQGPSLRGAPSLDFSYTEPAGWQVKEKRRIPAPQEAVSALVVVV
                     ARSAGDEPYPIFVDDVVLRRSVEEEDVFLPTAASVRGAKGERFETDLWVHNPAPAERM
                     FTLRLWRDGRPTTPVVLRVGAKQTRHLPDVLLSGLGQRDVAGAVQLSYDPGGGPMHFS
                     ARVVTVNPESPGNGTLLPVLRGDEARTDALFHGLSGARNDVAGAHRVNAGVFNPHDAP
                     VEAIFWLYDGDGTDLGTFSRTLPPGGWLQVNDVFREVGAAGVATEGARLAFHAALPVF
                     PFVIAVDNRSGDGTSIAARDVPVLP"
     gene            7939..8862
                     /locus_tag="P5164_03535"
     CDS             7939..8862
                     /locus_tag="P5164_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43000.1"
                     /translation="MPLLRSRWLRSLGFLVALAAGATGLRVALEPRETPAPGLKLSEG
                     ERGRIAALARASAADAGRRLADLSGVDVLLVGEEHYYVETQAWLTTLLDGVTDRRIAL
                     LLELPRGLQPEVDRWVATGRSEPLEEAIRAGDALPYGNLLAWAHRNRSRLSRVVAMDE
                     DGSRIFWNRVFLRDTRNRTMAAAVLSARRATPGDLVVAYGGQMHMLLAGRYRYDREDR
                     VPAGARLLGEGVARERIRSVLLSGEGKAPVCEAWAGPGALDMADAIGAEPWALFVDYP
                     IFRAVAGRELFDAFVSLGPLTRVSRREAGAR"
     gene            complement(8876..9208)
                     /locus_tag="P5164_03540"
     CDS             complement(8876..9208)
                     /locus_tag="P5164_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43001.1"
                     /translation="MYGSGSPLLAFLSSIARQLPELAVIVVGFVVVLGRKPAHPKASR
                     LAAAGLAVALATCVVGAAFYAAAPALVARVGALGAVYAVAGFAFSALDAAALGLLVAA
                     VVADRGPR"
     gene            9298..9543
                     /locus_tag="P5164_03545"
     CDS             9298..9543
                     /locus_tag="P5164_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43002.1"
                     /translation="MATPPPAERLLEILAGKIAEETGLDPALAREVAPSALAADSVDE
                     SQPIELRDARGHVVARVPFALVEEAFDDLDDEEDGER"
     gene            complement(9699..11039)
                     /gene="glnA"
                     /locus_tag="P5164_03550"
     CDS             complement(9699..11039)
                     /gene="glnA"
                     /locus_tag="P5164_03550"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HRY43003.1"
                     /translation="MATKAVEILKTCDKEGVRFLRLQFTDILGIIKNVEVPRSQFEKA
                     LDGEIQFDGSSIEGFTRIEESDMGLVPDLETFRIFPWSHTNGKVARLVCDIITPDGKA
                     FAGCPRQTLKRQREKAKDLGFSLMTGPEAEFFLFRRDAAGDPVVDTHDSGGYFDLTPV
                     DRGEDARRDIVVALEAMGFEVEAAHHEVAPGQHEIDFKYGDAVATADKVTTFKLVVKK
                     VAMDHGLHATFMPKPIFGVNGSGMHVHQSLMTGSGAGMKNAFFDPKAPYQLSKTAMYY
                     IGGVLKHAKAIAAVTNPLVNSYKRLVPGYEAPVNVAWSEKNRSPMVRIPARRGMGTRC
                     EVRMPDPACNPYLAFAVMLAAGLDGIKNRIDAGEPVNENIFEMSEREKRRRKIEQLPA
                     NLSDALDCLEKDDVVKNALGEHIHQHFVEAKRAEWATYISRVHPWEVEEYLQAY"
     gene            11213..11845
                     /locus_tag="P5164_03555"
     CDS             11213..11845
                     /locus_tag="P5164_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRY43004.1"
                     /translation="MTAPTRENAWALLTEFTASQALRRHALAVEASMRALARSSGVTD
                     EAEVERWGLVGLLHDFDYEKYPTEADHVFRGMEILRHRGWPEDVIRAVGGHAFYTGIP
                     RETPMEKAILAADELTGFVGACALVRPSKRIADVPVESVMKRLKEKAFAAKVDREYVR
                     RGAEEWGLPLPELVAVVLASQVPLADALGLGGTPASDLPDTPCPPPPPAP"
     gene            11972..13360
                     /locus_tag="P5164_03560"
     CDS             11972..13360
                     /locus_tag="P5164_03560"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRY43005.1"
                     /translation="MRALLIENALVAGMRSADDLHPRGAVWAEDGVIRALGSEARAAA
                     AASAARGEAVERVDAEGLWLFPGFVQTHVHLCQTLLRNGPDDLPLLPWLKTHVWPGEA
                     AHDEETLFLSARLGLAELAAGGTTAILDMGTVRHTDAVFRAAEAAGLRVTSGNALMDD
                     AAMNPPELFAETSEALEEGERLFARWNGAANGRLRFAWCPRFAVSCTDRILVEAASRA
                     KAQGVLVHTHASENLDEVALVRERSGRANIEHLREVGIGGRGTVLAHVIHTSEEERRL
                     LLADGTAVAHCPSSNLKLASGICPVPDYLARGIRVTLGADGAPCNDRLDAFSEMRHAA
                     LLQKIAAGPGALTAWQVVRMATAEGAEALGLGGVCGTIEAGKRADLVLLDPTAGFALP
                     TSWRDDPYGPLVFAFDRGNVVETRVDGEVVFHRERRGARALAPRAAEIEDAARRLRER
                     RSGNGLRHGGAS"
     gene            13414..13881
                     /locus_tag="P5164_03565"
     CDS             13414..13881
                     /locus_tag="P5164_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRY43006.1"
                     /translation="MAVRESAARLKSIVVGTDFSICAARALAHAVSLAQLSGARLHLV
                     HVLLEPVQALDVAAALPYPDVEVRKEWEEAARVKLDREVRAAEKRGVSTTAEIRWGRP
                     SDTIVDTAARQKASMIVLGTHGRSALEKLLLGSTAERVLRLSPVPVLTVREKR"
     gene            complement(13933..14760)
                     /locus_tag="P5164_03570"
     CDS             complement(13933..14760)
                     /locus_tag="P5164_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43007.1"
                     /translation="MERLNLVLTIPHGSVIATSLGVEGLAHHLSPGSGRHFHGRGIYV
                     DLALGDDGAAFSFLEEGGWRDTAGDTARAIQEVRSGKRTKTALSSNAFTITPIEAYRR
                     VLLVKTGGQALSMGPVSDVARFGPAPCRDSMAPAEVAQAIGAAEPARREPRLYAIFAP
                     LELLVLSNLTPVEYAWYATHRPGKVFRQVCFAELRTDQPQLAASSRYDNARKEIRENP
                     KKKTKTVAVQDLLNAVSFEEWVGFGDATAGGLYFGDRTRLTRTAFPVEIPRAWEKAD"
     gene            complement(14804..15382)
                     /locus_tag="P5164_03575"
     CDS             complement(14804..15382)
                     /locus_tag="P5164_03575"
                     /inference="COORDINATES: protein motif:HMM:NF013103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HRY43008.1"
                     /translation="MSIRATPVLPIEAIGEEFRSGTKSYEKPGPIDGVAIHRLARFVD
                     DRGFFQEIYRAKADHAGSEPLAAFFRDVPVAQLNYTIVNAENHVKGLHYHLKQQDVWF
                     CPHPSKAKFVLFDVRKDSPTYLRTQVVVAGDGQDLLVRIPEGVAHGYRPLTNPCALFY
                     LVTRTFDVNDPDEHRIPWDHPAVRELWEVPNG"
     gene            15431..>15963
                     /locus_tag="P5164_03580"
     CDS             15431..>15963
                     /locus_tag="P5164_03580"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY43009.1"
                     /translation="MPKRIIPVSSGKGGVGKTTVATNFALSLSRFAPTILVDLDTGTS
                     SVRNSIGVPVANDLYHFFRKGRRLDECVTSLPDSWDPDGLYRGFGFVAGPLHLIEEVT
                     NFGPEKKARLCDAINELPADYVVLDMKAGLDANVVDFLPWSNSGILVFTPHLPSATLA
                     ASDIVKAILFRKLRIVFS"
BASE COUNT         2158 a         5971 c         5606 g         2228 t
ORIGIN      
        1 gattccgacg agggcgaacc cgtcgactcc gccggcggcc tcgacgacct cccgggccat
       61 gcgggcgacc gcgcgcgcca tcgccgcggg atccatcagc cggagcttgc ggtaggtgcc
      121 gttcgcttcg cccatcgctc gaaccggcgg gacagtacac gggccgagcc gccccgtcaa
      181 cgaatgccgg ggttccttcg tcacggcgct ccggtccgga cggcgcggga ggcgacaatc
      241 ccgcccgtga acgtctcgga cttcgatttc gatctccccc cggaggcgat cgcccagcgt
      301 cccgccccgc gcggcacgtc gcgactcatg accctcgacc gcaccacggg cgcgacgggg
      361 caccggaacg tcgcggacct gccgggcctc ctcctccccg gcgacctcct cgtcgtgaac
      421 gacacgcgcg tcgtcccggc ccgcctcttc ggcctcgacg agacggggcg ccggacggag
      481 ttcctcctcg tcgagaggac cggggactcc tccgcgtggg actgcctcgc gaagccggga
      541 cggcgcgcga agccgggccg gcgcttcgac ttcggcgccg gcctcgtcgg gaccgtcgtc
      601 tcgaaggagg agtcgggcca ctaccgcgtc gccttctccg ggacgccgct cgccgaggcg
      661 ctcccgctcg tggggacgac gccgctcccc ccctacatcc accgcgaggg agggatggcg
      721 gacgcgcgcg acgccgtcga gtaccagacc gtctacgcgc gcgagcccgg cgcgatcgcc
      781 gccccgacgg ccgggctcca cctgacgccc tcgctcctcg acgcgctccg cgcccgcggc
      841 atcgaggtcg tctccgtgac gctgcacgtc gggatcggca cgttcaagcc ggtgaaggtc
      901 gagcgggtcg aggagcaccg gatggacccg gaacgcggcg tcatcccgga ggagacctcc
      961 gtctcaatcc ggcgcgcgaa ggaagagggg cgccgcgtcg tcgccgtcgg gacgacgagc
     1021 gtgaggacgc tcgaggccgc ggcgcgggag cacggcgggg tcgtcgcccc cggaccgttc
     1081 gagacgcgcc tcttcctcgt tccgggcgcg gagttccgaa tcgtcgacgc gctcctgacg
     1141 aacttccacc tcccccgctc gacgctcctc atgctcgtca gcgccttcgc cggacgggag
     1201 cgggtcctcg ccgcgtaccg ggaggcggtg cgctcgggct accggttctt ctcgtacggc
     1261 gacgcgatgc tcgtcgggtg agagtggatc ggggaggctc gcggcgcgtc agcgcggcga
     1321 gaaggcggcg cggactgcgg cttcttccgc cgacgtcatc cggggggcgc ccttccggag
     1381 caacccgacg aagtggtcga cctcctcctc cttcgaaggg aagccgacgt tcgtcccggg
     1441 gccggcgagg ttcgacgtcg cgatcggctt ccgcgtccgc gcgtcgagga cggccgtgaa
     1501 gggaagcccg gcgtcgctcc ccgcgaccga ctcgaggaac gtctccgcgc cggcgttctc
     1561 cgtctccttc cgctcgccgc gctcgaagac ggtcagctcg gcccggacga agtgccggcc
     1621 gacgacctcc gcgattcccg gcgcggcgag caccgcttcg agcttccggc accagccgca
     1681 ccacgaggcg tggaaaagga cgaggacgtt cttcttctcc gctcgggccg ccttcgccgc
     1741 ggccgcgagg accgcttcgg cggggggagg ggcgagggcc gcgccggccg ggggcgcggc
     1801 gacggccgcc ggcgacgcga gacggctcgt gacgatgagg gcggcggcga tgagacggcg
     1861 gcgcgacatg gccgcgaagt gtaagacgcg ggcggggccg gcgcccggcc tcgagacgga
     1921 gggcctactc gccggcgagg atccgaagca cccggtcggc ctcggcggcc gtcgacggcc
     1981 tccagccggt gccgaagccg gccccgagga gctttcgaag ctcccggacc tccatgccct
     2041 cgagggcggc gcgaagcttc ttcgcctccg gcgcgacgga gcgcttctcg tcgtccgccg
     2101 catcgcggcg gaaggcgcgc gcggcgtcct cggaatccgc gtactcggtc gtccagtcct
     2161 cgtgaaggta gccgcggagc gcgtcgcgca gcgcggggta ggcgcggacg tcgaccgtcg
     2221 cgggcgcctt cttcgtcgtc gtcgccgtca tcgggaagcc tccggatagg acgtcaggac
     2281 gtaatagatc tcggcgtcga cgctccatcg gaggatgacg acggcccgcg tgcacgggac
     2341 cggcaccttt tctccgcgtc ggagcgatct cccgaccacc tttccgcgtt cgccgggcca
     2401 gtcgaggacg aggttcgcgc gagagccttc gcggctcgtc cagcgctcga tccggctccg
     2461 gttggcggcg atcgccgcag ccacgccctc ctccgccgtc gcccggtcag tccaggtcga
     2521 ggccgcggag atccggcggt cccgcagcag gcgctcgcgc agctccgcgt cgctcttccc
     2581 gacgtgccgc gagagcgtgt gcccgccgcg cttctcgtca cggaggaggt cccgcctgcg
     2641 ggccgcgtcc gcgggagagg ccgcggcgag gagcgcgacg acggcgagga ggaggggaac
     2701 gagcgggcga aggcggtgcg agaggcgaac gaccacgcgc ggaggttagc tgaaacgacg
     2761 gcgtctccgc tcgccgatcg gggatttcgg ccgggcacgc tcagcgcggc gggccgaggt
     2821 cgagggcgcg ggtgaggatc ggagatccgc cgaagcgccc cgcgtcgaac cggaggagct
     2881 cgccgtgcac cgtgccgccc cacagcgagc cgtcgggcag gagcgtcgcg gtctggaggc
     2941 cggccccggt ccggcgcccg gaccaggccc ggtacgtcac cgtccggcgt cccgccgcgt
     3001 cgggcagcgg cgccgagaac gagtacctgg aaaggccgtc cagcccgacg acgacgacgt
     3061 cgtccttgcc cggcacgggc acgacccaca tcgcgtcgag gcccgccgcg tcgagcgggt
     3121 gcaggacgcc cgtggcgagg tcgaagaggt gcaggctcga cggctcgatc cccaccgcga
     3181 cccagatccc gagccggggt acgggagcga ggcgatagac cgccttcgaa ggaaccgggt
     3241 gctccctcac gctccagccg ctctcgcgcg cgacgagctc gaggaggctc gagctccagg
     3301 tccccgcgag gatccggcgc gccccgggca gcggcgcgat cgcgtggagc gaaacctcac
     3361 gtccgaggag ccccgcgacg tcgatcgtct ccaccggctc gattccgggc cagctccagc
     3421 gccgcacggt gccgtccgag gaggctgtga cgagctcggc ccctcccgcc ccgtcgacga
     3481 ccgtgaggtc gttcacgagg aagtcgtgag cccgggtccg gagcttcagc tccggaccgg
     3541 gaaggctcca gagcgcgacg gtcttgtcgt cggacgacgt ggcgaggagc cggccgtcgc
     3601 gcaccatcga ggggatgccg tccgtgtggg ctcccgatcg gcggatcgcg aggctcgccg
     3661 tgtcgaggga ccagagctca ccgtcccggc cgccggcgaa gacggacttc ccctcggcgt
     3721 cgtgctcggc cgcccagagc ggccggcctc ccgccggcac ccgagccgtc ggcgagattc
     3781 cggaatagga gacgacggcg aggcgctgcc gacgcggtac cagggcgaga atcgcatccg
     3841 agtcgacgca gagcggcccg cccggcgacg ccgtcgagag gatcgccgcg ctctccgcgt
     3901 cctgccggaa ggccgtgatc ccgtcgaggc cggaggcgag gagcgtcggc gcgtcggcga
     3961 tccaggcgag gccgtacgtc cacccgcggc gcggcaggag ccgcggggag gagcgatcgc
     4021 caggcgtgaa gacggccacg gagtcgaacg gcccgccgac ggcgacacgt ccgagtgcgt
     4081 cgtcgaccgc cacggccgag accttcctct ccgcgaacgg ggcctcgagg aggcggcccc
     4141 cctccggcag gccgaacgcg cgaaccgtcc gccctccccc ctgggccagg gcgacgagcc
     4201 gctcgccgac gtcgaggacc tctccctcca cgaagggcac gctcgagcga atcctcctcg
     4261 cccagccccc gggctcccgc tcgaagacct cgagcgtctc gtcgccgcgg accgcgagga
     4321 accgcccgga gcgcgagcag cggagcctgc cgggtcgtgc agggggcgcg tccggcggca
     4381 ggggaccgag gtccgcctcc tcggccttcg tcgggtgcct cgcgacgacg cgtcccttcc
     4441 tcaccaggag gatccgtccg ccgccgagcg gctccgcgag ggcctcctcc cgctcgagcg
     4501 gcacgcggac ggccgagtgc gcgccgtccg cgggcaggag gtagtaggcg gtccgcgccg
     4561 agaggaccac cgttccatcc agtgacccga ggatggcttg cgcgtcgggg gggacggcga
     4621 tctcccgctg aagggcgagg agcgcctcgg cccccgtctc ggagccgtcc gttcctccgc
     4681 gcgccgggct ccggaggagg atcgaggcga tcgcggctgc ggccagggcc ccggccacga
     4741 ggacgccgag gagggccgcc gagggccgcc gcgcacgagc gtgcgggagt ccgggcatcg
     4801 gcccgggagc cggcttcccg gcgacgtccg tttcctcggc ggccgtcgat ggggccggct
     4861 ccgaagactt ccgaggctcc tcctcgacga tcccgcccga cgcggcgggc aggttcagcc
     4921 ggtatccgaa cctcggaacg gtctccagca tttccgtccc ctccgcctcg ccgagcgctc
     4981 tccggacgag gaacacggcc tgcgtgaggg acgcatcggt gacgacggtt cccggccaca
     5041 cggcctcgag gagctcctcc ctcgcgaccg cgcgattctc gttccgggcg agatgcacga
     5101 ggacgtcgaa ggccttcggc gtgagcggaa ccggcaggcc gtcccggagg agaaggcgcg
     5161 cccgcgcatc caggcgaaac gcgccgaaac tccataacca cctgtcatcg cacgagttat
     5221 ccgacatcac agaatcctgt gaggtgatcc tagccgaaaa ttgatccgcc tccgaggaac
     5281 tcgtgggaac agcgaccgta ccttcggtcg ccatgagacg aaaccgcaga acgccgccga
     5341 ccttcctcgg cgcgaccctc gcaggagcgg ccctcgccgt cgctctcccg tcggccgccg
     5401 ccaacgtcct tcagaacggc tccttcgaga cagggctcgg cggctggacg gcgacagccc
     5461 ccgtgagctg ggaaccggac ggatcctcgg gccccggctc gggttccgtc cgcctccgcg
     5521 ggaaccccgg cgtcgtcggc gcgctctcgc tctcccagtg cgccgccgtc acgcccggcg
     5581 ccgactacga cttcggcgcg acggcgctcc tcccgtactc cccggagtcc cagggcggcg
     5641 tatctctgcg cgtcgtctgg cactcctggc ccggctgcga gggggcggcc ctgggcctcg
     5701 ctccggccct cgacttccct cacgtcgccc cggccgtctg gacccgccga aggctcgaga
     5761 ggcttcgcgc cccgtccggc gccgcgagcg cctcggtcct cgtcgtcccc tggtcctccg
     5821 gcccggagtc cgccttctcc gcgttcgtgg acgacgtgga gctctccccc tcctcgaggt
     5881 tcgtcacgct gacggttccg accgcggcgt cggtcgacgg agcgaacggt gagcgcttcc
     5941 agaccacgct cgtcctgacg aacgccgcgc tctatacccg gcgcgtcgac gtcgcgcttc
     6001 gctgcgcgtc gaacgttccg tgcggaacct cgacggtgag cctccagctg gcgccgcggg
     6061 agacgcgcgc cttcgcggac ctcctcctcg acgtcttcgg aaagcggaac ggcgccggcg
     6121 cgatcgaggt gacctacgag gcgacgcccg gcccggtcgt cgtgtcggcg cgggcggcga
     6181 ccgtccacgc ggagcggccc ggcaacggca tggccctgcc ggtccttccg gccgcggcgg
     6241 cccggagcac ggcgaccttc ttcggcctga ccgacggccg agccggggtc ggagggacgc
     6301 gcgtgaacgc gggcgtctac aacccgtctg acgccaccgt cttcgtcggt ttctcggtcc
     6361 acgacgagag cggggacgtg aggaacacgg tctaccggtg gatcgaaggc cgggagtggg
     6421 cacagatcga cgacctcttc gccgtggcag ggcacggacc ggcccgtccg ggctcctacg
     6481 tgacgttcca ttccgaggct cccgtcttcc cgttcctcat caccatcgac aaccgctcgg
     6541 gcgaccccac gttcctcgag ccctcggaga gcttccagcc atgagacctc tcgccggatt
     6601 cctcaccagc ctcgccgtcc tcctcgccgc cgcccccgcg gcgtccgccg cgggagcaga
     6661 gaacctcgtc gcgaacggcg cgttccgcga cgggatctcc tcctggtcgc gcctcgacct
     6721 cgtgtcgtac agcgcgatgg gggccgcgac gccgggctcg gctcgcatca cccttcccag
     6781 ccgccccgag gggggcgacg cgatgctcca atgcgtcgcg gtcgagggct ggcggctcta
     6841 cgacctcggg gccgccgttc gtctccccga gtggccggag cactccggag gcgtctccgt
     6901 ccgtctcagc tggcacgccc tcccgaactg ccaggggccg tcgctgcgcg gcgccccgtc
     6961 tctcgacttc tcctacaccg agcccgccgg gtggcaggtg aaggagaagc ggcggatccc
     7021 ggcgccgcag gaggccgtca gcgcgctcgt cgtcgtcgtg gcccgctccg cgggagacga
     7081 gccctacccg atcttcgtcg acgacgtcgt cctccggcgc tccgtcgagg aggaggacgt
     7141 cttcctcccg acggctgcct ccgtccgcgg cgcgaagggc gaacggttcg agaccgacct
     7201 ctgggtccac aaccccgccc ccgccgagcg gatgttcacg ctgcggctct ggcgggacgg
     7261 ccggccgacg acgcccgtcg tcctgcgtgt cggcgctaag cagacccggc accttcccga
     7321 cgtcctcctg agcggcctcg gccagcgcga cgtcgccggc gccgtccagc tctcgtacga
     7381 cccgggcgga ggtccgatgc acttctcggc tcgcgtcgtg acggtgaacc cggagagccc
     7441 gggcaacggg acgctcctcc ccgtccttcg cggggacgag gcccgcacgg acgccctgtt
     7501 ccacggcctc tcgggcgcgc gaaacgacgt cgcgggcgcg caccgcgtca acgcgggcgt
     7561 cttcaacccc cacgacgctc ccgtcgaggc gatcttctgg ctctacgacg gcgacggcac
     7621 cgacctcggc accttctcgc ggacgctccc gccgggcggc tggctccagg tgaacgacgt
     7681 cttccgcgag gtcggcgccg cgggcgtcgc gactgaaggg gcgaggctcg cgttccacgc
     7741 ggcgctcccc gtcttcccgt tcgtcatcgc ggtcgacaac cgctcgggcg acggcacctc
     7801 catcgcggcg cgcgacgtcc cggtccttcc ctgacctccg ccgtgccgcc gggagtccga
     7861 tcctcccggc ggcccgctcc cgggcagccg cggcggccct ccaggaaccg cccggcggtc
     7921 ccgtccgttt ctaccgccat gccgctgctc cgctcgcgct ggctccgcag cctcggcttc
     7981 ctcgtcgccc tcgccgccgg cgcgaccggc ctccgggtcg ccctcgagcc ccgggagacg
     8041 ccggctcccg ggctgaagct ctcggagggg gagcgcggcc gtatcgcggc tctcgcccgc
     8101 gcctcggcgg ccgatgccgg gcggcgcctc gcggacctct ccggggtcga cgtcctcctc
     8161 gtcggcgagg agcactacta cgtcgagacg caggcctggc tcacgacgct cctggacggc
     8221 gtgaccgacc gcaggatcgc gctcctcctc gagctccccc gcggcctcca gcccgaggtg
     8281 gaccggtggg tcgcgacggg ccgctccgag cccctcgagg aggcgatccg cgcgggggac
     8341 gccctcccct acggcaacct cctcgcctgg gcgcatcgga accgctcgcg cctctcccgc
     8401 gtcgtggcga tggacgagga cgggagccgc atcttctgga accgggtctt cctgcgcgac
     8461 acgcggaacc ggacgatggc cgccgcggtc ctctcggccc gccgcgccac gccgggagac
     8521 ctcgtcgtcg cctacggcgg gcagatgcac atgctgctcg cgggccgcta ccgctacgac
     8581 cgggaagacc gcgtcccggc gggagcccgg ctcctcggcg aaggagtcgc tcgcgagcgg
     8641 atccggagcg tcctcctctc gggcgaaggg aaggcgcccg tctgcgaggc ctgggccgga
     8701 cccggcgcac tcgacatggc cgacgcgatc ggcgccgagc cctgggccct cttcgtcgac
     8761 tacccgatct tccgggcggt cgccgggcgc gagctcttcg acgcgttcgt gagcctcggg
     8821 ccgctgacac gcgtctcccg gcgcgaggcc ggggcccgtt gagggccgcg gcccctcagc
     8881 ggggcccgcg gtccgcgacg acggcggcga cgaggaggcc gagggcagcg gcgtcgagcg
     8941 ccgagaaggc gaagccggcg accgcgtaga ccgcaccgag cgcgccgaca cgcgcgacga
     9001 gggccggcgc ggccgcgtag aaggccgcgc cgacgacgca cgtggcgagc gccacggcga
     9061 gcccggcggc cgcgagtctc gaagccttcg ggtgcgccgg cttccggccc aggacgacga
     9121 cgaagccgac gacgatcacg gcgagctcgg ggagctgccg cgcgatcgac gagaggaacg
     9181 ccaggagggg cgacccgctt ccgtacatga cgcggacggt agcagagccg gcgtgcggcc
     9241 ggccgccggc gcggcgggaa cgttgacaga ctcgggggcg cgaccgatcg tcgagggatg
     9301 gccaccccgc ctcccgccga acgcctcctc gagatcctcg ccgggaagat cgccgaggag
     9361 acggggctcg acccggccct cgcgcgcgag gtcgcgccgt ccgccctcgc cgccgattcc
     9421 gtcgacgaga gccagccgat cgagctccgg gacgcgcgcg gccacgtcgt cgcgcgcgtc
     9481 cccttcgctc tcgtcgagga ggccttcgac gacctcgacg acgaggagga cggcgagcgc
     9541 tgagggggcc cgccggcggt cctccgcccc ggcagcgggg cgaacgggaa acccggcgac
     9601 ccgagttccc cttcgcctcg tgctcgcgaa gcgcgccgcc tcaccgcgcg gaagggggcc
     9661 cgggggaaac ccggcggtcc gggtttcccc cgatcacatc agtaggcctg caggtactcc
     9721 tccacctccc acgggtggac gcgggagatg taggtcgccc actcggcccg cttggcctcg
     9781 acgaagtgct ggtggatgtg ctcgccgagg gcgttcttga cgacgtcgtc cttctcgagg
     9841 cagtcgaggg cgtcggagag gttcgccggg agctgctcga tcttccgacg ccgcttctcc
     9901 ctctcggaca tctcgaagat gttctcgttc accggctcgc ccgcgtcgat ccggttcttg
     9961 atgccgtcga gaccggcggc gagcatcacc gcgaacgcga ggtacgggtt gcaggcaggg
    10021 tcgggcatcc gaacctcgca gcgggtcccc atgccgcgac gcgcggggat gcggaccatc
    10081 ggcgaacggt tcttctccga ccaggcgacg ttcaccggcg cctcgtagcc cggcacgagc
    10141 cgcttgtagg agttcacgag cgggttcgtc acggccgcga tcgccttcgc gtgcttcagg
    10201 acgccgccga tgtagtacat ggcggtcttc gagagctggt acggcgcctt cgggtcgaag
    10261 aaggcgttct tcatcccggc gcccgagccg gtcatcagcg actggtggac gtgcatcccc
    10321 gagccgttga cgccgaagat cggcttcggc atgaacgtcg cgtggaggcc gtggtccatg
    10381 gcgaccttct tcacgacgag cttgaacgtc gtcaccttgt ccgcggtcgc cacggcgtcg
    10441 ccgtacttga aatcgatctc gtgctggccg ggagccacct cgtggtgcgc cgcctccacc
    10501 tcgaagccca tcgcctcgag cgcgacgacg atgtcccgtc gcgcgtcctc gccccggtcg
    10561 acgggcgtca ggtcgaagta gccgcccgag tcgtgggtgt cgacgaccgg gtctcccgcg
    10621 gcgtcccggc gaaacaggaa gaactccgct tcgggccccg tcatgagcga gaagccgaga
    10681 tccttcgcct tctcccgctg ccgcttcagc gtctgacggg ggcacccggc gaaagccttt
    10741 ccgtccgggg tgatgatgtc gcagacgagg cgcgcgacct tcccgttcgt gtgcgaccag
    10801 gggaagatcc ggaacgtctc gaggtcgggg acgagcccca tgtccgactc ctcgatccgg
    10861 gtgaagccct cgatcgagga gccgtcgaac tggatctccc cgtcgagcgc cttctcgaac
    10921 tgggacctcg ggacctccac gttcttgatg atcccgagga tgtcggtgaa ctggaggcgc
    10981 aggaaccgca ccccctcctt gtcgcacgtc ttcaggatct cgaccgcctt cgtcgccatc
    11041 gcttctccct ttctcctcgg ttcccgctcc aggatggcgc ggaatttccg ccaccttgcg
    11101 ggattcgtca agggaaatct ccagaatgac gagatgattt cgccaaaaac atcaaaatgg
    11161 atatctcgct ctgtttaaat acgggcatcg cggacgcggg atactccgct ccatgaccgc
    11221 accgacccgc gagaacgcct gggccctcct gaccgagttc accgcctcgc aggcgctccg
    11281 ccgccacgcc ctcgccgtgg aggcgtcgat gcgcgccctc gcccgctcct ccggcgtcac
    11341 ggacgaggcc gaggtcgagc gctgggggct cgtcggcctg ctgcacgact tcgactacga
    11401 gaagtacccc accgaggcgg accacgtctt ccgcgggatg gagatcctcc gccaccgggg
    11461 atggccggag gacgtgatcc gggcggtcgg cggccacgct ttctacacgg ggatcccgcg
    11521 cgagacgccg atggagaagg cgatcctcgc cgccgacgag ctgaccggat tcgtcggggc
    11581 ctgcgcgctc gtgagaccgt cgaagaggat cgccgacgtc ccggtcgagt cggtgatgaa
    11641 gcgtctgaag gagaaggcgt tcgccgcgaa ggtcgatcgc gagtacgtga ggcgcggcgc
    11701 cgaggaatgg gggctcccgc tgcccgagct cgtcgcggtc gtcctcgctt cccaggtgcc
    11761 tctcgccgat gcgctcggcc tcggcgggac gccggcgtcc gacctccccg acaccccctg
    11821 cccccctccg ccgccggcgc cctgagcggg acccggctcg gaggccgcgc ggtcgccgtc
    11881 gcgaccccgc gccgcggtca ccgggacggc cgcgggctcc cgacggcctc tccgctcgac
    11941 gcgagccgcg cgcgggccga tactccgccg gatgcgcgct cttctgatcg agaacgccct
    12001 cgtcgccggg atgcgctcgg ccgacgacct ccacccgcgc ggcgcggtat gggcggagga
    12061 cggcgtcatc cgcgccctcg gctccgaggc ccgcgccgcc gcagcggcct cagcggcccg
    12121 cggcgaggcg gtggagaggg tcgacgccga ggggctctgg ctcttccccg gcttcgtcca
    12181 gactcacgtc cacctctgcc agactctcct ccggaacggc cccgacgacc tccccctcct
    12241 cccctggctg aagacgcacg tctggccggg cgaggccgcc cacgacgagg agaccctctt
    12301 cctctccgcc cggctcgggc tcgccgagct cgcggcaggc gggacgaccg cgatcctcga
    12361 catggggacg gtccggcaca ccgacgccgt cttccgggcc gccgaggccg cgggcctccg
    12421 cgtcacgagc ggcaacgccc tcatggacga cgccgcgatg aacccgcccg agctcttcgc
    12481 cgagacgtcc gaggcgctcg aggagggaga gcggctcttc gcccgctgga acggcgcggc
    12541 gaacggacgg ctccgcttcg cctggtgccc ccgcttcgcc gtctcgtgca cggaccggat
    12601 cctcgtggag gcggcctccc gggcgaaggc gcagggcgtc ctcgtccaca cccacgcctc
    12661 cgagaacctc gacgaggtgg cgctcgtgcg cgagcggagc ggccgcgcga acatcgagca
    12721 cctgcgcgag gtggggatcg gcggccgcgg gacggtcctc gcccacgtca tccacacgag
    12781 cgaggaggag cgccgcctcc tcctcgccga cggcacggcc gtcgcgcact gcccctcctc
    12841 gaacctgaag ctcgcctccg ggatctgccc tgtccccgac tacctcgcgc gcggcatccg
    12901 cgtcacgctc ggcgccgacg gggcgccgtg caacgaccgg ctcgacgcct tctccgagat
    12961 gcggcacgcg gcgctccttc agaagatcgc cgcggggccg ggcgcgctga ccgcctggca
    13021 ggtcgtccgg atggcgacgg ccgaaggggc cgaagcgctc gggctcggcg gcgtctgcgg
    13081 gacgatcgag gccggcaagc gggccgacct cgtcctcctc gacccgacgg ccgggttcgc
    13141 ccttccgacc tcctggcgcg acgaccccta cggcccgctc gtcttcgcgt tcgaccgggg
    13201 gaacgtcgtc gagacgcgtg tcgacggcga ggtcgtcttc caccgcgagc ggcgcggggc
    13261 ccgggcgctc gcgccgaggg ccgccgagat cgaggatgcc gcccgcaggc ttcgcgagcg
    13321 tcgctcgggg aacgggctcc ggcacggcgg cgcctcttga agagagcgcc gctggggccc
    13381 agactcgggc cggcagaccc ctggaggtga acgatggccg tacgtgaaag cgccgctcgc
    13441 ctgaagagca tcgtcgtcgg caccgacttc tcgatctgcg ccgcccgcgc cctcgctcac
    13501 gccgtctcgc tcgcgcagct ctcgggcgcc cggctgcacc tcgtccacgt cctgctcgag
    13561 ccggtccagg cgctcgacgt cgcggccgcg cttccctacc ccgacgtcga ggtccggaag
    13621 gagtgggagg aggccgcgcg cgtcaagctc gaccgcgagg tgagagcggc cgagaaacgc
    13681 ggggtctcga cgacggcgga gatccgctgg ggccgcccgt ccgacacgat cgtcgacacc
    13741 gcggcacgcc agaaggcgtc gatgatcgtc ctcgggaccc atggccggtc ggccctcgag
    13801 aagctcctcc tcggctcgac cgccgagagg gtcctccggc tctcgcccgt ccccgtcctg
    13861 accgtccgag agaagaggtg accgcgcgcc ggggccccgc cccggcgcgc ccgtcccgca
    13921 ggctggttcg gcctagtcgg ccttctccca ggcgcggggg atctcgacgg ggaacgccgt
    13981 ccgcgtaagc cgcgtgcggt cgccgaagta gagccccccg gcggtcgcgt ctccgaagcc
    14041 gacccactcc tcgaaggaga cggcgttcag gaggtcctgg acggccaccg tcttcgtctt
    14101 cttcttcggg ttctcgcgga tctccttgcg ggcgttgtcg taccggctcg aggcggccag
    14161 ctgaggctgg tcggtccgga gctcggcgaa gcagacctgc cggaagacct tgcccggccg
    14221 gtgcgtggcg taccaggcgt actccaccgg cgtcaggttc gagaggacga ggagctcgag
    14281 cggcgcgaag atggcgtaga gccgcggctc gcgccgggcc ggctcggccg cgccgatcgc
    14341 ctgcgcgacc tcggccggag ccatgctgtc gcggcacggg gcgggaccga accgggcgac
    14401 gtcggagacc ggccccatcg agagcgcctg gccgcccgtc ttcacgagca ggacccggcg
    14461 gtaggcctcg atcggggtga tcgtgaaggc gttcgaggag agcgccgtct tcgtcctctt
    14521 gccggagcgg acctcctgga tcgcgcgggc ggtgtctccg gccgtgtcgc gccagccccc
    14581 ttcctcgagg aaggagaacg ccgcgccgtc gtctcccagc gccaggtcga cgtagatccc
    14641 ccgcccgtgg aagtgccggc ccgaacccgg ggagaggtgg tgggcgagcc cctccacgcc
    14701 gaggctcgtc gcgatgacgg agccatgggg gatcgtcagg acgaggttca accgttccat
    14761 cgtgtctcct gcctcccgga cgcgccgggg cgcgaaaggt acatcagccg ttcggaactt
    14821 cccagagctc ccggacggcc gggtggtccc aggggatccg gtgctcgtcc gggtcgttca
    14881 cgtcgaacgt ccgggtgacg aggtagaaga gggcgcacgg gttcgtcagc ggtcggtagc
    14941 cgtgggccac cccttccggg atcctcacga ggaggtcctg gccgtcgccc gccacgacca
    15001 cctgggtccg aaggtaggtc ggagagtcct tccggacgtc gaagaggacg aacttcgcct
    15061 tcgaggggtg gggacagaac cagacgtcct gctgcttcag gtggtaatgg agccccttga
    15121 cgtggttctc cgcgttcacg atcgtgtagt tgagctgcgc gaccggaacg tcccgaaaga
    15181 aagccgcgag gggctcgctt ccggcgtggt ccgccttcgc ccggtagatc tcctggaaga
    15241 acccgcggtc gtcgacgaaa cgggcgaggc ggtggatcgc gacgccgtcg atcggcccgg
    15301 gcttctcgta gctcttcgtc ccggaccgga actcctcccc gatcgcctcg atcggaagga
    15361 cgggcgtggc gcggatgctc attccgcgat tctaatcggc gcgggaggcg tgctagcctc
    15421 ccggagcccg atgccgaaga ggatcattcc cgtctcctcc gggaagggcg gcgtcggaaa
    15481 gacgaccgtc gcgaccaact tcgcgctatc gctctcccgg ttcgctccga cgatcctcgt
    15541 cgacctcgac accggcacct cctcggtgag gaactcgatc ggcgtcccgg tcgcgaacga
    15601 cctctaccac ttcttccgga aggggcgccg cctcgacgag tgcgtgacct ctctccccga
    15661 ctcgtgggac cccgacggcc tctaccgcgg cttcgggttc gtggcggggc cgctgcacct
    15721 gatcgaggag gtgacgaact tcggcccgga gaagaaggcc cggctctgcg acgcgatcaa
    15781 cgagctcccg gccgactacg tcgtcctcga catgaaggcg gggctcgacg cgaacgtcgt
    15841 cgacttcctc ccctggtcga actccgggat cctcgtcttc acgccccacc tcccttcggc
    15901 gacgctcgcc gcctccgaca tcgtcaaggc gatcctcttc cggaagctcc ggatcgtctt
    15961 ctc
//
LOCUS       DAOTEZ010000042        15906 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2502_length_15906_cov_5.557820, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000042 DAOTEZ010000000
VERSION     DAOTEZ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15906
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2502_length_15906_cov_5.557820"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1793
                     /locus_tag="P5164_03585"
     CDS             <1..1793
                     /locus_tag="P5164_03585"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRY43010.1"
                     /translation="FVREGLSGAPKLLLDPNTLDTKGLVTVSWTSPDPAGKLLAFGMY
                     RAGDENSTLYVLDVDSGRWLADEIPGKVGGVDWLPDSSGFFYRNLENVKDPYSGQVMF
                     HRLGSHPRQDARLFRQYTKEENAKLATTYGPGFSASEDGRWGLLSYATGTSSNDLWAV
                     DLDRYFRTGEFVKTDVILGSEGSSRGPILGDTLFLHTTDGAKNGRVVAVDLNAPARAG
                     WKTLVPERKDVSIRGVSLARGILAVTLLKDAATRIELYSLDGRPLGELPLPGIGTAFL
                     STSQDRTEAFLTYTSFNEPSGVYRVDLATKKVELWERPKVPVDPSIVEVKQVFYPSKD
                     GTKVPMFVVHKKGLKLDGGNPTLLYAYGGFNVSQTPSFSATLFPWFEAGGVYALAGLR
                     GGGEYGDAWHEAGMLERKQNVYDDFIAAAEWLVAQKYTKPSKLAVSGGSNGGLLTGAV
                     LTQRPELFGAVISAVPLLDMLRYQSFLMARYWIPEYGTAENPEQFRFLRAYSPYHNVK
                     KGTSYPAVLLTAGENDSRVHPLHARKMAALLQASTASDPATKPILLWVDREAGHGQGK
                     PLALRVRDVADQRMFLMWQLGMLPKESPRK"
     assembly_gap    1980..2079
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2172..2504)
                     /locus_tag="P5164_03590"
     CDS             complement(2172..2504)
                     /locus_tag="P5164_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43011.1"
                     /translation="MFVRSQVRIPFVLVVAATALAAVASAQQLPAGAEKKMVLEEQDS
                     MASVLKRLEGKPVRLRMAGSGEEVLGKLQKVGRELAHLSDLSGREFFDAVVRIDQVAA
                     VSVQVRGR"
     gene            3076..3603
                     /locus_tag="P5164_03595"
     CDS             3076..3603
                     /locus_tag="P5164_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43012.1"
                     /translation="MREAVRSASRSALLAALVAPLLVVSPRASSADGRGPLVEPRLGP
                     LVHRTVERAASRLAVPGCAMLLDDFADVRSGEPLSRALAASGRGPSDVFASLRFVDAD
                     HMVQCRRRATWAWVPVGADVVFVCRNRFTDLVRKDERLAGNVLVHEALHTLGLGENPP
                     SSEEITAAVVRRCGR"
     gene            complement(3688..4329)
                     /locus_tag="P5164_03600"
     CDS             complement(3688..4329)
                     /locus_tag="P5164_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRY43013.1"
                     /translation="MRASFADVTFDGDARRVSRAGAPVHLTPKAFALLGLLIEKRPAA
                     VSRLAIQEKLWPGVFVTEGNIDSLVKEIRKALGDGHGPNALIRTVHGFGFAFDAPVRE
                     EAEPPSARSHLLVWGAQTFPLAEGPNVLGRVREANVWLGHDSVSRAHARVFVAGDRAE
                     IVDLGSRNGTRVGERPVREAVVLRDGDEIVLGCVRVVYRFFAASPSMPTRSAG"
     gene            complement(4333..4980)
                     /locus_tag="P5164_03605"
     CDS             complement(4333..4980)
                     /locus_tag="P5164_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRY43014.1"
                     /translation="MGGAIAGEDLGPRLRFGGFSFDGGRRLLHRGGTALHLSPKAFQL
                     LTLLLERRPGAVAREEIFEALWPAEGVAAGRLTSLVSEVRHVLGSGPRGRTFIRTVHR
                     YGYAFDGDAHEMPVAPRHVLVRGLQHVELSQGGNLLGRERSATVRLGHPSVAHEHARI
                     VVTDDRAELEDLAATGTTFRGTEAIRGRVVLEDGDEIRVGAIRLTYRILAAVPGG"
     gene            complement(5105..5743)
                     /locus_tag="P5164_03610"
     CDS             complement(5105..5743)
                     /locus_tag="P5164_03610"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRY43015.1"
                     /translation="MRLRFGELTFEGGRRLLLRGPDVVHVSPKGFQLLELLLARRPNA
                     VSKAEIQEALWPRTFVSETNLPALVNEVRNALGDHAKEAEYVRTVHGFGYAFEAAVRE
                     LPAEAAGEFRHVVLWGSQEIALAEGSNVVGRERIADVWIGHPSVSREHARITVEGPVA
                     TIEDLGSRNGTLRGAEVVRGRVTLVDGDEIRLGSVVLVYRAGSTEISTQSAI"
     gene            6032..8719
                     /locus_tag="P5164_03615"
     CDS             6032..8719
                     /locus_tag="P5164_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRY43016.1"
                     /translation="MPLAAGQELGPYVVLAPVATGGMGLVYRARDRKLDREVALKVLP
                     DGLASHPKALALFESEAKSVAALSHPGIVAIHDFGRIGGTTFAVTELLAGETLRAAVT
                     RGPIPPPRAVDIAAQVADALAAAHARGLVHRDLKPENIFLTTCGRVKILDFGLAVRAA
                     PLSAGAGDAEVPTAPLVESSTGAPGTPAYMSPEQARGLAVDACSDQFALGAVLYEMLS
                     GLRPFQGSSHAEVLSSILRDEPAPLAERVPRLPDAVRRIVERCLRKRPEERYASTLDL
                     ARDLADCASRYREGSFTPTPKSSPVLPPPAFWAAAAATAVLLLAAAFAGRRAAVGGPA
                     ATYQSLLAGRQFVRLTDRSQDATTPAISPDGHLAAFVRRLSATNTDIYVKDLTGGALV
                     DVTGDHPGEDREPAFSPDGSLLAFRSDRDGGGLFVVETQGGAPRRLAPSGHDPAFFPD
                     GRLLVFATTATGTPYARLGARSALRTVDTATGVTRLLYEGDAMQPAVSPNGLRVAFWM
                     LREGGSQRDLATVAARFGGPSAPPVLVTDDPACDWSPFWSGDGRHLWFASDRGGTMNL
                     WRVRIDEESGEPLGPPEPLTVPARSAGPFQGSADGHAVVYQAGTSGYDLLRVPVDARG
                     FPSGPARNLLTDHEGVGELRISPSGDLLAFSTWRAGEDLWVVRTDGQGLRRITSSSFH
                     DRAPSFTADGRSLIFHSDRSGKFEIWKVSLDGGGLAPLTRLDGLHPFRALASPDGRLL
                     AVNNSRTGALLLPSDAPPGASWPAPLPPPEPGLRFELTSFSPDGRFAAGHAISGDERY
                     AGVFLHDLGSGRYERLTAAGSSPQFVLGGTRLYFLRQTSLFAPSTLAFLDLGTRRIHD
                     LQPEGTGSSIVSFTAAPDGSALYLVRERSRNDIWLMRMP"
     gene            complement(8728..10803)
                     /locus_tag="P5164_03620"
     CDS             complement(8728..10803)
                     /locus_tag="P5164_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRY43017.1"
                     /translation="MSVDTLRADRLPLYGYGGVATPALASLRADGVLFETAWAQAPLT
                     LPSHAALMTGTDAREHGVLDNSGYALAPSVPTIAEIAKANGYATGGAVSSVVLDGRSG
                     IARGFDFWDDDLGDAAPGEPMTQLERPGAATERALSRWIAGQGHAPFLAFLHLFEPHS
                     PYAPPEPFRSRWTDPYDGEIAAADAAVGSFVAGLKAAGLYDGSLVVFLSDHGEGLGDH
                     GEKEHGIFLYRESIRVPVVVKLPGNLFGGTTVGTPVGLTDVFRTVCSALGLKGCPDRP
                     GLVSLAELASGREGPRRRILSETFYPRTRFGWSPLASLADERWHFVDAPRPELYDLLE
                     DPSERTNRAAGKDGPIRSMRAELETRRSGFREPSPVDAEKARQLASLGYVTARSGVRD
                     DALPDPKDRIATLAPLRDGMAALRAGRPADAVRVLAPFLDRNPRVRDAWEVYAQALLG
                     EGRNGEALEAAERMLPLSAPGASEVLVSVASVALSAGRPEVALRHAEAARACNDPAAD
                     DVLARAHLAAGNLGGAEEAAKRVLARGRSPARALLVLARVATLRGDLPGALKLANRAR
                     DVSGEEARKLAGLHFVRGDVLARLGRAGEAEEELLTEIGLFPVRVEARAALVALRAAT
                     GRREGALAAAREIAANVPGPGGVAAAIRSLEAIGERAAARELRAGAARRLREEPGPRG
                     KGEAGLSGE"
     gene            complement(11012..13939)
                     /locus_tag="P5164_03625"
     CDS             complement(11012..13939)
                     /locus_tag="P5164_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRY43018.1"
                     /translation="MRLRRFRLLLLVLPIFLTASLLAQGLPTGTISGKAINEGQGLPG
                     VVVTAKSPSLQGARTAITSVNGDYALPNLAPGDYTLTFTMTSFQTVTRNLKVNASQSA
                     IVNVNMQLSAVATEVTVVSTSESISQTTQAATTISSDLTKKLPVTRTLLSAVALSPGV
                     NGNGPGGATTISGAESYDNLVTINGVVVQDNIRGSPRDLFIEDAIAETTTSTAAISAE
                     YGRFTGGVVNAITKSGGNTFSGSARATLNNDSWQATRPLQTVDYTDKTIPTYEATLGG
                     PIWKDRIWFFAAGRSRDTETTGQTYLTNVGYPSKTEELRLEGKLTVTPFQNHTFTASY
                     IDIANDATNVNPGYSPMELKTLYNPSYPQSLLALNYNGVLTDQVFVEAQYSQRKFAFE
                     NAGGYSRDLIAGTNMFDGTLGGLYNASPFCGVCEPETRDNENVLAKGTWFLSTKSLGS
                     HNVVFGYDNFNNKVYSNNYQSGSNYTVYSSATIVQGQNVYPVLGNDSVLVYWPIFELS
                     KGSNVRTHSIYVNDTWRMNDRFSFNIGLRWDKNDGKDSRGVTTSDDSAFSPRLAATWD
                     VKGDGRLRVGASYARYVGGQQENFIGGASSAGSPEIWVWYYEGEPINEGDPSSPVTTA
                     ATLERMFSWFGITGINQFPTRPGISPIQATVAGVSTQIRESLKSTYTDEYALSLAGAI
                     GTRGNFRLDGIYRRFGDFIDTFTDTTTGKVTDSVGITYDLALLQNTNAIERTYAALQV
                     QAAYRLFESLNIGGNYTYSHTYGNAVTEDRGSGPRSFGWTSYPEYFDPAWNIPTGSLP
                     QDQTHRLVAYATYDIPLPKVLGALNLSAVHTWNSGTPYGAVGSVASWPYVADVGYETP
                     PSSVNYYFTKRDAFRLPDTHRTDLALNYSYNIGPVEIFVQPQVTNVFGNEAVIAVNTT
                     IETRVNRRNDYVAFNPFTAQPTQGARGTGANWNYGPSFGKPTSSASYQAPRLFRIGFG
                     VRF"
     gene            14669..15193
                     /locus_tag="P5164_03630"
     CDS             14669..15193
                     /locus_tag="P5164_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43019.1"
                     /translation="MSEAVRGVSRSSLGAALLVALLPWTTTGADEPLRPEVDPRLGGY
                     VAQVVSRASRRFAEPGCVSLLVRFEDVRTGRPLSETLRTSGETPASHFAKLRFCSGDE
                     RLQCRGRETWAFTGIGSDSVFVCRSRFTALAKKSEKAAANILVHEALHTLGLGENPPT
                     SREITAAVHDHCGR"
     gene            15253..15723
                     /locus_tag="P5164_03635"
     CDS             15253..15723
                     /locus_tag="P5164_03635"
                     /inference="COORDINATES: protein motif:HMM:NF022338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRY43020.1"
                     /translation="METVYTLVPPPGKTSTALWIPVVLVLLISLVGIFFVYKSLSGAR
                     GSTFTLSPAGLVIRGDLWGRTIPTAQLRGALARVVDLRAETALAPRRRTAGTGLPGYR
                     SGWWKLENGEKALLYVTTTDRVVHVPTTAGYSLLLSVSEPERFAAQLRELSPAG"
BASE COUNT         2178 a         5839 c         5412 g         2377 t
ORIGIN      
        1 tcttcgtgcg cgaaggcctt tccggcgcgc cgaagctcct cctcgacccg aacaccctcg
       61 acacgaaggg cctcgtcacg gtctcctgga cctcgccgga ccccgccggg aagctcctcg
      121 ccttcggcat gtaccgcgcg ggcgacgaga actcgaccct ctacgtcctc gacgtcgact
      181 ccggccgctg gctcgccgac gagatccccg ggaaagtcgg cggcgtcgac tggctccccg
      241 actcttccgg cttcttctac cggaacctcg agaacgtgaa ggatccctac tccggccagg
      301 tcatgttcca ccgcctcggc tcccacccgc gccaggacgc ccgcctcttc cggcagtaca
      361 cgaaggaaga gaacgcgaag ctcgcgacga cctacgggcc cggtttctcc gcgagcgaag
      421 atggccgctg gggcctcctc tcctacgcca ccggcaccag ctcgaacgac ctctgggcgg
      481 tcgacctcga ccgttacttc aggaccggcg agttcgtgaa gaccgacgtc atcctcggct
      541 ccgaaggctc ctctcgcggg ccgatcctcg gcgacaccct cttcctccac acgaccgacg
      601 gcgccaagaa cggccgcgtc gtcgccgtcg acctgaacgc ccccgcccgc gccggctgga
      661 agacgctcgt ccccgagcgg aaggacgtct cgatccgcgg cgtctccctc gcaagaggca
      721 tcctcgccgt cacgctcctg aaggacgctg cgacccggat cgagctctac tcgctcgatg
      781 gccggcccct cggcgagctg ccgctcccgg gcatcggcac ggcgttcctc tcgacctcgc
      841 aggaccggac cgaggcgttc ctcacctaca cctccttcaa cgagccctcc ggcgtctacc
      901 gcgtcgacct cgcgacgaag aaggtcgagc tctgggagcg cccgaaggtc cccgtcgacc
      961 cgtcgatcgt cgaggtgaag caggtcttct acccctcgaa ggacgggacg aaggtcccga
     1021 tgttcgtcgt ccacaagaag gggctcaagc tcgacggcgg caacccgacg ctcctctacg
     1081 cctacggcgg cttcaacgtc agccagacgc cgagcttcag cgcgacgctc tttccgtggt
     1141 tcgaggcggg cggcgtctac gccctcgcgg gcctccgcgg cgggggagag tacggcgacg
     1201 cctggcacga ggccgggatg ctcgagcgga agcagaacgt ctacgacgac ttcatcgccg
     1261 cggccgagtg gctcgtcgcg cagaagtaca cgaagccctc gaagctcgcc gtctccggcg
     1321 gctcgaacgg cggcctcctg accggcgccg tcctcacgca gcgccccgag ctcttcggcg
     1381 ccgtcatcag cgccgtcccg ctcctcgaca tgctccgcta ccagtcgttc ctgatggccc
     1441 gctactggat ccccgagtac ggcaccgccg agaacccgga gcagttcagg ttcctccgcg
     1501 cctattcgcc gtaccacaac gtgaagaagg ggacgagcta ccccgccgtc ctcctgacgg
     1561 ccggcgagaa cgactcccgc gtccacccgc tccacgcccg caagatggcc gccctcctcc
     1621 aggcctcgac ggcctccgac ccggccacga agccgatcct cctctgggtc gaccgcgagg
     1681 caggccacgg ccagggcaag cccctcgccc ttcgcgtccg cgacgtcgcc gaccagcgga
     1741 tgttcctgat gtggcagctc ggcatgctcc cgaaggagtc cccgagaaag tagcccacac
     1801 gagaggtcta ccctcttcgc tctccgcgtt cccgggccgc cctcgcgggc ggcccgtcgc
     1861 attcaggcgg agccccaccc ccggatcgcg cccggaggac gagttctggt gccaggcgtg
     1921 aattcgtgta ttgtttgaaa caatacacga attcacgcct ggcacgcaac gtggggcacn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc aacgtggggc acccaacgtg
     2101 ggtggccccc atcgtgggca ccccgcgtgg cggggcagct gctgcgtgcc gcggcccggg
     2161 ccgcccgcgg atcagcgtcc gcggacctgg accgagaccg ccgccacctg gtcgatccgc
     2221 acgaccgcgt cgaagaactc ccggcccgag aggtcggaga ggtgcgcgag ctccctgccg
     2281 accttctgga gcttcccgag gacttcctcg ccggacccgg ccatgcgaag gcggaccggc
     2341 ttgccctcga gcctcttcag gacggacgcc atcgagtcct gctcttcgag aaccatcttc
     2401 ttctccgccc ccgcggggag ctgctgggcc gaagccactg cggcgagcgc cgtcgcggcc
     2461 acgacgagga cgaacggtat ccggacctgc gaacgcacga acattgcgac ctccccgggc
     2521 tcttccgtgt atcagatcct ccgactctag ccccggcccg gcccgcggcg tgtgaccttc
     2581 gtcatttcga cgccggagac tcgcgccgcg cgcgggccga ctccgggcct cgggccgccc
     2641 gggcggcgcg ggagaccgga cgcgagaatc gccgcacgat cccgatggtc ggatgctcgg
     2701 gacaccgcga cgaatccggg gtcagacgaa tccggggtca ggcgtgaaat cgtgtatttc
     2761 aaccgcggca tatcggcctg acctccgttt cgatcccgac cgtccgtccc atccagcgcc
     2821 ttcgcatcgg ggcgttcctt cggccgaatc gctgccctcg cttctcggct gccccgtggc
     2881 catcgagcga tcccccggag ccgccccgcc gcgagccctc cccgattcct cccggaagcc
     2941 ttcccgaaat tcctccgctc gctccccgcg cgctctcagg cggcgtcacg cgcacgccga
     3001 caccttcttc ccgagacccg ccgaagccga ggacgaaccc caggggccga acggcgggcc
     3061 gggaaggagc ccgccatgcg agaggccgtg cgaagtgcgt cccggtcggc cctcctcgcg
     3121 gccctcgtcg ctccgctcct cgtcgtatcg ccccgggcgt cgtccgccga cggccgcggc
     3181 ccgctcgtcg agccccgact cgggccgctc gtccaccgga ccgtcgagcg cgccgcctcc
     3241 cggctcgcgg tgcctggctg cgcgatgctc ctcgacgact tcgccgacgt ccgctccggg
     3301 gagccgctgt ctcgggccct cgccgcctcc ggacgcggac cgtccgacgt cttcgcctcg
     3361 ctccggttcg tcgacgccga ccacatggtc cagtgccgcc ggcgggcgac gtgggcgtgg
     3421 gtcccggtcg gtgccgacgt cgtcttcgtc tgccggaacc gattcacgga cctcgtccgg
     3481 aaggacgagc ggctcgccgg gaacgtcctc gtccacgagg cgctccacac gctcgggctc
     3541 ggcgagaacc cgccctcctc cgaggagatc acggcggcgg tcgtccgccg ctgcgggagg
     3601 tgaaggcgct gcggccggga gcggtgcccg tcccggcggc ggcgattccc ctcgcgtccg
     3661 cggccggccc tcgccgcagc gccgccctca gccggccgag cgcgtcggca tcgaagggct
     3721 ggcggcgaag aaccggtaga cgacgcggac gcagccgagg acgatctcgt ccccgtcccg
     3781 gagcacgacc gcttcgcgga cgggccgttc tccgactcgc gtgccgtttc ggcttccgag
     3841 gtccacgatc tccgcccggt cgcccgccac gaagacccgt gcgtgggcgc gggagaccga
     3901 gtcgtgcccg agccagacgt tcgcctcccg cacccggccg aggacgttcg gaccctcggc
     3961 gagcggaaag gtctgagccc cccagacgag gaggtgagac ctcgccgaag ggggctccgc
     4021 ctcctctcgg accggggcgt cgaacgcgaa accgaagccg tggaccgtgc gaatcagcgc
     4081 gttcggcccg tgcccgtcgc cgagcgcctt ccggatctcc ttgacgaggg agtcgatgtt
     4141 cccctcggtg acgaagactc ccggccagag cttctcctgg atcgcgagcc gcgagacggc
     4201 cgccggccgc ttctcgatga ggagcccgag cagggcaaag gccttcggcg tcaggtgcac
     4261 gggcgccccc gcccgagaaa cgcggcgggc gtcgccgtcg aaggtgacgt cggcgaacga
     4321 ggccctcatg cgtcagcctc ccggcaccgc ggcgaggatc cggtaggtga gccggatggc
     4381 cccgacccgg atctcgtcgc cgtcctcgag gacgacgcgc cctcggatcg cctccgtacc
     4441 gcgaaacgtc gttccggtcg cggcgagatc ctccagctcg gcccggtcgt ccgtcaccac
     4501 gatccgggcg tgctcgtgcg cgaccgacgg gtggcccagg cgcaccgtcg ccgagcgctc
     4561 tcgcccgagg aggttccctc cctgggaaag ctcgacgtgc tggagcccgc gcacgaggac
     4621 gtgccgtggc gccacaggca tctcgtgcgc gtcgccgtcg aaggcgtacc cgtatcgatg
     4681 gaccgtccgg atgaaggtcc gcccccgagg ccccgagccg aggacgtgcc gcacctcgct
     4741 cacgagggac gtcagcctcc ccgcggccac cccctcggcc ggccacaggg cctcgaagat
     4801 ctcctcgcgg gcgacggcgc cgggcctccg ctcgaggagg agcgtcagga gctggaaggc
     4861 cttcggagag agatgcaggg ccgtgccccc ccgatggagg agccggcggc ctccgtcgaa
     4921 tgagaagcct ccgaagcgca gcctcgggcc gaggtcctct ccggctatgg cacctcccat
     4981 cccgacctcc acaccttcga tacgaatctg gcgctgcgtc ggcggaacgg aaggacgcga
     5041 tcgacggaat cagggaaatc cgaggcgaac gaaaggaacc cggccctcct ccgagggcgg
     5101 ccggctagat cgcgctctgc gtcgagatct ccgtcgagcc cgcacggtag accaggacga
     5161 ccgagcccag gcggatctcg tcgccgtcca ccagcgtgac gcgaccccgg acgacctcgg
     5221 cgccgcggag cgttccgttg cggctcccga ggtcctcgat cgtcgccacg ggtccctcga
     5281 cggtgatccg ggcgtgctcg cgggagaccg acggatggcc gatccagacg tcggcgatcc
     5341 tctcccgacc gacgacgttc gacccctcgg caagcgcgat ctcctggctg ccccagagga
     5401 cgacgtggcg gaactcgccg gccgcctccg ccggcagctc gcggaccgcg gcctcgaagg
     5461 cgtagccgaa gccgtggacc gtgcgcacgt actccgcctc cttcgcgtgg tcaccgaggg
     5521 cgttgcggac ctcgttgacg agcgcgggaa ggttcgtctc cgagacgaag gtccgcggcc
     5581 acagcgcctc ctggatctcc gccttggaaa ccgcattcgg gcggcgggcg agaagaagct
     5641 ccaggagctg gaaacccttc ggggagacgt ggacgacgtc ggggcctcga agcagaagac
     5701 gccgtccgcc ttcgaaggtc agctctccga atcgcagcct catgtcacct cacggtcttg
     5761 tgcgaaagag tctaccggga ttccctgtgc gatgcgaacg catccgcccg ggagtgcgcg
     5821 ggatcgcgac tcgggccggg gcgccgacct cctcccccgg gcccgtccgg ctccccggag
     5881 cgctcgagcg ccttcgatcg gccctggaga gccctcgagg cgcacccgcg ggttgcccgg
     5941 acgcgaggga tcggagtaga ttgcgcgaaa agcttcttct tggcgcgggg cctcggctcg
     6001 gggccggccg tgccgacccg gaggtggcga gttgccgctc gctgcgggcc aggagctcgg
     6061 gccttacgtc gtgctggccc cggtcgcaac gggtggaatg gggctcgtct accgcgcgcg
     6121 ggaccggaag ctcgaccggg aagtcgcgtt gaaggtcctt ccggacggcc tcgcttcgca
     6181 cccgaaggct ctcgcgctct tcgaatcgga agcgaaatcg gtcgccgcgc tctcccaccc
     6241 gggaatcgtc gccattcacg atttcggacg gatcggaggg acgacgttcg ccgtcaccga
     6301 gctcctcgcg ggcgagaccc tccgggcggc ggtcacgcgg ggaccgattc ccccgccccg
     6361 ggccgtcgac atcgccgccc aggtcgccga cgccctcgcc gcggctcacg cgcgggggct
     6421 cgtccaccgc gacctgaagc cggagaacat cttcctcacg acgtgcggcc gggtgaagat
     6481 cctcgacttc ggcctcgccg tccgcgcggc tccgctctcc gcgggcgccg gggacgccga
     6541 ggtcccgacc gcccccctgg tcgaatcgtc gacgggagca ccgggaacac cggcctacat
     6601 gtctccggag caggctcgcg gcctcgccgt cgacgcctgc tccgaccagt tcgccctcgg
     6661 cgcggtcctg tacgagatgc tctcgggcct ccggccgttc cagggctcgt cccacgcgga
     6721 ggtgctgtcg tcgatcctgc gcgacgagcc cgctccgctc gccgagcgcg tcccccggct
     6781 tcccgacgcc gtccgccgca tcgtcgagcg ctgcctccgg aaacggccgg aggagcgcta
     6841 tgcctcgacc ctcgacctcg cccgcgacct cgcagactgc gcctcgcgat atcgcgaggg
     6901 ctccttcacg ccgacgccga agagctctcc cgtccttccc ccccccgcgt tctgggcggc
     6961 cgcggcggcc accgcggtgc tgcttctcgc cgccgcgttc gcgggccgca gggcggccgt
     7021 cggcgggccc gccgccacgt atcagtccct cctcgcgggc cggcagttcg tgcgcctcac
     7081 cgatcgcagc caggacgcga cgacccccgc catctctccc gacgggcacc tcgccgcctt
     7141 cgtgagacgc ctctccgcga cgaatacgga catctacgtg aaggacctga ccggcggagc
     7201 gctcgtcgac gtgaccggcg accaccccgg cgaggatcgt gagcccgcct tctcccccga
     7261 cgggtcgctc ctcgccttcc ggtccgacag ggacggcggc gggctcttcg tcgtggagac
     7321 gcaggggggg gccccaagac gcctcgcacc gtccgggcac gaccccgcct tcttccccga
     7381 cggccggctg ctcgtcttcg cgacgaccgc cacggggact ccctacgcgc ggctgggggc
     7441 gcggagcgcg ctccggaccg tcgacacggc cacgggcgtc acgaggctcc tctacgaggg
     7501 agacgcgatg cagccggccg tctcccccaa cggactgcgc gtggcgttct ggatgctccg
     7561 ggagggaggc tcgcagcgcg acctcgcgac ggtcgcggcg aggttcggcg ggccttccgc
     7621 tccacccgtg ctcgtcaccg acgaccccgc ctgcgactgg agccccttct ggtcggggga
     7681 tggccggcac ctctggttcg cgtcggatcg cggcgggacg atgaacctct ggcgggtgag
     7741 gatcgacgag gagagcggag agccgctcgg tcccccggag cccctcaccg tcccggcccg
     7801 cagcgcagga ccgttccagg ggagcgcgga cggacacgcg gtcgtctatc aggccgggac
     7861 gtcgggatac gacctcttgc gcgtgcccgt cgacgcccgg ggctttcctt cgggaccggc
     7921 ccggaacctc ctgacggacc acgagggagt cggcgagctg cggatctccc cttcgggaga
     7981 cctcctcgcg ttctccacct ggcgcgccgg cgaggacctc tgggtcgtcc gcaccgacgg
     8041 acagggactc cgcaggatca cctcgagctc ctttcacgac cgcgcgccga gcttcaccgc
     8101 cgacggcagg tcgctgatct tccactccga ccgctcgggg aagttcgaga tctggaaggt
     8161 ctccctggac gggggcggcc tcgcgccgct cacgcgcctc gacggcctcc atccgttccg
     8221 cgcgctcgcc tcccccgacg ggcggctcct cgccgtgaac aacagccgga cgggcgccct
     8281 cctcctccct tcggacgccc cgcccggagc ctcctggccc gcgccgttgc ctcctccgga
     8341 acccggcctc cgcttcgagc tgacgtcgtt ctctcccgac ggccgcttcg ccgcgggcca
     8401 cgcgatctcg ggcgacgagc gctacgccgg ggtcttcctg cacgacctcg gatcggggcg
     8461 ctacgagcgg ctgaccgccg ccggctccag cccccagttc gtcctcggcg ggacgcggct
     8521 ctacttcctc cggcagacct cgctcttcgc gccttcgacc ctggcgttcc tcgacctcgg
     8581 aacgcgtcgg atccacgacc tgcagcccga ggggaccggc agctccatcg tgtcgttcac
     8641 cgccgccccc gacggctcgg cgctgtacct cgtccgggag agaagccgaa acgacatctg
     8701 gctcatgcgg atgccctgac cgttccgtca ttctcccgag agcccggctt cccccttccc
     8761 gcggggcccg ggttcttccc ggaggcgtcg ggcggccccg gcgcggagct cccgcgcggc
     8821 cgcccgttcg ccgatcgcct cgaggctccg gattgcggcg gcgaccccgc ccggtccggg
     8881 cacgttcgcc gcgatctccc gggccgcagc gagcgcgccc tcccgccggc ccgtcgccgc
     8941 tcgcagggcg acgagcgcgg cgcgcgcctc gacccggacc gggaagagcc cgatctctgt
     9001 gaggagctct tcctccgcct ccccggcgcg tccgagccgg gcgaggacgt cacccctcac
     9061 gaaatggagg cccgcgagct ttcgcgcctc ctctcccgag acgtcccgcg cccgattcgc
     9121 cagcttcagc gcgcccggca ggtccccgcg aagcgtcgcg acgcgggcca gaacgagaag
     9181 cgcccgcgcg ggggaacgtc cccgcgcgag cacccgcttc gccgcctcct ccgctccgcc
     9241 gaggttcccc gccgccaggt gagcgcgcgc aaggacgtcg tcggccgccg ggtcgttgca
     9301 ggcgcgggcc gcctcggcgt gccggagggc cacctcgggt ctccccgccg agagcgccac
     9361 cgaggcgacg gacacgagga cctccgaggc cccgggtgcg gagaggggga gcatcctctc
     9421 ggcggcctcg agggcctcgc cgttccggcc ctcaccgagg agagcctgcg cgtagacctc
     9481 ccaggcgtcc cgcacgcgcg ggttgcgatc gaggaaggga gccaggaccc ggaccgcatc
     9541 cgcggggcgc ccggcccgga gggccgccat gccgtcgcgg agcggcgcga gcgtggcgat
     9601 ccggtccttc gggtccggca gggcgtcgtc gcgaacgccg gacctggccg tcacgtagcc
     9661 gagggacgcg agctgccggg ccttctcggc gtcgaccggc gacggctccc ggaagcccga
     9721 acggcgcgtc tccagctcgg cccgcatcga ccggatcggc ccgtccttcc cggcggcccg
     9781 gttcgtccgc tcggacgggt cctcgagaag gtcgtacagc tccggccggg gggcatcgac
     9841 gaagtgccag cgctcgtcgg cgagggacgc gagcgggctc cagccgaagc gcgtccgcgg
     9901 gtagaacgtc tccgagagga tccgtcgccg gggtccctcg cgcccgctcg ccagctccgc
     9961 gagcgagacg agcccgggcc ggtccgggca ccctttcagc cccagcgccg agcagacggt
    10021 gcggaagacg tcggtcagtc cgaccggcgt cccgaccgtc gtcccgccga agaggttgcc
    10081 cggcagcttc acgacgacgg ggacccggat cgactcgcgg tacaggaaga tcccgtgctc
    10141 cttctctccg tggtccccga gcccttcccc gtggtcggaa agaaagacca cgaggctgcc
    10201 gtcgtacagg ccggcggcct tcagcccggc gacgaacgag ccgacggcgg cgtccgcagc
    10261 ggcgatctcg ccgtcgtagg gatcggtcca ccggctgcgg aagggctcgg gcggagcgta
    10321 cgggctgtgg ggctcgaaga ggtggaggaa ggccaggaac ggcgcgtgcc cctggccggc
    10381 gatccagcgc gagagcgcac gttccgtggc cgcgcccggc cgctcgagct gcgtcatcgg
    10441 ctcgcccggg gcggcgtccc cgaggtcgtc gtcccagaag tcgaaaccgc gcgcgatccc
    10501 gctccgcccg tcgaggacga cgctcgagac cgcgccgccg gtggcgtagc cgttcgcctt
    10561 cgcgatctcg gcgatcgtcg ggacggaggg ggcgagcgcg tagcccgagt tgtcgagcac
    10621 gccgtgctcc cgcgcgtccg tcccggtcat cagggcggca tgcgatggga gcgtcagcgg
    10681 ggcctgggcc caggccgtct cgaagaggac cccgtcggcg cgcagcgacg cgagagccgg
    10741 cgtcgcgacc ccgccgtacc cgtagagagg cagccggtcc gcgcgaaggg tatcgaccga
    10801 gatgaggacg accgacgcgc ccgggaaccg gctcggcgcc tccggccgcc ctcgcgcgca
    10861 cccgatcgcg aggaggaagg ccagtgcgat cgaggagggt cgcgtcgtga ccgcctccgt
    10921 acgccgagga tatccgcggc ccccgtccgg cggatcggga atgggagccc cggctcgcgc
    10981 cggggctccc ggggattcgc ggaggtactc gctagaagcg gacgccgaac ccgatccgga
    11041 agaggcgcgg cgcctggtag ctcgccgagc tcgtcggctt gccgaagctc gggccgtagt
    11101 tccagttcgc gcccgtcccg cgggctccct gcgtcggctg ggccgtgaac gggttgaagg
    11161 cgacgtagtc gttacggcgg ttgacgcggg tctcgatcgt cgtgttcacg gcgatcacgg
    11221 cctcgttgcc gaagacgttc gtcacctgcg gctggacgaa gatctcgacc ggcccgatgt
    11281 tgtaggaata gttgagggcg aggtccgtgc ggtgcgtgtc cggcaggcgg aacgcgtcac
    11341 gcttcgtgaa gtagtagttg accgaggagg ggggcgtctc gtacccgacg tcggccacgt
    11401 agggccagct cgcgaccgac ccgacggcgc cgtagggcgt gccggagttc caggtgtgga
    11461 ccgcggagag gttgagcgcg ccgaggacct tcgggagggg gatgtcgtag gtcgcgtagg
    11521 cgacgaggcg gtgcgtctgg tcctgcggca ggctcccggt cgggatgttc caggcggggt
    11581 cgaagtactc ggggtaggag gtccagccga aggaccgcgg gccggagcct cggtcctccg
    11641 tgacggcgtt cccgtaggtg tgcgagtagg tgtagttccc gccgatgttc aggctctcga
    11701 agagccggta ggccgcctgg acctggagag ccgcgtaggt cctctcgatc gcgttcgtgt
    11761 tctgcaggag cgcgaggtcg tacgtgatcc cgaccgagtc cgtcaccttg cccgtcgtgg
    11821 tgtcggtgaa ggtgtcgatg aagtcgccga acctccggta gatcccgtcg agccggaagt
    11881 tgccgcgggt gccgatggcg cccgccaggc tcagggcgta ctcgtccgtg taggtcgact
    11941 tcaggctctc gcggatctgc gtcgagacgc ccgcgaccgt cgcctggatg ggcgagatgc
    12001 cggggcgcgt tgggaactgg ttgatgccgg tgatgccgaa ccagctgaac atccgctcga
    12061 gggtcgccgc cgtcgtgacg ggactcgacg ggtcgccctc gttgatcggc tcaccctcgt
    12121 agtaccagac ccagatctcg gggctgccgg cgctcgacgc gccgccgatg aagttctcct
    12181 gctggccgcc cacgtacctg gcgtagctcg cgccgacgcg cagccggccg tcccccttga
    12241 cgtcccacgt ggcggcgagg cgcgggctga aggcgctgtc gtccgaggtc gtcacgcccc
    12301 ggctgtcctt cccgtcgttc ttgtcccagc ggagcccgat gttgaaggag aacctgtcgt
    12361 tcatccgcca ggtgtcgttc acgtagatgg agtgcgtccg gacgttgctc cccttgctca
    12421 gctcgaagat cggccagtag acgaggacgg agtcgttccc gaggaccggg tagacgttct
    12481 gcccctggac gatcgtggcg gacgagtaga cggtgtagtt gctcccggac tggtagttgt
    12541 tcgagtagac cttgttgttg aagttgtcgt atccgaagac gacgttgtgc gaaccgaggc
    12601 tcttcgtcga gaggaaccag gtccccttgg cgaggacgtt ctcgttgtcg cgggtctcgg
    12661 gctcgcacac gccgcagaac ggggaggcgt tgtagaggcc gccgagcgtc ccgtcgaaca
    12721 tgttcgtccc ggcgatcagg tcccgcgagt agccgcccgc gttctcgaac gcgaacttgc
    12781 gctgggagta ctgcgcctcg acgaagacct ggtccgtcag gaccccgttg tagttgaggg
    12841 cgaggagcga ctgggggtag ctcgggttgt agagggtctt gagctccatc ggggagtacc
    12901 cggggttgac gttcgtcgcg tcgttcgcga tgtcgatata ggaggccgtg aacgtgtggt
    12961 tctggaacgg cgtgaccgtc agcttccctt cgagccgcag ctcctccgtc ttcgacgggt
    13021 atccgacgtt cgtgaggtac gtctgcccgg tcgtttccgt gtcgcggctc cggcccgcgg
    13081 cgaagaacca gatccggtcc ttccagatcg gccccccgag cgtcgcctcg taggtcggga
    13141 tcgtcttgtc ggtgtagtcg acggtctgga gaggcctcgt cgcctgccag ctgtcgttgt
    13201 tcagcgtggc gcgggcggag ccgctgaagg tgttcccgcc cgacttcgtg atcgcgttga
    13261 cgacgccgcc cgtgaatcgc ccgtactcgg cggagatcgc ggccgtggag gtcgtcgtct
    13321 cggcgatcgc gtcctcgatg aacaggtccc tcggcgagcc gcggatgttg tcctggacga
    13381 cgacgccgtt gatcgtgacg aggttgtcgt acgactcggc gcccgagatc gtcgtcgcgc
    13441 cgcccggccc gttcccgttg acgccgggcg acagcgcgac cgcggaaagg agcgtccggg
    13501 tcaccgggag cttcttcgtc aggtcggagg agatcgtcgt ggcggcctgg gtcgtctggg
    13561 agatcgactc cgacgtcgag acgaccgtca cctccgtggc cacggccgag agctgcatgt
    13621 tgacgttgac gatggcggac tgcgaggcgt tgaccttcag attccgcgtc accgtctgga
    13681 acgaggtcat cgtgaacgtg agggtgtagt cgcccggggc caggttcggc agggcgtagt
    13741 cgccgttcac cgaggtgatg gccgtgcgcg ccccctggag agacggcgac ttcgccgtca
    13801 cgaccacacc cggaaggccc tgcccctcgt tgatggcctt gccgctgatg gtgccggtcg
    13861 gcaggccctg cgcgaggagc gaggcggtga ggaagatggg caggacgagg aggagaagac
    13921 ggaaccggcg gagtctcatg gcgggctgca ctcctttctc cggggttgcg cgtcccgctc
    13981 ccgccggagg ggcgggaacg acacggtgat tggaagcgtg cttcggcacg ccttcgggga
    14041 aacgtggcga atggggctcg ggtcgttctc tcgggcgacg cagcgcggca cctccttcgg
    14101 gtccccgtcc gccggcaggg aaccccgcgg ttcacaggtc ctcgacacgg gcgcggagcg
    14161 cccgcccccc atttcacgta ccgatccatg gatcggtatc gacgtaccgg aacctgcatc
    14221 gctcggcgcc ggtccgagtc ggggcacgcg accggctccg tccgctcggg caggctcgac
    14281 gctgcgtcgt tcagcggttt cgacgccccc ggcgcgcgtc gcgctgcggt cgggcccgct
    14341 cgcgattccg gtcatgcccg tttcgactga gaagcgagtg ccactccggg gcgtttaacg
    14401 ggattccaag cgctttccga tgacaccgcc ccgtcccgat cgcacggact gaaccgggcg
    14461 gcggcgccgc tctccgggcc gaggcccggt cccttcccgg ttccggcctt tcccagccga
    14521 ttccaaggcg caacccagcg aaatccccgc ccgctccccg ctttctctcg ggtctcgccg
    14581 gggccgcacc ggcacctttc cctcgggttt cgggcgctgc ggccgaaggg gaacgaggcc
    14641 gcggggcgaa ccgggaagga ggccctccgt gagcgaggcc gtgcgaggtg tgtcccggtc
    14701 ttctctcggc gcggcgctcc tcgtcgcgct cctcccgtgg acgacgaccg gcgcggacga
    14761 gccccttcgc cccgaggtcg acccacgcct cggcgggtac gtggcccagg tcgtctcgcg
    14821 cgcctcgcgg cggtttgccg agcccggttg tgtcagcctt ctcgtccgct tcgaggacgt
    14881 gcggacgggc cgcccgctct cagagaccct ccggacttcc ggcgagactc ccgcgagcca
    14941 cttcgcgaag ctccgcttct gctccggaga cgagaggctc cagtgccgcg gacgcgagac
    15001 ctgggcgttc accggaatcg gctcggactc cgtctttgtc tgccggagcc gtttcacggc
    15061 tctcgcgaag aagagcgaga aggcggccgc gaacatcctg gttcacgagg cgctccacac
    15121 gctcgggctg ggcgagaacc cgccgacctc gcgggagatc acggccgccg tccacgacca
    15181 ctgcggccgg tgagccaggc tctcgtcgcc gatgcgcgct ccgcgtcgct cgcggctcgg
    15241 cgagaatggg ccatggagac cgtctacacg ctcgtcccgc cgccggggaa gacctccacg
    15301 gccctctgga tcccggtcgt cctcgtcctc ctgatctcgc tcgtcgggat cttcttcgtc
    15361 tacaagtccc tgtcgggggc gcgaggctcc acgttcacgc tctctcccgc cggactcgtg
    15421 atccgcggcg acctctgggg ccgcacgatc ccgacggcgc agctgcgcgg cgccctcgcg
    15481 cgcgtcgtcg acctgcgcgc cgagacggcg ctcgcgccgc gacggcgcac cgcgggcacc
    15541 ggcctgcccg gctaccggag cggctggtgg aagctcgaga acggcgagaa ggcgctcctc
    15601 tacgtcacga cgaccgaccg cgtcgtccac gtcccgacga cggcgggcta ctcgctcctc
    15661 ctctcggtct cggagcccga gcgcttcgcg gcgcagctcc gcgagctttc gcccgctggg
    15721 tgagccgcgg gggcggaggg ctcacttcca ggcgatcgac gcgaacacga gagacggcga
    15781 gaggagcgtc ccgacccgcc ctccgggctt cagcaggtcc gccggaacga ccgagccgtc
    15841 ccccgaccgc accttctgga ccgccgggag gaggaggccg atcaggatcg cgatgatccc
    15901 ctgccg
//
LOCUS       DAOTEZ010000043        15790 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2534_length_15790_cov_6.790149, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000043 DAOTEZ010000000
VERSION     DAOTEZ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15790
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2534_length_15790_cov_6.790149"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="P5164_03640"
     CDS             complement(<1..321)
                     /locus_tag="P5164_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43021.1"
                     /translation="MRARTGLRLLALPLALLLVPAGSLADDAPDAAARLKALEERIES
                     LEFDLDQVRKSSDDALFWLRLGDVAEIDKVNVAGPPNPRAKERYGIANERHPIRFAQY
                     VFAPK"
     gene            479..1228
                     /locus_tag="P5164_03645"
     CDS             479..1228
                     /locus_tag="P5164_03645"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRY43022.1"
                     /translation="MAGRTIAIGDVHGDLDGLERLLARLPVLLPEDTLLFVGDYVDRG
                     PHPAAVVRRVRALPSETAARVVFLRGNHEDAWLKACREGAPEFVLPRGNGCYSTYRSF
                     TGGPVPDPGENPTAEELRAMATGTFFPADVVAWMEGLPLFHEDEHAIYVHAGLPHDGR
                     RWLHPSEVKDPRPLLWQRSRSFYEGYTGKRVVFGHTPVRKLPQDQSVHTPGDPLDVYV
                     TASLVGVDTGAGMGGFLSAIELPEMRVLDAR"
     gene            1289..2449
                     /locus_tag="P5164_03650"
     CDS             1289..2449
                     /locus_tag="P5164_03650"
                     /inference="COORDINATES: protein motif:HMM:NF014511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRY43023.1"
                     /translation="MPLIPLSPIDHVFTGRGAYPIEFVFAYEGAIDAGRLEASLRKAI
                     DAFPPAGSRFVRLDGAALAFEPSEGGCVFSVASSPTEWEATERKDVFLDPVSGKEGEP
                     LGRALLTRTPNGCVLGFSLSHAVADGYSYFHFLTSWARLFRGEPILPPTHDRAALAAG
                     LDGTRDGDAADAAGLFRAGPRPEVDRAHLTIHRHVLTRAELVARHAEAQAGNSLRLSH
                     NDTAAAWLWKTRLAEWSGDDPSLETYVSCPVDFRRLLPSTGPAYFGNAVALAKASIPK
                     GELAAAPLGELATRVRRAVDAVDADAARRSLGALETLRRSEGLAAMEECHVVHPMQGL
                     LLTNLSRLPVAEVAFDAGPPVAFEILTPGVRGAVALPAPDGGLDLRVFLPSA"
     gene            2446..3315
                     /locus_tag="P5164_03655"
     CDS             2446..3315
                     /locus_tag="P5164_03655"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRY43024.1"
                     /translation="MSHNRAVIHRRFTLASRLGEPIHGDLRLPDGSGPHPVVVCCHGF
                     KGFKDWGFHPWLGGRLAEAGLAAIHFNFSRNGVRAADGDIEDLDAFRRNTLSIERDDL
                     DTVLDAVLSGAIDPSLDATRLGLFGHSRGGGIALLGASERPEAKALVTWAAVSHFDRI
                     ADEAALTEWRRTGVFEVVNSRTGQRLPMGVAFLDDVLANLARLDLSAAARRLAVPWLL
                     VHGTADPTVAFDEANELHAAAAGPARLLPLDGAGHTFGAVHPFSGPTPHLAAAAEATL
                     AHLRSALAGASPA"
     gene            3324..4676
                     /locus_tag="P5164_03660"
     CDS             3324..4676
                     /locus_tag="P5164_03660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY43025.1"
                     /translation="MIDGPASRPRRSAAVAAVYLLAFVWILGKAALFRERVPPGVPPD
                     EPVHVSYVAWLEATGALLPRYEEMHLLEADGRFGAQPSYLAHPPAYYHLLRGLARATG
                     GIPAGTLDAATRRLRALSAPLFAAAAALFLWLGFRRAAPLPAHALFAATIATVPPLAF
                     VGSAVNNDVLAFLAGGLALLGLARLLEGRAGGATGALVGAGLALALLAKATAGLLVLL
                     AVAAALALARRTVRWERDPRFLLALLSWLLLPALHYVPILLRYGTPVPSLDVVDPEAF
                     ARSAFVDAPGAPRLAVVPWAVKLLKVFGSTWLSLIAHVWLPVGPLPTLAGPALAALLA
                     ARGLLRRRRSDGAAEAPLDDLVRAGAVALAGTLLVNLAWARDGYLATERLGGMHARYY
                     LPLFPVLAFAAARGLERIPARGLARLTGRGLVAAAAVLLLVLFDAGVTARYLALFPAP
                     "
     gene            complement(4693..5142)
                     /locus_tag="P5164_03665"
     CDS             complement(4693..5142)
                     /locus_tag="P5164_03665"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRY43026.1"
                     /translation="MNPSESPTPSQERLDALRAAEHPRCFLCSPDNPAGLKLRFRVQP
                     DGSVAAFFPCREVFQSYPETLHGGIVAALLDAAMANALFAIGVVAVTAEMDVRYRAPV
                     ALERGVVVRGRVEKTTAHPLYYVRGEVEQDRKVVARASARFLARDGV"
     gene            complement(5139..5879)
                     /locus_tag="P5164_03670"
     CDS             complement(5139..5879)
                     /locus_tag="P5164_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43027.1"
                     /translation="MATVPVDRRLLCALAGVAGLSLLAVAYLLGRANGGAERAAPRLE
                     GAAPAFAATPAAAGAAALTAPVPPPSHAEPAYVELPSSPATQAAPEVGPELRAPFLPT
                     PLPAPDLAPPDDPERAAIAAYLDAVDAIQASGLAGSPESVANEMAVALARGDASGLDG
                     LVREAEEARRRLLGISPPAACAAHHRETLGSFDDALAMLRALKSATGSPDPASALAEV
                     AARATALKARAESLKTEEEALRRRGPTR"
     gene            complement(5892..6866)
                     /locus_tag="P5164_03675"
     CDS             complement(5892..6866)
                     /locus_tag="P5164_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HRY43028.1"
                     /translation="MTKRPRVPTLLLALALIATGTLAAAPPGPSTFSIAAADPATGEV
                     GVAVASRFFAVGTVVPWAKGNVGAVATQASANTSFGPDGLDLMARGLSAEEALKVLLR
                     GDEGRERRQAGLVTASGDAATFTGSGANAWAGGRKGPGYAVQGNILVGEAVVEAMERS
                     FLASAGKPLAERLLAALAAGDAAGGDSRGRQSAALLVCRAKGGYNGFTDRAVDVRVDD
                     HADPFRELARLVGMAVVNDLWNRGWTGFMEKRYAEALKWQEEATARAEAQPAMLPEVV
                     YDLGVIRLAAGDRAGALEAARRALRLNPKLAAQAAVDPDLAAIRAELR"
     gene            7095..8462
                     /locus_tag="P5164_03680"
     CDS             7095..8462
                     /locus_tag="P5164_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43029.1"
                     /translation="MTKARVVGGALSVALFLLALVFLVRHRTVAPPPASPAEEAGSTA
                     PAAAEKMEERDAQGFLHGRVTTLTGATWEGRLRFGGDQEAFWSDAFNGRKVENAWVRH
                     VPPDRLPRERRGFSVFGLELGRRERPVSFVRLFMARLGDLSRIEAHGGRVRVTLKSGT
                     VVDLDRLEASDFDDGVRVWDPRRGVADLDSARIRVIELLPGPRTGRVPTRLQGTVRTA
                     QGSFRGDLQWDRESCVGTDELPGRTTEGVRRVRFDAIRSLERREGAVLLVTNHDGRQL
                     SLARAPERGWGGRGTYVDDPRYGRVLVPWEALERVDFEPGGSGPSYEDFPPGAPLSGT
                     VSTRDGRRLSGRLVYDLDESEVTDTLDAPSQGVDYMLPFGLVASIELPGADERPAPRA
                     RVTLHGGEELRLELAGDLGERNAGLLVFAADRPAPEFVAWADVARVDLDRPKSFFPPP
                     GGLDE"
     gene            complement(8463..9131)
                     /locus_tag="P5164_03685"
     CDS             complement(8463..9131)
                     /locus_tag="P5164_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015109787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HRY43030.1"
                     /translation="MSERRSGYGQYCPISRALDLLGERWTLLVVRDLLVGTTRFNELA
                     RGLPGLSRSLLSQRLRQLERAGLVERLEGEYLLTAAGRELRPIVFALGEWGARWTFGE
                     PRREELDAALLVWWMHTRLDTSELPGRRQVFHVRFSDDERRFWIVVEPGGPSVCHVDP
                     GFETDVTISSDVASLYQVWLGRLPLEEAVRSGRVSFAGPVALTRRMPTVLRLSPVAEA
                     VRSA"
     gene            9230..10369
                     /locus_tag="P5164_03690"
     CDS             9230..10369
                     /locus_tag="P5164_03690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019046966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRY43031.1"
                     /translation="MTTMQDTTTTATDWLALARRIGETLEPGVAERDRLGEIDLAAFE
                     AFRAEGVTAALVPAEAGGGGASHAETGRFLRELGRHDGASAVALSMHMHLVAAQVWRH
                     RHGLDASALFRKVVEGRAVLVSTGAADWIGSSGSARKADGGWRVNARKSPASGCEAGT
                     VLVTSARWELAPGGPRVLHFAVPMTAEGVRIEKTWDTLGLRATGSHTVVLEEVFVPDA
                     AVSLERRADVWHPVWNVILGAAMPLILAAYAGIADAAVARALEAAKGRTDPHVLQLLG
                     EMLNTHTTAVDTVDAMFRDADDLRFEATDSFAGRALSRKTVAADALIATVRLAIEATG
                     GGGYARSSDLERLYRDVHGVLFHPLPRARQTTFSGRAALGLAPVG"
     gene            complement(10405..11169)
                     /locus_tag="P5164_03695"
     CDS             complement(10405..11169)
                     /locus_tag="P5164_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRY43032.1"
                     /translation="MDSSDRDREAESPRLYRDLAEWYPLLTPVEDYVEEAAFYRRLFE
                     AHAARPPRTLLDLGCGAGHNAFHMKPGLEATLVDLSPAMLDLSRRLNPECEHVAGDMR
                     SVRLGRLFDSVLLHDAVSHMTTRDDLAAAVATAFAHTAPGGVALFQPDCVTETFEEGS
                     DSGGSDGGGRGLRYLEWRFRPDPKAERYVVEMAYLLRDAGGDVRVAHDRLEMGLFPRT
                     FWLETLAAAGFEALVVPLEHSAAGEYEVYVGRRLVR"
     gene            complement(11310..11384)
                     /locus_tag="P5164_03700"
     tRNA            complement(11310..11384)
                     /locus_tag="P5164_03700"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11350..11352),aa:Asn,seq:gtt)
     gene            11519..12490
                     /gene="gluQRS"
                     /locus_tag="P5164_03705"
     CDS             11519..12490
                     /gene="gluQRS"
                     /locus_tag="P5164_03705"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HRY43033.1"
                     /translation="MDRGGTGRVAAEGGRLAPPRGRFAPSPTGPLHLGSLVAAVGSWL
                     FARSAGGEWLVRMEDLDGPRVVPGAAGEILRALERYGLTWDGEVAVQSRRTALYEEAL
                     ARLRTAGHAYDCGCTRAEVARAAGAPDASDPVDAAGAVYAGTCRAGLPPGRAPRAVRF
                     RAPEGTVVFEDRVRGRIQENVARAVGDFVVRRADGPFAYQLAVVVDDAAQGVTEVVRG
                     GDLLSSTARQIALQRALGLPTPSYAHLPLVLGPDGRKLGKRDGALPLETLDEARVRET
                     LGLALRLLGQEPPDGPPAEMLAEAKARFETKRIPAGPVHVGAYGASA"
     gene            12487..14349
                     /locus_tag="P5164_03710"
     CDS             12487..14349
                     /locus_tag="P5164_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43034.1"
                     /translation="MTALSASVLGAGLLRLVLAAIALSALALVGYGLFRVAGRMMGRG
                     RAPACPEAGPRLPGLRRAEVALPLALSAGAFAVGWLSFVAALVSTWIVPFVWAGLVLL
                     SLPATRDLARDAATVGRRLRALLLASPVASAMLGLALLALLPPLLLPLWDSDGLRYQV
                     ALPKLFLLEGRVVACPWDVHAALPQLVGGLLLALLPVGGGEAAKLLHAAFFVAALATL
                     ALLLHRDRRSRRAAVYGPLLLAIAPVAAVPATAAFVDHAALFHLAVAGLLVARGAPGG
                     AALPLGAAVATKTTAGPGAAALLLLASATARRGERFRVLAAGAGAILLAFAPFGLRNV
                     LETGDPVFPVGHVLLGVPVPGTDPALVRRVLEYRPDAVAPLGIGWLPGEPGLAADDVA
                     GPALFLGLLALPVTLRDRRGRVLLALAGAYLLVGFVAQPLARLLMPLFLALAGAAALA
                     ADRWLRRAATPVVAALAAGTFAFATLPALGPSAALAHLAGRLSRDTFLAAEVPGYRAA
                     LAVNALPGGRVMALDFPGPYYLARPWIAEGVVNEPPLRLWLAGGADGDGLLERCRALG
                     VTHLLVTPGWGGGTSASLFPLARSREEAKAVVAFRSRLRLVATVDGVDVFELPR"
     gene            complement(14297..15214)
                     /locus_tag="P5164_03715"
     CDS             complement(14297..15214)
                     /locus_tag="P5164_03715"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HRY43035.1"
                     /translation="MPARGAGRRSGAPAASERRRAPASPLRTVLLVGGAGRMGRLLAR
                     AFRAAGHAVAIHDPGRRLAGFPSVPLETAAGADAVVVSVALDATPAVLAEVLALSPPG
                     LVLDVASVKGPLLPLYAKAARRGLRVASVHPMFGPGVRTLAGQNLIVCDAGVPAAARA
                     AARLFSGQGLKLATLPIGAHDAWVARTLGLAHLLSLLAGSTLAREGVRVKSLDGLAST
                     SFRNLCLLAAPILHQAPDLTLPIQARNPATPGLFDLLDEELASWRALVERNDLAAFDR
                     KLAEARSVLAAPVSVAARRRPPRRPSRRA"
     gene            complement(15219..>15790)
                     /locus_tag="P5164_03720"
     CDS             complement(15219..>15790)
                     /locus_tag="P5164_03720"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012788.2"
                     /note="catalyzes the formation of tyrosyl-tRNA(Tyr) from
                     tyrosine and tRNA(Tyr); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRY43036.1"
                     /translation="PLLVGLDGVEKMSKSLGNHVGVSDPPDEIFGKVMSVSDDMMWTW
                     WTLLTDLFPHDIDALKADVASGAKHPKRVKMDLARQLVTDFHGAEAAATAEAEFERRF
                     AKAEGPVKAETVALPDPAPADLASLLVAVGLAASKNVARQKVKEGAVATSEDGVAFTK
                     ADNPGEPFALEPGAARYVRLGKRFLRVAR"
BASE COUNT         1787 a         6005 c         5852 g         2146 t
ORIGIN      
        1 cttcggcgcg aagacgtact gcgcgaagcg gatcgggtgc cgctcgttcg cgatgccgta
       61 ccgctccttc gcgcgcgggt tcggggggcc ggcgacgttc accttgtcga tctcggcgac
      121 gtctccgagc cggagccaga agagcgcgtc gtcgctcgac ttgcggacct ggtcgaggtc
      181 gaactcgagg ctctcgatcc gctcctcgag cgccttcagg cgcgccgcgg cgtcgggggc
      241 gtcgtcggcg agcgatccgg ccgggacgag gaggagagcg aggggaaggg cgaggagacg
      301 gagcccggtc cgagcgcgca tggaaccctc ccggaggacc gcggctcgct ggccggtgcg
      361 gtccggcccg tcagggtagc agccgagggg ctcccggcgc tggcctttcc cggccagccg
      421 catcgtccgc cgtccgctcg agcgcctcct ccggtatcgt gcgcgcaacg agaccacgat
      481 ggccgggcgg acgatcgcga tcggagacgt gcatggagac ctcgacgggc tcgagcgtct
      541 cctcgcgcgc ctgcccgtcc tcctcccgga ggacacgctc ctcttcgtcg gcgactacgt
      601 cgaccgcggg ccgcacccgg cggccgtcgt ccggcgcgtc cgggccctgc cgtccgagac
      661 ggcggctcgc gtcgtcttcc tccgcgggaa ccacgaggac gcctggctca aggcgtgccg
      721 cgagggcgcc ccggagttcg tcctgccgcg cgggaacggc tgctattcga cctaccggtc
      781 gttcacgggc gggccggtgc cggaccccgg cgagaacccg accgccgagg agctgcgcgc
      841 gatggccacc gggacgttct tcccggccga cgtcgtcgcg tggatggagg ggcttcccct
      901 cttccacgag gacgagcacg cgatctacgt ccacgccggg cttccgcatg acggccgccg
      961 ctggctccat ccgtcggagg tcaaggaccc gcgcccgctc ctctggcagc gcagccggtc
     1021 cttctacgaa ggctacacgg ggaagagggt cgtcttcggc cacacgccgg tgcggaagct
     1081 cccccaggac cagtccgtcc acacccccgg ggacccgctc gacgtctacg tcacggcgtc
     1141 gctcgtgggc gtcgacacgg gggcgggaat ggggggcttc ctctccgcga tcgagctccc
     1201 cgagatgcgc gtcctcgacg cccgctgaag gggcccgccc ggccagggac gggcgcggtt
     1261 ccttcgtgcg tgggcgatca tccgggccat gccgctcatt ccgctctcgc cgatcgacca
     1321 cgtcttcacc ggccgcggcg cctacccgat cgagttcgtc ttcgcgtacg agggggcgat
     1381 cgacgccggg cgcctcgagg cgagcctccg gaaggcgatc gacgcgtttc cgccggccgg
     1441 gagccgcttc gtccgcctcg acggggccgc cctcgccttc gagccgtccg agggcggctg
     1501 cgtcttctcg gtcgcgtcct cgccgacgga gtgggaggcg acggagagga aggacgtctt
     1561 cctcgacccg gtgagcggga aggaagggga gccgctcggg cgagccctcc tgacgcgcac
     1621 cccgaacggc tgtgtcctcg gcttcagcct ctcgcacgcc gtcgccgacg gctactccta
     1681 cttccacttc ctgacgagct gggcgcggct cttccgcggc gagccgatcc tcccgccgac
     1741 gcacgaccgc gcggcgctcg ccgccggcct cgacgggacg agggacgggg acgcggccga
     1801 cgccgccggc ctcttccgcg cggggccgcg ccccgaggtc gaccgggcgc acctgacgat
     1861 ccaccggcac gtcctgacgc gggccgagct cgtcgcacgg cacgcggagg cgcaggcggg
     1921 gaactccctg cgcctctcgc acaacgacac cgccgccgcc tggctctgga agacgcgcct
     1981 cgcggagtgg tccggcgacg acccgtcgct cgagacgtac gtcagctgcc cggtcgactt
     2041 ccgacggctc cttccttcga cgggcccggc ctatttcggc aacgcggtcg ccctcgcgaa
     2101 ggcctcgatc ccgaaggggg agctcgccgc ggccccgctc ggagagctcg cgacgcgcgt
     2161 ccgccgggcc gtcgacgcgg tcgacgccga tgccgcgcgc cgctcgctcg gcgcgctcga
     2221 gacgctccgc cgctccgagg ggctcgcggc gatggaggag tgccacgtcg tccacccgat
     2281 gcaggggctc ctcctgacga acctctcgcg cctccccgtc gccgaggtcg ctttcgacgc
     2341 cggcccgccg gtcgcgttcg agatcctgac gcccggcgtc cgcggcgccg tcgcgctccc
     2401 tgctccggac ggcggcctcg acctgcgcgt cttcctcccg tccgcgtgag ccacaatcgc
     2461 gcggtgatcc accgccgctt caccctcgcg agccggctcg gcgagccgat ccacggcgac
     2521 ctccgcctcc ccgacggctc cggcccgcac cccgtcgtcg tctgctgcca cggcttcaag
     2581 ggcttcaagg actggggatt ccacccctgg ctcggcgggc gcctcgcgga agcggggctc
     2641 gcggccatcc acttcaactt ctcgcgcaac ggcgtccggg cggcggacgg cgacatcgag
     2701 gacctcgacg ccttccggcg gaacaccctc tcgatcgagc gcgacgacct cgacacggtc
     2761 ctggatgcgg tcctgtcggg ggcgatcgat ccctcgctcg acgcgacccg gctcggcctc
     2821 ttcgggcaca gccgcggcgg cggcatcgcc ctcctcggcg cgtcggagcg ccccgaggcg
     2881 aaggcgctcg tcacctgggc cgcggtctcg cacttcgacc ggatcgccga cgaggcggcg
     2941 ctcaccgagt ggcggcggac cggggtcttc gaggtcgtca actcgcggac ggggcagagg
     3001 ctcccgatgg gggtcgcgtt cctggacgac gtcctcgcga acctcgcccg cctcgacctc
     3061 tccgcggcgg cccggcgcct cgccgttccg tggctcctcg tccacgggac ggccgacccg
     3121 acggtcgcct tcgacgaggc gaatgagctt cacgcggcgg cggcgggacc cgcccggctc
     3181 ctcccgctcg acggggcggg gcacaccttc ggcgcggtcc acccgttctc cgggccgacg
     3241 ccgcacctcg cggcggccgc cgaggccacg ctcgcccacc tccggtcggc gctcgcaggg
     3301 gcctcgccgg cctgaccgac gcgatgatcg acgggcccgc gagccggccc cggaggagcg
     3361 ccgccgtcgc ggcggtctac ctcctcgcct tcgtctggat cctcgggaag gcggctctct
     3421 tccgcgagcg cgtcccgccc ggcgtcccgc ccgacgagcc ggtccacgtc tcctacgtcg
     3481 cgtggctcga ggcgacgggg gcgcttctcc cgcgctacga ggagatgcac ctcctcgagg
     3541 cggacgggcg ctttggagct cagccgtcgt acctcgcaca tccgcccgcc tactaccacc
     3601 tcctccgcgg cctcgcgcgc gccacgggcg gaatccccgc cgggacgctg gacgcggcga
     3661 cccgccggct gcgcgctctc tcggcgccgc tcttcgcggc cgccgccgcg ctcttcctgt
     3721 ggctcggctt ccggcgcgcg gcgccgctcc cggcgcatgc tctcttcgcg gcgacgatcg
     3781 cgaccgtgcc gccgctcgct ttcgtcggct ccgccgtgaa caacgacgtc ctcgccttcc
     3841 tcgccggcgg cctcgcgctc ctcggcctcg cgcgcctcct cgaggggcgc gccggcgggg
     3901 cgaccggagc gctcgtcggc gcgggcctcg cgctcgcgct cctcgcgaag gcgacggccg
     3961 gcctcctcgt cctcctcgcc gtggcggcgg ctctcgccct cgcgcggcgc accgtccgct
     4021 gggaacggga tccccgcttc ctcctcgccc tcctctcgtg gctcctcctc ccggcgctcc
     4081 actacgtccc gatcctcctc cgctacggca cgccggtccc ctcgctggac gtcgtcgacc
     4141 cggaggcgtt cgcccgttcg gccttcgtgg acgcgccggg cgcgccgcgc ctcgccgtcg
     4201 tcccgtgggc ggtgaagctc ctgaaggtct tcggctcgac gtggctctcg ctcatcgcgc
     4261 acgtctggct cccggtcggc ccgctcccga cgctcgccgg gccggccctc gccgcgctcc
     4321 tcgcggcgcg cgggctcctg cgacgtcggc gctcggacgg cgccgccgaa gccccgctcg
     4381 acgacctcgt ccgggccggc gccgtcgccc tcgccgggac gctcctcgtc aacctcgcgt
     4441 gggcgcggga cggctatctc gccacggagc gcctcggagg catgcacgcg cggtattacc
     4501 tcccgctctt cccggtcctc gccttcgcgg cggcgcgggg actcgagcgc atcccggcgc
     4561 gcgggctcgc gcgccttacc ggacggggcc tcgtcgccgc cgcggccgtc cttctcctcg
     4621 tcctcttcga cgcgggggtg acggcgcgct acctcgccct cttcccggcg ccctgacgct
     4681 cccgggcgcg cgctagacgc cgtcgcgggc gaggaagcgg gccgaggcgc gggcgacgac
     4741 cttccggtcc tgctccacct cgcctcggac gtagtagagg gggtgggccg tcgtcttctc
     4801 cacgcggccg cggacgacga cgccccgctc gagggcgacg ggcgcgcggt agcggacgtc
     4861 catctcggcc gtcacggcga cgacgccgat tgcgaagagc gcgttcgcca tcgcggcgtc
     4921 gaggagcgcg gcgacgatcc ccccgtggag cgtctccggg tagctctgga agacctcgcg
     4981 gcacgggaag aacgccgcga ccgagccgtc gggctgcacg cggaacctca gcttcagccc
     5041 ggcggggttg tcagggctgc agaggaagca gcgcgggtgc tcggcggcgc ggagggcgtc
     5101 gagccgctcc tggctcggcg tcgggctttc cgacgggttc atctcgtcgg gccccggcgc
     5161 cggagggcct cctcctcggt cttcaggctc tcggctcgcg ccttcagcgc ggtggcgcgg
     5221 gcggcgacct cggcgagggc cgacgcggga tcgggcgagc ccgtcgcgga cttcagcgcg
     5281 cggagcatcg cgagcgcgtc gtcgaagctc ccgagagtct cgcggtggtg ggcggcgcag
     5341 gctgcgggcg gcgagatccc gaggagccgg cggcgcgcct cctccgcctc gcggacgagc
     5401 ccgtcgaggc ccgacgcgtc tccccgcgcg agggccacgg ccatctcgtt cgcgacgctc
     5461 tccgggctcc ccgcgaggcc ggacgcctgg atcgcgtcga cggcgtcgag gtaggccgcg
     5521 atcgcggcac gttccgggtc gtcgggcggc gcgaggtcgg gagcgggcag gggtgtcggg
     5581 aggaacggcg ctcgcagctc gggaccgacc tcgggtgccg cctgcgttgc gggggaggag
     5641 gggagctcca cgtacgcggg ctccgcgtgc gacggcggag ggacgggagc cgtgagggcg
     5701 gccgcgccgg ccgctgcggg ggtcgcggcg aaggccgggg cggctccttc gagccgaggc
     5761 gccgcccgct ccgcgccgcc gttcgcccgc ccgaggaggt acgcgacggc gaggagcgac
     5821 agtccggcga cgccggcgag ggcgcacagg agcctccggt cgaccggcac cgtcgccacg
     5881 cggcccgctc cctaccggag ctccgcgcgg atcgccgcga ggtcggggtc gacggcggcc
     5941 tgcgcggcga gcttcgggtt cagcctcagc gcgcgccggg ccgcctcgag ggcgccggcc
     6001 cggtctcccg ccgcgaggcg gatgaccccg aggtcgtaga cgacctcggg gagcatcgcc
     6061 ggctgcgcct cggcgcgggc ggtcgcctcc tcctgccact tcagcgcctc ggcgtaccgc
     6121 ttctccatga agccggtcca gccgcggttc cagaggtcgt tcacgaccgc catcccgacg
     6181 agccgcgcga gctcgcggaa cgggtcggcg tggtcgtcca cgcgaacgtc gacggcgcgg
     6241 tcggtgaagc cgttgtagcc ccccttcgcg cggcagacga ggagggcggc cgactgccgc
     6301 ccgcggctgt cgccgccggc cgcgtcgccc gcggcgagag cggcgaggag ccgctcggcg
     6361 agcggcttgc cggcgctcgc gaggaacgac cgctccatcg cctcgacgac cgcctcgccg
     6421 acgaggatgt tcccctgcac ggcgtagccc ggccccttcc gcccgccggc ccacgcgttc
     6481 gcgccggagc cggtgaacgt cgccgcgtcg cccgaggccg tcacgaggcc ggcctgccgc
     6541 ctctcgcgcc cttcgtcgcc gcggaggagg accttcagcg cctcctcggc cgaaagtccg
     6601 cgcgccatca ggtcgaggcc gtccggcccg aacgaggtgt tcgcggaggc ctgcgtcgcg
     6661 acggcgccga cgttcccctt cgcccagggg acgaccgtcc cgaccgcgaa gaagcggctc
     6721 gcgacggcga cgccgacctc gcccgtcgcc gggtcggccg ccgcgatcga gaacgtcgac
     6781 gggccgggcg gcgcggccgc gagcgtcccc gtcgcgatga gagcaagcgc gaggaggagg
     6841 gtggggacgc gaggtctctt cgtcatgcgt gtgcgcctcg gggggagtct atcgggccgc
     6901 ctcgagcggc gggacgcgtg cgaagacagt cgagatccgc atcgcggcgc tccgtctcga
     6961 ccgcgcctgc gggacgaacc gggaagaccc ggcttccctt cgaaagtccg cggccccagc
     7021 gtaatctccc gtcgccgcat ggactgcgaa acgcgagcct cccatccgac cgggccgtcg
     7081 ccggagagcg cgagatgacg aaggcccggg tcgtcggcgg agcgctctcc gtcgcgctct
     7141 tcctcctcgc cctcgtcttc ctcgtccgtc atcggaccgt cgcgccccct cccgcgtcgc
     7201 ctgcggagga ggcgggatcg acggcacctg ctgcggcgga gaagatggag gagcgggacg
     7261 cgcagggctt cctccacggc cgggtcacga ccctgaccgg cgcgacgtgg gaagggcggc
     7321 tccgcttcgg aggcgatcag gaggcgttct ggagcgacgc cttcaacggc cggaaggtcg
     7381 agaacgcctg ggtccgccac gtccctcccg accgccttcc ccgcgagcgt cgcggcttct
     7441 ccgtcttcgg gctggagctc ggccgccgcg agcgcccggt cagcttcgtc cggctcttca
     7501 tggcgcgcct cggcgatctc tcgcggatcg aggcgcacgg cgggagggtg cgggtaacgt
     7561 tgaagagcgg caccgtcgtc gacctcgacc ggctcgaggc gagcgacttc gacgacgggg
     7621 tccgtgtgtg ggacccgcgg cgcggcgtgg ccgacctcga ctccgcgcgg atccgcgtca
     7681 tcgagctcct ccccggcccg cgcaccggac gggtccccac gcgcctccag gggacggtcc
     7741 ggaccgcgca gggctcgttc cgcggcgacc tccagtggga ccgggagagc tgcgtcggca
     7801 cggacgagct gccggggcgg accacggagg gcgtccggag ggtccggttc gacgcgatcc
     7861 gctccctcga gcggcgcgaa ggggccgtcc tcctcgtgac gaaccacgac ggccggcagc
     7921 tgtcgctcgc ccgggcgccc gagcgcggct ggggcggccg tggaacgtac gtcgacgacc
     7981 cccgctacgg ccgcgtcctc gtcccgtggg aggccctcga gcgcgtcgac ttcgagccgg
     8041 gcggcagcgg tccttcctac gaggacttcc cgccgggcgc gccgctctcg gggaccgtct
     8101 ccacccgcga cggccgccgc ctctcgggcc gcctcgtcta cgacctcgac gagagcgagg
     8161 tcaccgacac gctcgacgcg ccgtcgcagg gagtcgacta catgctcccg ttcggcctcg
     8221 tcgcctcgat cgagctcccc ggcgcggacg agcgccccgc gccccgcgcc cgggtcaccc
     8281 tccacggcgg ggaggagctg cgcctcgagc tcgccggcga cctcggggag cggaatgccg
     8341 gtctcctcgt cttcgccgcg gaccgccccg ctccggagtt cgtcgcctgg gccgacgtcg
     8401 cgcgtgtcga cctcgaccgt ccgaagagct tcttcccgcc ccccggcggt ctggacgagt
     8461 agctacgcgg agcgcacggc ctcggccacc gggctcaggc ggaggaccgt cggcatccgg
     8521 cgcgtgagcg cgaccggccc cgcgaacgac acgcggcccg accggaccgc ctcctcgaga
     8581 gggagccgcc ccagccagac ctggtagagc gacgcgacgt cggacgagat cgtcacgtcg
     8641 gtctcgaagc ccgggtcgac gtggcacacc gacggaccgc cgggctcgac gacgatccag
     8701 aaccgcctct cgtcgtcgga aaagcggacg tggaagacct ggcgccttcc cgggagctcc
     8761 gacgtgtcga ggcgcgtgtg catccaccag acgaggagcg ccgcgtcgag ctcctcgcgg
     8821 cgcggctctc cgaacgtcca gcgcgcgccc cactcgccga gggcgaagac gatcggccgg
     8881 agctcgcgcc ctgccgcggt gagcaggtat tccccttcga ggcgctccac gaggccggcc
     8941 cgctcgagct ggcggagccg ctgcgagagg aggctccgcg agaggcccgg caggccgcgc
     9001 gccagctcgt tgaaccgggt ggtgccgacg aggaggtcgc gcacgacgag cagcgtccag
     9061 cgctcgccga ggaggtcgag cgcgcgggaa atggggcagt actgtccgta gccggaccgt
     9121 cgttcgctca cgtcgtcacc tcccgggccg ggtgtcggtg cgaattctgg accggtccgg
     9181 tctcgttttt gaactggtcg cgggcccggc gcgttcccat ccttcccgca tgacgacgat
     9241 gcaggacacg acgaccacgg cgaccgactg gctcgccctc gcccgccgga tcggcgagac
     9301 cctcgagccc ggcgtcgccg agcgcgaccg cctcggcgag atcgacctcg cggccttcga
     9361 ggctttccgc gcggagggcg tcaccgcggc gctcgtcccg gccgaggcgg gaggtggcgg
     9421 cgcctcccac gcggagaccg gtcggttcct ccgtgagctc ggccgccacg acggcgcctc
     9481 ggccgtcgcc ctctcgatgc acatgcacct cgtcgcggcg caggtctggc gccaccggca
     9541 cggcctggac gcctccgcgc tcttccggaa ggtcgtcgaa ggccgggccg tcctcgtcag
     9601 caccggcgcg gccgactgga tcggctcgag cgggagcgcg cggaaggccg acggcggctg
     9661 gcgcgtgaac gcccgcaagt cgccggcgag cgggtgcgag gcggggactg tcctcgtgac
     9721 gagcgcccgg tgggagttgg cccccggcgg gccccgcgtc cttcacttcg ccgtcccgat
     9781 gacggcggag ggggtccgca tcgagaagac ctgggacacg ctcggcctcc gcgccacggg
     9841 ctcgcacacc gttgtcctcg aggaggtctt cgtccccgac gccgcggtct cgctcgagag
     9901 gcgggccgac gtctggcacc cggtctggaa cgtcatcctc ggcgcggcga tgccgctcat
     9961 cctcgccgcc tacgccggca tcgccgacgc cgccgtcgcg cgggcgctcg aggcggcgaa
    10021 ggggcggacc gacccgcacg tcctccagct cctcggcgag atgctcaaca cgcacacaac
    10081 ggccgtcgac accgtcgacg cgatgttccg cgacgcggac gacctccggt tcgaggcgac
    10141 cgactccttc gccggccgcg ccctctcgag gaagacggtc gccgccgacg cgctcatcgc
    10201 gaccgtgcgc ctcgcgatcg aggcgacggg cggaggaggg tacgcgcgga gctcggacct
    10261 cgagcgtctc taccgcgacg tccacggcgt cctcttccat cctctcccgc gcgcgcggca
    10321 gacgaccttc agcgggaggg cggccctggg cctcgccccg gtggggtgag gagcgcgccg
    10381 gctccgcccg ggaaggccgg cgcgctaccg cacgaggcgc cgcccgacgt agacctcgta
    10441 ctcccccgcc gccgagtgct cgagcggcac gacgagcgcc tcgaagccgg ccgcggcgag
    10501 cgtctcgagc cagaacgtgc gcgggaagag ccccatctcg agccggtcgt gcgcgacgcg
    10561 cacgtcgccg cctgcgtcgc gcaggaggta ggccatctcg acgacgtacc gctccgcctt
    10621 cgggtcgggc cggaaccgcc attcgaggta gcggaggccc cgcccgccgc cgtcgctccc
    10681 gcccgagtcg ctcccctcct cgaacgtctc cgtcacgcag tcgggctgga acagggcgac
    10741 ccctcccggc gcggtgtggg cgaacgcggt ggcgaccgcc gccgcgaggt cgtcgcgcgt
    10801 cgtcatgtgg gagaccgcgt cgtggaggag gaccgagtcg aagaggcgcc cgagccgaac
    10861 gctccgcatg tcccccgcga cgtgctcgca ctctgggttg aggcgccggc tgaggtcgag
    10921 catcgcgggg gagaggtcga cgagcgtcgc ctccagcccg ggcttcatgt ggaacgcgtt
    10981 gtgcccggcg ccgcagccga ggtcgaggag tgttcggggc ggccgcgcgg cgtgggcctc
    11041 gaagagccgg cggtagaacg ccgcctcctc gacgtagtcc tccacaggcg tcaggagcgg
    11101 gtaccactcg gcgaggtcgc ggtagaggcg cggcgattcg gcttcgcgat cccggtccga
    11161 agagtccatg gccgtcctcc cttcctcccg gtggcgggcc cgatcatcgc gcagcgcccg
    11221 accgtgaccg gtgaaacgcg aaacgccggc atcgctgccg gcgcccgtcg atccgctcac
    11281 cccttctcgg gacggcgggg gaagtgcgat ggctccgcag gtaggatttg aacctacaac
    11341 ccttcggtta acagccgaat gctctgccgt tgagctactg cggaacgttc tcgaaagagc
    11401 ctccgcgagg ccgaaccgcg cggaaggcgg agtctaggaa accggcgccg cagcgtcaag
    11461 cgaggtccgg ggtgggctac cctatcgggc cgaagccgcg tcggggagga aacgagccgt
    11521 ggatcgtggc gggacgggcc gggtcgctgc ggaagggggg cgcttggccc ctcctcgggg
    11581 ccgtttcgct ccgtctccga ccggcccgtt gcacctcgga agcctggttg cggcggtggg
    11641 ttcgtggctc ttcgcgcgga gcgcgggggg cgagtggctc gtccggatgg aggacctcga
    11701 cgggccgcgc gtcgtgccgg gagcggcggg cgagatcctc cgcgcgctcg agcgctacgg
    11761 cctcacgtgg gacggggagg tcgcggtcca gtcgcggcgg acggcgctct acgaggaggc
    11821 gctcgcacgg cttcggaccg cgggccatgc gtacgactgc ggctgcacgc gggcggaggt
    11881 ggcgcgggcg gccggggcgc ccgacgcctc ggacccggtc gacgcggcgg gggccgtcta
    11941 cgcggggacc tgccgggcgg gcctgccgcc ggggcgcgcg ccgcgcgcgg tgaggttccg
    12001 cgcaccggag ggcacggtcg tcttcgagga ccgcgtccgc ggccggatcc aggagaacgt
    12061 cgcgcgtgcc gtcggcgact tcgtcgtgag gcgggccgac gggccgttcg cctaccagct
    12121 cgcggtcgtc gtcgacgacg cggcgcaggg ggtgacggag gtggtccgcg gcggcgacct
    12181 cctctcgtcg acggcgcggc agatcgcgct ccagcgggcg ctcggcctcc cgacgccgtc
    12241 gtacgcgcac ctgccgctcg tcctcggccc tgacgggagg aagctcggca aacgcgacgg
    12301 cgcgcttccg ctcgagacgc tcgacgaggc gcgggtgagg gagacgctcg gcctggcgct
    12361 gcggcttctc ggacaggagc ccccggacgg tccacccgcg gagatgctcg cggaggcgaa
    12421 ggcccggttc gagacgaagc ggatccccgc cggtccggtc cacgtcggcg cgtacggggc
    12481 gtcggcgtga cggccctttc cgcgagcgtc ctcggggccg gtctcctccg gctcgtcctc
    12541 gcggcgatcg ccctttcggc gctcgcgctc gtcgggtacg gcctcttccg ggtcgcgggg
    12601 aggatgatgg ggcggggccg cgctccggct tgcccggaag cgggaccacg tcttccgggc
    12661 ttgcggcggg ccgaagtggc cctgccgctg gcgctctcgg cgggcgcgtt cgccgtcggg
    12721 tggctctcct tcgtcgcggc cctcgtctcg acgtggatcg tccccttcgt ctgggcaggg
    12781 ctcgttctcc tctccttgcc cgcgacgcgg gacctcgccc gggacgccgc gacggtcggc
    12841 cggaggcttc gggcgcttct cctcgcgtcg cccgtcgcgt ccgcgatgct cggcctcgcg
    12901 ctcctcgcgc tcctgccgcc cctgctcctc ccgctctggg actcggacgg cctgcgctac
    12961 caggtcgcgc tcccgaagct cttcctcctc gaggggcgcg tcgtcgcgtg cccgtgggac
    13021 gtccacgccg cgctccccca gctcgtcggc ggcctcctcc tcgcgctcct tcccgtcggc
    13081 ggcggcgagg ccgcgaagct gctccacgcg gcgttcttcg tcgccgccct cgcgacgctc
    13141 gctctccttc tgcaccgcga ccggcggagc cggcgcgccg cggtctacgg gccgctcctc
    13201 ctcgcgatcg ccccggtcgc ggcggtgccg gccaccgcgg cgttcgtcga ccacgcggcg
    13261 ctcttccacc tcgccgtggc ggggctcctc gtcgcgcgcg gcgccccggg cggcgccgcg
    13321 ctccccctcg gcgccgcggt cgcgacgaag acgacggcgg ggccgggggc ggcggcgctc
    13381 ctcctcctcg cgtcggcgac ggcgcggagg ggagagcggt tccgggtgct cgcggcgggc
    13441 gccggggcca tccttctcgc gttcgccccg ttcgggctcc ggaacgtcct cgagacggga
    13501 gacccggtct tcccggtggg ccacgtcctc ctcggtgtgc cggtgcccgg aaccgacccg
    13561 gcgctcgtcc gtcgtgtcct cgagtaccgg cccgatgccg tggcgcccct cggcatcggg
    13621 tggctcccgg gcgagccggg actcgcggcc gacgacgtcg ccgggccggc gctcttcctc
    13681 ggtcttctcg cgctgcccgt gaccctccgg gaccggcggg gccgggtcct cctggccctc
    13741 gcgggggcgt accttctcgt cggcttcgtc gcccagccgc tcgcgcgtct cctgatgccg
    13801 ctcttcctcg ccctcgcggg agcggccgcc ctcgcggccg accgttggct ccgccgggcg
    13861 gcgacaccgg tcgtcgcggc gctcgcggcg gggaccttcg cgttcgcgac gctcccggcg
    13921 ctcgggcctt cggccgccct cgcgcacctc gcggggcggc tctcgcgcga cacgttcctc
    13981 gctgcggagg tgcccggcta ccgggccgcg ctcgcggtca acgcgttgcc gggcggccgg
    14041 gtcatggccc tcgacttccc ggggccgtac tacctggctc gtccgtggat cgccgagggc
    14101 gtcgtgaacg agccgccgct ccgcctctgg ctcgccggtg gagcagacgg cgacggcctc
    14161 ctcgagcgct gccgcgccct gggggtcaca cacctcctcg tcacgcccgg gtggggcggc
    14221 ggcacgagcg cgagcctctt cccgctcgcg cggagccggg aggaggcgaa ggcggtcgtc
    14281 gccttccgct cccgcctcag gctcgtcgcg acggtcgacg gggtggacgt cttcgagctg
    14341 ccacgctgac cggcgcggcg aggaccgaac gggcttccgc gagcttccga tcgaacgcgg
    14401 cgaggtcatt ccgctcgacg agcgcgcgcc aggaggcgag ctcctcatcg aggaggtcga
    14461 acaggccggg cgtcgcgggg ttccgcgcct ggatcggaag ggtcaggtcg ggggcctggt
    14521 ggaggatcgg ggcggcgagg aggcagaggt tccggaagct ggtcgaggcg aggccgtcga
    14581 ggctcttcac cctcacgccc tcgcgcgcga gggtcgatcc cgcgaggagc gagaggaggt
    14641 gggcgaggcc gagcgtccgc gcgacccagg cgtcgtgcgc gccgatcggc agcgtcgcga
    14701 gcttcaggcc ctgccccgag aagaggcgcg cggcggcgcg cgcggcggcg gggacgccgg
    14761 cgtcgcagac gatgaggttc tgccccgcga gcgtgcggac gccggggccg aacatcgggt
    14821 ggaccgaggc gacgcggagg ccgcgccggg ccgccttcgc gtagaggggg aggagcggcc
    14881 ccttcacgct cgccacgtcg aggacgaggc ccggggggga gagggcgagg acctcggcga
    14941 ggacggcggg cgtcgcatcc agggcgaccg agacgacgac ggcgtcggcg cccgcggccg
    15001 tctcgagagg gacggaaggg aacccggcga ggcgccggcc ggggtcgtgg atggcgacgg
    15061 cgtggccggc ggcgcggaac gcgcgcgcga ggaggcgccc catccgcccc gcgccgccga
    15121 cgaggaggac tgtccggagc ggggaggccg gggcgcggcg acgctcggac gccgcgggtg
    15181 ccccgctccg tcggccggcg ccgcgcgcgg gcatcgcgct accgcgcgac gcgcaggaag
    15241 cgcttgccga gcctgacgta ccgggccgcg cccggctcga gcgcgaacgg ctcgccgggg
    15301 ttgtcggcct tcgtgaaggc gacgccgtcc tcggaggtgg cgacggcccc ttccttcacc
    15361 ttctggcgcg cgacgttctt gctcgccgcg aggcccaccg cgacgaggag cgaggcgagg
    15421 tcggcgggag ccggatcggg gagggcgacg gtctcggctt tcaccggccc ctcggccttc
    15481 gcgaagcgcc gctcgaactc cgcctcggcg gtcgcggcgg cctcggcgcc gtggaagtcc
    15541 gtgacgagct ggcgggcgag gtccatcttc acccgcttcg ggtgcttcgc gcccgaggcg
    15601 acgtcggcct tcagggcgtc gatgtcgtgc gggaagaggt cggtcaggag ggtccaccag
    15661 gtccacatca tgtcgtccga gacggacatg accttcccga agatctcgtc gggcgggtcg
    15721 ctcacgccga cgtggttgcc gagcgacttc gacatcttct cgacgccgtc gaggccgacg
    15781 aggagcggcg
//
LOCUS       DAOTEZ010000044        15778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2537_length_15778_cov_5.737200, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000044 DAOTEZ010000000
VERSION     DAOTEZ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15778
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2537_length_15778_cov_5.737200"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..>1674
                     /locus_tag="P5164_03725"
     CDS             93..>1674
                     /locus_tag="P5164_03725"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRY43037.1"
                     /translation="MSSAPRPELDKRFDPQTFEMKWYRLQEESGQFRPEVAPEDARPY
                     TLVIPPPNVTGRLHVGHALGRTLEDVLARWRRMQGRAVLWVPGVDHAGIATQMVVERD
                     LTERTGKTRHDLGREPFLETCREWADARRGDILNQIRRLGCSCDFSREYYTLDAARSK
                     AVRRVFSELYHQGLAYRDSRMVNWCPRCATVLSDLEVNHVESSGQLWSVAYPLEDGSG
                     EVVVATTRPETMLGDTAVAVHPEDERYASIVGKEVRLPLTDRLIPVIADEMVDRKFGT
                     GVVKITPAHDPNDFEAGRRHGLPQVVVIGFDGKMTAEAGAAYAGLERTAARKKVLADL
                     RELGLRRDDKPHTSAVGRCQRCDTVLEPLVSLQWFVKIQPLADKAVAATEAREVTFTP
                     ELWKKTYDEWMKNIRDWCVSRQLWWGHEIPAWYCPDGHVTVPKPGEEADPAACGTCGS
                     RAIERDPDVFDTWFSSWLMPLSVLGWPEKTKDLARFYPTDVMVTGFDILFFWVARMIM
                     AGVWFDGRVPFREVFLHGL"
     assembly_gap    1675..1774
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1775..2926
                     /locus_tag="P5164_03730"
     CDS             <1775..2926
                     /locus_tag="P5164_03730"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRY43038.1"
                     /translation="GVWFDGRVPFREVFLHGLVRDERGQKMSKTKGNVIDPLVMCDEY
                     GADAVRFALAVLSGTGRDLPFGTTRVAASRAFATKVWNAARFAMGMLGEAPVADEPDF
                     AALGRVDRWILDRLATTAKKVNDSLEVFRFDEAANALYAFFWTEFCDGYVEMVKQVLR
                     DETVGEEEKAKTRAVLRHALLDSLALLHPFMPFVSCEIREALNGDGAKLPVLPYPAGR
                     DDWRDPFAVEVVETLRETATRIRNLRAERGMAQTEALDAGLEVPEGPVAEALRSHAPL
                     LSHMARLSSLVVAPKVDLPNPFQDHVGVAGLVVALPKKELSPDEEAKVRKEIASVEKE
                     IEGVSRKLSDESFLARAPQAVVEKAKKQLEELAARRAKLVTNLGEDSGR"
     gene            2923..3717
                     /locus_tag="P5164_03735"
     CDS             2923..3717
                     /locus_tag="P5164_03735"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRY43039.1"
                     /translation="MTGPLGALAPLGGARFGRVANVVLLEETASTNDVARALVEKMIS
                     EDTDLLPTCVVARRQTAGKGRAGRSWSTTGEGAVAVSLVAPWPAGPGRIRVPVTAGIR
                     LARALSAAWGVELRLKWPNDLFCRGKKLAGILVEGRALPEGAGYVVVGVGLNVSTTRA
                     ELDALGLSAATSLALEGVDATLLRGDLPLVAVLSALDETLEGEIDDLPAAFAAVTLHR
                     VGDPVEVRDGDRVVAGRYRGLTADGLLRLETDDGVAELISGDVTGF"
     gene            3725..4522
                     /locus_tag="P5164_03740"
     CDS             3725..4522
                     /locus_tag="P5164_03740"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRY43040.1"
                     /translation="MGLLLALDVGNTNTVLGFFEADSLVATARLTTVRERTSDEHALA
                     LRAVIEGKGRKVGEVDDVIVSSVVPPQDFPIRAACRDAFGIDPLFIAPGVKTGMKILY
                     DQPSEVGADRIVNAVAAVTRYGAPCIVVDFGTATTFDVVSASGDYLGGVICPGPGISA
                     DALFSRAARLSRVDLHRPEKVVGKNTAQSIQSGLYWGVLAQAEGLIARIRAELGEPGM
                     KVVATGGLAPLYASASPDLAVVDSDLTLRGLKILHDRNADARPLRRS"
     gene            4482..5207
                     /locus_tag="P5164_03745"
     CDS             4482..5207
                     /locus_tag="P5164_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43041.1"
                     /translation="MTGTPTPALSGAPDGAEDEASYGTRVEEAFIAERGTPFLLSPKD
                     WRLICAWRESGIPSGTVIRAVREAFERKRARGDLSKISSLSYCANAVEVLWEMERRGL
                     VGAHGGRTETPTDGPDAAPRLASLAEALETAGESALAETDPTIVRDALGAARAALAEL
                     DPASGLESLEESLKGVEKTLVKRLEKALGDAERAALERSVEEEAGDLSALSKEARGRI
                     QRALRARALRRRFGLPALTLLGG"
     gene            5211..6500
                     /locus_tag="P5164_03750"
     CDS             5211..6500
                     /locus_tag="P5164_03750"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43042.1"
                     /translation="MAREVSVERVVPGGSGLARLDGAVVLVAGGLPGDRLLVEPRSDG
                     PRLLRADVLSVLSPGPHRRPAEHVCPLARDGACGGCDWPAVRIESHGPLKEELVRDAL
                     RRIGRVPDAEVPGIRFVASPRSYRLRSRLRWDGRGALGFLGRASSSVADLESCEVVSA
                     ALLQRVPALRESLAAASVPECELQTLEGNDGSPLLGELRFDRALPDPERIAAALHGPL
                     DGVRVLFSGRLAASRGASSLRLEVAGATFRVSVSSFFQGNRFLLDAFLEEARAAARDA
                     ARDGVSRGALDLYAGAGFLTRALLEAGFETDAVESDRSSGADLVANLAAWRSEGLPGR
                     GRAVGRRVEEHLHRERERRDVVFADPPREGLAPEVRKALLRIAPRSLFLVSCDPATFA
                     RDLAALLPRYRVSSLTLLDLFPGTHHVETLARLERRG"
     gene            6497..8893
                     /locus_tag="P5164_03755"
     CDS             6497..8893
                     /locus_tag="P5164_03755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HRY43043.1"
                     /translation="MRRSWSLRAPSPAVPAALAFAAGILLAVELPFPPSPLATLALAL
                     GSGAAVVAGLRGARPLAAGGGLLLVVLLAFARSRPELVVDRESARQTAASAQEDDVFE
                     VRGRLVTYWTRSGSLWSARVDVEEARREGTRVDVPGRLVVSVAGETDPGALAGYGDLV
                     RLRGPLRLPDVGAIPQSPFVLAPEPRLPQKSAGQVEVLDGPRGALGALARLHRAASRR
                     LEAGLEGATSGEREAAALAGALLLGETDRLSPSTVSAFRDGGVAHVLAISGLHVGLLA
                     FGLAAALRPFRPRPATRDAIVLLSTAAFAAFTGGRAPVLRAALMIGLYLGARILGRPT
                     SPGQVFGLSALFLLALDPSWLFDVGFLLTYSAVFGIAALAPLFTRPLRERLPAFLADG
                     LAATLGAELCVFPIQAAVFHVVPFVALLSNLLVVPLASAFLFAAVLLLPLLLAGPVLA
                     SLALAPLELLSGAMLGLLRLLDELGAVRVVPTPSFAVATLLGLLLAAAGVLRRRGPRR
                     AALGAAAALVFLVSARPSSAARPGEARLVALDVGQGDSFLLVTEAGRVLVDGGGTFDA
                     AYDFGRTRLLPRLAALGAVSFDAVVLSHPHPDHARGLVALLRLVPVGRLYLPRGAPRN
                     PFLDEALEAVSGGRPPVEALEAGDGFRAAGIEFEVLHPGPERYPRAPENNGSLVVAAR
                     LGGRRLLLTGDVEARAEADLVGRGGDLSADVLKVAHHGSRTSTTEELLREVRPRIALV
                     SAGRRNRFGHPGREVIERLESSGARVFRTDRHGDVALLFRDGRIFPSRPFAGLPGGIP
                     "
     gene            8890..9819
                     /gene="miaA"
                     /locus_tag="P5164_03760"
     CDS             8890..9819
                     /gene="miaA"
                     /locus_tag="P5164_03760"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRY43044.1"
                     /translation="MTAPRGVLAIVGATATGKSALAVETALRLGGEVISADAFTAYRG
                     IDAGTAKPTLEERRGVPHHLIDLKEPTEPFSAGEFARLAREAAREILSRGRLPILCGG
                     TGFYVRAFFEGLFVGPPRDERLREALRLVEARRGAPWLVRALTLLDPESGARISLRDG
                     ARAIRYLEIAVSTGRRPSELFRERPGERWDGPSVKLLLTLPRPVLYGRIERRFRDSIM
                     APLAGEVRRLLEAGVPVSAPGMAAIGYRETAALLEGLLSPPDWADTVIRQTRRYAKRQ
                     ETWFRSEPDFFSIRADAPDLLEEVVSAARPLFS"
     gene            9841..10080
                     /locus_tag="P5164_03765"
     CDS             9841..10080
                     /locus_tag="P5164_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HRY43045.1"
                     /translation="MINKPPINVQDGFFYQLRKENTLVEVVLLSERTRLGRIRRFDKY
                     AVVLEVDGREEMIYKHAIASVATAAVRPAAPAPAP"
     gene            10317..11432
                     /locus_tag="P5164_03770"
     CDS             10317..11432
                     /locus_tag="P5164_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43046.1"
                     /translation="MAAIRRGDAPAASRALSVRKDGVSPAFRLARAYLDLVEGRRDEA
                     RSFLAEATAAHPAWVAALEAEADLAADEGETSGALEAYRALSRLVPSDARARERAETL
                     RASVAASKRAAAEEALAAGDLDAARRHANSLLQLEPESVAALVLLSRTASAGGRHEDA
                     WIWAREARRKAPSEASVAAFAGDAAAKAGRWAEAATLYEEASASDPASATKAEEARLE
                     FKVQNLPQAARAAAQSPRLTRAQLASLLWWTVPEFREALVPPGTEIAVDVVGRPDQGP
                     LVKAIGLGFLDVSPETHRVGTEAPVSRVELAAALRRVALLAGRGRPPKGCLSAADAAS
                     LADCGILGPTPSRVVTGREALRAMEKAARIGREGGTR"
     gene            11429..12106
                     /locus_tag="P5164_03775"
     CDS             11429..12106
                     /locus_tag="P5164_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRY43047.1"
                     /translation="MNAAPDLFAKVSLFEGLTTADRVALARAATVRTYRRGERIVTQG
                     QQGDSFFVLVKGRVAVSILSPEGREVVLSTLAAGDCFGEMALLDEAPRSATVTAAERS
                     ELAVLTREAFFDLLKGNFVLFRGLLSTFSRRLRHANATIEGLASLDVKGRLARYFRDL
                     ADERGRKAGGGWTVVVRPSQREIADTIGSSRETVSRTMSQLARENLIVPKGKAIYVRL
                     EGESAGG"
     gene            12113..>12573
                     /locus_tag="P5164_03780"
     CDS             12113..>12573
                     /locus_tag="P5164_03780"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRY43048.1"
                     /translation="MELRPWTLPNLLTFARLVAIPFLINAVLEGQHGQAFAIFFAMAV
                     SDFVDGYLARHFGMASPLGALLDPAADKLLLVSTFIVFALKSTPSNIHIPLWLVLLTV
                     ARDVLIVVVALVMALGLGIKSFPPTLLGKANTFAEISTVVAILMNNIDRMPA"
     assembly_gap    12574..12673
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12674..12885
                     /locus_tag="P5164_03785"
     CDS             <12674..12885
                     /locus_tag="P5164_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43049.1"
                     /translation="EISTVVAILMNNIDRMPAWVADACFVATFLLTIASGVHYVFRAS
                     SAIAGAAPGSPPTTGESPPAPPLSS"
     gene            12929..13004
                     /locus_tag="P5164_03790"
     tRNA            12929..13004
                     /locus_tag="P5164_03790"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12962..12964,aa:Gly,seq:ccc)
     gene            complement(13194..13676)
                     /locus_tag="P5164_03795"
     CDS             complement(13194..13676)
                     /locus_tag="P5164_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43050.1"
                     /translation="MKRVQSCAASGITVTVDPNACEAHESLPPFTFRHRVIPAVQELV
                     AEQFGHRVPDLAAICAAVTARSASQLLPGERVPYEVFAGSVLVRLPGEAADPGGGSEV
                     ARAVHAWAGRIHPSGRMEVADLSSRHLGSWLAASRSLSEVRFPRAIWAFEGEVPRAFR
                     "
     gene            complement(13717..15153)
                     /locus_tag="P5164_03800"
     CDS             complement(13717..15153)
                     /locus_tag="P5164_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43051.1"
                     /translation="MKEATFRTRATAAATALLIVAAGCTTSHRPRRTSVVPVSVADGD
                     LHDRARRAAVAASSVERHDGFTVGIVEMDDQGRFWDRRQLRALEAEILGQAGGPEDPG
                     VVLTVFVHGWRHDARVCDANLACFRELLRRISLDQREALAARGGDARPRPVIGVYVGW
                     RGLSKTGWPLEQLSFLDRKNAALRVGAGDTTELLTRLDRLRLVLNGAKRDASRLVVLG
                     HSLGGTVVYEALASIFKSRLAADWPGVDVNGASRVIRGFGDLVVLVNPAFEAERWRPV
                     HELASSYADFSRRQRPVLVVVGSETDSATGTWFPLGQWAATLLERTRDREQRAALRTS
                     IANYEPFVTHRLSRADLPPGEAGPRAPTPRVRGCLCDLPCDATPVDDGHHLLAAPAPP
                     SSDPDGPGAGWIGQPCEPYRRLGPLALECKAPGRRRNPFLVVRAAPDVLHEHGGFFNP
                     YFVSFLRRLLIDATAGTTTPEDLSPTSR"
     gene            15508..>15778
                     /locus_tag="P5164_03805"
     CDS             15508..>15778
                     /locus_tag="P5164_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRY43052.1"
                     /translation="MKTSGLPADSLEMTLQSLRDFAEQRLPVARLLQLDHADEFPVED
                     VRALCDPNTLGIQLVFIPEEYGGFGGSAWDVYRTCELMAAIDLGVA"
BASE COUNT         1945 a         5807 c         5565 g         2261 t
ORIGIN      
        1 cggctcgtcc gcgagaggat cgccgaggtc gagcgggcgg gctgagccgg ccgcgaacct
       61 cgctgcgagg cgtcgtttag actcggcggc ctatgtcatc cgcgccccga cccgagctcg
      121 acaagcgatt cgacccgcag accttcgaga tgaagtggta ccggctccag gaggagtcgg
      181 gccagttccg tccggaggtc gcgccggaag acgcccggcc gtacaccctc gtcatcccgc
      241 cgccgaacgt caccgggcgg ctgcacgtcg ggcacgcgct cggccggacg ctcgaggacg
      301 tcctcgcccg ctggaggcgg atgcaggggc gcgcggtcct ctgggtgccg ggcgtcgacc
      361 acgccggcat cgcgacgcag atggtcgtcg agcgcgacct gacggagagg accgggaaga
      421 cgcgccacga cctcggacgc gagccgttcc tcgagacctg ccgcgagtgg gccgacgccc
      481 gccgcggcga catcctgaac cagatccgcc gcctcggctg ctcctgcgac ttctcgcgcg
      541 agtactacac gctcgacgcg gcgcgctcga aggccgtccg gcgggtcttc agcgagctct
      601 accaccaggg cctcgcctac cgcgacagcc ggatggtgaa ctggtgcccg cgctgcgcga
      661 ccgtgctgtc cgacctcgag gtgaaccacg tcgagtcctc ggggcagctc tggtccgtcg
      721 cctacccgct cgaggacggg agcggggagg tcgtcgtcgc gacgacgcgc ccggagacga
      781 tgctcggcga cacggccgtc gccgtccacc ccgaggacga gcgctacgcg tcgatcgtcg
      841 ggaaagaggt ccgcctcccg ctcaccgacc ggctcatccc ggtcatcgcc gacgagatgg
      901 tcgaccggaa gttcggcacg ggcgtcgtga agatcacccc ggcccacgac ccgaacgact
      961 tcgaggcggg gcgccggcac ggccttccgc aggtcgtcgt catcggcttc gacgggaaga
     1021 tgaccgccga ggcgggcgcg gcctacgccg gcctcgagcg gaccgccgcg cgcaagaagg
     1081 tcctcgcgga cctccgcgag cttgggctcc gccgcgacga caagccgcac acgtcggcgg
     1141 tcggccgctg ccagcggtgc gacaccgtcc tcgagccgct cgtctcgctc cagtggttcg
     1201 tgaagatcca gccgctcgcc gacaaggcgg tcgcggccac cgaggcccgc gaggtcacgt
     1261 tcacccccga gctctggaag aagacgtacg acgagtggat gaagaacatc cgcgactggt
     1321 gcgtctctcg ccagctctgg tgggggcacg agatcccggc ctggtactgc ccggacgggc
     1381 acgtcacggt cccgaagccc ggggaggagg ccgacccggc cgcctgcggc acgtgcggca
     1441 gccgcgcgat cgagcgcgac cccgacgtct tcgacacctg gttctcctcc tggctcatgc
     1501 cgctctcggt cctcggctgg ccggagaaga cgaaggacct cgcgcggttc tacccgacgg
     1561 acgtcatggt caccggcttc gacatcctct tcttctgggt cgcgaggatg atcatggccg
     1621 gcgtctggtt cgacgggcgc gtccccttcc gcgaggtctt cctccacggt ctcgnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggcgtc tggttcgacg ggcgcgtccc
     1801 cttccgcgag gtcttcctcc acggtctcgt ccgcgacgag cgcgggcaga agatgtcgaa
     1861 gacgaagggg aacgtcatcg acccgctcgt catgtgcgac gagtacggcg ccgacgcggt
     1921 ccgcttcgcg ctcgccgtcc tctcggggac cgggcgcgac ctgccgttcg ggacgacgcg
     1981 cgtggcggcc tcgcgggcgt tcgccacgaa ggtctggaac gccgcccgct tcgcgatggg
     2041 gatgctcggc gaggcgcccg tcgccgacga gcccgacttc gccgcgctcg gccgcgtcga
     2101 ccgctggatc ctcgaccgcc tcgcgacgac ggcgaagaaa gtgaacgact cgctcgaggt
     2161 cttccgcttc gacgaggccg cgaacgccct ctacgccttc ttctggaccg agttctgcga
     2221 cggctacgtc gagatggtca agcaggtcct ccgcgacgag acggtcggcg aggaggagaa
     2281 ggcgaagacc cgcgccgtcc tccggcacgc cctcctcgac tcgctcgcgc tgctgcaccc
     2341 cttcatgccg ttcgtctcct gcgagatccg cgaggcgctg aacggcgacg gggcgaagct
     2401 cccggtcctg ccgtaccccg cgggacgcga cgactggcgc gacccgttcg cggtggaggt
     2461 cgtcgagacg ctccgcgaga cggcgacgcg catccggaac ctgcgcgccg agcgcgggat
     2521 ggcgcagacg gaagcgctcg acgccggtct cgaggtgccc gaaggccccg tggccgaagc
     2581 gctccgctcc cacgcgccgc tgctctccca catggcgcgc ctctcgagcc tcgtcgtggc
     2641 gccgaaggtc gaccttccga acccgttcca ggaccacgtc ggcgtcgccg gcctcgtcgt
     2701 ggcgctcccg aagaaggagc tctcgcccga tgaggaggcg aaggtgcgga aggagatcgc
     2761 ctccgtcgag aaggagatcg aaggggtgag ccgcaagctc tccgacgagt cgttcctcgc
     2821 gcgggcgccc caggccgtcg tcgagaaggc gaagaagcag ctcgaggagc tcgccgcgcg
     2881 ccgcgcgaag ctcgtgacga acctcgggga ggactcgggc cggtgacggg cccgctcggc
     2941 gccctcgctc ccctcggggg ggcgcgcttc gggcgcgtcg cgaacgtcgt cctgctcgag
     3001 gagacggcct cgacgaacga cgtggcgcgc gccctggtcg agaagatgat ctccgaggac
     3061 accgacctcc tgccgacctg cgtcgtcgcc cgccggcaga cggcggggaa ggggagggcg
     3121 ggtcggagct ggtcgacgac cggcgagggg gccgtcgcgg tctcgctcgt cgcgccctgg
     3181 cccgcggggc cgggccgcat ccgcgtcccg gtgacggccg gcatccggct cgcacgggcc
     3241 ctttccgcgg cgtggggcgt cgagctgcgg ctgaagtggc cgaacgacct cttctgccgc
     3301 gggaagaagc tcgccgggat cctcgtcgag gggcgggccc ttcccgaagg ggcgggctac
     3361 gtcgtcgttg gcgtcgggct gaacgtctcg acgacgcgcg cggagctgga cgccctcggg
     3421 ctctccgccg cgacgtcgct cgccctggaa ggagtcgacg cgacccttct tcgcggcgac
     3481 ctgccgctcg tcgcggtcct ctccgcgctc gacgagaccc tcgaagggga gatcgacgat
     3541 ctccccgcgg cgttcgccgc ggtgacgctc caccgcgtgg gcgatccggt cgaggtccgc
     3601 gacggcgatc gggtcgtggc ggggcgctac cgggggctga cggcggacgg gctcctccgg
     3661 ctcgagaccg acgacggcgt cgcggagctg atctcgggcg acgtgacggg cttctgagga
     3721 gcggatggga ctcctcctcg cgctcgacgt cgggaacacg aacaccgtcc tcggcttctt
     3781 cgaggcggac tcgctcgtcg cgacggcccg gctgacgacg gtccgggaga ggacctccga
     3841 cgagcacgcg ctcgctctcc gcgcggtcat cgaagggaag gggcggaagg tcggcgaggt
     3901 cgacgacgtc atcgtctcct ccgtcgtccc tccccaggac ttcccgatcc gcgccgcctg
     3961 ccgcgacgcc ttcggcatcg acccgctctt catcgcgccg ggcgtgaaaa cggggatgaa
     4021 gatcctctac gaccagccct ccgaggtggg cgcagatcgg atcgtcaacg ccgtcgccgc
     4081 cgtcacgcgg tacggcgccc cgtgcatcgt cgtcgacttc ggcaccgcga cgacgttcga
     4141 cgtcgtctcc gcctcggggg actacctcgg cggcgtcatc tgcccggggc ccggcatcag
     4201 cgccgacgcc ctcttctccc gcgccgcccg cctctcgcgc gtcgacctcc accggccgga
     4261 gaaggtcgtg ggaaagaaca ccgcgcagag catccagtcg ggcctctact ggggcgtcct
     4321 cgcgcaggcc gaggggctga tcgcccggat ccgcgccgag ctcggcgagc ccgggatgaa
     4381 ggtcgtcgcc accggggggc tcgcgcccct ctacgcgtcc gcgtcgcccg acctcgccgt
     4441 cgtcgattcc gacctcaccc ttcgcgggct gaagatcctc catgaccgga acgccgacgc
     4501 ccgccctctc cggcgctcct gacggcgcgg aggacgaggc ctcctacggc acgcgtgtcg
     4561 aggaggcctt catcgccgag cgcggcacgc cgttcctcct ctcgccgaag gactggcggc
     4621 tcatctgcgc gtggcgcgag agcggcatcc cctccggcac ggtgatccgg gccgttcggg
     4681 aggctttcga gaggaagcgc gctcgcggag acctctcgaa gatctccagc ctctcctact
     4741 gtgcgaacgc cgtcgaggtc ctctgggaga tggagcggcg cggcctcgtc ggcgcgcacg
     4801 gcgggcggac ggaaacgccg acggacggtc ccgacgcggc ccctcgcctc gcctccctcg
     4861 cggaggcgct cgagacggcc ggggagagcg ctcttgcgga gaccgatccg acgatcgttc
     4921 gcgacgcgct gggggcggcg cgggccgcgc tcgccgagct cgaccccgcc tccgggctcg
     4981 aatcgctcga ggagagcctc aagggggtgg agaagactct cgtgaagagg ctcgagaagg
     5041 cgctcggcga cgcggagagg gcggcgctcg agcggtcggt cgaggaggag gcgggagacc
     5101 tctccgccct ctcgaaggag gcgcgcggac gaatccagcg cgcgcttcgg gcgcgcgcgc
     5161 tgcgccggcg gttcggcctt cccgccctga cgctgctcgg aggctgaacg gtggcgcgcg
     5221 aggtctccgt ggagcgcgtc gtcccgggag gaagcggcct cgcgcgcctc gacggcgcgg
     5281 tcgtcctcgt cgccggcggg ctgccgggcg accgcctcct cgtcgagccg cgaagcgacg
     5341 gtccccggct cctccgcgcc gacgtcctct ccgtcctctc gcccgggccg caccgcaggc
     5401 cggcagagca cgtctgtcct ctcgcccggg acggggcgtg cggcgggtgc gactggcccg
     5461 ccgtccgcat cgagtcccac ggcccgctga aggaggagct cgttcgagac gccctccgcc
     5521 ggatcggccg cgtgcccgac gccgaggtcc ccgggattcg cttcgtcgcg tcgccgcgaa
     5581 gctaccgcct ccgaagccgg ctccggtggg acggtcgcgg ggcgctcgga ttcctcggac
     5641 gggcctcctc gagcgtcgcc gacctggaat cgtgcgaggt cgtctcggcg gctctcctcc
     5701 agcgcgtgcc ggccctccgt gagtcgctcg ccgctgcgag cgtccccgag tgcgagctgc
     5761 agacgctcga gggaaacgac ggttctcctc tcctcggaga gctccggttc gacagggccc
     5821 tccccgaccc cgagcggatc gccgcggcgc tgcacggtcc gctggacggc gtccgggtcc
     5881 tcttctccgg tcgtctcgcc gcctcgcgcg gcgcgtcgtc gctccggctc gaggtcgcgg
     5941 gcgcaacgtt ccgggtgtcg gtctcctcgt tcttccaggg gaaccgcttc ctcctcgacg
     6001 ccttcctcga ggaggcgcgg gccgccgcgc gggacgcggc ccgtgacggg gtctcgcgcg
     6061 gagccctcga cctctacgcc ggcgccgggt tcctgacccg ggcgctgctg gaggccggct
     6121 tcgagaccga tgcggtggag tccgaccgct cctccggggc ggacctcgtc gcgaacctcg
     6181 cggcctggag gagcgagggg ctccccggcc gggggcgcgc cgtcggtcgg cgcgtcgagg
     6241 agcacctcca ccgggagcgg gagcggcgcg acgtcgtctt cgccgacccg ccgcgcgagg
     6301 gcctcgctcc ggaggtccgg aaggcgctcc tccggatcgc gccgaggagc ctcttcctcg
     6361 tctcgtgcga ccccgccacg ttcgcaaggg acctcgccgc gctcctcccg cggtaccgcg
     6421 tctcctccct gacgctcctg gacctcttcc ccgggacgca ccacgtcgag acgctcgcga
     6481 ggctcgagag gcgcggatga ggcgttcgtg gtcgctccgg gcgccgtcgc cggcggtccc
     6541 ggcggccctc gccttcgccg ccgggatcct cctcgccgtc gagctgccgt tcccgccctc
     6601 tccgctcgcg acgctcgccc tcgcgctggg ctccggcgcg gccgtcgtcg cggggctgcg
     6661 cggagctcga ccgctcgcgg ccggcggcgg cctccttctc gtggtcctcc tggccttcgc
     6721 ccgctcgcgc cccgagctcg tggtcgaccg ggagtcggcg cggcagaccg ccgcttccgc
     6781 tcaggaggac gacgtcttcg aggtccgggg ccggctcgtc acgtattgga cgcggagcgg
     6841 ctcgctctgg tccgcgcgag tcgacgtcga ggaggcgcgg cgggagggca cgagggtcga
     6901 cgtgcccggt cgcctcgtcg tgagcgtggc gggggagacc gatcccggcg cgctcgccgg
     6961 ctatggcgac ctcgtgcgcc tgcggggccc gcttcgtctg ccggacgtcg gtgcgatccc
     7021 gcagagtccc ttcgtcctcg cccccgagcc gcgccttccg cagaagtccg ccggacaggt
     7081 cgaggtcctc gacggccccc ggggtgcgct gggcgccctc gcgcgcctcc accgggccgc
     7141 gagccggcgg ctcgaggccg gcctcgaagg cgcgaccagc ggcgagcgcg aggccgcggc
     7201 actcgccggg gcgctgctcc tgggcgagac cgaccgcctc tcgccgtcca ccgtctccgc
     7261 gtttcgggac ggcggcgtcg cgcacgtcct cgcgatctcc ggcctccacg tcgggctcct
     7321 ggcgttcggc ctcgccgcgg cgctccgccc tttccggccg cggcccgcca cgcgagatgc
     7381 gatcgtcctc ctctccaccg ccgcgttcgc cgccttcacc ggcggccgcg ctcccgtcct
     7441 gcgcgcggct ctcatgatcg gcctctacct cggggcgcgc atcctcggcc gtccgacgtc
     7501 gcccgggcag gtcttcggcc tttccgctct cttcctcctg gcgctcgacc cctcctggct
     7561 cttcgacgtc ggcttcctcc tcacgtattc cgccgtcttc ggtatcgcgg cgctcgcacc
     7621 tctcttcacg cgtccgctcc gggagcggct cccggccttc ctcgccgacg gcctcgcggc
     7681 gacgctgggc gccgagctct gcgtcttccc gatccaggcc gccgtcttcc acgtcgtccc
     7741 tttcgtcgcg ctcctttcga acctcctcgt cgtgccgctc gcgagcgctt tcctcttcgc
     7801 ggcggtcctc ctcctgccgc tcctcctggc cggaccggtc ctcgcctcgc tcgcgctcgc
     7861 cccgctcgag ctcctctcgg gcgcgatgct cggtctcctc cgtctcctcg acgagctcgg
     7921 ggcggtgcgc gtcgtcccga cgccttcgtt cgccgtggcc acgctcctcg ggctcctcct
     7981 ggccgcggcg ggcgtcctgc ggaggcgcgg ccctcgtcga gccgccctcg gcgcggccgc
     8041 ggccctcgtc ttcctcgtct ccgcccgccc ctcgtccgcc gcgcggcccg gcgaagcgcg
     8101 gctcgtcgcg ctcgacgtcg ggcaggggga ttccttcctt ctcgtcacgg aggcggggcg
     8161 ggtcctcgtc gacggcggag ggacgttcga cgccgcgtac gacttcggcc ggacgcgcct
     8221 cctcccgcgg ctggccgctc tcggagccgt ctcgttcgac gccgtcgtcc tgtcgcaccc
     8281 gcaccccgac cacgcgcggg gtctcgtggc gctgctgcgc ctcgtcccgg tcggccgcct
     8341 ctacctgccg cgcggcgcgc cgcgcaatcc gttcctcgac gaggccctcg aggccgtctc
     8401 ggggggccgc cctccggtcg aggcgctcga ggcgggcgac gggttccggg cggcggggat
     8461 cgagttcgag gtgctccacc cggggccgga gagatatccc cgcgcgccgg agaacaacgg
     8521 ctcgctcgtc gtcgccgcgc ggctgggggg acggcgcctc ctcctgacgg gcgacgtcga
     8581 agcccgcgcg gaggccgacc tcgtcggccg cggcggcgac ctctcggccg acgtcctgaa
     8641 ggttgctcac cacggcagcc gcacctccac gaccgaggag ctcctgcggg aggtccgacc
     8701 caggattgcc ctcgtcagcg ccggtcgccg gaaccgattc ggccatccgg ggcgcgaggt
     8761 gatcgagcgg ctggaatcgt ccggggcgcg cgtcttccgc accgaccggc acggcgacgt
     8821 cgcgctcctc ttccgcgacg ggaggatctt cccctcgcgc ccgttcgcgg gcctccccgg
     8881 agggatcccg tgacggcccc gcgcggggtc ctcgcgatcg tcggggcgac ggcgaccggg
     8941 aagtcggccc tcgccgtcga gacggccctc cgcctcggcg gggaggtcat ctcggccgac
     9001 gcgttcaccg cctaccgggg aatcgacgcg ggaacggcca agccgacgct cgaggagcgg
     9061 cggggcgttc cgcaccacct gatcgacctg aaggagccga ccgagccgtt cagcgcgggg
     9121 gaattcgccc gcctcgcccg cgaggcggcc cgggagatcc tttcgagagg gaggctcccg
     9181 atcctctgcg gagggacggg cttctacgtt cgcgcgttct tcgaagggct cttcgtcggc
     9241 cccccgcggg acgagcgcct gcgcgaggcg ctgcgtctcg tcgaggcgcg ccggggcgcg
     9301 ccgtggctcg ttcgcgcgct cacgctcctc gacccggaaa gcggggcacg gatctcgcta
     9361 agggacggag cgcgtgccat tcgatacctg gaaatcgccg tctcgaccgg acggcggcct
     9421 tcggagctct tccgcgagcg gccgggcgag cggtgggacg ggccgtccgt gaagctcctc
     9481 ctcacgttgc cgcgccccgt cctctatgga agaatcgagc ggaggtttcg agactccatc
     9541 atggccccgc tcgccggcga ggtgaggcgc ctcctcgagg cgggcgtacc ggtgagcgct
     9601 ccgggcatgg ccgccatcgg ataccgcgag acggcggcgc ttctcgaggg ccttctgtcg
     9661 ccccccgact gggccgacac ggtgatacgg cagacgcgcc gctacgcgaa gcggcaggaa
     9721 acgtggttcc ggtccgagcc ggatttcttt tctatccggg ccgacgcgcc cgacctcctc
     9781 gaagaggtgg tctccgcggc tcgcccgctc ttctcctgat cttttcgaag ggagacgtcg
     9841 atgatcaaca agccgccgat caacgtgcag gacgggttct tctaccagct ccgcaaggag
     9901 aacaccctcg tcgaggtggt cctcctctcc gagcggacga ggctgggccg catccggcgc
     9961 ttcgacaagt acgccgtggt cctcgaggtc gacgggcgcg aagagatgat ctacaagcac
    10021 gccatcgcgt ccgtcgcgac ggctgccgtc cgtccggccg cgcccgcgcc ggccccctga
    10081 gggccgtcac cggattgagc ccgaccggtc gacgtccgtc ccggccgccc cgcggcccgc
    10141 tgccgaccgc cggcctcctc gtcgcgcttc ttctcgccgt cgcgggcggc tgcaccccgg
    10201 cgccgccgcc cgcgacggcc gttcccgcgg acaccccctt cgccttccgc gacccgcggg
    10261 agggggcccc ggcgccggcg cccacctccc gggaatcgaa ggctctcgac gcggccgtgg
    10321 cagcgatccg ccggggcgac gccccggcgg cgtcccgcgc gctttcggtc cgcaaggacg
    10381 gggtttcgcc ggcattccga ctcgctcgcg cctacctcga tctcgtcgag gggcggcgcg
    10441 acgaggcgcg gagcttcctc gccgaggcga cggcagcgca tcccgcctgg gtcgcggccc
    10501 tcgaagcgga ggccgacctc gccgccgacg agggagagac gtcgggcgcg ctcgaagcct
    10561 atcgggcgct ctcgcggctg gtcccctccg acgcgcgcgc acgcgagcgg gccgagaccc
    10621 ttcgcgcttc cgtcgccgcc tcgaaacggg ccgccgccga ggaggccctc gccgcggggg
    10681 acctcgacgc ggcccgccgc cacgcgaact cgctgcttca gctcgagccc gagtcggttg
    10741 ccgccctcgt cctcctctcg aggacggcat cggccggtgg ccggcacgag gacgcctgga
    10801 tctgggcgcg cgaggcccgc cggaaggccc cctccgaggc ctccgtggcg gctttcgcgg
    10861 gcgacgcggc cgcaaaggcg gggcggtggg ccgaggcggc gacgctctac gaggaggcct
    10921 ccgcatcgga cccggcctcg gcgacgaagg ccgaggaggc gcgcctcgag ttcaaggtcc
    10981 agaacctgcc gcaggccgcg cgcgcggcgg cgcagtcccc ccggctgacg cgggcgcagc
    11041 tcgcgtctct cctctggtgg accgtccccg agttccggga ggcgctcgtg cccccgggga
    11101 ccgagatcgc cgtcgacgtc gtcggccggc cggaccaggg gccgctcgtc aaggcgatcg
    11161 gcctcggctt cctggacgtc tccccggaga cgcaccgcgt cggaacggag gccccggtca
    11221 gccgtgtcga gctcgccgcc gcgctccggc gggtcgccct cctcgcgggc cggggaaggc
    11281 cgccgaaggg ctgcctctcg gccgcggatg cggcatcgct cgccgactgc gggatactcg
    11341 gtccgacacc gtcccgcgtc gtgacaggcc gagaggccct gagggccatg gagaaagccg
    11401 cccgaatcgg gcgtgaggga ggtacgagat gaacgccgcc cccgacctct tcgcgaaggt
    11461 ttccctcttc gagggattga cgacggccga ccgggtcgcc ctggcgaggg cggcgaccgt
    11521 ccggacctac cgccggggag agcggatcgt cacgcagggc cagcagggcg attccttctt
    11581 cgtcctcgtg aaggggcggg tcgccgtctc catcctctcg ccggaaggac gcgaggtcgt
    11641 cctctccacg ctcgctgccg gcgactgctt cggcgagatg gcgctgctcg acgaggcgcc
    11701 gcgctccgcc acggtgacgg cggccgagcg ctccgagctc gccgtcctga cgcgcgaggc
    11761 gttcttcgac ctcctgaagg ggaacttcgt cctcttccgc ggcctcctct ccacgttctc
    11821 ccgccggctt cgccacgcga acgcgacgat cgagggcctc gcgtccctcg acgtgaaggg
    11881 ccggctcgcg cgctactttc gcgacctcgc ggacgagcgg gggaggaagg cgggcggcgg
    11941 gtggacggtc gtcgtacgcc cgtcgcagcg ggagatcgcc gacacgatcg gctccagccg
    12001 ggagaccgtc tcccggacga tgtcgcagct cgcccgggag aacctgatcg tcccgaaggg
    12061 gaaggccatc tacgtccgcc tcgaagggga gtccgcgggc ggctgaggcg cgatggagct
    12121 ccgtccctgg accctgccga acctgctgac cttcgcgcgg ctcgtcgcga tcccgttcct
    12181 gatcaatgcc gtcctggaag ggcagcacgg acaggccttc gcgatcttct tcgcgatggc
    12241 ggtcagcgac ttcgtcgacg gctacctcgc gcggcatttc ggcatggcct cgcccctcgg
    12301 ggcgctcctc gacccggcgg cggacaagct cctcctcgtc tcgacgttca tcgtcttcgc
    12361 gctgaagtcg acgccctcga acatccacat tccgctctgg ctcgtccttc tgacggtcgc
    12421 ccgggacgtc ctgatcgtcg tcgtcgccct cgtcatggcg ctcggcctcg gcatcaagtc
    12481 gttcccgccg accctgctcg ggaaggcgaa caccttcgcc gagatctcga cggtcgtcgc
    12541 gatcctgatg aacaacatcg accggatgcc ggcnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12661 nnnnnnnnnn nnnccgagat ctcgacggtc gtcgcgatcc tgatgaacaa catcgaccgg
    12721 atgccggcgt gggtcgcgga cgcctgcttc gtggcgacct tcctcctgac gatcgcctcg
    12781 ggagtccact acgtcttccg agcctcgtcc gcgatcgccg gcgccgcccc gggaagcccc
    12841 ccgacgaccg gcgagtcgcc gcccgccccc cccctctcgt cttgacggcg cgcccgggaa
    12901 acggtacaag gtgcgtccca cgcctcgagc gggcgtagct cagttggtag agcgccagct
    12961 tcccaagctg gatgtcgcga gttcaaccct cgtcgcccgc tccagttctt acaccccgga
    13021 cgccgccctt cgagccgccg gacctgagcg ttcgtccggg ccccggggga cccgcgtcag
    13081 ctcgagctgc tgcggaacct ctccaggacc tcgcgaaccg cggccgccac ggaatcctcg
    13141 cgcgcggcct cgaggcctgc ccggacctgc tgcgtggccg ccgcgtcgaa gagttagcgg
    13201 aacgctctcg ggacctcgcc ctcgaaagcc cagatggcgc gcgggaagcg gacctcggac
    13261 agggagcggg aggcggcgag ccacgatccg aggtgccggg acgacagatc cgccacctcc
    13321 attcggcccg acgggtggat gcgccccgcc cacgcgtgga cggctcgggc gacctcgctc
    13381 ccgcctcccg gatcggccgc ctcgcccgga aggcggacga gaacgcttcc cgcgaagacc
    13441 tcgtagggga cccgctctcc gggaaggagc tggctggccg atcgggccgt gaccgcggcg
    13501 cagatggcgg cgaggtcggg gacccggtgg ccgaactgct cggcgacgag ctcctggacg
    13561 gcaggaatga cccggtgacg gaaagtgaag ggcgggagcg attcgtgcgc ctcgcaggcg
    13621 ttcgggtcga cggtgacggt gatcccggag gcggcgcagg actggactcg tttcatcgaa
    13681 gtctccggcg gtgcgagccg aaacgcccga aacgccctat cgactcgtcg gggacaggtc
    13741 ctcgggcgtc gtcgttccgg ccgtcgcgtc gatcaggagg cgccggagga acgagacgaa
    13801 gtacgggttg aagaaacctc cgtgctcgtg caggacgtcg ggcgcggcgc ggacgacgag
    13861 gaacgggttc ctgcgccgtc ccggagcctt gcactcgagc gcgagggggc cgaggcggcg
    13921 gtacggctcg cacggctggc cgatccagcc cgcgccgggc ccgtccggat cggaggaggg
    13981 aggcgccgga gccgcgagca ggtggtgccc gtcgtccacc ggtgtcgcgt cgcacggaag
    14041 gtcgcagagg cagccccgca cgcgaggcgt cggagcgcgt ggcccggcct cgccgggcgg
    14101 gaggtcggcg cgggagaggc ggtgagtcac gaacggctcg tagttcgcga tcgacgtgcg
    14161 tagggccgcg cgctgctccc ggtcgcgcgt ccgctcgagg agcgtcgcgg cccactggcc
    14221 gagcgggaac caggtgcctg tggcggagtc cgtctcggag ccgacgacga cgaggaccgg
    14281 gcgctggcgc ctcgagaagt ccgcgtagct cgaggcgagc tcgtggacgg gcctccatcg
    14341 ttccgcctcg aaggccgggt tcacgaggac gacgaggtcg ccgaatcctc ggatcacgcg
    14401 gctcgcgccg ttcacgtcga cgccgggcca gtccgcggcg agccggctct tgaagatgct
    14461 cgcgagcgcc tcgtagacga ccgtccctcc gaggctgtgc ccgaggacga cgaggcgcga
    14521 agcgtcccgc ttggcgccgt tcaggacgag gcggagccgg tcgaggcggg tcaggagctc
    14581 ggtcgtgtcg ccggctccga cgcggagcgc cgcgttcttc cggtcgagga aggagagctg
    14641 ctcgaggggc cagccggtct tcgagagtcc ccgccagccg acgtagacgc cgatgacggg
    14701 ccgcggacgg gcgtcgcccc cgcgggccgc gagggcttcg cgctggtcga gggagatgcg
    14761 ccggagaagc tcgcggaagc aggcgaggtt cgcgtcgcag acgcgcgcgt catgccgcca
    14821 cccgtgcacg aagaccgtca ggacgacgcc cgggtcctcc ggcccgcccg cctgcccgag
    14881 gatctcggcc tccagggcac ggagctggcg gcggtcccag aaccggccct ggtcgtccat
    14941 ctcgacgatc ccgacggtga agccgtcgtg gcgctcgacg gagctggccg cgacggcggc
    15001 ccgccgggcg cggtcatgga gatcgccgtc cgcgacggat acgggaacga cggaggttcg
    15061 ccggggacgg tgggacgtcg tgcagcccgc ggcgacgatg agaagcgccg tggcggcagc
    15121 ggtcgcgcgg gtccggaacg tcgcctcctt catgcggcga tgatgccgga gccggcccgc
    15181 ctgccggaag cctctgcccg gcggccggcc tgcgtcggcg gacgggccga gaaggaggcg
    15241 ttccacgggt gcggatcgcg aagggagcct agcggccaac gcgctggaac gaggactctt
    15301 agcggcgctg catcgcgcca tttcctagac ctgttctcga tttgtgtccc ggcctcgcgc
    15361 cgggtaaagt cacccggttt cgctgtggct ccctacctcc catcccccta gctccgaagc
    15421 gcttccgagg atccgcagag ggtcctcttc gtttgtccgg cgggccccgc gggggagccc
    15481 gggccgccgg gggaaagaga gaaccgaatg aagacctccg gccttcctgc cgattcgctc
    15541 gagatgaccc tccagagcct gcgcgacttc gcggagcagc ggctcccggt cgcgcgtctc
    15601 ctgcagctcg accacgccga cgagttcccg gtcgaggacg tgcgggcgct ctgcgacccg
    15661 aacacgctcg ggatccagct cgtcttcatc ccggaggagt acggcggctt cggcgggagc
    15721 gcgtgggacg tctaccggac ctgcgagctg atggcggcga tcgacctcgg cgtggcga
//
LOCUS       DAOTEZ010000045        15671 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2557_length_15671_cov_6.250704, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000045 DAOTEZ010000000
VERSION     DAOTEZ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15671
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2557_length_15671_cov_6.250704"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..1057
                     /locus_tag="P5164_03810"
     CDS             86..1057
                     /locus_tag="P5164_03810"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRY43053.1"
                     /translation="MMWKGFQRPNRLEVDEESGNYGRFTAQPLERGWGTTVGNALRRV
                     LLSSIEGAAITAVKIDGVEHEFTAMPGVVEDVTDVILNLKSLAIRMHDDGPRMLVLDA
                     KSKGVVTADAFEDDAQVEILNPEATVATLADGGRLRLEATVTKGRGYVPADRNFDAEA
                     PIGTIPLDSAHSPVRRVNYHVEAARVGQATDYDKLVLEVWTNGTLTPRDAVGLAAVLL
                     REHLAIFVNLETEEGAAASGPEIPQSELDALLDQNVEELELSVRSANCLKNAGIRTLR
                     ELVQKTEKEMLETKNFGRKSLNEIKEILREKGLAFGMKLETPTGAGR"
     gene            1069..1725
                     /gene="rplQ"
                     /locus_tag="P5164_03815"
     CDS             1069..1725
                     /gene="rplQ"
                     /locus_tag="P5164_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRY43054.1"
                     /translation="MQHNRAGRKLGRTSAHRKALFRNQLDSLFLHERIQTTISKAKEL
                     RPLAEKLVTLARRGGLHARRLALRDISQEAAKRLFDEIAPRFATRPGGYTRILKLGQR
                     PGDAAEKAIIEFVDFDFAQRRAEKKAAEKAVSEKKESLLEKARRLVTGKGGDKEAGAK
                     ADEPAESAKADEKKAGDKKVKAPKAAKPAKTAAPKAPKPKAEKKGGSTPRTPRKAGGA
                     "
     gene            complement(1900..2334)
                     /gene="acpS"
                     /locus_tag="P5164_03820"
     CDS             complement(1900..2334)
                     /gene="acpS"
                     /locus_tag="P5164_03820"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016360705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HRY43055.1"
                     /translation="MTTDPRSALLDGAYPHGKAVLGLGVDLCEIARIEAALARHGSRF
                     LEKVCVPGEVRRPLHSRRIGEHVAGLFAAKEAAMKALGTGMQGVAFREVAVAREPGGP
                     PRLVLAGRAREKADALGVVGAHVSITHGTREAVAVVLLLGGR"
     gene            complement(2331..2579)
                     /locus_tag="P5164_03825"
     CDS             complement(2331..2579)
                     /locus_tag="P5164_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43056.1"
                     /translation="MSETRGGRFLQLLLGLYFLEAGLLLVLAPWSAFWLRRVVLPSPA
                     SVEPFLAAPSFRGFVAGLGVLHLVLAARVLLGRPERPA"
     gene            complement(2662..5028)
                     /locus_tag="P5164_03830"
     CDS             complement(2662..5028)
                     /locus_tag="P5164_03830"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRY43057.1"
                     /translation="MTTRILRPALAIAFLLSAVPALAQREGYSYLSWVGSEVTLVSSA
                     EDDSTARPNTPILVGDRISTGSASRAEAILADGNVLRIDVGTDLRFDRLSRTYESEDD
                     RNSIYLERGGIHLEHRWSTSRDEATRIDTGDVTVLFPDKGLLRVETGRRGTEVYVLSG
                     RAEIAARSGRATLRAGQYAYVSGDSELEVDWLGEPRDRFAAFVSDRRSYSADRPSSDD
                     VVAGYEYDYAVADFDRYGSWITVGGSRAWRPTVSVGWRPYTDGYWRWSPVGLTWVSYE
                     PWGWLPYHYGSWSWDVGFGWYWTPGIRYSPAWVYWSYTPSWVGWCPIGYYGSYYHSYY
                     DRSRRNRRGSERWTNAYPHLRGRIDLERIDPRGWSYTSVSRLGSRFDARRDVLGHDAV
                     RFRPGERGLVATTPLRIERGRGGSVTSVVQEAVRRVPRTLEAAPRGGGGLDDLTPVLR
                     REGTLGSTQSEGLRRAWVTAGEDPSYRPVPADQLAAPRRIADSSTTTVTAPSRGGEGG
                     IGASSSPSRTNDATAPARGSSSRPGEGWRDAGARSGETAGTARQDVEPPLRGGFGNRD
                     VRGDDGWRSPGSESRPGSTPSRTFDVPARRAAPPTESSETPARTGTRETAPRSEPWRA
                     SEPSRGADTPARTRDSEPAPAPRRSAPAPRADEGWRGDDTPRSAPAPRTEAPPSRSYE
                     TPSRSYDAPSRSAPAPRYEAPARRSYETPSRGYEAPSRSSSPERSAPAPRYEAPPSRS
                     SSMPRSAPAPQTSAPSRSSSPSSSSSRPSSPPPSRSGDSSRRSASPRG"
     gene            5215..6027
                     /locus_tag="P5164_03835"
     CDS             5215..6027
                     /locus_tag="P5164_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRY43058.1"
                     /translation="MVFEFLKKKLQSLTSDAVAAAGTAPAAGGDYEERRARAEALHEK
                     GETAEAVALLEGLAADLAGEGSFPLAVAVRHQISSWSSGPPATQSAARDAEEMARKRE
                     ETGAVRARDAGRASAPIPEGLERSRFFAEMTREEIAGFIASSVRRTFREGEIVLEQGK
                     AGRDFYIVTRGVLRVETAMGEGRKLRMGVLVAGDVFGEVAFLTGRPRTATVLAETAAE
                     CLEIGGDAWDGALAGLPRVRKVLEEVHNERARFAADAIVEELRRKREAGGKG"
     gene            6028..6543
                     /gene="def"
                     /locus_tag="P5164_03840"
     CDS             6028..6543
                     /gene="def"
                     /locus_tag="P5164_03840"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRY43059.1"
                     /translation="MAIRDIVRYGDERLVAKNEDVTDFEDPALRELIRDLFETGWAAP
                     GLGIAAPQIGVNKRVCVVDLSVGKDPSQALVLINPRVVDAEGLVRDEEGCLSFPDIVE
                     IVERPEAVAVEAFDEKGVRRVLEGADLLARAFCHEIDHLDSRLFIERMSSLKRGFVLR
                     KVLKRQKRGEW"
     gene            complement(6724..7626)
                     /locus_tag="P5164_03845"
     CDS             complement(6724..7626)
                     /locus_tag="P5164_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY43060.1"
                     /translation="MFRPRSSRPLILLVDDDPFQSEVASYIAEELDCDFEFALSGTEA
                     LAKVAQRAPDVVLLDVKMDDLSGYEVCRRIKTLPETADTQVLFVTARTDEEDLLKGFE
                     ALANDYLTKPFSARELKARTKNALRTKHLLDTIGARTRFLELQQEITEKLEAEEAGTE
                     EKRAQVLVTILDRIGPLFGADGVTLHTRRPGRAETRTAVSPSWPDGAPPSYLAVLALS
                     EDPRIEKAPRLPGESRDPSGDWLVVSAPLWVGSELLGTLRLHRRGLPPAPGDSTELEH
                     LVALAGHLARTLHRADLIQELRGH"
     gene            7708..9123
                     /gene="cysS"
                     /locus_tag="P5164_03850"
     CDS             7708..9123
                     /gene="cysS"
                     /locus_tag="P5164_03850"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRY43061.1"
                     /translation="MSLRLFDTAARSLVDFEPLRPGKIGLYTCGPTVYNRVHIGNLRT
                     FVWEDVMCRAFRSLGFDVTQVMNLTDIDDKTIRGAIAEGLPLRDFTDRHIATFFEDLK
                     TLNVIPAAVYPRATDHVPEMVEIVKKLTERGHTYESDGSIWFRIASFPSYGKLSRVDL
                     SSMKRGARVADDEYEKEDVRDFAVWKGAKEGEPESALWETELGKGRPGWHLECSAMSM
                     KYLGETLDIHTGAVDNVFPHHENEVAQSEGATGKTFVRFWLHAEHLIVDGEKMAKSKG
                     NFYTLPDVLARGFSPRAVRYLLLSVPYRQKLNFTFDALRGAVSALERLESLETRLGER
                     AGAAGADGASEAFRGRVEEARRSVRAAWEDDLNSAASLGALFAFVKEANTALEGGQLD
                     PADAAAARALLREVDGVLGVLAPAEDASLEAEVEAKIAARQDARKRRDFAASDAIRDE
                     LAARGILLEDTPQGVRWRRKS"
     gene            9304..10797
                     /locus_tag="P5164_03855"
     CDS             9304..10797
                     /locus_tag="P5164_03855"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HRY43062.1"
                     /translation="MALTADKTLLVASAGRPTNATLAAAAPFLDPRLPAVELRLDALE
                     EAPELPALRAAFAGRTLLATLRSRAEGGRFGGTASEASALLGAALDAGFDLVDVELQL
                     GGAGLLGLPRERVVVSSHAFERTPDDLEERAARLEATGARFAKLVVAAAGLGDALRLL
                     RLQAARGREGRLAAFGMGEAGLVTRALSPYLGASLSFGAALAPEATAPGQLPAADLLD
                     VYGVGRSRRVEALFALLGGRVSHSLSPAIHNARFEELGLSSLYAPVALSSLATEIGPL
                     REACDELGLPLRGASVTVPFKEEAAAVAGAPGEARNTLLFGEDGAVASANTDRDALLA
                     AIPEAPEDGAALVLGAGGMARSAVEALVARGWRVDLAARTAARAAAIAASCGARVVAS
                     PASAGVAYSAIVNATPLGLDVEDSLPCPGTFLGPGVLAVDAPYRPGGTAFARLALRNG
                     SRLVDGFTLLLAQAAGQSALFTGRAATAESLAARLPSRISALFEVSR"
     gene            10794..12422
                     /gene="hisG"
                     /locus_tag="P5164_03860"
     CDS             10794..12422
                     /gene="hisG"
                     /locus_tag="P5164_03860"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRY43063.1"
                     /translation="MSVPSPNLPRGVQALLFESAERRRRLEDSVVRVLADAGFRETVL
                     PVLDFSAPYDGVTAEGDERLYRFVDRGGELLALRADFTPMAARVVAPRLAGLPMPVAL
                     FYRGDVVRDEPSGVGRPREFAQVGAELYGDPSFEADLRMLRTLLAAADGIPAARLVLT
                     LGWAGLLPALLSAVAPGLVARKRSTLDAALADARARRVGKLSERLRAAGATPAAADEV
                     GAALLAGFDPESALFRLPVLAEAARSLAAAARAAREDRPELGVVVDLAGTPAAPYYTG
                     LTFALDAAGGGGSLAAGGRYDGLLGRFGPPAPAVGFCVGLEALASAAEAAEEAPAAPR
                     PFRIATGKGRLLGKTLDLLRAAGVDFPEGDGRRLLLPDRSGRFELLLLKDDDVPTYVA
                     FGGADAGVVGSDRIGESGEEVCEPLELPYGACRLSLIGRAGEEFRPNGHPVRVGTKYR
                     RLAARFFDGRKIPHEVVPLAGSVELAVALRLADVVVDLIETGSTMAAHGLAEIETILP
                     SRATFVVGPRALVERRAEVASLVSRLAAKVESPC"
     gene            12416..13738
                     /gene="hisD"
                     /locus_tag="P5164_03865"
     CDS             12416..13738
                     /gene="hisD"
                     /locus_tag="P5164_03865"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRY43064.1"
                     /translation="MLRVVAANGREGRALLARLSARRSAPDFATLREALPIVEAVLSG
                     GAPALAGFVRDLDGEATPAKGLLVRPAREKEVDPAFAAAFRLARRRLEAYHRRQLPKG
                     FAFRDALGVSFAETPVPHEAVGVYVPGGRAFYPSSLLMGVVPARVAGVKRIVVATPPR
                     AFRGSRELRWALGELGVDAVLLAGGAHGIAGLASVAGCTKVVGPGNRWVAAAKHLVSG
                     VVSVDMPAGPSEVLVLATTGADPALVAADLLAQAEHDPDAVCLLFTDRRDLAVATAKE
                     VERQIRELPTAGTARASLAANGFALVFPTLEAAARAGVPLAAEHVELMGRAAEGYAAR
                     FAATSGAVFVGDGTPTAFGDYLAGPNHVLPTGGAARSFSGLSVRDFLRWGRSVSVPPA
                     AARRLGPAAATLARFEGLEAHARSLDLRAGSPLGRARAERGQARRADR"
     gene            13735..14808
                     /locus_tag="P5164_03870"
     CDS             13735..14808
                     /locus_tag="P5164_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY43065.1"
                     /translation="MTRDPLAAVRPEIRALRAYRFEPGQADVRAKLDFNESPVDVPAP
                     AKAAALAALGARRFALYPEVGAPRLRAALAANAGLPPEQVVPSNGSGEAILAALSVFA
                     GCGGTLLLAPPVFSLYVQMAAIVGAKVATVPLVGDDFRLDEEAFLAAAAKGERTVPLL
                     CSPNNPTGGVVSRDFVRRLCAVAGVVLVDQAYVDFADEADDLLPILPELPNLVIFRTL
                     SKAWSIAGLRVGYAAARPDVADEVGKAILPFSVDVGAEEIALAALAGREEVRARCRAI
                     AVERERVAAALGALGAKVAPSRANFLFFDPPGGDAARVYAGLRARGVLVRDLSAAPPG
                     ALRVSIGTPEENDLFLATLKEVL"
     gene            14805..15401
                     /gene="hisB"
                     /locus_tag="P5164_03875"
     CDS             14805..15401
                     /gene="hisB"
                     /locus_tag="P5164_03875"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HRY43066.1"
                     /translation="MSRTAEVNRTTKETSIALALDVDGMGEARVSTPVGFLTHMLETV
                     AKHARFDLAVDATGDVHVDAHHTVEDVGLALGEALDRALGDRLGIARFGHSYVPLDEA
                     LARSVVDLSGRPYVVFEAPSTPELQFVTKEFPFALVEEFWKSVAFRGKLNLHVDVIRG
                     RNAHHAAEAVFKATARALRQAVALGEATVLSTKGTLTA"
     gene            15398..>15671
                     /locus_tag="P5164_03880"
     CDS             15398..>15671
                     /locus_tag="P5164_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRY43067.1"
                     /translation="MTRVAILDTGVGNVGSVRRAFERLGAEVATTSDPALVAQSSRVV
                     LPGVGAFAPARARLASSGLEEGLRAAVGHGARLLGLCLGFQLLFEAS"
BASE COUNT         2078 a         5666 c         5860 g         2067 t
ORIGIN      
        1 cgcgaggacg tctccctcca ggtcaacgag aagctcatcg tcgagctcta cagcaagtga
       61 cgggcgaccc gaggaggagc gaacgatgat gtggaagggc ttccagcgac cgaaccgcct
      121 cgaggtggac gaggagtcgg ggaactacgg acgcttcacc gcccagccgc tcgagcgcgg
      181 ctgggggacg acggtcggga acgcgctccg gcgcgtcctc ctctcgtcga tcgagggggc
      241 ggcgatcacc gccgtcaaga tcgacggcgt cgagcacgag ttcacggcga tgccgggcgt
      301 cgtcgaggac gtcacggacg tcatcctcaa cctcaagagc ctcgccatcc ggatgcacga
      361 cgacggcccg cgcatgctcg tcctcgacgc gaagtcgaag ggcgtggtga cggccgacgc
      421 gttcgaggac gacgcccagg tcgagatcct caacccggag gcgacggtcg cgacgctggc
      481 cgacggcggc cgcctccggc tcgaggcgac cgtcacgaag gggcggggct acgtccccgc
      541 ggaccgcaac ttcgacgccg aggcgccgat cgggacgatc ccgctcgact ccgcccactc
      601 gccggtccgc cgcgtgaact accacgtcga ggccgcgcgc gtcgggcagg cgaccgacta
      661 cgacaagctc gtcctcgagg tctggacgaa cggcacgctc acgccgcgcg acgcggtggg
      721 gctcgccgcc gtcctcctcc gggagcacct cgcgatcttc gtcaacctcg agacggagga
      781 aggcgcggcc gcgagcggtc ccgagatccc gcagagcgag ctcgacgccc tgctcgacca
      841 gaacgtcgag gagctcgagc tctcggtccg cagcgcgaac tgtctgaaga acgccggcat
      901 ccggacgctt cgggagctcg tgcagaagac cgagaaggag atgctcgaga cgaagaactt
      961 cgggcggaag agcctgaacg agatcaagga gatcctgcgc gagaaggggc tcgccttcgg
     1021 catgaagctg gagaccccga cgggcgcggg acgctgagga cctgaccgat gcagcacaac
     1081 cgggccggac gcaagctggg gcggaccagc gcgcaccgga aagcactctt ccgcaaccag
     1141 ctcgactcgc tcttccttca cgagcggatc cagacgacga tctcgaaggc gaaggagctc
     1201 cggccgctcg ccgagaagct cgtcacgctc gcgcggcgcg gcggcctcca cgcgcgccgc
     1261 ctcgccctgc gggacatcag ccaggaggcc gccaagcggc tcttcgacga gatcgcgccg
     1321 cgcttcgcga cgcgtcccgg cggctacacc cggatcctga agctcggcca gcggccgggc
     1381 gacgcggcgg agaaggcgat catcgagttc gtcgacttcg acttcgcgca gcggcgcgcc
     1441 gagaagaagg ccgccgagaa ggccgtctcc gagaagaagg agtcgctcct cgagaaggcg
     1501 cggcgtctcg tcaccggcaa gggcggcgac aaggaagccg gcgcgaaggc cgacgagccg
     1561 gccgagtccg cgaaggccga cgagaagaag gccggagaca agaaggtgaa ggccccgaag
     1621 gcggccaagc ccgcgaagac ggccgctccg aaggctccga agccgaaggc cgagaagaag
     1681 gggggctcga cgccccggac gccccgcaag gccggcggcg cctaggtccg atgttcccgg
     1741 gagccctccg ccggccggcg gagggctccc gatcgccgcg cccctccctg cggcgatcgt
     1801 cccagaagcc tctgccgggc tcggaacgat gaggccgtgt ccgtcgatcg gcgcgacacg
     1861 gtcgggcggc gcctcgcccg cgtgccggtc gcgccggcct caccggccgc cgaggagcag
     1921 gacgaccgcg acggcctccc tcgtcccgtg cgtgatcgag acgtgggcgc cgacgactcc
     1981 gagcgcgtcg gccttctcgc gtgcccggcc ggccaggacg agccggggag ggccgccggg
     2041 ctcgcgcgcc acggcgacct cgcggaacgc cacgccctgc atcccggtgc cgagcgcctt
     2101 catcgccgcc tccttggccg cgaagaggcc ggcgacgtgc tcgccgatgc gccgggagtg
     2161 gagcgggcgg cggacctcgc ccgggacgca gaccttctcc aggaagcggg agccgtgccg
     2221 cgcgagggcc gcctcgatgc gcgcgatctc gcagaggtcg accccgaggc ccagcacggc
     2281 ctttccgtgg gggtaggcgc cgtcgaggag ggccgaccgg gggtccgtcg tcacgcggga
     2341 cgctccgggc gcccgaggag gactcgcgcc gccaggacga ggtgcaggac tccgagcccg
     2401 gcgacgaagc cccgaaacga aggggccgcg aggaacggct cgaccgaggc gggagacgga
     2461 aggacgaccc gcctcagcca gaacgcgctc cacggggcga ggacgagcag caagccggcc
     2521 tcgaggaaat agaggccgag cagcagctgg aggaagcggc ctccccgggt ctcgctcacg
     2581 cccgcattct agagggggaa gacgagaggg cgccgcccgg acgggcggcg ccctcgggac
     2641 gttttcccga gccggtcccg ctcagccgcg cggcgaggcg ctgcggcggg aggagtcgcc
     2701 ggagcgcgag ggcggcgggc tcgaggggcg agaagaggac gacgacgggg aggaggagcg
     2761 ggacggagcg ctcgtctgag gcgccggcgc cgagcggggc atcgacgacg agcgcgaggg
     2821 cggagcctcg taccgcggag cgggggcgga gcgctccggg gacgaggagc gggagggcgc
     2881 ctcgtagccc cgggagggag tctcgtagga gcgccgggcc ggagcttcgt accggggcgc
     2941 cggggccgag cgggacggtg cgtcgtagct ccgggaggga gtctcgtagg accgggacgg
     3001 gggcgcctcg gtccgcggcg cgggagccga ccggggcgtg tcgtcgccgc gccatccctc
     3061 gtccgcccgc ggcgccgggg cgcttctgcg gggagccggg gcgggctcgg agtcccgggt
     3121 gcgtgcgggc gtgtcggcac cgcgggaagg ctcgctcgca cgccagggct cggagcgggg
     3181 agcggtctcg cgcgtgcccg tccgcgcggg cgtctcggag gattcggtcg gaggcgcggc
     3241 ccgacgggcg gggacgtcga acgtgcggga tggggtcgag ccggggcggg actcgctccc
     3301 gggcgagcgc cagccgtcgt cgccgcggac gtcgcggttc ccgaagcctc cgcgcagcgg
     3361 gggctcgacg tcctgccgag cggttccggc cgtctcgccg gagcgggctc ctgcgtcccg
     3421 ccagccttcg ccggggcggc tcgaggagcc gcgcgcgggg gccgtggcgt cgttcgtccg
     3481 ggaaggcgag ctcgaggcgc cgatgccccc ctcacccccg cgcgagggcg ccgtcacggt
     3541 cgtcgtcgag gaatctgcga tccgccgggg cgcagcgagc tgatcggcgg ggacggggcg
     3601 gtaggaggga tcctcgcccg cggtcaccca cgcccgccga aggccctcgg actgcgtcga
     3661 cccgagggtc ccctcgcgcc gaaggacggg cgtgaggtcg tcgagcccgc cgcctccgcg
     3721 gggagccgct tcgagagtcc tgggaacgcg ccggaccgcc tcctgcacca cggacgtcac
     3781 ggagccgccg cggccacgct cgatccgaag gggtgtcgtg gcgacgagcc ctcgctcgcc
     3841 gggccggaat cggaccgcgt cgtgaccgag gacgtccctg cgggcgtcga aacgcgaccc
     3901 gagccggctg acggaggtgt agctccagcc gcgggggtcg atccgctcga ggtcgatccg
     3961 cccgcgaagg tgcgggtagg cgttcgtcca gcgctccgag ccgcgccggt ttcggcggga
     4021 gcggtcgtag taggagtggt agtagctgcc gtagtagccg atcgggcacc agccgaccca
     4081 ggacggcgtg tagctccagt agacccaggc gggcgaatag cggatgccgg gcgtccagta
     4141 ccagccgaag ccgacgtccc agctccagga gccgtagtga taggggagcc agccccaggg
     4201 ctcgtaggag acccaggtca ggccgaccgg ggaccagcgc cagtagccgt ccgtgtaggg
     4261 gcgccagccg acggagacgg tgggcctcca ggcccgggag ccgcccacgg tgatccacga
     4321 tccgtagcgg tcgaagtcgg cgacggcgta gtcgtactcg tagcccgcga ccacgtcatc
     4381 ggaagacggg cggtcggcgg aataggagcg acggtcggag acgaaggcgg cgaagcggtc
     4441 gcgcggctcg ccgagccagt cgacctcgag ctcggagtct ccggagacgt aggcgtactg
     4501 gccggcccgg agcgtggccc gtccggagcg ggcggcgatc tcggcgcggc cggagaggac
     4561 gtagacctcg gtcccgcggc ggcccgtctc gacccggaga aggcccttgt cggggaagag
     4621 gacggtcacg tcgccggtgt cgatccgggt cgcctcgtcg cgggacgtcg accagcggtg
     4681 ctcgaggtgg atcccgcccc tttcgaggta gatcgagttc cggtcgtcct ccgattcgta
     4741 ggtccgggag agccggtcga atcggaggtc ggtgccgacg tcgatccgca ggacgttgcc
     4801 gtccgcgagg atcgcctcgg cccgggacgc cgagccggtc gagatgcgat cgccgacgag
     4861 gatcggcgtg ttcggccggg ccgtcgagtc gtcctcggcg ctcgagacga gggtgacctc
     4921 ggagccgacc caggagaggt acgagtaccc ctcgcgctgc gcgagggccg ggacggccga
     4981 caggaggaag gcgattgcga gcgcaggtcg aaggattcgg gtggtcatcg gagtcatcct
     5041 cccgtcccgg gtaatgcgac tcgcgtgcca cttcgcgagt ccccccgggg agacgttcgg
     5101 accaacgctc gatttgagct cacggcggga tttccgctcc cgaggcagcg tcctcgggcg
     5161 ggcgcccggc ggaagtgcct tgaccgggac gcccggccgg gccacccttg agacgtggtc
     5221 ttcgaattcc tgaagaagaa gctccaatcg ctcacctctg acgcggtggc ggcggccggg
     5281 accgctcctg cggctggcgg cgactacgaa gagcgccggg cgcgcgccga ggccctgcac
     5341 gagaagggag agacggccga ggccgtcgct ctcctggaag ggctcgccgc ggacctcgcc
     5401 ggcgagggga gctttcccct cgccgtcgcg gtacgccatc agatcagctc gtggagctcc
     5461 ggtcctccgg cgacccagtc ggcggcccgg gatgccgagg agatggcgcg gaagcgggag
     5521 gagaccggtg ccgtccgcgc ccgcgacgcc ggccgggcgt cggcgccgat tcccgagggg
     5581 ctcgagcgat cccgcttctt cgcggaaatg acgagggagg agatcgcggg gttcatcgct
     5641 tcctcggttc ggcggacctt ccgcgagggg gagatcgtcc tcgagcaggg gaaggccggg
     5701 cgggacttct acatcgtcac ccgcggagtc ctccgcgtgg aaacggcgat gggcgaaggg
     5761 cggaagctcc gcatgggggt cctcgtcgcc ggagacgtct tcggggaggt cgccttcctg
     5821 acggggcggc cgcgcacggc gacggtcctc gcggaaacgg cggcggagtg cctcgagatc
     5881 ggcggcgacg cctgggacgg ggctctggcc ggcctcccgc gagtacggaa ggtgctcgag
     5941 gaggtccaca acgagcgggc gcggttcgcg gcggatgcga tcgtcgagga gctgcggcgg
     6001 aagagagagg cgggagggaa gggctgaatg gcgatccggg acatcgtcag gtacggcgac
     6061 gagcgactcg tcgccaagaa cgaggacgtg accgacttcg aggacccggc gctccgggag
     6121 ctgatccgcg acctgttcga gacggggtgg gctgcgcccg gcctcggaat cgccgcgccc
     6181 cagatcgggg tgaacaagcg agtctgcgtc gtcgacctct cggtcggcaa ggacccgtct
     6241 caggcgctcg tcctgatcaa ccctcgcgtc gtcgacgcgg agggactcgt gagggacgag
     6301 gaagggtgcc tctcgtttcc cgacatcgtc gagatcgtcg agcggccgga ggcggtcgcg
     6361 gtggaggcgt tcgacgagaa gggtgtcagg agggttctcg agggagcgga cctcctcgcc
     6421 cgggcgttct gccacgagat cgaccacctc gactcccggc tcttcatcga gaggatgtcg
     6481 agcctgaagc ggggcttcgt ccttcgcaag gtgctgaaga ggcagaagcg cggagagtgg
     6541 taggggaggc gccgaaggcg ccgcggggag gctggtggcg agcctccccg atcaggaccc
     6601 tgaggagggg aggcgccgaa ggcgccgcgg ggaggctggt ggcgagcctc cccgatcagg
     6661 accctgagga ggggaggcgc cgaaggcgcc gcggggaggc tcgtggcgag cctccccgat
     6721 ccatcaatga cctcgcagct cctggatgag gtcggccctg tggagggtcc gcgccaggtg
     6781 cccggccagc gcgacgaggt gctcgagctc ggtcgagtcg ccgggagccg gggggaggcc
     6841 gcggcggtgg aggcgaagcg tcccgagcag ctccgagccg acccacagcg gcgcggagac
     6901 gacgagccag tctcccgagg gatcccggga ctcgcccggg aggcgcgggg ccttctcgat
     6961 gcgaggatcc tcggagagcg cgaggaccgc gaggtaggac gggggcgcgc cgtcgggcca
     7021 ggagggggaa acggcggtcc gggtctcggc ccttccgggc cggcgcgtgt ggagcgtgac
     7081 gccgtcggcc ccgaagagcg gaccgatcct gtcgaggatc gtgaccagga cctgggcccg
     7141 cttctcttcc gtcccggctt cctccgcctc cagcttctcc gtgatctcct gctgcagctc
     7201 gaggaagcgg gtccgcgctc cgatggtgtc gaggaggtgc ttcgtccgga gggcgttctt
     7261 ggtccgggcc ttcagctcgc gcgctgaaaa cggtttcgtc aggtagtcgt tcgcgagcgc
     7321 ctcgaagccc ttgaggaggt cctcctcgtc cgtccgggcg gtcacgaaga ggacctgcgt
     7381 gtccgccgtt tccgggaggg tcttgatgcg gcggcagacc tcgtagccgg agaggtcgtc
     7441 catcttcacg tcgaggagga cgacgtccgg ggcccgctgg gcgaccttcg cgagagcctc
     7501 cgtgccggag agcgcgaact cgaagtcgca gtcgagctcc tcggcgatgt aggaggcgac
     7561 ctccgactgg aagggatcgt cgtcgacgag gaggatgagc gggcgggagg agcgtgggcg
     7621 gaacatcgga acgggcttta tccctcgcgc gggcggggtc gtcaaggaga ttgcgatggg
     7681 cccggtccgc gcggagaatc cgggaccgtg agcctgcgcc ttttcgatac tgcggcccgt
     7741 tccctggtcg atttcgagcc actccgcccc ggcaagatcg gcctctacac ctgcggcccg
     7801 acggtctaca accgggtcca catcgggaac ctccggacgt tcgtctggga ggacgtgatg
     7861 tgccgggcct tccggagcct cggcttcgac gtgacccagg tgatgaacct gacggacatc
     7921 gacgacaaga cgatccgcgg cgcgatcgcc gaggggctcc cgctccgcga tttcaccgac
     7981 cggcacatcg cgacgttctt cgaggacctg aagacgctca acgtgatccc ggccgcggtc
     8041 tacccgcgcg cgacggacca cgtccccgag atggtcgaga tcgtgaagaa gctgacggag
     8101 cgcgggcaca cctacgagag cgacggctcg atctggttcc ggatcgcctc gttcccctcg
     8161 tacgggaagc tctcgcgcgt cgacctctcc tcgatgaagc gcggcgcgcg cgtcgccgac
     8221 gacgagtacg agaaggagga cgtccgagac ttcgccgtct ggaagggggc gaaggaggga
     8281 gagcccgagt cggccctctg ggagaccgag ctcgggaagg ggcggcccgg ctggcacctc
     8341 gagtgctcgg cgatgtcgat gaagtacctc ggcgagacgc tcgacatcca caccggggcc
     8401 gtcgacaacg tcttcccgca ccacgagaac gaggtcgcgc agagcgaagg agcgacgggc
     8461 aagaccttcg tccgcttctg gctccacgcc gagcacctca tcgtcgacgg cgagaagatg
     8521 gcgaagtcga aggggaactt ctacacgctt cccgacgtcc tcgcccgcgg cttctcgccg
     8581 cgcgcggtcc gctacctcct cctgtcggtg ccgtaccgcc agaagctgaa cttcacgttc
     8641 gacgccctgc gcggcgccgt ctcggccctc gagcggctcg agtcgctcga gacgcggctc
     8701 ggcgagcggg ccggcgcggc cggcgcggac ggcgcctccg aggcgttccg cgggcgcgtc
     8761 gaggaggctc gacgctccgt gcgggccgcg tgggaggacg acctcaacag cgcggcctcc
     8821 ctcggcgccc tcttcgcgtt cgtgaaagag gcgaacaccg cgctcgaggg ggggcagctc
     8881 gacccggccg acgcggcggc ggcgcgcgcc ctcctccggg aggtcgacgg cgtcctcggc
     8941 gtcctcgccc cggcggagga cgcctcgctc gaggccgagg tcgaggcgaa gatcgcggcc
     9001 cggcaggacg cccggaagcg gcgcgacttc gccgcctccg acgcgatccg cgacgagctc
     9061 gccgcccgcg ggatcctcct cgaggacacc ccgcaggggg tccgctggcg gcggaagagt
     9121 tgacagggtc cggggccttc ccccactatc cgcccccgat gacgttcgcc ccggtcctct
     9181 ggcactggca tcactcatag cgacggcgag cgccacacgc tcgtcggacg tgagtcgtcc
     9241 gagggcgcgg ggtagagaag aagaaccgca gagaccccct cggacgaccg agggggtttc
     9301 gttttggctc tcaccgcgga taagacgctc ctcgtcgcct cggccggccg gccgacgaac
     9361 gcgacgctcg cggcggcggc gccgttcctc gacccgcgcc tccccgcggt cgagctgagg
     9421 ctcgacgcgc tcgaggaggc gccggagctt ccggccctgc gcgccgcctt cgccgggcgg
     9481 acgctcctcg cgacgctccg gtcccgcgcc gagggagggc ggttcggcgg gacggcctcc
     9541 gaggcctccg ccctccttgg cgccgcgctc gacgccgggt tcgacctggt cgacgtcgag
     9601 ctccagctcg gcggcgccgg ccttctcggc ctcccgcggg agagggtcgt cgtctcgtcg
     9661 cacgccttcg agcggacgcc ggacgacctc gaggagcgcg ccgcgcgcct cgaggcgacg
     9721 ggcgcccgct tcgccaagct cgtcgtcgcg gcggcgggac tcggcgacgc gctgcggctc
     9781 ctgcggctcc aggccgcgcg cgggcgagag gggcgcctcg cggcgttcgg catgggcgaa
     9841 gccgggctcg tcacgcgcgc cctctcgccg tacctcggcg cctccctctc cttcggcgcg
     9901 gccctcgcgc ccgaggcgac ggcgccggga cagcttccgg ccgccgacct cctcgacgtc
     9961 tacggcgtcg gccgctcccg ccgcgtcgag gctctcttcg ccctcctcgg cggtcgggtc
    10021 tcccactccc tctccccggc gatccacaac gcccggttcg aggagctcgg gctctcgtcc
    10081 ctctacgctc cggtcgccct ctcctcgctc gcgacggaga tcggaccgct gcgcgaggcg
    10141 tgcgacgagc tcggccttcc gctccgcggc gcctctgtga ccgtgccgtt caaggaagag
    10201 gccgccgcgg tcgcgggcgc gccgggcgag gcgcgcaaca cgctcctctt cggcgaggac
    10261 ggcgcggtcg cgtccgcgaa caccgaccga gacgcgctcc tcgccgcgat ccccgaggcg
    10321 cccgaggacg gcgccgcgct cgtcctcggc gcgggcggca tggcgcgctc ggccgtcgag
    10381 gcgctcgtcg cgcggggctg gcgcgtcgac ctcgcggccc ggacggccgc gcgcgcggcg
    10441 gcgatcgccg cgtcctgcgg ggcgcgggtc gtcgcgagcc cggcgagcgc cggcgtcgcc
    10501 tattcggcca tcgtcaacgc gaccccgctc ggcctcgacg tcgaggactc gctcccgtgc
    10561 cctggcacgt tcctcggccc gggcgtcctc gcggtcgacg ccccctatcg cccgggaggg
    10621 acggccttcg cgcggctcgc gctccgaaac ggctcccgcc tcgtcgacgg cttcacgctc
    10681 ctcctcgcgc aggccgcggg gcagtcggcg ctgttcacgg gacgcgcggc gaccgcggaa
    10741 tcgctcgccg cgcgtctccc ttcacgcatc tccgccctct tcgaggtctc ccgatgagcg
    10801 ttccctcccc gaacctgccg cggggcgtcc aggccctcct cttcgaatcc gccgaacgcc
    10861 gccgaaggct cgaggactcc gtcgtccgcg tcctcgccga cgccggtttc cgcgagacgg
    10921 tcctgccggt cctcgacttc tccgccccgt acgacggtgt cacggccgag ggggacgagc
    10981 ggctctaccg cttcgtcgac cgcgggggag agctcctcgc cctccgcgcc gacttcaccc
    11041 cgatggcggc gcgcgtcgtc gcgccgcgcc tcgcggggct cccgatgccg gtcgcgctct
    11101 tctaccgcgg cgacgtcgtc cgcgacgagc cgtcgggcgt cggccggcct cgcgagttcg
    11161 ctcaggtcgg ggccgagctc tacggcgacc cctcgttcga ggccgacctg cggatgctcc
    11221 ggacgctcct cgccgccgcg gacgggattc ccgcggcgcg cctggtcctg acgctcggct
    11281 gggcgggcct cctgccggcg ctcctctccg ccgtcgcccc gggcctcgtc gcccgcaagc
    11341 ggagcacgct cgacgcggcc ctcgccgacg cccgggcgcg ccgcgtcgga aagctctccg
    11401 agaggcttcg ggcggcgggc gcgaccccgg ccgcggccga cgaggtcggc gcggccctcc
    11461 tcgcggggtt cgacccggag tcggctctct tccgcctccc cgtgctcgcc gaggcggcgc
    11521 gctccctcgc ggccgccgcg cgggccgcgc gggaggaccg gccggagctc ggggtcgtcg
    11581 tcgatctcgc cgggaccccc gccgcgccct actacacagg cctgacgttc gcgctcgacg
    11641 cggcgggagg gggcggcagc ctcgccgccg gcggccgcta cgacggcctc ctcggccgct
    11701 tcgggccgcc ggccccggcc gtaggcttct gcgtcggcct cgaggcgctc gcctcggcgg
    11761 cggaggcggc ggaggaggcg ccggccgcgc cccgcccgtt ccggatcgcg accgggaagg
    11821 ggcgcctcct cgggaagacg ctcgatctcc tccgggccgc gggggtcgac ttccccgaag
    11881 gggacggccg gcggcttctc cttcccgacc ggagcggccg cttcgagctc ctcctcctca
    11941 aggacgacga cgtccctacg tacgtcgcgt tcggcggcgc cgacgcgggc gtcgtcggga
    12001 gcgaccggat cggggagtcg ggcgaggagg tctgcgagcc gctcgagctg ccgtacggcg
    12061 cctgccgcct ctcgcttatc gggcgcgcgg gagaggagtt ccgtccgaac gggcaccccg
    12121 tccgcgtcgg gacgaagtac cggaggctcg cggcgcgctt cttcgacggc cggaagatcc
    12181 cgcacgaggt cgtcccgctc gccggctccg tcgagctcgc ggtcgcgctg cggctcgccg
    12241 acgtcgtcgt cgacctgatc gagaccggct cgacgatggc ggcgcacggc ctcgccgaga
    12301 tcgagacgat ccttccgagc cgcgcgacgt tcgtcgtcgg gccgcgggcg ctcgtcgagc
    12361 gccgcgccga ggtcgcctcg ctcgtctcgc gcctcgcggc gaaggtggag agtccgtgct
    12421 gagggtcgtc gccgcgaacg gacgcgaggg gagggcgctc ctcgcgcgcc tctccgcccg
    12481 ccgctccgcg cccgacttcg cgacgctccg cgaggcgctg ccgatcgtcg aggcggtcct
    12541 ctcgggcggc gcgcccgcgc tcgccggatt cgtccgcgac ctcgacggcg aggcgacccc
    12601 ggcgaagggg ctcctcgtcc gtccggcccg cgagaaggag gtcgatccgg cgttcgccgc
    12661 ggccttccgc ctcgcgcgga ggaggctcga ggcgtatcac cgccggcagc tgccgaaggg
    12721 cttcgccttc cgcgacgccc tcggcgtctc gttcgccgag acgccggtgc cgcacgaggc
    12781 cgtcggcgtc tacgtcccgg gcgggcgcgc gttctacccg tcgtcgctcc tgatgggcgt
    12841 cgtcccggcg cgcgttgcgg gcgtgaagcg gatcgtggtc gcgacgccgc cgcgcgcgtt
    12901 ccgcgggagc cgcgagctgc gctgggcgct cggggagctc ggcgtcgacg ccgtcctcct
    12961 cgcggggggc gcgcacggaa tcgccgggct cgcctccgtc gccggctgca cgaaggtcgt
    13021 cggcccgggg aaccgctggg tcgccgcggc caagcacctc gtctccggcg tcgtctccgt
    13081 cgacatgccc gcgggaccgt ccgaggtcct cgtcctcgcg acgacgggtg ccgacccggc
    13141 gctcgtggcg gccgacctcc tcgcccaggc cgagcacgac cccgacgccg tctgcctcct
    13201 cttcaccgac cggcgcgacc tcgccgtcgc gacggcgaag gaggtcgagc ggcagattcg
    13261 ggagctcccg acggccggga cggcgagggc ctctctcgcg gcgaacggct tcgccctcgt
    13321 ctttccgacg ctcgaagcgg ctgcccgtgc cggagtcccc ctcgcggccg agcacgtcga
    13381 gctgatgggg cgggccgccg aggggtacgc ggcccggttc gccgcgacct ctggcgccgt
    13441 cttcgtcggc gacggcacgc cgaccgcctt cggcgactac ctcgccggcc cgaaccacgt
    13501 tctgccgacg ggcggcgcgg cacgcagctt ctcggggctc tccgtccgcg acttcctccg
    13561 ctggggacgg agcgtctccg tcccgcccgc cgccgcgagg cgcctcggtc ccgccgcggc
    13621 gacgctcgcg cgcttcgagg ggctcgaggc gcacgcgcgc tcgctcgacc tgagggccgg
    13681 cagccccctc ggaagagcgc gcgccgaacg ggggcaggct cggagggccg atcgatgacc
    13741 cgcgaccctc tcgcagccgt gcggcccgag atccgcgccc tccgcgctta tcgcttcgag
    13801 ccgggccagg cggacgtccg cgcgaagctc gatttcaacg agagccccgt cgacgtcccg
    13861 gcgccagcga aggccgccgc gctcgcggcg ctcggcgccc ggcgctttgc cctctacccg
    13921 gaggtcgggg cgccccggct gcgcgccgcg ctcgccgcga acgctggcct ccctccggag
    13981 caggtcgtcc cctcgaacgg ctccggggag gcgatcctcg ccgcgctctc cgtcttcgcc
    14041 ggctgcggcg ggacgctcct cctcgccccg cccgtcttct cgctctacgt ccagatggcg
    14101 gcgatcgtcg gggcgaaggt cgcgaccgtc ccgctcgtcg gagacgattt ccgcctcgac
    14161 gaggaggcct tcctcgctgc cgctgcgaag ggggagagga ccgtcccgct cctctgctcg
    14221 ccgaacaacc cgacgggcgg cgtcgtctcg cgcgacttcg tccgtcgcct ctgcgcggtc
    14281 gccggcgtcg tcctcgtcga ccaggcctac gtcgacttcg ccgacgaggc ggacgacctc
    14341 ctcccgatcc tccccgagct tccgaacctc gtgatcttcc ggacgctctc gaaggcgtgg
    14401 tcgatcgccg gcctccgggt cggctacgcg gccgcgaggc ccgacgtggc ggacgaggtc
    14461 ggcaaggcga tcctcccctt cagcgtcgac gtcggcgccg aggagatcgc cctcgcggcc
    14521 cttgcggggc gcgaagaggt tcgcgcccgg tgccgggcga tcgccgtgga gcgcgagcgg
    14581 gtcgccgcgg cgcttggcgc gctcggcgcg aaagtcgcgc cgtcgcgcgc gaacttcctc
    14641 ttcttcgacc cgccgggcgg agacgccgcg cgcgtctacg ccggccttcg cgcccgcggg
    14701 gtcctcgtcc gcgacctgtc ggccgccccg ccgggcgcgc tgcgcgtctc gatcgggacg
    14761 ccggaggaga acgacctctt cctcgcgacc ctgaaggagg tcctgtgagc cgcaccgccg
    14821 aggtgaaccg gacgacgaag gagacgtcga tcgccctcgc gctcgacgtc gacggaatgg
    14881 gcgaggcgcg ggtctcgact ccggtcgggt tcctgacgca catgctcgag accgtggcga
    14941 agcacgcccg cttcgacctc gccgtggacg cgacgggaga cgtccacgtc gacgcgcacc
    15001 acacggtcga ggacgtcggc ctcgctctcg gcgaggcgct cgacagggcg ctcggcgacc
    15061 gcctggggat cgcccgcttc gggcactctt acgtgcctct cgacgaggcc ctcgcccgaa
    15121 gcgtcgtcga cctctcgggg cgcccctacg tcgtcttcga ggccccctcg acgccggagc
    15181 tccagttcgt gacgaaggag ttcccgttcg ccctcgtcga ggagttctgg aagtcggtcg
    15241 cgttccgcgg gaagctgaac ctccacgtcg acgtcattcg cggccggaac gcccaccacg
    15301 ccgcagaggc cgtcttcaag gcgacggccc gcgccctgcg ccaggccgtc gcgctcggag
    15361 aggcgacggt cctctcgacg aaggggacgc tcacggcatg acgcgcgtcg cgatcctcga
    15421 caccggcgtc gggaacgtcg gctcggtccg ccgggcgttc gagcggctcg gagcggaggt
    15481 cgcgacgacc tccgaccccg ccctcgtcgc gcagtcgtcc cgtgtcgtcc tcccgggcgt
    15541 cggcgccttc gccccggccc gtgcgcggct cgcgtcgtcg ggcctcgagg aagggctccg
    15601 cgccgccgtc ggccatggcg cgcgcctcct cggcctctgc ctcggcttcc agctcctctt
    15661 cgaggcgagc g
//
LOCUS       DAOTEZ010000046        15659 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2562_length_15659_cov_6.302294, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000046 DAOTEZ010000000
VERSION     DAOTEZ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15659
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2562_length_15659_cov_6.302294"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="P5164_03885"
     CDS             complement(<1..937)
                     /locus_tag="P5164_03885"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY43068.1"
                     /translation="MESSTGTHSPSRLPRTVVALGAVSFLTDASSEMIFPLLPLLLAS
                     LPGAPAAALGVIEGIAEATAAAVKVVSGRLSDRAVRRKPLVVAGYSLSSAVRPLVALA
                     SIWPHVLLVRFLDRIGKGVRSAPRDALIAEVTPPDRRGAAYGLHRAMDNAGAVAGPLL
                     AAALLGLLALPLRTVLALSAVPAALALLVLVLGVRETSRPAAAAAVVRTPAAPLPPAF
                     RRAVAAVALFTLSASSDTFLLLKAREVGISAAGLPLVWAFSNAIRAALGTWGGGLSDR
                     HGRKRVLLAAWTLYAACYALFGFVKTPLALLVVLAV"
     gene            924..1673
                     /gene="recO"
                     /locus_tag="P5164_03890"
     CDS             924..1673
                     /gene="recO"
                     /locus_tag="P5164_03890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRY43069.1"
                     /translation="MLLSTTAFILRVDALSEKDVAATLLTRDAGVLRAAFRGARRRFR
                     RGALLELLTEVSVTLFRREGAELVRGDGLELVKSAFGLASRPETAMLLPYVAESLSTF
                     LPEAEAAPEAYRLARHVLDALEEGAPPTLAARYFETWLLRLAGLLPGGTECASCGRSL
                     ASGGARFDPELPGLVGDECAPPGTVPVSGAALRLLADVRRSRLDDLARRGAGDPESLR
                     ALDALLREVRRRFLGHELKSHRFLDSLSGGP"
     gene            1741..2655
                     /gene="glyQ"
                     /locus_tag="P5164_03895"
     CDS             1741..2655
                     /gene="glyQ"
                     /locus_tag="P5164_03895"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HRY43070.1"
                     /translation="MDFQTLILKLSEYWASRGCILEQPYDTEVGAGTMHPATFLRVLG
                     PEPWRCAYVQPSRRPADGRYGENPFRLVKHYQFQVVIKPSPADIQDLYVDSLRAIGIE
                     PAEHDLRFEEDNWESPTLGAWGVGWQVLLDGMEITQFTYFQQAGGIDLAPITAEITYG
                     LERLTMFLTRTRNVMDIEWAPGVPYGEVRRQDEFEVSKYGFEVADTDLLRHLFERLEA
                     ESGRCLAAGLVVPAYETALKCSHAFNLLDARGAVSATERVGVIKRVRDLAVGCAKAWV
                     AARESLGHPLLPKETPRPEAAEAGNGEA"
     gene            2657..4726
                     /gene="glyS"
                     /locus_tag="P5164_03900"
     CDS             2657..4726
                     /gene="glyS"
                     /locus_tag="P5164_03900"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HRY43071.1"
                     /translation="MSEFLLEILAEEIPAGVLSQARNDLLRGLADALAAERIDGTFFA
                     HSTSRRLVLFSRDLPEVQEDHEVEAIGPSVKVAFDAEGRPTRAAEGFARGQGVAVEAL
                     RVVATPKGEYVSVRKVVEGRFTSEILGEVVPTVVAKMSFPRMMRWGDGRDTWIRPVHS
                     VVCLLDGLVVPVTLFGVESGRTTFGHRTLAPGRIVVVGVDDYLAKLRAAHVEPDYGVR
                     RRTLLEKAEALASEAGCTPVEDSALLDSLAHLVEWPGLVRGSFEAGYLELPEEILVTA
                     MREHQKVLPTRDASGALAPHFLAVADHVGDPMGHIVRGNEWVLNARFADARFFWDEDG
                     RVRLEDRVPRLARLSFQEKLGDTLRKAERVEALASRLAGDLGLGESAGEAARAARLAR
                     ADLVTDMVREFPDLQGVVGGLYARRDGEGEAVCRAVYDHYRPAGVDDALPAEPVGRVV
                     ALADRLDTLAGFFALGLVPTGSRDPYALRRAALAVVRILLEGGHRLELPSAIAAARRL
                     HEVSGASEEEVVSTLHPFLLERLRFLLERRGHRHDEIEAVLATPCPDVTDAAERVAAV
                     SRVRRQPDFGALAASFKRVQNILAQAGVTSGGEIDPSLMSDDAERQLASDFFQARGIL
                     DELIGARRYDEALSVMASLGPVLDRFFVDVMVLVEDEAVRRNRLSLLAAMRDQFFRVA
                     RLSEIQE"
     gene            4844..5413
                     /locus_tag="P5164_03905"
     CDS             4844..5413
                     /locus_tag="P5164_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HRY43072.1"
                     /translation="MRTTRRAVALSAAFAFVLSAAPVEARELAGASMPDTLSVAGKTL
                     KLNGMGLRKKAVFKVYVGGLYLEAPSSDAAAILAADAPKAVRMHFLRSVDRGKLVETF
                     REGFAANAGAKAQAQKAGIEKLLATVTDVKDGSVTTYVYAPGAGTVVNRDGKDVATIE
                     GKEFAEVLFSLWLGPKPPSEDLRSGMLGR"
     gene            5464..7563
                     /locus_tag="P5164_03910"
     CDS             5464..7563
                     /locus_tag="P5164_03910"
                     /inference="COORDINATES: protein motif:HMM:NF015414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HRY43073.1"
                     /translation="MNLNELSSLLSEKKFDVVENAVKDALLDAEENAELLAAAFRGLA
                     RASGQKGRLQGLSTLADATLRGPGTSPAAARLRWGLLKEAVRAGATPSSPEGFHKLFE
                     EAIRAAYPESASLTTLLGRFRFLESKEPADGLARVERAEKWLPFEVGRCFFMAVRGAG
                     KVVETNFALDAVRVDFEKSAGVSIPIGVAAKSLTPLPEGHFLHEKLTAREALAESVLA
                     DPSEGLRRLLDSFGTALPVSEVKEAMAGLVPEERWTSWWTAARKSPHVVLHGSGKNAS
                     VEWTRTSQAADAAILAKFGKAHTPESRLDFFRKNAKRDAALAAKMGARLAEDARALRD
                     ANPPLAYEIAVAIEKVEGAALGFAPEDLVPEAPLPMLGRIGDRLARERFLEGLLASRP
                     EDGARLVADWFFREEDARTLDLIDRRLAEVEPSTREATLEKLLKSPRSGPKAFLWFGL
                     RAAQDDTFRARLTPTVLGRLLDAASWEDLGGGRAKLRELFDRTGVAAAWLVKQATLEE
                     AQQFLQALDRHNELEPHRRAALFAAAEMRFPDLRKGGDDSFFVTAEALEGKKRELERL
                     VEVDIPENTKGLAIARAEGDLSENFEYQARKQRQQDLGVRVARLQEELRKARVLDPST
                     VDTTEVRPGTRVTLRLLGGPREVTLLGPWDSKPEADVYSYLADASRGLLGRKVGDRVS
                     FLGDEGLIEAIEVWRSA"
     gene            7644..10025
                     /locus_tag="P5164_03915"
     CDS             7644..10025
                     /locus_tag="P5164_03915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HRY43074.1"
                     /translation="MRSRVLARARRIWKAIPRWALWGAAGLVLLFAVASAFVVAYYAR
                     VVSRTMDGRRWNLPTRLYSDAWVLRPGDALGPDDVERRLARLRYAPVAELPVSGGRYL
                     RTRSRIFVGVNPRETAYGRSPGLAARIDFSGRRISALRREPDEAPIPYVVFEPEVVGS
                     VFDRKMEDRTLVRLERVPKVVVDAILTTEDRDFFTHAGLDPTRLAGALLQGIARGRKV
                     RGTSTLTQQLVKNLFLTPERTLRRKAVEALLAVILDAKYGKEEILETYLNEIYLGQRG
                     AVSVTGVEEAARFYFGKPVSQLDLPESAMLAGLISSPGRYSPFRHPERAKARRALVLK
                     WMLEERKIDKAAHDAALTAPLTRVSKPMTGIEAPHFVDFVLTQVSESRSDLSGAGLSI
                     YTTLDPDMQEAAHAAVQEGLEELEKRFKRLRTAEGDQKLQAALIALDPQTGSIRALVG
                     GRDYQTSQFNRVVQAKRQPGSLFKPFVYLAAFERRDLSPPVTPVTILQDSPIALVFGK
                     TEEETWSPRNYDGEFLGPVTVRKALEQSRNIPTVRLAVWETGAGKTLLPDVIEAARKA
                     GITSRMKAYPSLALGSFECTPMEIAAAYSVFANGGILVKPNALLGLVGPEGRRIERKD
                     APLSRAADPAAVAVLDSILEGVVNRGTGASARGRGAQGIFAGKTGTTNDGRDAWFIGF
                     SPRLLVAVWVGFDDNRGLNLSGSTAAVPIYSGFVRRLPAHYFEEPFPQVPGTVSASVD
                     PTTGMLVTEECPTSVNELFVAGTEPTERCTVHASGGWPFGGGDTAGPSAGDPP"
     gene            10022..10894
                     /locus_tag="P5164_03920"
     CDS             10022..10894
                     /locus_tag="P5164_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HRY43075.1"
                     /translation="MTRARFDVPGVDLDLVARSHGWYDLAPFTWDEARRTLSFVFLAG
                     GAPERVVLSARNGGVRVVATASVRLARPVVARVLDLGSELGPFHALCAERRDEGFGWI
                     AERRAGRVLRAPTLFEDAVKVLCTTNCSWSLTRSMVTRMVGAFARGGAFPDAAFLASL
                     PERRLREELKLGYRAPFLRAFALRVASGALDLAAWEDPARSDTEVAELVRAEKGFGPY
                     AAETLLRLLGHHGHLGLDSWSRKKVAELRFGGRPVKDARVARFYAPFGRFAGLAFWLD
                     VTREWHDGRERLWP"
     gene            10970..11341
                     /locus_tag="P5164_03925"
     CDS             10970..11341
                     /locus_tag="P5164_03925"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRY43076.1"
                     /translation="MKKVPGPFRKFVETYPECGMTYEALAAASREAAGFPEKDAELLK
                     LALAMGSRLEGAVHSHARRALEAGAAASEVRGVALLALTTLGLPHAMMGTSWIEDVLR
                     EAEKAVEKAAKKAGKKTKRRK"
     gene            11420..12466
                     /locus_tag="P5164_03930"
     CDS             11420..12466
                     /locus_tag="P5164_03930"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018996755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HRY43077.1"
                     /translation="MSGIRERLEALGRLVGHTPLVAVDVTYRGNPRRVYAKAEQFNLT
                     GSIKDRMALHILRRARESGALSPGAPIGEATSGNTGISFSALGTALGHPVTIFMPDWM
                     SQERKDLIRSFGAEVRLVSAAEGGFLGSIRLSEEWAARTPGAFLPCQFANEANVEAHA
                     TTTGPEIRAQLAVLGLKPHAFVAGVGTGGTVMGTGRYLREQDPSVRVHPVEPANSPTL
                     HTGHKVGKHRIQGISDEFIPAIVKLDELDEIVSVDDGDAIVLAQRLARHGLGVGISSG
                     ANLLAALKVQESLGADAVVVTVFSDSNKKYLSTDLTREEPLKEGSMAREVEVTGMAAV
                     VRHCPSCGAGPGAS"
     gene            complement(12514..13740)
                     /locus_tag="P5164_03935"
     CDS             complement(12514..13740)
                     /locus_tag="P5164_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRY43078.1"
                     /translation="MSATVMEKPVTETPEVSKQKLIYLDNGATSFPKPEETYVAMDRF
                     YRAYGVNPGRSGFDLCLEAGSLVDRTRKLLCSFFGGTDPNRLVFGYNSTDALNLAIFG
                     LIAPGDHVVTTHLEHNATLRPLWMLEHEQGVKVDWVDFEADGWVDPQKVIDRITPGTK
                     AVVMNHGSNVIGTLQDLAPIGKVCRERGVHLIVDVSQSGGAVPVKLDEMGADVICFTG
                     HKSLMGPMGIGGMYVREGVEIRHTRAGGTGVKSAQRHHLDEYPYRMEYGTPNLPGIAG
                     LNAGVTWVLGQGIEAIHAREMALWKKLRDGLRAIDGVVLYCADDVPGKQRISVLSFNV
                     EGLEAADTGTMLDVDHDIACRTGLHCTPMVHEHLGTDRIHGAVRFGIGPFNTEAHVDA
                     AIAAVRDIASLRARKK"
     gene            complement(13725..15092)
                     /locus_tag="P5164_03940"
     CDS             complement(13725..15092)
                     /locus_tag="P5164_03940"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRY43079.1"
                     /translation="MTGPAPTSLESRIAALSDADLARGFEAFRSKLSAAEAAYLGACV
                     HCGLCAESCHYFKASPELENVPARKLDHVAAAFRRHATAAGRLLPGLVGARNFDRDLV
                     RAWVDAVFGRCSLCGRCSLNCTVGIALPRVFRAARAALASMGLVPDDLQATVDNSLVH
                     GNNMAIARTDWVETVQWLEEELQAETGDPQARIPLDKKGAKVLFTVNPREPKFFPLSL
                     QASAKVFWAAKEDWTLSSDPGWDLTNYGLFSCDDEKAGRITQNLVDAMERLGCETLVI
                     GECGHGYGAARWEGPEWLRGAFPFPVRSVLELMDEYLATGRIAVDPSCNPKPVTLHDP
                     CNLVRHGGVTEPQRRVLRRAARSFVEMSPSREESYCCSGGGGQLSMSAYKERRIASGE
                     PKARQIRETGASVVVAPCHNCIDQLMELNKEYKLGVQIRTVAEVLADAIVPGPREEGG
                     ACPRP"
     gene            complement(15089..>15659)
                     /locus_tag="P5164_03945"
     CDS             complement(15089..>15659)
                     /locus_tag="P5164_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43080.1"
                     /translation="ALAFRTRAWGRRALHAPPAGDAAAGARYAFTKGMLPQAKESVRT
                     HLPSYAAGLVFHGGVFASFGLLAASLAGFEPGSALLRLGGAAAGVGALAGLGLLGKRI
                     ASPVLRGLSAPDDYVANVLTSVFAALASLAAFAPDARAASLVAGAALLLYVPVGKIRH
                     CLLFFPARYEMGRFFGRRGTFPPASRRAA"
BASE COUNT         2156 a         5570 c         5776 g         2157 t
ORIGIN      
        1 agacggcgag gacgacgagc agggcgagcg gagtcttcac gaagccgaag agggcgtagc
       61 aggcggcgta gagcgtccag gcggcgagga ggaccctctt cctcccgtgc cggtccgaga
      121 ggccgccgcc ccatgtgccg agggcggccc ggatcgcgtt cgagaaggcc cagacgagag
      181 ggagtccggc cgcggagatg ccgacctcgc gcgccttcag gaggaggaac gtgtccgacg
      241 aggccgagag cgtgaacagg gcgactgcag cgacggcgcg gcgaaaggcg ggcgggagcg
      301 gggcggcggg cgtgcggacg accgcggcgg ctgccgcggg gcgcgaggtc tcccggacgc
      361 ccaggacgag gacgaggagg gcgagcgcgg ccgggacggc ggagagcgcg aggaccgtcc
      421 ggagagggag cgcgaggagg ccgaggagcg ccgcggcgag gagcggcccg gcgaccgcgc
      481 cggcgttgtc catggcacgg tggaggccgt aggcggcccc tcggcggtcg ggcggcgtca
      541 cctcggcgat gagggcgtcg cgcggggccg agcggactcc cttgccgatc cggtcgagga
      601 agcggacgag gaggacgtgc ggccagatcg aggcgagggc gacgaggggg cggacggccg
      661 aggagagcga gtacccggcg acgacgagcg gcttgcgccg cacggcgcgg tccgagagcc
      721 ggccggagac gaccttcacg gccgcggctg tcgcctcggc gatcccctcg atcacgccga
      781 gggccgcggc gggagcgccc gggagggagg cgaggaggag aggcaggagc ggaaagatca
      841 tctcggacga ggcgtcggtc aggaacgaca ccgcgccgag ggcgacgacc gtcctcggga
      901 ggcgggaggg agaatgcgtg ccggtgctgc tctccacgac ggccttcatt ctgcgtgtgg
      961 acgccctctc cgagaaggac gtggcggcga cgctcctgac ccgggatgcg ggggtcctcc
     1021 gcgccgcgtt ccggggggcg cgccgccgct tccgccgggg agcgctcctc gagctcctga
     1081 cggaggtctc cgtcacgctc ttccggaggg agggggccga gctcgtccgc ggggacgggc
     1141 tcgagctcgt caagagcgcg ttcggcctcg cttcgaggcc cgagaccgcg atgctcctgc
     1201 cgtacgtcgc ggagagcctc tcgacgttcc tcccggaggc cgaggcggcg cccgaggcgt
     1261 accggctcgc gcgccacgtc ctcgacgccc tcgaggaggg cgccccgccg acgctcgccg
     1321 cccgctattt cgagacctgg ctcctccgcc tcgccggtct cctgccgggc gggacggagt
     1381 gcgcctcctg cggtcggagc ctcgcgagcg gaggcgcccg cttcgacccc gagctgcccg
     1441 gcctcgtcgg cgacgagtgc gccccccccg gaacggtccc cgtctccggc gcggcgctgc
     1501 gcctcctcgc cgacgtccgc cggtcgcggc tggacgacct cgcgcggcga ggggccggag
     1561 atcccgagtc gctccgcgcg ctcgacgccc tgctgcgcga ggtgcgccgt cggttcctcg
     1621 gccacgagct gaagagccac cgcttcctcg acagcctctc gggcggcccg tgacgcgggc
     1681 gtccggccct gctaacctcc gctgctgccg caatttcgcg cgcctcagac cggagcgagc
     1741 atggacttcc agacgctgat cctgaagctg tccgaatact gggcctcccg cgggtgcatc
     1801 ctcgagcagc cctacgacac ggaggtgggc gcggggacca tgcacccggc gacgttcctc
     1861 cgggtcctcg gccccgagcc gtggcggtgc gcctacgtgc agccgtcccg ccggccggcc
     1921 gacgggcgct acggcgagaa cccgttccgc ctcgtgaagc actaccagtt ccaggtcgtg
     1981 atcaagccgt cgccggccga catccaggat ctctacgtcg acagcctccg ggccatcggc
     2041 atcgagccgg ccgagcacga cctgcgcttc gaggaggaca actgggagtc gccgacgctc
     2101 ggcgcctggg gcgtcggctg gcaggtcctc ctggacggga tggagatcac ccagttcacc
     2161 tactttcagc aggccggcgg catcgacctc gctccgatca ccgcggagat tacctacggg
     2221 ctcgagcggc tgacgatgtt cctgacgcgg acccggaacg tgatggacat cgaatgggcg
     2281 ccgggcgtgc cgtacggaga ggtgcggagg caggacgagt tcgaggtctc gaagtacggc
     2341 ttcgaggtcg ccgacaccga tctcctccgg cacctcttcg agaggctcga agcggagtcg
     2401 gggcgctgcc tcgcggccgg gctcgtcgtc cccgcctacg agaccgccct gaagtgctcg
     2461 cacgcgttca acctcctcga cgcgcgcggg gccgtctcgg cgaccgaacg cgtcggcgtc
     2521 atcaagcgcg tgagggacct cgcggtcggc tgcgcgaagg cgtgggtcgc ggcgcgcgag
     2581 agcctcgggc acccgctcct cccgaaggag acgccgaggc ccgaagcggc cgaagccggg
     2641 aacggggagg cctgagatgt cagaattcct gctcgagatc ctcgccgagg agatccccgc
     2701 gggcgtcctg tcgcaggcgc gcaacgacct cctccggggt ctcgccgacg ccctcgccgc
     2761 cgagcggatc gacgggacgt tcttcgccca ctccacgtcg cgccgcctcg tcctcttctc
     2821 tcgcgacctc cccgaggtcc aggaggacca cgaggtggag gccatcgggc cgtccgtcaa
     2881 ggtcgccttc gacgcggagg ggcgtccgac gcgcgccgcc gagggcttcg cccgcgggca
     2941 gggcgtcgcg gtagaggctc ttcgcgtcgt cgcgacgccg aagggggagt acgtctcggt
     3001 ccggaaggtc gtcgaggggc gattcacctc ggagatcctc ggcgaggtcg tcccgacggt
     3061 cgtcgcgaag atgtcgttcc cccggatgat gaggtggggc gacggacgcg acacctggat
     3121 ccggcccgtc cactccgtcg tctgccttct cgacgggctc gtcgtcccgg tgacgctctt
     3181 cggggtcgag tcgggacgga cgacgttcgg ccaccggacg ctcgcgccgg gccggatcgt
     3241 cgtcgtcggc gtcgacgact acctcgcgaa gctccgcgcc gctcacgtcg agccggacta
     3301 cggcgtaagg cgccggacgc tcctcgagaa ggccgaggcc ctcgcctccg aggccggctg
     3361 cacgccggtg gaggacagcg cgctcctcga cagcctcgcc cacctcgtgg agtggcccgg
     3421 cctcgtccgc ggctcgttcg aggccggata cctcgagctc cccgaggaga tcctcgtcac
     3481 ggcgatgcgc gagcaccaga aggtccttcc gacgcgcgac gcttcgggcg cgctcgcgcc
     3541 gcatttcctc gccgtcgccg accacgtcgg cgacccgatg gggcacatcg tccgcggaaa
     3601 cgagtgggtc ctgaacgccc ggttcgccga cgcccgcttc ttctgggacg aggacggccg
     3661 ggtccgcctc gaggaccgcg tcccccgcct cgcgcgcctt tccttccagg agaagctcgg
     3721 cgacacgctc cggaaggccg agcgggtcga ggctctcgcc tcgcgcctcg ccggcgacct
     3781 cggcctcggg gagtccgccg gcgaggccgc ccgggcggcc cgcctggccc gggccgacct
     3841 cgtcaccgac atggtgcgcg agttccccga cctccagggg gtcgtcggag ggctctacgc
     3901 ccggagggac ggcgagggtg aggcggtctg ccgggccgtc tacgaccact accgcccggc
     3961 cggcgtcgac gacgcccttc cggcggagcc cgtcggccgc gtcgtcgcgc tcgccgaccg
     4021 gctcgacacg ctcgccggct tcttcgcgct cggcctcgtc ccgacgggct cgcgcgaccc
     4081 gtacgccctc cggcgggcgg cgctcgccgt cgtccggata ctcctcgaag gagggcaccg
     4141 gctggagctc ccgtccgcga tcgccgccgc ccggcgcctg cacgaggtgt cgggcgcctc
     4201 ggaggaggag gtcgtctcga ctctccaccc cttcctcctc gagcgcctcc gcttcctgct
     4261 cgagcgccgc ggccaccggc acgacgagat cgaggccgtc ctcgcgactc cctgccccga
     4321 cgtgaccgac gcggccgagc gcgtcgccgc cgtctcgcgc gtgaggcgcc agcccgactt
     4381 cggcgctctc gccgcctcgt tcaagcgcgt gcagaacatc ctcgcccagg cgggcgtgac
     4441 gtcgggcggc gagatcgacc cgtcgctcat gagcgacgac gcggagaggc agctcgcgag
     4501 cgacttcttc caggcccgcg ggatcctcga cgagctgatc ggcgcgcgcc ggtacgacga
     4561 ggcgctctcg gtcatggcct ccctcgggcc ggtgctcgac cggttcttcg tcgacgtcat
     4621 ggtcctggtg gaggacgaag ccgtgcggcg caaccgcctc tcgctcctcg ccgcgatgcg
     4681 cgaccagttc ttccgcgtcg cgcggctgag cgagatccag gagtagggtc ccggcccccg
     4741 gctccgccgc ctcccttccg ggaagcggcg gagccggccg aggtcgtccg tgcggccgct
     4801 gcgcgcgatc gcagtatcct cccggcccga aggagaagaa gccatgagaa ccacccggcg
     4861 agccgtcgcc ctgtctgccg cattcgcgtt cgtgctctcc gccgctccgg tggaggccag
     4921 ggagctggcg ggggcctcga tgcccgacac cctctcggtg gccgggaaga cgctgaagct
     4981 gaacggaatg gggctccgca agaaggccgt gttcaaggtc tacgtcggcg ggctctacct
     5041 cgaggctccc tcctcggacg ccgccgcgat cctcgccgcc gacgcgccga aggcggtccg
     5101 gatgcacttc ctccggagcg tcgaccgcgg gaagctcgtg gagacgttcc gggagggctt
     5161 cgcggcgaac gccggcgcga aggcccaggc ccagaaggcg gggatcgaga agctcctcgc
     5221 gaccgtgacg gacgtgaagg acggatcggt gacgacgtac gtctacgcgc ccggcgccgg
     5281 gaccgtcgtc aaccgggacg gtaaggacgt cgcgacgatc gaggggaagg agttcgccga
     5341 ggtcctcttc tcgctctggc tcgggccgaa gccgccgtcg gaggacctcc ggagcggaat
     5401 gctcgggcgc tgaaggtccc gcggagtcga atcggagcgg ggcgggtaca ctcccgcccc
     5461 gtcatgaacc tcaacgagct ctcgtcgctc ctctccgaga agaaattcga cgtcgtcgag
     5521 aacgccgtca aggacgccct cctcgacgcc gaggagaacg cggagctcct cgcggcggcg
     5581 ttccgcggcc tcgcccgcgc gtccggccag aaggggcgcc tccagggcct ctccacgctc
     5641 gccgacgcga cgctgcgcgg cccgggcacg tctcccgcgg ccgcccgcct gcgctggggc
     5701 ctcctgaagg aggctgtccg ggccggcgcc acgccgtcct cgccggaggg cttccacaag
     5761 ctgttcgagg aggcgatccg tgcggcgtac cccgagtcgg cgtccctgac gacgttgctc
     5821 gggcgattcc ggttcctcga gtcgaaggag ccggcggacg gcctcgcgcg cgtcgagcgc
     5881 gccgagaagt ggctcccctt cgaggtcggg cgctgcttct tcatggcggt gcggggcgcc
     5941 gggaaggtcg tcgagacgaa cttcgccctc gacgccgtcc gggtcgactt cgagaagtcg
     6001 gccggagtct ccattccgat cggcgtcgcg gccaagagcc tcacgcccct ccccgagggg
     6061 cacttcctcc acgagaagct gaccgcccgc gaggcgctgg ccgagagcgt cctcgccgac
     6121 ccgtcggaag ggcttcggag gctcctcgac tcgttcggga cggcgctccc ggtctccgag
     6181 gtgaaggagg cgatggccgg cctcgtcccc gaggagcggt ggacctcgtg gtggaccgcg
     6241 gcgcgcaaga gtccgcacgt cgtcctccac ggctcgggca agaacgcctc cgtcgagtgg
     6301 acgcggacct cgcaggcggc cgacgccgcg atcctcgcga agttcgggaa ggcgcacacg
     6361 cccgagtccc gcctcgactt cttccggaag aacgcgaagc gcgacgcggc gctcgcggcg
     6421 aagatggggg cgaggctcgc cgaggacgcc cgggcgctgc gcgacgcgaa cccgccgctc
     6481 gcgtacgaga tcgcggtcgc gatcgagaag gtggaggggg ccgccctcgg attcgcgccc
     6541 gaggacctgg ttcccgaggc gccgctcccg atgctcggcc gtatcgggga ccgcctcgcg
     6601 cgggagcgct tcctcgaagg tctcctcgcc tcgaggccgg aggacggcgc ccgcctcgtg
     6661 gcggactggt tcttccgcga ggaggacgcc cggacgctcg acctgatcga ccggcggctc
     6721 gccgaggtcg agccctcgac gcgcgaggcg acgctcgaga agctcctcaa gtcgccccgg
     6781 agcgggccga aagcgttcct ctggttcggc ctgcgggccg cccaggacga cacgttccgg
     6841 gcccgcctca ccccgacggt gctcggacgg ctcctcgacg ccgcgtcgtg ggaggacctc
     6901 gggggcggac gcgcgaagct ccgcgagctc ttcgaccgga ccggggtcgc ggcggcctgg
     6961 ctcgtcaagc aggcgacgct cgaggaggcc cagcagttcc tccaggcgct cgaccgtcac
     7021 aacgagctcg agccgcaccg ccgcgccgcg ctcttcgcgg cggccgagat gcgattcccg
     7081 gatctccgga agggagggga cgacagcttc ttcgtcacgg cggaggcgct ggaggggaag
     7141 aagcgcgagc tcgagaggct cgtggaggtc gacatcccgg agaacacgaa ggggctcgcg
     7201 atcgcccggg ccgaagggga cctctcggag aacttcgagt accaggcccg caagcagcga
     7261 cagcaggacc tcggagtccg cgtggcgcgc ctccaggagg agctccgcaa ggcccgcgtc
     7321 ctcgacccgt cgacggtcga cacgacggag gtgaggcccg gcacgcgggt gacgctccgg
     7381 ctcctcggcg ggccccggga ggtgacgctc ctcggcccgt gggactcgaa gcccgaagcc
     7441 gacgtctact cctacctcgc cgacgcctcc cgtggcctcc tcggcaggaa ggtcggggac
     7501 cgcgtctcct tcctgggaga cgaagggctc atcgaggcga tcgaggtctg gaggagcgcc
     7561 tagcgcggcc ctcgccgccg ggctctgggc ccgccgtcga gatgccgagg gggaccggaa
     7621 agggcgttcg ctacactccc cgggtgaggt cccgagtcct cgcccgagcc cgccggatct
     7681 ggaaggcgat tccccgttgg gcgctctggg gcgcggccgg tctcgtcctc cttttcgccg
     7741 tcgcctcggc gttcgtcgtc gcctactacg cgcgcgtcgt ctcgcggacg atggacggga
     7801 ggcgctggaa ccttccgaca cggctctact ccgacgcgtg ggtcctccgc cccggcgacg
     7861 cgctcggccc ggacgacgtc gagaggcggc tcgctcggct ccggtacgcg ccggtggccg
     7921 agctccccgt ctccggcggc cggtacctcc gcacccgcag ccggatcttc gtcggcgtca
     7981 acccgcgtga gacggcctac gggcgctctc cgggtctcgc ggcacggatc gacttctccg
     8041 gccgcaggat ctcggccctc cggcgggagc cggacgaggc gccgatcccc tacgtcgtct
     8101 tcgagccgga ggtcgtcggc tccgtcttcg accggaagat ggaggaccgg acgctcgtcc
     8161 ggctcgagag agtcccgaag gtcgtcgtcg acgcgatcct cacgacggag gaccgggact
     8221 tcttcacgca cgcggggctc gacccgaccc ggctcgccgg cgccctcctg caggggatcg
     8281 cgcgcgggcg caaggtccgc gggacgagca ctctcaccca gcagctcgtc aagaacctgt
     8341 tcctcacgcc cgagcggacc ctgcggcgga aagccgtgga ggcgctcctg gccgtcatcc
     8401 tcgacgcgaa gtacggcaag gaggagatcc tcgagacgta cctcaacgag atctacctcg
     8461 ggcagcgggg cgcggtgtcg gtgacgggcg tcgaggaagc ggcccgcttc tacttcggca
     8521 agccggtctc gcagctcgac ctccccgagt ccgcgatgct cgccggcctc atctcctcgc
     8581 cggggcgtta ctcccccttc cggcaccccg agcgggcgaa ggcccggcgg gcgctcgtcc
     8641 tgaagtggat gctcgaggag cgaaagatcg acaaggccgc ccacgatgcc gccctcaccg
     8701 cgcccctgac ccgtgtctcg aagccgatga ccggcatcga ggcgccgcac ttcgtcgact
     8761 tcgtcctgac ccaggtgtcc gagtcccgga gcgacctttc gggagcgggc ctctcgatct
     8821 acacgacgct cgacccggac atgcaggagg cggcgcacgc ggccgtccag gagggcctcg
     8881 aggagctcga gaagcggttc aagcgcctgc ggacggccga gggggaccag aagctccagg
     8941 cggcgctcat cgcgctcgac cctcagaccg gctccatccg ggcgctcgtc ggcgggcgcg
     9001 actaccagac gagccagttc aaccgtgtcg tgcaggcaaa gcgacagccg ggctccctct
     9061 tcaagccgtt cgtctacctg gccgcgttcg agaggcgcga cctttcgccg cccgtcactc
     9121 cggtgacgat cctccaggac tccccgatcg ccctcgtctt cgggaagacg gaggaggaga
     9181 cctggtcccc gcgcaactac gacggggagt tcctcggccc cgtcacggtc cgcaaggcgc
     9241 tcgagcagtc tcgcaacatc ccgacggtcc ggctcgccgt ctgggagacg ggagcgggga
     9301 agacgctgct gccggacgtg atcgaggccg cgcgaaaggc ggggatcacg tcgcggatga
     9361 aggcttatcc gtcgctcgcg ctcgggtcgt tcgagtgcac gccgatggag atcgccgcgg
     9421 cctactccgt cttcgcgaac ggagggatcc tcgtgaagcc gaacgcgctt ctcggcctcg
     9481 tggggccgga aggacgccgc atcgaacgaa aggacgctcc tctctcccgg gccgccgatc
     9541 cggcggccgt cgccgtcctc gactcgatcc tggaaggcgt cgtgaaccgg ggcacggggg
     9601 cctccgcgcg cgggcgcggg gcgcagggga tcttcgccgg caagaccggg acgacgaacg
     9661 acggccgcga cgcctggttc atcggcttct cgccgcggct cctcgtcgcc gtctgggtcg
     9721 gcttcgacga caaccgcggg ctgaacctct ccggctcgac ggcggcggtc ccgatctact
     9781 ccggcttcgt ccgccgcctg cccgcccact acttcgagga gccgttcccg caggtcccgg
     9841 ggaccgtgag cgcctccgtc gacccgacga ccgggatgct cgtgaccgag gagtgcccca
     9901 cgtccgtgaa cgagctcttc gtcgccggaa cggagccgac cgagcgctgc acggtgcacg
     9961 cgtccggcgg ctggccgttc ggcggcggag acacggctgg gccctcggcc ggcgatccgc
    10021 cgtgacccgg gcgcggttcg acgtcccggg cgtcgacctc gacctcgtcg cgcggagcca
    10081 cggctggtac gacctcgcgc cgttcacgtg ggacgaggcc cgtcggaccc tttccttcgt
    10141 cttccttgcc ggcggggccc ccgagcgggt cgtcctctcg gcgcggaacg gcggcgtccg
    10201 ggtcgtcgcg accgcgtcgg tccgcctcgc ccgccccgtc gtcgcccggg tcctcgacct
    10261 cggctcggag ctcgggccgt tccacgcgct ctgcgccgag cggcgggacg aggggttcgg
    10321 atggatcgcc gagcggcgcg cgggccgcgt cctgcgagcg ccgacgctct tcgaggacgc
    10381 cgtgaaggtc ctctgcacga cgaactgctc ctggtccctg acgcgctcga tggtgacgcg
    10441 gatggtcggc gctttcgccc ggggcggggc gttccccgac gcggcgttcc tcgcgtccct
    10501 accggagcgg cggctccgcg aggagctgaa gctcgggtac cgagcgccgt ttctccgcgc
    10561 gttcgccctg cgcgtcgcct ccggcgccct cgacctggcg gcgtgggagg accctgcccg
    10621 ctccgatacg gaggtggccg agctcgtccg cgcggagaag gggttcggcc cgtacgcggc
    10681 cgagacgctc ctgaggctcc tcgggcacca cggccacctc ggcctcgact cctggtcccg
    10741 gaagaaggtc gcggagctga ggttcggggg ccggccggtg aaggacgcgc gcgtcgcccg
    10801 cttctacgcc ccgttcgggc gcttcgcggg cctggcgttc tggctcgacg tgacgcggga
    10861 gtggcacgac ggtcgggagc ggctctggcc gtaagcccgg actcgacgcc ggcgttcacc
    10921 cgcggcgcgg ctccgggccg cgctcctaca atgcagggtg gaggatccga tgaagaaggt
    10981 gcccggtccg ttccggaagt tcgtcgagac ctacccggag tgcgggatga cctacgaggc
    11041 tctggcggcc gcgagccgcg aggcggccgg cttcccggag aaggacgccg agctcctcaa
    11101 gctcgcgctc gcgatgggct cgcgcctcga gggggcggtc cactcgcacg cccgccgcgc
    11161 gctcgaggcg ggcgccgcgg cgtccgaggt gcgcggcgtc gcgctcctcg cgctgacgac
    11221 gctcggcctc ccgcacgcga tgatggggac ctcctggatc gaggacgtcc tgcgcgaggc
    11281 ggagaaggcc gtggagaaag cggcgaagaa ggccgggaag aagacgaaga ggcggaagta
    11341 gggccggcgg cgcgacggtc ggaccgcacg gcggcggaac gcgtccgcgt cgagcgcggc
    11401 gcgcgggggg gacggtggga tgtcgggaat tagggagagg ctcgaggcgc tcggccgcct
    11461 cgtcggacac acgccgctcg tcgcggtcga cgtcacttac cgcgggaatc cgcgccgcgt
    11521 ctacgcgaag gccgagcagt tcaacctgac cgggagcatc aaggaccgga tggcgctcca
    11581 catcctccgg cgcgcgcgcg agagcggggc gctctcgccc ggggcgccga tcggggaggc
    11641 gacgagcggg aacaccggca tctccttctc ggcgctcggg accgccctcg gccacccggt
    11701 gacgatcttc atgccggact ggatgagcca ggagcgcaag gacctgatcc gctccttcgg
    11761 cgccgaggtc cgcctcgtga gcgccgcgga gggcgggttc ctcggctcca tccgcctgag
    11821 cgaggagtgg gcggcccgca cgcccggagc cttcctcccg tgccagttcg cgaacgaggc
    11881 gaacgtcgag gcgcacgcga cgacgacggg gccggagatc cgggcccagc tcgcggtgct
    11941 ggggctcaag ccccacgcgt tcgtcgcggg ggtcggcacc ggcgggacgg tgatggggac
    12001 ggggcgctac ctcagggagc aggacccgtc ggtgcgggtc cacccggtgg agcccgcgaa
    12061 ctcgccgacg ctccacacgg gccacaaggt cggcaagcac cggatccagg ggatctccga
    12121 cgagttcatc ccggcgatcg tgaagctcga cgagctcgac gagatcgtca gcgtggacga
    12181 cggcgacgcg atcgtcctgg cgcagcggct cgcgcgccac ggcctcggtg tcgggatcag
    12241 cagcggcgcg aacctcctcg cggcgctgaa ggtccaggaa tcgctcggag cggacgccgt
    12301 cgtcgtcacc gtcttcagcg acagcaacaa gaagtacctc tcgacggacc tcacgcggga
    12361 agagcccctc aaggagggct cgatggcgcg cgaggtcgag gtgacgggga tggcggcggt
    12421 cgtccgccac tgcccctcct gcggggccgg cccgggcgcg agctgacggc acggcccggc
    12481 ggcgcgccgc cgggccgcgg ccgctccgga ctcctacttc ttccgggccc tcagcgacgc
    12541 gatgtcccgg acggcggcga tcgccgcgtc gacgtgcgcc tccgtgttga acgggccgat
    12601 cccgaagcgg acggcgccgt ggatcctgtc cgtcccgagg tgctcgtgga ccatcggggt
    12661 gcagtggagg cccgtgcggc aggcgatgtc gtggtcgacg tcgagcatcg tcccggtgtc
    12721 ggccgcctcg agaccctcga cgttgaagga gaggacgctg atccgctgct tgccggggac
    12781 gtcgtcggcg cagtagagga cgacgccgtc gatcgcgcgg aggccgtcgc ggagcttctt
    12841 ccagagcgcc atctcacgcg cgtggatcgc ctcgatcccc tggccgagga cccaggtgac
    12901 tccggcgttc aggcccgcga tcccgggcag gttcggcgtg ccgtactcca tccggtaggg
    12961 gtactcgtcg aggtggtggc gctgcgcgct cttgacgccg gtgccgcctg cgcgggtgtg
    13021 gcggatctcg accccctcgc ggacgtacat gccgccgatc cccatcggcc ccatcaggct
    13081 cttgtggccg gtgaagcaga tgacgtcggc gcccatctcg tcgagcttga cggggacggc
    13141 gccgccggac tgcgagacgt cgacgatgag gtggacgccc cgctcgcggc agaccttccc
    13201 gatcggggcg aggtcctgga gcgtgccgat cacgttcgag ccgtggttca tcacgacggc
    13261 cttcgtgccg ggggtgatcc ggtcgatcac cttctgcggg tcgacccagc cgtcggcctc
    13321 gaagtcgacc cagtcgacct tcacgccctg ctcgtgctcg agcatccaga gcgggcggag
    13381 cgtggcgttg tgctcgaggt gcgtcgtgac gacgtggtcg ccgggcgcga tcaggccgaa
    13441 gatcgcgagg ttcagcgcgt cggtggagtt gtagccgaag acgagccggt tcgggtcggt
    13501 cccgccgaag aaggagcaga ggagcttgcg ggtccggtcg acgagggagc cggcctcgag
    13561 gcagaggtcg aagccgctcc gccccgggtt gacgccgtag gcgcggtaga accggtccat
    13621 cgcgacgtag gtctcctccg gcttcgggaa ggaagtcgcg ccgttgtcga ggtagatcag
    13681 cttctgcttc gagacctcgg gcgtttcggt cacgggcttc tccatcacgg tcgcggacat
    13741 gctccacctt cctctcgcgg accagggacg atcgcgtcgg cgaggacctc cgcgacggtc
    13801 cggatctgga ctcccagctt gtattccttg ttcagctcca tgagctggtc gatgcagttg
    13861 tggcacggcg cgacgacgac ggaggctccg gtctcgcgga tctgccgcgc cttcggctcg
    13921 ccggaggcga tccgcctctc cttgtaggcc gacatcgaga gctggccgcc cccgccgctg
    13981 cagcagtagc tctcctcgcg gctggggctc atctcgacga aggagcgcgc ggcgcgccgc
    14041 aggacccggc gctgcggctc cgtcacgccc ccgtggcgga cgaggttgca cgggtcgtgg
    14101 agcgtgacgg gtttcgggtt gcacgacggg tcgacggcga tgcggccggt cgcgaggtac
    14161 tcgtccatca gctcgaggac gctccggacg ggaaacggga aggctccccg gagccactcg
    14221 ggcccctccc accgggcggc cccgtagccg tgcccgcact cgccgatcac gagggtctcg
    14281 cacccgaggc gttccatcgc gtcgacgagg ttctgcgtga tccggccggc cttctcgtcg
    14341 tcgcagctga agaggccgta gttcgtcagg tcccagccgg ggtcgctcga gagcgtccag
    14401 tcctccttcg cggcccagaa gaccttcgcg ctcgcctgca ggctcagcgg gaagaacttc
    14461 ggctcgcggg ggttcaccgt gaagaggacc ttcgcgccct tcttgtcgag ggggatccgg
    14521 gcctgggggt cgcccgtctc ggcctgaagc tcttcctcga gccactgcac cgtctcgacc
    14581 cagtccgtcc tcgcgatcgc catgttgttg ccgtggacga ggctgttgtc gaccgtcgcc
    14641 tggaggtcgt cggggacgag gcccatcgac gcgagggcgg cgcgcgcggc gcggaagacc
    14701 cgcggcagcg cgatgccgac cgtgcagttc agcgagcagc gcccgcagag cgagcagcgg
    14761 ccgaagacgg cgtcgaccca ggcccggacc agatcccgat cgaaattccg ggctccgacg
    14821 agtccgggga ggaggcggcc ggccgcggtg gcgtgccggc ggaacgccgc ggcgacatgg
    14881 tcgagcttgc gggccgggac gttctcgagc tcgggcgagg ctttgaagta gtggcagctc
    14941 tccgcgcaga ggccgcagtg gacgcacgcg ccgaggtacg ccgcctcggc cgcgctcagc
    15001 ttcgagcgga acgcctcgaa gccgcgggcg aggtcggcgt cggagagggc cgcgatccgg
    15061 ctctcgaggg aggtgggagc aggcccggtc atgccgccct ccgcgaggcc ggcgggaacg
    15121 tcccgcgccg gccgaagaag cggcccatct cgtatcgggc cgggaagaag aggaggcagt
    15181 gccggatctt gccgaccggc acgtagagga ggagcgcggc gccggcgacg agcgaggccg
    15241 cgcgcgcgtc cggagcgaac gcggcgaggg aggcgagggc tgcgaagacc gacgtcagga
    15301 cgttcgcgac gtagtcgtcg ggggccgaga gcccgcgcag gaccgggctc gcgatccgct
    15361 ttccgaggag gccgaggccg gcgagggccc cgacccccgc cgcggcgccg ccgaggcgga
    15421 ggagggcgga gccgggctcg aatccggcga ggctcgccgc gaggaggccg aaagaggcga
    15481 agaccccgcc gtggaagacg aggccggccg cgtacgacgg gaggtgcgtc ctcacgctct
    15541 ccttcgcctg cgggagcatc cctttcgtga acgcgtagcg ggcgccggcg gcggcgtcgc
    15601 cggccggcgg cgcgtggagc gcgcgccggc cccacgcgcg ggtccggaac gcgagggcc
//
LOCUS       DAOTEZ010000047        15530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2594_length_15530_cov_6.574346, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000047 DAOTEZ010000000
VERSION     DAOTEZ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15530
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2594_length_15530_cov_6.574346"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="P5164_03950"
     CDS             <1..239
                     /locus_tag="P5164_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY43081.1"
                     /translation="EPHLGLVSAAEPEALLESAASRAWPLRRLTDAYVRLVLAEAGGN
                     VAEAARRLGIARKTLYEHLGSGLPSTLCGSGRRV"
     gene            393..875
                     /locus_tag="P5164_03955"
     CDS             393..875
                     /locus_tag="P5164_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HRY43082.1"
                     /translation="MATKSASATKKAPAKSATKASVKVPSKAPARPAAKAAPKAVERA
                     PKDPLEKHRKHLLAKQEELLHDLSRNRQVTDESVDEQAQDMVDRATSAYTREFAFSLS
                     ESDRRTLLLIDQALLRLDQGTYGTCVHCQGHVQEKRLEAVPWARHCLECQELQDKGLL
                     "
     gene            893..2326
                     /locus_tag="P5164_03960"
     CDS             893..2326
                     /locus_tag="P5164_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43083.1"
                     /translation="MRGDPFGPLAPLLAAVAAAPLPPVLVVLGDDEWFVGEAARRLAK
                     AFGTAFPDGEVSEYDGTGNGVREAVADAATIALFATNRLVSLDVTDLLRTKKLSADEI
                     DTLLDDAAEAGLGAPPGAPSEPRVLERLARKARALARETGAWVEGDAAESARKLAGRG
                     KRSDRAPELARLLDWPVEDGEAAETALGRLLEYVTRAGAGDNVLLLTAVSPDPEHAVL
                     SELARAPRAALLARDDAARRERLHALGLERVLERGKTAEPDVFETLTERGRLSARAFL
                     SELDRLVDTAVGNRVTSEAAARLVVDERKEYGSDFVEAVARRRFLEALRIAERLLAAD
                     DFTAFRPFGKDGPAAAAKKGPKGEAAFFPLLGLLGAELRRMLALKAALAEPGADPGPR
                     RADYRTFVDRLLPRLKASRAGAAPIPLDGHPFVLHKAYLASFDWSLDELADALVGLAD
                     VDRGVKTGLGSGAELLQTWLLLRNPAL"
     gene            2482..2781
                     /locus_tag="P5164_03965"
     CDS             2482..2781
                     /locus_tag="P5164_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43084.1"
                     /translation="MNRRQVFGAAAAIMAAGMLSATYASADDKKPAGETPKAEKKTIK
                     CMGGNACKGKSECGVPGAHTCHTRNACKGKGWVMVASEKECEELKAKNAAPAKKS"
     gene            2778..3587
                     /locus_tag="P5164_03970"
                     /pseudo
     CDS             2778..3587
                     /locus_tag="P5164_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005903909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
     gene            3804..4604
                     /locus_tag="P5164_03975"
     CDS             3804..4604
                     /locus_tag="P5164_03975"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HRY43085.1"
                     /translation="MLGLVRAPEGVAKALEERGGAREEVAALFAGDERLPAVERLELY
                     AGMYFFRLRDSLAEDFARTAAELGEARWHNLVTDYLLAHPPTRWSLRWAGEALPVFLR
                     SHPYGAERPWLADAATLEQARNEAFQARDATPLRPEELALVSPEEWPELRFAPAPGTA
                     VVASRWDLAGWWDDAASAEPPDALEEEATLLVFRDAEDDVRHEAIAPEEAEAARLLLA
                     GAPFAEVCGAFADAATDETDAARRALALVRRMGSCLHSTLRGNAECRV"
     gene            4620..8012
                     /locus_tag="P5164_03980"
     CDS             4620..8012
                     /locus_tag="P5164_03980"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRY43086.1"
                     /translation="MERFSRRDGRFVALCVAVIVAGAAVGVPLFPRAFPEASIDFRVT
                     REEAQQAAERALARRGFDLAGRRALAIFDHDDTAKVFLERELGLERARSLLGGDVPVW
                     RWSFRFVKPLEKGELLAFVAPSGELLSFRRVLPEGAPGGDPGSARARTLAEETLREHR
                     GLDAATLKLVEAREEKKPARVDRTFVWESPTASWKGAAMRYLVEVQGAQVGRSTLWLE
                     VPEAWRQEYATLRSKNHAAGAAATFGLVLTAVALVAAFFGRLRRRDVPWRTAIGFGLA
                     GAALQAAASLNELPLTLFGYDTTESWGAFVGQAVLADLGSASLLGVALLLLVAGGEPE
                     YRQAYPDKPALGRLFTRRAFGTKRFFLGLLAGYALTAGFFAWQVLFYLSAERLGAWSP
                     ADVPYSNLLGTMFPWLGVLLMGFVPATTEEFSSRMFSIPFLQRFAPTWVAVGLPALIW
                     GFAHAGYPNQPFWVRGVEVGLAGVVVGVVMLRRDLFPLLVWHFTVDAVYTSLLLVRSS
                     SPYFVVSGLLSAGALVLPLVVAGALAWRRGGFVPESGLTNGDVGSAPPVSPGAADEAP
                     AFAEPRPLPSRAVVAVLAGASALVLAGAAAFLPSGKLDAAARVTRTEALDAARAFVRG
                     QGDDPARYLVTARIDSALPALEEASETGRGLLPYRWERSAERWLLEKGGPETLRRWAG
                     GVLPGGVWQVRLARERDRHAWWVVVDARDGRVVSFARDLPEEEAGAALAPDAALGRAK
                     EAARARGVDPDGLAVVSAVEKERPARRDHRIVFESTDDAAGEARRRVLVDVAGGRTSL
                     VATALKLPEEWVRACRRSTGATWAALGAKVVGFGTVVGLGLVVLLLAHRGGRVRWREA
                     SRWAALLTLPALLERASRLPTLLLAWPPETPLAAWSVSAALGILAGLLVSYALALVAC
                     GLVSAAAPHALGLARRRSPGARRRALVAGGATALLVLSARRLADGLRGLFPASAGVGG
                     LDLPAGVDGWLPAASSLASATELALLAAGGAAVAALLARDFRRSRGGWVLLGLAVTGC
                     WAPLEAWDAAEVVVPLLVGALPAAALALGAALLLKDDPRAFATAAAGIVLLPEGAAFA
                     ASGVPSWAWSGGLCLAAAILVALAPGRKDAGAIPPRPSPRAG"
     gene            complement(7978..8850)
                     /locus_tag="P5164_03985"
     CDS             complement(7978..8850)
                     /locus_tag="P5164_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43087.1"
                     /translation="MSAKKRLVDLPAAPPAPEGWGGRWLAAASGAGKARLEKGRSLVA
                     EGALTALSVKPGLVEAEVRDRGRSVLHVRLRLRTLNEKVFDGALKAFAERARTAAELL
                     SGLLPEESDDVFAAAGGALLPVSPTEVAVTCACPDGAACRHAAAVHVALAERFDRDPF
                     LLLLLRGKGRDEVLARLREARTRRAAPAAPPAPAARAAAASAAPLEPLPDVRLDAFYR
                     PLVPLATLKTRFLSPEHPDALLTRLGPPPLEDEEAARLLVEMHRAVGRGAADRLSESE
                     WRRVSPRGAKASGE"
     gene            complement(8852..11947)
                     /locus_tag="P5164_03990"
     CDS             complement(8852..11947)
                     /locus_tag="P5164_03990"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRY43088.1"
                     /translation="MLVLHGNPLPPTGPLPGTGFFLWGEGPFVGARQRPEHPRHVDPS
                     LLAAALRSAGLAVATGGARPLRLVLTVPTLRSRPVPTRSSLREDGDYAAGETVLKPLE
                     VEGLLVPSERAFEALLSLAEEPDRAGLSLGDDTEYWSAVAGWVLDLLHRRRFVPAVED
                     GHARWRPVLTDPHEKERVEVFAQAMPAASRAIAWPGSASEGLYPAAAFLLRGVVDDLT
                     DAAARALLAETLPEPRRRGLPGPEGLALARLAVRRDGREDETAAEAVPADVAERFGAW
                     SIPLLEALPEGELRLGVRLLPPEPEAEGAPSWRLGYHLEAADDPSLKLYAEEIWNETA
                     SSLRRLGRRFVNPQETLLARLGGVAKLCEPIRRSLEERHPVGAPLALEEAWRFLSAEA
                     PLLSEAGVQLLLPAEGRTTKPSLRLNAAESRWKSGVAVTRFGLGTLVDFDWKVAVGDL
                     LLSPEEFEQLASRKVPLVEVRGEWVLLDPQSVQRTLALFEKKPSGRASLADFLRMASG
                     LDEDDDAAPGVDVLTADGWLADFLQADAEKDAPFVTPKELEGRLRPYQEKGVTWLRFL
                     LSRGLGSCLADDMGLGKTIQLLATLLTAREAGEAIRPSLLVCPTSVVENWVQEAHRFA
                     PTLTVAVHHGPERAAGDDFFALVKKTDLLVTTYSLAHRDRALLTDVTWEYLALDEAQN
                     IKNPGTAQSRAVRALRSTRRAALTGTPLENRLSELKAILDFLNPGLLGSDESFRRTFS
                     IPIERHHDPVARERLRRLTSPFLLRRTKTDPAIAPDLPDKIETKEWVGLSREQATLYR
                     ATTKALLEGIGKAQGQSRRAKVLLLLLRLKQICNHPALFLGDGSRLDGRSSKLTRLLA
                     MLEETEAENRPALLFTQFSEMGHLLVRALRDKFDKEILFLHGGVPRLARAEMVRRFQE
                     DDDPPLFFVLSLKAGGAGLNLTRASHVFHVDRWWNPAVEDQATDRAFRIGQTRHVQVH
                     KFVCRGTLEERIDHMIDEKKELARSIIGAGESWITELSNEELAELVALSRDAVDAGEG
                     S"
     gene            12011..12523
                     /locus_tag="P5164_03995"
     CDS             12011..12523
                     /locus_tag="P5164_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43089.1"
                     /translation="MKPYEIFRRLSTAEADALVLSACEDDEIPDKVAGSVLTLQRVPL
                     KRFERMTDEVRRALVRKTLRDRRAEDLALFVLSAGLVRERGEMIEGFLSSLDLPHDGP
                     NLTADGPVAEPPRERLVKAVADLAGKHGARDAALFLHAFVEQPDVAWPVLAGLLDGDP
                     KLALEDRSAD"
     gene            12616..13107
                     /locus_tag="P5164_04000"
     CDS             12616..13107
                     /locus_tag="P5164_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HRY43090.1"
                     /translation="MIPEKLKAALDEHVSAEVGAAYLYLAMSADFESKSFKGFARWMR
                     IQFQEEMAHALKFVDYMLSRGATIGWKDAKAPASTFGSPLETFEKTLAHEKDVTARIH
                     KLYHLAAAEGDTATQVFLQWFVTEQVEEEARVTEIVDKIRMVSDRPGSVLYLDKEYGK
                     RQA"
     gene            complement(13348..14145)
                     /locus_tag="P5164_04005"
     CDS             complement(13348..14145)
                     /locus_tag="P5164_04005"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HRY43091.1"
                     /translation="MRRVRFHARLLALYFAQYAKARLAYRADFAAAALASFLGTAASF
                     SVVLLLFSRFPHLAGWTFPEIVFLYGFSLLPYGLFSVVSSNLWEFADRYLVEGRFDRI
                     LLRPVSPLFQVLFESFRLESLQEVATGAVAIGWAVSRLPDVSPLGLALLPLWAVFGAV
                     LYVSVFGLLTAASFWVEDRVGLAPPVFNLMSFGRYPITIYDLKVKLLLSTVIPFAFAS
                     FYPTALALRRGEYLPYFWAVPLVAVVFALLNVALWRRGIARYGSTGS"
     gene            complement(14148..14969)
                     /locus_tag="P5164_04010"
     CDS             complement(14148..14969)
                     /locus_tag="P5164_04010"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HRY43092.1"
                     /translation="MTALAAAARPYLAFARTSFLNILAYRARYFVGVLTYFFNATVWY
                     YIWRALFHEKGEGATLGGFTFPQMLTYVATGWALRSFYFNEVDRDIASQVREGRLAMT
                     LVKPVDVQAMTLSQALGESAFRLVLFTLPIGLLLVVVYPVLPPASPAALALYLLSGLL
                     SALLVGAINFCVGLVAIRTTSILGVLRAKYLVLEVLSGLLVPVAFFPEPVRKVLVWLP
                     FLHMNDTPGRIWLGLATGADAARALGLQAAWVVALLLLGRLLWKATARRIEVQGG"
     gene            complement(14974..>15530)
                     /locus_tag="P5164_04015"
     CDS             complement(14974..>15530)
                     /locus_tag="P5164_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HRY43093.1"
                     /translation="EPPLLFLDEPTIGLDVVAKANVRSFLKEANKLLETTVILTTHDL
                     DDIEELCERVVLIDHGQVLWDGSLRGLRDEHLPEARIRFDLPQPPPGDPQDLVIGPWT
                     AGRLSPGRYLLAVDKREASSADVIREIVRGLPVLDLAVSEPDIEEVVSRIYRENAALR
                     KPDAPPPSEGAAPGFVSHRGVRRG"
BASE COUNT         2081 a         5400 c         6013 g         2036 t
ORIGIN      
        1 acgagcccca cctcggcctc gtctccgccg ccgagccgga ggccctcctc gagtccgccg
       61 cctcccgagc ctggccgctc cgccgcctca ccgacgccta cgttcgcctc gtcctcgccg
      121 aggccggcgg aaacgtcgcc gaggccgccc gccgcctcgg catcgcccgc aagaccctct
      181 acgagcacct ggggtcaggt ctaccttcta cactctgcgg ctcgggccgc agagtgtaga
      241 aggtagacct gacccccttc ccgattcgtc tgccgcgggc ggcgtggggc tgtgctagag
      301 tcccgcgatt ccggagaggc cggcacggtg gattcggccc gccggacggg cgccacgagg
      361 cgtccgggaa cgacggaagg gggagtgtcc ccatggcgac gaagagcgcg tccgcaacga
      421 agaaagcccc cgcgaagagc gcgacgaagg cttccgtgaa ggtcccgtcg aaggcccctg
      481 cccggccggc ggcgaaggcc gccccgaagg ccgtcgaaag agctccgaag gaccccctcg
      541 agaagcatcg caagcacctc ctcgccaagc aggaggagct ccttcacgac ctctcccgca
      601 accggcaggt gaccgacgag tcggtcgacg agcaggcgca ggacatggtc gatcgggcca
      661 cctcggccta cacgcgggag ttcgccttct ccctttccga gtccgaccgt cgcacccttc
      721 tcctgatcga ccaggcgctc ctccgcctcg accagggcac ctacgggacc tgcgtccact
      781 gccaggggca cgtccaggag aagcgcctcg aggcggtccc gtgggcacgg cactgcctcg
      841 agtgccagga gctgcaggac aagggcctgc tctgagcccg gcgaggctcc ccgtgcgcgg
      901 cgaccccttc ggccccctgg cgccgctgct cgcggcggtc gccgctgctc cgctcccacc
      961 ggtcctcgtc gttctcggcg acgacgaatg gttcgtcggc gaagccgcgc gccgcctcgc
     1021 gaaagccttc ggcaccgcgt ttcccgacgg cgaggtctcg gagtacgacg gcaccggcaa
     1081 cggggtccgg gaggcggtcg ccgacgcggc caccatcgcc ctcttcgcca cgaaccgcct
     1141 cgtctcgctc gacgtcaccg atcttctccg gacgaagaag ctctcggccg atgagatcga
     1201 cacactcctc gacgacgccg ccgaggccgg cctcggcgcg ccgcccggcg caccgtcgga
     1261 gccgcgcgtc ctcgagcgcc tcgcgcgcaa ggcccgcgcc ctcgcgcgcg agaccggcgc
     1321 ctgggtcgag ggggacgccg ccgagagcgc ccgaaagctc gcgggacgcg gcaagcgctc
     1381 cgaccgcgct cccgagctcg cgcgcctcct cgactggccc gtcgaagacg gcgaggcggc
     1441 cgagaccgcg ctcggccgcc tcctcgagta cgtcacccgc gccggcgccg gcgacaacgt
     1501 cctcctcctc accgccgtct cgcccgaccc cgagcacgcc gtcctctccg agctcgcccg
     1561 cgccccgcgc gccgccctgc tcgcccgcga cgacgccgcg cgccgggagc gcctccacgc
     1621 gctcggcctc gagcgcgtcc tcgagcgggg gaagacggcc gagcccgacg tcttcgagac
     1681 gctcaccgag cggggccgcc tctcggcccg cgcgttcctc tccgagctcg accgcctcgt
     1741 cgacaccgcc gtcggcaacc gcgtcacctc cgaggccgcc gcgcgcctcg tcgtcgacga
     1801 gcggaaggag tacggctccg acttcgtcga ggcggtcgcc cggcgccgct tcctcgaggc
     1861 cctccgcatc gccgagcgcc tcctcgccgc cgacgacttc accgcctttc gtcccttcgg
     1921 caaggacggt cccgcagccg ccgcgaagaa gggaccgaag ggcgaggccg ccttcttccc
     1981 gctcctcggc ctcctcggcg cggagctccg tcgcatgctc gccctgaagg ccgcgctcgc
     2041 cgagccgggc gcggaccccg gcccgcggcg cgccgactac cggacgttcg tcgatcggct
     2101 ccttccgcgc ctgaaggcct cccgcgcggg ggcggcgccg attcccctcg acggccaccc
     2161 cttcgtcctc cacaaggcgt acctcgcctc cttcgactgg tccctcgacg agctcgccga
     2221 cgccctcgtc ggcctcgccg acgtcgaccg cggcgtcaag accggcctcg gctccggggc
     2281 cgagctcctc cagacctggc tcctcctgcg aaaccccgcg ctctgacgcg acggcccgac
     2341 cgccaggcgc gactcgccga ggggcccggc ccggctccgg gctcgagcgc ccttgcgccg
     2401 agccccgctt ccacctagga tctgcaactt tgtctcagga aatgaccggt cgcggagagc
     2461 gcgggcggca ggaggttcgg gatgaatcgc aggcaagtgt tcggggcggc cgcggcgatc
     2521 atggcggcag ggatgctctc ggccacgtac gcgtccgccg acgacaagaa gccggccggc
     2581 gagacgccga aggccgagaa gaagacgatc aagtgcatgg gcgggaacgc ctgcaagggg
     2641 aagagcgagt gcggcgtgcc gggagcgcac acgtgccaca ctcggaacgc gtgcaagggg
     2701 aagggctggg tgatggtcgc ctccgagaag gagtgcgagg agctgaaggc gaagaacgcc
     2761 gccccggcga agaagtcctg accgcggcgc cgttcctcgg tcacggcatc ggcctgcgcc
     2821 gcgagcatta cgcggcgatc ctcgcgggag aggccgcctg cgactggctc gaggcgatct
     2881 cggagaacta catggccgtc ggcggacgcg cgaggaaggt cctcctcgcc gtccgcgagc
     2941 ggtacccgat gacgctccac ggcgtctcgc tcggcatcgg gaacacggat ccgctcgacg
     3001 aggcctatct cgacgccctc ggagcgctcg cggcggaggt ggagccggcc tggatctccg
     3061 accacctctg ctggaccggc gtcgagggac gtacgtcgca cgacctcctg ccgctgccgt
     3121 acacggagga ggctctctcg aacgtgatcg agcgcgtgcg gcgcgttcag gatcgcctcg
     3181 ggcgtccgct cgtcctcgag aacgtctcga gctacgtgac gtggcgggcc tcgacgatcc
     3241 cggaggagga gttcctcgcg gagctggcgc gccggtcggg ctgcggtctc ctgctcgacg
     3301 tgaacaacgt cttcgtgacc gcgacgaacc acgggcgcga tccgcggcgc tggctggagg
     3361 cgatcccgca gggaagcgtc tggcagctcc acctggccgg accctcggag gcgggaccgc
     3421 tgctcgtcga cacccacgac cacccggtgc gccccgaggt gtgggagctc tatcgcgagg
     3481 cggtgcgccg cttcggcgag gtctcgacgc tcgtggagtg ggacgaccgc attcccgtga
     3541 tcggcgtcgt cctcgcggag cgcgacaagg cggccgcgat cgcggccgag gtccgggagg
     3601 ggcgaagcgc ccgggtcgaa ggggacggtc cgcccgaagc cgaagccgcg cgcccggcgc
     3661 gtgccggggc atcggcgcaa gagccgctcc gaagagcggc gcgggtggac acgacggaac
     3721 acggacctgc cccggttctc ggtgcgccgc caacggaaga cgggcccggc cccgccttcg
     3781 ggctgcgggg cgcgcagggg gtgatgctgg gcctggtgcg ggcgccggag ggggtggcga
     3841 aggcgctcga ggagcgaggc ggggcgcggg aggaggtcgc ggctctcttc gcgggcgacg
     3901 aacggctgcc ggccgtcgag cggctcgagc tctacgccgg gatgtacttc ttccggctgc
     3961 gtgactcgct ggcggaggac ttcgcgcgga cggccgccga actcggggag gcgcgctggc
     4021 ataacctcgt gacggactac ctcctcgcgc atccgccgac gcgctggtcg ctccgctggg
     4081 cgggggaggc tctcccggtg ttcctgcgct ctcatccgta cggggcggag cggccctggc
     4141 tggccgacgc cgcgacgctc gaacaggcgc ggaacgaggc cttccaggcg cgcgacgcga
     4201 cgccgctccg gccggaggag ctcgcgctcg tgtcgccgga ggagtggccg gagctgcggt
     4261 tcgctcccgc gcccggcacg gcggtcgtcg cgagccggtg ggacctcgcg ggatggtggg
     4321 acgacgccgc gtcggcggaa ccccccgacg cgctcgagga ggaggcgacg ctcctcgtct
     4381 tccgcgacgc cgaggacgac gtccgacacg aggcgatcgc ccccgaggag gccgaggccg
     4441 cacgcctcct cctcgccggc gcccccttcg cggaggtctg cggcgccttt gccgacgccg
     4501 cgactgacga gaccgacgcc gcccgccgcg ccctggccct cgtacgaagg atggggtctt
     4561 gtctacactc tacactccgc gggaacgcag agtgtagagt gtagaccaga ccccatctca
     4621 tggagaggtt ctcgaggcgc gacgggcggt tcgtggcgct ctgcgtcgcg gtgatcgtgg
     4681 cgggggcggc ggtgggagtg ccgctcttcc cccgggcgtt cccggaggcc tcgatcgact
     4741 ttcgcgtgac gcgggaggag gctcagcagg ccgcggagcg ggcgctcgcc cgaaggggat
     4801 tcgacctggc ggggcggcgg gccctcgcga tcttcgatca cgacgacacg gcgaaggtct
     4861 tcctcgagcg ggagctcggg ctcgagcggg cgcggtctct cctcggggga gacgtaccgg
     4921 tgtggcgatg gtcgttccgg ttcgtgaagc cgctcgagaa gggggagctc ctcgcgttcg
     4981 tggcgccgtc gggggagctc ctctcgttcc gaagggtcct tccggaaggg gcgcccgggg
     5041 gcgatccggg gtccgcccgg gcgcgcacac tggccgagga gacgctccgc gagcaccggg
     5101 ggctcgacgc cgcgacgctg aagctcgtcg aggccaggga ggagaagaag cccgcccgcg
     5161 tcgaccgcac cttcgtctgg gagtcgccga ccgcctcgtg gaagggcgcc gcgatgcgct
     5221 acctggtgga ggtgcagggg gcccaggtcg gccggtcgac gctgtggctc gaggtgccgg
     5281 aggcgtggcg ccaggagtac gcgacgctcc gctcgaagaa ccacgcggcg ggggcggcgg
     5341 cgacgttcgg gctcgtcctg acggcggtgg cgctcgtggc ggcgttcttc gggcggctgc
     5401 gccggcggga cgtgccctgg cgcacggcga tcggcttcgg gctcgcgggg gcggccctcc
     5461 aggcggcggc gtcgctgaac gagctgcccc tcaccctctt cgggtacgac acgacggaga
     5521 gctggggggc cttcgtaggt caggcggtcc tcgcggacct ggggagcgcc agcctcctcg
     5581 gcgtggccct tctcctgctc gtcgcggggg gagagcccga gtaccggcag gcgtaccccg
     5641 acaagccggc gctcggacgc ctcttcacgc gccgggcgtt cgggacgaag cggttcttcc
     5701 tcgggctcct cgcggggtac gcgctgacgg ccggcttctt cgcctggcag gtcctcttct
     5761 acctctcggc ggaacggctc ggcgcgtggt ctccggccga cgtcccctac tcgaacctcc
     5821 tcgggacgat gttcccctgg ctcggggtcc tcctgatggg cttcgtcccg gcgacgacgg
     5881 aggagttctc gtcgcggatg ttctcgatcc cgttcctgca gcggttcgcg ccgacgtggg
     5941 tcgcggtcgg cctgccggcg ctgatctggg gcttcgccca cgcgggctat ccgaaccagc
     6001 ctttctgggt gcgcggggtg gaggtggggc tggcgggggt cgtcgtcgga gtggtgatgc
     6061 tccgccggga cctcttccct cttctcgtct ggcacttcac cgtcgacgcg gtctacacgt
     6121 cgctcctcct cgttcgctcg tcgagcccgt acttcgtcgt ctcgggcctc ctctcggcgg
     6181 gcgcgctcgt ccttccgctc gtcgtggccg gggcgctcgc gtggcgccgc ggcggcttcg
     6241 tgccggagtc gggcctgacg aacggcgacg tcggctcggc gccgccggtc tcccccggcg
     6301 cggccgacga ggctccggcc ttcgcggagc cgaggccgct tccgtcgcgg gccgtcgtcg
     6361 cggtcctggc gggcgcctcg gctctggtcc tcgcaggcgc cgccgcgttc ctgccgtccg
     6421 ggaagctcga cgcggcggcc cgcgtgactc ggacggaggc gctcgacgcg gcccgcgcgt
     6481 tcgtccgcgg gcagggagac gacccggcgc gctacctcgt gacggcgcgg atcgactcgg
     6541 ccctccccgc gctcgaggaa gcgagcgaga cggggcgcgg gctccttccg tatcgctggg
     6601 agcggagcgc ggagcgctgg cttctcgaga aaggcggccc ggagacgctc cgccgctggg
     6661 ccgggggcgt cctccccggc ggggtctggc aggtgcgcct cgcgcgcgag cgggaccgcc
     6721 acgcgtggtg ggtcgtcgtg gacgcgcgcg acgggcgggt cgtctcgttc gcgcgcgacc
     6781 tcccggagga ggaggcgggc gccgcgctcg cgccggacgc ggccctcggg agggcgaagg
     6841 aggcggcgcg ggcgcgcggc gtcgatcccg acgggctggc cgtcgtctcg gcggtcgaga
     6901 aggagcggcc ggcgcggaga gaccacagga tcgtcttcga gtcgacggat gacgcggcgg
     6961 gcgaggcgag gcgccgcgtc ctggtcgacg tcgcgggcgg gaggacgagc ctcgtggcga
     7021 ccgcgctgaa gctgccggag gaatgggtcc gcgcgtgccg ccggagcacg ggagcgacct
     7081 gggccgcgct cggcgcgaag gtcgtcgggt tcgggacggt cgtcggcctc ggtctcgtcg
     7141 tcctcctcct cgcgcaccgt ggaggccgcg tccgctggcg cgaggcatcc cgctgggcgg
     7201 cgctcctgac gctcccggcg cttctcgagc gagcctcccg cctgccgacc ctgctcctcg
     7261 cctggccgcc ggagactccg ctcgccgcct ggtcggtgtc ggcagcgctc ggaatcctgg
     7321 cgggcctcct cgtctcgtac gcgctggcgc tcgtggcgtg cggtctcgtg tcggcggcgg
     7381 cgccccacgc gctcggcctc gcacgccgcc ggtccccggg agcacgccgc cgcgcgctcg
     7441 tcgcgggagg ggcgacggcg ctcctcgtcc tctccgcgcg tcgtctcgcg gacggcctgc
     7501 gcggcctctt cccggcctcg gcgggcgtcg ggggcctcga ccttccggcc ggcgtcgacg
     7561 gctggctgcc ggcggcgtcg tcgctcgcgt ccgcgacgga gctcgcgctc ctcgcggcgg
     7621 gcggcgccgc ggtcgcggcg ctcctggcgc gggacttccg ccggtcgcgc ggcgggtggg
     7681 tcctcctcgg cctggcggtg acggggtgct gggcgccgct cgaggcgtgg gacgccgcgg
     7741 aggtcgtcgt tccgctcctc gtcggggcgc tgccggcggc ggcgctggcc ctcggggccg
     7801 cgctcctgct caaggacgat ccccgggcct tcgcgacggc cgcggccgga atcgtcctcc
     7861 tgccggaggg ggcggcgttc gcggcgtcgg gagtcccgtc ctgggcgtgg agcggggggc
     7921 tctgcctcgc ggccgcgatc ctggtcgcgc tcgcgccggg tcggaaggac gccggcgcta
     7981 ttcccccgag gccttcgccc cgcgcgggct gacgcgacgc cactccgatt cggagaggcg
     8041 gtcggcggcg ccgcgcccga cggcgcggtg catctcgacg aggaggcggg cggcctcctc
     8101 gtcctcgagc ggcggaggac cgaggcgcgt gaggagagcg tccgggtgct cgggcgagag
     8161 gaagcgcgtc ttgagcgtcg cgagcggaac gagcgggcgg tagaaggcgt cgagccgcac
     8221 gtcggggagc ggctcgagcg gcgcggccga ggccgccgcc gctcgagccg ccggggcggg
     8281 gggcgcggcc ggggcggcac gccgggtgcg ggcctcgcgc agtcgggcca ggacctcgtc
     8341 ccgtcccttc cctctgagga ggagaagaag gaacgggtcg cggtcgaatc gttcggcgag
     8401 ggcgacgtgg acggccgcgg cgtggcggca ggcggcgccg tcggggcagg cgcaggtgac
     8461 ggcgacctcc gtcggggaga cggggaggag cgcccctccc gcggccgcga agacgtcgtc
     8521 ggattcctcg gggagaaggc ccgagaggag ctccgcggcg gtgcgggccc gctcggcgaa
     8581 ggccttcagc gcgccgtcga agaccttctc gttcagcgtc cggaggcgca ggcggacgtg
     8641 gaggacggac cgcccccggt cgcggacctc cgcctcgacg aggccgggct tgaccgagag
     8701 ggcggtgagg gctccttcgg cgacgagcga gcgccccttc tcgaggcgtg ccttgcccgc
     8761 gccggaggcg gcggcaagcc agcgccctcc ccagccctcg ggggcgggcg gagcggcggg
     8821 gaggtcgacg aggcgcttct tcgccgacac gtcagctccc ttccccggcg tcgacggcgt
     8881 cgcggctgag ggccacgagc tcggccagct cctcgttgga gagctccgtg atccagctct
     8941 cgccggctcc gatgatggag cgggcgagct ccttcttctc gtcgatcatg tggtcgatcc
     9001 gctcctcgag cgtcccgcgg cagacgaact tgtggacctg cacgtgccgc gtctggccga
     9061 tgcggaaggc acggtcggtc gcctggtcct cgaccgccgg gttccaccag cggtcgacgt
     9121 ggaagacgtg cgaggcgcgc gtcaggttga gccccgcgcc gcccgccttg agcgagagga
     9181 cgaagaacag aggcggatcg tcgtcctcct ggaagcggcg gaccatctcg gcgcgcgcga
     9241 gccgcgggac gccgccgtgg aggaagagga tctccttgtc gaacttgtcg cgcagcgcac
     9301 ggacgaggag gtgccccatc tcgctgaact gcgtgaagag cagcgcgggc cggttctcgg
     9361 cctcggtctc ctcgagcatc gcgaggaggc gcgtgagctt ggacgagcgg ccgtcgaggc
     9421 ggctgccgtc gccgaggaag agggccgggt ggttgcagat ctgcttgagg cgcaggagga
     9481 gcagcaggac cttcgcccgc cggctctgcc cctgggcctt tccgatcccc tcgaggagcg
     9541 ccttcgtcgt ggcgcggtag agggtcgcct gctcgcgcga gaggccgacc cactccttcg
     9601 tctcgatctt gtcggggagg tcgggggcga tcgcggggtc ggtcttcgtc ctgcggagga
     9661 ggaacggaga ggtcaggcgc cgcagccgct cgcgggcgac ggggtcgtgg tgccgctcga
     9721 tcggaatgga gaacgtgcgg cggaacgact cgtcggagcc gaggaggccg gggttcagga
     9781 agtcgaggat cgccttcagc tcggagagcc ggttctcgag cggcgtcccg gtgagggccg
     9841 cgcggcgcgt ggagcggaga gcgcggacgg cgcgcgactg cgccgtgccg gggttcttga
     9901 tgttctgcgc ctcgtcgagg gcgaggtact cccaggtaac gtccgtgagg agggcgcggt
     9961 cgcggtgggc gagggagtag gtcgtgacga ggaggtcggt cttcttcacg agggcgaaga
    10021 aatcgtcgcc ggcggcccgc tccggcccgt ggtggaccgc gacggtgagc gtcggggcga
    10081 agcggtgcgc ttcctggacc cagttctcga cgacggacgt ggggcagacg aggagcgagg
    10141 ggcggatcgc ctcgcccgcc tcgcgggcgg tgaggagcgt cgccaggagc tggatcgtct
    10201 tgccgagccc catgtcgtcc gcgaggcagc tgccgagccc ccgggagagg aggaagcgga
    10261 gccaggtgac ccccttctcc tggtagggac ggagccggcc ctcgagctcc ttcggcgtga
    10321 cgaagggggc gtccttctcg gcgtcggcct ggaggaaatc ggccagccag ccgtcggccg
    10381 tcaggacgtc gacgcccgga gcggcgtcgt cgtcctcgtc gaggccgctc gccattcgga
    10441 ggaagtcggc gagcgaggcg cgccccgagg gcttcttctc gaacagggcg agcgtgcgct
    10501 ggacgctctg cgggtcgagg aggacccact cgccgcgcac ctcgacgagc ggtaccttgc
    10561 ggctggcgag ctgctcgaac tcctcgggcg agaggaggag gtcgccgacg gcgaccttcc
    10621 agtcgaagtc gacgagggtg ccgaggccga accgcgtgac ggcgacgccg ctcttccagc
    10681 ggctctccgc ggcgttgagc cggagcgacg gcttcgtcgt ccgcccctcg gccggaagga
    10741 ggagctggac gcccgcctcg gagaggagcg gggcctcggc cgagaggaag cgccaggcct
    10801 cctcgagcgc gagaggggct ccgaccgggt ggcgctcctc gaggctgcgc cggatcggct
    10861 cgcagagctt cgcgacgccg ccgaggcggg cgaggagcgt ctcctgaggg ttcacgaacc
    10921 gccggccgag gcgccggagg ctcgaggccg tctcgttcca gatctcttcg gcgtagagct
    10981 tcaggctggg gtcgtccgcc gcctcgaggt ggtagccgag gcgccaggag ggcgcaccct
    11041 cggcttccgg ctcggggggg aggaggcgga ctccgaggcg cagctcgccc tcgggcagcg
    11101 cctcgaggag ggggatgctc caggcgccga agcgctccgc gacgtcggcc gggacggcct
    11161 cggcggcggt ttcgtcctcg cgcccgtcgc ggcggacggc gagtcgcgcg agcgcgagcc
    11221 cctccggccc ggggagaccg cggcggcgcg gctccggcag agtctcggcg aggagggccc
    11281 tcgcggcggc gtcggtcagg tcgtcgacga cgccgcgcag gaggaatgcc gcggccgggt
    11341 agaggccctc cgaggcggag ccgggccacg cgatcgcccg gctcgcggcg ggcatcgcct
    11401 gggcgaagac ctcgacgcgc tccttctcgt gggggtcggt caggaccggg cgccagcggg
    11461 cgtgcccgtc ctcgacggcc gggacgaacc gcctgcggtg gaggaggtcg aggacccagc
    11521 cggcgacggc cgaccagtac tcggtgtcat ccccgaggga gaggccggcg cggtccggtt
    11581 cctcggcgag cgagagcagg gcctcgaaag cgcgctcgga cgggacgagc agcccctcga
    11641 cctccagcgg cttcaggacc gtctcgccgg ccgcgtaatc gccgtcctcg cgcagggagg
    11701 agcgcgtggg gacggggcgc gagcggagcg tcgggaccgt caggacgagg cggagcggcc
    11761 gggcgcctcc cgtggcgacc gcgagcccgg ccgaccggag cgccgcggcg aggagggacg
    11821 ggtcgacgtg ccgggggtgc tcgggacgct gccgggcgcc gacgaacggg ccttctcccc
    11881 agaggaagaa tcccgttccg gggagtggcc ccgtcggggg cagcgggttg ccgtggagta
    11941 cgagcaagcc gcccattctc gcgcatttcg actcgggcgg cgcgaccggg tatcctccgc
    12001 ccctgcaacg atgaagccgt acgagatctt ccgccgcctc tcgaccgccg aagccgacgc
    12061 cctcgtcctc tccgcctgcg aggacgacga gatccccgac aaggtcgccg ggagcgtcct
    12121 gacgctccag cgcgtgccgc tcaagcgatt cgagcggatg acggacgagg tccggcgcgc
    12181 cctcgtgagg aagaccctcc gcgaccggcg ggccgaagac ctcgcgctct tcgtcctgtc
    12241 ggccggcctc gtgcgggagc ggggcgagat gatcgagggc ttcctctcct cgctcgacct
    12301 gccgcacgac ggaccgaacc tcacggccga cgggccggtg gcagagcccc cgcgcgagcg
    12361 cctcgtgaag gccgtggcgg acctcgccgg gaagcacggc gcgcgggacg cggcgctctt
    12421 cctccatgcc ttcgtcgagc agccggacgt ggcgtggccg gtcctcgcag ggctcctcga
    12481 cggcgacccg aagctcgccc tggaagaccg ctccgcagac tgagccgggc gcccggcggg
    12541 acggcggcgt ccggccggac gtcggctaac atcctcgtgc gcccgcgccc gaaggcgcgc
    12601 ggccggaggt tgaccatgat ccccgagaag ctgaaggccg ccctcgacga gcacgtctcc
    12661 gcggaggtcg gcgctgccta cctctacctc gccatgtcgg ccgacttcga gtcgaagtcc
    12721 ttcaagggtt tcgcgcggtg gatgcgcatc cagttccagg aggagatggc gcacgcgctg
    12781 aagttcgtcg actacatgct ctcgcgcggc gcgacgatcg gctggaagga cgcaaaggcc
    12841 ccggcgagca cgttcgggtc gccgctcgag acgttcgaga agaccctcgc gcacgagaag
    12901 gacgtcacgg cccggatcca caagctctac cacctcgccg cggccgaggg cgacaccgcc
    12961 acgcaggtct tcctgcagtg gttcgtgacg gagcaggtgg aggaggaggc ccgcgtcacc
    13021 gagatcgtcg acaagatccg gatggtctcg gaccgccccg gctccgtcct ctacctcgac
    13081 aaggagtacg gcaagcgcca ggcctgaggc cgcactcctc ccggttcgct gcgcagtcag
    13141 ggggcgccgt cggcccgggc cgacggcgcc cttcgtccat tcggaccggg aggagcccgg
    13201 cggcgagcag cggcccccgg cccgcagact gtagaacgta gaccagaccc ctcggcctcg
    13261 gacccctcgg cctcgagccc gctcgccccg ggcgcgagcg ccgcgaaggg gggtgggcgg
    13321 ggggagcggg gtgggggacg cggcagatca gctgccggtg gagccgtacc tcgcgatgcc
    13381 cctgcgccag agggcgacgt tgaggagggc gaagacgacg gcgacgagcg ggacggccca
    13441 gaagtacgga aggtactcgc cccgccggag cgcgagggcg gtcgggtaga acgaggcgaa
    13501 ggcgaacggg atgacggtgg aaaggaggag cttcaccttc aggtcgtaga tcgtgatcgg
    13561 gtagcgcccg aacgacatga ggttgaagac ggggggcgcg aggccgaccc ggtcctcgac
    13621 ccagaagctc gcggcggtca ggagcccgaa gaccgagacg tagaggaccg ccccgaagac
    13681 ggcccagagc ggaaggagcg cgaggccgag gggcgagacg tcggggagcc gcgacacggc
    13741 ccacccgatc gccacggcgc cggtggccac ctcctggagc gactcgaggc ggaagctctc
    13801 gaagaggacc tggaagagcg gcgagacggg gcggaggagg atgcggtcga agcgcccttc
    13861 cacgaggtag cggtcggcga actcccagag gttcgacgag acgacgctga agagcccgta
    13921 cgggaggagc gagaagccgt agaggaagac gatctcgggg aaggtccagc ccgcgaggtg
    13981 cgggaagcgg gagaagagga ggaggacgac ggagaacgac gcggccgtgc cgaggaacga
    14041 ggcgagggcg gccgcggcga aatcggcccg gtaggcgagg cgggccttcg cgtactgggc
    14101 gaagtagagc gcgaggaggc gtgcgtggaa gcggacgcgg cgcaccgtca gcccccctgg
    14161 acctcgatgc ggcgcgccgt cgccttccag aggaggcgcc cgagaaggag gagcgcgacg
    14221 acccacgcgg cctgcaggcc gagggcgcgc gcggcgtcgg cgccggtcgc gaggccgagc
    14281 cagatgcgcc ccggcgtgtc gttcatgtgg aggaacggga gccagacgag gaccttcctc
    14341 accggctcgg ggaagaaggc gaccgggacg aggagcccgg agaggacctc gaggacgagg
    14401 tacttcgcgc gcaggacgcc gaggatcgag gtcgtccgga tggcgacgag cccgacgcag
    14461 aagttgatcg ccccgacgag gagcgccgag aggagtcccg ataggaggta gagcgcgagc
    14521 gcggccggcg aggcgggcgg gaggacgggg tagacgacga cgaggaggag gccgatcgga
    14581 agggtgaaga ggacgaggcg gaacgcgctc tcgccgagcg cctgcgagag cgtcatcgcc
    14641 tggacgtcga ccggcttgac gagcgtcatc gcgagccgcc cctcgcggac ctggctcgcg
    14701 atgtcgcggt cgacctcgtt gaagtagaag ctccggagcg cccagccggt cgcgacgtag
    14761 gtgagcatct gcgggaacgt gaagccgccg agcgtcgcgc cctcgccttt ctcgtggaag
    14821 agcgccctcc agatgtagta ccagacggtg gcgttgaaga agtaggtgag gacgccgacg
    14881 aagtagcgcg cgcggtaggc gaggatgttc aggaacgagg tccgcgcgaa ggcgaggtag
    14941 gggcgcgccg cggcggcgag agcggtcacg cgctcacccc cggcggacgc cgcggtggga
    15001 gacgaagccg ggcgcggccc cctcggacgg aggcggggcg tcgggcttcc ggagggcggc
    15061 gttctcgcgg tagatgcggg agacgacctc ctcgatgtcg ggctcgctga cggcgaggtc
    15121 gaggacggga aggccgcgga cgatctcgcg gatgacgtcg gcggaggagg cctcgcgctt
    15181 gtcgaccgcg aggaggtagc ggcccggcga gaggcggccc gccgtccagg gcccgatgac
    15241 gaggtcctgc gggtcgcccg gcggcggctg gggaaggtcg aagcggatgc gcgcctcggg
    15301 gaggtgctcg tcgcgcaggc cgcgcaggct cccgtcccag aggacctgcc cgtggtcgat
    15361 gaggacgacc ctctcgcaga gctcctcgat gtcgtcgagg tcgtgcgtcg tgaggatgac
    15421 ggtcgtctcg aggagcttgt tcgcctcctt gaggaacgag cggacgtttg ccttggcgac
    15481 gacgtcgagg ccgatcgtcg gctcgtcgag gaagaggagc gggggctcgt
//
LOCUS       DAOTEZ010000048        15500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2603_length_15500_cov_5.249531, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000048 DAOTEZ010000000
VERSION     DAOTEZ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15500
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2603_length_15500_cov_5.249531"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..2304
                     /locus_tag="P5164_04020"
     CDS             199..2304
                     /locus_tag="P5164_04020"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRY43094.1"
                     /translation="MILFAIASVPLSALALTDVAGTISQSTTWTLAGSPYVVTGWVYV
                     RGASAPVLTIEPGVEVRFNGGQGMSIGYEQPGGLVAVGTPEKPIRFTSNAATVAPRHW
                     RGLFFDPLATAGSRIENAVIEGAGSNGEAGTTVSGSAPTFQSVTVRTNGAAAIRVNGG
                     APTIAGCILQGSRDWDAVGLDAHGGTVTLRDTTITGNAGAAIALWAGAALEGLTGLTV
                     KENPGGNVVRYRSTEIPTSQTWKSFGLPYVLMDGWVHVRGAGSPVLTIEPGVEVRLAG
                     GHGLSIGDGSTPGGLVAVGTAEQPIRFTSHAASVTTGHWGRVGFSAATVAGSRIENAV
                     VEGGGYHWDGAVVVYGGTPAFWSMTLRKNRGSGLRVDGGSPTIFGCEIREGTERGLWL
                     SGGSLPRVYGNTITGNAGAGIQNESSRATIRFNAVSGNGGYGILSSNGTFFARDNSVT
                     SNGAPARNDDSASRTLDARQQWWGAETPPAGLVGRVQYDPWLGSPPTPPFAVSSFERS
                     TAAFNPEGAGVTFAFDLPSPGAWTLTLADAEGTTVRSFSGNALSAAIRWDGSDEGGLS
                     LPDGVYAYRLTAVEPISSIAAAPLLGRVTLDRTLPLALLAAPTPRAAFVSPVGVSGTA
                     AGSGFASYTLEYGAGEHPATTTLIDRGSLPVTEGSLGLWSALLTEPLYTLRLTVTASG
                     GKVATQSVTVRLDGQTACQ"
     gene            2313..>7147
                     /locus_tag="P5164_04025"
     CDS             2313..>7147
                     /locus_tag="P5164_04025"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRY43095.1"
                     /translation="MKRRFFLGLLIALLPATALRADNGTLGTGQYRLIGASFDVSPVE
                     QNVPVGVAATVKTLFEGSASALASTGARVAAELTGPSVPSPVTISAAPGEDLVVPPLS
                     VKGEHLLQNIRLTNSGGVAVAAAHPTARIVVSDILVTRITSRALTSQELAARGVVVND
                     SSYKAYSFALGLAIQGRTIRIEVPTVVETRDGYQPVGPPIVDVENRDERFVPPTVIAV
                     PMRKDGTPGGGPPDPEIFDEGEGSPQPVFGLLVFPGNIRFLNQFFSVVVMVQNGAAEG
                     SPLALRDVSTTISLPAASLRLSKTIPSVPDGSPVPVRGAGADEVLGTADDVVVLVAQT
                     AGQAEFVTEGLRVGTHEVTCDLSATLEGLANQPPRTLRGRARGSVLVRDPSFGLTFNH
                     PDVIRDGEEYELRVSVANTSTVLANDVTIAIDAASLTGAEALGVPAGTDPAALLGDIP
                     PGESRLAKFALKAKRTGRVVASAFTSDGPVVGSLRLRTGVTNDGIPLSPDSFIFPRVI
                     SVLPPEVVDPATSLIGIAHGLATMDPTAPGVGCGLLGDDCAPPFADGVVQDRVFDLVT
                     AARRASLGEPVAAAVADLAFSWLGSAVPSPGFDGVRRSNVHGSDFEAGVGAVLSAHAE
                     EAGIAGLERTLLDAAVATRAPTNPLAGPTWALLEEGGSADPAGRLYIGDPAAGTLSGL
                     RPGEPGYVREAAYTSLHGIGGPVGGELAIAARPPDAGVVFGIEGKRADTVALTVFLAD
                     GAGGFRRARIAGIVTSPGSVTNVHVVSGATWLQAVSATDVLRNADAVAVEPSPFGPYA
                     AIQDVAANPLGKAVSLVFNRPLREAEAAQVQRWALPAPRPDGGTYERTVGGAFPRDSD
                     PRYLTLVSETVVSPFRPGEAVGAGIPALDGGVWSGALPIIPRLVQPGGTVVGRVIGPD
                     GTPLPNAPVRLSESATDDLTGEVFAATTSVTRTDTTGAFYFDYVRKQDGKPFRVDSFD
                     AITGSKGYAVGSVRTQGQTVQVDVVLQGRGTVKGTVVDGTGAALEGVIVRCASDNDNG
                     FRTAQLSHSDGGFTFTSVPVGTVRLQAEDPATNRTAYATVGLSAPGGVAERQLVIAQL
                     PRTSLRGVVRHGRDGSPYAGVHVAGYGQLGEYFGARQTDAEGSFSFESAPVGNVRLEL
                     FDVFVSRSPILVQTVTLVADQPAEVTLTIEEAPARFGAVAGTVRKVVGGAPIPAPGIV
                     VFVQSSGHRTTTAADGTYRIEDVPIGAQTVTALDPATGRSVSGSATVQHELTSPLDLQ
                     FADGTLGRVVGTVVDQDGRPKGGVVVEIVDEGPPVTVPFRSATAADGSFTLQNVPPGT
                     YRVQATFGEVRGGRTLRNAGAAPVTVPGPGTAGQAAIQLRGWVTVSGRVVARVRDRNG
                     VLHDNPVFAPVELHTARFADGTSNPDPEAGPIYRDGPSLYATVNTDPQTGEFRFEDAR
                     GGPIRVVAKNPFYGERAHAFGFVQGDATRGPVDIVFDGNLGVVDGYLFNADGSPIVGG
                     HLTLTGAGPFDVIEATTRPAGPSNAAGYFVFPLVPFSPRIQVRYSEELGGVPRVAEAN
                     VSLSAQAPTARVTLRALGVGSVTVRVVQPAGDDLVPVAGAQVRVDELEGLFERGDIVS
                     VR"
     assembly_gap    7148..7157
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7303..13035
                     /locus_tag="P5164_04030"
     CDS             7303..13035
                     /locus_tag="P5164_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRY43096.1"
                     /translation="MKLTGTAQVSGVVRSPVDGSPVANVNVLLVSLGGGGLGIGPLAA
                     MTTAPDGSFVFEELPAVPGALYRADAEDSGTLRRGSSATLALAPGDAKQADVTLNALG
                     AVTGRLTTFDGATALEGAEVSVSSFESVPGSEHPRVTSLIATTDADGVYRVDGVPAGS
                     ISVRAHDEITGLSAVGSGTLVSEGQVVRIDLRATPTGSVQGLVRRADGSSLDETSAVP
                     TVSISSHGLSRVVTARSYEFTGIPSTHEFVLAAREPASPFHEATARGSVGAGQTQTVD
                     LRYGPIGSVRVHLRRPDPTAPGSFVPAVGLVSINQGGPYAHRFPSNASFRTDAQGELT
                     IIDVGGGFGLSVRAVEDGTGAGGSATVPDLMLDGQQAEVTVVVEPRGIVRGRVLLPDG
                     VTPASGVPVRLNVAGWLISPFSPPPATVSVIAGPDGRFELPDVPLQSYFVIAEATEPP
                     SKVYVEGSISRSDLVADLGDLVLDDARPALVSVEPPEGTAELGLTPTIRFTFSEPLWP
                     YGDSGLWNLVRVTAPTGDISTGFRASLDATGQILTVQPKYPLPGATLYEVRVSGNLRD
                     RNTLATGVDAVTRFTTADLTNPVVLRTTPVADQIQVSVLVNPSVVLSKAIDPATVVAG
                     TRLYRLDAAGSSVAISPALQADGQTIVLNPEASLEAEAEYEIVVDALKDIAGNPLLAI
                     TRVRFFTRDDAAPLLAFDPPDTPEPIEGTTRSYTVRFGDDDVETATLYLIAPTGEIWN
                     ATKSVVSPARDARSITWSVRLPRVAEAGGTAVRCRAFAVDFCGNSSAPVDLPLTLQID
                     SLPTLVSAEASTATVRIGGTFTVAVSATDDLSLAGIDASPSAGIEAVSAEVTASSPTS
                     RTELRTYRVRLDAAPGAASVSFVARDSRGQLSPATSVSVTVQPDDAPVVSILSPASGS
                     SVLSGASLPVTYSATDDLGVVSLVVRLGESSVDVPSPAATGTVNVRVPVVTTVGAHDL
                     TVTATDSTGHAVSATVSLTVRPDEPPSVLISAPPAGATLVSGAGVPVTYTVSDDVGVV
                     TLLLRLGDSQVPISNPSGTGTATLVAPVVAAAQSLSLTARATDASGHVAETSVAVTVK
                     PDDPPAVAILSPAVGTSVLSGATVPVAYSVSDDVAVASLELKLGSSSVMLVRPPAVGT
                     TNLTAPVVTAVEPVVLSATATDSAGHVVPTSVTLSVRPDEPPTFTVTAPTEGLHVKSG
                     ASLLVSGSLADDNGRATLTATLGPASRSVASVGPFSFTLPVPVVAVSTPITLALRAQD
                     FTGHTATPQDLGLVVEPDTVSPTIVVSSPAPGSSIVGGKAFALVATASDDVAVTGFRY
                     RVDQGAFVPVAGRVLSVQVLTGTVSVPTPMTIEVEAEDGAGHVTPSTVGLTVVPNQAP
                     TLSLTRPAAEARIPAGATFAVEGTSVDDSGVPTTTVTFDGQTRSATSNGFAVTFTAPF
                     VSTETTFTLSATARDPEGNESPEVSRSLTVVPDVGGTPTVQLTAPAPGVLLVGETAAI
                     SITFADNVGLSFGSAEVTGPFTSGPRTWSLSGTSSVQSVEIAPADRPFGEKARVVAKN
                     VDLGGRVANLDVSIPVAYHRLELPLPSGALAEGSTLTAVFRIGESGRQRASALRLEIG
                     TVSGQTFTVLACVQTAAPLSELETLAVRVPVGRTGLFVRSILVETTGDLAQAAAFDGT
                     AVLLEPLSTSGDTAAPTVVIMNPADGLSVLSGDLVQTSVSAADDTGVERIDASFGGIT
                     KTCSAATCELTFFAPVVATAGTQTLSATARDGAGKSTTTSVAVTVSPRSSGTTSGPEP
                     GELVAGDGRAPIVRFLTPHVSPYPVAPNAPFRPAVEAVDADGIDRIELFLGETDVPCL
                     VLRPDLPSRDACFVPDAAAGSTLWLRAVAFDLSGDRSEVEVELVVTPGIRIPEDDVSP
                     AEPDRPEETE"
     gene            13036..13242
                     /locus_tag="P5164_04035"
     CDS             13036..13242
                     /locus_tag="P5164_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43097.1"
                     /translation="MEPQTLLDRSLEASASVVFGGDGAVAASEAPLVLLATRDPFLDS
                     GGVGSAGTFLVHGAAHPHSSLEQP"
     gene            13239..>15500
                     /locus_tag="P5164_04040"
     CDS             13239..>15500
                     /locus_tag="P5164_04040"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43098.1"
                     /translation="MKPTRLVVLALLGLLVAVPPAAAQQDPFAIPQALSGQAVFSAQA
                     LTVNGGAVVDSSGVAGGSGAGSEGHVVSNSDITLNGKSKVKGSAWYGPGKKIVLNGGS
                     TVSGTTAALAAARDAAPIALSGLAATLAASNDNALIGKTSRNRSPLQGSGGTDFVLNG
                     NETITIPAGTFYFTTITINGSSKLSFSGLTRILCTGPVTVNGTSTVHQNGNPWLLRLW
                     VSGAQVTLNGNSTLNGFVYSPSAAATVDGGGILTGGLFAQKVTVNGGGRVTRSFDDTS
                     ALAATVTNAGQPIANGAVFRAEVAPVVTVTGGVPPLATRVTLDGAPFVSGTTVSAEGV
                     HRLVVVSDDLAGRHLELTVGFVIDRSGPSLAIVRPANGEVVGTAPVDVTGTSGDATSV
                     SVNGVAGTVAGGSFVVRGVPLAEGTNTLVAAGADAAGNAGSATSVVVLDTGAPVLTVA
                     SPADGLLTKAETALLQGAVSDPHLTSLTVNGLPISVPSGGGAFSREVALVEGANALTV
                     EARDVVGHIASRSLTVRRDGVAPEIRIEEGNLPLEAGRAYNRDLTIRVTVVEANPGTL
                     EVTLDGATFVSGATVSAEGAHTLSATAVDGAGNSASKTVSFSIDKTAPSLSITTPTPG
                     STILDLPRTLAGTSGDAVTVDVDGTVVAPAGGAFSIPAFPFAEGPVTVTATGTDAAGN
                     SGTATATYLVDTLPPQVTIASPLEGVRLGAAPIFVTGVASDANLLEVRVNGQAASLGS
                     DGSFQAGPFRPADG"
BASE COUNT         2079 a         5677 c         5405 g         2329 t
ORIGIN      
        1 gagcttatgc actgtgcagc gaacgctctc gtcgtctcgg agcgcagacc gatgaataca
       61 gcagtcacct ctggatgtct ccgggggcgt atcacgagtc gtgagtgtga aggtgatgcc
      121 gagcgccgga ctcgagccgt tggtgagtcc cgctcggcgg tctgcgcgaa ggcgtgcctg
      181 gtcacgacgg ccagctgggt gatcctgttc gcgatcgcgt ccgttccgct ctccgccctc
      241 gcgctgaccg acgtcgccgg aacgatctcg cagtcgacga cctggacgct tgcggggagt
      301 ccctatgtcg tgaccgggtg ggtctacgtg cggggcgcgt cggcgccggt actgacgatc
      361 gagccgggcg tcgaggtgag gttcaacggc gggcagggta tgtcgatcgg gtacgagcag
      421 ccgggcgggc tggtggcggt gggtacgccg gagaagccga tccgattcac gtcgaacgca
      481 gcgacggtag cgccaaggca ctggcggggg ctcttcttcg acccgctggc gaccgcgggg
      541 tcgcggatcg agaacgctgt gatcgaagga gcgggatcga acggagaggc gggaacgacc
      601 gtctcgggta gcgcgccgac gttccagtcg gtgacggtgc ggacgaacgg agcggcggcg
      661 atccgagtga acggcggcgc gccgacgatc gcgggatgca tcctgcaggg ctcgcgggac
      721 tgggatgcgg tcggcctgga cgcccacggc gggactgtga cgctgaggga tacgacgata
      781 acggggaatg cgggcgcagc gatcgcgctc tgggccggag cggccctcga ggggctgacg
      841 ggtctgacgg tcaaggagaa cccgggcggg aacgtcgtga ggtaccggag cacggagatt
      901 ccgacgagcc agacatggaa gagcttcggg ctgccgtacg tcctgatgga cggctgggtc
      961 cacgtgaggg gcgctgggtc gccggtgctg acgatcgagc cgggggtgga agtgcggctc
     1021 gcgggcgggc acgggctgtc gatcggagat ggatcgacgc cggggggcct ggtggcggtg
     1081 gggacggcag agcagccgat ccggttcacg tcgcatgcgg cgtccgtgac gactgggcac
     1141 tggggtcggg tcgggttcag cgcggcgacg gtggcgggct cgcggatcga gaacgcggtg
     1201 gtggaggggg gcggatatca ctgggacggc gccgtcgtcg tctacggcgg gacgcccgcg
     1261 ttttggtcca tgaccctgcg gaagaaccgc ggctccggtc tccgggtcga cggcggatcg
     1321 ccgacgatct tcggatgtga gatcagggaa ggcaccgagc ggggcctgtg gctcagcggc
     1381 gggagcctgc cgcgtgtcta cgggaacacg atcaccggaa acgccggcgc ggggatccag
     1441 aacgagagct cccgggcgac gatccgcttc aacgcggtct cggggaacgg cggctacggg
     1501 atcctgagct cgaacgggac tttcttcgct cgcgacaact ccgtgacgtc gaacggcgct
     1561 ccggcgcgga acgacgactc cgcgagccgg accctcgacg cgagacagca gtggtggggc
     1621 gccgagacgc cgccggcggg gctcgtcgga cgtgtccagt acgatccgtg gctcggctcg
     1681 ccgccgacac cgccgttcgc cgtcagctcc ttcgagcggt cgacggcggc cttcaacccc
     1741 gagggggctg gcgtgacgtt cgccttcgac ctcccgtcgc ccggcgcctg gaccctgacg
     1801 ctggccgacg ccgagggcac gactgtgagg tccttctccg ggaatgctct ctccgcggcg
     1861 atccgctggg acggtagcga cgagggaggc ctttcgctgc cggacggcgt ctacgcgtac
     1921 cggctcaccg cggtggaacc gatctcctcg atcgccgcgg ccccgctgct cgggagggtc
     1981 acgctcgacc ggaccctgcc gctcgcgcta ctggcggcgc cgactccgag agcggcgttc
     2041 gtgtcgcccg tcggggtctc cggcacggcg gccggttccg gcttcgcgtc gtacaccctc
     2101 gagtacggcg ccggcgagca tcccgcaacg acgacgctga tcgaccgcgg gtccctcccg
     2161 gtcaccgagg gaagccttgg gctttggagc gcgctcctga cggagccgct ctacacgctc
     2221 cgtctcaccg tgacggcatc cggcggcaag gtcgcgacgc agtccgtcac cgtccgtctc
     2281 gacggacaga cggcgtgcca gtgaggagct cgatgaagcg ccgcttcttc ctcggccttc
     2341 tcatcgcgct cctgccggcg acggccctgc gcgccgacaa cggaacgctc ggaacgggcc
     2401 agtaccggct catcggcgcg tcgttcgacg tctctccggt cgagcagaac gtccccgtcg
     2461 gggtcgcggc gacggtgaag acgctcttcg aaggttctgc cagcgccctg gcttccaccg
     2521 gcgcccgcgt cgccgccgag ctgacgggcc cctccgtccc atcccccgta acgatcagcg
     2581 ccgcgccggg cgaggacctc gtcgtgccgc ccctctccgt gaagggggag cacctgctcc
     2641 agaacattcg gctcacgaac agcggtggcg tcgcggttgc cgcggcccac cccacggcgc
     2701 ggatcgtcgt gagcgacatc ctcgtcaccc ggatcacgtc ccgcgccttg acatcgcagg
     2761 agctcgcggc gcgtggcgtc gtcgtgaacg actcgagcta caaggcgtat tcgttcgctc
     2821 tcggcctcgc gatccagggt cgaacgatcc ggatcgaggt cccgaccgtg gtcgagaccc
     2881 gggacggata ccagcccgtc ggtccgccga tcgtcgacgt cgagaaccgc gacgagcggt
     2941 tcgtcccgcc gacggtcatc gccgtcccaa tgcgaaagga cgggacgccc ggcggcggac
     3001 cgcccgaccc ggagatcttc gacgagggcg aggggagccc gcagcccgtc ttcggcctcc
     3061 tcgtcttccc gggaaacatc cggttcctga accagttctt ctccgtcgtc gtcatggtcc
     3121 agaacggcgc ggcggagggc tcgccgctcg cgctccgcga cgtctcgacg accatctcgc
     3181 tcccggcggc gtcgctgagg ctgtcgaaga cgatcccgag cgtgccggac ggctcgcccg
     3241 ttccggtccg gggcgcaggc gcggacgagg tgctcggcac ggcggacgac gtggtcgtcc
     3301 tcgtggccca gacggcggga caggccgagt tcgtgaccga gggtctccgc gtcgggacgc
     3361 acgaggtgac gtgcgacctt tccgcgacgc tcgagggcct ggcgaaccaa ccgccgcgga
     3421 ctctccgagg gcgcgcccga gggagcgtcc tcgtgcggga cccgagcttc ggcctgacgt
     3481 tcaaccaccc ggacgtcatc cgggacgggg aggagtacga gctgcgcgtg agcgtggcga
     3541 acacctcgac cgtcctcgcc aacgacgtca cgatcgcgat cgacgccgcg tcgctcacgg
     3601 gcgccgaggc cctgggcgtg ccggccggta ccgaccctgc cgcgcttctc ggcgacatcc
     3661 ctccgggcga gtcgcggctc gcgaagttcg ccctgaaggc gaagcgcacc gggcgcgtcg
     3721 tcgcgtccgc gttcacgtcc gacgggcccg tcgtcggcag cctgcggctc cggaccgggg
     3781 tgacgaacga tggcatcccg ctctcgcccg actcgttcat cttccctcgc gtcatctcgg
     3841 tcctgccgcc ggaggtcgtc gatcccgcca cgtccctcat cggcatcgcg cacggtctcg
     3901 cgacgatgga cccgacggct ccgggcgtcg gctgcggcct gctcggagac gactgcgccc
     3961 cgccgttcgc cgacggggtc gtgcaggacc gcgtcttcga cctcgtcacc gccgcgcgtc
     4021 gcgccagcct gggcgagccc gtcgcggccg ccgtggccga cctcgccttc agctggctcg
     4081 gctcggccgt gccgtcgccg gggttcgacg gggtccggcg ttcgaacgtg cacggtagcg
     4141 atttcgaggc gggcgtcggc gcagtcctca gcgcacacgc tgaagaggcc gggattgccg
     4201 gcctggagcg gacgctcctc gacgccgccg tagcgaccag ggcaccgacg aaccccctcg
     4261 ccggcccgac ctgggccctc ctcgaggagg gcgggtcggc agatcctgca ggccggctct
     4321 acatcggcga cccggccgcc ggcacgctgt cgggtcttcg gcccggagag ccgggctacg
     4381 ttcgggaggc ggcatacacg agcctccacg ggatcggggg accggtcggc ggggagctgg
     4441 ccatcgcggc gcggcctccg gacgccggcg tcgtcttcgg catcgagggg aagcgagccg
     4501 acacggtggc gctcacggtc ttccttgcgg acggcgccgg gggctttcgg cgagcgcgga
     4561 tcgcggggat cgtgacgtcg ccgggatcgg tgacgaacgt ccatgtggtc tcgggcgcga
     4621 cctggctcca ggccgtctcg gcgaccgacg tccttcggaa cgcggacgcg gtggccgtcg
     4681 agccgagccc gttcggcccg tacgccgcca tccaggacgt cgcggcgaac ccgctcggga
     4741 aggccgtgtc gctcgtcttc aaccggccgc ttcgcgaggc ggaggccgcg caggtccagc
     4801 gctgggcgct ccccgcgccg cggcccgacg gcgggacgta cgagcgaacg gtcggcggcg
     4861 cgttcccgcg ggattcggac cctcgctacc tgaccctcgt gtcggagacg gtcgtcagcc
     4921 cgttccgacc gggcgaggcc gtcggcgcgg gaatccccgc cctggacggc ggcgtctgga
     4981 gtggggcgct cccgatcatc ccccggctcg tccagcccgg cgggacggtc gttggacgcg
     5041 tcatcggccc ggacgggaca ccgctcccga acgcgcccgt tcgtctctcg gaatcggcta
     5101 cggacgacct aacgggagag gtattcgcgg cgacgacctc cgtcacgagg accgacacga
     5161 ccggggcgtt ctacttcgac tatgtccgca agcaggacgg aaagccgttc cgggtggact
     5221 cgttcgacgc gatcacgggc tcgaagggct acgcggtcgg atccgtgcgg acccagggcc
     5281 agaccgtgca ggtggacgtc gtcctccagg ggcgcgggac cgtcaagggg accgtcgtcg
     5341 acgggaccgg agcggcgctc gagggcgtga tcgtccggtg cgcgagcgac aacgacaatg
     5401 gcttccgcac ggcgcaactc tcgcactccg acggcggctt cacgttcacg tcggtcccgg
     5461 tcggaaccgt ccgcctgcag gcggaagacc cggcgacgaa ccggacggcc tacgccacgg
     5521 tcggcctgtc ggccccgggc ggggtcgcgg agcggcagct cgtcatcgcg cagctcccga
     5581 ggacgtcgct tcgaggcgtc gtccggcacg ggcgggacgg atcgccgtac gcgggggtcc
     5641 atgtcgccgg ctacggccag ctcggcgagt acttcggagc gcggcagacc gacgcggagg
     5701 ggagcttctc gttcgagtcc gccccggtcg ggaacgtccg cctcgagctc ttcgacgtct
     5761 tcgtctcgag atcgcccatc ctcgtccaga ccgtcacgct cgtcgcggac cagccggccg
     5821 aggtgacgct gaccatagaa gaggccccgg cccgcttcgg cgccgtggcg ggcactgtcc
     5881 ggaaggtcgt cggcggcgcg ccgatcccgg cacccgggat cgtcgtcttc gtgcagtcct
     5941 cgggacaccg gacgacgacc gcagctgacg ggacgtaccg catcgaggac gtcccgatcg
     6001 gggctcagac tgtgacggct ctcgaccccg ccaccggacg atcggtctcg gggagcgcca
     6061 cggtccagca cgagctgacc tcgcccctcg acctgcagtt cgccgacggc acgctcggcc
     6121 gcgtcgtcgg gacggtggtc gaccaggacg gccggccgaa gggcggcgtc gtcgtcgaaa
     6181 ttgtcgacga agggccgccg gtcaccgtcc cgttccggtc ggcgaccgcc gccgacggga
     6241 gcttcacgct ccagaacgtc ccgcccggca cgtatcgggt gcaggcgacg ttcggggagg
     6301 tccgcggggg ccggacgctc cgcaacgccg gcgcggcgcc cgtgacggtc cccgggccgg
     6361 gaaccgcggg gcaggcggcg atccagctcc gcggctgggt cacggtgagc ggacgggtcg
     6421 tcgcgcgcgt tcgggaccgc aacggcgtgc tccacgacaa cccggtcttc gcgccggtgg
     6481 agctccacac cgcgcggttc gctgacggca cgtcgaaccc ggacccggag gcgggcccga
     6541 tctacaggga cgggccgagc ctctacgcca cggtcaacac ggacccccag acgggggagt
     6601 tccgcttcga agacgcccgg ggaggcccga tcagggtcgt cgcgaagaac ccgttctacg
     6661 gggagcgggc gcacgccttc ggcttcgtcc agggcgacgc gacgcgcgga cccgtggaca
     6721 tcgtcttcga cgggaacctc ggtgtcgtcg acgggtacct cttcaacgcc gacggaagcc
     6781 cgatcgtggg gggacacctc acgctcacgg gggccggccc cttcgacgtg atcgaggcga
     6841 cgacccgtcc ggccggtccg tcgaacgccg ccggctactt cgtcttcccg ctcgtcccgt
     6901 tctcgccgcg gatccaagtc cgctactccg aggagctcgg gggcgtgccg cgcgtcgccg
     6961 aggccaacgt ctcgctttcg gcccaggcgc cgacggcacg ggtgaccctg cgcgcgctcg
     7021 gggtcgggtc cgtcaccgtt cgcgtcgtcc agcccgccgg ggacgacctc gtccccgtcg
     7081 ccggcgcgca ggtgcgcgtc gacgagctgg agggcctctt cgaaagaggc gacatcgtct
     7141 ccgtccgnnn nnnnnnnggc gacgcggacg acggactccg acgggatcgc gtccttctcg
     7201 aacgtcacag agggccggat cgccgtcctc gcgcgctcgg gcctcgtcgg aggccgcgcc
     7261 tccgtcttcg gctcgggaga gggcttctcc gtcgacgccg tcgtgaagct gaccggcacg
     7321 gcgcaggtct cgggcgtcgt ccggagcccc gtcgacggct cgcccgtggc gaacgtcaac
     7381 gtcctcctcg tctcgctcgg gggtggaggg ctcgggatcg ggccgcttgc cgcgatgacg
     7441 accgcgccgg acgggagctt cgtcttcgag gagcttcccg ccgtgccggg agcgctctac
     7501 cgcgccgacg ccgaggactc gggcacgctc cggcgcggga gctccgcgac gctcgcgctc
     7561 gctccgggcg acgcgaagca ggcggacgtc acgctgaacg ccctgggtgc ggtgacggga
     7621 cgcctgacga cgttcgacgg ggcgacggcc ctggagggtg ccgaggtctc agtctcctcc
     7681 ttcgaatcgg tcccggggtc ggagcacccg cgagtgacgt ccctcattgc cacgaccgac
     7741 gccgacggcg tctaccgggt tgacggcgtg ccagcgggct cgatctccgt ccgcgcgcac
     7801 gacgagatca ccggcctctc ggccgtcgga tccgggactc tcgtttccga gggtcaggtc
     7861 gtccggatcg acctgagggc gaccccgacc gggtctgtcc agggtctcgt ccgccgggcg
     7921 gacggttcgt cgctcgacga gacctccgcc gtaccgaccg tctccatctc ctcccatggc
     7981 ttgagccggg tcgtcacggc gcgctcgtac gagttcacgg gcattccctc gactcacgag
     8041 tttgtcctcg cggcccgcga gccggcgagc ccgtttcacg aggcgaccgc aagaggctcc
     8101 gtcggcgctg gtcagacgca gacggtcgac ctgcgctacg ggccgatcgg gtcggtccgc
     8161 gtccatctcc gtcggccgga cccgacggcg cccggctcct tcgttccggc ggtcggtctc
     8221 gtttcgatca atcagggcgg gccgtacgcg caccgcttcc cctcgaatgc atcgttccgg
     8281 acggacgccc agggcgagct gacgatcatc gatgtcggcg gcgggtttgg gctgtcggtc
     8341 cgcgccgtcg aggacggcac gggcgccggc ggctccgcca ccgttcctga cctcatgctc
     8401 gatggccagc aggcggaggt aacggtcgtc gtcgagccgc gaggcatcgt gcgcggtcgc
     8461 gtgcttctcc cggacggcgt cactccggcc tccggagtcc cggtccgcct caacgtcgcg
     8521 ggatggctga tctcgccctt ctccccgccg cccgcgacgg tttcggtgat cgccggcccc
     8581 gacggccggt tcgagcttcc ggacgtacct ctccagagct acttcgtcat cgccgaggcg
     8641 acggagcccc cgtcgaaggt ctacgtcgag ggcagcatca gccggagcga tctggtcgcg
     8701 gatctgggcg acctcgtcct cgacgacgcc cggccggcgc tcgtctccgt cgagcctccc
     8761 gaagggacgg cagagctcgg cctgaccccg acgatccgct tcaccttctc cgagccgctc
     8821 tggccgtacg gcgacagcgg actctggaac ctcgttcggg tcacagcacc gacgggagac
     8881 atctcgacgg ggttccgggc atcgctggac gccacgggcc agatcctcac ggtccagccg
     8941 aagtacccgt tgccgggcgc caccctctac gaggtccggg tctcggggaa cctccgagat
     9001 cgcaataccc tcgccaccgg tgtcgacgcc gtcacccggt tcacgacggc cgacctgacg
     9061 aaccccgtcg tcctccggac gaccccggtc gccgatcaga tccaggtctc cgttctcgtc
     9121 aacccatcgg tcgtcctctc gaaggcgatc gatccggcga cggtcgtcgc cggcacccgc
     9181 ctttaccggc tcgacgcggc gggctcctcc gtcgcgatct ctccggccct ccaggccgac
     9241 ggccagacca ttgtcctcaa ccccgaggcg agcctcgaag ccgaggcgga gtacgagatc
     9301 gtcgtcgacg ctctgaagga catcgcagga aacccgctcc tcgccatcac ccgggtccgc
     9361 ttcttcacgc gggacgacgc cgccccgctc ctcgccttcg atccgcccga cacgccggag
     9421 ccgatcgagg gcacgacccg ctcctacacc gtccgcttcg gcgacgatga cgtcgagacg
     9481 gccactctct acctcatcgc gccgacgggc gagatctgga atgccacaaa gagcgtcgtc
     9541 tcgccggcgc gggacgcccg ctccatcacc tggtccgtcc ggctgccgcg cgtcgccgag
     9601 gcgggcggga cggcggtccg ctgtcgcgcc ttcgcggtcg acttctgcgg caactcctct
     9661 gctcccgtcg accttccgct gacgctccag atcgactcat tgcccaccct ggtctccgcc
     9721 gaggcatcca ccgcaaccgt gcggatcggc gggaccttca cggtcgccgt ctccgcgacc
     9781 gacgacctct cgctcgccgg gatcgacgcg tccccgtccg cggggatcga agcggtctcg
     9841 gccgaggtga ccgcctcgtc tccgacgagc cggaccgagc tccgcaccta ccgggtgcgc
     9901 ctcgacgccg cgcccggggc ggcgagcgtc tcgtttgttg cccgcgactc gcgcgggcag
     9961 ctctcgcccg cgacgtccgt ctccgtgacg gttcagccgg acgacgcgcc cgtcgtctcg
    10021 atcctctcgc cggcgtccgg ctcgagcgtc ctctccggcg cgagcctgcc ggtcacctac
    10081 tccgccacgg acgacctcgg ggtcgtttcc ctcgtcgttc ggctcggcga atcgagcgtc
    10141 gacgtgccga gccccgctgc gaccggcacg gtgaacgtcc gcgtgcccgt cgtgacgacg
    10201 gtcggggctc acgacctgac cgtgaccgcc accgactcga cggggcacgc cgtttcggcc
    10261 acggtttcgt tgacagtgcg gccggacgag ccgccgtccg tcttgatcag cgcgccgccc
    10321 gcgggggcaa ctctcgtcag cggagcgggc gtcccggtca cctacacggt ctccgacgac
    10381 gttggggtcg tcacgctcct tctccgtctc ggagactcgc aggtgccgat ctcgaacccc
    10441 tcgggcacgg ggacggcgac cctcgtcgct cccgtcgtag cagccgccca gagcctttct
    10501 cttacggcca gggcgaccga cgcgtccgga cacgtcgccg aaacgagcgt cgccgttacc
    10561 gtgaagccgg acgatccgcc cgcggtcgcg attctctcgc ccgcggtcgg aacgagcgtc
    10621 ctctccggtg cgacggtccc agtcgcctac agcgtttccg acgacgtcgc tgtcgcctcg
    10681 ctcgaactga agctgggctc gtcgagcgtc atgctcgtgc gcccgcccgc ggtcgggacg
    10741 acgaacctga ccgcaccggt cgtgaccgcc gtggagccgg tcgtcctctc ggcgacggcc
    10801 acggactcgg cggggcacgt cgtcccgacg agcgtcacgc tctccgtccg tcccgacgag
    10861 ccgccgacgt tcacggtcac ggcgccgacg gaggggctgc acgtcaagag cggggcttcg
    10921 ctcctcgtga gtggatcgct cgcggacgac aacggccgcg cgacgctcac ggccaccctc
    10981 ggacccgcca gccggtccgt cgcgtccgtc ggtccgttct cgttcaccct ccccgttccc
    11041 gtcgtggccg tttcgacgcc gatcaccctc gccctgcgcg cgcaggactt cacggggcac
    11101 accgcgacgc cccaggacct cggcctcgtc gtcgagccgg acacggtctc gccgacgatc
    11161 gtcgtcagca gcccggctcc gggcagctcg attgtcggcg gcaaggcgtt cgcgctcgtc
    11221 gccacggcga gcgacgacgt cgccgtcacc ggcttccgct accgcgtcga ccagggggct
    11281 ttcgtcccgg tcgcggggcg agtgctctcg gtgcaggtcc tgaccgggac cgtctccgtt
    11341 ccgaccccga tgacgatcga ggtcgaggcc gaggatgggg cgggacacgt caccccgtcg
    11401 acggtcggcc tgaccgtcgt cccgaaccag gcacccacgc tgagcctcac ccggcccgcc
    11461 gccgaggccc ggatccccgc cggggcgacg ttcgccgtgg aagggacttc ggtggacgac
    11521 tccggtgtcc cgacgacgac ggtcaccttc gacggccaga cgaggtcggc gacctcgaac
    11581 ggcttcgccg tcacgttcac agcgccgttc gtctcgaccg agacgacgtt cacgctgtcg
    11641 gcgacggcgc gcgaccccga ggggaacgag tctcccgagg tgtcgcgctc gctgacggtc
    11701 gtccccgacg tcggcgggac gccgaccgtg cagctgacgg ccccggcgcc gggtgtcctc
    11761 ctcgtcggcg agaccgcagc gatctcgatt acctttgccg acaacgtcgg cctctccttc
    11821 ggctccgccg aggtcacggg gccgttcacg tcgggcccta ggacctggtc cctctccggc
    11881 acctcctcgg tccagagcgt cgagatcgcg ccggcggatc ggccgttcgg cgagaaggcg
    11941 cgcgtcgtgg cgaagaacgt cgacctcggc ggtcgcgtcg cgaacctcga cgtctctatt
    12001 ccggtcgcct atcaccgcct cgagctgccg ctgccctccg gggcgctcgc cgaaggcagc
    12061 accctcaccg ccgtcttccg gatcggagag tcggggcgcc agcgcgcctc cgcgctccgc
    12121 ctcgagatcg gcacggtctc ggggcagacc ttcacggtcc tcgcgtgcgt ccagacggcg
    12181 gctccgctct ccgagcttga gacgctggcc gtccgcgttc ctgtcggacg gaccgggctc
    12241 ttcgtccggt cgatcctcgt cgagacgacg ggcgacctgg cacaggccgc ggccttcgac
    12301 gggacagcgg tcctcctcga gcctctctcg acctcgggcg acacggcggc gccgaccgtc
    12361 gtcatcatga atcccgcaga cgggctctcc gttctgagcg gcgacctcgt ccagacgagc
    12421 gtgtcggccg ctgacgacac gggcgtcgag cggatcgacg cctcgttcgg cggcatcacg
    12481 aagacgtgct cggcggccac ctgcgagctc acgttcttcg ctccggtcgt cgcgacggcc
    12541 gggacgcaga ctctctcggc gacggcacgc gacggggcgg ggaaatcgac tacgacgagc
    12601 gtggccgtga ccgtctcgcc gcgctcctca gggacaacat ccggtccgga gccgggcgag
    12661 ctcgtcgctg gcgatggaag ggcccccatc gtccggttcc tgacgcccca cgtctctccg
    12721 tacccggtcg ctcccaatgc tccgttccgg ccggcagtcg aagcggttga cgcggacgga
    12781 attgaccgga ttgagctctt cctcggcgag accgacgtcc cctgcctcgt ccttcgtccg
    12841 gacctgccga gtcgggacgc atgcttcgtt cccgacgcgg ccgccggctc cacgctctgg
    12901 cttcgcgccg tcgcgttcga cctgtcgggc gaccggagcg aggtcgaggt agagctcgtc
    12961 gtcaccccgg gcatccgaat acccgaggac gacgtttcgc ctgcggaacc cgatcgtccc
    13021 gaggagacag agtagatgga gcctcagacg ctcctcgatc gttcgctgga ggccagcgca
    13081 tccgtcgtgt tcggcggcga cggagccgtt gccgcgtcgg aggctcctct cgtcctgctg
    13141 gcgacccggg acccgttcct cgacagcggc ggggtcggct ccgccggaac tttcctcgtc
    13201 cacggcgccg cgcatcccca ctcgagcctg gagcagccgt gaaacccact cgtctcgtcg
    13261 tcctcgccct cctcggactc ctcgtcgccg tccccccggc ggcggcgcag caggatccct
    13321 tcgcgatccc gcaggccctc tccggccagg cggtcttctc cgcccaggcg ctcacggtca
    13381 acggcggcgc cgtcgtcgac tcgagcggcg tcgccggcgg ctccggcgcc ggctcggagg
    13441 ggcacgtcgt ctcgaactcc gacatcaccc tcaacggcaa gtcgaaggtg aaggggagcg
    13501 cctggtacgg accggggaag aagatcgtcc tgaacggagg ctccacggtc tccggcacga
    13561 cggcggcgct cgccgcggcg cgggacgcgg cgccgatcgc gctttcaggc ctcgcggcga
    13621 cgctcgcggc ctcgaacgac aacgccctca tcgggaagac gagccggaac cggagcccgc
    13681 tccagggctc gggtgggacg gacttcgtcc tgaacggaaa cgagacgatc acgatcccgg
    13741 cggggacctt ctacttcacg acgatcacga tcaacggctc ctcgaagctg agcttctccg
    13801 gcctcacgcg gatcctctgc accgggcccg tgacggtgaa cggcacctcg acggttcacc
    13861 agaacggcaa tccctggctc ctgcggctct gggtctccgg ggcgcaggtc acgctcaacg
    13921 gcaacagcac cctgaacggc ttcgtctact ccccgtcggc cgcggcgacc gtcgacggcg
    13981 gcgggatcct cacgggcggc ctcttcgccc agaaggtcac cgtcaacggg ggagggcgcg
    14041 tcacgcgttc cttcgacgac acgtccgccc tcgcggcgac cgtgacgaac gcgggccagc
    14101 cgatcgcgaa cggcgccgtc ttccgcgccg aggtcgcgcc cgtcgtcacc gtcacgggcg
    14161 gtgtcccgcc gctcgcgacg cgggtcacgc tcgacggcgc cccgttcgtg tcgggcacca
    14221 ccgtctccgc cgaaggcgtc caccgcctcg tcgtcgtctc ggacgacctc gcgggccggc
    14281 acctggagct gaccgtcggc ttcgtcatcg acaggtcggg gccgtcgctc gcgatcgtcc
    14341 gccccgcgaa cggcgaggtc gtcgggacgg ctccggtcga cgtgaccggg acctctggcg
    14401 acgcgacctc cgtgtcggtg aacggtgtcg ccggcaccgt cgccggcggc tccttcgtcg
    14461 ttcgcggtgt tccgctcgcc gaagggacga acacgctcgt cgccgccggc gccgacgccg
    14521 ccgggaacgc gggctccgcg acctccgtcg tcgtcctcga cacgggggcc ccggtcctta
    14581 ccgtcgcctc tccggcggac gggctcctga ccaaggccga gacggccctc ctccagggcg
    14641 ccgtctccga cccgcacctc acctccctca cggtgaacgg gctgccgatt tccgttccgt
    14701 cgggcggcgg cgccttctcg cgggaggtcg cgctcgtcga gggcgcgaac gcgctgacgg
    14761 tggaggcgcg cgacgtcgtg gggcacatcg cgagccgctc cctcacagtc cggagggacg
    14821 gcgtggcgcc cgagatccgg atcgaggagg ggaacctccc cctcgaggcc gggcgcgcgt
    14881 acaacaggga cctcacgatt cgcgtcaccg tcgtcgaggc gaaccccggc accctggagg
    14941 tgacgctcga cggcgcgacc ttcgtgtccg gggcgaccgt ctcggccgaa ggggcgcaca
    15001 cgctcagcgc gacggctgtc gacggcgcgg gcaacagcgc ctcgaagacc gtctcgttct
    15061 cgatcgacaa gacggctccg tcgctctcga tcacgacgcc gacaccgggc tcgacgatcc
    15121 tcgacctccc ccggacgctc gcagggacgt ccggcgacgc ggtcacggtc gacgtcgacg
    15181 gcaccgtcgt cgcgccggcg ggaggggcct tctcgatccc ggcctttccg tttgccgagg
    15241 gacccgtcac tgtcacggcg acgggcaccg acgccgccgg gaactccgga acggcgacgg
    15301 ccacctacct cgtcgacacg ctgccgcccc aggtgacgat cgcgagcccg ctcgaaggtg
    15361 tgcggctcgg agcggccccg atcttcgtaa cgggtgtcgc ctccgacgcg aacctcctcg
    15421 aggtccgggt caacggccag gcggcgagcc tgggctccga cggcagcttc caggccgggc
    15481 cgttccgtcc cgcggacggc
//
LOCUS       DAOTEZ010000049        15360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2634_length_15360_cov_5.989023, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000049 DAOTEZ010000000
VERSION     DAOTEZ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15360
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2634_length_15360_cov_5.989023"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1553)
                     /locus_tag="P5164_04045"
     CDS             complement(<1..1553)
                     /locus_tag="P5164_04045"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HRY43099.1"
                     /translation="MSGDRLLAIDHGTQSVRALLFDLSGNLVAGSRVPIEPYVPGPPG
                     FAEQDPELYWRTVCAATRGLLAQPGVSPGSIAGLAVTTQRATMVATDAKGRPLRPAIV
                     WLDQRRTEGVPPVRGLWGAAFRLSGMSGTVGYFQAEAEAHWLARHEPETWRKTERYLF
                     LSGFLAHRLTGRFVDSTGGQVGYVPFDYRRRRWAGRLDWKWEALPVRREQLPELVPPG
                     GRLGELTAAAAEATGLPKGLPVLAAAADKACEVLGAGCLEPHVACLSYGTTATINTTH
                     RRYVEPLPLLPPYPSAVPGAYSLEIQIYRGFWMVTWFKEQFGQLEEAEAREKGLPAEA
                     LFDELVSKVPPGSMGLVLQPYWSPGVKRPGPEAKGAVIGFGDVHTRAHLYRAILEGLA
                     YALREGKERSEKRSGVPITQLRVAGGGSQSDAAMQITADVFGLPAARPHVPEASGLGA
                     AIDAAVGLGLHPTFEVAVASMTRVGRVFIPDPKTSSLYDSLYRRVYRRMYERLRPLYA
                     EIREITGYPP"
     gene            complement(1550..3133)
                     /locus_tag="P5164_04050"
     CDS             complement(1550..3133)
                     /locus_tag="P5164_04050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010396623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HRY43100.1"
                     /translation="MTRAADRLRLPESADLLVVGGGITGAGIALEAARAGASVVLVEQ
                     GDFASGTSSRSSKLVHGGLRYLAEGNLRLTREAVRERDRLLDEAPGLVDPVGFLFTLR
                     DGEKPGAALVGTGLALYDLLARQGRHARLTPDELLLRAPHVGGPGLSGAFAYADAATD
                     DARLVLRVLHEAAAAGAATRNYVRCEGLLRRGGRVAGALLRDVRTGEEHPLGAAAVVN
                     ATGAWADRLRAEVGGRPRIRPLRGSHLLFPQWRFPTAQAVTFRHPLDARPLFAYPWEG
                     LTLLGTTDLDHPGTLDEEPSISAPEVDYLLQGARRAFPSLALSARDAVSSFAGVRPVV
                     GTGKARPSEESREHVLWEEEGLVTVTGGKLTTFRLIAVETLRLLRRRIPAFERVRADA
                     GVFRPSGAPPQEAARLGAATARRLAGRHGAAAAEVVAGARPGELEPVAGTPWLWAEIR
                     HAARAERVVRLDDLLLRRVRVGLLLPDGGASLLPRVAAICREELAWDDERWRMEEQDY
                     LARWRASYSAPAAPAALCA"
     gene            complement(3130..4722)
                     /locus_tag="P5164_04055"
     CDS             complement(3130..4722)
                     /locus_tag="P5164_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRY43101.1"
                     /translation="MRRWNGWGDDTVSSSIPPRAAEALAAWVGPGAPPRDAAPEAALA
                     AVPPSRLAAEGGLSVDPWDRLLHARGQSLPDWVALRSGRLGVVPDAVACPESGEQVAA
                     LVARAGREGLLLVPYGGGTSVAGHVTPVASDRPVVSVDLSRLSRLVSLDESSRLATFG
                     AGVKGPDLEAELRARGLTLGHFPQSFELSTLGGWVATRSSGQQSLGYGRIERLFAGGR
                     VETPSGRLDLPAFPASAAGPDLRELVLGSEGRLGVISEATVRLSPLPAREEVHGVFLP
                     DLASAVAAVRDIAAMRLPLSMLRLSTPEETRTTLLLAGHARLIRALETYLSFRGAREE
                     KCLLLVGVSGGENLVRQTRRQVLGALAMRGGVAAGRAFGREWHRNRFRAPYLRNALWE
                     AGWAVDTLETAAPWSRVPALLASVERALRVGLESEGERVHAFTHLSHVYLDGSSLYTT
                     YLFRIDRDPDVTLARWRALKTAASEAIVAGGGTISHQHGVGTDHAPWLAAEKGALGVR
                     ALGDVLRSFDPQGLMNPGKLLP"
     gene            complement(4719..5492)
                     /gene="fadR"
                     /locus_tag="P5164_04060"
     CDS             complement(4719..5492)
                     /gene="fadR"
                     /locus_tag="P5164_04060"
                     /inference="COORDINATES: protein motif:HMM:NF019456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HRY43102.1"
                     /translation="MVRPLTSRPVSPARVGESAASGVAPSPRRPGAHAERELAAALLS
                     GRFAPGSSLPPERELAAALGVTRPTLREALKKLDRDGFVSVRHGLPTRVNDVWTEGGL
                     NVLAALAEHGEVPKGFVKQLLEVREVLAPAYAREAVRRAPARVATYLAASGALGNDAE
                     AWTRYDWGLHRLLAISSGNPVYVLILNGFRELFARMALLYFERPAARAASREFYAALH
                     AAAEAGDPDAAGAAARDVMARSVDLFRPIEARLAGRRAR"
     gene            5642..6133
                     /locus_tag="P5164_04065"
     CDS             5642..6133
                     /locus_tag="P5164_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43103.1"
                     /translation="MTSPSPLVDLLKRHETAILAETFDGMTRCRLTRYQAAGDEETRR
                     RLSRLFALLLDAVNERDLEPVLAYAREIARQRFGEGVGLQEVQTAINILEEAVWRRVV
                     KEVPPDGLAEALGLVSTVLGVCKDTLASSYVGLATRRHAGSLDLTALFRGTQNRFYRG
                     TEE"
     gene            complement(6156..6920)
                     /locus_tag="P5164_04070"
     CDS             complement(6156..6920)
                     /locus_tag="P5164_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HRY43104.1"
                     /translation="MRRPVSLSLASAALALLVLSACGAGREEGSAAARDLVPTNPGAA
                     PRDATDGRSPGPGDAPAPEGAVPADAPLVVFLGDSLTAGLGLPVDQAYPAVVAAELAR
                     KGKPVRVVNAGVSGDTTAGGLRRAEWVLTQRPDVLVLALGANDGLRGLPLADTERNLR
                     GIVSKARDAGAKVLLCGMLVPTSHGPDYQRGFGALFPRVAKETGIPLVPFLLEGVAGR
                     PERNLEDGIHPNAEGQKVLAANVLPLLEPLLPSGAR"
     gene            6957..7643
                     /locus_tag="P5164_04075"
     CDS             6957..7643
                     /locus_tag="P5164_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY43105.1"
                     /translation="MRTPLLELRGVSKELPSGGRTLRILDAIDLSIAAGEFAAILGPS
                     GSGKSTLLGLMAGLDRPTRGEVLLEGEAIHRMTEDELALLRRRKVGFVFQSYQLLSNL
                     TARENVLLPLELTGQAGAGARATELLAAVGLGERGHHYPSQLSGGEQQRVALARAFGT
                     RPPLLLADEPTGNLDSATGASILDALVRLRSESGTTLVLVTHDPAVAALADRRVHLRD
                     GRIERDETAS"
     gene            7640..10189
                     /locus_tag="P5164_04080"
     CDS             7640..10189
                     /locus_tag="P5164_04080"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY43106.1"
                     /translation="MNGGLFFRQLVRESRGSRGRLAFFAACLGTGVAAVVAVAGLSGA
                     LEESVRSQARPLLGADLAVESFQPFPAAAESAVAALPGTRTRSEELVTVLYEPGAAAA
                     VEGAPPRSLLVELKAVEAPYPLAGELRLEPDRPLADLLPPDGLVAHPDVLRRLGVAVG
                     GDVSLGGAPFRVTGTIASEADRLAGSFRLGPRVFVSRAGLARTSLAGFGSRVTRRILV
                     NLPEGTDVPAARRRVSALREQLDGVPGVRVESWLDGQPELRQAIRRTSRFLGLVALAS
                     LLVGGVGVAQTVRAWIASRLDALAILRCLGVTSRETVLLYGVQTAGLGLVGSLLGAVA
                     GIGLVAAVGHTYSDLLPPGGLDPWQPAALLRGLGLGVGVSVLFGLPALLAVRRVPPVR
                     VFRHDAEPLPASRGLRLAAAALLLAGVGLTAWVQSGLPRVAVSFTLGLAASALLLFLA
                     AHALLRLVARVPRERLPFALKSGLSALSRPGAGTVGSILALGLGTLVVLQIALVQRDF
                     SRQLTADLPRNAPTAFLLDVQPDQWPGVAALLSANGATRTLSVPVVTARLTAVDGTPT
                     AELAKRRAGDSRRERWALTREQRLTYMTELPEDNRVVAGALWSDPSAAEVSLEEEFAG
                     RIGAKVGTWLDFDVQGVPLRLKVTSLRSVDWRTFGINFFLVVEPGVLEKAPQLRLAAA
                     RLPSAHEGRIQDLLAREFPNVVLIRTREVLERVAAIATRLGKAVRVLGAFSVLAGLVI
                     LAGSISAASARRAREAALLKVLGVTRGGVATALAVEYALVGLVAGLVGTLGATLLAGS
                     VLTAAFEVSFRPSPLLLAATPLAVALLAAAAGLAVSLRALQVRPLEVLRGE"
     gene            complement(10232..11572)
                     /locus_tag="P5164_04085"
     CDS             complement(10232..11572)
                     /locus_tag="P5164_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43107.1"
                     /translation="MDALDQAVATAVLGLDTLWGGDVMNPSGTGRFIADSWFSDEPLP
                     ASYTRPEAARLRELGGASAKPETVAALDAYLDAVDVRGAIARVRDEAGRGAGLRARYL
                     EGLGLCFEVMLDLALEVAGRGEPVPYERCMRAILGRPGSPSEPAAKRERVRELLSAAG
                     HPSTDGPGLLAAVDAWRAERIVPKEEIPALSARLVAELDTLAARHVMPFLPAEVARVP
                     RANVTFLPIENAWFSGSMNYLGRARRPDGSPEYEATYELNASLQISRPEFAQLVSHEV
                     VPGHVTTFAFLQDLFVRGIVGFEASVLTMNTRPAALFEGIANNALLMAYGVFSPEELP
                     DREMEAGMLLALLQDDAKNQSSWLTWNEGWAKEDVARVLRNDYLVSEERADKLSGAWG
                     RHPLLGRMYLPAYRAGTEKVAELRKLHPAAKVLPALYGALGVVDLTTVEDAIAD"
     gene            complement(11576..13630)
                     /locus_tag="P5164_04090"
     CDS             complement(11576..13630)
                     /locus_tag="P5164_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014562003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HRY43108.1"
                     /translation="MMEVSSSKSLPENAYRPLKPGETYQPIVPADAAIQEFTPRSVTL
                     GIVMAAVFSAAAAFLGLKVGQVFEAAIPIAILAVGLGYAFKRRSTVLENVIVQSVGAA
                     SGLVVAGAIFTLPALFILGLPVDLFKLFLVSLLGGALGIVFLVPLRRHFVKEMHGEFP
                     FPEATATTEVLVAGEAGGKQAKVLVAAALIGGIFDFLILHLQAFAEVFTTRSVWLLDK
                     VAERSKIVFKIDVLSSVMGLGYIIGLKYSAVICAGSFLSWFLLVPVIAHFGAGATTGI
                     PPVTDGTLVGAMGAEAVFRNYVRLIGIGAIAAAGILGILRSWRVIAKAFSFKAIFGGA
                     KSASGETERTDRDLPMKVILGGLVAVALVAWAFFRFGVLDPSPSIGTQSLIALLVVIV
                     ISFLFTSVAARAIATVGTNPVSGMTLMTLILTSLFLVKAGLSGNAGMLAALLIGGVVC
                     TALSMAGGLITDLKVGYWIGATPAIQQRSKFLGTIVAALSVGSVLLLLNKVYGFVPGP
                     DHPAGQVLAAPQANAMAAVIKTLMSAEPVPWLLYGTGALIAISMQMIGVPALAFALGM
                     YIPLELNTPLLVGGFIAHLVAKSAGKDEKLAAARNSRGILIASGFIAGGAVMGVVATV
                     FKFLATDEKVAGAIGTGIPSLLGWNFGFSEGMGPELLALGAYVLLAAYMYWDARRTNG
                     EE"
     gene            complement(13793..14719)
                     /locus_tag="P5164_04095"
     CDS             complement(13793..14719)
                     /locus_tag="P5164_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43109.1"
                     /translation="MTPSESEAFVPCNPDLVREIRGLAVAAHERFEAEVWARSGEVVL
                     PGVVRPPSYHNARHVAAVCDCVEALFGALGEGLDPFGLERDARRWSVEAGEPEPDAGT
                     LRTAFGFAFACHDLGNISASERVALTGSGLDLDLAKSYDSSALYGTPAVELRGAAVAR
                     ELLLALGGACGRVPALARLVEHLVLQTVFHFEKVSDEAPFWLPMQAVDMIGSYFFLST
                     SRLEAIAGLFAEMKAQRPGRIAVLPFLSSLETRFERLVPSPLLRRQVLEIFERNPYGR
                     TREAVFGVPGRFLGMVEPLPYEEAIAALLAGD"
     gene            14818..>15360
                     /locus_tag="P5164_04100"
     CDS             14818..>15360
                     /locus_tag="P5164_04100"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HRY43110.1"
                     /translation="MNPDLAYEALPRLLVAALGGLAVGVEREWSARAAGGVRRFAGVR
                     TFLLIGLSAGTAAAVSGSGAAAAGLLLLGSIALLVVASFTVHAFAGDLDATTEVAALA
                     VLGAGWLSGSGRIALGSALAALVALVLAEKTKIHGLVEKLRSEEVGAGAHFAVLALVV
                     LPILPAGPFGPSPGIRPQELW"
BASE COUNT         2074 a         5732 c         5665 g         1889 t
ORIGIN      
        1 ggcgggtacc cggtgatctc gcggatctcg gcgtagagcg gccgcagccg ctcgtacatc
       61 cgtcggtaga cgcggcggta gaggctgtcg tagagggacg aagtcttcgg gtcggggatg
      121 aagacgcggc cgacccgcgt catcgaggcg accgcgacct cgaacgtcgg gtggagcccg
      181 aggccgacgg cggcgtcgat cgccgcgccg aggcccgagg cctcgggaac gtgcggacgc
      241 gcggcgggaa ggccgaagac gtcggcggtg atctgcatcg ccgcgtcgct ctgggagccg
      301 ccgcccgcga cgcgcagctg ggtgatcggg acgccgctcc gcttctccga gcgctccttc
      361 ccctcccgca gcgcgtaggc gagcccctcc aggatcgccc ggtagaggtg ggcgcgggtg
      421 tggacgtcgc cgaagccgat gacggcgccc ttggcctcgg gcccgggccg cttcacgccc
      481 ggcgaccagt agggctggag gacgaggccc atcgagccgg gggggacctt cgagaccagc
      541 tcgtcgaaga gggcctcggc ggggagcccc ttctcgcgcg cctccgcctc ctcgagctgg
      601 ccgaactgct ccttgaacca ggtcaccatc cagaagccgc ggtagatctg gatctcgagg
      661 ctgtaggcgc cgggaacggc cgacggatac ggcggcagga gcgggagcgg ctcgacgtag
      721 cgcctatggg tcgtgttgat cgtcgcggtc gtcccgtagc tgaggcaggc gacgtgcggc
      781 tcgaggcagc cggcgcccag cacctcgcag gccttgtcgg cggcggcggc gaggaccggg
      841 aggcccttcg gcaggccggt cgcctccgcg gcggccgccg tcagctcgcc gagccggccg
      901 cccggcggga cgagctcggg gagctgctcg cgccggaccg gaagcgcctc ccacttccag
      961 tcgaggcggc cggcccagcg acgccgccgg tagtcgaacg ggacgtaccc gacctgaccg
     1021 cccgtggagt cgacgaagcg ccccgtcagc cggtgcgcga gaaagccgga gaggaaaagg
     1081 tatctctccg tcttccgcca cgtctccggc tcgtgccgcg cgagccagtg ggcctcggcc
     1141 tccgcctgga agtagccgac cgtcccggac atgccggaaa gccggaacgc ggcgccccag
     1201 aggccccgga ccggcgggac accctcggtc ctccgctggt cgagccagac gatcgccggc
     1261 cggagcggcc ggcctttcgc gtccgtcgcc accatcgtcg cccgctgcgt cgtcacggcg
     1321 aggccggcga tcgagccggg cgagacgccc ggctgcgcga ggaggccgcg cgtcgcggcg
     1381 cagaccgtcc gccagtagag ctccgggtcc tgctcggcga agccgggcgg acccgggacg
     1441 tacggctcga tcgggacacg gctccccgcg acgaggtttc ccgagaggtc gaacaggagc
     1501 gcccggacgc tctgcgtccc gtggtcgatc gcgaggagcc ggtcgccgct cacgcgcaga
     1561 gcgcggccgg cgccgcgggc gccgagtagc tcgcgcgcca cctcgcgagg tagtcctgct
     1621 cttccatccg ccagcgctca tcatcccacg cgagctcctc ccggcagatc gcggcgaccc
     1681 gcggcaggag cgacgcccct ccgtccggca ggaggagccc gacccgcacg cgccggagga
     1741 gaaggtcgtc gaggcggacg acccgctccg cgcgcgcggc atgccggatc tcggcccaga
     1801 gccacggggt ccccgcgacc ggctcgagct cgccgggccg ggcgccggcg acgacctcgg
     1861 ccgccgcggc gccgtgacgg cccgcgaggc ggcgcgcggt cgcggcgccg aggcgcgcgg
     1921 cctcctgcgg aggcgccccc gacgggcgaa agacgcccgc gtcggcgcgg acgcgctcga
     1981 aggccggaat cctcctgcgc aacagacgaa gcgtctcgac ggcgatgagg cggaacgtcg
     2041 tcagcttccc gccggttacc gtgacgagcc cctcctcctc ccagaggaca tgctcgcggc
     2101 tctcctcgga gggacgcgcc ttccccgtcc cgacgaccgg ccgcacgccc gcgaagctcg
     2161 acaccgcgtc tcgcgcggag agcgcgaggg aggggaacgc gcgccgtgct ccctggagga
     2221 ggtaatcgac ctcgggagcc gagatggacg gctcctcgtc gagcgtcccc gggtggtcga
     2281 gatcggtcgt cccgaggagg gtgaggcctt cccacggata ggcgaagagc ggtcgcgcgt
     2341 cgagcgggtg gcggaacgtc acggcctgcg ccgtcgggaa gcgccactgc gggaagagga
     2401 ggtggcttcc ccggagggga cggatccgcg gccggccgcc cacctcggcg cggaggcggt
     2461 cggcccaggc gccggtggcg ttcacgacgg ccgcagcgcc gagcgggtgc tcctcgccgg
     2521 tgcgaacgtc ccggaggagc gcccccgcga cgcggccgcc gcggcgcagg agcccctcgc
     2581 agcgcacgta gttcctcgtc gcggcccccg cggcggccgc ctcgtggagg acccggagaa
     2641 cgaggcgcgc gtcgtccgtg gcggcgtcgg cgtacgcgaa ggcgccggag agccccggcc
     2701 cgccgacgtg gggcgcgcgg aggaggagct cgtcgggcgt cagtcgcgcg tgccgcccct
     2761 ggcgggcgag aaggtcgtaa agggcgagac ccgtcccgac gagcgccgca cccggcttct
     2821 ccccgtcgcg gagggtgaag aggaagccga ccgggtcgac gaggccgggg gcttcgtcga
     2881 ggaggcggtc gcgctcgcgg accgcctcgc gcgtcaggcg gagattcccc tccgccaggt
     2941 accggagtcc tccgtgaacg agcttcgagg agcggctcga cgtgcccgag gcgaagtctc
     3001 cctgctcgac gaggacgacc gaggcgccgg cgcgcgcggc ctcgagcgcg atcccggccc
     3061 ccgtgatccc gccgccgacg acgaggaggt cggcggattc cgggagccgg aggcgatcgg
     3121 cggcgcgcgt cacggcagga gcttcccggg gttcatcagc ccctgcgggt cgaacgagcg
     3181 gaggacgtct ccgagagcgc ggacgccgag cgcccccttc tcggccgcga gccacggggc
     3241 gtggtccgtc ccgacgccgt gctggtggct gatcgtcccg ccccccgcga cgatcgcctc
     3301 ggaggcggcg gtcttcagcg cccgccagcg cgcgagcgtc acgtcggggt cgcggtcgat
     3361 ccggaagagg tacgtcgtgt agaggctcga gccgtcgagg tagacgtgcg agaggtgcgt
     3421 gaaggcgtgg acgcgctccc cctcgctctc gagcccgacc cgcagcgccc tctccacgga
     3481 ggcgaggaga gccgggacgc ggctccaggg cgcggccgtc tcgagcgtgt cgacggccca
     3541 gcccgcctcc cagagcgcgt tgcgcaggta cggggcccgg aagcggttgc ggtgccactc
     3601 ccggccgaac gcccggcccg ccgcgacgcc gccccgcatg gcgagcgcgc cgagcacctg
     3661 ccggcgcgtc tgccggacga ggttctctcc tcccgagact ccgacgagca gaaggcactt
     3721 ctcctcccga gccccgcgga aggagaggta ggtctcgagc gcgcggatca ggcgcgcgtg
     3781 ccccgcgagg aggagtgtcg tccgcgtctc ctcgggagtc gacagccgga gcatcgagag
     3841 cgggaggcgc atcgcggcga tgtcgcggac cgcggcgacg gccgacgcga ggtcggggag
     3901 gaagactccg tggacctctt cccgggccgg aagcggggag aggcggacgg tcgcctcgga
     3961 aatcacgccg agccggcctt cgctgcccag gacgagctcg cgcaggtcgg gcccggcagc
     4021 ggaggccggg aacgccggaa ggtcgaggcg ccccgacggc gtctcgacgc gccccccggc
     4081 gaagagcctc tcgatccggc cgtagccgag cgactgctga ccgctggaac gcgttgcgac
     4141 ccagccgccg agcgtcgaga gctcgaacga ctgcgggaag tggccgagcg tgaggccgcg
     4201 cgcgcgcagc tccgcctcga ggtcgggccc cttcacgccg gcgccgaacg tcgcgaggcg
     4261 gctcgactcg tcgagcgaga cgaggcgcga gaggcgcgac aggtcgaccg agacaaccgg
     4321 ccggtccgac gcgacggggg tgacgtgtcc cgccacgctc gtcccgccgc cgtacggaac
     4381 gaggaggagg ccttcccggc ccgcccgcgc gacgagcgcc gcgacctgct cgccgctctc
     4441 cgggcaggcg acggcgtccg gcacgacgcc gagccgcccg gaacggagcg cgacccagtc
     4501 gggaaggctc tgcccccgcg cgtgaaggag ccgatcccac ggatcgacgg agaggccgcc
     4561 ttccgcggcg agccgcgacg gcggcaccgc cgcgagcgcc gcctccggcg ccgcatcgcg
     4621 cggcggcgct ccgggcccga cccacgccgc gagcgcctcg gccgcccggg gcggaatgga
     4681 ggaggaaacc gtgtcgtcgc cccagccgtt ccagcgcctc atcgcgccct cctgcccgcg
     4741 agccgggcct cgatcggccg gaagaggtcg acgctcctcg ccatcacgtc cctcgcggcc
     4801 gctcccgcgg cgtccgggtc ccccgcttcc gccgccgcgt gcagggcggc gtagaactcc
     4861 cgcgaggcgg cgcgggccgc cggacgctcg aagtagagga gcgccatccg ggcgaagagc
     4921 tcgcggaagc cgttgaggat caggacgtag accgggtttc cggaggagat cgcgaggagg
     4981 cggtggagcc cccagtcgta gcgcgtccag gcctcggcgt cgttcccgag cgccccggac
     5041 gcggcgaggt acgtggcgac gcgggccgga gcgcgccgca ccgcctcccg agcgtaggcc
     5101 ggcgccagca cctcgcgcac ctcgaggagc tgcttcacga agcccttcgg cacctcgccg
     5161 tgctcggcga gcgccgcgag gacgttcagc cccccctcgg tccagacgtc gttcacgcgc
     5221 gtcggcagcc cgtgccgcac ggagacgaag ccgtcgcggt cgagcttctt gagcgcctcg
     5281 cggagcgtcg ggcgcgtcac gccgagcgcc gcggcgagct cgcgctcggg agggagcgac
     5341 gagcccgggg cgaagcgccc ggaaaggagc gccgccgcga gctcgcgctc ggcgtgcgcg
     5401 cccgggcgcc ggggcgacgg ggcgacgccg gaggcggcgg attcgccgac acgggcggga
     5461 gagacgggcc tactggtaag aggtctgacc acatggaaag tctgggaccg cccgggccgt
     5521 ccgtcaaggg aggactccgc ccgggccgga cgaccgcggc ccgcgcggcc ggcccgcacc
     5581 cgtcccgacc ggcctccccg tatgattcgg agagcgccgc gaacgccccg gaaggagccg
     5641 catgacgtcc ccatcgcccc tcgtcgacct cctgaagcgc cacgagaccg ccatcctcgc
     5701 ggagaccttc gacgggatga cccgctgccg cctgacgcgc taccaggcgg cgggcgacga
     5761 ggagacgcgg cgccgcctct cgaggctctt cgccctcctc ctcgacgcgg tgaacgagcg
     5821 ggacctcgag cccgtcctcg cctacgcgcg ggagatcgcg cggcagcggt tcggcgaggg
     5881 ggtggggctc caggaggttc agacggcgat caacatcctc gaggaggccg tctggcggcg
     5941 cgtcgtgaag gaggtccccc ccgacggcct cgccgaggcg ctcgggctcg tcagcaccgt
     6001 cctcggcgtc tgcaaggaca cgctcgcctc ctcgtacgtc ggcctcgcga cgcgccggca
     6061 cgccgggagc ctcgacctga cggccctctt ccgggggacg cagaaccgct tctaccgcgg
     6121 gacggaggag tagctcctcc gggaagcccc gctcctcagc gcgcgccgga cggcaggaga
     6181 ggctcgagga gcgggaggac gttcgccgcg aggaccttct gcccctccgc gttcgggtgg
     6241 atgccgtcct cgaggttccg ctccggccgt cccgccacgc cctccaggag gaacgggacg
     6301 agcgggatgc ccgtctcctt cgcgacgcgc gggaagaggg cgccgaagcc ccgctggtaa
     6361 tcgggaccgt ggctcgtcgg gacgagcatc ccgcagagga ggaccttcgc gccggcgtct
     6421 cgggccttcg agacgatccc gcggaggttc ctctccgtat ccgcgagcgg gaggccgcgc
     6481 agcccgtcgt tcgcgccgag agccaggacg aggacgtcgg ggcgctgcgt gaggacccac
     6541 tccgcgcggc gcaggccccc cgcggtcgtg tcgcccgaga ccccggcgtt cacgacgcgg
     6601 accggctttc ccttccgcgc cagctctgcg gccacgacgg ccgggtaggc ctgatcgacc
     6661 ggtaggccga ggcccgccgt caggctgtcg ccgaggaaga cgacgagcgg agcatccgcc
     6721 ggcacggccc cctccggcgc aggagcatct cccggccctg gcgagcgacc gtcggtcgcg
     6781 tcgcggggcg ccgccccggg gttcgtcggg acgaggtcgc gcgccgcggc ggagccctcc
     6841 tcccggcccg ctccgcaggc cgagagaacg agcagcgcga gcgccgcaga ggccagggag
     6901 agcgaaaccg gacgcctcac cgcgacggag tctaagggaa tgcgagactc cgtcccgtga
     6961 gaactcccct tctcgagctt cgcggcgtct cgaaggagct tccgtcgggc gggcggacgc
     7021 tccgaatcct cgacgccatc gacctctcga tcgccgccgg cgagttcgcc gccatcctcg
     7081 gcccctccgg cagcggcaag tcgaccctcc tcggcctcat ggccggcctg gatcgtccga
     7141 cgcgcggaga ggtcctcctc gagggagagg cgatccaccg gatgaccgag gacgagctgg
     7201 ccctcctccg gcggaggaag gtcggcttcg tattccagtc gtaccagctc ctgtcgaacc
     7261 tgaccgcgcg ggagaacgtc ctcctcccgc tcgagctgac cgggcaggcc ggagccggcg
     7321 cacgggcgac ggagctcctc gccgccgtcg gactcggcga gcgcggccac cactatccct
     7381 cccagctctc gggcggcgag cagcagaggg tcgcgctggc ccgcgcgttc gggacgcgcc
     7441 ccccgctcct cctcgccgac gagccgacgg ggaacctcga cagcgcgacg ggcgcctcga
     7501 tcctcgacgc cctcgtgagg ctccggagcg agagcgggac gacgctcgtc ctcgtcaccc
     7561 acgaccccgc cgtcgccgcg ctcgcggacc ggcgcgtcca cctccgcgac gggaggatcg
     7621 agcgggacga gacggcctcg tgaacggagg cctcttcttc cgccagctcg ttcgcgagag
     7681 ccgcggctcg cgcgggcgcc tcgcgttctt cgccgcctgc ctcggcaccg gcgtcgcggc
     7741 cgtcgtcgcc gtggccggcc tctccggcgc gctcgaggag agcgtccgaa gccaggcgcg
     7801 cccgctcctc ggcgccgacc tcgccgtcga gtcgttccag cccttcccgg ccgccgccga
     7861 gagcgcggtc gccgccctcc ccggcacgcg gacgcggagc gaggagctcg tgaccgtcct
     7921 gtacgagccg ggcgctgcgg cggcggtcga gggagcgccg ccgcgcagcc tcctcgtcga
     7981 gctgaaggcg gtcgaggcgc cctacccgct cgcgggtgag cttcgcctcg agcccgaccg
     8041 gcccctcgcc gacctcctcc cgcccgacgg cctcgtcgcc caccccgacg tcctccgccg
     8101 tctcggcgtc gccgtcggcg gggacgtctc gctcggcggc gccccgttcc gggtgaccgg
     8161 gacgatcgcg tcggaggccg accgcctcgc gggctccttc cggctcggcc cccgcgtctt
     8221 cgtctcgcgc gccggcctcg cacggacctc gctcgccggc ttcggcagcc gcgtcacgag
     8281 gcgcatcctc gtgaacctcc ccgaggggac cgacgtgccg gccgcccgcc gccgcgtctc
     8341 ggcgctgcgg gagcagctcg acggcgtccc gggcgtccgg gtcgagagct ggctcgacgg
     8401 ccagcccgag ctccggcagg cgatccgacg cacctcacgc ttcctcggcc tcgtcgcgct
     8461 cgcctcgctc ctcgtcggcg gggtcggcgt cgcccagacc gtccgcgcct ggatcgcgag
     8521 ccgcctcgac gccctcgcga tcctccgctg cctcggcgtc acgtcgcgcg agacggtcct
     8581 cctctacggg gtccagacgg caggcctcgg cctcgtcggg agcctcctcg gcgccgtcgc
     8641 ggggatcggg ctcgtcgcgg ccgtcggcca cacctactcc gacctccttc cgcccggcgg
     8701 cctcgacccg tggcagccgg cggccctcct ccggggcctc ggcctcggcg tcggggtgtc
     8761 ggtgctcttc ggcctcccgg ccctcctggc ggtccggcgc gtcccgcctg tgcgcgtctt
     8821 ccggcacgac gccgagccgc tcccggcgtc gcggggcctc cggctggccg ccgccgcgct
     8881 cctcctcgcg ggggtcggcc tgacggcctg ggtccagtcg gggctccccc gcgtcgcggt
     8941 ctccttcacg ctgggcctcg ccgcgtcggc gctcctcctc ttcctcgcgg cgcacgccct
     9001 cctccgcctc gtcgcgcgcg tcccgcgcga gcgcctcccg ttcgcgctca agagcgggct
     9061 ctccgcgctc tcccggccgg gggcggggac cgtcggctcg atcctcgcgc tcggcctcgg
     9121 gacgctcgtc gtcctccaga tcgccctcgt ccagcgcgac ttctcccgcc agctcacggc
     9181 cgacctgccg aggaacgccc cgaccgcctt cctcctcgac gtccagcccg accagtggcc
     9241 cggagtcgcg gcgctcctct cggcgaacgg ggcgacgcgg accctctcgg tccccgtcgt
     9301 aaccgcgcgc ctcacggcgg tcgacggcac gccgaccgcc gagctcgcga aacggagggc
     9361 gggggattcg cgccgggagc gctgggcgct gacgcgcgag cagcgcctca cctacatgac
     9421 cgagctcccc gaggacaacc gcgtcgtcgc gggcgcgctc tggtcggacc cctccgcggc
     9481 cgaggtgagc ctcgaggagg agttcgcggg acgaatcggc gcgaaggtcg ggacgtggct
     9541 cgacttcgac gtccagggcg tcccgctccg cctgaaggtc acgagcctcc gctccgtcga
     9601 ctggaggacg ttcgggatca acttcttcct cgtcgtcgag cccggcgtcc tcgagaaggc
     9661 gccgcagctg cgcctcgcgg ccgcgcggct cccgagcgcg cacgaaggga ggatccagga
     9721 cctcctggcg cgggagttcc cgaacgtcgt cctgatccgc acacgcgagg tcctcgagcg
     9781 ggtcgccgcg atcgcgacgc ggctcgggaa ggccgtgcgg gtcctcggcg ccttctccgt
     9841 cctcgcgggg ctcgtcatcc tcgccgggtc gatcagcgcc gcctccgccc gccgcgcgcg
     9901 cgaagcggcg ctcctgaagg tcctgggcgt cacccgcggc ggcgtcgcga ccgccctcgc
     9961 ggtcgagtac gcgctcgtcg gactcgtggc gggcctcgtc ggaacgctcg gcgcgacgct
    10021 cctcgccggc tcggtcctca cggcggcctt cgaggtctcc ttccgccctt ccccgctcct
    10081 cctcgcggcg acgccgctcg cagtggcgct cctcgcggcg gccgcgggac tcgccgtcag
    10141 cctccgggcg ctccaggtcc ggccgctcga ggtccttcgc ggagagtgag cccggagcgc
    10201 gggccgcggc ccgcccggct ccggggagag gtcagtccgc gatcgcgtcc tcgacggtcg
    10261 tcaggtcgac gacgccgagc gctccgtaga gcgccgggag gaccttcgcg gccgggtgga
    10321 gcttccgcag ctcggcgacc ttctcggtgc cggcgcggta ggccgggagg tacatccggc
    10381 cgaggagcgg gtggcgcccc caggcgcccg agagcttgtc ggcccgctcc tccgagacga
    10441 ggtagtcgtt ccggaggacg cgcgcgacgt cctccttcgc ccacccctcg ttccaggtga
    10501 gccaggagga ctggttcttc gcgtcgtcct ggaggagcgc gaggagcatc cccgcctcca
    10561 tctcgcggtc cggcagctcc tcgggagaga agaccccgta ggccatcagg agagcgttgt
    10621 tcgcgatccc ctcgaagagc gccgcgggcc gggtgttcat cgtgaggacc gacgcctcga
    10681 agccgacgat cccgcggacg aagaggtcct gcaggaacgc aaacgtcgtc acgtgcccgg
    10741 ggacgacctc gtggctgacg agctgggcga actcggggcg ggagatctgg agcgaggcgt
    10801 tcagctcgta ggtcgcctcg tactcgggcg agccgtccgg cctccgcgcc cggccgaggt
    10861 agttcatcga gcccgagaac caggcgttct cgatgggaag gaacgtcacg ttcgcccgcg
    10921 ggacgcgcgc gacctccgca gggaggaacg gcatcacgtg ccgcgccgcg agggtgtcga
    10981 gctcggccac gagacgggcc gagagggccg ggatctcctc cttcgggacg atccgctccg
    11041 cgcgccacgc gtcgacggcg gcgaggagcc ccggaccgtc cgtcgaaggg tggcccgccg
    11101 cggagaggag ctcgcggaca cgttcccgct tcgccgccgg ctcggagggc gaaccgggtc
    11161 tcccgaggat cgcgcgcatg cagcgctcgt acgggaccgg ctccccgcgg cccgcgacct
    11221 cgagggcgag gtcgagcatc acctcgaagc agaggccgag cccctcgagg tagcgggccc
    11281 ggaggcccgc gccgcgcccc gcctcgtcgc ggacgcgggc gatcgcgccg cgcacgtcga
    11341 cggcgtcgag ataggcgtcc agcgcggcga ccgtctccgg cttcgccgag gcgccgccga
    11401 gctcgcgcaa ccgggccgcc tccggcctcg tgtaggaggc cgggagcggc tcgtccgaga
    11461 accaggagtc ggcgatgaag cgccccgtcc ccgacgggtt catgacgtcg ccgccccaga
    11521 gcgtgtcgag ccccaggacg gcggtcgcga cggcctggtc gagggcgtcc acggcctact
    11581 cctccccgtt cgtccggcgg gcgtcccagt acatgtaggc cgcgaggagc acgtaggcac
    11641 cgagggcgag gagctcgggc cccatcccct ccgagaatcc gaagttccag ccgaggagcg
    11701 acgggatccc cgtcccgatc gcgcccgcga ccttctcgtc ggtcgccagg aacttgaaga
    11761 ccgtggcgac gacgcccatc accgcgccgc cggcgatgaa gccggaggcg atcaggatgc
    11821 cgcgcgagtt gcgcgcagcg gcgagcttct cgtccttccc cgcgctcttc gccacgaggt
    11881 gcgcgatgaa gccgccgacg aggagcggcg tgttcagctc gagcgggatg tacatgccga
    11941 gcgcgaacgc gagcgccggc acgccgatca tctgcatcga gatcgcgatg agcgccccgg
    12001 tgccgtagag gagccagggg accggctccg ccgacatgag cgtcttgatg acggcggcca
    12061 tcgcgttcgc ctgcggcgcc gcgaggacct gccccgccgg gtggtcgggc cccgggacga
    12121 agccgtagac cttgttgagg aggaggagga ccgagccgac ggagagcgcc gccacgatcg
    12181 tcccgaggaa cttcgagcgc tgctggatcg cgggcgtcgc cccgatccag tagcccacct
    12241 tgaggtcggt gatcaggccg cccgccatcg agagcgccgt gcagacgacg ccgccgatga
    12301 ggagcgccgc gagcatcccc gcgttgccgg agagccccgc cttcacgagg aagagggagg
    12361 tgaggatgag cgtcatgagc gtcatccccg agacggggtt cgtcccgacc gtggcgatcg
    12421 cgcgcgcggc gaccgacgtg aagaggaacg agatgacgat gacgacgagg agcgcgatga
    12481 gcgactgcgt cccgatgctc ggggaggggt cgaggacgcc gaagcggaag aacgcccagg
    12541 cgacgagggc cacggcgacg aggccgccga ggatgacctt cattgggagg tctcggtccg
    12601 tccgttcggt ctcccccgac gccgacttcg cgccgccgaa gatcgccttg aaggagaacg
    12661 ccttcgcgat gacgcgccag ctccggagga ttcccaggat cccggccgcg gcgatggccc
    12721 cgatgccgat gagccggacg tagttgcgga agacggcctc ggcgcccatc gccccgacga
    12781 gcgtcccgtc ggtgaccggc gggatgccgg tcgtggcccc cgcgccgaag tgcgcgatga
    12841 ccggcacgag gaggaaccag gagaggaacg agccggcgca gatgaccgcc gagtacttga
    12901 ggccgatgat gtagccgagc cccatgaccg aggagaggac gtcgatcttg aagacgatct
    12961 tcgaccgctc ggcgaccttg tcgaggagcc agaccgagcg cgtcgtgaag acctcggcga
    13021 aggcctgcag gtggaggatc aggaagtcga agatcccgcc gatcagggcg gcggccacga
    13081 ggaccttcgc ctgcttccct ccggcctcgc ccgcgacgag gacctccgtc gtcgccgtcg
    13141 cctccgggaa cgggaactcc ccgtgcatct ccttcacgaa gtggcgccgg agcggcacga
    13201 ggaagacgat gccgagcgcg ccgccgagga gcgagacgag gaagagcttg aagaggtcga
    13261 ccggcaggcc gaggatgaag agcgccggga gggtgaagat cgcccccgcc acgacgagcc
    13321 ccgaggccgc gccgaccgac tggacgatga cgttctcgag gaccgtcgac cggcgcttga
    13381 aggcgtagcc gaggccgacc gcgaggatgg cgatcgggat cgccgcctcg aagacctgcc
    13441 cgaccttcag gccgaggaac gccgccgcgg cggagaagac ggcggccatg acgatgccga
    13501 gcgtcacgga ccggggcgtg aactcctgga tcgcggcgtc ggcggggacg atcggctggt
    13561 aggtctcgcc cggcttcagc ggccgatagg cgttctcggg gagcgacttc gacgaggaga
    13621 cttccatcat tcctccgtgg ggaccccgca cgcggggcgg cgggcgtcgt tcgcgggcga
    13681 tctcggagtc tgatgggaaa cggcccgtcc gtcgagcggc atcgttcaac ggcccccgtc
    13741 gttccgaccc gccccggggc gggcgcggcc gccgccggct ccccgcgggc ggtcagtcgc
    13801 ccgcgaggag agccgcgatc gcttcctcgt acgggagggg ctcgaccatc ccgaggaagc
    13861 gccccgggac cccgaagaca gcctcgcgcg tccggccgta cgggttccgc tcgaagatct
    13921 cgaggacctg ccgccggagc aggggcgagg gaaccagccg ctcgaatcgc gtctcgaggg
    13981 aggagaggaa cggaaggacc gcgatccgcc cgggccgctg cgccttcatc tcggcgaaga
    14041 ggcctgcgat cgcctcgagc cgcgaggtcg agaggaagaa gtacgacccg atcatgtcga
    14101 cggcctgcat cggaagccag aacggcgcct cgtcggagac cttctcgaaa tggaagacgg
    14161 tctggagcac gaggtgctcc acgagccggg cgagcgccgg gacccggccg cacgctccgc
    14221 ccagcgcgag cagcagctcc cgggcgacgg ccgcgccccg gagctcgacg gcaggggtgc
    14281 cgtagagggc cgaggagtcg tagctcttcg cgaggtcgag gtccaacccg gacccggtca
    14341 gggcaacccg ctcggaggcg gagatgttgc cgaggtcgtg acaggcgaac gcgaagccga
    14401 aggcggtccg caacgttccg gcgtccggct ccggctcccc cgcctccacg gaccagcggc
    14461 gggcgtcccg ttcgagcccg aacgggtcga ggccctctcc gagcgccccg aacagcgcct
    14521 ccacgcagtc gcagacggcc gcgacgtgcc gcgcgttgtg gtacgagggc ggccggacga
    14581 cgccggggag gacgacctct cccgaccggg cccagacctc ggcctcgaac cgctcgtgcg
    14641 cggcgacggc gaggccccgg atctcgcgga ccaggtcggg gttgcagggg acgaacgctt
    14701 cgctctcgga aggcgtcatc gggctctcga cggaatgtta gcagcggcct cttcggagac
    14761 agcgcctcgt ctgcgcgggc cgtcgtagac tcggcgctcc tcccacgagg gaccggcgtg
    14821 aaccccgacc tcgcatacga agcgctcccc cgcctcctcg tggccgccct cggcggcctc
    14881 gccgtcggcg tcgagcggga gtggtcggct cgcgccgccg gcggagtgcg ccgcttcgcg
    14941 ggcgtccgga cgttcctcct catcggactc tccgccggga ccgcggccgc cgtctccggc
    15001 tcgggagcgg ccgcggcggg cctcctcctc ctcggctcga tcgcgctcct cgtcgtcgcc
    15061 tccttcacgg tccacgcctt cgccggcgac ctcgacgcga cgacggaggt ggccgcactg
    15121 gcggtgctcg gggcgggctg gctctcgggc tccgggcgga tcgcgctcgg aagcgccctc
    15181 gcggctctcg tcgccctcgt cctcgcggag aagacgaaga tccacggcct cgtcgagaag
    15241 ctccgctccg aggaggtggg cgccggcgcc cacttcgccg tcctcgccct cgtcgtcctg
    15301 ccgatcctgc cggccgggcc gttcgggccg tcgccgggca tccgtccgca ggagctctgg
//
LOCUS       DAOTEZ010000050        15317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2646_length_15317_cov_5.881994, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000050 DAOTEZ010000000
VERSION     DAOTEZ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15317
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2646_length_15317_cov_5.881994"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="P5164_04105"
     CDS             <1..521
                     /locus_tag="P5164_04105"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HRY43111.1"
                     /translation="TPIGFKWIAEKMLSGEVGIGGEESGGLGVSFFLPERDGVLTGLL
                     VLEAIAHSGGSFRELLERQDREHGSFSYGRRDVRLPMTVLRSFVDGLTAAPPSRRAGA
                     TVTGVETMDGVKLHFGGRGWLLHRLSGTEPILRVYAEHEDAAVVEKLLSETTEALERS
                     AQGAPSAALPAP"
     gene            complement(526..1806)
                     /locus_tag="P5164_04110"
     CDS             complement(526..1806)
                     /locus_tag="P5164_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY43112.1"
                     /translation="MSSRASETGRSAPFFDHGLFLLHLNRGKEDLRKGLYDDARREFE
                     EARRFRATDPEVLSNLALAMFHLGHLDDAERITRGLLVSHADSVPLLFNLGLILFKAG
                     RAEAREPLERVLALAPGHRKAHLTVGLLLQREGRLAEANRHFEAAGSERKAGFDGDDT
                     ITRTARSAASGPSEPGAYVPSVKPEAFDDSRTRREPSTQPIQAPQPPPEAPRSAPVPP
                     AVAPVAPPVPARILVGVPEGAQGPRPTFSPRPGGVVAVDLRDGAFVRRTALAGRTGVA
                     ELEADGSLPGALSRFFVHGTGAGGVLLVDPRRSLHLVFLRGEFLSADPARLFGFDARL
                     TYREDPAFEFRRQIALPFLKLFGSGAVAFSVAAEPARLEVSPETPLTLSCRSLLAYGG
                     ELTVDLVEDSDPLAELGGGPVVTVSGAGWVLIEA"
     gene            complement(1803..2552)
                     /locus_tag="P5164_04115"
     CDS             complement(1803..2552)
                     /locus_tag="P5164_04115"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018936551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRY43113.1"
                     /translation="MLRRAVLCVAAGLFVFAGEALSDVKMIRRADGRAVIFNSIGSGW
                     RIGGRAPSDAYLLARRDAATSFDDVIRTHAERERVDPRLVKSVMLIESNFNPRAVSRK
                     GARGLMQLMPGTARRYGVSNSFDPHDNIRGGTAYLSDLLGMFDGDLVRTLAAYNAGEG
                     AVQRYRGVPPYPETQEYVRRAMLVYGGSATGLPVLSGGFKGKSIAPHRTVSSAPIRLH
                     RGTEGLVLSNQGDPLRSGRPEPILGRVPAGR"
     gene            complement(2584..5277)
                     /gene="alaS"
                     /locus_tag="P5164_04120"
     CDS             complement(2584..5277)
                     /gene="alaS"
                     /locus_tag="P5164_04120"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRY43114.1"
                     /translation="MTTPSDWTSRKVRETFLSYFEEKGHRRVASSPLVPAEDPTLLFT
                     NAGMNQFKDVFLGRESRPYVRAASAQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFS
                     FGDYFKEEAIAFAWELVTERYGFEPKRLWATVYTDDDEAAKLWERYLPASRVLRFGEK
                     DNFWSMGDTGPCGPCSELHVDQGPHVPGDETPNGRGDRVMEIWNLVFMQFERDSEGRT
                     TPLPKPSVDTGAGLERITALLTGKNNNFDTDLFAPLIRGIEAISGKSYRGGMAEEDAP
                     FRVIADHLRAATMLMADGVLPANTGRGYVLRRILRRAARYGRRLGIEGPELCGLVEPV
                     FSVFDGVYFGAEHETLRGEVRELVRSEERSFAKTMSAGADRVGEAISEARRKEETGLS
                     GKTVFRFYDTYGIPLELIEELAQDEGLSVDRGGFEKELEEARERSKGASKFEADDALL
                     PEELAEPSWETAFRGYPEDDFTLLEGAKALGLFSVAGGSPRRVGALETGTEGLLVTDR
                     TVFYPEGGGQVTDTGTIAWPGGRAAVTSARKSASGRFVQHHVKVEEGTLAEGATLDLE
                     VSEWTRRRTQANHTGTHLLHAALRKVLGESVRQMGSLVAPDRLRFDYAATRATTPAEL
                     AEIERLVNEQVLRDTPVAKEVTSMDEAKAKGAMMFFGEKYGERVRVVDIPGFSTELCG
                     GCHVGRTGEIGAFKVLSDRGLAAGVRRIEAITSLGAVERLTRDEHLLSELSETAQVPA
                     EELPGKLREVLARLKESEKEIAKLKVQLASGTRGPAGVEEEGIVEVSGLRVLARRVPS
                     LPANELRNLADTFRGKLKSGVVLLGTEADGKVTLLAAVTADLVSRVSADDLAKAMAPV
                     VGGRGGGKPDLAQAGGKDPEKIEAALAAGVARAKEKLEASA"
     gene            complement(5261..5758)
                     /locus_tag="P5164_04125"
     CDS             complement(5261..5758)
                     /locus_tag="P5164_04125"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HRY43115.1"
                     /translation="MSGEPRRRPRRDCRTAALAFLGRRAHPRRELERKLLRGGHEAAE
                     VARTLDALAAAGLLDEAETAAELARFEAGRGRGSRRVSASLRSRGVAEADAARALAGL
                     GGESEAARLAAALEKKARSLPGGLTPVSRSRKLFDHLVRRGFDPAAVREALREKGDPT
                     DDDSL"
     gene            complement(5924..7003)
                     /locus_tag="P5164_04130"
                     /pseudo
     CDS             complement(5924..7003)
                     /locus_tag="P5164_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637334.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
     gene            7119..7718
                     /locus_tag="P5164_04135"
     CDS             7119..7718
                     /locus_tag="P5164_04135"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRY43116.1"
                     /translation="MPFGSDRVAAVLLAAGASRRFGSPKMLARVGGEPLVRRVARSFR
                     EAGFGEVAVVLAPGADEVAAALDGLGVLLAVNPRPEDGMLSSAQAGLSALSSGCGRIA
                     LTPADLPGLTAPVLRRVLGCLPPPTAAQVAVPECDGRRGHPLVIPASLAPRVLSWEPG
                     RRLSDLLGEEDVEVVPLSGFGPEVLRDVDVPADLAPALR"
     gene            complement(7798..8718)
                     /locus_tag="P5164_04140"
     CDS             complement(7798..8718)
                     /locus_tag="P5164_04140"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HRY43117.1"
                     /translation="MAFEDVKKIWMNGKLVDFADAKVHVFTHALHYGSGVFEGIRCYR
                     TARGPAVFRLDEHLDRLFWSGKMYRMEIPYTLEQLREATLETIRANGFDACYIRPLAY
                     RGFGMLGVNPFPNPVDVMIGCYPWGKYLGPEAMEKGVDVCCSSWTRLAANTMPAMAKS
                     TANYANSQLIKMEALVNGYEEGIALDDTGRVSEGSGENLFVVWKGKVYTPPFANSALP
                     GITRASVLTLFAEMGIPVEERALPREMLYAADELFFTGTAAEITPIRSVDRIVVGPGR
                     RGPLTAKVQAAFFDLVEGKVDDRFGWLTPV"
     gene            complement(8766..9476)
                     /locus_tag="P5164_04145"
     CDS             complement(8766..9476)
                     /locus_tag="P5164_04145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HRY43118.1"
                     /translation="MSAPPRVLVPLAPLEPGREVTLPEEEVRHLRVSRVGPGEELVLL
                     DGRGTRAAAHLSASGRAASVTALLPPRGEPARRVTVLLGVGEPARVEWAVEKGTECGA
                     AAFVLVAAARSQRAHVAAAAARIERLRRIAAEAVKQCDRSVVPEVGAPAPLAGLLDGR
                     RGRLLVARPGAAPLPAEQLPAGDLAVAIGPEGGFDDVEERLLDAAGAVPVGLGGRVLR
                     LETAVVAALVRLVDTSPT"
     gene            complement(9473..10282)
                     /locus_tag="P5164_04150"
     CDS             complement(9473..10282)
                     /locus_tag="P5164_04150"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRY43119.1"
                     /translation="MPAAIRLDLDPASEPALEESLGELLASWSVRDGRLSVWVSEEDA
                     GAAEELLAAAGLEVLAREVEPERDWVAEAAALQRPVAVGRFVLDPHDRMPEGVGARRR
                     LRLPAERAFGTGSHESTRLALRLLLAADPAGRSVLDVGTGAGTLALAARAAGARRAVG
                     FDLDLDAPFVARRNAFRNGVNGCAFWAGLLPSLTAHARFDLVVANMLQEEVAPLLPGL
                     AAILPPGGTLVTAGQLAAREEEFLVRLVDAGLHPRRLASEGEWLGTLSDRA"
     gene            complement(10291..11412)
                     /gene="dnaJ"
                     /locus_tag="P5164_04155"
     CDS             complement(10291..11412)
                     /gene="dnaJ"
                     /locus_tag="P5164_04155"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRY43120.1"
                     /translation="MSTTSRDLYEVLGVARDASTDQIKSAYRKLALKHHPDRNPGDKD
                     AEQKFREAAEAYSVLSDAEKRSRYDRFGAEGVRGGAGGFDPNAFVDFADILGDFFGFG
                     FGGGGRRGRQTPGEDLRADLSLTFEEAAFGAEKSLPMRRFERCDTCHGSGGKGGSGAV
                     ACGVCRGRGRVQYRQGFFAMERPCPECQGAGEKLKDPCPDCRGEGRTLRERTLTIAIP
                     AGVDTGARLRLSGEGNHGRAGGHAGDLYVVLDVKPHELFRREGYDVVLTWAVPYPTAV
                     LGGTCRVPTPHGEEELEIPAGTGAGRAFTLRGKGIPRVDGRGRGDEHVVVTIRVPKKL
                     SSEQRKAVQKLADVLKEEPGSPTREEKGFLEKLRDLFSS"
     gene            complement(11431..13257)
                     /gene="dnaK"
                     /locus_tag="P5164_04160"
     CDS             complement(11431..13257)
                     /gene="dnaK"
                     /locus_tag="P5164_04160"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRY43121.1"
                     /translation="MGKVIGIDLGTTNSCVAVIELGAPQVVSNREGARTTPSIVAFTE
                     DGERLVGQIAKRQAITNPTQTIYAVKRLIGRKWDDAEIRHAREHLAYPIVAAPNGDVK
                     IRVRDRDYASEEIAAFVLREIKEFCEEAVGEEIREAIITVPAYFNDSQRQATRDAGRI
                     AGLEVLRIINEPTAASLAYGLERGQKNETIAVYDLGGGTFDISILKIDQGMFEVLSTS
                     GDTYLGGEDFDRAVMDWLLSDFLLQTGIDLRQDRLALQRLKEAAEKAKCELSTTQETT
                     IGLPFIASDAGGPKHINKVLTRDHFEALVAGLIERTAGPCRNALEAAGLTPGEIDNVI
                     LVGGQTRTPKVQEMVESIFGKPPNRSINPDEVVAVGAAIQAGVLQGEIKDVVLLDVTP
                     LSLGVETHGGIFVKLIERNSTIPTKNSMVFTTVSDNQSVVEIHVLQGEREIARENKSL
                     GKFDLVGIPPAARGVPQIEVTFAIDSSGIVNVSARDMATGRAQGVQINPAGGLSQGEI
                     DQIIHEAEENKLADQKRKVVRQLKNKLEGQITGNERVFREFGKLLGADERDRIARTLE
                     HSREVLTSEERGEIDNALLDMQGVSRLLTAAMLYKGDKIDKD"
     gene            complement(13284..13994)
                     /gene="grpE"
                     /locus_tag="P5164_04165"
     CDS             complement(13284..13994)
                     /gene="grpE"
                     /locus_tag="P5164_04165"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRY43122.1"
                     /translation="MAENDGRPVPPDDEVVYLDEDGGSDDVASALEAAERAVAAVEER
                     HRAAPQPVRAAGEPAPLPETVEAPAGTVRASLAEERERAIRAEEEAGRLREALLRKVA
                     DFDNLRKRNEREKAEYTRYALSEVMLDLLGVLDNFERALEHAPSSVGDDFRTGVEMIA
                     RQLTEVLRKHGVVEVNAEGVPFDPQVHEAIMREETAAVPHGTVVQVFQKGYVLNDRLL
                     RAARVKVSAAPAAPAGEA"
     gene            complement(13994..15052)
                     /gene="hrcA"
                     /locus_tag="P5164_04170"
     CDS             complement(13994..15052)
                     /gene="hrcA"
                     /locus_tag="P5164_04170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HRY43123.1"
                     /translation="MGDVTRQLDHRSREVLASVVGTYIRSATPVSSRQLTGSGGFSLS
                     SASLRNAMADLEELGYLTHPHASAGRVPTDLGYRTYVRELMPSRPPGLAEKAKIETGL
                     GAGSFELERFLHATSRVLTELTGEVAVVAAPDSAHVVLRSVHFTRVAERKVVVVTVSG
                     EGLVGSRLIETRDDYSPEELQAVSNRLTADYGGKTLLEIRALLLAALQEEKVRFDAGI
                     ARALELARRVFAEERPGGETLVVEGTGTILEKPEFQRDVDSLRRMYRALEQEARLVDL
                     LTDCLAGGGQPAVLIGSDSAFTEETGTAVVVTAYWSGDRVLGALGVIGPRRMEYPRVV
                     PLVEELGRYVTRRLSEGV"
     gene            complement(15075..>15317)
                     /locus_tag="P5164_04175"
     CDS             complement(15075..>15317)
                     /locus_tag="P5164_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43124.1"
                     /translation="AVAPQQGEEALRAFAGFPTPGAPRVEAGPPKGFHECGTEPAAAD
                     DDGRKRRGDCYHVATPGGTLRTFVYGPRRRAVPCIR"
BASE COUNT         2055 a         5570 c         5431 g         2261 t
ORIGIN      
        1 tcacgccgat cggcttcaag tggatcgccg agaagatgct ctcgggcgag gtcggaatcg
       61 gcggcgagga gtccggaggg ctcggcgtct cgttcttcct ccccgagcgc gacggggtcc
      121 tcaccggcct cctcgtcctc gaggcgatcg cgcattcggg cggctcgttt cgcgagctcc
      181 tcgagaggca ggaccgcgag cacgggtcgt tctcctacgg tcggcgcgac gtccgcctcc
      241 cgatgaccgt cctccgctcg ttcgtcgacg ggctgacggc ggctccgcct tcccggcgcg
      301 ccggcgccac cgtcaccggc gtcgagacga tggacggcgt caagctccac ttcggcgggc
      361 gcggatggct cctccatcga ctctccggca ccgagccgat cctgcgtgtc tacgcggagc
      421 acgaggacgc ggccgtcgtc gagaagctcc tctccgagac gaccgaggcc ctggagcgct
      481 cggcgcaggg cgcgccgtcc gccgcgctcc cggcccctta gggcctcagg cctcgatcag
      541 gacccagccc gcgcccgaca cggtcacgac cggcccgccg cccagctcgg cgagcgggtc
      601 ggagtcctcg acgaggtcga ccgtcagctc gccaccgtac gcgaggaggg agcggcagga
      661 gagcgtcagg ggcgtctcgg gcgagacctc gagccgggcc ggctccgccg cgaccgagaa
      721 ggcgacggcc ccggacccga agagcttgag gaacgggagc gcgatctgcc ggcggaactc
      781 gaacgccggg tcctcgcgat aggtcagccg ggcgtcgaag ccgaacaggc gggccggatc
      841 ggccgagagg aactccccgc gcaggaacac gaggtggagg ctccgccgtg ggtcgacgag
      901 gaggacccct cccgctcccg tcccgtggac gaagaagcgt gaaagcgccc cggggaggct
      961 cccgtccgcc tcgagctcgg cgacgcccgt ccgccccgcg agcgcggtcc gccggacgaa
     1021 ggccccgtct ctgagatcga cggcaacgac gccgcccggt ctcggagaga aggtggggcg
     1081 gggcccctgc gcgccctccg ggacaccgac gagaatccgc gccgggaccg gcggggcgac
     1141 gggcgctacc gcgggcggaa cgggcgccga gcgcggcgcc tccggagggg gctgcggcgc
     1201 ctggatgggc tgcgtcgacg gctcccggcg ggtccgggag tcgtcgaacg cctccggctt
     1261 caccgaaggg acgtacgccc cgggctccga cgggcccgac gcggcgctcc gggcggtccg
     1321 cgtgatcgtg tcgtcgccgt cgaaccctgc cttgcgctcg gagcctgcgg cctcgaaatg
     1381 gcggttcgcc tcggccaggc gaccctccct ctggagcagg aggccgacgg tgagatgcgc
     1441 cttccgatgc cccggggcga gcgcgaggac gcgctcgaga ggctcgcgcg cctccgcccg
     1501 gccggccttg aagaggatca ggccgaggtt gaacaggagc gggaccgaat cggcgtggga
     1561 gaccaggagc ccccgcgtga tcctctcggc gtcgtcgagg tgcccgaggt ggaacatcgc
     1621 gagcgccagg ttcgagagga cctccggatc ggtcgcccgg aagcggcggg cctcctcgaa
     1681 ttcacggcgg gcgtcgtcgt agaggccctt ccggaggtct tccttgcccc tgttcagatg
     1741 gaggaggaag aggccgtgat cgaagaaggg agcgcttctg ccggtctcgg acgcgcggct
     1801 gctcatcgcc ccgccggcac gcgtcccagg atcggctccg ggcgcccgct ccggagggga
     1861 tccccctggt tcgagaggac gagaccctcc gttcccctgt ggagccggat cggggccgac
     1921 gagacggtcc ggtgcggcgc gatcgactta cccttgaacc cgcccgagag gacggggaga
     1981 ccggtggcgc ttcccccgta gacgagcatc gcccgtcgca cgtactcctg cgtctccgga
     2041 tagggaggca cgccccgata ccgctggacc gcgccctcac ccgcgttgta ggcggcgagg
     2101 gtccggacga ggtccccgtc gaacatcccg agcaggtcgg agaggtaggc ggtcccgccc
     2161 cggatgttgt cgtgcggatc gaaggagttg ctcacgccat accgccgagc ggttcccggc
     2221 atcagctgca tcagcccccg ggcccccttc cgggagaccg cgcgggggtt gaagttcgac
     2281 tcgatgagca tgacgctctt gacgaggcgc gggtccaccc gctcgcgctc ggcgtgggtc
     2341 cggatgacgt cgtcgaagga ggtcgcggcg tcgcggcggg ccaggagata agcgtccgat
     2401 ggggcgcgtc cgccgatcct ccagccgctg ccgatgctgt tgaagatcac ggcgcggccg
     2461 tccgcgcggc ggatcatctt cacgtccgaa agcgcctcgc ccgcgaagac gaagagcccc
     2521 gcggcgacac agagcacggc ccggcgcagc atctcccgcg aagtataccg cccggaggcc
     2581 ggctcaggcc gacgcttcga gcttctcctt cgcccgggcg acccccgcgg cgagcgcagc
     2641 ctcgatcttc tcgggatcct tgccgcccgc ctgggcgagg tcgggcttcc cccctccccg
     2701 tccgcccacg acgggcgcca tcgccttcgc caaatcgtcc gcggagacgc gagagacgag
     2761 gtccgccgtg acggccgcga gaagcgtcac cttcccgtcg gcttccgtcc cgaggaggac
     2821 gacgccgctc ttcagcttcc cgcggaacgt gtcggcgaga ttccggagct cgttcgcggg
     2881 gagggacggg acccgccgcg cgaggaccct gagtcccgag acctcgacga tcccctcttc
     2941 ctcgacgccg gcggggccgc gggtcccgga ggcgagctgc accttcagct tcgcgatctc
     3001 cttctcgctc tccttcagcc gcgcgaggac ctcccgcagc ttcccgggga gctcctcggc
     3061 cggaacctgg gcggtctcgg acagctcgga gaggaggtgc tcgtcccgcg tcagccgctc
     3121 gacggctccg agcgaagtga tcgcctcgat ccgccggact ccggccgcga ggccgcgatc
     3181 ggagaggacc ttgaaggcgc cgatctcgcc cgtgcggccg acgtggcagc cgccgcagag
     3241 ctcggtcgag aagccgggga tgtcgacgac gcggacccgc tcgccatact tctccccgaa
     3301 gaacatcatc gcgcccttcg ccttcgcctc gtccatggag gtgacctctt tcgcgaccgg
     3361 cgtgtcgcgg aggacctgct cgttgacgag gcgctcgatc tcggcgagct cggcgggcgt
     3421 cgtggcgcgc gtggcggcgt agtcgaagcg gaggcggtcg ggcgcgacga gggagcccat
     3481 ctggcggacc gactctccga gcaccttgcg gagcgcggcg tggaggaggt gcgtcccggt
     3541 gtggttcgcc tgcgtccggc ggcgggtcca ctcggagacc tcgaggtcga gcgtcgcgcc
     3601 ctccgcgagc gttccctcct cgaccttcac gtggtgctgg acgaagcggc cgctggccga
     3661 cttccgcgcc gacgtcaccg ccgcgcggcc gcccggccac gcgatcgtgc cggtgtcggt
     3721 cacctggccg cccccctcgg ggtagaagac ggtgcggtcg gtgacgagga gcccctcggt
     3781 gcccgtctcg agagcgccga cgcggcgcgg cgagcctccg gcgacggaga agagcccgag
     3841 cgccttcgcc ccctcgagga gcgtgaagtc gtcctcgggg tagccgcgga acgcggtctc
     3901 ccaggacggc tcggcgagct cctccggaag gagcgcgtcg tcggcctcga acttcgacgc
     3961 ccccttcgag cgctcccgcg cctcctcgag ctccttctcg aagccgcccc ggtcgacgga
     4021 gagcccctcg tcctgggcga gctcctcgat cagctcgagc gggatgccgt acgtgtcgta
     4081 gaagcggaag acggtcttcc cggagaggcc cgtctcttcc ttccggcgcg cctcgctgat
     4141 cgcctcgccc acgcggtcgg cgccggcgga catcgtcttc gcgaagctcc gctcctcgga
     4201 gcggacgagc tccctcacct cgcccctcag ggtttcgtgc tccgcgccga agtagacccc
     4261 gtcgaagacg gagaagaccg gctcgacgag cccgcagagc tcgggcccct cgattccgag
     4321 ccggcggccg tagcgcgcgg cgcggcggag gatccggcga aggacgtagc cgcgccccgt
     4381 gttcgccggc aggactccgt cggccatcag catcgtcgcg gcacgcagat ggtcggcgat
     4441 gacgcggaag ggggcgtcct cctcggccat gccgccccga tagctcttcc ccgagatcgc
     4501 ctcgatcccg cggatcagcg gcgcgaagag gtccgtatcg aagttgttgt tcttccccgt
     4561 caggagcgcc gtgatccgct cgaggcccgc gcccgtgtcg acggacggct tcggcagcgg
     4621 cgtcgtcctc ccctcggagt ccctctcgaa ctgcatgaag acgaggttcc agatctccat
     4681 gacccggtcc ccgcgcccgt tcggcgtctc gtcgcccggg acgtgcggcc cctggtcgac
     4741 gtggagctcc gagcaggggc cgcaggggcc cgtgtcgccc atcgaccaga agttgtcctt
     4801 ctcgccgaag cggaggacgc gcgaggccgg gaggtagcgc tcccagagct tcgcggcctc
     4861 gtcgtcgtcg gtgtagaccg tggcccacag gcgcttcggc tcgaagccgt agcgctccgt
     4921 cacgagctcc caggcgaagg cgatcgcctc ctccttgaag tagtcgccga aggagaagtt
     4981 cccgagcatc tcgaagaacg tgtggtggcg cgccgtgtac ccgacgttgt cgaggtcgtt
     5041 gtgcttgccg cccgccctca cgcacttctg cgcggaggcg gccctcacgt acggccggct
     5101 ctcccgcccc aggaagacgt ccttgaactg gttcatcccc gcgttcgtga agaggagcgt
     5161 cgggtcctcg gcggggacga gcggggagga ggcgacgcgg cggtgcccct tctcctcgaa
     5221 gtaggaaagg aacgtctcgc ggaccttccg ggaggtccag tcagagggag tcgtcatccg
     5281 tgggatcgcc tttctctcgc agcgcctcgc ggacggccgc ggggtcgaag ccgcggcgca
     5341 cgagatggtc gaagagtttc ctcgacctcg aaacgggcgt caaaccgccc gggaggctcc
     5401 gcgccttctt ctcgagcgcg gccgcgagcc gcgccgcctc gctctcgcct ccgaggcccg
     5461 cgagggctcg ggccgcgtcc gcctcggcga cgccgcggga gcgcagggag gcggagaccc
     5521 gccggctccc ccgcccgcgc cccgcctcga accgggcgag ctcggccgcc gtctcggcct
     5581 cgtcgaggag gcccgccgcg gcgagggcgt cgagtgtccg cgccacctcc gcggcctcgt
     5641 gcccgccccg gaggagcttg cgctcgagct cccgcctcgg gtgggcgcgc cgcccgagga
     5701 atgcgagcgc cgccgttcgg cagtcgcggc gcggccggcg gcgcggctcg ccgctcacga
     5761 ccggagccgc cgccgggtcc gccctcgggg cgcgacgcgc actgggaacg gccttcccgg
     5821 agagacgcta ggtctggttc gagaagtcga agggggactc ctctcccagc gggttcatct
     5881 tgtcgggcgc ctcgagcgac gcgtccatct cctcctgcga gaggtcggac gtcgactgga
     5941 cgccctggat gcggagcttg aactcgttcg ggttcgtcga gcggcggatc gcctcgtcga
     6001 gcgtgatgag ccccgacttg tagagctgga agagcgactg gtcgaacgtc tgcatcccgt
     6061 actgcgacgt ccccgccttg atggcgtcga cgatgtactt cgtcttctcc ttgttctcga
     6121 tgcagtcgcg gatgtagttc gtcgcgatca ggacctcgac ggccgggacg cggccgaggc
     6181 cgtccgcgcg cgggaggagg cgcatcgaga cgacggcctt gaggacggcc gacagctgga
     6241 ggcggatctg cttctgctgg tgcggcggga agaccgcgat gatgcggttg atcgtctccg
     6301 tcgcgtccat cgtgtggagc gtcgagagga cgagatgccc cgtctcggcc gccacgagcg
     6361 ccgtctcgac cgtctcgaag tcgcgcatct cgccgacgag gatgacgtcg gggtcctggc
     6421 gcagcgaggc ccggagcgcc gaggagaacg agcgcgtatc gacctccagc tcgcgctggt
     6481 tgacgatcga cttcttgtcg cggtggagga actcgaccgg gtcctcgacc gtcatgatgt
     6541 gctcggcccg cgtcgagttg atgaagtcga tcatcgccgc gaggctcgtc gacttgcccg
     6601 agcccgtcgt cccggtgacg aggatgaggc cgcgcgtctc ctcgcagatc ttctcgatga
     6661 cgggcggcag caggagatcg cggacggtca ggatgcgcgc cgggatgacg cggcagacga
     6721 ggccgaccgt gccgcgctgc tggaagatgt tgcaacggaa ccgcccgagc cccggcaccg
     6781 agtacgcgat gtcgatctcg aagttgttct tgaacttctc cttctgacgg gacgacatga
     6841 tcgagaacgc catcgcgatc gtgtcttcct gcatcaggcg cttcatgtcg acgaggggga
     6901 tgaggtcgcc gtcgacccgg atgatcgggt gcgcgccgac cttcagatgg aggtccgagg
     6961 ccttgcgctc gaccgcgatc ttgaggaggt cgttgatgtg catgccccag gtctcctctc
     7021 tacccgtccg ccgaaagtgt cttagtgtct cgaggatagg ctcgccgccc ggcccggtca
     7081 acgatcggac ggacgttccc gcggggtacc atcgcccgat gccgttcggt tcggacaggg
     7141 tcgccgccgt cctcctcgcc gccggagcct cgcgcaggtt cgggagcccc aagatgctcg
     7201 cccgggtcgg gggggagccg ctcgtccgcc gcgtcgcccg gtccttccgg gaggccggct
     7261 tcggagaggt cgccgtcgtt ctcgcccccg gcgcggacga ggtcgccgcc gccctcgacg
     7321 gcctcggcgt cctcctcgcg gtcaaccctc ggccggaaga cgggatgctc tcctccgcgc
     7381 aggcgggcct ttcggcgctc tcctcgggat gcggccggat cgccctcacc ccggccgacc
     7441 ttccgggact gaccgcgccc gtgctccgcc gcgtcctcgg gtgcctcccc cctccgaccg
     7501 cggcacaggt cgcggttccg gagtgcgacg gccggagagg ccacccgctc gtcataccgg
     7561 cgagcctcgc gccgcgcgtc ctctcctggg agccgggccg gcgcctctcc gacctcctcg
     7621 gagaggagga cgtcgaggtc gtccccctct ccggcttcgg accggaggtc ctccgggacg
     7681 tggacgtgcc ggccgacctc gccccggccc tccgatgacg cggcgcgccg gcccgtcacg
     7741 ggaccggcgc gccgcgaagg gccgacgctt cggccggccc cggacgggcc tcgcggctca
     7801 gaccggcgtc agccagccga accggtcgtc caccttgcct tcgacgaggt cgaagaacgc
     7861 cgcctggacc ttcgccgtca gcggccccct ccggcccggc ccgacgacga tccggtcgac
     7921 cgagcggatc ggcgtgatct cggccgccgt ccccgtgaag aagagctcgt cggccgcgta
     7981 gagcatctcc cgcgggaggg cgcgctcctc caccgggatg cccatctcgg cgaagagcgt
     8041 caggacggag gcacgcgtga tccccggaag ggcggagttc gcgaagggcg gggtgtagac
     8101 cttccccttc cagacgacga agaggttctc gcccgacccc tcggagacgc gccccgtgtc
     8161 gtcgagcgcg attccctcct cgtagccgtt gacgagcgcc tccatcttga tgagctggct
     8221 gttcgcgtag ttcgccgtcg acttcgccat ggccggcatc gtgttcgccg cgaggcgggt
     8281 ccaggaggag cagcagacgt cgacgccctt ctccatcgcc tccgggccga ggtatttccc
     8341 ccacgggtag cagccgatca tcacgtcgac cgggttcggg aacgggttca cgccgagcat
     8401 cccgaagccg cggtaggcga gcgggcggat gtagcaggcg tcgaagccgt tcgcccggat
     8461 cgtctcgagc gtcgcctccc gcagctgctc gagcgtgtac gggatctcca tccggtacat
     8521 cttcccggac cagaagaggc ggtcgaggtg ctcgtcgagg cggaagacgg ccgggccgcg
     8581 ggccgtccgg tagcagcgga tcccctcgaa cacccccgag ccgtaatgga gagcgtgtgt
     8641 gaagacgtgg actttcgcgt ccgcgaagtc gacgagcttc ccgttcatcc agatcttctt
     8701 gacgtcctcg aaggccatgg ctcctcctcg cggcaatgat atccgcgcgc cggaggcgcc
     8761 ccggctcagg tcggggaggt gtcgaccagg cggacgagcg cggcgaccac ggccgtctcg
     8821 aggcggagga cccggccgcc gaggccgacg ggaacggccc cggcagcgtc gaggagccgc
     8881 tcctcgacgt cgtcgaatcc cccctcgggg ccgatggcga ccgcgaggtc gcccgccggc
     8941 agctgctccg ccgggagcgg agcggcgccg gggcgggcga ccaagagccg cccgcggcga
     9001 ccgtcgagga gtccggcgag cggcgccggc gcaccgacct cggggacgac gctccgatcg
     9061 cactgcttga ccgcctcggc cgcgatccgg cgaagcctct cgatccgggc ggcggccgcc
     9121 gcgacgtgcg ctcgctggga gcgcgccgcg gcgacgagga cgaacgcggc ggccccgcac
     9181 tccgtcccct tctcgaccgc ccactcgacc cgggcgggct cgccgacgcc gagaaggacg
     9241 gtgacgcgcc gggcgggctc cccgcgcggc gggaggagcg ccgtcacgga ggccgcgcgg
     9301 cccgacgccg acaggtgcgc cgccgccctc gtgcctcggc cgtcgaggag gacgagctcc
     9361 tctcccggtc cgacccggga gacgcgaagg tggcggacct cctcttcggg cagcgtcact
     9421 tcccggcccg gctccagcgg ggcgagcggg acgaggaccc gcggcggagc gctcacgccc
     9481 gatccgagag cgtcccgagc cactccccct cggaggcgag gcggcgcggg tgcaggccgg
     9541 catcgacgag acggacgagg aactcctcct cccgagcggc gagctggccg gcggtcacca
     9601 gcgtccctcc gggcgggagg atcgccgcga ggccgggcag gagcggcgcg acctcttcct
     9661 ggagcatgtt cgccacgacg aggtcgaaac gggcgtgcgc cgtgaggctc gggaggagcc
     9721 ccgcccagaa cgcgcagccg ttcaccccgt tccggaacgc gttcctcctc gccacgaagg
     9781 gggcgtcgag gtcgaggtcg aagccgacgg cccgtcgggc tcccgcggcg cgggcggcga
     9841 gggcgagcgt cccggcgccg gtcccgacgt cgaggacgga ccggccggcc gggtccgccg
     9901 cgaggaggag gcggagggcg agccgcgtcg actcgtgcga gccggtgccg aaggccctct
     9961 cggcggggag ccggagccgg cgacgcgcgc cgacgccctc gggcatccgg tcgtgcgggt
    10021 cgaggacgaa ccgtccgacg gcgacggggc gctggagcgc ggccgcctcg gccacccagt
    10081 cccgctccgg ctcgacctcg cgcgcgagga cctcgagccc ggccgcggcg aggagctcct
    10141 ccgccgcgcc ggcgtcctct tccgagaccc agacggagag gcgcccgtcc cggacgctcc
    10201 aggatgcgag gagctcgccg agcgactcct cgagcgccgg ctcggaggcg ggatcgaggt
    10261 cgagacggat cgcggcgggc acggccgtgc tcaggaggag aagaggtccc ggagcttctc
    10321 gaggaacccc ttctcctcgc gcgtcggcga gccgggctcc tccttgagga cgtccgcgag
    10381 cttctggacc gccttgcgct gctcggagga gagcttcttc gggacccgga tcgtgacgac
    10441 gacgtgctcg tcaccgcggc cccggccgtc gacgcgcggg atccccttcc cgcgcagcgt
    10501 gaacgcccgg ccggcgccgg ttcccgccgg gatctcgagc tcctcctccc cgtgcggcgt
    10561 cggcacgcgg caggtgccgc cgaggaccgc ggtcgggtac ggcaccgccc acgtcaggac
    10621 gacgtcgtac ccctcgcgcc ggaagagctc gtggggcttc acgtcgagga cgacgtagag
    10681 gtcgccggcg tgtcctcccg cacgtccgtg gttcccctcc ccggagagcc ggagccgagc
    10741 cccggtgtcg acgccggcgg ggatcgcaat cgtcagcgtc cgctcgcgga gcgtccggcc
    10801 ctcgccgcgg cagtcgggac aagggtcctt cagcttctcc ccggccccct ggcactcggg
    10861 gcaggggcgc tccatcgcga agaagccctg gcggtactgg acgcggcccc ggccccggca
    10921 gactccgcag gcgacggcgc cgctcccccc ctttcctccg ctcccgtgac aggtgtcgca
    10981 gcgttcgaac cgcctcatcg ggaggctctt ctcggcgccg aacgccgcct cctcgaacgt
    11041 gagggagaga tcggcccgga ggtcctcgcc gggcgtctgc cgcccgcggc gtcccccgcc
    11101 gccgaagccg aagccgaaga aatcgccgag gatgtcggcg aagtcgacga acgcgttcgg
    11161 gtcgaagccg cccgctccgc cgcgaacccc ttccgccccg aagcggtcgt agcgcgagcg
    11221 cttctcggcg tcggagagga ccgagtaggc ctcggccgcc tcgcggaact tctgctcggc
    11281 gtccttgtct cccgggttcc ggtccgggtg gtgcttcagc gcgagcttgc ggtaggcgga
    11341 cttgatctgg tccgtcgagg cgtcacgggc gacgccgagg acctcgtaga ggtcgcggga
    11401 ggtcgtgctc aactcggccc tttcgagagg ctagtccttg tcgatcttgt cgcccttgta
    11461 gagcatcgcg gcggtcagca ggcgcgagac gccctgcatg tcgaggagcg cgttgtcgat
    11521 ctcgccgcgc tcctcggacg tcaggacctc gcgggagtgc tcgagggtcc gcgcgatccg
    11581 gtcgcgctcg tcggcgccga ggagcttccc gaactcgcgg aagacccgct cgttgccggt
    11641 gatctgcccc tcgagcttgt tcttcagctg cctcacgacc tttcgcttct ggtcggccag
    11701 cttgttctcc tcggcctcgt ggatgatctg gtcgatctcc ccctgggaga ggccgcccgc
    11761 cgggttgatc tggaccccct gcgcgcgccc cgtcgccatg tcgcgggccg agacgttgac
    11821 gattcccgag gagtcgatcg cgaacgtcac ctcgatctgc ggcacgccgc gcgccgcggg
    11881 cgggatgccg acgaggtcga acttgccgag cgacttgttc tcccgggcga tctcgcgctc
    11941 accctggagg acgtggatct cgacgaccga ctggttgtcg ctgaccgtcg tgaagaccat
    12001 cgagttcttc gtcgggatcg tcgagttccg ctcgatcagc ttgacgaaga tcccgccgtg
    12061 ggtctcgacg ccgagcgaga gcggcgtcac gtcgaggagg acgacgtcct tgatctcccc
    12121 ctgcaggacc ccggcctgga tcgccgcgcc gacggcgacg acctcgtcgg ggttgatcga
    12181 ccggttcggg ggcttcccga agatcgactc gaccatctcc tggaccttcg gcgtccgggt
    12241 ctggccgccg acgaggatga cgttgtcgat ctcgccgggc gtcaggcccg ccgcctcgag
    12301 ggcgttgcgg caaggacccg cggtccgctc gatcagcccg gccacgagcg cctcgaagtg
    12361 gtcgcgcgtc aggaccttgt tgatgtgctt cggcccgccc gcgtcggagg cgatgaacgg
    12421 gaggccgatc gtcgtctcct gcgtcgtgga gagctcgcac ttggccttct cggccgcctc
    12481 cttgaggcgc tgcagggcga gccggtcctg gcggaggtcg atccccgtct ggaggaggaa
    12541 gtccgagagg agccagtcca tcacggcgcg gtcgaagtcc tcgccgccga ggtaggtgtc
    12601 ccccgaggtc gagaggacct cgaacatccc ctggtcgatc ttcaggatcg agatgtcgaa
    12661 cgtccccccg ccgaggtcgt agaccgcgat cgtctcgttc ttctgcccgc gctcgaggcc
    12721 gtaggcgagc gacgcggccg tcggctcgtt gatgatccgg aggacctcga ggcccgcgat
    12781 ccggcccgcg tcgcgcgtgg cctggcgctg ggagtcgttg aagtaggccg ggacggtgat
    12841 gatcgcctcc cggatctcct ccccgaccgc ttcctcgcag aactccttga tctcgcggag
    12901 gacgaaggcg gcgatctcct ccgaggcgta gtcccggtcg cggacgcgga tcttcacgtc
    12961 tccgttcggg gcggcgacga tcgggtaggc gaggtgctcg cgcgcgtggc ggatctcggc
    13021 gtcgtcccac ttccggccga tcaggcgctt caccgcgtag atcgtctggg tcgggttcgt
    13081 gatggcctgc cgcttcgcga tctgcccgac gaggcgctcc ccgtcctcgg tgaaggcgac
    13141 gatcgacggc gtggtccggg ccccctcgcg gttcgagacg acctgcggcg cgccgagctc
    13201 gatgacggcc acgcacgagt tcgtcgtgcc gaggtcgatg ccgatgacct tccccactct
    13261 ctacctcccg gccccgcgca agctcacgcc tctccggcgg gagcggccgg ggccgccgag
    13321 accttcaccc gggcggctcg aaggagcctg tcgttcagga cgtacccctt ctggaagacc
    13381 tggacgacgg tgccgtgcgg gaccgccgcg gtctcctccc tcatgatcgc ctcgtggacc
    13441 tgcgggtcga acggaacgcc ctcggcgttc acctcgacca cgccgtgctt ccggaggacc
    13501 tccgtgagct ggcgggcgat catctcgacg cccgtccgga agtcgtcccc gacggacgac
    13561 ggggcatgct ccagcgcgcg ctcgaagttg tcgaggactc cgaggaggtc gagcatgacc
    13621 tccgagaggg cgtagcgcgt gtactcggcc ttctcgcgct cgttccgctt ccggaggttg
    13681 tcgaagtcgg ccaccttccg caggagcgcc tcccggagcc gaccggcctc ctcctcggcc
    13741 cggatcgcgc gctcgcgctc ctcggccagg gaggctcgga cggttccggc gggcgcttcg
    13801 accgtctccg ggagcggcgc gggctccccg gcggcccgga ccggctgggg cgccgcccgg
    13861 tgccgctcct cgaccgcggc gaccgcccgt tccgcggcct ccagcgcgct cgcgacgtcg
    13921 tcgctcccgc cgtcctcgtc caggtagacg acctcgtcgt ccggcgggac ggggcgtccg
    13981 tcgttctcgg ccatcacaca ccctccgaaa ggcgcctcgt cacgtagcgc ccgagctcct
    14041 ccacgagcgg gacgacgcga ggatattcca tcctcctcgg accgatgacg ccgagcgcgc
    14101 cgaggaccct gtccccgctc cagtacgccg tcacgacgac cgccgtcccc gtctcctcgg
    14161 tgaacgcgga gtccgacccg atgaggaccg ccggctgccc gcctcccgcg aggcagtcgg
    14221 tgaggaggtc gacgagccgc gcttcctgct cgagcgcccg gtacatccgg cggagcgaat
    14281 ccacgtcccg ctggaactcg ggcttctcga ggatcgtgcc cgtcccctcg acgacgagcg
    14341 tctccccgcc cggcctctcc tcggcgaaga cacgccgcgc gagctcgagg gcgcgcgcga
    14401 ttccggcgtc gaaccggacc ttctcctcct gcagcgcggc caggagcagc gcgcggatct
    14461 ccaggagcgt ctttccgccg tagtccgcgg tgagccggtt cgagacggcc tggagctcct
    14521 cgggcgaata gtcgtcccgc gtctcgatca gccggctccc gacgagcccc tcgcccgaga
    14581 ccgttacgac gacgaccttc cgctccgcca cccgcgtgaa atggaccgac cggaggacga
    14641 cgtgcgccga gtcgggggcc gcgacgaccg cgacctcccc cgtcagctcg gtcaggacgc
    14701 gcgaggtggc gtggagaaac cgctcgagct cgaaggaccc ggcgccgaga ccggtctcga
    14761 tcttcgcctt ctcggccagg ccgggcggcc gggagggcat cagctcccgg acgtacgtcc
    14821 gatagccgag gtccgtcggg acccgccccg cggacgcgtg cgggtgggtc aggtagccca
    14881 gctcctcgag atcggccatc gcgttccgga gggacgccga cgagagcgag aaccctcccg
    14941 aacccgtcag ctggcgcgac gaaacggggg tggcgctccg gatgtacgtg ccgaccaccg
    15001 atgcgagcac ctcgcgggac ctgtggtcga gctggcgagt gacgtcgccc atcccgtgcg
    15061 attctcctcc tgagctaacg tatgcaaggc acggcccggc gcctcggccc gtagacgaac
    15121 gtccggagcg tgccccctgg cgttgcgaca tgatagcagt cgcccctccg ctttctcccg
    15181 tcgtcgtcgg ccgcggccgg ctcggtgccg cactcgtgga agcccttcgg cgggccggcc
    15241 tcgacccgcg gagcgccggg ggtcgggaat cccgcgaacg cccggagcgc ctcctccccc
    15301 tgctgcgggg cgacggc
//
LOCUS       DAOTEZ010000051        15224 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2669_length_15224_cov_6.222493, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000051 DAOTEZ010000000
VERSION     DAOTEZ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15224
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2669_length_15224_cov_6.222493"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..539
                     /locus_tag="P5164_04180"
     CDS             87..539
                     /locus_tag="P5164_04180"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HRY43125.1"
                     /translation="MKRLSYCLLSALLIGAAAVPVQAVTPRQCVRKVADSTLSCTYTL
                     IAMRSLQTAVEAYAIDKHQYPVAQSITELKSLVQPTYIVTTPLTDKWGTEFRYVLSAD
                     GKSYRIVSAGSDKVFDDSSWGSPGLLESSKEDAVVSSNGRDREWAIQE"
     gene            726..1010
                     /locus_tag="P5164_04185"
     CDS             726..1010
                     /locus_tag="P5164_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43126.1"
                     /translation="MLAALRDAAIPAVDVYYSDTGEAPTPGQTSFTLWLILVPTEVLP
                     AARSVIAPLPVSRTDAPAAPVAPASVRAALVVLAIVAAFLLLLVSFLGGA"
     gene            1208..1456
                     /locus_tag="P5164_04190"
     CDS             1208..1456
                     /locus_tag="P5164_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43127.1"
                     /translation="MSYFGSTDSDGILVIPKGTLTVPGHLVLLFCWDSRSEACTAVRL
                     DSGAIATYDWVNVSLPVNRLTRRSQAHPAPQATPLPSP"
     gene            1561..2049
                     /locus_tag="P5164_04195"
     CDS             1561..2049
                     /locus_tag="P5164_04195"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRY43128.1"
                     /translation="MTTRATDQTTAGVPFPPPLAFLATLGLGFALHLLFPLHLGRTTV
                     LIEALERTGTALALAALVLAGSAFASLRRARTSPFPERPTTALVVGGPFRFTRNPLYL
                     AMALLHAGIATFSNALLPLLCLVPALVAIRFLVIPREERYLRLRFGAEYEAYCRSVRR
                     WL"
     gene            2162..2521
                     /locus_tag="P5164_04200"
     CDS             2162..2521
                     /locus_tag="P5164_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRY43129.1"
                     /translation="MLSRLIPMLPVRHVPASVAFYGKLGFTVETRHEEWGWAMLRLGE
                     CRLMVDQSINVHPDAPRDPVLYLYPDDILEYHEAVRRSGLDIPDLEVTFYGMTEFRID
                     DPDGNRLWIGQNVSGAE"
     gene            2596..3063
                     /locus_tag="P5164_04205"
     CDS             2596..3063
                     /locus_tag="P5164_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013983313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY43130.1"
                     /translation="MSLRIQEVPAAEAPLELLLLADPSEEKIRSYLPESRCFVASRGA
                     VVVGACVVQPRGAGAHELMSIAVLPEHQKSGYGTALLRWVVDLYRRAGARQLEVGTGT
                     FGYQLAFYQRQGFRVTSIDRDFFVRSYPEPIFENGIQLFDMLRLTLRYPGNVA"
     gene            3170..3673
                     /locus_tag="P5164_04210"
     CDS             3170..3673
                     /locus_tag="P5164_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43131.1"
                     /translation="MRNRLSIALLSAAAVVLASQGCVSTVFRPSPDRDIVACPAVEPS
                     SVRVLSRPPQEPFTVLGEVEAYVSGYYSDEEIVSRLRAKAAKKGAHAIYFARDVSMET
                     AERWTTGEYDTPLNDRTWRKRSSLVFRAVLLSDAPEPCPGVGRPAPVRGGATTAAPAV
                     APRSSGL"
     gene            3708..4289
                     /locus_tag="P5164_04215"
     CDS             3708..4289
                     /locus_tag="P5164_04215"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HRY43132.1"
                     /translation="MIITETFEPRSRRDWRKWLGRHHAESKAIWVVFLKKHTGRAAFT
                     YQDALEEALCFGWIDGVRQRIDDLRFAQRFTPRTKSSRWSETNRALAADLEARGLMTP
                     AGLAARGSAPPDSPAAREPASGVPEWLVDALRTSPLAWKSFNALPPSHQRRYVGWISS
                     AKRDETRNKRVAEAIALLEENKRLGLGPGEVRK"
     gene            4340..4699
                     /locus_tag="P5164_04220"
     CDS             4340..4699
                     /locus_tag="P5164_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43133.1"
                     /translation="MNARAVLASALIAAGLLATACSDTPTEPSSPVANGNLQIQVTQP
                     CALSGSVTVAANGRQLGTLAVPGESTFSVPAGSYSLAFVRGQDVFAGPGPVQVPAGGT
                     VVVTDPAGACMAPTGAP"
     gene            4843..5388
                     /locus_tag="P5164_04225"
     CDS             4843..5388
                     /locus_tag="P5164_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HRY43134.1"
                     /translation="MKTSEREELLHVLKGRFEKHGNRHPGLEWAKVLARLDGNLPALR
                     SLLAMESSGGEPDVLARGGERGPYTFYDCSAESPAGRRSLCFDGEARRARKEHRPESS
                     AVEMAAAMGIELLTEEEYRTLQGVGEFDTKTSSWIATPPDVRALGGALFGDRRYGRVF
                     AYHNGASSYYAARGFRGLLRV"
     gene            5417..5953
                     /locus_tag="P5164_04230"
     CDS             5417..5953
                     /locus_tag="P5164_04230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HRY43135.1"
                     /translation="MQILAVSGSLREASSNTTLLRALATLAPADTAVGFPVPLDSLPF
                     FSPDVEEAGLPPSVQAWRADVARADAVVVSSPEYAHGVSGVLKNALDWLVGGIEMSGK
                     PVSVLNARPQATIAHGALVETLRVMGARVVDETAVPLAGRKLDHAGIAADPELAAMLR
                     TVLSTLRKAAEDRDAGPA"
     gene            6041..6700
                     /locus_tag="P5164_04235"
     CDS             6041..6700
                     /locus_tag="P5164_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRY43136.1"
                     /translation="MSTDPLASRLAFLREVDGLKSVVRQSPLLDGSRRENSAEHSWHL
                     ALYALVLHDLAAGPVDASRVIRMLLVHDIVEVDAGDTPLHAGVPPREQAEREEAAAER
                     IFGILPRREGEELLALWREFEAAETADAAFAKALDRVQPLIANVATGGGTWLASRLTR
                     DQVLARYGPTIEKGSPRLWAACEALVRSHFSGRPDASDETGAGAGADPSATDARDHEG
                     G"
     gene            6703..7182
                     /locus_tag="P5164_04240"
     CDS             6703..7182
                     /locus_tag="P5164_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRY43137.1"
                     /translation="MVPKNTICLWYDGTALEAAQFYARTFPDSAVGKVYRAPADYPSG
                     KEGDVLTVEFTVIGIPCLGLNGGPAFRPSEAFSFQVATDDQAETDRLWDAIVDNGGQE
                     SACGWCKDRWGLSWQITPRALTAAIADPDPAVARRAFEAMMQMKKIDIAAIEAARRG"
     gene            7647..8405
                     /gene="map"
                     /locus_tag="P5164_04245"
     CDS             7647..8405
                     /gene="map"
                     /locus_tag="P5164_04245"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014993634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRY43138.1"
                     /translation="MTVENERDVEGILKAGRVVATVRDAMLEKVEPGMTTAELDEIGG
                     RLLERHGARSAPRVTYGFPGSTCISVNEEAAHGIPGPRVIRAGDVVNVDVSAELEGYF
                     ADTGGTTVVPPVSDANGSLCHATRVALDSALSEARAGAPLNRIGRAVERVARAHGFRV
                     IRNLAGHGVGRKLHEEPEGIVSYYDRGDGRRLRFGQVIAIEPFLSTRSAHVTEADDGW
                     TLVGHPENRSAQFEHTIIVTNGEPIVATRSERSG"
     gene            8536..9024
                     /locus_tag="P5164_04250"
     CDS             8536..9024
                     /locus_tag="P5164_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43139.1"
                     /translation="MSTVRIALANVRVPATPNDSVHLAIAAVAEAGRRGALVICHEGR
                     RHPEAVRWAVRVVARSNRLRRREVFVRYLLGVGGVMLLQAGLSFAIIRATAGGGSFVG
                     LGVMLAAVPGIPLTALVNALLVRSSRKDPAAACARRVVLASLALPAVQLALLALVSAF
                     RL"
     gene            9219..9803
                     /locus_tag="P5164_04255"
     CDS             9219..9803
                     /locus_tag="P5164_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRY43140.1"
                     /translation="MRASVFVGTSVDGFLARADGTFDFLSAGGSGEEPHGFEEFFATV
                     DALVMGRSTYEVVLPFPEWPYGAKPVFVLSHRPLPPEPAGAVVERMAGEPAEVFSQLA
                     ARGFGHVYVDGGRTIQAFLRAGLVERLVISRVPVLVGEGIPLFGPLDADVLLTHVATR
                     TFAGGLVQSEYEVARSPEADPQAAPTRYSPGHPP"
     gene            9800..10159
                     /locus_tag="P5164_04260"
     CDS             9800..10159
                     /locus_tag="P5164_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRY43141.1"
                     /translation="MNVVNLKERLSRFATYWDPKIVGELNGQHVKLAKMKGPFVWHSH
                     EAEDELFLVLDGSLTMEFRDRTVPLRKGELLIVPRGVEHRPVAEEEVHVLLFEPASTV
                     NTGSAGGPLTVAEPERL"
     gene            10239..10826
                     /locus_tag="P5164_04265"
     CDS             10239..10826
                     /locus_tag="P5164_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRY43142.1"
                     /translation="MELRNVCVYCGSSAGRDPAYVAAAGALGRALVSRGIGLVYGGAS
                     VGVMGAVADAVLGLGGRAVGVIPEALVRKEIAHGGLTELVVTSSMHERKTRMAELSDA
                     FVALPGGIGTLEEIFEAWTWGQLGLHAKPFGFLNVSGYWDGLVSFLDHAVRERFVREP
                     HRAMLVVAEDPEELLDRFAAWRAPEVPKWIRKGDE"
     gene            10964..11686
                     /locus_tag="P5164_04270"
     CDS             10964..11686
                     /locus_tag="P5164_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43143.1"
                     /translation="MALHAAIQALLGAAGVTNGMPGWAILESARLLPPTPGSGAAFGD
                     APAQALRKTMKAYYEYGRNHWTWAQSAGSAAANGGLVKGQIQAVACGSFNQNFKWLAE
                     KGLGITGITNGQETGQFLTMPGSVCIDSKWVGNVRTSTKAFSTLKCFKFSGHYWVMHG
                     GVNYDVCYDNTFHAVSEIIWTRLLPPDQSLVGKGGLTANQLYRLEKPLPEGDHLVMLQ
                     QNGPNGWPSWQIVPKAQIKSLK"
     gene            complement(11758..13581)
                     /locus_tag="P5164_04275"
     CDS             complement(11758..13581)
                     /locus_tag="P5164_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HRY43144.1"
                     /translation="MRPARLNARSALLAALALVLAAPLAAEERPLVPIQPDHWTKAEL
                     NNFRGTPSYDETLAFLRRLEKTSPFLSVAFYGTSAEGRPMPVVVASKEKAFTPEAAAR
                     SGKPVVLVLNGIHSGEVDGKDACLILLRDMALGNRPDLLETMTLLVVPIYNVDGHERS
                     SKWARPNQDGPVDGMGFRTTAQGLDLNRDFLKADAPETRALLSLVAAWDPDLFVDDHV
                     TDGSDAQVTVTVSYGGEPATPEPLRSWLETVMRPALAEVGENGWKTSPYIEWVDPLDP
                     HKGIDPGPTEPRYGTCYMPLRSIPSVLVEMHALKPYGERVRANAAFLSALLSRVGRDP
                     APLKAARAAAKAATKGARPGTPFVLAGETDLSRPETIDFPAYAWEQVVSPVTGRPRLV
                     YDPKTPMTIPLPFYRHAKATVTAPRPAAYLVPAGWPGIEEKLAAHGLRFTKLAAPRTL
                     PVGTYRASDAKFAPASYQGRVRVTARIARAVETRTVPAGSLYVPLDDPLAPVAIHLLE
                     PEGPDSLFAWGLMSSALETKEYIDARVLEPLAVKMLAEDPKLAAEWKALLDDPKRAAD
                     ARARHRFFYARTPYWDESVGLLPVYRLDAPLEPTPPAAPAP"
     gene            13718..14119
                     /locus_tag="P5164_04280"
     CDS             13718..14119
                     /locus_tag="P5164_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43145.1"
                     /translation="MKRTLVALAAAFALVVTTASVAAGNTAPRKGPSCCAEAGEAGAK
                     CEPVSESAVTLTGTVLCEHCDLHVAKSCNPVFKADGREGYLPFCPGTKDVDGVKAAAD
                     HGKVKLEVKGKLCKTKDGKELLMVETYAKKA"
     gene            14132..14536
                     /locus_tag="P5164_04285"
     CDS             14132..14536
                     /locus_tag="P5164_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRY43146.1"
                     /translation="MNAPIQRPWRTGRRLLAGLVLLLALPLAAGQASLNVYFQSTLKD
                     AGYQKKVFQKVAAAWKAPATFPKAGKKCVVQAVLSRDGRVQSAFVSTESGVKAWDKAS
                     LAAVESSSPFPPLPESYAHKTLEAHFHVAVVP"
     gene            14735..>15224
                     /locus_tag="P5164_04290"
     CDS             14735..>15224
                     /locus_tag="P5164_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HRY43147.1"
                     /translation="MSEKPFQPFVPASSTMTEFTGRALVLGLLMAVVLGAANAYLGLK
                     AGMTIAATYPAAVIGMAVLRIMKGSLLEENFARTVGSIGESVAAGAIFTIPAFVILGM
                     WKFEGFNAKEYGIATALMILGGLLGILFVTILRRVMVEDKGLPFPESVAASEIHKAGQ
                     RGA"
BASE COUNT         2153 a         5350 c         5471 g         2250 t
ORIGIN      
        1 ctcgcttgac acgagcattc aaggaggcgt agggtgccgg tgtatcggta cagcgatacg
       61 ctggccgaac cgaaaggaga gcccccatga aacgcctctc gtactgtctg ctctcggcgc
      121 ttctcatcgg tgccgctgcc gttccagttc aggccgtcac cccccgccag tgcgtccgta
      181 aggtcgcaga cagtaccctt tcctgcacct acacgctcat cgcgatgcgc tccctccaga
      241 ccgccgtcga ggcctacgct atcgacaagc atcagtatcc tgtcgctcag tccatcacgg
      301 aactcaagag ccttgtccag cccacgtaca tcgtgacgac cccactgacc gacaagtggg
      361 gcaccgagtt ccgctatgtt ctgtcagccg acgggaagag ctaccggatc gtgagtgctg
      421 gctccgacaa ggtctttgac gactcgtcgt gggggtctcc cggcctcctc gagtcctcga
      481 aggaagacgc ggtggtgtcc tccaacggtc gcgaccggga gtgggccatt caggagtagg
      541 tcgcgtgcgg gcggtgcccc gggaggcgcc acggggcacc gcaccgctca cccagctaac
      601 ccggcgtgcc gcggacttcg cttcgctcgc cgcggacgct acgatgaggc cgcgcgaatc
      661 ccatgcacat acctcagggt cagtccgtcg catatgagtc cggccagcga cacgaggtcg
      721 agctcgtgct tgcagcgctc cgcgacgccg ccattcccgc agtcgacgtc tactactccg
      781 acaccggcga agcgccaacg cccggccaga cctcgttcac cctctggctc atcctcgtgc
      841 cgacggaggt tcttcccgca gctcgttccg tcatcgcgcc tttgcccgtg tcccgcacgg
      901 acgcgccggc ggcacccgtc gcgccggcgt ccgtccgcgc cgccctggtc gtcctcgcga
      961 tcgtcgcggc tttcctgctt ctcctggtct ccttcctggg gggggcatga gtcccgcgtg
     1021 gcggcctctc ctcgtcgtca gcctcgcgtc actgctcagc gcggcctgcg cgcacgcccc
     1081 cgcggtggac ccgtcgtacg cgcgcccatg cccgtctccc gacggctgcg tcacgttccg
     1141 cgtcacgggt gccgagtctg cggatcagcc tcgcgacaca ccggtcttcc tcgtcgcagg
     1201 agcgagcgtg tcgtatttcg gttcgaccga ttccgacggc atcctcgtca tcccgaaggg
     1261 caccctcacg gtgcccggcc acctggtcct gctcttctgc tgggacagcc gaagcgaagc
     1321 ctgcacggcc gtccggctcg actccggcgc gatcgccact tacgactggg tcaacgtcag
     1381 cttgcccgtc aatcggctca ctcgaaggtc ccaggcgcat ccggcgcctc aggcaacccc
     1441 gctcccgtcc ccctagttct cgtcgcaggg tcgccgggcc ctgccgctgc cgacggactc
     1501 gcctcgctcg ccctcgaggc tgcgcctcgg cgcgcgcccg gacgagccgc gagaggagtc
     1561 atgacgacgc gcgcgactga tcagaccacc gccggcgtcc cgtttccgcc gcccctcgcc
     1621 ttcctcgcga cgctcgggct cggcttcgcc cttcatctcc tcttccctct tcacctgggc
     1681 cggaccaccg tgctcatcga agcgctggag cggacgggaa cggcgcttgc cctggcggct
     1741 ctcgtcctgg ccggctccgc gttcgcatcc ctccggcgcg ctcgaacgag ccctttcccc
     1801 gagcgtccca cgacggccct cgtggtcggc ggcccgttcc gattcacgcg caatcccctg
     1861 tacctcgcga tggcgctcct tcacgcgggc atcgcgacct tctcgaacgc cctcctgccg
     1921 ctcctgtgcc tcgtcccggc cctcgtcgcc attcgattcc tcgtcatccc tcgcgaggag
     1981 cgctacctcc ggcttcggtt cggcgccgag tacgaggcct actgccggtc ggtgcggcgt
     2041 tggctgtagc cgttcgggct catcacgggc gtccctgcgc cccccgcccg gcggccttcg
     2101 ctgcgttcat ctcggacgtc cccgttccgg cgccggaagg ccgtcctcga aggagccatt
     2161 catgctgagc cgcctcatac cgatgctgcc cgtccgacat gtgcccgcga gcgtcgcgtt
     2221 ctacgggaag ctcggcttca ccgtcgagac gaggcacgag gagtgggggt gggcgatgct
     2281 ccgcctcggc gaatgtcgcc tcatggtcga tcaatcgatc aacgtccatc cggacgctcc
     2341 tcgagatccc gtcctctacc tgtacccgga cgacatcctc gagtatcacg aggccgtccg
     2401 acgcagcggc ctcgacatcc cggacctgga ggtcacgttc tacggcatga cggagttccg
     2461 gatcgacgat ccggacggca accggctctg gatcgggcag aacgtctcgg gagcggagta
     2521 gggagccggc actccggtcg tcggagccgc gcgctttcca cctcgatccg gagtcccgaa
     2581 ggcggagagg aagagatgtc gctacggatt caggaggtcc ctgccgcaga ggcgccgctg
     2641 gagctgcttc tgctcgccga tccttcggag gagaagatcc gctcctacct tccggaatcg
     2701 aggtgcttcg tggcttcacg cggcgcggtc gtcgtgggcg cgtgcgtcgt gcagccgcga
     2761 ggtgcgggcg cacacgagct catgagcatc gccgtcctcc cggaacacca gaagtccgga
     2821 tacggaacgg cgcttctgag atgggtcgtc gacctctatc gccgtgccgg cgcgcggcag
     2881 ctcgaggtgg gcaccggcac gttcggctat cagctcgcgt tctaccagcg gcagggcttt
     2941 cgcgtgacga gcatcgaccg cgacttcttc gtcaggagct accccgagcc gatcttcgag
     3001 aacgggatac agctcttcga catgctgcgc ctcacgctga ggtaccccgg caacgtggcc
     3061 tgaggaatcg tccccaccgg ggatgcccga ggcccggtgg cgtcccgggg agaggacgcc
     3121 caccgcaggt gcagctcttg cttatttcgt ccaacgggag acgagagata tgagaaatcg
     3181 cctcagcatc gcgctcctgt ccgcggcagc cgtcgtcctg gcttcccagg gctgcgtctc
     3241 gacggtcttc cgcccgagcc ccgaccgcga catcgtcgcc tgcccggcgg tggagccgtc
     3301 gtccgttcgc gtgctctccc ggcctcccca ggagccgttc acggtcctcg gggaggtcga
     3361 ggcgtacgtc tcgggctatt actccgacga ggagatcgtg agccgcctca gggcgaaggc
     3421 cgcgaagaag ggggcgcacg ccatctactt cgcgcgagac gtctcgatgg agacggcaga
     3481 gaggtggacg acgggggagt acgacacgcc gctgaacgat cgaacctggc gcaagcggtc
     3541 ctccctcgtc ttcagggccg tcctgctgtc cgacgcgccc gagccgtgcc cgggcgtcgg
     3601 ccggccggct ccggttcgcg gcggcgcgac cacggccgcc ccggccgtcg cgccgcgctc
     3661 ctcgggcctc tgagcccggc cgaccgcaca ttgaggcagg cgaaagcatg atcatcacgg
     3721 agaccttcga gccccggagc cgacgggact ggcggaagtg gctgggccgc catcacgccg
     3781 agagcaaggc catctgggtc gtgttcctga agaagcacac ggggcgggcg gcgttcacgt
     3841 atcaggacgc cctcgaggag gcgctctgct tcggctggat cgacggggtc cggcagagga
     3901 tcgacgacct tcgattcgca cagaggttca caccgaggac gaagtcgtcc cgctggtcgg
     3961 agacgaacag ggcgctggcc gcggacctgg aggcccgggg cctgatgact cccgcgggcc
     4021 tcgcggcgcg gggctcggcg ccgcccgact cgcctgcggc ccgggagccg gcgtccggcg
     4081 tcccggagtg gctggtcgac gctctgcgga cgagcccgct cgcgtggaag agcttcaacg
     4141 ccctgccccc ctcgcaccag cggcgctatg tgggatggat ctcgtccgcg aagcgggacg
     4201 agacgcgaaa caagcgggtc gcggaggcca tcgccctgct cgaggagaac aagcggctcg
     4261 gcctcgggcc cggagaggtc cggaagtagg tcgcgcgtcg tcctatgatt cccggcagct
     4321 ctcggacacg gaggtcccca tgaacgctcg cgcggtcctg gcatcggctc tgatcgccgc
     4381 ggggctactc gcgacggcct gcagcgacac gcccaccgag ccctcctcgc cggtcgccaa
     4441 cggcaacctc cagatccagg tcactcagcc ctgcgctctg tccgggagcg tcacggtcgc
     4501 cgcgaacggc cggcagctcg gcacgctcgc cgtcccgggc gagagcacct tctcggtgcc
     4561 tgccggctcc tactccctgg ccttcgtccg cggccaggac gtcttcgccg gtcccggccc
     4621 ggtccaggtg ccggccggag gcaccgtcgt cgtgaccgac cccgcaggcg cctgcatggc
     4681 gccgaccggg gcgccctgat cggccgcgct cgcagcgtcg gccgcgaccg cgcggcatcc
     4741 cggcccgcgg ctccggaggc cgaagagctc ctctcgtacg cggcgccagg ccgcggcacg
     4801 cgggacagca cgcacgggcg cacggagaag ccgaggagca ccatgaagac gagcgaacgc
     4861 gaagagctcc tgcacgtcct gaagggacgc ttcgagaagc acgggaaccg tcatccgggg
     4921 ctggaatggg cgaaggtgct cgcccggctc gacggcaacc ttccggcgct ccggtcgctg
     4981 ctcgcgatgg agtcgtcggg aggggagccg gacgtcctcg cgcgcggcgg agagaggggc
     5041 ccgtacacgt tctacgactg ctcggcggag agcccggcgg ggcgaaggag cctctgcttc
     5101 gacggcgagg cgcgaagggc ccgcaaggag caccggccgg agagcagcgc cgtcgagatg
     5161 gccgcggcga tggggatcga gctgctcacg gaggaggagt accggaccct ccagggcgtc
     5221 ggcgagttcg atacgaagac ctcgagctgg atcgcgacgc cgcccgacgt ccgggccctc
     5281 ggcggggccc tcttcggcga ccgccgctac ggccgggtgt tcgcgtacca caacggggcg
     5341 tcgtcgtact acgccgcgcg gggcttccgc gggctcctcc gtgtctgagc gcgctggttc
     5401 ggggctcgac ggacgcatgc agatcctcgc cgtctcggga agcctccgcg aggcttcgtc
     5461 gaacacgacc ctcctccgcg ccctcgcgac gctcgcaccg gcggacaccg ccgtcggctt
     5521 tccggtgccg ctcgactcgc tgccgttctt cagccccgac gtggaggagg cgggccttcc
     5581 cccgtccgtt caggcgtggc gtgcggacgt cgcccgcgcc gacgcggtcg tcgtctccag
     5641 cccggagtac gcgcacggcg tgtccggcgt cctgaagaac gctctcgact ggctcgtcgg
     5701 cggcatcgag atgagcggca agccggtctc ggtgctcaac gcgcggccgc aggccacgat
     5761 cgcgcacggg gctctcgtcg agacgctgcg ggtgatgggc gcgcgcgtcg tggacgagac
     5821 cgccgtacct ctcgcgggcc gcaagctcga ccacgcgggc atcgccgccg accccgagct
     5881 ggccgcaatg cttcggaccg tcctctccac tcttcggaag gcggccgaag accgcgacgc
     5941 cggcccggcc tgaccgcggc gtcgagcggc ctccaccccg ggtaggagcg cgccgcgccc
     6001 gcccgtcgtc cgacggcgcc gcgcatagac tcgcgcctcg atgtcgaccg atccgctcgc
     6061 ttcgcgcctc gcgttcctgc gagaggtcga cgggctgaag tccgtcgtcc gccagtctcc
     6121 gctcctcgac ggaagccgca gggagaacag cgcggagcat tcctggcatc tcgccctgta
     6181 cgcgctcgtc ctgcacgacc tcgcggcggg tccggtcgac gcgtcccgcg tgatccggat
     6241 gctcctcgtt cacgacatcg tcgaggtgga cgccggcgac acgcccctcc acgccggcgt
     6301 gcccccgcgg gagcaggccg agcgggagga ggcggccgcc gagaggatct tcgggatcct
     6361 cccgcggcgg gagggagagg agctcctcgc gctctggcgg gagttcgagg ctgccgagac
     6421 cgccgatgcc gcgttcgcga aggcgctcga cagggtccag ccgctgatcg cgaacgtcgc
     6481 cacgggcgga gggacctggc tcgcgagccg gctcacgcgg gaccaggtgc tcgcccgcta
     6541 cggaccgacg atcgagaagg gctcccctcg gctctgggcc gcctgcgagg cgctcgtgag
     6601 gagccacttc tccgggagac cggacgcttc ggacgagacc ggtgccgggg ccggggccga
     6661 cccgtccgcg accgacgcac gagatcacga agggggatga cgatggtccc gaagaacacg
     6721 atctgcctct ggtacgacgg caccgccctg gaagccgcgc agttctacgc ccggacgttc
     6781 ccggacagcg cggtcgggaa ggtctaccgc gcgcccgccg actacccctc gggcaaggag
     6841 ggcgacgtcc tgaccgtcga gttcaccgtg atcggcatcc cctgtctcgg cctgaacggc
     6901 ggcccggcgt tccggcccag cgaggcgttc tcgttccagg tggcgacgga cgaccaggcc
     6961 gagaccgacc gcctgtggga cgcgatcgtc gacaacggcg gccaggagag cgcctgcggc
     7021 tggtgcaagg acaggtgggg gctctcgtgg cagatcacgc cgcgcgcgct caccgccgcg
     7081 atcgcggacc ccgatcccgc cgtcgcccgg cgcgcgttcg aggcgatgat gcagatgaag
     7141 aagatcgaca tcgcggccat cgaggcggcc cggcgcggct gacgcgcgat ggcgtccggc
     7201 cgcggccgcc tctcgaccgg taccattcgc tcatgacgtt cgagctgcag ccgacgctgc
     7261 ggggcagcct cctcgagctg cgtccccctc cgcacggagg actgggcaga cctgttcgcg
     7321 gccgcgtccg atccgcggat ctgggagcag cacccggagt cgacccgcca cgaggaggag
     7381 gtcttccggc gcttcttcgc gggggcgctc gaatcgggcg gcctggctcg ccgcctgaag
     7441 cgaacctggc gtgcctcgcc tctcgtttcc gccgttccgg ccacgtcgta cgatgggaga
     7501 cgcatgatct cgagaagcct cctccacgcg tacggtttcg tcgcgccgct cgccctgccg
     7561 cgggcctgac gcggcgtgcg gcagccatcg atctccccgc ggggcgcggg gcttcgcggg
     7621 gaacgtgcag acgccgggag ggcgagatga cggtcgagaa cgaacgcgac gtggagggga
     7681 tcctcaaggc cgggcgcgtc gtcgcgacgg tgcgggacgc catgctcgag aaggtcgagc
     7741 ccgggatgac gaccgccgag ctcgacgaga tcggcggccg cctgctggag cggcacggcg
     7801 cgaggtccgc gccccgggtg acgtacggct tcccgggctc gacctgcatc agcgtgaacg
     7861 aggaggcggc gcacgggatc cccggtcccc gggtcatccg ggccggcgac gtcgtgaacg
     7921 tcgacgtctc ggccgagctg gagggctact tcgccgacac ggggggcacg accgtcgtcc
     7981 cgccggtctc ggacgcgaac ggctccctct gccacgcgac gcgggtggcc ctcgacagcg
     8041 cgctctccga ggcgcgcgcc ggcgccccgc tcaatcggat cgggcgagcc gtcgagcggg
     8101 tcgcccgggc gcacgggttc cgggtgatcc ggaacctcgc cggacacggg gtgggccgga
     8161 agctgcacga ggagccggaa gggatcgtga gctactacga ccggggcgac ggccgccggc
     8221 tgcggttcgg ccaggtgatc gccatcgagc cgttcctctc gacgaggagc gcccacgtga
     8281 cggaagcgga cgacgggtgg acgctcgtcg ggcacccgga gaaccgctcg gcgcagttcg
     8341 agcacacgat catcgtcacg aacggcgagc ccatcgtcgc gacccgctcg gagagaagcg
     8401 gctgacggga cggcgcgctc catcgcacgg cgcgccccat ctggtgcggg cgatgccgga
     8461 gcggccgcgc gcggcccgcg cgcggggcac ggcgtgcggg cccgatggcc ccagatccgg
     8521 atcggagctc gcggcatgag cacggttcgc atcgccctcg cgaacgtccg tgtgcccgcg
     8581 acgccgaatg actcggtgca cctggcgatc gcggcggtcg ccgaggcggg ccgacggggc
     8641 gcgctcgtga tctgccacga agggcggcgg catccggagg ccgtccgctg ggccgtgcgc
     8701 gtcgtcgcgc gatcgaatcg actccgcaga cgggaggtct tcgtgagata tctgctcggt
     8761 gtcggcggcg tgatgctcct tcaggcgggg ctctctttcg cgatcatccg ggccacggcc
     8821 ggcggggggt ccttcgtcgg tctgggcgtg atgctggcgg cggtcccggg catcccgctc
     8881 acggccctgg tcaatgctct cctcgttcgc tcgagtcgga aggacccggc cgcggcctgt
     8941 gcccggcgtg tcgtcctcgc ctcgctggcc cttccggccg tgcagctggc cctcctggcc
     9001 ctcgtctccg cctttcggct ctgagcgtcg attccggtcg gcgtgcgcgc cccacctggt
     9061 gcgggcgtca cggcccgcgc cccgaacgct tcgaccggcc gcgtgacggg agacctgctc
     9121 gagagattcg gcggcgggga cggcgggctg agatccggcg acgcgccgag ctcgtcgcga
     9181 gaagcacggc ccggccgtcc cgggtcgagg aggacgccat gagagcttcg gtcttcgtcg
     9241 gcacgagcgt cgacgggttc ctcgctcgcg ccgacggcac gttcgacttc ctttcggccg
     9301 gcggctcggg cgaggagccg cacggtttcg aggagttctt cgccacggtc gacgcgctcg
     9361 tgatgggccg gagcacctac gaggtcgtcc tgcccttccc cgaatggccc tacggggcga
     9421 agccggtctt cgtcctgagc catcggccgc tccctccgga accggccgga gccgtcgtcg
     9481 agcggatggc cggcgagccg gccgaggtct tctcgcagct cgcggcgcgc gggttcgggc
     9541 acgtctacgt ggacggcggc cggacgatcc aggcgttcct gcgggccggc ctcgtagagc
     9601 gcctcgtcat ctcccgtgtc ccggtcctcg tcggcgaggg catcccgctc ttcggcccgc
     9661 tcgacgccga cgtgctcctg acgcacgtcg cgacgcgcac gtttgccggc ggcctcgtcc
     9721 agagcgagta cgaggtcgca cggtcgccgg aggcggaccc gcaggccgcg ccgacgcgct
     9781 actctcccgg gcacccgcca tgaacgtcgt caacctgaaa gagcgcctct cccggttcgc
     9841 cacctactgg gacccgaaga tcgtcggcga gctgaacggc cagcacgtga agctggcgaa
     9901 gatgaagggg ccgttcgtct ggcacagcca cgaggccgag gacgagctgt tcctcgtcct
     9961 cgacggctcg ctcacgatgg agttccgcga ccgcaccgtc cccctccgca agggggagct
    10021 gctgatcgtc ccccgcggcg tcgagcatcg cccggtcgcg gaggaggagg tccacgtcct
    10081 ccttttcgag ccggcctcga cggtcaacac agggagcgcg ggcggtccgc tcaccgtggc
    10141 ggagccggag cgcctttgac gcgagtcgcc cgcatcggtt ctgccgagcc gcgggacgtt
    10201 tcgggatgac tggccctcgc gaagggagtg gcttcggcgt ggagctgagg aacgtctgcg
    10261 tctactgcgg atcgagcgcc gggcgagacc cggcgtacgt ggcggcggcg ggggcgctcg
    10321 ggcgggcgct cgtctcccgc gggatcggtc tcgtctacgg cggggcgagc gtcggggtga
    10381 tgggggcggt ggccgacgcg gtcctcggcc tcggcggccg cgcggtcgga gtgatcccgg
    10441 aggcgctcgt ccggaaggag atcgcgcacg gcgggctgac ggagctcgtg gtcacctcct
    10501 cgatgcacga gcggaagacg cggatggcgg agctctccga cgccttcgtg gcgctgccgg
    10561 gagggatcgg gacgctcgag gagatcttcg aggcgtggac gtgggggcag ctcggcctcc
    10621 acgccaagcc gttcgggttc ctgaacgtgt cgggctactg ggacggcctc gtctcgttcc
    10681 tcgaccacgc ggtgcgcgag cggttcgtga gggagccgca ccgcgcgatg ctcgtcgtcg
    10741 ccgaggaccc ggaggagctg ctcgaccgct tcgcggcctg gcgggcgccc gaggtcccga
    10801 agtggatccg aaagggcgac gagtagggcg ctccggcgag ccgctcgcta tacttccggc
    10861 aaagctcgac ggacatcccc ccggacgcgg aacggcgaga gccgggaccg ccagaggctc
    10921 gtcggcgcgc gccgcggtcc ggggcgagga cgaaggaggc gccatggcac tgcacgcggc
    10981 gattcaggct ctgcttggag cagccggggt gacgaacggg atgcccggct gggcgatcct
    11041 cgagagcgcc cggctgctgc cgcccacgcc cggctccggg gcggcgttcg gggacgcgcc
    11101 ggcgcaggcg ctccggaaga cgatgaaggc gtactacgag tacggccgga accactggac
    11161 ctgggcgcag agcgcgggaa gcgcggccgc gaacggaggc ctcgtgaagg ggcagatcca
    11221 ggcggtggcc tgcgggtcgt tcaaccagaa cttcaagtgg ctggccgaga aggggctcgg
    11281 catcaccggc atcacgaacg gtcaggagac cgggcagttc ctgacgatgc cggggagcgt
    11341 ctgcatcgac tcgaaatggg tggggaacgt ccgcacgagc acgaaggcgt tctcgacgct
    11401 gaagtgcttc aagttctccg gccactactg ggtgatgcac ggcggcgtga actacgacgt
    11461 ctgctacgac aacacgttcc acgcggtctc cgagatcatc tggacgcgcc tgctgccgcc
    11521 cgaccagagc ctcgtcggga agggggggct cacggcgaac cagctctacc gcctggagaa
    11581 gccgctgccg gagggcgacc acctcgtgat gctccagcag aacgggccga acggctggcc
    11641 ctcctggcag atcgtcccga aggcgcagat caagtcgctg aagtaggcgg ccgcggggag
    11701 cgggcgctcg gatcggagcg cccgttccct cgcgggcagc ggcgagaggc gccgtctcta
    11761 cggcgccggg gctgcgggcg gcgtcggctc gagcggcgcg tcgaggcggt agacgggaag
    11821 gagcccgacc gactcgtccc agtacggcgt ccgcgcgtag aagaagcggt gccgcgcgcg
    11881 ggcgtcggcg gcgcgcttcg ggtcgtcgag gagcgccttc cactcggcgg cgagcttcgg
    11941 gtcctccgcg agcatcttca cggcgagcgg ctcgaggacg cgcgcgtcga tgtactcctt
    12001 cgtctcgagg gccgagctca tcaggcccca ggcgaagagc gagtcgggcc cttccggctc
    12061 gaggaggtgg atcgcgaccg gcgcgagcgg gtcgtcgagc gggacgtaga gcgagccggc
    12121 cgggacggtg cgggtctcga cggcgcgggc gatcctcgcg gtaacccgga cgcgcccctg
    12181 gtaggaggcg ggcgcgaact tcgcgtcgct cgcgcggtac gtgccgaccg ggagcgtccg
    12241 cggcgcggcg agcttcgtga agcgaagccc gtgcgccgcg agcttctcct cgattcccgg
    12301 ccagccggcc gggacgaggt aggccgccgg gcgcggggcg gtgacggtgg ccttcgcgtg
    12361 gcggtagaag ggcagaggga tcgtcatcgg cgtcttcggg tcgtagacga ggcgcggccg
    12421 gccggtgacg ggggagacga cctgctccca ggcgtaggcg gggaagtcga tcgtctcggg
    12481 gcgggagagg tcggtctcgc ccgcgaggac gaagggcgtc cccggtcgcg cacccttcgt
    12541 cgctgccttc gcggccgcgc gggccgcctt cagcggcgcc gggtcgcgcc cgacccgcga
    12601 gaggagcgcg gagaggaagg cggcgttggc tcggacgcgc tcgccgtacg gcttgagcgc
    12661 gtgcatctcg acgaggaccg aggggatcga ccggagcggc atgtagcagg tgccgtagcg
    12721 cggctcggtc ggcccggggt cgatcccctt gtgcgggtcg agcgggtcga cccactcgat
    12781 gtagggcgag gtcttccacc cgttttcccc gacctcggcg agggccggcc gcatgacggt
    12841 ctcgagccag gagcggagcg gctcgggcgt cgccggctcg ccgccgtacg agacggtgac
    12901 ggtgacctgg gcgtcggagc cgtcggtgac gtggtcgtcg acgaagaggt cggggtccca
    12961 ggcggcgacg agcgagagga gcgcgcgcgt ctccggggcg tcggccttca ggaagtcgcg
    13021 gttcaggtcg aggccctggg cggtcgtccg gaagcccatc ccgtccacgg ggccgtcctg
    13081 gttcgggcgg gcccacttcg aggagcgctc gtggccgtcg acgttgtaga tcgggacgac
    13141 gaggagcgtc atcgtctcga ggaggtcggg gcggttcccg agcgccatgt cgcgcaggag
    13201 gatgagacag gcgtccttgc cgtcgacctc gcccgagtgg atgccgttga ggaccaggac
    13261 gaccggcttg cccgacctcg cggccgcctc gggggtgaac gccttctcct tcgaggcgac
    13321 gacgaccggc atcggccgcc cctctgcgga ggtgccgtag aaggcgaccg agaggaaggg
    13381 cgaggtcttc tccagccgcc ggaggaaggc gagcgtctcg tcgtacgacg gcgtcccgcg
    13441 gaagttgttc agctcggcct tcgtccagtg gtcgggctgg atcggaacga gcggccgctc
    13501 ctcggccgcg agcggagcgg cgaggacgag agcgagggcc gcgagaagcg cggagcgggc
    13561 gttgaggcgt gccggacgca ttcgaggagg ttatcgcggg cggcctcgac gcgcgcctcg
    13621 aggtgtcggg gcgcccggca tcccgacgac acggaggtat tcttctctcc cggcgcccca
    13681 cccgggcgcg accgcacccg aaggaggacg tcatcccatg aagcgcaccc tcgtcgccct
    13741 cgccgccgcc ttcgccctcg tcgtcaccac ggcctccgtc gccgccggca ataccgcccc
    13801 gaggaagggg ccgtcctgct gcgccgaggc gggggaggcc ggagcgaagt gcgagcccgt
    13861 gtccgagagc gccgtgacgc tgacgggcac ggtcctctgc gagcactgcg acctgcacgt
    13921 cgccaagagc tgcaacccgg tcttcaaggc ggacggccgg gagggctacc tgccgttctg
    13981 ccccggcacg aaggacgtcg acggcgtgaa ggccgcggcc gaccacggca aggtgaagct
    14041 cgaggtaaag gggaagctct gcaagacgaa ggacggcaag gagctcctca tggtcgagac
    14101 ctacgcgaag aaggcgtagc cggagcggcc ggtgaacgca ccgatccaga ggccgtggcg
    14161 gacggggagg cggcttctcg ccggcctcgt cctcctcctc gccctgccgc tcgccgcggg
    14221 gcaggcctcc ctgaacgtct acttccagtc gacgctgaag gacgcgggct accagaagaa
    14281 ggtcttccag aaggttgccg cggcctggaa ggcgcccgcg acgttcccga aggcggggaa
    14341 gaagtgcgtc gtccaggcgg tcctcagccg ggacggccgg gtccagagcg cgttcgtctc
    14401 gacggagtcg ggcgtgaagg cgtgggacaa ggcctctctc gccgccgtcg agagctcctc
    14461 tccgttcccg cccctgccgg agtcgtacgc gcacaagacg ctcgaggcgc acttccacgt
    14521 cgccgtcgtc ccgtagcgga ccgggcgccg aacggcggga ctccgcggcc gtgccgcccc
    14581 gcggcaaggc gaaacgcccc gcctccgggc agcgaaagga cgggccttcg gggcgcggtc
    14641 gacgccgcag tcgcatgtag aatccgcgtt tgatgcgcct ccggcctcac agccgtatcc
    14701 atcgagatca gatcgcgagg gaggatcgtc cccgatgagt gagaaaccgt tccagccgtt
    14761 cgtcccggcg agctccacca tgacggagtt cacgggccgg gcgctcgtcc tcggcctcct
    14821 gatggccgtc gtcctgggcg ccgcgaacgc ctacctcggc ctgaaggccg ggatgacgat
    14881 cgccgcgacg tacccggccg cggtgatcgg catggccgtc ctccggatca tgaagggctc
    14941 cctcctcgag gagaacttcg cccgaaccgt cggctcgatc ggcgagtcgg tcgcggcggg
    15001 cgcgatcttc acgatcccgg cgttcgtcat cctcgggatg tggaagttcg aagggttcaa
    15061 cgcgaaggag tacgggatcg cgacggccct gatgatcctg ggcggcctcc tcgggatcct
    15121 cttcgtgacg atcctccggc gcgtcatggt cgaggacaag gggctgccgt tccccgagtc
    15181 ggtcgccgcg agcgagatcc acaaggcggg tcagcgcggg gccg
//
LOCUS       DAOTEZ010000052        15111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2699_length_15111_cov_6.418371, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000052 DAOTEZ010000000
VERSION     DAOTEZ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15111
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2699_length_15111_cov_6.418371"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2135
                     /locus_tag="P5164_04295"
     CDS             <1..2135
                     /locus_tag="P5164_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43148.1"
                     /translation="LLPSIQWMEEIQVAGLGANAEYGAYTGAVVNGVTKSGGNELKGT
                     LEAYYQPDSFVSDNSPDTADETFTFEDYALSLGGPILKDKLWFFGSVEYWHQETTPVG
                     AMDTSDRKIPRFLGKLTWQLNEKNRIFAMGEYDKVTNERRGIDAFTFPEATSKQDGPN
                     VTFGANWESILSSSNFLNVKVTGYDGNDDFLPYAGHDVAGHEDYYGYSGFAVVNQKIQ
                     ELNHRHKMSGDVNWSLFKDGLFGANDSHSFKIGVLYERSSTTDNWRRNGGFTYYDDST
                     LCPGATEAEQIANYLKDPTCGWYERSTGYGEYDVNAETEGFTAYAQDSMRIDRFTVNY
                     GLRYTSYTGGWRSGFGNPDVYKADFVDPRIGFAWDVLGNAKLAVKAHWGRYHSSMYTY
                     LFDREASGEGSIPDGDCYWDGSAWGDCSSSVPDYATMGNIDHPYVDETLLTIEHQLGK
                     DMAVGADYINRRFRSLLGMVNTNDDYERVTGIPNPIGGGELEVWSLNGDPVWSLTTDN
                     PGERDYDSFVVRFDKRYSHGWQLRSSLVWTDLKGNVLKNNGYAPELEDRNGLVNAYGK
                     MDSSFNEWEFKVSGSVDLPLGFAASGQYTYLSGTYWTPNGSVRRYLDGNSASGRYIFL
                     TERGSQQLEDRNILDLRLAWGTKIAGESRLDLSLECFNVLNTGETLTIDDYYGEYRSG
                     TWRKSATYGATLSIETPRQLRLGARFSF"
     gene            2252..3127
                     /locus_tag="P5164_04300"
     CDS             2252..3127
                     /locus_tag="P5164_04300"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HRY43149.1"
                     /translation="MRSSRALPLLFAFSLLLLGAKAPFALGAEVPARGHLVLIGGGDK
                     PPEVMRKFVELAGGKDAPIVVIPTASSEADAPDYYEKLFREELGCLDAVSLRIRSRAD
                     ASRPDFVALARKARGVFFGGGDQIRITNALLGTPVGDAIAAAFAGGAVVGGTSAGTAC
                     QSEKMITGEGDFTQVRTRSVELWQGLGFLPPDVVVDQHFLRRQRQNRLLSVVLENPSV
                     LGIGVDEETAIWVRPDGTLQVLGESGVMVFDAKGAALSRQPRPTGQDLLGVRGLSLHL
                     LLPGETFNLSSRRPL"
     gene            3319..4572
                     /locus_tag="P5164_04305"
     CDS             3319..4572
                     /locus_tag="P5164_04305"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRY43150.1"
                     /translation="MYERRVRPAEAAEKVDWEARVTALLREEPGDLVVTAVGDVIFNE
                     RISHLEAPDRKNLLRILQEADVAIGNLEFSINDRPELQKVFYNFRAPREFAWELAATG
                     ISLVSLANNHALDFGREGLLECLRALDLSGIAHAGAGRTLAEAREPGTKRPPGQKGRV
                     ALLSYMRYWTDRDRCADPSGPCLATIDPATVLVARSDGTVESVEGPQERDVKAMEEDV
                     LLAKRRHGPVFVAYHVHDVSHARVFGIQDTTPPNEEIPFRRAIDAGADAVLGSGPHVL
                     RGIELRNGRPIFYSLSNFIYQYRTPKAIPTDLPHQRDSEMPRPANVSVWDRRDSREVM
                     ESVVARMTYRDGLLRRLELIPVTIDDEGPLFGVPRLASTKRAKEIVELLQRLSAPYGT
                     KIAWKGWYGEVELPAIASPSPQTAS"
     gene            4660..4821
                     /locus_tag="P5164_04310"
     CDS             4660..4821
                     /locus_tag="P5164_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017956220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43151.1"
                     /translation="MCHPVRCPKCGKPTWAGCGRHVEEALAGVPVEKRCSCPPPDSLL
                     SRLFGSRKS"
     gene            complement(4852..6528)
                     /locus_tag="P5164_04315"
     CDS             complement(4852..6528)
                     /locus_tag="P5164_04315"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRY43152.1"
                     /translation="MRKTAFVPALAIGAVFGGVLAAPPAVAQPSPASAAPATSAADAA
                     RPRFVLPKAGEAIPAPLRAALDGVRADALRAHVSFLASTPLAGRGLGSDGLEAALEYG
                     AAQLALVGVAPYGAADGKGARTFFQPVPMREIREPGGSVEIERRTGEATARRTFVHGV
                     DVVVPPQPARSVSAPLVFAGYGLREASPARDDYRGLDVKGKIVVLREGLPPGEAWSSK
                     ELVEKYAAGDSEERWAAKLETATALGAVAVLAVEGNDWAARLLGNEKPATFAFRSMTE
                     APAREPLVVRCAPVVLEALLGAPPKVEEPSRPLPASATLRATALERTGLSRNVVGILP
                     GSDPARADEAVVLGAHADHLGRVDGVIHPGADDNASGIAALLEIARAFASSPVRPKRT
                     LVFALWTGEEEDKIGSGHWVRSPLWPLAKTAVYLNFDMIAHPWKAEEIRDLVASAKLP
                     GAEAFLAAARPDTFLEPGVASFAPDLGPLLARAGGAADLALHLDWTEGVNGGSDYRDF
                     ARARVPFVRFFGNFHPDYHRPGDTPASIDPDQVRRVTRLGLATAWLAADR"
     gene            complement(6525..7679)
                     /gene="menC"
                     /locus_tag="P5164_04320"
     CDS             complement(6525..7679)
                     /gene="menC"
                     /locus_tag="P5164_04320"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015374231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HRY43153.1"
                     /translation="MSGPAERASALPLEAERVPAVERVDLVHVSLPFVKPFSISTATW
                     TGKETFLVRVEGGGKVGWGECVADPDPFYAPETTTSARHVVAEFLLPLVEEGRTFLEL
                     DERFGHVRGNRMAKAALENALVDLAAKAKGVPLHELLGGPRKRVPSGISIGLQDSPAA
                     LVREVEEAVARGYHRVKVKVKKGKDVEYVAAVRERFPDLPLMADANGDYRLADAAHLA
                     RLDAFGLTMIEQPLSYADIFEHSLLQRELATPLCLDESIHDLADFRAAVELGACRVLN
                     LKEGRVGGLLESLRLLAFARERGIPVWSGGMDETGIGRAVNIHLQTVEGFTLPGDTSE
                     TSRYFREDLVTPSVVLDAEGFVEVPAGAGIGVDVVPERLEKYTLRTERLK"
     gene            complement(7676..9193)
                     /locus_tag="P5164_04325"
     CDS             complement(7676..9193)
                     /locus_tag="P5164_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HRY43154.1"
                     /translation="MKAFPLLLAALLAPVPGAAVPGVASGALPEDHRTLTKSTSYDAM
                     AAFLKTIDGKGPVVVSVEGQTAQGRSIFLVNARGSAASTFRVLFYAQQHGDEVSGKDA
                     LLYLLRDLARDPKLLPPEVDLWVMPMVNPDGAEAGTRRNAAAADLNRDHVTLFQPETE
                     ALHRVARRVRPHLAVDAHEFGRDSEEWTRKGWEKWPDITMDGLNNPHFDRERVAAAKR
                     WVDASAAPLEKAGHRFLRYWVGGAPPDDEQRHSAPDTDGGLNGVGAYGGLSFIIEAAV
                     RHLAPDPQADLGRRVDAYLVLFRRFLEGGGHRDEDRAVVEKARKLPLPAFLPSDALWV
                     NPEGTVTEFPVVELATGKTIRVPTANVMTDVAVKRSVPTPLGYAVEPAAAAEIGALLT
                     RHGLGFERIAAPRTVTAETCALLRVEEEFDDLYGRYGGRTIVKRGAARPVELPAGSLW
                     VPLEGPDALRAAILLEPTAMYGLWQYPRLKALVAWDGTIPVRRVVAMAATAGSPK"
     gene            complement(9204..10619)
                     /locus_tag="P5164_04330"
     CDS             complement(9204..10619)
                     /locus_tag="P5164_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43155.1"
                     /translation="MAEAASKSGRTFRMPHTLVIVGSLVVFVLVLAWLVPSGTYQTVE
                     KAGRTVTVPGTYQQVEKVWLGPQWVVVAPIRGFLDGALIIAFLFAIGGAFGIVQAGGT
                     IEFAIRKLTAALAARPFLEKLVIPVLMAVFSLAGSVFGMSEEVIPFVVICIPLAISLG
                     YDSIVGVAIPFLGAAAGFGAAFFNPFTVGIAQGLVGLPLYSGLGYRVATWFVTTGVMI
                     AWVMVYAARVKKRPEASPVRDLDLARERSAPASGEAEPWTVRHALVLSLFVTTMGVLV
                     WGILKKQWFIEEIGALFLAMGLVLGVVAGLGGTRIATAFVAGAKDMVNVALIIACGRA
                     LLIIAREAKVLDTILFHGAGLISHLPGVVAAQVMFVVQCVINFFIHSGTAQAALTMPI
                     MAPLADLVGLTRQTMVFAYQLCELINPILPTSAVTMGVLGMAKIPWEKWARWFLPLML
                     TLMVLAFLLLVPPVLMHWGPF"
     gene            10913..12325
                     /gene="hydG"
                     /locus_tag="P5164_04335"
     CDS             10913..12325
                     /gene="hydG"
                     /locus_tag="P5164_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014413849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HRY43156.1"
                     /translation="MTNSAFSPEPVVRIDDDGIWGDLESASRPDAGEVRDLLAKSREL
                     KGLRGRDLAVLANVADPALLDELFHTAREVKEAIYGKRLVLFAPLYISNLCANECTYC
                     AFRARNTALVRRALTQEEIAREVKWLEEQGHKRILLVAGESYPREGFDYVLRSIETVY
                     ATSSGPGEIRRVNVNVAPLTDEEFRRLKAAKIGTYQLFQETYHRETYAEVHVAGKKRD
                     YDWRVTAMDRAMRAGIDDVGIGILFGLYDWRYELLALMQHVEHLERTFGVGPHTISVP
                     RIEPATGSEVADHPPHAVSDLDFKKVIAILRLAVPYTGLILSTRESAETRREVLALGI
                     SQISAGSRTNPGGYEADPGDPCAEQFSLGDHRSLDEVIRDVASHGFVPSFCTACYRLG
                     RTGADFMDLAKPGEIKEHCNPNALATFQEYLLDYGSEETRRAGEARIAASLDEMEPGL
                     RERSRSMVEAVKSGRRDVFC"
     gene            12339..13595
                     /gene="hydF"
                     /locus_tag="P5164_04340"
     CDS             12339..13595
                     /gene="hydF"
                     /locus_tag="P5164_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HRY43157.1"
                     /translation="MSSAPRGLRTHIGLFGRRNVGKSSVLNALTRQEVAIVSATPGTT
                     TDPVEKPMELLPLGPVLFIDTAGVDDEGALGEQRIAKTRAALERTDLALLVAEAPEWG
                     PFEEELFGEIRARSIPVVVVLNKADTGPAPAGLAGRLAAAGVPAVATSIPSGTGLDEL
                     RQALLAAAPADAVEGRRIVGDLVSPGDVVVLVTPIDAEAPKGRLILPQVQTLRDLLDN
                     GAISVVVREHELHGALDALGRPPRLVVTDSQAFRQVAAEVPPGVPLTSFSILFARFQG
                     DLAEMARGVRGVDRLKAGDRVLVAEACTHHPVGEDIGRVKIPAWLRARTGVPLEFETV
                     QGRDFPDDLSRYALVVHCGSCMGNRRQMLSRLLRGRREGVPVTNYGLTIAYSLGILER
                     ALEPFPAALEAFRLAAPPPSSPEVTT"
     gene            13595..14659
                     /gene="hydE"
                     /locus_tag="P5164_04345"
     CDS             13595..14659
                     /gene="hydE"
                     /locus_tag="P5164_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HRY43158.1"
                     /translation="MDRDGILAWLRETDPTRLSRLYRRADAVRREHVGNEVHLRGLVE
                     VSNHCVRRCAYCGIAATNDGIERYRMTEEEVLACARQAVELGYGTLVLQAGEDYGLTR
                     EGVSSMVRAIKRETGLAVTLSLGERPEEDLVAWREAGADRYLLRFETSDAALYQAIHP
                     AMPGRPDRFAILGRLAVLGYEVGSGVLVGIPGQTWESLANDLLVFRQLDLEMIGVGPY
                     IPHPATPLGSGRFPVAPEGEQVLADEETTTKVVALTRLVRPDANIPSTTALATLDPSW
                     GRERGLLRGANVVMPNLTPPAYRQLYEIYPGKACVNETAEACGSCLPNRIRMIGRVPG
                     KGPGGRRRTVASGALSSVLG"
     gene            14784..>15111
                     /locus_tag="P5164_04350"
     CDS             14784..>15111
                     /locus_tag="P5164_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43159.1"
                     /translation="MGKWTGRSVVRVAALAGALMVPHSLSADLEDDLERAWVGAWVVT
                     KVETASDCSGTYTNVEVRGLRSTSRGSRRFGEGELARIDKLNQKSDRVDLYLSVAEPI
                     LVPRTDG"
BASE COUNT         2266 a         5609 c         5161 g         2075 t
ORIGIN      
        1 ggctcctccc gagcatccag tggatggagg agatccaggt ggccggcctc ggcgccaacg
       61 ccgagtacgg cgcctacacc ggcgcggtcg tcaacggcgt cacgaagtcg ggcggcaacg
      121 agctcaaggg gacgctcgag gcctactacc agcccgactc gttcgtctcc gacaactcgc
      181 ccgacaccgc ggatgagacc ttcacgttcg aggactacgc cctcagcctc ggcggcccga
      241 tcctgaagga caagctctgg ttcttcggca gcgtcgagta ctggcaccag gagacgacgc
      301 ccgtcggggc gatggacacg tcggaccgca agatcccgcg cttcctcggg aagctcacct
      361 ggcagctgaa cgagaagaac cggatcttcg ccatgggcga gtacgacaag gtcaccaacg
      421 agcggcgcgg gatcgacgcc ttcaccttcc ccgaggcgac gagcaagcag gacgggccga
      481 acgtgacgtt cggcgcgaac tgggagtcga tcctcagctc ctcgaacttc ctcaacgtga
      541 aggtgaccgg ctacgacggc aacgacgact tcctccccta cgccgggcac gacgtcgccg
      601 gtcacgagga ctactacggg tattcgggct tcgccgtcgt gaaccagaag atccaggagc
      661 tcaaccaccg gcacaagatg tccggcgacg tcaactggag cctcttcaag gacggcctct
      721 tcggggcgaa cgactcgcac tccttcaaga tcggggtcct ctacgagagg tcctccacca
      781 ccgacaactg gcgccggaac ggcggcttca cctactacga cgactcgacg ctctgccccg
      841 gggccaccga ggcggagcag atcgccaact acctgaagga cccgacctgc ggctggtacg
      901 agcgctccac ggggtacggc gagtacgacg tgaacgcgga gaccgagggc ttcaccgcct
      961 acgcccagga ctcgatgagg atcgaccgct tcacggtcaa ctacggcctc cgctacacga
     1021 gctacacggg cggctggcgg agcgggttcg gcaaccccga cgtctacaag gccgacttcg
     1081 tcgacccgcg catcggcttc gcctgggacg tcctcgggaa cgccaagctc gccgtcaagg
     1141 cccactgggg ccgctatcac tcctcgatgt acacctacct cttcgaccgc gaggcctcgg
     1201 gagaggggtc gatcccggac ggcgactgct actgggacgg cagcgcctgg ggcgactgca
     1261 gctcgtccgt gccggattac gcgacgatgg ggaacatcga ccacccgtac gtcgacgaga
     1321 cgctcctcac gatcgagcac cagctcggca aggacatggc ggtcggcgcc gactacatca
     1381 accggcgttt ccgcagcctc ctggggatgg tcaacacgaa cgacgactac gagcgcgtga
     1441 ccggcatccc gaacccgatc ggcggcggcg agctcgaggt ctggtccctc aacggggacc
     1501 ccgtctggtc gctcacgacg gacaacccgg gcgagcggga ctacgactcc ttcgtcgtcc
     1561 ggttcgacaa gcgctacagc cacggctggc agctccgctc ctcgctcgtc tggaccgacc
     1621 tgaaggggaa cgtcctcaag aacaacggct acgcccccga gctcgaggac cggaacggcc
     1681 tcgtgaacgc ctacgggaag atggactcct cgttcaacga gtgggagttc aaggtctccg
     1741 gttccgtcga cctcccgctc ggcttcgccg cgagcggcca gtacacctac ctgtcgggca
     1801 cctactggac ccccaacggg agcgtccgcc gctacctcga cggcaactcc gcgtcgggcc
     1861 gctacatctt cctgaccgag cgcggttcgc agcagctcga ggaccggaac atcctcgatc
     1921 tgcgcctcgc ctggggcacg aagatcgccg gcgagtctcg tctcgacctc tccctcgagt
     1981 gcttcaacgt cctgaacacc ggagagacgc tcaccatcga cgactactac ggcgagtacc
     2041 gcagcggcac ctggcgcaag agcgccacct acggcgccac actcagcatc gagaccccgc
     2101 gccagctccg gctcggcgcg cgcttcagct tctagcccct ccctcctcct ctttcttcgc
     2161 agggggcggc ccgtggccgc cccccccttt tttcgccggc gcggcgggcc gccccgcgcc
     2221 ggggagccag acgggcggcc caggagcccc gatgagatcc tcccgcgcgc tccccctcct
     2281 cttcgccttc tcgctccttc tcctcggcgc gaaggctcct ttcgccctcg gcgccgaggt
     2341 ccccgcgcgg ggccacctcg tcctgatcgg aggcggcgac aagccccccg aggtgatgcg
     2401 gaagttcgtc gagctggccg gggggaagga cgcgccgatc gtcgtaatcc cgacggcgtc
     2461 ctcggaagcc gacgcccccg attactacga gaagctcttc cgcgaagagc tcggctgcct
     2521 cgacgccgtc tcgctccgga tccggagccg cgccgacgcc tcgcgccccg acttcgtcgc
     2581 cctagcccgg aaggcccgcg gagtcttctt cggcggcggc gaccagatcc ggatcacgaa
     2641 cgccctcctc gggacgcccg tcggcgacgc gatcgccgcg gccttcgccg gaggcgccgt
     2701 cgtcggcggc acgtcggcag gcaccgcctg tcagagcgag aagatgatca ctggggaggg
     2761 ggacttcacc caggtgcgga cgcggagcgt cgagctctgg caaggcctcg gcttcctccc
     2821 gcccgacgtc gtcgtcgacc agcacttcct ccgccgccag cggcagaacc ggctcctctc
     2881 cgtcgtcctc gagaacccat cggtcctcgg catcggcgtc gacgaggaga ccgcgatctg
     2941 ggtccgcccc gacgggactc tccaggtcct cggcgagagc ggcgtcatgg tcttcgacgc
     3001 gaagggcgcc gcgctctcgc gccagcctcg ccccaccggc caggacctcc tcggcgtccg
     3061 tggcctctcc ctccacctcc tcctccccgg cgaaaccttc aacctgagct cgcgccgccc
     3121 cctttgaccg gtgccaggcg tgaaatcgtg cagtattcga ggaaaggacg gtaatacatg
     3181 atttcacgcc tggcaccgct tcgggtggca ccgattcagg ttccgtttcg gggggcacgg
     3241 gtcgtggccc tcgcggcgtt cgtccttcta tccggcgtgg tctcggcgga gccgcccggg
     3301 tgggatcagg ggcggtcggt gtacgagcgg cgggtccggc cggcggaagc agccgagaag
     3361 gtcgactggg aggcccgggt gacggcgctc ctgcgggagg agcccggcga cctcgtcgtc
     3421 accgccgtcg gcgacgtcat cttcaacgag cggatcagcc acctggaggc ccccgatcgg
     3481 aagaacctcc tccggatcct ccaggaggcg gacgtggcga tcgggaatct cgagttctcg
     3541 atcaacgacc ggcccgagct gcagaaggtc ttctacaact tccgcgcgcc gcgcgagttc
     3601 gcgtgggagc tcgccgccac agggatcagc ctcgtgagcc tggcgaacaa ccatgccctc
     3661 gacttcggtc gcgaggggct cctcgagtgc cttcgcgcgc tcgacctctc ggggatcgcc
     3721 cacgccggcg ccggccggac gctcgccgag gcgcgcgagc ccggcacgaa gcggcccccg
     3781 gggcagaagg ggcgcgtcgc gctcctctcc tacatgcgct actggaccga ccgggaccgc
     3841 tgcgccgacc cgtccgggcc ctgcctcgcg acgatcgacc cggcgacggt cctcgtcgcc
     3901 cggtccgacg ggacggtcga gtcggtcgaa gggccccagg agcgcgacgt gaaggcgatg
     3961 gaggaggacg tcctcctcgc gaagcgccgg cacggccccg tcttcgtggc ctaccacgtc
     4021 cacgacgtga gccacgcccg ggtcttcggc atccaggaca cgacgccgcc gaacgaggag
     4081 atcccgttcc gccgcgcgat cgacgccggc gccgacgcgg tcctcggcag cggcccgcac
     4141 gtcctgcgcg ggatcgagct gcgaaacgga agaccgatct tctacagcct gagcaacttc
     4201 atctaccagt accggacgcc gaaggcgatc ccgacggacc tcccccatca gagagattcc
     4261 gagatgcccc gccccgcgaa cgtctccgtc tgggaccggc gcgactcgcg cgaggtgatg
     4321 gagagcgtcg tcgcgcggat gacgtaccgc gacggcctcc tccggaggct cgagctgatc
     4381 cccgtgacga tcgacgacga ggggccgctc ttcggggtcc cccggctcgc ctcgacgaag
     4441 cgggcgaagg agatcgtcga gctcctccaa cgcctctccg ccccgtacgg gacgaagatc
     4501 gcctggaagg gctggtacgg cgaggtggag cttccggcga tcgcctcccc ttcgccgcag
     4561 accgcgagct agccgcccgg cgcccggcgc ccggcggccg gggacgggca tcgaggccgg
     4621 cttgcgccct gccatccact cacatatact catatagtta tgtgtcatcc cgttcgttgt
     4681 ccgaagtgcg gaaagcccac ctgggccggc tgcggccgcc acgtcgagga ggccctcgca
     4741 ggcgtgccgg tcgagaagcg ctgctcctgc ccgcctcccg attcgctcct ctcgaggctc
     4801 ttcggctctc ggaagtcgtg acggcggcgg accgcgggga ggaggcctcc ctcagcggtc
     4861 cgcggcgagc caggcggtcg cgagcccgag gcgggtgacg cggcgaacct gatccgggtc
     4921 gatcgaagcg ggcgtgtcgc ccgggcggtg gtagtccggg tggaagttcc cgaagaaccg
     4981 gacgaagggg acgcgcgctc gcgcgaagtc ccggtagtcg ctgcccccgt tgactccctc
     5041 ggtccagtcg aggtggaggg ccaggtccgc cgcgcctccg gcccgcgcga ggagcggccc
     5101 gaggtcgggc gcgaagctcg ccacccccgg ctcgaggaac gtgtcggggc gcgcggcagc
     5161 gaggaacgcc tccgcgccgg ggagcttcgc ggaggcgacg aggtcgcgga tctcctccgc
     5221 cttccagggg tgggcgatca tgtcgaagtt caggtagacg gccgtcttcg cgagcggcca
     5281 gagcgggctc cgcacccagt ggcccgagcc gatcttgtcc tcctcctcgc cggtccagag
     5341 cgcgaagacg agcgtccgtt tcggccggac gggagacgac gcgaaggcgc gcgcgatctc
     5401 gaggagggcc gcgatgccgg aggcgttgtc gtccgccccc ggatggatca cgccgtccac
     5461 gcgcccgagg tggtcagcgt gcgcgccgag gacgacggcc tcgtccgcgc gggccggatc
     5521 gctccccggg aggatcccga cgacgttccg ggaaagcccc gtccgctcga gggccgtcgc
     5581 gcgaagcgtc gcggaggcgg ggagcggccg cgagggctcc tccaccttcg gcggcgcgcc
     5641 gaggagcgcc tcgaggacga cgggtgcgca ccggacgacg agcggctcgc gtgccggggc
     5701 ctccgtcatc gaccggaacg cgaacgtcgc gggcttctcg ttccccagga ggcgcgccgc
     5761 ccagtcgttc ccctcgacgg cgaggacggc caccgcgccg agcgccgtcg ccgtctccag
     5821 cttcgcggcc cagcgctcct cggagtcgcc cgcggcgtac ttctccacga gctccttcga
     5881 actccacgcc tcgcccggcg ggagcccctc gcggaggacg acgatcttcc ctttcacgtc
     5941 gaggcccctg tagtcgtcgc gcgcgggcga cgcctcgcgg aggccgtacc cggcaaagac
     6001 gagcggcgcc gagacggagc gggcgggctg cggcgggacg acgacgtcga cgccgtggac
     6061 gaacgtccgt cgcgccgtcg cctcccccgt ccgtcgctcg atctcgacgg agccccccgg
     6121 ctcgcggatc tcgcgcatcg gcaccggctg gaagaacgtg cgcgccccct tcccgtcggc
     6181 ggcgccatac ggcgccacgc cgacgagagc gagctgcgcc gcgccgtact cgagggccgc
     6241 ctcgaggccg tcgctcccga ggcccctccc cgcgaggggc gtcgaggcga ggaacgagac
     6301 gtgcgcgcga agcgcgtccg cgcggacgcc gtcgagcgcg gcgcggagcg gcgcgggaat
     6361 cgcctcgccc gccttcggga ggacgaaccg gggccgagcg gcgtccgcgg ccgaagtcgc
     6421 aggagcagcg gaagccggag acggctgcgc gacggccggt ggagcggcga gcacgccacc
     6481 gaacaccgcg ccgatcgcga gggcgggaac gaacgccgtc ttcctcattt cagcctctcc
     6541 gttcgcagcg tgtacttctc gagccgttcc gggaccacgt cgacgccgat cccggcgccg
     6601 gccgggacct ccacgaaccc ttccgcgtcg aggacgacgg agggcgtcac gaggtcctcg
     6661 cggaaatacc ggctcgtctc cgacgtgtcg cccggaagcg tgaacccctc gaccgtctgg
     6721 agatggatgt tcacggcgcg cccgatcccc gtctcgtcca tcccgcccga ccagaccggg
     6781 atcccgcgct ctctcgcgaa ggcgaggagc cggagcgact ccaggagccc gccgacgcgc
     6841 ccctccttca ggttcaggac ccggcaggcg ccgagctcga cggcggcgcg gaagtcggcg
     6901 aggtcgtgga tcgactcgtc gaggcagagc ggcgtcgcga gctcccgctg gaggagcgag
     6961 tgctcgaaga tgtcggcgta cgagagcggc tgctcgatca tcgtgaggcc gaaggcgtcg
     7021 agcctcgcga ggtgcgcggc gtccgcgagg cggtagtcgc cgttcgcgtc ggccatgagc
     7081 ggaaggtcgg ggaagcgctc gcggaccgcc gcgacgtact cgacgtcctt ccccttcttc
     7141 accttcacct tcacccggtg gtagccgcgc gcgacggcct cctccacctc gcggacgagc
     7201 gccgcgggcg aatcctgcag gccgatgctg atcccggaag ggacccgctt cctcggcccc
     7261 ccgaggagct cgtggagcgg cacgcccttc gctttcgccg cgaggtcgac gagggcgttc
     7321 tcgagcgcgg ccttcgccat ccggttcccg cggacgtggc cgaagcgctc gtcgagctcg
     7381 aggaacgtcc gcccctcctc gacgagcggg aggaggaact ccgcgacgac gtgccgcgcg
     7441 gaggtcgtcg tctcgggcgc gtagaacggg tccgggtcgg cgacgcactc gccccagccg
     7501 accttcccgc cgccctcgac ccggacgagg aacgtctcct tccccgtcca ggtcgccgtg
     7561 gagatcgaga acggcttcac gaacggaagg ctcacgtgga cgaggtcgac ccgctcgacg
     7621 gcggggacgc gctcggcctc gagcggcagg gccgacgcgc gctccgcggg cccgctcact
     7681 tcggcgatcc cgccgtcgcc gccatggcga cgacgcgccg gacggggatc gtcccgtccc
     7741 acgcgacgag cgccttcagg cgcgggtact gccagaggcc gtacatcgcg gtcggctcga
     7801 ggaggatcgc ggcgcgcagc gcatccggcc cctcgagcgg gacccagagg ctcccggccg
     7861 gaagctcgac cggcctcgcc gcgccgcgct tcacgatcgt ccggccgccg taccggccgt
     7921 agaggtcgtc gaactcctcc tcgacgcgga ggagcgcgca cgtctcggcc gtcaccgtcc
     7981 gcggcgccgc gatccgctcg aacccgaggc cgtgccgcgt caggagcgcc ccgatctccg
     8041 cggccgccgc cggctcgacg gcgtagccga gcggcgtcgg aacgctccgc ttcaccgcga
     8101 cgtcggtcat cacgttcgcc gtcggcacac ggatcgtctt ccccgtcgcg agctcgacca
     8161 cggggaactc cgtcaccgtc ccctccgggt tcacccagag cgcgtcggac gggaggaacg
     8221 ccgggagcgg gagcttccgc gccttctcga cgacggcgcg gtcctcgtcg cggtggccgc
     8281 ccccctccag gaaccgccgg aagaggacga ggtaggcgtc gacccgcctg ccgaggtcgg
     8341 cctgcgggtc cggcgccagg tgtcgcacgg ccgcctcgat gatgaacgac aggccgccgt
     8401 aggcgccgac gccgttcagg ccgccgtcgg tgtcgggggc ggagtggcgc tgctcgtcgt
     8461 cggggggcgc gccgccgacc cagtagcgca ggaagcggtg ccccgccttc tcgaggggcg
     8521 cggccgacgc gtcgacccag cgcttcgccg ccgcgacgcg ctcgcggtcg aagtgggggt
     8581 tgttcagccc gtccatcgtg atgtcgggcc acttctccca ccccttccgc gtccactcct
     8641 ccgagtcgcg cccgaactcg tgcgcgtcga cggcgaggtg cggccgcacc cgccgggcca
     8701 cgcggtggag ggcctccgtc tcgggctgga agagggtgac gtggtcgcgg ttcaggtcgg
     8761 cggcggcggc gttcctccgc gtgcccgcct cggcaccgtc ggggttcacc atcggcatca
     8821 cccagaggtc gacctcgggc gggaggagct tcgggtcgcg ggcgaggtcg cgcaggaggt
     8881 agaggagggc gtccttcccc gacacctcgt ctccgtgctg ctgggcgtag aagaggaccc
     8941 ggaacgtcga ggccgcgctc ccgcgcgcgt tcacgaggaa gatgctccgc ccctgcgcgg
     9001 tctgcccctc gacggagacg acgaccggtc ccttcccgtc gatcgtcttc agaaaggccg
     9061 ccatcgcgtc gtacgaggtc gacttcgtca gcgtccggtg gtcctcgggg agggcgccgg
     9121 acgcgacgcc ggggacggcc gcgcccggaa ccggggcgag gagcgccgca aggaggagcg
     9181 gaaacgcctt cacggctctg ctcctagaac ggtccccagt gcatgagcac cggcgggacg
     9241 aggaggagga acgccaggac catcagcgtc agcatgagcg ggaggaacca gcgcgcccac
     9301 ttctcccacg ggatcttcgc cataccgagg acgcccatcg tcacggccga cgtcggaagg
     9361 atcgggttga tcagctcgca gagctggtag gcgaagacca tcgtctggcg cgtcaggccg
     9421 acgaggtcgg cgagcggcgc catgatcggc atcgtcagcg cggcctgcgc cgtccccgag
     9481 tggatgaaga agttgatgac gcactggacg acgaacatga cctgggcggc gacgacgccg
     9541 gggaggtgcg agatcagccc cgcgccgtgg aagaggatcg tgtcgaggac cttcgcctcg
     9601 cgggcgatga tgaggagcgc ccggccgcag gcgatgatca gcgccacgtt gaccatgtcc
     9661 ttcgcgcccg cgacgaacgc cgtcgcgatc cgcgtcccgc cgaggcccgc gacgacgccg
     9721 aggacgagcc ccatcgccag gaagagggcc ccgatctcct cgatgaacca ctgcttcttc
     9781 aggatccccc agacgaggac gcccatcgtg gtcacgaaga gcgagaggac gagcgcgtgc
     9841 cggacggtcc acggctccgc ctcgccgctc gccggggcgc tccgctcgcg cgcgaggtcg
     9901 aggtcgcgca cggggctcgc ctccgggcgc ttcttcacgc gcgccgcgta gaccatcacc
     9961 cacgcgatca tgacgcccgt cgtcacgaac caggtcgcca cccggtagcc gaggcccgag
    10021 tagagcggca ggccgacgag cccctgcgcg atgccgaccg tgaacgggtt gaagaacgcc
    10081 gccccgaagc ccgccgccgc tccgaggaac gggatcgcga cgccgacgat cgagtcgtag
    10141 ccgagcgaga tcgcgagcgg gatgcagatg acgacgaacg ggatgacctc ctcggacatc
    10201 ccgaagaccg agcccgcgag cgagaagacc gccatcagga ccgggatgac gagcttctcg
    10261 aggaacgggc gcgcggcgag cgccgccgtc agcttccgga tcgcgaactc gatcgtcccg
    10321 cccgcctgga cgatgccgaa ggcgcctccg atggcgaaga ggaacgcgat gatgagcgcc
    10381 ccgtcgagga agccgcggat cggcgcgacg acgacccact gagggccgag ccagaccttc
    10441 tcgacctgct ggtaggtccc cggcaccgtc acggtccgcc ccgccttctc gacggtctgg
    10501 tacgtccccg acgggacgag ccacgccagg accaggacga acacgaccag gctcccgacg
    10561 atcaccaggg tgtggggcat ccggaacgta cgcccgctct tgctcgcggc ctcggccatc
    10621 gctgcctctc ccttcccgat tccgttcctt cgaggacgac gcccggggcg gctcccggct
    10681 tctcgagcgc tccgcgggct ccccggcatc atagagaacg ggcgcccgtc cgggggcctc
    10741 gaccggtcca tttcgtgcac atttccggcg ctccggggct cccgggctcc ctgcgacgaa
    10801 gtcgccggag attcgccgtt ccgccccctc catcgctgga ccggaggggg tccggggcgc
    10861 gatgatggcc gatggccgag accccggacc cggggtccgc cagggagaac ccatgacgaa
    10921 ctccgccttc tcgcccgagc ccgtcgtccg gatcgacgac gacggcatct ggggcgatct
    10981 cgaatccgcc tcccggcccg acgccggcga ggtccgcgac ctcctcgcga agtcccggga
    11041 gctgaagggg ctccgcggcc gcgacctcgc cgtcctcgcg aacgtcgccg atcccgccct
    11101 cctcgacgag ctcttccaca ccgcgcgcga ggtcaaggag gcgatctacg gcaagcgcct
    11161 cgttctcttc gcgccgctct acatctcgaa cctctgcgcg aacgagtgca cctactgcgc
    11221 cttccgcgct cggaacacgg cgctcgtccg ccgggcgctg acgcaggagg agatcgcccg
    11281 cgaggtgaag tggctcgagg agcagggcca caagcggatc ctcctcgtgg cgggcgagtc
    11341 gtacccgcgg gaggggttcg actacgtcct ccgctcgatc gagaccgtct acgcgacctc
    11401 gagcggcccg ggcgagatcc gccgcgtgaa cgtgaacgtc gccccgctca ccgacgagga
    11461 gttccgccgg ctgaaggcgg cgaagatcgg gacctaccag ctcttccagg agacgtacca
    11521 ccgcgagacc tacgccgagg tccacgtggc cgggaagaag cgggactacg actggcgggt
    11581 cacggcgatg gaccgggcca tgagggccgg gatcgacgac gtcggcatcg gcatcctctt
    11641 cggcctctac gactggcgct acgagctcct cgccctcatg cagcacgtgg agcacctcga
    11701 gcggacgttc ggcgtcgggc cgcacacgat ctccgtcccg cggatcgagc cggcgaccgg
    11761 ctcggaggtg gcggaccacc cgccgcacgc cgtctcggac ctcgacttca agaaggtcat
    11821 cgcgatcctc cgcctcgccg tcccgtacac cggcctgatc ctctcgacgc gggagtcggc
    11881 cgagacgcgg cgcgaggtcc tcgccctcgg catctcgcag atctccgccg ggagccggac
    11941 gaaccccggc ggctacgagg ccgacccggg cgacccgtgc gccgagcagt tctccctcgg
    12001 cgaccaccgc tcgctcgacg aggtgatccg ggacgtcgcc tcccacggct tcgtcccctc
    12061 cttctgcacc gcctgctacc gcctcggccg gaccggcgcc gacttcatgg acctcgcgaa
    12121 gccgggcgag atcaaggagc actgcaatcc gaacgccctc gcgacgttcc aggagtacct
    12181 cctcgactac ggctcggagg agacgcgccg cgcgggcgag gcgcgtatcg ccgcctcgct
    12241 cgatgagatg gagccgggcc tgcgcgagcg ttcccgttcg atggtcgagg cggtgaagag
    12301 cggccgccgc gacgtcttct gctgaggagt cggcgcgcgt gagctccgcc ccgcgcggtc
    12361 tccggaccca catcggcctc ttcggccgca ggaacgtcgg caagtcgagc gtcctgaatg
    12421 ccctcacgcg gcaggaggtg gccatcgtgt cggccacccc cggaaccacg acggaccccg
    12481 tcgagaagcc gatggagctc ttgccgctcg gcccggtgct cttcatcgac acggcgggcg
    12541 tggacgacga gggggcgctc ggcgagcagc ggatcgcgaa gacgcgagcc gccctcgagc
    12601 ggaccgacct cgctctcctc gtcgcagagg cgcccgaatg ggggccgttc gaggaggagc
    12661 tcttcgggga gattcgcgcc cgttcgatcc cggtcgtcgt cgtcctcaac aaggccgaca
    12721 cggggccggc cccggcaggc ctcgcggggc gtctcgcggc ggccggagtc ccggccgtcg
    12781 cgacgtcgat cccctccggc accggtctcg acgagctccg gcaggcgctc ctcgccgccg
    12841 ctcccgccga cgccgtcgag gggcgccgga tcgtcggcga cctcgtctcc cccggcgacg
    12901 tcgtcgtgct cgtgacgccg atcgacgcgg aagccccgaa gggacggctc atcctcccgc
    12961 aggttcagac gctccgggac ctcctcgaca acggcgcgat ctccgtcgtc gtccgcgagc
    13021 acgagctgca cggcgcgctc gacgcgctcg gccgtccgcc gcgcctcgtc gtcaccgact
    13081 cccaggcgtt ccggcaggtc gcggcggagg tcccccccgg cgttcctctc acgtcgttct
    13141 ccatcctctt cgcccgcttc cagggcgacc tcgcggaaat ggcccgcggc gtgcgcggcg
    13201 tcgaccggct gaaggcgggc gaccgggtcc tcgtcgcgga ggcctgcacc caccacccgg
    13261 tcggcgaaga catcgggcgc gtgaagatcc ccgcctggct ccgcgcccgc accggcgtcc
    13321 cgctcgagtt cgagacggtc caggggcggg acttccccga cgacctctcc cgctacgcgc
    13381 tcgtcgtcca ctgcgggagc tgcatgggga accgccgcca gatgctgtcg cgcctcctgc
    13441 gcggccgccg cgagggggtg cccgtgacga actacggcct gacgatcgcc tactccctcg
    13501 gcatcctcga aagagcgctg gagcctttcc ccgccgccct cgaggcgttc cgcctcgccg
    13561 ccccgccccc gtcctctccg gaggtgacga cgtgatcgac cgcgacggaa tcctcgcctg
    13621 gctccgagag accgacccga cccgcctctc ccgcctctac cggcgcgccg acgcggtccg
    13681 ccgggagcac gtcgggaacg aggtccatct gcgcggcctc gtcgaggtct cgaaccactg
    13741 cgtccgccgc tgcgcctact gcggcatcgc cgccacgaac gacgggatcg agcgctaccg
    13801 gatgacggag gaggaggtcc tcgcctgcgc ccggcaggcc gtcgagctcg gctacggcac
    13861 cctcgtcctc caggccggcg aggactacgg cctgacgcgc gagggcgtct cctcgatggt
    13921 ccgtgcgatc aagcgcgaga ccgggctcgc cgtgacgctc agcctcggcg agcgaccgga
    13981 agaggacctc gtcgcctggc gcgaggcggg ggccgaccga taccttctcc gcttcgagac
    14041 ctccgacgcg gctctctacc aggcgatcca cccggcgatg ccgggccggc ccgaccgatt
    14101 cgcgatcctc gggcgcctcg ccgtcctcgg ctacgaggtg gggagcggcg tcctcgtcgg
    14161 aatcccgggc cagacgtggg agagcctcgc gaacgacctc ctcgtcttcc gccagctcga
    14221 cctcgagatg atcggcgtcg gcccgtacat cccgcacccg gccacaccgc tcggctcggg
    14281 ccgcttcccc gtcgcccccg agggagagca ggtcctcgcc gacgaggaga cgacgacgaa
    14341 ggtcgtcgcc ctgacgcgcc tcgttcgccc cgacgcgaac atcccgtcga cgaccgcgct
    14401 cgcgacgctc gacccctcgt gggggcgcga gcgcggcctc cttcgcggcg cgaacgtcgt
    14461 catgccgaac ctcacgccgc cggcttaccg gcaactctac gagatctacc cgggcaaggc
    14521 ctgcgtcaac gagacggccg aggcgtgcgg cagctgcctg ccgaatcgaa tccggatgat
    14581 cgggagggtg ccggggaagg gacccggagg gcgaagacgg accgtcgcga gcggagcgct
    14641 ctcctccgtc ctcggctgac ctccccggcc tcccccgcgg accacaggct cgccctcgcc
    14701 gctcgcggta cgggctcgct cctcctcccc gcgctcttcg cccctcgagg tcggctacca
    14761 tcccgtgacc ggaaggagcc gacatgggga agtggacggg gaggtcggtc gttcgcgtcg
    14821 ccgcgctcgc cggcgcgctg atggtgccgc attcgctgag cgcggacctc gaggacgacc
    14881 tcgagcgggc gtgggtcggc gcgtgggtcg tcacgaaggt cgagaccgcg tccgactgct
    14941 cgggcaccta cacgaacgtc gaggtgcggg gcctccggag cacgagccgc gggagccggc
    15001 gattcggcga aggggagctc gcgcggatcg acaagctgaa ccagaagtcg gaccgggtcg
    15061 acctctacct ctccgtcgcc gagccgatcc tcgtgccgcg cacggacggg c
//
LOCUS       DAOTEZ010000053        15022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2728_length_15022_cov_5.518875, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000053 DAOTEZ010000000
VERSION     DAOTEZ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15022
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2728_length_15022_cov_5.518875"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="P5164_04355"
     CDS             complement(<1..217)
                     /locus_tag="P5164_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HRY43160.1"
                     /translation="MSTRVVLHEVGPRDGLQVEKVVVPTETKLRWIAALCEAGLDVVQ
                     VGSFVHPEKVPQMADTDALFRTLTAPGA"
     gene            complement(226..1344)
                     /locus_tag="P5164_04360"
     CDS             complement(226..1344)
                     /locus_tag="P5164_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HRY43161.1"
                     /translation="MSAYKIAWLPGDGVGVEVMEAARITLDALKLDAAYTHGDIGWEF
                     WRTEGESFPQRTIDLLKASDAAMFGAITSKPVKAAEQELVPELQGKGLTYRSPIVRMR
                     QMFDLYVCLRPCKAYPGNPLNFKDTIDLTIFRENTEDLYAGVEFSPVPDALATTLKAL
                     SKPFAHFADLAGDQYAVSCKVNTRKGSERIVRAAFEYARKFGRKKVTIVHKANVVRAT
                     DGLFFEEAKKVAADFPEIAVDDANIDAICMWLLKNPMSYDVLVAPNLYGDIISDLAAQ
                     MVGGLGFGCSGNIGKKLAVFEPSHGSAPKYAGQYKVNPLATILAAKMMLDFLGEPEKG
                     AKLEAAVAKVIAEGKVRTYDMGGSATTLEMGEAVARAL"
     gene            complement(1440..3836)
                     /locus_tag="P5164_04365"
     CDS             complement(1440..3836)
                     /locus_tag="P5164_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY43162.1"
                     /translation="MSPEGRGEEAPGSDLTLFEQVARAIDDYILVVDLEGRLTHVNDA
                     AVERSGWAREELLGRPWTVFLPPDGREALAARIAEETRAGGFRGDVRNATRAGEVFWV
                     ALRTSVLRHDGRAVGFVSVSRDIGEQKRVEAELTAARDAAETASRAKSEFLANISHEI
                     RTPMNAVIGMATLLLDTALTPEQRDYVETIRTSGDTLLALLNDVLDFSKMESDRLELE
                     ARPFSFREAVEDSFDLVAPSAAAKGLDLAYRVDPSVPASLLGDVTRVRQILLNLLSNA
                     VKFTASGEVLLRASARPAAEGSHRVEIVVRDSGIGIPKERLDRLFRSFSQVDPSTTRQ
                     YGGTGLGLAICKRLAEKMGGGIEAESSGRGSTFRVSLVVPAGPPLREASVPGLDRLAG
                     QKVLVVDDGPASRDTLFETLADWGMHPRATSSPREALAWVDSGEPFDLGLIDEEMAEV
                     DGRRFAESLRRFRDSDALPLVLVTSRGRRAGGARAPFSAVVARPVRPRALASVLAGLR
                     DDSVSRRRRRSSPALPKELGTSHPLRVLLAEDNAVNQKVALSYLARMGYRADVARTGI
                     EALEAVRRNVYDVVLMDVQMPEMDGLEAARRIRSFAPAEDGPWIIALTASAMTGDAER
                     CLAAGMNDYLAKPVRGEDLAAALRRAPRSASSTGPASALTAGRPVLLPGPLAKLRELE
                     RPGETGFLASLLEIFRRDTPIRLEEMERAAAAADAEALRRAAHTLKSSAATLGGARVE
                     ALATRTEVLAMTPATLAEAAAFLPALRSESAALLAALGLAEGEEGARLSLDPGDYGRS
                     "
     gene            complement(3833..4417)
                     /locus_tag="P5164_04370"
     CDS             complement(3833..4417)
                     /locus_tag="P5164_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43163.1"
                     /translation="MKADASDRIDPRAMVKGTMLRAHLSWATSRFGTGYEPVRQRLSE
                     PVLALLSRPILPTDWIPVSSLIELDRAIADAAGEPPETTWLALGRHSAALNLTGVYKS
                     FISSEPHRFFERMTVLHHQFQSFGKSVYERTGDRSGRIRIESPSTYSRVYCVSGKGYY
                     EEALRLLQAPGPIEVRETECATAGAAACVFELAW"
     gene            complement(4464..5714)
                     /gene="ispG"
                     /locus_tag="P5164_04375"
                     /gene_synonym="gcpE"
     CDS             complement(4464..5714)
                     /gene="ispG"
                     /locus_tag="P5164_04375"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRY43164.1"
                     /translation="MTTNAKRHATVAVKVGKVTVGGGAPVVVQSMTSTDTADVEATTK
                     QCRELAEAGSELVRVTVNLPEAAAAVPEIRKRLDDAGCDVPLIGDFHYNGHTLLTSFP
                     EMAKALAKYRINPGNVGTGRRRDEQFATICAVARDNAKAVRIGVNGGSLNQELVLAKM
                     QENTDRDLGRTPEEILNECMVLSALQSTELALENGLAREQIVISCKVSRPPELISLYR
                     DLASKTDQPLHLGLTEAGMGTKGLVWSSAAMGVLLAEGIGETIRVSLTPRPGGDRREE
                     VYAACELLQALGLRSFAPSVTACPGCGRTTSTTFQELAERIQGYVREKMPAWKAEREG
                     VETMTLAVMGCIVNGPGESKAASIGISLPGTGEAPSCPVFVDGKKFTTLKGSYEELAA
                     AFQALVDDYVATKYPLRAGAGRRG"
     gene            complement(5827..7929)
                     /locus_tag="P5164_04380"
     CDS             complement(5827..7929)
                     /locus_tag="P5164_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43165.1"
                     /translation="MPYRPARAARRLASALVLALLLPAPRGLAEDVGATAGPRVSIPL
                     ADYESLRKLREAPSLTVVDTLRVSGSFASRTLALSLSGRSSGTLPAATLLESIEGAAL
                     AGCDGSALVSRGTDAAYTLTPLGPRFEVRCRLVPSSGDRLSFVAGRAVLFPEGEVADG
                     EFLPGPDPGDGTRPFTVVRRIAAAGEALAPTATGRYRITLLPDETRFRFTIEVRNPNR
                     GRAPFDLLLGSGERVQEVVAPGPYDVKEGRTRFDLPPGDGTIVLTGTLGGTEFRAPVD
                     AALSYLLLESHPLLRAEARTAAKRISPQETGMPREFRGGQAFLLGKGDAFAWSVKRLE
                     ALRSTSYAVRATAQTLFVPADGPVLGQTLFEIDNQGASDLALPPSPEPTYAAFGDEAV
                     FLTRNEKGELWLPLASGPQALLLQSRQPLGRALGFATGRLAVPHLPVPSTRYAVELRY
                     PDAWVPLYERFGGESRFAAPSIEASVVFLLLSGWLFALLGALRLGPRGAALLAGILAL
                     SAFASATAAYVVVAAAAVVTVFWVAAVARERRPRLRTLVALGALGAFAALVVLAVAIP
                     SGLLRSRGPQAPPAEVAFYSESASAPVARKAVRSDAPAAPAPEPQASGGATYEGLPAK
                     VELPRGARSTWWSREMLAADAAPVVRVVLAGRTLVSLAQGLVVLAALGLLWRSRAGLR
                     DGLRALRARGRTEGPAGR"
     gene            complement(8039..8812)
                     /locus_tag="P5164_04385"
     CDS             complement(8039..8812)
                     /locus_tag="P5164_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017744414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRY43166.1"
                     /translation="MTTDAWDPSRYAKFGAERAAPFFDLLDLVEPRPAMRVLDLGCGT
                     GELTRALHERLRARETLGLDRSGRMLEKSAAFVAPGLRFEEGTIEEYAGDAPLDLVFS
                     NAALHFVADHESLWPRLAALLAPGGQLAVQLPANQDHPTHAAAREVAAGPPFAAALGG
                     WAHPTHVLPPEAYAVLLHRLGFAKQTVRLAVYTHLLESPESAVEWVRGSILTEYESRL
                     GPELFPDFLASYRARLLPRLGEERPYFFTFKRILVHGAL"
     gene            8852..9958
                     /locus_tag="P5164_04390"
     CDS             8852..9958
                     /locus_tag="P5164_04390"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HRY43167.1"
                     /translation="MSAAKKRFLLYGANGYTGRLILEAALEKGLLVTVAGRRREAIEP
                     LAKERGLPFLVFGLDEAPRRLAERVAPFGALLLAAGPFSRTSAPALAACLAARVPYLD
                     ITGEIAVLEAVFARHPEAVRAGVPLLPGVGFDVVPSDCLAAALAEALPGAVRLELAFR
                     GFGISGGTARTMLEGLPKGGAARVDGKLVPVPPAWKTRTVPFSDRPRLAVTIPWGDLS
                     TAYRSTGIPNIETYIAMPPARVAALRWAGPLLPLAGLLPLQRVVGPWVRNAVSGPSAE
                     ERARERSLLWGRVEDGEGRSVEGTVETLEGYALTAETATLAVERVLRRSVEPGAFTPS
                     RAFGARCIEKVRDTKMSVPARAADAAPRRASRKE"
     gene            complement(9968..10444)
                     /locus_tag="P5164_04395"
     CDS             complement(9968..10444)
                     /locus_tag="P5164_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43168.1"
                     /translation="MKGTPSSGLWVLLLLVAVGAVVFRLARRGTVSDDAIVRDADRTV
                     VAEYVRLVGEGKYAEAWERCLTKSYRDEVPREKFVAAHEKRRAETGALAGAKLLRPRV
                     SRNLFTRTRTLHLLYELSYSGKSVPEYAVLNDADGAFRIEGTYHEAAGETLDFLLW"
     gene            10614..11033
                     /locus_tag="P5164_04400"
     CDS             10614..11033
                     /locus_tag="P5164_04400"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRY43169.1"
                     /translation="MARPDPHPPEVFERLRRRGPETRIAVVGASNAPEKYGNVIVRNL
                     AAKGYTVLPVNPRETEIAGLPAWPNLESVPGPVHIVNVVVPPQVTLGVLAKSAELGLP
                     AVWLQDGSFDDAVLEAAAKAPFETVYDACIMVASNFG"
     gene            11056..12513
                     /gene="purF"
                     /locus_tag="P5164_04405"
     CDS             11056..12513
                     /gene="purF"
                     /locus_tag="P5164_04405"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRY43170.1"
                     /translation="MSGETESIETDGFREECGVFGIYGHPDAANLAYLGLYALQHRGQ
                     EAGGIAAWNGRRIAVEKGMGYVADLFDEARLARLPGRSAVGHVRYSTAGASLLENAQP
                     IVYNTNKGPLAIAHNGNLVNAEELRAELEQEGSIFTTSSDTEVFLHLMARSRAESLVD
                     ALRDALGRARGAYSIVLLAGEKVLAARDPQGFRPLTIGRLGDAWVVASETCAFDLIDA
                     EPVRDVEPGEIVILDAGGVASASFAMGRRTAFCVFEHVYFARPDSNVFSRSVAESRRA
                     FGRRLAREHAVAADVVVPVPDSGIFAALGYAEESGIPYGMGLVRNHYVGRTFIEPKQS
                     IRSFGVKVKLNPVKSVVGGKRVVLVDDSIVRGTTSKKLVRLLKSAGATEVHMRISSPP
                     TTHPCHYGIDTPRRKELIAATHAMEEIRRFVEADSLGYLSLEGMLGAFGKGEGEACAA
                     CFSGRYPVPFAAALEQQPLFGEDEVLPSSGSPEKR"
     gene            12572..13612
                     /gene="purM"
                     /locus_tag="P5164_04410"
     CDS             12572..13612
                     /gene="purM"
                     /locus_tag="P5164_04410"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRY43171.1"
                     /translation="MPNDTPLTYAASGVDIDAKMSAVARAKEAIRSTFTSGVVGDVGG
                     FGGLFRPDFRGMQDPLLVASADGVGTKIRVAIAADVHGTVGQDIVNHCVNDILVQGAR
                     PLFFLDYVATGRMRREVIAEVIGGVAVACRENGCALLGGETAEMPGMYADGDYDLAGT
                     IVGVVDRPKLLDGSRVAEGDLLLGLPSSGLHTNGYSLARMLFFERLGLGPHDAVPELG
                     TTIAEALLAVHRSYLAALLPLVEDGLLSAMSHVTGGGFRDNLPRVLPEGLRAVVTPGA
                     WAWPALFRFIREKGSVPEEEMLRVFNCGVGMVLFVAPERAAEVRRRLAASGETVLDVG
                     RVEKGPRSVRFA"
     gene            13609..14280
                     /gene="purN"
                     /locus_tag="P5164_04415"
     CDS             13609..14280
                     /gene="purN"
                     /locus_tag="P5164_04415"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRY43172.1"
                     /translation="MTAVGVSFEAPVPWFPLPARLAVLLSGRGSNFVALADACESGAL
                     PARIVLVLSDRADAAGLARARARGLEAALEERGGETRAAHEARLAARIETAGADVVCL
                     AGFMRVLSPAFVARFERRVVNVHPSLLPAFPGLEAQHQAFEHGVKVAGATVHLVDAGT
                     DTGPIAGQEAVAVGPGDDAERLAARILEVEHRLYPATLAALLAGGWSIEGRRLRFSGP
                     GSVEA"
     gene            14959..>15022
                     /locus_tag="P5164_04420"
     rRNA            14959..>15022
                     /locus_tag="P5164_04420"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         2034 a         5383 c         5504 g         2101 t
ORIGIN      
        1 ccgcccccgg cgcggtcagc gtccggaaga gcgcgtcggt gtcggccatc tgcgggacct
       61 tctccgggtg gacgaaggag ccgacctgga cgacgtcgag gcccgcctcg cagagcgcgg
      121 cgatccagcg aagcttcgtc tccgtcggga cgacgacctt ctcgacctgg aggccgtccc
      181 tcgggccgac ctcgtggagg acgacgcgcg tcgacaccgg agtccctaga gagcccgggc
      241 cacggcctcg cccatctcga gcgtcgtcgc cgagccgccc atgtcgtacg tgcgcacctt
      301 gccctccgcg atgaccttcg cgacggccgc ctcgagcttc gcccccttct ccggctcgcc
      361 gaggaagtcg agcatcatct tggcggcgag gatcgtcgcg agagggttca ccttgtactg
      421 cccggcgtac ttcggcgccg agccgtggct cggctcgaag accgcgagct tcttcccgat
      481 gttccccgag cagccgaagc cgaggccgcc gaccatctgg gccgcgaggt ccgagatgat
      541 gtcgccgtag aggttcgggg cgacgaggac gtcgtagctc atcgggttct tcaggagcca
      601 catgcagatc gcgtcgatgt tcgcgtcgtc gacggcgatc tcggggaagt cggcggcgac
      661 cttcttcgct tcctcgaaga agaggccgtc cgtggcgcgg acgacgttcg ccttgtggac
      721 gatcgtgacc ttcttccgtc cgaacttccg cgcgtactcg aaggcggccc tcacgatccg
      781 ctcggagccc ttgcgggtgt tcaccttgca ggagacggcg tactggtcgc ctgccaggtc
      841 ggcgaagtgg gcgaacggct tgctgagcgc cttgagcgtc gtggcgagag cgtcggggac
      901 gggcgagaac tcgacgccgg cgtagaggtc ctcggtgttc tcgcggaaga tcgtcaggtc
      961 gatcgtgtcc ttgaagttga gcgggttccc cgggtaggcc ttgcaggggc gcaggcagac
     1021 gtagaggtcg aacatctgcc gcatcctcac gatcggggag cggtaggtga ggcccttgcc
     1081 ctgcagctcg gggacgagct cctgctcggc cgccttcacg ggcttgctcg tgatggcccc
     1141 gaacatcgcg gcgtcggagg ccttcaggag gtcgatcgtc cgctgcggga acgactctcc
     1201 ctccgtgcgc cagaactccc agccgatgtc gccgtgcgtg taggcggcgt ccagcttcag
     1261 ggcgtcgagg gtgatgcggg cggcctccat gacctcgacg ccgacaccgt cgccgggaag
     1321 ccatgcgatc ttgtacgcgc tcatcggatt acgctcccgc gcgcgcgccc tcgagggggt
     1381 gctcgcgcat cggagagtct atcgccgcgg aggccgcttc cgggcctccc gagaagggct
     1441 caggaacgcc cgtagtcgcc cggatcgaga gagagccggg cgccctcctc gccttcggcc
     1501 aggccgaggg cggccagcag cgccgcggac tcgctccgga gggccgggag gaaggccgcc
     1561 gcctcggcga gcgtcgccgg ggtcatcgcg agcacctccg tgcgggtcgc gagagcctcg
     1621 acccgggcgc ctccgagcgt cgccgcgctc gacttgaggg tgtgggccgc cctgcgaagg
     1681 gcctcggcgt ccgccgcagc ggcggcccgc tccatctcct ccagccgaat cggcgtgtcg
     1741 cggcggaaga tctcgaggag ggaggcgagg aagcccgtct ccccaggccg ctccagctcc
     1801 cgcagcttcg ccaggggacc cggaaggaga accggccggc cggcggtgag ggccgaggcc
     1861 ggcccggtcg acgaggccga gcgcggagca cggcggaggg ccgccgcgag gtcctctccc
     1921 cggaccggct tggcgaggta gtcgttcatc ccggccgcca ggcagcgctc cgcgtcgccc
     1981 gtcatggcgc tcgccgtgag ggcgatgatc caggggccgt cctcggcggg ggcgaagctg
     2041 cggatgcggc gcgcggcctc gaggccgtcc atctccggca tctggacgtc catcaggacg
     2101 acgtcgtaga cgttccgccg gaccgcctcc agggcctcga tgccggtgcg ggcgacgtcg
     2161 gcgcgatagc ccatccgggc gaggtaggag agcgccacct tctggttgac ggcgttgtcc
     2221 tcggcgagga ggacgcggag cgggtggctc gttccgagct ccttcggaag ggcgggcgag
     2281 gagcgcctgc ggcgccgcga gacgctgtcg tcgcggaggc ccgcgaggac ggaggcgagg
     2341 gcgcggggcc ggacgggccg cgcgacgacg gccgaaaacg gcgcccgcgc gccgccggcc
     2401 ctccggccgc gggaggtgac gaggacgagc ggcaaggcgt cggagtcccg gaaccggcgg
     2461 agcgattcgg cgaacctccg gccgtccacc tcggccatct cctcgtcgat gaggccgagg
     2521 tcgaacggct ccccggagtc gacccaggcg agcgcctcgc gcggcgagga ggtggcgcgc
     2581 gggtgcatcc cccagtccgc cagcgtctcg aagagcgtgt ctcgggaggc cgggccgtcg
     2641 tccacgacca ggaccttctg cccggccagc cggtcgagcc cggggacgga cgcctcgcga
     2701 agaggggggc cggccgggac gacgagcgag acgcggaacg tcgagccccg gccggagctc
     2761 tccgcctcga tgcccccgcc catcttctcg gcgagccgct tgcaaatggc gaggccgagc
     2821 ccggtccctc cgtactgcct cgtcgtcgag gggtcgacct ggctgaagct ccggaagagc
     2881 cggtcgaggc gctccttcgg gatcccgatc cccgagtcgc ggacgacgat ctccacgcgg
     2941 tgggagccct cggccgccgg ccgggccgaa gcgcggagga ggacctcgcc cgaggcggtg
     3001 aacttcacgg cgttcgagag gaggttcagg aggatctgcc ggaccctcgt cacgtcgccg
     3061 aggaggctcg ccgggacgga cgggtcgacc cggtaggcga ggtcgaggcc cttggccgcg
     3121 gcggaggggg cgacgaggtc gaaggaatcc tccacggcct cccggaacga gaacgggcgg
     3181 gcttccagct cgagccggtc ggactccatc ttggagaagt cgaggacgtc gttcaggagc
     3241 gcgaggagcg tgtcgccgct cgtccggatc gtctcgacgt agtcgcgctg ctcgggggtg
     3301 agcgccgtgt cgagcagcag cgtcgccatc ccgatgaccg cgttcatcgg cgtccggatc
     3361 tcgtgactga tgttcgcgag gaactcggac ttcgcgcggc tcgccgtctc ggcggcgtcg
     3421 cgcgccgcgg tcagctcggc ctcgacgcgc ttctgctccc cgatgtctcg ggagaccgag
     3481 acgaacccga cggcccgccc gtcgtggcgg aggaccgacg tccggagcgc gacccagaac
     3541 acctcgcccg ccctcgtggc gttccggacg tcgccgcgga agcccccggc ccgcgtctcc
     3601 tcggcgatcc gggcggccag cgcttcgcgg ccgtccgggg gaaggaagac cgtccacggt
     3661 cgtccgagaa gctcctcccg cgcccagccg gatcgctcga cggccgcgtc gttcacatgc
     3721 gtcagccgtc cctcgaggtc gacgacgagg atgtagtcgt cgatcgcccg cgcgacctgc
     3781 tcgaaaagcg tgaggtcgct tcccggggcc tcttcccccc ggccttccgg gctcaccagg
     3841 cgagctcgaa gacgcaggcc gcggcgcccg ccgtggcgca ttcggtctcg cggacctcga
     3901 tcggcccggg agcctggagg agccggagcg cctcctcgta gtagcccttc cccgagacgc
     3961 agtagacgcg cgaatacgtc gaggggctct cgatccggat ccggcccgag cggtctccgg
     4021 tcctctcgta gaccgacttc ccgaacgact ggaactggtg gtgcaggacc gtcatcctct
     4081 cgaagaagcg atgcggctcg ctgctgatga agctcttgta gacgcccgtc aggttcagcg
     4141 cggcggagtg ccgcccgagg gcgagccacg tcgtctcggg gggctctccg gcggcgtccg
     4201 cgatcgcgcg gtcgagctcg atgagcgacg agaccgggat ccagtcggtc gggaggatgg
     4261 ggcgcgagag gagggcgagg accggctcgg agaggcgctg gcggacgggc tcgtaccccg
     4321 tcccgaagcg gctcgtggcc cagctcaggt gggccctcag catcgttccc ttcaccatgg
     4381 cccgagggtc gatccggtcg gacgcgtcgg ctttcatcga cggtgaaggt tatcgcgatc
     4441 gggccgggcc gtggctgccc ccgtcagccc ctccgcccgg cgccggcgcg gagggggtac
     4501 ttcgtcgcca cgtagtcgtc gacgagcgcc tggaacgcgg cggcgagctc ctcgtacgac
     4561 cccttcagcg tcgtgaactt cttgccgtcg acgaagacgg ggcagctcgg cgcctcgccc
     4621 gtccccggca ggctgattcc gatgctcgcg gccttcgact cgccggggcc gttcacgatg
     4681 caccccatga cggcgagcgt catcgtctcg accccttcgc gctcggcctt ccaggccggc
     4741 atcttctccc gcacgtagcc ctggatccgc tcggccagct cctggaacgt cgtcgaggtc
     4801 gtccggccgc agccggggca ggcggtgacg ctcggcgcga acgagcgcag cccgagcgcc
     4861 tggaggagct cgcaggccgc gtacacctcc tcgcgccggt cgcctcccgg gcgcggcgtg
     4921 agcgagacgc ggatcgtctc gccgatcccc tcggcgagga ggacccccat cgcggcggac
     4981 gaccagacga gccccttcgt ccccatcccg gcctccgtga ggccgaggtg gagcggctgg
     5041 tcggtcttcg aggcgaggtc gcggtagagc gagatcagct ccggcgggcg ggagaccttg
     5101 caggagatga cgatctgctc cctcgcgagg ccgttctcga gcgcgagctc cgtcgactgg
     5161 agagcggaga ggaccatgca ctcgttcagg atctcttccg gcgtcctccc caggtcgcgg
     5221 tcggtgttct cctgcatctt cgcgaggacg agctcctggt tcagggagcc gccgttcacg
     5281 ccgatgcgca cggccttcgc gttgtctcgg gccacggcgc agatcgtggc gaactgctcg
     5341 tcgcgcctcc gccccgtccc gacgttgccg gggttgatcc ggtacttcgc gagggccttc
     5401 gccatctccg ggaacgacgt caggagcgtg tggccgttgt agtggaagtc gccgatcagc
     5461 gggacgtcgc agccggcgtc gtcgaggcgt ttccggatct cggggaccgc cgcggccgcc
     5521 tcggggaggt tcaccgtgac gcggacgagc tccgagcccg cctcggcgag ctcccggcac
     5581 tgcttcgtcg tcgcctcgac gtcggccgtg tcggtcgacg tcatcgactg gacgacgacg
     5641 ggggcgcccc cgcccaccgt caccttcccg accttcaccg ccaccgtggc gtggcgcttc
     5701 gcgttcgtcg tcaatgaggg cctcccgcgg gacgcgtccc gcgcgcgcgt cacgttagca
     5761 aagccgatcg gtccctctcc gccgccggcc cgccgggcgg atgagcgcga tcgccgccat
     5821 ggggcgtcag cggcccgccg gaccctccgt tcggccccgg gcccggagcg cccggagccc
     5881 gtcgcgcagc cccgcgcgcg agcgccagag gaggccgagc gcggcgagga cgacgagccc
     5941 ctgcgcgagg gagacgagcg tccggccggc gaggacgacg cggacgaccg gcgcggcgtc
     6001 ggccgcgagc atctcgcggc tccaccacgt gctccgggcg ccccggggca gctcgacctt
     6061 cgcggggagc ccttcgtagg tcgcgccgcc cgaggcctgc ggctccggtg cgggggccgc
     6121 gggcgcgtcg gagcgcaccg ccttgcgggc gaccggcgcc gacgcggact cggagtagaa
     6181 ggcgacctcg gcgggagggg cctgcgggcc ccgcgagcgg aggaggccgg aggggatcgc
     6241 gactgcgagg acgacgagcg cggcgaacgc accgagcgcg ccgagcgcga cgagggtgcg
     6301 gaggcgcggc ctcctctctc tcgcgacggc agcgacccag aagaccgtga cgacggccgc
     6361 cgcggcgacg acgacgtacg cggcggtggc cgaggcgaac gccgagaggg cgaggatccc
     6421 ggccaggagg gccgcgcccc gcggcccgag gcggagcgcg ccgaggaggg cgaagagcca
     6481 tcccgagagg agcaggaaga cgacgctcgc ttcgatcgac ggcgccgcga agcggctctc
     6541 gccgccgaag cgctcgtaga gcgggaccca ggcgtcgggg tagcggagct cgacggcgta
     6601 gcgcgtcgag gggaccggca ggtgcggcac ggcgagcctc ccggtcgcga agccgagcgc
     6661 ccggccgagg ggctggcggc tctgaaggag gagcgcctgc ggcccggagg cgagcgggag
     6721 ccacagctcc cccttctcgt tcctcgtcag gaagaccgcc tcgtcgccga aagccgcgta
     6781 ggtcggctcc ggagagggcg gcagcgcgag gtcggaggcc ccctggttgt cgatctcgaa
     6841 gagggtctgc ccgaggacgg ggccgtcggc ggggacgaag agcgtctgcg ccgtcgcgcg
     6901 aacggcgtag ctcgtcgagc ggagcgcctc gaggcgcttc acgctccagg cgaacgcgtc
     6961 gcccttcccg aggaggaacg cctgcccgcc ccggaactcg cgcggcatcc ccgtctcctg
     7021 cgggctgatc cgcttcgccg cggtccgcgc ctcggcccgg agcagcgggt ggctctcgag
     7081 gaggaggtag gagagcgcgg cgtcgaccgg tgcgcggaac tccgtcccgc cgagggtccc
     7141 cgtcaggacg atcgtcccgt cgccgggcgg gaggtcgaag cgcgtcctcc cttccttcac
     7201 gtcgtaaggg ccgggcgcga cgacctcctg gacccgctcg ccgctgccga ggaggaggtc
     7261 gaacggagcg cggccccggt tcgggttcct cacctcgatc gtgaagcgga agcgggtctc
     7321 gtcggggagg agcgtgattc gatagcgccc cgtcgccgtc ggcgcgaggg cttcgccggc
     7381 cgcggcgatc cgccgcacga cggtgaaggg cctcgtcccg tcgcccgggt cggggccggg
     7441 gaggaactcc ccgtccgcga cctcaccttc cgggaagagg acggcacggc cggcgacgaa
     7501 ggagaggcgg tctcccgaag aaggcacgag ccggcagcgg acctcgaagc gcgggccgag
     7561 cggcgtcagc gtgtaggccg cgtcggtgcc gcgcgagacg agggcgctcc cgtcgcaccc
     7621 cgcgagcgcg gctccctcga tcgactcgag gagtgtggcg gcaggcaggg tcccggagga
     7681 gcgacccgag agggagagcg cgagcgtccg cgacgcgaag ctcccggaga ccctcagcgt
     7741 gtcgacgacc gtgaggctcg gcgcctcgcg cagcttgcgg agggactcgt agtccgcgag
     7801 agggatcgag acccgcggtc cggccgtggc gccgacgtcc tcggcgaggc ctcgcggggc
     7861 cgggaggagg agggccagga cgagcgcgga agcgagcctt cgggcggcgc gcgcggggcg
     7921 gtacggcatc gtcatcacct cgggtgcttc gactctactc gcccgcggcg gttcccggca
     7981 cgggtcggac gcgtgcccgg cgtcacgggg cggctcccgg cggcccgggc cgcgccggtc
     8041 agagggcgcc gtggaccagg atgcgcttga acgtgaagaa gtacggccgc tcctcgccga
     8101 gccgggggag gaggcgggcg cggtacgacg cgaggaaatc cgggaagagc tctgggccca
     8161 ggcgcgactc gtactcggtg aggatcgagc cccggaccca ctcgacggcg ctctcgggag
     8221 actcgaggag gtgcgtgtag acggcgagcc ggaccgtctg cttcgcgaag ccgagccggt
     8281 ggagcagaac cgcgtaggcc tcgggcggga ggacgtgcgt cgggtgagcc cagccgccga
     8341 gcgccgcggc gaacggcggc ccggcggcca cctcccgggc cgccgcgtgc gtcggatggt
     8401 cctggttcgc cgggagctgg accgcgagct gtccccccgg ggcgaggagg gcggcgaggc
     8461 ggggccagag cgactcgtgg tccgcgacga agtggagcgc ggcgttcgag aagacgaggt
     8521 cgagcggggc gtcgcccgcg tactcctcga tcgtcccctc ctcgaaccgt aggcccggtg
     8581 cgacgaacgc ggcgctcttc tcgagcatcc ggccggaccg gtccaggccg agcgtctccc
     8641 gcgcgcggag ccgttcgtgg agggcccgcg tcagctctcc cgtcccgcag ccgaggtcga
     8701 ggacgcgcat cgcgggccgc ggctcgacga ggtcgaggag gtcgaagaac ggcgcggcgc
     8761 gctcggcccc gaacttcgcg taacgagatg ggtcccaggc gtcggtcgtc acgggcacga
     8821 tcgtagcgga cggcgtacga tgggaccggt cgtgtctgcc gccaagaagc gcttcctcct
     8881 ctacggggcc aacgggtaca cgggacggct gatcctcgaa gccgcgctcg agaagggtct
     8941 cctcgtcacc gtggcgggcc gccggcgcga ggcgatcgag ccgctcgcga aggagcgtgg
     9001 cctcccgttc ctcgtcttcg gtctcgacga ggcgccgcgg cgcctcgcgg agcgcgtcgc
     9061 gccgttcggg gcgctcctcc tcgcggccgg acccttctcc cggacgagcg caccggcgct
     9121 cgcggcgtgc ctcgcggccc gcgtcccgta cctcgacatc acgggagaga tcgccgtcct
     9181 cgaggccgtc ttcgcccgtc acccggaggc ggtccgcgcg ggagtcccgc ttctgcccgg
     9241 cgtcggattc gacgtcgtgc cgtccgactg cctcgccgcc gcgctcgcag aggcgctccc
     9301 cggcgccgtg aggctcgagc tcgcgttccg cggcttcggg atttccggcg gcacggcccg
     9361 gacgatgctg gaggggctgc cgaagggcgg ggcggcgcgc gtcgacggga agctcgtccc
     9421 ggtgccgccg gcgtggaaga cgcggaccgt cccgttctcc gaccggccgc ggctcgcggt
     9481 gacgatcccg tggggcgacc tctcgaccgc ctaccggtcg acgggcattc cgaacatcga
     9541 gacctacatc gccatgccgc ccgcgcgggt cgcggccctg cggtgggcgg gtcctctcct
     9601 ccccctcgcc gggctgctgc ccctgcagcg cgttgtcggc ccctgggtga ggaacgccgt
     9661 ttccggcccg tccgccgagg agcgggcccg ggagcgctcg ctcctctggg ggcgcgtgga
     9721 ggacggggag gggcgctcgg tcgaagggac cgtcgagacg ctcgagggct acgcgctgac
     9781 cgccgagacc gccaccctcg cggtggaaag ggtcctccgc cgttcggtgg agccgggagc
     9841 cttcacgccg tcgagagcgt tcggggcccg gtgcatcgag aaggtccgcg acacgaagat
     9901 gtccgtaccc gcgcgggcgg ccgacgcggc gccccgcagg gcctcgcgga aggagtgacg
     9961 cgccgtccta ccagaggagg aagtcgagcg tctcgcccgc cgcctcgtgg taggtcccct
    10021 cgatgcggaa cgcgccgtcg gcgtcgttca ggacggcgta ctcgggcacg gacttcccgg
    10081 agtacgagag ctcgtagagc aggtggagcg tgcgggtccg cgtgaagagg ttccggctca
    10141 cccgcgggcg cagcagcttc gcgccggcga gcgcccccgt ctcggcccgc cgcttctcgt
    10201 gcgctgcgac gaatttctcg cgcgggacct cgtcacggta gctcttcgtg aggcaacgct
    10261 cccacgcctc ggcgtacttc ccctcgccga cgaggcggac gtactcggcg acgacggtcc
    10321 gatcggcgtc ccggacgatc gcgtcgtccg agaccgtccc gcgccgcgcg agccggaaga
    10381 ccacggcgcc gacggcgacg aggagcagga ggacccagag gccggacgac ggtgttccct
    10441 tcattgctcc tcccggccgg ccccgtcggt ggcccttgcg gctccggggc cgcgcccgcc
    10501 tcccgcgcga agggggccgc cggtcatcgt acggcgaacg ggccttcgcg ggcgggccgc
    10561 ggccgtcgca gaatcggccc gcccggtccg agcggccggc gaggaggttc ccgatggccc
    10621 gtcccgatcc ccatccgccc gaggtcttcg aacggctccg ccggcgcggg cccgaaacgc
    10681 ggatcgccgt cgtcggcgcg agcaacgccc cggagaagta cgggaacgtc atcgtccgga
    10741 acctggccgc gaaggggtac accgtcctcc ccgtcaaccc ccgcgagacc gagatcgccg
    10801 gcctccccgc ctggccgaac ctcgaatcgg tccccggccc cgtccacatc gtgaacgtcg
    10861 tcgtcccgcc gcaggtgacc ctcggggtcc tcgcgaagtc ggcggagctc ggcctgccgg
    10921 ccgtctggct ccaggacggg agcttcgacg acgcggtcct cgaggcggcc gcgaaggcgc
    10981 cgttcgagac cgtctacgac gcctgcatca tggtggcgag caacttcggc tgacccgggc
    11041 gatacacttc gcgccgtgag cggagagacc gagtcgatcg aaacggacgg gttccgggaa
    11101 gaatgcggcg tcttcgggat ctacgggcac cccgacgccg cgaacctcgc ctacctcggc
    11161 ctctacgccc tccagcaccg cggccaggag gcgggaggca tcgcggcctg gaacggccgg
    11221 aggatcgccg tcgagaaggg gatgggctac gtcgccgacc tcttcgacga ggcgcgcctg
    11281 gcgcgcctgc cgggaaggtc cgcggtcggg cacgtccgct actcgaccgc gggggcctcg
    11341 ctcctcgaga acgcccagcc gatcgtctac aacacgaaca agggcccgct cgcgatcgcg
    11401 cacaacggga acctcgtgaa cgccgaggag ctgcgcgcgg agctcgagca ggaggggtcg
    11461 atcttcacga cctcctccga cacggaggtc ttcctccacc tcatggcccg gtcgcgggcc
    11521 gagagcctcg tcgacgccct ccgcgacgcg ctcggccgcg cccgcggcgc ctactcgatc
    11581 gtcctcctcg ccggggagaa ggtcctcgcc gcgcgcgacc cgcagggctt ccgcccgctc
    11641 acgatcgggc gcctcggcga cgcgtgggtc gtcgcctccg agacctgcgc gttcgacctg
    11701 atcgacgccg agccggtccg cgacgtggag ccgggggaga tcgtcatcct cgacgcgggc
    11761 ggcgtcgcct cggcctcgtt cgcgatgggc cggcgcaccg cgttctgcgt cttcgagcac
    11821 gtctacttcg cccgccccga ctccaacgtc ttcagccgct ccgtcgccga gtcgcggcgc
    11881 gcgttcgggc ggaggctcgc ccgggagcac gcggtcgccg ccgacgtcgt cgtcccggtg
    11941 cccgactcgg gcatcttcgc ggcgctcggc tacgcggagg agagcggcat cccctacggc
    12001 atggggctcg tccggaacca ctacgtcggc cggaccttca tcgagccgaa gcagtcgatc
    12061 cggagcttcg gcgtgaaagt gaagctgaac cccgtgaagt cggtcgtcgg cgggaagcgc
    12121 gtcgtcctcg tcgacgactc gatcgtccgg gggaccacgt cgaagaagct cgtgcggctc
    12181 ctgaagtcgg ccggggcgac cgaggtccac atgcggatct cctccccccc gacgacccac
    12241 ccctgccact acgggatcga caccccccgc cgcaaggagc tgatcgccgc gacccacgcg
    12301 atggaggaga tccggcgctt cgtcgaagcc gactccctcg gctacctctc gctcgagggg
    12361 atgctcggcg ccttcggcaa gggagagggc gaggcctgcg ccgcctgctt ctccgggcgc
    12421 taccccgtcc cgttcgcggc cgccctggag cagcagccgc tcttcggaga ggacgaggtc
    12481 ctcccctctt cgggaagccc cgagaagcgc tgataccatc gcgccgcccg agggacgtcc
    12541 gacgggccga tcccctccga ggaggcgcgc cattccgaac gacacgccgc tcacgtacgc
    12601 cgcctccggc gtcgacatcg acgcgaagat gtccgcggtc gcccgcgcga aggaggcgat
    12661 ccgctccacc ttcacctccg gcgtcgtcgg cgacgtcggc gggttcggcg gcctcttccg
    12721 gcccgacttc cgcgggatgc aggacccgct tctcgtggcc tcggcggacg gcgtcgggac
    12781 gaagatccgg gtcgccatcg cggccgacgt ccacggcacg gtcgggcagg acatcgtcaa
    12841 ccactgcgtg aacgacatcc tcgtccaggg ggcgcgcccg ctcttcttcc tcgactacgt
    12901 ggcgacgggg cggatgcggc gcgaggtgat cgcggaggtc atcggcggcg tcgccgtcgc
    12961 ctgccgcgag aacggctgcg cgctcctcgg gggcgagacg gccgagatgc cggggatgta
    13021 cgccgacggc gactacgacc tcgccgggac gatcgtcggc gtcgtcgacc ggccgaagct
    13081 cctcgacggc tcgcgcgtcg ccgagggaga cctcctcctc gggctcccgt cgtcgggcct
    13141 ccacacgaac ggctactccc tcgcgcgcat gctcttcttc gagcgcctcg gcctcggccc
    13201 gcacgacgcc gttcccgagc tcgggacgac gatcgcggag gcgctcctcg ccgtccaccg
    13261 cagctatctc gcggccctcc tgccgctcgt cgaggacggg ctcctctccg cgatgtccca
    13321 cgtcaccggc ggcggcttcc gcgacaacct tccgcgcgtc ctgcccgagg ggctccgggc
    13381 cgtcgtcacg ccgggggcgt gggcgtggcc cgccctcttc cgcttcatcc gcgagaaggg
    13441 gagcgtgccg gaggaggaga tgctccgcgt cttcaactgc ggtgtcggga tggtcctctt
    13501 cgtcgcgccg gagcgcgcgg ccgaggtgcg gcggcggctc gcggcgtcgg gagagacggt
    13561 cctcgacgtc gggcgcgtcg agaagggccc tcggagcgtc cgcttcgcat gacggcggtg
    13621 ggcgtctcct tcgaagcgcc cgttccctgg ttccctctcc cggcgcgcct cgccgtcctc
    13681 ctctcgggcc gcggatcgaa cttcgtggcg ctcgcggacg cctgcgagtc cggcgcgctg
    13741 ccggcgcgga tcgtcctcgt cctctccgac cgggccgatg ccgcgggcct cgccagggcc
    13801 cgcgcgcgcg gactcgaggc ggcgctcgag gagcggggcg gggagacgcg cgccgcgcac
    13861 gaggcacgtc tcgccgcacg gatcgagacg gcgggcgccg acgtcgtctg cctcgccgga
    13921 ttcatgcgtg tcctctcgcc ggccttcgtc gcgcgcttcg agcgtcgcgt cgtcaacgtc
    13981 cacccgtcgc tcctgccggc cttcccgggc ctggaggccc agcatcaggc cttcgagcac
    14041 ggcgtgaagg tcgcgggcgc gacggtccac ctcgtcgacg ccgggaccga cacgggcccg
    14101 atcgccggcc aggaggccgt ggccgtcggg cccggcgacg acgcggagcg cctcgccgcc
    14161 cgcatcctcg aggtggagca tcgcctctat ccggccacgc tcgcggcgct cctcgccggg
    14221 ggctggtcga tcgagggccg ccgtctccgc ttttcgggcc ccggttccgt cgaagcgtga
    14281 cggcacgcac gctcttcgag acgaaatccc actttcgtgg gattgcgacg ctcgatcgac
    14341 aggcttagcc tccctgcaag caaacagacg gaaaggtgcg gcggtgggaa cctgttgacc
    14401 cggggagggt taggctccgc ccggcgcacg ggaaaacgct ctctcctcgc gccggagacg
    14461 gtccacggtc ctcgacaccg ggtcgccctc acccggtgtc atccccccag gggagcgggc
    14521 cgtccgatct ccggcgcgcc ttttttcgat tccacgaaga ttcacgcttg acatcccggg
    14581 gcaacttccg tatcttcccc tcccccggcg atgcccgggc ccgatctttc cagcgagcaa
    14641 aacccgagaa aatccgagag cgctttctga caggagcccg cctccgggcg gccccgaaaa
    14701 aaagctcttg acttcaaggg tcgcctgcga gagactggga atccgccgct ggacggcacc
    14761 ggttccttga aaactgaaca gagacgaacc cgtgagggtg ggcgcaagtc cagtcctcat
    14821 gggggtttac gtgattcctt tgagcggctc gtccggcggg acgagcccgg gttttggcac
    14881 cgctggtgcc gaaaccttca gtaaaacgca catctgcgcg aggccttcgg gccgagcgtt
    14941 gagacaagtt tcaaactttt tttggagagt ttgatcctgg ctcagagtga acgctggcgg
    15001 cgtgcctaac acatgcaagt cg
//
LOCUS       DAOTEZ010000054        14881 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2769_length_14881_cov_5.411304, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000054 DAOTEZ010000000
VERSION     DAOTEZ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14881
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2769_length_14881_cov_5.411304"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1502
                     /locus_tag="P5164_04425"
     CDS             <1..1502
                     /locus_tag="P5164_04425"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRY43173.1"
                     /translation="DDGREAGGDDARGAPAEGVRAVRGATVRTALVAAFLLALSAAGA
                     PAAKSAAKKAPCDLVVVAGHALPMDAAFTVRGATAVAVRGGAIVAVGDPAEVDAAWAP
                     KQRIERKNAVLLPGLVNTHGHAAMNLLRGISNDQPLMEWLTKYIFPAEAKNVSPAFVT
                     AGTELACLEMIRGGTTTFTDMYYYESDVAAVVDRAGLRGVLGETWIDFPAPGHANLEE
                     TKTVTRAFLEKWKGHPRITPAIAPHAPYTCSRETLQAARALADEYGAPLLTHVSETWD
                     EQMQVREKHGKSPTAWLDEVGFLGPRLCVAHGVWLSAEDLATLAARGVSLAHNPESNM
                     KLGSGIAPVAAARKAGVVVGLGTDGSAGSNNDLDMFDAMDFAGKLAKVAALDPTPLTA
                     PELLRMATIEGAKALGLDRLVGSIETGKKADLIVVDLGKAHVEPVFDLPSTVVYASRA
                     GDVSLTVVDGKVLWDGKRVTTLDEAKVLAAAREWRGKVLKSLETSAEKK"
     gene            1499..2809
                     /locus_tag="P5164_04430"
     CDS             1499..2809
                     /locus_tag="P5164_04430"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HRY43174.1"
                     /translation="MSLIVRTIVKKRDGGELSEGEIRDFVAGVTSRTVTDEQAAALLM
                     AICCRGMSTAETAALTFAMMNSGETWDLSPHGFVADKHSTGGVGDKSTLVLAPLLAAC
                     GVKTGMMSGRGLGHTGGTLDKLEAIPGFVTGQSREAFDRLVETVGCALIAATDTVAPA
                     DRRLYALRDVTGTVESIPLITASILSKKLATGAHAVVFDVKTGNGAFMQKPEDAHTLG
                     RALVDTTRATGRKASGYVTAMDRPVGVAVGNANEVEESIRLLRGDGPADLRENVLLLG
                     ADLLVAAGVADTEKEARRRLNAALQDGTALETFARVISEQGGNPAVCDDLSLLPQPVA
                     KHEVKAAAPGTIRRVATRELGLLSIEIGCGRARKEDVVDPASGFRILKKTGEDVAEGE
                     PLAVVELGPRTTLPKAYLDRLGACWEIGASAGAEPPLPFVVEKL"
     gene            complement(2859..3857)
                     /locus_tag="P5164_04435"
     CDS             complement(2859..3857)
                     /locus_tag="P5164_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRY43175.1"
                     /translation="MAFAWLSGSDDVAELIAKRNYGRAAKVLRGLLAKDPSNASLKQQ
                     LGDVLALDGKSYEAVELLWKLADDYATSGFVGKAIAVLKKVQRIDPSLTKVEEKLARL
                     VRQKDDESTLALRVRAGAMRRRTELETTPTPSPEPPVAKPPAAEPFVLDLETDDGAPP
                     EGAAETAPRSLHPVDEEAAQGMVASPLFSDFSSEELVEVIKGLELLTYEAGDVIVAEG
                     APGDSLFVLTTGTVKAFVKDPDGHYHKVREMYEGAFFGEVSILTGKPRSATITAATPC
                     ELLALDRSALESIAERQPNVMTILRHFCEARYGSVDEQQIRRGDTHPGVSRRTSAG"
     gene            3941..4309
                     /locus_tag="P5164_04440"
     CDS             3941..4309
                     /locus_tag="P5164_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019508830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY43176.1"
                     /translation="MAKILVVDDDEMNRDMLSRRLLRKGYEVSLAVNGAEAIEKARSE
                     RPDLILMDLSMPVLDGYEATRRLKAEGATSRIPIIGLSAHAMVGDREKALSAGCDDYD
                     TKPVELPRLLGKVESSLGAG"
     gene            complement(4316..6151)
                     /locus_tag="P5164_04445"
     CDS             complement(4316..6151)
                     /locus_tag="P5164_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY43177.1"
                     /translation="MSDEDLLEKAFAAEEAAPRLASGNRVVPPQLLSKLRHDMRTPIH
                     QILGYAEMVEEDAIATGQTGCAADLGKIQDAARRLLEMVDTLQERLLPATAEPVPPEP
                     EPLPAPDASRMPPGEAPALTGPIRRADVEIPVGERVEPAHLLVVDDNAENREMLSRRL
                     QAHGFTIAVAENGPEALRLLEAVPFDLVLLDVVMPGMSGLEVLRDLRGRHDAADLPVI
                     MATARDDSNDVVTALRLGANDYVTKPLDFPVVLARVGTQLSLARAKARIGTLAQDLEV
                     RNRFIFETFGRYLSNEVVERLLQTPEGLKLGGETRKVTLLMSDLRGFTQVADRIPPDR
                     VVKLLNNYLGTMADLLLSFQGTIDEFIGDAILAIFGAPVQREDDALRAVACAVEMQKA
                     MERVNEFNRREGLPSVEMGIAIHTGEVVVGNIGSDKRAKYGVVGSPVNLTARIESYTV
                     GGQILVSDRTRREGGGQLSVGSEVVVQMKGFENPVSAWDVRGVAGGFDLFLPARDEPL
                     HALRGPLAVRFATISGKRVDGPESEGLLVRLSMKEAEVETRERPDAFRNLRLRFVGND
                     GRTIPGELYGKVFTPRAGTKGFGLRFTSVPPEIRSFLETVLSGSR"
     gene            6240..7136
                     /locus_tag="P5164_04450"
     CDS             6240..7136
                     /locus_tag="P5164_04450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRY43178.1"
                     /translation="MNELYQELLPLGREGVVPAGDELWQEGAKGDNVVLLVEGTFEVL
                     QSSPSGEPIVLRLARAGDVLGEMACLDGGARSAGLRAATDCRIRTIPAEEFRDFIRLR
                     PHLMEELFRIESQRIRSLSRMVSQSQHRAITDRLTSLYNYGFFIERLDLELERAVQMR
                     DAVSVVLFDIDHFKAYNDTHGHPAGNVVLVQVSDLLRRTARRGDIVARFGGEEFVVLL
                     YGASRREALQVAEAARARVEATAFAGEETQPLGRVTISGGVACFPSDARGATDLLKAA
                     DVRLYKAKGAGRNRVESGGDAA"
     gene            7202..9145
                     /locus_tag="P5164_04455"
     CDS             7202..9145
                     /locus_tag="P5164_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRY43179.1"
                     /translation="MLYRLERVHKSFGTKTVLTGATWQHDPGRVVGLVGRNGAGKSTL
                     LRIVPGQIEPDEGRVLLAGGTTFASLEQAVEPEGDEPLRDFVARAQEGLRGLEGEMRR
                     LEEAIASASASGAGEAVEPLLAEHDAIRERFERNGGWEADARVERVLLGVGLPRETWD
                     RPVGELSGGQKHRAQIARLLLGEASVLLLDEPTNHLDLAGLEFLETWLVERKAAAAQA
                     ALVVSHDRRFLNAVADRIVEVSHGQLEEYPGDYEAYRRLKAERDRVRAKAAEEQRKLV
                     ERTEDFIRRNIAGQKTKQAQARRKMLAKLERVEAPREDANEVTFRFEEARVSGDRVLE
                     LKGLVPGYPETGPLLSPVSFLLRRGERIALWGPNGCGKTTLLKTVARLLPPIAGSAVP
                     GTGVIVGYYDQEMRDLPSSWTVLDVVRELDPTLLDGEARDFLALFDFRGDEVEQRVAT
                     LSGGEKGRLSLARIVFGGANLLLLDEPTNHLDLDSRERLEEALLDFEGTIVFVSHDRW
                     FVDRIATHVLEVKERQAALWPGSWSDLVRRRAAEKAAAEETATAPARIPSERSPARRP
                     DRQAERQRAKARREVESLEEEVARLEERVREIDAALADPAVWKDGPRTRELTLARDDA
                     RRAVDASYAAWEEAVKKLEGVPA"
     gene            9142..10398
                     /locus_tag="P5164_04460"
     CDS             9142..10398
                     /locus_tag="P5164_04460"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HRY43180.1"
                     /translation="MTETASILAVGSELLGTTRLDTNSLFLTGELEAIGVRVVRKACV
                     VDGWDELLAELRFALERAPLLVVTGGLGPTEDDRTKEAVAHLLGRRLLRDEEILATLK
                     ERFRKRGFEMPAVNAKQADVIEGAFVLSNRRGTAPGYVVEDGGRTIVLLPGVPHEMKA
                     LIVEAVLPRLTGGAPREGLHRRVLKVVGLGESMVEQLVKPVYEAHREHDVTILSAAPG
                     EVQLHFAARGGCAEAGRTLDVLEAAFRAALGGAFFGRDEETLEGVVGRLLREAGQTLA
                     LAESCTGGMIAARLTDVAGSSDVFPGGVVAYANEAKVALLGVSPESIARWGAVSEQVA
                     REMAAGARRRFGASAGLAVTGVAGPSGGTPEKPVGTVHLALDAEAGIRHERLTLAGDR
                     AMIRRWTTSAALGLVRAWLTEGGGAK"
     gene            10395..11396
                     /locus_tag="P5164_04465"
     CDS             10395..11396
                     /locus_tag="P5164_04465"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRY43181.1"
                     /translation="MKKIAIVGTGAWGTALAIHGARAGLAVSLLGYRDDIVDDLARTR
                     AHPALPGAEPLPALVQPTMSPDEAFDGAETVLWSVSVQFTASELERLGPWLRRGLPLA
                     STSKGIEIGTHRRTSEMMVDALGPDVPVAVLSGPTFAIEVARGLPTVAVVACADAAVA
                     ERLQKGLASPTFRLYRSSDIVGVEIAGATKNVVAIAAGIVDGLKLGHNTRAALLTRAL
                     AEIRRLGTRLGGQPETFSGLAGVGDLILTATGDLSRNRRVGLALASGLSVDEALASLG
                     GQVAEGVPTAGAIVELARTVGVEMPISETVRSILEGTTAPREAVSRLMTRSLKEEGA"
     gene            11456..12361
                     /locus_tag="P5164_04470"
     CDS             11456..12361
                     /locus_tag="P5164_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43182.1"
                     /translation="MAVLDMNHGFPNLGHASVVGRLERIGAEERERLGGGAPEVRVVS
                     YDVRRGLAVPSSADRFPLVVGTGGPGALDPRENDGISPLSQGIDEDPAWEAPLWRFFD
                     ALLGRSDGALFGICHTFGLLARWSGFGEAVARGPEKGKSAGAVTNRLTPEAEAHLWFG
                     GYYRTSSGGRVRVLDSRLFDILPSGRGPGTILAWESNDAGERGDAATMVELDRYADGA
                     PRVWAVNHHPEIGGAAEQRERLLRLASEREVGPGWLEERLRALDAFDVSPEVEKELRA
                     TRAFTFEEPVRAVLARALAERSGTA"
     gene            complement(12368..12679)
                     /locus_tag="P5164_04475"
     CDS             complement(12368..12679)
                     /locus_tag="P5164_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008122832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43183.1"
                     /translation="MEHRDGHPHEHGPGCGHRAIRHGDHADYLHDGHLHSPGESGVAE
                     HVLAVDHGHPAACAPVECPARHHPGCPHPRVPHGDHFDYLVDGALHHLHGDHCDLHGT
                     V"
     gene            12751..13845
                     /locus_tag="P5164_04480"
     CDS             12751..13845
                     /locus_tag="P5164_04480"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HRY43184.1"
                     /translation="MTRARVRLAIGLSLAAAGCAPSSSRPAAGPQVAVATIAPLADLV
                     RRVAGPEWTVRTVIRPGTSPHVFEPEPSDLRKLLGARAVFVAGAGYDDWMRKVLAACA
                     SRAPVHDAAASIGIGPGRAWSASAGRDLPAQGPEAHGEDDLVHAAGPHDRDGHAAHDH
                     EAAGHEGHGHEDDPGGAAGAHDGHAHGILGEDPHWWLSPELAARSLGGIAEAFAAVDP
                     IHAESYRARAREAAAELVELDGEISAKLAPVRGKRFVSAHRAWVYFADRYGLVEAASI
                     EPVPGREPSPRELKALVTEGRQGGLGRLFTEPQFPPAAARVVANEAGLDLTLVDPIGG
                     VPGRSTYPELMRFNADAFLRGLSGAAERRP"
     gene            13842..14501
                     /locus_tag="P5164_04485"
     CDS             13842..14501
                     /locus_tag="P5164_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY43185.1"
                     /translation="MSVAACFENVTVRFGGRPAVESTSFTIPAGMRTAMVGPNGGGKT
                     TLARVLLGILRPDAGRVAFLDEQGRELPRPRIGYLPQRSTLSPQAPVTGLDLVGLSLA
                     PGPGFGMSPAVEAEARRALGRAGLADDLATRPVGRLSGGQRQRVLLARAIAGTPAILL
                     LDEPDTALDAEGMKTLRRLVAEEVAAGRTIIYVTHDSDAMGGADAVFRIDGRLTEETR
                     A"
     gene            14498..>14881
                     /locus_tag="P5164_04490"
     CDS             14498..>14881
                     /locus_tag="P5164_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRY43186.1"
                     /translation="MTEFLAFPFFQRALLAGFVVAVVCGALSFFVVLRRLAFVGSGVS
                     HAAFGGVALAALVGAPPGLGALLAALAVAFATAKASGDGDLTEDTAVGVFTVAGMAVG
                     VVAISFLETNVDLFGLMFGNILTVDP"
BASE COUNT         2017 a         5274 c         5554 g         2036 t
ORIGIN      
        1 gcgacgacgg gcgggaagct ggaggagacg acgctcgcgg cgctcctgcc gagggcgttc
       61 gagctgtgag gggcgcgacc gtacggaccg cgctcgtcgc cgcgttcctc ctcgccctct
      121 ccgccgcggg cgctcccgcc gcgaagtcgg ccgcgaagaa ggccccgtgc gacctcgtcg
      181 tcgtcgcggg gcacgccctc ccgatggacg cggccttcac cgtgcgggga gcgacggccg
      241 tcgccgtccg gggcggcgcg atcgtcgccg tcggcgaccc ggcggaggtc gacgccgcgt
      301 gggcgccgaa gcagcggatc gagcggaaga acgccgttct cctcccggga ctcgtgaaca
      361 cgcacggaca cgccgcgatg aacctcctgc gcggcatctc gaacgaccag ccgctcatgg
      421 agtggctcac gaagtacatc ttcccggccg aggcgaagaa cgtctcgccg gcgttcgtga
      481 cggccgggac ggagctcgcc tgcctcgaga tgatccgggg cgggacgacg acgttcaccg
      541 acatgtacta ctacgagtcg gacgtggcgg ccgttgtcga ccgcgccggc ctccggggcg
      601 tcctcggcga gacctggatc gacttcccgg cgcccggcca cgcgaacctc gaggagacga
      661 agacggtcac gcgcgccttc ctcgagaagt ggaaggggca cccgcggatc actccggcga
      721 tcgcgccgca cgccccctac acgtgcagcc gggagacgct tcaggcggcc cgggcgctcg
      781 ccgacgagta cggcgccccg ctcctgacgc acgtctcgga gacctgggac gagcagatgc
      841 aggtccgcga gaagcacggg aagagtccga ccgcctggct cgacgaggtc gggttcctcg
      901 ggccccggct ctgcgtcgcg cacggcgtct ggctctcggc cgaagacctt gcgaccctcg
      961 ccgcgcgggg cgtgagcctg gcgcacaacc ccgagtcgaa catgaagctc ggctcgggga
     1021 tcgcccctgt cgcggcggcc cggaaggccg gcgtcgtcgt cggcctcggg accgacggct
     1081 cggccggctc gaacaacgac ctcgacatgt tcgacgcgat ggacttcgcg gggaagctcg
     1141 cgaaggtcgc cgctctcgac ccgacgccgc tcacggcccc ggagctcctc cggatggcga
     1201 cgatcgaggg agcgaaggcg ctcggcctcg accgcctcgt cgggtcgatc gagacgggga
     1261 agaaggccga cctgatcgtc gtggacctcg gaaaggccca cgtggagccc gttttcgacc
     1321 tcccctccac cgtcgtctac gcctcccgcg cgggagacgt ctcgctcacc gtcgtcgacg
     1381 ggaaggtcct ctgggacggg aagcgggtca cgacgctcga cgaggcgaag gtcctcgccg
     1441 cggcgcggga gtggcggggg aaggtcctga agtcgctcga gacgagcgcg gagaagaaat
     1501 gagcctcatc gttcgaacca tcgtcaagaa gcgcgacggc ggcgagctgt cggaagggga
     1561 gatccgggac ttcgtcgcgg gggtcacgag ccgcacggtc accgacgagc aggcggcggc
     1621 cctcctgatg gcgatctgct gccgcggcat gtcgaccgcc gagacggcgg ccctcacgtt
     1681 cgccatgatg aactcgggcg agacgtggga cctctcgccc cacggcttcg tcgccgacaa
     1741 gcactcgacg ggaggcgtcg gcgacaagtc gaccctcgtc ctcgcgccgc tcctcgcggc
     1801 ctgcggggtg aagacgggaa tgatgtcggg acggggcctc ggccacaccg gcggcacgct
     1861 cgacaagctc gaggcgatcc ccggcttcgt gacggggcag tcgagggagg ccttcgaccg
     1921 gctcgtcgag acggtcggct gcgccctcat cgccgcgacg gacaccgtcg cgccggccga
     1981 ccggcgcctc tacgcgctcc gcgacgtgac ggggacggtc gagtcgatcc cgctcatcac
     2041 ggcctcgatc ctctcgaaga agctcgccac gggggcgcac gccgtcgtct tcgacgtgaa
     2101 gacggggaac ggtgccttca tgcagaagcc cgaggacgcc cacacgctcg gccgggcgct
     2161 cgtcgacacg acccgcgcca ccgggcgcaa ggcgagcggc tacgtgaccg ccatggaccg
     2221 ccccgtcggc gtggccgtcg ggaacgcgaa cgaggtcgag gagtcgatcc gccttctccg
     2281 gggggacggg ccggccgacc tcagggagaa cgtcctcctc ctcggcgcgg acctcctcgt
     2341 cgccgcggga gtcgcggaca cggagaagga ggcgcgacgg cgcctgaacg ccgccctcca
     2401 ggacggaacg gccctcgaga cgttcgcccg cgtcatctcc gagcagggcg ggaacccggc
     2461 cgtctgcgac gacctctcgc tcctcccgca gcccgtcgcg aagcacgagg tgaaggccgc
     2521 ggcccccggg accatccggc gtgtcgcgac gcgcgagctc gggctcctgt cgatcgagat
     2581 cggctgcggg cgcgcccgca aggaggacgt cgtcgacccg gcgtccgggt tccggatcct
     2641 gaagaagacg ggcgaggacg tggcagaggg ggagcccctc gccgtcgtcg agctcggccc
     2701 gcggaccacg ctcccgaagg cctatctcga caggctcggg gcctgctggg agatcggggc
     2761 gtccgccggc gccgagccgc cgctgccgtt cgtcgtcgag aagctctgac cccgcgcccg
     2821 cccggcgtcc ccggtccgca ccgcgggacg ccggcgcgtc agccggccga cgtgcggcgg
     2881 ctcacgccgg ggtgggtgtc gccccggcgg atctgctgct cgtcgacgct tccgtaccgg
     2941 gcctcgcaga aatgccggag gatcgtcatg acgttcggct ggcgctccgc gatcgactcc
     3001 agggccgaac ggtcgagcgc gagcagctcg cacggcgtcg ccgccgtgat cgtcgcgctg
     3061 cggggctttc cggtcaggat cgagacctcg ccgaagaagg ccccctcgta catctcgcgg
     3121 accttgtggt agtgcccgtc ggggtccttc acgaaagcct tgaccgttcc cgtcgtgagg
     3181 acgaagaggg agtctcccgg cgctccctcg gcgacgatga cgtctcccgc ctcgtaggtg
     3241 aggagctcga gacccttgat gacctccacg agctcctcgc tcgagaagtc gctgaacagg
     3301 gggctcgcga ccatcccctg ggccgcttcc tcgtcgacgg ggtggaggct gcgcggcgcc
     3361 gtctcggcgg cgccctccgg aggcgcgccg tcgtcggtct cgaggtcgag gacgaacggc
     3421 tcggcggccg gcggcttcgc gaccggaggc tccggcgagg gcgtcggggt ggtctccagc
     3481 tcggtgcgcc gccgcatggc gcccgcccgg acgcgcaggg cgagcgtcga ctcgtcgtcc
     3541 ttctggcgca cgaggcgggc cagcttctcc tcgaccttcg tgaggctcgg gtcgatccgc
     3601 tggaccttct tgaggacggc gatcgccttg ccgacgaaac ccgaggtcgc gtagtcgtcg
     3661 gcgagcttcc acaggagctc gacggcttcg tagctcttcc cgtccagggc caggacgtcc
     3721 ccgagctgct gcttcaggga agcgttcgaa gggtccttgg cgaggagtcc gcggaggacc
     3781 ttcgccgcgc ggccgtagtt gcgcttcgcg atcaactcgg cgacgtcgtc agagccggag
     3841 agccacgcga acgccattcg attctcgctc cgagggcgac ggtagggcac cgttccggct
     3901 gctgtcaagg cggcagagcg ctacactccg ggcggcaccg atggcgaaga tcctggtcgt
     3961 cgacgacgac gagatgaacc gcgacatgct ctcgcgccgc cttctccgga aggggtacga
     4021 ggtgtctctc gccgtgaacg gggcggaggc gatcgagaag gcccgctcgg agaggccaga
     4081 cctcatcctg atggacctct cgatgccggt cctcgacggc tacgaggcga cgcggaggct
     4141 gaaggcggaa ggggcgacct cgcgcatccc gatcatcggc ctctcggcgc acgcgatggt
     4201 cggagaccgc gagaaggcgc tctcggcggg gtgcgacgac tacgacacga agccggtgga
     4261 gctcccgagg ctcctcggca aggtcgagtc gtccctcggg gcgggctgag ggacctcagc
     4321 ggctgccgga gaggaccgtc tccaggaagg agcggatctc cggcgggacc gacgtgaacc
     4381 ggagcccgaa ccccttcgtc ccggccctcg gcgtgaagac cttgccgtag agctcacccg
     4441 ggatcgtcct tccgtcgttt ccgacgaacc ggaggcggag gttgcggaac gcgtcggggc
     4501 gttcgcgcgt ctcgacctcg gcctccttca tcgacaggcg aacgaggagc ccttcggact
     4561 cggggccgtc caccctcttt ccggagatcg tcgcgaaccg gaccgcgagc ggtccgcgga
     4621 gggcgtggag gggctcgtcc cgggccggga ggaagaggtc gaagcccccg gccacgccgc
     4681 ggacgtccca ggcggagacc gggttctcga accccttcat ctggacgacg acctcgctcc
     4741 cgaccgagag ctggcccccg ccctcgcgcc gggtccggtc gctgaccagg atctggccgc
     4801 cgaccgtgta cgactcgatc cgggccgtga ggttcacggg gctcccgacg acgccgtact
     4861 tcgcccgctt gtccgagccg atgttcccga cgacgacctc gccggtgtgg atcgcgatcc
     4921 ccatctcgac cgacgggagc ccctcccgcc ggttgaactc gttcactcgc tccatcgcct
     4981 tctgcatctc gacggcgcac gcgacggcgc gcagggcgtc gtcctccctc tggacgggcg
     5041 ccccgaagat cgccaggatc gcgtccccga tgaactcgtc gatcgtcccc tggaaagaca
     5101 ggaggaggtc ggccatcgtc ccgaggtagt tgttcaggag cttgacgacc cggtcggggg
     5161 ggatgcggtc cgcgacctga gtgaagccgc ggaggtcgga catcaggagc gtgaccttcc
     5221 gcgtctctcc tccgagcttc agcccctcgg gcgtctggag cagccgttcg acgacctcgt
     5281 tcgagaggta gcgcccgaac gtctcgaaga tgaaccggtt gcgcacctcg aggtcctgcg
     5341 cgagcgtgcc gatccgggcc ttggcccggg cgagggagag ctgcgtgccg acgcgggcga
     5401 ggacgacggg aaagtcgagc ggcttcgtca cgtagtcgtt ggcgccgagg cggagcgcgg
     5461 tgacgacgtc gttcgagtcg tcccgggccg tggccatgat gacgggaagg tccgcggcgt
     5521 cgtgccggcc gcggaggtcc cgcaggacct cgaggccgct cattccgggc atgacgacgt
     5581 cgaggaggac gaggtcgaag gggaccgcct cgaggaggcg aagggcttcc gggccgtttt
     5641 cggcgaccgc gatcgtgaag ccgtgcgcct ggaggcgccg ggagagcatc tcgcggttct
     5701 ccgcgttgtc gtcgacgacg aggaggtgcg ccggctcgac tctctcgccg accgggatct
     5761 cgacgtcggc gcggcggatc ggccccgtca gggcgggggc ctctcccggc ggcatgcggg
     5821 aggcgtccgg agcgggaagg ggctccggct ccgggggaac gggctcggcc gtcgcgggga
     5881 ggagcctctc ctggagcgtg tcgaccatct cgaggagccg gcgtgcggcg tcctggatct
     5941 tccccaggtc ggcggcgcaa cccgtctggc cggtggcgat ggcgtcctcc tccaccatct
     6001 ccgcgtagcc gaggatctgg tgaatgggcg tccgcatgtc gtgccgcagc ttggagagga
     6061 gctgcggcgg cacgacccgg ttccccgacg cgagccgcgg cgccgcttcc tccgcggcaa
     6121 acgctttctc gagcaggtcc tcgtccgaca tcgagcgtcg agtgtagcgc cgcccggagg
     6181 gcgggcgcgg agcgcggcga tatacttcgc ggcacggaac gcgaggagcc gggagcgccg
     6241 tgaacgagct ttaccaggag ctgctgccgc tcggacggga gggcgtcgtt cccgcggggg
     6301 acgagctctg gcaggaaggg gcgaagggcg acaacgtcgt cctcctcgtc gaggggacgt
     6361 tcgaggtcct ccagtcctct ccctccggcg agccgatcgt cctgcgcctc gcgcgcgccg
     6421 gggacgttct cggcgagatg gcctgcctcg acggcggggc ccggtcggcc ggcctccggg
     6481 ccgcgacgga ctgccgcata cggaccatcc cggcggagga gttccgtgac ttcatccgcc
     6541 tccggccgca cctgatggag gagctcttcc ggatcgagtc gcagaggata cgaagcctct
     6601 cccggatggt ctcgcagagc cagcatcgcg ccatcaccga ccggctcacc tcgctctaca
     6661 actacgggtt cttcatcgag cgcctcgacc tcgagctcga gcgcgccgtg cagatgcgcg
     6721 acgccgtcag cgtcgtcctc ttcgacatcg accacttcaa ggcctacaac gatacgcacg
     6781 gccaccccgc ggggaacgtc gtcctcgtgc aggtctccga cctcctgcgc cggaccgcac
     6841 ggaggggcga catcgtcgcc cggttcggcg gcgaggagtt cgtcgtcctt ctttacggcg
     6901 cctcccggcg cgaggcgctc caggtggcgg aggccgcccg cgcgcgggtc gaggccaccg
     6961 ccttcgcggg cgaggagacc cagcccctgg gccgcgtgac gatcagcgga ggggtcgcgt
     7021 gcttcccctc ggacgcgcgc ggcgcgaccg acctgctgaa ggcggccgac gtgcggctct
     7081 acaaggcgaa gggcgccggc cggaaccgcg tcgagtcggg cggcgacgcg gcctagccgg
     7141 gccgcggccc gcgccccggc gctccgggca cggccgggga ctcgaaggtt atcctccgcc
     7201 cgtgctctac cggctcgagc gcgtccacaa gtccttcggc acgaagacgg tcctgaccgg
     7261 cgcgacctgg cagcacgacc cggggcgggt cgtcggtctc gtgggacgga acggcgccgg
     7321 gaagtcgacc ctcctccgga tcgtcccggg acagatcgag cccgacgagg ggcgggtcct
     7381 cctcgcgggc gggacgacgt tcgcgtccct cgagcaggcc gtcgagcccg aaggcgacga
     7441 gccgctccgg gacttcgtcg cccgggcgca ggaggggctg cggggcctgg agggggagat
     7501 gcgccggctc gaggaggcga ttgcgtccgc atccgcctcg ggcgccggcg aggccgtcga
     7561 gcctctcctg gccgagcacg acgccatacg ggagcgcttc gagcggaacg gcggctggga
     7621 ggccgacgcg cgcgtcgagc gagtcctgct cggggtcggc ctgccgcggg agacgtggga
     7681 ccggcccgtc ggggagctct ccggagggca gaagcaccgg gcgcagatcg cgcgcctcct
     7741 gctcggcgag gcctccgtcc tcctcctcga cgagccgacg aaccacctcg acctcgccgg
     7801 actggagttc ctcgagacgt ggctcgtgga acggaaggcc gccgccgctc aggcggcgct
     7861 cgtcgtctcg cacgaccgcc gttttctgaa cgccgtcgcc gaccggatcg tggaggtctc
     7921 ccacgggcag ctcgaggagt acccggggga ctacgaggcc taccgcaggc tgaaggcgga
     7981 gcgggaccgc gtccgggcga aggccgccga ggagcagcgg aagctcgtcg agcggaccga
     8041 ggacttcatc cggcggaaca tcgcggggca gaagacgaag caggcccagg cgcgccggaa
     8101 gatgctcgcg aagctcgagc gcgtggaggc gccgcgcgag gacgcgaacg aggtgacgtt
     8161 ccggttcgag gaggcgcgcg tctcgggcga ccgcgtcctc gagctgaagg ggctcgttcc
     8221 cggctacccc gagacgggcc ccctcctctc tcccgtctcg ttcctcctcc ggcgcgggga
     8281 gcggatcgcc ctctgggggc cgaacggctg cgggaagacg acgctcctga agacggtcgc
     8341 gcgcctcctc ccgccgatcg ccgggagcgc cgttccgggg accggggtga tcgtcggcta
     8401 ctacgaccag gagatgcgcg acctcccgtc ctcctggacg gtcctcgacg tcgtccgcga
     8461 gctcgacccg acgcttctcg acggcgaggc gcgcgacttc ctcgcgctct tcgacttccg
     8521 gggcgacgag gtggagcagc gggtcgcgac cctctccggc ggcgagaagg ggcggctctc
     8581 gctcgcccgg atcgtcttcg gcggcgcgaa cctcctcctc ctcgacgagc cgacgaacca
     8641 cctcgacctc gactcgcgcg agcggctcga ggaggcgctc ctcgacttcg aggggacgat
     8701 cgtcttcgtc tcgcacgacc gctggttcgt cgaccggatc gcgacccacg tcctcgaggt
     8761 gaaggagcgc caggccgcgc tctggccggg gagctggtcc gacctcgtga ggaggcgggc
     8821 ggccgagaaa gccgctgccg aggagacggc gacggccccc gcacgcatcc cgtcggagcg
     8881 gtcgccggcg cggcgccccg accgccaggc cgagcggcag cgggcgaagg cccggcggga
     8941 ggtcgaatcg ctcgaggagg aggtcgcgcg gctggaggag cgggtccgcg agatcgacgc
     9001 ggcgctggcc gacccggccg tctggaagga cgggccgcgg acccgcgagc tgaccctcgc
     9061 gcgcgacgac gcgcggcggg cggtcgacgc gagctacgcg gcgtgggagg aagcggtgaa
     9121 gaagctcgaa ggggtgcccg cgtgacggag accgcctcga tcctggccgt cggaagcgag
     9181 ctcctcggga cgacccggct cgacacgaac tctctcttcc tgacggggga gctcgaggcg
     9241 atcggcgtgc gcgtcgtccg gaaggcgtgc gtggtcgacg gctgggacga gctcctcgcc
     9301 gagcttcgct tcgccctcga gcgggcgccg ctcctcgtcg tgacgggcgg gctcgggccg
     9361 accgaggacg accggacgaa ggaggccgtg gcgcacctgc tcgggcgccg tctcctccgc
     9421 gacgaagaga tcctcgcgac cctgaaggag cggttccgga agcgcggctt cgagatgccc
     9481 gcggtgaacg cgaagcaggc cgacgtgatc gagggggcgt tcgtcctctc gaaccggcgg
     9541 gggacggcgc ccggctacgt cgtcgaggac ggggggcgga cgatcgtcct cctgccgggc
     9601 gtcccccacg agatgaaggc gctcatcgtc gaggcggtcc tgccgcgcct gacgggaggc
     9661 gcgccccggg aggggctcca tcgtcgagtc ctgaaggtcg tcggcctcgg cgagagcatg
     9721 gtcgagcagc tcgtgaagcc ggtctacgag gcccatcggg agcacgacgt cacgatcctc
     9781 tccgcggcgc ccggggaggt ccagctccac ttcgccgcgc gcggcggctg cgcggaggcc
     9841 gggcggacgc tcgacgtcct cgaggctgcg tttcgcgccg cgctcggggg ggctttcttc
     9901 ggccgggacg aggagacgct cgaaggcgtc gtcggccgcc tgctgcgcga ggccgggcaa
     9961 acgctcgccc tcgccgagtc gtgcaccgga gggatgatcg cggcccgcct cacggacgtc
    10021 gcggggtcgt cggacgtctt cccgggaggc gtcgtcgcct acgcgaacga agcgaaggtg
    10081 gcgctcctgg gcgtctcgcc ggagtcgatc gcacgctggg gggcggtctc ggagcaggtc
    10141 gcccgggaga tggccgccgg cgcgaggcgc cgcttcggcg cctcggcggg gctcgccgtc
    10201 acgggcgtcg ccggtccctc gggcgggacg cccgagaagc cggtcgggac ggtccacctc
    10261 gcgctcgacg cggaggcggg gatccggcac gaacggctga cgctcgccgg ggatcgcgcg
    10321 atgatccggc gctggaccac ctcggcggcg ctcgggctgg tccgggcctg gctgaccgaa
    10381 ggaggggggg cgaagtgaag aagatcgcga tcgtcgggac gggtgcctgg ggaacggccc
    10441 tcgccattca cggtgcgcgg gccgggctgg ccgtcagcct cctcggctac cgcgacgaca
    10501 tcgtcgacga cctggctcgg acgcgcgccc accccgcgct gcccggcgcc gagccgctcc
    10561 cggccctcgt ccagccgacg atgagcccgg acgaggcctt cgacggcgcc gagacggtcc
    10621 tctggtccgt ctcggtccag ttcacggcga gcgagctgga gcggctcggc ccgtggctgc
    10681 gccgcggact gccgctcgct tcgacctcga aggggatcga gatcgggacc caccgccgga
    10741 cgagcgagat gatggtcgac gcgctcgggc ccgacgtccc cgtcgccgtg ctctccggac
    10801 cgacgttcgc gatcgaggtg gcgcggggcc tcccgaccgt cgccgtggtc gcctgcgccg
    10861 acgcggccgt cgccgaaagg ctccagaaag ggctggcgtc tccgacgttc cgcctctatc
    10921 gctcgtcgga catcgtcgga gtcgagatcg cgggcgcgac gaagaacgtc gtcgcgatcg
    10981 cggccggtat cgtcgacggc ctgaagctcg ggcacaacac ccgggcggcc ctcctgacac
    11041 gggcgctcgc ggagattcgg aggctgggga cgcggctcgg cggccagccg gagaccttct
    11101 ccggtctcgc cggggtcggc gacctgatcc tcacggcgac gggcgacctc tctcggaacc
    11161 ggcgcgtcgg gctcgccctc gcgtccggcc tgagcgtcga cgaggctctc gcgtcgctcg
    11221 gcggccaggt cgccgagggg gtcccgacgg ccggcgccat cgtggagctg gcccggaccg
    11281 tcggagtcga gatgccgatc tccgagaccg tcaggtcgat cctcgagggg acgacggctc
    11341 cccgggaagc ggtctcccgc ctcatgacgc gctcgctcaa ggaggaaggg gcctgacgta
    11401 cgcccgggtc gaccgtcccg gcgaggagcc gcccgcggag cgcacgttcc tcgacgtggc
    11461 cgtcctcgac atgaaccacg gcttcccgaa cctggggcac gcctcggtcg tcgggcgtct
    11521 cgagcggatc ggcgcggagg agcgcgagcg gctcggcgga ggcgctcccg aggtccgcgt
    11581 ggtctcgtac gacgtccggc gcggcctcgc ggtcccgtcg agcgccgacc gcttcccgct
    11641 cgtcgtcggg acgggcggac cgggcgccct cgacccccgc gagaacgacg gcatctcgcc
    11701 gctgtcccag gggatcgacg aggacccggc gtgggaggcc ccgctctggc gcttcttcga
    11761 cgcccttctc ggccggagcg acggcgcgct cttcggcatc tgccacacct tcggcctgct
    11821 ggcccgatgg tccggtttcg gcgaagccgt ggcgcgcggg ccggagaagg ggaagagcgc
    11881 gggggccgta acgaaccgcc tcacgcccga ggccgaggcg cacctctggt tcggcgggta
    11941 ctaccggacg agctccggcg ggagggtccg cgtcctcgac agccgcctct tcgacatcct
    12001 tccgtcgggg cgcgggccgg gcacgatcct ggcctgggag tcgaacgacg ccggcgagcg
    12061 cggagacgcg gcgaccatgg tcgagctgga ccgctacgcg gacggggccc cgcgcgtctg
    12121 ggcggtgaac caccaccccg agatcggcgg agccgcggag cagcgggaac gcctgctgcg
    12181 cctcgcctcc gagcgggagg tcgggccggg ttggctcgag gagaggctgc gggcgctgga
    12241 cgccttcgac gtctcccccg aggtggaaaa ggagcttcgc gcgacgcggg cgttcacgtt
    12301 cgaggagccc gtccgcgccg tcctcgcccg ggcgctcgcg gagcgctcgg ggacggcctg
    12361 atcgggctca gacggtgccg tggaggtcgc agtgatcgcc gtgcaggtga tggagagcgc
    12421 cgtcgacgag atagtcgaag tggtcgccgt gagggacgcg cggatgaggg catcccgggt
    12481 gatgtcgggc ggggcactcg accggagcgc acgccgccgg gtggccgtgg tcgacggcga
    12541 ggacgtgctc ggccacgccc gactcgccgg gagaatgcag gtgcccgtcg tggagataat
    12601 cggcgtggtc gccatgccga atcgcccgat gaccgcagcc cggcccgtgc tcgtggggat
    12661 gcccgtccct gtgctccacg gcgtgtcgcc ctcctcgggc gggagcatcg ggccgcggac
    12721 ggcccgaggt caagctgcgg gagcgcgacg atgacccggg cccgcgtgcg cctggcgatc
    12781 ggcctttcgc ttgcggcagc gggctgcgcg ccttcgtcgt cgcgcccggc agccggccct
    12841 caggtcgccg tcgcgacgat cgccccgctc gccgacctcg tccggcgggt cgccgggccc
    12901 gagtggaccg tgcggaccgt gatccgtccg ggaacctcgc cgcacgtctt cgagccggag
    12961 ccgtccgatc tccggaagct cctcggggcc cgggcggtct tcgtggcggg ggcgggctac
    13021 gacgactgga tgcggaaggt ccttgcggcg tgcgcctcgc gcgcgcccgt ccacgacgcg
    13081 gcggcctcta tcgggatcgg acctgggcgc gcgtggtcgg cgagcgccgg tcgcgatctt
    13141 cccgcgcagg gtcccgaggc ccacggcgag gacgatctcg tccacgcggc cggacctcac
    13201 gaccgggatg gtcacgcggc gcacgaccac gaagccgccg gccacgaggg gcacggacac
    13261 gaggacgacc cgggcggtgc ggcaggagct cacgacggcc acgcccacgg catcctcggc
    13321 gaggacccgc actggtggct ctctcccgag ctcgccgccc gaagcctcgg cggcatcgcc
    13381 gaggcgttcg cggcggtcga cccgatccac gccgagtcgt atcgggcccg ggcccgggag
    13441 gccgcggcgg agctcgtcga gctcgacggc gagatctcgg cgaagctcgc gccggtccgc
    13501 gggaagcggt tcgtctcggc gcaccgggcc tgggtctact tcgccgaccg ctacggcctc
    13561 gtcgaggcgg cctcgatcga gcccgtgccg ggtcgggagc cctccccgcg cgagctgaag
    13621 gcgctcgtga cggaaggccg gcagggaggg ctgggccgac tgttcaccga gccgcagttc
    13681 ccgccggcgg cggcgcgggt cgtcgcgaac gaggcgggcc tcgacctgac gctcgtcgac
    13741 ccgatcgggg gagtccccgg ccgatcgacg tatcctgagc tgatgcgctt caacgcggac
    13801 gccttccttc gggggctctc cggcgcggcg gagagacgcc cttgagcgtc gccgcctgct
    13861 tcgagaacgt gacggtccgc ttcgggggcc ggccggcggt cgagtccacc tcgttcacga
    13921 ttccggccgg gatgcgcacg gcgatggtcg gaccgaacgg cggcggcaag acgacgctcg
    13981 cgcgggtcct cctcgggatc ctccggccgg acgccggccg cgtcgcattc ctggacgagc
    14041 aggggcggga gctgccgcgg ccgaggatcg gctacctccc gcagcgctcc acgctctcgc
    14101 cgcaggcccc cgtgaccggc ctcgacctcg tcggcctctc cctcgcgccc gggccgggct
    14161 tcgggatgtc gcccgcggtc gaggcggagg cgcgccgggc gctcgggaga gccggcctcg
    14221 ccgacgacct cgcgacgcgc cccgtcggcc gcctctcggg cggccagcgg cagcgtgtcc
    14281 tgctggcccg cgcgatcgcc gggacgccgg cgatcctcct cctggacgag cccgacacgg
    14341 cgctcgacgc cgaggggatg aagacgctcc gccggctcgt cgcggaagag gtcgccgcgg
    14401 ggcggacgat catctacgtc acgcacgaca gcgacgcgat gggaggagcc gacgccgtct
    14461 tccggatcga cgggcggctc acggaggaga cgcgcgcgtg acggagttcc tcgccttccc
    14521 gttcttccag cgggcgctcc tcgccggctt cgtcgtggcg gtcgtctgcg gggcgctctc
    14581 gttcttcgtc gtcctccgga ggctcgcgtt cgtcggctcc ggcgtctccc acgcggcctt
    14641 cggcggcgtc gccctcgccg cgctcgtcgg cgcgccgccc gggctcgggg cgctcctggc
    14701 ggccctcgcg gtcgccttcg cgacggccaa agcctccggc gacggcgacc tgaccgaaga
    14761 caccgcggtg ggcgtcttca ccgtcgcggg gatggcggtc ggcgtcgtcg cgatcagctt
    14821 cctcgagacg aacgtcgacc tcttcggcct catgttcggg aacatcctga cggtcgaccc
    14881 g
//
LOCUS       DAOTEZ010000055        14797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2788_length_14797_cov_5.670126, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000055 DAOTEZ010000000
VERSION     DAOTEZ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14797
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2788_length_14797_cov_5.670126"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="P5164_04495"
     CDS             <1..1077
                     /locus_tag="P5164_04495"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43187.1"
                     /translation="LRLVPAKLAANLFVVPFKRLGRNLSLAMRDPKDLPAIDEVSFLT
                     GLAITPFVALDIRIQLALQKHYGIAIDARYPDLAARLEAGGGMLPVPPPRTPRPLVRS
                     SSTGARPVAAETPPAPGPVPPAAVQPTPLPASIPPPSVPSPAAPEPVAAPAAKPAAPA
                     EIEMEAHEEERLRISGPIVLSPEPTDAVGGEEVAPLVEEAAPATLVECLSRAESRDEI
                     AAAVLREAGRLLRRAALFIAQAERVIGWAAVPEPPDGLRSFSIGFAEPSLFASLRNTD
                     GFYVGPCPDLAGNRKILEALGADFPAHVAAVPVTLKGKSVLFLLGEVRPGETPPKAME
                     LKRLGALTAIALEILLLRNKLRSL"
     gene            1105..1437
                     /locus_tag="P5164_04500"
     CDS             1105..1437
                     /locus_tag="P5164_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRY43188.1"
                     /translation="MIKNDLVNRVTEETGVPRVQASLAVETIIEAMRRSLAGGGRIEL
                     RGFGVFLVKRRKRGIGRNPKRPDEQVEIPPGFTIRFKPGKELRDLMVVEPEAPGAEPA
                     EASTPEPA"
     gene            complement(2430..2885)
                     /gene="dtd"
                     /locus_tag="P5164_04505"
     CDS             complement(2430..2885)
                     /gene="dtd"
                     /locus_tag="P5164_04505"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRY43189.1"
                     /translation="MILLLQRVSRAAVRVGGVSVGEIGPGLLVFGCVEPGDGPDAMDE
                     AARKTVELRVFEDGQGKMNLDVTQSGGAVLAVSQFTLGADLSRGRRPGFEGAARPEEA
                     RPLFDRYLAALRASGVPVATGSFGAAMEVELVNDGPATFHWRTKRRPES"
     gene            2968..3390
                     /gene="ssb"
                     /locus_tag="P5164_04510"
     CDS             2968..3390
                     /gene="ssb"
                     /locus_tag="P5164_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010547018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRY43190.1"
                     /translation="MAGSVNKVILVGHLGADPEMKALPSGMQMAKLRVATSETWTDKT
                     SGQRQEKTEWHNVIVYDKLAGICERYLSKGQLVFIEGSIQTRSWDDKESGQKRYMTEI
                     KARDMRMLGGRPDGARGGAAPAAPEPPMDGDPDDSVPF"
     gene            complement(3394..4458)
                     /locus_tag="P5164_04515"
     CDS             complement(3394..4458)
                     /locus_tag="P5164_04515"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRY43191.1"
                     /translation="MNGARLVVGVAGTALSVAERAFLSESRPAGVLLFPENLASAEQA
                     VDLVAEVRAACSPSPLLFVDQEGGPVDRLGPLLGFSFPSPAALSAMGVHRVHEGAYLM
                     GRAARLLGFDVDLAPVLDLEQPGTGAVVLAGRTFGFHAEDVVVAGSVFLHGLARAGVA
                     SCVKHFPGLGRGPVDSHGALPVVDAHDVDLMVTDVAPFTKLARSSDGVLVGHAAYPGF
                     TGDETPASLSPRIHAILRERIGWDGVVLSDDLGMGALAAIPFAERVVRAARAGCDLLI
                     VSQGLDAAVAAAGALAAESIDREPAALERIAALRRRCDGPPRTPFGPEAWDALRAEGE
                     SWLSDLTKPRVRPDDSAFGL"
     gene            complement(4455..7073)
                     /gene="mutS"
                     /locus_tag="P5164_04520"
     CDS             complement(4455..7073)
                     /gene="mutS"
                     /locus_tag="P5164_04520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRY43192.1"
                     /translation="MRTTPMLEQYWALKREVPDALLLYRLGDFYEMFFEDAEAAAPLL
                     GLVLTARHRDTDVEAPMCGVPHHALESYAARLIAAGRKVAIAEQVEAPQKGRTLVARK
                     IVRVITPGTLLDPERLDARQANDLAAIALGGDEAAFAFLDLSTGEFAAVRLDGTPPAE
                     LLARRPAKELVAFPSERDAVARMLEAFGPEAPVLSVLPSESPRGRSAAELLEGHFRTS
                     TLSAFGLPASGPLVEAAAAALSYARSTQRSDCAHVSGLRTESATGGLVVDAVTQGHLE
                     LFRSLRDGGRNGTLLSVVDRTGTSFGARALRRVLEQPLGSIEEIEERLDAVDELVAGP
                     ARLEAVAAALARLPDLPRLLSRVAVGSATPRDLASLGTGLEQAAAVGPLLEGAESALL
                     RPAGHAFPSGVPDALSVRLAATLVETPPASAKEGGIVRDGADAGVDELRTLRRDAATL
                     LGALEAEERARRGIPTLRVKFNQVFGHVFEVPGSARSKIPEEALKRQTLASVERYATA
                     ELVALDEKLRSADEKLAEREGEIFRELCAAATTESAAVTSLSGRLALLDVLVSFARVA
                     RAERWCRPRLVAEPVLSIVDGRHPVVEALRRREPFVPNDTDLGGDARVVILTGPNMGG
                     KSTYLRQNALAVLLAHAGAFVPAAAATVGLCDRIFTRVGASDSLARGESTFLTEMAET
                     AHILRHATRRSLVVLDEIGRGTATFDGLSLAWAIAEHLHDAPGAPARVLFATHYHELT
                     ELALVREGVKNRTMSVKEWKGEVVFLRKVVDGTADRSYGVQVAKLAGIPAAVTERAKE
                     ILRNLERQQLDVGGRPRLAEHESAPAPSEAQLDLFRGQEEVVVDALRRVDVESLTPLA
                     ALNLLASLRARLEGLE"
     gene            7159..7755
                     /gene="tsaB"
                     /locus_tag="P5164_04525"
     CDS             7159..7755
                     /gene="tsaB"
                     /locus_tag="P5164_04525"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRY43193.1"
                     /translation="MRLLAIDTATPRPSLALVVDGSEPWTVELPQKGAEALAPAVSSL
                     LAAAGMPPRGLDRIAVVSGPGSFTGLRSSLAFARGLARAAGAILVPLPTFDVVSRACP
                     EPPDADLLIDALRGEVHRRRRRQGRLEPAEERVARSRAVAEADRDGVAALEIAALAPL
                     LAPAAASLAATAAPDGAAGLHYGRPPAAEERFGPGGAG"
     gene            7752..8228
                     /gene="rimI"
                     /locus_tag="P5164_04530"
     CDS             7752..8228
                     /gene="rimI"
                     /locus_tag="P5164_04530"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HRY43194.1"
                     /translation="MSALPAELTVRDVRNVDLDAVAELERLSFPVPWKREFFASEVGA
                     PHRFNRVVRTPDGRLAGYVFCAFAGGEVHVNKIAVDPSYRRRGIAHLLMQEVLDLSAR
                     VRAEEIYLEVRPSNDPARNFYSSLGFRDVGRRPQYYADGEDALVMSLFLPAPSPSP"
     gene            8294..8533
                     /locus_tag="P5164_04535"
     CDS             8294..8533
                     /locus_tag="P5164_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HRY43195.1"
                     /translation="MPTLTEELKRELAQTHDEFKNLVRTHHDFEKRLAELASKTFLSS
                     DEEGEEKRLKKQKLIVKDRMEEIAREHRARVGAPH"
     gene            8576..9340
                     /gene="pssA"
                     /locus_tag="P5164_04540"
     CDS             8576..9340
                     /gene="pssA"
                     /locus_tag="P5164_04540"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HRY43196.1"
                     /translation="MNGTSSEVQRRGARRGVYVIPALFTVGNVFCGYLSLMAAVRGRY
                     DLAAGLIFLAGFLDSLDGRVARMTGSTSAFGEQLDSLSDVLSFGLAPAFLVFHWGLSA
                     FGRLGLFASFLYLVCGACRLARFNVQIHVVDKRWFVGLPIPAAAGALCGLIWVIPEPL
                     PDPRIATVFIGVTLVLGFLMVSTFRYRSFKDVDLRSRRSMRLVPLLGLVIAAVAAWRP
                     DVCLASLAVVYALSAPTARLFSALFPRRAPVEDGPA"
     gene            9337..10326
                     /locus_tag="P5164_04545"
     CDS             9337..10326
                     /locus_tag="P5164_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43197.1"
                     /translation="MIGLSVAVVDPGTLAGRDVKAVLRERSFPSSRLQLFHTGDSTSG
                     LLTEDDGEAALASPLEPDGLAGCQIAFLCGPSDATARFLSRRPPDGCLAIDVSGQRSS
                     GTCVDAVRAVLPTPLPEGDLLVLRDPAATVLADAIAAAEAAGAVAGVTAAIDRPASDL
                     GKDALDELFQQAIAIASFRSVPRERLGAQSAFNVFSPADTDAFDGRVAEDVRALLRRD
                     LPVTVLSARAGVFHGTTIRLEIRFVGPAPSAGELRSALLATGYGFSEADVDGPAGIVD
                     AAGRDETLLLGAWSAGSTGRLFLASDALRRPGALLAVRVAEAAIADRGLLPDA"
     gene            complement(10345..11889)
                     /locus_tag="P5164_04550"
     CDS             complement(10345..11889)
                     /locus_tag="P5164_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43198.1"
                     /translation="MPLSKLHARRAAVAVLLLGALSGAACRKGPLAGASGPAAAEARA
                     DALFLAEAPVVVPEEIGPELDAMGVRRLFVSAASLAAGGQIRPFPPPPSRVPRPVVLV
                     LMGEPGAEGGLRGGSPEALGEAWAAGTTRLVAEAKDWAEVLGVHVHLVPTAGDAAALA
                     TALGVLKSRLPGLTVSATLRAGAPEGTWKPLAGAVDEALVLTFGRRPETADAFVSEMT
                     EGEAKTFPIPFRLLAIPGSYGVAGSGARARRLPDGEMDKLSEDRNLDFDFASVLSSDP
                     GNIYSFKPRTGFEKARNLLSDDGGRARFDVLPFADLVRLVSASARWSAPRLAGRVFLV
                     DGVPRDGRLSGYPAIRALLTGKPWEPSLRVEAIEEKTRGGEVWLRVSNPGPTPSELSR
                     FGNQITIRLEGGVFTGLGAGDFDRFEIFASARDLTQPAPFGRATACRLFENFFAPEES
                     NELGPIRIAGKSPRMFVTYQLTLPDGRTIDGAEAEVAIPAPPAPRAATKAPARRQPPL
                     PRLKKR"
     gene            complement(11905..>14797)
                     /locus_tag="P5164_04555"
     CDS             complement(11905..>14797)
                     /locus_tag="P5164_04555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY43199.1"
                     /translation="VPSLLLGLGLFTGPLEAPLTSAGFVAVATSLVGLGALLAGQAFR
                     PGRGTAAAGAIGLVLVVWCAFASPSTVSLALLLAGASLLAAGLSGPASRFADADLLSK
                     AVAVALLVGAGALVTGAGAALGRLARAESRLEAAASRSMLERERLEEEGPAGWEERIS
                     SPELLPWTPGGSRTAMSDLARALWLRGRNDEFPERGDLLTVRDEQGRVLSSFGTMRPG
                     DEGRGTAIGLALPVPGTLGVLSRIPWPDADEEDPLLVGELGNPVHAERPVERIDFDGA
                     GRPLGRGRLERSELAGPLLAEVRRGGPGWMTEESQGRRWRMSVRGTGAGFVAWGVEVP
                     SPVFTLVSAVAAAETALPLALVVFLRRELAALARARLARTGGRRGTRLFGTFRGRLAL
                     AFLVSGAVPLALGAALARTALDRSTARTTERHALELLREGRRILEDRVAGSPSPSDLN
                     QAASVVGVDLLLYRDGRLAAASRAVPVAAGLAPERLPTALAAALADGASSAARTRSPL
                     RPGGRRVAQAAVTLSREERTTLAVVLGEDPAGRTAVEGLFLLAAAVALGALAFGGRGA
                     LALSRPVEELVAASERIGAGEAPGPIRPPEMEDLARLVEAFGTMSERVRERTELLERE
                     REAAVSVLASLTPAALLFREETGKILYANPAADALLPTGAILGERLPQPRFAPVLAAL
                     DGPRPFESRVTLHGEGRETVLRVVVADMTADAAGARAVLLLEDLTDFTRAERLEAWVE
                     AARAIAHDIKNPLTPIRLTAERLLRGAGRGAPPDGARVAEAAETILRQVDLLTERTGR
                     LSRFASPAAPERTRLSGESLAGLLREVAADFGAHPAVRVDYAVEGELPEVLVDRALLR
                     DAVANFVLNAIEALEPRGGTVWVEARPAAGPRGGAGLEIACEDDGPGVTETDLVRLFD
                     PTFSTKSRGSGMGLAAARRAIEEHGGRLFAERSARGGLRIGFVLPASGR"
BASE COUNT         1974 a         5585 c         5263 g         1975 t
ORIGIN      
        1 ctccgtctcg ttcccgcgaa gctcgccgcg aatctcttcg tcgtcccgtt caagcggctc
       61 ggaaggaacc tctcactcgc catgcgcgac ccgaaggacc tcccggccat cgacgaggtt
      121 tccttcctga cggggctcgc gatcacaccc ttcgtcgccc tcgacatccg gatccagctc
      181 gcgctccaga agcactacgg gatcgcgatc gacgcccggt accccgacct cgccgcccgt
      241 ctcgaggcgg gcggcggaat gcttccggtg ccgccgccac gaacgccgcg cccgctcgtg
      301 cgttcgtcgt cgaccggagc gcgccccgtc gcggcggaga cgccgcccgc gcccggcccg
      361 gttcctccgg cggcggtcca gccgacccct cttcccgcat cgatcccgcc gccgagcgtg
      421 ccgagcccgg cggcgcccga gcccgtcgcg gccccggcgg caaagcccgc cgccccggcc
      481 gagatcgaga tggaggcgca cgaggaggag cggcttcgga tcagcggccc gatcgttctc
      541 tctcccgagc cgaccgacgc cgtcggaggg gaagaagtgg cccctctcgt cgaggaagcg
      601 gcgccggcca cgctcgtgga gtgcctctcc cgggccgagt cccgggacga gatcgccgcc
      661 gccgtcctgc gcgaggcggg tcgactcctc cgccgggccg cgctcttcat cgcgcaggcc
      721 gaaagggtga tcggctgggc ggccgtaccc gaaccgcccg acgggctccg ctcgttctcc
      781 atcggcttcg ccgagccctc gcttttcgcc tctctccgga acacggacgg cttctacgtc
      841 ggcccctgtc ccgatctcgc cggcaaccgg aagatcctgg aggccctcgg cgcggacttc
      901 ccggcccacg tggccgccgt tcccgtgacg ctcaagggca agagcgtcct cttcctcctg
      961 ggcgaggtcc gccccggcga gacccctccg aaggcgatgg agctcaagcg gctcggggcg
     1021 ctgacggcga tcgcgctgga gatcctcctc ctgcggaaca agctccgtag cctctagcag
     1081 ggacttggct tacacttcgc gggaatgatc aagaacgacc tcgtcaaccg cgtgaccgag
     1141 gagaccggcg tccccagggt ccaggcgtcc ctggccgtcg agacgatcat cgaggcgatg
     1201 cgtcgttcgc tcgcgggagg gggacgcatc gagcttcgcg ggttcggggt cttcctcgtg
     1261 aagcgacgga agcgggggat cgggcgcaat cccaagcggc ccgacgagca ggtcgagatc
     1321 ccgcccggct tcacgatccg gttcaagccc ggcaaggagc tccgcgacct gatggtcgtg
     1381 gagccggagg cgccgggggc cgagccggcg gaagcctcca cgccggagcc ggcctgagcg
     1441 gcctcgtccc gccgcagctc ccgcactccg cgaccgagga accggccccc gtcgtcccgt
     1501 cgttcgaccc ggccttcggc gttcccgacc acgcgggagc cgctccgccc atccgccggg
     1561 agcgctggtg gctccacgtc ctcctcttcg tcctgacgtt cggaacgacg tcgttcgtcg
     1621 gcgtgcagct cgccgccggg tacgacccgc ggctcgccgt cgtcgatccc cgttcgggag
     1681 tcctcggcat cacgccggag ctcgtcctgg ccggcctcgg ctactccgtg ccccttcttc
     1741 tcatcctcct ggcccacgag ctggggcact acctcgcctg ccgccggtac ggtgtgaacg
     1801 cttccccccc gtacttcctc cccttcctcc cgctcgtccc cctgccgggg acgttcggcg
     1861 ccttcatccg ggtccgggag ccgatccgcg agaagaaggt cctgttcgac atggcggtcg
     1921 cgggtccgat cgccggtttc ctggtcgcgc tcccgatcgc cgcctggggg atctggcaca
     1981 cccgcctcaa cttcgaccct cccgccgacg ggacgatcta cttcggctac ccgctcgccg
     2041 tgaagctgct ccagcttctc ctgaccgggc acacctactc gagcgtccac gtcgtggaac
     2101 acccggcgtt cctcgccgcc tggtggggct tcgtggttac cgccatcaac ctgatccccg
     2161 cgggtcagct ggacggagga cacacgctct acgcggtctt cgggagacgg caccggctgc
     2221 tgcgctggcc cgtcctcctc gttctcgcgg gcctcggatt cctctatccg gggtggtggc
     2281 tctgggcggt catcgtcctc gtcctgaccg ggctccggca cccgcccgtc ctcgacgagg
     2341 acgccccgct cgaccctcgc cggaaggcga tcgcggtggg cgtcgccctc ctcctcgtcc
     2401 tcagcttcgt cccgatcccg atccgggagt cagctttccg gccgacgttt cgtgcgccag
     2461 tggaacgtgg cgggaccgtc gttcacgagc tcgacctcca tcgcggcgcc gaagctcccg
     2521 gtcgcgaccg gcacgccgga ggcgcgaagg gccgcgaggt agcggtcgaa gagggggcgc
     2581 gcctcctccg gccgggccgc tccctcgaag ccgggccgcc gcccgcgtga gaggtccgct
     2641 ccgagcgtga actgggagac ggcgaggacg gcgccgccgg actgcgtcac gtcgaggttc
     2701 atcttcccct ggccgtcctc gaagactcgc agctcgacgg tcttccgcgc ggcctcgtcc
     2761 atcgcgtcgg gcccgtcgcc cggctcgacg cacccgaaga cgaggaggcc aggcccgatc
     2821 tcgccgaccg ataccccgcc gacgcggacg gcggctcggg aaacgcgctg aaggaggagg
     2881 atcatcgcgc agattctcgc agggggatcg cgcagcccct ctgatatcct cgcgcgccat
     2941 tcgatctcga ttgcaggagg aacgcccatg gcgggttccg tgaacaaggt catcctcgtc
     3001 ggccacctcg gggccgaccc cgagatgaag gcgctcccct cggggatgca gatggcgaag
     3061 ctgcgcgtcg ccacgtcgga gacgtggacg gacaagacgt cgggccagcg gcaggagaag
     3121 accgagtggc acaacgtcat cgtctacgac aagctcgccg gcatctgcga gcgctacctc
     3181 tcgaagggtc agctcgtctt catcgagggc tcgatccaga cgcggagctg ggacgacaag
     3241 gaatcgggcc agaagcgcta catgaccgag atcaaggcgc gcgacatgcg gatgctcggc
     3301 gggcggcccg acggcgcccg gggcggcgcc gcccccgcgg ctccggagcc gccgatggac
     3361 ggcgacccgg acgacagcgt tccgttctga ccctcagagc ccgaaggcgg agtcgtccgg
     3421 gcgcacccgc ggcttcgtca ggtccgagag ccacgactcc ccttcggccc ggagcgcgtc
     3481 ccacgcctcc gggccgaatg gcgtccgggg agggccgtcg cagcggcgcc ggagcgccgc
     3541 gatcctctcg agcgcggccg gttcccggtc gatcgactcc gccgcgagcg ccccggccgc
     3601 cgcgacggcg gcgtcgagcc cctgggagac gatgaggagg tcgcagccgg cgcgagcggc
     3661 gcggacgacc cgctccgcga acgggatggc cgcgagcgcc cccatgccga ggtcgtccga
     3721 gaggacgacg ccgtcccacc cgatcctctc gcgcaggatc gcgtggatcc tcggcgagag
     3781 cgaagcaggc gtctcgtccc cggtgaagcc ggggtaggcc gcgtggccca cgaggacgcc
     3841 gtcggacgag cgggcgagct tcgtgaacgg ggcgacgtcc gtcaccatca ggtcgacgtc
     3901 gtgcgcgtcg acgacgggga gcgcgccgtg cgagtcgacc ggcccccgcc ccagccccgg
     3961 gaagtgcttc acgcacgagg ccacgccggc ccgcgcgagc ccgtgcagga agacgcttcc
     4021 ggcgacgacg acgtcctccg cgtggaagcc gaacgtccgc cccgcgagga cgacggctcc
     4081 cgtcccgggc tgctcgaggt cgagcaccgg cgcgaggtcg acgtcgaagc cgaggaggcg
     4141 cgccgcgcgc cccatcaggt aggccccctc gtggacccga tggacgccca tcgcggagag
     4201 ggcggccggc gacggaaagg agaaaccgag gagcggcccg agccggtcga ccggcccccc
     4261 ctcctgatcg acgaagagga gcggcgacgg gctgcaggcc gcccggacct ccgcgacgag
     4321 atcgacggcc tgctccgccg aggcgaggtt ctccgggaag aggaggaccc cggccgggcg
     4381 gctctccgaa aggaacgccc tctccgcgac ggacaacgcc gtcccggcga cgccgacgac
     4441 gagccgggcg ccgttcactc gagcccttcc agccgcgcgc ggagcgaggc gaggaggttg
     4501 agcgcggcga gcggcgtgag cgactcgacg tcgacccgcc ggagcgcgtc gacgacgacc
     4561 tcctcctgcc cgcggaagag gtcgagctgg gcctcggacg gggccggcgc gctctcgtgc
     4621 tcggcgagac ggggccggcc gccgacgtcg agctgctgcc tctcgaggtt ccggaggatc
     4681 tccttcgccc gctcggtgac ggccgccggg atccccgcga gcttcgcgac ctggacgccg
     4741 taggaccggt cggccgtgcc gtcgacgacc ttccggagga agacgacctc gcccttccac
     4801 tccttcaccg acatcgtccg gttcttcacg ccctcgcgga cgagagcgag ctcggtcagc
     4861 tcgtggtagt gcgtcgcgaa gagcacccgg gccggggctc cgggggcgtc gtgcaggtgc
     4921 tcggcgatcg cccaggcgag cgagaggccg tcgaacgtcg ccgtgccgcg gccgatctcg
     4981 tcgaggacga cgaggctccg gcgcgtcgcg tggcggagga tgtgcgccgt ctcggccatc
     5041 tcggtcagga acgtcgactc cccgcgcgcc agcgagtccg acgcgccgac gcgcgtgaag
     5101 atccggtcgc agagaccgac cgtcgccgcg gccgccggca cgaacgcgcc ggcgtgcgcc
     5161 aggagcacgg ccagggcgtt ctggcgaagg taggtcgact tgcctcccat gttcggcccg
     5221 gtcaggatga cgacgcgcgc gtcgccgccg aggtcggtgt cgttcgggac gaacggctcc
     5281 cgcctgcgga gagcctcgac gaccgggtgg cggccgtcca cgatcgacag gaccggctcg
     5341 gcgacgaggc gcgggcggca ccagcgttcc gcgcgcgcga ccctcgcgaa cgagacgagg
     5401 acgtcgagca gggcgagccg tccggagagg gacgtgaccg ccgccgactc cgtcgtcgcg
     5461 gcggcgcaga gctcccggaa gatctcccct tccctctccg ccagcttctc gtccgccgac
     5521 cgcagcttct cgtcgagggc gacgagctcg gccgtcgcgt agcgctcgac ggaggcgagc
     5581 gtctgcctct tcagcgcctc ctcggggatc ttcgaccgcg ccgagccggg gacctcgaag
     5641 acgtgcccga agacctggtt gaacttgacg cggagcgtcg ggatgcctcg ccgcgcccgt
     5701 tcctcggcct cgagggcgcc gaggagcgtc gccgcgtcgc gccggagcgt tcgcagctca
     5761 tcgacgccgg cgtccgcccc gtcgcggacg attcccccct ccttcgcgga ggcgggcggc
     5821 gtctcgacga gcgtcgccgc gagccggacc gagagcgcgt ccgggacgcc cgacgggaac
     5881 gcgtgccccg cgggccgcag cagagccgac tccgcgcctt ccagaagcgg acccacggcc
     5941 gccgcctgct cgagccccgt cccgagcgag gccagatcgc gcggcgtcgc gctccccacg
     6001 gcgacccgcg agaggagccg cggcaggtcc gggagccggg cgagcgcggc cgccacggcc
     6061 tccaggcggg ccggccctgc gacgagctcg tcgacggcgt cgaggcgctc ctcgatctcc
     6121 tcgatcgagc cgagcggctg ctcgaggacc cggcgaagcg cgcgggcgcc gaacgacgtg
     6181 ccggtccggt cgacgacgga gaggagcgtg ccgttccggc cgccgtcccg gagagagcgg
     6241 aagagctcga ggtggccctg cgtgacggcg tcgacgacga ggcctcccgt tgccgactcg
     6301 gtccggagac cggacacatg ggcgcagtcg gaccgctgcg tggagcgcgc gtaggagagg
     6361 gccgcggcgg ccgcctcgac gagcggtccg gaggcgggga gaccgaacgc cgagagggtc
     6421 gaggtccgga agtgcccttc caggagctcc gcggcgctcc gcccgcgcgg ggactcggac
     6481 ggaaggaccg agaggaccgg agcctccggc ccgaacgcct cgagcatccg cgccacggcg
     6541 tcccgctccg agggaaaagc caccagctcc tttgcgggcc gccgcgcgag gagctcggcc
     6601 ggcggggtcc cgtcgaggcg gacggcggcg aactcgcccg tcgagaggtc gaggaaggcg
     6661 aaggcggcct cgtcgccgcc cagcgcgatc gcggcgaggt cgttcgcctg gcgcgcgtcg
     6721 aggcgctcgg gatcgaggag cgtgccgggc gtgatgacgc ggacgatctt ccgggcgacg
     6781 agcgtccgcc ccttctgggg ggcctcgacc tgctcggcga tggcgacctt ccggcccgcc
     6841 gcgatgaggc gggccgcgta cgactcgagg gcgtggtgcg gcacgccgca catcggggcc
     6901 tcgacgtcgg tgtcgcgatg gcgcgcggtc aggacgaggc cgagcagcgg cgcagccgcc
     6961 tccgcgtcct cgaagaacat ctcgtagaag tcgccgaggc ggtagaggag gagcgcgtcg
     7021 ggcacctccc tcttcagcgc ccagtactgc tcgagcatcg gcgtcgtccg catcggaagg
     7081 ggggcgatgc tagcaggacg gggcgcgccg ggcgcgaggc cgtccggctc cgcgcctgct
     7141 acgatctcct ctacgccgat gaggctcctc gccatcgaca cggcgacccc gcgcccctcg
     7201 ctcgccctcg tcgtcgacgg ttcggagccg tggacggtcg agctgcctca gaagggggcc
     7261 gaggcgctcg cgcctgcggt ctcctccctc ctcgccgccg ccggcatgcc gccccggggc
     7321 ctcgaccgga tcgccgtcgt ctccggaccg ggttcgttca cggggctgcg ctcgtccctc
     7381 gcgttcgcgc ggggcctcgc ccgtgcggcc ggggcgatcc tcgttccgct cccgaccttc
     7441 gacgtcgttt cccgcgcctg cccggagccg ccggacgccg accttctgat cgacgccctc
     7501 cgcggcgagg tccacaggcg gcgacgtcgg caggggcgtc tcgagcccgc cgaggaacgg
     7561 gtggcgcggt cacgcgccgt cgcggaggcg gaccgagacg gggtcgccgc gctcgagatc
     7621 gccgcgctcg cacccctcct cgccccggcc gcggcctccc tggcggcgac ggcggcgccg
     7681 gacggggcgg ccggcctcca ctacgggcgc ccgccggcgg ccgaggagag gttcggaccc
     7741 ggaggcgccg gttgagcgcc ctccctgcgg agctcacggt ccgggacgtc cggaacgtcg
     7801 atctcgacgc cgtcgccgag ctcgagcggc tctcgtttcc cgtcccctgg aagcgtgagt
     7861 tcttcgcgtc cgaggtcggg gcgccccacc gcttcaaccg cgtcgtgagg accccggacg
     7921 gccgcctcgc gggatacgtc ttctgcgcgt tcgccggggg tgaggtccac gtcaacaaga
     7981 tcgccgtcga cccctcgtat cgccggcggg ggatcgccca tctcctgatg caggaggtcc
     8041 tcgacctgtc ggcccgggta agggcggagg agatctacct ggaggtgcgg ccttccaacg
     8101 acccggcgcg taacttctac tcctcgctgg gcttccggga cgtcggacgt cgcccgcagt
     8161 actacgccga cggcgaggac gcgctcgtga tgtcgctctt cctgccggcc ccgtcgcctt
     8221 cgccttgact ctcgcccttt gaaaaaggga gactcgcgcg ggctctttcg aaatccactt
     8281 cgaggaggtg tccatgccga ctctgaccga agagttgaag cgcgagctgg cccagaccca
     8341 cgacgagttc aagaacctcg tccgtaccca tcacgacttc gagaagaggc tcgccgagct
     8401 ggcttccaag acgttccttt cgtccgacga agagggcgag gagaagcgcc tgaagaagca
     8461 gaagctgatc gtgaaggacc ggatggagga gatcgctcgt gagcaccggg cccgcgtcgg
     8521 agccccccat tagggccggc tcgcgctcga ccggaacggt cccgtcgaca cacccatgaa
     8581 cggaacctcg agcgaggttc aacgccgcgg cgctcgccgc ggcgtttacg tcatcccggc
     8641 cctgttcacg gtcgggaacg tcttctgcgg ctacctgtcc ctcatggccg cggtccgagg
     8701 ccgctacgac ctcgccgcgg gactgatctt cctcgccggg ttcctcgact ccctcgacgg
     8761 aagggtcgcc cggatgaccg gctccacgtc ggcattcggg gagcagctcg actccctctc
     8821 ggatgtcctg tcgttcggcc tcgccccggc gttcctggtc ttccactggg ggctctccgc
     8881 tttcgggcgc ctcggtctct tcgcctcgtt cctctacctc gtctgcggcg catgccggct
     8941 cgcccgattc aacgtccaga tccacgtcgt cgacaagcgg tggttcgtcg gcctgccgat
     9001 cccggcggcc gcgggcgcgc tctgcggcct gatctgggtg atccccgagc cgcttcccga
     9061 cccgcgcatc gcaacggtct tcatcggcgt gaccctcgtc ctggggttcc tgatggtctc
     9121 cacattccgc taccgctcct tcaaggacgt cgacctccgc tcccgccggt cgatgcgcct
     9181 ggttccgctc ctcggcctcg tcatcgcggc cgtcgccgcc tggcgacccg acgtctgcct
     9241 cgcctcgctg gcggtcgtct acgccctctc cgcgccgacg gcacgactct tctccgcgct
     9301 ctttccccgg cgcgcaccgg tcgaggacgg cccggcgtga tcggcctctc ggtcgccgtc
     9361 gtcgatccgg ggaccctcgc ggggcgggac gtgaaggccg tcctgcgcga gcggagcttt
     9421 ccttcctccc gcctgcagct cttccacacg ggcgactcga cctccggcct cctgaccgag
     9481 gacgacggag aagccgccct cgcctcgccc ctcgagccgg acggcctcgc cggatgccag
     9541 atcgccttcc tctgcggccc gtccgacgcg accgcgcgat tcctctcccg ccgcccgccg
     9601 gacggctgcc tcgcgatcga cgtctcgggg cagaggagct ccggtacgtg cgtcgacgcc
     9661 gtccgtgccg tcctgccgac cccgcttccc gagggcgacc tgctcgtcct gcgcgacccg
     9721 gccgcgacgg tcctcgccga cgcgatcgcc gcggccgaag ccgcgggcgc cgtcgccggc
     9781 gtcacggcgg cgatcgaccg ccccgcgagc gatctcggca aggacgccct cgacgagctc
     9841 ttccagcagg cgatcgcgat cgcttcgttc cggagcgtcc cgagggagcg gctcggagcg
     9901 cagtcggcct tcaacgtctt ctcccccgcc gacaccgacg ccttcgacgg acgggtcgcc
     9961 gaggacgtcc gggccctcct ccgacgcgac ctccccgtga cggtcctctc cgcccgagcc
    10021 ggcgtctttc acgggacgac gatccgtctg gagatccggt tcgtggggcc ggcgccttcg
    10081 gccggcgagc tcaggtccgc tctcctggcg accggctacg gcttctcgga ggccgacgtg
    10141 gacggacccg ccggcatcgt ggatgccgcg ggccgggacg agacgctgct tctcggcgcc
    10201 tggtccgccg gctcgacggg ccggctcttc ctcgcgtccg acgccctccg gcggccgggc
    10261 gcgctcctgg ccgttcgcgt cgccgaggcc gcgatcgcgg accgcggcct ccttcccgac
    10321 gcctgacggc ttcggcctcc tcttctagcg cttcttcagc cgggggagcg ggggctgacg
    10381 cctcgccggc gctttcgtcg cggcgcgcgg agcgggcggc gccggtatcg cgacctcggc
    10441 ctccgccccg tctatggtcc ggccgtcggg cagcgtcagc tggtaggtga cgaacatccg
    10501 aggcgacttc cccgcgatcc ggatcgggcc gagctcgttc gactcctcgg gcgcgaagaa
    10561 gttctcgaag agccggcacg ccgtcgcgcg cccgaacggc gccggctggg tcaggtcccg
    10621 cgcggaagcg aagatctcga aacggtcgaa gtcccccgcg ccgaggcctg tgaagacgcc
    10681 cccctccagc cgaatcgtga tctggttgcc gaagcgcgag agctcgctcg gcgtcggccc
    10741 cgggttcgag acgcgcagcc agacctcgcc tccgcgcgtc ttctcctcga tcgcctcgac
    10801 ccgaaggctc ggctcccacg gcttcccggt caggagcgcg cggatcgcgg ggtacccgct
    10861 caggcgcccg tccctcggga cgccgtcgac gaggaagacc cgccccgcga gccgcggcgc
    10921 gctccacctc gcgctcgccg aaacgaggcg gacgaggtcg gcgaacggga ggacgtcgaa
    10981 tcgggcccgg cccccgtcgt cggagaggag gttccgcgcc ttctcgaagc cggtccgcgg
    11041 cttgaagctg tagatgttcc cgggatcgct cgagaggacc gacgcgaagt cgaagtcgag
    11101 gttgcggtcc tcggagagct tgtccatctc gccgtccggc agccgccggg cgcgcgcccc
    11161 ggagccggcg acgccgtagc tgcccgggat cgcgagcagg cgaaacggaa tcggaaacgt
    11221 ctttgcctcg ccctcggtca tctccgacac gaaggcgtcg gccgtctcgg gccgacggcc
    11281 gaacgtgagg acgagcgcct cgtcgacggc gcccgcgagc ggcttccagg tcccctcggg
    11341 cgcaccggcc cgcagcgtgg cggaaaccgt cagccccgga agccgggatt tcaggacgcc
    11401 gagggcggtc gcgagggccg cggcgtcgcc cgccgtcggg acgaggtgca cgtggacccc
    11461 gaggacctcc gcccagtcct tcgcttcggc gacgaggcgg gtcgtccccg cggcccaggc
    11521 ctcgccgagc gcctccggcg aacctccgcg aagccctccc tccgcgcccg gctcccccat
    11581 caggacgagg acgaccggtc tcggaacccg ggacgggggc ggcggaaagg ggcgaatctg
    11641 tccgcccgcg gcgagcgagg ccgccgagac gaagagtcgg cggacaccca tcgcgtcgag
    11701 ctcgggaccg atctcctcgg gcacgacgac gggagcctcc gcgaggaaga gcgcgtcggc
    11761 acgggcctcg gcggcggcgg gaccgctcgc gccggcaaga gggcccttgc ggcacgcggc
    11821 gcccgagagc gcaccgagca ggaggacggc gactgcggcc cggcgcgcgt gcagcttgga
    11881 gaggggcatg gcgaaaaaat gatatcagcg accggaggcc ggaaggacga agccgatcct
    11941 caggcctccg cgggccgagc gctccgcgaa gagccgcccc ccgtgctctt cgatcgcccg
    12001 gcgcgccgcc gccagcccca tcccggagcc cctcgacttc gtggagaagg tcgggtcgaa
    12061 gagccggacg aggtcggtct ccgtgacgcc gggtccgtcg tcctcgcagg cgatctcaag
    12121 gcccgctccg cctctcggcc cggcggcggg gcgggcctcc acccagaccg ttcctccccg
    12181 tggctcgagc gcctcgatcg cgttcagcac gaagttcgcg acggcgtccc gcagcagcgc
    12241 ccggtccacg aggacttccg gcagctcgcc ctcgaccgcg tagtccacgc ggacggccgg
    12301 atgcgcgccg aaatcggcgg ccacctcccg aagcagcccg gcgaggctct cgccggagag
    12361 ccgggtccgt tccggcgccg ccgggctcgc gaaccgggag agacgcccgg ttcgctccgt
    12421 caggaggtcg acctgccgga ggatcgtctc ggccgcctcc gcgacccgcg ccccgtcggg
    12481 aggagcccct ctccccgcgc cccggagaag gcgctcggcg gtcagtcgga tcggcgtgag
    12541 cgggttcttg atgtcgtggg cgatcgcgcg ggcggcctcg acccaggcct cgagacgttc
    12601 ggcgcgcgtg aagtcggtca ggtcctcgag gaggaggacc gcgcgagctc cggcggcgtc
    12661 cgccgtcatg tcggcgacga cgacccgcag gaccgtctcc cgcccctccc cgtggagcgt
    12721 gacccgggac tcgaagggcc tcgggccatc gagggccgcg aggacgggag cgaatcgcgg
    12781 ctgcggaagc ctttcgccga ggattgctcc cgtcggaagg agcgcgtcgg cggccgggtt
    12841 cgcgtagagg atcttcccgg tctcctcgcg gaagaggagg gcggccggag tgaggctggc
    12901 gaggacgctc accgcggcct cgcgctcccg ctcgaggagc tcggtccgct cccggacgcg
    12961 ctccgacatc gtcccgaacg cctcgacgag ccgggcgagg tcttccatct ccggaggccg
    13021 gatcggcccc ggggcctcgc cggcgccgat cctttccgag gccgccacga gctcctccac
    13081 gggtcgcgag agggcgagcg cgcctcggcc tccgaacgcc agcgcgccga gggcgacggc
    13141 ggcggcgagg aggaagagcc cttcgacggc cgtacgtccc gcggggtcct ctccgaggac
    13201 gacggcgagc gtcgtccgct cctcccggga gagggtcacg gcggcctggg caacccgccg
    13261 cccgccgggc cggagcggcg agcgcgtccg ggcggcggag ctggcgccgt ccgcgagcgc
    13321 cgcggcgagc gcggtcggga gacgctccgg cgcgaggccg gcggcgaccg gcacggcgcg
    13381 cgacgccgcc gccagccgcc cgtcgcggta gagaaggagg tcgactccga cgaccgacgc
    13441 cgcctggttg aggtcggacg gcgaggggct ccccgccacg cggtcttcca ggatgcgccg
    13501 cccctcccgc aggagctcaa gggcgtgccg ctcggtcgtt cgcgccgtgg agcgatcgag
    13561 ggccgtccgg gcgagcgctg cgccgagggc gagcggaacg gcgccggaga cgaggaatgc
    13621 gagggcgagg cggccgcgga acgttccgaa gagccgcgtc ccccgacggc cccccgtccg
    13681 cgcgaggcgg gcgcgcgcga gcgccgcgag ctcccgccga aggaagacga cgagggcgag
    13741 cgggagcgcg gtctcggcag cagcgacggc cgagacgagc gtgaagaccg gagacgggac
    13801 ctccacgccc cacgcgacga agccggcgcc cgtgccgcgg accgacatcc gccacctgcg
    13861 tccctgcgac tcctcggtca tccagccggg gccgcccctg cggacctcgg ccaagagcgg
    13921 gcctgccagc tccgatcgct ccagccgtcc gcgaccgagc gggcgtcccg cgccgtcgaa
    13981 gtcgattctc tcgaccggac gctccgcgtg gaccgggttc cccagctccc cgacgaggag
    14041 cgggtcctcc tcgtcggcgt cgggccacgg gattcgagag aggacgccca gagtcccggg
    14101 gacggggagc gcgaggccga tcgccgtccc tcgcccttcg tcccccggcc gcatcgtgcc
    14161 gaacgaggag aggacgcgtc cctgttcgtc gcgcacggtc aggaggtccc cccgttcggg
    14221 aaactcgtcg tttcggcccc ggagccagag agcccgcgcg aggtcgctca tcgcggtccg
    14281 gctcccgccg ggagtccagg ggaggagctc ggggctcgag atcctctcct cccagccggc
    14341 cggtccttcc tcctcgagcc gttctctttc gagcatcgac cggctcgccg ccgcctccag
    14401 gcgggactcg gcgcgcgcga gccgcccgag ggccgcgccg gctccggtca cgagcgcccc
    14461 tgcgccgacg aggagcgcga cggcgacggc cttcgagagg aggtccgcgt ccgcgaagcg
    14521 ggatgccggg ccggagagcc cggcggcgag caggctcgcg cccgcgagca ggagcgccag
    14581 cgagaccgtc gacggggacg cgaacgcgca ccagacgacg aggacgagcc cgatcgctcc
    14641 cgccgcggcc gtcccgcgcc ccggacggaa cgcctgtccc gcgaggagcg ccccgaggcc
    14701 gacgaggctc gtcgcgaccg ccacgaatcc ggcggaggtg agaggcgcct ccagcggtcc
    14761 ggtgaaaagg ccgagaccga ggagcagcga cggcacc
//
LOCUS       DAOTEZ010000056        14480 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2888_length_14480_cov_5.426620, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000056 DAOTEZ010000000
VERSION     DAOTEZ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14480
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2888_length_14480_cov_5.426620"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..487)
                     /gene="rnpB"
                     /locus_tag="P5164_04560"
     ncRNA           complement(86..487)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="P5164_04560"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(523..1125)
                     /locus_tag="P5164_04565"
     CDS             complement(523..1125)
                     /locus_tag="P5164_04565"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HRY43200.1"
                     /translation="MVEAIAHFGTGLGLAAGAGLNAYAVLLVYGGIARLFPEDFPGAI
                     ARLLSEPAALSVLLALYVLEFFADKIPGLDHVWDIVHTVVRPAAGALLAVAAVAPGAE
                     TSMTVLAGGGGGVIALAAHVVKGTVRLTSTALTAGVANFALSLAEDILAALQAIVSIF
                     LPLVALVLAVAVGGLFLLCVPKLAKGVNLFTSRRRPRAVP"
     gene            complement(1155..2654)
                     /locus_tag="P5164_04570"
     CDS             complement(1155..2654)
                     /locus_tag="P5164_04570"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HRY43201.1"
                     /translation="MDTLKPGTGSLSGGTLEKTELADVLRSAYASRRHLQVLVSYRGE
                     ERSFWFRRGRLVSASSNREAQHVGDLLRTFGLANESVLFAAFERALADPGRGLASALR
                     DAGAVAPYVADACVRALAEMVLYSSFQWSAGTFSVVTLGSAPDLPVAFDQSTASLVLE
                     GLRRRPPVAGVAPRVDPQSKLVLSPDLLLRYQCAVVTAEEAEALDAVDGTKRAADVCL
                     DLRILERLISVGLVSVVDRKRPPDEQAPAGVQSLNVEVAGTPPSPRLAELLEQQSKTV
                     WNAYRRLDWTTAYELLGVAEDAADPDLRRAVHERARLFHPDNALRSTLADAGEALEAL
                     FAKIREADRSFASPTSRQQYDRLRASGGMSAPVQYGEPTREVQKQVAKANYQRARTLI
                     EMEDFFPAYEMLRQAVEFDPERPEYWTLLAKVQARNPKWIRQATETLRRATENLPDSV
                     EVWLAYADALGAERNETGRVKALKEVLKLDPTNRKASKALAEIAATKPR"
     gene            complement(2667..4475)
                     /gene="uvrC"
                     /locus_tag="P5164_04575"
     CDS             complement(2667..4475)
                     /gene="uvrC"
                     /locus_tag="P5164_04575"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRY43202.1"
                     /translation="MGASPDLSSYPDQPGIYLYRDAKGKILYVGKARSIRKRLASYFA
                     AGPKHPKTEALLAEFETIDTVVANTESEALALENAFIKKHKPRYNILLRDDKTYPYIK
                     VTTGEEWPRAHVTRRVLKDGHSYFGPFLPARTCRGAMRMVQRMFQVRTCRIEIDGTLP
                     RPCLYHDMHACLGPCVAGLTTKAAYDDAVRDVVLFLSGRNEELSPRLAAKMDAAAGAE
                     QFEMAAAYRDCLRTVEELAQKQRVQSLAGEDVDVFGDFADGGNVAVSVLHVRGGAVLD
                     SRELFWEGLGEVEPEEFWVALLSQYYDATTFLPREVHLPLEVAEEALEPLEAWLSDRR
                     RTKVAIRTPKRGAAFDRVRIARDNARTSHVRRFRTTREAGEKATLALAKALDLDRRPS
                     RIECFDISHFQGSDTYASCVVFVDGTAAKDEYRAFRIERPVPDDFASIAEAVTRRYER
                     RRAEGEPLPDLLVIDGGKGQLSAALAALDRVGIELPAVGLAKREEELFVPGRSLPITL
                     PRRNAGLKLLQRARDEAHRFGLSHHRRARSRRTLQSPLLAIPGLGPASVKRLLTVFGN
                     GEAVLAASPEALAAAAGKAVAARVERFRASSGGDRR"
     gene            complement(4514..6160)
                     /locus_tag="P5164_04580"
     CDS             complement(4514..6160)
                     /locus_tag="P5164_04580"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRY43203.1"
                     /translation="MRRFSPPAGPIIDGLDVETGRAFVTRNPATGAPLAEVRRGGAEE
                     IDAAVAAARKAFLHWGARSPKERRRHLEALLEAVRRSHDDLARLVAAEQGKPVAEARA
                     LDLVPAADALRYLSRHAEELLAEKPVDYEQLLFAHKAASDRFEPLGVIAVITPWNFPF
                     AIPMVEVAACLAAGNTVVLKPASATPRTGLAIGELCRMAGRPPGVVNVVPATGSDVDR
                     LLEHPAIAKILFTGSVETGRHVMRRAAGNVTPFVLELGGKDAAVVASDADLERTASGL
                     VWGAFANAGQACGSIERVYADRKIADELVSRVVEKTRRLRQGDPLAEATDLGPMTTAD
                     QRDFVDGQVREAISQGARALTGGERPAAKGNWFPPTVLVDVHHGMRVMTEETFGPVLP
                     IAVVDSLDEAIALSNDSEFALTASGWTRSTRTARKLVEELRAGTVTINDHLFSFGEPT
                     ASWGGLGKSGIGRSHGVHGLRELVNVKHVSVDLHASPSAPWWYPYDGAFQQFMRKAFG
                     VLYARDARTRVADGLGLLASGRFFGYVKVSTIASKLGKAL"
     gene            complement(6157..7275)
                     /locus_tag="P5164_04585"
     CDS             complement(6157..7275)
                     /locus_tag="P5164_04585"
                     /inference="COORDINATES: protein motif:HMM:NF019605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY43204.1"
                     /translation="MARKKASANPAPSRVVLFTGFPGFIGARLIPRLLELEPDTLFHC
                     LVQEKFLDLARSSVAQLAEAHPGTAGRIELVVGDITAPGLGIGETEARVLHRKLTGCY
                     HLAAVYDLAVPREVGMRINVEGTRNVLAFLEECPHLHRFDYVSTAYVSGTAVGLFRET
                     DLDVGQSFKNYYEETKFLAEEAVRGSALPTATYRPAIVVGDSRTGETAKFDGPYFALN
                     AMRQLPSPGVFVKVGTGTNPVNLVPVDFVLEAIARLSTWEGSIGKTYHLTDPRPLSAF
                     EIEELFAKALGKQFVYVPVPAVVAKLLFSPKPIQDWFGMPAVTVEYFDHPARYDSSQA
                     AADLARFDVSCPPFTDYVQNLVAFYRRHVEKITRGAMI"
     gene            complement(7299..8078)
                     /locus_tag="P5164_04590"
     CDS             complement(7299..8078)
                     /locus_tag="P5164_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRY43205.1"
                     /translation="MSPDPIPYDAPVVERGFGFFETLLVVGRRAVLWEAHLRRLLATL
                     EALELPAPSGDAVESAARSALAGIAEGDEERSLRLSWIAIGHDVEEPASWRLDASVRP
                     IPPNTLARRAGSRALTAPERFRRDSSGFKSTSYFGAVAALRHARKAGCDEALFRDGDV
                     YVEGSSTALVAWNGGRLAGLGPGGLPSVTAEAFLEGAGERRGVGREELLAGSFLLGSL
                     TTAAPVLSLDGEECARPPAMLRAIEAFRQRLRTDAALQKSL"
     gene            complement(8078..9280)
                     /locus_tag="P5164_04595"
     CDS             complement(8078..9280)
                     /locus_tag="P5164_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HRY43206.1"
                     /translation="MPSPFVLLRGPGWKLPEPLELRDPFEVLDVKADRPEELPHLLER
                     LDAVARSGRPELFAAGFLTYEAGVALEGSTALFRPPATPLAWFGLFDLRNAVRRAGSA
                     DAPSIRPDFARGDGREGPFGPAGEMTLEEWSAAVSEIRSGIERGDVYQANLTRRVVRA
                     LPVSPRRLADLLYGDNPVPYAILVATGAAAVVSNSPELFLELDPASRRVASAPIKGTV
                     ARTAGGSEAAARALLLSSEKDAAEHLMIVDLVRNDLGRVCRPGSVHVPALRRVKSYRH
                     LHHLESTVAGSLAPGAGLSDLLLATLPAGSITGAPKRSALGFIRRLEPAPRGPYTGAA
                     GFVRGDGTAVLNVAIRTAIVSGGAVAYHAGGGIVWDSDPRHEWEESETKSREFFRALA
                     AAAGESEV"
     gene            9301..9720
                     /locus_tag="P5164_04600"
     CDS             9301..9720
                     /locus_tag="P5164_04600"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRY43207.1"
                     /translation="MSEERRVETEVKVRYAETDAQGVVYHGSYVVWFEVGRTEYCEAA
                     GYPYPRMEAEGVQIVVVDLSARFRRPARYGDVVRVATRLAAIRSRGCTFAYRVFLPDG
                     GLAVEGETVHVFVDAATGRTIAAPEPIRSALAAFAAG"
     gene            complement(9725..10216)
                     /gene="coaD"
                     /locus_tag="P5164_04605"
     CDS             complement(9725..10216)
                     /gene="coaD"
                     /locus_tag="P5164_04605"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRY43208.1"
                     /translation="MRIAVYPGSFDPMTNGHVDLVRRGCHIFDRVLVAVLCNTEKAPL
                     FTVEERVQMTRDVFRREPKVKVKAFSGLLVEFLRAEKTNIVLRGLRVVSDFEYEFQMA
                     LMNRRLDPEVETVFLTPKEELSYLSSRLVKEVHRLGGNVSELVPSLVYRALQKKLPNG
                     KES"
     gene            complement(10416..11255)
                     /locus_tag="P5164_04610"
     CDS             complement(10416..11255)
                     /locus_tag="P5164_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY43209.1"
                     /translation="MTRGRVQRRLLVLALFPALALLAPLLARALPADAPQAIRALTPV
                     PFDPDATDLATRLRPPDGTHLLGTDDLGRDVLSRLLHGARVSVLVGLAAAALAFAAGT
                     LLGAAAGTLGPGVDRAVLVVTGVVQAFPALVLVAAGAAMAPPSRWTAALLIALTAWPE
                     AARLVRAEALRLSASPYVEAARAAGASPARVVLRHVLPGAIAPAIASLPYVVGGAVLL
                     EASLSFLGLGVPPPTASWGRALADARESLTSAWWCVLPPAAALFLLVLSARKAGEALS
                     ESR"
     gene            complement(11252..12199)
                     /locus_tag="P5164_04615"
     CDS             complement(11252..12199)
                     /locus_tag="P5164_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY43210.1"
                     /translation="MTRFLARRALGAVGTLVGVLVAVFLLLSAAPGDPARLAAGGMPG
                     RRPASAEALEAFRAVHGLDRPVVARLAAWLGSAVRLDLGRSFRDGRKVTERIGETLPA
                     TLAVNGTALLLAALGGVPLGVAAARRPGGRADRLAGLLLDALFSLPSFALGLLLLFLF
                     AVRLRWTPVFADASSPLASLALPSLTLALVALAPIARVVRESVRAALASPPAIAGRAR
                     GESPREETLRALRRSVPAFAGLLAALVPQLVAGSVLVERVFAVPGTGQLLADSVFARD
                     LPTVLGLTLASGAAVVLATLLADALAALADPRLVDAEDS"
     gene            complement(12237..13799)
                     /locus_tag="P5164_04620"
     CDS             complement(12237..13799)
                     /locus_tag="P5164_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY43211.1"
                     /translation="MKRLLVLALFLSGCGGPVGKDQSLVLHRRLEGEPQTLNALTATS
                     DPENVVIALLQRNLLDYDEHLDLVPGLAESVEPDESRLVYTVRLRPGARWEDGTPVSA
                     EDVKVTLEALLDPKTPALYRRSLFAELEKVDVLDPRTARATFRKAYAAQRDAFNMPLL
                     PAHVYRGTDVATNPANRRPLATGPYRLASWEGPTIRLVRNTQYFGTAPAYEQVVLRVV
                     PESASAFQGLLTGALDETRLNATQRPLAAKAGAIRETVYDEIAYTYIAWNNRLPQFSD
                     ARVRRALTMLLDREAIARELYGGLAKPANGPLPPGLWPHDATLRPIPHDPAAAAALLD
                     EAGWKKGKDGLRAKGKVRLEFSLVFGGASERQRQVMELAQRAFGEAGIEAKLSPMEWG
                     AFVEKVDTGDYEACSLSLNLDPNPDLRPNWHSSQVPPSGMNHAFYRNPRADALMDEIA
                     GTFDRERARVLYAELQRVIAEDQPFTFLHTVSVAWGVRDRIENVTSSPIGLWLFWPGA
                     ASWQPARGSKPI"
     gene            complement(13796..>14480)
                     /locus_tag="P5164_04625"
     CDS             complement(13796..>14480)
                     /locus_tag="P5164_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43212.1"
                     /translation="GAVFARSGAASGPAVLSVTNPDENGAFAGLEPEKPAETPGKKLF
                     RKGFRTVTWKGTDPNGDALRYDLEVRREGSVTWFPIRRDLEDPFFSFDTAPLPDGRYR
                     FRVTASDRAANLDGDALETSEESDVALVDGTPPSIVRTGARLEGGRVVLRVKAVDALS
                     PITRAEGTVSADRWRPLTPADGTLDGREEELTLTVPKPDGPAFLAIRVVDAAGNGASI
                     SFEYPEELR"
BASE COUNT         2054 a         5078 c         5408 g         1940 t
ORIGIN      
        1 aagtgacccg ggccggactg gctccggggg ggcttgccat ccgggcgccc ccccggcacc
       61 cccccacctt caatctgacg taagtgaccc gggccggact gtaagccgga ttctgtggcc
      121 ggtcggctcg cgccttccgg tcggcgatca ttcctctggg cccgcgcctc ccggcggggc
      181 tcgagcggcc tacccggaaa cgtcgccttt cgacgcgggg gcgagccccc cttccgttcc
      241 cctattcggc cttgctccgc gtggggtttg gcctgccgac cccgttgccg gggttcgcgg
      301 tgcgctctta ccgcaccttt tcacccttac cgcggcccct cgcgggaacc gaggcggtct
      361 gttttctgtg ccactttcct tcgggttgcc ccgaccggga tttccccggc acgctgctcg
      421 ctggagtccg gactttcctc ccctcgttcg gcggtgccgg gcgagcggcg atcgcccgtc
      481 cggcccgggt cggcgacgag gatacgccac cgccgcaggc cgtcacggga cggcgcgagg
      541 cctgcgccgg gaggtgaaga ggttgacccc cttcgccagc ttcggtacgc agaggaggaa
      601 gagccctccg acggcgaccg cgaggacgag ggcgacgaga gggaggaaga tcgacacgat
      661 cgcctgcagg gcggcgagga tgtcctcggc gagggacagg gcgaagttgg cgacgcccgc
      721 cgtcagcgcg gtggaggtga ggcgcacggt gcccttgacg acatgtgcgg cgagcgcgat
      781 cacgccgcct ccccctccgg ccagaaccgt catcgacgtc tcggcgcccg gtgcgacggc
      841 ggcgacggcg agaagggccc ctgccgccgg ccggacgacc gtgtggacga tgtcccagac
      901 gtggtcgagg cccgggatct tgtcggcgaa gaactcgagg acgtagagcg cgaggaggac
      961 cgacagcgcc gcgggctccg agaggagccg ggcgatcgcg cccgggaagt cctccgggaa
     1021 gaggcgggcg atcccgccgt agacgaggag aacggcgtag gcgttcagac ccgctcccgc
     1081 cgcgagaccc aggcccgttc cgaagtgcgc gatcgcctcg accaccgcgg gaggatagcg
     1141 cccgggaggg gcgctcagcg aggcttcgtc gccgcgatct ccgcgagcgc cttcgacgcc
     1201 tttcggttcg tcgggtcgag cttgaggact tccttgagcg ccttgacccg ccccgtctcg
     1261 ttccgctcgg cgccgagcgc gtcggcatac gccagccaga cctcgacgga atcggggagg
     1321 ttctccgtcg cccggcggag cgtctccgtg gcctgccgga tccacttcgg gttccgggcc
     1381 tggacctttg cgaggagcgt ccagtactcc ggccgctcgg ggtcgaactc gacggcctgc
     1441 cggagcatct cgtaggccgg gaagaagtcc tccatctcga tcagggtccg tgcccgctgg
     1501 tagttcgcct tggcgacctg cttctgcacc tcgcgcgtgg gctcgccgta ctgcacgggc
     1561 gcggacatcc ccccgctcgc ccgcagccgg tcgtactgct gccgcgacgt cgggctcgcg
     1621 aacgaccggt cggcttcccg gatcttcgcg aagagcgctt ccagcgcttc gccggcgtcg
     1681 gcgagcgtcg accggagggc gttgtcgggg tggaagagcc gcgcgcgctc gtggaccgcc
     1741 cgccggaggt ccgggtcggc cgcgtcctcg gcgactccga gcagctcgta ggcggtcgtc
     1801 cagtcgagcc tgcggtaggc gttccagacc gtcttcgact gctgctcgag gagctccgcg
     1861 aggcgcgggc tcggtggcgt cccggccacc tccacgttga ggctctggac gccggccggc
     1921 gcctgctcgt cgggcggcct cttccggtcg acgacggaga cgagcccgac ggagatcagc
     1981 cgctcgagga tcctcaggtc gaggcagacg tcggcggccc tcttcgtccc gtcgacggcg
     2041 tccagggcct ccgcctcctc cgccgtgacg acggcgcact ggtatcggag gaggaggtcc
     2101 ggcgagagga cgagcttcga ctggggatcg acccgtgggg cgaccccggc gaccggcggc
     2161 cgtcggcgga ggccctcgag gacgaggctc gccgtcgact ggtcgaaggc gacggggaga
     2221 tcgggcgccg acccgagcgt cacgacgctg aacgtaccgg cgctccactg gaacgacgag
     2281 tagaggacca tctcggcgag ggcgcgaacg caggcgtccg cgacgtaggg cgcgacggct
     2341 ccggcgtcgc gcagggcgct cgccagaccg cgcccgggat ccgcgagggc gcgttcgaag
     2401 gccgcgaaga ggacggactc gttcgcgagg ccgaacgtgc ggaggaggtc gccgacgtgc
     2461 tgcgcctcgc gattcgagga cgccgagacg agccgtcccc ttcggaacca gaagctcctc
     2521 tcctccccgc gatacgagac gaggacctgc aggtgcctgc gcgaggcgta ggcgctccgg
     2581 aggacgtcgg cgagctccgt cttctcgagc gtgccgcccg agagcgagcc ggttccgggc
     2641 ttcagcgtgt ccatgttccg gcgattctaa cggcggtcgc cgccggacga cgcgcggaag
     2701 cgctcgacgc gggcggccac cgccttcccg gcggccgcgg cgagggcctc cggggaggcg
     2761 gcgaggacgg cctcgccgtt cccgaagacc gtcaggagcc gcttcacaga ggccggaccc
     2821 agaccgggaa tcgcgaggag cgggctctgg agcgtcctcc gggagcgggc ccgccggtgg
     2881 tgagagagcc cgaaacggtg ggcttcgtcg cgggcccgct ggagcagctt caggccggca
     2941 ttccgccggg ggagcgtgat ggggagcgag cggcccggta cgaagagctc ctcctccctc
     3001 ttcgccagcc cgaccgcggg aagctcgatc ccgacccggt cgagcgcggc gagggccgcc
     3061 gacagctgcc ccttgccgcc gtcgatgacg agcaggtcgg gaagcggctc cccctcggcg
     3121 cgccggcgct cgtagcgccg cgtcacggcc tcggcgatcg acgcgaagtc gtcgggcacc
     3181 ggccgctcga tccggaaggc ccggtactcg tccttcgccg ccgtcccgtc gacgaagacg
     3241 acgcaggagg cgtaggtgtc gcttccctgg aaatgcgaga tgtcgaagca ctcgatgcgg
     3301 gacggccggc gatcgaggtc gagcgccttc gcgagggcga gcgtcgcctt ctcccccgct
     3361 tcgcgagtcg tccggaagcg ccgcacgtgg ctcgtacggg cgttgtcccg ggcgatcctc
     3421 acgcggtcga acgcggcgcc gcgcttcggg gtccggatgg cgaccttcgt cctccgccgg
     3481 tccgacagcc aggcctcgag cggctcgagc gcctcctccg cgacctcgag ggggaggtgg
     3541 acctcccgcg gcaggaacgt cgtcgcgtcg tagtactgcg acaggagcgc gacccagaac
     3601 tcctccggtt cgacctctcc gagcccttcc cagaagagct ccctcgagtc gaggaccgcc
     3661 ccgccgcgga cgtggaggac ggagaccgcg acgttcccgc cgtcggcgaa gtccccgaac
     3721 acgtcgacgt cctccccggc gagcgactgg acgcgctgct tctgcgccag ctcctccacg
     3781 gtccggaggc agtcccggta ggcggcggcc atctcgaact gctccgcccc ggccgccgcg
     3841 tccatcttcg cggcgaggcg cggcgacagc tcctcgttcc gtcccgagag gaagagaacg
     3901 acgtcccgga cggcgtcgtc gtacgccgcc ttcgtcgtca ggcccgcgac gcacgggccg
     3961 aggcaggcgt gcatgtcgtg gtagaggcag gggcgcggca gcgtgccgtc gatctcgatc
     4021 cggcaggtcc tcacctggaa catccgctgc accatcctca tcgccccgcg gcaggtgcgc
     4081 gccggcagga acggcccgaa gtagctgtgc ccgtccttca ggacgcgccg cgtcacgtgc
     4141 gcgcgcggcc actcctcccc cgtcgtcacc ttgatgtacg ggtacgtctt gtcgtcccgc
     4201 agcagaatgt tgtagcgggg cttgtgcttc ttgatgaacg cgttctcgag cgcgagcgcc
     4261 tcgctctcgg tgttcgcgac gaccgtgtcg atcgtctcga actccgcgag gagcgcctcg
     4321 gtcttcgggt gcttcggccc ggccgcgaag tacgacgcga ggcgcttccg gatcgaccgc
     4381 gccttgccga cgtagaggat cttccccttc gcgtcgcggt agaggtagat cccgggctga
     4441 tcgggatacg aagagaggtc gggagaggcg cccacgggag gattctaggg agccggcgcg
     4501 ccggccggcg tcgtcagagg gccttcccga gcttcgacgc gatggtcgag accttcacgt
     4561 agccgaagaa ccggccgctc gcgaggagac cgaggccgtc cgccacccgg gtccgggcgt
     4621 cgcgcgcgta gaggacgccg aaggccttcc gcatgaactg ctgaaaggcc ccgtcgtacg
     4681 ggtaccacca gggggccgac ggggaggcgt ggaggtccac gctgacgtgc ttcacgttca
     4741 cgagctcccg gaggccgtgg acgccatggc tccgcccgat ccccgacttc ccgagaccgc
     4801 cccacgaggc cgtcggctcg ccgaacgaga agaggtggtc gttgatcgtg accgtcccgg
     4861 cgcggagctc ctcgacgagc ttccgcgccg tccgcgtcga acgcgtccag ccggaggccg
     4921 tgagagcgaa ctccgagtcg ttcgagaggg cgatcgcctc gtcgagggag tcgacgaccg
     4981 cgatcggcag gaccggcccg aacgtctcct ccgtcatcac gcgcatcccg tggtggacgt
     5041 cgacgaggac cgtcggcggg aaccagttcc ccttcgcggc cggccgctcg cctcccgtca
     5101 gcgcccgggc cccctgcgag atcgcctcgc gcacctgccc gtcgacgaag tcccgctggt
     5161 cggcggtcgt catcggcccg aggtcggtcg cctcggcgag ggggtcgccc tgccggagcc
     5221 gccgcgtctt ctcgacgacc cgggagacca gctcgtcggc gatcttccgg tcggcgtaga
     5281 cgcgctcgat cgagccgcac gcctgcccgg cgttcgcgaa ggcgccccag acgagcccgc
     5341 tcgccgtcct ctcgaggtcg gcgtcggacg cgacgacggc cgcgtccttc ccgcccagct
     5401 cgaggacgaa cggggtgacg ttcccggcgg cccgccgcat cacgtgccgg cccgtctcga
     5461 cggagccggt gaacaggatc ttggcgatgg cggggtgctc gagcagccgg tcgacgtcgg
     5521 acccggtcgc cgggacgacg ttgacgactc cgggcggccg gcccgccatc cggcagagct
     5581 ccccgatcgc cagtcccgtc cgcggcgtgg ccgaggcggg cttgaggacg acggtgttgc
     5641 cggcggcgag gcacgcggcg acctccacca tcgggatcgc gaacgggaag ttccacggcg
     5701 tgatcaccgc gatgacgccg agaggctcga agcggtcgga ggccgccttg tgggcgaaga
     5761 ggagctgctc gtagtcgacc ggcttctccg cgaggagctc ctccgcgtgt cgggaaaggt
     5821 accggagcgc gtcggcggcg ggcacgaggt cgagggcgcg cgcttcggcg acgggcttcc
     5881 cctgctcggc cgccacgagc cgcgcgaggt catcgtgcga gcgccggacc gcctcgagga
     5941 gggcttcgag gtgccttcgg cgctccttcg ggctccgcgc cccccagtgg aggaaggcct
     6001 tccgcgccgc ggcgacggcg gcgtcgatct cctcggcccc tccgcgcctc acctcggcga
     6061 gcggggcccc ggtggccggg ttccgcgtga cgaacgccct ccccgtctcg acgtcgagcc
     6121 cgtcgatgat cggcccggcc ggcggggaga agcgcctcaa atcatcgctc cgcgcgtgat
     6181 cttctcgacg tgcctgcgat agaaggcgac gaggttctgc acgtagtcgg tgaagggcgg
     6241 gcaggagacg tcgaaccgtg cgaggtcggc cgcggcctgg gacgagtcgt agcgggccgg
     6301 gtggtcgaag tactcgacgg tgacggccgg catcccgaac cagtcctgga tcggcttcgg
     6361 cgagaagagg agcttcgcga ccacggccgg gacgggaaca tagacgaact gcttgccgag
     6421 cgccttcgcg aagagctcct cgatctcgaa cgcggagagc ggtcgcgggt cggtgaggtg
     6481 gtacgtcttc ccgatcgacc cctcccacgt cgagaggcgc gcgatcgcct cgaggacgaa
     6541 gtcgacgggg acgaggttca ccgggttcgt ccccgtgccg accttcacga agacgccggg
     6601 cgacgggagc tggcgcatcg cgttcagcgc gaagtaggga ccgtcgaact tcgccgtctc
     6661 gccggtcctc gagtcgccga cgacgatggc ggggcggtag gtggcggtcg ggagagcgct
     6721 ccctctcacc gcctcttccg ccaggaactt cgtctcctcg tagtagttct tgaacgactg
     6781 ccccacgtcg aggtccgtct cgcggaagag cccgacggcc gtgcccgaga cgtaggccgt
     6841 ggagacgtag tcgaagcggt gcaggtgcgg acactcctcg aggaacgcca ggacgttccg
     6901 ggtcccctcc acgttgatcc gcatcccgac ctcgcgcggg accgcgaggt cgtagacggc
     6961 cgcgaggtgg tagcagccgg tcagcttgcg gtggaggact cgggcctccg tctctccgat
     7021 cccgaggccc ggcgccgtga tgtctccgac gacgagctcg atccgtcccg ccgttcccgg
     7081 gtgcgcctcg gccagctggg cgaccgagga gcgcgcgaga tcgaggaact tctcctgcac
     7141 gagacagtgg aagagggtgt cgggctcgag ctccaggagg cgcgggatga gccgcgcgcc
     7201 gatgaagccc gggaacccgg tgaagaggac gactcgggac ggggcggggt tcgcgctcgc
     7261 tttcttcctg gccataccgt gcctctcttc ccgacagtct agagcgattt ctgcaatgcg
     7321 gcgtcggtcc ggagcctctg ccgaaacgcc tcgatcgccc ggagcatcgc gggaggccgg
     7381 gcgcactcct cgccgtcgag ggagaggacc ggcgccgcgg tcgtgagcga gccgaggagg
     7441 aagctccccg cgaggagctc ctcccggccg actcccctcc gctcccccgc gccttcgagg
     7501 aacgcctcgg ccgtcacgga agggaggcct cccgggccga gcccggcgag gcggcccccg
     7561 ttccaggcga cgagcgccgt cgagctcccc tccacgtaga cgtcgccgtc ccggaagagc
     7621 gcctcgtcgc accccgcctt ccgggcgtgc ctgagcgcgg cgaccgcgcc gaagtagctc
     7681 gtcgacttga agccggacga gtcgcgtcgg aaccgctcgg gcgccgtcag cgcccgggag
     7741 ccggcgcgcc gcgccagggt gtttggcgga atgggccgca ccgacgcgtc gagcctccag
     7801 gaggccggct cctccacgtc gtggccgatg gcgatccagg agagccgcag ggagcgctcc
     7861 tcgtccccct ccgcgatccc ggcgagggcg gagcgcgccg ccgattcgac ggcgtcgccg
     7921 gagggggccg gcagctcgag cgcctcgagc gtcgcgagca gcctgcgcag gtgcgcctcc
     7981 cagagaacgg cgcggcgacc gacgacgagg agcgtctcga agaaaccgaa gccgcgctcg
     8041 acgacgggcg cgtcgtaggg gatcgggtcg gggctcatca cacctcgctc tcgcccgccg
     8101 ccgcggcgag cgcccggaag aactctcggg acttcgtctc ggattcctcc cactcgtgcc
     8161 gcgggtcgga gtcccagacg atccctcccc ccgcatggta ggcgaccgcc ccccccgaga
     8221 cgatcgctgt ccggatcgcc acgttcagga cggcggtccc gtcgccgcgg acgaaaccgg
     8281 cggcgccggt gtagggcccc cggggcgcgg gctcgagcct gcggatgaat ccgagggccg
     8341 accgcttcgg cgcccccgtg atcgacccgg ccggaagggt cgcgaggagg aggtcggaga
     8401 gacctgcccc cggcgcgagc gatccggcga ccgtcgactc caggtggtga aggtggcggt
     8461 agctcttcac gcggcgcagg gcggggacgt ggacgcttcc cggccggcag acccggccga
     8521 ggtcgtttcg gacgaggtcg acgatcatca ggtgctccgc ggcgtccttc tccgacgaga
     8581 ggagcagcgc gcgggccgcc gcctccgagc cgccggccgt ccgcgcgacg gtccccttga
     8641 tcggggccga ggcgacgcgg cgggacgccg ggtcgagctc caggaagagc tcgggggagt
     8701 tggagacgac ggcggccgcg cccgtcgcga cgagaatcgc gtagggcacg gggttgtccc
     8761 cgtacaggag gtccgcgagg cggcgcggcg ataccgggag cgccctcacg acgcggcgcg
     8821 tcaggttcgc ctggtagacg tctcctctct cgatgccgga ccggatctcc gacacggcag
     8881 cggaccactc ctcgagcgtc atctcgccgg cagggccgaa cggtccttcc cgaccgtctc
     8941 ctctcgcgaa gtccggtcgg atggagggcg cgtccgcaga gcccgcgcgg cgcaccgcgt
     9001 tccgcaggtc gaagagcccg aaccaggcga ggggtgtcgc cggaggccgg aagagggccg
     9061 tggatccttc gagcgcgacg ccggcctcgt aggtcaggaa gcccgccgcg aagagctccg
     9121 ggcggcccga ccgcgccacc gcgtcgagcc gttcgaggag gtgcggcagc tcctcggggc
     9181 ggtccgcctt cacatcgagg acctcgaaag ggtcgcggag ctcgagcggc tccgggagct
     9241 tccacccggg accccgcaga agaacgaagg gcgacggcac gctcgggtag tctacggagc
     9301 gtgagcgagg aacgccgggt cgagaccgag gtgaaggtcc gctacgccga aaccgacgcg
     9361 cagggagtcg tctaccacgg ctcgtacgtc gtctggttcg aggtcgggcg gacggagtat
     9421 tgcgaggccg ccggataccc ctacccgcgg atggaggcgg agggggtaca gatcgtcgtc
     9481 gttgatctct ctgcccgctt ccggcggccg gcccgctacg gcgacgtcgt gcgcgtcgcg
     9541 acccgcctcg ccgcgatccg gagccgcggc tgcacgttcg cctaccgcgt cttcctgccg
     9601 gacggcggtc tcgccgtcga gggcgagacg gtgcacgtct tcgtcgacgc cgccaccggc
     9661 cggacgatcg cggcgcccga gccgatccgc tcggctctcg ccgccttcgc cgcgggctga
     9721 ggcctcagct ctccttgccg ttcggcagct tcttctggag cgctcggtag acgagcgagg
     9781 ggacgagctc cgagacgttc cctccgagcc ggtggacctc cttgacgagg cggctcgaga
     9841 ggtacgagag ctcctccttc ggcgtcagga agaccgtctc gacctccggg tcgaggcgcc
     9901 ggttcatgag cgccatctgg aactcgtact cgaaatcgga gacgacccgc agcccgcgga
     9961 ggacgatgtt cgtcttctcg gcgcggagga attcgacgag gaggccggag aaagccttca
    10021 ccttcacctt cggctcgcgc cggaagacgt cccgcgtcat ctgcacgcgc tcctcgacgg
    10081 tgaagagcgg ggccttctcc gtgttgcaga ggacggcgac gaggacgcgg tcgaagatgt
    10141 ggcagccccg gcggaccagg tcgacgtgcc cgttcgtcat cgggtcgaac gagcccggat
    10201 agacggcgat tcgcatgaga tgaggggcat tctaggagac ggacggggaa agtgctcgcc
    10261 gctggcgtga accggggggg ccggggggcg gcgtctcgcg aggccgatgc tgacgagaga
    10321 gcgatcacgg ctttgcgtga gctggggggc ccggggggcg gcggacagcg ccccccgggg
    10381 agcgcgcccg ccggtgagcg cgccccccgg cgagcctagc ggctttcgga gagcgcctct
    10441 ccggccttcc gcgccgagag gacgaggagg aagagcgcgg ctgcgggcgg gaggacgcac
    10501 caccatgcgc tcgtcaggct ctccctcgcg tctgcgagcg cccgccccca ggaggccgtc
    10561 ggcggcggca cgccgaggcc caggaaagaa agcgacgcct cgaggaggac cgccccgccg
    10621 acgacgtaag ggagcgaggc gatcgcggga gcgatcgcgc cggggaggac gtgccggagc
    10681 acgacgcggg cggggctcgc gcccgccgcg cgggccgctt cgacgtaggg cgaggcggag
    10741 aggcgaagcg cctcggccct cacgagccgc gccgcctcgg gccaggccgt cagcgcgatc
    10801 agcagcgcgg ccgtccagcg cgacggcggg gccatcgctg cgccggcggc gacgaggacg
    10861 agggccggaa aagcctggac caccccggtg accacgagga cggcccgatc gactcccgga
    10921 ccgagcgtgc cggccgcggc gccgaggaga gtccccgcgg cgaacgcgag ggcggccgcg
    10981 gccagaccga cgaggacgga gacgcgtgcc ccgtggagga ggcgtgacag gacgtctcgt
    11041 cccaggtcgt cggtgccgag gaggtgggtc ccgtccggag ggcgcaggcg ggtcgcgagg
    11101 tcggtcgcgt cggggtcgaa cgggacgggt gtcagcgccc ggatcgcctg cggcgcgtcg
    11161 gcggggagcg ctcgagccag gagcggcgcc aggagcgcca gcgccgggaa gagcgccagg
    11221 acgagcaggc gtcgctggac tcggccgcgc gtcacgagtc ctccgcgtcg acgaggcgcg
    11281 ggtcggcgag cgccgcgagc gcgtcggcga ggagcgtcgc gaggacgacg gcggcgcccg
    11341 aggcgagcgt gaggccgagg accgtcggca ggtcgcgggc gaagaccgag tcggccagga
    11401 gctgcccggt ccccgggacc gcgaagaccc tctccacgag gacggagccg gcgacgagct
    11461 gcgggacgag cgccgcgagg agccccgcga aggccggtac ggagcggcgg agcgcccgga
    11521 gcgtctcctc ccggggcgac tccccgcgcg cccgccccgc gatcgccggc ggcgaggcaa
    11581 gcgcggcccg cacgctctcc ctcaccacgc gggcgatcgg cgccagggcg acgagcgcga
    11641 gcgtgaggga tggcagcgcg aggcttgcga gaggggacga ggcgtccgcg aagaccgggg
    11701 tccagcggag cctcacggcg aagaggaaga ggaggaggag gccgagggcg aagctcggaa
    11761 gcgagaagag cgcgtcgagg aggagcccgg cgagccggtc ggcccgacct cccggacgcc
    11821 gcgccgccgc gacgccgagc ggcacgcccc cgagggccgc gaggaggagg gcggtgccgt
    11881 tgacggcgag cgtcgccggg agcgtctctc cgatccgctc ggtcaccttg cggccgtccc
    11941 gaaaggagcg accgaggtcg aggcggaccg cagagccgag ccaggcggcg agacgggcga
    12001 cgaccgggcg gtcgaggccg tggacggcgc ggaacgcctc gagcgcctcc gccgacgccg
    12061 gccggcgtcc cggcatgccg cctgcggcca ggcgggccgg gtcccccggc gccgccgaga
    12121 ggaggaggaa gaccgcaacg aggacgccga cgagcgtccc gaccgcgccg agcgcgcgcc
    12181 gcgccaggaa ccgggtcacg gccggagatg ttacgcggag gcggcgccgt cgcccgtcag
    12241 atcggcttcg acccgcgcgc cggttgccag gaggccgctc cgggccagaa gagccagaga
    12301 ccgatcggcg agctcgtcac gttctcgatc cggtcgcgca cgccccacgc gaccgagacc
    12361 gtgtgcagga acgtgaacgg ctggtcctcg gcgatcaccc tctgcagctc ggcgtagagg
    12421 acgcgcgccc tctccctgtc gaacgtcccg gcgatctcgt ccatgagcgc gtcggccctc
    12481 gggtttcggt agaacgcgtg gttcatgccg ctgggcggaa cctgcgagga gtgccagttg
    12541 ggccgcaggt cggggttcgg gtcgaggttc agggaaagcg agcaggcctc gtagtccccc
    12601 gtgtcgacct tctcgacgaa cgcgccccat tccatcggcg agagcttcgc ctcgatcccc
    12661 gcctcgccga acgcccgctg cgcgagctcc atgacctgcc gctggcgctc gctcgcaccg
    12721 ccgaagacga gggagaactc gagccggacc ttccctttcg cgcgaaggcc gtccttgccc
    12781 ttcttccatc ccgcctcgtc caggagggcc gcggcggcgg ccgggtcgtg agggatgggc
    12841 cggagcgtcg cgtcgtgggg ccagaggccc ggcggcagcg ggccgttcgc cggcttcgcg
    12901 aggccgccgt agagctcgcg ggcgatcgcc tcgcgatcga ggagcatcgt cagggcgcgg
    12961 cggacacgcg cgtcggagaa ctgcggaagc cggttgttcc aggcgatgta ggtgtaggcg
    13021 atctcgtcgt agaccgtctc gcggatcgcc cccgccttcg cggccagcgg acgctgggtc
    13081 gcattcagcc gcgtctcgtc gagcgcgccg gtgaggaggc cctggaaggc cgaggccgac
    13141 tcggggacga cccggaggac gacctgctcg tacgcgggag ccgtcccgaa gtactgcgtg
    13201 tttcggacga gtcgaatcgt cggcccctcc cacgaggcga ggcggtacgg gcccgtcgcg
    13261 agcggcctcc ggttcgccgg gttcgtcgcc acgtccgtcc cgcggtagac gtgggccggc
    13321 aggagcggca tgttgaaggc gtcgcgctgg gcggcgtagg ccttccggaa cgtcgcacgg
    13381 gccgtccgcg gatccagcac gtccaccttc tcgagctcgg cgaagaggct ccggcggtag
    13441 agggccgggg tcttcgggtc gaggagcgcc tccagcgtca ccttcacgtc ctcggccgac
    13501 acgggcgtcc cgtcctccca gcgggctccg ggccggagcc ggaccgtgta gacgaggcgg
    13561 ctctcgtccg gctccaccga ctcggcgaga ccggggacga ggtcgaggtg ctcgtcgtag
    13621 tcgaggaggt tccgctggag gagcgcgatg acgacgttct ccgggtccga cgtcgccgtg
    13681 agcgcgttca gcgtctgcgg ctccccttcg agccgccggt gaaggacgag gctctggtcc
    13741 ttgccgacgg gacctccgca gccggaaagg aaaagggcga ggacgaggag ccgcttcacc
    13801 ggagctcctc cgggtactcg aaggagatcg aagcgccgtt cccggccgcg tcgacgacgc
    13861 ggatggcgag gaacgccggg ccgtcgggct tcggtaccgt cagcgtcagc tcctcctcgc
    13921 gtccgtcgag cgtcccgtcg gccggcgtga ggggccgcca ccggtcggcg gagaccgtcc
    13981 cctccgcgcg ggtgatcggc gacagagcgt cgacggcctt cacccggagg acgacgcgac
    14041 ccccctccag ccgcgccccc gtgcgaacga tcgacggtgg cgtgccgtcc acgagcgcca
    14101 cgtcgctctc ctcgctcgtc tcgagcgcgt ccccgtcgag gttcgccgcg cggtccgacg
    14161 cggtgacgcg gaaacggtag cgtccgtcgg gaaggggcgc cgtgtcgaag gagaagaagg
    14221 ggtcctcgag gtccctgcgg atcgggaacc aggtcacgga gccctctctg cggacctcga
    14281 ggtcgtagcg gagcgcgtct ccgttcgggt cggtcccctt ccaggtgacg gtccggaagc
    14341 ccttccggaa gagcttcttc cccggcgtct ccgccggctt ctcgggctcg aggccggcga
    14401 acgcgccgtt ctcgtcgggg ttcgtcaccg agaggacggc cggtccggag gcggcgccgg
    14461 agcgggcgaa gactgctccc
//
LOCUS       DAOTEZ010000057        14247 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2946_length_14247_cov_6.850972, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000057 DAOTEZ010000000
VERSION     DAOTEZ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14247
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2946_length_14247_cov_6.850972"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="P5164_04630"
     CDS             complement(<1..817)
                     /locus_tag="P5164_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43213.1"
                     /translation="MSARVSLLVPLLLLAASAPGAEPPFRAELSVGFTRDAIATERPL
                     VVLDGTPAAGYDYSADEEVRTLDVAARRWFGPVPDDGATPLPLLPYVARVSSASARVA
                     VAGASRDSFGRASGQLATYEVWYAGDGSLREAELSGEWFVGRGLALRGGLGHGRERET
                     AASTSLESPSGRADVGTATTRAKVTSGTLGAALRLGEHEVSVSGAYGASDFSREDATV
                     FTGGVQPLFSRLTTDGVTRRGRVAARLVLLRRRLAVDLSGEYALATSSSDLDTT"
     gene            complement(873..1904)
                     /locus_tag="P5164_04635"
     CDS             complement(873..1904)
                     /locus_tag="P5164_04635"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HRY43214.1"
                     /translation="MTHSGGVLAALALAALAAVGCSVSGRRPATFAEAAREKADVTIV
                     VTDSGLGGLSVVADLASRLPSSGVVRSARIVFVNALLDDAIGYNDLRHEADKVRVFDA
                     ALDAMERRYSPDLILVACNTLSVFYERTLHAKRGATETVSIVPMGVDLVEKALRERPE
                     ARAVLFATKGTIDSNAHRRLLEQRGIAADRIVGQACPKLTGAIERGAHDPETASRIEG
                     FVKEAVGRLGDRPGPVVASLNCTHFGYARPLWEEAFEKLGHPAVTVLDPNSLMTDHVL
                     RAGGERRYPETAVTVEVVSKTPIGEDVKGPLGALLGTVSPATADALARYVHVPDLFHV
                     EVDPAAILR"
     gene            2099..3103
                     /locus_tag="P5164_04640"
     CDS             2099..3103
                     /locus_tag="P5164_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HRY43215.1"
                     /translation="MSSGGADRARPGRPEDVPPSYLDSSSRPDLLAGGIRMIPVETPA
                     GPFRVWTKRVGNNPRVKLLLLHGGPGFTHEAFEACDTRLPAAGVEYYYYDQLGSYYSD
                     QPDRPELWELPRFVDEVEQVRRALGLGKESFFLLGQSWGGILALEYAFRYQEHLKGLV
                     VSNMMSSVPAYNAYARDVLAPRMDPAALDEIRRLEEEGRTGEARYMELLGPHYYEEHV
                     LRIPMDEWPEPLARSFRHMNPKVYVPMQGPSELGASGKLERWDRSADLGAIRVPTLVL
                     GAAHDTMDPEHLRWMAGAVARGRYHHCPEGSHLAIYDDQERYFEGLISFLRDVDEGVF
                     "
     gene            complement(3100..4302)
                     /locus_tag="P5164_04645"
     CDS             complement(3100..4302)
                     /locus_tag="P5164_04645"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY43216.1"
                     /translation="MNGAGPPGPPEATGRELFAAGFVTFSLLYETQPVLPLLASEWGL
                     SPAAASLSLSSATLSLAFGMLAWALLSDAKGRVGVLRVAILLAACLGLATPFAPSFPL
                     LVALRGLQGLALAGVPAVAMAYLSEEVAPRRSGAAMGLFIAGNAVGGMVGRILVGLLS
                     ARLGWRGGLLVLGLVDLLLALWFARTVPPSRRFRPRPLTRSALRSGLASSARDPVLLG
                     LYATAFLVMGSFVTVYNYLAFSLQGPPWSLAPSTVSWVFLLYTVGVAASPLAGRLSDR
                     AGRPRVLLAGVACQLAGAVGTLLVPLAPKLLALALFTLGFFTAHAVASTGVARRGGAE
                     KAAASAHYLLAYYAGSSAVGTLGGVVLARAGWSGVVGMVAALLGAALAVAAALGRTSE
                     SGREEAAT"
     gene            complement(4322..5365)
                     /gene="ltaE"
                     /locus_tag="P5164_04650"
     CDS             complement(4322..5365)
                     /gene="ltaE"
                     /locus_tag="P5164_04650"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HRY43217.1"
                     /translation="MRTIDLRSDTVTRPSAAMRRAMAEAVVGDDVFGDDPTVRALEEH
                     AAALFGKAAGLFLPSGTMSNQAALLAWTERGDEVFVHEQSHVLLYEQAGAAVLASLQT
                     RSFASDDGMLDPGAMEDFVHTGEDPHNARTRLVALENTHNHCGGRVLDSRGVLAVRAF
                     CDRHGLVLHLDGARVCNAAVASGRPLSEIAAPFDSVSVCLSKGLGAPVGSVLVGPADF
                     VRKARRARKLLGGGMRQAGILAAAGLFALRENVARLAEDHRRCRTLAEAIAKAPGLSV
                     DLARVETNMVFASTRLTGTKAADVARRLAEAGVLALDESPWLLRFVTHLDVDDSDVDE
                     AGEIVTRTMSAIA"
     gene            complement(5428..6225)
                     /locus_tag="P5164_04655"
     CDS             complement(5428..6225)
                     /locus_tag="P5164_04655"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRY43218.1"
                     /translation="MSERTELLPGSFCWPELATPDSAKAKAFYAGLFGWEAADVPTAG
                     GAYTLLKLRGKDVAALRTLSGPELAQNVPSHFMTYVSTASVDASAARAAELGGKVCHG
                     PFDVEGVGRMAVVADPEGVVFALWEARGHIGAQLVGEDHTLCWTELVTKDPDGAKAFY
                     GGLFGWTWKPSETSEVEYHETYRGDQAIGGLLPMKGDWWGDVPPHWMPYFLVPDCAAA
                     ARRAGELGGGAAVPPTEIPHVGTFAVLRDPLGAHFSVIAMAARPEGA"
     gene            6384..8120
                     /gene="ade"
                     /locus_tag="P5164_04660"
     CDS             6384..8120
                     /gene="ade"
                     /locus_tag="P5164_04660"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HRY43219.1"
                     /translation="MAEGKLPLAAFVDAARGSVPADLVFRNARVVNVLTGEIHEETVG
                     VAGDRVLGFGPYRAARPENEIDLKGAFLAPAFWDAHIHVESTMTTPPVFARLAAAHGT
                     AAVVTDPHEIANVRGAEGLEAFLASADGLPVELHVMLPSCVPATHLETAGADLGPADL
                     ARFYGRKNVLGLAEMMNVPGFLFGDPNVRAKVEDAASRGLAIDGHSPLLSGLDLNAYV
                     GAGIRTDHECLGREEAAEKVRRGMSIWIREGTAAKNLDDLLPLVNATNSERFAFATDD
                     RHPWDLLTHGHVDHHVRRAIALGLDPVLAFRLASLSAARHYGFRDRGAVAPGYRADLI
                     TFESLDDVRPDLVVLGGRRVAQGGKLLVDVPSRGIPEGPSFRVDGFDASRLRLPAEPG
                     ARVRVIEVHPGSLATGASTAEAHVVDGAAAADPSRDLAKLAVVERHRGTGNVGVSFAR
                     GFGLARGAIASSVAHDSHNVVVAGCDDASMETAVRRAAEIGGGIVVALGAQVLAEVAL
                     PVAGLMSNAPMEEVDRAERRANEATRALGTAVDHPFMTLAFLALPVIPELKLTDKGLV
                     DVTRFEHVSVFA"
     assembly_gap    8156..8255
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8317..8757
                     /locus_tag="P5164_04665"
     CDS             8317..8757
                     /locus_tag="P5164_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43220.1"
                     /translation="MTAVGVLAFLSVKLVAYAAWCRLGLGLLAPELPAAALRRAGFLA
                     FVRLAIGLLLGWLLLLGLATLVPAPNRLGVPPLALLAGSALLRWLEWSFVGALAAGRA
                     RSPRAVLLGRSGKEHLWRVGGVALSFATDLAGLLGAGALGVIPC"
     gene            8790..9242
                     /locus_tag="P5164_04670"
     CDS             8790..9242
                     /locus_tag="P5164_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008985660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRY43221.1"
                     /translation="MKRIRHSITIRASRDAVWAAMLGPETYRVWTTPFCEGSYYEGSW
                     NTGDRIRFLGPGGEGGMTSVIAESRPPEFVSIKHLGIVKDGVEDTTSPAATAWAPSFE
                     SYTFVDVGGATEVTAELETPPDFEAFMDDAWPKALAKLKEICEESASR"
     gene            9326..10249
                     /locus_tag="P5164_04675"
     CDS             9326..10249
                     /locus_tag="P5164_04675"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007076180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HRY43222.1"
                     /translation="MNVLDSIGSTSLVRLRKVVPAGSADLLVKLEGENPTGSMKDRMA
                     LAVISRAEADGRLRPGGTVVEYTGGSTGTSLAFVAAAKGYRIRIVTSDAFSREKRDHM
                     AALGAELTLVPSEGSLTTKKLILDMIAAAREIAREPGTFFTNQLENADAIAGYVPLAE
                     ELLEQTGGEIDAFAHSVGTAASLRGIAAVLKGRRPGLRIVAVEPAESSVLLGSPAGPH
                     KIEGVGVGYVPPLWEPSLVDEVLAVATEEAKEMTRRLAREEALFAGTSTGANVVAALR
                     VAARLGAGATVATLACDTGLKYLSTDVYRRS"
     gene            10321..11358
                     /locus_tag="P5164_04680"
     CDS             10321..11358
                     /locus_tag="P5164_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HRY43223.1"
                     /translation="MITFFTPLHEGHAPRYEYFRGERVPCFESPVRARYVHDALVAAG
                     HELRSPTVDGAPLLVRVHSPRYLAFLESAWSQWLARDPANAAVQPFPSVWPVRTLRSD
                     VEPDDFVARLGLYSMDNGTPLAEGTWAAVKAAADAAASAAAVVASGVRCAFSATRPPG
                     HHAGADFMGGYCFLNNAAVAAEALLGSGSKRVALLDVDYHHGNGTQSLFYGRSDVFFA
                     SLHADPRFEYPFYLGHADETGEGAGEGFNLNLPLPAGTRGPEWLAALETACARAAAAG
                     ADALVVSLGLDTFAGDPLTRFALERDDFRRLGERLARTGLPTVLVLEGGYAAAELGAN
                     AASVLEGFEGA"
     gene            11473..12057
                     /locus_tag="P5164_04685"
     CDS             11473..12057
                     /locus_tag="P5164_04685"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRY43224.1"
                     /translation="MHSDLASTLADSRSATTRWLALVEPLSSTQANWRPAPDRWSIAQ
                     VLDHLAATTGPLAPVLLEAGAKARSAGRFSDGPFRYGPLARWLLRSLAPAGARPLPAP
                     KLYRPSASALDLAEVTRRFLEAQRAFESAAESAEGLDLARVRVPSPALRLFRLPLGVW
                     CLSTAAHTLRHLAQAERVRADSRFPEDRPDRGQA"
     gene            12138..12668
                     /locus_tag="P5164_04690"
     CDS             12138..12668
                     /locus_tag="P5164_04690"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRY43225.1"
                     /translation="MSSVRPVSGTYSLHFAPYIALVTEPDVLAVLETQGAAFEPALRR
                     LPAAAARARYAEGKWSVAQVVGHVVDCERVFGYRALATARGEKAALPSFDEDAYAAAA
                     GHDDCPLVELLDELAALRRSHVLMFRHLDGAAWDRLGLVGGHPNAARAWAFVMAGHLR
                     HHANVLRERYGVAVEA"
     gene            12778..13458
                     /gene="can"
                     /locus_tag="P5164_04695"
     CDS             12778..13458
                     /gene="can"
                     /locus_tag="P5164_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HRY43226.1"
                     /translation="MKHLPNLFENNRAWADGLRRDDPEFFTRLCRQQAPPYFWIGCSD
                     SRVPANEIVGLLPGELFVHRNVANLVVQSDLNALSALQFAVDVLRVKHVVVCGHYGCG
                     GVLAALRGERHGLVDNWLRHVADVREKHDAQLGRLGPERAVRDRLCELNVVEQVRNVA
                     STTVVRDAWARGQELSLHGWVYALEDGLLRDLGVSAKGEAELAEAYARAVAGRPDGVA
                     PPAGERGP"
     gene            complement(13466..>14247)
                     /locus_tag="P5164_04700"
     CDS             complement(13466..>14247)
                     /locus_tag="P5164_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43227.1"
                     /translation="ILGKELFHALVVDLDYPGSRLRLHDAASFRYAGPGRKVDLLPAE
                     DGHKSLRISIEGDAPVVVGLGTGQGGALSVFRHYADARRLLEGRPVSERRGGGVGGAT
                     LSKLATLRSVSVAGYDLRNVPATFQTTDVGGAVDTKRQEGNLGAEILARFRVLFDYAR
                     SALWLEPGPGLAAPFPKDKTGLDLDWADGALVVEHVAPGSPAAATGWKEGERVTALDG
                     EPAGPESWRTEVRWSEAPAGTKVRFTLADGTERTLALAEYY"
BASE COUNT         1844 a         5418 c         5044 g         1841 t
ORIGIN      
        1 gcgtcgtgtc gaggtccgac gaggaggtgg cgagggcgta ctcccccgag aggtcgacgg
       61 cgagccgcct ccggaggagg acgagccggg ccgcgacgcg cccccggcgg gtcaccccgt
      121 cggtcgtcag gcgggagaag agcggctgga ccccgccggt aaagacggtc gcgtcctcgc
      181 ggctgaagtc ggaggcgccg taggcccccg acacgctcac ttcgtgctcg ccgagccgga
      241 gcgcggcgcc gagcgtcccc gacgtcacct tcgctctggt cgtcgcggtc ccgacgtcgg
      301 cgcgccccga cgggctctcg agcgacgtcg aggccgccgt ctcccgctcc cggccgtgcc
      361 cgagcccgcc ccgcagcgcg aggccccggc cgacgaacca ctcgcccgag agctccgcct
      421 cgcggaggga cccgtcgccg gcgtaccaga cctcgtacgt cgcaagctgc cccgaggctc
      481 gcccgaacga gtcgcggctc gccccggcga ctgcgacgcg ggccgaggcg ctcgagacgc
      541 gcgcgacgta cgggaggaga gggagcggcg tcgccccgtc gtcgggcacg gggccgaacc
      601 agcgcctcgc cgcgacgtcg agcgtccgga cctcctcgtc ggccgagtag tcgtagccgg
      661 ccgcgggcgt cccgtcgagg acgacgagcg gccgctcggt cgcgatcgcg tcccgggtga
      721 agccgaccga gagctccgcc cggaacggcg gctccgcccc gggcgcggac gcggccagca
      781 ggaggagcgg cacgaggagc gagacgcgag cggacattcc gtccgaggtt agccgatgag
      841 ccgtcccggc ggccgggcga ggaacgatcc tttcagcgga ggatcgcggc ggggtcgacc
      901 tcgacgtgga agaggtccgg cacgtgcacg tagcgcgcga gggcgtcggc cgtcgcggga
      961 gagaccgtgc cgaggagcgc accgagcggc cccttcacgt cctcgccgat cggcgtcttc
     1021 gagacgacct cgaccgtgac cgccgtctcc gggtaccgcc gctcgccgcc cgcgcggagc
     1081 acgtggtccg tcatgaggga gttcgggtcg agcaccgtca cggccggatg cccgagcttc
     1141 tcgaacgcct cctcccagag cggccgcgcg tagccgaagt gcgtacagtt caggctcgcc
     1201 acgaccgggc cggggcgatc gccgagccgg ccgaccgcct ccttcacgaa gccctcgatc
     1261 cgggaggcgg tctccgggtc gtgagcgccg cgctcgatcg cgccggtcag cttcgggcag
     1321 gcctggccga cgatgcggtc cgcggcgatt ccccgctgct cgaggaggcg gcggtgggcg
     1381 ttcgagtcga tcgtcccctt cgtcgcgaag aggaccgccc gagcctccgg ccgctcccgg
     1441 agcgctttct cgacgaggtc gacgcccatc gggacgatgc tgacggtctc cgtcgctccg
     1501 cgcttcgcgt ggagcgtccg ctcgtagaag accgagagcg tgttgcaggc gaccaggatc
     1561 aggtcggggg agtaccgccg ctccatcgcg tcgagcgcgg catcgaagac gcggaccttg
     1621 tccgcctcgt ggcgcaggtc gttgtagccg atcgcgtcgt cgaggagcgc gttcacgaag
     1681 acgatccgcg ccgagcggac gacgccgctc gaggggaggc ggctcgcgag gtcggcgacg
     1741 acggagaggc cgccgagccc cgaatcggtc acgacgatcg tcacgtcggc cttctcgcgt
     1801 gccgcctcgg cgaacgtcgc cggcctccgg cccgagacgg agcagccgac ggccgcgagg
     1861 gcggccaggg cgagcgctgc gaggacccct ccggagtgcg tcatcgactc ctcccgcggc
     1921 cggcagccgc cccgcggatt ctggacccgg cggcgacacc gcgcgaacgg gtccgatctc
     1981 cccctcgcct gcgcggcgtt cctgccgtat cgtccgctgg attttccatc gccaggatcg
     2041 tcggccccgt cctcccgccg ccgggcgcag gacggggccg gctgacgaag gaggccccat
     2101 gtcgagcgga ggagcggacc gcgcgcggcc cggcaggccg gaggacgtcc cgccgagcta
     2161 cctcgactcc tcgagccgcc cggacctcct cgccggaggg atccggatga tccccgtcga
     2221 aactcccgcc gggccgttcc gcgtctggac gaaacgtgtc ggcaacaacc cgcgggtgaa
     2281 gctcctcctc ctgcacggcg gcccggggtt cacgcacgag gccttcgagg cgtgcgacac
     2341 ccggcttccg gcggccggcg tcgagtacta ctactacgac cagctcgggt cgtactacag
     2401 cgaccagccg gaccggccgg agctctggga gctgccgcgc ttcgtcgacg aggtggagca
     2461 ggtccgccgc gccctcggcc tcgggaagga atcgttcttc ctcctcgggc agtcgtgggg
     2521 cgggatcctc gccctcgagt acgccttccg ctaccaggag cacctgaagg ggctcgtcgt
     2581 ttcgaacatg atgtcgagcg tccccgccta caacgcctat gcgcgcgacg tcctcgcgcc
     2641 gagaatggac ccggccgcgc tcgacgagat ccggcggctc gaggaggagg gccggaccgg
     2701 cgaggcgcgc tacatggagc tgctcggccc ccactactac gaggagcacg tcctcaggat
     2761 cccgatggac gagtggcccg agccgctggc ccgttcgttc cggcacatga acccgaaggt
     2821 ctacgtcccg atgcaggggc cgagcgagct cggcgcctcc gggaagctcg aacggtggga
     2881 tcgctccgcc gacctgggcg cgatccgggt cccgacgctc gtcctcggcg cggcgcacga
     2941 cacgatggac cccgagcacc tgcgctggat ggcgggggcc gtcgcccgcg gccggtacca
     3001 ccactgcccc gaggggagcc acctcgcgat ctacgacgac caggagcgtt acttcgaagg
     3061 gctgatctcc ttcctccggg acgtcgacga gggcgtcttc taggtcgctg cctcctcgcg
     3121 tccgctctcc gacgtgcgtc cgagggcggc cgcgacggcg agcgcggcgc cgaggagcgc
     3181 ggcgaccatg ccgacgacgc cgctccaccc ggcgcgcgcg aggacgacgc cgccgagcgt
     3241 cccgacggcg ctcgacccgg cgtagtacgc gaggaggtag tgcgccgagg ccgcggcctt
     3301 ctccgctcct ccccgccggg cgacgccggt cgaggcgacg gcgtgggccg tgaagaaccc
     3361 gagcgtgaag agggcgaggg cgaggagctt cggcgcgagc gggacgagga gcgtcccgac
     3421 ggccccggcg agctggcagg cgacgcccgc gaggaggacg cgcggccgcc ccgcacggtc
     3481 cgagaggcgc ccggcgagcg gcgaggcggc gacgccgacg gtgtagagga ggaagaccca
     3541 cgagaccgtg gacggggcga gcgaccaggg cgggccctgc agggagaagg cgaggtagtt
     3601 gtagacggtg acgaagctcc ccatcacgag gaacgccgtg gcgtagagcc cgagaaggac
     3661 cgggtcgcgg gcgctcgagg cgagcccgct tcgcagggcc gacctcgtga gcggcctcgg
     3721 ccggaagcgg cgcgacggcg gaacggttcg cgcaaaccag agcgcgagca ggaggtcgac
     3781 gagcccgagg acgaggaggc cgccgcgcca gccgaggcgc gcggagagga gcccgacgag
     3841 gatccgcccg accatccccc cgacggcgtt cccggcgatg aagaggccca tcgcggcgcc
     3901 cgatcggcgc ggcgcgacct cctcggagag gtaggccatc gcgacggccg gcacgccggc
     3961 cagggcgagc ccctgcagtc cgcgcagcgc gacgagaagc gggaacgacg gcgcgaacgg
     4021 ggtcgcgaga ccgaggcagg ccgccagcag gatcgcgacc cggaggaccc cgacccgccc
     4081 cttcgcgtcg gagagcagcg cccaggcgag catcccgaac gcgagcgaga gcgtcgccga
     4141 ggagagggag aggctcgccg cggccggtga aaggccccat tccgacgcga ggagcggcag
     4201 gaccggctgc gtctcgtaga ggagcgagaa ggtgacgaag ccggcggcga agagctcgcg
     4261 ccccgtggct tcgggaggcc ccggggggcc ggctccgttc atcgggggcg gaggccgcct
     4321 ctcacgcgat cgcggacatc gtccgcgtga cgatctcccc cgcctcgtcg acgtcggagt
     4381 cgtcgacgtc gaggtgcgtc acgaagcgga ggagccaggg cgactcgtcg agcgcgagga
     4441 ctcccgcctc ggcgagccgt cgggcgacgt cggcggcctt cgtccccgtg agcctcgtcg
     4501 aggcgaagac catgttcgtc tccacacgcg cgaggtcgac ggagagcccg ggcgccttcg
     4561 cgatcgcctc cgcgagcgtc cggcagcggc ggtggtcctc cgcgaggcgc gccacgttct
     4621 cgcggagcgc gaagagcccc gcggccgcga ggatccccgc ctgcctcatc ccgccgccga
     4681 ggagcttccg ggcgcgccgg gcctttctca cgaagtcggc ggggccgacg aggaccgagc
     4741 ccacgggcgc gccgagcccc ttcgagaggc agacgctcac cgagtcgaac ggcgccgcga
     4801 tctcggagag cggcctgccg ctcgcgacgg cggcattgca gacgcgcgcc ccgtcgagat
     4861 gaaggacgag gccgtgccgg tcgcagaacg cccgcaccgc gaggaccccg cgcgagtcga
     4921 ggacccgccc gccgcagtgg ttgtgcgtgt tctcgagggc gacgaggcgc gtccgcgcgt
     4981 tgtgcgggtc ctcccccgtg tggacgaagt cctccatcgc ccccgggtcg agcatcccgt
     5041 cgtcggacgc gaagctccga gtctggagcg aggcgaggac tgccgcgccc gcctgctcgt
     5101 agaggaggac gtggctctgc tcgtggacga agacctcgtc gccgcgctcg gtccaggcga
     5161 ggagggccgc ctggttcgac atcgtcccgg aggggaggaa gagcccggcg gccttgccga
     5221 agagcgccgc ggcgtgctcc tcgagcgccc tcaccgtcgg gtcgtcgccg aagacgtcgt
     5281 cgccgacgac ggcttccgcc atcgcgcggc gcatcgcggc cgagggccgc gtcaccgtgt
     5341 cgctcctcag atcgatcgtc cgcatcgccg ggtccctccg ctccgggcga cggtacaccc
     5401 gtccgtcgcg gggggtggac cgcgggctca ggcgccttcg ggcctcgcgg ccatcgcgat
     5461 gacggagaag tgcgccccga gcgggtcgcg gaggacggcg aacgtcccga cgtgcgggat
     5521 ctcggtcggc ggcaccgccg ccccgccccc gagctcgccg gcccttcgag cggccgccgc
     5581 gcagtcgggg acgaggaagt agggcatcca gtgcggcggg acgtcgcccc accaatcccc
     5641 cttcatcgga aggaggcccc cgatcgcctg gtccccgcga tacgtctcgt ggtattcgac
     5701 ctcggacgtc tccgacggct tccaggtcca gccgaagagg ccgccgtaga acgccttcgc
     5761 accgtcgggg tccttcgtca cgagctccgt ccagcagagc gtgtggtcct cgccgacgag
     5821 ctgcgcgccg atgtgcccgc gcgcctccca gagcgcgaag acgacccctt ccgggtcggc
     5881 gacgaccgcc atccggccga ccccttccac gtcgaacggc ccgtggcaga cctttccgcc
     5941 cagctccgcc gccctcgcgg ccgaggcgtc gaccgaggcc gtggagacgt acgtcatgaa
     6001 gtgggacggc acgttctgcg cgagctcggg cccgctcagg gtccggaggg cggccacgtc
     6061 cttgccgcgc agcttcagca gcgtgtaggc gccgcctgcc gtcgggacgt cggcggcctc
     6121 ccacccgaag agcccggcgt agaacgcctt cgccttcgcg gagtcggggg tggcgagctc
     6181 gggccagcag aacgagccgg gcagcagctc cgtgcgctcg gacatggggg accctccttt
     6241 cgatgtgcgc ggatgctatg cgatcccgcg gcgtcccggc gacgcctcgc gagcgcgccg
     6301 gcacgggcgt cccgcttcgg cgtccctcgc gccccgtttc gtcccctgcc cctctcgttc
     6361 tacgcttgcc ggcggaggct cccatggccg aaggcaagct ccccctcgcc gcgttcgtcg
     6421 acgccgcccg cgggtccgtc cccgccgacc tcgtcttccg gaacgcccgg gtcgtcaacg
     6481 tcctgacggg cgagatccac gaggagaccg tcggcgtcgc gggcgaccgc gtcctcggat
     6541 tcgggccgta ccgcgcggcg cgccccgaga acgagatcga tctgaaaggc gcgttcctcg
     6601 ccccggcctt ctgggacgcg cacatccacg tcgagtcgac gatgacgacg ccccccgtct
     6661 tcgcccgcct cgcggccgcg cacggcacgg cggccgtcgt caccgacccg cacgagatcg
     6721 cgaacgtgcg cggcgccgag gggctcgagg cgttcctcgc ttcggccgac gggctcccgg
     6781 tggagctcca cgtcatgctc ccttcgtgcg tcccggcgac gcacctcgag acggccggcg
     6841 ccgacctcgg accggccgac ctcgcccgct tctacggccg gaagaacgtg ctgggcctgg
     6901 ccgagatgat gaacgtcccc ggcttcctct tcggcgaccc gaacgtccgc gcgaaggtgg
     6961 aggacgccgc ctcccggggg ctcgcgatcg acggccactc gccgctcctc tcgggcctcg
     7021 acctgaacgc ctacgtcggc gccggcatcc ggaccgacca cgagtgcctc ggccgcgagg
     7081 aggcggcgga gaaggtccgc cgcggcatgt cgatctggat ccgcgagggg accgccgcga
     7141 agaacctcga cgacctcctc ccgctcgtga acgcgacgaa ctccgagcgc ttcgccttcg
     7201 cgacggacga ccgccacccc tgggacctcc tgacccacgg ccacgtcgac caccacgtcc
     7261 gtcgcgccat cgcgctcggc ctcgacccgg tcctcgcgtt ccgcctcgcc tccctgtccg
     7321 ccgcgaggca ctacggattc cgcgacaggg gcgccgtcgc ccccggctac cgcgccgacc
     7381 tgatcacgtt cgagagcctc gacgacgtga ggcccgacct cgtcgtcctc ggcggtcggc
     7441 gcgtcgcgca gggcgggaag ctcctcgtcg acgtcccgtc gcgcggcatc cccgaggggc
     7501 cgtcgttccg ggtcgacggc ttcgacgcct cgcgcctccg cctccccgcc gagcccggcg
     7561 cccgggtgcg ggtcatcgag gtccaccccg gctccctcgc gaccggcgcc tcgaccgcgg
     7621 aggcgcacgt cgtcgacggc gccgccgccg ccgacccttc gcgcgacctc gcgaagctcg
     7681 ccgtcgtcga gaggcaccgc gggaccggaa acgtcggcgt ctccttcgcg cgcggattcg
     7741 gactcgcgcg gggggcgatc gcctcctccg tcgcgcacga ctcgcacaac gtcgtcgtcg
     7801 ccggctgcga cgacgcgtcg atggagacgg ccgtgcggcg cgcggccgag atcggcggcg
     7861 gcatcgtcgt cgcgctcggc gcgcaggtgc tcgccgaggt ggcgctcccc gtcgccggcc
     7921 tcatgtcgaa cgccccgatg gaggaggtcg accgcgccga gcggagggcg aacgaggcga
     7981 cccgcgccct cggcaccgcc gtcgaccacc ccttcatgac gctcgccttc ctcgccctcc
     8041 ccgtcatccc cgagctgaag ctgaccgaca aggggctcgt cgacgtgacc cggttcgagc
     8101 atgtctccgt gttcgcgtag gccgacccgg acccggagcc cggcgcgcga tcgctnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccgca tctccggacc ccgcgagtct
     8281 cgagcgcggc ggaggagatg cctacggagg cctcgcgtga cggccgtcgg cgtcctcgcg
     8341 ttcctctcgg tcaagctcgt cgcctacgcc gcgtggtgcc ggctggggct cgggcttctc
     8401 gcgccggagc tccccgcggc ggcgctgcga cgagcgggat tcctcgcctt cgtccgcctc
     8461 gcgatcggcc tcctcctcgg gtggctcctc ctcctcggcc tcgccaccct cgtcccggcg
     8521 ccgaaccgtc tcggcgtccc gccgctcgcc ctcctcgccg gctctgcgct gctccgctgg
     8581 ctggagtggt cgttcgtcgg cgcgctcgcc gccgggcgcg cgcgctctcc ccgcgccgtc
     8641 ctcctcggcc ggagcgggaa ggagcacctc tggcgcgtcg gcggcgtcgc cctctcgttc
     8701 gcgaccgacc tcgccggcct gctcggcgcc ggcgccctgg gagtcatccc gtgttgagag
     8761 aagtcccgcc cgctgcgaag gaggcccgca tgaagaggat ccgtcactcg atcaccatcc
     8821 gggcatcgcg cgacgccgtc tgggccgcga tgctcgggcc cgagacctac cgggtctgga
     8881 cgacgccgtt ctgcgagggc tcgtactacg agggctcctg gaacacgggg gaccggatcc
     8941 gcttcctcgg accgggcggc gaagggggaa tgacctccgt catcgccgag agccgcccgc
     9001 cggagttcgt ctcgatcaag cacctcggga tcgtcaagga cggcgtcgag gacacgacga
     9061 gccccgcggc cacggcctgg gccccctcgt tcgagagcta cacgttcgtc gacgtcggcg
     9121 gcgcgacgga ggtgaccgcc gagctggaga cgccccccga cttcgaggcg ttcatggacg
     9181 acgcgtggcc gaaggcgctc gcgaagctga aggagatctg cgaggagtcc gcctcccgct
     9241 gagccgtcgg ccgggcggcc tccgtcccct cgaagcgatt cctcccacgc gcggggctat
     9301 cctcgcgcgg aggcaggtcc cgcgcatgaa cgtcctcgac tccatcggca gcacgtccct
     9361 cgtccgcctc cgcaaggtcg tccccgcggg ctccgcggac ctcctcgtca agctcgaagg
     9421 ggagaacccg accgggagca tgaaggaccg gatggcgctc gccgtgatct cgcgcgccga
     9481 ggccgacggc cggctccggc ccggcggcac cgtcgtcgag tacaccgggg ggagcaccgg
     9541 gacctcgctc gcgttcgtcg ccgccgcgaa gggctaccgg atccggatcg tcacgtccga
     9601 cgccttcagc cgcgagaagc gcgatcacat ggccgcgctc ggcgccgagc tgacgctcgt
     9661 cccgagcgag gggagcctga cgacgaagaa gctgatcctc gacatgatcg cggccgcccg
     9721 cgagatcgcc cgggagccgg ggaccttctt cacgaaccag ctcgagaacg ccgacgcgat
     9781 cgccgggtac gtcccgctcg ccgaggagct cctcgagcag acgggcggcg agatcgacgc
     9841 cttcgcccac tccgtcggga cggcggcctc gctgcgcggc atcgcggccg tcctgaaggg
     9901 ccggcgcccc gggctgcgaa tcgtcgccgt cgagcccgcg gagtcgtccg tcctcctcgg
     9961 gagcccggcc ggcccccaca agatcgaggg ggtcggcgtc ggctacgtgc cgcccctctg
    10021 ggagccctcg ctcgtcgacg aggtcctcgc cgtcgcgaca gaggaggcga aggagatgac
    10081 gcggcgcctc gcgcgcgagg aggcgctctt cgccggcacc tccaccggcg cgaacgtcgt
    10141 cgcggcgctc cgcgtcgccg cgcgcctcgg ggcgggagcg acggtcgcca ccctcgcgtg
    10201 cgacaccggg ctcaagtacc tgagcaccga cgtctaccgt cgctcctgag gcggggccga
    10261 cgccggctcg cggggactcc cggcctccca cgcccgccgc cgagccgcta cgatcggcgt
    10321 gtgattacct tcttcacgcc gctccacgaa ggccacgccc cgcgctacga gtacttccgc
    10381 ggcgagcggg tcccctgctt cgagtccccg gtccgcgccc gctacgtcca cgacgcgctc
    10441 gtcgccgccg gacacgagct ccgttcgccg acggtcgacg gcgctcccct cctcgtccgc
    10501 gtccactcgc cgcgctacct cgcgttcctc gagtccgcct ggagccagtg gctcgcgcgc
    10561 gacccggcga acgccgccgt ccagccgttc ccgtccgtct ggccggtccg gacgctccgg
    10621 agcgacgtcg agccggacga cttcgtcgcg cgcctcggcc tctactcgat ggacaacggg
    10681 acgccgctcg cggaggggac ctgggccgcc gtgaaggccg cggccgacgc cgccgcgagc
    10741 gcggccgctg tcgtggcctc cggcgtccgg tgcgccttct cggcgacgcg cccccccggg
    10801 caccacgccg gggccgactt catggggggc tactgcttcc tgaacaacgc cgcggtcgcc
    10861 gccgaggcgc tcctcgggag cggctcgaag cgcgtcgcgc tcctcgacgt cgactaccac
    10921 cacggcaacg gcacgcagag cctcttctac ggccggtccg acgtcttctt cgcgagcctc
    10981 cacgccgacc cgcgcttcga gtacccgttc tacctcggtc acgccgacga gacgggcgag
    11041 ggcgccgggg agggcttcaa cctgaacctc ccgctcccgg ccggcacgcg cgggccggag
    11101 tggctcgccg ccctggagac ggcctgcgcc cgggcggcgg cggccggcgc ggacgccctc
    11161 gtcgtctcgc tcggcctcga cacgttcgcc ggagacccgc tgacgaggtt cgccctcgaa
    11221 cgggatgact tccgtcgcct cggagagcgc ctcgcgcgga ccggcctccc gaccgtcctc
    11281 gtcctcgaag gaggctatgc ggccgccgag ctcggcgcga acgcggcgag cgtcctcgag
    11341 ggcttcgagg gcgcctgacg gacggcggcg gcgcgggcgt tccggccggt gcacggctgc
    11401 cggacgccgc atcgagtcgg cgggacctcc ggcccgtgga ccccggcgcg tccggggcgc
    11461 tacgctcccg ccgtgcactc cgacctcgcc tcgacgctcg cggactcccg ctccgcgacg
    11521 acccggtggc tcgcgctcgt cgagcctctc tcctccaccc aggcgaactg gcgccccgct
    11581 ccggaccgct ggtccatcgc gcaggtgctc gaccacctcg ccgcgacgac cggccccctg
    11641 gccccggtcc tcctcgaggc cggcgcgaag gcgcgctccg cgggccgctt ctccgacggc
    11701 ccgttccggt acgggccgct cgcccgctgg ctcctccggt cgctggcccc tgccggcgcg
    11761 cggcccctcc ccgcgccgaa gctctatcgg ccctcggcct cggcgctcga cctcgccgag
    11821 gtgacccggc gtttcctcga ggcccagcgc gcgttcgaga gcgcggcgga gtcggccgag
    11881 gggctcgacc tcgcccgggt ccgcgttccc tctccggccc ttcgcctctt ccggcttccg
    11941 ctcggcgtct ggtgcctctc caccgcggcg cacaccctcc gccacctcgc ccaggccgag
    12001 cgggtccgcg ccgactcccg ctttccggaa gatcgcccgg atcggggtca ggcgtgaggt
    12061 cgagtcgtgc tggaaacacg acacgatttc acgcctgacc ccggccgtcg ggggcatccg
    12121 acttaggatc gcggtccatg agcagcgttc gcccggtctc cgggacctac agtcttcatt
    12181 tcgctcccta catcgcgctc gtgaccgagc ccgacgtcct cgccgtcctc gagacacagg
    12241 gggcggcgtt cgagccggca ctgcgccgcc tcccggccgc ggcggcgcgc gcccgctacg
    12301 ccgaggggaa gtggagcgtc gcccaggtcg tcgggcacgt cgtcgactgt gagcgggtgt
    12361 tcggctaccg ggcgctcgcg accgcccgcg gcgagaaggc ggccctgccg agcttcgacg
    12421 aggacgccta cgccgccgcg gccgggcacg acgactgccc cctggtcgag ctgctcgacg
    12481 agctcgcggc gcttcggcga agccacgtcc tgatgttccg ccacctcgac ggcgccgcct
    12541 gggaccgcct cggcctcgtg ggggggcacc cgaacgccgc ccgcgcctgg gcgttcgtca
    12601 tggcgggaca cctccgccac cacgcgaacg tcctccgaga gcgctacggc gtcgcggtcg
    12661 aggcgtgagc ggagcgtcta accccgggga gctcgcgcat cggcgctccg cgatgaccgt
    12721 cgtcctcgag agccgccccg cgaccgcacc cgcccggacc cggaggccgt ggaacgcatg
    12781 aagcacctgc cgaacctctt cgagaacaac cgcgcctggg ccgacggcct ccgccgggac
    12841 gacccggagt tcttcacgcg cctctgccgc cagcaggcgc ctccctactt ctggatcggc
    12901 tgctccgaca gccgcgtccc ggccaacgag atcgtcgggc tcctgccggg cgagctcttc
    12961 gtccaccgga acgtcgcgaa cctcgtcgtc cagagcgacc tgaacgccct ctccgcgctt
    13021 cagttcgccg tcgacgtcct gcgcgtgaag cacgtcgtcg tctgcgggca ctacggctgc
    13081 ggcggcgtcc tcgcggcgct ccggggagag cggcacggcc tcgtcgacaa ctggctccgc
    13141 cacgtcgccg acgtccggga gaagcacgac gcgcagctcg gcaggctggg cccggagcgc
    13201 gcggtgcgcg accggctctg cgagctgaac gtcgtcgagc aggtccggaa cgtcgcctcg
    13261 acgaccgtcg tccgcgacgc ctgggcgcgc ggccaggagc tctcgctcca cggctgggtc
    13321 tacgccctcg aggacggcct cctgcgcgac ctcggcgtct cggcgaaggg ggaggcggag
    13381 ctggccgagg cgtacgcgcg cgccgtggcg gggcggcccg acggcgtcgc tccgccggcc
    13441 ggggagcggg gcccctgagg gtccgtcagt agtactcggc cagggcgagc gtcctctccg
    13501 ttccgtcggc gagggtgaag cggactttcg ttcccgccgg cgcctcggac cagcggacct
    13561 ccgtccgcca cgactcgggc cccgccggct ctccgtcgag cgccgtgaca cgctcgcctt
    13621 ccttccaccc tgtcgccgcg gcggggcttc ccggcgcgac gtgctcgacg acgagagccc
    13681 cgtcggccca gtccaggtcg agaccggtct tgtccttcgg gaacggcgcg gcgagacccg
    13741 gtcccggctc gagccagagt gcggagcgcg cgtagtcgaa gaggacccgg aagcgggcga
    13801 ggatttccgc gcccaggttc ccctcctgcc gcttcgtgtc gacggcgccg ccgacgtccg
    13861 tcgtctggaa ggtcgccggc acgttccgca ggtcgtagcc ggcgacggag accgagcgga
    13921 gcgtcgcgag cttcgagagt gtggcgccgc cgacgccgcc gcctcgcctc tcggagaccg
    13981 ggcgcccttc gaggaggcga cgcgcgtcgg cgtagtgccg gaagaccgag agcgcgcccc
    14041 cctgccccgt gccgagcccg acgacgaccg gcgcatcccc ctcgatcgag atccggaggc
    14101 tcttgtgccc gtcctcggcc gggaggaggt cgaccttccg gccggggccc gcgtatcgga
    14161 acgacgccgc gtcgtggagg cgcaggcgcg acccggggta gtccaggtcg acgacgaggg
    14221 cgtggaagag ctccttgccg aggatga
//
LOCUS       DAOTEZ010000058        14095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_2988_length_14095_cov_5.972293, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000058 DAOTEZ010000000
VERSION     DAOTEZ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14095
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2988_length_14095_cov_5.972293"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="P5164_04705"
     CDS             complement(<1..694)
                     /locus_tag="P5164_04705"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRY43228.1"
                     /translation="MLRLVVRYRHETLVFPFPSAEVTLGSSSKADLVVPFPGVSRLHA
                     RISPEAYGIRIVDEGSKNGVIRNGKRTLRAALREGDEVLLGDATLALQEAGEAEDLAL
                     ELGPSQRDQAPPTGSTASGPLDAPASALRLVRELEGDGRETPARRRALLRKARAILGA
                     EGLVALDSLAASEPAFLDLDGPAPEKALLETLRAVIGKRTSSRAPLSLLLGNGRSAAV
                     APSIIRSTSTTWW"
     gene            complement(736..3453)
                     /locus_tag="P5164_04710"
     CDS             complement(736..3453)
                     /locus_tag="P5164_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRY43229.1"
                     /translation="MDSRSTDTSGLEREPLRPLPPPASGDVLAGRWELRRLLGQGGSG
                     LVFEAWDRELKGAVALKLLRPDRLSPASLARLRREVKVAHAVASPRLVRVYDLGEDHG
                     RPFVTMELVAGAPLRDRTTGPLPVGDVVRIGIEVLEGLAALHAAGIVHRDVKPGNVLL
                     GPDGGARIVDFGLARSLDRDETRLTETETALGTADYMAPEQALGHDVDARSDLYAFGV
                     LLFELLTGDLPWRAESSFGSVLARLRAKAPDVRKLRPETPRWLAETVGRLLERKPENR
                     FPSAEETLRVLRRRSVGLRAILARRRRPLLAVAAALALALLGAAGWRLAARPSLATVS
                     ADGAFGARGLDRSGRVLWRRPDLDPARCAVVGRFRKDGPEEVAAILENEVYDKEPERI
                     YSLSFLEAATGRVLRTERLTPDLEGFPPAERRFAVTTMKAIDTDGDGLDEVFVSYFHS
                     WAPSFVLMWNPAERRSLMAFTGGGHHRLVDAADVDGDGRKEMLLLGINSPYGWYRAFA
                     IARLWTDDPEGAPSRTVPFGAFSADQAGGADASNLIDYVLLPRGVFSEDSRELRAVDG
                     TSFAIEYGNGRTVRIDLGGCGLLSPADGLDCRTLRRSRMSAYANLREGLRRGRAAAPD
                     EAVESFDAAERDARSARDPILLELVRRWRGMTLLLHGDAREGAAVLAGLVRGSENALE
                     IADDAGRELHKAGRIPEAIAWYSRALERAGPEHTGRSVTYPREGLLLGLAELGRWSEA
                     SRWIDRWAAAANMRVVARVPWYRTFLAWRQGIAFDPPPVEAGTYDLLHLWSFEMLLAK
                     NAEPKALLERARSEFVEAVRARGPLLSVQAELLERLGRRDEARDVAREAWRETEAAVR
                     TEVWTRAHLDLVVERVVRLAGGDEARHARALLETVRAEAKRPDPGTRTR"
     gene            complement(3489..4109)
                     /locus_tag="P5164_04715"
     CDS             complement(3489..4109)
                     /locus_tag="P5164_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HRY43230.1"
                     /translation="MALPRRPSARLAALLGAALAVAAPAARAADPPRSGVISRPKGFS
                     TIRLVAPPGLFVFLGDVPVGTTDVLRQGLWLEDLPAGRYVLRVEKPGFEPKVVTVVVG
                     EGDTKEVRVLALRPRPTPTPAPPETAASPAPTPPAASPAPVRAVVLERKPVPGGTLVT
                     GIASSAVDALPLLEELKTECRCAPALESMTKRKDGLYEFRVKLPGT"
     gene            4213..4485
                     /locus_tag="P5164_04720"
     CDS             4213..4485
                     /locus_tag="P5164_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008294069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HRY43231.1"
                     /translation="MHWKTFATVFFTVLVAEMGDKTQLATLLFSADRQTSKWVVFAGA
                     ALALTAAAGIGVLVGSQLERFLSPKLLKTIAGVGFLAIGAWTLLSK"
     gene            4614..>5689
                     /locus_tag="P5164_04725"
     CDS             4614..>5689
                     /locus_tag="P5164_04725"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HRY43232.1"
                     /translation="MRKPAALLVLATTLALAAPDAPACTNILVTKGASADGSTMITYA
                     ADSHELYGELYFTPAGVHTFGAMREIVEWDTGKTLGRIPQAPVTYQVVGNINEHQVSI
                     GETTFGGRKELEGPSGIIDYGSLMWIGLERSKTAREAIEVMTKLVDEFGYASSGESIS
                     IADPNEAWVLEIIGKGEKQKGAVWVAVKVPDGTISAHANQARIRQFPRNDPKNVLFSK
                     DVVSFARDKGWFTGKDEEFSFADAYAPLRASTLRSCDGRVWSVFRRAAPSLNLSSDWI
                     LAVPGAKPMPWAIKPDRKLTTKDVMELMRDHFEGTELDMTKDIGAGPFVLPYRWRPMG
                     FKVDGVDYVHERAISTQQTGYSFVA"
     assembly_gap    5690..5789
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5790..6406
                     /locus_tag="P5164_04730"
     CDS             <5790..6406
                     /locus_tag="P5164_04730"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HRY43233.1"
                     /translation="YVHERAISTQQTGYSFVAQLRSFLPNPVGGVLWFGVDDTYSTVY
                     TPMYCGIREVPKSFAVGTADFSAFSWDSAFWVFNFVTNWAYSRYSDMIVDVRAAQDEL
                     EGRFLAEQPEIEKEAVRLHAASPAAARDYLTKYSVGAGDLVFARWKKLGDHLLWKYMD
                     GNVRDTQGGVTHPRYREEWYRAIAKDGGDRLKARKLPEEPKRPE"
     gene            6483..8729
                     /locus_tag="P5164_04735"
     CDS             6483..8729
                     /locus_tag="P5164_04735"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HRY43234.1"
                     /translation="MKARGSLLLLAGLAIVAGPALCLSEGADGLRGAEIRISRATGPI
                     SVDGDLGDAGWLGAARVETFYETNPGDNLPPKVRTVGRLAYDDRFLYAALECEDPEPG
                     KIRAPFSQRDVLGSGTDYAGIILDTRNDGRTAILFLANPRGIPYDAVTDDGNEDSSPD
                     FHWEAAGKITATGWNLEIRIPFSSLRYDRADPQTWGILLYRNRPRDFRYQMFSARLPR
                     GRNCFICSENVLTGLEGLPPGGHLVVAPYTTASQETLPRADGTGSVAKPVEPDGGLDV
                     KWTPSAGVALDGTFNPDFSQIESDTARIGVNERFALFYPEKRPFFLEGVDLLSTPVQA
                     VYTRSVTSPRWGLRGTGKLGGTSWTALVAEDRGGGSVILPGPNGSDLAEQDFRSLDLV
                     GRVRRDVGRSFASFLVTAKEMPGSASNRVLGPDFRLNLSETDLVTGQLLWSWSETPDR
                     PDLAAEWDGRSLSGHGADVWWEHTTETWDWGLEYKDFSDGFRADLGFVPQVGFREGYA
                     GAGRTFRPKGFFSRVRTFFFADYLVDREGELLSQVVAPGVGFDTKFNGFARFELRSEK
                     VRAGTRVLPRKRVVYSLSAVPSRFFAGIAIDGAIGEEIDFEGARTGKGGWLAASAVFR
                     PGDHLRLDLSGEVRWLDVDLPDGRSPRLFTAHVERLKATWTLTARAWVRVIGQYVGAW
                     QAPELYPTEVEAESGTFSASALLGYRLNWQSVVFLGYGDARTLSEDGGLPPESRQLFL
                     KVSYAFQR"
     gene            8816..9112
                     /locus_tag="P5164_04740"
     CDS             8816..9112
                     /locus_tag="P5164_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43235.1"
                     /translation="MLARERLAKCPACRGTGLGGPCARCSSLGRIHFQGNEEQWILCG
                     YCRGTGTVDCPRCRREGRIHFFSFQCPRCGFVGPESEGHLDELYADLCPKCGSY"
     gene            complement(9150..9866)
                     /locus_tag="P5164_04745"
     CDS             complement(9150..9866)
                     /locus_tag="P5164_04745"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HRY43236.1"
                     /translation="MSFVSSEEKTLAFVAAHLGASPKGPPRSRERRRPVVTVSRETGA
                     GGNTFGEALCAWLEEHQPKGRGPWALVDRELVDKILEDDALPDRLASWSPEDHLAGVS
                     FVIEELLGLHRATWKPMQETTETILRLGEMGNVVLVGRGANVILGHLPQAFHVRLVAS
                     LESRLENVAEARGLSKKAARAWVEAEDKARRRFIRRYFQVDVADPLLYNVVVNVDRVP
                     IDEAAGIVGEAVLARAGRGG"
     gene            10095..12242
                     /gene="fadB"
                     /locus_tag="P5164_04750"
     CDS             10095..12242
                     /gene="fadB"
                     /locus_tag="P5164_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011104595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HRY43237.1"
                     /translation="MKYEGKAIQTVPLDEGFVELRFDLQGDSVNKFNSLTLGELKEVV
                     AGLTAAPPKGLLITSGKDVFIVGADVTEFLTHFKRSEEELAGWLLETDATFSAIEDLP
                     CPSVVAINGFCLGGGFELALSAHFRVASTAAKVGVPETKLGIFPGWGGTVRLSRMCGA
                     DNAIEWIATGEQYPAAEGLKIGAVDAVCEPEKLREVALRMLADAADGKLDWKRRVEEK
                     KAPLPLNKVEAGMVFEGGKAFVGGKAGPNYPSPVAAIEAMQKGATLGRDEALKIEAAT
                     FARIAKTPTAHGLVSVFLGDQLVKKLAKKASGGAKPVQRAAVLGAGIMGGGIAYQSAS
                     KGVPILMKDIAPKQLELGMGEAAKLLEKQVERGKMTAGKMAQVLAGITPTLSYGDFGA
                     VDIVVEAVVENEKVKKSVLADLEKSVKPGTILASNTSTISITRLAEALAAPENFCGMH
                     FFNPVPRMPLVEVIRGAKSSPEAIATTVAYATAMGKTPIVVGDCAGFLVNRVLFPYFT
                     GFELLLADGVDYKRVDKVMEKWGWPMGPALLLDVVGIDTGVHADKVMAAAFPDRMSHA
                     GESAIEKMVAAQRYGQKNGKGFYVWEPQKKGPPKKGVDPFTEEAMKAHAKASPEVADA
                     EIVERMMLPMLLECSRCLEDGIVATPTEVDLALVYGLGFPPFRGGIFRWADATGTKAL
                     LAAAEKHAPLGALYAPTEQLKGLAASGKGFHGE"
     gene            12247..13410
                     /gene="fadA"
                     /locus_tag="P5164_04755"
     CDS             12247..13410
                     /gene="fadA"
                     /locus_tag="P5164_04755"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HRY43238.1"
                     /translation="MKDVVIVDAVRTPMGRSKGGMFRNVRAEKLSAEVINALLARNPE
                     VNPADVDDVIWGCVQQTLEQAYNVARFAQLMTPIPKQVPAQTINRLCGSSMTALHSAA
                     MAIMAGYGDLFVVGGVEHMGHVPMTHGVDFAPQNAKRNAKAAGAMGLTAEYLGRLHGI
                     TREQQDAFAYRSHMRAWQATQSGAFKPEIVPLEGHDEDGALKLFDFDEVIRPETTLEG
                     LAKLKPAFDPKSGTITAGNSSGISDGAAALLVMSAEKAKELGKHPMARIRSMSAAGCD
                     PSIMGYGPVPAVKKAFARIGMKSGDVDLWELNEAFAAQSLPVLKDLGLLDVMEEKVNV
                     NGGAIALGHPLGCSGARITATLLHAMKAKGARVGVATMCIGFGQGVATVFERV"
     gene            13484..>14095
                     /locus_tag="P5164_04760"
     CDS             13484..>14095
                     /locus_tag="P5164_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HRY43239.1"
                     /translation="MRLVTRGDLDGLTCSVLITSVEKVDAIELVHPQELTDGRFEVRD
                     GDVLANLPFHPGCSIWFDHHELVDANPRPEAAFQGRYGLAPSTARLVHDYYCERKGWR
                     LFERYGELVAGTDRLDSANLTRRDVLDPQRVILLGFTIDSRTGLGGFRDYFLFLGMAL
                     RSMPLDEVLRSPQVTERVAKMKEADEEFRRALLASSHVDGNVVV"
BASE COUNT         2083 a         4877 c         5118 g         1917 t
ORIGIN      
        1 tccaccaggt tgtcgatgta gagcggatga tagaaggtgc gaccgccgcc gaacgtccgt
       61 tgccgagcag gagagaaagc ggagcccggg acgaggtgcg cttgccgata acggcgcgca
      121 gggtttcgag gagggccttc tcgggcgccg ggccgtccaa gtcgaggaag gccggctccg
      181 aagcggcgag cgagtcgagc gcgacgagac cttcggcgcc caggatcgct cgggccttgc
      241 gcagcagcgc acgccgccgg gccggcgtct cgcgaccgtc tccttccagc tcccggacga
      301 ggcgcagggc cgaggcggga gcgtcgagcg gtccgcttgc cgtcgagccg gtcgggggag
      361 cctggtcgcg ctgcgacggg ccgagctcca gggcgagatc ctccgcctcg ccggcctcct
      421 ggagtgccag cgtggcgtcg ccgagaagga cctcgtctcc ctcacgcaga gcggcgcgaa
      481 gggttcgctt accgttccga atgacgccat tcttcgagcc ttcatccacg atgcgaatgc
      541 cgtaggcctc cggcgagatc cgggcgtgga gacgggagac gccgggaaat ggcacgacga
      601 ggtcggcctt cgaagacgag ccgagggtga cctccgcaga gggaaacgga aagacgaggg
      661 tctcgtggcg gtagcggacg acgagcctca gcatcgagca cctcagatcg gctgcgagca
      721 aacgccgtgc cccgctcatc tcgtccgcgt ccccgggtcc ggccgcttcg cctcggcgcg
      781 gaccgtctcg aggagggcgc gggcgtggcg cgcctcgtcg ccgccggcga ggcggacgac
      841 gcgctcgacg acgaggtcga gatgggcgcg ggtccagacc tccgtccgga cggccgcctc
      901 cgtctcgcgc caggcttccc gcgcgacatc gcgggcctcg tcgcggcgac cgaggcgctc
      961 gaggagctcg gcctggacgg agaggagcgg cccgcgggcg cggacggcct cgacgaactc
     1021 ggaccgggcc cgttcgagga gggccttcgg ctcggcgttc ttcgcgagga gcatctcgaa
     1081 cgaccagagg tggaggaggt cgtacgtgcc ggcttccacc ggcggcggat cgaaggcgat
     1141 gccctgtcgc caggcgagga aggtccgata ccacggcacg cgggcgacga cgcgcatgtt
     1201 ggccgcagcg gcccagcggt cgatccagcg ggacgcctcg ctccagcgac cgagttcggc
     1261 gaggccgagg aggagccctt cgcgcgggta ggtcacggag cgcccggtgt gctccgggcc
     1321 ggcccgctcg agggcgcggg agtaccaggc gatcgcttcc gggatgcggc ccgccttgtg
     1381 gagctcgcgg cccgcatcgt cggcgatctc gagggcgttc tccgagccgc ggacgaggcc
     1441 ggcgaggacg gcggcgccct cccgcgcgtc cccgtgaagc agcagcgtca tcccgcgcca
     1501 gcggcggacg agctcgagga ggatcgggtc gcgggcggag cgcgcgtcgc gctcggcggc
     1561 gtcgaagctc tccacggcct cgtcgggggc ggctgcccgg ccgcgccgga ggccttcgcg
     1621 caggttcgcg taggccgaca tccggctccg ccggagcgtc cggcagtcga ggccgtcggc
     1681 cggggagaga aggccgcagc cgccgaggtc gatgcggacg gtccggccgt tcccgtactc
     1741 gatcgcgaag gacgtgccgt cgacggcgcg cagctcgcgg gagtcctcgc tgaagacgcc
     1801 ccgcggcagg aggacgtagt cgatcaggtt cgaggcgtcg gcgccgcccg cctggtcggc
     1861 cgagaaagcg ccgaacggga cggttcggga cggcgcgccc tcggggtcgt ccgtccagag
     1921 ccgcgcgatg gcgaaggcgc ggtaccagcc gtaagggctg ttgatgccga ggaggagcat
     1981 ctccttccgc ccgtcgccgt cgacgtcggc cgcgtcgacg aggcgatggt gcccgccgcc
     2041 ggtgaacgcc atgagcgagc gcctttcggc ggggttccac atcaggacga aggacggcgc
     2101 ccaggagtgg aagtagctga cgaagacctc gtcgaggccg tcgccgtccg tgtcgatcgc
     2161 cttcatcgtc gtgacggcga agcgccgctc ggccggggga aagccctcga ggtcgggcgt
     2221 gagcctctcg gtccggagga cccgcccggt cgcggcctcg aggaaggaga gcgagtagat
     2281 ccgctcgggc tccttgtcgt agacctcgtt ctcgaggatc gccgcgacct cctcgggtcc
     2341 gtccttccgg aatcggccca ccactgcgca gcgcgcgggg tcgaggtcgg gccggcgcca
     2401 gaggacgcgg cccgagcggt cgaggccgcg cgcgccgaag gccccgtcgg cggagaccgt
     2461 ggcgagcgac ggcctcgcgg cgagcctcca cccggcggcg ccgaggaggg cgagcgcgag
     2521 cgcggcggcg acggcaagga gcggacgccg ccgacgggcc aggatcgccc ggagcccgac
     2581 cgacctgcgg cgcaggacgc ggagcgtctc ctcggcgctc gggaaccggt tctccggctt
     2641 cctctcgagg aggcggccga cggtctccgc gagccatcgc ggcgtctcag ggcggagctt
     2701 ccttacgtcg ggcgccttcg cgcgaagccg cgcgaggacg gagccgaacg acgactcggc
     2761 cctccacggg aggtcgcccg tgaggagctc gaagaggagg acgccgaagg cgtagaggtc
     2821 cgatcgcgcg tcgacgtcgt gcccgagggc ctgctccgga gccatgtagt cggccgtgcc
     2881 gagggccgtc tcggtctcgg tgaggcgcgt ctcgtcgcgg tcgaggctcc gggcgaggcc
     2941 gaagtcgacg atccgcgccc cgccgtcggg gccgaggagg acgttgccgg gcttgacgtc
     3001 gcggtggacg atcccggccg cgtggagggc ggcgagccct tccaggacct cgattccgat
     3061 ccggacgacg tcgccgaccg gaagcggtcc cgtcgtccgg tcccgaaggg gagcgccggc
     3121 gacgagctcc atcgtgacga acggacgacc gtggtcctcg ccgaggtcgt agacgcggac
     3181 gaggcgcggg ctcgcgaccg cgtgcgcgac cttcacctcg cgccgcaggc gggcgaggga
     3241 ggcgggggag agccggtcgg gccgcaggag cttgagggcg acggcgcctt tcagctcgcg
     3301 gtcccacgcc tcgaagacga ggccggagcc cccctggccg aggaggcggc gcagctccca
     3361 cctccccgcg aggacgtcgc cggacgcggg aggagggagc ggccgaagcg gctccctctc
     3421 gaggcccgag gtgtcggtac ttcgggaatc catgccttcg agtccgcccc gtcggcgatg
     3481 tccgtagtct acgttcccgg cagcttcacg cgaaactcgt acagcccgtc cttccgtttc
     3541 gtcatcgact cgagagcggg cgcgcagcgg cactccgtct tcagctcctc gaggaggggg
     3601 agggcgtcca ccgcgctcga ggcgattccc gtcacgagcg tgccgccggg gaccggcttc
     3661 ctctcgagga cgacggcgcg gacgggcgcc gggctcgccg cgggtggggt cggggccggg
     3721 gacgccgccg tctccggcgg agccggcgta ggagtcggcc tcgggcggag cgcgaggacg
     3781 cgcacctcct tcgtgtcgcc ctcgccgacg acgaccgtga cgaccttcgg ctcgaagccg
     3841 ggcttctcga cgcgcaggac gtagcggccc gccgggaggt cctccagcca gagcccctgg
     3901 cggaggacgt ccgtcgtgcc gaccgggacg tctccgagga agacgaagag gccgggcggg
     3961 gcgacgaggc ggatcgtgga gaagcccttc gggcgggaga tgacgcccga cctcggcggg
     4021 tccgcggcgc gcgccgcggg agcggcgacg gccagcgccg cgccgaggag cgcggcgagc
     4081 cgggccgaag ggcgacgggg aagggccacg gagcgatcgt agcggccgga gaggcggggc
     4141 gcgagcggct ccggtatcct cccgcgttcc gggcgcgccg ccctgcgggg cccccggaac
     4201 ggaggcggtc cgatgcactg gaaaacgttc gcgacggtgt tcttcacggt cctcgtcgcg
     4261 gagatgggcg acaagacgca gctggcgacg ctcctcttct cggccgaccg ccagacgagc
     4321 aagtgggtcg tcttcgcggg cgccgcgctc gccctgacgg cggcggccgg gatcggcgtc
     4381 ctcgtcggct cgcagctcga gcggttcctc tcgccgaagc tcctgaagac gatcgcgggc
     4441 gtcggcttcc tcgcgatcgg ggcctggacc ctcctctcga agtaggaccc gcggggtccg
     4501 gtgtcccgac acgggccggg cgcggccgtc ccgcctccgg tacgatcctc gcggggcggc
     4561 tccccgcggc gatcgcgggc agggcggccc cgtcaaggag ccgcgaaagc cgcatgagaa
     4621 agcccgccgc cctcctcgtc ctcgcgacga ccctcgccct cgccgcgccc gacgcccccg
     4681 cctgcacgaa catcctcgtg acgaaggggg cgtcggccga cggctcgacg atgatcacgt
     4741 acgccgccga cagccacgag ctctacggcg agctctactt cacgccggcc ggggtccaca
     4801 cgttcggcgc gatgcgcgag atcgtcgagt gggacaccgg caagacgctc ggccggatcc
     4861 cgcaggcgcc ggtgacctac caggtcgtcg gcaacatcaa cgagcaccag gtctcgatcg
     4921 gcgagacgac gttcggcggg cggaaggagc tcgaggggcc gtccgggatc atcgactacg
     4981 gctcgctcat gtggatcggc ctcgagcggt cgaagacggc ccgcgaagcg atcgaggtga
     5041 tgacgaagct cgtcgacgag ttcggctacg cctcgtcggg cgagtcgatc tcgatcgccg
     5101 acccgaacga ggcgtgggtc ctcgagatca tcgggaaggg ggagaagcag aagggggccg
     5161 tctgggtcgc cgtgaaggtg ccggacggga cgatctcggc ccacgcgaac caggcccgga
     5221 tccggcagtt cccgcggaac gacccgaaga acgtcctctt ctcgaaggac gtcgtctcct
     5281 tcgcgcgcga caagggctgg ttcacgggga aggacgagga gttctcgttc gccgacgcgt
     5341 acgccccgct ccgcgcgagc acgctccgga gctgcgacgg gcgggtctgg agcgtcttcc
     5401 gccgcgcggc gccgtcgctg aacctctcgt cggactggat cctcgccgtc ccgggcgcca
     5461 agccgatgcc ctgggcgatc aagccggacc ggaagctgac gacgaaggac gtcatggagc
     5521 tgatgcggga ccacttcgag gggaccgagc tcgacatgac gaaggacatc ggcgccgggc
     5581 cgttcgtcct cccgtaccgc tggcggccga tgggcttcaa ggtcgacggt gtcgactacg
     5641 tccacgagcg ggcgatctcc acgcagcaga ccggctactc gttcgtcgcn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna ctacgtccac gagcgggcga tctccacgca
     5821 gcagaccggc tactcgttcg tcgcccagct ccggtcgttc cttccgaacc cggtcggcgg
     5881 cgtcctctgg ttcggcgtcg acgacaccta ctccacggtc tacacgccga tgtactgcgg
     5941 catccgcgag gtgccgaaga gctttgccgt cgggacggcc gacttctcgg cgttctcgtg
     6001 ggactcggcc ttctgggtct tcaacttcgt cacgaactgg gcctactcgc gctacagcga
     6061 catgatcgtc gacgtccgcg cggcgcagga cgagctcgag gggcgcttcc tcgccgagca
     6121 gcccgagatc gagaaggagg ccgtgcgcct ccacgccgcc tctccggcgg cggcccgcga
     6181 ctacctgacg aagtactcgg tcggggcggg cgacctggtc ttcgcccgct ggaagaagct
     6241 cggcgaccac ctcctctgga agtacatgga cggcaacgtc cgcgacacgc aggggggcgt
     6301 cacccacccg aggtaccgcg aggagtggta ccgggcgatc gcgaaggacg ggggcgaccg
     6361 gctcaaggcc cggaagctcc ccgaggagcc gaagcggccc gagtagccgg gatggccgga
     6421 tcagccggaa ggaggggcgc ggggcgaaac cccgcgcccc tgccgtccgt atcagagggc
     6481 gggtgaaagc ccgaggttcc ctcctcctcc tcgccgggct ggctatcgtg gccggtccgg
     6541 cgctttgtct ttcggaaggg gcggacgggc tgcgcggcgc cgagatccgg atctcgcgcg
     6601 caaccggtcc gatctcggtg gacggcgacc tcggcgacgc cggctggctc ggggcggcgc
     6661 gggtcgagac cttctacgag acgaacccgg gggacaacct cccgccgaag gtccggacgg
     6721 tcgggcgcct cgcttacgac gaccggttcc tctacgccgc cctcgagtgt gaggaccccg
     6781 agcccgggaa gatccgggcc ccgttctccc agcgggacgt cctcgggagc gggaccgact
     6841 acgccgggat catcctcgac acccgcaacg acggccggac ggcgatcctc ttcctcgcga
     6901 acccgcgtgg gatcccgtac gacgccgtga cggacgacgg caacgaggac tcttcgcccg
     6961 acttccactg ggaggcggcg gggaagatca cggcgacggg ctggaacctc gagatccgga
     7021 tcccgttctc ctcgctccgc tacgaccggg cggacccgca gacctggggg atcctcctct
     7081 acaggaaccg cccgcgcgat tttcgctatc agatgttctc cgcccggctc ccgcgcggac
     7141 ggaactgctt catctgctcg gagaacgtcc tgaccggcct cgaagggctc cccccgggcg
     7201 gacatctcgt cgtggcgccc tacacgaccg cctcgcagga gacgttgccc cgcgcggacg
     7261 ggacggggag cgtggcgaaa cccgtcgagc cggacggcgg cctcgacgtg aaatggaccc
     7321 cctccgccgg cgtggcgctc gacgggacgt tcaaccccga cttctcccag atcgagtccg
     7381 acaccgcccg gatcggcgtg aacgagcgtt tcgcgctctt ctacccggag aagcgcccct
     7441 tcttcctcga gggggtcgac ctcctctcga cgccggttca ggccgtctac acccggtccg
     7501 tcacgtcgcc gcgctggggc cttcgcggga ccggaaagct gggcgggacg agctggacgg
     7561 ccctcgtcgc cgaggaccgc gggggcggga gcgtcatcct tcccggtccg aacggctccg
     7621 atctcgcgga acaggacttc cggtcgctcg acctggtcgg gagggtccgg cgggacgtcg
     7681 gccgctcctt cgcgtcgttc ctcgtcacgg ccaaggagat gcccggctcg gcgagcaacc
     7741 gcgtcctcgg ccccgacttc cgcctgaatc tgagcgagac cgatctggtc accgggcagc
     7801 tcctctggtc gtggagcgag acgccggacc gtcccgacct cgccgccgaa tgggacggcc
     7861 ggtcgctctc gggccacggg gccgacgtct ggtgggagca cacgaccgag acgtgggact
     7921 gggggctcga gtacaaggac ttctccgacg gcttccgggc cgacctcggc ttcgttccgc
     7981 aggtcggctt ccgcgagggg tacgccggag cgggccggac gttccggccg aaggggttct
     8041 tctcccgcgt ccggaccttc ttcttcgccg actacctcgt cgaccgggag ggcgagctcc
     8101 tcagccaggt cgtcgccccg ggcgtcgggt tcgacacgaa gttcaacggc ttcgcccggt
     8161 tcgagctgag gagcgagaag gtccgggcgg ggacccgggt cctcccgcgg aagcgggtcg
     8221 tctactcgct gagcgccgtc ccctcccgat tcttcgccgg gatcgcgatc gacggggcga
     8281 tcggcgagga gatcgacttc gagggggcgc gcacggggaa gggcggatgg ctcgcggcga
     8341 gcgccgtctt ccgcccgggc gaccacctcc ggctcgacct cagcggcgag gtccgctggc
     8401 tggacgtcga cctgcccgac gggcgctcgc cccggctctt caccgcccac gtcgagcggc
     8461 tgaaggcgac gtggaccctc acggcgcgcg cctgggtccg ggtcatcggg cagtacgtgg
     8521 gcgcctggca ggcgccggag ctctacccga ccgaggttga agcggagtcg gggaccttct
     8581 ccgcgtcggc cctcctcggg taccggctga actggcagag cgtcgtcttc ctcggctacg
     8641 gcgacgcccg gacgctctcc gaggacggcg ggctcccgcc ggagagccgc cagctcttcc
     8701 tcaaggtctc gtacgcgttt cagcggtgat cggaaggcgc ccggggcgta gactttcctc
     8761 agcgccagcc gaacgtacga acggaagccg cggccgcgat ggccgggagg acagcatgct
     8821 cgcgcgcgaa cgactcgcga agtgccccgc gtgcaggggg accggcctcg gaggcccctg
     8881 cgcgcgctgc agctcgctcg gccggatcca cttccagggg aacgaggagc agtggatcct
     8941 ctgcggctac tgccggggca ccggcaccgt cgactgcccg cgctgccggc gggagggccg
     9001 catccacttc ttctcgttcc agtgccctcg ctgcggcttc gtggggcccg agtcggaagg
     9061 gcacctcgac gagctctacg ccgacctctg cccgaagtgc gggagctact gacgccgcgc
     9121 gagcggcgaa gcggggcgtc gcgccagctt cagcctcccc gcccggcccg cgccaggacc
     9181 gcttcgccga cgatgccagc ggcctcgtcg atcgggacgc ggtcgacgtt gacgacgacg
     9241 ttgtagagga gcgggtcggc gacgtcgacc tggaagtagc ggcggatgaa gcgccgccgg
     9301 gccttgtcct ccgcctcgac ccaggcgcgg gccgccttct tcgagaggcc ccgcgcctcg
     9361 gcgacgttct cgaggcggct ctcgagcgac gcgacgaggc ggacgtggaa cgcctgcggg
     9421 aggtgcccca ggatcacgtt cgcgccgcgc ccgacgagga cgacgttgcc catctccccg
     9481 aggcgcagga tcgtctccgt cgtctcctgc atcggcttcc aggtggcccg gtggaggccg
     9541 aggagctcct cgatcacgaa cgagaccccc gcgaggtggt cctccggcga ccaggacgcg
     9601 agccggtccg ggagcgcgtc gtcctccagg atcttgtcga cgagctcgcg gtccacgagc
     9661 gcccaggggc cgcgtccctt cggctggtgc tcctcgagcc acgcgcagag cgcctcgccg
     9721 aacgtgttcc cgccggcccc ggtctcgcgt gagacggtga cgaccgggcg ccgcctctcg
     9781 cgcgagcgcg gcggcccctt gggggaggcc ccgaggtgcg cggcgacgaa ggcgagggtc
     9841 ttctcttcgg acgagacgaa ggacatggcg atcctccttc cgaagcgggg cgggtggctt
     9901 caagtgtacg ccgcggggcg catgccggcg ggggagcgcg aagcccagcg cgacgcccgc
     9961 tcgccccgta gagtgtagat ggtagacctg acccctcctc tctctcctct cgggcgtgct
    10021 gcggcgcggc ggatatgatc gcggaccgaa cccgaccgga ttcccgtatg gagctgagga
    10081 aggagcagag gcggatgaag tacgaaggga aggccatcca gaccgttccg ctggacgagg
    10141 gattcgtcga gctgcggttc gacctgcagg gcgactccgt caacaagttc aacagcctca
    10201 ccctcggcga gctgaaggag gtcgtcgcgg ggctgacggc cgcgccgccg aaggggctcc
    10261 tgatcacgag cggcaaggac gtcttcatcg tcggggccga cgtcaccgag ttcctgacgc
    10321 atttcaagcg gagcgaggag gagctcgccg gctggctcct cgagaccgac gcgacgttct
    10381 cggcgatcga ggacctccct tgcccctcgg tcgtcgcgat caacggcttc tgcctcggcg
    10441 gcggcttcga gctcgcgctg tcggcccact tccgcgtcgc ttcgacggcc gcgaaggtcg
    10501 gcgtccccga gacgaagctc gggatcttcc ccggctgggg cggcaccgtc cgcctctcgc
    10561 ggatgtgcgg ggccgacaac gcgatcgagt ggatcgccac gggagagcag taccccgcgg
    10621 ccgaggggct gaagatcggc gccgtcgacg ccgtctgcga gccggagaag ctccgcgagg
    10681 tcgcgctccg gatgctcgcc gacgcggccg acgggaagct cgactggaag aggcgggtcg
    10741 aggagaagaa ggcgccgctc ccgctcaaca aggtcgaggc ggggatggtc ttcgaagggg
    10801 gcaaggcgtt cgtcggcggg aaggccggcc cgaactaccc gtcccccgtc gccgcgatcg
    10861 aggcgatgca gaagggcgcg acgctcgggc gcgacgaggc gctcaagatc gaggcggcga
    10921 ctttcgcgcg gatcgcgaag accccgacgg cccacggcct cgtctcggtc ttcctcggcg
    10981 accagctcgt caagaagctc gcgaagaagg cctccggcgg ggcgaagccg gtgcagcggg
    11041 cggccgtcct cggggccggg atcatggggg gcggcatcgc ctaccagtcg gcctcgaagg
    11101 gggtcccgat cctgatgaag gacatcgccc cgaagcagct cgagctcggg atgggcgagg
    11161 cggccaagct cctcgagaag caggtcgagc gcgggaagat gacggccggg aagatggcgc
    11221 aggtcctcgc cgggatcacg ccgaccctct cctacggcga cttcggcgcg gtcgacatcg
    11281 tcgtcgaggc ggtcgtcgag aacgagaagg tcaagaaatc ggtcctcgcc gacctcgaga
    11341 agtccgtgaa gccggggacg atcctggcgt cgaacacctc gacgatctcg atcacccgac
    11401 tcgccgaggc gctcgcggcc cccgagaact tctgcgggat gcacttcttc aacccggtcc
    11461 cgcggatgcc gctcgtcgag gtgatccgcg gcgcgaagtc gagccccgag gcgatcgcga
    11521 cgacggtcgc gtacgcgacg gcgatgggga agactccgat cgtcgtcggc gactgcgccg
    11581 ggttcctcgt caaccgcgtc ctcttcccct acttcacggg cttcgagctc ctcctcgcgg
    11641 acggcgtcga ctacaagcgg gtcgacaagg tgatggagaa gtggggctgg ccgatgggcc
    11701 cggcgctcct cctcgacgtc gtcgggatcg acacgggcgt ccacgccgac aaggtgatgg
    11761 ccgccgcctt ccccgaccgg atgtcgcacg cgggcgagag cgcgatcgag aagatggtcg
    11821 ccgcccagcg ctacgggcag aagaacggga agggcttcta cgtctgggag ccgcagaaga
    11881 agggcccgcc gaagaagggc gtcgacccgt tcaccgagga ggcgatgaag gcgcacgcga
    11941 aggcctcgcc cgaggtcgca gacgccgaga tcgtcgagcg gatgatgctg ccgatgctcc
    12001 tcgagtgctc gcgctgcctg gaggacggca tcgtcgcgac gccgaccgag gtcgacctcg
    12061 ccctcgtcta cggcctcggc ttcccgccgt tccgcggcgg gatcttccgc tgggccgacg
    12121 ccaccggaac gaaggcgctc ctcgcggccg ccgagaagca cgcgcctctc ggcgccctct
    12181 acgcccccac cgagcagctg aagggcctcg ccgcatccgg caagggcttc cacggagagt
    12241 gatgagatga aagacgtcgt catcgtcgat gccgtccgca ccccgatggg ccgctccaag
    12301 ggcggcatgt tccggaacgt ccgtgccgag aagctctcgg ccgaggtcat caacgcgctc
    12361 ctggcgcgca accccgaggt gaaccccgcc gacgtggacg acgtgatctg gggctgcgtc
    12421 cagcagacgc tcgagcaggc ctacaacgtc gcccgcttcg cccagctcat gacgccgatc
    12481 ccgaagcagg tcccggccca gacgatcaac cgcctctgcg ggtcgtcgat gacggccctc
    12541 cactcggcgg ccatggccat catggccggc tacggcgacc tcttcgtggt cggcggcgtc
    12601 gagcacatgg ggcacgtccc gatgacgcac ggcgtcgact tcgccccgca gaacgccaag
    12661 cggaacgcga aggccgccgg cgcgatgggg ctcaccgccg agtacctcgg ccgcctccac
    12721 gggatcaccc gcgagcagca ggacgccttc gcgtaccgct cgcacatgcg ggcctggcag
    12781 gcgacccagt ccggcgcctt caagcccgag atcgtccccc tcgaggggca cgacgaggac
    12841 ggggcgctga agctcttcga cttcgacgag gtgatccgcc ccgagacgac gctcgagggc
    12901 ctcgccaagc tcaagcccgc tttcgacccg aagagcggga cgatcacggc cgggaactcg
    12961 tccggcatct ccgacggcgc cgcggcgctc ctcgtcatgt cggccgagaa ggcgaaggag
    13021 ctcgggaagc acccgatggc ccgcatccgc tccatgtcgg ccgccggctg cgacccttcg
    13081 atcatgggct acggcccggt cccggcggtg aagaaggcgt tcgcccggat cgggatgaag
    13141 tcgggcgacg tcgacctctg ggagctgaac gaggcgttcg cggcgcagtc gctcccggtc
    13201 ttgaaggacc tcggcctcct cgacgtgatg gaggagaagg tcaacgtgaa cggcggcgcg
    13261 atcgccctcg ggcacccgct cggctgctcg ggcgcccgca tcacggcgac gctcctccac
    13321 gccatgaagg caaagggggc ccgcgtcggg gtcgcgacga tgtgcatcgg cttcggccag
    13381 ggcgtcgcga cggtcttcga gagggtctga cgcgatcggc cggccggacg ggcccgcggg
    13441 ggccgtccgg ccggcgagcc cgcgagagga gggacgaatc ggcatgcggc tcgtgacccg
    13501 gggagacctc gacggcctca cctgctcggt cctcatcacg tccgtggaga aggtcgacgc
    13561 catcgagctc gtccacccgc aggagctgac ggacgggagg ttcgaggtgc gggacgggga
    13621 cgtcctcgcg aacctcccgt tccacccggg ctgctcgatc tggttcgacc accacgagct
    13681 cgtcgacgcg aacccgcggc ccgaggcggc cttccagggc cgctacggcc tcgccccgtc
    13741 gacggcgcgc ctcgtccacg actattactg cgagcggaag gggtggcggc tcttcgagcg
    13801 gtacggggag ctcgtcgccg ggacggatcg cctcgactcg gcgaacctga cccgccgcga
    13861 cgtcctcgac ccgcagcggg tgatcctcct gggcttcacg atcgactcga ggaccggcct
    13921 cggcggcttc cgggactact tcctcttcct cgggatggcg ctccgctcga tgccgctcga
    13981 cgaggtcctc cgctcgccgc aggtgacgga gcgcgtcgcg aagatgaagg aggccgacga
    14041 ggagttccgc agggcgctcc tcgccagctc tcacgtcgac gggaacgtgg tcgtg
//
LOCUS       DAOTEZ010000059        14050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3001_length_14050_cov_6.982208, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000059 DAOTEZ010000000
VERSION     DAOTEZ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14050
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3001_length_14050_cov_6.982208"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1421)
                     /locus_tag="P5164_04765"
     CDS             complement(<1..1421)
                     /locus_tag="P5164_04765"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRY43240.1"
                     /translation="MTLRLRVVSADGRRFDHVVEGDALVIGRSSRADLALADRAMSRE
                     HARFRRDEAGWWVEDLGSHNGTRLNEVPLDGAHRVHDGDTVSAGGSVLVAEIHGDGDT
                     GSGDSVIYRSARELLEAVSGSTDASGIGGSGKKAAERLHMLNGVNQALASSISLEELL
                     ELILDRAFEHLRPQEAAIFLRDPSGVDVCAARRTTPGREALPVHSKSLFHEVIDKGMA
                     AHVVDSAADSRFAASRSLILSGLRSFLAAPLLDAQGALGMIVVGAALGVRSFKSEDLE
                     LLVSLASVAALRIRNVRLVAEAMERQRLEQEVRLARQIQVALLPATLPQLPGWELHGG
                     TLPSRGVSGDFYKLLLRGDGASCALFVADVSGKGIAASLLTASLEALSALPLEGSDPP
                     ARICEQVGALLHRRTPPEKYATAFLGVFEPASGRLSWTNAGHNPALLLRAGGEAEWLR
                     SNGVPLGLIPGAKYGEGELVMEPG"
     gene            complement(1454..2254)
                     /locus_tag="P5164_04770"
     CDS             complement(1454..2254)
                     /locus_tag="P5164_04770"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HRY43241.1"
                     /translation="MPNAATAAAPDPAAVDAAARILRRARSVLFVTGAGISADSGLPT
                     YRGVGGLYESDATEEGLPIEVLLSGETFRRRPATTWKYLGAIERACRGARPNRAHEAI
                     AALEGALPRVVVLTQNIDGLHRAAGSRDVIEIHGDVHRLACTRCAFRARVPDYEGFEM
                     PPRCPECAALVRPEVVLFGEMLPDEAVARLEEELRRGFDAVFSIGTTSVFPYVAAPVR
                     MARAWGAGTVEVNPGETEVSGIVDVRLRCGAAAAMDALLSFVERSPVG"
     gene            2334..3050
                     /locus_tag="P5164_04775"
     CDS             2334..3050
                     /locus_tag="P5164_04775"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRY43242.1"
                     /translation="MPKPGQRRHPRYEVQNVHGSLLYRTTVKVRNVSVSGLSLESAER
                     LQLGRAYAIRLAGAGEAVDVHGTIRWCHLSSARPRPGGETRAVYEAGLAFDDVFTGSG
                     RSLLGFLEHHVVLSPQQRLTGRFRADALLPAALEARDEFEVLRLSLSGMLVRTALEAS
                     VESRFALELRLRSGTVPLEGRVAFVQREEAEKGSVAAQLGIEFVGGTDEARGAVARFI
                     AEELERGPGDPPDGSGPAAS"
     gene            3084..3848
                     /gene="xth"
                     /locus_tag="P5164_04780"
     CDS             3084..3848
                     /gene="xth"
                     /locus_tag="P5164_04780"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRY43243.1"
                     /translation="MKIATWNVNSVKARRERLLAFLARHAPDVVCLQELKTEEKGFPF
                     EEVRAAGYFAAVLGQKTYNGVAVLSREEPADVSTGFGDGGDESQARLVAATVGGLRIA
                     SAYIPNGKEVGSEKWPYKLEWLARLAAWVERQSGGPPLVLGGDFNIAPEDRDVASPAS
                     WADSVLCHPEVRSAWERILSAGLVDSFRLHHAEGGLYSWWDYRMLAFPKNEGLRIDHV
                     LLAPALVPRCTGAFIDRDERKGKLPSDHAPVFAELS"
     gene            complement(3897..4748)
                     /locus_tag="P5164_04785"
     CDS             complement(3897..4748)
                     /locus_tag="P5164_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43244.1"
                     /translation="MSVDVPVDPAPLAAPQRRLTTLLDAALVVGSSAVLLAWIAGRPI
                     LYEAASPVMSAFTALSLLLMALVRQARLHVGTWPMALSLAMSGLVLGGNVSSIVMLAF
                     LPPGLWASFSGVVLTSTMTSIGLILFCLHDLAVELRETPESPFLLDDLLIHLALVPGG
                     VSLLGYLLGNPTYLSVHADPRVGISPLEMAFLATYAASAILSNPRLFLWRFLAAGWTN
                     RLVFAGLFANQFVAPVVVAFLFSERRPRGPGLELFAMLGGVVATLTFLLLQARLQRRR
                     AAAHATG"
     gene            complement(4834..5715)
                     /locus_tag="P5164_04790"
     CDS             complement(4834..5715)
                     /locus_tag="P5164_04790"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRY43245.1"
                     /translation="MTNEPRVRRRPPLAPLALLALAAASATAPARPPAPTPQAEAPAA
                     TARRALAERDVPFEAHAFVSAAADGDLPLVELFLDAGMPASTRDRFGVSALVEAARAD
                     RPEILALLLSRGAKPGEAQGGEPPLVAAAEAGSLRTLSTLLSAGADPDQPADPAKGPG
                     GTALQRACAAEAWEAARLLLEGGASATAAAPSMPSPLFLAAESGHAPTVALLLAKGAD
                     PNVRGPGGAAPIWLPVWNGHVEVVRLLLLAGADVRSDRPTLLKGRPGHPVKPEIRRLL
                     RTPPKPVATKRPAPGPR"
     gene            5779..7374
                     /locus_tag="P5164_04795"
     CDS             5779..7374
                     /locus_tag="P5164_04795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRY43246.1"
                     /translation="MARSPLCRRLARALRLASLPEGADPASGGRANASRLSRPSRRDA
                     LRAGGVALAGLAAARCASVVPGAAHSGPDEVLVVGGGLAGLTAAYRLKQAGVAVRVLE
                     AQERIGGRMRSLTGHFPEGLVAELGAELIDTSHESLRRLAAELGLELDDLLVEPPGVT
                     ETLFFAGARRSEAEMVEAMRPVVAAARRDLALLGEEPDASYQGPEAARALDALSIPAW
                     LEKHGISGWARTLVEVAFESECGLPPGDQSCLNFLLFASFDPEHPKLYGESDERYHVR
                     GGNDQVPTLLAGKLDDAVLRGTRLEALRTGSDGRYVATVRREGVSREERASHVVLALP
                     FTLLRDVRLDVPLSPAKKRAIAELGYGTNAKLMTGWTRREWRLAHGSTGTVMTDAGFQ
                     VAWETSRAQKGSAGVLTNFTGGAEGLAVGQGTAQERAVELAGRLDRVFPGLSAHHRAS
                     EAVRFHWPTHPWTRGSYACYRVGQITAFGGAEAEREGGVFFAGEHTSADFQGFMEGAC
                     ESGERAAREVLSAMGLSERAPAA"
     gene            7475..8917
                     /locus_tag="P5164_04800"
     CDS             7475..8917
                     /locus_tag="P5164_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRY43247.1"
                     /translation="MTTATLTWIGLGLYVLVAAGFAIRGARKSGSVASYAVGSRDLPA
                     WVVGLSLSAQLTSVATFVVNPGLVAHFGLAALLGYGVAAGSGITIGLAVLSGPFRETG
                     ARVMALTIPQWIGTRWESKGLRLAFVVLSAGLLAFATLIVVALALVLASLLGVPAVAV
                     AAAVLVYVLLAMAMGGATAHAWINAVQALIMIVVALLLVGTAFGQAPPLGGVLERLAT
                     IQPSLATAVNPASPYFRSLFEVFFCNFLVGLAIVGQPHILSKALYLSDGKQVRRYLAV
                     AIGAGLVFMAVLWVGLAARLSLPTLPRIDLVVPSWIGASFGPGLSVVVAIGIVCAGLS
                     TLEGIFLALASILSVDVHPLLPGAKPEKALRFGRLGLAAVALVCLLLARWQIAHPTGG
                     SVAIFAQYGIYLLFTASSVPLVAGMFLPRARRGGVALAAAAAVVVYVAAGTLKLGVLS
                     NNPAVLATWGLLASWVVLGASLLVPGRREA"
     gene            8959..10281
                     /locus_tag="P5164_04805"
     CDS             8959..10281
                     /locus_tag="P5164_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HRY43248.1"
                     /translation="MRPLNALVERYLPSALVFAVVLTAVVALLALLLTDAGPAEVLRG
                     WGDGLSGLLAFTTQMALILMLGHALANTRPVRALLARLGSLPRTPRQAYVSVFLVAAA
                     ASLVTWGLGLVVGALLAREVAAQGRARGLRLHFPLLVAAGFSGFVVWHMGSSGSGPLT
                     AATEGSFIATQLGRTVPVSATTFAPWNLLATVATIAAVAAALALAAPREGDPVREVEA
                     DLRDAGPRVEEVVVTPADRLDASRIVTSAVGLALAAWLALHFARGGSLTLDTVNWTFL
                     ALVCLLVRSPFELIALVKGAASTVGDILLQFPLYAGILGIMTTTGLIAVLSDAFVGLA
                     SPRTFGFLSLLSAGLVNFFVPSGGGQFAVQGPILLDAAARIGVAPEVAIMALAYGDQW
                     TNMVQPFWALPLLAIAGLKVRDILAYTTVTLLASGLVFAATLLIVGAG"
     gene            10499..10957
                     /locus_tag="P5164_04810"
     CDS             10499..10957
                     /locus_tag="P5164_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43249.1"
                     /translation="MSYRNSLVERAARDPLHEDGERLVVCVAVGPALPRRETARRRRP
                     ESEECRLRRLRPVAERPVAVQPEEVGEPRRVGQEVRHAELGVVCEPLREVIANGGVEA
                     DQPLVGEREDGRGGELLRDRPDGEDGAGCRGPLAVADGAVALLEDDLAVS"
     gene            complement(11220..11975)
                     /locus_tag="P5164_04815"
     CDS             complement(11220..11975)
                     /locus_tag="P5164_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006919994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY43250.1"
                     /translation="MDGRTNLVSGLLLLAALAPLAGVALAAGPGLEKDVIPTSAGPLE
                     VAFVGHGSLFFRFGGKVFHVDPYGRLADYATLPKADVVLLTHAHGDHLDPAALAAVRT
                     ATTMLVVAPVCTGKVEGATVLPNGEATTVEGVRVEAVPAYNLVAKRPDGSPFHPKGEG
                     NGYVLTFGKTRVYVAGDTESVPEMKALEGIDVAFLPMNLPYTMTPEMVADAVRAFRPR
                     VLYPYHYGETDPQKLVALLAEEKGIEVRVRKMR"
     gene            complement(12108..12707)
                     /locus_tag="P5164_04820"
     CDS             complement(12108..12707)
                     /locus_tag="P5164_04820"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HRY43251.1"
                     /translation="MRSPRHLLSFGIAVPILYLLALVLGSALYPGYSHVTRYASELGA
                     AEAPHPWIFNSGIVAAGVAALLAGPGFGVALRRLGSDRVRAVLSGLAVSLWGVAMVLG
                     GSFPMPDPRHGGFGLGLGLQVAPLFLLWGLRRAPDAGRLRLFLLVVFLAMTALFAVMM
                     GVGGLVTRANVGLWQRGNALVSIPWIGILACALRRRLAR"
     gene            complement(12844..13290)
                     /locus_tag="P5164_04825"
     CDS             complement(12844..13290)
                     /locus_tag="P5164_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRY43252.1"
                     /translation="MLLFDVNVLVHAHRRDAPDHTKYRKWLEATLGADSAYGVSDLVL
                     SGFLRVVTHPNVFREPTPLESALAFVHEVRDRANAVEIVPGPRHWEIFTRLCRECGVR
                     GNLVPDAWLAALAVESGCEWVTTDRDFARFPGLRWRHPLQGTRRPG"
     gene            complement(13271..13528)
                     /locus_tag="P5164_04830"
     CDS             complement(13271..13528)
                     /locus_tag="P5164_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020396251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2191 domain-containing protein"
                     /protein_id="HRY43253.1"
                     /translation="MRTTIHLPDPLLDQARRRALETGTTLTGVIEDALRESLAGKRTG
                     KKASPVRLTTFGGKGLRPGVDLDDSAALLDVMEEPRAPVRR"
     gene            complement(13563..>14050)
                     /locus_tag="P5164_04835"
     CDS             complement(13563..>14050)
                     /locus_tag="P5164_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HRY43254.1"
                     /translation="SPLPEMSRFEETVSDYQTTELTAGPHLIAHFRAQLKREGVLSAA
                     DLMRAPDGATVKTAGVIVVRQRPGTAKGFVFLSLEDETGMCQAILRPDLFREHRSLVV
                     GSPALVVEGRLQKKDGTLSVRAERFERMSLDEPIPELRKPVSPPYPRHRPPTPPSHDF
                     R"
BASE COUNT         1829 a         5021 c         5319 g         1881 t
ORIGIN      
        1 cccggctcca tcacgagctc gccttccccg tacttcgccc ccgggatgag cccgagcggg
       61 acgccgttcg accgaagcca ttccgcctcc cctccggcgc gcagcaggag cgcgggattg
      121 tgtccggcgt tcgtccagga gaggcgcccc gaggccggct cgaagacgcc gaggaaggcc
      181 gtggcgtact tctcgggcgg cgtccgacgg tggaggagcg ccccgacctg ctcgcagatc
      241 cgggccggcg ggtccgatcc ctcgagaggg agcgcggaaa gcgcttcgag tgaagccgtc
      301 agcagcgagg cggcgatccc cttgcccgag acgtccgcca cgaagagcgc gcacgacgcc
      361 ccgtcgccgc ggaggaggag cttgtagaag tcgccggaga ccccgcgcga ggggagcgtc
      421 cctccgtgga gctcccagcc ggggagctgc ggcagcgtcg ccgggaggag ggcgacctgg
      481 atctgccgcg cgaggcgcac ctcctgctcg aggcgctggc gctccatggc ctcggccacg
      541 aggcggacgt tccggatgcg gagcgccgcc accgaggcga gcgagacgag gagctcgagg
      601 tcctcgctct tgaagctcct cacgccgagg gccgcgccga cgacgatcat cccgagggcc
      661 ccctgggcgt cgaggagagg ggcggcgagg aagctccgga gcccggagag gatcaggctt
      721 cggctcgcgg cgaagcggga gtcggccgcg gagtcgacca cgtgggcggc catccccttg
      781 tcgatcacct cgtgaaagag gctcttcgag tgcaccggga gagcctcgcg ccccggcgtc
      841 gtccggcggg cggcgcagac gtcgactccc gaagggtcgc gcaggaagat cgccgcttcc
      901 tgcggccgga ggtgctcgaa cgcccggtcc aggatcagct cgaggagctc ctcgagcgag
      961 atggagctcg cgagggcctg gttcacgccg ttgagcatgt ggagacgctc ggctgccttc
     1021 ttccccgagc ccccgatccc ggaggcgtcg gtggagccgg acaccgcctc gaggagctcg
     1081 cgggcggagc ggtagataac ggagtccccg ctcccggtgt cgccgtcccc gtggatctcc
     1141 gcgacgagga cgctccctcc ggcggagacc gtgtcgccgt cgtgcacccg gtgcgctccg
     1201 tcgaggggca cctcgttcag ccgggtcccg ttgtgcgagc cgaggtcctc gacccaccag
     1261 cccgcctcgt cgcgccggaa ccgggcgtgc tcgcgggaca tggcgcgatc cgcgagggcg
     1321 aggtccgccc gggaggagcg gccgatgacg agggcgtcgc cctccaccac gtgatcgaag
     1381 cggcgaccgt cggcagagac gaccctcaat cggagcgtca tgcgttccct cgtgctgtcc
     1441 gttccgggtc agtctatccg acgggcgacc gctccacgaa ggaaaggagc gcgtccatgg
     1501 cggccgccgc gccgcagcgc agacggacgt cgacgattcc ggagacctcg gtctcgcccg
     1561 gattcacctc gaccgtcccc gcgccccacg cgcgagccat ccggacgggc gcggcgacgt
     1621 aagggaagac gctcgtcgtc ccgatggaga agacggcgtc gaagccgcgt cgaagctcct
     1681 cctccaggcg ggcgaccgcc tcgtcgggga gcatctcgcc gaagaggacg acctccggcc
     1741 ggacgagcgc ggcgcactcg gggcagcggg gaggcatctc gaagccctcg tagtccggga
     1801 cgcgagccct gaaggcgcag cgggtgcagg cgagccggtg gacgtcgccg tggatctcga
     1861 tgacgtcccg cgagccggcg gcccggtgga ggccgtcgat gttctgggtg aggacgacga
     1921 cgcgcgggag agcgccctcg agcgcggcga tcgcctcgtg ggcccggttc ggccgggcgc
     1981 cgcggcaggc gcgctcgatg gcgccgaggt acttccaggt cgtcgccggc cttcgccgga
     2041 acgtctcgcc ggagaggagc acctcgatcg ggagcccctc ctccgtcgcg tccgactcgt
     2101 agagcccgcc gacgccccgg taggtcggga ggcccgagtc ggcggagatc ccggccccgg
     2161 tgacgaagag gacggagcgg gcccggcgga ggatccgggc ggcggcgtcg acggcggcgg
     2221 ggtccggcgc ggcggccgtc gcggcgttcg gcacgacgac ccagtgtacg gcccgggcgc
     2281 agcggcgcga taatcgcggg gccagcggca cagcagcgga ggacgacgag agcatgccga
     2341 agcccggcca gcgccgccat ccgaggtacg aggtccagaa cgtccacgga tctctcctct
     2401 acaggacgac ggtgaaggtc cgcaacgtga gcgtgtcggg gctctcgctc gagtcggcgg
     2461 agcggctcca gctgggccgc gcctacgcga tccggctcgc cggggcgggc gaagcggtcg
     2521 acgttcacgg gacgatccgc tggtgccacc tctcgagcgc gcggccgcgc cccggcggcg
     2581 agacgcgcgc ggtctacgag gcgggcctcg cgttcgacga cgtcttcacc gggagcggcc
     2641 gctccctcct cggtttcctc gagcaccacg tcgtcctctc cccgcagcag cggctgacgg
     2701 ggcgcttccg ggccgacgcg ctcctcccgg cggcgctcga ggcgcgggac gagttcgagg
     2761 tcctgcggct gagcctctcc gggatgctcg tgcggaccgc cctcgaggcg tccgtggaga
     2821 gccgcttcgc cctggagctc cgcctccggt ccgggacggt ccctctcgag gggcgggtcg
     2881 cgttcgtgca gcgcgaggag gcggagaagg gctcggtggc ggcgcagctc ggcatcgagt
     2941 tcgtcggcgg cactgacgag gcgcgcggcg cggtcgcccg gttcatcgcc gaggagctcg
     3001 agcgcgggcc gggcgacccg ccggacggct ccggccccgc ggcctcctag aatcgcgccg
     3061 cgcccggcgg cgcgggaggc ccgatgaaga tcgccacctg gaacgtgaac tcggtgaagg
     3121 cccgcaggga acggctcctc gcgttcctcg cgcggcacgc ccccgacgtc gtctgcctgc
     3181 aggagctgaa gacggaggag aagggcttcc cgttcgagga ggtccgcgcc gcgggctact
     3241 tcgccgcggt cctcgggcag aagacgtaca acggggtggc cgtcctctcc cgcgaggagc
     3301 cggcggacgt ctcgaccgga ttcggcgacg gcggcgacga gagccaggct cgcctcgtcg
     3361 cggcgacggt cggcggcctg cggatcgcgt cggcctacat cccgaacggc aaggaggtcg
     3421 gctcggagaa gtggccctac aagctcgagt ggctcgcgcg cctcgcggcg tgggtcgagc
     3481 gccagtcggg cggcccgccg ctcgttctcg gcggcgactt caacatcgcc ccggaagacc
     3541 gggacgtcgc gagccccgcc tcgtgggccg attccgtcct ctgccacccc gaggtccgct
     3601 ccgcgtggga gaggatcctc tccgccggcc tcgtcgactc gttccgcctc catcatgccg
     3661 agggcgggct ctactcctgg tgggactacc ggatgctcgc gttcccgaag aacgagggcc
     3721 tccggatcga ccacgtcctc ctcgcgcccg cgctcgtccc gcgctgcacc ggggcgttca
     3781 tcgaccgcga cgagaggaag gggaagctcc cctccgacca cgcgccggtc ttcgccgagc
     3841 tctcctgagc cctccgggca cgccgcgtcc ggcgtcgtcg gacgggagag aggggctcag
     3901 cccgtcgcgt gcgcggcagc gcgccggcgc tggagccgcg cctgcaggag gaggaacgtg
     3961 agcgtcgcga cgacgccgcc gagcatcgcg aagagctcga ggccggggcc gcgcgggcgc
     4021 cgttcggaga agaggaaggc gacgacgacg ggcgcgacga actgattcgc gaagagcccg
     4081 gcgaagacga ggcggttcgt ccacccggcg gcgaggaacc tccagaggaa gagccgcggg
     4141 ttcgagagga tcgcgctcgc ggcgtaggtc gcgaggaagg ccatctcgag cggcgagatc
     4201 ccgacgcgcg ggtcggcgtg gacgctcaga taggtcgggt tcccgaggag gtagccgagg
     4261 aggctgacgc cgccggggac gagcgcgagg tggatcagaa ggtcgtcgag aaggaagggc
     4321 gactcgggag tctcccggag ctcgacggcg aggtcgtgga ggcagaagag gatcaggccg
     4381 atggaggtca tcgtcgacgt caggacgacg ccggagaagc tcgcccagag gccgggcggc
     4441 aggaacgcca gcatcacgat cgaggagacg ttgccgccaa ggacgaggcc gctcatcgcg
     4501 aggctgagcg ccatcggcca ggtcccgacg tggaggcgcg cctggcggac gagggccatg
     4561 aggaggaggg agagcgccgt gaacgccgac atcaccggcg acgccgcctc gtagaggatc
     4621 gggcgcccgg cgatccacgc gaggaggacg gcggagctcc cgacgacgag tgcggcgtcg
     4681 agcagcgtcg tcaggcgccg ctggggcgcg gcgagcgggg cggggtcgac gggaacgtca
     4741 acggacatct cgggagagct cctcggcggc gatttccgcg gaccataccc cggacgacgg
     4801 cgggcgcgtc ggcgccgccg gatcggacgc tcttcagcgc ggcccgggcg ccggccgctt
     4861 cgtcgcgacc ggcttcggag gggtccggag gagccggcgg atctcgggct tcaccggatg
     4921 gccggggcgt cctttcagga gcgtcggccg atcggagcgg acgtcggcgc cggcgaggag
     4981 gagaagccgt acgacctcga cgtgtccgtt ccagaccggg agccagatcg gcgccgcgcc
     5041 gcccgggccg cggacgttcg ggtcggcccc cttcgcgagg agaagggcga cggtcggggc
     5101 gtgtccgctc tcggcagcga ggaagagcgg ggacggcatc gacggcgcgg cggccgtcgc
     5161 gctcgcgccc ccctcgagaa ggagccgggc cgcctcccac gcctcggcgg cgcaggcgcg
     5221 ctgcagcgcc gttccgcccg gccccttcgc gggatcggcc ggctggtcgg ggtcggcgcc
     5281 ggcggagagg agcgtcgaga gcgtccgcag gctccccgcc tccgccgccg cgacgagcgg
     5341 cggctcgccc ccctgcgcct cgcccggctt cgcgccgcgc gagaggagga gcgcgaggat
     5401 ctcgggccgg tcggcgcggg cggcctcgac gagggcgctg acgccgaagc ggtcgcgcgt
     5461 cgacgcgggc atcccggcgt cgaggaagag ctcgacgagc gggaggtcgc cgtccgccgc
     5521 cgccgagacg aacgcgtgcg cctcgaatgg gacgtcccgc tcggcgagcg cacggcgggc
     5581 cgtcgcggcg ggtgcctccg cctgcggcgt gggggcgggc ggacgggcgg gcgccgtcgc
     5641 ggaagccgcg gcgagggcga gcagggcgag cggcgcgaga ggcggtcggc ggcggacgcg
     5701 aggctcgttc gtcatcgtct gcggtgcgca ttctgacccg gctagaatcc cggcacccca
     5761 caaatcggag gtctcccgat ggcccgcagc ccgctctgcc gacgcctcgc ccgcgccctg
     5821 cgcctcgcat cgctccccga gggagccgac cccgcttcgg gcgggcgcgc aaacgcgtcc
     5881 cgtctctccc ggccgtcgcg ccgggacgcg ctccgtgcgg gaggtgtcgc gctggccggg
     5941 ctcgccgcgg cgcgctgcgc ctccgtcgtc ccgggggcgg cgcactcggg gccggacgag
     6001 gtcctcgtcg tcgggggcgg gctcgccggc ctgacggcgg cctatcgcct gaagcaggcg
     6061 ggagtcgcgg tccgcgtcct cgaggcccag gagcggatcg gcgggaggat gcggagcctg
     6121 acggggcact tcccggaggg gctcgtcgcc gagctcggcg cggagctgat cgacacgtcc
     6181 cacgaaagcc tccgccgcct cgcggccgag ctcggcctcg agctggacga cctcctcgtc
     6241 gagccgccgg gggtgacgga gacgctcttc ttcgccggcg cgcgccggag cgaggccgag
     6301 atggtcgagg cgatgcgccc cgtcgtcgcg gcggcgcggc gcgacctcgc gcttctcggc
     6361 gaggagcccg acgcctcgta tcaggggccg gaggcggccc gggccctcga cgccctctcg
     6421 atcccagcgt ggctcgagaa gcacgggatc tccggctggg cgaggacgct cgtcgaggtg
     6481 gccttcgagt cggagtgcgg cctgccgccc ggtgaccaga gctgcctcaa cttcctcctc
     6541 ttcgcgagct tcgacccgga gcacccgaag ctctacggcg agagcgacga gcgctaccac
     6601 gttcgcggcg ggaacgacca ggtgccgacg ctcctggccg ggaagctcga cgacgccgtc
     6661 ctccgcggga cgcggctcga ggcgctccgg accgggagcg acgggcggta cgtcgcgacg
     6721 gtccgacgcg agggggtcag ccgcgaggag cgcgcctccc acgtcgtcct cgccctgccg
     6781 ttcacgctcc tgcgcgacgt gaggctcgac gtcccgctct cgccggcgaa gaagcgggcg
     6841 attgccgagc tcggctacgg gacgaacgcg aagctgatga ccggctggac gcggcgtgag
     6901 tggcggctcg cgcacggctc gacggggacg gtgatgaccg acgccggctt ccaggtcgcg
     6961 tgggagacga gccgcgcgca gaagggctcg gcgggcgtcc tgacgaactt caccggcggc
     7021 gccgaggggc tcgccgtcgg gcaggggacg gcccaggagc gggccgtcga gctcgccggg
     7081 cgcctcgacc gcgtcttccc cggcctctcg gcgcaccacc gcgcctccga ggcggtccgc
     7141 ttccactggc cgacgcaccc gtggacgcgc ggcagctacg cctgctaccg cgtcggccag
     7201 atcacggcgt tcggcggagc cgaggccgaa cgggaaggag gcgtcttctt cgcgggcgag
     7261 cacacctccg cagacttcca gggcttcatg gaaggggcct gcgagtcggg ggagcgcgcg
     7321 gcgcgggagg tcctctcggc gatgggcctt tcggagcggg cgccggcggc ctgacccgcc
     7381 gccgcccggc tcctcgcgcg cgggcccggg cccgtcgcat ctatcatccg cccgcgggcg
     7441 gcgcgcctcc ggcacgccgc gggaggagcg agtgatgacg acggcgacgc tgacctggat
     7501 cggtctgggt ctctacgtgc tcgtggcggc gggattcgcg atccgggggg cgcgcaagtc
     7561 cggaagcgtc gcctcgtacg ccgtcgggag ccgggacctc ccggcgtggg tcgtcggcct
     7621 ctccctttcg gcccagctga cgagcgtcgc gacgttcgtc gtcaaccccg gcctcgtcgc
     7681 gcacttcggc ctcgccgcgc tcctcggcta cggcgtcgcc gcggggagcg ggatcacgat
     7741 cggcctcgcc gtcctctcgg ggccgttccg ggagacgggg gcccgcgtga tggcgctgac
     7801 gatcccgcag tggatcggga cccgctggga gtcgaagggg ctgcgcctcg cgttcgtcgt
     7861 cctgtcggcc ggcctcctcg ccttcgcgac gctcatcgtc gtcgccctcg cgctcgtcct
     7921 cgcgagcctc ctcggcgtcc cggccgtggc cgtcgccgcg gccgtcctcg tctacgtcct
     7981 cctcgcgatg gccatggggg gcgcgacggc gcacgcgtgg atcaacgcgg tccaggccct
     8041 catcatgatc gtcgtcgcgc tccttctcgt cgggacggcg ttcggccagg cgcccccgtt
     8101 gggcggcgtc ctcgagcggc tcgcgacgat ccagccctcg ctcgcgaccg ccgtgaaccc
     8161 ggcctccccc tacttccgga gcctcttcga ggtcttcttc tgcaacttcc tcgtcggcct
     8221 cgcgatcgtc gggcagccgc acatcctctc gaaggcgctc tacctctccg acgggaagca
     8281 ggtgcgccgg tacctcgccg tcgcgatcgg cgccgggctc gtcttcatgg cggtcctctg
     8341 ggtcggcctc gcggcgcgcc tctcgctgcc gacgctcccg cggatcgacc tcgtcgtccc
     8401 gagctggatc ggcgcctcct tcggccccgg cctgagcgtc gtcgtcgcga tcggaatcgt
     8461 ctgcgccggg ctctcgacgc tcgaggggat cttcctcgcc ctcgcgagca tcctctcggt
     8521 cgacgtccac ccgctcctgc cgggggcgaa gccggagaag gcgctccgct tcgggcggct
     8581 cggcctcgcg gcggtcgccc tcgtctgcct cctcctcgcc cgctggcaga tcgcccaccc
     8641 gaccgggggg tccgtcgcga tcttcgctca gtacggcatc tacctcctct ttaccgcctc
     8701 ctccgtcccg ctcgtcgccg ggatgttcct gccgcgggcg cggcgcggcg gcgtcgccct
     8761 cgcggccgcg gcggcggtcg tcgtctacgt cgccgccggg acgctgaagc tcggggtcct
     8821 ctcgaacaac ccggccgtcc tcgcgacgtg ggggctcctc gcctcctggg tcgtcctcgg
     8881 agcctcgctc ctcgtcccgg ggcggcgcga ggcatgagcc cgtcgggcgc tccgcggccc
     8941 ggcgggggcg cggccctcat gcgaccgctg aacgcgctcg tcgagcgcta ccttccgagc
     9001 gccctcgtct tcgccgtcgt cctgacggcg gtcgtcgccc tcctcgccct cctcctgacc
     9061 gacgccggcc ccgcggaggt cctccgcggc tggggcgacg ggctctcggg cctcctcgcg
     9121 ttcacgacgc agatggcgct gatcctgatg ctcgggcacg cgctcgcgaa cacgcgcccc
     9181 gtgcgggcgc tcctcgcccg gctcgggagc cttccgcgga cgccgcgcca ggcctacgtc
     9241 tcggtcttcc tcgtcgcggc ggccgcctcg ctcgtgacgt ggggcctcgg cctcgtcgtc
     9301 ggggcgctcc tcgcccgcga ggtggcggcg caggggaggg cgcgcggcct gaggctccac
     9361 ttcccgctcc tcgtcgccgc gggcttctcc ggcttcgtcg tctggcacat gggctcctcg
     9421 ggctccggac cgctcaccgc ggcgacggag gggtccttca tcgcgacgca gctcggccgg
     9481 accgtgccgg tctcggcgac gacgttcgcc ccctggaacc tcctcgccac ggtcgcgacg
     9541 atcgcggccg tcgcggcggc cctcgccctc gcggcgcccc gcgaaggcga cccggtccgc
     9601 gaggtcgaag cggacttgcg cgacgccggg ccgcgggtcg aagaggtggt cgtcacgccg
     9661 gccgaccggc tcgacgcgag ccgcatcgtc acctcggcgg tcgggctcgc gctcgcggcc
     9721 tggctcgcgc tccacttcgc gcgcggcggc tcgctgacgc tcgacaccgt gaactggacg
     9781 ttcctcgccc tcgtctgcct cctcgtgagg agccccttcg agctgatcgc gctcgtgaag
     9841 ggagcggcct cgacggtggg cgacatcctc ctgcagttcc cgctctacgc cgggatcctc
     9901 gggatcatga cgacgacggg gctcatcgcc gtcctctccg acgccttcgt cggcctcgcg
     9961 tcgccgcgca cgttcggctt cctctcgctc ctctccgccg gcctcgtgaa cttcttcgtc
    10021 ccgtcgggcg gagggcagtt cgccgtccag gggccgatcc tcctcgacgc ggccgcgcgg
    10081 atcggcgtcg cccccgaggt cgcgatcatg gcgctcgcct acggcgacca gtggacgaac
    10141 atggtccagc cgttctgggc gcttccgctc ctcgcgatcg cggggctgaa ggtgcgcgac
    10201 atcctcgcct acacgacggt cacgctcctc gcgtcgggcc tcgtcttcgc cgcgacgctc
    10261 ctgatcgtcg gggcagggta gggcggggcc gggctaacgc gtttccgcgg tccggcgggc
    10321 gtaggagcgg acctcgctcc agtccacctc gaaccggtcg accgacccgt ccggcggcag
    10381 gcggaggagg aacgaggccg ggtcgtcgcg cgagacccag cggcgctcgc ccgccggccg
    10441 gaagacgagc ggcccgatca gatcgatctc gacgcgcagg tcgtccccct cggcggagat
    10501 gtcgtaccgg aacagcctcg tcgaacgggc ggcccgcgac ccgctccacg aggacggcga
    10561 gcgccttgta gtttgtgttg ctgtaggccc agcgctcccc cggcgggaaa cggcgcggcg
    10621 ccgccgcccc gagagcgagg aatgccgtct tcggcgtctc cgcccggtcg ccgagcgccc
    10681 ggtagccgtc cagccagagg aagtcggcga gccccgacgt gtgggtcagg aggtgcgaca
    10741 cgcggagctc ggggtcgtgt gcgaaccact ccgggaggta atcgcgaacg ggggcgtcga
    10801 ggcggaccag cccctcgtcg gcgagcgcga ggacggccgc ggcggtgaac tgcttcgaga
    10861 tcgacccgat ggggaagacg gagcggggtg tcgggggccc ctcgccgtcg cggacggagc
    10921 cgtagccctt ctcgaagacg acctcgccgt gtcttaggac cgtcacgtgc gcggcccgga
    10981 cgccttcgcg cgcgaggaac gcgcggaccg tctcgtccag ctttccgagg tcgggcgtcg
    11041 gggtcgtcgc ccgcgggcgg tcggcagcaa ggggcctcgg gtcgataccg ccatcgcggg
    11101 cgcaagccgc cgcgagagcc gtcaccagga ggaacgtcgc acgggccgtc cttcgtctca
    11161 cgaggcctcc ctgccgtccg ggccggtctg tggcgggagg atacgggaga ccgggcgagc
    11221 tacctcatct tccggacgcg gacctcgatc cccttctcct ccgcgaggag agcgacgagc
    11281 ttctgcgggt cggtctcgcc gtagtgatat gggtagagga cgcgcggacg gaatgcgcgg
    11341 acggcgtcgg cgaccatctc gggcgtcatc gtgtagggga ggttcatcgg gaggaaggcg
    11401 acgtcgatcc cctcgagcgc cttcatctcc gggacgcttt ccgtgtcgcc ggcgacgtag
    11461 acgcgggtct tcccgaacgt caggacgtag ccgttcccct cgcccttcgg gtggaacggg
    11521 gagccgtcgg gccgcttcgc gacgaggttg taggccggga ccgcctcgac gcggacccct
    11581 tcgaccgtcg tcgcctcgcc gttcggaagg acggtcgctc cctcgacctt ccctgtgcag
    11641 acaggggcga cgacgagcat cgtcgtcgcc gtccggaccg ccgcgagcgc ggcggggtcg
    11701 aggtggtctc cgtgggcgtg cgtgaggagg acgacgtccg ccttcgggag cgtcgcgtag
    11761 tcggcgagcc ttccgtacgg gtcgacgtgg aagaccttgc ctccgaagcg gaagaagagg
    11821 ctgccgtgcc cgacgaacgc gacctcgagc gggccggccg aggtggggat cacgtccttc
    11881 tcgaggccgg gcccagccgc gagcgcgacc ccggcgagcg gcgcgagcgc ggcgagaagg
    11941 agcagcccgg agacgaggtt cgtccgtccg tccatcggag cctcctcgcg gccgggtcgt
    12001 cgccggccgc gcgccgcgat cgtgatacgg atcggcccgc gcgtcgaggg tggggtcggg
    12061 aggccgcgcc tcgtcgggtc gaccgatcgc cggcgacgca cgcgcgttca gcgcgcgagt
    12121 cgccggcgca gcgcgcaggc caggatcccg atccagggga tcgagacgag ggcgttcccg
    12181 cgctgccaga ggccgacgtt cgcgcgagtg acgagccccc cgacgcccat catcacggcg
    12241 aacagcgccg tcatcgcgag gaagacgacg agaaggaaga gcctgagccg gccggcgtcg
    12301 ggcgcgcggc gcagccccca gagcaggaag agcggcgcca cctggaggcc gagtccaagg
    12361 ccgaacccgc cgtggcgcgg gtccggcatc gggaaggagc cgccgagcac catcgcgacc
    12421 ccccagaggg agaccgcgag ccccgagagg acggcccgca cgcggtcgct ccccaggcgg
    12481 cgaagcgcca cgccgaagcc gggaccggcg aggagcgcgg cgactcccgc ggcgacgatg
    12541 ccggagttga agatccaggg gtggggcgcc tccgccgcgc cgagctcgct cgcatagcgc
    12601 gtcacgtggc tgtagcccgg gtagagcgcg gagccgagga cgagggccag gaggtagagg
    12661 atcggcaccg cgatcccgaa gctcaggagg tggcgagggg agcgcatcga cgtcccttcc
    12721 tttcgtgtcg tcgcggcacg aaacggaagc cggggggagt cggttcgtgg gaggaggcgc
    12781 ccggagcggc ccctcaccgg gagtcgtggc tcggcggggc cggcgaaccc gacgtgacgg
    12841 gcgtcagccc ggccggcggg tgccctgcag cggatgccgc caccgaaggc ccggaaaccg
    12901 cgcgaagtcg cggtcggtcg tcacccattc gcatcccgac tcgaccgcaa gcgccgcgag
    12961 ccaggcgtcc gggacgaggt ttccccgcac gccgcactcc cggcagaggc gcgtgaagat
    13021 ttcccagtgc ctcgggcccg gcacgatctc gacggcattg gcgcggtcgc ggacctcgtg
    13081 gacgaaggcg agcgcgctct cgagcggcgt cggctcgcgg aagacgttcg ggtgcgtgac
    13141 gacgcgcagg aagccgctca ggacgaggtc ggagaccccg taggcggagt ccgccccgag
    13201 ggtcgcctcc agccacttcc ggtacttcgt gtggtcgggg gcgtcgcggc ggtgggcgtg
    13261 gacgaggacg ttaacgtcga acaggagcac gcggctcctc catgacgtcg aggagcgccg
    13321 cgctgtcgtc caggtcgacg ccgggccgga gcccctttcc gccgaaagtc gtcagccgca
    13381 ccggtgaggc cttcttcccg gtgcgtttgc ccgctaggga ctcccggagc gcgtcctcga
    13441 tcaccccggt cagggtcgtt cccgtctcga gggcccggcg acgcgcctgg tccagcaggg
    13501 gatcggggag gtggatcgtc gtcctcatgc atcaaagcat atcaagtagg catcacgatg
    13561 catcaacgga aatcgtggct cggcggcgtc ggcggacggt gcctcgggta cggcggcgaa
    13621 acgggcttcc tcagctcggg gatcggctcg tcgaggctca tccgctcgaa gcgctcggcg
    13681 cggacggaga gcgttccgtc cttcttctgg aggcgccctt cgacgacgag agcgggggag
    13741 ccgacgacga gggagcggtg ctcgcggaag aggtcggggc ggaggatggc ctggcacatc
    13801 ccggtctcgt cctcgaggga gaggaagacg aagcccttcg cggtgccggg gcgctgcctc
    13861 acgacgatga cgccggccgt cttcaccgtc gcgccgtcgg gggcgcgcat gaggtcggcc
    13921 gcggaaagga cgccttcgcg cttcagctgc gcgcggaagt gggcgatgag gtgcgggccg
    13981 gcggtcagct cggtggtctg gtagtcggag acggtctcct cgaagcggct catctcgggg
    14041 aggggcgagg
//
LOCUS       DAOTEZ010000060        13824 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3062_length_13824_cov_7.394727, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000060 DAOTEZ010000000
VERSION     DAOTEZ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13824
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3062_length_13824_cov_7.394727"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /gene="rocD"
                     /locus_tag="P5164_04840"
     CDS             complement(<1..1193)
                     /gene="rocD"
                     /locus_tag="P5164_04840"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HRY43255.1"
                     /translation="MPETATRSAALIEQESRYGAHNYHPLDVVCTKGEGIWLTDVEGK
                     RYMDFLAAYSAVNQGHNHPRIAEAMIRQARTLALTSRAFRNDQFPPLLEKLCKLTGFD
                     KALLMNSGAEAVETALKAMRKWAYDVKKVPYPEAEIVVAEGNFHGRTTTIVGFSTDPD
                     STSLFGPFTPGFRIVPYGDLAAVEAAITPKTAAIFVEPIQGEGGVIIPPDGYLKGLRE
                     IADRKKCLLVLDEIQSGLGRTGKLFAFEHEGIRPDGVTIGKALSGGFYPVSAFLASSA
                     VMDVFTPGIHGSTYGGNPLACAVASEALDVLVEERLVERAAELGVHLEARLRTMKTDK
                     VEAVRVRGLWAGVQLKLSAGPARPYCYQLKDRGMLCKDTHAQTIRLAPPLVITREEID
                     WAVDQL"
     gene            1374..2933
                     /locus_tag="P5164_04845"
     CDS             1374..2933
                     /locus_tag="P5164_04845"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRY43256.1"
                     /translation="MSGTGASLLPPPESIRLGAAFLLGAALLFVAARPARRHVARLVG
                     LWGASLALRLAAAAAARHLPSGEPTLSFLALLLQGIAFVGLAAVLLFDVLLPLAKVRA
                     PRIVRDVAVALSWVALLLWLFSVHKVDVTGIVATSAVVTAVIGFSLQDTLANVMGGIA
                     LQLEGAFAPGDWVKFGETSGKVAETGWRHTAIETRNGDVLLVPNAVLARTPLLLLGKA
                     KGRREPLERRWVFFGVDHRTPPAAVLETVREALTREPIPNVAAEPAPSVVLMEFRESW
                     ASYAVRYWLTDLLLDDPTDSEVRTRVAYALRRAGIPLAMPASANFVTVEEEGHRKRHE
                     KRDLEARLAALESVPLFSALTAEERERLAPGLVRAPFAPGEAMVVQGRQVHDLYILTK
                     GAGDVRVAVGDAPPRVVSRISAPDVFGEMGMLTGEPRRATVIAVGETECWRLGKERFR
                     EVLEGRPELAEELSHVLAARQVELATAREEISDESRRHRIETEHGSLRARIERFFGLG
                     ARGGAGERSGR"
     gene            2930..4348
                     /locus_tag="P5164_04850"
     CDS             2930..4348
                     /locus_tag="P5164_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRY43257.1"
                     /translation="MREQKLFLAGSWSDGEDELVVLSPWDGRPMASVARGGPATLERA
                     AEIAARSAGAIAALAPERRAAILEAARADLLARKEEVATAIVEEAGKPLTLARAEVDR
                     AADTLLASAHVARYPELVARDLGGFASGAGRIALTKRVPLGPVLAITPFNFPLNLVAH
                     KLGPAVAAGCPVVLKPASQTPGAALHLAASLQRAGLPDGALSVVPSRAADAERLATDS
                     RFALLTFTGSHEIGFGLRQKAWERRVALELGGNAAVIVEPDWDDLADAARRIASGAFA
                     WAGQSCISVQRVLVHRDVAPALRDLLVAAAEAIPVGDPSAPETLCGPMIDPANRDRVL
                     SWFSSARSAGGKLLSGGAADGSVVKPTLLEEVPEGEPLVSDELFGPGAALSAYATFDE
                     ALARANASRYGLQAGVFTRDIAKVRRAWETLEVGAVIQGDVPTWRSDPMPYGGVKGSG
                     VGREGPASAFLEMTEERMLVLR"
     gene            4429..4638
                     /locus_tag="P5164_04855"
     CDS             4429..4638
                     /locus_tag="P5164_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43258.1"
                     /translation="MSDEVEPKREDETPFVADGIAAGAYNEEGVDLTLIRMFLMLTPA
                     ERLDEASASANGVEELRALQAPASR"
     gene            4635..5126
                     /locus_tag="P5164_04860"
     CDS             4635..5126
                     /locus_tag="P5164_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43259.1"
                     /translation="MTFDFRRALTVLAAHEVEFIVVGGISAILRGAPVVTLDLDIVHR
                     RTPENVRRLLAALRSIDARYRHDSRGLVPEEVHLLGPGHNLLQTTCGPLDALGAIEDG
                     LSYDDLLSDSEAMRVGPDLSARVLSLRRLVELKAAAGRPKDLAALPTLRATLVELEKT
                     RGV"
     gene            complement(5196..6653)
                     /locus_tag="P5164_04865"
     CDS             complement(5196..6653)
                     /locus_tag="P5164_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRY43260.1"
                     /translation="MSLDALLPSPVRFLEGRLGRLRAPESLARYEAYFEERGRAVSEA
                     VDRAGTPKLLMFDRFGNRIDEVVFPPAYHELVRKAYEAGVVFRAVEEGDLAASYQIGY
                     VTSFYDAGLYCPHTVSLSTAIPLAKYGSAELRAKYLGRLLKRDDTVWQGATWMTEAKG
                     GSDLGAFVETVARSEGERWLLTGEKYFTSNVGAELAIAAARPEGAPAGVRGLELFLVP
                     RRRDDGALNFKVRRLKDKIGTRSVPTGEVELDGSEAYLLGERGQGVYLVLEALNVSRV
                     CNSIGAVALTQRALHEAWSFAEGRVAFGKPVAEHPLFLRQARERHETLLDSFALAWEA
                     VTLLSEVWRETPRYSDRYHLFRLVAHLAKYWTAEVAVQSAKWAMEAHGGAGILADYPV
                     ERLFREAIILGIWEGTPHRQVLDGMEVVEKKRVHVPLLDGIGARGGNAVALAMARAEV
                     DDWLSLPGEKKEAESERVFSRLAATVARELRRATL"
     gene            complement(6650..7075)
                     /locus_tag="P5164_04870"
     CDS             complement(6650..7075)
                     /locus_tag="P5164_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43261.1"
                     /translation="MALDVFHQVLAKLLEAAGGDPRQMVPFVEVLKQEKLFGSVEMIT
                     TRLQSEGWIADAPKRDHVFVTTWGVEELRRTNAAAATPKRAEPKNAAALRAAAEKARA
                     LAATLEELAAGTDGTAAQRKAKAKKALAAATEAVEEALG"
     gene            7158..7865
                     /gene="pxpB"
                     /locus_tag="P5164_04875"
     CDS             7158..7865
                     /gene="pxpB"
                     /locus_tag="P5164_04875"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HRY43262.1"
                     /translation="MTKDAPAGLPRLLPASDAALLVVLGDGPSEAATAAVLALLAELA
                     ARPPRGVLDLRPAYASLLVVFDPRTTTHAELARAVEPLLPPPGPPLAPAARCVEVPVC
                     YEGDCAPDLADVACGAGLSPEEAAELHAAAPYRVAFLGFSPGFAYLLGLPSPLATPRL
                     AAPRLVVPAGSVGIAGGQTGLYPRATPGGWRLVGRTPLALFSPSREPAALLAPGDAVR
                     FVAIPRREFDLLHRERP"
     gene            7862..8794
                     /locus_tag="P5164_04880"
     CDS             7862..8794
                     /locus_tag="P5164_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014465812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HRY43263.1"
                     /translation="MIRVLVPGLLTTVQDLGRSGLGAFGVPIGGAADAHALRLGNLLL
                     GNDEGAAALEATLVGPELLFEEAAVVALAGAPFGAQLDGVPLPVWEAVKVPAGGRLAM
                     GRAFSGTRAYVCVRGGLDVPSVLGSRATDVATGFGGLDGRPLRAGDRLSVGTARGEPR
                     GRRVDAAAARWSGPRRRLRVTRGPQGDWFREDVVEAFLSRPFRVSASSSRTGVRLEGK
                     PLAAPTRSLVTEGVVAGAVQVPPAGLPIVLLVEHPSTGGYPKIGSVISADLSALAQLR
                     PGEEVRFARVSFEEARRLFLDREARLGAPGVFLP"
     gene            8791..10017
                     /locus_tag="P5164_04885"
     CDS             8791..10017
                     /locus_tag="P5164_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY43264.1"
                     /translation="MRLAWLREASPEARRALLAAASGWMLDAMDVMLYAFALTAIRAE
                     FGLDGARAGLLASVTLVASAAGGIGAGLLADRFGRARVLVLSILAYSLFTGLTATSRT
                     VLELALWRTLVGLGLGAEWSAGSVLVAETWPAAHRGKAIGLMQSGWAVGYVLAAFLAA
                     AVLPTWGWRPLFVLGVLPGLLAVWVRRRVEEPAIWRERGAPAGRGTGLALLLKPPLLG
                     RAAAATSLATVLLFAYWGLFTWVPTFLAAPEAQGGAGLGLVRSTGFVVAMQAGAFLGY
                     TTFGFLSDRFGRRPVFLAFVLGAAVAVPAFGLLARSEAALLFLAPVVGYLGHGYFSVF
                     GVLLAELFPSSVRGAAQGFCYNAGRAVSALAPFSIGALADRHGLGSALALTSALYLAG
                     ALLMRLLPETRGRELE"
     gene            10014..10763
                     /locus_tag="P5164_04890"
     CDS             10014..10763
                     /locus_tag="P5164_04890"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HRY43265.1"
                     /translation="MRTIDLNADVGELPELAPVEEELLEVVTSVNVACGGHAGDEASM
                     ERVVRAALARGVAIGAHPSYPDRAGFGRHAMSLPGELLAETVAGQVASLLEVAARRGA
                     RLTHVKPHGALYNAAAKDVGLARAVAEGVARVAPDVVLVGLAGSEMLGAFAGAGFRVA
                     GEAFADRGYEPDGALTPRGRSGAMKETPEEAARQALSIACDGAVVAAGGARVPLSAQT
                     ICLHSDTPGAPAFARAIAERLRAAGVVLRAL"
     gene            complement(10873..11484)
                     /locus_tag="P5164_04895"
     CDS             complement(10873..11484)
                     /locus_tag="P5164_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43266.1"
                     /translation="MKKTFALVACALLVAGLAACNADKGPADAALKAADQAITAAAPE
                     AEKFIPEQLTAAKDALKAAQDQFAKGDYKAALAAATELGTKAQELATAAAAKKDELTK
                     AWTDLSGSLPQMVTAIQAKVEELGKAKKLPKGMDKAALTKAQEGLAGIQKSWEEASAA
                     FTGGNLVDAMAKAGGLKEKATEIMGALGLQAPAPAEAPAAPAS"
     gene            complement(11555..12580)
                     /locus_tag="P5164_04900"
     CDS             complement(11555..12580)
                     /locus_tag="P5164_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRY43267.1"
                     /translation="MSRSVLRAEIGEQPDVLARLLVREGPAIRRIARDLARRDLRFVL
                     VAARGTSDNAARFAQYALGLTCRLPVALAGPSLVTLYDSPPRLKGALVVGISQSGRSP
                     DVVETIASARREGVPTLAIVNDPDSPLAQAAADVIPLHAGVERSVAATKTYTAQLLAV
                     ALLAAGLSGSRKFAEGLGALPDAMADALGTEPAARRAAARLATAARLVVLSRGLNYPT
                     AHELALKLKELALLEAEALSGADFRHGPIALAGPKLPAIVVSPPGTATEEDLAGLADE
                     LRRRGSPVLAIGPHGRGSLPVPRLPELLSPIVAIVPGQLFAFHAARERGLDPDAPRGL
                     SKVTETR"
     gene            complement(12588..13001)
                     /locus_tag="P5164_04905"
     CDS             complement(12588..13001)
                     /locus_tag="P5164_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005003705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6524 family protein"
                     /protein_id="HRY43268.1"
                     /translation="MAGLGISVPGFLLRLLAAALLVFATYNPSGWSFLHWVRTSGGGP
                     LPLKVLAGIALAAGWVFCVRATLRSLGHVGTALAAAFFGVLIWLFVDLRGTDVEGSTL
                     AWIVLACLAATLAVGLSFSLVRRRVTGQIDDTDEE"
     gene            complement(13047..>13824)
                     /locus_tag="P5164_04910"
     CDS             complement(13047..>13824)
                     /locus_tag="P5164_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRY43269.1"
                     /translation="IANERHPIRFAQYVFAPKVREAGRKYPVVVLPHGGVHGDFGTYH
                     VHIVKEMLEQGWVVLAPEYRGSTGYGKGFYEAIDYGGLEVDDVIAGLDWAVSELPYVD
                     GSRAAIAGWSHGGLIALLAVMHHPEKFRAAYAGVPVTDLVTRLGYHEQSYEDLFAAKH
                     HVGKKVVEDVDEYRRRSPVFWASKLKTPLLVHATTNDRDVNVLEAENLIAALKAEGKS
                     FEHKIYTDAPAGHSFNRIDTRLAKESRKEIWAFLRKHLGR"
BASE COUNT         1610 a         5096 c         5139 g         1979 t
ORIGIN      
        1 agctggtcga cggcccagtc gatctcctcg cgcgtgatga cgaggggcgg cgcgaggcgg
       61 atcgtctgcg cgtgcgtgtc cttgcagagc atcccgcggt ccttcagctg gtagcagtag
      121 gggcgcgccg ggccggccga gagcttcagc tggacgccgg cccagaggcc gcggacgcgg
      181 accgcctcga ccttgtcggt cttcatcgtc cgaaggcgcg cctcgaggtg gacgccgagc
      241 tcggccgccc gctccacgag cctttcctcg acgaggacgt cgagggcctc ggaggcgacg
      301 gcacaggcga gcgggttgcc gccgtaggtg gagccgtgga tgccgggggt gaagacgtcc
      361 atcacggcgc tcgacgccag gaacgcgctg acggggtaga agccgccgga gagcgccttc
      421 ccgatcgtga cgccgtcggg gcggatcccc tcgtgctcga acgcgaagag ctttccggtc
      481 ctcccgaggc ccgactggat ctcgtcgagg acgaggaggc acttcttccg gtcggcgatc
      541 tcgcggagcc ccttgaggta cccgtcgggc gggatgatga cgcccccctc gccctggatc
      601 ggctcgacga agatcgcggc cgtcttcggg gtgatcgccg cctcgacggc ggcgaggtcg
      661 ccgtacggga cgatccggaa gcccggggtg aaggggccga agaggctcgt cgagtcgggg
      721 tcggtcgaga agccgacgat cgtcgtcgtc cggccgtgga agttcccctc ggcgacgacg
      781 atctcggcct cggggtaggg gaccttcttc acgtcgtagg cccacttgcg catcgccttg
      841 agcgcggtct cgaccgcctc ggcgccgctg ttcatcagga gcgccttgtc gaagccggtg
      901 agcttgcaga gcttctcgag gaggggcggg aactggtcgt tgcggaaggc gcggctcgtc
      961 agggcgagcg tgcgagcctg gcggatcatc gcctcggcga tccgcgggtg gttgtgcccc
     1021 tggttcacgg cggagtaggc ggcgaggaag tccatgtacc tcttcccctc cacgtccgtg
     1081 agccagatcc cctccccctt cgtgcagacg acgtcgagag ggtggtagtt gtgggcgccg
     1141 taccggctct cctgctcgat gagggcggcg gatcgggtgg cggtctcggg catggtcgtc
     1201 tccctccggc gggccctcgg gagcccgctt cgtcgtctct ctctgcggcc ggccgcggcc
     1261 gcgggtccgt ccgggcggga atcctaccgg aggcggggtg agccgggccg gctcccttcg
     1321 gaaagaggcg cgccgcctcc gccgccgcgt gccaaactcg aaggctcaga gcgatgtccg
     1381 gaaccggcgc ctccctcctc ccgccgccgg agtcgatccg gctcggggcc gccttcctcc
     1441 tcggggcggc gctcctcttc gtcgccgcgc ggccggcgcg gcggcacgtc gcgcgcctcg
     1501 tcggcctctg gggggcctcg ctcgccctcc gcctcgcggc cgccgccgcc gcgcggcacc
     1561 ttccgagcgg cgagccgacg ctctcgttcc tcgcgctcct cctgcagggg atcgcgttcg
     1621 tcggcctcgc ggccgtcctc ctcttcgacg tgctgctgcc gctcgcgaag gtgcgggccc
     1681 cccggatcgt ccgcgacgtc gccgtcgccc tctcctgggt cgccctcctc ctctggctct
     1741 tctccgtcca caaggtcgac gtgaccggga tcgtggccac ctcggcggtc gtcacggccg
     1801 tcatcggctt ctcgctccag gacacgctcg cgaacgtcat gggcggaatc gcgctccagc
     1861 tcgagggggc cttcgcgccg ggcgactggg tgaagttcgg ggagacctcc gggaaggtcg
     1921 cggagaccgg ctggcgccac accgcgatcg agacgcgcaa tggcgacgtc ctcctcgtcc
     1981 cgaatgcggt cctcgccagg acgccgctcc tcctcctcgg gaaggcgaaa gggaggcgcg
     2041 agccgctcga gcggcgctgg gtcttcttcg gcgtcgacca ccggacgccc cccgccgccg
     2101 tcctcgagac cgtccgcgag gcgctgacgc gcgagccgat cccgaacgtc gcggccgagc
     2161 cggcgccgag cgtcgtcctg atggagttcc gcgagagctg ggcctcctac gccgtccgct
     2221 actggctgac cgacctcctc ctcgacgacc cgaccgactc ggaggtgagg acgcgggtcg
     2281 cctacgccct ccggcgcgcg ggcatcccgc tcgcgatgcc cgcgagcgcg aacttcgtca
     2341 cggtggagga ggaggggcac cgcaagcgcc acgagaagcg ggacctcgag gcgcggcttg
     2401 cggccctcga gagcgtcccc ctcttctcgg cgctcaccgc cgaggagcgc gagcgcctcg
     2461 cgccgggcct cgtccgcgcg ccgttcgcgc cgggggaggc gatggtggtc cagggacggc
     2521 aggtgcacga cctctacatc ctgacgaagg gggcgggaga cgtgcgggtc gccgtcggcg
     2581 acgcgccgcc gcgcgtcgtc agccggatct cggcccccga cgtcttcggc gagatgggga
     2641 tgctgacggg cgagcctcgc cgggcgacgg tgatcgccgt cggcgagacg gagtgctggc
     2701 ggctcggcaa ggagaggttc cgcgaggtgc tcgaggggcg accggagctc gccgaggagc
     2761 tctcgcacgt cctggcggcg cggcaggtgg agctcgccac ggcgcgcgag gagatctccg
     2821 acgagtcgcg ccggcaccgg atcgagacgg agcatgggag cctccgcgcg aggatcgagc
     2881 gcttcttcgg cctcggcgcg cgcgggggag cgggggagag gagcgggaga tgagggagca
     2941 gaagctcttc ctggccggga gctggagcga cggggaggac gagctcgtcg tcctctcgcc
     3001 gtgggacggc cggccgatgg cgagcgtcgc gcgaggcggc cccgcgacgc tcgagcgggc
     3061 ggcggagatc gcggcccgct cggccggagc gatcgccgcg ctcgcgcccg agcggcgggc
     3121 cgcgatcctc gaggcggcgc gcgccgacct cctcgcgcgg aaggaggagg tcgccacggc
     3181 gatcgtcgag gaggcgggga agcctctgac gctcgcccgc gccgaggtcg accgtgcggc
     3241 cgacacgctc ctcgcctcgg cgcacgtcgc ccgctacccg gagctcgtcg cgcgcgacct
     3301 cggcggcttc gcgagcggcg cgggacggat cgccctgacg aagagggtcc cgctcgggcc
     3361 ggtcctcgcg atcacgccgt tcaacttccc gctcaacctc gtcgcccaca agctcgggcc
     3421 ggccgtcgcg gcgggctgcc ccgtcgtcct gaagcccgcc agccagacac cgggcgcggc
     3481 cctccacctg gccgcctcgc tccagcgcgc ggggcttccc gacggcgcgc tctcggtcgt
     3541 cccttcccgc gccgccgacg cggagcggct cgccaccgac tcccgcttcg ccctcctcac
     3601 gttcaccggc tcgcacgaga tcggcttcgg cctccggcag aaggcgtggg agagacgcgt
     3661 ggcgctcgag ctgggcggaa acgcggccgt gatcgtggag cccgactggg acgacctcgc
     3721 cgacgcggcg cggcggatcg cgagcggcgc cttcgcctgg gcgggccagt cgtgcatcag
     3781 cgtccagcgg gtcctcgtcc accgcgacgt cgcgccggcg ctccgcgacc tcctcgtcgc
     3841 ggcggcagag gcgatccccg tcggcgaccc ctccgcgccg gagacgctct gcggcccgat
     3901 gatcgacccg gcgaaccgcg accgcgtcct ctcctggttc tcctcggcac ggtccgccgg
     3961 cgggaagctc ctctcgggcg gcgcggccga cggctccgtc gtgaagccga cgctcctcga
     4021 ggaggtcccc gagggcgagc cgctcgtttc ggacgagctc ttcggcccgg gcgcggcgct
     4081 ctccgcctac gcgacgttcg acgaggcgct cgctcgcgcg aacgcctccc gctacggcct
     4141 ccaggcgggc gtcttcaccc gcgacatcgc gaaagtccgc cgcgcctggg agacgctcga
     4201 ggtgggcgcg gtcatccagg gcgacgtccc gacgtggcgg agcgacccga tgccttacgg
     4261 cggcgtgaag ggctccggcg tcgggcgcga aggccccgcg tcggccttcc tcgagatgac
     4321 cgaggagcgg atgctcgtcc tgcggtgagt ctgcttcgcc cccggacgat ccggccggcc
     4381 ccgccgggac gaggctttcg tgccgcgacg ttgactatga tgacgggcgt gtcggacgag
     4441 gtcgagccga agcgagagga cgagacgccc ttcgtcgcgg acgggatcgc agcaggggcc
     4501 tacaacgagg aaggggtcga cctgacgctc atccggatgt tcctgatgct gaccccggcc
     4561 gaaaggctcg acgaggcgag cgcgtcggcc aacggcgtcg aggagctgcg ggcgctgcag
     4621 gcccccgcgt cccgatgacg ttcgacttcc ggcgcgcact gaccgtcctc gctgcgcacg
     4681 aggtggagtt catcgtcgtc ggcgggatct cggcgatctt gcgaggagcg ccggtcgtaa
     4741 ccctcgacct cgacatcgtg caccggcgta ccccggagaa cgtccgtcgc ctgctcgccg
     4801 cgctccggtc gatcgacgcc cggtaccggc acgattcgcg aggcctcgtc cccgaggagg
     4861 tgcatctgct cggtccaggc cacaacctcc tgcagacgac ctgcggcccg ctcgacgcgc
     4921 tcggagcgat cgaggatgga ctctcctacg acgacctgct ctcggacagc gaggcgatgc
     4981 gggtcgggcc cgacctgtcg gcccgggtcc tttccctgcg ccgcctcgtc gagctgaagg
     5041 cggccgccgg caggccgaag gacctcgcgg ccctcccgac ccttcgggcg acactcgtag
     5101 agctcgagaa gacccggggc gtctgagccc cccgccgggc atcgcccgcc gggcgccacc
     5161 gggcttccct cgccgtcgtc cgctcccttc cttcctcaga gcgtcgcccg acgcagctcg
     5221 cgggcgacgg tcgcggcgag gcgggagaag acccgctccg actcggcttc cttcttctcg
     5281 cccgggagcg agagccagtc gtccacctcg gcccgcgcca tggcgagggc gacggcgttc
     5341 ccgccgcgcg ccccgatccc gtcgaggagc gggacgtgga ccctcttctt ctccacgacc
     5401 tccatgccgt cgaggacctg ccggtggggc gtcccctccc agatgccgag gatgatcgcc
     5461 tcgcggaaga gccgctcgac agggtagtcg gcgaggatgc cggcgccgcc gtgcgcctcc
     5521 atcgcccact tcgccgactg gaccgcgacc tcggccgtcc agtacttcgc gaggtgcgcg
     5581 acgaggcgga agaggtggta gcggtcggag tagcgcggcg tctcgcgcca gacctccgag
     5641 aggagagtca ccgcctccca cgcgagggcg aaggagtcga ggagcgtctc gtgccgctcg
     5701 cgcgcctggc gaaggaagag cggatgctcg gcgaccggct tcccgaacgc gacgcgcccc
     5761 tcggcgaagc tccacgcctc gtggagcgcc ctctgcgtca gcgcgacggc gccgatcgag
     5821 ttgcagacgc gcgagacgtt gagcgcctcg aggacgaggt agaccccctg gccgcgctct
     5881 ccgaggaggt acgcctcgct cccgtcgagc tccacctcgc cggtcgggac ggagcgggtg
     5941 ccgatcttgt ccttcaggcg gcggaccttg aagttgagcg cgccgtcgtc gcgccgccgc
     6001 gggacgagga agagctcgag gccgcggacg ccggcggggg ccccctcggg acgcgccgcg
     6061 gcgatcgcga gctcggcccc gacgttgctc gtgaagtact tctccccggt cagcagccag
     6121 cgctcgcctt cggaacgggc caccgtctcg acgaacgcgc cgaggtcgga gcctcccttc
     6181 gcctcggtca tccacgtcgc cccctgccag acggtgtcgt cccgcttcag gagcctcccg
     6241 aggtacttgg cccggagctc ggccgagccg tacttggcga gcgggatcgc cgtcgagagc
     6301 gagacggtgt gcgggcagta gagccccgcg tcgtagaagg aggtgacgta gccgatctgg
     6361 taggaggcgg cgaggtcccc ctcctcgacg gcccggaaga cgacgcccgc ctcgtacgcc
     6421 ttccggacga gctcgtggta ggcgggcggg aagacgacct cgtcgatccg gttgccgaag
     6481 cggtcgaaca tgagcagctt cggcgtcccg gcccggtcga ccgcctcgga aacggcccgg
     6541 ccgcgctcct cgaagtaggc ctcgtagcgg gcgagggact ccggggcgcg gaggcgcccg
     6601 agccgcccct cgaggaagcg gacgggcgag gggaggagcg cgtcgaggct catccgagag
     6661 cctcttccac cgcctccgtc gcggccgcga gggccttctt cgccttcgcc ttccgctgcg
     6721 cggcggttcc gtccgtgccc gcggccagct cctcgagcgt cgcggcgagc gcgcgggcct
     6781 tctcggccgc ggcgcggagc gcggcggcgt tcttcggctc cgcccgcttc ggcgtggcgg
     6841 ccgcggcgtt cgtgcgccgg agctcctcga cgccccaggt cgtgacgaag acgtggtcgc
     6901 gcttcggggc gtcggcgatc catccctccg actggaggcg cgtcgtgatc atctcgacgc
     6961 ttccgaagag cttctcctgc ttcaggacct cgacgaacgg gaccatctgc cgcgggtccc
     7021 cgccggccgc ctcgaggagc ttcgccagga cctggtgaaa gacgtcgaga gccatcgcag
     7081 cctccgcccg ttttccccga cggtaatccc gcgggagcgc cgcgtcgagg ggcggtccga
     7141 gcgtatcgtc ggggcgcgtg acgaaggacg ccccggccgg cctcccgcgc ctcctcccgg
     7201 cgagcgacgc cgcgctcctc gtcgtcctcg gggacgggcc ctcggaggcc gcgacggcag
     7261 cggtcctcgc gctcctcgcg gagctcgcgg cccggccccc gcgcggcgtc ctcgacctcc
     7321 ggcccgccta tgcctcgctc ctcgtcgtct tcgacccgcg gacgacgacg cacgcggagc
     7381 tcgcgcgcgc cgtcgagccg ctcctccctc cgcccggccc tccgctcgca cccgccgcgc
     7441 ggtgcgtcga ggtccccgtc tgctacgagg gcgactgcgc ccccgacctc gccgacgtcg
     7501 cgtgcggcgc ggggctctcg ccggaggagg ccgcggagct ccacgcggcg gcgccgtacc
     7561 gcgtcgcttt cctcggcttc tcccccggct tcgcctacct cctcggcctc ccgtcgccgc
     7621 tcgcgacgcc gcgcctcgcg gcgccccgcc tcgtcgtccc ggccgggagc gtcggcatcg
     7681 cgggggggca gacggggctc tacccgcgcg cgacgcccgg cggctggcgc ctcgtcggcc
     7741 ggacgccgct cgctctcttc tcgccgtctc gcgagcccgc cgcgctcctc gcgccgggag
     7801 acgccgtccg cttcgtcgcg atcccgcgcc gggagttcga cctcctccac cgggagcgcc
     7861 cgtgatccgg gtcctcgtcc ccggtctgct gacgacggtg caggacctcg gacggtcggg
     7921 gctcggggcc ttcggcgtcc cgatcggcgg cgcggccgac gcccacgccc tccgcctcgg
     7981 gaacctcctc ctcgggaacg acgagggggc ggccgcgctg gaggcgacgc tcgtcgggcc
     8041 cgagctcctc ttcgaggagg cggccgtcgt cgccctcgcc ggggcgccgt tcggggcgca
     8101 gctcgacggc gtcccgctcc cggtgtggga ggcggtgaag gtcccggccg gcggaaggct
     8161 cgcgatgggg cgagccttct ccgggacgcg cgcgtacgtc tgcgtgaggg gcgggctcga
     8221 cgtcccctcc gtcctcggga gccgcgcgac ggacgtggcg accggcttcg gcggcctcga
     8281 cggccggccg ctccgcgcgg gcgaccgcct ctccgtcggc acggcgcggg gagagccgag
     8341 ggggcggcgc gtcgacgccg ccgcggcgcg ctggagcggc ccccgccgcc ggctgcgcgt
     8401 gacgcgcgga ccacagggcg actggttcag ggaggacgtc gtcgaggcgt tcctctcgag
     8461 gccgttccgc gtctcggcct cctcgagccg gacgggagtc cgcctcgagg ggaagccgct
     8521 cgcggcgccg acccgctcgc tcgtcacgga gggagtcgtc gcgggggcgg tccaggtccc
     8581 gcccgcgggc ctcccgatcg tcctcctcgt cgagcaccct tcgacgggcg gatatccgaa
     8641 gatcggcagc gtgatctcgg ccgacctctc cgccctcgcc cagctccgcc cgggcgagga
     8701 ggtgcgcttc gcgcgcgtct ccttcgagga ggcgcgtcga ctcttcctcg accgcgaggc
     8761 gcgcctcggc gcgccggggg tcttcctccc gtgaggctcg cgtggcttcg cgaggcctcg
     8821 cccgaagccc gacgcgcgct gctcgccgcc gcctcgggct ggatgctcga cgcgatggac
     8881 gtcatgctct acgccttcgc cctgacggcg atccgcgcgg agttcgggct cgacggggcg
     8941 cgcgcggggc tcctcgcctc cgtgacgctc gtcgcctccg ccgccggcgg gatcggtgcg
     9001 ggcctcctcg cggaccgatt cgggcgggcg cgagtcctcg tcctctcgat cctggcctac
     9061 tccctcttca cgggcctcac ggcgacgtcg cgcaccgtcc tcgagctcgc cctctggcgg
     9121 acgctcgtcg gcctcggcct cggcgcggag tggtccgcgg gctcggtcct cgtcgcggag
     9181 acctggcccg cggcccaccg cggcaaggcg atcggcctga tgcagtcggg gtgggccgtc
     9241 ggctacgtcc ttgcggcctt cctcgcggcg gcggtcctcc cgacgtgggg ctggaggcct
     9301 ctgttcgtgc tcggcgtcct gccgggcctc ctcgccgtct gggtccgccg gcgcgtcgaa
     9361 gagcctgcga tctggcgcga gcggggggcg cccgccggga ggggaacggg cctcgcgctt
     9421 ctcctgaagc cgcctctcct cggccgggcg gcggcggcga cctcgctcgc gaccgtcctc
     9481 ctgttcgcct actgggggct cttcacctgg gtcccgacgt tcctcgccgc gcccgaggcg
     9541 cagggagggg cgggcctcgg cctcgtccgc tcgaccggct tcgtcgtcgc gatgcaggcg
     9601 ggggcgttcc tcggctacac gacgttcggc ttcctctccg accgcttcgg ccggcgcccc
     9661 gtcttcctcg cgttcgtcct cggggcggcc gtcgcggtcc ccgcgttcgg gctcctggcc
     9721 cgctcggagg cggcgctcct cttcctcgcg cccgtcgtcg gctacctcgg gcacggctac
     9781 ttctccgtct tcggcgtcct gctcgccgag ctcttcccgt cgtccgtccg cggcgccgcg
     9841 cagggcttct gctacaacgc gggccgggcg gtgagcgcgc tcgccccctt ctcgatcggc
     9901 gccctcgcgg accgccacgg cctcgggagc gccctcgccc tcacctcggc gctctacctc
     9961 gccggggcgc tcctgatgcg gctcctcccg gagacgcggg gacgggagct cgagtgagga
    10021 cgatcgacct gaacgccgac gtcggcgagc tcccggagct cgcgcccgtc gaggaggagc
    10081 tcctcgaggt cgtcacctcc gtgaacgtcg cctgcggcgg gcacgcggga gacgaggcct
    10141 cgatggaacg cgtcgtccgc gcggcgctcg cgcgcggcgt cgcgatcggc gcgcacccct
    10201 cgtatcccga ccgcgccggc ttcggccgcc acgcgatgag cctgccgggc gagctcctcg
    10261 ccgagacggt cgccgggcag gtcgcctcgc tcctcgaggt cgccgcccgc cgcggcgcgc
    10321 gcctcaccca cgtcaagccg cacggcgcgc tctacaacgc cgccgcgaag gacgtgggcc
    10381 tcgcccgcgc cgtcgccgag ggggtcgcac gcgtcgcgcc ggatgtcgtc ctcgtcggcc
    10441 tcgccggctc ggagatgctc ggggccttcg ccggggcggg cttccgggtc gccggggagg
    10501 cgttcgcgga ccggggctac gagccggacg gcgcgctgac gccccgcggc cggtcggggg
    10561 cgatgaagga gacgccggag gaggcggccc ggcaggcgct ctcgatcgcg tgcgacgggg
    10621 cggtcgtcgc ggctggcggc gcgcgcgtgc cgctgagcgc gcagacgatc tgcctccatt
    10681 cggacacgcc gggcgcaccc gccttcgccc gcgcgatcgc cgaacggctc cgggcggcgg
    10741 gggtcgtcct ccgggcgctc tgagaggccc gggagccttc gcgctctcgt aggggctgcc
    10801 gaagaagaag cggccccggc cgaagccggg gccgcgaaag cgccgcgaac ggcgcgagag
    10861 gacggtgcga gctcagctcg ccggggcggc cggggcctcg gccggggccg gagcctgcag
    10921 gccgagggcg cccatgatct ccgtcgcctt ctccttcagg ccccccgcct tcgccatcgc
    10981 gtcgacgagg ttcccgccgg tgaaggcggc cgaggcctcc tcccacgact tctggatgcc
    11041 ggcgagccct tcctgggcct tcgtaagggc ggccttgtcc atccccttcg ggagcttctt
    11101 cgccttgccg agctcctcga ccttggcctg gatcgccgtg accatctgcg ggagcgaccc
    11161 ggagagatcc gtccaggcct tcgtcagctc gtccttcttg gccgcggcgg cggtggcgag
    11221 ctcctgggcc ttcgtgccga gctcggtcgc ggcggcgagg gcggccttgt agtcgccctt
    11281 ggcgaactgg tcctgggcgg ccttgagggc gtccttggcg gcggtgagct gctccgggat
    11341 gaacttctcg gcctcggggg cggcggccgt gatcgcctgg tcggcggcct tgagggcggc
    11401 gtcggcgggg cccttgtcgg cgttgcaggc ggcgaggccg gccacgagca gggcgcacgc
    11461 gacgagggca aaggtcttct tcatcgagcg actccttcga gcattcgttt ccgcgactgt
    11521 gtgcggacca cccgatagat tactgccgtt ccggtcagcg ggtctcggtg actttcgaga
    11581 gcccgcgggg cgcgtccggg tcgaggcccc gttcacgagc ggcgtggaag gcgaagagct
    11641 gtccggggac gatcgccacg atcggggaga ggagctccgg gagccgcggg accggaaggg
    11701 agcccctccc gtgcgggccg atggccagga ccgggctccc gcgccggcgg agctcgtcgg
    11761 cgaggccggc gaggtcctct tcggtcgccg tcccgggggg cgagacgacg atcgccggga
    11821 gcttcggccc ggcgagcgcg atcgggccgt gccggaagtc ggctccggag agggcctcgg
    11881 cctcgaggag ggccagctcc ttcagcttca gcgcgagctc gtgcgcggtg gggtagttca
    11941 ggccgcgcga gaggacgacg aggcgggccg ccgtcgcgag gcgggcggcg gcccgtcggg
    12001 cggccggctc cgttccgaga gcgtccgcca tggcgtcggg gagggcgccg agtccctccg
    12061 cgaattttcg cgagcctgat aggccggcgg cgaggagcgc gacggccagg agctgggcgg
    12121 tgtaggtctt cgtggccgcc acgctccgct cgacgccggc gtggagcggg atcacgtcgg
    12181 ccgcggcctg cgcgagcggg ctgtcggggt cgttgacgat cgcgagcgtc ggcaccccct
    12241 cgcgccgcgc cgaggcgatc gtctcgacga cgtcgggcga gcgccccgac tgcgagatgc
    12301 cgacgacgag cgcccccttc aggcggggcg ggctgtcgta gagcgtcacg agcgaggggc
    12361 ccgcgagcgc cacggggagc cggcacgtca gcccgagcgc gtactgggcg aagcgggcgg
    12421 cgttgtcgga ggtgccgcgc gcggcgacga ggacgaaccg gaggtcgcgg cgggcgaggt
    12481 ctcgggcgat ccgccggatc gcgggcccct cgcggacgag gagccgggcc agcacgtcgg
    12541 gctgctcgcc gatctcggcg cgcaggacgg agcggctcat ccccgcctca ctcctcgtcc
    12601 gtgtcgtcga tctgcccggt gacgcgccgc cgcacgagag agaaggagag gccgacggcg
    12661 agcgtcgcgg cgaggcaggc gaggacgatc caggcgagcg tcgagccctc gacgtcggtc
    12721 ccccggaggt cgacgaagag ccagatcagg acgccgaaga acgccgccgc gagcgccgtc
    12781 ccgacgtgcc cgagggagcg gagcgtcgcc cgcacgcaga agacccaccc ggcggcgagc
    12841 gcgatgccgg cgaggacctt cagcgggagg ggcccgccgc cgctcgtccg gacccagtgg
    12901 aggaaggacc agcccgacgg gttgtaggtg gcgaagacga ggagggccgc ggccaggagc
    12961 cgcaggagga accccgggac cgagatgccg agtccggcca acgtgacctc ctgttcggaa
    13021 tcgtgccgct cagtctagtc gagccgctac cggccgaggt gcttgcggag gaacgcccag
    13081 atctccttgc gggactcctt cgcgaggcgc gtgtcgatcc ggttgaagga gtgcccggcg
    13141 ggggcgtcgg tgtagatctt gtgctcgaag ctcttccctt ccgccttgag cgcggcgatg
    13201 aggttctcgg cctcgaggac gttcacgtcg cggtcgttcg tcgtggcgtg gacgaggagc
    13261 ggcgtcttca gcttcgacgc ccagaagacg gggctccgcc gccggtactc gtcgacgtcc
    13321 tcgacgacct tcttcccgac gtggtgcttc gcggcgaaga ggtcctcgta gctctgctcg
    13381 tggtagccga ggcgggtgac gaggtccgtc accgggacgc ccgcgtaggc ggcgcggaac
    13441 ttctccgggt ggtgcatcac ggcgaggagg gcgatgaggc cgccgtggct ccagccggcg
    13501 atcgcggcgc gggagccgtc gacgtacggc agctcggaga cggcccagtc gaggccggcg
    13561 atcacgtcgt ccacctcgag gccgccgtag tcgatcgcct cgtagaagcc cttcccgtag
    13621 ccggtcgagc cgcggtactc gggggcgagg acgacccacc cctgctcgag catctccttc
    13681 acgatgtgga cgtggtacgt cccgaagtcg ccgtggacgc cgccgtgcgg caggacgacg
    13741 accgggtact tccgccccgc ctcgcggacc ttcggcgcga agacgtactg cgcgaagcgg
    13801 atcgggtgcc gctcgttcgc gatg
//
LOCUS       DAOTEZ010000061        13661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3116_length_13661_cov_5.127811, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000061 DAOTEZ010000000
VERSION     DAOTEZ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13661
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3116_length_13661_cov_5.127811"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..612)
                     /locus_tag="P5164_04915"
     CDS             complement(103..612)
                     /locus_tag="P5164_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43270.1"
                     /translation="MRADLDSNALLLVEDDVPFATCTAEFLSRRGHGVELAFDLVSAS
                     CALARRAWPAVVTDVDLSGPSGTEGLEVVSAAAALRRRPAIVVWSGCVSEGLRREALR
                     LGADAVLEKGSLWELEAELARQLAKRQPTGAVDGEDRMRDRPAAEGRSDCGKPKHPAE
                     SATVLGARQ"
     gene            767..1186
                     /locus_tag="P5164_04920"
     CDS             767..1186
                     /locus_tag="P5164_04920"
                     /inference="COORDINATES: protein motif:HMM:NF039593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HRY43271.1"
                     /translation="MRRKLSVLPALFFVLSLTPGLAAAHDGHAHGMEPILGTVAHIEG
                     DRLDVADPDGKTVSVTLTSATTYFRGTTEAKKADLKAGLRVAVETAHGKSGLEAKVVR
                     IGASEEVVWTCPMHPEVKSAGPGKCPKCGMFLEKKKA"
     gene            1215..1832
                     /locus_tag="P5164_04925"
     CDS             1215..1832
                     /locus_tag="P5164_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRY43272.1"
                     /translation="MKRSARVLLVAAAVAVVWGGYALATAVRRGFSARDEPSRAEEVV
                     ARAFRRWATPEAMRRVPNPVAASPEVLTRARRHFADHCAGCHGNDGSGRTPIGRRLYP
                     RAPDMRGAETQRLTDGELFSIIRNGIRLTGMPAWGDGTPKEDVGTWELVRFIRHLPRL
                     TPTEVAEMEALNPKGPAEYERARQIEAFLAGSGPTAPSSPERRMK"
     gene            1829..2728
                     /locus_tag="P5164_04930"
     CDS             1829..2728
                     /locus_tag="P5164_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43273.1"
                     /translation="MKKALGPASLLVLTLLASPALAQMGGGGMWNGPGDMMSNGSESL
                     IVPAGVRGDGKNGTFFVTDLWIRSQGNGTATLAFHPADAATADPAVTATVALSQPVTF
                     LPDLLSAQLGLTKAFGNVRISATVPIAAVARVYNQSGAGAYGLAFMGMPQRMSLTAMP
                     MTGTWPDRQFEMSMLGLLPQPGARVNATVVNTTATTVTGTLDVLDADGSAPAGTGPKS
                     WPFRIAGYSSTQVDDVLADVASRFPSGGAGLQLRVRLDRGAGGMVMAYAVVNDNLTND
                     GYVVMGSMMNSGLGMGSGSGMGM"
     gene            2816..6238
                     /locus_tag="P5164_04935"
     CDS             2816..6238
                     /locus_tag="P5164_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013111238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HRY43274.1"
                     /translation="MDIAAFHARWLGNEGGERANKDPFLNDLCDLLGVPRPDPATGDA
                     ERDLYVYEKPAVLLHAAGKPTIGKIDLYRHGRFLLEAKQGSNVTRTGRTRRGTNAWHG
                     MMSDALGQAKRYVETLDAPPPFLLVVDIGHCVDLYASFDGSRVYHPFPNALANRTYFA
                     DLEALLPTLHAVWEEPLSLDPLRRTTAVTREVAAHLAGLSKELAEAGHSPEAVATFLM
                     RAIFTMFAEDVGLLPNRLFTRTLYERWIPHPETFAPDVQALWTTMNRGGSFGFEGEIL
                     RFNGGLFAAPQALALTRRQLEMLHEAARCDWKNVEPSIFGTLLERALDPKERHALGAH
                     FTPRAYVERLVRATVEEPLRAEWDVVRTLVRTLVEDGTEAKIAEAEKAVRAFHEKLCA
                     TRVLDPACGTGNFLYVTLDLFKRLEAEVVRLLRDLRPETGATGFLDLHGVSVTPRQLL
                     GIEVKRWAKEIAELVLWIGWLQWHFRTHGDTAPDEPVLRDYGNIECRDAVLAWDRVQP
                     ETDPVTGKPVTRWDGETTKPSPVTGEPIPDETARVVVERYVNPRKATWPEAEYVVGNP
                     PFVGGWRMRSALGDGYVEALWSTRANVSEKADLVMYWWDTAATLVREGRLRRFGLITT
                     NSVTQVFQRRVLQEHLDGGVKLAFAIPDHPWVDSTDGAAVRIAMTVGTADVTEGRLLT
                     VVREDADVEGVAVPLFRETRGRIHADLSIGAALLSSTALRANDGVTSPGVQLYGDGFI
                     VSRELAESWGPGAMSAREAGVLRRYMNARDLMQTSRDVEVIDFFGFEEAGARRAHPAL
                     YQHVLLRVKPEREQNRRESIRRMWWRFGWERPVLRKQLQGLRRFVVTAETAKHRVFVF
                     ADAALLPDNMLTAIALEDACHLGVLSSRVHTRWALSAGGRLGYGNDPRYTKARCFDPF
                     PFPAASGAQTARIRSLGESLDAHRKRQQAIHSGLTITGMYNVLEKLRSGEALTAKEKV
                     IHEQGLVSVLKQIHDDLDAAVFEAYGWPPTLTDEEILERLVALNAERAEEERRGLVRW
                     LRPEFQNPGGAKAATQEALPATEDEEEEPATKAAAPLAWPKALPERIAAVRDLMARAP
                     SPRSLAAVASAFAKAKPKDVETVLESLASLGLLVSFDAPDGRHWQAAAAPRA"
     gene            6307..6858
                     /locus_tag="P5164_04940"
     CDS             6307..6858
                     /locus_tag="P5164_04940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRY43275.1"
                     /translation="MSTYKIAVLVGSLRRDSFNRKLARAVAKLAPPEFAFGEVRIDDL
                     PLYDQDDDGSPAASVRRLKDELAAAQGFLFVTPEYNRSIPGVLKNAIDHASRPYGQSS
                     WAGKPAGVMGASIGAIGTAMAQQHLRNVLAYLDVPTLGQPEVFIHAKDGLFDEAGDLA
                     AGSKAFLQGWMDRYVAWVKAHAA"
     gene            6881..7096
                     /locus_tag="P5164_04945"
     CDS             6881..7096
                     /locus_tag="P5164_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015610471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HRY43276.1"
                     /translation="MKRLQTYDAPGITVTFDPNVCRHSAVCLRTLPAVFDVGRRRWVA
                     PEAAAVSEVAAAIDACPSGALRYVLKD"
     gene            7369..7578
                     /locus_tag="P5164_04950"
     CDS             7369..7578
                     /locus_tag="P5164_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015257607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRY43277.1"
                     /translation="MRSFPAVVEKCPETGLFVGWVPGFPGAHSQGETLDELHRNLREV
                     LELLLEDGDPKLTTELVGVYTVQVA"
     gene            7581..7793
                     /locus_tag="P5164_04955"
                     /pseudo
     CDS             7581..7793
                     /locus_tag="P5164_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017315986.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            complement(7996..8223)
                     /locus_tag="P5164_04960"
     CDS             complement(7996..8223)
                     /locus_tag="P5164_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003400649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43278.1"
                     /translation="MADRPLEPDSPAARMRLALELSAVADAMRAQRFRRERPNASEDE
                     IEALMEAWRGERPGAEAGDSAGRSVQWPRRR"
     gene            complement(8378..8635)
                     /locus_tag="P5164_04965"
     CDS             complement(8378..8635)
                     /locus_tag="P5164_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43279.1"
                     /translation="MSAPVQVTLSPEEWKLVSALRAIPESPLKERLDTLFGEVLKFAV
                     DPKCSESQADGVPCTNTNMDCDSCRKVTDLIDTLRRRVSAG"
     gene            complement(8632..10062)
                     /locus_tag="P5164_04970"
     CDS             complement(8632..10062)
                     /locus_tag="P5164_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRY43280.1"
                     /translation="MTTSAFYGKFERHSHGEGLKGHSTHYCPGCGHGLAHKYLGEAID
                     ELGIQDKTVAVSPVGCSVFLYYYFDVGNTQAAHGRAPAVAIGHKLANPESIVISYQGD
                     GDLASIGLAEIVSTAQMGIPITVIFVNNAIYGMTGGQMAPTTLMGQRTATSPDGRVEF
                     MGQPMKMAELIAGLDGPVYVERVALFNPKQRNRAKKAIHKALQNQVEKRGFSFVEVLA
                     ECPTHLKATPEQAEKWVEEKMLPMFPLGVKKDETREPWWETKRPTFETDRVLAEIGAG
                     EEKAPRFCTSFPSHVAKSDISLKLAGSGGDGAQTAAMLIVHAGINEGFDATHIPAYGP
                     ESRGGTSYADVHVAADEVLSPASPNPDVLIAFNMPSLVKFGPDVRPGGTIIYDSSVIT
                     DYTPVMSGVKVVGVPMSEIAKDLGKLMVKNIVALGALQAATGIFPKETFLTAVRRALK
                     DKCAMIPLNEQAFEWGVKAASEVTVP"
     gene            complement(10059..11456)
                     /gene="vorB"
                     /locus_tag="P5164_04975"
     CDS             complement(10059..11456)
                     /gene="vorB"
                     /locus_tag="P5164_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HRY43281.1"
                     /translation="MPALKERPKPFVLPEFCKGCGRCISACPKHCISMGTEINPKTGL
                     TPIVLDLTNCNGCGLCIDACPEPYGLQQTPADADYELQDPAKLFGDRMTDAPEPVDVP
                     DQVVPLPRMEPLVLKGNYAAAIGALLAGCRHFFGYPITPSTEGAELMAKLMPHLDGTF
                     LQAVSEVATVNMMYGCGGAGMRSMTFTSSPGFSLMLEGISYMIGAEVPGVFINVMRGG
                     PGLGNIAPEQADIKLCCRGLGHGNTHAIVLTPSTPQEMLDLTMLAFELTFKYRNPVII
                     ASDGYLGQMTGKVSLPESMVKPGIPKWAVYGDLEHRRNLISSIYLSEVDLEKWNVVLN
                     AKYASMQVEQRADLYMCDDAEVILVACTTPARTAKGAVQVLRSRGIKAGLFRPITVWP
                     FPIEKLKTVLPNAKRIVVVEASNGQLEDELKIALANARIREYPEIEHVRHYGGVLPQM
                     EEVILKVAAGQEVTV"
     gene            complement(11610..12533)
                     /locus_tag="P5164_04980"
     CDS             complement(11610..12533)
                     /locus_tag="P5164_04980"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006541142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HRY43282.1"
                     /translation="MPVDAPLDPALDLAARAVEEAEAILVTAGAGIGVDSGLPDFRGP
                     EGFWRAYPAFRDLGLRFEQLASPHWFFRDPALAWGFYGHRLGLYRETVPHEGFGTLLR
                     WASGTRHGAFVFTSNVDGQFQKAGFPDGRVAECHGSIHHLQCLRGCGEPIRSAEGVVV
                     DVDPATCRAREPFPTCPGCRGLARPNILLFGDLGWDDARTSAQMETLGDWLDGVSGPL
                     VVLELGAGTSVPSVRTFSERAVQGTRRTLVRINPREPELGRGFGSPASDSLEHLLSTS
                     RHAARPSGISLPLGALEAIRAIDAQLAAGRR"
     gene            12614..>13661
                     /locus_tag="P5164_04985"
     CDS             12614..>13661
                     /locus_tag="P5164_04985"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HRY43283.1"
                     /translation="MRPFLTRALLLSGLLASTSCTQAPRVPTASPEAAPSGIDLAGMD
                     RSIAPGDDFFAWANGSWLRSAEIPADRSSWGSSGEMAERTAKRVADLIREAAASNAPA
                     GSEARKVGDTFATFLDEATIEARGLEPLRPELDRIDAVADATGLARALGGTLRADVDA
                     FNATDLYTPNVLGLWVAQDLDEPARYAPFLLQGGLGMPDRDYYLDPSPRMARIRESYL
                     RHAAKVLDLAGVADAGAVAERVVALETRIARNHATREESSDVAKGNNRWTRDDFPKRA
                     PGLDWSAFFDAAGLGGQQAFVVWQPEAVTGLAALVREVPLSTWKEYLSFRCLESAATY
                     LPKAFADEEFAFYGR"
BASE COUNT         2031 a         4717 c         4884 g         2029 t
ORIGIN      
        1 ggcgccggag cgtcgcctcg ttcacgagaa gggacatcac tgggtcgtag agaccggtca
       61 gagcgcgaga cctcagcgcc gggacgaacg gctcggccgc gctcactgcc gggctcccag
      121 caccgtggct gactccgctg ggtgtttcgg tttcccgcaa tcgctgcgtc cttccgcggc
      181 gggtcggtcc cgcatccggt cctcgccatc aaccgccccg gtcggctgcc gtttcgcaag
      241 ctgacgagcg agctccgcct cgagctccca caggctcccc ttctccagca cggcgtccgc
      301 accgagccgg agcgcctcgc gccggaggcc ctcgctcaca cacccgctcc agacgacgat
      361 ggccggacgc cgccgcagtg ccgccgcagc ggacacgacc tccaggccct cggtgccact
      421 cgggcccgag aggtcgacgt cggtgacgac ggccggccag gcccgccggg cgagggcgca
      481 cgacgcggag acgagatcga aagccagctc cacgccatgc ccgcgtctcg acaggaactc
      541 ggcggtacag gtggcgaagg ggacgtcgtc ttcgaccagg agcagggcat tcgaatcgag
      601 gtcggcgcgc atgtcctcat ggtgtgggcc gaaccttgga atccgatgag agctgaggcg
      661 tctcgccgct ctaatgctct tctaaggtcg gctccatatc ttcctcgtcc acgaggcggc
      721 gggcgtgggc ggctcgcccc accgccgtgc gacaggagga atcgacatgc gcaggaagct
      781 ctccgttctc ccggctctct tcttcgtcct gagcctgacg ccgggcctcg cggcagccca
      841 cgacggccac gctcacggga tggagccgat cctcggcacg gtggcgcaca tcgagggaga
      901 tcgcctcgac gttgccgacc ccgacgggaa gacggtgtcg gtcacgctca cctccgcgac
      961 cacctacttc cgtgggacga cggaggcgaa gaaggccgac ctgaaggccg ggctgcgcgt
     1021 cgccgtcgag acggcccacg ggaagtctgg tctcgaggcg aaagtggtcc gcatcggggc
     1081 gtcggaggag gtcgtctgga cctgcccgat gcatccggag gtgaagagcg cgggtccggg
     1141 caagtgtccg aagtgcggca tgttcctcga gaagaagaag gcgtgacgac ggggcggacg
     1201 gcgggagccg gtcggtgaag cggtcggctc gagtcctcct cgtggccgcg gccgtcgccg
     1261 tcgtctgggg cggatacgcg ctggcgacgg cggtccgtcg cggcttcagc gcgcgagacg
     1321 agccgtccag agccgaggag gtcgtggctc gggctttccg gcggtgggcg actccggagg
     1381 cgatgcgccg tgttccgaac cccgtcgccg cgagccccga ggtattgact cgggcgcgcc
     1441 gccacttcgc cgaccactgc gccggttgcc acggaaacga cgggagcgga cggacgccga
     1501 tcggacggcg cctctatccg cgcgcgcccg acatgcgcgg cgccgagacc cagcgcctca
     1561 ccgacggaga gctcttctcg atcatccgga acggaatccg cctcaccggc atgcctgcct
     1621 ggggcgacgg gacgccgaag gaagacgtcg gcacgtggga gctcgttcgg ttcatccgcc
     1681 accttccgag gctcacgccg acggaggtcg cggagatgga agccctcaac ccgaaaggcc
     1741 cggccgagta cgaaagggcc cgccagatcg aggcgttcct cgccggctcc ggcccgacgg
     1801 ctccgtcctc gcctgaaagg agaatgaaat gaagaaggcg ctcggcccgg ccagcctcct
     1861 cgtcctgacg ttgctcgcgt cccccgccct cgcccagatg ggcgggggag ggatgtggaa
     1921 cggccccggc gacatgatgt cgaatggctc cgaatccctc atcgtccccg cgggagtccg
     1981 cggcgacgga aagaacggga cgttcttcgt cacggacctc tggattcgct cccaggggaa
     2041 cgggaccgcg accctggcgt tccacccggc ggacgccgcc accgccgacc cggccgtgac
     2101 ggccactgtg gcgctctcgc agcccgtgac cttcctcccg gacctcctct ccgcgcagct
     2161 cggcctgacg aaagcgttcg ggaacgtccg gatcagcgcc acggtcccga tcgccgcggt
     2221 ggcccgcgtc tacaaccagt cgggggccgg agcgtacggt ctcgcgttca tggggatgcc
     2281 ccagcgcatg tcgctcacgg cgatgccgat gacgggcacc tggcccgacc gccagttcga
     2341 gatgtcgatg ctcggtctcc tgccgcagcc cggggcccgc gtgaacgcca ccgtcgtgaa
     2401 cacgaccgcg acgaccgtga ccgggacgct ggacgtcctc gacgccgacg ggagcgctcc
     2461 cgccggcacg ggaccgaaga gctggccctt ccggatcgcg ggctactcct cgacgcaggt
     2521 cgacgacgtc ctcgcggacg tcgcctcgcg cttcccgtcg ggcggagccg gcctccagct
     2581 ccgggtccgc ctcgaccgcg gcgcgggcgg aatggtcatg gcctacgccg tcgtgaacga
     2641 caacctgacg aacgacggct acgtcgtgat gggaagcatg atgaactcgg gcctggggat
     2701 gggatcgggc tccggcatgg ggatgtaggc gtcgaaaccg ttccccggga cgcggcttcg
     2761 cggggcgccg gccggcgccc ctctgctaac ctccccccgc gcctccgcgc ctcacatgga
     2821 catcgccgcc ttccacgccc gctggctcgg gaacgagggg ggagaacggg ccaacaagga
     2881 cccgttcctg aacgacctct gcgacctcct cggcgtgccg cgccccgatc cggcgacggg
     2941 cgacgcggag agggacctct acgtctacga gaagccggcc gtcctcctcc acgcggccgg
     3001 aaagccgacg atcgggaaga tcgacctcta ccgtcacggc cgcttcctcc tcgaggccaa
     3061 gcagggctcg aacgtcaccc gcacgggccg gacgcgccgc gggacgaacg cctggcacgg
     3121 catgatgtcc gatgccctcg ggcaggcgaa gcgctacgtc gagacgctcg acgccccgcc
     3181 cccgttcctc ctcgtcgtcg acatcgggca ctgcgtcgac ctctacgcgt cgttcgacgg
     3241 ctcgcgcgtc taccacccgt tcccgaacgc cctcgcgaac cgcacctact tcgccgacct
     3301 cgaagcgctc ctcccgacgc tccacgccgt ctgggaggag ccgctctccc tcgacccgct
     3361 ccggcggacg accgccgtca ctcgcgaggt cgccgcgcac ctcgccggcc tctcgaagga
     3421 gctggccgag gcggggcatt cccccgaggc ggtcgcgacg ttcctgatgc gggccatctt
     3481 cacgatgttc gcggaggacg tcggcctcct tccgaaccgc ctcttcacgc ggacgctcta
     3541 cgagcgctgg atcccgcacc ccgagacgtt cgcgcccgac gtccaggcgc tctggacgac
     3601 gatgaaccgg ggcgggtcgt tcggcttcga gggggagatc ctccgattca acggaggcct
     3661 cttcgccgcg ccgcaggccc tcgccctcac gcgccgccag ctcgagatgc tccacgaggc
     3721 ggcccgctgc gactggaaga acgtcgagcc ctccatcttc gggacgctcc tcgaaagggc
     3781 cctcgacccg aaggagcgcc acgccctcgg cgcccacttc acgccgcgcg cctacgtcga
     3841 acggctcgtc cgcgcgaccg tcgaggagcc gctccgcgcc gagtgggacg tcgtgaggac
     3901 gctcgtgagg accctcgtcg aggacgggac cgaggcgaag atcgccgagg ccgagaaggc
     3961 cgtccgcgcc ttccacgaga agctctgcgc cacgcgcgtc ctcgacccgg cctgcggcac
     4021 cggcaacttc ctctacgtca ccctcgacct cttcaagcgg ctcgaggccg aggtcgtgag
     4081 gctcctccgc gacctgcgcc ccgagacggg ggcgaccggc ttcctcgacc tccacggcgt
     4141 cagcgtcacc ccccgccagc tcctcggcat cgaggtgaag aggtgggcga aggagatcgc
     4201 cgagctcgtc ctctggatcg gctggctcca gtggcacttc cggacccacg gcgacaccgc
     4261 ccccgacgag cccgtcctcc gcgactacgg gaacatcgag tgccgcgacg ccgtcctcgc
     4321 ctgggaccgc gtccagcccg agaccgaccc cgtcaccggt aagccggtca cccgctggga
     4381 cggcgagacg acgaagccga gcccggtgac cggcgagccg atccccgacg agacggcgcg
     4441 tgtggttgtg gagaggtacg tgaacccgcg gaaggcgacg tggccggagg cggagtacgt
     4501 ggtggggaac ccgccattcg tgggcggctg gcggatgcgc tccgccctcg gtgacgggta
     4561 cgtcgaggcg ctctggtcga ctcgggcgaa cgtatctgag aaggccgacc tcgtcatgta
     4621 ctggtgggat accgctgcga cgctcgttcg agagggccgg cttcgtcgct tcggcctgat
     4681 cacgacgaac agcgtgacgc aggtctttca gcgcagggtg cttcaggagc acctcgacgg
     4741 aggtgtcaag ctcgcgttcg cgattcccga tcacccctgg gtcgattcga ccgacggcgc
     4801 ggcggtccga atcgcgatga cggtcgggac ggctgacgta acggagggaa ggttgctcac
     4861 ggtcgtccgg gaggacgctg acgtcgaggg agttgccgtg cctttgttcc gcgagacgcg
     4921 gggccggatt cacgcggacc tctccatcgg cgccgcgctc ctttcgtcga cggcacttcg
     4981 ggcaaacgat ggcgtgacga gccccggtgt tcagctctac ggcgatggtt tcatcgtctc
     5041 gcgagagctc gcggaaagct gggggcctgg ggcaatgtca gcacgtgagg caggcgtcct
     5101 gcggcgctac atgaacgctc gggacctgat gcagacctca cgcgacgtcg aggtcatcga
     5161 cttctttggc ttcgaagagg ccggagcgcg tagagcgcac ccggctctct atcagcacgt
     5221 cttgcttcga gtgaagccgg aacgcgaaca gaaccgacgc gagtcgatcc gtcgcatgtg
     5281 gtggcggttc gggtgggagc ggccggtcct acggaagcag cttcaggggc ttcgccgctt
     5341 cgtcgtgacc gcggagacgg cgaaacacag ggtcttcgtc ttcgccgatg cggccctgtt
     5401 gcccgacaac atgttgaccg cgatcgccct cgaagacgca tgccacctcg gtgtcctttc
     5461 gagccgcgta cacacgcggt gggcgctttc ggctggcggg aggctcggct atggcaacga
     5521 tccgcgctac acgaaggcgc gttgcttcga ccccttcccc ttccccgcgg cgagcggagc
     5581 ccagaccgct cgcatccgct ccctcggcga gtccctcgac gcccaccgca agcgccagca
     5641 ggcgatccat tcgggcctca cgatcaccgg gatgtacaac gtcctcgaga agctccggtc
     5701 gggcgaggcg ctgacggcga aggagaaggt gattcacgag caggggctcg tctcggtcct
     5761 gaagcagatc cacgacgacc tcgacgcggc ggtcttcgag gcctacggct ggccgccgac
     5821 gctgaccgac gaggagatcc tcgagcggct cgtcgcgctg aacgccgagc gggccgagga
     5881 ggagaggcgc gggctcgtcc gctggctgcg cccggagttc cagaacccgg gcggcgcgaa
     5941 ggcggcgacg caggaggcgc tcccggccac cgaagacgaa gaggaggagc cggccacgaa
     6001 ggccgcggcg ccgctcgcat ggccgaaggc gctccccgag cggatcgccg ccgtccgcga
     6061 cctgatggcc cgcgcgccgt ccccccgctc gctcgccgcc gtcgcgtcgg ccttcgcgaa
     6121 ggcgaagccg aaagacgtgg agaccgtcct cgagagcctc gcctccctcg gcctcctcgt
     6181 ctccttcgac gcccccgacg gccgccactg gcaggccgcc gccgcgcccc gcgcgtagcg
     6241 gcggcctttc ggtggtagcg tatgagacgt gttcgcaggt gagtagcctg cgacaggaga
     6301 gtcgacatga gcacgtacaa gatcgccgtc ctcgtcggga gccttcgccg cgactccttc
     6361 aaccgcaagc tggcccgcgc cgtggcgaag ctggcgccgc cggagttcgc attcggcgaa
     6421 gtccggatcg acgacctgcc cctgtacgac caggatgacg acgggagccc cgccgcgtcc
     6481 gtcaggcggc tgaaggacga gctcgcggcc gcccaggggt tcctcttcgt cacgcccgag
     6541 tacaacaggt ccattccggg cgtcctcaag aacgcgatcg accacgcctc gcggccctat
     6601 ggccagagct cctgggcggg gaagccggcc ggcgtcatgg gcgcctcgat cggcgccatc
     6661 ggcacggcga tggcgcagca gcaccttcgc aacgtcctcg cctacctgga cgtgccgacg
     6721 ctcggccagc ccgaggtctt catccacgcg aaggacgggc tgttcgacga ggcgggcgac
     6781 ctggccgcgg ggagcaaggc gttcctccag ggatggatgg accgctacgt cgcctgggtg
     6841 aaggcgcacg ccgcctgacc ggttttcggg aggtcgacgg atgaagagat tgcagaccta
     6901 cgacgcgccg gggatcacgg tgacgttcga cccgaacgtc tgccgccact ccgcggtctg
     6961 cctgcggacc ctgccggccg tcttcgacgt cggccgccgc cggtgggtgg cgccggaagc
     7021 cgcggcggtg tcggaagtgg ccgcggcgat cgacgcctgt ccgtcggggg ctctccggta
     7081 cgtcctgaag gattaggggg cgccggaggc gtaggagtag ggtgacggcc cgcgccgctc
     7141 gacaggccct cgtgcgagcc ggacttcgca ttctgccgat ggccacgatc gcccggtggc
     7201 aaaacggtgc ctcgccgagg agcatctctt ccacgacatc gccgagaacg tgaagcagct
     7261 cgggctcggg aagtagccgg cctcacgaac cgttcccgcc ggcgggcctg ccgccgccgc
     7321 gctcggcgca tggcggcgcc ggctggcgcc ggcgcgtaga atcgcgtcat gaggtcgttc
     7381 cctgccgtcg tcgaaaagtg ccccgagacg gggctcttcg tgggctgggt cccgggattc
     7441 cccggcgctc actcgcaggg cgagacgctc gacgagctgc atcggaacct ccgggaggtc
     7501 ctggagctcc tgctcgagga cggcgacccg aagctcacca ccgagctcgt cggcgtctac
     7561 acggtccagg tcgcctgacc gtgacgcccc ttcccgtgct gaagccgcgg gaggtcgaac
     7621 ggctcctcga gcgcctcggt ttcgtcgtcg tccggcagcg cggctctcat cgtcagtttc
     7681 ggcacgccga cggccgatag acaacggtcc cgtttcacgg cgggcgggac gtctctccgc
     7741 tccttcttcg gcagatcgcg cgcgacgtcg gcttgacgcc cgaggagctt ctcgcagcgg
     7801 atcgtcgaga gacgggcgcc ggtcaggaag cggttcaccg accgggacga ggaggggcgc
     7861 gagatcgcgc gcgtcccgcg gagcgtcctc cgcgggatcg acctgccgac gggctgaccg
     7921 gtccttacgg accgaagtcg gcgacggcgc ggtccagctc cgtggcgatc gcgcggaggg
     7981 ccgcctcgat cgggctcatc ggcggcgggg ccattgcacg gaccggcccg cggagtctcc
     8041 ggcctctgcg ccgggccgct cgcccctcca cgcctccatg agcgcctcga tctcgtcctc
     8101 gctcgcgttc gggcgctccc ggcggaagcg ctgcgcgcgc atggcgtcgg cgacggccga
     8161 aagctcgagc gcgaggcgca ttcgcgcggc gggggagtcg ggctcgagag gccggtctgc
     8221 cacgaggcga tgctacgagc ccgaaaacgg acccgcccgc cgttgcggga gggaaccggg
     8281 aggacccggc ccctcgccgc cccggcgggc gggagaatcg gtttccgggg gcgccgttcg
     8341 ccgccccccg gacccgggag acgacgagcg ccttcgccta gccggcggag acgcggcgcc
     8401 ggagggtgtc gatcaggtcg gtcaccttgc gacaggagtc gcagtccatg ttcgtgttcg
     8461 tgcaggggac gccgtccgcc tgcgactcgg agcacttcgg gtcgacggcg aacttcagga
     8521 cctctccgaa gagggtgtcg agacgctcct tcagggggct ctcgggaatg gcgcggaggg
     8581 cggagacgag cttccactcc tccggggaga gcgtcacctg cacgggcgcg ctcacgggac
     8641 ggtgacctcg gaagccgcct tgacgcccca ctcgaacgcc tgctcgttga gcgggatcat
     8701 ggcgcacttg tccttgaggg cgcggcggac ggcggtgagg aacgtctcct tcgggaagat
     8761 ccccgtggcc gcctggagcg cgccgagggc gacgatgttc ttgaccatga gcttgccgag
     8821 gtccttggcg atctccgaca tcgggacgcc gacgaccttc acgcccgaca tcaccggcgt
     8881 gtagtcggtg atgacggagc tgtcgtagat gatcgtcccg cccgggcgca cgtccggccc
     8941 gaacttgacg aggctcggca tgttgaacgc gatgaggacg tccgggttcg gggaggcggg
     9001 ggagaggacc tcgtcggcgg cgacgtggac gtcggcgtag gaggtgccgc cgcgcgactc
     9061 ggggccgtag gccgggatgt gcgtcgcgtc gaagccctcg ttgatgccgg cgtggacgat
     9121 gagcatcgcc gccgtctggg cgccgtcgcc gccggagccg gcgagcttca gcgagatgtc
     9181 gctcttcgcg acgtgagacg ggaacgacgt gcagaagcgg ggagccttct cctcgcccgc
     9241 gccgatctcg gcgaggacgc ggtcggtctc gaaggtcggc ctcttcgtct cccaccaggg
     9301 ctcgcgcgtc tcgtccttct tcacgccgag cgggaacatc gggagcatct tctcctcgac
     9361 ccacttctcg gcctgctcgg gcgtcgcctt caggtgcgtc gggcactcgg cgaggacctc
     9421 gacgaacgag aagccgcgct tctcgacctg gttctggagc gccttgtgga tcgccttctt
     9481 ggcgcggttc ctctgcttgg ggttgaagag ggcgacccgc tcgacgtaga cggggccgtc
     9541 gaggccggcg atcagctcgg ccatcttcat cggctggccc atgaactcga cccggccgtc
     9601 ggggctcgtg gccgtccgct ggcccatgag ggtcgtcggg gccatctggc cgcccgtcat
     9661 gccgtagatc gcgttgttga cgaagatgac ggtgatcggg atgcccatct gggccgtgga
     9721 gacgatctcg gcgaggccga tcgaggcgag gtcgccgtcg ccctggtagg agatgacgat
     9781 cgactcgggg ttcgcgagct tgtgcccgat cgcgacggcg ggggcgcggc cgtgggcggc
     9841 ctgcgtgttg ccgacgtcga agtagtagta gaggaagacg gagcatccga ccggggagac
     9901 ggcgacggtc ttgtcctgga tgccgagctc gtcgatcgcc tctccgaggt acttgtgcgc
     9961 gaggccgtgg ccgcagcccg ggcagtagtg ggtggagtgc cccttgagcc cctcgccgtg
    10021 cgagtggcgc tcgaacttgc cgtagaaggc ggacgtcgtc atacggtcac ctcctgcccc
    10081 gcggcgactt tcaagatgac ctcctccatc tgcggcagca cgccgccgta gtggcgcacg
    10141 tgctcgatct cggggtactc gcggatccgg gcgttcgcga gggcgatctt gagctcgtcc
    10201 tcgagctggc cgttcgaggc ctcgacgacg acgatccgct tcgcgttcgg gaggaccgtc
    10261 ttcagcttct cgatcgggaa gggccagacc gtgatcggtc ggaagagccc ggctttgatg
    10321 ccgcgcgagc ggaggacctg gacggctccc ttcgcggtcc gcgcgggggt ggtgcaggcg
    10381 acgaggatga cctcggcgtc gtcgcacatg tagaggtcgg cgcgctgctc gacctgcatc
    10441 gacgcgtact tggcgttgag aaccacgttc cacttctcga ggtcgacctc ggagaggtag
    10501 atcgaggaga tgaggttccg ccggtgctcg aggtcgccgt agacggccca cttcgggatg
    10561 cccggcttca ccatcgactc ggggagcgag accttgccgg tcatctggcc gaggtagccg
    10621 tcggaggcga tgatcacggg gttccggtac ttgaacgtca gctcgaacgc gagcatcgtc
    10681 aggtcgagca tctcctgggg cgtcgagggg gtcaggacga tggcgtgcgt gttgccgtgg
    10741 ccgaggccgc ggcagcagag cttgatgtcg gcctgctcgg gggcgatgtt gccgaggccg
    10801 ggcccgccgc gcatgacgtt gatgaagacg cccgggacct cggcgccgat catgtagctg
    10861 atcccctcga gcatcagcga gaagccgggc gaggaggtga acgtcatgga gcgcatcccc
    10921 gcgccgccgc agccgtacat catgttgacg gtcgcgacct cggagacggc ctgcaggaac
    10981 gtcccgtcga ggtggggcat gagcttcgcc atcagctcgg cgccctcggt cgagggggtg
    11041 atcgggtagc cgaagaagtg ccggcatccg gcgaggagcg cgccgatggc ggcggcgtag
    11101 ttccccttca ggacgagcgg ctccatcctc gggagcggga cgacctggtc ggggacgtcg
    11161 accggctcgg gggcgtcggt catccggtcg ccgaagagct tggcggggtc ctggagctcg
    11221 tagtcggcgt cggccggggt ctgctgaagg ccatagggct cggggcaggc gtcgatgcag
    11281 agtccgcacc cgttgcagtt cgtcaggtcg aggacgatcg gcgtgagccc ggtcttcggg
    11341 ttgatctcgg tccccatcga gatgcagtgc ttcgggcagg cgctgatgca gcgtccgcac
    11401 cccttgcaaa actcggggag gacgaacggt ttcggtcttt ccttcagggc gggcatgagg
    11461 cctcctccga cgaggagtct accggcccgg gcctgatgaa atttcaccat ttcccgggta
    11521 atcgtttcgg atcagagcat ggtatcagca cacgcgcaaa cgcgacagcc cgcgaagcgc
    11581 gcggccgcga gatgccggac ggcgggatct cagcggcgcc cggccgcgag ctgggcgtcg
    11641 atcgcgcgga tcgcctcgag ggcgccgagc gggagcgaga tccccgacgg gcgcgcggcg
    11701 tgcctcgagg tcgagaggag atgctcgagg gagtccgagg ccggcgaccc gaagccgcgg
    11761 ccgagctccg gctctcgagg gttgatcctc acgagggtcc gtcgcgtgcc ctgcaccgcc
    11821 cgctcggaga acgtccggac ggagggaacg ctcgtccccg cgccgagctc gaggacgacg
    11881 agcgggccgg agacgccgtc gagccagtcg ccgagcgtct ccatctgggc cgaggtccgc
    11941 gcgtcgtccc agccgaggtc gccgaagagg aggatgttcg ggcgggcgag gccgcggcag
    12001 ccgggacacg tcgggaacgg ctcgcgggct ctgcaggtgg ccgggtcgac gtcgacgacg
    12061 actccctcgg ccgaacggat cggctcgccg cagccgcgga ggcactggag atggtggatg
    12121 gagccgtggc actccgcgac gcgcccgtcg gggaagcccg ccttctggaa ctggccgtcg
    12181 acgttcgagg tgaagacgaa cgccccgtgc ctcgtccccg aggcccagcg caggagcgtg
    12241 ccgaagccct cgtgagggac cgtctcccgg tagaggccga ggcggtggcc gtagaagccc
    12301 caggcgagcg cggggtcccg gaagaaccag tgcgggctcg cgagctgctc gaagcggagg
    12361 ccgaggtcgc ggaacgcggg gtaggcgcgc cagaagccct cggggccgcg gaagtcgggg
    12421 aggccggagt cgacgccgat cccggcgccc gccgtgacga ggatcgcctc ggcctcctcg
    12481 acggcgcggg cggcgaggtc gagcgcgggg tcgagcgggg cgtcgacggg cacggggtga
    12541 ttgtagggaa ggggccgtcc cgcggccgag gttctccttg cgggacgaga agcgcctccc
    12601 tatcttcgtc gtcatgcgcc ccttccttac ccgggccctc ctcctctccg gcctcctcgc
    12661 gtcgacgtcg tgcacgcagg ctccccgcgt ccccacggcg tcgcccgagg ccgcgccttc
    12721 cggcatcgac ctcgccggga tggaccgttc gatcgcgccc ggagacgact tcttcgcgtg
    12781 ggcgaacggc tcctggctga ggagcgcgga gatccccgcc gaccggagct cctggggctc
    12841 gagcggggag atggccgagc ggacggcgaa gcgcgtcgcc gacctgatcc gggaggccgc
    12901 cgcctcgaac gctcccgcgg ggtcggaggc ccggaaggtc ggcgacacgt tcgcgacgtt
    12961 cctggacgag gcgacgatcg aggcccgggg gctcgagccc ctccggcccg agctggaccg
    13021 gatcgacgcc gtcgccgacg cgacggggct ggcgcgcgcc ctcggcggga cgctccgggc
    13081 ggacgtcgac gcgttcaacg ccacggacct ctacacaccg aacgtcctcg gcctctgggt
    13141 cgcgcaggac ctcgacgagc cggcccgcta cgccccgttc ctccttcaag gggggctcgg
    13201 gatgccggac cgcgactact acctcgaccc gtcgcctcgc atggcccgga tccgggagag
    13261 ctacctgcgg cacgcggcga aagtcctcga cctcgccggc gtcgcggacg cgggggcggt
    13321 cgcggagagg gtcgtggccc tcgagacgcg gatcgcccgg aaccacgcga cgcgagagga
    13381 gtcgtcggac gtcgcgaagg ggaacaaccg ctggacgcgg gacgatttcc cgaagcgcgc
    13441 gcccggcctc gactggtccg ccttcttcga cgcggccggc ctcggaggcc agcaggcgtt
    13501 cgtcgtgtgg cagccggagg cggtcacggg gctcgcggcg ctcgtccgcg aggtcccgct
    13561 gtcgacgtgg aaggaatacc tctccttccg ttgcctcgag tccgccgcga cgtaccttcc
    13621 gaaggccttc gccgacgagg agttcgcctt ctacggaagg g
//
LOCUS       DAOTEZ010000062        13576 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3144_length_13576_cov_5.811552, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000062 DAOTEZ010000000
VERSION     DAOTEZ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13576
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3144_length_13576_cov_5.811552"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..1192)
                     /locus_tag="P5164_04990"
     CDS             complement(182..1192)
                     /locus_tag="P5164_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006915606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HRY43284.1"
                     /translation="MDIAKNVFEVAVSDEPGRVKERRRFTKSQMLRFFANREPARVVI
                     EACGAAHALARELEWYGHEMVLLPPGQVRRYVLRNKTDRTDTKGLLEADRNEEIVPVP
                     VKTVAQQALVAVHRLRSGWMATRTARLNAIRGFLRELGVAVPVGATRVLPALTGAMEA
                     GRVPGTLEALLRTTAEEVRDLDRRIEEAESTLKRMSREMAAAVRLQTVPGIGLLTATA
                     RIGSVGEPGRFPSGRHLASFLGLVPRESSSGDRRRLGSITKRGDGYVRMLFTHGARSV
                     LRAASVKRQNDRLRTWALETQKRKGHNKATVALANKLTRIAWAVWLKDRPYEGRAPEE
                     TL"
     gene            complement(1572..2267)
                     /locus_tag="P5164_04995"
     CDS             complement(1572..2267)
                     /locus_tag="P5164_04995"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRY43285.1"
                     /translation="MTTFLAQMSFTWLALFPILNPPAMAPVFLGLTSAVTDRERNRLA
                     LLIGVYTALFLTVLLLVGGWILKILGISIPVVAIAGGILLFHSAWHMLNKDPRVSEEE
                     QAEIRSSMENKAFFPLTMPVTAGPGAMAVTLSLVPRGHILEASTLLAFLATACGILLA
                     GATVFVFYRFSGPVIRRMGDTGAATISQISAFVLLAIAVQIVWGGVSDLLVQTLEDPA
                     LAEGLRRFADRLR"
     gene            2434..3657
                     /locus_tag="P5164_05000"
     CDS             2434..3657
                     /locus_tag="P5164_05000"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HRY43286.1"
                     /translation="MKLFRSSLLIPALVLLVAMPATADMRGVPAPDDRPQATIEGTIT
                     SVNVPIAGGGPIVTLLNGLVAFDATGATVRYFNGLSATADTLAAGQRVLALLDPATAP
                     LRATTVVILSDRADVTFSGRVDAVDLGAGTLTVLGFTSKVTDRTVFGGPFDGVGSKGL
                     EDVRVGDMVFVEAKADAGALLATKVMKLGAPSDSVRRIHGTIASIGEAAWTITIPDGS
                     TLTVKVGPETKVTGAPKVGDTVDVLARPQSDGSLLAVAIFKSVPPPSVTLERFEGVVK
                     AIGATAWTIAPKGGGSDRTFAVDGETKVLGSPAVGDTVGVLAKKLDDGSWLAVVIAKA
                     MVSGPARTEVEFEGIVKSISPGNPTGVWSVGETKVVVTSRTVVKGEPKVGDTVKVEGL
                     KNPDGVVQASKIEKR"
     gene            complement(3744..5735)
                     /gene="uvrB"
                     /locus_tag="P5164_05005"
     CDS             complement(3744..5735)
                     /gene="uvrB"
                     /locus_tag="P5164_05005"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRY43287.1"
                     /translation="MSLFEVVSELTPQGDQPAAIEALVRGFREGREQQVLLGVTGSGK
                     TFTMAKVVEALDRPTLVLSHNKTLAAQLYQEFKRFFPRNRVEYFVSYYDYYQPEAYVP
                     QSDTFIEKETQINEEIDKLRIAATAALFERRDVLIVASVSCIYGLGSPESFGRMAIEL
                     KTGARYGLTWYLKRLVEAQYERTSLDLDRGTFRVRGDVLEIHPSYEDTGVRVEFFGDE
                     IEKVSRIDLLRGKVLETLGEFSLYPKSHYVTPEEVRQRALGSIEAELVTRVADLTAQK
                     KLLEAQRLEQRTRFDLEMMREIGYCAGVENYSRHLSGRTAGEPPPTLLDYFPADYLTI
                     VDESHQTIPQLRAMYHGDRSRKETLVEYGFRLPSALDNRPLRFEEWLERVGPRVYVSA
                     TPASYELEQAGGEVVEQVIRPTGLVDPRIEVRPVKGQVDDLLGRVRERAAKGERTLVT
                     TLTKRMAEDLTSYFLELGVRARYLHSDVETLERVQILGDLRRGVFDCLVGINLLREGL
                     DLPEVSLVAILDADKEGFLRSETALVQTAGRAARNLNGLVVFYADVVTDSMRRAMDET
                     ARRRAIQEAYNLEHGIVPETIVKSLDSPLLKMSNLDYGDSPALRAAGKDALRDDRSIE
                     EEIASLSKKMKEAARELEFETAAQLRDRIRELRAMQVFR"
     gene            complement(5732..6154)
                     /locus_tag="P5164_05010"
     CDS             complement(5732..6154)
                     /locus_tag="P5164_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43288.1"
                     /translation="MSSTPPPPISPGGPDAIREEKDARPGGVLKWGLVGCAGLSVLLI
                     IGMVLFLRKAPQLMESLLGATESQVVAAIAAEVPAADREAFRTEYAAFVETAKAGKAK
                     PDDIQALQKKIVAALEDRTVSREELKGLTEHLRSMPKK"
     gene            6220..8637
                     /locus_tag="P5164_05015"
     CDS             6220..8637
                     /locus_tag="P5164_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HRY43289.1"
                     /translation="MLGRRAAALLVLLLPVLLAAGLGVGAGWAASRLIRVPKVEQLAS
                     YRPDIVTEIRGADGSIVARFAIERRFLVTRDQLPEVLVQAVLAAEDARFYDHGGVDII
                     RIGGAALRDLATRRLAEGASTITQQLARSVFLTPQKSFARKLNEVFLTVEIEKRFSKD
                     QILTMYLNQVYLGHGNYGVEAASTWLFNHPARELTLTEAALLAGLIQRPEAFSPVRNP
                     AGAKSRRDLVLRRMAEVGYIDAATAAAAKAEPIVLSRSAREATIGPYFCEEVRQYLEK
                     TYGDRGLYRQGLRVESTVDPRIQAWAEEELRRGLRRHERRFAFRKPRNLVAEGIDPER
                     YRDPEWDEPDAPGRDPRAERAVVLSATRTGAELRVKDRRFTLPARAFRFIRAESPAKA
                     VKRGDVVLVERIEDEAGREETIVWQEPAVEGAVVVLENGTGAVRALVGGYDFSRSKFN
                     RAVQALRQVGSAFKPIVYMTAVEAGFTPADTVLDSPISITIDPRQPPWRPQNYTRSYG
                     GILTYQHALENSINVPAVRVDLLVGTRKVIETARRLGIRQNLLAYPSLALGSFEVTPM
                     EMTAAFSVFANQGLLFPPRLVERVRDAEGVLLEEDPPDPSEAASPTSSYVLLNMLTGV
                     TERGTAARARALGLKVAGKTGTTNDHTDAWFVGATPRHTIGVWVGHDAKKSLGAKSTG
                     GDTALPIWMGIVSRMKEAGLVKPSDDFDVPQGVVFVPYDLATGYRATPDCRRVVLGAF
                     ANGTQPTELCGERPHAVSTLPHYLQRAVYAPKRGEPAGAEVRVDDAPPARKGELPRSL
                     GESGPPG"
     gene            complement(8730..9173)
                     /gene="rplI"
                     /locus_tag="P5164_05020"
     CDS             complement(8730..9173)
                     /gene="rplI"
                     /locus_tag="P5164_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRY43290.1"
                     /translation="MKVILTDEVRGLGNRGEVVSVAAGYARNFLLPKELAYLATPGNV
                     KRLEQERKRYDVAQAKEKEFAATIAKAFEGLTVTIRKKAGENDALYGSVTSAELAEAL
                     EAKGITVDRRRIELDEPIKRLGAHTVHVRLHREVIAEMKVEVVAA"
     gene            complement(9190..10152)
                     /locus_tag="P5164_05025"
     CDS             complement(9190..10152)
                     /locus_tag="P5164_05025"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HRY43291.1"
                     /translation="MPFLAPPLAGLSAALGVVLPPLAPWLLPSAAARLARAGRELGTP
                     AAALPLAVAVLPFATFFGLVGGGVGSTLSAATALAFVVLPALGLLAGATAGRRREELA
                     WLAAALAGIGALGTVLAFTLAKGVDPGALLARRLDAARPELLGFYRNAGWSEETLAAT
                     GAAFDRLRDLVAWQLPGLLLSACVLHGALLVYAFGRRAGLDAAPLEAGSFATFRTPLA
                     AAAAFVPAGALAALADGAVARAATTVLIPLGVLFFLRGMAIIRALLDRGRVGLLIRAP
                     VYVLAVQMPIPVLVAIGGLLDEFLDFRGKFPRKEESGGETGRRE"
     gene            complement(10199..10462)
                     /gene="rpsR"
                     /locus_tag="P5164_05030"
     CDS             complement(10199..10462)
                     /gene="rpsR"
                     /locus_tag="P5164_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRY43292.1"
                     /translation="MAAPSGPAARRPAKKKFFVRRKRVCKFTVEKIGYIDFKDVKLLQ
                     SFVPERAKILPRRISGTSAFYQRKLAVAIKRARYLALLPYVAD"
     assembly_gap    10504..10603
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10604..11018)
                     /gene="rpsF"
                     /locus_tag="P5164_05035"
     CDS             complement(<10604..11018)
                     /gene="rpsF"
                     /locus_tag="P5164_05035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRY43293.1"
                     /translation="MTETQKRLYDLVFLIAPDKDEQGAGAVVEEFRKLLADLGAVIDK
                     DESMGRRRLAYQIKKRSEATYHNFLFRADAKAVAELQRKMKLSESILRFLTVRIDEEL
                     RHGQKVARRTKTRTPRVVPASPEFAPAPEAVAAPAP"
     gene            complement(11087..11704)
                     /gene="pth"
                     /locus_tag="P5164_05040"
     CDS             complement(11087..11704)
                     /gene="pth"
                     /locus_tag="P5164_05040"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRY43294.1"
                     /translation="MTELTLVVGLGNPGDRYVGTRHNVGWEVLDALVRRLASDPPRPT
                     RIDRLDCRALTGRVRIGATPVLLAKPQTYMNLSGESVKGLLLKHAVPPERMLVVSDDV
                     ALPVGRLRIRPSGSSGGQKGLQNVIDCLGSDGFPRLRIGIAGDHFRPGEDKADYVLER
                     FSKAERAALVPVLATACEAIETFVAEGLDVAMNRFNRSPEPVPAS"
     gene            complement(11707..12417)
                     /locus_tag="P5164_05045"
     CDS             complement(11707..12417)
                     /locus_tag="P5164_05045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRY43295.1"
                     /translation="MLQDNVVIEVEKRTETGKNACHRLRAKDLIPAVLYGGHSGAESL
                     PLSVARKDLLALFRKGLHQNAVFRVHVKGADEQPHVMVRDVMLSPLRRELVHLDFVRV
                     LLDRKLKVKVPVETVGIAAGVKLGGLLDVVTRELEVECLPADIPATIKADVTALDAGG
                     VLRVADLALGEKIQVLGDPDRVIAHVVTHKTEEAAAEAAAPATAEPEVVKKGKKEEEG
                     AAKPAAKAAPKAEKKEKK"
     gene            complement(12577..13533)
                     /locus_tag="P5164_05050"
     CDS             complement(12577..13533)
                     /locus_tag="P5164_05050"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRY43296.1"
                     /translation="MTLRVPYGELKVFGGRAHPSLTGEICRALGREAGRVTLYNFSDG
                     ENYCQIEENVRGADVFVVQPTCPPVNANLMELLIMLDAFKRSSASRVTAVLPYYGYAR
                     QDRKDKPRVPITAKLVADLLTSAGADRVLAVDLHAAQIQGYFDIPVDHLFASPVLLDA
                     IRDAKLDPLTVVSPDAGGVERARAFGKRLGVSLAIVDKRRKGKNEVEVMHVIGDVKGR
                     NVLIIDDIIDTAGTLINTVAALKEAGAERVFSACVHPVLSGPAIDRLNASSIERVFCS
                     NSLPIDDKLARCPILTPLSVGPLLGEAIHRIHEGTSVSSLFV"
BASE COUNT         1956 a         4766 c         4704 g         2050 t
ORIGIN      
        1 tgtggagcgg cacgccgtct ccgggccatg atgaggttcg cgcacggaga gccaatcgcg
       61 tagcacggct tcttcgagcc atcggggtta tcggctttcc tccgtgacgg tcggaccgtt
      121 ctcatcatct cgttggtttc gaagcgttcg ccggcgggat gcccttcccg tcttcacatt
      181 ctcagagggt ctcttcgggt gctctgccct cgtagggcct gtccttcagc cacaccgccc
      241 atgcgattcg cgtcagcttg ttcgccagtg ccaccgtcgc cttgttgtgg cccttcctct
      301 tctgcgtctc cagcgcccag gtcctgagcc ggtcgttctg cctcttcacc gacgcggccc
      361 gaagcaccga ccgcgctccg tgcgtgaaca gcatccgaac gtatccgtca ccccgcttgg
      421 tgatcgatcc cagccgtctg cggtcaccgc tggagctctc tctcggaacc aggcccagga
      481 acgacgccag gtgccggccc gacgggaatc ggcccggctc tccgacggag ccgatccgcg
      541 ccgtcgccgt caacagcccg atgcccggga ccgtctgcag ccgaaccgct gcagccatct
      601 cccgggacat ccgtttcagc gtgctctcgg cctcctcgat ccgacggtcc aggtcccgga
      661 cctcctccgc cgtcgttcgc agtaacgcct cgagtgttcc cggcacccga cctgcctcca
      721 tcgcgcccgt cagggccggc aggaccctcg tggcgcccac cggcaccgcc accccgagct
      781 cccgcaggaa gccccgaatc gcattcagcc gcgccgtgcg cgtcgccatc catcccgacc
      841 gcaacctgtg caccgccacc agcgcctgtt gcgccaccgt cttcaccggc accggcacga
      901 tctcctcgtt gcggtccgct tccagcagcc ccttcgtgtc cgtgcggtcc gtcttgttcc
      961 gcagcacgta gcgcctcacc tgccccggcg gcagcaggac catctcgtgc ccgtaccact
     1021 cgagttcccg cgccagcgcg tgcgccgccc cacacgcctc gatcaccact cgcgccggtt
     1081 cgcggttcgc gaagaacctc agcatctggc tcttcgtgaa ccgtcgccgc tccttcaccc
     1141 gcccgggctc gtccgagacg gccacctcga acacgttctt ggcgatgtcc atcgcgatcg
     1201 tcgtatgctt ttccacggac tcctcctttc tggtgctcct tccagagcat cagacgcctt
     1261 ggcactctga tgccgttcag gcgctccgga ggagtccatc gcatcgcgtc cagcggactc
     1321 gcggcgcttc gcgccgctcg ccgctgacgc tgcggttagc tgcgctaccg tggtgatgct
     1381 cgtcgcgcat cccgcgccat cgccctcggg caacgtcctg cacgcgtccg cgcccgtcac
     1441 ccccaggccc gccattcccc gcgcggtcgc ggccacgcct ttccgctttc ggcgtcgtgt
     1501 cggatccctt cgcctctggc acatccccgg catacgcacg gcggcgaaac ggagctgcac
     1561 ccgggtcgcc ttcagcgcag acgatctgcg aatcgcctga gcccttccgc gagggcggga
     1621 tcttcgagcg tctggacgag gaggtcgctc acgccgcccc agacgatctg gacggcgatc
     1681 gcgaggagga cgaaggcgct gatctggctg atcgtcgcgg cccccgtgtc gcccatcctc
     1741 cggatgaccg gcccgctgaa gcggtagaag acgaagaccg tcgctccggc gaggaggatg
     1801 ccgcaagccg tcgcgaggaa ggcgaggagc gtcgaggcct ccaggatgtg cccgcgcggg
     1861 acgaggctga gggtcacggc catcgccccc ggccccgccg tgaccggcat cgtgagcggg
     1921 aagaaggcct tgttctccat cgaggaacgg atctcggcct gctcctcctc ggagacccgc
     1981 gggtccttgt tcagcatgtg ccaggcggag tggaagagga ggatgccgcc ggcaatcgcg
     2041 acgaccggga tggagattcc gaggatcttc aggatccatc ccccgacgag gaggaggact
     2101 gtcaggaaga gggccgtgta gacgccgatc aggagggcga ggcggttccg ctcgcggtcc
     2161 gtcacggcgc tcgtcaagcc gaggaagacc ggagccatcg cgggggggtt gaggatgggg
     2221 aacaaagcca gccaggtgaa ggacatctgg gcgagaaagg tcgtcatgca gctcccgcgg
     2281 cgacccggcg tccgcggcgg ggatgctagc gcaggccgcc gaccgccgtc ccgccggacc
     2341 gaggcgttag gattccgggg ccgccgacac ttccgcggga gaccgaaccg attccggcgg
     2401 atggaaccct atccgaccca agcgaggttg ccgatgaagc tgttccgatc gtcgctcctg
     2461 atccccgccc tggtcctcct cgtcgcgatg cccgctaccg ccgacatgag gggcgtcccc
     2521 gcccccgacg accgcccgca ggcgacgatc gaggggacga tcacctccgt gaacgtcccg
     2581 atcgcgggcg gagggccgat cgtcacactt ctcaacggcc tcgtcgcgtt cgacgcgacc
     2641 ggcgcgacgg tccggtattt caacggactc tccgcgacgg ccgacaccct cgccgcgggc
     2701 cagcgcgtcc tcgccctcct cgacccggcc accgccccgc tccgggcaac gaccgtcgtc
     2761 atcctctccg acagggccga cgtcacgttc tccgggcgcg tcgacgcggt cgacctcggc
     2821 gccgggactc tcacggtcct cgggttcacg tcgaaggtga cggaccgaac cgtcttcggc
     2881 ggaccgttcg atggcgtcgg ctcgaagggc ctcgaggacg tccgcgtcgg cgacatggtc
     2941 ttcgtggagg cgaaggcgga cgccggcgcg ctcctcgcga cgaaggtcat gaagctcggc
     3001 gcgccttcgg actcggtccg ccggattcac gggacgatcg cctcgatcgg cgaggccgcg
     3061 tggacgatca cgatccccga cggctccacg ctcacggtga aggtcggacc ggagacgaag
     3121 gtcaccggcg ccccgaaggt cggcgacacc gtcgacgtcc tcgcgcgccc ccagtccgac
     3181 ggctccctcc tcgccgtcgc gatcttcaag tcggttccgc cgccttccgt cacgctcgag
     3241 cgattcgagg gggtcgtgaa ggcgatcggg gcgacggcct ggacgatcgc cccgaagggc
     3301 ggcggctccg atcggacgtt cgccgtcgac ggggagacga aggttctggg aagcccggcc
     3361 gtgggcgaca ccgtcggcgt cctcgcgaag aagctcgacg acggcagctg gctcgccgtc
     3421 gtcatcgcga aagcgatggt ctccggcccg gcgcgaaccg aggtcgagtt cgagggcatc
     3481 gtgaagagca tctcgccggg caacccgacc ggcgtctgga gcgtcggaga gacgaaggtc
     3541 gtcgtgacga gccggaccgt cgtgaagggc gagccgaagg tgggcgacac cgtcaaggtc
     3601 gaggggctga agaacccgga cggcgtcgtc caggcctcga agatcgagaa gcgctgacgg
     3661 tccgaaggcc ccctccgcgc cggcgcggag ggggcggggg cggcccggcc gttcccgact
     3721 tcgcggtgcg gaggccccgc ccgtcagcgg aagacctgca tggcccggag ctcgcggatc
     3781 cggtcgcgca gctgcgcggc cgtctcgaac tcgagctctc gggccgcctc cttcatcttc
     3841 ttcgagaggg aggcgatctc ctcctcgatc gagcggtcgt cccgcagggc gtccttaccg
     3901 gccgcccgaa gcgcgggaga gtcgccgtag tcgaggttcg acatcttgag gaggggcgag
     3961 tcgagcgact tgacgatcgt ctccgggacg atcccgtgct cgaggttgta ggcctcctgg
     4021 atcgcccggc ggcgcgcggt ctcgtccatc gcccgccgca tcgaatcggt cacgacgtcc
     4081 gcgtagaaga cgacgaggcc gttcaggttc cgggcggccc gccccgccgt ctggacgagc
     4141 gccgtctccg agcggaggaa cccctccttg tcggcgtcga ggatcgccac gagcgagacc
     4201 tccggcaggt cgagcccctc ccggaggagg ttgatcccga cgaggcagtc gaagacgccg
     4261 cgccgcaggt cgccgaggat ctggacccgc tccagcgtct cgacgtcgga gtggaggtag
     4321 cgggcccgca cgccgagctc gagaaagtag ctcgtgaggt cctcggccat cctcttcgtc
     4381 agcgtcgtga cgagggtccg ctcgcccttc gccgcccgct cgcggacgcg ccccaggagg
     4441 tcgtcgacct gtcccttcac cgggcggacc tcgatcctcg ggtcgacgag cccggtcggg
     4501 cggatcacct gctcgaccac ctcgcctccg gcctgctcca gctcgtacga ggcgggcgtc
     4561 gccgagacgt agacgcgcgg gccgacgcgc tcgagccact cctcgaaccg gagcggccgg
     4621 ttgtcgagcg cggacgggag gcggaagccg tactcgacga gcgtctcctt ccgggaccgg
     4681 tcgccgtggt acatcgcgcg gagctgcggg atcgtctggt ggctctcgtc gacgatcgtc
     4741 aggtagtcgg ccgggaagta gtcgaggagg gtcggcggcg gctcgcccgc ggtccgcccg
     4801 gagaggtgcc gcgagtagtt ctcgacgccg gcgcagtagc cgatctcccg catcatctcg
     4861 aggtcgaagc gtgtcctctg ctcgagccgc tgcgcctcca ggagcttctt ctgcgccgtc
     4921 aggtcggcca cccgcgtgac gagctccgcc tcgatggagc cgagggcccg ctgccgcacc
     4981 tcctccggcg tcacgtagtg cgacttcggg tagagcgaga actccccgag cgtctcgagg
     5041 accttcccgc ggaggaggtc gatccgggag accttctcga tctcgtcgcc gaagaactcg
     5101 acgcggaccc ccgtgtcctc gtacgagggg tgtatctcga ggacgtcccc gcggacgcgg
     5161 aacgtcccgc ggtcgaggtc gaggctcgtc cgctcgtact gcgcctcgac gagacgcttc
     5221 aggtaccagg tcagaccgta gcgcgcgccg gtcttcagct cgatcgccat tcgcccgaac
     5281 gactcagggc tcccgaggcc gtagatgcag gagaccgagg cgacgatgag cacgtcgcgc
     5341 cgctcgaaga gagcggccgt cgcggcgatc cggagcttgt cgatctcctc gttgatctgg
     5401 gtttccttct cgatgaacgt gtcggactgc gggacgtacg cctcgggctg gtagtagtcg
     5461 tagtaggaga cgaagtactc gacccggttc cgcgggaaga agcgcttgaa ctcctggtag
     5521 agctgcgcgg cgagcgtctt gttgtgggag aggacgagcg tcgggcggtc gagcgcctcg
     5581 acgaccttcg ccatcgtgaa cgtctttccc gacccggtga cgccgaggag gacctgctgc
     5641 tcgcgcccct cccggaagcc gcggacgagc gcctcgatcg ccgccggctg gtccccctgc
     5701 ggcgtcagct cggagacgac ctcgaagagg ctcacttctt cggcatggag cggaggtgct
     5761 ccgtcaggcc cttgagctcc tcccgggaga ccgtccggtc ctcgagcgcc gcgacgatct
     5821 tcttctgaag cgcctggatg tcgtccggct tcgccttccc cgccttcgcc gtctcgacga
     5881 aggccgcgta ttcggttcgg aaagcctccc ggtcggcagc cgggacctcg gccgcgatcg
     5941 cggcgacgac ctggctctcg gtggcgccga ggagcgactc catcagctga ggcgccttcc
     6001 gcaggaagag gaccatcccg atgatcagga ggaccgaaag gccggcgcag ccgacgaggc
     6061 cccacttcag gacgccgccc ggccgcgcgt ccttctcctc ccggatcgcg tccgggccgc
     6121 ccggggagat cggggggggc ggggtgctgc tcatcctctg cctcctcgcg gtgtctccgc
     6181 cccggggaac gggccggagg tgcatgtgag aatacctccg tgctcgggcg tcgcgccgcc
     6241 gcgctcctcg tcctcctcct cccggtcctc ctcgcggcgg ggctcggggt cggtgccggc
     6301 tgggcggcgt cgcggctcat ccgggtcccg aaggtcgagc agctcgcgag ctaccggccc
     6361 gacatcgtca ccgagatccg gggagccgac ggttcgatcg tcgcgcgctt cgccatcgag
     6421 cggaggttcc tcgtcacgcg ggaccagctc cccgaggtcc tcgtccaggc ggtcctcgcc
     6481 gccgaggacg cgcggttcta cgaccacggc ggcgtcgaca tcatccggat cggcggcgcc
     6541 gcgctccgcg acctcgcgac gcgccggctc gccgagggcg cctcgacgat cacgcagcag
     6601 ctcgcgcggt cggtcttcct gacgccgcag aagtctttcg cccggaagct caacgaggtc
     6661 ttcctgacgg tcgagatcga gaagcggttc tcgaaggacc agatcctgac gatgtacctc
     6721 aatcaggtct acctcggcca cggcaactac ggcgtcgagg cggcgtcgac ctggctcttc
     6781 aaccacccgg cgcgggagct cacgctgacg gaggcggcgc tcctggccgg gctcatccag
     6841 cggccggagg cgttctcgcc ggtccggaac cccgcgggag cgaaatcgcg ccgcgacctc
     6901 gtcctgcgcc ggatggccga ggtcggatac atcgacgcgg cgacggccgc cgccgcgaag
     6961 gcggagccga tcgtcctgtc ccgctccgcg cgcgaggcga cgatcggccc gtacttctgc
     7021 gaggaggtcc ggcagtacct cgagaagacc tacggcgacc gggggctcta ccggcagggc
     7081 ctgcgcgtcg aatcgaccgt cgacccccgc atccaggcct gggccgaaga ggagctccgg
     7141 cgcgggctcc ggcggcacga aaggcgcttc gcgttccgga agccccggaa cctcgtcgcc
     7201 gaagggatcg acccggagag gtaccgggac cccgagtggg acgagccgga cgcgccggga
     7261 cgggacccgc gcgcggaacg ggccgtcgtc ctttcggcga cgcggaccgg agcggagctc
     7321 cgcgtgaagg accgccgctt cacccttccc gctcgcgcgt tccgcttcat ccgtgccgag
     7381 agcccggcga aggcggtgaa gcgcggcgac gtcgtcctcg tggagcggat cgaggacgag
     7441 gcggggcgcg aggagacgat cgtctggcag gagccggccg tcgagggagc cgtcgtcgtc
     7501 ctggagaacg gcaccggcgc ggtccgcgcc ctcgtgggcg gctacgactt ctcgcggtcg
     7561 aagttcaacc gcgccgtcca ggcccttcgg caggtcggct cggcgttcaa gccgatcgtc
     7621 tacatgaccg ccgtcgaggc cggcttcacc ccggcggaca ccgtcctcga ctcgccgatc
     7681 tcgatcacga tcgacccgcg gcagccgccg tggcggccgc agaactacac ccggagctac
     7741 ggcgggattc tgacctacca gcatgcgctg gagaactcga tcaacgtccc cgcggtccgg
     7801 gtcgatctcc tcgtcgggac gcggaaggtg atcgagacgg cccgccggct cggcatccgg
     7861 cagaacctcc tcgcctaccc gtccctcgcc ctcggctcgt tcgaggtgac gccgatggag
     7921 atgacggcgg cgttctccgt attcgcgaac caggggctcc tcttcccccc gcggctcgtc
     7981 gagcgggtcc gcgacgccga gggagtcctc ctcgaggagg accctcccga cccgagcgag
     8041 gccgcctccc ccacctcctc ctacgtcctc ctgaacatgc tcacgggggt caccgagcgg
     8101 gggactgcgg cgcgggcgcg cgccctgggc ctgaaggtgg ccggaaagac cgggacgacc
     8161 aacgaccata ccgacgcgtg gttcgtcgga gcgacgccgc ggcacacgat cggcgtctgg
     8221 gtcggccacg acgcgaagaa gagcctcggc gcgaagtcga ccgggggcga cacggccctc
     8281 ccgatctgga tggggatcgt ctcccggatg aaggaggcgg gcctggtgaa accctccgac
     8341 gacttcgacg tcccccaggg cgtcgtcttc gtcccgtacg acctcgcgac cggctaccgg
     8401 gcgacgcccg actgtcgtcg cgtcgtcctc ggagcgttcg cgaacggcac gcagccgacc
     8461 gagctttgcg gcgagcggcc gcacgccgtg tcgacgcttc cccactacct ccagagggcg
     8521 gtctacgccc caaagcgggg agagccggcc ggcgccgagg tgcgggtcga cgacgcgccg
     8581 cccgcaagga aaggggagct gccgcgctcc ctcggggaaa gcggcccgcc gggctgaggg
     8641 gactccacgg tcccgcacga cgcccccgaa gacgcacgaa agccccgcgc tcgcggggct
     8701 ttcgtcttcg gagcggttcg cgggaacgtc taggccgcga cgacctcgac cttcatctcg
     8761 gcgatcacct cgcggtgcag acggacgtgg acggtgtgcg cgccgagccg cttgatcggc
     8821 tcgtcgagct cgatccggcg gcggtcgacg gtgatcccct tcgcctcgag cgcctcggcc
     8881 agctcggcgg aggtgacgga gccgtagagg gcgtcgttct ctcccgcctt cttgcggatg
     8941 gtgaccgtca gcccctcgaa ggccttcgcg atggtcgccg cgaactcctt ctccttcgcc
     9001 tgggccacgt cgtagcgctt ccgctcctgc tcgagccgct tcacgttgcc gggcgtggcg
     9061 agataggcga gctccttggg aaggaggaag ttgcgggcgt agccggccgc caccgagacg
     9121 acctcgcccc ggttgccgag gccgcgcacc tcgtcggtca ggatcacttt cacgtcggac
     9181 atgttccgct tactcccttc ttcccgtttc gccgccgctc tcttccttcc gggggaactt
     9241 cccgcggaag tcgaggaact cgtcgaggag cccgccgatg gccacgagga ccggtatcgg
     9301 catctggacg gcgagaacgt acaccggcgc tcggatcagg aggccgacac gccccctgtc
     9361 gagcagagcg cggattatcg ccatcccccg gaggaaaaac aagacgccca ggggaatcag
     9421 gaccgtcgtc gccgcacggg cgacggcccc gtcggccagg gcggccagcg cgcccgccgg
     9481 gacgaaagcc gcggcggcgg cgagaggtgt ccggaacgtc gcgaacgagc cggcctcgag
     9541 cggtgccgcg tccagccccg cgcgacggcc gaaggcgtag acgaggagcg ctccgtgcag
     9601 gacgcaggcc gagagaagga ggcccggcag ctgccacgcc acgaggtcgc ggagccggtc
     9661 gaatgcggcg cccgtcgccg cgagcgtctc ctcggaccac cccgcgttcc ggtagaagcc
     9721 gaggagctcc ggccgggcgg cgtcgagcct ccgggcgaga agcgcgcccg ggtcgacccc
     9781 tttcgcgagg gtgaacgcaa gcaccgtccc gagcgccccg atcccggcga gcgcggcggc
     9841 gagccaggcg agctcctcgc gcctccggcc ggcggtcgcc ccggcgagga gtccgagggc
     9901 gggaaggacg acgaaggcga gcgccgtcgc ggccgagagg gtcgatccga cgccgcctcc
     9961 gacgaggccg aagaacgtgg cgaaaggaag gacggcgact gcgagcggaa gggcggccgc
    10021 cggggtcccc agctcgcggc cggctcgcgc cagccgcgcc gcggccgacg gcaggagcca
    10081 cggggcgagc ggcggaagga ccacgccgag cgccgcagag agccccgcca ggggcggggc
    10141 cagaaacggc acgcccctcc ggcgcggagc cggaggggcg gagccggacg aaacctcgct
    10201 aatctgcgac gtagggcagg agcgccagat agcgcgcgcg cttgatcgcc acggcgagct
    10261 tgcgctggta gaaggccgag gtcccgctga tgcgacgcgg aaggatcttg gcccgctcgg
    10321 ggacgaagct ctggaggagc ttcacgtcct tgaagtcgat gtagccgatc ttctcgacgg
    10381 tgaacttgca gacgcgcttg cggcggacga agaacttctt cttcgcgggg cggcgggcgg
    10441 cgggaccgga cggagcagcc atcgatctct cctgactcag ctctcgggct gggccggcgc
    10501 ctcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgggggc cggggccgcg
    10621 acggcctcgg gggcgggagc gaactccggc gaagcgggga cgacgcgcgg cgtgcgcgtc
    10681 ttcgtccggc gcgccacctt ctggccgtgg cgcagctcct cgtcgatgcg gaccgtgagg
    10741 aagcggagga tgctctcgga gagcttcatc ttccgctgca gctcggccac ggccttcgcg
    10801 tcggcccgga acaggaagtt gtggtaggtc gcttcgctcc gcttcttgat ctggtaggcg
    10861 aggcggcggc ggcccatcga ctcgtccttg tcgatgacgg cgccgaggtc ggccaggagc
    10921 ttgcggaact cctcgacgac ggccccggct ccctgctcgt ccttgtcggg ggcgatcagg
    10981 aaaaccaggt cgtagagacg cttctgggtt tcggtcactt gtcctccttg cggatcgcgg
    11041 ggcgagcccg cgaggcgcgg cccttcggcc gagccaaggg ttggattcag ctcgccggaa
    11101 cgggctccgg cgagcggttg aaacgattca tggcgacgtc gagcccttcg gcgacgaagg
    11161 tctcgatcgc ctcgcaggcg gtcgcaagga ccgggacgag cgcggcccgt tcggccttcg
    11221 agaagcgctc gaggacgtag tcggctttgt cctccccggg ccggaagtgg tcccccgcga
    11281 tgccgatccg tagccgcggg aagccgtcgc tcccgaggca gtcgatcacg ttctggagcc
    11341 ccttctgccc cccggaggag cccgacgggc ggatcctcag ccggccgacc gggagggcga
    11401 cgtcgtcgga gacgacgagc atccgctccg gcgggacggc gtgcttgagg agaagccctt
    11461 tcacggattc gcccgagagg ttcatgtacg tctgcggctt cgcgaggagg accggcgtgg
    11521 cgccgatcct cacacgtccc gtcagggccc ggcagtcgag gcggtcgatg cgcgtcgggc
    11581 ggggcgggtc gctcgcgagc cgccggacga gcgcgtccag cacctcccag ccgacgttgt
    11641 gccgcgtccc gacgtagcgg tcgcccgggt tgccgagccc cacgacgagg gtgagctcgg
    11701 tcaagcctac ttcttctcct tcttctcggc cttgggcgcg gccttcgcgg ccggcttggc
    11761 ggcgccttcc tcttccttct tccccttctt cacgacctcg ggctcggccg tcgcgggggc
    11821 ggcggcctcg gcggcggcct cctcggtctt gtgcgtgacg acgtgcgcga tgacgcggtc
    11881 ggggtcgccg aggacctgga tcttctcgcc gagggcgagg tccgccacgc ggaggacgcc
    11941 gccggcgtcg agcgcggtga cgtcggcctt gatggtggcc gggatgtcgg ccgggaggca
    12001 ctcgacctcg agctcccgcg tgacgacgtc gaggaggccg ccgagcttga cgccggccgc
    12061 gatgccgacg gtctcgaccg ggaccttcac cttgagcttc cggtccagca ggacgcggac
    12121 gaagtcgagg tggacgagct cgcggcgaag cgggctcagc atcacgtcgc ggaccatgac
    12181 gtgcggctgc tcgtcggccc ccttgacgtg gacgcggaag acggcgttct ggtgcagccc
    12241 cttccggaag agggcgagga ggtccttccg ggcgacgctc agcgggagcg actcggcgcc
    12301 ggagtggcca ccgtagagga cggcggggat gaggtccttc gcgcggaggc ggtgacaggc
    12361 gttcttgccg gtctcggtcc gcttctcgac ctcgatgacg acgttgtcct ggagcatctc
    12421 gttcgtacct ttccccgggc ggacggtccg gcggaatccg ggcgtccggt ccggcgcaga
    12481 accgtggatt ctacacgacc gtcccggaaa cgcggcccgg cccggacgcc gcctccccga
    12541 tccggtggtc cgcccgctcc ccctgcctgc cggccgtcag acgaagagcg aggagaccga
    12601 cgtcccctcg tggatcctgt ggatcgcctc gccgaggagc gggcccaccg agagcggcgt
    12661 gaggatcggg cagcgggcga gcttgtcgtc gatcggcagc gagttcgagc agaagacgcg
    12721 ctcgatggag ctcgcgttca gccggtcgat cgcggggccc gacaggaccg ggtggacgca
    12781 ggccgagaag acccgctccg ctcccgcctc cttgagcgcc gccacggtgt tgatgagggt
    12841 cccggccgtg tcgatgatgt cgtcgatgat caggacgttc cggcccttca cgtcaccgat
    12901 gacgtgcatc acctcgacct cgttcttccc cttccgccgc ttgtcgacga tggcgaggga
    12961 gacgccgaga cgcttgccga acgcacgggc gcgctccacg cccccggcgt cgggcgagac
    13021 gacggtgagg gggtcgagct tggcgtcgcg gatcgcgtcg agcaggaccg gcgaggcgaa
    13081 gaggtggtcg accgggatgt cgaagtagcc ctggatctgc gcggcgtgca ggtcgaccgc
    13141 gaggacgcgg tcggctcccg ccgacgtcag gaggtcggcg acgagcttgg cggtgatcgg
    13201 gacgcgcggc ttgtccttcc ggtcctgccg ggcgtagccg tagtacggca ggaccgccgt
    13261 cacgcgcgac gccgaggagc gcttgaaggc gtcgagcatg atgaggagct ccatgaggtt
    13321 cgcgttcacc ggcgggcacg tcggctgaac gacgaagacg tcggcccccc ggacgttctc
    13381 ctcgatctgg cagtagttct cgccgtccga gaagttgtag agcgtcaccc gtcccgcctc
    13441 gcgtcccagg gcccggcaga tttcgccggt cagagacggg tgcgcacgcc ctccgaagac
    13501 cttcagctct ccgtacggaa ccctcagcgt catggctctc tcatcctggc tggggaggga
    13561 ggattcgaac ctccga
//
LOCUS       DAOTEZ010000063        13473 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3180_length_13473_cov_6.676107, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000063 DAOTEZ010000000
VERSION     DAOTEZ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13473
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3180_length_13473_cov_6.676107"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="P5164_05055"
     CDS             <1..398
                     /locus_tag="P5164_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY43297.1"
                     /translation="PYKARLRQIVPTADRQKGSVKVKIEILDRDARILPEMSCRVVFL
                     NPEAKVDAEAKPKVMVPAASVVEVGGKKGVLLVKESQALFRPLELGAAAGSQVEVVSG
                     VSGGEEIVAEAAGTEMKWRAEQKVRVKKD"
     gene            409..1155
                     /locus_tag="P5164_05060"
     CDS             409..1155
                     /locus_tag="P5164_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY43298.1"
                     /translation="MAEPLIKIESVVKQYTRDTQVLTVLDGLSLEVEKGDFVALMGPS
                     GSGKTTLLNLIAGIDRPTRGRIVVGDAEVSAMGEGALAKWRSENVGFIFQLYNLVPVL
                     TAFENVELPLLLTRLTKKERRKQTETALGVVGLSDRMDHYPRQLSGGQEQRVAIARAI
                     ATDPALLVADEPTGDLDAKSGEEILVLLERLHAEFGKTIVMVTHDPKAAARAHRLVHL
                     DKGVLKEDVRAKGEKGEIGIAAAAAIAAGR"
     gene            1160..2320
                     /locus_tag="P5164_05065"
     CDS             1160..2320
                     /locus_tag="P5164_05065"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY43299.1"
                     /translation="MKILPLVLKNLLRKKTRSALTIASIVLPLFVICLLGTLLRALEA
                     DPSGGKGMYRLIVRHKVSITNWLPGAYDPKIRTLPGVTEAVRMQWFGGAYIDQSAKNQ
                     FARFSVTDVDRFLRVYDEVSVVEGSEADWVGDRSGLLVGRQLMKRYGWRLGQKVSFKG
                     DIYPITLELTVRAVFAGPDESGVYFHHAAIEEALPRVKGYVGWYWLKTASVADTERIP
                     RQIDAMFENSSAPTRTETEKEFQSMWVSMLGNVKLLVGSITTIIGVVILLIAANTMAM
                     AARERVTEIAVLRVLGFTKEKVLGLVLGESFALSMIGGGLGLGLFVLLLPGFREGLVN
                     SPMGAFAAGIRLFPEVLALGFGVTFAVGLLAGLVPAVRSAQRPITEGLRQVA"
     gene            2317..3474
                     /locus_tag="P5164_05070"
     CDS             2317..3474
                     /locus_tag="P5164_05070"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY43300.1"
                     /translation="MKFVPYVLKSLFRKKTRSVLTILSIVLPFFVICVLGTLLATLDA
                     DPSGGKGMFRLVVRHKVSLANFIPEAYRERIAQLPGVEEVTILNWFGGTYKDNRPENM
                     FARFGCEPEKLVRIFDEVTIVEGTAAGWASDRKGALVGERLMKRYGWKIGDTFVLKGD
                     IFPMNLELTVRAVFRGSDETGVYFDRKAVEEALPFAKGQVGTFWIKAASAEAVQSLPR
                     QIDALFENSSFPTKTETEKEFQNGFVSMLGNVKAVVTGISTAIALVILLISANTMAMA
                     ARERVVEIAVLRTLGFGKPLILTLVLAESLLLSATGAFVGLGLFRLSFPALKEGLLSS
                     PMAGFAAGMQLFPEVLATGIAVTLLVGLVAGIVPAIRSARRSIPDGLRQVG"
     gene            3488..4666
                     /locus_tag="P5164_05075"
     CDS             3488..4666
                     /locus_tag="P5164_05075"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY43301.1"
                     /translation="MLSIPLKYNVRNLFVRKVTTSLTVLGIALVVAVFLCVMALGEGL
                     ATVFTASGSEKNVLVLRQNSQSELQSGISRDQAPLVKALPGIEKDADGKPLVSAELVV
                     VLNLDKRDGGSSNVTLRGVEEKGMALRPGMRITEGRAFVPGTSEVVVAESVARRFKGA
                     EVGQEIRFGAYRWKVVGHFDAGGTAPDSEIWTDTEGMLNTFQRGGYSSILARTTDRAA
                     RDQLVAALAGDARLALDGKVERAYYDEQTATAAPIRFLGVFVGVVMAIGACFGAMNTM
                     YAAVSARTREIATLRALGFSRLSILVSFVLESVALALLGGVAGCALAFLLVKLALSGV
                     TGTTNFATFAEVVFAFRLTPALLLTGIVFSLVMGFFGGLFPAGRAAFTKITAALRQVG
                     "
     gene            complement(4737..5111)
                     /locus_tag="P5164_05080"
     CDS             complement(4737..5111)
                     /locus_tag="P5164_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43302.1"
                     /translation="MLRIVGVVFALVGLGAGVFLLAGSFGLTPKSPGLSAWILFPACA
                     FGGLILAALGTRTAALPGLLKVAGGALLLLAVGSAVGLLLSAAGYAETRDGSGSLWYL
                     LGVAGLAGAAWLLAPERPEGKG"
     gene            complement(5187..5639)
                     /locus_tag="P5164_05085"
     CDS             complement(5187..5639)
                     /locus_tag="P5164_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43303.1"
                     /translation="MSRLVRLPLVLVLLAAAGCGGATATRNFATRAEAGAAVAEWVPA
                     GATELRLATEEGSGAFWLRFRLPKGGRTALEPELVRIPDTQVATLTFRAPKGADWWFR
                     GLVQVVPARETTVIAADVHSGRGAAVPLGTFVAFDRASDLVYAWQAGR"
     gene            complement(5653..6171)
                     /locus_tag="P5164_05090"
     CDS             complement(5653..6171)
                     /locus_tag="P5164_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43304.1"
                     /translation="MKRSLLSPLALPALLTAFVVATPSYAQEATVSSAEDKAQALAFV
                     QQLEADPLASGADNSRQWLTNFIVNAPDLFVSTCPELLGALNGQGKNGAPEIIGQLYF
                     GNAAFAIQNPDKASDREAMYAAGIESTLRLYASILKAKPRWRWPILDKLRSRAKDGTL
                     IEYVRENIGACQ"
     gene            complement(6246..6635)
                     /locus_tag="P5164_05095"
     CDS             complement(6246..6635)
                     /locus_tag="P5164_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43305.1"
                     /translation="MDPSFPSFVLAFSCGVVAFGVASVALAAVFVQGRFESSRLFLPA
                     AGVVASGLVAGFLVIVASSGGLPWGAVALPAALCIGLSARGLWIARGAPALAGYGRID
                     LWLFGTCLAGSALLAGLLLALRARVGG"
     gene            complement(6680..7159)
                     /locus_tag="P5164_05100"
     CDS             complement(6680..7159)
                     /locus_tag="P5164_05100"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRY43306.1"
                     /translation="MGEPVVSSELVQDFASRRRLAIAGVSRSGRKFGNLLLRELKARG
                     YEVVPVHPEAPDLQGLPCAKGLAELKGRVDGLVVVTPPSEAVKLVREAADAGIGRVWL
                     QQGAESDEAVRIAAERGVALVRGHCLLMFLPQAGGIHGFHRLLWRLLGKLPAGAARA"
     gene            complement(7209..7553)
                     /locus_tag="P5164_05105"
     CDS             complement(7209..7553)
                     /locus_tag="P5164_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43307.1"
                     /translation="MSETVELWGFVSDDVEAAREQAERLLGVSFRLHESESIGPYYFA
                     ELDEPESELTLRPNLDPDFDEDSGDPDDAFAEPDFPDFGVLLYVERKSEGGPGGGRLA
                     ALAVAAQLLLVE"
     gene            complement(7576..7896)
                     /locus_tag="P5164_05110"
     CDS             complement(7576..7896)
                     /locus_tag="P5164_05110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HRY43308.1"
                     /translation="MAADPITVLIRYQALPGKEDEALALLSELIATVVREEPDCLSIE
                     LLTYESDRGRILLYETWTSREAYLGPHFQTPHLKAFIEAAPASFAEAPDIAFWERHAV
                     AGRG"
     gene            complement(7908..8126)
                     /locus_tag="P5164_05115"
     CDS             complement(7908..8126)
                     /locus_tag="P5164_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43309.1"
                     /translation="MKAKVIVGLLLVALGVVALAYRSFTYTKKTHEAKVLGLELAVKE
                     KGTFEVPVWAGAGAVVAGALLLVAGRKG"
     gene            complement(8260..8661)
                     /locus_tag="P5164_05120"
     CDS             complement(8260..8661)
                     /locus_tag="P5164_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008949349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43310.1"
                     /translation="MSARVHGTPPVRATAEQSAEVFAALRAILSVHAEELKVTADSPK
                     GYSLNTPYSARWKKELFFGAVQVTKNYVSFHLFPVYMYPDLLEGATPELKARMQGKSC
                     FNFRAPDAALFAELAALTESGLARMKAEGAF"
     gene            complement(8800..9057)
                     /locus_tag="P5164_05125"
     CDS             complement(8800..9057)
                     /locus_tag="P5164_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HRY43311.1"
                     /translation="MSILWTILIGFVIGVIAKFLHPGKENLGLILTTALGIAGSLVAT
                     FLGRLVGFYKEGEAAGFLMAVVGAIVLLVVYTLVRGRSKQA"
     gene            complement(9106..9762)
                     /locus_tag="P5164_05130"
     CDS             complement(9106..9762)
                     /locus_tag="P5164_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HRY43312.1"
                     /translation="MNVLAAPPRPLTRGEEIANSVTHGIGLLASVVAFPFLVVAAARR
                     WGAPEVVGASVFAATLVALYLFSTLYHALRPSRAKQVLQKLDHTAIYLLIAGSYTPFT
                     LGVLRGPWGWTLFSLVWAIAGTGIALEFALGKKVHKVAVALYVVMGWLVVVAARPLLA
                     SVPWPGLAWLAAGGLLYTGGIAFYAAKRVRWAHTIWHLFVLAGSACHFVAIWGWSGRA
                     "
     gene            complement(9808..10350)
                     /locus_tag="P5164_05135"
     CDS             complement(9808..10350)
                     /locus_tag="P5164_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43313.1"
                     /translation="MILALVLLALAGPPSASATPSSAPPRETGAPAFGPEWRKLLGEW
                     KGGGSGAPGAGGGGSSFRLELDGHVLVRRNVADYPAAEGRPAAHHEDLLVVSPGATPS
                     TGSAVYFDNEGHVIRYDASWSADGAVLTLVSDAAAPGPRFRLTYACPAPDEMTVTFEV
                     APPGGAFRRYLGGDLRRVAR"
     gene            complement(10464..12788)
                     /locus_tag="P5164_05140"
     CDS             complement(10464..12788)
                     /locus_tag="P5164_05140"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRY43314.1"
                     /translation="MAVLGVAVAGLLYALFLARQVLAADTGTPRMQEVAAAVREGSNA
                     YLAAQFKRIGPLIIVLTILLFATYTGSENAFRWGRAGAFLVGALFSWAVGFVGMRLAT
                     AGNLRVAAAARSSYGEAMQLGYRTGTVTGMLTDGLGLLGGTIIFLIFSEKAYEALLGF
                     GFGGTLLALFMRVGGGIYTKAADVGADLVGKIEKDIPEDDPRNAATIADNVGDNVGDC
                     AGMAADIFESYEVTIVAAMILGMATFGHKGVIFPLLVRGIGVLGSIISTYTVKAGPND
                     TSDTALKSVHRGFWIGSLISIGGFFALGFSYLRFDAAYLEANPMAKAGFPNGDPTALG
                     GLLNWGIPGLDLRPALTCLIGVFLAIALNKVTSYFTHTGHAPVKGLARSCQTGHATNI
                     IQGFAVGYESTVMAIIVIAGAIGLSVLCYAGTPPLFIAYGVAMAGIGMLTLTGNTISM
                     DVFGPVADNANGIGEMAYDVKEMDAKAPGSAKRARQILADLDAVGNTTKAETKGIAIG
                     SAVIAAVSLFSSFIAVIAVGSEEKINEMTTAQYLAEAGRLTVADPKVFIGFLLGGAVP
                     FLFSSMLIRAVGRAAFYIVNECRVQFRDAAIWAGTKKPDYGRVVAICTTTAQKELIGP
                     GFLAIMTPILVGFLLGPYALGGFLAGMILVGQLLAVFMANAGGAWDNAKKLIEDGIYG
                     GKGSEAHKAAVTGDTVGDPLKDTAGPAINPLIKVMNMVSLLTLGLVLQHNLLSPAGGS
                     RVTGVVVVLVALAAIGWAVWQSKREDEGTEGLEK"
     gene            complement(13160..>13473)
                     /locus_tag="P5164_05145"
     CDS             complement(13160..>13473)
                     /locus_tag="P5164_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding protein"
                     /protein_id="HRY43315.1"
                     /translation="YRNYSAETSILRWPKVRAGEEKHIFPFQERCDAMFNSALAYETA
                     VLRNFAYRYLLEVPPDSPAQAKGYQLRRFLELFVPVWPDDVPATSILREFIGGSGFSY
                     K"
BASE COUNT         2084 a         4653 c         4833 g         1903 t
ORIGIN      
        1 agccctacaa ggcgcgcctc cgccagatcg tcccgacggc cgaccggcag aagggatcgg
       61 tcaaggtcaa gatcgagatc ctcgacaggg acgcgcggat cctccccgag atgtcgtgcc
      121 gcgtcgtctt cctgaacccc gaggcgaagg tcgacgcgga ggcgaagccg aaggtgatgg
      181 tcccggcggc ctcggtcgtc gaggtgggcg ggaagaaggg ggtcctcctc gtgaaggagt
      241 cgcaggccct cttccgcccg ctcgagctcg gcgcggcggc ggggagccag gtggaggtcg
      301 tctcgggcgt ctccggcggc gaggagatcg tcgccgaggc ggccggaacg gagatgaagt
      361 ggcgggcgga gcagaaggtc cgcgtgaaga aggactgacg aggaagcgat ggccgaaccc
      421 ctcatcaaga tcgagagcgt cgtcaagcag tacacgcgcg acacgcaggt cctcaccgtc
      481 ctcgacggcc tctccctcga ggtcgagaag ggggatttcg tcgccctgat gggcccttcg
      541 ggctcgggga agacgaccct cctcaacctg atcgccggca tcgaccggcc gacccgcggg
      601 aggatcgtcg tcggagacgc cgaggtctcc gcgatgggcg aaggcgccct cgcgaagtgg
      661 cggagcgaga acgtcgggtt catcttccag ctctacaatc tcgtccccgt cctgacggcg
      721 ttcgagaacg tcgagctgcc gctcctcctg acgcgcctga cgaagaagga gcggaggaag
      781 cagaccgaga cggccctcgg cgtcgtcggc ctctccgacc ggatggacca ctatccccgc
      841 cagctctccg gcggccagga gcagcgtgtc gcgatcgccc gggccatcgc gacggacccg
      901 gcgctcctcg tcgccgacga gccgacggga gacctcgacg cgaagtcggg agaggagatc
      961 ctcgtcctcc tcgagcggct ccacgccgag ttcggcaaga cgatcgtcat ggtgacgcac
     1021 gacccgaagg ccgccgcgcg cgcccaccgg ctcgtccacc tcgacaaggg ggtcctcaag
     1081 gaggacgtcc gggcgaaggg cgagaaggga gagatcggga tcgccgcggc cgcggcgatc
     1141 gccgccggca ggtagtccga tgaagatcct cccgctcgtc ctgaagaacc tcctgaggaa
     1201 gaagacgcgc tcggcgctga cgatcgcctc gatcgtcctg ccgctcttcg tcatctgcct
     1261 cctcgggacg ctccttcggg ccctcgaggc cgacccctcg ggcgggaagg ggatgtaccg
     1321 gctcatcgtc cggcacaagg tctcgatcac gaactggctc ccgggcgcct acgacccgaa
     1381 gatccggacg cttcccggcg tgaccgaggc cgtccggatg cagtggttcg gaggggcgta
     1441 catcgaccag tcggcgaaga accagttcgc gcggttctcc gtgacggacg tcgaccgctt
     1501 cctccgcgtc tatgacgagg tctccgtcgt cgaggggagc gaggccgact gggtcggcga
     1561 ccgctccggc ctcctcgtcg gccggcagct gatgaagcgg tacggctggc ggctcggtca
     1621 gaaggtctcc ttcaaggggg acatctaccc catcaccctc gagctgacgg tccgggccgt
     1681 cttcgcgggc cccgacgaga gcggcgtcta cttccaccac gccgcgatcg aggaggcgct
     1741 cccgcgggtg aaggggtacg tcggctggta ctggctgaag acggcctccg tcgccgacac
     1801 ggagcggatc ccgaggcaga tcgacgcgat gttcgagaac tcctcggccc cgacgcggac
     1861 cgagacggag aaggagttcc agagcatgtg ggtctcgatg ctcgggaacg tgaagctcct
     1921 cgtcggctcg atcacgacga tcatcggcgt cgtcatcctc ctcatcgcgg cgaacacgat
     1981 ggcgatggcg gcgcgcgagc gggtcaccga gatcgccgtc ctccgcgtcc tcgggttcac
     2041 gaaggagaag gtcctcggcc tcgtcctcgg cgagagcttt gccctctcga tgatcggggg
     2101 cggcctcggc ctcggcctct tcgtcctcct cctgcccggc ttccgcgagg ggctcgtcaa
     2161 ctccccgatg ggggccttcg ccgccgggat ccgcctcttc cccgaggtgc tcgccctcgg
     2221 cttcggcgtg accttcgccg tcggccttct cgcggggctc gtcccggcgg tccgctcggc
     2281 gcagcgcccg atcaccgaag ggctccggca ggtcgcatga agttcgtccc gtacgtcctg
     2341 aagagcctct tccgcaagaa gacgcgctcg gtcctgacga tcctctcgat cgtccttccc
     2401 ttcttcgtca tctgcgtcct cgggacgctc ctcgcgacgc tcgatgccga cccttcgggc
     2461 gggaagggga tgttccgcct cgtcgtgagg cacaaggtct ccctcgcgaa cttcatcccc
     2521 gaggcctacc gggagcggat cgcgcagctc cccggcgtcg aggaggtgac gatcctcaac
     2581 tggttcgggg ggacctacaa ggacaaccgc cccgagaaca tgttcgcccg cttcggctgc
     2641 gagccggaga agctcgtgag gatcttcgac gaggtgacga tcgtcgaggg gacggcggcc
     2701 gggtgggcct ccgaccggaa gggggccctc gtcggcgagc gcctcatgaa gcgctacggc
     2761 tggaagatcg gcgacacgtt cgtcctgaag ggcgacatct tcccgatgaa cctcgagctg
     2821 acggtccgcg ccgtcttccg cggctccgac gagacgggcg tctacttcga ccggaaggcc
     2881 gtcgaggagg ccctcccctt cgcgaagggg caggtcggga cgttctggat caaggccgcc
     2941 tccgccgagg cggtccagag cctcccccgg cagatcgacg ccctcttcga gaactcctcc
     3001 ttcccgacga agacggagac ggagaaggag ttccagaacg gcttcgtctc gatgctcggg
     3061 aacgtgaagg cggtcgtgac cggcatctcg accgcgatcg ccctcgtcat cctcctcatc
     3121 tcggcgaaca cgatggcgat ggccgcgcgc gaacgggtcg tcgagatcgc cgtcctccgc
     3181 accctcggct tcggaaagcc gctcatcctg acgctggtcc tcgccgagag cctccttctc
     3241 tcggccacgg gggccttcgt cggcctcggc ctcttccggc tctccttccc cgcgctgaag
     3301 gaagggctcc tcagctcgcc gatggccggc ttcgccgcgg ggatgcagct cttcccggag
     3361 gtcctcgcca cggggatcgc ggtaacgctc ctcgtcggcc tcgtcgccgg gatcgtcccg
     3421 gccatccgct ccgcccggcg ctccatcccc gacggcctcc ggcaggtcgg gtagagagga
     3481 atcgaacgtg ctgtcgatac cgctgaagta caacgtccgg aacctcttcg tccgcaaggt
     3541 gacgacgtcg ctgaccgtcc tcggcatcgc gctcgtcgtc gccgtcttcc tctgcgtcat
     3601 ggcgctcggc gagggcctcg cgaccgtatt caccgcctcg gggtcggaga agaacgtcct
     3661 cgtcctccgc cagaactcgc agtcggagct ccagtcgggg atctcgcgcg accaggcgcc
     3721 gctcgtgaag gcgctccccg gcatcgagaa ggacgccgac ggcaagccgc tcgtctcggc
     3781 cgagctcgtc gtcgtcctga acctcgacaa gcgggacggc ggctcctcga acgtcacgct
     3841 ccgcggcgtc gaggagaagg ggatggcgct ccgccccggg atgcggatca ccgaggggcg
     3901 ggcgttcgtg ccgggaacgt cggaggtcgt cgtcgccgag agcgtcgccc gcaggttcaa
     3961 gggggccgag gtgggccagg agatccggtt cggcgcctac cgctggaagg tcgtcgggca
     4021 cttcgacgcc ggcgggacgg cccccgacag cgagatctgg accgacaccg aggggatgct
     4081 gaacacgttc cagcgcggcg gctactcctc gatcctcgcg cgcacgacgg accgcgccgc
     4141 gcgcgaccag ctcgtcgcgg cgctcgccgg cgacgcgcgc ctcgccctcg acgggaaggt
     4201 cgagagggcg tactacgacg agcagacggc gaccgccgcg ccgatcaggt tcctcggcgt
     4261 cttcgtcggc gtcgtcatgg cgatcggggc ctgcttcggc gcgatgaaca cgatgtacgc
     4321 ggccgtctcg gcccggaccc gcgagatcgc gacgctgcgc gcgctcggct tctcgcgcct
     4381 ctccatcctc gtctccttcg tcctggagtc ggtcgcgctc gccctcctcg gaggcgtcgc
     4441 cggctgcgcg ctcgcgttcc tcctcgtgaa gctcgcgctc tcgggcgtga ccgggacgac
     4501 gaacttcgcc acgttcgccg aggtcgtctt cgccttccgg ctgacgccgg cgctcctcct
     4561 gacgggaatc gtcttctctc tcgtgatggg cttcttcggc gggctcttcc cggcgggccg
     4621 ggcggccttc acgaagatca ccgccgcgct ccggcaggtg ggctgagggc gccgcccggg
     4681 cgctcccgga gagtcacgcg atgggcgggc ggcgcttcgg cgccgcccgc ggcgtctcag
     4741 cccttccctt ccggacgctc cggggcgagg agccacgcgg cgcccgcgag gcccgcgacg
     4801 ccgagcaggt accagagcga gccgcttccg tcccgcgtct cggcgtagcc ggccgccgag
     4861 aggaggagcc cgaccgccga gccgacggcg agtaggagga gcgctccgcc cgcgaccttg
     4921 agcaggccgg ggagagcggc cgtccgcgtg ccgagcgccg ccaggatgag gcctccgaac
     4981 gcgcaggccg ggaagaggat ccaggcggag agtcccggcg acttcggcgt cagcccgaag
     5041 gagcccgcga gaaggaagac gccggcgccg aggccgacga gggcgaagac gactcccacg
     5101 attctcagca cgctccgatg cctcctgctc ccggaattat ccgccgcgtt ccgacgccgc
     5161 gggacccgct cccgcgcctc ggaccgtcag cgccccgcct gccaggcgta gacgagatcc
     5221 gaggcccggt cgaacgcgac gaaggtcccc agcgggaccg ccgcgccccg gccggagtgg
     5281 acgtccgcgg cgatcacggt cgtctccctg gccggaacca cctggacgag gccgcggaac
     5341 caccagtcgg cgcctttcgg cgcccggaag gtgagagtcg cgacctgggt gtcggggatg
     5401 cgcacgagct ccggctcgag cgccgtcctt ccccccttcg ggagacggaa acgcagccag
     5461 aaggctccgc tcccctcctc cgtcgccagc cggagctcgg tcgcgccggc cggaacccat
     5521 tctgcgacgg ccgcgccggc ctcggccctc gtggcgaaat tcctcgtcgc cgtcgcgccg
     5581 ccgcagcccg ccgccgcgag gaggacgagg acgagtggga ggcgaacgag gcggctcacg
     5641 tcgccccgtt cgctactggc aggcgccgat gttctcgcgg acgtactcga tcagcgtgcc
     5701 gtccttggcc cgggaccgga gcttgtccag gatcggccag cgccagcgcg gcttcgcctt
     5761 aaggatgctc gcgtagagac ggagcgtgct ctcgatcccc gcggcgtaca tcgcctcgcg
     5821 atccgaggcc ttgtcggggt tctggatcgc gaaggccgcg ttgccgaagt agagctgccc
     5881 gatgatctcc ggggcgccgt tcttgccctg gccgttcagc gcgccgagga gctcggggca
     5941 ggtggaaacg aagaggtccg gcgcgttgac gatgaagttc gtcaaccact gacggctgtt
     6001 gtccgcgccc gaggcgagcg ggtccgcttc gagctgctgg acgaaggcga gagcctgcgc
     6061 cttgtcttcc gcggaggaga cggttgcttc ctgcgcgtac gacggcgtgg cgacgacgaa
     6121 tgcggtcagg agggccggca gcgcgagcgg cgaaaggaga gacctcttca tggggtcgtt
     6181 actatgccct aaaaatgtct tcagatcatc cccggtccgg aatctgcctt tcgccagccg
     6241 cctcgtcagc ctccgacgcg ggcccggagg gcgaggagga gcccggcgag cagcgcggag
     6301 ccggccaggc acgtcccgaa gagccagagg tcgatccttc cgtagcccgc gagagcaggc
     6361 gctccgcgcg cgatccacag gccgcgggcc gacaggccga tgcagagggc cgcggggagg
     6421 gcgacggcgc cccacggcag gcccccggac gaggcgacga tcaccaggaa gccggcgacg
     6481 agccccgagg ccacgacgcc ggcggccggc aggaacaggc ggctcgactc gaagcgcccc
     6541 tggacgaaca cggcggcgag ggccaccgaa gccaccccga acgcgacgac gccgcacgag
     6601 aacgcgagga cgaacgaggg gaaggacggg tccacggccc ttcctcagga cgcccggatt
     6661 cccggccggt acgtccgcgt cacgctcgag ccgccccggc cggaagcttc cccaggaggc
     6721 gccagaggag acggtggaac ccgtggatgc cgccggcctg cggcaggaac atcaggaggc
     6781 agtggccccg caccagcgca acgccccgtt cggccgcgat tcggacggcc tcgtccgatt
     6841 cggccccctg ctggagccag acgcgaccga tcccggcgtc ggcggcctcc cggacgagct
     6901 tcacggcctc ggaagggggc gtgacgacga cgagcccgtc gacccggccc ttcagctccg
     6961 cgaggccctt tgcgcacgga agtccctgga ggtccggggc ctcgggatgg accggaacga
     7021 cctcgtagcc gcgggccttc agctcccgga ggagcaggtt cccgaacttc cggcccgacc
     7081 gcgaaactcc cgcgatcgcg agccgccgcc gcgaggcgaa gtcctggacc agctcggagc
     7141 tcacgaccgg ttcacccatc atggaaacct ccgcgtccgc cctcggggcg gacgacgctc
     7201 gtcgcgcgct actccacgag gaggagctgc gccgccacgg cgagggcggc gaggcgccct
     7261 cctccaggcc ctccctccga cttccgctcc acgtagagga ggactccgaa gtcggggaag
     7321 tcgggctccg cgaaggcgtc gtccgggtct ccggaatcct cgtcgaagtc cgggtcgagg
     7381 ttcgggcgga gcgtcagctc cgactccggc tcgtcgagct cggcgaagta gtacggcccg
     7441 atcgactcgc tctcgtgcag acggaacgac acgccgagga gccgctcggc ctgctcgcgg
     7501 gccgcctcga cgtcgtcgga gacgaacccc cagagctcga cggtctcgct catggccgat
     7561 ccgcccgccc gcccctcagc cgcgccccgc cacggcgtgg cgctcccaga aggcgatgtc
     7621 gggcgcctcc gcgaacgagg ccggggccgc ctcgatgaag gccttcaggt gcggtgtctg
     7681 gaaatgcggt ccgagatacg cctcccgcga ggtccaggtc tcgtacagga ggatccgccc
     7741 ccggtcggat tcgtacgtga ggagctcgat gctgaggcag tccggctcct ccctcacgac
     7801 ggtcgcgatc agctccgaga ggagcgcgag ggcttcgtcc tctttcccgg ggagggcctg
     7861 gtagcggatc aggacggtga tcgggtctgc ggccatcggc tctcctccta gcccttccgc
     7921 cccgcgacga ggaggagggc gcccgcgacg accgcacccg cgcccgccca gaccgggacc
     7981 tcgaacgtcc ccttctcctt cacggcgagc tcgaggccga ggaccttcgc ctcgtgcgtc
     8041 ttcttcgtgt aggtgaacga ccggtacgcg agggccacga cgccgagcgc gacgaggaga
     8101 agccccacga tcactttcgc cttcatcccg atctccttcg gcgccgtcga gccgtccgtc
     8161 cccgcgaagt cggatccgtc tctcgtccgg cggccctcgc ttcgcgcgga ggatagaccg
     8221 ggacgcgagg atgcggcacc ccgggccgga cgtcgccggt cagaaggcgc cctcggcctt
     8281 catccgcgcc agccccgatt ccgtgagcgc ggcgagctcg gcgaagagcg ccgcgtcggg
     8341 cgcgcggaag ttgaagcacg acttcccctg catccgcgcc ttcagctccg gagtcgcccc
     8401 ttcgaggagg tccgggtaca tgtagaccgg gaagaggtgg aacgagacgt agttcttcgt
     8461 cacctggacc gcgccgaaga aaagctcctt cttccaccga gcggagtagg gggtgttcag
     8521 cgagtagcct ttgggagagt ccgcggtgac cttcagctcc tccgcgtgga ccgagaggat
     8581 cgcccggagc gccgcgaaga cctcggcgga ctgctcggcg gtggcgcgga cggggggcgt
     8641 gccgtggact ctcgcggaca tgacgacctc ctgcggctct cggcgcacgg agaggctaac
     8701 agcgtccggc gggcttcggt cgccggaagg cgcggatcgg tccggaagga cgggacggcg
     8761 ccccggcgca gagcgtcgcg gagcgcgcga gggcggggct caggcctgct tcgacctgcc
     8821 ccggacgagg gtgtagacga ccaggaggac gatcgcgccg acgacggcca tcaggaatcc
     8881 cgccgcctcg ccctccttgt agaagccgac gagccgtccg aggaacgtgg cgacgaggga
     8941 gcccgcgatg ccgagggccg tcgtcaggat gaggcccagg ttctccttgc cggggtggag
     9001 gaacttcgcg atgacgccga tgacgaagcc gatgaggatg gtccacagga tgctcagcat
     9061 gaggggattg tccgctcccg gccggcgcgc cgcaattcgg cgcggtcagg cgcggcccga
     9121 ccagccccag atcgcgacga agtgacaggc gctccccgcg aggacgaaga ggtgccagat
     9181 cgtgtgcgcc cagcggaccc tcttcgcggc gtagaacgcg atgcctcccg tgtagaggag
     9241 cccgcccgcc gcgagccagg ccaggcccgg ccacgggacg gaggcgagga gcggccgggc
     9301 cgcgacgacc acgagccacc ccatgacgac gtagagcgcg acggcgacct tgtggacctt
     9361 cttgccgaga gcgaactcga gcgcgatccc ggtcccggcg atcgcccaga cgagggagaa
     9421 gagggtccac ccccacggcc cccgcaggac cccgagggtg aacggcgtgt acgagccggc
     9481 gatcaggagg tagatcgccg tgtggtcgag cttctggagg acctgcttgg cacgggaggg
     9541 ccgtagggcg tggtacaggg tcgagaagag gtagagggcg acgagggtcg ccgcgaagac
     9601 ggaggcgccg acgacctcgg gcgctcccca tcggcgggcc gccgccacga cgaggaacgg
     9661 gaacgcgacg acgctcgcca ggagaccgat gccgtgtgtc acgctgttgg cgatctcctc
     9721 gcccctggtc aggggacggg gaggggcggc gaggacgttc atcgggacgg gaggataccc
     9781 cgtgccgccc ggcgggaccg catcggctca gcgggcgacg cgtcgcaggt cgccgccgag
     9841 gtaccggcga aaggcgccgc ccgggggcgc gacctcgaac gtcaccgtca tctcgtccgg
     9901 cgccgggcat gcgtaggtca ggcggaaccg gggcccgggg gccgccgcgt cgctcacgag
     9961 ggtgaggacg gctccgtcgg ccgaccagga cgcgtcgtac cggatgacgt ggccctcgtt
    10021 gtcgaagtag acggccgacc cggtcgaagg agtcgcaccc ggggagacga cgaggaggtc
    10081 ctcgtggtgc gcggccggcc ggccttcggc cgccgggtag tccgccacgt tgcgccggac
    10141 gaggacgtgc ccgtcgagct cgagccggaa ggaggacccg ccgccgccgg ccccgggggc
    10201 gccgctcccg ccgcctttcc attcgcccag gagcttcctc cactcggggc cgaacgccgg
    10261 ggcccctgtc tctcggggag gcgcggacga aggggtcgcg gaggccgacg gcggcccggc
    10321 gagggcgagg aggacgagcg cgaggatcat ggccgcgagg atctcacggc ggggcgggcg
    10381 cgggccggcg gcggaggcct cgaagagacc gggggcggcc ctgtcgggcc gcccccggtc
    10441 gggacgacgg aatcggggag ggactacttc tcgaggccct ccgtcccctc gtcctcgcgc
    10501 ttgctctgcc agaccgccca gccgatggcg gcgagggcga cgaggacgac gacgacgccc
    10561 gtgacgcggg agccgccggc cggagagagg aggttgtgct gcaggacgag cccgagcgtc
    10621 aggaggctca ccatgttcat gaccttgatg agcgggttga tcgcgggtcc ggccgtgtcc
    10681 ttcagcgggt cgccgacggt gtcgcccgtg acggccgcct tgtgggcctc cgagcccttg
    10741 ccgccgtaga tgccgtcctc gatgagcttc ttggcgttgt cccacgcgcc gcccgcgttc
    10801 gccatgaaga cggcgaggag ctggccgacg aggatcatgc cggcgaggaa gccgccgagg
    10861 gcgtacggcc cgaggaggaa gccgacgagg atcggggtca tgatggcgag gaagccgggc
    10921 ccgatcagct ccttctgcgc ggtcgtcgtg cagatcgcca cgacgcggcc gtagtcgggc
    10981 ttcttcgtcc cggcccagat cgccgcgtcg cggaactgca cgcggcactc gttgacgatg
    11041 tagaaggccg cgcggccgac ggcgcggatg agcatcgagg agaagaggaa cggcacggcg
    11101 ccgccgagga ggaacccgat gaagaccttc gggtcggcga cggtcaggcg ccccgcctcg
    11161 gcgaggtact gcgccgtcgt catctcgttg atcttctcct cgctcccgac cgcgatgacg
    11221 gcgatgaagc tcgagaagag cgagacggcc gcgatgacgg ccgagccgat cgcgattccc
    11281 ttcgtctcgg ccttcgtggt gttgccgacc gcgtcgaggt ccgcgaggat ctggcgggcg
    11341 cgcttggccg agccgggggc cttcgcgtcc atctccttga cgtcgtaggc catctcgccg
    11401 atgccgttcg cgttgtcggc gaccggcccg aagacgtcca tcgagatcgt gttccccgtc
    11461 agcgtcagca tgccgatgcc ggccatcgcg acgccgtagg cgatgaagag cgggggcgtc
    11521 ccggcgtagc agaggaccga gaggccgatc gcgcccgcga tgacgatgat cgccatgacg
    11581 gtcgactcgt agccgacggc gaagccctgg atgatgttcg tcgcgtgccc ggtctggcag
    11641 gaacgggcga gccccttcac gggggcgtgc cccgtgtggg tgaagtacga ggtgaccttg
    11701 ttcagcgcga tcgcgaggaa gacgccgatg aggcaggtga gcgcggggcg gaggtcgagg
    11761 ccggggatgc cccagttcag cagcccgccg agcgccgtcg ggtcgccgtt cgggaagccc
    11821 gccttcgcca tcgggttcgc ctcgaggtac gccgcgtcga agcggaggta cgagaagccg
    11881 agcgcgaaga agccgccgat gctgatgagc gagccgatcc agaagccgcg gtggacgctc
    11941 ttgagcgcgg tgtcggaggt gtcgttcggc ccggccttga ccgtgtaggt cgagatgatc
    12001 gagccgagga cgccgatgcc gcggacgagg agcgggaaga tgaccccctt gtggccgaac
    12061 gtggccatgc cgaggatcat cgcggcgacg atcgtcacct cgtagctctc gaagatgtcg
    12121 gccgccatgc cggcgcagtc gccgacgttg tcgccgacgt tgtcggcgat cgtcgcggcg
    12181 ttgcgcgggt cgtcctcggg gatgtccttc tcgatcttgc cgacgaggtc ggcgccgacg
    12241 tcggccgcct tcgtgtagat gccgccgccg acgcgcatga agagcgcgag gagcgtgccg
    12301 ccgaagccga agccgaggag cgcctcgtag gccttctcgc tgaagatcag gaagatgatc
    12361 gtgccgccga ggaggccgag gccgtccgtc agcatgccgg tgaccgtgcc ggtccggtag
    12421 ccgagctgca tcgcctcgcc gtagctcgag cgcgcggccg cggcgacgcg gaggttgccc
    12481 gccgtcgcga ggcgcatccc gacgaagccg accgcccagg agaagagcgc gccgacgagg
    12541 aacgcgcccg cgcggcccca gcggaaggcg ttctcgctcc ccgtgtacgt cgcgaagagg
    12601 aggatcgtca ggacgatgat cagcggtccg atccgcttga actgcgccgc gaggtaggcg
    12661 ttcgagccct cccggacggc ggcggcgacc tcctgcatcc tcggcgtgcc ggtgtcggcc
    12721 gcgaggacct gccgggcgag gaagagcgcg tagaggaggc cggcgaccgc gacgccgagg
    12781 accgccatga gcgccccgat ctcgaggggc gtgtagcgcg ggtcggagaa gagcgcgaac
    12841 ggcttgaagg gggccgcctc gccaccgccc gatccgaaga gcacgggcga cagcaggaac
    12901 gcgccgcctc cgaggaccgc gaaagccgtg aggcgtccgg tgctcatcgg ggacccttgc
    12961 cgcctgaaaa aggacatcag gaaagctcgc aacgtcgtac ctcctccacg ggacagcgga
    13021 cgcccttgca gcacactccg gagaagccgc ccgctcttca gggcgcgaac ggtacgcttt
    13081 cctctatcgg gcggcaagcg aggaagcggc ctgaattggg gtcgtcccgg tcccgtttcc
    13141 ggtgcgccgg gcccggccgt tacttgtagg agaagccgct cccgccgatg aactcccgga
    13201 ggatgctcgt cgccgggacg tcgtcgggcc agaccgggac gaacagctcg aggaagcgcc
    13261 ggagctggta ccccttcgcc tgagccggcg agtcgggcgg gacctcgagg aggtagcgat
    13321 aggcgaagtt cctgaggacg gccgtctcgt acgcgagcgc cgagttgaac atcgcgtcgc
    13381 agcgttcctg gaacgggaag atgtgcttct cctcgccggc gcggaccttc ggccagcgca
    13441 ggatcgacgt ctcggccgag tagttccggt acc
//
LOCUS       DAOTEZ010000064        13449 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3186_length_13449_cov_6.141332, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000064 DAOTEZ010000000
VERSION     DAOTEZ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13449
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3186_length_13449_cov_6.141332"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            314..3016
                     /locus_tag="P5164_05150"
     CDS             314..3016
                     /locus_tag="P5164_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRY43316.1"
                     /translation="MTVASGTRLGPYEVGTPLGAGGMGEVYRARDTKLGREAAIKVLP
                     PAFAEDAERLGRFRREAQILASLSHPNVAAIYGLEESEGLVALAMELVAGEDLSERLA
                     RGPFPVDEALAVARQVAEALEEAHGKGVVHRDLKPANVKLAPDGKVKVLDFGLAKALS
                     GEAPAALDSSDSSRSPTMTRQGTQAGMILGTAAYMSPEQARGKPVDRRADVWSFGVVL
                     FEMLTGRRLFAGETVSDTLAAVLRADPDWALLPPGLPAPVGDLLRRCLERDPRRRLHD
                     VADARIVIEDVLEGRWRPTAATAPLPPVATARPPTARRAAVPLAAFLLGILVAVPVLS
                     SIRRGPGTPPPSKQPAGFRQLTFLAGGEMAPALSPDGESFAYVKEVGGQRDVFLQRVG
                     GTNPVNLTAGCEADDDEPAFSPDGRRIAYRSECAGGGIFVMGATGESARKVTDAGCDP
                     AWSPDGTELAVAGEKLRSPISRTTTSPLFAVKVGTGERRLLSSHDAVEPSWSPDGRRV
                     AFWGLVDDTPVRDLFTVAAGGSQSEPSAAVRLLSDRHVDWSPNWTENGRALFFASSRG
                     GTMNLWRIGVDPGTGRPLGKPEPVTAPSSWVGYLSASANGRRVAFVDRNVRTKVRIAP
                     FDPAAGRIAGPPREAPLGTVEANGPVSLSADGATLLFDDSGFPQRLVLVEPGGTMRQL
                     TEGPHRDRQGAFSPDGAWIAFQTDRWKGSLALIRPDGSGLSEVLPGRSASVFQPSWSP
                     DSRWLAAGNTDADGPFLLEVADGKAAGPPQFLAPLEEDGLDFWPSSFSPDGRRLAGAL
                     YSAAAHPVGGAVYSLDGRTYRRLPTGDAGALLFLPDGRRLLLVSRREIRLFDLEKGGL
                     RTLVTAPEGGEIGSAALSPDGRVLAWHESTDESDIWLAEFEEKE"
     gene            3087..3809
                     /locus_tag="P5164_05155"
     CDS             3087..3809
                     /locus_tag="P5164_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43317.1"
                     /translation="MRAVLGALSGLAGAVVGGLVAALLATVFAKAANVTTREGAAGYL
                     VVAVGVLGALAGLVVGVVLYARAAPAGEGMRAAGSALLGLLGLGVLAAALLWAFTLSH
                     EGPAKYGDTLASLELELRVRKADAPAGSPSSWLDVEVQTAKTRPVAVVLSDRVREEGD
                     AIVVPLVQNPLHRASGRLVVVRVADRHVEVFSPRWRAKPDPKADWSPWIAPRLAERTG
                     GEAREAVRPILQLRYRVRLYGD"
     gene            3863..5212
                     /locus_tag="P5164_05160"
     CDS             3863..5212
                     /locus_tag="P5164_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HRY43318.1"
                     /translation="MDSNASARRRAHLDRIRDAAVAAVEPARAVARFLSTEGGDLRAG
                     DARVPLSADSSVRIVAAGKAAAAMTAAAASILGHRIERGVLVTKHGHLVGRTVPPAID
                     AFEAGHPVPDETGREAVSAVEALLSGGREGDVVLALLSGGASALLADPAEPITLADLQ
                     EATGLLLRSGASIVELNAVRKHLSRLKGGQLARLAAPATVVSLILSDVVGDPLDVIAS
                     GPTAPDPTTFADAWAVVERRDLAGVLPASVRARLCEGAEGRLPDTPKPGDALFGRVHN
                     VLVGSNRLAALAAVDEARALGYTTLLLSTFVEGEAREVARVVAALGRGVLAHGDPVPP
                     PACLVFGGETTVTVRGEGRGGRNQELALAAALALEGIDGVSVMALATDGSDGPTDSSG
                     AIVDGATAGAIRLAGLDPAAALAANDAYPALAAAGSQLKTGPTGTNVNDLTVVVVGG"
     gene            complement(5241..6188)
                     /locus_tag="P5164_05165"
     CDS             complement(5241..6188)
                     /locus_tag="P5164_05165"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRY43319.1"
                     /translation="MSDYLWNPAHEPDPDVRAIEERLAPLRREPPSLRELPDRSPVLP
                     PSWRRLFVAAPALLAACLLVAASLALLARGKGWEVRWEGERTTRLARGEWLETGGATA
                     RLSVGRIGTLTLEPGTRLRLVSDSPRDHRVALARGTVRAEIQAPPRRFFVETPAATAI
                     DLGCAYTLSTDEAGNGLLRVRAGWVSFERRTAGALDEVVVPAGASCRVSARRGPGVPA
                     WDDAPEPFLSALARWEESAESAGDAPPLDDLLRTARPRDGLTLLGLAPRLDAAGRAAV
                     FDRLAALSPTAALPSRDRFVAGDAGAREALRGVLGLPTP"
     gene            complement(6185..6718)
                     /locus_tag="P5164_05170"
     CDS             complement(6185..6718)
                     /locus_tag="P5164_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRY43320.1"
                     /translation="MGLRLAAPGPAASGAELVAAARDGDRDAFDELYRRYWRLVRAVL
                     LARLPRPDAEDLAQEVFLAAWEKLGDLRDPEGFGGWVAAIARNRAASHVRSAPRHAPL
                     PDDLPGGARADEGPGAVLAAIRLLPEAYREPLVLRLLEGMSGPEIARETGLTEGSVRV
                     NLHRGFAKLRGLLGGRR"
     gene            6883..7524
                     /locus_tag="P5164_05175"
     CDS             6883..7524
                     /locus_tag="P5164_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43321.1"
                     /translation="MNDRPVPTRRRFLALGLGAAAAAWGGALAASRDDDPCLVARPEA
                     RLAPPGEPGSPLRVEGLVLAPDGTTPAAGVVVYAYQTDATGVYAPPGRPPRLKGWMRT
                     DSDGRFAYDTIRPAPYPGGRNPAHVHHQLWGAGWPAQWGNDLLFDDDPLVPESERRRS
                     ASLGPFAFVRAVEEHPLHGALVKLRLRLRTAGDRFEDGTRHGVDACRHGRGGR"
     gene            7665..8816
                     /locus_tag="P5164_05180"
     CDS             7665..8816
                     /locus_tag="P5164_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43322.1"
                     /translation="MPETITALELSALVSRVFQPTAEDTRLGIMVDLPDEKVLDDAPW
                     AARRAIAAEWAELLGMQVREAGYETRLLLYPNVHTNNGDLPGRCWIHAGGPVPASAAE
                     LDPAASVAMTEIFDTFPMLLALTKFSSTAPLKMAAKKHPMRAATMPGFSAAMIPALRL
                     DYGEVNRRVDILKAHLDRAIGADFVFRRPAGEDRLHLDLRHRTGHASGGLLPKRGVAG
                     NLPSGESYIVPYEGEVEGDPSRSEGVLPVQFGKEVVRYRVERNRAVEVLTRGPESERE
                     AALLAKEPAYANLAELGLGVLAAFGVKPVGSVLLDEKLGLHIAFGRSEHFGGQVGPDA
                     FSSPEAVVHIDRVYVPETQPDVLPAIVDLVFEGGERLPLMRDGEFGPVF"
     gene            8908..9456
                     /locus_tag="P5164_05185"
     CDS             8908..9456
                     /locus_tag="P5164_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43323.1"
                     /translation="MSSRAGTALAACALVLSLVAPAAPLSSVTCNGVEMPVKAGVAVW
                     DAKGTTLQLFLLPFEPTAAETERLGRGDTTWLDKRAPVDPKKWPRWNPRADLLLSWSW
                     NPKAVGDASKLNMCTLFQYDIAKNAVNSFVDCPDQKLNGRLEGRLAAGESITVASRGE
                     GKNGDYALSWDFHVTTKVLSKP"
     gene            complement(9513..10031)
                     /locus_tag="P5164_05190"
     CDS             complement(9513..10031)
                     /locus_tag="P5164_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HRY43324.1"
                     /translation="MPAGSDPSDRPGGLAEVDWSRWEPTEHAVLCFVRDGDLLLLIHK
                     KTGLGAGKVNAPGGRIDPGETAEEAAIREVREEVGVEASRLTKAGELSFQFVDGYALH
                     GTVFFAASHSGTPRETREADPFWCPVGEIPYERMWEDDRYWLPLALEGKPFRAFFVFD
                     GDRMLSRRIDLA"
     gene            complement(10015..12054)
                     /locus_tag="P5164_05195"
     CDS             complement(10015..12054)
                     /locus_tag="P5164_05195"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HRY43325.1"
                     /translation="MSRPAPYPHLLAPLDLGFTTLRNRVLMGSMHTGLEDRTSRFPRL
                     AAYFAARARGGAGLIVTGGFAPNVEGWLSPFGSRLASARAARAHRPITDAVHAEGGKI
                     ALQILHSGRYGYTPLAVAPSRVKSPISPFTPRALSARGVERQIRAFVRCGVLAREAGY
                     DGVEVMGSEGYFLNEFLVTRTNKRTDRWGGPYENRMRLPVEVVSRVREAVGKDFILVF
                     RISMLDLVPDGSSWEEVVALAKAVERAGATILNTGIGWHEARVPTIATSVPRAAFAWI
                     TRKLKGEVGIPLCTTNRINAPEVAEEILASGGADMVSMARPLLADPDFVRKAAEGRAD
                     EINTCIACNQACLDHVFDGREVSCLVNPRACRETVVVFPVAAARKRIAVVGAGPAGLA
                     CATELAARGHAVDLFEAVGEIGGQFNLAKRVPGKEEFAETLRYFARRIETTGVSLKRG
                     TRATAEALVAGKYDDVVLATGVVPRSPGIPGQEHPKVVSYVDVLLGRKSVGERVAIVG
                     AGGIGFDVATFLVEGAEGHGRDLERWKAEWGVADPETARGGLVKPRPAKAARRVTLLQ
                     RKATKPGAGLAKTTGWIHRAALKAKGVEMLAGVRYERIDDAGLTITRGTKSPETRLLE
                     VDTVVLCAGQEPLRELEAPLREAGVSVHLIGGADVAAELDAKRAIEQGTLLAGRL"
     gene            complement(12119..12913)
                     /locus_tag="P5164_05200"
     CDS             complement(12119..12913)
                     /locus_tag="P5164_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43326.1"
                     /translation="MRLRQLRAVFVLELKRLFRGREGRATLFFALAPVFLAIVGLTAR
                     NTPLTAGRAENAFAFIFQNLVLQGTLYFGCLFTFGGLVRGEQIAKTLHHLLLAPVRRE
                     VVLLGKYLAACAVTATLFGLTSAASYLLLLSDSGRPAMLAHLGAGGAGRTAAYVFVAV
                     LACAAYGAVFLAFGQWVKNPIFPALAFWGWEHLNFLLPEGLKRLGLIHYILSLTPVRV
                     PESFLAILGEPLPVWVSIPVPLLFTAALLAFAAWKVRRTEVDYGIE"
     gene            complement(12922..>13449)
                     /locus_tag="P5164_05205"
     CDS             complement(12922..>13449)
                     /locus_tag="P5164_05205"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY43327.1"
                     /translation="MKQRLRLAAAIAPDPRVLLLDEPLNGLDPMARAEVLDLFRTLAA
                     EGKHVVVSSHILHEVDLLADRVVLVKSGYVVAEGESHGLREEVVERPMQVLVRCDRPG
                     ALAARLFTEGLVTEVELHEDGGGLLARTRTPDRFFLALNDAVLTDGLEVETVTPADDD
                     VKAVYRYLIEGGEGE"
BASE COUNT         1743 a         5138 c         4850 g         1718 t
ORIGIN      
        1 cgaggagctt ctctcggcgg tcgaggagat cgggccggaa atcgatctgc gggatggaca
       61 gagcctgctc catcgtctcc tcctggaacg gttcccgttc tacgcagacg ggggtcaccg
      121 ggttctggcg gagggcgctc cccgggaccc gggccccggg gccgaagccg tgtccggggc
      181 ttcgacccgg gaccgcgatg gatgtgaagg tcggcccgat cgggacgagc cggagcgcgc
      241 aagcccggtt ccggcgcgtt tgcacgggaa ttgcgttttc gcgagccacc ctccgtagaa
      301 tccgccgcgt tcgatgacag tcgcatccgg cacccggctc ggtccgtacg aggtcggaac
      361 gcccctcggc gcggggggaa tgggcgaggt ctaccgcgcg cgggacacga agctggggcg
      421 cgaggcggcg atcaaggtcc tgccgcccgc cttcgccgag gacgccgagc gcctcggccg
      481 gttccggcgc gaggcccaga tcctcgcctc gctcagccac ccgaacgtcg ccgccatcta
      541 cggcctcgag gagagcgagg gcctcgtggc gctggcgatg gagctcgtgg cgggcgaaga
      601 cctgtcggag cggctcgcgc gcggcccctt tcccgtcgac gaggcgctcg ccgtcgcgcg
      661 gcaggtcgcc gaggcgctcg aggaggccca cgggaaggga gtcgtccacc gcgacctcaa
      721 gcccgccaac gtcaagctcg cgcccgacgg caaggtcaag gtcctcgact tcggcctggc
      781 gaaggccctc tcgggggagg cccccgccgc tctcgactcg agcgactcct cccggtcccc
      841 gacgatgacg cgccagggga cgcaagccgg gatgatcctc gggaccgcgg cctacatgag
      901 ccccgagcag gcgcgcggca agccggtgga ccggcgggcc gacgtctggt ccttcggcgt
      961 cgtcctcttc gagatgctca cgggcaggag gctcttcgcc ggggagacgg tgagcgacac
     1021 gctcgccgcg gtccttcgcg ccgaccccga ctgggccctg ctcccgcccg gcctgccggc
     1081 gcccgtcggc gacctcctcc gccgctgcct cgagcgcgac ccgcgacgga ggctccacga
     1141 cgtggccgac gcccgcatcg tgatcgagga cgtcctcgag gggcgctggc ggccgacggc
     1201 ggcaacggcg cccctcccgc cggtcgcgac cgcgcgcccg cccacggcga ggcgcgcggc
     1261 cgtgccactc gccgccttcc tcctcggcat cctcgtggcg gtgcccgtcc tctcgtcgat
     1321 tcgacgcggc cccgggaccc ctcccccgtc gaagcagccc gccggctttc gtcagctgac
     1381 gttcctcgcc gggggcgaga tggcccccgc gctttcgccc gacggggaga gcttcgccta
     1441 cgtcaaggag gtcggcggac agcgggatgt cttcctgcag cgggtcgggg ggacgaaccc
     1501 cgtgaacctg accgcaggct gcgaggccga cgacgacgag ccggccttct cgcccgacgg
     1561 ccggcggatc gcctaccggt cggaatgcgc cggcggcggg atcttcgtca tgggcgcgac
     1621 gggcgagtcg gcgcggaagg tcaccgacgc gggctgcgac ccggcctggt cccccgacgg
     1681 cacggagctc gcggtcgccg gcgagaagct ccggagcccg atctcccgca ccacgacgag
     1741 cccgctcttc gcggtgaagg tcggtacggg cgagcgacgg cttctctcgt ctcacgacgc
     1801 cgtcgagccg agctggtcgc cggacgggcg gcgggtggcg ttctggggac tcgtcgacga
     1861 cacgcctgtc cgggacctct tcaccgtcgc ggccggcggc tcgcagtccg agccctccgc
     1921 cgccgtgcgg ctcctctcgg accggcacgt cgactggagc ccgaactgga cggagaacgg
     1981 ccgggccctc ttcttcgcga gctcccgggg cgggacgatg aacctctggc ggatcggcgt
     2041 cgacccgggg accgggcggc cgctcggaaa gccggagccg gtcacggcgc cctcgagctg
     2101 ggtgggctac ctctcggcgt cggcgaacgg ccggcgggtc gctttcgtcg accgcaacgt
     2161 ccggacgaag gtccgcatcg caccgttcga tccggccgcc ggcaggatcg cgggacctcc
     2221 ccgcgaggcc cctctcggga cggtcgaggc caacgggccc gtctcgctct ccgcggacgg
     2281 cgccacgctt ctcttcgacg actccggctt cccccagcgc ctcgttctcg tcgagcccgg
     2341 cgggacgatg cgccagctca ccgagggccc gcaccgcgat cgccaggggg cgttctctcc
     2401 cgacggcgcc tggatcgcgt tccagacgga ccgctggaag ggaagcctgg ccctcatccg
     2461 cccggacggc agcggtctct ccgaggtcct gccgggacgg tccgcgagcg tcttccagcc
     2521 gtcctggtcc cccgacagcc gctggctcgc cgcgggaaac acggatgcgg acggaccgtt
     2581 cctgctggag gtggcggacg gcaaggccgc gggccccccg cagttcctgg cgcctctcga
     2641 ggaggacggc ctcgacttct ggccctcgtc gttctccccc gacggccgcc ggctcgccgg
     2701 ggcgctctac tcggcggcgg cccaccctgt agggggcgcc gtctacagcc tcgacggccg
     2761 cacgtaccga cggctcccca cgggcgacgc cggcgcgctc ctcttcctcc cggacggccg
     2821 caggctcctc ctcgtgagcc ggcgcgagat ccggctcttc gacctcgaga agggcggctt
     2881 gcggacgctc gtcacggccc ccgaaggagg agagatcggc tcggccgccc tctcgcccga
     2941 cggacgcgtc ctcgcctggc acgagagcac cgacgaatcg gacatctggc tggccgagtt
     3001 cgaggagaag gaatgacgct cgcccccggc gtccgcatcg ggagctccga cgtcaccggc
     3061 aggcccggcg atccgaggct gggggcgtga gggccgtcct cggcgccctc tccggcctcg
     3121 cgggtgccgt cgtcggcggg ctcgtcgcgg cgctcctcgc aacggtcttc gcgaaggcgg
     3181 cgaacgtcac gacccgcgag ggggccgccg ggtatctcgt cgtcgccgtc ggggtcctcg
     3241 gggcgctcgc gggcctcgtc gtcggtgtcg tcctctacgc gagggccgct ccagcgggcg
     3301 aggggatgag ggccgccggc tccgccctgc tgggcctcct cggcctcggc gtcctcgccg
     3361 cggctctact gtgggccttc acgctctcgc acgaggggcc cgcgaagtac ggcgacactc
     3421 tcgcgtccct cgagctcgag cttcgcgtca ggaaggcgga tgcgccggcc ggatccccgt
     3481 cgagctggct cgacgtcgag gtccagacgg cgaagacccg gccggtcgcc gtcgtcctct
     3541 cggaccgggt gcgcgaggag ggcgacgcga tcgtcgttcc gctcgtccag aacccccttc
     3601 atcgcgcctc cggccgcctc gtcgtcgtcc gcgtcgcgga ccggcacgtc gaggtcttct
     3661 cgccgcggtg gagggcgaag ccggacccga aggccgactg gagcccctgg atcgcgccgc
     3721 gcctcgcgga gagaaccggc ggcgaggcgc gcgaggccgt gcggccgatc cttcagctcc
     3781 gctaccgcgt tcgcctctac ggcgactgac cgtcgtgccg gggaacggaa gggagggccc
     3841 gccacggcgc acaatgccgc gcatggactc gaacgcgagc gcccgccgcc gggctcacct
     3901 cgaccggatc cgcgacgccg ccgtcgccgc tgtcgagccg gcgagagcgg tcgcgcgctt
     3961 cctctcgacc gagggcggcg acctccgggc tggcgacgcc cgcgtgccgc tctccgccga
     4021 ctcatcggtc cggatcgtcg ccgccggcaa ggcggccgcc gcgatgaccg ccgccgccgc
     4081 gtcgatcctc ggacaccgga tcgagcgggg cgttctcgtg acgaagcacg ggcacctcgt
     4141 gggccggacg gtccctcccg cgatcgatgc cttcgaggcg gggcatccgg tccccgacga
     4201 gacgggtcgc gaggccgtct ccgccgtcga ggcgctcctc tcgggaggcc gcgagggcga
     4261 cgtcgtcctg gcgctcctct ccggcggagc ctccgccctc ctcgccgacc cggccgagcc
     4321 gatcacgctc gccgacctgc aggaggcgac cggactcctc ctccgctccg gagcgtcgat
     4381 cgtcgagctg aacgccgtga ggaagcacct ctcgcggctg aaaggcgggc agctcgcgcg
     4441 gctcgccgcg cccgccacgg tggtctcgct gatcctctcc gacgtcgtcg gcgacccgct
     4501 cgacgtcatc gcctccggac cgacggcgcc cgacccgacg acgttcgcgg acgcgtgggc
     4561 ggtcgtcgag cgccgcgacc tcgccggcgt gctccccgca tccgtccgcg cccgcctgtg
     4621 cgagggcgcg gagggacgcc tgcccgacac gccgaaaccg ggtgacgcgc tcttcggccg
     4681 cgtccacaac gtcctcgtcg gctcgaatcg cctcgccgcg ctcgcggctg tcgacgaggc
     4741 ccgcgcgctc ggctacacga cgctcctcct ctcgaccttc gtcgaggggg aggcgcgcga
     4801 ggtcgcccgc gtcgtcgccg ctctcggccg cggcgtcctc gcgcacggcg acccggtgcc
     4861 gccgcccgcc tgtctcgtct tcggcggcga gacgacggtc acggtgcgcg gggagggccg
     4921 cggcggccgg aaccaggagc tcgcgctcgc ggcggcgctc gcgctcgaag ggatcgacgg
     4981 ggtctcggtg atggcgctcg ccaccgacgg ctccgacggc ccgaccgact cctccggcgc
     5041 gatcgtcgac ggcgcgacgg cgggggcgat ccgcttggcg ggcctcgacc cggccgcggc
     5101 cctcgcggcg aacgacgcct acccggcgct cgcggcggcc ggcagccagt tgaagaccgg
     5161 gccgaccggc acgaacgtga acgacctcac cgtcgtcgtc gtcggaggct gagcgcgggc
     5221 gcgggggctc ccgcgccctt tcacggcgtc ggaaggccga ggacgccgcg aagcgcctcg
     5281 cgcgcgccgg cgtcgcccgc gacgaagcgg tcgcgcgacg ggagcgccgc cgtcggcgag
     5341 agcgccgcga ggcggtcgaa gaccgccgcg cgccccgcgg cgtcgaggcg gggcgcgagg
     5401 ccgaggagcg tgaggccgtc ccgcggccgc gccgtgcgga ggaggtcgtc gagcggcggg
     5461 gcgtcgccgg cgctctccgc gctctcctcc caccgcgcga gggcggagag gaacggctcc
     5521 ggagcgtcgt cccaggccgg cacgcccggt ccccgccgcg ccgagacacg gcacgaggcg
     5581 ccggccggga cgacgacctc gtcgagcgcg ccggccgtcc gcctctcgaa cgagacccac
     5641 ccggcgcgga cccggaggag cccgttcccg gcctcgtcgg ttgagagggt gtaggcacag
     5701 ccgaggtcga tcgcggtcgc ggcgggggtc tcgacgaaga agcgccgcgg cggcgcctgg
     5761 atctccgcgc ggacggtgcc gcgggcgagc gccacgcggt ggtcgcgggg agagtcggag
     5821 acgaggcgga ggcgcgtccc gggctcgagc gtgagcgtcc cgatgcgccc caccgagagc
     5881 cgggccgtcg cgccgcccgt ctcgagccac tctccgcgcg cgaggcgcgt cgtccgctcc
     5941 ccctcccagc gcacctccca gcccttgccg cgcgcgagga gcgcgaggga ggcggcgacg
     6001 aggaggcacg ccgccaggag ggccggcgcc gcgacgaaga ggcggcgcca ggagggcggg
     6061 aggacgggcg agcggtccgg gagctcccgg agggacggcg gctcgcgccg cagcggggcg
     6121 aggcgctcct cgatcgccct cacgtcggga tcaggctcgt gcgcggggtt ccagaggtag
     6181 tcgctcatcg gcgtcctccg agcagcccgc ggagcttcgc gaagccgcgg tggaggttca
     6241 cgcggaccga gccttccgtc agccccgtct cgcgcgcgat ctcggggccg ctcatcccct
     6301 cgaggaggcg aaggacgagc ggctctcggt aggcctccgg gaggaggcgg atggcggcga
     6361 ggaccgcgcc gggcccctcg tcggcgcggg cgcccccggg gaggtcgtcg gggagcggcg
     6421 cgtggcgggg ggccgagcgg acgtggctcg ccgcccggtt ccgcgcgatc gccgcgaccc
     6481 acccgccgaa gccctccggg tcgcggaggt cgccgagctt ctcccacgcc gccaggaaga
     6541 cctcctgcgc gaggtcttcg gcgtcggggc gcgggaggcg cgcgaggagg accgcgcgga
     6601 cgaggcgcca gtagcgccgg tagagctcgt cgaaagcgtc gcggtccccg tcgcgcgccg
     6661 cggcgacgag ctccgcgccg ctcgcggccg gtccgggcgc ggcgagcctc agccccacgg
     6721 cggacccctc cgcgaacgcc gcgctctcct ccatcgtgct gaaaggacgc gcgccggcgc
     6781 cggaccgtta gcagccctgc tcacaccacc gctacgttaa cagcgccccc cgcggccgcg
     6841 tcctccccca cgaaccgcag agcacgcgaa cggaggccgc ccatgaacga ccgccccgtc
     6901 ccgacccgcc gccgcttcct cgccctcggc ctcggcgccg ccgccgcggc gtggggaggc
     6961 gccctcgccg cctcccgcga cgacgacccg tgcctcgtcg cccgccccga ggcgcgcctc
     7021 gcgccgccgg gcgagccggg ctcgccgctc cgcgtcgaag gtctcgtcct cgctcccgac
     7081 gggacgaccc ccgccgccgg cgtcgtcgtc tacgcctacc agaccgacgc gaccggcgtc
     7141 tacgcgcccc cgggccggcc tccgcggctg aagggctgga tgaggacgga ctccgacggc
     7201 cgcttcgcgt acgacacgat ccgccccgcg ccctaccccg gcggacggaa ccccgcccac
     7261 gtccaccacc agctctgggg cgccggctgg ccggcccagt gggggaacga cctcctcttc
     7321 gacgacgacc cgctcgtccc cgagtccgag cggcgccgct ccgcctccct cggccccttc
     7381 gcgttcgtgc gcgccgtcga ggagcacccg ctccacggcg cgctcgtgaa gctgcgcctc
     7441 cggctgagga cggcgggcga ccgcttcgag gacgggactc gccacggcgt cgacgcctgc
     7501 cggcatgggc gaggcggccg gtgagggcca ccctcgcgct cgcggcggcc cggacggtcg
     7561 aggttcgcct tctcgtcgcg gcctcggcgc ggccgccccg ctgacgccag gcccggtcga
     7621 caccccctcg cgcttcgggc acgatccgcg ccgaggaggc ccccgtgccc gaaacgatca
     7681 ccgcgctcga gctctccgcc ctcgtctccc gcgtcttcca gccgaccgcg gaggacacgc
     7741 gcctcgggat catggtcgac ctccctgacg agaaggtcct cgacgacgcc ccctgggcgg
     7801 cccgccgcgc gatcgccgcc gagtgggcgg agctcctcgg gatgcaggtc cgcgaggccg
     7861 ggtacgaaac gcgcctcctc ctctacccga acgtccacac gaacaacggc gaccttccag
     7921 gccgctgctg gatccatgcg ggcggccccg tcccggcgag cgccgcggag ctcgacccgg
     7981 cggcctcggt cgcgatgacg gagatcttcg acacgttccc gatgctcctc gcgctgacga
     8041 agttcagctc gacggcgccg ctgaagatgg ccgcgaagaa gcacccgatg cgcgccgcga
     8101 cgatgcccgg cttctccgcc gcgatgatcc cggccctccg gctcgactac ggcgaggtga
     8161 accgccgcgt cgacatcctg aaggcgcacc tcgaccgcgc gattggcgcc gacttcgtct
     8221 tccgccgccc ggccggcgag gatcgccttc acctcgacct gcgtcaccgg acgggccacg
     8281 cttccggcgg cctcctcccg aagcgcggcg tcgccgggaa cctcccctcg ggcgagtcgt
     8341 acatcgtccc ctacgaggga gaggtcgaag gcgacccgag ccgttcggag ggcgtcctcc
     8401 cggtccagtt cggcaaggag gtcgtccgct accgcgtcga gcgcaaccgc gccgtcgagg
     8461 tcctcactcg cggccccgag tcggagcggg aggcggcgct cctcgcgaag gagcccgcct
     8521 acgcgaacct cgccgagctc ggcctcggcg tcctcgcggc gttcggcgtc aagccggtcg
     8581 gctccgtcct cctcgacgag aagctcggcc tccacatcgc gttcgggagg agcgagcact
     8641 tcggcggcca ggtcggcccc gacgccttct cctcgcccga ggcggtcgtc cacatcgacc
     8701 gcgtctacgt ccccgagacc cagcccgacg tcctcccggc gatcgtcgac ctcgtcttcg
     8761 aaggcgggga gcgcctcccc ctcatgcgcg acggggagtt cgggccggtg ttctgacccg
     8821 gccgggcggc ggcgattgct tccccgggcg acgggtctcg cctatcctcc gcccgctacc
     8881 cgaggtaccg ccgaaaggag aactcccatg agctctcgtg cagggactgc tctcgccgcc
     8941 tgcgctctcg ttctctccct cgtcgccccc gcggcgcccc tgtcctccgt cacgtgcaat
     9001 ggcgtggaga tgccggtgaa ggccggagtc gcggtctggg acgcgaaggg gacgacgctc
     9061 cagctcttcc tgctcccgtt cgagccgacg gcggccgaaa cggagcggct cggccgcggg
     9121 gacacgacct ggctggacaa gcgagccccc gtcgacccga agaagtggcc ccgctggaac
     9181 ccgcgcgccg acctcctcct cagctggtcc tggaacccga aggccgtggg tgacgcctca
     9241 aagctgaaca tgtgcacgct gttccagtac gacatcgcga agaacgccgt caacagcttc
     9301 gtcgactgcc cggaccagaa gctgaacggc cggctcgaag ggcggctcgc cgcgggagag
     9361 tccatcacgg tcgcgtcccg gggcgaaggg aagaacgggg actacgcgct ctcgtgggac
     9421 ttccacgtca ccacgaaggt cctctccaag ccctgaccgc gcgctcgccg ccgggccggc
     9481 tccggccgac ccggcggccg gagcacccgc cctcaggcga ggtcgatcct ccggctgagc
     9541 atccggtcgc cgtcgaagac gaagaaggcc cggaacggct tcccctcgag ggcgagaggg
     9601 agccagtagc ggtcgtcctc ccacatccgc tcgtacggga tctcaccgac cgggcaccag
     9661 aacgggtcgg cctcgcgcgt ctcgcgcggc gtgccggagt gcgaggcggc gaagaagacc
     9721 gtcccgtgga gcgcgtagcc gtcgacgaac tggaacgaga gctcgcccgc cttcgtcagg
     9781 cgggacgctt cgacgccgac ctcttcgcgc acctcgcgga tcgcggcctc ctcggccgtc
     9841 tcgcccgggt cgatccgtcc gcccggcgcg ttcaccttcc cggcgccgag gccggtcttc
     9901 ttgtggatca ggaggaggag gtcgccgtcc ctgacgaagc agaggacggc gtgctcggtc
     9961 ggctcccagc gcgaccagtc gacctccgcg aggccgcccg ggcggtcgga aggatcagag
    10021 ccggccggca aggagcgtcc cctgctcgat cgcgcgcttc gcgtcgagct cggccgcgac
    10081 gtcggcgccc ccgatgagat gcacggagac acccgcctcg cggagcggcg cctccagctc
    10141 gcggagcggc tcctgccccg cgcagaggac gacggtgtcg acctcgagga ggcgcgtctc
    10201 ggggctcttc gtccctcgcg tgatcgtcag cccagcgtcg tcgatccgct cgtagcggac
    10261 gccggcgagc atctcgaccc ccttcgcctt gagcgcggcg cggtggatcc accccgtcgt
    10321 cttcgcgagg ccggcccccg gcttcgtggc cttgcgctgg agcagcgtga cgcggcgcgc
    10381 cgccttcgcc gggcgcggct tcacgagccc gccgcgcgcc gtctccgggt cggcgacgcc
    10441 ccactccgcc ttccaccgct cgaggtcgcg gccgtgcccc tcggctccct ccaccaggaa
    10501 cgtcgcgacg tcgaagccga tcccgcccgc gccgacgatc gccacccgct cgccgacgct
    10561 cttccgcccg aggaggacgt cgacgtacga gacgaccttc gggtgctcct gccccgggat
    10621 gccggggctc cgcgggacga cccccgtcgc gaggacgacg tcgtcgtact tccctgcgac
    10681 gagcgcctcg gccgtcgcac gcgtcccgcg cttcaggctc acgcccgtcg tctcgatccg
    10741 ccgcgcgaag tagcgaagcg tctcggcgaa ctcctccttc ccggggacgc gcttcgccag
    10801 gttgaactgc ccgccgatct cccccaccgc ctcgaagagg tcgacggcgt gcccgcgcgc
    10861 ggcgagctcc gtcgcgcagg cgaggcccgc cgggccggcg ccgacgacgg cgatgcgctt
    10921 gcgcgccgcg gccacgggga agacgacgac ggtctcgcgg caggcccgcg ggttgacgag
    10981 gcaggagacc tccctcccgt cgaagacgtg gtcgaggcag gcctggttgc aggcgatgca
    11041 ggtgttgatc tcgtccgcgc gcccctccgc cgccttccgg acgaagtcgg ggtcggcgag
    11101 gagcggccgg gccatcgaga ccatgtcggc gccgcccgag gcgaggatct cctcggcgac
    11161 ctccggcgcg ttgatccggt tcgtcgtgca gaggggaatg ccgacctctc ccttcagctt
    11221 ccgcgtgatc caggcgaacg cggcgcgcgg cacgctcgtc gcgatcgtcg ggacgcgcgc
    11281 ctcgtgccag ccgatccccg tgttcaggat cgtcgccccc gcccgctcga ccgccttcgc
    11341 gagcgcgacg acctcctccc agctgctccc gtcggggacg aggtcgagca tcgagatccg
    11401 gaagacgagg atgaagtcct tgccgaccgc ctcgcgcacg cgcgagacga cctccaccgg
    11461 gaggcgcatc cggttctcgt acggcccgcc ccaccggtcg gtcctcttgt tcgtgcgggt
    11521 gacgaggaac tcgttgagga agtacccctc cgaccccatc acctcgacgc cgtcgtaccc
    11581 cgcctcgcgg gcgaggacgc cgcacctcac gaaggcccgg atctgccgct cgacgccgcg
    11641 cgccgagagc gcccgcggcg tgaacggcga gatcggcgac ttcacccgcg acggggcgac
    11701 ggcgagcggc gtgtagccgt accgccccga gtggaggatc tggagcgcga tcttcccccc
    11761 ctcggcgtgg accgcgtcgg tgatcgggcg gtgggcccgc gcggcccgcg cgctcgcgag
    11821 gcgggagccg aacggcgaga gccacccctc gacgttcggg gcgaagccgc ccgtcacgat
    11881 cagccccgcc ccgccgcgcg cccgcgccgc gaagtaggcc gcgagccgcg ggaagcggct
    11941 cgtccggtcc tccagccccg tgtgcatcga gcccatcagg acccggttcc ggagcgtcgt
    12001 gaacccgagg tcgagcggcg cgaggaggtg aggatacgga gcgggccggc tcatggacgc
    12061 cgagtatccc gcgagagggg agggcgcggg gtcgccgttc ggcaccgccc ccgcccccct
    12121 actcgatccc gtagtcgacc tcggtccgcc gcaccttcca ggccgcgaac gcgaggagcg
    12181 ccgcggtgaa gaggagcgga acggggatcg agacccagac ggggagcggc tcgccgagga
    12241 tcgcgaggaa gctctcgggg acccggaccg gcgtcagcga gaggatgtag tggatgaggc
    12301 cgagccgctt gagcccctcg gggaggagga agttcaggtg ctcccacccc cagaacgcga
    12361 gcgccgggaa gatcgggttc ttcacccact ggccgaacgc gaggaagacg gcgccgtagg
    12421 ccgcgcaggc gaggaccgcg acgaagacgt aggcggccgt tcgccccgcc ccgcccgcgc
    12481 cgaggtgagc gagcattgcc ggccgccccg agtccgagag gaggaggaga taggaggcgg
    12541 cggaggtgag gccgaagagc gtcgcggtga cggcgcaggc ggcgaggtac ttgccgagga
    12601 ggaccacctc gcggcggaca ggggcgagga ggaggtggtg gagcgtcttc gcgatctgct
    12661 cgccgcggac gaggccgccg aacgtgaaga ggcacccgaa gtagagcgtc ccctgcagga
    12721 cgaggttctg gaagatgaag gcgaacgcgt tctcggctcg tccggccgtg aggggcgtgt
    12781 tcctcgccgt gaggccgacg atcgcgagga agacgggcgc cagcgcgaag aagagcgtcg
    12841 cccgcccttc ccgtccgcgg aagaggcgct tcagctcgag gacgaacacc gcgcggagct
    12901 gcctgagcct cacgtcccgg ctcattcccc ctcccctccc tcgatcaggt agcggtagac
    12961 cgccttcacg tcgtcgtcgg cgggcgtcac cgtctcgacc tcgagaccgt cggtgaggac
    13021 ggcgtcgttc agggcgagga agaagcggtc gggcgtccgg gtccgcgcca gcaggccgcc
    13081 gccgtcctcg tggagctcga cctcggtcac gagcccctcg gtgaagaggc gcgccgcgag
    13141 ggcgccgggc cggtcgcagc ggacgaggac ctgcatcggc cgctcgacga cctcctcgcg
    13201 caggccgtgc gactcccctt ccgcgacgac gtagcccgac ttcacgagga cgacgcggtc
    13261 ggcgaggagg tccacctcgt ggaggatgtg gctcgagacg acgacgtgct tcccctcggc
    13321 cgcgagggtc cggaaaaggt ccaggacctc cgcccgggcc atcgggtcga gtccgttcaa
    13381 cggctcgtcg aggaggagga cgcgcgggtc gggcgcgatc gccgccgcga ggcggagcct
    13441 ctgcttcat
//
LOCUS       DAOTEZ010000065        13414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3203_length_13414_cov_6.880680, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000065 DAOTEZ010000000
VERSION     DAOTEZ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13414
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3203_length_13414_cov_6.880680"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3116)
                     /gene="dnaE"
                     /locus_tag="P5164_05210"
     CDS             complement(<1..3116)
                     /gene="dnaE"
                     /locus_tag="P5164_05210"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRY43328.1"
                     /translation="MGEIGPPARDMFMPGDAKKIVSPGARKEELRKRRFGGPAAAPKV
                     RWAELRCASAFSFLEGASEPETLVERAAALGLPAVALVDRNGVSGAPRFFKAAKAAGL
                     KAIVGAEVVLDEAALPKDLRGPLGLVPPPPPPGLPPARVTLLAESRTGYRNLSRLLTA
                     AALGKPKGQAAASWADVAAHAEGLHVLSGGIEGPVLRALERVGADAAGKLLQRLAAIF
                     PGRLHVELQRHRVRDEEARNAVLLELARAQRLPLVATNGVRYARRRDKDLFDVLTCIR
                     NHTTVDSAGRLLEAGSERHLKGPEEMAALFADLPGAVGGAADLADRLAFTLADLGYRF
                     PDAKVPPGETPSSWLRQKTWEGARSRFQPLTAKAQDQLKKELDLIEKLDLAGYFLVVH
                     DVVEFCRRENVLVQGRGSAANSAVCYALSITAVDPVKMELLFERFLSEERGEWPDIDL
                     DLPSGDAREKVIQHVYATYGVHGAAMTANCITYRDRSAARETAKALGFSTEQVDALAK
                     RLATWSFGEYRDAAGSLTARETGDGDPKVAAAARAEAFAEGTAAPPSLPPKMAEEMRA
                     AGFDPDDVRLRHFGSLFRRIQNLPRHLGQHSGGMVVAGGRLDEVVPLEPAAMPGRVVI
                     QWDKDDCADLGIIKIDLLGLGMLGAIAEMVPTIRSAEGVHVDLAHLPHDDPAVYEILN
                     KADTVGLFQVESRAQMASLPRNAPRCFYDLVVQVAIIRPGPIQGGMVHPFFDRRQGRI
                     PVEYPHPSLEPILKRTLGVPLFQEQLLKIAMVAAGFSGGEAEELRRAMGFKRSSERMA
                     VIEGRLRAGMAERGIVGEAQEQIVRSITSFALYGFPESHAASFALIAWASGWLKVHHP
                     AAFLAGLLNAWPMGFYHPATLVKDSQHHGTEVLPIDVTRSGRLCRVERLRGPWPKHLP
                     RPSPAVVTPSLAVRLGMRYVRGLRASTADAIEREAARAPFDGVKDLARRASLREDELS
                     ALAHAGAFATLPDAATGERGLSRRTALWQAASAARDMGPLYDTLEDDVPSPLPEMSRF
                     EETVSDYQT"
     gene            3364..5481
                     /gene="tssI"
                     /locus_tag="P5164_05215"
     CDS             3364..5481
                     /gene="tssI"
                     /locus_tag="P5164_05215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HRY43329.1"
                     /translation="MGEYLQQQRKLRVTTPLGENELLLKGFKGEEGISRLFSYRLEMI
                     AENKTKVAFDAVLGQKVTVHLQLPDESETHLNGLCVRLAQGERDEKFTLYEAELVPDV
                     WRLTRKAQSRIFQRMTVPEILKKVFTGFDADWQLTGKYEPRDYCAQYRETDFSFGSRL
                     MEEEGIYYFFEHSDGSHKMIVADNPLEHPKLAGENRIPYEEVEGGTREENRIWAWKKE
                     QEIRPGKYVLWDHSFELPHKHLEAETLTLDSVPVGKETHKLKVAGVDQMEVYDYPGEY
                     AQRFDGITAGGGDQASELQKIFRDNQRTVELRMDEETTPAVVLRGRSNVKHLLSGYGF
                     TLQRHFSGDGDYVLTSVRHEATYGADYRSGQDVTMTYENTFTCIPAALPYRPERKTAK
                     PVVQGSQTAVVVGPSGEEIFTDKYGRVKVQFHWDREGKNDENSSCWVRVATSWAGRNW
                     GAIRIPRIGQEVVVGFLEGDPDRPIVVGSVYNADMMPPWDLPANKTRSGVKSRSSKGG
                     GPANFNEIMFEDKKGGELLKIHAERNQSISVEADETHTVGNDRTKSVSNNETTSIGNN
                     RSETVTNNEMITVGVARTIMVGGAETRTIGGAETIAVGGVYSLAIGGACSTTVGGSST
                     TNVGSDASTQVGGSAATSASKSVSITAGNAVQIQSGKAGITLKKDGTIVIKGKDITLD
                     ASGKINIKAASSVTIKGSKIQEN"
     gene            5516..6517
                     /locus_tag="P5164_05220"
     CDS             5516..6517
                     /locus_tag="P5164_05220"
                     /inference="COORDINATES: protein motif:HMM:NF021456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HRY43330.1"
                     /translation="MDLVNRSPFAAERALVLDEAGAETLAVLLKASFAWTPRTGLAPA
                     AEQVPVRFADEHLGEPGRSSILRASDLCPGKPGCDLVLLGSAGRSRPAPQVDVSFRVG
                     AWGRELRVFGERRWSGSAGVPDASPFEALELTWENAFGGRDEDVPAGEPWEEELRNPV
                     GRGFRARKSRRPVSGDLLPSIEDPADPVRSPLDRPAPAGFGFVAPHWEPRRSRAGTYD
                     EAWQRDRAPLLPEDFDPRSRQRGAPGLVAPEAPAAGAPVVVTGVRPHGEVRFALPRVV
                     PSGAVYFRTRKSDLSFALQTVLFDANASLLELTFGAYQSVHGQTRDVECVAVEAEAP"
     gene            6514..7638
                     /locus_tag="P5164_05225"
     CDS             6514..7638
                     /locus_tag="P5164_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRY43331.1"
                     /translation="MSAAAPRPGAGGGIAITGLGAVTAVGATLDQTATSVSAGVRRMS
                     ERPLAGEGGEPPDEGGVYVAASAEALAASLGSAAPVDLALAAAREALWDAGLYEPGDV
                     DAAYRRNAVAVSLALPYADAPGASEADADAFVEGAVAAGLLAGEPAVAIVPCRNGHAG
                     ALLALEAASARLRAGEIDVALVGGLDATLDAARIAELNRLSKLRTDRAPGGAIPGEGS
                     AFLVVERLADARRRSARFAAAVESVARDTEPVPLDGTRPGRADGATRAVARALGAAPS
                     ARRIEEIWCDLNGERGRALEWGFLETRALAALGAARRRAPAAFVGDLGSASGALLLAL
                     AAYALRAPRGAGGAALVCSASERGERAAAVLAAPRAGGED"
     gene            7644..9110
                     /locus_tag="P5164_05230"
     CDS             7644..9110
                     /locus_tag="P5164_05230"
                     /inference="COORDINATES: protein motif:HMM:NF025047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4150 domain-containing protein"
                     /protein_id="HRY43332.1"
                     /translation="MPTTTVNMLTVVHKKSGGVSTAFPDICKTPTPAGGIPAPIPYPN
                     IAFSKDTANGSTTVKVDGQSIMLLKSNFSTSTGDEPGSVGGIISQIIKGKAQPITASF
                     DVMVDGDFVFRLFDLMLQNANPPPNTPPGVLIQAPLTPYIPWLNPPKWQVKWSKGEAK
                     CGDEVDILVQAMGGDPGYYLSMTVSRAPHDLPVTYFVTAISGGSAKIPWVTLTGPSPK
                     NKVQLDIYAFGAGEYAKSSSPLKIVVPEETRQAGSKKNRTVPQMTPVLQPDGTYTYTQ
                     GTGLWGWDYGYDLEIKDGILKVTGRIKLVARGGAVFGARHKRGWKAEIEAIWTRKWRE
                     HRKKCKRGKDCKCWGGCCLFPVVFECLFVGSGEFAEVEVWAGNPDTYGTDASGAHNKW
                     WNSGTWFMERCGHEGNQAGVRAHEFGHAIGLYDEYRTGAIFVPTDASGTVTGPPPRPN
                     VPGSLMGPNGLGIKRNHLDEFHQWFLGKANDEYTRERL"
     gene            9164..10123
                     /locus_tag="P5164_05235"
     CDS             9164..10123
                     /locus_tag="P5164_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43333.1"
                     /translation="MDGALGRSGLRAASVLFAAALACCAAPPAPAAGKAFSPMAESWV
                     FFTRSVGSAGTLGGSFTADAALVRTDASLPGIPRIGFFRRPAEAGEFSRLAGSIRALG
                     APRRSGTHFPGAPLVSLGIMTNGKAEVVHGQVESALTPAERQVFSSLEATVEKVLDHP
                     HKALEASIEWVRGTIEPDDELAILVRVSNPGLVPVPFVHPAGGEEGESALAVVLARVG
                     PDGVPVEARDITFAASEVTETGPDGKPVAGPYPAIVELAPGKDVHLRARSRVRLVPAS
                     YRASARIDVEAPPDSDEKAVSGTISVELPPLGVVRPGAKRGKD"
     gene            10123..10569
                     /locus_tag="P5164_05240"
     CDS             10123..10569
                     /locus_tag="P5164_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43334.1"
                     /translation="MPDTKKLLDVVTSAKPGVLFGRVSALLDDAVLVSCGEGSELVAC
                     DLLDPGAGRPGALAEGERVLVVVPEGGGGRGVVLGRVASPAALAARPKETPDELLLEA
                     KKGLTLRVGEGSITLREDGKVLIRGRDLVSHARRLNRIRGGSVSIN"
     gene            10580..11803
                     /locus_tag="P5164_05245"
     CDS             10580..11803
                     /locus_tag="P5164_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43335.1"
                     /translation="MISPKVVLLRDVLEEHLEELPFLWQLRRDAVRSPERTRSDVDRL
                     DERVRAHADGLLVARGELGEVVGPLLMSDDPPAAFAAAFVLLRTGEEGARAVLDALRA
                     AGPGGREGLAEALAFGETRYAREELASLAGSDDPSLAVAAATALALGRGAAAPDVTRL
                     LGESDPAVRAAAWRLVPFQARAPRPELLRKGLDDPDPGVVREAAEAAAWFRSPLALEA
                     SRERSAGTGEGAGEWLRLLAVLAEEGDVPGVLAGSAKGTAAGRGASGPRALGALGHPR
                     AVELLIEAMGDEDPARAAAAGAAFRKVTGFDASGESRVALRPDGPPPDDFDEEFLDEV
                     TLPDAPQARAFWASARGKLAGGKRWCYGVDVGAGVPAALPAAFDMESCFEVRLRGRFT
                     GTFTGDAGELLRMAP"
     gene            complement(11819..13297)
                     /locus_tag="P5164_05250"
     CDS             complement(11819..13297)
                     /locus_tag="P5164_05250"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43336.1"
                     /translation="MTLVLGVVTAFVQKREIDRGREERMRFLTMTLAPLAADIGDATD
                     LATIQDRLTRFQEAHAASGFGDVRVLLFDSDGRVFATPFNGPAPLPSTDATTARVPVR
                     SGLLPDGNGSLQVSQDETRFRSMARRLWQHWLLTLAVGAGCILVSLYLAYFFLIERPL
                     RGLLEGVRQMEQGYWSGLEIPRGAWEMRWLAYRFRNLGTQLEETVRRLVEAERRTIVG
                     LRAAAPGPPARTAGEGSPAGASMQEEAFRKRILRRYLVARCRFLETRSSSDPAARAVA
                     HEVWERDVHEAQRMGESALKSRLEDAAFRILEPDAYEDVSRRAAGLTSSRRKWIRDRE
                     AELKSALTEAGVKPRALQHRVKHLAGIWRKMKSKGLAIEQIHDIFAFRILLETEEECY
                     LALSALHHRFEPLLLRFKDYIAEPKENGYQSLHTCIRAPGGLVYEVQIRTVAMHEAAE
                     GGGAAHWKYKKESVEIDGGEDELPQGVLVSLKDALRRRGIGS"
BASE COUNT         1893 a         4649 c         5097 g         1775 t
ORIGIN      
        1 gtctggtagt cggagacggt ctcctcgaag cggctcatct cggggagggg cgaggggacg
       61 tcgtcctcga gcgtgtcgta aaggggcccc atgtcgcgcg cggcggaggc ggcctgccag
      121 agggccgtcc ggcgcgaaag cccgcgctcg ccggtggcgg cgtcggggag cgtcgcgaag
      181 gcgccggcgt gggcgagggc ggagagctcg tcctcgcgga gtgaggccct gcgggcgagg
      241 tccttgacgc cgtcgaacgg cgcccgcgcg gcctcgcgct cgatggcgtc ggccgttgac
      301 gcgcgcaggc cgcgcacgta gcgcatcccg aggcggacgg cgagggaggg cgtgacgacg
      361 gcgggagacg ggcgcgggag gtgcttcggc cacgggccgc ggagccgctc gacacggcag
      421 aggcggcccg agcgggtcac gtcgatcggc aggacctccg tcccgtggtg ctgagagtcc
      481 ttcacgagcg tcgcggggtg gtagaagccc atcggccagg cgttgaggag gccggcgagg
      541 aacgcggcgg ggtggtggac cttcagccag ccgctcgccc aggcgatgag ggcgaagctc
      601 gccgcgtgcg actcggggaa gccgtagagc gcgaacgacg tgatcgaccg gacgatctgc
      661 tcctgcgcct cgccgacgat gccgcgctcc gccatcccgg cgcggaggcg cccctcgatc
      721 accgccatcc gttcgctcga gcgcttgaag cccatcgcgc ggcgcagctc ctcggcctcg
      781 ccgccgctga agccggccgc gaccatggcg atcttcagga gctgctcctg gaagagcggg
      841 acgccgaggg tgcgcttcag gatcggctcg agcgaggggt gcgggtactc gaccgggatg
      901 cgcccctgcc gccggtcgaa gaaggggtgg accatgccgc cctggatcgg ccccggccgg
      961 atgatcgcga cctggacgac gaggtcgtag aagcagcggg gggcgttccg cgggagcgag
     1021 gccatctgcg cgcggctctc gacctggaag agccccacgg tgtcggcctt gttgaggatc
     1081 tcgtagacgg ccgggtcgtc gtgcgggagg tgcgcgaggt cgacgtggac cccttcggcg
     1141 ctgcggatcg tcgggaccat ctcggcgatg gcgccgagca tgccgaggcc gaggaggtcg
     1201 atcttgatga tcccgaggtc ggcgcagtcg tccttgtccc actggatgac gacgcgcccc
     1261 ggcatcgcag cgggctcgag cggaacgacc tcgtcgaggc gcccccccgc gacgaccatc
     1321 ccccccgagt gctgcccgag gtgccgcggg aggttctgga tgcgccggaa gagggagccg
     1381 aaatggcgga ggcggacgtc gtcggggtcg aagccggcgg cgcgcatctc ctctgccatc
     1441 ttcgggggga gcgacggggg cgccgccgtc ccttcggcga acgcctcggc ccgcgccgcg
     1501 gccgcaacct tcgggtcgcc gtcgcccgtc tcccgggccg tgagcgaccc ggcggcatcg
     1561 cggtattccc cgaagctcca cgtcgcgagc ctcttggcga gcgcgtcaac ctgctcggtc
     1621 gagaagccga gcgccttggc cgtctcgcgc gcggcggagc ggtcgcggta ggtgatgcag
     1681 ttcgccgtca tcgcggcgcc gtggacgccg tacgtcgcgt agacgtgctg gatcaccttc
     1741 tcccgcgcgt cgcccgaggg gaggtcgagg tcgatgtcgg gccactcgcc ccgctcctcc
     1801 gagaggaagc gctcgaagag gagctccatc ttcaccgggt cgacggcggt gatcgagagg
     1861 gcgtagcaga cggcgctgtt cgcggccgat ccgcgcccct ggacgaggac gttctccctc
     1921 ctgcagaact cgacgacgtc gtggacgacg aggaagtagc ccgcgaggtc gagcttctcg
     1981 atcaggtcga gctccttctt cagctggtcc tgcgccttcg ccgtcagcgg ctggaagcgc
     2041 gaccgcgccc cttcccacgt cttctgtctc agccacgacg agggcgtctc tcccggcggc
     2101 accttcgcgt cggggaagcg gtagccgagg tcggcgagcg tgaacgcgag gcggtccgcg
     2161 aggtcggccg cgccgccgac ggccccgggg aggtcggcga agagggcggc catctcctcg
     2221 ggccccttga ggtgccgctc cgagcccgcc tcgaggaggc gccccgccga atcgaccgtc
     2281 gtgtggttcc ggatgcaggt gaggacgtcg aagaggtcct tgtcgcggcg gcgcgcgtag
     2341 cggacgccgt tcgtcgcgac gagcgggagg cgctgcgcgc gggcgagctc gaggaggacg
     2401 gcgttcctcg cctcctcgtc gcgcacgcgg tgccgctgca gctcgacgtg gaggcggccc
     2461 gggaagatcg ccgcgaggcg ctgaaggagc ttgcccgccg cgtcggcccc gacccgctcg
     2521 agggcgcgga ggacgggccc ctcgatccct cccgagagga cgtggagccc ctcggcgtgc
     2581 gcggccacgt cggcccacga ggccgcggcc tggcccttgg gcttgccgag cgctgcggcg
     2641 gtcaggaggc ggctgaggtt gcgatagccc gttcggctct cggcgaggag cgtgacgcgc
     2701 gcgggcggca ggcccggcgg gggcgggggc gggacgaggc cgagcggccc gcggagatcc
     2761 ttcgggaggg ccgcctcgtc gaggacgacc tcggctccga cgatcgcctt caggcccgcg
     2821 gccttcgccg ccttgaagaa gcggggcgcg ccggagacgc cgttccggtc gacgagggcg
     2881 acggcgggaa ggccgagcgc ggcggcgcgc tcgacgagcg tctcgggctc ggaggccccc
     2941 tcgaggaaag agaaggccga cgcgcagcgc agctcggccc agcggacctt cggtgcggcg
     3001 gcggggccgc cgaagcggcg tttacgcagc tcctccttcc gcgcgccggg ggagacgatc
     3061 ttcttcgcgt cccccggcat gaacatgtcg cgggcggggg ggccgatttc gcccatgggc
     3121 gtaaattaga cgcaagtcat acgggaatca agcgccgtct ccgcagggcg ctgcccgccg
     3181 acggtggcga cgacgcggcg aacgaggaag cgcacgccgc gcgcgttccg gtgagccggc
     3241 tcacggcgat gccctttggt cccgccgttt ctttgacccg acctctcccg gaccctatat
     3301 tcccgcggca agcagcaacg gatcagctct cgaatccggg tgaagcgccc gggaggacgg
     3361 acgatgggcg agtatctcca gcagcagcgc aagctccggg tgacgacgcc gctcggcgag
     3421 aacgagctcc tcctgaaggg cttcaagggg gaggagggga tctcccggct cttctcgtac
     3481 cgcctggaga tgatcgcgga gaacaagacg aaggtcgcgt tcgacgcggt gctggggcag
     3541 aaggtgacgg tccacctgca gctgccggac gagagcgaga cccacctgaa cgggctctgc
     3601 gtgaggctgg cccaggggga gcgggacgag aagttcacgc tctacgaggc ggagctcgtc
     3661 cccgacgtct ggaggctgac ccggaaggcg cagagccgga tcttccagcg gatgacggtt
     3721 ccggagatcc tgaagaaggt cttcaccggc ttcgacgcgg actggcagct gaccgggaag
     3781 tacgagccgc gcgactactg cgcgcagtac cgggagacgg acttcagctt cgggtcgcgc
     3841 ctgatggaag aggaggggat ctactacttc ttcgagcact ccgacgggag ccacaagatg
     3901 atcgtggcgg acaacccgct cgagcacccg aagctcgcgg gcgagaaccg gatcccgtac
     3961 gaggaggtcg aaggagggac gcgcgaggag aaccggatct gggcctggaa gaaggagcag
     4021 gagatccggc ccggcaagta cgtcctctgg gaccacagct tcgagcttcc gcacaagcac
     4081 ctggaggccg agacgctcac gctcgacagc gtcccggtgg ggaaggagac gcacaagctg
     4141 aaggtcgcgg gcgtcgacca gatggaggtc tacgactacc cgggcgagta cgcgcagcgg
     4201 ttcgacggga tcaccgcggg cggcggcgac caggcgtcgg agctccagaa gatcttccgc
     4261 gacaaccagc ggacggtcga gctgcggatg gacgaggaga cgacgccggc cgtcgtcctc
     4321 cggggccgga gcaacgtgaa gcacctcctg tcggggtacg ggttcacgct gcagaggcac
     4381 ttcagcgggg acggcgacta cgtcctgacg agcgtccggc acgaggcgac gtacggcgcc
     4441 gactaccggt cggggcagga cgtgacgatg acgtacgaga acacgttcac ctgcatcccc
     4501 gcggcgctgc cgtaccggcc ggagcggaag acggcgaagc cggtcgtcca ggggagccag
     4561 acggcggtgg tcgtcgggcc gtcgggcgag gagatcttca ccgacaagta cggccgggtg
     4621 aaggtgcagt tccactggga ccgggaaggg aagaacgacg agaactcgtc gtgctgggtc
     4681 cgcgtggcga cgagctgggc ggggcggaac tggggcgcga tccggatccc gaggatcggc
     4741 caggaggtcg tcgtcggctt cctggagggc gacccggacc ggccgatcgt cgtcggcagc
     4801 gtctacaacg ccgacatgat gccgccctgg gacctgcccg cgaacaagac ccgcagcggc
     4861 gtgaagtccc ggagctcgaa gggcggcggc cccgcgaact tcaacgagat catgttcgag
     4921 gacaagaagg ggggcgagct cctcaagatc cacgccgagc ggaaccagtc gatctcggtc
     4981 gaggcggacg agacccacac cgtcggcaac gaccggacga agtccgtctc caacaacgag
     5041 acgacgagca tcggcaacaa ccggagcgag acggtcacga acaacgagat gatcacggtc
     5101 ggcgtcgccc gcacgatcat ggtcggcggg gccgagaccc ggacgatcgg cggcgccgag
     5161 acgatcgcgg tcgggggcgt ctacagcctc gccatcggcg gggcctgctc cacgaccgtc
     5221 ggcggaagct cgacgacgaa cgtcggctcc gacgcgtcga cgcaggtggg cgggagcgcg
     5281 gcgacgtcgg cgtcgaagtc ggtctcgatc accgcgggga acgccgtgca gatccagtcc
     5341 ggcaaggccg ggatcacgct gaagaaggac ggcacgatcg tcatcaaggg gaaggacatc
     5401 acgctcgacg cgagcgggaa gatcaacatc aaggccgcgt cgtccgtgac gatcaagggc
     5461 tccaagatcc aggagaactg agccgggggc tcggagcgcg aggagcggcg gcacgatgga
     5521 tctcgtcaac cggagcccgt tcgcggcgga gagggcgctc gtcctcgacg aggccggcgc
     5581 ggagacgctc gccgtcctcc tgaaggcgtc gttcgcctgg acgccgcgga ccggcctcgc
     5641 gccggctgcc gagcaggtcc ccgtccgctt cgcggacgag cacctgggcg agcccgggcg
     5701 ctcctcgatc ctccgcgcct cggacctctg ccccggcaag ccggggtgcg acctcgtcct
     5761 cctcggcagc gccgggcgct cgcgccccgc gccgcaggtc gacgtctcgt tccgggtcgg
     5821 ggcgtggggc cgggagctcc gcgtcttcgg cgagcggcgc tggagcgggt cggccggagt
     5881 ccccgacgcc tcgcccttcg aggcgctcga gctgacttgg gagaacgcgt tcggcgggcg
     5941 cgacgaggac gtccctgccg gcgagccctg ggaggaggag ctgcggaacc ccgtggggcg
     6001 cggcttccgc gcccggaagt cgcggaggcc ggtgtcgggc gacctcctgc cgagcatcga
     6061 ggacccggcc gacccggtcc gctctccgct cgaccggccc gcgccggccg gcttcggctt
     6121 cgtggctccg cattgggagc cgcggcgctc gcgggccggg acctacgacg aggcgtggca
     6181 gcgcgatcgc gccccgctcc tgccggaaga cttcgacccg cggagccggc agcgcggagc
     6241 gccggggctc gtcgccccgg aggccccggc ggccggggcg ccggtcgtcg tgacgggcgt
     6301 ccggccccac ggcgaggtcc gcttcgcgct tccgcgggtc gtcccctcgg gggcggtcta
     6361 cttccgaacg cggaagagcg acctctcgtt cgcgctccag accgtcctct tcgacgcgaa
     6421 cgcctcgctc ctcgagctga cgttcggggc ctatcagagc gtccacgggc agacgcgcga
     6481 cgtcgagtgc gtcgcggtcg aggcggaggc gccgtgagcg ccgccgcgcc gcgccccgga
     6541 gccggcggcg ggatcgcgat cacgggcctc ggcgccgtga cggccgtcgg ggcgacgctc
     6601 gaccagaccg cgacgtcggt gagcgcgggc gtgcgccgga tgtcggagcg gccgctcgcc
     6661 ggcgagggag gcgagcctcc ggacgagggc ggcgtctacg tcgcggcctc ggctgaggcg
     6721 ctggcggcgt cgctcgggtc ggccgctccg gtcgatctgg ccctcgccgc cgcccgggag
     6781 gcgctctggg acgccggcct ctacgagccg ggagacgtcg acgcggcgta tcgccgaaac
     6841 gcggtggccg tctccctcgc ccttccgtac gccgacgcgc cgggggcttc cgaggccgac
     6901 gccgacgcgt tcgtcgaggg agcggtcgcg gccgggctcc tcgcgggcga gcccgcggtc
     6961 gcgatcgtcc cctgtcggaa cgggcacgcg ggagccctgc tggcgctcga ggcggcctcg
     7021 gcgcggcttc gggccggaga gatcgacgtg gcgctcgtgg gaggcctcga cgcgacgctc
     7081 gacgcggcgc ggatcgcgga gctgaaccgt ctctcgaagc tccggaccga ccgtgctccg
     7141 ggcggggcga tcccgggcga ggggagcgcg ttcctcgtgg tggagcggct cgcggacgcg
     7201 cggcggcgaa gcgcccgatt cgcagccgcg gtcgagtcgg tcgcccggga caccgagccg
     7261 gtccccctcg acgggacgcg cccgggacgg gccgacgggg cgacgcgtgc ggtcgcgcgc
     7321 gctctcggcg cggcgccctc ggcacgccgg atcgaggaga tctggtgcga cctgaacggc
     7381 gagcgcgggc gggcgctgga gtggggcttc ctcgagacgc gtgcgctcgc ggcgctcggc
     7441 gcggcgcgcc gccgcgctcc ggcggctttc gtcggcgacc tcgggtccgc ctccggcgcg
     7501 ctcctgctgg cgctcgctgc gtacgccctc agggcccctc gcggggccgg cggcgcggcg
     7561 ctcgtctgca gcgcctcgga gcgcggcgag cgggccgcgg cggtcctcgc ggcgccgcgg
     7621 gccggcgggg aggattgacg gcgatgccga cgacgacggt caacatgctg acggtggtcc
     7681 acaagaagag cgggggcgtc tccaccgcct tcccggacat ctgcaagacc ccgacgcccg
     7741 ccggcgggat cccggcgccg atcccctatc cgaacatcgc gttctccaag gacaccgcga
     7801 acgggagcac gacggtgaag gtcgacgggc agtcgatcat gctgctcaag tcgaacttct
     7861 cgacgagcac gggggacgag cccggctccg tcggcggaat catctcccag atcatcaagg
     7921 ggaaagccca gcccatcacc gcttcgttcg acgtcatggt cgacggcgac ttcgtcttcc
     7981 gcctgttcga cctgatgctc cagaacgcaa acccgccgcc gaacacgccc cccggggtcc
     8041 tgatccaggc gccgctgacc ccgtacatcc cctggctgaa cccgccgaag tggcaggtga
     8101 agtggagcaa gggggaggcg aagtgcgggg acgaggtcga catcctcgtc caggcgatgg
     8161 gcggggaccc cggctactac ctcagcatga ccgtctcgcg cgccccgcac gacctccccg
     8221 tgacctactt cgtcacggcg atctccggcg ggagcgcgaa gatcccctgg gtcacgctga
     8281 ccggcccgtc gccgaagaac aaggtccagc tcgacatcta cgccttcggc gcgggcgagt
     8341 acgcgaagtc gtcgagcccg ctgaagatcg tcgttcccga ggagacgcgt caggcggggt
     8401 cgaagaagaa ccggaccgtc ccccagatga cgcccgtcct ccagcccgac ggcacctaca
     8461 cctacacgca ggggaccggc ctctggggct gggactacgg ctacgacctc gagatcaagg
     8521 acggcatcct caaggtgacg gggcggatca agctcgtcgc gcgcggcggc gccgtcttcg
     8581 gcgcccggca caagcggggc tggaaggccg agatcgaggc gatctggacc cggaagtggc
     8641 gcgagcaccg gaagaagtgc aagcgcggca aggactgcaa gtgctggggc ggctgctgcc
     8701 tcttcccggt cgtcttcgag tgccttttcg tcggctcggg agagttcgcg gaggtggagg
     8761 tgtgggcggg gaaccccgac acctacggga ccgatgcctc cggcgcccac aacaagtggt
     8821 ggaactcggg gacctggttc atggagcgct gcggtcacga agggaaccag gcgggcgtga
     8881 gggcccacga gttcgggcac gcgatcggcc tctacgacga gtaccggacc ggggcgatct
     8941 tcgtcccgac cgacgcgagc gggaccgtga cggggccgcc tccccgcccg aacgtcccgg
     9001 ggagcctcat gggaccgaac ggcctcggga tcaagcgcaa ccacctcgac gagttccacc
     9061 agtggttcct cggcaaggcg aacgatgagt acacgcgcga gcgtctctga ccggtctgcg
     9121 ctttccgcgc gcggccgggc gctccggccg cgggaggttc gagatggatg gagcgctggg
     9181 gcgcagcggc cttcgcgcgg cgagcgtcct cttcgccgcg gcgctggcct gctgcgcggc
     9241 gccgccggct ccggcggcgg gaaaggcgtt ctcgccgatg gctgagagct gggtcttctt
     9301 cacgcggagc gtcggctcgg ccgggacgct cggggggagc ttcaccgccg atgcggcgct
     9361 cgtccggacg gacgcctccc tcccgggcat cccccgcatc gggttcttcc gccgtccggc
     9421 ggaggcgggc gagttcagcc gcctcgccgg ctcgatccgg gcgctcggcg ccccgcgccg
     9481 gtccggcacc cacttcccgg gggcgcccct cgtctcgctc ggcatcatga cgaacgggaa
     9541 ggccgaggtc gtccacggac aggtggagtc ggcgctcacc ccggctgaac ggcaggtctt
     9601 ctcgagcctc gaggcgacgg tcgagaaggt cctggaccat ccgcacaagg cgctcgaagc
     9661 gtcgatcgaa tgggttcgcg gaacgatcga gccggacgac gagctcgcga tcctcgtccg
     9721 ggtctcgaac ccggggctcg tgccggtgcc gttcgtccac ccggcgggag gcgaggaggg
     9781 ggagtcggcc ctcgccgtcg tcctcgcgcg cgtcggcccc gacggggtcc ccgtcgaggc
     9841 gcgggacatc acgttcgccg cgagcgaggt gacggagacg gggcccgacg gcaagccggt
     9901 cgccgggccg tatcccgcga tcgtcgagct cgcgcccgga aaggacgtcc acctgcgggc
     9961 gcgttcccgc gtccggctcg tcccggcgag ctaccgcgcg tccgcgagga tcgacgtcga
    10021 ggcgcccccg gactccgacg agaaggccgt ctccgggacg atctcggtcg agctgccgcc
    10081 cctcggggtc gtgcggcccg gcgcgaagag agggaaggac tgatgcccga cacgaagaag
    10141 ctcctcgacg tcgtgacctc ggcgaagccg ggcgtcctct tcggccgcgt ctcggccctc
    10201 ctcgacgacg cagtcctcgt ctcctgcggc gaggggtcgg agctcgtcgc gtgcgacctc
    10261 ctggatcccg gggcggggcg cccgggcgcg ctcgccgagg gcgagcgcgt cctcgtcgtc
    10321 gtgcccgagg gcggcggagg ccggggcgtc gtcctcggcc gggtcgcctc tcccgccgcg
    10381 ctcgcggcgc gcccgaagga gacgccggac gagctcctcc tcgaggcgaa gaaggggctg
    10441 acgctccgcg tgggcgaagg ctccatcacg ctgcgcgagg acggcaaggt cctcatccgc
    10501 ggccgggacc tcgtctccca cgcccggagg ctcaaccgga tccgcggcgg ctccgtctcg
    10561 atcaactgag ggcgacccgg tgatctcgcc gaaggtcgtc ctgctgcgcg acgtcctcga
    10621 ggagcacctc gaggagctcc cgttcctctg gcagctgcgc cgggacgccg tccgctctcc
    10681 ggagcggacc cgctccgacg tggaccggct cgacgagcgc gttcgggccc acgcggacgg
    10741 cctcctcgtc gcccgcggag agctcgggga ggtcgtcggc ccgctcctca tgtccgacga
    10801 cccgcccgcg gcgttcgccg ccgccttcgt cctcctccgc accggggagg agggcgcgcg
    10861 cgccgtcctc gacgcgcttc gcgcggcggg acccggcgga cgggaagggc tcgccgaggc
    10921 gctcgccttc ggagagacgc gatacgcccg ggaggagctg gcctcgctcg cgggctcgga
    10981 cgacccgtcg ctcgccgtcg cggcggcgac cgcgctcgcg ctcgggagag gggcggccgc
    11041 gcccgacgtg acgcggctcc tcggcgaatc cgaccccgcc gtccgcgcgg cggcgtggcg
    11101 gctcgttccg ttccaggccc gcgccccgcg cccggagctc ctgcgcaagg gcctcgacga
    11161 tccggacccg ggcgtcgtgc gcgaagcggc ggaggccgcg gcctggttcc gctcgccgct
    11221 cgcgctcgag gcgagccgcg agcggtcggc cgggacggga gagggcgcgg gcgagtggct
    11281 gcgcctcctc gccgtcctcg ccgaggaggg cgacgtcccg ggcgtcctcg ccggctccgc
    11341 gaagggcacg gcggcaggcc gcggcgcctc ggggccgcgc gccctcgggg cgctcgggca
    11401 tccccgcgcg gtcgagctcc tcatcgaggc gatgggggac gaagacccgg cgcgtgccgc
    11461 ggccgccgga gcggcgttcc ggaaggtgac gggattcgac gcgtcgggcg agagccgggt
    11521 cgcgctccgg cccgacgggc cgccgccgga cgacttcgac gaggagttcc tcgacgaggt
    11581 gacgctcccc gacgcgccgc aggcgcgcgc gttctgggcg tcggctcgcg ggaagctcgc
    11641 cggagggaag aggtggtgct acggggtcga cgtcggcgcg ggcgtccccg cggccctccc
    11701 ggcggcgttc gacatggagt cgtgcttcga ggttcgcctc cggggccgct tcaccgggac
    11761 gttcacggga gacgccggcg agctcctccg gatggcgccg tagcggtgcg ggcgcccttc
    11821 agctcccgat gccgcggcgg cggagggcgt ccttcaggct gacgaggact ccctgcggga
    11881 gctcgtcctc ccctccgtcg atctcgaccg actccttctt gtacttccag tgggcggccc
    11941 cgcccccctc cgcggcctcg tgcatcgcga ccgtgcggat ctgaacctcg tagacgaggc
    12001 cgcccggcgc ccggatgcag gtgtggaggc tctggtagcc gttctccttc ggctcggcga
    12061 tgtagtcctt gaagcggagg aggaggggct cgaagcggtg gtggagcgcc gagagggcca
    12121 ggtagcactc ctcttcggtc tcgaggagga tccggaaggc gaagatgtcg tggatctgct
    12181 cgatcgcgag gcccttcgac ttcatttttc gccagatgcc ggcgaggtgc ttgacgcgat
    12241 gctggagcgc gcggggcttc acccccgcct ccgtgagcgc cgacttcagc tccgcctccc
    12301 ggtcgcggat ccacttgcgg cgggaggagg tgaggcccgc ggcgcgccgg ctcacgtcct
    12361 cgtatgcgtc gggctcgagg atccggaacg cggcgtcctc gagtcgcgac ttcagggcgc
    12421 tctcgcccat tcgctgcgcc tcgtggacgt ccctctccca cacctcgtgc gccacggcgc
    12481 gggccgccgg gtccgaggag ctgcgcgtct ccaggaagcg gcagcgagcc acgaggtatc
    12541 ggcggaggat cctcttgcgg aaggcctcct cctgcatcga ggcgcccgcc ggggagccct
    12601 ctccggccgt gcgggccggc ggcccggggg ccgccgcgcg caggccgacg atcgtccgcc
    12661 gctccgcctc gacgaggcgc cgcacggtct cctcgagctg ggtgccgagg ttgcggaaac
    12721 ggtaggcgag ccagcgcatc tcccaggcgc cgcgcgggat ctccaggccg ctccagtagc
    12781 cctgctccat ctgccggacc ccttcgagga ggccgcggag cgggcgttcg atcaggaaga
    12841 agtacgcgag gtagagggag acgaggatgc accccgcgcc caccgcgagc gtgaggagcc
    12901 agtgctgcca gagccggcgg gccatcgagc ggaaccgggt ctcgtcctgc gagacctgga
    12961 gggacccgtt cccgtcggga aggaggcccg agcggaccgg gacgcgcgcg gtcgtcgcgt
    13021 cggtcgaagg gagcggcgcg gggccgttga agggggtcgc gaagacgcgg ccgtcggagt
    13081 cgaagaggag gacgcggacg tctccgaagc cgctcgcggc gtgcgcttcc tggaaacgcg
    13141 tcagccggtc ctggatcgtg gcgaggtcgg tggcgtcgcc gatgtcggcg gcgaggggcg
    13201 cgagcgtcat cgtcaggaag cgcatccgct cctcgcgccc ccggtcgatc tccctcttct
    13261 gcacgaaggc cgtgacgacc ccgagcacga gggtcacgat gaccgccgtc gtcagtccca
    13321 atcgccagcc gagcggaacg cccctggccg gtacgccgga aaggctcaaa tcgacctcgt
    13381 cacggcaagg accgtgcccc tgattctctc gtca
//
LOCUS       DAOTEZ010000066        13204 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3263_length_13204_cov_6.427029, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000066 DAOTEZ010000000
VERSION     DAOTEZ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13204
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3263_length_13204_cov_6.427029"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2480
                     /locus_tag="P5164_05255"
     CDS             <1..2480
                     /locus_tag="P5164_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRY43337.1"
                     /translation="DGGERGLYRTTDGGKSWTRVLGGPGRTGAIDVVQDPKDPKLLYA
                     ALWERDRKPWNFLESGPGSGVYRSRDGGATWSRLAGGLPQGDVVGRIGLAVTPAKPGA
                     VWAVVDNQARRPGERDEETPPGELTPRRLKALTAEQFAKVETAVVERFLRANGFPKEL
                     TARALQRDVASGKVKLADVVAYVDDADRQMEEVETVGVEVWRSDDAGESWRKAHGVSL
                     EKVAYTYGYYFGKIWVAPDDPERLYVAGVPLAGSSDGGKSWSGLDRHGVHVDHHALWF
                     DPRDPRHVVLGNDGGLNLSWDRGATWTKVSNLPVGQVTTVAVDSAEPYNVLVGMQDNG
                     VWRGPSTYVSGATPPDAWKPVIGGDGSWIEVDPKDPNVVYAASQFGNAVRVDLKTGER
                     SRFRPRHALGEPALRYNWVTPFLISPHSPNVLWFGTQRLFRSFDRGETWAAVSPDLTS
                     PREAGDVPFGTITTISESQKTFGVLFVGTDEGKVWGTRDGGATWKDLSKGLAKDRWVT
                     RVVASVHDEGTVWVSQSGYRNDDFAPYLWKSTDFGATWASIASGLPAEPVNTVREDPK
                     AKHLLYAGTDLGAYVSLDRGATWNALSGGLPHVPVHDLAIHPKEGDLVLGTHGRSAFV
                     AEAAPLRELTPEVAQKALHAFRVKPATWERARGYGENEFFSWRRVPTTRTLSWWASPA
                     AAGTATVTVKDAKGRVWKEWNAPSVAGFNAVAYDLSADPARAGTNEEALREEAAEAAA
                     KGAAGAKGPEAAEEAAPEEVDGDDAGSKTTPPDIVPLLDDPLRTSRDRYLPPGRYTVE
                     VAVSGARAATRLVVKAPKGEGDRRGGR"
     gene            2483..3181
                     /locus_tag="P5164_05260"
     CDS             2483..3181
                     /locus_tag="P5164_05260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY43338.1"
                     /translation="MTDRHPCVLVVEDDSETRDFVARALGEDGLEVRTASRAETARKL
                     VRDGDVDLVILDLGLPGPSGLDLCAELRREESVVPILMLTARTDVASRVEGLEAGADD
                     YLGKPFALAELRARVRALLRRGSRPGPPGAVLEHAGVRVDVRRRRAFLGAEELPLTRR
                     ELDLLARLVRAGGVVVARETLLEDVWGADTPEAAASLEVIVSRLRRKLRQGGRASLLR
                     NVRGIGYALDEDEA"
     gene            3178..4488
                     /locus_tag="P5164_05265"
     CDS             3178..4488
                     /locus_tag="P5164_05265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRY43339.1"
                     /translation="MIVRSLARRIVAATLLTAAVSSFVFAIASVLALRLLWDRHDAAE
                     LKRTAEAAARIFTLEQAEGEHGAGDAAHEALEQALEPADWGLFRRGRTPLAARGERPA
                     RGEDGRLPAGWIGATRGLEGDVVLEVARRPKEGLPLRRLFWAALLVAAIPSSLVALAV
                     GRRAGRAAARPIEDLVRRLGEIRHPRDVVPAPLEGLPTEAAALERSFASVLGRLGAAL
                     EREASFVRNAAHELRTPLTRIRLRAERAATSASGACRLELQAVTGEVDRLTRLVDALL
                     VLARDESASLDLSETVNLADLVRASAEAGERTVEVAAPDEALVRGDEELLRLAVDNLL
                     DNARKYAPADRLPRALLAAEPDRVTLTVETPGVSLPDEEREQVFERFYRGASARETAP
                     GHGLGLSLARHVARVHGGDVVASASGGERTVFELRLPGWRGAEG"
     gene            4556..6277
                     /locus_tag="P5164_05270"
     CDS             4556..6277
                     /locus_tag="P5164_05270"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HRY43340.1"
                     /translation="MPSARARVLPETLLAILVALLVLVEPFRVPLVDPDEGRYAEIPR
                     EMLASGDWVLPHQNGLVYIEKPPLAYWLVAGSYGLFGVNEAAARLPGKLATVGTMLAL
                     FLFARRRAGERAGALAALFFGGSLLGFGLARIVLIDPILTAAQTAAVLSFAALGEEGS
                     SPRRERALALAFAVAAAAAVLLKGLVGVLLPGGAVLLWTAATGRTRPLRALLSPWAVL
                     TFLALAVPWHVAAASREPGFLSFYFVREHFERFLLPNHRRLGAPWYFLAVVAGGFLPF
                     TPLLPRLRSVWPGRRRAAWAARPLETFLFLWVLLVVGFFSLSKSKLIPYVHPVFPSLA
                     LLLALAVARDEEGSVLRDGERWSIAGLLGVLAAAAAWAGASDDLAVPGLAGPVLGLAV
                     ALLAGFLLVGIGPTLAPERGLLLVGAAWVLFLGGAHLVLPAWARWDSSWPVAAAMKET
                     LRPGDLLVQRGDYVESLPFYAGRLTPLSRIGAGSELTFGRSRDHNGIFQTDEEFSRLW
                     NGPRRVLAVLHREVLGEWAAPGNRRRPYRILATAHRDRWVLVSNDSDGPLARSALGGG
                     PEAAAIR"
     gene            complement(6287..7351)
                     /gene="rsgA"
                     /locus_tag="P5164_05275"
     CDS             complement(6287..7351)
                     /gene="rsgA"
                     /locus_tag="P5164_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRY43341.1"
                     /translation="MPLTRFGWDRDFEAQFENHAREGFAPGRVLTASRETYRLATAAG
                     EIPAELSGRLRFAAESSSDLPAVGDWVAARIYPGDGLAVVDSVLPRRSALVRRSSGRR
                     DEAQVLAANVDLLLVAAPLGGELRERRIERFLALAREAKVRPAVLLTKADLAVDPAAD
                     LAAARAVAGEAPVAVVSARTGAGLDALEPLLPPHRTGALLGPSGAGKSTLVNALLGER
                     RQTTREVRDGDLRGRHATTSRELFTLASGALLVDTPGLRELALWDGAEAIAETYDEIA
                     ARAPGCRFRDCRHEGEPGCAVRAAADEGVIDPDRLAAFEKLRREDESLAQRRALGPAR
                     AERERWRRMMGGSKGPVKLR"
     gene            7642..8733
                     /locus_tag="P5164_05280"
     CDS             7642..8733
                     /locus_tag="P5164_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HRY43342.1"
                     /translation="MYAGFILLVLALLALDLGVFHRKAHVVTVKEALGWSAFWIALGL
                     AFSGFIYLGYENHWLGLGLTPDMMTLRPVAVEGLGTVYNDGASALVKYVTGYLVEKSL
                     AVDNIFVISMIFTFFAVPAIYQHRVLFWGILGALILRGMMIAAGARMIQEFSWIIYVF
                     GGFLILTGIKMLLLKEGETDPNKNLVVRATRKLFPVTERFHGEHFFVRAGTDASHEAE
                     TPGAAVVADRVVDAAKAGTLLATPLLLALVMVEFTDLIFAVDSIPAIFAITADPFLVF
                     TSNVFAILGLRSLYFALAGMIDSFRYLKVALAVVLMVVGVKMMTHTWLKAQLGENFNL
                     YLLAVVLSILAAGVVASLWKSRSEARAAQ"
     gene            complement(8743..9288)
                     /locus_tag="P5164_05285"
     CDS             complement(8743..9288)
                     /locus_tag="P5164_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43343.1"
                     /translation="MSLLAVVAKHLSLRRTPFATVGGVALAIRGVARSTFDVDLLTLD
                     ASVLADAYWSVVTRDGAQIEIRRGDGDDPLRGLVRLLSRDERPVDVFVGRGAWLDGVV
                     ARAEPFDLLDTRVPVVRAADLVLLKLHAGGSQDLWDVTQLLATGDGTLSDEVASRLSD
                     VPRGAAILWRRLHAPHDRCQA"
     gene            complement(9285..9500)
                     /locus_tag="P5164_05290"
     CDS             complement(9285..9500)
                     /locus_tag="P5164_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43344.1"
                     /translation="MTSIGDAFRRKTAEADALLTPAERVARAFSLGDDDAEAFRRPRG
                     LPRAAAEAELAARRRTGRIPSRVAGTG"
     gene            complement(9497..10639)
                     /gene="chrA"
                     /locus_tag="P5164_05295"
     CDS             complement(9497..10639)
                     /gene="chrA"
                     /locus_tag="P5164_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HRY43345.1"
                     /translation="MKETGLGEIAAVFLRLGTTAFGGPAAHVAMMRDEVVRRRGWLTD
                     AELLDLLSAANLIPGPNSTELAIHVGWARRRWPGLAVAGLSFILPAALITAALAWAYV
                     RFGALPETGRLLWGVKPVILAVVVQAIVGLAPHAARTVPLRLLGIAAAAAAALGAHEL
                     VVLLGAGLLVAAARAVRSRRAGPGADLPALGPLLPVAAGAAGLSAIGAVTLPAIFWLF
                     CRTGAVLFGSGYVLVAFLRTDLVERLGWLTEAQLLDAVAAGQVTPGPVFTTATFVGWL
                     LAGPAGAAAATAGIFLPAFVFVALSAPFVPRLRASQTAGAFLDGVNVASVALMAVVTA
                     RLAPAALVDLRAALLGLLAAILLVRYRTGSVVLVLGGAVAGLIIGP"
     gene            10732..12018
                     /locus_tag="P5164_05300"
     CDS             10732..12018
                     /locus_tag="P5164_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRY43346.1"
                     /translation="MTGLFVILALVVANGLFAGAEIAVLTLRRTRLAELVEEERAGSR
                     ALAALRADPEGFLATVQIGITVVGAAAAAFGGATLSSPLAAALEAAGAPKKVADDVAL
                     AVVVVVVSFLSLVVGELVPKSLAMRFAERYALAIARPLALLARLARPAVRFLTACSNA
                     VLRLFGDRTSFVEARLSRDELRTLVEETATTGGLDPRAGEIAARALDLPGVKVGAVMT
                     PRPSVVAIDATATREEIVTIVSRSRFARFPVHDGGLDGATGYVLGREVLARAVEGAAS
                     PLQGIVRDAPFFPETVEALGVLRELQERHVPIGFVVDEQGGFTGLVTLEDLVEELVGE
                     IFHEKEADAVPYREDGPGAWVVEGRAGVRDVNRLLDLELPEDPAWATIAGLILHRLER
                     IPPAGTAFVDEESGLTVEVVESTPRRIARVRLSRPA"
     gene            complement(12024..12545)
                     /locus_tag="P5164_05305"
     CDS             complement(12024..12545)
                     /locus_tag="P5164_05305"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRY43347.1"
                     /translation="MAERRCRLFSVAVLLVGAALLAPDVRAGNPAAPKIGDPAPALAL
                     VDLDGKKLDVSSLRGKVVVVDFWATWCAPCRSQAPKFEALLQKHRGGGLVVVGVSLDE
                     SAEPVLKFRKELGLTYRIALGDEKVAERWGGILGLPVAFVVDRRGRLRARHEGEHDLA
                     ALEADVVRALGEK"
     gene            complement(12538..13074)
                     /locus_tag="P5164_05310"
     CDS             complement(12538..13074)
                     /locus_tag="P5164_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRY43348.1"
                     /translation="MTANPQRPHTRPTPAVPALRRALLLLCALLALAAAPPSLAAAPE
                     KAPVAWDDAKRISIDELARLLKDSAAAKPEMFHVGFRVLFAQAHIPGSRFAGPGASPA
                     GLETLRKAVAEVPKAKTIVLYCGCCPWEKCPNMEQPWRLLVAAGFTDVRVLYIPTNFG
                     RDWVGKGHPSASGSGDRG"
BASE COUNT         1720 a         4690 c         5014 g         1780 t
ORIGIN      
        1 ccgacggcgg cgagcggggc ctctaccgga cgacggacgg cgggaagagc tggaccaggg
       61 tcctcggcgg gcccggccgc accggcgcga tcgacgtggt gcaggacccg aaggacccga
      121 agctcctcta cgccgcgctc tgggagcgcg accgcaagcc gtggaacttc ctcgagtcgg
      181 ggcccgggag cggcgtctac cgctcgcgcg acggcggggc gacctggagc cgcctcgccg
      241 gcggcctccc gcagggcgac gtcgtcggga ggatcggcct cgccgtcacc ccggcgaagc
      301 cgggcgcggt ctgggcggtc gtcgacaacc aggcgcggcg ccccggcgag agggacgagg
      361 agacgccgcc gggcgagctg acgccgcgcc ggctgaaggc gctcaccgcc gagcagttcg
      421 cgaaggtcga gacggccgtc gtcgagcggt tcctccgggc gaacgggttc ccgaaggagc
      481 tgacggcgag agcccttcag cgggacgtcg cctcggggaa ggtgaagctc gcggacgtcg
      541 tggcctacgt cgacgacgcg gaccggcaga tggaggaggt cgagacggtc ggcgtggagg
      601 tctggcgctc cgacgacgcg ggcgagagct ggcggaaggc gcacggcgtc tcgctcgaga
      661 aggtcgccta cacctacggc tactacttcg ggaagatctg ggtcgccccg gacgacccgg
      721 agaggctcta cgtggcgggc gtcccgctgg cgggctcctc cgacggcggg aagagctgga
      781 gcggcctcga ccggcacggc gtccacgtgg accaccacgc cctctggttc gacccgcgcg
      841 acccgcggca tgtcgtcctc gggaacgacg gcggactgaa cctctcgtgg gaccgcgggg
      901 cgacctggac gaaggtgagc aacctccccg tcggccaggt gacgacggtc gcggtcgact
      961 cggccgagcc gtacaacgtc ctcgtcggga tgcaggacaa cggggtctgg cgcgggccgt
     1021 cgacgtacgt ctccggcgcg acgcctcccg acgcctggaa gccggtgatc ggcggcgacg
     1081 gctcctggat cgaggtcgac ccgaaggacc cgaacgtcgt ctacgcggcc tcgcagttcg
     1141 ggaacgcggt ccgcgtggac ctgaagaccg gagagcggag ccgatttcgc ccgcgccacg
     1201 cgctcggcga gccggcgctc cgctacaact gggtgacgcc gttcctcatc tcccctcact
     1261 ctccgaacgt cctctggttc gggacgcaga ggctcttccg ctcgttcgac cgcggcgaga
     1321 cgtgggccgc cgtgagcccg gacctcacct ccccgcgcga ggcgggagac gtcccgttcg
     1381 ggacgatcac gacgatctcc gagtcacaga agacgttcgg cgtcctcttc gtcgggaccg
     1441 acgaggggaa ggtctggggg acgcgtgacg gcggggcgac ctggaaggac ctctcgaagg
     1501 ggctggcgaa ggaccgctgg gtgacgcgcg tcgtcgcctc ggtccacgac gaggggacgg
     1561 tctgggtctc ccagagcggc taccggaacg acgacttcgc gccgtacctc tggaaatcga
     1621 ccgacttcgg cgcgacctgg gcctcgatcg cctccggcct gccggccgag cccgtgaaca
     1681 ccgtccggga ggacccgaag gcgaagcacc tcctctacgc ggggaccgac ctcggggcgt
     1741 acgtctccct cgatcgcggc gcgacctgga acgcgctctc cggagggctg ccgcacgtgc
     1801 cggtccacga cctcgcgatt cacccgaagg agggggacct cgtcctcggg acgcacgggc
     1861 ggagcgcctt cgtcgcggag gcggcgccgc tccgcgagct gacgccggag gtggcgcaga
     1921 aggcgctcca cgccttccgg gtgaagccgg cgacgtggga gcgggcccgc gggtacggcg
     1981 agaacgagtt cttctcctgg cgccgcgtcc cgacgacgcg gaccctctcc tggtgggctt
     2041 cgcccgccgc cgccgggacg gcgacggtca cggtgaagga cgcgaagggg cgcgtctgga
     2101 aggagtggaa cgccccgtcg gtcgccggct tcaacgccgt cgcgtacgac ctctccgccg
     2161 acccggcccg cgccggcacg aacgaggagg cgcttcggga agaggcggcc gaggccgccg
     2221 cgaagggggc ggctggtgcg aagggcccgg aggccgccga agaggctgcg ccggaggagg
     2281 tggacggcga cgacgcgggc tcgaagacga cgccgccgga catcgtccct ctcctcgacg
     2341 acccgctccg gacgagccgc gatcgctacc tcccgccggg tcggtacacc gtcgaggtgg
     2401 ccgtgtcggg ggcgcgggcc gcgacccgtc tcgtcgtgaa ggcgccgaaa ggcgaggggg
     2461 accgaagggg aggacgctga aggtgaccga tcggcatccg tgcgtcctgg tcgtcgagga
     2521 cgactcggag acgcgcgact tcgtggcgcg cgcgctcggc gaggacgggc tcgaggtgcg
     2581 gaccgcgtcc cgcgccgaga cggcccggaa gctcgtccgg gacggcgacg tcgacctcgt
     2641 gatcctcgac ctcgggctcc cgggcccttc ggggctcgac ctctgcgcgg agcttcggcg
     2701 cgaggagagc gtggtcccca tcctgatgct gaccgcgcgc accgacgtcg cctcccgcgt
     2761 cgagggtctc gaggcgggag ccgacgacta cctcggcaag ccgttcgcgc tcgcagagct
     2821 ccgggctcgc gtccgggccc tcctccggcg cgggagtcgc cccggtccgc ccggcgccgt
     2881 cctcgagcac gccggcgtgc gcgtcgacgt ccggcgtcgg cgcgcgttcc tgggcgcgga
     2941 ggagctcccg ctgacgcggc gcgagctgga cctgctcgct cgcctcgtcc gggccggggg
     3001 cgtcgtcgtc gcccgcgaaa cgctgctcga ggacgtctgg ggagccgaca ccccggaggc
     3061 cgccgcgagc ctcgaagtca tcgtcagccg tcttcgccgg aagctccggc agggagggcg
     3121 agcgagcctg ctgcggaacg tacgggggat cggctacgcc ctcgacgagg acgaggcgtg
     3181 atcgtgcgtt cgctcgctcg tcgcatcgtg gcggcgacgc tcctcacggc ggcggtctcc
     3241 tccttcgtct tcgcgattgc atcggtcctc gccctgcggc tcctctggga ccgccacgac
     3301 gccgccgagc tgaagcgcac ggccgaagcc gcggctcgga tcttcacgct cgagcaggcg
     3361 gagggcgagc acggcgcggg ggacgcggcg cacgaggcgc tcgagcaggc gctggagccg
     3421 gcggactggg gccttttccg tcgggggcgc acgcctctcg cggcgcgcgg agaacggccg
     3481 gcgcgaggcg aggacggccg gctccccgcg ggctggatcg gcgcgacgcg agggctcgaa
     3541 ggcgacgtcg tcctcgaggt cgcgcgccgg ccgaaggagg ggctcccgct ccggcggctc
     3601 ttctgggctg cgctgctcgt cgccgcgatc ccttcctcgc tcgtcgcgct cgccgtcggc
     3661 cgccgtgcgg gacgggccgc ggcgcgcccg atcgaggacc tggtgcggcg cctcggggag
     3721 atccggcatc cgcgggacgt cgtcccggcg ccgctcgagg ggctgccgac ggaggccgcc
     3781 gccctggaga ggagcttcgc gagcgtcctc gggcggctcg gcgcggcgct ggagcgggag
     3841 gcgtcgttcg ttcggaacgc ggcccacgag ctccggacgc cgctgacgcg gatccggctc
     3901 cgagccgagc gggccgccac gagcgcgtcg ggcgcctgca ggctcgagct gcaggccgtc
     3961 accggcgagg tggaccggct cacgcggctc gtcgacgcgc tcctcgtcct cgcgcgcgac
     4021 gagtccgcct ccctcgacct gtccgagacg gtcaacctcg cggacctggt gagggcctcg
     4081 gcggaggcgg gggagcggac ggtggaggtc gccgcacccg acgaggcgct cgttcgcggc
     4141 gacgaggagc tcctccgtct cgccgtcgac aacctgctcg acaacgcccg gaagtacgcg
     4201 ccggccgacc ggcttccccg ggcgctcctg gcggcggagc cggaccgtgt cacgctgacc
     4261 gtcgagactc ccggcgtctc ccttccggac gaggagcgcg agcaggtctt cgaacgcttc
     4321 taccgcgggg cctccgcgcg cgagacggcg ccgggccacg ggctcgggct ctccctggcg
     4381 cggcacgtgg cgcgggtcca cggtggagac gtcgtggcca gcgcgtcggg cggtgagcgg
     4441 accgtcttcg agctgcggct cccgggctgg aggggcgcgg agggctgatc cggacgttcg
     4501 gcagccgttc gggaagattc ggacgccatt cggcccgccg ggcgaccgtg accggatgcc
     4561 gagcgcccgc gcccgtgtcc tgccggaaac gctcctcgcg atcctcgtcg cgctcctcgt
     4621 cctcgtcgag ccgttccggg tcccgctggt cgacccggac gaggggcgct acgccgagat
     4681 cccgcgggag atgctcgctt cgggggactg ggtcctcccg catcagaacg gcctcgtcta
     4741 catcgagaag ccgcccctcg cctactggct cgtcgccggc tcgtacggcc tcttcggcgt
     4801 gaacgaggcg gcggcacgcc tcccggggaa gctcgccacg gtcggcacca tgctcgcgct
     4861 cttcctcttc gcccgccgcc gggcagggga gcgggcggga gcgctggccg cgctcttctt
     4921 cggcggctcc cttctcggct tcggcctcgc aagaatcgtc ctcatcgacc cgatcctgac
     4981 ggccgcgcag accgcggccg tcctctcgtt cgccgcgctc ggcgaggagg gctcgagccc
     5041 gcggcgcgag agggcgctcg cgctcgcgtt cgccgtggcg gccgcggcag ccgtcctcct
     5101 gaagggcctc gtcggcgtcc tccttcccgg gggcgcggtg ctcctctgga ccgcggcgac
     5161 ggggaggacg cggccgctcc gcgccctcct ctcgccgtgg gccgtcctca cgttcctcgc
     5221 cctcgcggtc ccgtggcacg tcgccgcggc gagccgcgag cccgggttcc tcagcttcta
     5281 tttcgtccgc gagcacttcg agcgttttct tctcccgaac caccgccgcc tcggcgcccc
     5341 gtggtatttc ctcgcggtcg tcgcgggagg cttcctcccc ttcacgccgc tcctgccgcg
     5401 tctccgatcg gtctggccgg ggcgccgccg cgccgcctgg gccgcgcgtc ccctcgagac
     5461 gttcctgttc ctctgggtcc tcctcgtcgt cggcttcttc tccctttcga agtcgaagct
     5521 gatcccctac gtccaccccg tcttccccag cctcgcgctc ctgctggcgc tcgccgtggc
     5581 gcgcgacgag gaggggtccg tcctcaggga cggcgagcgc tggagcatcg ccgggctgct
     5641 cggcgtcctg gcggcggccg cggcgtgggc cggcgcctcg gacgacctcg ccgtcccggg
     5701 ccttgccgga ccggtcctcg gcctcgccgt ggcgctcctc gccggcttcc tcctcgtcgg
     5761 gatcggcccg acgctggcgc cggagcgggg actcctgctc gtcggcgcgg cctgggtcct
     5821 cttcctcggc ggagcgcacc tcgtccttcc ggcctgggcc cggtgggaca gcagctggcc
     5881 ggtcgccgcg gcgatgaagg agacgctgcg gccgggcgac ctcctcgtcc agcgcgggga
     5941 ctacgtcgag tcccttccgt tctacgccgg ccgtctgacc ccgctgagcc gtatcggcgc
     6001 cgggagcgag ctcaccttcg gcaggagccg cgaccacaac ggcatctttc agaccgacga
     6061 ggagttctcg cggctctgga acggcccgcg gcgggtcctc gccgttctcc accgggaggt
     6121 cctcggcgag tgggcggcgc ccgggaaccg gagacggccc tatcggatcc tcgcgacggc
     6181 gcaccgggac cgctgggtcc tcgtctcgaa cgactcggac ggacctctcg cccgttccgc
     6241 gctcgggggc ggaccggagg cggcggcgat ccgctgacgg cgctcctcac cgcagcttca
     6301 ccggtccctt cgacccgccc atcattcgcc gccagcgctc ccgctcggcg cgggccggcc
     6361 cgagggcgcg ccgctgggcg agcgactcgt cctcgcggcg gagcttctcg aacgcggcga
     6421 ggcgatccgg gtcgatgacg ccctcgtccg cggccgcccg gacggcgcag ccgggctctc
     6481 cctcgtggcg gcagtcgcgg aaccggcagc ccggggctcg cgccgcgatc tcgtcgtagg
     6541 tctcggcgat cgcctccgct ccatcccaga gcgcgagctc gcggaggccc ggcgtgtcga
     6601 cgaggagggc gccgctcgcg agggtgaaga gctcccggct cgtcgtcgcg tgccggccgc
     6661 gcaggtcgcc gtcgcggacc tctcgggtcg tctggcggcg ctcgccgagg agggcgttga
     6721 cgagcgtcga cttgccggcc cccgaagggc ccaggagcgc gccggttcgg tgcgggggca
     6781 ggaggggctc gagcgcgtcg aggccggcgc cggtccgcgc gcttacgacc gcgaccggcg
     6841 cctcgccggc gacggcccgg gccgcggcga ggtcggcagc ggggtcgacg gcgaggtcgg
     6901 ccttcgtcag gaggacggcg gggcggacct tcgcctcgcg cgcgagggcg aggaacctct
     6961 cgatcctccg ctcgcgcagc tctccgccga gcggcgcggc gacgaggagg aggtcgacgt
     7021 tcgccgcgag gacctgcgcc tcgtcccgcc ggccggacga gcgccggacg agggcggacc
     7081 ggcgcggcag gaccgagtcg acgacggcca ggccgtcgcc ggggtagatc cgagcggcca
     7141 cccagtcgcc gacggcgggg aggtcggagg aggactctgc ggcgaatcgg agccggccgg
     7201 agagctcggc cgggatctct ccggcggcgg tggccaggcg ataggtctcg cgggaagcgg
     7261 tcaggacgcg gccgggggcg aacccttcgc gcgcgtggtt ctcgaactgg gcttcgaaat
     7321 cgcggtccca cccgaatcgg gtcaggggca tggcgggctt ctccatctcg gtttgcggac
     7381 ggggaacggg agcggggcgc cgcggcgaaa acaacatcgc cgggcaggtc gcccgtctcg
     7441 atcaggggcg cgctacgggg cgcgccggcc cgtcaggcaa ggaccgattg cagaagccgt
     7501 ctcaggcccg cgatcatagc gcgggtcgtc cattccgagt cggtccgggc agggaatttc
     7561 ggcggtgcct cgcgcgttag cgccccgtcc gggttccggc gccgagcgtc ggccccgaaa
     7621 ggagcttccc gagtgttctg gatgtacgcc ggcttcatcc tcctggtcct ggcccttctc
     7681 gcgctcgacc tcggggtctt ccacaggaag gcccacgtcg tgaccgtgaa ggaggcgctc
     7741 ggctggtccg ccttctggat cgcgctcggg ctcgccttca gcgggttcat ctacctcggg
     7801 tacgagaacc actggctggg gctcgggctg acgccggaca tgatgacgct gcggcccgtc
     7861 gccgtggagg ggctcgggac ggtctacaac gacggcgcgt cggcgctcgt caagtacgtg
     7921 acgggctacc tcgtcgagaa gtcgctcgcc gtcgacaaca tcttcgtcat cagcatgatc
     7981 ttcacgttct tcgcggtgcc ggcgatctac cagcaccggg tcctcttctg ggggatcctc
     8041 ggcgcgctga tcctgcgggg gatgatgatc gcggcggggg cgcggatgat ccaggagttc
     8101 tcctggatca tctacgtctt cggaggcttc ctgatcctga ccgggatcaa gatgctcctc
     8161 ctgaaggagg gcgagaccga cccgaacaag aacctcgtcg ttcgtgcgac tcggaagctc
     8221 ttcccggtca ccgagcgttt ccacggcgag cacttcttcg tgagggccgg gaccgacgcc
     8281 tcgcacgaag cggagacccc gggcgcggcg gtcgtcgccg accgggtcgt cgacgcggcg
     8341 aaggccggca cgctcctcgc gacgcccctc ctcctcgccc tcgtcatggt ggagttcacc
     8401 gacctgatct tcgcggtcga ctcgatcccg gccatcttcg cgatcacggc cgacccgttc
     8461 ctcgtcttca cgagcaacgt cttcgcgatc ctcggcctgc gaagcctcta cttcgccctg
     8521 gcggggatga tcgactcgtt ccgctacctc aaggtggcgc tcgccgtcgt cctgatggtc
     8581 gtcggggtca agatgatgac ccacacctgg ctcaaggcgc agctcggcga gaacttcaac
     8641 ctctacctcc tcgccgtcgt cctctcgatc ctcgccgcgg gggtcgtcgc gtctctctgg
     8701 aagagccgga gcgaagcgcg agcggcccag taatacacga tttcacgcct ggcaccgatc
     8761 gtggggggcg tggaggcggc gccagaggat ggcggctccg cgggggacgt cggagaggcg
     8821 ggaggcgacc tcgtcggaga gggtgccgtc gcctgtggcg aggagctggg tgacgtccca
     8881 gaggtcctgg gagccgccgg cgtggagctt gaggaggacg aggtcggctg cgcggacgac
     8941 ggggacgcgg gtgtcgagga ggtcgaacgg ctccgcgcgc gccacgaccc cgtcgagcca
     9001 ggcgccgcgg ccgacgaaga cgtcgacggg gcgctcgtcg cgcgagagga ggcggacgag
     9061 gccgcgcaga gggtcgtcgc cgtcgcctcg ccggatctcg atctgcgcgc cgtcgcgcgt
     9121 caccacggac cagtaggcgt cggcgaggac ggacgcgtcg agggtcagga ggtcgacgtc
     9181 gaacgtcgaa cgggcgacgc cccggatcgc gagggcgacg cctccgaccg tcgcgaaagg
     9241 ggtgcggcgg agggagaggt gcttcgcgac gacggcgaga aggctcatcc ggttcccgcc
     9301 acgcggctcg ggatccgtcc ggtccgacgc ctcgcggcga gctcggcctc ggccgccgcg
     9361 cgcggcagtc cgcgggggcg gcggaaggct tcggcgtcgt cgtccccgag agagaacgcc
     9421 ctcgcgaccc gctcggcggg cgtgaggaga gcgtcggctt cggcggtctt ccgccggaat
     9481 gcgtcgccga tggacgtcac ggcccgatta tgagtcccgc gacggctccc ccgaggacca
     9541 gaacgaccga gcctgtgcgg tacctcacga ggaggatggc ggcgaggaga ccgaggagcg
     9601 cggcgcgcag gtcgacgagg gcggccggag cgagccgggc cgtcacgacg gccatcagcg
     9661 cgacggaggc gacgttcacg ccgtcgagga acgcgccggc cgtctgcgag gcgcgcagcc
     9721 gcgggacgaa gggggccgac agcgcgacga agacgaacgc ggggaggaag atcccggcgg
     9781 tggcggcggc ggcgcccgcg gggccggcga ggagccaccc gacgaacgtg gcggtcgtga
     9841 agacggggcc cggcgtcacc tgcccggcgg cgaccgcgtc gaggagctgc gcctccgtga
     9901 gccagccgag ccgttcgacg aggtcggtcc ggaggaacgc gacgaggacg tagccgctgc
     9961 cgaagaggac ggccccggtc cggcagaaga gccagaagat cgccgggagc gtgacggcgc
    10021 cgatcgcgga gaggccggcg gctccggccg cgaccgggag gagcggcccg agcgcgggga
    10081 ggtcagcgcc cggaccggcg cggcgggagc gtacggcccg cgcggccgcg acgaggagcc
    10141 cggcgccgag gaggacgacg agctcgtgcg cgccgagcgc ggccgcggcc gcggcggcga
    10201 tgccgaggag ccggagcggg acggtgcggg cagcatgcgg agccaggccg acgatcgcct
    10261 ggacgacgac cgcgaggatg accggcttga cgccccagag gagccgtccc gtctccggga
    10321 gcgccccgaa gcggacgtag gcccacgcga gcgcggcggt gatcagcgcg gcggggagga
    10381 tgaacgagag gccggcgacg gcgaggccgg gccaacggcg ccgggcccag ccgacgtgaa
    10441 tcgcgagctc ggtcgagttc ggcccgggga tcaggttcgc ggccgagagg aggtcgagga
    10501 gctccgcgtc ggtcagccac ccgcgccgcc tcaccacctc gtcgcgcatc atcgcgacgt
    10561 gcgccgccgg ccccccaaag gcggtcgttc ccagccgcag gaagaccgcg gcgatctctc
    10621 ccagccccgt ctccttcatc cccgcgcaga cactatcccc aaacggtgcc tccccaaacg
    10681 gtgccaggcg tgaaatcgtg tattgtttgc ggctccccgt agaatcaccg ggtgaccggg
    10741 ctcttcgtca tcctcgcgct cgtcgtcgcg aacggcctct tcgcgggggc ggagatcgcg
    10801 gtcctgacgc tccggcggac ccgtctcgcc gagctcgtcg aggaggaacg ggcgggttcg
    10861 cgggccctcg cggcgctccg ggcggacccc gaggggttcc tcgcgacggt ccagatcggg
    10921 atcacggtcg tcggtgcggc ggccgcggcc ttcggcgggg cgacgctctc ctccccgctc
    10981 gccgcggcgc tcgaagcggc cggggcgccg aagaaggtgg cggacgacgt cgctctcgcg
    11041 gtcgtcgtcg tcgtcgtctc cttcctgtcg ctcgtggtcg gggagctcgt tccgaagtcg
    11101 ctcgcgatgc gcttcgcgga gaggtacgcg ctcgccatcg cccggcccct cgctctcctc
    11161 gcccgcctcg cccgtccggc ggtccgcttc ctgaccgcct gctcgaacgc ggtgctccgg
    11221 ctcttcgggg accggacgtc gttcgtcgag gcccggctct cgagggacga gctccggacg
    11281 ctcgtcgagg agacggcgac gacgggaggg ctcgacccgc gggcggggga gatcgcggcg
    11341 cgggcgctcg acctgccggg ggtgaaggtc ggcgcggtca tgacgccccg gccctcggtc
    11401 gtcgcgatcg acgcgacggc cacgcgcgag gagatcgtca cgatcgtcag ccggagccgg
    11461 ttcgcgcgat tcccggtcca cgacggaggc ctcgacggcg cgaccggcta cgtcctcggc
    11521 cgggaggtgc tcgcgcgcgc cgtcgaaggc gcggcttcgc cgcttcaggg gatcgtccgc
    11581 gacgcgccgt tcttcccgga gacggtggag gcgctcggcg tccttcgcga gctccaggag
    11641 cggcacgttc cgatcgggtt cgtcgtcgac gagcagggcg ggttcaccgg cctcgtgacg
    11701 ctcgaggacc tcgtcgagga gctcgtcgga gagatcttcc acgagaagga ggccgacgcc
    11761 gtgccgtacc gcgaggacgg cccgggggcg tgggtcgtcg aggggcgcgc cggcgtccgc
    11821 gacgtgaacc ggctgctcga cctcgagctg cccgaggacc cggcctgggc gacgatcgcg
    11881 gggctcatcc tccatcgcct cgagcggata ccgccggcgg ggacggcgtt cgtcgacgag
    11941 gagtcgggcc tgacggtcga ggtcgtcgag agcacgcccc gacggatcgc gcgcgttcgc
    12001 ctgtcgcggc ccgcctgacg ccgctacttc tctccgagcg cccggaccac gtccgcctcg
    12061 agggcggcga ggtcgtgctc gccctcgtgc cgcgcgcgaa ggcgccccct ccggtcgacg
    12121 acgaacgcga ccggaaggcc gaggatgccg ccccagcgct cggccacctt ctcgtcgccg
    12181 agggcgatcc ggtacgtcag gccgagctcc ttccggaact tcaggaccgg ctcggccgac
    12241 tcgtcgagcg agacgccgac gacgacgagc ccgccgccgc ggtgcttctg cagcagcgct
    12301 tcgaacttcg gggcctggga gcggcagggc gcgcaccagg tggcccagaa atcgacgacg
    12361 acgaccttgc cccggagcga agagacatcg agcttcttcc cgtcgaggtc gacgagggcg
    12421 agggcggggg ccgggtcgcc gatcttcggc gcggcagggt tcccggcccg gacgtcgggc
    12481 gcgagcagcg cggcaccgac gaggaggacc gcgacggaga agaggcgaca gcgccgctca
    12541 gccacgatcg ccgcttcccg aggccgaggg atggcccttc ccgacccagt cccggccgaa
    12601 gttcgtcggg atgtagagga cgcggacgtc ggtgaacccg gccgcgacga ggagccgcca
    12661 cggctgctcc atgttcgggc acttctccca cgggcagcag ccgcagtaga ggacgatcgt
    12721 cttcgccttc ggcacctcgg ccacggcctt ccggagcgtc tcgaggccgg cggggctcgc
    12781 cccgggaccg gcgaagcggg agccggggat gtgagcctgg gcgaagagga cccgaaatcc
    12841 gacgtggaac atctccggct ttgcggccgc ggagtccttc aggaggcggg cgagctcgtc
    12901 gatcgagatc cgcttcgcgt cgtcccaggc gaccggcgcc ttctccggcg cagcggccag
    12961 cgagggaggc gcggcggcca gagcgaggag cgcgcagaga agaaggaggg cgcgtcggag
    13021 ggcggggacg gccggggtcg ggcgcgtgtg cggccgctgc gggtttgcgg tcatggcgcg
    13081 cgatcctaca gaggcgttca cgaaagcggg agcccgccgc ggcgagggga tcggccgccc
    13141 tcgagcccgg gctgagagcg tgtgaagtaa tcgcgccgct ggggttgcaa acagagaacg
    13201 gcgt
//
LOCUS       DAOTEZ010000067        13107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3303_length_13107_cov_6.439626, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000067 DAOTEZ010000000
VERSION     DAOTEZ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13107
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3303_length_13107_cov_6.439626"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="P5164_05315"
     CDS             <1..958
                     /locus_tag="P5164_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRY43349.1"
                     /translation="FIREAEVFPSRQIEFARLNLTHTVLSKRWLLRLVKEGRVEGWDD
                     PRMPTIAGLRRRGYTPEAIRDFAERIGLAKRDSVVDVALLEHCLREDLNRRAPRGMAV
                     IDPVKVVLTNWPEGVVEELEAVNNPEDPSAGTRKVAFGRELFVERDDFREVPPPKYHR
                     LSPGNEVRLRWGFIIRCEEVVKDADGNVVELRCTYDPTTKSGEAGAARKVKGTIHWVS
                     AAHAVPAEVRLYDRLFTREDPNDAPEGQDWLSNLNPASLEVRQAFVEPALAMGVPGAY
                     FQFERLGYFTVDADSVPGKPVFNRSVGLRDTWAKIERKGRAA"
     gene            1072..1416
                     /locus_tag="P5164_05320"
     CDS             1072..1416
                     /locus_tag="P5164_05320"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRY43350.1"
                     /translation="MMGVQLWLSSRSRLPDALSPFPGSDKLAHGGWFFLLALLAWRAA
                     RDGEGWARRRRVVGLLVAAAAWAVSDEAHQSFVPGRAVEAADLLADVAGTALALALAE
                     GVLFARRPPRAG"
     gene            complement(1456..1529)
                     /locus_tag="P5164_05325"
     tRNA            complement(1456..1529)
                     /locus_tag="P5164_05325"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1495..1497),aa:Gln,seq:ctg)
     gene            complement(1587..2126)
                     /locus_tag="P5164_05330"
     CDS             complement(1587..2126)
                     /locus_tag="P5164_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43351.1"
                     /translation="MPVDADRLHQVVESLLKGDKSLNEKDVKAAAGKQLKVRPEEIGA
                     GAIREVRRKLGIDRPAALAWARAHLAKAPTTEARVVIEAVAEKFGIRLGPPDVTRLRP
                     QEARRPRKNGRKKAGRAKVLKPEVVDAAPKKGRGKAAAAEPVVVAGPVKGEVSVTFQG
                     RGTPEDLARFFLSLSKKPR"
     gene            complement(2194..2505)
                     /locus_tag="P5164_05335"
     CDS             complement(2194..2505)
                     /locus_tag="P5164_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRY43352.1"
                     /translation="MTVEELKALLDSGEPPFVLDVREPLELQTAPFPFPVHHVPMREV
                     PQHLGEVPREGTVVVACRSGQRSATVVQFLRKNGVPGAVNLEGGILAWSSRIDPSVPR
                     Y"
     gene            complement(2552..2905)
                     /locus_tag="P5164_05340"
     CDS             complement(2552..2905)
                     /locus_tag="P5164_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020150591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HRY43353.1"
                     /translation="MAPSPANVLVIGGELAVSWSDGTETYLPLKALRRLCPCAGCAGE
                     RDLFGRLAKPPQKPYTNASFEVAATSPVGGYALQLFWKDGHSDGLYPWKVLKEWGERL
                     PELPPLEVKTLLPAL"
     gene            complement(2944..4323)
                     /locus_tag="P5164_05345"
     CDS             complement(2944..4323)
                     /locus_tag="P5164_05345"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRY43354.1"
                     /translation="MKQKAALPELPLPLRQAFAIVVAATLSTGAFAAPAPETEPVVKR
                     AAPGESPRLSIAHDPLKCLSTEARPLVDARVLPGKELEQGFVYFRASGTEDYYYVLMR
                     VRAPEDLVGELPRPLPGIPAIDYYVQALDLASLPKTTPAYAPPVTDKTVCRDERAATT
                     VVTSASPREGLTVGLTRAGQEPVPPGFNKDDIAKVILVTGAVVSLSSALSSGTGAAAG
                     AAAAKAGGLSAGTVFGIGAGVVVAGVAVAASQSGGETNAAPTIGSASVTPLYGSVPLQ
                     VTATATASDPDNDPLTYSWNFGSGSATGSTASFTFQSAGTFPVSLTVTDGKGGSATNA
                     SVATIRVDAQGTPQYLTGLASWSGDADLDVRIAGPGGIDVAAQQGGRRLPAACGAGNR
                     TESVVYEGNRLPFGTYTLYVKHAATCAGSPTTARFSYSVQATSGSKCAGLLDVAPGAE
                     VQACTFSFP"
     gene            complement(4478..6337)
                     /locus_tag="P5164_05350"
     CDS             complement(4478..6337)
                     /locus_tag="P5164_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRY43355.1"
                     /translation="MRKQLGKYLIEAELGRGGMGVVYRAHQPSLERTVAIKLLSSDLI
                     GDPDGVRRFRLEAKSVAKLNHPNIVQVYDIEEEENLIYLVMEFVDGESLDSLISKEVL
                     TESRCLKIVAEIADALHYAHEKGIVHRDVKPANVLMTAEGRVKVADFGLAYLIDREGG
                     TTRTGFLVGSPNYMSPEQAMGQKVDRRSDVYALGVVLFRMLTGRVPFVAESSHAVLFM
                     QVQQEPPDPRDMNKGISLVTRGLVLKALNKKPEARFQTMAELRDAALRQAAALSAGMA
                     GELSLDEGTVALDTSEARAASERAHVEAHGISMPPVAPEPTKATAQIAAPPAPPVAEP
                     PAPAPPGVARRGPPRPAARPATPARSPILPIAVVVSGLLFVGLGGTLLYRSGLIPGLG
                     RPAATPPLPQPTAVPTPGIPLPTPTAVFPPATPFPEPTVVPTEAPPLPTPRVEPTKPI
                     VVKVPTPRPTARVVVERPAPTPAAGGGLCVQMGPTSFAQGKTSGFAPGFDVGSGPQAR
                     AAKPDLGRFYIEFEVRPSRPQQGQLVTIEAYFSNEAERDVALDHVEENVGMSVTNFRP
                     VVSPPPPAKVRVGRKEPVWAFQGPLGASFLKTIKVTDGSGDSWSRTLEIGPCP"
     gene            6356..7600
                     /locus_tag="P5164_05355"
     CDS             6356..7600
                     /locus_tag="P5164_05355"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY43356.1"
                     /translation="MTECTSAAAPSRPSPPPPGEEAWARAAEAAVEALRLDPQRTRAG
                     LLCVAAGAAVLVALVSIVQGGRRELLRTVEAAGPSNVFVRAERGAPTPLTTAGLLAAR
                     ARFPGLREATGVRVVPAAVRGGTLELPATVYGVGPEALGLFGLRRARGRLLGPHDARS
                     RSRTALLGARLAAGLARHGDPVGTRLTAGGETYEVVGVLEPSASVAGSGGELAGVEWD
                     SGLLVPLGAEPGAANAPEGEMPVDLLALRFSDPGEAARATRLLSPLVPSGAAVTTPTQ
                     AIEQYRAAHRSFDRVVLLVSILTAASAAFGVTNLLRASVRARSLEIGLRRAAGARRED
                     VRLQFLAEGLLLGFGGGLLGILLGVLLSVTLLSRAGWTPHFAPATLLLLAGGSAAFGI
                     AAGIRPANEAAALDPAATLRLE"
     gene            7597..9003
                     /locus_tag="P5164_05360"
     CDS             7597..9003
                     /locus_tag="P5164_05360"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRY43357.1"
                     /translation="MIVPLLLAAVLSAPPASEPLSVERAVELALTGNPELLDAVDRAA
                     LAAATLSAARSRYLPQVTPYLLPSFRDGDGAAVSRYGVSVTEQLPFGPRLSGNVEASR
                     LEGAEGWDSSHSLTLSQPLLRGLDPAVTREPLRAARRQVAAEERNLGVQRRLTVVAVW
                     SAYLSAALQDELVREAAGRTTRAGRILEASRAKEEIGSVSRLDVLRAEQLFAQARSQE
                     NDARNAREDAHDLLARLLGLPAGSRFRLEPPTGLPVEVPGLEEALDAAAASREELVEA
                     RERVTDAEAQLRIARSLLLPSLDAGLTWSASGRADGFGGAFGRPGGSTWTLGFSSQAP
                     LNLGAQLSARTQAEVSLRIAGRAVEVLEADVARLVRSAGRRLATSRERLALDTANEEV
                     ARMQLEVAELRFGKGLTDNFFVVDAEGLYNAARVSLLTSRREVLLDGLRLLAEAGLLR
                     PEAFLPRPAPSPYTSSRR"
     gene            9000..10484
                     /locus_tag="P5164_05365"
     CDS             9000..10484
                     /locus_tag="P5164_05365"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY43358.1"
                     /translation="MIEAESVAPAVRRFREAFSRLAALPPRTKALLLGGTAVALLGAG
                     LAVRQAVVSDAVGEVRAVRGPLRITVAVAGALAPARAESYGPEIPGVELKVVELVPEG
                     TWVAPGALLVRFDDGPFRRELETARSRLVQTSGEAEQARQALRALLATQRSERLEAET
                     AFARAELDLSTFVNGLAPLQVQQSAAAVERAKREAAESTQKLEGLAPFAEKGYVSRDE
                     LRAAERRATEAAADLVLAEQQHRTLTRFTHPQLLAQKSQEVESRRNALRVVSEKTSAQ
                     AAQAKAAADLASARAAEAAAAVADVERKIALCTVEARSSGLAVHREIFEKGGEKRRVR
                     VGDAVFAGQPVLDLPDLSEMLVETRVKEGEIHRVAPGQPAEVRLEAFPDEVFAATVLR
                     LGALTAGDRGEARSFPLVLKLAKADPRLRPGMTAQALVLAGEVADAVQVPIDAVRYEG
                     DAAVCTVKTVTGAETRRVKTGRSNAFFVEITEGLAEGDVVLLAR"
     gene            10560..10880
                     /locus_tag="P5164_05370"
     CDS             10560..10880
                     /locus_tag="P5164_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43359.1"
                     /translation="MDRERWSSYPAATEEERALLAVLNGTAVRRTLPVVSSRGDERSV
                     EATLAVEAAEPFDGKAEVVVRLVFHGPAPADGERLAEDPWDLLDAFLREAEGEARRRA
                     SARR"
     gene            10877..>13107
                     /locus_tag="P5164_05375"
     CDS             10877..>13107
                     /locus_tag="P5164_05375"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRY43360.1"
                     /translation="MTVRRRGSAAGDPLDLFHAAVAAWFRETFPEGPTEAQRLAWPAI
                     ARGESVLLVSPTGTGKTLAAFLVALDAILRRRLAGDDAPRLATLYVSPLKALDNDVHR
                     NLERPREGIAAQLGAGRVPITTAVRTGDTPSRERTSQLKAPPDVLITTPESLYLMLTG
                     GGRRALEGVSTVVVDEVHSVAGTKRGSHLALSLERLEEVIARAGGAPPQRVALSATVA
                     PVDVVASFAAGTGRKMNPLVVPAKKALDLVVASPAPDFRELPDGSAWGPAVEAVVKEV
                     ESRKTTLVFCNNRRLAEKVSRRLSDATGADVPTHHGSVSRPRREEIEKELKAGTLPVL
                     VATGSLELGIDVGHVDSVVLLESPKGIARGLQRVGRSGHLVGETARGLFVPLYLDDLF
                     ESAATAEAMREGAVEETRPPDFPLDVLAQQLVAEAVARAADGLAVTSAELFALARKAY
                     PYRALPRSLFLETLAMLSGKYPRERFKELAPKLVWDRATDRVTPLPGARIAALLDGGT
                     IGDRGTFRAVLPDRKTLVGELDEEFVHETKEGDVFLLGSRSWRAVEIGHSAVVVEEAA
                     GLPAPRMPFWRGEGLGRTGDLGERIGRLRRFVAERLDDPGLNEAVATRYATTPDATAA
                     IVGAVRREATESGGLASDLAVVFETFPNDLGDPCIVVRSLFGRGVNLPWSLVLSAVLR
                     EETGVDVETVASDDGILLRIPGAEREVPLERLSRISPAEAKERLLAQLPNSPMFGARF
                     RENA"
BASE COUNT         1618 a         5005 c         4708 g         1776 t
ORIGIN      
        1 gttcatccgc gaggccgagg tcttcccctc gcggcagatc gagttcgccc gcctcaacct
       61 cacgcacacg gtcctctcca agcgctggct cctgcgcctc gtcaaggaag gccgcgtcga
      121 gggctgggac gatccgcgca tgccgaccat cgcgggcctg cgccgccgcg gctacacgcc
      181 cgaggcgatc cgcgacttcg ccgagaggat cggcctcgcc aagcgcgaca gcgtcgtcga
      241 cgtcgccctc ctcgagcact gcctgaggga ggacctcaac cgccgcgcgc cgcgcgggat
      301 ggccgtgatc gacccggtga aggtcgtcct gacgaactgg cccgagggcg tcgtcgagga
      361 gctcgaggcg gtgaacaacc cagaggaccc gtcggccggg acgcggaagg tcgccttcgg
      421 ccgcgagctc ttcgtcgagc gggacgactt ccgcgaggtg ccgcccccga agtaccaccg
      481 cctctcgccc ggcaacgagg tccgcctccg ctggggcttc atcatcaggt gcgaggaggt
      541 cgtcaaggac gccgacggga acgtcgtcga gctccgctgc acgtacgacc cgacgacgaa
      601 gagcggcgaa gcgggggcgg cgcggaaggt gaaggggacg attcactggg tctcggcggc
      661 gcacgccgtg ccggccgagg tgcgcctcta cgaccgcctc ttcacgaggg aagacccgaa
      721 cgacgccccc gaagggcagg actggctctc gaacctgaac ccggcctcgc tcgaggtgcg
      781 gcaggccttc gtcgagccgg ccctcgctat gggcgtcccc ggcgcgtact tccagttcga
      841 gcggctcggc tacttcaccg tcgacgccga ctcggtgccc ggcaagccgg tcttcaaccg
      901 ttccgtcggg ctccgggaca cgtgggcgaa gatcgagagg aaaggacggg cggcgtagac
      961 cgacccgccg acgagaggac gatcgggcac gggacgagac cttccgcgcc tggcgccgct
     1021 ccgaaggccc cgcggggccg gaggctcctc ctctggctcc cggtggccct gatgatgggg
     1081 gtccagctct ggctctcgtc ccggagccgg ctgccggacg ccctctcccc gtttccgggt
     1141 tcggacaagc tggcccacgg gggctggttc ttcctcctcg cgctcctcgc gtggcgggcc
     1201 gcgcgggacg gagaaggctg ggcgcgccgc cgccgggtcg tcgggctgct cgtggccgcc
     1261 gcggcctggg cggtcagcga cgaggcgcac cagtcgttcg tgccggggcg ggcggtggag
     1321 gcggccgacc tccttgccga cgtcgccggc acggccctcg cgctggccct cgccgaaggg
     1381 gtcctcttcg cacgccggcc cccccgcgcc ggctgaaacg aaaacgccgc ccgtgggcgg
     1441 cgtgtctcgt caggttggct ggggggcagg gattcgaacc ccgataggca gatccagagt
     1501 ctgcagtcct accattggac gaccccccaa cctggagggg cgggcattat gagggccggt
     1561 cgggcggcgg tcaaccgccg accgcctcag cggggcttct tcgagaggga aaggaagaac
     1621 cgggcgaggt cctccggcgt cccgcgcccc tggaacgtca cggagacctc tccctttacg
     1681 ggtccggcga cgacgaccgg ttccgcggca gccgccttcc cgcgcccctt cttcggggcg
     1741 gcatcgacga cctcgggctt caggaccttc gccctccccg ccttcttccg gccgttcttc
     1801 cgggggcgac gggcctcctg cgggcgaagc cgggtcacgt ccggcggacc caggcgaatg
     1861 ccgaacttct ccgcgaccgc ctcgatgacg acccgcgcct ccgtcgtcgg tgcctttgcg
     1921 agatgcgccc gcgcccaggc gagcgctgcc gggcggtcga ttccgagctt cctccggacc
     1981 tcacggatcg cccccgcgcc gatctcctcc ggccggacct tcagctgctt tccggcggcg
     2041 gctttgacgt ccttctcgtt caacgatttg tcgcctttca ggagcgactc gacgacctga
     2101 tggagccggt cggcgtctac gggcatggat cctccttctc ggggctcgcc ttgtgcgagt
     2161 tcggcaatta tcacccaaaa ggggagcccc ggctcagtac cggggcacgc tcgggtcgat
     2221 ccggctcgac caggcgagga tccctccctc gaggttcacg gcgcccggaa cgccgttctt
     2281 ccggaggaac tggacgaccg tggcgctccg ctggccgctc cggcaggcga cgacgacggt
     2341 gccttcgcgg gggacctcgc cgaggtgctg cgggacctcg cgcatcggga cgtgatggac
     2401 ggggaagggg aacggcgccg tctggagctc gagcggctcg cggacatcca ggacgaaggg
     2461 tggctccccg gaatcgagaa gcgccttgag ctcttcgacc gtcatcgcgt tcccatccct
     2521 ctccggaccg tccgtcgcgt cgggcgagcc gtcacaacgc tggaaggagc gtcttcacct
     2581 cgaggggcgg aagctcgggc agcctctcgc cccattcctt caggaccttc cacggataga
     2641 gcccgtcgct gtgcccgtcc ttccagaaga gctgcagcgc gtagccgccg acggggctcg
     2701 tcgcggcgac ctcgaacgag gcgttcgtgt acggcttctg ggggggcttc gcgaggcgcc
     2761 cgaagaggtc gcgctcgccg gcgcagccgg cacacgggca gaggcggcga agcgccttca
     2821 gggggaggta ggtctcggtg ccgtccgacc aggagacggc gagctcgccg ccgatgacga
     2881 ggacgttcgc gggagacggg gccatgtccg gatcgtaccc cgcctcccgc gcctcccggc
     2941 tcctcagggg aagctgaacg tgcaggcctg gacctccgcg ccgggagcga cgtcgaggag
     3001 gcccgcgcac ttgctcccgg aggtcgcctg gaccgagtag ctgaaccgcg cggtcgtcgg
     3061 gctccccgcg cacgtcgcgg cgtgcttcac gtagagcgtg taggtcccga acgggaggcg
     3121 gttcccctcg tagacgacgc tctcggtccg gttcccggcc ccgcaggcgg ccggcagcct
     3181 ccgcccgccc tgctgcgcgg cgacgtcgat ccctcccggg ccggcgatcc ggacgtcgag
     3241 gtccgcgtcg cccgaccagc tcgcgagacc ggtcaggtac tggggcgtcc cctgcgcgtc
     3301 gacgcggatc gtggcgacgg acgcgttcgt cgccgagccg cccttcccgt ccgtcacggt
     3361 gagcgagacc gggaacgtcc cggccgactg gaacgtgaag ctcgccgtcg agccggtggc
     3421 cgaaccggag ccgaaattcc agctgtaggt gagcgggtcg ttgtccgggt cggaggccgt
     3481 cgcggtcgcc gttacctgga gcggaacgga gccgtagagc ggcgtcacgc tcgcggagcc
     3541 gatcgtcggc gccgcgttcg tctcgccgcc gctttgggac gcggcgacgg cgacgccggc
     3601 cacgacgacg ccggccccga tgccgaagac cgttccggcc gagaggccgc ccgccttcgc
     3661 cgcagccgcc ccggccgcag cgccggtccc cgaggagagg gcgctcgaga gcgacacgac
     3721 cgccccggtg accaggatca ccttcgcgat gtcgtccttg ttgaagccgg gcggaacagg
     3781 ctcctgcccc gcgcgcgtca gccccacggt gagcccctcg cgcggcgagg cgctcgtgac
     3841 gaccgtggtc gccgcccgct catcccggca gacggtcttg tccgtcacgg ggggtgcgta
     3901 agccggcgtc gtcttgggca ggctcgcgag gtcgagcgcc tggacgtaat agtcgatcgc
     3961 ggggatgccg ggcagcgggc gcggcagctc gccgacgaga tcctccggag cccgcaccct
     4021 catcaggacg tagtagtagt cctccgtccc cgaggcacgg aagtagacga agccctgctc
     4081 cagctccttg cccggaagga cgcgggcgtc cacgagcggc cgcgcctccg tggagaggca
     4141 cttcagcggg tcgtgcgcga tcgagagccg ggggctttcc ccgggcgcgg ccctcttcac
     4201 gaccggttcg gtctccggcg ccggcgcggc gaatgcgccg gtcgagagcg tcgcggccac
     4261 gacgatcgcg aacgcctgtc ggagcggcag cggaagctcg gggagcgcgg ccttctgctt
     4321 cacgggcgga cctcctcggt tcggcaggcg gcccgtcggg cgcgtctcgc cgacgcgcga
     4381 agagacggac ccccggcggg tgtacccggt acgaatcggc tctttcagtt gcaggcgact
     4441 ataacgccga cccgccgcgg cgggtcggtg cgccggatca cgggcagggg ccgatctcga
     4501 gggtccgcga ccaggagtca ccggagccgt ccgtgacctt gatcgtcttg aggaacgagg
     4561 cgcccagggg cccctggaac gcccagaccg gctccttccg tccgacgcgg accttcgccg
     4621 gaggcggcgg ggagacgacc ggacggaagt tcgtgacgct catcccgacg ttctcctcga
     4681 cgtggtcgag cgcgacgtcg cgctccgcct cgttcgagaa gtaggcctct atcgtgacga
     4741 gctgcccctg ctgcgggcgg ctcggccgga cctcgaactc gatgtagaag cggcccaggt
     4801 cgggctttgc ggcccgggcc tgcggtccgc tgccgacatc gaaccccggg gcgaacccgc
     4861 tggtcttccc ttgcgcgaag ctcgtcggcc ccatctggac gcagaggcct ccgccggcgg
     4921 ccggcgtcgg agccggccgc tccacgacga cgcgggccgt cggccgcggt gtcgggacct
     4981 tgacgacgat cggcttcgtc ggctcgacgc gcggcgtcgg cagcggcggg gcctccgtcg
     5041 ggacgacggt cggctccggg aacggcgtcg ccggcgggaa gaccgccgtc ggcgtcggga
     5101 gcgggatacc gggcgtcggg acggcggtcg gctgcgggag aggaggcgtc gcggcgggcc
     5161 gccccaggcc ggggatcagc ccgctcctgt agaggagcgt cccgccgagg ccgacgaaaa
     5221 ggaggccgga gacgacaacg gcgatcggga ggatcgggga gcgcgccggg gtcgccggac
     5281 gcgcggccgg acgcggcggc ccgcgtcgcg cgacgcccgg aggggccggc gccgggggct
     5341 cggcaacggg cggcgcgggc ggcgccgcga tctgcgccgt cgccttcgtc ggttccgggg
     5401 cgaccggagg catcgagatg ccgtgcgcct cgacgtgcgc ccgctccgag gccgcccggg
     5461 cctccgaggt gtcgagggcg acggtcccct cgtccagcga gagctcgccc gccatgccgg
     5521 ccgagagggc ggcagcctgc cggagcgcgg cgtcgcgcag ctccgccatc gtctggaagc
     5581 gcgcctcggg cttcttgttg agggccttca ggacgaggcc gcgcgtgacg agggagatcc
     5641 ccttgttcat gtcgcgcgga tcgggcggct cctgctgcac ctgcatgaac aggacggcgt
     5701 ggctcgactc ggcgacgaac gggacgcgcc ccgtcagcat ccggaagagg acgaccccga
     5761 gcgcgtagac gtcgctccgg cggtcgacct tctgccccat cgcctgctcc ggcgacatgt
     5821 agttcggcga gccgacgagg aagccggtgc gggtcgtccc cccttcgcgg tcgatcaggt
     5881 aggcgaggcc gaagtcggcg accttgaccc gcccttccgc cgtcatcagg acgttcgccg
     5941 gcttgacgtc gcggtggacg atccccttct cgtgggcgta gtggagagcg tcggcgatct
     6001 ccgcgacgat cttcaggcag cgcgactcgg tgaggacttc cttcgagatg aggctgtcga
     6061 ggctctcgcc gtcgacgaac tccatcacga ggtagatgag gttctcctcc tcctcgatgt
     6121 cgtagacctg gacgatgttc gggtggttca gcttcgcgac cgacttcgcc tcgaggcgga
     6181 agcgacggac gccgtccggg tcgccgatca ggtccgacga caggagcttg atggcgacgg
     6241 tccgctcgag ggacggctgg tgggccctgt agacgacgcc catcccgccc cggccgagct
     6301 cggcctcgat gagatacttt ccgagctgct tcctcattgc tcgcccttga acccgatgac
     6361 tgagtgtacg tcagccgctg cgccgtcccg gccctctccc ccgcccccgg gcgaagaggc
     6421 ctgggcacgc gccgccgagg ccgccgtcga ggcgctccgg ctcgacccgc agcgaacgcg
     6481 cgccgggctc ctctgcgtcg ccgcgggagc ggcggtcctc gtcgcgctcg tctccatcgt
     6541 ccagggaggc cgccgcgagc tcctgcgaac ggtcgaggcc gccggaccgt cgaacgtctt
     6601 cgtccgcgcc gagcgcggcg cgccgacgcc gctcacgacg gccggccttc tcgccgcccg
     6661 cgcccgcttc cccggcctgc gcgaagccac cggcgtccgc gtcgtcccgg ccgccgtccg
     6721 cggcgggacc ctcgagctgc cggcgacggt ctacggcgtc ggccccgagg cgctcggcct
     6781 cttcggcctc cgtcgcgcgc ggggccggct cctcggcccg cacgacgccc gctcccgctc
     6841 gcgcaccgcc ctcctcggcg cgcgcctcgc cgccggcctc gcccggcacg gcgacccggt
     6901 cggcacgcgg ctcaccgccg gcggcgagac gtacgaggtc gtcggcgtcc tcgagccctc
     6961 ggcctccgtc gccgggagcg gcggcgagct cgccggcgtc gagtgggact cgggcctcct
     7021 cgttccgctc ggggccgagc cgggcgccgc gaacgccccg gagggggaga tgccggtcga
     7081 cctcctcgcc ctccgcttct ccgacccggg ggaggcggcg cgcgccacgc gcctcctctc
     7141 cccgctcgtc ccctcgggcg cggccgtcac gacgccgacg caggcgatcg agcagtaccg
     7201 cgccgcccat cgctccttcg accgggtcgt cctcctcgtc tccatcctga cggcggcctc
     7261 cgccgccttc ggcgtcacga acctcctccg cgcctcggtt cgggcgcgct cgctcgagat
     7321 cggcctgcgg cgcgccgccg gcgcccggcg cgaagacgtg aggctccagt tcctcgccga
     7381 ggggctcctc ctcggcttcg gggggggcct cctcggcatc ctcctcggcg tcctcctctc
     7441 cgtcacgctc ctctcgcgtg ccggatggac gccccacttc gccccggcca ccctcctcct
     7501 cctcgcgggc ggctcggccg cgttcggcat cgcggccggg atccgtcccg cgaacgaggc
     7561 cgccgcgctc gacccggcgg cgacgctgag gctcgagtga tcgtcccgct cctcctcgcg
     7621 gccgttctct ctgcgccgcc ggcgtccgag ccgctctccg tggaacgggc cgtcgagctc
     7681 gcgctgaccg gcaatcccga gctcctcgac gcggtcgacc gggccgcgct cgccgcggcg
     7741 accctctccg ccgcccgctc gcgctacctc ccgcaggtca ccccctacct cctgccttcg
     7801 ttccgggacg gcgacggcgc cgcggtgagc cgctacggcg tgagcgtcac cgagcagctc
     7861 ccgttcgggc cgcgcctctc cgggaacgtc gaggcgagcc gcctcgaggg ggcggaaggc
     7921 tgggactcct cccactccct cacgctctcc cagcccctcc tgcgcgggct cgacccggcc
     7981 gtcacgcgcg agccgctccg cgcggcccgg cgccaggtcg ccgccgagga gcggaacctc
     8041 ggcgtgcaga ggcggctcac ggtcgtcgcg gtctggagcg cctacctctc cgcggccctc
     8101 caggacgagc tcgtccgcga ggcggccggg cggacgacga gggccgggcg gatcctcgag
     8161 gcgagccgtg cgaaggagga gatcgggagc gtctcccgcc tcgacgtcct ccgggccgag
     8221 cagctcttcg cccaggcccg ctcgcaggag aacgacgcgc gcaacgcccg ggaggacgcc
     8281 cacgacctgc tcgcccgcct cctcggcctc ccggccggga gccgtttccg cctcgagccg
     8341 ccgaccggcc tcccggtcga ggtccccggg ctcgaggagg ccctcgacgc cgcggccgcg
     8401 tcccgcgagg agcttgtcga ggctcgggag cgggtgacgg acgccgaggc gcagctccgg
     8461 atcgcgcgga gcctcctcct cccctcgctc gacgccgggc tcacctggag cgcgtcgggg
     8521 cgcgcggacg gcttcggcgg cgcgttcggc cgtccgggcg gctcgacctg gacgctcggg
     8581 ttcagctcgc aggcgcccct caacctcggg gcgcagctct cggcgcggac ccaggccgag
     8641 gtctccctcc ggatcgccgg gcgagccgtc gaggtcctcg aggccgacgt cgcccgcctc
     8701 gtccgctccg ccggccgccg cctcgccacc tcgcgcgagc ggctcgcgct cgacacggcg
     8761 aacgaggagg tcgcgcggat gcagctcgag gtggccgagc tccgcttcgg caaggggctc
     8821 accgacaact tcttcgtcgt cgacgccgag gggctctaca acgccgcgcg cgtctcgctc
     8881 ctcacctccc gccgcgaggt cctcctcgac gggctccgcc tcctcgccga ggcgggcctc
     8941 ctccgcccgg aggcgttcct cccccggccg gccccctcgc cgtacacttc gtccagacga
     9001 tgatcgaagc cgagtccgtc gcgccggccg tgcgccgctt ccgcgaggcg ttctcccgcc
     9061 tcgcggcgct gccgccgcgg acgaaggcgc tccttctcgg cggcacggcg gtcgccctcc
     9121 tcggggcggg cctggccgtc cgccaggccg tcgtctcgga cgccgtcggc gaggtgcggg
     9181 ccgtgcgcgg gccgctccgg atcacggtcg ccgtcgccgg cgccctcgca cccgcccggg
     9241 ccgagagcta cggacccgag atcccgggcg tcgagctgaa ggtcgtcgag ctcgtcccgg
     9301 aggggacgtg ggtcgcgccc ggcgcgctcc tcgtccgctt cgacgacgga cccttccggc
     9361 gcgagctcga gacggcccgc tcgcgcctcg tccagacgtc gggggaggcc gagcaggcac
     9421 gccaggcgct ccgggccctc ctcgcgacgc agaggagcga gcggctcgag gccgagacgg
     9481 ccttcgcgcg cgccgagctg gacctctcga cgttcgtcaa cggcctcgcg ccgctccagg
     9541 tccagcagtc ggcggccgcg gtcgagcggg cgaagcggga ggccgcggag tcgacgcaga
     9601 agctcgaggg gctcgcgccg ttcgccgaga agggatacgt ctcccgggac gagctgcgcg
     9661 ccgccgagcg ccgggccacc gaggcggcgg ccgacctcgt cctcgccgag cagcagcacc
     9721 ggacgctgac gcgcttcacc cacccgcagc tcctcgccca gaagagccag gaggtggagt
     9781 cccggaggaa cgcgctccgc gtcgtctccg agaagacctc ggcccaggcc gcgcaggcga
     9841 aggccgcggc cgacctggcc agcgcccgcg ccgcggaggc ggcggccgcc gtcgccgacg
     9901 tggagcggaa gatcgccctc tgcaccgtcg aggcccgctc gagcgggctc gcggtccacc
     9961 gcgagatctt cgagaagggg ggcgagaagc ggcgcgtacg cgtcggcgac gccgtcttcg
    10021 ccggccagcc cgtcctcgac ctccccgacc tctcggagat gctcgtcgag acgcgcgtga
    10081 aggagggcga gatccaccgc gtcgcgcccg gccagccggc cgaggtccgc ctcgaggcct
    10141 tccccgacga ggtcttcgcc gcgaccgtcc tccgcctcgg cgccctcacg gcgggcgacc
    10201 gcggcgaggc ccgctccttc ccgctcgtcc tgaagctcgc gaaggccgac ccgcgcctgc
    10261 gccccgggat gaccgcgcag gcgctcgtcc tcgccggcga ggtggccgac gcggtccagg
    10321 tcccgatcga cgccgtccgc tacgagggcg acgcggccgt ctgcaccgtg aagaccgtca
    10381 ccggcgccga gacccgacgc gtgaagaccg gccgcagcaa cgcgttcttc gtcgagatca
    10441 ccgaagggct cgccgagggc gacgtcgtgc tcctcgcgcg gtagggggca aggcgccctc
    10501 ccgcgacccg ggagagccga cgcctccgcg gtcgtcgccc gctgcctata ctcccctccg
    10561 tggaccgcga acgctggagc tcctatcccg ccgcgaccga agaggagcgc gcgctcctcg
    10621 cggtcctgaa cggaacggcc gtacgaagga ccctcccggt cgtctcctcc cgcggcgacg
    10681 agcgctccgt cgaggcgacg ctcgcggtcg aggcggcgga gccgttcgac gggaaggccg
    10741 aggtcgtcgt ccgcctcgtc ttccacgggc ccgcgccggc ggacggcgag cggctcgccg
    10801 aggacccgtg ggacctcctc gacgcgttcc tccgcgaggc cgaaggggag gcgcggcgcc
    10861 gggcttcggc gcggcggtga ccgtccgccg gcgcgggtcg gccgccggcg acccgctcga
    10921 cctctttcac gccgcggtcg ccgcctggtt ccgcgagacc ttccccgaag gcccgaccga
    10981 ggcgcagcgc ctcgcctggc ccgcgatcgc ccgcggcgag agcgtcctcc tcgtctcgcc
    11041 gaccggcacc ggaaagacgc tcgccgcctt cctcgtcgcc ctcgacgcga tcctccggcg
    11101 ccgcctcgcc ggcgacgacg ccccgcgcct tgcgacgctc tacgtctcgc cgctgaaggc
    11161 gctcgacaac gacgtccatc ggaacctcga gcggccgcgc gaggggatcg ccgcgcagct
    11221 cggggcgggc cgcgtcccga tcacgacggc cgtgcggacg ggcgacaccc cgtcgcgcga
    11281 gcggacgagc cagctcaagg cgccgcccga cgtcctcatc acgacccccg aatcgctcta
    11341 cctgatgctg accggcggcg ggcggcgtgc gctcgagggg gtctcgaccg tcgtcgtcga
    11401 cgaggtccac tccgtcgccg ggacgaagcg cgggagccac ctcgccctct cgctcgagcg
    11461 gctcgaggag gtgatcgcgc gcgccggcgg cgccccgccg cagcgcgtcg ccctctcggc
    11521 gacggtcgcg cccgtcgacg tcgtcgcctc gttcgccgcc gggacgggcc ggaagatgaa
    11581 tccgctggtc gtcccggcga agaaggcgct cgacctcgtc gtcgcctcgc ccgcccccga
    11641 cttccgcgag cttcccgacg gctcggcgtg ggggccggcc gtcgaggcgg tcgtgaagga
    11701 ggtcgagtcg cggaagacga cgctcgtctt ctgcaacaac cgcaggctcg ccgagaaggt
    11761 ctcgcgccgg ctctccgacg cgaccggcgc cgacgtcccg acgcaccacg gctcggtctc
    11821 tcgcccgcgg cgggaggaga tcgagaagga gctgaaggcg gggacgctcc ccgtcctcgt
    11881 cgcgacgggc tcgctcgagc tcgggatcga cgtcgggcac gtcgactccg tcgtcctcct
    11941 cgagagcccg aaggggatcg cgcgggggct ccagcgcgtc ggccggtcgg ggcacctcgt
    12001 cggcgagacg gcgcgcggcc tcttcgtccc gctctacctc gacgacctct tcgagagcgc
    12061 ggcgacggcc gaggcgatga gggagggggc cgtcgaggag acgcgcccgc ccgacttccc
    12121 gctcgacgtc ctcgcgcagc agctcgtcgc cgaggcggtc gcgcgcgcgg ccgacggcct
    12181 cgccgtcacc tccgccgagc tcttcgccct cgcgcggaag gcgtacccgt accgggcgct
    12241 cccgcgctcg ctcttcctcg agacgctcgc gatgctctcg gggaagtacc cgcgcgagcg
    12301 cttcaaggag ctcgcgccga agctcgtctg ggaccgcgcg acggaccgcg tcaccccgct
    12361 ccccggcgcc cgcatcgccg cgctcctcga cggcggcacg atcggcgacc gcggaacgtt
    12421 ccgcgccgtc ctccccgacc ggaagacgct cgtcggcgag ctggacgagg agttcgtcca
    12481 cgagacgaag gagggggacg tcttcctcct cggctctcgc tcctggcgcg ccgtcgagat
    12541 cggccacagc gccgtcgtcg tcgaggaggc cgcgggactc cccgcgccgc ggatgccgtt
    12601 ctggaggggc gaggggctcg ggcggacggg ggacctcggc gagcggatcg gacggctccg
    12661 gcgcttcgtc gccgagcggc tcgacgaccc ggggctgaac gaggccgtcg cgacacgcta
    12721 cgcgacgacg cccgacgcga cggccgcgat cgtcggggcc gtacggcgcg aggcgacgga
    12781 gtcgggcggc ctcgcctccg acctcgccgt cgtcttcgag acgttcccga acgacctcgg
    12841 cgacccgtgc atcgtcgtga ggtccctctt cggccgcggc gtgaacctcc cgtggtcgct
    12901 cgtcctctcc gccgtcctcc gcgaggagac gggggtggac gtcgagacgg tcgcctccga
    12961 cgacgggatc ctcctccgga tcccgggggc cgagcgcgag gtgccgctcg agcgcctctc
    13021 gcggatctcc cccgccgagg cgaaggagcg gctcctcgcg cagctcccga actcgccgat
    13081 gttcggggcg cgcttccgcg agaacgc
//
LOCUS       DAOTEZ010000068        13101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3306_length_13101_cov_6.225663, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000068 DAOTEZ010000000
VERSION     DAOTEZ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13101
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3306_length_13101_cov_6.225663"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="P5164_05380"
     CDS             complement(<1..712)
                     /locus_tag="P5164_05380"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRY43361.1"
                     /translation="MALTTRVLPPPKLARFMERHRLLDETSGIAPERFLVTLVKKALD
                     FVPAEAAVLLLDDPVAKEEDRSRNELTVIASAGDIRDPAVGTRVRPVDGIAGFTYARG
                     EQTAVNEIKGARALSREADEVFGSPVRNALGVPVVIESHVCGSLVLVNRIEPAGFGAL
                     DERLMALFGETLSLALQNLLDERRAREVARRDSLTALYNDRYFHKKLRQEIDRLSQTP
                     DGDLTLLFLDCDHLKAVND"
     gene            complement(722..2575)
                     /locus_tag="P5164_05385"
     CDS             complement(722..2575)
                     /locus_tag="P5164_05385"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HRY43362.1"
                     /translation="MSRAARSAALLAPLAALGLVFAATPLHAAQEGPEVRVVVDNPRP
                     AADDVVRLTYVFSGPGIGGRLHAPAELPLRNLVALGPPSSATQATLINGVFSRTSSLT
                     YFLRPTGTGPAEIGETIFRFGEKEVKAGAYLLEVSPARRESAPDAAAPPREEDPFDRV
                     FPRRGTRPAGVERGLPSAEPLLAFVATPSRETAFVGEEVTISYDLVTEADVQGIEYVD
                     APQFPGLWAEDLERPERPQGRKDTYDGRPVARFTLLRKAVSGLTPGTVTIPASKIRIG
                     VRVALDPFGDPFAMLRPRVLERSTKPITLKVLPIPGRPDFKGPVGQFGVTASVDRKRV
                     PAGEAVTLKVRLAGTGNLRTATETPHLSIPGVKVYPPSSRTLPSRGGTKGGTSAEWDF
                     VLVPTEAGSLVVPPVSFEVFDTAEKKVVARSTAPISITVEAAAPGAVIAEGPPPASDA
                     ATGAEAATTLPGAPPTPTPLPSARELAQSTVAVPLWVLVAIPGLLAVAGGATWAVRRR
                     GRALGPAAALLAPEPGETKERAVARVERAVRGHLARRTGVAETAPVGDLLEELAGRGV
                     DPELREELRQLLAQVEFLRFAPQLGDYSDELARLRDRAREVLGRVRDGRRG"
     gene            complement(2572..4329)
                     /locus_tag="P5164_05390"
     CDS             complement(2572..4329)
                     /locus_tag="P5164_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRY43363.1"
                     /translation="MKELFLSPAWLWATLGGALLAATSAWLSARRVRRRREGLVAPAL
                     AEKAGLLSRDRWTPFAALLAVLATLGTGLALARPRWGLVTETVERRGADVVVLLDTSA
                     SMRATDVTPSRFVLARQAALSLAEKLPGDRLALVGCEGEAQVLVPLTLDTAAVALFLE
                     ALEPGIGALPGTSLAAGLSAAAELMPPGTSAGRQVVVLSDGEDLEGGVDEAISKAKSE
                     GIVVHGVFVGVEGRGAPVPEVDVAGRPSGFKTQEGAPVLSRPDPGLLRRLAAETGGSF
                     SVVAPGRTDLDGVAREIDKAARRPLSGSVGTNRRERFQIPLAVAVGALALLLIGPLGF
                     LRPRRARSGVAAALFLLLSGPSSGRPLLAQSPPVAPAGTGAPSGPAVAPTPASLKERV
                     LSRPPFTTARGEAVAGKKALEEKRLDEAVARFAREAALAPKDSAGSYNLGTALALAGR
                     ADEALAALKKAKGASRGDVAADASHNAGTVLLSGRQWEAAAAAFRDALRARPGDADAA
                     FNYELALRRLEQQKQQQRQQQGGGSPPPPKKDDFEKKAKMSRDKAEQLLQAIARNDLE
                     EQRKKVAEQTKKRRPGRDW"
     gene            complement(4326..5369)
                     /locus_tag="P5164_05395"
     CDS             complement(4326..5369)
                     /locus_tag="P5164_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRY43364.1"
                     /translation="MSLLATLLPPGLTFRDPLVLLLLLVVPVAVALRIARERRGTSAL
                     VVPTLAFVAKVKPGWRVRFRHLPFVLACLGFAGLVVALARPRVGLERTESWTEGVDIV
                     VALDASGSMAAEDFKPKNRFFVAKAATRTFVEGRPQDRLGLIAFAGRSRTVVPLTTDR
                     DMLLQRLGALSLGEQGDGTAIGMALGNALARLGASKAKSKVIVLVTDGGNNAGEIDPD
                     TAANVAKGLGVRVYTIGVGTAGGPVEIPVPVRDPETGRVVERRVMAEVEVDEKLLGRI
                     ADATGGRFFRATDPEGLARTYAEIDRLEKTEVKTTTYTRWRETFPRVAFPAAGLLAAA
                     ALLGLLAFPVAPR"
     gene            complement(5366..6472)
                     /locus_tag="P5164_05400"
     CDS             complement(5366..6472)
                     /locus_tag="P5164_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43365.1"
                     /translation="MRIVALLATAAALAAPLAAQAPRPGLPEATVPPPLVVTSAADRT
                     TAAVGEPVTLVYSARIPEGAELRLTHLVSPPRPEGQPATAGFVLDFEPVGPSVAERVK
                     GDAGHVDVRQTVRVAAFVPGETRVPGPVFAYRAPDGTTAVLRAPGVALTIASRLPEGE
                     DSEALAPKPARPVRVPARSPWFWASIAAVVLALAGLVTWLVKRRKKGATAGAAAAPPP
                     VPPDVELESALAALAARAASLDGDPRPFYSDLTHAAKRFLERHLGEPVLEWTTFETVR
                     RLRDLGAEPPREVALADLLMGADRVKFGRAGATAAEAAGALEGARHLLAWGRTRMAAQ
                     AAAEREAAKAAAKAPRPPVAAKGTPTARPAGGAR"
     gene            complement(6469..7353)
                     /locus_tag="P5164_05405"
     CDS             complement(6469..7353)
                     /locus_tag="P5164_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRY43366.1"
                     /translation="MLPREIVKKIQRIRITTNRMVDAGLGGEYHSVFRGRGMEFSEVR
                     EYVPGDDVRAIDWNVTARTGVPHVKKFVEERDLTTLLVIDLSRSQEFGSRFLTKRSLM
                     AELAGLLAFAAVKNNDRVGALLFTDRLERFVPPRKGFDHALSILRDVLVQPMEGTRTD
                     LGLALKTAASLLKQRSVVFVLSDFLAAGYEKPLKILRRRHDVVAMPITDPLETAIPDR
                     GLVRLRDAETGLARVFDAGDPAFRAAWKRRGGPFPDPAEVFRASGIDSVPLTTDAPYE
                     RALVRFFKERERRRSAGR"
     gene            complement(7438..8448)
                     /locus_tag="P5164_05410"
     CDS             complement(7438..8448)
                     /locus_tag="P5164_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRY43367.1"
                     /translation="MSIAPADTAALTEKIRESSLAVTALLAEVRKVIVGQPKMVERLV
                     IGLLADGHVLLEGVPGLAKTLAVRTLARGLDASFSRIQFTPDLLPADLVGTLVFNPKT
                     QEFVARRGPVFAHVLLADEVNRAPAKVQSALLEAMQERQVTIGDETVPLPKPFLVLAT
                     QNPIEQEGTYPLPEAQVDRFLMKLKVGYPTKAEERQILDRMGDATEKTASDSVRPVLT
                     PAQIVELQALVRQLYADERIKGYLVELVAATRDPRAYGLPELADFVQYGASPRATLAL
                     LSTARAHAFLQGRGYVVPEDVKEVAPEVLRHRVLLSYEAEAENVSSDQVVSRILERIE
                     VP"
     gene            8598..9002
                     /locus_tag="P5164_05415"
     CDS             8598..9002
                     /locus_tag="P5164_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43368.1"
                     /translation="MRRPLVPFLVAALLALALVVPGPADAATKRRKKKVRRPVPTPTE
                     RPYYGPPAPTPAPWRRAAGACMEYTPGQYLVVAEVGATGRVFRVDGETKIETEVRKGL
                     RVRILYVDRPEGPVARRILPGPIAATPSPRTP"
     gene            complement(9126..9455)
                     /gene="trxA"
                     /locus_tag="P5164_05420"
     CDS             complement(9126..9455)
                     /gene="trxA"
                     /locus_tag="P5164_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011460330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRY43369.1"
                     /translation="MAGNVQEFTAANFDEAVLKSGTPVLVDFWAVWCGPCRQVAPVVE
                     ALAAKWGATVKVGKVNVDDHPSIAEKYNIMSIPTLMLFKDGVVVERVIGFASQAALEQ
                     KFQQHVA"
     gene            9531..10340
                     /gene="rsmA"
                     /locus_tag="P5164_05425"
     CDS             9531..10340
                     /gene="rsmA"
                     /locus_tag="P5164_05425"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRY43370.1"
                     /translation="MRASRRPRLGQNFLSSPRTAGRIVDALGAPAGSWVVEIGPGKGA
                     LTRPLLARGLRVLAVEVDAGLAERLRAGLGGEDLRVVEADALAIDPAAALAEAGATPP
                     VPLVANLPYESATPMVRAFARRRDLFSRLVVMVQREVAERICGRPGDDGYGFLSVDVA
                     FHAAARRLFDVPPGDFSPRPKVVSTVVELVPAVPPEDAAAAIAVASAGFGVRRKTLVN
                     GLTAAWGREAASAAVASAGLLPTVRAEELPPEGFLALARRLGPRPAAAGTR"
     gene            10380..11216
                     /locus_tag="P5164_05430"
     CDS             10380..11216
                     /locus_tag="P5164_05430"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRY43371.1"
                     /translation="MSVTFWQAILLGILQGLTEFLPVSSSAHLILAQRLLPGFSQPGI
                     LFDVMLHVGTLVAVVVYFRERIGSLLAGAVSRDPAVRRPAWKIAMLLATSVALTGVVT
                     LPLKKVALEGMESLPKIGLALLATSALLAAAQAIGRRRGEGGRTLEEMRFFDAALIGA
                     FQSLSALFRGLSRSGNTIAMGLFTGLSRRAAAEYSFLLSVPTILSAALVENVSEYRAS
                     GHLVTEGSHLGVYLAGMAAAAVVGYAAVALLLRLVVAMKLQAFVLYCALLGAVVLAAG
                     LS"
     gene            11222..>13101
                     /locus_tag="P5164_05435"
     CDS             11222..>13101
                     /locus_tag="P5164_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HRY43372.1"
                     /translation="MTEVPRSPETPHRRRDEFLGILLVAAGLLLVASLFSYDPADPSL
                     FSAVADPDARPGNWAGRVGASFADGALQFFGFAAFALPLVVLALGMARFRSRAVGAWG
                     TKALGVVVVLLSSAPLCHLAFGRPRLLGGGLGAGGFVGDLVAGALVAAFNPPGAAIAL
                     SAALLIGLLLVASLSLGEAARRVALRTSASLAAWRLERERRRQVEEKERTRRAVVKKH
                     LERALEEPPPADEEDEAGGEVVAIPSLATLRVRERSGAGQFSVRRTAGPPVAAGAETG
                     VAASCLPVPASPGRPAPPGRPSPVARPAQLKKTEPAPQRVLPFPSPKESNGWVFPPEK
                     LLRPAPKRDRDKTREEFQQTMELIAAKCLEFGVEGAVDSYRPGPVVTTYEFKPSAGVK
                     VAQVAGLENDLALALAAEKVRIERIPGRAAIGIEVPNRNRDLISLREIVESERFKRSP
                     SLLTIALGIDVEGNPVVADLARMPHLLVAGMTGAGKSVGVNGMITSILYKARPDEVKF
                     IFVDPKMNDMKDYDDLPHLLVPVVVDPKKAANALKWAVDEMEGRYKLLSEWPGVRNIE
                     QYNAAIRDPEAVAEVREKLGEKLKTDGPDGTISPMPYIVVVIDELADLMMTAPKDIEE
                     SVA"
BASE COUNT         1700 a         4693 c         4948 g         1760 t
ORIGIN      
        1 cgtcgttcac cgccttgagg tggtcgcagt cgaggaagag gagcgtcagg tcgccgtcgg
       61 gcgtctgcga gagacggtcg atctcctggc ggagcttctt gtggaagtaa cggtcgttgt
      121 agagcgccgt gagcgagtcg cggcgcgcca cctcgcgggc gcgcctctcg tcgaggaggt
      181 tctggagcgc gagggaaagc gtctcgccga agagggccat gaggcgctcg tcgagcgcgc
      241 cgaagcccgc cggctcgatc cggttcacga ggacgagcga cccgcagacg tgcgactcga
      301 tgacgacggg gacgccgaga gcgttcctca ccggcgaccc gaacacctcg tccgcctcgc
      361 gggagagggc gcgggctccc ttgatctcgt tcaccgcggt ctgctccccg cgcgcatagg
      421 tgaagccggc gatgccgtcg acgggcctca cccgcgtccc gacggccggg tcgcggatgt
      481 ctccggccga ggcgatcacg gtcagctcgt tccgggaccg gtcctcctcc ttcgcgaccg
      541 ggtcgtccag aaggaggacg gcggcctccg ccgggacgaa atcgagcgcc ttcttgacga
      601 gggtgacgag gaaccgctcg ggggcgatgc cgctcgtctc gtcgaggagg cgatgtcgct
      661 ccatgaagcg cgcgagcttc ggaggcggga ggacccgcgt cgtcagggcc acgaggcgat
      721 tctagccgcg ccgaccgtcc cggacccggc cgaggacctc gcgggcccga tcccgcagcc
      781 gggcgagctc gtcggagtag tcgccgagct ggggcgcgaa gcgaaggaac tcgacctgcg
      841 cgaggagctg ccggagctcc tcccggagct cggggtcgac cccgcggccg gcgagctcct
      901 cgaggaggtc gccgacgggc gcggtctcgg cgacgcccgt cctccgcgcc aggtgcccgc
      961 ggacggcccg ctcgacccgt gccaccgccc gctccttcgt ctctcccggc tccggcgcga
     1021 gcagggccgc ggccgggccg agggcgcgcc cgcggcgacg gacggcccag gtcgcgcccc
     1081 ccgcgacggc gaggaggccc gggatcgcga cgaggaccca gagggggacg gcgacggtcg
     1141 actgcgcgag ctcgcgcgcc gaggggaggg gcgtcggcgt gggcggcgcg cccgggaggg
     1201 tcgtcgcggc ctccgctccg gtcgcagcgt cggaggcggg cggcgggccc tcggcgatca
     1261 ccgcgcccgg agcggccgcc tcgaccgtga tcgagatcgg ggcggtcgac cgggcgacga
     1321 ccttcttctc ggcggtgtcg aagacctcga acgagaccgg gggaacgacc aggctgccgg
     1381 cctcggtcgg cacgaggacg aagtcccact cggcgctcgt cccgcccttc gtcccgccgc
     1441 gggacggcag ggtccgcgac gagggcgggt agaccttcac gcccgggatc gagaggtggg
     1501 gggtctcggt cgcggtccgg aggttcccgg tgccggcgag gcggaccttg agcgtgacgg
     1561 cctcgccggc cgggacgcgc ttcctgtcga cgctcgcggt gacgccgaac tgaccgaccg
     1621 ggcccttgaa gtcggggcgg ccggggatcg gcaggacctt cagcgtgatc ggcttcgtcg
     1681 atcgctcgag gacgcgcggc cggagcatcg cgaacgggtc gccgaacggg tcgagcgcga
     1741 cgcggacgcc gatccggatc ttcgatgccg ggatcgtcac ggtgccgggg gtgaggccgg
     1801 agaccgcctt gcggaggagc gtgaagcgcg cgacggggcg cccgtcgtac gtgtccttcc
     1861 gcccctgcgg gcgctcgggc cgctcgaggt cctcggccca gaggccgggg aactgcgggg
     1921 cgtcgacgta ctcgatcccc tgaacgtcgg cctcggtgac gaggtcgtag gagatcgtca
     1981 cctcctcgcc gacgaaggct gtctcccggc tcggggtcgc gacgaaggcg aggagcggct
     2041 cggcggaggg gaggccgcgc tcgactccgg cggggcgggt cccacgcctc gggaagaccc
     2101 gatcgaacgg gtcctcctcg cgcggagggg cggccgcgtc gggggcggat tcgcggcgcg
     2161 cgggactcac ctcgaggagg taggcccccg ccttcacctc cttctcgccg aaacggaaga
     2221 tcgtctcgcc gatctcggca ggacccgttc cggtggggcg aaggaagtag gtgaggctcg
     2281 aggtgcggga aaagacgccg ttgatgaggg tggcctgggt cgcgctgctc ggcgggccga
     2341 gggcgacgag gttccggagc ggcagctcgg ccggagcgtg gaggcgccct ccgataccgg
     2401 ggccggagaa gacatacgtc agccggacga cgtcgtcggc cgcggggcgc gggttgtcga
     2461 cgacgacccg gacctcgggc ccctcctgag cggcgtggag cggggtcgcg gcgaaaacga
     2521 gaccgagcgc cgcgaggggc gcgaggagcg cggcggagcg cgcggcgcgg ctcaccagtc
     2581 cctcccgggg cgccgcttct tcgtctgctc ggccaccttc ttcctctgct cttcgaggtc
     2641 gttccgggcg atcgcctgga ggagctgctc ggccttgtcg cggctcatct tcgccttctt
     2701 ctcgaagtcg tccttcttcg gcggcggcgg cgagccgccc ccctgctgct gtctctgctg
     2761 ctgcttctgc tgctcgagcc gccggagcgc gagctcgtag ttgaacgcgg cgtccgcgtc
     2821 gccgggccgg gcccggagcg cgtcgcgaaa cgcggccgca gccgcctccc actgcctgcc
     2881 ggagaggagg acggtcccgg cgttgtgaga ggcgtcggcg gcgacgtcgc cccgcgaggc
     2941 ccccttcgcc ttcttcagcg cggcgagggc ctcgtcggcg cggccggcga gcgcgagcgc
     3001 ggtcccgagg ttgtagctgc cggcggagtc cttcggggcg agggccgcct cgcgggcgaa
     3061 ccgggccacc gcctcgtcga ggcgcttctc ctccagcgcc ttcttccccg cgacggcctc
     3121 gcctcgcgcc gtcgtgaacg gcggtcggga aaggacccgc tctttcagcg acgccggggt
     3181 cggcgcgacc gcgggaccgg acggcgcgcc ggttcccgcg ggcgccacgg gcgggctctg
     3241 ggcgaggagc ggacggcccg acgaagggcc ggagagcagg aggaagaggg ccgcggccac
     3301 gccgctccgc gcacggcgag gccggaggaa cccgagcggg ccgatcagga ggagcgcgag
     3361 cgcgccgacg gcgacggcga gcgggatctg gaaacgttcc cgcctgttcg tcccgacgct
     3421 cccggaaagg ggacgccgcg cggccttgtc gatctcgcgg gcgacgccgt cgaggtcggt
     3481 cctcccgggg gcgacgacgg agaagctccc ccccgtctcg gcggcgagcc gccgcaggag
     3541 gcccgggtcg gggcgtgaga ggaccggagc gccctcctgc gtcttgaatc ccgacggccg
     3601 gccggcgacg tcgacctcgg ggacgggcgc gccgcgcccc tcgaccccga cgaagacgcc
     3661 gtgaacgacg attccctcgc tcttcgcctt cgagatcgcc tcgtcgacgc ccccttcgag
     3721 gtcctcgccg tcggagagga cgacgacctg gcgtccggcg gacgtgcccg gcggcatcag
     3781 ctcggcggcg gccgagaggc cggctgccag cgaggtgccg gggagcgcgc cgatccccgg
     3841 ttcgagcgcc tcgaggaaga gcgccaccgc ggccgtgtcg agcgtgagcg ggacgaggac
     3901 ctgcgcctct ccctcgcagc cgacgagagc gagacggtcg cccgggagct tctcggcgag
     3961 ggagagcgcc gcctgtcgcg cgaggacgaa ccgcgacggg gtgacgtcgg tcgctcgcat
     4021 cgaagcagag gtgtcgagga ggacgacgac gtcggcgccg cggcgctcga ccgtctcggt
     4081 gacgaggccc cagcgggggc gggccagcgc gaggcccgtg ccgagcgtcg cgaggaccgc
     4141 gagcagggcc gcgaaagggg tccaccgatc gcgcgagagg agccccgcct tctcggcgag
     4201 ggccggcgcg acgagcccct cccgccggcg gcggacgcgg cgcgcggaga gccacgcgga
     4261 cgtcgcggcg aggagggccc cgccgagggt cgcccagagc caggccgggg agaggaagag
     4321 ctccttcatc gcggggccac cgggaacgcg aggagcccga ggagcgcggc cgcggccagg
     4381 agtccggccg cggggaaggc gacgcgcggg aacgtctcgc gccagcgggt gtaggtcgtc
     4441 gtcttcacct cggtcttctc gaggcggtcg atctcagcgt aggtccgggc caggccctcg
     4501 gggtcggtcg cgcggaagaa gcggccgccc gtcgcgtccg cgatccgccc gaggagcttc
     4561 tcgtcgacct ccacctcggc catcacgcgg cgctccacga cgcgcccggt ttcggggtcg
     4621 cggaccggga ccgggatctc gaccggcccg ccggccgtgc cgaccccgat cgtgtagacg
     4681 cggactccga gccccttcgc cacgttcgcg gcggtgtcgg ggtcgatctc gccggcgttg
     4741 ttcccgccgt cggtcacgag gacgatcacc ttcgatttcg ccttcgaggc gccgagccgc
     4801 gcgagggcgt tgccgagcgc catcccgatc gcggtcccgt ccccctgctc ccccaggctc
     4861 agcgcgccga gtcgctggag gagcatgtcg cggtcggtcg tcagcggcac gacggtccgc
     4921 gaccggcccg cgaacgcgat cagcccgagc cggtcctggg gcctcccctc gacgaaggtc
     4981 cgcgtcgcgg ccttcgccac gaagaagcgg ttcttcggct tgaagtcttc cgcggccatc
     5041 gagccggacg cgtcgagggc gacgacgatg tcgaccccct ccgtccacga ctcggtcctc
     5101 tcgagcccga cccgcgggcg tgcgagggcg acgacgaggc ccgcgaagcc gaggcaggcg
     5161 aggacgaacg ggaggtggcg gaaacggacc cgccaccccg gcttcacctt cgcgacgaac
     5221 gcgagggtcg ggacgacgag ggccgaggtc cccctccgct cccgagcgat ccggagcgcg
     5281 acggccaccg ggacgacgag caggaggagg aggacgagcg gatcgcggaa cgtgagaccg
     5341 ggcgggagga gcgtcgcgag gaggctcatc gcgcgcctcc cgccggccgc gcggtcggcg
     5401 tgcccttcgc cgcgaccgga ggcctggggg ccttcgcggc ggccttcgcc gcttcccgct
     5461 ccgccgcggc ctgcgcggcc atgcgcgtcc ggccccaggc gaggagatgg cgggctcctt
     5521 cgagcgcccc cgcggcttcc gcggccgtcg cgcccgcgcg gccgaacttg acgcggtcgg
     5581 cccccatgag gagatccgcg agcgcgacct cccggggcgg ctcggcgccg aggtcgcgga
     5641 ggcggcggac cgtctcgaac gtcgtccact cgaggaccgg ctccccgagg tgcctctcga
     5701 ggaagcgctt cgcggcgtgc gtcaggtcgg aatagaaggg gcgcgggtcg ccgtcgagcg
     5761 aggcggcgcg cgcggcgagg gccgcgagcg cggactcgag ctcgacgtcg ggcggaacgg
     5821 gagggggagc ggccgcggcc cctgccgtcg cccccttctt ccggcgcttc acgagccacg
     5881 tgacgagccc ggccagggcg aggacgaccg cggcgatcga ggcccagaac caggggctcc
     5941 gggcgggaac ccggacgggg cgggccggct tcggggcgag cgcctcggag tcctccccct
     6001 cggggaggcg agaggcgatc gtgagcgcga cgcccggcgc gcggaggacc gccgtcgtgc
     6061 cgtcgggggc ccggtacgcg aagacgggac cggggacgcg cgtctcgccc gggacgaacg
     6121 cggcgacgcg gacggtctga cgaacgtcga cgtggccggc gtctcccttc acgcgctccg
     6181 ccactgaggg tccgaccggc tcgaagtcga ggacgaaacc ggcggtggcg ggctggccct
     6241 cgggcctggg cggcgagacg aggtgcgtca gccggagctc ggccccttcg gggatgcgcg
     6301 cggagtagac cagcgtcacc ggctcgccga cggcggcggt cgtccggtcg gcggcggagg
     6361 tgacgacgag cggaggaggc accgtcgcct cggggagtcc cggacggggc gcctgcgccg
     6421 ccagaggggc ggcgagcgcc gcggccgtcg cgaggagcgc gacgatcctc atcgtccggc
     6481 cgacctccgc cgctcgcgct ccttgaaaaa gcggacgagc gcgcgctcgt agggcgcgtc
     6541 ggtcgtgagg gggacggagt cgatgcccga ggcgcggaag acctccgcgg ggtccgggaa
     6601 cgggccgcca cgcctcttcc aggcggcgcg aaaggcgggg tcgcccgcgt cgaagacgcg
     6661 cgcgaggccc gtctcggcgt cgcgcagccg cacgagcccg cggtcgggta tcgcggtctc
     6721 gagcgggtcg gtgatcggca tggcgacgac gtcgtgccgc cgccggagga tcttcagcgg
     6781 cttctcgtag ccggcggcca ggaagtcgga gaggacgaag acgaccgagc gctgcttcag
     6841 gagcgaggcg gccgtcttga gggcgaggcc gagatccgtc cgtgtcccct ccatcggctg
     6901 aacgaggaca tcccgcagga tcgacagggc gtggtcgaac ccctttcgcg gcgggacgaa
     6961 ccgctcgagc cggtcggtga agaggagcgc tccgacccgg tcgttgttct tcaccgccgc
     7021 gaacgcgagg aggccggcga gctcggccat cagcgagcgc ttcgtcagga agcgcgagcc
     7081 gaactcctgg gagcgcgaga ggtcgatgac gaggagcgtc gtcaggtcgc gctcctcgac
     7141 gaacttcttc acgtgcggga cgcccgtccg ggccgtgacg ttccagtcga tcgcccggac
     7201 gtcgtcgccc gggacgtact cgcggacctc cgagaactcc attccccgcc cgcggaagac
     7261 ggagtggtac tccccgccga gcccggcgtc caccatccgg ttcgtcgtga tccggatccg
     7321 ctggatcttc ttgacgatct cgcggggcag catcgccgga attccttcag gcgcccttga
     7381 ggtcggcccc gatccccctc gggggggccg gggggatcga gaaacccccg ggaaaactca
     7441 tggcacttcg attctctcca ggatcctcga gacgacctgg tcgctcgaga cgttctcggc
     7501 ctcggcctcg taggagagga ggacgcggtg gcggaggacc tcgggggcga cctccttgac
     7561 gtcctcgggg acgacgtagc cgcgcccctg gaggaacgcg tgggcgcggg ccgtcgagag
     7621 gagcgcgagc gtggcgcggg gagacgctcc gtactggacg aagtcggcca gctccggcag
     7681 tccgtaggcc cgcggatcgc gggtggccgc gacgagctcc acgaggtagc ccttgatccg
     7741 ctcgtcggcg tagagctggc ggacgagcgc ctggagctcc acgatctggg cgggggtgag
     7801 gacggggcgc acggagtcgg aggcggtctt ctccgtcgcg tcccccatcc ggtccaggat
     7861 ctggcgctcc tcggccttcg tcgggtagcc gaccttcagc ttcatcagga agcggtcgac
     7921 ctgggcctcg gggagcgggt aggtcccctc ctgctcgatc gggttctgcg tcgccaggac
     7981 gaggaacggc ttcggaagcg ggaccgtctc gtcgccgatc gtcacctggc gctcctgcat
     8041 cgcctcgagg agggccgact gcaccttcgc gggcgcccgg ttcacctcgt cggcgaggag
     8101 gacgtgcgcg aagaccggcc cgcgccgcgc cacgaactcc tgcgtcttcg ggttgaagac
     8161 gagcgtcccg acgaggtcgg cgggaaggag gtcgggcgtg aactggatcc gcgagaagct
     8221 cgcgtcgagc ccgcgggcga gcgtcctcac ggcgagcgtc ttcgcgaggc ccgggacgcc
     8281 ctcgaggagg acgtgcccgt cggccaggag gccgatgacg agcctctcga ccatcttggg
     8341 ctggccgacg atgaccttgc ggacctcggc gagcagggcg gtgacggcga gggagctctc
     8401 ccggatcttc tcggtgaggg cggccgtgtc ggcgggggcg atgctcatgc ttcctcgggg
     8461 cgcgtcaacg agacgcgcgg acggacgttc ggcggacaga acgctgccgt tctggcgcgc
     8521 caccgggcca accgattctc atcgagcaac gccggtgccg cgacggaacg gcacgcctcg
     8581 gctattctcg gcccccgatg cgccggcccc tcgtaccctt cctcgtcgcg gcgctcctcg
     8641 ccctcgcgct cgtcgttccg gggcctgccg acgcggcgac gaaacgccgg aagaagaagg
     8701 tccggcggcc cgtcccgacc ccgacggagc gcccctacta cggcccgccc gcaccgacgc
     8761 cggcgccctg gcggagggcc gccggggcgt gcatggagta cacgcccggg cagtacctcg
     8821 tcgtcgccga ggtcggcgcg acggggcgcg tcttccgggt cgacggcgag acgaagatcg
     8881 agacggaggt ccggaagggg ctgcgggtgc ggatcctcta cgtggaccgg cccgaggggc
     8941 cggtcgcgcg gaggatcctg ccggggccga tcgcggcgac gccgtcgccg cggacgccct
     9001 gaacgggcgg acccgccggt gaggcgggcc cgcccgcgct cggggcatga gagggaatcg
     9061 gccgaccgtc agtcgccacg gccttggggg gtccgggggg tcgcgatgag caccccccgg
     9121 agagttcagg cgacgtgctg ctggaacttc tgctcgaggg cggcctggga ggcgaagccg
     9181 atgacgcgct ccacgacgac gccgtccttg aaaagcatga gggtcgggat cgacatgatg
     9241 ttgtacttct cggcgatcga cgggtggtcg tcgacgttga ccttcccgac cttgaccgtc
     9301 gcgccccact tggcggcgag ggcctcgacg accggggcca cctggcggca cgggccgcac
     9361 cagacggccc agaaatcgac gaggacgggc gttccgctct tgaggaccgc ctcgtcgaag
     9421 ttggcggcgg tgaactcctg gacgtttcct gccatacggg ttcctttcta ccggcgggac
     9481 gacggacgcc gcgaggccat ccgccgggcg cgcatggtag gttggcgttc gtgagagcgt
     9541 caaggcggcc tcgtctcggc cagaacttcc tctcgagccc gcggaccgcg ggccggatcg
     9601 tcgacgcgct cggggctccc gccgggagct gggtcgtgga gatcgggccg gggaagggcg
     9661 cgctgacgcg gccgctcctc gcgcgcggcc tcagggtcct cgccgtagag gtggacgccg
     9721 gcctcgccga gcgcctgcgc gctgggctcg gcggggagga ccttcgggtc gtcgaggccg
     9781 acgcgctcgc catcgacccc gccgcggccc tcgccgaggc cggggcgacg ccccccgtgc
     9841 cgctcgtcgc gaacctcccg tacgagtcgg cgacgccgat ggtgcgggcg ttcgcccgga
     9901 ggcgggacct cttctcgcgc ctcgtcgtca tggtgcagcg cgaggtcgcc gagaggatct
     9961 gcggccggcc cggagacgac ggctacggct tcctctccgt cgacgtcgcc tttcacgccg
    10021 cggcgcgccg cctcttcgac gtccctccgg gcgacttctc gccgcggccg aaggtcgtct
    10081 ccaccgttgt cgagctcgtg cctgccgtgc ctccggagga cgccgccgcc gcgatcgccg
    10141 tggcgtcggc ggggttcggc gtacggcgga agacgctcgt caacgggctt accgccgcct
    10201 gggggcgcga ggccgcgtcc gccgcggtgg cgtcggccgg gcttctcccg acggtccggg
    10261 cggaggagct tccgcccgag ggcttcctcg cgctcgcccg gcgcctcggg ccccggccgg
    10321 cggcggccgg gacccgctga atccgcggag cggcgctccc gggctgctaa acttcgtcga
    10381 tgtcggtcac gttctggcag gccatcctgc tgggcatctt gcagggtctg accgagttcc
    10441 tcccggtctc ctcgagcgcg cacctgatcc tcgcgcagag gctcctgcct ggtttctcgc
    10501 agccggggat cctcttcgac gtgatgctcc acgtcgggac gctcgtggcc gtcgtcgtct
    10561 acttccggga gcggatcggg agcctcctcg cgggagccgt ctcgcgcgac ccggccgtgc
    10621 gccgcccggc ctggaagatc gcgatgctcc tcgcgacctc cgtcgcgctg acgggcgtcg
    10681 tcacgctccc cctcaagaag gtcgccctcg aagggatgga gagcctcccg aagatcgggc
    10741 tcgcgctcct cgcgacgtcg gcgctcctcg cggcagccca ggcaatcggc cggcggcgcg
    10801 gcgagggcgg ccggacgctc gaggagatgc gtttcttcga cgcggcgctc atcggcgcgt
    10861 tccagtccct ctcggccctc ttccggggtc tctcccgttc gggaaacacg atcgcgatgg
    10921 ggctcttcac gggcctctcg cgacgggccg ccgcggagta ctcgttcctc ctctccgtcc
    10981 cgacgatcct ctcggcggcg ctcgtcgaga acgtcagcga gtaccgcgcc tccggccacc
    11041 tcgtcacgga gggaagccac ctcggcgtct acctcgcggg gatggccgcc gccgccgtcg
    11101 tcggctacgc cgccgtcgcc ctcctcctcc gcctcgtcgt cgcgatgaag ctccaggcct
    11161 tcgtcctcta ctgcgcgctg ctcggcgccg tggtcctcgc cgcgggcctc tcctgatgcc
    11221 gatgaccgaa gtcccccgga gcccggagac gccgcatcgc cgccgggacg agttcctcgg
    11281 gatcctcctc gtcgcggccg gcctcctcct ggtggcgtcg ctcttctcgt acgacccggc
    11341 cgacccgtcg ctcttctccg ccgtcgccga cccggacgcg cggccgggca actgggcggg
    11401 ccgcgtcggc gcttcgttcg cggacggcgc gctccagttc ttcggcttcg cggccttcgc
    11461 cctgcccctc gtcgtcctcg cgctcggcat ggcgcggttc cgctcgcgcg ccgtcggcgc
    11521 gtggggaacg aaagcgctcg gcgtagttgt cgtcctcctc tcgtccgcgc ccctctgtca
    11581 cctcgccttc ggacgccctc gcctgctcgg aggcgggctc ggggcgggcg gcttcgtcgg
    11641 cgatctcgtc gccggagcgc tcgtcgccgc cttcaatccg cccggagccg cgattgccct
    11701 ctcggcggcc ctcctgatcg gcctcctcct cgtcgcctcg ctctcgctcg gggaggcggc
    11761 ccggcgcgtc gcgctccgga cctccgcgag cctcgccgct tggcggctcg agagggagag
    11821 gcgccgccag gtggaggaga aggagcggac gcgccgggcc gtcgtgaaga agcacctcga
    11881 gagggctctc gaggagcctc cgcccgcgga cgaggaggat gaggcgggcg gggaggtcgt
    11941 cgcgatcccc tccctcgcaa cgctccgcgt ccgcgagcgg agcggcgccg ggcagttctc
    12001 cgtccgccgg acggcgggtc ccccggtcgc cgcgggcgcc gagaccggcg tcgccgcgtc
    12061 gtgtctcccc gtccccgcgt cgcccggccg gcccgccccg cccggccgcc cctcgccggt
    12121 cgcgcgcccg gcccagctga agaagaccga gcccgccccg cagcgcgtcc tcccgttccc
    12181 gtcgccgaag gagtcgaacg ggtgggtctt cccccccgag aagctcctgc gccccgcccc
    12241 gaagcgggac cgggacaaga cgcgcgagga gttccagcag acgatggagc tcatcgccgc
    12301 caagtgcctc gagttcggcg tcgagggagc cgtcgacagc tatcgccccg gccccgtcgt
    12361 cacgacctac gagttcaagc cgtcggccgg cgtgaaggtc gcgcaggtcg ccggcctcga
    12421 gaacgacctc gccctcgcgc tcgcggccga gaaggtgagg atcgagcgga tcccggggcg
    12481 cgccgcgatc ggcatcgagg tcccgaaccg gaaccgcgac ctgatctccc tccgcgagat
    12541 cgtcgagagc gagcggttca agcgctcgcc gtccctcctg acgatcgccc tcgggatcga
    12601 cgtcgagggg aaccccgtcg tcgccgacct cgcgcggatg ccgcacctcc tcgtcgccgg
    12661 gatgaccggc gcgggcaaga gcgtcggcgt gaacgggatg atcacctcga tcctctacaa
    12721 ggcgcgtccc gacgaggtga agttcatctt cgtcgacccg aagatgaacg acatgaagga
    12781 ctacgacgac ctgccgcacc tcctcgtgcc ggtcgtcgtc gacccgaaga aggccgcgaa
    12841 cgcgctcaag tgggccgtcg acgagatgga ggggcgctac aagctcctct ccgagtggcc
    12901 cggcgtccgc aacatcgagc agtacaacgc cgcgatccgc gaccccgagg ccgtcgcgga
    12961 ggtccgcgag aagctcggcg agaagctgaa gaccgacggg cccgacggga cgatctcgcc
    13021 gatgccctac atcgtcgtcg tcatcgacga gctcgccgac ctgatgatga cggccccgaa
    13081 ggacatcgag gagtcggtcg c
//
LOCUS       DAOTEZ010000069        13077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3316_length_13077_cov_6.392566, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000069 DAOTEZ010000000
VERSION     DAOTEZ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13077
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3316_length_13077_cov_6.392566"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="P5164_05440"
     CDS             complement(<1..292)
                     /locus_tag="P5164_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRY43373.1"
                     /translation="MIDITLTPATFDVETVRADFPILSRKVRGRPLVYLDSANTTQKP
                     EAVIRASDRFYRETNANIHRSTYRLSEEATTAYEGAREKVRRFLNAASTREVV"
     gene            complement(289..1683)
                     /gene="sufD"
                     /locus_tag="P5164_05445"
     CDS             complement(289..1683)
                     /gene="sufD"
                     /locus_tag="P5164_05445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HRY43374.1"
                     /translation="MSATAAAPPAAEALAAATASALGAARALDARDASDLPHFVRNLR
                     RDGLEALGKLGLPRPSDEEWRYTSVATLARRLPDLLAAGAARGASPTVSAAAREALAC
                     FGPLAGEKRGLHALVLVNGRLDPALSHVDGLPAGVRFEPLSRAFAAPPPLLEQRLGLA
                     GAHRSHPFAALNAAFLSEGVWVEVPDGVVLPEPLVVLHVADASAGPLACHPRVVLTVG
                     ENAQASVVEAYCGVGEALVNAVTELYLLAHARLDHSVLQEEALDAFHVGTAEAHLGPG
                     AQLYSRVLSVGGRIARNELTVRLHGDGAGATLDGLFLARDAQQVDNHTLVDHARPHGG
                     SQQRYKGILSGEARGAFDGKVVVRPAGAFTDAHQSNRNLLLSDTARVDAKPQLEIYNN
                     DVKCTHGSATGRLDADALFYLRSRGFSEDEARSVLTLAFASEIVDRVPLEPLRAHLTE
                     RVLGWLSADAPGAR"
     gene            complement(1680..2468)
                     /gene="sufC"
                     /locus_tag="P5164_05450"
     CDS             complement(1680..2468)
                     /gene="sufC"
                     /locus_tag="P5164_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009849723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HRY43375.1"
                     /translation="MTTNLLEIRNLKARIAEDGTEILRGVNLTVKPGEVHAIMGPNGS
                     GKSTLAHVLAGRDGYEVTDGSVLYKGKDLLSLAPEERAREGVFLSFQYPVEIPGVANT
                     YFLRMALNAQRKHRGLPEIDAMDFLTLVEEKARLVEMEEALLSRNVNEGFSGGEKKRN
                     EIFQMAVLDPSLAILDETDSGLDIDALRVVASGVNSLRSPERSMLVITHYQRLLNYIV
                     PDVIHVLSEGRIVRTGDKSLALLLEEKGYAWLEEELPVGSGTAS"
     gene            complement(2471..3916)
                     /gene="sufB"
                     /locus_tag="P5164_05455"
     CDS             complement(2471..3916)
                     /gene="sufB"
                     /locus_tag="P5164_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HRY43376.1"
                     /translation="MSSNEVLSELTSQEYRHGFVTEIESDVFPPGLSEEIVAAISAKK
                     KEPQWLLDWRLKAFRHWQTMTEPRWAKVSYPAIDYQAIRYYAAPKSTKDGPKSLDEVD
                     PELLRTYEKLGVPLEERAILAGVAVDAVFDSVSVATTFRESLAKVGVIFCSFSEAVQE
                     HPQLVKKYLGTVVPYTDNFFATLNSAVFSDGSFCFVPKGVRCPMELSTYFRINQPDTG
                     QFERTLIVAEEGASVSYLEGCTAPKRDTNQLHAAVVELVALDDAKIKYSTVQNWYPGD
                     KDGKGGIYNFVTKRGKCAGRRSKISWTQVETGSSITWKYPSCVLLGDDSVGEFYSVAL
                     ANNFQQADTGTKMIHVGKNTRSTIVSKGISAGHGQNTYRGGVSIAKSAVGARNYSQCD
                     SLLLGSQCGAHTFPYLDVKTSTAMIEHEASTSKIGEDQLFYCRQRGLTSEDAVSLIVN
                     GFAKRVIKELPMEFAVEATKLLGVSLEGSVG"
     gene            complement(3913..4383)
                     /locus_tag="P5164_05460"
     CDS             complement(3913..4383)
                     /locus_tag="P5164_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HRY43377.1"
                     /translation="MIRMNKLTDYGVVLLTTFAHEPALGISARELSVRTGIPQPTVVK
                     LLKTLLKAGLLVSQRGTKGGYALSRAPEEIPVAVIIEALEGPLAITECSLPGTCAHER
                     GCPARPNWKAVNDVIQDALSRLTLADMTRPASPGATRTGSPLPAVDSAPRSIAS"
     gene            complement(4561..5946)
                     /locus_tag="P5164_05465"
     CDS             complement(4561..5946)
                     /locus_tag="P5164_05465"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HRY43378.1"
                     /translation="MTPLLADLACWWLQAGLLLGAALVIPAAVRLRDPATRLRVAQAA
                     LIAALALPLVQPAPRGGGTGDSNVRTAFSLVVPDERAAAPGAPSLETVVLAALAAGAF
                     LRLGWLGAGLLRLRALRRSARPLSPVPASVVSAAARVGATAELLVSSEVASPLAIGWR
                     RPAVILPERFDALEAAEQEAVACHELLHVKRRDSRAVLLEELARALFWSQPAAWKLLS
                     GISLAREQAIDGDVVRSTGDRRSYLRALARLAQFAQEAPSGALPFHSRSHLLRRVAHL
                     AKEVPMSRRNLALATTAAAVLLLLVGAAGAAAVPFGRSEGDSGAEPAASSAASPSDAV
                     REGVSGGVPGGVAGGVAGGVRGGVAEGADDGDVLKVGGDVTEPVVVSNVPPTYPEEAR
                     KNRIQGRVIVEAVISEKGIVTKVRAVESPDPMLTESALEAVKKWTYRPATKKGKPVKV
                     FLTATVSFKLS"
     gene            complement(5943..6320)
                     /locus_tag="P5164_05470"
     CDS             complement(5943..6320)
                     /locus_tag="P5164_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HRY43379.1"
                     /translation="MRPVPTPLTEAELEIMHVVWERGEATVRQVHDVLASRRPVAYTT
                     VMTVLGLLAKKGHLKREESGRAFVYRPAHPKGRVVSRMLDDFVERVFRGSAGPLVLAL
                     LKDRKLSKRELDEIVRLAEQEDR"
     gene            6448..7284
                     /locus_tag="P5164_05475"
     CDS             6448..7284
                     /locus_tag="P5164_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY43380.1"
                     /translation="MNVALEVSGLRKAYGAVEAVAAVSFRVAGGTIVGLLGPNGAGKT
                     TTVSIVCGLVPPDAGEVKVGGVALAGDTDPAKGRLGLVPQEPALYEELTAEDNLRFFG
                     ALQGMEGDRLASRIDDVLRLTGLLDRRRDRVKVYSGGMKRRLNLAAGLLHDPEVLLLD
                     EPTVGVDPQSRNAIFESLEALRDAGKAILYTTHYMEEAERLCDRIVIVDRGRVVAEGT
                     RSELGALLPGNGARDLDDEARAALALLAARGIAVPGAGGGTPRLEEVFLHLTGHALRD
                     ER"
     gene            7281..8579
                     /locus_tag="P5164_05480"
     CDS             7281..8579
                     /locus_tag="P5164_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY43381.1"
                     /translation="MRQVSAIVVHELKLFFSDRRSVVVGIVTPILLATFFGFLFSGAG
                     RQDAAKIPVLVADADGSAVSRAIVAGLAADALLSVSPATAEDARRAVRSGKVAAGVVL
                     PKGFGVGAVQAFFGAAAAPEIVLLVDPSRATEAGLVRGLLAEKVFRESSREAFGGATG
                     TTVLQESLGALEADPSPPTDERQALKDLLRDVTRWQGAAKGGTAGGGTAGGPGTAVPF
                     TLKEEPVTARPGVTYNSYAHSFAGMGIQFLLFVALDFGIAFLLERRGGIWKRLRAAPL
                     SRSSLLLGKAVAGALVGLVSVGATFAFGIVVLGVRVEGSVSGFVLLLLASALMASSFG
                     LLLAALGRTPGATRGLAILAVLLLVMLGGGWVPAFVFPAWLQKVTLVLPTRWAIDGLD
                     AVTWRGAGLVAGALPAVAVLLGFTVLFLLVARLRFRWTDG"
     gene            complement(8857..9606)
                     /locus_tag="P5164_05485"
     CDS             complement(8857..9606)
                     /locus_tag="P5164_05485"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRY43382.1"
                     /translation="MLQPATPPDRPRPALLASIGVHVTAFSVLGFGPLLLFPDVPGWR
                     GEVWAVTQPDLSVLREKRTVDLRDRPAPVRSGGALRGGGVPAAARPGTADQPLRTTVQ
                     PGSVPDELPAETPYEAGGGAGVPGGVDDGASGSPGDGAGPGTGGGDAPYDARGGALPP
                     DLVLPVPLETPAPPYSEAARLTRASGVVVLSATIAADGRVVDVVVENDAHPLLERLAV
                     EAISRWRYAPARVGTHPVSVVLRVTLTFRLR"
     gene            9725..10120
                     /locus_tag="P5164_05490"
     CDS             9725..10120
                     /locus_tag="P5164_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014799690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HRY43383.1"
                     /translation="MELSLTTPALLFPAVSLLLLAYTNRFLALASLVRSLHTRYREQP
                     DALILRQIDNLRYRIHLIQAMQATGVSSLLVCVVCMFVLFFGKVVLGKFLFAGSLVLM
                     IASLGLSVREIQVSVKALDLHLHDLEERP"
     gene            10135..11286
                     /locus_tag="P5164_05495"
     CDS             10135..11286
                     /locus_tag="P5164_05495"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY43384.1"
                     /translation="MTRPFVLVVLSTAVLFFGFYFLIPVLPPYAAARGASKGEVGLVV
                     GLASMTALATRAAFARALDRKGRRPFALGGLALFAAATALLPLAGSLVPLLLVRGVQG
                     IGWGLATTAIASLVADLAPAERRGEAIGVWGLAPTVAMALGPAAGGTLSRLGGPATAF
                     LGTALLFLLALGLLLPVAEPRHAAGQARTASLPAGALLPCAVLFLSSLSYGAILAFVP
                     VELGPRRTGAYFLLYAVSILVVRPFAGRLSDRHGRVAVAVPGLLLGAAGVVGTGLARG
                     PLSLAAAAVLYGAGMAGLSFPALVAWTVDLAGEARGTAMAAFYGAYDLAIALGAFALG
                     ALYERHGFAALNLAGAAGVVAASLLALAFSGAAGRRRPDIGNPEPPRAR"
     gene            11295..11672
                     /locus_tag="P5164_05500"
     CDS             11295..11672
                     /locus_tag="P5164_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43385.1"
                     /translation="MNVSRLALVVAALLVLTGIGGYAAFQSPTALIPVGFGVLLGACG
                     LVARKESLRKHAMHAAAAVALLGFVPSAPGLLGLPALLAGEAQRPAAVVLRSVMALLL
                     LGFLVAAVRSFVAARRARPAGES"
     gene            11720..12223
                     /locus_tag="P5164_05505"
     CDS             11720..12223
                     /locus_tag="P5164_05505"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRY43386.1"
                     /translation="MRLASATLDSPVGRLLAVVWDGRLCTLTFDADAASTREHLLARF
                     GTDLFPAHRDPAGVCMRLRAYFRGELEALDDVEVDLAGTPFQEKVWAELRRIRPGSTR
                     SYADVASAVGEPKALRAVGMAAARNPAVLVVPCHRVVASDGSLHGFSGGIERKRWLLV
                     HEGALLA"
     gene            12290..12760
                     /locus_tag="P5164_05510"
     CDS             12290..12760
                     /locus_tag="P5164_05510"
                     /inference="COORDINATES: protein motif:HMM:NF027592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HRY43387.1"
                     /translation="MKTTLRTTLAAALLAALAATPAAAATKYGKGVSEATPVRLSELM
                     AKPDAYVGKVVKVEGLITEVCAKRGCWIDVAGDVEFQKIRIKVEDGVIVFPMTDKGKK
                     VVAEGTFKKTELTKEQAIAKAKHECEEKGTKFDPASVTGPATVYQIQGTGAVVD"
     gene            12771..>13077
                     /locus_tag="P5164_05515"
     CDS             12771..>13077
                     /locus_tag="P5164_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43388.1"
                     /translation="MNWRKLNNVLHRDLGYLAAGLTLVYAISGVALNHRHQWNPSWKL
                     VVEERTFAPVPVSDRETMAAALVERLGLPGPPKSSFRSAPHLVELFYEGWSVKADATA
                     "
BASE COUNT         1712 a         4588 c         4847 g         1930 t
ORIGIN      
        1 agacgacctc gcgcgtggag gcggcgttca ggaaccggcg gaccttctcc cgcgcccctt
       61 cgtaggcggt cgtggcctcc tccgagaggc gataggtgga gcggtggatg ttcgcgttcg
      121 tctcgcggta gaagcggtcc gacgcgcgga tgaccgcctc gggcttctgc gtcgtgttcg
      181 cgctgtcgag gtagacgagg ggccggccgc ggaccttccg cgagaggatc gggaagtcgg
      241 cccggaccgt ctccacgtcg aaggtggccg gcgtcagggt gatgtcgatc atcgtgcccc
      301 cggcgcgtcc gcggagagcc agcccaggac gcgctcggtc aggtgcgcac ggagcggctc
      361 gagcgggacg cggtcgacga tctcgctcgc gaacgcgagc gtcaggacgc tccgggcctc
      421 gtcctcggag aagccccggg agcgcaggta gaagagggcg tccgcgtcga ggcggccggt
      481 cgcggagccg tgggtgcact tgacgtcgtt gttgtagatc tcgagctggg gcttggcgtc
      541 gacgcgggcc gtgtcggaaa gaaggaggtt ccggttcgac tggtgggcgt cggtgaacgc
      601 cccggccggg cggacgacga ccttcccgtc gaacgccccg cgcgcctccc cggagaggat
      661 ccccttgtag cgctgctggc tcccgccgtg cggccttgcg tggtcgacga gcgtgtggtt
      721 gtcgacctgc tgcgcgtcgc gcgcgaggaa gagcccgtcg agggtcgccc cggccccgtc
      781 gccgtggagg cggacggtca gctcgttccg ggcgatccgc ccgccgaccg acagcacacg
      841 cgagtagagc tgggccccgg gcccgagatg ggcctcggcc gtgccgacgt ggaacgcgtc
      901 gagcgcctcc tcctggagga cggagtggtc gaggcgggcg tgagcgagga ggtagagctc
      961 ggtgacggcg ttcacgagcg cctctccgac cccgcagtag gcctcgacga cggaggcctg
     1021 ggcgttctcg ccgacggtga ggacgacgcg cgggtggcag gcgagaggcc ccgcgctcgc
     1081 atcggcgacg tggaggacga cgagcggctc ggggaggacg acgccgtccg ggacctccac
     1141 ccagacgccc tcggagagga aagccgcgtt cagggcggcg aacgggtggc tccggtgggc
     1201 gccggcgaga cccaggcgct gctcgaggag cggcggcgga gcggcgaagg cgcgggagag
     1261 gggctcgaag cggacgcccg ccgggagacc gtcgacgtgc gaaagggccg ggtcgagccg
     1321 cccgttcacg aggacgaggg cgtggaggcc tctcttctcg ccggcgagag ggccgaagca
     1381 ggcgagcgcc tctcgggccg cggcggagac cgtcggcgaa gcgccgcgcg ccgcgccggc
     1441 cgcgaggagg tccggaaggc gccgggcgag cgtcgcgacg ctggtgtatc gccattcctc
     1501 gtcggaaggg cgagggagcc cgagcttccc gagcgcctcg aggccgtcgc ggcggaggtt
     1561 ccggacgaag tgggggagat cggacgcgtc gcgggcgtcg agcgctcggg ccgcgcccag
     1621 ggccgaggcc gtggccgcgg cgagcgcctc ggcggcgggc ggggcggcgg ccgtcgcgct
     1681 caagaagccg tcccgctccc gacggggagc tcctcctcga gccacgcgta gcccttctcc
     1741 tcgaggagga gggcgagcga cttgtcgccc gtgcggacga tccggccttc ggagaggacg
     1801 tggatgacgt cggggacgat gtagttcagg agccgctggt agtgcgtgat gacgagcatc
     1861 gagcgctccg gcgaccggag ggagttcacg ccggaggcga cgacgcggag ggcgtcgatg
     1921 tcgaggccgg agtcggtctc gtcgaggatc gcgagagacg ggtcgaggac cgccatctgg
     1981 aagatctcgt tcctcttctt ctcgccgccc gagaagccct cgttcacgtt gcgcgagagg
     2041 agcgcctcct ccatctcgac gaggcgggcc ttctcctcga cgagcgtcag gaagtccatc
     2101 gcgtcgatct cggggaggcc gcggtgcttc cgctgcgcgt tcagggccat ccggaggaag
     2161 tacgtgttcg ccacgcccgg gatctcgacc ggatactgga acgagaggaa gaccccttcg
     2221 cgggcgcgct cctcgggggc gagggagagg aggtccttcc ccttgtagag gacggagccg
     2281 tcggtcacct cgtagccgtc ccggccggcg aggacgtggg cgagggtgct cttgcccgag
     2341 ccgttggggc ccatgatcgc gtggacctcg cccggcttca cggtgaggtt cacgccgcgg
     2401 aggatctcgg tgccgtcctc ggcgatgcgg gccttgaggt tgcggatctc gaggaggttc
     2461 gtcgtcacgt tcagcccacg cttccttcga ggctcacgcc gaggagcttc gtcgcctcga
     2521 cggcgaactc catcgggagc tccttgatga ctctcttggc gaagccgttg acgatgagcg
     2581 acacggcgtc ctcgctcgtc aggccccgct ggcggcagta gaagagctgg tcctccccga
     2641 tcttcgacgt cgacgcctcg tgctcgatca tcgcggtcga ggtcttcacg tcgaggtagg
     2701 ggaaggtgtg cgccccgcac tgggacccga ggaggagcga gtcgcactgg gagtagttgc
     2761 gggcgccgac cgccgacttc gcgatcgaca cgcccccgcg gtatgtgttc tggccgtggc
     2821 cggcggagat ccccttcgag acgatcgtcg agcgggtgtt cttcccgacg tggatcatct
     2881 tcgttccggt gtcggcctgc tggaagttgt tcgcgagggc gaccgagtag aactcgccga
     2941 ccgagtcgtc gccgaggagg acgcagctcg ggtacttcca ggtgatcgac gagccggtct
     3001 ccacctgcgt ccaggagatc ttcgagcggc ggccggcgca cttgccgcgc ttcgtgacga
     3061 agttgtagat gccccccttg ccgtccttgt cgccggggta ccagttctgg acggtggagt
     3121 acttgatctt cgcgtcgtcc agcgcgacga gctcgacgac ggcggcgtgg agctggttcg
     3181 tgtcgcgctt cggggcggtg cacccctcga ggtaggagac ggaagcccct tcctcggcga
     3241 cgatgagcgt ccgctcgaac tgccccgtgt cgggctggtt gatccggaag taggtcgaga
     3301 gctccatcgg gcagcggacc cccttgggca cgaagcagaa ggagccgtcg gagaagaccg
     3361 ccgagttgag cgtcgcgaag aagttgtcgg tgtacggcac gaccgttccc aggtacttct
     3421 tcacgagctg cgggtgctcc tgaaccgcct cggagaagga gcagaagatc acgccgacct
     3481 tcgcgaggct ctcgcggaac gtcgtcgcga ccgacacgct gtcgaagacg gcgtcgacgg
     3541 cgacgccggc gaggatcgcc cgctcctcga gcggcacgcc cagcttctcg taggtgcgga
     3601 gcagctcggg gtcgacctcg tcgaggctct tcggcccgtc cttcgtcgac ttcggcgcgg
     3661 cgtagtagcg gatcgcctgg tagtcgatcg cggggtagga gactttcgcc cacctcggct
     3721 cggtcatcgt ctgccagtgg cggaaggcct tgagccgcca gtcgaggagc cactgcggct
     3781 ccttcttctt cgcggagatg gccgccacga tctcctcgga caggccggga gggaagacgt
     3841 cggattcgat ctccgtgacg aagccgtgcc ggtattcctg gctcgtcagc tcggagagga
     3901 cttcgttcga gctcatgaag cgatgctcct gggggcggaa tcgaccgccg gaagcgggga
     3961 cccggtgcgg gtcgcgcccg gcgacgccgg gcgcgtcatg tcggcgaggg tcaggcgcga
     4021 gagcgcgtcc tggatgacgt cgttcacggc cttccagttc ggacgggcgg ggcagccccg
     4081 ctcgtgcgcg caggtcccgg gaaggctgca ctccgtgatc gcgagaggcc cttcgagcgc
     4141 ctcgatgatc acggcgaccg gaatctcctc cggcgctctc gaaagcgcgt agcccccctt
     4201 cgtcccgcgc tgggagacga ggaggcccgc cttcagcagc gtcttcagca gcttcacgac
     4261 ggtcggctgc gggatgcccg tccggacgga cagctcccgc gcgctgatcc cgagcgccgg
     4321 ctcgtgcgcg aacgtcgtca ggaggacgac cccgtaatcg gtgagcttgt tcatccggat
     4381 catgtcgggt gcttcctgcc gccgccccaa tcgagacgca tgctcaatta ggacctctcc
     4441 agtactgtat ttccggaacc cgggggtgtc aagcgcggca gagccgagaa gacggggcgg
     4501 aaagcagagt gccccggctc ctttcggcgc cggggcacga gatccggagc gagggctccg
     4561 tcaggacagc ttgaacgaga cggtggccgt gaggaagacc ttcacgggct tccccttctt
     4621 cgtggccggc ctgtaggtcc acttcttcac ggcttcgagc gccgattccg tcaacatcgg
     4681 atcgggcgat tcgaccgccc tcaccttcgt gacgatcccc ttctcgctga tgacggcttc
     4741 gacgatcacg cggccctgga tccggttctt ccgcgcctcc tcggggtagg tcggcgggac
     4801 gttgctgacg acgaccggct ccgtcacgtc gcccccgacc ttcagcacgt cgccgtcgtc
     4861 ggctccctcc gcgacgccgc cgcggacgcc tccggcgacc cccccggcca cccctcccgg
     4921 gacgcccccg gaaacgccct cccggacggc gtccgacggc gaggcggcgg acgaggcggc
     4981 gggctccgcc ccggaatcgc cctcggagcg tccgaacggg acggcggcgg cccccgcggc
     5041 gccgacgagg aggaggagga ccgccgcggc ggtcgtggcg agggcgaggt tcctgcgtga
     5101 catggggacc tcctttgcga gatgggccac ccttcgaaga aggtggctcc gcgagtggaa
     5161 ggggagagcc cccgacggag cctcctgcgc gaactgcgcg aggcgggcga gcgcgcgaag
     5221 gtaggagcga cgatcgccgg tggaacggac gacgtccccg tcgatcgcct gctcgcgcgc
     5281 gagcgagatg cccgagagga gtttccaggc ggccggctga gaccagaaga gcgcccgcgc
     5341 gagctcctcg aggaggacgg cccgggagtc gcggcgtttc acgtgcagga gctcgtggca
     5401 ggcgacggcc tcctgctcgg cggcctcgag agcgtcgaag cgctccggga ggatcacggc
     5461 cgggcggcgc cagccgatcg cgaggggcga ggcgacctcg gacgacacga ggagctcggc
     5521 ggtcgcgccg acgcgcgccg ccgcggagac gaccgaagcc gggaccgggg agagcgggcg
     5581 cgcgctgcgg cgaagcgccc ggaggcgcag gagccccgct ccgagccagc cgaggcgaag
     5641 gaacgcgccc gcggcgagcg cggcgaggac gaccgtctcg agggagggag cgcccggcgc
     5701 cgcggcgcgc tcgtcgggga cgacgaggga gaacgccgtg cggacgttgg agtctcccgt
     5761 cccgcccccg cggggcgccg gctggacgag ggggagggcg agggccgcga tgagcgccgc
     5821 ctgcgcgacc cgcagccgtg tcgccgggtc gcgcagccgg accgccgcgg ggatgacgag
     5881 cgcggcgccg aggagaaggc ccgcctgcag ccaccagcag gcgaggtcgg cgaggagcgg
     5941 cgtcatcggt cctcctgctc cgcgagccgc acgatctcgt cgagctcgcg cttcgacagc
     6001 ttccggtcct tcaggagcgc gaggacgagc ggccctgcgg agccgcggaa gacccgctcc
     6061 acgaagtcgt ccagcatccg cgagacgacg cgccccttcg ggtgggcggg ccggtagacg
     6121 aaggcccgcc ccgactcctc ccgcttcagg tgacccttct tggcgaggag gccgagcacc
     6181 gtcatgacgg tcgtgtaggc gacggggcgc cgcgacgcga ggacgtcgtg gacctgccgc
     6241 accgtcgcct ccccgcgctc ccagacgacg tgcatgatct ccagctcggc ttccgtgagg
     6301 ggcgtcggta cgggtcgcat agtactaaaa acttagtact acgcgcttcg gatgtcaagg
     6361 gctgctcttc cgagcctggc ccgagggaaa cccggcgggt ccgctcccgt atcctgaagg
     6421 catggccgtc gccacgaggt ccccgtcctg aacgtcgccc tcgaggtctc cggtctccgg
     6481 aaggcgtacg gcgccgtcga ggccgtcgca gccgtttcgt tccgcgtcgc cggagggacg
     6541 atcgtcggcc tcctcgggcc gaacggggcc ggcaagacga cgaccgtctc gatcgtctgc
     6601 ggtctcgtcc ctcccgacgc gggagaggtg aaggtcgggg gcgtcgccct tgcgggggac
     6661 acggacccgg cgaaggggcg tctcggcctc gtgccgcagg agccggcgct ctacgaggag
     6721 ctgacggccg aggacaacct ccggttcttc ggggcgctcc aggggatgga gggggaccgc
     6781 ctcgcctcgc ggatcgacga cgtcctgcgg ctcaccgggc tcctcgaccg ccgccgggac
     6841 cgcgtgaagg tctacagcgg cggaatgaag cggcgcctga acctcgcggc cgggctgctc
     6901 cacgaccccg aggtcctcct cctcgacgag ccgacggtcg gcgtcgatcc ccagagccgg
     6961 aacgcgatct tcgagagcct cgaggcgctc cgggacgccg ggaaggcgat cctctacacg
     7021 acgcactaca tggaggaggc cgaacggctc tgcgaccgca tcgtcatcgt cgaccgcggc
     7081 cgggtcgtgg ccgaggggac gaggagcgag ctcggcgcgc tcctgccggg caacggcgct
     7141 cgcgatctcg acgacgaggc tcgcgccgcc ctcgccctcc tcgccgcgcg ggggatcgcc
     7201 gttcccggag cgggcggcgg gacgccgcgg ctcgaggagg tctttctcca cctgacgggc
     7261 cacgccctga gggacgagcg gtgagacagg tctccgcgat cgtcgtccac gagctgaagc
     7321 tcttcttctc ggaccggcga tccgtcgtcg tcgggatcgt gacgccgatc ctcctggcga
     7381 cgttcttcgg cttcctcttc tcgggcgcgg ggcggcagga cgcggcgaag atcccggtcc
     7441 tcgtcgccga cgcggacggc agcgccgtgt cgcgcgcgat cgtggcgggc ctcgcggccg
     7501 acgctctcct ctccgtgagc ccggcgaccg cggaggatgc gcggcgggcc gtgcgaagcg
     7561 ggaaggtcgc cgcgggggtc gtcctgccga agggcttcgg cgtgggcgcc gtgcaggcgt
     7621 tcttcggcgc cgcggcggcg cccgagatcg tcctcctcgt cgacccctcg cgcgcgacgg
     7681 aagcgggcct cgtgcgggga ctcctcgccg agaaggtctt ccgcgaatcg tcgcgggagg
     7741 ctttcggcgg agcgacgggg acgacggtcc tccaggagag cctgggcgcg ctcgaggccg
     7801 atccgtcgcc tccgacggac gagcgtcagg cgttgaagga cctgctgcgc gacgtgacgc
     7861 gctggcaggg ggcggcgaag ggcggaacgg ccgggggcgg cacggcgggc gggccgggaa
     7921 cggcggtccc cttcacgctg aaggaagagc cggtcaccgc gcgcccgggg gtgacgtaca
     7981 acagctacgc acactccttc gccgggatgg ggatccagtt cctcctcttc gtcgctctcg
     8041 acttcggcat cgccttccta ttggagcgtc gaggcgggat ctggaaacgg ctccgggccg
     8101 ccccgctctc ccgctcgtcg ctcctcctcg ggaaggcggt cgccggcgcg ctcgtgggcc
     8161 tcgtctcggt gggggcgacg ttcgccttcg gcattgtggt cctcggggtc cgggtcgagg
     8221 ggagcgtttc gggcttcgtc ctcctccttc tcgcgtcggc gctgatggcc tcctcgttcg
     8281 gcctcctcct cgcggccctg ggaaggacgc ccggggccac gcgcggcctc gcgatcctcg
     8341 ccgtcctcct cctcgtcatg ctcggcggcg gctgggtccc ggccttcgtc tttccggcgt
     8401 ggctgcagaa ggtgacgctc gtcctgccga cgcgctgggc gatcgacggc ctcgatgcgg
     8461 tgacgtggcg cggcgccggc ctcgtggccg gcgcccttcc ggccgtcgcc gtcctcctcg
     8521 gcttcacggt cctgttcctc ctcgtcgcgc gcctgcgctt ccgctggacc gacgggtaac
     8581 ccgcacgttc cggcgccttc cggaccgggt cgccccgggg aggcccccgc atcgggcgcc
     8641 ccgcatcggt gccaaccccg aatcggtgcc cccccccgaa tcggtgccag gcgtgaaatc
     8701 gtgtattgtc tctgacaata cacgatttca cgcctggcac caaacacagg cgccagaccc
     8761 ggggaaccgg accgccggag tctgggatcg tggccgacag ggcggatccc tcctcttcgc
     8821 ccgccggcgg cgcgatctcc tcgggtgtgc gggtcctcag cggagacgga acgtgagcgt
     8881 gacgcggagg acgacggaga ccgggtgggt gccgacgcgc gccggggcgt agcgccagcg
     8941 cgagatggcc tcgacggcga gccgctcgag gagggggtgc gcgtcgttct cgacgacgac
     9001 gtcgacgacc cggccgtcgg cggcgatcgt cgccgagagg acgacgacgc ccgaggcgcg
     9061 ggtgagccgg gccgcctcgc tgtacggcgg ggccggggtc tccagcggca cggggaggac
     9121 gaggtctggc gggagcgctc cgccgcgcgc gtcgtagggc gcatcgccgc cacccgtccc
     9181 gggaccggcg ccgtcccccg gcgaacccga ggccccgtcg tcgacgccgc cggggactcc
     9241 tgccccgccg cccgcctcgt acggcgtctc ggcagggagc tcgtccggga cgctccccgg
     9301 ctgcaccgtc gtccggagag gttgatccgc ggtcccggga cgggccgctg cgggaacgcc
     9361 gcctccgcgg agcgccccgc cggagcggac gggcgccgga cggtcgcgca ggtcgacggt
     9421 ccgcttctcg cggagtaccg acaggtcggg ctgcgtcacg gcccagacct cgccgcgcca
     9481 gccggggacg tcggggaaga gaaggagcgg cccgaatccg aggaccgaga aggccgtcac
     9541 gtggactccg atcgacgcga gaagcgcggg acggggccgg tcggggggcg tcgcgggctg
     9601 gagcatcgtc gtcctcctcc gtcgtttccc ggtgagaccc ggagcagggc gcggagttcc
     9661 cggacccccg cgggcgcact gcgccgagcc cggcgccccg ggtctcggct atcgtccggg
     9721 cgccatggag ctctccctca cgacacccgc gctcctcttc cccgcggtct cgctcctcct
     9781 cctcgcctac acgaaccgct tcctcgcgct cgccagcctc gtgcggagcc tccacacgag
     9841 ataccgcgag cagcccgacg cgctcatcct gcggcagatc gacaacctga ggtacaggat
     9901 ccacctgatc caggcgatgc aggcgacggg cgtctcgagc ctcctcgtct gcgtcgtctg
     9961 catgttcgtc ctcttcttcg ggaaggtcgt cctcgggaag ttcctcttcg cgggcagcct
    10021 cgtgctgatg atcgcttcgc tcggcctctc ggtgagggag atccaggtct ccgtgaaggc
    10081 gctcgacctc cacctccacg acctcgagga gcgcccctga cggaccggcg gatcctgacc
    10141 cgtccgttcg tcctcgtcgt cctctcgacg gccgtcctct tcttcggctt ctacttcctc
    10201 atcccggtcc tcccgccgta cgcggccgcg cgcggcgcct cgaagggcga ggtcggcctc
    10261 gtcgtcggcc tcgcgtcgat gacggccctc gccacgcgcg ccgcgttcgc tcgcgccctc
    10321 gaccggaagg ggcggcgacc cttcgctctc ggcggactcg cgctcttcgc ggcggcgacg
    10381 gcgctcctcc cgctcgccgg gtcgctcgtg ccgctgctcc tcgtgcgggg cgtgcagggg
    10441 atcggctggg gcctcgcgac gacggcgatc gcgtcgctcg tcgcggacct cgccccggcc
    10501 gagcggcgcg gagaggcgat cggcgtctgg ggcctcgcgc cgacggtggc gatggcgctc
    10561 ggccccgcgg cgggcgggac gctgtcgcga ctcggaggac ccgcgaccgc gttcctcggg
    10621 acggcgctcc tcttcctgct ggccctcggc cttctcctgc ccgtcgcgga gccgcgccac
    10681 gcggcggggc aggcccgcac ggcgagtctc ccggccggcg cgctcctgcc gtgcgcggtg
    10741 ctcttcctgt cctctctgtc gtacggcgcc atcctggcct tcgtcccggt cgagctcggc
    10801 cccaggagga ccggggccta cttcctcctc tacgcggtgt cgatcctcgt ggtccggccg
    10861 ttcgcaggcc gcctctcgga ccggcacggg cgggtcgccg tcgcggtccc cggcctcctc
    10921 ctcggagccg ccggcgtcgt cgggacgggc ctcgcgcgcg ggccgctctc cctcgcggcc
    10981 gcggccgtcc tctacggcgc cgggatggcg ggcctctcct tcccggccct cgtcgcctgg
    11041 accgtcgacc tcgccgggga ggcgcgggga acggcgatgg cggcgttcta cggcgcgtac
    11101 gacctggcca tcgcgctcgg cgcgttcgcc ctcggcgcgc tctacgagag gcacggcttc
    11161 gcggcgctca acctggccgg cgcggcgggg gtggtcgccg cctcgctcct cgcgctcgcg
    11221 ttctccggag ccgcgggacg gcgccgtccg gatatcggga atccggagcc ccctcgcgcg
    11281 cgttagcgct tctgatgaac gtctcccgtc tcgccctcgt cgtcgccgcc ctcctcgtcc
    11341 tgaccggcat cggcggctac gccgcgttcc agagcccgac ggccctcatc ccggtcgggt
    11401 tcggagtcct cctcggagcc tgcgggctcg tcgcgcgcaa ggagagcctc cgcaagcacg
    11461 cgatgcacgc ggccgccgcg gtggcgctcc tcggcttcgt cccgtcggcc cccggcctgc
    11521 tcgggctgcc ggcgctcctg gccggcgagg cccagcggcc cgccgccgtc gtcctccgga
    11581 gcgtgatggc gctcctcctc ctcgggttcc tcgtcgccgc ggtccggtcg ttcgtggcgg
    11641 cgcggagggc gcgcccggcc ggggagagct gagacggccg ttcccgcgcg cggcggagaa
    11701 atggggtagc gttccgggcg tgaggctcgc gagcgcgacc ctcgactctc cggtcgggcg
    11761 gctcctcgcc gtcgtctggg acgggcggct ctgcacgctg acgttcgacg ccgacgcggc
    11821 ctcgacccgg gagcatctcc tcgcccgctt cgggaccgac ctcttcccgg cccaccgcga
    11881 ccccgccggc gtctgcatgc ggctcagggc ctacttccgg ggagagctcg aggcgctcga
    11941 cgacgtggag gtcgatctgg ccgggacgcc gttccaggag aaggtgtggg cggagctccg
    12001 ccgcatccgg ccggggtcga cgcgctccta cgccgacgtg gcgtccgccg tcggcgagcc
    12061 gaaggcgctg cgggcggtcg ggatggccgc ggcgcgcaac ccggcggtcc tcgtcgtccc
    12121 gtgccaccgg gtcgtcgcga gcgacgggag cctccacggc ttctcgggag ggatcgagcg
    12181 gaagcgctgg ctcctcgtcc acgagggggc tctcctcgcc tgaggcggag gcgtgcacgg
    12241 cgacggtaga atccgccgct cgaacgcccg agagcaggag acacgaccga tgaagacgac
    12301 cctccgcacg accctcgccg ccgccctcct cgcggcccta gccgccacgc ccgccgccgc
    12361 cgccacgaag tacggcaagg gggtctccga ggcgaccccc gtgaggctct ccgagctgat
    12421 ggcgaagccc gacgcgtacg tcgggaaggt cgtgaaggtg gaagggctca tcaccgaggt
    12481 ctgcgcgaag cgcggctgct ggatcgacgt cgccggcgac gtggagttcc agaagatccg
    12541 gatcaaggtc gaggacggcg tcatcgtctt tccgatgacc gacaagggga agaaggtcgt
    12601 cgccgagggg acgttcaaga agacggagct gacgaaggag caggcgatcg cgaaggcgaa
    12661 gcacgagtgc gaggagaagg ggacgaagtt cgacccggcc tccgtgaccg gcccggcgac
    12721 cgtctaccag atccagggga ccggcgccgt cgtcgactga gcgctccgcc ttgaactggc
    12781 ggaagctcaa caacgtcctc caccgcgacc tcggctacct cgcggcgggg ctgacgctcg
    12841 tctacgcgat ctcgggcgtg gcgctgaacc atcggcacca gtggaacccg agctggaagc
    12901 tcgtcgtcga ggagcggacg ttcgcccccg tgccggtctc ggaccgcgag acgatggcgg
    12961 cggcgctcgt cgagcgcctg gggctgcccg ggccgccgaa gtcgtcgttc cgctccgcgc
    13021 cccacctcgt cgagctcttc tacgaggggt ggagcgtgaa ggccgacgcg acggcgg
//
LOCUS       DAOTEZ010000070        13046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3328_length_13046_cov_6.067508, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000070 DAOTEZ010000000
VERSION     DAOTEZ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13046
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3328_length_13046_cov_6.067508"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="P5164_05520"
     CDS             complement(<1..900)
                     /locus_tag="P5164_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRY43389.1"
                     /translation="MKERRAVVTGLGLVTPLGTGLGATRRALAEGRCAVAPATLFDAR
                     PFASPLAGEVHDLDPRPYFRVPKALKLCDRRTRFAVCAAAMALEDAGFPAGPSEGLGV
                     SIGTSGSDLGAEELSAALARAEDPALPARDTDAFGEAALAGLNPLWLLVHLPNMTSAH
                     VAIQQEARGPNTTVMTGWAAGLAAIVEGAAWIAEGRADAVLAGGADSAVHPFAFAALE
                     QEGLLSGPSPLVPAEGAAVVLLEEQEAALARGARILGEVGAHALSAGTRLAALAAGAT
                     SAPSLPLERLAGNLLAAHAPAALV"
     gene            complement(897..1937)
                     /gene="lpxD"
                     /locus_tag="P5164_05525"
     CDS             complement(897..1937)
                     /gene="lpxD"
                     /locus_tag="P5164_05525"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRY43390.1"
                     /translation="MRKTTLGDIAEFLGGEIDGDRETPIDRLEPPEEAGEGALAVLAD
                     RKALAGMAACRASALVVPPGTSASGRSVIRVPHPRRAFVALLAYFAPDDARGPGLDPR
                     ASVDPGAEMGEGAWVGPGAVVEAGARLGARVRVHPLAVVGEGAKVGDDSVLFPGVVLY
                     PGVTVGRRVRIHAGSVIGSDGFGYERDAGGVQHKVPQIGTVEIADDVEIGSNSCVDRA
                     TVGATRIGAGTKIDNLVQIGHNTQVGEHCCVIGQAGLSGSVKVGRLSILAGQSGIADH
                     VTLAEGTVVGAQAGVHRDIGPGIWHGAPALPAAEARRVFPIVARLPELLRRIKELEEK
                     VAALEGRDGPGT"
     gene            complement(1934..3142)
                     /gene="lpxB"
                     /locus_tag="P5164_05530"
     CDS             complement(1934..3142)
                     /gene="lpxB"
                     /locus_tag="P5164_05530"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HRY43391.1"
                     /translation="MRILLSAGEVSGDVAGSRLVRALRARRSDLAFFGLGGPRMAGAG
                     VELLRSTNALGTVGISESFRVVPGLVRAFASLRRRVAGSPPDAAVLIANDVFHVVLGR
                     WLRARGVPTLSYFPPQVWIWRSLARVFAPSFDEILTSFPDEERVWRAAGASTTFVGHY
                     LGDVLRPATEEERRHARGALGLPAGPLVAVLPGSRELEVRRLTPVLLDAARLLHERDG
                     RIRFILPVADSAFAPRIEAEVARRGLEGVLGTTSSGHDALRAADLAILASGTASLEAA
                     LIGTPMVIAYRLAALTMGVVRSAISLGLIDSDTVGLPNLVLGRRAIPEHIQDRASGFA
                     LAAAAERLLGDESLLEKQRQALGEVSRLLFAGGSDARAAEAVLAAAERRPERGAAFGG
                     AIVREEGSGT"
     gene            complement(3139..3336)
                     /locus_tag="P5164_05535"
     CDS             complement(3139..3336)
                     /locus_tag="P5164_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43392.1"
                     /translation="MKRRLGLFLLLSGTFVAARVLVARAVDRPVPEARLLALHAVAVP
                     LAQLALLEAAGRLRARGRGIA"
     gene            complement(3333..3815)
                     /locus_tag="P5164_05540"
     CDS             complement(3333..3815)
                     /locus_tag="P5164_05540"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HRY43393.1"
                     /translation="MRFVLVDQFLSVEPGRGVTARKTFRPEDPVFADHFPGFPIVPGV
                     LLTESMGQAAGWAIATKVGPDRFPLLMMVERAKFRRLVRPGDEIRLEADLEETREDVW
                     TARTRASVGDEKVADARLVFHLVARPSDSAGFDGWRAGILRETGLGRLVETVLSSTAE
                     "
     gene            complement(3830..4546)
                     /locus_tag="P5164_05545"
     CDS             complement(3830..4546)
                     /locus_tag="P5164_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43394.1"
                     /translation="MNGDGVPQAGERMGYPQRQTTDCTQVVGLPSGIPGTGTETPCMS
                     VYQDGGIAGSRTRPDGTFQSLVSNEAGTVFTFTQTPYLPPTAAGGVRALASSATGTGR
                     LLDQNSDGIYETMQVEGTGVPQTRMSLLPRDTTGDGRPDYITVPWTTGGAGLLGVATA
                     TTPQIYFPLTDTNADGWPDTITVQEAGGISTSTGPPLSGAALARAAAVPSASTFGLFF
                     FAAAVVALGVKLLRGTATAL"
     gene            4832..6490
                     /locus_tag="P5164_05550"
     CDS             4832..6490
                     /locus_tag="P5164_05550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY43395.1"
                     /translation="MSLRHPLQGRSASCAALALAATVVAALLLLAPRTTLWDRDESRF
                     ARSAVEMLSSGDFLVPTFAGELRPHKPPLLPWLMALSTSLLGPTELAFRHWSALAHGA
                     SVLLTFAAGRRLAGGKAPLLGAAMIALSPLALLEGAAATADAVLLAGLTGVLAVFADA
                     AAGGVRVRHLVLGALASSIAQLAKGPVAFAVPLLGLGGALLLVPGAFRDRRRLAALLV
                     GSSVLGLLPVLGWGLLADAATNGRLLSAAIGTHVIGRTLAPMEGHGSGFLVGPLYYGA
                     VLLVGFSPFTLFLPPAWVRVRSGGGLAPGARAILLGSVLAPLLLFSVVATKLPHYLLP
                     AFPALALLAAEAAESGERRGLAAGGLLLAPVVVASLSVLAVVVAVPPLAALRAPAAVA
                     IVAISSEVALAVLLLRSGRTRAAAATLLAGTAVALVATAGFGLPALESWKPVPRLAAA
                     ARRAVGDAPAATLGFEEPSVFVYFGPGPVERLASEADAARWARREGPGLLVTTRAGLE
                     RLVAREGLLPLVPVARQGGLNVSKGEPVELIALARGGPWTIAPP"
     gene            6469..7884
                     /locus_tag="P5164_05555"
     CDS             6469..7884
                     /locus_tag="P5164_05555"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HRY43396.1"
                     /translation="MDDRPAVTGVIRRRDDAVAQTPPLGGLPPPEGPSVSGSGTSRFP
                     GFVLGAYGVAAGAAVAAARIALLRKAGASERAERLGTALPPCGPGRLLLHAVSAGEMT
                     AASALASEIERTAGLKLLLTTGTREGRIVAEQVREACRAVEAVAFLPWDRPGAVRRWL
                     ARLAPAAVVTVEAELWPGLFLGARSLGIPVAVASARLRPGEAWRYARAGPFFRPVVDA
                     VSWIGARTEEDRERFLAIGASPSRVEVTGDLKLDAPTPAAALPPGWKVWLEIGPPLLV
                     AASTHAPEEELLLEAVKRLRGEGAEVRLALAPRHVARSGEVERLARQAGFGTCRLSGP
                     PGPASILVVDTFGLLSSLWPQAAIGFLGGTLASVGGHSPVAAAEAGVPLLAGPSLEGV
                     RDVADALRFAGALVDVGKDGAVEELAAALARLLSDEGDRRRRGEAGRRALGSLRGAAA
                     RTATRVLGLLASGRRPGPEAL"
     gene            complement(7925..9694)
                     /locus_tag="P5164_05560"
     CDS             complement(7925..9694)
                     /locus_tag="P5164_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY43397.1"
                     /translation="MSDGILAPGAGRRLLLRIARDAAVERSRFAGAAVALATMAGAQL
                     LLTWLVKEWLEGPLTGGSLLPVRALLLEAAGATLLLAAAVVLSRYLAASVNQRLLERL
                     RDRAARRVLASRALAVRGRPAGELLSRVFSDASSLSGFVETLLKRLVGDGLDALGSIA
                     VAFLVEWRLALGAVVLAPLFGLLLDRLGRVVRRKGSIAQRELGELHAVFSEQLSGLTT
                     IQGYDAAGREADRFATTNATYRRSVLAAELWSATVLASIFLVTGLGFLGAIGWGSRLA
                     LANELTPAGLLAFCLFAARTVEPLRRLADVHATLQRTLAAAARVYEVIDFGFLERQGG
                     TPLPRPVPGRLALEGVRFRHEPSRPLLEGVDLRVEPGETVAVVAASGGGKSTLAAMLA
                     GFLSPDEGRLALDGVDLREADLRDLRRAVRLVEQEPFLFRGTLADNVLFGRPEASRRE
                     VVEALGLCGLESLVAAIPGGIDGSLLEAGRNLSGGQKQRIALARAVLTDPPVLVLDEA
                     TSALDGETEGRLLDDLGPWLARRTVIVMAHRLSTVARYPRVIVLVDGRVAGDGPASRL
                     LESCLPFRALFADQVEPVANRAT"
     gene            complement(9691..10176)
                     /locus_tag="P5164_05565"
     CDS             complement(9691..10176)
                     /locus_tag="P5164_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43398.1"
                     /translation="MDSFPPSGSPRGFDAARAAHPTRMLRIEEVVDLLELTHAAEVIR
                     GYRERMRAGDLFPPVSVVRLFGKWLVADGHKRYVAWTGLGETEIVVEVWPLRRWLADQ
                     AGQARRNARKNGRIAARLFVDPRESLRLARTTAGHWRRVARSLLRRAGRRGRRPESLP
                     A"
     gene            complement(10256..11044)
                     /gene="lpxA"
                     /locus_tag="P5164_05570"
     CDS             complement(10256..11044)
                     /gene="lpxA"
                     /locus_tag="P5164_05570"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRY43399.1"
                     /translation="MGRHRTALVDPGARLGTGVEVGPFAVVEAETAIGDGCELGAHSV
                     VKRYTWMGARNRVHEGAILGGEPQDLGFGGGPSRLEIGDDNRIREGVTIHRATAPGGV
                     TRVGSGCFLMAYSHVAHEDVIGDHVILANNVALAGHVRIDERAFLSGGVVVHQFCRVG
                     RLAMVGGNTKVIKDCLPFVITDGVPARARGLNVVGLRRAGFTASQLRTLKEAYRLLLR
                     SGLPLAEALEKLEALGDPLADELGAFARDSKRGFHRSSREIDLA"
     gene            11255..12361
                     /locus_tag="P5164_05575"
     CDS             11255..12361
                     /locus_tag="P5164_05575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY43400.1"
                     /translation="MKATLLVDRWDASRGGQEGYLAALGGELARRGVALRVLCREGTG
                     RGSGTSVETLVPPGGGAPGERRFLAAAARRLEAREGPVLATRLVAFATHVQLHGGLYA
                     DALDAERESMEGMRRALFGLGTALNGRRRLLLRCERETLAGACPQIMVWTEAQRSRLR
                     DLGVPDGTIRVAPPGVDLDLFSPDPHGFRPDPAREFLFLAHNPRLKGLRSAFRALAVA
                     RRRGLAARLSVVGRVPRDAWRRVAARLGVAEAVSFEGPLSPGGVVRKLRASTGLLHPT
                     FLDPCSLACLEAAACGVPVITTRRNGAVERLGPAGAALVVDDPTDTEGLARAMEEVAS
                     PARHAELREAALGLRPSVGARRHLEDVVDWLGVG"
     gene            complement(12422..>13046)
                     /locus_tag="P5164_05580"
     CDS             complement(12422..>13046)
                     /locus_tag="P5164_05580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY43401.1"
                     /translation="FCGRPGPVRNEALRRVSSPLVAFLDDDDLWLPRRLEAHLAALEA
                     VPAAGLVFGPVRRFGDAEGTWPRRVPPRVDLARLLRGNCVPLSSVLARREALERAGLF
                     PDTAELAQDYELWLRVARSHVLVGHPEPLVRYRVHTGGISRKKALEVEALAAILTRLE
                     SEWDLPPRLLAPARRGLLRSRARLAAGLRQAVRLRALSLAPGPFLRG"
BASE COUNT         1659 a         4800 c         4913 g         1674 t
ORIGIN      
        1 gacgagggcg gcgggggcgt gcgccgcgag gaggttcccg gcgaggcgct cgagcgggag
       61 ggaaggggcg gacgtcgcgc cggccgcgag cgcggcgagc cgggtcccgg cggagagggc
      121 gtgggcgccc acctcgccga ggatgcgggc cccgcgcgcg agcgcggcct cctgttcctc
      181 gagcagaacg acggccgccc cctcggcggg aacgagcggg gaggggcccg agaggagacc
      241 ctcctgctcg agcgcggcga acgcgaacgg gtggaccgcg gagtcggccc cgccggcgag
      301 aacggcgtcg gcgcgccctt cggcgatcca cgcggcccct tcgacgatcg ccgcgaggcc
      361 cgcggcccac cccgtcatca cggtagtgtt cggtccgcgc gcctcctgct ggatcgccac
      421 gtgggcgctg gtcatgttgg ggaggtggac gaggagccag agagggttga ggcccgcgag
      481 ggccgcctcg ccgaacgcgt cggtgtcccg ggccggaagg gccgggtcct ccgcgcgcgc
      541 gagcgcggcg gagagctcct cggcgccgag gtcggagccg ctcgttccga tcgagacgcc
      601 gaggccctcg ctcggccccg cggggaagcc ggcgtcttcg agcgccatcg cggccgcaca
      661 gacggcgaat cgggtcctgc ggtcgcagag cttcagcgcc ttggggacgc ggaagtacgg
      721 gcgggggtcg aggtcgtgca cctcgccggc gagaggcgac gcgaacggcc tcgcgtcgaa
      781 cagcgtggcg ggcgcgacgg cgcaccggcc ctcggcgagc gcccgccgcg tcgccccgag
      841 gccggtgccg aggggtgtga cgaggccgag gccggtgacg acggcgcgcc gctccttcac
      901 gtccccggtc cgtcccgccc ctcgagggcc gcgacttttt cctccagctc cttgatccgg
      961 cgcaggagct ccgggagccg ggcgacgatg gggaagacac gccgggcctc cgccgcggga
     1021 agcgcggggg cgccgtgcca gatcccgggg ccgatgtctc ggtggactcc cgcctgcgcg
     1081 ccgacgaccg tcccctcggc gagggtgacg tggtccgcga tgccggactg tcccgcgagg
     1141 atgctgaggc gcccgacctt cacgcttccg gagaggcccg cctgtccgat gacgcagcag
     1201 tgctcgccga cctgggtgtt gtggccaatc tggacgaggt tgtcgatctt cgtccccgcc
     1261 ccgatcctcg tggcgcccac ggtcgcccgg tcgacgcagg agttcgaccc gatctccacg
     1321 tcgtcggcga tctcgacggt cccgatctga gggaccttgt gctgcactcc gccggcgtcg
     1381 cgctcgtagc cgaagccgtc gctcccgatg acgcttcccg cgtggatccg cacgcggcgt
     1441 ccgaccgtca cgccggggta gaggacgacg ccgggaaaga gaaccgagtc gtcgcccacc
     1501 ttcgcgccct ccccgacgac ggcgaggggg tgaacgcgga cgcgcgcgcc gaggcgggcc
     1561 ccggcctcga cgacggcccc ggggccgacc caggcccctt ctcccatctc cgcgcccggg
     1621 tcgacggacg cgcgcgggtc gaggcccggc ccgcgcgcgt cgtcaggcgc gaagtaggcg
     1681 aggagcgcga cgaacgcccg gcggggatga gggacccgaa tgaccgaacg ccccgacgcg
     1741 gaagtccccg gcgggacgac gagggccgat gcccggcacg cggccatccc ggcgagcgcc
     1801 ttccgatccg cgaggacggc gagcgcgcct tcgcccgctt cctccggagg ctcgagccgg
     1861 tcgatcggtg tctcccggtc gccgtcgatc tcgcctccca ggaactcggc gatgtcgccg
     1921 agcgtcgtct tcctcacgtg ccgctccctt cctcccggac gatggcgccg ccgaaggcgg
     1981 cgccccgctc ggggcgccgc tccgcggcgg ccaggacggc ctcggccgca cgcgcgtcgc
     2041 tgccccccgc gaagaggagg cgcgacacct ccccgagcgc ctgccgctgc ttctcgagaa
     2101 gggactcgtc cccgaggagc cgctccgcag cggccgccag cgcgaagccg ctggcccggt
     2161 cctggatgtg ctcgggaatc gcgcgccgcc cgaggacgag gttcgggagg ccgacggtgt
     2221 cggagtcgat gaggccgagc gagatcgcgc tgcggacgac gcccatcgtc agggccgcga
     2281 gtcggtaggc gatgaccatc ggcgtcccga tgagggcggc ttcgagggag gcggtccccg
     2341 aggcgaggat cgcgaggtcg gccgcgcgga gcgcgtcgtg gccgctggac gtcgttccga
     2401 ggactccctc gagccctcgg cgggccactt cggcctcgat ccggggcgcg aaggcggaat
     2461 cggcgacggg gaggatgaag cggattctcc cgtcccgctc gtgaaggagc cgcgcggcgt
     2521 cgaggaggac gggggtcagt cggcgcacct cgagctcccg gctgcccgga aggaccgcca
     2581 cgagcggtcc ggcggggagt ccgagcgccc cacgggcgtg ccgccgctcc tcttcggtgg
     2641 cgggccgcag gacgtcgccg aggtagtgcc cgacgaaggt cgtggacgcc ccggcggctc
     2701 gccagacgcg ctcctcgtcg gggaacgacg tcaggatctc gtcgaagctc ggtgcgaaga
     2761 cgcgggcgag cgagcgccag atccagacct gcggcgggaa gtaggagagg gtcgggaccc
     2821 cgcgggcccg aagccatcgg ccgagcacca cgtgaaagac gtcgttggcg atgaggacgg
     2881 cggcgtcggg cggcgaaccg gcgacccgcc ggcgcagcga ggcgaacgcg cgcaccaggc
     2941 ccggaacgac ccggaacgac tcgctgatcc ccacggtccc gagggcgttc gtgctccgga
     3001 ggagctcgac gccggctcct gccatccgcg ggccgccgag accgaagaac gcgaggtccg
     3061 aacgacgcgc tctcagcgcg cgcaccagtc gcgagccggc gacgtctccc gacacctctc
     3121 cggcgctgag gaggatcctc acgcgatccc tctccctcgc gcacggagcc gtccggcggc
     3181 ctcgaggagg gcgagctggg cgagcggcac ggcgaccgcg tggagcgcga ggagcctcgc
     3241 ctccgggacg gggcggtcga ccgcccgcgc cacgaggacg cgcgcggcca cgaacgtccc
     3301 cgaaaggaga aggaagaggc ccagcctgcg cttcattccg cggtggagga taagacggtc
     3361 tcgacgaggc ggccgagacc cgtctcgcgc aggattcccg ccctccagcc gtcgaatccg
     3421 gcggaatccg agggcctcgc aacgagatga aagacgagcc tcgcgtcggc gaccttctcg
     3481 tcgccgaccg aggcgcgggt ccgtgccgtc cagacgtctt cgcgggtctc ttccaggtcg
     3541 gcctcgagcc ggatctcgtc gccaggacgg acgaggcgcc ggaacttggc ccgctcgacc
     3601 atcatcagga gggggaaccg gtccggcccg accttcgtgg cgatcgccca cccggccgcc
     3661 tgccccatcg actccgtcag gaggacgccc gggacgatcg ggaacccggg gaagtgatcc
     3721 gcgaagacgg gatcctcggg gcggaacgtc ttccgcgccg tcacacctcg ccccggctcg
     3781 accgagagga actggtcgac gaggacgaac cgcacgacgg gaagggctct cagagggcgg
     3841 tggccgtccc gcgcaggagc ttcacgccga gtgcgacaac agctgcggcg aagaagaaga
     3901 ggccgaacgt cgaagccgac gggaccgcgg cggctctcgc cagcgcggct cccgagagtg
     3961 gcgggccggt cgacgtcgag atcccgcccg cttcctggac cgtgatggtg tcgggccacc
     4021 cgtcggcgtt cgtgtccgtc agcgggaagt agatctgcgg cgtcgtcgcg gtggcgacgc
     4081 cgagaagccc ggcgccgccc gtcgtccacg gcacggtgat gtagtcgggc cgaccgtctc
     4141 ccgtcgtgtc ccgaggaagc aggctcatac gggtctgcgg gactccggtc ccctcgacct
     4201 gcatcgtttc gtagattccg tcggagttct ggtcgaggag ccggcccgtc ccggtcgccg
     4261 aggaggcgag ggcgcgcacc ccgccggccg ccgtgggcgg gaggtacggg gtctgggtga
     4321 acgtgaagac ggtccctgcc tcgttcgaga cgagcgactg aaaggtgccg tccggcctcg
     4381 tgcgcgagcc ggcgatgccg ccgtcctggt agacgctcat gcacggagtc tccgttccgg
     4441 ttccggggat cccggacggg agcccgacga cctgagtgca gtccgtcgtc tgccgctggg
     4501 ggtagcccat ccgctcgccg gcctgcggca ctccgtcccc gttcatgtcg aaccagatct
     4561 gactgctggc gctccacgga ccaggcccgc cggacggtcc cggtgtgggc gtcggtgtgg
     4621 gctgagcccg caatgcgctc gcggaaagaa ggaacgcgag cgcagaggtg gcggcgaagc
     4681 gcgaagtggt tcggctcatg cgggccctcc gaattccggg aacagtcttg agtgccgcaa
     4741 ttgtagcagg tccggagaag gtcagggcgt cgggtcgggc ggatcgaggc acgtgctccg
     4801 gcgcgccttc gctagactcg gccgccgctc catgagcctc cgtcaccctt tacaggggcg
     4861 ttccgcatcc tgcgctgcgc tcgcgctcgc ggccacggtc gtggcggcgc tcctcctgct
     4921 cgcgccgcga acgacgctct gggaccggga cgagtcccgt ttcgcgcgct cggcggtcga
     4981 gatgctttcc tcgggcgact ttctcgtccc gacgttcgcc ggcgagctcc gcccgcacaa
     5041 accgcccctc ctcccctggc tcatggcgct ctcgacgagt ctcctcggcc ccacggagct
     5101 ggccttccgc cactggtccg cgctggcgca cggagcctcc gtcctcctga ccttcgccgc
     5161 cggccggcgc ctcgcgggcg ggaaggcccc gctcctcgga gcggcgatga tcgccttgtc
     5221 gcccctcgcg ctcctggagg gggccgccgc gacggccgac gccgtcctcc tggccgggct
     5281 gacgggagtc ctcgcggtct tcgccgacgc cgcagccggc ggcgtccggg tccggcatct
     5341 cgtcctcggg gcgctggcct cgtcgatcgc gcagctcgcc aaggggcccg tcgcgttcgc
     5401 cgttccgctc ctcggcctcg gcggggcgct cctcctcgtg ccgggggcct tccgcgacag
     5461 gcggcgcctg gccgctctcc tcgtcgggtc ctcggtcctc ggcctcctcc cggtcctcgg
     5521 ctggggcctc ctcgccgacg ccgcgacgaa cggccgcctc ctctcggcgg cgatcgggac
     5581 gcacgtgatc ggccggacgc tcgcgccgat ggaaggccac gggagcggat tcctcgtggg
     5641 gccgctctac tacggcgccg tccttctcgt cggcttctcc ccgttcacgc tcttcctgcc
     5701 ccccgcctgg gtgcgcgtcc ggtcgggcgg cggcctcgcg cccggggccc gcgcgatcct
     5761 cctcggaagc gtcctcgccc cgctgcttct cttcagcgtc gtcgccacca agctcccgca
     5821 ctacctcctc ccggcgttcc cggccctcgc cctgctcgcg gccgaggccg cggaatcggg
     5881 cgaacggcgc ggcctcgccg ccggcgggct cctcctcgct ccggtcgtcg tcgcgtcgct
     5941 ctcggtcctc gccgtcgtcg tggccgtccc cccgctggcg gcattgcggg ctccggcggc
     6001 tgtggccatc gtcgcgatct cgtcggaggt cgccctggcc gtgctgctcc tccggtcggg
     6061 acgtacgcga gccgccgccg cgacgcttct cgcgggaacg gccgtcgcgc tcgtcgcgac
     6121 ggcggggttc ggcctccccg cgctcgagag ctggaagccg gtgccccgcc tcgcggcggc
     6181 ggcgcggcgc gccgtgggag acgcccccgc cgcgacgctc gggttcgagg agccgagcgt
     6241 cttcgtctat ttcggcccgg gacccgtgga gcgcctcgcg agcgaggcgg acgccgcccg
     6301 ctgggcgcgc cgcgaaggcc cgggtctcct cgtcacgacg cgcgccggtc tcgaacgcct
     6361 ggtggcgcgc gagggtctgc tgcccctcgt cccggtggcg cggcagggag ggctgaacgt
     6421 ctcgaaaggg gagcccgtcg agctgatcgc tctggcgcga ggtggaccgt ggacgatcgc
     6481 cccgccgtga ccggcgtcat ccggcgccgg gacgacgccg tcgcccagac gccgccgctc
     6541 ggaggtctgc cccctcccga gggaccgtcc gtgagcgggt ccggcacctc ccggtttccc
     6601 ggcttcgtcc tcggtgcgta cggagtggcc gccggggcgg cggtcgccgc ggcgcgaatc
     6661 gcgctccttc gcaaggccgg agcttccgag cgggcggagc gcctcggcac ggcgctgccg
     6721 ccgtgcggcc cggggagact tctcctccac gccgtctcgg cgggggagat gaccgccgcg
     6781 tcggccctcg cgtcggagat cgagcggacg gccgggctga agctgcttct gacgaccggg
     6841 acgagagagg gacggatcgt ggccgagcag gttcgcgagg cgtgccgcgc ggtcgaggcg
     6901 gtcgcgttcc tgccgtggga ccggccgggt gcggtgcgcc gttggctcgc acgcctcgct
     6961 cccgcggccg tggtgacggt cgaggccgag ctctggcccg ggctcttcct cggggcgcgg
     7021 tctctcggca tcccggtcgc cgtcgcgagc gcgcggctcc gcccgggcga ggcgtggcgg
     7081 tacgcgcggg cgggcccgtt cttccgcccg gtcgtcgacg cggtgtcctg gatcggcgcg
     7141 cgaacggagg aggaccgcga gagattcctc gcgatcggcg cgagcccttc ccgcgtcgag
     7201 gtgaccggcg acctgaagct cgacgccccg acgccggccg cggcgctgcc gcccgggtgg
     7261 aaggtctggc tcgagatcgg ccctcctctc ctcgtcgcgg cgagcaccca cgctcccgag
     7321 gaggagctcc tcctggaggc cgtaaagagg ctgcgcgggg aaggcgccga ggtccggctg
     7381 gccctcgcgc cgcggcacgt cgcgaggtcg ggcgaggtcg agcgcctcgc ccgccaggcc
     7441 ggcttcggga cgtgccggct ctcgggccct cccggccccg catcgatcct cgtcgtcgac
     7501 accttcggac tgctctcctc gctctggccg caggccgcga tcggcttcct cggcgggacc
     7561 ctcgcatcgg tcggcgggca cagccccgtc gcggccgcgg aggcgggcgt gccgctcctg
     7621 gccgggcctt cgctcgaagg ggtccgcgac gtggccgacg ccctccgatt cgccggagcg
     7681 ctcgtcgacg tgggaaagga cggggccgtc gaggagctcg cagcggcgct ggcgcggctc
     7741 ctctccgacg agggcgatcg ccgtcgccgg ggcgaggccg ggcgccgcgc tctcggttcc
     7801 ctccgaggcg ccgccgcgcg aacggcgacg agggtcctcg gtcttctcgc ctcggggcgg
     7861 cgtcccggac ccgaggccct ctgacggctc cggcccggac tcgcgggggc gaccccgctc
     7921 ccgctcacgt cgcgcggttc gcgacgggct cgacctgatc cgcgaagagc gcccggaagg
     7981 ggagacacga ctccagcagg cgcgaggccg gaccgtctcc cgcgacgcgg ccgtcgacga
     8041 ggacgatcac cctcgggtat cgggccacgg tggagagacg gtgcgccatc acgatcaccg
     8101 tccgtcgcgc gagccacggc ccgagatcgt cgaggagccg gccctccgtc tcgccgtcga
     8161 gcgcgctcgt cgcctcgtcg aggaccagga ccggagggtc ggtgaggacg gcccgcgcga
     8221 gggcgatcct ctgcttctgg ccgcccgaga ggtttcgccc ggcctcgagc agcgagccgt
     8281 cgatcccgcc ggggatcgcg gcgacgaggc tctcgaggcc gcagaggccc agcgcctcga
     8341 cgacctcgcg acgcgacgcc tccgggcggc cgaagaggac gttgtccgcg agcgtccccc
     8401 ggaagaggaa cggctcctgc tcgacgagcc tcacggcgcg cctcaggtcg cgaaggtccg
     8461 cttcacgaag gtccacgccg tcgagcgcga gcctcccctc gtccggcgag aggaaccccg
     8521 cgagcatcgc cgcgagagtg ctcttccctc cgccgctggc cgcgacgacg gcgaccgtct
     8581 ctcccggctc gacccggagg tcgactccct cgaggagcgg gcgcgacggc tcgtggcgga
     8641 aacggacacc ctccagcgcg agcctccccg gcacgggccg cgggaggggc gtcccgccct
     8701 gacgttcgag gaagccgaag tcgatcacct cgtagacccg cgcggccgcg gcgagggtcc
     8761 gctggagcgt cgcgtggacg tcggcgagac gccggagcgg ctcgaccgtc cgagcggcga
     8821 agaggcagaa cgccaggaga ccggccggtg tcagctcgtt cgcgagggcg agccggcttc
     8881 cccagccgat cgcaccgagg aacccgagtc cggtcacgag gaagatcgag gcgaggaccg
     8941 tcgcggacca gagctcggcg gccaggacgc tgcggcgata ggtggcgttc gtcgtcgcga
     9001 agcgatccgc ctcgcgcccg gccgcgtcgt atccctggat ggtcgtcagg cccgagagct
     9061 gctcggagaa gacggcgtga agctctccca gctccctctg ggcgatgctc cccttgcgcc
     9121 tgacgacgcg tcccaggcgg tcgagaagga gtccgaaaag gggggccagg acgacggccc
     9181 cgagggcgag gcgccactcg acgaggaagg ccacggcgat cgagccgagg gcgtcgaggc
     9241 cgtcgccgac gagcctcttc agcagggtct cgacgaaccc cgagaggctg ctggcgtcgg
     9301 agaagacccg cgagaggagc tcgccggccg ggcggcccct caccgcgagg gcccggctcg
     9361 cgaggacccg ccgcgccgct cggtcgcgca gccgctcgag gaggcgctgg ttgacgctcg
     9421 ccgcgaggta gcgcgagagg acgacggccg ccgcgaggag gagggtcgcg cccgcggcct
     9481 cgaggagaag ggcgcgaacg gggaggagcg agcctcccgt gagcggcccc tccagccact
     9541 ccttgacgag ccaggtcagg aggagctggg cgccggccat cgtcgcgagc gcgaccgccg
     9601 cccccgcgaa tcgggaacgc tcgaccgccg cgtccctcgc gatccgcagg agcaggcgtc
     9661 gtccggcacc cggtgccagg atcccgtcgc tcacgccgga aggctctcgg gtcgcctgcc
     9721 gcgccggccg gcccgccgca gcagggagcg ggccacgcgc cgccagtgcc ccgccgtggt
     9781 ccgggccagt cgcagcgact cgcgcgggtc gacgaagagg cgggcggcga tcctcccgtt
     9841 cttccgcgcg ttgcgcctcg cctggccggc ctggtcggcc aaccagcgcc ggagcggcca
     9901 gacctcgacg acgatctcgg tctctccgag ccccgtccac gccacgtagc gcttgtgccc
     9961 gtcggcgacg agccacttcc cgaagagccg cacgacggag accggcggga agaggtcccc
    10021 cgcgcgcatc ctctcgcgat acccgcggat cacctccgcc gcgtgcgtca gctcgaggag
    10081 gtcgacgacc tcctcgattc ggagcatccg cgtcgggtgg gccgcacgcg cggcgtcgaa
    10141 gcccctcggg ctcccggagg gcgggaagct gtccaccgtg gccggaccat gccggtacgc
    10201 catgcagtcc gtcaaggccg caccggtcgg gcgggggacg ccggcagccg gcctgctagg
    10261 cgaggtcgat ctcgcgcgac gagcggtgga agccccgctt cgagtcgcgc gcgaaggcgc
    10321 ccagctcgtc cgccagaggg tcgccgagcg cctccagctt ctcgagggcc tcggcgagcg
    10381 gcaggcccga gcgcaggagg agccggtagg cctccttcag cgtccggagc tgcgaagccg
    10441 tgaagccggc ccgccggagg ccgacgacgt tcaggccgcg cgcgcgggcc ggcacgccgt
    10501 cggtgatcac gaaggggagg cagtccttga tgaccttcgt gttcccgccg accatcgcga
    10561 ggcgccccac ccggcagaac tggtgcacca caaccccgcc cgagaggaag gccctctcgt
    10621 cgatccgcac gtgcccggcc agcgcgacgt tgttcgccag gatcacgtgg tcgccgatca
    10681 cgtcctcgtg ggcgacgtgg gagtacgcca tgaggaagca gccggagccg acgcgcgtca
    10741 cgcccccggg ggccgtcgcg cgatggatcg tcactccctc gcgaatccgg ttgtcgtccc
    10801 cgatctccag gcggctcggg cctccgccga agccgaggtc ctggggctcg ccgccgagga
    10861 tcgctccctc gtggacgcgg ttgcgggcgc ccatccacgt gtatcgcttg acgacgctgt
    10921 gggcgcccag ctcgcacccg tcgccgattg ccgtctcggc ctcgacgacc gcgaaaggcc
    10981 cgacctccac cccggtcccg agccgcgcgc cgggatcgac gagagccgtg cggtgccttc
    11041 ccatgcggcc gggattctag tttgcgtccg gcccgcgaga ggcgagcctc ggaaggaatt
    11101 gccgaggccc ggagggaagt gagacagtcg cttgaaggcg gtgccctgcg gatgcgccga
    11161 aggagaggcg aggacggtcg cgcatgctcg gggagatgac gacggatcgt tgccacgtgc
    11221 cggcccccgg gtcccgtgcc gaacgcgggc gcgcgtgaag gcgacccttc tcgtcgaccg
    11281 ctgggacgcc tcgcgcggag ggcaggaggg atacctcgcc gcgctgggcg gtgagctcgc
    11341 gcggcgaggg gtcgccctcc gggtgctctg tcgcgagggc accggaagag ggtccgggac
    11401 ctccgtcgag acgctcgttc ccccgggagg aggcgcgccc ggcgaacggc gtttcctggc
    11461 ggccgccgcg cgccggctcg aggcccgaga ggggcccgtc ctcgcgacgc ggctcgtcgc
    11521 gttcgcgacg cacgtccagc tgcacggcgg cctctacgcc gacgccctcg atgcggagcg
    11581 cgagtcgatg gaggggatgc ggcgcgcgct cttcggcctc gggacggccc tcaacgggcg
    11641 acgccggctt ctccttcgct gcgagcgaga gaccctcgcg ggcgcctgcc cgcagatcat
    11701 ggtctggacg gaggcgcaaa ggagccgcct gcgcgacctg ggcgtgcccg acgggacgat
    11761 ccgggtggca ccgcccggcg tcgacctcga cctcttctcg ccggatccgc acggcttccg
    11821 accggatccc gcccgggagt tcctcttcct cgcccacaac ccccgtctca agggcctgcg
    11881 ttccgcgttc cgggccctcg ccgtcgcgag gaggcgtggc ctcgccgcgc ggctttccgt
    11941 cgtggggcgg gtgccgcgcg acgcgtggcg ccgtgtcgcg gcccgcctcg gggtcgcgga
    12001 agcagtctcg ttcgaaggcc cgctctctcc cgggggcgtc gttcgaaagc tccgcgcgtc
    12061 gaccggcctc ctccacccga cgttcctcga tccctgctcg ctggcctgtc tcgaggcggc
    12121 cgcctgcggg gtgcccgtga tcacgacccg gaggaacggt gcggtcgaga ggctcgggcc
    12181 ggcgggggcg gcgctcgtcg tggacgatcc gacggacacg gaggggctcg cgcgggcgat
    12241 ggaggaggtc gcgagcccgg cccggcacgc ggagctccgg gaggccgccc tcggcctccg
    12301 gccgtcggtc ggcgcgcggc gtcacctgga ggacgtggtc gactggctcg gcgtcggctg
    12361 acgccgcggt gcccgagccg gacggaggac gtcggggttc ttcccccccc tcgagacggc
    12421 ctcagccgcg aaggaacggt cccggcgcga gagagagggc gcggagccgg accgcctgcc
    12481 gcaggccggc ggcgagccgg gcacgggagc ggagaaggcc gcggcgcgcg ggggcgagga
    12541 gccgcggcgg caggtcccac tccgattcca ggcgcgtgag gatcgcggcc agggcctcga
    12601 cctcgagggc cttcttccgg ctgatcccgc ccgtgtggac gcggtagcgg acgaggggct
    12661 cgggatgccc gacgagaacg tgcgatcgcg cgacgcgaag ccagagctcg tagtcctggg
    12721 ccagctccgc cgtgtcgggg aagaggccgg cccgttcgag cgcttcccgg cgcgcgagga
    12781 cggaggagag cgggacacag ttccctcgca gaagccgggc gaggtcgacc cgagggggaa
    12841 cgcgccgggg ccacgttccc tcggcgtcac cgaaacgccg cacggggccg aagaccagcc
    12901 cggcggccgg aacggcctcc agagcggcca ggtgcgcctc gagccggcgg ggaagccaga
    12961 ggtcgtcgtc gtcgaggaac gcgacgagag gcgacgagac gcggcggagg gcctcgttcc
    13021 gcacggggcc gggccttccg cagaag
//
LOCUS       DAOTEZ010000071        12947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3376_length_12947_cov_5.797083, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000071 DAOTEZ010000000
VERSION     DAOTEZ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12947
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3376_length_12947_cov_5.797083"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..286
                     /gene="rpmG"
                     /locus_tag="P5164_05585"
     CDS             131..286
                     /gene="rpmG"
                     /locus_tag="P5164_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRY43402.1"
                     /translation="MPRDLISLACGECKRRNYTTTKNKKTHQEKLEVSKYCRFCRKHT
                     AHKEGKV"
     gene            301..376
                     /locus_tag="P5164_05590"
     tRNA            301..376
                     /locus_tag="P5164_05590"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:334..336,aa:Trp,seq:cca)
     gene            457..669
                     /gene="secE"
                     /locus_tag="P5164_05595"
     CDS             457..669
                     /gene="secE"
                     /locus_tag="P5164_05595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRY43403.1"
                     /translation="MNLTERWKTFREFLVDVRKETGKVSWPAKDEVVGTTTVVILYTV
                     VVGVFLFAVDATITPLINWFFAAFGQ"
     gene            746..1273
                     /gene="nusG"
                     /locus_tag="P5164_05600"
     CDS             746..1273
                     /gene="nusG"
                     /locus_tag="P5164_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRY43404.1"
                     /translation="MDWYIVHTYSGFEDRVVQELTNRIKAQGMESQFGEIRVPKETVV
                     EMKAGKRRNVERKFFPGYVLVQMEMADETWHLVRNTPKVTGFVGGSSKSPVPLMPEEV
                     EAILHHTAESKEKPKPKYTYERGEKVKIVDGPFKEFTGDVEEINIERSTLRVLVTIFG
                     RPTPVELEFVQVQKL"
     gene            1346..1771
                     /gene="rplK"
                     /locus_tag="P5164_05605"
     CDS             1346..1771
                     /gene="rplK"
                     /locus_tag="P5164_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRY43405.1"
                     /translation="MAKKVVGQVKLQIEAGKATPAPPVGTALGPQGVNIMDFCKSFNA
                     RTAKDAGLIIPVVVTVYSDRSYSFILKTPPASVLLKKAAKVEKGSGVPNKNRVGKVTM
                     QQVEEIAKLKLVDLNAADLEAAKKTVAGTARSMGIEVIG"
     gene            1932..2642
                     /gene="rplA"
                     /locus_tag="P5164_05610"
     CDS             1932..2642
                     /gene="rplA"
                     /locus_tag="P5164_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005038373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRY43406.1"
                     /translation="MTEGKKYTAAAAKIDRTKLYDVQQAVALVRSAAYAKFNETLEVA
                     LRLGVDPKHADQMVRGTVVLPHGTGQTVRVAVVASGEKVREAEDAGADVVGGDDLVAK
                     IQGGYLDFEALVATPDMMKSLGRLGKVLGPRGLMPNPKAGTVTFDVGKAVKELKAGKI
                     EFRVDKTAIIHCPVGKLSFADEALVENTEALIAAIQKAKPAAAKGKYVKSIFLGSTMG
                     PSVPVDPAVADKVAGRAA"
     gene            2639..3163
                     /gene="rplJ"
                     /locus_tag="P5164_05615"
     CDS             2639..3163
                     /gene="rplJ"
                     /locus_tag="P5164_05615"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRY43407.1"
                     /translation="MNRTEKTDAIAQMTEALGTAPHAFLIDFQGISVPDVTELRRQIR
                     ATGSEYVVVKNTLALRAIKDAPIGALSEHFTGMTAVAWSKTDIVQLAKVLHQFGKTNP
                     KVKVKAALVEGRPVPAAALESLASLPTRPELVAKLLGLMQSPVRRLVTVLSSPQRKLA
                     MTISAVAEKKSAAA"
     gene            3234..3614
                     /gene="rplL"
                     /locus_tag="P5164_05620"
     CDS             3234..3614
                     /gene="rplL"
                     /locus_tag="P5164_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005889295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRY43408.1"
                     /translation="MALSVEQFVSEVEGMSVLELNNLVKALEEKFGVSAAMMAAPAAA
                     AAPAAAAAPAEEQTEFTVTLKEAGGNKINVIKAVREVNSSLGLKEAKDLVENLGVLKE
                     SVSKDEAAAIKKKFEEIGAKVEIK"
     gene            3770..8044
                     /gene="rpoB"
                     /locus_tag="P5164_05625"
     CDS             3770..8044
                     /gene="rpoB"
                     /locus_tag="P5164_05625"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRY43409.1"
                     /translation="MADRMLAPRQTARHDFGKIKTFLPLPNLIDVQRRSYEDFLQMDL
                     LPHERDDVGLEAVFRGVFPFSDFRETCALEYVSFRIGDWASRSGRLEGIQHLRFTCEH
                     CGETVRVKDPRANVVECQACGHPNPSKVTIDEVCGTPVELRLPFTVAECQERGLTYAV
                     PLKVTFRLKVFDKDNAPTGKSAPKEWPIRDIKEEEVYFGDIPLMTDNGTFIINGTERV
                     IVSQLHRSPGVFFTREGAHTLIGKIIPYRGSWVEFEIDSKGLFGVRIDRKRKFPGTVF
                     LRALGLESDEQILKKFYSAVGLTFDKGKAHLVLPPDVLAKEEIRQKHVRGVRKDGRIL
                     ADVKLTADQREQLASGQSVTVTADPAKLGRILFAVDVVDLSSGEVLYEAGTEVPDDLA
                     TVMAEKQATPVEVLFPDWEPIGETLIATLRKDSVKTKREALLEIYKRMRPGDPPTDES
                     AKNLFVGMFFDPRKYDFSRVGRYKFNIRMGLSTSLEQKTLSSDDFYVVIDALLKLRKD
                     IGKTDDIDNLGNRRVRCVGELLENQFRIGLVRMERAIKEKMSVHQDIDSAMPHDLINS
                     KPVIAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRLSALGPGGLTRERAGFEVRDVHAT
                     HYGRICPIETPEGPNIGLISSLSTYARISEYGFIESPYKKVRDGRVLEHFEIFTVGDS
                     GWKKGAIVEASELEAMNAKLKKGGKRLAVGRPTAFYLPAWEEEDYAIAQANEPLEADG
                     RFANDRVQVTLQGEFSAKPRETVHFMDVSPKQLVSVAAALIPFLEHDDANRALMGSNM
                     QRQSVPLLRTEPPIVGTGLEGVVARDSGAVVLCKRDGIVDTVDARRIIVRVEGIGPDG
                     KPREFGADIYPLLKFRRSNQNTCINQKPIVKEGQRVRAGEVLADGPCTSGGELALGRN
                     ILVAFMPWRGYNFEDAILISEKMVKEDYYTSIHIEEFEIEARETKLGPEEITKDIPNV
                     SEHALRDLDESGIIRIGANVKPGDILVGKVTPKGETQLTPEEKLLRAIFGEKAGDVRD
                     ASLKCPPGIEGVVVDVKIFTRKDDEKQKDVRAKQILDEEIGFLNKDAEDEIRIVRTEA
                     KEKIERLLEGRTVLEDVVDPQGNVLVAANHKLTVEKIGMMGPRLLKSLKLRDEEIKDT
                     VRLVLQRADSQVDVIRKVAADRVTLLSKGDELAPGVNKTVKVYVAMKRKLQVGDKMAG
                     RHGNKGVISRILPEEDMPYLPDGTPVQIVLNPLGVPSRMNVGQILETHLGWAAQALGL
                     SIASPVFDGASEEEIRALLEKSNLPRSGKTELYDGMTGERFEQKVTVGYIYMLKLSHL
                     VDDKIHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQELLTVKSD
                     DVDGRSRVYEAIVKGEVPEEPGLPESFNVLVRELQSLCLDVELLKV"
     gene            8055..12278
                     /gene="rpoC"
                     /locus_tag="P5164_05630"
     CDS             8055..12278
                     /gene="rpoC"
                     /locus_tag="P5164_05630"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005038366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRY43410.1"
                     /translation="MTMDTFGASPLSPAVPAFRGPGPRDFNAIKIALASPEKIRSWSF
                     GEVTKPETINYRTFKPERDGLFCAKIFGPITDWECLCGKYKRMKHRGVVCDKCGVEVT
                     KSKVRRERMGHIELAAPVSHVWFFKGLPSRIGHLLDLTLRDLERILYFEAYVVVDPGP
                     LAEEENVNEKVLITEERYRDLRAKYGADAFVAKMGAEAIQELLQRVNIDDLADELRLI
                     MKTETSALKRLKAAKRLKVVDAFRRSGHRPEWMILNVIPVIPPELRPLVPLDGGRFAT
                     SDLNDLYRRVINRNNRLKKLLELRAPEVIVRNEKRMLQEAVDALFDNGRRGRVLKGTN
                     NRPLKSLSDTLKGKSGRFRQNLLGKRVDYSGRSVIVVGPELKLNQCGLPKRMALELFK
                     PFIYRRLEQKGLTSTIKMSKELVEAAPNEVWDALEEVIKEHPVLLNRAPTLHRLGIQA
                     FEPVLIEGKAIKIHPLVCAAFNADFDGDQMAVHVPLSPKAQVEAQVLMLSAHNILSPA
                     SGKPLTVPSQDLVLGLYYLTRRKEGRKGEGRVFAGFDDVLLAHGAGELDTHAGITVRY
                     TGRLIDLENQVIGDQQDIVHAEPIDCHGTRIETTVGRVLMCAHLPKDIPFINGTLKKK
                     GLQDLVNYCFVNLGLERTVEILDIIKQLGFAAATRAGISIGVDDMRIPSKKTGFVDKA
                     RKDVLEVDNQRAQGAITDGERHNKIIDIWHRATEAVSEAMMKEMKAEDAAPGNEFNPI
                     FMMADSGARGSKEQVRQLAGMRGPMSKPSGEVMEQPIISNFREGLSVLEYFISTHGAR
                     KGLADTALKTADSGYLTRRLVDVAQDVIINEEDCATLDGITVTAIVEGGEILEPLRDR
                     IVGRFVQEDVYDPATDEVVIAANDLITEEIATAIQEAGIEKVKIRSVLTCETRRGVCR
                     KCYGRNLATGRMVDIGEAVGVIAAQSIGEPGTQLTMRTFHYGGTARVSETARHQAKNP
                     GVARFLNALTVRKSDGTEVVVNRNSKLVIQDAKGREKERYSLAYGSVLKVTSGEEVKP
                     GQLLVTWDPFASPILSEIEGKVVFKDILEGENLREETDKVTGLSQKIVVETTGTTEKR
                     APQLVVLGVDGTERRYALPIPSHLMVSDGDTVTPGDALAKKPVEKKRTRDIVGGLPRV
                     VELFEARRPKEPAVITEVSGAVRHAGTVKGQQKLVVESEDGQKRDILVPRGAHVMVQE
                     GERVEAGDPLTEGDPSPHDILAVKGEKELQRYMTDKIQEVYRSQGVGINDKHIEVIVR
                     QMMRSVRVEEVGDTEFLIDEQVDKFRFREENDRIIAAGGKPAQGRPLLLGITKASLST
                     DSFVSAASFQETTRVLTEASIAGRVDLLRGLKENVIVGRLIPAGTGMDFYRNVELERE
                     EIPIQPDEIEEPFYDDEPPVVAADLDEEI"
     gene            12590..>12947
                     /gene="rpsL"
                     /locus_tag="P5164_05635"
     CDS             12590..>12947
                     /gene="rpsL"
                     /locus_tag="P5164_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRY43411.1"
                     /translation="MPTISQLVRKGREAVRYKTKSPALQSCPQRRGVCTQVKTTTPKK
                     PNSALRKIARVRLTNGIEVTTYIPGIGHNLQEHSIVMIRGGRVKDLPGVRYRIIRGTL
                     DAVGVANRKQSRSKYGA"
BASE COUNT         2399 a         4488 c         4285 g         1775 t
ORIGIN      
        1 tagagtcaca gccttccaag ctgttggtcg cgggttcgaa tcccgtctcc cgctccaccc
       61 catccccggg gcggggacgg aacggggagc cgggacgagc cgcgggggcg gcgaggaagc
      121 ggagaaagcg atgccgagag acctgatcag cctggcgtgc ggcgagtgca agcgccggaa
      181 ctacacgacg acgaagaaca agaagaccca ccaggagaag ctggaggtct cgaagtactg
      241 ccgtttctgc cgcaagcaca cggcgcacaa ggaaggcaag gtctagggaa ggacgcgcac
      301 aggggtgtag ctcaattggc agagcaacgg tctccaaaac cgtaggttcg gagttcgagt
      361 ctccgcaccc ctgccacatc ccccgcctcg ccagggcggg ccgaacgccc cggggcgggc
      421 ggcgcggcgc tcgagggcgc caggaagacg gatcgaatga acctcaccga gcggtggaag
      481 accttcaggg agtttctcgt cgacgtccgg aaggagaccg gcaaggtcag ctggcctgcg
      541 aaggacgagg tcgtcggaac gacgaccgtc gtgatcctct ataccgtcgt cgtcggcgtg
      601 ttcctcttcg cggtcgacgc gacgatcacg ccgctcatca actggttctt cgccgccttc
      661 gggcagtgat cccgggaggc gcgccgcgat gatcgagaac gacggatccc cggccctctc
      721 cgaagcgtcc tccccggcgc ccgcgatgga ctggtacatc gtccacacgt actcgggctt
      781 cgaggaccgc gtcgtccagg agctgacgaa ccggatcaag gcccagggga tggagtcgca
      841 gttcggcgag atccgcgtgc cgaaggagac cgtcgtcgag atgaaggcgg ggaagcgccg
      901 caacgtcgag cgcaagttct tccccggcta cgtcctcgtc cagatggaga tggccgacga
      961 gacgtggcac ctcgtgagga acacgccgaa ggtcaccggc ttcgtcggag gctcctcgaa
     1021 gagccccgtc ccgctcatgc cggaggaggt cgaggcgatc ctccaccaca cggcggagtc
     1081 gaaggagaag ccgaagccga agtacaccta cgagcgtggc gagaaggtga agatcgtcga
     1141 cggccccttc aaggagttca cgggggacgt cgaggagatc aacatcgagc gttccacgct
     1201 ccgcgtcctc gtcacgatct tcggccggcc gacgcccgtc gagctcgagt tcgtccaggt
     1261 ccagaagctg taatccgcgg gggccggcgc gccggcctcc cgaagaacgc ggggctcgcc
     1321 ccgtcaccac tcgaaggaac ccacaatggc caagaaagtc gtcgggcagg tcaagctcca
     1381 gatcgaagcc ggaaaagcga ctcccgcgcc gcccgtcggc accgccctcg gcccgcaggg
     1441 cgtcaacatc atggacttct gcaagtcctt caatgcgagg acggcgaagg acgcggggct
     1501 gatcatcccg gtcgtcgtga cggtctactc cgaccggtcc tactccttca tcctgaagac
     1561 cccgccggcc tccgtcctcc tcaagaaggc cgcgaaggtg gagaagggct cgggcgtccc
     1621 gaacaagaac cgggtcggga aggtcacgat gcagcaggtc gaggagatcg cgaagctgaa
     1681 gctcgtcgac ctcaacgccg ccgacctcga ggccgccaag aaaacggtcg cggggacggc
     1741 tcgctccatg gggatcgagg tcatcggctg agaagaacga aatcgtcgcg tgacgatttc
     1801 tcattgagac ggcgaacggg ccggcgccgc ccctctcacg gcgcgcggct ccgcgatgcc
     1861 cgggctgcct cccggggctt ctcatcgcgg gggcgcagga cgatcgccgc actcggaaga
     1921 gcggaagaga catgacggaa ggcaagaagt acaccgccgc ggcagccaag atcgaccgga
     1981 cgaagctcta cgacgtccag caggccgtcg cgctcgtccg ttcggcggcg tacgcgaagt
     2041 tcaacgagac gctcgaggtg gccctgcgcc tcggcgtcga cccgaagcac gcggaccaga
     2101 tggtccgcgg cacggtcgtc ctcccgcacg gaacgggtca gaccgtccgc gtggccgtcg
     2161 tcgcttccgg cgagaaggtc cgcgaggcgg aggatgccgg cgccgacgtc gtcggagggg
     2221 acgacctcgt cgcgaagatc cagggcgggt acctcgactt cgaggccctc gtcgccaccc
     2281 ccgacatgat gaagtccctc gggcggctcg gaaaggtcct cggcccccgc ggcctcatgc
     2341 cgaacccgaa ggccggcacg gtcaccttcg acgtcggcaa ggccgtgaag gagctgaagg
     2401 ccggcaagat cgagttccgc gtcgacaaga ccgcgatcat ccactgcccg gtcggcaagc
     2461 tctcgttcgc ggacgaggcc ctcgtggaga acacggaggc cctgatcgcg gcgatccaga
     2521 aggcgaagcc ggccgccgcc aaggggaagt acgtgaagtc catcttcctc ggctcgacga
     2581 tggggccgag cgtgccggtc gaccccgcgg tcgccgacaa ggtcgccggg agggccgcgt
     2641 gaaccgcacc gagaagaccg acgccatcgc gcagatgacg gaagccctgg ggaccgctcc
     2701 ccacgccttc ctcatcgact tccaggggat ctcggtcccc gacgtcaccg agctgcgccg
     2761 tcagatccgc gcgaccggct cggagtacgt cgtcgtcaag aacacgctcg ccttgcgcgc
     2821 catcaaggac gccccgatcg gcgcgctctc cgagcacttc accggcatga cggcggtcgc
     2881 ctggtcgaag accgacatcg tccagctcgc gaaggtcctc caccagttcg ggaagacgaa
     2941 cccgaaggtc aaggtgaagg ccgccctcgt cgagggacgg ccggtcccgg ccgcggccct
     3001 cgagtcgctc gccagcctcc cgacgcgccc cgagctcgtg gcgaagctcc tcggcctcat
     3061 gcagtccccg gtgcgccgcc tcgtcaccgt cctctcgtcg ccgcagcgca agctcgcgat
     3121 gacgatctcg gccgtggccg agaagaagag cgccgcggcc tgaggcccgg cgcgcccgaa
     3181 tcgcccgtag aaacgcgcac gcgcgaagag acaaggaaag caaggagaac gaaatggccc
     3241 tttccgtcga gcagttcgtc agcgaagtcg aggggatgtc cgtcctcgag ctcaacaacc
     3301 tggtcaaggc cctcgaggag aagttcggcg tctccgcggc catgatggcc gccccggccg
     3361 ccgccgccgc cccggccgcc gccgccgccc cggccgagga gcagaccgag ttcaccgtca
     3421 ccctcaagga ggccggcggc aacaagatca acgtcatcaa ggcggtccgc gaggtcaact
     3481 cctccctcgg cctgaaggag gccaaggacc tcgtcgagaa cctcggggtc ctcaaggagt
     3541 ccgtctcgaa ggacgaggcg gcggccatca agaagaagtt cgaggagatc ggcgccaagg
     3601 tcgagatcaa gtagtccggc gtgtcccgca acgggaggac cgtccggtga cggaggtcga
     3661 ttccccgggg ggccggctcg tccggccccg cggggatcgc gccgtctcgc cgggcggtat
     3721 ttccctcttt ccgcgtcacc ctcttcccgg atgaagggag cccgctcgaa tggctgaccg
     3781 catgctcgcc ccgaggcaga ccgcccggca cgatttcggc aagatcaaga cgttcctgcc
     3841 cctgccgaac ctgatcgacg tccagaggcg gtcctacgag gacttcctcc agatggacct
     3901 cctcccgcac gagcgggacg acgtcggtct ggaggccgtc ttccgcgggg tcttcccgtt
     3961 ctccgacttc cgggagacgt gcgcgctcga gtacgtgtcg ttccggatcg gcgactgggc
     4021 ctcccggagc gggcggctcg aggggatcca gcacctccgg ttcacctgcg agcactgcgg
     4081 cgagacggtc cgggtgaagg acccgcgggc gaacgtggtc gagtgccagg cctgcggcca
     4141 cccgaacccg agcaaggtga cgatcgacga ggtctgcggg accccggtcg agctgcgcct
     4201 cccgttcacg gtggcggagt gccaggagcg cggcctcacg tacgcggtcc ccctgaaggt
     4261 cacgttccgg ctgaaggtct tcgacaagga caacgccccg accgggaaga gcgccccgaa
     4321 ggagtggccg atccgggaca tcaaggagga ggaggtctac ttcggcgaca tccccctcat
     4381 gacggacaac gggaccttca tcatcaacgg gacggagcgc gtcatcgtct cgcagctcca
     4441 ccgctcgccg ggcgtgttct tcacccgcga aggggcccac accctgatcg gaaagatcat
     4501 cccgtaccgc ggctcctggg tggagttcga gatcgactcc aagggcctct tcggcgtccg
     4561 gatcgaccgg aagcggaagt tccccgggac ggtcttcctc cgcgcgctgg gcctcgagag
     4621 cgacgagcag atcctcaaga agttctactc ggccgtcggc ctcacgttcg acaaggggaa
     4681 ggcgcacctc gtcctcccgc cggacgtcct cgccaaggag gagatccggc agaagcacgt
     4741 ccgcggcgtc cggaaggacg ggcggatcct cgccgacgtc aagctcaccg ccgaccagcg
     4801 ggagcagctc gcctccggcc agagcgtcac ggtcacggcc gacccggcca agctgggccg
     4861 gatcctcttc gccgtcgacg tcgtcgacct ctcctcgggc gaggtcctct acgaggccgg
     4921 cacggaggtc cccgacgacc tcgcgaccgt gatggccgag aagcaggcga cgccggtcga
     4981 ggtgctcttc cccgactggg agccgatcgg cgagacgctc atcgcgaccc tccggaagga
     5041 ctccgtcaag accaagcgcg aggcgctcct cgagatctac aagcggatgc gccccggcga
     5101 cccgccgacc gacgagagcg cgaagaacct cttcgtcggg atgttcttcg acccgcgcaa
     5161 gtacgacttc tcccgcgtgg gccgctacaa gttcaacatc cggatgggcc tctcgacctc
     5221 gctcgagcag aagacgctct cgtccgacga cttctacgtc gtcatcgacg cgctcctgaa
     5281 gctccggaag gacatcggca agaccgacga catcgacaac ctcggcaacc gccgggtccg
     5341 ctgcgtcggc gagctcctcg agaaccagtt ccgcatcggc ctcgtccgga tggagcgcgc
     5401 gatcaaggag aagatgtccg tccaccagga catcgactcc gcgatgcccc acgacctgat
     5461 caactccaag ccggtcatcg cggcgatcaa ggagttcttc gggtcgtcgc agctctcgca
     5521 gttcatggac cagacgaacc cgctctcgga ggtgacgcac aagcgccgcc tctcggccct
     5581 cgggccgggc ggcctgacgc gcgagcgcgc cggcttcgag gtccgcgacg tccacgccac
     5641 gcactacggc cgcatctgcc cgatcgagac gccggaaggc ccgaacatcg gcctcatctc
     5701 ctcgctctcg acctacgcgc ggatctccga gtacggcttc atcgagtcgc cctacaagaa
     5761 ggtccgggac gggcgggtcc tcgagcactt cgagatcttc acggtcggcg actcgggctg
     5821 gaagaagggc gcgatcgtcg aggcctccga gctcgaggcg atgaacgcga agctgaagaa
     5881 gggcggcaag aggctcgccg tcggccggcc caccgccttc tacctcccgg cgtgggagga
     5941 ggaggactac gcgatcgcgc aggcgaacga gcctctcgag gccgacggcc gcttcgcgaa
     6001 cgaccgggtc caggtgacgc tccagggaga gttcagcgcg aagccccggg agacggtcca
     6061 cttcatggac gtctccccga agcagctcgt ctccgtcgcc gccgcgctca tccccttcct
     6121 cgagcacgac gacgcgaacc gggccctcat gggatcgaac atgcagcggc agtccgtgcc
     6181 gctcctccgc accgagccgc cgatcgtcgg caccggcctc gagggggtcg tcgcccgcga
     6241 ctccggcgcc gtcgtcctct gcaagcgcga cggcatcgtc gacacggtcg acgcgcgccg
     6301 gatcatcgtg agggtcgagg ggatcgggcc cgacggcaag ccccgggagt tcggggcgga
     6361 catctacccg ctcctgaagt tccggcggtc gaaccagaac acctgcatca accagaagcc
     6421 gatcgtcaag gaggggcaga gggtgagggc cggcgaggtc ctcgcggacg gcccgtgcac
     6481 ctcggggggc gagctcgccc tcggccggaa catcctcgtg gccttcatgc cgtggcgcgg
     6541 ctacaacttc gaggacgcga tcctgatctc cgagaagatg gtgaaggagg actactacac
     6601 ctccatccac atcgaggagt tcgagatcga ggcccgcgag accaagctcg gccccgagga
     6661 gatcaccaag gacatcccga acgtctccga gcacgcgctg agggacctcg acgagtcggg
     6721 gatcatccgg atcggcgcga acgtgaagcc gggcgacatc ctcgtcggca aggtcacgcc
     6781 gaagggcgag acgcagctca cgcccgagga gaagctcctc cgggcgatct tcggcgagaa
     6841 ggccggcgac gtccgcgacg cctcgctcaa gtgcccgccg gggatcgaag gcgtcgtcgt
     6901 cgacgtgaag atcttcacgc gcaaggacga cgagaagcag aaggacgtcc gggccaagca
     6961 gatcctcgac gaggagatcg gcttcctcaa caaggacgcc gaggacgaga tccggatcgt
     7021 ccggacggag gccaaggaga agatcgagcg cctcctcgag gggcgcacgg tcctcgagga
     7081 cgtcgtcgac ccgcagggga acgtcctcgt ggccgcgaac cacaagctca cggtcgagaa
     7141 gatcgggatg atggggccgc ggctcctcaa gagcctgaag ctccgcgacg aggagatcaa
     7201 ggacaccgtc cggctcgtcc tccagcgcgc cgactcccag gtggacgtca tccgcaaggt
     7261 ggcggccgac cgcgtcacgc tcctctcgaa gggcgacgag ctcgcccccg gcgtcaacaa
     7321 gacggtcaag gtctacgtcg ccatgaagcg caagctccag gtcggcgaca agatggccgg
     7381 ccggcacggg aacaagggcg tcatcagccg gatcctcccc gaggaggaca tgccgtacct
     7441 gccggacggc acgccggtcc agatcgtcct gaacccgctc ggcgtcccgt cgcggatgaa
     7501 cgtcgggcag atcctcgaga cgcacctcgg ctgggccgcg caggcgctcg gcctgagcat
     7561 cgcctcgccc gtcttcgacg gggcctccga ggaggagatc cgggcgctcc tcgagaagtc
     7621 gaacctcccg cggagcggca agacggagct ctacgacggg atgacgggcg agaggttcga
     7681 gcagaaggtc acggtcggct acatctacat gctcaagctg tcccacctcg tggacgacaa
     7741 gatccacgcc cgctccatcg ggccctactc gctcatcacg cagcagcccc tgggcggcaa
     7801 ggcgcagttc ggcggccagc ggttcggcga gatggaggtc tgggccctcg aggcctacgg
     7861 ggcggcctac acgctccagg agctcctcac ggtcaagtcc gacgacgtcg acgggcgctc
     7921 ccgcgtctac gaggcgatcg tcaagggcga ggttcccgag gagccgggcc tgcccgagtc
     7981 gttcaacgtc ctcgtccgcg agctgcagtc gctctgcctc gacgtcgagc tcctgaaggt
     8041 ctgaggaggg agagatgacc atggacacct tcggcgcgtc ccccctctcg cccgcggtcc
     8101 cggccttccg cggcccgggc ccgcgggact tcaacgcgat caagatcgcc ctggcgagcc
     8161 ccgagaagat ccgctcctgg tccttcggcg aggtgacgaa gccggagacg atcaactacc
     8221 ggacgttcaa gccggagcgc gacggcctct tctgcgcgaa gatcttcggg ccgatcaccg
     8281 actgggaatg cctctgcggc aagtacaagc ggatgaagca ccggggcgtc gtctgcgaca
     8341 agtgcggcgt cgaggtgacg aagtcgaagg tccggcgcga gcggatgggg cacatcgagc
     8401 tcgcggcccc ggtctcgcac gtctggttct tcaagggcct cccgagccgg atcgggcacc
     8461 ttctcgacct gaccctccgc gacctcgagc ggatcctcta tttcgaggcc tacgtcgtcg
     8521 tcgacccggg cccgctcgcg gaggaggaga acgtcaacga gaaggtgctc atcacggagg
     8581 agcgctaccg ggacctccgg gccaagtacg gcgccgacgc gttcgtcgcg aagatgggcg
     8641 ccgaggcgat ccaggagctc ctccagcggg tcaacatcga cgacctcgcc gacgagctcc
     8701 gcctcatcat gaagacggag acctccgccc tgaagcggct gaaggccgcc aagcgcctca
     8761 aggtcgtcga cgccttccgc cgctcgggcc acaggccgga gtggatgatc ctgaacgtca
     8821 tcccggtcat cccgccggag ctccgcccgc tcgtgccgct cgacggcggg cgcttcgcga
     8881 cctcggacct gaacgacctc taccggcggg tcatcaaccg gaacaaccgg ctgaagaagc
     8941 tccttgagct ccgcgcgccc gaggtcatcg tccgcaacga gaagcggatg ctccaggagg
     9001 cggtcgacgc cctcttcgac aacggccgtc gcggccgcgt cctgaagggg acgaacaacc
     9061 gcccgctgaa gtcgctctcc gacaccctca aggggaagtc gggccgcttc cgccagaacc
     9121 tgctcggcaa gcgcgtcgac tactcgggcc ggtcggtcat cgtcgtcggc cccgagctga
     9181 agctgaacca gtgcggcctc ccgaagcgga tggccctcga gctcttcaag cccttcatct
     9241 accggcgcct cgagcagaag gggctcacct cgacgatcaa gatgtcgaag gagctcgtcg
     9301 aggcggcgcc gaacgaggtg tgggacgccc tcgaggaggt catcaaggag cacccggtcc
     9361 tcctgaaccg ggcgcccacg ctccaccgcc tcggcatcca ggcgttcgag ccggtcctca
     9421 tcgaaggcaa ggcgatcaag atccacccgc tcgtctgcgc ggccttcaac gcggacttcg
     9481 acggcgacca gatggccgtc cacgtgccgc tctcgccgaa ggcgcaggtc gaggcccagg
     9541 tcctcatgct ctcggcgcac aacatcctgt cgcccgcgag cggcaagccg ctcacggtgc
     9601 cctcgcagga cctcgtcctc ggcctctact acctgacccg ccgcaaggag ggccgcaagg
     9661 gcgagggccg ggtcttcgcg ggcttcgacg acgtcctcct cgcgcacggc gccggggagc
     9721 tcgacaccca cgccgggatc accgtccgct acaccggacg cctcatcgac ctcgagaacc
     9781 aggtgatcgg cgaccagcag gacatcgtcc acgccgagcc gatcgactgc cacgggaccc
     9841 ggatcgagac gacggtggga cgcgtcctga tgtgcgcgca cctcccgaag gacatcccgt
     9901 tcatcaacgg gacgctgaag aagaagggcc tgcaggacct cgtcaactac tgcttcgtga
     9961 acctcgggct ggagcggacg gtcgagatcc tcgacatcat caagcagctc ggcttcgcgg
    10021 ccgcgacgcg ggccgggatc tcgatcggcg tcgacgacat gcggatcccg tcgaagaaga
    10081 cgggcttcgt cgacaaggcg cgcaaggacg tcctcgaggt cgacaaccag cgggcccagg
    10141 gcgcgatcac cgacggcgag cgccacaaca agatcatcga catctggcac cgcgcgaccg
    10201 aggccgtctc cgaggccatg atgaaggaga tgaaggccga ggacgcggcc ccggggaacg
    10261 agttcaaccc gatcttcatg atggccgact ccggcgcccg aggctccaag gagcaggtgc
    10321 gccagctcgc cggcatgcgc ggccccatgt ccaagccctc gggcgaggtc atggagcagc
    10381 cgatcatctc gaacttccgc gaggggctct ccgtcctcga gtacttcatc tcgacgcacg
    10441 gagcccgcaa gggcctcgcg gacacggcgc tcaagacggc cgactccggc tacctcacgc
    10501 gccgcctcgt ggacgtcgcc caggacgtca tcatcaacga ggaggactgc gccacgctcg
    10561 acgggatcac cgtcacggcg atcgtcgagg ggggcgagat cctggagccc ctccgcgacc
    10621 gcatcgtcgg ccgcttcgtc caggaggacg tctacgaccc ggcgacggac gaggtcgtca
    10681 tcgccgccaa cgacctgatc accgaggaga tcgcaaccgc catccaggag gcgggcatcg
    10741 agaaggtgaa gatccgctcg gtcctgacat gcgagacccg ccgcggcgtc tgccggaagt
    10801 gctacggccg gaacctcgcc acgggccgga tggtcgacat cggcgaggcg gtcggcgtca
    10861 tcgccgcgca gtcgatcggc gagccgggca cccagctcac gatgcggacg ttccactacg
    10921 gcgggacggc gcgcgtctcg gagaccgccc gccaccaggc gaagaacccc ggcgtcgcgc
    10981 ggttcctcaa cgcgctcacg gtgcgcaaga gcgacggcac ggaggtcgtc gtcaatcgca
    11041 actccaagct cgtcatccag gacgcgaagg gccgggagaa ggagcgctac tccctcgcct
    11101 acggctccgt cctcaaggtg acctcgggcg aggaggtcaa gcccggccag ctcctcgtca
    11161 cctgggaccc cttcgcctcg ccgatcctct cggagatcga gggcaaggtc gtcttcaagg
    11221 acatcctcga gggggagaac ctccgcgagg agacggacaa ggtcacgggc ctctcgcaga
    11281 agatcgtcgt cgagacgacc gggacgaccg agaagcgggc gccccagctc gtcgtcctcg
    11341 gcgtggacgg caccgagcgc cggtacgccc ttccgatccc gtcgcacctc atggtcagcg
    11401 acggcgacac ggtcaccccg ggcgacgctc tcgccaagaa gccggtcgag aagaagagga
    11461 cgcgcgacat cgtcggcggc ctcccgcgcg tcgtcgagct cttcgaggcc cggcgtccga
    11521 aggagcccgc ggtcatcacc gaggtctccg gcgccgtccg tcacgccggg acggtcaagg
    11581 ggcagcagaa gctcgtcgtc gagtccgagg acgggcagaa gcgcgacatc ctcgtcccgc
    11641 gcggcgccca cgtcatggtc caggagggcg agcgcgtcga ggcgggggat cccctcaccg
    11701 agggcgaccc ctcgccgcac gacatcctcg cggtcaaggg cgagaaggag ctccagcggt
    11761 acatgaccga caagatccag gaggtctacc gctcccaggg cgtcggcatc aacgacaagc
    11821 acatcgaggt catcgtccgc cagatgatgc ggagcgtccg cgtcgaggag gtcggcgaca
    11881 ccgagttcct gatcgacgag caggtcgaca agttccggtt ccgcgaggag aacgaccgga
    11941 tcatcgccgc cggcggcaag ccggcgcagg gccgtccgct cctcctcggc atcacgaagg
    12001 cctcgctctc gaccgactcc ttcgtgtcgg ccgcctcctt ccaggagacg acccgcgtcc
    12061 tcacggaggc gtcgatcgcg ggccgggtgg acctcctgcg cggcctgaag gagaacgtca
    12121 tcgtcggccg gctcatcccg gccgggacgg ggatggactt ctaccggaac gtcgagctcg
    12181 aacgggagga gatcccgatc cagcccgacg agatcgagga gccgttctac gacgacgagc
    12241 cgccggtcgt ggcggcggac ctcgacgagg agatctgaaa tgccctgggg ggacccggac
    12301 cgccgggtcc ccccaaaccc cctccgcgcg ggaaggcagc tcgctgcgct cgcgctgagt
    12361 aacgggggac ccggaccgcc gggttccctt ccggccccga tggcgtccgg tcgacgtacc
    12421 gggttgacat acgggggact tacacgtagt ctctgccggt ttcgcccctc ggacagagcc
    12481 gattcggcct tccgagagag cgatccgaca tcgaaaattc cggggagaga gcggccccaa
    12541 gggccgaaaa gtcgtctctc cggcggggaa gaggaaccga ggaagtctga tgccgacgat
    12601 cagccagctc gttcgtaagg gccgcgaagc cgtccgttac aagacgaagt cgcccgccct
    12661 ccagtcctgc ccgcagaggc ggggggtctg cacccaggtc aagaccacga cccccaagaa
    12721 gccgaactcg gcgctccgaa agatcgcccg cgtgaggctc acgaacggca tcgaggtcac
    12781 gacctacatt cccggcatcg ggcacaacct ccaggagcac tccatcgtca tgattcgtgg
    12841 cggccgcgtg aaggaccttc ccggcgtccg ctaccgcatc atccgtggga cgctcgacgc
    12901 cgtcggcgtc gcgaaccgga agcagtcccg ctccaagtac ggcgcca
//
LOCUS       DAOTEZ010000072        12945 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3378_length_12945_cov_5.686424, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000072 DAOTEZ010000000
VERSION     DAOTEZ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12945
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3378_length_12945_cov_5.686424"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="P5164_05640"
     CDS             complement(<1..259)
                     /locus_tag="P5164_05640"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY43412.1"
                     /translation="MPDALPRRKAEADIPRPDRVEKGERPEAVVKRLSEVEEEEIRWL
                     WPGRLAFGKFNILDGDPGEGKSLFTVDLAARVTTGRPLPGFT"
     gene            complement(252..1268)
                     /locus_tag="P5164_05645"
     CDS             complement(252..1268)
                     /locus_tag="P5164_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43413.1"
                     /translation="MTRIDLDALRSSSAARMGDVLAGIGAELARWAKPDRGRARCPFG
                     CGATGFPVSYADGRARCHYEAKSWDAIALVAERYGLEFRAAAERLSDILGGDFAEAPR
                     RRSEPVRQLRQAEDVSRLWEALPARDERGEAYLAGRGLWHGPFPDVVRFNVPGATKDE
                     DLARRARQGYRVAFGARRPDGKVANISLRYVGDGPPPDEKKTVSLAGGSTKSAAIMRP
                     EAAQLAADFAGDTVLLCEGGPDFLAWTHSLDVAALEGEEPPTWVLGCIGAGIAESVVS
                     TFAPLLAGRWVRLALDADKAGEDGAARAAAAAWRAGASRVTRITLDGAKDAAEVAEQE
                     ARNA"
     gene            complement(1265..1468)
                     /locus_tag="P5164_05650"
     CDS             complement(1265..1468)
                     /locus_tag="P5164_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43414.1"
                     /translation="MTAPALARVVPHPRMTGSKSDRVAVAEAVLVAEARRLAEVLGLD
                     DVAGLLGGASGLAASETWEEARR"
     gene            complement(1465..1713)
                     /locus_tag="P5164_05655"
     CDS             complement(1465..1713)
                     /locus_tag="P5164_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43415.1"
                     /translation="MNAPVPWQRLSDVLLEVDADPTLREVLEAGRNVEAEPGDGAAAF
                     LPDGETLLLLADGDGLLVRRHAPADEVALFLAGVREAA"
     gene            complement(1710..2114)
                     /locus_tag="P5164_05660"
     CDS             complement(1710..2114)
                     /locus_tag="P5164_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43416.1"
                     /translation="MPHKYAKSAGANGARRQGKRDNWQGKDSTAAPGRNRPVSSTSPE
                     RKSARLGAPARKPAERIAWERELSRFLGDSPCSAEHERPGGVNLYRAAGRIERLAEAI
                     ARELDVHPTSALEFALMSLVMNDPAVWPKETR"
     gene            complement(2127..2336)
                     /locus_tag="P5164_05665"
     CDS             complement(2127..2336)
                     /locus_tag="P5164_05665"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY43417.1"
                     /translation="MSTNPRTRTRFRETLLVRLTDAAATLGLSEAAIRNEIKAGRLEA
                     RKAGRAVLVPADELRRWAGDLPPAA"
     gene            complement(2430..3044)
                     /locus_tag="P5164_05670"
     CDS             complement(2430..3044)
                     /locus_tag="P5164_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43418.1"
                     /translation="MATTKKAWPTKDWDLEAGASLRRAWGRDGKTMMALLLGGEGEVS
                     TWLVSQGRESDSPLMPEDLVPLLRATRNPPISVRFWWALSLARSSQAIAFSGVSPTGV
                     VTVRSDRPISPKLPSIVDVRREAAPDTFAQVMKLMSAVKVLMPPHLRAVAIARTLSAG
                     KAHPHSEEAGKTMIRRERRKAERSGVMLAPLKRGKTGRPKKSGQ"
     gene            complement(3150..3569)
                     /locus_tag="P5164_05675"
     CDS             complement(3150..3569)
                     /locus_tag="P5164_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43419.1"
                     /translation="MSAFLVSHATVDVILTTLQLRDGETLARLFASFTAPEDPRDALD
                     SLGRQVLALNLEALRARYGGNEEDDAETAAGYTFNARPESPGYVVKQLDCLMYQCAEG
                     DVPSRPLYAAVEAMRRALVDRVLEDVPSYARAPWGVP"
     gene            complement(3566..4156)
                     /locus_tag="P5164_05680"
     CDS             complement(3566..4156)
                     /locus_tag="P5164_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43420.1"
                     /translation="MSEVSIPETLASLAANVAAQHDEMTVAMEEERAAEERLLGQLVE
                     AVRPALRALSSRLLASERTFWPDNVSTATEKDYHEDRGVRLAGSGPERDHPRANRGAI
                     EGLDLVLLDDGTFARLDWSGHWSRWQGEEDLEESTLARVSLRDVVDGWDVDQLAAALH
                     ERLEAQMTGRAPKRTASARQRAEKLAAVSSLLKGRA"
     gene            complement(4185..4346)
                     /locus_tag="P5164_05685"
     CDS             complement(4185..4346)
                     /locus_tag="P5164_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43421.1"
                     /translation="MSALVLRRLPSGRWALVAVPDAQPVATERRPVPREEGRRLPFLA
                     TPALTETPS"
     gene            complement(4343..4570)
                     /locus_tag="P5164_05690"
     CDS             complement(4343..4570)
                     /locus_tag="P5164_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43422.1"
                     /translation="MSADLVTVAPGDVYSAHMGGNGDAGPVSATISVHAPTGRVVYQV
                     VSPGVMRLGPHVSAFRAKGYAVTPLASEAGR"
     gene            complement(4567..4764)
                     /locus_tag="P5164_05695"
     CDS             complement(4567..4764)
                     /locus_tag="P5164_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43423.1"
                     /translation="MAAFGGDGPLEERVSRLEGLFAYLGEGILRSLPDRLTTDALIEI
                     LGDRDDVASRRAADALFEAKR"
     gene            5082..5924
                     /locus_tag="P5164_05700"
     CDS             5082..5924
                     /locus_tag="P5164_05700"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRY43424.1"
                     /translation="MSRLTRLLSGAALLLAPLLLASCGPKASEIRLKETSLKVYGLDR
                     VVSVGGDVVDKKGEIVPGLNVAWESSDPKVATVDPQTGSVKTVSAGKATITARLGKPP
                     LEARVALEVVDVASVHVSPQRMTLAGPAGTTFPLVLEAKASTGALLSMAADWTSSDPK
                     VATVDASGVVTSVAEGKATVKAAIGDVSGATDVVVVLRPIESLEATPLNIPLKIGEAL
                     DVTLVARDALGAPIPDAAATWTSSDPSVATCSGGRVLGVGPGSATIRAVCGAKTAEVA
                     VIVF"
     gene            5921..6796
                     /gene="amrB"
                     /locus_tag="P5164_05705"
     CDS             5921..6796
                     /gene="amrB"
                     /locus_tag="P5164_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HRY43425.1"
                     /translation="MTEAVRLPAVAGLFYEGDARALRADVGRYLGAAAFPPRPASAWR
                     ALLLPHAGHVYSGAVCGAGVGAVELPATVLLLGPNHHGAGAGVALSEAAAWRTPFGDV
                     PLAGGLADELRKTSPAIRTDEAAHRREHALEVILPFLQAARPGLSIACLTLGEPDLSV
                     CLEVGRAVAEAVTRVEARGERVGIVVSSDLNHYLPRERNREKDDRAIDALLLGDPSEL
                     FTRVRVRERISMCGILPATALLAALRDLPPTRPRLVARGDSGDAFGDTSRVVGYAALL
                     WETDTGTNGSDRKEC"
     gene            6796..8280
                     /locus_tag="P5164_05710"
     CDS             6796..8280
                     /locus_tag="P5164_05710"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRY43426.1"
                     /translation="MRVANFSAEVSPFAKTGGLGDVVSSLPKALAATGHDVDVWVPFF
                     LEAAEWFRRRGTWPEQEIEPFDVSILGATYRIGVLRSTLPGSSVPVHFVAHDPLFHRP
                     GGLYAKNDAGVDDGIWRFSLFVRAAIEALRRKGDRPEVIHAHDWHPGLAPMLGAWSSW
                     RDPWFDEVASVLTIHNVGYQGIYSPDQVAALGLPPETWSGGLLEHSGALNMLKGAIVA
                     ADMVTTVSPTYAWEIRTPEGGAGLDGVLRLKAARLTGILNGIDRQEWNPSVDPHLASR
                     YSAEDLRGKLDCRADLCRLAGFEPGDPSMIVGSVGRLVPQKGYDLLLEAAPELIRRGV
                     RLVLLGSGEPALEGSMRMLEAHHPGRFKGFVGYDDAFSHKIQAGSDALVMPSRYEPCG
                     LTQMYALAYGTVPIVRRTGGLADSVIGLHGENLEWATGFHFDDPSPHAVAAEVLHAQS
                     VFFRREAWSRLVQNGMKADFSWGRSANDYELVYRRAREVRGLPW"
     gene            complement(8290..9108)
                     /locus_tag="P5164_05715"
     CDS             complement(8290..9108)
                     /locus_tag="P5164_05715"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRY43427.1"
                     /translation="MEGSRALDTAVEAARRAGDLLLGFFGSLEPSEIEEKGRNDVVSR
                     ADRESEALVKELLLGAFPGDLFVGEEGGVKGRDGDAPAWIVDPLDGTANFVRGFPHWA
                     VSIGRTAGGAGGELVLGVVWDPVKNDLFVAEKGAGAYRNGRRVRVTERSGLAGAALAT
                     GFPFRVQARIDTYLRVFKAVFLEAQSIRRAGSAALDLAYVSCGIFDGFFELGLSPWDS
                     AAGAVLVREAGGVVTDFDGRDAWRERGNLLAAGPGVHAALAKLVAGLGVRDEQL"
     gene            complement(9108..9512)
                     /locus_tag="P5164_05720"
     CDS             complement(9108..9512)
                     /locus_tag="P5164_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY43428.1"
                     /translation="MPSSAPPAPPKRVVLVDDSPAYAASWRAIFAERYGDRVVFEAYQ
                     DPVAALEKLGPGVDLLLVDLEMPVLDGRSVARFAKERGVSEKKIVIVSGHDADELHLL
                     FPQGACLAVINKTEAQQQSAFLMILDSIVLRH"
     gene            complement(9552..10859)
                     /locus_tag="P5164_05725"
     CDS             complement(9552..10859)
                     /locus_tag="P5164_05725"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRY43429.1"
                     /translation="MSDAPVLITGGRVLDPSRGLDATLDVLLEGGVVARVDERIAPPR
                     GAETVDASGLVVAPGFVDLHVHFREPGLEWKETIASGSAAAAAGGFTTVCCMPNTDPV
                     IDDAPTASFVQKRIRETAHVRVLPIGAVSKGQKGVTLAEIGLMKAEGIVAISDDGKPV
                     VNADLMRRALEYASMFDLPVVVHEEDPNLVGGGQMHEGWASTRLGLPGWPAVAESTMV
                     ARDVELAALTKARLHVAHVSVRDSLAAIRRARAAHAPVSCEVTPHHLTLTDEDLARSG
                     YDTRFKMNPPLREEADRQALVSALADGTIDCVATDHAPHHADEKAMEFAAAPFGVTGL
                     ETALPVLLDGLVAPRLLSLSRLVEAMSIAPARLFGLPYGTLADGAPADVVLFSTTRTT
                     TFSAFRSLSANSPWAGKTLRGRIERTFVGGREVYRAGAAPAAG"
     gene            complement(10935..11897)
                     /locus_tag="P5164_05730"
     CDS             complement(10935..11897)
                     /locus_tag="P5164_05730"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HRY43430.1"
                     /translation="MNRKPETPADPASFTKKDLLDIESLSKAEIQMILDTAESMAEIN
                     KRTIKKVPTLRGRLVANLFYENSTRTRFSFEIAERRLTADSISFAAATSSASKGETLL
                     DTCRNLEAMAPDAIVIRHQASGAPHFLARSLKCAVINAGDGTHAHPTQALLDAFTIRK
                     AKGKLDGLRVAICGDVRFSRVARSNLALLPKMGAEVTLAGPRTLLPPEIERTGARVTT
                     RIEEAVDGADVVMMLRVQKERGGGLGFPSDREYFELFGLTPERRKLARKDAIVMHPGP
                     MNRGVEIDSAVADGPGSVILDQVEAGVAVRMAVLYLLIGVSGDE"
     gene            complement(12035..12586)
                     /gene="pyrR"
                     /locus_tag="P5164_05735"
                     /pseudo
     CDS             complement(12035..12586)
                     /gene="pyrR"
                     /locus_tag="P5164_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553637.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
     gene            12681..>12945
                     /locus_tag="P5164_05740"
     CDS             12681..>12945
                     /locus_tag="P5164_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HRY43431.1"
                     /translation="MNVSDFDFDLPPESIAQRPAPRGTSRLMTLDRSSGAIGHANVAD
                     LPSLLLPGDLLVVNDTRVVPARLFGRDADGRRTEFLLVEKTEED"
BASE COUNT         1745 a         4615 c         4700 g         1885 t
ORIGIN      
        1 acgtgaagcc gggcagcggc cggcccgtcg tcacccgtgc cgcgaggtcg accgtgaaga
       61 ggctcttccc ttcgcccggg tcgccgtcga ggatgttgaa cttgccgaag gcgaggcggc
      121 ccggccagag ccagcgaatt tcctcctctt cgacctcgga gaggcgcttg acgacggcct
      181 ccgggcgctc gcccttctcg acgcgatccg ggcgcgggat gtcggcctcg gccttccggc
      241 gaggaagggc gtcaggcatt gcgggcctcc tgttcggcga cctcggccgc atccttcgcg
      301 ccgtcgaggg tgattcgtgt cacgcgggaa gcgccggccc tccaggcggc ggcggcggcg
      361 cgagctgcgc cgtcctcgcc ggccttgtcg gcgtcgagag cgagccggac ccagcggccc
      421 gcgaggagcg gcgcgaacgt cgagacgacg ctctccgcga tcccggcacc gatgcatccg
      481 aggacccagg tgggaggctc ttcgccctcg agggccgcga cgtcgaggga gtgagtccag
      541 gcgaggaagt caggcccgcc ctcgcacagg aggacggtgt cgcccgcgaa gtcggccgcg
      601 agctgcgccg cttcgggtcg catgatcgcg gcggacttcg tcgagccgcc cgcgagtgag
      661 accgtcttct tctcgtccgg cggcgggccg tcgccgacgt aacgaaggga gatgttcgcg
      721 accttcccat ccggccggcg ggcgccgaag gcgacgcggt atccctgccg ggcgcgacgg
      781 gcgaggtcct cgtccttggt ggcgcccggg acgttgaagc ggacgacgtc ggggaacggc
      841 ccgtgccaga ggccgcggcc ggcgagatac gcctccccgc gctcgtcccg ggccggcagg
      901 gcctcccaga ggcgcgagac gtcctcggcc tgtcggagct gtcgcacggg ctctgagcgc
      961 ctcctaggcg cctctgcgaa gtcgcctccg aggatgtcgg agagccgctc ggcggcggcg
     1021 cggaactcga ggccgtaccg ttcggcgacg agggcgatag cgtcccagct cttcgcctca
     1081 tagtggcagc gggcgcggcc gtcggcgtag ctgaccggga agccggttgc gccgcatccg
     1141 aatgggcaac gagcgcggcc tcggtccggc tttgcccagc gggcaagctc ggctccgatc
     1201 ccggccagga cgtcgcccat cctggcggcc gaggaggagc ggagggcgtc gaggtcgatc
     1261 ctcgtcatct ccgggcctcc tcccaggtct ccgacgcggc gaggcccgag gcgccgccca
     1321 ggaggccggc gacgtcgtcc aggccgagga cctcggcgag ccgccgagcc tcggcgacga
     1381 ggacggcctc ggcgacggcg acgcggtcag acttcgagcc ggtcatgcgc gggtgaggga
     1441 cgacgcgagc gagagcgggc gcggtcaagc ggcctccctc acgcccgcga ggaagagggc
     1501 gacctcgtcg gcgggagcgt gccggcggac gaggaggccg tctccgtcgg cgaggaggag
     1561 gagcgtctct ccgtccggca ggaacgccgc ggctccgtcg ccgggctccg cctcgacgtt
     1621 ccggcccgcc tcgaggacct cgcggagggt cgggtcggcg tcgacctcga ggaggacgtc
     1681 ggagagccgc tgccacggca cgggggcgtt caccgcgtct ccttcggcca gacggccggg
     1741 tcgttcatga cgagggacat gagggcgaat tcgagagccg aggtcggatg aacgtccagt
     1801 tcccgcgcga tcgcctcggc gagccgctcg attcggcccg ccgcgcggta gaggttcacg
     1861 cctcccggcc gctcatgctc ggccgaacag ggcgagtcgc cgaggaagcg ggagagttca
     1921 cgctcccagg cgatccgctc cgccggcttc cgggccggcg ctcccaggcg agcggactta
     1981 cgctccggag acgtgctact gaccggacgg ttgcgaccgg gcgccgcggt gctatccttg
     2041 ccctgccagt tgtcccgttt gccctgtcgt cgagcgccgt ttgccccggc gctcttcgcg
     2101 tatttgtgcg gcatcacgtt ctcctttcac gcggccggcg gaaggtcgcc cgcccatcgc
     2161 cgcagctcgt cggccgggac gagcacggca cggccggcct tcctcgcctc gaggcggccc
     2221 gccttgatct cgtttcggat tgccgcctcc gaaagcccca gcgtcgccgc ggcgtcggtc
     2281 agccggacga gcagcgtctc gcggaagcgg gttcgggttc gcgggttcgt ggacatcggt
     2341 tcgcctcctt ctctcggtcc gcgtgatgcg gacgagcgca gggtcggcga acgtgggatg
     2401 cgaaaaaagc gacgtttccg ggggggtgtc tactgaccgg atttcttcgg gcgcccggtc
     2461 ttccctctct tgagcggcgc gagcatcacg ccggaacgct cggccttccg gcgttcgcgg
     2521 cggatcatcg tcttccccgc ctcctcggaa tgcgggtgag ccttgccggc ggagagcgtt
     2581 cgcgcgatag ctacggcccg aaggtgcggc ggcatgagga ccttcacggc gctcatgagc
     2641 ttcatgactt gtgcgaacgt gtccggggcc gcctcgcgcc tcacgtcgac gatgctcggg
     2701 agcttcggcg agatcggtcg atccgatcgc accgtaacaa cccctgtcgg cgagacgccg
     2761 gaaaaggcga tggcctgcga tgatcgcgcg agcgagagag cccaccagaa gcgaacgctg
     2821 atcggcgggt tccgcgttgc cctcagaagc gggacgagat cctccggcat caggggcgag
     2881 tcgctctctc ggccctgcga cacgagccaa gtggacactt cgccttctcc cccgaggagg
     2941 agcgccatca tcgtcttccc gtcgcgcccc caggctcgcc ggaggctcgc tccggcttcg
     3001 aggtcccaat ccttcgtcgg ccaggccttc ttcgtcgtcg ccatgtcgcc tccacgcctc
     3061 ccgggaaagg ccccggcggg aagcgcgagg cgtgcgcttg tcggcccggg gatcagccgg
     3121 gcctatcccg ccgagggaat cgtaaggggt caaggcactc cccagggcgc ccgggcgtag
     3181 ctcgggacgt cctcgaggac gcggtcgacg agagcccgcc gcatcgcctc gacggccgcg
     3241 taaagcgggc gcgacggcac gtcgccctcc gcacactggt acatcaggca gtcgagctgc
     3301 ttgacgacgt agccgggcga ctcgggccgg gcgttgaacg tgtagccggc cgccgtctcg
     3361 gcgtcgtcct cttcgttgcc gccgtaccgg gcgcggagcg cctcgaggtt gagcgcgagg
     3421 acctggcggc cgagggagtc gagcgcgtcg cgcgggtcct ccggcgccgt gaaggacgcg
     3481 aagagccggg cgagcgtctc gccgtcgcgg agctgaaggg tcgtcaggat gacgtcgacc
     3541 gtcgcgtgag agacgaggaa ggcgctcatg cccggccctt gaggagagac gagaccgcgg
     3601 cgagcttctc ggcccgctgc cgggccgacg cggtccgctt cggagcacgg ccggtcatct
     3661 gcgcttcgag ccgctcgtga agggccgcgg cgagctggtc gacgtcccat ccgtcgacga
     3721 cgtcgcggag ggaaacgcgc gcgagcgtgg actcctcgag gtcctcctcg ccctgccagc
     3781 gggaccagtg gccggaccag tcgagacggg cgaacgtgcc gtcgtcgagg aggaccaggt
     3841 cgaggccctc gatcgcgcca cggttcgcgc gcggatggtc ccgctccggc ccggagccgg
     3901 cgagccgcac gccgcggtct tcgtggtagt ccttctcggt cgcggtgctg acgttgtccg
     3961 gccagaaggt ccgctccgac gccaggagcc gcgacgagag cgcccggagc gccgggcgga
     4021 ccgcctcgac gagctgcccg aggaggcgct cctcggcggc gcgctcctct tccatggcga
     4081 ccgtcatctc gtcgtgctgc gcggcgacgt tcgcggcgag ggaggcgagg gtttccggaa
     4141 tgctgacttc gctcatctca gagtcctcct ctgtccctcg ctcttcacga gggcgtctcg
     4201 gtcagggcag gtgtcgcgag gaacgggagc cgacggcctt cctcgcgcgg gacgggccgg
     4261 cgctccgtcg cgaccggctg agcgtccggg accgcgacga gcgcccatcg gccggacggg
     4321 aggcggcgga ggacgagcgc gctcatcggc cggcctcgct cgccaagggc gtgacggcgt
     4381 agcccttcgc gcggaacgcg gagacgtgcg gcccgagccg catcacgccg ggcgagacga
     4441 cctgatagac gacccggccc gtcggcgcgt gaacggagat cgtcgcggag acggggccgg
     4501 catccccgtt cccgcccatg tgcgcggagt agacgtcgcc gggcgcgacg gtgacgaggt
     4561 cggcgctcat cgcttcgcct cgaagagggc gtcagcggcc cggcgcgagg cgacgtcgtc
     4621 ccgatcgccg agaatctcga tgagagcgtc cgtcgtgagc cggtccggga gggagcgaag
     4681 gatgccctcg ccgaggtacg cgaagagccc ctcgaggcgg gagacgcgct cctcgagtgg
     4741 accatctccg ccgaaggccg ccaagttccc ggggacagat ttcccggcgg tcttggcggt
     4801 cttggcggtc ttcgagggcg ccgacggcgg gtagactcga ctcggccgcc cttcagggcg
     4861 tgccgctcgg agcccggcgc cgatcgtctt tgcgggagac ggcgtcgggt tcttcttcgt
     4921 tcgcataggt cctcctcacc gctccggcgg agcggctctc aagtggcgcc gggtggcgcc
     4981 gttcgttctt tcggtgcgat tcctgcgcgt catgacggga ccgaaactcg ctttctgcgg
     5041 cctatgcgga cggctcgcgg atggcctaag atttcggggc aatgagccgt ctcacccgcc
     5101 tcctctccgg cgcggcgctc ctcctcgccc ccctcctcct cgcctcctgc gggccgaagg
     5161 cctccgagat ccgcctcaag gagacgagcc tgaaggtcta cggcctcgac cgggtcgtct
     5221 ccgtcggcgg cgacgtcgtc gacaagaagg gggagatcgt ccccggcctg aacgtcgcct
     5281 gggagtcctc cgacccgaag gtcgccaccg tcgacccgca gaccggctcc gtgaagacgg
     5341 tctcggccgg caaggcgacc atcaccgcgc ggctcggcaa gccgcccctc gaggcacggg
     5401 tggcgctcga ggtcgtcgac gtcgcgtcgg tgcacgtctc tccccagcgg atgacgctcg
     5461 cgggccccgc cgggacgacc ttcccgctcg tcctcgaggc aaaggccagc acgggcgcgc
     5521 tcctctcgat ggcggcggat tggacctctt ccgacccgaa ggtggcgacg gtcgacgcgt
     5581 ccggcgtcgt gacctccgtc gccgaaggga aggcgacggt gaaggccgcg atcggcgacg
     5641 tctccggcgc gaccgacgtc gtcgtcgtcc tccggccgat cgagtccctg gaggcgactc
     5701 ccctgaacat cccgctgaag atcggcgagg cgctcgacgt caccctcgtc gcccgcgacg
     5761 cgctgggggc gccgatcccc gacgccgccg ctacgtggac ctcgtccgac ccctcggtcg
     5821 ccacctgctc cggcggaagg gtcctcggtg tcggccccgg ctccgccacc attcgtgcgg
     5881 tctgcggcgc gaagaccgca gaggtcgcgg tgatcgtgtt ttgaccgaag ccgtccgcct
     5941 ccccgcggtc gccggactct tctacgaggg cgacgcgcgg gcgctgcggg cggacgtcgg
     6001 gcggtacctg ggagccgcgg ccttcccgcc ccggcccgcc tcggcctggc gggcgctcct
     6061 gctgccgcat gccggccacg tctactccgg ggcggtctgc ggcgcgggag tcggcgccgt
     6121 cgagctcccc gcgacggtcc tcctcctcgg accgaaccac cacggagcgg gagcaggcgt
     6181 cgccctctcc gaggcggccg cctggcgaac gcctttcggc gacgtccccc tcgcgggagg
     6241 cctcgcggat gagctgcgca agacgtcccc cgcgatccgg acggacgagg cggcccaccg
     6301 ccgcgagcac gcgctcgagg tgatcctccc gtttcttcag gccgcgcggc ccggcctttc
     6361 catcgcctgt ctcaccctcg gcgagccgga cctctccgtc tgcctcgagg tcggccgggc
     6421 cgtcgcggag gccgtgacgc gcgtcgaggc gcgaggcgag cgcgtcggga tcgtcgtctc
     6481 atccgacctc aaccactacc tgccccgcga gcgaaaccgc gagaaggacg accgcgcgat
     6541 cgacgcgctc ctcctcggcg acccgtcgga gctttttacg cgggtccggg tacgggagcg
     6601 gatctccatg tgcggcatcc tgccggcgac ggccctgctc gcggccctcc gggacctgcc
     6661 ccccacccga ccgcgcctcg tggcgcgagg cgactcgggc gacgccttcg gcgacacgag
     6721 ccgcgtcgtc ggctacgccg ccctcctctg ggaaaccgac acgggaacga acggatcgga
     6781 taggaaggag tgctgatgcg cgttgcgaac ttctcggccg aggtctcgcc tttcgccaag
     6841 acgggcggcc tcggcgacgt cgtctcttcc ctccccaagg ccctcgccgc gaccggccac
     6901 gacgtggacg tctgggtgcc gttcttcctc gaggccgcgg agtggttccg ccggcgcgga
     6961 acgtggccgg agcaggagat cgagccgttc gacgtctcga tcctcggagc gacctacagg
     7021 atcggcgtgc tccggtcgac gctccccggc agcagcgtcc cggtccactt cgtggcgcac
     7081 gacccgctct tccaccgacc cggcgggctc tacgcgaaga acgacgcggg cgtcgacgac
     7141 gggatctggc gcttcagcct cttcgtccgc gcggcgatcg aggcgcttcg gcggaaggga
     7201 gaccggcccg aggtgatcca cgcgcacgac tggcatcccg ggctcgcgcc gatgctcggc
     7261 gcctggagct cctggcggga cccgtggttc gacgaggtcg cgtcggtcct gacgatccac
     7321 aacgtcggct accaggggat ctacagcccc gaccaggtcg ccgccctcgg ccttccgccc
     7381 gagacctgga gcggaggcct cctcgagcat tccggcgcgc tgaacatgct gaagggggcg
     7441 atcgtcgcgg ccgacatggt gacgacggtc tccccgacct acgcctggga gatccggacg
     7501 ccggaagggg gcgcgggcct cgacggtgtc ctccgcctga aggcggcccg cctgaccggg
     7561 atcctgaacg gcatcgaccg ccaggagtgg aacccgtccg tcgacccgca cctggcctcg
     7621 cgctactcgg ccgaggacct gcgcgggaag ctcgactgcc gcgccgacct ctgccgcctc
     7681 gcgggattcg agccgggcga cccgtcgatg atcgtcggct ccgtaggccg cctcgtcccg
     7741 cagaagggtt acgacctcct cctcgaggcc gcccccgagc tgatccggcg cggcgtgcgg
     7801 ctcgtcctcc tcggctcggg agagccggcg ctcgaaggct cgatgaggat gctcgaggcg
     7861 caccacccgg gccggttcaa gggcttcgtc gggtacgacg acgccttctc gcacaagatc
     7921 caggccggga gcgacgcgct cgtcatgccg tcgcgctacg agccgtgcgg cctgacgcag
     7981 atgtacgccc tcgcctacgg caccgtgccg atcgtgagga ggaccggcgg gctcgccgac
     8041 tccgtgatcg gccttcacgg cgagaacctc gagtgggcga ccggcttcca cttcgacgac
     8101 ccttccccgc atgccgtcgc cgccgaggtg ctccacgcgc agagcgtctt cttccgtcgt
     8161 gaggcgtggt cgcggctcgt tcagaacggt atgaaggccg acttctcctg gggccgatcc
     8221 gcgaacgact acgagctcgt ctaccggcgg gcccgggagg tccgcgggct cccctggtag
     8281 cgggcccggc tagagctgct cgtcccggac cccgaggccc gcgacgagct tcgcgagagc
     8341 ggcgtggacg ccgggtccgg cggcgaggag gtttccccgc tcgcgccatg cgtccctgcc
     8401 gtcgaagtcg gtgacgacgc cgccggcctc cctcacgagg acggcgcccg ccgcggaatc
     8461 ccacggcgag agccccagct cgaagaagcc gtcgaagatc ccgcaggaga cgtacgcgag
     8521 gtcgagggcc gcgctcccgg cccggcggat cgactgcgcc tcgaggaaga ccgccttgaa
     8581 gacgcggagg tacgtgtcga tacgcgcctg gacgcggaac gggaagccgg tcgcgagcgc
     8641 cgcccccgcg agcccgctcc gctccgtcac ccggacgcgg cggccgttgc ggtaggcgcc
     8701 cgcccccttc tccgcgacga agaggtcgtt cttcaccggg tcccagacga caccgaggac
     8761 gagctcgccg ccggcccctc ccgccgtgcg gccgatcgag accgcccagt gggggaagcc
     8821 gcggacgaag ttcgccgtgc cgtcgagagg gtcgacgatc cacgccggag cgtcgccgtc
     8881 ccgccccttc acgccgcctt cctcgccgac gaagaggtcc ccggggaagg cgccgaggag
     8941 gagctccttc acgagcgcct cgctctccct gtcggcgcgc gagacgacgt cgttccgccc
     9001 cttctcctcg atctccgacg gctcgagcga gccgaagaat ccgaggagga ggtcgccggc
     9061 gcggcgggcg gcctcgacgg ccgtgtccag ggcgcgggag ccctccatca gtgccggagg
     9121 acgatgctgt cgaggatcat caggaaggcg ctctgctgct gcgcctcggt cttgttgatg
     9181 accgcgaggc aggccccctg ggggaagagg aggtgcagct cgtcggcgtc gtgcccggag
     9241 acgatgacga tcttcttctc gctgacgccc cgctccttcg cgaagcgcgc gacggagcgc
     9301 ccgtccagga ccggcatctc gaggtcgacg aggaggaggt cgacgccggg gcccagcttc
     9361 tcgagcgcgg cgaccgggtc ctggtaggcc tcgaagacga cgcggtcgcc gtagcgctcc
     9421 gcgaagatcg cgcgccagga ggccgcgtac gcgggcgagt cgtcgacgag gacgacgcgc
     9481 ttcggcgggg cggggggggc ggacgacggc atggcggggg gctccgggag gggtgcgcga
     9541 accggccggc gtcagccggc ggccggcgcc gcaccggccc ggtagacctc gcgccctccg
     9601 acgaacgtcc tctcgatgcg gccccggagc gtcttcccgg cccagggcga gttcgcggag
     9661 agcgagcgga aggccgagaa cgtcgtcgtc cgtgtcgtgg agaagaggac gacgtcggcc
     9721 ggggccccgt ccgcgagcgt cccgtacggc aggccgaaga ggcgcgccgg ggcgatcgac
     9781 atcgcctcga cgaggcggga gagcgagagg agccgcgggg cgacgaggcc gtcgaggagg
     9841 acggggagcg ccgtctcgag gccggtgacc ccgaacggcg ccgcggcgaa ctccatcgcc
     9901 ttctcgtccg cgtggtgagg ggcgtggtcc gtcgcgacgc agtcgatcgt cccgtcggcg
     9961 agcgccgaga cgagagcctg gcggtccgcc tcctcgcgca gcggcgggtt catcttgaac
    10021 cgggtgtcgt agccggagcg ggcgaggtcc tcgtccgtca gcgtcaggtg gtgcggcgtc
    10081 acctcgcagc tgaccggggc gtgcgccgcg cgggcgcgcc ggatcgcggc gaggctgtcg
    10141 cgcacggaga cgtgagcgac gtggagccgc gccttcgtca gcgcggccag ctcgacgtcg
    10201 cgggccacca tcgtcgactc ggcgacggcg ggccagccgg ggaggccgag gcgcgtcgac
    10261 gcccacccct cgtgcatctg gccgcccccg acgaggttcg ggtcctcctc gtggacgacg
    10321 accggaaggt cgaacatcga ggcgtactcg agggcgcgcc gcatcaggtc ggcgttgacg
    10381 acgggcttcc cgtcgtcgga gatcgcgacg atcccctccg ccttcatgag gccgatctcg
    10441 gcgagcgtga cgcccttctg gcccttcgag acggcgccga tcgggaggac gcggacgtgt
    10501 gcggtctcgc ggatccgctt ctggacgaac gacgccgtcg gggcgtcgtc gatcacgggg
    10561 tccgtgttcg gcatgcagca gacggtcgtg aagccgcccg ccgcggccgc cgcggagccg
    10621 gacgcgatcg tctccttcca ctcgagcccg ggctcgcgga aatggacgtg gaggtcgacg
    10681 aagccggggg cgacgacgag gcccgaagcg tcgaccgtct cggcgccgcg aggaggcgcg
    10741 atccgctcgt ccacgcgcgc gacgacgccc ccctcgagga ggacgtcgag cgtggcatcg
    10801 aggccgcgcg acgggtcgag gacccgtccg ccggtgatca ggacgggggc gtcactcatc
    10861 gccgctcact ccgatcagga ggtgtcgagc gcgtgaatcg cgcgagcgag gagtgagcgg
    10921 ccgccttcgg cttcctattc atcgccgctc actccgatca ggaggtagag caccgccatc
    10981 ctcaccgcca cgcccgcctc gacctggtcg aggatgacgg agccgggacc gtcggccacg
    11041 gcggagtcga tctcgacgcc gcggttcatc gggccggggt gcatgacgat cgcgtccttg
    11101 cgcgcgagct tcctccgctc gggcgtcagg ccgaagagct cgaagtactc gcggtccgac
    11161 gggaagccga gcccgccgcc ccgctccttc tggacgcgga gcatcatcac gacgtcggcg
    11221 ccgtcgacgg cttcctcgat ccgggtcgtc acgcgcgccc ccgtccgctc gatctcgggc
    11281 ggcaggagcg tgcgcggtcc cgcgagcgtc acctcggccc ccatcttcgg gaggagcgcg
    11341 aggttcgagc gggcgacgcg gctgaagcgg acgtcgccgc agatcgcgac gcgcaggccg
    11401 tcgagcttcc ccttcgcttt ccggatcgtg aaggcgtcga ggagcgcctg cgtcgggtgc
    11461 gcatgcgtgc cgtcgcccgc gttgatgacc gcgcacttca ggctccgcgc gaggaagtgg
    11521 ggcgccccgg aggcctggtg gcggatgacg atggcgtcgg gcgccatcgc ctcgaggttc
    11581 cggcaggtgt cgaggagggt ctcccccttc gaggccgacg acgtcgcggc ggcgaaggag
    11641 atcgagtcgg ccgtgaggcg tcgctcggcg atctcgaagg agaaccgggt ccgcgtcgag
    11701 ttctcgtaga agaggttcgc gacgaggcgg ccgcgaaggg tcgggacctt cttgatcgtc
    11761 cgcttgttga tctccgccat cgactcggcc gtgtccagga tcatctggat ctcggccttc
    11821 gagagcgatt cgatgtcgag gagatccttc ttcgtgaagg aggcggggtc ggccggggtc
    11881 tcgggtttcc tgttcaccgc ttcccgcggg cgcgcccctt cggggcggcc ttccgggcgg
    11941 gcttcgccgc cgccgcgacg gctttcttca ccggcgtctt cctcgccccc ttcggcgccg
    12001 ccttgccctt gcgcgccgcc gcgggctccg tgtggcggtc aagcagccag acgtcgtcct
    12061 cgccgtcggt ctccttgaac gtgaccgaga cgacttcggt ggcggaggtg gagatcttct
    12121 tgccgacgag ctgcgcctcg atcgggagct cccgccggcc gcggtcgacg aggaccgcga
    12181 ggaggatccg gcgcgggcgg ccgaagtcga ggatcgcgtc gatcgcggcc cggaccgtcc
    12241 gccccgtgta gaggacgtcg tcgcagagga cgatcgtctt cgtctccacc gggaaggaga
    12301 tgtcggtgcg cttcaggacg ggcgacgagg cgacggtggt caggtcgtcg cggtagaggg
    12361 tgatgtcgag gaggccgacg gggacccggg ccccttcggt cttctcgatc tccagcgcga
    12421 gccgccgcgc gagcggcacg ccgcgcgtcc ggatgccgac gagagcgaac ccctcgacgc
    12481 cccccgcagc ttcgacgacc tcgcgcgcca tccgtgagat gacgcgggac atgccggccg
    12541 gatccatcag cctgagcttg cggtaggtgc cgttggcttc gcccatcgtt cgaaccggcg
    12601 ggacactaca cgttgaagac tcccggcgtc aacgccggtg tcaggctccg cgtcggccgc
    12661 cgaccggcca caatcccccc gtgaacgtct ccgacttcga cttcgacctc cccccggaat
    12721 cgatcgctca gcggcccgcg ccccgcggca cgtcgcggct catgacgctc gatcggtcca
    12781 gcggcgcgat cggacacgcc aacgtcgcgg accttccgtc gctcctcctc ccgggagacc
    12841 tcctcgtcgt gaacgacacg cgcgtcgtcc cggcgcgcct gttcggacgc gacgccgacg
    12901 ggcggcgcac ggagttcctc ctcgtcgaga agacggagga ggacg
//
LOCUS       DAOTEZ010000073        12819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3436_length_12819_cov_6.105766, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000073 DAOTEZ010000000
VERSION     DAOTEZ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12819
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3436_length_12819_cov_6.105766"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="P5164_05745"
     CDS             complement(<1..1045)
                     /locus_tag="P5164_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HRY43432.1"
                     /translation="MSDSFKTKTTLSAGGATVSYYSIETLAKEHPEVRTLPYSLKVLL
                     ENLLRHEDGRVVKKEDVLALATWDPKAEPDKEIAFHPARVLMQDFTGVPAVVDLAAMR
                     EAIVAMGGDAAKVNPLSPADLVIDHSVQVDNFASSLAFGKNVDLEYERNRERYEFLRW
                     GQNAFRNFRVVPPGTGICHQVNLEYLAKVVWTNEEGVAYPDTLVGTDSHTTMINGLGV
                     LGWGVGGIEAEAALLGQPVSMLIPEVVGMKLHGKLPEGATATDLVLTVTQMLRKKGVV
                     GKFVEFYGPGLAALPLTDRATIANMAPEYGATMGFFPVDGETVRYLEFTGRKALAPLV
                     EAYSKAQGLWRAED"
     gene            complement(1156..1428)
                     /locus_tag="P5164_05750"
     CDS             complement(1156..1428)
                     /locus_tag="P5164_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRY43433.1"
                     /translation="MPVYEYRCQACGKRTEDLVLAGDAADYVPECAHCGSHELSRLLS
                     SFAAHGGDKGAASFDAPCGMGACETGDMSQCGMGGCGMGGGDFDLN"
     gene            1530..2342
                     /locus_tag="P5164_05755"
     CDS             1530..2342
                     /locus_tag="P5164_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRY43434.1"
                     /translation="MKWVVFNQKGGVGKSTLVCNLAAVAADEGERSLVVDLDPQGCSS
                     HYLLGERAKAARPNLADFYERSLEIRLVDDGIRTFIHATPFEGLDVLPTQPVLETLHA
                     KLEGRHKIYRLRDALAPLDGYAHVFFDTPPALNFFTLSALIAAERVLIPFDCDDFSRR
                     ALYGLLDVVEEVRQDHNPKLAIAGIVVNAFQPRATLPQLLVDELKAEGLPVLTPYVSA
                     SVKVRESHQAATPLVHLAPSHKLSVELRQLSAALRKKARGAAKATASKAAAR"
     gene            2339..3541
                     /locus_tag="P5164_05760"
     CDS             2339..3541
                     /locus_tag="P5164_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRY43435.1"
                     /translation="MRGRGPAGRGGRGRGATWTRTAGLLAAFPLVLVAAGANPLLSAD
                     GARSAPSSPFAAGVDTLLLVGDAGAPAAGEPVLRALATEAAKEPERTLVVFLGDNLYP
                     NGLPPEGAASRAEGERRLAAQADAALGAGARVLFLPGNHDWDGMGKDGLEAVRRQERF
                     LVARSPRLLFAPGDGCPGPLLLPTPSGVTLAVLDTQWWLQHEGPRPEGAGSPCSARDA
                     EEAVAQLRSAAAAGPLALLTHHPLATGGPHGVRTGDWKAHLFPLRDAHPSLWVPLPGL
                     GTAWVAYRGGKGTGQDLASSRYRRLRSDLASALRDRPVPFQASGHDHSLQVLETPVPE
                     ARWVLISGSGIFPAGRVVSKLPSTRYATSRAGFLRVRLERGRAPHLDVLEVTPEGKAE
                     ERLSLDLR"
     gene            3613..4593
                     /locus_tag="P5164_05765"
     CDS             3613..4593
                     /locus_tag="P5164_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY43436.1"
                     /translation="MTAVLSAEGLAKTFRTPFSRRQVRALDGLDLQVASGEVFGLLGP
                     NGAGKTTTVKILLGLTHPTSGRAELFGLPVSDPASRRRVGYLPEGHRFPGYLTARQTL
                     SIFGRMSGVAEADLKARIPELLARVRLTDWADVKVKRFSKGMTQRLGLASALVHRPDL
                     LLLDEPTNGVDPVGRREIRDLLREEAARGAAILLNSHLLSEIEMTCDRVAVLRKGKAV
                     AAGRIADLTAQGFAYRFVGAPMDEELLGLFRDAGAAAERVNGHVKLTARDAEHLNTLV
                     DAARARGVLVTELTPLRSTLEDVFVDLVSAGDPGEAGGTVHGSQGEGRPR"
     gene            4590..5444
                     /locus_tag="P5164_05770"
     CDS             4590..5444
                     /locus_tag="P5164_05770"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY43437.1"
                     /translation="MKVLAANVEEVFREAAARWTLVAYGALSTLFIALFAAAVNLDVV
                     DGALAGAKLFGKQLEIGPRSVDLDKLVLGFESGFSGFLYVVATFLAVFATAHLVPRLQ
                     EKGTIDLYLSRPVGRVPLLLSRYVGGLLLAGLNVVYLIGAMGLLIWWKTGVVHPRFFL
                     GGAIILFTIATLMAFAFLVGVVTSSTAVSIMATFAVFFLAAILSGHRQIEAALATEWA
                     ASLVRGFYWVLPKTGQLAQATVDYVSGGELSRHKVEIDSLAVFGTTGLFGVVSLALSA
                     LLFRRKDF"
     assembly_gap    5456..5555
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5556..6263
                     /locus_tag="P5164_05775"
     CDS             <5556..6263
                     /locus_tag="P5164_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43438.1"
                     /translation="LVAFEGRFEPRRLAAAAAARGAEARTAAGATYFLLKDDRQTGKR
                     GGGAVAFVSDRLLVAGSEEGVAAALAARAAGGTGFDRGAGLGAWLERVNGNASGWLLV
                     DRTKLPEAKGSVTLSGNDGAVRNVIGAMNLVSAFTLEMTVKGDAIRLAGAGYSDDTET
                     RELLEDALRGILATMRIAAQEKPDVVSAIRKFKVASDRNAVTISGTLDGATIKALSAA
                     KAERENARAAGSKAATK"
     gene            6410..8434
                     /locus_tag="P5164_05780"
                     /pseudo
     CDS             6410..8434
                     /locus_tag="P5164_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
     gene            8432..9388
                     /locus_tag="P5164_05785"
     CDS             8432..9388
                     /locus_tag="P5164_05785"
                     /inference="COORDINATES: protein motif:HMM:NF018024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HRY43439.1"
                     /translation="MPDLPTSIPVPARPVHTYSIVARDPATGQLGVAVQSHWFSVGAL
                     VPWAEAGVGAVATQSFVDASYGPLGLSLLKAGRSAPDALAGLLAADAGRDVRQVAMID
                     AAGRVAAHTGARCVEAAGHYVGKDFSVQANMMRSASVWPAMAKAFEETRGDLAERMLA
                     ALEAAEAAGGDIRGKQSAALLVVSGNPTGRAWQDRLFDLRVEDSQAPLPELRRLVTLA
                     RAYALMNEGDLAVERKDDAAALKAYSAAQAIVPGNAEMTYWTAVSLVGMGKVDEALPL
                     FRKVFAIDRSWAEMTPRLPKSGLLPDDPALLARILAEAPGAR"
     gene            9424..11025
                     /locus_tag="P5164_05790"
     CDS             9424..11025
                     /locus_tag="P5164_05790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRY43440.1"
                     /translation="MNSAFAPTSGSATSGSEAKTGTERGPEASAPPRDDSGPGFRQLA
                     EVAPMGILVVGADEGLRYANPSAAAMLGRSVDDLLACRYGTLADPSCRDLLRQRIADR
                     IAGAPVPGTYETLFRRADGSSVAVELSGNLLGWHGEPCDLLFLSDVTVRRNALAALEE
                     EREAFRSVFENAGEAVFLTSPDGSISAANPAACRMLGRSEEEIRRLGRSGVLDLSDPR
                     VPAALEERARTGRFHGELRALRVSGEAFPAEITSDLFTDAHGKPRSATILRDVTERRR
                     AEEAFRRSEALVRSLNAELEQRVAERTEALAAANEELRGFSSSIAHDLTAPLRGINGL
                     AGRLLEDHGSNLGEEARGLLRRIVEASARGGRLIEALLDHARISVARLEREAVDVGDL
                     AAAILRRRAAEEPGRNVTWIAPSGLDARADRRLVGEILEHLLANAWKFTSPREAARIE
                     VGAATEGGETVFFVRDDGVGFEPAHASRLFELFHRAHAGTELSGLGVGLALAERAVRC
                     HGGWIRIDGAPGAGATVRFTLSPRR"
     gene            complement(11088..>12819)
                     /locus_tag="P5164_05795"
     CDS             complement(11088..>12819)
                     /locus_tag="P5164_05795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY43441.1"
                     /translation="EEKRLELTLDVDDGVPVALLGDVTRMKQVVMNLVGNAVKFTDAG
                     EVAVTVVSRPSGSPPPGTLVELEVAVRDTGIGIRPERAANLFESFRQGDSSTTRRFGG
                     TGLGLAISRRLAELMGGSIAFESEPGRGSTFRFVARLEAAMPPSGAIVPSRRASFAGR
                     RVLVVEERETSRALLAKTLSGRGLQVAASGSIAAAAERFRSGERFDLAVVDRDLPEGD
                     GLAAFTGLRSRFAPSLPFVLVVPMGAEAEEGVDGPVARVTRPVKPAVLIEAVAALLGG
                     SRPAQRRESAIRPRFDGAMGRRLPLRILLVEDNPTNQELALLMLGRLGYSADVATNGR
                     EAVEAVSSRVFDLVLMDVQMPELDGIEATRQIRAALGADGPRIAAMTAHALPGDREAC
                     LAAGMNDYLVKPIGISDLVAALERAATALSPLTEGDGDDVPVTPTPSPTPPLATPPGL
                     DGKAWARLHASLGARAVDLLPGIVRSFARESDGLLAAARSAADAERADELHRAVHTLR
                     STSASFGALDLSALCREAEARAKENETSTLEPLLDRIGAELARVRKALETVPGDGPMD
                     EPVPSPKSPD"
BASE COUNT         1748 a         4572 c         4613 g         1786 t
ORIGIN      
        1 catcctccgc gcgccagagg ccctgcgcct tgctgtacgc ctcgacgagc ggggcgagcg
       61 ccttccggcc cgtgaactcg aggtaccgca ccgtctcccc gtcgacgggg aagaagccca
      121 tcgtggcgcc gtactcgggc gccatgttcg cgatcgtggc ccggtcggtg agcgggaggg
      181 ccgcgagtcc cggtccgtag aactcgacga acttcccgac gacgcccttc ttccggagca
      241 tctgggtgac ggtgaggacg aggtccgtcg ccgtcgcccc ctcggggagc ttcccgtgga
      301 gcttcatccc gacgacctcg gggatgagca tcgagacggg ctggccgagg agggcggcct
      361 cggcctcgat cccgccgacg ccccagccga ggacgccgag gccgttgatc atcgtcgtgt
      421 gcgagtcggt cccgacgagc gtgtcggggt aggcgacccc ttcctcgttc gtccagacga
      481 ccttcgcgag gtactcgagg ttcacctggt ggcagatgcc ggtgccgggc gggacgacgc
      541 ggaagttcct gaacgcgttc tgcccccagc ggaggaactc gtagcgctcc cggttccgct
      601 cgtactcgag gtcgacgttc ttcccgaacg cgagcgagga ggcgaagttg tcgacctgga
      661 cggagtggtc gatgacgagg tcggcggggg aaaggggatt caccttcgcc gcgtcgcccc
      721 ccatcgccac gatggcctcg cgcatggcgg cgaggtcgac gacggccggg acgccggtga
      781 agtcctgcat caggacgcgc gcggggtgga aggcgatctc cttgtcgggc tccgccttcg
      841 ggtcccacgt cgcgagggcg aggacgtcct ccttcttcac gacgcgtccg tcctcgtggc
      901 ggaggaggtt ctcgaggagg accttcaggg agtaggggag cgtccggacc tccgggtgct
      961 ccttcgcgag cgtctcgatc gagtagtagg agacggtcgc gcctccggcg gagagggtgg
     1021 tcttcgtctt gaagctgtcg ctcatcgttc ctccgggcgg tcgggtggaa tacaggaccg
     1081 gcggggattc taatcgccgc agggcagcac ggccccgccg gccggaatcg gtgccggcag
     1141 cccggaggaa gggcctcagt tcaggtcgaa atcgccgccc cccatcccgc agccgcccat
     1201 cccgcactgg ctcatgtcgc cggtctcgca ggcgcccatg ccgcacgggg cgtcgaacga
     1261 ggccgcaccc ttgtcgccgc cgtgggccgc gaaggaggag aggaggcggg agagctcgtg
     1321 cgagccgcag tgggcgcact cggggacgta gtcggccgcg tcgccggcga ggacgaggtc
     1381 ctcggtcctc tttccgcagg cctggcaccg gtattcgtag acgggcatgt cggacctccc
     1441 gagaccgatt ctagggatcg gggctcgaag acggaagggg cgcgcgccgg gtgagatgat
     1501 gcccggatcg ccacgacggg aggggacgca tgaagtgggt cgtcttcaac cagaaggggg
     1561 gcgtcgggaa gtcgacgctc gtctgcaacc tcgccgccgt cgccgcggac gaaggggagc
     1621 gctccctcgt cgtcgacctc gacccgcagg gatgctcgtc gcactacctc ctcggcgagc
     1681 gggcgaaggc ggcccgcccg aacctcgccg acttctacga gcggagcctc gagatccgcc
     1741 tcgtcgacga cgggatccgc acgttcatcc acgcgacgcc tttcgaaggg ctcgacgtcc
     1801 tcccgacgca gccggtcctg gagacgctcc acgcgaagct cgagggacgg cacaagatct
     1861 accgcctccg cgacgccctc gccccgctcg acggctacgc ccacgtcttc ttcgacacgc
     1921 cgccggcgct caacttcttc accctctccg cgctcatcgc cgccgagcgg gtcctcatcc
     1981 cgttcgactg cgacgacttc tcccgccggg ctctctacgg gctcctcgac gtcgtcgagg
     2041 aggtgcggca ggaccacaac ccgaagctcg cgatcgccgg gatcgtcgtg aacgcgttcc
     2101 agccgcgcgc gacgctgccg cagctcctcg tggacgagct gaaggcggaa gggctgcccg
     2161 tcctgacgcc gtacgtctcg gcctcggtga aggtccgcga gtcgcaccag gcagcgacgc
     2221 cgctcgtcca cctcgccccg tcgcacaagc tctcggtcga gctccggcag ctttcggcgg
     2281 ctctccggaa gaaggcccgg ggcgcggcga aggcgacggc gtcgaaggca gcggcgaggt
     2341 gaggggccgc ggcccggccg gccgcggcgg ccgcgggcgc ggcgcgacgt ggacccggac
     2401 ggctggcctt ctcgccgcgt tccctctcgt cctcgtcgcc gccggagcaa acccgctcct
     2461 ctcggcggac ggagcgcggt ccgctccttc gagtcccttc gcggccgggg tcgacacgct
     2521 cctcctcgtc ggcgacgccg gcgccccggc ggccggcgag ccggtccttc gcgccctcgc
     2581 cacggaagcc gcgaaggagc ccgagcggac gctcgtcgtc ttcctcggcg acaaccttta
     2641 cccgaacggc ctgccgcccg agggggccgc ttcgcgcgcg gagggcgaac gccgcctcgc
     2701 ggcgcaggcg gacgccgcgc tcggcgccgg cgcccgcgtc ctcttcctcc ccgggaacca
     2761 cgactgggac gggatgggga aggacggcct cgaggccgtc cggaggcagg aacgcttcct
     2821 cgtcgctcgc tccccgcgcc tcctcttcgc cccgggagac ggctgccccg gcccgctcct
     2881 ccttccgacg ccttcggggg tcacgctcgc ggtcctcgac acgcagtggt ggctccagca
     2941 cgaggggccg aggcccgaag gggcgggctc gccctgctcg gcgagggacg cggaggaggc
     3001 ggtggcgcag ctgaggtcgg cggccgccgc gggcccgctc gcgctcctga cgcaccaccc
     3061 gctcgcgacg ggcggtccgc acggcgtgcg aacgggcgac tggaaggcgc atctcttccc
     3121 gctccgcgac gcgcacccgt cgctgtgggt tccgcttccg ggcctcggca cggcctgggt
     3181 cgcttaccgc ggcgggaagg gaaccgggca ggacctcgcc tcgagccgct accggcggct
     3241 caggtccgat ctcgcctcgg cgctccggga ccggcccgtc cccttccagg cctccggaca
     3301 cgaccactcg ctccaggtcc tcgagacgcc ggtgcccgag gcgcgctggg tcctgatctc
     3361 ggggtccggg atcttcccgg ccggacgcgt cgtctcgaag ctcccgtcga ctcgctacgc
     3421 gacgtcccga gcgggcttcc tcagggtccg gctcgagcgg ggcagggcgc ctcacctcga
     3481 cgtcctcgag gtgacgcccg aagggaaggc ggaagagcgt ctctccctcg acctccggta
     3541 gcgaatcggg acgtttcgtc tcacctctcc tcccgtggga acccggcggg cgacgacacc
     3601 gtagagggag ccatgaccgc cgttctctcc gccgaaggcc ttgcgaagac cttccggacc
     3661 ccgttctcga ggcggcaggt gagagccctg gacggtcttg accttcaggt cgcctctggc
     3721 gaggtcttcg gactcctcgg accgaacggg gcggggaaga cgacgaccgt caagatcctc
     3781 ctgggcctga ctcatcccac gtcggggagg gccgagctct tcggcctccc cgtctccgac
     3841 cccgcgagcc ggcggcgcgt cggctacctg ccggaggggc accgcttccc cggctacctg
     3901 accgcgcgtc agacgctctc gatcttcggg aggatgtcgg gcgtcgcgga ggccgacctg
     3961 aaggcccgga ttcccgagct cctcgcccgc gtccgactga cggactgggc ggacgtgaag
     4021 gtgaagcgct tctcgaaggg gatgacccag cgcctcggcc tcgcctccgc gctcgtccat
     4081 cgccccgacc tcctcctcct cgacgagccg accaacggcg tcgatccggt cgggcggcgc
     4141 gagatccgcg acctcctccg ggaggaggcg gcgcgcggcg cggcgatcct cctgaactcc
     4201 cacctcctct cggagatcga gatgacgtgc gaccgcgtgg ccgtcctccg gaaagggaag
     4261 gccgtcgccg ccgggcggat cgccgacctg accgcgcagg ggttcgccta tcgcttcgtc
     4321 ggcgccccga tggacgagga gctcctcggc ctcttccgcg acgcgggagc cgcggccgag
     4381 cgcgtgaacg gccacgtgaa gctgacggcg cgcgacgccg agcacctcaa cacgctcgtc
     4441 gacgcggcgc gcgcccgcgg cgtcctcgtc accgagctga ccccgcttcg atcgacgctc
     4501 gaggacgtct tcgtcgacct ggtctcggcg ggcgatccgg gcgaggcggg cgggacggtc
     4561 cacgggtcgc agggagaagg gaggccccgg tgaaggtcct cgccgcgaac gtcgaggagg
     4621 tcttccgcga ggccgcggct cgctggaccc tcgtcgccta cggagccctc tcgacgctct
     4681 tcatcgccct cttcgccgcc gccgtgaacc tcgacgtcgt cgacggcgcg ctcgccgggg
     4741 cgaagctctt cggcaagcag ctcgagatcg ggccacggtc ggtcgacctc gacaagctcg
     4801 tcctcgggtt cgagtcgggc ttctcggggt tcctctacgt cgtggcgacg ttcctcgcgg
     4861 tcttcgcgac ggcgcacctc gtgccgaggc tccaggagaa ggggacgatc gacctctacc
     4921 tctcgaggcc cgtcggccgc gtgccgctcc tcctctcgcg ctacgtcggc gggcttctcc
     4981 tcgccggcct gaacgtcgtc tatctcatcg gcgcgatggg gctcctcatc tggtggaaga
     5041 cgggcgtcgt ccaccctcga ttcttcctcg gcggcgcgat catcctcttc acgatcgcga
     5101 cgctgatggc gttcgcgttc ctcgtcggcg tcgtgacgtc gtccaccgcg gtctcgatca
     5161 tggcgacgtt cgccgtcttc ttcctcgccg cgatcctctc gggccacagg cagatcgaag
     5221 ccgcgctcgc gacggaatgg gcggcctccc tcgtgcgcgg cttctactgg gtcctcccga
     5281 agacggggca gctcgcccag gcgaccgtcg actacgtctc cggcggcgag ctgtcgagac
     5341 acaaggtcga gatcgactcc ctcgccgtct tcgggacgac cggcctcttc ggtgtcgtct
     5401 ccctcgccct ctccgccctc ctcttccgga gaaaggactt ctgacatgaa gcgcannnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnctcgt cgcgttcgag gggcggttcg
     5581 agccccggcg tctcgccgcc gccgccgccg cgcgtggcgc ggaggcccgg acggccgccg
     5641 gcgcgaccta cttcctcctg aaggacgacc ggcagaccgg gaagcggggc ggcggagcgg
     5701 tcgccttcgt cagcgaccgg ctcctcgtgg ccggctccga ggaaggcgtc gcggcggcgc
     5761 tcgccgcccg cgccgcggga ggcaccggct tcgaccgcgg cgcagggctc ggcgcgtggc
     5821 tcgagagggt gaacggcaac gcctccggct ggctcctcgt cgaccggacg aagctgcccg
     5881 aggcgaaggg gtcggtcacg ctctccggga acgacggcgc cgtgcggaac gtgatcggcg
     5941 cgatgaacct cgtctcagcc ttcacgctcg agatgacggt gaagggggat gcgatccgcc
     6001 tcgccggagc gggatacagc gacgacaccg agacccgcga gctcctcgag gacgcgctcc
     6061 gcggcatcct cgcgacgatg cggatcgcgg cccaggagaa gccggacgtc gtttcggcga
     6121 tccggaagtt caaggtcgcg tcggaccgga acgccgtgac gatctccggc acgctcgacg
     6181 gggcgacgat caaggccctc agcgcggcga aggccgagcg cgagaacgcg cgcgcggcgg
     6241 ggtcgaaggc cgcgacgaag taggccccgg ggacggaagc cccggctttc cgggagcgcg
     6301 cgcggggcct attcttcccg gccggaggtc ccgcgtgcgc acgattcgga cggtcgccct
     6361 cgtctccctc ctcgttccct tcacggccgg ggcgggcgaa actcccgcgg tgaagccacc
     6421 ggtcgccagg aagctcccga aggtcaccga gatccacggc gagaagctct cggacgacta
     6481 cgcctggctg agggagaagc aggctcccga ggtgaaggcg tacctcgacg ccgagaacgc
     6541 gtatgcggac gccgtcacgg cgccctccga ggggctccgg aagaagctct acgaggaggc
     6601 cgtcggccgc gtgaaggaga cggacctctc ggtcccgtac cggcaccgcg gcttctggtg
     6661 gtacgcgcgg acggagaagg ggctccagta cccgatctcc tgccggaaga aggagagcct
     6721 cgaggcgaag gaggaggtcg tcctcgacct gaacgaggtc gcgaagggcg aggcgttcgt
     6781 cgggcggggc ctcttcgagc cgagcgacga cgggaagctc ctcgcctaca cgatcgacac
     6841 cacaggcttc cgcctctaca ccctcttcgt aaaggacctg acgaccggga cgcttctccc
     6901 ggaccgggtc gagagggtcg actccgtcgc ctgggcggcc gacgggaaga cgctcttcta
     6961 cgtcgtcgag gactccgcga agcggcctca ccgcctctac cggcacgtcg tcgggacgac
     7021 ggggcccgac gcgctcgtct acgaggagaa ggacgagcgg ttcacgctcg aggtctcccg
     7081 ctcgcgcgac gaccgctgga tcctcgtgaa gtccgacagc cacacccaga gcgagtggcg
     7141 gctccttccg gcttcggagc cggaggcgga gctccgcgtc gttcagcccc gcgagccgga
     7201 ccacgagtac gacgtggagg ccgcgggcga cctcctctac atccggacga acggcggctg
     7261 ccgcgacttc cgcgtcgtga cggcgccggc ggcgagcccc gggaaggcct cgtggaagga
     7321 gctcgtcccg tgccgggacg gcgtcatggt ctccggcgtc gacgccttcc ggcggcacct
     7381 cgtcctctcc gagcggcagg acgcgcagcc gcgcctcgcg gtccgcgacc tcgcgacggg
     7441 agagacgcac cggatcgagc tccccgaggc gatcgcctcg gttcatccgg aggcgaaccc
     7501 cgagtacgac acgacgtcgt tccgcttcac gttccagtcc tacacgacgg ccccgatcgt
     7561 cttcggctac gacctcgcga cgcgggagcg gacggtcctg aagaagaccg aggtcccggg
     7621 cggctacgac ccgtcgcgct accggtcgga gcgggtcttc gccacggccc ccgacgggac
     7681 gaaggtcccg gtgagcgtcg tctcccggaa ggacctcccg cgcgacggga cggcgcccct
     7741 cttcctgacc ggctacggcg cctacggcta cccgtcgttc gcctcgttca gcccggcgct
     7801 ccccagcctc ctcgaccgcg gcgtcgtctt cgccgtcgcg cacgtccgcg gcggcggcga
     7861 cctcggcaag aagtggcacg acggcggccg gatgacggcg aagaagaaca ccttcaccga
     7921 cttcgtcgcg tgtgccgagg cgctcgtcgc ggcgaaggtc gcctcgaagg atcgcctcgc
     7981 gatccagggc ggcagcgcgg gagggctcct gatcggcgcc gccctgaacc tccgccccga
     8041 cctcttccgg gccgcgatcc tacaggtccc cttcgtcgac gtcatcaaca cgatgctcga
     8101 cgagacgctg cctctgaccg tcggcgagtt cgaggagtgg gggaacccga agaagaagga
     8161 cgagtacgcc tacatgcgct cgtacagccc ctacgacaac ctgaagaagg gggcgtatcc
     8221 gtcgatcctc gtgaagacct cgttcaacga ctcgcaggtc atgtactggg agccggcgaa
     8281 gtgggtcgcg aagctccgga cgctgaagac ggacgggaac ccgctcctcc tgaagacgaa
     8341 catggcgggc gggcacggcg gctcctcggg gcggtacgac cggctgaagg agaccgcgtt
     8401 cgaccaggcg ttcgtcctga cgcagctcgg cgtgccggac ctgccgacct cgatcccggt
     8461 cccggcgcgg ccggtccaca cctactcgat cgtcgcgcgc gacccggcga cgggccagct
     8521 cggcgtcgcc gtccagtcgc actggttctc cgtcggggcg ctcgtcccgt gggccgaggc
     8581 gggcgtcggc gccgtcgcga cgcagtcgtt cgtcgacgcg agctacggcc cgctcggcct
     8641 ctcgctcctg aaagcggggc ggagcgcgcc cgacgccctc gcgggcctcc tcgcggccga
     8701 cgcgggacgc gacgtacgtc aggtcgcgat gatcgacgcc gccgggcgcg tcgcggcgca
     8761 cacgggcgcg cgctgcgtcg aggcggcggg ccattacgtc gggaaggact tctccgtcca
     8821 ggcgaacatg atgcggagcg cctccgtctg gcccgcgatg gcgaaggcgt tcgaggagac
     8881 gagaggggac ctcgccgagc ggatgctcgc ggcgctcgag gcggccgagg ccgcgggagg
     8941 cgacatccgc gggaagcagt cggcggcgct cctcgtcgtc tccgggaacc cgacggggcg
     9001 cgcgtggcag gaccggctgt tcgacctccg cgtcgaggac agccaagcgc ctctccccga
     9061 gctccggcgg ctcgtgacgc tcgcgcgggc ctacgccctc atgaacgagg gcgacctcgc
     9121 cgtcgagcgg aaggacgacg ccgcggcgct gaaggcgtat tccgccgccc aggcgatcgt
     9181 ccccgggaac gccgagatga cctactggac ggcggtctcc ctcgtcggga tgggaaaggt
     9241 cgacgaggcg ctcccgctct tccggaaggt cttcgcgatc gaccggagct gggcggagat
     9301 gaccccgcgc ctcccgaagt cggggctcct tccggacgac ccggcgctcc tcgcgaggat
     9361 cctggccgaa gctccgggcg cgcggtaggg gttccgcccg gggctagaat cgccggcacg
     9421 ccgatgaaca gcgccttcgc tccgacgagc gggtcggcga catccggctc ggaggcgaag
     9481 accggaaccg agcgaggccc ggaggcctcg gcgcctccgc gcgacgattc cggccccgga
     9541 ttccgccagc tcgccgaggt ggcgccgatg gggatcctcg tcgtcggcgc ggacgagggg
     9601 ctccgctacg ccaacccgag cgccgccgcg atgctcggcc gctcggtcga cgacctcctc
     9661 gcatgtcgct acgggaccct cgccgacccg tcctgccgcg acctcctccg gcagcggatc
     9721 gccgatcgaa tcgccggcgc tcccgtcccc gggacctacg agacgctctt ccgccgggcg
     9781 gacgggtcga gcgtcgcggt ggagctgagc gggaacctgc tgggctggca cggcgagccc
     9841 tgcgacctcc tcttcctctc ggacgtcacg gtgcgccgga acgcgctcgc ggccctcgag
     9901 gaggagcggg aggccttccg ctcggtcttc gagaacgccg gcgaggcggt cttcctgacc
     9961 tctccggacg gctcgatctc cgcggcgaac ccggcggcct gccggatgct gggccggagc
    10021 gaggaggaga tccgtcggct cggtcgttcg ggggtcctcg acctgtccga cccgcgtgtc
    10081 ccggcggccc tcgaggagcg cgcccggacg ggccggttcc acggagagct ccgcgcgctg
    10141 cgggtgagcg gggaggcgtt cccggccgag atcacctccg atctcttcac cgacgcccac
    10201 ggaaagccgc gatcggcgac catcctccgc gacgtgacgg aacgaaggcg ggccgaggag
    10261 gcgttccggc ggagcgaggc gctcgtccgc tcgctgaacg cggagctgga gcagcgcgtc
    10321 gccgagagga cggaggcgct cgccgcggcg aacgaggagc tgcgtggctt ctcgagctcg
    10381 atcgcgcacg acctgaccgc ccctctccgc gggatcaacg ggcttgccgg acggctcctc
    10441 gaggaccacg ggtcgaacct gggcgaggag gcgcgggggc tgctccggag gatcgtcgag
    10501 gcgagcgcgc gcggcgggag gctcattgag gcgcttctgg accacgcgag gatctcggtg
    10561 gcgaggctgg agcgggaggc ggtcgacgtg ggggacctcg ccgcggcgat cctccggcgg
    10621 cgcgcggcgg aggagcccgg tcggaacgtc acctggatcg caccctccgg cctcgacgct
    10681 cgcgcggacc gccgcctcgt cggcgagatc ctggagcacc tcctcgcgaa cgcgtggaag
    10741 ttcacgtcgc cccgcgaggc ggcccgcatc gaggtggggg cggcgacgga ggggggcgag
    10801 acggtcttct tcgtccggga cgacggcgtt ggcttcgagc cagcgcatgc cagccgcctc
    10861 ttcgagctct tccaccgggc ccacgcgggg acggagctgt cgggcctcgg cgtggggctg
    10921 gcgctcgcgg agcgtgccgt ccgctgccac ggcgggtgga tccggatcga cggagcgccg
    10981 ggagccggcg cgacggtccg cttcacgctt tcgcctcgcc gctgagccgc gcacggcggc
    11041 cgcgatccgg ccgcggggtg ccgcttcgcg gcccggatgc gtgcggctca gtccggcgac
    11101 ttcggcgagg ggacgggctc gtccatcggc ccgtcgccgg gaacggtctc gagggccttc
    11161 ctcacccgcg cgagctcggc gccgatccgg tcgaggagcg gctcgagggt cgatgtctcg
    11221 ttctccttcg cgcgcgcttc cgcttcgcgg cagagcgcgg agaggtcgag cgcgccgaag
    11281 ctcgcgctcg tcgagcgcag cgtgtggacg gcccggtgga gctcgtccgc gcgttcggcg
    11341 tcggccgcgg accgggcggc ggcgaggagc ccgtcggatt cccgggcgaa ggagcggacg
    11401 atgcccggga ggaggtcgac cgcccgggcg ccgagcgagg cgtggagacg cgcccaggcc
    11461 ttgccgtcga ggccgggcgg cgtcgcgagc ggcggcgtcg gggagggcgt cggggtgacg
    11521 ggcacgtcgt cgccgtcccc ttccgtcagg ggggagagcg cggtcgcggc gcgctcgagc
    11581 gccgcgacga ggtccgagat cccgatgggc ttcacgaggt agtcgttcat gccggccgcg
    11641 aggcacgcct cgcgatcgcc cggcagggcg tgggcggtca tcgccgcgat tcgggggccg
    11701 tcggctccca gcgcggcgcg gatctggcgg gtcgcttcga tgccgtccag ctcgggcatc
    11761 tggacgtcca tcaggacgag gtcgaagacc cgggaagaga ccgcctcgac cgcctcgcgg
    11821 ccgttcgtgg cgacgtcggc cgagtagccg agccggccga gcatgaggag cgcgagctcc
    11881 tggttcgtcg ggttgtcctc gacgaggagg atccggagag ggagccggcg gcccatcgcg
    11941 ccgtcgaacc gcgggcggat cgcggactcg cgcctctggg ccggacggct cccgccgagc
    12001 agggcggcga cggcctcgat gaggacggcg ggcttgacgg gtctcgtcac gcgtgcgacc
    12061 gggccgtcca cgccttcctc ggcctcggcg cccatcggga cgaccaggac gaaggggagg
    12121 gacggtgcga agcgggatcg gaggcccgtg aaggcggcga ggccgtcgcc ctcgggcaga
    12181 tcgcggtcga cgacggcgag gtcgaagcgc tcgccggacc ggaagcgctc cgccgcagcg
    12241 gcgatcgagc cgctggccgc cacctggaga cctcgtcccg acagggtctt cgcgaggagc
    12301 gcccggctcg tctcgcgctc ctcgacgacg aggacccgcc gcccggcgaa gctcgcgcgc
    12361 cgcgacggga cgatggcgcc agaaggaggc atcgccgcct cgaggcgggc gacgaatcgg
    12421 aacgtcgagc cgcgccccgg ctcgctctcg aacgcgatcg agccccccat cagctcggcc
    12481 aggcgccgcg agatcgcgag gccgaggccg gtgccgccga agcgccgggt cgtggagctg
    12541 tccccctggc ggaacgattc gaagaggttc gcggcgcgct ccggacggat gccgatgccg
    12601 gtgtcgcgga ccgcgacctc gagctcgacg agcgtccccg gaggagggct gcccgaggga
    12661 cgggagacga cggtgacggc gacctccccg gcgtcggtga acttcacggc gttcccgacg
    12721 aggttcatga ccacctgctt catgcgcgtc acgtcgccca ggagcgccac cgggacgccg
    12781 tcgtcgacgt cgagcgtcag ctcgaggcgc ttctcctcg
//
LOCUS       DAOTEZ010000074        12655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3494_length_12655_cov_7.095397, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000074 DAOTEZ010000000
VERSION     DAOTEZ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12655
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3494_length_12655_cov_7.095397"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="P5164_05800"
     CDS             complement(<1..190)
                     /locus_tag="P5164_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43442.1"
                     /translation="MGLFGKSLDEKAAEAVKSLRGSIKGLKSLDAVVEGKTVTLTGEA
                     DSIEAKGKAMAEFNKLVET"
     gene            complement(257..1354)
                     /locus_tag="P5164_05805"
     CDS             complement(257..1354)
                     /locus_tag="P5164_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRY43443.1"
                     /translation="MSSVPIPPAPPVTNARVASALLALADAEPPGARPAVEYRSAAAT
                     LRQLPDELHGRRIHLGGLPHVTPRAADAIAAFLSCGSDESVGDRLSALLDREDPASER
                     KREYLSRADVDRILAAPEGISPADLRADVHLHTDRSDGTIPLAELHRALAGRGDRYAL
                     LTDHARDLAVAGGLHAGDFAAQARELERLNARTGEEFGMLLGAEANIAEDGTIDVLPA
                     NVPVLAAVVASVHTELRGPKDQTARLVRAVERPGVAILGHPKGRLYNMRTGVRASWDR
                     VFAAAAERGVAIEVNGCPERQDLPPSLARTALAAGCLFALTSDAHAHRHLAFQRFSLA
                     IARLAGIPAARVVNAWPRERFEEWLEERRQG"
     gene            1409..4222
                     /gene="polA"
                     /locus_tag="P5164_05810"
     CDS             1409..4222
                     /gene="polA"
                     /locus_tag="P5164_05810"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRY43444.1"
                     /translation="MPEKKTLALLDASGYLYRAFHAIRPLTAPDGRPTNATFGFATML
                     RKLLSQRKPDAVAVCFDRPEKTFRHEMDPEYKATRSAMPDDLVPQVADVKGLCRAMGL
                     VVVEEPGFEADDLIGTLAEKGARAGFAVEVVSADKDLFQLVREGEVAVWHPVHEKLLD
                     SEGVKELFGAPPERVADVLGLMGDSSDNIPGVRGIGEKGAKELVATYGPLEEIYARVG
                     ELKGKRREALEAGRDDAFRSRELATVRRDAPTPGGESAESLLGTFAVPPASPDGIAEL
                     AAFFGELGFSRLKKELLEAAGTTAPRPAAKPAGSGSLFGEEDAEAESGRTAEASAPTI
                     TAGGSGDLPSWESSPASLDAFLVRAEASGRVGLHVECAPGRPFPPEPIALAVALPGGA
                     SLAAPLAGDGSEAGRAFLARLLAHEAVEVVAHESKRLHLAADALSLPLPRRVFDAMLA
                     AYVDSPGLHAHDLAGDARALLGRDPSAVPSPKEVAGSDAFETAAELATEAGRAYLGPR
                     ARIPLELGDVLLARLTARPASLRVYEELELPLVPVLARMELAGILVDPAALSVLSVDL
                     GARLAALEAEIHAAAGEPFNVGSPLQLGRILFEKLGYPALKKTAKTKSYATGSEILEE
                     LTVRGFGPLPGLVLEWRELSKLKGTYVDALPKHVAKDGRIHTRFDQAVAATGRLSSND
                     PNLQNIPVRTEAGRAIRKAFVAPKGRLLVSADYSQVELRLLAHLSGDEALIETFRRGD
                     DIHRATAARIFGIHPDLVSPDQRRGAKTINFGILYGMGPFALAGQLGVTQGEAKAFIA
                     AYFQQFPSIRACLDRILAQARETGATETIFGRLRPIPGIADRNHAVRANAERMAMNAP
                     FQGAAADLIKKAMVDLDARLSAELPSARMLLQVHDELVLECDEGDAARAADLARETME
                     GAASLAVPLTVETARGPDWAAAK"
     gene            4243..5133
                     /locus_tag="P5164_05815"
     CDS             4243..5133
                     /locus_tag="P5164_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRY43445.1"
                     /translation="MAFVGRRTLRLFLPCLPALLLAPVLSAQALLETIPDPVRLAPAE
                     VPFVNPLWQALDAPAAPKGPPPRLRDLETTGPLIVGVKLDASGAVSEAVVPEPPLRAL
                     AAAAKAQAPRWTFQPATKDGVAVRCWGTYGVDVEVELEDATWHAFALVPVGKDDPLPE
                     LPKELPGESWMSRYPAAVTPPEPGVFSVEEVDFLPMPAKVPLKLEGARLKSRLLALVE
                     VSGLGSVKRVVPVGTYEPMILRWVRESAKEWTLAAARKGGSPVSSWLALDTTIEYSLG
                     SVKERGKRSIKKNVRGEPAP"
     gene            5130..5831
                     /locus_tag="P5164_05820"
     CDS             5130..5831
                     /locus_tag="P5164_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRY43446.1"
                     /translation="MKLTTADAADLLDVDESTIYRWIEERDLPAERIAERTLLNRAAL
                     LEWATEHRVAVSPRVFLPAEAGGERLPSFAAALAAGGVHHALPGGTRQEVLREAVSRL
                     PLPEGIEAEELLAFLLARETAGSSAIGDGIAIPHVRAPIVLDVDEALVSLSFLAEPVD
                     FGASDGRPVTVLVTMVTPTVHGHLHLLAALACLLRDEGFRAAVKAAAPAEELLALARE
                     IEARGVGRRAETGAA"
     gene            5831..7879
                     /locus_tag="P5164_05825"
     CDS             5831..7879
                     /locus_tag="P5164_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRY43447.1"
                     /translation="MEAASPGGVLLLGAIALVALSGLPGLIARKGGSGGERLSALLVG
                     AGALLGLVASARVLFLGRSFRVELPSPLPGGPLLLAADPLSALFLVPVFLVPALGAVY
                     GLGYWPEAEHRRDARKLRFFYGLLAASMAVVLLARSGVLFLVAWEVMALAAFFAATTE
                     DRQPEVREAGWIYLVATHAGTLGLFGMTALLARTTGGFAWEAPEGGLAVTASSSVVFL
                     LAVVGFGGKAGLFPFHFWLPGAHGGAPSHVSAVMSGVMLKVGLYGLLRVVSLYGAPPA
                     WWGGLLLLLGAGSALFGAALAAGQGDVKRLLAYSSVENVGIVTTGIGLALLGRSAGRP
                     EWVALGLGGALLHVLYHSLFKPLLFLGAGAVIHGAGTREMDRLGGLLRRMPRTGAAFA
                     AGCAAALAFPPLAGFFSELVVYLGLFRAFAGPDSSLLAAFAAPALAVAGAVALAAFTK
                     LFGVAFLGSPRSDEASHAHEPGRAMLVPILALALLVAVAGLLSPLLAPGVDAAIRAWV
                     APAVPPGSVEALLHPVPPLASLVPLGTIALLGAALLGLLALFAGLLALRLRRASVARG
                     ATWDCGYARPTARMQYTGSSFGDWISGRLTPAAAAPVLEVRRPEGSFPRSAAVSAPRA
                     RDPLLRRLLEPFASRWARRFHDLHALQEGRLTIYLVYVLGTLVALLAWSALRGWVLPR
                     "
     gene            7876..9858
                     /locus_tag="P5164_05830"
     CDS             7876..9858
                     /locus_tag="P5164_05830"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRY43448.1"
                     /translation="MSLLLVLGGALLCAAGGVPGLFLRRVETGRRLAVAGSVSGSLLG
                     LAGAALAAATGTVATIHRAWPIPGAAFHVSLDALSALFLAPLFLVSGVAAVSGARSFG
                     DEEHPDDAPRTRLFFGLATAAIALVLLARNLVLFLASWEVMALSAFFLVTSDRSSREA
                     RDAGRLYIFATHVSTLVLFAFFALFRSLSGSTELVTLPAGVAGSDAGTALFVLALLGF
                     GLKAGLLPLHVWLPPAHAAAPAHVSALMSGVLIKTGIYGIVRFTSLVPDPPASWGDGL
                     LLLGAASAVLGVAFALGQHDLKRLLAWHSVENIGIIVLGLGVGLGARAAGRPEWALLG
                     FAGGLFHVVNHGLFKPLLFLGAGEAAHVAGTRDMDRMGGLGKLLPKTALLFAAGAVAI
                     SGLPPLNGFASEWLVYLGLFDRARATGTDALRAAFGVPALALTGALALACFVKAHGAV
                     FLGNARAEARQLHGQPASHGEPAAHVGPMALLAVACALLGLLPATVAPALERAAAIAA
                     PGLAASRPLAELASLRTVGVAALTVVLLLGAVALFLRRRLAAETMAGPTWDCGYARPT
                     ARMQYTASSFARGPVGWFGWALRPRLKGAPVAKLFPTAAQFESHVLDTVLDRAVVPVF
                     RAGAWLARQGRVLQHGRMQLYLLYVVATLVALLFQV"
     gene            9872..10774
                     /locus_tag="P5164_05835"
     CDS             9872..10774
                     /locus_tag="P5164_05835"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HRY43449.1"
                     /translation="MIDTLVHLALVLALPPLLTGVIAKTKAAFAGRNGPPLLQGYWDL
                     AKLLRKGSVLPAGSTWVFLAGPVAGVAAPLLASLLLPFGGHEAPLSFAGDMILFAYLF
                     ALARFFTASAALDTGSAFEGMGAAREVAFSALAEPALFFGFVALARISGSLSLSGMLG
                     SGPGWVTAGAPLVLVAASWAVVLLAENCRIPFDDPNTHLELTMIHEVMVLDHGGPALG
                     LVLYGASVKLFVLSALFLRVVFPFNATDPNLDWLAFVAGILLVAIGIGVVESVMARLK
                     LVKVPQLLVGACLLSAFGMLLLAR"
     gene            10779..11420
                     /locus_tag="P5164_05840"
     CDS             10779..11420
                     /locus_tag="P5164_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HRY43450.1"
                     /translation="MTHPVDILLVLVILLNFLVLGTPSLRMTIRGAALQGVLLALLPL
                     LVHRGFGWRVGAIAVATMALKGLLIPGLLEKAMRDVHIRREVEPYVGFVPSLFLCAVG
                     TALAILFSGNLPLAPEHAGTLLVPASLATLLSGFLVLTTRKKAISQVVGYLILENGIF
                     IFGQLLIEAMPFLVEAGVLLDLFVGIFIMGIVINHIRQEFSSLDTELLSELKD"
     gene            11434..>12655
                     /locus_tag="P5164_05845"
     CDS             11434..>12655
                     /locus_tag="P5164_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRY43451.1"
                     /translation="MEVGLVLLPLLGAVVAALVRSRERRAWVVPAAGVLHLLLLGGMV
                     LGNVRSGRGFWLRLDPLATVILGTISVLFVVAAFYLPGYLALRRDRDDRVFCAGLLVF
                     LAMTSLLALAQHLGLLWVAMEATTLATAPLLYFNRTARSLEATWKYLMIGSVGIAIAL
                     LGSLFLAYSAHVGGAEPSLLFDDLLAGAGSFSRPWLRAAFVLLLVGYGTKMGLAPLHT
                     WKPDAYGEASGIVGALLAGGMTNAAFLALLRVYRVVNAAGEGAFARELLVFMGLVSIA
                     LAAVFVVRQRDLKRMLAYSSVEHVGILVLGIGLGGIGVFGSLLHAINNGLTKGVLFLS
                     VGNIHRAFGSKHLDEVSGAISRLPVSGSLFLVGFFAITGSPPFGPFVSEFTIVNAAIG
                     GGHAVAGTLLLVLLG"
BASE COUNT         1336 a         4945 c         4384 g         1990 t
ORIGIN      
        1 cggtctcgac cagcttgttg aactcggcca tggccttgcc cttggcctcg atgctgtccg
       61 cctcgccggt cagcgtgacg gtcttcccct cgacgaccgc gtcgaggctc ttcagcccct
      121 tgatcgagcc gcgcaggctc ttcaccgcct cggcggcttt ctcgtcgagc gacttgccga
      181 aaagacccat ctttgcctcc tctgccggat tccggggaga gaagacgata tcaggagcgt
      241 cgcgcgcccg gacggctcag ccctggcgtc tttcctcgag ccattcctcg aaacgctcgc
      301 gcggccaggc gttgacgacg cgcgccgccg ggatcccggc gaggcgcgcg atcgccaggg
      361 agaagcgctg gaacgcgagg tggcggtggg cgtgggcgtc gctcgtcagc gcgaagaggc
      421 agccggcggc gagggccgtc cgggcgagcg acggcggaag gtcctgccgt tcggggcagc
      481 cgttgacctc gatcgcgacg ccccgctcgg ccgcggccgc gaagacgcgg tcccagcttg
      541 ctcgcacgcc ggtccgcatg ttgtagaggc gccccttcgg gtggcccagg atcgccacgc
      601 cggggcgctc gacggcgcgg acgagccggg ccgtctggtc cttcgggccc cgcagctcgg
      661 tgtggaccga ggcgacgacg gccgcgagaa cggggacgtt tgccggcagg acgtcgatcg
      721 tcccgtcctc ggcgatgttc gcctcggcgc cgaggagcat cccgaactcc tcccccgtcc
      781 gtgcgttcag ccgctccagc tcgcgcgcct gcgcggcgaa gtcgccggcg tgaaggccgc
      841 ccgccacggc gagatcgcgc gcgtggtcgg tcaggagcgc gtagcggtcg ccgcggccgg
      901 cgagggcgcg gtggagctcg gcgagcggga tcgtcccgtc ggagcggtcg gtgtggaggt
      961 ggacgtcggc ccggaggtcg gcgggcgaga tcccctccgg cgccgcgagg atccggtcca
     1021 cgtcggcgcg ggagaggtac tcgcgcttgc gctcgctcgc cgggtcctcg cggtcgagga
     1081 gggccgagag ccggtcgccg accgactcgt ccgacccgca ggagaggaac gcggcgatcg
     1141 cgtcggcggc gcggggcgtg acgtgcggca ggccgccgag gtggatccgc ctcccgtgca
     1201 gctcgtcggg gagctggcgg agcgtcgccg ccgccgagcg gtactcgacg gcggggcgcg
     1261 cgccgggcgg ctcggcgtcg gccagggcga ggagggcgga ggcgacccgg gcgttcgtga
     1321 cgggcggggc ggggggaatc gggacggacg acaccggcgg gcctcgcccc atcttacggt
     1381 cccgccctcc cgatagactc cggtcgacat gccggaaaag aagacgctgg cgctcctcga
     1441 cgcctcgggc tacctctacc gcgccttcca cgcgatccgc ccgctcaccg cccccgacgg
     1501 gcggccgacg aacgcgacgt tcggcttcgc gacgatgctc cggaagctcc tctcccagcg
     1561 gaagcccgac gccgtcgccg tctgcttcga ccgtcccgag aagacgttcc gccacgagat
     1621 ggacccggag tacaaggcga cccgctcggc gatgccggac gacctcgtcc cgcaggtggc
     1681 cgacgtgaag gggctctgcc gcgccatggg cctcgtcgtc gtcgaggagc cgggcttcga
     1741 ggccgacgac ctgatcggga cgctcgccga gaagggcgcg cgggccggct tcgccgtcga
     1801 ggtcgtctcg gccgacaagg acctcttcca gctcgtccgc gagggcgagg tcgccgtctg
     1861 gcatcccgtc cacgagaagc tcctggactc ggagggagtg aaggagctct tcggagcgcc
     1921 gccggagcgc gtcgccgacg tcctcggcct catgggcgac tcgtccgaca acatcccggg
     1981 cgtccgcggg atcggcgaga agggggcgaa ggagctcgtc gcgacgtacg gccccctcga
     2041 ggagatctac gcgcgcgtcg gcgagctgaa ggggaaacgg cgcgaggcgc tcgaggcggg
     2101 gagggacgac gcgttccgct cgcgagagct cgcgaccgtc cggcgcgacg cgccgacccc
     2161 gggcggcgag agcgcggaga gcctcctcgg gacgttcgcc gtccctccgg cgtcgccgga
     2221 cgggatcgcc gagctcgccg ctttcttcgg cgagctcggc ttctcgcgcc tgaagaagga
     2281 gctgctcgag gcggcgggga ccacggcgcc gcggccggcg gcgaagcccg cgggaagcgg
     2341 gtcgctgttc ggcgaggaag acgcggaggc ggagtcgggc cgaacggcgg aggcgtccgc
     2401 cccgacgatc accgcgggcg ggtccggaga cctcccctcc tgggagagct cgcccgcctc
     2461 gctcgacgcc ttcctcgtgc gggcggaggc ctctggccgc gtcggcctcc acgtcgagtg
     2521 cgcgccggga cgcccgttcc ctcccgagcc gatcgccctc gccgtcgcgc ttcccggcgg
     2581 cgcctcgctc gccgcgccgc tcgcgggcga cggttccgag gccggccgcg ccttcctcgc
     2641 gcggctcctc gcgcacgagg cggtcgaggt cgtcgcccac gagtcgaaac gcctccatct
     2701 cgccgcggac gcgctctccc tcccgcttcc acgtcgcgtc ttcgacgcga tgcttgcggc
     2761 ctacgtcgac tccccggggc tccacgccca cgacctcgcg ggcgacgcgc gcgccctcct
     2821 ggggcgcgac ccgtccgcgg tgccgtcgcc gaaggaggtc gccgggagcg acgccttcga
     2881 gacggcggcc gagctcgcga ccgaggcggg acgcgcctac ctcggcccgc gcgcccgcat
     2941 tccgctcgag ctcggcgacg tcctcctcgc gcgcctgacg gcccgtcccg cctcgctccg
     3001 cgtctacgag gagctcgagc tcccgctcgt gccggtcctg gcgcggatgg agctggcggg
     3061 aatcctcgtc gacccggcgg ccctctccgt cctctccgtc gacctcggcg cccgactcgc
     3121 ggcgctcgag gcggagatcc acgcggccgc gggggagccg ttcaacgtcg gctccccgct
     3181 gcagctcggc cggatcctct tcgagaagct cggctacccg gcactgaaga agacggccaa
     3241 gacgaagagc tacgcgaccg gctccgagat cctcgaggag ctcacggtgc gcggcttcgg
     3301 cccgcttccg ggactcgtcc tggagtggcg cgagctctcg aagctgaagg ggacctacgt
     3361 cgacgcgctg ccgaagcacg tcgcgaagga cgggcggatc cacacccggt tcgaccaggc
     3421 ggtcgcggcg acggggcgcc tctcctcgaa cgacccgaac ctccagaaca tcccggtacg
     3481 gaccgaggcg gggcgcgcca tccggaaggc cttcgtcgcg ccgaaggggc gcctcctcgt
     3541 ctcggccgac tactcgcagg tcgagctgcg cctcctcgcc cacctctcgg gcgacgaggc
     3601 gctcatcgag accttccggc gcggggacga catccaccgc gccaccgccg caaggatctt
     3661 cggcatccac cccgacctcg tctcgcccga ccagcggcgc ggggcgaaga cgatcaactt
     3721 cggcatcctc tacggcatgg gcccgttcgc cctcgccggg cagctcggcg tgacgcaggg
     3781 agaggcgaag gcgttcatcg ccgcctactt ccagcagttc ccctcgatcc gcgcctgcct
     3841 cgaccggatc ctcgcgcagg cccgcgagac cggggcgacg gagacgatct tcggacgcct
     3901 ccggccgata cccgggatcg ccgaccgcaa ccacgccgtc cgcgcgaacg cggagcggat
     3961 ggcgatgaac gccccgttcc agggcgcggc ggccgacctg atcaagaagg cgatggtcga
     4021 cctcgacgcg cgcctctctg cggagctccc gtcggcgcgg atgctcctgc aggtgcacga
     4081 cgagctcgtc ctcgagtgcg acgaagggga cgccgcgcgc gccgccgacc tcgcgcggga
     4141 gacgatggaa ggcgccgcgt cgctcgccgt cccgctgacg gtggagaccg ctcgcggccc
     4201 cgactgggcg gccgcgaagt agcgggagct acaatccgaa agatggcctt cgtcggccgc
     4261 cggacgctcc ggctgttcct tccctgtctc ccggccctcc tcctcgcgcc ggtcctttcc
     4321 gcgcaggcgc tgctcgagac gatccccgat ccggttcgcc tcgcgccggc cgaggtgccg
     4381 ttcgtcaacc cgctctggca ggccctcgac gccccggcgg cgccgaaggg gccgccgccg
     4441 cggctccggg acctggagac gaccggcccc ctgatcgtcg gcgtgaagct ggatgcctcg
     4501 ggggccgtct cggaggcggt cgtccccgag cccccgctcc gcgcgctcgc cgccgccgcg
     4561 aaggcccagg cgccgcgctg gacgttccag ccggcgacga aggacggggt ggccgtccgc
     4621 tgctggggga cgtacggcgt cgacgtcgag gtggagctcg aggacgccac ctggcacgcg
     4681 ttcgcgctcg ttccggtcgg gaaggacgac ccgctcccgg agctgccgaa ggagctcccg
     4741 ggcgagtcgt ggatgagccg gtacccggcc gcggtgacgc cccccgagcc gggcgtcttc
     4801 tcggtggagg aggtcgactt ccttccgatg ccggcgaagg tcccgctcaa gctcgagggg
     4861 gcgcgcctga agtcgcgcct cctcgcgctc gtcgaggtct cggggctcgg gtcggtcaag
     4921 cgcgtcgtcc cggtcgggac gtacgagccg atgatccttc gctgggtccg cgagagcgcg
     4981 aaggagtgga cgctggcggc cgcgcggaag gggggctctc cggtctcctc ctggctcgcg
     5041 ctcgacacga cgatcgagta ttccctcggc tcggtgaagg agcgcgggaa gcgttccatc
     5101 aagaagaacg tccgcggcga gccggcgccg tgaagctgac gaccgcggac gccgccgacc
     5161 tcctcgacgt cgacgagtcg acgatctacc gctggatcga ggagcgggac cttcccgccg
     5221 agcggatcgc ggagcggacg ctcctgaacc gcgcggccct cctcgagtgg gcgacggagc
     5281 atcgcgtcgc cgtctcgccc cgcgtcttcc tccccgccga ggcggggggc gagcggctcc
     5341 cctccttcgc cgcggcgctc gcggcgggcg gagtccacca cgccctcccc ggcgggaccc
     5401 ggcaggaggt ccttcgcgag gcggtctcgc gcctcccgct ccccgagggg atcgaggccg
     5461 aggagctcct cgccttcctc ctcgcgcgcg agacggccgg ctcctcggcg atcggcgacg
     5521 gcatcgcgat cccgcacgtg agggccccga tcgtcctcga cgtcgacgag gcgctcgtga
     5581 gcctttcttt cctggccgag ccggtcgact tcggcgcgtc cgacggccgt ccggtgaccg
     5641 tcctcgtcac gatggtgacg ccgaccgtcc acgggcacct ccacctcctc gccgccctcg
     5701 cctgcctcct ccgcgacgag gggttccgcg cggccgtgaa ggccgccgcg ccggccgagg
     5761 agctcctcgc gctcgcgcgg gagatcgagg cgcgcggcgt cgggcgccgg gcggagaccg
     5821 gggccgcctg atggaggctg cgagtcccgg gggcgtgctc ctcctcggcg cgatcgccct
     5881 cgtcgcgctg agcgggctcc ccgggctcat cgcccggaag gggggatcgg gaggggagcg
     5941 gctctccgcg ctcctcgtcg gggccggggc gctcctcggc ctcgtcgcgt cggcgcgcgt
     6001 cctcttcctc ggccgcagct tccgcgtcga gctcccgagc ccgctcccgg gcgggccgct
     6061 cctcctcgcg gccgaccccc tctcggccct cttcctcgtc ccggtctttc tcgtcccggc
     6121 gctcggggcc gtctacggcc tcggctactg gcccgaggcc gagcaccggc gcgacgcccg
     6181 caagctccgc ttcttctacg gcctcctcgc cgcgtcgatg gcggtcgtcc tcctcgcccg
     6241 gagcggcgtt ctcttcctcg tcgcctggga ggtgatggcc ctcgccgcct tcttcgccgc
     6301 gacgaccgag gaccggcagc cggaggtgcg ggaggcgggg tggatctacc tcgtcgcgac
     6361 gcacgcgggc acgctcggcc tcttcggcat gacggccctc ctcgcccgga cgacgggcgg
     6421 cttcgcctgg gaggcgccgg agggcggcct cgccgtgacg gcctcctcga gcgtcgtctt
     6481 cctcctcgcc gtcgtcggct tcggcggaaa ggcgggcctc ttcccgttcc acttctggct
     6541 cccgggcgcg catggcggcg cgccgagcca cgtctcggcc gtgatgtcgg gcgtgatgct
     6601 gaaggtgggc ctctatggcc tccttcgcgt cgtctcgctg tacggcgccc ctcccgcctg
     6661 gtggggcggc ctcctcctcc tcctcggcgc cggctcggcc ctcttcggcg ccgcgctcgc
     6721 cgccgggcag ggagacgtca agcggctcct cgcctactcc tccgtcgaga acgtcggcat
     6781 cgtcacgacc gggatcggcc tcgccctcct cggccgctcg gcggggcggc cggagtgggt
     6841 cgcgctcggc ctcggagggg cgctcctcca cgtcctctac cactccctct tcaagccgct
     6901 cctcttcctc ggcgcggggg cggtgatcca cggggcgggg acgcgcgaga tggaccggct
     6961 cggcgggctc ctccggagga tgccgcggac cggggccgcg ttcgccgccg gctgcgcggc
     7021 ggcgctcgcc ttcccgccgc tcgccggatt cttctcggag ctcgtcgtct acctcggcct
     7081 cttccgtgcc ttcgcggggc ccgactcctc cctcctcgcc gccttcgcgg ccccggcgct
     7141 tgccgtcgcg ggagccgtcg cgcttgcggc gttcacgaag ctcttcggcg tcgccttcct
     7201 cggaagcccg cgctcggatg aggcgtcgca cgcgcacgag ccggggcggg cgatgctcgt
     7261 cccgatcctc gccctcgccc tgctcgttgc cgtcgccggc ctcctctcgc cgctcctcgc
     7321 gccgggcgtc gacgccgcga ttcgcgcgtg ggtcgcgccg gccgtgccgc ccggctccgt
     7381 cgaggcgctc ctccacccgg tgccgccgct cgcctcgctc gtcccgctcg ggacgatcgc
     7441 gctcctcggc gcggcgctcc tcggcctcct cgccctcttc gcgggtctcc tcgcgcttcg
     7501 cctccggcgc gcctccgtcg cgcgcggcgc gacgtgggac tgcggctacg cccggccgac
     7561 ggcccggatg cagtacacgg ggagctcctt cggcgactgg atctcgggcc ggctgacgcc
     7621 ggccgccgcc gcgccggtcc tcgaggtccg ccgccccgag gggagcttcc cgcggtccgc
     7681 ggccgtttcc gcgccgagag cgcgcgaccc gctcctccgg agactcctgg agccgttcgc
     7741 gtcgcgctgg gcgcgccgct tccacgacct tcacgccctc caggaggggc gcctgacgat
     7801 ctacctcgtc tacgtcctcg gcacgctcgt cgcgctcctc gcctggagcg cgctccgcgg
     7861 gtgggtcctg ccgaggtgag cctcctcctc gtcctcggcg gcgccctcct ctgcgcggcc
     7921 ggcggcgtgc cgggactctt cctccggcgc gtggagacgg ggcgccggct cgcggttgcc
     7981 ggctcggtct cggggtcgct cctcggcctc gcgggggccg cgctcgccgc cgccacgggg
     8041 acggtcgcga cgatccaccg ggcgtggccg atccccggcg ccgccttcca cgtctcgctc
     8101 gacgctcttt cggcgctctt cctcgctccg ctcttcctcg tctcgggcgt cgcggccgtg
     8161 agcggcgcgc gctcgttcgg cgacgaggag cacccggacg acgcgccgcg gacccggctc
     8221 ttcttcggcc tcgcgacggc ggcgatcgcg ctcgtcctcc tcgcgaggaa cctcgtcctc
     8281 ttcctcgcct cctgggaggt gatggcgctc tcggccttct tcctcgtcac gtcggaccgc
     8341 tcctcccgcg aggcgcgcga cgccggtcgc ctctacatct tcgcgacgca cgtctcgacg
     8401 ctcgtcctct tcgcgttctt cgccctcttc cgctctctct ccggctcgac ggagctcgtg
     8461 acgctcccgg ccggagtcgc ggggagcgac gcgggaacgg cgctcttcgt cctcgccctc
     8521 ctcggcttcg gcctgaaggc gggcctcctg ccgctccacg tctggctccc gccggcgcac
     8581 gcggcggccc cggcgcacgt ctcggccctc atgtcgggcg tcctcatcaa gaccgggatc
     8641 tacgggatcg tgaggttcac ctcgctcgtc cccgacccgc ccgcatcctg gggcgacggg
     8701 ctcctcctcc tcggcgccgc ctcggcggtc ctcggcgtcg ccttcgcgct cggccagcac
     8761 gacctgaagc gcctcctcgc ctggcacagc gtcgagaaca tcgggatcat cgtcctgggc
     8821 ctcggcgtcg gcctcggggc ccgcgcggcg gggcggcccg agtgggcgct cctcggcttc
     8881 gcgggggggc tcttccacgt cgtgaaccac ggcctcttca agccgctcct cttcctcggc
     8941 gcgggcgagg cggcgcacgt cgccgggacg cgcgacatgg accggatggg gggactcggg
     9001 aagctcctcc cgaagaccgc gctcctcttc gccgccgggg ccgtcgcgat ttccggccta
     9061 ccgccgctga acggcttcgc gagcgaatgg ctcgtctacc tcggcctctt cgaccgggcc
     9121 cgggcgacgg ggaccgacgc gctccgcgcg gcgttcggcg tcccggctct cgcgctgacg
     9181 ggagccctcg cgctcgcctg cttcgtgaag gcgcacggcg ccgtcttcct cgggaacgca
     9241 cgcgccgagg cgcggcagct ccacggccag ccggcgtcgc acggagagcc ggcggcgcac
     9301 gtcggcccga tggcgctcct cgccgtggcc tgtgccctcc tcggcctcct cccggcgacg
     9361 gtcgcacccg cgctcgagcg cgccgccgcg atcgcggctc cgggcctcgc cgcctcgagg
     9421 ccgctcgccg agctcgcctc gctccggacg gtcggcgtcg cggccctcac ggtcgtcctc
     9481 ctcctcggcg cggtcgcgct cttcctccgc cgtcgcctcg cggcagagac gatggccggc
     9541 ccgacctggg actgcggcta cgcgcgaccg acggcgcgga tgcagtacac ggcctcctcc
     9601 ttcgcgcgcg ggccggtcgg ctggttcggc tgggcgctcc gcccgcgcct gaagggggcg
     9661 cccgtcgcga agctcttccc cacggccgcg cagttcgaaa gccacgtcct cgacaccgtc
     9721 ctcgaccgcg ccgtcgtccc ggtcttccgc gccggcgcct ggctcgcccg gcaggggcgc
     9781 gtcctccagc acgggaggat gcagctctac ctcctctacg tcgtcgcgac gctcgtcgcg
     9841 ctcctcttcc aggtctgacc cgggggatcc gatgatcgac acgctcgtcc acctcgccct
     9901 cgtcctcgcg ctcccgcccc tcctgacggg cgtcatcgcg aagacgaagg ccgccttcgc
     9961 cggccggaac ggcccgccgc tcctgcaggg ctactgggac ctcgcgaagc tcctccgaaa
    10021 ggggagcgtc ctccccgcgg ggtcgacctg ggtcttcctg gccgggccgg tcgcgggcgt
    10081 cgcggctccg ctcctcgcct cgctcctcct gccgttcggg ggacacgagg cgccgctctc
    10141 cttcgcgggc gacatgatcc tcttcgccta cctcttcgcc ctcgcgcgct tcttcaccgc
    10201 gtcggcggcg ctcgacaccg gctcggcgtt cgaggggatg ggggccgcgc gggaggtcgc
    10261 gttctcggcg ctcgccgagc cggcgctctt cttcggcttc gtcgcgctcg cgcggatctc
    10321 cggctccctc tcgctctccg ggatgctcgg ctcggggccc ggctgggtga cggccggcgc
    10381 gccgctcgtc ctcgtcgcgg cgagctgggc cgtcgtcctc ctcgccgaga actgccggat
    10441 cccgttcgac gatccgaaca cccacctcga gctgacgatg atccacgagg tgatggtcct
    10501 cgaccacggc gggccggcgt tgggcctcgt cctctacggc gcgtcggtga agctcttcgt
    10561 cctctcggcg ctcttcctcc gggtcgtctt ccccttcaac gcgaccgacc cgaacctcga
    10621 ctggctcgcc ttcgtcgccg ggatcctcct cgtggcgatc ggcatcggcg tcgtcgagtc
    10681 ggtcatggcg cgcctgaagc tcgtgaaggt gccgcagctc ctcgtcgggg cctgcctcct
    10741 ctcggccttc ggcatgctgc tgctggcgag gtgaacggat gacgcacccg gtcgacatcc
    10801 tcctcgtcct cgtcatcctc ctgaacttcc tcgtcctcgg gacgccgtcg ctccggatga
    10861 cgatccgcgg cgcggccctc cagggggtcc tcctcgccct cctcccgctc ctcgtccacc
    10921 gcggcttcgg gtggcgggtc ggggcgatcg cggtcgccac gatggccctg aaggggctcc
    10981 tcatcccggg gctcctcgag aaggcgatgc gcgacgtcca catccgcagg gaggtcgagc
    11041 cgtacgtcgg cttcgtgccg tcgctcttcc tctgcgccgt cgggacggcc ctcgcgatcc
    11101 tcttctccgg gaacctcccg ctcgcccccg agcacgcggg gacgctcctc gtcccggcct
    11161 cgctcgcgac gctcctctcc ggcttcctgg tcctgacgac gcggaagaag gcgatctcgc
    11221 aggtcgtcgg ctacctgatc ctggagaacg gcatcttcat cttcggccag ctcctgatcg
    11281 aggcgatgcc gttcctcgtc gaggcggggg tcctcctcga cctcttcgtc ggcatcttca
    11341 tcatggggat cgtcatcaac cacatccgcc aggagttctc gtcgctcgac accgagctcc
    11401 tctcggagct gaaggactga gggggaggcg cgcatggaag tcggcctcgt cctcctcccg
    11461 ctcctcggcg ccgtcgtcgc ggccctcgtc cgctcgagag agcggcgggc ctgggtcgtc
    11521 ccggccgccg gggtcctgca cctcctcctc ctcggcggca tggtcctcgg gaacgtccgc
    11581 tcgggccgcg gcttctggct ccgcctcgac ccgctggcga cagtcatcct cgggacgatc
    11641 tcggtcctct tcgtcgtcgc ggccttctac ctgccgggct acctcgccct ccgccgcgac
    11701 cgcgacgacc gggtgttctg cgcggggctc ctcgtctttc tcgcgatgac ctcgctcctc
    11761 gcgctcgcgc agcacctcgg cctcctctgg gtcgcgatgg aggcgacgac gctcgcgacg
    11821 gcgccgctcc tctacttcaa ccgaacggcc cgctcgctcg aggcgacctg gaagtacctg
    11881 atgatcgggt cggtcggcat cgcgatcgcg ctcctcggct cgctcttcct cgcctactcg
    11941 gcccacgtcg gcggcgccga gccgtcgctc ctcttcgacg acctcctcgc gggcgcgggc
    12001 tccttctcgc gcccgtggct ccgggccgcg ttcgtcctcc tcctcgtcgg ctacgggacg
    12061 aagatgggcc tcgcgccgct ccacacctgg aagcccgacg cctacggcga ggcctccggc
    12121 atcgtcgggg cgctcctcgc gggcggaatg acgaacgccg ctttcctcgc cctcctccgc
    12181 gtctaccgcg tcgtgaacgc ggcgggggag ggggccttcg cccgggagct cctcgtcttc
    12241 atggggctcg tctcgatcgc gctcgccgcc gtcttcgtcg tgaggcagcg cgacctgaag
    12301 cggatgctcg cctactcgag cgtcgagcac gtcggcatcc tcgtcctcgg catcggcctc
    12361 ggcgggatcg gcgtcttcgg gtcgctgctg cacgcgatca acaacggcct gacgaagggg
    12421 gtcctcttcc tctccgtcgg gaacatccac cgcgccttcg ggagcaagca cctcgacgag
    12481 gtctcgggcg ccatctcccg cctcccggtc tcgggaagcc tcttcctcgt cggcttcttc
    12541 gcgatcaccg gctctccgcc gttcgggccg ttcgtctcgg agttcacgat cgtgaacgcc
    12601 gcgatcggcg gcggccacgc cgtcgccggg acgctcctcc tcgtcctcct cggca
//
LOCUS       DAOTEZ010000075        12400 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3579_length_12400_cov_5.004941, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000075 DAOTEZ010000000
VERSION     DAOTEZ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12400
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3579_length_12400_cov_5.004941"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2357
                     /gene="priA"
                     /locus_tag="P5164_05850"
     CDS             <1..2357
                     /gene="priA"
                     /locus_tag="P5164_05850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRY43452.1"
                     /translation="GLATSLLPSELPLPPSGARVAVPFGPRLLAGVVVPGEPTPPPDG
                     TVLREVLTVLDAEPFLPEAFVGLLTRAADYYFAPPGELLRAAVPARLLAAGTAAYVPT
                     SRAVGARAPEGLAGDVLSVVLASGRATALELAERVGSRGLGKALRTLLDAGLVRIPSE
                     ELRAARPPTAKSFAARGSADDLRLARRPGQRKALATLAALGRPASAAELRAAGVSAGT
                     LAALAKASLVTVVEEERPADAAAHALPARATAPIEPTPAQRAAFQAVAEALERGGEAR
                     FLLDGVTGSGKTEVYLAALEKAIALGRQGILLVPEIALAPGLIRRVVARFGSRVALLH
                     SGLSDGERSAAWERARRGDVDAVVGPRSAVFAPLPRLGLIVVDEAHDGSFKQAEAPRY
                     DSRTVARVRAHGEGAVLVLGTATPSMEDEQAARDGRLPRLVLPARPGSRERARVEVVD
                     LRGEAARPGDHGRVLFAARTVELLRGAFERGEQAIVLLNRRGFSPALLCRSCGHDFRC
                     ASCSVARTYHRRGERLLCHYCGDFVPRPRACPACGSDVLMPVGFGTERLAERFEELFP
                     EIAYAVLDRDAAARRGGAVRVLADFESGVAQALLGTQMVAKGHDFPNATVLAVLDADA
                     LLSFPDFRSAERTFQLVTQAAGRVGRGERPGVVAVQTARPEHEAIAAAVAQDHASFAE
                     GELRFRRAFGYPPFCHLLLALWTSKELPEAEAAARAGRAAIAGLPKLRLLGPAPAPLE
                     RLKGLYRVQLLVRSDSRDALAAAGELLRSLPEPPRLDRDPQSLL"
     gene            2392..3354
                     /locus_tag="P5164_05855"
     CDS             2392..3354
                     /locus_tag="P5164_05855"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HRY43453.1"
                     /translation="MSSDHRFEEPLERLRARIDGLRESPGSPARDKAIRKISDRLVKL
                     SGEIYANLTPWQKTLVARHPARPFTLDYVRVLLRDFVELRGDRAFADDPAIVAGFGVL
                     GGRTVAVVGHQKGRDTKEKVRRNFGMPRPEGYRKALRVMKLAEKFHRPVLTFIDTPGA
                     YPGVESEERGVSEAIAVNLLEMARLRVPLIATVTGEGGSGGALGIGVTDAILMLEHSV
                     YSVISPEGCAAILWKDQAKAREAAEAMRMTAADCKALGIADEVIPEPPGGAHSDPVAT
                     IDAVGQALVRHLDRLAAIPVDALLEARYAKFRRMGAWEGSAASR"
     gene            3351..5111
                     /gene="recJ"
                     /locus_tag="P5164_05860"
     CDS             3351..5111
                     /gene="recJ"
                     /locus_tag="P5164_05860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRY43454.1"
                     /translation="MTSAFPPPRVTRRLRPHDAGAAALLVRELGVAAPLARVLAARGF
                     VSPPEARAWLRPDPASLHDPFGMGGMDAAVDRLVDACRRGARVVVFGDYDCDGIGALA
                     ILTTTLKRLGADAVPFLPHRLRDGYGLRSETLRRVLDEHAPEGIVTVDCGITAVEPIR
                     EAVERGAFVIVTDHHLPPEELPRGAVLLNPRLPGCAYPFKELAGAGIAWKLSEALLLS
                     DGARVGLDAKAVRPWMASLAKVAALSTVADMVPLTGENRVLVSWGLAGLAEPRSPGLT
                     ALLSRSSVRAGKSPSVHDVAFRIAPRLNASGRIDHAFRALELLTTSDAARAEQLAEEI
                     EAANTERRRMQERVVAAALGRLEKTFEPDRDALVVETGTPEEGWHRGVLGIAASKVAQ
                     TVFRPVLLLSREDGRVGGSGRTWGRTPLHERLAPVARRHEAEFGGHHAAIGISVAASA
                     FEAFRNDAKEAFARSRDEEEWSVVLEADAPLRAAEVTPELVEHLGRLEPHGMGNPKPL
                     FLLRSLSWNGRGRPVGEHGLRLVLEGEGVRLDAVGWSLGRIPPAGRAGTWDVLANIAL
                     DAFLGRPALTVLDATPAEAA"
     gene            5108..6466
                     /locus_tag="P5164_05865"
     CDS             5108..6466
                     /locus_tag="P5164_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43455.1"
                     /translation="MRPVARRRLRRALGLLAFAGFPLAIVFSWRPAGARTDSAPAEVA
                     ETLLAESAGIRDRVRFEEFDYSETEKDSEVYRLRAAEAIAFADGEDRTFRLKDVTFVT
                     RDAGSGRAAVLEAPRAEFVPSTKAFRVFEGVRVEGAGIHLTGRSFRYDPQTRLLVSDG
                     PVTAIRGALVARALGGAVESGAGIVRFRDDVRLGGEDDRGRRIALTADAVDLEKGGGL
                     AARGHVVLKTDDLVLRGREVERRPAGDGDRVTARGGVEALLVRGAGGELDSPIRASGE
                     HLELERNAEAEPSRLGLSGAPARIDLPPAEGTGARRAISRDFEARLADGRLDRVQVPS
                     AFRLEESAPPAVPGSTRAPASRLLEAGRGQLAFGSDGRTLETAVLEGGVALSEGERTR
                     VTAPSATLRAEDESAVFAGSGPERARYEDETARVSAEVLTWFRREQRMEGVGDVKASF
                     RG"
     gene            6482..7117
                     /locus_tag="P5164_05870"
     CDS             6482..7117
                     /locus_tag="P5164_05870"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HRY43456.1"
                     /translation="MGGDADTPVFSESDFLRVRPEERTVLLTGNVTAWQEENVLRGQS
                     ILLDDRDRSLRAERDVRAVLRRRRIDPTTKAATVETITASGSVLTHRESDRLIRIEGS
                     ANVVSGTWTMKADVTDVVLGPERTVDHAESRGNVVVEDRADGRRGEGAKATWDPRADA
                     VALEGRPATALDGKGNRMTGARLRFQRGSGRVDVETGPGIPSQGVLRPEGT"
     gene            7114..7851
                     /gene="lptB"
                     /locus_tag="P5164_05875"
     CDS             7114..7851
                     /gene="lptB"
                     /locus_tag="P5164_05875"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRY43457.1"
                     /translation="MSETRPRLAAEALRKSYRGRTVVDGVSIEVNGGEVVGLLGPNGA
                     GKTTTFSIVVGLVEPDDGHVLLDGEEITALPMFRRARRGVGYLPQEASVFRRMTALQN
                     LVAILETLPLSRNERLERAHELLDRFKLSHLSASIADTLSGGERRRLEIARALTLSPR
                     FLLLDEPFAGIDPITVLDLQHVIRELATTGIGILITDHNVRDTLAIVDRGYIINAGRI
                     FRTGSPVSLSQDPEVRRVYLGDRFRLD"
     gene            7896..9503
                     /gene="rpoN"
                     /locus_tag="P5164_05880"
     CDS             7896..9503
                     /gene="rpoN"
                     /locus_tag="P5164_05880"
                     /inference="COORDINATES: protein motif:HMM:NF009118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRY43458.1"
                     /translation="MGLEQKLSLKLSQRLVMTPTLQQAIKLLQMTRLELHDVVNQEIV
                     ENPVLEEDEAVDVPAGTEADEAVAVREGAEAADASDPRERPAEGTEAGDAAALAWDDA
                     GGAAGEAAPPPPAVGTAEAEIPAEPTQESFEAIDLEAYFGDYLEGSASTAPRMDEEGE
                     EYSLENRAEAPPGLDAHLAEQLGVSEATPTVRAACEYLIGNLDADGYLRLPLDEVAGA
                     IPCSEAEAAEALGLLQSFDPLGVGARSLAECLLIQARAEGLATPLLEDLVTNRLHDLA
                     TKAVPVLARQMNVSAEELQQAIDVLKRLDPKPGRRYDSTRTIYVEPDVQVVKVDDDYV
                     VLFNDDGLPRLRVSPFYRRLLSSSNGPLDGEGRSYLREKMRAAQWLMKSLDQRKRTIV
                     RVAESIVRKQRDYLEWGVAHLKPLILRDVADDIGMHESTVSRVVSNKWMATPRGLLPM
                     KFFFHSAISSSAGEDVSSLAVKGKIKALVDAEDASQPLSDARLSELLAREGIRIARRT
                     VAKYREELHIPSSSIRKVERGPAGSPE"
     gene            9537..10070
                     /locus_tag="P5164_05885"
     CDS             9537..10070
                     /locus_tag="P5164_05885"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HRY43459.1"
                     /translation="MKIEVTARRLTVDAKTKDTIAKRLEKLSKVLPREAEAKVVVRLE
                     KRGVLVEVTITARQRTWAAEAVAADQLTAAQSALERVEAQAKKTKARVKDEKKHTTSG
                     VRSPEVSLPVEKAPAPREKAPRAESYEARRMFDEDALHAFDRSKREVLVYRDPSDEQL
                     RVLYRRKDGAVVILTPR"
     gene            10078..11097
                     /gene="hprK"
                     /locus_tag="P5164_05890"
     CDS             10078..11097
                     /gene="hprK"
                     /locus_tag="P5164_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HRY43460.1"
                     /translation="MLPTPPETKPHVRAADLESPALAPLGLKLVSGRAGLDRPIEWPR
                     VQKPGLAIAGFLPYVRAQRVQILGESEFDYLKTLSPRVVKQRFDAFTSLGMSCVVVTK
                     GIRPPAVLRRFCQERDVPLFATPRLTSTVIEGLTAFLEESLAPRVTLHGVLVEVGGLG
                     TLLLGDSGVGKSECALALVQRGHRLVADDLVVVKRFHNDALVGSSAGVIRHHMELRGL
                     GIVNVPLLFGVAAVLEKHTVELVIRLSHWVEGVEYDRLGLDEESETILGVPVPLVRMP
                     VATGRDVALLVEIAARNHLLKRRGYNAAREMADRVHEEIARRTGSTDRERRRGRGRPR
                     HGGGA"
     gene            11094..11975
                     /gene="rapZ"
                     /locus_tag="P5164_05895"
     CDS             11094..11975
                     /gene="rapZ"
                     /locus_tag="P5164_05895"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HRY43461.1"
                     /translation="MTGARDDQRLLVVTGLSGSGKSYAARALEDIGYNTIDNLPLALL
                     RSFVEEAARGTGGPGRNAVVLDVRNPGFAEAFPPLLDSLRPILPVTVVFLEADEPTIF
                     RRFSETRRPHPLALDRTLPEAMATERKLLAEVKGKADLVLDTTAMTVHELRSVLAEHF
                     RSPGDPGVLAVSILSFGFKHGVPPSIDLCFDVRFLANPHFVPELRHRTGRDPNVARFI
                     EEGTATGPFYGRLLDFLLFCLPNYRRENRAYLTIGVGCTGGRHRSVYVAERLGRDLAA
                     AGYPVRVSHRDDNREGP"
     gene            11972..>12400
                     /locus_tag="P5164_05900"
     CDS             11972..>12400
                     /locus_tag="P5164_05900"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRY43462.1"
                     /translation="MKNPEASVAGAEDSVGILLLSHGPLADALRETVQVLEPQPGEPL
                     GALSLAWDEAPEHASERLQRAIADADRGRGVVLLTDMFGGTPSNLALAFLQKGRVEIV
                     TGVNLPMVVKARALAREGKEPGEMARTLVEKGRRSIVAAGE"
BASE COUNT         1689 a         4545 c         4477 g         1689 t
ORIGIN      
        1 cgggactcgc cacgtccctg ctcccgtccg agctgccgct ccctccgtcg ggcgcgcgcg
       61 tcgccgtgcc gttcgggccg cgtctgctgg cgggtgtcgt cgtcccgggg gagcccaccc
      121 cgccgcccga cggcacggtc cttcgcgagg tcctcacggt cctcgacgcc gagccgttcc
      181 tcccggaggc gttcgtcggc ctcctgaccc gggccgccga ctactacttc gctccgcccg
      241 gcgagctcct gcgagcggcg gttcccgccc ggctgctcgc ggccgggaca gcggcctacg
      301 tcccgacgag ccgcgccgtc ggcgcgcgcg cgccggaagg gctcgcgggg gacgttctct
      361 ccgtggtcct ggcttcgggg cgcgcgacgg cgctcgagct cgctgagcga gtcggctccc
      421 gcggcctcgg gaaagccctt cgcacgcttc tcgatgccgg cctcgtccgg attccctcgg
      481 aggagctccg ggccgcacgc cccccgaccg cgaagagctt cgcggcgcgg gggagcgcgg
      541 acgacctccg cctcgctcgg cgtcccggac agaggaaggc cctcgccacg ctcgccgcgc
      601 tcgggagacc ggcgtcggcg gcggagcttc gggccgcagg ggtctccgcg gggacgcttg
      661 ccgcgctcgc gaaggcgtcg ctcgtcacgg tggtcgagga ggagcggccg gcggacgccg
      721 ccgcccacgc gcttccggcg cgggccacgg cgcccataga gccgacgccc gcgcagcgtg
      781 ccgccttcca ggccgtggcg gaagcgctcg aacgcggcgg cgaggcccgc ttcctcctcg
      841 acggcgtcac cggcagcgga aagacggagg tctacctcgc cgcgctcgag aaggcgatcg
      901 ccctcggccg gcagggaatc ctcctcgtgc ccgagatcgc cctcgcgccc gggctcatcc
      961 ggcgggtcgt cgcgcggttc ggctcgcgcg tcgctctgct ccacagcggc ctttcggacg
     1021 gagagcgctc cgccgcctgg gagcgggcgc gacgcggcga cgtggacgcc gtggtcggcc
     1081 ctcgctccgc cgtcttcgct ccgctgcccc ggctcgggct gatcgtcgtc gacgaggcgc
     1141 acgacggctc cttcaagcag gccgaggcgc cgcggtacga ctcccggacc gtggcccggg
     1201 ttcgggcgca cggcgaaggg gcggtgctcg tcctcgggac ggcgacgccg tccatggagg
     1261 acgagcaggc cgcccgagac ggacgccttc cgcgcctcgt ccttccggcg cgtcccggct
     1321 cccgcgagcg ggcccgcgtg gaagtcgtcg acctccgcgg cgaggccgcc cgtcccggcg
     1381 accatggccg cgtcctcttc gctgcccgga cggtggagct cctccgcggg gcattcgagc
     1441 gcggcgagca ggcgatcgtc ctcctgaacc gccgcggctt ctcgccggcg ctcctctgcc
     1501 ggtcctgcgg ccacgacttc cgctgcgcct cctgctccgt cgcccggacc tatcaccggc
     1561 gcggggagag gctcctctgc cattactgcg gcgacttcgt ccccaggccg cgcgcatgcc
     1621 ccgcatgcgg ctccgacgtc ctgatgccgg tcgggttcgg caccgaacga ctcgccgagc
     1681 ggttcgagga gctctttccg gagatcgcgt atgcggtcct cgaccgggac gccgccgcgc
     1741 gccgcggcgg agcggtccgc gtcctcgccg acttcgagtc gggcgtcgcg caggcgctcc
     1801 tcggcacgca gatggtggcg aaggggcacg acttcccgaa tgccacggtc ctcgccgtcc
     1861 tggacgccga cgctcttctc tcgttccccg actttcgctc cgcggagcgg accttccagc
     1921 tcgtgaccca ggcggccggc cgggtcggcc ggggcgagcg gccgggagtc gtggccgtcc
     1981 agacggcccg gcctgagcac gaagcgatcg cggccgccgt cgcccaggac cacgcctctt
     2041 tcgcggaggg cgagctccgg ttccggcgcg ccttcggcta cccgccgttc tgccacctcc
     2101 tcctcgcgct ctggacgtcg aaggagctcc cggaggccga agcggccgcg cgtgccggcc
     2161 gggcggcgat cgcggggctg ccgaagctcc ggctgctcgg accggcgccg gcgccgctgg
     2221 agcggctcaa ggggctctac cgggtccagc tcctcgtccg gtccgactcc cgagatgcgc
     2281 tggcggcggc cggcgagctc ctccggtcgc tcccggagcc gccccggctg gaccgggacc
     2341 cgcagagcct cctctgacgg tccggccgtc gcggtctggt acattccggg ggtgagttcc
     2401 gatcaccggt tcgaggagcc gctggagcgg ctccgagcgc gcatcgacgg cctgcgggag
     2461 agccccggga gccccgcccg ggacaaggcg atccgcaaga tctccgacag gctcgtgaag
     2521 ctctcggggg agatctacgc gaacctcact ccctggcaga agacgctcgt cgcgcgccac
     2581 ccggcccgcc cgttcacgct cgactacgtc cgggtcctcc tgcgcgactt cgtcgagctg
     2641 cgcggcgacc gggccttcgc ggacgacccc gcgatcgtgg ccggcttcgg agtcctcggc
     2701 ggccggacgg tcgccgtcgt cggccaccag aaggggcgcg acacgaagga gaaggtccgt
     2761 cggaacttcg gcatgccccg ccccgagggc taccggaagg cgcttcgcgt catgaagctc
     2821 gccgagaagt tccatcggcc cgtcctgacg ttcatcgaca cgccgggcgc gtatccgggg
     2881 gtcgagagcg aggagcgagg agtctcggag gcaatcgccg tgaacctcct cgagatggcg
     2941 cgcctccggg tgcccctgat cgcgaccgtg acgggcgaag gcggatcggg aggcgctctc
     3001 gggatcggcg tgaccgacgc gatcctgatg ctcgagcact cggtctactc cgtcatctcg
     3061 ccggaagggt gcgccgcgat cctctggaag gaccaggcga aggcgcgcga ggcggcggag
     3121 gcgatgagga tgaccgccgc cgactgcaag gccctcggga tcgcggacga ggtgatcccg
     3181 gagcccccgg gcggcgccca ctccgacccg gtggcgacga tcgacgcggt gggtcaggcg
     3241 ctcgtccgcc acctcgaccg cctcgcggca atcccggtcg acgccctcct cgaggcccgc
     3301 tacgcgaaat tccgccgaat gggggcctgg gaggggtccg cggcgtcccg gtgacctcgg
     3361 ccttcccgcc gccacgcgtc acacgcagac tccggcctca cgacgcgggc gcggccgccc
     3421 tcctcgtgcg ggagctgggg gtcgccgcgc ctctcgcccg ggtcctcgcc gcgcggggtt
     3481 tcgtctcgcc acccgaggcg cgggcctggc tccggcccga cccggcgtcg ctccatgacc
     3541 ccttcggcat gggaggcatg gacgcggccg tcgaccgcct cgtcgacgcc tgccggcgtg
     3601 gtgcgcgggt cgtcgtcttc ggcgactacg actgcgacgg gatcggcgcg ctcgcgatcc
     3661 tgacgacgac gctcaagcgg ctcggggccg acgcagtgcc gttcctcccg caccggctcc
     3721 gggacggcta cggtctccgc tccgagacgc ttcgccgggt cctcgacgag cacgcgccgg
     3781 aagggatcgt cacggtcgac tgcggcatca cggcggtgga gccgatccgg gaggcggtgg
     3841 agcgcggcgc gttcgtcatc gtcacggacc accacctgcc cccggaagag ctgccgaggg
     3901 gcgccgtcct cctgaacccg cgactgccgg gctgcgcgta cccgttcaag gagctggcgg
     3961 gggcggggat cgcctggaag ctctccgagg cgctcctcct ctcggacggc gcccgcgtcg
     4021 gcctggacgc gaaggccgtc cggccctgga tggcctcgct cgcaaaggtc gcagcgctct
     4081 cgacggtcgc cgacatggtc cccctcacgg gagagaaccg ggtcctcgtc tcctgggggc
     4141 tcgcgggtct cgccgagccg cgctcgccgg gcctgaccgc gctcctttcg cgctcctcgg
     4201 tccgagccgg gaagagcccc tccgtccacg acgtggcgtt ccggatcgcc ccgcgcctga
     4261 acgcgtccgg ccggatcgac catgcctttc gcgcgctcga gctgctgacg acgtccgacg
     4321 ccgcccgagc cgagcagctg gccgaggaga tcgaggcggc gaacacggag cggcggcgca
     4381 tgcaggagcg ggtcgtggcc gccgccctcg ggaggctcga gaagacgttc gaacccgatc
     4441 gcgacgctct cgtcgtggag accggaacgc cggaagaagg gtggcaccgc ggggtcctcg
     4501 gcatcgccgc ctcgaaggtc gcccagaccg tcttccgccc cgtgcttctc ctctcccggg
     4561 aggacgggcg ggtcggcggg agcggccgga cctggggccg gaccccgctc cacgaacggc
     4621 tcgcgcccgt cgcccggagg cacgaggcgg agttcggagg acatcacgcg gcgatcggga
     4681 tttccgtggc cgcgagcgcc ttcgaggcct tccggaacga cgcgaaggag gccttcgccc
     4741 ggagccgcga cgaggaggag tggtccgtcg tcctcgaagc cgacgctccg ctccgcgccg
     4801 cggaggtgac gccggagctc gtcgagcacc ttgggcgcct cgagcctcac gggatgggga
     4861 acccgaagcc gctcttcctc ctccgcagcc tctcctggaa cggtcgcggt cgccccgtcg
     4921 gggagcacgg cctccgactc gttctggagg gagaaggagt ccggctggac gcggtcggct
     4981 ggtccctcgg caggatccct cccgccggga gggccgggac ctgggacgtc ctcgcgaaca
     5041 tcgctctcga cgcgttcctg ggccgaccgg cgctcaccgt cctcgacgcg accccggcgg
     5101 aggcggcatg aggccggtcg cgcggcgccg gcttcgacgg gcgctcgggc tgctggcgtt
     5161 cgcgggcttt cccctcgcca tcgtcttctc gtggcggccc gccggcgcgc gcacggactc
     5221 tgcgccggcc gaggtggccg agacgctcct cgcggagtcg gcggggatcc gcgaccgggt
     5281 gaggttcgag gagttcgact actcggagac ggagaaggac tccgaggtct accgcctccg
     5341 ggccgccgag gcgatcgcat tcgccgacgg tgaggaccgg acctttcgcc tgaaggacgt
     5401 caccttcgtg acgcgagacg ccgggtcggg ccgcgcggcc gtcctcgagg cgccgcgcgc
     5461 ggagttcgtc ccctcgacga aggcgttccg cgtcttcgag ggcgtccggg tcgagggagc
     5521 gggaatccac ctgaccgggc gctcgttccg ctacgacccg cagacgcgcc tcctcgtctc
     5581 cgacggtccg gtcacggcga ttcgcggcgc gctggtcgcg cgcgcgctcg gcggcgcggt
     5641 cgagtcgggg gccgggatcg tccgcttccg ggacgacgtt cggctcgggg gcgaggacga
     5701 caggggccgc cgaatcgcgc tgacggcgga tgccgtcgat ctcgagaagg gagggggcct
     5761 cgccgcacgc ggacacgtgg tcctgaagac ggacgacctc gtcctcaggg gacgcgaggt
     5821 cgagcgtcgc ccggccggag acggggaccg ggtcacggcc cgaggcggcg tcgaggcgct
     5881 cctcgtccgc ggagccggcg gagagctcga ctctccgata cgggcgtccg gcgagcacct
     5941 cgagctggag cggaacgccg aggccgagcc gtcccggctg ggcctctcgg gagctccggc
     6001 gcggatcgac ctccctcccg ccgaggggac gggcgcccgg cgcgcgatct cacgggactt
     6061 cgaggcgagg ctcgcggacg gaaggctcga ccgggtccag gtcccctccg cgtttcggct
     6121 cgaggagtcg gctccgccgg cggttccggg ctccacgcgg gccccggcgt cccgccttct
     6181 cgaggccggt cgcggacagc tcgcgttcgg gtccgacggc aggacgctcg agacggccgt
     6241 cctcgagggg ggcgtcgctc tgagcgaagg ggagaggacg agggtgaccg ctccgtcggc
     6301 gaccctacgc gccgaggacg agtccgccgt cttcgcggga agcggcccgg aacgggcgcg
     6361 ttacgaggac gagaccgctc gcgtctccgc ggaggtcctg acctggttcc gccgcgagca
     6421 gcggatggag ggagtcggcg acgtgaaggc gtccttccgg gggtgaaagg gttccgagat
     6481 cctgggcggc gacgcggaca ccccggtctt ctccgagtcg gacttccttc gggtccgccc
     6541 cgaggagcgg acggttctcc tcacggggaa cgtgaccgcc tggcaggagg agaacgtcct
     6601 tcgcgggcag tcgatcctcc tcgacgaccg ggaccggtct ctccgggccg aaagggacgt
     6661 gcgcgccgtc ctgcggagac gccgcatcga tccgacgacg aaggcggcga ccgtcgagac
     6721 gatcacggca tcgggaagcg tcctcacgca ccgcgaatcg gaccggctca ttcggatcga
     6781 gggatcggcg aacgtcgtct ccgggacctg gacgatgaag gccgacgtga ccgacgtcgt
     6841 cctcggcccc gagcgaacgg tcgaccacgc ggagtcccgc gggaacgtcg tcgtcgagga
     6901 ccgggccgac ggccgccgcg gcgaaggggc gaaggcgacc tgggaccccc gcgccgacgc
     6961 cgtcgcgctc gaaggccggc cggcgacggc gctcgacggg aagggaaacc gaatgaccgg
     7021 cgcgcggctc cgcttccagc gcgggagcgg ccgcgtcgac gtggagacgg gtccgggaat
     7081 ccccagccag ggggtcctcc ggccggaggg aacgtgagcg agacacgacc gcgcctcgcc
     7141 gcggaagcgc tcaggaagag ctaccggggg cgcaccgtcg tcgacggcgt ctccatcgag
     7201 gtgaacggag gcgaggtcgt cggcctcctc gggccgaacg gagccgggaa gacgacgacg
     7261 ttctcgatcg tcgtcggcct cgtcgagccc gacgacgggc acgtcctcct cgacggcgag
     7321 gagatcaccg cccttccgat gttccgcagg gcccggcgcg gcgtcggcta cctcccgcag
     7381 gaagcgtccg tctttcgcag gatgaccgcg ctccagaatc tcgtggcgat cctcgagacg
     7441 ctgccgctct cgcggaacga aaggctcgag cgggcgcacg agctgctcga ccgtttcaag
     7501 ctctctcacc tctccgcctc gatcgccgac acgctctccg gcggcgagcg gcggcggctc
     7561 gagatcgcgc gggccctgac gctctctccg cggttcctcc tcctggacga gccgttcgcg
     7621 gggatcgacc cgatcaccgt cctcgacctg cagcacgtca tccgcgagct cgccacgacc
     7681 ggaatcggca tcctcatcac cgaccacaac gtccgggaca cgctcgcgat cgtcgaccgc
     7741 ggctacatca tcaacgccgg ccggatcttc cggaccggct ctcccgtctc gctttcccag
     7801 gaccccgagg tgcggcgcgt ctacctcggg gaccgcttcc ggctggacta gaatcgcgga
     7861 aggaagcgcg ccgtccggcg cgaccggctc acacagtggg actcgaacag aagctctccc
     7921 tcaagctctc gcagcggctc gtcatgacgc cgacgctgca gcaggccatc aagctgctgc
     7981 agatgacgcg tctcgagctg cacgacgtcg tcaaccagga gatcgtggag aacccggtcc
     8041 tggaggagga cgaggccgtg gacgtcccgg cgggcacgga ggccgacgag gcggtcgcgg
     8101 tgagggaagg ggccgaggcg gcggatgcgt ccgacccgcg cgagcgcccc gccgagggga
     8161 ccgaggcggg ggacgcggcc gcgctggcct gggacgacgc gggaggggcc gccggagagg
     8221 ccgccccgcc acctccggcg gtcgggacgg cggaagccga gatcccggcg gagccgacgc
     8281 aggagtcgtt cgaggcgatc gacctcgagg cctacttcgg cgactacctc gaggggagcg
     8341 cgtcgaccgc accgcgcatg gacgaggaag gcgaggagta ttcgctcgag aaccgggcgg
     8401 aggccccgcc gggcctcgac gcccatctcg ccgaacagct cggagtctcc gaagcgacgc
     8461 ccacggtccg ggccgcctgc gagtacctca tcgggaacct cgacgcggac ggctacctga
     8521 ggcttcctct cgacgaggtc gccggcgcga tcccgtgctc ggaagccgag gcggccgagg
     8581 ccctcgggct ccttcagtcg ttcgacccgc tcggagtcgg ggcgcgcagc ctcgccgagt
     8641 gcctgctgat ccaggcccgc gccgagggcc tcgcgacccc tctcctcgag gacctcgtca
     8701 cgaacaggct ccacgacctc gcgacgaagg cggtcccggt cctcgcgcgc cagatgaacg
     8761 tgtcggccga ggagctgcag caggcgatcg acgtcctcaa gcgcctcgac ccgaagcccg
     8821 gccggcggta cgactcgacc cggacgatct acgtcgagcc cgacgtccag gtggtgaagg
     8881 tcgacgacga ctacgtcgtc ctgttcaacg acgacggcct gccccggctc cgcgtgtcgc
     8941 cgttctaccg gcgcctcctc tcctcgagca acgggccgct cgacggggag gggcggagct
     9001 acctccgcga gaagatgagg gcagcccagt ggctcatgaa gagcctcgac cagcggaagc
     9061 ggacgatcgt ccgcgtcgcc gagtcgatcg tacggaagca gcgcgactac ctcgagtggg
     9121 gcgtcgcgca cctcaagccg ctgatcctcc gcgacgtcgc cgacgacatc ggcatgcacg
     9181 agtcgaccgt ctcccgggtc gtctcaaaca agtggatggc gaccccgaga ggcctgcttc
     9241 cgatgaagtt cttcttccac tcggcgatca gctcctcggc gggcgaggac gtctcctcgc
     9301 tcgccgtaaa ggggaagatc aaggcgctgg tcgacgccga ggacgcgtcg cagccgctct
     9361 ccgacgcgcg cctctccgag ctcctcgcgc gcgaggggat ccgcatcgcc cgccggacgg
     9421 tggcgaagta ccgcgaggag ctgcacattc cctccagctc gatacggaaa gtggagcgcg
     9481 ggccggccgg gtcgccggaa tgaggcccga cgcctgatga ccatcgagga ggcacgatga
     9541 aaatcgaagt caccgcccgg cgtctcaccg tcgacgcgaa gacgaaggac acgatcgcca
     9601 agcgcctcga gaagctctcg aaggtcctcc cgcgggaggc ggaggcgaag gtcgtcgtcc
     9661 gcctcgagaa gcgcggggtg ctcgtcgagg tgacgatcac cgcgcgccag cggacgtggg
     9721 cggccgaggc ggtcgccgca gaccagctga cggccgcgca gagcgcgctc gagcgggtcg
     9781 aggcgcaggc gaagaagacg aaggcccggg tcaaggacga gaagaagcac acgacgtccg
     9841 gcgtgcggag cccggaggtc tcgctccccg tcgagaaggc gcccgctcct cgcgagaagg
     9901 ccccccgggc cgagagctac gaggcgcggc ggatgttcga cgaggacgcc ctccacgcct
     9961 tcgaccgcag caagcgcgag gtcctcgtct acagggatcc gtccgacgag cagctccgcg
    10021 tcctctaccg ccgaaaggac ggcgccgtcg tcatcctgac gccgcgctag gcgctcgatg
    10081 ctcccgaccc ccccggagac gaagccgcac gtccgcgcgg ccgacctcga gtcgcccgcc
    10141 ctggcgccgc tcgggctcaa gctcgtctcc gggcgggccg ggctggaccg gccgatcgag
    10201 tggccccggg tccagaagcc gggactcgcg atcgccgggt tcctgccgta cgtgagggcg
    10261 cagagggtgc agatcctggg ggagagcgag ttcgactacc tgaagacgct ctcgcccagg
    10321 gtcgtgaagc agcggttcga cgcgttcacc tcgctcggaa tgtcgtgcgt cgtcgtcacg
    10381 aaggggatcc ggccgcccgc cgtcctccgc cgattctgcc aggagcggga cgtcccgctc
    10441 ttcgcgacgc cgcggctgac gagcacggtg atcgaggggc tcacggcgtt cctcgaggag
    10501 tcgctcgcgc cgcgcgtgac gcttcacggc gtcctcgtgg aggtgggtgg gctcggtacg
    10561 ctcctgctcg gcgactccgg cgtcggcaag agcgagtgcg ccctggccct cgtccagcgc
    10621 ggtcatcggc tcgtcgcgga cgacctcgtc gtcgtcaaga ggttccacaa cgacgccctc
    10681 gtcggctcgt cggcgggcgt catccgccac cacatggagc tgcgggggct cggcatcgtg
    10741 aacgtcccgc tcctcttcgg agtcgccgcg gtcctcgaga agcacacggt cgagctcgtg
    10801 atccgcctct cgcactgggt cgagggggtc gagtacgacc gtctcggcct cgacgaggag
    10861 tccgagacga tcctcggcgt gccggtcccc ctcgtccgga tgcctgtggc gaccgggagg
    10921 gacgtcgcgc tcctcgtcga gatcgccgcc cggaaccacc tcctgaagag gaggggctac
    10981 aacgcggcgc gcgagatggc ggaccgggtc cacgaagaga tcgcccgccg gaccggttcg
    11041 accgacaggg agcgccggcg cggccgcggg cggcctcgcc acgggggtgg cgcgtgacgg
    11101 gcgcgcggga cgaccagcgt ctcctcgtcg tgaccgggct ctccggctcg ggaaagtcgt
    11161 acgcggcgcg ggccctcgag gacatcggct acaacaccat cgacaacctc ccgctcgcgc
    11221 tcctccggag cttcgtcgag gaggcggccc gcgggacggg cggtcccggc cgcaacgcgg
    11281 tcgtcctcga cgtgaggaac cccggattcg ccgaggcgtt cccgcccctc ctcgactcct
    11341 tgcggccgat cctcccggtc acggtcgtct ttctcgaggc cgacgagccg accatctttc
    11401 ggcgcttctc ggagacccgc cgtccgcatc cgctcgcgct ggaccggacg ctgcccgagg
    11461 cgatggcgac ggagcggaag ctcctggccg aggtgaaggg gaaggccgac ctcgttctcg
    11521 acacgacggc catgaccgtc cacgagctcc ggtcggtcct cgccgagcat ttccggtccc
    11581 ccggtgaccc cggcgtcctc gccgtatcga tcctctcatt cgggttcaag cacggggtcc
    11641 cgccctcgat cgacctctgt ttcgacgtga ggttcctcgc gaacccgcac ttcgtgcccg
    11701 agctccggca ccggaccggg cgcgacccga acgtcgcccg attcatcgag gaggggaccg
    11761 ccacgggccc gttctacggc cggctcctcg acttcctcct cttctgcctt ccgaactatc
    11821 ggcgcgagaa ccgggcctac ctgacgatcg gcgtcgggtg cacgggaggc cgccaccgct
    11881 ccgtctacgt cgcggagcgc ctcggccgcg acctcgccgc ggccgggtac cccgtccgcg
    11941 tctcgcatcg cgacgacaac cgggaaggac catgaagaac cccgaagcct ccgtcgccgg
    12001 cgctgaagac tccgtcggca tcctcctcct ttcccatggc ccgctggccg acgcgctgcg
    12061 cgagaccgtc caggtcctgg agccgcagcc gggcgagccg ctgggcgccc tctcgctcgc
    12121 ctgggacgaa gcgccggagc acgcgagcga gcgactccag cgggcaatcg ccgacgccga
    12181 ccgcgggcgc ggcgtcgtgc tgctgaccga catgttcggc gggacgccct cgaacctggc
    12241 gctcgcgttc ctccagaagg ggcgcgtcga gatcgtgacc ggggtgaacc tgccgatggt
    12301 ggtgaaggcg cgggccctcg cgcgggaggg gaaggagccg ggcgagatgg cgcggaccct
    12361 cgtggagaag gggcggcgct ccatagtcgc cgcaggcgag
//
LOCUS       DAOTEZ010000076        12272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3623_length_12272_cov_5.804289, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000076 DAOTEZ010000000
VERSION     DAOTEZ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12272
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3623_length_12272_cov_5.804289"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="P5164_05905"
     CDS             complement(<1..236)
                     /locus_tag="P5164_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43463.1"
                     /translation="MTTAPDGSTVPASGMPASPAGLRTALERIAAPERVLDRPLDRIA
                     FASDASFYRLIPKAVVLTESVDEVAALFRLARET"
     gene            complement(307..1410)
                     /locus_tag="P5164_05910"
     CDS             complement(307..1410)
                     /locus_tag="P5164_05910"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRY43464.1"
                     /translation="MKLVLTLARAAAAAALAVAFLPSPAAAQAVIKVNDNVNFKLGFQ
                     FQGWADWTQSAATGGYAQNLFARRARLLVGGQVAKDVTFFFQTDSGNLGKVNPAGAKQ
                     TNTGFVIQDAFVSWKLNDALTLEAGHFLVPLSRNILQSTLSFFTLDISPASTVMAGPT
                     QTNGLRDNGFQAKGYLVDGGRLEYRAAVMQGVRDSASRNSFRTAGYLQYNFFETERGY
                     VFAGTNLGKKKVVNVSAGADLQKDYRAFSGDVFATIPVAKGDEVGGQVQWIRYDGGDF
                     LASIPKQDDLLVEAAYYLSSAKVQPFLKYESQSFANAADEGKDIERFGGGLNWYVSGQ
                     NFKLTAQVLRVEPKSSAVKPTNQFTVQLQAFYF"
     gene            complement(1419..3116)
                     /locus_tag="P5164_05915"
     CDS             complement(1419..3116)
                     /locus_tag="P5164_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005037532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HRY43465.1"
                     /translation="MTWTQIYTPVAGNLLLSALVAALPVVVLLGLLAFFHVKAHKAAL
                     LGLATSLGVAIFVYGMPTKLALTSALNGAAYGLFPIGWIVLCAIFVYDLTVKSGDFEV
                     VKHTVAGLADDRRIQALLIAFSFGAFIEGAAGFGTPVAISAAMLIGLGFRPLLAAGIA
                     LIGNTAPVAYGALGTPIVVLASVTGLDVQALSAMAGRQLPFFSLLVPFWLVWAMSGRK
                     AMWEVWPACLTSGLSFAITQFLVSNYHGPWVVDIAGAIVSMGATVLLLKFWQPKKTWT
                     FDHENHADRAAEAAARPVHTPKRALMAWMPWILLSVFVFAWGLPQVKDVLNGGKKDSP
                     NVLKGVSMLKWEVPGLHKAVIKAPPVVKEEKPEEAVFTFNWLSATGTSLLLSGILSGL
                     LLGFSLKEIAKVFWGTLMRVRISLLTIGAMLALGFSTRYAGLDATMGLAFASTGVLFP
                     FFSPLLGWLGVALTGSDTSSNVLFGGLQMISAEKLGINPVLAAAANSSGGVMGKMIDA
                     QSIVVASVATNQQGQEGTILRYVFWHSIVLACLVGVLVFLQAYVFPWMIPTVEAVAK"
     gene            complement(3270..3917)
                     /locus_tag="P5164_05920"
     CDS             complement(3270..3917)
                     /locus_tag="P5164_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY43466.1"
                     /translation="MSTTILLADDHRIVREGLRTLLSQQPEMEVVGEAADGREAVSQA
                     LLLRPGVVVMDIAMPELNGVEATRLILAELPGTRIVALSMYADRRFVAEVLRAGALGY
                     VLKDGAFEELALAIRTTLAGKTYLSPSIAGLVVEELVRKASTPGSPSLASLTPRERQV
                     LRLLAGGKRPREIAQELEISSKTVEVHKQNLMNKLEIHSAAELTRFAIREGLATP"
     gene            complement(3914..6241)
                     /locus_tag="P5164_05925"
     CDS             complement(3914..6241)
                     /locus_tag="P5164_05925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRY43467.1"
                     /translation="MRFRALLAALALLLGPAAGAVAAGAATSGADAASGHRVLVLHSY
                     YKGYKWADDEHRGITSVLEPVLGVENVFVEYMDTKRFYEPRHVAQLPEVYRRKYADHR
                     IDLVVGVDDRAVDFLVEHRDEVFPGTPVVFCGVNYFREEKLKGRRLFTGVSEDADLGA
                     SLETALDLHPGTRRIYVVNDDTETGLAVRARLAELGPAVRGVPMEPLDGLSMERLLAR
                     LRTLPPDSLVFFAFFFRDADGRVFGYDESARLVTGASSVPVYGAWDFNLGLGIVGGRL
                     TSGFDQGEAAARIALRILRGEAPESIPVLRGLPGRDLFDHLALARWGIPASRLPKGST
                     VLNLPESAFARYRKVFLAGAGALVLLGVLNAVLALNISRRKLAEEALRRHQEALEETI
                     RQRSEELSRANEELRRDILERARTERALRVAESNLRSIFENAAEGIYQSTPSGRFLTV
                     NSALAAMAGYASPAEMTESISDIRRDFYVDPSSRERFEEALVEEEEVRGFESLVRRKD
                     GALIWVLENARAVRDEKGRLDHYEGIVQDITERRQMAEKLDRSRRKLRGLASEITRVE
                     ERERRAIAAQLHDQLGATLSMGKVRLGSLRQEAQGTPLAAGIEAVHALVGEAVRETRE
                     LTWELSPPVLYQLGLEAALEWLGESFEERHGIAFRFEREGERRDLGDERRFLVFAAVR
                     ELLLNVVKHADADGASVRLRWLETGVEVAVSDDGKGFEAAAPAPDESRSFGLFSIQER
                     FDDLGGSVAIRSRPGEGTTVALVLPYRAEAGEESE"
     gene            6391..6954
                     /locus_tag="P5164_05930"
     CDS             6391..6954
                     /locus_tag="P5164_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HRY43468.1"
                     /translation="MFEAFRARAEAVSAEVHRVGGSAEALAFVAEVLAKEGVAAQAGA
                     RAVWAPGPLVAGIDRDALAARIPGLTFDVTREAAAESRVGVSEMDWGLADTGTVVQDA
                     TDPALRLVSTLPEIHVALLPSDRILPSLGDVLPRLDPRTAPYVAFVTGPSRTADIERV
                     LTIGAHGPSRLVVVVHGAGAAPAEVTP"
     gene            6951..9149
                     /locus_tag="P5164_05935"
     CDS             6951..9149
                     /locus_tag="P5164_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HRY43469.1"
                     /translation="MTVTFRKEIAGALGDANLASALGRFYEAYPVSRAKAWQGIDFEA
                     VRTRIAEVKGHAAGRLAELAERFAKEAGARGAKVVLLKSPEEVRRYVVDLAREKGVKR
                     VAKSKSMASEEVHLNEALAEAGIDVRETDLGEWIIQLAGQRPSHMVMPAIHLTKEDVA
                     EIFSKEVGERLSTDIPRLVKVARKELRSAFLEADMGISGANVAVAETGTLVLVTNEGN
                     ARLVTSLPRIHVAIVGLEKLVERLADVVPILTALPRSATGQLLTSYVSMITGAAPNTD
                     GTPKELHVVLMDHRRTEMAADPVFREALQCIRCASCLNVCPVYRHVGGHVFGDVYTGG
                     IGTILTAWTGAMERSKEIQGLCIQCGNCVGVCPGKIDIPELIVEVRRRQVQEKGQPLV
                     QKAIFKVVNNRRLFHSVLRSASLAQKPFEKDGFVRHLPLFLSGLADFRSLPAIAEAPF
                     RDAFRAIEQRVVRAEEPAAPGAEARRMRKAAFYAGCLIDFAYPGMGGDVVKVLNAGGV
                     EVTFPETQTCCGAPARYSGAYEVAAQNAIDNIEALLAEGAEWVVSACPTCTTALRHEF
                     ARSLRSTGRTEWLAKAEALAEKVIDFSSLVTKLVDEGSLHIDPKKVPPVTYHDSCHLK
                     RTLHVSEEPRALLRKAGHEVVEMKESDTCCGMGGSYSLKFPEISKPILQKKLANIRDT
                     QASAAVMDCPGCVMQIRGGFDKSKDPVEVQHIARLLARAVADPAGDGSEP"
     gene            complement(9162..11171)
                     /locus_tag="P5164_05940"
     CDS             complement(9162..11171)
                     /locus_tag="P5164_05940"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HRY43470.1"
                     /translation="MSRARRVLLLALGALLAGAAMRGLLAVVEAALEAKVRARIVSEA
                     AERGFRAELERVRLRLPFRARLTGLALAGHGVTLRLDRVDVRAGFAGHGLVGRLRRLE
                     SGSISAELPHGLSVRVAPSEWDVSARGGVAWFSGRSGEGAFALGRTAGGGADLRADRL
                     DLTRLVRLRRDGVLVGDLGVVTGTLALSRTAGEGLAVEANLLSRQVRLGLPPEEDASA
                     EASLGEPFDAVGSVSATLHPEARFVEVACATGDAAGLAGSARGVGATAPGDAWIDVAL
                     VIPRLELGPLLAASGLELPAEGGDLGSAALEGRISGRMLDPKSWVVEEALDFRPPAQP
                     LPEIERLRGPFRRVMTGRDGRRTPVAVDPESPDFVPLEEVPAHLVRGLLTSEDADFWG
                     HPGIDLGEIPVAASMNWVRGRRARGASTITQQLAKNLFLTRERSYRRKLQEAALALLL
                     ESSLGKRRILEIYLNVIEWGPGLHGLGPAARHYFGKNPQDLTPREGAFLVALIPGPIK
                     YQSSFAGGVLSRGFTRLVNGVLRRLASVGMLTDEELKVELETPLRLRWTPADVAGEEL
                     VAGGAGSGALEPDEEALDEVGVPPLDPVAPAAAGDEPAPSGLLPSTAPPPPAAGPAAG
                     AGAVPGSAAAPTPVATPLAKEPSGPPIPATPAVPRPVPRATAPAV"
     gene            11292..11921
                     /locus_tag="P5164_05945"
     CDS             11292..11921
                     /locus_tag="P5164_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY43471.1"
                     /translation="MDQEEKSERSRAQILEAALELFSRQGYRGTSIRDISKAASVSTG
                     SVYHHFRDKEELFDTLLSQYWAAIESPDFPFNRALASGAFPDRLEELAEAAEASVRAW
                     ERHVALIYVDVVEMQGTHIQRFYSGMAERFAAFLRRYHAQDDFEARLTPDVSPLSAVM
                     LASRIFLQYYAVERLFHVPDQFGKPSKEAIRDISEILRRGMLRERRPAR"
     gene            12076..>12272
                     /locus_tag="P5164_05950"
     CDS             12076..>12272
                     /locus_tag="P5164_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43472.1"
                     /translation="MAAAPPGREIVMSLVEERLEAVGRVPLAVLPTPLQEAPRLAAHL
                     GLAGLLVKRDDLTGLAMGGNKA"
BASE COUNT         1887 a         4369 c         4341 g         1675 t
ORIGIN      
        1 ttcgtctcgc gggcgaggcg gaagagcgcg gcgacctcgt ccacggactc cgtcagaacg
       61 acggccttcg ggatcaggcg gtagaagctc gcgtccgagg cgaacgcgat ccggtccagg
      121 ggtcgatcga gaacgcgctc gggcgcggcg atccgctcga gggcggtgcg aagccccgcc
      181 ggcgaggccg gcatgccgga cgcggggacc gtggagccat ccggggcggt cgtcatgcga
      241 ggccctcctg ggcggcgtgc gcggcgccgg ggggattctc ccccgccggc gccgctccgc
      301 ccgaggtcag aagtagaacg cctggagctg gaccgtgaac tggttcgtcg gcttcacggc
      361 cgagctcttc ggctcgaccc tcaggacctg ggccgtcagc ttgaagttct gcccggagac
      421 gtaccagttc agcccgccgc cgaagcgctc gatgtccttc ccctcgtcgg ccgcgttcgc
      481 gaacgactgg ctctcgtact tgaggaaagg ctggaccttc gccgaggaga ggtagtacgc
      541 cgcctcgacg aggaggtcgt cctgcttcgg gatcgacgcg aggaagtcgc cgccgtcgta
      601 ccggatccac tggacctggc ccccgacctc gtcccccttc gccaccggga tcgtcgcgaa
      661 gacgtcgccg ctgaaggcgc ggtagtcctt ctggaggtcg gcgccggcgc tcacgttgac
      721 gaccttcttc ttcccgaggt tcgtcccggc gaagacgtag ccgcgctccg tctcgaagaa
      781 gttgtactgg aggtagccgg ccgtccggaa cgagttgcgc gaggcggagt cgcgcacgcc
      841 ctgcatcacc gcggcgcggt actcgaggcg cccgccgtcg acgaggtacc ccttcgcctg
      901 gaagccgttg tcgcgcaggc cgttcgtctg cgtcggaccg gccatgaccg tcgaggcggg
      961 gctgatgtcg agcgtgaaga acgagagcgt cgactggagg atgttgcggc tgagcgggac
     1021 gaggaagtgc cccgcctcga gggtgagcgc gtcgttcagc ttccaggaga cgaaggcgtc
     1081 ctggatgacg aagccggtgt tcgtctgctt cgctccggcc gggttcacct tcccgaggtt
     1141 cccgctgtcg gtctggaaga agaacgtgac gtccttcgcg acctggcccc cgacgaggag
     1201 gcgggcccgc cgcgcgaaga ggttctgggc atagccgccc gtcgcggcgc tctgggtcca
     1261 gtcggcccag ccctggaact ggaagccgag cttgaagttc acgttgtcgt tgaccttgat
     1321 cacggcctgg gccgcggcgg gcgacgggag gaaggcgacg gcgagcgcgg cggcggccgc
     1381 ggcgcgggcg agcgtcagaa cgagcttcat cgggcgcctc acttcgcgac cgcttcgacg
     1441 gtcgggatca tccaggggaa gacgtaggcc tgaaggaaga cgaggacgcc gacgaggcag
     1501 gcgaggacga tcgaatgcca gaagacgtac cggaggatcg tcccttcctg gccctgctgg
     1561 ttcgtcgcga cgctcgccac gacgatgctc tgcgcgtcga tcatcttccc catgacgccg
     1621 ccgctgctgt tcgccgcggc cgcgaggacc gggttgatgc cgagcttctc ggcgctgatc
     1681 atctgcagcc cgccgaagag gacgttcgag gaggtgtcgc tcccggtgag cgccacgccg
     1741 agccacccga ggagcggcga gaagaaggga aacaggactc ccgtcgaggc gaaggcgagg
     1801 cccatcgtcg cgtcgaggcc cgcgtagcgc gtcgagaagc cgagcgcgag catggcgccg
     1861 atcgtgagga gcgagatcct cacccgcatc agcgtccccc agaagacctt tgcgatctcc
     1921 ttcagagaga agccgagcag gaggccgctc aggatgcccg agaggagaag gctcgtcccg
     1981 gtggcggaga gccagttgaa cgtgaagacc gcctcctcgg gcttctcctc cttcacgacc
     2041 ggcggcgcct tgatgaccgc cttgtggagg ccggggacct cccacttcag catcgagaca
     2101 cccttgagga cgttcgggga gtccttcttg ccgccgttca ggacgtcctt gacctgcggg
     2161 agaccccacg cgaagacgaa gacggagagg aggatccagg gcatccaggc catgagcgcc
     2221 cgcttcgggg tgtggaccgg gcgcgccgcg gcctcggcgg cccggtcggc gtggttctcg
     2281 tggtcgaacg tccaggtctt cttcggctgc cagaacttca ggaggaggac ggtcgccccc
     2341 atcgagacga tggcgcccgc gatgtcgacg acccaggggc cgtggtagtt cgagacgagg
     2401 aactgcgtga tcgcgaagct gaggcccgag gtgaggcagg cgggccagac ctcccacatc
     2461 gccttgcggc ccgacatcgc ccagacgagc cagaacggaa cgaggagaga gaagaacggc
     2521 agctgccgcc cggccatcgc ggagagcgcc tggacgtcga gcccggtgac ggaggcgagg
     2581 acgacgatcg gcgtcccgag cgcgccgtag gcgacgggcg ccgtgttgcc gatcagcgcg
     2641 atcccggccg cgaggagcgg ccggaagccg aggccgatga gcatggcggc ggagatcgcg
     2701 accggcgtgc cgaagcccgc ggccccctcg atgaacgccc cgaacgagaa cgcgatcagg
     2761 agcgcctgga tccggcggtc gtcggcgagc cccgcgacgg tgtgcttgac gacctcgaag
     2821 tcgcccgact tgaccgtcag gtcgtagacg aagatcgcgc agaggacgat ccagccgatc
     2881 gggaagagac cgtaggccgc cccgttcagc gccgacgtga gggcgagctt cgtcggcatc
     2941 ccgtaaacga agatcgcgac cccgagggag gtcgcgagcc ccaggagcgc cgccttgtgc
     3001 gccttcacgt ggaagaacgc gagcagcccg aggagcacca cgaccggcag cgccgccacg
     3061 agagccgaca gcaggaggtt ccctgccacc ggcgtgtaga tctgggtcca ggtcaacatc
     3121 agcccctcct catcccgacc agatgggcct aaggatggga gggcgaatac ttaggtgcaa
     3181 tgatgaagat tcatcaattt atgcgtgttt atacctagag aaagcaacca tgacggcagc
     3241 ggagtcctac gatccgccgg ggggcttggt cagggtgtcg cgagcccctc tcgaatggcg
     3301 aagcgcgtca gctcggcggc cgaatggatc tcgagcttgt tcatgaggtt ctgcttgtgg
     3361 acctcgaccg tcttgctgct gatctcgagc tcctgggcga tctcgcgggg gcgcttcccg
     3421 ccggcgagga gcctcaggac ctgccgctct ctcggggtga gcgacgcgag cgacggagag
     3481 ccgggcgtcg acgccttacg gacgagctcc tcgacgacga gcccggcgat cgaggggctg
     3541 agatacgtct tccctgcgag cgtcgtccgg atcgcgaggg cgagctcctc gaacgcgccg
     3601 tccttaagaa cgtagccgag cgcgcccgcc cggaggacct cggcgacgaa ccgccggtcc
     3661 gcgtacatcg acagcgccac gatccgcgtc ccggggagct ccgcaaggat gagccgcgtc
     3721 gcctcgacgc cgttcagctc gggcatcgcg atgtccatga cgacgacgcc gggccggaga
     3781 aggagcgcct gcgagaccgc ctcgcggccg tccgcggctt ccccgacgac ctccatctcc
     3841 ggctgctggg agaggagggt ccggaggccc tcacggacga tccggtggtc gtccgcgagc
     3901 aggatcgtcg tgctcattcg ctctcctccc ccgcctccgc ccgataaggt agcacgaggg
     3961 cgaccgtcgt cccctcgccc ggacgggaac ggatcgcgac gctgccgccg aggtcgtcga
     4021 accgctcctg gatgctgaag aggccgaagc tgcggctctc gtccggcgcc ggggcggcgg
     4081 cctcgaaacc cttcccgtcg tcgctcaccg cgacctcgac gccggtctcg agccagcgaa
     4141 gccgcacgga cgcaccgtcg gcgtccgcgt gcttgacgac gttcaggagg agctcgcgga
     4201 cggccgcgaa gacgaggaac cgccgctcgt cgccgaggtc cctcctctca ccctccctct
     4261 cgaagcggaa cgcgatgccg tgcctctcct cgaagctctc gccgagccac tccagggccg
     4321 cctccaggcc gagctggtag aggaccggcg ggctcagctc ccacgtcagc tcccgggtct
     4381 cccggaccgc ttcgccgacg agggcgtgga ccgcctcgat cccggcagcg agcggcgtcc
     4441 cctgcgcctc ctgccggagg gagccgagcc tcaccttccc catcgagagc gtggccccga
     4501 gctggtcgtg gagctgcgcg gcgatcgccc gcctctccct ctcctcgacc cgtgtgatct
     4561 ccgaggcgag cccgcgcagc ttccggcgcg aacggtcgag cttctccgcc atctgccgcc
     4621 gctccgtgat gtcctggacg atcccctcgt agtggtcgag cctccccttt tcgtcccgca
     4681 cggcccgagc gttctcgagg acccagatga gcgcgccatc cttcctcctc acgagcgact
     4741 cgaagccgcg gacctcctcc tcctcgacca gcgcctcctc gaagcgttcc cggctcgacg
     4801 ggtcgacgta gaagtcgcgc cggatgtccg agatcgactc cgtcatctcg gccggagagg
     4861 cgtagccggc catcgccgcg agggcggagt tgaccgtcag gaaccgcccg gaaggtgtcg
     4921 actggtagat cccttcggcc gcgttctcga agatgctcct gaggttcgac tccgcgaccc
     4981 ggagggcgcg ctcggtcctc gcgcgctcca ggatgtccct ccggagctcc tcgttcgccc
     5041 gcgagagctc ctccgaacgc tgccggatcg tctcctcgag cgcctcctgg tgccggcgga
     5101 gcgcctcctc ggcgagcttg cgccggctga tgttcagagc caggaccgcg ttcaggaccc
     5161 cgaggaggac gagtgccccg gcgccggcga ggaagacctt ccggtagcgc gcgaaagccg
     5221 actccgggag gttgaggacc gtgctcccct tcgggagccg cgaggcgggg atcccccagc
     5281 gcgcgagcgc gaggtggtcg aagaggtccc ggcccggcag gccgcggagg accgggatcg
     5341 attccggagc ctccccgcga aggatccgga gcgcgatccg tgcggcggcc tccccctggt
     5401 cgaacccgct cgtcagccgc ccccccacga tcccgaggcc gaggttgaag tcccaggctc
     5461 cgtagaccgg aacgctcgac gcgcccgtca cgagtcgcgc gctctcgtca tagccgaaga
     5521 cgcggccgtc ggcgtcgcgg aagaagaagg cgaagaagac gaggctgtcg ggggggaggg
     5581 tccggaggcg cgcgagcagc cgctccatcg agagaccgtc gagcggctcc atcgggacgc
     5641 ccctcaccgc gggtcccagc tcggcgagcc gggccctcac cgcgaggccg gtctccgtgt
     5701 cgtcgttgac gacgtagatg cgccgcgtgc cggggtggag gtcgagcgcg gtctcgaggc
     5761 tcgccccgag gtccgcgtcc tcgctcacgc cggtgaagag gcgccgcccc ttcagcttct
     5821 cttcgcggaa gtagttgacg ccgcagaaga cgacgggcgt cccggggaag acctcgtcgc
     5881 ggtgctcgac gaggaagtcc accgcgcggt cgtccacgcc gacgacgagg tcgatccggt
     5941 ggtccgcgta cttcctcctg tagacctccg ggagctgggc gacgtgccgc ggctcgtaga
     6001 aacgcttcgt gtccatgtac tccacgaaga cgttctcgac gccgaggacc ggctcgagga
     6061 ccgaggtgat tccgcgatgc tcgtcgtcgg cccacttgta gcccttgtag tacgagtgga
     6121 gaacgaggac gcggtggccg gacgccgcgt cggccccgga ggtggcggct cccgcggcga
     6181 cggcgccggc ggccggcccg agaaggagcg cgagcgcggc gaggagagcg cgaaacctca
     6241 ttccgacccc gggatggagg ctagcaggcc gctccggaag ctccagacga ccgaagctca
     6301 cgggtcgaga ataacctaga tatttttacg tagattcaga taagctgacc cgtggtcacc
     6361 ggagagcgct ccgggcccgg gaggcgagcg atgttcgagg catttcgcgc acgagccgaa
     6421 gccgtcagcg ccgaggtcca ccgcgtcggc gggagcgccg aggcgctcgc gttcgtcgcg
     6481 gaggtcctcg ccaaggaagg ggtcgccgcc caggccggcg cgcgggcggt ctgggcgccg
     6541 ggtccgctcg tcgcggggat cgaccgcgac gcgctcgccg cccgcatccc cggcctgacg
     6601 ttcgacgtga cgcgggaggc cgcggccgaa agccgcgtcg gcgtcagcga gatggattgg
     6661 gggctcgccg ataccggaac cgtcgtccag gacgcgaccg acccggcgct gcggctcgtc
     6721 tcgacgctcc cggagatcca cgtggcgctc ctcccttcgg accggatcct ccccagcctc
     6781 ggggacgtcc tcccgcgcct cgaccctcgg accgccccgt acgtcgcgtt cgtgaccggg
     6841 ccgagccgga cggccgacat cgaacgcgtc ctgacgatcg gtgcccacgg gccgtcgcgt
     6901 ctcgtcgtcg tcgtccacgg cgccggggcc gcaccggcgg aggtgacgcc gtgacggtga
     6961 cgttccgcaa ggagatcgcc ggcgcgctcg gcgacgcgaa cctcgcgtcc gcgctcggcc
     7021 gcttctacga ggcctatccc gtcagccgcg cgaaggcctg gcaggggatc gacttcgagg
     7081 ccgtccgcac gaggatcgcc gaggtgaagg ggcacgcggc gggccggctc gccgagctcg
     7141 ccgagcgctt cgcgaaggag gccggcgcgc gcggcgcgaa ggtcgtcctc ctgaagagcc
     7201 ccgaggaggt ccgccggtac gtcgtcgacc tcgcccgcga gaagggcgtg aagagagtcg
     7261 cgaagtcgaa gtcgatggcc tccgaggagg tccacctcaa cgaggccctg gccgaggccg
     7321 ggatcgacgt gagagagacc gacctcggcg agtggatcat ccagctcgcc ggccagcgcc
     7381 cctcgcacat ggtgatgccc gcgatccacc tgacgaagga ggacgtggcg gagatcttca
     7441 gcaaggaggt cggcgaacgg ctctcgaccg acatcccacg gctcgtgaag gtggcgcgca
     7501 aggagctccg gagcgccttc ctcgaggccg acatgggaat ctcgggggcg aacgtcgcgg
     7561 tcgccgagac cgggacgctc gtcctcgtga cgaacgaggg gaacgcgcgc ctcgtcacgt
     7621 cgctcccgcg gatccacgtc gccatcgtcg ggctcgagaa gctcgtcgag cggctcgccg
     7681 acgtcgtccc gatcctgacc gccctcccgc ggagcgcgac ggggcagctc ctgacgagct
     7741 acgtctcgat gatcacgggc gccgcgccga acaccgacgg gacgccgaag gagctgcacg
     7801 tcgtcctgat ggaccaccgc cggaccgaga tggcggcgga cccggtcttc agggaggcgc
     7861 tccagtgcat ccgctgcgcc tcctgcctca acgtctgccc ggtctaccgg cacgtcggcg
     7921 ggcacgtctt cggcgacgtc tacacgggcg gcatcgggac gatcctgacg gcctggacag
     7981 gcgcgatgga gcgctcgaag gagatccagg ggctctgcat ccagtgcggc aactgcgtcg
     8041 gcgtctgccc ggggaagatc gacatccccg agctcatcgt cgaggtccgc cgccggcagg
     8101 tccaggagaa ggggcagccc ctcgtccaga aggcgatctt caaggtcgtc aacaaccgcc
     8161 gcctcttcca ctcggtcctc cggtcggcct cgctcgccca gaagccgttc gagaaggacg
     8221 gcttcgtgag gcacctgccg ctcttcctct ccgggctcgc cgacttccgg agcctcccgg
     8281 cgatcgccga ggcgccgttc cgcgacgcgt tccgggccat cgagcagagg gtcgtccgcg
     8341 cggaggagcc cgcggccccc ggtgcggagg cgcgcaggat gcggaaggcc gccttctacg
     8401 ccggctgcct catcgacttc gcctaccccg ggatgggcgg ggacgtcgtg aaggtcctga
     8461 acgcgggcgg cgtcgaggtg accttccccg agacgcagac ctgctgcggg gccccggccc
     8521 gctacagcgg cgcctacgag gtggccgcgc agaacgcgat cgacaacatc gaggcgctcc
     8581 tcgccgaggg ggccgaatgg gtcgtctccg cctgccccac ctgcacgacg gcgctccgcc
     8641 acgagttcgc gcggtcgctc cggagcacgg ggcggaccga gtggctcgcg aaggccgagg
     8701 cgctcgcgga gaaggtgatc gatttctcct ccctcgtgac gaagctcgtc gacgagggct
     8761 cgcttcacat cgacccgaag aaggtccccc cggtgacata ccacgactcc tgccacctga
     8821 aacggaccct ccacgtctcc gaggagccgc gcgccctcct ccggaaggcc gggcacgagg
     8881 tcgtcgagat gaaggagtcg gacacctgct gcggcatggg cggctcctac tccctgaagt
     8941 tccccgagat ctcgaaaccg atcctccaga agaagctcgc gaacatccgc gacacgcagg
     9001 catccgccgc cgtcatggac tgccccggct gcgtcatgca gatccgcggc ggcttcgaca
     9061 agtcgaagga ccccgtcgag gtccagcaca tcgcgcggct cctcgctcgg gcggtcgccg
     9121 accccgcggg agacggctcc gagccctgag ggaaggccgc ttcagaccgc cggcgccgtc
     9181 gcgcgcggga ccgggcgcgg cacggctggc gtcgcgggga tcggcggtcc ggaaggctcc
     9241 ttcgcgagcg gcgttgccac cggcgtcggc gccgccgcgg accccggcac ggcccccgcg
     9301 cctgccgcgg ggccggccgc aggcggcggc ggagcggtcg acgggagaag gccggacggg
     9361 gccggctcgt cccccgcggc cgccggcgcg acgggatcga ggggcgggac gccgacctcg
     9421 tccagagcct cctcgtccgg ctcgagcgcg ccggatccgg ctccgcccgc cacgagctcc
     9481 tcgccggcca catcggccgg cgtccaccgc agccgcagcg gcgtctcgag ctccaccttc
     9541 agctcctcgt cggtcagcat cccgacggag gccaggcggc ggaggacgcc gttcacgagc
     9601 cgggtgaagc ccctcgacag gacccctccc gcgaacgagg actggtactt gatcggcccc
     9661 gggatcagcg ccacgaggaa cgccccctcg cggggcgtca ggtcctgggg gttcttgccg
     9721 aagtagtggc gcgccgcggg accgaggccg tggaggcccg gcccccactc gatcacgttc
     9781 aggtagatct cgaggatccg ccgcttcccg agcgacgact cgagcaggag cgcgagggcc
     9841 gcctcctgga gcttcctccg gtagctccgc tcgcgcgtca ggaagaggtt cttcgcgagc
     9901 tgctgcgtga tcgtcgaggc gccccgcgcg cgccggccgc ggacccagtt catcgacgcc
     9961 gcgaccggga tctcgccgag gtcgatgccc gggtgtcccc agaagtcggc gtcctcggag
    10021 gtcagaagcc cccggaccag gtgcgccggc acctcttcga gcgggacgaa gtcggggctc
    10081 tccgggtcga cggccaccgg cgtccggcgg ccgtcgcgcc ccgtcatgac gcgccggaac
    10141 gggccccgaa gccgctcgat ctcgggaagg ggctgcgccg gcgggcggaa gtcgagcgcc
    10201 tcctccacga cccaggactt cgggtcgagc atccgaccgc tgattcgccc ttcgagcgcc
    10261 gcgcttccga ggtcgccgcc ttcggcgggg agctcgagcc cggacgccgc gagcagcggc
    10321 ccgagctcga ggcgagggat cacgagggcc acgtcgatcc aggcgtcgcc gggcgcggtc
    10381 gcgccgactc ctcgcgccga gccggcgagg ccggccgcgt cgccggtcgc gcaggcgacc
    10441 tcgacgaagc gcgcctccgg atggagcgtc gcgctcacgg agcccaccgc gtcgaatggt
    10501 tcgcccaggg aggcttcggc cgaagcgtcc tcctcgggcg ggagcccgag ccggacctgc
    10561 cgggagagga ggttcgcttc gaccgcgagg ccttcgcctg ccgtccgcga cagggcgagc
    10621 gtcccggtga cgacgccgag gtcgccgacg agaacgccgt cccgccgaag ccggacgagg
    10681 cgcgtcaggt cgagccggtc ggcacggagg tcggcgccgc ctcccgccgt ccgcccgaga
    10741 gcgaatgccc cctcgccgga acggcccgag aaccaggcga cgccgccccg agccgacacg
    10801 tcccactccg acggagccac gcgaaccgag aggccatggg gcagctcggc cgagatcgaa
    10861 ccgctctcca gccgacgcag tcggccgacg aggccgtgcc ccgcgaagcc cgcacgcacg
    10921 tcgacccggt cgaggcgcag ggtgacaccg tggccggcca gcgcgaggcc cgtcagccgc
    10981 gcgcggaacg ggagccgaag gcgcacacgc tcgagctccg cccggaagcc gcgctccgcc
    11041 gcctcggaga cgatccgggc gcggaccttc gcctcgagcg ccgcctcgac gacggcgagg
    11101 agaccgcgca tcgccgcgcc cgcgagaagg gcgccgagcg cgaggaggag gacgcgacgc
    11161 gcgcgggaca tccggagccc cgactttacc gggaggcggc gtccgagcgc acgctcgact
    11221 tcggccggca gggacgccct tgacgccgga gcgcgcgccc cctacggttg ccgatcgagg
    11281 cggagtgcgg gatggaccag gaggaaaagt cggagcggag ccgggcgcag atcctcgagg
    11341 cggctctcga gctcttctcc cggcagggct accgcgggac gagcatccgc gacatctcga
    11401 aggcggccag cgtctccacc ggaagcgtct accaccactt ccgtgacaag gaggagctct
    11461 tcgacacgct cctctcccag tactgggcgg cgatcgagag ccccgatttt cccttcaaca
    11521 gggccctcgc ctcgggggcc ttcccagacc gtctcgagga gctcgccgag gcggcggagg
    11581 cgagcgtgcg ggcctgggag cgccacgtcg cgctcatcta cgtcgacgtc gtcgagatgc
    11641 aggggaccca catccagcgc ttctactccg gcatggcgga gcgcttcgcg gcgttcctcc
    11701 gccgctacca cgcgcaggac gacttcgaag cgcgcctcac gccggacgtg tcgccgctct
    11761 cggccgtaat gctcgcgagc cggatcttcc tccagtacta cgcggtcgag cgtctcttcc
    11821 acgtccccga ccagttcggc aagccgtcga aggaagcgat ccgggacatc tccgagatcc
    11881 tccgtcgcgg catgctccgc gagcgccgtc ccgcccgcta gggacgcggc tccgctccgg
    11941 cggcagcatc ccggcgcgaa ggaaggcggc gagcgccgcg gcgggtgcat cggtcgtcca
    12001 ctccagcgga ggtccggcgc cgccggcgcg ccggcgcccc tcgcccggcc ctcttcgccc
    12061 atactcgagg gtcgagtggc cgcggccccg cccggccggg agatcgtcat gagcctcgtc
    12121 gaggagcgtc tcgaagccgt cggccgcgtc ccgctcgcgg tccttccgac ccccttgcag
    12181 gaggcgccgc gactcgcggc gcacctcggc ctcgcaggcc tcctcgtcaa gcgcgacgac
    12241 ctgaccggcc tcgcgatggg ggggaacaag gc
//
LOCUS       DAOTEZ010000077        12159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3667_length_12159_cov_6.816094, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000077 DAOTEZ010000000
VERSION     DAOTEZ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12159
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3667_length_12159_cov_6.816094"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /locus_tag="P5164_05955"
     CDS             <1..1233
                     /locus_tag="P5164_05955"
                     /inference="COORDINATES: protein motif:HMM:NF017555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HRY43473.1"
                     /translation="VSYDLLPRESDILGRRFWQKAFDLLKDAGATQLETEGKNAGCWV
                     MKLEGAKEFEGMEDADKVLVRSNGTVTYTGKDVAYQLWKLGELGIDFEYEKAPYVNPF
                     GEAEFVASDGVTPLYRTRRDAEGADPSARGRFGGGDSVINVIDVRQSYPQKVVKEAVR
                     RAGFPEGADRSVHFAYEMVALTPASAELLGVTLSDEDRGRAYVEMSGRKGLGVKADDL
                     VERLVEKALEQILDRQPGERPDLSRAEAIAIGALRVYMTRYSRNKVIAFDFDEALAFE
                     GDTGPYLQYAAVRTANIFRKLEEKGLPGLLDAEEAASVASLDAAHLDDGLWDVVRTCG
                     RTQETFEKAAETFEVSLLVRHALAVGAAFHHLYHTHPILQAENDESRRARRAALQLVA
                     RTLDDVLGVLGVPVPERM"
     gene            1233..1781
                     /locus_tag="P5164_05960"
     CDS             1233..1781
                     /locus_tag="P5164_05960"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRY43474.1"
                     /translation="MLAGAAVRLRPVKADDLDWLVAACGKPSAFGEFEPFFVGEAESL
                     KRRFEQDGLLSEDLTRFVVEDRSGRRVGLAGVDDVDLHSRVARITATILEPAERGKGL
                     GTDAHRVLVSYLFLHRGLLRIEAFVAAGNVAARGVLKRLGFVEEGTLRSRVFAHGQRH
                     DVVVLGLLYEDWSRRSVASLAI"
     gene            complement(1736..2215)
                     /locus_tag="P5164_05965"
     CDS             complement(1736..2215)
                     /locus_tag="P5164_05965"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRY43475.1"
                     /translation="MTPRRVVAFRAAGVACAADPSDVERILRPASLLPAPGAPSWIAG
                     LASGPRGLLAVVDVGRRLFPEREAPPPRDLLAGTFDGIPAALGAEVVEGILTVEEAEG
                     LPPEGLAPVVAECLKGVVVAGGARRLLLDVRRLVTGEPARLAAQIASDATERRDQSS"
     gene            complement(2212..3939)
                     /locus_tag="P5164_05970"
     CDS             complement(2212..3939)
                     /locus_tag="P5164_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRY43476.1"
                     /translation="MAHEEPDRFIPILSLAAGLSAFTAGGAASVAAAWAAAGFGNPLR
                     QSLVLDLGLAASAVSGLLAGAAVAVFLQRPAARFGRILAAARQMAEGDLASRAPEGTD
                     LAGRIGRFLNAVAGRGAHLLSSVRREHGQLNRQVAVLRAASIRNRERAAQERVRLSSA
                     TAAVGGLDGTIRAIAESVETLAAGSEETAAAVAEVDGSLTQLLARSEGLLVDSTDGAG
                     AAASLAEGAAVLDATLSDLARRADNLAEGSAQAERAHGAVLASAREAAEQAAHAATEA
                     RSGRAVVDEARASVAAIRLSSGTVREAVGRVEARSREVGRILEMLEDIARQTNLLALN
                     ASFLATRAGEHGRGFSVVAAEIRRLSERTSEGARGIGGQVSGMREEVDAARAAAEEET
                     RLVTRGVETATRAGEALAGIEAAAARAEEAVAAIREVSRAQAATVAASVVSVGEMREG
                     LRALAEEGRRNTREAERIRELVGRIRELAGFVERTVQEQKGAAGQIAVAAERSLGLMR
                     DIQDAVGRQTADSHRILGLLSEVEAMSLDTLASAASVEDTTAALETLAGSLEDEVGRF
                     RVDAELQAT"
     gene            complement(3963..4646)
                     /locus_tag="P5164_05975"
     CDS             complement(3963..4646)
                     /locus_tag="P5164_05975"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HRY43477.1"
                     /translation="MDCETATPLIEALCDDELDVATAARLLAHLDACEPCSALRRDAE
                     TRNAALREARPDDQIPDAIRTRLARAVAKAPRARRTRRRGVLAGAAVLAAAGAIGAAL
                     LVRGAPPPVPTLVEASEVRELTGEVFCLRCALARLFPETPVLDPRHLPVLRTDDGEVV
                     TLLDGAVTESVLARKGCAGRRVALTLRLFPRQELAEVLEARELGPASGPLPGEVVATA
                     VRQSSSSPP"
     gene            complement(4681..5343)
                     /locus_tag="P5164_05980"
     CDS             complement(4681..5343)
                     /locus_tag="P5164_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRY43478.1"
                     /translation="MALFGFRRDRAPNAWDLRSGELPYADALFGTALRLTRNRQDAED
                     LLQETYLRAFAHYDGFREGTNLKAWLFRILKNGFINRYRQLKAGPREVDLERGGATFE
                     SALEDAVAPAATPEDELLGRSLDGDVARALASLPEDFRLVVELVDLQDFSYREVADIL
                     EIPLGTVMSRLYRGRRLLEETLLAYGKRRGYFGPGTSPARSRRAAGEGTGPAEYPVRR
                     RR"
     gene            complement(5453..5782)
                     /locus_tag="P5164_05985"
     CDS             complement(5453..5782)
                     /locus_tag="P5164_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRY43479.1"
                     /translation="MIKKLLPAVAVAGLAFLLPNLAFAQEAAEAAKATSGGAGWGHLS
                     AALVLAIGATAGATAQGRTAVAACDGMARNPGAAGQIRGIAFLGLVLIESLVIYTLVI
                     AFLLQGK"
     gene            complement(5820..6617)
                     /gene="atpB"
                     /locus_tag="P5164_05990"
     CDS             complement(5820..6617)
                     /gene="atpB"
                     /locus_tag="P5164_05990"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRY43480.1"
                     /translation="MQHHHSILFGPVNALLTAIFGSPENAPSWWLHGVTIGGVHVAGV
                     APVDHATGAVLGWVPDHVIMALVVLLVFAVVLPILARRFRDGVPGGAQNVLEVFVDFL
                     RGLIKENIPHTGEKYLPVVGAFFFFITLSNLVGQLFFLQPPTSSLNTTFALSLSCLVF
                     FNAAGIKAHGFFGYLKTFAGPSAALAILVFPIEMIGNFARALSLAMRLFGNIYGEHTA
                     TNQFVELVPLLVPMPMNALGIFGSFLQAYVFTLLTTVYIGGAIAHEH"
     gene            complement(6619..7044)
                     /locus_tag="P5164_05995"
     CDS             complement(6619..7044)
                     /locus_tag="P5164_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43481.1"
                     /translation="MSLALLPQERRVFRVASLAALLAALALGALGKFPEAAALTAGAA
                     VAIVSGLWLSDVAGRLLVPRPRASTRLDWKFALRALLRYAFLGLAAYGSVRLFPDEVP
                     WLLGGLSAVVLGVLADGFRGGAAGRSGPEEEEEGTNPRE"
     gene            complement(7041..7316)
                     /locus_tag="P5164_06000"
     CDS             complement(7041..7316)
                     /locus_tag="P5164_06000"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRY43482.1"
                     /translation="MPEPRREPDPNLRLLGDVLSFGWVLPACLAAGGGLGWLLDRWLG
                     SFPAATAVLGLLGFAGGLRQLLREAERLEGRRKGPPGSGPGPAGDGA"
     gene            complement(7324..8802)
                     /locus_tag="P5164_06005"
     CDS             complement(7324..8802)
                     /locus_tag="P5164_06005"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HRY43483.1"
                     /translation="MALPFPLSDLLVILPELILSELALGILLWAAVVKRERERALAWA
                     SLASLAIAAALLPVAASFAGELPRSAFGGMIALDGFSLFFKALTLLAAAVTILFSLRF
                     VGTSPYPGGEYYALLLLTTVGMMFMASGTHLVSIYVALELMALSQYVLAGYFKQETKS
                     VEAAAKYFVLGAFSSGVLLYGLSLVYGATGTLSLGGVSAALEAAPRSTLLMVGVVLVA
                     CGMLFKVASAPFHVWAPDVYEGAPTPVSAFFAVGPKLAAYAIFARIFFDGFGPRSDDW
                     APVLAASAALTMVVGNVGALMQTNVKRLLGYSSIGHAGYALLGLLAYRAGGAAGQPEA
                     FGLWAILFYLTAYLLMTFGAFGLVVLLEAKGYATESVDDFNGLWKRNPLAAGAMVVFL
                     LSLAGIPPTAGFVGKYLLFSAALKAGWPALVVLGVLMSAVSLFYYFRIVRAMYLVDGE
                     GEVRWREEPAVAVAVGLCFAGTLAFGVLPQPLIALAKSCLLP"
     gene            complement(8806..10443)
                     /locus_tag="P5164_06010"
     CDS             complement(8806..10443)
                     /locus_tag="P5164_06010"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRY43484.1"
                     /translation="MNGLLSLLILLPTAGALAILFLPREKTGPIKALATAVAGLTFFA
                     SLPLWFARGFRESAGFLFREDLSWIPSLGVRYSLGVDGIAALLILLTTLLSVISIYAS
                     YTAVKTREKEYYIFLLLLETGMIGVFCALDFFLFYVFWEVMLVPMYFLIGVWGGPRKL
                     YAAIKFFLYTLFGSVIMLVGIIALYFFNTTGFLWWKGLGNAPTFDILHFHAIAPQIPA
                     DLQLWLFWAFFLGFAIKVPMFPFHTWLPDAHVEAPTAGSVILAGVLLKMGTYGFLRFS
                     LPIFPSAASTRWVQLTVVVLAIVGILYGSLVAMVQKDIKKLVAYSSVAHLGFVMLGFF
                     ALNNAGMQGGVLQSINHGLSTGGLFLLVGIIYERRHTRQIADFGGLSQVMPIYATLFM
                     IIALASLGLPLLNGFVGEFTILVGAFLADWKWALFAGVGVILGAAYLLWLYQRVFFGE
                     LKHEENKGLQDVNLREVLTLAPLIVCCVWIGVYPKPFFAMTAPASAKIVAALDAAKGA
                     PPALSAALSAAPAAPVPSATAVLPAAAALPEAAPRGN"
     gene            complement(10460..>12159)
                     /locus_tag="P5164_06015"
     CDS             complement(10460..>12159)
                     /locus_tag="P5164_06015"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRY43485.1"
                     /translation="FAMLTLVLGANLAILFVGWEGVGLCSYLLIGYYYTKDFAADAGK
                     KAFIMNRVGDWGFLMGLFACVAMFGTVDFGKMFAAAAATPATYAAGGLVTFACLALFV
                     GATGKSAQIPLYVWLPDAMAGPTPVSALIHAATMVTAGVYMVVRCNVLFRISHEWALK
                     TLGEGASVWGLLTHDASFVVAVVGGVTAFVAATIGLAQTDIKKVLAYSTVSQLGYMFL
                     GCGVLAFGAGMFHVFTHAFFKACLFLGSGSVIHALSGEQEMTAMGGLRSKIPHTYRTM
                     LIATLAISGVPLLSGFFSKDLILGETFLQGHPWLWALAALTAGLTAFYMFRLIKMTFF
                     GEYRGPKETWEHVHESPATMTVVLWILAVGAVVVGYVGIPNFIVPGGNRFEAFLHPAF
                     PALPPDWTPHPHQASHALEYGLMLGSVAIAGLGILLATTFFYKGSAPWALPAKVVAAF
                     PNAYRWVRNKYFVDEAYEAVFVNGLVKKGGRLLWEIDARVVDGVVNGTRHLTVGLSHV
                     SNWFDRTFVDGAVNGVGDSFQAAWKGYGKLQSGQTQNYAFGMAAGVFALVCIYIIFA"
BASE COUNT         1987 a         4205 c         4498 g         1469 t
ORIGIN      
        1 gtctcctacg acctccttcc gcgcgagagc gacatcctcg gccgccgctt ctggcagaag
       61 gcgttcgacc tgctgaagga cgcgggcgcc acgcagctcg agacggaggg gaagaacgcg
      121 ggctgctggg tcatgaagct cgagggggcg aaggagttcg aagggatgga ggacgccgac
      181 aaggtcctcg tccgctcgaa cggcaccgtc acctacacgg gcaaggacgt cgcctaccag
      241 ctctggaagc tcggcgagct cggcatcgac ttcgagtacg agaaggcccc gtacgtgaac
      301 ccgttcggcg aggcggagtt cgtcgcctcg gacggggtga cacccctgta ccggacgcgc
      361 cgcgacgcgg agggggcgga cccttcggcg cgcgggcgct tcggcggcgg cgactccgtc
      421 atcaacgtca tcgacgtgcg gcagtcctat cctcagaagg tcgtgaagga ggcggtccga
      481 cgggccggct tccccgaagg ggccgaccgg tcggtccatt tcgcgtacga gatggtcgcg
      541 ctgacgccgg cgtcggccga gctcctcggc gtgaccctct cggacgagga ccgcggccgc
      601 gcgtacgtcg agatgtcggg ccggaagggg ctcggcgtga aggccgacga cctcgtcgag
      661 cggctcgtcg agaaggcgct cgagcagatc ctcgaccggc agccgggcga gcgtcccgac
      721 ctctcccgtg ccgaggcgat cgcgatcggc gcgctccgcg tctacatgac gcggtactcg
      781 aggaacaagg tcatcgcgtt cgacttcgac gaggcgctcg cgttcgaagg ggacacgggg
      841 ccgtacctgc agtacgccgc cgtccggacc gcgaacatct tccgcaagct ggaggaaaag
      901 gggctccccg gcctcctcga cgcggaagag gcggcctccg tcgcatcgct cgacgccgcg
      961 cacctggacg acggcctctg ggacgtcgtg aggacctgcg ggcggacgca ggagacgttc
     1021 gagaaggccg ctgagacgtt cgaggtgtcg ctcctcgtgc gccacgccct cgccgtcggc
     1081 gccgccttcc accacctcta ccacacgcac ccgatcctgc aggccgagaa cgacgagagc
     1141 cgccgggccc gccgggccgc gctccagctc gtcgcgcgga cgctcgacga cgtcctcggc
     1201 gtcctcggcg tgccggttcc ggagaggatg tagtgctcgc cggggccgcc gtccgcctcc
     1261 ggccggtgaa ggcggacgac ctcgactggc tcgtcgccgc ctgcgggaaa ccctccgcct
     1321 tcggcgagtt cgagccgttc ttcgtcgggg aggcggagag cctgaagcgg cgcttcgagc
     1381 aggacggcct cctgtccgag gacctgacgc gcttcgtcgt cgaggaccgc tccggccgcc
     1441 gcgtcgggct cgccggggtc gacgacgtcg acctccactc ccgcgtcgcc cggatcaccg
     1501 ccacgatcct cgagccggcc gagcgcggga aggggctcgg gacggacgcg caccgcgtcc
     1561 tcgtctccta cctgttcctc catcgcgggc tgctccggat cgaggccttc gtcgccgccg
     1621 ggaacgtcgc cgcgcgcggc gtcctcaagc gcctcggatt cgtggaagag ggcacgctcc
     1681 gcagccgcgt cttcgcccac ggccagaggc acgacgtcgt cgtcctcggc ctcctctacg
     1741 aagactggtc gcgccgctcg gtcgcgtcgc tcgcgatctg agccgcgagg cgggccggct
     1801 ctcccgtcac gagccggcgg acgtcgagga ggaggcggcg tgcgccgccc gccacgacga
     1861 cccccttcag gcactccgcg acgaccggcg cgaggccctc gggaggaagc ccctcggcct
     1921 cctccaccgt caggatcccc tccacgacct cggccccgag cgccgcggga atcccgtcga
     1981 acgtcccggc gaggaggtcg cgcggcggag gagcctcccg ctccgggaag agtcgccggc
     2041 cgacgtcgac gacggcgagg aggccccggg gccccgaagc gagccccgcg atccaggacg
     2101 gcgctcccgg cgccgggagg agcgacgccg ggcggaggat ccgctcgacg tccgacggat
     2161 cggcggcgca cgcgacgccg gccgcccgga acgcgacgac gcggcgaggc gtcacgtggc
     2221 ctgcagctcc gcgtcgaccc ggaaccgtcc gacctcgtcc tcgagcgagc cggcgagcgt
     2281 ctcgagggcg gccgtcgtgt cctcgacgga ggcggcggac gcgagagtgt cgaggctcat
     2341 cgcctcgacc tcggagagga gcccgaggat ccggtggctg tcggccgtct gccggccgac
     2401 ggcgtcctgg atgtcccgca tcaggccgag cgacctctcc gccgcgaccg cgatctgccc
     2461 cgcggccccc ttctgctcct ggaccgtccg ctcgacgaag ccggcgagct cgcggatccg
     2521 cccgacgagc tcgcggatcc gctccgcctc gcgggtgttc cgccgcccct cctcggcgag
     2581 cgcccggagc ccctcgcgca tctcgccgac cgatacgacg ctcgcggcca cggtggcggc
     2641 ctgggcgcgc gacacctcgc ggatcgcggc gacggcctct tccgcgcggg ccgccgcggc
     2701 ctcgattccg gcgagcgcct cgccggcgcg agtcgccgtc tcgacgccgc gggtgacgag
     2761 ccgcgtctcc tcctcggccg ccgcccgcgc cgcgtcgacc tcctccctca tcccggagac
     2821 ctggccgccg atcccgcggg ccccctcgga ggtccgctcg gagaggcgtc ggatctccgc
     2881 ggcgacgacg gagaagcctc gaccatgctc ccccgcgcgc gtcgcgagga aggaggcgtt
     2941 cagggcgagg aggttcgtct gccgggcgat gtcctcgagc atctcgagga tcctgcccac
     3001 ctcgcgggag cgagcctcga cacgcccgac cgcctcgcgg acggtcccgg acgacaggcg
     3061 gatcgccgcg acggaagcgc gcgcctcgtc gacgacggcc cgccccgaac gggcttccgt
     3121 ggcggcgtgc gcggcctgct ccgcggcctc gcgcgccgac gcgaggacgg cgccgtgcgc
     3181 cctctcggcc tgcgccgagc cctccgcgag gttgtcggcc cgccgcgcga ggtcgctgag
     3241 cgtcgcgtcg aggaccgcgg cgccctccgc gagggaggcg gccgccccgg ccccgtccgt
     3301 cgagtcgacg aggagcccct ccgaccgcgc gaggagctgc gtgagcgacc cgtccacctc
     3361 cgcgacggcg gcggccgtct cctcggagcc cgccgcgaga gtctcgacgc tctcggcgat
     3421 cgcgcggatc gtcccgtcca gcccgccgac ggcggcggtc gcgctcgaga gccgcacgcg
     3481 ttcctgggcg gccctctcgc ggttgcggat tgacgcggcg cgcaggaccg cgacctgccg
     3541 gttgagctgc ccgtgctccc gccggaccga cgagaggagg tgcgcgccac gcccggcgac
     3601 cgcgttcagg aaacggccga tccgtcccgc caggtccgtc ccttcgggag ctcgcgaggc
     3661 gaggtcccct tccgccatct ggcgggccgc ggcgaggatc cggccgaaac gcgcggcggg
     3721 acgctgcagg aagaccgcga ccgccgcccc cgcgagcagg cccgagaccg ccgaggcggc
     3781 gaggccgagg tcgaggacga ggctctgccg gagcgggttt ccgaatccgg cggccgccca
     3841 cgccgccgcg acggacgccg cgccgcccgc cgtgaaggcc gacagccccg cggcgaggga
     3901 gaggatcgga atgaagcggt cgggctcctc gtgcgccacg ggggcagtct agaccagccc
     3961 gctcagggcg gggaggacga ggactggcga accgccgtcg cgacgacctc tcccggcaac
     4021 ggacccgaag cgggccccag ctcgcgcgcc tcgaggactt cggcgagctc ctgccgcggg
     4081 aagaggcgga gggtcagcgc cacccgccgt cccgcgcacc cctttcgggc gaggaccgat
     4141 tccgtcaccg cgccgtcgag gagggtgacg acctccccgt cgtccgtcct gaggaccggg
     4201 agatggcgcg ggtcgagcac cggcgtctcc gggaagagtc gggccagggc gcaacgcagg
     4261 cagaagacct ccccggtcag ctcgcggacc tcggaggcct cgacgagcgt cggcaccggg
     4321 ggcggcgcgc ctcgcacgag gagagccgcc ccgatcgcgc cggccgcggc gaggaccgcc
     4381 gcgcccgcga ggacgcccct gcgccgggtc cggcgcgccc gcggagcttt cgcgacggcg
     4441 cgggcgaggc gcgtccggat cgcgtcgggg atctggtcgt ccggacgtgc ctcgcggagg
     4501 gcggcgttcc gggtctcggc gtcgcgacgg agcgcggagc agggttcgca ggcgtcgaga
     4561 tgcgcgagaa ggcgggctgc cgtcgcgacg tcgagctcgt cgtcgcagag cgcctcgatg
     4621 agcggggtgg cggtctcgca gtccatcgcg cggcccgcgt gggggcctct ccagcgaggt
     4681 ctaacgccgg cgtcgcaccg ggtattccgc cggccccgtc ccctcgccgg ccgcacgccg
     4741 ggagcgcgcc ggcgaggttc ccgggccgaa gtacccccgg cgcttcccgt aggccaggag
     4801 cgtctcctcg agcaggcggc gcccccggta gagccgcgac atgacggtac ccagggggat
     4861 ctcgaggatg tccgcgacct cgcggtagga gaagtcctgg aggtcaacga gctcgacgac
     4921 gagccggaag tcttcgggga ggctcgccag cgcgcgggcg acgtccccgt cgagcgaacg
     4981 gccgaggagc tcgtcctccg gcgtcgccgc cggcgcgacc gcgtcctcga gggccgactc
     5041 gaaggtcgcc cccccgcgct cgaggtcgac ctcccggggc cccgccttca gctgacggta
     5101 gcggttgatg aacccgttct tcaggatccg gaagagccac gccttgaggt tcgtcccttc
     5161 ccggaagccg tcgtagtggg cgaaggcccg caggtaggtc tcctggagga ggtcctccgc
     5221 gtcctggcgg ttcctcgtca ggcgcagggc cgtcccgaag agggcatccg cgtacgggag
     5281 ctcccccgat cggaggtccc aggcgttcgg ggcccggtcg cggcggaatc cgaagagggc
     5341 catcttcctc ccttaacgac ggctcgcgcc cgccggattc cggccgatac ggcaaggccc
     5401 cggcaggcgc cggggccctc cgcaccgatc cgggccgcgc gggcccgccg ggtcacttcc
     5461 cctggaggag gaaggcgatg acgagcgtgt agatgacgag cgactcgatc aggacgaggc
     5521 ccaggaacgc gatgccgcgg atctggccgg cggctccggg gttgcgggcc atgccgtcgc
     5581 aggccgcgac ggccgtccgg ccctgggcgg tggcgccggc ggtcgcgccg atcgccagga
     5641 cgagcgcggc cgacaggtgg ccccagcccg cgcccccgct cgtggccttg gcggcctccg
     5701 cggcctcctg cgcgaaggcg aggttcggga gcaggaaggc gaggccggcg acggcgacgg
     5761 cggggagaag cttcttgatc atttcctttg ggctcctttc gggttcgatg atccgcgttt
     5821 caatgctcgt gggcgatcgc gcccccgatg tagacggtgg tcaggagggt gaagacgtac
     5881 gcctggagga acgacccgaa gattccgagg gcgttcatcg gcatcggaac gaggagcggc
     5941 acgagctcga cgaactggtt cgtcgcggtg tgctccccgt agatgttccc gaagagacgc
     6001 atcgccaggg agagcgcccg ggcgaagttg ccgatcatct cgatcgggaa gacgaggatc
     6061 gcgagcgccg cgctcggccc tgcgaacgtc ttcaggtagc cgaagaagcc gtgcgccttg
     6121 atcccggccg cgttgaagaa gacgaggcac gagagcgaca gggcgaacgt cgtgttcagg
     6181 ctggaggtcg gcggctggag gaagaagagc tggccgacca ggttcgagag ggtgatgaag
     6241 aagaagaacg cgccgaccac ggggaggtac ttctcgccgg tgtgcgggat gttctccttg
     6301 atgaggccgc gcaggaagtc gacgaagacc tcgaggacgt tctgcgcccc gcccggtacg
     6361 ccgtcccgga agcggcgggc gaggatcggc aggacgaccg cgaagacgag gaggacgacg
     6421 agcgccatga tcacgtggtc ggggacccag ccgaggacgg ctccggtcgc gtgatcgacc
     6481 ggggcgacgc ccgcgacgtg gacgccgccg atcgtcacac cgtggagcca ccacgacggc
     6541 gcgttctcgg gcgatccgaa gatcgccgtc aggagcgcgt tgacggggcc gaacaggatc
     6601 gagtgatggt gctgcatctc actcccgcgg gttcgttcct tcttcctctt cctccggccc
     6661 ggaccggccg gccgcgcctc cccggaaccc gtcggcgagg accccgagga cgaccgcgga
     6721 gagccctccg aggagccacg ggacctcgtc cgggaagagg cgaaccgagc cgtacgccgc
     6781 gagccccagg aaggcgtagc ggaggagggc gcggagcgcg aacttccagt cgagccgggt
     6841 cgacgcccgt ggacggggaa cgagcagtct cccggccacg tccgacaacc agaggccgga
     6901 gacgatagcc acggccgctc cggccgtcaa ggcggcggcc tccgggaact tcccgagggc
     6961 tcccagggcg agcgcggcga ggagagccgc cagggacgcg acccggaaga ctcgccgctc
     7021 ctgggggaga agcgcgaggc tcacgccccg tccccggccg ggccgggccc ggaccccgga
     7081 gggcccttcc ggcgcccctc gagccgctcc gcctcgcgga ggagctgccg gaggccgccc
     7141 gcgaagccga ggaggccgag gacggccgtg gccgccggga agctcccgag ccaccggtcg
     7201 aggagccagc cgagcccccc gcccgccgcg aggcaggcgg ggaggaccca gccgaagctc
     7261 aggacgtcgc cgaggaggcg gaggttcggg tcgggctccc tccgcggctc gggcatcggg
     7321 gcgctacggc aggaggcagg acttcgccag cgcgatgagc ggctggggga ggactccgaa
     7381 cgcgagcgtc cccgcgaagc agaggccgac cgcgacggcc accgcgggct cctcgcgcca
     7441 gcggacctct ccctcgccgt cgacgaggta catcgcccgg acgatccgga aatagtagaa
     7501 gagcgagacg gcggacatca ggacgccgag gacgacgagg gcgggccagc ccgccttcag
     7561 cgccgcggag aagaggaggt acttcccgac gaagccggcc gtcggcggga tgccggcgag
     7621 cgagaggagg aagacgacca tcgcgcccgc ggcgagcggg ttccgcttcc agaggccgtt
     7681 gaagtcgtcg acgctctccg tcgcgtagcc cttcgcctcg aggaggacga cgagtccgaa
     7741 cgccccgaac gtcatcagga ggtaggcggt caggtagaac aggatcgccc agagaccgaa
     7801 agcctcgggc tgccccgccg cgccccccgc ccggtaggcg aggaggccga gaagcgcgta
     7861 gcccgcgtgc ccgatcgagg agtagccgag gagcctcttg acgttcgtct gcatcagcgc
     7921 gccgacgttc ccgacgacca tcgtcagcgc cgcggaggcc gcgaggaccg gcgcccagtc
     7981 gtcggaacgc ggtccgaacc cgtcgaagaa gatgcgggcg aagatggcgt aggcggcgag
     8041 cttcgggccg acggcgaaga aggccgagac cggcgtcggc gcgccttcgt acacgtcggg
     8101 cgcccagacg tggaacggag ccgaggcgac cttgaagagc atcccgcacg cgacgaggac
     8161 gacgccgacc atcaggagcg tcgagcgggg cgcggcctcg agggccgcgg agacgccgcc
     8221 gagggagagc gtcccggtcg cgccgtagac gagggagagg ccgtagagga ggacgccgga
     8281 ggagaacgcc ccgaggacga agtacttcgc cgcggcctcg accgacttcg tctcctgctt
     8341 gaagtagccg gcgaggacgt actgcgagag cgccatgagc tcgagagcga cgtagatcga
     8401 gacgaggtgc gtccccgacg ccatgaacat catgccgacg gtcgtcagga ggaggagcgc
     8461 gtagtactcg cccccgggat agggcgaggt cccgacgaag cggagcgaga agaggatcgt
     8521 gaccgcggcg gccagaagcg tcagcgcctt gaagaagagc gagaagccgt cgagcgcgat
     8581 catcccgccg aacgccgacc gcggcagctc ccccgcgaac gacgccgcga cgggcaggag
     8641 ggccgctgcg atcgcgagcg acgcgagcga cgcccaggcg agcgccctct cccgctccct
     8701 cttgacgacg gccgcccaga gcaggatccc gagcgccagc tcggagagga tcagctcggg
     8761 caggatgacc aggaggtcgg agagcgggaa cgggagggcc acgggtcagt tccccctggg
     8821 agcggcctcg ggaagggcgg cggcggccgg gaggacggcg gtggcggacg ggaccggcgc
     8881 cgccggcgcc gcggagagcg cggcagagag ggccggcggc gcccccttcg ccgcgtcgag
     8941 agccgcgacg atcttcgccg aggcgggggc ggtcatcgcg aagaacggct tcggatagac
     9001 gccgatccag acgcagcaga cgatcagcgg cgccagcgtc aggacctcgc ggaggttcac
     9061 gtcctggagc cccttgttct cctcgtgctt cagctccccg aagaagaccc gctggtagag
     9121 ccagaggaga tacgccgcgc cgaggatgac gccgacgccg gcgaacaggg cccacttcca
     9181 gtcggcgagg aacgcgccga cgaggatcgt gaactcgccg acgaagccgt tcaggagggg
     9241 gagcccgagc gaggcgaggg cgatgatcat gaagagcgtc gcgtagatcg gcatcacctg
     9301 cgagaggccg ccgaagtcgg cgatctgacg cgtgtggcgg cgctcgtaga tgatcccgac
     9361 gaggaggaag agcccgcccg tcgaaaggcc gtggttgatc gactggagga cgcccccctg
     9421 catccccgcg ttgttcagcg cgaagaagcc gagcatcacg aatccgaggt gggcgaccga
     9481 ggagtaggcg accagcttct tgatgtcctt ctggaccatc gccacgaggg agccgtagag
     9541 gattccgacg atcgcgagga cgacgaccgt cagctgcacc cagcgcgtgg atgcggccga
     9601 cgggaagatc gggagcgaga accggaggaa cccgtaggtc cccatcttga ggaggacgcc
     9661 ggcgaggatc accgagcccg ccgtgggggc ctcgacgtgc gcgtcgggga gccacgtgtg
     9721 gaacgggaac atcggcacct tgatcgcgaa cccgaggaag aacgcccaga agagccagag
     9781 ctggaggtcc gccgggatct gcggcgcgat cgcgtggaag tggaggatgt cgaacgtcgg
     9841 agcgttcccg agccccttcc accagaggaa cccggtcgtg ttgaagaagt agagggcgat
     9901 gattccgacg agcatgatca ccgacccgaa gagcgtgtag aggaagaact tgatcgccgc
     9961 gtagagcttc ctcgggccgc cccagacccc gatgaggaag tacatcggga cgagcatgac
    10021 ctcccagaag acgtagaaga ggaagaagtc gagggcgcag aagaccccga tcatccccgt
    10081 ctccaggagg agcaggaaga tgtagtactc cttctcccgg gtcttcaccg ccgtgtacga
    10141 ggcgtagatc gagatgacgc tgaggagcgt cgtcaggagg atcaggaggg ccgcgatccc
    10201 gtcgacgccg agggagtacc tcacgccgag cgaggggatc caggagagat cctcgcggaa
    10261 gaggaagccc gccgattccc ggaagccgcg cgcgaaccag agcgggagcg aggcgaagaa
    10321 ggtcagcccc gcgacggccg tcgcgagggc cttgatcggc cccgtcttct cccgcgggag
    10381 gaagaggatc gccagcgccc cggccgtcgg gaggaggatg aggagggaga ggagtccgtt
    10441 cattcggcgt ccttacacgt cacgcgaaga tgatgtagat gcagacgagg gcgaagacgc
    10501 ccgcggccat cccgaatgcg tagttctgcg tctggcccga ctggagcttc ccgtagccct
    10561 tccaggccgc ctggaaggag tcgccgacgc cgttcaccgc accgtcgacg aacgtcctgt
    10621 cgaaccagtt cgaaacgtgg gagagcccca cggtgaggtg gcgcgtgccg ttcacgacgc
    10681 cgtcgacgac gcgggcgtcg atctcccaga ggagacgccc gcccttcttg acgagcccgt
    10741 tcacgaagac cgcctcgtac gcctcgtcga cgaagtactt gttccgcacc cagcgatagg
    10801 cgttcgggaa cgcggcgacg accttcgccg ggagcgccca gggcgccgac cccttgtaga
    10861 agaacgtcgt cgccaggagg atgccgaggc cggcgatcgc gaccgagccg agcatgaggc
    10921 cgtactcgag cgcgtgcgag gcctggtgcg ggtgcggcgt ccagtcgggc ggcagagccg
    10981 ggaaggccgg gtggaggaac gcctcgaagc ggttcccgcc cgggacgatg aagttcggga
    11041 tgccgacgta cccgacgacg accgcgccga ccgcgaggat ccagaggacg accgtcatcg
    11101 tcgccgggga ctcgtggacg tgctcccagg tctccttcgg cccccggtac tcgccgaaga
    11161 acgtcatctt gatcaggcgg aacatgtaga aggcggtcag gccggcggtg agcgccgcga
    11221 gcgcccagag ccacgggtgc ccctggagga aggtctcgcc gaggatcagg tccttcgaga
    11281 agaagccgga gaggagcggc acccccgaga tcgcgagcgt cgcgatgagc atcgtcctat
    11341 acgtgtgcgg gatcttcgag cggagcccgc ccatcgccgt catctcctgc tcccccgaga
    11401 gggcgtggat cacggagccc gagccgagga agaggcaggc cttgaagaag gcgtgggtga
    11461 agacgtggaa catccccgcc ccgaacgcga ggacgccgca gccgaggaac atgtagccga
    11521 gctgcgagac ggtcgagtag gcgaggacct tcttgatgtc ggtctgcgcg aggccgatcg
    11581 tcgcggccac gaacgccgtc actccgccga cgacggccac gacgaacgag gcgtcgtgcg
    11641 tcaggagccc ccagaccgag gccccctccc cgagcgtctt cagcgcccac tcgtgggaga
    11701 tccggaagag gacgttgcag cgaacgacca tgtagacgcc ggcggtgacc atcgtggcgg
    11761 cgtggatgag ggccgagacg ggcgtcgggc cggccatcgc gtcggggagc cagacgtaga
    11821 ggggaatctg ggcgctcttg cccgtcgccc cgacgaagag ggcgaggcag gcgaacgtca
    11881 cgaggccgcc cgccgcgtac gtggccgggg tcgcggcggc cgccgcgaac atcttcccga
    11941 agtcgaccgt cccgaacatc gcgacgcagg cgaagagccc catcaggaag ccccagtcgc
    12001 cgacgcggtt catgatgaag gccttcttgc cggcgtccgc cgcgaagtcc ttcgtgtagt
    12061 agtagccgat gaggaggtac gagcagaggc cgacgccctc ccagccgacg aagaggatcg
    12121 ccaggttcgc cccgaggacg agcgtcagca tcgcgaaca
//
LOCUS       DAOTEZ010000078        12151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3671_length_12151_cov_5.669064, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000078 DAOTEZ010000000
VERSION     DAOTEZ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12151
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3671_length_12151_cov_5.669064"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..1609
                     /locus_tag="P5164_06020"
     CDS             146..1609
                     /locus_tag="P5164_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013334391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRY43486.1"
                     /translation="MIDTSTAPRPLSLVVVGAGPAGLTAAWENVRHGGTATVLERDDV
                     VGGIARTVERRGYRFDIGGHRFFTKVREVEELWHEMLADDFLRVPRQSRILYRRKLFS
                     YPLKPFEAFFGLGPFESLRCGLSFLAARLAPRRPEEDFETWVSNRFGRRLFSIFFKTY
                     TEKVWGVPCTQIRADWAAQRIRGLSLWKAVWHAVKPDRSVKTLIEEFDYPRLGPGQMW
                     ERFTTRLEERGSRVLLGRSVARIGWQEGQGVTAVETDGSAGRETFTGDAYVSSMALSD
                     AVLALDPPPPPEVLEAARALRYRDYILVALMFEGERFFPDNWVYIHSPEVRIGRVQNF
                     GNWSAAMVPRPGVTCLGLEYFCFDTDPVWSAPDDELLETGRAEITKTGLVPEGARVLD
                     GAVVRMPKTYPMYDPGYSEKVDVVRRFFAERLPNLQQVGRNGMHRYNNQDHAMVTGLY
                     AVRNLYGASNDLWAVNVDDEYHEETRTLRPDEEGSRA"
     gene            complement(1682..3052)
                     /locus_tag="P5164_06025"
     CDS             complement(1682..3052)
                     /locus_tag="P5164_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HRY43487.1"
                     /translation="MLFKTIIEPFRIKSVESVKFTTREEREKALVEAGYNVFLVPAED
                     VLIDLLTDSGTGAMSSAQWGALMQGDESYAGSRSFFRFRDVIQDLTGFKHVIPTHQGR
                     AAERILFHTILKPGMVVPSNTHFDTTRANIEVEQAEARDLVIAEGKVPSLIHPFKGNV
                     DLAKLEKTLAEDGDRVPLVMVTVTNNSGGGQPVSLENLRGVRALCDKYGKPFFLDACR
                     FAENAWFVKQREAGQADRTPKAIAQEMFSLADGCTMSAKKDGLANIGGFLALNDDELA
                     ASCRSLLILTEGFPTYGGLAGYDLEAIARGLEEVVEEPYLRYRIRSTAYLADKLTAGG
                     VPIIQPPGGHAVYLDARAMLPHVAPLQYPGIAIANALYLEAGIRGVEIGTVMFGLHPD
                     GTEAPGPMDLVRLAIPRRVYTQSHIDLVAEGVLEVFRKREELRGYRITEAPKVLRHFS
                     ARFAPL"
     gene            complement(3067..3858)
                     /locus_tag="P5164_06030"
     CDS             complement(3067..3858)
                     /locus_tag="P5164_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43488.1"
                     /translation="MDAAAYSAFTDALFRRLDGDPRVVGLVALGSMSGDGPAPDDFSD
                     HDFFVVTRSGAQEAYRTDLVWLPGAERAVLVHRETEHGLKVILDDGHLLELAVFDLDE
                     LRVARANRYRVLLDRGGVAEGMAAIAAKPAESPTDRWLSGQLLGNLLVGGGRAARGEL
                     LSARLFVAGHATAHLLRLLSRHVPPEAPVPLDDLDPFRRFERASPRLGAELNAALSLP
                     PLAGALALLDLASRELGGRVAGWPAAAAETVRRRLSRMRDGGGAG"
     gene            complement(3953..5410)
                     /locus_tag="P5164_06035"
     CDS             complement(3953..5410)
                     /locus_tag="P5164_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HRY43489.1"
                     /translation="MTEKKSSTVRTILVVAAIAAFAAVGVTALLVNIMEHKQEARNPF
                     FRVVELDDDTEDPAVWGKNFPIQYDTYKRTVDMVRTKFGGSEALPRTPTEKDPRTQVT
                     TSKIEEDPRLKRMWLGYAFAVDFREERGHAYMLDDQTFTERQQVVKQPGTCMNCHAST
                     YVAMKKLGNGDIVKGFEAINQMPYFDARKHVKHPVACIDCHDSKTMALRVTRPAFMEG
                     IRAYKASQGVADYDVNTMATRQEMRTYVCGQCHVEYYFKGAEKRLTFPWHKGLKADEI
                     LAYYDEVGFKDWTHAETGAPNLKAQHPEFEMHNQGIHARSGVACADCHMPYTKVGAMK
                     VSDHHVRSPLLNINKACQTCHRWSEEELKARAETIQSRHMEMRNLAMDALMSLIDDLK
                     AAKEAGKSDEELAVARDFQRRASFLLDFAEAENSTGFHAPQEGARVLAQSIDASRKGQ
                     LAVRGALPATPQPLQKAGAPAAPKAPEKVALLSAR"
     gene            complement(5439..5936)
                     /gene="nrfH"
                     /locus_tag="P5164_06040"
     CDS             complement(5439..5936)
                     /gene="nrfH"
                     /locus_tag="P5164_06040"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HRY43490.1"
                     /translation="MRATDHPPHRRGAPRTRGLALAETAAGLLLGTALGIGAYTFVYA
                     KGASYLSNDPNACANCHVMTEQLDGWSRGSHRAVATCNDCHTPPGFVAKYATKASNGF
                     WHSFAFTSGRFPDPIQITDRNHRVTEKACRKCHADVVHDIDRVTREGRELSCISCHRS
                     VGHPH"
     gene            6113..7384
                     /locus_tag="P5164_06045"
     CDS             6113..7384
                     /locus_tag="P5164_06045"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HRY43491.1"
                     /translation="MTPRTLVLLALAVAPCAVAQEKPAAPPPPPAAAKSFDLGVQIRL
                     RGDATRDLDLAEESDDRDAWVLSRIRLFGTFRRGEKLAAHVELQDSRAFGSEASATSN
                     ERNVDLHQGWLRVGDAKDFLRLGRQEIAFGEQRLVGSFDWDNVGRSFDGALGRVTFEK
                     VPVVVDAFWAKVKESSGPPERSDVDFSGVHATWKSIPGIDLDTYVFVLRSGARDAAGD
                     DRLVTTIGARGHGSHGRLVWDAEGAIQTGHEFALDRRAWAVAGWIRYELPLPLKPSFG
                     LEASKATGDGDPLDGRFEEFDNLFPTNHLKYGYLDLTGWRNQRVVSLSAGVAGESVKW
                     TARLFCRALALDDPAGAWKAAGGAVLGLDATGSSGRSIGTELDVVGTYPILPGLTGQL
                     QAGWFRPGDYAKAVRGDDDALGASASLLFVF"
     gene            7448..8644
                     /locus_tag="P5164_06050"
     CDS             7448..8644
                     /locus_tag="P5164_06050"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY43492.1"
                     /translation="MPRRTSPTALLVVVGLAFFADSILYYLIVPLLPHYAKTLGLTQM
                     EVGVLFGVYAGALLAGTYPLGRLADRIGRRKPFLWGLLGLGATTFLFAFSSSYPLLLV
                     ARALQGLAAAATWTTGLALLAEGFPSEKRGQAMATVFAAANVGVLLGPPVSGLLTESL
                     GPRSPFLLAGAIVLADAVARVLLLGDDEPPRGERVGLRGLLADGTVRTLAGAMALGAA
                     LWAILESTLPLHLDAVLRAGPKEIGILFAVAALTHTVTSPVLGRLSDRVGRKKVLVVG
                     YVLAAFLVPVPAFLASKGAVGLSMAALGVTSSFVLSPVSPGLADAVERMGSRSFGGVF
                     SLVNIAYAVGMMGGPLVGSALVEAAGIRGALVATAALFALYLVPLARMGTSHRPSSAP
                     ATIPAP"
     gene            8641..9312
                     /locus_tag="P5164_06055"
     CDS             8641..9312
                     /locus_tag="P5164_06055"
                     /inference="COORDINATES: protein motif:HMM:NF015423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HRY43493.1"
                     /translation="MKPALPELTLAVEAAAVVVMTVSGMITAARARMDLIGTFTLALV
                     TAFGGGTLRDLILERRPLFWVANEGYVFGTLLLCLAFVYSRRAYATAAALDRKAVFVD
                     ALGLALFSLTGVRYALEAGLPPLPASLLGVVTGTFGGVLRDVLVREIPAIFRPGALYA
                     ASSFVGCWVVLSGPWLEVRQSFADAAGFATIVLLRMASVHLGLRLPDPHWVGKAGDGP
                     DGPTS"
     gene            complement(9302..10114)
                     /locus_tag="P5164_06060"
     CDS             complement(9302..10114)
                     /locus_tag="P5164_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HRY43494.1"
                     /translation="MKAIPLRTATVAPILLAALLAGGCSREEAPPAPAAAAGADLVGA
                     HGGGAAGTEVPSLRGTVLETMDAAGYTYLKLKTAEGETWAAVNEAKVAVGDEVTVLQP
                     MTMDGFESKTLNRTFERIVFGTLGPGAAAPASGMPGAAGSVPPEMAAAHAAAAAGPQG
                     DEKISVARAEGAEGRTIAELFAQRAALAGKPVAVRGKVVKVNLGIMGKNWFHLRDGSG
                     TAEGKNHDLTVTTQDAVSMGDVVLAKGIVAVDRDFGSGYTYSLLVEEAKVTK"
     gene            10294..12069
                     /locus_tag="P5164_06065"
     CDS             10294..12069
                     /locus_tag="P5164_06065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRY43495.1"
                     /translation="MPTRDLTLAMVGSGGDGVVTMGDMMAQAGARDGLNVIKVEAYGP
                     QIRGGESSCTLRMSADPISAQGDAVDVLVVFNWADFTRFKGEVVPKADAVILYEASDP
                     TPLEDVKIPTATNAAWIPVPFVELATKATGTKAAKNIVTLGILAELFGIPQESVRKAV
                     EFKFGKKKAGVLEANLKGFAAGREFAGTIGVDVSARKLEYSISAPKLVMTGNEACAVA
                     ALHAGCRFFAGYPITPSSEVLHFLSEWLPRSGGRIVQTEDELSALGAIIGGSFAGVKS
                     MTSTSGPGLSLMAEMLGLASMAEIPVVILDVQRGGPSTGLPTKSEQSDLYMAVFGSHG
                     DAPRIVLAPTDVEDCFHATVDAFNIAEETQLPVIVLSDQAIGQSRQTVEKANLVHEVI
                     DRRTPTAEELADYKRYAQTPTGVSPMSVPGTKGGMYQTNGLEHDETARPASSHVMHEK
                     MSRKRFKKLDFVEEKYPLYVHLGPEKADVGIVCWGSSKGPVEEAMKELAANGVSVSAF
                     VPRIIMPFPKKAFLEYAASVKTLVFLEVNFVGQLYNYLRTLVDLPAGTRVFTRSGGMN
                     LSVTEVKGRIESVLSEPGVREEVTA"
BASE COUNT         1619 a         4406 c         4233 g         1893 t
ORIGIN      
        1 cgacgcccgc ctcgggaaga cggccttccg gaagggctcg atctcgatct gagccggcgc
       61 ggagcgacgc cacgccgtgc gacactctcc aaggggcgtc gccgccggac cccggaccgg
      121 caggctcccg ggagagagca cacagatgat cgacacctcg accgccccgc gcccgctctc
      181 cctcgtcgtc gtcggcgccg ggccggcggg actcaccgcg gcctgggaga acgtccggca
      241 cggggggacc gcgaccgtcc tcgagcggga cgacgtcgtc ggcgggatcg cccggaccgt
      301 cgagcggcgc gggtaccggt tcgacatcgg cggccaccgg ttcttcacga aggtccgcga
      361 ggtcgaggag ctctggcacg agatgctcgc ggacgatttc ctgcgcgtcc cgcggcagtc
      421 ccgcatcctc tatcgccgga agctcttctc ctacccgctc aagccgttcg aagcgttctt
      481 cggtctcggc ccgttcgaga gcctccgatg cggcctcagc ttcctcgccg cccgcctcgc
      541 gccccggagg cccgaggagg acttcgagac gtgggtctcg aaccgcttcg gtcggcgcct
      601 cttctcgatc ttcttcaaga cctacaccga gaaggtctgg ggcgtcccct gcacgcagat
      661 ccgcgccgac tgggccgccc agcgcatccg cggcctctcg ctctggaagg cggtctggca
      721 cgccgtgaaa cccgacagga gcgtgaagac gctgatcgag gagttcgact acccgcgcct
      781 cggtccgggc cagatgtggg agcgcttcac gacccggctc gaggagcgcg gctcccgcgt
      841 cctcctcggt cggagcgtgg cacgcatcgg ctggcaggag gggcagggcg tcacggcggt
      901 cgagacggac ggctcggccg gacgggagac cttcacgggc gacgcctacg tctcctcgat
      961 ggccctctcc gacgccgtcc tcgcgctcga ccctcccccg ccgccggagg tcctggaggc
     1021 cgcccgcgct ctccgctacc gcgactacat cctcgtcgcg ctgatgttcg agggggagcg
     1081 cttcttcccc gacaactggg tctacatcca cagccccgag gtgcggatcg gccgcgtcca
     1141 gaacttcggg aactggtcgg ccgcgatggt cccccgcccc ggcgtcacgt gcctcggcct
     1201 cgagtatttc tgcttcgaca ccgacccggt ctggtccgcg cccgacgacg agctcctcga
     1261 gacgggccgg gcggagatca cgaagacggg cctcgtcccg gagggggccc gggtcctcga
     1321 cggcgccgtc gtgaggatgc cgaagaccta cccgatgtac gacccgggct actccgagaa
     1381 ggtcgacgtt gtccggcgct tcttcgcgga gcgcctcccg aacctccagc aggtcggacg
     1441 gaacgggatg caccgctaca acaaccagga ccacgcgatg gtgacgggtc tctacgcggt
     1501 gaggaatctc tacggcgcct cgaacgacct ctgggccgtc aacgtcgacg acgaatacca
     1561 cgaggaaacg cgaacgctgc gccccgacga ggaaggatcc cgggcgtagg ccgctccccg
     1621 cgccggcacc ccggcgcggg gtcgtctctc ccgatcccgc ggggtgcggc cccgcaccgg
     1681 ctcagagcgg cgcgaagcgg gccgagaagt gccgaaggac cttcggggcc tcggtgatcc
     1741 ggtagccgcg cagctcctcg cgcttccgga agacctcgag gaccccttcg gcgacgaggt
     1801 cgatgtgcga ctgcgtgtag acgcgccgcg ggatcgcgag ccggacgagg tccatcggcc
     1861 ccggcgcctc cgtcccgtcg gggtggaggc cgaacatcac cgtcccgatc tcgacgcccc
     1921 ggatccccgc ctcgaggtag agcgcgttcg cgatcgcgat gccggggtac tggagcggcg
     1981 ccacgtgcgg gagcatcgcc cgcgcgtcga ggtagaccgc gtgcccgccg gggggctgga
     2041 tgatcgggac gccgccggcc gtcagcttgt cggcgaggta ggccgtcgag cggatgcggt
     2101 agcgcaggta cggctcctcg acgacctcct cgaggccgcg ggcgatcgcc tcgaggtcgt
     2161 agccggcgag gccgccgtac gtcgggaagc cttccgtgag gatcaggagg ctgcggcacg
     2221 aggcggccag ctcgtcgtcg ttcagcgcga ggaagccgcc gatgttcgcg aggccgtcct
     2281 tcttggccga catcgtgcag ccgtccgcga gcgagaacat ctcctgcgcg atcgccttcg
     2341 gcgtccggtc ggcctgcccc gcctcgcgct gcttgacgaa ccaggcgttc tccgcgaagc
     2401 ggcaggcgtc gaggaagaag ggcttgccgt acttgtcgca gagggccctc acgccgcgga
     2461 ggttctcgag ggagacgggc tgcccgccgc ccgagttgtt cgtgacggtc accatcacga
     2521 gcgggacgcg gtcgccgtcc tcggcgagcg tcttctcgag cttcgcgagg tcgacgttcc
     2581 ccttgaaagg atggatcagc gaggggacct tcccctcggc gatgacgagg tcgcgggcct
     2641 cggcctgctc cacctcgatg ttcgcccggg tcgtgtcgaa gtgcgtgttg ctcgggacga
     2701 ccatcccggg cttgaggatc gtgtggaaga ggatgcgctc ggccgcccgc ccctggtgcg
     2761 tcgggatgac gtgcttgaac ccggtcaggt cctggatcac gtcgcggaag cggaagaacg
     2821 agcggctccc ggcgtagctc tcgtctccct gcatgagcgc gccccactgc gcggacgaca
     2881 tcgcgcccgt gccggagtcg gtcaggaggt cgatgaggac gtcctccgcc gggacgagga
     2941 acacgttgta tcccgcctcg acgagcgcct tctcgcgctc ttcgcgcgtc gtgaacttga
     3001 ccgattcgac cgacttgatg cggaaaggct cgatgatggt cttgaacagc atggcaaccg
     3061 gggattctac ccggcgccgc ccccgtcccg catcctggaa agcctcctcc ggaccgtttc
     3121 cgccgccgcg gccggccacc cggcgacgcg gcccccgagc tcgcgcgagg cgaggtcgag
     3181 gagggcgagg gcgcccgcga gcggcgggag cgagagcgcc gcgttcagct cggctccgag
     3241 ccgcggggag gcgcgctcga agcggcggaa cggatcgagg tcgtcgagag ggacgggagc
     3301 ctccggcggg acgtggcgcg agaggagccg gagcaggtgc gccgtcgcgt ggcccgcgac
     3361 gaagagacgc gccgagagga gctcgccccg cgccgcacgc ccccccccga cgaggaggtt
     3421 cccgaggagc tgcccagaga gccagcggtc cgtcggcgac tccgcaggct tcgcggcgat
     3481 cgccgccatc ccctccgcga cgcccccccg gtcgaggagg acgcggtagc ggttcgcccg
     3541 ggcgacgcgc agctcgtcga gatcgaagac ggcgagctcg aggaggtgcc cgtcgtcgag
     3601 gatcaccttc agcccgtgct ccgtctcgcg gtggacgagg accgcccgct ccgccccggg
     3661 aagccagacg aggtccgtcc ggtacgcctc ctgcgccccg ctccgggtca cgacgaagaa
     3721 gtcgtgatcg gagaagtcgt cgggcgccgg accgtcgccc gacatcgatc cgagcgcgac
     3781 gaggccgacg acgcgcgggt cgccgtcgag ccgccggaag agggcgtcgg tgaaggccga
     3841 gtaggcggcg gcgtccatgc cgcgattctc ccgcctccgg ggtccggagg gtcgaacggc
     3901 ccccgggtct ccccgggggc cgtctcggag acgaccgggt cgcccgtcgc cctcagcgcg
     3961 ccgagaggag cgccaccttc tccggggcct tcggcgcggc cggcgccccc gccttctgga
     4021 ggggctgcgg cgtggccggg agcgcaccgc ggacggcgag ctgtcccttg cgcgaggcgt
     4081 cgatcgactg cgcgaggacc cgggcccctt cctgcggcgc gtggaagccg gtggagttct
     4141 cggcctcggc gaagtcgagg aggaacgatg cccggcgctg gaagtcgcgc gcgaccgcga
     4201 gctcctcgtc gctcttcccg gcctccttcg ccgccttcag gtcgtcgatg agcgacatca
     4261 gggcgtccat ggccaggttc ctcatctcca tgtgccgcga ctggatcgtc tcggcccgtg
     4321 ccttcagctc ctcctccgac cagcggtggc aggtctggca ggccttgttg atgttcagga
     4381 gagggctcct cacgtggtgg tcggagacct tcatcgcccc gaccttcgtg tacggcatgt
     4441 ggcagtcggc gcacgccacg cccgaccggg catggatccc ctggttgtgc atctcgaact
     4501 cggggtgctg ggcctttagg ttcggggcgc cggtctccgc atgggtccag tccttgaacc
     4561 cgacctcgtc gtagtaggcg aggatctcgt cggccttcag ccccttgtgc cacgggaacg
     4621 tgagccgctt ctcggccccc ttgaagtagt actccacatg gcactggccg cagacgtacg
     4681 tccgcatctc ctggcgggtc gccatcgtgt tgacgtcgta gtcggcgacc ccctgggacg
     4741 ccttgtaggc ccggatcccc tccatgaacg cgggccgcgt cacgcggagg gccatcgtct
     4801 tcgagtcgtg gcaatcgatg caggcgaccg ggtgcttcac gtgcttgcgc gcgtcgaagt
     4861 acggcatctg gttgatcgcc tcgaagccct tcacgatgtc gccgttgccg agcttcttca
     4921 tcgcgacgta ggtggaagcg tggcagttca tgcaggtccc cggctgcttg acgacctgct
     4981 ggcgctccgt gaacgtctgg tcgtcgagca tgtaggcgtg gccccgctcc tcgcggaagt
     5041 cgaccgcgaa ggcgtagccg agccacatcc gcttcagccg cgggtcctcc tcgatcttcg
     5101 aggtcgtcac ctgcgtgcgc gggtccttct ccgtcggcgt cctcgggagc gcctcgctgc
     5161 cgccgaactt cgtccggacc atgtcgacgg tgcgcttgta ggtgtcgtac tggatcggga
     5221 agttcttccc ccagacggcc gggtcctccg tgtcgtcgtc cagctcgacc acgcggaaga
     5281 acgggttccg cgcctcctgc ttgtgctcca tgatgttgac gaggagcgcc gtcacgccga
     5341 cggccgcgaa cgcggcgatc gccgcgacca cgaggatcgt cctcacggtg ctgctcttct
     5401 tctcggtcat ctctctccct cgcttcccgt ggaggcgctc agtgcgggtg gccgacggac
     5461 cggtggcacg agatgcagga gagctcccgt ccctcgcgcg tcacccggtc gatgtcgtgg
     5521 acgacgtccg cgtggcactt ccggcaggcc ttctcggtca cgcggtggtt ccggtcggtg
     5581 atctggatcg ggtcggggaa ccgccccgac gtgaacgcga acgagtgcca gaagccgttc
     5641 gaggccttcg tcgcgtactt cgcgacgaag ccgggcggcg tgtggcagtc gttgcacgtc
     5701 gccacggccc ggtggctccc gcgcgaccag ccgtcgagct gctccgtcat gacgtggcag
     5761 ttcgcgcagg cgttcgggtc gttcgagagg taggaggcgc ccttcgcgta gacgaacgtg
     5821 taggcgccga tcccgagggc ggtcccgagg aggaggccgg ccgccgtctc ggcgagcgcg
     5881 aggccgcgcg tccggggcgc gccccgccgg tggggagggt ggtcggtcgc tctcacgccc
     5941 ggaactctcg cccggcgtcg ttcggccgtc aagcgcggag cggccactcc gtcctcgttc
     6001 ccccggtcgc catgacggac gtcatgcccg ggcgccggcc gacgatcgat ccttcgagcc
     6061 gcccggcggg gacgcctcga gcagcctgcc ggatcgccga aaggagtcgc gcatgacccc
     6121 gagaacgctg gtgcttctcg cgctcgccgt cgccccctgc gccgtcgcgc aggagaagcc
     6181 cgccgcgccc cctccgccgc ccgccgccgc gaagtcgttc gacctcggcg tccagatccg
     6241 gctccggggc gacgccaccc gcgacctcga cctggcggag gagagcgacg accgggacgc
     6301 ctgggtcctg tcgcgcatcc gcctcttcgg cacgttccgg cgcggggaga agctcgccgc
     6361 gcacgtcgag ctgcaggact cccgagcttt cggaagcgag gcgtcggcca cgtcgaacga
     6421 gcggaacgtc gacctgcacc agggctggct ccgcgtcggg gacgcgaagg acttcctgcg
     6481 gctgggccgg caggagatcg cgttcggaga gcagcgcctc gtcgggagct tcgactggga
     6541 caacgtcggt cggtccttcg acggggccct cggccgggtc acgttcgaga aggtcccggt
     6601 cgtcgtcgac gcgttctggg cgaaggtgaa ggagtcgtcc ggacctcccg agaggagcga
     6661 cgtcgatttc tcgggcgtcc acgcgacctg gaagtcgatt cccgggatcg acctcgacac
     6721 ctacgtcttc gtcctccggt cgggagcgcg cgacgcggcc ggggacgacc gcctcgtcac
     6781 gacgatcggc gcgcgcggtc acgggtccca cggccgcctc gtgtgggacg cggagggcgc
     6841 catccagacc gggcacgagt tcgcgctcga ccggcgcgcg tgggcagtgg ccggatggat
     6901 ccggtacgag ctcccgctcc cgctgaagcc ctcgttcggg ctcgaggcct cgaaggccac
     6961 cggcgacggg gacccgctcg acggccggtt cgaggagttc gacaacctct tcccgacgaa
     7021 ccacctgaag tacggctatc tcgacctgac cggctggcgg aaccagcgcg tcgtctcgct
     7081 ctcggccggc gtcgcgggag agagcgtcaa gtggacggcc cggctcttct gccgggcgct
     7141 cgccctcgac gacccggcgg gtgcgtggaa ggccgccggg ggcgccgtgc tcggcctgga
     7201 cgcgaccggc tcctcgggtc ggtcgatcgg aacggagctc gacgtcgtcg gcacctaccc
     7261 gatcctcccc gggctcaccg gccagctgca ggccggatgg ttccggccgg gcgactacgc
     7321 gaaggcggtc cggggagacg acgacgcgct cggcgcctcg gcgtcgctcc tcttcgtctt
     7381 ctgatctctc gccggggcgc gcccgatgag gcacgccccg gcaccgccgt gcagctacca
     7441 tcccggcgtg ccgcgccgca cctccccgac tgcgctcctc gtcgtcgtcg gcctcgcgtt
     7501 cttcgccgac tcgatcctct actacctgat cgtgccgctc ctgcctcact acgcgaagac
     7561 gctcggcctg acgcagatgg aggtgggggt gctcttcggc gtctacgcgg gcgcgctcct
     7621 cgcggggacc tacccgctcg ggcggctcgc cgaccggatc ggacggcgga agccgttcct
     7681 ctgggggctc ctcggactcg gggcgacgac gttcctcttc gcgttctcct cctcctatcc
     7741 gctcctcctc gtcgcgcgcg ctctgcaggg gctcgcggcc gccgccacct ggacgacggg
     7801 cctcgccctc ctcgccgagg gcttcccgtc ggagaagcgc ggccaggcga tggcgacggt
     7861 cttcgcggcg gcgaacgtcg gcgtcctcct cggccctccc gtctccggcc tcctgaccga
     7921 gagcctcggc ccgcgcagcc ccttcctcct cgccggcgcg atcgtccttg ccgacgccgt
     7981 ggcgcgggtc ctcctcctcg gcgacgacga gccgccccgc ggggagcggg tcggccttcg
     8041 ggggcttctc gcggacggaa cggtgcggac gctcgccgga gcgatggccc tcggtgcggc
     8101 cctctgggcg atcctcgagt cgacccttcc cctccacctc gacgccgtgc tccgggcggg
     8161 gccgaaggag atcgggatcc tcttcgccgt cgccgcgctc acgcacacgg tcacctcccc
     8221 ggtcctgggc cgcctctccg acagggtcgg gcggaagaag gtcctcgtcg tcggctacgt
     8281 cctcgccgcg ttcctcgttc cggtgccggc cttcctcgcc tcgaagggag cggtcggcct
     8341 ctcgatggcc gcgctcggcg tcacgagctc gttcgtcctg agccccgtca gccccggcct
     8401 cgccgacgcc gtcgaacgga tggggagccg ctccttcggc ggcgtcttct ccctcgtgaa
     8461 catcgcgtac gccgtcggga tgatgggcgg gccgctggtc gggagcgccc tcgtcgaagc
     8521 cgccgggatc cgcggggcgc tcgtggcgac cgctgcgctc ttcgcgctct acctcgtgcc
     8581 gctcgcccgg atggggacgt cgcatcgccc gtcctccgcc cctgcgacaa tcccggcacc
     8641 gtgaagcccg cgctccccga gctgacgctc gccgtcgagg cggccgccgt cgtggtgatg
     8701 acggtctccg ggatgatcac ggccgcccgc gcgcggatgg acctgatcgg gacgttcacg
     8761 ctcgcgctcg tcaccgcgtt cggtggcggg acgctccgcg acctgatcct cgagaggagg
     8821 cccctcttct gggtggcgaa cgaggggtac gtcttcggga cgctgctcct ctgcctcgcc
     8881 ttcgtctaca gccggcgggc ctacgccacc gccgcggcgc tcgaccggaa ggccgtcttc
     8941 gtcgacgccc tcggcctcgc cctcttctcc ctgacggggg tccgctacgc cctcgaggcg
     9001 ggtctccccc cgctccccgc gtcgctcctc ggcgtcgtca ccgggacgtt cggcggcgtc
     9061 ctgcgcgacg tcctcgtccg cgagatcccg gccatcttcc ggccgggcgc cctctacgcc
     9121 gcctcgtcgt tcgtcggctg ctgggtcgtc ctttcggggc cgtggctcga ggtgaggcag
     9181 tcgttcgccg acgccgcggg gttcgccacg atcgtcctcc tccggatggc ctcggtccac
     9241 ctcggcctgc gccttcccga cccgcactgg gtcgggaagg cgggcgacgg acccgacggg
     9301 cctacttcgt gaccttcgcc tcctcgacga ggaggctgta ggtgtagccc gacccgaagt
     9361 cgcggtcgac ggcgacgatc cccttcgcga ggacgacgtc gcccatcgac acggcatcct
     9421 gcgtcgtcac ggtcaggtcg tggttcttcc cttcggcggt cccggagccg tcgcgaaggt
     9481 ggaaccagtt cttccccatg atccccaggt tcaccttgac gaccttcccg cgcaccgcga
     9541 ccggcttccc tgcgagggcg gcgcgctgtg cgaagagctc cgcgatcgtg cgtccttccg
     9601 ccccctcggc cctggcgacg gagatcttct cgtccccctg gggaccggcc gcggccgcgg
     9661 cgtgcgccgc ggccatctcg ggcggaacgg agccggccgc tccgggcatg ccggacgcgg
     9721 gcgccgccgc accgggaccg agcgtcccga agacgatccg ctcgaacgtg cggttcagcg
     9781 tcttgctctc gaagccgtcc atcgtcatcg gctggaggac ggtgacctcg tcgccgacgg
     9841 ccaccttcgc ctcgttcacg gcggcccagg tctcgccttc ggccgtcttc agcttcaggt
     9901 aggtgtaacc cgcggcgtcc atcgtctcca ggacggtgcc gcggagcgag gggacctccg
     9961 ttccggccgc gccgccgccg tgcgcgccga cgaggtcggc tccggcagcg gccgcggggg
    10021 ccggcggcgc ctcctcgcgg ctgcagccgc cggcgaggag cgcggcgagg aggatcgggg
    10081 cgacggtcgc ggtacggagg gggatcgcct tcatgggcgc ggatggtacc gccagagggc
    10141 gcccgcgggg ccggggcctc ccggggaggc cggggtatag tgggctctgc ggcgcaggcc
    10201 ctgatgaaac ggcatcatgg cgcttcgcgc ccgacaggca gagactccag cgactttttc
    10261 ggggccgcga ggcggcccgt ggagggttcc gagatgccta cgagagacct cacgctcgcg
    10321 atggtcggct cgggcggcga cggcgtcgtg accatgggcg acatgatggc ccaggccggc
    10381 gcacgagacg gcctgaacgt catcaaggtg gaggcctacg ggccgcagat ccggggaggc
    10441 gagtcttcct gcacgctccg gatgtcggcc gatccgatct ccgcccaggg cgacgcggtc
    10501 gacgtgctcg tcgtcttcaa ctgggccgac ttcacccggt tcaagggcga ggtcgtcccg
    10561 aaggccgacg cggtcatcct ctacgaggcc agcgacccga cgccgctcga ggacgtgaag
    10621 atcccgacgg cgacgaacgc ggcgtggatc cccgtgccgt tcgtcgagct cgcgacgaag
    10681 gcgaccggga cgaaggccgc gaagaacatc gtcacgctcg gcatcctcgc cgagctcttc
    10741 ggcatcccgc aggagtcggt gcgcaaggcg gtcgagttca agttcgggaa gaagaaggcg
    10801 ggcgtcctcg aggcgaacct caagggcttc gccgccgggc gcgagttcgc cgggacgatc
    10861 ggggtcgacg tctctgcgcg gaagctggag tactcgatct ccgcgcccaa gctcgtcatg
    10921 acggggaacg aggcctgcgc cgtcgcggcg ctccacgcgg gctgccgctt cttcgccggc
    10981 tacccgatca ccccctcctc cgaggtcctc cacttcctct ccgagtggct cccccgctcc
    11041 ggcggccgga tcgtccagac cgaggacgag ctgtccgcgc tcggcgcgat catcggaggc
    11101 tccttcgccg gcgtgaagtc gatgacctcc acgtccgggc ccggcctctc cctcatggcc
    11161 gagatgctcg ggctcgcctc gatggccgag atcccggtcg tcatcctcga cgtgcagcgc
    11221 ggcggcccct cgacgggcct gccgacgaag tcggagcagt cggacctcta catggccgtc
    11281 ttcggatctc acggcgacgc gccgcgcatc gtcctcgcgc cgaccgacgt ggaggactgc
    11341 ttccacgcga cggtcgacgc cttcaacatc gccgaggaga cacagcttcc ggtcatcgtc
    11401 ctctcggacc aggcgatcgg gcagagccgg cagaccgtcg agaaggcgaa cctcgtccac
    11461 gaggtcatcg accgccggac cccgacggcc gaggagctcg ccgactacaa gcgctacgcc
    11521 cagaccccga cgggcgtctc cccgatgagc gtccccggga cgaagggcgg gatgtaccag
    11581 acgaacggcc tcgagcacga cgagaccgcc cggcccgcct cctcgcacgt catgcacgag
    11641 aagatgagcc ggaagcggtt caagaagctc gacttcgtcg aggagaagta cccgctctac
    11701 gtccacctcg gccccgagaa ggccgacgtc ggaatcgtct gctggggctc ctcgaagggc
    11761 cccgtcgagg aagcgatgaa ggagctcgcg gcgaacgggg tgagcgtctc cgcgttcgtc
    11821 ccgaggatca tcatgccgtt cccgaagaag gccttcctcg agtacgcggc gagcgtgaag
    11881 acgctcgtct tcctcgaggt gaacttcgtc gggcagctct acaactacct ccggaccctc
    11941 gtcgaccttc cggccgggac gcgcgtcttc acgcggtcgg gcgggatgaa cctctccgtg
    12001 accgaggtga agggccgcat cgagagcgtc ctctccgaac ccggcgtccg cgaggaggtg
    12061 accgcgtgag cagcgtcgtc gagacccccg ccccgccccg cttccagccg ggcgactaca
    12121 agagcgacct caagcccgtc tggtgcgccg g
//
LOCUS       DAOTEZ010000079        12142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3673_length_12142_cov_5.850914, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000079 DAOTEZ010000000
VERSION     DAOTEZ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12142
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3673_length_12142_cov_5.850914"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="P5164_06070"
     CDS             complement(<1..340)
                     /locus_tag="P5164_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY43496.1"
                     /translation="MSKHDVVVVGGGISGLSFAFEAGRAGRTVLVLEKDARPGGCLAT
                     RRAGSGYWFELGAHTCYNSYASFTTLLEACGLRSEVQARAKTHLRFLDGDRLVPGSNL
                     GVLLRLFSWPE"
     gene            488..1333
                     /locus_tag="P5164_06075"
     CDS             488..1333
                     /locus_tag="P5164_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43497.1"
                     /translation="MKNVLVRAVLAASFLLCLGSSTVALSQDDPKVLEGEAAFEHPST
                     QVVLKAAEHFRAGKVDAGMALYTASEQADWKKSSEKAELSALRKERAPDPKALAEGIK
                     KGGTLTLYGAEGQLRAPLPNRAMGIAYLALEKGAWRLTGGPMAIENAQEPAKEERFHG
                     DEMRAHPVYALALKYAETLHAKKDDSFMQLATKKAREKWATEPARERSESAAYRRRTI
                     PKKADLAASLKDGGYIVVEDDVRASLVLVSVTQSSPEPGTVSSTSSTLSLGFELEDGE
                     WRLAN"
     gene            complement(1426..2361)
                     /locus_tag="P5164_06080"
     CDS             complement(1426..2361)
                     /locus_tag="P5164_06080"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRY43498.1"
                     /translation="MRRHLVTALCLTLLAPAAFAQKAPKDVSELVARLDSSSASERAS
                     AARSIGYQKDAALPAVPRLLELLAGDRENEVRVSAAEALGNIGPKCAKTAVPALMKAA
                     KEDKWPKVRSASLSALGEMREAAKPAIPLLRESLRDPDGFIAQAARNALFRVQPDARD
                     EVVAIEDATRPKQKGSLFDDLTQLQAVLPGRAPEVYELAIYPGFALATTACAEAGSGR
                     CQLKYEGGAVTGPTDGSADDCEKKIALAKVDFSVVPGLVKQAPALLGAPGGAVTMVQL
                     SAGVFCKSHGWIVSVKDGGMVQFKLNGKVDKVTKF"
     gene            2739..3134
                     /locus_tag="P5164_06085"
     CDS             2739..3134
                     /locus_tag="P5164_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43499.1"
                     /translation="MKKSLCKVLSLAALALSFAAPAAANDSALFRLDQAVQVGSVALP
                     AGVYEFRVSDRGVVCVYDEEKAAVVGVTLARRDSLELAHLSGSATLTHDWAVRSLSIG
                     DFRYTFSPGAAPTATAALRQPTTVVALAR"
     gene            3286..4542
                     /locus_tag="P5164_06090"
     CDS             3286..4542
                     /locus_tag="P5164_06090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009645248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRY43500.1"
                     /translation="MPRPPSPRVVVHLDMDAFYAAVEVREDPSLLGKPVVIGHRGRRG
                     VVSTCSYEARRFGVRSAMPSVTAERLCPSAVWLPGRMSLYVEVSHRIREVLERYSPLV
                     EPLSIDEAFLDLTGIARDLSDGRRIAAEIKREIRDRERLTGSAGVAPNKFLAKVASDL
                     EKPDGLVVFPLEEVSTRLWPLPVERLWGVGPKTAERLRANGIARIADVASAGADRLAR
                     ILGEGSAEHLLALAHGRDDRVVQPGREAKSISEERTYGEDLTRPADVDKALLARADGV
                     ARELRHEGLVARTVHLKVRTGDFETVTRSRTLSEPTDLAEVLLEAARELFRDRIALRG
                     RGVRLLGVGVSGLEPAGAGQAALFGDERREKLRSLAKTADELREKFGRAALARARLIA
                     RPKTTGTDADAGEEDAAEASSLPSVD"
     gene            complement(4585..5517)
                     /locus_tag="P5164_06095"
     CDS             complement(4585..5517)
                     /locus_tag="P5164_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43501.1"
                     /translation="MSRRGLLLLLFAGILAAPPDAIGQPPPPGPVSPGGALRADGDVP
                     GAIAAFEKRYREDPGDRRNLLELAGALSINRRLDDCFRFLALAVRDAPTVAPLIDPDL
                     VTAREDPRWEVFEEALVARINAVPDPPIKDVAYARALWRLAAWDQAFFQEVGIAARKT
                     GTKSSVVEAIWKLKFLVQKRSQAELDSLVARKGWPRAGEVGKEAAMGAYLVAMHSSDG
                     AQKRYLPAIRAVCEAGELPWARYANMYDRSLFNDNLPQRYGTHTRFNERTKSEELYPL
                     EDESRVDEWRKEVGLPPLRDALAPLGIVFEPRRK"
     gene            complement(5528..6568)
                     /locus_tag="P5164_06100"
     CDS             complement(5528..6568)
                     /locus_tag="P5164_06100"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HRY43502.1"
                     /translation="MRLSRFVLGSLAAAASPLIAQVPPLQLPAASPKASVTQRVGMTD
                     VTVSYSRPAVKGRKVWGGLVPLGEVWRTGADENTAISFSSPVTVGGKTLPAGTYGLHT
                     IPGEKEWTVILSTQAKAWGSYSYDPGEDAVRFAAVPQEGPHEERLLFTLADPTDRSVA
                     VTLAWERLRLSFPVEVDTKAVVVASLKEQLRGLPRFGWQGWNGAAAWCVRNDTALDQA
                     EAWVERSIGMNRNFTNQSTKADLLEKKGDAKAAAQLREKAIATATEPELNQYGYQLLG
                     QKKVDDAIRIFEKNARDHPESWNAWDSLAEGQATAGDRKKAIANYEKALSMTGDEEQK
                     KRIRGEIAKLKP"
     gene            complement(6694..7371)
                     /locus_tag="P5164_06105"
     CDS             complement(6694..7371)
                     /locus_tag="P5164_06105"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HRY43503.1"
                     /translation="MITAAFAFPPSVTMELPGWELSFTELEPTGEGGAALGRARREVA
                     AAVRGRLRLETLSEHPAVAALRRLFRRAGCDPTRYRSGSEALLRRVLKGEDVPEVHPL
                     VDLGNLVALELATNVCVMDAEKAAPPFVFRAGRPGEEMLSLRGPFDCAGKPLLVDALG
                     PADVPITGADRVRVTEATRYAWLIVYLPAGVISPETASAAVQRLAGRTGVARLGRPTG
                     PENGHGA"
     gene            7461..7853
                     /locus_tag="P5164_06110"
     CDS             7461..7853
                     /locus_tag="P5164_06110"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRY43504.1"
                     /translation="MRYHLLVADEQERAEGRRGIERELKRGSLELIVLHLLAPAEAYG
                     YEIVSKLAAETSGALEVTDGTLYPVLYRLERAGFVSVRWETPQRGVPRKYYRLTDAGR
                     AELAALTLEWTTFARAMSRLLRTGRDAR"
     gene            7850..8443
                     /locus_tag="P5164_06115"
     CDS             7850..8443
                     /locus_tag="P5164_06115"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRY43505.1"
                     /translation="MTTANEFVERVLARLPDSVPDREQIGLELSSHIAERVARGTLLE
                     DVLRDLGDPTALAESYLSAVPLVSAPSLHRAVAKLVDLAIVLAAVALLATLAAALLPR
                     RLEGGIVLGALFTAILAFSAGTVFAELRFGQTPGKRIFGLRVVRESGAPIGLGQAIVR
                     QLPLVFSVFFVDAFFIFFTEKKQRAFEMLSKSRVVKV"
     gene            complement(8488..9510)
                     /locus_tag="P5164_06120"
     CDS             complement(8488..9510)
                     /locus_tag="P5164_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRY43506.1"
                     /translation="MRASWIRVLSLAAICSSGPAAGADGPPPPVEVGKIESARLGETR
                     EYWVSLPDDYRDGATRYPVVYMMDGELNFNSATIGGLRLAAQLGDIPDFIVVGIRNTD
                     RGRDVFPEVVTYGDGSKDGGRANEYLDFLREELIPRVEASYRTSGYRVLFGTSNTGFT
                     TVYALFRNPTIADAFVASSATLSVPSFREKRESLVKEFKGGRRRLSLVMGERDLPTVI
                     SQNGALKELIDTSAPAGLSCRLAVVRGAEHVPVDSLAAGLRDVFDGWKITERLTGETF
                     SALRAHAEGRLAKFGVAGDLPEGALKDLAESLLAEKKTAEAAEVLEYRARAYPTAAGA
                     PPPRAR"
     gene            complement(9532..10287)
                     /locus_tag="P5164_06125"
     CDS             complement(9532..10287)
                     /locus_tag="P5164_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRY43507.1"
                     /translation="MRVLVVDDEPLVRERLRALLAAHADVEVVGERGDGPAALQACRE
                     VAPDVVFLDIQMPGLSGLEVAETWRSEGALPVIVFVTAFDRYAVEAFRLHALDYLTKP
                     IEPGRFAESLDRAREYLEPRNRAALDERIRAMLEAHERRQAVRPHVVVRERERYLLVP
                     TAGIHALEAEGNYVSLHCERATHLLRDSLASVEAKLDPRRFLRTHRSWIVNLDFVKEA
                     RTRAHGSWVVVTRGGLSVPVGAQYRESLLKVLG"
     gene            complement(10287..11396)
                     /locus_tag="P5164_06130"
     CDS             complement(10287..11396)
                     /locus_tag="P5164_06130"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRY43508.1"
                     /translation="METRASLPRTGPADFLLSWDRLGFRILVFGGLGLMWVTQGVLYG
                     QPWPDLVRVELGTLMPWAFLAPLVLAWGKRVRIDRLGLGRFLAAHLLGMLVVFVPYWA
                     VRRGVAFAWDGFAAGWSASLVAGLLPTRQNLLGSFLGVPFAYFLILLGAEAMTHAQAR
                     REEEVQAERLAGQLAEARLALLRRQLHPHFLFNALQAVSTLLHRDPGAADGVLLRLST
                     LLRVMLETASGATVSLRTEVDLTRRYLEIEKVRFGDRLAFEWSVEESVLETPVPSLVV
                     LPLVENAIRHGLSAKVGPGRLEISARAEGASLVVTVEDDGLGAATPLRTGVGLANTRE
                     RLEGTYGARAGLEVDTAPGQGLRARLRVPLAEGRA"
     gene            complement(11440..>12142)
                     /locus_tag="P5164_06135"
     CDS             complement(11440..>12142)
                     /locus_tag="P5164_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43509.1"
                     /translation="DPARVPKLCEMADRQVARVRDYFTKRDPKKLESADWAAFEKSWK
                     TIQADARANPALVPPTITFEKEMTLHLDDVSVRLDFYGHAHGIADTIVRVPEENLVLT
                     AGVFYPTKVPTVDAVAEKATPAGIDNWFVVMRAVLSEANGETRFLPSHGRAVMKKGQY
                     EGYVSYLEGVWSGVKRARSGGRTLEQAKAELPLSSFPAIAKLPNEDLRGTEWENLDIH
                     GHNIERLWKLLDSPA"
BASE COUNT         1727 a         4454 c         4174 g         1787 t
ORIGIN      
        1 cctccggcca cgagaagagc ctcaggagga ctccgaggtt cgagccgggc acgaggcggt
       61 cgccgtcgag gaagcgaagg tgcgtcttcg ccctcgcctg cacctcggag cgcagcccgc
      121 aggcttccag gagcgtggtg aacgacgcgt aggagttgta gcaggtgtgc gcgccgagct
      181 cgaaccagta gccgctgccg gcacgccgcg tcgcgagaca gccgccgggc ctcgcgtcct
      241 tctcgaggac gaggaccgtc cttcccgccc ggcccgcctc gaacgcgaac gagagtccgc
      301 tgatcccgcc accgaccaca acgacgtcgt gcttgctcat gcggcgccga ggttcgcacg
      361 aaccggcagc gggcgcgagg gccgacgggg ggcggtcgga gtccgccacg gagcgcccaa
      421 gtgacgtgtc gtaagcgcct tgggagggat tctgcgagca cgtatcatcc acggaaagga
      481 gcccggaatg aagaacgtcc tcgttcgcgc ggtgctcgcc gcgtctttcc tcctctgcct
      541 cgggagctcg acggtcgccc tctcccagga cgacccgaag gtcctcgagg gcgaggcggc
      601 gttcgagcat ccctccaccc aggtcgtcct gaaggccgcc gagcacttcc gcgccgggaa
      661 ggtcgacgcg ggcatggcgc tctataccgc gtccgagcag gccgactgga agaagtcgag
      721 cgagaaggcg gagctctccg ccctgcggaa ggagcgcgcg cccgacccga aggcgctcgc
      781 cgaaggaatc aagaagggcg gaacgctgac gctctacggc gccgagggcc agctccgcgc
      841 tccgctcccg aaccgcgcga tggggatcgc ctacctcgcc ctcgagaagg gcgcgtggcg
      901 cctcacgggc ggcccgatgg ccatcgagaa cgcgcaggag ccggcaaagg aggagcgatt
      961 ccacggcgac gagatgcgcg cgcatcccgt ctacgcgctc gcgctgaagt acgccgagac
     1021 gctccacgcg aagaaggacg attcgttcat gcagctcgcc acgaagaagg cgcgcgagaa
     1081 gtgggcgacg gagcccgcgc gcgagcggtc cgagagcgcg gcctaccgcc gccgcaccat
     1141 tccgaagaag gccgacctgg ccgcgagcct gaaggacggc ggctacatcg tcgtcgagga
     1201 cgacgtccgc gcctcgctcg tcctcgtcag cgtcacgcag agctcgccgg agccagggac
     1261 cgtctcgtcc acctcctcga cgctctccct cggcttcgag ctcgaggacg gcgagtggag
     1321 actggcgaac tgaccttcgc gctcgcccac gccgaccctc gccggctccc cgggccggcg
     1381 agggccgcgg cgccctcctg attcacgggc gccgacggtg cgcgactaga acttcgtgac
     1441 cttgtcgacc ttcccgttca gcttgaactg gaccatcccg ccgtccttca cgctgacgat
     1501 ccagccgtgg ctcttgcaga agacgccggc gctcagctgg accatcgtca ccgctccgcc
     1561 cggcgcgccg agcagggcgg gcgcctgctt caccagcccc ggcaccacgg agaagtcgac
     1621 cttcgcgagc gcgatcttct tctcgcagtc gtccgcgctc ccgtcggtcg ggcccgtcac
     1681 ggcccctccc tcgtacttca gctggcagcg ccccgacccc gcttcggcgc aggccgtcgt
     1741 ggcgagcgcg aacccggggt agatcgcgag ctcgtagacc tccggcgcgc ggcccggcag
     1801 caccgcctgc agctgggtga ggtcgtcgaa gaggctcccc ttctgcttcg ggcgcgtcgc
     1861 gtcctcgatc gcgacgacct cgtccctggc gtccggctgc acgcggaaga gcgcgttccg
     1921 ggccgcctgc gcgatgaacc cgtccgggtc gcggagagat tcccggagga gcgggatcgc
     1981 gggcttcgcg gcctccctca tctcgccgag agccgagagc gacgcggagc ggaccttcgg
     2041 ccacttgtct tccttcgccg ccttcatgag cgccggcacc gccgtcttcg cgcacttcgg
     2101 cccgatgttg ccgagcgcct ccgcggccga gacgcgcacc tcgttctcgc ggtcgccggc
     2161 gaggagctca aggagccgag gcaccgccgg aagagcggcg tccttctggt agccgatcga
     2221 gcgggccgcg ctcgcacgct cgctcgcgct cgacgagtcg agccgcgcga cgagctccga
     2281 gacgtctttc ggcgccttct gcgcgaacgc ggcaggggcc agaagcgtca gacacagggc
     2341 cgtcacgagg tgacgtcgca tggggcctcc tcatcgggga cgaacgttcg ttccaagttt
     2401 ggcgggatgg tagcgcgtct ccacccggcg cccctcctcg agcgggcgcc gccccccggt
     2461 cgtgtgaact cctgtcacac ggaagagccc gaaacgaacc ttttcccggg tcgtccgtag
     2521 cagccgacag cccgctcggg aatgccggat cacccggcca cccggcgcgg ccagtcctcc
     2581 ggcccaggga ggtgtctcga gaaggtcgcg ggtgcgacga aacgacacag gattcggcag
     2641 ccgacaagaa ccccggcagc cctcgacccg agcgccgctc ggtgcgcggg gaccctgccc
     2701 ctacggaccc gggcaacgca caaacaggag gcattcgaat gaagaagtcc ctttgcaagg
     2761 tgctctcgct cgccgcgctc gcgctgtcgt tcgcggctcc cgctgccgcc aacgactccg
     2821 ccctctttcg tctggaccag gccgtccagg tgggcagcgt agcgctcccc gcgggcgtct
     2881 acgagttccg cgtctcggat cgcggggtcg tctgcgtcta cgacgaggag aaggccgcgg
     2941 tcgtcggcgt cacgctcgcg cggcgcgact ccctcgagct cgcccacctt tcgggctccg
     3001 cgacgctgac gcacgactgg gccgtccgct cgctctccat cggcgacttc cggtacacct
     3061 tctccccggg agcggcgccg accgcgacgg cggcccttcg gcagccgacc accgtcgtcg
     3121 ccctcgcccg gtagcgtccg cccgggaggg cggccgacga gcgcgctcct ccgcccacgg
     3181 cctcctcgtc ttcgcgagga ggccgcgggg cccgcggcgc atcgaggagc tgctcgcaga
     3241 cgctcgtcga accggctcgc tcctaccggc gctaagctcc ttcccgtgcc ccggccgccg
     3301 tcgccccgcg tcgtcgtcca cctcgacatg gacgccttct atgcggccgt ggaggtgcgc
     3361 gaggacccgt cgctcctcgg gaagcccgtc gtcatcgggc accgcggccg ccgcggcgtc
     3421 gtctccacct gttcgtacga ggcgcggcgc ttcggcgtcc gatcggcgat gccctcggtc
     3481 accgcggagc ggctctgtcc ctcggccgtc tggctccccg gtcggatgtc cctctacgtc
     3541 gaggtctccc accggatccg tgaggtcctc gaaaggtact cccccctcgt cgagccgctc
     3601 tcgatcgacg aggccttcct cgacctgacc ggcatcgcgc gcgacctctc cgacggccga
     3661 cgaatcgccg cggagatcaa gcgggagatc cgcgaccgag agcggctcac cggctcggcg
     3721 ggcgtggcgc cgaacaagtt cctcgcgaag gtcgcctcgg acctcgagaa gcccgacggc
     3781 ctcgtcgtct tcccgctcga ggaggtctcg acgcggctct ggccgctccc cgtcgagcgg
     3841 ctctggggcg tcggcccgaa gacggccgag cgccttcgcg ccaatggcat cgcgcggatc
     3901 gccgacgtcg cgagcgcggg cgcggaccgg ctcgcgcgca tcctcggcga agggagcgcc
     3961 gagcacctcc tcgccctcgc ccacggacgc gacgaccgcg tcgtgcagcc ggggcgcgag
     4021 gcgaagtcga tctccgagga gcggacctac ggcgaggacc tcacgcggcc ggcggacgtc
     4081 gacaaggccc tcctcgcacg ggcggacggc gtcgcccgcg agctacggca cgaagggctc
     4141 gtcgcccgca ccgtccacct caaggtgcgc accggcgact tcgagaccgt gacccgctcc
     4201 aggaccctct ccgagccgac ggatctcgcc gaggtcctcc tcgaggccgc gcgggagctc
     4261 ttccgggatc ggatcgcgct gagagggcgc ggcgtccgcc tcctcggcgt cggcgtcagc
     4321 ggcctcgagc ccgccggcgc gggccaggcc gccctcttcg gcgacgagcg ccgggagaag
     4381 ctccggagcc tcgccaagac cgctgacgag ctccgcgaga agttcggccg ggccgcgctc
     4441 gcgagggcga ggctcatcgc ccggccgaag acgaccggaa cggacgccga cgcaggcgag
     4501 gaggacgccg ccgaggcgtc gagccttccg agcgtcgact gagggcggaa gccggtacgg
     4561 ctgcgaggga tgtccggccc cgggctattt tctccggggc tcgaagacga tcccgagcgg
     4621 cgcgagggcg tcccggagcg ggggcagccc gacctccttc ctccactcgt ccacccggga
     4681 ctcgtcctcg agcgggtaga gctcttccga cttcgtccgc tcgttgaagc gcgtgtgcgt
     4741 gccgtaacgc tgcgggaggt tgtcgttgaa gaggctccgg tcgtacatgt tcgcgtaccg
     4801 cgcccagggc agctctccgg cctcgcagac ggcccggatc gcgggcaggt acctcttctg
     4861 cgccccgtcg ctcgagtgca tggcgacgag gtaggccccc atcgccgcct ccttccccac
     4921 ctcgccggcg cgcggccagc ccttcctcgc gacgagcgaa tccagctcgg cctggctccg
     4981 tttctggacc aggaacttga gcttccagat cgcctccacc acggaggact tcgtacccgt
     5041 cttccgggcc gcgatgccga cctcctggaa gaaggcctgg tcccaggccg cgagcctcca
     5101 gagcgcccgc gcgtaggcga cgtctttgat cggaggatcg ggaaccgcgt tgatccgggc
     5161 aacgagcgcc tcctcgaaca cctcccagcg cggatcctcc cgcgccgtca cgaggtcggg
     5221 gtcgatcagg ggggcgaccg tcggcgcatc cctgacggcg agggccagaa atcggaagca
     5281 gtcgtcgagg cgcctgttga ttgacagggc gccggccagc tcgaggaggt tccttcggtc
     5341 gccggggtcc tcgcggtacc tcttctcgaa ggcggcgatg gccccgggga cgtctccgtc
     5401 ggcccgaaga gccccgccgg gactcaccgg cccgggaggg ggcggctgac cgatcgcgtc
     5461 gggtggagcg gccaggatgc ccgcgaagag gaggagaagg aggcctcggc gggacatcga
     5521 cgatccccta cggtttcagc ttcgcgatct cgccgcggat ccgcttcttc tgctcctcgt
     5581 ccccggtcat ggagagcgcc ttctcatagt tcgcgatggc cttcttcctg tcgccggccg
     5641 tcgcctggcc ttcggcgagc gagtcccacg cgttccacga ttccgggtgg tccctcgcgt
     5701 tcttctcgaa gatccggatc gcgtcgtcca ccttcttctg cccgaggagc tggtagccgt
     5761 actggttcag ctcgggctcg gtcgcggtcg cgatcgcctt ctcgcgcagc tgggcggcgg
     5821 ccttcgcatc tcccttcttc tcgaggaggt cggccttcgt cgactggttc gtgaagttgc
     5881 ggttcatgcc gatcgagcgc tcgacccacg cctccgcctg gtcgagggcg gtgtcgttcc
     5941 tcacgcacca ggcggcggcg ccgttccacc cctgccagcc gaagcgcggc agcccccgga
     6001 gctgctcctt caggctcgcg accaccacgg ccttcgtgtc gacctccacc gggaaggaga
     6061 gccgcagcct ctcccaggcg agcgtcacgg cgaccgagcg gtccgttggg tccgcgagcg
     6121 tgaagaggag gcgttcttcg tgcggccctt cctgcgggac cgccgcgaag cgcacggcat
     6181 cctccccggg gtcgtagctg tagctccccc aggccttcgc ctgggtgctc agaatgaccg
     6241 tccactcctt ctcgcccgga atcgtgtgca gcccgtaggt gcccgcgggc agggtcttcc
     6301 cgccgacggt cacaggactc gagaacgaga tcgccgtgtt ctcgtcggcg ccggtccgcc
     6361 agacctcgcc gaggggaacg aggcctcccc agaccttccg gcccttcacg gcgggcctcg
     6421 aatagctcac ggtcacgtcg gtcatcccga ccctctgcgt caccgacgct ttcgggctgg
     6481 cggcggggag ctggagcgga gggacctggg cgatcagcgg agaagcggct gcggccaggg
     6541 agccgaggac gaatcgggac aggcgcatcg ggagtcctcc tgtcgaatcg gaatcgacgg
     6601 agcccgcatc cgactcccgg gctccgcccg agtgctgtgg acctctcctt cccgatacgt
     6661 cacccggccg ggcgggtttc cgggctcgag cggtcaggcg ccgtgcccgt tctccgggcc
     6721 tgtcgggcgg ccgaggcgcg ccacgccggt ccgcccggcg aggcgctgca cggccgcgga
     6781 ggccgtctcc ggggagatca cgccggcggg caggtagacg atgagccagg cgtatcgcgt
     6841 cgcctcggtc acgcgcaccc ggtcggcccc ggtgatcgga acgtcggcgg ggccgagcgc
     6901 gtcgacgagc agcggcttgc cggcgcagtc gaacggcccg cgcagagaca gcatctcctc
     6961 gcccggcctg ccggcgcgga agacgaacgg gggcgcggcc ttctccgcgt ccatgacgca
     7021 gacgttcgtg gcgagctcga gggccacgag gttcccgagg tcgacgagcg ggtggacttc
     7081 cggcacatcc tcccccttca gcacacgccg caggagcgcc tcggagcccg accggtagcg
     7141 cgtcgggtcg caccccgcgc ggcgaaagag gcggcggagc gccgcgaccg cgggatgctc
     7201 cgagagcgtc tcgagcctca ggcgcccgcg caccgcggcg gccacctccc gcctggcacg
     7261 acccagcgct gcgccgcctt cgccggtcgg ctcgagctcg gtgaaggaga gctcccatcc
     7321 cgggagctcc atcgtcacgg agggcgggaa cgcgaacgcc gccgtaatca cgacgccatt
     7381 ctcgccgcct tcgccgaggg cggcgcccct cggggatcct cgtcgagcgc tttcccttat
     7441 atcttgcgta accagatatc gtgcgttacc atttactcgt ggcggacgag caggagcggg
     7501 ccgaggggcg gaggggaatc gagagggagc tgaagcgtgg ctcgctcgag ctcatcgtgc
     7561 tccatctgct cgcgcccgcc gaagcgtacg ggtacgagat cgtctccaag ctcgcggccg
     7621 agacgagcgg agcgctcgag gtgaccgacg gcacgctcta cccggtcctc taccggctcg
     7681 agagggcggg tttcgtctcg gttcgctggg agacgccgca gcggggcgtc ccgcggaagt
     7741 actaccggct gaccgacgcg gggcgcgccg agctggccgc gctcacgctc gagtggacga
     7801 ccttcgccag ggccatgtcg agacttctcc ggaccgggag ggacgcccga tgacgaccgc
     7861 gaacgagttc gtcgagagag tgctcgcccg tctgcccgac tccgttccgg atcgggagca
     7921 gatcggcctc gagctgagca gccacatcgc cgagcgagtc gcccgcggca cgttgctcga
     7981 agacgtcctc cgggacctcg gcgacccgac ggccctcgcg gagtcgtacc tgagcgccgt
     8041 accgctcgtg agcgctccgt cgctgcaccg ggccgtggcg aagctcgtcg acctcgccat
     8101 cgtcctcgcc gccgtcgcgc tcctcgcgac gctcgccgca gcgctcctcc ctcgccgact
     8161 cgaggggggc atcgtcctcg gggcgctgtt cacggcgatt ctggccttct ccgccggcac
     8221 cgtcttcgcc gagctgcggt tcgggcagac cccgggcaag cgcatcttcg gccttcgcgt
     8281 cgtccgcgaa tccggggcgc cgatcgggct cggccaggcg atcgttcgcc agcttccgct
     8341 cgtcttctcg gtcttcttcg tcgacgcctt cttcatcttc ttcaccgaga agaagcagag
     8401 agccttcgag atgctctcga agagccgcgt cgtgaaagtc tgagccggat cgcctccggt
     8461 ccggctacgg gcgcccgccg cccctcttca ccgggcgcgg ggaggcggcg cacccgcggc
     8521 cgtgggatac gcccgggcgc ggtactcgag gacctccgcg gcctctgcgg tcttcttctc
     8581 ggcgaggagc gactcggcga ggtccttcaa cgcaccctcg ggcaggtcgc cggcgacgcc
     8641 gaacttcgcg aggcgccctt ccgcgtgcgc gcgaagcgcg gagaacgtct ccccggtgag
     8701 ccgctccgtg attttccagc cgtcgaagac gtcgcgcagc cccgccgcga ggctgtcgac
     8761 cggcacgtgc tccgctcccc ggacgacggc gagccggcac gacaggccgg cgggcgcgga
     8821 ggtgtcgatg agctccttca gggccccgtt ctggctgatg accgtcggga ggtcgcgctc
     8881 ccccatcacg agcgagagcc gccgccttcc tcccttgaac tccttcacga ggctctcgcg
     8941 cttctcgcgg aacgacggca ccgagagcgt cgcgctcgac gcgacgaagg cgtcggcgat
     9001 cgtggggttg cggaagaggg cgtagacggt cgtgaagccc gtgttcgagg tgccgaagag
     9061 cacccggtag ccgctcgtcc ggtacgacgc ctcgacccgg gggatcagct cttcccgtag
     9121 gaaatcgagg tactcgttcg cccgcccgcc gtccttgctc ccgtccccgt acgtcaccac
     9181 ctccgggaag acgtcgcggc cgcggtcggt gttccggatt ccgacgacga tgaagtcggg
     9241 gatgtcgccg agctgcgcgg cgagccggag cccgccgatc gtcgcggagt tgaagttcag
     9301 ctcgccgtcc atcatgtaga cgacggggta acgcgtcgcg ccgtccctgt agtcgtcggg
     9361 cagcgacacc cagtactcac gcgtctctcc cagccgcgcc gactcgatct tcccgacctc
     9421 caccggcggc ggcgggccgt cggcaccggc ggcagggccg gacgagcaga tcgccgccag
     9481 cgacaggacg cgaatccacg aagctctcat ctcgactcct tctcggttcg gctagcccag
     9541 gactttcagc agcgactcgc ggtactgcgc accgaccggg acgctcaggc cgccgcgggt
     9601 cacgacgacc cagctcccgt gggcccgcgt ccgggcctcc ttcacgaagt ccaggttgac
     9661 gatccaggag cggtgcgtgc ggaggaagcg acgcgggtcg agcttcgcct cgaccgaggc
     9721 gaggctgtcg cgaaggaggt gcgtcgcccg ctcgcagtgg agcgagacgt agttcccttc
     9781 cgcctccagc gcgtggatgc cggcggtcgg gacgagcaga tagcgctcgc gctccctcac
     9841 gacgacgtgc ggccgcaccg cctgccgccg ctcgtgagcc tcgagcatcg cccggatacg
     9901 ctcgtcgagc gccgcccggt tccgtggctc caggtactcc ctcgcccggt ccagcgactc
     9961 ggcgaatcgc ccgggctcga tcggcttcgt caggtagtcg agggcgtgca ggcgaaaggc
    10021 ctcgaccgcg taccggtcga aagccgtgac gaagacgatg acgggcaggg cgccctcgct
    10081 ccgccacgtc tccgccacct ccaggccgct cagccccggc atctggatgt cgaggaagac
    10141 gacgtccgga gcgacctcgc gacaggcctg cagcgccgcg gggccgtcgc cccgctcgcc
    10201 cacgacctcc acgtccgcgt gcgcggcgag gagcgcgcgg agacgctcgc gcacgagcgg
    10261 ctcgtcgtcg acgacgagga cgcgcatcac gcgcgcccct ccgcgagcgg gacgcggagc
    10321 cgggcgcgga gtccctgccc cggcgcggtg tcgacctcga gccccgcccg cgcgccgtac
    10381 gtcccctcga gccgctcgcg cgtgttcgcg aggccaactc ccgtccggag cggcgtcgcc
    10441 gccccgagcc cgtcgtcctc gacggtcacg acgagcgacg cgccctcggc gcgggccgag
    10501 atctcgagcc ggcccggccc caccttcgcc gagagtccgt ggcgaatcgc gttctccacg
    10561 agcggcagga cgaccaggct cgggaccggc gtctcgagca ccgattcctc gacgctccat
    10621 tcgaaggcga gccggtctcc gaacctcacc ttctcgatct cgaggtacct gcgggtcagg
    10681 tccacctccg tccggaggct cactgtcgcg ccggaggcgg tctcgagcat gacccgaagg
    10741 agggtcgaga gccgcaggag gacgccgtcg gccgccccgg gatcgcggtg gagaagcgtc
    10801 gagacggcct ggagcgcgtt gaagaggaaa tgcgggtgca gctgccttcg gaggagagcc
    10861 agccgggcct cggccagctg gcccgcgagg cgctcggcct ggacctcctc ctcccgccgg
    10921 gcctgcgcgt gcgtcatcgc ctccgcgccg aggaggatga ggaagtaggc gaacggcacg
    10981 ccgaggaagc tgccgaggag gttctggcgc gtcggaagga ggccggcgac gaggctcgcg
    11041 ctccatcccg ccgcgaagcc gtcccaggcg aaggcgactc cgcggcggac ggcccagtag
    11101 gggacgaaga cgacgagcat gccgagcagg tgggcggcga ggaagcgccc cagacccagc
    11161 cggtcgatcc ggacacgttt cccccacgcg aggacgagcg gcgccaggaa cgcccagggc
    11221 atgagcgttc ccagctcgac gcggacgagg tccggccagg gctggccgta gagcactccc
    11281 tgggtgaccc acatgaggcc gaggccgccg aagaccagga tgcgaaagcc caggcggtcc
    11341 cacgaaagga ggaagtccgc cggtccggtc cgcgggagag aggcccgtgt ctccatcttc
    11401 ggccttcggg tgatacgccg gccgccgatt cgggttccgt caggcgggcg agtccagcag
    11461 cttccagagg cgctcgatgt tgtgtccgtg gatgtcgagg ttctcccatt ccgtaccgcg
    11521 aaggtcctcg ttcgggagct tcgcgatcgc cgggaaggag ctcagcggca gctccgcctt
    11581 cgcctgctcg agcgtccgcc cgccggacct cgcgcgcttc acgccgctcc atacgccctc
    11641 gaggtacgac acgtagccct cgtactggcc cttcttcatg acggcccggc catggctcgg
    11701 caggaagcgc gtctccccgt tcgcctcgct cagcacggcg cgcatgacga cgaaccagtt
    11761 gtcgatgccc gccggggtcg ccttctccgc cacggcgtcc accgtcggca ccttcgtcgg
    11821 gtagaagacg ccggccgtca ggaccaggtt ctcctcgggg acccgcacga tcgtgtcggc
    11881 gatcccgtgc gcatggccgt agaagtcgag acgcacggag acgtcgtcga ggtggagggt
    11941 catctccttc tcgaacgtga tcgtcggcgg gacgagggcg gggttcgcgc gcgcgtccgc
    12001 ctggatcgtc ttccagctct tctcgaaggc ggcccagtcg gcgctctcga gcttcttcgg
    12061 gtcgcgcttc gtgaagtagt cccggacccg cgcgacctgc cggtccgcca tctcgcagag
    12121 cttcgggacg cgcgcggggt cg
//
LOCUS       DAOTEZ010000080        11915 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3767_length_11915_cov_5.705481, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000080 DAOTEZ010000000
VERSION     DAOTEZ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11915
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3767_length_11915_cov_5.705481"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="P5164_06140"
     CDS             complement(<1..542)
                     /locus_tag="P5164_06140"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HRY43510.1"
                     /translation="MTRTAVIAIGGNSLITDKEHQTVPDQYQAAAETCAHIAPLVAGG
                     DLRLVITHGNGPQVGFILRRSELASHELHMVPLDSCVADTQGALGYHIQLALGNELRR
                     LGVETGTATVVTQCLVDRSDPAFAKPNKPIGSFLTKEQAEHHREKDGWDVVEDAGRGW
                     RRVVASPAPKAILELDAVKSL"
     gene            complement(607..1932)
                     /gene="ssnA"
                     /locus_tag="P5164_06145"
     CDS             complement(607..1932)
                     /gene="ssnA"
                     /locus_tag="P5164_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HRY43511.1"
                     /translation="MRLLIRNGTLVTFGSPCRVLEGKALLLEGSRIARIAPAGEIPGP
                     WDREIDARGKLVLPGLVNAHMHFYSTLVRGLGKAAPSADFQQVLENLWWRLDRKLSLD
                     DVEVSAKVILVDAIRKGTTTLVDHHASPGAVTGSLGRIAKAVKETGLRSCLCYEVSDR
                     DGEKVAEEGLEENAAFARECASANDPQLRALFGLHAAFTLSDATLGKAARMGRDLGVG
                     FHVHVAEAASDVAANRERYGKSSARRLIDLGILGTGSIAAHAVHCDDADKDLLAASGA
                     WVVTNPQSNLNNAVGIADVVGLVRRGVPVGLGTDAMTVGMLEELRVGLWAQHLRQENP
                     SCGFLELTGALFARNPEIASALWGFPVGTLSEGAAADVILVDYLPPTPLHDDTVLGHL
                     VFGVSQAAVDTTICGGRVLMHGRRLEIGLDEEALAAESRRLATALWERF"
     gene            complement(1965..3422)
                     /locus_tag="P5164_06150"
     CDS             complement(1965..3422)
                     /locus_tag="P5164_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRY43512.1"
                     /translation="MSRIIKSIDTEVRKRTAKRCRERGIVIPTYAQMRDPKLLPESAK
                     KKLEGVGLWDVNPANLFRITWKNDHETGLFGGPNFLEIPREITGVKARIVGLVGKWFP
                     TGAHKVGAAFSCLVPRLVSGEFDPEKHKAVWPSTGNYCRGGAFDSAVLGCTAVAILPE
                     NMSQERFTWLRTIGSEVIATPGCESNVKEIYDKCWELKKDPVHVVFNQFDEMGNPIWH
                     YNVTGPAVEEAFQAVRGPKARLSAWVSATGSAGTIAAGDYLKTRFPGMKTVATEALQC
                     PTLLKNGFGDHRIEGIGDKHVPWVHNVRNTDAVAAIDDEDCMRLLRLFNEPAGRELLV
                     AAGAPADTVGKLDLLGISCICNILASIKAAKYWELDENDVVFTVFTDSAEMYLSRLAE
                     LTAERGAFTAIDAARTLTGSLERQATDNFKELTYQDRKAIHNLKYYTWVEQQGKTYEE
                     IQAQWNPEYWRELFEEEVVHFDRLIEEFNAEVGLG"
     gene            complement(3434..4627)
                     /gene="ygeW"
                     /locus_tag="P5164_06155"
     CDS             complement(3434..4627)
                     /gene="ygeW"
                     /locus_tag="P5164_06155"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005118265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HRY43513.1"
                     /translation="MPSQAIHDLAAEFKTFQNDLFQKDFLLTWEHSADQLKAILTLAE
                     TFKRLHQEGYSYRCFDTGLAISIFRDNSTRTRFSFASAASAMGLSLSDLDEQKSQIAH
                     GETVRETANMISFLTEVIGIRDDMFLGAGNSYMREVGDALTDGTKQGVLHRRPSLVNL
                     QCDVDHPTQSLADLAWLKKHFGGLENLKGKRIAMTWAYSPSYGKPLSVPQGIIGLMTR
                     FGMDVSLAYPEGYGLIPDVVEVARKNAAESGGKFEVVNSMEAAFANADIVYPKSWAPY
                     EVMGRRTELLNKGDKDGLKALEKECLANNAKFTSWECDRKKMDLTKGGKALYMHCLPA
                     DITDVSCKAGEVSAEVFEQYRIPTYHEAAYKPFVISALMFLERIADPAARLDEIVARG
                     RKLSI"
     gene            complement(4652..5998)
                     /locus_tag="P5164_06160"
     CDS             complement(4652..5998)
                     /locus_tag="P5164_06160"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRY43514.1"
                     /translation="MRTLVRNGRVAAAGRVESADLLLDGGLVSAVGRDLGEAERVIDA
                     SGCYVLPGLVDVHVHVGDRIGRFELADGYESGTRVAVKNGVTTLCAFVTQGPGQSLGE
                     ALRAARARAAGECHGDVAWHLTPTRFEETDWAEMEGLARAGYRTFKLYTTYRPAGLFS
                     DLGRLESVFRRLGPLGVRFLLHCEDDGLLGSVDASALDLSRASAHARLRPEGAEVAAV
                     EALLALAARHGVPLHVVHVSTVGAAELIRDARARQDVTCETGPQYLWLDETWLDRPDG
                     HRWLCSPPLRGDRERFRQLAREGAFDLFATDHCAFRKSDKDDWDGRDVRTVANGLPGL
                     GGLLHHAWRVFADDPDRAAAEVSLRLAKNPAARLGLEGRKGSLREGLDADVVVLDPNG
                     PERPVRATESDAYEPFPGFTSTLALRHVFVHGEPVVKDGDLVDPAVRRGQLHPNHD"
     gene            complement(6027..6905)
                     /locus_tag="P5164_06165"
     CDS             complement(6027..6905)
                     /locus_tag="P5164_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43515.1"
                     /translation="MKRLARILPAAAALLLLAPAATAADWTDMFVGYRYGYHVNDPAI
                     DHDIIKNVAQFTVANRYALGSNFLNVDVLMSNSYDPANGATSGGAQEIYVTYRHQLSL
                     NKAFKAGIKSKLVRDVAVTVGGDLNSKNTGFAPGKRMIVVGPTVNLNPKKGFADLGVW
                     YYKEWTHNAFAATGQKDVEFDGTVMFNLTWGLPIALSKEVSSTFKGFAYATLPKGKDA
                     AGVETETEVLSRMTWQFDVGALVGIRKGTISMGPGYELWYHKFGNPSPIPVPGSTATK
                     PNHTTSAPTFQAEIHF"
     gene            complement(6935..8335)
                     /locus_tag="P5164_06170"
     CDS             complement(6935..8335)
                     /locus_tag="P5164_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HRY43516.1"
                     /translation="MATNNGVHPVDEVLPPARLTLLGLQHVLVMYAGAVAVPLIVGGA
                     LKLPKEQMAMLINADLFACGIATLVQSIGLGRFGIRLPVMMGVTFAAVSPMVVMATNP
                     EIGLLGIYGAVIVAGLFGILVAPLISRMLPLFPPVVTGSIITIIGISLMRVGVNWAAG
                     GNPMIKNPADMAGPMIPNPAYGAPLHLAVAGIVLVFILLVTKFVKGFFANIAVLLGLV
                     LGMVIAIALGKVDFGGLASEKWFAVVYPFQFGVPTFDFVACLTMCMVMVVVMIESLGM
                     FLAVGNIVGKEIDQKHLSDGLRVDGLGTLIGGVFNTFPYTSFSQNVGLVGVTGVRSRW
                     VTAVGGVLLLALGLVPKLAFVVASVPQFVLGGAGIVMFGMVCATGIRILGGVDFAKNR
                     LNLYVVALAIGFGMIPLVADRFFAQMPKALSPLLHSGILLCSIVAVALNAFFNHLKGA
                     GEAASEVEKAAAKAEA"
     gene            complement(8350..11676)
                     /gene="ygfK"
                     /locus_tag="P5164_06175"
     CDS             complement(8350..11676)
                     /gene="ygfK"
                     /locus_tag="P5164_06175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HRY43517.1"
                     /translation="MEKAFRPLPLELLARWVFGELDARETVLGVPKGSFARPEPRFSS
                     RLLGRELAAPVGVAAGPHSQLAQNIVAAWLCGARFVELKTVQVLDELEISRPCIDSAD
                     ETFNCEWSQELRLEQSFDEYLKAWVLVHALAAKLSLPAPGTLFAMSVGYNLEGIRSER
                     VQRFIASMRDASGSIPSAVEAVAKAWPGVRDVAIPASISDHITLSTMHGCPPAEIEKI
                     ALYLLRELGVHTWVKLNPTLLGPERLRGLLNETFGHGVEVPDAAFGHDPRFEDALPMV
                     KRLAAAARAAGREFGVKLSNTLEVVNHRPVFPASEKMMYMSGRALHPLTLSLAKLVDD
                     ELDGSVPISFCGGADAWNFADLVSDGMAPVTVCTDLLKPGGYARLSQYLDNLGAAMDA
                     AGAESLDAFVEKTSGGKGRRWNLARHVERVVADGRRGRFARRERPLAFKGGRALGPFD
                     CIAAPCMEACPAHQNIPDYLWLVANGRETEAIEVIRRSNALPGVTGSICDHPCTERCV
                     RNLYDAPLGIREIKRFAFENGVSKPEVAGEAKGVRVAIVGAGPAGLSAALNLARAGIA
                     SEVFEAKGHAGGMVGGAVPRYRLDDGKLAVDLERLRSLGVAIHLNTAVGKDVSLDDLR
                     ARFDYVFLGAGAQKGKRLGIPGEDAEGVLDALVLLDGLREGARRDLGKKVLVVGGGNS
                     AMDGARSAKRLVGAGGEVSLVYRRTRAEMPADPEEVHDCEVEGIGLKTLLAPVRVVVE
                     NGKAVGLACQAMRLGPPDASGRPRPIPVEGSEVVLPADTIVTAIGQETALEFLGGAAL
                     EAKKDGTLVAKAATGETSTAGLFAGGDVVRGPASIIKAVADGRAAADAIARLHGVEPA
                     AEPHLPKGKTLPALMEKKARLARPAEVPVLPPDRRGGFDEVLQSLPADAARAEAARCL
                     DCDEACSLCVTVCPNRANLAYETSPVALELPRFVVEGGRPVPSGTTTFAVEQGVQIVN
                     LGDACNQCGNCVPFCPTAGAPWQEKPTFWVDAEGYAEAKGDAYRMTRAGDGVAIDARI
                     GGRQHRLARHGGLAEYRSDELRVVLETGAWRVVDVRLVGEPAEGALLDLSACARLVAL
                     LAAEPALPPLPESKPDGAAV"
BASE COUNT         1660 a         4010 c         4418 g         1827 t
ORIGIN      
        1 aggctcttca cggcgtcgag ctcgaggatc gccttcggag cgggcgaggc gacgacgcgg
       61 cgccagccgc ggccggcgtc ctcgacgacg tcccagccgt ccttctcccg gtggtgctcc
      121 gcctgctcct tcgtcaggaa cgagccgatc ggcttgttcg gcttcgcgaa ggcggggtcg
      181 gaccggtcga cgaggcactg ggtcacgacc gtcgccgtcc cggtctccac gccgagtcgc
      241 cggagctcgt tcccgagggc gagctggatg tggtagccga gcgcgccctg cgtgtccgcg
      301 acgcacgagt cgagcggcac catgtggagc tcgtgcgagg cgagctcgga ccgccggagg
      361 atgaagccga cctgcggacc gttgccgtgc gtgatgacga ggcgcaggtc gccgccggcc
      421 acgagcggcg cgatgtgcgc gcacgtctcc gccgcggcct ggtactggtc gggcaccgtc
      481 tggtgctcct tgtcggtgat gagggagttg cctccgatgg cgatgacggc cgtacgggtc
      541 aagggagcct cctgtcggcg cagcggaccg ccgcgcgcgt cgtggtgtga tgggagggaa
      601 acggcctcag aaccgctccc agagggctgt ggcgaggcgg cggctctcgg ccgcgagggc
      661 ctcctcgtcg aggccgatct cgagcctgcg gccgtgcatc aggacccgcc cgccgcagat
      721 cgtcgtgtcg acggcggcct gcgagacgcc gaagacgagg tggccgagga cggtgtcgtc
      781 gtggagcggc gtcggcggga ggtagtcgac gaggatcacg tcggccgcgg cgccctcgga
      841 gagcgtcccc acggggaatc cccagagggc cgaggcgatc tcggggttcc tcgcgaagag
      901 cgccccggtc agctccagga agccgcagct cgggttctcc tgcctgaggt gctgcgccca
      961 gaggccgacg cggagctcct cgagcatccc gacggtcatc gcgtcggtcc cgaggccgac
     1021 gggcacgccc cggcggacga gcccgacgac gtcggcgatg ccgacggcgt tgttgaggtt
     1081 cgactggggg ttcgtgacga cccaggcgcc gctcgcggcg aggaggtcct tgtccgcgtc
     1141 gtcgcagtgg acggcgtggg ccgcgatgct ccccgtcccg aggatcccga ggtcaatcag
     1201 ccgccgcgcg gacgacttgc cgtaccgctc ccggttcgcg gcgacgtcgg aggccgcctc
     1261 ggcgacgtgg acgtggaacc cgacgccgag gtcgcgcccc atccgggcgg ccttcccgag
     1321 cgtcgcgtcg gagagcgtga aggcggcgtg gaggccgaag agggcgcgga gctgcgggtc
     1381 gttcgccgag gcgcactcgc gcgcgaacgc ggcgttctcc tcgagcccct cttcggcgac
     1441 cttctccccg tcccggtccg acacctcgta gcagaggcag gagcggagcc ccgtctcctt
     1501 caccgccttc gcgatcctcc cgagggagcc ggtcaccgcg cccgggctcg cgtggtggtc
     1561 gacgagggtc gtcgtcccct tccggatggc gtccaccagg atcaccttcg cgctcacctc
     1621 gacgtcgtcg agggagagct tccggtcgag gcgccaccag aggttctcga ggacctgctg
     1681 gaagtcggcg ctcggcgcgg ccttcccgag gccgcgcacg agcgtcgagt agaagtgcat
     1741 gtgggcgttg acgaggccgg ggaggacgag cttcccgcgg gcgtcgatct cgcggtccca
     1801 cgggccgggg atctccccgg cgggcgcgat gcgcgcgatg cgactcccct cgaggaggag
     1861 ggccttcccc tcgaggaccc ggcaggggct cccgaaggtg acgagcgtgc cgttccggat
     1921 caggagccgc atcacgtcct ccgtctttcc gccggtcccc ggcgtcagcc gaggccgacc
     1981 tcggcgttga actcctcgat gagccggtcg aagtggacga cctcctcctc gaagagctcg
     2041 cgccagtact ccgggttcca ctgcgcctgg atctcctcgt aggtcttccc ctgctgctcg
     2101 acccaggtgt agtacttcag gttgtggatc gccttgcggt cctggtaggt cagctccttg
     2161 aagttgtccg tcgcctgccg ctcgaggctc cccgtgagcg ttcgggccgc gtcgatcgcc
     2221 gtgaacgccc cgcgctccgc cgtcagctcc gcgaggcgcg agaggtacat ctcggcgctg
     2281 tcggtgaaga cggtgaagac gacgtcgttc tcgtcgagct cccagtactt cgcggccttg
     2341 atcgaggcca ggatgttgca gatgcacgag atcccgagga ggtcgagctt cccgaccgtg
     2401 tcggcgggcg ccccggccgc cacgaggagc tcccggcccg ccggctcgtt gaagagccgg
     2461 aggaggcgca tgcagtcctc gtcgtcgatc gcggcgacgg cgtcggtgtt cctcacgttg
     2521 tggacccacg gcacgtgctt gtcgccgatc ccctcgatcc ggtggtcgcc gaacccgttc
     2581 ttcaggaggg tcgggcactg gagcgcctcg gtcgcgacgg tcttcatgcc ggggaacctc
     2641 gtcttcaggt agtcccccgc ggcgatcgtc ccggcgctgc ccgtggcgct gacccaggcc
     2701 gagaggcgcg ccttcggccc gcggaccgcc tggaaggcct cttccacggc gggcccggtc
     2761 acgttgtagt gccagatcgg gttccccatc tcgtcgaact ggttgaagac gacgtgcacc
     2821 gggtccttct tcagctccca gcacttgtcg tagatctcct tcacgttgct ctcgcagccc
     2881 ggcgtcgcga tgacctcgga gccgatggtg cggagccacg tgaagcgctc ctgggacatg
     2941 ttctcgggga ggatcgcgac cgccgtgcag ccgaggacgg ccgagtcgaa ggccccgccg
     3001 cggcagtagt tccccgtcga cggccagacg gccttgtgct tctccgggtc gaactcgccg
     3061 ctgacgaggc gcggcacgag gcacgagaac gccgccccga ccttgtgggc ccccgtcggg
     3121 aaccacttcc cgacgaggcc gacgatccgg gccttcaccc cggtgatctc cctcgggatc
     3181 tcgaggaaat tcggtccgcc gaagaggccc gtctcgtggt cgttcttcca ggtgatccgg
     3241 aagaggttcg cggggttgac gtcccagagg ccgaccccct cgagcttctt ctttgcgctc
     3301 tcgggaagga gcttcgggtc ccgcatctgc gcgtacgtcg ggatgacgat cccgcgctcg
     3361 cggcagcgct tcgccgtccg cttcctcacc tcggtgtcga tcgacttgat gatgcgcgac
     3421 atggcgcgcc tcctcagatg ctcagcttcc ggccgcgggc gacgatctcg tcgagcctgg
     3481 cggccgggtc ggcgatccgc tcgaggaaca tgagcgcgct gatgacgaac ggcttgtagg
     3541 ccgcctcgtg gtacgtcggg atccggtact gctcgaagac ctcggcgctc acctcgcccg
     3601 ccttgcagct gacgtcggtg atgtcggccg ggaggcagtg catgtagagc gcctttcccc
     3661 ccttcgtcag gtccatcttc ttccggtcgc actcccagct cgtgaacttc gcgttgttcg
     3721 cgaggcactc cttctcgagc gccttcaggc cgtccttgtc ccccttgttc aggagctcgg
     3781 tccgccggcc catgacctcg tacggggccc acgacttcgg gtagacgatg tccgcgttcg
     3841 cgaaggccgc ctccatcgag ttgacgacct cgaacttccc gccgctctcc gccgcgttct
     3901 tcctcgcgac ctcgacgacg tccgggatca ggccgtagcc ctcggggtag gcgaggctca
     3961 cgtccatccc gaagcgggtc atcaggccga tgatcccctg cggcacggag aggggcttgc
     4021 cgtagctcgg gctgtaggcc caggtcatgg cgatccgttt ccccttcagg ttctcgaggc
     4081 cgccgaagtg cttcttcagc caggccaggt cggcgagcga ctgcgtcggg tggtcgacgt
     4141 cgcactggag gttgacgagg ctcgggcggc ggtggaggac gccctgcttc gtaccgtcgg
     4201 tgagggcgtc gccgacctcg cgcatgtagg agttccccgc gccgaggaac atgtcgtcgc
     4261 ggatgccgat gacctcggtg aggaacgaga tcatgttggc cgtctcgcgg acggtctcgc
     4321 cgtgggcgat ctggctcttc tgctcgtcga ggtcggagag cgagaggccc atcgcgctcg
     4381 cggccgaggc gaacgagaag cgcgtgcggg tggagttgtc gcggaagatg gagatcgcga
     4441 ggcccgtgtc gaagcagcgg taggagtacc cctcctggtg gagccgcttg aacgtctcgg
     4501 cgagcgtcag gatcgccttg agctggtcgg ccgagtgctc ccaggtcagg aggaagtcct
     4561 tctggaagag gtcgttctgg aaggtcttga actcggcggc gaggtcgtgg atggcctggg
     4621 acggcatggc tctctccttt tccttcttct ctcagtcgtg gttcgggtgg agctgtcccc
     4681 tgcggaccgc cgggtcgacg aggtcgccgt ccttcacgac gggctccccg tggacgaaga
     4741 cgtggcgcag ggcgagtgtc gaggtgaagc ccgggaacgg ctcgtacgcg tcgctctcgg
     4801 tcgcccggac gggccgttcg gggccgttcg ggtcgaggac gacgacgtcg gcgtcgaggc
     4861 cctcccgcag cgaccccttt cggccctcga ggccgaggcg ggcggccggg ttcttcgcga
     4921 ggcggagcga gacctccgcg gcggcgcggt ccgggtcgtc cgcgaagacg cgccaggcgt
     4981 ggtggaggag gccgccgagg cccggcaggc cgttcgcgac cgtcctcacg tcgcggccgt
     5041 cccagtcgtc cttgtcggac ttccggaagg cgcagtggtc cgtggcgaag aggtcgaacg
     5101 ccccctcgcg ggcgagctgg cggaagcgtt cgcggtcgcc gcggagcggc ggcgagcaga
     5161 gccagcggtg cccgtcgggg cggtcgagcc acgtctcgtc gagccagagg tactgcggtc
     5221 ccgtctcgca ggtgacgtcc tgccgcgccc gggcgtcgcg gatgagctcg gcggcgccga
     5281 cggtcgagac gtggacgacg tggagcggga cgccgtgccg cgcggcgagg gcgaggagcg
     5341 cctcgaccgc ggccacctcc gctccctcgg ggcggaggcg ggcgtgcgcc gaggccctcg
     5401 agaggtcgag cgcggacgcg tcgacggagc cgaggaggcc gtcgtcctcg cagtggagga
     5461 ggaagcggac gccgagcggc ccgaggcggc ggaagacgga ctcgaggcgg ccgaggtcgg
     5521 agaagaggcc ggccgggcga taggtcgtgt agagcttgaa ggtgcggtac cccgcccgcg
     5581 cgagcccctc catctccgcc cagtcggtct cctcgaaccg cgtcggcgtc aggtgccagg
     5641 cgacgtcgcc gtggcactct cccgccgccc gcgcgcgggc cgcccggagc gcctcgccga
     5701 gcgactgccc cggcccctgc gtgacgaacg cgcagagcgt cgtgacgccg ttcttcacgg
     5761 cgacgcgcgt cccgctctcg tagccgtccg cgagctcgaa gcggccgatc cgatcgccga
     5821 cgtggacgtg cacgtcgacg agcccgggga ggacgtagca gcccgaggcg tcgatgaccc
     5881 tctcggcttc gccgaggtcg cggccgacgg cggagacgag ccccccgtcg aggaggaggt
     5941 ccgcgctctc gacgcggccc gccgcggcca cccggccgtt cctgacgagc gttctcatcc
     6001 gcgcctcccg tcctctccgt cccgcgtcag aagtggatct cggcctggaa ggtcggggcg
     6061 gaggtcgtgt ggttcggctt cgtggccgtc gagcccggca ccgggatcgg cgacgggttg
     6121 ccgaacttgt ggtaccagag ctcgtagccc gggcccatgg agatcgtccc cttcctgatc
     6181 ccgacgagcg cgccgacgtc gaactgccag gtcatccggg agaggacctc cgtctccgtc
     6241 tcgacgcctg cggcgtcctt gcccttgggg agcgtcgcgt aggcgaagcc cttgaaggtc
     6301 gaggagacct ccttgctcag cgcgatcggg aggccccacg tcaggttgaa catcacggtg
     6361 ccgtcgaact cgacgtcctt ctggcccgtc gcggcgaagg cgttgtgggt ccactccttg
     6421 tagtaccaga cgccgaggtc ggcgaagccc tttttcgggt tcaggttcac cgtcgggccg
     6481 acgacgatca tcctcttccc gggcgcgaac cccgtgttct tcgagttgag gtcgccgccg
     6541 accgtgaccg cgacgtcgcg gacgagcttg ctcttgatgc ccgccttgaa ggccttgttg
     6601 aggctgagct ggtggcggta ggtgacgtag atctcctggg cgccgcccga ggtcgccccg
     6661 ttcgccgggt cgtacgagtt cgacatcagg acgtcgacgt tcaggaagtt gctcccgagc
     6721 gcgtagcggt tcgccaccgt gaactgggcg acgttcttga tgatgtcgtg gtcgatcgcc
     6781 gggtcgttga cgtggtagcc gtaccggtag ccgacgaaca tgtcggtcca gtcggccgcg
     6841 gtcgcggcgg gggcgagaag gagcagggcc gcggcggccg gcaggatcct cgcgaggcgc
     6901 ttcatggtgt ctctcgtcct ctcccggccg ggcctcacgc ctcggccttc gcggcggcct
     6961 tctcgacctc ggaggccgct tcgccggccc ccttcaggtg gttgaagaag gcgttcaggg
     7021 cgacggccac gatcgagcag aggaggatgc cgctgtgaag gagcgggctg agggccttcg
     7081 gcatctgcgc gaagaacctg tccgcgacga gcgggatcat cccgaagccg atcgcgaggg
     7141 ccacgacgta gaggttgagg cggttcttcg cgaagtcgac gccgccgagg atgcggatcc
     7201 cggtggcgca gaccatgccg aacatgacga tccccgcgcc gccgaggacg aactgcggga
     7261 ccgaggcgac gacgaaggcg agcttcggga cgagtccgag ggcgaggagg aggacgccgc
     7321 cgaccgcggt gacccagcgg ctccgcactc ccgtcacgcc gacgaggccg acgttctggg
     7381 agaaggaggt gtagggaaag gtgttgaaga cgccgccgat gagggtgccg aggccgtcga
     7441 cgcggaggcc gtcggagagg tgcttctggt cgatctcctt gccgacgatg ttgccgacgg
     7501 cgaggaacat gccgagcgac tcgatcatga cgacgaccat gaccatgcac atcgtcaggc
     7561 aggcgacgaa gtcgaaggtc ggcaccccga actggaaggg gtagacgacc gcgaaccact
     7621 tctcgctcgc gagcccgccg aagtcgacct tgccgagggc gatcgcgatc accatcccga
     7681 ggacgaggcc gaggaggacg gcgatgttcg cgaagaagcc cttcacgaac ttcgtgacga
     7741 ggaggatgaa gacgaggacg atcccggcga cggcgaggtg gagcggcgcg ccgtaggccg
     7801 ggttcgggat catcgggccg gccatgtccg cggggttctt gatcatcggg ttgccgccgg
     7861 ccgcccagtt cacgccgacg cgcatgagcg agatgccgat gatggtgatg atcgacccgg
     7921 tcacgacggg cgggaagagc gggagcatcc ggctgatgag cggcgcgacg aggattccga
     7981 agaggcccgc cacgatgacg gcgccgtaga tcccgaggag gccgatctcc gggttcgtcg
     8041 ccatgacgac catcgggctc acggcggcga aggtgacccc catcatgacg gggaggcgga
     8101 tgccgaaccg cccgaggccg atcgactgga cgagggtcgc gatgccgcac gcgaagaggt
     8161 ccgcgttgat gagcatcgcc atctgctcct tggggagctt cagcgcgccg cccacgatga
     8221 gggggacggc gacggccccg gcgtacatga cgaggacgtg ctggaggccg aggagagtca
     8281 gccgcgcggg cggcaggacc tcgtcgaccg ggtggacgcc gttgttcgtg gccatgcgct
     8341 tccctcgctt cagacggcgg cgccgtcggg cttcgattcc gggaggggcg ggagggccgg
     8401 ctcggccgcc aggagcgcga cgaggcgcgc gcaggccgag aggtcgagga gcgcgccctc
     8461 ggccggctcc ccgacgaggc gcacgtcgac gacgcgccag gcgccggtct cgaggacgac
     8521 gcggagctcg tccgagcggt actccgcaag gccgccgtgg cgcgcgagac ggtgctgccg
     8581 gccgccgatg cgcgcgtcga tggcgacacc gtcgccggcg cgcgtcatcc ggtaggcgtc
     8641 ccccttcgcc tccgcgtagc cctcggcgtc cacccagaac gtcggcttct cctgccaggg
     8701 ggcgcccgcc gtcgggcaga aggggacgca attgccgcac tggttgcagg cgtcgccgag
     8761 gttgacgatc tggacgccct gctcgaccgc gaaggtcgtc gtccccgacg ggaccgggcg
     8821 tcccccctcg acgacgaagc gggggagctc gagcgcgacc ggggacgtct cgtaggcgag
     8881 gttcgcgcgg ttcgggcaga cggtgacgca gaggctgcag gcttcgtcgc agtcgaggca
     8941 gcgggccgcc tcggcgcgcg cggcatcggc cgggagggac tggaggacct cgtcgaagcc
     9001 gccacgccgg tccggcggga ggacgggcac ctcggccggt cgcgcgaggc gggccttctt
     9061 ctccatgagg gccgggagcg tcttcccctt cggcaggtgc ggctccgcgg cgggctcgac
     9121 gccgtggagg cgggcgatcg cgtcggccgc ggcgcgcccg tcggcgacgg ccttgatgat
     9181 ggaggcgggg ccgcggacga cgtcgccgcc ggcgaagagg ccggccgtcg aggtctcgcc
     9241 cgtcgcggcc tttgcgacga gcgtcccgtc cttcttcgcc tcgagggcgg cgccgccgag
     9301 gaactccaga gccgtctcct ggccgatggc ggtgacgatc gtgtcggccg gcaggacgac
     9361 ctcgctcccc tcgaccggaa tcggcctggg acggccggag gcgtcgggcg ggccgagccg
     9421 catcgcctgg caggcgaggc cgacggcctt cccgttctcg acgacgacgc ggaccggggc
     9481 gaggagggtc ttcaggccga ttccctcgac ctcgcagtcg tggacctcct cggggtcggc
     9541 gggcatctcg gcgcgagtcc gccggtagac gagggagacc tcgccgcccg ccccgacgag
     9601 ccgcttcgcg gagcgcgcgc cgtccatcgc ggagttgccg ccgccgacga cgaggacctt
     9661 cttcccgagg tcgcgcctcg ccccctcgcg gagcccgtcg aggaggacga gcgcgtcgag
     9721 gaccccctcg gcgtcctcgc cggggatccc gagcctcttc cccttctggg cgcccgcgcc
     9781 caggaagacg tagtcgaacc gggcgcggag gtcgtcgaga gagacgtcct ttccgaccgc
     9841 ggtgttcagg tggatcgcca cgccgaggga ccggaggcgc tcgaggtcga ccgcgagctt
     9901 cccgtcgtcg aggcggtacc tcggaacggc gccgccgacc attccgccgg cgtgcccctt
     9961 cgcctcgaag acctcggacg cgatgccggc ccgggcgagg ttcagcgcgg cggagaggcc
    10021 ggcgggcccg gcgccgacga tggcgaccct gacgcccttc gcctcgccgg cgacctcggg
    10081 cttcgaaacg ccgttctcga aggcgaagcg cttgatctcg cggatgccga gcggggcgtc
    10141 gtagaggttc ctcacgcagc gctcggtgca cgggtggtcg cagatgctgc cggtcacgcc
    10201 cgggagggcg ttcgaccggc ggatcacctc gatcgcctcc gtctcgcgcc cgttcgccac
    10261 gagccacagg tagtcgggga tgttctggtg cgccgggcag gcctccatgc agggcgccgc
    10321 gatgcagtcg aacggcccga gcgcgcgccc gcccttgaag gcgagggggc gctcgcggcg
    10381 cgcgaaacgt ccgcggcggc cgtccgcgac gacccgctcg acgtgcctcg cgaggttcca
    10441 ccgccgcccc ttgccgccgg aggtcttctc gacgaacgcg tcgaggctct ccgccccggc
    10501 ggcgtccatc gccgcgccga ggttgtcgag gtattgcgag aggcgggcgt agccgcccgg
    10561 cttcaggagg tcggtgcaga cggtgaccgg ggccatcccg tcggagacga ggtcggcgaa
    10621 gttccaggcg tcggcgccgc cgcagaagct gatcgggacg ctcccgtcga gctcgtcgtc
    10681 gacgagcttc gcgagcgaga gcgtgagcgg gtggagggcc cgccccgaca tgtacatcat
    10741 cttctcgctg gcggggaaga cggggcggtg gttgacgacc tcgagcgtgt tcgagagctt
    10801 cacgccgaac tcgcggcccg cggcgcgggc cgccgcggcg aggcgcttca ccatcgggag
    10861 ggcgtcctcg aagcgcgggt cgtggccgaa cgcggcgtcc gggacctcga cgccgtggcc
    10921 gaaagtctcg ttgagaaggc cgcggagccg ctcggggccg aggagcgtcg ggttcagctt
    10981 gacccaggtg tgaacgccca gctcccggag gaggtagagg gcgatcttct cgatctcggc
    11041 cggcgggcag ccgtgcatcg tcgagagggt gatgtggtcg gagatcgaag ccggaatcgc
    11101 gacgtcgcga acgccgggcc acgccttcgc gacggcctcg acggccgagg ggatcgaccc
    11161 ggaggcgtcg cgcatcgagg cgatgaaccg ctggacgcgc tcgctccgga tcccctcgag
    11221 gttgtagccg acgctcatgg cgaagagcgt cccgggagcc gggagggaga gcttcgcggc
    11281 gagcgcgtgg acgaggaccc aggccttcag gtactcgtcg aagctctgct cgagtctcag
    11341 ctcctgcgac cactcgcagt tgaaggtctc gtcggccgag tcgatgcagg ggcggctgat
    11401 ctcgagctcg tcgaggacct ggacggtctt cagctcgacg aagcgcgcgc cgcagagcca
    11461 ggcagcgacg atgttctggg cgagctggct gtgcggcccg gccgcgacgc cgaccggcgc
    11521 cgcgagctcg cggccgagga gacgggagga gaagcgcggc tcgggccgcg cgaaagagcc
    11581 cttcgggacc ccgaggaccg tctcgcgcgc gtcgagctcg ccgaagaccc atcgcgcgag
    11641 aagctcgagg ggaaggggac ggaacgcttt ttccacgatc gacctcctcg ggcggggggc
    11701 tcggcggagg cccggggcgg gcggaagccg ccccgggccg gaggagctac ttcgtggcga
    11761 cgaagacgct ggggtagcgc gcgaggaacg cggagcagtg gatcagctcg cgcgtgtcga
    11821 ggcactcgcc gatcttgtgg gtgttctgct cgatgccggt gccgaagccg agcatcgcgg
    11881 ggtacttgta ggggccgctc tcgatccagc ccttt
//
LOCUS       DAOTEZ010000081        11878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3783_length_11878_cov_6.183118, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000081 DAOTEZ010000000
VERSION     DAOTEZ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11878
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3783_length_11878_cov_6.183118"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="P5164_06180"
     CDS             complement(<1..555)
                     /locus_tag="P5164_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43518.1"
                     /translation="MTGSRGSLTARLIARDLHHYRWLILGTAIAGAASLVLTRFGDGD
                     GISTGPNVGFLLLLTTIIVLGVSLPMLVLLEHRTKAHLFVLSLPVSPAQYSFAKVASA
                     LVAFLLPWTAITGGVVGLTVLSPAPDGFLPFFVATMTFLLVNFCLLTALAVITRSEPA
                     AIAGILLPNFSVRRMPAIAAGSDRV"
     gene            complement(552..1460)
                     /locus_tag="P5164_06185"
     CDS             complement(552..1460)
                     /locus_tag="P5164_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY43519.1"
                     /translation="MTTTATPAIALRGVSKRYRFFALDDVSFRLEPGQILGFVGPNGA
                     GKSTTIRILMGLVRPDAGDVEVLGCRMPEEQVRAKWDIGFVSEEMRLFPGSALGGHLA
                     FVRSLFPGAWDDRYAARLLRRFHLHPEQRVRELSRGEQVKAALLLALARRPRLLLLDE
                     PTAGLDPVARQEVIGELMEVLRDEDRSILLSSHNTLDVEQVCDQIVFIDRGRIVDSGD
                     KEAFLERWRRLSLELPATIALPEVPGVVDVARSARTAVVTVNAFDEALPAAYQRVGAV
                     VRDVQRMTLEEIFVATVMNRRKERGE"
     gene            complement(1457..1846)
                     /locus_tag="P5164_06190"
     CDS             complement(1457..1846)
                     /locus_tag="P5164_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRY43520.1"
                     /translation="MHAELTISQADPRPMYQQIVDQVCRRIAVGDWEPGQEIPSIRAL
                     AASTRVSVITVKRAYLELEHQGVLTTRQGRGTFVSENVRLQQDLREQELDGHLARAVD
                     VGRELGLGTRELEQRLRKVIRQREEDR"
     gene            2002..2754
                     /locus_tag="P5164_06195"
     CDS             2002..2754
                     /locus_tag="P5164_06195"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HRY43521.1"
                     /translation="MNLILLFPEELGADGTARITGRRARHVHDVHRARVGDELVVGLR
                     DGATGRGTVLAVAADEVRLAVALVDVPPPPAGIDLLLAMPRPKVLRRVLQSAASLGAK
                     RLVLVNAARVEKSYFDTPFLAPEEIERNLVLGLEQARDTVLPEVLVRPRFRPFVEDEL
                     DTLWPREVFGRLLAHPADAPGLGAPDAPRVGPAAVVAIGPEGGWVPFETGLLEARGFA
                     RFGLGPRVLRVEAAVAFAFGQLLLARAFGTGR"
     gene            2800..4842
                     /locus_tag="P5164_06200"
     CDS             2800..4842
                     /locus_tag="P5164_06200"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HRY43522.1"
                     /translation="MRLGYGLALGLLLAVPAAAQDGPLPVPAAAPPAPSPGAPYRDAV
                     LSGRTANAAGLSFTVGAARYELATGSLWEVTTKDGTPVGAFFLGEGTLAFSAGDPQAA
                     RLAARNAKHVGGAKEVDGELRAAFSRATFLFSAALRPAWTYAAGEETPVRRFAAHVER
                     FARDRTPQVASRLAIAEAAKGAFFAATLEADPDLRHVLDPVTDDEEVLRVVDRPAGLP
                     GGFPEMRFSRDLSRRPLGRTRRQAPRVDARLVAVDVDVREGPAPWGELKVTETFVAVR
                     PVSFLVLDFATETIHRNDLLETRLRALTDGEGRPLPYALGDGELVVALPKPLAPGARL
                     TLRLDYEAPYFERAGGDNLWELPIASGWYPQPLSFNASHHTFHAVVRARKPFLAFASG
                     DTVRRTEEDGWNVLETRLERPVPFATVLAGKYTTQESTEDGVTCRVASYGIPKEMSGK
                     MLLSVFHGVRKFYEWLLGPFPWKEFTIVEINDYGFGQAPPGMMRITKEAFQSSIFTDE
                     VSSLFSHGINQRVAHEIAHAWFGYVVADASPEHQWISEAFSEIASMYAIERLKGKAEG
                     KKLAGTWAGSARHSAKAAPIDLANGLAPKIASTWDSSTAIDRVELVYSKGAHLLQTLR
                     LELGDDLFFTVLRSFLRSFEKQRDVTTDDFVALLSFATKKDWKPWFERYYYGTEMP"
     gene            complement(4858..7293)
                     /locus_tag="P5164_06205"
     CDS             complement(4858..7293)
                     /locus_tag="P5164_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRY43523.1"
                     /translation="MVRRSLVPTLALFPLLAVVATAAAQENVAFRTPPEEIRKLVDAP
                     LPPSVLVDPAGARLVLLDLPGYKTLAEVAEPELRLAGLRLNPKSHNRARLNVTTGISV
                     KEIASGKNARVEGLPAAPRIQWTRFSPRGTYFSFVQCDPDGLSLWVVDLASAKASRVT
                     PASVSAVLDFPYQWLPDESGLLVHVRPSLEPFAAPAELPAGPVVKVASGRKAPARTWQ
                     DLLKSENDEKTFAHYATTEVRRFALDGTSAAILPPAIRRSVRPSPDGKWILATTIVPP
                     FSYRFPVYRFGYRVDVLDAAGKKAATLVEKPVQDAIPVAFDATEAGRRDFDWREDAPA
                     TVFFAEAQDGGDPANDVPFRDRLYLLDAPFDGTPRAFAKTRNRFGEVTWGAGGIAVLS
                     DFRWKDRNTKTYLAKADAGDAEPKVLFDLSSENLYALPGDFVTRVDGRGRPLLLASRD
                     GKRLFLSGEGYSPEGNRPFLDAYELATGKTTRLWRADGTTTYERIVRVLDAERGVLLT
                     RVEGSKLFPNYQVRNVKARIAPRSVTSFENPYESLQAVATRKLRYRRADGIDLAGDLH
                     LPPGYDPKRDGRLPLLLEAYPTEYKDRAAAGMVDSSPHAFVRPYWGSPVFWALRGYAV
                     LENAQFPIVGEGEKEPNDTFVEQLVANAKAAIDALEKEGIVDPRRCAVTGHSYGAFMT
                     ANLLAHSDLFAAGIARSGAYNRSLTPFGFQAEERTYWQARDVYSRMSPFDHAAALNEP
                     ILLIHGEADNNPGTFTLQSERLFEAIQGLGGRSRLVLLPFESHGYAARENILHMLWET
                     DTWLETWVKGKKD"
     gene            complement(7344..7766)
                     /locus_tag="P5164_06210"
     CDS             complement(7344..7766)
                     /locus_tag="P5164_06210"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRY43524.1"
                     /translation="MAVDIDIVYTGGLHCEATHRPSGSKLVTDAPLDNGGKGEAFSPT
                     DLCATSLGSCLVTTMALVATRHGIELAGTTVHVVKEMVADPRRRIASLPTTVTFPTSL
                     ATLPAEKKALLEATARACPVTQSLGPNVEVPMTFVWPG"
     gene            complement(7831..8295)
                     /locus_tag="P5164_06215"
     CDS             complement(7831..8295)
                     /locus_tag="P5164_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43525.1"
                     /translation="MSRPRPLPEKARWLVLVAGLTASIPLYAVVVFVLATTGAPGASA
                     PIGLRYAVYGAAFGAALLALVLAPRVGSDAATSPAYPEFLRRTLVSLAVAEVSAILGV
                     VLFVLGRNLGDFLVLAGLALAVILLGIVPRGLRWFRLHEEPGGGGAPPLGPG"
     gene            8462..9928
                     /locus_tag="P5164_06220"
     CDS             8462..9928
                     /locus_tag="P5164_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HRY43526.1"
                     /translation="MEIDRESCLAAFLGRDRRFAGRFYAGVTSTGVYCRPGCPAPLPR
                     PSNVRFYPCAAAAEEAGFRACRRCRPETAPGTPAWAGTSATVARALRQIESGLVDHAP
                     LGVLAARLGVGERHLRRLFAEHLGASPVAVAKTRRLHFARRLLDETPLRMSDVALAAG
                     FSSVRRFNEAMRAAFGAPPTELRRAAAGRTASPAGVTLRLPFRPPFDSAALVAFLGAR
                     AIPGVESVDGSAWRRTAELAGVAGIVEAEVDAARSSIVVRLVGLPPGAALDAARRAGR
                     LFDTDADPAAIAAHLGKDPLLAPLVELRPGLRVPGAWDPFETAVRTVLGQQVSVKAAR
                     TLAARLVSRFGRPLPPGVARGSLTHLFPRPVDLAEADVASIGLPSSRGETIRVLAREV
                     AAGRLDLGPAASLEEAERRLAEIPGVGPWTAQAIALRSLGEPDAFPGGDLGVRKALAV
                     RGVAPTTAAAEARAAAWRPWRAYALVHLWTDLASREDS"
     gene            9925..10434
                     /locus_tag="P5164_06225"
     CDS             9925..10434
                     /locus_tag="P5164_06225"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRY43527.1"
                     /translation="MTTTLETATIETPAGGFWLAARDGALVAAEFAERVPHRLSRLEA
                     RFGPLELRDAADPAGAASALRRFLSGDLDALEEVPLDAGGTPFQRDVWAALREIPPGT
                     TATYAELAARVGRPAAVRAVGGANGANPVAVFVPCHRVVGKDGLRGYAGGLARKAWLL
                     AHEETAART"
     gene            complement(10487..11020)
                     /locus_tag="P5164_06230"
     CDS             complement(10487..11020)
                     /locus_tag="P5164_06230"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRY43528.1"
                     /translation="MKSRIENRLDDLDRWKDAFLGSLADLGPERLAFRADGVGWNALD
                     VVQHLVLVEEGVLAYARKKLLAPPQPISIVDRGKLALLVGVLRSPIRFRAPVAQVVPS
                     ETLPLEASATRWDQSRRDLRTLLLGLPEERRAALFFRHPAAGGLDPAGTLVFLHEHAR
                     HHEAQVRRIGRAPGAPR"
     gene            complement(11073..>11878)
                     /locus_tag="P5164_06235"
     CDS             complement(11073..>11878)
                     /locus_tag="P5164_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HRY43529.1"
                     /translation="AEIISSYTREAGVRQLERELGKLGRKVARRIAAGEIEKAAVDAA
                     DVSPILGRKKIQPEKIVHEDQVGVATGMYYTPAGGDIMFIEAALMRGKGEVLLTGQLG
                     DVMKESARAAWTFARSHAALLGIDETSFERDLHIHVPAGAIPKDGPSAGVAMASALVS
                     ALSKRPCRRDVAMTGEVTLSGRVLPIGGVKEKLLGAVRAGIRTVVLPKENVPDLEDLP
                     QDVRDGLEIVPVQDLSEALAVSLRGRVSGGQLMFAEEPAGTGTGAPPTH"
BASE COUNT         1591 a         4314 c         4359 g         1614 t
ORIGIN      
        1 cacccggtcg gagccggcgg cgatcgccgg catcctcctc accgagaagt tcgggaggag
       61 gatgccggcg atcgccgccg gctccgaccg ggtgatgacg gcgagggccg tgaggaggca
      121 gaagttgacg agcaggaacg tcatggtcgc gacgaagaac gggaggaagc cgtccggcgc
      181 cggggagagc acggtcaggc cgacgacgcc gcccgtgatc gccgtccacg gcaggaggaa
      241 ggcgacgaga gcggacgcga ccttcgcgaa cgagtactgc gccggcgaca ccggcaggct
      301 gaggacgaag aggtgcgcct tcgtccggtg ctcgaggagc acgagcatcg gcagggagac
      361 gccgaggacg atgatcgtcg tgaggagcag caggaagccg acgttcggcc cggtcgagat
      421 gccgtcgccg tcgccgaagc gcgtcaggac gagcgacgcc gcgccggcga tcgcggtccc
      481 gagtatcagc cagcggtagt ggtggaggtc cctggcgatc agccgggcgg tgagagatcc
      541 tcgcgagccg gtcattcgcc gcgctccttt cgcctgttca tgacggtggc gacgaagatc
      601 tcctcgagcg tcatccgctg gacgtcccgc accaccgcgc cgacccgctg gtacgccgcc
      661 ggcagcgcct cgtcgaaggc gttgaccgtg acgaccgcgg tgcgcgcgct ccgcgcgacg
      721 tcgacgactc ccggcacctc cgggagcgcg atcgtcgccg gaagctctag gctcaggcgc
      781 ctccaacgct cgaggaacgc ctccttgtcg cccgagtcga cgatccgtcc acgatcgatg
      841 aagacgatct ggtcgcagac ctgctcgacg tcgagggtgt tgtgagacga gagcaggatc
      901 gaacggtcct cgtcccgcag gacctccatg agctcgccga tgacctcctg gcgcgcgacg
      961 gggtcgaggc cggccgtcgg ctcgtcgagc agcagcagac gcggccggcg cgccagggcg
     1021 aggagcaggg ccgccttgac ctgctcgccg cgcgacagct cccggacccg ctgctcgggg
     1081 tggaggtgga atcgccggag gaggcgcgcg gcgtagcggt cgtcccaggc gccggggaag
     1141 agggagcgca cgaatgccag gtgcccgccg agcgccgatc cggggaacag acgcatctcc
     1201 tcggagacga acccgatgtc ccatttcgcg cgcacctgct cctcgggcat gcggcagccg
     1261 aggacctcca cgtctccggc atcgggacgg acgagaccca tgaggatccg gatggtggtg
     1321 ctcttcccgg cgccgttggg gccgacgaag ccgaggatct gccccggctc gaggcggaag
     1381 gagacgtcgt cgagcgcgaa gaagcgatag cgcttcgaga cgccgcgcag ggcgatcgcc
     1441 ggggtcgcgg tcgtggtcat cggtcttctt ctctctggcg gatgactttg cgaaggcgct
     1501 gctcgagctc cctcgtcccg aggccgagct cgcgtccgac gtcgacggcc ctggcgaggt
     1561 gcccgtcgag ctcctgctcc cgcaggtcct gctggagccg gacgttctcg gagacgaacg
     1621 tcccgcggcc ctgacgggtc gtgaggaccc cctggtgctc gagctcgagg taggcgcgct
     1681 tgacggtgat cacgctcacg cgcgtagacg cggcgagcgc gcggatcgac gggatctcct
     1741 gccccggctc ccagtcgccg accgcgatgc gccggcagac ctggtcgacg atctgctggt
     1801 acatcggcct cgggtcggcc tgcgagatgg tgagctcagc gtgcatatcg atatgcacac
     1861 tatatacggc tttcgagaac ggtcaagcgc cgggcgcgtc gccccggccg gggaagccgg
     1921 ccgggaaggc cgccgcccgt ctcaggcgcg atctcgcgcc gataatcgac ctctgcttcg
     1981 tatgcgacgc gagacccgac cgtgaacctg atcctcctct tccccgaaga gctcggagcg
     2041 gacggcaccg cgcggatcac gggccgccgc gcccgccacg tccacgacgt ccaccgcgcc
     2101 cgcgtcggcg acgagctcgt cgtcggcctg cgcgacggcg ccaccgggag gggcaccgtc
     2161 ctcgccgtcg cggccgacga ggtgcggctc gcggtcgcgc tcgtcgacgt gccgccgccc
     2221 cccgccggga tcgacctcct cctcgcgatg ccgcgcccga aggtcctccg gcgcgtcctc
     2281 cagtccgccg cgtcgctcgg cgcgaagcgg ctcgtcctcg tgaacgcggc gcgcgtcgag
     2341 aagagctact tcgacacccc gttcctcgcg ccggaggaga tcgagcggaa cctcgtcctc
     2401 ggcctcgagc aggcgcgcga cacggtcctc cccgaggtgc tcgtccgtcc gcgcttccgg
     2461 ccgttcgtgg aggacgagct cgacacgctc tggccgaggg aggtcttcgg acggctcctc
     2521 gcgcatcctg cggacgcccc ggggctcggc gcgccggacg cgccccgggt cggcccggcc
     2581 gccgtcgtcg cgatcggccc cgagggcggc tgggtcccgt tcgagaccgg actcctcgaa
     2641 gcgcgcggat tcgcacggtt cgggctcggc ccgcgtgtcc tccgcgtcga ggcggcggtc
     2701 gccttcgcgt tcggacagct gctgctcgcc cgcgccttcg gcaccggccg ctgacgcgcc
     2761 ggtgcgccgg gtgtgcgatc ccggtacgat cgcggaacca tgcgactcgg ttacggtctc
     2821 gcgctcggac tcctcctcgc cgtcccggcg gccgcccagg acggcccgct tcccgttccg
     2881 gcggccgcgc cccccgcgcc ctcgcccggc gcgccgtacc gcgacgccgt cctctcgggc
     2941 cgcaccgcga acgcggccgg cctctcgttc accgtcggcg ccgcgcgcta cgagctcgcg
     3001 acggggagcc tctgggaggt gacgacgaag gacgggacgc ccgtcggcgc gttcttcctc
     3061 ggggagggga cgctcgcgtt ctccgcggga gacccgcagg ccgcccgcct cgccgcccgg
     3121 aacgcgaagc acgtcggcgg cgcgaaggag gtcgacgggg agctccgggc ggccttctcg
     3181 agagcgacgt tcctcttctc cgcggcgctc cgccccgcgt ggacgtacgc cgccggggag
     3241 gagacaccgg tccgccgctt cgcggcgcac gtcgagcggt tcgcgcgcga ccggacgccg
     3301 caggtcgctt cccggctcgc gatcgcggag gcggcgaagg gggcgttctt cgccgcgacg
     3361 ctcgaggcgg acccggacct ccgtcacgtc ttggaccccg tgaccgacga cgaggaggtg
     3421 ctccgcgtcg tcgaccggcc ggccggactc ccggggggat tcccggagat gcgcttctcc
     3481 cgggacctct cccgccggcc gctcgggcgg acgcgccggc aggcgccgcg ggtcgacgcc
     3541 cggctcgtcg ccgtggacgt cgacgtacgc gaggggccgg cgccgtgggg cgagctgaag
     3601 gtgaccgaga ccttcgtcgc cgttcgcccc gtctcgttcc tcgtcctcga tttcgcgacc
     3661 gagacgatcc atcggaacga cctcctcgag acgcgccttc gcgccctcac cgacggagag
     3721 ggccggccgc tcccctacgc gctcggagac ggcgagctcg tcgtggccct cccgaagccc
     3781 ctcgcacccg gcgcgaggct cacgcttcgc ctcgactacg aggcgcctta cttcgagcgg
     3841 gccggcggag acaacctctg ggagctcccg atcgcgagcg ggtggtaccc gcagccgctc
     3901 tccttcaacg cgtcgcacca cacgttccac gccgtcgtcc gcgcccgcaa gccgttcctc
     3961 gcgttcgcct ccggggacac ggtccggcgg accgaggagg acgggtggaa cgtcctcgag
     4021 acgcggctcg agaggcccgt cccgttcgcg accgtcctcg caggcaagta cacgacgcag
     4081 gagtcgaccg aggacggcgt cacctgccgc gtcgcgagct acggcatccc gaaggagatg
     4141 tcggggaaga tgctcctgag cgtcttccac ggggtccgca agttctacga gtggctcctc
     4201 ggaccgttcc cctggaagga gttcacgatc gtcgagatca acgactacgg gttcggccag
     4261 gcgcctcccg gcatgatgcg gatcacgaag gaggcgttcc agtcgagcat cttcacggac
     4321 gaggtctcgt ccctgttctc gcacgggatc aaccagcggg tggcgcacga gatcgcccac
     4381 gcctggttcg ggtacgtcgt cgccgacgcc tcccccgagc accagtggat ctcggaggcg
     4441 ttctccgaga tcgcctcgat gtacgcgatc gagaggctga agggcaaggc ggaggggaag
     4501 aagctcgcgg ggacgtgggc ggggtccgcg cggcactcgg cgaaggcggc cccgatcgac
     4561 ctcgcgaacg gcctggcgcc gaagatcgcc tcgacctggg acagctcgac ggcgatcgac
     4621 cgggtcgagc tcgtctactc gaagggggcg cacctcctcc agacgctccg gctcgagctc
     4681 ggggacgacc tcttcttcac ggtcctccgg tcgttccttc ggagcttcga gaagcagcgc
     4741 gacgtgacga ccgacgactt cgtcgccctc ctctcgttcg ccacgaagaa ggactggaag
     4801 ccctggttcg agaggtacta ctacggcacc gagatgccct gagggcccgg ccccgcctca
     4861 gtccttcttc cccttcaccc acgtctcgag ccacgtgtcc gtctcccaga gcatgtggag
     4921 gatgttctcc cgcgcggcgt agccgtggct ctcgaacggg aggaggacga gccgcgaccg
     4981 gccgccgagc ccctggatcg cctcgaagag ccgctcgctc tggagcgtga aggtcccggg
     5041 gttgttgtcg gcctcgccgt ggatcaggag gatcggctcg ttcagcgccg cggcgtggtc
     5101 gaacggcgac atgcgcgagt agacgtcgcg ggcctgccag tacgtccgct cctcggcctg
     5161 gaagccgaac ggcgtgagag accggttgta cgccccggag cgcgcgatcc ccgcggcgaa
     5221 gaggtccgag tgcgcgagca ggttcgccgt catgaacgcg ccgtaggagt gccccgtgac
     5281 ggcgcagcgc ctcgggtcga cgatcccctc cttctcgagc gcgtcgatcg cggccttcgc
     5341 gttcgccacg agctgctcca cgaacgtgtc gttcggctcc ttttccccct cgccgacgat
     5401 cggaaactgc gcgttctcga ggacggcata gccgcggagc gcccagaaga cgggcgagcc
     5461 ccagtagggc cggacgaacg cgtgcgggga ggagtcgacc atgccggccg cggccctgtc
     5521 cttgtactcg gtcgggtagg cctcgaggag gagcgggagg cgcccgtcgc gcttcgggtc
     5581 gtacccgggc gggaggtgga ggtctcccgc gaggtcgatg ccgtcggcgc gccgatagcg
     5641 gagcttccgc gtcgcgacgg cctggagcga ttcgtaaggg ttctcgaacg acgtgacgga
     5701 acgcggcgcg atccgcgcct tcacgttccg gacctggtag ttcgggaaga gcttcgagcc
     5761 ctccacgcgc gtcaggagga cgccccgctc cgcgtcgagg acccggacga tccgctcgta
     5821 cgtcgtcgtg ccgtccgcgc gccacagccg ggtcgtcttc ccggtcgcga gctcgtacgc
     5881 gtcgaggaac ggccggttcc cctcgggaga gtagccctcg ccggagagaa agagccgctt
     5941 cccgtcgcgg ctcgcgagga ggagcggccg gccccggccg tcgactctcg tgacgaagtc
     6001 gcccggcagg gcgtagaggt tctcggagga gaggtcgaag aggaccttcg gctccgcgtc
     6061 cccggcgtcg gccttcgcga ggtaggtctt cgtgttccgg tccttccagc ggaagtccga
     6121 gaggacggcg atccctcccg cgccccaggt gacctcgccg aaccggttgc gcgtcttcgc
     6181 gaacgcccgg ggcgtcccgt cgaacggcgc gtcgaggagg tagagccggt cgcgaaacgg
     6241 gacgtcgttc gccgggtcgc cgccgtcctg ggcctcggcg aagaagaccg tcgccggcgc
     6301 gtcctcgcgc cagtcgaaat cgcggcgtcc cgcctccgtc gcgtcgaacg cgaccgggat
     6361 cgcgtcctgc accggcttct cgacgagcgt cgcggccttc ttccccgccg cgtcgaggac
     6421 gtcgacgcgg tacccgaatc ggtagaccgg gaaacggtac gagaagggcg ggacgatcgt
     6481 cgtggccagg atccacttcc cgtcggggga gggccgcacc gaccggcgaa tcgcgggcgg
     6541 gaggatcgcg gcgctcgtcc cgtcgagcgc gaaccggcgg acctcggtcg tcgcgtagtg
     6601 cgcgaacgtc ttctcgtcgt tctcgctctt cagcaggtcc tgccaggtcc gggcgggcgc
     6661 cttccgcccc gaggcgacct tcacgaccgg gcccgcgggg agctcggcgg gagcggcgaa
     6721 cggctcgagc gacgggcgga cgtggaccag gagccccgat tcgtccggga gccactggta
     6781 cgggaagtcg aggacggccg agacgctcgc cggcgtgacg cgcgaagcct tcgcgctcgc
     6841 gaggtcgacg acccagaggg agaggccgtc ggggtcgcac tggacgaacg agaagtacgt
     6901 ccccctcggc gagaagcggg tccactggat ccgaggcgcg gccgggagcc cctcgacgcg
     6961 cgcgttcttc ccggacgcga tctccttcac ggagatcccc gtcgtgacgt tgaggcgggc
     7021 gcggttgtgg ctcttcgggt tgagccgcag cccggcgagc cggagctcgg gttccgcgac
     7081 ctccgcgagc gtcttgtagc cggggaggtc gaggaggacg aggcgcgccc ccgccgggtc
     7141 gacgaggacg gacggcggca gcggcgcgtc gacgagcttc cggatctcct cgggcggagt
     7201 gcggaaggcg acgttctcct gggcggcggc cgtcgcgacg acggcgagga gcgggaagag
     7261 agcgagggtc gggacgaggc ttcggcgcac catcccgcaa ggctacccca gccctgcccc
     7321 ggcgacggcg gcgtccgccc ggctcagccc ggccagacga acgtcatcgg cacctcgacg
     7381 ttcgggccga ggctctgcgt caccgggcag gcgcgggccg tcgcctcgag gagggctttc
     7441 ttctcggcgg ggagagtcgc gagggaagtc gggaacgtca cggtcgtcgg gagcgacgcg
     7501 atccgccggc gcgggtcggc gaccatctcc ttcacgacgt ggacggtcgt cccggcgagc
     7561 tcgatgccgt gccgcgtcgc gacgagcgcc atcgtcgtga cgaggcagga gccgagagag
     7621 gtcgcgcaga ggtcggtcgg cgagaacgcc tcgccctttc cgccgttgtc gagcggggcg
     7681 tcggtgacga gcttcgagcc ggagggacga tgggtcgcct cgcagtggag gcccccggtg
     7741 tagacgatgt cgatgtcgac ggccatgccg gagacgctac gcgacgccgg ccgtcccgtc
     7801 gagccccgcc gcgcgggctc cggggacggc tcagcccggg ccgagcggag gcgccccgcc
     7861 gccgcccggc tcctcgtgca gccggaacca gcggaggccg cgcgggacga tcccgaggag
     7921 gatgaccgcg agcgcgagcc cggcgaggac gaggaagtcg ccgaggttcc ggccgaggac
     7981 gaagaggacg acacccagga tcgcggagac ctccgcgacc gcgagcgaga cgagcgtccg
     8041 ccggaggaac tcggggtacg ccgggctcgt cgcggcgtcg gatccgacgc gcggcgcgag
     8101 gacgagcgcg aggagcgccg ctccgaacgc ggctccgtag accgcgtagc gaagtccgat
     8161 cggcgcggat gcgcccgggg cgcccgtcgt cgcgaggacg aatacgacga cagcgtagag
     8221 agggatcgac gcggtcaggc cggcgacgag gacgagccag cgggccttct cgggaagggg
     8281 tctcggtcgg gacatgctct cgctccggcc cgacgatacc cccgccgatg cccgtcgcgg
     8341 cctctcggac ccgagggacc gaaccgactc cggcacctgc ccgaccgagc gacccggctc
     8401 ccccggcagc catgtccgaa aaccgccaga cagacccggc tccctgcgcg acactccgcc
     8461 cgtggagatc gaccgggagt cctgcctcgc cgccttcctc ggacgcgacc gccgcttcgc
     8521 cggccgcttc tacgccggag tgacgtcgac cggcgtctac tgccggcccg gctgccccgc
     8581 cccgcttccg aggccctcga acgtccggtt ctacccgtgc gcggcggccg ccgaggaggc
     8641 cgggttccgg gcctgccgcc gctgccggcc cgagacggct cccgggacgc cggcctgggc
     8701 ggggacgtcg gccaccgtcg cccgggcgct ccgccagatc gagtccgggc tcgtcgacca
     8761 cgcgccgctc ggtgttctcg cggcccggct cggcgtcggc gagcggcacc tccggcgcct
     8821 gttcgcggaa catctcgggg cctcgcccgt cgcggtcgcg aagacgcgcc ggctccactt
     8881 cgcgaggcgc ctcctcgacg agacgccgct ccggatgtcg gacgtcgcgc tcgccgccgg
     8941 gttctcgagc gtgcgccggt tcaacgaggc gatgcgcgcg gcgttcggag ccccgccgac
     9001 cgagctccgg cgcgctgcgg ccggccggac cgcgtctccg gccggcgtga cgctgcggct
     9061 cccgtttcgc cctccgttcg attccgccgc gctcgtcgcg ttcctcgggg ctcgggcgat
     9121 ccccggcgtc gagtccgtcg acggaagcgc ctggcgccgc acggcggagc tcgcgggagt
     9181 cgccgggatc gtcgaggcgg aggtcgacgc ggcgcgctcc tcgatcgtcg ttcgcctcgt
     9241 cggcctgccg cccggcgcgg cgctcgacgc ggcgcgccgg gccggacgcc tcttcgacac
     9301 cgatgccgac ccggccgcga tcgcggcgca cctcgggaag gacccgctcc tcgcgccgct
     9361 cgtcgagctt cggccgggac tccgcgtccc cggcgcgtgg gacccgttcg agacggcggt
     9421 gaggaccgtc ctcggccagc aggtgtcggt gaaggccgcg aggacgctcg ccgcgcggct
     9481 cgtctcgagg ttcggccggc cgctccctcc gggcgtcgcg cgcggctctc tcacgcacct
     9541 cttcccgcgc ccggtcgacc tcgccgaggc cgacgtcgcg tcgatcgggc tcccgtcgag
     9601 ccggggcgag acgatccgcg tcctcgcgcg ggaggtcgcc gccggccgcc tcgacctcgg
     9661 cccggccgcg tcgctcgagg aggcggaacg gcgcctcgcg gagattcccg gcgtcggacc
     9721 ctggacggcg caggcgatcg cgctccgctc gctcggcgag cccgacgcgt tcccgggggg
     9781 agacctcggg gtccgcaagg cgctcgcggt acggggcgtg gccccgacga ccgccgcggc
     9841 cgaggcgcgg gccgcggcct ggcgcccctg gcgggcctac gcgctcgtcc acctctggac
     9901 cgacctcgca tcacgggagg actcatgacg accacgctcg agaccgcaac gatcgaaacg
     9961 cccgcgggag ggttctggct cgccgcccgg gacggcgcgc tcgtcgccgc cgagttcgcc
    10021 gaacgcgtgc cgcatcgcct ttcccggctc gaggcccgct tcggcccgct cgagctccgg
    10081 gacgcggccg accccgccgg cgcggcgagc gcgttgcggc ggtttctctc cggcgacctg
    10141 gacgcgctcg aggaagtccc cctcgacgcc ggcggcacgc cgttccagcg cgacgtctgg
    10201 gccgcgctcc gcgagattcc tcccggcacg acggcgacgt acgccgaact cgccgcgcgc
    10261 gtcggccggc cggcggccgt gagggccgtc ggcggggcga acggcgcgaa cccggtcgcg
    10321 gtcttcgtcc cgtgccaccg cgtcgtcggg aaggacggcc ttcgcggcta cgccggcggc
    10381 ctcgcgcgca aggcgtggct cctcgcgcac gaggagaccg cggcccggac ctgacgagac
    10441 gaggcgtccc gcgccgcgag gcccggccgt cgcgcgccgg cccgactcag cggggcgcgc
    10501 ctggggcgcg gccgatccgc cggacctggg cctcgtggtg ccgggcgtgc tcgtggagga
    10561 agacgagcgt cccggccggg tcgagtccgc cggcggcggg gtgccggaag aagagcgccg
    10621 cgcggcgctc ctcgggcagg ccgaggagaa gggtccggag gtcgcgccgg gactggtccc
    10681 agcgcgtggc gctcgcctcg agcggaaggg tctccgaggg gacgacctgc gccacgggcg
    10741 cccggaaccg gatcggcgat cgcaggaccc cgacgaggag cgcgagcttc ccccggtcga
    10801 cgatcgagat cggctgcggc ggcgcgagga gcttcttcct cgcgtaggcg aggacgccct
    10861 cctcgacgag gacgaggtgc tggacgacgt cgagcgcgtt ccagccgacg ccgtccgccc
    10921 ggaacgcgag gcgctcgggt ccgaggtccg cgagggagcc gaggaaggcg tccttccatc
    10981 ggtccaggtc gtcgaggcga ttctcgatcc ggctcttcac ggcgcggagt cttccacgaa
    11041 gaccccgcgc cgggaagctc tgcggagcgc ggtcagtgcg ttggcggcgc gccggtcccg
    11101 gtcccggccg gctcctcggc gaacatcagc tggccgcccg acacccggcc gcggagcgag
    11161 acggcgagcg cctcgctgag gtcctgcacc gggacaatct cgagcccgtc gcgaacgtcc
    11221 tgcgggaggt cctcgaggtc cgggacgttc tccttcggga ggacgaccgt ccggatcccg
    11281 gcgcggaccg cgccgaggag cttctccttc acgccgccga tcgggaggac ccgcccggag
    11341 agcgtcacct ccccggtcat cgcgacgtcc cgtcggcagg gccgcttcga gagggcggag
    11401 acgagggccg aggccatcgc gacgccggcc gagggcccgt ccttcgggat cgccccggcg
    11461 gggacgtgga tgtgcaggtc acgctcgaac gaggtctcgt cgatcccgag gagcgcggcg
    11521 tgcgagcggg cgaaggtcca ggcggcccgc gccgactcct tcatgacgtc gccgagctgg
    11581 cccgtcagga ggacctcccc cttgccgcgc atcagcgccg cctcgatgaa catgatgtcg
    11641 ccgcccgccg gcgtgtagta catccccgtg gcgacgccga cctggtcctc gtggacgatc
    11701 ttctcgggct ggatcttctt gcggccgagg atcggcgaca cgtcggccgc gtcgacggcg
    11761 gccttctcga tctctcctgc ggcgatccgg cgcgccacct tccgtccgag cttcccgagc
    11821 tcgcgctcga gctggcggac gcccgcctcg cgcgtgtagg acgagatgat ctccgcga
//
LOCUS       DAOTEZ010000082        11713 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3846_length_11713_cov_6.900841, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000082 DAOTEZ010000000
VERSION     DAOTEZ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11713
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3846_length_11713_cov_6.900841"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..619)
                     /locus_tag="P5164_06240"
     CDS             complement(23..619)
                     /locus_tag="P5164_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43530.1"
                     /translation="MRELRSAVNAAFWFALFLLGWTLLVPPPEGTGKGSRPRFIRMTI
                     EDRGPGSHVERVRINVPWFLFRGGLHAVSASQIEHEADLQFQDPVTIDLLREVWKELS
                     EKPEGTDVARVHEDDELTFRKEKGEIRLTIREDVGENEGPPREIVTIRFPARFMEAAV
                     SGDRDLDLRALFEEMRGAARGDVLEVTSDDAHVRVVID"
     gene            complement(668..1243)
                     /locus_tag="P5164_06245"
     CDS             complement(668..1243)
                     /locus_tag="P5164_06245"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRY43531.1"
                     /translation="MHFSKTRIVVLAVAAIATLALGLAYRDTLRKGPAKVTEPPAPAA
                     GRPGASPVSLRDGGTRDLSAPSGKLLVVHFWATWCAPCEEELPGLVAWWSEAKADPRI
                     ELLAVSVDEEWPAVEAFLAKRNAAGLPVALDPKKTAASAFGTEKFPETWFLSPRGEVL
                     FHQVGPLDWGAPESRAALRALAEQALGPAAT"
     gene            complement(1135..2157)
                     /locus_tag="P5164_06250"
     CDS             complement(1135..2157)
                     /locus_tag="P5164_06250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HRY43532.1"
                     /translation="MRRRPRGPDFPLLGERLRTLEGDWRGRRLDSDPLELPHRYATPA
                     DREVVAFLAASLAFGRVASIRSSAERLLDALGPSPAATLARDAWDAPQLDGFVHRWVD
                     SRSLRPFLRAVGATLRSDGSLGALFADGDDGGPDYVPALESFFARLRERSDVSPRAAP
                     RGLLFLLPSPGNGSACKRAHLFLRWVVRTEGFDLGLWRGESFSPSRLLLPMDTHVHRI
                     SCYLGLTARPTADLGAAREATAALARIDPEDPVRFDWALSRLGILAACVRDPRRSLCG
                     DCAVRPVCRAASPDGRPPVPSRRTGADAACTSRRPGSSSSPSRRLPRSPSASPIATPS
                     GRVRRR"
     gene            2230..3375
                     /gene="hflX"
                     /locus_tag="P5164_06255"
     CDS             2230..3375
                     /gene="hflX"
                     /locus_tag="P5164_06255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRY43533.1"
                     /translation="MGERGAAGRAAGSAAGRKTEKAILVGVFGRSLGRAEAEDHLDEL
                     ERLVDTAGGVAVARALQERESPDPATYVGKGKLKELAEAAEAMEAGWIVFDDELTPSQ
                     TRNLEKELSVQVLDRANVILSIFASRARTREAMTQVELARLQYLLPRLTGAQSGLAQQ
                     RGGGAFRAGGGEKKLELDKRKIRRRIATLKEDLEKIEKSRNVRRRHLRNVATVSLVGY
                     TNAGKTTLFNRLSSSKELAEDRLFATLDARHARLLGVGGRAIVVSDTVGFLRKLPHAL
                     VASFRSTLKEVEEADLLVHVVDASSPHADEQRRVAEEVLAEMGVPADRVLLAYNKTDC
                     VGAVAPAGEIAISAVSGAGLADLRQSIVARLLSLGVAVPLLGAPPPE"
     gene            3372..8678
                     /locus_tag="P5164_06260"
     CDS             3372..8678
                     /locus_tag="P5164_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRY43534.1"
                     /translation="MSRDAAALPPRFEARGALSEATSPRVVAALDRETGRRVVLKIAG
                     GPGGEETLALRKEFRLLAALDHPSIVRARDFGVTPDGRAWFSTDEVDGPDLAAFAREH
                     APLENLSALADVAHALAYVHSRGIVHGDVKPQNVRVLGERAVLLDFGLAFARGEEVAG
                     IRGTPAYLAPEVLRGGVPDRRADLYALGVTFYEALTGVLPTAGRDLGGVLRFHLEEDV
                     PVASHVAPGLPSRLDRILSQLMERDPAARTPSARALLDELGRDFGLVRGSTWDARPEL
                     LAPPFSGRAELLGRFAQALRHAAEGSGKAIVVLGPEGAGKSRLLAEWRVLAQSEGALV
                     VEARAVAEDRTPYRPVLDVLSALTRRGGSAGAPARAALARVARLAATDVEAPPISRLT
                     EESSRLALFDGVLSALEATRRAEAGERPLVVLVDDLHLADRATAALLSFLFKAAESRP
                     IVVAAAAEPAPPPEPGGGPNEALAPIDDLTLPPWTLGPLSDAETVAAAAGALARRDLP
                     EDLSRLVFQETQGWPGGVVALLEQLVERRVVTLRDGQLVVDAELYRRPGRASPAAGRA
                     DSRLAALPPERRELLAALSIVPADLTFELALRLSQVGLPERVEAAEPEAGALSDALGA
                     LARSGLLVQRELAGEAVWEFPLARTREQLGASLPDERRRALHDAAAAWFEGRLEARAD
                     LLPAAALHALRGSEPERAIRLGLAAGARAERLFAYDQAARSYEGVLEFLDLAGRTGEK
                     AAVRERLGDVHFRAGNWRRALAAYHFLLKELGARPDASEPAVRRQAALLTFKIGMIRL
                     RRDDVEAARALFDRAEGELGTVGTTEERARLLDASARARLERGDVDGAEAKALAALEL
                     AGPELPDDLRSLLLGTLGSVAFLRGDVALAEERLESALGAARRSERNDLVRRALSSLA
                     AVLARTGRWDESEAMERECLDEAERSRDLWGITASLANLAMLQCGRGDYAAARAGLER
                     ALEIHRRLGAPLGQAQASVGLGTCDEALGRWDDAEQSYRLALDAVGDAPGRVAVDARR
                     ALGNLLRKRGDLDRAERSLFETLDAARGTGEPPLVASVLLPVALLERDRERLDEARRH
                     LDQVLETLAESGAADGLGRALVESAELAFRAGDLARCEKEAAEASEIARRLGDRHTLA
                     RILSLEARAAHQAHRAGDVDRLFEESARALSEIGAVYDLGRCYYEWGVRTLEKARAAA
                     RLGTAARLFERIGALRELERTRGVLERIATKAAAETAGPASLGLGVIGLYEVSRIVNS
                     TRDLSAVLEDIVDLALRRLNAERGMILLGDPVTGRLSVRVARNLKTGREDEAEAISRS
                     VVERVVADGRSVSSADARVDPRFSGRESIHAHSIVSFLCVPLLVKERASGAIYVDHRN
                     APRLFSEGDRAFLEAFADLAAVAIENARLVEELLEARLRLSVENESLKANLGRGWNLD
                     ALVGSSEAAGRIKATLPRAAAGPVTVLIRGESGTGKNLVARILHALSPRAQGPFIQFN
                     CAALPETLAESELFGHEKGSFTGADRKKPGRFELAQGGTIFLDEIGKTSLGIQAKLLR
                     VVEDKEFERVGGTQTLKCDAKIVAATNLDLEAAIRANEFREDLFYRLNVVPIELPPLR
                     SRLEDLPALVEHFVTKLSRDLGADPRRVEPEVLELFARYPWPGNIRELEATLHRALVL
                     SPEERLTPEEFPWILDSPLLLAGRPLVSRAPAAGGPEPPVDPDYYEARMAGLERELIE
                     RALAEAGGKIRDASRRLGLARNTLKAKMAKYGLRGQEEGAAT"
     gene            8675..10573
                     /locus_tag="P5164_06265"
     CDS             8675..10573
                     /locus_tag="P5164_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43535.1"
                     /translation="MTGEAGGPPRDGGSRRLALGAALVVFLLAALARLSNLAEAFPDG
                     RALFPPFDDLYHARRVADTFARFPFVLESDPLRGVAPLFCHWPPAWDLGLAAFAVAAG
                     ARSVAEALDAAAFVPPLLGALTAAVAAALATRRWGLLPGFVAGGLLGLTPALVEAAQV
                     SRIDHHAAEPLLVLALLAATARAAEARGGAAPDGLLLGGAVAGALLVHPALLLVAGLS
                     AVVLLFAASSRAPLAGAAGFALASAAVLVHRATRSEGYPESQWFLGTPHAGLLLGAAA
                     ALGAAALLRRRGWGRATAGAAAGAAGAAIAAAVPGTLPAFLEGSKFFGGDPWIDRVAE
                     QQPLLAGGAAAFPAVLLFLGGGLLLLPVLIVRRRGATPASGVLLLFTVALTLLAFSRA
                     RFAVQAAALLAVAAGAVVARLGHRPPGRAAALLALAPAVWGGAGLVHRPAPVVPDLAR
                     PALRAAVALRGRPEPGRVLTPENWGFVFLVGAGRGVLVDPFGSMSDPRAYERALGLYL
                     ETREERFVEACRHLGVRFVAFESPLAGLPETARRLGLPGEAFLREAAGGGPPTVTRLA
                     QATVWWRAYYDGGRARPERGPWGAPLTRLEKVYEDPARTPEVGAYGGSMLQLWRVDPG
                     PGPDVDRR"
     gene            complement(10580..>11713)
                     /locus_tag="P5164_06270"
     CDS             complement(10580..>11713)
                     /locus_tag="P5164_06270"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRY43536.1"
                     /translation="NFTQPMAMRLDEVVSGVKADVAVKVFGPDSAVLERLGGEVRRVL
                     ETVRGAADLQVEVLSGAAQVEIDLDRARMARYGLNVSDVRDVVETAVGGKTATEVLDG
                     PKRFAVVVRLPDELRRSPEAIASILLTAPGGEKVPLGKVARVATAPGPEAISHEQGSR
                     RLVVQTNVRGRDIGSFVAEAQKAIRAKLSVPAGYHLEWGGQFENQARATKRLMVVVPL
                     SLAIIFALLWLTFRLTRQAALVILNVPFALVGGIAALWLRDLNLNLSASVGFIALFGV
                     AVLNGVVMITSVNRLREEGMGLRLAVLAGASTRLKPVMMTALVAALGFLPMALSHGAG
                     AEIGRPLASVVIGGITTSTLLTLIVLPTLYEMVEARVARRRAS"
BASE COUNT         1419 a         4385 c         4403 g         1506 t
ORIGIN      
        1 tcggccgccg tccgtcccgt cctcagtcga tcacgaccct cacgtgggcg tcgtcgctcg
       61 tcacctcgag gacgtcgccg cgggcggctc cgcgcatctc ctcgaagagg gctcggaggt
      121 cgaggtcgcg gtcgccggag acggcggctt ccatgaaccg ggcggggaag cggatcgtga
      181 cgatctcgcg cggggggcct tcgttctcgc cgacgtcctc gcggatcgtc agccggatct
      241 cccccttctc cttgcggaac gtgagctcgt cgtcctcgtg gacccgggcg acgtcagtcc
      301 cctcgggctt ctcggagagc tccttccaga cctcgcggag gaggtcgatc gtgaccgggt
      361 cctggaactg gaggtccgcc tcgtgctcga tctggctggc ggagacggcg tggaggccgc
      421 cgcggaagag gaaccagggg acgttgatcc ggacgcgctc gacatggctc ccggggcctc
      481 ggtcctcgat cgtcatccgg atgaagcggg gccggctccc ctttcccgtc ccctccggcg
      541 gcggcacgag aagggtccag ccgaggagaa agagcgcgaa ccagaaggcg gcgttgacgg
      601 cggacctgag ctccctcata tcggtctctc cgtcctcagt aacggacgag gtgcgaaaag
      661 gttcccgtca ggtcgcggcg gggccgaggg cctgctccgc gagggcgcgg agagcggcgc
      721 gggattccgg agcgccccag tcgagggggc cgacctggtg gaagaggacc tcgcccctgg
      781 gggagaggaa ccaggtctcg gggaacttct ccgtcccgaa ggcggaggcc gccgtcttct
      841 tcgggtcgag ggcgaccggg aggccggcgg cgttgcgctt cgcgaggaag gcctcgacgg
      901 cgggccactc ctcgtcgacc gacacggcga ggagctcgat ccgcgggtcg gccttcgcct
      961 cgctccacca ggcgacgagg ccggggagct cctcctcgca gggggcgcac caggtggccc
     1021 agaagtggac gacgaggagc ttccccgatg gagcggagag gtcgcgcgtg ccgccgtccc
     1081 ggagggagac ggggctcgct ccgggccgcc cggcggccgg agcgggcggc tcggtcacct
     1141 tcgccggacc cttccggagg gtgtcgcgat aggcgaggcc gagggcgagc gtggcaatcg
     1201 ccgcgacggc gaggacgacg atccgggtct tcgagaagtg catgcggcgt cggctcctgt
     1261 gcggcgggat ggtacagggg gacgcccgtc ggggcttgcg gcccggcaga cggggcggac
     1321 ggcgcagtcg ccgcagagcg accggcgcgg gtcgcggacg cacgcggcga ggatgccgag
     1381 gcgcgaaagg gcccagtcga agcggaccgg gtcttcgggg tcgatccggg cgagggccgc
     1441 ggtcgcctcg cgggcagccc cgaggtcggc cgtcgggcgg gccgtcaggc cgaggtagca
     1501 ggagatccgg tggacgtggg tgtccatcgg gaggaggaga cgggacgggg agaagctctc
     1561 tccgcgccag aggccgaggt cgaaaccctc tgtccggacg acccaccgga ggaagaggtg
     1621 cgccctcttg caggcgctcc cgttgccggg ggaggggagg aggaagagga gcccgcgcgg
     1681 agccgctcgc ggggagacgt cgctccgttc ccggagccgg gcgaagaagc tctcgagggc
     1741 cgggacgtag tccggcccgc cgtcgtcccc atcggcgaag agggcgccga gggagccatc
     1801 ggagcgaagt gtcgccccga cggcccggag gaaggggcga agggatcggg aatcgaccca
     1861 cctgtggacg aatccgtcga gttgaggagc gtcccaggcg tcccgcgcga gggtggcggc
     1921 gggggagggg ccgagggcgt cgaggagccg ctcggcggag ctgcggatcg aagcgacccg
     1981 gccgaaggcg agcgaggcgg cgaggaaggc gacgacctcg cggtcggccg gtgtcgcgta
     2041 gcggtgcggg agctcgagcg gatcggagtc gagccggcgg ccgcgccaat ccccctcgag
     2101 ggtgcgcagg cgttccccga ggagcgggaa atccgggccg cgcggccgac gcctcatctc
     2161 cgcgatgcta tccggcccgg ggtctcattc ggacccgccc gtcgaggttg gctggtagca
     2221 tcaaaggtcg tgggcgaaag aggagcggcc ggacgcgcag cgggctccgc tgccggccgg
     2281 aagaccgaga aggcgatcct cgtcggagtg ttcggccgat cgctcggccg ggccgaggcg
     2341 gaggaccacc tcgacgagct ggagcggctc gtcgacaccg ccggcggcgt cgccgtcgcc
     2401 cgcgccctcc aggagcgcga gagccccgac ccggcgacct acgtcgggaa ggggaagctg
     2461 aaggagctcg cggaggcggc cgaggcgatg gaggccggct ggatcgtctt cgacgacgag
     2521 ctgaccccct cgcagacacg gaacctcgag aaggagctct cggtccaggt cctcgaccgg
     2581 gccaacgtca tcctctccat cttcgcctcc cgcgcgcgga cccgcgaggc gatgacgcag
     2641 gtcgagctgg cccgtctcca gtatctcctc ccgcgcctga ccggcgctca gtcgggtctc
     2701 gcgcagcagc gcggcggcgg cgcgttccgc gcggggggcg gcgagaagaa gctggagctc
     2761 gacaagcgga agatccgccg ccggatcgcg acgctgaagg aagacctcga gaagatcgag
     2821 aagagccgga acgtccgccg ccgccacctg cgcaacgtcg cgacggtctc cctcgtcggc
     2881 tacacgaacg ccggcaagac gacgctcttc aaccggctct cctcctcgaa ggagctcgcc
     2941 gaggaccgcc tcttcgcgac gctcgacgcg cgccacgcgc gcctcctcgg cgtcggcggg
     3001 cgggcgatcg tcgtgtcgga cacggtcggc ttcctccgga agctcccgca cgccctcgtc
     3061 gcctcgttcc gctcgacgct gaaggaggtc gaggaggccg acctcctcgt ccacgtcgtc
     3121 gacgcctcct cgccgcacgc cgacgagcag cggcgggtcg ccgaggaggt cctcgccgag
     3181 atgggcgtcc cggccgaccg ggtcctcctc gcgtacaaca agacggactg tgtcggggcg
     3241 gtggccccgg cgggagagat cgcgatctcg gccgtgagcg gcgcgggcct cgcggacctg
     3301 cgccagtcga tcgtcgcgcg gctcctctcg ctcggcgtgg cggtccctct gctcggagcg
     3361 ccgccgccgg aatgagccgg gacgcggccg cgctcccgcc gcgcttcgag gcgcgaggcg
     3421 ccctctcgga ggcgacgtcg ccccgtgtcg tcgccgcgct cgacagggag acgggccgcc
     3481 gcgtcgtcct gaagatcgcg ggcgggccgg gaggcgagga gacgctcgcg ctccggaagg
     3541 agttccggct cctcgccgcg ctcgaccacc cgtcgatcgt gagggcgcgg gacttcggcg
     3601 tgacgcccga cgggcgggcc tggttctcga ccgacgaggt cgacggcccc gacctcgcgg
     3661 cattcgcgcg cgagcacgcc cccctcgaga acctctcggc cctcgccgac gtggcccacg
     3721 ccctcgccta cgtccactcc cgcggcatcg tccacggcga cgtgaagccc cagaacgtgc
     3781 gggtgctcgg cgagcgcgcc gtcctcctcg acttcggcct cgcgttcgcg cgcggggagg
     3841 aggtggcggg gatccgcggg acgcccgcgt acctcgcgcc ggaagtcctc cgcggaggcg
     3901 ttcccgaccg gcgtgccgac ctctacgccc tcggcgtcac gttctacgag gcgctcacgg
     3961 gagtcctccc gacggcgggg cgcgacctgg gaggggtcct ccgcttccac ctcgaggagg
     4021 acgtgccggt cgcctcgcac gtggcgccgg gcctgccgag ccggctcgac cggatcctct
     4081 ctcagctgat ggagcgcgac ccggcggcgc ggacgccgtc ggcccgcgcc ctcctcgacg
     4141 agctgggacg cgacttcggc ctcgtccgcg gctccacgtg ggacgcccgc cccgagctcc
     4201 tggcgcctcc gttcagcggg cgggccgagc tcctcggccg cttcgcgcag gcgctgcgcc
     4261 acgcggcgga aggctccgga aaggcgatcg tcgtccttgg cccggaaggg gcggggaagt
     4321 cgcgcctcct cgccgagtgg cgggtcctcg cgcagtccga aggggcgctc gtggtcgagg
     4381 cgcgggccgt cgccgaggac cggacgccgt accgtcccgt cctcgacgtc ctctccgccc
     4441 tcactcgccg cggaggctcg gccggggcgc cggcgcgcgc cgcgctcgcc cgcgtcgccc
     4501 ggctcgcggc gaccgacgtg gaggcgccgc ccatctcgcg gctgaccgag gagtcgagcc
     4561 gactcgcgct cttcgacgga gtcctctcgg cgctcgaggc gacgcggcgc gcggaggccg
     4621 gcgaacgtcc gctcgtcgtc ctcgtcgacg acctccacct cgccgatcgc gcgaccgcgg
     4681 cgctcctgtc gttcctgttc aaggccgcag agtcgcgccc gatcgtcgtc gctgcggccg
     4741 ccgagcccgc gccgccgccc gagccggggg gcgggccgaa cgaggcgctc gccccgatcg
     4801 acgacctcac gctccccccg tggacgctcg ggccgctctc ggacgccgag acggtcgccg
     4861 ccgcggccgg ggcgctcgcg cggagggacc tccccgagga cctttcccgg ctcgtcttcc
     4921 aggagacgca gggctggccc ggaggagtcg tcgcgctcct cgagcagctc gtcgagcggc
     4981 gggtcgtcac gctgcgcgac ggccagctcg tcgtcgacgc cgagctctac cgccgcccgg
     5041 ggcgcgccag cccggccgcg gggcgggccg actcgcgcct ggcggcgctg ccgccggaga
     5101 ggcgcgagct cctcgcggcg ctctcgatcg tccccgccga cctgacgttc gagctcgccc
     5161 tccgcctctc gcaggtcggg ctccccgagc gcgtcgaggc ggctgaaccc gaggcgggcg
     5221 ccctctccga tgcgctcggg gccctcgcgc gttccgggct cctggtgcag cgggagctcg
     5281 cgggggaggc ggtctgggag ttcccgctcg cacggacgcg cgagcagctc ggggcctcgc
     5341 ttcccgacga acggcgccgc gcgctccacg acgccgcggc ggcctggttc gaggggaggc
     5401 tcgaggcgcg cgccgacctc ctcccggccg ccgcgctcca cgcgctccgc gggagcgagc
     5461 cggagcgggc gatccgcctc ggcctggccg ccggagcccg cgcggagcgg ctcttcgcct
     5521 acgaccaggc ggcgcggtcg tacgaggggg tgctggagtt cctcgacctg gcggggcgga
     5581 ccggggagaa ggcggcggtc cgcgagcggc tcggcgacgt ccacttccgc gccggcaact
     5641 ggaggcgggc gctcgcggca taccacttcc tcctgaagga gctgggcgcc cgtcccgacg
     5701 cgagcgagcc ggccgtccgc cgccaggccg ccctcctcac gttcaagatc gggatgatcc
     5761 ggctccgccg ggacgacgtg gaggcggcgc gtgcgctctt cgaccgggcg gagggggagc
     5821 tcggaaccgt cggcacgacc gaggagcggg cccggctcct cgacgcctcg gcccgggcgc
     5881 ggctcgaaag gggcgacgtg gacggggccg aggccaaggc gctcgccgcc ctggagctcg
     5941 cgggaccgga gctgcccgac gacctccgct cgctcctcct cgggacgctc ggctccgtcg
     6001 ccttcctgcg cggcgacgtg gcgctcgccg aggagaggct ggagtcggcc ctcggggcgg
     6061 cccggcggag cgagcgcaac gacctcgtgc ggagggcgct ctcgtctctc gcggcggtcc
     6121 tcgcgcggac gggacggtgg gacgagagcg aggcgatgga gcgagagtgc ctcgacgagg
     6181 cggaacgctc gcgcgacctc tgggggatca ccgcctcgct cgcgaacctc gcgatgctcc
     6241 agtgcgggcg cggcgactac gcggcggcgc gggccggcct cgagcgtgcg ctcgagatcc
     6301 accgccgcct gggcgccccg ctcgggcagg cgcaggcctc ggtcgggctc gggacgtgcg
     6361 acgaggcgct cggccgctgg gacgacgccg agcagagcta ccgcctcgcg ctcgacgccg
     6421 tcggcgacgc cccgggacgg gtcgcggtcg atgcccgccg cgccctcggg aacctcctcc
     6481 gcaagagggg agacctcgac cgggccgagc gctccctctt cgagactctc gacgcggccc
     6541 gcgggacggg cgagccgccg ctcgtggcgt ccgtcctcct tccggtcgcg ctgctcgagc
     6601 gggaccggga gcgcctcgac gaggctcggc gtcacctcga ccaggtcctc gagacgctcg
     6661 ccgagtcggg ggccgcggac ggcctcggcc gggcgctcgt ggagtcggcc gagctcgcgt
     6721 tccgggcggg ggacctcgcc cgctgcgaga aggaggcggc cgaggcgtcg gagatcgcgc
     6781 gccgcctcgg cgaccgccac acgctcgcgc ggatcctctc gctcgaggcg cgcgccgccc
     6841 accaggcgca ccgcgcgggc gacgtcgacc gcctgttcga ggagtccgca cgggccctct
     6901 cggagatcgg cgccgtctac gacctcggcc gctgctacta cgagtggggc gtgcggacgc
     6961 tcgagaaggc gcgcgccgcg gcccgcctcg gcaccgcggc ccgcctcttc gagcggatcg
     7021 gcgccctgcg cgagctggag cggacgcgcg gcgtcctcga gcggatcgcg acgaaggcgg
     7081 ccgccgagac ggccggcccg gcgtccctcg gcctcggggt catcggcctt tacgaggtct
     7141 cgcgcatcgt caactcgacg cgcgacctgt cggccgtcct cgaggacatc gtcgacctcg
     7201 cgctccgccg cctgaacgcc gagcgcggga tgatcctcct cggcgacccg gtcaccggcc
     7261 gcctctccgt ccgcgtcgcg cggaacctga agaccggccg cgaggacgag gcggaggcga
     7321 tctcccgctc cgtggtcgag cgcgtcgtcg ccgacggccg ctccgtcagc tcggccgacg
     7381 cgcgggtcga cccgcgcttc tccggccgcg agtcgatcca cgcgcactcg atcgtctcct
     7441 tcctctgcgt gccgctcctc gtgaaggagc gggcgtccgg ggcgatctac gtcgaccacc
     7501 gcaacgcccc ccggctcttc tcggaagggg accgcgcctt cctcgaggcg ttcgccgacc
     7561 tggcggcggt cgccatcgag aacgcgcgcc tcgtcgagga gctcctcgag gcgcgcctcc
     7621 ggctctccgt cgagaacgag agcctgaagg cgaacctcgg gcgcggctgg aacctcgacg
     7681 cgctcgtcgg gagctcggag gcggcgggcc ggatcaaggc gaccctcccg cgcgccgcgg
     7741 ccgggccggt caccgtcctg atccgcggag agtcggggac cggcaagaac ctcgtcgcgc
     7801 ggatactcca cgccctctcg ccccgcgcgc aggggccgtt catccagttc aactgcgccg
     7861 cgctccccga gacgctcgcc gagtcggagc tcttcgggca cgagaagggc tcgttcacgg
     7921 gcgccgaccg gaagaagccc gggcgcttcg agctcgctca gggagggacg atcttcctcg
     7981 acgagatcgg gaagacgtcg ctcggcattc aggccaagct cctgcgcgtc gtcgaggaca
     8041 aggagttcga gagggtcgga gggacgcaga cgctcaagtg cgacgcgaag atcgtcgcgg
     8101 cgacgaacct ggacctcgag gcggcgatcc gggcgaacga gttccgggag gacctcttct
     8161 accggctgaa cgtcgtgccg atcgagctcc cgccgctccg gagccggctg gaggacctgc
     8221 cggcgctcgt cgagcacttc gtcacgaagc tctcgcgcga cctcggcgcc gacccgcgcc
     8281 gcgtcgagcc ggaggtcctc gagctcttcg cccgctaccc gtggccgggg aacatccgcg
     8341 agctggaggc gacgcttcac cgggccctcg tcctcagccc cgaagagcgg ctcacccccg
     8401 aggagttccc gtggatcctc gactccccac tcctcctcgc ggggcgcccc ctcgtctcgc
     8461 gggccccggc cgccggggga cccgagcctc cggtcgaccc cgactactac gaggcgcgga
     8521 tggccggcct cgagcgcgag ctgatcgagc gcgcgctcgc cgaggcgggc gggaagatcc
     8581 gcgacgcctc ccgccgcctc ggcctcgccc ggaacaccct caaggcgaag atggcgaagt
     8641 acggcctgcg gggacaggag gagggagccg cgacgtgacg ggggaggcgg gcggaccgcc
     8701 gcgggatggc ggctcgcgcc gcctcgctct cggcgcggcg ctcgtcgtct tcctcctcgc
     8761 cgcgctcgca cgcctctcga acctggcgga ggcattcccc gacgggcggg cccttttccc
     8821 cccgttcgac gacctctacc atgcgcggcg ggtcgccgac acgttcgcgc ggttcccttt
     8881 cgtcctcgag agcgacccgc tccgcggcgt cgcgcctctc ttctgccact ggccgccggc
     8941 ctgggacctc ggcctcgccg ccttcgccgt agcggccgga gcgcggagcg tcgcggaggc
     9001 gctcgacgcg gcggcgttcg tcccgccgct cctcggcgcc ctgacggccg ccgtcgcggc
     9061 ggcgctcgcg acccggcggt ggggcctcct tcccggcttc gtcgcgggcg gcctcctcgg
     9121 cctgacgccg gcgctcgtcg aggccgcgca ggtgagccgg atcgaccacc acgcggccga
     9181 gccgctcctc gtcctcgcgc tcctcgcggc gaccgcgagg gccgcggagg cgaggggagg
     9241 ggccgcgccc gacgggctcc tcctcggcgg cgccgtcgcg ggggcgctcc tcgtccaccc
     9301 ggcgctcctc ctcgtcgccg ggctctcggc cgtcgtcctc ctcttcgccg cctcttcccg
     9361 cgcgccgctc gcgggcgcgg ccggattcgc cctcgcttcg gccgcggtcc tcgtgcatcg
     9421 cgcgacccgg tccgagggct atcccgagag ccagtggttc ctcggcaccc cgcacgccgg
     9481 cctgctcctc ggcgccgcgg ccgcgctcgg cgcagcggcg ctcctccggc ggagaggatg
     9541 gggccgcgcg acagcgggtg ccgccgcggg cgcggcgggc gcggcgatcg ccgcggccgt
     9601 tcccgggacg cttcccgcct tcctcgaggg ctcgaagttc ttcggcggcg acccgtggat
     9661 cgaccgcgtg gccgagcagc aaccgctcct cgccggcggg gcggcggcgt ttcccgccgt
     9721 cctcctcttc ctcggggggg ggctgctcct tctccccgtc ctcatcgtcc ggcggcgggg
     9781 cgcgactccc gcgtcgggcg tcctgctcct cttcaccgtc gccctgacgc tcctcgcctt
     9841 ctcgcgggcg cgcttcgcgg tccaggcggc ggcgctcctg gcggtcgccg ccggggcggt
     9901 cgtggcgagg ctggggcacc ggccgccggg ccgggccgcg gccctcctcg cgctcgcccc
     9961 cgccgtctgg ggcggagcgg gcctcgtcca ccgcccggcg cccgtcgtgc cggacctggc
    10021 gcgtccggcc ctccgcgccg ccgtcgccct ccgcgggcgt cccgagcccg gacgcgtcct
    10081 gacgccggag aactggggct tcgtcttcct cgtcggcgcg gggcggggcg tcctcgtcga
    10141 cccgttcggg agcatgagcg acccgcgcgc ctacgagcgg gctctcggcc tctacctcga
    10201 gacgcgggaa gagcgcttcg tcgaggcgtg ccggcacctg ggcgtccgct tcgtcgcgtt
    10261 cgagagcccg ctcgcggggc tgccggagac ggcgcggcgc ctcggcctgc cgggggaggc
    10321 tttcctgcgg gaggcggcgg gaggcgggcc gccgaccgtc acgcggctcg cgcaggcgac
    10381 cgtctggtgg cgcgcctact acgacggcgg gcgcgcgcgc cccgagcgcg ggccgtgggg
    10441 cgcgccgctc acccggctcg agaaggtcta cgaggacccg gcgcggacgc ccgaggtcgg
    10501 cgcgtacggc ggctcgatgc tccagctctg gcgggtcgac cccgggccgg ggcccgatgt
    10561 tgaccgtcgc tgatccgcct cagctcgctc gtcgccgcgc gacgcgcgcc tcgaccatct
    10621 cgtagagcgt gggcaggacg atgagcgtca ggagcgtcga ggtcgtgatc ccgccgatga
    10681 cgacggacgc gagaggccgc ccgatctcgg cccccgcccc gtgcgagagc gccatcggca
    10741 ggaagccgag cgccgcgacg agggccgtca tcatgaccgg cttcagccgc gtcgaggcgc
    10801 ccgcgaggac ggcgaggcgc agccccatcc cctcctcgcg cagacggttc accgacgtga
    10861 tcatgacgac gccgttcagg acggcgactc cgaagagcgc gatgaagccg accgaggccg
    10921 agaggttcag gttcaggtcg cgcagccaga gcgcggcgat cccgccgacg agcgcgaagg
    10981 ggacgttcag gatgacgagc gccgcctggc gcgtcagccg gaacgtcagc cagaggagcg
    11041 cgaagatgat cgcgagcgag agcgggacga cgaccatgag ccgcttcgtg gcccgggcct
    11101 ggttctcgaa ctgcccgccc cactcgaggt ggtacccggc gggcaccgag agcttcgccc
    11161 ggatcgcctt ctgcgcctcg gcgacgaagc tcccgatgtc cctcccgcgg acgttcgtct
    11221 ggacgacgag gcgccgcgag ccctgctcgt ggctgatcgc ctcggggccg ggcgccgtcg
    11281 cgacgcgggc caccttcccg agcggcacct tctcgccgcc gggcgccgtc aggaggatcg
    11341 aggcgatcgc ctcggggctc cggcggagct cgtcggggag cctcacgacg acggcgaagc
    11401 gcttcggccc gtcgaggacc tccgtcgcgg tcttcccgcc gacggccgtc tcgacgacgt
    11461 ccctcacgtc ggagacgttc aggccgtagc gcgccatccg cgcccggtcg aggtcgatct
    11521 cgacctgcgc ggcgcccgag aggacctcga cctgcaggtc cgccgcgccc cggaccgtct
    11581 cgaggacgcg ccgcacctcg ccgccgagcc gctcgaggac cgcgctgtcg gggccgaaga
    11641 ccttgacggc gacgtcggcc ttcacgcccg agacgacctc gtcgagccgc atcgccatcg
    11701 gctgcgtgaa gtt
//
LOCUS       DAOTEZ010000083        11712 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3847_length_11712_cov_6.726259, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000083 DAOTEZ010000000
VERSION     DAOTEZ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11712
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3847_length_11712_cov_6.726259"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..1371)
                     /locus_tag="P5164_06275"
     CDS             complement(46..1371)
                     /locus_tag="P5164_06275"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY43537.1"
                     /translation="MSTGMLVALAVAAVAVIAAVVLFRSLSALKTASAEQSAQLEEKA
                     GRLEQEKERLAGQLASAIEERDALAEKSAELGTRLAAAEAELETTKAERDSLADKAKR
                     EQTTQRFFFGRKAREAFEVFRQRLAADGFPERLEELAGENPKTLTEEARKILKDVESW
                     AEQGGVEDASILHTLALVDYSRGDVERARLRLRKAARTSSDALLFENLGDLYRISGKL
                     RTALENYKIAAKTAPETSPVHRKLGLALYDAKDFGAAVKPLKVALEQHKEDPDLHLRT
                     ARALLESGDFQGTVEVAQTAGRKFPKDPLLPSCAILAYGRMKKFRDAQKTFEKAVEID
                     PKSADVHVAHGMALLDEGKLPEAAESFRTALECDASRPEAHYGLGLAANRDLAFDTAL
                     GHLKKSVGLKPDYAEAWYAMKTTYEGLKKFEAAVEALNKAIALNPRLSA"
     gene            1465..3639
                     /locus_tag="P5164_06280"
     CDS             1465..3639
                     /locus_tag="P5164_06280"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRY43538.1"
                     /translation="MPHLRMTSRPAAGLFLPAVLFAVLAAPAPAARSEEVKGRVVVLG
                     FDGADHRLVSQMIAEGKLPNLAKLAREGSFSPLRPTIPAQTPVSWSTFSTGLSPGRTL
                     IFDFLKRDPKTYRPEFAIVSEGRKSFLLGRGNRAGAAAIGALLAFLFVFLIGRLVLKR
                     WKPAALVALGLALPAAFFSYRFGGLLPESLQTVINHRQGTPFWEVAGKSGIKSVVLHV
                     PVTFPAVDYDEGRLLSGLGVPDLRGRIGTPSYYTSDPFFAPKNKNEFSVELIRLESNV
                     GTIRTEVFGPYNKLFPEPPVIKTPMTLTVLPDGGRLRIEPEGSDAVTIKPGEWSPWVT
                     FTFAFNPVVKLTGIGRFHLTRIAPEIELYLSPIHFNPVDLPPNVKITAPRGLAKSLAK
                     RFGLYKTAGWSIDTWSMSEETIDEPTFLDDVTHTVQQYRKMMDGLLSEKGVRLYVQVY
                     EFTDRVGHVMWRFLDPEHPMYDAEKAAKFGPSVERNYRQMDQIVGDAQKELGPDDLLL
                     VCSDHGFSSWRRSINYNTWLARNGYLGMKGGEEKLADLEQLFSQGEFWPNVDWSRTRA
                     YSMGLGEIYVNLKGREAQGTVEPGAEYDAVRSEIKAGLMALVDEATGRRPVERVYFRE
                     EAYPSFDANVIPDIFVTNSDGYRVGWQATLGVVTPDLYEENRQVWSGDHCSVDPDVVP
                     GILFSSRPLRTTPRPGMADVPATIYKALGLTPPEPLDGAPLF"
     gene            3879..4859
                     /locus_tag="P5164_06285"
     CDS             3879..4859
                     /locus_tag="P5164_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRY43539.1"
                     /translation="MRQQLEAAQANLELRTAERRILELRAAETERAAGQAQVERDHAR
                     RQSLALRDDLARRLDALYRMGRLGYLQTLATADSGEAFLRGLQLLSFLASRDARLLRE
                     YEGSLAELAVKERNLAALRAAIAGVAAETLAAEKALQRAHAEKASLLARLERAAEEQR
                     SNVAALEDKTARLEALLELLETRGRALPAGSASIRRYKGALDWPVRGKVVVPFGRIAN
                     PRFPRTFLRSSGWTIDAPTGTPVRAIFAGDVVYAQWLKGYGNLVVLDHGDGVFTLYGH
                     LMTGTVPRGTRVALGEVLGRVADPPEEEVAGVHFEIRESRTSVDPRGWLR"
     gene            4943..6262
                     /locus_tag="P5164_06290"
     CDS             4943..6262
                     /locus_tag="P5164_06290"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRY43540.1"
                     /translation="MTKSRLASFLGSLALLGTLFVVLPGLGADEKKDKGAKDGLYQPL
                     GLFTEVLSLVRGNYVEQVEVRPLMAGAFAGMTEAMDPFSEYVAPEKMAAFEAARAARE
                     KADHVESGLVLARRAGYPVVVTAVPGSPAAAAGIRTDDLIEKVGDVSARSLALWEVEA
                     ALSGKPGGKGTVLVVREEKPRRRTLEIVRASWTPSLPSAERISGELVVKVPAFGAGTA
                     DALAKLLEPLDRTKSIVLDLRGNAWGTWEEAARAAALLGPPGPIGELKGRKIETRSWS
                     AEAGRRVHDGGLVLLVDSGTGGPAELFAAALREGAARELGVDVAAAARRDEKTAEAFA
                     AEEPGAAAAAPDKSKLRVRVVGEPTFGMGLTQEVVRLASGGALRITVGKVRTAAGRSL
                     SPRGLSPDDRVFHVLPEDGEEGKAPDPFLERALKVVAELPAPRARAS"
     gene            6259..7176
                     /locus_tag="P5164_06295"
     CDS             6259..7176
                     /locus_tag="P5164_06295"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HRY43541.1"
                     /translation="MTAARRRGGCGRSLALLAGLALLFALAFLWLRYVRPPASPPPPT
                     PTPAPTVAAPTPVPTPTAAPRPTTVPPDFEPAAGTGRGAIAVVIDDVGNADGSLARLE
                     RLSGPLAIAVLPGAPQAREAAALAKRKGWDLLVHLPMAGSRGPYEPGTISTDDDDATI
                     VRRVEEALAAVPGAIGLNNHQGSVATADRRVVRAVLSVVRAKGLFFLDSRTSAASVAT
                     EEARALGLAMIPRDVFLDDARAEAAAIGGAPEALETAFARALSTAATKGHAVVIGHPH
                     AATVDFLALRFPALAKSGVLRVRVSELVD"
     gene            7277..7696
                     /locus_tag="P5164_06300"
     CDS             7277..7696
                     /locus_tag="P5164_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HRY43542.1"
                     /translation="MSLFEDVELNDAQAGAVGRAMLAVARADGAADPREVALIEELAP
                     VDTSAPDPTPAELAETLKEEKGRELLLRSVLLVALVDGDYSAAERAVVGRFAEALGVS
                     GSRLEELASGVKAFLMAPLLPLANTSSVLDVSRKLKV"
     gene            7780..10305
                     /locus_tag="P5164_06305"
     CDS             7780..10305
                     /locus_tag="P5164_06305"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HRY43543.1"
                     /translation="MRAHRLRFALAASFVVSLALPASAAPPPAEHLGLAVGSDRTLAD
                     WGQIVSYFRALDAASDRVEVTTIGTSTRGEEMILAAISSPENLANQRRLREVARRLAD
                     PRGLSPAEEKALLVEGKAVLLVTCGIHASEIGASQMAMEWAHALATAEDAETKRRLSE
                     VVLLLVPSLNPDGHRMEVDWYRKNLGTRWEGSRMPWLYHHYVGHDNNRDFAQLTQAET
                     KAISRVLYHDWFPQVFLDEHQMGTSGPRMFVPPYADPLDPDIDPLVIREVNMIGSLMS
                     MRLEQAGKSGVISAWAFDVSWPGGSKNTAWWKNVTGVLTEVASVRLATPIEVPPTELR
                     GGGKGLVEYGPQVNFPNPWRGGKWRLRDVMDYERIASDAIHEYCATHREDVLRNMLAK
                     ARASIAAAAPGEGYRISMTQRDPATARAVAALLVEHGVEVKADSAGDAWVPLAQPYGR
                     FVREMLEVQRFPEVKLVPGKEIVAPYDVTAWSLPLSMGVTVERGRLPESLRPLGAVGP
                     VPTLPSGTTAVALGPESPERWKVVAAARTGGAVSILSAADGALAAGTIVLDAAAAQAA
                     SRAAADLGLSLAPLAAAPAAARPFRAPRVGLYKPWIASMDEGWTRFLLERYGFAPLTL
                     DNAALRAGKLGTLFDVIVLPDVEKEVIADGKPKREEGAMRYFPDPRPEYAGGLGKEGA
                     AALRAFVEGGGTLVALGSSTEYLVEELGLPVRNALARAKPDEFLCPGGLVRLDVSPAH
                     PVTWGLPPSVPGFLDGPLAFQTTIPGAEMTREVLAAYPADGRDVLVAGWIRGEEKLAR
                     NAAAVALTLGKGKVVLLGFRPQHRAQTNATFPFLFNSLAWSVM"
     gene            complement(10277..11605)
                     /locus_tag="P5164_06310"
     CDS             complement(10277..11605)
                     /locus_tag="P5164_06310"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY43544.1"
                     /translation="MPGRKSEVRAWLAYDWANSAWAMTVAVAVLPVYFAGTVVPKGGV
                     ELFGIRVDATVLWGYVNSASTLAVFLTAPILGAIADRLGARRKLLTVFGLAGGLLTML
                     LAACGPGDVATTLWIYALSQIAFVSANVFYDSFLPHLAKDEELDRLSARGFAVGYVGG
                     SLHLVLALVLMATHARWGLSAEAAARIALGSAGLWWAGFGAITFLGLKEPPRTGGKPG
                     LLRAVREGVADTLATTRRVAKVKPVLLFLLAFVLYNDGVQTVIKMASLYGTEEVGVGM
                     PMMMGTLLGIQLLAMVGALLFARLAGAWGTKRALVLAVALWLVAVGWAYLLTTPAGFV
                     VLAALAGLVLGAVQALSRSLFAAMIPRESSAELFGFFSVFNKLAGVLGTFLFATVRQA
                     TGTSRLAILSVAVLFVAGLVLLLRVNEAEARARRDELRGDDATSPTRRGS"
BASE COUNT         1629 a         4278 c         4222 g         1583 t
ORIGIN      
        1 ccgcaggcgc cgcggggagg ctcgtggcga gcctccccga tccactcagg ccgacaggcg
       61 cgggttgagc gcgatcgcct tgttcagggc ctcgacggcc gcctcgaact tcttcagccc
      121 ctcgtaggtc gtcttcatcg cgtaccacgc ctccgcgtag tcgggcttca ggcccacgga
      181 cttcttgagg tgcccgagcg cggtatcgaa ggcgaggtcg cggttcgcgg cgaggccgag
      241 cccgtagtgc gcctcggggc gggaggcgtc gcactcgaga gcggtccgga acgactccgc
      301 ggcctccggg agcttcccct cgtcgaggag ggccatcccg tgggccacgt ggacgtcggc
      361 gctcttcggg tcgatctcga ccgccttctc gaacgtcttc tgcgcgtcgc ggaacttctt
      421 catccggccg tacgcgagga tggcgcacga ggggaggagc gggtccttcg ggaacttccg
      481 gccggcagtc tgcgcgacct cgacggtccc ctggaagtcg cccgactcga ggagcgcccg
      541 cgccgtccgc aggtggaggt cgggatcctc cttgtgctgc tcgagggcga ccttcagcgg
      601 cttgacggcg gcgccgaagt ccttcgcgtc gtacagggcg aggccgagct tccggtggac
      661 gggggaggtc tccggggcgg tcttcgcggc gatcttgtag ttctcgagcg ccgtccggag
      721 cttcccggag atccggtaga gatcgccgag gttctcgaag aggagggcgt cggacgaggt
      781 ccgggccgcc ttgcgcaggc ggagccgggc gcgctcgacg tcgccccggg agtagtcgac
      841 gagggcgagc gtgtggagga tcgaggcgtc ctcgacgccc ccctgctccg cccacgactc
      901 gacgtccttg aggatcttcc gggcctcctc ggtgagcgtc ttcgggttct cgccggccag
      961 ctcctccagc cgttccggga agccgtccgc ggccagacgc tggcggaaga cctcgaaggc
     1021 ctcccgcgcc ttccggccga agaagaagcg ctgggtcgtc tgctcccgct tcgccttgtc
     1081 ggcgagcgag tcgcgctcgg ccttcgtggt ctccagctcg gcctcggccg cggcgaggcg
     1141 cgtgccgagc tcggcggact tctccgcgag ggcgtcccgc tcctcgatgg ccgaggccag
     1201 ctgcccggcg agccgctcct tctcctgctc gagccggccg gctttctcct cgagctgagc
     1261 gctctgctcg gcggaggcgg tcttgagggc ggacagggag cggaagagga cgacggcggc
     1321 gatcacggca acggccgcga cggcgagggc gacgagcatt ccggtcgaca agggactcct
     1381 ccgcgcaacg ggaacggcgc gaccccgaat tctacctccg cccgagggcc gggggcgggc
     1441 gcgaccgcgt gtttagaatg ccgcgtgccg cacctccgga tgacctcccg acccgccgcc
     1501 ggcctcttcc tcccggccgt cctcttcgcc gtcctcgccg cgccggcccc ggctgcccgc
     1561 tcggaggagg tgaaaggacg ggtcgtcgtc ctggggttcg acggggccga ccacaggctc
     1621 gtctcccaga tgatcgccga ggggaagctg ccgaacctgg cgaagctggc ccgggagggg
     1681 agcttctccc ccctccggcc gacgattccg gcccagaccc cggtctcctg gtccacgttc
     1741 tcgaccggcc tctccccggg ccggacgctg atcttcgact tcctgaagcg cgatccgaag
     1801 acctaccggc ccgagttcgc gatcgtctcc gagggaagga agagcttcct cctcggccgc
     1861 gggaaccggg cgggcgcggc ggcgatcggg gcgctcctcg cgttcctctt cgtcttcctg
     1921 atcggccggc tcgtcctgaa acggtggaag ccggcggccc tggtcgcgct cggcctcgcg
     1981 ctccccgccg ccttcttctc gtaccggttc ggcggcctcc tccccgagtc gctccagacg
     2041 gtcatcaatc accgccaggg gacgccgttc tgggaggtgg ccgggaagag cgggatcaag
     2101 tccgtcgtcc tccacgtccc ggtgacgttc cccgcggtcg actacgacga ggggcgcctc
     2161 ctttcgggtc tcggagtccc ggacctgcgc gggcggatcg ggacaccgag ctactacacc
     2221 tccgacccgt tcttcgcccc gaagaacaag aacgagttct ccgtcgagct catccgcctc
     2281 gagtcgaacg tcgggacgat ccggacggag gttttcgggc cctacaacaa gctcttcccc
     2341 gagccgcccg tcatcaagac gccgatgacg ctcaccgtcc tccccgacgg gggccgcctg
     2401 cggatcgagc ccgaggggag cgacgcggtg acgatcaagc cgggggagtg gtccccctgg
     2461 gtcacgttca cgttcgcgtt caacccggtc gtcaagctca ccggcatcgg ccgtttccac
     2521 ctgacccgga tcgctcccga gatcgagctc tacctctcgc cgatccactt caacccggtc
     2581 gacctcccgc cgaacgtgaa gatcaccgcc ccgcgggggc tcgcgaagag cctggcgaag
     2641 cggttcggcc tctacaagac ggccggctgg tcgatcgaca cctggtcgat gagcgaggag
     2701 acgatcgacg agccgacgtt cctcgacgac gtgacccaca ccgtccagca gtaccggaag
     2761 atgatggacg ggctcctctc ggagaagggc gtgcggctct acgtccaggt ctacgagttc
     2821 accgaccggg tcgggcacgt gatgtggcgc ttcctcgacc cggagcaccc gatgtacgac
     2881 gcggagaagg cggcgaagtt cggcccgtcc gtcgagcgga actaccggca gatggaccag
     2941 atcgtcggcg acgcgcagaa ggagctcggc ccagacgacc tcctcctcgt ctgctccgac
     3001 cacggcttct cgagctggcg gcgctcgatc aactacaaca cctggctcgc ccggaacggg
     3061 tacctcggga tgaagggggg cgaggagaag ctggccgacc tcgagcagct cttcagccag
     3121 ggggagttct ggccgaacgt cgactggtcc cggacgaggg cctactcgat gggcctcggc
     3181 gagatctacg tcaacctgaa agggcgcgag gcgcagggga cggtcgagcc gggagccgag
     3241 tacgacgcgg tccggagcga gatcaaggcg gggctcatgg ccctggtcga cgaggcgacg
     3301 ggccgccgtc ccgtcgagag ggtctatttc cgcgaggagg cctatccctc cttcgacgcg
     3361 aacgtgatcc ccgacatctt cgtcacgaac agcgacggct atcgggtcgg atggcaggcg
     3421 accctcggcg tcgtgacgcc cgacctgtac gaggagaacc ggcaggtctg gtcgggcgat
     3481 cactgctccg tcgaccccga cgtcgtcccg gggatcctct tctcgagccg cccgctccgg
     3541 acgacgccgc gccccggcat ggccgacgtc ccggcgacga tctacaaggc gctcggcctg
     3601 acgccgcccg agccgctcga cggagcgccg ctcttctgac tcgagccggc gagccgcgcc
     3661 ccgggggcgg agcccgccgc gggcgtggct cgcggctcgg cctcctcctc ctgcccgggc
     3721 tgctcgccgc tcccgccctc cgcgggcagg agcgagctcc ggtccccgcg gcgcgggacg
     3781 cgtccggggc ggagcggcgc cgcgagctgg agtcgctccg ccgccggctc gcggggctga
     3841 aggcgcgcta cgaggagacc cggcagcggg ccgtcgacgt gcggcagcag ctggaggcgg
     3901 cccaggcgaa cctcgagctt cggaccgccg agcggcggat cctcgagctg cgcgccgcgg
     3961 agaccgaacg cgcggccgga caggcgcagg tcgagcggga ccacgcgcgc cggcagagcc
     4021 tcgccctccg ggacgacctc gcccggaggc tcgacgcgct ctaccggatg ggccgtctcg
     4081 gctatctcca gaccctggcc acggccgact ccggggaggc gttcctgaga gggctccagc
     4141 tcctctcgtt cctggcttcg cgggacgccc gcctgctgcg cgagtacgag gggtcgctcg
     4201 ccgagctcgc ggtcaaggaa cggaacctcg cggcgctgcg cgcggcgatc gccggcgtcg
     4261 cggccgagac gcttgcggcc gagaaggccc tccagcgggc gcacgccgag aaggcctccc
     4321 tcctcgcccg cctcgagagg gccgccgagg agcagcggtc gaacgtcgcc gcgctcgagg
     4381 acaagacggc ccgcctcgag gcgctcctcg agctcctcga gacgcgaggc cgggcgctcc
     4441 ccgccgggtc ggcctccatc cggaggtaca agggagcgct cgactggccc gtccgcggga
     4501 aggtcgtcgt ccccttcgga cggatcgcga acccccgctt cccgcgcacc ttccttcgct
     4561 cgagcggctg gacgatcgac gcgccgaccg gcacgccggt ccgcgccatc ttcgcgggcg
     4621 acgtcgtcta tgcccagtgg ctcaagggtt acggcaacct cgtcgtcctc gaccacggcg
     4681 acggtgtctt cacactctac ggccacctga tgacggggac ggttccccgg gggacccggg
     4741 tcgccctcgg cgaggtcctc gggcgggtcg cggacccgcc cgaggaggag gtcgccggcg
     4801 tccatttcga gatccgagag agtcgcacct cagtcgaccc gcggggctgg ctccggtagc
     4861 tcgccctccg gccggcacgg ccgcgcctgg gctgcgcggg cggcttcgga accgcaggaa
     4921 cgcgatcccg tatcatcgag tcgtgacgaa gtcccgtctc gcctcgttcc tcggctcgct
     4981 cgccctcctc ggcacgctct tcgtcgtcct cccgggcctc ggggccgacg agaagaagga
     5041 caagggggcg aaagacggcc tttatcagcc gctcggcctc ttcacggagg tcctttcgct
     5101 cgtgcggggc aactacgtcg agcaggtcga ggtccggccg ctcatggcgg gcgccttcgc
     5161 ggggatgacc gaggcgatgg acccgttctc ggagtacgtg gctccggaaa agatggccgc
     5221 cttcgaggcg gcgcgcgcgg cccgggagaa agccgaccac gtcgagtcgg gcctcgtcct
     5281 ggcgcgtcga gccggctacc cggtcgtcgt caccgccgtt cccggaagcc cggccgcggc
     5341 ggccggtatc cggacggacg acctgatcga gaaggtgggg gacgtctcgg ctcggagcct
     5401 cgccctctgg gaggtcgagg ccgcgctctc cggcaagccg ggggggaagg ggaccgtcct
     5461 cgtcgtccgg gaggagaagc cccggcggcg gacgctcgag atcgtgcgcg cctcctggac
     5521 cccgagcctg ccgtcggccg aacggatctc cggcgagctc gtcgtgaagg tgccggcctt
     5581 cggagcgggg acggccgacg ccctcgcgaa gctcctcgag ccgctcgacc ggacgaagtc
     5641 gatcgttctc gacctgcgcg ggaacgcctg ggggacctgg gaggaggcgg cacgcgccgc
     5701 cgcgctcctc ggcccgcccg ggccgatcgg cgagctgaag ggacggaaga tcgagacccg
     5761 cagctggtcc gcggaggcgg gccggcgcgt ccacgacgga gggctcgtcc tcctcgtcga
     5821 cagcggcacg ggcggaccgg ccgagctctt cgccgccgcg ctccgcgaag gagcggcccg
     5881 ggagctcgga gtggacgtcg cggccgcggc acgccgggac gagaagaccg ccgaggcctt
     5941 cgcggcggag gagccgggag cggcggcggc tgctccggac aagtcgaagc tccgggtccg
     6001 ggtcgtcggg gagccgacgt tcggcatggg actgacccag gaggtcgtcc gccttgcgag
     6061 cggaggcgcg ctccggatca cggtcggcaa ggtccggacg gcggccggcc ggtcgctctc
     6121 gccgcggggc ctctcccccg acgaccgcgt cttccacgtc ctcccggagg acggggagga
     6181 ggggaaagcg cccgacccgt tcctcgagcg ggcgctgaag gtcgtcgccg agctgccggc
     6241 cccccgcgca cgcgcctcgt gacggccgcg cggcggcggg gcggctgcgg gcggtcgctg
     6301 gctctcctcg cgggcctcgc gctcctcttc gccctcgcgt tcctctggct ccgctacgtg
     6361 cggccgccgg cctccccgcc accgccgacc ccgacgccgg cgccgacggt cgccgccccg
     6421 acccccgtcc cgactccgac ggcggcgcct cggccgacga ccgtccctcc cgacttcgag
     6481 ccggcggccg gcacgggacg cggggcgatc gccgtcgtca tcgacgacgt cgggaacgcc
     6541 gacggctccc tcgcccgcct cgagcggctt tccgggccgc tcgcgatcgc cgtcctcccc
     6601 ggcgcgcccc aggcgcggga ggccgcggcc ctcgcgaaac ggaaggggtg ggacctcctc
     6661 gtccaccttc cgatggcggg ctcgcgcggc ccctacgagc cggggacgat ctccaccgac
     6721 gacgacgacg ccacgatcgt ccggcgcgtg gaggaggcgc tcgccgccgt cccgggcgcg
     6781 atcggcctga acaaccacca gggctcggtc gcgacggcgg accgcagggt cgtccgggcc
     6841 gtcctctccg tcgtccgggc gaaggggctc ttcttcctcg actcgaggac ctcggcggcg
     6901 agcgtcgcga cggaggaggc gagggcgctc gggctcgcga tgatcccgcg cgacgtcttc
     6961 ctggacgacg cgcgggccga ggcggcggcg atcggcgggg cccccgaggc gctcgagacg
     7021 gcgtttgctc gggccctctc gacggcggcg acgaaggggc acgcggtcgt catcggccat
     7081 ccccacgcgg cgacggtgga ctttctcgcg ctccgcttcc ccgccctcgc gaagagcggc
     7141 gtcctccggg tccgggtctc cgagctcgtc gactgagcgg cgcgcccggg ggcgccccgg
     7201 cggctccttc gggccgcgtc gcgggcggta tcatcgccgc cgccactcgg cagaacgaaa
     7261 gacgggggtc cggacgatga gccttttcga ggacgtggag ctgaacgacg cgcaggcggg
     7321 ggcggtcggc cgggcgatgc tcgccgtggc ccgcgcggac ggcgcggcag acccccgcga
     7381 ggtcgctctc atcgaggagc tcgctcccgt cgacacgtcc gcccccgacc cgactccggc
     7441 ggagctcgcc gagacgctga aggaggagaa agggcgcgag ctcctcctcc gttccgtcct
     7501 tctcgtcgcc ctcgtcgacg gcgactactc cgcggccgag cgggccgtcg tcggccggtt
     7561 cgccgaggcg ctcggcgttt ccggctcgcg cctcgaggag ctcgcgagcg gggtgaaggc
     7621 gttcctgatg gccccgctcc tcccgctcgc gaacacgtcg tcggtcctcg acgtctcgcg
     7681 caagctgaag gtctgagcgc ggcgcacggc gaaggagccg tgcccgaccc caccttccgt
     7741 ctcgcgcccg gcgccgggcg gcgcgtagac tttcgcgcca tgcgcgccca ccgcctccgc
     7801 ttcgcgctcg ccgcctcgtt cgtcgtctcc ctcgcgctcc cggcttccgc cgcccctcct
     7861 cccgcggagc acctcggcct cgccgtcggc tccgaccgga cgctcgccga ctggggccag
     7921 atcgtgtcgt acttccgcgc cctcgacgcg gcgtcggacc gcgtcgaggt gacgacgatc
     7981 gggacctcga cgcgcggcga ggagatgatc ctcgcggcga tctcctcgcc ggagaacctc
     8041 gcgaaccagc ggaggctccg cgaggtggcc cggcgcctcg ccgacccgcg cggcctttcg
     8101 cccgccgagg agaaggcgct cctcgtggaa gggaaggccg tcctcctcgt cacgtgcggc
     8161 atccacgcga gcgagatcgg cgcctcgcag atggcgatgg agtgggccca cgccctcgcc
     8221 accgccgagg acgccgagac gaagcggcgc ctctcggagg tggtcctcct cctcgtcccg
     8281 tcgctcaacc ccgacgggca ccggatggag gtcgactggt accggaagaa cctcggcacg
     8341 cggtgggagg ggagccggat gccgtggctc taccaccact acgtcgggca cgacaacaac
     8401 cgcgacttcg cgcagctgac gcaggccgag acgaaggcga tcagccgcgt cctctaccac
     8461 gactggttcc cgcaggtctt cctcgacgag caccagatgg ggacgagcgg cccgcggatg
     8521 ttcgtgccgc cgtacgccga cccgctcgac cccgacatcg acccgctcgt catccgggag
     8581 gtcaacatga tcgggtcgct catgtcgatg cggctcgagc aggcggggaa gagcggcgtc
     8641 atctcggcct gggcgttcga cgtctcctgg ccgggcggct cgaagaacac ggcgtggtgg
     8701 aagaacgtga cgggcgtcct gaccgaggtc gcctcggtcc gcctcgcgac gccgatcgag
     8761 gtcccgccga cggagctgcg cggcggcggg aaggggctcg tcgagtacgg gccccaggtc
     8821 aacttcccga acccctggcg gggcggaaag tggcgcctgc gcgacgtcat ggactacgag
     8881 cggatcgcct ccgacgcgat ccacgaatac tgcgccaccc accgcgagga cgtcctccgg
     8941 aacatgctcg cgaaggcgcg cgcctcgatc gcggccgccg cgcccggcga ggggtatcgc
     9001 atctcgatga cccagcgcga cccggcgacg gcgcgcgctg tggccgccct cctcgtcgag
     9061 cacggcgtcg aggtgaaggc cgattcggcc ggcgacgcgt gggtgccgct cgcgcagccc
     9121 tacggccgct tcgtccgcga gatgctcgag gtgcagcgat tccccgaggt gaagctcgtc
     9181 ccggggaagg agatcgtggc gccgtacgac gtcaccgcct ggagcctgcc gctctcgatg
     9241 ggcgtcaccg tggagagggg ccgcctcccg gagagcctgc gccccctcgg cgccgtcggg
     9301 ccggtaccga cactcccctc cggcaccacg gcagtcgccc tcggccccga gagcccggag
     9361 cgctggaagg tcgtcgcggc cgcccgaacg ggaggcgcgg tatcgatcct ttcggcggcc
     9421 gacggggcgc tcgccgcagg cacgatcgtc ctcgacgcgg ccgccgcaca ggcggcctcc
     9481 cgcgccgccg ccgacctcgg tctgtcgctc gcgccgctcg cggccgcacc ggccgccgcg
     9541 aggccattcc gggcgccgcg cgtcggcctc tacaagccgt ggatcgcctc gatggacgag
     9601 gggtggaccc gcttcctcct cgagcgctac ggcttcgcgc cgctgacgct cgacaacgcc
     9661 gcgctccgcg ccgggaagct cggcaccctc ttcgacgtga tcgtcctccc cgacgtcgaa
     9721 aaggaagtga tcgcggacgg gaagccgaaa cgggaggaag gggcgatgcg ctacttcccg
     9781 gacccgcggc ccgagtacgc gggcgggctc gggaaggagg gcgcagcggc gctccgcgcg
     9841 ttcgtggaag ggggcgggac gctcgtcgcg ctcggctcct ccacggagta cctcgtcgag
     9901 gagctcgggc tcccggtgcg caacgcgctc gcgcgcgcga agccggacga gttcctctgc
     9961 cccggcgggc tggtccggct cgacgtgagc cccgcgcacc cggtcacgtg gggactcccc
    10021 ccgtccgtcc ccggcttcct cgacggcccg ctcgccttcc agacgacgat cccgggggcg
    10081 gagatgacgc gcgaggtact cgcggcctat ccggccgacg ggcgggacgt cctcgtggcg
    10141 ggctggatcc gcggcgagga gaagctggcc cggaacgccg cggcggtcgc gctgacgctc
    10201 ggaaagggga aggtcgtcct cctcggcttc cgcccgcagc accgggcgca gacgaacgcg
    10261 acgttcccgt tcctcttcaa ctccctcgcc tggtcggtga tgtagcgtcg tccccgcgca
    10321 gctcgtcgcg gcgcgcgcgc gcctcggcct cgttcacccg gaggaggagg acgaggccgg
    10381 cgacgaagag gacggcgacg gagaggatcg cgaggcgcga ggtgccggtc gcctgccgca
    10441 ccgtcgcgaa gaggaacgtt ccgaggacgc ccgcgagctt gttgaagacg gagaagaagc
    10501 cgaagagctc ggccgacgac tcgcgcggga tcatcgcggc gaagagcgag cgggagagcg
    10561 cctggaccgc cccgaggacg aggcccgcga gcgccgcgag gacgacgaag ccggcgggcg
    10621 tcgtcaggag gtaagcccag ccgaccgcga cgagccagag cgcgacggcc aggacgagcg
    10681 cccgcttcgt cccccaggcg cccgcgaggc gcgcgaagag gagagccccg accatcgcga
    10741 ggagctggat gccgaggagc gtccccatca tcatcggcat cccgacgccg acctcctcgg
    10801 tgccgtagag cgaggccatc ttgatgacgg tctggacgcc gtcgttgtag aggacgaacg
    10861 cgaggaggaa gaggaggacc ggcttcacct tcgcgacgcg gcgcgtcgtc gcgagggtgt
    10921 cggcgacgcc ttcgcgcacc gcgcggagga gccccggttt cccgcccgtc cgcggaggtt
    10981 ccttcaggcc gaggaacgtg atcgccccga agccggccca ccagagcccg gcggagccga
    11041 gggcgatccg cgcggcggcc tccgccgaaa ggccccagcg cgcgtgcgtc gccatcagga
    11101 cgagcgcgag gacgaggtgg agcgagccgc cgacgtaccc gaccgcgaag cctcgcgccg
    11161 agaggcggtc gagctcctcg tctttcgcca ggtgcggcag gaaggagtcg tagaagacgt
    11221 tcgcggagac gaacgcgatc tgcgagaggg cgtagatcca gagggtcgtg gcgacgtctc
    11281 ccggcccgca cgccgcgagg agcatcgtca ggagcccccc ggcgaggccg aacacggtga
    11341 ggagcttccg tcgcgcgccg agccggtcgg cgatcgcgcc gaggatcggc gcggtcagga
    11401 agacggcaag cgtcgacgcg ctgttcacgt agccccagag gaccgtcgcg tcgacccgaa
    11461 tcccgaagag ctccacgccc cccttcggca cgactgtccc cgcgaagtag accggaagca
    11521 ccgccaccgc gaccgtcatc gcccaggcgg agttcgccca gtcgtaagcg agccacgccc
    11581 gcacctcact cttcctgccc ggcatggcgc gggagtctct cacggggacg ggcgcgggaa
    11641 gaggagagcc gagagacgga gagccgagag acggagagcc gagagacgga gagccgagag
    11701 acggagagcc ga
//
LOCUS       DAOTEZ010000084        11632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3881_length_11632_cov_7.796234, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000084 DAOTEZ010000000
VERSION     DAOTEZ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11632
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3881_length_11632_cov_7.796234"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="P5164_06315"
     CDS             <1..341
                     /locus_tag="P5164_06315"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRY43545.1"
                     /translation="FFVTSVTGVEILVYPLPVWRVFEEKLAALPFVHRARQKFLERYN
                     TYGQTVSLDGQGRLLIPALLRETSGVGSEVLVLAQADHLKVVDKTRHFAALSAAPITD
                     EDYDDLARAL"
     gene            354..1307
                     /gene="rsmH"
                     /locus_tag="P5164_06320"
     CDS             354..1307
                     /gene="rsmH"
                     /locus_tag="P5164_06320"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015751188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRY43546.1"
                     /translation="MELPAHVPVLLREVLEALRPERGGVLVDATLGLGGHAEALLDAS
                     PSVRLVGIDRDPEALALARARLARFGDRFVAVEGRHEELALHLDRLDLPQVDGVLADL
                     GVSSMQLDRAERGFSFMKDGPLDMRMGRTGPTAADLVAELPGEELARVFRDYGEEPRA
                     KAVARAVVSAREAAPIRTTGELRALVAKAVGGRREEGRDPATRVFQALRIATNRELVE
                     LERFLDDAIARLSLGARVAVLSYHSLEDRIVKDVFRDRAAGCTCPPSFPVCVCSRRRV
                     LALVTKKPIRPSDEEVLENRRSRSARLRVAERIAPGPPRSG"
     gene            1314..1742
                     /locus_tag="P5164_06325"
     CDS             1314..1742
                     /locus_tag="P5164_06325"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HRY43547.1"
                     /translation="MYTVRRPVDNVYLVREPDRRRTRELLALLLSPLPPLAVLFAAVW
                     TNLETFRLGYQIESLQKHKETLAEKQRQLEMERARVSALARVEEVARQRLGLVTPGRD
                     QVVFVRDGAFGAAPTAPVRHVPAGPELQGPPAPVRTEEGF"
     gene            1847..3841
                     /locus_tag="P5164_06330"
     CDS             1847..3841
                     /locus_tag="P5164_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HRY43548.1"
                     /translation="MRHDEFVKRAKRQQERTVELAPRRGTIVDREGRPLAVTTAVESV
                     FAIPSDIEDPAGVARTLAPIVKQPFRELKRKLSESEREFVWIARRVDAETAAAVKASA
                     LPGVRLLKESARRYPEGPLAAAVVGYVGTDSQGLGGLEYTWDREVRGRPARVTVLRDA
                     VQRSYAIRRGAPGGAGRSSDEVEGASLRLTLHAGLQHVAERELAAVRSETNARSASAV
                     VLDPATGEVLVMASVPTFDPNRWSEASADARRCRPIADAYEPGSTFKVITAAAAIEAG
                     TLRPDDLVDCGGGSLTIGRTTIREHGRAAWGALPLVDVLAHSSNVGAAHIGLGMGKTD
                     FHRAIRAFGFGRKTGLDLDGENGGLLRDPSAWSALSLPTISFGQEIGVNALQLARAFG
                     AIANGGVLPSPYLVADVSRPGLRSEARRPAPGARVLSEQTAAAMRRLLVKVVEEGTGR
                     KAAVPGFTVAGKTGTAQKAIPGAGYSSDRYVASFFGFLPAERPRLVVGVLVDEPRGRT
                     YGGDVAAPVFATIAGEAMRVLGEPGRPAEDRVTPTILTADLSRGASAAASPLPPGLVP
                     ASIRPTAVPEDLSRLAAGERLVPDVAGLSAREAVQQLAQRGFRPALSGHGFVVAQEPL
                     PGTPMARGSAVRLTLSFDPPSNDASLGALPFGDAAEAREP"
     gene            3838..5340
                     /locus_tag="P5164_06335"
     CDS             3838..5340
                     /locus_tag="P5164_06335"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRY43549.1"
                     /translation="MKLSVLVDGLPHRRVGPPGDPEVRAITHDSRRAGAGVLFAAFPG
                     KSADGRAFVGEAVLAGAPAAIGAAPAPDGVEVPYLEVESPRRAAGLLAARLAGDPSTR
                     LVMAGVTGTSGKTTTTLLLDEVLGAAHPKRGLFGTLVYRWGDAPAETLDASRTTPEAT
                     ELQPMLGALAAGGGTAATLECSSHALWLERLAGCAFDVAVFLNLTRDHLDDHGTMEAY
                     FEEKAKLFSMLKPGGRSVANVDDPWGRKLFARLPRETTLSFSLAAEADVTGRVLPGSD
                     GIRLRVERRATGEAFEVDSPLLGAPNAENLLAAATAALALGLPPAAIATRLGAFSAVP
                     GRLERVPNGLGLLVLVDYAHKPAALEGVLKTTRALAASRGGALRVVFGCGGDRDRGKR
                     PEMGRIAAELADHVVVTSDNPRSEEPAAIAAEVAAGSAAAGREPLVVLDRREAIHRAL
                     EAASPGDVVVVAGKGHETYQVTAGKKEPFDDRLVAREALAELERLRAGGA"
     gene            5337..6731
                     /gene="murD"
                     /locus_tag="P5164_06340"
     CDS             5337..6731
                     /gene="murD"
                     /locus_tag="P5164_06340"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRY43550.1"
                     /translation="MSLSLGPDLPEVSCAAVFGLAKSGKATVRALLERGVKVVATDSA
                     AAEALGTLPAGEGLSLVLGGHPASLLDGVDLCVVSPGVPMSIPALDAARASGIPVIAE
                     VELASRLVPGLVVGITGTNGKSTTTALTAALLSDAGRDAVACGNFGTPWIEYALGAAE
                     GAAPPAPSRVWVVELSSFQLEGLRRLAPDVAVHLNLTPDHLDRYRSVDDYGAAKARIF
                     ESQNEAQVAVLNADDPLVARIRTRARRLLFSRARAVEAGAWLKDAVFLADAAGKGPRV
                     YARREDLLLPGSHNVENALAALAATIPLGVTPESAARTFRTFKGLRHRTALVRTRKGV
                     AFYNDSKGTNVDATLKSLEGFADGSVFLILGGKDKGDDFRRLLPLAARKVRKVLAIGK
                     AGPAVMKALADGVACEEAGTIARAVEIGASEGRPGDVVLLSPACASFDQFRNFEHRGE
                     AFEELVLALPEAGG"
     gene            6733..7836
                     /gene="ftsW"
                     /locus_tag="P5164_06345"
     CDS             6733..7836
                     /gene="ftsW"
                     /locus_tag="P5164_06345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HRY43551.1"
                     /translation="MARKLAVDRLLFAPVVLLVGIGMLMIYSASAMQIYLPKAGGPSQ
                     PLYFVVKQGIAVLLGAALTVGALKVDYRFWNDLRVVRAGLALLVLALVAVLFLGPING
                     TRRWIDLGLLSIQPSEFGKIGLVVALAALLSRREGELDDLKRTLLPVAGLLAVLGGLV
                     LLQPDFGTALSYFAIAAAMLFYAGLRLRWFAGVATLALPALVAYAWSAPYRRARILAF
                     LNPEADIQGTGYQAYQSLIAVGTGGLTGLGLGDGRQKLFFLPYPYTDFIYAIVGEELG
                     LIGCAIVLGLFGMVFARAMRAAVNAPDPFGRLLGTGLAVMIVVQALVNISVVLALLPT
                     KGIPLPFISYGGSALWADLIAVGILLNVSQHAP"
     gene            7833..9221
                     /gene="murC"
                     /locus_tag="P5164_06350"
     CDS             7833..9221
                     /gene="murC"
                     /locus_tag="P5164_06350"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRY43552.1"
                     /translation="MNFLRVRRVHFVGIGGVGMSGLAEILASVGLTVTGSDLREGDET
                     RRLRDLGIPVFAAGHRAEHVAGADVVVYSSAVAEENPELVAARVAGVPVIKRAEMLAE
                     VMRVKTGVAIAGSHGKTTTTSMTGSILQAAGLDPTIVVGGRVRAMGTNARLGEGELLV
                     AEADEFDRSFLRLRPVVAVVNNIDLEHLDTYRDLADLKAAFTQFASTVPFFGAALLGL
                     DDPNVQEIRPALSTRVRVVTFGLTPQADVTARDLSLDRGGSRFTAIASGEVLGTLEVS
                     LPGLHNVKNALAAIAVSRELEVPFEACAKALSTFSGVARRFERKGERDGVLVVDDYAH
                     HPTEVAATLGAARQAHPERRLVVVFQPHLFTRTRDQADGFGAALLAADAVYVLPVYPS
                     REAPIPGVTSRLVSDAARSRGHRNVADLDDRTAAVARLEAELKDGDLLVTMGAGDVNR
                     VGEELLAAGGAA"
     gene            9218..10030
                     /locus_tag="P5164_06355"
     CDS             9218..10030
                     /locus_tag="P5164_06355"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HRY43553.1"
                     /translation="MSGAFYRPGTIRPPRAPRRGRPTRRFVLGLALLLLLVGSVAAAW
                     AAVHKHPRFLVTRVVLTGVPEPHRNEVEALSDPWIGRPLFSVDLEAAIADLSARPWVA
                     RAAARRVVPDTVALQVEPRAPVALARRDGELWTVDQNGLWLAAFSGGAGAPRFVVLDP
                     SGGASPDEAVARGARLLARLAADDPELHARVSEIVVLSEGFAVVDAPARLKLLLCDDA
                     LAPGRAFARWRAFLALAPELSRHGLLPREVDLRFENRIVLKAPGPEAARSDT"
     gene            10042..11286
                     /gene="ftsA"
                     /locus_tag="P5164_06360"
     CDS             10042..11286
                     /gene="ftsA"
                     /locus_tag="P5164_06360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRY43554.1"
                     /translation="MPKTSPYLVSLDIGSSKVCALIAETGSEGRVDVVGKGIATHKGT
                     RKGAIIDVPATIEAIKRATEEAEIMAGVQIERAVVGVAGPVVRSFNSRQVVAVAARNR
                     EISRDDIGRALDAARSCPIPSDYEILAVLQRQFVVDGQDGVADPLGMVGSRLEADVHV
                     VTVPVATTQNLLKCVNGAGVAVTEMVLEPLAASEAVLTPDEKDHGVVLADIGGGTTEI
                     AVWRQGALAHTAVIPIGGDLFTSDLAVLLKTPVPDAERLKKKYGAAVAGLAPAEETVD
                     VPRTGGREVHPVARSYMAEILEARARELLEVLWREATTDIPPQELRAGLVLTGGGAAL
                     DGMTDEAEQVLGVPVRIGTPRGLGGLVDVINGPEWACVAGLLLVGRGGAASPKGRPAA
                     PAGLLSKLKNSLGRMFTPASAI"
     gene            11350..>11632
                     /locus_tag="P5164_06365"
     CDS             11350..>11632
                     /locus_tag="P5164_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRY43555.1"
                     /translation="MGEEILTPARIKVVGVGGGGGNAVNRMIQAGIRGVEFIAANTDL
                     QVLRVNRAAQKIQLGISTSKGMGAGSNPEIGRTAALEDAEKIAEALAGSD"
BASE COUNT         1394 a         4508 c         4150 g         1580 t
ORIGIN      
        1 agttcttcgt cacgtcggtc accggcgtgg agattctcgt ctatccgctg ccggtgtgga
       61 gggtcttcga ggagaagctc gccgcgctcc cgttcgtcca ccgggcccgc cagaagttcc
      121 tcgagcgcta caacacctac ggtcagaccg tgagcctcga cgggcagggg cgcctcctga
      181 tcccggctct cctccgcgag acgtccggcg tcgggagcga ggtcctcgtc ctcgcgcagg
      241 ccgatcacct gaaggtcgtc gacaagacgc ggcacttcgc cgctctctcc gccgcgccga
      301 tcaccgacga ggactacgac gacctcgcgc gggcgctctg aggaagtgat ggggtggagc
      361 ttcccgctca cgttcccgtt ctcctccgcg aggtcctcga ggcgctccgt cccgagcggg
      421 gcggcgtcct cgtcgacgcg acgctgggtc tcggaggaca cgccgaggcg ctgctcgacg
      481 cgtccccgtc cgtacggctc gtcgggatcg accgcgaccc cgaggcgctc gcgctcgcgc
      541 gggcgcgcct cgcgcgattc ggcgaccgct tcgtcgccgt ggaggggcgc cacgaggagc
      601 tggccctcca cctcgaccgg ctggacctcc cgcaagtgga cggggtcctc gccgatctcg
      661 gcgtctcctc gatgcagctc gaccgggcgg agagggggtt ctctttcatg aaggacggtc
      721 ctctcgacat gcggatgggc cggacgggcc cgacggcggc cgacctcgtc gcggagctcc
      781 cgggcgagga gctcgcccgc gtcttccgcg actacggcga ggagccgcgc gcgaaggccg
      841 tggcccgcgc ggtcgtctcg gcgcgcgagg cggcgccgat ccggacgacc ggcgagctgc
      901 gcgcgctcgt cgcgaaggcg gtcggcggcc gccgcgagga gggccgcgac cccgcgacgc
      961 gcgtcttcca ggccctgcgc atcgccacga accgcgagct cgtcgagctc gagcgcttcc
     1021 tcgacgacgc catcgcgcgc ctctcgctcg gcgcccgggt cgcggtcctc tcctaccact
     1081 cgctcgagga ccggatcgtc aaggacgtct tccgcgatcg ggccgccggc tgcacctgcc
     1141 ccccctcctt ccccgtctgc gtctgctcgc gccgccgcgt cctcgcgctc gtgacgaaga
     1201 agccgatccg gccgtccgac gaggaggtcc tcgagaaccg gcgctcgcgc tcggcgcggc
     1261 tccgggtggc ggagcggatc gctcccgggc ctccgaggtc cggctgaggg gccgtgtaca
     1321 ccgtccggcg ccccgtcgat aacgtctacc tcgtccgcga gcccgaccga cggcggaccc
     1381 gggagctcct cgccctcctc ctgtcgccgc tgccgccgct cgccgtcctc ttcgcggcgg
     1441 tctggacgaa cctcgagacg ttccgcctcg ggtaccagat cgagagcctc cagaagcaca
     1501 aggagacgct cgccgagaag cagcgccagc tcgagatgga gcgggcgcgc gtctcggcgc
     1561 tcgcccgcgt cgaggaggtc gcacgccagc gcctcggcct cgtcacgccg ggccgcgacc
     1621 aggtcgtctt cgtgagggac ggagccttcg gcgcggcgcc gacggcgccg gtccggcacg
     1681 ttccggccgg gccggagctg caggggccgc ccgcgccggt ccggacggag gaggggttct
     1741 gacggccccc tccggccggg cgaacgcccg gcgcgcctac gtcctcgtcg ggttcgccgg
     1801 cctctggatc ctcgcgatcc tcgtccgcct cgtcgagctg cagctcgtgc ggcacgacga
     1861 gttcgtcaag cgagcgaagc gccagcagga gcggacggtc gagctcgcgc cgcgtcgtgg
     1921 gacgatcgtc gaccgcgagg ggcggccgct cgcggtgacg acggcggtcg agtcggtctt
     1981 cgcgatcccg tccgacatcg aagaccccgc cggcgtcgca cggaccctcg cgccgatcgt
     2041 gaagcagccc ttccgagagc tgaagcggaa gctctcggag agcgagcggg agttcgtctg
     2101 gatcgcacgc cgggtcgacg cggagacggc cgccgccgtg aaggccagcg ccctccccgg
     2161 cgtccggctc ctgaaggagt cggcgcggcg ctaccccgag gggccgctcg ccgccgcagt
     2221 cgtcggctac gtcgggaccg acagccaggg cctcggcggc ctcgagtaca cctgggaccg
     2281 cgaggtccgc ggccgccccg cccgcgtcac ggtcctgcgc gacgcggtgc aacgctccta
     2341 cgcgatccgg cggggcgcgc ccggcggcgc ggggcgctcc tccgacgagg tggagggggc
     2401 ctccctgcgg ctgacgctcc acgcggggct ccagcacgtc gccgagcggg agctcgcggc
     2461 ggtgcggtcc gagacgaacg cccgctccgc ctccgcggtc gtcctcgatc cggcgaccgg
     2521 ggaggtcctc gtgatggcct ccgtcccgac gttcgacccg aaccggtggt cggaggcgag
     2581 cgccgacgcc cgccgctgcc gcccgatcgc cgacgcctac gagccgggct ccacgttcaa
     2641 ggtgatcacc gccgcggccg ccatcgaggc cgggacgctt cgcccggacg acctcgtcga
     2701 ctgcggcggc ggctcgctga cgatcggccg gacgacgatc cgagagcacg ggcgggcggc
     2761 ctggggcgct ctcccgctcg tcgacgtcct ggcccactcc tcgaacgtcg gcgcggcgca
     2821 catcggcctc gggatgggga agacggactt ccaccgcgcg atccgcgcgt tcggcttcgg
     2881 ccggaagacg ggcctcgacc tggacggtga gaacggcggg ctcctgcgcg acccgtcggc
     2941 ctggagcgcg ctctcgctcc cgacgatctc gttcgggcag gagatcggcg tgaacgccct
     3001 ccagctcgcg cgtgccttcg gggcgatcgc gaacggcggc gtcctcccct cgccgtacct
     3061 cgtcgccgac gtctcccggc ccggcctgcg gtcggaggcc cgccggcctg ccccgggcgc
     3121 gcgcgtcctc tccgagcaga cggccgcggc gatgcggcgg ctcctcgtga aggtcgtcga
     3181 ggaagggacg ggccggaagg ccgccgtccc cggcttcacg gtggcgggga agaccggcac
     3241 ggcccagaag gcgatcccgg gcgcgggcta ctcgtccgac cgctacgtcg cctcgttctt
     3301 cggcttcctc ccggccgagc ggccgcggct cgtcgtcggc gtcctcgtcg acgagccgcg
     3361 gggccggacc tacggcggcg acgtcgccgc ccccgtcttc gccacgatcg cgggcgaggc
     3421 gatgcgggtc ctgggcgagc cgggtcgccc ggccgaggac cgcgtcaccc ccacgatcct
     3481 gacggccgac ctctcgcgcg gcgcctccgc ggccgcgagc ccgctcccgc ccggcctcgt
     3541 cccggcttcg atccggccga ccgccgtccc cgaggacctc tcgcgcctcg ccgcgggaga
     3601 gcgcctcgtc cccgacgtcg ccgggctgtc ggcgcgggag gcggtccagc agctcgcgca
     3661 gcgcgggttc cggccggcgc tttccgggca cggcttcgtc gtcgcccagg agccgcttcc
     3721 cggaacgccg atggcgcgcg gcagcgccgt ccggctcacg ctctccttcg accctccgtc
     3781 gaacgacgcc tccctcgggg cgctcccgtt cggcgacgcc gccgaggcgc gcgagccgtg
     3841 aagctctcgg tcctcgtcga cggccttccg caccgccgcg tcggcccccc gggcgacccg
     3901 gaagtgaggg cgatcacgca cgactctcgc cgcgccggcg ccggcgtcct gttcgccgcc
     3961 ttcccgggca agtcggccga cgggcgcgcg ttcgtcggcg aagcggtcct cgccggggcg
     4021 ccggcggcga tcggagccgc gcccgcgccg gacggcgtgg aggtgcccta cctcgaggtg
     4081 gaatcgccgc ggcgggccgc cggcctcctc gccgcacgcc tcgcgggcga tccgtccacg
     4141 cggctcgtga tggcgggggt caccgggacg agcggcaaga cgacgacgac gctcctcctc
     4201 gacgaggtcc tcggcgccgc gcacccgaag cggggcctct tcggcacgct cgtctaccgc
     4261 tggggcgatg cccccgccga gacgctcgac gcctcccgga cgacgccgga ggcgaccgag
     4321 ctccagccga tgctcggcgc gctcgccgcc ggcggcggca cggccgcgac gctcgagtgc
     4381 tcctcgcacg ccctctggct cgagcggctc gcggggtgcg cgttcgacgt cgccgtcttc
     4441 ctgaacctga cgcgcgacca cctcgacgac cacgggacga tggaggccta cttcgaggag
     4501 aaggcgaagc tcttctcgat gctcaagccg gggggccggt cggtcgcgaa cgtcgacgac
     4561 ccgtgggggc ggaagctctt cgcgcggctt ccgcgcgaga cgacgctctc cttctcgctc
     4621 gcggccgagg ccgacgtgac cggacgcgtc ctcccgggga gcgacggcat ccgcctccgc
     4681 gtcgagcggc gcgccacggg ggaggcgttc gaggtcgact cgccgctcct cggggcgccg
     4741 aacgccgaga acctcctcgc ggccgcgacg gcggccctcg cgctcggcct cccgccggcc
     4801 gcgatcgcga cgcgcctcgg cgcgttctcc gcggtccccg ggcggctcga gcgcgtgccg
     4861 aacgggctcg gcctcctcgt cctcgtcgac tacgcccaca aaccggccgc gctcgaaggg
     4921 gtcctgaaga cgacgcgcgc gctcgcggcc tcgcgcggag gcgcgctgcg ggtcgtcttc
     4981 ggctgcggcg gcgaccgcga ccgggggaag cgccccgaga tggggcggat cgccgccgag
     5041 ctcgccgacc acgtcgtcgt cacctccgac aacccgcgct ccgaggagcc ggccgcgatc
     5101 gcggcggagg tcgccgcggg ctccgcggcg gcggggcgcg agccgctcgt cgtcctcgac
     5161 cgacgcgagg cgatccatcg cgcgctcgag gcggcctcgc cgggcgacgt cgtcgtcgtc
     5221 gccggcaagg ggcacgagac ctaccaggtg accgccggaa agaaggagcc gttcgacgac
     5281 cggctcgtgg cccgcgaggc gcttgcggag ctggagcggc tccgggcggg gggcgcgtga
     5341 gcctctccct cggaccggac ctccccgagg tctcctgcgc cgccgtcttc ggcctcgcga
     5401 agtcggggaa ggcgaccgtc cgcgcgctcc tcgagcgggg cgtgaaggtc gtcgccaccg
     5461 actccgctgc ggccgaggcg ctcgggacgc tcccggccgg cgaggggctc tcgctcgtcc
     5521 tcggcggcca tcccgcgtcg ctcctcgacg gggtcgacct ctgcgtcgtc tcgccggggg
     5581 tcccgatgtc gatcccggcg ctcgacgccg cgcgcgcctc cggcatcccc gtgatcgccg
     5641 aggtcgagct cgcctcgcgg ctcgtccccg gcctcgtcgt cggcatcacc ggaacgaacg
     5701 gcaagtcgac gacgacggcg ctcaccgccg cgctcctcag cgacgcgggg cgggacgccg
     5761 tcgcctgcgg gaacttcggc acgccctgga tcgagtacgc cctcggcgcg gccgaggggg
     5821 ccgctccgcc ggcgccgagc cgcgtctggg tcgtcgagct ctcctcgttc cagctcgagg
     5881 ggctccggcg cctcgccccc gacgtcgccg tccacctgaa cctcaccccc gaccacctcg
     5941 accgctaccg ctccgtcgac gactacggcg cggcgaaggc gcggatcttc gagagccaga
     6001 acgaggcgca ggtcgccgtc ctgaacgccg acgacccgct cgtcgcccgc atccggacgc
     6061 gggcgcggcg cctcctcttc agccgtgcgc gggccgtcga ggccggcgcc tggctgaagg
     6121 acgccgtctt cctcgcggac gccgccggga agggtccgcg cgtctacgcc cggcgcgaag
     6181 acctcctcct ccccggctcg cacaacgtcg agaacgcgct cgccgccctc gccgcgacga
     6241 tcccgctggg cgtcacgccc gagagcgcgg cccggacctt ccggacgttc aagggcctcc
     6301 ggcatcgcac ggccctcgtg aggacccgga agggcgtcgc cttctacaac gactcgaagg
     6361 ggacgaacgt cgacgcgacg ctgaagagcc tcgagggctt cgccgacggg agcgtcttcc
     6421 tgatcctcgg cggcaaggac aagggggacg atttccggcg cctcctcccg ctcgcggcgc
     6481 ggaaggtgcg caaggtcctc gcgatcggca aggcgggtcc ggcggtgatg aaggccctcg
     6541 cggacggcgt cgcctgcgag gaggccggga cgatcgcacg ggccgtcgag attggggcgt
     6601 cggaggggcg gcccggcgac gtcgtcctcc tgtcgcccgc ctgcgcttcg ttcgaccagt
     6661 tccgcaactt cgagcaccgg ggcgaggcct tcgaggagct cgtcctcgcg ctccccgagg
     6721 cgggaggctg acgtggcgcg caagctcgcg gtcgaccggc tcctcttcgc ccccgtcgtc
     6781 ctcctcgtcg ggatcgggat gctgatgatc tacagcgcct cggcgatgca gatctacctc
     6841 ccgaaggccg gcggtccctc ccagccgctc tacttcgtcg tgaagcaggg gatcgcggtc
     6901 ctcctcggcg cggccctcac ggtcggagcc ctgaaggtcg actaccggtt ctggaacgac
     6961 ctgcgcgtcg tccgggccgg cctcgcgctc ctcgtcctcg cgctcgtcgc cgtcctcttc
     7021 ctcgggccga tcaacggcac ccgccgctgg atcgacctcg ggctgctctc gatccagccc
     7081 tccgagttcg ggaagatcgg cctcgtcgtc gcgctcgcgg cgctcctctc ccggcgcgag
     7141 ggggagctcg acgacctgaa gcggacgctc ctcccggtgg ccggcctcct tgccgtcctg
     7201 ggcgggctcg tcctcctgca gcccgacttc gggacggcgc tctcgtactt cgcgatcgcg
     7261 gcggcgatgc tcttctacgc cgggctgcgc ctccgctggt tcgccggggt ggccacgctc
     7321 gcccttccgg cgctcgtcgc ctacgcctgg tcggccccct accgccgggc ccggatcctc
     7381 gccttcctga acccggaggc ggacatccag gggaccggct accaggccta ccagtcgctc
     7441 atcgccgtcg gcaccggcgg cctgaccggg ctcggtctcg gcgacggccg tcagaagctc
     7501 ttcttcctcc cctaccccta caccgacttc atctacgcga tcgtcgggga ggagctcggc
     7561 ctgatcggct gcgcgatcgt cctcggcctc ttcgggatgg tcttcgcccg cgcgatgcgc
     7621 gccgccgtga acgcgccgga ccccttcggc cggctcctcg gcaccgggct cgccgtgatg
     7681 atcgtcgtcc aggccctcgt gaacatctcc gtcgtcctcg ccctcctgcc caccaagggg
     7741 atcccgctcc cgttcatctc gtacggcggc tccgccctct gggcggacct gatcgcggtc
     7801 gggatcctcc tcaacgtctc gcagcacgcg ccgtgaactt cctccgcgtc cgccgcgtcc
     7861 acttcgtcgg gatcgggggg gtcgggatgt ccggcctcgc cgagatcctc gcgagcgtcg
     7921 ggctgaccgt caccggctcc gacctgcgcg aaggggacga gacccggcgc ctgcgcgacc
     7981 tcggcatccc cgtcttcgcc gccggccacc gggccgagca cgtcgcggga gcggacgtcg
     8041 tcgtctactc ctcggccgtt gccgaggaga accccgagct cgtggcggcg cgggtcgccg
     8101 gggtcccggt catcaagcgg gccgagatgc tcgccgaggt gatgcgggtg aagaccggcg
     8161 tcgcgatcgc gggctcgcac ggcaagacga cgacgacctc catgacgggc tcgatcctcc
     8221 aggccgccgg cctcgacccg acgatcgtcg tcggcgggcg ggtccgtgcg atggggacga
     8281 acgcacgcct cggcgagggc gagctcctcg tcgccgaggc ggacgagttc gaccggtcgt
     8341 tcctgcgcct gcggcccgtc gtggcggtcg tcaacaacat cgacctcgag cacctcgaca
     8401 cctaccgcga cctcgccgac ctcaaggccg ccttcacgca gttcgcgtcg accgtcccct
     8461 tcttcggcgc ggcgctcctc ggcctcgacg acccgaacgt ccaggagatc cgccccgccc
     8521 tctcgacgcg cgtgcgggtg gtgacgttcg ggctgacgcc gcaggccgac gtgacggcgc
     8581 gcgacctctc gctcgaccga ggcggctcgc gcttcacggc gatcgcctcg ggggaggtcc
     8641 tcgggacgct cgaggtctcg ctccccggcc tccacaacgt caagaacgcc ctggcggcga
     8701 tcgccgtctc gcgtgagctc gaggtgccgt tcgaggcgtg cgccaaggcg ctctcgacct
     8761 tctccggggt cgcgcggcgc ttcgagcgga agggggagcg ggacggcgtc ctcgtcgtcg
     8821 acgactacgc ccaccacccg accgaggtcg cggccacgct cggggcggcc cgccaggcgc
     8881 acccggagcg gcgcctcgtc gtcgtcttcc agccgcacct cttcacgcgg acccgcgacc
     8941 aggccgacgg cttcggcgcg gcgctcctcg cggccgacgc cgtctacgtc ctcccggtct
     9001 acccgtcccg cgaggcgccg atcccgggcg tcacgtcgcg cctcgtctcc gacgcggccc
     9061 gctcgcgagg gcaccgcaac gtcgccgacc tcgacgaccg caccgccgcg gtggcgcggc
     9121 tcgaggcgga gctgaaggac ggcgacctcc tcgtgacgat gggcgcgggc gacgtgaacc
     9181 gggtcggcga ggagctcctc gcggcgggag gggcggcgtg agcggcgcct tctaccgccc
     9241 cgggacgatc cggccgccgc gggcgccgcg gagggggcgg ccgacgcgcc ggttcgtcct
     9301 cggcctcgcc ctgctcctcc tcctcgtcgg gagcgtggcg gcggcctggg cggcggtcca
     9361 caagcacccg aggttcctcg tcacgcgcgt cgtcctgacg ggagtccccg agccgcaccg
     9421 gaacgaggtg gaggcgctct ccgacccgtg gatcgggcgg cccctcttct ccgtcgacct
     9481 cgaggcggcc atcgccgacc tctcggcgcg cccctgggtg gcgcgggccg cggcccgccg
     9541 cgtcgtcccc gacaccgtcg ccctccaggt cgagccgcgg gcgccggtcg ccctcgcccg
     9601 gcgggacggc gagctctgga ccgtcgacca gaacggcctc tggctcgcgg ccttctcggg
     9661 cggagcggga gccccgcgct tcgtcgtcct cgacccctcg ggcggcgcgt cgccggacga
     9721 ggcggtcgcg cgcggcgcgc gcctcctcgc gcggctcgcc gcggacgacc cggagctcca
     9781 cgcccgcgtc tccgagatcg tcgtcctctc cgaagggttc gccgtcgtcg acgcgccggc
     9841 gcgcctgaag ctcctcctct gcgacgacgc cctggcgccg gggcgggcct tcgcccgctg
     9901 gcgcgcgttc ctcgccctcg cccccgagct ctcgcgtcac ggcctcctcc cgcgggaggt
     9961 cgacctgagg ttcgagaacc gcatcgtcct gaaggccccg ggccccgaag ccgcccggag
    10021 cgacacctga ggaaaggacc cgtgccgaag accagcccgt acctcgtcag cctcgacatc
    10081 ggatcctcga aggtctgcgc gctcatcgcc gagaccggga gcgaggggcg cgtcgacgtc
    10141 gtcggcaagg ggatcgccac ccacaagggg acccgcaagg gggcgatcat cgacgtcccg
    10201 gccacgatcg aggcgatcaa gcgcgccacg gaagaggccg agatcatggc gggcgtccag
    10261 atcgagcgcg ccgtcgtcgg cgtcgccggc cccgtcgtga ggtcgttcaa cagccggcag
    10321 gtcgtcgcgg tcgccgcgcg caaccgcgag atctcccgcg acgacatcgg ccgcgcgctc
    10381 gacgccgccc gctcctgccc gatcccctcc gactacgaga tcctcgccgt cctccagcgc
    10441 cagttcgtcg tcgacgggca ggacggcgtc gccgacccgc tcggcatggt cgggagccgc
    10501 ctcgaggcgg acgtccacgt cgtcaccgtc cccgtcgcca cgacgcagaa cctcctgaag
    10561 tgcgtcaacg gagcgggggt cgcggtcacg gagatggtcc tcgagccgct cgcggcgagc
    10621 gaggccgtcc tgacgcccga cgagaaggac cacggcgtcg tcctcgccga catcggcggc
    10681 gggacgacgg agatcgcggt ctggcggcag ggggccctcg cgcacacggc cgtcatcccg
    10741 atcggcgggg acctcttcac gagcgacctc gcggtcctcc tgaagacgcc cgtccccgac
    10801 gcggagaggc tgaagaagaa gtacggcgcc gccgtcgcgg gccttgcccc cgccgaggag
    10861 acggtcgacg ttccgcggac cggagggcgc gaggtccacc cggtcgcgcg gagctacatg
    10921 gcggagatcc tcgaggcgcg cgcccgcgag ctcctcgagg tcctctggcg cgaggcgacg
    10981 accgacatcc cgccgcagga gctgcgcgcc ggcctcgtcc tgacgggcgg cggagccgcg
    11041 ctcgacggta tgaccgacga ggccgagcag gtgctcggcg tcccggtccg gatcggcacc
    11101 ccgcgcgggc tgggcggcct cgtcgacgtc atcaacgggc cggagtgggc gtgcgtggcg
    11161 gggctcctcc tcgtcggacg cggcggggcc gcctcgccga agggccgccc cgcggcgccc
    11221 gcggggctcc tgtcgaagct gaagaactcg ctggggcgga tgttcacccc ggcgtcggcc
    11281 atctgaaggg acctggagga agagatgatt cttttcgagg acgaggggag cacgacgcct
    11341 gccatcacca tgggcgagga gatcctcacc ccggcgcgga tcaaggtcgt gggcgtcgga
    11401 ggcggcggcg gaaacgccgt gaaccggatg atccaggccg gcatccgggg cgtggagttc
    11461 atcgccgcga acaccgacct ccaggtgctg cgggtcaacc gcgcggcgca gaagatccag
    11521 ctcgggatct cgacctcgaa ggggatgggg gccggatcga acccggagat cggccggacc
    11581 gccgccctcg aggacgccga gaagatcgcc gaggcgctcg ccggctccga ca
//
LOCUS       DAOTEZ010000085        11607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3892_length_11607_cov_5.539733, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000085 DAOTEZ010000000
VERSION     DAOTEZ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11607
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3892_length_11607_cov_5.539733"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /gene="hybB"
                     /locus_tag="P5164_06370"
     CDS             complement(<1..1197)
                     /gene="hybB"
                     /locus_tag="P5164_06370"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HRY43556.1"
                     /translation="MPKLALPRLTFWRGVLLAILAAGTYATFLRVFKGLAGSTNLTDA
                     FPWGLWIGFDILCGVALAAGGFTISATVYIFNLEKFRPIVRPAILTAFLGYVLVVVAL
                     LFDLGRPWNVWHPLVMWNPHSVMFEVGWCVTLYTTVLALEFSPVVFERFKMTKTLHVL
                     HVIMPILVVLGVLLSTLHQSSLGSFYLIVPTKLHALWYSPLLPVYFFLTALTLGCAMT
                     IVESFLSHRAFGKELEMELLVPLGRVALLILGVTAVIRFADFRHRGILGLPFAPTYES
                     RLFLAEMIIGVLAPAVLLALPKVRQHRTGLFLSALLIVMGVVMNRLNVSITGMERSSG
                     EIYVPAFTEIAITLSVVGVGFLVFALAVKYLPVFPEHEMKDARPPVAPEGGSVAAQPI
                     RSLPMPA"
     gene            complement(1208..1984)
                     /locus_tag="P5164_06375"
     CDS             complement(1208..1984)
                     /locus_tag="P5164_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRY43557.1"
                     /translation="MLNTQAKGLLLDTTRCIGCGACALACKERNGLPKTSDVVLEDKL
                     SDKTFSTVNQVGNRYVRRMCMHCATPSCASACPVAALEKTAAGPVVYHESKCMGCRYC
                     MIACPFGIPKYEWEKKAPLVRKCDLCADRLAKGLKTACATACPTGATTFGNRDALVLE
                     ARARFAANPGRYREHVYGFEEVGGTSVLLISDAKPEELGYPAGLDETPPPHLTWRVLN
                     IIPDIVMTGAVLLGGIYWIRNRRDEVEAAEGHHDASTKGH"
     gene            complement(2319..5639)
                     /locus_tag="P5164_06380"
     CDS             complement(2319..5639)
                     /locus_tag="P5164_06380"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRY43558.1"
                     /translation="MSSRPLLRPLLAATFAALLLSAPPGLADADGPPEARLLRFPDVR
                     GDTVVFVYGGDLWRVPLSGGAAFRLTSHPGLELFPKLSHDGKWIAFSAEYTGTRQVWV
                     VPAHGGTPRQLTFATDVGPMPPRGGWDNWVLGWTKDGKVLVRMNRVPWNGRMGRYFLV
                     DPKGGLETALEIPEGGSASLSPDGARLAYCPVDREFRTWKRTKGGRAQDVWLYDFAAK
                     RSERLTTWEGTDNFPMWWGDAVYFTSDRERTLNLWRYDLKTRETRKLTSFTEFDVLWP
                     SLGDGRIVFMNGGDLWTLDLATEKAARIPIRLGSDAAATVPAFRDVAGNVAGMALSPS
                     GARVVLDARGDLYSLPAKDGVVRPLTTGSAVRERDPAWSPDGKWIAYLSDETGEYDLW
                     IRPQDGTGTPRRLTTDGAPWKHAPAFSPDGKKIAFGDRAQRLRVVDVASGKVTEVDRS
                     GFEDIDVYSFSPDSRWLVYEKSHSTRLPGLWAAPLEGGKPIRLGDGLTADFSPAFSAD
                     GKYLFFLSNRDYAPTFSAFEFSYVYPKATRVYAAALSPDVPHLFPLKSDEEKGTAGEA
                     EAAAKPASGKADGGKDGKKDDAKSGKAAEPSRVVVLAEGFAARTIALPGVKPGDLRSL
                     RATEEAVYYVRDGEDEGALFRFDLKERKEEKVLDGVSAYRLSADGKKLLWRSGKELGV
                     ADARAGLASGAGKLDLSGLRTKLDLPAEWAQIWRDGARTVRDWFYDPGLHGVDWNALV
                     ARYGALVPFVAHRADLDFLLGELVSELEAGHTYVTSGDEPKVERVPGGFLGCELTADP
                     SGRYRIARIFPGENWDEPFRSPLTEPGVNVAEGELLLAIDGVDLTTADNPYRLLEGKA
                     GRAVTLLVGKAPSREGGRTVTVRTVASEQGLRYLDWVKSRMALVEKLSGGKVGYIHLP
                     DTALAGNRMLQKLFYSQASKPALLVDDRYNGGGFIPDRMIEMLSRTTLAFWARRGVDA
                     MRTPGFAHDGPKAMLVNSYSASGGDALPYFFRKEKLGPIVGTRTWGGLIGLSGNAEFV
                     DGGSVDVPTFRIYDDDGKWVVENEGVAPDVEVFDVAERRLDGGDPSLEKGVEILLQEL
                     AKRPATRPAAPVPPRMSN"
     gene            5765..6883
                     /locus_tag="P5164_06385"
     CDS             5765..6883
                     /locus_tag="P5164_06385"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRY43559.1"
                     /translation="MAFLKVLESPDPALKDQTIPLAGKEVLIGRGKENGLVLEDHAVS
                     RHHARIVEEAEGHLLVDNNSANGSWVNGQRVTEARLKPGDQIRLGKTLFEIQGVYDPE
                     GTALLDVQGMGMPMKPSAPPAAPASPPPPAPAPPQVAAPPPAPRPAPVETPTVPEPVP
                     MRRPSPPPTPIPPPTPVPAPRPAPPPAPVAARPAPPPAPMAARPAPMAPPPAPAAAPG
                     YYDEAAPVEGEPAGFWIRFLASLIDSVILNILLAIVWAPTFFITFRAAASGEAPGALA
                     AILPVFSFLLTMVLSLGYTLWFWANKGATPGKKMLGLRIVREDGEEPLGWGTAFMRLV
                     GYMVSGFILYIGFFMIAFNPEKQGLHDKIAKTRVLKVR"
     gene            complement(6974..>7376)
                     /locus_tag="P5164_06390"
     CDS             complement(6974..>7376)
                     /locus_tag="P5164_06390"
                     /inference="COORDINATES: protein motif:HMM:NF025475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HRY43560.1"
                     /translation="APAPAPAPAPRHPAAERPSPAVAAIGGLVGDDANAFKGLLDFKF
                     ETLVAPKLIRVLYLVFAALCALGALGGIISGLKTVVTGFRFDSFFVIVTGLVMTVLAP
                     VLAVVYLSLMRVVFEGVKVAFLVKEKLESKG"
     assembly_gap    7377..7476
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7477..7677)
                     /locus_tag="P5164_06395"
     CDS             complement(<7477..7677)
                     /locus_tag="P5164_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43561.1"
                     /translation="MPPEIFFVAVDGRQLDGEHDMSQVRQLCTQYAGRQVMVWNEALG
                     SWTDPKTLPSFRVAAAPAPAPAP"
     gene            complement(7689..10541)
                     /locus_tag="P5164_06400"
     CDS             complement(7689..10541)
                     /locus_tag="P5164_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRY43562.1"
                     /translation="MRRTALPASAALAAALLAAAPGFAAPRGAEMSRDVLTIPATETT
                     LPNGLKVIVVPTGFPNLVSLQIPVQTGSRNEVEPGKSGFAHFFEHMMFRGTKSYPPEE
                     YQAILTRAGASQNAYTTDDYTNYHTTFAKEDLEKVLEIEADRFRNLEYPEAAFKTESR
                     AVLGEYNKNSANPVSKLFEAMRDAAFTTHTYKHTTMGFLRDIEDMPNQFAYSRQFFDR
                     WYRPEYTTILVAGDVDPAKVLPLVEKYWGSWTRGSWSVEIPREPAPSGPKSAHVPWPT
                     PTLPWVVVSFRGPAFSTTEKDWAALEVLSDLSFGPTSAVYKKLVETEQKADQLFAYFP
                     ASADPQLLMVAARVKKLEDAAAVRDELLRTFAALRAEPVPARRLADAQSRARYALVRS
                     FDNTEAIASTLAQYVRHRREYGTLNELYRLYEALTPEDLRAAAAKYVVDAGLVQTTLS
                     HEPLPEPLAKLPALGSLAPAVAAPEGLSVVERPSSHPQVTFKLLFSAGSAHDPKGKEG
                     LAALTAAMVAEAGSKDAGIDEIRKALFPIAASFSAGVDREMTVFTGSVHRDNLASYLD
                     VALPQLLDPGFREEDFRRLKDAQRNALAQDLIANNEEELGKERLQSVVFAGTPYAHPP
                     LGTLGGIDAIALDDVKAFWKAAYTRAALTVGLAGDVPADLKARLLSRLGELAAGPALP
                     APAGVKGRRPPALEVEIVQKETRATAISFGLPIEVTRSHPDFAALSVFRAFLGEHRNS
                     MAHLYQRIRELRGMNYGDYAYIEAFPYGMYATSPAPNVARRAQLFEVWIRPVVPANGP
                     MALKIALHELDRLIRKGISEADFQRTRDYLMKNVYLLTSTQGDDLGAALDSRWYGTAD
                     LVPYLRERLAKLTRADVNRAIRKHLSAETLSVVVITKDAEGLKKQLLSSEPSTIAYDA
                     PKPPEVLTEDAVIGARKLGLTPERVRITPVSEVFAR"
     gene            complement(10600..>11607)
                     /locus_tag="P5164_06405"
     CDS             complement(10600..>11607)
                     /locus_tag="P5164_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HRY43563.1"
                     /translation="KGLAAREPVPAETGFRIVTSGNGLEAAKAAREALLAGTDPLDAA
                     IAGVNVVELDPADLTVGYGGIPNEEGVVELDAAVMDGRTMKAGAVAALTGVKTPSKVA
                     KLVMERTDRVFLVGAGARRFATAHGFPDEELLTEKTRKIWLYWKNRLSDLDDWIEAAK
                     DAEDPDVKEFVRKYGKGIFRPQGTIHMSVRSASGDLVGVTTTSGLFFKIPGRVGDSPV
                     IGAGLYSENGVGSAGSTGRGEANLLTCGSHTVVEHLRDGLAPEEACLKALRRIAEKTH
                     HVPRHRDAKGRPTFNVQFYCVDAAGRTAGAAIWSGSKYVVGDAAGVRTLDCAYLHERD
                     A"
BASE COUNT         1682 a         3766 c         4354 g         1705 t
ORIGIN      
        1 cgccggcatc ggaagggagc ggatcggctg ggcggcgacg ctgcccccct cgggcgcgac
       61 gggcgggcgg gcgtccttca tctcgtgctc ggggaagacc gggaggtact tcacggcgag
      121 ggcgaagacg aggaagccga cgccgacgac cgacagggtg atggcgatct cggtgaaagc
      181 ggggacgtag atctcgcccg aggagcgctc catgccggtg atcgagacgt tcaggcggtt
      241 catcacgacg cccatgacga tgaggagcgc cgagaggaag agcccggtcc ggtgctggcg
      301 gaccttcggc agcgcgagca ggacggccgg ggcgaggacg ccgatgatca tctcggcgag
      361 gaagagccgc gactcgtagg tcggggcgaa aggcaggccg aggatcccgc ggtgccggaa
      421 gtcggcgaag cggatcaccg ccgtgacgcc gaggatgagg agcgcgaccc gcccgagcgg
      481 gacgaggagc tccatctcca gctccttgcc gaacgcgcgg tgcgagagga acgactcgac
      541 gatcgtcatc gcgcagccga gcgtgagggc ggtgaggaag aagtagacgg ggaggagcgg
      601 cgagtaccag agggcgtgga gcttcgtcgg gacgatcagg tagaacgacc cgagggagga
      661 ctggtggagc gtcgagagga ggacgccgag gacgacgagg atcggcatga tcacgtgcag
      721 gacgtgaagc gtcttcgtca tcttgaagcg ctcgaagacg accgggctga actcgagcgc
      781 gaggaccgtc gtgtagagcg tcacgcacca cccgacctcg aacatgaccg agtgggggtt
      841 ccacatgacg agcgggtgcc agacgttcca gggtcgcccg aggtcgaaga ggagcgcgac
      901 gacgacgagg acgtagccga ggaacgccgt caggatcgcg ggcctcacga tcggccggaa
      961 cttctcgagg ttgaagatgt agacggtggc gctgatcgtg aagccgccgg cggcgagggc
     1021 gacgccgcag aggatgtcga acccgatcca gaggccccag gggaacgcgt cggtcaggtt
     1081 cgtggagccg gcgagcccct tgaagacgcg gaggaaggtg gcgtaggtcc cggcggcgag
     1141 gatcgccagg aggacgccgc gccagaacgt gaggcgggga agcgcgagct tcggcatgtc
     1201 gctctcctca gtgccccttc gtcgaggcgt cgtggtggcc ttcggccgcc tcgacctcgt
     1261 cgcggcggtt tcggatccag tagatgccgc cgaggaggac ggcgccggtc atgacgatgt
     1321 cggggatgat gttgaggacc cgccaggtca ggtgcggcgg cggagtctcg tcgaggccgg
     1381 ccgggtagcc gagctcctcg ggcttcgcgt cggagatcag gaggaccgac gtccccccga
     1441 cctcctcgaa gccgtagacg tgctcgcggt agcggccggg gttcgcggcg aagcgcgcgc
     1501 gcgcctcgag gacgagggcg tcgcggtttc cgaacgtcgt cgcgccggtg gggcaggcgg
     1561 tcgcgcaggc cgtcttcagc cccttcgcga ggcggtcggc gcagaggtcg cacttgcgca
     1621 cgagcggcgc cttcttctcc cactcgtact tcgggatgcc gaaggggcag gcgatcatgc
     1681 agtagcggca gcccatgcac ttcgactcgt ggtagacgac cggtccggcc gcggtcttct
     1741 cgagggcggc gaccgggcag gccgatgcgc acgacggcgt cgcgcagtgc atgcacatgc
     1801 gccgcacgta ccggttcccg acctggttca ccgtcgagaa cgtcttgtcg gacagcttgt
     1861 cctcgaggac gacgtccgag gtcttcggga gcccgtttcg ctccttgcag gcgagcgcgc
     1921 acgcgccgca gccgatgcag cgcgtcgtgt ccaggagcag ccccttcgct tgcgtattca
     1981 acatgggcct ctcgtacccc cccgggcgaa cttcaggtcc gccccgtaga ggatttcgca
     2041 agcgtcgggc cagtgtgttg aggaattcgt aattagctct acagaagtag tttagcgctg
     2101 gctacgccga tccgcccagg aaggtgagtt gagaaattca acgccacgat ttcggaccga
     2161 tggcataagc cgcccatttt caacgagata cgtttgggcc gggagacgtc tcgatttgag
     2221 ccgaagcgtt gagaatttct acgcgctggg gcgatttccg acacgaagga gccggtggcg
     2281 tggagtttct acacgccacc ggccctgcga gaccggactc agttggacat gcgaggagga
     2341 acgggcgccg cgggccgggt cgccggccgc ttcgcgagct cctggaggag gatctccaca
     2401 cccttctcga ggctcgggtc tccgccgtcg agccgccgct cggcgacgtc gaacacctcg
     2461 acgtcgggag cgaccccttc gttctcgacg acccacttgc cgtcgtcgtc gtagatccgg
     2521 aaggtcggga cgtcgacgct gccgccgtcg acgaactcgg cgttcccgga gaggccgatc
     2581 aggcctcccc aggtgcgcgt cccgacgatc gggccgagct tctccttccg gaagaagtag
     2641 gggagggcgt cgccgccgga ggcggagtag gagttgacga gcatcgcctt cgggccgtcg
     2701 tgggcgaagc cgggcgttcg catggcgtcg acgccgcggc gggcccagaa cgcgagggtc
     2761 gtccgggaga gcatctcgat catccggtcg gggatgaagc cgccgccgtt gtatcggtcg
     2821 tcgacgagca gcgccggctt cgacgcctgg gagtagaaga gcttctggag catccggttc
     2881 ccggcgaggg ccgtgtcggg aaggtggatg tagccgacct tcccgcccga gagcttctcg
     2941 acgagcgcca tccggctctt cacccagtcg aggtagcgga gtccctgctc gctcgcgacg
     3001 gtccggacgg tcacggtccg gcccccctcc cgcgaggggg ccttcccgac gaggagcgtc
     3061 accgctcgcc cggccttccc ctcgaggagc cggtacgggt tgtcggccgt cgtcaggtcg
     3121 acgccgtcga tcgccaggag gagctcgcct tctgcgacgt tcacgccggg ctcggtgagc
     3181 ggcgagcgga acggctcgtc ccagttctcc ccggggaaga tccgggcgat ccggtagcgc
     3241 ccggaggggt cggccgtgag ctcgcagccg aggaagccgc cggggacccg ctcgaccttc
     3301 ggctcgtcgc ccgacgtgac gtaggtgtgc cccgcctcca gctcggagac gagctcgccg
     3361 aggaggaagt cgagatcggc ccggtgggcg acgaacggga cgagcgcacc gtagcgcgcc
     3421 acgagcgcgt tccagtcgac gccgtggagg ccggggtcgt agaaccagtc cctcacggtc
     3481 cgggctccgt cgcgccagat ctgggcccac tcggccggga ggtcgagctt cgtccgcagg
     3541 cccgaaaggt cgagcttgcc cgcgccggag gcgagcccgg cgcgggcgtc ggcgacgccg
     3601 agctccttgc cggacctcca gaggagcttc ttgccgtcgg ccgagaggcg gtaggccgag
     3661 acgccgtcga ggaccttctc ctccttccgc tccttgaggt cgaagcggaa gagggctccc
     3721 tcgtcctcac cgtcgcggac gtagtagacg gcctcctccg tcgcgcggag ggagcggagg
     3781 tcgccgggct tcacgcccgg gagggcgatc gtccgcgccg cgaaaccctc cgcgaggacg
     3841 acgacgcggg agggctcggc tgccttcccg ctcttcgcgt cgtccttctt cccgtccttc
     3901 ccgccgtcgg ccttgcccga ggcgggcttc gcggcggcct cggcctcgcc cgccgtcccc
     3961 ttttcctcgt cgctcttcag cgggaagaga tgagggacgt cgggggagag ggccgcggcg
     4021 tagacacgcg tcgccttcgg gtagacgtag gagaactcga aggcgctgaa ggtcggggcg
     4081 tagtcgcggt tcgagaggaa gaagaggtac ttcccgtccg ccgagaaggc gggggagaag
     4141 tcggccgtca ggccgtcgcc gaggcggatc ggcttcccgc cctcgagcgg ggcggcccag
     4201 agacccggca ggcgcgtcga gtggctcttc tcgtagacga gccagcggga gtcgggggag
     4261 aacgagtaga cgtcgatgtc ttcgaagccg ctccggtcga cctcggtcac cttcccgctc
     4321 gcgacgtcga cgacgcggag gcgttgggcg cgatcgccga aggcgatctt cttcccgtcg
     4381 gggctgaagg ccggcgcgtg cttccagggg gcgccgtcgg tcgtgaggcg gcggggcgtg
     4441 ccggtcccgt cctgcgggcg gatccagagg tcgtactcgc ccgtctcgtc ggagaggtag
     4501 gcgatccact tcccgtcggg cgaccaggcc gggtcgcgct cgcggacggc ggagccggtc
     4561 gtcagcggcc ggacgacgcc gtccttcgcc gggagggagt agaggtcgcc gcgggcgtcg
     4621 aggacgacgc gggcgccgct cggcgagagc gccatcccgg cgacgttccc ggcgacgtcc
     4681 cggaaggcgg ggaccgtggc cgccgcgtcc gagccgagcc ggatcgggat gcgcgcggcc
     4741 ttctcggtcg ccaggtcgag cgtccagagg tcgcccccgt tcatgaagac gatccgcccg
     4801 tcgccgaggc tcggccagag gacgtcgaac tcggtgaacg aggtgagctt ccgcgtctcc
     4861 ctcgtcttca ggtcgtagcg ccagaggttg agcgtccgct cgcggtccga ggtgaagtag
     4921 accgcgtccc cccaccacat cgggaagttg tccgtccctt cccacgtcgt gaggcgctcc
     4981 gagcgcttcg ccgcgaagtc gtagagccag acgtcctgcg cgcgcccgcc cttcgtcctc
     5041 ttccaggtcc ggaactcgcg atcgacgggg cagtaggcga ggcgtgcgcc gtcgggcgag
     5101 agcgaggccg agcctccctc ggggatctcg agcgccgtct cgaggccccc cttcgggtcg
     5161 acgaggaagt agcgccccat ccggccgttc caggggacgc ggttcatccg gacgaggacc
     5221 ttgccgtcct tcgtccagcc gaggacccag ttgtcccagc cgccgcgcgg cggcatcggg
     5281 ccgacgtccg tcgcgaacgt gagctgccgg ggcgtcccgc cgtgcgcggg gacgacccag
     5341 acctgccgcg tcccggtgta ctcggccgag aaggcgatcc acttcccgtc gtgcgagagc
     5401 ttcgggaaga gctcgaggcc ggggtgcgac gtgaggcgga acgcggcccc cccggagagc
     5461 gggacgcgcc agaggtcgcc tccgtagacg aagacgaccg tgtcgccgcg gacgtcgggg
     5521 aagcggagga ggcgggcctc cggagggccg tccgcgtcgg cgaggcccgg cggggccgac
     5581 aggaggagcg cggcgaacgt tgccgcgagg agcgggcgga ggagaggacg agagctcatg
     5641 acgcgattct gcccgacgcg cgacggtgtc ggccgtcacg cgcgcggcgc cttcccgcgc
     5701 tttcgctttg cccggttctg gtccatcatc cgggaaaatc gagaaacgga gcgaggcgaa
     5761 atcgatggct ttcctgaaag tcctggaatc gccggaccct gccctgaagg accagacgat
     5821 cccgctcgcc gggaaggaag tcctgatcgg ccgcggcaag gagaacggtc tcgtcctcga
     5881 ggaccacgcc gtgtcgcgcc accacgcccg gatcgtggag gaggcggagg ggcaccttct
     5941 ggtcgacaac aactccgcga acgggagctg ggtgaacggc cagcgcgtga cggaggcgag
     6001 gctgaagccg ggcgaccaga ttcgcctcgg caagacgctg ttcgagatcc agggggtcta
     6061 cgacccggag gggacggccc tcctggacgt ccagggaatg gggatgccga tgaagccgtc
     6121 ggcgccgccc gcggcgcccg cctctccgcc tcctcccgcg ccggctcctc cacaggtcgc
     6181 cgctcctccg ccggccccgc gccccgctcc tgtcgagacg ccgacggtgc cggaacccgt
     6241 cccgatgcgg cgcccgtctc cgccgccgac gccgatcccg ccgccgacgc ccgttccggc
     6301 gccgcgtccg gcgcctccgc cggcccccgt ggcggcgcgc ccggcgccgc cgcccgcgcc
     6361 gatggcggca cggccggcgc cgatggcgcc gcctcccgcg ccggccgcgg ctccggggta
     6421 ctacgacgag gcggccccgg tggaggggga gcccgccggc ttctggatcc ggttcctggc
     6481 gtcgctgatc gacagcgtga tcctcaacat cctgctggcg atcgtctggg cgccgacgtt
     6541 cttcatcacg ttccgcgccg cggccagcgg agaggctccg ggcgccctcg ccgcgatcct
     6601 gccggtcttc tcgttcctcc tcacgatggt cctctcgctc gggtacacgc tctggttctg
     6661 ggcgaacaag ggcgccaccc cgggcaagaa gatgctcggc ctgcgcatcg tccgcgagga
     6721 cggcgaggag ccgctcggct ggggcaccgc cttcatgcgc ctcgtcggct acatggtgag
     6781 cggcttcatc ctctacatcg gtttcttcat gatcgccttc aaccccgaga agcaggggct
     6841 ccacgacaag atcgcgaaga cccgggtgct gaaggtccgg tagccgggcc gaccgcctcc
     6901 ccgaacgtcg aaggccgggc ccgcgggccc ggccttcgtg cgtggtgggg agcgggacgg
     6961 ggcccgctcg gagtcagccc ttcgactcga gcttctcctt gaccaggaac gcgaccttca
     7021 ccccttcgaa gacgacccgc atgagcgaga ggtagacgac ggcgaggacc ggcgcgagga
     7081 cggtcatcac gaggccggtg acgatcacga agaaggagtc gaaccggaag ccggtgacga
     7141 ccgtcttcag gcccgagatg atgccgccga gcgccccgag ggcgcagagg gcggcgaaga
     7201 cgaggtagag gacccggatc agcttcgggg cgacgagggt ctcgaacttg aagtcgagga
     7261 gacccttgaa cgcgttcgcg tcgtccccga cgaggccgcc gatcgccgcg acggccgggg
     7321 agggccgctc cgcggcgggg tggcgcggcg cgggcgcggg ggccggagcg ggtgccnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgga gcgggcgccg gggcgggcgc
     7501 cgccgcgacg cggaacgacg ggagcgtctt cgggtcggtc cacgacccga gcgcctcgtt
     7561 ccagaccatc acctggcgcc cggcgtactg cgtgcagagc tgccggacct ggctcatgtc
     7621 gtgctcgccg tcgagctggc ggccgtccac ggccacgaag aagatctctg ggggcatctt
     7681 ctctcctttc agcgggcgaa gacttcggac accggggtga tccggactct ctccggcgtc
     7741 aggccgagct tccgggctcc gatgaccgcg tcctccgtga ggacctccgg gggtttcggg
     7801 gcatcgtacg cgatcgtgga gggctcggag gagagaagct gtttcttcag cccctcggcg
     7861 tccttcgtga tcacgacgac ggagagcgtc tcggccgaga ggtgcttccg gatcgcccgg
     7921 ttcacgtcgg cgcgggtcag cttcgcgagc ctctcgcgca ggtaggggac caggtcggcg
     7981 gtgccgtacc acctcgagtc gagggccgct ccgaggtcgt cgccctgggt cgaggtgagg
     8041 aggtagacgt tcttcatcag gtagtcgcgg gtccgctgga agtcggcctc ggagatcccc
     8101 ttgcggatca gccggtcgag ctcgtggagg gcgatcttca gcgccatcgg gccgttggcc
     8161 gggacgaccg ggcggatcca gacctcgaag agctgcgccc ttcgggcgac gttcggggcg
     8221 gggctcgtgg cgtacatgcc gtacgggaac gcctcgatgt aggcgtagtc gccgtagttc
     8281 atgccgcgca gctcgcggat cctctggtag aggtgcgcca tcgagttgcg gtgctcgccg
     8341 aggaacgcgc ggaagacgga gagggccgcg aagtcggggt gggagcgcgt cacctcgatc
     8401 gggaggccga aggagatcgc cgtggcgcgg gtctccttct gcacgatctc cacctcgagc
     8461 gcggggggcc ggcgcccctt gacgccggcg ggcgccggga gggccggccc ggcagcgagc
     8521 tcgccgaggc gggaaaggag gcgggccttc aggtcggccg ggacatcccc cgcgaggccg
     8581 accgtgagcg ccgcgcgcgt gtaggccgcc ttccagaagg ccttcacgtc gtcgagggcg
     8641 atcgcgtcga tcccgccgag cgtgccgagg ggcggatgcg cgtagggggt gccggcgaag
     8701 acgacggact ggaggcgctc cttgccgagc tcctcctcgt tgttcgcgat caggtcctgg
     8761 gcgagggcgt tgcgctgggc gtccttcagc cgccggaagt cctcctcgcg gaaccccggg
     8821 tcgaggagct gcgggagggc gacgtcgagg taggaggcga ggttgtcccg gtggacgctg
     8881 ccggtgaaga cggtcatctc ccggtcgacg ccggccgaga acgaggcggc aatcgggaag
     8941 agcgccttcc ggatctcgtc gatgccggcg tccttcgagc ccgcctccgc gaccatcgcg
     9001 gccgtcaggg ccgcgaggcc ttccttcccc ttcgggtcgt gcgcggagcc ggccgagaag
     9061 aggagcttga aggtcacctg cgggtgggac gacggccgct ccacgaccga gagcccctcg
     9121 ggggccgcga cggccggggc gagcgacccg agcgccggga gcttcgcgag cggctcgggc
     9181 aggggctcgt gcgagagcgt ggtctggacg aggccggcgt cgacgacgta cttcgcggcg
     9241 gcggcgcgga ggtcctcggg ggtcagcgcc tcgtagaggc ggtagagctc gttgagcgtg
     9301 ccgtactcgc ggcggtggcg gacgtactgc gcgagcgtcg aggcgatcgc ctcggtgttg
     9361 tcgaagctcc ggacgagcgc gtagcgggcg cgggactggg cgtcggcgag gcgccgggcg
     9421 ggaacgggct cggcccggag cgcggcgaac gtccggagga gctcgtcacg gacggcggcg
     9481 gcgtcctcga gcttcttgac gcgcgcggcg accatgagga gctgcgggtc ggcgctcgcg
     9541 gggaagtagg cgaagagctg gtcggccttc tgctcggtct cgacgagctt cttgtagacg
     9601 gccgaggtgg ggccgaagga gaggtcggag aggacctcga gcgccgccca gtccttctcg
     9661 gtggtggaga aggcggggcc gcggaaggag acgacgaccc aggggagggt cggcgtcggc
     9721 cacggcacgt gggcgctctt cggcccggag ggggccggct cccgcgggat ctcgaccgac
     9781 caggagccgc gcgtccagga gccccagtac ttctcgacga gcgggaggac cttcgccggg
     9841 tcgacgtcgc cggcgacgag gatcgtcgtg tactccgggc ggtaccaccg gtcgaagaac
     9901 tgcctcgagt aggcgaactg gttgggcatg tcctcgatgt cccggaggaa gcccatcgtc
     9961 gtgtgcttgt aggtgtgcgt cgtgaacgcc gcgtcgcgca tcgcctcgaa gagcttcgag
    10021 acggggttcg cgctgttctt gttgtactcg ccgaggacgg cgcgcgactc ggtcttgaac
    10081 gccgcctcgg ggtactcgag gttccggaag cggtcggcct cgatctcgag gaccttctcg
    10141 aggtcctcct tcgcgaacgt cgtgtggtag ttcgtgtagt cgtcggtcgt gtaggcgttc
    10201 tgggaggcgc cggcccgcgt caggatcgcc tggtactcct cgggcgggta ggacttcgtc
    10261 ccgcggaaca tcatgtgctc gaagaagtgg gcgaagcccg acttgcccgg ctccacctcg
    10321 ttccgcgacc cggtctgcac cgggatctgg agggagacga ggttcgggaa gccggtcggg
    10381 acgacgatca ccttcaggcc gttcggcagg gtcgtctcgg tcgccgggat cgtcaggacg
    10441 tctcgggaca tctcggcacc tctcggagcg gcgaagccgg gcgcggcggc gaggagggcg
    10501 gccgcgaggg cggcggaggc gggcagggcg gttcttctca tcggtctcct gggcggcgcg
    10561 ggaggcgccg cagctcggtc tgaggtcggg gaatcgccgt caggcgtccc gctcgtggag
    10621 gtaggcgcag tcgagggtcc tcacgccggc cgcgtcgccg acgacgtact tcgacccgct
    10681 ccagatcgcg gcgccggcgg tccgcccggc ggcgtcgacg cagtagaact ggacgttgaa
    10741 cgtggggcgt cccttcgcgt cccggtgccg cgggacgtgg tgggtcttct cggcgatccg
    10801 gcgaagggcc ttcaggcagg cctcctccgg ggcgaggccg tcgcgcaggt gctcgacgac
    10861 ggtgtgcgat ccgcaggtga ggaggttcgc ctcgccgcgc ccggtcgacc cggcggagcc
    10921 gacgccgttc tccgagtaga ggccggcgcc gatgacgggg gagtcgccga cgcggccggg
    10981 gatcttgaag aagaggcccg aggtcgtcgt cacgccgacg aggtcgcccg aggcggagcg
    11041 gacggacatg tggatcgttc cctgcgggcg gaagatcccc ttgccgtact tccggacgaa
    11101 ctccttcacg tcggggtcct cggcgtcctt cgcggcctcg atccagtcgt cgaggtcgga
    11161 gaggcggttc ttccagtaga gccagatctt ccgggtcttc tcggtaagga gctcctcgtc
    11221 ggggaagccg tgagcggtcg cgaaacggcg cgcgccggcg ccgacgagga agacgcggtc
    11281 ggtccgctcc atgacgagct tcgcgacctt cgaaggggtc ttcacgccgg tgagggcggc
    11341 gaccgcgccg gccttcatcg tcctcccgtc catgaccgcg gcgtccagct cgacgacccc
    11401 ctcctcgttc gggatgccgc cgtacccgac ggtgaggtcg gccgggtcga gctcgacgac
    11461 gttcacgccc gcgatcgcgg cgtcgagcgg gtcggtcccg gcgaggagcg cctcccgggc
    11521 ggccttcgcg gcctcgaggc cgttcccgga ggtgacgatc cggaatccgg tctccgccgg
    11581 gacgggctcg cgggcggcga gcccctt
//
LOCUS       DAOTEZ010000086        11542 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3924_length_11542_cov_5.342039, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000086 DAOTEZ010000000
VERSION     DAOTEZ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11542
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3924_length_11542_cov_5.342039"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="P5164_06410"
     CDS             <1..333
                     /locus_tag="P5164_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRY43564.1"
                     /translation="ERAAGVESNRLTRRLPFLATTASATPFIGLFGTVWGILGAFRQI
                     GVTGSTSIVTVAPGIAEALVNTAAGLVAAVPALIAYNYFNGRVKVIRSEMRDFALEFL
                     NLAERHFT"
     gene            353..775
                     /locus_tag="P5164_06415"
     CDS             353..775
                     /locus_tag="P5164_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HRY43565.1"
                     /translation="MAVKIDDDLEAYSDINVTPLVDVMLVLLIIFMVTAPLLHQGVEV
                     KLPKSVSQNLPKTPEDPLILSITRKEVYYLNETPIPKQQLRERLTLILKNRKDRAVYL
                     KADRNLPYGVVVETMDVLNRLGVESLGMVTELRSEKSP"
     gene            772..1533
                     /locus_tag="P5164_06420"
     CDS             772..1533
                     /locus_tag="P5164_06420"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRY43566.1"
                     /translation="MTVSEVLEERSRRPDAPHGLAAAFSLAAHAAFVVFLVLISQPRK
                     LTTPPLMSLPVRVVSPGALARLSGPPGRPAARPQDEAPRKRVIEKIRDEAPAPSAEAL
                     PLPGKPKKGEPPKPAPRASAPGPRPAASDAAVELPSAGNGGLGGSGSPGLSSFGASLS
                     GFDSDFPFSYYAEQIQALIGANWLKPEVPEGTACVVTFRIQRSGQVTEVKVETPSGLA
                     FYDRAAARAIYSANPLPPLPPEFRGEQLGVHIRFQ"
     gene            1554..2894
                     /locus_tag="P5164_06425"
     CDS             1554..2894
                     /locus_tag="P5164_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HRY43567.1"
                     /translation="MRLLSFATGLVLVAALAPVAGQQPVPTVAPSAQPTDISLVLSQE
                     GGRRVALAIPSPKAPASDVVEKDLVAPFRSALTGDLESSPWFVLADAALHPKGFRPPA
                     TREEGDAWIASGAQFLLDTQIQAEGTNVVVTAQLVDLRTLRPILGKRYSAAGVSIRRV
                     AHTIANDVVRQFTGRPGPFLTRIAFVSDRDGGRGKEIYMMDWDGENERRLTFHRSLSI
                     APDWSYDGSKIVYQSFVSTPPGLYWFSLSGGTRNRVPVPTELNSAPSFSPDGKMVAYA
                     GSVRGNPEIFVVNLDGSGHRRLTNSSAIDSSPRFSPNGREIAFTSNRQGTPQIYLMDL
                     EGANVRKLTLTGNWSDEPAFSPDGGRLAYACRNEGDFQICVLDLTTGRTFQISSGPGT
                     NENPTWSPDGSRIAWELQRGRSTQIVSAGVDGSGFKVLTEAGNNGYPVWERTME"
     gene            2992..3549
                     /gene="pal"
                     /locus_tag="P5164_06430"
     CDS             2992..3549
                     /gene="pal"
                     /locus_tag="P5164_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HRY43568.1"
                     /translation="MTKPFRSLGLVLAAGLLVTAGCCTKKPAPAPAPEVKVEPTPTPV
                     PVPVEEPIAAPVAEPVAESLPADLEELNKKGYLKDIFYDYDLYDVRADQRDAMAANAE
                     WLKKWPTVKVQVEGHCDERGSNKYNMALGDKRANAARDYLVSLGIDASRITTLSYGEE
                     RPFVEGQTEAAWSQNRRAHFVVTAK"
     gene            3546..4424
                     /gene="ybgF"
                     /locus_tag="P5164_06435"
     CDS             3546..4424
                     /gene="ybgF"
                     /locus_tag="P5164_06435"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HRY43569.1"
                     /translation="MTPLRVDRLRRSAPLAGALLFASVLGGCVSGSDIEGLHRHVSDV
                     EKQVDKLSQQTSSKDEVEKLNQNLTKQVSTILRSNAETGTRFDELTRELQSLAGKLED
                     SNRRLAQLSQQLAETQAKLDAGTPAAPAVPGAAPLPGASAPPPAGAAAAPGSIPATKP
                     PAGRAGPADLYNAALGDYQRGRFDLARQGFEEYVETYPRTDLSDDALFWAAECWTAQK
                     KHREALGMLERLLKEYPQSDKAPSAHLRKGLAHLELGEKAQAIVQLQFVVHEYPTSDE
                     AKSARQRLRSLGSDSR"
     gene            4488..4751
                     /gene="rpsT"
                     /locus_tag="P5164_06440"
     CDS             4488..4751
                     /gene="rpsT"
                     /locus_tag="P5164_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016443759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRY43570.1"
                     /translation="MANTKQAKKRAAQNDKQKARNRAVKSETRRVVKATRAAAPEKAK
                     EMLPKAAASLDVAARKGTIHKNKAARLKSRLAKGAAKKVAKAD"
     gene            complement(4871..5389)
                     /locus_tag="P5164_06445"
     CDS             complement(4871..5389)
                     /locus_tag="P5164_06445"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HRY43571.1"
                     /translation="MRRRAFLAAVLAAGLAGCGYSLVGTGRGILPEGASAIFVEAFVN
                     DTTRVGLEQRLTDAVLRELAGRARLSPVTDRSAADVELSGRILSYQVNPVRFDDQGRA
                     LEYEIAVVARLKLTDRKTEKALFENPSFLFRQPYPVAATSASYVDVENAAIEAMARPF
                     ARSLVTTILEGF"
     gene            complement(5386..7857)
                     /gene="leuS"
                     /locus_tag="P5164_06450"
     CDS             complement(5386..7857)
                     /gene="leuS"
                     /locus_tag="P5164_06450"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRY43572.1"
                     /translation="MDAREERTALEKKWQARWDADRAAFVDTHDPSAKGTYLLVMLPY
                     PSGDRLHVGHARTYYLTDALHRYLRQKGEKVFCPMGWDAFGLPAENFAIQRGIPPRES
                     TLANIAAMKEQFAGWGVLYDWTKEVTTCEPEYYRWNQWFFLRMLERGLAYRKKGTVNW
                     CPSCQTVLANEQAEGGTCERCKSAVEQRELEQWFLKITEYADSLLAGLDQLGKWSEKV
                     RTMQRNWIGRSVGADLEFEVPKLGERLQVFTTRPDTVYGATAMVLAPEHPAVPRLLEG
                     HPDRAALEEWVKTVRSQDRVQREAEGAEKDGRFTGAHAINPFTGEEMPIWMANFVLAG
                     YGTGAIMAVPGHDERDHEFAKKYGLPIREVIRRPDGADPSVACFTGEGTMTASGPYDG
                     LTSEAGREAIAAEATKRGIGGARVRYRLRDWLISRQRYWGTPIPMVKCPACGWVPVPD
                     DQLPVVLPKDAPFTGKGGNPLEKVEAFLKTSCPKCGGEARRETDTMDTFVDSSWYYLR
                     YLDPKNDRAPFDPEVIARWAPVNQYIGGIEHAILHLLYARFFARVMKDLGLVSFEEPF
                     SALFNQGMITKMSPGGRVEKMSKSRGNSVSLDPLIAAKGADAVRAYVLFLGPPESDAE
                     WSDDQIAGPERFLYRVKALVENYLKAHGTAPHALAAADSPAARKRHIAVKTVTHDFET
                     FSFHTAVAHLMEFTHHVAGLVGDAKADPGEMRASILALLALLHPIAPHLTEEWHEQMG
                     ETRSLLVSGWPAYDPALAVEESVTVAVQVGGKLRGQVVLPRGASREEALAAAEAQEGV
                     AKWLEGKERLKVVYVPDRLLNVVVK"
     gene            complement(7892..8956)
                     /locus_tag="P5164_06455"
     CDS             complement(7892..8956)
                     /locus_tag="P5164_06455"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HRY43573.1"
                     /translation="MRVLVVEDSAYQRLRIVEALRASSAITEVDAVGSGEEAIRRLLT
                     TDYALVTLDLGLPGMDGNAVLRWIMANRPVPVVVISADQEERSALAALEAGALEVIAK
                     AGSQPESLARWKRRLAEVVDGVRQLSIESLVRRSIRSGAPPSSGGIPRPRRVPVALGV
                     PPALVVAASTGGPPALRELFAGLPPQEAVVGVAQHMPPPFTRSLAHRLASGTGWDVRE
                     AIHGAEATPGVVWIAPGGAHLELSHAGERAVLVVRPHGGSVRWCPSADLLFTSAAAAF
                     GRRVVGVVLTGMGDDGAEGCRSIALAGGTVLCESAESAVISGMPDAAARAVPAARRFP
                     LSRLASEVERRLGEAAGAAA"
     gene            complement(8961..9785)
                     /locus_tag="P5164_06460"
     CDS             complement(8961..9785)
                     /locus_tag="P5164_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HRY43574.1"
                     /translation="MTGRPLELAETVFRLYQDLLRRRTGFVVSDSQRVRVESRLAAEA
                     RSAGSFYQLYGRLRNEPQDSPLFGRLLDAVVVGETYFFRDPASLKAFSEEIVPERLLA
                     AGQDGTLDIWSAGCATGEEAFSLSIVLHEKAILPARKVRILGTDLSPSHVRRARAAVY
                     GPQSLRATSPERRREHFEAAPGGLFRLKPQLAAPVLFETRSILDEPAFGTRFDVILCR
                     NVLIYLDEEARSRVVEVLSSRLKPGGYILLGASDALSVASSALTLVRLANDVVYRR"
     gene            complement(9782..>11542)
                     /locus_tag="P5164_06465"
     CDS             complement(9782..>11542)
                     /locus_tag="P5164_06465"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRY43575.1"
                     /translation="ALGRTGRREAIVPLLAALEEGNLWLQVHALEALGEVGDPEIAPR
                     ILPLLTNASLRHGALHALARLRSPVAGEPLLRRAAAGDVDVFLLAALRAALESASATT
                     LAGLRPLWPEAGATLLALLEDPEEDAALRTDAAHVLALLDLPGASLAIVSHGPFRDGY
                     AALRALPRARREEALLCVLQVADPEPALELLDAHRGGPEAPTLLPLLVHPSATVKAAV
                     LSCVPAGLVPIPDLIDVLAEDDPETALAAALALATDESGEPLDRLPKRRRALLDRAGG
                     PDGPGRVAALAALGLADGVDVEAAVRGALSSPDPAVREAAVAAAARGRRIGAHEIVTC
                     LSDESATVRAAALRGLALRAERGETLPGVTWRDGLVFLVDDPAAAAAAGAAVVALAGA
                     ERPRLVEEMLVQEPAVRIAALEEIERTGDAEAARSAVAALGHEDPETACAAVRALAVA
                     DEPVAAEALPTALADERAEVRAAAAETIARRPAGDSRSALSAAVATALVTEPDRAVLR
                     SLLVAASRIADETMVEPLTLVLSQETVPAEADEAAEALARLSPEACRRAWMHAPARAE
                     RRWARALAAAARAERSQRST"
BASE COUNT         1708 a         4220 c         4060 g         1554 t
ORIGIN      
        1 gagcgggccg cgggcgtgga gtccaacagg ctcacccgca ggctcccgtt cctggcgacg
       61 acggcctcgg cgaccccctt catcgggctc ttcggaacgg tgtgggggat tctcggggcg
      121 ttccggcaga tcggcgtcac gggctcgacc tcgatcgtca ccgtcgcccc cgggatcgcc
      181 gaggcgctgg tcaacaccgc cgccggcctc gtcgccgcgg tcccggcgct catcgcctac
      241 aactacttca acggccgcgt gaaggtgatc cgttccgaga tgcgggactt cgcgctcgag
      301 ttcctcaacc tggccgagcg ccacttcacc tgaggcgccg caaggagcgc ggatggccgt
      361 aaagatcgac gacgacctcg aggcctactc cgacatcaac gtcacccccc tggtcgacgt
      421 gatgctggtc ctgctcatca tcttcatggt gacggcgccg ctcctccacc agggcgtcga
      481 ggtgaagctt ccgaagtcgg tctcgcagaa ccttccgaag actcccgagg accccctcat
      541 cctctcgatc acgaggaagg aggtctatta cctcaacgag acgccgatcc cgaagcagca
      601 gctgcgcgag aggctgaccc tcatcctgaa gaaccggaag gaccgggcgg tctacctcaa
      661 ggccgaccgg aacctgccgt acggcgtcgt cgtcgagacg atggacgtcc tgaaccgcct
      721 cggggtcgag agcctcggga tggtcacgga gctccggagc gagaagagcc cgtgacggtc
      781 tcggaggtcc tggaagagcg gagccggcgg cccgacgcgc cgcacggact cgcggccgcg
      841 ttctcgctcg cggcgcacgc ggcgttcgtc gtcttcctgg tcctcatctc ccagccccgg
      901 aagctcacga cgcctcctct gatgtccctt ccggtccgag tcgtctcgcc cggcgccctc
      961 gcacggctct ccggtccgcc cggacgcccc gcggcccgtc cgcaggacga ggcgccccga
     1021 aagagagtca tcgagaagat ccgcgacgag gccccggccc cgtccgccga ggctctccct
     1081 ctccccggca agccgaagaa gggggagccg ccgaagccgg cgccgcgcgc atctgccccc
     1141 gggcccaggc cggccgcgag cgacgccgcg gtcgagctcc cctccgcggg gaacgggggc
     1201 ctcggaggga gcggcagccc cggcctctcg agcttcggtg cctcactgtc cggcttcgac
     1261 tccgacttcc ccttctccta ttacgccgag cagatccagg ccctcatcgg cgccaactgg
     1321 ctgaagcccg aggtcccgga ggggaccgcc tgcgtcgtca cgttccgcat ccagcgctcc
     1381 ggccaggtca ccgaggtgaa ggtcgagacc ccctcaggcc tcgcctttta cgatcgtgcc
     1441 gccgcgcgcg ccatctactc cgccaacccg ctcccgccgc tcccgcccga gttccggggc
     1501 gagcagctgg gggtccacat ccgcttccag tgaagccccc ccggaggtcc gcgatgcgtc
     1561 tcctctcctt cgccacgggc ctcgtgctcg tcgccgccct cgccccggtc gccgggcagc
     1621 agccggtccc gacggtcgcg ccgtcggcgc agccgaccga catttccctc gtcctctcgc
     1681 aggagggcgg acgccgggtc gcgctcgcga tcccgtcccc caaggcgccg gcctccgacg
     1741 tcgtcgagaa ggacctggtc gcccccttcc gctcggccct gacgggtgac ctggagagct
     1801 ccccctggtt cgtcctcgcg gacgccgccc tccacccgaa gggcttccgc ccgccggcaa
     1861 cgcgggaaga gggcgatgcc tggatcgcct cgggcgcgca gttcctcctc gacacgcaga
     1921 tccaggccga ggggacgaac gtcgtcgtca cggcccagct cgtcgacctg cggacgctcc
     1981 gtccgatcct cggaaagagg tactcggccg cgggcgtctc gatccgccgg gtggcgcaca
     2041 ccatcgccaa cgacgtcgtg cgccagttca cggggcgtcc cgggccgttc ctgacgcgga
     2101 tcgccttcgt ctccgaccgg gacggcggac gcggcaagga gatctacatg atggactggg
     2161 acggcgagaa cgagaggcgg ctcacgttcc accgctctct gtcgatcgcg cccgactggt
     2221 cctacgacgg ctcgaagatc gtctaccagt cgttcgtgag cacccccccg ggcctctact
     2281 ggttctccct ctcgggcggg acgcggaacc gggtcccggt gccgacagag ctgaactcgg
     2341 cgccgtcgtt ctctccggac gggaagatgg tcgcctacgc cgggagcgtc cgcgggaacc
     2401 cggagatctt cgtcgtgaac ctcgacggct cgggtcatcg gaggctgacg aactcgtcgg
     2461 cgatcgactc cagcccccgc ttctccccga acggaaggga gatcgccttc acttcgaacc
     2521 ggcaggggac gccccagatc tacctcatgg acctcgaagg ggcgaacgtc aggaagctga
     2581 ccctgaccgg caactggagc gacgagcccg ccttctcccc cgacggtggc cgtctcgcct
     2641 acgcctgccg aaacgagggg gacttccaga tctgcgtcct cgacctgacg acgggacgca
     2701 ccttccagat ctcgagcgga cccgggacga acgagaaccc cacctggtct ccggacggct
     2761 cccggatcgc ctgggagctc cagcgcggcc gctcgaccca gatcgtctcg gccggggtgg
     2821 acggctccgg cttcaaggtc ctgacggagg ccgggaataa cggctatcct gtctgggaac
     2881 ggacgatgga gtgatcccgc tggccgggtt gtccgagccg gcggcagcgc ttagaatcga
     2941 gagtaactga aagggcggat cgcgcctttt cccaatcgga ggttcaccgt gatgacgaag
     3001 ccgttcagat ctctgggact ggttctcgcc gccggccttc tcgtgaccgc gggctgctgc
     3061 acgaagaagc ccgccccggc cccggccccg gaagtgaagg tcgaaccgac tccgaccccc
     3121 gtccccgtcc cggtcgagga gccgatcgcc gcgcccgtcg cggaaccggt ggccgaatcg
     3181 cttcccgccg accttgagga gctcaacaag aagggctacc tcaaggacat cttctacgac
     3241 tacgacctct acgacgtccg ggccgatcag cgggacgcca tggccgcgaa cgccgagtgg
     3301 ctgaagaagt ggcccaccgt gaaggtccag gtcgagggcc actgcgacga gcggggctcc
     3361 aacaagtaca acatggccct gggcgacaag cgcgcgaacg ccgcgcgcga ctacctcgtc
     3421 tcgctcggca tcgacgcgag ccggatcacg acgctctcct acggcgagga aaggcccttc
     3481 gtcgagggcc agacggaggc cgcctggtcc cagaaccggc gcgcccactt cgtcgtcacc
     3541 gcgaagtgac gccgctccgc gtcgaccgtc tccgccggag cgcccccctc gcgggggcgc
     3601 tcctcttcgc gtccgtcctg ggcggctgcg tctccgggag cgacatcgaa gggctccacc
     3661 gacacgtttc cgacgtcgag aagcaggtcg acaagctctc ccagcagacg agctcgaagg
     3721 acgaggtcga gaagctcaac cagaacctga cgaagcaggt gtccacgatc ctgcgctcga
     3781 acgccgagac cgggacccgc ttcgacgagc tgacccggga gctccagtcg ctcgccggca
     3841 agctggagga ctccaaccgc cggctcgcgc agctctccca gcagctcgcc gagacccagg
     3901 cgaagctgga cgccggcacg cccgcggccc ccgccgtgcc gggcgccgct ccccttcccg
     3961 gcgcgtcggc gcctccgcct gccggcgccg ccgcggcccc gggctcgatc ccggcgacga
     4021 agcctccggc cgggcgggcc ggcccggccg acctctacaa tgccgcgctc ggcgactacc
     4081 agcggggacg attcgatctg gcccggcagg gcttcgagga gtacgtcgag acgtaccctc
     4141 ggaccgacct ctccgacgac gccctcttct gggcggccga gtgctggacg gcgcagaaga
     4201 agcaccgcga agcgctcggc atgctcgagc ggctcctgaa ggaatacccg cagagcgaca
     4261 aggccccctc ggcgcacctg cggaaggggc tcgcccacct ggagctcggc gagaaggcgc
     4321 aggccatcgt ccagctccag ttcgtcgtcc acgagtaccc gacctcggac gaggccaaga
     4381 gcgcgcgcca gcggctgcgc tccctcggct ccgactcccg ctgaaccctc gataatcctc
     4441 cgcccgggcg gagggaagaa gacccgggac cgacccgaaa ggaaacgatg gccaacacga
     4501 agcaggcgaa gaagcgtgcc gctcagaacg acaagcagaa ggcgcggaac cgcgcggtga
     4561 agagcgagac ccgacgcgtg gtcaaggcga cccgcgccgc ggctcccgag aaggcgaagg
     4621 agatgctgcc gaaggccgcc gcctccctcg acgtcgccgc ccggaagggg acgattcaca
     4681 agaacaaggc cgcgcggctg aagtcgcggc tcgccaaggg cgccgccaag aaggtcgcca
     4741 aggcggactg accgagctcc gcgcgtcgcg cggagcccac gagccgggag ggccggagac
     4801 gaaccccggg gggttcgcgt ccggcgcccg gctctttccg tctcccggcc cgtcccgcgg
     4861 ccggcgggcg tcagaagcct tcgaggatcg tcgtgacgag actgcgcgcg aaggggcgcg
     4921 ccatcgcctc gatcgccgcg ttctccacgt cgacgtagct cgcgctcgtc gccgcgacgg
     4981 gatacggctg gcggaagagg aacgacgggt tctcgaagag cgccttctcc gtcttccggt
     5041 cggtcagctt caggcgggcc acgaccgcga tctcgtactc gagcgcgcgt ccctggtcgt
     5101 cgaaccggac ggggttcacc tggtacgaca ggatccgccc cgagagctcg acgtcggccg
     5161 cggagcggtc cgtcacgggg gacagccggg cgcggcccgc cagctcgcgg aggacggcgt
     5221 cggtgaggcg ctgctcgagc ccgacgcgcg tcgtgtcgtt cacgaaggcc tccacgaaga
     5281 tggcgctcgc tccctccggg aggatgccgc gtcccgttcc gacgagggag tagccgcaac
     5341 ccgcgagccc ggccgcaagg accgcggcga ggaacgcccg gcgcctcact tcaccacgac
     5401 gttcaggagc cggtccggca cgtagacgac cttcagccgc tccttcccct cgagccactt
     5461 cgcgacgccc tcctgggcct cggcggcggc gagcgcctcc tcgcgcgagg cgccgcgcgg
     5521 caggacgacc tgcccgcgca gcttgccgcc gacctggacg gcgaccgtca ccgactcctc
     5581 cacggcgagc gccgggtcgt acgccggcca gcccgacacc aggaggctcc tcgtctcccc
     5641 catctgctcg tgccactcct cggtcaggtg cggcgcgatc gggtggagca gcgcgaggag
     5701 cgcgaggatc gaggcgcgca tctcgcccgg atcggccttc gcgtcgccga cgagcccggc
     5761 gacgtggtgg gtgaactcca tcaggtgggc cacggccgtg tggaacgaga acgtctcgaa
     5821 atcgtgcgtc accgtcttca cggcgatgtg acgcttccgg gccgcgggcg agtcggcggc
     5881 ggccagggcg tgcggcgccg ttccgtgcgc cttcaggtag ttctcgacga gcgccttcac
     5941 ccggtagagg aatcgctccg ggccggcgat ctgatcgtcg ctccactcgg cgtccgactc
     6001 gggcggcccg aggaagagga cgtaggcgcg gacggcgtcg gcccccttcg ccgcgatgag
     6061 cgggtcgagc gagaccgagt tcccgcggga cttcgacatc ttctcgacgc gcccgccggg
     6121 cgacatcttc gtgatcattc cctggttgaa gagggccgag aacggctcct cgaaggaaac
     6181 gagcccgagg tccttcatca cgcgcgcgaa gaagcgggcg tagaggaggt ggaggatcgc
     6241 gtgctcgatc ccgccgatgt actggttcac cggcgcccag cgggcgatca cctccgggtc
     6301 gaacggggcg cggtcgttct tcgggtcgag gtaccggagg tagtaccagg acgaatcgac
     6361 gaacgtgtcc atcgtgtccg tttcgcgccg ggcctcgccg ccgcacttcg gacaggaggt
     6421 cttcaggaac gcctcgacct tctcgagcgg gttgcccccc ttgccggtga aaggcgcgtc
     6481 cttcgggagg acgacgggga gctggtcgtc gggcacgggg acccagccgc aggccgggca
     6541 cttcaccatc gggatcggcg tcccccagta gcgctggcgc gagatgagcc agtcgcggag
     6601 ccggtagcgg acgcgggccc cgccgatgcc gcgcttcgtc gcctcggccg cgatcgcctc
     6661 ccgtcccgct tccgacgtca gtccgtcgta ggggccggaa gccgtcatgg tcccctcgcc
     6721 cgtgaagcag gcgaccgacg ggtcggcccc gtcgggccgc cggatcacct cgcggatggg
     6781 gagcccgtat ttcttcgcga actcgtggtc gcgctcgtcg tggcccggaa cggccatgat
     6841 cgcgcccgtg ccgtagccgg cgaggacgaa gttcgccatc cagatcggca tctcctcgcc
     6901 ggtgaacggg ttgatggcat gcgccccggt gaagcgcccg tccttctcgg ccccttcggc
     6961 ctcgcgctgg acgcggtcct ggctccggac cgtcttcacc cactcctcga gcgcggccct
     7021 gtcgggatgc ccctcgagga ggcgcggcac ggccgggtgc tcgggggcga ggaccatcgc
     7081 cgtcgcgccg tagacggtgt cgggccgggt cgtgaagacc tggagcctct cgccgagctt
     7141 cggcacctcg aactcgaggt cggcgccgac ggagcggccg atccagttcc tctgcatcgt
     7201 ccggaccttc tcggaccact tcccgagctg gtcgaggccg gcgaggaggc tgtcggcgta
     7261 ctccgtgatc ttcaggaacc actgctcgag ctcgcgctgc tcgacggcgc tcttgcagcg
     7321 ttcgcacgtg ccgccctcgg cctgctcgtt cgcgaggacc gtctggcagg aggggcacca
     7381 gttgacggtc cccttcttgc ggtacgcgag ccctctctcg agcatccgga ggaagaacca
     7441 ctggttccag cggtagtact ccggctcgca cgtcgtgact tccttcgtcc agtcgtagag
     7501 gacgccccac cccgcgaact gctccttcat cgccgcgatg ttcgcgagcg tcgattcgcg
     7561 cggcgggata ccgcgctgga tcgcgaagtt ctccgcgggg aggccgaagg cgtcccaccc
     7621 catcgggcag aagaccttct cccctttctg ccggaggtat cggtggagcg cgtccgtcag
     7681 gtagtaggtt cgcgcgtgcc cgacgtggag acggtccccc gacgggtacg ggagcatgac
     7741 gaggaggtag gtccccttcg ccgacgggtc gtgcgtgtcg acgaaggcgg ctcggtcggc
     7801 gtcccagcgg gcctgccact tcttctcgag ggcggtgcgt tcttcgcgtg cgtccataag
     7861 ggacgccgag tgtagcaacc gggcgggccg gctacgcggc ggcgcccgcc gcctcgccga
     7921 gccgccgctc gacctcggag gcgaggcggg agagggggaa cctgcgggcc gcggggaccg
     7981 cgcgggcggc ggcgtcgggc attcccgata tgacggccga ctccgccgac tcgcagagga
     8041 ccgttccccc ggcgagcgcg atcgaccggc agccctccgc tccgtcgtcg cccatccccg
     8101 tcaggaccac gccgacgacg cggcgcccga aggccgcggc ggcggacgtg aagaggaggt
     8161 cggcggacgg gcaccaccgg accgagcctc cgtggggccg gacgacgagg acggcgcgct
     8221 ccccggcgtg ggagagctcc aggtgcgccc ctccgggggc gatccagacg actccgggag
     8281 tcgcctccgc gccgtggatc gcttcgcgca cgtcccaccc cgtcccggac gcgagccgat
     8341 gggcgaggga gcgggtgaag gggggcggca tgtgctgtgc gacgccgacg accgcctcct
     8401 ggggcggaag gcccgcgaag agctctcgaa gcgccggcgg tcccccggtc gaggcggcga
     8461 cgacgagcgc cggggggacg ccgagcgcga ccggcacgcg ccgaggccgg gggatgccgc
     8521 cgctcgaggg aggcgccccg gagcggatcg agcgtcgcac caggctctcg atcgacagct
     8581 gccggacgcc gtcgacgacc tccgcgagcc gtctcttcca gcgcgccagg ctctccggct
     8641 gcgaccccgc tttcgcgatg acctcgaggg cccccgcctc cagcgcggcg agggcgctcc
     8701 gctcctcctg atcggccgag atgacgacga cgggcaccgg ccggttcgcc atgatccacc
     8761 ggaggaccgc gttgccgtcc attccgggca gtccgaggtc cagcgtgacg agcgcgtagt
     8821 ccgtcgtcag gagccggcgg atcgcctctt ccccgcttcc gaccgcatcg acctctgtga
     8881 tcgcgctcga ggcgcggagc gcctcgacga tccggagcct ctggtaggcg gagtcctcga
     8941 cgaccaggac cctcacgccg tcacctccgg tagacgacgt cgttcgcgag gcggacgagc
     9001 gtcagcgccg aggaggcgac ggaaagggcg tcgctcgcgc cgaggaggat gtagcccccg
     9061 ggcttgaggc gcgacgagag aacctcgacg acccgggatc gcgcctcctc gtcgaggtag
     9121 atgaggacgt tgcggcagag gatgacgtcg aagcgggtgc cgaaggccgg ctcgtccagg
     9181 atcgagcgcg tctcgaagag gacgggagcc gcgagctgag gcttcagccg gaagagcccg
     9241 ccgggggcgg cctcgaaatg ctcgcggcgc ctctccgggg acgtcgcgcg gagcgactgg
     9301 gggccgtaca cggcggcccg cgctcggcgc acgtgagacg gggaaagatc cgtcccgagg
     9361 atccggacct tccgggcggg aaggatcgcc ttctcgtgaa ggacgatcga gaggctgaag
     9421 gcctcctcgc ccgtcgcgca gccagcggac cagatgtcga gcgtcccgtc ctggccggcc
     9481 gccaggagcc tctcgggaac gatctcctcc gagaacgcct tcaggctggc cgggtcccgg
     9541 aagaagtaag tctccccgac gacgaccgcg tcgaggagcc ggccgaagag gggcgaatcc
     9601 tgcggctcgt tccgaagccg gccgtagagc tggtagaacg accccgcgct cctggcctct
     9661 gccgcgagcc ggctctcgac ccggactctc tgggagtcgg agacgacgaa tcccgtgcga
     9721 cgccgcagga ggtcctggta gaggcgaaag accgtctcgg ccagctccag cgggcgccct
     9781 gtcacgtcga cctctggctt cgttccgccc gggccgccgc ggccagggcg cgcgcccagc
     9841 gcctctccgc gcgggccggc gcatgcatcc aggcccggcg gcaggcctcc ggggagagcc
     9901 gcgcgagggc ttccgccgcc tcgtccgcct ccgcgggcac cgtctcctgc gagaggacga
     9961 gcgtcagcgg ctcgaccatc gtctcgtccg cgatgcggga cgcggccacg aggagcgacc
    10021 tgaggaccgc gcggtccggc tcggtgacga gggccgtcgc gactgctgcc gagagggcgg
    10081 aacgcgagtc cccggccggc cgacgggcga tcgtctcggc cgcggccgct ctcacctccg
    10141 cgcgctcgtc ggcgagggcc gtgggcagag cctcggccgc gacgggctcg tcggcgacgg
    10201 cgagagcgcg caccgccgcg caggccgtct ccgggtcctc gtgcccgagc gcggcgacgg
    10261 cgctccgggc cgcctccgcg tcgccggtcc gctcgatctc ctcgagcgcg gcgatccgga
    10321 cggccggctc ctggacgagc atctcctcca cgaggcgggg acgctcggcg ccggccagcg
    10381 cgaccacggc cgcgccggcg gccgcggcgg ccgccgggtc gtcgaccagg aagaccaggc
    10441 cgtctctcca ggtgacgccg ggcagcgtct cgccgcgctc ggcccggagg gcgaggcctc
    10501 gaagcgccgc agctctcacg gtcgcgctct cgtcggaaag gcacgtcacg atctcgtgcg
    10561 ctccgatccg gcgtccccgc gcggccgcgg ccaccgccgc ttcccgcacg gcgggatcgg
    10621 gcgacgacag cgcaccgcga acggccgcct ccacgtccac gccgtcggcc aagccgagcg
    10681 cggcgagcgc ggcgacgcgg cccgggccgt ccgggccgcc cgcccggtcg agaagggccc
    10741 gccgcctctt cgggagccgg tcgagcggct ctcccgactc gtcggtcgcc agcgccagcg
    10801 cggcggcgag agccgtctcg gggtcgtcct cggcgaggac gtcgatcagg tctggaatcg
    10861 ggaccagccc cgccggcacg cacgagagga ccgccgcctt caccgtcgcg gacgggtgga
    10921 cgaggagcgg caggagcgtc ggcgcctccg gcccgccgcg atgcgcgtcg aggagctcga
    10981 gagcgggctc ggggtccgcc acctggagga cgcagaggag cgcctcctcc cggcgggcac
    11041 ggggaagcgc ccggagggcg gcgtagccgt cgcggaacgg tccgtgggag acgatcgcga
    11101 gggaggcgcc cgggaggtcg aggagggcga ggacgtgcgc ggcgtcggtc cggagcgcgg
    11161 cgtcctcctc cggatcctcg aggagcgcga ggagtgtggc gccggcctcg ggccagagcg
    11221 ggcggaggcc ggcgagcgtc gtcgccgagg cggactcgag cgcggcacgc agcgccgcca
    11281 ggagaaacac gtcgacgtcc cccgcagcgg ctcgccggag gagaggctcg ccggcgacgg
    11341 gagaccgaag gcgggcgagg gcgtgcaggg ccccgtggcg caggctcgca ttcgtcagca
    11401 gcggcaggat gcgcggcgcg atctcggggt cgccgacctc gccgagggcc tccagcgcat
    11461 ggacctggag ccagaggttc ccttcctcga gcgccgcgag cagcggcacg atcgcttctc
    11521 tccggcccgt ccggccgagc gc
//
LOCUS       DAOTEZ010000087        11532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3933_length_11532_cov_6.488542, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000087 DAOTEZ010000000
VERSION     DAOTEZ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11532
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3933_length_11532_cov_6.488542"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="P5164_06470"
     CDS             complement(<1..261)
                     /locus_tag="P5164_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HRY43576.1"
                     /translation="MTATRREFIQIGGAGLGAAALGSGLTTKWWGLDPDVVHDPGTDG
                     DQVVPTFCELCFWKCGVLAHVKDGKVTKLVGNPEHPLSRGKLC"
     gene            521..1756
                     /locus_tag="P5164_06475"
     CDS             521..1756
                     /locus_tag="P5164_06475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRY43577.1"
                     /translation="MIRSSRTPVWIVALAVVFASALLGTAGLASRTLRQRDAAVRDGV
                     LSRAAHELEASLRGATPEEASTVLQEFLSRYGASVDGAAIVGPSGVLASAGVDSTDGY
                     EVTAALGREWRPALLGAEPGSGAGFMGGRGPGPGAGRGMPPIRLRLRPSPALGRAPAL
                     AGLVLAGGAAAALALVGLALVAARGLAERARREAAEAEGQRLATVARAGAGLAHRLRN
                     PLAVVKGTAQLLDGRVPESERERVVRIVTASERMETILSRLLDFARPPDPQPVSFDLA
                     ALAREVASRGGEGTAAAVAVRADRPVPVRADREHVETILEELLANARAFDPNGTLEVT
                     VRAEGNRAVVEVADRGPGLDLDPASAFEPYVTSRPDGTGLGLAIVRSLATANGGAASL
                     APRSGGGCVATLALPGEGA"
     gene            1758..3032
                     /locus_tag="P5164_06480"
     CDS             1758..3032
                     /locus_tag="P5164_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRY43578.1"
                     /translation="MARLLVVDDEPSFRALLRDILEGAGHGVTEARDGAEALAFLERT
                     SFDLVLTDRRMPVVDGLELLRRVRARTSPPPVVVLTAHGSIPEAVEAVRLGAADYITK
                     PLPSPEALVDLVASLQPREDDGELVTADPAMKELLELVDRVAPRDVAVLVTGESGTGK
                     ELVARRLHARSPRAKGPFVAVNAAALPETLAESELFGAERGAFTGADQARAGRFEEAS
                     GGTLFLDEVGELPVGLQSKLLRVLEERVVRRLGGSRDLPVDVRLVAATNRDLTRETEE
                     GGFRQDLFFRLAVVVVNVPPLRERPDDVPLLARHFAQRLAARHGVPVPSLADDALAAL
                     SAHAWPGNVRELRNVLERAVVVRGGEPIRGADLGLAPASSAFAPLDRAHREREALLAA
                     LRRTGGKREEAAKLLGISVRTLYYRLKQWGMG"
     gene            3259..4131
                     /locus_tag="P5164_06485"
     CDS             3259..4131
                     /locus_tag="P5164_06485"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HRY43579.1"
                     /translation="MKKTALLAALLLATPLLAQPGPRGGQGNGPGAGAGAPAAPAASP
                     FVTYFASLPKEPLGSAEAAEIAFLREEEKLARDVYRALFAAHGDRAFANIAAAEQRHM
                     DLVKLLLDRYELADPAAATAPGEFEDARLAALYTDLVAKGKVSLVEALKVGATIEDLD
                     LADLAKALAASDNRDVDAVMQNLAKGSRNHLRAFAGRLQALGAPYAARYLPADDVAAI
                     LAAPRERGAVDEIGKLLPGTGPGLGRMGRGRGPGAGAGNGTGTCDGTGPGAGTGPGAG
                     FGPGDGTGAGCPNR"
     gene            4258..5850
                     /locus_tag="P5164_06490"
     CDS             4258..5850
                     /locus_tag="P5164_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter"
                     /protein_id="HRY43580.1"
                     /translation="MPSDKALLLTAMGAYLALTIGVGLWYSRRSARSAEAYFLGERGL
                     GPWVAAMSAEASDMSGWLLMGLPGVAYFAGAGEAAWTAIGLALGTFACWSLVARRLRA
                     HSIVAGNAFTLPAFFSARFHDDRRLLSTIAAGMLLFFFSIYVGAQFVTFGKLFGYVLG
                     MEGFYARMVVFGALFVLAYTFIGGFLAETMSDFIQGTLMFCALVLVLSVGTAKAGGVG
                     ALLAELGAIPRFTDLFGVATPLDAAGAVVTAAAGNAQALVGGVPAFGPGVGYGFLAIL
                     SCLAWGLGYFGMPQVLLRFMAIEKPAMVARARAIAVTWCVVSLLAAVGIGLIGRALMP
                     ARLLTASAAETIFIHLAVDFFPPLLAGLVLSGIVAASISSADSYMLIASSSLANDLVP
                     NFRAEPLSDRSILGLARGTQVAVTAFGILVALSGNDSIFRVVSYAWAGLGAAFGPLVL
                     FSLFWRRTTLAGAVAGMLTGGIAVVAWKNLLAPLGGAFAVYELLPAFLLSSLAIAVVS
                     RLTPAPPAEVTDAFDEARTFPG"
     gene            complement(5860..7032)
                     /locus_tag="P5164_06495"
     CDS             complement(5860..7032)
                     /locus_tag="P5164_06495"
                     /inference="COORDINATES: protein motif:HMM:NF027708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sb-PDE family phosphodiesterase"
                     /protein_id="HRY43581.1"
                     /translation="MPRRSAPLRLAGLAVLATVAALARTAPAHEVRVRRPLRVPDIPG
                     FVTLRCDFHTHTVFSDGSVWPDIRSEEAWREGYDAIAITDHIEYQPHEADLPTSHDRP
                     FEIAAPLGEKLGVLVVRGSEVTRKMPPGHFNAIFLKTSKPLETVEWRDALRAARAQGA
                     FLFWNHPGWRGQQKDGVARWYPEHDEILAAGLMDGIEVANDREYYPEAHGWALEKDLA
                     ILANSDIHDPSNLAWHVHEGDRRPVTLVFAKERSEEGIREALFARRTAAWADGKLAGR
                     EKELRPLVAAAVTIENPKLAVRAGDEGYVRLRNDSDLRLVLEGEGAAGEVVVPGKLVV
                     AANGTSLLEVRVPKRTAPGPRRLELVYAVSNVRPTPDAGLPFPVAIELEVLPPAPK"
     gene            7125..8036
                     /locus_tag="P5164_06500"
                     /pseudo
     CDS             7125..8036
                     /locus_tag="P5164_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            8072..8596
                     /locus_tag="P5164_06505"
     CDS             8072..8596
                     /locus_tag="P5164_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HRY43582.1"
                     /translation="MRDERDLDAAFAQRAPYLFHGAEGERSPDQGKRSFQCSRRLDAL
                     KVWVALQRYGADGLGALYDGLCSGARTLHAALAARRDFETLHEPESNILCFRWVGGGE
                     LDAERLDGINLRLREAFNRSGEGWITTTLLGGRRVLRATQMNPRTTAADLERVVEGLA
                     RTGREIAARSADRP"
     gene            8697..9101
                     /locus_tag="P5164_06510"
     CDS             8697..9101
                     /locus_tag="P5164_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HRY43583.1"
                     /translation="MNLCIPVSADRGLESPVSGHFGSAPLYLLVDSETRATRTLTNAR
                     AVHEHGACRPLDALAGERVDALVVGGIGAGALAKLRGAGIRVFRATAPTASACLDAFL
                     RNEVEEIDPAGACAGHEHDHGAGHAAGLPTRT"
     gene            complement(9147..10175)
                     /gene="cydB"
                     /locus_tag="P5164_06515"
     CDS             complement(9147..10175)
                     /gene="cydB"
                     /locus_tag="P5164_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HRY43584.1"
                     /translation="MSFEALAITWFVLEGVLLCGYAILDGFDLGVGILHPFVPRTDLE
                     RRISMNSIGPLWDGNEVWLVTFGGALFAAFPEGYATVFSGFYTAFMLLLVALISRAVS
                     MEFRSKMASPAWRRFWDWAFFGGSFTATLLFGVAVGNAMLGVPIDANGNVTGTVVDQL
                     GPYPLLVGAMTVALFAMHGGLFLYLKTEGEFQQRLREWMWRSWGLFMVFFMLTTVATV
                     AFIPRATENFRSFPWAIVVVVGTILAVANIPRCLYRDRPGLAFLSSAGVIAGLVALFG
                     LALYPNLVTAAGDPSRSVTVFNAASSPKTLGIMLVIAAVGMPFVLTYSGVIYWTFRGK
                     VKLSEHSY"
     gene            complement(10186..>11532)
                     /locus_tag="P5164_06520"
     CDS             complement(10186..>11532)
                     /locus_tag="P5164_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013627922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HRY43585.1"
                     /translation="LNFAIGVATGIVMEFQFGTNWSTYSRFVGDVFGSALAAEGIFAF
                     FLESGFLAILVFGWDRVGPKMHFFATLMVSLGSIFSSIWIVVANSWQQTPAGHHVVPV
                     LRDGVPLLRDGQPVMRAEITDFWAMVFNPSTVHRLVHVWIGAFLVGAFFVMSIAAWYL
                     LKGRHTEFARRSFDGALVLATVSALAMLVSGHGQAGMVYRHQPAKLAAFEGHYVTGPA
                     DLSLFGFPDDATQTVKLRVAVPGGLGLLLHGDPGRPVVGLDRFRPEDRPPVGLSFQSY
                     HLMVALGMAFIGLTLLASFLRWRGTLYETRWLLWLFVPAVVLPIVANEVGWAAAEVGR
                     QPWIVHPPVTWNADGTDVVVGPEGRVVYDERVGLRTLEAASPNVSAGQVLGSLVGFGF
                     IYLALGAVWVFVLHRKIQHGPDEAPPGEGAADDGALAAAGLRAVHEGRLTGGEDRG"
BASE COUNT         1521 a         4237 c         4216 g         1558 t
ORIGIN      
        1 gcagagcttc ccgcgggaga gcgggtgctc ggggttcccg acgagcttcg tgaccttgcc
       61 gtccttcacg tgggcgagga cgccgcactt ccagaagcag agctcgcaga aggtcgggac
      121 aacctggtcg ccgtcggtgc ccgggtcgtg gacgacgtcg gggtcgagcc cccaccactt
      181 cgtcgtcagc cccgacccga gggccgcggc cccgaggccc gcaccgccga tctggatgaa
      241 ctctcgccgg gtcgccgtca tcttcgcctc cggtggtctg gaagatcgcc gccggcccga
      301 tatcaagaaa cgcgccagga ggcgtcaggc ccgagaatgc tgctctgcaa cgctgtcggc
      361 gcgaacctga gagatcgtcg ctgcgacaag ttcattcatg tgcgacagga tgtttcgcgc
      421 tgtggcgccc cgaatgaaac acggtgtttc gcgcggatac cctcttccgc cgggatgcgg
      481 acgggtcgcc gggcggggcc ggctcccgta cgatgaggaa gtgatccgct cgagccgcac
      541 gccggtctgg atcgtcgccc tcgccgtcgt cttcgcctcg gcgcttctcg ggacggccgg
      601 cctggcgtcg cgaacgcttc ggcagcggga cgcggcggtg cgcgacggcg tcctctcgcg
      661 ggcggcgcac gagctcgagg cgagcctgcg gggcgcgacg cccgaggagg cgtcgaccgt
      721 tctccaggag ttcctgtcgc ggtacggggc gtccgtcgac ggggcggcaa tcgtcggacc
      781 ctcaggcgtc ctggccagcg ccggcgtgga ctcgacggac ggctacgagg taacggcggc
      841 gctcggtcgc gagtggaggc cggccctgct cggggcggag ccgggctccg gagccggctt
      901 catgggcgga cggggacccg gtccgggcgc ggggcgggga atgccgccga tccgacttcg
      961 gctgcggcct tcgcccgcgc tgggtcgcgc cccggccctc gccgggctcg tcctcgccgg
     1021 cggcgccgcc gcggcgctgg ccctcgtcgg cctcgcgctc gtcgccgccc ggggcctcgc
     1081 cgagcgagcg aggagggagg cggccgaggc cgaaggtcag cgtctcgcaa ccgtcgcgag
     1141 ggcgggggcc ggcctcgcgc atcgcctccg gaacccgctc gcggtcgtga aggggacggc
     1201 ccagctcctc gacgggcgcg tgccggagag cgaacgggag cgcgtcgtgc ggatcgtgac
     1261 cgccagcgag cggatggaga cgatcctctc ccggctcctc gacttcgcgc gcccgcccga
     1321 cccgcagccc gtctcgttcg acctcgccgc gctcgcccgc gaggtcgcct cgcgcggcgg
     1381 cgaagggacc gcggcagcgg tggccgtccg cgccgaccgg ccggtcccgg tgcgcgccga
     1441 ccgggagcac gtcgagacga tcctggagga gctcctcgcg aacgcccgcg ccttcgaccc
     1501 gaacggaacg ctcgaggtga cggtccgggc cgaagggaac cgggcggtcg tcgaggtcgc
     1561 cgaccggggg ccgggcctcg acctcgatcc ggcgagcgcg ttcgagccgt acgtgacgtc
     1621 gcgtcccgac gggaccggcc tcggtctcgc catcgtccgg tcgctggcga cggcgaacgg
     1681 cggcgccgcg tccctcgcgc cgcgttccgg gggcggctgc gtcgcgacgc tcgcgctgcc
     1741 cggagaggga gcctgacgtg gcgcgccttc tcgtcgtcga cgacgagccc tcgtttcgcg
     1801 cgctcctgcg cgacatcctc gagggagcgg ggcacggcgt caccgaagcg cgggacggag
     1861 cggaggccct ggctttcctc gagaggacga gcttcgacct cgtcctgacc gaccggagga
     1921 tgccggtggt cgacggcctc gagctcctcc ggcgcgtccg cgcacggacc tcgccgccac
     1981 cggtcgtcgt cctgaccgcc cacggctcga tcccggaggc ggtggaggcg gtccggctcg
     2041 gcgccgccga ctacatcacg aagccgctgc cgtcgccgga ggcgctcgtc gacctcgtcg
     2101 cgagcctcca gccgcgcgag gacgacggcg agctcgtgac ggccgacccc gcgatgaagg
     2161 agctgctcga gctcgtcgac cgggtcgcgc cgcgggacgt cgccgtcctc gtgaccggcg
     2221 agtcggggac ggggaaggag ctcgtcgccc gccggctcca cgcccgctcg ccccgcgcga
     2281 aggggccctt cgtcgcggta aacgcggcgg cgctccccga gacgctcgcc gagagcgagc
     2341 tcttcggggc ggagcggggc gcgttcacgg gcgccgacca ggcgcgcgcc gggcgcttcg
     2401 aggaagcctc cggcgggacg ctcttcctcg acgaggtggg ggagctgccg gtcgggctcc
     2461 agtcgaagct cctccgggtc ctcgaggagc gggtcgtgcg gcggctcggg gggagccggg
     2521 acctgccggt cgacgtccgg ctcgtcgccg ccacgaaccg cgacctcacc cgcgagacgg
     2581 aggagggcgg gttccggcag gacctcttct tccgcctcgc cgtcgtcgtc gtgaacgtcc
     2641 cgccgcttcg cgagcggccg gacgacgtgc cgctcctcgc gaggcacttc gcccagcggc
     2701 tcgccgcgcg gcacggggtg cccgtcccct cgctcgcgga cgacgccctc gcggccctct
     2761 cggcgcacgc ctggccgggg aacgtccggg agctgaggaa cgtcctcgag cgggcggtcg
     2821 tcgtccgggg aggagagccg attcgcggcg ccgacctcgg cctcgccccg gcctcgtcgg
     2881 cgttcgcgcc gctcgaccgc gcccaccgcg agcgggaggc gctcctcgcg gcgctccggc
     2941 gaaccggagg gaagcgcgag gaggccgcga agctcctcgg catctcggtg cggacgctct
     3001 actaccgcct caagcagtgg gggatgggct gagcccttct ccgggaggga cgggcgcccc
     3061 tgcagcgccc gtgcagcggg actgcggact ttgcagtccc ggacctctcc gagatggccg
     3121 gcggcggcgg gaaagtcgct ccgcctgaat cacttcgctg cgctccgccc ccgcgtgcga
     3181 cggctggcac cgggcgtgga tgaggggcga cgaggcggcc cgagaccgtg gccgccagca
     3241 ccgatcaagg agccgaacat gaagaagacc gctctcctcg ccgccctcct cctcgcgacg
     3301 ccgctcctcg cccagcccgg tccccgcgga ggccagggga acggcccggg cgccggcgcg
     3361 ggtgcgcccg ctgcgcccgc ggcgagcccc ttcgtgacgt acttcgcctc gcttccgaag
     3421 gagccgctcg gctccgccga ggccgcggag atcgcgttcc tccgcgaaga ggagaagctc
     3481 gcccgcgacg tctaccgcgc gctcttcgcc gcccacggcg accgcgcgtt cgcgaatatc
     3541 gccgccgcgg agcagcggca catggacctc gtgaagctcc tcctcgaccg ctacgagctg
     3601 gccgacccgg ccgccgcgac ggcgcccggc gagttcgagg acgcgcggct cgcggcgctc
     3661 tacaccgacc tcgtggcgaa ggggaaggtc tctctcgtcg aagcgctgaa ggtcggcgcg
     3721 acgatcgagg acctcgacct cgccgatctc gcgaaggcgc tcgccgcctc cgacaaccgg
     3781 gacgtcgacg ccgtgatgca gaacctcgcg aagggttcgc gcaatcacct ccgcgccttc
     3841 gccggtcgcc tccaggcgct cggcgctccc tacgccgccc ggtacctccc ggccgacgac
     3901 gtggcggcga tcctcgccgc ccctcgggag cggggcgcgg tcgacgagat cggcaagctc
     3961 ctgccgggga ccggaccggg tctcgggcgg atgggccgtg gtcgcgggcc gggagcgggc
     4021 gccgggaacg gcaccgggac gtgcgacggg acggggccgg gcgctggcac cggtccggga
     4081 gcgggcttcg gccccggcga cggcacgggc gccggctgcc ccaaccggta gacgcaacgg
     4141 cgcgccgcgc ctttcgcggc ccgaggacgg acggggggag aaggtttcct tctctccccg
     4201 ttccgcgtct gcggcctgcg gcggccgtcc cgccgcgggc agacccgata atcgggaatg
     4261 ccctcggaca aggccctcct cctcacagcg atgggcgcct acctggcgct cacgatcggc
     4321 gtcggcctct ggtactcgag gcgcagcgcc cggagcgccg aggcctactt cctcggggag
     4381 cgcggcctcg ggccgtgggt cgccgcgatg agcgcggagg cctccgacat gagcggctgg
     4441 ctcctcatgg ggcttccggg cgtcgcctac ttcgcggggg cgggcgaggc ggcctggacc
     4501 gcgatcggcc tcgccctcgg cacgttcgcc tgctggagcc tcgtcgcgcg gcgcctcagg
     4561 gcccactcga tcgtcgcggg gaacgcgttc acgctcccgg cgttcttcag cgcccgcttc
     4621 cacgacgacc ggcgcctcct ctcgacgatc gccgcgggaa tgctcctctt cttcttctcg
     4681 atctacgtcg gcgcccagtt cgtcacgttc gggaagctct tcggctacgt cctcggcatg
     4741 gagggcttct acgcgcggat ggtcgtcttc ggcgcgctct tcgtcctcgc ctacacgttc
     4801 atcggcggct tcctcgccga gacgatgtcc gacttcatcc aggggacgct catgttctgc
     4861 gccctcgtcc tcgtcctttc ggtcgggacg gcgaaggcgg gcggcgtcgg agcgctcctc
     4921 gccgagctcg gggcgatccc gcgcttcacc gacctcttcg gcgtcgcgac gccgctcgac
     4981 gccgccggag cggtcgtgac cgccgcagcc gggaacgcgc aggcgctcgt cggcggggtc
     5041 cccgcgtttg gtcccggagt cggctacggc ttcctcgcga tcctctcctg cctcgcctgg
     5101 ggcctcgggt acttcgggat gccgcaggtc cttctccgat tcatggcgat cgagaagccc
     5161 gcgatggtcg cgcgcgcccg cgcgatcgcg gtcacctggt gcgtcgtctc gctcctcgcc
     5221 gcggtcggca tcggcctgat cggccgcgcc ctgatgcccg cccggctcct gacggcgagc
     5281 gcggcggaga cgatcttcat ccacctcgcg gtcgacttct tcccgccgct cctcgccggg
     5341 ctggtcctct ccggcatcgt cgccgcctcg atcagctcgg ccgattccta catgctcatc
     5401 gcctcctcct cgctcgcgaa cgacctcgtt ccgaacttcc gggccgagcc gctttcggac
     5461 cggtcgatcc tcggactcgc gcgcggcacg caggtcgcgg tgaccgcctt cgggatcctc
     5521 gtcgccctct cggggaacga ctcgatcttc cgcgtcgtct cctacgcctg ggccgggctc
     5581 ggcgccgcgt tcgggcccct cgtcctcttc tcgctcttct ggaggcggac gacgctcgcg
     5641 ggggccgtcg cggggatgct gaccggaggg atcgccgtcg tcgcctggaa gaacctcttg
     5701 gcgccgctcg gcggcgcgtt cgccgtctac gagctcctcc cggcgtttct cctctcctcg
     5761 ctcgcgatcg ccgtcgtcag ccgcctgacg cccgcgccgc cggcggaggt cacagacgcc
     5821 ttcgacgagg cgcggacctt cccgggctga gcgccgggac tacttcggag ccggcgggag
     5881 gacctcgagc tcgatcgcga cggggaaggg gagccccgcg tcgggcgtcg gcctcacgtt
     5941 cgagacggcg tagacgagct cgaggcgccg cgggcccggg gccgtcctct tcgggaccct
     6001 cacctcgagg aggctcgtcc cgttcgcggc gacgacgagc ttccccggga cgacgacctc
     6061 gcccgccgct ccctccccct cgaggacgag gcggaggtcg gagtcgttcc ggagccgcac
     6121 gtatccctcg tcgcccgcgc ggacggcgag cttcgggttc tcgatcgtca cggcggcggc
     6181 gacgagcggc cggagctcct tctcgcgccc ggcgagcttc ccgtcggccc aggcggccgt
     6241 cctccgcgcg aagagcgcct cgcggatccc ctcctcgctc cgctccttcg cgaagacgag
     6301 cgtgacgggg cgacggtccc cctcgtggac gtgccaggcg aggttcgagg ggtcgtggat
     6361 gtcggaattc gcgaggatcg cgaggtcctt ctcgagcgcc caaccgtgcg cttccgggta
     6421 gtactcgcgg tcgttcgcca cctcgatgcc gtccatcagg ccggccgcga ggatctcgtc
     6481 gtgctcgggg taccagcgcg cgacgccgtc cttctgctgc ccgcgccagc cggggtggtt
     6541 ccagaagaga aaggccccct gcgcccgcgc cgctctcagc gcgtccctcc actcgacggt
     6601 ttcgagcggc ttcgacgtct tcaggaagat cgcgttgaag tggccgggcg gcatcttccg
     6661 cgtcacctcg gagccccgga cgacgaggac cccgagcttc tccccgagcg gcgcggcgat
     6721 ctcgaagggg cggtcgtggc tcgtcggtag gtccgcctcg tgcggctggt actcgatgtg
     6781 gtcggtgatg gcgatcgcgt cgtacccctc gcgccacgcc tcctcggagc ggatgtcggg
     6841 ccagacggag ccgtcggaga agaccgtgtg cgtgtggaag tcgcagcgga gcgtgacgaa
     6901 gcccgggatg tcggggaccc ggagcggcct gcgaacgcgg acctcgtgcg ccggagcggt
     6961 ccgcgcgagc gcagcgacgg tggcgagaac ggcgagaccg gcgagccgga gaggggcgga
     7021 gcgacgcggc atgcggacct cctcgcggga gcgacggtcg attctccggc cgcgtccggg
     7081 tcgtggcaac ggaccgccgc ggggccgcgt gctaccttcg atcgatgccc gacgcgctcc
     7141 ccgacgtctt tcacgagctc gaggccgacg ccctccctgc cgccgcggag acgctcgtct
     7201 cgatcgcggc cgactacctg gccgagacgc gctccggcga cggccccgtc tcgactccgc
     7261 tcaccccgcg ggagctcgcg cgcctcttcg acgagcccct gccccggcgg ggaaggcccc
     7321 tcgccgaggt cgccgagcgc ctccgacgcg aggtcgtccc cgcctcgaac cgcctctgcc
     7381 acccgcgctc gatggggcac caggtctcgg cgcccctccc cgctgcgatc tggaccgagc
     7441 cgctggccgc ggcgctcaac cagtccctcg ccgtcttcga gatgtcgccc gccgggacgg
     7501 cgatcgaggg ccgggtcgtc cgctggatgt gcgacctcgc gggattcggg cccgcagcgg
     7561 gagggacgtt cacctcgggc gggaccgagg cgacgttcac ggcgctcctc gccgcccgcg
     7621 cggcggcgct ccccgacgcc tggacgaggg gagtcggggc ggagccgccg gtcgtcctgc
     7681 acggggagca cgcgcactac gccgtctccc gcgccgttgg cgcgctcggg ctcgggaccg
     7741 acgccgcggt ggcggtcccc tcgcgcgcgt tccgcatgga cgtcgccgcg ctcgaggccg
     7801 agctcgatcg gcagacggcc cgcggacagc gggtgatggc cgtcgtcgcg acggccggct
     7861 ccacggcgac cggctcgttc gacgacctgc cggcgatcgg ccgcctctgc gaggcgcgcg
     7921 gcgtctggct ccacgtcgac ggcgctcacg gcgcctccgc cctcctctcc tcggctcacc
     7981 gcgggcggct cgacgggatc gcgggggccc gctcgctcgc ctgggacccg cacaagatga
     8041 tgctgatgcc ctctcggcgg gcgtcctcct cgtgcgcgac gagcgcgatc tcgacgccgc
     8101 gttcgcccag cgggccccgt acctcttcca cggcgcggag ggggagcgga gccccgacca
     8161 ggggaagcgc agcttccagt gctcgcggcg gctcgacgcg ctgaaggtct gggtcgccct
     8221 ccaacgctac ggcgcggacg gcctcggcgc tctctacgac ggcctttgct ccggggcccg
     8281 gacgctccac gcggcgctcg cggcccggcg cgacttcgag acgctccacg agccggagtc
     8341 gaacatcctc tgcttccgct gggtcggcgg cggggagctc gacgccgaac gcctcgacgg
     8401 gatcaacctg cgcctccgcg aggcgttcaa ccgctccggg gagggctgga tcacgacgac
     8461 gctcctgggc ggacggcggg tccttcgcgc gacgcagatg aacccgcgga cgacggcggc
     8521 cgacctcgag cgggtcgtcg aggggctcgc gcgaacgggg cgggagatcg ccgcccggag
     8581 cgcggatcgt ccttgaccaa tacgggcata tgcccgaaac tgccgggtgg cccgcggtgg
     8641 agatgcgggg gcccgaacgg gagccccgcg gatcgcggcg gccggaagga gccccgatga
     8701 acctctgcat cccggtcagc gccgaccgcg gcctcgagag cccggtctcc gggcacttcg
     8761 gttccgcgcc gctctacctc ctcgtcgact cggagactcg cgcgacgcgc accctcacga
     8821 acgcccgggc cgtccacgag cacggcgcct gccgtccgct cgacgcgctc gcaggggagc
     8881 gggtcgacgc cctcgtcgtc ggcggcatcg gcgcgggagc cctggcaaag ctccgcggcg
     8941 cggggatccg cgtctttcgc gcgacggcgc cgacggcctc cgcctgcctc gacgccttcc
     9001 tccggaacga ggtcgaggag atcgaccccg ccggagcgtg cgccggccac gagcacgacc
     9061 acggcgcggg gcacgcagcc ggactcccga cgcggacctg agctggacgg ggacacgggc
     9121 cggcgacggt ggggcgccgg ctccgctcag tagctgtgct cgctcagctt caccttcccg
     9181 cggaacgtcc agtagatgac gccggaatac gtcaggacga acggcatccc gacggccgcg
     9241 atgaccagca tgatcccgag cgtcttcggg ctggaggccg cgttgaagac cgtgacggag
     9301 cgcgacgggt cgccggcggc cgtcacgagg ttcgggtaga gcgcgaggcc gaagagcgcc
     9361 acgagccccg cgatcacgcc cgccgaggag aggaaggcga ggccgggccg gtcgcggtag
     9421 aggcagcgcg ggatgttcgc gacggcgagg atcgtcccga cgacgacgac gatcgcccac
     9481 gggaagcttc ggaagttctc cgtagcccgc gggatgaagg cgaccgtcgc caccgtcgtc
     9541 agcatgaaga agaccatgaa gaggccccac gagcgccaca tccactcgcg gaggcgctgc
     9601 tggaactccc cttcggtctt caggtagagg aagagcccgc cgtgcatcgc gaagagggcg
     9661 acggtcatcg ccccgacgag gagcgggtag gggccgagct ggtcgacgac cgtcccggtg
     9721 acgttgccgt tcgcgtcgat cggaacgccc agcatcgcgt tcccgacggc gactccgaag
     9781 aggagcgtcg ccgtgaacga gccgccgaag aaggcccagt cccagaagcg gcgccacgcg
     9841 ggcgaggcca tcttcgagcg gaactccatc gagacggccc gcgagatgag cgcgacgagg
     9901 aggagcatga aggccgtgta gaagcccgag aagaccgtcg cgtagccctc ggggaaggcg
     9961 gcgaagaggg cgccgccgaa cgtgacgagc cagacctcgt tgccgtccca gagcgggccg
    10021 atggagttca tcgagatccg gcgctcgagg tcggtccgcg ggacgaacgg atggaggatg
    10081 ccgacgccga ggtcgaagcc gtcgaggatc gcgtagccgc agaggaggac cccctcgagg
    10141 acgaaccagg tgatcgcgag cgcttcgaaa gacatcgccg cctcctcagc cccggtcctc
    10201 gccgcccgtg agccgcccct cgtggacggc gcggaggccc gccgcggcga gggctccgtc
    10261 gtcggccgcg ccctccccgg gtggcgcctc gtccggtccg tgctggatct tccggtggag
    10321 gacgaagacc cagacggcgc cgagcgcgag gtagatgaac ccgaagccga cgagggagcc
    10381 gaggacctgc ccggcggaga cgttcgggct cgcggcctcg agcgtccgga gccccacgcg
    10441 ctcgtcgtag acgacgcgcc cctcggggcc gacgacgacg tccgtcccgt cggcgttcca
    10501 ggtcaccggc gggtggacga tccagggctg gcggcccacc tcggcggccg cccagccgac
    10561 ctcgttcgcg acgatcggca ggacgacggc ggggacgaag agccagagga gccaacgcgt
    10621 ctcgtacagc gtgccgcgcc acctcaggaa cgaggcgagg agcgtcaggc cgatgaaggc
    10681 catcccgagc gcgaccatca ggtggtagct ctggaagctg aggccgacgg gcgggcggtc
    10741 ctccgggcgg aagcgatcga ggccgacgac gggcctgccc gggtcgccgt gcaggaggag
    10801 gccgaggccg ccgggaacgg cgacgcggag cttcacggtc tgcgtcgcgt cgtccgggaa
    10861 cccgaagagg ctcaggtcgg cgggcccggt gacgtagtgc ccctcgaagg cggcgagctt
    10921 cgccggctgg tggcgataga ccatccccgc ctgcccgtgc ccggagacga gcatcgcgag
    10981 cgccgagacc gtggcgagga cgagcgcgcc gtcgaacgag cggcgggcga actcggtgtg
    11041 gcgccccttc aggaggtacc aggccgcgat cgacatgacg aagaacgccc cgacgaggaa
    11101 cgccccgatc cagacgtgga cgagccggtg gaccgtcgac gggttaaaga ccatcgccca
    11161 gaaatcggtg atctccgcgc gcatcaccgg ctggccgtcc cgcagcagcg gcacgccgtc
    11221 gcggaggacc gggacgacgt ggtgcccggc cggcgtctgc tgccagctgt tcgcgacgac
    11281 gatccagatc gacgagaaga tcgatccgag cgagaccatc agggtcgcga agaagtgcat
    11341 cttcggcccg acgcggtccc acccgaagac gaggatggcg aggaagcccg actcgaggaa
    11401 gaacgcgaag atcccctcgg ccgcgagcgc cgagccgaag acgtcgccga cgaaccgcga
    11461 gtaggtcgac cagttcgtcc cgaactggaa ctccatgacg atcccggtgg cgacgccgat
    11521 cgcgaagttg ag
//
LOCUS       DAOTEZ010000088        11529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3936_length_11529_cov_4.976381, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000088 DAOTEZ010000000
VERSION     DAOTEZ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11529
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3936_length_11529_cov_4.976381"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="P5164_06525"
     CDS             complement(<1..469)
                     /locus_tag="P5164_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43586.1"
                     /translation="MSNVLTFQRSAAAVRLMVLVAGAGYGLTKSLSALASGLDATRQR
                     PVAIAVAEPVPRKSDQAIQLCASIGLAVTPYGGRAEDAVCDRIGGDAPIVAFLDSAVS
                     TRRILTAPAAQHRPVLVYFVLLVNQTVLAFRMVFIPGQDAIAQRDEQIVAVGHA"
     gene            complement(532..957)
                     /locus_tag="P5164_06530"
     CDS             complement(532..957)
                     /locus_tag="P5164_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43587.1"
                     /translation="MKRSPYFAPLLIALVLLVIALVVIRTDGADTEDESLPHRLAQGA
                     IYAVLPVGTAFIAEFLLRKFNPASRAAREHKRSKKALDGVTSKKRKAEKRIKEIHDDY
                     ELYLYWVARIRGRFYRFFEPARARAGHTGPAIPSVNPNP"
     gene            complement(954..1424)
                     /locus_tag="P5164_06535"
     CDS             complement(954..1424)
                     /locus_tag="P5164_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43588.1"
                     /translation="MFRKGMSFKSFLSFYVRYRRNLIPADIEKDAEVNIDSCVARTLS
                     ILGKPRPFDPVNDPEHAKLAEDANTDSVVEERAADLELQERERAKHTRRWVLVPGLTL
                     CLLVEAVSCVQVFAALGIEMPERAFFGLGLAAAIFALIYFTQQQATPKEAEVSE"
     gene            complement(1435..2277)
                     /locus_tag="P5164_06540"
     CDS             complement(1435..2277)
                     /locus_tag="P5164_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43589.1"
                     /translation="MTLTFPDTTRIPRFVLSVLLLSLVAALGSGCGGDGGDGLSSAAT
                     KLGLKDVPTLPPEVIDVLCDASIDTPCTEATLAQTLESVLTYAASRPNSHVRLWRVGR
                     DVSDTFLLAEQVALPPPKQKKAMREARRKWVDSSLRFFLKAAEPMLQGKRATRSALFE
                     SLTKVALAEVPGNTTRVVVFLTDGLEVSGYADFEASELAPVNDLSARLHADRVLAPGI
                     LKDTKVYLAFADMSRAVRNRGRSSIPRLTAITENWKQLLREAGASEVVITTGVAPIGA
                     RMGE"
     gene            complement(2494..3108)
                     /locus_tag="P5164_06545"
     CDS             complement(2494..3108)
                     /locus_tag="P5164_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43590.1"
                     /translation="MMRFLGMGCNLVWLTQRGADALVDGGFATADQLFPRTRVVAAKD
                     LAHHLYIVDLCLLALRGIPRAYQSVEPAIAIQRRLQPQPEAIPDVLLSSPSKVTGRRN
                     LLAFEVDLGTEAVQVFGKKLEAASRVLTEWANGGAANVVILTRGSGRASSIERELAAR
                     AVAIRVPMVVRQLPRGGSLNREALAAVISRRGTSSDGTPSGERL"
     gene            complement(3441..4037)
                     /locus_tag="P5164_06550"
     CDS             complement(3441..4037)
                     /locus_tag="P5164_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43591.1"
                     /translation="MDARRIPLLRLLPAPPVVFSEGTSPVHERGVDDIDPLALDRMTR
                     SSVSDGWGTGQGSYDSEFTLPEREVPRLVPAVTSPAAKSLGHSLPRIPMRSTRRLDLL
                     VPIADNDGHPFNPEDLSSFEGHLIALAGGFTRRGRISGVWQSPSGQVFTDESLAYSVT
                     VPSDVAESVSADISRFIQTRFHQEAAWVEATPTFVAAL"
     gene            complement(4040..4435)
                     /locus_tag="P5164_06555"
     CDS             complement(4040..4435)
                     /locus_tag="P5164_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43592.1"
                     /translation="MTSAFTLEQRRVERLSVVTLISFLVIFLGPFPLTTMPYSIRFRV
                     PLPPPEIGTGGLVRFLLAIRELAVDRFSGGMCSWRDGHPADGSPPDPCVTVLLTFDDT
                     VNPLEVTRALDRLYDHAEAIVLEQQETEE"
     gene            complement(4432..4740)
                     /locus_tag="P5164_06560"
     CDS             complement(4432..4740)
                     /locus_tag="P5164_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43593.1"
                     /translation="MSDPTKKVSSIFSTLVLYVLASPIFALKAIGSFVRSYRTATLVR
                     AGFLRCSACGRGNRLDVLVTCPRCSFAEFRSLALPCSECGYVPGFLSCTGCGASVRLP
                     "
     gene            complement(4733..6394)
                     /locus_tag="P5164_06565"
     CDS             complement(4733..6394)
                     /locus_tag="P5164_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43594.1"
                     /translation="MSAVNFLSFETAQSILPLLTAAVLLPWEARLRRLTGERALAYEK
                     RKRQPAELPPPMDNSAATLSRLDGSDAVVVGTSAGRPVRIKEEIAWRSGLFVGCSGAG
                     KTRGVVGILKQLMHAMTTGKPCEIAVIDAKNETVDLIRQVVGGWLLTLPDAQKEWLRR
                     HVRVFMFGRDCVTPLRPYDNLGSIFTDAYSAGFRTTVTVDAGTADYTDGTRHARAMFD
                     RVMTDLRWPLSSPVAVRFFTDTAFQIFVASRVRDGHLRSFIQTMPITVPKGTAAAVCR
                     RVDREMSDPHIRAAKGVPPAALDELLPAVEPGLTLARVGPDSVLTPTVAQELCLLRYF
                     ELLASVPTRSPKRPLLIVAEELAATVAGSPRLVLPITQLLRMSRSFGVALWALAQDFE
                     NAATVEMANAFALNARFVVAFQSREEAAWLAPHIPPDFAPGVSDAERKRAFLREMESL
                     PPRSFFLWVKGMDLLRCQALEVPDPAVEFGRSAEELTEVFDREIGSRSTITLEKAEEL
                     IAAFEADVLGKRDVAPAPEPKARPTFSSMEDFLRHVEEEEEDGDV"
     gene            complement(6396..6761)
                     /locus_tag="P5164_06570"
     CDS             complement(6396..6761)
                     /locus_tag="P5164_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43595.1"
                     /translation="MASAIAALIASGCVLVVLGGAIKVAFGNATILKAGGILLLIALL
                     PTLAVGLFTGMSASTPNGGGSSSLGSFLAVIGVLAIVSVIAYLYLMAKGVLGSKRNGD
                     SDRGGRRGGYRLDDSESED"
     gene            complement(6763..6957)
                     /locus_tag="P5164_06575"
     CDS             complement(6763..6957)
                     /locus_tag="P5164_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43596.1"
                     /translation="MSLDGVATDSASVELDDAAAFLMFIALKRTLTDGAPAKRKGRRG
                     ASVTNDETGGTDAASETEDV"
     gene            complement(6987..7334)
                     /locus_tag="P5164_06580"
     CDS             complement(6987..7334)
                     /locus_tag="P5164_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43597.1"
                     /translation="MKISLSDRLAICFVCFIEWLVLEFRFTPLSAATVERDESPSNER
                     STTMKSITISIKCGGMLRTETRRFNERDYSEAEVYADVCRNATHLLEDCIEFNPNLNV
                     HVQGLNTRSQKPA"
     gene            complement(7797..8990)
                     /locus_tag="P5164_06585"
     CDS             complement(7797..8990)
                     /locus_tag="P5164_06585"
                     /inference="COORDINATES: protein motif:HMM:NF016643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43598.1"
                     /translation="MSTETPEASSRAELAPQEAVLRFLESTGRPSEARLYVDLFRARP
                     REQFAAIAIDAHVVADAASGVVQDLRFLAALELFPTVVLGLFQPADAARHAASLCRRL
                     EAEGVRHEVVPAGSERVVERLAACARGAGVPVLVFPPVEGEDADGRLDRLGVLLRELG
                     ARKLLFLHRPGGLRQGGLLVPIVNLTTEAAGLLASGELSRKESLILAHARRLCEESAP
                     PSFSVAVTSPLNLLRELFTVKGAGTLLRRGARIERHDGWAGVDLSRLRALLASSFRRP
                     PSDAFFGRTPGRVYVEESYRGCAVLVATPLGAYLTKFAVSAEAQGEGIARDLWDALAA
                     ESPAVFWRARPSNPIGDWYAKLADGLVRLSDWTIYWKGVPPAEIPAVVEWTLAQPVDI
                     PRDGE"
     gene            complement(9067..9948)
                     /locus_tag="P5164_06590"
     CDS             complement(9067..9948)
                     /locus_tag="P5164_06590"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HRY43599.1"
                     /translation="MRRARIESLGVSPPRRGLLRWGALKHTVEAGKRCLAASKYRPAD
                     VRVLVNAGVHRDGHVCEPAIACYVQHALDINVDFHGRRTLSFDLLNGNVGMLSAAHVV
                     SAQMLAGEVAVGMVVAGEANSDRRPDAESTIPASGAAMLLDLSPTSGTGFGAFAFDTR
                     EERADLYTSVVSLKEPHGKLLIRRRAELEEAWLEGAAAAATEAFERDGLTPADVDLVV
                     PAQVSPAFLSRLPAVLGVPREKVADFTATLPDTLTTSFVLALDRARSEGALAPGRRAL
                     LLAFGSGVTVGAATYRF"
     gene            complement(9945..11009)
                     /locus_tag="P5164_06595"
     CDS             complement(9945..11009)
                     /locus_tag="P5164_06595"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HRY43600.1"
                     /translation="MTRKIRIESLGVRLPEKVVTTEELMASCRRRPRLDLERITGIVT
                     RRVAVDEYAGDLAVGAARRALAMSRYAPEELDAIVCTSISKHNRPDEFTFEPATAVLV
                     RRAVGAKKALVFDVVNACAGLLNGIWVLQGLIRSGAIRCGMVVSGEQNMPLARTAARE
                     VRHSLDGQLAALTLGDCGAAVIVDASPDEAVGFHWLDLVSGARHDHYCWSKPSSRGSG
                     GILVTKARGLQIEGNRNFPAYLKQALDATGWDWSDVHHVIPHQVSTRAIRQGIRAISR
                     YMGCDLPDVFLSHAETFGNTTTASHALALHDFILRGTIRSGQNVLLVSGASGIVISHA
                     TFTFDDLPDRYRAHVNGAPA"
     gene            complement(11050..>11529)
                     /locus_tag="P5164_06600"
     CDS             complement(11050..>11529)
                     /locus_tag="P5164_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43601.1"
                     /translation="RPGAIWGPGDPNVVPRIVRLLRRGAMPYVGGGRNLVGLVHVENL
                     VRGLLLAGEAEAAAGEIFHLTDDEEITAREAVDGIADALGVPRPRLSIPFPVVLAVAA
                     LVEGTARAARLRRPPPLTRYGARFVACHARYDLAKARRELGYAPAVSFREGAAELFR"
BASE COUNT         1922 a         3681 c         4229 g         1697 t
ORIGIN      
        1 ctgcgtgacc gacggcaact atctgctcat cgcgttgcgc gatggcatcc tggcccggga
       61 tgaagaccat cctgaaggcc aggaccgtct ggttgacgag gagtacgaag tagaccaaga
      121 caggccgatg ttgcgccgcg ggagccgtca aaatgcgccg ggtggacacg gcggagtcga
      181 ggaaagcgac gatcggggca tcgccaccga tccgatcgca cacggcgtcc tcggcccggc
      241 caccgtaggg cgtcaccgcg aggccgatgc tggcacaaag ctggatcgcc tggtcggact
      301 tccgggggac gggttcggcg acggcaatcg cgacggggcg ctggcgtgtg gcgtcgagtc
      361 ccgaggcgag cgcgctgagg ctcttcgtga ggccataccc cgcgcccgcc acgaggacca
      421 tcagcctgac ggcggctgcg gagcgctgga aggtgagaac gttcgacatg ggagcctcct
      481 tggagaaaga aggggggtgg aaggacccca cccccctcag tacctgacgc gttacgggtt
      541 cgggttgacg gaggggatgg cgggtccggt gtgtccggcg cgtgcgcgcg cgggttcgaa
      601 gaagcggtag aagcgccctc tgatcctcgc gacccaatac aaatacaact catagtcgtc
      661 gtggatttcc ttgatgcgct tctcggcctt gcgcttcttc gacgtgaccc cgtcgagggc
      721 cttcttgctc ctcttgtgtt ccctggcggc gcgcgaggcg gggttgaact tccgtagcag
      781 gaactctgcg atgaacgccg tccccaccgg gaggacggcg tagatggccc cctgtgcgag
      841 ccggtggggg agggactcat cctcggtgtc cgctccgtcg gtccggatga cgacgagcgc
      901 gatgacgagc aggacgaggg cgatcaggag gggcgcaaaa tagggtgacc gtttcactcg
      961 gagacctccg cttctttagg ggtcgcttgc tgttgggtga agtagatgag ggcgaaaatg
     1021 gccgccgcga gcccgaggcc gaagaacgcc cgctcgggca tctcgatgcc cagcgcggcg
     1081 aagacctgga cacagctcac agcttcgacg agaaggcaca aggtgaggcc gggcacgagc
     1141 acccaccgtc tcgtgtgctt cgcccgctcc cgttcctgta gctcgagatc agccgcgcgt
     1201 tcctcgacga cgctgtccgt gttcgcgtcc tcggcgagct tcgcgtgctc ggggtcgttg
     1261 acggggtcga acggacgcgg cttgccgagg atggaaaggg tccgcgcaac gcagctgtcg
     1321 atgttgacct cggcatcctt ttcgatgtcg gcggggatga ggttgcggcg gtagcgtacg
     1381 tagaacgaca agaacgattt gaacgacatg ccctttctga acacggcggc ctcctcactc
     1441 gcccatgcgc gcgccgatgg gggcgacgcc agtggtgatg acgacctcgg aggcgccggc
     1501 ttcgcgcagg agctgtttcc agttctcggt gatggcggtc agccggggga tcgaggagcg
     1561 cccccgattt ctcacggcgc gagacatgtc ggcgaacgcg aggtagacct tcgtgtcctt
     1621 gaggatgccc ggagccagga cgcggtcggc gtggaggcgg gcgctgaggt cgttcacggg
     1681 agcgagttcc gacgcctcga agtcagcgta gcccgagact tccaggccat cggtgaggaa
     1741 gacaaccacc cgcgtcgtgt tgccggggac ctcggccagg gcgactttcg tcagcgactc
     1801 gaacagggcg ctccgtgtgg cgcgctttcc ctggagcatc ggctcggccg ccttcaggaa
     1861 gaagcgcagg cttgagtcga cccacttacg ccgggcctca cgcatcgcct tcttctgctt
     1921 cggtgggggg agcgcgacct gctcggccag gaggaacgtg tcggacacat cgcgcccgac
     1981 cctccaaagc cgcacgtggg agttggggcg ggacgccgcg taggtgagga cgctctcgag
     2041 cgtctgggcc aacgtcgcct ccgtgcacgg cgtgtcgatg gatgcgtcgc acaggacgtc
     2101 aatgacctcg ggcgggagcg tcgggacgtc cttgagcccg agtttcgtgg cggcgctcga
     2161 gagtccatcg cccccgtctc cgccgcagcc gctcccaagc gcggcgacga gcgaaaggag
     2221 caggaccgag aggacgaacc ggggaatccg ggtcgtatcc gggaacgtga gggtcatggg
     2281 cgggtcctcc tttgggcttt cgggtggatg gccggggctc ccagggcgag gcgcgacggc
     2341 ggagccctgg cgcgggcgag tccctgcccg tgtccgcgaa ggtcgagcgg cgatggcgac
     2401 gaccccgaga gaagcgcgat ccgatcgtga ggctcgatcc tccgcaggac accggggggt
     2461 ccaaccccca acaagccgtg gccgtgcatc gacttacaac ctctcccctg atggggtgcc
     2521 atcgctcgac gtaccccgac gacttatcac cgcagcgagc gcctctcggt tcagcgagcc
     2581 acctctcggg agctgccgga cgaccatcgg aacccggatg gccactgcgc gggccgcgag
     2641 ctcccgctcg atggacgagg cccgacccga gccccgtgtc aggatcacga cgttcgccgc
     2701 gccaccgttc gcccactccg tgagcacgcg cgacgcagcc tccaatttct tgccgaagac
     2761 ctgcaccgcc tccgtcccga ggtcgacctc gaaggcgaga aggttcctcc gtcccgtcac
     2821 cttcgaaggc gacgacagga gtacatcggg aatcgcctca ggctgaggct ggagccgccg
     2881 ttgaatcgca atggcgggct cgacgctctg gtaggcccgc ggaatgccgc gcagggccaa
     2941 gaggcagagg tcgacgatgt agaggtggtg tgccaggtcc ttggccgcaa cgaccctcgt
     3001 gcgggggaag agttggtcgg cggtcgcgaa gcccccgtcg acgagtgcat cggcacccct
     3061 ctgcgtcagc cagacgaggt tgcagcccat gccgaggaag cgcatcaagc agacccatcc
     3121 acgggccacc caacgcgcca aacggcgcgc gaagttcgac tccttgcagc ccccgtagcc
     3181 ctcgaagcgc agctgcggac cggtcatgca gccgaagcgc ccgagcagca cgaggcccag
     3241 gacgtcgagc gggacgagta cctcgtcgcg tggatcgcga cggggcgtgc ccatccccgg
     3301 aggccgaccc tgcgccggaa tccgctgcgg gagcttcacc tgcgtcggct cgttcgatgc
     3361 cgtcgaaagt gtctcccgca tttcatccag ggcaggggtc gggtcggaaa ctcccgtgag
     3421 gagagcgtta ctcgccgacg tcacaaagcc gccacgaagg tcggtgttgc ttccacccag
     3481 gctgcctctt ggtgaaagcg ggtttggatg aaccgggaga tgtcagccga cacgctctct
     3541 gcgacgtccg aggggacggt cacggagtag gcgagcgact cgtcagtgaa gacctggccg
     3601 cttggggact gccagacacc ggagatcctg ccgcgacggg tgaaaccgcc ggcaagcgcg
     3661 atgaggtggc cctcgaatga ggagaggtct tcgggattga aggggtgacc atcgttgtca
     3721 gcgataggca cgaggaggtc gagcctgcgt gttgaacgca tggggattcg ggggagggaa
     3781 tggccgagag acttggcagc aggagacgtg acggcgggga cgaggcgagg aacctcccgt
     3841 tcggggagag tgaattcgga gtcgtaggag ccttggcccg tgccccaccc gtcgctcacc
     3901 gaggaccgtg tcatccggtc gagcgcgagg ggatcgatgt catcgacgcc tcgctcgtgc
     3961 accggagacg ttccctcgga gaagacgacg ggtggggccg gaagcaacct gagcagcggg
     4021 atgcgtcgcg cgtccacggt cattcctccg tctcttgctg ttcgaggacg atggcttcgg
     4081 cgtggtcgta gagccgatcc aatgcccgcg tgacctcgag cggattgacg gtgtcatcga
     4141 acgtgagaag gaccgtgacg cagggatctg gaggggaacc gtcggccgga tgcccgtcgc
     4201 gccacgagca catgccgccg ctgaagcggt cgacagcgag ctcgcggatc gcgaggagga
     4261 agcggacgag gcccccagta ccgatctctg gaggcggtag tgggacgcgg aagcgaatcg
     4321 agtagggcat ggtcgtgaga gggaaaggac cgaggaagat gacgaggaac gagatcagag
     4381 tcacgaccga cagacgctcg acgcgtcgct gctcaagggt gaaggcgcta gtcatggcaa
     4441 acggacgctt gccccgcagc ccgtgcagga gaggaagccc gggacgtacc cacactcgga
     4501 gcacggcagg gcgagcgagc ggaattcggc gaagctgcag cggggacagg tgacgaggac
     4561 gtcgaggcga ttccctcggc cgcaggccga acaccggagg aatccggcac gaacgagcgt
     4621 cgccgtgcgg tacgagcgga cgaaggagcc gatcgccttc agggcgaaga tcgggctcgc
     4681 cagcacgtag agaacgagcg tgctgaagat ggatgagacc ttcttcgtcg ggtcagacat
     4741 cgccgtcctc ctcctcctct tcgacgtggc gcaggaagtc ctccatcgag gagaacgtcg
     4801 ggcgggcctt cggctccggt gcgggggcga cgtcgcgctt accgaggacg tcggcctcga
     4861 acgcggcgat cagctcttcg gccttctcga gggtgatggt cgagcgcgag ccgatctcgc
     4921 ggtcgaagac ctccgtcagc tcctcggcgc tccggccgaa ctcgacggcg ggatcgggaa
     4981 cctcgagtgc ctggcaacgc aggaggtcca tccccttgac ccaaaggaag aagctcctcg
     5041 gcgggaggct ctccatttcc cggagaaacg cgcgcttccg ctcggcgtcc gagacgccgg
     5101 gagcgaagtc cggcgggatg tgcggcgcga gccacgccgc ctcttcgcga gattggaacg
     5161 cgacgacgaa ccttgcgttg agagcgaacg cgttcgccat ctcaaccgtg gccgcgttct
     5221 cgaagtcctg ggccagcgcc cagagtgcga ccccaaagga gcgggacatg cggaggagct
     5281 gcgtgatcgg caggacgagt cgaggagagc ccgcgaccgt cgccgccagc tcctcagcga
     5341 cgatcaggag cgggcgcttc gggctccgcg tcgggacgga cgcgagcagc tcgaagtacc
     5401 gtaggaggca gagttcttga gcgaccgtcg gggtgaggac gctgtcgggt ccgacgcgtg
     5461 cgagcgtcaa cccaggttcg acggccggga ggagctcgtc gagggcagct ggtgggacgc
     5521 ccttcgccgc gcgaatgtgc gggtcggaca tctcgcggtc cacccggcgg cagactgccg
     5581 ccgccgtccc cttcggcacc gtaatcggca tcgtctggat gaaggagcgg aggtgcccgt
     5641 cacgaacgcg cgaggcgaca aagatttgga acgccgtgtc cgtgaagaac cggacagcca
     5701 cgggggaact cagcggccac cggaggtccg tcatcacgcg gtcgaacatg gcgcgcgcgt
     5761 gcctcgtccc gtcggtgtag tcggccgtgc ccgcgtcgac cgtcaccgtc gtgcggaagc
     5821 ccgccgagta ggcgtccgtg aagatgctgc cgaggttgtc gtacggacgg agcggggtca
     5881 cgcagtcgcg cccgaacatg aagacgcgga cgtggcgcct gagccactcc ttctgcgcgt
     5941 cgggaagtgt gagtagccag ccccctacca cttgtcgaat caaatcgacc gtttcatttt
     6001 tcgcgtcgat gacggcgatc tcgcacggct ttcccgtcgt catcgcgtgc atcagctgtt
     6061 tcaaaatccc aactaccccc cgcgtcttcc ccgctccgga gcagcccacg aaaagtccac
     6121 ttcgccaggc gatctcttct ttgatccgca cggggcgacc ggcgctcgtc ccgacgacga
     6181 cggcgtcgga accgtcgagt cgggagagcg tcgccgccga gttgtccatg gggggaggga
     6241 gttcagcggg ctgccgcttc ctcttctcgt acgcgagcgc ccgttccccc gtgagccgcc
     6301 gcagccgggc ctcccacggg agcaggaccg cagcggtcaa gagggggagg atgctctggg
     6361 cggtctcgaa cgagaggaag ttgacggccg acatttcagt cctcggactc cgagtcgtcg
     6421 aggcggtagc ccccgcgccg accgccccgg tcgctgtcgc cgttgcgctt gctgcccagg
     6481 acacccttcg ccatcaggta gaggtaggcg atgaccgaga cgatcgcgag gacgccgatg
     6541 acggcgagga acgagccgag cgaactcgag cccccgccgt tcggcgtcga agcagacatt
     6601 cccgtgaaga ggccgaccgc caaggtgggc agcagcgcga tcaggaggag gatgccgccg
     6661 gctttgagga tggtggcgtt tccaaaggcg accttgatcg ccccgccgag cactacaagg
     6721 acacacccgg aggcgatgag ggcggcgatg gcggaggcca cgtcagacgt cctcagtctc
     6781 ggaggcggcg tccgtcccgc ccgtctcgtc gttcgtgacg ctggcaccgc ggcgaccttt
     6841 cctcttcgcg ggcgcgccat cggtgagggt ccgcttcagg gcgatgaaca tcaggaaggc
     6901 ggcggcgtcg tcgagctcga ccgaggccga gtcagtggcg acgccgtcga gggacacggt
     6961 gacgcgatgt ccgctcatct cggtcctcag gcgggcttct ggctgcgggt gttcagaccc
     7021 tgcacgtgga cgttgaggtt ggggttgaat tcgatgcagt cctcgagcaa gtgggtggca
     7081 ttccggcaga catcggcgta gacctcagct tcggagtagt cacgctcgtt gaatcgccgc
     7141 gtctcggtcc gcagcatgcc gccgcacttg atgctgatcg tgatggactt catggtggtg
     7201 ctcctttcgt tactcgggct ctcgtccctc tcgactgttg cagccgacag gggggtgaac
     7261 cggaactcca ggacaagcca ctcgatgaaa cacacgaaac agatggcgag tcggtcggag
     7321 agcgaaatct tcatttcaga agcgtgctcg gcggggtgcg accgaaccgg aaccgaaccg
     7381 acacgaacgc gagagcgtgc gtcggagcgt caggacgtcg aacgaagagg agaggagcca
     7441 cacctcgaac atgcctgccg agacggcagc gaaacggaag tgcagcggcc tacgtgacgc
     7501 cggctccgtg ctatgtttcg cgacggttct ctgacaaccg agcggtgaca gaaaagtgcc
     7561 agaaactccg tggaacggcg ctgggggacc tcggggatgt acgggggcaa ccggccgtcc
     7621 cgttcatccc cagtgagtta cggatgtccc ggggacgact ggggatagga gcaactggtt
     7681 cgtaatcagt aggtccccag ttcaagtctg ggtttcggct ccactcttca tgcgggtttg
     7741 agcgcaaggc gcgttcgccc gcccagttcc cgccgagata caggccccct cgcccgtcac
     7801 tccccgtccc gcggaatgtc gaccggctgc gcgagggtcc attcgaccac cgccgggatc
     7861 tcggcgggcg gaacgccctt ccagtagatc gtccagtcgg agagtcgcac gaggccgtcg
     7921 gcgagcttcg cgtaccagtc gccgatcggg ttcgacgggc gggcgcgcca gaagacggcg
     7981 gggctctcgg cggcgagggc gtcccagagg tcgcgcgcga tcccctcgcc ctgcgcctcg
     8041 gcggagacgg cgaacttcgt cagataggcg ccgagaggcg tcgcgacgag gacggcgcag
     8101 ccgcggtagc tctcctcgac gtagacgcgg ccgggcgtcc gcccgaagaa ggcatcggag
     8161 ggggggcggc gaaagctcga ggcgaggagc gcgcggagtc gagagaggtc gacccccgcc
     8221 cagccatcgt gcctctcgat ccgcgcgccg cggcggagga gcgtccccgc ccccttcacg
     8281 gtgaagagct cgcggaggag gttcagcggg gaggtcacgg cgacgctgaa cgacggcgga
     8341 gcggattcct cgcagaggcg ccgggcgtgc gcgaggatga gcgactcctt gcgggagagc
     8401 tcgccgctcg cgaggaggcc cgccgcctcc gtcgtgaggt tcacgatcgg gacgagaagg
     8461 cctccctgcc ggagcccgcc ggggcggtgg aggaagagga gcttccgcgc gccgagctcg
     8521 cgaaggagga cgccgaggcg gtcgagccgg ccgtcggcgt cctcgccttc gacgggcggg
     8581 aagacgagga ccgggacgcc ggccccgcgg gcacaggcgg ccaggcgttc gacgacgcgc
     8641 tccgagccgg ccggcacgac ctcgtgacgg acaccttccg cctcgagccg tcggcagagg
     8701 ctcgccgcgt gtcgcgccgc gtcggcgggc tggaagaggc cgaggacgac ggtggggaag
     8761 agctcgaggg ccgcgaggaa gcggagatcc tggacgacgc cgctcgccgc gtccgccacg
     8821 acgtgcgcgt cgatggcgat cgccgcgaac tgctcccgag ggcgggcccg gaagaggtcg
     8881 acgtagaggc gcgcctccga cgggcggccg gtgctctcga ggaagcggag gacggcctcc
     8941 tgcggggcga gctcggcgcg gctcgaggcc tcgggcgtct cggtgctcat ggggcggcga
     9001 ggatacggcg attcggctgc gccgcggcgg acccggaaga ggaggcctgc aggcggcttc
     9061 ccgccctcag aaccggtacg tcgcggcgcc gacggtgacg ccggagccga acgccaggag
     9121 cagtgcccgc ctcccggggg cgagcgcccc ttccgaccgc gcgcggtcga gagcgaggac
     9181 gaacgaggtc gtgagggtgt cggggagcgt cgccgtgaag tcggcgacct tctctctcgg
     9241 aacgccgagg acggccggga gccgcgagag gaacgcgggc gaaacctgcg cggggacgac
     9301 gaggtcgacg tcggcggggg tgaggccgtc gcgctcgaaa gcctcggtcg cggcggcagc
     9361 cgccccctcg agccaggcct cctcgagctc ggcccggcgg cggatgagga gcttcccgtg
     9421 cggctccttc aggctgacga cagacgtata gaggtcggcc cgctcctccc gcgtgtcgaa
     9481 ggcgaacgcg ccgaagcccg tgccgctcgt cggggagagg tcgaggagca tcgcggcgcc
     9541 gctcgcgggg atcgtcgact ccgcgtcggg gcgccggtcg gagttcgcct cgccggcgac
     9601 gaccatcccg acggcgacct ccccggcgag catctgcgcc gagacgacgt gcgcggcgct
     9661 cagcatcccg acgttgccgt tcaggaggtc gaaggagagg gtccgccggc cgtggaagtc
     9721 gacgttgatg tcgagggcgt gctggacgta gcaggcgatc gccggctcgc agacgtgccc
     9781 gtcgcggtgg acgccggcgt tcacgaggac gcggacgtcg gccgggcggt atttcgacgc
     9841 ggcgaggcac ctcttcccgg cctcgaccgt gtgcttcagc gcgccccacc ggaggaggcc
     9901 gcgccggggc gggctgaccc cgaggctctc gatccgggcg cgcctcaagc cggagctccg
     9961 ttcacgtggg cgcggtagcg gtcggggagg tcgtcgaacg tgaaggtcgc gtgggagatg
    10021 acgatcccgg aggcgccgct cacgaggagg acgttctgcc cggagcggat cgtcccgcgg
    10081 aggatgaagt cgtggagggc gagggcgtgg ctcgcggtcg tcgtgttgcc gaaggtctcc
    10141 gcgtgagaga ggaagacgtc ggggagatca caccccatgt agcgcgagat ggcccggatc
    10201 ccctgccgga tcgcgcgcgt cgagacctgg tgggggatga cgtggtggac gtcgctccag
    10261 tcccagcccg tcgcgtcgag cgcctgcttg aggtaggcgg ggaagttccg gttcccctcg
    10321 atctggaggc cgcgcgcctt cgtgacgagg atgcccccgc tcccgcggga ggagggtttc
    10381 gaccagcagt agtggtcgtg gcgcgccccg gagacgaggt cgagccagtg gaagccgacc
    10441 gcctcgtcgg gagaggcgtc gacgatcacg gccgcgccgc agtcgccgag ggtcagcgcg
    10501 gcgagctgcc cgtcgaggct gtggcgcact tcgcgcgccg ccgtccgggc gaggggcatg
    10561 ttctgctcgc cgctcacgac catcccgcac cggatcgcgc ccgagcggat gagcccctgg
    10621 aggacccaga tgccgttcag gaggccggcg caggcgttca cgacgtcgaa gacgagcgcc
    10681 ttcttcgcgc cgacggcccg gcggacgagg acggccgtcg ccggctcgaa cgtgaactcg
    10741 tcgggcctgt tgtgcttcga gatgctcgtg cagacgatcg cgtcgagctc ctccggcgcg
    10801 tagcgcgaca tcgcgagggc gcggcgcgcg gcgccgaccg ccaggtcgcc cgcgtactcg
    10861 tcgacggcga cccggcgcgt gacgatgccc gtgatccgct cgaggtcgag gcggggacgc
    10921 cgccggcagg aggccatcag ctcctcggtc gtcaccacct tctcggggag acgtaccccg
    10981 aggctctcga tcctgatctt tcgggtcatc cctcgcccgt cggtgatggt accgcgggac
    11041 gcctcgccgt cagcggaaga gctccgccgc cccctcgcgg aaggagacag cgggcgcgta
    11101 gccgagctcc cggcgggcct tcgccaggtc gtagcgggcg tggcacgcca cgaagcgggc
    11161 cccgtagcgc gtgagcggcg gcgggcggcg aagacgagcc gcccgggccg tgccttcgac
    11221 gagcgcggcg acggcgagga cgacggggaa ggggatcgag aggcgggggc gggggacgcc
    11281 gagggcgtcg gcgatgccgt cgaccgcctc gcgcgcggtg atctcctcgt cgtcggtcag
    11341 gtggaagatc tctccggctg cggcctcggc ctcgcccgcg aggaggaggc cccggacgag
    11401 gttttcgacg tggacgagtc cgacgaggtt ccgcccgccg ccgacgtatg gcatcgcgcc
    11461 ccgccggagg agacggacga ttcgcgggac gacgttcggg tcgcccgggc cccagatcgc
    11521 cccgggccg
//
LOCUS       DAOTEZ010000089        11492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3955_length_11492_cov_6.074582, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000089 DAOTEZ010000000
VERSION     DAOTEZ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11492
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3955_length_11492_cov_6.074582"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="P5164_06605"
     CDS             complement(<1..425)
                     /locus_tag="P5164_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43602.1"
                     /translation="MRRRKSWQEDLIPVAVAVLAQVAMLRFVDWANANVATQPIARQW
                     YDHRFYYAPQLLGILIVLLLLRAFRSFLQEQGVRVRDFLRSSVVVLLLAILVVVAVHL
                     DQLVGLLGRFVPAAADAARYLRRAPAYELLSRYSLARLGV"
     gene            484..1074
                     /locus_tag="P5164_06610"
     CDS             484..1074
                     /locus_tag="P5164_06610"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRY43603.1"
                     /translation="MKHSFPSGALLGLAAALLSAPASAQSWSDPGIGYGARLGLGQAR
                     SADAPTAEGALHVRYRLTGSLGLEGSLGLRRETVGDGDGPLLRLTEVPFTGTGQLFFF
                     PRGRFQPYLLAGAGLHVVRAEPEGRNPASGAKTEALFAVHAGAGLDVRPSRTSAVHLD
                     ARWTFLEPTTIGDLEKAGFDVAPGYWAVALGVTFYR"
     gene            complement(1078..2055)
                     /locus_tag="P5164_06615"
     CDS             complement(1078..2055)
                     /locus_tag="P5164_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020117055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRY43604.1"
                     /translation="MTTLPVTLYDVRAAAARIAPHVHRTPVLTSRTGDAACGARLFLK
                     CETFQRVGAFKARGAFSRLTLLSADERRRGVVAFSSGNHAQAVALAARDLGVPATIVM
                     PDDAPALKLAATRGYGAEVILYDRRNEDREAIARRLVEERSVTLVPPFDDEAVIAGQG
                     TAALELLAEVPDLDVVVAPCGGAGLLAGTAVAATALRPGIRVFGVEPEAGDDVARSLR
                     EGRRVAIPVPETIADSLQTTRVGERNFAILQALVEGVLTVSDLELRRAMAFLFSRMKL
                     VVEPGGSAAAAALLAGRVPGVAGRRVGVVLSGGNVDAVRFGELVAGLAG"
     gene            complement(2052..2744)
                     /locus_tag="P5164_06620"
     CDS             complement(2052..2744)
                     /locus_tag="P5164_06620"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRY43605.1"
                     /translation="MRAVLLGFPLFLLIALWRATLRIRLVGREHRDAIERSGRPVLHA
                     IWHQRMVAGIFRFPYEGVVTMASRSRDGDVIAGFLFWWGYRVVRGSSSRGGSEALAEM
                     VDALKGTTRGAALTPDGPRGPARRCKTGILRLAEAVDAPIMPVGASSTRPRFLRSWDR
                     YLVPMPFSRCAVVFGEGLRRAAGESDESFLARVDAAIDAMTDEADRLCGVVGAPRERG
                     PKAPAQTKEAKA"
     gene            2801..3418
                     /locus_tag="P5164_06625"
     CDS             2801..3418
                     /locus_tag="P5164_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY43606.1"
                     /translation="MRLVAENVSRAFGPRRIFGPLSFATEPGRVLGVAGPNGSGKTTL
                     VKVLAGLLRPSEGSVRLEGPAGARLSVHEAAGAIGWCAPDLSLYGELTPAENLEFFAR
                     VAGRPRARDEVDGRLASVGLDPRRLRSIRTRFLSTGQRQRLKLAYSVLSDPAVLLLDE
                     PGSNLDEAGHRAVAAIVAGQRERGSVVLATNDPAELALADERVTL"
     gene            3415..4092
                     /locus_tag="P5164_06630"
     CDS             3415..4092
                     /locus_tag="P5164_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HRY43607.1"
                     /translation="MILRAAAAVFVKDLRTEWRTRVAINALLLFAFSVLVLVGYAVGP
                     TRLTLEDRPVVNSVLLWIVLFFSAMTGLSRAFVNEEETGTAAALRLSAPPAAVFLGKL
                     LGNLALLGVVMAVVVPLFLALMSFGIAAPGLFLAVLALGGVGIGAASTFIAAIVSKTA
                     AKGALFAVLATPLLLPPLVAAVTGTQIAATQPDLVAGLDAVRLLFAYDGLMVTASFFL
                     FDAVWRE"
     assembly_gap    4356..4455
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4456..5033
                     /locus_tag="P5164_06635"
     CDS             <4456..5033
                     /locus_tag="P5164_06635"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HRY43608.1"
                     /translation="SSRIVFFHVPTAWISALAFLLAALHSGLYLKTRKMERDDAAAAA
                     ARLGVLFCVLATVTGSLFAKVMWNSYWNWDPRQTSIVLLLLVYGAYLGLRQAIEEPER
                     RATLSAAYALIAFVSVPFLMFAVPRMYASLHPDTVINARGRIEMSPDIKLVFFSSMIA
                     FTALFAWMYALDVKATRLVRRLSERAGGFEA"
     gene            5030..5191
                     /locus_tag="P5164_06640"
     CDS             5030..5191
                     /locus_tag="P5164_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HRY43609.1"
                     /translation="MTPDAPTVAVSHGALFYVAAVNIIIWAGLLAYLVHLDRKVRAAI
                     ARQSSEDPS"
     gene            5188..5595
                     /locus_tag="P5164_06645"
     CDS             5188..5595
                     /locus_tag="P5164_06645"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HRY43610.1"
                     /translation="MKKAYWAGAALIVAFLLLGLTTFSSSMTPYVTFDEALSSPRVCQ
                     VMGGLEKGSSKYDTTTQTLRFNLVDLESKKVLPVTYRDVKPANFEEAVSIVAIGKYQG
                     EAFHAEKLLVKCPSKYQGEETEKHYGAKVSVKS"
     gene            5595..7958
                     /gene="ccsA"
                     /locus_tag="P5164_06650"
     CDS             5595..7958
                     /gene="ccsA"
                     /locus_tag="P5164_06650"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRY43611.1"
                     /translation="MPALGYAPGTLALWFALASGIATIVAYARALGLQKEGGPAYEST
                     LLLGRRLYYAHAAAILVASAILMSRLLANDFRLSYVTSYSSRELPLRYLVSTFWAGQE
                     GSFLTWLFFGAIIGFFVVRTAREQEPPVMAVYVLSFLGIVAILVKQSPFRFLAEVPPD
                     GQGLNPLLQDPWMVIHPPVMFAGFASLSVPFAFAIGALWTKRWDGWVVRAMPWALFTF
                     VCLGTAILMGGYWAYKTLGWGGYWAWDPVENTSLVPWLATTALVHGMYLQKTREKHRK
                     INVILAILAFCCILYGTFLTRSGVLADFSVHSFVDLGITGWLVAIILVFLFGGLALLA
                     YRWREIPVVNDIDPATGKEKEEPFLSRSVLFILAVTLFCASGLVILLGTSAPILTRIG
                     GKASQVATSFYDVTHTPLAIVMALLIALVPFVSWRGETWAGVGKKVALPALLGVLGVV
                     LFLFLGVRKPVDLLFLGAAVFGLASSAEILVLFVRRKAIASAGGYLAHVGTAIMLVGI
                     LISGAYEVKTQVTLHRNRPLVIEQHGRPISFTFTGLYFVNDDGVVKTQSQLDMTKLAD
                     RRAKQAMEVTVSDGKATWKAYPKLYTNEKSGQLMANPDVRKSFFFDLYVSPQAYEPGA
                     PPRAEGALAVLRKGEEKTVHGVNVRFLEFSTEGAHTEGGAFTIGTKLAVTSAGATAEV
                     TPKFVVTLGGGEGQRFENPLSPLPGTDSGQVRIRRVSPNDAAVELELLGLDPAGDRKP
                     AVPESFSIDVTTKPLIALVWAGFYVMMAGGLVSMIKRAKDARQAAIA"
     gene            complement(8371..9189)
                     /locus_tag="P5164_06655"
     CDS             complement(8371..9189)
                     /locus_tag="P5164_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43612.1"
                     /translation="MISLLVLLAALVPGAADQTLPRFTLPPQYVERDPLWVSVDEAFS
                     PDGDLREGVFSRSARHTMPHQVVVSAGPGEPRKPGSDCLGGIASTGLPFSGQDRTLLD
                     LTRGASRIAAGTIVAATPGFLEGLAGTLLELRVDESLKGRFSATSIFVFYPYVYHRSG
                     RVTVCRSDLRYNLRPGAGSRALFFSFYPSRDVEDTLLEHRPNHLMLEGADGSLETEPA
                     FRDPAAPHAFGDVVEHVRGLIRIPQEPDPVPSLRKDSDADPIRPPCRPKAARRR"
     gene            complement(9603..10655)
                     /locus_tag="P5164_06660"
     CDS             complement(9603..10655)
                     /locus_tag="P5164_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011712372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRY43613.1"
                     /translation="MSNLNEDAVLEALRKVKYPGFSRDIVSFGFVKDVAVGGGNVSFR
                     LSMTTASAEIAEQIRTEADAALRALPGVTAVTIKVETGQAPVNLAGAQGARPDVLPET
                     RFTVAVASGKGGVGKSTVAANLALALRQLGYTVGLLDCDMYGPSQQMMMGIDEKPFLN
                     DEEKIVPIERFGVRVMSLGFLMDVDTPVIWRGPMIYKAIEQFLGDVAWGKQDFLIVDL
                     PPGTGDAQLTLTQKVALSGAVIVSTPQDVALIDARKGLAMFQKVNVPVLGLIENMSGF
                     ECPKCGHVEPIFKTGGGERTARELNVPFLGRVPLDPRVALSGDAGMPIVAAEPDSAST
                     KAFLELGQAVAKAVGA"
     gene            complement(10736..11449)
                     /locus_tag="P5164_06665"
     CDS             complement(10736..11449)
                     /locus_tag="P5164_06665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRY43614.1"
                     /translation="MQLLYTAWVWLFGLLVTPVFGSLAILTSWIPPRGRVYQYWARWW
                     SRTLLAGAGIDVSVETSEAAKGVPEAIFMANHTSAADIVVLFVALARDVRFVAKKQLF
                     WIPFLGWSMWLAGFIPVDRENKEKAREAFDQIGKRLKKGASVLVFPEGTRSRNGKLGA
                     FKKAGFLLAMRTGLPIVPVGISGARAVLGADGLLVRRGRIEVRVGDPIPSDGYTFARR
                     AEYVEKVRAEILRLGDSAE"
BASE COUNT         1570 a         4281 c         3813 g         1728 t
ORIGIN      
        1 accccgagac gggcgaggct gtagcgcgag aggagctcgt aggccggcgc ccggcggagg
       61 tatcgcgcgg cgtcggcggc cgccgggacg aaccgcccca gcaggccgac gagctggtcg
      121 aggtggaccg cgacgacgac gaggatcgcg aggaggagga cgacgacgga cgaccggagg
      181 aagtcgcgca ctcgcacgcc ctgctcctgc aggaagctcc ggaacgcgcg cagaaggagc
      241 aggacgatca ggatcccgag cagctgaggc gcgtagtaga agcggtggtc gtaccactgc
      301 cgggcgatcg gctgggtggc gacgttcgcg ttcgcccagt cgacgaaccg gagcatcgcc
      361 acctgggcga gcaccgccac ggccaccggg atcaggtcct cctgccagct cttccttctt
      421 ctcacgttcg gcgattatcg acgatcgccc tccggacggt ccgtgccgtt accatgggcc
      481 gccgtgaagc actcctttcc atccggcgcg ctcctcgggc tcgcggccgc gctcctctcc
      541 gccccggcgt ccgcgcagtc gtggtccgat cccggaatcg gctacggggc ccggctcggg
      601 ctcgggcagg cgcgctccgc cgacgccccg acggccgaag gcgccctcca cgtccgctat
      661 cgcctgacgg gctcgctcgg cctcgagggc tccctcggcc tccgccgcga gacggtcgga
      721 gacggcgacg gtcccctcct ccgcctgacg gaggtgccct tcaccggcac cgggcagctc
      781 ttcttctttc cacgcgggcg tttccagccg tatctcctcg cgggcgcggg cctccacgtg
      841 gtccgagccg agccggaggg gaggaatccg gcttccggcg cgaagaccga ggccctcttc
      901 gccgtccacg cgggagcggg gctcgacgtc cgcccttcgc ggacgagcgc cgtccacctc
      961 gacgcacgct ggaccttcct cgagccgacc acgatcggcg acctcgagaa ggcgggattc
     1021 gacgtcgcgc ccggctactg ggccgtcgcg ctcggcgtca cgttctaccg ctgagcctca
     1081 gccggccaga ccagcgacca gctcgccgaa ccgcacggcg tcgacgttcc cacccgagag
     1141 gacgacgccg acgcgcctcc cggcgacgcc cgggacccgt cccgcgagga gcgccgccgc
     1201 ggcggccgag cctcccggct ccacgacgag cttcatccgc gagaagagga acgccatcgc
     1261 gcggcgcagc tcgaggtcgc tcacggtgag gaccccttcg acaagcgcct gaaggatcgc
     1321 gaagttccgc tcgccgacgc gcgtcgtctg gagcgagtcg gcgatcgtct cggggaccgg
     1381 gatcgccacg cgacgccctt cccggagcga gcgcgcgacg tcgtcgcccg cctccggctc
     1441 gactccgaag acgcggatcc cgggccggag cgccgtcgcg gcgaccgccg tcccggcgag
     1501 aagtccggct ccgccgcagg gcgcgaccac gacgtcgagg tcgggcacct cggccaggag
     1561 ctcgagcgcg gccgtcccct gccccgcgat gacagcctcg tcgtcgaacg gcgggacgag
     1621 cgtcacgctc cgctcctcga cgagccgccg ggcgatcgcc tctcgatcct cgttccgacg
     1681 gtcgtagagg atcacctcgg cgccgtagcc cctcgtcgcg gcgagcttga gggccggcgc
     1741 gtcgtcgggc atgacgatcg tcgccgggac gccgaggtcg cgcgcggcga gcgccacggc
     1801 ctgggcgtgg ttccccgagg agaaggcgac gacgccccgc ctccgctcgt cggcggaaag
     1861 gagcgtcagc cgcgaaaagg cgccgcgcgc cttgaacgcc ccgaccctct ggaacgtctc
     1921 gcacttgagg aagagccgtg ccccgcaggc ggcgtcgccc gtccgcgacg tcagcaccgg
     1981 ggtccggtgg acgtgcggcg cgatccgcgc cgcggccgcc ctcacgtcgt agagagtgac
     2041 cgggagcgtc gtcacgcctt cgcctccttc gtctgcgcgg gagccttcgg cccccgctcg
     2101 cgcggcgcgc cgacgacgcc gcagagccgg tcggcctcgt cggtcatcgc gtcgatcgcc
     2161 gcgtcgacgc gcgcgaggaa cgattcgtcg ctctcccccg ccgcgcgccg gagcccctcg
     2221 ccgaagacga cggcgcagcg cgagaagggc atcggaacga gatagcggtc ccaggagcgg
     2281 aggaaacgcg gccgggtcga cgacgcgccg accggcatga tcggggcgtc cacggcctcg
     2341 gccaggcgga ggatcccggt cttgcagcga cgcgccgggc cgcgcggacc gtcgggggtg
     2401 agcgccgccc cgcgcgtcgt ccccttcaga gcgtcgacca tctcggcgag cgcctcgctc
     2461 ccgccgcgcg acgacgagcc gcggacgacg cgatagcccc accagaagag gaagccggcg
     2521 atgacgtcgc cgtcgcggct cctcgacgcc atcgtcacga ccccctcgta cgggaagcgg
     2581 aagatccccg cgaccatccg ctggtgccag atcgcgtgaa ggaccggccg tcccgaacgc
     2641 tcgatcgcgt cgcggtgctc gcggccgacg aggcggatcc ggagcgtcgc tctccagagg
     2701 gcgatcagga ggaagagagg aaagccgagg aggacggcgc gcacgacccg attctccacg
     2761 acgcgcgccc cccagcgccc tccctgtatc ctccccgccc gtgcgcctcg tcgccgagaa
     2821 cgtctcccgc gccttcgggc cgcgccggat cttcggcccg ctctccttcg cgacggagcc
     2881 tgggagggtc ctcggcgtcg ccgggccgaa cggctcggga aagacgacgc tcgtgaaggt
     2941 cctcgccggc ctcctccgcc cctccgaagg aagcgtccgg ctcgaaggcc cggccggcgc
     3001 gcggctctcc gtgcacgagg ccgccggcgc gatcggctgg tgcgcgccgg acctctccct
     3061 ctacggcgag ctgaccccgg ccgagaacct cgagttcttc gcccgggtgg cggggcgccc
     3121 gcgagcgcgc gacgaggtcg acgggcgcct cgcttccgtc ggcctcgacc cgaggcgcct
     3181 ccgctcgatc cggacgcggt tcctctccac gggccagcgg cagcgcctca agctcgccta
     3241 ctcggtcctc tccgaccccg ccgtcctcct cctcgacgag cccggctcga acctcgacga
     3301 ggcggggcac cgcgccgtcg cggcgatcgt cgccggacag cgggagcgcg gcagcgtcgt
     3361 cctcgccacg aacgaccccg ccgagctcgc cctggccgac gagcgggtga cgctgtgatc
     3421 ctgcgcgcgg cggcggccgt cttcgtgaag gacctccgga ccgagtggcg gacgcgggtc
     3481 gcgatcaacg cgctgctcct cttcgcgttc tcggtcctcg tcctcgtcgg ctacgccgtc
     3541 ggcccgacgc gcctcacgct cgaggaccgt ccggtcgtca actcggtcct cctctggatc
     3601 gtcctcttct tctcggcgat gacggggctc tcccgcgcct tcgtgaacga ggaggagacc
     3661 ggaacggccg cggcgctccg cctctcggcc ccgccggccg cggtcttcct cggcaagctc
     3721 ctcgggaacc tcgcgctcct cggcgtcgtc atggccgtcg tcgtcccgct cttcctcgcg
     3781 ctcatgtcgt tcgggatcgc cgcccccggc ctcttcctcg cggtcctcgc gctcggcggc
     3841 gtcggcatcg gcgcggcctc caccttcatc gcggcgatcg tctccaagac cgcggcgaag
     3901 ggggccctct tcgcggtcct cgcgaccccg ctcctcctcc cgcccctcgt cgccgccgtc
     3961 accgggaccc agatcgccgc cacccagccc gacctcgtcg ccgggctcga cgccgtccgc
     4021 ctcctcttcg cctacgacgg cctgatggtg acggcctcgt tcttcctctt cgacgccgtc
     4081 tggcgcgagt agccatcccc cttcgaggcg aatcaagcgc ccgttcggcg agggtttgga
     4141 atccagcccc gaaaaggtcc ggtatgagcc tcccctcccg tatcatcccg gccgatgcag
     4201 gttctcggaa ccgccgggaa ggtcgtcctg ggcctcggga tggcggtcgt cctctgggcc
     4261 gccttcctct atgcccaccc cgcggagagc ttcgtgggcg agtcttcccg catcgtcttc
     4321 ttccacgtcc cgacggcctg gatctccgcc ctggcnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnagtct tcccgcatcg tcttcttcca cgtcccgacg gcctggatct
     4501 ccgccctggc gttcctcctg gccgccctcc actccggcct ctacctgaag acccgaaaga
     4561 tggagcggga cgacgcggcg gccgccgccg cgcggctcgg ggtcctcttc tgcgtcctgg
     4621 ccaccgtcac cggctccctc ttcgcgaagg tgatgtggaa ctcgtactgg aactgggacc
     4681 cgaggcagac ctcgatcgtc ctcctcctcc tcgtctacgg ggcctacctc ggcctccgcc
     4741 aggcgatcga ggagccggag cggcgggcga cgctctcggc ggcctacgcc ctgatcgcct
     4801 tcgtctccgt gccgttcctg atgttcgccg tcccgcgcat gtacgcctcg ctccaccccg
     4861 acaccgtgat caacgcgcgg ggccggatcg agatgtcccc cgacatcaag ctcgtcttct
     4921 tctcctcgat gatcgcgttc accgcgctct tcgcctggat gtacgccctc gacgtgaagg
     4981 cgacccggct cgtccggcgc ctctcggaga gggccggagg gttcgaagca tgacgcccga
     5041 cgccccgacc gtcgccgtct cgcacggcgc gctcttctac gtcgccgccg tgaacatcat
     5101 catctgggcg gggctcctcg cctacctcgt ccacctcgac cggaaggtcc gcgcggcgat
     5161 cgcgcggcag tcctcggagg acccgtcgtg aagaaagcct actgggccgg cgccgccctc
     5221 atcgtcgcct tcctcctcct cggcctgacg accttctcct cctccatgac gccctacgtc
     5281 acgttcgacg aggcgctctc ctcgccgcgc gtctgccagg tcatgggcgg cctcgagaag
     5341 ggctcctcga agtacgacac gacgacgcag acgctccggt tcaacctcgt cgacctcgag
     5401 tcgaagaagg tcctcccggt cacctaccgc gacgtcaagc cggcgaactt cgaggaggcc
     5461 gtctccatcg tcgcgatcgg gaagtaccag ggcgaggcgt tccacgccga gaagctcctc
     5521 gtgaagtgcc cctcgaagta ccagggcgag gagacggaga agcactacgg cgccaaggtc
     5581 tcggtgaagt cctgatgccg gccctcggct acgccccggg gacgctcgcg ctctggttcg
     5641 cgctcgcgtc cgggatcgcg acgatcgtcg cctacgcgcg ggccctcggg ctccagaagg
     5701 agggaggccc ggcgtacgag tcgaccctcc tcctcgggcg gcgcctctac tacgcccacg
     5761 ccgcggcgat cctcgtcgcc tcggcgattc tcatgtcgcg gctcctcgcg aacgacttcc
     5821 gcctttccta cgtcacgtcg tattcctctc gcgagctccc cctccgctac ctcgtctcga
     5881 cgttctgggc cggccaggag ggctcgttcc tcacctggct cttcttcggc gcgatcatcg
     5941 ggttcttcgt cgtccgcacc gcccgggagc aggagccccc cgtgatggcg gtctacgtcc
     6001 tctccttcct cggcatcgtc gcgatcctcg tcaagcagtc cccgttccgc ttcctcgccg
     6061 aggtcccgcc cgacggacag gggctgaacc cgctccttca ggacccgtgg atggtcattc
     6121 acccgccggt catgttcgcc ggcttcgcgt cgctctcggt gccgttcgcg ttcgccatcg
     6181 gggcgctctg gacgaaacgc tgggacgggt gggtcgtccg ggcgatgccc tgggcgctct
     6241 tcacgttcgt ctgcctcggg acggcgatcc tgatgggcgg ctactgggcc tacaagacgc
     6301 tcggctgggg cggctactgg gcctgggacc cggtcgagaa cacctcgctc gtcccgtggc
     6361 tcgcgacgac ggccctcgtc cacgggatgt acctgcagaa gacgcgcgag aagcaccgga
     6421 agatcaacgt catcctcgcg atcctcgcct tctgctgcat cctctacggc acgttcctca
     6481 cccgctccgg ggtcctcgcc gacttctcgg tccactcctt cgtcgacctc gggatcaccg
     6541 gctggctcgt cgcgatcatc ctcgtcttcc tcttcggcgg cctcgcgctc ctcgcctatc
     6601 gctggaggga gatcccggtc gtcaacgaca tcgaccccgc caccggcaag gagaaagagg
     6661 agccgttcct ctcgcgctcc gtcctcttca tcctcgcggt cacccttttc tgcgcctcgg
     6721 gcctcgtcat cctcctcggc acgtcggccc cgatcctgac ccggatcggc ggcaaggcct
     6781 cgcaggtggc gacgagcttc tacgacgtca cccacacccc gctcgccatc gtcatggcgc
     6841 tcctcatcgc cctcgtgccg ttcgtctcct ggcgcggcga gacgtgggcg ggcgtcggga
     6901 agaaggtcgc cctcccggcg ctcctcggcg tcctcggcgt cgtcctcttc ctcttcctcg
     6961 gcgtgaggaa gccggtcgac ctcctcttcc tcggcgccgc ggtcttcggc ctcgcctcct
     7021 ccgccgagat cctcgtcctc ttcgtgaggc gcaaggcgat cgcgagcgcg ggcggctatc
     7081 tcgcccacgt cggcacggcg atcatgctcg tcggcatcct gatctcgggc gcctacgagg
     7141 tgaagaccca ggtgacgctc caccggaacc gcccgctcgt gatcgagcag cacggccgcc
     7201 cgatcagctt cacgttcacc ggcctctact tcgtcaacga cgacggcgtc gtgaagacgc
     7261 agtcccagct cgacatgacg aagctcgccg accgccgcgc caagcaggcg atggaggtga
     7321 ccgtcagcga cggcaaggcc acctggaagg cctacccgaa gctctacacg aacgagaagt
     7381 cgggccagct catggcgaac cccgacgtga ggaagtcgtt cttcttcgac ctctacgtct
     7441 cgccccaggc ctacgagccc ggcgccccgc cccgcgcgga gggagcgctc gcggtcctcc
     7501 ggaagggcga ggagaagacg gtccacggcg tgaacgtccg cttcctcgag ttctcgacgg
     7561 agggcgccca caccgagggc ggcgccttca cgatcgggac gaagctcgcc gtcacctccg
     7621 ccggcgcgac cgcggaggtc accccgaagt tcgtcgtcac gctcggaggc ggcgagggcc
     7681 agcgcttcga gaatcccctc tcgcccctcc cgggaacgga cagcggacag gtccgcattc
     7741 gccgcgtctc cccgaacgac gccgccgtcg agctcgagct cctcggcctc gacccggccg
     7801 gcgaccggaa gcccgccgtc cccgagtcgt tctccatcga cgtcacgacg aagcccctca
     7861 tcgccctcgt ctgggccggc ttctacgtga tgatggccgg aggcctcgtc tccatgatca
     7921 agcgcgcgaa ggacgcgagg caggccgcga tcgcgtgagc ccggggcggg gacgagaatc
     7981 ccggaaagaa ccccgacggg gaccgtccgc caattcgacg acggcccggc agagacgccg
     8041 ggccgtttcc gttggtggga aggctctcgt caacgatcca ggggcgcggg ggaggggtgc
     8101 gtcccgccgg cgccctctgc gcgcgcgcga cccgcatcgg ccccgctgcg cgggccgccg
     8161 gcgagcgcgc aagctcgtcg ctgcgctcct cgaatcggtg cgcgctcttc ccccggcggc
     8221 ctgctcgcgg agccgtgcgg ggccccgcgc gtgctcgaac ggtcgcctcg cggggcgcac
     8281 ccctccctcg cgccgtggca gcttcgacgt ctgcggccgc cctgcgggcg gcacgtagag
     8341 tgtagatggt agacctgacc cccaggcgtg tcagcgtctt cgagcggcct tcggacggca
     8401 cggcggacga atcggatcgg cgtcggagtc cttgcgaagc gaggggaccg ggtccggctc
     8461 ctgcgggatg cgaatcagac ctctcacgtg ctccacgacg tctccgaagg cgtggggagc
     8521 cgcgggatcc cggaaggccg gctccgtctc gagcgagccg tccgcaccct cgagcatcag
     8581 gtggttcggg cggtgctcga ggagcgtgtc ctcgacgtcc cgcgaggggt agaagctgaa
     8641 gaagagcgcc cgactcccgg ctccgggccg gaggttgtat cggagatccg acctgcagac
     8701 cgtcacgcgc ccgctgcggt ggtagacgta ggggtagaag acgaagatcg aagtggccga
     8761 gaagcgcccc ttcaggctct cgtcgaccct cagttcgagc agcgtccccg cgagcccctc
     8821 gaggaatccg ggagtggccg cgacgatcgt gcccgcagcg attcgcgacg cgccgcgcgt
     8881 caggtcgaga agtgtgcggt cctgccctga gaaggggaga cccgtactgg ctatccctcc
     8941 caggcagtca gaccccggct tccgaggctc accgggcccg gcggagacga cgacctggtg
     9001 cggcatcgtg tgtcgcgcgc tgcgggagaa gacgccctcg cgaaggtccc catccgggct
     9061 gaaggcctcg tcgacggaga cccagagcgg atcccgctcg acgtactgcg gcgggagcgt
     9121 gaacctcgga agggtctgat ccgcagctcc gggcacgagc gcggccagga ggaccaacag
     9181 ggagatcatc ggttcgcttc tcctcctccg acgcgtgtcg ggctcgtcgc gccgatcgta
     9241 accgcccgac tcggcaacgg agacggtggc tggcacttgt caaactcgct gtctcgtctg
     9301 gcgagggcag ctctcctgtg tgcggcctcc cgctccggtg ggccgagccc cgggggtggg
     9361 tcccgcgagc cgactcgagc ccggccgggg acccgctcgc tgtcacgagg accgggcggg
     9421 gatcaggccc gcgcgctgtt tgaggagcga agcgacgagt tcgcgcgggc cccctgcccg
     9481 gccgcagcga aagcgcacgc gggtcggccg ggagagcccg aggccgtggg acccaccccc
     9541 ggggtgaggc tcccctctcc cgtgccctcg tgctccggtg cccccgtgcc ccggggatct
     9601 ggttacgccc cgacggcctt cgcgacggcc tggccgagct cgaggaaggc cttcgtcgag
     9661 gcggagtcgg gctcggcggc gacgatcggc attccggcgt cgccggagag ggcgacgcgc
     9721 gggtcgagcg ggacgcggcc gaggaacggg acgttcagct cccgggccgt ccgctcgccg
     9781 ccgcccgttt tgaagatcgg ctcgacgtgg ccgcacttcg ggcactcgaa gccgctcatg
     9841 ttctcgatca ggccgaggac ggggacgttc accttctgga acatcgcgag ccccttgcgg
     9901 gcgtcgatga gggcgacgtc ctgcggggtc gagacgatga cggcgcccga gagggcgacc
     9961 ttctgcgtca gcgtcagctg cgcgtcgccc gtcccgggcg ggaggtcgac gatgaggaag
    10021 tcctgcttcc cccacgccac gtcgccgagg aactgctcga tcgccttgta gatcatcggc
    10081 ccgcgccaga tgaccggcgt gtcgacgtcc atcaggaagc cgagcgacat gacgcggacg
    10141 ccgaagcgct cgatcgggac gatcttctcc tcgtcgttga ggaacggctt ctcgtcgatc
    10201 cccatcatca tctgctgcga cgggccgtac atgtcgcagt cgaggaggcc gaccgtgtag
    10261 ccgagctgcc ggagcgcgag ggcgaggttc gcggcgacgg tcgacttgcc gacgcccccc
    10321 ttccccgagg cgacggccac cgtgaagcgc gtctcgggga ggacgtcggg gcgcgccccc
    10381 tgcgctcccg cgaggttcac cggcgcctgc cccgtctcca ccttgatcgt caccgccgtg
    10441 acgcccggga gcgcccggag cgccgcgtcg gcctccgtcc ggatctgctc cgcgatctcc
    10501 gccgaggcgg tcgtcatcga gaggcggaac gagacgttcc ctccgccgac ggcgacgtcc
    10561 ttcacgaagc cgaaggagac gatgtcccgg gaaaagccgg ggtacttgac cttccggagc
    10621 gcttcgagga cggcgtcctc gttcaggttc gacacgggat acctccgatc gtggtcgccg
    10681 gcgcgcgtgg caggcgctcg cggcccgtcg cccggcgccg cgcggcgggc ggatcttact
    10741 ccgccgagtc gccgagccgg aggatctcgg cccgtacctt ctcgacgtac tccgccctgc
    10801 gggcgaaggt gtagccgtcc gaggggatcg ggtcgccgac tctcacctcg atgcggcccc
    10861 ggcggacgag gaggccgtcg gcgccgagga cggcgcgggc gccggagatg ccgaccggga
    10921 cgatggggag ccccgtccgc atcgcgagga ggaaccccgc cttcttgaac gcaccgagct
    10981 tcccgttccg cgagcgggtc ccttcgggaa agacgaggac cgaagccccc ttcttcaggc
    11041 gtttcccgat ctggtcgaac gcctcgcgcg ccttctcctt gttctcgcgg tcgacgggga
    11101 tgaagccggc gagccacatc gaccagccga ggaaggggat ccagaagagc tgcttcttcg
    11161 ccacgaagcg gacgtccctc gcgagcgcga cgaagaggac gacgatgtcg gccgccgagg
    11221 tgtggttcgc catgaagatc gcctcgggga cacccttggc cgcttcggac gtctccacac
    11281 tcacgtcgat cccggccccg gccaggagcg tccgggacca ccagcgggcc cagtactggt
    11341 agacgcggcc ccgcggcggg atccacgagg tgaggatggc gaggctgccg aacacgggag
    11401 tgacgaggag cccgaagagc cacacccacg ccgtgtagag gagctgcatc ggagcggaag
    11461 tctaccgggc cgcccgtaca atcgcccgcg tg
//
LOCUS       DAOTEZ010000090        11485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3957_length_11485_cov_5.946194, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000090 DAOTEZ010000000
VERSION     DAOTEZ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11485
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3957_length_11485_cov_5.946194"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..683)
                     /locus_tag="P5164_06670"
     CDS             complement(27..683)
                     /locus_tag="P5164_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HRY43615.1"
                     /translation="MPRSRQAGKPPRDFWDLTTVEVARALLGCRLVRRRPGRPPVTVV
                     LVETEAYLGVLDRAAHTWNGRRTARVAPMWGPAGHAYVFRVYGMHDCLNVVTREEGVP
                     EAVLLRAAVPEAWWSGLPVPRDELLAASGPGRLCRALGVGRELSGVSLRGPELELLLP
                     LPGEERPLLVGPRVGVDYAGEAASWPLRFAVAGCAAVTRRAGLAVEPRGGRRWRTGRA
                     "
     gene            706..1680
                     /locus_tag="P5164_06675"
     CDS             706..1680
                     /locus_tag="P5164_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRY43616.1"
                     /translation="MTKECPSPAPGCPVLVTGGAGFIGSHVVDALLALGHRVVVVDDL
                     STGDLRRVPAEVRFVRGDVRDPGLDALLVEERIDTVFHLAAQVDVRKSVENPVLDAEV
                     NVLGTIRVALAASKAGVAQVVFSSSGGAIYGDPVGERADEDHPLNPCSAYGVAKLAGE
                     KYLAALAPDGGHLLTVLRYANVYGPRQDGRGEAGVVGIFMNRLLSGQDAVIHGDGLQT
                     RDFVHVADVVAANQASFRRRVGGTMNVGTGIETSVRELYGLVAAACHAERSPRFGPGK
                     PGEQRRSVLDVARDRERLGLAELVPLEGGMHATAEWFRLHRAGQGLGG"
     gene            complement(1704..2960)
                     /locus_tag="P5164_06680"
     CDS             complement(1704..2960)
                     /locus_tag="P5164_06680"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HRY43617.1"
                     /translation="MDYRSLLKNVEKTLSAIEESEDVVSTVASVGETVVGNFRNELGI
                     IGGRLYQLEDGEYVLRRGFGRSKKVSEGLTVPASYEPIRRAIEEGVVLADLKDPWVDR
                     ELEARLGVTRFAAVSFGDDRFILSFDVHPRVAADDLLVSLGIIRTVLDSKVKSEQLED
                     LLEEARRIQQSILPRHFPEMGDYEVWAATEPAEAVGGDFYDFITLGPDAFGTAIADAS
                     GHGLVAALLVRDVYVGLRMGIKGDLKIARAIERLNRILNKSRLSTKFVSLFYGELEAN
                     GEIIYVNAGHPGPIHLHARTRSFSEMESTGMVLGPSPNAVYGRRAAKMEPGDLLLLYT
                     DGIVEAHDLRGREFGLDRVRRILLDYRKRSARETVEKLMSTVRQFSAGRAAEDDRTVV
                     VIRRTGRPRGAQPEGPTSPAASPSAD"
     gene            complement(3000..4535)
                     /gene="dacB"
                     /locus_tag="P5164_06685"
     CDS             complement(3000..4535)
                     /gene="dacB"
                     /locus_tag="P5164_06685"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HRY43618.1"
                     /translation="MAFAGSKRPLIRAVPFVLLILAFIASRADGDSTRSKRRPPRKAA
                     VSEPARPLTLEERLRAAARRTPVRNRAEVAVAIAPVGEAPILEMNAGTELILASTTKL
                     FTTAAALDRLGPGYRFRTSLLQDGELLPDGTLAGRLVVRGGGDPAISGRLYDDDPWAV
                     FRPWAAALAARGVRSVRDGLALDTSFFDDVTTHPDWPAAQEQHWWQAPVSALSYNDNV
                     VLVRVTGGLRPGGRASLGFYPAQPFLMSLVGRVTTHRGRGVSVGVSRNPGSPRVVASG
                     AVGRRRTWSGDVTVVDPPLYLASAFTKVLEDEGIAVGGEPEVRTERRSVPMSLPLLHV
                     HETPILPALAVCNKRSQSFFAEQILKTLGAERRGLGSWANGLAEVSEFLRSLGLDPAR
                     YRLADGSGRSRENRASAAAYLDFLQALATRWPHFAAFEPTLAVTGDMAGSLRYRMLGP
                     DTRGKVFAKTGNIAGVVTLAGWIEARSGQRYAFVILANGGCGEGSGHAYQDRILATLA
                     RFG"
     gene            4574..7159
                     /locus_tag="P5164_06690"
     CDS             4574..7159
                     /locus_tag="P5164_06690"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HRY43619.1"
                     /translation="MPIRVDLATRTVTLSPGALLAPTARRIGDGRGGSMERRWIGQAV
                     HRRVLSEALASIPGYAVEKAVRHSFAVDGFTAILEGRLDGRFEDPDGTVVVDEVKSVH
                     FAEELPRLAGSPRMDRFARQLQWYLLAIARSEGRPVRGRLVLADIETGSTKLIPVEWD
                     EAEVAEDLHARVRALLEAFFDDLALAEAKASEADALTFPFSRFRAGQREMAAAVARAV
                     RQGEQLLVEAPTGIGKTAAALFPMLVEALRRGRKLYVLTSKTTQQEIFRKTLEAISAA
                     SASSVRLRAKERMCANGVVLCHEDHCAFAKDYAAKLEASGLVDRLLATAPHLEPDLVF
                     EEARSEMVCPFEVSLELAERADVVLGDYNYAFDPVVALSGLRDPASLDGALLLVDEVH
                     NLVERGRAIWSPEISEAEVRRLARAIGTTSPRTAAAAQEAAEEVLALVLDAASGLPAA
                     PDPWNPSVDLVPLDLDRLDDIRLSMESLLVRHLADLRNGGERVPDDPVLALYFLYAGF
                     HDVARRAAPSGRLVEAFDLLASRGPDGARLALLCKDPSRLLGETFDAAFATVGMSATL
                     QPPEFYRDLLGLDPDRTSVLSLPSPFPKENRAVLVAPGVDTTWKRRAAAMPRIAALVA
                     EIADACPGNVLALFPSYRFVDDVRALLPPLPGHRVLRPSDRSTELERNGVLSALREEG
                     SRILLLAVSGGPFAEGVDYPGAMLHGVVVVSPALPQVRFEQERMRLYFEERFGRGFEY
                     AYVIPGMTRVVQSAGRVIRSETDVGVIALVCRRFLQKPYRTFLPTHWYDEAPDELSSR
                     EPGEAVRTFFAGAALGSASESDPARLGPATTVTAGPPPPGRRRTPSRRPRRPSGLPGP
                     SGDPG"
     gene            complement(7156..8115)
                     /locus_tag="P5164_06695"
     CDS             complement(7156..8115)
                     /locus_tag="P5164_06695"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRY43620.1"
                     /translation="MLRRLAAKVGAGRGRTGVDGADSVAEVWGEQAGTWSIGGVRHWT
                     ELARVQAILNRRVSGDEDVDPYLWFLRTRLEGRLPVARALTIGCGAGDLERGLATYGF
                     AASHDAFDVAPGAIEKARTAASGAGLGRVRYSVANGNDLTLESNVYDVVFGVHSIHHI
                     ERLEHLFEQIARSLKPDGLFFMNEFVGPTRFQWSDRQLEVVNGLLRALPARYRRSTVG
                     DGLKERFPRPTVAEMVASDPSEAVRSSEILELAEAHFELLEVRPYGGTVLHLLLHDIA
                     GNFSATSPGGPALLEAIADLEWALVSSGDLPSDFAVVVARPRR"
     gene            complement(8252..9934)
                     /locus_tag="P5164_06700"
     CDS             complement(8252..9934)
                     /locus_tag="P5164_06700"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRY43621.1"
                     /translation="MKLNALVLPVTAAVLLTLGAPAFAQCPEEGCPAQTLGTIEAPAE
                     GALVSGFVRVTGFALNGNLVSNVDLFIDGTDEANRVTPAGGANINLPRPDVTQWLPQY
                     AGTPGEQPGFEMSFRAANYANGTHTLHVRITDVTGCCYFLAPRTIRIDNAKNQAPFGV
                     LDVPLPDSSVHANGVLAVAGWALDDRRIDHVDVLIDGLIERGAVLGINRPDVAAFYPN
                     VPSAATSGFLVFIDSTRLTNGVHTLTVKATDDQGQQGLIDVRRFQVFNNAPNLPPFGD
                     VDQPMLQATWFGNCLRLPGGPSGGEIRDTRYVMFVSGWALDTSVYAERGGVSHVFLEM
                     DGVVLKDTRGAFGTLGCRREFNLDNALVDCYGFYRPDVNVFYPGFPQSANSGYFFAVD
                     VGHLLTEKGFKEGNHMLQVKAADKEDNVTLLKELPVHIECATQNLDPSPLGYVSDPMN
                     YQFVGGIHPVLGWALDLDGVIRVRVLIDGVPQIDAVTGRDYAEYGLPSPDVSAVYPNY
                     PQRANARWRFYLDTTKLTNSEHDLLVEVIDGRGNYRSAGTRRFVVNNNTLVR"
     gene            10185..11243
                     /locus_tag="P5164_06705"
     CDS             10185..11243
                     /locus_tag="P5164_06705"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRY43622.1"
                     /translation="MPKAASNALRAALSLGLAALLLWLFFRSLDLAELGRALSGARPA
                     GLAVALAITVVNVPLRAWRWIRLLRHVERVSLRESFVATTIGFAASVLLPARAGEIVR
                     PAVLSRRTGLPFAPALASIAVERLIDLVTVILLFVAYAAGGWAPAGISPEAQARMELL
                     RRAALVVGAVALAVFAGLGLLAARPALAERFLAPLERRLPKRFAARIVSLLRSFLGGL
                     AAIRTGRDVAVLAASSLVMWLLNALQFHVVAGAFGIQLAFPISFFVMTWAVLGLAIPT
                     PGGVGGYHAAVAYALTGFYAVAAAPAAATALATHAIAFVPITVAGAFLVAGSGLTFRR
                     LATSGPSGPDSSGSSLRP"
     gene            11240..>11485
                     /locus_tag="P5164_06710"
     CDS             11240..>11485
                     /locus_tag="P5164_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HRY43623.1"
                     /translation="MKCPFCGGVSDRVVDSRESREGEVIRRRRECLSCQRRFTSYERV
                     EESPLVVVKSDGRREEFDRAKVLRGLRRACEKRPVEEA"
BASE COUNT         1530 a         4299 c         3997 g         1659 t
ORIGIN      
        1 gacggggaag aaccgggtcg tccgcttcac gcgcgtcccg tccgccaccg acgacctcct
       61 cgcggctcca cggcgagccc cgcgcgacgc gtgacggccg cacagcccgc caccgcgaag
      121 cggagcggcc acgaggccgc ctcgcccgcg tagtcgacgc cgaccctcgg cccgacgagg
      181 agcggacgct cctcccccgg gagcggcagg aggagctcga gctccggtcc gcggagagag
      241 acaccggaga gctcccgacc gacgccgagc gcccggcaga ggcggcccgg accggacgcg
      301 gcgaggagct cgtcccgggg aacgggcagt cccgaccacc aggcctcggg gacggccgcc
      361 cgcaggagga ccgcttccgg cactccttcc tcgcgcgtca cgacgttcag gcagtcgtgc
      421 attccgtaga cccggaagac ataggcgtgc cccgccggcc cccacatcgg cgcgacgcgc
      481 gccgtccgcc ggccgttcca ggtgtgcgcg gcgcggtcga ggacgcccag gtacgcctcg
      541 gtctccacga ggacgaccgt caccggggga cggcccggcc gacgccgcac gagccggcac
      601 ccgaggaggg cgcgcgcgac ctcgacggtc gtaaggtccc agaagtcccg gggcggcttc
      661 ccggcttgtc ggctccgcgg cacgccgcta ttctcgcgga cgagcatgac aaaagagtgt
      721 ccttctcccg cgcccggctg cccggtcctc gtcacgggcg gcgccggctt catcggatcg
      781 cacgtcgtcg acgcgctcct ggccctcggt caccgcgtcg tcgtggtcga cgacctctcc
      841 accggcgacc tccggcgcgt gccggccgag gtccgcttcg tccggggcga cgtccgcgat
      901 ccgggcctcg acgccctcct cgtcgaggag aggatcgaca cggtcttcca cctcgccgcc
      961 caggtcgacg tgaggaagtc ggtggagaac ccggtcctcg acgccgaggt gaacgtcctc
     1021 ggcacgatcc gcgtcgcgct cgccgcctcg aaggcaggcg tcgcccaggt cgtcttctcc
     1081 tcgagcggcg gcgcgatcta cggcgacccc gtcggcgagc gcgccgacga ggaccacccc
     1141 ctgaacccgt gctcggccta cggcgtcgcc aagctcgccg gcgagaagta tctcgccgcg
     1201 ctcgcgccgg acggtggaca cctcctgacg gtcctccgct acgcgaacgt ctacggcccg
     1261 cgccaggacg gccgcgggga ggcgggcgtc gtcgggatct tcatgaaccg cctcctgtcc
     1321 gggcaggacg ccgtcatcca cggcgacggc ctgcagactc gggacttcgt ccacgtcgcc
     1381 gacgtcgtcg ccgcgaacca ggcctcgttc cgccggcgcg tcggcgggac gatgaacgtc
     1441 ggcaccggga tcgagacgag cgtgagagag ctctacggac tcgtcgcggc cgcgtgccac
     1501 gccgagcggt ccccgcgctt cggtcccggc aagccgggcg agcagcgccg gagcgtcctc
     1561 gacgtggcgc gcgaccgcga acggctcggg ctcgccgagc tcgtccctct cgagggcggg
     1621 atgcacgcga cggcggagtg gttccgcttg caccgggcgg gccagggcct cggcggttga
     1681 ggcgcctctc cgcggcgtcc ccgtcagtcg gctgacgggg acgcggccgg ggaggtcggc
     1741 ccttccggct gcgccccgcg cggacggccc gtccggcgga tgacgacgac ggtccggtcg
     1801 tcttccgcgg cccggcccgc gctgaactgc cggaccgtcg acatcagctt ctcgaccgtc
     1861 tcccgggccg agcgcttccg gtagtcgagg aggatccgcc ggacccggtc gagcccgaac
     1921 tcccgtccgc gaaggtcgtg ggcctcgacg atcccgtcgg tgtagaggag gagcagatcg
     1981 cccggctcca tcttcgcggc cctccggccg tagacggcgt tcggcgacgg accgaggacc
     2041 atcccggtcg actccatctc cgagaagctc cgcgtccggg cgtggaggtg gatcggcccg
     2101 gggtggccgg cattgacgta gatgatctcg ccgttcgcct cgagctcgcc gtagaagagc
     2161 gagacgaact tcgtcgagag ccggctcttg ttcaggatcc ggttgaggcg ctcgatcgcg
     2221 cgcgcgatct tcaggtcgcc cttgatcccc atccggaggc ccacgtagac gtcgcggacg
     2281 aggagcgccg cgacgaggcc gtgccccgac gcgtcggcga tcgccgtccc gaacgcgtcg
     2341 ggtccgagcg tgatgaagtc gtagaagtcc ccgccgaccg cctcggccgg ctccgtcgcg
     2401 gcccagacct cgtagtcccc catctcgggg aaatggcgcg gaaggatgga ctgctggatc
     2461 cgccgcgcct cctcgaggag gtcctcgagc tgctcgctct tgaccttcga gtccaggacg
     2521 gtccggatga tcccgaggga gacgagcagg tcgtcggccg ccacgcgcgg gtggacgtcg
     2581 aaggagagga tgaaccggtc gtcgccgaac gagaccgccg cgaagcgcgt gacgccgagc
     2641 cgcgcctcga gctcgcggtc gacccagggg tccttcaggt cggccaggac gaccccttcc
     2701 tcgatcgcac gccggatcgg ctcgtacgag gccgggaccg tcagcccctc cgacaccttc
     2761 ttcgagcggc cgaacccgcg gcggaggacg tactcgccgt cctcgagctg gtagagccgg
     2821 ccgccgatga tcccgagctc gtttcggaag ttcccgacga cggtctctcc caccgaggcg
     2881 acggtggaga cgacgtcctc gctttcctcg atcgcggaaa gggtcttctc gacgttcttc
     2941 aggagggagc gatagtccat cggggccgcg cagtatacgt cgcgggcgcg gagaggttct
     3001 cagccgaacc gcgcgagcgt cgcgaggatc cggtcctggt aggcgtgccc gctcccctcg
     3061 ccgcatccgc cgttcgcgag gatgacgaac gcgtagcgct gcccgctccg cgcctcgatc
     3121 cagccggcga gcgtcacgac gccggcgatg ttgcccgtct tcgcgaagac cttcccgcgc
     3181 gtgtcggggc cgagcatacg gtaccggagc gagcccgcca tgtctccggt gacggcgagc
     3241 gtcggctcga acgccgcgaa atggggccag cgcgtcgcga gagcctgcag gaagtcgagg
     3301 taggcggcgg cgctcgcgcg gttctcgcgc gagcgtcccg agccgtccgc gagccggtag
     3361 cgcgccgggt cgagcccgag cgaccggagg aactccgaca cctcggccag cccgttcgcc
     3421 cacgagccga gtccccggcg ctcggcgccg agcgtcttca ggatctgctc ggcgaagaac
     3481 gactgcgacc tcttgttgca gacggcgagc gccggaagga tcggcgtctc gtggacgtgc
     3541 aggagaggga gcgacatcgg gaccgaccgc cgctccgtcc tcacctcggg ctcgccgccg
     3601 acggcgatcc cctcgtcctc caggactttc gtgaacgccg aagcgaggta gagcggcggg
     3661 tcgacgaccg tgacgtcgcc cgaccaggtc cgtcgccggc cgacggcgcc cgacgcgacg
     3721 acgcgcgggc tccccgggtt gcgcgagacg ccgacgctca cgccgcggcc ccggtgcgtc
     3781 gtcacccggc ccacgagcga catgaggaac ggctgcgccg ggtagaagcc gaggctcgcc
     3841 cgcccgccgg ggcggagacc gcccgtgacc ctcacgagaa cgacgttgtc gttgtacgac
     3901 agcgccgaca ccggggcctg ccaccagtgc tgctcctgcg cggccggcca gtcaggatga
     3961 gtcgtgacgt cgtcgaagaa cgaggtgtcc agcgcgaggc cgtccctcac ggagcggact
     4021 ccgcgcgccg cgagcgccgc ggcccagggg cggaagacgg cccaggggtc gtcgtcgtag
     4081 agccggcccg agatcgcggg gtctccgccg ccccggacga cgagccggcc cgcgagcgtg
     4141 ccgtcgggaa ggagctcccc gtcctgcagg agagaggtcc ggaaacggta gcccggcccg
     4201 agccggtcga gcgccgccgc cgtcgtgaag agcttcgtcg tcgacgccag gatcagctcc
     4261 gtcccggcgt tcatctcgag gatcggcgcc tcgcccaccg gcgcgatcgc caccgcgacc
     4321 tccgcgcggt tccggacggg cgtccggcgc gcggcggccc gcaggcgctc ctcgagcgtg
     4381 agcggccgcg cgggctcgga gacggccgcc tttcggggcg ggcgccgctt cgaacgggtg
     4441 gaatccccgt ccgcccggct cgcgatgaaa gccaggatca gcagaacgaa cggcacggcc
     4501 cggatgaggg gccgcttcga cccggcgaaa gccaccgccc cgattgtagg cgacctcgtc
     4561 cgtagaatgc cgcgtgccga ttcgggtcga cctcgccacg agaaccgtca ccctctcgcc
     4621 gggcgcgctc ctcgccccga ccgcgaggcg gatcggcgac gggcggggcg gctcgatgga
     4681 gcggcgctgg atcggccagg ccgtccaccg ccgcgtcctt tccgaggcgc tcgcttccat
     4741 tcccggatac gccgtcgaga aggccgtccg ccactcgttc gccgtcgacg ggttcaccgc
     4801 catcctggag ggacggctcg acggccgctt cgaggacccg gacggcaccg tcgtcgtcga
     4861 cgaggtgaag tcggtccact tcgccgagga gctcccgcgg ctcgccggct cgccgcgcat
     4921 ggaccgcttc gcccgccagc tccagtggta cctcctggcc atcgcccgat ccgaagggcg
     4981 cccggtccgg ggccggctcg tcctggccga catcgagacg ggaagcacga agctgatccc
     5041 cgtcgagtgg gacgaggccg aggtggcgga ggacctccac gcgcgcgtcc gcgccctcct
     5101 cgaggccttc ttcgacgacc tcgctctcgc cgaggccaag gcgtccgaag cggacgccct
     5161 cacgttcccc ttcagccggt tccgggccgg tcagcgcgag atggcggccg ccgtggcccg
     5221 cgccgtccga cagggcgagc agctcctcgt ggaggcgccg accggcatcg ggaagacggc
     5281 cgccgccctc ttcccgatgc tcgtcgaggc cctccggcgc ggacggaagc tctacgtcct
     5341 cacgtcgaag acgacgcagc aggagatctt ccgcaagacg ctcgaggcga tctccgccgc
     5401 ctcggccagc tccgtccgcc tccgtgcgaa ggagaggatg tgcgcgaacg gcgtcgtcct
     5461 ctgccacgag gaccactgcg ccttcgcgaa ggactacgcc gcgaagctgg aggcctccgg
     5521 cctcgtggac cgcctcctcg ccaccgctcc gcacctcgag cccgacctcg tcttcgagga
     5581 ggcgcgctcg gagatggtct gtccgttcga ggtctccctc gagctcgccg agcgggccga
     5641 cgtcgtcctc ggcgactaca actacgcgtt cgacccggtc gtcgccctct ccgggctccg
     5701 cgaccccgcc tccctcgacg gcgcgctcct cctcgtcgac gaggtccaca acctcgtcga
     5761 gcgcggacgc gccatctggt cgccggagat ctccgaggcc gaggtccggc gcctcgcccg
     5821 cgcgatcggg acgacgagcc cgcggacggc cgccgcggcg caggaggccg ccgaggaggt
     5881 cctcgccctc gtcctcgacg cggcgtccgg cctccccgcg gcgcccgacc cctggaaccc
     5941 ctccgtcgac ctcgtccccc tcgacctgga ccgcctcgac gacatccggc tgtcgatgga
     6001 gtcgctcctc gtccggcacc tcgccgacct ccggaacggc ggcgagcgcg tccccgacga
     6061 cccggtcctc gcgctctact tcctctacgc cggcttccac gacgtcgcgc gccgggccgc
     6121 cccgagcggg cgcctcgtgg aggccttcga cctcctcgcc tcccgcggtc cggacggcgc
     6181 ccgcctcgcg ctcctctgca aggacccgtc gcgcctcctc ggcgagacgt tcgacgccgc
     6241 cttcgccacc gtcgggatgt cggccacgct ccagccgccc gagttctacc gcgacctcct
     6301 cgggctggac cccgaccgca cctccgtcct ctcgctcccc tcccccttcc cgaaggagaa
     6361 ccgcgccgtc ctcgtcgctc cgggagtcga cacgacctgg aagaggcgcg ccgccgcgat
     6421 gccccgcatc gccgcgctcg tcgcggagat cgccgacgcc tgccccggga atgtcctcgc
     6481 cctcttcccc tcctaccgct tcgtcgacga cgtccgcgcc ctcctcccgc ctctccccgg
     6541 ccaccgcgtc ctccggccgt ccgaccgctc caccgagctc gagcggaacg gggtcctctc
     6601 cgcgctccgc gaggaggggt cgcgcatcct cctcctcgcc gtctcgggcg gaccgttcgc
     6661 ggagggcgtc gactacccgg gcgcgatgct ccacggcgtc gtcgtcgtct cccccgcgct
     6721 cccgcaggtc cgcttcgagc aggagcggat gcgcctctac ttcgaagagc gcttcgggcg
     6781 cggcttcgag tacgcctacg tcatccccgg gatgactcgc gtcgtacaga gcgcgggccg
     6841 cgtcatccgc tcggagaccg acgtcggagt gatcgccctc gtctgccgcc gcttcctcca
     6901 gaagccctac cggaccttcc tcccgacgca ctggtacgac gaggcccccg acgagctctc
     6961 ctcgcgcgag ccgggcgagg cggtgaggac attcttcgcc ggcgcggccc tcggatcggc
     7021 gtcggagagc gacccggcac gcctcgggcc cgctacgacg gtgaccgccg ggcccccgcc
     7081 tccgggccgc cgcagaaccc cgtcgaggag gccccgtcgg cccagcggcc tgccggggcc
     7141 ctcgggcgat cccggctagc gtctcggacg cgcgacgacg accgcgaagt cgctcggcag
     7201 gtctccagat gacacgagag cccattcgag gtcggcgatc gcctcgagca gcgccgggcc
     7261 gccaggactc gtcgcggaga agttgccggc gatgtcgtga agcaggaggt ggaggaccgt
     7321 tccgccgtac ggcctcacct cgaggagctc aaagtgagcc tccgccagct ccaggatctc
     7381 gctcgatcgg acggcttcgg acgggtccga agcgaccatc tcggcgacgg tcgggcgcgg
     7441 aaagcgttcc ttcaggccgt ctcccacggt gcttcgccgg taccgggccg gcagggcgcg
     7501 aagaagaccg ttcacgacct cgagctgccg atcggaccac tggaatcgcg tcggcccgac
     7561 gaattcgttc atgaagaaga ggccgtccgg cttcagcgac cgggcgatct gctcgaagag
     7621 atgctcgagc cgctcgatgt ggtggatcga gtggacgccg aagacgacgt cgtagacgtt
     7681 cgactccagc gtcaggtcgt ttccgttcgc cacggagtag cgcacgcgtc cgaggcccgc
     7741 cccggacgcc gccgtccgcg ccttctcgat cgcgcccggg gcgacgtcga atgcgtcgtg
     7801 ggatgcggcg aaaccgtacg tcgcgagacc tcgctcgagg tcgccagctc cgcacccgat
     7861 cgtgagcgcc cgagccacgg ggaggcggcc ctcgaggcgc gtccggagga accagaggta
     7921 ggggtccacg tcctcgtcgc ctgagacgcg ccggttcagg atcgcctgaa cccgggcgag
     7981 ctcggtccag tgtcggacac cgccgatact ccaggtcccc gcctgctcgc cccagacctc
     8041 cgccacggaa tccgctccgt cgacgccggt ccggcccctc cccgcgccca ccttcgcggc
     8101 caggcgccgg agcacgccgc gaagagtcac gcgagcttcc tgcgtctctc gagacgatcc
     8161 gaatcggcca tggcgatatc cgtcctccct tacagacgaa acggccccgc gctcgcgcgc
     8221 ggggccgccc gtcgtggatc ggctttcggg ttcagcggac gagcgtgttg ttgttgacga
     8281 cgaagcggcg cgtcccggcg gaccggtagt tgccgcggcc gtcgatgacc tcgacgagga
     8341 ggtcgtgctc ggagttcgtg agcttcgtcg tgtcgaggta gaagcgccaa cgggcgttcg
     8401 cgcgctgcgg gtagttcggg tagaccgccg agacgtcggg gctcgggagg ccgtactccg
     8461 cgtagtcgcg cccggtcacg gcgtcgatct gcggcacgcc gtcgatcaga acgcgaaccc
     8521 ggatgacgcc gtcgaggtcg agcgcccagc cgaggaccgg gtggatcccg ccgacgaact
     8581 ggtagttcat cgggtcggag acgtagccga gcggcgacgg gtcgaggttc tgcgtggcgc
     8641 actcgatgtg gaccggcagc tccttcagga gggtgacgtt gtcctccttg tcggccgcct
     8701 tcacctggag catgtggttc ccctccttga accccttctc ggtcaggagg tggcccacgt
     8761 cgacggcgaa gaagtatccg gagttcgccg actgcgggaa gcccgggtag aagacgttca
     8821 cgtcggggcg gtagaagccg tagcagtcga cgagcgcgtt gtcgaggttg aactcgcgcc
     8881 ggcacccgag cgtgccgaag gcgccgcgcg tgtccttcag gacgacgccg tccatctcga
     8941 ggaagacgtg ggagacgccg ccgcgctcgg cgtagaccga ggtgtcgagc gcccagccgg
     9001 agacgaacat gacgtagcgg gtgtcgcgga tctcgccgcc ggacggcccg ccggggaggc
     9061 ggaggcagtt gccgaaccag gtcgcctgga gcatcggctg gtcgacgtcg ccgaacggcg
     9121 ggaggttcgg ggcgttgttg aagacctgga accgccggac gtcgatcaga ccctgctgtc
     9181 cctggtcgtc ggtcgccttg accgtgagcg tgtggacgcc gttcgtcagg cgggtcgagt
     9241 cgatgaagac gaggaagccg gaggtcgcgg cgctggggac gttcgggtag aaggccgcga
     9301 cgtcggggcg gttgatgccg agcaccgcgc cgcgctcgat cagaccgtcg atcaggacgt
     9361 cgacgtgatc gatccgccgg tcgtcgagcg cccagccggc gacggcgagg acgccgttcg
     9421 cgtggacgga cgagtcgggg agcgggacgt cgaggacccc gaacggcgcc tggttcttcg
     9481 cgttgtcgat ccggatcgtc cggggcgcga ggaagtagca gcagccggtg acgtccgtga
     9541 tccggacgtg gagcgtgtgc gttccgttgg cgtagttcgc cgcgcggaac gacatctcga
     9601 accccggctg ctcgcccggc gtccccgcgt actgcgggag ccactgcgtg acgtcgggac
     9661 gcgggaggtt gatgttggcg ccgcccgcgg gcgtgacccg gttcgcctcg tcggtcccgt
     9721 cgatgaagag atcgacgttc gagacgaggt tcccgttcag ggcgaagccc gtgacccgga
     9781 cgaagcccga gacgagggcc ccctccgccg gggcctcgat cgtcccgagg gtctgcgccg
     9841 ggcagccctc ctccgggcac tgcgcgaacg cgggcgcccc gagcgtcagc aggaccgccg
     9901 ccgtcaccgg aaggaccaga gcgttgagct tcatcagtct tcctccccag gctcacgagg
     9961 tctcaggttc acggtttccg gctccatcgc ggaatcagac ccggaacttt gagagcgtgc
    10021 gttcgtttgc gggaattcta ggtgggggcc aaaaccctgt caattcgagg gtttccccct
    10081 gttcgggcct tccccggccg cccggacggg ccggccaagg gtctcccttc cctcggaccc
    10141 ggtcccgccg cgtcggccgc cccctctcca agtaccatgc gccggtgccg aaggctgcct
    10201 cgaacgccct ccgggccgcc ctgtcgctcg gtctggcggc cctcctcctc tggctcttct
    10261 tccggagcct cgacctcgcc gagctcggac gggccctctc aggcgcccgc ccggccgggc
    10321 tcgccgtcgc cctcgcgatc acggtcgtga acgtgccgct ccgggcctgg cgctggatcc
    10381 gcctgctccg ccacgtggag cgggtctccc tccgggagtc cttcgtcgcc acgacgatcg
    10441 gcttcgcggc gtccgtcctc ctcccggccc gggcgggcga gatcgtccgg ccggccgtcc
    10501 tctcgcgccg gacggggctt cccttcgccc cggcgctcgc ctcgatcgcg gtcgagcggc
    10561 tgatcgacct cgtcaccgtg atcctcctgt tcgtcgccta cgccgcggga ggctgggcgc
    10621 cggccgggat ctccccggag gcgcaggccc ggatggagct gctgcgccgc gcggcgctcg
    10681 tcgtcggggc cgtcgcgctc gccgtcttcg ccgggctcgg cctcctcgcg gcccggcccg
    10741 ctctcgccga gcgcttcctc gcgccgctcg agcggcggct cccgaagagg ttcgcggccc
    10801 ggatcgtctc tctcctccgc tccttcctgg gcgggctcgc ggcgatccgg accgggcgcg
    10861 acgtggcggt cctggccgcc tcctcgctcg tgatgtggct cctgaacgcg ctccagttcc
    10921 acgtggtcgc cggggccttc ggcatccagc tcgcgttccc gatctccttc ttcgtgatga
    10981 cgtgggccgt cctcggcctc gcgatcccga cccccggggg cgtcgggggc taccacgcgg
    11041 cggtcgccta cgcgctgacc ggcttctacg ccgtcgccgc ggcgcccgcg gcggcgacgg
    11101 cgctcgcgac gcacgcgatc gcgttcgtcc cgatcacggt cgccggggcc ttcctcgtag
    11161 ccggcagcgg cctcacgttc cgccgcctcg ccacgtccgg cccctccggc ccggactcct
    11221 ccgggagtag tctccggccg tgaagtgccc gttctgcggc ggtgtttccg accgggtcgt
    11281 cgattcgcgc gagagccgcg agggcgaggt gatccgccga cggcgggagt gcctctcctg
    11341 ccagcgccgg ttcacgtcgt acgagcgggt cgaggagtct ccgctcgtgg tcgtcaagag
    11401 cgacggtcgc cgcgaggagt tcgaccgcgc gaaggtcctg cgcggcctcc gacgcgcctg
    11461 cgagaagcgc cccgtcgagg aggcc
//
LOCUS       DAOTEZ010000091        11442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3977_length_11442_cov_5.443576, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000091 DAOTEZ010000000
VERSION     DAOTEZ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11442
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3977_length_11442_cov_5.443576"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="P5164_06715"
     CDS             complement(<1..183)
                     /locus_tag="P5164_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRY43624.1"
                     /translation="MSSIADRLAVDLGLEPHPEGGRYRQLFRSGSVVAVPGRGIERAA
                     LTSILFLLARGEVSRWH"
     gene            complement(185..799)
                     /locus_tag="P5164_06720"
     CDS             complement(185..799)
                     /locus_tag="P5164_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017246682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY43625.1"
                     /translation="MTTDLAALFGGIDVYLFDQLLRGRIAPGMRVLDAGSGRGRNLVF
                     LLREGYEVFALDPNPTAVAAVRDLARRLAPALPETNVRCEPVDASTFPDACADVVISN
                     AVLHFALDETRFLDMLRGTWRLVAPDGLLFCRLASSIGIERLVRPLGGRRFALPDGTG
                     RFLVDEAYLAGLTEELGGRLLDPIKTTVVQGQRAMTTWVVRKGS"
     gene            complement(796..2055)
                     /locus_tag="P5164_06725"
     CDS             complement(796..2055)
                     /locus_tag="P5164_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY43626.1"
                     /translation="MTTSPHSRSGHFRGLGRLAAEATLGVTELVEAAHATIAAAPGPL
                     DPPPAGKTRGISRVVYGSVRGVTRLVGGGLDAALAGLAPALGALPPSPRREALVAALN
                     GVLGDHLEATGNPLAIRMRLRRHGEDLSFGRPGSPEGLPGSSRRVVLLVHGLCMNDAG
                     WSREGHDHGAALERDLGFEAVHVVYNSGRHVSSNGRSLAELLETFVGDAAAPPGEIAI
                     VTHSMGGLVVRSALVAGARAGHAWPALVRAVVFLGTPHHGAPAERLGHLAQAALGVSP
                     YARPFARLGRLRSAGITDLRYGNVLDDDWAGDDRFARHGDTRVPVPLPGGIAFFAVAG
                     TTATEPVAPGARIAGDGLVSVASALGHHRDPERTLALAPGRTWIAPGTDHLALLASRA
                     VYERVRQWLDDAGAGGVAAQASPGDRA"
     gene            complement(2101..3360)
                     /locus_tag="P5164_06730"
     CDS             complement(2101..3360)
                     /locus_tag="P5164_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43627.1"
                     /translation="MPLESDGGEGRHPAGLLSSPRRGFLLVFLLHVGFQGFFLTKLDV
                     RHVMPHTRFEVTAVAMSLYERGAFADPYGVPTGPTAHMPPFHPALLAGIYHLLGPNLA
                     AGYVWWFLLIASHGAMWGMLPWLAARLGLSAGGGLLAGLAGALLPRWPGYVEAPSAIA
                     IGLMLVAFLARWEGPRPSATGSLLLGAAVGAALHVSPSLLPVALGLLVWEALGAARRP
                     GLLRSALVAAGMLLACVPWTLRNERALGGLFFLRSNFGLELRMGNHEGAGANLDLSAR
                     GGTERHPRTDEGEARKVAELGELAYMRAAGREARAWIVSHPGDFLRLTGLRVLQFWLG
                     PVDDPVAMLGTGLLTVLSAVGAWRAFPSLPADRRAALLAPLLLFPLVYYLVGFEARYR
                     QPLDGLAFLLAACAFLPPRPLSTAAMG"
     gene            3638..8290
                     /locus_tag="P5164_06735"
     CDS             3638..8290
                     /locus_tag="P5164_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY43628.1"
                     /translation="MPSYARQFAGGFRRPDLLGAEGLGPAVAVAQDVSSSNPRSTVGT
                     LTEVHDLLRLLFARLGDAPAGLKPARGLFSWNGSGACPACRGLGLEDRLDPALLVADG
                     TKTIREGALRVSTPNGYLMYSQVTLAVLDDVLRAHGGSVDVPWRDLTDEVREVVLRGS
                     DRVLVPFGKHPLENRLKWKGITARPRQEGHYRGLLPVMEEILRGKRNDSILRFVRSTP
                     CAACGGTRLSKEALSVTWRGRTIAGLSGRTARELRAVFEEAGDGEVLGPIRDEVVARC
                     ALLEDLGLGYLSFDRAAPTLSPGEARRLRLLSLALGELRGLVVVLDEPSAGLHPHDTA
                     RLLGVLRRLTARGQTVVVVDHEPLLARNADFLVDLGPGPAKEGGRLLFAGAPADLLGP
                     DGPDTPTRRALHGEGAALPVAAREADEGEDVVLEGLARHNAAGATLRIRPRALNVVTG
                     VAGAGKTSLLHAAVARLRESGSFARIVAVDAEPIGRTPRSNAATYTGAFDVIRDLFAA
                     TPEAKARGFGRGHFTFNTAGGRCESCEGAGVHEVGMRWLGSVELVCGACGGRRFHADV
                     LAVTLDGLSVADVLDLTVAEAMERFRKEKKLARVLSALVDLGLGYLPLGQPATTLSGG
                     EAQRVKLATELAKATGPALFVLDEPTTGLHPADVAVLAAAFRHLCSARHTLLVADHDL
                     DLLRAADHVTEIGPGSGPEGGRVVTEGTSADLAACPESPTGAALRGEIGAALPSEAQA
                     GSRGDEPLALSGVTTHNLRGVAARFPCPGLTVVTGPSGSGKSSLVFDTLLAEAQARFS
                     DLLSPWARRFLPMNGGAELESASGLRAAVAVPADAARRNPRSTVGTTTGIDELLRLLF
                     ARGGARPCPVCGREAAGATCACGERLEPLLASDFSPHSERGACPSCRGLGFAPACDPA
                     RLVSRPDLPLDGGALDGTRYGAYLGEADGQHVATLRAAAAALGLDVSGPWSALSPEAR
                     ALAMDGAGERTFEVDWSWKRGKRSGVHRLAAKWAGIARLVEAEYERVHADERGEELEP
                     LLVDRPCTTCGGERLAPHSRAVTFAGKRAPETTDSESDSLVAWLDGIAARHAGVAPSL
                     LAATAPVREALAPRLRALCDAGLGYLSPAREMASLSGGEAQRVRLAAALGGGLVGVTY
                     VLDEPTQGLHPRDTERLVGVLRSLADAGNAVVVVEHDAALVAAADHVIELGPLAGPEG
                     GLVVADGPPAELRADPSTYTARLLARTDASPSRAPRALFPGVTVRGAHRHNLKDVDVD
                     FPAGGLVVVAGVSGSGKSTLVLEVLAPSLRAALRGDDRVGCRALERHAEIEDVLASDQ
                     EGLAVGGGSTVATLTGVGEELRRLLAATPEAKRRKLAATAFSTNVPGGRCESCAGRGV
                     VRVPMDLLPDVTVGCETCAGRRFSDDVLACRLDGRSITDLLDAPVSDATAWLADRPAL
                     AAPLAALRDLGLGYLRVGQEGTTLSSGERQRLRLARLLVAPRPDRVAVLLDEPTRGLA
                     LDDVDRLLSALGRLAAEGHLVVVVEHHPAVLAAADRVVELGPEGGERGGRIVRVRE"
     gene            8319..11192
                     /locus_tag="P5164_06740"
     CDS             8319..11192
                     /locus_tag="P5164_06740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRY43629.1"
                     /translation="MTDREQSTGSGTLEESESVLREERALYRDLVEAQPRGIYRIRSV
                     CPGAELPEGSLESGRARYVVEFVSSRFVQLLGMDPEEFHARHGAIDDLIHPDDRRSFT
                     EANERALTGQLPLHWEGRLTVRGAAAARWFRFESVPRALEAGGAVWTGTLEDVTEQRR
                     AIASLRESEALLRSFFEVPLFGACITSPTKDWLEVNDSLCRMLGYTREELVKLTWSEL
                     THPDDLAADVEQFDRVIRGEIDGYSLEKRYLRKDGSAVPVDLAVSCARFPDGAVDHLI
                     AIISDITERKRAEEQLRCHRERLEELVAERTVQLEKSRELLDATSRLARIGGWEYDVR
                     TERLTWTEMVREIHEVAPGYDLTVAAGLDFYAPESRPVIEPAFRRAVASGEPFDLELQ
                     LVTATGKRLWVRAIGHPTVEDGEVVRVIGVLQDIDARRRTEEDLARYRTRLEELVAER
                     TAEVEKANARLVEAQAVAHLASWEWDARNDEIRGSEELYRLFGVPPEGLARVEQLLDL
                     VHPDDRESVRIAIEEARTHGDGTLFEQSYRVLLPDGRLRFVNDRGVVSVDADGQLTGM
                     FGTCHDITSVREAEEALRESEERLRRIVSEAPFPIGLYAEDGEILLVNNAWRRITGYA
                     PEELRTIGEWAARAYGDRREIARADIESRFSLSDAIHEGEYAIRTKSGERRYWDFSSS
                     PVGRLPDGRRLVTTMAMDVTERRRAEEEVYAYREHLEELVRERTAELEAANREMATFS
                     YSVSHDLRAPLRALDGFSAILAQEYPDRLDERGRGYLDRIRAAARTMALLVDGLLSLA
                     QLNRQELTVAPVDLSALARKIGADLTSLEPGRVVRLIVPDGLTTDGDPRLLHVLLENL
                     IGNALKFTSLRPEATIELGRAERDGETVFFVKDNGVGFDMTYASKLFLPFQRLHSVNE
                     FPGTGIGLATVHRIVARHGGRIWPESSLDAGATFYFTLGAAEG"
     gene            complement(11167..>11442)
                     /locus_tag="P5164_06745"
     CDS             complement(11167..>11442)
                     /locus_tag="P5164_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY43630.1"
                     /translation="RRGDVHLVLLDMIMPRKGGREAATEIRRLRPDVKVLFSSGYSPE
                     LVGSREPALEGFELLMKPVTPLDLLRKVREVLDREAPGGVSLPRRRE"
BASE COUNT         1521 a         4348 c         4125 g         1448 t
ORIGIN      
        1 gtgccagcgg ctcacctctc cccgcgcgag gaggaagagg atcgacgtga gcgcagcgcg
       61 ctcgatgcct cgccccggca ccgccacgac cgatccggag cggaagagct gccggtagcg
      121 cccgccctcg gggtgcggct cgaggccgag gtcgacggcg aggcggtcgg cgatcgacga
      181 cacgtcagct ccccttccgg acgacccacg tcgtcatggc ccgctgcccc tggacgacgg
      241 tcgtcttgat cgggtcgagg agccgcccgc cgagctcctc cgtcaggccc gcgaggtacg
      301 cctcgtcgac gaggaagcgg ccggttccgt cgggaagcgc gaaccggcga ccgccgagcg
      361 gccggacgag ccgctcgatc ccgatcgacg aggcgaggcg gcagaagagg aggccgtccg
      421 gcgcgacgag tcgccaggta ccgcggagca tgtcgaggaa ccgcgtctcg tccaacgcga
      481 aatggaggac ggcgttcgag atcacgacgt cggcgcaggc gtcggggaac gtcgaagcgt
      541 cgaccggctc gcaccggacg ttcgtctccg ggagcgcggg cgcgagccgc cgcgcgaggt
      601 cgcgcacggc cgcgacggcc gtcgggttcg ggtcgagggc gaagacctcg tacccctcgc
      661 ggaggaggaa gacgaggttc cggcctcggc cgctgccggc gtcgaggacg cgcatccccg
      721 gtgcgatccg cccgcggagg agctggtcga agaggtagac gtcgatcccg ccgaagaggg
      781 ccgcgaggtc cgtcgtcatg cccggtctcc cggcgacgcc tgggcggcca cgccgccggc
      841 gcccgcgtcg tccagccact ggcggacgcg ctcgtagacc gcccggctcg cgaggagggc
      901 gaggtggtcg gtgcccggcg cgatccacgt ccggcccgga gcgagcgcca gcgtccgctc
      961 cgggtcgcgg tggtgtccga gggcgctcgc gacggagacg agcccgtccc cggcgatcct
     1021 cgcgccgggc gccacgggct ccgtcgcggt cgtcccggcg acggcgaaga acgcgattcc
     1081 gccggggagc ggcacgggga cccgcgtgtc cccgtgccgg gcgaagcggt cgtcccccgc
     1141 ccagtcgtcg tcgaggacgt ttccgtagcg caggtcggtg atccccgcgc tccggagccg
     1201 tccgagtcgc gcaaaggggc gtgcgtacgg gctgacgccg agggccgcct gcgcgaggtg
     1261 cccgaggcgc tccgccggcg cgccgtggtg gggcgtcccg aggaagacga ccgcccggac
     1321 gagggccggc caggcatgcc cggcccgtgc cccggccacg agcgcgctcc tcacgacgag
     1381 gccgcccatg ctgtgcgtca cgatcgcgat ctcccccggc ggagcggccg cgtcaccgac
     1441 gaacgtctcg aggagctccg cgagcgagcg gccgttggag gaaacgtgcc ggcccgagtt
     1501 gtagacgacg tggaccgcct cgaagccgag gtcgcgctcg agcgcggcac cgtggtcgtg
     1561 gccctcgcgg ctccacccgg cgtcgttcat gcacaggccg tggacgagga ggacgacccg
     1621 ccgggacgat ccggggagcc cctcggggct cccgggccgg ccgaacgaga ggtcctcccc
     1681 gtgccgccgc agccgcatcc ggatcgcgag cgggttcccg gtcgcttcca ggtggtctcc
     1741 gaggacgccg ttcagcgccg caacgagagc ctcgcgccgc ggcgacggcg gcagcgcacc
     1801 gagagcgggg gcgagacccg cgagggcggc gtcgagcccg cccccgacga gccgcgtcac
     1861 gccgcggacg ctcccgtaga cgacccggct gatcccgcgc gtcttccctg cgggcggcgg
     1921 gtcgagcggc cccggggccg ccgcgatcgt cgcgtgcgcc gcctcgacga gctcggtcac
     1981 gccgagcgtc gcctccgccg cgaggcggcc gagaccgcga aagtggccgg accgggagtg
     2041 cgggctcgtc gtcatcgttc gatcggggga cgctccgcgg agagtacccc gccgccccgg
     2101 tcagcccatg gcggcagtcg ataacgggcg cggcggcagg aacgcgcacg cggcgaggag
     2161 gaacgcgagg ccgtcgagcg gctgccggta ccgcgcctcg aaaccgacga ggtaatagac
     2221 gagagggaag aggaggagcg gagcgaggag cgccgcccgc cggtcggccg ggagtgacgg
     2281 gaacgcccgc cacgcgccga cagccgagag gaccgtgagg aggccggtcc cgagcatcgc
     2341 cacggggtcg tcgaccggcc cgagccagaa ctggaggacg cggagccccg tgagccggag
     2401 gaagtcgccg gggtgcgaga cgatccacgc ccgcgcctcg cggcccgccg cccgcatgta
     2461 ggcgagctcg ccgagctccg ccaccttccg ggcctcccct tcgtccgtcc gcgggtgccg
     2521 ctccgttccg ccccgggccg agaggtcgag gttcgcgccc gccccctcgt ggtttcccat
     2581 ccggagctcc agtccgaagt tgctccggag gaagaagagg ccgccgaggg cgcgttcgtt
     2641 gcgcagagtc caaggcacgc aggcgaggag catcccggcc gctacgagcg cggaacgaag
     2701 gagaccgggg cggcgggccg ctccgagcgc ctcccagacg aggaggccga gggccacggg
     2761 gaggagcgac ggggagacgt ggagcgccgc cccgaccgcg gcgccgagga ggagcgaccc
     2821 ggtggccgac gggcgcggcc cctcccatcg cgcgaggaac gcaacgagca tcagcccgat
     2881 cgcgatcgcc gaaggcgcct ccacgtaccc gggccagcga gggaggagcg cgccggcgag
     2941 ccccgcgagg agccccccgc ccgcggagag gccgagccgc gccgccagcc acgggagcat
     3001 cccccacatc gcgccgtgag acgcgatgag gaggaaccac cagacgtacc ccgccgcgag
     3061 gttcgggccg aggaggtggt agatccccgc gaggagcgcc gggtgaaacg gcggcatgtg
     3121 cgcggtcggc ccggttggca cgccgtaggg gtcggcgaac gcccctctct cgtagaggga
     3181 catcgccacg gccgtcacct cgaaccgggt gtgcggcatc acgtgccgga cgtcgagctt
     3241 cgtcaggaag aagccctgga acccgacgtg caggaggaag acgaggagga agccgcgccg
     3301 aggcgacgag aggagcccgg cggggtgcct gccttcgcct ccgtcgctct cgagcggcat
     3361 ccgggatcct ccgcctccat tctgctctcg gcaggtcaca ccgtccctcc gctatcctgc
     3421 cgccgcggcc cgcgcgagcc gggcccgccc gggggactct ctcctgaccg acgccttcct
     3481 ccgcctccgc ggcgcgcgcg agcacaacct cgccgggttc gacctcgaga tcccgcgggg
     3541 gaagctgacc gtcgtcaccg gcgtctcggg gtcgggcaaa tcgtcgctcg cgttcgacac
     3601 gctcctccgc gaggggcagc gccgcttcct cgagctgatg ccttcgtacg cgcggcagtt
     3661 cgcgggcggc ttccgccgcc ccgacctcct cggcgccgag gggctcggcc cggcggtcgc
     3721 cgtcgcgcag gacgtctcct cctcgaaccc gcgctccacg gtcgggacgc tcacggaggt
     3781 ccacgacctc ctccgcctcc tcttcgcgcg cctcggcgac gccccggccg gcctgaagcc
     3841 ggctcgcggc ctcttctcct ggaacggctc cggggcctgc cccgcctgtc gcggcctcgg
     3901 cctcgaggac cgcctcgacc cggcgctcct cgtcgcggac gggacgaaga cgatccgcga
     3961 gggggccctc cgcgtctcga cgccgaacgg ctacctcatg tactcgcagg tgacgctcgc
     4021 cgttctcgac gacgtcctcc gcgcgcacgg cggctccgtc gacgtgccgt ggcgcgacct
     4081 gacggacgag gtgagggagg tcgtcctccg cggctccgac cgcgtcctcg tcccgttcgg
     4141 caagcacccg ctcgagaacc ggctgaagtg gaaggggatc accgcgcggc cgcggcagga
     4201 ggggcactac cggggcctcc tcccggtcat ggaggagatc ctccgcggga agcgtaacga
     4261 ctcgatcctc cgcttcgtcc gctcgacgcc gtgcgcggcc tgcggcggga cgcgcctctc
     4321 gaaggaggcg ctctccgtca cctggcgcgg aaggacgatc gcggggcttt cgggccggac
     4381 cgcgcgcgag ctgcgcgccg tcttcgagga ggcgggtgac ggagaggttc tcgggccgat
     4441 ccgcgacgag gtcgtcgcgc gctgcgcgct cctggaagac ctcggcctcg gctatctctc
     4501 cttcgaccgc gccgcgccca cgctctctcc cggcgaggcg cgccgtctcc ggctcctctc
     4561 gctcgcgctc ggcgagctgc gcggcctcgt cgtcgtcctc gacgagccga gcgccggcct
     4621 ccacccgcac gacaccgccc gcctccttgg cgtcctgcgc cggctgaccg cgcgcgggca
     4681 gacggtcgtc gtcgtcgacc acgagccgct cctcgcccgg aacgcggact tcctcgtcga
     4741 cctcgggccg ggtccggcga aggagggcgg ccgtctcctc ttcgccggcg cgccggcgga
     4801 cctcctcggc cccgacggcc ccgatacgcc gacgcgccgc gcgctgcacg gcgagggtgc
     4861 ggcccttccc gtggcggcgc gggaggcgga cgagggcgag gacgtcgtcc tcgagggcct
     4921 cgcccggcac aacgccgccg gggcgacgct ccgcatccgc ccccgcgccc tcaacgtcgt
     4981 cacgggcgtc gcgggcgcgg ggaagacgtc gctccttcac gcggccgtcg cccggctccg
     5041 cgagagcggg tcgttcgcgc gcatcgtcgc cgtcgacgcc gagccgatcg gccggacgcc
     5101 ccgctcgaac gccgcgacct acacgggcgc tttcgacgtc atccgggacc tcttcgcggc
     5161 aacgcccgag gcgaaggcgc ggggcttcgg cagggggcac ttcacgttca acaccgcggg
     5221 cgggcgctgc gagtcgtgcg agggagcggg cgtccacgag gtcgggatgc gctggctcgg
     5281 ttccgtcgag ctcgtctgcg gcgcgtgcgg cgggcggcga ttccacgcgg acgtcctcgc
     5341 ggtgacgctc gacggcctct ccgtggccga cgtcctcgac ctgaccgtcg ccgaggccat
     5401 ggagcgcttc cgcaaggaga agaagctcgc ccgggtcctc tctgccctcg tcgacctcgg
     5461 cctcggctac ctcccgctcg gccaacccgc gacgacgttg tcggggggcg aggcgcagcg
     5521 ggtgaagctc gcgacggagc tggcgaaggc gacgggcccg gccctcttcg tcctcgacga
     5581 accgacgacc ggcctccacc ctgccgacgt cgcggtgctc gccgcggcct tccggcacct
     5641 ctgttcggcc aggcacacgc tcctcgtcgc ggaccacgac ctcgacctcc tccgcgccgc
     5701 cgaccacgtc accgaaatcg gcccggggag cggaccggag ggcgggcgtg tcgtcacgga
     5761 gggaacgagc gccgacctcg cggcgtgccc ggaatcgccg acgggcgccg cgctccgcgg
     5821 cgagatcggc gccgcgttgc cgtccgaagc gcaggccggg tcgcgcgggg acgagccgct
     5881 cgcgctctcg ggcgtgacga cgcacaacct ccggggcgtc gcggcgcgct ttccctgccc
     5941 gggtctcacg gtcgtcacag ggccgtccgg ctcggggaag tcgtcgctcg tcttcgacac
     6001 cctcctcgcc gaagcgcagg cgcgcttctc cgacctcctc tccccctggg cgcggcgctt
     6061 cctcccgatg aacggcggcg ccgagctgga gtcggcctcg gggcttcgcg ccgcggtcgc
     6121 cgttccggcc gatgccgccc gacgcaaccc gcgctcgacc gtcggaacga cgacggggat
     6181 cgacgagctc ctccgcctcc tcttcgcgcg cggcggcgcg cgcccctgcc ccgtctgcgg
     6241 ccgtgaggcc gcgggggcaa cctgcgcctg cggcgagcgg ctcgagccgc tcctcgcctc
     6301 cgacttctcg ccgcattccg agcggggcgc ctgcccctcc tgccgcggcc tcggcttcgc
     6361 cccggcgtgc gacccggcgc gcctcgtgag ccggccggac ctcccgctcg acggcggcgc
     6421 gctcgacggc acccgttacg gcgcctacct cggagaggcc gacggccagc acgtcgcgac
     6481 gctgcgcgcc gcggccgccg cgctcgggct cgacgtctcg gggccgtgga gcgccctctc
     6541 gcccgaggcg cgcgccctcg cgatggacgg cgccggcgag cggacgttcg aggtcgactg
     6601 gagctggaag cgcgggaagc ggagcggcgt ccaccgcctc gcggcaaagt gggccggcat
     6661 cgcgcggctc gtcgaagccg agtacgagag ggtccacgcc gacgaacggg gcgaggagct
     6721 cgagccgctc ctcgtcgacc gcccctgcac gacctgcggc ggcgagcggc tcgcgccgca
     6781 ctcccgcgcc gtgacgttcg ccggaaagcg cgcgcccgag accacggatt ccgagtccga
     6841 ctccctcgtc gcctggctcg acgggatcgc ggcccggcac gccggcgttg cgccctctct
     6901 cctcgccgcg acggctccgg tccgcgaggc gctcgcgccc cgcctgcgcg ccctctgcga
     6961 cgctggcctc ggctacctct cccccgcgcg cgagatggcc tccctctcgg gcggcgaggc
     7021 gcagcgcgtc cggctcgccg ccgcgctcgg cggcggcctc gtcggcgtga cgtacgtcct
     7081 cgacgagccg acgcaggggc tccacccgcg cgacacggag cgcctcgtcg gcgtgctccg
     7141 ttccctcgcc gacgcgggga acgccgtcgt cgtcgtggag cacgacgccg cgctcgtcgc
     7201 cgccgcggac cacgtcatcg agctcgggcc tctggccggc cccgagggcg gcctcgtcgt
     7261 ggcggatggg ccgcccgccg agctccgcgc cgacccttcg acgtacacgg cgcgcctcct
     7321 cgcccgcacc gacgcttcgc cttcccgcgc tccccgcgca ctcttccccg gcgtcaccgt
     7381 ccgcggcgcc caccgccaca acctcaagga cgtcgacgtc gactttcccg ccggcgggct
     7441 cgtcgtcgtc gccggcgtct ccgggagcgg caagtcgacg ctcgtcctcg aggtgctcgc
     7501 cccgtccctt cgcgccgcgc tccggggcga cgaccgcgtc ggctgccgcg ccctcgagcg
     7561 gcacgccgag atcgaagacg tcctggcctc ggaccaggag ggcctcgccg tcggcggcgg
     7621 cagcaccgtc gcgacgctga cgggagtggg agaggagctg cgccgtctcc tcgccgccac
     7681 gcccgaggcg aagagacgga agctcgccgc cacggctttc tcgacgaacg tcccgggcgg
     7741 gcgttgcgag tcctgcgccg gccgcggcgt cgtccgtgtg ccgatggacc tcctccccga
     7801 cgtgaccgtc ggctgcgaga cgtgcgccgg gcgccgcttc tcggacgacg tcctcgcctg
     7861 ccgtctcgac ggtcgttcga tcaccgacct cctcgacgcc ccggtctccg acgccacggc
     7921 gtggctcgcg gaccgcccgg cgctcgccgc gcccctcgcc gcgctccgcg acctcggcct
     7981 cggctacctc cgcgtgggcc aggaggggac gacgctctcc tcgggcgagc gacagcgcct
     8041 ccgcctcgcg cgcctcctcg tcgcgccacg tcccgaccgc gtcgccgtcc tcctcgacga
     8101 gccaacgcga ggtctcgccc tcgacgacgt cgatcgcctg ctctccgccc tcggccgcct
     8161 cgccgccgaa ggccacctcg tcgtcgtcgt cgagcaccac cccgccgtcc tcgccgcggc
     8221 cgaccgggtc gtcgagctcg ggccggaggg gggcgagcgc ggcggaagga tcgtccgggt
     8281 gagagaatga cggcaggcga ggcccgcgca ccactgtcat gaccgatcgc gaacagtcga
     8341 ccggaagcgg caccctggag gagagcgaga gcgtcctgcg cgaggagcgg gccctctaca
     8401 gggacctcgt cgaagcccag cctcgaggca tctaccggat ccgctcggtt tgcccgggag
     8461 ccgagctccc ggaggggagc ctcgagtccg gccgggcgcg gtacgtcgtg gagttcgtct
     8521 cgagccgctt cgtccagctc ctcggcatgg acccggagga gttccacgcc cgccacggcg
     8581 ccatcgacga cctgatccat ccggacgacc gaaggtcgtt cacagaggcg aacgaacgag
     8641 cgctcaccgg gcagctcccg ctccactggg agggccgcct cacggttcgc ggggcggcgg
     8701 cggcccgctg gttccggttc gagtccgtcc cgagggcgct cgaggcgggc ggcgccgtct
     8761 ggaccgggac gctcgaggac gtcacggagc agaggcgggc gattgcgagc ctgcgggagt
     8821 cggaggcgct cctccggagc ttcttcgagg tccccctctt cggcgcctgc atcacttccc
     8881 cgacgaagga ctggctcgag gtcaacgaca gcctctgccg catgctcgga tacacccggg
     8941 aggagctcgt gaagctcacc tggtccgagc tcacccatcc cgacgacctc gcggcggacg
     9001 tcgagcagtt cgaccgcgtg atccgaggag agatcgacgg ctactcgctc gagaagcgat
     9061 accttcggaa ggacggaagc gccgtcccgg tcgacctcgc cgtgagctgc gcgcggttcc
     9121 cggacggggc cgtcgaccac ctgatcgcga tcatctccga catcaccgag aggaagcggg
     9181 ccgaggagca gctccgctgt catcgcgagc ggctcgagga gctcgtcgcc gaaaggaccg
     9241 tccagctcga gaagagccgg gagctcctcg acgccacctc gcgcctcgcg cggatcggcg
     9301 gctgggagta cgacgtccgg accgagaggc tcacctggac cgagatggtc cgtgagatcc
     9361 acgaggtcgc tcccgggtac gacctcacgg tcgctgccgg gctcgacttt tacgccccgg
     9421 agtcgcgacc ggtcatcgag ccggccttcc gccgcgcggt ggcgtcggga gagcccttcg
     9481 acctcgagct ccagctcgtc acggcgacgg ggaaacgcct ctgggtccgc gcgatcggcc
     9541 accccacggt cgaggacggc gaggtcgtcc gggtcatcgg cgtcctccag gacatcgacg
     9601 cccggaggag gaccgaggag gacctggcgc gctaccggac ccggctcgag gagctcgtcg
     9661 ccgagaggac ggccgaggtg gagaaggcca acgcccgcct cgtcgaggcg caggcggtcg
     9721 cccacctcgc cagctgggag tgggatgcgc gcaacgacga gatccgtggg tcggaggagc
     9781 tctaccggct cttcggcgtc cctccggaag ggctcgcgcg cgtcgagcag ctcctggacc
     9841 tcgtccaccc cgacgaccgg gagagcgtgc ggatcgcgat cgaggaggcc cggactcacg
     9901 gcgacggcac tctcttcgag cagtcctacc gcgtcctcct cccggacggt cgactccgtt
     9961 tcgtcaatga ccgcggcgtc gtctccgtcg acgcggacgg tcagctgacc gggatgttcg
    10021 gcacctgcca cgacatcacg agcgtccgcg aggccgagga ggcgctccgg gagagcgagg
    10081 agcgcctccg gcgcatcgtc tccgaggcgc cgttcccgat cgggctctac gccgaggacg
    10141 gcgagatcct cctcgtgaac aacgcctggc ggcggatcac cggatacgct cccgaggagc
    10201 tccggacgat cggcgagtgg gcggcacgcg cctatggcga ccggagggag atcgcccgag
    10261 ccgacatcga gtcccgcttc tcgctctccg acgcgatcca cgagggcgag tacgccattc
    10321 ggacgaagag cggcgagagg cggtactggg acttcagctc gagccccgtc ggacgcctcc
    10381 ccgacggccg gcggctcgtg acgacgatgg cgatggacgt caccgagcgc cgtcgggccg
    10441 aggaggaggt gtatgcctac cgggagcacc tcgaggagct cgtccgggaa aggaccgccg
    10501 agctcgaggc cgcgaaccgg gagatggcga ccttctccta cagcgtctcc cacgacctgc
    10561 gcgcgccgct ccgggcgctc gacggcttca gcgcgatcct cgcgcaggag tacccggacc
    10621 gcctcgacga gcgggggcgc ggctacctcg accggatccg ggccgcggcc cggaccatgg
    10681 cgctcctcgt cgacggtctc ctcagcctcg cccagctcaa ccggcaggag ctgaccgtcg
    10741 ccccggtcga cctctccgcc ctcgcccgaa aaatcggggc ggacctgacg tcgctggagc
    10801 cggggcgcgt cgtccggctg atcgtgccgg acggtctgac gaccgacggg gacccccgcc
    10861 tcctgcacgt cctcctcgag aacctgatcg ggaacgccct gaagttcacc tccttgcgtc
    10921 cggaggcgac gatcgagctg gggcgcgcgg agcgcgacgg cgagacggtc ttcttcgtga
    10981 aggacaacgg cgtcggcttc gacatgacgt acgcctcgaa gctcttcctg cccttccagc
    11041 gcctccacag cgtgaacgag ttcccgggaa cgggaatcgg cctcgcgaca gttcaccgga
    11101 tcgtcgcgcg ccacggcgga cggatctggc ccgaatcgag cctcgacgcc ggcgcgacgt
    11161 tctacttcac tctcggcgcc gcggaaggct gacgccgccg ggggcctccc ggtcgaggac
    11221 ctcccggacc tttcgcagca ggtccagcgg ggtgacgggc ttcatcagga gctcgaagcc
    11281 ctcgagggcc ggctcccggc tcccgacgag ctccggagag tagccgctcg agaagagcac
    11341 cttcacgtca gggcgcagcc tgcggatctc ggtggcggct tcccggcctc ccttcctcgg
    11401 catgatcatg tccaggagga cgaggtgcac gtcgccgcgg cg
//
LOCUS       DAOTEZ010000092        11418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3984_length_11418_cov_5.734049, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000092 DAOTEZ010000000
VERSION     DAOTEZ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11418
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3984_length_11418_cov_5.734049"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="P5164_06750"
     CDS             complement(<1..370)
                     /locus_tag="P5164_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRY43631.1"
                     /translation="MRRIAVASALAFGLLLPVLSARPARAASPAPVSPAELGAHIRFL
                     ADDLLEGRAPGTRGDGLAQAYIESVFRANGLAPAFGSSYRQPVPIRIVVPDPKTTLTW
                     TGAAATAPAGRFGDDFVLALP"
     gene            complement(411..2273)
                     /locus_tag="P5164_06755"
     CDS             complement(411..2273)
                     /locus_tag="P5164_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRY43632.1"
                     /translation="MAAPPIASSPAPRSSSASASPATAIEARHVAHVMDEKLGERGDG
                     TALRYLDAGQWRDVSWRELRGSVRDLSSALVEAGVAEGDRVAILAANRPEWTMADLAV
                     LRVRGVSVPIHATSSASQVAFILKDSGARFVFVDGGEPLAKVVAAIEAGVPIERVVVF
                     GGGALPEGAVFAGLEETLTRGRSAGREHEITARLSRATPDDLLTLLYTSGTTGEPKGV
                     MLPHSAFTVTCAYHDLRLPPIGADDVSLCFLPLSHIFERAWTFYILYRGAVTAYCEDP
                     KKVSEVLPQVRPTLMCAVPRLYEKAYTKIQERVRSSSAAKRALFRWAIGTGHAVSLRR
                     RAGETVPQLLALRHALADRLVLAKIRAAFGGRPRLFPCAGAPLAPEIEEFFHSVGVFV
                     CQGYGLTETTATVTCHDPSRFHVGTVGSPLPGVEVRISEEGEVLVKGRTVMKGYWNRP
                     EETAAVFRDGWLRTGDAGEICEDGTLKITDRIKDLIKTAGGRYVAPQHVETTIGADPL
                     VEQVAVIGEGRSYVTALVVPAFEALEKWARAAGISFSGRDDLVRNERVLDLLKRTIEE
                     RTKDLAPWEKVRKFSLLTKEFSVEGGSMTPTQKVRRRAAAELYRDRIEEMYTKD"
     gene            complement(2325..3794)
                     /locus_tag="P5164_06760"
     CDS             complement(2325..3794)
                     /locus_tag="P5164_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY43633.1"
                     /translation="MSRATAAAVAACLVAVAACERPAPPVRIGIHSAPVDLDPHLQNE
                     SLTSAVLANVYEALTELDEETRVRPKVASGWTNPDERTWVFTLRQGARFHDGRAVTSA
                     DVVFSLERARRHPGSPLASYLVEVESVRALDEGRVEIRTRRPFAALLAKLTPVYIVPA
                     GSPARITAPIGTGPYRLVRADGRRLDLVPAATAGDRPTAIGPLSFLVERDPARRLSAL
                     LAGEVDVAADLEEDAVATLASSDCCRAEFLPGSTIEYLHLSGREAPFRDPRVREAVHL
                     SLDRPRYLAEARHGLGQPAGQLAVPGLFGYEPGLRAPERDLARARALLEAAGYAGGFD
                     VVLEHRPGRRADVLARQLGEAGIRVVTRESTWTDLYRRLRRGEVGFYFGGVISPTGEA
                     SDVLDGYVHSREELRGYGASNHSRYSNRTADALIEAAGSSFSLVRRRELLQEAMRVVM
                     ADLHFVPVAGLYQVYGVRRDVEFRPRVDLKLLGDRIRRR"
     gene            complement(3791..5260)
                     /locus_tag="P5164_06765"
     CDS             complement(3791..5260)
                     /locus_tag="P5164_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY43634.1"
                     /translation="MRRAAALLALALGAAPAACRDGVKPVRIAVHSAPQSFDPHLQNE
                     ILTSAVLANLYDGLTEFDEESRVVPALASEWRNPDERTWVFRLRSGVVFHDGRPLTAA
                     DVLFSLERARHHSGSGLASYLVEIDSVRVPDPATIEIRTRRPFAAFLAKLTAIAIVPA
                     NAPPEIVDPVGTGSYRLARATEETVELVPAVNDWRRGRPLPVTFVVDGDPRGRLQRLL
                     SGDVDIAADLAENDVARIGESECCRAIAQPGSTVEYLRLSPTAPPFRDRRVRQAIDLA
                     LDRSAYLADAHGGIGQPAGQLCVPGVFGFAPDLKATVRDLPRARRILEEAGFGGGLDL
                     VLEHRPGRRGDVLARQLAEAGIRATPRVTPWTELHPRIRHGEVDFYFGGVVAQTAEAS
                     DVLDGFVHTRDESRGYGSTNHSRYSNPTADALIESASAALDLRRRRDLLQRAMRVVMD
                     DLYLLPIAGLYDVYGLRREIAFEPRFDRRLPGRGIDRTR"
     gene            5445..6407
                     /locus_tag="P5164_06770"
     CDS             5445..6407
                     /locus_tag="P5164_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HRY43635.1"
                     /translation="MTKPPSRPSPAELLEKMQLIRSFEERSAASFAEGRSPGTCTCVG
                     QEASAVGVVAALREDDLILTNHRSAGHLLARGADPGRLMAEVMGRATGYCGGKSGTLH
                     VSAKELGVILTSTIVGGELSLAPGVALSVSMKGGDEIVTCFFGDGAACEGIFHEALNL
                     AAVWKLPVLFVCENNEWQAFVHRRETMLTENIASRAAGYGIEGRTVDGNDAEAVFAAA
                     SEAIARIRETKMPFLLETKTYRLRGHFEPDDQGYVDKAELASWKARDPIAALRASLLE
                     SGALTAGGAAAIERRVAERLDAAFTFAADSPWPSLDELTTHVYA"
     gene            6421..7401
                     /locus_tag="P5164_06775"
     CDS             6421..7401
                     /locus_tag="P5164_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRY43636.1"
                     /translation="MKTMTPYDAIGEALAEEMRRDATVLAFGEGIATKRHDLVAEFGA
                     ARIRNTPLAEGIIAGTAAGAAATGLRPVVDLLFAPFLTFAMDELVNSAGKLRFLSGGQ
                     FSFPMVALAMTGAGWGVGAQHNHNVEAWFAHSPGLKVVMPSTAADFKGLLKSSIRDDN
                     PVAFFLDVALLHAPGEVPEGEHVVPLGKAAIRRTGSDVTLVSYAKTVLGCLAAAETLA
                     AEGISAEVIDLRTIKPWDEEAVLASVKKTGRLVVVHEASRTAGFGAEIAATVAEKAFE
                     ALRAPIVRLAGPDAPAASSWVLEQAFVPQADAIAGAARTAVARAMVLATA"
     gene            7554..9278
                     /locus_tag="P5164_06780"
     CDS             7554..9278
                     /locus_tag="P5164_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43637.1"
                     /translation="MKVARPVLSVPLLVALVSVAAAPASTPEDVAPPAAWRPFELGSY
                     TRPVSTRVAEAQKAFDQGLVWAFAFNHDEAERAFAESARRDPSLAMAWWGVALVNGPH
                     INNPVMSEAQSRKAWDALTKARALAPGASEVEKALIEALGARYASPPPPDRAPLDAAY
                     AKAMLAVRSRFPADADVATLAAEALMDTRPWDQWTRDGKPQPGTAEVLAGLEEARRLA
                     PDHPGALHLTIHALEASPRPERATEAADRLRALVPDSSHLVHMPSHIDVRVGRWKEAA
                     EANERAMAADRRYQGRTGQIGFYRIYMAHNAHFLAYTAMMEGRREVALATATGVVAGL
                     DPAFVEENAAFADAFLTVVGEARKRFGLWKEILAAKAPPEGLPVSAAHAHFLRGVAHA
                     ALGHVAEAKRERAAFAAALPKVPKEFYWGSNLAADVLAVGLPYLDGEIAYRLGDHAGA
                     VEKLREAVRLEDALKYDEPPAWTVPSRHALGAILLDARRPAEAEAVYREDLRTYPGNG
                     WSLRGLATALEMQKKSAEAAAVEERFTAAWARADTSLETSCFCVRKSRSDAPAGTSAL
                     AQARTTRR"
     gene            9404..>11418
                     /gene="gltA"
                     /locus_tag="P5164_06785"
     CDS             9404..>11418
                     /gene="gltA"
                     /locus_tag="P5164_06785"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HRY43638.1"
                     /translation="MYQIVKREQFSDVTFLWDVHAPDVAKAARPGHFVMLRLDEGGER
                     IPLTVADYDPEWGTVTIVIQALGKTTREMMRAYKEGDTFADFVGPLGLESHVGKVGHV
                     VLVGGGLGVAPVFPQLRAFKEAGNRTTGIIGFRNRDLVFWEDRFKRYCDDLIVCTDDG
                     SYGKPGFVTAALQEVLEKEKPDLVVAIGPLPMMRACSETSRPFGVKTMVSLNAIMVDG
                     TGMCGSCRVTVGGETKFACVDGPDFDGHLVDFPELQLRQERFKPQETQAKDDFSKVCS
                     IEEQLFEMNKRNYKKFKDLDEHAVKMPERNALERSRNFEEVNLGYTMADALQEADRCI
                     QCARPTCVAGCPVSIDIPRFIRHLLVRDLEGALSVIQESNLFPSICGRVCPQESQCEI
                     QCILEKKMEPVAIGRLERFVGDFAPLPDVKPPENAGTLGKVAVVGSGPAGLACAGDLA
                     RKGASVTVFEALHVVGGVLKYGIPSFRLPRTTIDREVKALEKLGVKFETNKVIGKTFT
                     IDQLKGEMGYDAVFIGTGAGFPMFLGIPGEHAGQVYSANEFLTRINLMHGNKFPFEDT
                     PIAMGKKVAVFGAGNTAMDCLRVARRLGAEEVHCIYRRTEAEAPARVEEIRHAKEEGI
                     HFNWLMSPTEIQVDEKGDVKAVVVEKMQLGEPDKSGRRRPVPTGEHET"
BASE COUNT         1678 a         4172 c         4056 g         1512 t
ORIGIN      
        1 gcgggagcgc gaggacgaag tcgtcgccga accggccggc cggagccgtc gccgccgcgc
       61 ccgtccacgt cagcgtcgtc ttcggatcgg gcacgacgat gcggatcggg accggctggc
      121 ggtagctcga gccgaaggcg ggcgcgaggc cgttcgcccg gaagaccgac tcgatgtacg
      181 cctgcgcgag gccgtcgccg cgcgtcccgg gggcgcgccc ctcgaggagg tcgtcggcga
      241 ggaaccggat gtgggcgccg agctcggccg gcgacacggg cgcgggagag gccgcgcggg
      301 cggggcgagc ggaaaggacg gggaggagga ggccgaaggc gagagcggag gcgacggcga
      361 ttcgacgcac gggatctcga gtatagaggc ggcgcgctcc ccgcccgacg tcagtccttc
      421 gtgtacatct cctcgatccg gtccctgtag agctcggcgg cggcccggcg gcgcaccttc
      481 tgcgtcggcg tcatcgagcc cccttccacg gagaattcct tcgtcaggag cgagaacttc
      541 cgcaccttct cccacggggc gaggtccttc gtccgttcct cgatcgtccg tttgaggaga
      601 tcgaggaccc gctcgttccg cacgaggtcg tctcgccccg agaaggagat cccggcggcc
      661 cgggcccact tctcgagcgc ctcgaacgcc ggcacgacga gggcggtgac gtagctgcgc
      721 ccttccccga tcacggccac ctgctcgacg agcgggtcgg cgccgatcgt cgtctcgacg
      781 tgctgcgggg cgacgtacct tccgcccgcc gtcttgatca ggtccttgat ccggtccgtg
      841 atcttcagag tcccgtcctc gcagatctcg ccggcgtcgc ccgtccgaag ccagccgtcc
      901 cggaagacgg ccgccgtctc ctccggccgg ttccagtagc ccttcatgac ggtccgcccc
      961 ttcacgagga cttctccctc ctccgagatg cgcacctcga cccccgggag cggcgagccg
     1021 acggtcccga cgtggaaccg cgacgggtcg tggcaggtga cggtggccgt cgtctccgtg
     1081 aggccgtagc cctggcagac gaagacgccg acggagtgga agaactcctc gatctcgggg
     1141 gcgagcggcg ccccggcgca cgggaagagg cgcgggcgac cgccgaacgc ggcccggatc
     1201 ttcgcgagga cgagccggtc cgcgagagcg tgacggaggg cgaggagctg cggcacggtc
     1261 tcgccggccc tcctccggag gctcaccgcg tgtccggtcc cgatcgccca gcggaagagc
     1321 gcccgcttcg ccgccgacga gctccggacc ctctcctgga tcttcgtgta ggccttttcg
     1381 tagaggcggg ggacggcgca catgagcgtc ggcctcacct gcgggaggac ttcggagacc
     1441 ttcttcgggt cctcgcagta ggccgtcacg gccccgcggt agaggatgta gaacgtccac
     1501 gcccgctcga agatgtggct gagcggaagg aaacagaggg agacgtcgtc agcaccgatc
     1561 ggcgggagcc ggaggtcgtg gtaggcgcac gtcacggtga acgccgaatg cgggagcatg
     1621 acccccttcg gctcgccggt cgtcccggag gtgtagagga gcgtcaggag gtcgtccggc
     1681 gtcgcgcggg agagccgcgc cgtgatctcg tgctcgcgcc ccgcggagcg tccccgcgtg
     1741 agcgtctcct cgaggcccgc gaagaccgcc ccctcgggga gagctcctcc gccgaagacg
     1801 acgacccgct cgatcggcac gccggcctcg atcgcggcga cgaccttcgc cagcggctcg
     1861 cccccgtcca cgaagacgaa gcgcgctccc gagtccttca ggatgaacgc cacctggctc
     1921 gcgctgctcg tggcgtggat cgggaccgag acgccccgga cgcggaggac cgcgaggtcg
     1981 gccatcgtcc actcggggcg gttcgcggcc aggatcgcga cgcgatcgcc ctcggcgacc
     2041 cccgcctcga cgagggcgga cgagaggtcc cggaccgatc ccctgagctc gcgccacgag
     2101 acgtcccgcc actgcccggc gtcgaggtaa cggagcgctg tcccgtctcc gcgctcgccg
     2161 agcttctcgt ccatcacgtg ggcgacgtga cgggcctcga tcgcggtggc cggcgaggcc
     2221 gaagcggacg aggagcgggg agccggagag gaggcgatcg gaggggctgc catcccgtcg
     2281 atcgtaccgc gcgggccggc gggcggagga tgagaagccc cgacctacct gcgtcggatg
     2341 cgatctccga gcagcttcag gtcgacgcgc ggccggaact cgacgtcgcg cctcacgccg
     2401 tagacctggt agagacccgc cacggggacg aagtggaggt cggccatgac gacccgcatc
     2461 gcctcctgca ggagctcccg ccgccggacg agcgagaacg acgatcccgc ggcctcgatc
     2521 agcgcgtcgg ccgtccggtt cgagtatcgc gaatggttcg aagccccgta gccgcgaagc
     2581 tcctctcgcg agtggacgta gccgtcgagg acgtcgctcg cctcgcccgt cggcgagatg
     2641 acgcccccga agtagaagcc gacctctcct ctccggagcc tccggtagag gtccgtccag
     2701 gtcgattccc gcgtcaccac ccggatcccg gcctcgccga gctgccgcgc gaggacgtcg
     2761 gcccgccggc cgggccggtg ctcgaggacg acgtcgaagc cgcccgcgta gccggcggcc
     2821 tcgaggagcg cgcgcgcccg ggcgaggtcc ctctccgggg cgcggaggcc cggctcgtag
     2881 ccgaagagcc ccggaacggc gagctggccc gccggctggc ccagcccgtg gcgggcctcg
     2941 gcgaggtagc gcggacgatc gagggagaga tggaccgcct cgcggacacg ggggtcccga
     3001 aacggcgcct cgcggcccga gaggtggaga tactcgatcg tcgagccggg aaggaactcc
     3061 gcccggcagc agtccgacga ggcgagggtc gcgacggcgt cctcctcgag gtcggcggcc
     3121 acgtcgacct ccccggcgag aagcgccgag agcctccggg ccgggtctct ctccacgagg
     3181 aaggacagcg ggccgatcgc cgtcggacga tccccggccg tcgcggcggg aacgaggtcc
     3241 agccggcgcc cgtcggcgcg gacgaggcga tacggacccg ttccgatcgg cgcggtgatc
     3301 cgcgcgggcg atccggccgg cacgatgtag acgggggtca gcttcgcgag gagcgccgcg
     3361 aacggccgcc gcgtccggat ctccacccgc ccttcgtcca aggcccggac ggactcgacc
     3421 tcgacgaggt agctcgcgag cggggacccc ggatggcggc gcgcccgctc gaggctgaag
     3481 acgacgtccg cggacgtgac cgcgcgtccg tcgtggaagc gcgccccctg ccgcagcgtg
     3541 aagacccacg tcctctcgtc ggggttcgtc catcccgatg cgactttcgg ccggacgcgc
     3601 gtctcctcgt ccagctccgt gagggcctcg tacacgttcg cgaggaccgc cgaggtcagc
     3661 gactcgttct gcaggtgcgg gtcgaggtcc accggcgcgc tgtggatccc gatccgaacc
     3721 gggggcgcgg gccgctcgca cgccgcgacg gcgacgaggc acgcggccac ggccgcggcg
     3781 gtcgcacggc tcatcgcgtc cggtcgatcc cgcggccggg aagcctgcgg tcgaacctcg
     3841 gctcgaacgc gatctccctt cgcaggccgt agacgtcgta cagcccggcg atcggcagga
     3901 ggtagaggtc gtccatcacg acccgcatcg cccgctggag gaggtccctc ctccgccgaa
     3961 ggtcgagcgc cgcggaggcc gactcgatca gcgcgtcggc cgtggggttc gagtagcgcg
     4021 agtggttcgt cgagccgtag ccccgcgact cgtcgcgcgt gtgaacgaag ccgtccagga
     4081 cgtcgctcgc ctcggccgtc tgcgccacga cgccgccgaa gtagaagtcg acctccccgt
     4141 ggcggatcct cgggtggagc tcggtccagg gcgtgacgcg gggcgtcgcc cggatgccgg
     4201 cctcggcgag ctggcgcgcg aggacgtcgc ctcgccggcc cgggcgatgc tcgaggacga
     4261 ggtccaggcc gcccccgaac cccgcctcct cgagaatgcg ccgcgcgcgc gggaggtcgc
     4321 ggaccgtcgc cttcaggtcg ggcgcgaagc cgaagacgcc cggcacgcag agctggcccg
     4381 ccggctggcc gatgccaccg tgcgcatcgg cgagatacgc ggagcggtcg agcgcgaggt
     4441 cgatcgcctg ccggacgcgg cggtcgcgga acggcggcgc cgtcggcgac agccgcaggt
     4501 actcgacggt cgagcccggc tgcgcgatcg cccggcagca ctcggactcc ccgatccgcg
     4561 ccacgtcgtt ctcggcgagg tccgcggcga tgtcgacgtc cccggacagg agccgctgaa
     4621 gccggccgcg cggatcgccg tccacgacga acgtcacggg cagggggcgg ccccggcgcc
     4681 agtcgtttac cgccggaacg agctcgacgg tctcctccgt cgcccgcgcg agccggtacg
     4741 agccggttcc gaccgggtcg acgatctcgg gcggggcgtt cgccgggacg atggcgatcg
     4801 cggtgagctt cgcgaggaac gccgcgaacg gccgtctcgt ccggatctcg atcgtcgcgg
     4861 gatcggggac ccggaccgaa tcgatctcca ccaggtagct cgcgaggccg ctccccgagt
     4921 ggtggcgcgc ccgctcgagg ctgaagagca cgtccgccgc cgtcaggggc cgcccgtcgt
     4981 gaaacacgac gcccgaacgc agccggaaga cccaggtcct ctcgtccggg tttcgccact
     5041 cggaggcgag cgccggcacg acccgcgact cctcgtcgaa ctccgtcagc ccgtcgtaga
     5101 ggttcgcgag gacggccgac gtcaggatct cgttctgaag gtgcgggtcg aagctctgcg
     5161 gggcgctgtg gacggcgatg cggacgggct tcacgccatc ccggcacgcc gccggtgccg
     5221 ccccgagggc gagcgcgagg agggccgccg cgcgcctcac tcgtccagta tgccagccac
     5281 ccgccgtctc gccccccgat tcgcgcatag cgatttctta ggatcgctta gtcgattctt
     5341 aggtggcggc ccgccctccg ccgccgcatc ttccacggcg tcgggagcgc cccccgggaa
     5401 cgccccgccg agacaggcgc cccgcgccgt atacgaggtg aacgatgacg aagcccccgt
     5461 cccgtccgag ccctgccgag ctcctagaga agatgcagct catccgctcc ttcgaggaga
     5521 ggagcgccgc ctccttcgcc gaggggcgct ctcccggcac ctgcacctgc gtcgggcagg
     5581 aagcctccgc cgtcggcgtc gtcgccgcgc tgcgggagga cgacctgatc ctgacgaacc
     5641 accggagcgc cggccacctc ctcgcccgcg gcgccgaccc gggccgcctc atggccgagg
     5701 tcatgggccg cgcgacgggc tactgcggcg gcaagagcgg cacgctccac gtctccgcga
     5761 aagagctcgg cgtgatcctc acgtccacga tcgtcggcgg cgagctctcg ctcgcgcccg
     5821 gcgtcgcgct ctcggtctcg atgaaggggg gcgacgagat cgtcacctgc ttcttcggcg
     5881 acggggccgc ctgcgagggg atcttccacg aggcgctgaa cctcgccgcg gtctggaagc
     5941 tcccggtcct cttcgtctgc gagaacaacg agtggcaggc cttcgtccac cgccgcgaga
     6001 cgatgctgac cgagaacatc gcctcccgcg ccgcgggcta cggcatcgag ggccggaccg
     6061 tggacgggaa cgacgccgag gccgtcttcg ccgccgcgtc cgaggccatc gcccggatcc
     6121 gggagacgaa gatgccgttc ctcctcgaga cgaagaccta ccgcctccgc ggccacttcg
     6181 agccggacga ccagggctac gtcgacaagg ccgagctcgc gtcctggaag gcccgcgacc
     6241 cgatcgcggc cctccgcgcc agcctcctcg agagcggcgc gctcaccgcc gggggcgcgg
     6301 ccgccatcga gcggcgcgtc gcagagcgtc tcgacgccgc cttcacgttc gccgccgact
     6361 ccccctggcc ctcgctcgac gagctgacca cccacgtcta cgcctgagaa ggaagccgcc
     6421 atgaagacga tgacccccta cgacgccatc ggagaggccc tcgccgagga gatgcgacgc
     6481 gacgcgaccg tcctcgcgtt cggcgaaggg atcgccacga agcgccacga cctcgtcgcc
     6541 gagttcggcg ccgcccggat ccgcaacacg cccctcgccg aggggatcat cgccggcacc
     6601 gccgccggcg cggcggcgac gggcctccgc cccgtcgtcg acctcctctt cgccccgttc
     6661 ctcacgttcg ccatggacga gctcgtcaac agcgccggca agctccgctt cctgtccggc
     6721 gggcagttct ccttcccgat ggtcgccctc gcgatgacgg gcgccggctg gggcgtcggc
     6781 gcccagcaca accacaacgt cgaggcctgg ttcgcccact cgcccggcct gaaggtcgtc
     6841 atgccgtcca cggccgccga cttcaagggg ctcctgaagt cctcgatccg cgacgacaac
     6901 ccggtcgcct tcttcctcga cgtcgcgctc ctccacgctc ccggcgaggt gccggagggc
     6961 gagcacgtcg tcccgctcgg gaaggccgcg atccggcgaa ccggctccga cgtgacgctc
     7021 gtctcgtacg cgaagacggt cctcggctgc ctggccgccg ccgagacgct cgccgccgag
     7081 gggatctccg ccgaggtgat cgacctccgg acgatcaagc cctgggacga ggaggcggtt
     7141 ctcgcgtccg tgaagaagac gggccgcctc gtcgtcgtcc acgaggcgag ccggacagcc
     7201 ggcttcggcg cggagatcgc ggcgacggtc gccgagaagg cgttcgaagc gctgcgcgct
     7261 ccaatcgttc ggctggcggg acccgacgct cccgcggcct cgagctgggt cctcgagcag
     7321 gcgttcgtcc cgcaggccga cgcgatcgcc ggagccgcgc ggaccgccgt cgcgagggcg
     7381 atggtgctgg cgacggcctg agcacggcga gcggccgcca cgcgcgaggg tcggcggagg
     7441 gcggttccga gccctcgcgc gtggtctcgt cccgacccgg cgggggccga tttcggttga
     7501 cggcagcccg ggcaggctcc agattctgcg aagccgtcgc accggaggtc tccatgaagg
     7561 tcgcccgccc cgtcctctcc gttcccctgc tcgtcgcgct cgtgtcggtt gcggccgcgc
     7621 cggcttccac gcccgaggac gtcgccccgc cggccgcctg gcgccccttc gagctcggct
     7681 cgtacacgcg gcccgtctcg acgcgggtcg ccgaggccca gaaggcgttc gaccaggggc
     7741 tcgtctgggc cttcgcgttc aaccacgacg aggccgagcg ggccttcgcc gagtcggcgc
     7801 ggcgcgaccc ttcgctcgcg atggcgtggt ggggcgtcgc gctcgtcaac ggcccgcaca
     7861 tcaacaaccc cgtcatgagc gaggcgcagt cgaggaaggc gtgggacgcc ctgacgaagg
     7921 cccgggcgct cgcgcccggc gcgagcgagg tcgagaaggc cctgatcgag gcgctcggag
     7981 cgcgctacgc gtcgccgccg cctccggacc gggcgccgct cgacgccgcc tacgcgaagg
     8041 cgatgctcgc cgtccggagc cgtttccccg ccgacgcgga cgtcgcaacc ctcgccgcgg
     8101 aggccctcat ggacacccgt ccctgggacc agtggacgag ggacggcaag ccccagccgg
     8161 ggacggcgga ggtgctcgcc ggcctcgagg aagcgcgccg tctcgcgccc gaccaccccg
     8221 gagctctcca cctgacgatc cacgcgctcg aggcctcgcc ccggccggag cgcgccaccg
     8281 aggcggccga ccgactgcgg gccctcgttc ccgactcgag ccatctcgtc cacatgcctt
     8341 cccacatcga cgtgcgggtg ggccgctgga aggaggccgc ggaggcgaac gagcgggcga
     8401 tggccgccga ccggaggtac cagggccgca ccggccagat cggcttctac cggatctaca
     8461 tggcccacaa cgcccacttc ctcgcctaca ccgcgatgat ggagggtcgg cgcgaggtgg
     8521 cgctcgcgac ggcgacgggc gtcgtcgccg gcctcgaccc ggcgttcgtg gaggagaacg
     8581 cggccttcgc cgacgccttc ctgacggtcg tcggggaggc gcggaagagg ttcggactct
     8641 ggaaggagat cctcgccgcg aaggcgcccc ccgaaggtct cccggtctcg gcggcgcacg
     8701 cgcacttcct gaggggagtg gcgcacgccg cgctcggcca cgtcgcggag gcgaagaggg
     8761 agcgggccgc gttcgccgcg gctctcccga aggttccgaa ggagttctac tgggggtcga
     8821 acctcgcggc ggacgtcctc gcggtcgggc ttccctacct cgacggcgag atcgcctacc
     8881 ggctcggaga ccacgcggga gccgtcgaga agctccggga ggccgtccgc ctcgaggatg
     8941 cgctgaagta cgacgagccg ccggcctgga ccgtcccctc gcgccatgcg ctcggcgcga
     9001 tcctcctcga cgccaggcgg cccgcggagg ccgaggcggt ctaccgcgag gacctgcgga
     9061 cgtatcccgg gaacggctgg tccctccggg gcctcgcgac ggcgctggag atgcagaaga
     9121 agtccgccga ggcggcggcc gtcgaggaac ggttcaccgc cgcctgggcg cgcgccgaca
     9181 cgagcctcga gacctcctgc ttctgcgtgc ggaagagccg ctccgacgcc cccgccggga
     9241 cgtcggccct cgcgcaggcg cggacgacgc gtcggtaggc cgcgaagccc gcaccgggca
     9301 ggaccgtccg cagccgcggg agcgccgaag cgccgtttcg ggcatcatcc gcggtcgggt
     9361 tcggcggtcg cagcgtcccg cagggccccg gcaaggggga ttcgtgtacc agatcgtcaa
     9421 gcgcgagcag ttctccgacg tgaccttcct ctgggacgtc cacgctcccg acgtcgcgaa
     9481 ggcggcccgg ccgggccact tcgtcatgct ccggctggac gaggggggcg agcggatccc
     9541 gctcaccgtc gccgactacg accccgagtg ggggacggtc acgatcgtga tccaggcgct
     9601 cggcaagacg acgcgggaga tgatgcgggc ctacaaggaa ggcgacacgt tcgccgactt
     9661 cgtcgggccg ctcggcctcg agagccacgt cgggaaggtc ggccacgtcg tcctcgtcgg
     9721 cggcggcctc ggcgtcgctc ccgtcttccc ccagctgcgc gccttcaagg aggccgggaa
     9781 ccggacgacc gggatcatcg gcttccgcaa cagggacctc gtcttctggg aagaccgctt
     9841 caagaggtac tgcgacgacc tgatcgtctg caccgacgac ggctcctacg gcaagcccgg
     9901 tttcgtcacc gcggcgctcc aggaggtcct cgagaaggag aagcccgacc tcgtcgtcgc
     9961 gatcgggccg ctcccgatga tgcgcgcctg ctcggagacc tcgcgcccgt tcggcgtgaa
    10021 gaccatggtc tcgctgaacg cgatcatggt cgacggcacc ggcatgtgcg gctcgtgccg
    10081 cgtcacggtc ggcggcgaga cgaagttcgc ctgcgtcgac ggccccgact tcgacggcca
    10141 cctcgtcgac ttccccgagc tccagcttcg gcaggagcgc ttcaagccgc aggagacgca
    10201 ggcgaaggac gacttctcga aggtctgctc catcgaggag cagctcttcg agatgaacaa
    10261 gcggaactac aagaagttca aggacctcga cgagcacgcc gtgaagatgc cggagaggaa
    10321 cgccctcgag cgctcccgca acttcgagga ggtcaacctc ggctacacga tggccgacgc
    10381 gctccaggag gcggaccggt gcatccagtg cgcccgcccc acctgcgtcg cgggctgccc
    10441 cgtctcgatc gacatccccc gcttcatccg gcacctcctc gtccgcgacc tcgaaggcgc
    10501 cctctccgtc atccaggaga gcaacctctt cccgtcgatc tgcggccgcg tctgcccgca
    10561 ggaatcgcag tgcgagatcc agtgcatcct ggagaagaag atggagccgg tcgccatcgg
    10621 gcggctcgag cgcttcgtcg gcgacttcgc gcctctgccg gacgtgaagc cccccgagaa
    10681 cgccgggacg ctcgggaagg tggcggtcgt cggctccggg ccggccggcc tcgcctgcgc
    10741 gggcgacctc gcccgcaagg gggcctcggt cacggtcttc gaggcgctcc acgtcgtcgg
    10801 cggcgtcctg aagtacggca tcccgtcgtt ccgtctcccc cgcaccacga tcgaccgcga
    10861 ggtgaaggcg ctcgagaagc tcggcgtgaa gttcgagacg aacaaggtca tcggcaagac
    10921 cttcacgatc gaccagctca agggggagat gggctacgac gccgtcttca tcggcacggg
    10981 agccggcttc ccgatgttcc tcggcatccc gggcgagcac gccggccagg tctacagcgc
    11041 caacgagttc ctgacgcgca tcaacctgat gcacgggaac aagttcccct tcgaggacac
    11101 gccgatcgcc atgggcaaga aggtggccgt cttcggcgcg ggcaacaccg cgatggactg
    11161 cctccgcgtc gcgcggcgcc tcggggccga ggaggtccac tgcatctacc ggaggaccga
    11221 ggccgaggcc ccggcccgcg tcgaggagat ccgccacgcg aaggaggagg ggatccactt
    11281 caactggctg atgagcccga cggagatcca ggtcgacgag aagggggacg tgaaggccgt
    11341 cgtcgtcgag aagatgcagc tcggcgagcc cgacaagtcg ggccggcgcc gccccgtccc
    11401 gacgggcgag cacgagac
//
LOCUS       DAOTEZ010000093        11397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_3989_length_11397_cov_6.087727, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000093 DAOTEZ010000000
VERSION     DAOTEZ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11397
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3989_length_11397_cov_6.087727"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..931)
                     /locus_tag="P5164_06790"
     CDS             complement(245..931)
                     /locus_tag="P5164_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43639.1"
                     /translation="MRTARLALALFALSFAACKAREAAPPAPEPPAPVAPIEAAPPPT
                     SPAPPEAETAPTPPEIATPAAPPAAPTIVPAKPKPTTVPPKAAPTAPPAAPPTVVPPP
                     TVVPTTPPKPTAAPAASASNVVDPGGEVVVAATKPGLARIGADKCRVCHKVQHASWAA
                     SGHAKRTPPLDCEGCHGPGSEYKALAVMKDPKKARAAGLVDPDAAYCGKCHKGPWDPA
                     LLRKAHAHKG"
     gene            complement(995..2848)
                     /locus_tag="P5164_06795"
     CDS             complement(995..2848)
                     /locus_tag="P5164_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43640.1"
                     /translation="MLVSRAHAAPVALLLAALLAPRPLRGDDLLAQLGDAARNGRLEA
                     EAKLRAGSVPVLKGYSVTVKGAPATRLDVAVREGKLEALRYTAPDDSFLLVGKGLRPD
                     VVLTRVETDPTGTITTLDFHGRGLGRLVVGLFRPLVRKKVRQMKLHTELAELFRGNVV
                     VAASKPASASTPAASRAADASDATEPAPGAAATPSPPQPGFLDLVQEVRLLELRLSAY
                     PGKPLSFGETLVLETGGEDGSDPIEVSLDEALYRPGRDGAEAEWTAKGRLEGTLGAGS
                     VALEDGKVAFRSAALLGGRFAAEKKGDTPLSTSLRAGRFDLALARSDLRLPGGIDVAV
                     EDGSRFGVSSLVLDPTGAYRGRVDFRLLGGTGEIRHDGERLSLSGATISSGGLDVRDG
                     RATGPLSLDFDYHLVHPFVVKYPGDAQPPKTVPLDFRGPFSARLQLTDAGSGGGGSVT
                     GEYSLKIPWKPVEQAAIEALKARWTQDVKVVRKVDFTIEPRSFGPCGPQCFGASFAFT
                     AEKKRGERSLFRQLCEPEGKAELAVDPAARSFVLKNLQVKPRCTGAVGWFVNLVAPLL
                     TKTYSDMTLFQLPPDLPFSIETVRSGNDFVRIDGEIAWRGKSSPPAAPPLD"
     gene            2912..3703
                     /locus_tag="P5164_06800"
     CDS             2912..3703
                     /locus_tag="P5164_06800"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY43641.1"
                     /translation="MMRPETPAALRLLALHAFPLDGRLWGLVSAAAASGQLGDGVAVT
                     APDFRGRGKSGLPAEKVHGMPLLAKDAAELLRPDEPFVVMGMSMGGYVALELLARLGP
                     ELRPRLRGLVLCDSRANADDEAGKAKRTATADAIERDGMEPLLAAMLPQFLAPSSRGG
                     ALEQLIRTMFLATPPAAAAADQRGMAERADRFDVLGALAVPFLAAVGEDDPLTPPSAS
                     EAMVEAAGNSPYVELLTLPGAHMAPLEHPEEFLPKLRAFLDRCRP"
     gene            complement(3796..6330)
                     /locus_tag="P5164_06805"
     CDS             complement(3796..6330)
                     /locus_tag="P5164_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRY43642.1"
                     /translation="MTSRAIAVLGLLAVLVVPAAASGDALALLAELPSSGSPGDLSPR
                     RLEPGGPVFLVSVAANAIETWPAGAISEPVVSPGPGMAIRGFPAFGDFDGDGRIDVAV
                     SVATGIQVLAGDGNGRFRAAIGIPDDGARPLTAGDVDGDGRSDLIVGALSPGGVVLRT
                     FQSLGEFRFAPPRDTPGLPPGSDSGSFTAGDVDGDGRSDVVVASDGSASVWISRGDGT
                     FVAAPPLQEPQPADEAILADLDLDGFPDLLLAHQSLRYRTVVVLHNDGAGIFHRLPFS
                     EMSTFAERLTVGDFDGDGALDIATVPVTEAPCSYTLTVYPGDGRGGFGTARRLPVPCV
                     PGLGLITRVTAIDWDGDGTKEIAASASRGPVVLSPPRTAYDDVLFVPVLVSTSGVGGV
                     RFESDLLVTNSGATRVRLTLGYVATGGGGSGTVTADLEPGAQLHAPSALGWLRAAGLP
                     IAETGPSIGTLRIGVAGASALHAVRASVLTKSSAGGGTSTPALTRTEALRGNAIVPWL
                     TEAERDRTNLALANAGAAEDGPVTLRVTVASGDPAAPGLAELPDVELPPGAFFQLNRV
                     LLASRLPCRVAWARIRRVAGNAPYVAWATVNDAVTGDASIVAAVPEDLPEPWRFEFPA
                     AVQSERFATELVLTNPGESSARATFGLAGLGTFAQDLAPGEVFVVGDLFAEMRRRDPV
                     GALPAGVPFVGSLAVTGVLGGVRVTTTRGSARFGVFEPPAATEAASVVLPDLRRDSAT
                     RTNLAIANGYGFGGPHRFRLEVHDGETGRLAGSRDVTIERGARLQLDDVLQELDPPVS
                     RAWARIVPAYPTFFSAYAVVMDGAAPGEGSDDGAFIPGSPEETPAR"
     gene            complement(6411..7646)
                     /locus_tag="P5164_06810"
     CDS             complement(6411..7646)
                     /locus_tag="P5164_06810"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRY43643.1"
                     /translation="MRREAKGYRLLETSGVPVKAWTLGVSCDPAAERQLANAASLPIV
                     FRWIAAMPDVHVGIGATVGAVIPTVEAIIPAAVGVDIGCGMAASLTSLTANDLPSSLA
                     PLRAAIETAVPHGRTDSGGRNDRGAWHDPPARIGEAWRALEPGWRAILDARPELDRGN
                     HPARHLGTLGTGNHFIEVCLDEADRVWLLLHSGSRGVGNRIGTDYIEIAREEMRVHQR
                     NLPDRDLAWLTEGTESFDDYAAAVSWAQEYARTNRELMLVAIEEALASVPSLPPFATR
                     VLAVSCHHNYVRRERHYGREVWITRKGAVRAGKGELGIVPGSMGARSFIVRGLGNPES
                     FESCSHGAGRLLSRTEARRRFTLADHAEATAGVECRKDEAVLDETPAAYKPIDAVMNA
                     QKDLVEVLHTLKQVVCVKG"
     gene            7726..10980
                     /locus_tag="P5164_06815"
     CDS             7726..10980
                     /locus_tag="P5164_06815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRY43644.1"
                     /translation="MLRTGTVLLAVCLLSPGAASALDPGKRLSQYGLDVWQEGDGLPQ
                     NTVQAVLRGRDGYLWLGTQEGLVRFDGARFTTFDRRNTPELKHNSVISLAEGPDGTIW
                     AGTNGGGVLCRRRDGTFSTIGTAEGLAGGIVWSIAFDRAGDAWVAGDGIGAQRVVDGR
                     GAERIGLSQGLPLEQVRAIAFDASGALWIGTTGAGAAIVKNGRVTKVWDAAALGTGLV
                     RGLAPDAAGGVWVASSGGGLTYLKGDTVVRYRAADGFPSDQVSAVHVDARGTLWFGTW
                     GAGVGRLRDGVPETIGTREGLSNDQVWSLTSDEEGSVWIGTWVGGLNRLRDGLFLTYT
                     TREGLTSDNVRAVCPGRDGSVWIATAGGGLNRLKGGRVTAYREADGLPSDHVSALLED
                     RRGRLWVGTNLAGAAVLERGRFHPLGPAQGLPHHDVRAFLEDRDGNVWIGTIGGGLTR
                     VAPDGSLRVFTVEQDGLPTDRVVALAEAPDGALWIGSSGGGAVRYAEGRFRAFTMRDG
                     LTSNRVLTVFPDAGGAVWAGTSGGGLNRIRGDAVTPVTTRDGLFDDLVQVILDDGKGA
                     LWMTCNKGVFRVDRAELEAVADGRKATLRSFALGASDGMRSASAAGGQQPAGFVADDG
                     RLWFPTFRGVAVLDPARIPASGRTPPVAIEEVLVDGRPVDLRVPLVLPPRAERVEIHY
                     TALTLVAPDRVRFRIRLDGLDEEPLEVGTRRVAYYTNLPSGPYVFRVSAADSGGAWLG
                     QEATFSFRKRARAREVWWFWVGLLGAASGATWGGVKWRLASLRARESELLALVAERTR
                     SLSEEKTRAEEALGRAEEARAEAERLREVAEKATAEAEEASRTKSRFLANVSHELRTP
                     LNAIIGYSEMLSEEASDEGRQALKRDLEKIRSAALHQLELVNSVLDLARIEAGRLDLD
                     VETFPVRPLVEETAAIVRPLLEKNRNRLSVSFSPEGAFEMTSDAMKVRQCLFNLLSNA
                     CKFTCDGRVEVDVSLVEEEGRPWASFRVRDTGIGITPEKAARLFEPFAQADAATAQRF
                     GGSGLGLSITRELCLLMGGRVTVESVPDEGSVFEIRLPAALPLPEAVAGPPGEP"
     gene            complement(10984..>11397)
                     /locus_tag="P5164_06820"
     CDS             complement(10984..>11397)
                     /locus_tag="P5164_06820"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRY43645.1"
                     /translation="IASTSGSSAGIGFAVPVDTVNQVVPQLIQHGRVIRPRLGVTLAD
                     DAVAARLGVEGALILSVEPETGAAEAGLRGTTRDEDGSLLLGDVVTRAGERPIRSADD
                     LIAALEEKKPGETLPLLVLRDGETVRVAVSLSAAP"
BASE COUNT         1514 a         3940 c         4450 g         1493 t
ORIGIN      
        1 agcgttagac tccggatcta aaggtcccgc gttcgaatcg cggcgggcgc accactcttt
       61 cgacgatggc cgcggagacg cggcctttcg cgttccgggc cgcggaaggg ctcggggaac
      121 ccgggcgcgg gttccccgga aacaggcccg accgttagac gcaggtacta aaggtcccgc
      181 gttcgaatcg cggcgggcgc accactcttt cgacgatggc cgcccagacg cggcctttcg
      241 cgttctaccc cttgtgcgcg tgggccttcc ggaggagcgc ggggtcccag gggcccttgt
      301 ggcacttgcc gcagtaggcg gcgtcgggat cgacgaggcc ggcggcgcgg gccttcttcg
      361 ggtccttcat cacggcgagc gccttgtact cgctgccggg gccgtgacat ccctcgcagt
      421 cgaggggcgg cgtccgcttc gcgtggcccg aggcggccca ggaggcgtgc tgcaccttgt
      481 ggcagacgcg gcacttgtcg gcgccgatgc gcgcgaggcc gggcttcgtc gcggcgacga
      541 cgacctcgcc gcccgggtcg acgacgttcg aggcgctcgc ggcgggggcc gccgtcggct
      601 tcggcggcgt cgtcgggacg acggtcgggg gcggcacgac ggtcgggggc gcggcgggcg
      661 gcgccgtcgg cgcggccttc gggggaacgg tcgtcggctt cggtttcgcg gggacgatcg
      721 tcggcgccgc cgggggcgcg gcgggcgtag cgatttcagg aggtgtcggg gccgtctcgg
      781 cctcgggcgg agcgggcgac gtcggaggcg gggcggcctc gatcggcgcg accggcgcgg
      841 gcggctccgg ggccgggggc gccgcctctc gcgccttgca ggcggcgaag gagagggcga
      901 agagggcgag cgcgaggcgt gctgttctca tggctcctcc gtcccggact tcggttcgtg
      961 aggcaagctt agccccgggg ggaagtcgcc gcgctcagtc gagcggcggg gcggccggcg
     1021 ggctgctctt tcctcgccac gcgatctcgc cgtcgatccg gacgaagtcg ttcccgctcc
     1081 tcaccgtctc gatcgagaac gggaggtcgg gcgggagctg gaagagggtc atgtcggagt
     1141 aggtcttcgt cagcagcggc gcgacgaggt tcacgaacca gccgaccgcc cccgtgcacc
     1201 gcggcttcac ctggaggttc ttgaggacga aggagcgggc ggccgggtcg acggcgagct
     1261 cggccttccc ctcgggctcg cagagctgcc ggaagaggct ccgctcgccg cgcttcttct
     1321 cggcggtgaa ggcgaagctg gccccgaagc actgcgggcc gcacgggccg aaggagcgcg
     1381 gctcgatcgt gaaatcgacc ttccggacga ccttcacgtc ctgggtccac cgcgccttca
     1441 gggcctcgat cgcggcctgc tcgaccggct tccagggaat cttcagcgag tactcgccgg
     1501 tgacgctccc gccgccgccc gacccggcgt cggtgagctg caggcgtgcg gagaagggac
     1561 cgcggaagtc gagaggcacc gtcttcgggg gctgggcgtc gcccgggtac ttcacgacga
     1621 acgggtggac gaggtggtag tcgaagtcga gggagagggg gcccgtcgca cgtccgtccc
     1681 ggacgtcgag gccgccggac gagatcgtgg cgcccgaaag ggagagccgc tccccgtcgt
     1741 ggcggatctc gccggttcct ccgaggaggc ggaagtcgac gcggccgcgg taggcgccgg
     1801 tcgggtcgag gacgagggag gagacgccga agcgcgagcc gtcctcgacg gcgacgtcga
     1861 taccgcccgg caggcggagg tcggaccggg cgagcgcgag gtcgaagcgg ccggcacgga
     1921 ggctcgtcga gaggggcgtg tcgcccttct tctccgcggc gaagcggccg ccgaggagcg
     1981 cggccgaccg gaacgcgacc ttcccgtcct cgagcgcgac ggagcccgcg ccgagcgtcc
     2041 cctcgagccg ccccttcgcc gtccattccg cctcggcgcc gtcgcggccg gggcggtaga
     2101 gcgcctcgtc gaggctcacc tcgatcgggt cgctcccgtc ttcgcccccc gtctcgagga
     2161 cgagcgtctc gccgaacgag agcggtttcc ccgggtaggc cgagagccgg agctcgagga
     2221 gccggacctc ctggacgagg tcgaggaagc cgggctgcgg aggggagggc gtcgcggcgg
     2281 cgcccggggc cggctcggtc gcgtcggagg cgtccgccgc acgggaagcg gccggggtgc
     2341 tcgccgacgc cggcttcgac gcggcgacga cgacgttccc gcggaagagc tcggcgagct
     2401 cggtgtggag cttcatctgc cggaccttct tccgcacgag cggccggaaa aggccgacga
     2461 cgagcctgcc gaggccacgc ccgtggaagt cgagcgtcgt gatcgttccg gtgggatcgg
     2521 tctcgacccg ggtcaggacg acgtcgggcc gaagcccctt ccccacgaga aggaagctgt
     2581 catcgggcgc cgtgtagcgg agcgcctcga gcttcccctc ccggacggcg acgtcgaggc
     2641 gtgtggccgg ggcgcccttc acggtgaccg agtagccctt caggaccggg acggagcccg
     2701 cgcggagctt cgcctcggcc tcgagccggc cgttccgggc ggcgtcgccg agctgggcga
     2761 ggaggtcatc gccacggagc ggccggggcg cgaggagcgc ggcgaggagg agcgcgacgg
     2821 gagcggcgtg ggcgcgggaa acgagcacgg gacctccggg gggatcgtga tgtcgggacg
     2881 gccgcggagc ggcgcgggct atcctcgggg aatgatgcgc cccgagacgc ccgccgcgct
     2941 ccgcctcctc gccctccacg cgttccccct cgacggccgg ctctggggcc tcgtctcggc
     3001 cgcggccgcg tcgggccagc tcggcgacgg cgtcgccgtc acggcgcccg acttccgcgg
     3061 gcgagggaag tccggcctgc cggcggagaa agtccacggg atgccgctcc tcgcgaagga
     3121 tgcggcggag ctgctccggc ccgacgagcc gttcgtcgtc atggggatgt cgatgggggg
     3181 gtacgtcgcg ctcgagctcc tggcccgcct cggaccggag ctgcggccgc gcctccgcgg
     3241 cctcgtcctc tgcgactcgc gcgcgaacgc ggacgacgag gcggggaagg cgaagcggac
     3301 ggcgacggcg gacgcgatcg agagggacgg gatggagccc ctcctcgcgg cgatgctccc
     3361 gcagttcctc gccccctcct cgcgcggcgg cgcgctcgag cagctgatcc ggacgatgtt
     3421 cctcgcgacg ccgccggcgg cggccgccgc cgaccagcgc ggcatggcgg agcgcgccga
     3481 ccgcttcgac gtcctcggcg cgctcgccgt cccgttcctc gcggcggtcg gcgaggacga
     3541 cccgctcacg ccgccgtccg cctccgaggc gatggtcgag gcggcgggga acagccccta
     3601 cgtcgagctc ctgacgctcc ccggggccca catggctccg ctcgagcacc ccgaggagtt
     3661 cctcccgaag ctccgggcgt tcctggaccg ctgccggccg taaacccgcc ccgccccccc
     3721 acccaccccc cttcgcggtg cccgcgtccg cggcgcggga gctgccaatg aagcgggcgc
     3781 ggggtcgact ccagcctagc gcgctggggt ttcttcggga gatccgggga tgaaggcgcc
     3841 gtcgtcgctc ccctctcccg gcgccgcgcc gtccatgacg accgcgtagg cgctgaagaa
     3901 ggtcgggtag gccgggacga tccgggccca ggcgcgcgag acgggcgggt cgagttcctg
     3961 caggacgtcg tcgagctgaa gccgcgcgcc gcgctcgatg gtcacgtcgc gcgacccggc
     4021 gagacggccg gtctctccgt cgtggacctc gagccggaag cgatgggggc cgccgaaacc
     4081 atatccgttc gcgatcgcga ggttggtccg cgtggcgctg tcccgccgca ggtccggcaa
     4141 gaccaccgag gcggcttccg tggcggcagg cggctcgaag acgccaaagc gcgcgctccc
     4201 gcgcgtcgtc gtgacacgaa cgccgccgag gacgccggtg acggcgagcg agccgacgaa
     4261 cgggacgccc gcaggaagcg caccgaccgg atcccgacgc ctcatctcgg cgaagaggtc
     4321 gccgacgacg aacacctccc ccggggcgag atcctgcgca aaggttccca gccccgcgag
     4381 gccgaacgtc gctcgggcgg aactttcgcc gggattcgtg agcaccagct cggtcgcgaa
     4441 gcgctcgctc tgaacggcgg ccgggaactc gaagcgccag ggttccggaa gatcctccgg
     4501 gacggcggcc acgatcgagg cgtcaccggt gacggcgtcg ttcacggtgg cccaggcgac
     4561 gtagggcgcg ttccccgcga cgcgccggat ccgggcccac gcgacgcggc aggggagtcg
     4621 tgaggcgagc aggacgcggt tgagctggaa gaaggcgccg ggcggcagct ccacgtcggg
     4681 gagttctgca agacccggtg cggcagggtc gccggaagcg acggtcacgc gaagcgtgac
     4741 cggcccgtct tccgccgcgc cggcgttggc gagcgcgagg ttcgtccggt cgcgctccgc
     4801 ctccgtcagc cacgggacga tcgcgttccc gcgaagggct tccgttctcg tcagggccgg
     4861 agtggacgtg ccccctcccg cgctcgactt cgtgaggacc gacgcgcgaa cggcgtggag
     4921 ggccgaagcg ccggcgaccc cgattcggag ggtgccgatc gagggtcccg tctccgcgat
     4981 cggaagcccg gcggcgcgca gccagccgag tgcgctcggc gcgtgaagct gagcgcccgg
     5041 ctcgaggtcg gcggtgacgg tcccgctccc gccgcctccg gtggcgacgt agccgagggt
     5101 gagccggacg cgtgtcgccc ccgagttcgt cacgagcagg tccgattcga agcggacgcc
     5161 cccgaccccc gacgtcgaga caaggacggg aacgaagagg acgtcgtcgt acgccgttcg
     5221 cggaggggac aggacgaccg ggccgcgcga cgcgctcgcg gcgatctcct tcgtcccgtc
     5281 tccgtcccag tcgatcgccg tcacgcgagt gatcaggccg agcccgggca cgcaggggac
     5341 agggaggcgc cgggccgtcc cgaagccgcc tcgtccgtcg ccggggtaga cggtgagcgt
     5401 gtagctgcag ggcgcctccg tgacgggaac ggtggcgatg tcgagggccc cgtctccgtc
     5461 gaagtctccg acggtcaggc gctcagcgaa ggtcgacatt tccgaaaagg ggagccggtg
     5521 gaagattccg gccccgtcgt tatggagcac gacgacggtc ctgtaccgaa gggactgatg
     5581 cgcgagcagg agatccggga atccgtcgag gtcgaggtcc gcgaggatcg cctcgtccgc
     5641 cggctgcggc tcctgaagcg gcggcgcggc gacgaaggtc ccgtcgccgc ggctgatcca
     5701 cacggacgcg ctgccgtcgc tggcgacgac gacgtcggag cgtccgtccc cgtcgacgtc
     5761 tccggccgtg aaggaaccgc tgtcgctccc cggcggcaac ccgggcgtgt cccgcggggg
     5821 cgcgaagcgg aactccccga gcgattggaa cgtccggagg acgaccccgc ccggcgagag
     5881 cgcgccgacg atcaggtcgg accggccatc tccgtcgacg tcgcccgccg tgaggggcct
     5941 ggctccgtcg tccggaattc cgatggcggc acggaaacgg ccgttgccgt ccccggcgag
     6001 cacctggatg cccgtcgcca cggaaacggc gacgtcgatc cgaccgtcgc cgtcgaagtc
     6061 cccgaaggcc gggaatcccc ggatcgccat tccgggcccc ggcgagacga caggctccga
     6121 gatcgcgccg gccggccacg tctcgatcgc gttcgcggcg acggagacga gaaagaccgg
     6181 cccgcccggc tcgaggcgtc ggggcgatag gtcgccgggc gaccccgagg acggaagctc
     6241 ggcgagaagg gcgagcgcgt cgccggacgc ggcggcggga accacgagga cggcgaggag
     6301 cccgagcacg gcgatcgcgc gtgaggtcat ccgctggaag tgttgcggcg ggaatccgcc
     6361 tcacggcgga acaagggagc ctcggcgggc cctcgtgcga gacgacggac ctaccccttc
     6421 acgcagacga cctgcttgag cgtgtggagg acctcgacga ggtccttctg cgcgttcatc
     6481 acggcgtcga tcggcttgta ggcggcgggc gtctcgtcga ggacggcctc gtccttgcgg
     6541 cactcgacgc cggcggtcgc ctcggcgtgg tcggcgagcg tgaagcggcg ccgcgcctcg
     6601 gtgcgcgaga ggagccgccc ggcgccgtgg ctgcacgact cgaagctctc ggggttgccg
     6661 aggccgcgga cgatgaacga gcgggcgccc atgctgccgg ggacgatccc gagctctccc
     6721 ttcccggcgc ggaccgcgcc cttgcgcgtg atccagacct cgcggccgta gtgccgctcg
     6781 cgccgcacgt agttgtggtg gcaggagacg gcgaggacgc gcgtcgcgaa cggcgggagc
     6841 gacgggaccg acgcgagcgc ctcctcgatc gcgacgagca tgagctcgcg gttcgtccgc
     6901 gcgtactcct gcgcccagga gaccgcggcc gcgtagtcgt cgaagctctc cgtcccctcg
     6961 gtgagccagg cgaggtcgcg gtcggggagg ttccgctggt ggacgcgcat ctcctcgcgg
     7021 gcgatctcga tgtagtcggt gccgatccgg ttcccgacgc cgcgcgagcc gctgtggagg
     7081 aggagccaga cgcggtcggc ctcgtcgagg cagacctcga tgaagtggtt cccggtgccg
     7141 agcgtcccga ggtggcgcgc ggggtggttg ccgcggtcga gctcgggacg ggcgtcgagg
     7201 atcgcccgcc agcccggctc gagcgcgcgc cacgcctcgc cgatgcgcgc gggcgggtcg
     7261 tgccaggcgc cccggtcgtt ccgaccgccg gagtcggtgc gcccgtgggg gacggccgtc
     7321 tcgatcgccg cgcggagcgg cgcgaggctc gaggggaggt cgttcgcggt gagcgacgtg
     7381 agggacgccg ccatgccgca gccgatgtcg acgccgacgg cggccggaat gatcgcctcg
     7441 acggtcggga tgacggcgcc gacggtggca ccgatcccga cgtggacgtc gggcatcgcg
     7501 gcgatccagc ggaagacgat cgggagagag gcggcgttcg cgagctgcct ctcggcggcg
     7561 gggtcgcagg agacgccgag cgtccacgcc ttcacgggga ctccgctcgt ctcgaggagc
     7621 cggtagccct ttgcctcgcg cctcacgccc cgaacatgcg gacggagaac gggatcgtca
     7681 agcgccttcc ggcggtggct cgggatgcgc gcctatgatc ccggcatgct gcggaccggg
     7741 acggtgctgc tcgccgtctg cctgctctcc cccggagccg cttcggcgct cgatccgggc
     7801 aagcgcctgt cgcagtacgg cctcgacgtc tggcaggagg gagacggcct gcctcagaac
     7861 accgtgcagg cggtcctccg cggccgcgac ggatacctct ggctcggcac gcaggagggg
     7921 ctcgtccggt tcgacggggc ccgcttcacg acgttcgacc ggcggaacac cccggagctg
     7981 aagcacaact ccgtcatctc gctcgcggag ggtcccgacg ggacgatctg ggccggcacg
     8041 aacggcggcg gcgtcctctg ccgccgccgc gacgggacct tctcgacgat cggcacggcc
     8101 gaagggctcg cgggcgggat cgtctggtcg atcgcgttcg accgcgcggg ggacgcctgg
     8161 gtggcggggg acgggatcgg cgcccaaagg gtcgtcgacg gccggggagc ggaacggatc
     8221 ggactgtcgc aagggctccc gctcgagcag gtccgggcga tcgcgttcga cgcgagcggc
     8281 gcgctctgga tcggcacgac cggggccggc gcagcgatcg tgaagaacgg ccgggtcacg
     8341 aaggtctggg acgccgcggc gctcggcacg gggctcgtgc gcgggctcgc gcccgacgcc
     8401 gcgggcgggg tctgggtcgc gtcgtcgggg ggcgggctca cgtacctgaa aggcgacacc
     8461 gtcgtgcggt accgcgcggc cgacggattc ccgtccgacc aggtctccgc ggtccacgtc
     8521 gacgcgcgag ggacgctctg gttcggcacc tggggcgccg gcgtcgggcg gcttcgggac
     8581 ggggtcccgg agacgatcgg gacgcgcgag ggcctctcga acgaccaggt ctggtccctc
     8641 acgagcgacg aggaggggag cgtctggatc gggacgtggg tgggtggcct gaaccgcctg
     8701 cgcgacggcc tcttcctgac ttacacgacg cgcgaggggc tgacgagcga caacgtccga
     8761 gccgtctgcc cggggcgcga cggctccgtc tggatcgcga ccgccggcgg cggcctgaac
     8821 cggctgaagg gggggcgcgt cacggcctac cgcgaagcgg acggcctgcc gagcgaccac
     8881 gtgtcggcgc tcctcgagga ccggcgcggc cgcctctggg tcgggacgaa cctcgcgggg
     8941 gccgcggtcc tcgagcgggg ccgtttccat ccgctcggac cggcgcaggg gctgccgcat
     9001 cacgacgtga gggcgttcct cgaggaccgc gacgggaacg tctggatcgg gacgatcggc
     9061 ggcggcctga cccgcgtcgc cccggacggg tcgctccgcg tcttcacggt cgagcaggac
     9121 ggcctgccga ccgaccgcgt cgtggccctc gccgaggctc cggacggggc gctctggatc
     9181 ggttcgtccg gcggaggggc cgtccgctac gcggaagggc gcttccgtgc gttcacgatg
     9241 cgggacggcc tgacgagcaa ccgggtcctc accgtctttc ccgacgcggg cggcgcggtg
     9301 tgggcgggga cgtcgggcgg cgggctgaac cggatccgcg gcgacgcggt cacgcccgtg
     9361 acgacgcgcg acgggctctt cgacgacctc gtccaggtga tcctggacga cgggaagggc
     9421 gccctctgga tgacgtgcaa caagggggtc ttccgcgtcg accgcgccga gctcgaggcc
     9481 gtcgccgacg gccggaaggc gacgctccgc tcgttcgcgc tcggcgcgtc ggacgggatg
     9541 cggagcgcga gcgcggcggg agggcagcag cccgcgggat tcgtcgccga cgacgggcgc
     9601 ctctggttcc cgacgttccg gggggtggcg gtcctcgacc cggcccgcat cccggcctcg
     9661 gggcggacgc cgccggtcgc gatcgaggag gtcctggtcg acgggcgacc cgtggacctc
     9721 cgcgtacctc tcgtcctccc tccgcgggcc gagcgggtcg agatccacta cacggcgctc
     9781 acgctcgtgg cgcccgaccg ggtccgtttc cggatccgcc tcgacggcct cgacgaggag
     9841 ccgctggagg tcgggacgcg tcgcgtggcg tattacacga acctcccgag cggcccgtac
     9901 gtcttccgcg tctcggcggc cgactccggc ggcgcgtggc tcggtcagga ggcgacgttc
     9961 tcgttccgga agcgggcccg ggcgcgcgag gtctggtggt tctgggtagg cctgctcggc
    10021 gccgcgtcgg gggcgacgtg ggggggcgtg aagtggaggc tcgcgagcct gcgcgcgcga
    10081 gagtccgagc tcctcgccct cgtcgcggag cggacgcgga gcctgtcgga ggagaagacg
    10141 cgcgccgagg aggcgctcgg gcgggccgag gaggcgcgcg cggaggccga gcgcctccgc
    10201 gaggtcgccg agaaggcgac ggcggaggcg gaggaggcga gccggacgaa gagccgcttc
    10261 ctcgcgaacg tctcgcacga gctgaggact ccgctgaacg cgatcatcgg ctactccgag
    10321 atgctctcgg aggaggcgtc ggacgagggg cggcaggcgc tcaagcgcga cctcgagaag
    10381 atccgctccg cggcgctcca tcagctggag ctggtcaact cggtcctcga cctggcccgg
    10441 atcgaggccg gacggctgga cctcgacgtg gagacgttcc cggtccggcc gctcgtggag
    10501 gagacggcgg cgatcgtccg gccgctcctc gagaagaacc ggaaccgcct ctccgtgtcg
    10561 ttctcgcccg agggggcctt cgagatgacg tcggacgcga tgaaggtacg gcagtgcctc
    10621 ttcaacctcc tgtcgaacgc ctgcaagttc acgtgcgacg gacgcgtgga ggtcgacgtt
    10681 tcgctcgtcg aggaggaggg ccggccgtgg gcctccttcc gcgtccgcga cacgggcatc
    10741 gggatcacgc cggagaaggc ggcgcgcctc ttcgagccgt tcgctcaggc cgacgcggcg
    10801 accgcgcaga ggttcggagg gagcggcctc ggcctgtcga tcacgcgcga gctctgcctc
    10861 ctgatgggag ggcgggtgac cgtggagagc gtcccggacg agggctcggt cttcgagatc
    10921 cgcctcccgg cggcgctccc cctgccggag gccgtcgccg gaccgccggg cgagccgtag
    10981 acgtcagggc gcggccgaga gggagacggc gacgcggacc gtctccccgt cccgcaggac
    11041 gaggaggggc agcgtctctc ccggcttctt ctcctcgagc gcggcgatca ggtcgtccgc
    11101 cgagcggatc gggcgctcgc cggcccgggt gacgacgtcg ccgaggagga gcgagccgtc
    11161 ctcgtcccgc gtcgtcccgc gcaggcccgc ctcagcggcg ccggtctccg gctcgacgga
    11221 gaggatgagc gcgccctcga cgccgagccg ggccgcgacg gcgtcgtcgg cgagcgtgac
    11281 gccgaggcgc ggccggatga cgcggccgtg ctggatgagc tgcgggacga cctggttcac
    11341 ggtgtcgacc gggaccgcga agccgatccc ggcgctcgag ccgctcgtcg aggcgat
//
LOCUS       DAOTEZ010000094        11355 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4016_length_11355_cov_6.702743, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000094 DAOTEZ010000000
VERSION     DAOTEZ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11355
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4016_length_11355_cov_6.702743"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="P5164_06825"
     CDS             complement(<1..1094)
                     /locus_tag="P5164_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HRY43646.1"
                     /translation="MRPSRREAIAALAGLPALSSLLAACRGGPDAPAFSGAIVGADVL
                     RGHRIRTGELLSRPVARKESVGVAILGAGISGLSAAWTFRRAGFTDFRIYELEDEPGG
                     NARSGANAVSAYPWGAHYVPVPMYGNAALETLLEEIGAVAGRSADGAPEWAEEMLCAE
                     PEERLYFRGLWYEGLYPRAGASREDREELARFEREMHRFAGLRDAKGRRAFALPRRRS
                     SDDAEWTALDRISMREWLTRGGYRGARLFWFAEYATRDDFGSSLDDTSAWAGIHYFAS
                     RLRGTASDGPFEEPASFLTWPEGNGRLVKHLSKAAGGRIVTAAVVFDVVPRPGGATLR
                     WLDAPRNEVVEVAARHVVYALPRYTAAKVLA"
     gene            complement(1096..2610)
                     /locus_tag="P5164_06830"
     CDS             complement(1096..2610)
                     /locus_tag="P5164_06830"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005037368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HRY43647.1"
                     /translation="MTPSPVRPGPVYLTVLVIATCGLVYELVAGALASYLLGDTVTQF
                     SLVIGIYLTAMGAGAWLSKFIEKGVARRFVEIEIAVAFLGGFSALILSFAFLSPRWFH
                     PVFYGLVFAVGTLVGVEIPLMLRLLRRSVAFKELVAQVLTFDYLGALAASLLFPLVFV
                     PTLGLFRTSLLFGLLNVAVAFTSIRLFRDEIGPAGGLYAKAGLVAALLLGGFVAADRL
                     SGLAEEQQYADEVLLAKSSPYQRVVLTRNRAGFQLFLNGHLQFSSVDEYRYHEALVHP
                     AFAVVPEARRIAVLGGGDGLAVREVLRHPSVEEVVLVDLDPLVTRLARENPLFRELNG
                     GSLLSPKVSVVNDDAMKWLETETGLFDLVFVDFPDPNSFAVGKLYTKRFYGLLKRRLA
                     PDGAFAVQSTSPLFARRSFWIIDRTIAATGFETRAYHAAVPSFGEWGFVLASPRPFDV
                     PSDVLPGLRFLSRETLPALFSFGPDMAPWAEAPVNRLHDQVLVRTYESEWRAFE"
     gene            complement(2615..2836)
                     /locus_tag="P5164_06835"
     CDS             complement(2615..2836)
                     /locus_tag="P5164_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HRY43648.1"
                     /translation="MNWWQLHAGQVWSALVFSAIGIAVFGLFFLVLPRILPFSLKKEI
                     EEDQNVALGIVIAAVVLGMAFIIGSAIGS"
     gene            complement(2836..3006)
                     /locus_tag="P5164_06840"
     CDS             complement(2836..3006)
                     /locus_tag="P5164_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43649.1"
                     /translation="MLKRGFQVFGALLLTAYAWAGLAGWELPTNAKKGVVAAGARSAT
                     GFRAYSFWTGGK"
     gene            complement(3014..4996)
                     /locus_tag="P5164_06845"
     CDS             complement(3014..4996)
                     /locus_tag="P5164_06845"
                     /inference="COORDINATES: protein motif:HMM:NF025158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HRY43650.1"
                     /translation="MSSRAASCPSCGGEVLFRAGSSVVTVCPQCRSAVSRKGANLEAL
                     GVVAELVPTSSPFRVGMTGRPKAAGLKPFRIVGRLQLSTGEGTWDEWHVSFEDGRYGW
                     LAEAQGTFWVTRPLPPPTNPPPPDFPQIAPGQRLSFGGYGEFTVTDRRQALYASAEGD
                     LPFAATPGAVFMYADLSGADGSVGTLDYGDDPGVDAFFVGRSVALADLGVEGLEGWSE
                     RKVAARASSLNCPACGAGLQLKDPANTVRVACTYCGSVLATPREGASAEKFEVLARLQ
                     KLPFKPLLPLGGEGTLRGRPYAILGAVLKACTVEGVHYFWREYLLKETKSEAYHWLVE
                     SNGHWTLVEPAAAGEVSTAPRLAVWRGTRYRHFQSTTARVEAVLGEFYWEVKKGESTD
                     VSDYVAPPRILSEERYANEVTWSEGSYVPKEELESGFRLGAPLPAPEGVGSNQPWPHA
                     ETARGFLRTVALFAGIAVFLFVFFNIKADRKVVYQKRHDLSTAATDPTIAPDAVAEAL
                     TVVTEPFEVPHAGNVEARIDAPTDNSWVYVGAVLLEEATGQAYGFGLQSDYYHGVDGG
                     ERWSEGSRSRTVYVGRVPAGRYVLRLEPELERGKAPPYYDIRLRSGVPRFAHLMAVLL
                     LLLLGPIALAISKARFEGRRWAESDYGGGSGSDDDE"
     gene            complement(5079..6443)
                     /locus_tag="P5164_06850"
     CDS             complement(5079..6443)
                     /locus_tag="P5164_06850"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRY43651.1"
                     /translation="MSERDLDDAWKLRFRDPLVEAAFREEQRSSSARILRPSVFVALA
                     TILAFGVLDPIAFPETWRSVLVIRLVALALPVAVLAFLASLPRFARWTRPLLEVGSVA
                     AGWAVAAMIAVGQPQEPGTTVYFAALSFPLASAAIGLLPSGFAASVVRAGLIVLAFPA
                     VALVRKLPGWGPPLPADVVVVSLGTLLLLWFFFSVAGYFREAWQRRTFVTMRLLEAAS
                     EKVRSVAADLKRMNEEKSTFLGIAAHDLRNPLGVILGYAEMLRDEEFPRREAADMAAK
                     IVVSAERVRDLVAGLLEVSAAEAGRVALERRPCDLLAISRRVVETQQEPAARKEIGIR
                     LDGSPVVVTGDPGAIQQVIENVVSNAVKFSPWRSTVVVSVHADGGAGLVDVEDEGPGV
                     SDADRARLFAKFARLSARPTGGESSTGLGLSIVKTLTEAMGGEVSLESPPGRGARFRV
                     KLPV"
     gene            complement(6589..7575)
                     /locus_tag="P5164_06855"
     CDS             complement(6589..7575)
                     /locus_tag="P5164_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRY43652.1"
                     /translation="MTYIVLIVVVALLFDYINGFHDAANSIATVVGTRVLSPRVAVVW
                     AAFFNFVAAFALETRVAQTIGKGIVRSDVVDPDLVLASLIGAIVWNLLTWWWGLPSSS
                     SHALIGGFAGAAFVKAGAGSLVLKGLLVTSAFIVLSPLLGLALGFLNFLGAQWAFRRW
                     RPDRVDRVFRKVQLLSAAAFSFGHGMNDAQKTMGIIFALLLSTGHLTGNHIPFWIVLL
                     CFSAIGLGTLSGGWRIVRTMSMRITPLKPIGGSCAELAGAVTLVGASLGGIPVSTTHT
                     ITGAIMGVGATRGLSSVRWGVARSIVVAWVLTIPASALVAGLTWKLFDLLRG"
     gene            complement(7582..8202)
                     /locus_tag="P5164_06860"
     CDS             complement(7582..8202)
                     /locus_tag="P5164_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HRY43653.1"
                     /translation="MRLFPREETFLPQFARGAEVILEAARVLDALAAAPVLDDERAVR
                     IKQLEHQGDQIAHETFDLLNRTWITPIDREDIHVLVRNLDDVLDFIDAAAARIVLYRI
                     ASTTPELKKITSVIVQSAEAIQKAMALLSDLKHSKQILEACVEINRLENEADTVAQLA
                     IGRLFAEARDPIEVMKWKEIYDFVEGATDRCEDVANTLEAIVIKST"
     gene            complement(8273..9382)
                     /locus_tag="P5164_06865"
     CDS             complement(8273..9382)
                     /locus_tag="P5164_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRY43654.1"
                     /translation="MTTPDPPRPPLRAPVLARRLVLPLLLGLAGGLVAGAILGRILDG
                     AVAGRTKETAFSLLERVGPDVEAVLATGGDPKETVDRIGRQLALRATLVATDGRVLGD
                     SGVDRSRLDALENHAGRPEVRDALRSGRGVEARFSSTLGQRLVYAAARLRSGEVLRLA
                     FPEKDLTAWEAPYRRRVLLVCVAAGLLAGALFVRLRLRQAAELGHVLRAVEAVARGRR
                     PANPGAVLEETAAVHSALADLLEQTSERNEAVLRQETVSRAIFEGIPTGLLVVDRELS
                     LLDANSAALGLLRLPAGALRRGEHVLELVREKAVTDLLREALDGGRAAGPLRLATGSG
                     DLELRAEAIRVSGEGAAGRPAAVAILREAASPRPS"
     gene            complement(9411..10976)
                     /locus_tag="P5164_06870"
     CDS             complement(9411..10976)
                     /locus_tag="P5164_06870"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRY43655.1"
                     /translation="MNLDTFRTQFFAGAPRYTWLEDLGRGGMGVVFKARDRDLDEVVA
                     IKVLYGHVGDDDGAILARFKREISLNRRVKHPNVARMYDFGSGGGHPYITMEFVPGQD
                     LQSLILQEGRIPPERAIRILRQICQGTQAAHEQEIVHRDLKSQNIIVGAGDVVSILDF
                     GLARGAVDEKLTQEAVVLGTPHYISPEQAMGQSADVRSDIYSIGIIAFEMLSGVLPFT
                     ADSALGIAMKQISEPVPGNLSLYPEIPQRLREVVHRALAKRREDRPPTAAALDAALAR
                     AAGQPDSPEVDDEAAALARAIDAALGDPGGGPRPQRPVDALRDDPTPVVPHRPRPRPA
                     PEAEPAPARILTPSPSMRSPSGVATPPSVPAVRRPAGRPTVFVVMGDGADRIAAGKAF
                     ADSGCDAVEARSGEEILELLMSRVPDAVVMDIALPKADGFEVARILKATPTFAQVPVL
                     LLGTRVDRAQEHFARQVGASEILSRPVSDKELVERTWRLLAPRGYYRDEAPSSRISRS
                     TAPVPPPSDPTRS"
     gene            11135..>11355
                     /locus_tag="P5164_06875"
     CDS             11135..>11355
                     /locus_tag="P5164_06875"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141027.1"
                     /note="catalyzes the formation of glycerone phosphate and
                     D-glyceraldehyde 3-phosphate from D-fructose
                     1,6-bisphosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HRY43656.1"
                     /translation="MTTIDTITTALGAEAKSLLEHTCSGIPKETLHLPGPDVVDRIWS
                     MSDRPIPVLRSLQQLVDHGRLAGTGYLSIL"
BASE COUNT         1732 a         3905 c         4306 g         1412 t
ORIGIN      
        1 gcgaggacct tcgccgcggt gtagcggggg agcgcgtaga cgacgtgccg ggccgcgacc
       61 tcgacgacct cgttccgggg cgcgtcgagc cagcggagcg tggcgccgcc agggcgcggg
      121 acgacgtcga agacgacggc cgccgtgacg atccgtcccc cggccgcctt cgagaggtgc
      181 ttcacgaggc gcccgttccc ctcgggccag gtgaggaacg aggcgggctc ctcgaacgga
      241 ccgtccgagg cggttccccg gaggcgcgag gcgaagtaat ggattccggc ccaggcggac
      301 gtgtcgtcga gcgaggagcc gaaatcgtcg cgcgtcgcgt actcggcgaa ccagaagagt
      361 cgggcgccgc ggtagccgcc ccgcgtcagc cactcgcgca tcgagatccg gtcgagcgcc
      421 gtccactccg cgtcgtcgga ggagcgccgg cgggggaggg cgaacgcccg gcgccccttc
      481 gcgtcgcgga gccccgcgaa gcggtgcatc tcccgctcga atcgggcgag ctcctcgcgg
      541 tcctcccggg aggcgccggc gcgcgggtag agcccctcgt accagaggcc gcggaagtag
      601 aggcgctcct ccggctcggc gcagagcatc tcctcggccc actcgggggc gccgtccgcg
      661 ctgcggccgg cgacggcgcc gatctcctcg aggagcgtct cgagcgccgc gttgccgtac
      721 atcggcaccg ggacgtagtg cgcgccccac gggtacgcag agacggcgtt cgcgcccgat
      781 cgggcgttcc cgcccggctc gtcctcgagc tcgtagatcc ggaagtcggt gaagccggcg
      841 cggcggaacg tccacgcggc cgagaggccg gagatcccgg cgccgaggat cgcgaccccg
      901 acgctctcct tccgggcgac ggggcgcgag aggagctcgc cggtccggat ccggtggccg
      961 cgcaggacgt cggcccccac gatcgcgccg gagaaggccg gggcgtccgg gccgccgcgg
     1021 caggccgcga ggagggacga gagggccggc aggccggcga gcgcggcgat cgcctcgcgg
     1081 cgggagggcc tcacgtcact cgaacgcccg ccactcgctc tcgtaggtcc ggacgaggac
     1141 ctggtcgtgg aggcggttga cgggcgcctc ggcccacggc gccatgtcgg gcccgaacga
     1201 gaagagggcg ggaagcgtct cgcgcgagag gaagcggagg ccggggagga cgtcggacgg
     1261 gacgtcgaac gggcggggcg aggcgaggac gaaaccccac tcgccgaagg acgggacggc
     1321 ggcgtggtag gcacgcgtct cgaagccggt cgcggcgatc gtccggtcga tgatccagaa
     1381 cgagcgccgg gcgaagagcg gagaggtcga ctggacggcg aaggcgccgt ccggcgcgag
     1441 gcgccgcttc aggagcccgt agaagcgctt cgtgtagagc ttcccgaccg cgaagctgtt
     1501 cgggtcgggg aaatcgacga agacgaggtc gaagaggccg gtctccgtct cgagccactt
     1561 catcgcgtcg tcgttgacga ccgagacctt cggcgagagg agcgagccgc cgttcagctc
     1621 gcggaagagc gggttctcgc gggcgagacg ggtcacgagc gggtcgaggt cgacgaggac
     1681 gacctcctcg acggacgggt gccggagcac ctcgcggacg gcgaggccgt ccccgccgcc
     1741 caggaccgcg attcgccggg cctcggggac gacggcgaag gcggggtgga cgagcgcctc
     1801 gtggtagcgg tactcgtcga cggaggagaa ctgcaggtgg ccgttcagga agagctggaa
     1861 cccggcccgg ttgcgggtca ggacgacccg ctggtagggg gagctcttcg cgaggaggac
     1921 ctcgtcggcg tactgctgct cctcggccag gccggagagc cggtcggcgg cgacgaagcc
     1981 tccgaggagg agcgccgcga cgaggcccgc cttcgcgtag aggccgccgg cgggaccgat
     2041 ctcgtcgcgg aacagccgga tcgacgtgaa ggcgacggcg acgttcagga ggccgaagag
     2101 gagcgacgtg cggaagaggc cgagcgtcgg gacgaagacg agcgggaaga ggagggaggc
     2161 ggcgagcgcc ccgaggtagt cgaacgtcag gacctgggcg acgagctcct tgaacgccac
     2221 cgagcgccgg aggagccgga gcatcagcgg gatctcgacg ccgacgagcg tcccgacggc
     2281 gaagacgagg ccgtagaaga ccgggtggaa ccagcgcggc gagaggaacg cgaacgagag
     2341 gatgagggcc gagaagccgc cgaggaacgc cacggcgatc tcgatctcca cgaaccggcg
     2401 cgcgacgccc ttctcgatga acttcgagag ccaggcgccg gcccccatcg ccgtcaggta
     2461 gatcccgatg acgagcgaga actgggtgac ggtgtcgccg aggaggtagc tcgcgagcgc
     2521 cccggcgacg agctcgtaga cgagcccgca cgtggcgatg acgaggacgg tcaggtagac
     2581 cggccccggc cggaccgggc tcggcgtcac cgggtcacga gccgatggcg gacccgatga
     2641 tgaacgccat cccgaggacg accgccgcga tcacgatccc gagcgcgacg ttctggtcct
     2701 cctcgatctc cttcttcagg gagaagggga ggatgcgcgg caggacgagg aagaagagcc
     2761 cgaagaccgc gatcccgatc gccgagaaga cgagcgccga ccagacctgt cccgcgtgga
     2821 gctgccacca gttcatcact tccctcccgt ccagaagctg taggcccgga atccggtcgc
     2881 gctccgcgcc ccggcggcga cgacaccctt cttcgcgttc gtcggaagct cccacccggc
     2941 gaggccggcc caggcgtagg ccgtcaggag gagcgcaccg aagacctgga acccgcgttt
     3001 cagcatgtcg gcctcactcg tcgtcgtccg agccgctccc cccgccgtag tcgctctcgg
     3061 cccaccgccg tccctcgaac cgggccttgg agatcgcgag cgcgatcggg ccgagcagga
     3121 ggaggaggag gacggccatc aggtgagcga accgggggac gccgctccga aggcggatgt
     3181 cgtagtaggg gggcgctttc ccgcgctcga gctccggctc gaggcggagg acgtagcgcc
     3241 cggcggggac gcgcccgacg tagacggtcc gcgagcggga gccttcgctc cacctctcgc
     3301 cgccgtcgac gccgtggtag tagtcggact ggaggccgaa gccgtacgcc tgcccggtcg
     3361 cctcttcgag gaggaccgct ccgacgtaga cccaggagtt gtcggtcggc gcgtcgatcc
     3421 gcgcctcgac gttcccggcg tgcggcacct cgaacggctc cgtgacgacg gtgagcgcct
     3481 cggcgacggc gtcgggggcg atcgtcgggt cggtggcggc ggtggagagg tcgtgccgct
     3541 tctggtaaac gaccttccgg tcggccttga tgttgaagaa gacgaagagg aagacggcga
     3601 tcccggcgaa gagggcgacg gtgcggagga agccgcgggc cgtctcggcg tgcggccagg
     3661 gctggttcga tccgacccct tcgggcgccg gaaggggcgc cccgaggcgg aagcccgact
     3721 cgagctcctc cttcgggacg tacgacccct ccgaccaggt cacctcgttc gcgtaccgct
     3781 cctccgagag gatgcggggc ggcgcgacgt agtcggagac gtcggtcgac tcgcccttct
     3841 tcacctccca gtagaactcg ccgaggacgg cctcgacgcg ggcggtcgtc gactggaagt
     3901 gcctgtagcg cgtccctcgc cagaccgcga gccgcggcgc ggtcgagacc tcgcccgcgg
     3961 ccgccggctc gacgagcgtc cagtgtccgt tcgactcgac gagccagtgg taggcctcgc
     4021 tcttcgtctc cttgaggagg tactcccgcc agaagtagtg gacgccttcg acggtgcagg
     4081 ccttgaggac cgcgccgagg atcgcgtagg gccgtccgcg aagcgttccc tcgccgccga
     4141 gcgggaggag cggcttgaac ggcagcttct ggagcctcgc gaggacctcg aacttctccg
     4201 ccgaggcccc ctcccggggg gtggcgagga cggagccgca gtaggtgcag gcgacgcgga
     4261 cggtgttcgc cgggtccttc agctggaggc ccgcgccgca ggcggggcag ttcagcgagg
     4321 aggcgcgggc ggcgaccttc cgctcgctcc acccctcgag cccttcgacg ccgaggtcgg
     4381 cgagcgcgac ggagcgcccg acgaagaagg cgtcgacgcc ggggtcgtcg ccgtagtcga
     4441 gcgtcccgac gctcccgtcg gcgcccgaga ggtcggcgta catgaagacg gcccccggcg
     4501 tcgccgcgaa cgggaggtcg ccctccgccg aggcgtagag cgcctggcgg cggtcggtca
     4561 ccgtgaactc gccgtagccg ccgaaggaga ggcgctggcc gggcgcgatc tggggaaagt
     4621 cgggaggggg cgggttcgtc ggcgggggga gcggccgcgt gacccagaac gtcccctgcg
     4681 cctcggcgag ccagccgtag cggccgtcct cgaacgagac gtgccactcg tcccaggtcc
     4741 cctcgcccgt cgagagctgg agccggccga cgatccggaa cggcttcagc cccgcggcct
     4801 tcggccttcc cgtcatcccg acgcggaacg gcgacgaggt cgggacgagc tcggcgacga
     4861 cgccgagggc ctcgaggttc gcccccttcc gggacactgc ggagcggcac tgcgggcaga
     4921 cggtgacgac cgacgacccg gcgcggaaca ggacctctcc accgcaggac ggacagctgg
     4981 cggcgcgcga gctcacgtgg cggaaggata gaacaggccg gccgcgcccg gggcctcccg
     5041 ccgccccgat tcgtcgctcc ggcgtgccgg ggcgacgcct agacggggag cttcacgcga
     5101 aaccgggccc cgcgcccggg cgggctctcc agggagactt ccccgcccat cgcctccgtg
     5161 agcgtcttga cgatcgacag gccgagcccg gtcgacgact cgccgcccgt cggccgcgcc
     5221 gagaggcgcg cgaacttcgc gaagaggcgg gcgcggtcgg cgtcgctcac cccgggcccc
     5281 tcgtcctcga cgtcgacgag gcccgcgccg ccgtcggcgt ggacggagac gaccacggtg
     5341 gagcgccacg gggagaactt gacggcgttc gagacgacgt tctcgatcac ctgctggatc
     5401 gctccggggt cgccggtcac gacgaccggc gacccgtcga ggcggatccc gatctccttc
     5461 cgcgcggccg gctcctgctg cgtctcgacg acccgccggg agatggcgag gaggtcgcac
     5521 ggccgcctct cgagcgcgac gcggcccgcc tcggcggcgc tcacctcgag gagcccggcg
     5581 acgaggtcgc ggacgcgctc ggccgagacg acgatcttgg ccgccatgtc ggcggcctcc
     5641 ctcctcggga actcctcgtc ccggagcatc tcggcgtagc cgaggatgac gccgaggggg
     5701 ttgcgaaggt cgtgcgcggc gatcccgagg aacgtgctct tctcctcgtt catccgcttc
     5761 aggtcggcgg cgacgctccg caccttctcg ctcgcggcct cgaggagcct catcgtgacg
     5821 aaggtccgcc gctgccacgc ctcccggaag tagccggcga ccgagaagaa gaaccagagg
     5881 agcaggagcg tcccgaggga gacgacgacg acgtcggccg gaaggggcgg cccccagccg
     5941 gggagcttgc ggacgagggc gaccgccggg aaggcgagga cgatgaggcc ggccctcacg
     6001 acgctcgcgg cgaagcccga ggggaggagg ccgatcgcgg cggaggcgag cgggaagctg
     6061 agggcggcga agtagacggt cgtccccggc tcctgcggct gcccgacggc gatcatcgcc
     6121 gcgacggccc agcctgcggc gacgctcccc acctcgagga gcggccgcgt ccagcgggcg
     6181 aagcgtggga gagacgcgag gaaggcgagg accgcgacgg gcagcgccag cgcgacgagg
     6241 cggatgacga ggacgctccg ccaggtctcg gggaacgcga tcgggtcgag gaccccgaac
     6301 gcgaggatcg tcgcgagcgc gacgaagacc gaagggcgga ggatgcgggc gctcgaggag
     6361 cgctgctcct cgcggaaggc cgcctcgacg agagggtcgc ggaaccggag cttccacgcg
     6421 tcgtcgaggt cccgttcgct caccgggacg acgggtccct cccgcttcgg cgggcgcggg
     6481 atgcgccatc ccgaggagga gtgtcgtccc ccgagcaccg tgccgctggc catcgatgcg
     6541 gattctgcac cggaccccgc cccttcgcct cgcacttttc ggccggggtc acccccggag
     6601 aaggtcgaag agcttccagg tgaggccggc cacgagcgcg gaagcgggga tcgtcaggac
     6661 ccaggcgacg acgatgctcc tcgcgacgcc ccaacggacc gaggagaggc cgcgggtcgc
     6721 gccgacgccc atgatcgccc ccgtgatcgt gtgggtcgtc gagaccggga tgccgccgag
     6781 cgaggccccg acgagcgtca cggccccggc gagctcggcg caggagccgc cgatcggctt
     6841 caacggggtg atccgcatcg acatcgtccg gacgatgcgc cagccgccgg agagcgtgcc
     6901 gaggccgatc gcgctgaagc agagcaggac gatccagaac ggaatgtggt tcccggtgag
     6961 gtgcccggtc gagagaagga gcgcgaagat gatccccatc gtcttctgcg cgtcgttcat
     7021 cccgtggccg aacgagaatg ccgcggcgga gaggagctgg accttccgga agacccggtc
     7081 gacccggtcg gggcgccagc gccggaaagc ccactgggca ccgaggaagt tcaggaagcc
     7141 gagcgcgagg ccgaggaggg gcgagaggac gatgaaggcg ctcgtgacga ggagcccctt
     7201 cagaacgagg ctcccggccc cggccttcac gaacgccgcg ccggcgaagc cgccgatgag
     7261 cgcgtgcgag gaggaggacg ggaggcccca ccaccaggtg aggaggttcc agacgatcgc
     7321 gccgatgagg ctcgcgagga cgaggtcggg gtcgacgacg tcggagcgga cgatcccctt
     7381 cccgatcgtc tgcgccacgc gcgtctcgag cgcgaaggcc gcgacgaagt tgaagaaggc
     7441 cgcccagacg accgcgaccc ggggcgagag gacgcgcgtc ccgacgaccg tggcgatgga
     7501 gttcgccgcg tcgtggaagc cgttgatgta gtcgaagagg agtgcgacga cgacgatgag
     7561 gacgatgtac gtcacggcgg ctcaggtcga cttgatgacg atcgcctcga gcgtgttcgc
     7621 gacgtcctcg cagcggtcgg tcgcgccctc gacgaagtcg tagatctcct tccacttcat
     7681 gacctcgatc gggtccctcg cctccgcgaa gagcctcccg atcgcgagct gcgccacggt
     7741 gtcggcctcg ttctcgagcc ggttgatctc gacgcaggcc tcgaggatct gcttcgagtg
     7801 cttgaggtcg gagaggaggg ccatcgcctt ctggatcgcc tccgccgact ggacgatgac
     7861 cgaggtgatc ttcttcagct ccggggtcgt cgaggcgatc cggtagagga cgatccgcgc
     7921 ggccgcggcg tcgatgaagt cgaggacgtc gtccaggttc cggaccagga cgtggatgtc
     7981 ctcgcggtcg atcggggtga tccaggtccg gttcaggagg tcgaacgtct cgtgggcgat
     8041 ctggtccccc tggtgctcga gctgcttgat ccggaccgcg cgctcgtcgt cgaggaccgg
     8101 cgcggcggcg agggcgtcga ggacgcgcgc ggcctcgagg atcacctcgg cgccgcgggc
     8161 gaactgcgga aggaaggtct cttcacgggg gaacagacgc atcgagggct ctcctccgga
     8221 cggaggaggt tatcagccgc cgcgcggccg cgacgccgcg cggcccggcg gctcagctcg
     8281 gtcgggggga cgccgcttcg cggaggatcg cgacggccgc ggggcggccg gccgccccct
     8341 cgccggagac gcggatcgcc tccgcgcgga gctcgaggtc gccggatccg gtcgcgagcc
     8401 gaagcggtcc ggccgcgcgg ccgccgtcga gggcctcgcg gaggaggtcg gtcacggcct
     8461 tctcgcggac cagctccagg acgtgctcgc ctctccgcag ggcgccggcc gggaggcgga
     8521 ggaggccgag cgccgcggag ttcgcatcga ggagggagag ctcgcggtcg acgacgagca
     8581 gtccggtcgg gattccctcg aagatcgccc gcgaaaccgt ctcctgccgg aggaccgcct
     8641 cgttccgctc gctcgtctgc tccaggaggt cggcgagcgc cgagtggacc gcggccgtct
     8701 cctccaagac ggcgcccggg ttcgccggcc gccggccccg tgcaacggcc tccacggcgc
     8761 ggagcacgtg gccgagctcg gcggcctgcc ggaggcgcag ccggacgaag agggctccgg
     8821 cgagcagccc ggccgcgaca cagaccagga ggacccgccg gcggtagggg gcctcccagg
     8881 ccgtgaggtc cttctcgggg aaggccaggc ggaggacctc tccgctccgg agccgtgcgg
     8941 ccgcgtagac gagccgctgg ccgagcgtcg aggagaagcg ggcctcgacg cctcgccccg
     9001 agcggagcgc gtcgcgcacc tcgggacgtc ccgcgtggtt ctcgagggcg tcgagccggc
     9061 tgcggtcgac gccgctgtcc ccgaggaccc ggccgtcggt cgcgacgagc gtggcccgga
     9121 gggcgagctg ccgcccgatc cggtcgacgg tctccttcgg gtccccgccg gtggcgagga
     9181 ccgcttcgac gtcgggaccg accctctcga gcagcgagaa cgcggtctcc ttcgtccggc
     9241 ccgcgaccgc cccatccagg atccgtccga ggatggcgcc cgcgacgaga ccaccggcga
     9301 gccccaggag cagggggagg acgagccttc gggccaggac cggcgcgcgg agaggggggc
     9361 ggggcgggtc gggcgtcgtc atgagagccc ggaatcgtag cgccgccgcg ttaagaccgt
     9421 gtggggtcgc tcgggggcgg gacgggtgcg gtcgaccgcg agatccgcga ggagggcgcc
     9481 tcgtcccggt agtagccgcg cggggcgagg agccgccagg tccgctcgac gagctccttg
     9541 tccgagacgg gcctggagag gatctcgctc gcgccgacct gccgggcgaa gtgctcctgg
     9601 gcccggtcca cccgcgtccc gaggaggagg accgggacct gggcgaacgt cggcgtcgcc
     9661 ttgaggatcc gcgccacctc gaatccgtcg gccttcggga gggcgatgtc catcacgacc
     9721 gcgtccggaa cccgcgacat caggagctcg aggatctcct cgccgctgcg ggcctccacg
     9781 gcgtcgcagc ccgaatcggc gaaggccttg ccggccgcga tccggtccgc cccgtccccc
     9841 atgacgacga agaccgtcgg ccggccagcg gggcgccgca ccgccggaac ggaggggggc
     9901 gtcgcgaccc cggaggggga acgcatcgaa ggcgaaggcg tcagaatccg ggccggagcg
     9961 ggctcggcct cgggggccgg acgcggacgc ggacgatggg gcacgacggg cgtcgggtcg
    10021 tcgcggaggg cgtcgacggg tcgctgaggc cgggggccgc cgcccgggtc gcccagcgcc
    10081 gcgtcgatcg cccgcgccag cgccgctgcc tcgtcgtcga cctccggaga gtccggctgg
    10141 cccgccgcgc gcgcgagcgc cgcgtcgagc gcggcggcgg tcggcgggcg gtcctcgcgc
    10201 cgcttcgcga gcgcgcggtg gacgacctcc cggaggcgct gcgggatctc ggggtagagc
    10261 gagaggttcc ccggcaccgg ctcggagatc tgcttcatcg cgatcccgag agcggaatcc
    10321 gccgtgaagg ggaggacgcc ggagagcatc tcgaacgcga tgatcccgat cgagtagatg
    10381 tcgctcctca cgtcggcgga ctggcccatc gcctgctcgg gcgagatgta gtgcggcgtc
    10441 ccgaggacga ccgcctcctg cgtcagcttc tcgtcgaccg cgccccgggc gaggccgaag
    10501 tcgaggatcg agacgacgtc gccggctccg acgatgatgt tctggctctt caggtcgcgg
    10561 tggacgatct cctgctcgtg ggcggcctgg gtcccctggc agatctgccg gaggatccgg
    10621 attgcccgtt ccggggggat ccggccttcc tgcaggatga gggactgcag gtcctggccg
    10681 gggacgaact ccatcgtgat gtaggggtgt ccgccgccgc tcccgaagtc gtacatccgg
    10741 gcgacgttcg ggtgcttgac ccggcggttc aggctgatct cgcgcttgaa gcgggcgagg
    10801 atcgccccgt cgtcgtcccc gacgtgcccg tagaggacct tgatcgcgac gacctcgtcg
    10861 aggtcccggt ccctcgcctt gaagacgacc cccatcccgc cgcggccgag gtcctcgagc
    10921 cacgtgtagc gcggggctcc ggcgaagaac tgggttcgaa acgtgtcgag gttcatgtcg
    10981 ggtcctggcc cgcgggcgag cgggtcgtcc cgcgccgaac ccccattatc gcccgcctgc
    11041 gcgattcccg cagtcctacc ggcaccctgt ccccccggcg cacggggcga taggatgcct
    11101 cccgtcccgc aacgacaccc gaggagaccc gcacatgacc acgatcgaca ccatcaccac
    11161 agccctcggc gccgaggcga aatcccttct cgagcacacc tgctccggga tcccgaagga
    11221 gactctccac ctcccgggtc ccgacgtcgt ggaccgcatc tggtcgatgt ccgaccgccc
    11281 gatccccgtc ctccggagcc tccagcagct cgtcgaccac ggccgcctgg ccgggaccgg
    11341 ctacctctcg atcct
//
LOCUS       DAOTEZ010000095        11317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4036_length_11317_cov_6.308471, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000095 DAOTEZ010000000
VERSION     DAOTEZ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11317
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4036_length_11317_cov_6.308471"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="P5164_06880"
     CDS             complement(<1..1167)
                     /locus_tag="P5164_06880"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRY43657.1"
                     /translation="MSSLKALPGLVLLVAGLPALAGARVPPVASYRIEATWDEERKAL
                     AGRETITFVNRTGRPFDDVVLHLYLNGFRNTRSTLFRALPPPSGSDGFGYCELKRIAP
                     SGGEDLTRRATFASGAAGNPDDRTLVRVPLPRPVAPGETLVLEVDFESRLPRGLLRTG
                     WKDDFVLAGQWFPKLAKATEAGWRGRPFHPFTEFFADFGDYDVTLLLPAEVKGRVAVT
                     GRVVEESGGDAGRVRVRARAEDVHDFAFAFSPRFEVKSEKVAPKGLPAVEVRLYLQPD
                     HRRSRERYLRAAREGLALYGSWLGPYPYPILTIVDPPTGSGAEAMEYPTLLTGGATWL
                     SPAAGGEPGAVVLHELAHQWFQGVVATDEVSEAHLDEGLATWIAARAVTRLQGPP"
     gene            complement(1178..1585)
                     /locus_tag="P5164_06885"
     CDS             complement(1178..1585)
                     /locus_tag="P5164_06885"
                     /inference="COORDINATES: protein motif:HMM:NF016211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HRY43658.1"
                     /translation="MKRKLLLAGGMVALGLGLAGLVLPLLPTTPFLLLAAWLFARSSP
                     ERYERLLGHERLGPHVRDWVERHGLRLRVKVRAILLLWAALLPSSLWLGHRHLSGFLL
                     LAVGAAVTAYLLRLPTLEEPPGTAALPKRGDAT"
     gene            1666..3693
                     /gene="ligA"
                     /locus_tag="P5164_06890"
     CDS             1666..3693
                     /gene="ligA"
                     /locus_tag="P5164_06890"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRY43659.1"
                     /translation="MLKLPETAAAAAERAASLRAELRRHEHLYYALARPEVSDAEYDA
                     LSRELLAIEERWPELVTPDSPTRRVGGAPVDGLPNVRHEVPLLSLENAYSEAELDAWL
                     TRVTDRLDGRLPEVVCELKIDGLSISLVYEEGVLVRGATRGDGTTGEDVTPNVRTIRV
                     LPLRLPEGAPRLLEVRGEVYLGKSSFQALNAAREEAGEPLFANPRNAAAGSLRLLDSR
                     ETARRRLSVFLYSVARWEGDRAPATQEEALGALARLGLPVNPHHAVARSGAEVKAFLA
                     EWREKRHTLDLETDGAVLKVSPVSDQQRLGATAKFPRWALAYKFPAEEATTRVTAIVV
                     QVGRTGVLTPVAEFEPVLLAGSTVRRATLHNYEDLSRKDVRVGDTVAVEKAGDVIPKV
                     TRVLLEKRPPEAVPFEMPAACPECGEAVVREEGEVAVRCVSGSCPAQVKEAIRHYVAR
                     RAMDVEGLGEERVDQLLAAGLIADVAGLYALRAEHLAALERWGEKSAANVLAEIERSK
                     EAGLARLLFGLGIRHVGEKTGRLLARRFGAMEALAAATEEELTAVPEIGPETSRSVRD
                     WFGLPANVRLLSKLSEAGVRMNEPEGAIAPGGPLSGMTFVLTGTLPRRTREEATAAIE
                     RAGGKVSGSVSRKTAAVVAGEEAGSKLEKARALGVPVWSEDDLERAIGGGR"
     gene            3690..5147
                     /locus_tag="P5164_06895"
     CDS             3690..5147
                     /locus_tag="P5164_06895"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRY43660.1"
                     /translation="MSAAPPREPLVLVVDDDAGSRRAMALTLTTDGRRVEEFADADSA
                     LSRAMDDPSVGLVVTDLKMPGKTGIELATELAAARPDVSVLLVTAFGDIETLLRAKDL
                     GTVDYVAKPVARDDLRLRASAALGRARQAGEIKQLRERLDKRYGFEAIIGVSAAMERV
                     FDVLRKVAPTRMNVLITGESGTGKELVANALHHNSPRRPRAFVALNCGAIPKEIIESE
                     LFGHEKGAFTGALVKRMGRIEQAHGGTLFLDEVSEMPPDLQVKFLRVLEERRVTPVGG
                     NDSKEVDFRLVSATNRDLKKEIEAGRFRQDLYYRLKGVVVDLPALRERREDVPLLAER
                     FREVFAKEHERAVTGFTPAALSALMSYDWPGNVREMKSAIEWAVFSAAGPRIDVSDLP
                     PDVKGDADGEGPHPEGTVPVAHSAILVGKTMAEIEKEAILTALQASGGNRRKAAERLE
                     IGLRTLQRKLKEYRGETGGDEGEDEADDDGSEPSA"
     gene            5183..5398
                     /locus_tag="P5164_06900"
     CDS             5183..5398
                     /locus_tag="P5164_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43661.1"
                     /translation="MDIPAKVYITCPSAELKKQPGTLIAVSANGYYEVHVQFGSNMHA
                     MLLPITETALLNAEPVLTPPAGFELER"
     gene            complement(5575..7890)
                     /gene="ppk1"
                     /locus_tag="P5164_06905"
     CDS             complement(5575..7890)
                     /gene="ppk1"
                     /locus_tag="P5164_06905"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017286675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRY43662.1"
                     /translation="MIRHEQRPRRKRSREAIEEPLPAPFEPPDEPAPAELPAGPPGDV
                     EERAEGGPSVPLLNRELSWLDFNQRVLEEARDERWPLLERLKFLCISESNLDEFFMIR
                     VSGLRDQLSADLAELSDDGLTAREQLALVRKGVLAMEAEQTACLHEDLLPRLAAAGIT
                     LLVWGDLDEERREAARSYFRRNVLPVLTPLAVDPGHPFPFLSNLSVNLAIETRNPENG
                     EVKFARVKMPPAIPRLLPLRTVVEGKKKVKATHAEFLLLEELVQANLDELFPGLEILA
                     SYLFRITRDADIEIQEDEASDLLATIEQEVRRRRFGAVVRVEVTPQTPKKMRKLLMRQ
                     LEVSESDIYEVVGILGAADLMALVKLDRRDLKDAPFAPALPPALAAPEGSIFQAIRQG
                     DLLLHHPYDSFGPVVELIEEAADDPKTLAIKMTLYRTSSDSPVIPALVRAAEAGKQVA
                     VLVELKARFDEENNIVWAKALERAGIHVVYGVVGLKTHAKIALVVRREKDEIRRYVHL
                     GTGNYNPATAKVYTDLGLLTCRPEFGEDATRLFNTLTGLATRTSYNRIVTAPRDLHRT
                     VLDWISRETEHARAGRPARIRAKLNALVDTAVIKALYDASRAGVPVDLIVRGTCCLVP
                     GLPGVSGTVRVRSIVGRFLEHSRFFLFENGGDPEVWASSADWMPRNFFRRVETAFPIV
                     DPELGRQLSDAFDAMMADNARARALRSDGTYERLRPGRGEPAIDSQAFFLDQARRRVL
                     KAAEHFVKGAAADGFDRVPDPRERGPEDDGR"
     gene            complement(7908..9239)
                     /gene="glmM"
                     /locus_tag="P5164_06910"
     CDS             complement(7908..9239)
                     /gene="glmM"
                     /locus_tag="P5164_06910"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRY43663.1"
                     /translation="MRALFGTDGIRGRAGEFPLDPATVSRVGGALASVLREAGRPSPL
                     TVVLGCDTRVSSPGIVAALSGGLIAAGAGVRFAGVVPTPAVAHLVRALGADAGISISA
                     SHNPWPDNGIKVFSSEGRKLPDALEAEIERRIATAAPAEPIPPAEDSALAELYETHLV
                     ETLPARLDGLKVVVDCANGAAFRVAPEAFRRAGAEVVPLAAAPDGRNINEGCGALHPE
                     AMANAVVRTGAHLGVALDGDADRAILADGEGRLLDGDDVLLLWALELEREGKRPGSVV
                     GTVMSNWGLEEALSSRGIRLVRAAVGDRYVVEEMERHSALLGGEQSGHLIRADLTTTG
                     DGTLTGLHVAAVVATTGVPLASGARFTRAPQVLRNVKVREKVPFERVPGFPHLVAGAE
                     NRIAGSGRILVRYSGTELLARVMVEGTERATVESIADELAGALQGALGASD"
     gene            complement(9277..10167)
                     /gene="folP"
                     /locus_tag="P5164_06915"
     CDS             complement(9277..10167)
                     /gene="folP"
                     /locus_tag="P5164_06915"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRY43664.1"
                     /translation="MSARELRLGRRTIRFGPSASVMAIVNATPDSFSDGGRFPAAGDA
                     VRAGLGYARAGAVVVDVGGESTRPRGAAYGTGAGEVSPAEETARVVPVVAGIRAGDPE
                     VPISIDTRRTAVAREALAAGADVVNLVAGLDPPGDLLELVAERDAAIVLGHMRGTPAT
                     TFEVSRFGPDVVSDVAHDLARARRRCLVAGIRPDQILLDPGLGFGKSAEQNFQLLARL
                     EEIGPDGVPIVLGASRKAFLGSVSGRPAAERLPESLAACAVAIERLRDRNPVLLRVHD
                     VDETLRFVHVLDAAAAASRR"
     gene            complement(10187..>11317)
                     /locus_tag="P5164_06920"
     CDS             complement(10187..>11317)
                     /locus_tag="P5164_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY43665.1"
                     /translation="GLGGGHDEREQTLNALLVEMDGFEGNDGVILIAATNRPDVLDPA
                     LLRPGRFDRRVVVDRPDVKGREGILKVHVKNIPMSDDVDLSVVARGTPGFAGADLANL
                     VNEAALTAARADKKKVEMDDFNYAKDKVLMGAERKSMVMSDKEKRNTAYHEGGHALIA
                     AFEEHADPLHKVTIIPRGRALGVTQQLPLEDKYSYNKEYVEARLAVMMGGRLAEEVCL
                     GQVTTGASNDFEQATTMARRMVCEWGMSDLGPMTFGHEEGEVFLGKDFARRADYSEDT
                     ARKIDAEVNRIISSAYLRGKQIVLDNRPALDAIAAELLEKESLDGEEVYVLIEKVSGK
                     RVPHTMKSHPEDAARGAASTVSAFGATPAVSDGAPPADPVPA"
BASE COUNT         1598 a         3923 c         4281 g         1515 t
ORIGIN      
        1 gggcggtccc tggagccgcg tgacggcgcg ggcggcgatc caggtcgcga gcccctcgtc
       61 caggtgcgcc tcgctcacct cgtcggtcgc gacgacgccc tggaaccact ggtgcgcgag
      121 ctcgtgaagg acgacggctc ccggctcgcc gccggcggcc ggggagagcc acgtcgctcc
      181 gccggtcagg agcgtcgggt actccatcgc ctcggccccc gagccggtcg gtgggtcgac
      241 gatcgtcagg atgggatacg gatacggccc gagccagctg ccgtagagcg cgagcccttc
      301 ccgggcggcg cgcaggtagc gctcgcgcga gcggcggtgg tcgggctgaa ggtagagccg
      361 gacctccacc gcggggagcc ccttcggcgc gaccttctcc gacttcacct cgaatcgggg
      421 cgaaaacgcg aaggcgaagt cgtggacgtc ctccgcgcgg gccctcacgc ggacgcgccc
      481 cgcgtccccg ccggactctt cgacgacgcg cccggtgacg gcgacgcgcc ccttcacctc
      541 ggcgggcagg aggagcgtca cgtcgtagtc tccgaagtcg gcgaagaatt ccgtgaacgg
      601 gtggaacggc ctcccgcgcc agcccgcctc cgtcgccttc gcgagcttcg ggaaccactg
      661 gccggcgagg acgaagtcgt ccttccaccc cgtccgaagg aggccccgcg ggaggcggga
      721 ctcgaagtcg acctcgagga cgagcgtctc tccgggcgcg acgggacgcg ggagggggac
      781 gcgaacgagc gtccggtcgt cggggttgcc ggcggcgccc gacgcgaacg tggcccggcg
      841 cgtcaggtcc tcgccgccgg aaggggcgat ccgcttcagc tcgcagtagc cgaatccgtc
      901 ggagcccgat ggtggcggga gcgcgcggaa gagcgtcgat cgggtgttgc ggaagccgtt
      961 caggtagagg tggaggacga cgtcgtcgaa gggccgtccg gttcgattca cgaacgtgat
     1021 cgtctcgcgg cccgcgagcg ccttccgctc ctcgtcccag gtcgcctcga tgcgatagga
     1081 ggcgacggga ggcacgcgcg ctccggcgag agcgggcagg ccggcaacga ggagaacgag
     1141 ccccgggagc gccttcagcg aggacacggc cgcgatgcta ggtggcgtcg cccctcttgg
     1201 gaagggccgc ggttcccggc ggctcctcga gcgtcggaag gcggaggagg tacgcggtca
     1261 cggccgcgcc gaccgcgagg aggaggaatc ccgagaggtg ccggtgcccg agccagaggg
     1321 agctcggcag aagggccgcc cagagcagga ggatcgcccg gaccttcacg cggaggcgca
     1381 ggccgtgccg ttcgacccag tcgcgcacgt gggggccgag ccgctcgtgc ccgaggaggc
     1441 gctcgtaccg ctcgggcgag ctgcgggcga agagccaggc cgcgaggagc aggaacgggg
     1501 tcgtcgggag gagggggagc acgagacccg cgagcccgag gccgagcgcg accatcccgc
     1561 ctgcgaggag gagcttccgc ttcatccgtc cgtcgccgcc ggcccgcccg gcggacgcgg
     1621 gagtatcgcc cgccggccgg cgggacgacc cctacaattc cgagcgtgtt gaagctcccc
     1681 gagacggccg cggcggccgc ggagcgtgcg gcgtccctgc gcgccgagct ccgccggcac
     1741 gagcacctct actatgccct cgcccgcccc gaggtcagcg acgccgagta cgacgcgctc
     1801 tcgcgcgagc tcctcgcgat cgaggagcgc tggcccgagc tcgtcacgcc cgactcgccg
     1861 acgcgccgcg tgggcggggc cccggtcgac ggcctcccga acgtccgcca cgaggtgccg
     1921 ctcctctcgc tcgagaacgc ctactccgag gcggagctcg acgcgtggct gacgcgcgtg
     1981 accgaccggc tcgacggacg gctcccggag gtcgtctgcg agctgaagat cgacggtctc
     2041 tcgatctccc tcgtctacga ggagggggtc ctcgtccgcg gagccacgcg cggcgacggg
     2101 acgaccggcg aggacgtgac gccgaacgtc cggacgatcc gcgtcctccc gctccgcctt
     2161 cccgagggcg ccccgcgcct cctcgaggtc cggggcgagg tctacctcgg caagtcctcc
     2221 ttccaggcgc tgaacgccgc ccgcgaggag gcgggcgagc ccctcttcgc gaacccgcgg
     2281 aacgcggcgg ccggctcgct ccggctcctc gactcgcgcg agacggcgcg gcggcggctc
     2341 tcggtcttcc tctactccgt ggcgcggtgg gaaggggacc gggcgccggc gacgcaggag
     2401 gaagcgctcg gggcgctcgc ccgcctcggc ctccccgtga acccgcacca cgccgtcgcg
     2461 cgaagcgggg ccgaggtgaa ggccttcctc gccgagtggc gcgagaagcg gcacacgctc
     2521 gacctcgaga ccgacggcgc ggtcctgaag gtgagccccg tctccgacca gcagcgcctc
     2581 ggagcgaccg cgaagtttcc acgctgggcg ctcgcctaca agttcccggc cgaggaggcg
     2641 acgacgcgcg tgaccgccat cgtcgtccag gtcgggcgga ccggtgtcct gacccccgtc
     2701 gccgagttcg agccggtcct cctcgcgggg tcgacggtcc gccgcgcgac gctccacaac
     2761 tacgaggacc tctcccggaa ggacgttcgg gtcggcgaca cggtcgccgt cgagaaggcg
     2821 ggggacgtca tcccgaaggt gacgcgcgtc ctcctcgaga agcgccctcc cgaagccgtt
     2881 ccgttcgaga tgcccgcggc ctgccccgag tgcggcgagg cggtcgtccg ggaggagggg
     2941 gaggtcgcgg tccggtgcgt cagcgggtct tgccccgcgc aggtgaagga ggcgatccgc
     3001 cactacgtcg cccgccgggc gatggacgtc gaagggctcg gtgaagagag ggtggaccag
     3061 cttctcgcgg ccggactcat cgccgacgtc gccgggctct acgccctccg cgcggagcac
     3121 ctcgcggcgc tcgagcgctg gggcgagaag tcggccgcga acgtccttgc ggagatcgag
     3181 cgatcgaagg aagcggggct cgcgcggctc ctcttcggcc tcgggatccg ccacgtcgga
     3241 gagaagaccg gcaggctcct ggcgcggcgg ttcggggcga tggaggcgct cgcggcggcg
     3301 accgaggaag agctgacggc cgttccggag atcggaccgg agacgtcgcg ctccgttcgg
     3361 gactggttcg ggcttccggc gaacgtccgc ctcctctcta aactctcgga ggccggcgtc
     3421 cgtatgaacg agccggaggg ggcgatcgcc cccggagggc cgctgtccgg catgaccttc
     3481 gtcctgacgg ggacgcttcc gcggcggacg cgggaagagg cgacggcggc gatcgagaga
     3541 gccggaggca aagtgagcgg aagcgtttcg aggaagacgg ccgcggtcgt ggccggcgag
     3601 gaggccgggt cgaagctcga gaaggctcgc gcgctcggtg tgccggtctg gtccgaggac
     3661 gacctggagc gagcgatagg gggcgggcga tgagcgcggc ccctccgcgc gagccgctcg
     3721 tcctcgtcgt cgacgacgac gcggggagcc ggagggcgat ggccctgacg ctgacgacgg
     3781 acggccgccg cgtggaggag ttcgccgacg ccgactcggc cctctcgcgc gcgatggacg
     3841 acccctccgt cggcctcgtc gtgaccgacc tgaagatgcc ggggaagacc ggcatcgagc
     3901 tcgcgacgga gctcgccgcg gcgcgccccg acgtctccgt cctcctcgtg accgccttcg
     3961 gcgacatcga gacgctgctc cgggcgaagg acctcggcac ggtcgactac gtcgccaagc
     4021 cggtcgcgcg cgacgacctg cgcctccgcg cgtcggccgc cctcggccgc gcgcgccagg
     4081 cgggcgagat caagcagctc cgcgagcggc tcgacaagcg ctacggcttc gaggcgatca
     4141 tcggcgtctc ggcggcgatg gagcgggtct tcgacgtcct ccggaaggtc gcgccgacgc
     4201 ggatgaacgt gctgatcacg ggcgagtcgg ggacgggcaa ggagctcgtc gcgaacgcgc
     4261 tccaccacaa ctcgccgcgc cggccgcggg cgttcgtggc gctcaactgc ggcgcgatcc
     4321 cgaaggagat catcgagtcg gagctcttcg ggcacgagaa gggggccttc accggcgcgc
     4381 tcgtgaagcg gatgggtcgg atcgagcagg cgcacggcgg gacgctcttc ctcgacgagg
     4441 tctcggagat gcctcccgac ctgcaggtga agttcctgcg ggtcctcgag gagcggcggg
     4501 tgacgcccgt cggcgggaac gactcgaagg aggtcgactt ccgcctcgtc tccgcgacga
     4561 accgtgacct gaagaaggag atcgaggcgg ggcggttccg gcaggacctc tactaccggc
     4621 tgaagggggt cgtcgtcgac ctgccggccc tccgcgagcg gcgcgaggac gtcccgctcc
     4681 tcgccgagcg gttccgcgag gtcttcgcga aggagcacga gagggccgtg acgggattca
     4741 cgccggcggc gctctcggcg ctcatgtcgt acgactggcc cgggaacgtc cgcgagatga
     4801 agagcgccat cgagtgggcc gtcttcagcg ccgcgggccc gcggatcgac gtctccgacc
     4861 ttccgcccga cgtgaagggc gacgcggatg gagaaggccc ccaccccgag gggaccgttc
     4921 ccgtcgcgca ctcggcgatc ctcgtcggca agacgatggc cgagatcgag aaggaggcga
     4981 tcctgaccgc tctccaggcc tcgggcggca acaggcgaaa ggcggccgag cgtctcgaga
     5041 tcggccttcg gacgctccag cggaagctga aggagtatcg cggcgagacg gggggcgacg
     5101 agggggagga cgaggccgac gacgacggca gcgagccctc cgcttagaat ccggtcgaac
     5161 ccgacgagcg aggaggagcc gaatggacat cccggcgaag gtctacatca cctgccccag
     5221 cgcggagctg aagaagcagc ccgggacgct catcgccgtt tcggcgaacg gctactacga
     5281 ggttcacgtg cagttcggct cgaacatgca cgcgatgctc ctcccgatca ccgagacggc
     5341 gctcctgaac gccgagccgg tgctcacgcc gccggcgggc ttcgagctcg aaaggtaagc
     5401 cgggggaccc gtccccgccc gggtcccccg gacccccccg ccgcggagag gaagggagcg
     5461 agggaccggt aagccggggg acccgtcccc gcccgggtcc cccggacccc cccgccgcgg
     5521 agaggaaggg agcgagggac cggtaagccg ggggacccgt ccccgtcgtc cgggtcagcg
     5581 gccgtcgtcc tcggggccgc gttctcgcgg gtcggggacg cggtcgaagc cgtccgccgc
     5641 ggcgcccttg acgaagtgct cggcggcctt cagtacgcgg cggcgcgcct ggtcgaggaa
     5701 gaaggcctgc gagtcgatcg cgggctcgcc gcggccgggg cgaagccgct cgtaggtccc
     5761 gtcggaacga agcgcacgcg cccgggcgtt gtcggccatc atcgcgtcga acgcgtcgga
     5821 gagctgccgg ccgagctcgg gatcgacgat cgggaacgcg gtctcgacgc gccggaagaa
     5881 gttccggggc atccagtcgg ccgacgaggc ccagacctcg gggtcgccgc cgttctcgaa
     5941 gaggaagaag cggctgtgct cgaggaacct cccgacgatc gagcggacgc ggaccgtgcc
     6001 cgagacgccg gggagccccg gcacgaggca gcaggtgccg cggacgatca ggtcgaccgg
     6061 gactccggcc cgggaggcgt cgtagagggc cttgatgacg gcggtgtcga cgagcgcgtt
     6121 gagcttcgcc cttatccggg cgggccgccc cgcgcgcgcg tgctccgttt cgcgcgagat
     6181 ccagtccagc acggtccgat ggaggtcgcg cggggcggtg acgatcctgt tgtaggaggt
     6241 ccgcgtcgcg aggccggtga gggtattgaa gaggcgcgtc gcgtcctctc cgaactcggg
     6301 gcggcaggtg agcaggccga ggtcggtgta gaccttcgcc gtcgccgggt tgtagttccc
     6361 ggtcccgagg tggacgtagc ggcggatctc gtccttctcc cgccgcacga cgagggcgat
     6421 cttggcgtgg gtcttcaggc cgacgacgcc gtagacgacg tggatgccgg cgcgctcgag
     6481 cgccttggcc cacacgatgt tgttctcctc gtcgaagcgc gccttcagct cgaccaggac
     6541 ggcgacctgc ttgccggcct ccgccgcccg cacgagggcc gggatgacgg gggagtcgga
     6601 gctcgtgcgg tagagcgtca tcttgatcgc gagcgtcttc gggtcgtcgg cggcctcctc
     6661 gatcagctcc acgaccggcc cgaacgagtc gtaggggtgg tggaggagga ggtccccctg
     6721 tcggatcgcc tggaagatgg aaccctccgg cgcggcgagc gcggggggca gcgccggggc
     6781 gaacggggcg tccttcaggt cgcgccggtc gagcttcacg agcgccatga ggtcggccgc
     6841 gccgaggatc ccgacgacct cgtagatgtc gctctcgctc acctcgagct ggcgcatcag
     6901 gagcttccgc atcttcttcg gcgtctgggg cgtcacctcg acgcggacga cggcgccgaa
     6961 ccggcgcctg cggacctcct gctcgatcgt ggcgaggagg tcggaggcct cgtcctcctg
     7021 gatctcgatg tcggcgtcgc gcgtgatccg gaagaggtac gaggcgagga tctcgaggcc
     7081 cgggaagagc tcgtcgaggt tcgcctggac gagctcctcg agcaggagga actccgcgtg
     7141 cgtcgccttg accttcttct ttccctccac gacggtccgg agcgggagga ggcgcgggat
     7201 cgccgggggc atcttgacgc gcgcgaactt cacctcgccg ttctcggggt tccgggtctc
     7261 gatggcgagg ttcaccgaga ggttcgagag gaacgggaac ggatgcccgg ggtcgacggc
     7321 gagcggcgtc aggacgggga ggacgttcct gcggaagtag ctccgcgccg cctcgcgccg
     7381 ctcctcgtcg aggtcccccc acaccagcag ggtgatcccg gccgccgcca ggcgcgggag
     7441 gaggtcctcg tggaggcagg ccgtctgctc ggcctccatc gcgaggacgc ccttccggac
     7501 gagcgcgagc tgctcccgcg ccgtcaggcc gtcgtcggag agctcggcga ggtctgcgga
     7561 gagctggtcc cggaggcccg agacccggat catgaagaac tcgtcgaggt tgctctcgga
     7621 gatgcagagg aacttgagcc gctcgaggag cggccacctc tcgtcgcgcg cctcctcgag
     7681 cacccgctgg ttgaagtcga gccaggagag ctccctgttg aggagaggca cggacggccc
     7741 gccctcggcc cgctcctcga cgtccccggg cggcccggcc gggagctccg cgggcgcggg
     7801 ctcgtccggc ggctcgaacg gcgccgggag gggctcctcg atcgcctccc ggctccgctt
     7861 tcgccgcggg cgctgctcgt ggcggatcac ggttctctcc ccggcgctca gtccgaggct
     7921 ccgagcgcgc cctgcagcgc tccggcgagc tcgtcggcga tcgactcgac ggtcgccctc
     7981 tccgtgcctt cgaccatgac gcgcgcgagg agctccgtcc cggagtagcg gacgaggatc
     8041 cgtcccgatc cggcgatccg gttctcggcc ccggcgacga ggtgcgggaa gcccgggacc
     8101 cgctcgaacg ggaccttctc cctcaccttc acgttgcgca gcacctgggg agcgcgcgtg
     8161 aatcgggcgc cggacgcgag ggggaccccg gtggtcgcga cgacggcggc gacgtggagg
     8221 cccgtcagcg tcccgtcccc cgtcgtcgtc aggtcggcgc ggatcaggtg ccccgactgc
     8281 tcgcccccga ggagagcgga gtgccgctcc atctcctcga cgacgtagcg gtcccccacg
     8341 gccgcccgga cgagccggat gccgcgcgag gagagggcct cctcgaggcc ccagttcgac
     8401 atgacggtcc cgacgaccga gcccggcctc ttcccctccc gctcgagctc gagcgcccag
     8461 aggaggagga cgtcgtcccc gtcgaggagg cgtccctcgc cgtcggcgag gatcgcccgg
     8521 tcggcgtcgc cgtcgagcgc gacaccgagg tgcgcgccgg tgcggaccac ggcgttcgcc
     8581 atcgcctccg ggtggagagc gccgcaccct tcgttgatgt tccggccgtc cggtgcggcc
     8641 gcgagcggca cgacctcggc cccggcgcgc cggaacgcct cgggggcgac gcggaaagcg
     8701 gctccgttcg cgcagtcgac gacgaccttg agcccgtcca gtcgggccgg aagcgtctcg
     8761 acgagatggg tctcgtagag ctccgcaagc gcggagtcct cggctggcgg gatcggctcg
     8821 gccggcgcgg cggtggcgat ccgccgctcg atctcggcct cgagggcgtc ggggagcttc
     8881 cggccctccg aggagaagac cttgattccg ttgtccggcc acgggttgtg ggaggcggag
     8941 atggagatcc ccgcgtcggc gccgagggct cgaacgaggt gggcgaccgc gggggtcggg
     9001 acgacgcccg cgaacctcac gccggcgccg gcggcgatca gcccccccga cagggcggcg
     9061 acgattcccg gagaggagac gcgggtgtcg cacccgagga cgacggtcag gggagagggg
     9121 cggccggctt cgcgcaggac ggacgcgagg gcccctccga cgcgagagac ggtggccggg
     9181 tcgagcggaa actccccggc cctaccgcgg attccgtccg ttccgaaaag cgcacgcatg
     9241 acccgtggag tttacgggag cggcggcctt cgggggtcag cggcggctcg cggccgcggc
     9301 ggcgtcgagg acgtggacga aacggagcgt ctcgtcgacg tcgtggacgc gaaggaggac
     9361 ggggttccgg tcccggagcc tctcgatcgc gaccgcgcag gcggcgagcg actccgggag
     9421 cctctcggcg gccgggcgcc ccgagacgga gccgaggaac gccttccggg aggccccgag
     9481 gacgatcggg acgccgtccg ggccgatctc ctccagccgc gcgaggagct ggaagttctg
     9541 ctcggccgac ttcccgaagc cgaggccggg atcgaggagg atctggtcgg ggcggatgcc
     9601 ggcgacgagg cagcggcgcc gggcccgggc caggtcgtgc gcgacgtcgg acacgacgtc
     9661 gggaccgaag cgagagacct cgaacgtcgt cgcgggggtc cccctcatgt ggccgaggac
     9721 gattgccgcg tcccgctcgg cgacgagctc gaggaggtcc ccggggggat cgagccccgc
     9781 cacgaggttg acgacgtccg cacccgccgc gagcgcctcc cgggcgacgg ccgttcgccg
     9841 ggtgtcgatg gagatcggga cctccgggtc gccggcccgg atcccggcga cgaccgggac
     9901 gacccgggcg gtctcctcgg cgggggagac ttctccggcg cctgtcccgt aggccgcacc
     9961 tctcggacgc gtcgactcgc ctccgacgtc gacgacgacg gccccggcgc gggcgtaccc
    10021 gagccccgcc cggaccgcgt ccccggcggc ggggaagcgc ccgccgtccg agaacgagtc
    10081 gggagtggcg ttcacgatcg ccatgacgga cgccgacggc ccgaaccgga tcgtccggcg
    10141 tccgaggcgc agctctcgag ccgacatggc cggggcgggc cggccgtcag gccggaacgg
    10201 gatcggcggg aggcgccccg tccgagacgg cgggggtcgc gccgaacgcg gagacggtcg
    10261 aggccgcgcc ccgggcggcg tcctcgggat gggacttcat cgtgtgcggg accctcttcc
    10321 cggagacctt ctcgatgagg acgtagacct cctcgccgtc gagggactcc ttctcgagga
    10381 gctcggcggc gatcgcgtcg agggcgggac ggttgtccag gacgatctgc ttgccccgca
    10441 ggtaggccga cgagatgatc cggttcacct cggcgtcgat cttccgggcg gtgtcctcgg
    10501 agtagtcggc ccttcgggcg aagtccttgc cgaggaagac ctctccctcc tcgtgcccga
    10561 acgtcatcgg gccgaggtcg ctcatccccc actcgcagac catccggcgg gccatcgtcg
    10621 tcgcctgctc gaagtcgttg gaggcgcccg tcgtgacctg gccgaggcag acctcctcgg
    10681 cgaggcggcc gcccatcatg acggcgaggc gcgcctcgac gtactccttg ttgtaggagt
    10741 acttgtcctc gaggggcagc tgctgggtga cgccgagggc gcggccgcgc gggatgatcg
    10801 tcaccttgtg gagcgggtcg gcgtgctcct cgaaggcggc gatcagggcg tgcccgcctt
    10861 cgtggtacgc ggtgttccgc ttctccttgt cggacatgac catcgacttc cgctccgccc
    10921 ccatcaggac cttgtccttg gcgtagttga agtcgtccat ctcgaccttc ttcttgtcgg
    10981 cacgggccgc cgtgagcgcc gcctcgttca cgaggttcgc gaggtcggcg ccggcgaacc
    11041 cgggcgtgcc ccgcgcgacg acggagaggt cgacgtcgtc ggacatcggg atgttcttga
    11101 cgtggacctt gaggatcccc tcgcgcccct tcacgtcggg gcggtcgacg acgacgcgcc
    11161 ggtcgaagcg gccgggccgg aggagcgccg ggtcgaggac gtcggggcgg ttcgtggcgg
    11221 cgatcaggat gacgccgtcg ttcccctcga agccgtccat ctcgacgagg agcgcgttca
    11281 gggtctgctc gcgctcgtcg tgcccgccgc cgaggcc
//
LOCUS       DAOTEZ010000096        11208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4100_length_11208_cov_6.439433, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000096 DAOTEZ010000000
VERSION     DAOTEZ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11208
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4100_length_11208_cov_6.439433"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="P5164_06925"
     CDS             complement(<1..334)
                     /locus_tag="P5164_06925"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF013495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction ATP-dependent DNA helicase
                     RuvA"
                     /protein_id="HRY43666.1"
                     /translation="MIGRLSGVVLEKRPDRALVDASGVGYELHVPLGTFAALPAVGER
                     ASLHVHTHVREDAILLFGFATAEERELFERLITVSGIGPRLALVVLSGLPLPELVGAI
                     AAQDARRLS"
     gene            complement(342..2297)
                     /locus_tag="P5164_06930"
     CDS             complement(342..2297)
                     /locus_tag="P5164_06930"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HRY43667.1"
                     /translation="MLKTFRENFKHLKWVLWAVIAVFVVFVFVDWGMGAAGGGGGGGV
                     DFAAKVGSSKITEAEFRMEYVRTEERYREMYGQSFSPELARALNLPSQVLNSLVDRRL
                     LRAEAARLGLSVSDDEVTARILRMRDAQGNLLFVRDGAFVGEATYRRMLSGANLSPEG
                     FEAETRDQVLLEKLNRFVTESTVVSEDELKADFEGRTVKAKIAYALIPAPALAPETIS
                     DADAEAHFRKDPSAWQLPERRKAKYLLVESAQLRAEVARKVTDEDVAAEYTKNAATYR
                     KDEEVAARHVLYKSDGTPASDAAARAKAEAAAKRLRGGADFAALARTESEDPGSKDSG
                     GDLGSFSRGRMVKEFEDAAFGAAVNDIVGPVKTPFGFHVIQVTAKAPARVQPLFEVAS
                     SIRARLEEERAGDEARRLARELADRVARLGKSPSDDELRKLTRPGVTFNETELLARSD
                     TPAGIGPNPVFMQQLFELPLGEVSEPVATARGEAILKPVEVKAAGPAAFADVKARAKA
                     EVARARQEELALAAARSAMTPGASVEEVAKAAGVKVETPEAFPKAGPVPGLGSGKALL
                     DAAFSAAVGETKGPVWVPERGAAVLRVLEVTPFDAAAFGAQRAEALDRLRQQKAGRLF
                     QSLVQRLRSEARIEVNRELMARFSGQA"
     gene            2435..3895
                     /locus_tag="P5164_06935"
     CDS             2435..3895
                     /locus_tag="P5164_06935"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43668.1"
                     /translation="MSRKKDGRRFEVELDWYYVSKETLWRWALVVLGAAVLVSAGIYF
                     WLNRGEDVEGRARREIAAADELLAKGRIAPGAARAREEIVAAAEKLEGARASFAAARY
                     PEALQQAVEARQLALRITAGTGAVRHDAAIMELGGRVEIQRASRANWETARVGMKLYE
                     GDFLKTGANGLAEVMAVDGTFYRIKAETLFEVHRSRVLPGGGQGAPQRQSEIKFIVGT
                     VDVDTGEGSRSIVRTDAATADIASRSAVGVDVDASKTTGVSAYRGRVTLSTESGSVTL
                     RDRERVVARVGAGGLGPKTVLPDPPRPLSPDDTAVFDLNRRQPVTLKWSPAKKDARYR
                     LQIARSRLFIPDSILIVDDRPKPEAVVTVTEEGAFYWRVATLGEGNVTSEWSPIRRFK
                     VLAGGGRASGPDTVPPELSLSRPQVNGTLVILSGRSEPGSAVFVNGEPADVDASGVFR
                     KVISFEREGVNVLTVRAVDGAGNETLRRETVMIEVY"
     gene            3946..4971
                     /locus_tag="P5164_06940"
     CDS             3946..4971
                     /locus_tag="P5164_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRY43669.1"
                     /translation="MGLRSLFSWFSSDLAVDLGTANTLVFAEGRGIVVREPSIVAVNR
                     VTNRVEAVGGAAKEMLGRTPGNIVAIRPMKDGVIADFEITEKMLDYFIKKAIGRSPFV
                     RPRIVISVPSDITQVEKRAVKDSALQAGASEVFIIEQAMAAAIGSGLPITEPTGNMIV
                     DIGGGTTDVAVISLAGIVYSKSVRVASNEMDEAIIQYIKRKYNLLIGERTAEQIKIDI
                     GSAFPLDEPLTMEIKGRDLVEGIPKTLTLTDAEVRESLAETVGILVDAVRVALEKTPP
                     ELSADIMDKGIVITGGGSLLKNLDRRLREETGLPITIAELPLDSVALGTGAMLADVDL
                     LRKISVD"
     gene            4979..5845
                     /gene="mreC"
                     /locus_tag="P5164_06945"
     CDS             4979..5845
                     /gene="mreC"
                     /locus_tag="P5164_06945"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRY43670.1"
                     /translation="MAAPLSPARPALVLATLLGSCVILLSLQVRRSEQGTLAEEWALD
                     ASAVLVRGTTGVRSAAGSLAGSFATREALREENAALRVRVAALEARLHSLHGAALERD
                     RLVRLLGAVPAPPAGTRPARIVVIDTAGPFQTALLDRGRADGVEPGGVVVGEEGLLGR
                     VVSAGERGARVQLLSDRTAATGVLLPRTGRVAVARGDGASGAAIQYVPAIADVAVGEE
                     VVTSGTDGVYPRALRLGRIAAVRRPGASLFLELPVDLAADPSREPLVFVIPPVRPDAP
                     APGAAPAGRPEP"
     gene            5842..6336
                     /locus_tag="P5164_06950"
     CDS             5842..6336
                     /locus_tag="P5164_06950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43671.1"
                     /translation="MTVALGLALAALGTVLLGVLALPHPPDLFYLPVAAVARRGRSVP
                     AMLTAFAAGLAEDALATPGRLWGLHSFTKVLVAYLLAAIGARALVEKPVLLGLLVWGA
                     VVVDGLVATLLLWILRGEPLQPVLSSLAFRAAGTGLLAAVLQAIFRHPWKERRAARRR
                     RRLA"
     gene            6348..8261
                     /gene="mrdA"
                     /locus_tag="P5164_06955"
     CDS             6348..8261
                     /gene="mrdA"
                     /locus_tag="P5164_06955"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRY43672.1"
                     /translation="MPASAREDRRPLLRRIDRLAVASFSVLALLVGIYWVHQVLRGDD
                     YARQAENNRQRSVPINASRGFILDRNGKLLAENEPSFTLLLYRRETRDVEASLRFVST
                     LLGRPLEELRRRVERLRGGYDFVPVVLDDDLSIAEVGAVEAHALEHPELVVQTTERRV
                     YTQGTVLAHLLGYLGEASPEQLASRAGRVRSGESIGQRGIEAAYQDLLAGTSGLRTFV
                     IDSFGREVAELDRLDPLPGNTLVLNVDLDLQRIADDYFRDKVGSAVALDPRTGEILAL
                     VSAPAFDPNVFSRRVTRAEWQTIIGNEDRPMQNRALQNVYSPGSVWKAFVAYAALTNG
                     IPASETAFCPGFATFYGRRFRCHGAHGTVDLPRALQVSCDVWFYNAGRRLGVDALAEA
                     ARTFGFGRPTGVDLASEKAGNVPSSAWSQSVRKHPWYPGETISVAIGQGPLLVSALQT
                     ARAFSALANEDGALPVPHLFRIGEHVRSGARVAYRPPVTERVPFPPGVRETIVEGLWR
                     VVNQPGGTAYRYRVEGLDACGKTGTVQVVAQKQAKMTHQLPEKLRDHAWFAAFAPRDD
                     AKIVVVVFVENGMHGSSAAAPLAMRLIEAHLRPGSVPVPEPPAAPAPEATPPGGTTTA
                     ANGAGAPATRTGA"
     gene            8263..9363
                     /gene="rodA"
                     /locus_tag="P5164_06960"
     CDS             8263..9363
                     /gene="rodA"
                     /locus_tag="P5164_06960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRY43673.1"
                     /translation="MSRLADALPRRVDVPLLFAALALSAAGLAMVVSTTSGTPRGSLG
                     GRQALFLLAGVVAAAFFALFDYRLLLRASPALFLASLLPLVWLPVFGPTIAGAKSWIR
                     LFGGFQVQPSELARIATVLLLAWILENDDRPYLSTRTVAALVGVVALPVGLILLQPDL
                     GVALTYVPFLVAALYLGGLRPRVWAGLVLAGSLAVGASWFVLKDYQKDRIRTFLDPDL
                     AARGAGYQVRQARIAIGSGGIAGKGWKEGTQSRLGFLPVRHSDFIFAALAEERGFLGV
                     AVVLGLYGLYLARGLGVARDARDRGGSMLVLLVVLNVAAQAAANVAMNVGVLPTTGIT
                     LPFVSYGGSSIIATWAMTGLVLSVAQRRFVNV"
     gene            9379..10932
                     /locus_tag="P5164_06965"
     CDS             9379..10932
                     /locus_tag="P5164_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013506125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HRY43674.1"
                     /translation="MQKELLISANERETRVAVLEDGRVAEVQIERRKQRGVVGNIYKG
                     RVTRVLPGMQSAFVDIGLEKDAFLYVADVGEEVEDFDRFGGMEENGAVEAEAEETGEA
                     PSAVVEAEPPPRAPLPSIDELLREGQELVVQVTKDQIAHKGVRITTHVTLPGRTLVYM
                     PTIDHVGVSRRIENEEERVRLRDILTALRKGPGGFIVRTAGEGRAADEFASDKRYLAR
                     LWEQIRKRAERSGAPALLHRDLDLTLRAARDIFGQEFAVLWVDDDEEYRRIVEFLDQV
                     QPNLVGKVKLYRKSSPLFEELGVDAEIENALKSKVWLKSGGYIVINQTEALVAIDVNT
                     GKYIGKKRLEDTVLRTNMEAVQEIVRQIRLRDLGGLLVVDFIDMEEEEHRQELVTALE
                     AELKKDRSKNRMLPVSEFGLVEITRKRQRQSLERALTMPCPYCSGSGRIKSVPTILLE
                     IRREILKRAASGEGHAIFLRVHPEISRALSSDERHLIEELTEHVGMPIHVQVDEHLHH
                     EGYDVVVEA"
BASE COUNT         1527 a         4209 c         3858 g         1614 t
ORIGIN      
        1 aggagagccg cctcgcgtcc tgcgcggcga tcgctccgac gagctcgggc agcggaaggc
       61 ccgagaggac gacgagcgcc aggcgcgggc cgatcccgga gacggtgatc agcctctcga
      121 agagctccct ctcctcggcc gtcgcgaagc cgaagaggag gatcgcgtcc tcgcggacgt
      181 gggtgtggac gtggaggctc gcgcgctccc cgacggcggg gagcgcggcg aacgtcccga
      241 gcggcacgtg cagctcgtag ccgacgcccg aggcgtcgac gagggcccgg tcgggccgct
      301 tctccaggac gactcccgag aggcgcccga tcacgtcccg gtcaggcctg cccggagaag
      361 cgggccatca gctcccggtt gacctcgatc cgcgcctcgg aacggagcct ctggacgaga
      421 gactggaaca gccgccccgc cttctgctgg cgcagccggt ccagcgcctc ggcccgctgc
      481 gccccgaacg cggcggcgtc gaacggggtc acctcgagga cgcggaggac cgcggcgccc
      541 cgctcgggga cccagaccgg gcccttcgtc tcgccgacgg cagccgagaa ggccgcgtcg
      601 aggagcgcct tgccggagcc gagccccggc accggccccg ccttcgggaa cgcctcgggc
      661 gtctccacct tcacgccggc cgccttcgcg acctcctcga ccgacgcgcc cggcgtcatc
      721 gcggagcgcg cggcagcgag ggccagctcc tcctgcctcg cgcgggcgac ctcggctttc
      781 gcccgcgcct tcacgtcggc gaacgcggca ggccccgccg ccttcacctc gacgggcttc
      841 aggatcgcct cgccccgcgc ggtggcgacc ggctcggaga cctcgccgag cggaagctcg
      901 aagagctgtt gcatgaagac cgggttcggg ccgatcccgg ccggggtgtc ggagcgggcc
      961 aggagctcgg tctcgttgaa cgtcacgccg gggcgggtca gcttccggag ctcgtcgtcg
     1021 gacggcgact tgccgagccg cgcgacgcgg tcggcgagct cgcgcgccag gcgccgcgcc
     1081 tcgtcccccg cacgctcctc ctcgagccgc gcccggatcg acgaggcgac ctcgaacagg
     1141 ggctggacgc gcgccggggc cttcgcggtc acctggatca cgtggaagcc gaacggcgtc
     1201 ttgacggggc cgacgatgtc gttcacggcc gcgccgaacg cggcgtcctc gaactccttc
     1261 accatccgcc cgcgggagaa cgagccgagg tcgcccccgg agtccttcga cccggggtcc
     1321 tcggattcgg tccgcgcgag ggcggcgaag tcggctccgc cccggaggcg cttcgcggcc
     1381 gcttcggcct tcgcccgcgc ggcggcgtcg gaggctggcg tcccgtcgga cttgtagagg
     1441 acgtgacggg ccgcgacctc ctcgtccttg cggtacgtcg cggcgttctt cgtgtactcg
     1501 gcggcgacgt cctcgtcggt gaccttccgg gcgacctcgg cgcggagctg cgcgctctcg
     1561 acgaggaggt atttcgcctt gcggcgctcg ggaagctgcc aggccgacgg gtccttacgg
     1621 aagtgcgcct ccgcgtcggc gtcggagatc gtctcgggcg cgagcgccgg ggccgggatc
     1681 agggcgtagg cgatcttcgc cttcacggtc cgcccctcga agtccgcctt cagctcgtcc
     1741 tcggagacga ccgtcgactc ggtcacgaac cggttcagct tctccaggag gacctgatcg
     1801 cgcgtctcgg cctcgaagcc ttcgggcgag aggttcgctc cggagagcat ccgccggtag
     1861 gtcgcctcgc cgacgaacgc cccgtccctc acgaagagga ggttcccctg cgcgtcgcgc
     1921 atccggagga tgcgtgccgt cacctcgtcg tcggagacgg agaggccgag ccgggcggcc
     1981 tccgcgcgga ggagacgccg gtcgacgagg gagttgagca cctgggaggg aaggttgagc
     2041 gcgcgggcga gctccgggct gaagctctgc ccgtacatct cccggtaccg ctcctcggtc
     2101 ctcacgtact ccatccgaaa ctcggcttcg gtgatcttcg aggagccgac cttcgcggcg
     2161 aagtcgacgc cgcccccgcc tcctcccccg gccgccccca tcccccagtc gacgaagacg
     2221 aagacgacga agaccgcgat gacggcccaa aggacccact tgaggtgctt gaagttttcc
     2281 ctgaacgtct tgagcattcc ctctccgagc cggcgccggt ctcccggcgc cgcaagcgca
     2341 ggaggatagc gcgggcgttc cggcccgaca agccgagcgg aagggcgtgg tataaatgga
     2401 agttcgctgg taagcggctg aaggcccgaa gtttatgtcg aggaagaagg acggccgccg
     2461 gttcgaggtc gagctcgact ggtactacgt ctccaaggag acgctctggc gctgggcgct
     2521 cgtcgtcctc ggcgcggcgg tcctcgtctc ggccggcatc tacttctggc tcaaccgggg
     2581 cgaggatgtc gagggacgcg cgcgccgcga gatcgccgcc gccgacgagc tcctcgccaa
     2641 ggggcgcatc gcccccggcg ccgcgcgggc ccgggaggag atcgtcgccg ccgccgagaa
     2701 gctcgagggc gcccgcgcgt cgttcgccgc ggcacgctat cccgaggccc tccagcaggc
     2761 ggtcgaggcc cggcagctcg cgctccggat cacggccggg acgggggccg tccggcacga
     2821 cgccgccatc atggagctcg ggggacgcgt cgagatccag cgcgcgagcc gcgcgaactg
     2881 ggagacggcc cgcgtcggga tgaagctcta cgagggggac ttcctgaaga ccggcgcgaa
     2941 cggcctcgcc gaggtgatgg ccgtcgacgg caccttctac cggatcaagg ccgagacgct
     3001 cttcgaggtc caccggagcc gggtcctccc cggggggggg cagggcgccc ctcagcgcca
     3061 gtccgagatc aagttcatcg tcggcaccgt cgacgtcgac accggcgagg gctcccgctc
     3121 gatcgtccgg accgacgcgg cgacggcgga catcgcctcg cggtcggcgg tcggcgtcga
     3181 cgtcgacgcg tccaagacga ccggcgtctc ggcctaccgc ggccgggtga ccctctccac
     3241 cgagagcggg tcggtgactc tccgggaccg cgagcgggtc gtcgcacgcg tcggcgccgg
     3301 gggcctcggc ccgaagaccg tgctccccga ccccccgcgt ccgctctcgc ccgacgacac
     3361 cgccgtcttc gacctgaacc gccgccagcc ggtgacgctg aagtggtcac cggcgaagaa
     3421 ggacgcgcgc taccgcctcc agatcgcgcg gagccgcctc ttcatccccg actcgatcct
     3481 catcgtcgac gaccgtccga agccggaggc cgtcgtcacc gtcacggagg agggggcctt
     3541 ctactggcgc gtcgcgacgc tcggcgaggg aaacgtcacc tccgagtggt cgccgatccg
     3601 ccgattcaag gtcctcgcgg gcggagggcg ggcgagcggg cccgacacgg tcccccccga
     3661 gctctccctc tctcgcccgc aggtaaacgg gacgctcgtc atcctctcgg gccgctccga
     3721 gcccggctcg gcggtcttcg tcaacggcga gcccgccgac gtcgacgcct ccggggtctt
     3781 ccgcaaggtg atctccttcg agagggaagg ggtcaacgtc ctcacggtcc gcgccgtgga
     3841 cggggccggg aacgagaccc tccggcggga gacggtcatg atcgaggtct actagcccga
     3901 aactcgcgcc ccgcggggcg ctcacgacgc cgaaaggaac ctcccttggg cctgcgctct
     3961 ctcttttcgt ggttctcctc ggacctggcg gtcgacctcg ggaccgcgaa cacgctcgtc
     4021 ttcgccgagg gccggggcat cgtcgtccgc gagccgtcca tcgtcgccgt caaccgcgtc
     4081 acgaacaggg tcgaggcggt gggcggcgcg gcgaaggaga tgctcggccg gacgcctggg
     4141 aacatcgtcg cgatccgccc gatgaaggac ggcgtcatcg ccgacttcga gatcaccgag
     4201 aagatgctcg actacttcat caagaaggcg atcggccgct cgccgttcgt gaggccgcgc
     4261 atcgtcatct cggtcccgtc cgacatcacg caggtcgaga agcgcgcggt gaaggactcg
     4321 gcgctccagg cgggtgcgag cgaggtcttc atcatcgagc aggcgatggc ggccgcgatc
     4381 ggctcggggc tcccgatcac cgagccgacc gggaacatga tcgtcgacat cggcggcggg
     4441 acgaccgacg tcgcggtgat ctcgctcgcc ggcatcgtct actcgaagtc ggtgcgcgtc
     4501 gcctcgaacg agatggacga ggcgatcatt cagtacatca agaggaagta caacctcctc
     4561 atcggcgaga ggacggccga gcagatcaag atcgacatcg ggtcggcctt cccgctcgac
     4621 gagccgctca cgatggagat caaggggcgc gacctcgtcg aagggatccc gaagacgctc
     4681 acgctcaccg acgccgaggt gcgcgagtcg ctcgcggaga cggtcggcat cctcgtcgac
     4741 gccgtccgcg tcgcgctcga gaagaccccc ccggagctct cggccgacat catggacaag
     4801 gggatcgtca tcaccggagg cggctcgctc ctgaagaacc tcgaccggcg cctccgcgag
     4861 gagaccgggc tcccgatcac gatcgccgag ctcccgctcg actcggtcgc gctcgggacc
     4921 ggggcgatgc tcgcggacgt cgacctgctc cggaagatct ccgtcgactg aggccccggt
     4981 ggccgctccg ctctcccccg cacggccggc gctcgtcctc gcgacgctgc tcggcagctg
     5041 cgtgatcctc ctctccctgc aggtgaggag gtcggagcag gggacgctcg cggaggagtg
     5101 ggccctggac gcctccgccg tcctcgtgcg cgggacgacg ggggtccgtt ccgccgccgg
     5161 ctcgctcgcc gggagcttcg cgacgcgcga ggcgcttcgg gaggagaacg ccgcgctccg
     5221 cgtgcgggtc gccgccctcg aggcgcgcct ccattccctg cacggggcgg cgctggagcg
     5281 ggaccggctc gtgcggctcc tcggtgcggt ccccgcgccg ccggccggga cccgccccgc
     5341 gcggatcgtc gtcatcgaca cggccggccc gttccagacc gccctgctcg accgcggccg
     5401 cgccgacggc gtcgagccgg gcggcgtcgt cgtcggcgag gaaggcctgc tcggccgggt
     5461 cgtctccgcc ggggagcgcg gcgcgcgcgt ccagctcctc tccgaccgga cggccgccac
     5521 cggcgtcctc cttccgcgga cgggccgcgt cgcggtggcc cgcggcgacg gtgcctcggg
     5581 ggccgcgatc cagtacgtcc cggccatcgc ggacgtcgcg gtcggcgaag aggtcgtcac
     5641 gtccgggacc gacggcgtct acccgcgagc gctccgtctc gggcggatcg ccgccgtccg
     5701 ccgccccggc gcctccctgt tcctggagct cccggtcgac ctcgccgctg acccgtcgcg
     5761 cgagccgctc gtcttcgtca tcccgcccgt ccgccccgac gccccggcgc cgggggccgc
     5821 gcccgccgga cgtcccgagc cgtgaccgtc gcgctcggcc tcgccctcgc cgccctcgga
     5881 accgtcctgc tcggagtcct cgccctcccc cacccgccgg acctcttcta cctgccggtc
     5941 gcggcggtgg cgcgccgcgg gcggtcggtc cccgcgatgc tgaccgcctt cgccgcgggg
     6001 ctcgccgagg acgccctcgc cacgccgggc cggctctggg gcctccactc gttcacgaag
     6061 gtcctcgtcg cctatctcct cgcggcgatc ggggcgcgcg ccctcgtgga gaagcccgtc
     6121 ctgctcggtc tcctggtctg gggcgccgtc gtcgtcgacg gcctcgtcgc gacgctcctc
     6181 ctctggatcc tgcgcggcga gccgctccag cccgtcctct cgtcgctcgc gttccgggcc
     6241 gccgggaccg ggctcctcgc cgccgtcctc caggcgatct tccgccatcc ctggaaggag
     6301 cgccgcgcgg cgcgccgccg tcgaaggctg gcatagtcgg agacccgatg cccgcaagcg
     6361 cccgcgagga ccgccgcccc ctccttcgcc ggatcgaccg gctcgccgtg gcgtcgttct
     6421 ccgtcctcgc cctgctcgtc ggcatctact gggtccacca ggtcctccgc ggggacgact
     6481 acgcccgcca ggccgagaac aaccgtcaga ggtcggtccc gatcaacgcc tcgaggggct
     6541 tcatcctcga ccggaacggg aagctcctgg cggagaacga gccgtccttc acgctcctcc
     6601 tctaccggcg ggagacgcgc gacgtcgagg cctccctccg tttcgtatcg accctcctcg
     6661 gccggccgct cgaggagctc cgccgccgcg tcgagaggct tcgcgggggc tacgacttcg
     6721 tgccggtcgt cctcgacgac gacctgtcga tcgccgaggt cggcgccgtg gaggcgcacg
     6781 cgctcgagca ccccgagctc gtcgtccaga cgaccgagcg ccgcgtctac acgcagggga
     6841 cggtcctcgc ccacctcctc ggatacctcg gggaggcgag ccccgagcag ctcgcctcgc
     6901 gcgccggacg cgtccgctcg ggcgagtcga tcggccagcg cgggatcgaa gcggcttacc
     6961 aggaccttct cgccgggacg tcgggcctgc gcacgttcgt catcgactcc ttcggacgcg
     7021 aggtggccga gctcgaccgg ctcgacccgc tgcccgggaa cacgctcgtc ctgaacgtcg
     7081 acctcgacct ccagcggatc gccgacgact acttccgcga caaggtcgga tcggcggtcg
     7141 cgctggaccc gaggaccggc gagatcctcg ccctcgtctc cgcgccggcc ttcgacccga
     7201 acgtcttctc ccggcgggtc acgcgggcgg agtggcagac gatcatcggg aacgaggacc
     7261 gcccgatgca gaaccgggcg ctccagaacg tctactcgcc gggctccgtc tggaaggcct
     7321 tcgtcgcgta cgccgcgctg acgaacggga ttcccgcctc cgagaccgct ttctgccccg
     7381 gtttcgccac gttctacggc cgccgcttcc gctgccacgg cgcgcacggc acggtcgacc
     7441 tgccgcgggc gctccaggtc tcgtgcgacg tctggttcta caacgcgggc cggaggctcg
     7501 gcgtcgacgc cctcgccgag gccgcgcgga ccttcgggtt cgggcgcccg accggcgtcg
     7561 acctcgcctc cgagaaagcc gggaacgtcc cctcctcggc ctggagccag tcggtgcgca
     7621 agcatccctg gtacccgggc gagacgatct ccgtcgccat cggccagggg ccgctgcttg
     7681 tctcggcgct ccagacggcg cgcgccttct cggcgctcgc gaacgaggac ggcgcccttc
     7741 cggtgccgca cctgttccgg atcggggagc acgtccggag cggcgcccgc gtcgcgtacc
     7801 gtccgcccgt caccgaaagg gtcccgttcc ccccgggcgt gagggagacg atcgtcgagg
     7861 gactctggcg cgtggtgaac cagccgggcg ggacggccta ccgctaccgg gtggaaggcc
     7921 tcgacgcctg cgggaagacc gggaccgtcc aggtggtcgc ccagaagcag gcgaagatga
     7981 cgcaccagct ccccgagaag ctgcgcgatc acgcctggtt cgccgccttc gccccgcgcg
     8041 acgacgcgaa gatcgtcgtc gtcgtcttcg tcgagaacgg catgcacggc tcctcggccg
     8101 ccgcccccct cgcgatgcgt ctcatcgagg cgcacctgcg ccccgggagc gtccccgtcc
     8161 cggagccgcc ggccgcgccc gccccggagg cgacccctcc cgggggaacg acgacggccg
     8221 cgaacggcgc cggcgcgccc gcgacccgga cgggggcctg acgtgtcgcg cctcgccgac
     8281 gcgctcccgc ggcgagtcga cgtgccgctc ctcttcgccg cgctcgccct gtcggccgcc
     8341 ggcctcgcga tggtcgtctc gacgacctcg gggacgcccc gcgggagcct cggtggacgc
     8401 caggcgctgt tccttctcgc cggggtggtc gcggcggcct tcttcgccct cttcgactac
     8461 cggctcctgc tccgcgcctc gcccgcgctc ttcctcgcct cgctccttcc gctcgtctgg
     8521 ctccccgtct tcgggccgac gatcgcggga gcgaagtcct ggatccggct cttcggcggg
     8581 ttccaggtcc agccgtccga gctggcacgg atcgcgaccg tcctcctcct cgcgtggatc
     8641 ctcgagaacg acgaccgccc ctacctctcg acgcggaccg tcgccgccct cgtcggcgtc
     8701 gtcgcgctcc cggtcggcct catcctcctg cagcccgacc tcggggtcgc gctcacctac
     8761 gtcccgttcc tcgtcgccgc cctctacctc ggaggccttc gcccgagggt ctgggccggg
     8821 ctcgtcctcg ccggaagcct cgccgtcggg gcctcctggt tcgtcctcaa ggactaccag
     8881 aaggaccgga tccggacctt cctcgacccg gacctcgccg cgcgcggagc cggctaccag
     8941 gtgcggcagg cgcgcatcgc gatcgggtcg ggcggcatcg ccgggaaagg gtggaaggaa
     9001 gggacgcaga gccgcctcgg cttccttccg gtccgccact ccgacttcat cttcgccgcg
     9061 ctcgccgagg agcgcgggtt cctcggcgtc gccgtcgtcc tcggcctcta cggcctctat
     9121 ctcgcgaggg ggctcggcgt agcgcgcgac gcgcgcgacc ggggcggctc gatgctcgtc
     9181 ctcctcgtcg tcctcaacgt cgcggcgcag gccgcggcga acgtcgccat gaacgtcgga
     9241 gtcctgccga cgacgggcat caccctgccg ttcgtctcct acgggggctc gtccatcatc
     9301 gccacctggg cgatgacggg gctcgtcctc tccgtcgccc agcgccggtt cgtcaacgtc
     9361 tgaatgcccg aggcccacat gcagaaagag ctcctcatct ccgccaacga acgcgagacg
     9421 cgggtcgccg tcctcgagga cggccgggtc gcggaggtcc agatcgagcg gcgcaagcag
     9481 cgcggcgtcg tcgggaacat ctacaagggg cgcgtcacgc gcgtcctgcc ggggatgcag
     9541 tcggcgttcg tcgacatcgg cctcgagaag gacgccttcc tctacgtcgc cgacgtcgga
     9601 gaggaggtcg aggacttcga ccgcttcggc ggtatggagg agaacggcgc cgtcgaggcc
     9661 gaggcggagg agaccggaga ggctccgtcc gcggtcgtcg aggccgagcc tcccccgcgc
     9721 gcgccgctcc cctcgatcga cgagctcctc cgcgaagggc aggagctcgt cgtccaggtg
     9781 acgaaggacc agatcgccca caagggcgtg aggatcacga cgcacgtcac ccttcccggc
     9841 cggacgctcg tctacatgcc gacgatcgac cacgtcggcg tctcgcgccg gatcgagaac
     9901 gaggaggagc gggtccgcct ccgcgacatc ctcaccgctc tccgcaaggg gccgggcgga
     9961 ttcatcgtcc ggacggcggg cgaggggcga gccgccgacg agttcgcctc cgacaagcgc
    10021 tacctcgccc ggctctggga gcagatccgc aagcgggcgg agcgctccgg cgcccccgcc
    10081 ctccttcacc gcgacctcga cctgacgctc cgggcggcgc gcgacatctt cggccaggag
    10141 ttcgccgtcc tctgggtcga cgacgacgag gagtaccgcc ggatcgtcga gttcctcgac
    10201 caggtccagc cgaacctcgt cggcaaggtg aagctctacc ggaagtcgag cccgctcttc
    10261 gaggagctcg gcgtcgacgc ggagatcgag aacgccctga agtcgaaggt ctggctgaag
    10321 tcgggcggct acatcgtcat caaccagacc gaggcgctcg tcgccatcga cgtcaacacc
    10381 gggaagtaca tcgggaagaa gcggctcgag gacacggtcc tccggacgaa catggaggcc
    10441 gtccaggaga tcgtgaggca gatccggctc cgcgacctcg gcggcctcct cgtggtggac
    10501 ttcatcgaca tggaggagga ggagcaccgg caggagctcg tcacggcgct cgaggccgag
    10561 ctgaagaagg accgctccaa gaaccggatg ctcccggtct ccgagttcgg cctcgtcgag
    10621 atcacgcgga agcggcagcg ccagagcctc gagcgggcgc tcacgatgcc ttgcccctat
    10681 tgctcgggct cgggccggat caagagcgtc ccgacgatcc tcctcgagat ccgccgcgag
    10741 atcctcaagc gggccgcctc gggggagggc cacgccatct tcctccgggt ccacccggag
    10801 atctcgcggg cgctctcctc ggacgagcgc cacctgatcg aggagctcac cgagcacgtc
    10861 gggatgccga tccacgtcca ggtcgacgag cacctgcacc acgaggggta cgacgtcgtc
    10921 gtggaagcgt gacccggatc acccccgtgc cgccggggca ccccctcccc cgacgtctcg
    10981 ggcgggaaac gggtccgaaa ctggtcgaaa tggcacggag aggactggca cgccgcctgc
    11041 attaagaagg gcggcgcaca ctccagcctc tcctcccagc cgccccgatg ggcggctctt
    11101 ttttttggga caatggcttc gtggctcacg ccacggtgcg ctccgggtat cgagacctcg
    11161 tcgagcggct gaaccgcttt ccacagggtg cgcctccgtc ggatctcc
//
LOCUS       DAOTEZ010000097        11104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4153_length_11104_cov_6.649742, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000097 DAOTEZ010000000
VERSION     DAOTEZ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11104
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4153_length_11104_cov_6.649742"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..3437)
                     /locus_tag="P5164_06970"
     CDS             complement(132..3437)
                     /locus_tag="P5164_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRY43675.1"
                     /translation="MTAPLVDDAARERARTDVGTSLLVEASAGTGKTKTLVDRIVCLV
                     LDEGEPLSTVAALTFTEKAAGEMKARLRTRLDEEARAATDAARRERALRARRDLDGAE
                     VTTIHSFCARLLRERPVEARVDPDFVVPEDGVAEELFDDVFAAWVDEEARAGGFVTDA
                     LRRGLSPDTLRDLARTIRSERTLVLAGTIPDDPLPYFRLELASLADAGDRAVEEFPSD
                     LRTDAKALLLRSAVEEVRALLPLDLDGLASARLETKVDFRVGKRGVYAGRLKELADAF
                     RDEWKELPDRLASLSDERLLARVAASIRGGLLPRLASRMRERGLLDFDDLLLAARDLL
                     RTSRAAREHFQDRFRHLVVDEFQDTDPVQAEIVLRISCQDGEQDEEHWDGLVPAPSAL
                     FLVGDPKQSIYRFRRADVEVYRQVADRLPSSDRLSLVTNFRSSPRILDFANGLFESVF
                     EPAPGAPWEVAHAAFAPDPEKLARPDDGRVLFLAAPEVPDLHDGGEDGRSAAEAEGGD
                     DGLDAEVKLQEARAVANLLLSRCSEGGFRGTAVLLGSNDAIDLYQEVFRGAGIPAVLE
                     GGVSFYRREETAAVVAALRAIDDPADSISTVAALKSFLFGLTDVELLDASEAGTLFDD
                     PSTAPADGPVGEALGALAALRARRHARPLAETLLDLLSSSSALAAVENGAVVNPLQAA
                     ANLDRLLVLARALDAEGLPFREAVARLALRLEEKVSEPRAFEEDEDAVRLMTLHKAKG
                     LEFDTVVVASLGFRDRERTAGRRTLVHTGPGRPLALSASMRGRTVRTPGFRSIALLDD
                     ARELAEEKRQLYVALTRAKRTLVLSWFRRRRRLKSGDVSDTIGKSLLAPIAFAEPLPA
                     RLAPLVEVVEPDVSTPPSPRTAPAPAFTVDLDAERTAAEARLARARATATRPLRRSGE
                     KDAPWTPRPPDGEDAGSPEDLPSLERRDEVHEAAVRLGTAVHEAMEALLDPAHMPPAS
                     AAEAVATSALPLDPAARAEAVRLVERLIDHPVVARARAARRRFVELPILFRDDALENA
                     PLVEGKIDLLFEEEDGWVVVDWKTDRLPTPAVRAEREALYAPQLASYARAVVALLGPG
                     TRVKETLLAFARG"
     gene            complement(3434..6559)
                     /locus_tag="P5164_06975"
     CDS             complement(3434..6559)
                     /locus_tag="P5164_06975"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRY43676.1"
                     /translation="MGGSPRHTLCTGGFAALEARFLEELRERKSGDPLRPVDVLVGSN
                     LLGVYMRRRAAETLGGIANLRLLTFVDLARERVPAADPRPPLPSVGEALLSRRALLET
                     PEGAEFGDLRERPSTAGLVARTAADLRGAGLAAGSLRALLPAAARTPDRAAFLGNVGA
                     VLARYEELRSAFADADALLARAAAVKVPPRPDPLLVYGLYDLGGVREALLAAVAAERP
                     VVAFVPVDGEPEVGAEAADAVAPRVRAALFQGLLGVPPRDAGPAPAPPEVCVVVAPSE
                     LSEAREVVREVLCAVDDGVPLHRIAVLVRDPARQEPALVAELSARRIPFFRPAGAGFS
                     RTPLGCAALGLLALPAGGFAPDDLVALVDVLEGLALLPPGVRARVERALLEIRFRDGW
                     AGIVPRIEARLARRPPEEAAEAEGRVTRREMRVRRDLAALREVVALLAPVLPDPAPAP
                     WRTWSERLAASFDALFGRLPGAGELSGAATRLGELEAVEPGVPVAAIDVLPLLADSLE
                     ETPVRQGRFERDGLSLLSSVSARGLLFDVVLVPGLVEQSFPRAGRPDPLLFDDERAAL
                     SRATRRPIAPRTGPRHAREERFLFHLARSAARRRLVLLAARREAATDRPRLLSPFLLD
                     HLEERAGRSFSEAELAKPELAAPLSLRWIRLGAPATAEPPLDADDALRRALARAPKLR
                     SALPPGLETLRRALRRGRARAEARYTEYEGRLSRAPLRLGLGGGRVLSPSRLERLASC
                     AYRAFLTDALGLSAPEEAPAELALDGRTTGELAHAALEAAAKSALEREVPLGDLLLSQ
                     ADALAARAVTRFLLDWELDLPPVLVEVATEQLAASLRAVGELESSRPDPLPLAGGEIG
                     FGPGRGAEPAAGEDPLRVPLTGRIDRLDRRGETARVVDYKFSRPDPFGAKNRKGWRIV
                     GGEKVQLAAYALAARALGAGSVSSEYLFVTADRKGAEPTALPVAFDAAETGEAVASFH
                     RAVGLLDATVRKGDLLPRTESLSSRGSPCSFCDVAAVCGPGHARVYATKREAERLADP
                     AAPLFALEEIP"
     gene            complement(6596..7171)
                     /locus_tag="P5164_06980"
     CDS             complement(6596..7171)
                     /locus_tag="P5164_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014516498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HRY43677.1"
                     /translation="MADPPPRRVVVGISGASGIPYAKDLLETLRRIGGLEVHLVATDG
                     ARRVAADELGAPLDDLAALADVVHPNRDVGASIASGSFRTLGMVVVPCSATTLSKVAY
                     GVCDNLLTRAAYVHLKERRPLVLVPREAPLPLPTLEAMVKAAQAGAAILPASPGFYTR
                     PKTIDDLLGFVSQRVLDLLGIEGDRSPRWKD"
     gene            complement(7183..7401)
                     /locus_tag="P5164_06985"
     CDS             complement(7183..7401)
                     /locus_tag="P5164_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008633749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43678.1"
                     /translation="MTRVLFVEGKDADALRALAARLDVPWRLLVRPEQGLFLLEATGP
                     PPEVEREAASLPGLRAWAFDVVDARRSD"
     gene            7438..7536
                     /locus_tag="P5164_06990"
     tRNA            7438..7536
                     /locus_tag="P5164_06990"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7472..7474,aa:Sec,seq:tca)
     gene            complement(7547..8089)
                     /locus_tag="P5164_06995"
     CDS             complement(7547..8089)
                     /locus_tag="P5164_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43679.1"
                     /translation="MIVFLLLLAQASAPAPTPAPTARPAPALGRSGPAARPKTLSEHA
                     AEMKAKGTPASRVSFEDLPSVEGDPPEADPEPVAGARTAAGPTGKADEAAARAAQKRM
                     DRAVERGLEVPERTRSPRRDNARKEWDDAAESCRQTPGCVPVYRDDPRWGDKKPLKTD
                     AELIEEIRRRGFSEPHPIRK"
     gene            8193..9563
                     /locus_tag="P5164_07000"
     CDS             8193..9563
                     /locus_tag="P5164_07000"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRY43680.1"
                     /translation="MDSSARGGTGKRMTSEERNRLLVRIVNELDGLLNLESVLGRLLD
                     TLRTAVGYDAAGIFVLGRDEAFPGWGSSWRTIAASARRGFDEPMPDVDRLLALGHGLV
                     GHVLRTGEPAVAPDVLADPRYVMGRPSTRSELAVPVCAGGRLIGAMNLESDEPRAYSE
                     EDAEPLRLFADAAAIAVEKAVLYRLFVQRERLEEELRGEVELQAAAGSAAWRDLPGWD
                     AVGFTRPRYEIGGDYYDRIPLGDGRLGLVVADVSGKGSLAAMTVATFRAILRAQVDGG
                     GEPVRTATAANRLLRERVRPTEYVTAVWGVLDPARGLLSYTNCGHVPPLLVRADGSYT
                     RLTTGGPPLGLLASARYLSGEVSIGPGDLLLLFTDGVVESEGPAEAEYGADRLAEAAR
                     DGRGLPSAELVEAIVRSVAEFRGADEPTDDFTLLVLKRTGEAASPPDVRATHCTPGRE
                     PGLEVP"
     gene            9560..10246
                     /locus_tag="P5164_07005"
     CDS             9560..10246
                     /locus_tag="P5164_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRY43681.1"
                     /translation="MRRLAHLVLLALAGPLLGQMAPPAEPGPFRETDLVELTALDPTI
                     RLDVKYATNDNFVGRPVYREARAFLQRPAAEALVRAHRKLGEKGFGLVVFDGYRPWAV
                     TKAFWDATPDEKKVFVADPRRGSRHNRGCSVDVALYDRATGKTVDMGGAYDEFGPRSY
                     VTWEGGTKEQLEKRDLLRGAMEREGFFVYPWEWWHFDFKDWNAFPILDVPFEKLGKAA
                     GPAAGPAKAP"
     gene            10270..>11104
                     /locus_tag="P5164_07010"
     CDS             10270..>11104
                     /locus_tag="P5164_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRY43682.1"
                     /translation="MAHRILICDDEGDIRESLRAILLDEGFDVTAVAAGPNAVAESPD
                     HDALLLDIKMPGQDGLETLAQIRKRGVATPVIMISGHGDVKTAMEAIRAGADDFLEKP
                     LATEHVLNALRRVLDASRLTRENQELRSRLGLTRLVGDAPVMKKLVADVQRVAPTPAT
                     ILIVGPSGTGKELVARSVHEGSAVRGGPFIQVNCAAIPETLIESELFGHEKGSFTGAE
                     RKQVGKFVEADGGTIFLDEIGDMSLSAQAKVLRVLQEGEVEPVGSPRVVRVKVRVIAA
                     TN"
BASE COUNT         1480 a         3876 c         4444 g         1304 t
ORIGIN      
        1 tggccgacct cgcgacgaac gtcgcggagg agacgatctt catcgccgag gggctggtca
       61 tcaagcacca ggggacccgc ccggaggagc cggcggggcg ctgaggaggc ggagccggcg
      121 ggccgggccc gtcagcctcg cgcgaaggcg agcagcgtct ccttcacgcg cgtccccggt
      181 ccgagcagag cgaccaccgc ccgggcgtag ctcgcgagct gcggcgcgta gagcgcctcg
      241 cgctcggcgc ggacggccgg cgtcgggagg cggtcggtct tccagtccac gacgacccag
      301 ccgtcctcct cctcgaacag gaggtcgatc ttcccctcga cgagcggcgc gttctcgagc
      361 gcgtcgtcgc ggaagaggat cggcagctcg acgaagcgcc gccgagcggc gcgggcgcgg
      421 gcgacgacgg ggtggtcgat caggcgctcg acgagccgaa cggcctcggc gcgcgcggcc
      481 ggatcgagcg gaagcgccga ggtcgcgacg gcctcggccg cggaggcggg gggcatgtgc
      541 gcggggtcga ggagggcctc catcgcctcg tggacggccg tgccgaggcg cacggcggct
      601 tcgtggacct cgtcgcggcg ctcgagcgac gggaggtcct cgggcgatcc ggcgtcctcg
      661 ccgtccggcg gacgcggcgt ccagggggcg tccttctcgc cggagcggcg gagcgggcgg
      721 gtggccgtgg cccgggcccg cgcgaggcgg gcctcggcgg cggtcctctc ggcgtcgagg
      781 tcgacggtga acgccggggc gggcgccgtc ctcggagacg gaggcgtgga gacgtcgggc
      841 tcgaccacct cgacgagggg ggcgaggcgc gcagggagcg gctcggcgaa cgcgatcggc
      901 gcgaggaggc tcttgccgat cgtgtccgac acatccccgg acttcagccg gcggcgcctc
      961 cggaaccagg agaggacgag cgtcctcttc gcccgcgtca gcgcgacgta gagctgccgc
     1021 ttctcctcgg cgagctcgcg ggcgtcgtcg aggagcgcga tggagcggaa cccgggagtc
     1081 cggaccgtcc gcccgcgcat cgacgcggaa agcgcgagcg gcctgccggg gccggtgtgg
     1141 acgagcgtcc ggcggccggc cgtccgctcg cggtcgcgga agccgaggct cgcgacgacg
     1201 accgtgtcga actcgagccc cttcgccttg tggagcgtca tgaggcggac ggcgtcctcg
     1261 tcctcctcga acgcgcgcgg ctcggagacc ttctcctcga ggcggagcgc caggcgggcg
     1321 acggcctcgc ggaacgggag cccctccgcg tcgagcgcgc gggcgaggac gaggaggcgg
     1381 tcgaggttcg ccgcggcctg gagggggttg acgacggccc cgttctcgac ggcggcgagg
     1441 gccgagctcg acgagaggag gtcgaggagc gtctcggcca ggggacgcgc gtgccggcgc
     1501 gctcggagcg ccgccagggc cccgagcgct tcgccgacgg ggccgtccgc cggcgcggtc
     1561 gacgggtcgt cgaagagggt gcccgcctcg gaggcgtcga ggagctcgac gtcggtgagg
     1621 ccgaagagga aggacttcag cgcggcgacc gtggagatcg agtcggccgg gtcgtcgatc
     1681 gcgcggagcg cggcgacgac cgccgcggtc tcctcgcgcc ggtagaagct gacgcccccc
     1741 tcgaggacgg ccgggatccc ggccccgcgg aagacctcct ggtagaggtc gatcgcgtcg
     1801 ttcgagccga ggaggacggc cgtcccgcgg aagcccccct cggagcagcg cgaaaggagg
     1861 aggttcgcga cggcgcgcgc ctcctggagc ttcacctccg cgtcgaggcc gtcgtcgccg
     1921 ccctcggcct cggcggcgga cctcccgtcc tcgccgccgt cgtggaggtc gggcacctcg
     1981 ggcgccgcga ggaagaggac ccgtccgtcg tcgggccggg cgagcttctc cgggtcgggc
     2041 gcgaacgccg cgtgcgcgac ctcccagggc gcgcccggcg caggttcgaa cacgctctcg
     2101 aagaggccgt tcgcgaagtc gaggatgcgg ggcgaggagc ggaagttcgt gacgagcgag
     2161 agccggtcgg aggaggggag gcggtcggcg acctgcctgt agacctccac gtcggcgcgg
     2221 cggaagcggt agatcgactg cttcgggtcg ccgacgagga agagtgcgga cggagcgggg
     2281 acgaggccgt cccaatgctc ctcgtcctgc tcgccgtcct ggcaggagat ccggaggacg
     2341 atctcggcct ggaccgggtc ggtgtcctgg aactcgtcga cgacgaggtg ccggaagcgg
     2401 tcctggaagt gctcgcgggc ggcacgcgag gtgcggagga ggtcgcgggc ggcgaggagg
     2461 aggtcgtcga aatcgaggag gccgcgctcg cgcatgcgcg aggcgaggcg cgggaggagc
     2521 ccgccgcgga tcgaggccgc gacgcgcgcg aggagcctct cgtcggaaag cgacgcgagg
     2581 cgatccggca gctccttcca ttcgtcccgg aaggcgtcgg cgagctcttt gagccggccc
     2641 gcgtagacgc cgcgcttgcc gacgcggaag tcgaccttcg tctcgagccg ggcggaggcg
     2701 aggccgtcga ggtcgagagg gaggagcgca cggacctcct cgaccgcgga gcggaggagg
     2761 agcgccttcg cgtcggtccg gaggtccgaa gggaactcct cgacggcgcg gtcgccggcg
     2821 tcggcgaggc tcgcgagctc gaggcgaaag tacggaagag ggtcgtcggg gatcgtcccg
     2881 gcaaggacga gcgtccgctc ggagcggatc gtccgggcga ggtcgcggag ggtgtcgggc
     2941 gagaggccgc gccggagggc gtcggtcacg aagccgccgg cccgggcctc ctcgtcgacc
     3001 cacgcggcga agacgtcgtc gaagagctcc tccgcgacgc cgtcctcggg gacgacgaag
     3061 tcggggtcga cgcgcgcctc cacgggccgc tcgcgcagga gccgggcgca gaacgagtgg
     3121 atcgtcgtca cctcggcgcc gtcgaggtcg cgccgcgcgc ggagggcccg ctcgcgccgg
     3181 gcggcgtccg tggccgcgcg cgcctcctcg tcgaggcgcg tccggaggcg ggccttcatc
     3241 tcgcccgcgg ccttctccgt gaacgtcagg gcggcgacgg tcgagagcgg ctcgccctcg
     3301 tcgaggacga ggcagacgat ccggtcgacg agggtcttcg tcttgccggt gccggcggag
     3361 gcctcgacga ggagcgaggt ccccacgtcg gtccgcgcgc gctcgcgggc ggcgtcgtcg
     3421 acgagcggcg ccgtcacggg atctcctcga gcgcgaagag cggcgcggca gggtcggcga
     3481 gccgctcggc ctcgcgcttg gtcgcgtaga cgcgcgcgtg ccccggcccg cagacggccg
     3541 cgacgtcgca gaacgagcag gggctcccgc ggctggagag cgactccgtc cgcgggagga
     3601 ggtccccctt ccggaccgtc gcgtcgagga ggcccaccgc gcggtggaac gaggcgacgg
     3661 cctcgccggt ctccgcggcg tcgaacgcga cgggaagggc ggttggttcg gcccccttcc
     3721 ggtccgcggt gacgaagagg tactccgagg agacgctccc ggcgccgagc gcccgcgcgg
     3781 cgagcgcgta ggccgcgagc tggaccttct cgccgccgac gatccgccag cccttgcggt
     3841 tcttcgcgcc gaacgggtcg ggccggctga acttgtagtc gacgacgcgg gcggtctcgc
     3901 ctcgccggtc gaggcggtcg atccggccgg tcagggggac gcggagcggg tcctcgcccg
     3961 ccgcgggctc ggcccccctt cccgggccga agccgatctc tcctcctgcc agcgggagcg
     4021 ggtcggggcg agagctctcg agctccccga ccgcccggag gctcgccgcc agctgctccg
     4081 tcgcgacctc gaccaggacg ggcgggagat cgagctccca gtcgagcagg aagcgcgtca
     4141 cggcgcgggc tgcgagagcg tccgcctgcg agaggaggag gtccccgagc gggacttccc
     4201 gttcgagcgc gctcttcgcc gcggcttcga gcgcggcgtg ggcgagctcg cccgtcgtcc
     4261 ggccgtcgag ggcgagctca gcgggcgcct cctccggcgc ggagaggccg agagcgtccg
     4321 tcaggaacgc gcggtaggca caggaagcga gccgctcgag acggctcggg gagaggacgc
     4381 gccctcctcc gaggccgagg cgaagcgggg cgcgcgagag acgcccctcg tactcggtgt
     4441 accgcgcctc ggcgcgcgcc cggccgcgcc ggagcgctcg gcggagcgtc tcgaggccgg
     4501 gcgggagggc cgatcggagc ttcggcgcgc gagcgagggc gcgccggagc gcgtcgtccg
     4561 cgtcgagggg aggctctgcg gtcgcgggcg cgccgaggcg gatccagcgg agcgagaggg
     4621 gggccgcgag ctcgggcttc gcgagctcgg cctcggagaa cgagcgcccg gcgcgctctt
     4681 cgaggtggtc gagcaggaac ggcgagagga ggcgcggccg gtcggtcgcg gcctcgcgcc
     4741 gggcggcgag gaggacgagc cggcgccgcg cggcgctccg cgcgaggtgg aagaggaagc
     4801 gctcctcgcg ggcgtggcgg ggaccggtgc gcggcgcgat cggccggcgc gtggcccggg
     4861 agagcgcggc ccgctcgtcg tcgaagagga gcgggtcggg ccgccccgcg cgcgggaacg
     4921 actgctcgac gaggccgggg acgaggacga cgtcgaagag gaggccgcgc gccgagacgg
     4981 aggagaggag cgagaggccg tcgcgctcga agcgcccctg ccggacgggc gtctcctcga
     5041 gcgaatcggc gaggagcggc aggacgtcga tcgccgcgac ggggacgccc ggctcgaccg
     5101 cctcgagctc gccgaggcgt gtcgccgcgc ccgagagctc gccggcgccg gggaggcgac
     5161 cgaagagcgc gtcgaacgac gccgcgagcc gctcactcca ggtcctccac ggggcgggcg
     5221 ccgggtcggg gaggacgggc gcgaggagag cgacgacctc gcggagcgcc gcgaggtccc
     5281 gcctcacgcg catctctctc cgcgtgaccc gcccctccgc ctcggccgcc tcctcggggg
     5341 gacggcgcgc gaggcgtgcc tcgatccgcg ggacgatccc ggcccagccg tctcggaagc
     5401 ggatctcgag gagagcccgc tccacgcggg cgcggacccc ggggggaagc agggcgaggc
     5461 cttcgaggac gtcgacgagc gcgacgaggt cgtcgggcgc gaaacctccg gccgggagcg
     5521 cgaggagtcc gagcgcggcg cagccgagcg gcgtgcggga gaagccggcg ccggccggcc
     5581 ggaagaaggg gatcctccgg gcgctgagct cggcgacgag ggccggctcc tgccgcgccg
     5641 ggtcgcggac gaggacggcg atccggtgga gggggacgcc gtcgtcgacg gcgcagagga
     5701 cctcgcggac gacctcccgc gcctcggaga gctcggaggg agcgacgacg acgcagactt
     5761 cgggcggcgc gggagcggga cccgcgtcgc gaggagggac gccgagcagc ccttggaaga
     5821 gcgcggcccg gacgcgcggg gcgaccgcat ccgcggcctc ggcgccgacc tcgggctccc
     5881 cgtcgaccgg gacgaacgcg acgacgggcc gctcggcggc gacggccgcg aggagcgcct
     5941 cgcggacgcc gccgaggtcg tagaggccgt agacgaggag cggatcgggg cgcgggggca
     6001 ccttcaccgc ggccgcgcgc gcgaggaggg cgtcggcgtc ggcgaaggcg gagcggagct
     6061 cttcgtaccg ggcgaggacg gctccgacgt tgcccaggaa cgcggcgcgg tcgggcgtcc
     6121 tcgcggccgc ggggaggaga gcgcggaggc tcccggcggc gaggccggcg ccgcgcaggt
     6181 cggcggcggt gcgcgcgacg aggcccgcgg tggacgggcg ctctctgagg tcgccgaact
     6241 cggccccctc cggcgtctcg aggagcgcgc ggcgcgacag gagcgcctct ccgacggacg
     6301 ggagcggcgg ccgcgggtcg gcggccggga cccgctcgcg ggcgaggtcg acgaacgtga
     6361 ggaggcggag gttcgcgatg ccgccgaggg tctccgcggc ccggcgccgc atgtagacgc
     6421 cgaggaggtt cgagccgacc aggacgtcga cggggcggag cgggtcgcct gacttccgct
     6481 cgcgcagctc ctcgaggaag cgcgcctcga gcgcggcgaa gccgccggtg cagagcgtgt
     6541 gccgcgggct cccccccatg ccgtggagtg tacgggggcc ggggcgttcg gagcgtcagt
     6601 ccttccagcg gggggagcgg tctccttcga tcccgaggag gtcgaggacg cgctgcgaga
     6661 cgaagccgag caggtcgtcg atcgtcttcg gccgcgtgta gaagccgggg ctcgccggga
     6721 ggatcgccgc ccccgcctgc gcggccttca ccatcgcctc gagcgtcggg agcggcaggg
     6781 gcgcctcccg cgggacgagg acgagcggcc ggcgctcctt caggtggacg taggcggcgc
     6841 gcgtcaggag gttgtcgcag accccgtaag cgaccttcga gagagtcgtc gcgctgcacg
     6901 ggacgacgac catgccgagg gtccggaacg accccgaggc gatcgacgcg ccgacgtccc
     6961 tgttcggatg gacgacgtcg gcgagcgcgg cgaggtcgtc gagcggcgcg ccgagctcgt
     7021 cggcggcgac gcggcgcgcg ccgtccgtcg cgacgaggtg gacctcgagc ccgccaatcc
     7081 ggcgcagcgt ctcgaggagg tccttcgcgt aagggatgcc ggaagcaccg gagatcccga
     7141 cgacgacgcg gcgaggcgga ggatcggcca tcgtgaggaa gtctagtcgg agcggcgcgc
     7201 gtcgacgacg tcgaacgccc aggcgcggag gcccgggaga gaggcggcct cccgctcgac
     7261 ctcgggcggc gggccggtcg cctcgaggag gaagaggccc tgctccggcc tgacgaggag
     7321 gcgccaggga acgtcgagcc gcgcggcgag cgcgcgaagg gcgtcggcgt ccttcccttc
     7381 gacgaagagg acccgggtca cccggcaggc ctccgccgcg cggttagaat cgccggcgga
     7441 agcgcacggg gtccggtggc ccccctggtc ttcaaaacca gcgtgcacgc tccgagaggg
     7501 gcggcgggtg ggttcgactc ccacacgctt ccgccacgat ctcccctcat ttccggatcg
     7561 ggtgaggctc cgagaaaccg cgcctgcgga tctcctcgat cagctccgcg tcggtcttca
     7621 gcggcttctt gtcgccccag cgggggtcgt cgcgatagac cgggacgcat cccggcgtct
     7681 gccggcagct ctccgccgcg tcgtcccatt ccttccgcgc gttgtcccgg cgcggggagc
     7741 gcgtccgctc cggcacctcc aggccgcgct cgacggctcg gtccatcctc ttctgagccg
     7801 cccgcgcggc ggcctcgtcc gccttcccgg tggggcccgc ggcggtgcgg gcgccggcga
     7861 cgggctccgg gtccgcttcc ggcggatcgc cctcgacgct cggcaggtcc tcgaacgaga
     7921 cgcggctcgc cggcgtcccc ttcgccttca tctctgcggc atgctccgag agcgtcttcg
     7981 ggcgagcggc ggggccggac cggccgagag ccggggcggg ccgggccgtg ggggcgggcg
     8041 tcggcgccgg agccgatgcc tgcgcgagca ggaggaggaa gacgatcatc gcgagcctct
     8101 tctcgccgag gattatgccg ggacggctcg ccggacgggg gcgtcggggg tagaaagtgc
     8161 agacctgacc ccggcggtgg tactctcccg cgatggactc gtcggcgagg ggcgggaccg
     8221 ggaagcggat gacgagcgag gagcgcaatc gcctcctcgt ccggatcgtc aacgagctgg
     8281 acgggctcct gaacctcgag tcggtcctcg gacggctcct cgacaccctg cggacggcgg
     8341 tcggctacga cgcggccggg atcttcgtcc tcggccgcga cgaggcgttc ccgggctggg
     8401 ggagctcctg gaggacgatc gccgcgtcgg cccgccgcgg gttcgacgag ccgatgcccg
     8461 acgtcgaccg gctccttgcg ctcggccacg gcctcgtcgg gcacgttctc aggaccggcg
     8521 agccggccgt cgcgcccgac gtcctcgcgg accccaggta cgtgatgggg cgtccctcca
     8581 cgcgctccga gctcgcggtg ccggtctgcg cggggggacg cctgatcggc gccatgaatc
     8641 tcgagagcga cgagccgcgc gcctactccg aggaggacgc cgagccgctc cgcctgttcg
     8701 ccgacgccgc ggcgatcgcg gtggagaagg cggtcctcta ccggctcttc gtccagcggg
     8761 aacgcctcga ggaggagctg aggggcgagg tcgagctgca ggcggcggcc gggagcgcgg
     8821 cgtggaggga cctccccgga tgggacgcgg tcggcttcac ccggccccgc tacgagatcg
     8881 gcggcgacta ctacgatcgg atcccgctcg gcgacgggcg gctcggcctc gtcgtcgccg
     8941 acgtctcggg gaaggggagc ctcgcggcga tgacggtcgc gacgttccgc gcgatcctcc
     9001 gcgcgcaggt cgacggcggc ggcgagccgg tccggaccgc gacggccgcg aaccggctgc
     9061 tccgggaacg ggtccggccg acggagtacg tcacggcggt ctggggcgtc ctcgaccccg
     9121 cccggggcct tctctcgtac acgaactgcg ggcacgtccc cccgctcctc gtccgcgccg
     9181 acgggagcta cacgcgcctg acgaccggcg gaccgcctct cgggctcctc gcgagcgctc
     9241 gctacctgtc gggcgaggtc tcgatcggcc cgggcgacct cctcctcctc ttcaccgacg
     9301 gcgtcgtcga gagcgagggg ccggcggagg cggagtacgg cgcggatcgc ctcgccgagg
     9361 ccgcccgcga cggacgcggc ctcccgagcg cggagctcgt cgaggcgatc gtccgctccg
     9421 tcgcggagtt ccgcggcgcc gacgagccga cggacgactt caccctcctc gtcctcaagc
     9481 ggaccggaga ggccgcctcg ccgccggacg tccgagccac gcattgcact cccgggcgcg
     9541 agcccgggct ggaggtcccg tgagacgact cgcgcatctc gtcctcctcg cgctcgccgg
     9601 cccgctcctc ggccagatgg cgccccccgc ggagccgggc ccgttccggg agacggacct
     9661 cgtcgagctg accgcgctcg acccgacgat ccgcctcgac gtgaagtacg ccacgaacga
     9721 caacttcgtc gggcgcccgg tctaccggga ggcgagggct ttcctccaga ggccggcggc
     9781 ggaggcgctc gtccgggccc accggaagct cggggagaag ggcttcggcc tcgtcgtctt
     9841 cgacggctac cgcccctggg cggtgacgaa ggcgttctgg gacgccacgc ccgacgagaa
     9901 gaaggtcttc gtggccgacc cgaggcgggg ctcgcgccat aaccggggtt gctccgtcga
     9961 cgtggccctg tacgaccggg cgaccgggaa gacggtcgac atgggaggcg cgtacgacga
    10021 gttcggcccg cgctcctacg tcacgtggga aggcggcacg aaggagcagc tcgagaagcg
    10081 cgacctcctc cggggcgcga tggagcgcga gggcttcttc gtctacccgt gggagtggtg
    10141 gcacttcgac ttcaaggact ggaacgcctt cccgatcctg gacgtcccgt tcgagaagct
    10201 cgggaaggcg gcaggccccg cggccgggcc ggcgaaggcg ccctgacctt cgtcgtatcc
    10261 tccgccgcga tggcccacag gatcctcatc tgcgacgacg agggcgacat ccgcgagtcg
    10321 ctccgcgcga tcctcctcga cgaggggttc gacgtcaccg cggtcgcggc ggggccgaac
    10381 gcggtcgccg agtcccccga ccacgacgcc ctcctcctcg acatcaagat gccgggccag
    10441 gacggcctcg agacgctcgc gcagatccgc aagcggggcg tggcgacgcc ggtgatcatg
    10501 atctcggggc acggcgacgt gaagaccgcc atggaggcga tccgcgccgg cgcggacgac
    10561 ttcctcgaga agccgctcgc cacggagcac gtcctgaacg cgctccgacg cgtcctcgac
    10621 gcctctcgcc tgacgcggga gaaccaggag ctccgcagcc gcctcggcct gacgcgcctc
    10681 gtcggcgacg cgcccgtcat gaagaagctc gtcgcggacg tccagagggt cgctccgacg
    10741 ccggcgacga tcctgatcgt cggcccgagc ggcaccggca aggagctcgt ggcgcgctcg
    10801 gtccacgagg gctcggccgt ccgcggcggc cccttcatcc aggtgaactg cgccgcgatc
    10861 cccgagacgc tcatcgaatc ggagctcttc gggcacgaga agggctcctt caccggggcc
    10921 gagcgcaagc aggtggggaa gttcgtcgag gccgacggcg ggacgatctt cctcgacgag
    10981 atcggcgaca tgtccctctc cgcgcaggcg aaggtcctcc gggtcctgca ggagggcgag
    11041 gtcgagccgg tcgggagtcc gcgggtcgtc cgcgtcaagg tgcgggtcat cgcggcgacg
    11101 aacc
//
LOCUS       DAOTEZ010000098        11098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4156_length_11098_cov_6.955266, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000098 DAOTEZ010000000
VERSION     DAOTEZ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11098
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4156_length_11098_cov_6.955266"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1482)
                     /locus_tag="P5164_07015"
     CDS             complement(<1..1482)
                     /locus_tag="P5164_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRY43683.1"
                     /translation="MSKAFRTTVVLVAALCAASPVAADEGMWMPQQIPDLAPRLAQLG
                     FTGDAKMWADLTGFPMNAVISLGGCSASFISPDGLIATNHHCVQGSLQYNSTPEKNLI
                     VDGYLARTREEELSNGPGSRVWVTVAVNDVTDAITGKLAPKLTDRQRYDAIERRIKER
                     TAACEKDGLRCRVASFFEGLKYYEIAQMEISDVRLVYAPPQGIGNYGGETDNWQWPRH
                     TGDFSFYRAYVGKDGKPAAYSKDNVPFRPKHFLKVSPKGASPGELIFVAGYPGRTQRH
                     ETFAEVKERTEWGFPRYVRRNREMIAILEEVTKGSKELAIKAEPRMRGLQNGMKKNEG
                     VLAGLVKGGILARKEEKQKALEAWIAADPVRQKEFGPALAGLAEMQAESEKTREKDAV
                     FGALYMSSPLLSTANQLYRLSLERPKKDLDRDPSFQERNQARIKEGLERMERSYDNRL
                     DRPMLRYVLHEAAALPAGQRIEPLDTLVGFAAGMPKEEAAKKVE"
     gene            complement(1551..2339)
                     /locus_tag="P5164_07020"
     CDS             complement(1551..2339)
                     /locus_tag="P5164_07020"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HRY43684.1"
                     /translation="MDAPPPGPGATRPGLALALPGRRKPYVMAHRGNRTACPENTLAS
                     FRRALEDDADVLETDLWLSADDVFVCIHDATLERTTDGAGPVEARSLDELKTLSAGCG
                     RPEFASERIPTLAEVAALLPPDRGLALELKSDRFLDPDVGRRLGEELETLGVLDRCVA
                     LSFSRARLEACRRGAPRLPTGWITLEESKPLPGTELLGPYWPLLLLNPLYVALAHRRG
                     QAVAPLDPSPGRRLWLWKLLRCDAVLTDDPAETVRQLGRRPPRI"
     gene            2409..4451
                     /locus_tag="P5164_07025"
     CDS             2409..4451
                     /locus_tag="P5164_07025"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRY43685.1"
                     /translation="MSVGGPLPRRIFLAAALALTAGCSREPARPAPPPERPALLLVTL
                     DTTRADALAPEGDAAATPTFAALVARGVRFSQAYATAPTTLPSHASMLSGLYPAGHGL
                     HENGRRLPDHVPLVTERLRELGYATAAFVSGYPLESPFGLARGFDLYDDELGDGRVER
                     SARETTDRALAWLARPEARPRFLWVHYYDPHDPYEPPEPFRSRFPDDPYLGEIAAMDH
                     ELGRLVTAFEAAAGTRGAGIVVAADHGEGRGDHGEMLHGNLLYQGVMRVPLVVAGAGA
                     GTGTRADPVSLRQVRPTLLGLAGEVADGTLLAPGGAPVLAEAMQPYLNYRWQPQVMGV
                     AGRQKLIRSGRLELYDLHVDPAEARDLAGGVAPDRALARAVADYPLPGASAATAPRPA
                     SDEERRRLASLGYLVAGEAPGAVPADAPRAAEMTALFADLDAAALAFARGSYARAAER
                     FERVLARDGGNLMAAVQLAACRSLLGQEKDALAAFERARRIDPRSVEVRHYLGLHHLK
                     HGQVERAGPLLEAVLEAQPDRLAAIEGLAQVRVRQGRLAEAAALLERAVPLSGDPGTR
                     LVEAGLLRMRLGETAPALAALERARGLQGGAFRRDLELGVLSLAAGRLEEARDALDRV
                     PRSHPDAPMALFKRAQVSVLLGEADRKERVRAARASADATTRRLIEGERLFEGLLP"
     gene            4596..>6145
                     /locus_tag="P5164_07030"
     CDS             4596..>6145
                     /locus_tag="P5164_07030"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRY43686.1"
                     /translation="MKWLLRALMVVLIASPAFGQQTGNVVGKVTMDDGSALPGVSVQA
                     TSNVLPQPRNTVTGANGEFRLPLLPPGRYELTFTLQGMATVKRPASVVLRQDTMVNAT
                     LSIAGVTGAATVVATATLLDTESSALKAAVDEQVIKALPVGQEYRDLVKLVPGVQYTE
                     DSVRGPSAGGSGQDNVYSFDGVNVNLPLFGTLSADPSSHDIEQVAVVKGGADATEFNR
                     AAGITVNTVSRSGTNAFRGSLSYQMQPESLIADRRTTSASVFEEDKDWAVANLGGPIL
                     KDRLFFYASYYSPTSTRDNRANLYGEVPNYKSTRDELFGKLTYSPTDSILLNGSYRHS
                     DREESHYGVGGEAYAASTSNGAKAKQKIAIFEGSWIINDKSYATAKFTDFRNPNAGRP
                     DTVLDVVPSMAVGTTLNVGALDTMGFVTIPVPVAGQTAFNDFIAPIIERYGYLQDGVR
                     KGGGRIGAGTQFDYNDFYRQSYQLGYDVSFGRKVTHNLHVGYQWSKDQEDLYRTSNGW
                     GSITIPGGR"
     assembly_gap    6146..6245
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6246..7315
                     /locus_tag="P5164_07035"
     CDS             <6246..7315
                     /locus_tag="P5164_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRY43687.1"
                     /translation="LGIVWAYQGANTVYANYARYIPTAGSLPRAASWARNSAALINVY
                     FDRNGNFIGSTPEASSTGKIFQDDLKPRKTDEFMVGTTKDLGKGWTGRLHGRYRKSDN
                     FWEDTNNDARIRFNPPPGIPRELYVPNLPDINKELGATSYVIAALDGAFTKYYEAGLE
                     AEWRGRNAYVRGSYVWSHYYGNFDQDNSAGSGTGNDSNIFIGSSNIGDGAGRQLWNFK
                     YGNLRGDRRHQLKLYGSYQLPWRSSVGAYAVYQSGQPWEYHSYEPYISQTTSTSDSNR
                     YAEPAGSRRTDAHYQMDLNYTQTIPIGDLFRLEARIDVFNVFDRQTGYNIQDNVHSAN
                     PGTPQTFYDPRRVQLALKLDF"
     gene            7419..8354
                     /locus_tag="P5164_07040"
     CDS             7419..8354
                     /locus_tag="P5164_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HRY43688.1"
                     /translation="MPSLYAGVDGGGTRTRAVVTTSDLLPLGRGASGPTNASVVPVPH
                     LVQSVTEAVDDALEAAGASRADLALLSCGLAGVDASATAGRLVAALETVFGPGRVRVT
                     TDARIALAGALPDPVGGAGAVLIAGTGAICFGRNAHGLEERAGGWGALIGDEGSASEI
                     ARRGLAAVARDVDGRGPRTKMREALDASEGTRSAVERIRRLFRPGAGPTDTAAYFPVV
                     LEAARAGDAIALGILEWAGRELAWTALTVLRKLGIAGEESTVATVGGVFSAGELVLAP
                     LRERLLEGSPRTRLGPPAHVPEIGAIRLALAEGAR"
     gene            8351..9196
                     /locus_tag="P5164_07045"
     CDS             8351..9196
                     /locus_tag="P5164_07045"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA];
                     GO_process: GO:0097173 - N-acetylmuramic acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HRY43689.1"
                     /translation="MTATLALLETIHAADLEAVRAVGNVLPEAARAVEEIAGRLDAGG
                     RWINVGAGTSGRLGVLDASELPPTFGIPLSLVHGRIAGGRAALVDAVEGAEDDEAAGA
                     AEIRAERLGPKDVVLGIAASGATPYVAGALRAAKEAGALAVALVCAPGSRIAALADLA
                     IVVETGPEVLRGSTRMKAGTAQKLVLNMLSTAVFARLGLVWRGEMVAMRPTNAKLRQR
                     AVDILRRTLGIAEEEARALLSETGWELPPALVAARWRVSPFRASEWIAACRGNVARAL
                     EEEPS"
     gene            9193..10107
                     /locus_tag="P5164_07050"
     CDS             9193..10107
                     /locus_tag="P5164_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine 6-phosphate deacetylase"
                     /protein_id="HRY43690.1"
                     /translation="MTRAGLVDLHVHGAFGVDLLTADTAGLDRLALGLAARGVEGFVP
                     TLVPVPLAELGPLMARLSAWVRSRAPGDGRGAMPLGIHLEGPFVSPSRSGALHRECLL
                     DGSDARTVDAFFDAVGEVPGRPVVTLAPEIPGGLVLVSRFAARGWLVSLGHTEADVPT
                     LDEALRRGARHMTHFGNAMKALHHRDAGPVGWGLLRDGVTVDVIADLHHLSPEMLALV
                     KRCKGAERFALISDAAPCAGQPDGSYSVWGETLTVCEGAVRNATGGLAGSAALLDECA
                     ARLVACGVATEEEAWIAASRTPRRLLVS"
     gene            complement(10124..>11098)
                     /locus_tag="P5164_07055"
     CDS             complement(10124..>11098)
                     /locus_tag="P5164_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43691.1"
                     /translation="TPTDLVFPFFLFIVGTASVLSLGRRASRGAARATLARHALRRGG
                     TIVLVGWALAWFPFTLERLFRLRLPGVLPRIGVVYALGALLLLAAPAKRRAAFLAAAA
                     ALLVALHTALLFLPGYDLSREGNLQAAIDVALLKGHLWKPAWDPEGVLSTLTALATML
                     TGALAGLLLTSKAPAPRRRAFLAAAGTAGVAGGLAVQALGLPLNKGLWTGSYVLFTSG
                     AASLALLLALVVADARSGTRPLAPLVTFGTNPLLAFVLSGLLAKTLLLVKPTVGGTAV
                     PLHRLLFERVFSRVGDPYLGSHVQALVLLAVVWAILRFCEKRGWFWKV"
BASE COUNT         1521 a         4128 c         3895 g         1454 t
ORIGIN      
        1 ctcgaccttc ttcgcggcct cctccttcgg catccccgcg gcgaagccga cgagggtgtc
       61 gagcggctcg atccgctgac cggccgggag ggccgccgcc tcgtgcagga cgtagcggag
      121 catcggccgg tcgaggcggt tgtcgtagga gcgctccatc cgctcgaggc cttccttgat
      181 ccgcgcctgg ttccgctcct ggaaagacgg gtcgcggtcg aggtccttct tggggcgctc
      241 gagggagagg cggtagagct ggttcgcggt cgaaaggagc ggagagctca tgtagagcgc
      301 gccgaagacg gcgtccttct cacgcgtctt ctcgctctcg gcctgcatct cggcgaggcc
      361 ggcgagggcg gggccgaact ccttctggcg caccgggtcg gcggcgatcc aggcctcgag
      421 ggccttctgc ttctcttcct tgcgggccag gatcccgccc ttgacgagcc cggcgaggac
      481 cccctcgttc ttcttcatcc cgttctggag gccccgcatc cgcggctcgg ccttgatcgc
      541 gagctccttc gaacccttcg tgacctcctc gaggatcgcg atcatctccc ggttgcggcg
      601 gacgtaccgc gggaagcccc actccgtccg ctccttcacc tcggcgaacg tctcgtgccg
      661 ctgcgtccgg ccggggtagc cggcgacgaa gatcagctcg ccggggctcg cccccttcgg
      721 ggagaccttc aggaagtgct tcgggcggaa ggggacgttg tccttcgagt aggcggcggg
      781 cttgccgtcc ttgccgacgt aggcgcggta gaaggagaag tcgcccgtgt ggcggggcca
      841 ctgccagttg tcggtctcgc cgccgtagtt gccgatcccc tgcggcgggg cgtagacgag
      901 gcggacgtcg ctgatctcca tctgggcgat ctcgtagtac ttcagcccct cgaagaagga
      961 ggcgacgcgg cagcggaggc cgtccttctc gcaggccgcc gtccgctcct tgatccggcg
     1021 ctcgatcgcg tcgtagcgct gccggtcggt cagcttcgga gcgagcttcc cggtgatcgc
     1081 gtcggtgacg tcgttcacgg cgaccgtcac ccacacgcgc gagccggggc cgttcgagag
     1141 ctcctcctcg cgcgtccgcg cgaggtagcc gtcgacgatc aggttcttct cgggggtcga
     1201 gttgtactgg agcgacccct ggacgcagtg gtggttcgtc gcgatgaggc cgtcgggcga
     1261 gatgaacgag gcgctgcagc cgccgagcga gatgacggcg ttcatcggga agccggtcag
     1321 gtcggcccac atcttcgcgt cgcccgtgaa gccgagctgg gcgaggcggg gggcgaggtc
     1381 ggggatctgc tggggcatcc acatcccctc gtcggcggcg acgggggacg cggcgcagag
     1441 ggccgccacg aggacgacgg tggtacggaa cgctttgctc aagtggaatc ctccctccgg
     1501 aaaaggccgg acctcgcatt atcccttctg cgggacggcc cgggcctcgt tcagatacgg
     1561 ggcggcctcc tcccgagttg ccggaccgtc tcggccgggt cgtccgtcag gacggcgtcg
     1621 cagcggagga gcttccagag ccagagccgg cggcccgggg acgggtcgag cggcgcgacg
     1681 gcctgcccgc ggcggtgggc gagagcgacg tagagcgggt tcaggaggag gagcggccag
     1741 tacggcccga gcagctcggt ccccgggagc ggcttcgact cctcgagcgt gatccagccc
     1801 gtcgggagac ggggcgcacc gcgccggcag gcttccagcc tcgcccgcga gaacgaaagg
     1861 gcgacgcacc tgtcgaggac gccgagcgtc tcgagctcct ctccgagtcg ccggccgacg
     1921 tcgggatcga ggaaccggtc gctcttcagc tcgagggcga gcccccggtc gggcggcagg
     1981 agcgcggcca cctcggcgag ggtcgggatt cgctcggagg cgaactccgg ccgcccgcag
     2041 ccggcggaga gcgtcttcag ctcgtcaagg ctcctcgcct cgacgggtcc ggccccgtcg
     2101 gtcgtcctct cgagcgtggc gtcgtggatg cagacgaaga cgtcgtcggc cgaaagccag
     2161 aggtcggtct cgaggacgtc ggcgtcgtcc tcgagggcgc ggcggaacga ggcgagggtg
     2221 ttctccgggc aggcggtccg gttcccgcgg tgcgccatca cgtacggctt gcggcgcccg
     2281 gggagcgcga gggcgaggcc gggcctcgtg gcgccgggac cgggaggcgg ggcgtccatc
     2341 cgccggcgat tctggcagct tccgggacgc cgcggtcgcg gctcggggtc ggctagactt
     2401 tcggcctcgt gagcgtcggc ggacctctcc cgcgccggat cttcctcgcg gccgcgctgg
     2461 cgctgacggc cggctgttcc cgcgagcccg cccggccggc gccccctccg gagcgccccg
     2521 cgctcctcct cgtcaccctc gacacgacgc gcgccgacgc cctcgccccc gagggggacg
     2581 ccgccgcgac ccccaccttc gcggccctcg tcgcgcgcgg cgtccgcttc tcccaggcct
     2641 acgcgaccgc cccgacgacc ctcccctccc acgcctcgat gctctccggg ctctacccgg
     2701 cggggcacgg cctccacgag aacgggaggc gcctgccgga ccacgtcccg ctcgtcaccg
     2761 agcggctgcg cgagctcggc tacgccacgg ccgcgttcgt ctcgggctat ccgctcgaga
     2821 gcccgttcgg cctcgcccgc gggttcgacc tctacgacga tgagctcggc gacggacggg
     2881 tcgagcgctc cgcccgcgag acgaccgacc gcgcccttgc ctggctcgcg cgacccgagg
     2941 cccgcccgcg attcctctgg gtccactact acgacccgca cgacccctac gagccgcccg
     3001 agccgttccg cagccgcttt ccggacgacc cgtacctcgg cgagatcgcc gcgatggacc
     3061 acgagctggg gcgcctcgtc accgcgttcg aggcggcggc cgggacgcgc ggggcgggca
     3121 tcgtcgtcgc cgccgaccac ggcgaggggc gcggcgacca cggcgagatg ctgcacggga
     3181 acctcctcta ccagggcgtg atgcgcgtgc cgctcgtcgt cgccggcgcc ggggccggga
     3241 cggggacgcg cgctgatccc gtcagcctcc gccaggtccg gccgacgctc ctcggcctcg
     3301 ccggcgaggt agccgacggc acgctcctcg cgcccggcgg cgctccggtc ctcgccgagg
     3361 cgatgcagcc gtacctcaac taccgctggc agccgcaggt gatgggcgtc gcggggcggc
     3421 agaagctgat ccgctccggc cgtctcgagc tctacgacct gcacgtcgac ccggcggagg
     3481 cgcgggacct cgccggaggc gtcgccccgg accgcgccct cgcccgggcc gtcgcggatt
     3541 acccgctgcc gggcgcgtcc gccgccaccg ccccgagacc ggccagcgac gaagagcggc
     3601 ggcgcctcgc cagcctcggc tacctcgtcg cgggggaggc ccccggcgcc gtccccgcgg
     3661 acgcgccgcg cgcagcggag atgacggccc tcttcgccga cctcgacgcc gccgccctcg
     3721 ccttcgcccg cgggagctac gcccgcgccg ccgagcgctt cgagcgcgtc ctcgcgcgcg
     3781 acggcgggaa cctgatggcc gccgtccagc tcgccgcctg ccgctcgctc ctcggccagg
     3841 agaaggacgc gctcgccgcc ttcgagcggg cgcggcgaat cgacccgcgc tcggtcgagg
     3901 tccggcacta cctcggcctg caccatctga agcacgggca ggtcgagcgc gccgggcctc
     3961 tcctcgaggc ggtcctcgag gcccagcccg accggctcgc cgcgatcgag gggctcgccc
     4021 aggtccgggt tcgccagggg cggctcgcgg aggcggcggc gctcctcgag cgggccgtcc
     4081 cgctctccgg cgacccgggg acgcgcctcg tcgaggccgg cctcctccgg atgcggctcg
     4141 gcgagaccgc gcccgccctc gccgccctcg agagggcgcg aggcctgcag ggaggcgcgt
     4201 tccgcaggga cctggagctc ggcgtcctct ccctcgcggc cggccggctc gaggaggccc
     4261 gcgatgccct cgatcgcgtc ccccgcagcc accccgacgc tccgatggcg ctcttcaaga
     4321 gggcccaggt gagcgtcctc ctcggcgaag ccgaccggaa ggagagggtc cgcgccgccc
     4381 gcgcgagcgc cgacgcgacg acgcgacggt tgatcgaagg ggagcggctc ttcgaaggcc
     4441 tcctcccctg acgggccccc cggacgggct cgaatccgcg cctcgcgccg gcacggcctc
     4501 ggctacacta ggctgcagta ttccgtccgg cgtttccctg ggtaagacac tcgccgcgcc
     4561 attcggcgtg gctacctgat agggaggaga ttcgaatgaa gtggctcctg agggcactga
     4621 tggtggtgct gatcgcttcg cctgcgttcg gccagcagac gggcaacgtc gtcggcaagg
     4681 tcacgatgga cgacggctcc gccctgcccg gggtctccgt ccaggcgacc tcgaacgtcc
     4741 tgccgcagcc ccggaacacc gtcaccggcg cgaacggcga gttccgcctc ccgctcctcc
     4801 cgcccggcag gtacgagctg acgttcaccc tccaggggat ggccaccgtg aagcgcccgg
     4861 cgagcgtcgt cctccgccag gacacgatgg tcaacgcgac gctctcgatc gccggggtca
     4921 ccggagcggc gaccgtcgtc gcgacggcca cgctcctcga caccgagtcg tccgcgctga
     4981 aggccgccgt cgacgagcag gtcatcaagg ccctgccggt cggccaggag taccgcgacc
     5041 tcgtcaagct cgtccccggg gtccagtaca ccgaggactc cgtccgcggc ccgagcgcgg
     5101 gcggcagcgg ccaggacaac gtctacagct tcgacggcgt gaacgtgaac ctgccgctct
     5161 tcggcaccct ctcggccgac ccctcctcgc acgacatcga gcaggtcgcg gtcgtcaagg
     5221 gaggcgccga cgccaccgag ttcaaccgcg cggccggcat cacggtgaac accgtcagcc
     5281 gctcggggac gaacgcgttc cgcggcagcc tctcgtacca gatgcagccg gagagcctga
     5341 tcgccgaccg gaggacgacc agcgcctccg tcttcgagga ggacaaggac tgggcggtcg
     5401 ccaacctcgg cggcccgatc ctgaaggacc gcctcttctt ctacgcctcc tactacagcc
     5461 cgacgagcac gcgcgacaac cgcgcgaacc tctacggcga ggtcccgaac tacaagagca
     5521 cgcgcgacga gctcttcggc aagctcacct actccccgac cgactcgatc ctcctcaacg
     5581 gctcctaccg gcactcggac cgcgaggaga gccactacgg ggtcggcgga gaggcctacg
     5641 ccgcctcgac gagcaacggc gcgaaggcga agcagaagat cgcgatcttc gagggctcct
     5701 ggatcatcaa cgacaagagc tacgcgacgg cgaagttcac agacttccgc aacccgaacg
     5761 cggggaggcc cgacacggtc ctcgacgtcg tcccgtcgat ggcggtgggc acgaccctga
     5821 acgtcggcgc cctcgacacg atgggcttcg tcacgatccc cgtcccggtg gcggggcaga
     5881 ccgccttcaa cgacttcatc gccccgatca tcgagaggta cggctacctg caggacggcg
     5941 ttcgcaaggg aggcggccgg atcggcgccg ggacgcagtt cgactacaac gacttctacc
     6001 gccagagcta ccagctcggc tacgacgtct ccttcggccg gaaggtgacc cacaacctgc
     6061 acgtcggcta ccagtggtcg aaggaccagg aggacctcta ccggacctcc aacggctggg
     6121 gctcgatcac gatccccggc gggcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnggctc ggcatcgtct gggcctacca gggggcgaac accgtctacg cgaactacgc
     6301 ccgctacatc ccgacggccg gctcgctccc gcgcgccgcg tcgtgggccc ggaactcggc
     6361 cgcgctcatc aacgtctact tcgaccggaa cggcaacttc atcggctcga cgcccgaagc
     6421 ctcctccacg gggaagatct tccaggacga cctcaagccc cggaagaccg acgagttcat
     6481 ggtcgggacg acgaaggacc tcgggaaggg gtggaccggc cgactgcacg gccgttaccg
     6541 gaagtccgac aacttctggg aggacacgaa caacgacgcg cggatccgct tcaatccccc
     6601 tccggggatc ccgcgtgagc tctacgtccc gaacctcccg gacatcaaca aggagctcgg
     6661 cgcgacgtcg tacgtcatcg ccgcgctcga cggcgcgttc acgaagtact acgaagccgg
     6721 actcgaggcc gagtggcgcg gccgcaacgc ctacgtccgc ggctcctacg tctggagcca
     6781 ctactacggc aacttcgacc aggacaactc cgccggctcc ggcacgggca acgacagcaa
     6841 catcttcatc ggctcgtcga acatcggcga cggcgccggc cgacagctct ggaacttcaa
     6901 gtacggcaac ctccgcggcg accgccgcca ccagctgaag ctctacggct cctaccagct
     6961 cccctggcgg agctccgtcg gcgcctacgc ggtctaccag tcgggccagc cgtgggagta
     7021 ccacagctac gagccgtaca tctcgcagac gacgagcacg agcgactcga accgctacgc
     7081 ggagccggcc ggctcccgcc ggaccgacgc gcactaccag atggacctga actacacgca
     7141 gacgatcccg atcggggacc tcttccggct cgaggcccgc atcgacgtct tcaacgtctt
     7201 cgaccgtcag accggctaca acatccagga caacgtccac tccgccaacc ccggtacgcc
     7261 gcagacgttc tacgacccgc gccgggtgca gctcgccctc aagctcgatt tctagcctcc
     7321 gccaggaacc ggaatccgaa gggcccgcgg agcgatccgc gggcctttcc ctttccggaa
     7381 cccggccccg ggcgggcttc gggcggtacg attccctcat gcccagcctc tacgccggcg
     7441 tcgacggcgg cgggacgcgc acgcgcgccg tcgtgacgac ctcggacctc ctcccgctcg
     7501 ggcgcggcgc ctccggcccc acgaacgcct cggtcgtccc cgtcccgcac ctcgtccagt
     7561 ccgtcacgga ggcggtcgac gacgccctcg aggccgccgg cgcctcgcgc gccgacctcg
     7621 cgctcctctc ctgcgggctc gccggcgtcg atgcctccgc caccgccgga cggctcgtcg
     7681 ccgccctcga gaccgtcttc ggaccggggc gcgtccgcgt gacgaccgac gcgcgcatcg
     7741 ccctcgccgg ggccctcccc gatcccgtcg gcggggccgg ggccgtcctg atcgccggca
     7801 ccggcgcgat ctgcttcggg cggaacgccc acggcctcga ggagcgcgcc gggggctggg
     7861 gagccctgat cggcgacgag ggctccgcct cggagatcgc ccggcgcggc ctcgccgccg
     7921 tggcgcgcga cgtcgacggc cgcggccccc gaacgaagat gagggaggcg ctcgacgcct
     7981 cggaggggac ccgctccgcc gtcgagagga tccggcgcct cttccgtccc ggcgcggggc
     8041 ccaccgacac cgccgcctac tttcccgtcg tcctcgaggc ggcgcgcgcg ggcgacgcca
     8101 tcgcgctcgg gatcctcgaa tgggcgggcc gcgagctcgc ctggaccgcg ctcaccgtgc
     8161 tccggaagct cggaatcgcc ggcgaggaat cgaccgtcgc gacggtcggc ggcgtcttct
     8221 cggcgggcga gctcgtcctc gcccccctcc gcgagcggct cctcgagggc tccccgcgga
     8281 cgcggctcgg ccctccggcg cacgtgcccg agatcggcgc catccgcctc gcgctcgccg
     8341 agggggcgcg gtgacggcga cgctcgcgct cctcgagacg atccacgcgg ccgacctgga
     8401 ggccgttcgc gccgtcggga acgtcctccc cgaggcggcg cgcgcggtgg aggagatcgc
     8461 cggacggctg gatgcgggcg ggcgatggat caacgtcggg gccgggacga gcggccggct
     8521 cggcgtcctc gacgcctccg agctcccccc gacgttcggg atccctctct ccctcgtcca
     8581 cggccggatc gccggcgggc gggcggccct cgtcgacgcc gtcgaggggg cggaggacga
     8641 cgaggcggcc ggggcggcgg agatccgggc ggagcggctc ggtccgaaag acgtcgtcct
     8701 cgggattgcg gcgagcggcg cgacacccta cgtcgccggc gcgctccgtg ccgcgaagga
     8761 ggcgggcgcg ctcgccgtcg ccctggtctg cgcccccggc tcgcggatcg ccgcgctcgc
     8821 cgacctcgcg atcgtcgtcg agaccgggcc ggaggtcctc cgcgggtcga cgcggatgaa
     8881 ggcggggacg gcccagaagc tcgtcctgaa catgctctcc accgccgtct tcgcgcggct
     8941 cggcctcgtc tggcgcgggg agatggtggc gatgcgaccg acgaacgcga agctgaggca
     9001 gcgggcggtc gacatcctcc gccgaacgct cggcattgcc gaggaggagg cgcgcgctct
     9061 cctctcggag accggctggg agctcccgcc ggcgctcgtc gccgcgcgct ggcgcgtctc
     9121 cccgtttcgc gcctcggagt ggatcgccgc ctgccggggg aacgtcgccc gcgccctcga
     9181 ggaggagccg tcgtgacccg cgccggcctc gtcgacctcc acgttcacgg cgcgttcggc
     9241 gtcgacctcc tgaccgcgga cacggcgggg ctcgaccggc tcgcgctcgg cctcgcggcg
     9301 cgcggcgtcg agggcttcgt cccgacgctc gtccccgtcc ctctcgccga gctcggaccg
     9361 ctgatggccc gcctctccgc gtgggtccgc tcccgagcgc ccggcgacgg gcgcggggcg
     9421 atgccgctcg gcatccacct cgaggggccg ttcgtctcgc cgagccgctc gggcgccctt
     9481 caccgcgaat gcctgctcga cgggagcgac gcccggacgg tcgacgcctt cttcgacgcc
     9541 gtcggcgagg tccccggccg gcccgtcgtc acgctggcgc ccgagatccc gggagggctc
     9601 gtgctcgtct cccgcttcgc cgcgcgcggc tggctcgtct ccctcggcca caccgaggcc
     9661 gacgtcccga cgctcgacga ggcgctccgg cgcggcgcgc ggcacatgac ccacttcggg
     9721 aacgcgatga aggcgctcca ccaccgcgac gccggtccgg tcggatgggg gctcctgcgg
     9781 gacggggtga cggtcgacgt gatcgccgac ctccatcacc tctcccccga gatgctcgcg
     9841 ctcgtgaagc gctgcaaggg agcggagcgc ttcgcgctga tcagcgacgc ggcaccgtgc
     9901 gccggccagc ccgacgggag ctactccgtc tggggcgaga cgctcaccgt ctgcgaggga
     9961 gcggtccgga acgcgacggg cggcctcgcc ggaagcgcgg cgctcctcga cgagtgcgcc
    10021 gcccgcctcg tcgcctgcgg cgtcgcgacg gaggaggaag cgtggatcgc ggcgtcgcga
    10081 acgccccggc gcctcctcgt ctcctgacgt cccgggcggc ccctcagacc ttccagaacc
    10141 agccgcgctt ctcgcagaag cggaggatcg cccagacgac ggcgaggagg acgagcgcct
    10201 gcacgtgcga tccgaggtac gggtcgccca cgcgcgaaaa gaccctctcg aagaggagcc
    10261 ggtggagcgg caccgccgtc ccgccgaccg tcggcttcac gaggagcagg gtcttcgcga
    10321 ggaggccgga gaggacgaac gcgaggagcg ggttcgtccc gaacgtcacg aggggcgcga
    10381 gcggtcgcgt cccgcttcga gcgtcggcga cgacgagggc gaggaggagc gcgagactgg
    10441 ccgcgcccga cgtgaagagg acgtaggagc cggtccagag ccccttgttg agcggaaggc
    10501 cgagcgcctg cacggccagg ccgcccgcca ccccggcggt gcccgcggcg gcgaggaacg
    10561 cccggcgccg cggggccgga gccttcgacg tcagcagcag ccccgcgagg gcgccggtca
    10621 gcatcgtcgc gagggccgtc agcgtcgaga ggaccccctc cgggtcccac gcgggcttcc
    10681 agagatggcc cttcaggagc gccacgtcga tcgccgcctg gaggttcccc tcgcgtgaca
    10741 ggtcgtagcc gggaaggaag aggagcgcgg tgtggagggc gacgaggagc gcggcggccg
    10801 cagcgaggaa ggcggccctc cgcttcgccg gggcggcgag gaggaggagc gcgccgaggg
    10861 cgtagacgac gccgatccgg gggaggacgc cggggaggcg gaggcggaag agccgttcga
    10921 gcgtgaacgg gaaccaggcg agcgcccatc cgacgaggac gatcgtcccc ccgcggcgga
    10981 gcgcgtgccg cgccagcgtc gcccgcgcgg ccccccgcga ggcgcgccgg ccgagcgaga
    11041 ggaccgaggc cgtcccgacg atgaagagga agaacgggaa gacgaggtcg gtcggcgt
//
LOCUS       DAOTEZ010000099        10934 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4246_length_10934_cov_6.292766, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000099 DAOTEZ010000000
VERSION     DAOTEZ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10934
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4246_length_10934_cov_6.292766"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /gene="pheS"
                     /locus_tag="P5164_07060"
     CDS             complement(<1..1054)
                     /gene="pheS"
                     /locus_tag="P5164_07060"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRY43692.1"
                     /translation="MSSAPFSVEEARGLLDAELADAAGRGGLLPADVHELKVRWLGKK
                     QGVVTALLARLKEVPKEEKAAFGAGVNALKQEVEARLDALEAEATARAAARAEAAKAV
                     DVTLPPRVPPVGRLHPLTVVRRRIEHVFHGLGYEVADGPEIETDWYNFESLNFAPDHP
                     ARDSVDTFFVEGGGTGRDALLLRTHTSPVQMRTMEGRRPPIRVLCPGRVYRKDDIDPR
                     HSPVFHQMEGLVVDEGITFAHLRGTLSAFAKSFFGGRAEVRLAPTFFPFVEPGVDCAV
                     SCVFCETGCRICSYSGWIEIMGAGMVHPNVLANAGIDPERFTGFAFGMGIDRIAMLLH
                     GIPDLRLLFENDVRFLD"
     gene            complement(1071..>1328)
                     /gene="rplT"
                     /locus_tag="P5164_07065"
     CDS             complement(1071..>1328)
                     /gene="rplT"
                     /locus_tag="P5164_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRY43693.1"
                     /translation="ELQVERSLAYATRDRKQKKREFRGLWITRINAAARMNDISYSQL
                     VAGLKKAGSDVNRKVLAEIAFQDPKGFAALVSSAKSALAAA"
     assembly_gap    1329..1428
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1640..2164)
                     /gene="infC"
                     /locus_tag="P5164_07070"
     CDS             complement(1640..2164)
                     /gene="infC"
                     /locus_tag="P5164_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011048340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRY43694.1"
                     /translation="MPPQKNSPINEFILRYPEVRLIAADGEQLGVVTPQDALARAREV
                     ALDVVVIAEQAQPPVCKIMDFGKFTFEKKKKEHESKRKQKVTQVKEVKFRPNIDDHDY
                     DFKVKNMIRFIEEGDKVKATIQFRGREMSRLENGRKVLQRLGADLEGKAHAEGNPEML
                     GNRMHLIFAPPKKH"
     gene            complement(2219..4177)
                     /gene="thrS"
                     /locus_tag="P5164_07075"
     CDS             complement(2219..4177)
                     /gene="thrS"
                     /locus_tag="P5164_07075"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010502129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRY43695.1"
                     /translation="MSANAENAVRLTLPDGSVREVTTGTTGEAVAASIGARLAKDALA
                     VKVGGKVLDLKRPITESGPFEVITPKHPDALEVYRHSTAHLTAHAVKRLFPGVKIGIG
                     PAIENGYYYDFDPGRAFTPEDLERIEAEMRKIVAEDAPVERLEMSKAEAVKVFEEQGD
                     HLKVEIVSGIPEGSLSCYRQADFIDLCRGPHVPSTGKLGVFKLTHAAGAYWKGDEKNP
                     MLQRIYGASFLSQKELDEHLLRMEQARARDHRKLGKELGLYLFHPWAPASPFFLPKGA
                     TVYTLLVDYMRSLYPKYGYTEVVTPQVYDVELFKKSGHYQNYHENMFFTVADEREYGV
                     KPMNCPGHFLMFSQQAFSYRDLPVRYADFGRLHRYERSGVTQGLTRVRTFCQDDAHLF
                     VSLEEMPAEMDRFIDLIDEVYGAFGFTDVKVALATRPEKYMGSLENWDLAEKALASAF
                     ERRGRAYVLNPGDGAFYGPKLDFQVTDAIGRAWQLGTLQVDFSNPERFDLEYTAEDGT
                     KKRPVVLHRAVLGSLERFFGILVEHTGGDFPLWLSPEQARVIPVSDKLLEYARSVAAE
                     LRAAGLRVTVDERNEKLGAKIRHGELEKVPALLVVGEKEREAGAVSLRKRHEGDLGSR
                     KLAEVVSDMKQAVAARAATWGAKAPATK"
     gene            complement(4248..4323)
                     /locus_tag="P5164_07080"
     tRNA            complement(4248..4323)
                     /locus_tag="P5164_07080"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4288..4290),aa:Val,seq:cac)
     gene            complement(4346..4420)
                     /locus_tag="P5164_07085"
     tRNA            complement(4346..4420)
                     /locus_tag="P5164_07085"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4386..4388),aa:Val,seq:gac)
     gene            complement(4560..5228)
                     /gene="rpe"
                     /locus_tag="P5164_07090"
     CDS             complement(4560..5228)
                     /gene="rpe"
                     /locus_tag="P5164_07090"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRY43696.1"
                     /translation="MAKLAPSILSADFGRLAAHAREAIAAGADWLHVDVMDGRFVPNL
                     TVGPLVVEALRPLREETGALLDVHLMIVEPERYLADFAKAGADVLTVHVEACPHLHRT
                     VQQIRELGARAGVTLNPATPLSALEEILPDVDQVLVMSVNPGFGGQSFIPGSTERVRR
                     LRAMLDAIGSKAELEVDGGIKPENAREVVDAGATVLVAGSAVFRGDVAANVGRLREAV
                     APPA"
     gene            complement(5282..5779)
                     /locus_tag="P5164_07095"
     CDS             complement(5282..5779)
                     /locus_tag="P5164_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HRY43697.1"
                     /translation="MSARRERFLVRILNVYPPYLGAGIRVLHRKGDDRTFHVEMRLRP
                     WNRNLFGTHFGGSLYAMCDPWFCWLLVRHLGSAYLVWDKAASIEFLEPGRGRVTATFH
                     VSPETVEEIRAAADRDGKTEPVLTAEVVADDGVVVARVTKTLWVRRKDAPKPPGRKAR
                     GPGSP"
     gene            5860..6834
                     /locus_tag="P5164_07100"
     CDS             5860..6834
                     /locus_tag="P5164_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006519534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY43698.1"
                     /translation="MKLVIQIPAWDEEEQIAAAVRELPRQVPGFTRVEVLVIDDGSTD
                     ATARVAKEAGADHVLRLPIHRGLAVAWRAGLDRALALGADVVVSTDADRQYAAADVAT
                     LVEPILRGEAEIVVGDRGVATKEDFSPGKRLMQRFGSWVVRRASGTEVPDATSGFRAL
                     TREAALRLNVFGRMTYTVETLIQAGHKEMAVASVRVGTNPPIRPSRLFRRTSRYVLVQ
                     AANILRITALYKPLPVFLGLSAVLFLLGTGLFGRYAWILFTDPTPGAHLQSLLLATVF
                     LVASFLAFLTGLLADLISINRKLSEEILLRLRRREAAPAAEDRGDARG"
     gene            6815..7873
                     /locus_tag="P5164_07105"
     CDS             6815..7873
                     /locus_tag="P5164_07105"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HRY43699.1"
                     /translation="MATPAADPRRRSLLRRAALTLLWLALLALVARHVAHAPADVLGR
                     LSQARPWLLAAGGAVYALGFAARALRLNLLLPPASRIPFGRAWAVSGATTFLLQVLPF
                     RGGEVASWALYRRVLGTGWARAGALFVLVKTLDSAAFLLAGLAGAAALALRGGAPVLG
                     GVTAVAVGAGVVLLVLLPRLGEPLAAWASRRFAARPRLARGLAEVAGGLRVAREEPRA
                     YLLAFAGALAFLAGHFAALALLFAALGLSPSLAALAVASLASVLSAAVLPSPAGTFGP
                     MEGGFAAGLALEGVPLALGAAATGAVHLVTTLACGVVGLPLLLRRSAAAEERDGDQAF
                     ADESRREPGETTSWPRRQ"
     gene            complement(8029..9729)
                     /locus_tag="P5164_07110"
     CDS             complement(8029..9729)
                     /locus_tag="P5164_07110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY43700.1"
                     /translation="MDLLSVLLLLIAAWGWGELIGPVPGRELEPRFLRFVLGIVALSH
                     VAVLLGAFSLLRPAIVLCVIYGGAVLGILRSWRTSGQACPVPARTGRPVSIGGLVLMA
                     IPILLALWRGLEKDRGADPYHAFLPKAYLLAGGIRPFPGVAESTYPMGWELISAVAFC
                     HGTGGQPALLSAFAFGLAALGAVLCLKGEEPVGAAVAGVAVATNPLLIRTAGTSHIEC
                     GQALLVAGAVLALLRFRTRGEPGAALAAAALLGLLLGTKETAGPLVVLPAMAICVWPG
                     RKRAAPLSRGVLIGAIALLVVPSAYWTLRRTAATGNPLYPAASDRFPTTAVFLPGAER
                     FREIHPDADGLPLASRMARFVPSLAFDGVAEPVFGIVAALMLVSPTMRRRHGPLLSTA
                     ACAFALSPFVLGGQTRFYAPAMPLYGLAAGAIASAAVPWIRERSAWASTLAVVSILGL
                     LSIRFPLHFLALYDDPSDLLRLPPASYDARARRNLESDPLRETILEATRRAERTRRIL
                     VVSTPRHLEFLSVPFIPPSHFVPDLRPALRRAGIDPVEDLGVAEVLVLGESPGTDGTP
                     "
     gene            complement(9832..10437)
                     /gene="pyrE"
                     /locus_tag="P5164_07115"
     CDS             complement(9832..10437)
                     /gene="pyrE"
                     /locus_tag="P5164_07115"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019851076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRY43701.1"
                     /translation="MNAEEALRHFEETEALLSGHFRLSSGLHSDKYLQCAKVLQWPER
                     AAALGAALGAKLAGLGIDAVVSPAMGGLVIGHEVGRALGRRAVFAERVDGAFTLRRGF
                     GLDAGENVVVVEDVVTTGGSTREVLALVKSLGAVAVACAAVVDRRGAPQGGGTETLEG
                     LPFRSVLRMEVPTWPEGDCPYCRLGQEVVSPGSRRLSSAKA"
     gene            complement(10434..>10934)
                     /locus_tag="P5164_07120"
     CDS             complement(10434..>10934)
                     /locus_tag="P5164_07120"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY43702.1"
                     /translation="HFADLFARTNNYRPKRFTDEALMALAARPWPGNVRELKNHVERL
                     MIMTDGDVVDAIDVEAPGEARAAGPVVVRGGRVVREGDREPEAGADEPPSPAAGGVDD
                     VWSRLLSIRSLQEFQDEAEKLFLVARLAENGGNVTRTAEAVETPRSNLYKKLDRYGLK
                     KREDAT"
BASE COUNT         1533 a         3838 c         3853 g         1610 t
ORIGIN      
        1 ggtcgaggaa ccggacgtcg ttctcgaaga ggaggcgcag gtcggggatg ccgtggagga
       61 gcatggcgat ccggtcgatc cccatcccga acgcgaagcc cgtgaagcgc tccgggtcga
      121 tccccgcgtt cgcgaggacg ttcgggtgga ccatcccggc ccccatgatc tcgatccagc
      181 cggagtacga gcagatccgg caccccgtct cgcagaagac gcagctcacg gcgcagtcga
      241 cgcccggctc gacgaacggg aagaacgtcg gcgcgagacg cacctcggca cggcccccga
      301 agaagctctt cgcaaaggcc gagagggtcc cccgcaggtg cgcgaacgtg atcccctcgt
      361 cgacgacgag cccttccatc tggtggaaga cgggcgagtg gcgcgggtcg atgtcgtcct
      421 tgcggtagac gcgccccggg cagaggacgc ggatcggggg cctgcgcccc tccatcgtcc
      481 gcatctgcac cggcgaggtg tgggtccgga ggaggagcgc gtctcgcccc gtcccgcccc
      541 cctcgacgaa gaacgtgtcg accgagtcgc gcgccgggtg gtccggggcg aagttgagcg
      601 actcgaagtt gtaccagtcg gtctcgatct ccggaccgtc cgcgacctcg tagccgaggc
      661 cgtggaagac gtgctcgatc cggcgccgca cgacggtcag cgggtggagg cgcccgaccg
      721 gcgggacgcg cggcggcagc gtgacgtcga cggccttcgc cgcctccgcg cgtgcggccg
      781 cgcgcgcggt cgcctcggcc tcgagcgcgt cgaggcgcgc ctcgacctcc tgcttgaggg
      841 cgttcacgcc ggcgccgaac gcggccttct cctccttcgg cacctccttc aggcgcgcga
      901 ggagggcggt gacgacgccc tgcttcttcc cgagccagcg gaccttcagc tcgtggacgt
      961 cggccgggag gaggccgccg cgtcccgccg cgtcggcgag ctcagcgtcg aggaggcccc
     1021 gggcctcctc gacgctgaaa ggtgccgagc tcatcgctcc ccgctccgcc tcaggcggcg
     1081 gcgagggcgc tcttggcgga cgagacgagg gccgcgaagc ccttcgggtc ctggaaggcg
     1141 atctcggcga ggaccttgcg gttcacgtcc gagccggcct tcttcagccc cgccaccagc
     1201 tgcgagtagc tgatgtcgtt catccgggcg gcggcgttga tgcgggtgat ccagaggccc
     1261 cggaactcgc gcttcttctg cttgcggtcg cgcgtggcgt aggcgagcga acgctcgacc
     1321 tgcagctcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc cttcttcgtc
     1441 aggatgtgcc gcttcatcgc gttgccgcgc ttgaacttcc cgctcgcggt gatcgtgaag
     1501 cgcttggcgg cgccgcggtg ggtcttcctc ttgtgggtgg ccatgggccg ggtctccttg
     1561 ctccgtgtgg cggttcgaaa gccggcggga ggcgggaccc cgcgtcgcgg gacggtcccg
     1621 gagcccctcc ggcgggacgt cagtgcttct tcggcggcgc gaagatgagg tgcatccggt
     1681 tgccgagcat ctcggggttc ccctccgcgt gggccttccc ctccaggtcc gcgccgaggc
     1741 gctgaaggac ctttcgcccg ttctcgaggc gggacatctc gcggccgcgg aactggatcg
     1801 tggccttgac cttgtcgcct tcctcgatga accggatcat gttcttgacc ttgaagtcgt
     1861 agtcgtggtc gtcgatgttc gggcggaact tgacctcctt gacctgggtc accttctgct
     1921 tccgcttcga ctcgtgctcc ttcttcttct tctcgaaggt gaacttgccg aagtccatga
     1981 tcttgcagac gggaggctgg gcctgctccg cgatgaccac gacgtcgagg gccacctcgc
     2041 gcgccctcgc gagcgcgtcc tgcggggtga cgaccccgag ctgctcgccg tcggcggcga
     2101 tgaggcgcac ctcgggatag cggaggatga actcgttgat cggggagttc ttctgggggg
     2161 ggatccgtgg accgcctcga aagaaaatgg cgcttcctcc ttttgctcag acgactcgtt
     2221 acttcgtggc gggcgccttc gcgccccacg tcgccgcacg cgcggcgacg gcctgcttca
     2281 tatcggagac gacctcggcg agcttgcgcg acccgaggtc cccctcgtgg cgcttccgga
     2341 gcgacaccgc cccggcctcg cgctccttct cgccgacgac gaggagcgcc gggaccttct
     2401 cgagctcgcc gtggcggatc ttggcgccga gcttctcgtt ccgctcgtcc accgtcacgc
     2461 ggaggcccgc cgcgcgcagc tcggcggcga cggagcgcgc gtactcgagg agcttgtcgg
     2521 agaccgggat gacgcgcgcc tgctcgggcg agagccagag cgggaagtcg cctcccgtgt
     2581 gctcgacgag gatcccgaag aaccgctcga gcgagccgag caccgcccgg tggaggacca
     2641 ccggccgctt cttggtcccg tcctcggccg tgtactcgag gtcgaaccgc tccgggttcg
     2701 agaagtcgac ctggagcgtc ccgagctgcc aggcgcggcc gatcgcgtcg gtgacctgga
     2761 agtcgagctt cgggccgtag aacgcgccgt cccccggatt gaggacgtac gcgcgtccgc
     2821 ggcgctcgaa cgccgacgcg agcgccttct cggcgaggtc ccagttctcg agcgacccca
     2881 tgtacttctc ggggcgggtc gccagcgcga ccttcacgtc cgtgaagccg aaagcgccgt
     2941 agacctcgtc gatcaggtcg atgaagcggt ccatctccgc cggcatctcc tcgagcgaga
     3001 cgaagagatg ggcgtcgtcc tggcagaagg tccgcacccg ggtcagcccc tgggtcacgc
     3061 ccgagcgctc gtagcggtgg agcctgccga agtcggcgta ccggaccgga aggtcgcggt
     3121 aagagaacgc ctgctgagag aacatcagga agtggcccgg gcagttcatc ggcttgacgc
     3181 cgtactcccg ctcgtcggcc accgtgaaga acatgttctc gtggtagttc tggtagtgcc
     3241 ccgacttctt gaagagctcg acgtcgtaga cctgcggggt cacgacctcc gtgtacccgt
     3301 acttcgggta gaggctccgc atgtagtcga cgaggagcgt gtagaccgtc gcccccttcg
     3361 ggaggaagaa cggcgaggcc ggcgcccacg ggtggaagag gtagagcccc agctccttgc
     3421 cgagcttgcg gtggtcgcgg gcccgggcct gctccatccg gaggaggtgc tcgtcgagct
     3481 ccttctgcga gaggaacgaa gcaccgtaga tccgctggag catcgggttc ttctcgtcgc
     3541 ccttccagta ggcgccggcg gcgtgcgtca gcttgaagac gccgagcttt ccggtggagg
     3601 ggacgtgcgg cccccggcag aggtcgatga agtcggcctg gcggtagcag gagagcgagc
     3661 cctcggggat gccggagacg atctccacct tcaggtggtc gccctgctcc tcgaagacct
     3721 tcacggcctc ggccttcgac atctccagcc gctcgaccgg ggcgtcctcc gccacgatct
     3781 tccgcatctc ggcctcgatc ctctcgaggt cctcgggggt gaaggcgcgg cccgggtcga
     3841 agtcgtagta gtagccgttc tcgatggccg gaccgatccc gatcttcacg cccgggaaga
     3901 ggcgcttcac ggcgtgagcg gtcagatggg cggtggagtg gcggtagacc tcgagcgcgt
     3961 cggggtgctt cggggtgatc acctcgaagg ggcccgactc ggtgatgggg cgcttcaggt
     4021 cgaggacctt cccgcccacc ttgacggcga gcgcgtcctt cgcgaggcgg gcgccgatgg
     4081 aggccgccac cgcctcgccc gtcgtgcccg tagtcacctc ccggacggaa ccgtcgggaa
     4141 gggtcaggcg gacggcgttt tcggcgttgg cggacatggc gacgacctcc tccatctcgt
     4201 ggtggcgggg attcgagatc ggattctggg aggagggggg agcgggatgg taggcgctag
     4261 cggactcgaa ccgctgaccc cttccgtgtg aaggaagtgc tctaccggct gagctaagcg
     4321 cctgcgaacg cagaggaaag cggaatggta ggcgcgagca ggatcgaact gctgacccct
     4381 accgtgtcaa ggtagtgctc tcccactgag ctacgcgcct acgtgctttc cagggctacc
     4441 ggcaggggac ctcgcaggag gtacttcggc cccaccctcg acccggatct gattctcaaa
     4501 agacccgctc cggcacacgt gccgggaccg cggaggataa ggggaccggc ccgaaggcgt
     4561 caagcggggg gggcgactgc ctcccggagc ctgcccacgt tcgccgcgac gtctccccgg
     4621 aagacggcag agccggcgac gaggaccgtg gctccggcgt ccacgacctc gcgggcgttc
     4681 tccggcttga tgccgccgtc gacctcgagc tcggccttcg agccgatggc gtcgagcatc
     4741 gcgcggaggc ggcggacgcg ctcggtggat ccggggatga acgactgccc cccgaagccg
     4801 gggttcaccg acatgacgag cacctggtcg acgtcaggga ggatctcctc gagcgcggag
     4861 aggggcgtcg cggggttgag cgtgacgccc gccctcgctc cgagctcccg gatctgctgc
     4921 accgtgcggt ggaggtgcgg gcaggcctcg acgtggaccg tcaggacgtc ggcgcccgcc
     4981 ttcgcgaagt cggcgagata gcgctcgggc tccacgatca tcaggtggac gtcgaggagc
     5041 gcgccggtct cctcgcgcag cgggcggagc gcctcgacga cgagcgggcc gaccgtgagg
     5101 ttcgggacga accgcccgtc catgacgtcc acgtggagcc agtcggcgcc ggccgcgatc
     5161 gcctcgcggg cgtgggcggc gagccggccg aagtcggcgg agaggatcga gggggcgagc
     5221 ttcgccatgg accgatcttc tccgagatcg ccctcccggg gaaccgcggc ccgccgcgga
     5281 gtcagggcga gcccggccct cgggccttcc ggcccggcgg cttcggcgcg tccttccgcc
     5341 tcacccagag cgtcttcgtc actcgcgcga cgacgacgcc gtcgtcggcg acgacctccg
     5401 ccgtgaggac cggctccgtc ttgccgtccc tgtcggccgc cgcgcggatc tcctcgaccg
     5461 tctccggcga gacgtgaaac gtcgccgtga cgcgccctcg ccccggctcg aggaactcga
     5521 tcgacgcggc cttgtcccag acgagatagg cgctcccgag gtggcgcacg aggagccagc
     5581 agaaccacgg atcgcacatc gcgtagagcg acccgccgaa gtgcgtgccg aagaggttcc
     5641 ggttccacgg gcgcaggcgc atctcgacgt ggaacgtccg gtcgtctccc ttccgatgca
     5701 ggacgcgaat tcccgcgccg aggtagggcg ggtagacgtt gaggatccgg acgaggaagc
     5761 gctcgcggcg cgccgacatg cgccgaacat agtcgatccg cgtcggaggc ggaacgccgc
     5821 gcccctgccg ccggagggct tcggctatcc tcggatgtcg tgaagctcgt catccagatc
     5881 cccgcgtggg acgaggagga gcagatcgcg gccgcggtcc gcgagctccc ccggcaggtc
     5941 cccggcttca cccgcgtcga ggtgctcgtg atcgacgacg gctcgaccga cgcgacggcg
     6001 cgcgtcgcga aggaggcggg cgccgaccac gtcctccgcc tgccgatcca ccgcgggctc
     6061 gcggtcgcct ggcgagccgg gctcgaccgg gcgctggcgc tcggagccga cgtcgtcgtc
     6121 tcgaccgacg ccgaccggca gtacgcggcg gccgacgtcg cgacgctcgt cgagccgatc
     6181 ctgcgcggcg aggcggagat cgtcgtcggc gaccgcgggg tcgcgacgaa ggaggacttc
     6241 tctcccggga agcggctgat gcagcggttc ggctcgtggg tcgtccggcg tgcctccggg
     6301 accgaggtcc ccgacgccac ctccggcttc cgcgctctga cgcgggaggc ggcgctccgg
     6361 ctgaacgtct tcgggcggat gacctacacc gtggagacgc tgatccaggc cgggcacaag
     6421 gagatggccg tcgcttccgt ccgcgtcggg acgaaccctc ccatccgccc gtcccgcctc
     6481 ttccgccgca cgtcgcgcta cgtcctcgtc caggcggcga acatcctccg gatcaccgcc
     6541 ctctacaagc cgctcccggt cttcctcggc ctctccgcgg tcctcttcct cctcgggacc
     6601 gggctcttcg gccgctacgc gtggatcctc ttcaccgacc cgacgcccgg cgcgcacctc
     6661 cagtcgctgc tccttgcgac tgtcttcctc gtcgcgtcgt tcctcgcgtt cctcaccggc
     6721 ctcctcgccg acctgatctc gatcaacagg aagctgtcgg aggagatcct cctgcgcctg
     6781 cgccggcgag aggcggcgcc ggccgccgag gaccgtggcg acgcccgcgg ctgacccccg
     6841 ccggcggtcc ctcctgcgcc gcgcggcgct cacgctcctc tggctcgccc tcctcgcgct
     6901 cgtggcgcgg cacgtcgccc acgcaccggc ggatgtcctc ggccgcctct cgcaggcgcg
     6961 accgtggctc ctcgcggcgg gcggcgccgt ctacgcgctc ggcttcgcgg cgcgcgccct
     7021 ccgcctgaac ctcctcctcc cgccggcgag ccgcattccg ttcggccgcg cgtgggccgt
     7081 ctcgggcgca acgacgttcc tcctgcaggt cctgccgttc cgcggcggcg aggtggcgag
     7141 ctgggcgctc taccggcgcg tcctcggcac cgggtgggcg cgcgcggggg cgctcttcgt
     7201 cctcgtgaag acgctcgaca gcgcggcgtt cctcctcgcc gggctcgccg gggccgccgc
     7261 gctcgccctc cgcggaggcg ccccggtcct cggcggcgtg acggccgtgg cggtcggcgc
     7321 gggggtcgtc ctcctcgtcc tcctgccgcg tctcggcgaa ccgctcgccg cgtgggcctc
     7381 ccgccgcttc gccgcccgcc cgcgcctcgc gcgcgggctc gccgaggtgg cgggcggcct
     7441 gcgtgtggcg cgggaggagc cgcgcgccta cctcctcgcg ttcgcggggg ctctcgcgtt
     7501 cctcgcgggg cacttcgccg cgctcgccct cctcttcgcg gcgctcggcc tctcgccgtc
     7561 gctcgcagcg ctcgcggtcg cctccctcgc ctcggtcctc tcggcggcgg tcctcccctc
     7621 gcccgccggc acgttcgggc cgatggaggg gggcttcgca gcggggctcg cgctggaggg
     7681 cgttccgctg gcgctcggcg cggccgcgac cggggccgtc cacctcgtga cgacgctcgc
     7741 ctgcggcgtc gtcggcctcc cgctcctcct ccgccggtcc gcggcggccg aggagcggga
     7801 tggcgatcag gccttcgcgg acgagagccg gcgcgagccg ggcgagacga cctcctggcc
     7861 caggcggcag taggggcagt ccccctccgg ccacgtcggg acctccattc ggaggaccgg
     7921 ccggaacggg agcccctcga gcgtctccgt cccgccggcc ggcgaggcgt caagaccgcg
     7981 cagcaccgag cccctccacg gagggatcgt cgactgattc tgcggaatct acggcgttcc
     8041 gtcggtcccg ggggattcgc cgaggacgag gacctcggcg actccgaggt cctcgacagg
     8101 atcgattccg gcgcgacgga gcgcgggacg cagatcgggc acgaagtgag acgggggaat
     8161 gaagggcacg gagagaaact cgaggtgcct cggcgtcgag acgaccagta tccggcgagt
     8221 cctctccgca cggcgagtcg cttcgaggat cgtctccctc agcggatcac tttcgaggtt
     8281 ccttctcgcc cgggcgtcgt acgaggcggg cggcagtctc aggaggtccg acgggtcgtc
     8341 gtagagagcg agaaagtgaa gcggaaaacg gatggaaagg aggccgagga tcgagacgac
     8401 ggcgagagtc gacgcccacg ccgagcgttc tcgtatccat gggacggccg ccgaggcgat
     8461 cgcgccggca gcgaggccgt acagaggcat cgcgggtgcg tagaaccgcg tctgcccccc
     8521 gagcacgaac ggcgacaacg cgaaggcgca cgccgccgtt gacaggagcg ggccgtggcg
     8581 cctgcgcatc gtcggagaaa cgagcatcag ggccgcaacg atcccgaaca cgggctcggc
     8641 cacgccatcg aacgcgaggg atggcacgaa ccgagccatc cggctcgcca ggggaaggcc
     8701 gtccgcgtcg gggtggatct cgcggaaccg ctccgccccc gggaggaaca cggcggtcgt
     8761 cggaaagcgg tcggaggcgg ccggatagag cggattcccg gtggccgctg tcctgcggag
     8821 cgtccagtag gcggacggga cgacgagaag cgcgatcgcg ccgatcagga cgcctctgga
     8881 aaggggggcg gctctcttcc gtcccggcca gacgcagatt gccattgccg gcaggacgac
     8941 gagcggcccg gccgtctcct tcgtcccgag cagcaatcca aggagcgcgg cggcagcgag
     9001 cgccgcaccg ggctcgccac gggtccggaa tcggaggagg gcgaggaccg ctccagccac
     9061 caggagggcc tggccacact cgatgtgaga tgtcccggcc gtccgaatga gcagcgggtt
     9121 cgtcgctacg gccactcccg cgacggcggc accgaccggt tcctcgcctt tgagacagag
     9181 caccgcgcca agagccgcca gtccgaaggc aaacgcggag agaagggccg gctgcccgcc
     9241 tgtcccgtgg cagaacgcca ccgcgctgat caattcccag cccatgggat aggtagattc
     9301 cgcgacaccc gggaacgggc ggattccccc cgcgaggagg taggccttgg gcaggaaggc
     9361 gtggtacgga tccgctccgc gatccttctc cagacccctc cagagcgcca ggaggatcgg
     9421 gatggccatg agcacgaggc cgccgatcga gacgggcctc cccgttcgtg ccggcaccgg
     9481 gcaagcctgg cccgatgtcc tccaagatcg aagaatgccg agtacggccc caccatagat
     9541 cacacagagg acgatcgctg gccggagcaa cgagaaagcc cccagcaaga ccgccacgtg
     9601 agagagcgcg acgatgccga ggacgaagcg caggaaccgc ggctccagct ctctgccggg
     9661 gacgggaccg atcagctcgc cccagcccca tgccgcgatc aggaggagaa gaacggagag
     9721 gaggtccaac gcgtcgagaa tagaacaggc tcgaacccct cgattctcga tgagaccgag
     9781 acctcctgct cggggtgccg gccacgtctt ccccgagccg cgagagcgcg atcaggcctt
     9841 cgcggacgag agccggcgcg agccgggcga gacgacctcc tggcccaggc ggcagtaggg
     9901 gcagtccccc tccggccacg tcgggacctc cattcggagg accgaccgga acgggagtcc
     9961 ttcgagcgtc tccgtcccgc cgccctgcgg ggcgccccgg cggtcgacga ccgccgcgca
    10021 ggcgacggcc accgcgccga ggctcttaac gagcgcgagg acctcgcgcg tggagccgcc
    10081 ggtcgtcacg acgtcctcga cgacgacgac gttctcgccg gcgtccaggc cgaagccgcg
    10141 ccggagcgtg aaggccccgt ccacgcgctc ggcgaagacc gcccgacgcc cgagggcgcg
    10201 gccgacctcg tggccgatga cgagcccgcc catcgcgggc gagacgacgg cgtcgatccc
    10261 gagccccgcg agcttcgcgc cgagggcggc cccgagcgcc gcggcgcgct cgggccactg
    10321 gaggaccttc gcgcactgga ggtacttgtc ggagtggagg cccgaggaga gccggaagtg
    10381 ccccgagagc agggcctcgg tctcctcgaa gtggcggagc gcctcttctg cgttcatgtc
    10441 gcgtcctcgc gcttcttcag cccgtaccgg tcgagcttct tgtagaggtt cgaccgaggc
    10501 gtctcgacgg cctcggccgt ccgggtcacg ttcccgccgt tctcggcgag ccgggcgacg
    10561 aggaagagct tctccgcctc gtcctggaac tcctggaggc tccggatcga gaggaggcgc
    10621 gaccagacgt cgtcgacgcc gccggccgcc ggcgacggcg gctcgtccgc gccggcctcc
    10681 ggctcccggt cgccctcgcg gacgacgcgc ccgccccgga cgacgaccgg ccccgcggcg
    10741 cgggcctcgc ccggcgcctc gacgtcgatc gcgtcgacga cgtcgccgtc ggtcatgatc
    10801 atcagccgct cgacgtggtt cttcagctcc cggacgttcc ccggccaggg gcgcgcggcg
    10861 agcgccatga gcgcctcgtc cgtgaagcgc ttcggccggt agttgttcgt ccgggcgaag
    10921 aggtcggcga agtg
//
LOCUS       DAOTEZ010000100        10927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4253_length_10927_cov_6.515912, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000100 DAOTEZ010000000
VERSION     DAOTEZ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10927
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4253_length_10927_cov_6.515912"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="P5164_07125"
     CDS             complement(<1..838)
                     /locus_tag="P5164_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43703.1"
                     /translation="MNGVEETRRALCAFETDGLEGAPARGEGEARIDDEGVSVGGLRV
                     EFLDVDSFADAGRVLTLGLHPAGTLRISMLARRHETFAKALGEARDAARVRGLLAHGL
                     GAPESFEGALLEPGPARDARLLVWPTHVAVVPAGADPFQVPLGGVTDVRFTEGTWEIV
                     LDAPGGPFHFGRLARRTDAFARAVRAARAAMLERCASASGTRLFSDGRAVAASALGSD
                     FERLLVAWSAPERLEGAREIVRRAGGRERSAIGLAELLDPDEEGLAAKVELPENTAAF
                     LLA"
     gene            complement(852..1484)
                     /locus_tag="P5164_07130"
     CDS             complement(852..1484)
                     /locus_tag="P5164_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRY43704.1"
                     /translation="MATIEFTGNYEDLSTDKGYQFKFFCEKCGNGYMSSFKPSKLGMA
                     TSALQAAGNIFGGLLGRAAHGAYEVQRAVGGPAHDAALKDAVAEIRPLFIHCTRCGQW
                     VCGPACFNKKAGLCEGCAPDLDEEMAAAQAEAAREQVQQKAREVDWMKQRDVATVTGA
                     VCPHCGAKTQGGKFCGDCGKPLATKKKCVTCGTEADGSPKFCPECGGRFS"
     gene            1831..4752
                     /locus_tag="P5164_07135"
     CDS             1831..4752
                     /locus_tag="P5164_07135"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HRY43705.1"
                     /translation="MNRKYGLFLASVFAAAALLVPGDVFARPAEEAGFARPAARDVDL
                     RVREPQGGEAMRAGAVARREPLAMAAEALEEYRDVLGRGPEDGFAFRLEEKDRLGQSH
                     VRMMQTYRGIRVLGGDVVVHVDGDGLLGISGRFVGGLDLPVVAALAPAESAGAAIGWI
                     QREGGANVEVVDVLDPVVWALSGEPVLAVPIRAMWVEGEELRFEDVHVDAATGAFVGS
                     LSKIFTAKSRKVYNMNQACLSTGSELPGTLVVNEGGTSSDSAVMGAYDNTGLAYDYYK
                     NVHGRDSYDGSGSVIVSSVHGQFSTGSSCTKNNAAWIDSPYNQMVFGDGDGSTFSNLA
                     RGIDVTTHEFSHAVCSRTADLAYQNDSGALNEANSDILGRAAAFYGGNGSAATRTDWS
                     VGADVFTPGTSGDALRYMYDPAKDGDSADYYPTRNYASGCSPSSSNDYCGVHTNSGIG
                     NLFFYLLSQGGTHPRGKTTVSVTGLGLSKAEKIWYRALSVYMTTSTTFQGARTATANA
                     ASDLYGGTCTAEWQSVQKAWDAVGVPGTWSCGTTYSIAGSAGTSGATVSAGGVSTTSD
                     ASGNYTLAGLAAGTYSVTASKTGCTFSPTSISVTVGPSATGKSFTATCSTGDTALTSG
                     VTLTGQSVAKSAWKYYYIDVPSGATSLVFATTSATADIDIYTQFNAKPTSSSYSCRPY
                     TSSGNETCTATNPSAGRWWLGVYGYAAGSFSVTATVQTAAATYSIAGNAGTSGATVSA
                     GSKSATSDASGAYTITGLTAGTYSVSATKSGCTFSPASLSVTVGPNATGKSFTASCGS
                     GGQVERLTNGSFESLTASTNSAPDGSWSRTAYSGTSFNTLIANGSAPHGGTDYAYVGV
                     YASSSQSVTSANVVIPSAATSATLTFYVSVVTSETTTSTAYDKLNVQLVNASTGSVLA
                     TLGTLSNLNKTASASTYALKSYSVTSYKGQTVKVRFAATNDSSLTTTFRIDDVSLKSD
                     G"
     gene            complement(4824..5987)
                     /locus_tag="P5164_07140"
     CDS             complement(4824..5987)
                     /locus_tag="P5164_07140"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HRY43706.1"
                     /translation="MAKWERRDVVVRTTATGHELTAPVFTAQGRQERPIAYVQANVHG
                     GELQGNAAILGLFDLLESAPLRGTIVLVPRVNPISANQQVGDYVAGVYDLLTGVNFNR
                     GYLYLTGPSRSASAACYVDVDSFAAAHLSAPIGEIRSGFREALKAALAAIEEEAAAWG
                     ADSRLDFALSIQKMAVDADLVLDLHTGDRAPRYLYAPEGAVAAARAFGLPFVLEVPAR
                     FGGALDEASFVPWQDLSDAFRRLGRDDVPRLVDGFTVELGSMNEFSLEAGREDARRIA
                     SALRYYGVLDGEPDAPLGPITACSAADYRSLHAPVGGLVDLAVRPGTRVKAGDVVARL
                     ADPSRCRSLPPRAGDAVFEVRAPEDGVALLFHAFSSVPKGARLFSMMTKTRPL"
     gene            6244..7191
                     /locus_tag="P5164_07145"
     CDS             6244..7191
                     /locus_tag="P5164_07145"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRY43707.1"
                     /translation="MGTVLQVRLPAGGPDLEASFQAAFQAVAEVEEAASLWKAGTELG
                     RVHARAADGATTVVSAPLGALVAEALRISELTGGAFSPAVGALVAAWDLRGIGRRPSP
                     EERRSAAARSRTGGVRYDPATRELGLDPGVTLDLDGIAKGFALDRAAEALRARGVDDA
                     LLNFGGQILVIGPPAGRPPREAHVASPFDGSVPVLAVRLRDTSLSTSSSSERARIVDG
                     QEVGHLLDPRTGELVLAAGSVTVLASSGALADALSTAWAVEGPAGFRRADPRGPFRCS
                     GAVAFVLPAAEGEPVTVADAPFVRLRPPPRGLKVTVIRP"
     gene            7214..8548
                     /locus_tag="P5164_07150"
     CDS             7214..8548
                     /locus_tag="P5164_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43708.1"
                     /translation="MRPISRALFALPICALPLLAGPARSDEPIPAAPPARTEEAPSNE
                     ELARRIEVLSRELEARRLGDASDPAPPAGATGKWGLGPAASKVYAKSSGLSIGGYGES
                     LYQNFDATREDGARSGKTNEWDYLRAVVYLGWKFDKTFVLNSEIEYEHASTGKGGEVS
                     VEFLTLDAMLREELNLRAGLLLAPVGLVNEMHEPTTYLGARRPDTESRILPSTWRANG
                     VGVFGEAGPVAYKLYLTESLSASSFNAANGIRGGRQNGARATADNLAVTGRVDLVSVP
                     GLLAGVSFFSGESGKDLVADGESFGARTTTFDVHADWRFRGLWLRGVFARVSIDDAAL
                     LNRSLGLTGSRSIGERQEGYYLSAGYEVLSRLAPGSQMALTPFVRWEKTDTQKEVPAG
                     WTRDGANERTAWTVGLDFKPIPHLALKVDWQDYEDAAGTGVPQWNVALAYLF"
     gene            8553..9125
                     /locus_tag="P5164_07155"
     CDS             8553..9125
                     /locus_tag="P5164_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HRY43709.1"
                     /translation="MTRRRPFRVRPAAVLLALAALLPGAAGAAVFETQEQALARAFPP
                     PARVVRSTAYLTTAQVEAARKAAQAPVESAVVTRYAAFSIEGTHLGTAYFDDHVVRTA
                     REVLMIVVAPDGSLRSVDVLAFGEPHDYLPREGWLRRAEGKRSGDGLFVGRALAHVTG
                     ATLTTRAIAAAARRVLALDAQIGPPARPAK"
     gene            9133..9687
                     /locus_tag="P5164_07160"
     CDS             9133..9687
                     /locus_tag="P5164_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43710.1"
                     /translation="MKYMEKGGYQNNPLTRLTLWGTFLFLTGLWASNFGMYFSRMGLS
                     PASVVSYYRGSEETFQPARTFGSMVESSHGHFAMMAMVLLVLTHLYVFVPGPRRLKSF
                     FVIGTFGTTFLSEGTGWLVRFLHPAFAVLKPIAFLASQAFLALMLGLLAAFLLRAGRP
                     AALRPVADEEPEAESPQSAAGGAA"
     gene            complement(9692..10603)
                     /locus_tag="P5164_07165"
     CDS             complement(9692..10603)
                     /locus_tag="P5164_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRY43711.1"
                     /translation="MLLDRLLRPVAGLLLVALAPLALDGCVSVGLTRSAAGDGETGEV
                     AVSVYRKTAERNAGVLVAFPVLSELQKVEGGARTTVARSMAPSWSVPGVPPGRYVLRV
                     ARKIDEHGDVVPLKKPYSREFEVAAGQRVDAAVVLEKVPVGWIVLAAVTVVLLVVLAI
                     ELADEVDLPPPPALPVSDLFLHVALAFPWGSATGPGAVEPGVADVFPAPGSVVAARRV
                     AVSFLMSRPLDPGAIEADAVLAVGSLSGEIPGEVAWQAGDQLLRFTPSRDFRSGEEVT
                     VTLDLGRIASADGRSGSGRASTTFRVP"
BASE COUNT         1583 a         3893 c         3920 g         1531 t
ORIGIN      
        1 cggcgaggag gaaggctgcc gtgttctccg ggagctcaac cttcgccgcg agaccctcct
       61 cgtccgggtc gaggagctcg gcgaggccga tggcggagcg ctcgcgcccg ccggcccgcc
      121 ggacgatctc ccgggctcct tcgaggcgct cgggagcgct ccacgccacg aggagccgct
      181 cgaagtcgct tccgagggcg gacgcggcga cggcgcgccc gtcggagaag agtcgggtcc
      241 ccgaggcgga cgcgcaccgc tccagcatcg ccgcgcgcgc ggcgcggacg gcacgagcga
      301 acgcgtcggt ccggcgggcg aggcggccga agtggaaggg gccgccgggt gcgtcgagga
      361 cgatttccca ggtgccctcg gtgaacctca cgtccgtcac gccgccgagc ggcacctgga
      421 acgggtcggc gccggcgggg acgaccgcaa cgtgcgtcgg ccagacgagg agtcgcgcat
      481 cgcgggccgg gcccggctcg aggagcgcgc cctcgaacga ctcgggagcg ccgaggccgt
      541 gggccaggag gccccggaca cgcgccgcgt cgcgggcctc gccgagcgcc ttcgcgaacg
      601 tctcgtgccg gcgggcgagc atcgagatcc ggagggtccc cgcgggatgg aggccgagcg
      661 tcaggacgcg cccggcgtcg gcgaaggagt cgacgtcgag gaactcgacg cgaaggccgc
      721 cgaccgagac gccctcgtcg tcgatccggg cctcgccctc gccgcgcgcg ggggctccct
      781 cgaggccgtc ggtctcgaac gcacagaggg cgcgccgggt ctcctcgacg ccgttcaagg
      841 cgcgtcccgc gtcaggagaa acggccgccg cactcggggc agaacttcgg gctcccgtcc
      901 gcctcggtgc cgcaggtgac gcacttcttc ttcgtcgcga ggggcttccc gcagtcgccg
      961 cagaactttc cgccctgggt cttcgccccg cagtggggac agacggcgcc ggtcaccgtc
     1021 gcgacgtcgc gctgcttcat ccagtcgacc tcgcgggcct tctgctggac ctgctcgcgt
     1081 gcggcctccg cctgggcggc cgccatctcc tcgtcgaggt cgggggcgca gccctcgcag
     1141 aggccggctt tcttgttgaa gcaggcgggg ccgcagaccc actgcccgca gcgggtgcag
     1201 tgaatgaaca gcgggcggat ctcggcgacg gcgtccttca gggcggcgtc gtgcgcgggg
     1261 ccgccgacgg ctcgctgcac ctcgtaggcg ccgtgggcgg ccctcccgag gaggccgccg
     1321 aagatgttgc cggcggcctg gagagccgag gtcgccatcc cgagcttcga cggcttgaag
     1381 ctcgacatgt agccgttccc gcacttctcg cagaagaact tgaactggta gcccttgtcg
     1441 gtggagaggt cctcgtagtt cccggtgaac tcgatcgtgg ccacgctgcc tccggtctct
     1501 ttgtgagcgg aggattatcg gtcagatccc ggcggcgcga gaacgaggtg cccgcgagac
     1561 gcccgcggtc cagaagctga cccggtgaac gagttagacc ccggcgccgg gtcagggcta
     1621 gacccttctg ctctctggcc aacggggggt ctcccgggag cacgacgggc aagaggaggc
     1681 ttcgagccgc atggagccgc cggttcttcg catctgacga agcgagagga ggagtccgaa
     1741 cccgacgctg gccgcggcac agtcgttgca aacgatcgac ccgaatctca tcgaaacggt
     1801 cttcgaccgt atccgggctg gagggactgc atgaatcgta agtacggtct gttcctggcg
     1861 tcggtcttcg cggcggcggc gctcctggtg ccgggcgacg tgttcgcgcg tcccgccgag
     1921 gaggccggct tcgcacgccc ggcggcgcgc gacgtcgatc tccgcgttcg cgagccgcag
     1981 ggtggcgaag cgatgcgggc gggcgccgtc gcccggcgcg agccgctcgc catggccgcc
     2041 gaggcgctcg aggagtaccg cgacgtcctc ggccgcggcc cggaggacgg cttcgccttc
     2101 cggctcgagg agaaggaccg gctcggccag tcgcacgtga ggatgatgca gacctaccgc
     2161 ggaatccggg tcctcggggg cgacgtcgtc gtccacgtcg acggcgacgg gctcctcggg
     2221 atcagcggcc gcttcgtcgg cggcctggac ctcccggtcg tcgcagcgct ggccccggcc
     2281 gagtcggccg gagccgcgat cggctggatc cagcgcgaag gcggggcgaa cgtcgaggtc
     2341 gtcgacgtcc tcgacccggt cgtctgggcg ctttccggcg agccggtcct cgccgtcccg
     2401 atccgcgcga tgtgggtcga gggggaggag ctccggttcg aggacgttca cgtggacgcc
     2461 gcgaccggcg cgttcgtcgg gtcgctctcg aagatcttca cggcgaagag ccggaaggtc
     2521 tacaacatga accaggcctg cctctcgacc ggcagcgagc tccccggcac gctcgtcgtc
     2581 aacgagggcg ggacctcctc cgactccgcc gtcatgggcg cctacgacaa cacgggcctc
     2641 gcctacgact actacaagaa cgtccacggc cgcgactcgt acgacggctc cggctcggtg
     2701 atcgtctcct cggtccacgg ccagttctcg accggatcga gctgcacgaa gaacaacgcg
     2761 gcctggatcg actcgcccta caaccagatg gtcttcggcg acggcgacgg gagtacgttc
     2821 tcgaacctgg cgcgcggaat cgacgtcacg acccacgagt tctcgcacgc cgtctgctcg
     2881 cggacggccg acctcgccta ccagaacgac tccggcgcgc tgaacgaggc gaactcggac
     2941 atcctcgggc gcgccgcggc cttctacggc gggaacggca gcgccgcgac gcggacggac
     3001 tggtcggtcg gcgccgacgt cttcaccccg ggcacctcgg gcgacgctct ccggtacatg
     3061 tacgaccccg ccaaggacgg cgactcggcc gactactacc cgacccgcaa ctacgcgagc
     3121 ggctgctcgc cgtcgagcag caacgactac tgcggcgtcc acacgaacag cggaatcggc
     3181 aacctcttct tctacctcct ctcgcagggc gggacccacc cgcgcggcaa gacgacggtc
     3241 tccgtcacgg gtctcggcct ctcgaaggcc gagaagatct ggtatcgcgc cctctcggtc
     3301 tacatgacga cctccacgac gttccagggc gctcgcaccg cgacggcgaa cgccgcgtcg
     3361 gacctctacg gcggcacctg cacggcggag tggcagtcgg tccagaaggc gtgggacgcc
     3421 gtgggcgttc cgggaacctg gtcctgcggg acgacctact ccatagcggg cagcgccggg
     3481 acgagcggcg ccaccgtctc ggccggcggc gtctcgacga cctccgacgc gagcggcaac
     3541 tacactctcg ccggcctcgc ggcgggcacc tactccgtga ccgcgtcgaa gacgggctgc
     3601 acgttctcgc ccacctcgat ctccgtcacg gtcggcccga gcgcgaccgg gaagagcttc
     3661 acggccacct gctcgacggg cgacaccgcc ctgacgagcg gcgtcaccct caccggccag
     3721 agcgtcgcga agagcgcctg gaagtactac tacatcgacg tcccgtccgg cgcgacgagc
     3781 ctcgtcttcg cgacgacgag cgccacggcc gacatcgaca tctacacgca gttcaacgcc
     3841 aagccgacct cgtcgtcgta cagctgccgc ccgtacacct cgtcgggcaa cgagacctgc
     3901 accgcgacga acccgagcgc cggccgctgg tggctgggcg tctacggcta cgcggccggg
     3961 agcttctcgg tgaccgcgac cgtccagacg gcggcagcga cctactccat cgccggaaac
     4021 gccgggacga gcggggcgac cgtgagcgcg ggctcgaagt cggccacgtc cgacgcgagc
     4081 ggcgcctaca cgatcacggg cctgaccgcc ggcacctatt ccgtctcggc gacgaagtcc
     4141 ggctgcacgt tctcgccggc gtctctctcc gtgacggtcg gcccgaacgc caccggaaag
     4201 agcttcacgg cgagctgcgg ctccggcggg caggtggagc gcctgacgaa cgggagcttc
     4261 gagtcgctca cggcctcgac gaacagcgcg ccggacggtt cctggtcgcg gacggcctac
     4321 agcgggacga gcttcaacac gctgatcgcg aacggcagcg cgccgcacgg cggcaccgac
     4381 tacgcctacg tcggcgtcta cgcctcctcc tcgcagtcgg tcacgagcgc gaacgtcgtc
     4441 atcccgtctg cggcgacgag cgcgacgctc accttctacg tttccgtcgt cacgagcgag
     4501 acgacgacct ccacggcgta cgacaagctg aacgttcagc tcgtcaacgc ctccaccggg
     4561 tcggtcctcg ccacgctggg cacgctctcg aacctgaaca agaccgccag cgcgtcgacg
     4621 tacgcgttga agagctactc ggtgacgtcg tacaaggggc agaccgtcaa ggtccgcttc
     4681 gccgcgacga acgactcctc gctcacgacg acgttccgga tcgacgacgt cagcctgaag
     4741 tcggacggct gaggccggct ggaggagagg agcccgatcg ggcttcgggg ggaggggagc
     4801 gatcctctcc cccctttttc tggtcagagg gggcgtgtct tcgtcatcat ggagaagagc
     4861 cgggcgccct tcggcacgga cgagaaggcg tggaagagca acgcgacgcc gtcctcgggg
     4921 gcgcggacct cgaaaacggc gtcgccggcg cgcggcggga ggctccggca gcgcgacggg
     4981 tcggcgagcc gggccacgac gtcgcccgcc ttgacgcgcg tgccggggcg gacggcgagg
     5041 tcgacgaggc cgccgacggg ggcgtgcaac gatcggtagt cggccgccga gcaggccgtg
     5101 atcggcccga ggggggcgtc cggctcgccg tctaggactc cgtagtagcg gagggcggac
     5161 gcgatgcggc gcgcgtcctc gcggcccgcc tccagggaga actcgttcat cgagccgagc
     5221 tcgacggtga acccgtccac gagccgcggc acgtcgtcgc ggccgagtcg ccgaaaggcg
     5281 tcggagaggt cctgccaggg aacgaaggag gcctcgtcca gggcgccgcc gaagcgtgcg
     5341 gggacctcga ggacgaacgg aagcccgaag gcccgagcgg cggcgacggc accctccggc
     5401 gcgtagaggt agcgcggagc acggtccccg gtgtggagat cgagaacgag atcggcgtcg
     5461 accgccatct tctggatcga gagcgcgaag tcgagcctgg agtcggcccc ccaggcggcc
     5521 gcctcctcct cgattgcggc gagagccgcc ttgagcgcct ctcggaagcc ggaccggatc
     5581 tccccgatgg gggcggagag gtgcgcggcc gcgaacgaat cgacgtcgac gtagcaggcg
     5641 gccgacgcgg aacgggaggg gccggtgagg tagaggtagc ccctgttgaa gttgaccccg
     5701 gtcaggaggt cgtagacccc cgcgacgtag tcgccgacct gctggttcgc ggagatcggg
     5761 ttgacccgcg ggacgaggac gatcgtgcca cgaaggggcg cggactcgag gaggtcgaac
     5821 aggccgagga tggccgcatt gccctgcagc tcgccgccgt ggacgttcgc ctgaacgtac
     5881 gcgatcggcc tctcctgccg accctgggcc gtgaacacgg gtgcggtcag ctcgtgcccg
     5941 gtcgcggtgg tgcggacgac gacgtcccgg cgctcccatt tcgccatgtg ctctcctccg
     6001 tcgggaagtc tagcgaaccg caaacgggtt ccccccatat gatttgcgtc atattgcgac
     6061 gacatctcga ttgacagggt ggcgggtgcg gtctatcttc gtccggtctt gagatcgtct
     6121 ctcaataccg tcggcgcagt ccggactttc gccctcgcac tggccctgct tgcgatcccg
     6181 acggtgggcg caagggcgga ggccggcacg acagaggccg agtccgtccg ggcgcggtgg
     6241 ctgatgggga ccgtccttca ggtccggctt ccagccggtg gtccggacct ggaggcttcg
     6301 ttccaggccg cgttccaggc cgtcgccgag gtcgaggagg ccgcctcgct ctggaaggcg
     6361 gggacggagc tcgggcgcgt gcacgcgcgc gctgccgacg gggcgacgac cgtcgtctcc
     6421 gccccgctcg gcgccctcgt cgcggaggcg ctgcgcatct ccgagctgac cggtggggcc
     6481 ttctcgcccg ccgtcggggc gctggtggcc gcgtgggacc ttcgcggcat agggagacgg
     6541 ccctcgccgg aggaacggcg gagcgctgcg gcacgcagcc gaaccggcgg cgtccgatac
     6601 gaccccgcaa cgcgcgagct cggtctcgac ccgggcgtca cgctcgacct cgacggcatc
     6661 gccaagggct tcgccctcga ccgggcggcg gaggcgctcc gggcccgtgg cgtcgacgac
     6721 gccctcctga acttcggcgg gcagatcctc gtgatcggtc cgcccgccgg ccgtcccccc
     6781 cgggaggcgc atgtcgcctc gccgttcgac ggctccgttc cagtcctcgc cgtccggctc
     6841 cgggacacgt ccctctcgac ctcgtcgagc tccgagcgcg cgcggatcgt cgacgggcag
     6901 gaggtcgggc acctcctcga tccccgcacg ggagagctcg tcctcgccgc cggctcggtg
     6961 accgttctcg cttcgtccgg cgccctcgca gacgctctct ccacggcgtg ggccgtcgag
     7021 ggacccgccg ggttccgacg cgccgaccca agggggccgt tccggtgctc gggcgcggtc
     7081 gcgttcgtcc ttccggccgc ggagggcgag cccgtgacgg ttgccgacgc tcctttcgtc
     7141 cgactccggc cgccgccgcg cggcctcaag gtcaccgtga tcagaccttg attccacctc
     7201 agaggagaaa tcgatgagac cgatctcgag ggcgctcttc gcgctcccga tctgtgccct
     7261 gccccttctc gcgggcccgg cccgctccga cgagcccatc ccggcggccc cgccggccag
     7321 aaccgaggag gccccctcca acgaggagct cgcccggcgc atcgaggtcc tctctcgcga
     7381 gctcgaggcg cgccgcctcg gcgacgcgtc cgatcccgcc ccgccggcgg gggccacggg
     7441 gaagtggggc ctcggccccg ccgcctcgaa ggtctacgcg aagagctccg gcctctcgat
     7501 cggaggctac ggcgagtcgc tctaccagaa cttcgacgcg acgcgcgagg acggcgcccg
     7561 ctccgggaag acgaacgagt gggactacct ccgcgcggtc gtctacctcg gctggaagtt
     7621 cgacaagacc ttcgtcctga acagcgagat cgagtacgag catgcctcga cggggaaggg
     7681 gggcgaggtc tcggtcgagt tcctgaccct cgacgcgatg ctgcgcgaag agctgaacct
     7741 ccgggccggc ctcctcctcg cgccggtcgg cctcgtgaac gagatgcacg agccgacgac
     7801 ctatctcggc gcccgccgcc ccgacacgga gagccggatc ctgccatcga cctggagggc
     7861 gaacggggtg ggcgtcttcg gcgaggcggg gccggtcgcc tacaagctct atctgacgga
     7921 gagcctttct gcttcgtcgt tcaacgcggc gaacggcatc cgcggagggc ggcagaacgg
     7981 cgcccgggcg accgccgaca acctcgccgt gacggggcgc gtcgacctcg tctccgttcc
     8041 agggctcctc gcgggcgttt cgttcttctc gggcgagtcg gggaaggatc tcgtggccga
     8101 cggggagtcg ttcggcgccc ggacgacgac cttcgacgtc cacgccgact ggcggttccg
     8161 cgggctctgg ctgcgagggg tcttcgcgcg cgtctcgatc gacgacgccg cgctcctgaa
     8221 ccggtcgctc ggcctgacgg ggtcgcgctc gatcggcgag cggcaggagg ggtactacct
     8281 ctccgccggc tacgaggtcc tgtcgcgcct cgctcccggc tcgcagatgg cgctgacgcc
     8341 gttcgtgagg tgggagaaga ccgacacgca gaaggaggtc cctgcgggtt ggacgcggga
     8401 cggcgcgaac gagaggacgg cgtggaccgt ggggctcgac ttcaagccga tcccgcacct
     8461 cgcgctgaag gtggactggc aggactacga ggacgccgcg ggcacggggg tcccgcagtg
     8521 gaatgtcgct ctcgcctatc tcttctgact ccgtgacgcg tcggagaccc ttccgggtcc
     8581 ggcccgccgc cgtgctcctc gcgctcgccg cgctcctccc gggcgcggcg ggcgccgccg
     8641 tcttcgagac gcaggagcag gcgctcgccc gggccttccc gccgccggcg cgcgtcgtgc
     8701 gctccacggc gtacctgacg acggcccagg tggaagcggc gcggaaggcc gcgcaggcgc
     8761 cggtcgagtc ggcggtcgtc acgcgatacg cggcgttcag catcgagggg actcaccttg
     8821 gaaccgccta cttcgacgat cacgtcgtga ggaccgcgcg cgaggtcctg atgatcgtcg
     8881 tcgcgcccga cggcagcctc cggagcgtcg acgtcctcgc cttcggcgag ccccacgact
     8941 accttccgcg ggagggatgg ctccgccggg cggaggggaa gcggtccggc gacgggctct
     9001 tcgtgggacg cgcgctcgcc cacgtcaccg gcgccaccct gacgacccgg gccatcgcgg
     9061 cggccgcgcg gcgcgtcctc gcgctcgacg cgcagatcgg cccgccggca aggccggcga
     9121 agtgaggaaa cgatgaagta catggagaag gggggctacc agaacaaccc gctcacccgt
     9181 ctcacgctct gggggacgtt cctcttcctg acggggctct gggcctccaa cttcgggatg
     9241 tacttctcgc ggatggggct ctcgcccgcg agcgtcgtct cctactaccg cggctcggag
     9301 gagacgttcc agccggcgcg cacgttcgga tcgatggtgg agtcgtccca cgggcacttc
     9361 gcgatgatgg cgatggtcct cctcgtcctg acgcacctct acgtcttcgt ccccggcccg
     9421 cggaggctga agtccttctt cgtgatcggg acgttcggca cgaccttcct ctccgagggg
     9481 acgggctggc tcgtccggtt cctccacccc gcgttcgcgg tcctgaagcc gatcgcgttc
     9541 ctcgcctcgc aggcatttct cgccctgatg ctgggccttc tcgcggcgtt cctccttcgg
     9601 gcggggcgtc cggcggctct tcgtccggtc gcggacgagg agcccgaggc cgaatcgccg
     9661 cagagcgctg cgggcggagc cgcctgacgc gtcaaggaac gcggaacgtc gtcgacgcgc
     9721 gtccgctgcc gctccggccg tccgcggacg cgatccgacc gaggtcgagc gtcaccgtca
     9781 cctcctcccc ggatcggaag tcgcgggagg gggtgaagcg gaggagctgg tcgccggcct
     9841 gccacgccac ctcgccgggg atctcgccgg agagcgagcc gaccgcgagg acggcgtccg
     9901 cttcgatcgc gcccggatcg agcggccgcg acatcaggaa gctgacggcg acgcggcggg
     9961 cggcgacgac cgagccgggt gcggggaaga cgtcggccac gccgggctcg acggccccgg
    10021 gacccgtggc cgagccccac ggaaaggcga gcgcgacgtg gaggaagagg tcggagaccg
    10081 gcagcgccgg cggcggcgga aggtcgacct cgtcggcgag ctcgatggcg aggacgacga
    10141 ggagaacgac cgtgacggcc gccaggacga tccacccgac ggggaccttc tcgaggacga
    10201 cggcggcgtc gacgcgctgt ccggccgcca cttcgaactc gcgggagtac ggcttcttca
    10261 gcggcacaac gtctccgtgc tcgtcgatct tccgggcgac gcggaggacg taacggcccg
    10321 gcggcacccc ggggacggac cacgaagggg ccatcgaacg cgcgacggtc gtgcgcgcgc
    10381 ccccctcgac cttctgcagc tcggagagga cgggaaacgc gacgaggacc ccggcattgc
    10441 gttcggccgt cttccggtag acggaaacgg cgacctcgcc cgtctccccg tcgccggccg
    10501 cgctccgggt gaggccgacc gagacgcagc cgtcgagcgc gagcggagcg agggcgacga
    10561 gcagcagccc ggcgaccgga cgaaggagcc tgtcgaggag catcggccgg aatgctcgtc
    10621 ccgcgcgcgg agccgtgtca acagctccgt gcggtgcgtc ccgtgcggcg cctggcggtc
    10681 cgagcgcgac gcgtgaaggg cgccggggag gccgtcggcc cgccccgggt gccgccgaat
    10741 cgctctccgt cggggcgccc ggagccggag gtccgttccg agggtgcgcc atcggcctcc
    10801 ggcgcgtatc cttgcgccat ggcgtccccc ccgacgagat cggctggcgt gcgcgagcca
    10861 tcgccccgcg gcgcggccgg ccctcacctg gcggacaaca tgggagaggt ccacctcctc
    10921 gaccggc
//
LOCUS       DAOTEZ010000101        10829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4291_length_10829_cov_7.207351, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000101 DAOTEZ010000000
VERSION     DAOTEZ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10829
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4291_length_10829_cov_7.207351"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..1167)
                     /locus_tag="P5164_07170"
     CDS             complement(40..1167)
                     /locus_tag="P5164_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HRY43712.1"
                     /translation="MSATLHQLLKTLVERGGSDLHITTNSPPMVRVHGDIQPLDYPPL
                     TVPETKQLCYSILTDVQKHRFEENLELDLSFGVKGLARFRGNVFMQRGAVSAVFRQIP
                     YEILGFKELGIPSVVENLCNRPRGLVLVTGPTGSGKSTTLATMIDKVNREQPKHIVTI
                     EDPIEFLHTHKKCIVNQRELHADTHSFPNALRAVLREDPDVVLVGEMRDLETMEAALR
                     IAETGHLTFATLHTNSAVQTMNRIIDAFPANQQSQVRAQLSFVLEGILCQTLLPKASG
                     KGRCLAMEILIPTPAIRNLIREDKLHQIYSMMQTGQNKYGMQTFNQALATLVHRKVIS
                     QDLAISTSSLPDELSEMIARGVGVVGQAPPTAAAGAAARRP"
     gene            complement(1421..>2530)
                     /locus_tag="P5164_07175"
     CDS             complement(1421..>2530)
                     /locus_tag="P5164_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRY43713.1"
                     /translation="DIHIEPYEKEYRVRYRIDGVLEEVMNPPSKLKEAIASRIKILAR
                     LDIAEKRLPQDGRIKIKMKLAGKVKELDYRVSVLPTLFGEKIVCRLLDKDNLMLDMTK
                     LGFEKSSLQRFEKAILRPWGMVLVTGPTGSGKTNTLYSALSRINTPDTNIMTAEDPVE
                     FNLPGINQVQMKDSIGLNFAAALRSFLRQDPNIILVGEIRDFETAEIAVKASLTGHLV
                     LSTLHTNDAPSTINRLMNMGIEPYLVATSVVLIAAQRLIRRVCSNCKVPTEVPPQVLI
                     QLGFTPEESKTVQVMKGKGCERCNGTGLKGRVALVEVLEVSEEIKEMILSGASSVEIK
                     RKGVEEGMVTLRGSGLQKIKDGMTTIEEVVRETVL"
     assembly_gap    2531..2630
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2631..3007)
                     /locus_tag="P5164_07180"
     CDS             complement(<2631..3007)
                     /locus_tag="P5164_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HRY43714.1"
                     /translation="MAVKLGELLLKAKLINAEQLAAALKQQKESGVKLGETLVRLGFV
                     TEDDITETLSAQFGVPSINLTHFEIDPNVLKLVPAEVARKYNILPVNKTGATLTIAMA
                     DPTNVFAMDDLKFMTGYNVEPVVA"
     gene            complement(3054..3944)
                     /locus_tag="P5164_07185"
     CDS             complement(3054..3944)
                     /locus_tag="P5164_07185"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRY43715.1"
                     /translation="MPYSKPPSRSSTRGPEPPKRRRFGAVLLALLVGGPLAASVTANV
                     LPNLGRAELPKDQVATVPFRAGETLVYTVSWLKIEGGEMTLATSREWSPDGVPVHRIR
                     LSAVSNDYVSRFYPVDTRYESWVDARDFTPVRFEKRAREGRYSADEVEEFDLGRRVGT
                     WRNAKDRRRVVTLPPRFQDVISSFYFMRTVPLVPGGETRVDLCSRGRLYRLVVAVLGR
                     EKVETELGTFDAIKVQPRMHEGEGSEDQNKGKLFLWFSDDERRLPVMARTILPIGSVT
                     ARLTRVTAESPAAAPPPDAR"
     gene            complement(3898..4905)
                     /gene="waaC"
                     /locus_tag="P5164_07190"
     CDS             complement(3898..4905)
                     /gene="waaC"
                     /locus_tag="P5164_07190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HRY43716.1"
                     /translation="MKILLVRLSSFGDVVFTLPLAKALRGEGDRLAWAVEGPLAGLLE
                     GASYVDETLAATTRAWRKKPFSAETRGELRAFLSLLRAFAPDVVVDAQGLLKSAWVTL
                     AARADRKVGFGFRTATERANALVTRERVDVPPGGHVVDRGLALAERVLGRRGFERTPD
                     VSHLVRRPAPEVDDWLAARRARPFALLQPFSSRATKEWAPAETAAFCRALASRGIDGV
                     LRWGPGERGRAEGIASLAHGAAVVAPPTPPAATARLAARAALFVGADTGPTHLAAAAG
                     TPTVALFGPTDPARFGPVGPRVRILRDESGAYNAGRPGLPGLTHDAVLEASLALLDER
                     T"
     gene            4929..5516
                     /locus_tag="P5164_07195"
     CDS             4929..5516
                     /locus_tag="P5164_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRY43717.1"
                     /translation="MVLDAPVLVLNRSFAALALTDARRGFTLFYKGHVRAVLPDFTTY
                     GWEEWCDIPVQPGDEDVVKTPGLLLKVPRVIQLLSCDRPPRYDVKFSRHNIYLRDGTR
                     CQYCGRRFPTAELSLDHVVPLSRGGVSSWENVVCACLRCNVKKGNRLPDEAGMALRRP
                     PVKPKASPLGLLSPSRIHPAWRSFLDVAYWNVELG"
     gene            complement(5801..6709)
                     /locus_tag="P5164_07200"
     CDS             complement(5801..6709)
                     /locus_tag="P5164_07200"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRY43718.1"
                     /translation="MTRTSKHGALRDRVELGAYLLLRTLFRTLPLSLADGLGRGLGRL
                     YRFVDRRRRRLVARNLALAFPEKPAAEREAISREVFAHFGAIAADLVRSEAAPVEALL
                     ARVEVEGLEHARVAAASGRGVFFATPHLGNWEWANLVTGANGMPVTIVARPLDNPLLD
                     VRLTALRERTGGHVVNKKDAARTILRSLRAGAVVGILGDQRARPPDALLTPFFGRLAW
                     TTTAIARLADRTGALILPVVCLRVGPGRYRLTYSEPIDPAALPPEERGVSRLTARVTA
                     LIESQVRAAPGQWLWLHDRWKGAPEN"
     gene            complement(6706..7731)
                     /gene="lpxK"
                     /locus_tag="P5164_07205"
     CDS             complement(6706..7731)
                     /gene="lpxK"
                     /locus_tag="P5164_07205"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRY43719.1"
                     /translation="MTPLLRPLSTLYGAGVALRLALYRRGLLAVRGAARPVVSVGNVA
                     AGGTGKTPFVRWLAGELLRRGRHPSILTRGYGRGSRGTVVVSDGRGTFATVADSGDEP
                     ALLARALPSVPIVADARRRDAAARAETLGTPVDLHLLDDGFSHVALARDVDVVLLDAT
                     APDAGGALLPAGRLREPLSSLARADLLVVTKTEQSDPGPALEIARRFAPGVPVYRART
                     EVLGVTGRDGLPVEPGDLPPGTAVAVAGIARPEAFGATLASIGIEPVELLSFRDHEPY
                     GPASVGRIERTLEESGATAVVTTEKDAVKLDERLRVPVFRVAVEARPVEPTFVSDLLS
                     LLARRPS"
     gene            complement(7728..8957)
                     /locus_tag="P5164_07210"
     CDS             complement(7728..8957)
                     /locus_tag="P5164_07210"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HRY43720.1"
                     /translation="MTRSDRPSAGGTGLTALLARRLLPEAVPGRVDVWVQGVSVGEVE
                     LAHTLALALRSARPELSLLVTSSTPAGVGLLERRFRDLAPPARLQPFPLDLPVSVRRL
                     LDSATPRLLVLMETELWPVLLRAARRRGLPVLVASGRLSERSVRRLRAARPLFRGGLD
                     ALSAVAARSEEDAERFAAVGVPRERVHVAGDMKFDRPLPAEPAFAASFRELAAGRPVL
                     VAGSIAEEELDLALSLPRLLARGASGTPFLLLAPRRTESWDEAERRAVAAGLRLVRRS
                     RPAPDGPADVFLLDTLGELASAYTLGDVALLGGTFGRRGGHNVLEPLQAGLPVVLGPS
                     VWNIRRVVEAAGAAVVGVPDPAGVAPALARLLDDPAARAAAREAAAALFATHRGATER
                     TVRLALGFLDGRPGAAA"
     gene            9095..9742
                     /locus_tag="P5164_07215"
     CDS             9095..9742
                     /locus_tag="P5164_07215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRY43721.1"
                     /translation="MAIQLVHVRRVQEGTTIEPRDERHLAALAKAGDEDAFAELVRGR
                     TDSVVAFLRRMLGDTEDARDVAQLTFLRVWENLPRYDPSYAFSTWLFRIAGNLAIDAL
                     RSRGTRARTLAESFRVVKGGLLAASPEAPLRLSQEEVRRVFEACASVLSEKQRLVFVL
                     REMEEKESREVAEIVGCRESTVRNHLFQARRLLREELKRRFPEYVPESHRSAEGA"
     gene            9745..10284
                     /locus_tag="P5164_07220"
     CDS             9745..10284
                     /locus_tag="P5164_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43722.1"
                     /translation="MKEPACDRLDDLLARREEDRLTPAEEGFLRGHLASCEVCAAAAL
                     RRDPVLLFARAAAPEPLSADARERFVSGVLAAAATAKAGRRLRRAHTRPVLRLAASLL
                     LAASVAGVWLAREAGNGPAEAPRPDLVAAADEGVRTPPDVVPAVEDLGGDASVVYQFP
                     STRPGEPTVVFVVDRNADI"
     gene            10284..>10829
                     /locus_tag="P5164_07225"
     CDS             10284..>10829
                     /locus_tag="P5164_07225"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HRY43723.1"
                     /translation="MRAPLFPGLLAAALLLAAPAAARTPAPRPTAGPDVVRVFVLKHR
                     KAEDAFLLVRPLLGARGTIVLQTATNALTVRDSVRTVERVAQAVSAFDLPPRAIAVSV
                     SLIRATHAPGPGGPPAPVAERFRGVADRLRSLFNFTEYASLDEVVLQGLEGDPVSWTL
                     GGSFRIDFLLEPGGGRESVRLR"
BASE COUNT         1469 a         3754 c         4029 g         1477 t
ORIGIN      
        1 ccagacgaac tgggccatct caatccctcc cggcgcgggt caggggcgcc gggccgcggc
       61 gcccgcggcg gccgtcggcg gcgcctgtcc cacgacgccg acgccgcggg cgatcatctc
      121 ggagagctcg tccggcaggg acgacgtgga gatcgcgagg tcctgggaga tgaccttgcg
      181 atgcacgagc gtcgcgagcg cctggttgaa ggtctgcatt ccgtacttgt tctgtccggt
      241 ctgcatcatg gagtagatct ggtggagctt gtcctcgcgg atcaggttcc ggatcgccgg
      301 ggtcgggatc aggatctcca tcgcgaggca ccggcccttg ccggaggcct tcgggaggag
      361 cgtctggcag aggatcccct cgaggacgaa cgagagctgc gcccgcacct gcgactgctg
      421 gttcgcgggg aacgcgtcga tgatccggtt catcgtctgg acggcgctgt tcgtgtggag
      481 cgtggcgaac gtgaggtggc cggtctccgc gatccggagg gccgcctcca tcgtctccag
      541 gtcgcgcatc tcgccgacga gcacgacgtc ggggtcctcg cggaggacgg cccggagcgc
      601 gttcgggaac gagtgcgtgt cggcgtgaag ctcccgctgg ttgacgatgc acttcttgtg
      661 ggtgtggagg aactcgatcg ggtcctcgat cgtgacgatg tgcttcggct gctcgcggtt
      721 gaccttgtcg atcatcgtcg cgagcgtcgt cgacttgccc gagccggtcg gccccgtgac
      781 gaggacgagg ccgcgcggcc ggttgcagag gttctcgacg accgacggga tgccgagctc
      841 cttgaagccg aggatctcgt acgggatctg ccggaagacc gccgagaccg cgccccgctg
      901 catgaagacg ttcccgcgga agcgggcgag ccccttgacg ccgaaggaga ggtcgagctc
      961 gaggttctcc tcgaaccggt gcttctggac gtcggtcagg atcgagtagc agagctgctt
     1021 cgtctccggg acggtcaacg gggggtagtc gagcggctgg atatcgccgt gcacccggac
     1081 catcggaggg gagttcgtgg tgatgtggag atccgacccg ccccgctcga cgagagtctt
     1141 cagaagctgg tgcagcgttg ccgacatcga aaccccctgt ccgaggtgct gtcgggaatc
     1201 ataccgcagg cgtttccgca ccccctgccg gaacccgtct cgcccggcgg gaggatcccc
     1261 ggtttcgcgc ctctcgaccc cgcggcgtca gactccggcc gcagaagggg ggcgggggga
     1321 cccggggcgc gaatgaaggc gaggcgccgg tgctctctcc ggtgctctgg gccgcggagg
     1381 gggagcgggg ggacccgggc gcgggtcccc ccggaacatg tcagagcacg gtctcccgga
     1441 cgacctcctc gatcgtcgtc attccgtcct tgatcttctg gagcccggat ccgcggaggg
     1501 tgaccatccc ctcctcgacc cccttccgct tgatctccac ggaggaggcg ccggagagga
     1561 tcatctcctt gatctcctcg gagacctcga ggacctcgac gagcgcgacg cgccccttca
     1621 ggcccgtccc gttgcagcgc tcgcacccct tccccttcat cacctggacg gtcttcgact
     1681 cctcgggagt gaagccgagc tggatgagga cctggggcgg cacctcggtc gggaccttgc
     1741 agttcgagca gacgcgccga atgaggcgct gcgcggcgat caggacgacg gacgtcgcga
     1801 cgaggtacgg ctcgatgccc atgttcatga gccggttgat cgtcgacggc gcgtcgttcg
     1861 tgtggagcgt cgagaggacg aggtggcccg tcagggacgc cttgacggcg atctcggccg
     1921 tctcgaagtc gcggatctcg ccgacgagga tgatgttcgg gtcctgccgc aggaaggaac
     1981 gaagggccgc tgcgaagttc aggccgatcg agtccttcat ctgcacctgg ttgatgccgg
     2041 gcaggttgaa ctcgaccggg tcctcggccg tcatgatgtt cgtgtcgggg gtgttgatcc
     2101 gcgagagggc cgagtagagc gtgttcgtct tcccggagcc ggtcggcccg gtgacgagga
     2161 ccatccccca ggggcggagg atcgccttct cgaaccgctg gaggctcgac ttctcgaagc
     2221 ccagcttcgt catgtcgagc atcaggttgt ccttgtcgag gagccggcag acgatcttct
     2281 cgccgaagag ggtcggcagg acgctgacgc ggtagtcgag ctccttcacc ttcccggcga
     2341 gcttcatctt gatcttgatg cgtccgtcct gcggcaggcg cttctcggcg atgtcgagcc
     2401 gcgcgaggat cttgatgcgc gaggcgatcg cctccttgag cttcgagggg gggttcatca
     2461 cctcctcgag gacgccgtcg atccggtacc ggacgcggta ctccttctcg tagggctcga
     2521 tgtggatgtc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcgacgaccg
     2641 gctcgacgtt gtagccggtc atgaacttca ggtcgtccat cgcgaagacg ttcgtcgggt
     2701 cggccatcgc gatggtgagc gtcgcgccgg tcttgttgac cgggaggatg ttgtacttgc
     2761 gcgcgacctc ggccgggacg agcttcagga cgttcgggtc gatctcgaag tgcgtcaggt
     2821 tgatcgacgg cacgccgaac tgcgccgaca gcgtctcggt gatgtcgtcc tcggtgacga
     2881 agccgagccg cacgagcgtc tcgcccagct tcacccccga ttccttctgc tgcttcaggg
     2941 ccgcggcgag ctgctccgcg ttgatgagct tcgccttcag gagaagttct ccgagcttga
     3001 ccgccatgag ggcaatgata gtcggtcgcc cgcccgccgg caggcccggg gtttcagcgg
     3061 gcgtccggag ggggggcggc ggcgggggac tcggccgtca cgcgggtgag ccgggccgtg
     3121 acggagccga tcgggaggat cgtccgggcc atcaccggga ggcgccgctc gtcgtcgctg
     3181 aaccagagga agagcttccc cttgttctgg tcctcgctcc cctcgccctc gtgcatccgc
     3241 ggctggacct tgatcgcgtc gaaggtgccg agctccgtct cgaccttctc ccggcccagt
     3301 accgccacga cgagccggta gagcctcccg cgcgagcaga ggtcgacgcg ggtctccccg
     3361 ccggggacga gggggacggt cctcatgaag tagaaggagg agatgacgtc ctggaagcgg
     3421 ggcgggagcg tgacgaccct ccggcggtcc ttcgcgttcc gccaggtgcc gacgcgcctt
     3481 ccgaggtcga attcctcgac ctcgtcggcc gagtagcgcc cctcgcgggc ccgcttctcg
     3541 aaccggaccg gggtgaagtc ccgggcgtcg acccaggact cgtagcgggt gtcgacgggg
     3601 tagaagcgag agacgtagtc gttcgacacg gccgaaaggc ggatccggtg gaccgggacc
     3661 ccgtcggggc tccactcccg gctcgtggcg agcgtcatct cgcccccctc gatcttgagc
     3721 cacgagacgg tgtagacgag cgtctcgccg gcgcggaacg gcaccgtcgc gacctggtcc
     3781 ttcgggagct ccgcccggcc gaggttcggg aggacgttcg cggtgacgga ggcggcgagc
     3841 ggccctccga ccaggagcgc gagcaggacc gcgccgaagc ggcgccgttt cggcggctca
     3901 ggtcctctcg tcgaggagcg cgagggaggc ttcgagtacg gcatcgtgcg tcagtccggg
     3961 gaggccgggg cgaccggcat tgtaggctcc gctctcgtct cgcaagatcc ggacccgcgg
     4021 cccgaccggc ccgaagcgtg ccgggtcggt cgggccgaag agggccaccg tcggcgtccc
     4081 ggcggcggcc gcgaggtgcg tcgggccggt gtcggccccg acgaacaggg ccgcccgcgc
     4141 cgcgaggcgc gccgtggcgg cgggcggtgt gggcggagcg acgacggccg cgccgtgggc
     4201 gagggaggcg attccctcgg cgcgccctct ctcgcccggt ccccaccgga ggacgccgtc
     4261 gattccgcgc gaggcgagcg cgcggcagaa cgcggcggtc tcggccgggg cccactcctt
     4321 cgtcgcgcgc gaggagaagg gctgcagcag ggcgaagggg cgcgcgcggc gcgcggcgag
     4381 ccagtcgtcc acttcggggg ccgggcgccg gacgaggtgc gagacgtcgg gcgtcctctc
     4441 gaagcctctc cggccgagga ccctctccgc gagggccagc ccgcggtcga cgacgtggcc
     4501 gcccggcggg acgtcgacgc gctcgcgggt gacgagcgcg ttcgccctct cggtcgccgt
     4561 ccggaagccg aagccgacct tccggtccgc ccgcgcggcg agcgtgaccc aggccgactt
     4621 gaggagtccc tgcgcgtcga cgacgacgtc gggcgcgaac gcgcgcagca acgagaggaa
     4681 cgcacggagc tccccgcgcg tctccgccga gaacggcttc ttcctccacg cccgcgtcgt
     4741 cgcggcgagc gtctcgtcga cgtacgaggc cccctcgagc agaccggcga gcggcccctc
     4801 cacggcccac gcgagacggt cgccttcgcc gcgcagcgcc ttcgcgagag gaagcgtgaa
     4861 gacgacgtcg ccgaacgagg agaggcggac gaggagaatc ttcatgcgcg tttcgctatt
     4921 ctcacgtaat ggtgctcgac gcgccggttc tcgtcctgaa ccggtcgttc gccgccctcg
     4981 ccctcacgga cgcgcgacgc gggttcacgc tcttctacaa ggggcacgtc cgggccgtgc
     5041 tccccgactt cacgacctat ggctgggagg agtggtgcga catcccggtc cagcccggcg
     5101 acgaggacgt cgtgaagact ccgggccttc tcctgaaggt tccgcgggtc atccagctcc
     5161 tctcctgcga ccggcctccg cggtacgacg tcaagttctc ccggcacaac atctacctcc
     5221 gcgacgggac gcggtgccag tactgcggcc ggcgtttccc gaccgcggag ctctcgctcg
     5281 accacgtcgt ccccctctcg agagggggcg tctcctcgtg ggagaacgtc gtctgcgcct
     5341 gcctgcggtg caacgtgaag aaggggaacc ggcttcccga cgaggcgggg atggcgctgc
     5401 gccgtccgcc ggtgaagccg aaggcctccc cgctcgggct cctctcgccg agccggatcc
     5461 acccggcctg gaggagcttc ctcgacgtcg cgtactggaa cgtcgagctc ggctgagccg
     5521 gacgcgggcg cgtcattccg tacagcggca gcgcacggat tcgtgcaccg ggacctcccg
     5581 ggccggcggc cccgggaccc tccggtcgac cgtatctctt tctgcgcacg tcagatgagc
     5641 ctcatgctcc gatcgctcgg ccgccttggc aggaaagttg aaatgacccg aggcgccggg
     5701 gatcgacgct cgcgtcggga gccggcagcg gaggagccta gagagagagc gcaccacagc
     5761 acccgagctt caggaacccc ggcctcaccg ccggggttct tcagttttcg ggggcgccct
     5821 tccagcggtc gtggagccag agccactggc cgggggcggc gcggacctgg ctctcgatca
     5881 gcgccgtcac ccgagccgtc agccgcgaga ccccccgctc ctccggcggc agcgccgccg
     5941 ggtcgatcgg ctccgagtag gtgagccgat aacgccccgg cccgactcgc aggcagacca
     6001 cggggaggat cagcgcgccg gtccggtcgg ccagccgggc gatcgcggtc gtcgtccagg
     6061 cgaggcgccc gaagaacggg gtcaggagcg cgtcgggcgg gcgtgcgcgc tgatcgccca
     6121 ggatcccgac gacggcgccg gcccggagcg agcggaggat cgtccgcgcg gcgtccttct
     6181 tgttcacgac gtgtcccccc gtcctctcgc gcagcgcggt gagccggacg tcgaggagcg
     6241 ggttgtcgag cgggcgcgcc acgatcgtga ccggcatccc gttggccccg gtgacgaggt
     6301 tcgcccactc ccagttgccg aggtgcggcg tggcgaagaa gacgccgcgc ccggaggccg
     6361 cggccacgcg cgcgtgctcg agcccctcca cctcgacgcg cgcgaggagg gcctcgacgg
     6421 gcgccgcctc ggatcggacg aggtcggcgg cgatcgcgcc gaagtgggcg aagacctccc
     6481 gcgagatcgc ctcgcgctcc gcggccggct tctcggggaa ggcgagcgcg aggttgcggg
     6541 cgacgagccg gcggcgcctc cggtcgacga accggtagag gcggccgagc ccgcgtccga
     6601 ggccgtcggc gagggagagc gggagggtcc ggaagagcgt acgcaggagc aggtaggcgc
     6661 cgagctctac ccggtcccgg agcgcgccgt gcttgctggt cctcgtcacg agggcctccg
     6721 cgcgaggagc gagaggaggt cggagacgaa cgtcggctcg acgggccggg cctcgaccgc
     6781 gacgcggaag acggggaccc gtagccgttc gtccagcttc acggcgtcct tctccgtcgt
     6841 cacgacggcg gtcgcgccgc tctcctcgag cgtccgctcg atccgtccga cggaggccgg
     6901 gccgtacggc tcgtggtcgc ggaaggagag gagctcgacg ggctcgatgc cgatggaagc
     6961 gagcgtcgcg ccgaacgcct cggggcgggc gattcccgcc acggcgacgg ccgtcccggg
     7021 aggcaggtcc cccggctcga cggggaggcc gtcccggccg gtcacgccga ggacctccgt
     7081 ccgcgccctg tagaccggga cgcccggggc gaaccgccgg gcgatctcga gggccggacc
     7141 ggggtcggac tgctccgtct tcgtgacgac gaggaggtcg gccctcgcga gggacgagag
     7201 cggctcccgc aggcggccgg ccgggaggag cgcgcccccg gcgtcgggcg cggtcgcgtc
     7261 gaggaggacg acgtcgacgt cccgcgcgag ggcgacgtgc gagaagccgt cgtcgaggag
     7321 gtgcaggtcg accggcgtcc cgagcgtctc ggcccgcgcg gccgcgtcgc ggcgccgcgc
     7381 gtcggcgacg atcggcacgg acgggagcgc ccgggccagg agcgccggct cgtcgcccga
     7441 gtcggcgacg gtcgcgaagg tcccgcggcc gtccgagacg acgacggtgc cgcggcttcc
     7501 gcggccgtat ccgcgcgtca ggatcgacgg gtgccggccg cgccggagga gctccccggc
     7561 gagccaccgc acgaagggag tcttccccgt cccgccggcg gccacgttcc cgacggagac
     7621 caccggccgc gccgcgccgc gcacggcgag gaggcctcga cgatagagcg cgaggcggag
     7681 ggcgacgccg gcgccgtaga gggtggagag cggccggagg agcggcgtca cgcggccgct
     7741 ccgggacggc cgtcgaggaa gccgagcgcg agccgcacgg tccgctccgt cgcgccacgg
     7801 tgcgtcgcga agagcgccgc cgcggcctcc cgggcggccg ccctcgcggc cgggtcgtcg
     7861 aggaggcgcg cgagcgcggg ggcgacgccg gccgggtcgg gcaccccgac gaccgcggcg
     7921 ccggccgcct cgacgacgcg ccggatgttc cagacggagg ggccgaggac gaccgggagg
     7981 cccgcctgca gaggctcgag gacgttgtgc cccccccgcc gtccgaacgt gccgccgaga
     8041 agagcgacat cgccgagcgt gtaggccgag gcgagctccc cgagcgtgtc gaggaggaag
     8101 acgtcggccg ggccgtccgg cgccgggcgc gaccggcgga cgagccggag cccggcggcg
     8161 acggcgcggc gctccgcctc gtcccagctc tccgtccggc gcggggcgag gaggaggaac
     8221 ggcgtcccgg acgcgccccg ggcgagcagg cgcgggaggg agagcgcgag gtcgagctcc
     8281 tcctccgcga tcgagccggc gacgaggacc ggccgaccgg cggcgagctc ccggaacgag
     8341 gcggcgaacg ccggttcggc ggggaggggg cgatcgaact tcatgtcgcc cgcgacgtgg
     8401 accctctccc tcggcacgcc gacggcggcg aagcgctcgg cgtcctcctc gctgcgggcc
     8461 gcaacggcgg agagggcgtc gagaccgccg cgaaagagcg gccgggcggc gcgaagccgc
     8521 ctcacggagc gttccgagag acgcccgctc gccacgagga ccggcaggcc ccgccgccgg
     8581 gccgcccgaa ggaggacggg ccagagctcg gtctccatga ggacgaggag gcgtggcgtc
     8641 gccgagtcga ggagccggcg gaccgagacg gggaggtcga gggggaacgg ctgcagccgg
     8701 gcgggagggg cgaggtcgcg gaagcgcctc tcgaggaggc cgacgccggc cggggtcgag
     8761 gaggtgacga ggagcgacag ctccggacgc gcagagcgaa gggcgagcgc gagcgtgtgg
     8821 gccagctcga cctcgccgac cgagactccc tggacccaga cgtcgacccg cccggggacg
     8881 gcctcgggga ggagccttcg cgcgaggaga gccgtcaggc cggtcccccc ggccgacggg
     8941 cggtcggatc gggtcaaccg gcttccctgg aagaggttac gttctgcatc cgagaggccg
     9001 cattatatgc gccgcgccgc gccggagagc ggggcgggag cggacgaggc cgcctcgtag
     9061 acggggccgt gcgcggaacc gtagaaccga cggcatggcc attcagctgg tccacgtccg
     9121 ccgggtgcag gaggggacga cgatcgagcc gagggacgaa cggcacctcg cggcgctcgc
     9181 gaaggcgggc gacgaggacg ccttcgccga gctcgtccgc gggcgaaccg actccgtcgt
     9241 cgcgttcctc cgccggatgc tcggcgacac ggaggacgct cgcgacgtcg cgcagctgac
     9301 gttcctgcgc gtgtgggaga acctcccgcg ctacgacccg tcgtacgcct tctcgacgtg
     9361 gctcttccgg atcgccggga acctcgcgat cgacgccctc cgctcgaggg ggacgcgggc
     9421 ccggacgctc gccgagagct tccgcgtcgt gaaggggggg cttctcgccg cgagccccga
     9481 ggcgccgctg cgcctctcgc aggaggaggt ccgccgggtc ttcgaggcgt gtgcgtcggt
     9541 cctgtcggag aagcagcggc tcgtcttcgt cctccgcgag atggaggaga aggagagccg
     9601 cgaggtggcc gagatcgtcg gctgccggga gtcgaccgtg aggaaccacc tcttccaggc
     9661 ccggcgcctc ctgcgcgagg agctgaagcg gcgcttcccg gagtacgtcc ccgagtcgca
     9721 ccggagcgcc gagggagcat gagcgtgaaa gagcccgcct gcgaccgcct cgacgacctc
     9781 ctcgcgcggc gggaggagga tcgcctcacc ccggccgagg aaggcttcct ccgggggcac
     9841 ctcgcctcct gcgaggtctg cgccgccgcg gcgctgcgcc gcgacccggt cctcctcttc
     9901 gcccgggcgg ccgcgccgga gccgctctcc gccgacgcgc gggagcggtt cgtcagcggc
     9961 gtcctcgcgg ccgccgcgac cgcgaaggcc ggccgccgac tccggagagc ccacacccgg
    10021 cccgtcctcc ggctcgccgc ttccctcctc ctcgccgcct ccgtcgcggg cgtctggctc
    10081 gcgcgggagg cggggaacgg ccccgccgag gcgccccgcc ccgacctcgt cgccgccgcg
    10141 gacgagggcg tccgcacgcc gcccgacgtc gtccccgcgg tcgaggacct cggcggcgac
    10201 gcgtccgtcg tctaccagtt cccgtcgacc cggccgggcg agccgacggt cgtcttcgtc
    10261 gtcgaccgga acgccgacat ctgatgcgcg ccccgctctt cccgggcctc ctcgcggccg
    10321 cgctcctcct cgccgccccg gccgccgcga ggacgcccgc gccgagaccg acggcggggc
    10381 cggacgtcgt ccgggtcttc gtcctgaagc accggaaggc ggaggacgcg ttcctcctcg
    10441 tccgcccgct cctcggcgca cgcgggacga tcgtcctcca gacggcgacg aacgccctga
    10501 ccgtgaggga ctccgtccgc accgtcgaga gggtcgcgca ggcggtctcg gccttcgacc
    10561 ttcccccgag ggcgatcgcc gtctccgtct cgctcatccg ggcgacgcac gcgcccggcc
    10621 cgggcggccc gccggcgccc gtggccgagc ggttccgcgg ggtggccgac cgcctccgga
    10681 gcctcttcaa cttcaccgag tacgcctccc tcgacgaggt cgtcctgcag gggctcgagg
    10741 gcgaccccgt ctcctggacc ctcgggggga gcttccggat cgacttcctt ctcgagccgg
    10801 ggggcgggcg cgagagcgtt cgcctccgg
//
LOCUS       DAOTEZ010000102        10781 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4316_length_10781_cov_5.375070, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000102 DAOTEZ010000000
VERSION     DAOTEZ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10781
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4316_length_10781_cov_5.375070"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1960)
                     /locus_tag="P5164_07230"
     CDS             complement(<1..1960)
                     /locus_tag="P5164_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HRY43724.1"
                     /translation="MPATKRLSPATAASAAALLLVLCLPAAPVRAENALWLRAAAISP
                     DGKTVAFSYRGDLWTVPVAGGAATPITVAAAYDTAPVWSPDGKQIAFASDRYGNFDVF
                     VMPATGGEAKRLTYHSADDTPTSFTPDGKAVLFSSARLDAAKNVQFPTGAQPELYQVA
                     LTGGMPGQLLSTPALYAVWDKAAERLAYSDVKGYEMEWRKHDNSSFARDVWVFERKSG
                     KHTRLTAFGFDDRQPVWSPDQKSLYYLSEKSGTFNVWKLDLADPGKPVQVTTHETHPV
                     RFLSASSAGDLCYTWDGEIWVRPAGANESRKLSVTAASDRRDLEVAPVDVASDANELE
                     LSPNGKEVAFVARGEVFVASVEHGATRRITDTPGQERSVSFSPDGKSLLYAGERDGSW
                     NVYRTDRTDPEETAFFNATALKESAVVATPAEEFQPAFSPDGKEVAYLEERTTLKVVN
                     LATGKSRIVLPGDLNYSYADGDQWYQWSPDGKSFAVQFLSPTRWSGEVGIVDAAGGKP
                     VFNVTRSGYEEEAPKWARKGEVLFWASDRFGTRQHSGYPREQDVFAAFLTKKAWDRFH
                     LDEAAYAQLLEKEKEADKKKEKDADKGKDGKKVAGKDPNKDEDKKADGDVKLPEPVAF
                     ELDGLDKRIARLSLHSASIDDAALTPDGE"
     gene            complement(2030..2998)
                     /locus_tag="P5164_07235"
     CDS             complement(2030..2998)
                     /locus_tag="P5164_07235"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006992457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HRY43725.1"
                     /translation="MSVPRVHLAHLPTPVEELPRLSEALGGPRLLVKRDDLTGLALGG
                     NKTRKLEFLVAEAEARGARTLVTAGALQSNHCRQTAAAAARFGMGCVLVLTGETPGSP
                     TGNHLLDLLFGARVVAVADRADRDRVLAETVAALSAEGAAPYLVPYGGSSPTGALGYA
                     FAMEELLAQGVEADAIVFGTSSGGTHAGLLLGQRLFGFRGKVLGISIDEPEELLKAHV
                     SGLASDASERLGPRISFSPADVLADDRYCRAGYGVLTDLEREAIRLFARHEGLLLDPV
                     YTGRAAGGLIDLVRKGVFRRDERVLFWSTGGQPALFSERYQSALLS"
     gene            complement(2995..3333)
                     /locus_tag="P5164_07240"
     CDS             complement(2995..3333)
                     /locus_tag="P5164_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005995784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HRY43726.1"
                     /translation="MPRPCCRRRIAGAPPAAVFKPAGIPGRLLEEIVLGLDGLEAIRL
                     ADLEGLYQEEAAARMGVSRATFGRIVAEARRRVAEALVEGKLLRIEGGPVSESKEGAE
                     AAPVPPKAKR"
     gene            complement(3364..3867)
                     /locus_tag="P5164_07245"
     CDS             complement(3364..3867)
                     /locus_tag="P5164_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRY43727.1"
                     /translation="MGTRGREIVGIDVEKVIEMLNAAYASEWLAYYQYWLGAKLIKGP
                     MKDAVAAELNLHATEELNHAVLVANRIIQLGGTPLLDPHEWFDKSPCAYDAPKDPYVS
                     VLLDENIAGEQCAIDTYKKLMDVTKDKDMVTYNLALTILEQEVEHEEDLQSLKEDFDL
                     LFSRGNK"
     gene            complement(3978..4781)
                     /locus_tag="P5164_07250"
     CDS             complement(3978..4781)
                     /locus_tag="P5164_07250"
                     /inference="COORDINATES: protein motif:HMM:NF024823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY43728.1"
                     /translation="MRRVRLLPVVALAFAAIASALPAAAQEEHRATDAQRLRRYKGAI
                     AKYERALKLLEKNDRDRALDALAVSIEEMPDFPDSHFLRARLLYGGKEYSRALEEVVA
                     ARAAYERTADLRAEMQADRRKALTERLRAKDAAISEQSGRLAKASADQRQQIERLVAR
                     LQGERFDIERELHEHRPDPQGVPARYSSLHGNVLLRLGRDAEAIERYEEALKSDPAWG
                     EAANNLASLYHLAGKNERALEIVIQAEKLGAPLHPELKKSIEAALARRP"
     gene            4918..5946
                     /locus_tag="P5164_07255"
     CDS             4918..5946
                     /locus_tag="P5164_07255"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HRY43729.1"
                     /translation="MVDALLALVPVVLFLAVLVLMDSFKLVPLRGVVLLILAGMAAAF
                     VAMRLHGGLLDGGRIPASVLSRYVAPLTEEVLKALPVVWMLARKRIGFLVDAAILGFA
                     VGTGFALVENVEYLRALGESSVFLWIVRGFGTAVLHGGTTSILAVLARALEERRPRAG
                     LAVVLPGLALAIAVHSLFNHFLLPPIVATLALFVTLPVLFVAVFVRSEEATRAWLGPD
                     FDSELETLRTTLGRDVAENRVGKYLLSLRSRFEGAVVADMMCLLRLHLELSIRAKAML
                     MAREAGFSMEVDDTVRAKLTELRYLERSIGPTGLLALQPMLRVSSRHLWQIWMLETGS
                     GTGRRGRA"
     gene            5989..6525
                     /locus_tag="P5164_07260"
     CDS             5989..6525
                     /locus_tag="P5164_07260"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRY43730.1"
                     /translation="MSRDESDAELARRCVGGDPEAFRPLVERYQKVLYNVAYRMVHDR
                     EDARDLAQGALVKAYRKLGSFDPERPFFSWLYRILVNDALSFLEKRRPTAPLEAERDA
                     GAPGGPHEELERRERERRIQAALRELAPDSRQVIVLRYFADLSYAEMSEVLAIPEKTV
                     KSRLFTARQRLAGSLGGR"
     gene            6530..7297
                     /locus_tag="P5164_07265"
     CDS             6530..7297
                     /locus_tag="P5164_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43731.1"
                     /translation="MSEPGREELLHAELDGTASAADSARLRSLLEEEPELAARLEALR
                     RASGELERAERLEPPPELLEGVMAAVRERSRAEAVRPGWLEALRGLLAPVPLAACAAA
                     LLVGVVLGVLLPAGFGASPAERESLSGTALPHGRLGESADARSVAFRTWEVSGEATAR
                     RAGELLVVEVRLDARRPLEVRLVLDGAEVRPRSFERVGSGEADVVMGRGEVRIAHPGG
                     AGRYRIGLEVGPRSAGAVRLFLGDRESGALPFGRMGR"
     gene            7413..8390
                     /locus_tag="P5164_07270"
     CDS             7413..8390
                     /locus_tag="P5164_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43732.1"
                     /translation="MKKSVVLTCVAIVAIGAIAWFALKGLPPSEEGAEGTVGAARRHQ
                     SQQIGKSDVVIEDAGVQQFMQSDVYDKMVKDPKFVEAMKSDAFQAFLKSDAFAFLKSD
                     ASQSVLRSDAFQSAMKSDAFGIEIFKSDAFQQALKSDAFYVVLKNDAFKEALKNDAFQ
                     SALKSDAFQAALKSDAASLSEWINKSDAFKAALKSDAFAGFSKSDAFLSALKSDAFQS
                     ALKSDAFMQALKSDAFQTVLKSDAFQAALKNDAFQSALKNDAFQSALKNDAFLSALKN
                     DAFQSALKSDAFLGALKSDAFQAALKSDAFREALKNDAQLMKSDAQAVH"
     gene            8495..>10781
                     /locus_tag="P5164_07275"
     CDS             8495..>10781
                     /locus_tag="P5164_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43733.1"
                     /translation="MARSVLLAALSAALLLSSAPAAAQLEVLETRDLRLVSLGIGQAY
                     IVPHVARSFENSLRFQRKLFGYEPSGKVTVFLNDFADFGNAGVSVIPRNGMMFQIAPS
                     SFHYEALPSNEQVNWTMNHELVHVAAYDQETKKDRAFRRLFQGKVSTVAEQPETVLYT
                     WLTAPRAFSPRWYHEGIATFVETWMAGGRGRAQSAWDEMVFRAMAKDGSRFYDPLGLV
                     SEGTKVDFQLEANSYMYGGRFFSFLGYRYSPEHLVRWVARTEGSAAFYAERFREVFGT
                     SIPEAWSEWIAFEKEFQAKNLEAIRTYPITPYRDLSPRSQALGGVSRGYYDPDARKIY
                     AAFNYPGVVAHLGAISVDDGSIERIAEVKDPLGYFVTSLAFDPDSKTLFYTTDNHEYR
                     DVCSLDPRTGRSKVLLREARIGELVFNRADRSLWGVRVLNGITSLVRIPPPYDEWKLV
                     RSWPYGEVPYDLDLSPDGSLLSASVSQVDGQHTLRVMKTAALLEGDATPVAVTDFEAA
                     IPMNFVFAPDGKSLYGSSFYTGVANLFRFDLATGEKVALSNTETGFFRPLPMPDGSLV
                     AFRFTGEGFVPAKVEARVTEDVQPITFFGNEVFEKHPVLGEWKVPPPSSVDLEPLVTS
                     RGPYRAFRSVGLESLYPVLQGYKDSAAVGVRLNFSDPVQLHKASLTASYSPDGAVPGD
                     ERLHVDARYERYDWSARFRWNAGDFYDLFGPTKTSLKGWSLGTTWQKALIWDQPRELR
                     LKVGATYYGGLDRLPDFQEVAT"
BASE COUNT         1462 a         3634 c         3944 g         1741 t
ORIGIN      
        1 tctcgccgtc gggcgtgagc gccgcgtcgt cgatgctcgc ggagtggaga gagaggcggg
       61 cgatccgctt gtcgaggccg tccagctcga aggcgaccgg ctcggggagc ttcacgtcgc
      121 cgtcggcctt cttgtcctcg tccttgttcg ggtccttgcc cgcgaccttc ttcccgtcct
      181 tccccttgtc ggcgtccttc tccttcttct tgtccgcctc cttctccttc tccagaagct
      241 gcgcgtaggc ggcctcgtcg aggtggaagc ggtcccaggc cttcttcgtc aggaaggcgg
      301 cgaagacgtc ctgctcgcgc gggtagccgg agtgctggcg cgtgccgaaa cggtcggagg
      361 cccagaagag gacctcgccc tttcgggccc acttcggcgc ctcctcctcg tagccgctcc
      421 gggtgacgtt gaagaccggc ttgccgccgg cggcgtcgac gatgccgacc tcgcccgacc
      481 agcgggtcgg gctgaggaac tggacggcga agctcttccc gtcgggcgac cactggtacc
      541 actggtcgcc gtcggcgtag gagtagttca ggtcgccggg gaggacgatg cggctcttgc
      601 cggtggcgag gttgacgacc ttcagcgtcg tccgctcctc gaggtacgcg acctccttgc
      661 cgtcgggcga gaaggcgggc tggaactcct cggcgggggt ggcgacgacg gccgattcct
      721 tcagagcggt ggcgttgaag aaggcggtct cctcggggtc ggtccggtcg gtccggtaga
      781 cgttccagct gccgtcccgc tcgccggcgt agaggaggct cttgccgtcg ggggagaagc
      841 tgacggagcg ctcctggccc ggcgtgtcgg tgatgcggcg ggtcgcgccg tgctcgacgg
      901 aggcgacgaa gacctcgccc cgggcgacga aggcgacctc cttgccgttc ggggagagct
      961 cgagctcgtt cgcatcggag gcgacgtcga cgggggcgac ctcgaggtcg cgccggtcgg
     1021 aggcggcggt gacggagagc ttccggctct cgttcgcgcc ggcggggcgg acccagatct
     1081 cgccgtccca cgtgtagcag agatcgcccg cggaggaggc ggagaggaag cggacggggt
     1141 gggtctcgtg cgtcgtgacc tgcacgggct ttccgggatc ggccaggtcg agcttccaga
     1201 cgttgaacgt gccgctcttc tcggagaggt agtagagcga cttctggtcg ggcgaccaga
     1261 cgggctggcg gtcgtcgaag ccgaacgcgg tcagccgcgt gtgcttcccg ctcttcctct
     1321 cgaagaccca gacgtcgcgc gcgaacgacg agttgtcgtg cttgcgccac tccatctcgt
     1381 aacccttcac gtccgagtag gcgagccgct cggcggcctt gtcccagacg gcgtagaggg
     1441 ccggggtgga gaggagctgc cccggcatgc cgcccgtcag ggccacctgg tagagctcgg
     1501 gctgcgcgcc ggtggggaac tggacgttct tcgcggcgtc gaggcgggcc gagctgaaga
     1561 ggacggcctt gccgtcggga gtgaagctcg tcggcgtgtc gtcggcggag tggtacgtga
     1621 ggcgcttcgc ctcacccccg gtggccggca tgacgaagac gtcgaagttg ccgtaccggt
     1681 cggaggcgaa ggcgatctgc tttccgtcgg gcgaccagac cggcgcggtg tcgtaggccg
     1741 cggcgacggt gatcggcgtc gcggcgccgc cggcgaccgg gacggtccag aggtcgcccc
     1801 ggtagctgaa ggcgaccgtc ttcccgtcgg gggagatcgc cgcggcacgg agccagaggg
     1861 cgttctcggc ccggacgggg gcggcgggaa ggcagaggac gagaaggagc gccgcggcgg
     1921 aggccgcggt ggcgggggag agacgttttg tcgctggcat gctgctcact ccagagtcgt
     1981 ttacgcccga gcgggcctcg ggtttcgttc cggagcgcgt caggagccgt caggacagga
     2041 gggcggactg atatctctcg gagaagaggg ccggctggcc gccggtgctc cagaagagga
     2101 cccgctcgtc gcgccggaag acgcccttcc gaacgagatc gatcagccct ccggcggccc
     2161 gcccggtgta gacggggtcg aggaggagcc cttcgtgccg ggcgaagagg cggatcgcct
     2221 cgcgctcgag gtcggtcagg actccgtagc cggcgcggca gtagcggtcg tcggcgagga
     2281 cgtcggcggg ggagaaggag atccgtgggc cgagccgctc gctcgcgtcg gacgcgaggc
     2341 cggagacgtg cgctttcagc agctcctcgg gctcgtcgat gctgatcccg agcaccttcc
     2401 cccggaaccc gaagagacgc tggccgagga gaagcccggc gtgggtcccg ccgctcgagg
     2461 tgccgaagac gatcgcgtcg gcctcgactc cctgtgcgag gagctcctcc atggcgaagg
     2521 cgtagccgag ggcgcccgtc gggctgcttc cgccgtacgg gacgaggtag ggggccgccc
     2581 cctcggccga gagcgccgcg accgtctccg cgaggacgcg gtcccggtcg gcgcggtccg
     2641 ccacggcgac gacgcgggct ccgaagagga ggtcgaggag gtggtttccg gtgggcgagc
     2701 cgggcgtctc gcccgtcagg acgaggacgc accccatgcc gaagcgggcg gctgcggcgg
     2761 ccgtctgccg gcagtggttc gactggagcg cccccgcggt gacgagtgtc cgcgccccgc
     2821 gcgcctcggc ctcggcgacg aggaactcca gcttgcgggt cttgttcccg ccgagggcga
     2881 ggccggtcag gtcgtcgcgc ttgacgagga ggcgcgggcc gccgagcgcc tcggagaggc
     2941 gggggagctc ctcgaccggc gtcgggaggt gggcgaggtg gacccgcggg acgctcatcg
     3001 cttcgccttc gggggaacgg gggcggcctc ggcgccttcc ttggactccg agaccggccc
     3061 cccttcgatc cggaggagct tcccttcgac gagcgcttcc gcgacgcggc ggcgcgcctc
     3121 cgcgacgatc cgcccgaacg tcgcccgcga gacccccatc cgcgccgccg cctcctcctg
     3181 gtagagcccc tcgaggtcgg cgaggcggat cgcctcgagg ccgtccagcc cgaggacgat
     3241 ctcctcgagg aggcgccccg ggatccccgc cggcttgaag acggcggccg gcggggcgcc
     3301 tgcgatccgg cggcggcagc aggggcgggg catctctcct cgcggggcgg cggcgggacc
     3361 ggcctacttg ttgccgcggc tgaagaggag gtcgaagtcc tccttcaggc tctggaggtc
     3421 ctcctcgtgc tcgacttcct gctcgaggat cgtgagcgcc aggttgtacg tcaccatgtc
     3481 cttgtccttt gtgacgtcca tcagcttctt gtaggtgtcg atggcgcact gctcgccggc
     3541 gatgttctcg tccaggagga cggagacgta cgggtccttc ggggcgtcgt aggcgcaggg
     3601 gctcttgtcg aaccactcgt gcgggtcgag gagcggcgtg ccgccgagct ggatgatccg
     3661 gttcgcgacg aggacggcgt ggttcagctc ctcggtggcg tgcaggttca gctccgcggc
     3721 gacggcgtcc ttcatcggtc ccttgatcag cttggcgccg agccagtact ggtagtaggc
     3781 gagccactcg ctcgcgtagg cggcgttcag catctcgatg accttctcga cgtcgatgcc
     3841 gacgatctcg cgtcccctgg tgcccatcgg tacctcctcg cttcgggcgg ggtcgggcgc
     3901 gaacgccgcc ccggccggcg actcgtaagc atatgcccga ttgggggccg ggggaaccgg
     3961 aggagcggcc ccgggagcta cggccggcgg gccagggccg cctcgatcga cttcttcagc
     4021 tcgggatgga gcggagcgcc cagcttctcg gcctggatga cgatctcgag cgctctctcg
     4081 ttcttccctg cgaggtggta gaggctcgcg aggttgttcg ccgcctcgcc ccacgccggg
     4141 tcggacttca gcgcctcctc gtagcgttcg atcgcctcgg cgtcccgtcc gaggcggagg
     4201 aggacgttcc cgtggaggct gctgtagcgg gccggcacgc cctgcgggtc gggccggtgc
     4261 tcgtggagct cgcgctcgat gtcgaaccgt tcgccctgga ggcgggcgac gagcctctcg
     4321 atctgctgtc tctggtcggc ggaggccttc gcgaggcgtc cggactgctc cgagatcgcg
     4381 gcgtccttcg cgcgcagcct ctccgtcagc gccttccggc ggtcggcctg catctccgcg
     4441 cgcaggtcgg cggtcctctc gtaggcggcc cgtgcggcga cgacctcctc gagcgcgcgg
     4501 gagtactcct tcccgccgta gaggagccgc gcgcggagga agtgcgaatc cgggaagtcg
     4561 ggcatctcct cgatgctgac ggcgagggcg tcgagcgccc ggtcgcggtc gttcttctcg
     4621 aggagcttca gcgccctctc atacttcgcg atcgcgccct tgtagcgccg gagccgctgg
     4681 gcgtccgtcg cgcggtgctc ctcctgcgcc gcggcgggga gggcggaggc gatcgccgcg
     4741 aaggcgaggg cgacgacggg gagcaggcgg actcgtctca tgccgatcct tcgcctcctc
     4801 gcttcgggcg gggtcgaacg cggacgccgc cccggtcgac tgctcgcaag catatgcccg
     4861 attgagggcc tggggaaccg gccgggccgg cgatcggcgg ctatcatccg ctcgctggtg
     4921 gtcgacgctc ttctcgcgct cgtgccggtc gtcctctttc tggccgtgct ggtgctgatg
     4981 gacagcttca agctcgtccc gctgcgcggc gtcgttctcc tgatcctcgc cgggatggcc
     5041 gcggccttcg tcgcgatgag gctgcatggc gggctcctcg acggcggccg gatcccggct
     5101 tcggtcctgt cccgctacgt cgcgccgctg acggaggagg tcctcaaggc tctccccgtc
     5161 gtctggatgc tcgcccggaa gcggatcggc ttcctcgtcg acgcggcgat cctcggcttc
     5221 gccgtcggga cgggcttcgc cctcgtcgag aacgtcgagt acctgcgggc gctcggggag
     5281 agcagcgtct tcctctggat cgtgaggggc ttcgggaccg ccgtcctcca cggcgggacg
     5341 acgtcgatcc tcgccgtcct cgcccgagcc ctcgaggagc ggcggccccg ggccggcctc
     5401 gcggtcgtcc tgcccggcct ggccctggcc atcgccgtcc actctctctt caaccacttc
     5461 ctcctgccgc cgatcgtcgc gacgctcgcc ctcttcgtca cgctcccggt cctcttcgtc
     5521 gccgtcttcg tgaggagcga ggaggcgacg cgcgcgtggc tcggacccga cttcgactcg
     5581 gagctcgaga cgctccggac gacgctcggc cgggacgtgg ccgagaaccg cgtggggaag
     5641 tacctcctct ccctgaggtc gcgcttcgag ggggccgtcg tcgccgacat gatgtgtctc
     5701 ctccggctcc acctcgagct gtcgatccgg gcgaaggcga tgctgatggc gcgggaggcc
     5761 ggattctcga tggaggtcga cgacacggtc cgggcgaagc tgaccgagct ccggtacctc
     5821 gagcggtcga tcgggccgac ggggctcctc gccctccagc cgatgctccg ggtctcctcg
     5881 cggcacctct ggcagatctg gatgctcgag acgggaagcg gcacgggccg tcgggggagg
     5941 gcgtgacgag ccggcggggt gccaactttt ccgggggggc ggcggtactt gagccgggat
     6001 gagagcgacg ccgagctcgc gcgacgatgc gtcgggggcg acccggaggc cttccggccg
     6061 ctcgtcgagc ggtaccagaa ggtgctctac aacgtcgctt accggatggt ccacgaccgc
     6121 gaggacgctc gggacctcgc gcagggagcc ctcgtgaagg cctaccgaaa gctcgggagc
     6181 ttcgacccgg agcggccgtt cttcagctgg ctctaccgga tcctcgtgaa cgacgcgctg
     6241 agcttcctgg agaagaggag accgacggcg cccctggagg cggagcggga cgccggcgct
     6301 ccgggcggac cgcacgagga gctggagagg cgcgagcggg agcgccggat tcaggcggct
     6361 ctccgggagc tcgcgcccga ttcccggcag gtgatcgtcc tccggtactt cgcggacctt
     6421 tcctacgccg agatgagcga ggtgctggcg atcccggaga agaccgtgaa gtcccgcctc
     6481 ttcaccgccc gccagcgcct ggccgggagc ctcgggggga ggtgagccgt tgagcgagcc
     6541 tggccgcgag gagctgctcc atgcggagct ggacgggacc gcctcggccg ccgactcggc
     6601 ccgcctgcgc tcgctcctcg aggaggagcc cgagctggcg gcccggctcg aggccctccg
     6661 ccgcgcttcc ggggagctgg agcgggccga gcggctcgag cccccgcccg agctcctcga
     6721 aggagtgatg gccgccgtcc gggagcgctc gcgcgcggag gccgtccggc cgggctggct
     6781 cgaggctctc cgcgggctcc tcgccccggt ccccctcgcg gcgtgcgcgg ccgcgctcct
     6841 tgtcggcgtc gtcctgggcg ttctcctccc ggccggcttc ggcgcatcgc ccgccgagag
     6901 ggagtctctt tcggggacag ctctgccgca cgggcgcctc ggcgagtccg cggacgcgcg
     6961 ctcggtcgcg ttccggacgt gggaggtttc gggagaggcc acggcgcggc gggcggggga
     7021 gctcctcgtc gtggaggtgc gcctcgacgc gcggcggccg ctcgaggtcc ggctcgtcct
     7081 ggacggtgcg gaggtccggc cgcgctcttt cgaaagggtc ggttcgggag aggcggacgt
     7141 cgtgatggga cgcggcgagg tccgcatcgc gcacccgggc ggcgcggggc gctaccggat
     7201 cggcctcgag gtcgggcccc gctcggcggg ggccgtccgc ctgttcctcg gcgaccgcga
     7261 gagcggcgcg ctcccgttcg ggagaatggg gcgatgatcc gcagggagcc ggcgggactc
     7321 ctccaacctt tttcgccgag ggcggtactt ccattcgatt cggaacgaca cggaggcgag
     7381 cccgagagga gcggctccgg aggagaggga cgatgaagaa gagcgtggtc ctgacgtgcg
     7441 tcgcgatcgt ggccatcgga gcgatcgcat ggttcgccct gaaggggctt cccccgtccg
     7501 aggagggggc cgaggggacg gtcggggcgg cccggcgcca ccagtcgcag cagatcggga
     7561 agtccgacgt cgtcatcgag gacgcgggag tccagcagtt catgcagtcc gacgtctacg
     7621 acaagatggt caaggacccg aagttcgtgg aggcgatgaa gagcgacgcc ttccaggcct
     7681 tcctgaagag cgacgccttc gcgttcctga agagcgacgc ttcccagtcc gttctcagga
     7741 gcgacgcgtt ccagtcggcg atgaagagcg acgccttcgg gatcgagatc ttcaagagcg
     7801 acgcgtttca gcaggcgctg aagagcgacg cgttctacgt cgtcctgaag aacgacgcct
     7861 tcaaggaagc cctgaagaac gacgcgttcc agtcggctct gaagagcgac gcgttccagg
     7921 cggcgctgaa gagcgacgcg gcgtcgcttt ccgagtggat caacaagagc gacgccttca
     7981 aggcggcgct gaagagcgac gctttcgccg gcttctccaa gagcgacgcg ttcctctcgg
     8041 ctctgaagag cgacgcgttc cagtcggccc tgaagagcga cgccttcatg caggcgctga
     8101 agagcgacgc gttccagacg gtgctgaaga gcgacgcctt ccaggcggcg ctgaagaacg
     8161 acgcgttcca gtcggccctg aagaacgacg cgttccagtc ggcgctgaag aacgacgcct
     8221 tcctctcggc gctgaagaac gacgcgttcc agtcggcgct gaagagcgac gccttcctgg
     8281 gggcgctgaa gagcgatgcg ttccaggcgg cgctgaagag cgacgcgttc cgggaggcgc
     8341 tgaagaacga cgcccagctg atgaagagcg acgcccaggc ggtgcactga tgggcggctc
     8401 gtccggattc gccccgcggt aggccccggc tcccggccgg ccggaggcgc gccaggcgcg
     8461 cccccggccg ttttcgttct cgcggaggtg ctcgatggct cgatcggtcc ttctcgccgc
     8521 cctttcggcg gcgctcctgc tctcttcggc tcccgcggcg gcccagctgg aggtcctcga
     8581 gacgcgggac ctgcgcctcg tctccctcgg gatcgggcag gcgtacatcg tcccgcacgt
     8641 ggcgcgctcg ttcgagaact cgctgcgctt ccagcggaag ctcttcggct acgagccgtc
     8701 cgggaaggtc acggtcttcc tgaacgactt cgccgacttc gggaacgccg gcgtgagcgt
     8761 catcccgcgg aacgggatga tgttccagat cgcgccgtcg agcttccact acgaggcgct
     8821 gccttcgaac gagcaggtca actggacgat gaaccacgag ctcgtccacg tggcggcgta
     8881 cgaccaggag acgaagaagg accgggcgtt ccgccggctc ttccagggca aggtgtcgac
     8941 ggtcgccgag cagcccgaga cggtcctcta cacctggctg acggcgccgc gggccttctc
     9001 cccgaggtgg taccacgagg ggatcgcgac gttcgtcgag acgtggatgg ccggcgggcg
     9061 cggacgcgcc cagagcgcct gggacgagat ggtcttccgc gcgatggcga aggacggcag
     9121 ccgcttctac gacccgctcg gcctcgtctc cgaggggacg aaggtcgact tccagctcga
     9181 ggcgaactcg tacatgtacg gcgggaggtt cttctcgttc ctcgggtacc gctactcccc
     9241 ggagcacctc gttcgctggg tggcccggac ggagggctcg gcggccttct acgcggagcg
     9301 gttccgcgag gtcttcggga cgtcgatccc ggaggcgtgg agcgagtgga tcgccttcga
     9361 gaaggagttc caggcgaaga acctggaggc gatccgcacc tacccgatca cgccgtaccg
     9421 cgacctgtcg ccgcgctccc aggcgctggg cggcgtgtcg cgggggtact acgacccgga
     9481 cgcgaggaag atctacgccg ccttcaacta ccccggcgtg gtggcccacc tcggggcgat
     9541 ctcggtggac gacggctcga tcgagaggat cgccgaggtc aaggacccgc tggggtactt
     9601 cgtcacgtcg ctcgccttcg acccggattc gaagacgctc ttctacacga cggacaacca
     9661 cgagtaccgc gacgtctgct cgctcgaccc gcgcaccggc cgctcgaagg tcctgcttcg
     9721 ggaggcgcgg atcggcgagc tcgtcttcaa ccgggccgac cgctcgctct ggggagtccg
     9781 cgtcctgaac ggcatcacgt cgctcgtgag gatcccgccg ccctacgacg agtggaagct
     9841 cgtccgatcc tggccgtacg gggaggtccc gtacgacctc gacctctcgc ccgacggcag
     9901 cctcctctcc gcctcggtgt cgcaggtcga cggccagcac accctgcgtg tgatgaagac
     9961 cgcggccctc ctcgagggag acgcgacccc ggtcgccgtc accgacttcg aagcggccat
    10021 cccgatgaac ttcgtattcg cgcccgacgg gaagagcctc tacgggagct cgttctacac
    10081 gggcgtcgcg aacctcttcc gcttcgacct cgcgacaggc gagaaggtgg ccctcagcaa
    10141 caccgagacc ggcttcttcc gcccgctgcc gatgcccgac gggtcgctcg tggccttccg
    10201 cttcacgggg gagggcttcg tcccggcgaa ggtggaggcg cgcgtcacgg aggacgtcca
    10261 gccgatcacc ttcttcggaa acgaggtctt cgagaagcac ccggtgctcg gggagtggaa
    10321 ggtcccgccc ccctcctcgg tcgacctcga gcctctcgtc acctcccgcg ggccgtaccg
    10381 ggccttccgg agcgtcgggc tcgagtctct ctacccggtc ttgcagggct acaaggactc
    10441 ggcggcggtc ggcgtccggc tgaacttctc cgacccggtc cagctccaca aggcgtccct
    10501 caccgcctcc tattcgccgg acggcgcggt cccgggggac gagcggctcc acgtcgacgc
    10561 ccggtacgag cgatacgact ggagcgcacg cttccgctgg aacgcggggg acttctacga
    10621 cctcttcggc ccgacgaaga cgagcctgaa gggctggtcc ctcgggacga cctggcagaa
    10681 ggcgctgatc tgggaccagc cgcgcgagct ccggctcaag gtgggcgcga cctactacgg
    10741 cggtctcgac cgccttccgg actttcagga ggttgcgacc t
//
LOCUS       DAOTEZ010000103        10720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4346_length_10720_cov_5.684388, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000103 DAOTEZ010000000
VERSION     DAOTEZ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10720
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4346_length_10720_cov_5.684388"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..3126)
                     /locus_tag="P5164_07280"
     CDS             complement(232..3126)
                     /locus_tag="P5164_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRY43734.1"
                     /translation="MNGKRLWLLVLLVPFLLAVQADAQGLPTGTLNGRVAEAEGLALP
                     GVTVTAKSPALQGTRTAVTNVNGDFVIPNLPPGDYIVSFVMSGFQNVTRNVRVSAGQQ
                     TPLNTKMSIAAVAAEAMVVAQSETVSQTAQAATTYSTELTNKLPVARQILSSVVLSAG
                     VNSNGPGGNTTISGAQSFDNVITVNGVNIQDNIRGTPTNTLVIEDAIQETTTMTSGVS
                     AEYGRFTGGVINAVTKQGGNAFSGSFRVTLNNNDWQSQGPIAQTYVDKVVPTYEATFG
                     GPIIKDRVWFFGAGRYQKNEYSGALTAPLTGSFPRSDKDTRYEGKLTFTPFQNHTITG
                     SYTKGEVEQANYYFTSFPILETNGVIYDRSLPTDLVSINYNGVLSSNFFLEAQYAAKT
                     FTFKNSGSRFNELIKGTAVVSQDPFGQMFGAIFCAVCPGAGEKRDNENFLVKGTYFLS
                     TKSLGSHNVVVGYDNFASTRMSNNWQSGSSWLLFPTSVQTDGTNLYPIIDGNSYFIYA
                     PIPLASQGSDMLTHSLFLNDTWRLNDRFSFNIGLRWDKNDATDAAGKKTADDSAFSPR
                     LAVNWDVFGDGKLRATASYATYVGQIQEGIAGSGATGAGSPASYYYYWDGAPINDGAG
                     PWLPTDEVLRRMYAAWGVTGLNQFPNRAADLAIVPGVNLLIGDGLSSPKVNEYVLGFG
                     GTLGKNIVYRADVVRREYKDFYSTKKDMTTGQVTSELGDEFDLGYIINSNVPEREYTG
                     LHSSIAWRKGNLNLAANWTWSHMIGNFVGETSGSGPVSANFESYPEYINLKWNAPRGD
                     LSQDQRHRVRLVGTYDFRVGMIGITPGIVQSWDTGTPYGASGNVHVRSYVNNPGYVAP
                     PLNSAYYFTARDAYRTDDIWRTDLSVNLSANIGKFEVFVQPQMWNVFNADGLVTPNTG
                     ITTNIVRFNPFTEQPVEGVNWRKGANFGKGTSANAFQIPRRYFVSMGIRF"
     gene            complement(3635..6613)
                     /locus_tag="P5164_07285"
     CDS             complement(3635..6613)
                     /locus_tag="P5164_07285"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRY43735.1"
                     /translation="MKRLGLVFVLLMVAVIAIPAGAQEQTGVLTGRITADGGEALPGV
                     SVEIKGPSGTLVSVTDARGDYRFPRVSPGVYTITARLQGFQTYEASRVPVALGDRAIL
                     NVTLKISSVMETVVVTGERVQIAVGENSTAASMAAEQIALLPKGRDFTSVVAQAPGAS
                     NEAFAGGISIDGASGSENRFVIDGIDTTRPNDGLSGQGFITDFVEEIQVKSAGYSAEF
                     GGSLGGVINAITKSGTNEFHGWLGGYFSSSSLNGDRRPIAYESWPGFYRTFDKDDESR
                     FEPGFALGGPILKDSLWFYLGYSPTLRSIERTPYEKTTTAKQDITDHFFAGNIKGNIG
                     SKLVFKVSANVTPSETDGNLPALDGSTPDDADLDVVSKFPTSSYSAYADFVPTSNFYV
                     SGRVGLFETDTEITGIDAQNRMLFRNGVIPLPTTDPRYRPTGFATVPNDSFQSADEDN
                     WKRTSAGIDANLFVTALGSHSFKAGVQYEKIENSVSLGENGNYFEVRWGLPDRFGMGV
                     QGTYGSVGVRRFRTEGAAEASNVGLFIQDTWNVHPNLTLNIGVRTEQERIPNYGAQVD
                     PTLPKNAIEFDFKDKLAPRLGFSWDVTGKQQLKVYGSYGTYYDITKLEMPRGSFGADR
                     WIQHMYELNTLNWDTLFAACTRVDNTLGQNPCPTLVGHKARDLRAPGDPHDSIDPNLK
                     PMENQEIQLGVDYQLTKNSVVSARYVNKTLKNTIEDIGYLVFLPDGSSEEQYITGNPG
                     KGIVSGDPAGPIPPQAEAIRDYEAIELTFSRRFADSFSVRAAYVYSKLSGNYSGLASS
                     DEFGRTDPNVARYFDGLVYGYDSKGKLVDGVLNTDRPHAFEVQGLYQASWGTNIGLNF
                     SWASGSPVSTDGSYNGVNFFPNGRNDMGRLSSLTQTDLLLTHPFKIGGFDLEVSLNVL
                     NLFDQDTVTRIDNVKYFDDVCDVSEECDGSNDWYFSDLVPYDYSTYMDANHASKNPLY
                     GKPLTYQAPRTVRFGLKFVF"
     gene            7056..7850
                     /locus_tag="P5164_07290"
     CDS             7056..7850
                     /locus_tag="P5164_07290"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015188125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRY43736.1"
                     /translation="MRALSVCLSLAALAALAGVTLPAAAAVKTATVEYLDGDQTCEGF
                     LAWDDAVKGKRPGVVVVHEWMGLGDYAKERARQLAALGYVALAADIYGKGIRAKDAAE
                     AGRLAGRFKGDLPLLRSRARAALDTLAKDPRTDASRLFAIGYCFGGTTALELARSGAP
                     VAGVVSFHGGLATKDPADAKNVQGRVLVLHGAADPYVPPAEVAAFQKEMDDAKVDWQM
                     VFYAGAVHSFTNPAAGNDSSKGAAYDARADRRSWEAMKAFLAEGAK"
     gene            7907..10114
                     /locus_tag="P5164_07295"
     CDS             7907..10114
                     /locus_tag="P5164_07295"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRY43737.1"
                     /translation="MTPRLRTAALLLPLLALLALPGTASADEGQWPPEALATLGEARW
                     AELAARGLELGATDLWDGKGGGLLTAVLGLGGCTGAFVSEEGLFLTNHHCAFGAIQLA
                     STPEKNYLRDGFVARSRAEEVPARGGVGRVRILTRLSDVTDRVRGPKSAFARAKDDAA
                     REAAVEKARSEIVAECEKQPDRRCAVAAFEDGRTYRLMEQIEFRDVRLVYAPPRGVGD
                     FGGEVDNFRWPRHAGDFSLLRVWAAPDGSPAAHAPENVPYRPKRWLPVSSKGVSEGDV
                     VMIAGYPGRTQRYLTPSAVENLESWFYPLRSRTFTDLIEILETEGKKDPDTALRVSGA
                     VKGYGNGETNARGQVEGLARNGVAARAREEAKELEEFLAARKDLPAEWTAAPAELEKV
                     LARDREGQERRFFLEEIERLSSYLSSALSAVRRADERKKPDLEREPGFQERDLERARQ
                     QEKDRTLSLAPAATRKALAYLVEKAREASKDAPVRAFDEAFGKDAGRAAIEKRLAEMD
                     AATSLSDENVRLANVDATLASLSASADPYVKLAVGLHADLAAIRQKRKETNGALLVWR
                     PRYLSALEALRRSKGKAISPDANSTLRVSFATVKGYSPREAVTFTPRTSLAGVLEKAR
                     AEEPFAVPGKVLDAARAGRFGRWADPVLGSVPVGFLTDGDTTGGNSGSPTMNGKGEVV
                     GLNFDRVWENVAGDFGWRPERSRNVNVDLRYALWMMEEVDGAAHLVKELLPAK"
     gene            10111..>10720
                     /locus_tag="P5164_07300"
     CDS             10111..>10720
                     /locus_tag="P5164_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY43738.1"
                     /translation="MTTAKGSVERRFRARGCRARVGVPLCVAILLAAACRRDAPPRTV
                     EVLVPFLPASLDPYSDPRLVSRSIFSAIYEPLVAENDVGVRPALAESWTNPAPDTWLF
                     RIAPDATFHDRSPVTSREVVAAAHASRTARGSVASLADLRAIEVADARTVRFRTFQAP
                     EEFLLSVSALFITRRDADRFVGSGAYRVVAHTPDRIVLTRHDR"
BASE COUNT         1592 a         3514 c         3877 g         1737 t
ORIGIN      
        1 atgagcgacc gacgaagtcg gtcctcgact cgtcttctac agggagtctt gcgggctctc
       61 ctgatgtcgg tcgtctccgc cccttacgag agtccccccg gattcaatag cggggtgaag
      121 gcccaactgt ggaaggcgag tcacagcact tcgcgccatg ccgggcgcac acgacgagcc
      181 cccccggccc gaaggccggg ggggccgggg atacccggaa gaggcgttcc gctagaagcg
      241 gatgcccatg gagacgaagt accggcgcgg gatctggaag gcgttggcgc tcgtgccctt
      301 gccgaagttg gcgcccttcc tccagttcac gccctcgacc ggctgctcgg tgaaggggtt
      361 gaagcggacg atgttcgtcg tgatcccggt gttcggggta accaggccgt ccgcgttgaa
      421 cacgttccac atctgcggct ggacgaagac ctcgaacttg ccgatgttcg cgctgaggtt
      481 gacggagagg tccgtcctcc agatgtcgtc ggtccggtag gcgtcgcgcg ccgtgaagta
      541 gtaggccgag ttgagcggcg gggcgacgta gcccgggttg ttgacgtagg agcggacgtg
      601 gacgttaccg gacgcgccgt acggcgtgcc ggtatcccag gactggacga tgccgggggt
      661 gatcccgatc atcccgacgc ggaagtcgta cgtgccgacg aggcggacgc ggtgccgctg
      721 gtcctgcgag aggtcgccgc gaggagcgtt ccacttcagg ttgatgtact cgggatagct
      781 ctcgaagttc gcggagaccg ggccggagcc gctcgtctcg ccgacgaagt tgccgatcat
      841 gtgcgaccag gtccagttgg cggcgaggtt gaggttcccc ttgcgccagg cgatggacga
      901 gtggaggccc gtgtactcgc gctcggggac gttcgagttg atgatgtagc cgaggtcgaa
      961 ctcgtcgccg agctcgctcg tcacctggcc ggtcgtcatg tccttcttgg tggagtagaa
     1021 gtccttgtac tcgcggcgga cgacgtcggc gcggtagacg atgttcttgc cgagcgtgcc
     1081 gccgaagccg aggacgtact cgttcacctt cggggaggag agcccgtccc cgatgaggag
     1141 gttgacgccg gggacgatgg cgaggtcggc cgcccggttc gggaactggt tcaggccggt
     1201 gacgccccac gccgcgtaca tgcggcggag gacctcgtcg gtcggcagcc aggggccggc
     1261 gccgtcgttg atcggggcgc cgtcccagta gtagtagtac gaggccgggc tgccggcgcc
     1321 cgtcgcgccc gagccggcga tcccttcctg gatctggccg acgtaggttg cgtagctcgc
     1381 cgtcgcgcgg agcttgccgt cgccgaagac gtcccagttc acggcgaggc gggggctgaa
     1441 cgcgctgtcg tcagccgtct tcttgccggc cgcgtccgtc gcgtcgttct tgtcccagcg
     1501 gaggccgatg ttgaacgaga agcggtcgtt caggcgccag gtgtcgttga ggaagagcga
     1561 atgcgtgagc atgtcgctcc cctggctcgc cagcgggatc ggggcgtaga tgaagtagga
     1621 gttgccgtcg atgatcgggt agaggttcgt cccgtccgtc tgcaccgagg tcgggaagag
     1681 gagccaggag ctccccgact gccagttgtt ggacatgcgg gtcgaggcga agttgtcgta
     1741 cccgacgacg acgttgtgcg agccgaggct cttcgtggag aggaagtagg tccccttcac
     1801 gaggaagttc tcgttgtcgc gcttctcgcc ggcgcccggg cagacggcgc agaagatcgc
     1861 gccgaacatc tgcccgaacg gatcctggga gacgaccgcg gtccccttga tcagctcgtt
     1921 gaaacggctg cccgagttct tgaacgtgaa cgtcttggcc gcgtactggg cctcgaggaa
     1981 gaagttcgag gagaggacgc cgttgtagtt gatcgagacg aggtcggtcg ggagcgagcg
     2041 gtcgtagatg acgccgttcg tctcgaggat cgggaacgag gtgaagtagt agttcgcctg
     2101 ctcgacctca cccttcgtgt aggagccggt gatcgtgtgg ttctggaagg gggtgaaggt
     2161 gagcttcccc tcgtaccgcg tgtccttgtc ggagcgcggg aaggagccgg tgagcggggc
     2221 cgtaagagcg cccgagtact cgttcttctg gtagcggccg gcgccgaaga accagaccct
     2281 gtccttgatg atcgggccgc cgaacgtcgc ctcgtaggtc gggacgacct tgtcgacgta
     2341 cgtctgggcg atcggaccct gggactgcca gtcgttgttg ttcagcgtga cgcggaagga
     2401 gccgctgaag gcgttgccgc cctgcttcgt gacggcgttg atgacgccgc ccgtgaagcg
     2461 cccgtactcc gccgagacgc ccgacgtcat cgtggtcgtc tcctggatgg cgtcctcgat
     2521 gacgagcgtg ttcgtcggcg tgccgcggat gttgtcctgg atgttcacgc cgttgacggt
     2581 gatgacgttg tcgaacgact gcgcgcccga gatcgtcgtg ttgccgccgg ggccgttcga
     2641 gttgacgccg gcgctcagga cgaccgagga gaggatctgc cgcgcgaccg ggagcttgtt
     2701 cgtcagctcg gtcgagtagg tcgtcgcggc ctgcgccgtc tgggagacgg tctcgctctg
     2761 ggcgacgacc atcgcctcgg cggcgacggc ggcgatcgac atcttcgtgt tcagcggggt
     2821 ctgctgtccg gcagagaccc gcacgttcct cgtcacgttc tggaagcccg acatgacgaa
     2881 gctgacgatg tagtcgcccg ggggaaggtt cgggatgacg aagtcgccgt tcacgttcgt
     2941 gacggcggtc cgcgtgccct ggagcgcggg ggacttcgcg gtgaccgtca cgccggggag
     3001 cgcgaggccc tcggcctcgg cgacgcgccc gttcagggtg cccgtcggga gcccctgggc
     3061 atccgcctgc actgccagca ggaacggtac gaggaggacg aggagccaga gtcgctttcc
     3121 attcatgtcc taaatccctc cggaaaacat tcgttggtct tgcacgtgag ttctgggcat
     3181 cggcacacga gatcggggac gcaccccccg gcggccgggc cggcgggccc gcacccctcg
     3241 cggtgcggtg tcatcgctga cttcgactct ctcctcggac ctcctttccc cgcccggggg
     3301 accattcggg ggaacgtccc tgccaaggac tttccaaccc tgagccaaga aaccggagcg
     3361 cggtacgggt tttcggagcc aatgggcgcg gcattatagc gaccccgggg gcgacgcagg
     3421 cgcgggacgc gcttctgagc tgctgatcgc gctcatcttc cgcctccggg gcggacggga
     3481 ccggatccgt cctcatactg ttccggccag cccgatgaaa gatccgtgcc gcccgggaca
     3541 ggcaggatcc cgagccgctc acgcccccca ggaaagagaa aacggcggga gcgctcacgc
     3601 gctcccgccg tccggagccg gttgaccggc cgggtcagaa gacgaacttc agaccgaaac
     3661 ggaccgtccg gggggcctgg taggtgaggg gcttgccgta gagcgggttc ttcgacgcgt
     3721 ggttcgcgtc catgtaggtg ctgtagtcgt aggggaccag atccgagaag taccagtcgt
     3781 tcgagccgtc gcactcctcg gacacgtcac agacgtcgtc gaagtacttg acgttgtcga
     3841 tccgggtgac cgtgtcctgg tcgaagaggt tgaggacgtt gagggagacc tcgaggtcga
     3901 agcccccgat cttgaacggg tgggtgagga ggaggtcggt ctgggtcagg ctggagagcc
     3961 ggcccatgtc gttccggccg ttcgggaaga agttgacgcc gttgtacgag ccgtcggtcg
     4021 agaccgggct ccccgaggcc caggagaagt tgaggccgat gttcgtcccc caggaggcct
     4081 ggtagaggcc ctggacctcg aaggcgtgcg gccggtcggt gttgaggacg ccgtcgacga
     4141 gcttgccctt cgagtcgtag ccgtagacga gaccgtcgaa gtagcgggcg acgttcgggt
     4201 cggtccggcc gaactcgtcg gagctcgcga ggccggagta gttgccggaa agcttgctgt
     4261 agacgtaggc ggcgcggacc gagaagctgt cggcgaagcg gcggctgaac gtcagctcga
     4321 tcgcctcgta gtcgcggatc gcctcggcct gcggcgggat cgggccggcc gggtcgccgg
     4381 agacgatgcc cttgcccggg ttgccggtga tgtactgctc ctcggaggag ccgtcgggca
     4441 ggaagacgag gtagccgatg tcctcgatcg tgttcttgag cgtcttgttg acgtaccggg
     4501 cgctgacgac cgagttcttc gtgagctgat agtcgacgcc gagctggatc tcctggttct
     4561 ccatcggctt gaggttgggg tcgatgctgt cgtgcgggtc gccgggggcg cggaggtcgc
     4621 gcgccttgtg gccgacgagc gtcgggcagg ggttctgtcc gagcgtgttg tcgacgcgcg
     4681 tgcaggcggc gaagagcgtg tcccagttga gcgtgttcag ctcgtacatg tgctggatcc
     4741 agcggtcggc gccgaaggag ccgcgcggca tctcgagctt cgtgatgtcg tagtaggtgc
     4801 cgtaggagcc gtagaccttc agctgctgct tgcccgtgac gtcccaggag aagccgaggc
     4861 gcggggcgag cttgtccttg aagtcgaact cgatcgcgtt cttcggaaga gtcgggtcga
     4921 cctgggcgcc gtagttcggg atccgctcct gctcggtgcg gacgccgatg ttcagcgtca
     4981 ggttcgggtg gacgttccag gtgtcctgga tgaagagacc gacgttcgac gcctcggcgg
     5041 cgccctcggt ccggaagcgg cggacgccga cggagccgta ggtgccctgg acgcccatgc
     5101 cgaaccggtc cggaaggccc cagcggacct cgaagtagtt gccgttctcg ccgagggaga
     5161 ccgagttctc gatcttctcg tactggacgc ccgccttgaa ggagtgcgag ccgagggccg
     5221 tcacgaagag gttcgcgtcg atgccggccg acgttctctt ccagttgtcc tcgtcggcgc
     5281 tctggaacga gtcgttcggg acggtcgcga agccggtggg gcggtagcgc gggtcggtgg
     5341 tcgggagcgg gatgacgccg ttccggaaga gcatccggtt ctgggcgtcg atgccggtga
     5401 tctcggtgtc ggtctcgaac aggccgacgc ggccgctcac gtagaagttg ctggtgggga
     5461 cgaagtcggc gtaggccgag tagctcgacg tcgggaactt cgagacgacg tcgaggtcgg
     5521 cgtcgtccgg ggtcgagccg tcgagggccg ggaggttccc gtccgtctcg gagggggtga
     5581 cgttcgccga gaccttgaag acgagcttgg agcctatgtt ccccttgatg ttcccggcga
     5641 agaagtggtc ggtgatgtcc tgcttcgccg tcgtggtctt ctcgtacggc gtccgctcga
     5701 tcgaacggag cgtcgggctg tagccgaggt agaaccagag cgagtccttg aggatcgggc
     5761 cgccgagggc gaagcccggc tcgaagcgcg actcgtcgtc cttgtcgaac gtccggtaga
     5821 agcccggcca cgactcgtac gcgatggggc gccggtcgcc gttcagcgag gagctcgaga
     5881 agtagccgcc gagccacccg tggaactcgt tcgtgccgct cttcgtgatc gcgttgatga
     5941 cgccgccgag ggagccgccg aactcggccg aatagccggc cgacttgacc tggatctcct
     6001 cgacgaagtc ggtgatgaag ccctggccgg agaggccgtc gttcggacgg gtcgtgtcga
     6061 tcccgtcgat gacgaagcgg ttctccgagc cggaggcgcc gtcgatcgag atgccgcccg
     6121 cgaacgcctc gttggaggcg ccgggggcct gcgcgacgac cgaggtgaag tcgcgcccct
     6181 tcgggaggag cgcgatctgc tcggcggcca tcgaagccgc cgtggagttc tcgcccacgg
     6241 cgatctggac ccgctcgccg gtgacgacga ccgtctccat gaccgagctg atcttcagtg
     6301 tgacgttcag gatcgcgcgg tcgccgaggg ccaccggcac gcgggacgcc tcgtaggtct
     6361 ggaagccctg gaggcgcgcc gtgatcgtgt agacgccggg gctgacgcgc gggaagcggt
     6421 agtcgccccg agcgtccgtg acggagacga gcgtgccgct gggccccttg atctcgaccg
     6481 acacgccggg gagcgcctcg cccccgtcgg ccgtgatgcg gccggtgagg acgcccgtct
     6541 gctcctgggc gcctgccggg atggcgatca ccgccaccat caacaagacg aaaaccagcc
     6601 cgagtctctt catcgttcct tcctcccttt gtagatggct cctgcctgga gccgaatgtg
     6661 gaatggacct gccgggcccg ctctcaggcc cggccttcat cgcgtgatcg agcgcacagg
     6721 agcgacggcc gcccggagcg gtgctcctcg tctggtaccc ggacctcacc ccccacatac
     6781 ggaccgcctt tcacgagccg agagtggatc gtcgccccgg accttcggtg cccccgtcgg
     6841 gaaccgatct cagattggaa ttcgatcagc gggatgttag cacgccggcc gtcaagggca
     6901 ccggaccgat gcgagggctg cccggccgcg ggcggcgggc gacgccccgc gccggacctc
     6961 accggaggcc ccgtcgcgcg gcggtttccc gaatccggcg cccatcggct atcttcccgc
     7021 cccgttcgag accacgagcc gaggaggaat ccgtgatgcg cgcgctctcc gtctgcctct
     7081 ccctcgccgc cctcgccgcc ctggccggcg tgacgctccc cgccgcggcc gccgtgaaga
     7141 ccgcgaccgt cgagtacctc gacggcgacc agacctgcga gggcttcctc gcctgggacg
     7201 acgcggtgaa ggggaagcgt cccggcgtcg tcgtcgtcca cgagtggatg gggctcggcg
     7261 actacgcgaa ggagcgggcc cgccagctcg ccgcgctcgg gtacgtggcc ctcgccgcgg
     7321 acatctacgg gaaggggatc cgcgcgaagg acgccgcgga ggcgggccgg ctcgccggcc
     7381 ggttcaaggg agacctgcct ctccttcgtt cgcgggcccg ggccgcgctc gacacgctgg
     7441 cgaaggaccc ccgcacggat gcgtcgcgcc tcttcgcgat cggctactgc ttcggcggca
     7501 cgaccgccct cgagctggcc cggagcggcg ctccggtggc gggcgtcgtc tcgttccacg
     7561 gcggcctcgc gacgaaggac cccgccgacg cgaagaacgt gcaggggcgc gtcctcgtcc
     7621 tccacggcgc cgccgacccg tacgtgccgc cggccgaggt cgcggccttc cagaaggaga
     7681 tggacgacgc gaaggtcgac tggcagatgg tcttctacgc gggcgcggtc cactccttca
     7741 cgaacccggc ggcgggaaac gactcgtcga agggggccgc ctacgacgcc cgggccgacc
     7801 gccgctcctg ggaggcgatg aaggcgttcc tcgcagaagg ggcgaagtag cccgaacgga
     7861 cgaggcgcgg cgtcccccgg cgggctacga tcctgccgga gaagccatga cgccgcgcct
     7921 tcgcaccgcc gccctcctcc ttccgctcct cgccctcctt gccctcccgg gaacggcctc
     7981 cgccgacgaa gggcagtggc cgccggaggc tctcgccacc ctcggggagg cccgctgggc
     8041 cgagctcgcc gcgcggggcc tcgagctcgg cgcgacggac ctctgggacg gaaagggggg
     8101 agggctcctg acggccgtcc tcggcctcgg cggctgcacg ggagcgttcg tctcggagga
     8161 aggactcttc ctgacgaacc accactgcgc gttcggcgcg atccagctcg cctcgacccc
     8221 ggagaagaac tacctccgcg acgggttcgt cgcccggagc cgcgcggagg aggtcccggc
     8281 gcgtggcggc gtcgggcggg tgaggatcct gacccgactc tccgacgtga ccgaccgggt
     8341 ccgcgggccg aagagcgcct tcgcacgcgc gaaggacgac gcggcacgcg aggcggcggt
     8401 cgagaaggcc cggagcgaga tcgtggccga gtgcgagaag cagcccgacc ggcgctgcgc
     8461 cgtggcggcg ttcgaggacg gccggacgta ccgcctcatg gagcagatcg agttccggga
     8521 cgtacggctc gtctacgccc cgccgcgcgg cgtcggcgac ttcggcggcg aggtcgacaa
     8581 cttccgctgg ccgcggcacg cgggagactt ctccctcctg cgcgtctggg ccgcccccga
     8641 cggatcgccg gccgctcacg cgccggagaa cgtgccctac cggccgaagc gctggctccc
     8701 ggtctcttcg aagggcgttt ccgaggggga cgtcgtgatg atcgccggct atcccggccg
     8761 gacgcagcgc tacctgaccc cctccgccgt cgagaacctc gagagctggt tctacccgct
     8821 ccgctcacgg acgttcaccg acctgatcga gatcctggag acggaaggga agaaggaccc
     8881 cgacaccgcc ctgcgcgtgt cgggcgccgt caagggctac gggaacggcg agacgaacgc
     8941 ccgcggccag gtcgaagggc tcgcccgcaa cggcgtcgcc gcccgcgccc gggaggaggc
     9001 gaaggagctc gaggagttcc tcgccgcccg gaaggacctc ccggcggagt ggaccgccgc
     9061 gccggccgag ctcgagaagg tcctcgcgcg tgaccgcgag gggcaggagc gccgtttctt
     9121 cctcgaggag atcgagcgcc tctcgtcgta cctctcctcg gccctctccg ccgtccgccg
     9181 cgccgacgag cggaagaagc cggacctcga gcgcgagccg ggcttccagg agcgtgacct
     9241 cgagagggcc cggcagcagg agaaggaccg gaccctctcg ctcgcgccgg cggcgacccg
     9301 aaaggctctc gcgtacctcg tcgagaaggc ccgggaggcc tcgaaggacg ctccggtccg
     9361 ggcgttcgac gaggcgttcg ggaaggacgc cggccgggcc gcgatcgaga agcggctcgc
     9421 ggagatggac gcggcgacct cgctctccga cgagaacgtg cggctcgcga acgtcgacgc
     9481 gacgctcgcc tccctctccg cctccgccga cccttacgtg aagctcgccg tcgggctgca
     9541 cgccgacctc gccgcgatcc ggcagaagcg aaaggagacg aacggggcgc tcctcgtctg
     9601 gcgcccgcgc tacctctcgg cgctcgaggc gctccgccga tcgaagggga aggcgatctc
     9661 ccccgacgcg aactcgacgc tgcgcgtctc gttcgccacg gtgaagggct actcgccgcg
     9721 cgaggcggtg acgttcacgc cgcggacgag cctcgccggg gtcctcgaga aggcccgcgc
     9781 cgaggagccg ttcgccgtgc ccgggaaggt cctcgacgcc gcgcgcgccg gccgcttcgg
     9841 ccggtgggcc gacccggtgc tcggcagcgt cccggtcggc ttcctgacgg acggcgacac
     9901 gaccggcggc aactcgggaa gcccgacgat gaacgggaag ggcgaggtcg tcggcctcaa
     9961 cttcgaccgc gtctgggaga acgtcgccgg ggacttcggc tggcggccgg agcgctcccg
    10021 gaacgtgaac gtcgacctgc gctacgcgct ctggatgatg gaggaggtgg acggcgcggc
    10081 ccacctcgtg aaggagctcc tgccggcgaa gtgacgaccg cgaaaggctc cgtcgaacga
    10141 cgattccggg cacgaggctg ccgggcgcgg gtcggggtcc ctctgtgcgt cgccatcctg
    10201 ctggccgcgg cgtgccggcg ggacgccccg ccgcgcaccg tggaggtcct cgtcccgttc
    10261 ctcccggcgt ccctcgaccc ctactccgac ccgcggctcg tctcgcggag catcttctcc
    10321 gcgatctacg agccgctggt cgccgagaac gacgtcggcg tccgaccggc cctggccgag
    10381 agctggacga accccgcgcc cgacacctgg cttttccgga tcgcgcccga cgcgacgttc
    10441 cacgaccgga gccccgtgac gagccgcgag gtcgtcgccg ctgcgcacgc ctcgaggacc
    10501 gcgcgcggct cggtcgcctc gctcgccgac ctccgcgcga tcgaggtcgc cgacgcccgt
    10561 acggttcgct tccggacctt ccaggccccg gaggagtttc tcctctccgt ctcggcgctc
    10621 ttcatcacac ggagggacgc ggaccgattc gtcggaagcg gcgcctaccg cgtcgtcgcc
    10681 cacacaccgg accggatcgt cctcacgcgc cacgatcggc
//
LOCUS       DAOTEZ010000104        10692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4361_length_10692_cov_6.583905, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000104 DAOTEZ010000000
VERSION     DAOTEZ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10692
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4361_length_10692_cov_6.583905"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="P5164_07305"
     CDS             <1..1055
                     /locus_tag="P5164_07305"
                     /inference="COORDINATES: protein motif:HMM:NF013717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HRY43739.1"
                     /translation="LINQHANVNRTARILADESKVELLVVQDNFLTPTARFADVVLPA
                     CTQFETWGVEDGWKYGDEVILMPKVVEPPGECRSDYRICADLATRLGVEAAFTEGRDE
                     LAWVEWVLDRYREGRFPNLPPLEELLERNVGVWAKPATRPAVAFEEFRRDPEAHPLST
                     PSGKIEIFSKALHEKGRPGEIPAVPKYVEEEESPFGPAAEAYPLQVIGHHTLHRVHST
                     HDNVDWLEEAFPQRVFLNPLDAKARGIEDGDEVRVFNARGAVRLRCRVTPRILPGVID
                     IPQGAWWTPDERGEDRRGNVNVLTSERWTPLAFGTAQHTAMAQVERVGETDAKRGTGR
                     RPRPAAAAFRRLEGTR"
     gene            1052..1669
                     /gene="dmsB"
                     /locus_tag="P5164_07310"
     CDS             1052..1669
                     /gene="dmsB"
                     /locus_tag="P5164_07310"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016443887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HRY43740.1"
                     /translation="MTGPYAVHLDASACSGCKACQAACKDHNDLPDGILWRRVYEVSG
                     GGFRRDGGAWRQDVFAWNLSLSCNHCSKPVCAEVCPTGAVRRREDGVVLLDSAKCMGC
                     RYCEWACPYGAPHFDEARGVMTKCTLCADRLEEGLAPSCVAACPMRALDFGPLGEMKE
                     RYPEARGFPLPDPGLTEPCLRMTPHPAAARSEGEGEIANLEEVER"
     gene            1666..2517
                     /locus_tag="P5164_07315"
     CDS             1666..2517
                     /locus_tag="P5164_07315"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF016838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HRY43741.1"
                     /translation="MREASLVAFTLLTQAAAGTCGVLLALELRAERAGTSSALDTVAL
                     PLLAAALLAALLGLGASFLHLGSPRNAWRALRNVRTSWLSREVLLASLFTGALAAAVL
                     LRLREGTPCFAAGFAEGFAGVVGVGLVMTMAGAYRLRTISAWDRAATPLAFFGSTLVL
                     GMLAVAAALALQSPDAASGDCASRLLLGALAVLLAGLGATLFWLRRLGRERGAARETF
                     ERTAGESRRLVAARIVLTALAAGAGLAALAGPLAAWLVVAALVLAAAAETAGRVLFYE
                     ARVRSGL"
     gene            complement(2487..4022)
                     /locus_tag="P5164_07320"
     CDS             complement(2487..4022)
                     /locus_tag="P5164_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY43742.1"
                     /translation="MESGPPAAAVPEREDRLTTPLILLTGATGYVGGRLLPSLEETGH
                     RVRCLARRPAHLAGRVGPGTEIVEGDCLRPESLPPAMEGIDTAYYLVHSMGGAGDFAE
                     QDRAAAAAFGRAAREAGVKRIVYLGGLGDRAAGLSPHLASRQETGDLLRASGVPVIEL
                     RASIVLGSGSLSFEMIRALVERLPVLLCPRWVKAEAQPIAIEDLVAYLVGALDLPAEP
                     RVFEIGGADRVSYAGIMKEYARQKGLRRLFLFVPLLTPRLSSLWLGLTTPLYARVGRK
                     LIESVRNATVVTDDAALRAFPVRPMGLAEAIERAMRNEERDFAQTRWSDALSSGTVLR
                     TRAGVKLGSRLVDSRTAFVAAPPAAAFAPIRRIGGARGWYYATWLWKIRGFLDLLAGG
                     VGMRRGRPHPEEVRVGDALDFWRVEAFEPDRRLRLAAEMKVPGRAWLELEVTPAEGGS
                     TIRQTAVFDPAGLPGLAYWYGIYPIHARVFAGMLAALAREATTGDDLRPSASQSPERT
                     RAS"
     gene            4115..4522
                     /locus_tag="P5164_07325"
     CDS             4115..4522
                     /locus_tag="P5164_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019028297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRY43743.1"
                     /translation="MLNRLLAPRAEAAYALLRVVSGLLFALHGAQKLFGVLGGTVQAA
                     GSQLWVGAVIELVAGLAIAAGVFTSWAAFLASGTMAVAYVQFHWKLAFDAGFFPVVNK
                     GELALLYSVLFLFVACRGGGPYGWDGRKGSKES"
     gene            complement(4438..5619)
                     /locus_tag="P5164_07330"
     CDS             complement(4438..5619)
                     /locus_tag="P5164_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRY43744.1"
                     /translation="MALRLLFLSDTHLGLDLPGRPRAERPRRGDDLFASFERALEPAV
                     RGEAGVVVHGGDLFYRSRVPAWLAARVFARLADLADAGVDLFWVPGNHERGAVPRELL
                     LTHPRVRVFDRPRTFLLSRDGVTLALAGFPYAPRIRDDAPALFGATGAASVPADVRLL
                     CLHQAVEGATVGPWDFVFRSGVDVLRGRDVPPGFAALLSGHIHRSQVLARDLSGRPLA
                     APVLFAGSTDRVSFVERFEPKGAFLLQLSPDGSPGGRATWAFREHPVRPMAVVDLDPA
                     PAGLSGRIDAALGRLEPRSLVRLRLLAEPPAESRTLLRPDALRARAPEGVDVSVAWPR
                     PEGAAPRTAPSPVPAARSGRSRRAAPPGDAEAQLSFEPFRPSQPYGPPPRQATKRKRT
                     E"
     gene            complement(5732..6310)
                     /gene="nuoE"
                     /locus_tag="P5164_07335"
     CDS             complement(5732..6310)
                     /gene="nuoE"
                     /locus_tag="P5164_07335"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRY43745.1"
                     /translation="MSEHAACTDNWEEVKATSLRILPPHIVEFIEKNAKTEHPESNLI
                     ATLHMVQSHFGFLGQTQMHAVAQLAQIPYATVTGVATFYHYFRLQPRGKHVINVCLGT
                     ACYVKGADKLIARLSDDLGVHLGETTKDQMFSLESARCLGTCGLAPVMMVGDQVYGPV
                     TPAEASRILDKYLKGEKAKTAPREEPAATHVS"
     gene            complement(6307..8079)
                     /locus_tag="P5164_07340"
     CDS             complement(6307..8079)
                     /locus_tag="P5164_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HRY43746.1"
                     /translation="MPTSQKMVTIKIDDETIQVPSGMKVINAAEKIGIHIPRLCYHPA
                     LSIEGACRVCVVEVKGFNHFLTACSQDVWEGMEVQTNSPEIRQARRDIVELLLDNHPM
                     DCQTCDRDGNCELQKQAYRLGIRERLFAGKRKQLPVEDSGQSVVRTPEKCILCGRCVR
                     VCSEVQGVSNLSQHGRGFHTVVGPANLANMDDSVCIQCGQCVAVCPTAAFLEKDHTER
                     VWEALALPRDVKHVVVHTAPAIRATIGECFGMPVGTPVTGKLVTALRRMGFDGVFDTN
                     FGADLTIIEEANEFLGRFQRDENLPLITSCSPGWVNFLEKFYPEMIPHTSTCKSPMQM
                     TSTLLKTYYAAKKGLDPKDVYVVSIMPCVAKKFEAARPEHRMPDGTPHTDAVLTTREL
                     AWMIKSYGIDFAALPDGDFDRPLGISSGAADIFGATGGVMEAALRTAAVKLTGKELGP
                     LVFEEVRGVKGLKEATVHINGLDVNIAVSNGLTNAKAILDAAKAGGKKYHLIEIMACP
                     GGCIMGGGQPYPPLDEDPMDPEIAKLRARALYDIDSHKQLRRSHENPAIEHLYEEFLG
                     EPNGKKAHELLHTHYQARMPRGIR"
     gene            complement(8096..10273)
                     /locus_tag="P5164_07345"
     CDS             complement(8096..10273)
                     /locus_tag="P5164_07345"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRY43747.1"
                     /translation="MPDTVTQAFQSLAARSEEALALRGDAGKIVVQIGSATCEHAAGS
                     REVADEFVKHLASSGREDVVLHRTGCTGRCSKEPIVGVSVPGKPPVKYERVDRELVHK
                     IFTSHIQKGAALPEHALDGSLEKPYEYELVFCEGRRCKQRTDLKASFEAKLAAAGIGS
                     DRVKVNSHACLGVCSEETEGKSTHVLVRPSKTIYRLENEADLDALLEGPVRKGTIDER
                     MKVPFKPIAQDFFDLYADVRFFNAQSRIAMRNAGILDPESLEEYVRFGGFQALAKCLE
                     KGDPDWIVDEVLKAKLRGRGGGGFLTGKKWQFARANEEKTRYIICNGDEGDPGAFMDR
                     GMLESDPFNVVEGMIIGAYAIGACKGFYYIRAEYPLAIKRIENALQKCREAGILGENV
                     MGSGWSFDMEIRLGAGAFVCGEETALIRSIEGERGQPRVRPPFPTTAGLWGKPTCINN
                     VETFANVTAIINFGGDWFAQVGTEASGGTKVFALAGKVKHTGLVEVPLGTPLKDVVFD
                     IGGGVLDDRELKAIQTGGPAGGFIPANRADMPVDFDPLAKAGSIMGSGGMIVLSEEDC
                     MVDIAKFYLTFTQEESCGKCTPCREGTTRMLEILEKITAGNGELSDLDRLERLGKLCQ
                     RTSLCGLGRAAPNPVLSSLKHFRDEYREHIVDKKCRAKKCTALIQYVIDDEKCIGCTA
                     CARNCPTQCISGERKKAHVIDQARCIKCGQCYEICRFDAVERV"
BASE COUNT         1415 a         3615 c         4054 g         1608 t
ORIGIN      
        1 cgctgatcaa ccagcacgcg aacgtgaacc ggacggcgcg gatcctcgcc gacgagtcga
       61 aggtggagct cctcgtcgtc caggacaact tcctgacgcc gacggcccgg ttcgccgacg
      121 tcgttctccc cgcctgcacg cagttcgaga cgtggggcgt cgaggacggg tggaagtacg
      181 gcgacgaagt gatcctcatg ccgaaggtcg tcgagcctcc cggcgagtgc cggagcgact
      241 accggatctg cgccgacctc gcgacgcgtc tcggcgtgga ggcggccttc acggaagggc
      301 gcgacgagct tgcctgggtg gagtgggtcc tcgaccgcta ccgcgagggc cgattcccga
      361 acctgccgcc tctcgaggag ctcctcgagc ggaacgtcgg cgtctgggcg aagccggcga
      421 cgcgccctgc ggtggcgttc gaggagttcc ggagggaccc cgaggcgcat cccctctcga
      481 cgccctcggg gaagatcgag atcttctcga aggccctcca cgagaagggg aggcccggcg
      541 agatcccggc cgtgccgaag tacgtcgagg aggaggagag cccgttcggc ccggcggcgg
      601 aggcgtaccc gctgcaggtg atcggccacc acacgctcca ccgggtccac tcgacgcacg
      661 acaacgtcga ctggctggag gaggcgttcc cgcagcgcgt cttcctgaac ccgctcgacg
      721 cgaaggcgcg cgggatcgag gacggcgacg aggtccgcgt cttcaacgcc cgcggcgccg
      781 tccgcctccg ctgccgggtc acgccgcgga tcctcccggg ggtgatcgac atcccgcagg
      841 gagcgtggtg gacgcccgac gagcggggcg aggaccgccg cggcaacgtg aacgtcctga
      901 cgagcgagcg ctggacgccg ctcgccttcg ggacggcgca gcacacggcg atggcgcagg
      961 tggagcgggt cggggagacg gacgcgaagc gcggaacggg gcgacgcccg cgccctgcgg
     1021 ccgcggcctt ccggcgcctg gaggggacgc ggtgacgggg ccgtacgccg tccacctcga
     1081 cgcctcggcc tgcagcggct gcaaggcctg ccaggcggcc tgcaaggacc acaacgacct
     1141 ccccgacggg atcctctggc ggcgcgtcta cgaggtctcg ggcggcggct tccggaggga
     1201 cggcggcgca tggcgccagg acgtcttcgc ctggaacctc tccctctcct gcaaccactg
     1261 ctcgaagccc gtctgcgccg aagtctgccc gacgggggcc gtgcggcggc gggaggacgg
     1321 cgtcgtcctc ctcgactccg cgaagtgcat gggctgccgc tactgcgagt gggcctgccc
     1381 gtacggcgcg ccgcacttcg acgaggcgcg cggcgtgatg acgaagtgca cgctctgcgc
     1441 cgaccgcctc gaggaggggc tcgcgccctc ctgcgtcgcc gcctgcccga tgcgggcgct
     1501 cgacttcggt ccgctcggcg agatgaagga gcgatacccg gaggcgcgcg gcttcccgct
     1561 tcccgacccc ggcctgacgg agccgtgcct tcggatgact ccccatccgg ccgcggcgcg
     1621 ctcggaaggg gaaggggaga tcgcgaacct ggaggaggtg gagcggtgag ggaagcctcg
     1681 ctcgtcgcct tcacgctcct gacgcaggcg gccgccggga cctgcggggt cctcctcgcg
     1741 ctcgagctcc gggccgagcg ggccggaacg tcctcggcgc tcgacaccgt ggctcttccg
     1801 ctcctcgccg cggcgctcct cgccgcgctg ctcggcctcg gcgcctcgtt cctccacctc
     1861 ggcagcccgc gcaacgcgtg gcgggcgctc cggaacgtcc gcacgtcgtg gctgagccgc
     1921 gaggtcctcc tcgcgtcgct cttcacgggc gcgctcgcgg cggcggtcct cctgcggctc
     1981 cgcgagggga cgccgtgctt cgcagccggc ttcgcggagg ggttcgccgg cgtcgtcggg
     2041 gtcggcctcg tgatgacgat ggccggggcc tatcggctcc ggacgatctc cgcgtgggat
     2101 cgggcggcga cgccgctcgc cttcttcggc tcgacgctcg tcctcggcat gctcgccgtc
     2161 gccgcggccc tcgcgctgca gtcgcccgac gcggcgtcag gcgactgcgc ctcacgactc
     2221 ctcctcggcg ctctcgccgt cctcctcgcg gggctcggcg cgacgctttt ctggctgcga
     2281 cggctcggcc gggagagggg agcggcgcgc gagacgttcg agaggacggc gggggagagc
     2341 cgccggctcg tcgcggcgcg gatcgtcctg acggccctcg cggccggcgc cggcctcgcc
     2401 gcgctcgcgg gacctctggc cgcctggctc gtcgtggcgg cgctcgtcct cgccgcagcg
     2461 gccgagacgg cgggacgcgt cctcttctac gaggcgcgcg tccgctccgg gctctgagag
     2521 gccgacggac gcaggtcgtc gcccgtcgtc gcctcccgcg cgagggcggc gagcatcccg
     2581 gcgaagacgc gggcgtggat cggatagatg ccgtaccagt aggcgaggcc cgggaggccc
     2641 gcggggtcga agacggcggt ctgccggatc gtcgagcccc cctcggccgg cgtcacctcg
     2701 agctcgagcc aggcgcggcc ggggaccttc atctcggccg cgaggcggag ccgccggtcg
     2761 ggctcgaacg cttcgacgcg ccagaagtcg agcgcgtcgc cgacccggac ctcctcgggg
     2821 tgcgggcgtc cgcggcgcat cccgacgccg ccggcgagga ggtcgaggaa gccgcggatc
     2881 ttccagagcc aggtcgcgta gtaccagccc cgcgcgccgc cgatgcgccg gatcggcgcg
     2941 aacgccgcgg cgggcggcgc cgcgacgaag gcggtgcgcg aatcgacgag gcgcgagccg
     3001 agcttcaccc cggcccgcgt ccggagcacg gtgccggagg agagggcgtc ggaccagcgg
     3061 gtctgcgcga agtcgcgttc ctcgttccgc atcgcccgct cgatcgcctc cgcgaggccc
     3121 atcgggcgga ccgggaaggc gcggagggcg gcgtcgtcgg tgacgacggt ggcgttcctc
     3181 acgctctcga tgagcttccg gccgacgcgg gcgtagagcg gcgtcgtcag gccgagccag
     3241 aggctcgaga ggcggggcgt caggagcggg acgaagagga agagccgccg gaggcccttc
     3301 tgccgcgcgt attccttcat gatgccggcg tacgagaccc tgtcggcgcc gccgatctcg
     3361 aagacgcgcg gctccgcggg gaggtcgagc gccccgacga gataggcgac gaggtcctcg
     3421 atcgcgatcg gctgcgcctc ggccttcacc cagcgcgggc agaggaggac ggggagcctc
     3481 tcgacgagcg cccggatcat ctcgaaggag aggctcccgg agccgaggac gatcgaggcg
     3541 cgcagctcga tcaccgggac gcccgacgcg cggaggaggt cgcccgtctc ctgccggctc
     3601 gcgaggtgcg gggagaggcc ggcggcgcgg tctccgagac cgcccaggta gacgatccgc
     3661 ttcacgcccg cctcgcgcgc ggcccggccg aaggcggccg ccgcggcgcg gtcctgctcg
     3721 gcgaagtcgc ccgcccctcc catcgagtgg acgaggtagt aagcggtgtc gatcccctcc
     3781 atcgcgggag gaagactctc cggccggagg cagtcgcctt cgacgatctc ggtgccgggg
     3841 ccgacgcgcc cggcgaggtg agccggacgc cgggcgaggc agcgtacgcg gtgcccggtc
     3901 tcctccaggg acgggaggag acggcccccg acatagccgg tggctccggt caggaggatg
     3961 aggggtgtgg tcaggcgatc ctcgcgttcc ggaaccgccg cggcgggcgg tcccgactcc
     4021 aatccgacgc ggattctccg ccggatctcg ggcgtacggg caggtgcgat gatcccggcg
     4081 ttccggccag gtcaggcacg aatggaggag tctcatgctg aatcgtcttc tcgctccgcg
     4141 ggcagaggcc gcctatgccc ttctccgagt cgtgtcgggg ctcctgttcg ccctgcacgg
     4201 cgcccagaag ctcttcggcg tcctcggggg gacggtccag gcggccggct cgcagctctg
     4261 ggtcggggcc gtcatcgagc tcgtggccgg cctcgcgatc gcggccggcg tcttcacgag
     4321 ctgggccgcg ttcctcgcga gcgggacgat ggcggtggcc tacgtccagt tccactggaa
     4381 gctggcgttc gacgccggct tcttcccggt ggtgaacaag ggcgagctcg cgctcctcta
     4441 ctccgtcctc ttcctcttcg tcgcctgtcg cggcggcggc ccgtacggct gggacggccg
     4501 gaagggctcg aaggagagct gagcctcggc gtccccgggc ggtgcggcgc gccgggagcg
     4561 gccggagcgc gccgcgggaa cgggggaggg cgccgtgcgg ggcgcggctc cttccggacg
     4621 cggccacgcc acggagacgt cgacgccctc cggagcccgc gcccggaggg cgtccggccg
     4681 gaggagcgtc cgcgattccg cgggcggctc ggcgaggagg cggagccgga cgaggctccg
     4741 cggctcgagc cggccgagcg ccgcgtcgat ccgcccggag aggccggcgg gcgccgggtc
     4801 gaggtcgacg acggccatcg gccggaccgg gtgctcgcgg aaggcccacg tcgcgcggcc
     4861 gccgggggag ccgtccggcg agagctggag gaggaatgcc cccttcggct cgaagcgctc
     4921 gacgaaggag acgcggtcgg tcgagccggc gaagaggacc ggcgccgcga gggggcgacc
     4981 ggagaggtcc ctcgcgagga cctgcgatcg gtggatgtgc cccgagagga gcgcggcgaa
     5041 gccgggcggg acgtcgcggc cgcggaggac gtcgaccccc gagcggaaga cgaagtccca
     5101 cgggccgacg gtcgccccct cgactgcctg gtgcaggcag aggagccgga cgtccgccgg
     5161 aacggacgcg gcgcccgtgg cgccgaagag ggcgggggcg tcgtcgcgga ttcgcggggc
     5221 gtacgggaag ccggcgaggg cgagcgtgac gccgtcgcgc gagaggagga aggtccgcgg
     5281 gcggtcgaag accctcacgc gcgggtgcgt gaggaggagc tcgcgcggga ccgcgccgcg
     5341 ctcgtggttg ccggggaccc agaagaggtc gacgccggcg tcggcgaggt ccgcgaggcg
     5401 ggcgaagacc cgcgcggcga gccacgccgg gacccggctc cggtagaaga ggtcgccgcc
     5461 gtggacgacg acgcccgcct cgccgcgcac ggccggctcg agcgcccgct cgaagctcgc
     5521 gaacaggtcg tcgccgcggc gcggtcgctc cgcccgcggc cggccgggga ggtcgaggcc
     5581 gaggtgcgtg tcggagagga agaggaggcg cagggccacg tgccgaggct accccgaccc
     5641 gaaaagcgaa acggggccgg aaggccccgt tcggtacgaa gggagagcgg cgggcggggc
     5701 cggccggagc cggccccgcc gccgcgcgag gtcaggagac gtgcgtggcg gccggctcct
     5761 cgcggggagc cgtcttcgcc ttctctccct tgaggtactt gtcgaggatc cggctcgcct
     5821 ccgcgggcgt gaccgggccg tagacctgat cgccgaccat catgacggga gcgaggccgc
     5881 aggtgccgag gcagcgggcg ctctcgagcg agaacatctg gtctttcgtc gtctcgccga
     5941 ggtggacgcc caggtcgtcg gagagccgcg cgatgagctt gtcggccccc ttcacgtagc
     6001 acgccgttcc gaggcagacg ttgatgacgt gcttgccgcg cggctggagg cggaagtagt
     6061 ggtagaaggt cgcgacgccg gtgaccgtcg cgtacgggat ctgcgcgagc tgggccacgg
     6121 cgtgcatctg cgtctggccc aggaacccga agtgggactg gaccatgtgg agcgtcgcga
     6181 tgaggttgct ctcgggatgc tcggtcttcg cgttcttctc gatgaactcg acgatgtgcg
     6241 gcggcaggat gcggaggctg gttgccttca cttcctccca gttgtcggtg caggcggcgt
     6301 gttcgctcac cggatccccc tcggcatgcg ggcctggtag tgggtgtgga gcagctcgtg
     6361 cgccttcttc ccgttcggct cgccgaggaa ctcctcgtag aggtgctcga tggcggggtt
     6421 ctcgtgggag cggcggagct gcttgtggga gtcgatgtcg tagagggcgc gggcgcggag
     6481 cttcgcgatc tccgggtcca tcgggtcctc gtcgaggggc gggtagggct ggccgccgcc
     6541 catgatgcag ccgcccggac aggccatgat ctcgatgagg tggtacttct tcccgccggc
     6601 cttggcggcg tcgaggatcg ccttcgcgtt cgtcaggccg ttcgagacgg cgatgttcac
     6661 gtcgaggccg ttgatgtgga ccgtggcctc cttcaggccc ttgacgcccc ggacctcctc
     6721 gaagacgagc gggccgagct ccttgccggt cagcttcacg gcggcggtgc ggagcgccgc
     6781 ctccatgacg ccgcccgtgg cgccgaagat atcggcggcg ccggagctga tcccgagcgg
     6841 gcggtcgaag tcgccgtccg gaagggcggc gaagtcgatg ccgtaggact tgatcatcca
     6901 ggcgagctcg cgggtcgtca ggacggcgtc ggtgtgcggg gtgccgtcgg gcatccggtg
     6961 ctcggggcgc gccgcctcga acttcttggc gacgcacggc atgatcgaga cgacgtagac
     7021 gtccttcggg tcgagcccct tcttcgcggc gtagtacgtc ttcaggagcg tgctcgtcat
     7081 ctgcatcggc gacttgcacg tcgaggtgtg ggggatcatc tcggggtaga acttctcgag
     7141 gaagttgacc cacccggggc tgcagctcgt gatgagcggg aggttctcgt cgcgctggaa
     7201 gcggccgagg aactcgttgg cctcctcgat gatcgtcagg tcggcgccga agttcgtgtc
     7261 gaagacgccg tcgaagccca tccggcggag ggccgtgacg agcttgccgg tcacgggcgt
     7321 cccgaccggc atgccgaagc actcgccgat cgtggcgcgg atggcggggg cggtgtggac
     7381 gacgacgtgc ttgacgtcgc gcgggagggc gagcgcctcc cagacgcgct cggtgtggtc
     7441 cttctcgagg aaggcggccg tcgggcagac cgcgacgcac tggccgcact ggatgcagac
     7501 cgagtcgtcc atgttcgcga ggttcgccgg gccgacgacg gtgtggaagc cgcgcccgtg
     7561 ctgggagagg ttgctgacgc cctggacctc ggagcagacg cggacgcagc ggccgcagag
     7621 gatgcacttc tcgggcgtcc tcacgacgct ctgtcccgag tcctccaccg ggagctgctt
     7681 gcgcttgccg gcgaagagcc tctcgcggat gccgaggcgg taggcctgct tctggagctc
     7741 gcagttcccg tcccggtcgc acgtctggca gtccatcggg tggttgtcga ggaggagctc
     7801 gacgatgtcg cgccgcgcct ggcggatctc cgggctgttc gtctggacct ccatcccctc
     7861 ccagacgtcc tgcgagcagg cggtcaggaa gtgattgaag cccttcacct cgacgacgca
     7921 gacgcggcag gcgccctcga tcgagagcgc ggggtggtag cagaggcggg ggatgtggat
     7981 gccgatcttc tcggcggcgt tgatgacctt catccccgag gggacctgga tcgtctcgtc
     8041 gtcgatcttg atcgtgacca tcttctggct cgtgggcata tcggccgtcc ttcgctcaga
     8101 cccgctcgac cgcgtcgaac cggcagatct cgtagcactg gccgcacttg atgcagcggg
     8161 cctggtcgat cacgtgggcc ttcttcctct cgcccgagat gcactgcgtc gggcagttgc
     8221 gggcgcaggc ggtgcagccg atgcacttct cgtcgtcgat gacgtactgg atgagggcgg
     8281 tgcacttctt ggcgcggcac ttcttgtcga cgatgtgctc gcggtactcg tcgcggaagt
     8341 gcttcagcga ggagaggacc gggttcggcg cggcgcggcc gaggccgcag aggctcgtcc
     8401 gctggcagag cttcccgagc cgctcgagcc ggtcgaggtc ggagagctcg ccgttgccgg
     8461 cggtgatctt ctcgaggatc tcgagcatcc gggtcgtccc ctcgcgacag ggggtgcact
     8521 tcccgcagct ctcctcctgg gtgaacgtca ggtagaactt cgcgatgtcg accatgcagt
     8581 cctcttcgga cagcacgatc attccgcccg atcccatgat cgagccggcc ttcgcgagcg
     8641 ggtcgaagtc gacgggcatg tcggcgcggt tcgccgggat gaagccgccc gcggggccgc
     8701 ccgtctggat cgccttcagc tcgcggtcgt ccaggactcc gccgccgatg tcgaagacga
     8761 cgtccttgag cggcgtgccg agcgggacct cgacgaggcc cgtgtgcttg accttgccgg
     8821 cgagggcgaa gaccttcgtc ccgccggagg cctcggtccc gacctgcgcg aaccagtcgc
     8881 cgccgaagtt gatgatggcg gtgacgttcg cgaaggtctc gacgttgttg atgcaggtcg
     8941 gcttgcccca gaggccggcc gtcgtcggga acggggggcg gacgcgcggc tggccgcgct
     9001 cgccctcgat cgagcggatg agggccgtct cctcgccgca gacgaaggcg cccgcgccga
     9061 gccggatctc catgtcgaag ctccagccgc tccccatgac gttctcgccg aggatccccg
     9121 cctcgcggca cttctgcagg gcgttctcga tccgcttgat ggcgagcggg tactcggccc
     9181 ggatgtagta gaagcccttg caggcgccga tcgcgtaggc gccgatgatc atcccctcga
     9241 cgacgttgaa cgggtcgctc tcgagcatcc cgcggtccat gaaggccccg gggtcgccct
     9301 cgtcgccgtt gcagatgatg tagcgggtct tctcctcgtt cgcgcgggcg aactgccact
     9361 tcttgccggt gaggaagcct cccccccctc ggccgcgcag cttcgccttc aggacctcgt
     9421 cgacgatcca gtccggatcg cccttctcga ggcatttcgc gagcgcctgg aagccgccga
     9481 agcggacgta ctcctcgagg ctctcggggt cgaggatccc ggcgttgcgc atcgcgatcc
     9541 ggctctgcgc gttgaagaac ctcacgtcgg cgtagaggtc gaagaagtcc tgggcgatcg
     9601 gcttgaaggg gaccttcatc cgctcgtcga tcgtcccctt ccggacgggg ccttcgagga
     9661 gcgcgtcgag gtcggcctcg ttctcgagcc tgtagatcgt cttcgagggc cggacgagga
     9721 cgtgcgtcga cttcccctcg gtctcctcgg agcagacgcc gaggcaggcg tgcgagttga
     9781 ccttgacgcg gtcggacccg atgccggcgg cggcgagctt cgcctcgaag gaggccttca
     9841 ggtccgtccg ctgcttgcag cggcgcccct cgcagaagac gagctcgtac tcgtacggct
     9901 tctcgagcga cccgtcgagc gcgtgctcgg ggagggcggc gcccttctgg atgtggctcg
     9961 tgaagatctt gtggacgagc tcgcggtcga cgcgctcgta cttgacgggg ggcttcccgg
    10021 ggaccgaaac gccgacgatc ggctccttgc tgcagcggcc ggtgcagccg gtccggtgga
    10081 ggacgacgtc ctcgcgcccc gaggaagcga ggtgcttgac gaactcgtcc gccacctcgc
    10141 gcgagccggc ggcgtgctcg caggtggcgg aaccgatctg cacgacgatc ttcccggcgt
    10201 cgccgcgcag ggcgagcgcc tcctcggacc gcgccgccag tgattgaaag gcttgggtca
    10261 cggtgtctgg catttgggct ccatagaagg ctcggggaag ctcctggccc cccgcgagcg
    10321 gaagcgggac gtttcgaaag gacgcagcgc gacggcacgg cctgccctcc ggggttccgt
    10381 cgtcggctgc gggaatcggg accgatttgg gtgggattgg gtcgaacggg agaggccggc
    10441 acggcgtcgg agtcgccgcg cgcggtccat caggcgccgc ctgctccggt tcggagggaa
    10501 tgttcgtggc cgggatcggt gcggcgcgag gagctccggc tccggggaac gccggagtcg
    10561 acgcgtcggg cgcggtcgat ccggagtccg gtcctggggc ggtccttcgg cgtgctcatg
    10621 atcctgggcg cgcccgatgc cttgccgggc gcgagggggc tccgtttccg gtgcctgccc
    10681 ccgtttcggg cc
//
LOCUS       DAOTEZ010000105        10537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4428_length_10537_cov_6.477104, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000105 DAOTEZ010000000
VERSION     DAOTEZ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10537
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4428_length_10537_cov_6.477104"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1528
                     /locus_tag="P5164_07350"
     CDS             <1..1528
                     /locus_tag="P5164_07350"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRY43748.1"
                     /translation="AEEFLLRGRAIAIAGDDRVNESVTRKFLARTWRTLGQTGRALPE
                     AEAALALARQIGNRFEVKNVLLELAAIHEAGGRPDQGYRHLKDGLTLLEEIQGAEVHR
                     KVAAVQEQLEIEKREGQIRLLRKEQELGELAFRNQRTVRNFLIALLAVALAAGAGAYR
                     AYAQKKRAAETIGRQNAELLAIDRIARDINKEVELRSLIESILRHSLEHFPQAARGGF
                     LALADEDSRFRSLVSFGYPEAALEKVALAEEAARARYVEAGEEVAEGLHVVRRPGSLE
                     GLGEAYRPAASLLVFVIRDESATVGYLVLESVDDPAAFDRIDVRQVKTLHEHIISAVQ
                     KARRLRQVERLSRTDPLTRLANRRHVQERLALEAERASRYGTPFLVAMCDLDDFKNVN
                     DTLGHDCGDEVLRSTARLLSENLRRTDLVGRWGGEEFLVLLPETEREGGRIALEKVRA
                     KLEALPLVYAGAPLRVTVSIGLAAFVRGERLEDLVARADAALYEAKRSGKNRVVVAGG
                     "
     gene            complement(1607..4522)
                     /locus_tag="P5164_07355"
     CDS             complement(1607..4522)
                     /locus_tag="P5164_07355"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRY43749.1"
                     /translation="MIGVKTILSRLARAYALWGALLVLLGLPGALLRIGTTRERPVRL
                     ESIPAPDGSLGGTVLADGRTTRFSAISIGGGPWAPAGGVVAARSLFDLPAGTPVAFRE
                     AGAGARVVTGAVRERRSTVDESVTRATFALLGVALALAGAGIALAGVGLQALLAGAAL
                     AGLGHFVGARFLEANASLVQSTPLREAIVLVWVSFPRHFAFPALAAFLSLFPSDLTRS
                     RACRVLLGLLGATALAQALLVPLFQVPGTLDSLGTGTQAFLLGGTRRLTSFLFGAGSL
                     GGLLLFFFQARALRMEPLSSATRRRASVIGAGLLVGLGPPLAAALAQIAWIAGTGRLL
                     LPPVAIALSLLPVLILPLVLAYAMLSPRVLSVGLLVKKAILLAFAEGSVRVVSLAPLA
                     ALGLLFWTRREVPIGEVFSSHALFICAALAATTAGLRYGDRTRSLLERLFFDARKASS
                     GTLASLAGEARRARDVEELAEAFSGGIERALVAEPSTLFVRDDRTGSFVSPGRPLPAL
                     DGTSPIVEAAALRPGPIRLDGDAAGFADLSEMDRNWLAASRARILVPLTGSTGGLLAL
                     LAVGEKASERPFDPEDEQFLSAAASAGALALENLLLRSSQASSAGPSHAGRPTDAIEA
                     PEAARLCRRCRRLFPSDAGSACPDDETFFETVPAPYLLAGKYRLERWVGAGGMGVIYK
                     ARDLALGRDVAVKTLPSLSAGSVRRLQREARAGAQLIHPSLGLIFALESWHGTPMLVL
                     EYLPGGTLADRIRSGPVAPALAAAWGAALASGLEALHARGVLHRDLKPSNVGFAADGA
                     PKLLDFGLVRLLGETDASFAPGVPPDLRGARGETDSSTRTAASHVVGTVPYLSPEALQ
                     GHPPSPGFDLWGLSLTIYEALTGVNPFAASSSTRTAERVLTLPVPDPRGYRPDCPGSL
                     AAVLLAALARDERVRPATARSLREAFEGAGRGLPSISGESPSLRARDETFPGPAPTA"
     gene            4577..5089
                     /locus_tag="P5164_07360"
     CDS             4577..5089
                     /locus_tag="P5164_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43750.1"
                     /translation="MPFDRRWLLTAAGSAFLASLSKDGLATPPTAGAKPTGDPAKDKE
                     LAFQEFRIGYGDLTADEEKVVRKALEGTYGERFDRLYPSLTEAEKRTLLGFACFLGTL
                     TSRCLENEQKGRCGWKGDPSATLREEHVQRARELLGYRLNTKLKFPCSSAVSSPNVLQ
                     ADEPCPLCPG"
     gene            complement(5039..6910)
                     /locus_tag="P5164_07365"
     CDS             complement(5039..6910)
                     /locus_tag="P5164_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43751.1"
                     /translation="MFRAQALLLALLLALAGLLGALGRERQRAAALGRAPDRAGGKVE
                     RCVTCHVRPEEDPGGAHARAAVGCSSCHLGNPFAFDKARAHAGMEPEPGALRSVARTC
                     GRSGCHVREAARVATSLMARASGIVSVNRWAFGEVPEPRGTATMVDLLAKEERTPAED
                     HLAKLCAGCHLHALRASRDDAIHGNGSGCSACHVARRLPGAVPRPHPSVDAHVTDDRC
                     LGCHSRSGRIALTYEGLYEIEPAQAAALGTAATTLHDGRPAGHAEADVHRRAGLSCVD
                     CHLHTDLMGDGTRHEHSESQVEIACEACHGPARDGGETTWAEVRDPITRDLLRQQGET
                     RPPGERVRLGRHGTPVWNLRPSEGGWSTLRKRQGVALATRPTPADANHRLPGHERLTC
                     SACHSAWAPTCSTCHTRYDAEGAQWDFAKAAETPGRWVEASEGYDARPPALAVRPDGR
                     IAPAIPGMVMDLDAAAASGPRFSRRLYASLDPHSTGKKARDCASCHLSSWALGLGTGT
                     LSFESGAASFSPASPATDDARLAVDGWTLLDAPSPGIGTRVGLRSLDAAELRRALAVS
                     ACLPCHAKATDLVWRDFAGSKARLARGGTRCGFRSAGSPRPSARAEGAGLVGLKDIR"
     gene            complement(6910..7731)
                     /locus_tag="P5164_07370"
     CDS             complement(6910..7731)
                     /locus_tag="P5164_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HRY43752.1"
                     /translation="MILGRVTFGLLAVALFSGLALAPFWSAGRALESLETLNGATPWG
                     FFLRGVHAFSAWGFLLGTLAHLGEVLWKRTESRLRPAVWWRSVALLPVAVLALLGGFL
                     MRGDAEAQAALAVWREVSSSVPIAGRALAALLLGLPGADLAPVSLHHAGTFTLLAWLL
                     TAEHGGTLLPDLRSTVLAALASFAAAGLFPVSLGPPVAPDGALLLGPWYLLGLQGALL
                     DLPVAAGWLLPLALLLAVGLVRHADGRARAALLALAAAWGAGYLALTLRLLLAAR"
     gene            complement(7728..8132)
                     /locus_tag="P5164_07375"
     CDS             complement(7728..8132)
                     /locus_tag="P5164_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HRY43753.1"
                     /translation="MSSRRDFLGTAWAAAGVAAAAAGTAVLGRALSGSPEPERGVRLP
                     AELLARAEKEGGLVHEGLHVRGPAASPVVLDLTCTHLRCRVAPVEGGGFSCPCHASRY
                     DAEGRVTAGPASRPLARPRLSPDGAGFVARLS"
     gene            complement(8129..9523)
                     /locus_tag="P5164_07380"
     CDS             complement(8129..9523)
                     /locus_tag="P5164_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRY43754.1"
                     /translation="MPDTLSAPQTSSLAPPGFGTLETVFAALGRVLVVLDREFRVIRA
                     SRSLDDLARPGAVEAALGRPIEELVGARLFGPSESLREALEQGHREEGRRAILRCGEE
                     SARLVSLTAAVLPSDVTSHCDPRARYIVVLRPAETDNGLVQSMLSSHGLVARSPAMRG
                     IVHLVESLHRSEATVLVTGESGTGKELIARALHANSPRCTGPFVAVNCGALPGDLLES
                     ELFGHVRGAFTGAVRDRVGRIDLARGGTLFLDEVGDIPPHLQVKLLRVLQERRFERVG
                     ESTPRPMDARVIAATNVDLAEAIRVGRFRDDLYYRLRVVPIHVPPLRERVEDIPLIAH
                     HLLAHIGGREGRALLLSPDTLAAMARYPWPGNVRELGNALEYAVATCTGQTIQIENLP
                     PELRQGAERSVLEPASAQDAAPLGDALPEDPAERLRILQTLESARWNRGRAAELLGMS
                     RSTLWRRMKELGIE"
     gene            9579..>10537
                     /locus_tag="P5164_07385"
     CDS             9579..>10537
                     /locus_tag="P5164_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRY43755.1"
                     /translation="MTSIYFGEEHESFRRAVRQFLEGEVAPRAREWEEARRIPRDVFR
                     RMGDLGFLGVLAPEEVGGADASIFHAIALLEELPRSRMGGFVAAVSVQQFIATGAIAL
                     HGTGEQKRRWLAPSVAGTKVGAIAISEPDTGSDVAAIRTTARRDGDAWVIDGAKTWIT
                     NGVEGDFVVVACRTAADAGAGGISLVVVEADTPGFSRSRLRKMGWHCSDTAELTFADV
                     RVPLGNLVGAENEGFFLIMETFVLERLVTAATAVGSARLALEETRRYVLSREAFGRPI
                     GKFQAIRHRLADLHAELEAASQLVYHAAWLHERGENPVAEACMA"
BASE COUNT         1522 a         3620 c         4191 g         1204 t
ORIGIN      
        1 ggcggaggag ttcctccttc ggggccgcgc gatcgcgatc gcgggagacg accgggtcaa
       61 cgagagcgtc acgcgcaagt tcctcgcgcg aacctggcgg acgctcgggc agaccggccg
      121 ggcgctcccg gaggcggagg cggcgctcgc gctcgcccgc cagatcggga atcggttcga
      181 ggtgaagaac gtcctcctcg agctcgcggc gatccacgag gcgggcggga gaccggacca
      241 gggataccgg cacctgaagg acgggctgac gctcctcgag gagatccagg gcgccgaggt
      301 ccaccggaag gtcgcggcgg tccaggagca gctcgagatc gagaagcggg aggggcagat
      361 ccgcctcctg cggaaggagc aggagctggg cgagctcgcg ttccggaacc agcggacggt
      421 tcgcaacttc ctgatcgccc tcctcgcggt cgcgctcgct gccggcgccg gcgcctacag
      481 ggcctacgcc cagaagaagc gcgccgcaga gacgatcggg cggcagaacg cggagctcct
      541 ggcaatcgac cggatcgcgc gggacatcaa caaggaggtc gagctccggt ccctgatcga
      601 gtcgatactg cggcactcgc tcgagcactt tccccaggcg gcgcggggcg gattcctcgc
      661 cctcgccgac gaggactccc gctttcgttc cctcgtctcg ttcggctacc ccgaggcggc
      721 gctcgagaag gtcgcgctcg cggaggaggc ggcccgggcg aggtacgtcg aggcggggga
      781 ggaggtcgcc gaggggctcc acgtcgtgag gcggccgggg tcgctggagg gcctcggaga
      841 ggcgtaccgg ccggcggcgt cgctcctcgt gttcgtcatc cgtgacgaga gcgcgacggt
      901 cggctacctc gtcctggaga gcgtcgacga tcccgccgcg ttcgaccgga tcgacgtgcg
      961 gcaggtgaag accctgcacg agcacatcat ctcggcggtc cagaaggccc ggaggctccg
     1021 gcaggtggag cggctctcgc ggaccgaccc cctgacccgc ctcgcgaacc ggcgtcacgt
     1081 ccaggagcgc ctggctctcg aggcggagcg cgccagccgc tacggaacgc cgttcctcgt
     1141 ggcgatgtgc gacctcgacg acttcaagaa cgtgaacgac acgctcgggc acgactgcgg
     1201 ggacgaggtc ctccgctcga cggcccggct cctctccgag aacctccggc gcaccgacct
     1261 cgtcggccgg tggggcggcg aggagttcct cgtcctcctt ccggagacgg agcgggaggg
     1321 ggggcggatc gcgctcgaga aggtgcgcgc gaagctcgag gcgctgccgc tcgtctacgc
     1381 cggagcgccg ctccgcgtga cggtctcgat cggtctcgcc gcgttcgtcc gaggggagcg
     1441 gctggaggac ctcgtcgccc gtgccgacgc ggcgctctac gaggccaaac gctccggaaa
     1501 gaaccgcgtc gtcgtcgcgg ggggctgacg gggaggtcca gcggaggcac accttcgcgg
     1561 cgcgccgaag accgacgtcg gccgccgccg atcggtccgg ctcccatcag gcggtcggag
     1621 cgggccccgg gaacgtctcg tccctcgccc ggagggaagg gctctccccg gagatcgaag
     1681 gcaggccgcg ccccgccccc tcgaacgctt ctcggagcga gcgggccgtc gccggtcgta
     1741 cgcgctcgtc ccgcgcgagc gcggcgagga ggaccgcggc gagggacccg ggacagtcgg
     1801 gccggtagcc ccgcgggtcg ggaacgggaa gtgtgaggac cctctctgcc gtccgggtgc
     1861 tgctgcttgc cgcgaacggg ttcactccgg tcagcgcctc gtagatcgtg agcgacagcc
     1921 cccagagatc gaagccgggc gacggaggat gcccctggag cgcctccggt gagagatagg
     1981 ggaccgttcc gacgacgtgg ctcgccgccg tgcgagtcga ggagtcggtc tccccacggg
     2041 cgcctcgcag gtcgggcggg acaccgggcg cgaaggacgc gtccgtctca ccgaggaggc
     2101 ggacgaggcc gaagtcgagc agctttggcg cgccgtccgc cgcgaatccg acattcgagg
     2161 gcttcaggtc acggtggagg acgccgcggg cgtggagggc ctcgagcccc gatgcgagcg
     2221 ccgcccccca ggccgcggcg agcgccgggg cgaccggtcc gcttcggatc ctgtcggcga
     2281 gcgttccgcc cggcaggtac tcgaggacga gcatgggcgt gccgtgccag ctctcgagcg
     2341 cgaagatgag gccgaggctc gggtggatga gctgggctcc ggcgcgcgcc tcgcgttgca
     2401 gccgcctcac ggagccggcg gagaggctcg ggagcgtctt gacggcgaca tcccggccga
     2461 gcgccaggtc gcgggccttg tagatcacgc ccataccgcc tgcgccgacc cagcgctcga
     2521 gccggtactt cccggcgagg agatacgggg ccggcacggt ctcgaagaac gtctcgtcgt
     2581 cggggcaggc gctccccgcg tcggagggaa agaggcgacg gcagcggcgg cagaggcgcg
     2641 ccgcttcggg ggcctcgatc gcgtcggtag gccggccggc gtgggagggg cccgccgaag
     2701 aggcctggga ggatcggagg aggaggttct cgagggcgag cgcgcccgca gaggcggctg
     2761 cggagaggaa ctgctcgtct tcggggtcga acgggcgctc gctcgccttt tcgccgacgg
     2821 cgagcagggc gagaagcccg cccgtcgagc ccgtcagcgg gacgaggatg cgggcgcggg
     2881 aggcggcgag ccagttccgg tccatctccg agaggtcggc gaagccggcg gcgtcgccgt
     2941 cgaggcggat cggcccgggc cgcagggccg cggcctcgac gatcggcgag gtcccgtcga
     3001 gcgcggggag cggccggccc gggctcacga aggatccggt ccgatcgtcg cgaacgaaaa
     3061 gggtcgacgg ctcggcgacg agcgccctct cgatcccgcc ggagaaggcc tcggccagct
     3121 cctcgacgtc gcgggctcgg cgcgcctcgc cggccaggct cgccagagtc cccgacgacg
     3181 ccttccgggc gtcgaagaag agccgctcga ggaggctccg cgtccggtcg ccgtatcgca
     3241 gccccgccgt cgtcgcggcg agcgcggcgc agatgaagag ggcgtgcgac gagaagacct
     3301 ctccgatcgg gacctcgcgc cgagtccaga agagcaggcc gagagcggcc aggggggcga
     3361 gcgagacgac ccgcacgctt ccctcggcga aggcgaggag gatcgccttc ttgaccagga
     3421 gcccgaccga gaggacccgc ggggagagca tcgcgtaggc gaggacgagc gggagaatca
     3481 ggaccgggag gagtgagagc gcgatcgcga cgggaggaag gagcagccgg ccggtgccgg
     3541 cgatccaggc gatctgcgcg agcgcggcgg cgaggggcgg cccgagcccg acgaggaggc
     3601 cggcgccgat gacggaggcc ctgcgccggg tggctgagga gagcggctcc atccggaggg
     3661 cgcgcgcctg gaagaagaag aggaggagcc cgccgagaga accggccccg aagaggaacg
     3721 acgtgagccg ccgggtccct ccgaggagga acgcctgcgt gcccgtgccg agcgaatcga
     3781 gcgtgccggg gacctggaag agcgggacga gaagcgcctg ggcgagcgcc gtcgcgccga
     3841 ggaggccgag gaggactcgg cacgcgcgcg accgcgtcag gtccgacggg aagagggaga
     3901 ggaacgccgc gagggctggg aaggcgaagt gcctcgggaa ggagacccac acgaggacga
     3961 tggcctcccg cagcggggtg ctctgaacga gtgaggcgtt cgcctcgagg aagcgtgctc
     4021 cgacgaagtg cccgagtccg gcgagcgccg ccccagccag gagcgcctgg aggccgacgc
     4081 cggcgagcgc gatcccagct ccggccagcg cgagcgcgac gccgaggagg gcgaatgtcg
     4141 cccgagtcac cgactcgtcg acggtcgacc gccgctcgcg gaccgcaccc gtgacgaccc
     4201 gggcgccggc gccggcttcg cggaaggcga cgggagtgcc ggccggcagg tcgaagaggc
     4261 tgcgcgccgc gacgacgccg ccggcgggcg cccatggtcc gccgccgatc gagatcgcgg
     4321 agaaccgcgt cgtccggccg tccgcgagga ccgtcccgcc gagggaaccg tccggcgccg
     4381 ggatcgactc gagccggacg gggcgctccc gcgtcgtccc gatgcggagc aatgcgccgg
     4441 gcaggccgag gaggacgagc agggcgcccc acagcgcgta ggcccgggcg agcctcgaaa
     4501 ggatcgtctt gaccccgatc atcctgcccg cggattatcg gggcaaacgg aatcgaagct
     4561 catcgaggag gtcgacatgc cgttcgacag gcgctggctc ctgaccgccg ccggctctgc
     4621 gttcctcgcg tcgctctcga aggacgggct cgcgacgccg cccactgcgg gcgcgaagcc
     4681 gacgggagac cccgcgaagg acaaggagct cgcattccag gaattccgga tcgggtacgg
     4741 cgacctgacc gccgacgagg agaaggtcgt ccggaaagcg ctcgagggga cgtacggaga
     4801 gcgattcgat cgtctctacc cgagcctcac cgaggccgag aagagaacgc tcctcggatt
     4861 cgcctgcttc ctcgggaccc tcacctcgag atgcctcgag aacgagcaga agggacggtg
     4921 cggctggaag ggggacccgt cggcgacgct gcgcgaggag cacgtgcagc gtgcccggga
     4981 gctcctcggc taccgcctca acacgaagct gaagttcccg tgctcctcgg cggtctcgtc
     5041 accgaatgtc cttcaggccg acgagccctg ccccctctgc ccgggctgag ggacgcggcg
     5101 agcctgcgct tcggaacccg caccgcgtcc cgccccgcgc gagacgcgcc ttcgagccgg
     5161 cgaagtctct ccagaccagg tcggtcgctt tcgcgtggca ggggaggcag gcgctgacgg
     5221 cgagggcgcg gcggagctcg gccgcgtcga gcgagcggag gccgacgcgc gtgccgatcc
     5281 cgggcgaggg ggcgtcgagg agggtccagc cgtcgacggc gaggcgcgcg tcgtccgtcg
     5341 cgggagaggc gggagagaac gacgccgcgc cgctctcgaa cgagagggtc ccggtgccga
     5401 ggccgagcgc ccacgacgag aggtggcagc tcgcgcagtc gcgcgccttc ttcccggtgg
     5461 agtgcgggtc gagggacgca tagagccggc gagagaagcg cggtccggag gccgcggcgg
     5521 cgtcgaggtc catcaccatc ccggggatcg ccggggcgat ccggccgtcg ggtctcacgg
     5581 cgagggccgg aggccgcgcg tcgtacccct ccgacgcctc gacccaccgg ccgggcgtct
     5641 cggcggcctt cgcgaagtcc cactgcgcgc cctcggcgtc gtagcgcgtg tggcaggtcg
     5701 agcaggtcgg cgcccacgcg gagtggcagg cgctgcacgt gaggcgctcg tggccgggga
     5761 gacggtggtt cgcgtcggcc ggagtcggcc tcgtggcgag cgcgactccc tgccgcttgc
     5821 ggagcgtcga ccacccgccc tccgacgggc ggaggttcca gaccggcgtc ccgtgccggc
     5881 cgaggcggac acgctcgccg ggcggccgcg tctccccctg ctgccggagg agatcgcggg
     5941 tgatcgggtc cctcacctcg gcccaggtcg tctcgcctcc gtcgcgggcg gggccgtggc
     6001 acgcctcgca ggcgatctcg acctggctct cggagtgctc gtgcctggtc ccgtcgccca
     6061 tcaggtcggt gtggaggtgg cagtcgacgc acgagaggcc ggcgcggcgg tggacgtccg
     6121 cctcggcgtg cccggcgggg cgcccgtcgt ggagcgtcgt cgccgccgtg ccgagcgcgg
     6181 cggcctgcgc cggctcgatc tcgtagagcc cctcgtaggt cagcgcgatc cggccgctcc
     6241 ggctgtggca gccgagacag cggtcgtcgg tgacgtgcgc gtcgacggag gggtgcggcc
     6301 gcgggaccgc gcccgggagg cggcgcgcga cgtggcaggc cgagcagccg ctgccgttgc
     6361 cgtggatcgc gtcgtcccgg ctggcgcgga gggcgtggag gtggcagccc gcgcagagct
     6421 tcgcgaggtg atcttccgcg ggggtccgct cctccttcgc gaggagatcg accatcgtgg
     6481 ccgtgccgcg cggctcgggg acctcgccga aggcccaccg gttcaccgag acgatcccgc
     6541 tcgcgcgcgc catgagcgag gtggcgacgc gggccgcctc gcggacgtga cagccgctcc
     6601 ggccgcaggt ccgcgcgacg ctccggagcg cgcccggctc gggctccatc ccggcgtgcg
     6661 cgcgcgcctt gtcgaacgcg aacgggttcc cgaggtggca ggaggagcag ccgacggcgg
     6721 cgcgggcgtg cgccccgccg gggtcctcct cggggcggac gtggcacgtc acgcagcgct
     6781 ccaccttccc gcccgcgcga tcgggagcgc gcccgagagc cgcggcgcgc tgccgctcgc
     6841 gcccgagggc gccgagcagc cccgcgagcg cgaggaggag cgcgaggagg agcgcctggg
     6901 cccggaacat cagcgggcgg cgaggaggag gcggagcgtc agggcgaggt agcccgcgcc
     6961 ccaggcggcc gcgagcgcga ggagggccgc acgggcgcgc ccgtccgcgt gccggacgag
     7021 cccgaccgcg aggaggaggg cgagcgggag gagccagccg gccgcgacgg gaaggtcgag
     7081 gagggctccc tggaggccga ggagatacca ggggccgagg aggagagcgc cgtccggcgc
     7141 gaccggcggc ccgagggaga cggggaagag gcccgcggcc gcgaagctcg cgagcgcggc
     7201 gaggacggtc gatcggaggt cggggaggag cgtcccgccg tgctcggccg tcaggagcca
     7261 ggcgaggagc gtgaaagtcc cggcgtggtg gagcgagacc ggggcgaggt cggcgccggg
     7321 gaggccgagg aggagcgcgg cgagcgcgcg gccggcgatc gggacgctcg acgagacctc
     7381 gcgccagacg gcgagcgcgg cctgcgcctc ggcgtccccc ctcatcagga agccgccgag
     7441 gagcgcgagg acggccacag gcaggagggc gaccgagcgc caccagacgg ccggccgaag
     7501 acggctctcg gtccgcttcc agaggacctc cccgaggtgg gcgagcgtcc cgaggaggaa
     7561 cccccacgcg gagaacgcgt ggacgccgcg gaggaagaag ccccacgggg tcgccccgtt
     7621 gagcgtctcg aggctctcga gcgcacgccc cgccgaccag aacggggcga gggcgaggcc
     7681 ggagaaaagc gcgacggcga ggaggccgaa cgtgacgcgg ccgaggatca cgagaggcgc
     7741 gccacgaatc ccgcgccgtc gggggagagg cggggccggg ccagcggccg cgaggcgggg
     7801 cccgccgtca cgcgcccttc ggcgtcgtaa cggctcgcgt ggcagggaca cgagaatccc
     7861 ccgccctcga ccggagcgac gcggcagcgc aggtgcgtgc aggtgaggtc gaggacgacc
     7921 ggggaggcgg ccgggccgcg gacgtggagc ccttcgtgga cgagccctcc ctccttctcg
     7981 gcgcgcgcga ggagctcggc ggggaggcgg acgcccctct cgggctcggg agacccggag
     8041 agggcccggc cgaggacggc cgtgccggcg gccgccgcgg ccacgccggc ggcggcccag
     8101 gcggttccga ggaagtcccg gcgggagctc actcgatccc gagctccttc atccgccgcc
     8161 agagcgtgct ccggctcatg ccgagcagct cggccgcacg ccctcggttc caccgcgccg
     8221 actcgagcgt ctggaggatc cgcagcctct cggccgggtc ttccgggagc gcatcgccga
     8281 gcggcgcggc gtcctgcgcc gaggccggct cgaggacgct ccgctccgct ccctgccgca
     8341 gctcgggcgg caggttctcg atctggatcg tctggcccgt gcaggtcgcg acggcgtact
     8401 cgagggcgtt gccgagctcg cggacgttcc cgggccacgg gtagcgggcc atcgcggcga
     8461 gcgtgtcggg agagaggagg agcgcccggc cctcgcgccc tccgatgtgg gcgaggaggt
     8521 ggtgggcgat gaggggaatg tcctcgaccc gctcccggag cggagggacg tggatcggga
     8581 cgacgcgcag gcggtagtag aggtcgtcgc ggaagcgccc gacccggatc gcctcggcga
     8641 ggtcgacgtt cgtggcggcg atgacccggg cgtccatcgg gcggggcgtg ctctcgccga
     8701 cccgctcgaa ccgcctctcc tggaggacgc ggaggagctt gacctgcagg tgcggcggga
     8761 tgtccccgac ctcgtcgagg aagagcgtcc cgccccgggc gaggtcgatg cgccccacgc
     8821 ggtcgcggac ggccccggtg aaggcgcccc ggacgtggcc gaagagctcg ctctcgagga
     8881 ggtcgcccgg aagggccccg cagttgacgg cgacgaacgg cccggtgcag cggggggagt
     8941 tcgcgtggag cgcccgggcg atcagctcct tgccggtgcc gctctccccg gtgacgagga
     9001 ccgtcgcctc gctccggtgg agcgactcga cgaggtggac gatcccgcgc atcgccgggg
     9061 agcgggccac gaggccgtgg gacgagagca tcgactgcac gaggccgttg tcggtctccg
     9121 ccggacggag gacgacgatg tagcgggccc gcgggtcgca gtggctcgtc acgtcgctcg
     9181 ggaggaccgc ggcggtgagc gagacgaggc gggcgctctc ctctccgcag cggaggatgg
     9241 cccggcggcc ctcctcgcgg tgcccctgct cgagcgcttc ccggagcgac tccgacggcc
     9301 cgaagaggcg cgctccgacg agctcctcga tcggacggcc gagggccgcc tcgacggccc
     9361 cgggacgggc gaggtcgtcg agcgagcggc tcgcgcggat gacacggaac tcgcggtcga
     9421 ggacgaccag gacccggccg agcgcggcga agacggtctc gagggtgccg aagccggggg
     9481 gagccaggga ggacgtctgg ggggcggaca gggtgtcggg cacaggcgta atctcttcgt
     9541 tccgggaccg gcccgattat tccatcccga gggtgcgcat gacgtcgatc tacttcggcg
     9601 aggagcacga gagctttcgc cgcgcggtcc gccagttcct cgagggagag gtcgcgccgc
     9661 gcgcgcgcga atgggaagag gcgcggcgga tcccgcgcga cgtcttccgc cggatggggg
     9721 acctcggctt cctgggcgtc ctcgcgccgg aggaggtcgg cggggccgac gcctcgatct
     9781 ttcacgcgat cgcgctcctc gaggagctgc cgcgctcgag gatgggaggg ttcgtcgcgg
     9841 ccgtctcggt gcagcagttc atcgcgacgg gggcgatcgc cctccacggc accggggagc
     9901 agaagcgccg ctggctcgcc ccgtccgtgg ccgggacgaa ggtgggcgcg atcgcgatct
     9961 ccgagcccga caccggctcc gacgtcgccg cgatccggac gacggcgcgg cgcgacggcg
    10021 acgcctgggt gatcgacggc gcgaagacct ggatcacgaa cggcgtcgag ggggacttcg
    10081 tcgtcgtcgc ctgccggacc gccgcggacg ccggcgccgg cgggatcagc ctcgtcgtcg
    10141 tcgaggccga cacgcccggc ttctcgcgct cgcgcctccg gaagatgggc tggcactgct
    10201 cggatacggc ggagctgacg ttcgcggacg tccgcgtccc gctcggcaac ctcgtcggcg
    10261 ccgagaacga gggcttcttc ctgatcatgg agacgttcgt cctcgagcgg ctcgtcacgg
    10321 cggcgacggc cgtcggcagc gcccgcctcg cgctcgagga gacccggcgc tacgtcctct
    10381 cgcgggaggc tttcggccgg cccatcggga agttccaggc gatccgccac cgcctcgccg
    10441 acctccacgc cgagctcgag gcggcctccc agctcgtcta ccacgccgcc tggctccacg
    10501 agaggggcga gaaccccgtc gccgaggcct gcatggc
//
LOCUS       DAOTEZ010000106        10455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4486_length_10455_cov_6.198462, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000106 DAOTEZ010000000
VERSION     DAOTEZ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10455
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4486_length_10455_cov_6.198462"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="P5164_07390"
     CDS             complement(<1..754)
                     /locus_tag="P5164_07390"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY43756.1"
                     /translation="MTGIRTGRDARTTLRNRLRRVMAVGGLVLLATTATGCQKVVDQF
                     KAKQIIRKGNAYFKEQLYEDALKQYEEAMKLDPSEIRIKKFVAMANMAVYNPGSQHPK
                     DLQALETAIKHFKEYLAVKPDDESAAKFLVTTYMNSRRYDDAIEYFKALIKAHPEDKQ
                     AVQTIAMLYARKGDFDQSMEWQQNLAKLDSQNPEVFYTIGVTCWDKSYNTPMDQMDGE
                     ARKAMIDKGMKALETANSLREDYFESMLYVNLL"
     gene            1275..3011
                     /locus_tag="P5164_07395"
     CDS             1275..3011
                     /locus_tag="P5164_07395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRY43757.1"
                     /translation="MVLSRPLASLAVATLAAVLVPQAVSAGPQQPTVSLPPLVRNVDV
                     TVTNIDVVVTDKKGSRIRGLKREDFEVYEDGQLQPLTNFFAVDGGKVVYFGDEAVPGA
                     PAATPVPSPAPEAAPAPAEPEAVPLPVPKTKIVIFIDNLSLQPFNRNRVLKNLETWAR
                     ENIRDNVEAMVVTWDRSLKVRRKFTNDGREISDVIRQQGDFSTMGQTRLSERRDILRR
                     IDDAQSEYEAFVEARQYALAYKNDLDFTTDALKRTIDQLAGVEGRKILLHVSEGLPQS
                     PGAELWKYIQDRFRTQSGGLTQFEFDSTSAYLGIIRAANAAGVSMYMVDASGLSIDEG
                     ISAENRSTQAKIDTFVEKTNLQSMIHLMAEETGGKAILNRNDITGPLAEMKSDYTSYY
                     SVGYRSLRSGGERPRKVEVKVKQKGLVARSRRSYVEKGLETRIVEGVTSALYFARDEN
                     PLGVGIEVGQPAPADAQNYLVPIRIRVPYSRITVLPQGDVARGRIALYFIVIDAAGKQ
                     SELATQVVPVEIASKELDALNRRDFVYDVRLLMIPGGQRLSLAVRDEPTNTVSYVQQA
                     IFVSALPQETPR"
     gene            3040..4545
                     /locus_tag="P5164_07400"
     CDS             3040..4545
                     /locus_tag="P5164_07400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRY43758.1"
                     /translation="MIALGPAAAREADARTIAAGTPGIVLMERAAEAVARECLRAIAG
                     VPLRGERVVVLCGTGNNGGDGFETARLLRRSPRVGAVAVLLLGARARLAGDAAETLRR
                     LERDGGEVAEVTAPEGLEPLRGATLVVDALFGTGLSRPLDEGALAAHATRIASGRRAF
                     VVSVDLPSGLDAASGVPVGPHVRADLTVTFGAPKLAHLLLPAAAACGRVVVADIGLLP
                     AGPTADGPEAVAALDVAPLFPPRPAAAHKGTYGTLAVVGGALGMAGAAALAARAAFRA
                     GAGKVVVVAEGASRPIVHALVPEATSVEALPGSGPTALAVGPGLGTGSWGAGRLDEAL
                     ATALPVVLDADALNLLRGKLGTLVGRPGPIVVTPHPGEAARLLGRTASSVVGDPESAA
                     RRLAAESGATVVLKGFRSVVATPAGRLARVLSGNPGLATGGSGDVLTGIVGALLARGR
                     TAWEAASAGTYLHGLAGDLVRERLGEESLRASDLPEELGRAFAAVAESLLP"
     gene            4542..5006
                     /gene="tsaE"
                     /locus_tag="P5164_07405"
     CDS             4542..5006
                     /gene="tsaE"
                     /locus_tag="P5164_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRY43759.1"
                     /translation="MKSVPFEAVARSVEETREVGRELSLLLVPGTLVRLSGPLGAGKT
                     ELVRGLAEGLGIHPDEVASPTFALVHEHGPEGRPALLVHADLYRLLDDERLEPAIEGL
                     GLDEAREEGAVVVVEWPEGLPAGRGDFEVKVTPEENGARRIEVRRAPGESGA"
     gene            complement(5013..5678)
                     /locus_tag="P5164_07410"
     CDS             complement(5013..5678)
                     /locus_tag="P5164_07410"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRY43760.1"
                     /translation="MKRFPRIALLAVAAAVLTAGPAAAQDRRGTVEITPVVGGYFGGT
                     FDPGTLAFYDGRADASTEVAYGLKLGFNITNNFAIEASYLQSDPKLRLEGGGAIGGGS
                     EDIGKMEMRLYELNFLVPWGRGPVRPYFTIGGGVNTFRPSIPGYSFSSDSRFTGSLGL
                     GVKTYVNPNFGFRFEGKVRSTYINSEDDHWSCDSWWCDGDYYGDSQWYTAGEATAGVV
                     IAF"
     gene            5849..6286
                     /locus_tag="P5164_07415"
     CDS             5849..6286
                     /locus_tag="P5164_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRY43761.1"
                     /translation="MTRLERLLGCFAAGVLLPGAAGCLGTGTHRVDAPCREMRASVAR
                     EMLRDSREIPLLDVRALPAPRLRGAHEIPLFALRSRIEELGRFRGSPLVVVADSGEAA
                     RQACELLAAEGFRYVIFVPEGAEGLFAGVRGAETAADAPKERR"
     gene            6283..6747
                     /locus_tag="P5164_07420"
     CDS             6283..6747
                     /locus_tag="P5164_07420"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRY43762.1"
                     /translation="MKRVVVGKGAQGALLLAYTALVLAGTLLVEGRPGVVTNLVPFDD
                     LARLRASAGKAGVLSNRFVLGLLGLVGNLVMFAIWGFLAWKFVDGRGRARWRNHAEVV
                     FFGLVFSVGIETVQFFLPTRAADVNDVFWNAVGALLGALLAHVHASVRVEWA"
     gene            6776..6861
                     /locus_tag="P5164_07425"
     tRNA            6776..6861
                     /locus_tag="P5164_07425"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6809..6811,aa:Leu,seq:caa)
     gene            7010..7531
                     /locus_tag="P5164_07430"
     CDS             7010..7531
                     /locus_tag="P5164_07430"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HRY43763.1"
                     /translation="MGFNIGDKLVYPNHGVGVVETIGESLFDGRAHPCYQVRLLANNS
                     KVVVPVGNSDRVGLRPLTRRQDVTGVFKVLEDGSFQSNGDWKGRFKQNLDKMRSGRLS
                     DIADVLKNLNWVQKQKTLSFREKKMYERARYLIVSEIAQVNGLPEAEVEQDVEKALDR
                     SVARRRSLGPAAR"
     gene            7581..8333
                     /locus_tag="P5164_07435"
     CDS             7581..8333
                     /locus_tag="P5164_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43764.1"
                     /translation="MSHRAPAGLLLVLALGVPLVAAARTDPSMESLLVALDLERRARN
                     EDVLELERLTSAATRADAAAASARERLVDSVRGGDVQAGDLRDEEERVVEAEARAAAA
                     REIRRAAVARLVERVRRIALLQEEVARRRSASRRAADPVTGRWEATIDPGARRGAFRL
                     VLDGTLVSGDYTLDGGFRGSLRGTYVGERITIQRIDSERGFDATFYGRVQPQLKRITG
                     TWEATAIAPATGPVAGTWSAALLPDDEDGGLP"
     gene            8414..9046
                     /locus_tag="P5164_07440"
     CDS             8414..9046
                     /locus_tag="P5164_07440"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY43765.1"
                     /translation="MAPSAPRWGDRLLFAALGALVGFAGAWVALDGRHAHVPQPPAMA
                     MDDPHAGVPGAPPLGANAGAPAAGGASFDRAAWQRLQDAQAAATAKPDDYDALVQLGN
                     AAYDAHEYAQAIEAYERALKVRPGDPNVMTDLGTAYRNEGKLDEALSLFRKARAANPS
                     HWQSLYNEVVVLALDKHEHDAADAALARLKKEHPGLPAIENLERQIGAAH"
     gene            9088..10407
                     /locus_tag="P5164_07445"
     CDS             9088..10407
                     /locus_tag="P5164_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRY43766.1"
                     /translation="MPDRRATPSVAPLAERFRPRDLSEMRLPASVAGPSSLLGRAIEA
                     DRVPSLVLWGPPGTGKTTLARVIATRTKARFLSFSAVVSGVKEIREVLEESQRLATRG
                     ERTLLFVDEIHRFNRAQQDVFLPYVESGAATLVGATTENPSFELNGALLSRMRVVVLP
                     PLSREDLESILGSAAADAERGLGGRVTLGEGAVDWLVGFSDGDARRALNALETAADHA
                     GEGAVLTAALLGEIYARKSLLFDKSGEEHYNLISALHKSVRDSDVDASLYWLARMLEA
                     GEDPLFVARRLVRASTEDVGLADPNALRLAMAARDAVHFLGMPEGALALAEATVYLAL
                     APKSNALYAAYGEAARDVAETPNAPPPAAILNAPTRLMKDVGYGKGYVYAHDTAEGTA
                     GLSCLPDALAGRRYYRPKGTGREAEAKRRLDELRELRERLRRERHRR"
BASE COUNT         1452 a         3821 c         3655 g         1527 t
ORIGIN      
        1 agaggaggtt cacgtagagc atcgactcga agtagtcctc gcgcagtgag ttcgccgtct
       61 cgagcgcctt catccccttg tcgatcatcg ccttgcgggc ctcgccgtcc atctggtcca
      121 tcggcgtgtt gtaggacttg tcccagcagg tgacgccgat cgtgtagaag acctcggggt
      181 tctgcgaatc gagcttcgcg aggttctgct gccactccat cgactggtcg aagtcgccct
      241 tccgcgcgta gagcatggcg atcgtctgga ccgcctgctt gtcctcgggg tgggccttga
      301 tgagcgcctt gaagtactcg atggcgtcgt cgtagcgcct cgagttcatg taggtcgtca
      361 ccaggaactt cgcggcgctc tcgtcgtccg gcttgacggc gaggtactcc ttgaagtgct
      421 tgatcgccgt ctcgagggcc tggaggtcct tcgggtgctg cgaccccggg ttgtagacgg
      481 ccatgttggc catcgcgacg aacttcttga tccggatctc gctcggatcg agcttcatcg
      541 cctcttcgta ctgcttcagg gcgtcctcgt agagctgctc cttgaagtag gcgttcccct
      601 tccggatgat ctgcttcgcc ttgaactggt cgacgacttt ctggcatccc gtcgccgtcg
      661 tcgccagcag gacgagcccg ccgaccgcca tcacgcggcg aaggcggttc cggagggtcg
      721 tccgggcgtc tctccccgtc ctgattccgg tcatcggctc tctctccttt cgcgcaccca
      781 cggtgcccgc ggtcctgaga cccgggagac caccgttccg ttcccggtca gacatatgct
      841 tcgctgctcc ccgtcgcccg cttccatcgc tcgatgggtc gcgaccgtcc cgaaaaggtg
      901 tcgagatggg aggcgagccg ggacgtcacc gcgggctcct cccgagggag gtcggaaccc
      961 gacgggcgca ttcgacgtaa aggagaaagg cccggaggta gtccgcgtcc gcgcgcaccc
     1021 cgccgttgtc tgcctccgcg tccgttccca gctgcggctg tctcagcccc gacctccgga
     1081 tcttccctcg acctctcgga tcgtccgcgc gcccaccggg ctcggggcgg ccgtcattcg
     1141 aggcggacat tctcacggag cacggggcga agtgcaagcg gaaaactcct cggaaacagg
     1201 ccccgatcgg ggactgtagc ggtcctctcg accgcatcgg aacgcgtcgg cggcgcgatc
     1261 cgtagaatgc gtccatggtg ctttccaggc cgctcgcgtc cctcgccgtc gccaccctcg
     1321 ccgcggtcct cgtcccgcag gccgtttcgg ccggaccgca gcagccgacg gtcagcctgc
     1381 ctccgctcgt ccggaacgtc gacgtgaccg tgacgaacat cgacgtcgtc gtcacggaca
     1441 agaaggggag ccggatccgc gggctcaagc gcgaggactt cgaggtctac gaggacggcc
     1501 agcttcagcc cctgacgaac ttcttcgcgg tcgacggcgg caaggtcgtc tacttcggcg
     1561 acgaggcggt cccgggtgct ccggcggcga cccccgtccc gagcccggcc cccgaggcgg
     1621 ccccggctcc ggcggaaccg gaggccgtgc cgctgccggt gccgaagacg aagatcgtca
     1681 tcttcatcga caatctgagc ctccagccct tcaaccggaa ccgggtcctg aagaacctcg
     1741 agacctgggc tcgggagaac atccgggaca acgtcgaggc gatggtcgtc acctgggacc
     1801 gctcgctgaa ggtccggcgg aagttcacga acgacggccg ggagatctcc gacgtgatcc
     1861 ggcagcaggg cgatttctcc acgatggggc agacccgcct ctccgagcgc cgggacatcc
     1921 tgcgccggat cgacgacgcc cagagcgagt acgaggcctt cgtcgaagcg cggcagtacg
     1981 cgctcgccta caagaacgac ctcgacttca cgaccgacgc cctcaagagg acgatcgacc
     2041 agctggccgg agtcgagggg cggaagatcc tcctgcacgt ctccgaaggc ctcccgcagt
     2101 ccccgggagc cgagctctgg aagtacatcc aggaccgctt ccggacgcag agcggaggcc
     2161 tgacgcagtt cgagttcgac tccacctcgg cctacctcgg gatcatccgc gcggcgaacg
     2221 cggcgggcgt gagcatgtac atggtcgacg cctccgggct ctccatcgac gaggggatca
     2281 gcgcggagaa ccgctcgacc caggccaaga tcgacacgtt cgtcgagaag acgaacctcc
     2341 agtcgatgat tcacctgatg gcggaagaga ccggcgggaa ggcgatcctg aacaggaacg
     2401 acatcacggg gcccctcgcg gagatgaaga gcgactacac ctcgtactac tcggtcggct
     2461 accggagcct tcgctccggc ggcgagcgcc cgcgcaaggt cgaggtgaag gtgaagcaga
     2521 aggggctcgt cgcgcgctcg cggcggagct acgtcgagaa ggggctcgag acgcggatcg
     2581 tcgagggcgt cacctcggcg ctctacttcg cccgcgacga gaacccgctc ggcgtcggga
     2641 tcgaggtggg gcagccggcg cccgccgacg cgcagaacta cctcgtcccg atccggatcc
     2701 gcgtccccta cagccgcatc accgtgctgc cgcagggaga cgtcgcgcgg ggccggatcg
     2761 cgctctactt catcgtgatc gacgccgcgg gcaagcagtc cgagctcgcg acgcaggtgg
     2821 tgccggtcga gatcgcctcg aaggagctcg acgcgctcaa ccggcgcgac ttcgtctacg
     2881 acgtccggct cctgatgatc ccgggcggcc agcgcctctc gctcgccgtc cgcgacgagc
     2941 cgacgaacac cgtcagctac gtccagcagg cgatcttcgt ctcggccctg ccccaggaga
     3001 cgccgcggtg atcgccgccg cgcgacggcc ggagggggcg tgatcgccct cgggccggcc
     3061 gccgcgcgcg aggcggacgc ccggacgatc gcagcgggaa cgcccgggat cgtcctcatg
     3121 gagcgggcgg cagaggccgt cgcccgcgag tgcctgcggg ccatcgccgg agtccccctg
     3181 cggggcgagc gggtcgtcgt cctctgcggg acggggaaca acggggggga cggcttcgag
     3241 acggcgaggc tcctccggag gagtccgcgc gtcggtgcgg tcgccgtcct cctcctcggt
     3301 gcgcgggccc gcctcgcggg ggacgcggcc gagacgctcc gacgactcga gcgggacggt
     3361 ggcgaggtcg cggaggtgac cgcgccggaa gggctcgagc cgctccgcgg cgcgaccctc
     3421 gtcgtcgacg ccctcttcgg aacggggctc tcgcggcccc tcgacgaagg agccctcgcc
     3481 gcgcacgcga cccggatcgc ctcgggtcgc cgcgcgttcg tcgtctccgt cgacctcccg
     3541 tcggggctcg acgccgcctc gggagtcccc gtcggtccgc acgtgcgtgc cgacctcacc
     3601 gtcacgttcg gcgccccgaa gctcgcccac ctcctcctgc cggcggcagc ggcctgcggt
     3661 cgcgtcgtcg tcgccgacat cgggctcctt ccggccggcc cgaccgccga cggtcccgag
     3721 gccgtcgcgg ccctcgacgt cgcgcctctc ttcccgcccc ggcccgcggc cgcgcacaag
     3781 gggacgtacg ggaccctcgc cgtcgtcggc ggcgccctcg ggatggcggg ggcggcggcc
     3841 ctggcggccc gggcggcgtt ccgggccggc gcgggaaagg tcgtcgtcgt cgccgaaggg
     3901 gcgagccgcc cgatcgtcca cgcgctcgtg cccgaggcga cctccgtgga ggctctgccg
     3961 gggtccggac cgaccgccct cgccgtcggc cccggactcg ggacgggttc ctggggagcc
     4021 ggtcgactgg acgaagcgct cgcgacggct ctcccggtcg tcctcgacgc cgacgccctc
     4081 aacctgctcc gagggaagct cgggacgctc gtcgggcgcc ccggcccgat cgtcgtgacg
     4141 cctcaccccg gggaggcggc ccgcctcctc ggccgcaccg catcctccgt cgtgggcgac
     4201 ccggagagcg ccgcccgtcg cctcgccgca gagtccggcg cgacggtcgt tctgaagggg
     4261 ttccggtccg tcgttgcgac cccggcgggg cgcctcgccc gggtcctgtc cggcaatccc
     4321 ggcctcgcga ccggagggag cggcgatgtc ctgaccggca tcgtcggagc gctcctcgcg
     4381 cgcggccgga cggcgtggga ggccgcctcg gcggggacct acctccacgg cctggcgggc
     4441 gacctcgtaa gggagcgcct cggcgaggag agcctccggg cttcggacct ccccgaggag
     4501 ctcggccgcg ccttcgcggc cgtcgccgag tcgctcctgc cgtgaagagc gtcccgttcg
     4561 aggcggtcgc tcgctccgtc gaggagaccc gcgaggtcgg ccgggagctc tccctcctcc
     4621 tcgtccccgg aactctcgtc cgcctgtcgg ggccgctcgg cgccggaaag accgagctcg
     4681 ttcggggcct cgccgaaggg ctcgggatcc acccggacga ggtcgcctcg ccgacgttcg
     4741 cgctcgtcca cgagcacggc ccggaagggc gtccggctct cctcgtccac gcggacctct
     4801 accggctcct cgacgacgaa cggctcgagc ccgcgatcga ggggcttggc ctcgacgagg
     4861 cgcgggagga aggggccgtc gtcgtcgtcg agtggccgga gggccttcct gccgggcggg
     4921 gagacttcga ggtgaaggtc acgccggagg agaacggggc ccggcggatc gaggtccgcc
     4981 gggccccggg cgagtccgga gcctgacggc gatcagaagg cgatgacgac ccccgccgtc
     5041 gcctccccgg cggtgtacca ctggctgtcg ccgtagtagt cgccgtcgca ccaccagctg
     5101 tcgcaggacc agtggtcgtc ctcgctgttg atgtaggtgg agcggacctt gccctcgaag
     5161 cggaagccga agttcgggtt cacgtaggtc ttcacgccga ggccgaggct gccggtgaac
     5221 cgggagtcgg acgagaacga gtagcccggg atcgaggggc ggaacgtgtt cacgccgccc
     5281 ccgatcgtga agtaaggccg caccgggcca cgcccccagg ggacgaggaa gttgagctcg
     5341 tagaggcgca tctccatctt gccgatgtcc tccgagccgc ctccgatcgc tccgcctccc
     5401 tcgagccgga gcttcgggtc cgactgcagg tagctcgcct cgatcgcgaa gttgttcgtg
     5461 atgttgaagc cgagcttcag gccgtacgcg acctcggtcg aggcgtccgc ccgaccgtcg
     5521 tagaaggcga gcgttccggg gtcgaacgtc ccgccgaaat agccgccgac gaccggcgtg
     5581 atctccaccg tgccgcgccg gtcctgcgcg gcggccgggc cggccgtcag gaccgccgcc
     5641 gcaacggcga ggagggcgat tcgcggaaat ctcttcatgt ctcgatcctc caggtgtccc
     5701 gcgtccacct tttcggcctc tgggccgcac gcggaacgtt tcattctgag ccttagcaac
     5761 gtacgtaccg ccgcgaaacg ggttcgcgga cgtgctctcc gtcacgtctc gtccggtttc
     5821 ggggtcgcga tccggtaagc tcgccggagt gacccgcctc gaacgtctgc tcggctgctt
     5881 cgcggccggg gtcctcctcc cgggagccgc cggatgcctc ggcacgggga cgcatcgcgt
     5941 cgacgcgccg tgccgcgaga tgcgcgcgtc cgtcgctcgc gagatgctgc gggactcgag
     6001 ggagatcccc ctgctcgacg tccgggcgct tcccgcgccc cgcctccggg gcgcccacga
     6061 gatcccgctc ttcgccctgc gctcccgcat cgaggagctc gggcgctttc gagggtcgcc
     6121 cctcgtcgtc gtcgccgact cgggagaagc cgcccggcag gcgtgcgagc tcctcgccgc
     6181 cgaggggttc cggtacgtga tcttcgtccc ggaaggagcc gaaggcctct tcgccggggt
     6241 gcgcggggcc gagacggcgg ccgacgctcc gaaggagcgc cggtgaagcg cgtcgtcgtc
     6301 gggaaggggg cgcagggggc gctcctcctc gcctataccg ccctcgtcct ggccgggacg
     6361 ctcctcgtgg aagggcggcc cggggtcgtc acgaacctcg tgccgttcga cgatctggcg
     6421 cgcctccggg cgagcgccgg caaggccggc gtcctctcga accgcttcgt tctcgggctc
     6481 ctcggcctcg tcggcaacct cgtcatgttc gcgatctggg gcttcctggc ctggaagttc
     6541 gtcgacgggc ggggacgggc gcgctggcgc aaccacgccg aggtcgtctt cttcggcctc
     6601 gtcttcagcg tcgggatcga gacggtccag ttctttctcc cgacgagggc ggcggacgtg
     6661 aacgacgtct tctggaacgc cgtcggagcg ctcctcgggg cgctcctggc gcacgtgcac
     6721 gcctcggtac gagtcgagtg ggcctgacgg cccgctgcta gacttcgcgc tccgcgccgg
     6781 agtggcggaa cggcagacgc aggggactca aaatcccccg agcgcaagct catgagggtt
     6841 cgagtccctc ctccggcacc agctgctccc cgggtgcgcg gcagccccgg gccgtccggg
     6901 ggcgtccacc cccgcgcggg ccgccgggcc gctccgcatc ggcttcccat tgacccccga
     6961 ccgggggccg cgtaagctag caagtccgat agcgcaggag gcggatccgg tgggtttcaa
     7021 catcggcgat aagctcgtct atccgaacca cggggtggga gtcgtcgaga ccatcgggga
     7081 gtccctgttc gacggccgcg cccacccctg ctaccaggtc cggctcctcg ccaacaactc
     7141 caaggtcgtc gtcccggtcg gcaactccga ccgcgtcggg ctgcggccgc tcacccgacg
     7201 gcaggacgtg accggcgtct tcaaggtcct cgaggacggc agcttccagt cgaacggcga
     7261 ctggaagggg cgcttcaagc agaacctcga caagatgcgc tccggccgcc tctcggacat
     7321 cgccgacgtc ctcaagaacc tgaactgggt ccagaagcag aagacgctct ccttccgcga
     7381 gaagaagatg tacgagcggg cccggtacct catcgtctcc gagatcgcgc aggtgaacgg
     7441 ccttcccgag gcggaggtcg agcaggacgt cgagaaggcc ctcgaccggt cggtcgcccg
     7501 gcgccgcagc ctcggccccg ccgcccgctg agccccgaat ccggcgacga gcgcggcgcc
     7561 gctcccgtat agtcctgtcg atgtcgcatc gagctccggc cggcctcctc ctggtcctgg
     7621 cgctgggcgt ccccctcgtg gccgcggcgc ggaccgaccc gtcgatggag agcctcctcg
     7681 tcgcgctcga cctcgagcgc agggcccgga acgaggacgt cctcgagctg gaacgcctca
     7741 cgtccgccgc gacccgggcg gacgcggccg ccgcctccgc ccgggagcgc ctcgtcgact
     7801 ccgtccgcgg cggggacgtc caggccggcg acctgaggga cgaggaggag agggtcgtgg
     7861 aggccgaggc ccgagccgcc gccgcccgcg agatccggcg cgccgccgtc gcccgcctcg
     7921 tcgagagggt ccgccggatc gcgcttctcc aagaagaggt ggcccggagg cgctcggcgt
     7981 cccgccgggc ggcggacccg gtcaccgggc gctgggaggc gacgatcgac cccggagcac
     8041 ggaggggcgc cttccgcctc gtcctcgacg gcaccctcgt ctccggcgac tacacgctgg
     8101 acggcggctt ccgcggctcg ctccggggaa cgtacgtcgg cgagaggatc acgatccagc
     8161 gaatcgactc ggagcgcggc ttcgacgcga cgttctacgg gcgcgtccag ccccagctga
     8221 aacggatcac cggcacttgg gaggcgacgg cgatcgcgcc ggccaccggc ccggtcgcgg
     8281 gcacctggtc ggcggcgctc cttcccgacg acgaagatgg aggtttgccc tgagccacga
     8341 cgaccacctt ccccccccgc ccgaggcccg gaccggctcc cggggcgggg ctcgatcgaa
     8401 gcaggccgcc gcagtggccc cgtcggcgcc ccgctggggc gaccggctcc tctttgccgc
     8461 cctgggagcc ctcgtcgggt tcgccggggc atgggtcgcc ctcgacggac ggcatgcgca
     8521 cgtgccgcag cccccggcga tggcgatgga cgacccgcac gccggcgtgc ccggcgcccc
     8581 gccgctcggt gcgaacgcgg gggcgcccgc ggcgggaggc gcctcgttcg accgcgcagc
     8641 gtggcagcgc cttcaggacg cccaggccgc cgcgaccgcg aagcccgacg actacgacgc
     8701 gctcgtccag ctcgggaatg ccgcctacga cgcgcacgag tacgcgcagg cgatcgaagc
     8761 ctacgagcga gcgctgaagg tccggcccgg cgacccgaac gtgatgaccg acctcggcac
     8821 ggcctaccgg aacgagggta agctcgacga ggcgctctcg ctcttccgca aggcgcgggc
     8881 cgcgaacccg agccactggc agtcgctcta caacgaggtc gtcgtcctcg cgctcgacaa
     8941 gcacgagcac gacgcggccg acgccgcgct cgcgcggctg aagaaggagc acccgggtct
     9001 gccggcgatc gagaacctcg agaggcagat cggcgccgct cactgaggat cgtgaagccg
     9061 cggagcggag acctcttcga ggagccgatc ccggaccggc gggcgacgcc ttccgtggcg
     9121 ccgctcgccg aacgtttccg cccgcgcgac ctctcggaga tgcggctccc cgcgtcggtc
     9181 gccgggccgt cgtccctcct cggccgcgcg atcgaggcgg accgggtccc gtccctcgtc
     9241 ctctggggac cgccgggaac ggggaagacg acgctcgcgc gggtcatcgc gacccggacg
     9301 aaggcccgct tcctctcctt ctcggccgtc gtctcgggcg tgaaggagat ccgggaggtc
     9361 ctcgaggagt cgcagcgtct ggccacgcgc ggcgagagga ctctcctctt cgtcgacgag
     9421 atccaccgct tcaaccgggc ccagcaggac gtcttcctcc cgtacgtcga gtcgggcgcg
     9481 gcgacgctcg tcggcgccac gacggagaac ccgtcgttcg agctgaacgg cgccctcctc
     9541 tcgaggatgc gcgtcgtcgt cctcccgccc ctgtcgcgcg aagacctcga gtcgatcctc
     9601 gggagcgccg cggcggacgc cgagcggggc ctcggggggc gtgtgacgct cggcgagggg
     9661 gccgtcgact ggctcgtcgg cttctccgac ggagacgcgc ggcgcgcttt gaacgcgctc
     9721 gagacggcgg ccgaccacgc gggagagggg gccgtcctga cggcggcgct cctcggcgag
     9781 atctacgcgc ggaagtcgct cctcttcgac aagtcgggcg aggagcacta caacctcatc
     9841 tccgccctcc acaagtcggt gcgcgactcc gacgtcgacg cttcgctcta ctggctcgcg
     9901 cggatgctgg aggcggggga ggacccgctc ttcgtcgcgc ggcggctcgt gagggcctcg
     9961 acggaggacg tcggcctcgc cgacccgaac gccctccggc tcgccatggc cgcccgcgac
    10021 gccgtccact tcctcgggat gcccgagggg gcgctcgccc tcgcggaggc gaccgtctac
    10081 ctcgcgctcg cgccgaagtc gaacgcgctc tatgccgcct acggcgaggc ggcccgcgac
    10141 gtggccgaga cgccgaacgc gccgccgccc gccgcgatcc tgaacgctcc gacgaggctg
    10201 atgaaggacg tcggctacgg gaaggggtac gtctacgccc acgacacggc ggaggggacg
    10261 gccggcctct cgtgcctccc cgacgccctc gccggccgcc gctactaccg gccgaagggg
    10321 acgggacggg aggccgaggc gaagcgccgg ctcgacgagc tccgcgagct tcgcgagcgg
    10381 ctccggcggg agaggcaccg ccgctgaccg ccccgcggta acctttcccc atgctcgacc
    10441 tcgaagcgat ccgcg
//
LOCUS       DAOTEZ010000107        10379 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4524_length_10379_cov_6.638706, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000107 DAOTEZ010000000
VERSION     DAOTEZ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10379
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4524_length_10379_cov_6.638706"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="P5164_07450"
     CDS             <1..398
                     /locus_tag="P5164_07450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRY43767.1"
                     /translation="MPAIAVVALAFVAAGALGADGVRRREVVPGPPAPSGTPALAAAL
                     ASFEAPAEMTFPQAPDSPGPVSFRHASHVDASAPDCLACHGNSYSFLGRTEAPPAGLM
                     HDTKHCGGCHDGTSAFSIEDDCTACHKSE"
     gene            416..2023
                     /locus_tag="P5164_07455"
     CDS             416..2023
                     /locus_tag="P5164_07455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY43768.1"
                     /translation="MQRLRANSLAVRLTGTLVAALAALLLVSWFVSLSLQRRFSESTA
                     RYSALALSETLTGGLHGMMLANDREGLESSVRAVAKAQPNIRVRVFNKEGSIVYSSIP
                     EEAGRKVDTTSEACFKCHAEGQPIEKLPPGDRTRTFQLDGVRTLGVIRPIENEPSCST
                     AACHAHPPEKRLLGVVDVSIQMVRQEQVRRQTMALMAVTGVATLLLVAAVVVLVVRRA
                     VHRPVRHLADVLGALGGGDYTARYDDEPIAEFAYLGHHVNRMAGDLQKANAEIVDWAQ
                     TLERRVEEKTGELKAAQAQMLRVERMASLGKLAAVVAHEINNPLASVVTYSKLLLRRF
                     TQKGGPKPGDDSEKILEAIASESARCGEIVSNLLLFARRTGSRMEPTDLNKLVDRSLF
                     LLKHKMDLAQVATAEELAPDLPQVLCDPSQIEQAILALAINAIEAMPGGGTLSIRTAP
                     HGERGARIEIADTGVGMEEEVKRQIFEPFFTTKGDGEGKGLGLGLAVVYGIVQRHGGA
                     IDVESAPAAGTRFVLTLPGTATPGEES"
     gene            2023..3363
                     /locus_tag="P5164_07460"
     CDS             2023..3363
                     /locus_tag="P5164_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRY43769.1"
                     /translation="MSAPVKLMIVDDEPHLRSSLTFWFKEEGYDVLSAGSGREALATL
                     LREAPQILLVDIKMPGMDGLEVLRKVRETAPETTVIIMTAYASVETAIQALKDGAYDY
                     IVKPFDPEAISRLVKKAAERYTLLSENRELKARLEAAEPALVTAGGGPMAKVLELVDQ
                     VAPTDTSVLVTGESGTGKELVARLIHARSPRRFGPLSVVNCGALSEGVLESELFGHEK
                     GAFTGAVGRRRGKVELAHEGTLFLDEIGDVPPKVQVDLLRVLQEKQVVRVGGNQTIPV
                     DFRLVTATHRDLEDEVAAGRFRQDFFFRIHVFAIELPSLRDRPADIPVLAQHFLARFA
                     SAMNRRIGGFSPGALAAMADYPWPGNVRELQNAIERAVVLCKGDTIEAAHFPFTTNGH
                     AAAATSLTLASAEEAHIRKVLATMSFNVAQAARALEIDRVTLYNKMRKYGIDRP"
     gene            3360..3872
                     /locus_tag="P5164_07465"
     CDS             3360..3872
                     /locus_tag="P5164_07465"
                     /inference="COORDINATES: protein motif:HMM:NF019610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M54"
                     /protein_id="HRY43770.1"
                     /translation="MKVRLLHTRDVSPGHVRSAFSGLAPPLVPGEALPFRASLSSAYD
                     RSRAQYDAFSVLEAVPEPEAGWLNLLLVGVDLFVPALTYVFGLSHVGGRRGVLSLSRL
                     KPPEESAVSEELLLRRLAIEVAHELGHAAGLVHCAVGDCAMHRSLWPESIDLKNPAYC
                     LSCRALFPEG"
     gene            complement(3892..4875)
                     /locus_tag="P5164_07470"
     CDS             complement(3892..4875)
                     /locus_tag="P5164_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HRY43771.1"
                     /translation="MKAMLLDAPRQALRLAEIPDPEPGPGQLLLRVRACALCRTDLHV
                     VDGDLREPKLPLVLGHEIVGVVERAGEGGSLFAPGDRVGVPWLGVTCGRCRYCLEGAE
                     NLCDEARFTGYHRDGGFAELAVADERFCFPIPAELGDVEAAPLLCAGLIGHRTLRMAG
                     DPQKVGIWGFGAAAHLVAQVARYEGREVFAFTRPGDSWAQEFARSLGAVWAGGSDELP
                     AEPLDAALVFAPIGALVPAALRSIRKGGVVVCGGIHMTDIPSFPYELLWGERVVRSVA
                     NLTRRDGEEFLAVAARAGVKSQVRAYPLEAANEALEDLREGRLEGVAVVVP"
     gene            complement(5043..5117)
                     /locus_tag="P5164_07475"
     tRNA            complement(5043..5117)
                     /locus_tag="P5164_07475"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5083..5085),aa:Gly,seq:gcc)
     gene            complement(5187..8561)
                     /locus_tag="P5164_07480"
     CDS             complement(5187..8561)
                     /locus_tag="P5164_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43772.1"
                     /translation="MRRSERRFSMRSWRKAALVAVAAVLGLAPSGAEAGLRLEGDGGA
                     GVDVRAFASGAGGFWAATRSGVLRGTSLDTAWRFDGLSGKPVSSVATLGDEVFAATGE
                     ELWRRSANGSWSLEALPSSVAFPTTLAVDAAGTLWVGGLGVWRRSGAAWTAAPSPGPG
                     IVTAMLADPAGLVVGLSTGSAARWNGSGWAALNGGVGPGEAIRALASFGGTLWAGTNV
                     TLYWWGVGAWVADGPFGGHDVRALSTWGGALRAATADAGVLVRSGSAWTPASTGILPR
                     GVQAFLVSGSELLAGTAGGPVYRFWNGIWHPSPVTPLGAGVAADAFAFPAESAGSQVG
                     AAVGATRGGGAGYLLRPCCLPGADHPELPSLPDGCGDATAVAPTASAQPDVVVATSCG
                     PYLLSWMSATPAGSGLPARALPTKLAAHGGAVFGGTAGSGLWRFDGSSWRTDPVGGNV
                     GTDGVGTGTVQAIRSLGGALWVGMAEGLYARGSGPWTEVSAGLPSAGFVSALAGDAST
                     AFAGLATGGVYRRPAGKPFQKDAAGLNSAPVASLDLAGGRLWAAAGSRGLAVKRDGGW
                     APENGGLPPNAVVTVVRGGVNPDSDGAALVVGTDGDGFYRADLFAKARTLPVVLDVVG
                     QGGARFLSELVVGSRGATQRTCTVTFTPSPGFAGEAGDLTPKSATLTLPAGRELRAAD
                     ALAFLRSLGLPVPVATAAAPVAGSVSLRSTTPGVVDEAYLVARTYTRGASGGTFGLFY
                     DAPSDLDAAEEVATVYGLRSVAGEARSNLAAVHLPGRGSEPIELSVQVYGQDGVPAGD
                     PLVRSLAPGEWTQWNGVLGLAGLPEGSYGYARVRRTKGEGAFAAYGVVNDARTSDGSY
                     LPAFRPGGLSSVRTLVVPVVLDVAGEAGSRYTTEVTIVNDGTIATPVDLVYRPAPGFG
                     TVGGVAQVTLELAARQQVTLPDVLAYLRAHGMQIPDGATAPQAGTLGVTFRWLTELDA
                     PSTVVLARTSTPNPDTAVGGTFGLFYPAVAKGGGARTTALVPGLAQDEGVRSNLAVVN
                     VGGGSELPVTLEARLHDADTGAPVGSPLTVRLAPGDWHQWSRVREAAGAPATTKRFTA
                     VVRRTAGDDTFLAYGVLNDAVTSDGSYQTMIPADLY"
     gene            complement(8548..8994)
                     /locus_tag="P5164_07485"
     CDS             complement(8548..8994)
                     /locus_tag="P5164_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRY43773.1"
                     /translation="MPSGWKERLSELGISRRRRPAAAKPVPTRPATTPKGRAGEDEAA
                     RFLEEQGLELLARNVRAGGGELDLVAADGRCVVFVEVKWRRDASRGTPAEAVTPVKRR
                     RLLSAARAWLAANPSGAPRDVRFDVVAIEGTAGTIDWIRGAFDATE"
     gene            9015..9311
                     /locus_tag="P5164_07490"
     CDS             9015..9311
                     /locus_tag="P5164_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43774.1"
                     /translation="MGDVFVIQCPDCDSTLKVDRETGAVLSHEPAAAKRKLGSIEEAA
                     AEIARRKERAEDLFAANVEREKHKSEILEKTFREALQKAQKEPPGRPRSPFDAD"
     gene            complement(9372..9800)
                     /locus_tag="P5164_07495"
     CDS             complement(9372..9800)
                     /locus_tag="P5164_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43775.1"
                     /translation="MADPKKPDRKAPSNPYVTGPTRILDASSSGPSARGGGEATRPGV
                     RPELVTDGMKAHRREIEKRKRQASIEKMHLEMQVADERKAIWKWTFRVVLVLVILFAY
                     RKMQDAYGNQWPMWETWIVLAVVLFVGIGWLLWYLNKTEL"
     gene            complement(9822..>10379)
                     /locus_tag="P5164_07500"
     CDS             complement(9822..>10379)
                     /locus_tag="P5164_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRY43776.1"
                     /translation="QQNVETLNGYGVKRIVTNCPHCFNTLKNEYPDFGGAFEVIHGTE
                     LVALLLAEGRLQLTESVAKSISFHDACYLGRHNDVYDAPREVLAAIPGLTLTELPRTG
                     KNGLCCGAGGGRMWLDEKIGTRINQLRYEEIEAAGTDLVGVSCPFCMVMLGNAQTEMS
                     GKTQPFDVLELAAQALPAHPGAGAS"
BASE COUNT         1488 a         3990 c         3591 g         1310 t
ORIGIN      
        1 cgatgcccgc gatcgccgtc gtcgcgctcg cgttcgtcgc ggccggcgcc ctcggcgccg
       61 acggcgtgcg ccgccgcgag gtcgttcccg gcccgccggc gccttcgggg accccggccc
      121 tcgccgccgc gctcgcgagc ttcgaggcgc ccgccgagat gaccttcccc caggcgcccg
      181 acagccccgg gcccgtctcg ttccgccacg cctcccacgt cgacgcctcc gcccccgact
      241 gcctcgcctg ccacgggaac agctactcgt tcctcggccg gacagaggct cccccggcgg
      301 gcctgatgca cgacacgaag cattgcggcg gatgccacga cggcacgtca gcgttctcga
      361 tcgaggacga ctgcacggcc tgccacaagt ccgagtagga aggacgggag cggcgatgca
      421 gaggctccgg gccaactccc tcgcggtccg cctgacgggg accctcgtcg ccgctctcgc
      481 cgccctcctc ctcgtctcct ggttcgtgtc gctctccctg cagaggcggt tctccgagtc
      541 gaccgcgcgc tacagcgcgc tcgccctctc ggagacgctc acgggcgggc tccacgggat
      601 gatgctcgcg aacgaccgcg aggggctcga gagctcggtg cgggccgtcg cgaaggccca
      661 gccgaacatc cgcgtccgcg tcttcaacaa ggaaggctcc atcgtctact cgtcgatccc
      721 ggaggaggcg ggccggaagg tcgacaccac ctccgaggcc tgcttcaagt gccacgccga
      781 ggggcagccg atcgagaagc tccctccggg cgaccggacc cgcacgttcc agctcgacgg
      841 cgtccggacg ctcggcgtca tccggccgat cgagaacgag ccctcgtgct cgacggccgc
      901 ctgccacgcg cacccgcccg agaagcggct cctcggcgtc gtcgacgtct ccatccagat
      961 ggtgcggcag gagcaggtgc ggcgccagac gatggccctg atggccgtga ccggcgtcgc
     1021 cacgctcctc ctcgtcgccg ccgtcgtcgt cctcgtcgtg aggcgggccg tccaccgccc
     1081 cgtccgccac ctcgccgacg tcctcggcgc tctcggcggc ggcgactaca ccgcccggta
     1141 cgacgacgag ccgatcgccg agttcgccta cctggggcac cacgtgaacc ggatggcggg
     1201 cgacctgcag aaagcgaacg ccgagatcgt cgactgggcc cagacgctcg agcgccgcgt
     1261 cgaggagaag accggcgagc tgaaggcggc ccaggcccag atgctccgcg tcgagcggat
     1321 ggcctccctc ggcaagctcg cggcggtcgt ggcgcacgag atcaacaacc cgctcgcgag
     1381 cgtcgtcacc tactcgaagc tcctcctccg ccgattcacc cagaagggag ggccgaagcc
     1441 gggggacgac agcgagaaga tcctcgaggc gatcgcctcc gagtcggccc gctgcggcga
     1501 gatcgtctcg aacctgctcc tcttcgcgcg ccgcaccggc tcgcggatgg agccgaccga
     1561 cctgaacaag ctcgtcgacc gctccctctt cctcctcaag cacaagatgg acctcgccca
     1621 ggtcgccacc gccgaggagc tcgccccgga cctcccgcag gtgctctgcg acccgtccca
     1681 gatcgagcag gcgatcctcg cgctcgcgat caacgcgatc gaggcgatgc ccggcggcgg
     1741 gacgctctcg atacgcaccg cgccgcacgg ggagcgcggg gcgcggatcg agatcgccga
     1801 caccggcgtc gggatggagg aggaggtcaa gcggcagatc ttcgagccct tcttcacgac
     1861 gaagggggac ggcgagggga aggggctcgg cctcggcctc gcggtcgtct acggcatcgt
     1921 ccagcggcac gggggcgcga tcgacgtcga gagcgccccc gccgccggca cccgcttcgt
     1981 cctgaccctg ccggggaccg ccaccccggg agaggagtcc tgatgtccgc gcccgtcaag
     2041 ctgatgatcg tcgacgacga gccgcacctg cggagctcgc tcacgttctg gttcaaggag
     2101 gaggggtacg acgtcctctc cgccgggagc gggcgcgagg cgctcgcgac gctcctgcgc
     2161 gaggcgccgc agatcctcct cgtcgacatc aagatgcccg gcatggacgg gctcgaggtc
     2221 ctccgcaagg tgcgcgagac cgctcccgag acgaccgtca tcatcatgac ggcctacgcc
     2281 tccgtcgaga ccgcgatcca ggccctcaag gacggggcgt acgactacat cgtcaagccg
     2341 ttcgacccgg aggcgatctc gcgcctcgtg aagaaggcgg ccgagcgcta cacgctcctc
     2401 tcggagaacc gcgagctgaa ggcgaggctc gaggcggccg agccggccct cgtcacggcg
     2461 ggcggcgggc cgatggcgaa ggtcctcgag ctcgtcgacc aggtcgcgcc gaccgacacg
     2521 agcgtcctcg tcaccggcga gagcggcacc ggcaaggagc tcgtcgcgcg cctcatccac
     2581 gcccggagcc cccgccgctt cggcccgctc agcgtcgtca actgcggcgc cctctccgaa
     2641 ggggtgctcg agagcgagct cttcggtcac gagaaggggg ccttcacggg ggccgtcggc
     2701 cggcgccgcg gcaaggtgga gctcgcccac gaggggacgc tcttcctcga cgagatcggc
     2761 gacgtcccgc cgaaggtcca ggtcgacctc ctccgcgtcc tccaggagaa gcaggtcgtc
     2821 cgcgtcggcg ggaaccagac catcccggtc gacttccgcc tcgtcaccgc gacccaccgc
     2881 gacctcgagg acgaggtcgc cgcgggccgc ttccggcagg acttcttctt ccggatccac
     2941 gtcttcgcga tcgagctccc gtcgctgcgg gatcgcccgg ccgacatccc ggtcctcgcg
     3001 cagcacttcc tcgcgcgctt cgcgagcgcc atgaaccgcc ggatcggcgg cttctcgccc
     3061 ggcgcgctcg ccgcgatggc cgactacccc tggccgggga acgtccggga gctccagaac
     3121 gccatcgagc gggccgtcgt cctctgcaag ggggacacga tcgaggcggc ccacttcccg
     3181 ttcacgacga acggccacgc cgccgctgcg acctcgctga cgctcgcctc cgccgaggag
     3241 gcccacatcc ggaaggtcct cgcgacgatg tcgttcaacg tcgcccaggc cgcccgcgcc
     3301 ctcgagatcg accgggtcac cctctacaac aagatgcgga agtacggcat cgaccgcccg
     3361 tgaaggtccg gctcctccac acccgagacg tctcgccggg gcacgtgagg agcgccttca
     3421 gcggcctcgc ccccccgctc gtcccgggcg aggcgctccc cttccgcgcg tccctctcct
     3481 ccgcgtatga ccgcagccgg gcccagtacg acgccttttc ggtcctcgag gccgtccccg
     3541 agcccgaggc cggctggctg aacctcctcc tcgtcggcgt cgacctcttc gtccccgccc
     3601 tcacctacgt cttcggcctc tcccacgtcg gcgggcgccg gggcgtcctc tccctgtcgc
     3661 ggctcaagcc gcccgaggag tccgccgtct ccgaggagct cctcctccgg cgcctggcga
     3721 tcgaggtcgc ccacgagctc gggcacgcgg ccggcctcgt ccactgcgcc gtcggcgact
     3781 gcgcgatgca ccgctccctc tggccggaga gcatcgacct gaagaacccc gcctactgcc
     3841 tctcctgccg cgccctcttc ccggagggat agaccggcgc cgcgggcgtc gtcaggggac
     3901 gacgacggcg accccctcga ggcgcccctc gcggaggtcc tcgagcgctt cgttcgccgc
     3961 ctcgagcggg taggcgcgca cctggctctt cacccccgcc cgcgccgcga cggccaggaa
     4021 ctcctccccg tcccgccgcg tcaggttcgc gacggagcgg acgacgcgct cgccccagag
     4081 gagctcgtaa gggaacgacg ggatgtcggt catgtggatg ccgccgcaga cgacgacgcc
     4141 ccccttgcgg atcgagcgga gggcggccgg caccagcgcc ccgatcgggg cgaagacgag
     4201 cgcggcgtcg agcggctcgg ccgggagctc gtcggagccg ccggcccaga ccgcgccgag
     4261 ggagcgggcg aactcctgcg cccaggagtc gccgggacgg gtgaacgcga agacctcgcg
     4321 cccctcgtac cgggccacct gcgcgacgag gtgcgcggcg gccccgaagc cccagatccc
     4381 gaccttctgc gggtcgcccg ccatccggag cgtccggtgc ccgatgaggc cggcgcagag
     4441 gagcggcgcg gcctcgacgt ccccgagctc ggcggggatc ggaaagcaga accgctcgtc
     4501 ggccacggcg agctcggcga agcctccgtc gcggtggtag ccggtgaacc gcgcctcgtc
     4561 gcagaggttc tccgctcctt cgaggcagta ccggcagcgc ccgcaggtca cgccgagcca
     4621 cgggacgccg acgcggtcgc ccggggcgaa gagcgagccc ccttccccgg cccgctcgac
     4681 gacgccgacg atctcgtggc cgaggacgag agggagcttc ggctcccgga ggtccccgtc
     4741 gacgacgtgc aggtccgtcc ggcagagggc gcacgcccgg acgcgcagaa ggagctggcc
     4801 cggccccggc tccgggtccg ggatctccgc gagacggagc gcctgacggg gagcgtcgag
     4861 cagcatcgct ttcacgggtc cctcctcgca cgacgggagc gtcccggcgg tccggccggc
     4921 cacagtcccc gtccctcggg atcgagacta caggagcggc ctcggcccgc gcgagcgggg
     4981 tcgcccccga aaacgaaaac tcccgcaaac cctgcgaagg acctgcggga gaatgagttc
     5041 gatggagcgg gacacgaggg tcgaactcgc gacatctacc ttggcaaggt agcgctctac
     5101 cactgagcta gtcccgctca cgggaccggc ggaagatagc gaagaagggc gttctcgtca
     5161 aggcgtgccg cgccgccgac tgcggttcaa tagaggtccg ccgggatcat cgtctggtag
     5221 ctcccgtcgg aggtgacggc gtcgttcagg acgccgtagg cgaggaacgt gtcgtccccc
     5281 gcggtccgcc gcacgaccgc cgtgaaacgc ttcgtcgtcg ccggcgcgcc ggccgcctcc
     5341 cggacccgcg accactggtg ccagtcgccg ggcgcgagcc gcaccgtgag cgggctgccg
     5401 accggggcgc ccgtgtcggc gtcgtggagc cgcgcctcga gcgtgaccgg cagctccgag
     5461 cccccgccca cgttcacgac cgcgaggttc gagcggacgc cctcgtcctg ggcgaggccc
     5521 gggacgagcg ccgtcgtccg ggcgccgcct cccttcgcga cggccgggta gaagaggccg
     5581 aacgtgccgc cgacggccgt gtccgggttc ggcgtcgagg tgcgcgcgag gacgaccgtc
     5641 gacggcgcgt ccagctccgt cagccagcgg aacgtcacgc cgagggtgcc ggcctgcggc
     5701 gccgtcgcgc cgtcggggat ctgcatcccg tgggcgcgca ggtacgcgag gacgtcgggg
     5761 agcgtcacct gctgccgggc cgccagctcc agcgtcacct gcgcgacgcc gccgaccgtc
     5821 ccgaagccgg gcgctggccg gtagacgagg tcgaccggcg tcgcgatcgt cccgtcgttc
     5881 acgatcgtca cctcggtcgt gtagcgcgag cccgcctcgc ccgcgacgtc gaggacgacc
     5941 gggacgacga gggtcctcac ggacgacagg cctcccgggc ggaacgccgg gaggtagctg
     6001 ccgtcggagg ttcgcgcgtc gttcacgacg ccgtaggccg cgaacgcacc ctcccccttc
     6061 gtccggcgga cccgcgcgta gccgtagctc ccttcgggaa ggccggcgag gccgaggacg
     6121 ccgttccact gcgtccactc ccccggcgcg agcgagcgga cgagcggatc gcccgccggg
     6181 acgccgtcct ggccgtagac ctggacggag agctcgatcg gctcgctccc gcgccccggc
     6241 aggtggacgg cggcgaggtt cgagcgggcc tcgccggcga ccgaccggag gccgtagacc
     6301 gtcgcgacct cctccgccgc gtcgaggtcg gacggggcgt cgtagaagag gccgaaggtc
     6361 ccgcccgagg cgccccgcgt gtaggtgcgc gccacgaggt acgcctcgtc gacgaccccg
     6421 ggcgtcgtcg accggagcga gacgctgccg gcgaccggcg cggcggccgt ggcgacgggg
     6481 accggaaggc cgagagaacg aaggaacgcg agcgcgtcgg cggcgcgcag ctcgcgcccc
     6541 gcggggagcg tcagcgtcgc cgacttcggc gtgaggtcgc ccgcttctcc ggcgaagccc
     6601 ggcgacggcg tgaacgtgac ggtgcacgtc ctctgcgtcg caccgcgcga cccgacgacg
     6661 agctccgaga ggaatcgcgc gcccccctgg ccgacgacgt ccaggacgac ggggagcgtc
     6721 cgcgccttcg cgaagagatc cgcgcggtag aagccgtcgc cgtccgtgcc gacgacgagc
     6781 gccgcgccat cggagtcggg attcacgcct ccgcggacga cggtgacgac ggcattcggc
     6841 ggcagcccgc cgttctcggg agcccagccc ccgtcccgct tcacggcgag cccgcggctg
     6901 ccggccgcgg cccagagccg cccgccggcg aggtcgagcg aggcgaccgg cgccgaattg
     6961 aggcccgccg cgtccttctg aaacggcttt cccgccggcc gtcggtagac gccgccggtc
     7021 gccagccccg cgaacgcggt cgacgcgtcc ccggcgagcg cggagacgaa gccggccgac
     7081 gggaggccgg ccgagacctc ggtccacggc ccgctgccgc gcgcgtagag cccttcagcc
     7141 atgccgaccc agagcgcgcc gccgagcgac cggatcgcct ggacggttcc cgtcccgacg
     7201 ccgtcggtgc cgacgttgcc tccgacggga tcggtcctcc acgaggagcc gtcgaaccgc
     7261 cagaggccgc ttccggccgt ccctccgaag acggctccgc catgagcggc gagcttcgtc
     7321 gggagggccc gggccgggag gcccgacccg gcgggagtcg ccgacatcca gctcagcagg
     7381 tacggcccgc agctcgtggc gacgaccacg tcgggctgcg ccgacgccgt cggcgccacc
     7441 gccgtcgcgt cgccgcagcc gtcggggaga gagggaagct ctggatggtc ggcgcccggc
     7501 aggcagcagg gccggagcag gtagcccgcc cctccgccgc gcgtcgcgcc gaccgccgcg
     7561 ccgacctgcg aaccggccga ctccgccgga aacgcgaagg cgtccgccgc cacgccagcc
     7621 ccgagcgggg tgacggggga cggatgccag atcccgttcc agaagcggta gacggggccg
     7681 cccgcggtcc cggcgagaag ctcggagccg gaaacgagga acgcctgcac cccgcgcggc
     7741 aggatgcctg tcgaggccgg cgtccaggcg ctccccgacc ggacgaggac gcccgcgtcg
     7801 gccgtcgcgg cgcggagcgc gccgccccac gtcgagaggg cgcggacgtc gtgcccgccg
     7861 aacgggccgt ccgcgaccca cgccccgacc ccccaccaat agagggtcac gttcgtcccg
     7921 gcccagagcg tcccgccgaa tgacgcgagc gcgcggatcg cctctcccgg gccgacgcca
     7981 ccgttgaggg cggcccagcc cgagccgttc catcgggccg cgctccctgt cgagaggccg
     8041 acgacgaggc ccgcggggtc cgcgagcatc gccgtgacga tcccggggcc cggcgacggc
     8101 gcagccgtcc acgcggcacc ggagcgccgc cagacgccga gcccgccgac ccagagcgtt
     8161 cccgccgcgt cgaccgcgag cgtcgtcggg aaggcgacgc tcgacgggag cgcctcgagg
     8221 ctccagctcc cgttcgcgga gcgccgccag agctcttccc ccgtagccgc gaagacttcg
     8281 tccccgagcg tcgcgaccga cgagaccggc ttcccggaga gaccgtcgaa gcgccacgcc
     8341 gtgtcgaggg aggttccccg gaggaccccg cttcgcgtcg cggcccagaa tccgcctgct
     8401 cccgacgcga acgcgcggac gtccaccccg gcgccgccgt ccccctcgag ccgaagcccc
     8461 gcctcggccc ccgaaggagc cagtccgagg accgccgcca ccgcgacgag cgccgccttc
     8521 cgccagctcc gcatcgagaa cctccgctca ctccgtcgca tcgaacgctc ctcggatcca
     8581 gtcgatcgtt ccggccgtcc cctcgatcgc cacgacgtcg aacctcacgt cgcgcggcgc
     8641 gccggacggg ttcgccgcga gccaggcgcg ggcggccgag aggagccgcc tcctcttcac
     8701 gggagtgacg gcctccgccg gcgtcccacg gctcgcgtcc ctccgccact tcacctcgac
     8761 gaagacgacg cagcggccgt ccgccgccac gaggtcgagc tctccccctc ccgcgcggac
     8821 gttccgcgcg aggagctcga gcccctgctc ctcgaggaag cgggccgctt cgtcctcgcc
     8881 ggcgcgcccc ttcggcgtcg tcgcgggtcg cgtgggaacc ggcttcgcag cggccgggcg
     8941 ccgccggcgc gagatcccga gctccgagag gcgctctttc caccccgacg gcatcggtcg
     9001 aggataatcg acccatgggc gacgtcttcg tgatccagtg tcccgactgc gactcgacgc
     9061 tgaaggtcga ccgggagacc ggagcagtcc tctcccacga gccggccgcc gcgaagcgga
     9121 agctcggatc gatcgaggag gcggccgcgg agatcgcccg ccgcaaggag cgcgcggagg
     9181 acctcttcgc cgcgaacgtc gagcgggaga agcacaagag cgagatcctc gagaagacgt
     9241 tccgcgaggc gctccagaag gcccagaagg agccgccggg ccggccccgc agccccttcg
     9301 acgccgactg acctcagccg gaccgccggc tcaggcgcga gatccggagg aacggcgttc
     9361 ccccgcgatc attacagctc ggtcttgttg aggtaccaga gcagccagcc gattccgacg
     9421 aagaggacga cggccaggac gatccacgtc tcccacatcg gccactggtt cccgtaggcg
     9481 tcctgcatct tccggtaggc gaacaggatc acgaggacga ggaccacccg gaaggtccac
     9541 ttccagatcg ccttccgctc gtcggcgacc tgcatctcga ggtgcatctt ctcgatcgag
     9601 gcctggcgct tgcgcttctc gatctcgcgc cggtgcgcct tcatcccgtc ggtgacgagc
     9661 tcggggcgca cccccgggcg cgtcgcctct ccgcctcccc gggcggaagg gccggacgaa
     9721 gaggcgtcga ggattcgcgt cgggcccgtg acgtacgggt tcgacggcgc cttgcggtcg
     9781 ggcttcttcg gatccgccac gtcgcctcct caggattccc gtcagctcgc cccggcgccc
     9841 gggtgggccg gaagggcctg cgcggcgagc tccaggacgt cgaacggctg ggtcttcccc
     9901 gacatctccg tctgggcgtt cccgagcatc accatgcaga acgggcacga gactccgacg
     9961 aggtccgtcc cggccgcctc gatctcctcg tagcggagct ggttgatccg ggtcccgatc
    10021 ttctcgtcga gccacatccg cccgccgccg gcgccgcagc agaggccgtt cttcccggtc
    10081 cgcggcagct ccgtcagcgt caggccgggg atcgccgcga ggacctcccg cggcgcgtcg
    10141 tagacgtcgt tgtgccggcc gaggtagcag gcgtcgtgga aggagatcga cttcgcgacg
    10201 ctctccgtca gctgcaggcg accttcggcc aggagaaggg ccacgagctc ggtcccgtgg
    10261 atcacctcga acgccccccc gaagtcgggg tactcgttct tcagcgtgtt gaagcagtgc
    10321 gggcagttcg tgacgatccg cttcacgccg tagccgttca gcgtctcgac gttctgctg
//
LOCUS       DAOTEZ010000108        10309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4563_length_10309_cov_6.225571, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000108 DAOTEZ010000000
VERSION     DAOTEZ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10309
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4563_length_10309_cov_6.225571"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2517
                     /locus_tag="P5164_07505"
     CDS             <1..2517
                     /locus_tag="P5164_07505"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRY43777.1"
                     /translation="LGVSEAGGAFTIPLVGQGLKTGPNLPLDVVSTVSAFELHGVGSG
                     VYGQIRPWAGAASSIPASGFGPDATVWFGIGGTSGWLSPSESQYTIEIDRNGDLTPDA
                     TVTTMPTSDVPSPDDPNVRSAPTDVIVAGTSIARGAAAKIYRYVNLDPALRDTNLFGS
                     NVLVVPAPAGENGLGLSAGNARFRYRITRSSRLFPVTGTDETAWYTFDAEKPGYSVLA
                     ADGTPFHDDLGGTALPARYDPAAAQAAGALGVLLLHHHNPKSSRIETLTERNTPPTVS
                     IAAPTPQTAFIPAGLGLHFHAEGSDPDAGDMLSYLWDLCDGRKKTGALVTASFPRAGS
                     CTVRVTVTDGAGATATAQVVVTVEEPEPDLGLSKLLPVVLDVQGVGGAPFTTELTLVS
                     RMPALTTVLLRYTAAVGSGTGWAGVGLEGRGTRVIPDVIAYLRRQGLPIPDDGTNQVG
                     TLEVMPLGATKPSDYFVGARTSTPGDGGSFGLFYADARTATATAVVAGLQENDAMRSN
                     LAVVNAGAEPVTLAIRLLGPLGEDLGALEDATLPAWGWKQFDRPLLGKAASGRALVAR
                     IAGSSPFSAYGVLNDAGTSDGSYIPPHLPGAAGPADLMIPVVLDVQGLGTSRYATEVT
                     LANLGTTPLALTLAYGATAEFGGGSGSVPLTLAAGEQRILQDVLSFLRAGGLEIPGDG
                     ANVAGSLLVKAPAGASSGALVAGARIHTRSTLRPGTFGVYYPGLSADESADGPVSVNG
                     LRQDASMRSNVALVNVGDAGVATLRITFFSESGQPLERPEERTLNAGEWLQLGQPLAT
                     RGATAGSARIERIRGRSRFLAYGVLNDAATSDGSYLPMSR"
     gene            2852..3211
                     /locus_tag="P5164_07510"
     CDS             2852..3211
                     /locus_tag="P5164_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43778.1"
                     /translation="MSRTVRSALLLAAALLPLAVQAGAVIDLLDASREAHPATLVELL
                     VHGHTHGRKVVSHAHAAPGDRDRGPRLGAPDQVATLPTTAGAMTAVASSTRYPHEPAA
                     VGSSLPPKFLLHASLLR"
     gene            3308..5878
                     /locus_tag="P5164_07515"
     CDS             3308..5878
                     /locus_tag="P5164_07515"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019499677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRY43779.1"
                     /translation="MERTPELPARVDVVRPSGLTEEEAARRLAREGPNELPASKRRGP
                     FRIALEVVKEPMFLLLIACGSLYLLLGDVGEAFLLLGFVFVIVGITFVQERKTERALD
                     ALRDLASPRALVVRNGEKRRIAGRDVVRGDVLLLSEGDRVPADAVVRRATSLSVDESL
                     LTGESVPVGKQPAAGAAPAAAPGGDDTPFVFSGTLVVKGQGVAEVVAVGAATEMGKIG
                     RALAVLEPEDTPLQRQTARLVRLFALIGFGLCATVVAAYALTRGDFLKGLLAGLTLGM
                     AMLPEEFPVVLTVFLALGAWRISRHHVLTRRIPAVETLGAATALCVDKTGTLTMNRMS
                     VAELAVGDEVLAVDPKGASLPEDFHELVELAILASPADPFDPMEKAMRELGERTLAGT
                     EHLHGDWELLREYPLSRQILAMSRVWRSPDGSEFVIAAKGAPEAIADLCHLGPEETAR
                     LERRIADLASRGRRVIGVARAAFRGRALPAGQHDFGFRFVGLLALSDPVRPGVPEAIA
                     ECRRAGIRVLMITGDYAGTARSIAREIGLADPETVVTGPELDTLDDAALAARVREACV
                     FARMVPEQKLRLVSALKANGEIVAMTGDGVNDAPALKAAHIGIAMGGRGTDVAREAAA
                     LVLLDDAFESIVKAVRTGRRIFDNLRKAMLYIFAVHVPIAGLSLFPVLLGWPLALLPI
                     HIVFLELIIDPACTVVFEAEEGEEGVMRRPPRPLDEPIFGGRGLLLGLVQGLGELVVD
                     LALYGWALAAHGEPVARALAFTTMVLGNVGLIFLNRAGSRSLLETLRAPNRPERWVTG
                     GAFAFLALALGVPGARELFGFATPHPQLLALSAAGALVSLAVANLARHLLAERTATGR
                     "
     gene            5882..6457
                     /locus_tag="P5164_07520"
     CDS             5882..6457
                     /locus_tag="P5164_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43780.1"
                     /translation="MALRFTLTRLLPSVALFLALLGGALLADLVLHRAGLAGVGRWGG
                     LAGTVLLAASFLYSARKRKLLFAGSPKGLLRLHETLGWAGALLVLVHGGIHFNALVPW
                     LALLAMVVVVASGLTGRFLLADVRERLRERAGELARSGLSPAEVEREVLSVSLLHDTM
                     KQWRKVHLPLTMLFAGLAVVHVAATLLFWRW"
     gene            6459..7202
                     /locus_tag="P5164_07525"
     CDS             6459..7202
                     /locus_tag="P5164_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class III cytochrome C family protein"
                     /protein_id="HRY43781.1"
                     /translation="MRRTWISLVATIAAATAAALLFPYRAITPGSLSAGHLGQRDDCL
                     SCHTLFRGAPAEKCAACHGPGDVGVRTTKGELLARPKERARRIHEALRTGCERCHSEH
                     GSRAGAGRFAHDLLPAEVAAGCAACHHPDRPKDDLHAAAAGECGTCHRVSGWKPASFD
                     HEKSFRFDRHHPPRCADCHAPGRSLKEYSCTGCHEHALARLQAKHLEEGITDLDRCRR
                     CHPSGDEDDTIRGERRDGGRGERRRGEDD"
     gene            7377..8831
                     /locus_tag="P5164_07530"
     CDS             7377..8831
                     /locus_tag="P5164_07530"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRY43782.1"
                     /translation="MSHASRIRAAVAGLLGALLLAPSALGQDAFPGNDEIRRRLDERL
                     ARGGGVGVVVGLLEADGTRRFVAAGSSGNGVPLDEHALFEIGSVTKPFTATLLADMVA
                     RGEVSLDDAVAKYLPAGTRVPERNGRAITLATLATHTAGLPRNPTNLRPADPKDPFGD
                     YGLDRLYEFLATCELPRDPGESWSYSNVGFGLLGHALERRAGTTYAKLLAGRVLAPLG
                     MTETSVGLDPALQRKAVQGYDEAFAPAPMWDLGPLPAAGAIRSTASDMLSFAAANLSA
                     ESDRLHAAMRAAHAPRVPMKDDQRVGLAWMVAKPKTRTITWHWGGTGGFRSYLGLDLE
                     GRRAVVILTNASQMWNEFGHNLLDPEVPLGPASADGLFAKLLREKGLDAALARVRELK
                     AKEPAAWSFEEAHFNAAGYRLLSEKRPADAVKVFTLGTELFPEIGNPWDSLGEALLAA
                     GEKDKAIAAYRRSVELDPKNRNGLEALEKLTKAP"
     gene            complement(8875..9789)
                     /locus_tag="P5164_07535"
     CDS             complement(8875..9789)
                     /locus_tag="P5164_07535"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRY43783.1"
                     /translation="MAAPALDLDLAHLRTFHAVVKAGGFSAAAERLHRTQSAVSHAVN
                     KLERSAKVRLLDRRGRQVVPTPEGKVLHEACERVFATLEEAEEALQRERKRAEGRIRL
                     GATVEFGTSVLMRHIRPFLDANPGLEMDFHLSHDLLTPLLRDDVDLAIDCVAHATPDL
                     ERTPLFREAYMVACAPEYLAAHRIEEPADLARCAVLSIDKEGGWWERFLGTFPKSRRP
                     AFGRVVAINHIRGLITAAVSGLGVLLAPSYSVKSELERGALVPLFPAFRPLEDRFALY
                     QKAGKARLARHRLLTEYLERLTPEEFGA"
     gene            9879..>10309
                     /locus_tag="P5164_07540"
     CDS             9879..>10309
                     /locus_tag="P5164_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRY43784.1"
                     /translation="MHMRVETAKETLHALGIEEKNPGGFGGEWVGSGKVQQVVSPVDG
                     VALASVVNVTKEEFDAVLAKTHAAFATWRTVPAPKRGEVVKAIGEELREKKEQLARLV
                     SLEMGKTLREGLGEVQEMIDICDFAVGLSRQLYGLTMPSERR"
BASE COUNT         1379 a         3802 c         3677 g         1451 t
ORIGIN      
        1 ctcggcgttt ccgaggcggg cggagctttc accattcccc tcgtggggca gggcctgaag
       61 acgggtccga acctgccgct cgacgtcgtc tcgaccgtgt ccgccttcga gctccacggc
      121 gtgggctccg gggtgtatgg ccagatccgc ccctgggccg gagcggcgag ctcgattccc
      181 gcgagcggtt tcgggccgga tgcgaccgtc tggttcggaa tcgggggtac gagcggctgg
      241 ctgagcccgt cggagagcca gtacacgatc gagatcgacc gcaacggcga cctcactccc
      301 gacgccacgg tcacgacgat gccgacgagc gacgtcccga gcccggacga tccgaacgtg
      361 aggtccgccc cgaccgacgt gatcgtcgcc ggcaccagca tcgcgagggg cgcggccgca
      421 aagatctacc gatacgtgaa cctcgatccg gcgttgcgcg acacgaatct cttcggatcg
      481 aacgtcctcg tcgtccccgc ccccgcgggt gagaacggcc tcggcctttc ggcggggaac
      541 gcgaggttcc gctacaggat cacgaggtcg agccggctct tccccgtgac cggcaccgac
      601 gagacggctt ggtacacctt cgacgcggag aagccgggct actcggtcct cgcggcggac
      661 ggcaccccgt tccacgacga cctcggaggc accgctctcc cggcgcggta cgacccggcg
      721 gcggcgcagg cggccggggc gctcggagtg ctgttactcc accaccacaa cccaaagtct
      781 tctcggatcg agaccctgac ggagcgcaac acgccgccga cggtctcgat cgccgcgccg
      841 accccgcaga cggcgttcat ccccgccggg ctcggcctcc acttccacgc ggagggatcc
      901 gaccccgacg ccggtgacat gctctcctat ctgtgggacc tctgcgacgg ccggaagaag
      961 accggtgccc tcgtgacggc ctcgttcccg cgcgcggggt cgtgcacggt gcgcgtgacg
     1021 gtgaccgacg gcgcgggcgc gaccgcgacg gcccaggtcg tcgtcacggt cgaggagccg
     1081 gaaccggacc tcggcctctc caagctcctg ccggtggtcc tcgacgtgca gggcgtcggc
     1141 ggtgcgccgt tcacgacgga gctgacgctc gtctcgcgga tgccggcgct cacgactgtg
     1201 cttctccgtt acaccgcagc cgtgggctcg ggcaccggat gggccggcgt cggccttgag
     1261 gggaggggga cccgcgtcat tccggacgtc atcgcctacc tcaggaggca gggcctcccg
     1321 atccccgacg atgggacgaa ccaggtcggc acgctcgagg tcatgcccct cggagcgacg
     1381 aagccctcgg actacttcgt cggcgcgagg acgtcgacgc cgggagacgg cgggagcttt
     1441 ggcctcttct acgcggacgc ccggaccgcg acggcgaccg cggtcgtcgc cggcctccag
     1501 gagaacgacg cgatgaggtc gaacctcgcg gtcgtgaacg ccggggccga gccggtgacg
     1561 ctcgcgatcc gcctcctcgg cccgcttggc gaggatctcg gagcgctcga ggacgcgacg
     1621 ctccccgcct ggggctggaa gcagttcgac cggcccctgc tcgggaaagc cgcttccgga
     1681 cgagccctcg tggcgcggat cgcggggagc tccccgttct cggcctacgg agtcctgaac
     1741 gacgcgggga cctccgacgg ctcgtacatt ccgccgcacc ttccgggcgc cgccgggccg
     1801 gcggacctga tgatcccggt cgtcctcgac gtccagggcc tcggcacgag ccgctatgcg
     1861 acggaagtga cgctcgcgaa cctcggcacg acgccgctcg cgctcaccct cgcgtacggc
     1921 gctacggcgg agttcggggg cggctcgggc tcggtgccgc tgacgctcgc ggcgggagag
     1981 cagcggatcc tccaggacgt tctctccttc ctgcgcgccg gaggcctcga gattccggga
     2041 gacggggcca acgtcgcggg atcgctcctg gtgaaggcgc cggccggcgc ttcgtccggc
     2101 gctctcgtcg cgggagcgag gattcacacg aggtcgaccc tccggccggg gaccttcggc
     2161 gtctactacc ccggcctgag cgcggacgaa tcggccgacg gcccggtgtc ggtgaacggc
     2221 ctgcggcagg acgcgtcgat gcgctccaac gtcgcgctcg tcaacgtcgg agacgccggc
     2281 gtcgcgacgc tccgaatcac gttcttcagc gagtccgggc agccgctcga gcgccccgag
     2341 gaacggacgc tcaacgccgg tgagtggctg cagctgggtc agcccctcgc cacgcggggc
     2401 gccaccgccg gatctgcgag gatcgagagg atccggggga gatcgcggtt cctcgcctac
     2461 ggcgtcctga acgacgcggc gacctcggac gggagctacc tgccgatgtc acggtgacga
     2521 cccggcgccc cccgcgccag cgcaacgccc cgaactcttc cggcgacagc cgctcgaggt
     2581 gctccctcgt tccgccttcg cgacggtagg cgcgccagag gccgccggcg agatcggcgc
     2641 attcctggtc gagaggatcg acccggaccc cggccgcttc ccgagaattc tccaggcgcc
     2701 attcggcctc ccggaccacc gcgcggcgtg ttcggcagcc gcggaatcgg gccggtctct
     2761 ccgcggtcga agagcgagaa gtcatctcat cgtccggcgg aacgacggcg cgggcgagat
     2821 ccgctgcggg tcgggtgcga tacttctcgc gttgtcgcgc accgtccggt ccgccctgct
     2881 cctcgccgcg gccctcctgc cgctcgcggt ccaggcgggc gccgtgatcg acctcctcga
     2941 cgcgagccgc gaggcccacc cggcgaccct cgtcgagctc ctcgtccacg gccacaccca
     3001 cgggaggaag gtcgtgtccc acgcccacgc ggcgccgggc gaccgcgatc gcgggccgcg
     3061 gctcggcgcg cccgaccagg tggcgacgct cccgacgacg gccggcgcga tgacggccgt
     3121 cgcctcctcg acgcggtacc cccacgagcc ggccgcggtc ggctcgtcgc ttccgccgaa
     3181 gttcctcctc cacgcctcgc tccttcgctg agaccttccc gtcggccggc gcgccgtttc
     3241 cggcgcgcgc gtcacctccg ctgaagaatg gaggatcgcg gtgggagaag agctggaagg
     3301 atccgcaatg gaacggacgc cggagctccc ggcccgggtc gacgtcgtcc ggccttcggg
     3361 cctgaccgag gaggaggccg ccaggcgcct cgcccgggag ggtccgaacg agctccccgc
     3421 ctcgaagaga cgcgggccgt tccggattgc cctcgaggtc gtgaaggagc cgatgttcct
     3481 cctcctgatc gcctgtggct ccctctacct cctcctcggc gacgtcggcg aggccttcct
     3541 cctcctcggc ttcgtcttcg tgatcgtcgg catcaccttc gtccaggagc gcaagacgga
     3601 acgggcgctc gacgcgctcc gcgacctcgc gagcccgcgc gccctcgtcg tgaggaacgg
     3661 cgagaagcgc cggatcgcgg gacgcgacgt cgttcgcggc gacgtcctcc tcctcagcga
     3721 gggagaccgc gtgccggccg acgcggtggt ccgccgcgcc acgagcctgt cggtcgacga
     3781 gtcgctcctc acgggcgagt ccgtgccggt cggcaagcag ccggcagcgg gcgccgcgcc
     3841 cgcggccgcg ccgggcgggg acgacacgcc gttcgtcttc tcggggacgc tcgtcgtgaa
     3901 gggccagggc gtggccgagg tcgtcgccgt cggtgccgcc acggagatgg ggaagatcgg
     3961 gcgggcgctc gcggtgctcg agccggagga cacgccgctc cagcggcaga cggcgcgcct
     4021 cgtccggctc ttcgctctca tcgggttcgg cctctgcgcc acggtcgttg cggcctacgc
     4081 cctcacccgc ggggacttcc tcaaggggct cctcgccggc ctcaccctcg ggatggcgat
     4141 gctgccggag gagttcccgg tcgtcctcac ggtcttcctc gccctcggcg cctggcgcat
     4201 ctcgaggcac cacgtcctca cgcggcgcat ccccgccgtc gagacgctcg gcgcggccac
     4261 cgcgctctgc gtcgacaaga cgggcaccct cacgatgaac cggatgtccg tcgccgagct
     4321 ggcggtcgga gacgaggtcc tcgcggtgga tccgaagggg gcgtcgctcc cggaggactt
     4381 ccacgagctc gtcgagctcg cgatcctggc gagcccggcg gacccgttcg acccgatgga
     4441 gaaggcgatg cgcgagctcg gggagaggac gctcgcgggg accgagcacc tccacggcga
     4501 ctgggagctc ctccgcgagt acccgctctc ccggcagatc ctcgcgatgt cgcgggtctg
     4561 gcgctccccc gacgggagcg agttcgtgat cgcggccaag ggggcgcccg aggcgatcgc
     4621 cgacctctgc catctcgggc cggaagagac cgcgcggctc gagcgccgca tcgcagacct
     4681 cgcctcccgc ggccgccgcg tgatcggcgt cgcgagggcg gcgttccgcg ggcgggccct
     4741 ccccgcgggg cagcacgact tcggtttccg cttcgtgggg ctcctggctc tctccgaccc
     4801 ggtccggccc ggcgtcccgg aggcgatcgc cgagtgccgg cgcgcgggga tccgcgtcct
     4861 gatgatcacc ggcgactacg cggggacggc ccgcagcatc gcgcgggaga tcggcctcgc
     4921 cgacccggag acggtcgtca ccggcccgga gctcgacacg ctcgacgacg cggccctcgc
     4981 cgcgcgggtc cgcgaggcgt gcgtcttcgc gcggatggtc cccgagcaga agctccgtct
     5041 cgtctcggcc ctgaaggcga acggggagat cgtcgccatg acgggggacg gcgtgaacga
     5101 cgcgcctgcg ctgaaagcgg cgcacatcgg gatcgcgatg ggggggcgcg gcaccgacgt
     5161 cgcccgcgag gcggccgcgc tcgtcctgct cgacgacgcc ttcgagtcga tcgtgaaggc
     5221 ggtgcggacc ggacgccgga tcttcgacaa cctccggaag gcgatgctct acatcttcgc
     5281 cgtccacgtc ccgatcgccg ggctgtcgct cttccccgtc ctcctcgggt ggcccctcgc
     5341 gctcctgccg atccacatcg tcttcctcga gctcatcatc gatcccgcct gcaccgtcgt
     5401 cttcgaggcc gaagaggggg aggagggcgt gatgcgccgg cctccccggc cgctcgacga
     5461 gccgatcttc ggcggccgcg gcctcctcct cggcctcgtc caggggctcg gcgagctcgt
     5521 ggtcgacctc gcgctctacg gctgggcgct cgcggcgcac ggggagcccg tcgcccgcgc
     5581 cctcgccttc acgacgatgg tcctcgggaa cgtggggctc atcttcctca accgcgcggg
     5641 ctcccgctcc ctcctcgaga ccctccgcgc gccgaaccgc ccggagcgct gggtcacggg
     5701 aggggccttc gccttcctgg ccctcgccct cggcgtcccc ggagcgcggg agctcttcgg
     5761 attcgcgacg ccgcaccccc agctcctcgc cctttcggcc gccggggcgc tcgtgagcct
     5821 cgccgtggcg aacctcgcgc ggcacctgct cgccgagcgc acggcgacgg ggaggtgagc
     5881 cgtggccctc cgcttcacgc tcacgcgact tctcccgtcc gtcgccctct tcctcgcgct
     5941 gctcggcggc gccctcctcg ccgacctcgt cctccatcgc gccggcctcg ccggggtcgg
     6001 acgctggggc ggcctcgcgg ggacggtcct cctcgccgcg tcgttcctct actccgcgcg
     6061 gaagcggaag ctcctcttcg cggggtcgcc gaaggggctc ctccgcctgc acgagacgct
     6121 cggctgggcc ggggcgctcc tcgtcctcgt ccacggcggg atccacttca acgcgctcgt
     6181 gccgtggctg gcgctcctcg cgatggtcgt cgtcgtcgcg agcggcctca ccggacgctt
     6241 cctcctcgcc gacgtgcgcg agcgcctccg cgagagggcg ggggagctgg cccggtccgg
     6301 gctctccccg gccgaggtcg agcgggaggt cctctccgtc tccctgctcc acgacacgat
     6361 gaagcagtgg aggaaggtcc atctcccgct gacgatgctc ttcgcgggcc tcgccgtcgt
     6421 ccacgtcgcg gcgacgctcc tcttctggag gtggtgacgt gcggcggacc tggatctccc
     6481 tcgtcgccac gatcgcggcc gcgacggcgg cggcgctcct cttcccctac cgggccatca
     6541 ctcccgggtc gctctccgca gggcacctcg gccagcgcga cgactgcctc tcctgtcaca
     6601 cgctcttccg cggcgcgccc gcggagaagt gcgcggcctg ccacgggccc ggcgacgtcg
     6661 gcgtccggac gacgaagggc gagctgctcg cgcggccgaa agagcgtgcg cgccggattc
     6721 acgaggcgct gcgcacgggc tgcgagcgct gccattcgga gcacgggagc cgcgcgggcg
     6781 ccgggcgctt cgcccacgac ctcctgcccg cggaggtggc ggcggggtgc gcggcctgtc
     6841 accaccccga ccgcccgaaa gacgacctcc acgcagcggc ggcgggcgag tgcggcacgt
     6901 gccaccgggt ctcggggtgg aagccggcct ccttcgacca cgagaagtcc ttccgtttcg
     6961 accggcacca cccgccccgc tgcgccgact gccacgcgcc cggccgcagc ctgaaggagt
     7021 actcctgcac cggctgccac gagcacgccc tcgcgcgcct tcaggcgaag cacctcgagg
     7081 aagggatcac ggacctcgac cgctgccgtc gctgccatcc ctcgggagac gaggacgaca
     7141 cgatccgcgg cgagcggcgg gacggcggga ggggagagag gcgccgcggc gaggacgact
     7201 gacggcgcgg cgggccgacc tcggccggtc gacgagcctg tcgggccgga cgacggccgc
     7261 tcgggggcgg agcgcgatgc cccgcgagtc cggcctgcac gaagcccgcg aggagaccga
     7321 gtctcctcga accggcggag cgcgacgtcc gtatcccgag tccgggaggt gtcgacatga
     7381 gccacgcatc gcggatccgg gcggccgtcg cagggttgct gggggcgctc ctcctcgcgc
     7441 cgtcggccct cggccaggac gccttccccg ggaacgacga gatccggcgc cggctcgacg
     7501 agcgcctcgc ccgcggcggg ggcgtgggcg tcgtcgtcgg cctcctcgag gcggacggca
     7561 cgcggcggtt cgtcgccgcg ggctcctcgg ggaacggcgt cccgctcgac gagcacgccc
     7621 tcttcgagat cggctcggtc acgaagccct tcaccgcgac gctcctcgcc gacatggtcg
     7681 cgcgcggcga ggtctccctc gacgacgcgg tcgcgaaata cctcccggcg ggaacgcgcg
     7741 tcccggagcg gaacggcagg gcgatcaccc tggcgacgct cgcgacgcac accgcggggc
     7801 tcccgcggaa tccgacgaac ctgaggcccg ccgacccgaa ggacccgttc ggggactacg
     7861 gcctcgaccg gctctacgag ttcctcgcca cctgcgagct gccgcgcgac ccgggcgaat
     7921 cgtggtccta ctcgaacgtc ggcttcggcc ttctcggcca cgcgctcgag aggcgcgccg
     7981 gcacgacgta cgcgaagctc ctcgccgggc gcgtgctcgc ccctctcggg atgacggaga
     8041 cgtcggtcgg cctcgatccc gcgctccaga ggaaggcggt ccagggctac gacgaggcgt
     8101 tcgcgcccgc gccgatgtgg gacctcggcc cgctccccgc ggccggcgcg atccgttcga
     8161 ccgcgtccga catgctctcc ttcgcggccg cgaacctctc ggccgagagc gatcgcctcc
     8221 acgcggccat gcgggcggcg cacgcgcccc gcgtcccgat gaaggacgac cagcgcgtcg
     8281 ggctcgcgtg gatggtcgcg aagccgaaga cgaggacgat cacgtggcac tggggcggca
     8341 ccggcgggtt ccggagctac ctcgggctcg acctggaggg gcgccgggcc gtcgtgatcc
     8401 tgacgaacgc gtcgcagatg tggaacgagt tcggccacaa cctcctcgac cccgaggtcc
     8461 cgctcggacc cgcttcggcc gacggcctct tcgcgaagct cctccgcgag aagggcctcg
     8521 acgcggcgct cgcgcgcgtg agggagctga aggcgaagga gccggccgcg tggtccttcg
     8581 aggaggccca cttcaacgcg gcggggtacc ggctcctttc cgagaagcgc cccgcggacg
     8641 ccgtgaaggt cttcacgctc ggcaccgagc tcttcccgga gatcggcaac ccgtgggaca
     8701 gcctcggcga ggcgctcctc gccgccggag agaaggacaa ggcgatcgcc gcctaccgcc
     8761 gctccgtgga gctcgacccg aagaaccgga acgggctcga ggcgctcgag aagctgacga
     8821 aggcgccgtg acccggcggg gcggccgccg cctccttcgg cttccgctcc tgccctacgc
     8881 cccgaactcc tccggcgtca gccgctccag gtactccgtc aggaggcggt gcctggcgag
     8941 gcgcgccttg ccggccttct ggtagagggc gaagcggtcc tcgaggggac ggaacgccgg
     9001 gaagaggggg acgagggcgc cgcgctcgag ctcgctcttc acgctgtagc tcggggcgag
     9061 aaggacgccg aggccgctca cggcggcggt gatgaggccg cggatgtggt tgatggcgac
     9121 gacgcggccg aacgccgggc ggcggctctt cgggaacgtc ccgaggaagc gctcccacca
     9181 gcctccctcc ttgtcgatcg agaggacggc gcagcgggcg aggtccgccg gctcctcgat
     9241 ccggtgagca gcgaggtact cgggcgcgca ggcgaccatg taggcctcgc ggaagagcgg
     9301 cgtcctctcg aggtcgggcg ttgcgtgcgc gacgcagtcg atcgcgaggt cgacgtcgtc
     9361 gcgcaggagc ggcgtcagga ggtcgtgcga gaggtggaag tccatctcca gccccgggtt
     9421 cgcgtcgagg aacgggcgga tgtggcgcat caggacgctc gtcccgaact ccaccgtcgc
     9481 gccgagccgg atccgcccct ccgcccgctt ccgctcgcgc tggagcgcct cctcggcctc
     9541 ctcgagcgtc gcgaagaccc gctcgcacgc ctcgtggagg accttgccct cgggggtcgg
     9601 gacgacctgg cggccccgcc tgtcgaggag gcggaccttc gcggatcgct cgagcttgtt
     9661 cacggcgtgg ctgacggcgc tctgggtccg gtggaggcgc tcggcggcgg ccgagaagcc
     9721 tccggccttc acgacggcgt gaaaggtgcg gaggtgggcg aggtcgaggt cgagggcggg
     9781 cgcagccatg gatgaatgat atcgattgaa agaatgaaaa caatgagctt cataaatagg
     9841 agattctgtc gcataacctc tctacggagg gactggtcat gcacatgcgg gtcgagacgg
     9901 cgaaagagac gcttcacgcg ctcgggatcg aggagaagaa ccccggcgga ttcggcggcg
     9961 agtgggtcgg gagcgggaag gtccagcagg tcgtttcccc ggtggacggc gtggccctcg
    10021 cctcggtcgt caacgtgacg aaggaggagt tcgacgcggt cctcgcgaag acgcacgcgg
    10081 ccttcgccac ctggcgcacc gtcccggcgc cgaagcgggg cgaggtcgtc aaggcgatcg
    10141 gcgaggagct ccgcgagaag aaggagcagc tcgcgcggct cgtctccctc gagatgggga
    10201 agaccctccg cgaggggctc ggcgaggtgc aggagatgat cgacatctgc gacttcgccg
    10261 tcggcctctc ccgccagctc tacggcctca cgatgccgag cgagcggcg
//
LOCUS       DAOTEZ010000109        10266 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4586_length_10266_cov_6.823328, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000109 DAOTEZ010000000
VERSION     DAOTEZ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10266
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4586_length_10266_cov_6.823328"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="P5164_07545"
     CDS             <1..353
                     /locus_tag="P5164_07545"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRY43785.1"
                     /translation="EKGENPAVNALVERYVEPGVEIPWLSNARQPDNAYFPHAAHVTG
                     EKVACARCHGPHGESTAVRPVQVNRLSGYPRDIWGPSISRLRREEWQGMKMSDCIACH
                     RAGGRESACIDCHK"
     gene            364..2229
                     /locus_tag="P5164_07550"
     CDS             364..2229
                     /locus_tag="P5164_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRY43786.1"
                     /translation="MKLEVSRRDLLLAGTGAAAGIALSPVPWKALDDLSIWTQRPPTP
                     TPRPAGPLAFRFTSCTLCPAACGLKARSFGPQPVGFSGVPGHPASDGGLCPLGLTAHH
                     LAKHPLRAVAPERVSGGKRETIDRAAASAALSAAIAAAAKDPKRSLLVVDGRPGRSLS
                     RTFRALAAATNGRYAVPGSPSPALDTLAARFDGPLSLGVDLSKARRVVSFGTPVLTDA
                     GTPGLLARLRAEGRRKPKGERLEVVHVGATRTKSAGFADRFVPIAPGTEAALALGLAH
                     VLLDEKLADAAFLAARTTGLAGLEALAARFAPPLVEQKTGVPAGDVVALARDLAASRP
                     AVVLGGDPAEPLSAEADAAIAALNLLLGAPSGPLVARRAAPSEPDGDAKLAAVTALDA
                     VPERSVSLLLLDGTVPEGLVPWARLEKLLADGAVVASFSAFRAGLGEKATLLVPGPAP
                     LEEAVELDGPADAPLATFALAPALLAPPKELVLPEELLREAASAAGFELPAPPAGRAA
                     AIVAAKRGQLFDPSTATTTPVSSLADAAALETAFAAGACWVDDPAPAPAGRFALLSDG
                     EAPRLLESALGAPGPEPARVAALPRTALSAAPSSPLLTKLTRETALFPAPSAAKA"
     gene            2232..2990
                     /locus_tag="P5164_07555"
     CDS             2232..2990
                     /locus_tag="P5164_07555"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRY43787.1"
                     /translation="MTATQQTSTLPTHRYTLVVDLDRCNGCGSCATACAVENNIPPAP
                     ERSNVRTGVVWLRVHDVATVDPSGRSTFVPIPCQQCDEPPCVDVCPQRAVEYDPRTGI
                     VGQIPVRCLGCRYCMVACPYHTRSFNWWSPEWPEGMKASLNPDVSIRQRGVVEKCNFC
                     VQRLQAARAKASAAGKTDIDPADYVPACVEACPSRALSFGDANDADGEVAKLAKDPES
                     FRLLAALGTSPKVLYRSSDPVVRERLSRTGVTRG"
     gene            2983..4218
                     /gene="nrfD"
                     /locus_tag="P5164_07560"
     CDS             2983..4218
                     /gene="nrfD"
                     /locus_tag="P5164_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HRY43788.1"
                     /translation="MDDTLIARGLKRTPLPRFLLWLLPWVGVLAFGLYAVYLCFALGL
                     NQTNMDNRFAFGLWIYLDLTVIALGAGAFFTGFLLYILKRKELKAVINSAVVIGFICY
                     SGAVGILMVDVGQPLRAWFTFWHPNVHSMLTEVTFCITIYLCVLAIEYLPLVLKNRRL
                     SEVPGLLVLEHQLHKLMVVFAGVGTLLSFFHQGSLGGLYGVLRGNPFAYREHIFIWPT
                     TFFLFVLSAAAVGPSFILLTTWLVGKITRKQLVAPEVLQTLGKISGWLLLVYVVLKGI
                     DSLVWLNSTSPQTGVQVSSFYANPPFGVQVLFAEVVLLGLVPAVLLLSRRVRARTGLL
                     VAVAATACAGVVLNRYVMTIQTLALPSLPFDSFASYTPSWQEVAAFLAVLGYGVLLYS
                     ISFRWLNLFPQERELAAKG"
     gene            4232..5041
                     /locus_tag="P5164_07565"
     CDS             4232..5041
                     /locus_tag="P5164_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43789.1"
                     /translation="MLPGNTDFIADPFNIAFLGLFALILTAVAVTLVRIGLRTRETIA
                     RGKAASMLWHEEFEELPASARGCRHALTGELPGRTCARAFDCRGCETHAKLVAEGKVL
                     PPAEAAGFDVPTDRLYHRGHAWLKPLEDGTVAIGLDDLATRLVGKPDAVVLPAVGSTL
                     AENGPALRLRAGKARVRVASPVTGEVVEHGSLSSDFLLRVKPEGQLDTRALLTPREAA
                     AYFSRELDRLQLALSGSVPALADGGLPVGDLSKAIPEDDRDRVLGEMLLEA"
     gene            5580..5816
                     /locus_tag="P5164_07570"
     CDS             5580..5816
                     /locus_tag="P5164_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43790.1"
                     /translation="MAHPPATAASSWLKQWRAAGPALEEVRRRELRELRDDEALAAAE
                     ALLSLAASSPLPEERRVSSGLVAQQALLHRRRPR"
     gene            5813..6391
                     /locus_tag="P5164_07575"
     CDS             5813..6391
                     /locus_tag="P5164_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43791.1"
                     /translation="MNPIFAAAREIQDFCRGRGWRSCVIGGLAVQRWGEPRLTRDVDV
                     TLLTGFGAEASYVDGLLSAFRGRLSDAREFALRNRVLLLSSSTGTPIDVALGAMPFEE
                     AAVSRATPFPIGPDFEVLTCGAEDLVVFKAFAGRPRDWLDIEGIVARQATRLDVPLIW
                     RELLPLLELKDDSETEGRLRRLLASSGAEDVE"
     gene            complement(6431..8947)
                     /locus_tag="P5164_07580"
     CDS             complement(6431..8947)
                     /locus_tag="P5164_07580"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HRY43792.1"
                     /translation="MTEQRVPGPGDEAESVPRARVEAWKRAIHDSLVTAVGRFPAMAT
                     RNDRYLAVAHAVRDRLVRRWMRTGEHYYRAKVRSVCYLSAEFLLGPHLGNALVALGVL
                     DEVRQAVEEMGWGFDEVLAQEEEPGLGNGGLGRLAACFMDSLATLQIPAIGYGIRYEF
                     GIFDQEIRDGWQVEKTDKWLQYGNPWEVPRPEIAFEVKLGGRTEAFTDEAGRYRVRWV
                     PAQVVKGVAYDTPIPGYRVGNVNLLRLFKAEAAESFDFAAFNVGDYYGAVEEKVGSET
                     ISKVLYPNDEPAAGKALRLSQQHFFVSCSLQDMIRIHLQASPDLADLADRWAVQLNDT
                     HPAIAVAELMRLLVDEHGMGWDEAWTITRATFAYTNHTLLPEALEKWPVELFGKTLPR
                     HLEIVYEVNRRFLDELRSRFPGDEGLAARLSLVDESGPRSIRMAHLAAVGSHHVNGVA
                     ALHSELLRRDVMRDFAAIWPERFTNVTNGVTPRRFVALANPGLTRLVTAAIGDGWVRD
                     LDELRRLEPLADDAGFREEWRRVKAANRSALAEAARQRAGVSADPASLWDVQVKRIHE
                     YKRQHLNLLHVLTLYRRLKRNPGLDVPPRTVLFAGKAAPGYATAKLIIKAIHSVAEVV
                     NGDPATKGRLAVVFWPDYNVKSSLPIFAGADLSEQISTAGKEASGTGNMKLALNGALT
                     IGTLDGANVEIREAVGAEHFFLFGLTVEEVERTWRDGYRPGERYEADPELREAVDALV
                     SGELTRGDRGLLRPLVHGLLTKDPFLVLADYRAYVDAQGEVEAAWRDPERWTRDSILN
                     TARMGRFSSDRSIRDYAREIWRAVPAPVPVVEGEERPPCG"
     gene            complement(9058..>10266)
                     /locus_tag="P5164_07585"
     CDS             complement(9058..>10266)
                     /locus_tag="P5164_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRY43793.1"
                     /translation="IYFVDLKMPGGIDGIETMMEVKKLRPDASIVIITAYATVDTAIT
                     AMKEGALEYIVKPCNPQEISLLVTRIIKVRNLERENEILRRSLTGRWRFHDLLSKNPR
                     MQEIFGLVKEVASLRSTVLITGESGTGKELVARALHFSGDRAEGPFVGVSCAALTETL
                     LESELFGHEKGAFTGAVERRKGKFELAEGGTIFLDEIGDISAKVQVDLLRALQERRFF
                     RVGGSAEVAVDVRVVAATNADLAEEVRQGRFRDDLFYRLNVVAIHLPPLRERPEDVPL
                     LARSFLERLSHELGKEAEDLSEGALKALLAHRWPGNVRELENAVERALVTAKGRVLTE
                     DDFAFLRPVSEAAPGWSVPTHLPLSDVEKAVIAATLKRNEGNVKETAAVLGIDRSTLY
                     DKLKRYGIER"
BASE COUNT         1290 a         4014 c         3425 g         1537 t
ORIGIN      
        1 ccgagaaggg cgagaacccg gcggtcaacg ccctcgtcga gcgctacgtc gagcctgggg
       61 tcgagatccc gtggctctcg aacgcccgcc agcccgacaa cgcgtacttc ccgcatgccg
      121 cgcacgtgac cggcgagaag gtcgcctgcg cgcgctgcca cggcccgcac ggggagtcga
      181 ccgccgtgcg ccccgtccag gtgaaccgcc tctccggcta cccgcgcgac atctggggcc
      241 cctcgatctc gcgcctccgg cgcgaggagt ggcaggggat gaagatgtcc gactgcatcg
      301 cgtgccaccg cgcgggcggg cgcgagagcg cctgcatcga ctgccacaag tgaagaggac
      361 ggcatgaagc tcgaagtctc ccgccgcgac ctcctcctcg ccggaaccgg cgccgccgcc
      421 gggatcgccc tgtcgccggt cccctggaag gccctcgacg acctctccat ctggacgcag
      481 cgtcccccga cgccgacgcc gcgcccggcc gggccgctcg cgttccgctt cacgtcgtgc
      541 acgctctgcc ccgccgcctg cgggctgaag gcgcgctcgt tcgggccgca gcccgtcggc
      601 ttctccggcg tccccggaca ccccgcgagc gacggcggcc tctgcccgct cgggctgacc
      661 gcccaccacc tcgcgaagca cccgctccgc gccgtcgccc ccgagcgcgt ctcgggcggg
      721 aagcgcgaga cgatcgaccg cgccgccgct tccgccgccc tctccgccgc gatcgccgcc
      781 gccgcgaagg acccgaaacg ctccctcctc gtcgtcgacg gccgtcccgg ccgaagcctt
      841 tcgcgcacat ttcgggctct cgccgcggcg acgaacgggc gctacgccgt tcccggatct
      901 ccctcgccgg ccctcgacac cctcgcggcc cgtttcgacg gccctctctc gctcggcgtc
      961 gacctctcga aggcccgccg cgtcgtctcc ttcgggacgc cggtcctgac ggacgccggg
     1021 acgcccggcc tcctcgcgcg cctccgcgcc gaggggcgcc ggaagccgaa aggcgagcgg
     1081 ctcgaggtcg tccacgtcgg ggcgacccgg acgaagagcg ccggcttcgc cgaccgcttc
     1141 gtcccgatcg cccccggtac cgaagccgct ctcgccctcg gcctcgccca cgtcctcctc
     1201 gacgagaagc tcgccgacgc ggcgttcctc gccgcgcgga cgacgggcct cgccgggctc
     1261 gaggcgctcg cggcccgctt cgccccgccc ctcgtcgagc agaagacggg cgtccccgcg
     1321 ggcgacgtcg tcgcgctcgc gcgtgacctc gcggcgagcc gcccggccgt cgtcctcgga
     1381 ggcgacccgg ccgagccgct ctccgccgag gccgacgccg cgatcgccgc cctgaacctc
     1441 ctcctgggcg cgccgtccgg cccgctcgtc gcgcgccgcg cggcgccttc cgagccggac
     1501 ggcgacgcga agctcgccgc ggtcaccgct ctcgacgcgg tgcccgagcg ctccgtcagc
     1561 ctcctcctcc tcgacgggac ggtccccgag gggctcgtcc cctgggcgcg actcgagaag
     1621 ctcctcgccg acggcgccgt cgtggcgagc ttctccgcgt tccgcgccgg cctcggcgag
     1681 aaggcgacgc tcctcgtacc cggtcccgcg ccgctggaag aggcggtcga gctggacggc
     1741 ccggccgacg cgccgctcgc gacgttcgcc ctcgcccccg ctctcctcgc cccgccgaag
     1801 gagctcgtcc tccccgagga gctcctccgc gaggcggcct ccgccgcggg tttcgagctc
     1861 ccggcgccgc cggccggccg cgccgcggcg atcgtggcgg cgaagcgcgg gcagctcttc
     1921 gacccctcca cggcgacgac gaccccggtc tcctcgctcg ccgacgccgc ggccctcgag
     1981 acggccttcg cggccggcgc ctgctgggtc gacgacccgg cccccgcccc cgccggacgt
     2041 ttcgcgctcc tctccgacgg cgaggcgccg cgcctcctcg agtcggccct cggcgcgccc
     2101 ggccccgagc cggcccgcgt cgcggccctc ccgcgcacgg ccctctccgc cgccccctcc
     2161 tccccgctcc tcacgaagct gacccgcgag acggcgctct tccccgcgcc gtccgccgcg
     2221 aaggcgtgac catgaccgcc acccagcaga cctccaccct cccgacccac cgctacacgc
     2281 tcgtcgtcga cctcgaccgc tgcaacggct gcggctcctg cgcgaccgcc tgtgccgtcg
     2341 agaacaacat cccgcccgct cccgagcgct cgaacgtgag gaccggcgtc gtctggctgc
     2401 gcgtccacga cgtcgccacc gtcgatccct cggggcgctc gacgttcgtc ccgattccct
     2461 gccagcagtg cgacgagccc ccctgcgtcg acgtctgccc ccagcgcgcg gtcgagtacg
     2521 acccgcgcac cggcatcgtc ggccagatcc ccgtccgctg tctcggctgc cgctactgca
     2581 tggtcgcctg cccctaccac acgcgctcgt tcaactggtg gagcccggag tggcccgagg
     2641 ggatgaaggc ctccctcaac cccgacgtct cgatccgcca gcgcggcgtc gtcgagaagt
     2701 gcaacttctg cgtccagcgc ctccaggcgg cccgcgcgaa ggcctccgcc gccggcaaga
     2761 ccgacatcga cccggccgac tacgtccccg cctgcgtcga ggcgtgcccg tcgcgggcgc
     2821 tctccttcgg cgacgcgaac gacgcggacg gcgaggtcgc gaagctcgcg aaggacccgg
     2881 agagcttccg cctcctcgcc gccctcggga cctctccgaa ggtcctctac cgctcgtcgg
     2941 accccgtcgt ccgcgagcgc ctctcccgca cgggggtgac gcgtggatga caccctgatc
     3001 gcccgcggcc tgaagcggac cccgctgccg cgattcctcc tctggctcct cccgtgggtc
     3061 ggcgtgctcg cgttcggcct ctacgccgtc tacctctgct tcgccctcgg cctgaaccag
     3121 acgaacatgg acaaccggtt cgcgttcggc ctctggatct acctcgacct gacggtcatc
     3181 gccctcggcg ccggcgcctt cttcacgggc ttcctcctct acatcctgaa gaggaaggag
     3241 ctgaaggcgg tcatcaacag cgccgtcgtc atcggcttca tctgctacag cggcgccgtc
     3301 ggcatcctga tggtcgacgt cggccagccg ctccgcgcct ggttcacgtt ctggcacccg
     3361 aacgtccact cgatgctcac cgaggtgacg ttctgcatca cgatctacct ctgcgtcctg
     3421 gcgatcgagt acctgccgct cgtcctcaag aaccggcggc tcagcgaggt ccccggcctc
     3481 ctcgtcctcg agcaccagct ccacaagctg atggtcgtct tcgccggagt cgggacgctc
     3541 ctctccttct tccaccaggg ctcgctcggc gggctctacg gcgtcctgcg cgggaacccc
     3601 ttcgcctacc gcgagcacat cttcatctgg ccgacgacgt tcttcctctt cgtcctctcg
     3661 gccgccgccg tcggcccgag cttcatcctg ctcacgacct ggctcgtcgg gaagatcacc
     3721 cgcaagcagc tcgtcgcccc ggaggtcctc cagacgctcg ggaagatctc ggggtggctc
     3781 ctcctcgtct acgtcgtcct gaaggggatc gactcgctcg tctggctgaa ctcgacgagc
     3841 ccgcagacgg gcgtgcaggt ctcgtccttc tacgcgaacc cgccgttcgg cgtccaggtc
     3901 ctcttcgccg aggtcgtcct cctgggcctc gtcccggccg tcctcctcct ctcgcggcgc
     3961 gtccgcgcgc ggaccggcct cctcgtcgcg gtcgccgcga ccgcctgcgc cggcgtcgtc
     4021 ctgaaccgct acgtcatgac gatccagacg ctcgcgctcc cctcgctccc gttcgacagc
     4081 ttcgcgtcct acacgccgtc ctggcaggag gtcgcggcct tcctcgccgt cctcggctac
     4141 ggcgtccttc tctactcgat ctccttccgc tggctgaacc tcttccccca ggagagggag
     4201 ctggcggcga agggctgacg gaggttccga gatgcttccc ggcaacaccg acttcatcgc
     4261 cgaccccttc aacatcgcct tcctcggcct cttcgcgctc atcctcaccg ccgtcgccgt
     4321 gacgctcgtg cggatcggcc tccggacccg cgagacgatc gcgcgcggca aggccgcctc
     4381 gatgctctgg cacgaggagt tcgaggagct ccccgcgtcg gcccgcggct gccgccacgc
     4441 gctgacgggc gagctccccg gccgcacctg cgcccgcgcc ttcgattgca ggggatgcga
     4501 gacccacgcg aagctcgtcg cggaggggaa ggtgctcccg cccgccgagg ccgccggctt
     4561 cgacgtgccg accgaccgcc tctaccaccg cggccacgcc tggctgaagc cgctcgagga
     4621 cggcaccgtc gcgatcggcc tcgacgacct cgccacgcgc ctcgtcggaa agcccgacgc
     4681 ggtcgtcctc ccggccgtcg gttcgacact cgccgagaac gggccggccc tgcgcctgcg
     4741 cgccggaaag gcccgcgtcc gcgtcgcctc ccccgtcacc ggcgaggtcg tcgagcacgg
     4801 ctcgctctcc tcggacttcc tcctccgcgt gaagcccgaa ggccagctcg acacgcgcgc
     4861 cctcctgacc ccgcgcgagg ccgccgccta cttctcccgc gagctcgacc gtctccagct
     4921 cgccctctcc ggcagcgtcc ccgccctcgc cgacggcggc ctcccggtcg gcgacctctc
     4981 gaaggcgatc cccgaggacg accgcgaccg cgtcctcggc gagatgctcc tggaagcctg
     5041 aacctcctcg tcggcgactc gggggcgtcc cccccttccg cccccggcac cgccgaagtc
     5101 cccggggccc gcccggttcc acgcacagga ccgggcgggc gttcttcttc ccactcactc
     5161 ctcgctctct tctcaagtcg ctccaccctc ggcgttccca ccccgcaccc cctcaaagac
     5221 aagcccgcgc gaagcgcgca ccgcaaaggg tggtgggtgg ggggcgcggg gtgggtacat
     5281 agggcttccg cccccggcac cgccgatgcc cccggggccc gaccggtccc acgcacagga
     5341 ccgggcgggc gtttctcttc ccctctctcc tctctccctt ccacccgcga cactccccac
     5401 cccgcacccc atcaaacacg agcccgcgcg aagcgcgcat cgagaagggc ggtgggtggg
     5461 gggcgcgggg tgggtacata ggggggcgcg gggtgggtcc atcgggtagg cggggggcgc
     5521 gctccgatcc gcaggcgagg cgcggggtgc gtccgcaggc ccccccctat actcggccca
     5581 tggcccatcc ccccgccacc gcagcttctt cctggctgaa gcagtggcgg gcggccggac
     5641 ccgctctgga ggaggtgcgg aggcgcgagc tccgcgagct ccgggacgac gaggcgctcg
     5701 ccgcggccga ggcgctcctc tccctggccg cctcgtcccc cctgccggag gagcgccggg
     5761 tctcctcggg tctcgtcgcc cagcaggcgc tgcttcaccg acggcgcccg cgatgaaccc
     5821 catcttcgcg gccgcccgcg agatccagga cttctgccgg ggccggggct ggcgctcctg
     5881 cgtcatcggc gggctcgccg tccagcgctg gggcgagccg aggctcaccc gggacgtgga
     5941 cgtgacgctc ctgaccggct tcggcgccga ggcctcctac gtcgacggcc tcctctcggc
     6001 gttccgcggc cgcctctccg acgcgcgcga gttcgccttg cgaaaccgag tccttctgct
     6061 ctcgagcagc accggcaccc cgatcgacgt cgcgctcggc gccatgccgt tcgaggaggc
     6121 ggccgtctcc cgggccacgc ccttcccgat cggccccgac ttcgaggtcc tcacctgcgg
     6181 cgcggaggac ctcgtcgtct tcaaggcctt cgcaggtcgg ccccgggact ggctcgacat
     6241 cgaagggatc gttgcccgac aggcgacgcg gctcgacgtc cccctgatct ggcgtgagct
     6301 cctccccctc ctcgagctca aggacgacag cgaaaccgag ggccggctcc gccggcttct
     6361 cgcgtcctcc ggagccgaag acgtcgagta gcccgcgcga cgcgggtcgc gccgcgcccg
     6421 cccgccgcgc tcagccgcag ggcggccgtt cctccccctc gacgaccggg acgggcgcgg
     6481 gtacggccct ccagatctcg cgcgcgtagt cgcggatcga gcgatcggaa gagaagcgcc
     6541 ccatacgggc cgtgttcagg atcgagtcgc gcgtccagcg ctccgggtcg cgccaggccg
     6601 cctcgacctc gccctgcgcg tcgacgtagg cgcggtagtc cgcgaggacg aggaacgggt
     6661 ctttcgtgag gaggccgtgg acgagcgggc ggaggagccc gcggtcgccc cgggtgagct
     6721 cgcccgagac gagcgcgtcg acggcctcgc ggagctcggg gtcggcctcg tagcgctcgc
     6781 cggggcggta gccgtcgcgc caggtccgct cgacctcctc gaccgtcagg ccgaagagga
     6841 agaagtgctc ggcgccgacg gcctcgcgga tctcgacgtt cgcgccgtcg agcgtcccga
     6901 tcgtgagcgc gccgttcagg gcgagcttca tgttccccgt cccggaggcc tctttgccgg
     6961 cggtcgagat ctgctcggag aggtcggcgc cggcgaagat cgggaggctg ctcttgacgt
     7021 tgtagtcggg ccagaagacg acggcgaggc gccccttcgt cgccgggtct ccgttgacga
     7081 cctcggcgac ggagtggatc gccttgatga tcagcttcgc cgtcgcgtag ccgggcgccg
     7141 cctttcccgc gaagaggacg gtccggggcg ggacgtcgag ccccgggttc cgcttcagcc
     7201 gccggtagag cgtcaggacg tggaggaggt tcaggtgctg gcgcttgtac tcgtggatcc
     7261 gcttcacctg gacgtcccag aggctcgccg ggtcggcgga gacgccggcc ctctgccgcg
     7321 cggcctcggc gagggcagac cggttcgccg ccttcacgcg ccgccactct tcgcggaagc
     7381 cggcgtcgtc ggcgagcggc tcgaggcggc ggagctcgtc gaggtcgcgg acccacccgt
     7441 cgccgatggc cgcggtgacg aggcgcgtca ggccggggtt cgcgagggcg acgaagcgcc
     7501 gcggggtgac gccgttcgtg acgttcgtga acctctccgg ccagatcgcc gcgaagtcgc
     7561 gcatcacgtc gcgccggagg agctcggagt ggagggcggc gacgccgttc acgtggtggc
     7621 tcccgacggc cgcgaggtgc gccatccgga tcgacctcgg cccgctctcg tcgacgaggg
     7681 agagccgcgc ggcgagcccc tcgtcgcccg ggaaccggct gcgcagctcg tcgaggaagc
     7741 ggcggttgac ctcgtagacg atctcgaggt ggcgcgggag cgtctttccg aacagctcca
     7801 cgggccactt ctcgagcgcc tccggcagga gcgtgtggtt cgtgtaggcg aaggtcgcgc
     7861 gggtgatcgt ccacgcctcg tcccacccca ttccgtgctc gtcgacgagg aggcgcatca
     7921 gctcggcgac ggcgatcgcc gggtgcgtgt cgttcagctg gacggcccag cggtccgcga
     7981 ggtccgccag gtcgggcgag gcctgcaggt ggatccggat catgtcctgc agcgagcagg
     8041 agacgaagaa gtgctgctgc gagaggcgga gcgccttccc ggcggccggc tcgtcgttcg
     8101 ggtagaggac cttcgagatc gtctcggagc cgactttctc ctcgacggcg ccgtagtagt
     8161 cgccgacgtt gaacgccgcg aagtcgaacg actcggccgc ctcggccttg aagaggcgga
     8221 ggaggttcac gttgccgacc cggtagcccg ggatcggcgt gtcgtaggcg acgcccttca
     8281 cgacctgcgc cgggacccac cgcacgcggt aacgaccggc ttcgtcggtg aaggcctccg
     8341 tccgcccgcc gagcttcacc tcgaacgcga tctcggggcg cgggacctcc cacgggttgc
     8401 cgtactggag ccacttgtcg gtcttctcga cctgccagcc gtcccggatc tcctggtcga
     8461 agatgccgaa ctcgtaccgg atcccgtagc cgatcgccgg gatctggagc gtcgccagcg
     8521 agtccatgaa gcaggccgcg aggcgcccga ggccgccgtt gccgagcccc ggctcctcct
     8581 cctgcgcgag gacctcgtcg aagccccagc ccatctcctc cacggcctgc cggacctcgt
     8641 cgaggacgcc gagggcgacg agggcgttgc cgaggtgcgg gccgaggagg aactcggcgg
     8701 agaggtagca gacggagcga accttcgccc ggtagtagtg ctcgcccgtc cgcatccagc
     8761 gccgcacgag acggtcgcgg acggcgtggg cgacggcgag gtaacggtcg ttccgcgtcg
     8821 ccatggccgg gaagcgcccg acggccgtga cgaggctgtc gtggatcgcc cgcttccacg
     8881 cctcgacccg tgcgcggggg acgctctccg cctcgtcgcc gggtccgggg accctctgct
     8941 ccgtcatggc tcgccctccg tcgcccggcc gcgcgccgcg aaagcggcag gcggccggcg
     9001 cagctcgatt cggacgtatt gcccgaatgc tagcgcagga gacgaagcgc cggcacccta
     9061 ccgctcgatt ccatacctct tcagcttgtc gtagagcgtc gagcggtcga tgccgaggac
     9121 ggcggcggtc tccttcacgt tcccctcgtt ccgcttcagc gtcgcggcga tgacggcctt
     9181 ctcgacgtcc gagaggggca ggtgcgtcgg gacgctccag ccgggcgcgg cctcggagac
     9241 gggacggagg aacgcgaagt cgtcctcggt caggacgcgc cccttcgcag tgacgagcgc
     9301 acgctcgacg gcgttctcga gctcgcggac gttccccggc cagcggtggg cgaggagcgc
     9361 cttgagcgcc ccctcggaga ggtcctccgc ctccttgccg agctcgtgcg agaggcgctc
     9421 gaggaaggag cgggcgagga gcgggacgtc ctcgggcctc tcgcggagcg gcgggaggtg
     9481 gatcgcgacg acgttgaggc gatagaagag gtcgtcgcgg aagcgcccct gccggacctc
     9541 ctcggcgagg tcggcgttcg tggcggcgac gacccgtacg tcgaccgcca cctccgcgct
     9601 cccgccgacg cggaagaagc gccgctcctg gagggcgcgg agcaggtcga cctggacctt
     9661 cgccgagatg tcgccgatct cgtcgaggaa gatcgtcccc ccttcggcga gctcgaactt
     9721 ccccttccgc ctctcgacgg cgcccgtgaa ggcccccttt tcgtggccga agagctccga
     9781 ctcgaggagc gtctcggtga gggcggcgca ggagacgccg acgaaggggc cctcggcacg
     9841 atcgcccgag aagtggaggg cccgcgcgac gagctccttg ccggtgccgc tctcccccgt
     9901 gatcaggacg gtgctgcgga ggctcgcgac ctccttcacg agcccgaaga tctcctgcat
     9961 ccgggggttc ttcgagagga ggtcgtggaa gcgccagcgc cccgtgaggc tccgccggag
    10021 gatctcgttc tcccgctcga ggttcctcac cttgatgatg cgggtgacga ggagcgagat
    10081 ctcctgcggg ttgcacggct tgacgatgta ctcgagggct ccctccttca tcgccgtgat
    10141 cgcggtgtcg accgtcgcgt aggccgtgat gatgacgatc gaggcgtcgg gacggagctt
    10201 cttgacctcc atcatcgtct cgatgccgtc gatccccccc ggcatcttca ggtcgacgaa
    10261 gtagat
//
LOCUS       DAOTEZ010000110        10204 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4623_length_10204_cov_6.655828, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000110 DAOTEZ010000000
VERSION     DAOTEZ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10204
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4623_length_10204_cov_6.655828"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="P5164_07590"
     CDS             complement(<1..1059)
                     /locus_tag="P5164_07590"
                     /inference="COORDINATES: protein motif:HMM:NF019605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY43794.1"
                     /translation="MTNAAPRRRRAAPAAASNAAPPAARRPARPKRPAAAPKPIVDGT
                     PHVHPEGEFLWESSPSRYIARMSATTGREVLRPVDPEELLITAHRDKNPFEVTTSFLA
                     SRLVQVTGNEEKVREAMKSANAFGLRVAGRLGRLTGSHAVERGAKKLIGVVDRSVFFQ
                     FREPTHRTARDVSQRIERLRADARAALAARGRSPRLRVLLTGATGFLGQEILAQAASD
                     RRIAEVVSVIRPQTVRDPKTREVIRVLSPTERGRLLLSRLGVGGRAARKFRFVEGDIE
                     KPGLGIPEDELATLERTVTHVVHCAASVSFDDTYESSFRANVLGCKNALDFSLRLQRA
                     KGSRFVLHVAIETSYIHGR"
     gene            complement(1118..3592)
                     /locus_tag="P5164_07595"
     CDS             complement(1118..3592)
                     /locus_tag="P5164_07595"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012750014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HRY43795.1"
                     /translation="MSVAPPLPRTGEELREAIRWHVRHSLGKAWEDATNGDLFQALGL
                     AVRDRAWERLLATEKRYRQAGAKRVYYLSMEFLIGRSLVDSLHNLGLHDAAVQALAEL
                     GHGLDQVREEEVDAGLGNGGLGRLAACFLDSLATLHLPGYGYGLFYQYGLFRQEIDNG
                     YQKERPDRWLHAAMPWTVERPEEAVLVPVYGRIVDTVDRKSQYNPMWMDWKILVGVPH
                     EMPVVGYGGETVNALRLYSARASDEFNMEFFNSGDYVQAVQESIGSETISKVLYPSDR
                     VQKGKELRLLQEYFFVACALRDILRRFLREGHPIAAFPDRIAIQLNDTHPALAVAELM
                     RLFVDEHDVAWERAWEMTVATLGYTNHTLLPEALEKWPLPLVETVLPRHLQIIYEINR
                     RFLDEVAHRAPGDQEVLARVSMIEEGEPKQVRMAQLAAAGSHSINGVAALHSRLITEN
                     LFPDLYRLHPERFNNKTNGVTPRRWLLKSNPSLAALLTETAGPGWVTDYGLLREPLDR
                     LAEDAAFRDRFRAVKRENKVRLARIVADLTRVRVDPDSLFDIQIKRIHLYKRQLLNAL
                     HVVHLYFRIVEDGEVPKAPRTFVFAGKAAPGYWIAKQVIKLIHAVGERVNRDTRVKGM
                     LKCVFLPDYRVSLAERIIPAADLSEQISTAGMEASGTGNMKLSMNGALTIGTLDGANI
                     EIMEEAGEENIFIFGHTAEELDRLKQPGAYSARRVYEDDPRVRRVLDAFRTPLFAPFE
                     PGIFSWLFENVVENHDPYFHLADFDAYATAQERVADQWTDAAGWTRKAVLNVARMGKF
                     SSDRTIREYAREIWHLAPVPPEAAPR"
     gene            complement(3589..3930)
                     /locus_tag="P5164_07600"
     CDS             complement(3589..3930)
                     /locus_tag="P5164_07600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRY43796.1"
                     /translation="MNLTIEPRESHVLARIEDTRLDALSAPDFKARMSGALTGTAPKV
                     VLDLSPVAFIDSAGLASILSLVKGLPEGGDLRIAGARDAVRAVFRLTRLDKVLALHDT
                     VEDAVAAVGAR"
     gene            4143..5540
                     /locus_tag="P5164_07605"
     CDS             4143..5540
                     /locus_tag="P5164_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HRY43797.1"
                     /translation="MPGALLLAAALAAVPSAAPPAPAPEALHAAWPGVRLSPPSGSYA
                     PEALRADLEALAGSSRGVVRIAEKGRSTEGREIAVVLAGSGPEKVLLWSQMHGDEPTA
                     TAALVDLLAFLAENGAEPATAHLLTRLTLVVVPMLNPDGAVRGDRRNAQGIDINRDAS
                     RLQSPEGRFLKELRDRYAPSAGFNLHNQSPLVTAGPGGPQVAIAVLAVSASRNEPDGP
                     SLARKRGLAGHLARVASTFVPGRVTRYATDYTERAFGDSMSRWGTPTVLLETGGWHGP
                     REEERLVRLNFVLLLSALHALARGEETADAPFYETLPPNVRGDLVDLVLRGATVHGGR
                     GLPPFLSDVAVVKARAFAGDGRRLSSAAVVDVGDLGHLAGLEELDAAGLVLAPAPVGG
                     AEAWEKVVSDLAARGLAKDGAFLLDDAALAREARAWAAGRAIVPWATVDLVLFRKGEK
                     GLVVEGFVREGSRTK"
     gene            complement(5560..6021)
                     /locus_tag="P5164_07610"
     CDS             complement(5560..6021)
                     /locus_tag="P5164_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRY43798.1"
                     /translation="MNVARSGPAVLLAVGAAVGCASAPPKAPAAALREEVFATERAFA
                     KTMADRDHAAFASFLASETIFFSGEKPLRGAEAVAAWWKKYYEGKEAPFSWEPAQVEV
                     LDSGTLALSTGPVRDPTGKQTGTFTSIWRREGPGTWRIVFDKGNPVCPTEP"
     gene            6279..6665
                     /locus_tag="P5164_07615"
     CDS             6279..6665
                     /locus_tag="P5164_07615"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HRY43799.1"
                     /translation="MPGLDREKHAAFWKAGAEEDLAAASRLLESGHRRHGLFFAHLAV
                     EKALKRLATLRTGEVPPKSHDLLRLAAVAGLAVDEERGVSMARLGRFCMEGRYPESWA
                     PPPSPELATEVLEQAKGLVAWLSSMS"
     gene            6644..6988
                     /locus_tag="P5164_07620"
     CDS             6644..6988
                     /locus_tag="P5164_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRY43800.1"
                     /translation="MAELDVLAKIRLYLETVQRLGIPVARAVLFGSRARGTAGEWSDI
                     DLVVVSPVFDGPDGPGAVDTLWRACAATGGYIEPVACGVKRWEEDDSSPLLAVARAEG
                     IDVLGAARVAGL"
     gene            complement(7017..8417)
                     /locus_tag="P5164_07625"
     CDS             complement(7017..8417)
                     /locus_tag="P5164_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRY43801.1"
                     /translation="MSEARLPDSTRRPLGKLPARVSPVGFGGYRVDDRVARHREALRL
                     ALLSGVNLVDTSTNYGDGHSELLVGKVLREMAAAGEVRREDVVVVTKAGYVQGQNLRD
                     AHQRRKLGDPWPELTEYSRDCWHCISPPFLKDQLSASLGRLAMPRVDLLLLHNPEYFL
                     ADAEHRNLPLAEARDTFYDRAGRAFEQLEKEVAAGRIGAYGVSSNTFVVPHEKDDAVD
                     LLRLLDLAGPGFAAIQLPMNPVELGAREPIHTSDGRSVLDVAREHGLAVLVNRPLNGF
                     RQGRLHRFATVGAPFPPVPSFAPDALARLRSAEEEFALVYAPRLSVEDGVAPPEELLA
                     VSPLLAEFEKRGLDLSTFREVWDDVVEPRRTELLSELREVFDRDADFGRWADGWDAAL
                     LEAASALVARLLPKEEARAADLSPIVTRDLHESPEGTLSQRTIKGLADVEGVTSVLVG
                     MRRPEYVRDVVGAFGG"
     gene            8504..9811
                     /locus_tag="P5164_07630"
     CDS             8504..9811
                     /locus_tag="P5164_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY43802.1"
                     /translation="MSSTRPEKPWNPVELEVELFCRGLLVDESARADVQARRVARTRA
                     GLGSGLELVIPAPRQGVWVNVPLDEPFVVSSPLTLHKAGESFAVRDARLPDVVYPVRL
                     PDPPAWYARSTKRGVPMTRVGVLQGTYLGVYFAETCMYWYNTPSAANCKFCTTGVNVG
                     TNEELAKKVEDVVEVAKVAREESGVTFTHFNTGYHYEDDPKKRPMHGLVQARPFVSAI
                     RRNVGGFIGVQAVPVLRDCWDEYDELIELGCDHFSFCVELLDEKWFEEVCPGKATTVG
                     QDAFFEALEYTAKKLGRGRVSGEIIAGLEPIESTMRAIDRIVAGGGFPTVCIFRPLAG
                     SNLAHLPSPDPGEMREVMAYQYEAARRAGLAVGILPNIKVSLVVQPEEGRELLAEKSW
                     LDPYEWKVAALRALARPYAAWRKRPRPDAPEFRPEIPGTSAVG"
     gene            9847..>10204
                     /locus_tag="P5164_07635"
     CDS             9847..>10204
                     /locus_tag="P5164_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRY43803.1"
                     /translation="MKWSYTVARVSGIDIRIHATFALILALGAFQWGFPKGFAGESSF
                     AGAVFGMGLMILLFACVVLHELGHSLVAQRFGLTVREIVLLPIGGVARLEKNPEKPQH
                     ELLIALAGPLVNVAIAV"
BASE COUNT         1388 a         3603 c         3744 g         1469 t
ORIGIN      
        1 ccgcccgtgg atgtacgagg tctcgatggc gacgtggagg acgaacctcg accccttcgc
       61 gcgctggagc cggagcgaga agtcgagcgc gttcttgcag ccgaggacgt tcgcccggaa
      121 ggagctctcg taggtgtcgt cgaaggagac cgaggcggcg cagtggacga cgtgcgtgac
      181 cgtccgctcg agcgtcgcga gctcgtcctc ggggatcccg aggcccggct tctcgatgtc
      241 cccctcgacg aaccggaact tgcgcgccgc gcgcccgccg acgccgagcc gcgagaggag
      301 caggcggccg cgctccgtcg gggagaggac gcggatcacc tcacgcgtct tcgggtcgcg
      361 aaccgtctgg ggccgaatga cggagacgac ctccgcgatc ctccggtccg aggccgcctg
      421 ggcgaggatc tcctggccga ggaagccggt cgcgccggtc aggaggacgc ggagccgcgg
      481 gcttcgcccg cgggcggcga gggccgcgcg ggcgtcggcg cgtagcctct cgatccgctg
      541 cgacacgtcg cgcgccgtcc ggtgcgtcgg ctcgcggaac tggaagaaga cggagcggtc
      601 gacgacgccg atcagcttct tcgccccccg ctccaccgcg tgcgagccgg tgaggcggcc
      661 gagccggccg gcgaccctca ggccgaacgc gttcgccgac ttcatcgcct cgcgcacctt
      721 ctcctcgttg ccggtcacct ggacgaggcg gctcgcgagg aaggaggtcg tcacctcgaa
      781 cgggttcttg tcgcggtgcg ccgtgatgag gagctcctcc gggtcgaccg gccggaggac
      841 ctcgcgcccc gtcgtcgcgc tcattcgggc gatgtagcgg gacggcgagc tctcccagag
      901 gaactccccc tcggggtgga cgtgcggcgt cccgtcgacg atcggcttcg gggcggcggc
      961 cggccgcttc ggtcgcgccg ggcggcgtgc cgcagggggg gcggcattcg aggcggcggc
     1021 aggggccgcc cggcgtcggc ggggcgcggc attcgtcatt ggactcctcc ggggagtctg
     1081 attgtccccc gaacggccgc gggaaccgaa attcgtgcta gcgcggcgcc gcctccggcg
     1141 ggacgggcgc gaggtgccag atctcccggg cgtactcgcg gatcgtccgg tcggaggaga
     1201 acttccccat ccgcgccacg ttcagcaccg ccttgcgcgt ccacccggcc gcgtccgtcc
     1261 actggtccgc gacccgctcc tgcgccgtgg cgtaggcgtc gaagtcggcg aggtggaagt
     1321 acgggtcgtg gttctcgacg acgttctcga agagccagga gaagatcccc ggctcgaacg
     1381 gagcgaagag gggcgtccgg aacgcgtcga ggacgcgccg gacgcgcggg tcgtcctcgt
     1441 agacgcgccg ggcggagtac gccccgggct gcttcagacg gtcgagctcc tccgcggtgt
     1501 ggccgaagat gaagatgttc tcctcgcccg cctcctccat gatctcgatg ttcgcgccgt
     1561 cgagcgtccc gatcgtcagc gcgccgttca tcgagagctt catgttgccg gtgccggacg
     1621 cctccatccc ggcggtcgag atctgctcgg agaggtcggc cgccggaatg atccgctcgg
     1681 cgagcgagac ccggtagtcg gggaggaaga cgcacttgag catccccttc acccgcgtgt
     1741 cccggttgac cctctccccg acggcgtgga tcagcttgat gacctgcttg gcgatccagt
     1801 agccgggcgc ggccttccct gcgaagacga acgtccgcgg cgccttcggc acctccccgt
     1861 cctcgacgat ccggaagtag aggtggacga cgtggagggc gttcaggagc tgccgcttgt
     1921 agaggtggat ccgcttgatc tggatgtcga agagggagtc ggggtcgacc cgcacccggg
     1981 tcaggtcggc gacgatcctc gcgaggcgga ccttgttctc gcgtttgacg gcgcggaagc
     2041 ggtcgcggaa cgcggcgtcc tcggcgagcc ggtcgagcgg ctcgcgcagg aggccgtagt
     2101 cggtcaccca gccggggccg gcggtctcgg tgaggagggc ggcgaggctc gggttcgact
     2161 tcaggagcca gcgtcgcggc gtgacgccgt tcgtcttgtt gttgaaccgc tccgggtgga
     2221 ggcggtagag gtcggggaag aggttctcgg tgatgaggcg ggagtggagg gccgcgacgc
     2281 cgttgatgga gtggctcccg gccgccgcga gctgagccat ccggacctgc ttcggctctc
     2341 cctcctcgat catcgagacg cgggcgagga cctcctggtc gcccggcgcg cggtgcgcca
     2401 cctcgtcgag gaagcggcgg ttgatctcgt agatgatctg caggtgccgc ggcaggaccg
     2461 tctcgacgag cgggagcggc cacttctcga gcgcttccgg cagcagggtg tggttcgtgt
     2521 agccgagcgt ggcgacggtc atctcccacg cccgctccca cgccacgtcg tgctcgtcga
     2581 cgaagagccg catcagctcg gccacggcga gggcggggtg ggtgtcgttc agctggatcg
     2641 cgatccggtc ggggaacgcc gcgatgggat gcccctcgcg gaggaagcgc cggaggatgt
     2701 cccgtagggc gcaggcgacg aagaagtact cctgcaggag gcggagctcc ttccccttct
     2761 ggaccctgtc ggaggggtag aggaccttgg agatcgtctc cgacccgatc gactcctgga
     2821 ccgcctggac gtagtcgccg gagttgaaga actccatgtt gaactcgtcg gaggcgcgcg
     2881 cggagtagag ccggagcgcg ttcaccgtct cgccgccgta gccgacgacg ggcatctcgt
     2941 gcggcacgcc gacgaggatc ttccagtcca tccacatcgg gttgtactgg ctcttccggt
     3001 cgaccgtgtc gacgatccgg ccgtagaccg ggacgaggac ggcctcctcg ggccgctcga
     3061 cggtccaggg catcgcggcg tggagccagc ggtcggggcg ctccttctgg tagccgttgt
     3121 cgatctcctg gcggaagagg ccgtactggt agaagaggcc gtagccgtag cccggcaggt
     3181 ggagcgtcgc gagcgagtcg aggaagcagg cggcgaggcg gccgaggcct ccgttgccga
     3241 gcccggcgtc gacctcctcc tcgcgcacct ggtcgaggcc gtgccccagc tcggcgagcg
     3301 cctggacggc cgcgtcgtgg aggccgaggt tgtggaggga gtcgacgagc gagcgcccga
     3361 tcaggaactc catcgagagg tagtagaccc gcttggcgcc ggcctgccgg tagcgcttct
     3421 ccgtcgcgag gaggcgctcc caggcccggt cgcggacggc gagcccgagg gcctggaaga
     3481 ggtcgccgtt cgtcgcgtcc tcccaggcct ttccgagcga gtgccggacg tgccagcgga
     3541 tcgcctcgcg cagctcctcc ccggtccgcg gcagcggcgg ggcgacgctc atcgggcccc
     3601 cacggcggcg accgcgtcct ccaccgtgtc gtggagcgcg agcaccttgt cgagccgcgt
     3661 caggcggaag acggcgcgga cggcgtcgcg cgctccggcg atccggaggt cgcctccctc
     3721 ggggagcccc ttcacgagcg acaggatgga ggccagcccg gcgctgtcga tgaacgcgac
     3781 gggcgagagg tcgaggacga ccttcggcgc cgtcccggtg agcgcgcccg acatgcgcgc
     3841 cttgaagtcg ggggcggaga gggcgtcgag gcgggtgtcc tcgatccggg ccaggacgtg
     3901 agactccctc ggctcgatcg tcaggttcat cgttcctccc tcggctccgt ccggagaccc
     3961 ggcgaggggt cccctcccgg cgagccccgc gcgaaccggc ggaaccgctc cccggcacgc
     4021 cgcgggagga gccttcaggg aaccgaaccc ctttcgtccc ggacgcggtc agcttgccac
     4081 tcgtggcgga ggtgtcaaga agggcgctgc cccgcggcgt cgatcgctgg taccgtcccg
     4141 ccatgccggg agcgctcctc ctcgccgccg ccctcgccgc agtcccctct gccgcgccgc
     4201 ccgctcccgc accggaggcg ctccacgccg cctggccggg cgtgcggctc tcgcccccgt
     4261 ccggctcgta cgcccccgag gcgctccggg ccgacctcga ggcgctcgcg ggcagctccc
     4321 gcggcgtcgt ccggatcgcg gagaaggggc gctcgaccga gggccgggag atcgcggtcg
     4381 tcctcgcggg aagcggcccg gaaaaagtcc tcctctggtc gcagatgcac ggcgacgagc
     4441 cgaccgcgac ggcagcgctc gtcgacctcc tcgcgttcct cgccgagaac ggggccgagc
     4501 ccgccaccgc gcacctcctc acgcgcctca ccctcgtcgt cgtcccgatg ctcaacccgg
     4561 acggcgccgt gcgcggcgac cggaggaacg cgcaggggat cgacatcaac cgagatgcct
     4621 cgaggctcca gtcgcccgaa gggcgcttcc tcaaggagct ccgcgatcgc tacgcgccgt
     4681 cggccggctt caacctccac aaccagagcc cgctcgtcac cgccggtccg ggggggccgc
     4741 aggtggcgat cgccgtcctc gccgtatcgg cctcccggaa cgaacccgac gggccgtccc
     4801 tcgcacgaaa gcggggcctg gcgggacacc tcgcccgggt cgcctcgacg ttcgtgccgg
     4861 gccgagtcac ccgctacgcc accgactaca ccgagcgcgc cttcggcgac tcgatgtcgc
     4921 gctgggggac cccgaccgtc ctcctcgaga ccggcggctg gcacggcccg cgggaagagg
     4981 agcgcctcgt gaggctcaac ttcgtcctcc tcctctcggc gctccacgcc ctcgcgcgcg
     5041 gcgaggagac ggcggacgcc ccgttctacg agacgctccc gccgaacgtc cgcggcgacc
     5101 tcgtcgacct cgtcctgcgc ggagccaccg tccacggcgg gcgcggcctc ccgcccttcc
     5161 tctccgacgt cgcggtcgtg aaggcgcgcg ctttcgcggg cgacggccgg cggctctcct
     5221 cggccgccgt cgtcgacgtc ggcgacctcg ggcacctcgc gggcctcgag gagctggacg
     5281 ccgccggcct cgttctcgcg ccggccccgg tcggaggggc tgaagcgtgg gagaaggtcg
     5341 tctccgatct cgccgcacgg ggcctcgcga aggacggagc gttcctcctc gacgacgccg
     5401 cgctcgcgcg cgaagcccgc gcctgggccg cggggcgggc gatcgtcccg tgggcgacgg
     5461 tcgatctcgt tctcttccgg aagggggaga aggggctcgt cgtcgaggga ttcgtccgcg
     5521 aggggagccg gacgaagtaa cggccgtcgt cccgacgggt cagggctccg tcgggcagac
     5581 cgggttcccc ttgtcgaaga cgatccgcca cgtcccgggg ccttcgcgcc gccagatcga
     5641 cgtgaacgtg ccggtctgct tccctgtggg gtcgcggacc ggtcccgtgc tgagcgcgag
     5701 cgtccccgag tcgaggacct cgacctgcgc gggctcccag gagaaggggg cctccttccc
     5761 ctcgtagtac ttcttccacc aggccgcgac cgcctccgcg ccgcggagcg gcttctctcc
     5821 ggagaagaag atcgtctccg aggcgaggaa cgacgcgaac gcggcgtggt cgcggtcggc
     5881 catcgtcttc gcgaacgcac gctcggtcgc gaagacctcc tcgcggagag ccgcggcggg
     5941 ggccttcggc ggggcgctgg cgcagcccac ggcggcgccg acggcgagaa ggacagccgg
     6001 tccggaacgg gcgacgttca tcgatacctc cgaaggattc gcgccggacc ttatcgcggc
     6061 cggggccgca gagccacgag cactccacga ggaggaacaa cctcgccctc gcccatcgca
     6121 ccagcctctc gcacgccggg aggtgaacgg tggcggcgcc ggtctgaagt ccgaaggcgg
     6181 tcgcatcccg gggacgccgc tcgtgaaacc ggcatggggc ggtcccgggc gggtgcggca
     6241 gacgggagcc ggcgacccgc gagtgctaga gtttctccat gccggggctc gatcgcgaga
     6301 agcacgccgc gttctggaag gcgggcgccg aggaggatct cgccgccgcc tcgcggctcc
     6361 tcgaatcggg acaccgccga cacggcctct tcttcgccca ccttgccgtc gagaaggcgc
     6421 tgaagcggct cgcgacgctc cggacgggcg aggttccgcc gaagagccac gacctgctcc
     6481 ggctggccgc cgtcgcagga ctcgccgtgg acgaggaacg tggcgtgtcg atggcgcgcc
     6541 tcgggcgttt ctgcatggag gggcggtatc cggagtcgtg ggcgccgccg ccgtctccgg
     6601 agctggcaac ggaggtcctc gaacaggcca aggggctcgt cgcgtggctg agctcgatgt
     6661 cctagccaag atccggctct acctggagac ggtccagagg ctggggatcc ccgtggcgcg
     6721 agccgtcctc ttcgggtccc gggctcgagg caccgccggc gagtggagcg acatcgacct
     6781 cgtcgtcgtc tcgccggtct tcgacggccc ggacgggccc ggtgcggtcg acacgctctg
     6841 gcgggcctgc gccgcgacgg ggggctacat cgagcccgtg gcctgcggcg tgaagcgctg
     6901 ggaggaggac gacagctctc ccctcctcgc cgtcgcgcgt gcggagggga tcgacgtcct
     6961 cggtgcggcg cgggtcgcag gcctctgaat ttgggcttcg aggattcgct cccgccctac
     7021 cccccgaacg ctcccaccac gtcccgcacg tactcgggcc ggcgcatccc gacgaggacg
     7081 ctcgtcacgc cctcgacgtc cgcgaggccc ttgatcgtcc tctgcgagag ggtcccctcg
     7141 ggcgactcgt ggaggtcgcg ggtgacgatc ggcgagaggt cggcggcgcg ggcctcctcc
     7201 ttcggcagga ggcgcgcgac gagcgcggag gcggcctcga ggagggccgc gtcccagccg
     7261 tcggcccagc ggccgaaatc ggcgtcgcgg tcgaagacct cgcgcagctc cgagaggagc
     7321 tcggtgcgtc gcggctcgac gacgtcgtcc cagacctcgc ggaaggtcga gaggtcgagg
     7381 ccgcgcttct cgaactcggc gaggaggggc gagacggcga ggagctcctc gggcggcgcg
     7441 acgccgtcct ccaccgagag gcgcggcgcg tagacgagcg cgaactcctc ctccgccgag
     7501 cggaggcggg cgagggcgtc gggcgcgaag gaggggacgg gcgggaaagg cgcgccgacc
     7561 gtcgcgaagc ggtggagccg cccctgccgg aacccgttca gcgggcggtt cacgaggacg
     7621 gcgaggccgt gctcgcgggc gacgtcgagg acgctccggc cgtcggaggt gtggatcggc
     7681 tcgcgcgcgc cgagctccac cgggttcatc gggagctgga tcgccgcgaa gccggggccg
     7741 gcgaggtcga ggaggcgaag gagatcgacg gcgtcgtcct tctcgtgcgg gacgacgaac
     7801 gtgttcgagg agacgccgta ggcgccgatc cgccccgcgg cgacctcctt ctcgagctgc
     7861 tcgaaggccc ggccggcccg gtcgtagaac gtgtcgcgcg cctcggcgag cggcaggttc
     7921 cggtgctcgg cgtccgccag gaagtactcg gggttgtgga ggaggaggag gtcgacgcgc
     7981 ggcatcgcga ggcgcccgag cgaggcggag agctggtcct tcaggaacgg cggggagatg
     8041 cagtgccagc agtcccgcga gtactccgtc agctcgggcc acgggtctcc gagcttgcgg
     8101 cgctggtgcg cgtcgcggag gttctgcccc tggacgtagc ccgccttcgt gacgacgacg
     8161 acgtcctcgc ggcggacctc gcccgccgcg gccatctccc ggaggacctt cccgacgagg
     8221 agctcgctgt gcccgtcgcc gtagttcgtc gaggtgtcga cgaggttcac gccggagagg
     8281 agcgcgagcc ggagcgcctc ccggtgccgg gcgacccggt cgtccacccg gtagccgccg
     8341 aacccgaccg gggagacgcg ggcggggagc ttcccgaggg ggcggcgggt cgagtcgggg
     8401 aggcgggcct cgctcatggc tcgggacgat atcggacgcg ggccgattct tcccgccccg
     8461 ggcccgcctt cggcgctctt gccgcacctg cgagaatcgg ccgatgtcca gcactcgccc
     8521 cgaaaagccc tggaaccccg tcgagctcga ggtcgagctc ttctgccggg ggctcctcgt
     8581 cgacgaatcc gcccgggccg acgtccaggc ccgccgcgtc gcccgcaccc gcgccggcct
     8641 cggctcgggc ctcgagctcg tcatccccgc cccgcggcag ggggtctggg tcaacgttcc
     8701 gctcgacgag ccgttcgtgg tctcctcccc gctgacgctc cacaaggcgg gggagtcgtt
     8761 cgccgtccgc gacgcccgcc tccccgacgt cgtctacccg gtccgcctcc ccgacccgcc
     8821 ggcctggtac gcccgctcga cgaagcgagg ggtcccgatg acccgcgtcg gcgtcctgca
     8881 ggggacctac ctcggcgtct acttcgccga gacctgcatg tactggtaca acaccccttc
     8941 ggcggcgaac tgcaagttct gtacgaccgg cgtgaacgtc gggacgaacg aggagctcgc
     9001 caagaaagtc gaggacgtcg tcgaggtggc gaaggtcgcc cgcgaggagt cgggggtcac
     9061 attcacccac ttcaacaccg gctaccacta cgaggacgac ccgaagaagc ggccgatgca
     9121 cggcctcgtg caggcgcggc ccttcgtctc ggccatccgg cggaacgtcg gcggcttcat
     9181 cggagtccag gcggtcccgg tcctgcggga ctgctgggac gagtacgacg agctgatcga
     9241 gctcggctgc gaccacttca gcttctgcgt cgagctcctc gacgagaagt ggttcgagga
     9301 ggtctgcccg ggcaaggcga cgaccgtcgg gcaggacgcg ttcttcgagg cgctcgagta
     9361 cacggcgaag aagctcggcc gcggccgcgt ctccggcgag atcatcgcgg ggctcgagcc
     9421 gatcgagtcg acgatgcgcg cgatcgaccg gatcgtcgcc ggcggcggct tccccacggt
     9481 ctgcatcttc cggcccctcg ccgggagcaa cctggcgcac ctcccgtctc cagacccggg
     9541 cgagatgcgc gaggtgatgg cctaccagta cgaggcggcc cgccgcgcgg gcctcgccgt
     9601 cggcatcctc ccgaacatca aggtctccct cgtcgtccag ccggaggagg ggcgcgagct
     9661 cctcgcggag aagagctggc tcgacccgta cgagtggaag gtcgcggcgc tccgagctct
     9721 cgcccgcccc tacgccgcct ggcgcaagcg cccccgcccc gatgcccccg agttccgccc
     9781 cgaaatcccc ggcacatcgg cggtcggata agggatgacg gacggcgtgt ccgccgggag
     9841 cccgccatga agtggtcgta caccgtcgcc cgcgtctcgg gcatcgacat ccgcatccac
     9901 gcgacgttcg cgctgatcct cgcgctcggc gcgttccagt ggggcttccc gaagggtttc
     9961 gccggggagt cctcgttcgc gggcgccgtc ttcgggatgg gcctgatgat ccttctcttc
    10021 gcctgcgtcg tcctgcacga gctggggcac agcctcgtcg cgcagcggtt cggcctgacg
    10081 gtgcgggaga tcgtcctcct gccgatcggg ggcgtcgcac gcctcgagaa gaaccccgag
    10141 aagccgcagc acgagctcct gatcgccctc gccgggccgc tcgtgaacgt ggcgatcgcc
    10201 gtcc
//
LOCUS       DAOTEZ010000111        10182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4638_length_10182_cov_5.910635, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000111 DAOTEZ010000000
VERSION     DAOTEZ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10182
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4638_length_10182_cov_5.910635"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="P5164_07640"
     CDS             <1..459
                     /locus_tag="P5164_07640"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HRY43804.1"
                     /translation="VVASPRPKAILEMNAVKSLLDAGFVVVAAGGGGIAVVPDEKGEL
                     RGAAAVIDKDFASSLLARELGADLFVISTAVEKVCLDYGKPEQKDLDRMTVAEAKQYC
                     AEGHFKPGSMLPKIQAVIEFLEGGGKEAIITDPEHLGLALAGKTGTHVVP"
     gene            481..1680
                     /locus_tag="P5164_07645"
     CDS             481..1680
                     /locus_tag="P5164_07645"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HRY43805.1"
                     /translation="MNATPVFAEATLLRNPFARPETRWPAPDPSVWTFHRRMPAYRAT
                     TLYDCPELAAKYAVKRVLVKAETQRLGLPSFKILGASWASYRALCDHLGFEPEPWANI
                     NDLSRRIAYLRPFSFAAATDGNHGRAVAFMARLLGFSCRIFVPKGTVPARIHAIQNEG
                     AEVTVVDGDYDAAVARSAEEAGESCLVISDTSWPGYDVTPRRVVDGYTTIFMEIEQAL
                     AAAGVGAPDVVIVPMGVGAFMSAAVSYYRSRPWNGALVGVEPADANCIQASAGAGEMT
                     AVPGPHRSIMVGLNCGLPSPVAWPRVSTGVEWMVSIGDERAREAMRGLAGAGVVAGET
                     GAAALGALAALAEDGATAAFREAGLLGPDKTVLLMVTEGATDRGNYQAVVGRTPEEVG
                     TILTVAR"
     gene            1739..4360
                     /gene="xdh"
                     /locus_tag="P5164_07650"
     CDS             1739..4360
                     /gene="xdh"
                     /locus_tag="P5164_07650"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0046415 - urate metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HRY43806.1"
                     /translation="MSTTQLERPKDAAPIEPALHAFRVNGKEVATPKDVKLLTFLREE
                     LDLTSVKNGCSEGSCGACMVLVDGKPVRSCLTTTAKVAGKSVVTVEGLSEREKDVYAW
                     AFANAGAVQCGFCMPGMVMTAKGLVDANPEPATEEVAKALQFSMCRCTGYVKIEKGIQ
                     EAARLLREGEAVPGEHDVPLSVGAKILRADAREKVLGGAVYVDDMKVPGMLHGAVLRA
                     PKARVKVLGVDASEALAMPGVVAVMTAADVPGERLQGHIIHDWPAMIAVGEETRYVGD
                     AIALVAAETREIAKEAAKRIRLDHEVLPPLVDARRALDDDAPKLHPKGNLLAKTVLKR
                     GDPDRAIAEAAHVIRKTFRTPETEHAFMEPESALAIPNGDGTMTVYTGTQSIYDDHKG
                     IVGVLGVPPESVKVISAYVGGGFGGKEDLIVQHHAALLAWKTGAPVKVTLDRQESIRV
                     HPKRHAMEMEYVVAADAEGRVTAVKANLLEDTGAYASLGGPVLQRACTHGAGPYKIPN
                     VEMVGTGVYTNNPPGGAFRGFGVTQSCFAIESCLNLLAKEVGVSPWEIRWRNAVEPGD
                     VLPNGQVCDDGTALKECLLAVKELCETRPDAGIAVALKNAGIGVGLPDIGRVRLKVES
                     GKAVIYTSAACMGQGLASVLMQFVAEITGLDWERVVVATPDTSTSPNSGTTTASRQTL
                     FTGEAACRAARALKADLDGSTLEALEGKEYHAEFSGVTDPFASDKPNPVSHVAYSYAA
                     HVVLLDGEGMLEKVVAAHDIGRAINPVQVESQIEGGVVMGLGYALTEDFPLKEGEPQV
                     KYGTLGLFKAAKVPPIESVIVEKNPSPLARGAKGLGEIATIPTAPAVAGAYFLRDGLF
                     RTELPLAGTPYSRRKTG"
     gene            4357..5388
                     /locus_tag="P5164_07655"
     CDS             4357..5388
                     /locus_tag="P5164_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HRY43807.1"
                     /translation="MSLALLEVLLAAREPLVLCTVIQVKGSAPRHPGSWMLAGPAGLV
                     AGSVGGGGGEAVVLDAAREALGDGKARVLEVEKLGNAAEGPEMVCGGTALILVEPLGS
                     KSAYAAARELLSRGERALLARRTATGESAAMGRSGVWSPAPPAGADPEAARRALEHGL
                     PLLDEAAGVFYDPLVPPERLLVLGGGHVGRAVAAAAHAVGFAVTVADDRAEFLAPERY
                     PEGVQTHHGSYTGAVGAFPWDASAYAVIVTRGHLADLESLRAVLGKPWRYAGMIGSRK
                     KVRLMLDQAIADGFDPERVAAVNAPIGLDIGAETPEELAVSIVGELVAARRGVAAASL
                     ARRRPRSAS"
     gene            5436..6155
                     /gene="yqeC"
                     /locus_tag="P5164_07660"
     CDS             5436..6155
                     /gene="yqeC"
                     /locus_tag="P5164_07660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HRY43808.1"
                     /translation="MSFVGAGGKTSLLLHLARELAADSRPVLVTTTTHLADPREEPGA
                     FVERVLLLPALEHPDPDAPLPAPTPGVTLLVSRTAEPPRKLKGIDPSHVSRLASVWPV
                     VLVEADGSKRLPIKSPAGHEPVVPAETGLTIGVLGLDALGWPMDERTVHRPERFAAVT
                     GCLPGAAIGWDHVVALARHPEGLFKGARGARVALLNKVDESPYLPSPGQLRALGADLV
                     LLSGADEAERAFFTVPQGPAT"
     gene            6152..6946
                     /gene="yqeB"
                     /locus_tag="P5164_07665"
     CDS             6152..6946
                     /gene="yqeB"
                     /locus_tag="P5164_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HRY43809.1"
                     /translation="MSLAGGRLVVLRGAGDLATGCIVRLVRSGFRVVALELPRPSAIR
                     RTVSFSEAVYDGSAEVEGIRAVRHDEPPAAWDAATVPILVDPEARLVPRLAPLAVVDA
                     ILAKRNLGTRIDLAPVVVALGPGFEAGRDAHAVVETNRGHALGRVLLAGRAEDDTAVP
                     GTIAGHAGDRVVRAPAAGEVVALRAIGDLVAAGEPVLTVGGAVARASIGGVLRGLIRP
                     GYPAPAGLKVADVDPRGVREHCFTVSDKARAVAGGVIEALLALAPG"
     gene            complement(6963..7877)
                     /locus_tag="P5164_07670"
     CDS             complement(6963..7877)
                     /locus_tag="P5164_07670"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRY43810.1"
                     /translation="MVASGEPQEIRERLKEVYHDLPEAMRRIADALMSDPLLGALWGI
                     ESLAERANVSIGTVVRFSKRLGYRGFSEFRDALREACQIRSGGDPLEALEAPADPFWA
                     LGEVVRRDRENLARLVQTLDHPTLDSATQLLTRARHRVLLGRGVSNLMCQVLAFNLTQ
                     AGLPCIAASPSDFSNQVANLGAGDLLVVVSCAPFSRETVDAAAFARRCEIPVLAFTDR
                     HDSPLTPSATVAIVLPTEDFLFSFGLSAFSVVSHALAIAVAAKDTAGTSNRLKAADEV
                     AQPLFVEHWLPMQPGAIRVERPAKPKAD"
     gene            complement(7958..8896)
                     /locus_tag="P5164_07675"
     CDS             complement(7958..8896)
                     /locus_tag="P5164_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43811.1"
                     /translation="MNATHVSLALLLGAALAAAPAEAQSKKKKPAVTKPAAAPKITPA
                     NARMTALQDRRSNGHFPRCTLGMELPDYPAHEVTAARVVVRKAVDDLGTDLVKEDAAE
                     VPLEPTQRGQFGKPEGGKAAPPTIVFAEMKNPPRAAKALKEVSGEIELFVPSRDPNGE
                     ARIERFLALAGKAAVHPALRANGVEISFVSKAQLDAERKKAEEAETAKLKKEGYEDAD
                     SIRSMVESALYSFPKGEEREVILKVKDPKKAIQEIKAFGANGERVFGTDSEESGFRVL
                     TFWNEPPQADWTLSVQMRSDSSLVRHTFAFRDVPLP"
     gene            complement(8893..9798)
                     /locus_tag="P5164_07680"
     CDS             complement(8893..9798)
                     /locus_tag="P5164_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43812.1"
                     /translation="MVRSAVRRAVALGAFLALGAAGTTEAQTNVKVVAKEVVDDRISE
                     GLMKGGLVLQLDLEGDGLDVVQSARVRLKSAKADTGEDLLPKEEKAPDFSDRNVNAGT
                     LQVVVNSPPRGASTVRVAGTVELFVPGRDASAVVRMPGALAKLDKPVVSKGLKSAKVE
                     LTLLSRAKYVEERKKHRLDEAKIAKIREEAKARGMKDEETDALVEMAKAFDEMGEGDL
                     PEHAFYLKVPAASEEKIQEIWLETAKGEKLESGGSSSRSDEAFVLKQVALKAAPPKDA
                     VLVARLLTDKSIVSVPFEMKEVRLP"
     gene            9917..9992
                     /locus_tag="P5164_07685"
     tRNA            9917..9992
                     /locus_tag="P5164_07685"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9950..9952,aa:Lys,seq:ttt)
     gene            10106..10182
                     /locus_tag="P5164_07690"
     tRNA            10106..10182
                     /locus_tag="P5164_07690"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10140..10142,aa:Glu,seq:ttc)
BASE COUNT         1408 a         3811 c         3546 g         1417 t
ORIGIN      
        1 gtcgtcgcct cgccgaggcc gaaggcgatc ctcgagatga acgccgtcaa gagcctcctc
       61 gacgccgggt tcgtcgtcgt ggcggccggc ggcggcggga tcgccgtcgt ccccgacgag
      121 aagggcgagc tgaggggagc cgccgccgtc atcgacaagg acttcgcctc gagcctcctc
      181 gcacgagagc tcggcgccga cctcttcgtc atctcgacgg ctgtggagaa ggtctgcctc
      241 gactacggca agccggagca gaaggacctc gaccggatga cggtcgccga agcgaagcag
      301 tactgtgccg aagggcactt caagccgggc tccatgctcc cgaagatcca ggccgtcatc
      361 gagttcctcg aggggggcgg gaaggaggcg atcatcaccg accccgagca cctcggcctc
      421 gccctcgccg ggaagaccgg gacccacgtc gtcccctgac cgtcagccca ggaggccccc
      481 atgaacgcca cgcccgtctt cgcggaagcg acgctgctca ggaacccctt cgcccggccc
      541 gagacccgct ggcccgcgcc cgacccgtcg gtctggacgt tccaccggcg gatgccggcc
      601 taccgggcga cgacgctcta cgactgtccc gagctcgcgg cgaagtacgc cgtgaagcgc
      661 gtcctcgtga aggccgagac gcagcgcctg gggctcccca gcttcaagat cctcggagcc
      721 tcgtgggcgt cgtaccgggc gctctgcgac cacctcggct tcgagccgga gccgtgggcg
      781 aacatcaacg acctctcgcg ccggatcgcc tacctgcgcc ccttctcctt cgcggcggcg
      841 accgacggca accacggacg ggccgtcgcc ttcatggcgc ggctcctcgg cttctcctgc
      901 cggatcttcg tcccgaaagg gaccgtcccg gcccgcatcc acgcgatcca gaacgagggg
      961 gcggaggtga ccgtcgtcga cggcgactac gacgccgcgg tcgcccgctc cgccgaggag
     1021 gcgggcgaga gctgtctcgt catctccgac acctcctggc cgggctacga cgtgacgccc
     1081 cgccgggtcg tcgacggcta cacgacgatc ttcatggaga tcgagcaggc gctcgcggcc
     1141 gcgggcgtcg gcgcgcccga cgtcgtgatc gtcccgatgg gcgtcggcgc cttcatgtcg
     1201 gccgccgtca gctactaccg gagccgtccg tggaacgggg cgctcgtcgg cgtcgagccg
     1261 gcggacgcca actgcatcca ggcctcggcg ggggcgggcg agatgacggc cgtccccgga
     1321 ccgcaccgct cgatcatggt cggcctcaac tgcggccttc cgtcgcccgt ggcctggccg
     1381 cgcgtctcga cgggcgtcga gtggatggtc tcgatcggcg acgagcgggc gcgcgaggcg
     1441 atgcggggcc tcgccggcgc cggcgtcgtc gcgggagaga ccggcgcggc tgcgctcggc
     1501 gcgctcgcgg cgctcgccga ggacggtgcg accgccgcct tccgcgaggc gggcctcctc
     1561 ggcccggaca agaccgtcct cctcatggtg acggaagggg cgacggaccg cggcaactac
     1621 caggccgtcg tcgggcggac ccccgaggag gtcgggacga tcctcacggt ggcccgctga
     1681 gggccgctcc gaagcgggcc gcgaggctct agactggatt cgccacggga ggtgccccat
     1741 gtcgacgacg cagctggaac ggccgaagga cgcggccccg atcgagccgg cactccacgc
     1801 cttccgggtg aacgggaagg aggtcgcgac cccgaaggac gtaaagctcc tgacgttcct
     1861 ccgggaggag ctcgacctca cgtcggtgaa gaacggctgc tccgaggggt cgtgcggggc
     1921 ctgcatggtc ctcgtcgacg gcaagccggt ccgctcgtgc ctcacgacga cggcgaaggt
     1981 cgccgggaag agcgtcgtca ccgtcgaggg gctctccgag cgcgagaagg acgtctacgc
     2041 ctgggccttc gcgaacgcgg gcgccgtcca gtgcggcttc tgcatgccgg ggatggtgat
     2101 gacggccaag gggctcgtcg acgccaaccc cgagccggcg accgaggagg tcgcgaaggc
     2161 gctccagttc agcatgtgcc gctgcaccgg ctacgtgaag atcgagaagg ggatccagga
     2221 ggccgcgcgc ctcctccgcg agggggaggc ggtcccgggc gagcacgacg tcccgctctc
     2281 ggtcggcgcg aagatcctcc gggccgacgc gcgcgagaag gtcctcggcg gggcggtcta
     2341 cgtcgacgac atgaaggtcc cggggatgct ccacggggcc gtcctccgcg cgccgaaggc
     2401 gcgggtgaag gtcctcggcg tcgacgcgag cgaggcgctc gcgatgccgg gggtcgtggc
     2461 cgtgatgacc gcggccgacg tcccgggcga gcgactccag gggcacatca tccacgactg
     2521 gcccgccatg atcgccgtcg gcgaggagac gcggtacgtc ggcgacgcga tcgcgctcgt
     2581 cgccgccgag acccgcgaga tcgcgaagga ggcggcgaag cggatccgac tcgaccacga
     2641 ggtcctcccg cccctcgtcg acgcccggag ggccctcgac gacgacgcgc cgaagctcca
     2701 cccgaagggg aacctcctcg cgaagacggt gctcaagcgc ggcgacccag acagggcgat
     2761 cgccgaggcg gcgcacgtca tccggaagac cttccggacg ccggagacgg agcacgcctt
     2821 catggagccc gagagcgccc tcgcgatccc gaacggcgac ggcacgatga ccgtctacac
     2881 cgggacgcag agcatctacg acgaccacaa ggggatcgtg ggagtcctcg gcgtcccgcc
     2941 cgagagcgtg aaggtgatca gcgcctacgt cggcggcggg ttcggcggca aggaggacct
     3001 catcgtccag caccacgccg cgctcctcgc ctggaagacg ggggcgcccg tgaaggtgac
     3061 gctcgaccgc caggagtcga tccgcgtcca cccgaagcgg cacgcgatgg agatggagta
     3121 cgtcgtcgcg gccgacgccg aggggcgcgt gaccgccgtg aaggcgaacc tcctcgagga
     3181 caccggcgcc tacgcctcgc tcggtgggcc ggtcctccag agggcgtgca cgcacggcgc
     3241 ggggccgtac aagatcccga acgtcgagat ggtcggcacg ggcgtctaca cgaacaaccc
     3301 gcccggcggc gccttccgcg gcttcggcgt cacgcagtcg tgcttcgcga tcgagtcgtg
     3361 cctgaacctc ctcgcgaagg aggtcggcgt cagcccgtgg gagatccgct ggaggaacgc
     3421 cgtcgagccg ggcgacgtcc tcccgaacgg ccaggtctgc gacgacggga cggccctgaa
     3481 ggagtgcctc ctcgccgtga aggagctctg cgagacgagg cccgacgcgg ggatcgccgt
     3541 cgcgctcaag aacgccggga tcggcgtcgg cctccccgac atcggccgcg tgaggctgaa
     3601 ggtggaaagc gggaaggccg tgatttacac gagcgcggcc tgcatggggc aggggctcgc
     3661 ctccgtcctg atgcagttcg tcgccgagat caccgggctc gactgggagc gcgtcgtcgt
     3721 cgcgacgccc gacacctcga cctccccgaa cagcgggacg acgaccgcct cccgacagac
     3781 gctcttcacg ggcgaggcgg cctgccgggc ggcccgcgcg ctgaaagccg acctcgacgg
     3841 gtcgaccctc gaggcgctcg aggggaagga gtaccacgcc gagttctcgg gcgtcaccga
     3901 ccccttcgcc tccgacaagc cgaacccggt gagccacgtc gcctactcct acgcggccca
     3961 cgtcgtcctc ctcgacggcg agggaatgct cgagaaggtc gtcgcggcgc acgacatcgg
     4021 ccgggcgatc aatcccgtcc aggtcgagtc gcagatcgag gggggcgtcg tcatggggct
     4081 cggttacgcc ctcacggagg acttcccgct gaaggagggc gagccgcagg tgaagtacgg
     4141 cacgctcggc ctcttcaagg cggcgaaggt cccgccgatc gagtccgtca tcgtggagaa
     4201 gaacccgtcg ccgctcgccc ggggcgccaa gggactcggc gagatcgcga cgatcccgac
     4261 ggcccccgcg gtcgccggcg cctacttcct ccgcgacggc ctcttccgga ccgagctgcc
     4321 gctcgcgggg accccctaca gcaggaggaa gaccggatga gccttgccct cctcgaggtc
     4381 ctcctcgccg cccgcgagcc gctcgtcctc tgcaccgtga tccaggtgaa gggctccgct
     4441 ccgcgccacc ccggctcctg gatgctggcg gggccggccg ggctcgtcgc cggctccgtg
     4501 ggcggcggcg ggggcgaggc ggtggtcctc gacgcggccc gcgaggcgct cggcgacggg
     4561 aaggcgcgcg tcctcgaggt cgagaagctc gggaacgcag ccgaggggcc ggagatggtc
     4621 tgcggaggga cggccctgat cctcgtcgag ccgctcgggt cgaagtccgc gtacgccgcc
     4681 gcccgggagc tcctctcccg cggcgagcgg gcccttctcg cgcgccggac cgcgaccggc
     4741 gagagcgccg cgatgggccg ctcgggcgtc tggtccccag cccccccggc gggcgccgac
     4801 cccgaggcgg cgcgccgcgc cctcgagcac ggcctcccgc tcctcgacga ggcggccggc
     4861 gtcttctacg acccgctcgt cccccccgag aggctcctcg tcctcggcgg cgggcacgtc
     4921 ggccgcgcgg ttgccgccgc agcgcacgcc gtcgggttcg ccgtcaccgt ggcggacgac
     4981 cgcgccgagt tcctggcgcc ggagcgctac cccgaaggcg tgcagaccca ccacggctcg
     5041 tacaccgggg ccgtcggcgc gttcccctgg gacgcctcgg cctacgccgt catcgtcacg
     5101 cgcggccacc tcgccgacct cgaatcgctc cgggccgtcc tcggcaagcc gtggcgctac
     5161 gccgggatga tcgggagccg gaagaaggtc cgactcatgc tcgatcaggc gatcgcggac
     5221 ggattcgacc cggagagggt cgccgcggtg aacgcgccga tcggcctcga catcggcgcg
     5281 gagacgccgg aggagctggc cgtgtcgatc gtcggcgagc tcgtcgccgc gaggagaggg
     5341 gtcgccgcgg cgagcctcgc gaggaggcgg ccacgaagcg cgtcctgaag ctcgccgacc
     5401 tcctcctgcc gctcctgccg gagggccgag gcatcgtgtc gttcgtcggc gcgggcggca
     5461 agacgagcct cctcctccac ctcgcccgcg agctcgccgc cgacagccgc cccgtcctcg
     5521 tcacgacgac gacccatctc gccgacccgc gcgaggagcc gggagcgttc gtcgaacggg
     5581 tccttctcct gcccgccctg gagcaccccg accccgacgc gccactcccg gcgcccacgc
     5641 ccggagtgac cctcctcgtc tcccgtacgg ccgagccgcc ccgcaagctc aaggggatcg
     5701 acccgtctca cgtctcccgc ctcgcatccg tctggcccgt cgtcctcgtc gaggccgacg
     5761 gctcgaaacg gctcccgatc aagtcccctg cgggacacga accggtcgtc ccggccgaga
     5821 ccggactcac catcggcgtc ctcggtctcg acgcgctcgg ctggccgatg gacgaacgga
     5881 cggtccaccg cccggagcgc ttcgcggccg tcaccggctg ccttcccggc gcggcgatcg
     5941 gctgggacca cgtcgtcgcc ctcgcgcgcc atcccgaggg gctcttcaag ggggcgcgcg
     6001 gcgcccgcgt cgccctcctc aacaaagtcg acgagtcgcc ctacctcccc tcccctggcc
     6061 agctccgcgc cctcggcgcc gacctcgtcc tcctgagcgg cgcggacgag gcggagcgcg
     6121 ccttcttcac ggtcccgcag gggcccgcca cgtgagcctg gcgggcggac gcctcgtcgt
     6181 cctccgcggc gcaggcgacc tcgccaccgg ctgcatcgtc cggctcgtcc gctccggatt
     6241 ccgggtcgtc gcgctcgagc tcccccggcc gagcgccatc cggcgcaccg tgagcttctc
     6301 ggaagccgtc tacgacggga gcgccgaggt ggaaggaatc cgcgccgtcc gccacgacga
     6361 gccacccgcg gcgtgggacg ccgccacggt cccgatcctc gtcgacccgg aggcgcgcct
     6421 cgtgccccgc ctcgcccccc tcgccgtcgt cgacgcgatc ctcgcgaaga ggaacctcgg
     6481 aacacggatc gacctcgccc cggtcgtcgt cgcgctcggc cccggcttcg aggcgggccg
     6541 ggacgcgcac gccgtcgtcg agacgaaccg cgggcacgcc ctcggccgcg ttctcctcgc
     6601 gggacgcgcc gaggacgaca cggccgtgcc gggcacgatc gcggggcatg ccggcgaccg
     6661 cgtcgtccgg gcccccgccg ccggagaggt cgtggcgctc cgcgcgatcg gggacctcgt
     6721 ggccgccggc gagccggtcc tcaccgtcgg gggcgccgtc gcccgcgcgt cgatcggcgg
     6781 cgtcctgcgc gggctgattc gtccgggcta cccggccccc gccggcctga aagtcgccga
     6841 cgtcgacccc cgcggcgtgc gggagcactg cttcaccgtt tccgacaagg cgcgggccgt
     6901 ggccggcggc gtcatcgagg cgctcctcgc cctcgctccc ggctgaggcg ggaggccggc
     6961 cctcagtccg ccttcggctt cgcgggccgc tccacgcgga tcgcgccggg ctgcatcggg
     7021 agccagtgct cgacgaagag cggctgcgcc acctcgtccg ccgccttcag ccgattcgac
     7081 gtgccggccg tgtccttcgc cgcgacggcg atcgcgaggg cgtgagagac gacggagaac
     7141 gccgagagtc cgaagctgaa caggaagtct tcggtcggca ggacgatcgc gaccgtcgcg
     7201 ctcggcgtca gcggcgagtc gtggcggtcg gtgaacgcca ggaccgggat ctcgcagcgc
     7261 cgggcgaaag ctgccgcgtc gaccgtctcg cggctgaacg gggcgcagct gacgacgacg
     7321 aggaggtccc cggcgcccag gttcgccacc tggttcgaga agtccgacgg gctcgcggcg
     7381 atgcacggca gccccgcctg cgtcaggttg aacgcgagca cctggcacat caggttcgag
     7441 acgccgcggc cgagaaggac gcggtggcgg gcccgcgtca ggagctgcgt cgcggagtcg
     7501 agagtcgggt ggtcgagcgt ctgaacgagc ctcgcgaggt tctcgcggtc gcgccgcacg
     7561 acctcgccga gggcccagaa cgggtccgcg ggcgcctcca gcgcctccag cggatcgccc
     7621 cccgagcgaa tctggcacgc ctcccggagg gcgtcccgga actcgctgaa gccgcggtac
     7681 ccgagcctct tcgagaagcg gacgaccgtc ccgatgctga cgttcgcccg ctccgccagc
     7741 gactcgatcc cccagagcgc gccgaggaga gggtcggaca tcagggcgtc ggcgatccgc
     7801 cgcatcgcct ccgggaggtc gtggtagacc tccttcaggc gctcccggat ctcctgcggc
     7861 tctcccgacg cgaccattcg cgcctcgatt ctagcccgtg ctccgaagcc ccgccgcccg
     7921 cccggcccct cgggaccggg cgggcgcggg ctccccctca cggaagcgga acgtcgcgga
     7981 acgcgaacgt gtgacggacg aggctcgagt cggagcgcat ctggacgctc agcgtccagt
     8041 cggcctgcgg aggctcgttc cagaaggtca ggacgcggaa gcccgactcc tccgagtcgg
     8101 tcccgaagac gcgctcgccg ttcgcgccga acgccttgat ctcctggatc gccttcttcg
     8161 ggtccttgac cttcaggatc acctcgcgct cctccccctt cgggaaggag tagagcgcgc
     8221 tctcgaccat cgaccggatc gagtcggcgt cctcgtagcc ctccttcttc agcttcgcgg
     8281 tctcggcctc ctcggccttc ttccgctcgg cgtcgagctg ggccttcgag acgaacgaga
     8341 tctcgacgcc gttcgcgcgg agcgcggggt gcacggccgc cttccccgcg agcgcgagga
     8401 acctctcgat ccgcgcctct ccgttcgggt cgcgcgacgg gacgaagagc tcgatctctc
     8461 ccgagacctc cttcagcgcc ttcgccgccc gcggcgggtt cttcatctcg gcgaagacga
     8521 tcgtcggcgg cgcggccttg ccgccctcgg gcttcccgaa ctggccgcgc tgcgtcggct
     8581 cgagcgggac ctcggcggcg tcctccttga cgagatcggt gccgaggtcg tcgacggcct
     8641 tcctcacgac cacccgggcg gccgtcacct cgtgtgcggg atagtccggg agctccatcc
     8701 cgagcgtgca gcgcgggaag tgcccgttcg accggcggtc ctggagggcc gtcatccggg
     8761 cgttcgcggg cgtgatcttc ggcgccgccg cgggcttcgt gaccgccggc ttcttcttct
     8821 tcgactgagc ctcggcggga gcggccgcga gggccgcgcc gaggaggaga gcgagggaga
     8881 cgtgcgtcgc gttcacggga gccggacctc cttcatctcg aacgggaccg agacgatcga
     8941 cttgtcggtc aggagccgcg ccacgaggac cgcgtccttc ggcggggccg ccttcagcgc
     9001 cacctgcttc aggacgaacg cctcgtcgga gcgcgaggac gagcctccgc tctcgagctt
     9061 ctcccccttc gccgtctcca gccagatctc ctggatcttc tcctcgctcg ccgccgggac
     9121 cttcaggtag aaggcgtgct cggggaggtc cccctccccc atctcgtcga acgccttcgc
     9181 catctcgacg agcgcgtcgg tctcctcgtc cttcatcccg cgcgccttcg cctcctcgcg
     9241 gatcttcgcg atcttcgcct cgtcgagccg gtgcttcttc cgctcctcga cgtacttcgc
     9301 cctcgacagg agcgtcagct ccaccttcgc cgacttcagc cccttcgaca cgaccggttt
     9361 gtcgagcttc gcgagcgctc ccggcatcct caccacggcc gaggcgtcgc gcccggggac
     9421 gaagagctcg acggttcccg ccacccggac ggtgctcgcc ccgcgcggcg ggctgttcac
     9481 gacgacctgc agcgtcccgg cgttcacgtt ccggtcggag aagtcgggcg ccttctcctc
     9541 cttcggcagg aggtcctcgc ccgtgtcggc cttcgcgctc ttgagccgca cccgcgccga
     9601 ctgcacgacg tcgaggccgt cgccttcgag gtcgagctgg aggacgaggc cgcctttcat
     9661 gagcccctct gagatccggt cgtcgacgac ctccttcgcc acgaccttca cgttcgtctg
     9721 agcctcggtc gtccccgccg cgccgagggc gagaaacgcc ccgagcgcga cggcgcggcg
     9781 cactgcggat cgcaccatcg ttcacctcca gctgtctggt tgcagcgaga ctaacccggt
     9841 ttcgcgccct tcgccggggg cgtgatccgg gtcacgtcgg ccctccgcgg acgctcgcgg
     9901 taacatccgg cccggcgggc cgttagctca gcggttagag catctgcctt ttaagcaggg
     9961 ggtcccgggt tcgaatcccg gacggctcac catctccgcc acgtcgaggc accggcgaac
    10021 gtcggcggcg aacgcgcacg tcgaggtccc ggacggcgcc ccatggtaaa ttccgcccgg
    10081 cgccgcggtg cgctccagcc tggtcgcccc catcgtctag tggcctagga cgtcaccctt
    10141 tcaaggtgaa gatcgcgggt tcgaatcccg ctgggggtac ca
//
LOCUS       DAOTEZ010000112        10156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4660_length_10156_cov_6.020097, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000112 DAOTEZ010000000
VERSION     DAOTEZ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10156
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4660_length_10156_cov_6.020097"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1631
                     /locus_tag="P5164_07695"
     CDS             <1..1631
                     /locus_tag="P5164_07695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRY43813.1"
                     /translation="SAALLAALLAPGTDGAEEPQTLPRVRGAVELSLVNVDVAVTGKD
                     GRPIEGLAASDFAVVHGKSPVSISNFREERLVPRAEVSMAVPVPAPVPIASEAGAAVL
                     EAPPARRHVILFVDHLALPDKREREQVFGSLKELVKKTVRPGDGVMVVSWRRGVRMAW
                     PYTDDVARIERALDLVAGGTIRLDRDAQNDLDQFAATEAFQAWAGEPPDTSLSRDLSV
                     EQAYNELKGKASAVMGLVSTMAGLEGKKVLVLASRSFSRRPGVEYSGHRVETGRLLDE
                     VAARANAAGVTIHALYAAAWQPDLPNVTDSRFQPPGAAGSRSATGRSDARLEDLAGLE
                     TLSGRTGGVLVGTTMEAPLFAERVSNDLVHWYSIGYPVPPGASASARISVRVNRKGAK
                     VRTRREVIDRTPEQRMEDRVLANLFRADLSGRLPIGLSVEPARREKERFVRTVTLRVP
                     VKDLVLLPEAAARRGAVNVFWVVAEPNGEFSGVRSERHEVAFPPEESAPGPETELRLE
                     VPIETSGPEARISLGVRDEVGGEAGFGLIRPGSR"
     gene            1648..2535
                     /locus_tag="P5164_07700"
     CDS             1648..2535
                     /locus_tag="P5164_07700"
                     /inference="COORDINATES: protein motif:HMM:NF024484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HRY43814.1"
                     /translation="MKPERGTWRRAGKAARRAARLAGFGLLAAAGPGLHADDAVPGAG
                     ARESATFAEACPLFREGASGSFGFGEETFGGGKAAWTMEPAGGFSLCPEVAGASVEER
                     PAPRWVPWTERDPREPLRGVRNATAPHRKRLVRSSVILTGTAIASGAIDSLLPKEETN
                     WEEGAFDHGLSNLGRAWSTLPVWDRDGAFHDWFGHPYAGAFYYNMMRSQGATVSQSFL
                     YCVAQSVLWEYVLEAAAEQPSIQDLVSTPIMGAALGEALHRWSISILRKGRLNFGEKV
                     LVFLLNPSYVINNGYRAPE"
     gene            complement(2564..3184)
                     /locus_tag="P5164_07705"
     CDS             complement(2564..3184)
                     /locus_tag="P5164_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43815.1"
                     /translation="MRRLFALLALALAAAPPLRAQEVRIVELTPFLGARSGGRLKDFG
                     TGEWFSVRPSWSWGFVAGIAPGRPNLIFEGTFLRQATRLDAENAFLGGDGNLHDLDLE
                     TGLAGVQWDFAPKARVRPFVSGAVGATSFEAQGGGYTTSFTASLSGGVKVMTTRGFGL
                     KVQMRALGFFSGSSRRDLCRWSSCEVAVPSNGTYQVDLSAGTFFAF"
     gene            complement(3229..4299)
                     /locus_tag="P5164_07710"
     CDS             complement(3229..4299)
                     /locus_tag="P5164_07710"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HRY43816.1"
                     /translation="METLTLLGTTLGLSFTAGLNLYATILVTGLAVRFDWLTLPAGLE
                     GLAVLSHPAVLAAAGLLYVVEFLADKIPAVDTVWDVLHTFVRPLGAVLISWAAVSDAN
                     VPKPLEIPLLLLAGGVSLSTHAGKAGTRLASTATGGHATGVGVGLSLLEDVAAVSIAP
                     LALAYPVVTLVLVVVALALLALVAPLGWRLLRSRVAALLYLFQHKLVDRELPGLDTSH
                     LPEDVALAFADAGVRSGGDGLLLPAYASSLPGVPRWIPGYVALVPGGAVFACRGWFRT
                     RVGRLSFSDGAPLLVRRLTGYVLHARTPKRRATITFFPLTKPMGQALEAAVAREASAT
                     SAGPLPSAPPPVVAASASAAPL"
     gene            4470..5063
                     /locus_tag="P5164_07715"
     CDS             4470..5063
                     /locus_tag="P5164_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY43817.1"
                     /translation="MKTRRPVRPYRMVARAEAAAATHARIVEAALLRLSERPFDEVSL
                     ADVARDAGVTVQTVLRRFGSKEGLAEAATELGLERVRAQRWSAPPGDVGGAVRALLAH
                     YEEWGERSLRFLAQEERVPAMRRVVAVARALHHEWVDHVFAGRLARERGAARARLRAR
                     LVAVTDVCTWKVLRRDLGLDARAAGATFRELVEAVAR"
     gene            5076..6308
                     /locus_tag="P5164_07720"
     CDS             5076..6308
                     /locus_tag="P5164_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY43818.1"
                     /translation="MARVLVYTSPARGHLYPLVPTLEELRRRGHGVAVRTLASEVGRL
                     GALGLSAGPINPAIEELEHEDWKAASAPAALLAACRTFVARGAHEVADLRRAIAAERP
                     EVLFTDVNSWGAAAAAEASGIPWAAFAPYFLPLRSPGVPPWGLGLAPARGAAGRLRDA
                     LLWPVVDRLYDRVRPSLNELRASAGAPRLRHVSDFATRAPLVVSYTAEPFEYPRAWPA
                     NVKLVGPGVWEPGEEAEPSRAGDDRPLVLVTCSTEFQNDGRLVAAALAALADEPVRVV
                     ATTASLDPASFEAPANARVERFLPHGPLLCEAACVVCHGGMGITQKALAAGVPVCAVP
                     FGRDQLEVARHVEVAGAGTRLPAARLNPERLKAAVRTAMSRAEGARRLAAAFREAGGA
                     RAAADALESIVTERRARP"
     gene            6335..8401
                     /locus_tag="P5164_07725"
     CDS             6335..8401
                     /locus_tag="P5164_07725"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRY43819.1"
                     /translation="MKTARAPFALALLTLLAATAALAEAPAAKAPLGLADLFRVRAAR
                     EVRISQDGKAVAFLVQVPRTPGKDEDGPAWSELHVVERGKAARPFVAGPENASRISFS
                     RDGAEVFFVAKRKGDPTAALWSIRRDGGEARRRVTLPADVEGYTLSPKEDGLVALLAK
                     EKKAEAVEAREKKGFDREVFEEEWRPVKVWVARLDGEAPSRALDLPGSASDLLFAPTG
                     DRLAVALAPTPSVDDGYMNRKVHVVALDGKVVGRVENDGKLGSVAWSPDGTRLAMICA
                     ADRNDPSTAALGVVPATGGRPKDLLAGFDGHPVSFDWAGNDRLVVVAARGVATELLEL
                     PAVGGAPKRLPLPEGLVVDGLDRAADGTLAFVAERPDRPAEPFFLAAGTSKPEPLADL
                     NPWLSERRLGKQSVVRWKARDGVELEGILVEPLDRPAGTRVPLVLLVHGGPEAHVSNG
                     WLTRYSEPGQLLAAKGIAALYPNYRGSTGRGLAFSKTSQGDPAGKEFDDLVDAVDHLV
                     ATGLADPKKVGITGGSYGGYAANWAATRLTERFAAAVSFVGITDLTSKAGTTDIPIED
                     VDVHMLTMPWTRFEQSRERSPLSYVAKARTPLLLLHGTADPRVHPTQSLMLYRYLKLA
                     GHPALRLVRYPGEGHGNRRAASQLDAAVRILGWFEHYLLGAGGAPPAPEAADGAVLEV
                     VEAAKK"
     gene            8517..9818
                     /gene="glgC"
                     /locus_tag="P5164_07730"
     CDS             8517..9818
                     /gene="glgC"
                     /locus_tag="P5164_07730"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HRY43820.1"
                     /translation="MEETLAFVMAGGQGERLRPLTDVRAKPAVPFGGIFRIIDFTLSN
                     CVNSGLRRIYVLTQYKSYSLSNHLRTGWNMLSPRLNQFIDEVPAQQQLGSSWYQGTAD
                     AIRQNFSFVESKRPRLVLVLSGDHVYKMDYRLLRAFHDEKGAELTVACVRLPAEEARG
                     TFGVMQVDEEGRIVGFEEKPAEPKTIPGTTDCLASMGIYLFEAGCLRRGLDNDLLDFG
                     KDVIPLLLREGVPVYAYDFTTRNAIPEWEHTKREGVRRKELVPRASDADYWRDVGTLE
                     QYWLANLDLVQPKPRFNVYGERFPLYSAPGHFPPAKFVHEMPGRTGVAVNSMVADGVI
                     VSGGTVRESVLGSGVYVHSWAMIEKSVLLGGALRGGVLTETIIGRRCRVKNAIIDKNV
                     RLSENVSIGYDREDDERRGLRTVPITGSSEHIVVVPKDFSL"
     gene            9886..>10156
                     /locus_tag="P5164_07735"
     CDS             9886..>10156
                     /locus_tag="P5164_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43821.1"
                     /translation="MAWGRSRKSSGRERAAGPAERMVRVGCPAHNCGGRCVLLATVRD
                     GRIVRLEADDRPDVLEAPQLRACARGRSYLRRQYHPDRLLHPLKRV"
BASE COUNT         1386 a         3538 c         3966 g         1266 t
ORIGIN      
        1 cttccgccgc gcttctcgcc gcgctcctcg cccccggaac ggacggggca gaggagccgc
       61 agacgctgcc gcgcgtgcgg ggcgccgtcg agctctcgct cgtcaacgtc gacgtggcgg
      121 tgacgggaaa ggacgggcgg ccgatcgagg ggctcgcggc gtccgacttc gccgtcgtcc
      181 acgggaagtc gccggtctcg atctcgaact tccgcgagga gcgtctcgtg ccgcgggccg
      241 aagtctcgat ggccgtgccc gtgcccgcgc ccgtcccgat cgcgtcggag gccggggcgg
      301 cggttctcga agcgcctccg gcccgccggc acgtcatcct cttcgtcgac cacctcgcgc
      361 tgcccgacaa gcgggagcgc gagcaggtct tcggttcgct gaaggagctc gtgaagaaga
      421 cggtccggcc cggcgacggc gtgatggtcg tctcctggcg gcgcggggtc cggatggcct
      481 ggccctacac cgacgacgtg gcgcggatcg agcgggcgct cgacctcgtg gccgggggga
      541 cgatccggct ggaccgcgat gcgcagaacg acctcgacca gttcgcggcg acggaggcgt
      601 tccaggcctg ggccggcgag ccgcccgaca cgtcgctctc gcgggacctg agcgtcgagc
      661 aggcgtacaa cgagctgaag gggaaggcgt cggcggtgat gggcctcgtc tcgacgatgg
      721 cggggctgga ggggaagaag gtcctcgtcc tcgcctcgcg gagcttctcc cgacggccgg
      781 gcgtggagta ctcggggcac cgcgtcgaga cggggcggct cctcgacgag gtggccgcgc
      841 gcgcgaacgc ggcgggggtg acgatccacg cgctctacgc ggcggcgtgg cagcccgacc
      901 tgccgaacgt caccgactcg cgcttccagc cgccgggggc cgcgggctcg cgctccgcga
      961 ccggacggag cgacgcccgc ctcgaggacc tcgccgggct ggagacgctc tcgggccgga
     1021 cgggaggcgt cctcgtcggg acgacgatgg aggccccgct cttcgccgag agagtctcga
     1081 acgacctcgt ccactggtac tcgatcggct accccgttcc tcccggcgcg agcgcctcgg
     1141 cgcgcatctc ggtccgcgtg aaccggaagg gggcgaaggt gaggacgcgg cgcgaggtga
     1201 tcgaccggac gccggagcag cggatggagg accgggtcct cgcgaacctc ttccgggccg
     1261 acctttcggg gcggctcccg atcggcctct cggtggagcc cgcgcgccgc gagaaggagc
     1321 gtttcgtccg gacggtgacg ctccgcgtgc cggtgaagga cctcgtcctg ctgccggagg
     1381 ccgccgcgcg gcgcggcgcg gtgaacgtct tctgggtcgt ggccgagccg aacggcgagt
     1441 tctccggcgt gcggagcgag cgccacgagg tggcgttccc cccggaggag agcgcgcccg
     1501 gcccggagac ggagctgcgg ctcgaggtcc cgatcgagac gagcggcccg gaggcgcgga
     1561 tctcgctcgg cgtccgagac gaggtcgggg gcgaggccgg cttcggcctg atccggcccg
     1621 gcagccgctg agggagacgg ggcggccatg aaaccggagc gggggacgtg gagaagggcc
     1681 gggaaggcgg cgcgccgcgc cgcgcggctc gcgggcttcg ggctgctggc ggcggccggg
     1741 ccggggctcc acgcagacga cgccgtgccc ggagcgggag ctcgcgagtc cgcgacgttt
     1801 gcggaggcct gtccgctctt ccgggagggc gcatccggct cgttcggttt cggcgaggag
     1861 acgttcgggg gcgggaaagc cgcgtggacg atggagcctg ccggcggctt ctctctctgc
     1921 ccggaggtcg cgggggcgag cgtcgaggaa cggcccgcgc cgaggtgggt gccgtggacg
     1981 gagcgggacc cgcgggagcc gctccgcggc gtgaggaacg cgacggcgcc ccaccggaag
     2041 aggctcgtgc gcagctcggt gatcctgacc gggacggcga tcgcgagcgg ggcgatcgac
     2101 tcgctcctgc cgaaggagga gacgaactgg gaggaggggg ccttcgacca cggcctctcg
     2161 aacctcgggc gcgcgtggtc gacgctgccc gtctgggacc gcgacggggc cttccacgac
     2221 tggttcgggc acccgtacgc gggcgccttc tactacaaca tgatgcggtc gcagggggcg
     2281 acggtcagcc agtcgttcct gtactgcgta gcccagtcgg tcctctggga gtacgtcctc
     2341 gaggccgccg ccgaacagcc gagcatccag gacctcgtct cgacgccgat catgggcgcg
     2401 gcgctcgggg aggcgctcca ccgctggtcg atctcgatcc tgaggaaggg gcggctgaat
     2461 ttcggtgaga aggtgctcgt cttcctcctg aacccgtctt acgtgatcaa caacggctac
     2521 cgcgccccgg agtgaggtgc ggcggcggcg gcgcgccgcc cgctcagaac gcgaagaacg
     2581 tcccggcgga gaggtccacc tggtaggtgc cgttcgacgg gacggcgact tcgcagctcg
     2641 accagcggca gaggtcccgt cgcgagctcc ccgagaagaa gccgagggcc cgcatctgga
     2701 ccttcaggcc gaagcctcgg gtcgtcatca ccttgacgcc gccggagagc gaggccgtga
     2761 aggaggtcgt gtagcccccg ccctgggcct cgaacgaggt ggcgccgacg gcgccggaga
     2821 cgaacgggcg gacgcgcgcc ttcggggcga agtcccactg cacgccggcg agccccgtct
     2881 cgaggtccag gtcgtgcagg ttgccgtcgc ccccgaggaa ggcgttctcg gcgtcgagcc
     2941 tcgtcgcctg ccggaggaag gtcccctcga agatgaggtt cgggcggccg ggcgcgatcc
     3001 ccgccacgaa gccccacgac cacgacggcc ggacggagaa ccactcgccg gtcccgaagt
     3061 ccttcaggcg gccgccggag cgcgcgccga ggaacggcgt cagctcgacg atccggacct
     3121 cctgggcgcg gaggggaggg gccgcggcga gcgcgagcgc gaggagggcg aagagccgac
     3181 gcaccggcgg attctgcccg cccgcgggct caggagcgag ggggctgctc agagcggggc
     3241 ggcgctcgcg ctcgcggcga cgacgggcgg gggcgccgag gggagcgggc cggcggacgt
     3301 tgccgacgcc tcccgcgcca ccgcggcctc gagcgcctgg cccatcggct tcgtgagcgg
     3361 gaagaaggtg atcgtcgcgc ggcgcttcgg cgtccgggcg tggaggacgt agccggtgag
     3421 gcgccgaacg aggagcggcg ccccgtcgga gaaggagagg cgaccgacgc gcgtccggaa
     3481 ccagccgcgg caggcgaaga cggctccgcc ggggacgagg gcgacgtacc cggggatcca
     3541 ccgcgggacg cccgggagcg aggaggcgta ggccggaagg aggaggccgt cgccccccga
     3601 gcggacgccc gcgtcggcga aggcgagggc cacgtcctcg gggaggtggg aggtgtcgag
     3661 gccgggaagc tcgcggtcga cgagcttgtg ctggaagagg tagaggagcg ccgccacgcg
     3721 ggagcggagg agcctccagc cgagcggggc gacgagcgcg aggagggcga gcgcgacgac
     3781 gacgaggacg agcgtgacga cggggtaggc gagggcgagc ggcgcgatcg agaccgcggc
     3841 gacgtcctcg aggaggctga ggccgacgcc gacgccggtc gcgtggccgc cggtggccgt
     3901 cgacgcgagg cgcgttccgg ccttgccggc gtgggtcgag agggagacgc ccccggcgag
     3961 gaggaggaga gggatctcga gcggcttcgg cacgttcgcg tcggagacgg cggcccacga
     4021 gatgaggacg gcgccgagcg gccggacgaa cgtgtggagg acgtcccaga cggtgtcgac
     4081 cgcggggatc ttgtcggcca ggaactcgac gacgtacagc agcccggcgg cggcgaggac
     4141 ggccgggtgg gagaggacgg cgagcccctc gaggccggcc gggagcgtca gccagtcgaa
     4201 gcggaccgcg aggcccgtga cgaggatcgt cgcgtagagg ttgaggccgg cggtgaagct
     4261 gaggccgagc gtcgtcccga ggagggtcag ggtctccatc gcggctcctt cccgggccgg
     4321 gctctcgcgg ccggcccggg ggaatggtag cgccgcgcgg ggcgcccggg ggccacgggc
     4381 cggaccgccg ccggggcgcg gccgacggcc tccctatttc atgtcaggcg aattgacatg
     4441 gaaaacgcat cggaattagc atcagtgaca tgaaaacgcg caggccggtc cgcccctacc
     4501 gaatggtcgc gcgcgccgag gcggccgcgg cgacccacgc acgcatcgtg gaggcggcgc
     4561 tcctccggct ctcggagagg cccttcgacg aggtctcgct cgccgacgtc gcgcgcgacg
     4621 cgggcgtgac cgtccagacg gtcctgcggc gcttcggctc gaaggaaggg ctcgcggagg
     4681 cggcgacgga gctcggcctc gagcgggtgc gggcccagcg ctggagcgcg ccgcccggag
     4741 acgtcggcgg tgcggtccgg gcgctcctcg cgcactacga ggagtggggg gagcggagcc
     4801 tccgcttcct ggcccaggag gagcgcgtcc ccgccatgag gcgggtcgtc gccgtcgccc
     4861 gcgcgctcca ccacgagtgg gtcgaccatg tcttcgccgg ccggctcgcg cgcgagcggg
     4921 gcgcggcgcg ggcgcggctc cgcgcgcggc tcgtcgccgt cacggacgtc tgcacgtgga
     4981 aggtcctccg gcgcgacctc gggctcgacg cccgggccgc cggggcgacc ttccgcgagc
     5041 tcgtcgaagc cgtcgcgcgc tgagaggagg agcccatggc ccgagtcctc gtctacacct
     5101 cgcccgcccg cggccacctc tacccgctcg tcccgacgct cgaggagctc cggcggcgcg
     5161 ggcacggcgt cgcggtccgg acgctggcct cggaggtcgg ccggctcggc gcgctcggac
     5221 tctcggccgg gccgatcaac ccggcgatcg aggagctgga gcacgaggac tggaaggcgg
     5281 cctcggctcc ggcggcgctc ctcgcggcgt gccggacgtt cgtcgcgcgc ggggcgcacg
     5341 aggtcgccga cctgcggcgg gcgatcgcgg cggagcgccc cgaggtccta ttcaccgacg
     5401 tcaactcctg gggcgcggcg gcggccgccg aggcctccgg cattccgtgg gccgcgttcg
     5461 cgccctactt cctgccgctg cgctcgcccg gcgtgccccc gtggggcctc gggctcgcgc
     5521 cggcccgcgg ggcggccggg cggctgcgcg acgcgctcct ctggcccgtg gtcgaccggc
     5581 tctacgaccg cgtgcggccg tccctgaacg agctccgggc ctcggcgggc gcgccgcggc
     5641 tccgccacgt ctccgacttc gcgacgcgcg cgccgctcgt cgtctcgtac accgccgagc
     5701 cgttcgagta cccgcgggcc tggcccgcga acgtgaagct cgtcggcccc ggggtctggg
     5761 agccggggga agaggcggag ccgtcgcgcg ccggagacga ccgcccgctc gtcctcgtca
     5821 cctgctcgac ggagttccag aacgacggcc ggctggtggc cgcggcgctg gccgcgctcg
     5881 ccgacgagcc ggtccgcgtc gtcgcgacga cggcgtccct cgacccggcg tccttcgaag
     5941 cgcccgcgaa cgcgcgggtc gagcgattcc tcccgcacgg cccgctcctc tgcgaggcgg
     6001 cctgcgtcgt ctgccacggc gggatgggga tcacgcagaa ggcgctcgcg gcgggcgtcc
     6061 cggtctgcgc ggtgccgttc ggccgggacc agctcgaggt ggcgcggcac gtggaggtcg
     6121 ccggggccgg gacgcgcctg ccggcggcgc gcctgaatcc ggagcgactg aaggcggcgg
     6181 tccggacggc gatgtcgcgg gccgaagggg cgcgccgcct cgccgcggcg ttccgggagg
     6241 cggggggcgc gcgggccgcc gccgacgccc tcgagtcgat cgttacggag cgacgcgcgc
     6301 gcccctagaa tcgcgggatg cacggaggga accgatgaaa acggcccgcg ccccgttcgc
     6361 gctcgcgctc ctgacgctcc tcgccgccac ggcggcgctc gcggaggccc ctgcggcgaa
     6421 ggctccgctc gggctcgccg acctcttccg cgtccgcgcc gcccgtgagg tccggatctc
     6481 gcaggacggg aaggcggtcg ccttcctcgt ccaggtcccg aggacgccgg ggaaggacga
     6541 ggacgggccg gcgtggtcgg agctgcacgt cgtcgagcgg gggaaggccg cgcgtccctt
     6601 cgtcgcgggc ccggagaacg cctcgaggat ctccttctcg cgcgacggcg ccgaggtctt
     6661 cttcgtggcg aagcggaagg gggacccgac cgccgcgctc tggtcgatcc ggagggacgg
     6721 cggcgaggcg cggcgccggg tgacgctccc ggccgacgtg gaggggtaca ccctgtcgcc
     6781 gaaggaggat gggctcgtcg ccctcctcgc gaaggagaag aaggcggagg cggtcgaggc
     6841 gcgcgagaag aagggcttcg accgggaggt cttcgaggag gagtggcgcc cggtaaaggt
     6901 ctgggtcgcg cgtctcgacg gagaggcgcc gtctcgcgcg ctcgacctgc cgggctcggc
     6961 gtccgacctc ctcttcgcgc cgacgggcga ccgcctcgcg gtggcgctcg cgccgacgcc
     7021 gtcggtggac gacggctaca tgaaccggaa ggtccacgtc gtcgcgctcg acgggaaggt
     7081 cgtcgggcgg gtggagaacg acggcaagct cgggagcgtc gcctggagcc ccgacgggac
     7141 gcgcctcgcg atgatctgcg cggccgaccg gaacgacccg tcgacggccg ctctcggcgt
     7201 cgtcccggcc acgggcggcc ggccgaagga cctcctcgcc ggcttcgacg gccacccggt
     7261 ctccttcgac tgggcgggca acgaccggct cgtcgtcgtc gcggcgcggg gcgtcgcgac
     7321 cgagctgctc gagctccccg ccgtcggcgg ggcgccgaag cggcttccgc tgccggaggg
     7381 gctcgtcgtc gacggcctcg accgggcggc ggacggcacg ctcgcgttcg tggccgagcg
     7441 gccggaccgt cccgcggagc ccttcttcct cgcggcggga acgtcgaagc cggagccgct
     7501 cgccgacctc aacccgtggc tctcggagcg acggctcggg aagcagagcg tcgtccggtg
     7561 gaaggcgcgg gacggggtcg agctcgaggg gatcctcgtc gagccgctcg accggcctgc
     7621 cggaacccgc gtgccgctcg tcctcctcgt ccacggcggc ccggaggcgc acgtctcgaa
     7681 cggatggctg acgcgctaca gcgagccggg ccagctgctg gccgcgaagg ggatcgccgc
     7741 gctctacccg aactatcgcg gctccaccgg gcgggggctc gcgttctcga agacgagcca
     7801 gggggacccg gcggggaagg agttcgacga cctcgtcgac gccgtcgacc acctcgtcgc
     7861 gaccggcctc gccgacccga agaaggtggg gatcaccggc ggctcctacg gcggctacgc
     7921 cgcgaactgg gccgccacgc ggctcaccga gcggttcgcg gccgccgtgt cgttcgtcgg
     7981 gatcaccgac ctgacctcga aggcgggaac gacggacatc ccgatcgagg acgtcgacgt
     8041 ccacatgctc acgatgccct ggacccgctt cgagcagagc cgggagcgga gccccctctc
     8101 atacgtcgcg aaggcccgga cgccgctcct cctcctccac gggacggccg acccgcgggt
     8161 ccacccgacg cagtcgctga tgctctaccg ttacctgaag ctcgcggggc acccggcgct
     8221 ccgcctcgtc cgctacccgg gcgaggggca cgggaaccgg cgggccgcct cgcagctcga
     8281 cgcggccgtt cggatcctgg ggtggttcga gcactacctc ctcggcgcgg gaggcgctcc
     8341 tccggcgccg gaggccgcgg acggagccgt cctcgaggtc gtcgaggcgg cgaagaagta
     8401 gacgacggcc ggccgatccc agcgacgcgg ctcgacgcgg gaatcggccg gtttctcgca
     8461 cggcgccacg gcccggcgct aagctacccg atgcggcacg cggaaggagg gggacgatgg
     8521 aagagaccct cgcgttcgtc atggcgggcg gacaggggga gcggctccgg ccgctcacgg
     8581 acgttcgggc gaaaccggcg gtccccttcg gcgggatctt ccggatcatc gacttcacgc
     8641 tctcgaactg cgtgaactcg ggcctccggc gcatctacgt cctcacgcaa tacaagtcgt
     8701 actccctttc gaatcacctc aggaccggct ggaacatgct gagcccgcgc ctgaaccagt
     8761 tcatcgacga ggttccggcc cagcagcagc tcgggagcag ctggtaccag gggacggccg
     8821 acgcgatccg gcagaacttc tcgttcgtcg agagcaagcg gccgcggctc gtcctcgtcc
     8881 tctcgggcga ccacgtctac aagatggact accggctcct gcgcgccttc cacgacgaga
     8941 agggggccga gctgacggtg gcctgcgtga ggctccccgc cgaggaggcg cgcgggacgt
     9001 tcggcgtcat gcaggtcgac gaagaagggc ggatcgtcgg gttcgaggag aagccggcgg
     9061 agccgaagac gatccccggg acgaccgact gcctcgcctc gatggggatc tacctcttcg
     9121 aggccgggtg cctccgccgc ggcctcgaca acgacctcct cgacttcggg aaggacgtca
     9181 tcccgctcct gctaagggag ggggtcccgg tctacgccta cgacttcacg acgcgcaacg
     9241 cgatcccgga gtgggagcac acgaagcggg agggggtccg gcggaaggag ctcgtcccgc
     9301 gcgcgagcga cgcggactac tggagggacg tggggacgct cgagcagtac tggctcgcga
     9361 acctcgacct cgtgcagccg aagccgcgct tcaacgtgta cggcgagcgc ttcccgctct
     9421 actcggcgcc gggccacttc ccgccggcga agttcgtcca cgagatgccg gggcggaccg
     9481 gcgtggccgt gaactcgatg gtcgccgacg gcgtcatcgt gagcggcggg acggtccggg
     9541 agtcggtcct cgggagcggc gtctacgtcc acagctgggc gatgatcgag aagtcggtcc
     9601 tcctcggcgg cgcgctccgg ggaggggtcc tcaccgagac gatcatcggc cgccgctgcc
     9661 gggtgaagaa cgcgatcatc gacaagaacg tccgcctctc ggagaacgtc tcgatcggct
     9721 acgaccgcga ggacgacgag cggcgggggc tccggaccgt cccgatcacc ggaagctccg
     9781 agcacatcgt cgtcgtcccg aaggactttt cgctgtagcg gatcggttcc gctctcggcg
     9841 aacggccctc tccgagctac gatggccggc tggaggtcgg cgcggatggc ctggggacgg
     9901 tcccggaagt cgagcggacg ggagagggcg gcggggccgg ccgagaggat ggtccgcgtc
     9961 ggctgcccgg cgcacaactg cggcggccgc tgcgtgcttc tcgcgaccgt tcgcgacggg
    10021 cggatcgtcc gcctcgaggc cgacgaccgg cccgacgtcc tcgaagcgcc gcagctgcgg
    10081 gcctgcgccc gcgggcgctc gtacctccgc cgccagtacc accccgaccg gctcctccac
    10141 ccgctgaagc gggtcg
//
LOCUS       DAOTEZ010000113        10146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4671_length_10146_cov_6.037558, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000113 DAOTEZ010000000
VERSION     DAOTEZ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10146
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4671_length_10146_cov_6.037558"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="P5164_07740"
     CDS             complement(<1..396)
                     /locus_tag="P5164_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRY43822.1"
                     /translation="MKPIYIAAYQQSKFGKLMGATVPEIIRTAAEETCRSVKAEASTV
                     DLATIGAVCNLTLNEQGLLSGLLAMTPGLGAKPIESVENACATGGQAILAVAQKLMLG
                     EGDVGLAVGFEKMRDAEGKMDGKLIGKVLG"
     gene            complement(393..1238)
                     /locus_tag="P5164_07745"
     CDS             complement(393..1238)
                     /locus_tag="P5164_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY43823.1"
                     /translation="MNAPALLARQVDGEGEPLLLLNGGMMSYTAWDAYVPPLGERYRV
                     IRCDLRGMLRSPGDRPEGFPGHAEDLLRLLDHLGVAQCHVAGTSYGAFAGLHLAALAP
                     ERVASLVAMTVADRVSPEMWVEADRMAVACREALAGGSREKVYDLVSAFAFSPEWAAA
                     HADEIASRRALVSLLPDSWFSGLASLLGALEGLDVRPLLPRVACPVLVLLADGDRAMP
                     RPWGESLAAALPRGELAVVAGCGHGLVVENPRETLSRLLAFLARHPLPAEGPAREPER
                     NGGTP"
     gene            complement(1235..2776)
                     /locus_tag="P5164_07750"
     CDS             complement(1235..2776)
                     /locus_tag="P5164_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRY43824.1"
                     /translation="MIHADLLGERARLSPDRCALVFVPTGERFTYRALNARAERVAAF
                     LRDGLGLVKGDRVALLAHNRVEYVELFFAAAKAGLVLVPLGTRLTPHELAFIVRNADV
                     SALVYGGDFAETVAALRAEAEMPRRIALDAPATEGDLPLATGLAALPDVSFERTRCDG
                     EDLHLLLYTSGTTGRPKGVMVPHRMTSWNAWNTALCWGLTEHDVSPVITPLYHAGGLG
                     AFFAPILAAGGTVVLHAGFDAAEIWATVARERCTVVLGVPTIWKLLLEHPSFERADLS
                     SIRWLISGGAPLPLYLIEAYQARGIVFKQGYGLTEVGVNCFAMSVEESITKKGSIGRP
                     LMMTEAKLVDESGSEVPAGEVGELCLRGPHVTKGYWRNPEATAAALDDDGFFRTGDLA
                     RKDADGFFTIAGRRKDMLISGGVNVYPAEIEGELLLHPAVMDAAVVGVPDRTWGEVGV
                     AFVVLRPGASATGAELLAFAEPRLARYKLPKEVVFVGDLPRTAYGKVVKGELRDAYLA
                     GRGAS"
     gene            complement(2773..3714)
                     /locus_tag="P5164_07755"
     CDS             complement(2773..3714)
                     /locus_tag="P5164_07755"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY43825.1"
                     /translation="MNKPVKLLLAAAALLVLAAVAAFLWARMRPLAAYAAAGRRALAK
                     SGLERARVASPAGEQSYYRGGSGPVLVLLHGAGDQASAWSLVAPKLLPGRTLVVPDLA
                     GHGASAPAAGPISVPTLLAGLSAVVDAAAPAGKVTIVGNSLGAWLACLWAKEHPERVE
                     RLVLVNGGPLKHAAEGITLLPKDRAEARAAVEKLRDPGSIRVPDFVLDDVVRQAATGP
                     LARLAATSGEMEPFVLDGKLGGLDVPIDLLWGASDQLLPLSYAERMRAELPRARVTEL
                     PRCGHVPQQECPAAFAAALGKLLDGPLPGPAVAPEAP"
     gene            complement(3742..4734)
                     /locus_tag="P5164_07760"
     CDS             complement(3742..4734)
                     /locus_tag="P5164_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005002232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HRY43826.1"
                     /translation="MARYARIVSTGRFVPPIEVPNSALKPRWPQLPDFVDKMEASSAI
                     RTRFYAPEDWATSDVALPAARQALERAGLSPLDLDLIILGTDSPDYVTPATSVVLQGK
                     LGAKNAGTFDVGCACASFPTGVAAAAGLIATNPALRNVLVVGVYLMHKLADPNDPMVF
                     FYGDGAGAAVLQGGDEPGFLNAAMQAGGEYAKCWGIFAGGTAEPATVENVAAGKTTVR
                     MVERYPPEINHEGWPRLVRRLAHEGGFALSDVDQVIFTQVRKPSIELVMNDLGLPVEK
                     AHTIMEKWGYTGSACIPMALDDALEQGKVKKGDLVVMIGSGVGYNQAGVAIRMT"
     gene            complement(4739..5521)
                     /gene="fabG"
                     /locus_tag="P5164_07765"
     CDS             complement(4739..5521)
                     /gene="fabG"
                     /locus_tag="P5164_07765"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HRY43827.1"
                     /translation="MSIETGLAGRAVVVTGAAAGIGKATALRFAKEGAKVAAWDVADA
                     AALAEELKAAGAADVLVAKVNVADAASVNAAVAEVVAKWGRVDVLVNNAGIVRDAQLV
                     KYKDGTVQATMTDEQWDAVIGVNLKGVFHCTRAVTPHMIAAGGGVVLNASSVVGLYGN
                     FGQTNYVATKSGVIGMTKTWARELGKFRIRVNAVCPGFVATEILKAMPQKVLDGMVAH
                     TPIGRMGEPEDIANAYVWLASDQAAFVHGAVLSVDGGLVTGT"
     gene            complement(5638..8430)
                     /locus_tag="P5164_07770"
     CDS             complement(5638..8430)
                     /locus_tag="P5164_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRY43828.1"
                     /translation="MTRLTRNPFLRAAALFGAVLLPALTGAGPARAQTADATVEIDVV
                     DETAVALPGVTVELKRAETGFARTSVTSAKGAARFPAVPPAAGYVARVSLQGFEPVEQ
                     ALTLRIGQTAKLKVVLRAKALAESVTVVGEAPLVDVYKIDSSTNIVPEQIRELPVANR
                     EFEKLAFIAPGVQRERGEFRFVTGGPVIGSGGNASQSTILVDGVDYTDPALGLAKTRV
                     SQDAIAEFRVVNSRFDAEVGGSAGGALTVLTKTGTNELKGSLFAFYRAEGLRAKGALE
                     QESTVDFRRGQYGATLGGPIVKDKTHFFLSGELVDEVRPTLFRPQGAFKSQAADLEVP
                     TDQILAFGSVTHALTDAQSLLVKADYERYRQDNFRVGGVQDASYGQELQRDNYNFTAG
                     HTWVAGPRTTNELRAQFGHRKYFEPTNSDDVADWYTNGITLKTGGNILGDLLGEGDQL
                     ELRDTLYFHLTGGSGTHDVKVGAGWQWVKDRSIIDTYASGLFLWAGDTKAFPIAYGYG
                     VGSSDVEIRTNRIAAFVQDDWRPLSNLTVSAGLRYDLDTNGNNTGFTHPLVPEPRERD
                     SNNVQPRLGLSWDVKKDGRFVARAGWGLFVGRNLLVPSFTELQQNGVTGRKLYTRVNG
                     LLYGLPAFALDPKNPTTTGLPLAIDITLLDQELVAPEASQVSAGLTSRLWHTGLYLDV
                     EGVWVKGKKELIVHDVNWNGNANPTRPNKAYNQVNVYSNDGESEYKALTFALNGSLKG
                     GHLVTGSVTFASKYNRNDDFSPEFPTGYPSDPANLPNEWGRARSYERVRFVLSGVFRL
                     PWQLTLAPIFNYGSGQPWTSRYGYDYNGDGKASDRKPGFDRFGMDGPVYRSFDLRLTK
                     AFEIGSAGTLELVAEAFNLLNATNYDVASIDGAEFLRGPTLANPAAAAVANPNYGKPS
                     ATLPSREFQLGVRFAF"
     gene            complement(8649..>10146)
                     /locus_tag="P5164_07775"
     CDS             complement(8649..>10146)
                     /locus_tag="P5164_07775"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY43829.1"
                     /translation="KRERFLERKERILAEHKGLGRERLVDLLGEAELAFFALDPDKAM
                     ALLEEAWKLDRRNAELAFFLGETWFNDGDSGKAASALRHVLEQRPQHFDALVYSGVLE
                     NDLGRPEKAVDALKRALALRPDAFLPAFALGAIHAGAGQLAKAEALLKRAVAIEENPQ
                     ALSLLGTIAYERGRLADAIDALQRAVQLDPDDEEGLFQLGLCYLDRGWTQKAAERFRA
                     ALDLSPNRIELREAKKLLGPAGAGALPGLAGTTGERGGRTAAAAARDPRKAIGLYRRA
                     IKADPENPAVQIAYALVCSSVGRTAEAVATTRRVLSQKPDEEIAAAAWSTLLETLRAE
                     GKFREGTQVAGEMLQSVTTSSARSIAYFQRAAALADMGERLDEALEDAEKALRLSPRE
                     LRHFPLAAKGWVHYKRKEFRRAVECLSRAAELGETPTALAHLGLAYLAVGDAKAARSA
                     FERAKRRGGRLSERHGGLEARMLEQIRRNLQLAERVASRPGRRKPGAG"
BASE COUNT         1387 a         3564 c         3728 g         1467 t
ORIGIN      
        1 gccgaggacc ttcccgatga gcttgccgtc catcttcccc tcggcgtcgc gcatcttctc
       61 gaagccgacg gcgaggccga cgtccccctc gccgagcatc agcttctgcg cgacggcgag
      121 gatcgcctgc ccgcccgtgg cgcaggcgtt ctcgacgctc tcgatcggct tcgccccgag
      181 gcccggcgtc atcgcgagca ggcccgagag gaggccctgc tcgttgaggg tcaggttgca
      241 gacggcgccg atcgtggcga ggtcgacggt ggacgcctcg gccttcaccg agcggcacgt
      301 ctcctcggcg gccgtccgga tgatctcggg cacggtcgcg cccatgagct tcccgaactt
      361 cgactgctgg tacgcggcga tgtagatcgg cttcacgggg tccctccgtt ccgctcgggt
      421 tcgcgggccg ggccctcggc cgggagcggg tgccgggcga ggaaggcgag gaggcgggag
      481 agggtctcgc ggggattctc gacgacgagg ccgtgcccgc agccggcgac gacggcgagc
      541 tccccgcgcg ggagggccgc cgcgagcgac tcaccccacg ggcgcggcat cgcgcggtct
      601 ccgtccgcga ggaggacgag gacggggcag gccacgcgcg ggaggagcgg cctcacgtcg
      661 agcccctcga gcgccccgag gagagaggcg agaccggaga accaggagtc ggggaggagc
      721 gagacgagcg cgcggcggga ggcgatctcg tccgcgtggg cggcggccca ctccggagag
      781 aacgcgaagg ccgagacgag gtcgtagacc ttctcgcgcg acccccccgc gagcgcctcg
      841 cggcacgcga cggccatccg gtccgcctcg acccacatct cgggcgagac cctgtccgcg
      901 acggtcatcg cgacgagaga ggccacgcgt tccggcgcga gcgccgcgag gtggaggccg
      961 gcgaaggcac cgtagctcgt cccggcgacg tgacactgcg cgaccccgag gtgatcgagg
     1021 agccggagga ggtcctccgc gtgcccgggg aacccctcgg gccgatcgcc cggcgagcgg
     1081 agcatcccgc gaaggtcgca ccggatcacc cggtaccgtt ccccgagcgg cgggacgtac
     1141 gcgtcccacg ccgtgtagct catcatcccg ccgttcagga gaaggagcgg ctcgccctcg
     1201 ccgtcgacct ggcgcgcgag gagggcggga gcgttcacga ggcgcccctg ccggcgaggt
     1261 aggcgtcccg aagctccccc ttgacgacct tcccgtaggc cgtccgcggg aggtccccga
     1321 cgaagacgac ctccttcggg agcttgtacc gggcgagacg cggctcggcg aacgcgagga
     1381 gctccgctcc cgtggcgctc gcgccggggc ggaggacgac gaaggcgacg ccgacctcgc
     1441 cccaggtccg gtcggggacg ccgacgaccg ccgcgtccat gacagccggg tggaggagga
     1501 gctccccttc gatctcggcc gggtagacgt tgacgccgcc ggagatgagc atgtccttgc
     1561 ggcgtcccgc gatcgtgaag aagccgtcgg cgtccttccg cgcgaggtcg cccgtgcgga
     1621 agaagccgtc gtcgtcgagg gcggccgccg tcgcctcggg gttcctccag tatcccttcg
     1681 tgacgtgcgg gccgcggagg cagagctccc cgacctcgcc ggcgggcacc tcggagcccg
     1741 actcgtcgac gagcttcgct tcggtcatca tgagaggcct gccgatcgac cccttcttcg
     1801 tgatcgactc ctcgaccgac atcgcgaagc agttcacgcc cacttcggtc aggccgtacc
     1861 cctgcttgaa gacgatccct cgagcctggt aggcctcgat gagatagagc gggagcggcg
     1921 cgccgccgct gatgagccag cggatcgagg agaggtcggc ccgctcgaac gacgggtgct
     1981 cgaggagcag cttccagatc gtcggaacgc cgaggacgac ggtgcagcgc tccctcgcca
     2041 ccgtcgccca gatctccgcc gcgtcgaacc cggcgtggag gacgaccgtc ccccccgccg
     2101 ccaggatcgg cgcgaagaag gccccgagcc cgcccgcgtg gtagaggggc gtgatgaccg
     2161 ggctgacgtc gtgctcggtc aggccccagc agagcgcggt attccaggcg ttccaggagg
     2221 tcatccggtg cgggaccatg acccccttcg gccggccggt cgtcccgctc gtgtagagga
     2281 ggaggtggag gtcctcgccg tcgcagcgcg tccgctcgaa ggagacgtcg gggagcgcgg
     2341 cgagccccgt cgcgagcggg aggtcccctt ccgtcgcggg cgcgtcgagt gcgatccgcc
     2401 gcggcatctc cgcctcggcg cggagggcgg cgaccgtctc tgcgaagtcg cccccgtaga
     2461 cgagcgccga gacgtccgcg ttccggacga tgaacgcgag ctcgtgaggc gtcagccgcg
     2521 ttccgagcgg gacgaggacg agccccgcct tcgctgcggc aaagaagagt tcgacgtact
     2581 cgacgcggtt gtgggcgagg agggcgacgc ggtccccctt cacgaggccc agcccgtcgc
     2641 ggaggaacgc cgcgacgcgc tcggcgcgcg cgttcagggc ccggtaggtg aaccgctcgc
     2701 cggtcgggac gaagacgagg gcgcacctat ccggagagag gcgcgcccgc tcgccgagga
     2761 ggtcggcatg gatcacgggg cctccggcgc gacggccggc cccggaagcg gtccgtcgag
     2821 cagcttcccg agggcggccg cgaaggcggc cgggcactcc tgctgcggga cgtgcccgca
     2881 gcgcggcagc tcggtgacgc gcgcccgcgg cagctcggcg cgcatcctct cggcgtagga
     2941 gagcggcagg agctggtcgg aggcgcccca gaggaggtcg atcgggacgt cgagacctcc
     3001 gagcttcccg tcgaggacga agggctccat ctcgccgctc gtcgccgcga ggcgcgcgag
     3061 ggggcccgtc gccgcctgcc tcacgacgtc gtcgaggacg aagtcgggga cccggatcga
     3121 gcccgggtcg cggagcttct ccaccgcggc ccgcgcctcg gcccggtcct tcgggaggag
     3181 cgtgatcccc tcggcggcgt gcttcagcgg gccgccgttg acgaggacga ggcgctcgac
     3241 acgctccggg tgctccttcg cccagaggca ggcgagccag gccccgagcg agttgccgac
     3301 gatcgtcacc tttccggcgg gggccgccgc atcgacgacg gccgagagtc cggcgaggag
     3361 cgtcgggacg gagatcgggc cggccgcggg cgcgctcgcg ccgtggccgg cgaggtcggg
     3421 gacgacgagc gtccgcccgg ggagaagctt cggggcgacg agcgaccagg ccgaggcctg
     3481 gtcgcccgcg ccgtggagga ggacgaggac gggcccgctc cccccgcggt agtaggactg
     3541 ctcgcccgcg ggcgacgcga ctcgggccct ctcgaggccc gacttcgcga gcgcgcggcg
     3601 gcccgccgcg gcgtacgccg cgaggggcct catccgcgcc cagaggaacg cggcgaccgc
     3661 cgcgaggacg aggagggcgg ccgcggcgag gaggagcttc acgggcttgt tcatcgcgat
     3721 ctccgcgccg cggcccggcc ctcaggtcat ccggatcgcg acgccggcct ggttgtaccc
     3781 cacgccggag ccgatcatca cgacgaggtc gcccttcttc accttcccct gctcgagggc
     3841 gtcgtcgagg gccatcggga tgcaggccga gccggtgtag ccccacttct ccatgatcgt
     3901 gtgggccttc tcgaccggga ggccgaggtc gttcatgacg agctcgatgg agggcttcct
     3961 cacctgcgtg aagatgacct ggtcgacgtc ggagagggcg aaaccgccct cgtgggcgag
     4021 ccgccggacg agcctcggcc acccctcgtg gttgatctcc gggggatacc tctcgaccat
     4081 ccggacggtc gtcttcccgg ccgcgacgtt ctcgaccgtc gccggctcgg cggtgccgcc
     4141 cgcgaagatg ccccagcact tcgcgtactc gccgcccgcc tgcatcgcgg cgttcaggaa
     4201 gccgggctcg tcgcccccct ggaggaccgc ggcccccgcg ccgtcgccgt agaagaagac
     4261 catcgggtcg ttcgggtcgg cgagcttgtg catcaggtag acgccgacga cgaggacgtt
     4321 ccggagggcc gggttcgtgg cgatgaggcc ggccgccgcg gcgacccccg tcgggaacga
     4381 cgcgcaggcg cagccgacgt cgaacgtccc ggcgttcttc gcgccgagct tcccctggag
     4441 gacgacggag gtcgccggcg tgacgtagtc gggcgagtcg gtgccgagga tgatcaggtc
     4501 gaggtcgagc ggggagaggc cggcccgctc gagggcctgc ctcgccgcgg ggagcgcgac
     4561 gtcggacgtc gcccagtcct cgggggcgta gaagcgggtc cggatggcgc tcgaggcctc
     4621 catcttgtcg acgaagtccg ggagctgcgg ccagcgcggc ttcagggccg agttcggcac
     4681 ctcgatcggc gggacgaacc gcccggtcga gacgatgcgc gcgtagcggg ccacggcgct
     4741 aggtccccgt gacgaggccg ccgtcgaccg agaggacggc gccgtggacg aacgccgcct
     4801 ggtccgacgc gagccagacg tacgcgttcg cgatgtcctc gggctcgccc atccggccga
     4861 tcggcgtgtg ggcgaccatc ccgtcgagga ccttctgcgg catcgccttc aggatctccg
     4921 tcgccacgaa gccggggcag acggcgttga cgcggatccg gaacttcccg agctcgcgcg
     4981 cccacgtctt cgtcatcccg atgacgccgc tcttcgtcgc gacgtagttc gtctggccga
     5041 agttgccgta gaggccgacg acggaagagg cgttcaggac gacgccgccc cccgccgcga
     5101 tcatgtgcgg ggtcacggcg cgcgtgcagt ggaagacccc cttcaggttc accccgatca
     5161 cggcgtccca ctgctcgtcc gtcatcgtcg cctgcaccgt gccgtccttg tacttgacga
     5221 gctgggcgtc cctcacgatc ccggcgttgt tgacgaggac gtcgacccgg ccccacttcg
     5281 cgacgacctc cgcgacggcc gcgttcacgg acgccgcgtc ggcgacgttg accttcgcga
     5341 cgaggacgtc ggccgcgccc gcggccttca gctcctctgc gagagccgcc gcgtccgcga
     5401 cgtcccaggc ggcgaccttc gccccttcct tcgcgaagcg gagggcggtg gccttcccga
     5461 tcccggcggc cgcgccggtc acgacgaccg ctcggcccgc gagtcccgtt tcgatgctca
     5521 tggcttcctc tttcctgccg cggcgcgcag gccgcgggcg aggccccgcg tcgcgcgggg
     5581 cgtggatccg gcggacggca gggccgcccg ccggatgatt ccgaccgacc gcgcctccta
     5641 gaaggcgaac ctcacgccga gctggaactc ccgcgaaggc agcgtcgcgc tcggcttgcc
     5701 gtagttgggg ttcgcgacgg ccgccgccgc cgggttcgcg agcgtcgggc ctctgaggaa
     5761 ctcggcgccg tcgatcgagg cgacgtcgta gttcgtcgcg ttgaggaggt tgaacgcctc
     5821 ggccacgagc tcgagggtgc cggccgagcc gatctcgaac gccttcgtga gcctcaggtc
     5881 gaagctccgg tagacgggcc cgtccatgcc gaaccggtcg aagccgggct tccggtcgct
     5941 cgccttgccg tcgccgttgt agtcgtagcc gtatcggctc gtccagggct gaccggagcc
     6001 gtagttgaag atcggcgcga gggtgagctg ccacgggagg cggaagacgc cggagaggac
     6061 gaagcgcacc cgctcgtagg agcgggcccg gccccactcg ttcggaaggt tcgccgggtc
     6121 gctcgggtag cccgtcggga actcggggct gaagtcgtcg ttccggttgt acttgctcgc
     6181 gaacgtgacc gagcccgtga cgaggtggcc gcccttgagg ctgccgttca gcgcgaaggt
     6241 gagcgccttg tattcggatt cgccgtcgtt cgagtagacg ttgacctggt tgtaggcctt
     6301 gttcggacgg gtcggattcg cgttcccgtt ccagttgacg tcgtggacga tcagctcctt
     6361 cttccccttg acccagactc cctcgacgtc gaggtagagg ccggtgtgcc agaggcggga
     6421 ggtgaggccg gcgctgacct ggctcgcctc gggcgcgacg agctcctggt cgaggagcgt
     6481 gatgtcgatc gcgagcggga ggccggtcgt cgtcgggttc ttcgggtcga gggcgaaggc
     6541 cggcaggccg tacaggaggc cgttcacccg cgtgtagagc ttccgccccg tgacgccgtt
     6601 ctgctggagc tcggtgaacg acggaaccag gaggttgcgg cccacgaaga ggccccagcc
     6661 cgctcgcgcg acgaagcggc cgtccttctt cacgtcccac gagagcccga ggcggggctg
     6721 gacgttgttc gagtcccgct cgcgcggctc cgggacgagg ggatgggtga agcccgtgtt
     6781 gttcccgttc gtgtcgaggt cgtagcggag gccggcgctc acggtcaggt tcgagagcgg
     6841 ccgccagtcg tcctggacga aggcggcgat ccggttcgtc cggatctcga cgtcgctcga
     6901 gccgacgccg tagccgtagg cgatcgggaa agccttcgtg tcacccgccc agaggaagag
     6961 cccgctcgcg taggtgtcga tgatcgagcg atccttcacc cactgccagc cggcgccgac
     7021 cttcacgtcg tgcgtgccgc tcccgccggt caggtggaag tacagggtgt cgcgcagctc
     7081 gagctggtcg ccctcgccga ggaggtcccc gaggatgttc ccgcccgtct tcagcgtgat
     7141 cccgttcgtg taccagtcgg cgacgtcgtc ggagttcgtc ggctcgaagt acttgcggtg
     7201 gccgaactgc gcccgcagct cgttcgtcgt cctcgggccg gcgacccagg tgtgccccgc
     7261 cgtgaagttg tagttgtcgc gctggagctc ctgtccgtag gaagcgtcct gcaccccgcc
     7321 gacccggaag ttgtcctggc gataccgctc gtagtccgcc ttcacgagga gcgactgcgc
     7381 gtccgtgagg gcgtgggtca cggagccgaa cgcgaggatc tggtcggtcg gcacctcgag
     7441 gtcggcggcc tggctcttga aagccccctg cggccggaag agggtggggc gcacctcgtc
     7501 gacgagctcg cccgagagga agaagtgggt cttgtccttg acgatcgggc cgccgagcgt
     7561 cgcgccgtac tgcccgcggc ggaagtcgac ggtcgactcc tgctcgagcg cccccttcgc
     7621 ccggagcccc tcggcccggt agaacgcgaa gagcgagccc ttgagctcgt tcgtccccgt
     7681 cttcgtcagg accgtcagcg cgcctcccgc ggagccgccg acctcggcgt cgaagcgcga
     7741 gttgacgacg cggaactcgg cgatggcgtc ctgcgagacg cgggtcttcg cgaggccgag
     7801 cgccgggtcg gtgtagtcga cgccgtcgac gaggatcgtg gactgggagg cgttcccacc
     7861 cgagccgatg accggcccgc ccgtgacgaa gcggaactcc ccacgctccc gctggacgcc
     7921 cggcgcgatg aacgcgagct tctcgaactc gcggttcgcg acggggagct cccggatctg
     7981 ctcggggacg atgttcgtcg aggagtcgat cttgtagacg tcgacgaggg gggcctcgcc
     8041 gacgaccgtc accgactccg cgagcgcctt cgcccggagg acgaccttca gcttcgccgt
     8101 ctgaccgatc cggagcgtga gcgcctgctc gaccggctcg aaaccctgga gcgagacgcg
     8161 ggcgacgtag cccgccgccg gcgggactgc cgggaaccgg gccgccccct tcgcggaggt
     8221 gactgacgtc cgggcgaaac cggtctcggc gcgcttcagc tccacggtga cgccggggag
     8281 cgcgacggcc gtctcgtcga cgacgtcgat ctcgacggtc gcgtccgccg tctgcgcccg
     8341 ggcgggaccg gccccggtca gggccggcag gagaacggcc ccgaacagag ccgcggcgcg
     8401 aaggaagggg tttcgggtca gcctcgtcat ctcgatcctc ccgggcgccg tcgcgccctc
     8461 gtcaagcgtg tggcctgccg cggctcgcgg ggagcgggcg aagcgataga gccggtcccg
     8521 ggccacgccc gcgcttcgtc cgcaccgcct tcaacaaaat accgaacact cggtatgttt
     8581 ttacgaaccg attctgggaa gcctcggagg agcctgtcaa gagaaatcga ccgcagggcc
     8641 ggcgcgattc agcccgcccc gggctttcgg cgtccgggtc ggctggcgac ccgctccgcc
     8701 agctgcaggt ttcgccggat ctgctccagc atgcgcgcct cgaggccgcc gtggcgctcc
     8761 gaaaggcgtc cgccccgccg cttggcgcgc tcgaaggcgc tgcgggcggc cttcgcgtcg
     8821 ccgacggcga ggtacgccag gccgaggtgc gcgagcgccg tcggggtctc cccgagctcc
     8881 gccgcccgcg agagacactc cacggcccgg cggaactcct tcctcttgta atggacccac
     8941 cccttcgccg cgagcgggaa atgccggagc tcgcggggag agaggcggag ggccttttcg
     9001 gcgtcctcga gcgcctcgtc gagcctctcc cccatgtcgg ccagggcggc ggctcgctgg
     9061 aagtaggcga tcgagcgggc cgagctcgtg gtgacggact ggagcatctc gcccgcgacc
     9121 tgggtccctt cccggaactt cccttcggcc cggagcgtct cgaggagcgt cgaccaggcc
     9181 gcggcggcga tctcctcgtc gggcttctgc gagaggacgc ggcgcgtcgt cgccacggcc
     9241 tccgccgtcc gtccgacgga ggagcagacg agggcgtagg cgatctggac cgccgggttt
     9301 tccgggtccg ccttgatcgc ccgccggtag aggccgatcg cctttcgcgg gtctcgcgcc
     9361 gccgcggcgg cggtgcgccc cccccgctcg cccgtcgtcc cggcgagacc gggcagggct
     9421 cccgcgccgg ccgggccgag gagcttcttc gcctcccgca gctcgatccg gttcgggctg
     9481 aggtcgagcg ccgcacggaa tcgctccgcg gccttctggg tccagccccg gtcgaggtag
     9541 cagaggccga gctggaagag cccctcctcg tcgtcggggt cgagctggac ggcccgctgg
     9601 agcgcgtcga tggcgtcggc gagacgtccc cgttcgtagg cgatcgtacc gaggagcgag
     9661 agcgcctggg ggttctcctc gatcgcgacc gccctcttca gcagcgcctc ggccttcgcg
     9721 agctgcccgg ccccggcgtg gatcgcgccg agcgcgaacg cgggcaggaa cgcgtccggc
     9781 cggagggcga gcgcccgctt cagggcgtcc acggccttct ccgggcgccc caggtcgttc
     9841 tcgaggaccc cggagtagac gagagcgtcg aagtgctggg ggcgctgctc gaggacgtgc
     9901 cggagggccg aggccgcctt tccggagtcg ccgtcgttga accaggtctc gcccaggaag
     9961 aacgcgagct cggcgttgcg gcggtcgagc ttccaggcct cctcgaggag agccatcgcc
    10021 ttgtcgggat cgagcgcgaa gaaggcgagc tcggcctcgc cgaggaggtc gacgaggcgc
    10081 tcccgtccga gccccttgtg ctcggcgagg atccgctcct tccgctcgag gaagcgctcg
    10141 cgcttc
//
LOCUS       DAOTEZ010000114        10114 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4694_length_10114_cov_3.871558, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000114 DAOTEZ010000000
VERSION     DAOTEZ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10114
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4694_length_10114_cov_3.871558"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="P5164_07780"
     CDS             <1..448
                     /locus_tag="P5164_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43830.1"
                     /translation="MVGILQLFSLALLTDTGSASRSDMTLRAQQVAENLRYLHFLRTQ
                     DPAAVPANTGLPTSPTNGATVTLPRNSTTDTNKAWWLYWGPAGTNVMYQENPPFSISY
                     TYSASPTPGFWFVTVSVTAADAPTGMSYIGTSARTKRVDYVCQVPQ"
     gene            480..1712
                     /locus_tag="P5164_07785"
     CDS             480..1712
                     /locus_tag="P5164_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43831.1"
                     /translation="MVSLLILTMVMIVALTLLFTMKSFAERQQVKTAPRQTSRRAIDY
                     LSTHIAGATDLNDNGPTPSPNALIAWYAQGNSNTPVQATYNNVTDGRLAEPGTDILTL
                     AAPIDPFMVPISRWVGWQKAANLYFHYTEGCPSDETMRRNFLQLTGASSEGNDAQSII
                     LTLVDRDGRWAYYEITGYQDFSCTPKTSDEGTPYNVHVVSNPGQSQRINPPGGHPDLN
                     DPALAGGMRIYSFRVRRDANGILNLEQKQGLFNPATDNPGTAFTPIMPDVEDLQIAYL
                     YSRPPDPTNFPGRTVFNTFDAATGADVRIPATGNVPPQAAVGSATPWDITMVSGIRIS
                     ITSRSIPLRFEARNASARRNSSTTTLLSGARPRSEDRAPAAPSDADPTGPSGVLIGDF
                     DHHRMTSTVLIRNRMLGN"
     gene            1716..2414
                     /locus_tag="P5164_07790"
     CDS             1716..2414
                     /locus_tag="P5164_07790"
                     /inference="COORDINATES: protein motif:HMM:NF025699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HRY43832.1"
                     /translation="MSRSRPPRSAPSRRPSARGSALITVILVALVLTVVGLGIAYFAS
                     TEDKTSGNTEMARVGFYAAEAGLRDGEARVSDFAQLNNSDATELIDLTYSSGAAIDAR
                     PWPAGDVYTPPGGGRPAYVLEIAGTKYRDIVIPQDLGNPSTRAMYSIFVRNNEEDTAG
                     GNNADTDKKINIVVVGQMVLVDPSTKALVLRDGVPTIGITKVLEEQLYTEVEGSATAT
                     QKGTNVGGTSSGAK"
     gene            2435..>4726
                     /locus_tag="P5164_07795"
     CDS             2435..>4726
                     /locus_tag="P5164_07795"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43833.1"
                     /translation="MRTSRIHLLPSALVLAAALAAPGLRAQDEGYDPMRQITAQLQKA
                     NLQIVLDVTGSMAWFPEGKVTPETGVNSFGWLRWNRDQYGDSWGPSGSSCSSGKCYTW
                     KYKLAFNGPSRMAIVKNALGASVPLVTTYTPPEELPARWTLESTASSTASRREWVFRY
                     DATSRVKAPGIPFTPYETITIAGARNSSGGDYTVDYFPIVRNGACTVGTCTYSAPQDL
                     IGKNAARVNWGLTTYSTDGITHRVDIDVTDANEYLAKLKDYFLPSGATGRATGVGGLG
                     ASGGTNTNTALSRVKTKITTIAGLDPRIRENCDRPYGVILVTDGLSNNSNPDDGNWIS
                     PCGTGPLECDGGSSGYDCPNNWDKFAAKWANELYTNTSAGSVQVPVRTWTIGVSEQVG
                     PCELDYIAFMGRTDASSPNGDAGWGGFDPVKNPFLPDPASPEPANPRYNTTSNFDGPS
                     GQLRWNQDSSATFATALAGYGDPVKKAHGHNAFFATSAEKLGEALMAIVNATATGDYA
                     TNAPVSGMSAGGSLASTVFLPSTEFPSWKGHIYAFDTSKLAKIDHDGNPATPMIDNPD
                     YLKWDAGVVLNPSTAHATRKIFTWDPANGNALVELKSDNLDRLNDADMGGLSFDAAVL
                     DFVRGAPRDFDGDGVTDSRDWLLGPMINSAPALVGRPAAWLQGNTVSHSNFENAYASR
                     EPLLWVGSNNGMMHAFRIEDGVEQIALVPPALLKMQVQLFENYLADERLKKNPFGQPP
                     DPAQHVYGVANSFRFGDVWDPDNS"
     assembly_gap    4727..4736
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4737..5953
                     /locus_tag="P5164_07800"
     CDS             <4737..5953
                     /locus_tag="P5164_07800"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43834.1"
                     /translation="YRTVGVITVGAAGDDVVAVDVTSVPSPTAATYPTDPVKVLWAKD
                     STTVTGLKQTWSIPAMAPATSTTWRMVLGGGFNPSNTRAAQISGTGFTAPKAFTLDPT
                     DGSILGTITLNSSTYTPWVGNQAFADSVIFDPSAKVYQDDNIAKLGLQADLNGQIWFL
                     YNQPSNKDDFATARVGIDVSAKAGQSQPIYYNPAASGYGVGGAGCVAYAFGSGALYEK
                     SAFVTGPDVGTAGNFIPQLYVATGAKGSFTSALSAGNIVGKPIATSWCIANCEDPDPT
                     THVYRTFGTKTQLTAPPFMLVPRSGRGSVTALFLLYDPEYGCHGNSYIAIVDFAGNDT
                     CSLGSSDVTFKAVDAGEGAASGFTIAGNKVLVSKSGIGQGERAHLTSPPDIAASIGGP
                     APPRVKWWKELK"
     gene            7066..7257
                     /locus_tag="P5164_07805"
                     /pseudo
     CDS             7066..7257
                     /locus_tag="P5164_07805"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
     gene            7495..8577
                     /locus_tag="P5164_07810"
     CDS             7495..8577
                     /locus_tag="P5164_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HRY43835.1"
                     /translation="MATPTLQDFLADTVSKGASDFHLATGFVPHQRIHGRLVPMGTPL
                     MTPEVTEGLIRALLTPEEWDELLARYNLDVCIKVPGLGRFRANFFSQRAGYDACFRVI
                     PSSVPTLSDVGLPESAWELARFPHGLVLVTGPAGCGKSTTLAALLNLVNETRRGHIIT
                     IEDPVEFIHPNKECLVNQRQVPHHTESFARALRAALREDPDVILVGEMRDIETIALAI
                     SAAETGHLVFGTLSTTTAPATVDRIINAFPAGQQGQIRSMVSESLKAVISQTLLPRRD
                     GIGRIAAFEILRGTSAVGAMIRESKTYQLPGILQTGQLSGMNTMDQALLKLAEERKVD
                     AEAAMDRAQRKEPFEKLVAEERLGPS"
     gene            8574..9677
                     /locus_tag="P5164_07815"
     CDS             8574..9677
                     /locus_tag="P5164_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HRY43836.1"
                     /translation="MSAAPPQTRARIDSLLRVVLEKKASDIHLTCGSPPMLRIDGELT
                     RMKWRSIREEDFENLLRPIAPGWVWEEFTSTGDGDFAYAMGDKARFRVNLFRQEHGSG
                     AVLRMIPPRVLTIEDLGLPEQVASVGRTQRGLVLITGPTGSGKSTTLAALIDRINRTL
                     PYHIVTIEDPVEFVHVPAKSIITHREVGENTPSFTTALKAALREDPDAILVGELRDIE
                     TMRMALHAAETGLLVFGTLHTNSAAKAIDRLIDGFPSDEQEQVRIVLSEVLKAVVAQI
                     LMRKKGGGRVPAFEVVRGSAALANAIREGKTGTIASLIQTGRSHGMISLDQSLADLVV
                     ADLVDADEASEYAVDRDYFRSLVDKKRNPEAPA"
     gene            complement(9688..>10114)
                     /locus_tag="P5164_07820"
     CDS             complement(9688..>10114)
                     /locus_tag="P5164_07820"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY43837.1"
                     /translation="LVEEILAEGRATCPGAAFSAEGDFPRVEADAALLRRALVNLVRN
                     AAEAAGDGGRVAVRGEPSDGGGVALVVEDSGPGVAPEAAEKLFVPFASTKEGGTGLGL
                     SLVAKIAALHGGSVAASRSAALGGAAFRLALPPPAPTRP"
BASE COUNT         1678 a         3727 c         3322 g         1377 t
ORIGIN      
        1 gatggttggc attctccagc tcttctccct cgctctcctg acagacaccg ggtcggcctc
       61 acggtctgac atgacgctcc gcgcgcagca ggttgccgag aacctccgtt atctccactt
      121 cctccggacg caggacccgg ccgccgtccc ggcgaacacg ggactgccga catcgccgac
      181 gaatggcgcc accgtgacgc tgccgaggaa cagcacgacc gacacgaaca aggcgtggtg
      241 gctctactgg ggtccggccg ggacgaacgt catgtaccag gagaaccccc cgttctccat
      301 cagctacacc tactccgcgt cgccgacgcc gggcttctgg ttcgtcacag tgtcggtcac
      361 cgccgccgac gcccccaccg gaatgagcta catcgggacg agcgcccgaa cgaagagggt
      421 cgactatgtt tgccaggttc cgcagtaggc gcatccgcgg catctcgctg tcggaagcga
      481 tggtgtcgct cctcatcctg acgatggtga tgatcgtcgc gctgacgctt ctcttcacga
      541 tgaagtcgtt cgcggagcgc cagcaggtga agaccgctcc ccgccagacc tcccgccgcg
      601 cgatcgacta cctctccacg cacatcgccg gcgcaaccga cctgaacgac aacggcccga
      661 cgccgagccc gaacgccctc atcgcctggt acgcgcaggg gaactcgaac actccggtcc
      721 aggctaccta caacaacgtc accgacggac ggctggccga gccggggacc gacatcctca
      781 cgctcgccgc cccgatcgac ccgttcatgg tcccgatctc gagatgggtc ggctggcaaa
      841 aggccgcgaa cctctacttc cactacaccg agggctgccc gtcggacgag acgatgagga
      901 ggaacttcct ccagctcacc ggcgcctcga gcgaggggaa cgacgcccag agcatcatcc
      961 tgacgctcgt cgaccgggac ggccgatggg cctactacga gatcaccggg taccaggact
     1021 tcagctgcac gccgaagacc tcggatgaag ggacccccta caacgtccac gtcgtcagca
     1081 acccggggca gtcgcagcgg atcaacccgc cgggcggcca cccggacctc aacgatcccg
     1141 ctctggccgg cgggatgcgg atttactcgt tccgggtccg ccgcgacgcg aacggcattc
     1201 tgaacctgga gcagaagcag gggctcttca acccggcgac ggacaacccg gggaccgcgt
     1261 tcacgccgat catgccggac gtcgaggacc tccagatcgc gtacctttac tcccgccccc
     1321 ccgacccgac gaacttcccc ggacggaccg tcttcaacac gttcgacgcc gcgacgggcg
     1381 ccgacgtgag gatcccggcc acggggaacg tccccccgca ggcggcggtc gggagcgcga
     1441 ccccgtggga catcacgatg gtctccggga tccggatctc catcacctcc cgatccatcc
     1501 cgctccgctt cgaggcgcgg aacgcctcgg cgcgccggaa ttcgagcacc acgacgctcc
     1561 tctcgggcgc ccgtccccgt tccgaggacc gggctcctgc tgccccgagc gacgccgacc
     1621 cgaccgggcc ttcgggcgtt ctgatcggcg acttcgacca tcaccggatg acctccaccg
     1681 ttctgatccg gaaccggatg ctggggaact agacgatgag ccgcagtcga ccgccccgct
     1741 ccgctcccag ccggcgcccc tctgcccggg ggagcgccct gatcaccgtc atcctcgtcg
     1801 ccctcgtcct gacggtcgtg ggcctcggga tcgcctactt cgcctcgacg gaggacaaga
     1861 cctcgggcaa caccgagatg gcccgggtcg gcttctacgc cgccgaggcc ggactccgcg
     1921 acggcgaggc gcgcgtctcc gacttcgcgc agctcaacaa cagcgacgcg accgagctca
     1981 tcgacctgac ctattccagc ggcgccgcga tcgacgcgcg gccatggccc gccggcgacg
     2041 tctacacgcc ccccggcggg ggccgccccg cctacgtcct cgagatcgcc ggaacgaagt
     2101 accgggacat cgtcatcccg caagacctcg ggaacccctc gacgcgggcg atgtactcga
     2161 tcttcgtccg gaacaacgag gaagacaccg ccggcggcaa caacgccgac accgacaaga
     2221 agatcaacat cgtcgtcgtc ggccagatgg tcctcgtcga cccgtcgacg aaggcattgg
     2281 tcctccgcga cggcgtcccg acgatcggga tcacgaaggt cctcgaagag cagctctaca
     2341 ccgaggtcga gggctccgcc accgccaccc agaaggggac gaacgtcggc ggcaccagct
     2401 ccggcgcgaa gtgaacggta ccaaggagat cgccatgcga acctcgcgaa tccaccttct
     2461 cccttcggca ctcgtcctgg ccgccgctct cgccgccccg ggcctccgcg cgcaggacga
     2521 gggctacgat ccgatgcgcc agatcacggc ccagcttcag aaggcgaacc tccagatcgt
     2581 cctcgacgtg acaggctcga tggcgtggtt cccggaggga aaggtcacgc cggaaacggg
     2641 cgtcaacagc ttcggctggc tgagatggaa ccgggaccag tacggcgaca gctgggggcc
     2701 gagcggctcc agctgcagca gcggcaagtg ctacacatgg aagtacaagc tcgccttcaa
     2761 cggcccctcc cggatggcga tcgtcaagaa cgcgctcgga gcctcggtgc ctctcgtcac
     2821 gacctacacg ccgccggaag aactgccggc ccgatggacg ctcgagagca cggcctcctc
     2881 gacggcgtcc cgccgtgaat gggtcttccg ctacgacgcc acgtcgaggg tgaaggcccc
     2941 cggcatcccc ttcacgccct acgagacgat cacgatcgcc ggcgcgagga actccagcgg
     3001 cggcgactac acggtcgact acttccctat cgtccggaac ggcgcctgca ccgtcgggac
     3061 ctgcacctac tcggcgccgc aggacctgat cgggaagaac gccgccaggg tcaactgggg
     3121 gctgacgacc tactccaccg acgggatcac ccaccgcgtc gacatcgacg tcacggacgc
     3181 caacgagtac ctcgccaagc tcaaggacta cttccttccg agcggggcga cggggagggc
     3241 caccggcgtc ggcggcctcg gcgcgagcgg cgggacgaac acgaacaccg cgctgtcgcg
     3301 ggtcaagacg aagatcacca ccatcgccgg actcgatccc cggatccgcg agaactgcga
     3361 ccgcccgtac ggcgtcatcc tcgtgaccga cggcctctcg aacaacagca accccgatga
     3421 cgggaactgg atcagcccct gcggcacggg cccgctcgag tgcgacggag gctcctccgg
     3481 ctacgactgc ccgaacaact gggacaagtt cgcggccaag tgggcgaacg agctctacac
     3541 gaacacctcc gccgggagcg tccaggtccc cgtccggacc tggacgatcg gcgtgagcga
     3601 gcaggtcggt ccctgcgagc tcgactacat cgccttcatg gggcggacgg acgcctcgag
     3661 cccgaacggc gacgccggat ggggcggctt cgacccggtg aagaacccgt tcctcccgga
     3721 ccccgcgagc ccggagcccg cgaacccccg ctacaacacg acgtcgaact tcgacgggcc
     3781 gagcgggcag ctgcgctgga accaggacag cagcgccacc ttcgccacgg ccctcgccgg
     3841 gtacggcgac cccgtgaaga aggcccacgg ccacaacgcc ttcttcgcca cctccgccga
     3901 gaagctcggc gaggcgctga tggcgatcgt gaacgccacg gcgaccggcg actacgcgac
     3961 gaacgccccg gtctccggga tgtccgcggg cggctccctc gccagcaccg tctttcttcc
     4021 gtccacggag ttcccttcgt ggaaggggca catctacgcc ttcgacacct cgaagctggc
     4081 caaaatcgac cacgacggca accctgcgac gccgatgatc gacaacccgg actacctcaa
     4141 gtgggacgcg ggcgtggtac tgaacccctc cacagcccac gccacgcgga aaatcttcac
     4201 ctgggacccc gcgaacggga acgccctcgt cgagctcaag tccgacaacc tcgaccggct
     4261 gaacgacgcg gacatgggcg gcctcagctt cgacgccgcg gtcctcgatt tcgtccgcgg
     4321 cgccccgcgt gacttcgacg gagacggggt cacggacagc agggactggc tcctcgggcc
     4381 gatgatcaac tcggctccgg ccctcgtcgg ccgtcccgcg gcctggctcc aggggaatac
     4441 ggtcagccac tcgaacttcg agaacgccta cgcctcccgg gagcccctcc tctgggtcgg
     4501 ctcgaacaac gggatgatgc acgcgttccg gatcgaggac ggcgtcgagc agatcgccct
     4561 cgtccccccc gcgctcctga agatgcaggt gcagctcttc gagaactacc tcgccgatga
     4621 gaggctgaag aagaacccat tcggacagcc tcccgacccg gcccagcacg tctacggcgt
     4681 ggcgaactcg ttccgcttcg gggacgtctg ggaccccgat aacagcnnnn nnnnnnccta
     4741 ccggaccgtc ggcgtcatca cggtgggagc cgcgggcgac gacgtcgtcg ccgtcgacgt
     4801 cacgagcgtc ccgagcccga cggccgccac ttaccccacc gacccggtga aggtcctctg
     4861 ggcgaaggac tccacgacgg tcacgggcct gaagcagacg tggtcgattc cggcgatggc
     4921 cccggcgacg tcgacgacct ggcgcatggt ccttggcggc ggcttcaacc cctcgaacac
     4981 ccgggccgcg cagatctccg ggaccggatt cacggccccg aaggcgttca cgctcgaccc
     5041 gaccgacggc tccatcctcg gcacgatcac cctgaactcg tcgacctaca caccctgggt
     5101 cggcaaccag gcgttcgccg actccgtgat cttcgacccc tcggcgaagg tctaccagga
     5161 cgacaacatc gcgaagctgg gcctccaggc cgacttgaac gggcagatct ggttcctcta
     5221 caaccagccg tcgaacaagg acgatttcgc gacggcgcgc gtcgggatag acgtctcggc
     5281 gaaggcgggt cagtcccagc cgatctacta caacccggcg gcaagcggct acggggtcgg
     5341 cggggccggc tgcgtcgcat acgccttcgg aagcggcgcc ctctacgaga agagcgcgtt
     5401 cgtcaccggt ccagacgtcg gcaccgcggg caacttcatc ccccagctct acgtcgcgac
     5461 gggcgcgaag gggagcttca cctccgccct gtcggccgga aacatcgtcg gcaagccgat
     5521 cgcgacctcc tggtgcatcg cgaactgcga agacccggac ccgacgaccc acgtctatcg
     5581 gacgttcgga acgaagaccc agctgacggc gccgccgttc atgctcgtcc cccgaagcgg
     5641 ccgcggcagc gtgaccgcgc tcttccttct ctacgacccc gagtacggct gccacggcaa
     5701 ctcgtacatc gcgatcgtcg acttcgccgg gaacgacacc tgctcgctcg gctcgagcga
     5761 cgtgacgttc aaggcggtcg acgccggcga gggggccgcg agcggcttca cgatcgccgg
     5821 gaacaaggtc ctcgtctcca agagcggcat cggccagggg gagcgggcgc atctcacgtc
     5881 gccgccagac atcgctgcct cgatcggcgg cccggcgccg cccagggtca agtggtggaa
     5941 ggagctgaag tagcccttcg ctctctccga gcttctcccg gaaaggggcg ggtcctcccg
     6001 cccctttcct ttttccgctg ggctaggatt cccggagacg gagggaacgc cgtggtcctc
     6061 gagatgatga agaaggggtt ccgcgccgac tcggccctcg tggcgcagct gaaggctcac
     6121 cagtggaccg gcgacgccca gaagaaggag ctcgcggaga agttcgcggc gctccagggg
     6181 ctcgagctcg acgacgtggc ctggacggcc gccgacccga cgcccgagct ccgggcggcg
     6241 ggcctcgcga tcctgcgccg ccggggcgtc gacacggccg cgctcgacgc ggtcgtggcg
     6301 atggggcgga cccgctccga ggccgcgcgg aggggcgtcc agcgcttcct ccgcgacctc
     6361 gcgcagggga cggacctcgg cgcgttcctc tcgcagctcg ccgaccggga ggacacgttc
     6421 gcgaagctga cggcgctcga gatggcgcac gacctgccgc ccgaccgcgc cttcacgatc
     6481 tgccggcggg tgatggtcgg gagcggcgac ccggtcctcc gggcccgcgc cctccgcgcg
     6541 gtcgccgact cgcaggagat ttccgtgacg ccccccgcgc gcgccctcgc gctcggcgcg
     6601 ctgaaggacg acgacgagga gacgcgggtc caggcgctcg cgatcctcga gcgctccccc
     6661 gacgaggcgc tcgtctcgca cctcctcgac ctggcccgct ccggcggagc cgggcgggtc
     6721 gtcgtcgccg cgctcggcgc gctcggccgt ctcctgccga cggcgaaggg agaccatacc
     6781 cgcgagatcc tgacgctcct ggccgacggg cacgagctcg tccgcaaggg ggcgctccag
     6841 ctcgtctcgc gcctcccggc cgacgtcctg gcgccgaagt tcctccaggc gttcgagggg
     6901 acgttcgtct ggatccgcga ccgcgcgctc gagacggtcg tgcagggaaa cccggggttc
     6961 gtcccggcgc tcctgaagct ggccttctcg cccgacccga agctcgccgg gcccgcgcgc
     7021 gagctgtcgc tcctcgtgga cgacccgcgc gccgtgccgg tctggatgca gctcctcgac
     7081 gaccccgact ggtgggtgcg ggcccgcgcc ctcgagaacg tcggccgcca cgggaagggg
     7141 gacgagatcc tcgcgcggct cctcggcctc ctgaaggacc cggaggcgag cctcgccgcg
     7201 gccggcgcgc tcggcgagat gggggacccg cgggcggcgg ggccgctctt cgagctcttc
     7261 aaggcctcgc gcgagcgccc ggacgaccag ctcgagatcc tcgacgcgct ctcgcacctg
     7321 gcgccgaagc agccgaaggt ggtcgacgtc ctcgcgagca tctcgcagct cgagggggtc
     7381 gacctgaacg tccgcgacaa ggcgcggcgg ctcgtcggca ggctctcggg cgacgcggcg
     7441 cgcgacgcgc tcccccgcgt cttcgcgcag cacgcggcgg tggacgtgaa ggcgatcgcg
     7501 accccgacgc tccaggactt cctcgccgac acggtctcga agggggcgtc cgacttccac
     7561 ctggcgaccg gcttcgtgcc gcatcagcgg attcacgggc ggctcgtgcc gatgggaacg
     7621 ccgctgatga cccccgaggt gacggaaggg ctgatccggg ccctcctgac gcccgaggag
     7681 tgggacgagc tgctcgcccg ctacaacctc gacgtctgca tcaaggtgcc cggcctcggg
     7741 cgtttccgcg cgaacttctt ctcgcagcgg gccggctacg acgcctgctt ccgggtgatc
     7801 ccgagctcgg tgccgacgct ctccgacgtc ggcctccccg agtcggcctg ggagctcgcc
     7861 cgcttcccgc acggcctcgt cctcgtgacc gggcccgccg gctgcggcaa gtcgacgacc
     7921 ctcgcggcgc tcctgaacct cgtgaacgag acgcgaaggg ggcacatcat cacgatcgag
     7981 gacccggtgg agttcatcca cccgaacaag gagtgcctcg tgaaccagcg gcaggtgccg
     8041 caccacacgg agtcgttcgc gcgggcgctc cgggccgcgc tccgcgagga ccccgacgtc
     8101 atcctcgtcg gcgagatgcg cgacatcgag acgatcgccc tcgcgatctc ggcggccgag
     8161 acggggcacc tcgtcttcgg gacgctctcg acgacgacgg cgcccgcgac ggtggaccgg
     8221 atcatcaacg ccttcccggc cgggcagcag gggcagatcc ggtcgatggt ctccgagtcg
     8281 ctgaaggcgg tgatctcgca gacgctcctg ccgcgccgcg acggcatcgg ccggatcgcc
     8341 gcgttcgaga tcctccgcgg cacgtcggcg gtgggcgcga tgatccgcga gtcgaagacc
     8401 tatcagctcc ccgggatcct gcagaccggg cagctctccg ggatgaacac gatggaccag
     8461 gcgctcctga agctggccga ggagcggaag gtcgacgcgg aggccgcgat ggaccgcgcg
     8521 cagaggaagg agccgttcga gaagctcgtc gccgaggaaa ggctggggcc gtcgtgagcg
     8581 ccgcccctcc gcagacccgc gcccggatcg actcgctcct ccgggtcgtc ctcgagaaga
     8641 aggcgtccga catccacctc acctgcgggt ccccgccgat gctccgcatc gacggcgagc
     8701 tgacgcggat gaagtggcgg tcgatccgcg aggaggactt cgagaatctc ctccgcccga
     8761 tcgcccccgg ctgggtgtgg gaggagttca cgtcgaccgg cgacggcgac ttcgcctacg
     8821 cgatgggcga caaggcccgc ttccgcgtga acctcttccg gcaggagcac ggctcgggag
     8881 ccgtcctccg gatgatccct ccccgcgtcc tgacgatcga ggacctgggg ctcccggagc
     8941 aggtcgcctc cgtcggccgg acgcagcgcg gcctcgtcct gatcacgggg ccgaccggct
     9001 cgggcaagtc gacgacgctc gcggcgctca tcgaccggat caaccggacg ctgccgtacc
     9061 acatcgtcac gatcgaggac ccggtggagt tcgtccacgt ccccgcgaag tcgatcatca
     9121 cgcaccggga ggtgggcgag aacacgccga gcttcacgac ggccctgaag gcggcgctgc
     9181 gcgaggaccc cgacgcgatc ctcgtcggcg agctgcgcga catcgagacg atgcggatgg
     9241 cgctccacgc ggccgagacg ggcctcctcg tcttcggcac gctccacacg aactcggccg
     9301 cgaaggcgat cgaccggctg atcgacggct tcccctccga cgagcaggag caggtgcgga
     9361 tcgtcctctc cgaggtgctg aaggcggtcg tcgcgcagat cctgatgcgc aagaaggggg
     9421 gcgggcgggt gccggcgttc gaggtcgtcc gcggctcggc ggcgctcgcg aacgcgatcc
     9481 gcgaggggaa gacggggacg atcgcctcgc tcatccagac ggggcgctcg catggaatga
     9541 tctcgctcga ccagtcgctg gccgacctcg tcgtggccga cctcgtcgac gcggacgaag
     9601 cgagcgagta cgccgtggac cgcgactact tccgctccct cgtcgacaag aagaggaacc
     9661 cggaggcgcc ggcttaggag ccctcgctca gggccgcgtc ggcgccggcg gagggagcgc
     9721 gaggcggaaa gcggcgccgc cgagcgccgc cgagcgggag gccgcgacgc tcccgccgtg
     9781 gagcgccgcg atcttcgcga cgagggagag gccgaggccg gtcccccctt ccttcgtcga
     9841 ggcgaagggg acgaagagct tctccgccgc ctcgggggcg acgcccggac cgctgtcctc
     9901 gacgacgagg gcgacgccgc cgccgtccga cggctcgccg cggacggcga cgcggccgcc
     9961 gtcgccggcc gcctccgcgg cgttccggac gaggttgacg agggcccggc ggaggagggc
    10021 cgcgtcggcc tcgacccgcg ggaagtctcc ctcggccgag aacgccgcgc ccgggcaggt
    10081 cgcccgcccc tcggcgagga tctcctcgac gagc
//
LOCUS       DAOTEZ010000115        10104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4701_length_10104_cov_5.412379, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000115 DAOTEZ010000000
VERSION     DAOTEZ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10104
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4701_length_10104_cov_5.412379"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1543
                     /locus_tag="P5164_07825"
     CDS             <1..1543
                     /locus_tag="P5164_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HRY43838.1"
                     /translation="NFSWFDTRRGCIRLKQAGRGGIGTVFRDKGIKALVCRGPRVRGD
                     LNRPADLETLAKVGIKYHREIRENDPHQCMMRRNGTAHIVEIMDAYDLLPTHNFQYGS
                     HPDTHRIASTYWQQRCTQVTADGCWYGCSMACAKGADGLVLKTGPYKGHTVTVDGPEY
                     ENAAGLGSNCGVFDPDWLLELNFYCDTYGICTITWGTLCAFVMECYQRGILNEERTGG
                     LKMTWGNAEADLEMMHQLARGEGFGRIAGQGVHRMKKIFAERGWGDPRLLDDIGMENK
                     GLEYSQYMSKESLAQQGGYALTNKGPQHDEAWLIFMDMVNKQIPTFEDKAEALYYFPL
                     FRTWFGLAGFCKLPWNDVVPADNARQPEAHKVPEHVQNYVDLFTATTGWKITKEELLV
                     QSARVYNFQRVFNIRMGKGLREHDRAPYRSMGPVTREEYESRAERYDRQIVEEMGLDP
                     SGMSLEEKMAAHRRWREERYRKLTDAVYLRRGWTPNGVPTLERLKELGLDALPEVVDV
                     VRQHL"
     gene            1578..2594
                     /locus_tag="P5164_07830"
     CDS             1578..2594
                     /locus_tag="P5164_07830"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY43839.1"
                     /translation="MQGPRSTGSGGRGAGRFVLRALVLLVLVSWGAAARAEAPRLRTV
                     SYLPHWVPQAQFAGFYVAAEKGMYRRHGLEVTVLRGVPEVKSTQALAEGKADFVTTFL
                     STALERWDQGVDLVNVAQLVQRSALLLVAKASSGIRTPADLNGRKVCVWPEFRLQPLA
                     LFRKFGVTPRVIDQRDTLNLFLRGGADAASAMLYNEMHLLRNTGLDEDELVVFRYDAY
                     GLNFPEDGIYCLRSTLERDPAAVRAFVRGSLDGWRWAFEHEEEALDLVMRHVEAAGLP
                     TSRVHHRWMLARMRELMAPDGGRRPLGALEKADFEAVVKEMRAAGLLRKAPDYGTFHV
                     DGTR"
     gene            2575..4668
                     /locus_tag="P5164_07835"
     CDS             2575..4668
                     /locus_tag="P5164_07835"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRY43840.1"
                     /translation="MSTAPARRRWGLAVQLAAVMLAGTTALFALAFGVQYATARRAVL
                     KNAEAGARATVDAHVNRIAAMTSGVEQPPTLLARAVEEDRPDAEELRALLRSALAANP
                     AVYGGTVAFEPRRFSPDRALFAPYYFRRGTALQFEWLDANVDYTVLDWYVLPRELGRP
                     VWSEPYFDEGGGKALMSTYSVPFFETTAGGRRVAGVATADVALDGLVETVAGIRVARS
                     GYAFLISANGVFVAHPDRRRLMRESLFDVAEETDDAELRRIGREMVRGRTGFAPIDNA
                     VLGRDSFLCWAPVPGAGWSLGVVVPKRELLADVARLNQGILLIGGVGIVALWGLAAWV
                     AARTVRPIRPMVELAGTIAEGDLGAALARAPEVERAGASTSAREYQQLSSAFAAMAGS
                     LHGLVSHVRHSGAAVSAAAERIGRSASELGSAAHGQGEAIAQVAEASGGILATTRGLA
                     GTIDAVAQVSGESARRASESRAALADMGSSMRELAETSSGISARLSEIREQAEGIGTI
                     VTTIVAVADRTNLLSLNAAIEAEKAGDAGRGFAVVAREIRRLADQTAIATLDIERRVA
                     GMHGVVSRGARDIDAFVERSRGGIAAVERISGQLEAIIERVEALAPRFADVRSGMDAQ
                     ERGTGQIADAVSALNASVGPTRAALEEFSEVAEELAGAVALLEAEVSRFRVEEPAGEA
                     GPGAAGTGGASSGTA"
     gene            complement(4817..8485)
                     /locus_tag="P5164_07840"
     CDS             complement(4817..8485)
                     /locus_tag="P5164_07840"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRY43841.1"
                     /translation="MKVSRPVAVFARTSALALALGLALPSPTAAAEPLAGRPVPPLGT
                     WTGSCGSGFRLSSMPSAWRVSPGEPVRVFTRFAVDFDALPEEAYAVEVAPDGEKSHRL
                     IGFGSGLLHAVVRDARTGRVVDVLSGARLSAAGSAPAVHTWDGRDQQGRPVPPGLYEI
                     EVRARMVPAWAEPWMREDAAYADLEGWADAAEACTRALRVEVVEGVGGARAALGIAPL
                     ATSCAPAPSSYYASVDVSTPATLRSTLHAVIDDHVRFPYTSSSTDTWDILNDADENPA
                     DPSTVLDLYKNATFADGCSTGCAWNREHTWAKSYGFQTESGSAATPYTDAHHLRATDS
                     GYNSSRSNLPHGECVSGCTEYPTEVNAGFGGSGQPNRGTGGSVSCTTPTPGQVWEVWD
                     HRKGDVARSILYMDLRYEGGTGPLGAEPDLIASDDLSFMYAETTSCGDGYLPVAYHGM
                     LSTLLAWHAADPPDADEIRRNDQVWCYQQNRNPFVDHPEWVDCIYLGSCGGGGALSFG
                     GIVSAVDAAACSAGGVTVTWAAPSAWNDDCTSGCSRGFRVLRDGIALASGGCAAPLAS
                     TATSCTDGTAVAGTTHTYAVEAFNHQSETGTGGKTATATDRTNDGVAPVITAGPTVAS
                     TTTTSFTVTWTTDEPSDGRVEYGLTAAYGAVVSNATLATSHSFTVTGLAAGTTYHFRA
                     GSTDACGTGPRFSVDLVATTAASGGGGTPLAVGGYKLTQANATYVYTLPAGTTVPANG
                     YLVVSRSADKAAFEAFWGVTLGADAAFLNSAGGMPVINGSETYTLADAAGTILDGPTI
                     AMGSSGAQSIRRIDPCQAAGLSSSWTIGAESAATAGSGAGAGCGIGLVINEFSDASGT
                     GSFVYEFLELHYDSAAPADTTAPAASITSPASGATVSGIVEVAASATDAVGVTKVEFL
                     LDGALQATDTTSPYSWSWATAASANGSHTLVAKAYDAAGNVGTSPTVSVTVRNDTTAP
                     VASVTSPASGATVSGTVSVAASATDAVGVTKVEFFLDGVLQATDTASPYSWSWATTGS
                     ANGGHALVAKAYDAAGNVGTSSTVSVTVSNVAGISVGGWRIVQASATLTFTIPAGTVI
                     PANGYLVVGRNATKASFEAFWKTTLGTNVVYLSGGDLFPQVNGSESFTLYNASGTRLD
                     GKTVSMAAAGAQSFQRKDPCLAAGKTTSWTIGSDSNGTPGRGAGAGCAAGVKINEFSD
                     ASGAGNYIFEFVELHSDN"
     gene            complement(8799..>10104)
                     /gene="pckA"
                     /locus_tag="P5164_07845"
     CDS             complement(8799..>10104)
                     /gene="pckA"
                     /locus_tag="P5164_07845"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016873065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HRY43842.1"
                     /translation="YLQGHDLFVQDVYAGADPAYRVPVRIITEKAWHSLFVRNMFVLP
                     QSLEEYRRHVPEFTVIAAPGFQGFPPIDGTNAQTFIALSFDQKVCLIGNTEYAGEIKK
                     SVFTVLNYLLPLDGVLSMHASANVGKEGDSALFFGLSGTGKTTLSADPKRSLIGDDEH
                     GWSDEGVFNIEGGCYAKVIALSAQAEPEIYSTTHRFGTILENVVFDPVTRKIDLDDES
                     ITENTRGSYPLEFIANAVPEKKAGHPKNIIFLTCDAQGVMPPIARLTPDQALYQFISG
                     YTSKIAGTEAGLGKEPELTFSACFGGPFMVHHPYFYADLLKKKIERYGVSCWLLNTGW
                     VGGPYNVGKRISIRYTRALLNAALDGTLHGVEYVKDPVFGFEVPKSCPGIPDSVMDPS
                     SSWPDKAAYQTRYRSLAARFVENFKKFEEGCAPEVIAAGPKL"
BASE COUNT         1416 a         3414 c         3814 g         1460 t
ORIGIN      
        1 gaacttctcg tggttcgaca cccgccgggg ctgcatccgt ctgaagcagg cggggcgggg
       61 cggcatcggg acggtcttcc gcgacaaggg gatcaaggcg ctcgtctgcc gcggcccgcg
      121 cgtccggggc gacctgaacc gcccggcgga cctcgagacg ctcgcgaagg tcgggatcaa
      181 gtaccaccgg gagatccggg agaacgaccc ccaccagtgc atgatgcggc gcaacggcac
      241 ggcgcacatc gtcgagatca tggacgccta cgacctgctg ccgacccaca acttccagta
      301 cggctcgcac cccgacacgc accggatcgc ctcgacctac tggcagcagc gctgcacgca
      361 ggtcacggcg gacggctgct ggtacggctg ctccatggcc tgcgcgaagg gggccgacgg
      421 cctcgtcctg aagacggggc cgtacaaggg gcacacggtc acggtggacg gcccggagta
      481 cgagaacgcg gccggcctcg gctcgaactg cggagtcttc gaccccgact ggctcctcga
      541 gctcaacttc tactgcgaca cctacgggat ctgcacgatc acctggggca cgctctgcgc
      601 cttcgtcatg gagtgctacc agcgcggaat actgaacgag gagcggacgg gcgggctgaa
      661 gatgacctgg gggaacgccg aggcggacct cgagatgatg caccagctgg cccgtggcga
      721 ggggttcggg cggatcgccg gccagggcgt ccacaggatg aagaagatct tcgccgagag
      781 gggctggggc gacccgaggc tcctcgacga catcggcatg gagaacaagg ggctcgagta
      841 ctcccagtac atgtcgaagg agtcgctcgc gcagcagggg ggctacgcgc tgacgaacaa
      901 gggcccgcag cacgacgagg cctggctcat cttcatggac atggtcaaca agcagatccc
      961 gacgttcgag gacaaggcgg aggcgctcta ctacttcccg ctcttccgca cctggttcgg
     1021 cctcgccggg ttctgcaagc tcccctggaa cgacgtcgtc cccgccgaca acgcccggca
     1081 gccggaggcg cacaaggtcc ccgagcacgt ccagaactac gtcgatctct tcaccgccac
     1141 gaccggctgg aagatcacga aggaagagct cctcgtccag tccgcgcggg tctacaactt
     1201 ccagagggtc ttcaacatcc ggatgggcaa ggggctgcgc gagcacgacc gggccccgta
     1261 ccgctcgatg gggccggtga cgcgggagga gtacgagtcc cgcgcggagc gctacgaccg
     1321 gcagatcgtc gaggagatgg gcctcgaccc ctccgggatg tccctcgagg agaagatggc
     1381 ggcccatcgc cggtggcgcg aggagcgcta ccggaagctg acggacgcgg tctacctccg
     1441 tcggggctgg acgccgaacg gcgtaccgac cctggagcgg ctgaaggagc tcggtctcga
     1501 cgccctccca gaggtcgtcg acgtcgtccg gcagcacctg tagccggggc gcccggcgcc
     1561 agaaaggcag gttcgcgatg cagggccctc gttcgacggg aagcggaggt cgcggggcgg
     1621 ggcggttcgt cctccgggcg ctcgtcctcc tcgtcctcgt ctcgtgggga gccgcggccc
     1681 gggccgaggc gccgcggctc cggacggtgt cgtacctgcc tcactgggtg ccgcaggcgc
     1741 agttcgccgg gttctacgtc gcggcggaga aggggatgta ccgacggcac ggcctcgagg
     1801 tgacggtcct gcgcggcgtc cccgaggtga agtcgaccca ggccctggcc gaggggaagg
     1861 cggacttcgt gacgaccttc ctctcgacgg cgctcgagcg ctgggaccag ggcgtcgacc
     1921 tcgtcaacgt cgcccagctc gtccagcgct ccgcccttct cctcgtcgcg aaggcctcct
     1981 ccggcatccg gacgccggcc gacctgaacg gccggaaggt ctgcgtctgg ccggagttcc
     2041 ggctgcagcc gctcgccctc ttccggaaat tcggcgtgac gccgcgcgtc atcgaccagc
     2101 gcgacacgct gaacctcttc ctcaggggcg gcgccgacgc ggcctccgcg atgctttaca
     2161 acgagatgca cctcctccgg aacaccggcc tcgacgagga cgagctcgtc gtcttccgct
     2221 acgacgcgta cggcctgaac ttcccggagg acggcatcta ctgcctccgc tcgaccctcg
     2281 agcgggatcc ggcggcggtc cgggccttcg tccggggctc gctcgacggg tggcgctggg
     2341 ccttcgagca cgaggaggag gccctcgacc tggtgatgcg ccacgtcgag gccgcgggcc
     2401 tgcccacgtc gcgggtccac caccgctgga tgctcgcccg gatgcgcgag ctgatggctc
     2461 ccgacggcgg ccgaaggccg ctcggcgcgc tcgagaaggc cgacttcgag gccgtcgtga
     2521 aggagatgcg ggcggccggc ctgctgcgca aggcgcccga ctacgggacg ttccatgtcg
     2581 acggcacccg ctagacgccg ctggggcctc gcggtccagc tcgcggccgt catgctggcc
     2641 gggacgacgg cgctcttcgc gctcgcgttc ggcgtccagt acgcgacggc ccgccgcgcc
     2701 gtcctgaaga acgccgaggc cggcgcccgc gccaccgtcg acgcgcacgt gaaccggatc
     2761 gcggccatga cctcgggggt cgagcagccg ccgacgctcc tggcgcgggc cgtcgaggag
     2821 gaccggccgg acgcggagga gctgcgcgcg ctccttcgga gcgcgctcgc cgccaacccc
     2881 gccgtctacg gcgggacggt ggcgttcgag ccgcgccggt tctctccgga ccgcgccctc
     2941 ttcgcgccgt actacttccg gcgcggtacg gcgctccagt tcgagtggct cgacgccaac
     3001 gtcgactaca cggtcctcga ctggtacgtc ctcccgcgcg agctgggccg gccggtctgg
     3061 agcgagccgt acttcgacga ggggggcggc aaggccctga tgtcgaccta ctcggtgccg
     3121 ttcttcgaga cgaccgcggg cggccgtagg gtcgccggcg tcgcgacggc cgacgtcgcc
     3181 ctcgacggcc tcgtcgagac cgtcgccggg attcgcgtgg cccgctccgg ctacgccttc
     3241 ctgatctccg cgaacggggt cttcgtcgcc catccggacc ggcgccgcct gatgcgcgag
     3301 agcctcttcg acgtcgccga ggagaccgac gacgcggagc tgcggcggat cggccgggag
     3361 atggtccggg gccggaccgg cttcgccccg atcgacaacg cggtcctcgg cagggactcc
     3421 ttcctctgct gggcgccggt cccgggcgcc ggctggtcgc tcggcgtcgt cgtcccgaag
     3481 cgggagctcc tggccgacgt cgcgcggctg aaccagggca tcctgctgat cgggggggtg
     3541 ggcatcgtcg ccctctgggg cctggcggcg tgggtcgccg cccgcaccgt gcggccgatc
     3601 cgcccgatgg tcgagctggc cgggacgatc gcggaggggg acctgggggc ggcgctcgcg
     3661 cgtgccccgg aggtcgagag ggccggcgcc tccacgagcg cccgcgagta ccagcagctg
     3721 tcgagcgcct tcgcggcgat ggcgggctcg ctgcacgggc tcgtctcgca cgtccgccac
     3781 tccggggccg cggtctcggc ggcggccgag cggatcggac gctcggcgag cgagctggga
     3841 agcgcggcgc atggccaggg cgaggccatc gcccaggtgg cggaggcctc gggcgggatc
     3901 ctggcgacga cccgggggct ggccgggacg atcgacgccg tggcgcaggt ctcgggcgag
     3961 tccgcgaggc gcgcgagcga aagccgggcg gcgctcgccg acatgggcag ctcgatgcgc
     4021 gagctggcgg agacgtcgag cgggatctcc gcgcggctct ccgagatccg ggagcaggcc
     4081 gaggggatcg gcacgatcgt cacgacgatc gtcgcggtcg ccgaccggac gaacctcctc
     4141 tcgttgaacg ccgcgatcga ggccgagaag gccggcgacg cgggccgggg cttcgcggtc
     4201 gtggcgcggg agatccggcg cctcgcggac cagaccgcca tcgccacgct cgacatcgag
     4261 cgccgcgtcg ccgggatgca cggcgtcgtc tcccgcggcg cccgcgacat cgacgccttc
     4321 gtcgagcgct cgcgcggggg gatcgcggcg gtcgagcgga tctccgggca gctcgaggcg
     4381 atcatcgagc gggtcgaggc gctcgcgccg cgcttcgcgg acgtgaggtc cgggatggac
     4441 gcgcaggagc gcggcaccgg ccagatcgcc gacgccgtct ccgcgttgaa cgcgagcgtc
     4501 ggcccgaccc gggcggcgct cgaggagttc tccgaggttg cggaggagct ggcgggcgcc
     4561 gtcgcgctcc tcgaggcgga ggtctcccgc ttccgcgtcg aggagccggc gggcgaggcg
     4621 ggtcccggcg ccgccgggac cggaggggct tcttccggaa cggcctgacg ccggcgcacc
     4681 tcccctgcag gatcgcgtcg ggttcggtct cgagcggcag cccctcccgg cctcgggcga
     4741 gcatcccgcc attccggcag gcgatgcgct cgaaccccgc cgcccggagg acggccgccg
     4801 ctcccctccc cgtgtctcag ttgtccgagt gcagctcgac gaactcgaag atgtagttgc
     4861 cggcgccgct cgcgtcggag aactcgttga tcttcacccc ggccgcgcag ccggcgccgg
     4921 cgccgcggcc gggcgtgccg ttcgagtcgg agccgatcgt ccagctcgtc gtcttcccgg
     4981 cggcgaggca cgggtccttc cgctggaagc tctgtgcccc cgcggcggcc atgctcacgg
     5041 tcttgccgtc caacctcgtg cccgaggcgt tgtagagcgt gaagctctcg ctcccgttga
     5101 cctgcgggaa gaggtccccg ccgctcaggt agacgacgtt cgtcccgagc gtcgtcttcc
     5161 agaacgcttc gaaagaggcc ttcgtcgcgt tccgaccgac gacgaggtag ccgttcgccg
     5221 ggatgaccgt cccggcgggg atcgtgaagg tgagcgtggc gctcgcctgg acgatcctcc
     5281 agccgccgac gctgatgccg gccacgttgc tcacggtgac ggagacggtg gacgaggtgc
     5341 cgacgttgcc ggccgcgtcg tacgccttcg cgacgagcgc atggccgccg tttgcggaac
     5401 cggtcgtcgc ccagctccag gagtacgggg acgccgtgtc ggtcgcctgg aggacgccgt
     5461 cgaggaagaa ctcgactttc gtcactccga cggcatcggt ggcggaggcg gcgacggaga
     5521 cggttccgga gacggtggcg ccggaggcgg gggaggtgac cgaggcgacc ggcgcggtcg
     5581 tgtcgttgcg gacggtgacg gagacggtgg gcgaagtgcc gacgttgccc gcggcgtcgt
     5641 acgccttcgc gacgagcgtg tggctgccgt tcgcggaggc ggccgtcgcc cagctccagg
     5701 aatagggcga cgtcgtgtcg gtggcctgga gcgcgccgtc gaggaggaac tcgaccttcg
     5761 tcacgccgac cgcgtcggtc gcggacgcgg cgacctcgac gatgccggag acggtcgcgc
     5821 ccgaggcggg ggaggtgatc gaggcggcgg gcgcggtcgt gtcggccggg gccgccgagt
     5881 cgtagtggag ctcgaggaac tcgtagacga agctcccggt gccgctcgcg tcgctgaact
     5941 cgttgatcac gagcccgatg ccgcagccgg cgcccgcccc ggagccggcg gtcgcggcgc
     6001 tctcggcgcc gatcgtccag ctcgaggaga ggccggcggc ctggcagggg tcgattcggc
     6061 gaatgctctg ggccccggag gagcccatcg cgatcgtcgg tccgtcgagg atcgtcccgg
     6121 ccgcgtcggc cagcgtgtag gtttcgcttc cgttgatcac gggcatcccg ccggcggagt
     6181 tcaggaaagc ggcgtcggcg ccgagcgtga cgccccagaa ggcctcgaag gcggccttgt
     6241 cggcgctccg tgagacgacg agatatccgt tcgcggggac cgtggtcccg gccgggagcg
     6301 tgtagacgta ggtcgcgttc gcctgagtca gcttgtaccc gccgacggca agcggcgtac
     6361 cgccgccgcc ggaggccgcg gtcgtcgcga cgagatcgac cgagaagcgg gggcctgtcc
     6421 cgcaggcgtc cgtcgagccg gcgcggaagt ggtacgtcgt tccggcggcg agcccggtga
     6481 ccgtgaacga gtgggaggtc gcgagagtcg cgttcgagac gaccgcgccg taggccgccg
     6541 taaggccgta ctcgacgcgg ccgtccgacg gctcgtcggt cgtccaggtg accgtgaacg
     6601 aggtcgtcgt cgtcgaggcg accgtcggcc cggccgtgat caccggcgcg acgccgtcgt
     6661 tcgtccggtc ggtggccgtg gccgtcttcc cgcccgtgcc cgtctcggac tggtggttga
     6721 aggcctcgac ggcgtacgtg tgggtcgtcc ccgcgacggc cgtcccgtcg gtgcaggagg
     6781 tcgcggtcga cgcgagcggc gccgcgcagc cgcccgaggc gagcgcgatg ccatctcgga
     6841 gaacgcggaa gccccggctg cagcctgacg tgcagtcgtc gttccaggcc gaaggagcgg
     6901 cccacgtcac cgtcacgccg cccgccgagc aggccgctgc gtcgacggcc gagacgatcc
     6961 cgccgaacga gagggcgccg cccccgccgc agctgccgag gtagatgcag tcgacccact
     7021 ccgggtggtc gacgaacggg ttgcggttct gctggtagca ccagacctgg tcgttgcggc
     7081 ggatctcgtc ggcgtccggc gggtcggccg cgtgccacgc gaggagggtc gagagcatcc
     7141 cgtggtaggc gaccggcagg tagccgtcgc cgcagctcgt cgtctcggcg tacatgaaag
     7201 acaggtcgtc gctcgcgatc aggtcgggct cggccccgag ggggcccgtg cccccctcgt
     7261 agcggaggtc catgtagagg atcgagcgcg ccacatcgcc cttgcggtgg tcccagacct
     7321 cccagacctg accgggcgtc ggcgtcgtgc aggagaccga gccgcccgtg ccgcggttcg
     7381 gctggccgga gccgccgaag cccgcgttca cctcggtcgg gtactccgtg cagccggaga
     7441 cgcactcgcc gtgcgggagg ttcgagcgcg aggagttgta gcccgagtcc gtcgcgcgaa
     7501 ggtggtgcgc gtccgtgtac ggcgtcgcgg ccgagccgct ctccgtctgg aagccgtacg
     7561 acttggccca ggtgtgctcg cggttccagg cgcagcccgt cgagcagccg tcggcgaatg
     7621 tggcgttctt gtagaggtcg aggacggtgg acggatccgc ggggttctcg tcggcgtcgt
     7681 tcaggatgtc ccacgtgtcc gtcgaggacg acgtgtaggg gaaacggacg tggtcgtcga
     7741 tgacggcgtg gagcgtggag cggagcgtcg cgggcgtcga gacgtcgacg gacgcgtagt
     7801 agctcgacgg ggccggcgcg cagctcgtcg cgaggggcgc gatgccgagc gccgcccgcg
     7861 cgcccccgac tccttccacg acctcgacgc gcagggcgcg ggtgcacgcc tcggccgcgt
     7921 cggcccagcc ttcgaggtct gcgtaggcgg cgtcctcgcg catccacggc tccgcccacg
     7981 cggggaccat ccgggccctc acctcgatct cgtacaggcc gggcgggacg ggccggccct
     8041 gctggtcccg gccgtcccac gtgtggacgg ccggggcgct ccctgccgcg gagagacgcg
     8101 cgccggagag gacgtcgacg acccggccgg tccgcgcgtc gcggacgacc gcgtggagga
     8161 gaccggagcc gaagccgatc agccggtgcg acttctcgcc gtcgggcgcg acctcgaccg
     8221 cgtaggcctc ctcgggcagc gcgtcgaagt cgaccgcgaa tcgcgtgaag acccgcacgg
     8281 gctctccggg cgatacgcgc caggcgctcg gcatggacga aagccggaag ccggacccgc
     8341 aggagccggt ccacgtcccg agcgggggaa cggggcggcc ggcgagtggc tccgccgccg
     8401 ccgtcgggga cgggagggcg aggccgaggg cgagcgcgag agcgctcgtg cgggcgaaga
     8461 cggcgaccgg tcgagacacc ttcatcgagt tcctccgctg gatccgattc gtttctgaga
     8521 gggaagcgca gggcacgcgt cgaaatcgaa gcaggacgtg tgccgtggcg accggctcgc
     8581 cgacgaggcg tatccgggcc ttgcggccgt cgcgttgagg gcgacgagcg gcccattgac
     8641 ccaggcgctc ggaaagcgtg ggtccgttcc cgatccgggg gtctgcaggt gacccgagcg
     8701 gtcgctcccg gggcggtgga gcagcgcggc ccacgagaag gggagcccgc tttcgcgggc
     8761 tcccctcaag tccgggtcgt gtcagatggc ggtgagcgtc agagcttcgg ccccgctgcg
     8821 atgacctcgg gggcgcagcc ttcctcgaac ttcttgaagt tctcgacgaa gcgggcggcg
     8881 agcgagcggt agcgtgtctg gtaggccgcc ttgtcgggcc aggagctgga cgggtccatg
     8941 acggagtcgg ggatgccggg gcagctcttc gggacctcga agccgaagac ggggtccttg
     9001 acgtactcga cgccgtggag cgtgccgtcg agggcggcgt tcaggagcgc gcgggtgtag
     9061 cggatggaga tccgcttgcc gacgttgtac gggccgccga cccagccggt gttcaggagc
     9121 cagcagctga cgccgtagcg ctcgatcttc ttcttgagga ggtcggcgta gaagtagggg
     9181 tggtgaacca tgaacggccc gccgaagcag gcggagaacg tcagctcggg ctccttgccg
     9241 aggcccgcct cggtgccggc gatcttggag gtgtagcccg agatgaactg gtagagcgcc
     9301 tggtcggggg tgaggcgcgc gatcgggggc atgaccccct gcgcgtcgca cgtgaggaag
     9361 atgatgttct tcgggtggcc ggccttcttc tcggggacgg cgttcgcgat gaactcgagc
     9421 gggtaggagc cgcgggtgtt ctcggtgatc gactcgtcgt cgaggtcgat cttccgcgtc
     9481 accgggtcga agacgacgtt ctcgaggatc gtcccgaagc ggtgcgtcgt cgagtagatc
     9541 tccggctcgg cctgcgccga gagcgcgatg accttcgcgt agcagccccc ctcgatgttg
     9601 aagacccctt cgtcgctcca gccgtgctcg tcgtcgccga tgaggctccg cttcgggtcg
     9661 gccgagagcg tggtcttgcc ggtgccggag aggccgaaga agagggcgga gtcgccctcc
     9721 ttgccgacgt tcgcggaggc gtgcatggag aggacgccgt cgagcgggag gaggtagttg
     9781 aggacggtga agaccgactt cttgatctcg cccgcgtact cggtgttgcc gatgaggcag
     9841 accttctggt cgaagctcag ggcgatgaac gtctgcgcgt tcgtcccgtc gatcggcggg
     9901 aagccctgga agcccggcgc cgcgatgacg gtgaactccg ggacgtgccg gcggtactcc
     9961 tcgaggctct gcggcaggac gaacatgttc cgcacgaaga gggagtgcca cgccttctcg
    10021 gtgatgatcc ggaccgggac gcggtaggcc gggtcggcgc cggcgtagac gtcctggacg
    10081 aagaggtcgt gcccctgcag gtag
//
LOCUS       DAOTEZ010000116        10102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4702_length_10102_cov_6.311735, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000116 DAOTEZ010000000
VERSION     DAOTEZ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10102
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4702_length_10102_cov_6.311735"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /locus_tag="P5164_07850"
     CDS             complement(<1..1466)
                     /locus_tag="P5164_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRY43843.1"
                     /translation="MILNPKRLGELPLDARSREIVEKTVAFFEAKGKARLKQDDFDRV
                     WYADFLRFVKEERIFADLLTPPELGGPDARWDTFRNCFVNEVLGFYGLCYWYTWQVSI
                     LGLGPLWMSPNEATRKKTAAALADGGIFAFGLSEKEHGADIYSTSMKLAPRGDAFVAT
                     GTKYYIGNGNEAAIVSVFGKMEGTGEYVFFAADPKRAGYELVRNVVASQSYVAELALE
                     DYPVAADEVLLRGPEAWDASLNTVNVGKYNLGWASIGICEHAFHEALSHAAGRRLYGM
                     SVTDFPHVARMFVDAWARLVAMKVFAFRASDYLRAASLKDRRYLLYNPLVKMKVTTEG
                     EKVIDLLWDVIAAKGFEKDMVFEMAARDIRALPKLEGTVHVNIALIVKFLANWLFRPT
                     SYPEVGRRTEPVHDAYLFAQGPARGLGKIRFHDWAPAFERYARVPNVALFREQLGAFR
                     ELVAAAAPTEDQAKDVDLLLAVGDLFALAVYAHLVLEGA"
     gene            1646..2743
                     /locus_tag="P5164_07855"
     CDS             1646..2743
                     /locus_tag="P5164_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43844.1"
                     /translation="MADPGRGIEQQLEESVRRVLGALERMPVGKIEVVANEVSRAVGQ
                     VVSQVDRASRTPTREERRLEKRLREEDEASLPQAWVMAGAAVVAGLIGLTQPQLFWFF
                     FVGFGFAMGAADIFAKVRRRERRQAEAAQERGAAVTGAATERAAPAPLVTPPPPAQAE
                     RPALAAVSAREARVDGICDRLLSELSGAPPLVRELLRRPEQTVEALRSASRELARRER
                     ELRGMLSEDEGERLVGERAELAARAEAAPDAVVRSRLSAALEALDAQLVHRAELTTAA
                     ARIEAEGTRILYSLESLRAQVLRAFAADAAAADVTRESLKGGLETLRGEIDAVATALE
                     EVHGVGKPPVASTAGGPTAAARAALRQAGTR"
     gene            2758..6060
                     /locus_tag="P5164_07860"
     CDS             2758..6060
                     /locus_tag="P5164_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43845.1"
                     /translation="MTSAAAPPRVGAPGSGRRLPGRPAHGPVRRLGLSLVLLLLPATD
                     LRGDPVDVPGGTASIRRLLDLGERRTESDFFAEVGRVLLSGTNPADPWSKSEKRKAVV
                     SFAEDLEEWKGKQGCPAILSTRPGAWEGTRRALGWLGYRVRGEGPGFEAEPLAEPEAV
                     RRQTFLDVLGQPAAAVLARLAAGEDVRVECASGTAELPFSLAAWRETLGLDEKRLNAR
                     NAFLRFVTDVPASRMMVALHGVDARTREELRAVGGPAGGFAGWKLVYEKALDGFALYP
                     EALEIRNGKIRLPGGAAAESVWAAVVGESPSDLPRFVVRFFGSGSGKAAYVADALRPL
                     PEETARAFVLGATTGGETSVERFERLFSAIGKTGRSYGNTQRDPWDFTQLAGFLRPAA
                     GGEIALPGGAGLWMEALARPALPANEKELSDVLAAAAKRTEGPAEALERVLREETEGV
                     SGPLPASKRFVVVSSLVRDRPVLGEPGTVLLLARGVDRFLGCYAPLEELPLVDAETAR
                     RYLFTLNRLDTNGTDRDAELRAGLFLASVQVLSDLFRSGSISDAGARELFASLLGQPL
                     FATPRTAPAAGMEPYDRWLHGELLAALREAETRFLGAERAEALAWEDEEGPRPAKTAD
                     ELLSAALSGFRPPAALAWRGGRYSYDASEDAVARRRTFARTQDHVPLWVLARAASERE
                     NALAAARAGDVEEARVALGALLESLGAIPAGREADERVAATASGARHVLGTLQAATER
                     NVASLLEAGLPRLDALRAERTLEALAVHVYSGCVLDPGDLTFADSRLVRRHSLSWGPR
                     RGIVVPSPFQPARIETPEEGASLRLAGGWAGIGSPLGLLHAEGLVYDERALLSNDAVR
                     AGLVLPAVLFTPARLDDEALRFVALACRAAEQLPGALAGLPEAQRHDAWSRLARDLVP
                     ASRRNRLAREGAVAVADDLSPSDLLRIGRRLALRTDPSLPDVPAAREAREVWSGRVAR
                     LGEAGAKAFLDELGARAFHWEGRSRLACLDLPSYERLSEYRVPQLFGDRLHDLPVAVA
                     RSVFDAGDPAGVVPLVLRSAIDDLLSRARMAYAFDWRPVAATSATSAAYWPAARDEVI
                     GAALAAGRVRRM"
     gene            6081..7214
                     /locus_tag="P5164_07865"
     CDS             6081..7214
                     /locus_tag="P5164_07865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRY43846.1"
                     /translation="MTRRSLLSLATLLVALAAGPARADDAPSLRIVRPGPDDISLGET
                     AIEVRLTGHRPGDAVEVFVDGRPAGTLHGEPWTLTWSAGNAPRPHRIEAVLRRDGGEA
                     AVSRVRTRGLGFSATADARVVSLSPIVTDSSGSYVRGLVREDFSVFVDGQPQEIETFE
                     SVNSALSVVLVLDQSSSMALKLRNARGAALEFLAALKPGDRAAVLTFASNVVGFTPFS
                     TDRSAARKALEAARVSGETALYDAAAVALRRLRETSGRRAVVLFTDGEDNRSRMSIDQ
                     VVDLARATEASIFTVAQGTAKVRALLRGLDRLASETGGRAWFISSIKSLPDVFREVVA
                     ELENQYFLTFTPADQRPRTWHRVEVKTKKPGLTVRARKSFRID"
     gene            complement(7260..7730)
                     /locus_tag="P5164_07870"
     CDS             complement(7260..7730)
                     /locus_tag="P5164_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRY43847.1"
                     /translation="MRDAPPAIRAGSRSGLVLLAVLVVLLAAPAVLAGEEMDVRKDGS
                     LWARLETGGAVRIDGVVVGEIEGDGTIRVEGRVAGRIEEDGEIREDGTVRTRVEKDGT
                     LRRNGTVIGRIEEGGAIRRHGRIWGEATPCCASFDDLRRLTALLLFFDRSFFVR"
     gene            7866..>10102
                     /locus_tag="P5164_07875"
     CDS             7866..>10102
                     /locus_tag="P5164_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRY43848.1"
                     /translation="MAEERSYGDRPATVTVGLVDGRTFVGRLGRFRPADSDLLLVVRA
                     RDVAGIASETPQRVASEQVAYVAVHRSGTPVAGPSGETVSLDVHVAGGQTFAVSVETA
                     RKDDRLGFWGVPASASSAFGEVWFYAHGVNAREEKVTLGALLVEHGALGGDGLARGLE
                     KQAEAREVPLGQILVEQKKVGEEEVAAAAATQKDAISKGKRLRLGEVLVEAGLAREED
                     IEAALAEQKKRRGKRIGQVLVEMGLVSEQEIARALSRKFNVPFVDLDAVEIDPKAVSL
                     VPMDLIQRYSVLPIRADETSVTIALSDPLAYEAIDLLRFTLGKRIVENVATPSQLARL
                     IEPLQVVKEDAPLTDQMEEILSSIEIEPSSEGGPDARDLSVARDDESGVAKLVNQIIV
                     DAYNRGVSDIHIEPDGDERETTVRFRIDGVCETYRRFQPALRSRLVARLKIMANLDIT
                     ERRKPQDGKIRLRMKDKPIELRVATIPVVSRDEDVVMRILAAGEPIPMEKLLLSPRNF
                     SELKRVVSVPYGLVLAVGPTGSGKTTTLHSLVGSINTPVRKIWTAEDPVEITQKGLRQ
                     VQVQPRIGFTFATALRAFLRADPDVIMVGEMRDHETASIGIEASLTGHLVFSTLHTNS
                     APETVTRLVDMGLDPFTFSDALLAVLAQRLARRLCPACKAPYEATPAEREEVAGLLGR
                     ELLEKALEGRPLTLHRAAGCPKCEGRGYKGRLGVHELLVNDERLRLAIQRKSSVDEVR
                     AMAVEG"
BASE COUNT         1350 a         3723 c         3647 g         1382 t
ORIGIN      
        1 gccccctcga ggacgaggtg ggcgtagacg gcgagggcga agaggtcgcc gacggcgagg
       61 aggaggtcga cgtccttcgc ctggtcctcg gtcggcgcgg cggcggcgac gagctcgcgg
      121 aacgcgccga gctgctcgcg gaagagggcg acgttcggga cgcgggcgta cctctcgaag
      181 gccggcgccc agtcgtggaa gcggatcttc ccgagacctc gcgccggccc ctgcgcgaag
      241 aggtaggcgt cgtgcaccgg ctccgtccgc cgtcccactt cggggtagga cgtcgggcgg
      301 aagagccagt tcgcgaggaa cttcacgatg agcgcgatgt tgacgtggac cgtcccttcg
      361 agcttcggca gggcgcggat gtcgcgcgcg gccatctcga agaccatgtc cttctcgaag
      421 cccttcgccg cgatgacgtc ccagaggagg tcgatcacct tctctccctc ggtcgtcacc
      481 ttcatcttca cgagcgggtt gtagaggagg tagcgacggt ccttgagcga ggcggcccgc
      541 aggtagtccg aggcgcggaa cgcgaagacc ttcatcgcga cgaggcgcgc ccaggcgtcg
      601 acgaacatcc gcgcgacgtg cgggaagtcg gtcaccgaca tcccgtagag gcggcggccg
      661 gccgcgtggc tcagcgcctc gtggaacgcg tgctcgcaga tcccgatcga ggcccagccg
      721 aggttgtact tcccgacgtt gacggtgttg agcgaggcgt cccacgcctc ggggccgcgg
      781 aggaggacct cgtccgcggc gacggggtag tcctcgaggg cgagctcggc gacgtacgac
      841 tggctcgcga cgacgttcct gacgagctcg taaccggccc gcttcgggtc ggccgcgaag
      901 aacacgtact cgcccgtccc ctccatcttc ccgaagacgg agacgatcgc agcctcgttg
      961 ccgttcccga tgtagtactt cgtgcccgtc gcgacgaacg catcgccccg cggcgcgagc
     1021 ttcatggagg tcgaatagat gtcggcgccg tgctccttct cggagaggcc gaaggcgaag
     1081 atgccgccgt cggcgagcgc cgcggccgtc ttcttccgcg tcgcctcgtt cgggctcatc
     1141 cagagcgggc cgaggccgag gatcgagacc tgccacgtgt accagtagca gaggccgtag
     1201 aagccgagga cctcgttcac gaagcagttg cggaacgtgt cccagcgggc gtccggccct
     1261 ccgagctccg gcggcgtcag gaggtcggcg aagatccgct cctccttcac gaagcggagg
     1321 aagtcggcgt accagacccg gtcgaagtcg tcctgcttca gccgcgcctt ccccttcgcc
     1381 tcgaagaagg cgaccgtctt ctccacgatc tcccgcgagc gcgcgtcgag tgggagctcc
     1441 ccgagcctct tcgggttcag gatcatggcg tcctccgtcc ctccagccta ccccacgagg
     1501 gaggggtcag gtctaccatc tacactctgt gcgccgttcc cggccggccc ggcgcatcgg
     1561 gccgggcgaa cggagcgagc ccggccggcg gggcgcctga acccttcccg ctccgcggcg
     1621 tataacccgc tggcgggagg acgagatggc cgatccgggc cggggcatcg agcagcagct
     1681 ggaggagagc gttcgccggg tgctcggcgc gctcgagcgg atgcccgtcg ggaagatcga
     1741 ggtcgtcgcg aacgaggtct cccgcgccgt cggacaggtc gtctcccagg tcgatcgcgc
     1801 gagccggacc ccgacgcggg aggagagacg cctcgagaag aggctgcggg aggaggacga
     1861 ggcgagcctg ccccaggcgt gggtgatggc cggggccgcc gtcgtcgccg ggctcatcgg
     1921 cctcacccag ccgcagctct tctggttctt cttcgtcggc ttcggcttcg cgatgggcgc
     1981 ggccgacatc ttcgcgaagg tccgtcgccg ggagaggcgg caggcggagg cggcgcagga
     2041 gcgcggcgcc gccgtcaccg gggcggccac cgagcgggcc gccccggcgc cgctcgtcac
     2101 accgccgcct ccggcgcaag ccgagaggcc cgccctcgcc gcggtctcgg cccgggaggc
     2161 gcgcgtcgac ggcatctgcg atcggctcct ttccgagctt tcgggagcgc cgccgctcgt
     2221 gcgggagctc ctgcgccggc ccgagcagac cgtcgaggcg ctccgttcgg cgtcgaggga
     2281 gctcgcccgc cgcgagcgcg agctgcgcgg gatgctctct gaggacgagg gagagcggct
     2341 cgtgggcgag cgggcggagc tggcggcccg ggcggaggcc gcgcccgacg ccgtcgtgag
     2401 gagccgtctc tcggcggccc tcgaggcgct cgacgcccag ctcgtccacc gggccgagct
     2461 gacgacggcc gcggcacgca tcgaagccga ggggacgcgg atcctctact cgctcgagag
     2521 cctgcgcgca caggtccttc gcgcgttcgc cgccgatgcc gccgcggccg acgtcacgcg
     2581 agagagcctg aaagggggcc tcgagacgct ccgcggcgag atcgacgcgg tggcgaccgc
     2641 gctcgaggag gtccacgggg tcggaaagcc gccggtcgcc tcgaccgcag ggggaccgac
     2701 cgccgcggcg cgcgccgccc ttcggcaggc gggaacgcgc tgagggcctc gccgccgatg
     2761 acctccgccg ccgccccgcc ccgggtcgga gcccccggct ccggccgacg tctcccgggc
     2821 cgtccggcgc acggaccggt ccggcgtctc ggcctgtcgc tcgtcctgct cctcctcccg
     2881 gcaaccgacc tgcgcggaga cccggtcgac gtccccggcg ggaccgcgag catccgtcgt
     2941 cttctcgacc tcggcgagcg gcggaccgaa tcggatttct tcgccgaggt ggggcgggtc
     3001 ctcctctccg ggacgaaccc ggccgacccg tggagcaaga gtgagaagcg gaaggcggtc
     3061 gtctccttcg cggaagacct cgaggagtgg aaggggaagc agggctgtcc ggcgatcctc
     3121 tcgacgaggc cgggcgcgtg ggaggggacc cggcgggcgc tcggctggct cggctatcgg
     3181 gtccgcggag agggcccggg attcgaggcg gagcccctcg ccgagcccga ggcggtgcgc
     3241 cgccagacct tcctcgacgt cctggggcag cccgccgcag cggtcctcgc gcgcctcgcg
     3301 gccggggagg acgtccgcgt ggagtgcgcg tccggcacgg ccgagctccc gttctcgctc
     3361 gcggcgtggc gcgagacgct cggcctcgac gagaagcgtc tgaacgcccg gaacgccttc
     3421 ctccgcttcg tcaccgacgt cccggcgtcc cggatgatgg tggccctgca cggggtcgac
     3481 gcgaggaccc gggaggagct ccgggcggtc ggcgggccgg ccgggggatt cgccggctgg
     3541 aagctcgtct acgagaaggc gctcgacggc ttcgccctct accccgaggc gctcgagatc
     3601 cggaacggga agatccggct gccgggaggc gcggccgcgg aatcggtctg ggcggcggtc
     3661 gtcggagagt cgccgtccga ccttccccgg ttcgtcgtga ggttcttcgg ctccggctcg
     3721 ggcaaggccg cgtacgtggc cgatgcgctc cggccgctgc ccgaggaaac cgcgcgagcc
     3781 ttcgtcctcg gcgcgacgac cggtggcgag acgtccgtcg agcggttcga gcgcctcttc
     3841 tccgcgatcg ggaagaccgg ccggagctac gggaacaccc agcgcgaccc ctgggacttc
     3901 acccagctcg ccggcttcct ccggccggcg gccggcggag agatcgccct cccgggcgga
     3961 gccggactct ggatggaggc cctcgcgcgc cccgctctgc ccgcgaacga gaaggagctc
     4021 tcggacgtcc tcgccgctgc ggccaagcgg accgagggac cggccgaggc gctggagcgc
     4081 gtcctccggg aggagaccga gggggtctcc ggtccgctgc cggcgtcgaa gcgattcgtc
     4141 gtcgtctcga gcctcgtccg cgaccggccc gttctcggcg agcccggcac cgtcctcctc
     4201 ctcgcccgcg gcgtcgaccg cttcctcggc tgctacgcgc cgctcgagga gcttccgctc
     4261 gtcgacgcgg agaccgcccg gcggtacctc ttcacgctga accgcctcga cacgaacggc
     4321 accgatcgcg acgccgagct ccgcgccggc cttttcctcg cctcggtcca ggtgctctcg
     4381 gacctcttcc ggagcgggtc gatctccgac gcgggtgcgc gcgagctgtt cgcgtccctc
     4441 ctcggtcagc cgctgttcgc gacgccccgg accgctcccg ctgcgggtat ggagccctac
     4501 gatcgctggc ttcacggcga gctcctcgcc gcgctccgcg aggcggagac ccggttcctc
     4561 ggcgcggaac gcgcggaggc gctcgcctgg gaggacgagg aggggccgcg accggcgaag
     4621 acggccgacg agctcctctc cgcggccctt tccggcttcc gcccgccggc cgccctcgcc
     4681 tggaggggag gaaggtactc gtacgacgcc tcggaagacg ccgtggcgcg acgtcggacg
     4741 ttcgcccgga cccaggacca cgtcccgctc tgggtcctcg cccgggcagc ctccgagcgc
     4801 gagaacgcgc tcgccgccgc gcgcgcggga gacgtcgaag aggctcgcgt cgccctcggc
     4861 gcgctcctcg agagcctcgg ggccatcccg gccgggcggg aggccgacga gagggtggcg
     4921 gcgacggcgt ccggcgcccg ccacgtcctc ggcacgctcc aggccgcgac ggagaggaac
     4981 gtcgcgtcgc tcctcgaagc cggtcttccg cgcctcgacg cgctgcgagc cgaacggacg
     5041 ctcgaggcgc tcgccgtcca cgtctactcc ggatgcgtcc tcgacccggg cgatctcacg
     5101 ttcgccgact cccggctcgt ccggaggcat tccctctcgt ggggtccccg caggggcatc
     5161 gtggttccct ccccgttcca gccggcgcgg atcgagacgc ccgaagaggg agcgtccctg
     5221 aggctggcag ggggctgggc gggaatcgga agccccctcg ggctcctgca cgcggagggg
     5281 ctcgtctatg acgagagagc cctcctctcg aacgacgccg tccgcgccgg gctggtcctt
     5341 cccgccgttc tcttcacgcc ggcgcgcctc gacgacgagg cccttcgctt cgtcgcgctg
     5401 gcctgccggg ccgccgagca gcttccgggc gccctcgccg gcctccccga agcgcagcgg
     5461 cacgatgcct ggagccggct cgcccgcgac ctcgtcccgg cctcccggcg aaacaggctc
     5521 gcccgggagg gggcggttgc ggtggccgac gacctctctc cgtcggacct ccttcggatc
     5581 gggcggcggc tcgcgcttcg gaccgacccg tcgctgccgg acgtcccggc ggcgcgcgag
     5641 gcgcgcgagg tctggtcggg ccgtgtcgcg cggctcggcg aggcgggggc gaaggcgttc
     5701 ctcgacgagc tcggagcgag agccttccac tgggaaggcc ggagccgcct ggcctgcctc
     5761 gacctgccgt cttacgagcg gctctcggaa taccgcgtgc cgcagctctt cggcgaccgc
     5821 ctccacgacc tcccggtcgc cgtcgcgagg tcggtcttcg acgcgggcga cccggcgggc
     5881 gtcgtccctc tcgtcctccg gtccgcgatc gacgacctcc tcagccgggc ccgaatggcg
     5941 tacgccttcg actggcggcc cgtcgccgcg acctccgcga cctccgcggc ctactggccg
     6001 gcggcgaggg acgaggtcat cggcgccgcg ctcgcggccg gccgggtccg gcggatgtag
     6061 attcgagacg caggacctcg atgacgcgtc gctcgctcct ttcgctcgcc accctgctcg
     6121 tcgcgctcgc ggcgggcccg gcccgcgcgg acgacgcgcc ctccctgcgg atcgtgaggc
     6181 cggggcccga cgacatctcg ctcggtgaga cggcgatcga ggtgcgtctc acggggcacc
     6241 ggcccggcga cgccgtcgag gtcttcgtcg acggccggcc cgccggcacg ctccacggcg
     6301 agccgtggac gctcacctgg agcgcgggga acgccccgcg cccgcaccgc atcgaggccg
     6361 tcctccggcg cgacggcggc gaagccgcgg tctcgcgtgt gaggacgcgc gggctcgggt
     6421 tctccgcgac cgccgacgcc cgggtcgtct ccctctcgcc gatcgtcacc gattcgagcg
     6481 ggagctacgt ccgtggcctc gtgcgcgagg acttctccgt cttcgtcgac ggccagccgc
     6541 aggagatcga gaccttcgag tcggtgaact ccgccctctc ggtcgtcctc gtcctcgacc
     6601 agtcgagctc catggcgctc aagctccgga acgcgcgcgg cgcggcgctc gagttcctcg
     6661 cggccctgaa gccgggcgac cgggccgccg tcctcacctt cgcgtcgaac gtcgtcggct
     6721 tcaccccgtt ctcgacggac cgctcggcgg cgcggaaggc gctcgaggcc gcgcgcgtga
     6781 gcggggagac cgccctctac gacgccgccg ccgtggcgct ccggcgtctc cgcgagacga
     6841 gcgggcggcg cgccgtggtc ctcttcaccg acggcgagga caaccggagc cggatgtcga
     6901 tcgaccaggt cgtcgacctc gcgcgcgcca cggaggcgag catcttcacg gtggcccagg
     6961 ggacggcgaa ggtccgggcg ctcctcaggg gcctcgaccg gctcgcctcg gagacgggcg
     7021 gacgagcctg gttcatctcc tcgatcaagt cccttccgga cgtctttcgc gaggtcgtcg
     7081 ccgagctcga gaaccagtac ttcctgacct tcactccggc cgaccagcgg ccgcggacct
     7141 ggcaccgggt cgaggtgaag acgaagaagc cgggcctgac ggtccgcgcg cggaagtcgt
     7201 tccggatcga ctgacgggcg gggagagcca cgccgcgggc gagccgcggg aggcggccgt
     7261 cagcggacga agaagctgcg gtcgaagaag aggaggagcg ccgtcagccg gcgcaggtcg
     7321 tcgaacgagg cgcagcaggg ggtcgcctcg ccccagatcc tcccgtggcg ccggatggcg
     7381 ccgccctcct cgatccgccc gatgaccgtc ccgttccggc ggagcgtccc gtccttctcg
     7441 acgcgcgtcc ggaccgtccc gtcctcgcgg atctccccgt cctcctcgat gcggccggcg
     7501 acgcggccct cgacgcggat ggtcccgtcg ccctcgatct cgccgacgac gacgccgtcg
     7561 atccgaaccg ctccgcccgt ctcgagccgg gcccagaggc tcccgtcctt cctcacgtcc
     7621 atctcctcgc cggcgaggac ggcgggcgcg gcgaggagca cgacgaggac cgcgaggaga
     7681 acgaggccgc tcctcgagcc ggcgcggatc gcgggcgggg cgtctctcat cggagcctcc
     7741 cggcaggagg gcctgccgtc ccgattctgg gaatcccggc ggccgaggtc aatcggcgca
     7801 ccgtcggccc cgcccgcctc ctgctactct cgtccctcct ccgggcacgc cgcgtgggag
     7861 ggaccatggc cgaggaacgc agctacggag accgtcccgc caccgtgacg gtcgggctcg
     7921 tcgacggacg gacgttcgtg gggcgcctcg ggcgcttccg cccggccgac tccgacctcc
     7981 tcctcgtcgt ccgggcgcgg gacgtcgccg ggatcgcctc cgagacgccc cagcgcgtcg
     8041 cctccgagca ggtcgcctac gtcgccgtcc accggagcgg gacgcccgtg gcgggccctt
     8101 cgggcgagac ggtcagcctc gacgtccacg tcgccggggg ccagaccttc gcggtctcgg
     8161 tcgagacggc ccgcaaggac gaccggctcg gcttctgggg agtcccggcg agcgcctcga
     8221 gcgccttcgg cgaggtctgg ttctacgcgc acggcgtcaa cgcgcgggag gagaaggtca
     8281 cgctcggagc cctcctcgtg gagcacggcg ccctcggggg cgacggcctc gcgcgcggcc
     8341 tcgagaagca ggccgaggcg cgggaggtcc cgctcgggca gatcctcgtc gagcagaaga
     8401 aggtcggcga ggaggaggtc gcggccgccg cggcgacgca gaaggacgcg atttcgaagg
     8461 ggaagcgcct ccgcctcgga gaggtgctcg tcgaggccgg gctggcccgc gaggaggaca
     8521 tcgaggccgc cctcgccgag cagaagaagc ggcgcgggaa gcggatcggg caggtgctcg
     8581 tcgagatggg cctcgtcagc gagcaggaga tcgcgcgggc gctctcgagg aagttcaacg
     8641 tccccttcgt cgacctcgac gccgtggaga tcgacccgaa ggccgtctcc ctcgtcccga
     8701 tggacctcat ccagcgctac agcgtcctgc cgatccgggc cgacgagacg agcgtgacga
     8761 tcgcgctctc ggacccgctc gcctacgagg cgatcgacct cctccgattc acgctcggga
     8821 agcggatcgt ggagaacgtg gcgaccccgt cccagctcgc ccggctcatc gagccgctcc
     8881 aggtcgtcaa ggaggacgcg cccctcaccg accagatgga ggagatcctc tcctcgatcg
     8941 agatcgagcc ctcctccgaa gggggcccgg acgcgcgcga cctctcggtc gcccgcgacg
     9001 acgagagcgg cgtcgcgaag ctcgtgaacc agatcatcgt cgacgcctac aacaggggcg
     9061 tctccgacat ccacatcgag ccggacgggg acgagcggga gacgacggtc cgcttccgga
     9121 tcgacggcgt ctgcgagacc taccggaggt tccagccggc gctccggtcg cgcctcgtcg
     9181 cgcggctgaa gatcatggcg aacctcgaca tcaccgagag gcgcaagccc caggacggga
     9241 agatccggct ccggatgaag gacaagccga tcgagctgcg cgtggcgacg atcccggtcg
     9301 tcagccggga cgaggacgtc gtcatgcgga tcctcgccgc cggcgagccg atcccgatgg
     9361 agaagctcct cctctcgccg cggaacttct cggagctgaa gcgggtcgtc tcggtcccct
     9421 acggcctcgt cctcgcggtc ggcccgaccg gctcgggcaa gacgacgacg ctccactcgc
     9481 tcgtcggctc gatcaacacg ccggtgagga agatctggac ggcggaggac ccggtcgaga
     9541 tcacgcagaa ggggctccgg caggtgcagg tgcagccccg gatcggcttc acgttcgcca
     9601 cggcgctccg ggcgttcctc cgggccgacc ccgacgtcat catggtcggc gagatgcgcg
     9661 accacgagac ggcctcgatc ggcatcgagg cctcgctcac cgggcacctc gtcttctcga
     9721 ccctccacac gaacagcgcc cccgagacgg tgacccgcct cgtcgacatg ggcctcgacc
     9781 cgttcacgtt ctccgacgcc ctcctcgccg tcctcgccca gcgcctcgcg aggcgcctct
     9841 gccccgcctg caaggcgccg tacgaggcga cacccgccga gcgggaagag gtggccggcc
     9901 tcctcggccg agagctcctc gagaaggcgc tcgagggccg ccccctgacg ctccatcgcg
     9961 ccgccggctg cccgaagtgc gaggggcgcg gctacaaggg gcgcctcggc gtccacgagc
    10021 tgctcgtgaa cgacgaacgc ctgcgcctcg cgatccagcg gaagtcgagc gtcgacgagg
    10081 tccgcgcgat ggcggtggag gg
//
LOCUS       DAOTEZ010000117        10097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4707_length_10097_cov_6.086337, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000117 DAOTEZ010000000
VERSION     DAOTEZ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10097
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4707_length_10097_cov_6.086337"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="P5164_07880"
     CDS             complement(<1..616)
                     /locus_tag="P5164_07880"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRY43849.1"
                     /translation="MKPNTLSVNREVPATVHNGWPMLVFALLLLAADIGLFVWSIAAG
                     VQAKGDPYVLPFVVSLLGLPAWIVFVTGFFTLQPNEARVLVLFGAYKGTVREAGFHWG
                     NPFYSNGSGAAAAAAAAAASAEGHGAKPAALAAQAAQAAGRRSSKRNRISLRARTLNG
                     QRLKVNDKGGNPIEIAAVVIWRVQDTAMAMFDVDDYEQYVETQSE"
     gene            776..1693
                     /locus_tag="P5164_07885"
     CDS             776..1693
                     /locus_tag="P5164_07885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRY43850.1"
                     /translation="MRRAGAARREVDTADRSPGGERRRREKGPAAGRAAASGAALPTT
                     PRCDSCDVAKTYAAEKARLAVWLAHELRTPLNCVLGYTELLCDQLEAPGHATLLADAL
                     RVRDLALHQLTLLDEVLDLARLEHGSLALRVETIDATRVLEGIAAASLPIVRRGGNRV
                     LLTTEGPTWVLADETRLRQVLLNLVGNAAKFTRDGLVTLGATEANGSVLLHVSDTGPG
                     MAPGVVERLFRPFQQASAAVAAAHGGSGLGLAISKELCERMGGSISLRTAEGEGTTFT
                     VTLRAAPKPRPAAGSGGRAKTEVRESASA"
     gene            complement(1671..3155)
                     /locus_tag="P5164_07890"
     CDS             complement(1671..3155)
                     /locus_tag="P5164_07890"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRY43851.1"
                     /translation="MNRLAKNAALLAAFAATTALGQAIPDRPEKLTYPAISWEVPKAK
                     DAKVVLKNKVPAYLVADPSGVPLVRVSVWWRGGAYLDPAGKEGLASLFGSQLAVGGTQ
                     KTDAAKLEDRLEALAATLSSSCGPTSGGIHIRVQEKDLAEGLALLMQALTQPAFAQDR
                     LDLAKKNARQGLERRNDAVTSIAQLQMPALLFGEGFFATERTTAASLDAITREDLLAL
                     HASLLHPANLVVEVSGKLERRAMTDLLNRTVGAIKAGPAAKPSPKVPLPEFARKPGLY
                     VTDKDAPQAMLQWAFPGMRRSDPDWYPAMVMNHVLGGGGFAARLMKKIRSDEGLTYGV
                     RTQLGEGPYWRGDLTGGLQTKNATVAYALRLALAEMQRLKDEPLTEAELASIKDGLVE
                     AFPAQWGSRQAVANRFADENLNGWPEDWWLDYREKVKAVTAADVQKAARKLLDFEKMV
                     VLAVGKASEIEAGDADHPGALKDAAKLPLARLPLRDPLTLKPIP"
     gene            complement(3152..4843)
                     /locus_tag="P5164_07895"
     CDS             complement(3152..4843)
                     /locus_tag="P5164_07895"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRY43852.1"
                     /translation="MTVFRGRAPAALLAAAILAAPLSAQELNVVERTLPNGMKLLMVP
                     RHDQPTISCGWLARVGSANERPGITGLAHLFEHMMFKGTTTIGTRDAARDAELNRAQD
                     EVQAQIREEMTLLREKQRRGEIADMMSPAARTPRLQELLSKFDALVKEQRALIVKDEM
                     DKVYQENGATGLNASTSQDRTFYIVGLPSNKLELWMWLESDRLGSPVFREFYSERNVI
                     LEERRQTLESRPGGLVNEAFNAMTWMAHPYHWEVIGWPSDIAQVTREQANEFFATYYA
                     PNNLTAILVGDLDVEKTFALAERYFGRIPANPKGVPPVVTLEPAQPAEQRMVAEVETT
                     PSAEVDYKAVPAVHADAAALQVLGVVLGGPPMMGRPGMGGPMRPPSGRLHKALVLDQK
                     AATRASASFRGQKYGGLFYVSATPAPGKRPDEVEPLLYAELDKVLKDGVTDDELARAK
                     NAIRVAWYSRLESNAGIRESLAQAESAGTWRDLLGSQKKVEAVTREDVQRVAKKYLVK
                     EGRNVLLTTRKGGGEGMPRRRPGGMPPGGMPGGAPALAPAPAPAPASAPTPEVKG"
     gene            complement(4973..7735)
                     /locus_tag="P5164_07900"
     CDS             complement(4973..7735)
                     /locus_tag="P5164_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43853.1"
                     /translation="MNELRIAPALLLAALVAPAGPAPAAPAKPPKATPAAAAPALPAT
                     PPAAQLAALKARPIGPAVMGGRVVSIAVDPEDPATFYLGHATGGLWKTTNGGTTFSPL
                     MKDAKVFSIGAVAVAPSDPRVLWVGTGEATDRNSAGFGSGVFRSTDGGATWTHAGLAS
                     SRAIARIRVHPGKPEVAFVAASGDLWTKGGERGLFRTTDGGKSWTKVLSAAAPDDADT
                     GCVDVVLSPADPDLVYAALYARRRTPWSFTYGPSLTGGRDAGGIFRSSDGGATWTKLT
                     NGLPEQTGRIGLAVTAADPKVVVAVVQSDAGGTSGIDEIESRAGGVFRSEDGGESFAR
                     VSRFNPRPFYFSQVRLDPANARRVYVLGYALSVSDDGGRTWREDLFGKVHPDVHELVV
                     TGSPLAPRPATPKEGEPPEPAPRPVSPRLLLGTDGGAYLSADAGAAWAHVDRIPSGQF
                     YRISLDDSSPYRICGGLQDNVNWVGPSRTFTKDGIVNADWIQVGGGDGFWCVFDPFDR
                     DVVYAESQEGYVHRMHLGSGQLKRLRPEPTEGQPAFRFHWNSPLVGSRHEKGTMFLAG
                     NRVFRVTEKGERFTPISPDLSTQELAKMRTVGSGAENFGVVYALSESPQRAGLLLAGT
                     DDGKVWVTEDGGAKWTDLTGFLPAEAKGQWVARLEAGRHDEKVAYLAVSAYRAGNHAP
                     LFYRTADLGRTWTSVSGDLPKDEPADVIREDPRNPDLLYAGTEHGFYASLDRGRTWTK
                     LGDLPTVPVDDVAIHERDRDLVVATHGRSLFVLDDVTALQELTPEVRGKALHLFPPRP
                     AEARHLLPGWADWNGVAHFRGENPKEGALLTFWLKGPSAEPVKVAVTTADGRPVANLT
                     APALAGLGRVSWDLKPTRDLRTEYGGLPADRHVAPGDYRVTVTCGEAKSTQTLTVTAA
                     AGVETR"
     gene            complement(7732..8550)
                     /gene="proC"
                     /locus_tag="P5164_07905"
     CDS             complement(7732..8550)
                     /gene="proC"
                     /locus_tag="P5164_07905"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRY43854.1"
                     /translation="MLSGKRIAIIGSGTMGRALAGGLLRSGKVEAGQLACTARTRPTA
                     EKLAAELGLACTTDNAEAARGADVLVLCLKPRDVLRACEALGAKGAVAHAPLVVSIAA
                     GVSTAALQEALGEGIPVVRAMPNTPCFIGKGMTVVSKGVHATEAHSAVALEIFSTMGR
                     TMELEEKHMDTVTALSASGPAFLYVVLESLADGGVMRGLPRAAAIELAAQAALGAAEM
                     VLQTGRHPAALKDDVTTPAGCTIAGILAMEDGRIRSVLARGIETAARAAGELGK"
     gene            8713..>10097
                     /locus_tag="P5164_07910"
     CDS             8713..>10097
                     /locus_tag="P5164_07910"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRY43855.1"
                     /translation="MRLFAPGTALGARFEIGAVRGVGGSAVVYSAFDRELKQTVALKV
                     LRADRTSPAALLRMKREVAIARQAASPRLVRVYDIDASGESVFLTMEDVPGGSLKDRL
                     EEGPIPVEEALRVAGEILEGLAVLHGLGIVHRDVKPGNVLLAIDGSVKLADFGLARRL
                     EAAETRATSMDAVVGTIDYLSPEQALGRELDGRSDLYSFGVTLFEMLTGAVPFRRDSA
                     VGTALAHVRDPAPRLRSARPEMPTWLEAFVGRLLAKEPEDRYATAEAALADLRGRRST
                     KLPVKSRRRRRAVLGALAAIGVVLVLAAAALAGARLRRPEAVRFGPHANGRGVVALDG
                     SGRALWERPDLPNGSAARTFRRPGGKAGIAAVLAVASGEREVGATIALLEPASGALRK
                     EIRVAVPGPLGWWDFPERWVFGALEPIDTDGLDGEELVLTLIHPELYPSAAFLVDPET
                     GTSRFLLAESS"
BASE COUNT         1277 a         3542 c         3809 g         1469 t
ORIGIN      
        1 actcgctctg ggtctcgacg tactgctcgt agtcgtccac gtcgaacatc gccatggcgg
       61 tgtcctgcac gcgccagatg acgacggcgg cgatctcgat cgggttgccc cccttgtcgt
      121 tcaccttcag ccgctggccg ttcagcgtcc gggcgcgcag ggagatccgg ttccgcttcg
      181 aggagcgccg gccggcggcc tgcgcggcct gggcggcgag ggccgccggc ttcgcgccgt
      241 gcccctcggc ggaggcggcg gcggccgccg cggcggccgc cgcgccggac ccgttcgagt
      301 agaacgggtt cccccagtgg aagccggcct cccggacggt ccccttgtac gcgccgaaga
      361 ggacgaggac ccgggcctcg ttcggctgga gcgtgaagaa gccggtgacg aagacgatcc
      421 aggcggggag tcccagcagg gagacgacga aggggaggac ataggggtcc cccttcgcct
      481 gcacgcccgc ggcgatcgac cagacgaaga ggccgatgtc cgcggcgagg aggagaaggg
      541 cgaagacgag catcggccac ccgttgtgga ccgtcgccgg aacttcccgg ttgacgctca
      601 gggtgttcgg tttcatggcg cgacctcctg tcggctgcta ccgaagtgat atcaccgtga
      661 aatacgcggc gtcaagaaga aggtttcggg tcgccggcgg gcgcctcacc cgttcggggg
      721 atgccgcagt gcaggaggga gcggcatagt cggaccgact ttacggagac gggacgtgcg
      781 gcgggccgga gcggcgcgac gggaggtcga tacggcggat cgctctccag gcggcgagcg
      841 gaggagacgg gaaaaggggc ccgcggctgg gcgtgcggcc gcgagcgggg cggccctccc
      901 gaccacaccg cgctgcgact cgtgcgacgt cgcgaagacc tacgcggccg agaaggcgcg
      961 gctcgccgtc tggctcgcgc acgagctccg gacgccgctg aattgcgtcc tcggctacac
     1021 cgagctcctc tgcgaccagc tcgaggcgcc ggggcatgca acgctcctcg ccgacgccct
     1081 gcgcgtgagg gacctcgcgc tccatcagct cacgctcctc gacgaggtgc tcgacctcgc
     1141 ccggctcgag cacgggagcc tggccctgag ggtcgagacg atcgacgcga cacgggtcct
     1201 cgaggggatc gcggccgcgt cgctcccgat cgtgaggcgg ggagggaacc gggtcctcct
     1261 gacgaccgag gggccgacct gggtcctcgc cgacgagacg cgtctccggc aggtcctcct
     1321 gaacctcgtc gggaacgccg cgaagttcac ccgcgacggc ctcgtcacgc tcggagcgac
     1381 ggaggcgaac ggctccgtcc tcctccacgt cagcgacacc ggtccgggga tggccccggg
     1441 ggtggtggag cgtctgttcc gcccgttcca gcaggcgagc gcggccgtcg ccgccgcgca
     1501 cggaggctcg ggcctgggtc tggcgatctc gaaggagctg tgcgagagga tgggcgggtc
     1561 gatctcgctg cggacggccg agggagaggg gacgaccttc acggtcaccc tccgcgcggc
     1621 cccgaagcct cgcccggccg ccggcagcgg cggccgggcg aagacagagg tcagggaatc
     1681 ggcttcagcg tgagcgggtc gcggagcggg aggcgcgcga gcgggagctt cgcggcgtcc
     1741 ttcagcgcgc ccgggtggtc ggcgtcgccc gcctcgatct cggaggcctt gccgaccgca
     1801 aggacgacca tcttctcgaa gtcgaggagc ttcctcgccg ccttctggac gtcggcggcg
     1861 gtcacggcct tcaccttctc gcggtagtcg agccaccagt cttcgggcca gccgttcagg
     1921 ttctcgtcgg cgaagcggtt cgcgacggcc tggcggcttc cccactgcgc ggggaacgct
     1981 tcgacgaggc cgtccttgat cgaggcgagc tcggcttccg tgagcggctc gtccttgagc
     2041 cgctgcatct cggcgagcgc gaggcggagg gcgtaggcca cggtggcatt cttcgtctga
     2101 aggcccccgg tcaggtcgcc gcgccagtac ggcccctcgc cgagctgcgt cctcacgccg
     2161 taggtgagcc cctcgtcgga gcggatcttc ttcatgaggc gcgccgcgaa gccgccgccg
     2221 ccgaggacgt ggttcatgac catcgccggg taccagtcgg ggtcggagcg gcgcatcccg
     2281 gggaaggccc actggagcat cgcctgcggg gcgtccttgt cggtgacgta gagaccgggc
     2341 ttgcgagcga actcgggcag cgggaccttc gggctcggct tcgccgccgg gccagccttg
     2401 atcgcgccga cggtgcggtt caggaggtcg gtcatcgccc tcctctcgag cttcccggag
     2461 acctcgacga cgaggttggc ggggtggagg agcgaggcgt ggagggcgag gaggtcctcg
     2521 cgcgtgatcg cgtcgaggct cgcggccgtc gtccgctccg tcgcgaagaa cccctcgccg
     2581 aagagcaggg ccggcatctg gagctgtgcg atcgaggtga cggcgtcgtt ccgccgctcg
     2641 agcccctggc gggcgttctt cttcgcgagg tcgagccggt cctgcgcgaa ggcgggctgc
     2701 gtgagggcct gcatcaggag agcgagcccc tcggcgaggt ccttctcctg gaccctgatg
     2761 tggatgcctc cgctcgtcgg cccgcagctc gacgagagcg tcgcggcgag cgcctcgagg
     2821 cggtcctcga gcttcgcggc gtcggtcttc tgcgtcccgc cgacggcgag ctgggagccg
     2881 aagaggctcg cgagcccctc cttgccggcg gggtcgaggt aggcgccgcc gcgccaccag
     2941 acgctgaccc ggacgagcgg gacgccggac gggtcggcga cgaggtacgc cgggaccttg
     3001 ttcttcagga cgaccttcgc gtccttcgcc ttcgggacct cccacgagat cgcggggtag
     3061 gtcagcttct cggggcggtc ggggatggcc tgcccgaggg cggtcgtcgc cgcgaaggcg
     3121 gcgaggagcg cggcgttctt cgcgaggcgg ttcatccctt cacctccggg gtcggggcgg
     3181 aagcgggcgc gggggccggg gcgggagcga gagccggggc tcctccgggc atccctccgg
     3241 gcggcatgcc gccgggacgc cggcgcggca tcccctcgcc gccccccttg cgggtcgtca
     3301 ggaggacgtt gcggccctcc ttcacgaggt acttcttcgc cacgcgctgc acgtcctcgc
     3361 gcgtgacggc ctcgaccttc ttctgcgacc cgaggaggtc gcgccacgtg cccgcgctct
     3421 cggcctgcgc gagcgactcg cggatgccgg cgttgctctc gaggcgggag taccaggcga
     3481 cgcggatcgc gttcttggcg cgcgcgagct cgtcgtcggt gacgccgtcc ttcaggacct
     3541 tgtcgagctc ggcgtaaagg agcggctcga cctcgtcggg gcgcttgccg ggagcgggcg
     3601 tcgccgagac gtagaagagg ccgccgtact tctgcccgcg gaacgacgcc gaggcgcgcg
     3661 tcgcggcctt ctggtcgagg acgagcgcct tgtggagccg gcccgacggg ggccgcatcg
     3721 gcccgcccat cccgggacgg cccatcatcg gcggcccgcc gaggacgacg ccgaggacct
     3781 ggagcgccgc cgcgtcggcg tggacggccg ggacggcctt gtagtcgacc tcggcggagg
     3841 gggtcgtctc gacctcggcg accatccgct gctcggcggg ctgcgccggc tcgagcgtga
     3901 cgacgggcgg gacccccttc gggttcgcgg ggatccggcc gaagtacctc tccgcgaggg
     3961 cgaacgtctt ctcgacgtcg aggtcgccga cgaggatcgc ggtgaggttg ttcggcgcgt
     4021 agtaggtcgc gaagaactcg ttcgcctgct cgcgcgtcac ctgggcgatg tcgctcggcc
     4081 agccgatgac ctcccagtgg tacgggtggg ccatccaggt catcgcgttg aacgcctcgt
     4141 tcacgaggcc gccggggcgc gactcgagcg tctgacgcct ctcctcgagg atgacgttcc
     4201 gctcgctgta gaactcgcgg aagacggggc tgccgagccg gtcggactcg agccacatcc
     4261 agagctcgag cttgttcgac gggaggccga cgatgtagaa ggtccggtcc tggctcgtcg
     4321 aggcgttgag ccccgtcgcg ccgttctcct ggtagacctt gtccatctcg tccttgacga
     4381 tgagcgcccg ctgctccttc acgagcgcgt cgaacttcga gagcagctcc tgaaggcgcg
     4441 gcgtgcgagc ggcgggcgac atcatgtcgg cgatctcgcc gcggcgctgc ttctcgcgaa
     4501 ggagcgtcat ctcctcgcgg atctgcgcct ggacctcgtc ctgggcccgg ttcaactcgg
     4561 cgtcgcgcgc ggcgtcgcgc gttccgatcg tcgtcgtccc cttgaacatc atgtgctcga
     4621 agagatgcgc gaggccggtg atgccggggc gctcgttcgc gctcccgacg cgggcgagcc
     4681 agccgcacga gatcgtcggc tggtcgtgcc gcgggaccat caggagcttc atcccgttcg
     4741 gcaaggtccg ctcgacgacg ttcagctcct gggcggagag gggggccgcg agaatggccg
     4801 ccgcgagcag ggcggcgggg gcgcggcctc ggaagacggt catgactcct cctcgtgggg
     4861 gcggtcggaa acgcgatggc ccgcgcggcg cgcgggccat cgggcattct aggtccgggg
     4921 aggcctccgc actctctgcc ccgccgcggg ggccgccccc gccgccgcgc ccctaccggg
     4981 tctcgacgcc ggccgccgcg gtcactgtca gcgtctgcgt gctcttcgcc tcgccgcagg
     5041 tcacggtcac gcggtagtcg cccggggcga cgtgccggtc ggccgggagg ccgccgtact
     5101 cggtccgcag gtccctcgtc ggcttcaggt cccacgagac gcgcccgagg ccggcgaggg
     5161 cgggcgccgt caggttcgcg acgggccggc cgtcggccgt cgtgaccgcg accttcaccg
     5221 gctcggccga aggccccttc agccagaacg tcaggagtgc cccctcctta gggttctcgc
     5281 cgcggaagtg cgcgacgccg ttccagtcgg cccagcccgg caggaggtgc cgcgcctcgg
     5341 ccgggcgcgg cgggaagagg tggagcgcct tcccgcgcac ctcgggcgtc agctcctgga
     5401 gggccgtcac gtcgtcgagg acgaagaggc tccgtccgtg tgtcgcgacg acgaggtcgc
     5461 ggtcccgctc gtggatcgcc acgtcgtcga ccgggaccgt cgggaggtct ccgagcttcg
     5521 tccaggtgcg gccgcggtcg agggaggcgt agaagccgtg ctccgtcccg gcgtagagga
     5581 ggtccgggtt cctcgggtcc tcgcggatca cgtcggccgg ctcgtccttc gggaggtccc
     5641 ccgagacgga ggtccaggtc cggccgaggt cggcggtccg gtagaagagc ggggcgtggt
     5701 tcccggcgcg gtaggccgag acggcgaggt acgcgacctt ctcgtcgtgc cgccccgcct
     5761 cgaggcgcgc gacccactgc cccttcgcct cggcggggag gaagccggtg aggtcggtcc
     5821 acttcgcccc tccgtcctcg gtcacccaca ccttcccgtc gtcggtcccg gccaagagga
     5881 ggccggcccg ctgcggtgac tccgagaggg cgtagacgac gccgaagttc tcggcgccgc
     5941 tcccgacggt acgcatcttc gcgagctcct gcgtcgagag gtcggggctg atcggcgtga
     6001 accgctcccc cttctccgtc acgcggaaga cgcggttgcc ggcgaggaac atcgtcccct
     6061 tctcgtgccg gctcccgacg agcggcgagt tccagtggaa gcggaaggcg ggctgaccct
     6121 ccgtcggctc gggacgcagc ctcttcagct gcccgctccc gaggtgcatc cggtggacgt
     6181 acccttcctg cgactcggcg tagacgacgt cgcggtcgaa cggatcgaag acgcaccaga
     6241 agccgtcgcc gccgccgacc tggatccagt cggcgttgac gatcccgtcc ttcgtgaagg
     6301 tgcggctcgg gccgacccag ttcacgttgt cctgcagacc gccgcagatc cggtacgggc
     6361 tcgagtcgtc gagcgagatc cggtagaact gccccgacgg gatccggtcg acgtgcgccc
     6421 aggccgcccc ggcgtcggcg ctcaggtagg cgccgccgtc ggtcccgagg aggaggcgcg
     6481 gagagaccgg cctgggcgcc ggctcgggcg gctcgccctc cttcggcgtc gcggggcggg
     6541 gcgcgagagg gctccccgtc acgacgagct cgtggacgtc ggggtggacc ttcccgaaga
     6601 ggtcctcgcg ccacgttcgg ccgccgtcgt cggagacgga gagcgcgtag ccgaggacgt
     6661 agacgcgacg ggcgttcgcc gggtcgagcc gcacctgcga gaaatagaac ggccgcgggt
     6721 tgaaccgcga aacgcgcgcg aagctctcgc cgccgtcctc cgagcggaag acgccgccgg
     6781 cgcggctctc gatctcgtcg atcccgctcg tcccgccggc gtcgctctgg acgaccgcga
     6841 cgacgacctt cgggtccgcg gcggtgacgg cgaggccgat ccgccccgtc tgctcgggga
     6901 ggccgttcgt cagcttcgtc caggtcgccc cgccgtcgga cgaccggaag atcccgcctg
     6961 cgtcgcgtcc cccggtgagc gaggggccgt acgtgaacga ccagggcgtc cgccgccggg
     7021 cgtagagcgc ggcgtagacg aggtcggggt cggcggggga gaggacgacg tcgacgcagc
     7081 cggtgtccgc gtcgtcgggc gcggcggccg agaggacctt cgtccagctc ttcccgccgt
     7141 ccgtcgtccg gaagaggccg cgctcgcccc ccttcgtcca gaggtcgccg gacgcagcga
     7201 cgaaggcgac ctcgggcttg ccggggtgga cgcggatccg cgcgatcgcg cggctcgacg
     7261 cgaggccggc gtgcgtccac gtggcgcctc cgtccgtcga gcggaagacg ccgcttccga
     7321 agcccgcgct gttgcggtct gtcgcttcgc ccgtccccac ccagaggacg cgcgggtcgg
     7381 acggggcgac cgccacggcg ccgatcgaga agaccttcgc gtccttcatg aggggcgaga
     7441 aggtcgtccc gccgttcgtc gtcttccaga gcccgcccgt cgcgtggccg aggtagaagg
     7501 tggccgggtc ctccgggtcg acggcgatgg agacgacgcg gccccccatg accgccgggc
     7561 cgatcgggcg cgccttcagc gcggcgagct gcgccgccgg gggcgtcgcg gggagcgccg
     7621 gggccgcggc ggcgggagtc gccttcgggg gcttcgcggg cgcggcgggg gcggggccgg
     7681 cgggggcgac gagagcggcg aggagaaggg ccggggcgat gcggagctcg ttcacttccc
     7741 gagctccccg gcggcgcgcg cggcggtctc gatcccgcgc gcgaggacgg agcggatccg
     7801 gccgtcctcc atcgccagga tgccggcgat cgtgcagccg gcgggggtcg tcacgtcgtc
     7861 cttcagcgcc gccgggtggc gccccgtctg gaggaccatc tccgccgcgc cgagggccgc
     7921 ctgcgcggcg agctcgatgg cggcagcgcg gggaaggccg cgcatgacgc cgccgtccgc
     7981 gagcgactcg aggacgacgt agaggaacgc cgggccgctt gccgagaggg cggtcacggt
     8041 gtccatgtgc ttctcctcga gctccatcgt ccgccccatc gtcgagaaga tctcgagggc
     8101 gaccgcggag tgggcttccg tcgcgtggac ccccttcgag acgaccgtca tccccttccc
     8161 gatgaagcag ggcgtgttcg gcatcgcccg gacgaccggg atcccctcgc cgagcgcctc
     8221 ctgcagagcg gccgtcgaga cgcccgccgc gatcgagacg acgagcgggg cgtgcgcgac
     8281 cgcccccttt gcgccgaggg cttcgcacgc cctgaggacg tccctcggct tcaggcagag
     8341 gacgaggacg tcggcgccgc gggccgcctc ggcgttgtcg gtcgtgcagg cgaggccgag
     8401 ctccgcggcg agcttctcgg ccgtcgggcg ggtccgggcc gtgcaggcga gctggcctgc
     8461 ctcgaccttg cccgagcgga ggagccctcc tgccagcgcg cgtcccatgg tccccgagcc
     8521 gatgatggcg atccgctttc cgctgagcat cgatccgtct cctgtcccgt cgaggtgcgg
     8581 gcggatcgta cgcgacgccg acgagcggcc gggtcgcgcc gtagaatggc tcgctgcgga
     8641 ggcctcctgg acgacggcac cgggacgatc gaggccggat tgcgctcgga ccgcggtgcc
     8701 gaggggcacg aggtgcgcct tttcgccccg gggaccgcgc tcggcgcccg gttcgagatc
     8761 ggcgcggtgc gcggcgtcgg cggatcggcc gtcgtctact cggcattcga ccgcgagctg
     8821 aagcagaccg tcgcgctgaa ggtcctgcgc gccgaccgga cgagccccgc ggcgcttctc
     8881 cggatgaaga gggaggtcgc gatcgcgagg caggccgcga gcccgcgcct cgtccgcgtc
     8941 tacgacatcg acgcctcggg ggagtcggtc ttcctgacga tggaggacgt ccccggcggc
     9001 tcgctgaagg accggctcga ggaaggcccg atcccggtcg aggaggcgct tcgcgtcgcc
     9061 ggcgagatcc tcgaggggct cgccgtcctc cacggtctcg gcatcgtcca ccgcgacgtg
     9121 aagccgggga acgtcctcct cgccatagac ggctccgtga agctcgccga cttcggcctg
     9181 gcgcgccggc tcgaggccgc cgagacgcgt gcgacgtcga tggacgccgt cgttggcacc
     9241 atcgattacc tctcgcccga gcaggctctc ggccgcgagc tggacggccg gagcgacctc
     9301 tattccttcg gcgtcacgct cttcgagatg ctgaccgggg ccgtcccctt ccgccgcgac
     9361 tcggccgtcg gcaccgcgct cgcgcacgtg agggatccgg cgccgcgcct ccgctccgcc
     9421 cggcccgaga tgccgacgtg gctggaggcc ttcgtcgggc gactcctcgc caaggagccg
     9481 gaggatcggt acgccacggc cgaggcggcg ctcgccgacc tccgggggcg aaggtcgacc
     9541 aagctcccgg tgaagagccg gagacgacgc cgggccgtcc tgggcgcgct cgccgcgatc
     9601 ggcgtcgtcc tcgtcctggc ggccgcggcc ctggcgggcg cgcggcttcg gagacctgag
     9661 gcggtccgct tcggcccgca cgcgaacggc cgcggcgtcg tggcgctcga cggctcggga
     9721 cgtgcgctct gggaacggcc cgacctgccg aacgggtcgg ccgcccggac gtttcgaagg
     9781 cccggtggga aggcggggat cgccgcggtt ctcgcggtcg cgtcgggcga gcgcgaggtc
     9841 ggcgcgacga tcgcgctcct cgagcccgcg tcgggcgccc ttcggaagga gatccgagtc
     9901 gcggttcctg gcccgctcgg ttggtgggac ttccccgagc gctgggtgtt cggggcgctc
     9961 gagccgatcg acacggacgg gctcgacggg gaggagctcg tcctcaccct gatccatccc
    10021 gagctctacc cgtccgcggc gttcctcgtc gacccggaga cggggacctc ccggttcctt
    10081 ctggcggaga gcagcca
//
LOCUS       DAOTEZ010000118        10068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4724_length_10068_cov_6.990113, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000118 DAOTEZ010000000
VERSION     DAOTEZ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10068
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4724_length_10068_cov_6.990113"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /gene="tssC"
                     /locus_tag="P5164_07915"
     CDS             complement(<1..914)
                     /gene="tssC"
                     /locus_tag="P5164_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HRY43856.1"
                     /translation="MSNATERGKADAIVREKEGEVNLLSKILEEGRLVQHENQAQQAR
                     DMIAEFVQQAMQGQMVLSRNLEASVQARIAEIDRLLSAQLNEIMHSEPYQKLEGSWRG
                     LHHLVYESETSVMLKIRVLNVSKKDLLKDLERAAEFDQSAVFKKVYEEEYGTFGGQPF
                     GALIGDFYFSNHPQDMLLLEKMAQVAAAANAPFIAASAPQLFGWESFQEMTEVRDLAK
                     IFDRTEYAKWRSFRDSEDSRYVGLTMPRTLMRLPYGKETIPTETFNFEEDVDGKDHNK
                     YLWGNAAYSFGTRLTEAFAMYGWCVAIRG"
     gene            complement(907..1443)
                     /gene="tssB"
                     /locus_tag="P5164_07920"
     CDS             complement(907..1443)
                     /gene="tssB"
                     /locus_tag="P5164_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HRY43857.1"
                     /translation="MVKKESIQQKLKRVRPPRVQITYDVEIGGAIELKELPFVVGVLG
                     DFSGQPEQPLPRMKDRKITEIDRDNFDQVLAGMAPRVATSVENKLAGDGSKMAVDLKF
                     QSLEDFEPDKVVQQVEPLRKLVEARQRLSDLMSKADGNEKLEKMLNDVLQNSGSQQQL
                     ASALGLTPEGPKKGDGNE"
     gene            complement(1496..2617)
                     /gene="tssA"
                     /locus_tag="P5164_07925"
     CDS             complement(1496..2617)
                     /gene="tssA"
                     /locus_tag="P5164_07925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HRY43858.1"
                     /translation="MSSEAPLLDFDRLLAPLSDERPSGESLQYAGLYDEIREARRADD
                     QLEQGDWKRELKVADWDEAVALATDALLTKTKDLQVAAWLAEGLTMLHGVAGARDGIR
                     LMKGLHADFWDTCYPEVDEGDMEGRANVLSFFDRQVALALRKAPLTRAPGMNYSWLEW
                     SESRPFDVPDTAAGPLDGDTQARLADLKRQAAEENKTSGEDFRKAKSSTPKVFYEEAS
                     KALAVAWDEYQALDRVMDEKFGRQTPGLGELKVALEEVRRVVDGILKEKRALEPEQAA
                     PGEAAAEAAGDVPAAGGPFRAAGPAGPVRSRSEALARLGEVAEFFRQTEPHSPVSYLV
                     QRAISWGQMPLETWLADVIKDTNVLGGIKETLGIRPGTE"
     gene            2748..3557
                     /locus_tag="P5164_07930"
     CDS             2748..3557
                     /locus_tag="P5164_07930"
                     /inference="COORDINATES: protein motif:HMM:NF018699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
                     /protein_id="HRY43859.1"
                     /translation="MPTPKELLDSGNLKAAIEALTQEVREAPADPQKRTFLFELLAFA
                     GEWDRAEKQIVAAAGVDAPSQMAAGVYRGLLQAERDRARVFAGKARPHFLRPVPSGVE
                     KTLAALGRISEGDLAGARTLLDEAEEERPALAGRRGEAPFEDLRDFDDRIGGVVEMVF
                     QGKYTWFPLEYARSLEPNAPKKLRDLIWPGARITADDGTSADVFLFALYPGSAEHEDD
                     LVKLGRMTDWKSLSEDLQAGLGLRSILVDGEDVSLFELGPIELEEREPLPS"
     gene            complement(3676..4167)
                     /locus_tag="P5164_07935"
     CDS             complement(3676..4167)
                     /locus_tag="P5164_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HRY43860.1"
                     /translation="MGGLVDYFLKIDGIEGESADSKHKGEIDVLSWSWGASQSGTMQF
                     GGGGGAGKVQMQDFSFTMRINKATPKLFLHCANGAHIKQAVLTARKAGKEQQEYLKLT
                     FSDILVSSYQTGGSGDSDVVPIESISLNFAKIEFEYKEQKADGTLSGPIKTHYDLKSN
                     KGG"
     gene            complement(4276..5301)
                     /gene="tagF"
                     /locus_tag="P5164_07940"
     CDS             complement(4276..5301)
                     /gene="tagF"
                     /locus_tag="P5164_07940"
                     /inference="COORDINATES: protein motif:HMM:NF021393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagF"
                     /protein_id="HRY43861.1"
                     /translation="MAAWSLISKLKEGFAGADGPPRMETCALHLYGKLPIYKDFISSG
                     LTEEGAKEFRDWLGNGFSRRWANDDEYREAEIPLHTFLLPLPGGRRSVTGALWGSHDE
                     GGLRRFPFTLFSVVPQGKAIADPLVALSYLEVFEERATQIRRTYAAGRTLASFYEAYR
                     GAKIEVPVKRPAKIAEGVEKEIRDVTLGDFAESLLGEGAAASWPKFLARLRAACDAPP
                     GEGAGAVRVPVGNLLPATAQLQLWLAWLEAEGAFGRRAPSGVLVCRAGGRSRAVLFFR
                     ELRAEDFLLLHPERSDHEFVEEAVPARGEAAPEGPGDVEAPEAGKRPRGWDRPLAGFL
                     RPEGRET"
     gene            complement(5382..9656)
                     /locus_tag="P5164_07945"
     CDS             complement(5382..9656)
                     /locus_tag="P5164_07945"
                     /inference="COORDINATES: protein motif:HMM:NF025689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein IcmF/TssM N-terminal
                     domain-containing protein"
                     /protein_id="HRY43862.1"
                     /translation="MYELLDLLYRLPKPIKTLGIVVLLSPLCLVVARALGFGHLWWAV
                     IGGLILIALLVAAFDVFVKKGDKARGGAFEKDLARDAQGGAGASKQEVREAVKELSQK
                     WTESLAQLKATGLQVYSLPWYLLIGEPQSGKSTTLKNSGLEFPVGGDGLSGSGGTRNC
                     DWWFANEAVILDTAGRFTFQEEAAPDATEWSTFLKMLKKYRKECPINGVLVVVPATSL
                     LEDTPEEQDKKAANIRTKLLHLQRVLEIRFPIFIMVTKADRILGFSEFFSKLDPVDQR
                     QLVGWSNPNGPDKPYALASFDEAWEEILTRVHKLRMKFTANEEIVQNVDRIFVFPEEL
                     RALGSSLKRYFQGIFQETRYDDPFVFRGFYLSSGVQQGKPIARATRELLGSSAEGIVE
                     NLEQIFKRSRAFFIKDFYEKKVFPEQGLIARTKAALEREKTTRWVIRAVTVFLPIFCL
                     LGMILPYVAMRKFVKPMRETALKAQACVEKKEACSIGEAYGIAESIYKHQAAIRQRPF
                     LFALFFQRASSGELFRLLGKINQRLTYERVVNPLMAEGEARMARLDWGGPYDYRVFFT
                     ALDGHMKWWAVKKGALGAEQKPLVAASELKLIPLLAFLQNTKGAAGTAASKEIDEWVA
                     SIGSSGTPDEILAALPADPPKDPGLIRVPDPAGALKKFEEYWSVANLARWDFRLNQLL
                     AQYVQRYSELLKIEDPSSRGYLDRVAATGKEFRKVYDDTTKHLGTARPGTKEFPGAGP
                     DDWKKNFDADYSQLMRFTTVVPGVVNEQARSRIKGQMDSDWKNLEVQVLAYAYLVVPD
                     AQDPAKKGWSPSAAAISPMVADATAFADLAAFEAAENPAAVVEEAARKSTPADQKATL
                     EAFTKKQVDAKAKAVANLTAIPAIPAEQKTLWQDTALAAFLPRTADLALVYRVLPYTQ
                     QFLDRSVGPACLENVCFTRNHAQQMVPLGVSYISFAEASSISTRPDVDALAQGHAEAL
                     LDYLRRYVDRFAGRGGGGGGGGFVVPGSALRASSWREFQAAITRWSPESQGGDAPVTD
                     MSGALAFDDVQTFAASNRRLASILPYFKGKAAPRRSTGPATAPPELVAAANLFKNNVV
                     PLPEQELKAWRQLSLGQDGVSLRGFHAFSRNPAVRRSSYGRQLIAVENRGANLIRDAI
                     QPSFQQAAEPVVRKIREQCLGQFPFVRESQLRQERVAYAAGSSLKPGGSGNTATYRLD
                     LATIGQNHFSGILTDVGALADEYALNPILWGWEPQFDFVGDAGRGLLAVGRGWQLFLF
                     GGGRSTSVIKEHKVEIRPLQFSPGPGKRFMGDRINSLFLFDRSVVVRPSTDFKTGRQP
                     SPYVWRLGPADAPMSITGRDEQSGSGWTGSLEAYGGPLRFFYFVRVASEERDRRDDSD
                     DSERAREKEKIWTIRVVIPNALQRTEPLEGLFELAFEEPMPPILPN"
     gene            complement(9664..>10068)
                     /locus_tag="P5164_07950"
     CDS             complement(9664..>10068)
                     /locus_tag="P5164_07950"
                     /inference="COORDINATES: protein motif:HMM:NF021377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HRY43863.1"
                     /translation="LEEKYFRSNIGGDQYFAIAKELRPEDVELGSIVYAGLALGFRGK
                     YRERPEKLAEVRKNTYRILSEYLASQSDKITPEAYTVMASSGKRLNPVVTLGRVAIVS
                     FSVLIGFWILTFVAWATLVGQVKSVAEAMGVI"
BASE COUNT         1449 a         3520 c         3446 g         1653 t
ORIGIN      
        1 ccccggatcg cgacgcacca gccgtacatc gcgaacgcct ccgtgaggcg ggtcccgaag
       61 gagtaggccg cgttccccca gaggtacttg ttgtggtcct tcccgtcgac gtcctcctcg
      121 aagttgaacg tctccgtggg gatcgtctcc ttgccgtagg ggaggcgcat cagcgtccgc
      181 ggcatcgtga ggccgacgta gcgggagtcc tccgagtccc ggaacgagcg ccacttcgcg
      241 tactccgtcc ggtcgaagat cttcgcgagg tcgcgaacct cggtcatctc ctggaagctc
      301 tcccagccga agagctgggg ggccgaggcc gcgatgaacg gggcgttcgc cgccgccgcg
      361 acctgcgcca tcttctcgag gaggagcatg tcctgcgggt ggttcgagaa gtagaagtcg
      421 ccgatgaggg cgccgaacgg ctggccgccg aacgtgccgt actcctcctc gtagaccttc
      481 ttgaagacgg ccgactggtc gaactccgcc gcccgctcga ggtccttcag gaggtccttc
      541 ttcgagacgt tcaggacgcg gatcttcagc atcaccgacg tctcgctctc gtagacgagg
      601 tgatggaggc cgcgccagga gccctcgagc ttctggtacg gctcggagtg catgatctcg
      661 ttcagctgcg ccgacaggag ccggtcgatc tcggcgatgc gcgcctggac cgacgcctcc
      721 aggttgcgcg acaggaccat ctggccctgc atcgcctgct ggacgaactc ggcgatcatg
      781 tcgcgcgcct gctgcgcctg gttctcgtgc tggacgaggc gcccctcctc caggatcttc
      841 gagaggaggt tgacctcgcc ttccttctcg cggacgatcg cgtcggcctt gccgcgttcg
      901 gtcgcgttac tcatttccgt cccccttctt cggcccctcg ggcgtgaggc ccagcgcgct
      961 cgcgagctgc tgctggctcc cggagttctg gaggacgtcg ttcagcatct tctcgagctt
     1021 ctcgttcccg tcggccttcg acatgaggtc cgagaggcgc tgccgcgcct cgacgagctt
     1081 ccggagcggc tccacctgct ggacgacctt gtccggctcg aagtcttcga ggctctggaa
     1141 cttcaggtcg accgccatct tcgacccgtc gccggcgagc ttgttctcga cggacgtcgc
     1201 cacgcgcggg gccatccccg cgaggacctg gtcgaagttg tcgcggtcga tctcggtgat
     1261 cttccggtcc ttcattcgcg ggagcggctg ctcgggctgg cccgagaagt cgccgagcac
     1321 gccgacgacg aacgggagct ccttgagctc gatcgcgccc ccgatctcca cgtcgtacgt
     1381 gatctgcacg cgcggcggcc ggacccgctt gagtttctgc tggatgcttt ccttcttcac
     1441 catgagcgtt cctccctaga agtccgtcgg cttctctgtt cgcctgctgt cgcgcctatt
     1501 cggtcccggg ccggatcccg agagtctcct tgatcccgcc caggacgttc gtgtccttga
     1561 tcacatccgc gagccacgtc tcgagcggca tctgccccca cgagatcgcg cgctggacga
     1621 ggtaggagac ggggctgtgc ggctcggtct ggcggaagaa ctcggccacc tcgccgagcc
     1681 gggcgagcgc ctcgctccgg ctgcggaccg gtcccgcggg gcctgccgcg cggaaggggc
     1741 ctccggccgc ggggacgtct cccgccgcct cggccgccgc ctctcccggc gccgcctgct
     1801 ccggctcgag cgcccgcttc tccttgagga tcccgtcgac gacgcggcgg acctcctcga
     1861 gagcgacctt cagctcgccg aggcccggcg tctggcgtcc gaacttctcg tccatgacgc
     1921 ggtcgagcgc ctggtactcg tcccacgcga ccgcgagcgc cttcgacgcc tcctcgtaga
     1981 acaccttcgg cgtcgacgac ttcgccttcc ggaagtcctc gccgctcgtc ttgttctcct
     2041 cggccgcctg ccgcttcagg tcggcgaggc gcgcctgcgt gtcgccgtcg agaggccccg
     2101 cggccgtgtc cgggacgtcg aaaggccggg actcgctcca ctcgagccag gagtagttca
     2161 tgccgggcgc ccgcgtcagc ggcgccttgc ggagcgcgag ggcgacctgc cggtcgaaga
     2221 acgagaggac gttcgcccgc ccttccatgt cgccctcgtc gacctccggg tagcaggtgt
     2281 cccagaagtc ggcgtggagc cccttcatca gccggatccc gtcgcgcgcg ccggcgactc
     2341 cgtggagcat cgtgagccct tcggcgagcc acgcggcgac ctgcaggtcc ttcgtcttcg
     2401 tcaggagcgc gtccgtcgcg agcgcgacgg cctcgtccca gtcggcgacc ttcagctcgc
     2461 gcttccagtc cccctgctcg agctggtcgt cggcgcggcg cgcttcccgg atctcgtcgt
     2521 agagcccggc gtactggagg ctctcccccg acggacgttc gtccgacagc ggggcgagga
     2581 gcctgtcgaa gtcgaggaga ggagcctccg acgacatcta ggtgcctccc aaagaattgc
     2641 gagttggtgg tactgtagct gactcagcag cccctgtcat cccgtcaatc ccgccgccgg
     2701 gacggcctct gcgccggccg gaggagaagg ggcgtaggag gttcgtgatg ccgactccca
     2761 aggagctcct cgactcgggg aacctgaagg ccgcgatcga ggccctgacc caggaggtcc
     2821 gggaagcgcc cgccgacccg cagaagagga cgttcctctt cgagctcctc gccttcgccg
     2881 gggagtggga ccgcgccgag aagcagatcg tcgcggccgc gggcgtcgac gccccgtccc
     2941 agatggccgc cggcgtctac cggggtctcc tccaggccga gcgcgaccgt gcccgggtct
     3001 tcgccgggaa ggcccgtccg cacttcctgc ggcccgtgcc gtccggcgtc gaaaagacgc
     3061 tcgccgcgct cggacggatc tccgagggag acctcgccgg cgcccggact ctcctcgacg
     3121 aggccgagga ggagcgtccg gccctcgccg gccggcgcgg agaggccccg ttcgaggacc
     3181 tccgcgactt cgacgaccgg atcggcggcg tcgtcgagat ggtcttccag gggaagtaca
     3241 cctggttccc gctcgagtac gcccggagcc tcgagccgaa cgcgccgaag aagctccgcg
     3301 acctcatctg gccgggggcg cggatcacgg ccgacgacgg cacgagcgcc gacgtcttcc
     3361 tcttcgctct ctaccccggc tccgccgagc acgaggacga tctcgtgaag ctggggcgga
     3421 tgacggactg gaagagcctc tccgaggacc tccaggcggg cctcggcctc cgctcgatcc
     3481 tcgtcgacgg cgaggacgtg agcctgttcg agctcggccc gatcgagctc gaggagcgcg
     3541 agccgcttcc gagctgagtc ggaagcgccg tgcgccccgg gaaagggccc gcgtgcgacc
     3601 cccggaaact cgaagccggc ctcccgcgaa ggaggccggc ttcgttccga cgaggttctc
     3661 gggaggggaa agggctcagc cccccttgtt cgacttgagg tcgtagtgcg tcttgatcgg
     3721 cccgctgagg gtgccgtccg ccttctgctc cttgtactcg aactcgatct tggcgaagtt
     3781 cagggagatc gattcgatcg ggacgacgtc gctgtcgccg ctcccccccg tctggtagga
     3841 cgagacgagg atgtcggaga aggtcagctt caggtactcc tgctgctcct tgccggcctt
     3901 ccgggcggtg aggacggcct gcttgatgtg cgcaccgttc gcgcagtgca ggaacagctt
     3961 cggcgtcgcc ttgttgatcc gcatcgtgaa gctgaagtcc tgcatctgca ccttgccggc
     4021 gccgccgccg ccgccgaact gcatcgtgcc ggactgcgaa gcgccccacg accacgacag
     4081 gacgtcgatc tcgcccttgt gcttgctgtc ggcgctctca ccttcgattc cgtcgatctt
     4141 cagaaaatag tcaacgagtc cacccatgtt ctgcttcctc ctttcggcag cttccggcct
     4201 ataagatggg ggtcgccctt cgggaattca aggcccgcgc gacgccccgg cacatcggtg
     4261 tgacctcccg caccttcacg tctcccgccc ctcgggacgc aggaatcccg caagcggacg
     4321 gtcccagccg cgcggccgct tcccggcctc gggagcttcc acgtcgcccg ggccttccgg
     4381 cgcggcctcc ccccgggccg ggaccgcttc ctccacgaac tcgtggtcgg accgctcggg
     4441 gtgcagcagg aggaagtcct cggcgcgcag ctcccggaag aagaggacgg cccgggaccg
     4501 gccgcccgcc cggcagacga ggacgcccga gggggccctc cgcccgaacg ccccttccgc
     4561 ctcgagccac gcgagccaga gctgcagctg cgccgtcgcc ggcagaaggt ttccgaccgg
     4621 gacgcggacc gcgcccgccc cttcgccggg aggagcgtcg caggccgcgc gaaggcgcgc
     4681 gaggaacttc ggccaggacg cggccgcgcc ctcgccgagg agcgactcgg cgaagtctcc
     4741 gagcgtgacg tcgcggatct ccttctcgac cccctccgcg atcttcgccg gccgcttcac
     4801 cggcacctcg atcttcgccc cgcgatacgc ctcgtagaac gaggcgagcg tccggcccgc
     4861 cgcgtaggtc cgccggatct gcgtcgcgcg ctcctcgaac acctcgaggt acgacagcgc
     4921 gacgagcggg tcggcgatcg cctttccctg cgggacgacg gagaagagcg tgaacgggaa
     4981 gcgacggagg ccgccctcgt cgtggctccc ccagagcgcg cccgtcacgg accgccgccc
     5041 tccgggcagc ggcaggagga acgtgtggag ggggatctcg gcctcgcggt actcgtcgtc
     5101 gttcgcccag cggcggctga acccgttccc gagccagtcg cggaactcct tcgcgccctc
     5161 ctcggtgaga ccgctcgaga tgaagtcctt gtagatcggg agcttcccgt agaggtgcag
     5221 ggcgcaggtc tccatcctcg ggggcccgtc ggcgcccgcg aaaccttcct tcagcttcga
     5281 gatgagactc cacgctgcca ctcggtcacc tcgctccttc ggaaacggat cgaaaagggg
     5341 acgggcgggg cccggagaat ccgggccccg cccgagagcg gtcagttcgg caggatcggc
     5401 ggcatcggct cctcgaacgc gagctcgaag agcccctcga gcggctccgt acgctgcagg
     5461 gcgttcggga tgacgacccg gatcgtccag atcttctcct tctcccgcgc ccgctcggaa
     5521 tcgtcggagt cgtcgcgccg gtcccgctcc tcgctcgcca cgcggacgaa gtagaagaag
     5581 cggagggggc cgccgtatgc ctcgaggctg ccggtccacc cggagcccga ctgctcgtcg
     5641 cgccccgtga tcgacatcgg ggcgtccgcg ggaccgaggc gccagacgta cgggctcggc
     5701 tgccggcccg tcttgaagtc ggtcgagggg cggacgacga ccgaccggtc gaagaggaat
     5761 agggagttga tccggtcgcc catgaagcgc ttcccggggc ccggcgagaa ctggagcggc
     5821 cggatctcga ccttgtgctc cttgatgacg gacgtcgagc gcccgccgcc gaagaggaag
     5881 agctgccagc cccggccgac ggcgaggagg ccgcggcccg cgtcgccgac gaagtcgaac
     5941 tgcggctccc agccccagag gatcgggttc agggcgtact cgtccgcgag cgcgccgacg
     6001 tcggtcagga tgccggagaa gtggttctgg ccgatcgtcg cgaggtcgag ccggtaggtc
     6061 gcggtgttcc cgctcccccc cggcttcagc gaggagccgg ccgcgtaggc gactcgctcc
     6121 tgccggagct ggctctccct cacgaacggg aactggccga ggcactgctc gcggatcttc
     6181 cgcacgaccg gctcggccgc ctgctggaac gagggctgga tcgcgtcgcg gatcaggttc
     6241 gcgccgcggt tctcgacggc gatcagctgg cgcccgtagg agctccgccg gacggccggg
     6301 ttgcgtgaga acgcgtggaa gccgcggagg gagacgccgt cctgcccgag ggagagctgc
     6361 cgccacgcct tgagctcctg ctcgggaagc gggacgacgt tgttcttgaa caggttggcc
     6421 gccgcgacga gctccggcgg cgccgtcgcc ggcccggtcg accgccgggg cgccgccttc
     6481 cccttgaagt acgggaggat cgaggcaagc cgccggttcg acgccgcgaa agtctggacg
     6541 tcgtcgaacg cgagcgcgcc cgacatgtcg gtcacgggcg cgtcgccccc ctgcgactcc
     6601 gggctccagc gggtgatcgc ggcctggaac tcgcgccagg acgacgcgcg gagcgccgag
     6661 cccggcacga cgaatccgcc gcccccgcct cccccgccgc ggccggcgaa gcggtcgacg
     6721 tagcggcgaa ggtagtcgag cagcgcctcg gcgtggccct gggcgagcgc gtcgacgtcg
     6781 gggcgcgtcg agatcgacga cgcctcggcg aagctgatgt acgagacccc gagcggcacc
     6841 atctgctgcg cgtgattgcg cgtgaagcag acgttctcga gacaggccgg cccgaccgaa
     6901 cggtcgagga attgctgcgt gtacgggagg acgcggtaga cgagggcgag gtcggccgtc
     6961 cgcgggagga acgccgcgag cgccgtgtcc tgccagagcg tcttctgctc cgccgggatc
     7021 gccgggatcg cggtcaggtt cgcgaccgcc ttcgccttcg cgtcgacctg cttcttcgtg
     7081 aacgcctcga gcgtcgcctt ctggtcggcc ggcgtcgact tgcgtgccgc ctcctcgacg
     7141 acggcggcgg ggttctcggc cgcctcgaac gccgcgaggt cggcgaacgc cgtcgcgtcg
     7201 gccaccatgg gcgagatcgc cgcggccgag gggctccagc ccttcttcgc cgggtcctgc
     7261 gcgtcgggaa cgacgaggta ggcgtaggcg aggacctgga cctcgaggtt cttccagtcc
     7321 gagtccatct gccccttgat ccggctgcgg gcctgctcgt tcacgactcc gggcacgacc
     7381 gtcgtgaagc gcatcagctg cgaatagtcg gcgtcgaagt tcttcttcca gtcgtcgggc
     7441 cccgcacccg ggaactcctt cgtcccgggg cgggccgtcc cgaggtgctt cgtcgtgtcg
     7501 tcgtagacct tccggaactc cttcccggtc gccgcgacgc ggtcgaggta gccccgagac
     7561 gacgggtcct cgatcttgag gagctccgag taccgctgga cgtactgcgc gaggagctgg
     7621 ttcaggcgga agtcccagcg cgcgaggttc gcgacgctcc agtactcctc gaacttcttg
     7681 agcgcccccg cgggatcggg gacgcggatc agcccggggt ccttcggcgg gtccgcgggc
     7741 agcgccgcga ggatctcgtc gggcgtgccg ctcgagccga tcgaggcgac ccactcgtcg
     7801 atctccttcg acgcggcggt cccggccgcc cccttcgtgt tctggaggaa cgcgaggagc
     7861 gggatcagct tcagctccga cgcggccacg aggggcttct gctcggctcc gagggccccc
     7921 ttcttcaccg cccaccactt catgtggccg tccagggccg tgaagaagac gcggtagtcg
     7981 tacgggccgc cccagtcgag gcgcgccatc cgcgcctcgc cttccgccat gagcgggttg
     8041 acgacgcgct cgtaggtcag gcgctggttg atcttgccga ggagccggaa gagctcgccc
     8101 gaggaggcgc gctggaagaa gagcgcgaag aggaaggggc gctggcggat cgccgcctgg
     8161 tgcttgtaga tgctctcggc gatcccgtag gcctcgccga tcgagcacgc ctccttcttc
     8221 tcgacgcacg cctgcgcctt cagcgcggtc tcgcgcatcg gcttgacgaa cttccgcatc
     8281 gcgacgtacg gcaggatcat cccgaggagg cagaagatgg gcaggaagac cgtcacggcg
     8341 cggatgaccc agcgggtcgt cttctcgcgc tcgagggccg ccttcgtccg cgcgatgagc
     8401 ccctgctcgg ggaagacctt cttctcgtag aagtccttga tgaagaacgc ccgcgagcgc
     8461 ttgaagatct gctcgaggtt ctcgacgatc ccctccgccg agctcccgag gagctcgcgc
     8521 gtggcgcggg cgatcggctt gccctgctgg acgccggagg agaggtagaa gccgcggaag
     8581 acgaacgggt cgtcgtagcg cgtctcctgg aagatcccct ggaagtagcg cttcagcgag
     8641 ctgccgagcg cgcggagctc ctcggggaag acgaagatcc ggtcgacgtt ctggacgatc
     8701 tcctcgttcg ccgtgaactt catccggagc ttgtggacgc gggtgaggat ctcctcccag
     8761 gcctcgtcga acgaggcgag ggcgtacggc ttgtcggggc cgttcgggtt cgaccagccg
     8821 acgagctggc gctggtcgac cgggtcgagc ttcgagaaga actcggagaa gccgaggatc
     8881 cggtccgcct tcgtcaccat gatgaagatc gggaagcgga tctcgagcac ccgctgcagg
     8941 tggaggagct tcgtccggat gttcgcggcc ttcttgtcct gctcctccgg cgtgtcctcg
     9001 aggagcgagg tcgccgggac gaccacgagg acgccgttga tcgggcattc cttgcggtac
     9061 ttcttgagca tcttcaggaa ggtcgaccac tcggtggcgt cgggggccgc ctcctcctgg
     9121 aacgtgaagc ggccggccgt gtcgaggatc acggcctcgt tcgcgaacca ccagtcgcag
     9181 ttgcgcgtgc cgccggagcc cgacaggccg tccccgccga cggggaactc caggcccgag
     9241 ttcttcagcg tcgtcgactt gccggactgc ggctcgccga tgaggaggta ccacgggagc
     9301 gagtagacct ggagcccggt cgccttgagc tgcgcgaggg actccgtcca cttctgggag
     9361 agctccttga cggcctcgcg gacctcctgc ttggaggcgc cggcgccgcc ctgggcgtcc
     9421 cgcgcgaggt ccttctcgaa ggcgccgccg cgcgccttgt cgcccttctt gacgaagacg
     9481 tcgaacgcgg cgacgaggag ggcgatgagg atgaggccgc cgatgacggc ccaccagagg
     9541 tgcccgaagc cgagcgctcg cgcgacgacg aggcagaggg gcgagaggag gacgacgatg
     9601 ccgagcgtct tgatcggctt gggaaggcgg tagaggaggt cgagaagctc gtacatgggg
     9661 ccctcagatc actcccatcg cctcggccac gctcttgacc tggcccacca gcgtggccca
     9721 ggccacgaac gtcagtatcc agaacccgat caggacgctg aagctgacga tggcgacgcg
     9781 gccgagcgtc acgacggggt tcagcctctt ccccgacgag gccatcaccg tgtacgcctc
     9841 gggcgtgatc ttgtcgctct ggctcgcgag gtattccgag aggatccggt aggtgttctt
     9901 cctcacctcg gcgagcttct cgggccgctc ccggtacttg ccgcggaagc cgagcgcgag
     9961 cccggcgtag acgatcgagc cgagctcgac gtcctccggc cggagctcct tcgcgatcgc
    10021 gaagtactgg tcgccgccga tgttcgagcg gaagtacttc tcctcgag
//
LOCUS       DAOTEZ010000119        10028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4745_length_10028_cov_5.514389, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000119 DAOTEZ010000000
VERSION     DAOTEZ010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10028
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4745_length_10028_cov_5.514389"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /locus_tag="P5164_07955"
     CDS             <1..1372
                     /locus_tag="P5164_07955"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRY43864.1"
                     /translation="SYRQPVPIRIVVPDPKTTLSWTGAAATAPAGRFGDDFVLAFPRP
                     ESAGSVSADVVFGGYGIEAKEWGWNDYEGSDVRGKLLLLLSGEPGGDDPALFAGPTLT
                     HHGRWRTKLEVAAKRGAAGVVFVHTREGAGYGWEVVRNGWTRTVAYAPDAAALSLEGW
                     LTEAEATRAVRAGGVTLESLREAAGKRGFRPIPLALKLEAHAAPRYESVVSGNVAGIV
                     PGKGPAGSAPVVVVSAHHDHLGVGTPEKGDGIYNGAMDNGSALAVLLALSRRIAARAG
                     ELPVDVLFLAPAAEEAGLLGSDLFARTPPVPLARIAANLNLEMSAVWGPARDVVAIGG
                     SESALGAVVEAVAKREGLRIAPEPAPDQGFFYRSDQFSFAKAGVPGIWIDLGDDLVGT
                     PPGTGLARREEYRAKRYHRPSDELDPSWELTGTAQLARLVELAIEEIGARNGKVPWRT
                     GSPYAR"
     gene            1394..3145
                     /locus_tag="P5164_07960"
     CDS             1394..3145
                     /locus_tag="P5164_07960"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRY43865.1"
                     /translation="MSDTPERPAEGGEAEQPGFVSLGLDPRLLAALSALGYEEPTPVQ
                     RQAIPILLEGRDLLAQAATGTGKTAAFALPMLDRLVREGGERRGVFGLVLVPTRELAM
                     QVAEAVHRYGKQAGVRVLAVYGGSSMGLQLRALERGVDVVVATPGRALDHVRRGTLAL
                     ADVKLVVLDEADEMLDMGFAEDLEAILDATPDGRPMALFSATFPPRIAVLAARRLSSP
                     TELKLGASRAMGTAPEGQGRVKQTAYVVPRAHKLAALSRVLDLEDPTSAIVFCRTRTE
                     VDEISEKLNARGRRAEALHGGFSQEQRDRVMKRFRSGSADLLVATDVAARGLDVEQVS
                     HVFNFDVPSEAEAYVHRIGRTGRAGRAGTAITFYEPREQWLLANVERLTKKKIERATV
                     PTVADLKARRLELTRASLRETILGGGLDTFRVAVESLADEFDLLDVAAAGVKLAHEAA
                     GSPGEEEEIPAIPVPPERPRFAPRGDGPAPGPFPARRGPRLAGAVRLFVGAGRDAGIR
                     PADLFGAITNEAKLPSRAIGAIEIADRFSLVEVPGESADAVIEALRRTTLRGRKVLVR
                     RERPMPPRRPFPPRGEG"
     gene            3310..3591
                     /locus_tag="P5164_07965"
     CDS             3310..3591
                     /locus_tag="P5164_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HRY43866.1"
                     /translation="MATKKEKPLAERFAEAKGRVEQLTKRPTNDQLLELYAFYKQATE
                     GDVAGSRPGLLDLKGRAKYDAWGKVKGTGKDEAMKKYVALVDALRAELG"
     gene            3593..4246
                     /locus_tag="P5164_07970"
     CDS             3593..4246
                     /locus_tag="P5164_07970"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRY43867.1"
                     /translation="MIFLDELRAEHVLIDRVAASLRSFAAARARGEGDASDGAAFRRF
                     LRLWAGAWHHAREEETLFPALAEKAELALERGPLAAFVEQHQDMAAQIDAFDPLLDRP
                     VMTEEEGRRLIALVARYTAELGRHIDAENSVLIPESAVRLAQVNVLELHGRTPSAEEL
                     AARAEGERLAAAWPAAPDPGVRGEGCVVCPSFGTTCRGVEAEWWNESEWEEFSDHLG"
     gene            4446..5036
                     /locus_tag="P5164_07975"
     CDS             4446..5036
                     /locus_tag="P5164_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43868.1"
                     /translation="MASDTTNYLAVSKEAVAWVKAMVPFGASNRPSDYLESWKKNRKK
                     IEGPDPGVLDTVLGGMTAFLPLLTGANRAMQIKEEVTGEAASDYLSRFRQIYEITLRT
                     KAGNCNEQSITAFVWLLDRGVRPLAWMHLTNGKHAFVVVGRKPGSGDDPTKWGDACVV
                     CDPWAGEAYSLPAVQAGAILQTRWGCGTAKAVFGVA"
     gene            complement(5040..6767)
                     /locus_tag="P5164_07980"
     CDS             complement(5040..6767)
                     /locus_tag="P5164_07980"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HRY43869.1"
                     /translation="MNADLTAIARAWRDADPDPETANEIDRLLEARDEAALEDRFRAE
                     LEFGTAGLRGLLGAGPNRMNRAVVRRATAGVARHLLATVPDAGRRGVVLARDARRGSD
                     SFALETAGVLAGHGIRVFSFVDRAPTPLGAFAVKELGAAAGVVVTASHNPPEYNGYKV
                     YWENGAQIIPPFDAGIAGEIAKAGAANALPFLPRAEAEARGLFVPLGEEMEERYHAAI
                     AGLALHPGEGRDLGIVYTAMHGVGARFTLAALARAGFTSVHPVAAQNEPDGAFPTVAF
                     PNPEEKGALDLSLALCEETRADLLLANDPDADRLAAGARDRQGRMRVFSGNEIGVLLG
                     AYCLKERRPKPERPLVVTTIVSSSQLGKIARDLGAASAETLTGFKWIENRALELERTE
                     GRTTVFGYEEALGYSVGDVVRDKDGISAALVFADLAGWCRARGVTAWDYLEEIQREHG
                     LFLSAQRSVTIPGAEGAATIAAIMDGFRARPPAAVGGLAVAETRDYRAGVPGLPKSNV
                     VAYLLDGGSRVTLRPSGTEPKIKYYFELVEAPRAGEPIPSAIVRGEERLAALVDDFVR
                     LAVDRGQPA"
     gene            complement(6793..8610)
                     /locus_tag="P5164_07985"
     CDS             complement(6793..8610)
                     /locus_tag="P5164_07985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRY43870.1"
                     /translation="MTGPRILLPCLFLSAFAVHGSPAVAQAAPPAAAPQTPPRTTASV
                     ELSLTNLDVVVTDAKGNPVHGLLATDFEVLHDGRPVEVTNFSEIRLEGAAVQTPATSA
                     APAVTTTPVPPATAPAARPRRRVVIFFDRLYLPEPGVRKALFDSLRSLVADTLEEGDD
                     VMIVTWERSVRTVLPFTDDLDALEPALERIERRCARPSSEVGDSALLEWETSWFKSQA
                     ADPRVGAGAGGGSETTQEIMARQAFFEMKGKTAALRGLAATLGGVEGRKALVLVTNRF
                     SRFPGLEFLIGSRPDAGEIESAKRRVQDGRKLLEEVTQAANANGVTLYGLFPDAFEGS
                     LLPSAADSPTSSPAWEHPNFDYSNVGGGRGAPAAPARTLPPASGPALGVRRDALLQNE
                     VEALDVVASATGGVALVGSGNVGRLVERVSSDLQSWYSLGYPSRPGTGRTASVSVRVK
                     GRDLTVRARRAIVEKSVEEQMSDRVLAHLFQPDERSRIPISASLVGSTPGKGKHVLRL
                     EVRIPIESLALLPTAKGVQGVFSVFVASVAAEGDFSTVTRLNQPFEIPAKDLETAQAG
                     HYTYELDVVTTGPGARVAIGIWDEKSNEAGFAVVTPPRS"
     gene            8681..10009
                     /locus_tag="P5164_07990"
     CDS             8681..10009
                     /locus_tag="P5164_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43871.1"
                     /translation="MVSFVSLFVGFVVGIVNVQLMATAEVHRVELFLDGQVVAELREP
                     YSRPVDLGCEPAPHELVAVAYDERGRVLSSARQWVNRPRATADLSLLVDGGGESPPRT
                     ARLVWRALAGEVPRSIAVSFDGVMLPVTNPQRIELPEHDPTHVHLLRADIDFGRGVVA
                     SAEMIVGGPSRVEARSELSAIPLSVDEGAALPAAEDLAGWLEAGGRPLEVAAVEDGPV
                     DVVVVGERSVPEALKRFGRRSRRSRRSRAESTATAPAMPSEAPAPVALDVRLRFLWPV
                     ATIWTQSAMVANNFLASSSSDEARLDIDWLKARHGDWPNWSGTSERLADAVAVAALTA
                     AEGNRRRAVVLVLGPSAKDDSLVTAEETARFLGRIGVPLYVWSLGETPSPESSRWPAP
                     RTIASAADLDTAVRDLTTAAARQRIVWVEGSHLPQDVELGPLAKGIRLAR"
BASE COUNT         1342 a         3489 c         3859 g         1338 t
ORIGIN      
        1 gagctaccgc cagccggtcc cgatccgcat cgtcgtgccc gatccgaaga cgaccctctc
       61 gtggacgggc gcggcggcca cggccccggc cggccggttc ggcgacgact tcgtcctcgc
      121 gttcccgcgc ccggagtcgg cggggagcgt ttcggccgac gtcgtcttcg gcggctacgg
      181 aatcgaagcg aaggaatggg gctggaacga ttacgagggg tccgacgtcc gggggaagct
      241 gctcctcctg ctgtcgggcg agccgggcgg cgacgacccg gccctcttcg ccggcccgac
      301 cctgacgcat cacggccggt ggcggacgaa gctcgaggtc gccgcgaagc gcggcgccgc
      361 gggggtcgtc ttcgtccaca cgcgcgaggg ggccggctac ggctgggagg tcgtccggaa
      421 cggctggacg aggaccgtcg cgtatgcccc cgacgcggcg gccctctcgc tcgagggctg
      481 gctgacggag gcggaagcca cgcgcgccgt gcgggcgggg ggcgtgacgc tcgagtcgct
      541 ccgcgaggcc gccgggaagc ggggcttccg gccgatcccg ctcgccctga agctcgaggc
      601 gcacgccgcg ccccgctacg agtcggtcgt ctcggggaac gtcgccggga tcgtccccgg
      661 gaaggggccg gcgggctcgg ctccggtcgt cgtcgtgagc gcccaccacg accacctcgg
      721 cgtcgggacg cccgagaagg gcgacggcat ctacaacggc gcgatggaca acggctcggc
      781 gctcgcggtg ctcctcgcgc tctcgcggcg gatcgcggcc cgggcggggg agcttccggt
      841 cgacgtcctc ttcctcgcgc cggcggccga ggaggcgggc ctcctcggct cggacctctt
      901 tgcccggacg ccgccggtgc cgctcgcgcg gatcgcggcg aacctgaacc tcgagatgtc
      961 ggccgtctgg ggcccggcgc gcgacgtcgt cgccatcggc ggctccgaga gcgccctcgg
     1021 cgcggtcgtc gaggcggtgg cgaagaggga gggcctccgg atcgcgcccg agccggcgcc
     1081 cgaccagggc ttcttctacc gctcggacca gttctccttc gcgaaggcgg gagtgcccgg
     1141 gatctggatc gacctgggcg acgacctcgt cgggacgccg cccggcacgg gtctcgcgcg
     1201 gcgcgaggag taccgcgcga agcgctacca ccgcccgagc gacgagctcg atccgtcgtg
     1261 ggagctgacc gggacggcgc agctggcgcg gctcgtcgag ctcgccatcg aggagatcgg
     1321 cgcccgcaac gggaaggtcc cctggaggac cggctctccg tacgcccgct gaccgagggg
     1381 gtagcatgag ggcgtgagcg acacgcccga acgacccgcc gaaggcgggg aagccgaaca
     1441 gcccggcttc gtctccctcg gcctcgaccc ccgcctcctc gccgcgctct ccgccctcgg
     1501 ctacgaggag cccacgcccg tccagaggca ggcgatcccg atcctcctcg aggggcgcga
     1561 cctcctcgcg caggctgcga cgggcacggg caagacggcc gcgttcgcgc ttccgatgct
     1621 cgaccggctc gtgcgggagg ggggagagcg ccggggcgtc ttcggcctcg tcctcgtccc
     1681 gacgcgcgag ctcgcgatgc aggtggccga ggcggtccac cgctacggga agcaggccgg
     1741 ggtgagggtc ctcgccgtct acggcggctc ctcgatgggc ctgcagctcc gggcgctcga
     1801 gcgcggcgtg gacgtcgtcg tggcgacgcc gggccgggcg ctcgaccacg tccggcgcgg
     1861 gactctcgcg ctcgccgacg tgaagctcgt cgtcctcgac gaggcggacg agatgctcga
     1921 catgggcttc gcggaggacc tcgaggcgat cctcgacgcg acgcccgacg ggcgtccgat
     1981 ggctctcttc tcggccacct tcccgccgcg catcgcggtg ctggccgcgc gccggctctc
     2041 ctcgccgacg gagctgaagc tcggagcgtc ccgcgcgatg ggaacggccc cggagggcca
     2101 ggggcgggtg aagcagacgg cctacgtcgt gccgcgggcc cacaagctcg ccgcgctttc
     2161 gagggtcctc gacctcgagg acccgacctc cgcgatcgtc ttctgccgga cccggaccga
     2221 ggtcgacgag atctcggaga agctgaacgc gcggggccgg cgcgccgagg cgctccacgg
     2281 cggcttctcc caggagcagc gcgaccgcgt gatgaagcgg ttccgctcgg gctccgcgga
     2341 cctgctcgtg gcgaccgacg tggcggcccg cggcctcgac gtggagcagg tctcgcacgt
     2401 cttcaacttc gacgtcccga gcgaggcgga ggcctacgtc caccggatcg ggcggacggg
     2461 gcgggcgggt cgcgccggga ccgcgatcac gttctacgag ccgcgcgagc agtggctcct
     2521 cgcgaacgtc gagcggctca cgaagaagaa gatcgagagg gcgacggtgc cgaccgtcgc
     2581 cgacctgaag gcgcggcgcc tcgagctgac gcgcgcctcg ctccgggaga cgatcctggg
     2641 gggcggcctc gacacgttcc gcgtcgcggt ggagtcgctt gccgacgagt tcgacctcct
     2701 cgacgtcgcg gcggcgggcg tcaagctggc gcacgaggct gcggggtcgc cgggggagga
     2761 agaggagatc ccggcgatcc ccgttccgcc ggaacggccc cgtttcgcgc cgcgcggcga
     2821 cggtcccgct ccgggtccct tcccggcccg gcgcgggccg cggctcgcgg gagcggtgcg
     2881 cctcttcgtc ggggccggcc gcgacgcggg gatccgtccc gccgacctct tcggcgccat
     2941 cacgaacgaa gcgaagctcc cgtcgcgcgc gatcggggcg atcgagatcg ccgaccgatt
     3001 ctcgctcgtg gaggtgcccg gggagtcggc ggacgccgtc atcgaggcgc tccggcggac
     3061 gacgctgcgg ggacggaagg tcctcgtccg gcgggagcgc ccgatgccgc cgcgacggcc
     3121 cttcccgccg cgcggcgagg gctgaggccc gcgcgacgac cgggggcctc gccgcggcgc
     3181 ggcggagggt gggaggattc tgctatcttc gcggcacgtt tcggagacat gcgagcggaa
     3241 gtcgtgcgaa atacacgatt tcacgcctga ccccgtcggg gggggccgtc ggggctgcgg
     3301 gaggggacga tggcgacgaa gaaggagaag cctctggcgg agcggttcgc ggaggcgaag
     3361 gggcgcgtcg agcagctgac gaagcggccg acgaacgacc agctcctcga gctctacgcg
     3421 ttctacaagc aggccaccga aggagacgtc gcggggagcc gccccggcct cctcgacctg
     3481 aaggggcggg cgaagtacga cgcctggggg aaggtcaagg ggaccgggaa ggacgaggcg
     3541 atgaagaagt acgtcgcgct cgtcgacgcg ctccgggccg agctcggctg acgtgatctt
     3601 cctcgacgag ctgcgggccg agcacgtcct gatcgaccgc gtcgcggcgt cgctccggag
     3661 cttcgcggcg gcgcgcgccc ggggcgaggg cgacgcctcg gacggggccg cattccggcg
     3721 gttcctccgg ctctgggccg gagcgtggca tcacgcgcgc gaggaggaga cgctcttccc
     3781 ggcgctcgcg gagaaggcgg agctcgccct cgagcgcggg ccgctggccg cgttcgtcga
     3841 gcagcaccag gacatggcgg cgcagatcga cgcgttcgac ccgctcctcg accgcccggt
     3901 aatgacggag gaggaggggc ggcggctcat cgccctcgtc gcacggtaca cggccgagct
     3961 cggccgtcac atcgacgcgg agaactcggt cctgatcccg gagagcgcgg tccggctcgc
     4021 gcaggtcaac gtcctcgagc tgcacggacg gacgccgtcg gccgaggagc tcgcggcgcg
     4081 tgcggaagga gagcggctcg cggccgcctg gccggccgcg cccgacccgg gcgtgcgcgg
     4141 cgagggctgc gtcgtctgcc cgtcgttcgg cacgacgtgt cgcggcgtgg aggccgagtg
     4201 gtggaacgag agcgagtggg aggagttctc cgaccacctc gggtgagggt ccggggaggg
     4261 acgactccga cgcttcggtg acagggcgca cggaacccgc gtcgccgttt ccgcgtattc
     4321 gtctcagatc gagcgagcat cggcccgaag acgcgtcccg cgggacgcgg gggggcgtgt
     4381 ggctcgtcgg cggatcgaca ggagctcggg acgctctgcg ggaagcgtcg ccgaaaggag
     4441 cggacatggc ctcggacacc acgaactacc tcgccgtctc gaaggaggcc gtcgcctggg
     4501 tgaaggcgat ggtgcccttc ggagcctcga accggccctc ggactacctc gagagctgga
     4561 agaagaaccg aaagaagatc gagggcccgg accccggggt cctcgacacg gtgctcggcg
     4621 ggatgacggc gttccttccg ctcctgacgg gagcgaaccg cgcgatgcag atcaaggagg
     4681 aggtgacggg ggaggccgcg tccgactacc tctcgcgctt ccgccagatc tacgagatca
     4741 cgctgaggac gaaggcgggc aactgcaacg agcagagcat cacggccttc gtctggctcc
     4801 tcgaccgtgg cgtgaggccg ctcgcgtgga tgcacctgac gaacggcaag cacgcgttcg
     4861 tcgtcgtcgg gcggaagccc gggagcggcg acgacccgac gaagtggggt gacgcttgcg
     4921 tcgtctgcga tccgtgggcg ggcgaggcct actcgctgcc ggccgtccag gcgggcgcga
     4981 tcctccagac caggtggggt tgcggcacgg cgaaggccgt tttcggcgtc gcgtagcggc
     5041 tacgcgggct gaccccggtc gacggcgagc cggacgaagt cgtcgacgag cgcggcgagg
     5101 cgctcctctc cccggacgat cgcggacggg atcggctcgc cggcgcgcgg cgcctccacg
     5161 agctcgaagt agtacttgat cttcggctcg gtccccgacg ggcggagggt gacgcgcgag
     5221 cccccgtcga ggaggtacgc gacgacgttc gacttcggaa ggcccgggac ccccgcgcgg
     5281 tagtcgcgcg tctcggcgac ggcgaggccg ccgaccgcgg cgggcggccg ggcgcggaag
     5341 ccgtccatga tcgcggcgat cgtcgcggct ccctcggcgc cggggatcgt cacgctcctc
     5401 tgagcggaga ggaagagccc gtgctcccgc tggatctcct cgaggtagtc ccacgccgtg
     5461 acgccgcgcg cgcggcacca gccggcgagg tccgcgaaga cgagcgccgc cgagatgccg
     5521 tccttgtcgc ggacgacgtc tccgacggag tagccgagcg cctcctcgta gccgaaaacg
     5581 gtcgtccgcc cctcggtccg ctccagctcc agggcgcggt tctcgatcca cttgaagccg
     5641 gtgagcgtct cggcggaggc ggcgccgagg tcccgcgcga tcttcccgag ctggctcgac
     5701 gagacgatcg tcgtcacgac gaggggcctc tccggcttcg gacgccgttc cttcaggcag
     5761 taggcgccga ggaggacgcc gatctcgttg ccggagaaga ccctcatccg tccctgccgg
     5821 tcgcgggcgc cggcggcgag gcggtcggcg tcggggtcgt tcgcgaggag gaggtcggcc
     5881 cgcgtctcct cgcagagcgc gagggagagg tcgagcgccc ccttctcttc ggggttcggg
     5941 aacgcgacgg tggggaaggc gccgtcgggc tcgttctggg cggcgaccgg atgaacggag
     6001 gtgaagccgg cgcgggcgag cgcggcgagg gtgaagcggg cgccgacgcc gtgcatcgcc
     6061 gtgtagacga tgccgaggtc ccgtccctct cccgggtgga gggcgaggcc ggcgatcgcc
     6121 gcgtggtaac gctcctccat ctcctcgccg agcgggacga agagcccccg cgcttcggcc
     6181 tccgcgcgcg ggaggaaggg gagggcgttc gccgctccgg ccttcgcgat ctctccggcg
     6241 atcccggcgt cgaagggcgg gatgatctgg gcgccgttct cccagtagac cttgtagccg
     6301 ttgtattcgg gcgggttgtg gctggccgtg acgacgaccc cggccgccgc cccgagctcc
     6361 ttcacggcga aagcgccgag cggcgtcgga gcgcggtcga cgaaggagaa gacgcgaatg
     6421 ccgtgaccgg cgaggacgcc ggccgtctcg agcgcaaacg agtcgctccc gcgccgcgcg
     6481 tcgcgggcga ggacgacgcc gcgccggccg gcgtcgggga cggtcgcgag gaggtggcgg
     6541 gcgaccccgg ccgtcgcgcg cctcacgacg gcgcggttca tgcggttcgg cccggcgccg
     6601 aggagcccgc ggaggccggc cgtgccgaac tccagctccg cccggaagcg gtcttcgagg
     6661 gccgcttcgt cgcgcgcttc caggaggcgg tcgatctcgt tcgctgtttc ggggtcgggg
     6721 tcggcgtccc gccaggcgcg ggcgatcgcg gtcaggtcgg cattcatccg ggagatggta
     6781 gcgccccgcg gctcaggagc gcggcggcgt gacgacggcg aagccggcct cgttcgactt
     6841 ctcgtcccag atgccgatcg cgacccgggc gccagggccc gtcgtcacga cgtcgagctc
     6901 gtaggtgtag tgcccggcct gggccgtctc gaggtccttc gccgggatct cgaacggctg
     6961 gttcaggcgc gtcaccgtcg agaagtcccc ttccgcggcc accgacgcca cgaagaccga
     7021 gaagaccccc tggacgccct tcgcggtcgg caggagcgcg agggactcga tcgggatccg
     7081 cacctcgagc cggaggacgt gcttcccctt ccccggcgtc gagccgacga ggctcgccga
     7141 gatcgggatg cgcgagcgct cgtcgggctg gaagaggtgc gcgaggaccc ggtccgacat
     7201 ctgctcctcg acgctcttct cgacgattgc gcggcgggcc cggaccgtca ggtcccgccc
     7261 cttgacgcgg acggacaccg acgcggtgcg ccccgtgccg ggcctggacg ggtagccgag
     7321 cgagtaccac gactgcaggt cgctcgagac ccgctccacg aggcggccga cgttcccgga
     7381 gccgacgagg gcgacgcccc ccgtcgccga ggcgacgacg tcgagcgcct cgacctcgtt
     7441 ctgaaggagg gcgtcccgcc ggacgccgag ggcggggccg ctcgccgggg gaagcgtccg
     7501 cgccggtgcg gcgggggcgc cccggccgcc gccgacgttg ctgtagtcga agttcgggtg
     7561 ctcccaggcc ggggaggacg tcggggaatc ggccgcggac gggaggagcg acccctcgaa
     7621 ggcgtcggga aagaggccgt agagcgtgac gccgtttgcg ttcgcggcct gggtgacctc
     7681 ctccaggagc ttccggccgt cctgcacgcg ccgcttcgcg ctctcgatct ctcctgcgtc
     7741 ggggcgggac ccgatgagga actcgaggcc ggggaagcgg gagaagcggt tcgtgacgag
     7801 gaccagcgcc ttccggccct ccacgccccc gagcgtcgcc gcgaggcccc tcagagcggc
     7861 cgtcttcccc ttcatctcga agaacgcctg ccgcgccatg atctcctgcg tcgtctcgct
     7921 tccgcctccg gcgccggccc cgacccgcgg gtcggccgcc tgggacttga accaggaggt
     7981 ctcccactcg aggagcgccg agtcgccgac ctccgagctc ggccgggcgc agcggcgctc
     8041 gatccgctcc agggcgggct ccagggcgtc gaggtcgtcg gtgaacggca ggacggttcg
     8101 gaccgagcgc tcccaggtga cgatcatgac gtcgtccccc tcttcgagcg tgtcggcgac
     8161 gagggaccgg agcgaatcga agagggcctt ccggacgcca ggctccggaa ggtagagccg
     8221 gtcgaagaag atgacgacgc ggcggcgggg gcgcgccgca ggcgccgtgg ccggcgggac
     8281 gggggtcgtc gtgaccgccg gtgcggcgga ggtcgcaggc gtctgaacag cggcgccctc
     8341 cagccggatc tcgctgaagt tcgtcacctc gaccgggcga ccgtcgtgga ggacctcgaa
     8401 gtcggttgcg aggagcccgt ggacggggtt ccccttcgcg tcggtcacga cgacgtcgag
     8461 gttcgtgagg ctcagctcga cggaggcggt cgttcgggga ggggtctgtg gggcagcggc
     8521 cggaggagcg gcctgggcca ccgcgggcga gccgtgaacc gcgaacgcgg agaggaagag
     8581 gcacgggagg agaattcgcg gacccgtcat ggtgttcctc cggcacgcag gatgcgtgcc
     8641 gcgagcgagt gtacccttca ggggtcgacg ggaagggatc atggtctcgt tcgtttcgct
     8701 cttcgtcggg ttcgtcgtcg ggatcgtcaa cgtccagctc atggcgaccg ccgaggtcca
     8761 ccgggtcgag ctcttcctcg acggccaggt cgtcgcggag ctgcgcgagc cgtactcgcg
     8821 gcccgtcgac ctgggctgcg agccggcgcc gcacgagctc gtcgccgtgg cctacgacga
     8881 gcgcggacgg gtgctctcct ccgcccggca gtgggtgaac aggccgcggg cgacggccga
     8941 cctgagcctg ctcgtcgacg gcggcgggga gtccccgccg cggaccgcgc ggctcgtctg
     9001 gcgcgccctc gcgggggagg tcccccgttc catcgcggtc tcgttcgacg gggtgatgct
     9061 gccggtgacg aacccgcaac ggatcgagct tccggagcac gacccgacgc acgtccacct
     9121 ccttcgcgcc gacatcgact tcgggcgagg cgtggtcgca tcggccgaga tgatcgtcgg
     9181 cgggccgtcg cgggtcgaag cgcgctcgga gctctccgcc atcccgctgt cggtcgacga
     9241 aggcgccgcc cttcccgcgg cggaggacct cgccggttgg ctcgaagccg gcgggcgtcc
     9301 gctcgaggtc gcggccgtcg aggacggccc cgtcgacgtc gtcgtcgtgg gggagcgaag
     9361 cgtgccggag gccttgaagc gattcggccg ccgcagccgg cgctcgcggc gaagtcgcgc
     9421 cgaatcgacg gcgacggccc cggcgatgcc gtcggaagct ccggcgcccg tcgccctgga
     9481 cgtccggctc cggttcctct ggcccgtcgc caccatctgg acgcagtccg ccatggtggc
     9541 gaacaacttc ctcgcgagct cgtcctcgga cgaggcgcgg ctcgacatcg actggctgaa
     9601 ggcgcggcac ggcgactggc cgaactggag cgggacgtcg gagcggctgg cggacgccgt
     9661 cgcggtggcg gccctgacgg ccgcggaggg gaaccgccgc cgcgcggtgg tcctcgtcct
     9721 cgggccctcc gcgaaggacg acagcctcgt gacggcggag gagacggcgc gcttcctcgg
     9781 acggatcggc gttccgctct acgtctggtc gctcggcgag accccgtccc ccgagtcgtc
     9841 ccggtggccc gcgccgcgga cgatcgcgag cgcggccgat ctcgacaccg ccgttcggga
     9901 cctcaccacc gcggcggccc gccagcggat cgtctgggtg gagggatctc acctgccgca
     9961 ggacgtggag ctcgggccgc tcgcgaaggg gatccggctc gcgcgctgag gccccgatgc
    10021 cggtgcga
//
LOCUS       DAOTEZ010000120         9991 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4763_length_9991_cov_5.412943, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000120 DAOTEZ010000000
VERSION     DAOTEZ010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9991
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4763_length_9991_cov_5.412943"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="P5164_07995"
     CDS             complement(<1..1027)
                     /locus_tag="P5164_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRY43872.1"
                     /translation="MPTNVIMPQMGESIAEGTLTRWMKKVGDTVKRDEPLFEISTDKV
                     DAEIPAPAAGVLLEILVTEGTTVPINTVVAKIGEAGEATSSASEAPAPAPTAVAPAPP
                     AAPAAVPAPAPAPAPAPAPAPAPAPAPAAPHFAVPPQPGETKEELQKRRSSPVVRNIA
                     EEKGVDLAQVPGSGISGRVTKADITAFLEKGGTPPPAPAPAVAAPAPTVATPAPAAAV
                     PASAPAVAAAPPPAPAKPAPAAPATPVISFPAGANVTVEPMSTMRKKIAQRMVESKQT
                     SAHVATVFEVDYTSIARLRDRVKERFAAQYGTKLSYMPFIFKAVIAGLKARPAVNASV
                     SGDDVLYH"
     gene            complement(1058..2047)
                     /locus_tag="P5164_08000"
     CDS             complement(1058..2047)
                     /locus_tag="P5164_08000"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HRY43873.1"
                     /translation="MIAAPSGEVTLLDAVRIALAEEMERDGRVVLMGEDIGVYGGAFK
                     ATAGLLERFGPKRVIDTPVVEEAIVGAAIGASLRGLRPVVEMQFFDFASRAFDMITNF
                     AAKHRYRTGLGVPIVVRGPSGGGVQAGPFHSQSPEGYFARTPGLAVVVPSTAQDARGL
                     LKAAIRDDDPVLFLEHKLLYRRAKGTLPEGDGSVPIGKAAVRREGRDLTIVTWGAMVP
                     LALDGAAELAREGVEAEVIDLRTLLPMDEGTVLESVRKTSKALVLHEESRTLGVGAEA
                     AALLAEKAFEWLDAPVVRVTAPDTPVPFAPALEAAYLPGPATLLAAARKLAAY"
     gene            complement(2044..3057)
                     /locus_tag="P5164_08005"
     CDS             complement(2044..3057)
                     /locus_tag="P5164_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HRY43874.1"
                     /translation="MTFSLEQQRSLYRHLLLTRLLEERMVSLYRQGKMTGGLYRCLGQ
                     EATAVGTAFALEEGDLLIPVIRDLGSVLVRGARPADVLRQLLAKGDGPSRGRDGFLHF
                     CLPRRGLYSGIAMLGTAVPVLAGMVLADRFLGKRTVGMTYLGEGGTSTGAFHEGINFA
                     GVQRLPVVVVLEYNHYSYSTPSEMQCAAATLADKAAGYGISSAIVDGNDVLSVVEAAR
                     RAVAHARAGNGPFLLECKTYRRKGHAEHDGQAYVDAGELAEWARRDPIALFEKQLDAS
                     GVMDAAARAAVRAEVDAELDAAVAEAEASPAPVPDPEFRGVRADEEDAVRRRRETFVG
                     GLA"
     gene            3125..4435
                     /locus_tag="P5164_08010"
     CDS             3125..4435
                     /locus_tag="P5164_08010"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HRY43875.1"
                     /translation="MTRRPPKTSSLGRLLELGGLAVRAGTSTAASFVSSLGRPAARRE
                     ELRIEELVRNARRLVATLGEMKGAAMKVGQMLSLQDAILPPEVAAVLRALQKESPSLP
                     LEMVEEQLAEELGDPLQLFVSFEPEAFAAASIGQVHRAVLRDGRRVAVKIQYPGIDRM
                     VEADLGNLRRVLKSVVALVSKVDFEPIWQELRARLREELDYLHEAERMRRMAELHADV
                     PEVVIPRVVEEASTGRILTTEYEEGLSPDEACAPETPQELKDRWGAVLFDFLLRGLLR
                     HRLLHADPNLANFSFRPDGRVVVYDFGCVKEVPLRLARGYRGLCRAALEGRTDDLPDV
                     LKRMGMHRLDGSPLEERLITPWAALVLELLRPAPPYRFGSDDSVARRLLDAARTSLAE
                     AGDVRFPRDIVFVNRTVLGHFGNLARLRAEGPWRDILGRFTEKE"
     gene            4492..>5176
                     /locus_tag="P5164_08015"
     CDS             4492..>5176
                     /locus_tag="P5164_08015"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY43876.1"
                     /translation="MSNTPGSSYDEVPYVDTCHAATHPDRLATLAAVYGLPAPSLERC
                     RVLELGCARGGNLLPMAMELPEASFVGVDLSARQVAEARERAEALGLRNVAFHAMGLE
                     EIDAGFGTFDFVVCHGVYSWVPEPVRERILEICATNLSAEGLAYVSYNTYPGWSSRGM
                     VRDLVRFHARSEETPEGRVERARGFLEELAGHVVRQEGAYASVLKVEASRFREADDTY
                     LLHEFFEDDN"
     assembly_gap    5177..5276
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5277..6230
                     /locus_tag="P5164_08020"
     CDS             <5277..6230
                     /locus_tag="P5164_08020"
                     /inference="COORDINATES: protein motif:HMM:NF021624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HRY43877.1"
                     /translation="FREADDTYLLHEFFEDDNAPCFVHEFLRRAEAAGLEFVAEAQAP
                     GLFDGLPESARELVSRWAGGVAAREQYLDLMVNRTFRRTVLRRAGGPPAVAPAPEVVE
                     RLAARAVVVPSPVAWDAEGKDGPIEFVHPGRSGSIQTNDPALKAALLALHEARPAALP
                     FETLWKRVREGLAVRGAPSPRPARETREALRSSLLRLFLADFVELHVRPPRPATDPAE
                     RPVGSPFARRQAADGNRVTSLARRRVELDELARAVLLELDGAHTEAEVLDAVVARVCS
                     GSLELSDESGPVREAAAARALLAGEIRPAIERLARLALLAG"
     gene            6332..7774
                     /locus_tag="P5164_08025"
     CDS             6332..7774
                     /locus_tag="P5164_08025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter"
                     /protein_id="HRY43878.1"
                     /translation="MIAVFVLYMVATVSLAFWYSRGARSHRDFVLGGGRFGGTALALS
                     ERATGESAWLLLGLTGHAYADGLSTIWVALGCVLGILFIWMVMARRLREETERTGAMT
                     VSSLLARRFPGSEKPIALLSAGIVIFFFMLYIAAQFSGSGKVLQETFGLDPLWGILIA
                     SVVVTIYCGLGGFIAVVVTDVFQALLMIFTLVAFPVIALVLAGSKGIDLAAAFAAAGP
                     SYLSLTGGKTGAAAVILVLSGLSWALGYTGQPQLLTRMMAIRSEKDVKTAVRVASVWT
                     LFAYAGAILIGMIGVAFVQSGAVGGGVGKLADAEKVLPAMVTTLVTPILAGVLLSGVI
                     SAMMSTASSEVTVSSASFSEDVVAMLRKKAATPRGMLLLNQGATVAVGAIAIVLALTM
                     RDTVYSLVSYAWSGIGSSFGPALMLLLFWKRLSRAGVFASLVTGTVATVVWKSFLEQP
                     TGVSERLASYVVALAMAVAFSLAFPEKRRT"
     gene            7802..9382
                     /locus_tag="P5164_08030"
     CDS             7802..9382
                     /locus_tag="P5164_08030"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY43879.1"
                     /translation="MANDTGRSYDEVPYGEGCFRVSQPDHLAALAAVHRIPAPPLERC
                     RVLELGCGSGGNLLPMALELPEATFVGVDLSEGQIASARERASALGVRNADFRAMDLA
                     AIDASFGTFDYVVCHGVYSWVPEAVQERILAICSANLSPDGLAYVSYNTFPGWHERRM
                     ARELMLYHDRAGGPSAERIARARSFPGELARVVLSPESAYGRVLRAEAEALKDEEDAY
                     VFHEFLEDVNRPTYVHELLTRAARHGLEFVTEAGTPGLFAGLPAPSREAVERWAVDVI
                     SREQYLDFVCNRPFRKTVLRHAGGPPAGAPDPAALAGLEVSSDLKPATGSPVVADESP
                     ARFTRPDVEGGASTTSPLLKAILLALDAARPRALDPAALHAGVAARLAEAGRALPADR
                     EEAEAQLRGAVLLLFLSDLVELHVRAPRPAAGVSARPLASPLARHQASRSTRVTSLRR
                     RVVEVGELERAVLLELDGRRDLDGVLDRVTARVLAGEVELSLEGKTVHDPAVVRAALA
                     VEIEPAVRALVEKALLAG"
     gene            9622..>9991
                     /locus_tag="P5164_08035"
     CDS             9622..>9991
                     /locus_tag="P5164_08035"
                     /inference="COORDINATES: protein motif:HMM:NF013298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRY43880.1"
                     /translation="MTNPKQDGRIPVAVLGATGAVGQRMVSLLAGHPTLKLAEVAASE
                     RSQGKSYLEATRWILPGDVPEEARGLVVKGVGGPLESRLVLSALDAAVADTVEPLQAS
                     RGKLVVSNTKSFRMQADVPLL"
BASE COUNT         1255 a         3551 c         3663 g         1422 t
ORIGIN      
        1 ggtggtagag gacgtcgtcg ccggagaccg aggcgttcac ggcggggcgg gccttcagcc
       61 cggcgatgac cgccttgaag atgaacggca tgtaggagag cttcgtcccg tactgcgcgg
      121 cgaagcgctc cttcacgcgg tcgcggaggc gggcgatgct cgtgtagtcg acctcgaaga
      181 cggtcgcgac gtgagcggac gtctgcttcg actcgaccat ccgctgtgcg atcttcttgc
      241 gcatcgtcga catcggctcg acggtgacgt tcgcgccggc ggggaaggag atgacgggcg
      301 tggccggggc ggccggggcc ggcttcgcgg gtgcgggcgg cggggccgca gcgacggccg
      361 gcgccgatgc ggggaccgca gcggccgggg ccggcgtcgc gaccgtgggc gccggagccg
      421 cgacggccgg ggccggcgcg ggcggtgggg tcccgccctt ctcgaggaag gccgtgatgt
      481 cggccttcgt cacgcggccc gatatgccgg agccggggac ctgcgcgagg tcgaccccct
      541 tctcctcggc gatgttcctc acgacggggg aggagcggcg cttctgcagc tcctccttcg
      601 tctcgcccgg ctgcggcggg acggcgaagt gcggcgcggc gggggccgga gcgggggccg
      661 gagcgggagc cggagcggga gccggagcgg gagccggagc cgggaccgca gccggcgcgg
      721 ccggcggagc aggcgccacc gcggtcggag ccggggcggg ggcctcggag gccgagctcg
      781 tcgcctcgcc cgcctcgccg atcttcgcca cgacggtgtt gatcgggacc gtcgtcccct
      841 ccgtgacgag gatctcgagg aggacaccgg cggcgggggc ggggatctcg gcgtcgacct
      901 tgtcggtcga gatctcgaag aggggctcgt cgcgcttgac ggtgtccccg accttcttca
      961 tccagcgggt cagcgtccct tcggcgatcg actcgcccat ctgcggcatg atcacgttcg
     1021 tcggcatggt ccgattctcc gtctacccgt ggtcgggtca gtaagccgcg agtttacgcg
     1081 cggcggccag aagcgtcgcg gggccgggca ggtacgcggc ctccagggcg ggggcgaagg
     1141 gaacgggcgt gtcgggggcg gtcacccgga ccacgggcgc gtcgagccac tcgaacgcct
     1201 tctccgcgag gagggccgcg gcctcggctc cgacgccgag ggtccgcgac tcctcgtgga
     1261 ggacgagcgc cttggaggtc ttccgaaccg actcgaggac cgtcccctcg tccatcggga
     1321 ggagcgtccg gaggtcgatc acctccgcct cgaccccttc ccgggcgagc tcggcggcgc
     1381 cgtcgagcgc gagcgggacc atcgctcccc aggtcacgat cgtcaggtcg cggccttccc
     1441 gcctcacggc ggccttgccg atcgggacgc tcccgtcccc ctcggggagg gtccccttcg
     1501 cccgccggta gaggagcttg tgctcgagga agaggaccgg gtcgtcgtcc cggatcgcgg
     1561 ccttcaggag gcctcgagcg tcctgggcgg tcgacgggac gacgaccgcg aggcccggcg
     1621 tccgggcgaa gtacccctcc gggctctggg agtggaacgg gcccgcctgg acgccgcctc
     1681 ccgaggggcc ccgaacgacg atggggaccc cgaggccggt ccggtagcgg tgcttcgccg
     1741 cgaagttcgt gatcatgtcg aacgcccgcg aggcgaagtc gaagaactgc atctcgacga
     1801 cgggacggag gccccggagc gaggcgccga tcgccgcgcc gacgatcgcc tcctcgacga
     1861 cgggcgtgtc gatcacgcgt ttcgggccga agcgctcgag gaggccggcc gtcgccttga
     1921 acgcgccgcc gtagaccccg atgtcctcgc ccatcaggac cacgcgcccg tcgcgctcca
     1981 tctcctcggc gagcgcgatc cggaccgcgt cgaggagcgt cacctcgccg gaaggggccg
     2041 cgatcacgcg agccccccga cgaacgtctc tcgccggcgc ctcacggcgt cctcctcgtc
     2101 ggccctcacg ccccggaact ccgggtcggg gacgggggcg ggggaggcct cggcctcggc
     2161 gaccgcggcg tcgagctccg cgtcgacctc cgcgcggacg gcggcccggg ccgcggcgtc
     2221 catcacgccg ctcgcgtcga gctgcttctc gaagagggcg atcgggtcgc gccgcgccca
     2281 ctcggcgagc tccccggcgt cgacgtaggc ctgcccgtcg tgctcggcgt gccccttccg
     2341 gcggtaggtc ttgcactcga ggaggaacgg gccgtttccc gcgcgagcgt gggcgacggc
     2401 gcgccgcgcc gcctcgacga cggagaggac gtcgttcccg tcgacgatcg cgctcgagat
     2461 gccgtacccg gcggccttgt cggcgagcgt ggcggcggcg cactgcatct cggacggggt
     2521 cgagtaggag tagtggttgt actcgaggac gacgacgacg ggcagtcgct ggacgcccgc
     2581 gaagttgatc ccctcgtgga acgcccccgt ggacgtccct ccctcgccga gataggtcat
     2641 cccgacggtc cgcttgccca ggaatcgatc ggcgaggacc atccccgcga ggacgggcac
     2701 cgccgtgccg agcatggcga tcccggagta gagccccctc cggggaaggc agaagtggag
     2761 gaacccgtcg cgcccccgcg aggggccgtc tcccttcgcg aggagctgcc ggaggacgtc
     2821 cgccggccgc gctccccgca cgagcaccga gccgaggtcg cggatcaccg ggatcaggag
     2881 gtctccctcc tccagcgcga acgcggtgcc gacggccgtc gcctcctgcc cgaggcaccg
     2941 gtagagcccg ccggtcatct tcccctggcg gtagaggctc accatccgct cctcgaggag
     3001 gcgggtgaga aggagatgcc tgtagaggga tctctgctgt tccagggaaa acgtcatcgc
     3061 gtcaggcggg cgcccaactc tcgattatcg ccaccgtttt cgcgatgcgt caaactggtg
     3121 tcgcatgacc cgccgcccgc cgaagacctc ctccctgggc cggctcctcg agctgggcgg
     3181 gctggccgtt cgtgcgggga cgtcgacggc ggcgtcgttc gtttcgagcc tcggacggcc
     3241 ggcggcgcgg agggaggagc ttcggatcga ggagctcgtg cggaacgcgc ggcggctcgt
     3301 cgcgacgctc ggggagatga agggcgccgc gatgaaggtg gggcagatgc tcagcctgca
     3361 ggacgccatc ctcccgccgg aggtcgcggc cgtcctgcgg gcgctgcaga aggagtcgcc
     3421 gagcctccct ctcgagatgg tcgaggagca gctcgcggaa gagctgggcg acccgctcca
     3481 gctcttcgtg agcttcgagc cggaggcgtt cgcggcggcc tcgatcggcc aggtgcaccg
     3541 ggcggtcctc cgcgacggcc ggcgggtcgc ggtgaagatc cagtatcccg ggatcgaccg
     3601 gatggtcgaa gccgacctcg ggaacctgcg gcgggtcctg aagtcggtcg tcgcgctcgt
     3661 ctcgaaggtc gacttcgagc cgatctggca ggagctgcgg gcgcgcctgc gcgaggagct
     3721 cgactacctc cacgaggcgg agcggatgcg gcggatggcc gagctccatg ccgacgtccc
     3781 ggaggtcgtc atcccgaggg tcgtcgagga ggcctccacc gggaggatcc tgacgaccga
     3841 gtacgaggag gggctctcgc ccgacgaggc gtgcgcgccg gagactccgc aggagctgaa
     3901 ggaccgctgg ggcgccgtcc tcttcgactt cctcctccgc ggcctcctcc gccaccgcct
     3961 cctccacgcc gacccgaacc tcgcgaactt ctcgttccgg ccggacggcc gcgtcgtcgt
     4021 ctacgacttc ggctgcgtga aggaggtgcc gctgcgcctc gcccgcggct accgcggcct
     4081 ctgccgcgcg gcgctcgagg ggcggacgga cgacctcccg gacgtcctga agcggatggg
     4141 gatgcaccgc ctcgacggct ccccgctcga ggagcggctg atcacgccgt gggccgccct
     4201 cgtcctcgag ctcctccggc cggcgccgcc ctacaggttc ggctcggacg actccgtcgc
     4261 gcgccgcctg ctcgacgccg cccgcacgag cctggcggag gcgggcgacg tccgatttcc
     4321 gcgggacatc gtcttcgtca accggacggt cctcgggcac ttcggcaacc tcgcgcgcct
     4381 gcgcgccgag gggccgtggc gggatatcct cgggcggttc acggagaagg agtgaccgaa
     4441 ccgggcgccg gcacggcgtc cggagaggct tcgccccgag gaggccgcga gatgtcgaat
     4501 acccccggca gcagctacga cgaggtcccg tacgtcgaca cgtgtcacgc cgcgacgcac
     4561 ccggaccgcc tcgccacgct cgccgcggtg tacggcctcc cggcgccctc cctcgagcgc
     4621 tgccgggtcc tcgagctcgg ctgcgcccgg ggcgggaacc tccttccgat ggcgatggag
     4681 ctgccggagg cgtctttcgt cggggtcgac ctctcggcgc ggcaggtcgc cgaggcgcgc
     4741 gagcgcgccg aagcgctcgg ccttcggaac gtcgctttcc atgcgatggg cctcgaggag
     4801 atcgacgccg gcttcgggac gttcgacttc gtcgtctgtc acggggtcta ctcgtgggtc
     4861 ccggagccgg tccgggagcg gatcctcgag atctgcgcga cgaacctctc cgcggagggg
     4921 ctcgcctacg tctcatacaa cacgtatccc ggctggagct cgcgcggcat ggtccgcgac
     4981 ctcgtccgct tccacgcgcg ctcggaagag acgccggagg ggcgcgtgga gagggcgcgc
     5041 ggattcctgg aggagctcgc ggggcacgtc gtccggcagg aaggggcata cgcgagcgtc
     5101 ctgaaagtgg aagcctcgcg tttccgcgag gccgacgaca cctacctcct ccacgagttc
     5161 ttcgaggacg acaacgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttcc
     5281 gcgaggccga cgacacctac ctcctccacg agttcttcga ggacgacaac gccccctgct
     5341 tcgtccacga gttcctgcgg cgcgcggagg ccgcgggcct cgagttcgtg gccgaggctc
     5401 aggctcccgg cctcttcgac ggcctgccgg agtcggcgcg tgagctcgtc tctcgctggg
     5461 ccggcggcgt cgcggcccgg gagcagtacc tcgacctgat ggtcaaccgg acgttccgcc
     5521 ggacggtcct tcgtcgggcc ggcgggccgc ccgccgtcgc gccggcaccc gaggtcgtgg
     5581 agcgcctcgc ggcgcgcgcg gtcgtcgtcc cctcgccggt cgcgtgggat gcggaaggga
     5641 aggacgggcc gatcgagttc gtccaccccg gccggagcgg ctcgatccag acgaacgacc
     5701 cggcgctgaa ggccgccctc ctcgctctcc acgaggcgag gccggcggcg cttccgttcg
     5761 agacgttgtg gaagcgggtc cgcgaaggac tggccgtccg cggagcgcct tcgccgcgcc
     5821 ccgcacgtga gacgcgcgag gcccttcgtt cgtcgctcct ccgtctcttc ctcgccgact
     5881 tcgtcgagct ccacgtccgc ccgccccggc ccgcgaccga cccggcggag cggcccgtcg
     5941 gcagcccgtt cgcccgccgc caggccgcgg acggcaaccg cgtcacctcc ctcgcccgca
     6001 ggcgcgtcga gctcgacgag ctcgcccggg ccgtcctcct cgagctcgac ggggcgcaca
     6061 ccgaggcgga ggtcctggac gcggtcgtgg cccgggtctg ctcgggatcg ctcgagctga
     6121 gcgacgagtc ggggccggtc cgggaggcgg ccgccgcgag ggcgctcctc gcgggtgaga
     6181 tccgtccggc gatcgagagg ctcgcgaggc tggcgctcct ggccggctga agcggccctc
     6241 gaatcgacac cggggcaggg ccgcgccgcc ggggcgcaac atcccgccgc cgaccttgcc
     6301 cggccgggcc ggacctccct atgctcgaag gatgatcgcc gtcttcgtcc tctacatggt
     6361 cgcgacggtc tcgctcgcct tctggtactc gcgcggggcg cgctcccacc gcgacttcgt
     6421 cctcggcggc ggccggttcg gcggcacggc gctcgcgctc tcggagcggg cgacgggcga
     6481 gtcggcctgg ctcctgctgg ggctgacggg gcacgcctac gccgacggcc tctcgacgat
     6541 ctgggtcgcc ctcggctgcg tcctcgggat cctcttcatc tggatggtga tggcgcggcg
     6601 tctccgggag gagacggaga ggaccggggc gatgacggtc tcgagcctcc tcgcgcgccg
     6661 cttcccgggc tcggagaagc cgatcgccct cctctcggcg ggcatcgtca tcttcttttt
     6721 catgctctac atcgccgcgc agttcagcgg ctcggggaag gtcctccagg agacgttcgg
     6781 cctcgacccg ctctggggga tcctcatcgc ctcggtcgtc gtcacgatct actgcggcct
     6841 cggcggcttc atcgccgtcg tcgtgacgga cgtcttccag gcgctcctga tgatcttcac
     6901 gctcgtcgcc ttccccgtca tcgcgctcgt gctcgcgggg agcaagggga tcgacctcgc
     6961 ggcggccttc gcggcggcgg ggccgagcta cctttcgctc acgggcggga agacgggggc
     7021 cgcggccgtg atcctcgtcc tctcggggct cagctgggcg ctcggctaca cgggccagcc
     7081 gcagctcctg acgcggatga tggcgatccg gagcgagaag gacgtgaaga cggcggtgag
     7141 ggtcgcatcg gtgtggacgc tcttcgccta cgcgggggcg atcctgatcg ggatgatcgg
     7201 cgtcgccttc gtccagtcgg gcgcggtcgg gggcggcgtc ggaaagctcg ccgacgccga
     7261 gaaggtcctc cccgcgatgg tgacgacgct cgtcacgccg atcctcgcgg gggtgctcct
     7321 gtccggcgtc atctccgcga tgatgtcgac cgcctcctcg gaggtgacgg tctcgtccgc
     7381 ttcgttcagc gaggacgtcg tcgcgatgct ccggaagaag gcggcgacgc cccgcgggat
     7441 gctcctcctg aaccagggag cgaccgtcgc ggtgggtgcg atcgccatcg ttctcgcgct
     7501 gacgatgcgc gacaccgtct acagcctcgt ctcctacgcc tggtcgggga tcgggtcttc
     7561 gttcgggccg gcgctcatgc tcctcctctt ctggaagagg ctgtcgcggg cgggcgtctt
     7621 cgcctcgctc gtcacgggga cggtcgcgac ggtcgtctgg aagagctttc tcgagcagcc
     7681 gacgggcgtc tccgagcgcc tcgcgagcta cgtcgtcgcg ctggcgatgg cggtggcctt
     7741 ctccttggcg ttccccgaga agcgccggac gtgaccggga acgcccgaga gaggaacgac
     7801 gatggcgaac gacacgggcc ggagctacga cgaggtcccc tacggcgagg gctgcttccg
     7861 cgtcagccag cccgaccacc tcgcggccct cgcggccgtc caccggatcc cggcgccccc
     7921 gctcgagcgg tgccgggtcc tcgagctggg ctgcggcagc ggcgggaacc tgctgccgat
     7981 ggccctcgag ctcccggagg cgaccttcgt cggcgtggac ctgtcggaag ggcagatcgc
     8041 ctcggcccgc gagcgcgcct cggcgctcgg cgtccgcaac gccgacttcc gggcgatgga
     8101 cctcgcggcg atcgacgcct cgttcgggac gttcgactac gtcgtctgcc acggcgtcta
     8161 ctcgtgggtg cccgaggccg tgcaggagcg gatcctcgcg atctgctcgg cgaacctctc
     8221 cccggacggg ctcgcctacg tcagctacaa cacgttcccc gggtggcacg agaggcggat
     8281 ggcgcgggag ctgatgctct accacgaccg ggccggcggg ccgtcggcgg agaggatcgc
     8341 cagggcccgg agcttccccg gggagctcgc gcgcgtcgtc ctgagccccg agagcgccta
     8401 cggccgcgtc ctccgggccg aggcggaggc cttgaaggac gaggaggacg cctacgtctt
     8461 ccacgagttc ctcgaggacg tgaaccggcc gacgtacgtc cacgagctcc tgacgcgcgc
     8521 cgcgcggcac gggctcgagt tcgtgacgga ggcggggacg cccgggctct tcgcggggct
     8581 cccggcgccg tcgcgcgagg ccgtcgagcg ctgggccgtc gacgtgatct cgcgggagca
     8641 gtacctcgac ttcgtctgca accgcccttt ccgcaagacc gtcctccgtc acgccggcgg
     8701 gccccccgcg ggggcgcccg acccggccgc cctcgccggg ctcgaggtga gcagcgacct
     8761 gaagccggcg acgggcagcc ccgtcgtcgc agacgagtct cccgcacgct tcacgcggcc
     8821 ggacgtcgag gggggggcga gcacgaccag ccccctcctc aaggcgatcc tcctcgcgct
     8881 cgacgcggcg cgcccgcggg cgctcgaccc ggcggcgctc cacgccggcg tcgccgcccg
     8941 gctcgccgag gccgggagag cgcttccggc cgaccgggaa gaggcggagg cccagctgcg
     9001 cggcgccgtc ctccttctct tcctttccga cctcgtcgag ctgcacgtgc gtgcgccccg
     9061 cccggcggcg ggggtctccg cccgacccct cgccagcccg ctcgcacggc accaggcgtc
     9121 gcggtcgacc cgcgtgacga gcctccggcg ccgcgtcgtg gaggtgggcg agctcgagcg
     9181 ggccgtcctc ctcgagctgg acgggcgccg cgacctcgac ggggtgctcg accgggtcac
     9241 ggcgcgggtc ctcgcgggcg aggtcgagct gagcctcgag gggaagaccg tgcacgaccc
     9301 ggccgtcgtt cgggccgcgc tcgcggtgga gatcgagccc gccgtgcgcg ccctcgtgga
     9361 gaaggcgctc ctcgcggggt agggcgtcgc gccccgccct cgcttgacag ccggccgccc
     9421 ggtccatagc ctgcgcggcg catgaacccc gcgactccgc gaaagcggac gacgaccacg
     9481 atgaagcgca cgccgcgagg cgggctggcg gggggccggg cgatcgtgta gcacgcggtc
     9541 gaacggaacc ggagaagaag ccagggcccg ccggaaacgg cgggcccttc gatttgcggc
     9601 ggagccgcgg aagaggacga catgacgaat ccgaagcagg acgggaggat cccggtggcg
     9661 gtcctcgggg cgaccggggc ggtggggcag cggatggtct cgctcctcgc gggccacccg
     9721 acgctgaagc tcgccgaggt ggccgcctcg gagcgctcgc aggggaagag ctacctcgag
     9781 gcgacgcgct ggatcctccc cggggacgtg ccggaggagg cccgcggcct cgtcgtgaag
     9841 ggcgtcgggg ggccgctcga gtcgcggctc gtcctctcgg ctctcgacgc cgccgtggcc
     9901 gacaccgtcg agccgctcca ggcctcgcgc ggcaagctcg tcgtctcgaa cacgaagtcg
     9961 ttccggatgc aggccgacgt gccgctcctg a
//
LOCUS       DAOTEZ010000121         9988 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4767_length_9988_cov_5.369778, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000121 DAOTEZ010000000
VERSION     DAOTEZ010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9988
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4767_length_9988_cov_5.369778"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="P5164_08040"
     CDS             complement(<1..206)
                     /locus_tag="P5164_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43881.1"
                     /translation="MSDEPSETPVLDWSAVTSVGNEAEATFIVGFLESRGIPARIVDK
                     SFSQNPVPNDEDLASIEVAVPVGRL"
     gene            complement(362..646)
                     /locus_tag="P5164_08045"
     CDS             complement(362..646)
                     /locus_tag="P5164_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43882.1"
                     /translation="MSEPTIRPPAPHGASPDGPVGGAEAPFAFDPGVDWQNAALDVVR
                     KHPVPCLLGAAAIGFLIGRHRGKAITAAVAGLATNAVMKQLSRAFDATEF"
     gene            complement(646..924)
                     /locus_tag="P5164_08050"
     CDS             complement(646..924)
                     /locus_tag="P5164_08050"
                     /inference="COORDINATES: protein motif:HMM:NF039524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43883.1"
                     /translation="MAETIDAAKAVDEKIDAARESFGGRVKEKVGEVAEEVKSRAGVL
                     RDRLRETDWDEVTEKASDWVRQNPGKSVAIALGVGFAIGLLFRRRSDD"
     gene            1027..2415
                     /locus_tag="P5164_08055"
     CDS             1027..2415
                     /locus_tag="P5164_08055"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY43884.1"
                     /translation="MQIGFHGAARTVTGSKYLLTLDRYRLLVDCGLFQGGFEMHQRNW
                     RGLPFSASSVDDVLFTHAHIDHTGYFPRLAADGFSGRAFATPPTKALLGVLLPDSGGL
                     QEEEARWANKKGYSRHQPARPLYTEEDANRSLSLLNKLEFERETPLHKRLKVTLHRAG
                     HILGAAFAEIRYRDDGSDWKTVVFSGDMGRRGVPILKDPSPLPACDVLVCESTYGDRL
                     HEASDPKEQLRRELEEAFRRGGVTVIPAFAVGRTQEVLYHLFELFEEGRLPKVPVFVD
                     SPMANSVVELYCRYESEHDVEMNALEEAAGSPLQTPYFTVVRKREDSKRLNDHPGPAV
                     VVSASGMATGGRVLHHLYHRLPDARNTVLFVGYQAEGTLGRRLLEGETEVVVMGDGLT
                     VKAAIRQIPALSAHADADELIAWMKTAPAPPKTLFLTHGEPAVQDAFAARIRSELGWN
                     VHVPEPDEVAEV"
     gene            2526..3818
                     /locus_tag="P5164_08060"
     CDS             2526..3818
                     /locus_tag="P5164_08060"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HRY43885.1"
                     /translation="MAARGTSLAAGLAYSSLLSFIPLVASVTVLTSTFFGEEGGKGFY
                     RLLRFFVPGTSREVLRGVAALVDVARAASGIATALLALTSLKVYFDVEGAANHLWGTL
                     RPRRVRERLAFAALTVVLGPVALGVATSLLLERGFPITRLHAGGVLASIVGLALIYKV
                     VPSAQVRWGAAAAAAILVGSGLIVLRQVFTAGFIALAGIERLYGTISAIAIFVIATGF
                     AWTILLFGFSFAHAVQFRDELLAHDAPPPAAKKPGPLEETTQLLLRLTEAWHAGVSLD
                     ASTLAETCSRGRPDDLRSRMKRLDTAGLVVVEGDAIRLARPPDTITLYAVARAVGDAA
                     PRAVPKGADPASAILRALYMRADAEERGVLQGTSLEDVYRPHARRRSAETDHGNLRLG
                     GEATAPGRKGGSGGKHGSGVVPRAGGHAPPAEPEDGAV"
     gene            3859..3934
                     /locus_tag="P5164_08065"
     tRNA            3859..3934
                     /locus_tag="P5164_08065"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3892..3894,aa:Ala,seq:cgc)
     gene            4316..4645
                     /locus_tag="P5164_08070"
     CDS             4316..4645
                     /locus_tag="P5164_08070"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRY43886.1"
                     /translation="MTELIEVHQAALAELCRRFGVDRLYVFGSAASGDLRPSSDVDLV
                     VSMADRLPTGTYADRFLDFAEAVEGLLGRRVDLVSEQAIRNPFFRREIEATRQLVYGQ
                     PLEETAV"
     gene            4614..4967
                     /locus_tag="P5164_08075"
     CDS             4614..4967
                     /locus_tag="P5164_08075"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRY43887.1"
                     /translation="MDNRSKKLLFDVVSAGRSITEWCGGRTYLEYEDDRQLRRAVERE
                     FEVIGEALNRLEQGDPETAARIEDLRRIVGFRNRIIHGYDTIDDATVWGVIEGRLPRL
                     IEQVSDLLVQAGEHD"
     gene            complement(5338..7407)
                     /locus_tag="P5164_08080"
     CDS             complement(5338..7407)
                     /locus_tag="P5164_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRY43888.1"
                     /translation="MKPPLLPLLLAAASAALVPFAVRAEEPPAGWEAYALARVTVTAE
                     AAPVDETALTVAVTAEEIAARNAKNVAEALAAVPGLRVSAGRKNEPTVYLHGFDQSRV
                     QVLIDGVPYYEANYGRLDLAQIPTDNVARIEVTKGAASVLGGANALGGTINIISRTAG
                     AKPFTELLVEGGDYATGRFVATHGARRGPLSYWLSLSGQTSGGTPVPGGFSPKTGTIQ
                     QKNPTKNTPAVIEDGGRRENSDTKQRNAWAKLGWEPSKATALFLNVRYFDRDKGAPPA
                     TDAVQVFLKRPAFSQFSRIPTYRDLAVDLDGRHELAQGLTLKAKAFHHEHVDDYESYK
                     DETYSERIAISRFDDTLTGGSLVAEWAAASWSTLRLAAHLKDDTHRERDDAYLPFAES
                     ASITGSLGLEDSFTLSGRLSAVAGVSYDWFDVSRAERNVTDGSTGDFVRQDRLATPND
                     EQLNPMAGLVWRSASGTVVNASLARKSRFPTLSQLYSTKSGNTGLASETSVNATAGVS
                     RPLGANVRVEAGVFLYDVRDLITRNGSSATNLYRNVGKVRMAGLETVLEVTPLSGLTL
                     RADYTFVDAKERTTARVTDDVTNVPRHKAGFLASWAAAAGTRVDLDAAWLGSMYSSLP
                     SVAYPKDPVKESAGYVLLGGKVTREVLDGLRLWVSGRNLLDEDYESEVGFPGVGRSFS
                     FGASATF"
     gene            complement(7699..8115)
                     /locus_tag="P5164_08085"
     CDS             complement(7699..8115)
                     /locus_tag="P5164_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43889.1"
                     /translation="MRARALGLALALAVSNLAASTADAKPPKEKGTKNARSAPGGQVR
                     ENGTPPGLARKGGLPPGLAKKVGPTRSEKVYVAFDRRYDDRAWFLIDGEWILKSGFEP
                     SVRAEVTASLALPPLAAPPPVPLPRIGVDLHVVLFP"
     gene            8352..9167
                     /locus_tag="P5164_08090"
     CDS             8352..9167
                     /locus_tag="P5164_08090"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRY43890.1"
                     /translation="MTTERGDYVLGTHGEEVRRLGIQHRVWRRCARAAWRRAGLRPGW
                     RVVDVGAGPGWATWDLAEAVGPHGAVLAVERAGQFLPVLEAERRRRGLEQVTTRDADL
                     MESAPPGGHDMAWCRWVASFVTSVPTLVRWVHGALRPGGTAVFHEYADYGSWRFAPPR
                     PRLAEFVAAVMESWRAAGGEPDVAPALVGALAREGFRLRSVRPLVFAARPGGRTWQWP
                     ASFVETNAERLLGLGRVSPDWAAEVVAELKAAEADPASVMVTPLVLEVIAERL"
     gene            9361..>9988
                     /locus_tag="P5164_08095"
     CDS             9361..>9988
                     /locus_tag="P5164_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020481114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43891.1"
                     /translation="MRLKLIVGFGVVLALLLLALLPGAAVSPTGWTVVAWNNLGMHCM
                     DADFALFAILPPYNTIHAQVVDPSGRLVKSGSSVSVTYEAVADPEGSINTTSRGKTNF
                     WDHVGSLFGAAVPVDAGLAGKDMPGPANAPKAMTFQAAQSWFVAEGIPITPYDDAGLK
                     NPYPLMKVTARDAAGNVLASTRIVLPVSDEMDCRGCHGSGTVEAARPAA"
BASE COUNT         1328 a         3555 c         3564 g         1541 t
ORIGIN      
        1 agccgtccca ccggcacggc gacctcgatg gaggcgaggt cctcgtcgtt cggaaccggg
       61 ttctgggaga agctcttgtc gacgatccgt gccgggatcc cgcggctctc gaggaacccg
      121 acgatgaagg tggcttcggc ctcgttcccg acggacgtga ccgcgctcca gtccagaacg
      181 ggggtttcgc tgggctcgtc gctcatggct cacctcgctt cgggattccg gccggccgga
      241 cgcctccgga gccgttcgac cgccgcaacg ccagtatggg ccagatcgcg acggaagagg
      301 cgccgaacgg cctgcgtgaa gggcgggcgc ggcccgttcg gcgggccgcg ccgcgcccgg
      361 ctcagaactc cgtcgcgtcg aacgcgcgcg agagctgctt catcaccgcg ttcgtggcga
      421 gcccggccac cgccgcggtg atcgccttcc cgcggtgacg cccgatcagg aagccgatcg
      481 ccgccgcacc gaggaggcag gggaccggat gcttccgcac gacgtcgagc gcggcgttct
      541 gccagtcgac ccccgggtcg aacgcgaagg gcgcctcggc gccgccgacc ggtccgtccg
      601 gggaggcgcc gtgcggggcc ggcgggcgga tcgtcggctc cgacatcagt cgtcgctccg
      661 ccggcggaag aggaggccga tcgcgaagcc gacgccgagc gcgatcgcga ccgacttgcc
      721 ggggttctgc cggacccagt cggaggcctt ctcggtcacc tcgtcccagt cggtctcgcg
      781 gaggcggtcc cggaggaccc ccgccctcga cttcacctct tcggcgacct cgccgacctt
      841 ctccttcacg cgcccgccga agctctcgcg ggcggcgtcg atcttttcgt cgaccgcttt
      901 cgcggcgtcg atggtctcgg ccatgtcgac ctcccttccg atacgggatg gaatcttcgc
      961 acgcccctgc ggcgcctcgc cagcgccgac cgccctccgg ggctaccatt cgagaaggag
     1021 gacccgatgc agatcggatt tcacggcgcg gcgcggacgg tgaccggctc caagtacctc
     1081 ctgaccctcg atcgctaccg cctcctcgtc gactgcggcc tcttccaggg cggcttcgag
     1141 atgcaccaga ggaactggag ggggctcccc ttcagcgctt cctcggtcga cgacgtcctg
     1201 ttcacccacg cccacatcga ccacaccggc tacttcccgc gcctcgcggc ggacggcttc
     1261 tcggggcggg ctttcgccac gcctcccacg aaggccctcc tcggcgtcct cctccccgac
     1321 tccggcggcc tgcaggagga agaggcccgc tgggccaaca agaagggcta ctcgcgccac
     1381 cagccggccc ggcccctcta caccgaggag gacgcgaacc gctccctctc gctcctcaac
     1441 aagctcgagt tcgagcgcga gaccccgctc cacaagcgcc tcaaggtgac gctccaccgg
     1501 gccgggcaca tcctcggcgc ggccttcgcg gagatccgct accgggacga cggctccgac
     1561 tggaagacgg tcgtcttctc cggagacatg gggcgccgcg gcgtgccgat cctgaaggac
     1621 ccgtcgccgc tcccggcctg cgacgtgctc gtctgcgagt cgacctacgg cgaccgcctc
     1681 cacgaggcgt cggacccgaa ggagcagctc cgccgcgagc tcgaggaggc gttccgccgc
     1741 ggcggcgtga ccgtgatccc ggccttcgcc gtcgggcgga cccaggaggt cctctaccat
     1801 ctcttcgagc tcttcgagga agggcgcctc ccgaaggtcc ccgtcttcgt cgactccccg
     1861 atggcgaact ccgtcgtcga gctctactgc cggtacgaga gcgagcacga cgtcgagatg
     1921 aacgccctcg aggaggccgc cgggtctccg ctgcagaccc cctacttcac cgtcgtgcgc
     1981 aagcgcgagg actccaagcg gctgaacgac catcccgggc cggccgtcgt cgtctcggcc
     2041 tcgggaatgg ccaccggcgg tcgcgtcctc caccacctct accaccgcct ccccgacgcg
     2101 cggaacaccg tcctcttcgt cggctaccag gccgaaggga ccctcgggcg gcgcctcctc
     2161 gaaggggaga ccgaggtcgt cgtcatgggc gacggcctga ccgtgaaggc ggcgatccgc
     2221 cagatcccgg ccctctccgc tcacgccgac gccgacgagc tgatcgcctg gatgaagacc
     2281 gcccccgcgc ctccgaagac cctcttcctg acgcacggcg agccggccgt tcaggacgcc
     2341 ttcgccgccc gcatacgctc cgagctcggc tggaacgtcc acgttcccga gccggacgag
     2401 gtcgcggagg tctgagactg ggccgcggca cgggagcctt tccggtccgg cccccggagc
     2461 ttcgggggat cacgagggcg gccgcccttg cctgggtcgt ccttcgccgg agcctccggt
     2521 cgtacctggc ggcgcggggg acctccctcg ccgccggcct cgcctactcc tcgctcctct
     2581 cgttcatccc gctcgtcgcc tcggtcaccg tcctcacctc caccttcttc ggagaggaag
     2641 gagggaaggg cttctatcgc ctcctccgct tcttcgtccc cggcacctcg cgggaggtcc
     2701 tccggggcgt cgcggccctc gtcgacgtcg cccgggcggc ctccgggatc gcgacggccc
     2761 tcctcgccct cacctcgctc aaggtctatt tcgacgtcga gggggccgcg aaccacctct
     2821 gggggactct ccgcccgcgg cgcgtccgcg agcggctcgc cttcgccgcg ctgaccgtcg
     2881 tcctcgggcc ggtggctctc ggcgtcgcga cctcgctcct cctcgagagg ggcttcccga
     2941 tcacgcggct ccacgccggc ggggtcctcg cgtcgatcgt cggcctcgcc ctcatctaca
     3001 aggtcgtccc ctcggcccag gtccgctggg gcgccgccgc ggctgccgcg atcctcgtcg
     3061 gctccggtct gatcgtcctc cggcaggtct tcaccgccgg gttcatcgcc ctcgcgggga
     3121 tcgagcgcct ctacgggacg atcagcgcga tcgcgatttt cgtcatcgcc accggcttcg
     3181 cctggacgat cctcctcttc ggcttctcgt tcgcccacgc cgtccagttc cgcgacgagc
     3241 tcctggcgca cgacgctccg cccccggccg cgaagaagcc cgggccgctc gaggagacga
     3301 cccagctcct gctccgcctc accgaggcgt ggcacgcggg cgtctcgctc gacgcctcga
     3361 cgctggccga gacgtgcagc cgcggtcgtc ccgacgacct gcggtcgcgc atgaagcggc
     3421 tcgacaccgc cggcctcgtc gtcgtcgagg gcgacgccat ccgcctcgcc cgtccgcccg
     3481 acacgatcac cctctacgcc gtcgcacggg ccgtcgggga cgcggccccc cgcgccgtcc
     3541 cgaagggcgc ggaccccgcg tcggcgatcc tccgcgccct ctacatgcgg gcggacgcgg
     3601 aggagcgagg cgtgctccag gggacgagcc tcgaggacgt ctatcgacct catgcccgga
     3661 ggcgttccgc cgagaccgac cacggcaacc tccggctcgg cggcgaggcg acggcgcccg
     3721 gacggaaggg gggctccggc ggcaagcacg gcagcggcgt cgtcccgcgc gcgggcggcc
     3781 acgcaccccc ggcggagccg gaggacggag ctgtttgacg aatcgccccg atccggctag
     3841 attctccgcc cccaaggtgg ggctgtagct cagctgggag agcgctagaa tcgcactcta
     3901 gaggtcggga gttcgatcct cctcagctcc accattcatt tcgaaagact tacgggccgc
     3961 ggcgacgcgg ccctttttct ctttgtccac acttctgtcc acgacagtct ggtggtcggg
     4021 agtttggggc cttcgacacg tcgttggtcg caatcgactt gccggccgtc gccatccgcg
     4081 aactcggctg gttcttcaac gggtctgtcc acattcgcca gaccgatcgg gagcctggcg
     4141 tcgtcgccga ggccctcggc acgcggcgga tccgcccgac accgtgttcc tgctccaggt
     4201 cgacatgggc caccggaggg tcccggaacg actccgctgg tcgccgagat cggtccgcga
     4261 tcgtgggggc gacagcgccc gacccgtgcc tgatacgatt tccgcagagg atgacgtgac
     4321 ggagctgatc gaggtgcacc aggcggcgct cgccgaactt tgtcggcggt tcggagtcga
     4381 tcggctctac gtgttcgggt cggcggcctc cggagacctc cggccctcca gcgacgtcga
     4441 cctcgtggtc tccatggcgg accgcctgcc gaccggcacg tacgcagacc ggttcctgga
     4501 cttcgcagag gccgtcgaag gcctgctggg gcgccgggtg gacctggtct ccgagcaggc
     4561 gatccggaat cccttcttcc gtcgcgaaat tgaggcgacc cgtcagctgg tgtatggaca
     4621 accgctcgaa gaaactgctg tttgacgtcg tgtccgcggg ccggagcatc accgagtggt
     4681 gcggcggtcg tacgtacctg gagtacgagg acgatcggca gcttcggagg gccgtcgaga
     4741 gggagttcga ggtgatcggc gaggcgctca accgtctgga gcagggcgat ccggagacgg
     4801 cagccaggat cgaagatctc cgccggatcg tgggcttccg gaaccggatc atccacggct
     4861 acgacacgat cgacgacgcg acggtatggg gcgtgatcga gggccgtctc cccaggctca
     4921 tcgagcaggt gagcgatctg ctcgtacagg ccggcgagca cgactgaggg ctgtcgcggc
     4981 cgggatcgcg tggaggccgt acagctgtcc acgcacgtgt ccacaatcgc cggacccatc
     5041 gggaacctcg tgactctctc ggtaccccgg gaatgtcgac gcggcacggc ttaggttgct
     5101 gccaatgaac gagttccgct gtccagagaa ccttgcggcg ccctcgggcg cccggcggct
     5161 cccgggtgcg aaagcacctc gacccggcat cgcactctag aggtcgggag ttcgatcctc
     5221 ctcagctcca ccattcattc caaagaattt agcgcccctc gcggggcgct tttcgtttcg
     5281 ggctggcccg ttcctcaccc cggcagggga ggcggcgggc ccgcgcccgc cctgtcttca
     5341 gaaggtggcc gaggccccga aggagaagct cctcccgacg ccggggaagc cgacctcgct
     5401 ctcgtagtcc tcgtcgagga ggttgcggcc gctgacccag aggcggaggc cgtcgagaac
     5461 ctcccgcgtg acctttccgc cgaggaggac gtagccggcg ctctccttga ccgggtcttt
     5521 cgggtaggcg acggagggga ggctcgagta catcgagccg agccaggcgg cgtcgaggtc
     5581 gacgcgcgtc ccggcggcgg ccgcccagga ggcgaggaag cccgccttgt gccgggggac
     5641 gttcgtgacg tcgtcggtga cgcgggccgt ggtccgctcc ttcgcatcga cgaacgtgta
     5701 gtcggcccgg agggtgaggc cggagagcgg cgtcacctcc aggaccgtct ccaaccccgc
     5761 catcctcacc ttcccgacgt tccggtagag gttcgtggcg ctcgagccgt tccgcgtgat
     5821 caggtcgcgc acgtcgtaga ggaagacgcc ggcctcgacg cggacgttcg cgccgagcgg
     5881 gcgcgagacg cccgccgtgg cgttcacgct cgtctccgag gcgaggcccg tgttgccgct
     5941 cttcgtggag tagagctggg agagggtcgg gaagcggctc ttgcgggcga gggaggcgtt
     6001 gacgacggtg ccggaggcgc tccgccagac gagccccgcc atcgggttga gctgttcgtc
     6061 gttcggggtg gcgagccggt cctgccgcac gaagtcgccc gtcgagccgt cggtgacgtt
     6121 cctctcggcc cgcgagacgt cgaaccagtc gtacgagacg ccggcgacgg cgctgagcct
     6181 gcccgacagc gtgaaggagt cctcgaggcc gagggagccg gtgatcgagg ccgactcggc
     6241 gaacgggagg taggcgtcgt cgcgctcgcg gtgggtgtcg tccttcaggt gggccgcgag
     6301 gcggagggtg ctccaggagg ccgcggccca ctccgcgacg agcgagccgc cggtgagcgt
     6361 gtcgtcgaag cggctgatcg cgatccgctc ggagtaggtc tcgtccttgt acgactcgta
     6421 gtcgtcgacg tgctcgtggt ggaacgcctt cgccttgagc gtcagcccct gcgcgagctc
     6481 gtggcggccg tcgaggtcga cggcgaggtc gcggtaggtc gggattcggg agaactgcga
     6541 gaaggcgggg cgcttgagga agacctggac ggcgtccgtc gcgggcgggg ctcccttgtc
     6601 gcggtcgaaa tagcggacgt tgaggaagag cgccgtcgcc ttcgacggct cccagccgag
     6661 cttcgcccag gcgttccgct gcttcgtgtc ggagttctcg cggcggccgc cgtcctcgat
     6721 cacggccggg gtgttcttcg tcgggttctt ctgctggatc gttcccgtct tcggcgagaa
     6781 gccgccgggg acgggtgtcc cgccgctcgt ctgcccggag aggctcagcc agtagctcag
     6841 ggggccgcgc cgcgcgccgt gggtggcgac gaagcgcccg gtggcgtagt cgcccccctc
     6901 gacgaggagc tcggtgaacg gcttcgcccc ggcggtcctg ctgatgatgt tgatcgtccc
     6961 gccgagggcg ttcgcgcctc cgaggacgga ggcggccccc ttcgtcacct cgatccgagc
     7021 gacgttgtcg gtcgggatct gggcgaggtc gagcctgccg tagttcgcct cgtagtacgg
     7081 cacgccgtcg atgaggacct ggacgcggct ctggtcgaag ccgtgcaggt agacggtcgg
     7141 ctcgttcttc cgcccggccg agacgcgcag gcccggcacg gccgcgagcg cctcggcgac
     7201 gttcttcgcg ttccgggcgg cgatctcctc ggccgtcacg gcgaccgtga gggcggtctc
     7261 gtcgaccggc gccgcctccg cggtcacggt gacgcgggcg agggcgtagg cctcccaacc
     7321 ggccgggggc tcctcggccc ggacggcgaa aggtacgaga gcggcggagg ccgccgcgag
     7381 gaggagcggg agaagggggg gcttcacggg gcctccgatt cggtcgcggc gcgcggcggg
     7441 gcccgtctcg ccgagcgggg agcggaggtg ctggccctct accaggagat ggagaccgga
     7501 gcgcggcgtg cggcgcaggg cccctggcgg cgcctgaagc gcctgctctc ccgagccctc
     7561 ggggagcctc gacctccggg cgacgtcgtc caaaccgacc tattttatcg gatatatcgc
     7621 tcttccggag tcgccgacgc ctggccacgc cccacctctc ggccctcggg gccggagggg
     7681 atggccgccg cggcgggtct acgggaagag gacgacgtgc aggtcgacgc cgatcctcgg
     7741 gagcgggacc ggggggggag cggcgagcgg cgggagtgcg agggaggcgg tcacctcggc
     7801 ccggacggag ggctcgaaac ccgacttcag gatccattcg ccgtcgatga ggaaccacgc
     7861 ccggtcgtcg tatctcctgt cgaatgcgac gtagaccttc tcggaacgcg tcggcccgac
     7921 cttcttcgcg aggccgggcg ggagcccccc cttcctcgcg aggccgggcg gcgttccgtt
     7981 ctcacggacc tggccgccag gcgccgatct ggcgttcttc gtccccttct ccttcggggg
     8041 cttcgcgtcg gcggtggagg ccgccaggtt cgatacggcg agggcgagcg cgaggccaag
     8101 agcgcgagca cgcatcgggg tcctccgggg ccgggcggca gcccgggcgg cgcgaaacgg
     8161 caacttcggc atgtctcggt cgaacgcagc attctccgtg ccgtcgatca gtgcgacagc
     8221 cgaaacccgc cggacgaacg tctcggaacg gactagattc gcgtcgtgac cgtccgcggc
     8281 cgggatcgtc ggcgggcttc ccgctgcgcg ggtcccgagc cttcgccgcg cggacgggcc
     8341 gggaggctgc ggtgacgacg gagcggggcg actacgtact cggtacccac ggggaggagg
     8401 tccggcggct cgggatccag caccgggtct ggcgcaggtg cgcccgggcc gcctggcgga
     8461 gggccgggct tcggcccggc tggcgggtcg tcgacgtcgg cgccgggccg ggctgggcga
     8521 cctgggacct cgcggaggcg gtcgggccac acggcgcggt cctcgccgtc gaacgggccg
     8581 ggcagttcct tcccgtcctc gaggcggagc gtcgccgtcg cgggctggag caggtgacga
     8641 cgcgcgacgc cgacctgatg gagtccgcgc cgccgggtgg ccacgacatg gcctggtgcc
     8701 gctgggtcgc gtcgttcgtc acgtcggtcc cgacgctggt ccggtgggtc cacggggccc
     8761 tgcgccccgg cgggacggcc gtcttccacg agtacgccga ctacgggagc tggcggttcg
     8821 cgccgccgag gccccggctc gcggagttcg tcgcggcggt catggagagc tggagggcgg
     8881 cgggaggcga gccggatgtg gccccggcgc tcgtcggcgc gctcgcgcgc gaggggttcc
     8941 gtctccgctc ggtccggcct ctcgtcttcg ccgcgcggcc cggagggcgg acctggcagt
     9001 ggccggccag cttcgtcgag acgaacgcgg aacgacttct cgggctcggc cgggtctcgc
     9061 ccgactgggc cgccgaggtc gtcgcggagc tgaaggcggc ggaggccgac ccggcgagcg
     9121 tgatggtcac cccgctcgtc ctcgaggtga tcgccgagcg cctctgacgc gcgagccggg
     9181 atcgcctcga cacggtccgt gagcggcgcg accggttcgg agtccgttcg gtcgcgtcgt
     9241 gggattccgg gtttaccgtc acttcacgtc aatcgggacc aggcgaggcc gcattgcacc
     9301 ggcagcaggg tgtagcgggc cgacgtgtat ccgaggcccg gttccggcga ggaggacgac
     9361 atgcggctga agctgatcgt gggattcgga gtggtcctcg cgctcctcct cctcgcgctc
     9421 ctgcccggcg ccgcggtctc gcccacgggc tggacggtcg tcgcctggaa caacctcggg
     9481 atgcactgca tggacgcgga cttcgcgctc ttcgcgatcc tcccgcccta caacacgatc
     9541 cacgcccagg tcgtcgaccc gtcggggagg ctcgtgaaga gcggctcgtc ggtctcggtg
     9601 acgtacgagg ccgtcgccga tcccgagggc tcgatcaaca cgacctcgcg cggcaagacg
     9661 aacttctggg accacgtcgg ctcgctcttc ggggcggcgg tcccggtgga cgcggggctc
     9721 gcggggaagg acatgccggg gccggcgaac gcgccgaagg cgatgacgtt ccaggccgcg
     9781 cagagctggt tcgtcgccga ggggatcccg atcacgccgt acgacgacgc ggggctgaag
     9841 aacccctacc cgctcatgaa ggtgacggcc cgcgacgcgg cggggaacgt cctcgcctcg
     9901 acccggatcg tcctgccggt ctcggacgag atggactgcc gcgggtgcca cggctccggc
     9961 accgtggagg cggcgcgccc cgcggcgg
//
LOCUS       DAOTEZ010000122         9972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4777_length_9972_cov_6.163759, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000122 DAOTEZ010000000
VERSION     DAOTEZ010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9972
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4777_length_9972_cov_6.163759"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="P5164_08100"
     CDS             complement(<1..327)
                     /locus_tag="P5164_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43892.1"
                     /translation="MYVAYFDESGTDERSRSFCVAGFVGHQADWFELSRVWRALLRES
                     GLPAFHMTDFEARRGACAHLPESARVPLIDGLVTAIEGLQLMGIGAGVVRAHYDEVVV
                     ASGWLQR"
     gene            complement(693..1052)
                     /locus_tag="P5164_08105"
     CDS             complement(693..1052)
                     /locus_tag="P5164_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43893.1"
                     /translation="MARRSRFLTALLVLFGLHACASAQSVMNSWLGHDQSDLIRSWGP
                     PSSVASDGAGGTILIYIVAPSPAVTAPAQLALNDPNQPEWVKQTARANIQANRPMQRM
                     MYVRADGTIYYWRIAPL"
     gene            complement(1517..2071)
                     /locus_tag="P5164_08110"
     CDS             complement(1517..2071)
                     /locus_tag="P5164_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HRY43894.1"
                     /translation="MKIEALVQLDLFSEAPPTRAELVADQASLQALYDAFAEAYRLPP
                     ARVELSSRRATGGVIQYGPPHVIRVSAHMSPADRRETLLHETAHAICHDRWGVDEGHS
                     SRFWEIARELGVTRKAAPETDRLATIRARNARYVYRCIGCLDEWTRRSPFGRARLCAA
                     CEGKGRPSRLVLVRRPKRAARRKG"
     gene            complement(2068..2658)
                     /locus_tag="P5164_08115"
     CDS             complement(2068..2658)
                     /locus_tag="P5164_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43895.1"
                     /translation="MTRTAERTILPALAVVAFLAAAYAWLPLARALRYATVAAFTRPA
                     AWHLALLPALLAVALHELLVRGLLYGALRSRLRVGFAAPAVALVGAIVPVAARYLLFP
                     FPAVPTPLVLGQALFVEYPISLALCLLALGTGSWKSSAAALYLLWAARILVVPTFRGA
                     PVPILEVLAALLVPLVVALVLHRPLAPHREALEGLS"
     gene            2713..3618
                     /locus_tag="P5164_08120"
     CDS             2713..3618
                     /locus_tag="P5164_08120"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY43896.1"
                     /translation="MSSNASLCAGFVGLGTMGLGMARNLAAKGFPLSLTNRTTEKAIR
                     LASDLAGGLAPVAALSTPAAVAAASDVVVSCVSDGPDVEEVHLGRNGTIEGARPGAVV
                     VDCSTIDPTVARLVAARLAEKGISFLDAPVSGGQKGANEGTLSFFVGGEAEALGKARP
                     VLEAMGRRITHLGPSGAGQLGKATNQVIVAGTLAAVSEGMAFAKAAGLPMEAIHAALT
                     GGAANSWALEVLGKKMLDRDFKPAFAIKHQQKDLAIVLRAARANGVPLPAASLVHQLL
                     SSLEAKGRGEEGTQALYTLYEELSR"
     gene            complement(3655..5469)
                     /locus_tag="P5164_08125"
     CDS             complement(3655..5469)
                     /locus_tag="P5164_08125"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRY43897.1"
                     /translation="MCGALRRRGRPVAAGLLVPLLAALAGPLSAADAPDEAERVRLLE
                     QSIRPRSVVSSPLGMVVTSAPEATWAGVRMLEAGGNAVDAAVAAAFALTAADPGGSGL
                     GGQTWLVLRTASGEERAVFCPAPVPIRIDRRRLRAARDGADLWGPMAAAVPTTVATLA
                     HALRVYGTKPFAEVLAPAIEAAESGYRTQVYEHGYLGDYRRRLFDSDVLYPVYLTGPV
                     DDSGIPGPAPLGTCVRLPNLARTLARLAERGLEDFYTGEIAATLEREVLAAGGFLTRP
                     DLARIPGRVLDVPPVRGSYRGLTALSVPSPGGGGVLVMALQVLGALEEERLREPGLAR
                     GHAIVEAVRLARAEAAAHRGQPEVPEGPLRSEWLSPAWAERQARRIRADRALPPSELG
                     DALPFASGIRGTTHVSVADAHGNVVSLTQTLGRYYGAAWAAPSLGFLLNAFLEPLVSD
                     DPASAEYLKAGGVVPMPVSPLLLLRDDRPALIAGAPGSSRIPSILLSLVTAFVDGGDT
                     PAAAYARPRMVWEDDLAGPRVMLELAPPFTERDVAALEAMGYPSVWALRAPSRDSGVF
                     GGIYGVARDEARGVWTGIVDGRRAGVAAAPARVPPPAR"
     gene            5591..6061
                     /locus_tag="P5164_08130"
     CDS             5591..6061
                     /locus_tag="P5164_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43898.1"
                     /translation="MKRAALALATLFVLVALSPAAAADPGERKSYTKTFPFGAGKESK
                     VGIRQGPVTIESVRLRNWPDADDFADAENDAGETHTMVVEFEYSNRDEARDWKCRYTV
                     TVPGKDGGSWAENDRTATLDSGKVHDTNKVFVRMKTRNYKRATSYRVTFEIWKD"
     gene            complement(6194..6769)
                     /locus_tag="P5164_08135"
     CDS             complement(6194..6769)
                     /locus_tag="P5164_08135"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY43899.1"
                     /translation="MKIAFIGTHGVGKTTLCYDLAAALKRRDLTVELVREVARECPLP
                     INRDTTIKAQAWILHTQIAWELQAEAKAEVVVCDRAVLDNYCYLKRAAPGDPQVKVLE
                     ELVKAWLPTYDALFRVPIVGDPSFDGVRDTDLAFQHDIDALLLATLEDWGVPFVALDA
                     VCREGWAKCAIESLLPALRPQELLFPEAAGR"
     gene            complement(7023..9365)
                     /locus_tag="P5164_08140"
     CDS             complement(7023..9365)
                     /locus_tag="P5164_08140"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HRY43900.1"
                     /translation="MTEAAAPRPKPRVPPGALVGGFVVLLAGAVAAHLVLPEWQPLGV
                     SKEAAIATARAEMAAVGATLLAPKAGVRNGLASSELERAYRRLGREAPRWLARHGGLA
                     SWVVSGSLRVPGVGTGPAEVAIARDGTLRRVEFTSGSVFRVAAPSGEVQAARERFAAG
                     VLGRLARGEALGAPTDHESGSTIVRVWELGPSREGPREVVTRVAPTTAILVLSRELAD
                     PDIGARYEKLTFRGLAIAIPLFGVVGLGVLVVFGVLLFRRRLGFRIAIGLGLLGVAGM
                     VLSGALFELGGQRGLLAGFIVTGRLLVAVALVGYWAVAEAMLRDSVPGFRTSLDALVA
                     GRLTPRLGSAILGGLGLGGAIAGLRLLFASSVAAAGVPGVYAEAPSYVLPLFGGIANA
                     FLEGPYAAATLLLGAALARFVLPPERADVLGAVLFALFYSCSFLYAPWGALLVLTLAE
                     AAFLLLAFRSHGLTGLLVAATAPALFRDLLAASRFPGELFVPLLLTAASLAALACVGV
                     VASRRPEREDEARVDAPEFVKRLEAERRVKYEMDLLSRMQLALLPDAPPDVPGLQIAA
                     RTIVATEAGGDLYEFLVDEEGALWVAAGDVAGHGYSCGIQGAMVKACLLSLVKAGRRP
                     GEVLAEIDRVLRAGRAARLFTTLVLVRIEPATGRVVLANAGHPWPLLVVEGRVRELSV
                     SGLPLGKGPGRTYSELELELPGAASLVFASDGLFEGPDRFDDPYGYERPRGVLERIGL
                     WRRPADSILEALLADWRTHIGEGEPADDTTLVVVRRTIWS"
     gene            complement(9379..>9972)
                     /gene="nadA"
                     /locus_tag="P5164_08145"
     CDS             complement(9379..>9972)
                     /gene="nadA"
                     /locus_tag="P5164_08145"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF014498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRY43901.1"
                     /translation="LSDVIVTSSSAEAILNQIPDPVVFAPDKNLAAWLEKKLGRKFVV
                     WPGTCVVHETFSERRILDLMVRNPSARLVAHPECDERILAHAHFVGSTSALLRHVTES
                     EHRSFIVATEEGILHQMRRAAPGKELLAAPPDEGCSCNACPHMKRNTLEKLWLALRDL
                     SPAIEVPEETRLAAKRSLDRMLELTAGKTFVPALGRS"
BASE COUNT         1559 a         3334 c         3900 g         1179 t
ORIGIN      
        1 gcgttgaagc cagccactcg cgacgacgac ctcgtcgtaa tgcgcccgca cgacaccggc
       61 tccgatcccc atcagttgca gcccctcgat cgcagtcacg agcccgtcaa tgagcggcac
      121 gcgggcactc tcgggcaaat gggcgcaggc gccccgccgc gcctcaaaat ccgtcatgtg
      181 aaacgcggga aggccgcttt cccgaagaag tgcccgccaa acgcgagaaa gctcaaacca
      241 gtcggcttga tggcctacga agccagcgac acagaacgag cgggagcgct catcagttcc
      301 gctctcgtcg aaataggcaa cgtacatcgt atcggactgc gtgcggctgc acttgccggt
      361 gtcggtcgat cgatgacctc tgcggagcgt ctgcggcggc cgcaatctag gagccgcaac
      421 tgagccgcgc cgggaatcga tcgctacggc ctcttcttct tcggcttgaa tgggtcggct
      481 gtaccagact tgccggtgta agggttgaca tttcccttcg aagaccagtt gtccatacgt
      541 gtggtattcg gggccgttcg cttgtgtggc gatacgtaag tcccgttctt tctgatcgac
      601 ggcttcacgg acaccgactt ggtcgcgaag aggggatgag cgaagaggca gagaagcagt
      661 acggcggaaa cacggagaga catagccttc tcctacaagg gggcgatgcg ccagtagtag
      721 atcgtgccgt ccgcgcgaac atacatcatt cgttgcatcg gacggttagc ctggatgttt
      781 gccctggccg tctgcttgac ccactcgggt tggttgggat cgttcagagc gagttgggct
      841 ggagcggtga cggctggtga tggcgcgacg atatagatca ggattgtgcc gccggcgcca
      901 tccgaagcca ctgaagaggg aggaccccat gaacggatga ggtccgactg atcgtgcccg
      961 agccaggagt tcatcacaga ctgggccgag gcacaggcgt ggaggccgaa cagaacgaga
     1021 agtgcagtga ggaaacgcga gcggcgcgcc atgagttccc tcccgccgta gtctatagcc
     1081 gaaaccgaaa gctggagctg cctaaccctc tattagacag ctggccggcg tggtaagccg
     1141 gtcgaagagc tccgtcttag acatctcggt ccgctatcta ttccccgctc ctcacaagac
     1201 gaacgcagcg caagagcccc gattcgcgcc gtctaagccg ggcggaatgg acggcgtccg
     1261 gggggcaact ctctcccccg gcacggtggt ttcgttcggg cggcatcgag gccgcgggcc
     1321 tccgctcgac gacggagggg agacgccccg ggggctcgcc gcgccagccc gggacggcgt
     1381 aagggcgcgc tccgcgcccc tcggtggcag agaccgagcg cgggaagcgg gcggaccaag
     1441 ggcctcgggc acgagtcctg gagcgggaaa tccgagggag catcgcgcgg ccggacacga
     1501 cgcgcaaggg ctcgcgtcag cccttgcggc gggccgcccg cttcgggcgg cggacgagga
     1561 cgaggcgcga ggggcgcccc ttcccttcgc aggcggcgca gaggcgcgcg cggccgaagg
     1621 ggctccgccg cgtccactcg tcgaggcagc cgatgcagcg gtagacgtac cgggcgttcc
     1681 gcgcgcggat cgtcgcgagg cggtcggtct cgggagcggc cttccgcgtc acgccgagct
     1741 cgcgggcgat ctcccagaag cgcgaggagt gcccctcgtc gacgccccag cggtcgtggc
     1801 agatggcgtg cgcggtctcg tggaggagcg tctcgcggcg gtcggccgga gacatgtgcg
     1861 ccgagacgcg gatgacgtgg ggcgggccgt actggatgac gcctccggtg gcgcggcgcg
     1921 aggagagctc gacgcgcgcg ggcgggaggc ggtaggcctc ggcgaaggcg tcgtagaggg
     1981 cctggagcga ggcctggtcg gcgacgagct ccgctcgggt gggcggagcc tcggagaaga
     2041 ggtcgagctg gacgagcgct tcgatcttca cgacagtccc tccagcgcct cgcggtgagg
     2101 cgcgagcggc cggtggagga cgagggcgac gaccaggggg acgaggagcg cggcgaggac
     2161 ctcgaggatc gggacggggg cgccgcggaa ggtggggacg acgaggatgc gcgcggccca
     2221 gaggaggtag agggcggcgg cgctcgactt ccagctgccc gtgccgagcg cgaggaggca
     2281 gagcgcgagc gagatcgggt actcgacgaa gagggcctgg ccgaggacga gcggcgtcgg
     2341 gacggccggg aacgggaaga ggaggtaccg ggcggcgacg gggacgatcg cgccgacgag
     2401 ggcgacggcc ggcgcggcga agccgacgcg cagcctcgag cggagcgcgc cgtagaggag
     2461 gccgcggacg aggagctcgt ggagagcgac ggcgaggagc gccgggagga gcgcgaggtg
     2521 ccacgcggcg gggcgcgtga aggcggcgac cgtcgcgtag cggagggcgc gcgcgagggg
     2581 gagccaggcg tacgccgccg cgaggaacgc gacgacggcg agcgccggga ggatcgtccg
     2641 ctccgccgtc cgcgtcatgc gcgaatgtta gcggacgttg acgcctcccg acagggcgga
     2701 gagactcgcc ggatgagctc gaacgcatct ctctgcgccg gcttcgtcgg cctcggaacg
     2761 atgggcctcg ggatggcccg caacctcgcc gcgaagggct tccccctctc cctgacgaac
     2821 cggacgacgg agaaggcgat ccgcctcgcc tccgacctcg cgggcggcct cgctcccgtc
     2881 gcggcgctgt cgacgccggc cgccgtcgcc gccgcgagcg acgtcgtcgt ctcgtgcgtc
     2941 tccgacggcc ccgacgtgga ggaggtgcac ctcgggagga acgggacgat cgaaggggcg
     3001 cgacccggag cggtcgtcgt cgactgctcg acgatcgacc cgacggtggc ccggctcgtg
     3061 gccgcacgtc tcgccgagaa ggggatctcc ttcctcgacg cgcccgtctc cggcgggcag
     3121 aagggggcga acgaagggac gctgtcgttc ttcgtcggcg gcgaggcgga ggcgctcggg
     3181 aaggcgaggc cggtgctcga ggcgatgggg aggcggatca cgcacctcgg cccttcgggc
     3241 gccgggcagc tcgggaaggc gacgaaccag gtgatcgtgg cgggaacgct cgcggcggtg
     3301 tcggagggga tggcgttcgc gaaggccgcg gggctgccga tggaggcgat ccacgccgcg
     3361 ctgacggggg gcgccgcgaa ctcctgggcg ctcgaggtgc tcgggaagaa gatgctcgac
     3421 cgcgacttca agccggcgtt cgcgatcaag caccagcaaa aggacctcgc gatcgtgctc
     3481 cgcgccgcgc gggcgaacgg cgtccctctt cccgcggcct cgctcgtcca ccagctgctc
     3541 tcgtcgctcg aggcgaaggg gcgcggcgag gaggggacgc aggcgctcta cacgctctac
     3601 gaagagctgt cgcgctgacg cggagcggag cgcgcccgcc ggggcgcccg cggctcagcg
     3661 ggcgggcggc gggacccggg ccggggcggc ggcgacgccg gcccgccggc cgtcgacgat
     3721 tcccgtccag accccgcgcg cctcgtcgcg cgcgacgccg tagatacctc cgaagacgcc
     3781 gctgtcgcgg ctcggggcgc gaagcgccca gacgctcggg tagcccatcg cctcgagcgc
     3841 cgcgacgtcg cgctccgtga aaggcggcgc gagctcgagc atgacgcgcg ggccggcgag
     3901 gtcgtcctcc cagaccatcc gcggtcgggc gtaggcggcg gcgggcgtgt cgccgccgtc
     3961 gacgaacgca gtgacgaggc tcaggaggat ggaggggatc cggctgctcc cgggcgcgcc
     4021 ggcgatcagg gcgggacgat cgtcacggag aagcagaagc ggcgagacgg gcatcggcac
     4081 gacgccgcct gccttcagat actccgcaga ggcggggtcg tcggagacga gcggctcgag
     4141 gaacgcgttc aggaggaatc cgaggctcgg cgcggcccac gcggcgccgt agtagcggcc
     4201 gagggtctgg gtgagcgaga cgacgttgcc gtgggcgtcg gcgacggaca cgtgggtcgt
     4261 cccgcggatt cccgaggcga aggggagcgc gtcgccgagc tcggacggcg gcagcgccct
     4321 gtcggcgcga attcggcggg cctgccgctc ggcccaggcg ggcgaaagcc actcggagcg
     4381 aagcggcccc tccgggacct ccggctggcc ccggtgcgca gccgcctccg cccgcgcgag
     4441 gcggacggcc tcgacgatcg cgtggccgcg cgcgaggccg ggctcccgga ggcgctcctc
     4501 ctccagggcg ccgaggacct ggagagccat gacgaggacg cctccccctc ccggcgacgg
     4561 gacggagagc gcggtgaggc cgcggtagga gccacgcacg ggcgggacgt cgaggacgcg
     4621 gcccgggata cgggcgaggt ccggccgcgt gaggaagccg ccggccgcga ggacctcccg
     4681 ctcgagcgtc gcagcgatct cgcccgtgta gaagtcctcc agaccgcgct cggcgagacg
     4741 ggcgagcgtg cgggcgaggt tcgggagacg gacgcacgta ccgagggggg cgggcccggg
     4801 aatccccgag tcgtcgacgg gaccggtcag gtagacgggg tagaggacgt cggagtcgaa
     4861 gaggcggcgg cggtaatcgc cgaggtagcc atgctcgtag acctgcgtcc tgtagcccga
     4921 ttccgccgcc tcgatcgccg gggcgaggac ctcggcgaac ggcttcgtgc cgtagacacg
     4981 cagggcgtgc gcgagcgtgg cgacggtcgt cgggaccgcg gccgccatcg gcccccagag
     5041 gtcggctccg tcccgcgcgg cgcggagccg gcggcggtcg atccggatcg gcacgggagc
     5101 cgggcagaag acggcgcgct cctctccgga ggcggtgcgg aggacgagcc acgtctgccc
     5161 gccgaggccg gagccgccgg ggtcggccgc ggtgagcgcg aacgccgccg cgacggccgc
     5221 gtcgacggcg ttgccgccgg cctcgagcat ccgcacgccg gcccacgtcg cctccggcgc
     5281 ggaggtgacg accatcccga gcggcgagga gacgacggag cgcggccgga tcgattgctc
     5341 gaggagccgg acgcgctcgg cctcgtccgg cgcgtcggcg gcggaaaggg ggccggcgag
     5401 ggcggcgagg agagggacga ggaggccggc cgcgacgggc cggccgcggc ggcggagagc
     5461 tccgcacatc gcagggagaa gagcgcaacc gggcgccact gtcaacgagg cgctcggcgc
     5521 gcgtccctcg acacggcgcc gtgcgctcgt agaattcgct tcgcccccca gtacgacgtc
     5581 cggaggtccc atgaagcgcg ccgctctcgc tctcgccacg ctcttcgtcc tcgtcgccct
     5641 ctcgcccgcc gccgcggccg acccgggcga gcggaagagc tacacgaaga cgttcccgtt
     5701 cggcgccggg aaggagtcga aggtcgggat tcgccagggg ccggtgacga tcgagtcggt
     5761 gaggcttcgc aactggcccg acgccgacga cttcgccgac gccgagaacg acgcgggcga
     5821 gacgcacacg atggtcgtgg agttcgagta ctcgaaccgc gacgaggcgc gcgactggaa
     5881 gtgccgctac acggtgacgg tgccggggaa ggacggcggc tcctgggccg agaacgaccg
     5941 gaccgcgacg ctcgactccg gcaaggtcca cgacacgaac aaggtcttcg tgaggatgaa
     6001 gacccgcaac tacaagcggg cgacgtccta ccgggtgacg ttcgagatct ggaaggactg
     6061 acttggacgg ggaggacccg gaccgccggg tcctcccccg gcgccttcgg cgcctccctc
     6121 tccgcgcggg aaggctgcgc tgcgcgcccc gccggagcgc gaacggggag gacccggacc
     6181 gccgggtcct ccctcagcgc ccggccgcct ccgggaagag gagctcctga ggccggaggg
     6241 ccgggaggag gctctcgatc gcgcacttcg cccatccctc gcggcagacg gcgtcgagcg
     6301 cgacgaacgg aacgccccaa tcctcgagcg tcgcgaggag gagcgcgtcg atgtcgtgct
     6361 ggaaggcgag gtcggtgtcg cggacgccgt cgaagctcgg gtccccgacg atcggcaccc
     6421 ggaagagggc gtcgtaggtc gggagccagg ctttcacgag ctcctcgagc accttcacct
     6481 gcgggtcgcc cggggcggcc cgcttcaggt agcagtagtt gtcgaggacg gcccggtcgc
     6541 agacgacgac ctcggccttg gcctcggcct ggagctccca ggcgatctgc gtgtggagga
     6601 tccaggcctg cgccttgatc gtcgtgtcgc ggttgatggg gagcgggcac tcgcgcgcca
     6661 cctcgcgtac gagctcgacg gtcaggtcgc gccgcttcag cgcggcggcg aggtcgtagc
     6721 agagggtcgt cttgccgact ccgtgagtcc cgatgaacgc gatcttcatc ccgcccgatc
     6781 ctcgcacgaa ccgcgcgctt cgtctcgtcg cgacgagtct cagtacatga gcaggcatca
     6841 agagaagcta cggacgtgac ctggggggtc cgggggatcg cctctcgcgt ggcgcaagtg
     6901 acgacgcggg agatcgccgt ggcccggagt cagggggtcc ggggggtcgc gaacagcacc
     6961 ccccggagag cgcgcacccc ccgggaaggc tcagtgccgg gagggggcgg cgcgcgcgcc
     7021 cttcaggacc agatcgtccg gcggacgacg acgagggtcg tgtcgtcggc gggctcccct
     7081 tcgccgatgt gcgtgcgcca gtcggcgagg agcgcctcga ggatcgagtc ggcgggccgt
     7141 ctccagagcc cgatgcgctc gaggacgcct cgcgggcgct cgtagccgta cgggtcgtcg
     7201 aagcggtcgg ggccctcgaa gaggccgtcg gaggcgaaga cgaggctcgc cgcgcccggc
     7261 agctcgagct ccagctcgga gtaggtgcgc cccggcccct tcccgagcgg gaggccggag
     7321 acggagagct cgcggacgcg cccctcgacg acgaggagcg gccacgggtg cccggcgttc
     7381 gcgaggacga cgcgcccggt ggccggctcg atcctcacga ggacgagcgt cgtgaagagg
     7441 cgcgcggcgc gccccgcgcg gaggacgcgg tcgatctcgg cgaggacctc gcccggacgg
     7501 cgccccgcct tcacgagcga gaggaggcag gccttcacca tcgctccctg gatgccgcag
     7561 gagtagccgt gccccgcgac gtcgccggcc gcgacccaga gcgccccttc ctcgtcgacg
     7621 aggaactcgt acaggtctcc tcccgcctcg gtcgccacga tcgtccgcgc cgcgatctgg
     7681 agcccgggca cgtcgggcgg cgcgtcgggg aggagcgcga gctgcatccg cgagaggagg
     7741 tccatctcgt acttcacgcg ccgctcggcc tcgagccgct tcacgaactc gggtgcgtcg
     7801 acccgcgcct cgtcctcgcg ctccgggcgg cggctcgcga cgacgccgac gcacgcgagg
     7861 gcggccagcg acgccgccgt gaggaggagg gggacgaaga gctcccccgg gaagcgggag
     7921 gccgcgagga ggtcgcggaa gagggccggc gccgtcgccg cgacgaggag gccggtcagg
     7981 ccgtgggacc ggaaggcgag gaggaggaag gccgcctcgg cgagggtcag gacgaggagc
     8041 gctccccacg gggcgtagag gaaggagcag gagtagaaga gggcgaagag gacggcgccg
     8101 aggacgtcgg ccctctcggg cgggaggacg aaacgggcga gcgccgcgcc gaggaggagc
     8161 gtcgcggccg cgtagggacc ctcgaggaag gcgttcgcga tgccgccgaa aagggggagg
     8221 acgtaggacg gcgcctcggc gtagacgccg gggacccccg cggccgcgac ggacgaggcg
     8281 aagaggaggc ggaggcccgc gatggctccg ccgaggccga gcccaccgag gatcgccgac
     8341 ccgagccgcg gcgtgaggcg gcccgcgacg agcgcgtcga ggctcgtccg gaagcccggg
     8401 acggagtccc gcagcatcgc ctcggcgacg gcccagtagc cgacgagcgc gaccgcgacc
     8461 aggagacgcc cggtgacgat gaagcccgcc aggagtcccc gctgcccgcc gagctcgaag
     8521 agcgcgcccg agaggaccat ccccgcgacg ccgaggaggc cgagaccgat cgcgatccgg
     8581 aatccgagcc gcctccggaa gaggaggacg ccgaagacga cgaggactcc gaggccgacg
     8641 acgccgaaga gcgggatggc gatggcgagc ccccggaacg tgagcttctc gtagcgcgcg
     8701 ccgatgtcgg ggtcggcgag ctcccgggag aggacgagga tcgccgtcgt cggcgcgacg
     8761 cgcgtcacga cctctcgggg cccctcgcgg gacgggccga gctcccagac gcggacgatc
     8821 gtactgccgc tctcgtggtc cgtcggggcg ccgagcgcct ccccgcgggc gagtcgcccg
     8881 aggactcctg cggcgaagcg ctcgcgcgcg gcctgcacct ctccggaggg ggcggcgacg
     8941 cggaagacgc tcccgctcgt gaactccacg cggcggagcg tcccgtcgcg cgcgatggcg
     9001 acctccgccg gcccggtccc gacgccgggg acgcggagcg accccgagac gacccaggag
     9061 gcgagaccgc cgtggcgggc gagccaccgc ggcgcctccc ggccgaggcg acggtaggcg
     9121 cgctcgagct cgctcgaggc gaggccgttc ctcaccccgg ccttcggcgc gaggagcgtc
     9181 gcgccgaccg cggccatctc ggcgcgggcc gtcgcgatcg cggcctcctt cgagaccccg
     9241 agaggctgcc actcgggaag gacgaggtgc gccgcgacgg cgcccgccag gaggacgacg
     9301 aacccgccca cgagcgcccc cggggggacg cgcggcttcg gacgcggggc ggcggcttcc
     9361 gtcacgaagc cggtcggctc acgagcggcc gagcgcgggg acgaacgtct tccccgccgt
     9421 cagctcgagc atccggtcga gcgagcgctt cgccgcgagg cgcgtctcct ccggcacctc
     9481 gatcgcgggc gaaaggtccc ggagcgcgag ccagagcttc tcgagggtgt tccgcttcat
     9541 gtgcgggcag gcgttgcagg agcagccctc gtcgggcggc gccgcgagga gctccttgcc
     9601 cggcgcggcg cggcgcatct ggtggaggat cccctcctcc gtcgccacga tgaacgagcg
     9661 gtgctccgac tcggtgacgt gccggagcag cgccgaggtg gagccgacga agtgggcgtg
     9721 cgcgaggatc cgctcgtcgc actcggggtg ggcgacgagg cgggccgacg ggttcctcac
     9781 catcaggtcg aggatccgcc tctcggagaa cgtctcgtgg acgacgcacg tccccggcca
     9841 gacgacgaac ttccggccga gcttcttctc gagccaggcg gcgaggttct tgtcgggggc
     9901 gaagacgacc gggtcgggga tctggttcag gatcgcctcg gccgaggagg aggtgacgat
     9961 gacgtcggag ag
//
LOCUS       DAOTEZ010000123         9938 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4801_length_9938_cov_5.105636, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000123 DAOTEZ010000000
VERSION     DAOTEZ010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9938
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4801_length_9938_cov_5.105636"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..708)
                     /locus_tag="P5164_08150"
     CDS             complement(85..708)
                     /locus_tag="P5164_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43902.1"
                     /translation="MNTRRLLGVAFLTAFAAFGAALVLSARARGGDRLPAEVERGRRG
                     PASSGRTEAEEKAVEAASTGGLRAAEDEDAVESEARQELRDLYGDYRPYFVRGDFDSD
                     GRLDFAQAFVRREGPEPLFDVAAFFGAEDGGFRPSVWVDRGLLLSGGDLSIDRSVLVV
                     SEDVEQDVSRRWRWEPSAGRFVDVDAGAGEGDEAPWVEPDLRTGTTA"
     gene            complement(705..1487)
                     /locus_tag="P5164_08155"
     CDS             complement(705..1487)
                     /locus_tag="P5164_08155"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRY43903.1"
                     /translation="MPSRRPRRNDALSAALGYFRDLGVRELHLAAPPAIEAPASARPG
                     AVPDEGVAVPSSRPASVRGPAVPDLPDLDALRATVAACELCGLCRTRTQTVFADGTGR
                     SRIMFVGEAPGADEDATGVPFVGRAGQLLTKMIGAMGLDRADVYIANVLKCRPPGNRN
                     PEPAEIAACRPYLEAQIEAVKPAVLVAMGKFAAQFLLETEEPITRLRGRWGTRQGIAV
                     MPTFHPSFLLRQPERKKEAWEDLQQVLRKLGLPVPGRKEAGP"
     gene            complement(1451..2698)
                     /gene="coaBC"
                     /locus_tag="P5164_08160"
     CDS             complement(1451..2698)
                     /gene="coaBC"
                     /locus_tag="P5164_08160"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRY43904.1"
                     /translation="MSGGGLPAPRVLLGVSGGIAAYKAAEIVRLLVEKGAVVRVLMTP
                     AAQRFITPLTLSVLSKAPVVADLWDPASGAVDHVELARWGEVLAVAPATADVLAKMAR
                     GIGDEALSTYALAHRRAIVVAPAMNTWMWAHPATQENLRILRGRGAVVVDPESGDLAC
                     GDVGPGRMAPPARIVDAVVGAARVSRSLQGRRVVVTAGPTREPIDPVRFLTNRSSGRM
                     GYALAREAEKRGAEVVLVSGPVAIDPPPNVKLVKVDRTAEMLEAVLAELPSSDALVMA
                     AAPADFQAEAFTPKKIKRAGGIPEIRLALAPDILAAVRAVRTPRQVVVAFAAETDDLA
                     GNARRKLLDKGADLLVANDVSRPGIGFDSDENEVLLLAAGASDPVAVPRAAKAVVSAT
                     ILERLAALLDERCPPAAPAETTR"
     gene            complement(2707..3021)
                     /gene="rpoZ"
                     /locus_tag="P5164_08165"
     CDS             complement(2707..3021)
                     /gene="rpoZ"
                     /locus_tag="P5164_08165"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRY43905.1"
                     /translation="MTTPNEETGASPMTETPEETPESPEIESKFRLVHIASRRAEQLM
                     LGARPKVETKHTKYSRVALDEVAGGHVKWQLGQRPAEGLVEGEPDPGAIEPIDPLPLI
                     TP"
     gene            complement(3044..3670)
                     /gene="gmk"
                     /locus_tag="P5164_08170"
     CDS             complement(3044..3670)
                     /gene="gmk"
                     /locus_tag="P5164_08170"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRY43906.1"
                     /translation="MRPAGDLFIVSSPSGAGKTTLIRRILADPAFVGTIHFSVSHTTR
                     PARPGEVHGREYYFVSPEEFESLASRDGFLEHAVYDGHRYGTSRGEVEPRLAAGIDVV
                     LDIDVQGARQVRSRMPEVVKVFVFPPSRQVLEERLTARGHNTRDSIARRLGAAARELA
                     EFGEYDYAIINDILDAAVDELRSIVVARRSSRRRRQERLEAILRTFPA"
     gene            complement(3759..4619)
                     /locus_tag="P5164_08175"
     CDS             complement(3759..4619)
                     /locus_tag="P5164_08175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HRY43907.1"
                     /translation="MTGFGRAQAALPDGTQLSITLRSVNHRFLDLSLKMRDDLGALEA
                     PLRKLVGATVARGHVDLSVRVTRSTGGAVSIDGAAAARYAEAWDALSKERGLPRELTA
                     RDLLSLPGVVRVGEDEAGEALGGAVLETAAAALADFDASRRREGEALATALTAILDRL
                     EAGIGKVSEAREGLAERLLAQLRERVAKLADGVPLDEARLAQEIALLADRADITEEVD
                     RFRAHLVEARRLMRGEHASGKRLDVLAQELHREANTASQKARELPLTRVLLDLKSDVE
                     ALKEQIQNVE"
     gene            complement(4625..6514)
                     /locus_tag="P5164_08180"
     CDS             complement(4625..6514)
                     /locus_tag="P5164_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRY43908.1"
                     /translation="MRDFLRLARLFRPHARAALVAVLAMVGVAVFTTLVAYLFGPLFD
                     QVLTPGQNAAVKQELSADPAARGLGKALGAAETARKTPVIQWLDDRLLDVQSAVGVTE
                     ANRGVVLPVILLVAFVLKNLCAFAAEYRFNAVGLAFVRDVRRLVYERLLGQSGAFHAK
                     NPSGDLISRVTGDVDRIQSLFGTDLADLVQSIATLIGLSVLVVSRSPELTLVALVVAP
                     AIVVPVVLIANRLRRISKAGRERMGDLTGVLSETLRGHRVVQAYGAERYEADRFGEVN
                     ERTFRLLRKGAGVMALSSPLVETASVLAFLVLLGYAGKRIEAGTMTLGAFISFGVGLV
                     MMYQPFKRATRINLALQQALASARRLFEVLDAPVLVVDRPDAAPLRPFEKEVVFEGVS
                     FAYPGGPDVLSGVDLAVPRGSVTALVGPSGAGKTTLANLLPRFMDVTAGRITIDGVDV
                     RDVTLASLRGAMALVTQEVVLFDDTVRRNVAYGLADVPESRVREALAAANAADFVDAL
                     PKGLDTPVGEAGGRLSGGQRQRLAIARALLKDPPILILDEATSALDTESERAVQAALE
                     RLMAGRTTLVIAHRLSTVRRADQIVVLDRGRLVERGRHAELLALGGVYRRLHDLAAFE
                     EEKTP"
     gene            complement(6511..7656)
                     /gene="lpxB"
                     /locus_tag="P5164_08185"
     CDS             complement(6511..7656)
                     /gene="lpxB"
                     /locus_tag="P5164_08185"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HRY43909.1"
                     /translation="MRLLLSAGEVSGDQHGAALLGALAERVSGLEAFGLAGDRCAARG
                     MRLLAHQKELAIIGVVEALGKIRFAHRLMRDLTREAVASGVDAAVLIDSPDFHLPLAR
                     RLAKAGVPVVFYVSPQVWAWRSGRARTIARLGRGVLTLFRFEKRWYDARGLGERVIWV
                     GHPLVDEARRELATPMLPPPPGRRRVVLMPGSRAGEVRTLLPILREAVALLEARRSDL
                     EIVLVKADSVPDDLLRRIAGASLDRWSVVAGPHLSLLAASDVLIVASGTATVEGLLAG
                     IPMVVVYRVAAISYLLGKLLVKVPSVAMANILSDPGDGSRAVPELIQGAATPERVAAE
                     VERFLDDRERAAEARRRLAAGRAELGEPGAPARTAEAVLALLGRSRS"
     gene            complement(7661..7852)
                     /locus_tag="P5164_08190"
     CDS             complement(7661..7852)
                     /locus_tag="P5164_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43910.1"
                     /translation="MRTAPLADHFLHLVLLATILSAFFSILWRDDGPSRRRFFARMWT
                     AIVGGSLAAAWAMSVVGSR"
     gene            complement(7849..8193)
                     /locus_tag="P5164_08195"
     CDS             complement(7849..8193)
                     /locus_tag="P5164_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43911.1"
                     /translation="MLATLLVPGLGHALCGRRGRGAAYFGLVGAAFVLGLALGAAPGP
                     IPARGLGAILTVSATHAVVLPALVVRMSGRGPGDPSSPSFEVATTYLVVAGVLNVLLA
                     FDAWDVAGGRKP"
     gene            complement(8232..8735)
                     /locus_tag="P5164_08200"
     CDS             complement(8232..8735)
                     /locus_tag="P5164_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012123544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HRY43912.1"
                     /translation="MNDVLFTPWRMSYLTGAPPSGAGGCLFCDLPGLGDAEALIVERG
                     RSVFAVLNRYPYSNGHVLVAPYAHRATLPDLTREERIDLIDLSARVEEALRAEYAPGG
                     LNAGLNLGKCAGAGVPGHLHMHLVPRWDGDTSFLTVVSGTRVVPEDLDVTFRRLRARL
                     GSPEGTP"
     gene            complement(8732..9100)
                     /locus_tag="P5164_08205"
     CDS             complement(8732..9100)
                     /locus_tag="P5164_08205"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRY43913.1"
                     /translation="MERESQAASRAEETTTKADLVEEVARTAQLTKKQAEAVVESVFQ
                     GIISSLRAGSKIELRGFGSFKLRQRGARIARNPKRSGVKVHVPPKLVPYFKPGKKLKE
                     LINAAAPEPEPAAGARAPEE"
     gene            complement(9171..>9938)
                     /locus_tag="P5164_08210"
     CDS             complement(9171..>9938)
                     /locus_tag="P5164_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRY43914.1"
                     /translation="DVNPETRRLSLSLRATEPNPWEQLAEKFRIGDRVQGVVRNLTDF
                     GAFVEIEEGIDGLVHISDMSWGRRVKHPSEVLKKGDQIQAVLTAIDVENRRISLSIKE
                     FRPNEWEEFSREHQPGDVVDGQVAKVADFGVFVRLPLGLEGLMHVSETDVLRGQKLSD
                     VFSEGDPVRVRILRIEVDEQRIGLSMRDVPQPDPAELAAAAAPQSEASPEAASEEEAA
                     SGEPAAASDAGASVEPAAEEPPPAVAEASAEAPKTED"
BASE COUNT         1414 a         3544 c         3676 g         1304 t
ORIGIN      
        1 gggagcaggt acgtggcgag tcccggaaac ccggtcggaa ggaggaccga gactcgccgg
       61 atgacgtcgt cggacgccac ggcgtcaggc cgtcgtgccg gtcctgaggt ccggctcgac
      121 ccagggggct tcgtcgccct cgccggcccc cgcgtcgacg tcgacgaaac ggcccgcgga
      181 tggctcccag cgccacctgc gggagacatc ctgctcgacg tcctccgaga cgacgagcac
      241 ggagcggtcg atgctcaggt cccccccgga gaggaggagc cccctgtcga cccagacgga
      301 gggccggaac cccccgtcct ccgcgccgaa gaaggcggcg acgtcgaaca ggggctcggg
      361 cccctcccgg cggacgaagg cctgcgcgaa gtcgagccgg ccgtcggagt cgaagtcgcc
      421 tcgaacgaag tacggccgat agtcgccgta gaggtctcgc agctcctggc gcgcctccga
      481 ctcgacggcg tcctcgtctt cggccgcccg caggccaccg gtcgacgcgg cctcgacggc
      541 cttctcctcg gcctccgtcc gcccgctcga ggcaggcccg cggcggcccc gctcgacctc
      601 ggccgggagt cggtctccgc ctcgcgcccg ggccgacagg acgagcgccg cgccgaaggc
      661 ggcgaaggcc gtcaggaacg cgaccccgag gaggcggcgg gtgttcacgg gccggcttcc
      721 ttcctccccg ggacggggag gcccagcttg cggaggacct gctggaggtc ctcccacgct
      781 tccttcttcc gctcgggctg gcggaggagg aaggaggggt ggaacgtcgg catcaccgcg
      841 atcccctgac gtgtccccca ccttccgcgg agccgcgtga tcggctcttc tgtctcgagg
      901 aggaactgcg ccgcgaactt gcccatggcg acgagcaccg cgggcttcac ggcctcgatc
      961 tgggcctcca ggtagggccg gcaggcggcg atctccgcag gctcggggtt ccggttcccc
     1021 gggggccggc acttcaggac gttcgcgatg tagacgtcgg cgcggtccag tcccatcgcc
     1081 ccgatcatct tcgtcaggag ctgtccggcg cgaccgacga acggaacgcc cgtcgcgtcc
     1141 tcgtcggcgc cgggggcctc cccgacgaac atgatgcggc tgcgcccggt cccgtccgcg
     1201 aacacggtct gcgtccgcgt gcggcagaga ccgcagagct cgcacgcggc aaccgtggcc
     1261 cggagcgcat cgaggtcggg aaggtccggg acggccgggc ccctcacgga tgcgggtcgc
     1321 gaggacggca cggcgacccc ctcatccggg acggcgccgg gccgcgcgga cgccggcgct
     1381 tcgatcgccg gaggcgccgc gaggtggagc tcgcggacgc cgaggtcccg gaagtagccg
     1441 agcgcggcgc tcagcgcgtc gtttcggcgg gggcggcggg agggcatctc tcgtcgagga
     1501 gcgccgcgag gcgctcgagg atcgtggcgg aaacgaccgc tttggcggcg cgggggacgg
     1561 cgaccgggtc ggacgcgccg gcggcgagaa ggaggacctc gttctcgtcg ctgtcgaagc
     1621 cgattcccgg acgggagacg tcgttcgcga cgaggaggtc ggcccccttg tcgaggagct
     1681 tacgacgcgc gttcccggcg agatcgtcgg tctcggcggc gaaggcgacg acgacctgcc
     1741 ggggcgtcct cacggcacgg acggccgcga ggatgtcggg ggcgagggcg agccggatct
     1801 cggggatccc gccggcccgc ttaatcttct tcggcgtgaa ggcctcggcc tggaagtcgg
     1861 cgggcgccgc cgccattacg agggcgtcgg aggacgggag ctcggcgagg acggcttcga
     1921 gcatctcggc cgtccggtcg actttcacga gcttcacgtt cgggggcggg tcgatggcga
     1981 ccgggccgct cacgaggacg acctccgcgc cacgcttctc ggcctcgcgc gcgagggcgt
     2041 atcccatccg tcccgagctc cggttcgtca ggaagcggac cgggtcgatc ggctcgcggg
     2101 tcgggccggc cgtgacgacg acccgcctcc cctggaggct cctcgagacc cgtgcggcgc
     2161 cgaccaccgc gtcgacgatc cgcgcgggcg gagccatccg gccgggaccg acgtcgccgc
     2221 aggcgaggtc ccccgattcg gggtcgacga cgacggcgcc gcgcccgcgc aggatccgga
     2281 ggttctcctg cgtcgcgggg tgggcccaca tccaggtgtt catcgccggg gccaccacga
     2341 tcgcccggcg gtgcgcgagc gcgtaggtcg agagcgcctc gtcgccgatc cctcgggcca
     2401 tcttcgcgag gacgtccgca gtcgccggtg cgaccgccag gacttccccc caccgcgcca
     2461 gctcgacgtg atcgacggcc ccggaggcgg ggtcccagag gtccgccacg accggcgcct
     2521 tcgaaaggac cgagagcgtc agcggcgtaa tgaaccgctg ggcggccggc gtcatgagca
     2581 cccgcacgac ggcgcccttc tcgaccagca gccggacgat ctcggccgcc ttgtaggccg
     2641 cgatgccgcc gctgacgccg agaaggacgc gcggggccgg gaggccgcct ccgctcacgc
     2701 cggccgtcag ggcgtgatca gcgggagcgg gtcgatcggc tcgatcgcgc ccggatcggg
     2761 ctctccctcg accagccctt cggcggggcg ctggccgagc tgccacttca cgtggccgcc
     2821 ggccacctcg tcgagggcga cgcgggagta cttcgtgtgc ttcgtctcga ccttcggacg
     2881 agccccgagc atcagctgct cggcacggcg ggacgcgatg tggacgagcc ggaacttcga
     2941 ctcgatctcc ggggattccg gggtctcttc gggggtctcg gtcatcgggc tggctccggt
     3001 ctcttcgttc ggtgtcgtca ctttcaccct cgattcggcc ccgtcacgcg gggaacgtcc
     3061 gcaggatggc ttcgagccgc tcctgccgcc ggcggcggct cgaacggcgc gccacgacga
     3121 tcgaccgcag ctcgtccacg gccgcgtcga ggatgtcgtt gattatcgca tagtcgtatt
     3181 cgccgaactc ggcgagctcc cgcgcggcgg cgccgagccg gcgcgcgatc gagtcccgcg
     3241 tgttgtgccc ccgggcggtc agacgctctt ccaggacctg gcgggagggc gggaagacga
     3301 agaccttcac gacctccggc attcgggacc gcacctgacg ggctccctgg acgtcgatgt
     3361 cgaggacgac gtcgatcccg gccgcgaggc gcggctcgac ctctccgcgc gaggtcccgt
     3421 agcggtgccc gtcgtagacc gcgtgctcga ggaagccgtc ccgggaggcc agcgactcga
     3481 actcctccgg cgagacgaag tagtactccc ggccgtgcac ctccccgggc cgcgccggcc
     3541 gggtcgtgtg cgagacggag aagtggatcg tcccgacgaa ggccgggtcg gcgaggatcc
     3601 gtcggatcag ggtcgtcttg cccgcgccgg acggggacga gacgatgaag aggtctcctg
     3661 cgggtctcat ccccagagta tgcgaggtac ccgaccgcca gtcagcagga acgcgctcgg
     3721 ggggtccggg gggcggcgaa cagcgccccc cggcagcgtc actccacgtt ctggatctgt
     3781 tccttgagcg cctcgacgtc cgacttgagg tcgaggagga ctcgagtgag cggaagctcg
     3841 cgggccttct ggctcgccgt gttcgcctcg cggtggagct cctgcgcgag gacgtccagg
     3901 cgcttcccgg aggcgtgctc tcccctcatc agcctgcgcg cctcgacgag gtgcgcccgg
     3961 aagcggtcga cctcttccgt gatgtcggcc cggtccgcga ggagagcgat ctcctgggcg
     4021 agccgggcct catcgagcgg gacgccgtcg gcgagcttcg cgacgcgctc gcggagctgc
     4081 gcgaggagac gctcggcgag cccttcgcgc gcttcggaga cctttccgat ccccgcctcg
     4141 agccggtcca ggatcgcggt gagagcggtc gcgagcgcct cgccctcccg ccgccgcgac
     4201 gcgtcgaagt cggccagcgc ggccgccgcg gtctcgagga cggcaccgcc gagcgcctcg
     4261 ccggcctcgt cctcgccgac gcggacgacg cccgggagcg agaggaggtc gcgcgccgtc
     4321 agctccctcg ggagcccccg ctccttcgag agggcgtccc aggcctccgc gtaccgtgcc
     4381 gccgccgccc cgtcgatcga gacggcgccc ccggtcgagc gtgtgacgcg gaccgagagg
     4441 tcgacgtgcc cgcgggccac cgtcgcgccg acgagcttgc gcaggggcgc ctcgagcgct
     4501 ccgaggtcgt ccctcatctt gagcgagagg tcgaggaagc ggtggttcac gctgcgcagc
     4561 gtgatcgaga gctgcgtccc gtcggggagc gcggcctggg cgcggccgaa gccggtcatc
     4621 gacttcacgg tgtcttctcc tcctcgaagg cggcgaggtc gtgcagcctc cggtagacgc
     4681 cgccgagcgc gaggagctcg gcgtgccggc ccctctcgac gaggcggccg cggtccagga
     4741 cgacgatctg gtcggcccgc ctcaccgtcg agaggcgatg ggcgatgacg agcgtcgtcc
     4801 gccccgccat cagccgctcg agcgcggcct gaacggcccg ctcgctttcg gtgtcgagcg
     4861 cggaggtcgc ctcgtcgagg atcaggatcg gcgggtcctt caggagcgcc cgcgcgatcg
     4921 cgaggcgctg ccgctgccct ccggagagcc gtccccccgc ctccccgacc ggcgtgtcga
     4981 gccccttcgg gagggcgtcg acgaagtcgg cggcgttcgc ggcggcgagg gcctcgcgga
     5041 cgcggctctc cggcacgtcc gcgaggccgt aggcgacgtt cctccggacg gtgtcgtcga
     5101 acaggacgac ctcctgcgtc acgagcgcca tcgccccgcg cagggaggcg agcgtcacgt
     5161 cgcggacgtc gacgccgtcg atcgtgatcc gccccgcggt cacgtccatg aagcgcggga
     5221 ggaggttcgc gagcgtcgtc ttccccgccc ccgacggacc gacgagggcc gtcaccgagc
     5281 cgcgcgggac ggcgaggtcg acgcccgaga ggacgtccgg cccgcccggg taggcgaacg
     5341 agaccccttc gaagacgacc tccttctcga acggccggag cggcgcggcg tccgggcggt
     5401 cgacgacgag gacgggggcg tcgaggacct cgaacaggcg ccgcgcggag gcgagcgcct
     5461 gctggagcgc gaggttgatc cgcgtcgccc gcttgaacgg ctggtacatc atgaccagcc
     5521 cgaccccgaa cgagatgaac gccccgagcg tcatcgtccc cgcctcgatc cgcttccccg
     5581 cgtagccgag aaggaccagg aaggcgagga ccgaggccgt ctcgacgagc ggcgaggaga
     5641 gggccatgac gccggcgccc ttccgaagca gccggaacgt ccgctcgttc acctcgccga
     5701 agcgatccgc ctcgtaccgc tccgccccgt aggcctggac gacgcggtgg ccgcggagcg
     5761 tctcggagag gacgccggtc aggtcgccca tccgctcgcg tcccgccttc gagatccgcc
     5821 ggagccggtt cgcgatcagg acgaccggga cgacgatcgc cggcgcgacg acgagggcga
     5881 cgagggtcag ctcgggagat cgcgacacga cgaggaccga gagaccgatg agcgtcgcga
     5941 tcgactggac gaggtcggcg aggtcggtcc cgaacaggct ctggatccgg tcgacgtccc
     6001 ccgtcacgcg cgagatgagg tcccccgatg ggttcttcgc gtgaaaggcc cccgactgac
     6061 cgaggagccg ctcgtagacg aggcggcgca cgtccctcac gaacgccagc ccgaccgcgt
     6121 tgaaccggta ctcggcggcg aacgcgcaga ggttcttcag gacgaacgcg acgaggagga
     6181 tgaccggcag gacgacgccc cggttcgcct cggtcacgcc cacggcggac tggacgtcca
     6241 gcaggcggtc gtcgagccac tggatgaccg gcgtcttgcg cgccgtctcg gccgcaccga
     6301 gcgccttgcc gaggccgcgc gccgccgggt cggcagacag ctcctgcttc acggccgcgt
     6361 tctgccccgg cgtcaggacc tggtcgaaga gcggaccgaa aaggtaggcg acgagcgtcg
     6421 tgaagacggc cacgccgacc atcgcgagga cggccacgag cgccgctctc gcgtgcgggc
     6481 ggaagaggcg cgcgaggcgg aggaagtccc tcaagaacgg ctccgtccga gcagcgcgag
     6541 gacggcctcg gccgtccggg ccggggcgcc cggctcgccg agctccgccc ggccggcggc
     6601 gaggcggcgc cgggcctcgg cggcgcgctc ccggtcgtcg aggaagcgct cgacctcggc
     6661 cgccacacgc tcgggcgtcg cggctccctg gatcagctcc ggcacggcgc gcgagccgtc
     6721 gcccggatcg gagaggatgt tcgccatcgc gacgctcggc accttcacga ggagcttgcc
     6781 cagcaggtag ctgatcgcgg cgacccggta gacgacgacc atagggatcc cggcgaggag
     6841 cccctcgacc gtcgcggtgc cggaagcgac gatcaggacg tcggacgcgg ccagaagcga
     6901 gaggtgcgga ccggcgacga cgctccagcg atcgaggctc gctccggcga tccggcgcag
     6961 gaggtcgtcg ggaaccgagt cggccttcac gaggacgatc tcgaggtcgg acctccgcgc
     7021 ctcgaggagg gcgacggcct cccgcaggat cgggaggaga gtccgcacct ccccggcgcg
     7081 cgagccgggc atcaggacga cgcgccggcg gccgggcggc gggggaagca tcggcgtcgc
     7141 cagctcgcga cgggcctcgt cgacgagagg gtggcccacc cagatcacgc gctctccgag
     7201 gccgcgcgcg tcgtaccagc gcttctcgaa ccggaagagc gtcaggacgc cgcggccgag
     7261 acgcgcgatc gtccgcgccc gcccgctcct ccaggcccag acctgaggcg acacgtagaa
     7321 gacgaccggg accccggcct tcgcgagccg ccgggcgagc gggagatgga agtcgggaga
     7381 gtcgatcagg accgcggcgt cgactcctga cgcgacggcc tcgcgcgtga ggtcgcgcat
     7441 gaggcggtga gcgaagcgga tcttcccgag cgcctcgacg acgccgatga tcgcgagctc
     7501 cttctggtgg gcgaggaggc gcatgccgcg cgccgcgcac cggtccccgg cgagaccgaa
     7561 cgcctcgagc ccggagacgc gctcggccag cgcgccgagg agcgccgccc cgtgctggtc
     7621 gccggagacc tcgccggccg acaggaggag tcgcacggag tcagcggctc ccgacgaccg
     7681 acatcgccca ggcggccgcg agcgagccgc cgacgatcgc cgtccacatg cgcgcgaaga
     7741 agcgccgccg ggagggtccg tcgtcgcgcc agagtatcga gaagaaggcc gagaggatcg
     7801 tggcgagaag gacgaggtgg aggaagtggt cggccagcgg agccgttctc acggcttccg
     7861 cccccctgcg acgtcccagg cgtcgaacgc gagaaggacg ttcagaacgc ccgcgacgac
     7921 gaggtaggtc gtcgccacct cgaaggaggg cgaggagggg tctcccgggc cccgcccgga
     7981 catcctcacg acgagcgccg gcaggacgac ggcatgggtc gcggatacgg tcaggatggc
     8041 gccgagccct cgcgccggga tcggcccggg ggccgctccc agcgccaggc ccaggacgaa
     8101 ggccgcgccg acgagcccga agtaggccgc cccgcgcccg cgtcggccgc aaagagcatg
     8161 gccgaggccc ggaacgagaa gcgtggccag caccgcgagg aggcgccggc cggtcgggcg
     8221 ccgcgcgccc gtcagggggt cccctccggc gagccgaggc gcgcgcggag acggcggaac
     8281 gtgacgtcga ggtcctcggg gacgacgcgc gtcccggaca cgaccgtcag gaagctcgtg
     8341 tctccgtccc agcgcggcac gaggtgcatg tgcaggtggc cgggaacgcc ggctcccgcg
     8401 cacttcccca ggttcaggcc cgcgttcagg ccgcccggcg cgtactccgc ccgaagagcc
     8461 tcctcgacgc gcgcggagag gtcgatcagg tcgatccgtt cctcccgcgt caggtcgggg
     8521 agggtcgcac gatgggcgta cggagcgacc aggacgtgtc cgttggaata gggatacctg
     8581 ttcaggaccg cgaagacgct ccggcccctt tcgacgatca gggcctcggc atcgccgagg
     8641 cccgggaggt cgcagaagag gcagcctccg gcgcccgatg gaggtgcgcc cgtcaggtag
     8701 ctcatccgcc agggcgtgaa gaggacgtcg ttcattcctc cggggctcgg gcgcccgcgg
     8761 ccggctcggg ttccggggcc gcggcgttga tcagctcttt cagcttcttc cccggcttga
     8821 agtacgggac gagcttcggc ggcacgtgga ccttcacgcc gctgcgtttg ggatttcgag
     8881 cgatgcgggc gccccgctga cggagcttga agctgccgaa gccgcggagc tcgatcttgc
     8941 tgccggcgcg cagcgaggag atgatgccct ggaagaccga ttcgacgacg gcttcggcct
     9001 gcttcttcgt cagctgggcc gtccgcgcga cctcctcgac gaggtccgcc ttggtggtcg
     9061 tctcctccgc ccggctcgcg gcctgcgatt cgcgttccat ctcttcccct cgcggagggc
     9121 gtccccttcg gacgccctcc ggcggcgcct cctcgcaggc gcctcgaacg tcagtcttcc
     9181 gtcttcggcg cctcggcgga ggcttcggcc acggcgggag gcggctcctc ggcggccggc
     9241 tcgacggagg cgcccgcatc ggacgccgct gccggctcgc ccgaagcggc ttcctcctcg
     9301 gaagcagcct ccggagaagc ctcgctctgg ggtgcggccg ccgcggcgag ctccgccggg
     9361 tcgggctgcg ggacgtcccg catggaaagc ccgattcgct gctcgtcgac ctcgattcga
     9421 aggatgcgga cccgcacggg atcgccctcg gagaagacgt ccgagagctt ctgcccgcgc
     9481 aggacgtcgg tctcggagac gtgcatcagc ccttcgaggc cgagaggcag gcggacgaag
     9541 acgccgaagt cggcgacctt ggcgacctgc ccgtcgacga cgtcgccggg ctgatgctcg
     9601 cgggagaact cctcccactc gttcgggcgg aactccttga tggagaggct gatccgccgg
     9661 ttctcgacgt cgatggcggt gagcacggcc tggatctggt cgcccttctt caggacctcg
     9721 gacgggtgct tgacgcgccg gccccaggac atgtcggaga tgtggacgag gccgtcgatc
     9781 ccctcttcga tctcgacgaa cgcgccgaag tcggtcaggt tccggacgac gccctgcacg
     9841 cggtcgccga tgcggaactt ctcggcgagc tgctcccagg ggttcggctc cgtcgcgcgc
     9901 agcgagagcg agaggcggcg ggtctcgggg ttgacgtc
//
LOCUS       DAOTEZ010000124         9929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4810_length_9929_cov_8.142799, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000124 DAOTEZ010000000
VERSION     DAOTEZ010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9929
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4810_length_9929_cov_8.142799"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="P5164_08215"
     CDS             complement(<1..716)
                     /locus_tag="P5164_08215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HRY43915.1"
                     /translation="MRVVVTGGTGFLGRALVAALAGRGDEAVVVTRDPSRANLPPGAR
                     GVGWDGLATAVDGANAVVNLAGETIAQRWTASAKARIVGSRAQAAERAGAAVRAAKSP
                     PGVLVNASAVGFYGNRGDEELTEESPPGSGFLAETTLAWERAAREAVPEGIRLVLLRT
                     GVVLDPAEGALAKMLFPFRLGLGGPLGGGAQWMPWIHRDDLVALLLAALDDPRYEGPV
                     NGTAPGPVPMKDFAAALGRVL"
     gene            complement(721..1029)
                     /locus_tag="P5164_08220"
     CDS             complement(721..1029)
                     /locus_tag="P5164_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43916.1"
                     /translation="MSNAPKSSFELAMERLRAADRESGVEEKPLTDAQKAAIAEARSV
                     ASSRLAEREILFRDALKKVYDPVEREKAEEEYRVDRTRIEADRDRAIERIRRGEGERS
                     "
     gene            1053..1967
                     /locus_tag="P5164_08225"
     CDS             1053..1967
                     /locus_tag="P5164_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43917.1"
                     /translation="MSRHLARRTAIVGLALLAAAPSLGAARQGAEALVPMSAEELIRS
                     VARAQRRADLAVAASTFEQVEVKTDFGRDGRPKAIHRRRFRYTAGDGSAVRRELLEVD
                     GRPPTEAEIAEVAEEDAKEHRRRVDRRAAHRASRPPEVGGSEEDPLLGPIRLSDLISR
                     FSYGPVEEVLVDGRPAYGLDFAPRPGRVATRTLDRALGSLAGRVLVDATDLQVVSVDA
                     RLVTPLRVGGGLAASIRSASIAYRAQRLADGAWLPCLVGIRLQGRTGVLFRLDSAYRF
                     EFSDYARFLVDVETEVGRPGGPSVQSAP"
     gene            1964..2536
                     /locus_tag="P5164_08230"
     CDS             1964..2536
                     /locus_tag="P5164_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43918.1"
                     /translation="MTPSKRRLVLGLVLVGAFAGFFLAFRTALVPATHADHDHGILNL
                     DAGGYLEVLTRGGKKRNLVGAPGRVLAVTFVDPADPAAAEELRGLFAFQEATKGSEEA
                     EVVVLVKAASFAELDAWLARSALVPPVPASLTVDPEGKTTVKFNNRRPVETMFFGPDG
                     KLASQARGPLDWSLEAPRRIAAAAAGESIH"
     gene            complement(2683..4440)
                     /gene="sppA"
                     /locus_tag="P5164_08235"
     CDS             complement(2683..4440)
                     /gene="sppA"
                     /locus_tag="P5164_08235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRY43919.1"
                     /translation="MAKGGKGLLVLVLAIVVLGAGLVVAGVFLAGRKTPSVPSRAILE
                     LDLERGLPETAPDDPFAVFGGERPLTLRDVLDALEKAGDDPHVVGLVARLGAAPIGVA
                     EIQEVRDAVKAFRAKKKLAFAYAETFGEFAPGNGAYYLATAFDEIWLQPSGDVCLNGL
                     VAESPFVRGTLDKLGVKPEFGQRYEFKNAMNMYTETKFTDAHREATKALVDDLFGQMV
                     RGISEGRQMSEEEVRALVDRGPFLGPEALEAKLVDGLAYRDEVHEKLMKKGGAGAKLF
                     GLPAYLKKEGRPHDAGRKTIALVYGVGGVVRGKSRTNPVTGGRTMGSESVAKAIRNAV
                     ADDDVAAILFRVSSPGGSYVASDTIWREVVLAQKAGKPVVVSMGEVAASGGYFVAMPA
                     SKIVAQPGTITGSIGVLGGKMVTTAMWEKVGLTFDQVDAGANANLWNASRSFSPAEWE
                     RFNAWLDRVYVDFTSKVADGRKLPKEKVLEIAKGRVWSGEDAKALGLVDELGGYAVAL
                     RLAKEAAKIPASEKVKVAVFPKEKSPFEALTAMLSGEQEDESSFTALETVLGPLRPLL
                     RQIEAEQAGVLAAPVPTIR"
     gene            complement(4548..6029)
                     /locus_tag="P5164_08240"
     CDS             complement(4548..6029)
                     /locus_tag="P5164_08240"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HRY43920.1"
                     /translation="MPSGPGARRAILVSTLSVLAGSGVALVGSLVVRVLMARALEPDA
                     LGLVLLGIAVVTPVGSIAGLGTNAAMAQRLAELRAGGEETAARRIGGKALLLVSASGL
                     AATLLLALLAGPLARLLHQPGIDHVLLPMAPVALGLAAGAAALGVSRGFGDSLGRALV
                     RDAGGGLLRVAGVGTALLAGTASSFAVAAGFAAGSIAAELLFVAYVARKGWLSAPPAH
                     AGGPVALPDLRPFAANEVLTQTALWLDVVVLGALAPATAVGLYGVARGLTRVLDLVRQ
                     ASSHGYLPSAASAVAEGDEARLARLHVATRHLAFALVWPVLSVCLLSPGPLLVLLFGD
                     DYAPAAGALRLLALASFLPSFFDFLDLLLVADRRPGDVLRASIAGAAVLVLLLIVLAG
                     RLGGEGAALALLGAGLVRGFLLHRLVFRGRPIGPYLAAVAGPALGAISSLSAGGLLLA
                     LLAPGPHGALLVAAGTGAAASGASLLAFFRRREVRGTPSAPEA"
     gene            6110..6709
                     /locus_tag="P5164_08245"
     CDS             6110..6709
                     /locus_tag="P5164_08245"
                     /inference="COORDINATES: protein motif:HMM:NF019247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HRY43921.1"
                     /translation="MSLVRWMTLPALVLAFHASAQEKPTSAPAAAAEEAMKAWAAYAT
                     PGEAHARLAKLEGTWDVKTKSWMDPSQPPEETTGSCDFRMVLGGRYLEQRFQGTAMGQ
                     PFSGIGYTGFDNVKKKYEAYWIDSMGTGMMVMSGTLDRGGKRTVYTGSMLDPTNGKKV
                     AIRSVDTEVDADTLLFEMWMSGPDGKMAKSMEMTYTRKK"
     gene            complement(6726..8543)
                     /locus_tag="P5164_08250"
     CDS             complement(6726..8543)
                     /locus_tag="P5164_08250"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRY43922.1"
                     /translation="MSTMTARFAPRLSAVLPLLVLLAARPAGAVVPAAGPAATAAASG
                     PAVVVVPVANVLLRPEALAPVEDQAILGETLEATRAEGRYRYVRLRSGTRGWIEAEAI
                     RPGLPDPSAEALEVTSGLAHLYREPDFTSSAPLATVPLGARLGGLAALEKDGTRWREV
                     LLPDGRNAFASAEDLAPARAGGAPPVLDPTSWLAMARRFLGAPYTWGGTTPLGFDCSG
                     LVFRVLERHGILLMRNSSEMCFREPQLVPVPVEELAPGDLVFFGTEEKIDHVGFWAGN
                     DEVLQATTAGVPSTQETPWASPRLAPRFRYARRLAALPGAPPPSGLTPAKAEALGRTL
                     DALAAEGRATFGIVVKDLQTGGTAARNATTVMHAASTMKTAVLLEALRRVDAGSLSLA
                     TGVPVVDCFPSAVDGSPFRVPLDGENETRLAPFLGNAARLDFVAREMIVRSSNAATNL
                     MLGLCPPRDVQAFVDLLGAGGVKVRRMVEDEKAYQAGLSNETDAAGMAALMEAAVRSP
                     RLSADSRRLAFEILAAQEFNEQIPAGIPKQAGVAVAHKTGNISKVQHDAAVVRLPDGR
                     EYVLVLLATDFGANEEGRKRVIETTRKMSRAVWEAMIAP"
     gene            8553..9686
                     /locus_tag="P5164_08255"
     CDS             8553..9686
                     /locus_tag="P5164_08255"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HRY43923.1"
                     /translation="MSDPVSRRDLLSITGTLAASALLPGLAAAAPPAAAAPKGVTLSL
                     TPMLLKLRHTWTIARNSSSEKKNGLLSLAAAGITGYGETAANVRYGQSWESGEAAFAK
                     VKEACSGLSPWEHLAWLERAEAVCGGDSQVVAALDMALWDWKGKAVGQPVWKLLGVPA
                     GRMAQTTFSIGIDTAEVMKEKVKEAEPYPLLKVKVGLASDEENVSAIRSVTTKPIRVD
                     ANEGWKSAEEALAKITWLRTMGVEFVEQPLPVAKNAEMKAVKAGSVLPLVADESVLHV
                     KDVPSLVGLFDGVNAKLAKCGGITRAYELAALGRALGFRLMLGCMIESSLGIAAAVAV
                     APLYDWLDLDGNLLVASDPWKGLNLTDGRWELPEAPGLGVVPA"
     gene            complement(9721..>9929)
                     /locus_tag="P5164_08260"
     CDS             complement(9721..>9929)
                     /locus_tag="P5164_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43924.1"
                     /translation="TVVRLVGVLTAQLGLEAPWIVGHEHVAPMRKVDPGERFPWNEVM
                     RRGLELAERLRPVAPAAPDAAPAP"
BASE COUNT         1396 a         3504 c         3783 g         1246 t
ORIGIN      
        1 aggacgcggc cgagcgccgc ggcgaaatcc ttcatcggga cggggccggg ggcggtcccg
       61 ttcacgggcc cctcgtagcg cgggtcgtcc agggcggcga ggaggagggc gacgaggtcg
      121 tcgcggtgga tccagggcat ccactgcgcg ccaccgccga gcgggccgcc gaggccgagg
      181 cgaaagggaa agagcatttt cgcgagcgcc ccttcggccg ggtcgaggac gacgccggtc
      241 cggaggagga cgaggcggat gccctccggg accgcctccc gggcggcgcg ctcccaggcg
      301 agcgtcgtct cggcgaggaa gccgcttccc ggggggctct cctcggtcag ctcctcgtcg
      361 ccccggttcc cgtagaagcc gacggccgag gcgttgacga ggacgccggg agggctcttc
      421 gcggcgcgca cggccgcgcc ggcccgctcc gcggcctggg cccggctccc gacgatccgg
      481 gccttcgcgg acgcggtcca gcgctgggcg atcgtctcgc cggcgaggtt cacgacggcg
      541 ttggctccgt cgacggccgt cgcgaggccg tcccagccga cgccgcgggc gccgggcggg
      601 aggttggcgc gagacgggtc gcgggtcacg acgacggcct cgtctccccg gccggcgagc
      661 gccgcgacga gcgcgcgccc caggaaaccc gttccgcccg tgacgacgac cctcacggcc
      721 tcagctccgc tcgccctcgc cgcgccggat tctctcgatc gcccggtccc ggtcggcctc
      781 gatccgggtc cggtcgacgc ggtactcctc ctccgccttc tcccgttcga cggggtcgta
      841 gaccttcttg agggcgtcgc ggaagaggat ctcgcgctcg gcgagccggg aggaggcgac
      901 cgagcgggcc tcggcgatcg cggccttctg ggcgtcggtg agaggcttct cctcgacgcc
      961 ggattcgcgg tccgcggccc ggaggcgttc catggcgagc tcgaacgacg acttgggtgc
     1021 attcgacatg caggcatcgt aacgttccgg gcatgagcag gcacctcgcc cgccgaacgg
     1081 cgatcgtggg cctcgccctt ctggcggcgg cgccgagcct cggcgcggcc cggcagggcg
     1141 ccgaggccct cgtcccgatg tcggccgaag agctgatccg atccgtggcc cgcgcgcagc
     1201 ggcgggccga cctcgcggtg gcggcctcga cgttcgagca ggtggaggtg aagaccgact
     1261 tcgggcgcga cgggaggccg aaggcgatcc accgtcgacg gttccgttac accgccggcg
     1321 acgggagcgc cgtccggcgc gagctgctgg aggtggacgg gcgccccccc acggaggcgg
     1381 agatcgccga ggtggccgag gaggacgcga aggagcaccg gcgccgcgtc gaccggcggg
     1441 ccgctcaccg cgcctcccgg ccgcccgagg tgggcggctc cgaggaggac cccctcctcg
     1501 gcccgatccg cctctccgac ctgatctccc gcttctcgta cgggccggtc gaggaggtcc
     1561 tcgtcgacgg gcggccggcc tacgggctcg acttcgcacc gaggccgggg cgggtcgcga
     1621 cgcggaccct cgaccgggcg ctcgggtctc tcgccggccg cgtcctcgtc gacgcgaccg
     1681 acctccaggt cgtttcggtc gacgcccggc tcgtgacgcc gctccgggtg gggggaggcc
     1741 tcgcggcgag catccggagc gcgtcgatcg cgtaccgggc gcagcgcctc gcggacggcg
     1801 cctggctgcc gtgcctcgtc ggcatccgcc tccaggggcg gacgggggtc ctcttccggc
     1861 tcgacagcgc ctaccggttc gagttctccg actacgcccg cttcctcgtc gacgtcgaga
     1921 cggaggtcgg cagacccgga ggcccgtccg tacaatccgc gccgtgactc cctccaagcg
     1981 acgcctcgtc ctcggcctcg tcctcgtcgg cgccttcgcc ggcttcttcc tcgcgttccg
     2041 gacggcgctc gtcccggcga cccacgcgga ccacgaccac ggcatcctga acctcgacgc
     2101 gggcggctac ctcgaggtcc tgacgcgcgg ggggaagaag cggaacctcg tcggcgcccc
     2161 ggggcgggtc ctggcggtca cgttcgtcga cccggccgac ccggccgcgg ccgaggagct
     2221 gcgcggcctc ttcgcgttcc aggaggcgac gaaggggagc gaggaggccg aggtcgtcgt
     2281 cctcgtgaag gccgcctcct tcgccgagct cgacgcctgg ctcgcgagga gcgccctcgt
     2341 tcccccggtc cccgcgtcgc tgacggtcga cccggagggg aagacgacgg tcaagttcaa
     2401 caaccgccgt cccgtagaga cgatgttctt cggccccgac gggaagctcg cctcgcaggc
     2461 gcgcgggccg ctcgactggt cgctcgaggc gccccggcgg atcgccgccg cggcggccgg
     2521 cgagagcatc cactgaggtg aatcggggag gctcgccacg agcctccccg cggcgccttc
     2581 ggcgcctccc ctcgaaaggg gcaccggagc gaatcgggga ggctcgccgc gagcctcccc
     2641 gcggcgcgtc cgcgcctccc ctccctcggg ggcgcggagc gatcagcgga tcgtcgggac
     2701 gggggcggcg aggacgcccg cctgctccgc ctcgatctgc cggaggagcg gacgaagggg
     2761 tccgaggacg gtctcgaggg cggtgaagga gctctcgtcc tcctgctctc ccgagagcat
     2821 cgcggtgagc gcctcgaacg ggctcttctc cttcgggaag acggcgacct tcaccttctc
     2881 ggaggcgggg atcttcgccg cctccttcgc gaggcggagc gcgacggcgt agccgccgag
     2941 ctcgtcgacg agcccgagcg ccttcgcgtc ctcgccgctc cagacccgtc ccttcgcgat
     3001 ctcgaggacc ttctccttcg ggagcttccg cccgtcggcg accttcgagg tgaagtcgac
     3061 gtagacgcgg tcgagccacg cgttgaaccg ctcccactcg gcggggctga agctgcggga
     3121 ggcgttccag aggttcgcgt tcgcgccggc gtccacctgg tcgaacgtca ggccgacctt
     3181 ctcccacatc gcggtcgtca ccatcttccc gcccaggacg ccgatggagc cggtgatcgt
     3241 gccgggctgc gcgacgatct tcgaggccgg catggcgacg aagtagccgc cggaggcggc
     3301 gacctcgccc atcgagacga cgacgggctt gccggccttc tgggccagga cgacctcgcg
     3361 ccagatcgtg tcggaggcga cgtaggagcc gcccgggctc gagacgcgga agaggatcgc
     3421 cgcgacgtcg tcgtcggcga cggcgttccg gatcgccttc gcgacgcttt cggagcccat
     3481 cgtccgcccg cccgtgacgg ggttcgtccg gctcttcccg cggacgacgc cgccgacgcc
     3541 gtagacgagg gcgatcgtct tcctcccggc atcgtgcggc cggccttcct tcttcaggta
     3601 cgccgggagg ccgaagagct tcgccccggc gccccccttc ttcatcagct tctcgtggac
     3661 ctcgtcgcgg taggcgaggc cgtcgacgag cttcgcctcg agcgcctcgg ggccgaggaa
     3721 ggggccgcgg tcgacgaggg cgcgcacctc ctcctccgac atctgccgcc cctcggagat
     3781 ccccctcacc atctgcccga agaggtcgtc gacgagcgcc ttcgtcgcct cccggtgggc
     3841 gtcggtgaac ttcgtctccg tgtacatgtt catcgcgttc ttgaactcgt agcgctgccc
     3901 gaactcgggc ttgaccccga gcttgtcgag cgtgccccgg acgaacgggc tctcggcgac
     3961 gaggccgttg aggcagacgt cgccggaggg ctggagccag atctcgtcga acgcggtcgc
     4021 gaggtagtag gcgccgttcc ccggcgcgaa ctccccgaac gtctccgcgt aggcgaacgc
     4081 gagcttcttc ttcgcgcgga acgccttcac cgcgtcgcgc acctcctgga tctcggcgac
     4141 gccgatcggc gcggcgccga gccgcgcgac gaggccgacg acgtgcgggt cgtcccccgc
     4201 cttctcgagc gcgtcgagga cgtcgcggag cgtgagggga cgctcgccgc cgaagacggc
     4261 gaaggggtcg tcgggcgccg tctcggggag gccccgctcg aggtcgagct cgaggatcgc
     4321 ccggctcggg accgacggcg tcttccgccc ggcgaggaag acccctgcga ccaccaggcc
     4381 ggcgccgagg acgacgatcg cgagaacgag gacgaggaga cccttcccac ccttggccat
     4441 gcatccctct ccgtcgcccg gcttcggccg cggcggcgcg ggtcattatc ggcccggctt
     4501 catcgccgcc gtcgcccgcg ctccgagacc ggggccggct cgggcgctca ggcctccggg
     4561 gcggacggcg tcccgcgcac ctctcggcgg cggaaaaagg cgaggaggga ggctccggac
     4621 gcggcggcgc cggtgcccgc cgcgacgagg agcgctccgt gcgggccggg cgcgagcagg
     4681 gcgagaagga gccctcctgc cgaaagcgag gagatggctc cgagcgcggg gccggccacg
     4741 gccgcgagat agggcccgat gggccgcccg cggaacacga gccggtggag caggaacccc
     4801 cgcacgaggc cggcgccgag aagagccagg gccgcccctt ctcctccgag gcggccggcg
     4861 aggacgatga ggaggaggac gagaacggcc gcccccgcga tcgaggcgcg aaggacgtcc
     4921 ccgggtctgc ggtccgcgac gaggaggagg tcgaggaagt cgaagaagga ggggaggaag
     4981 ctcgcgagcg cgaggaggcg aagggcgccc gccgcggggg cgtagtcgtc gccgaaaagg
     5041 aggacgagga gggggcccgg cgacaggagg cagacggaga ggacgggcca gacgagcgcg
     5101 aacgcgaggt ggcgcgtcgc gacgtggagg cgggcgaggc gggcctcgtc tccctccgcg
     5161 acggccgagg cggccgacgg gaggtagccg tgggaggacg cctggcggac caggtcgagg
     5221 acgcgcgtga ggccgcgcgc gacgccgtag aggccgacgg cggtcgccgg agcgagggcg
     5281 ccgaggacga cgacgtcgag ccagagcgcc gtctgcgtca gcacctcgtt cgccgcgaag
     5341 gggcggaggt cgggaagggc gacgggaccg cccgcgtgcg cggggggagc cgaaagccag
     5401 cccttccgcg ccacgtaggc gacgaagaga agctcggccg cgatcgatcc ggcggcgaac
     5461 ccggcggcga cggcgaagga gctcgccgtg cccgcgagga gagcggtgcc gacgcccgcg
     5521 acccggagaa ggccgccccc cgcgtccctc acgagcgcgc ggccgagcga gtcgccgaac
     5581 ccccgcgaga cgccgagcgc ggccgcacct gcagcgagcc cgagcgccac gggcgccatc
     5641 gggaggagga cgtggtcgat tcccggctgg tggaggagcc gcgccagcgg tccggcgagg
     5701 agggcgagga ggagagtggc ggcgaggccg gaagccgaga cgaggaggag agccttcccc
     5761 ccgatccgcc gcgcggcggt ctcctcgccg ccggcgcgaa gctcggccag cctctgcgcc
     5821 atcgcggcgt tcgtcccgag gccggcgatc gaaccgacgg gggtgacgac cgcgatcccg
     5881 aggaggacga ggccgagggc gtcgggctcg agcgcccggg ccatcaggac gcgaacgacg
     5941 aggctcccca cgagcgcgac gccgcttccc gcgaggacgg agagggtcga gacgaggatg
     6001 gcgcggcgtg cgccggggcc ggacggcacc gggggagcct aatcccatcg ggacggacgt
     6061 gcgagactgc gcgaaacctt ccgagcctcg tgaccagaga ggagaccgca tgagcctcgt
     6121 tcgctggatg acccttcccg cactcgtcct cgcgttccac gcctccgcgc aggagaagcc
     6181 gacgtcggcc cccgccgccg ccgcggagga ggcgatgaag gcctgggcgg cctacgcgac
     6241 gccgggagag gcgcacgcgc gcctcgcgaa gctcgagggg acctgggacg tgaagacgaa
     6301 gtcgtggatg gacccgagcc agcccccgga ggagacgacg gggagctgcg acttccggat
     6361 ggtcctcggc ggccgctacc tcgagcagcg cttccagggg acggcgatgg gccagccctt
     6421 ctcgggcatc ggctacaccg gcttcgacaa cgtgaagaag aagtacgaag cgtactggat
     6481 cgactcgatg gggaccggga tgatggtgat gtccgggacg ctcgacaggg gcgggaagag
     6541 gaccgtctac acgggctcga tgctcgaccc gacgaacggc aagaaggtcg cgatccggtc
     6601 ggtcgacacg gaggtcgacg ccgacacgct cctcttcgag atgtggatgt cgggcccgga
     6661 cgggaagatg gcgaagtcga tggagatgac ctacacgcgg aagaagtagg cggagccggg
     6721 cggactcacg gcgcgatcat cgcctcccag acggcgcggg acatcttccg cgtcgtctcg
     6781 atcaccctct tccggccctc ctcgttcgcg ccgaagtcgg tcgcgaggag gacgaggacg
     6841 tactcgcgcc cgtcggggag cctcacgacg gccgcgtcgt gctggacctt cgagatgttc
     6901 ccggtcttgt gcgcgacggc gacgccggcc tgcttcggga tcccggccgg gatctgctcg
     6961 ttgaactcct gcgccgcgag gatctcgaac gcgaggcggc gcgaatcggc ggaaaggcgg
     7021 ggcgagcgga ccgccgcctc catcagggcg gccatgcccg cggcgtcggt ctcgttcgag
     7081 aggccggcct ggtaggcctt ctcgtcctcg accatccggc gcaccttcac gccccccgcg
     7141 ccgaggaggt cgacgaacgc ctgcacgtcc ctcggcgggc agaggccgag catcaggttc
     7201 gtggcggcgt tcgaggagcg gacgatcatc tcccgcgcga cgaagtcgag gcgggccgcg
     7261 ttcccgagga agggggcgag gcgcgtctcg ttctccccgt cgagcggcac gcggaacggg
     7321 ctcccgtcga cggcgctcgg aaagcagtcg acgaccggaa cgcccgtcgc cagggagagg
     7381 ctccccgcgt cgacgcgccg aagcgcctcg aggaggaccg cggtcttcat cgtcgaggcg
     7441 gcgtgcatca cggtcgtcgc gttgcgcgcg gccgtcccgc cggtctgcag gtccttcacg
     7501 acgatcccga aggtcgcccg cccctcggcg gcgagggcgt cgagggtccg gccgagggcc
     7561 tcggccttcg cgggggtgag gccggagggg ggcggggctc cggggagcgc cgcgaggcgg
     7621 cgcgcgtagc ggaaacgcgg cgcgagccgc gggctcgccc agggcgtctc ctgcgtcgac
     7681 gggactccgg ccgtcgtcgc ctggaggacc tcgtcgttcc cggcccagaa gccgacgtgg
     7741 tcgatcttct cctccgtccc gaagaagacg aggtcccccg gggcgagctc ctcgaccggg
     7801 accgggacga gctgcggctc gcggaagcac atctccgagc tgttcctcat gaggaggatg
     7861 ccgtgccgct cgaggacccg gaagacgagg cccgagcagt cgaatccgag cggcgtcgtg
     7921 cccccccatg tgtacggcgc gccgaggaag cggcgcgcca tggcgagcca ggaggtgggg
     7981 tcgaggacgg ggggcgcgcc gcccgcccgg gcgggggcga ggtcctccgc gctcgcgaag
     8041 gcgttccggc cgtcggggag gaggacctca cgccagcggg tgccgtcctt ctccagcgcc
     8101 gcgaggcctc cgagacgggc gccgagcggc acggtcgcga gcggagcgga ggacgtgaag
     8161 tcgggctcgc ggtagaggtg cgcgaggccc gaggtcacct cgagcgcctc ggccgacggg
     8221 tcggggaggc cgggccggat cgcctccgcc tcgatccagc cgcgggtgcc ggagcgaagg
     8281 cgaacgtagc ggtagcgccc ctcggccctc gtcgcctcga gcgtctcccc gaggatcgcc
     8341 tggtcctcca cgggggcgag cgcctccggc cgcaggagga cgtttgcgac cgggacgacg
     8401 acgacggccg gcccggaggc cgccgcggtc gccgccgggc cggcggccgg gacgaccgct
     8461 ccggccgggc gcgccgcgag gaggacgaga aggggcagga ccgcggaaag acggggcgcg
     8521 aaacgggcgg tcatcgtgct cataatcgtc gcatgtccga ccccgtctcg cgccgcgacc
     8581 tcctctccat caccggcacg ctcgccgcgt ccgcgctcct ccccggcctc gccgccgccg
     8641 cgccccccgc ggccgcggct ccgaagggcg tgacgctctc cctgacgccg atgcttctga
     8701 agctgcggca cacctggacg atcgcgcgca actcctcgag cgagaagaag aacggcctcc
     8761 tctcgctcgc cgcggccggc atcaccggct acggcgagac cgcggcgaac gtccgctacg
     8821 gccagagctg ggagagcggg gaggcggcct tcgcgaaggt gaaggaggcg tgctcggggc
     8881 tttcgccgtg ggagcacctc gcctggctgg aacgggcgga ggccgtctgc ggcggcgact
     8941 cgcaggtcgt cgcggcgctc gacatggcgc tctgggactg gaaggggaag gcggtcgggc
     9001 agccggtctg gaagctcctc ggcgtcccgg ccgggcggat ggcgcagacg acgttctcga
     9061 tcgggatcga cacagccgag gtgatgaagg agaaggtgaa ggaggccgag ccgtaccctc
     9121 tcctgaaggt gaaggtcggc ctcgcgagcg acgaggagaa cgtctcggcg atccgatccg
     9181 tcacgacgaa gccgatccgc gtcgacgcga acgaggggtg gaagagcgcg gaggaggctc
     9241 tcgcgaagat cacctggctg aggacgatgg gggtcgagtt cgtcgagcag ccgctccccg
     9301 tcgcgaagaa cgccgagatg aaggcggtga aggcggggtc ggtcctgccg ctcgtcgcgg
     9361 acgagtcggt cctccacgtg aaggacgtcc cgtccctcgt gggcctgttc gacggagtga
     9421 acgcgaagct cgccaagtgc ggcggcatca cccgcgcgta cgagctcgcg gcgctcgggc
     9481 gggcgctcgg gttcaggctg atgctcggct gcatgatcga gtcgtcgctc gggatcgcgg
     9541 cggccgtcgc ggtcgcgccg ctctacgact ggctcgacct cgacgggaac ctcctcgtcg
     9601 ccagcgaccc gtggaagggg ctgaacctga cggacggacg ctgggagctg ccggaggccc
     9661 ccggcctcgg cgtcgtcccg gcctgaggag cgggcgggag cgtttctccc gcctcgccgg
     9721 ctacggcgct ggagccgcgt ccggggcggc gggtgcgacg gggcgaagcc tctcggcgag
     9781 ctcgaggccc cgccgcatca cctcgttcca ggggaagcgc tcgcccgggt cgaccttcct
     9841 catgggagcg acgtgctcgt gcccgacgat ccacggcgcc tcgaggccga gctgggccgt
     9901 cagaacgccg acgagcctca cgacggtct
//
LOCUS       DAOTEZ010000125         9925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4814_length_9925_cov_4.887741, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000125 DAOTEZ010000000
VERSION     DAOTEZ010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9925
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4814_length_9925_cov_4.887741"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="P5164_08265"
     CDS             complement(<1..361)
                     /locus_tag="P5164_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43925.1"
                     /translation="MSRTVTFAKAVAFAVAALAAAPAPADTIATTKAFLPAVNIDGAT
                     GTFLTDVWIFNPEPSLENEVTLYFTPAGVDGSNLQGLRINPPLAPRESVALKDILKNY
                     LGKTKTFGLLEVRGSYPI"
     gene            complement(492..734)
                     /locus_tag="P5164_08270"
     CDS             complement(492..734)
                     /locus_tag="P5164_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43926.1"
                     /translation="MKKILSVVALIAVSASLVACGGEKKEAAPAEAPAVVEEPAPATE
                     AAPATTEAAPATTEAAPATTEAAPAPTTEAAPAPAK"
     gene            1091..2392
                     /gene="ffh"
                     /locus_tag="P5164_08275"
     CDS             1091..2392
                     /gene="ffh"
                     /locus_tag="P5164_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRY43927.1"
                     /translation="MFDSLSDRLQRVMKGLRGEGHLTEFHVDEALKEIRTGLLEADVH
                     LDVVKGFAQRVREKAVGQEVLTAFAPAQQVLKIVQAELVALLGGTTANLVLKRRPSVI
                     LLVGLQGSGKTTTAAKLALHLKKKLGKQVLLVPADVYRPAATEQLRTLGKENGLPCFD
                     PKGETDAVARVKAAIKEAKLFGWDVAIVDTAGRLHLDDALMDEVARIREVCDPDEVLF
                     VADAMTGQDAVTSARAFSDRLSLTGVILTKLDGDARGGAALSLSTVVGRPIKFAGIGE
                     KTVDLEPFHPDRAASRILGMGDVMTLIEKVSSEADQKAAQKAVERLRKNEFTLDDLRD
                     HFRQLKRLGPLSTLVGHLPKVGPFKQLRDLQIEDDATKHLEAMIDSMTAEERNDPKLL
                     NGSRKLRVARGSGTSVQDINQLLKQYLEMKKMMKGAARMRF"
     gene            2430..2666
                     /gene="rpsP"
                     /locus_tag="P5164_08280"
     CDS             2430..2666
                     /gene="rpsP"
                     /locus_tag="P5164_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRY43928.1"
                     /translation="MLKIRLRRTGGTNAPAYRIVVSDSRRTPTAMVIEELGYYDPTKN
                     PPLLDLDVDKANAWIAKGATASGTVGKLLARPKA"
     gene            2684..3205
                     /gene="rimM"
                     /locus_tag="P5164_08285"
     CDS             2684..3205
                     /gene="rimM"
                     /locus_tag="P5164_08285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRY43929.1"
                     /translation="MAAEDLVVVGRLARPHGLRGEMSVEVLSDFPERFAPGLVLVAAA
                     PSGESRGELVVSTVRPAGERLLIGFQGIETRTAAEALRGLDVAVPAGREMPRPEGFVY
                     HFDLEGCRAVDRTGRELGVVTDLQEVGGRDLLSIRTPAGERDVPFVEPIVVSVDVARK
                     LVVLDPPPGLLDE"
     gene            3202..3954
                     /gene="trmD"
                     /locus_tag="P5164_08290"
     CDS             3202..3954
                     /gene="trmD"
                     /locus_tag="P5164_08290"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRY43930.1"
                     /translation="MRFDLVTIFPGYFASPLGEALVGKAIESGLLEVRVVDLRPFSED
                     PHRKVDDEAFGGGPGMVLAAPPLVAALEALRAEEGAAAPHVVVLSPRGRRLDAALARE
                     LALRPRLVLVCGRYEGIDERVREAGLVDEEVSLGDFVLSGGEVAALALLEAVSRFVPG
                     VVGEEDSVRLDSFEDGLLDHPHYTRPREFRGLAVPEPLLSGHHERIRRWRREQQFEQT
                     ARHRPDLLDRFAPRTPEDRELLDRVARRGRTP"
     gene            4036..4383
                     /gene="rplS"
                     /locus_tag="P5164_08295"
     CDS             4036..4383
                     /gene="rplS"
                     /locus_tag="P5164_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRY43931.1"
                     /translation="MDKLLEAERQALRGDIPPFAPGDTVKVHVKVREGEKERIQVFNG
                     LVIARKGGGVRETFTVRKVSQGIGVERTFPVHSPVLEKIEVERHGVVRRAKLYYLRKL
                     RGKAARIAEKRDA"
     gene            4445..5179
                     /locus_tag="P5164_08300"
     CDS             4445..5179
                     /locus_tag="P5164_08300"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRY43932.1"
                     /translation="MDRRSGSPPSSPRVASRERGDGRSSLLSDEDARVRALLAVEDAA
                     RASGARRVAGVDEAGRGALAGPVHAAAVVLPAGLLLRGLDDSKALSPSVREALAPRIQ
                     ARAEAWGVGAASAAEIDELGIAAASFLAMRRALARLAGGPPDLVLVDGFPIPGLGLPQ
                     RAVVKGDATVACISAASVLAKTDRDAELRALDARSPWYGFAAHKGYGSAAHLEALRRH
                     GPSPDHRLTFAGVLSRARDASTVAVA"
     gene            5201..9319
                     /locus_tag="P5164_08305"
     CDS             5201..9319
                     /locus_tag="P5164_08305"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY43933.1"
                     /translation="MASKRESLIASAEKSLLKGKVDAALKDYLKVLEETPNDINVLNK
                     VGDLYVRLGRNDESIPFFLRIAEHYARDGFYVRGIAMYKKINKLDPARLDIYEKLAEL
                     YVKQQLWMEAKSNYQVLADYLLKQDNLQGAVGIYQKMVAIEPQNLQLHVRLADLFTQA
                     KRLPEALQEYAVVAAALSERGANEESIRVYEKALKLAPDNVEILRTLVPLLLSINSVE
                     QARTVLRKGLEASPRSVPLFLLAAEAALAANDMAEARSYSDKARAVDPENEEVLSAAV
                     RIQLKGRRPDLAWGAALPLADASVRRGETKKALALLVPIARAAPDNEEIVRKIVDLAA
                     GSGDETTALPFRSALAELYRKKGMLVEAADLLRICARVQPDNTEFRSRLSQLEAKAGI
                     LPPSPPRVPPPTLDRNLEVTLSGFDLPERRPGAPAEIPQSPREVLVPAPDSASEFEFD
                     LDDADLAEEEAPAAAEEAPPTPPRGLQAPSRYHGVEPEAAGDEPAWGTLSAAEALEAF
                     EERQAEAARTAAGGAADGAIEELLPIAQMSPATPVSGIPAPRGFEVPLAAPDGFGPST
                     IDAEPDFPPPSFGDALSSEPAWPMPDAGPAPSGLEGGGPASWGPVSGAIAADAAVEDA
                     LVEADVFRRYGLLEKALDQLLPWIERAPGNLKVRERLFEITLEQGNREAARGHAIVLA
                     EAYEAVGREDRIRGLEGLLGESLRAAAEEPPAVVEAPAEARSGADGSLPEEEIEPASA
                     GEAAAPSPAASLPVDLPGGLAMPQGLGASEEPLTEILSGPIALPVEAETLEGAESDVP
                     RIAPVEPAAGGPPAPERPSLEEEIAAEAGPSLEPGLPVSGALVVEEGLETDLAAAVSL
                     PLPAEPLPAAEEIPRGDAGGAPAEAVDVPVEAPLAAPSSEPLTAASEAESASDAPAGR
                     PVVSTPRTKLSAEDLLGLERPVRPAAPKKVRTVRPEDVELDLLGRKEAPRTKAAKPSR
                     PDVVLPDDLLKSLGKRPHAPAALEHEIAERVPSPASGAGADLEAAAVEAPLVVEPAAE
                     EPPAVEPLAEEPPAVEAPAEEPILAAEADALAAEAVPTDDAVAREEPETEPAPPPAGP
                     SEEELSELDFCLDQGMVVDAAERLQGLEGRFPGSPDLAVRRVRLEGGRGGTEASRASL
                     HDLLSDDLDSVLDAELGRSLTEDMVRESNQPSGPIPVVPEGAPALDESGLFSDEQEFF
                     NFADELHTELRKEGETAPAEEGREVSLEEIFRDFKKGVEQQLSPEDYETHYNLGIAYK
                     EMGLTDEAIGEFQLAAKDPLHAVECCAMLGLCFLEKGLPQLAVKWYRKGLDTVGIKDD
                     DRLGLQYALAGVLEQIGDAEGAYRTYLEIFSSNAAYRDVPARIKELRPIIAP"
     gene            9414..>9925
                     /locus_tag="P5164_08310"
     CDS             9414..>9925
                     /locus_tag="P5164_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY43934.1"
                     /translation="MTRDGAAGPGDAPLLTVRSLAVDFGSTRVVDGVSFHVSRGEIVA
                     LVGESGSGKTVTALSLLDLVPMPGRRTAGDVVFEGRDVTGLSPSELRRLRGGGIGFVF
                     QEPGEALDPVRTIGSELVAVLRRHRGLSRDEARREALSWLSRVALPDPEARMRDLPHR
                     MSGGMRQRAM"
BASE COUNT         1424 a         3593 c         3529 g         1379 t
ORIGIN      
        1 tgatcgggta gctgccccgc acctcgagga gcccgaacgt cttcgtcttg ccgaggtagt
       61 tcttgaggat gtccttcagg gcgaccgact cgcgcggggc gaggggggga ttgatccgga
      121 ggccctggag gttcgagccg tcgacgccgg ccggtgtgaa gtagagcgtg acctcgttct
      181 cgagcgacgg ctccgggttg aagatccaga cgtcggtgag gaacgttccc gtcgcgccgt
      241 cgatgttcac ggcgggaagg aaggccttcg tcgtggcgat cgtgtccgcc ggcgcgggcg
      301 cggcagcgag tgcggcgacg gcgaaggcga cggcttttgc gaacgtgacg gtgcggctca
      361 tgctgacctc cggcctctcg gccgatccgg atcgtgcaag cgtcctgcca gcggagcgtc
      421 tcccggcgga gacgaaaaag agaaacgccc cgctcgcgcg gggcgcttcc gaaggacgaa
      481 tcgagctcgg cttacttggc cggggccggg gcggcctcgg tcgtcggggc cggggcggcc
      541 tcggtcgtgg ccggggcggc ctcggtcgtg gcgggggcgg cctcggtcgt ggcgggggcg
      601 gcctcggtgg ccggggcggg ctcctcgacg acggcggggg cctcggcggg ggcggcttcc
      661 ttcttctcgc cgccgcaggc gacgagggag gcggagacgg cgatgagggc gacgacggaa
      721 aggatcttct tcatggtgac tccttctttc ttttccgggg cgattcttcg ctgcgattca
      781 gcgacgtcgg ttcggtttgc gttcttcgct tgtgggcggc gttccgagtc tcgaaccggc
      841 ctggcggccg ggacggcgtt tcgtggtgcg cggtgtcgac cccgcttcac acaccaaaac
      901 cgtcgacgag tttcctcgcc cgagcttcga ggagatgcta gaggaatccg cgcgcctgtc
      961 aatagaaaaa aacgagctga gtcccggcgc caggacccgc cagaggtcgg tcggacgaaa
     1021 ccgtccgcct ggaaaggctc cgccgtttga ccaccccggc agcggcacgt agactccgcg
     1081 tcttcgggcg atgttcgact cactgtccga ccgtctgcaa cgcgtgatga aggggctccg
     1141 gggtgagggt cacctgacgg agttccacgt cgacgaagct ctcaaggaga tccggaccgg
     1201 gctcctcgag gcggacgtcc acctggacgt cgtcaagggc ttcgcccagc gtgtccggga
     1261 gaaggccgtc ggccaggagg ttctgaccgc cttcgcgccg gcgcagcagg tcctgaagat
     1321 cgtccaggcc gagctcgtcg cgctcctcgg cgggactacg gcgaacctcg tcctgaagag
     1381 gcgcccctcg gtcatcctcc tcgtcggcct ccagggctcc ggcaagacga cgaccgccgc
     1441 gaagctcgcc ctccacctca agaagaagct cggcaagcag gtcctcctcg tcccggccga
     1501 cgtctaccgc cccgccgcga cggagcagct ccggaccctc gggaaggaga acggcctgcc
     1561 gtgcttcgac ccgaaggggg agaccgacgc ggtggctcga gtgaaggcgg cgatcaagga
     1621 ggcgaagctc ttcggctggg acgtcgccat cgtcgacacc gccggccgcc tccacctcga
     1681 cgacgccctc atggacgagg tcgcccggat ccgcgaggtc tgcgaccccg acgaggtcct
     1741 cttcgtcgcc gacgcgatga ccggccagga cgccgtcacg tcggcccgcg cgttctccga
     1801 ccggctctct ctcaccgggg tcatcctgac gaagctcgac ggcgacgccc gcggcggcgc
     1861 cgcgctctcc ctctcgaccg tcgtcggcag gccgatcaag ttcgccggca tcggcgagaa
     1921 gaccgtcgac ctcgagccgt tccaccccga ccgtgccgcg tcgcggatcc tcgggatggg
     1981 cgacgtgatg acgctcatcg agaaggtctc ttcggaggcc gaccagaagg cggcccagaa
     2041 ggccgtggaa cgcctccgga agaacgagtt cacgctcgac gacctgaggg accacttccg
     2101 tcagctcaag cgcctcggcc cgctctcgac gctcgtcggg cacctcccga aggtcgggcc
     2161 gttcaagcag ctccgcgacc ttcagatcga ggacgacgcg acgaagcacc tcgaggcgat
     2221 gatcgactcg atgaccgccg aggagcggaa cgacccgaag ctgctgaacg gaagccggaa
     2281 gctccgcgtc gcgcgcggct cgggcacctc tgtccaggac atcaaccagc tgctcaagca
     2341 gtacctcgag atgaagaaaa tgatgaaggg cgccgcccgg atgcgcttct gatccggatg
     2401 gccctggaca ccgcgccgaa aggaaaccga tgctgaagat ccgactccgc cgcacgggcg
     2461 gcacgaacgc cccggcgtac cgaatcgtcg tctccgacag ccgccgcacc ccgaccgcca
     2521 tggtcatcga ggagctcggc tactacgacc cgacgaagaa cccgccgctc ctcgacctcg
     2581 acgtcgacaa ggcgaacgcc tggatcgcca agggcgcgac cgcgtccgga acggtcggca
     2641 agctcctcgc ccggccgaag gcctgacggg cgccgggccg gccgtggctg cggaggacct
     2701 cgtcgtcgtc ggacgcctcg cgcggccgca cggcctgcgc ggcgagatga gcgtcgaggt
     2761 cctctccgac ttccccgaac ggttcgcacc ggggctcgtc ctggtcgcgg cagcgccttc
     2821 cggggagtcc cgcggcgagc tcgtcgtctc gacggtgcgt ccggcgggag agcgcctgct
     2881 gatcgggttc caggggatcg agacccggac cgcggccgag gccctccggg gcctcgacgt
     2941 cgcggtgccg gccggccgcg agatgccgcg ccccgaggga ttcgtctacc acttcgatct
     3001 cgaagggtgc cgcgccgtcg accgcacggg gcgcgagctc ggcgtcgtga ccgatctcca
     3061 ggaggtcggg ggtcgggacc tcctctcgat ccgcacgccg gccggcgagc gggacgtccc
     3121 gttcgtcgag ccgatcgtcg tctcagtcga cgtggcgcga aagctcgtgg tcctcgaccc
     3181 tcctccgggg ctcctcgacg aatgaggttc gacctcgtca cgatcttccc cgggtacttc
     3241 gcctccccgc tcggggaggc tctcgtcggc aaggcgatcg aaagcggcct cctcgaggtg
     3301 cgggtcgtcg accttcgccc cttcagcgag gacccgcacc ggaaggtcga cgacgaagcg
     3361 ttcggcgggg ggcccgggat ggtcctcgcg gcgcccccgc tcgtcgccgc cctcgaggcg
     3421 ctccgggccg aggagggcgc ggccgcgcct cacgtcgtcg tcctctcgcc gcggggtcgg
     3481 cggctcgacg cggcgctggc ccgcgagctc gcgctgcgcc cgcgactggt gctcgtctgc
     3541 ggccggtacg aggggatcga cgagcgggtg cgcgaggccg gtctcgtcga cgaggaggtc
     3601 tccctcggcg acttcgtcct ctcgggcggc gaggtcgcgg cgctggcgct cctcgaggcg
     3661 gtctcgcggt tcgtgccagg ggtcgtgggg gaggaggaca gcgtccggct cgacagcttc
     3721 gaggacgggc tgctcgacca tcctcactac acccgcccgc gcgagttccg ggggctcgcc
     3781 gtccccgagc cgctcctttc cggccatcac gagaggatcc ggcggtggcg ccgggagcag
     3841 cagttcgagc agacggcgcg ccatcgtccg gacctcctcg accgcttcgc cccccggacc
     3901 ccggaggacc gcgagctcct cgaccgggtt gcccgaagag gccggactcc gtagaatccg
     3961 cgttctggcc atccggacgg ggacaggtgt cctcgagtgc cggggtggcc aagacgtctt
     4021 cggaggttcc tcgaaatgga caagctgctc gaggcggaac gccaggctct gcgcggggac
     4081 attccgccat tcgcccccgg cgacaccgtg aaggtgcacg tcaaggtgcg cgagggggag
     4141 aaggagcgga ttcaggtctt caacggtctc gtcatcgccc gcaagggcgg cggggtccgg
     4201 gagaccttca cggtgcgcaa ggtctcccag gggatcggag tggagcggac gttccccgtc
     4261 cactctccgg tcctggagaa gatcgaggtg gagcgccacg gcgtcgtccg ccgcgccaag
     4321 ctctactacc tccggaagct ccgcggcaag gccgcccgta tcgccgagaa gcgggacgcc
     4381 tgaaccttcg ggggcgtgac gcccccgggc ggtgcgccgg cgcgcccgcc ggacgaggag
     4441 agggatggac cgtcgttcgg gctctccgcc ttcgtcgccc cgcgtcgcct cgcgcgagcg
     4501 gggcgacggc cgttcctccc tcctctcgga cgaggatgcc cgggtccggg ccctcctcgc
     4561 ggtcgaggac gcggcgcgcg cgagcggagc gcggagggtc gccggagtcg acgaggcggg
     4621 acggggcgca ctggccggcc cggtccacgc ggccgcggtc gtcctccccg ccggacttct
     4681 cctgcgcggc ctcgacgatt cgaaggccct ctctccctcc gtccgcgagg cgctcgcccc
     4741 gcggatccag gcccgcgcgg aggcgtgggg cgtcggcgcg gcctcggcgg ccgagatcga
     4801 tgagctcggc atcgccgccg cctcgttcct cgcgatgcgg cgagcgctcg cccggctcgc
     4861 cggagggcct ccggacctcg tcctggtcga cggcttcccg atccccgggc tcggcctccc
     4921 gcagcgagcg gtcgtgaaag gagacgccac ggtcgcctgc atctccgccg cctcggtcct
     4981 cgcgaagacg gaccgggacg ccgagctgcg agccctcgac gcgcgaagcc cgtggtacgg
     5041 gttcgccgcg cacaagggct acggaagcgc cgcgcacctc gaggcgctcc gccgccacgg
     5101 accgtcgccg gaccaccggc tcacgttcgc cggcgtcctt tcgcgagccc gcgacgcatc
     5161 caccgttgcg gtggcctgag gaatcgggcg aagatcagcc gtggccagca agcgcgagtc
     5221 gctgatcgct tccgccgaga agtccctgct caaggggaag gtcgacgccg cgctcaagga
     5281 ctacctgaag gtcctcgaag agaccccgaa cgacatcaac gtcctgaaca aggtcggcga
     5341 cctctacgtc cggctcggac ggaacgacga gtcgatcccc ttcttcctgc gcatcgcgga
     5401 gcactacgcc cgggacggct tctacgtccg cggcatcgcg atgtacaaga agatcaacaa
     5461 gctcgacccg gcccgcctcg acatctacga gaagctcgcg gagctctacg tcaagcagca
     5521 gctctggatg gaggcgaaga gcaactacca ggtcctcgcc gactacctcc tcaagcagga
     5581 caacctccag ggagccgtcg ggatctacca gaagatggtc gcgatcgagc cgcagaacct
     5641 gcagctccac gtccgcctcg ccgacctctt cacccaggcc aagcggctcc ccgaggcgct
     5701 tcaggagtac gcggtcgtcg ccgcggctct ctccgagcgc ggcgccaacg aggagtcgat
     5761 ccgcgtctac gagaaggcgc tgaagctcgc gccggacaac gtcgagatcc tgcggacgct
     5821 cgtccccctg ctcctctcga tcaactccgt cgagcaggcg cggaccgtgc tccgcaaggg
     5881 gctcgaggca agcccgcgct ccgtcccgct cttcctcctc gccgccgagg cggctctcgc
     5941 cgcgaacgac atggccgagg cgcgcagcta ttcggacaag gcgcgcgcgg tcgaccccga
     6001 gaacgaggag gtcctctcgg ccgcggtccg gatccagctg aagggtcgcc ggcccgacct
     6061 cgcgtggggc gcggcgctgc ccctggcgga cgcgtcggtc cggcgcggcg agacgaagaa
     6121 ggcgctcgcc ctcctcgtcc cgatcgcgcg ggccgcgccg gacaacgagg agatcgtccg
     6181 gaagatcgtc gacctcgccg ccggctcggg cgacgagacg acggccctgc cgttccgttc
     6241 cgcgctcgcc gagctgtatc ggaagaaggg gatgctcgtc gaggccgcgg acctcctgcg
     6301 catctgcgcc cgggtgcagc ccgacaacac cgagttccgg tcccgcctct cccagctcga
     6361 ggcgaaggcc ggcatcctcc cgccctcgcc tccccgcgtc cccccgccga cgctggaccg
     6421 gaacctcgag gtgacgctct cgggattcga cctcccggaa cggcgcccgg gcgcgccggc
     6481 ggagatcccg cagtcgccgc gggaggtgct cgtccccgcc cccgactccg cgtccgagtt
     6541 cgagttcgac ctcgacgacg ccgacctggc cgaggaggaa gccccggccg cggccgaaga
     6601 agcgcctccg accccgccgc gggggctcca ggcaccatcg cgttaccacg gcgtcgagcc
     6661 cgaagcggcg ggcgacgagc cggcctgggg gacgctctcc gccgcggagg cgctcgaggc
     6721 tttcgaggag cggcaggccg aggcggcgcg cacggccgcc ggcggtgcag cggacggggc
     6781 gatcgaagag ctcctcccga tcgcgcagat gtcgcccgcc acgcccgtct ccgggattcc
     6841 cgcgcctcgc gggttcgagg tcccgctcgc tgcgccggac gggttcggac cgtccaccat
     6901 cgacgccgag cccgacttcc cgcccccgtc gttcggggac gcgctttcga gcgagcccgc
     6961 gtggccgatg cccgacgcgg gaccggcgcc gtccggcctc gaagggggag gccccgcgtc
     7021 ctggggaccg gtttccggag cgatcgcggc ggacgctgcg gtcgaggacg cgctcgtcga
     7081 ggcggacgtc ttccgtcgct acggactcct cgagaaggct ctcgaccagc tccttccgtg
     7141 gatcgagagg gcgccgggga acctgaaggt ccgcgagcgg ctcttcgaga tcacgctcga
     7201 gcagggaaac cgggaggccg cgaggggcca cgccatcgtc ctcgcggaag cctacgaggc
     7261 cgtcggccgc gaagaccgga tccgagggct ggaggggctg ctgggagagt cgctgcgcgc
     7321 cgcggcggag gagccgcccg ccgtcgtcga ggcgccggcc gaagcgcgat ccggcgcgga
     7381 cgggagcttg ccggaggaag agatcgagcc ggcttccgcc ggcgaggccg ccgccccttc
     7441 gcccgcggcc agccttccgg tcgaccttcc cggcgggctc gcgatgccgc agggcctcgg
     7501 ggcgagcgag gagccgctga cggagatcct ctcgggcccg atcgcccttc ccgtggaggc
     7561 cgagaccctc gaaggcgcgg agtccgacgt cccccgaatc gctcccgtcg agccggcagc
     7621 gggtggaccg cccgcgccgg agcgaccgtc gctcgaggag gagattgccg ccgaggcggg
     7681 accctcgctc gagcccgggc ttcccgtgtc cggggcgctc gtcgtcgagg aaggcctcga
     7741 gaccgacctg gccgccgcgg tatccctgcc gctgccggcc gagccgctcc cggcggcgga
     7801 ggagattccg cggggagacg cgggcggcgc ccccgcggaa gcggtcgacg tccccgtcga
     7861 agcgccgctc gcggccccct cctcggagcc gctcaccgcc gcttcggaag ccgagagcgc
     7921 ctccgacgcc ccggccgggc gtcccgtcgt ctccacgccg cggacgaagc tctccgcgga
     7981 ggacctcctc ggtctcgaaa ggcctgtccg gccggcggcg ccgaagaagg tccgcacggt
     8041 ccgacccgag gacgtcgagc tggacctgct gggcaggaag gaagcgccga ggacgaaggc
     8101 cgcgaagccg agccggcccg acgtcgtcct ccccgacgac ctgctgaagt cgcttggaaa
     8161 gcggccgcac gcacctgcgg ccctcgagca tgagatcgcc gagagggttc cgtcgcccgc
     8221 ctccggggcg ggcgcggacc tggaggccgc ggccgtcgag gcgccgctcg tcgtcgagcc
     8281 ggccgcggag gagccgccgg ccgtcgagcc gctcgcggaa gagccgccgg ccgtcgaggc
     8341 gcccgcagag gagccgatcc tcgcggccga agcggacgcg ctcgcggccg aggcggttcc
     8401 gaccgacgac gcggtcgcgc gcgaggagcc cgagaccgag cccgcaccgc ccccggcagg
     8461 tccttcggag gaagagcttt ccgagctcga cttctgtctc gaccagggga tggtcgtgga
     8521 tgccgcggaa cggctccagg gcctggaggg ccgattcccg gggagcccgg acctcgccgt
     8581 gcgtcgcgtg cgtctcgaag gaggccgcgg tggcacggag gcgtctcggg cctcccttca
     8641 cgacctcctc tccgacgacc tcgactcggt cctcgacgcc gagctgggcc gttcgctcac
     8701 ggaggacatg gtccgcgagt cgaaccagcc gtccggcccg atcccggtcg ttccggaagg
     8761 ggcgcccgcc ctcgacgagt cgggcctctt ctccgacgag caggagttct tcaacttcgc
     8821 cgacgagctc cataccgagc tccggaagga aggggagacg gcgccggcgg aagagggccg
     8881 ggaggtctcg ctcgaggaga tcttccgcga cttcaagaag ggggtcgagc agcagctctc
     8941 tcccgaggac tacgagaccc actacaacct cgggatcgcc tacaaggaga tggggctcac
     9001 ggacgaggcc atcggcgagt tccagctcgc ggcgaaggac ccgctccacg ccgtggagtg
     9061 ctgcgcgatg ctcgggcttt gcttcctcga gaagggtctc ccgcagctcg cggtgaagtg
     9121 gtaccgcaag ggactcgaca ccgtcggcat caaggacgac gaccggctcg gcctccagta
     9181 cgccctcgcc ggggtcctcg agcagatcgg agacgccgag ggcgcctacc ggacctacct
     9241 cgagatcttc agctccaacg cggcctaccg ggacgttccc gcccggatca aggagctccg
     9301 cccgatcatc gcgccctgac ggcgcccgtc gccaggcctc cttcgggatc gttccgttcg
     9361 cgccggcgga ctcgagcgca accgtccgcc gcgccggtat catcggagct ccgatgacgc
     9421 gcgacggtgc tgccggcccg ggcgacgccc cgctcctcac cgtgcgctcc ctcgcggtcg
     9481 acttcgggag cacccgcgtc gtcgacggtg tctccttcca cgtctcccgc ggcgagatcg
     9541 tcgccctggt cggcgagtcg ggctcgggca agacggtcac cgcgctctcg ctgctcgacc
     9601 tcgtcccgat gcccggacgc cgcacggcgg gcgacgtggt cttcgagggc cgggacgtga
     9661 cgggtctctc gccgtcggag ctccgccggc tccgcggagg agggatcggc ttcgtcttcc
     9721 aggagccggg ggaggccctc gacccggtcc ggacgatcgg ttccgagctc gtggccgtcc
     9781 tccggcgcca tcggggcctc tcgcgcgacg aggcgcgccg cgaggctctc tcgtggctct
     9841 cccgggtcgc cctcccggat ccggaggccc ggatgcggga cctccctcac cggatgtcgg
     9901 gagggatgcg gcagagggcg atgat
//
LOCUS       DAOTEZ010000126         9921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4817_length_9921_cov_6.475471, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000126 DAOTEZ010000000
VERSION     DAOTEZ010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9921
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4817_length_9921_cov_6.475471"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="P5164_08315"
     CDS             <1..726
                     /locus_tag="P5164_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRY43935.1"
                     /translation="WSTIKHKKGAADAKRGRLFTKLIKEITMSAKVGGGVVESNPRLR
                     SAVLAAKKASMPSDNIDKAIKRGTGELEGVTYEEVVYEGYGPGGAAILIEAMTDNKNR
                     TTAEVRHLLSKYGGNLGATGSVAFQFSKKGYIAVEKSSAAEDALMEAALEAGAEDFQA
                     EDSDVYEIYTDLASFETVKAQLEAKKFVLQTAELQMIPATMVAVGDKAPALLKLLDML
                     EDNDDVQKVWSSGDIDDQYLQDA"
     gene            826..1365
                     /gene="ruvC"
                     /locus_tag="P5164_08320"
     CDS             826..1365
                     /gene="ruvC"
                     /locus_tag="P5164_08320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRY43936.1"
                     /translation="MRILGVDPGSRAAGWAIVSFDGAPRLTAAGVLRPATTSSFAERL
                     LALHDALLAVVERERPDEAAIERVFSGVNPQSLITLGEARGVLLLALARAGLPVHEVT
                     PAEIKKTVTGTGQAGKDQVRRMVLALVGAPAGSSRLAFDAADAAAAALTHGFRTSAGR
                     RGLRSVPPSSSSVRPRVAG"
     gene            1415..2308
                     /locus_tag="P5164_08325"
     CDS             1415..2308
                     /locus_tag="P5164_08325"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HRY43937.1"
                     /translation="MSFQGSLKELPLADIVQLVAVSGKTGMFSLTRAAEKGAVYIQNG
                     QITHAKVNDVEGEDAIYALALWNEGQFQFSAGVESETRTITRSNTNLLMEAARRSDEW
                     KILSKKIPTTEAVPQLVCREGLSEPVTLTPNEWLVVTGTDGARTIEEIARATRLSAFD
                     VAKTLYGLITAELVEIRTKAEARPAAPPSGPAASAPGQASGPVVTTHSGVFNAVTRGD
                     ERRSLQILCTKVKQEAESAALAPGDAAIDRLYRAALGEVDKGRGLDVVRELIRSLEQT
                     VAALRGRDASLAFREKMAPLL"
     assembly_gap    2458..2557
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2710..3843
                     /locus_tag="P5164_08330"
     CDS             2710..3843
                     /locus_tag="P5164_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43938.1"
                     /translation="MRRALVLLAFAAAPLLAEEPVREEPAGLIPRIAVFVPEGEAEIR
                     LSKLVRSSLFETQFEYDFVSGDIAAFLRYKYYGSRQSFTLAAFDRVSFAALESLSLGY
                     DRVRGLSAFVRRPVGLYGRLVFLAEADRFNVSGPKKPHESADDGQTNLFGKVGFQWGT
                     EDDDFGNRISGDPNDRVRNLFTVARAFAPGGRGLSLGVTYGAPVLDYDYLKVEGEAIQ
                     AVDVGTSRLVARFHAGFFPHKPPSDGETLPGGLPYRIPAGEYFSLGGRDALRGLRDAP
                     YGTDELHVTVEAFVPVFVNRNAQFLKATWNTLYAVGYVGAGNAGVGRDVYTRLGDYRQ
                     DAGLGFEVAFRVSRYRVFASALAAREIGGGGSPRFLMTLRTVN"
     gene            3970..5322
                     /locus_tag="P5164_08335"
     CDS             3970..5322
                     /locus_tag="P5164_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRY43939.1"
                     /translation="MRRMRSGLLLALAVALLAALPLSAQVAPTLTGETGLFGIQDADT
                     VPQGKFAFSLFYSMYDRTAAPVPLLAPLGDDPLRYSTDKLGLSITFGLLPNWEASFTA
                     GQRYYSSDDRLWAGNIGGRNRVGEVDHDETDKLRIGTKYVLNRKDPLKVAIFGAAYIP
                     TQRKNDDAAMSSYRTDWEWGFSGTYGIFSGQFAYLWTADHDTEYDPYEIPNEMTFSAG
                     VGVPLIPDRLKGIFEIHRVMYDGGTTEPPDFTDAVLGARFALGKSGLVAGAGIRANID
                     RWVKYGSSPSNVGGLLQLSYAPQPEKVVAAPKVVPSPHVPPPVEPAPAPVLEPAPAPV
                     VEAPPAPKPETSTTDEILFDSARSRLTNIAKAILDGVALRLKNNLSATCAIAGHTDPG
                     EKGDRAALAKARADAAKDYLVKRHGIDASRIQVDGRGDAEAGGDATRNRRAVVTVTFP
                     "
     gene            5413..6561
                     /locus_tag="P5164_08340"
     CDS             5413..6561
                     /locus_tag="P5164_08340"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRY43940.1"
                     /translation="MDSSGAFTTGTSPALGGVFRSRALVVLATAAVVGLLWAGKILFV
                     TIFFALFLSFALHPFVELLEKLRVPRGLAAFVVLLLLCALFAFFIVNALDQAQAFSRD
                     WPSYERKIQALTAQARGVFSRIEEGTRRLLSDGGASGVRSVRLEEGVLASISRVALQA
                     KTVFSLLLYTAAVPMLAFFMLKDREKYSGALTRILRRKGGRAASDFAVNVAHVLSGYV
                     LGELFVVLITACITTAGLLILKVPYSYVLGPLAGLCVLLPYVGVILSTTPAVFVAFLG
                     TDEPFLALKVVAFYSVVQFLEGNVLTPFIVGSRVRLHPLAVLVAFLFWGILWGLPGAI
                     LAVPLTATVKVVGERFERFAPWAALLGDRTPDPEPEPEEPKPEPEAAG"
     gene            6566..7234
                     /locus_tag="P5164_08345"
     CDS             6566..7234
                     /locus_tag="P5164_08345"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HRY43941.1"
                     /translation="MCGRYVQTSPADALAARFGARLPEGLAWVPRWNVAPSTPAPVVR
                     AAGTGRELALLTWGLLPSWAKEGVSLRPINARAEGLAGKPMFRDALRRRRALVPADGF
                     YEWRVSGRGKTPWHFRLKDGEPFAFAGLWELREAPGGPALETFAIVTTEANDVVRPVH
                     ARMPVILDAEAEALWMREGALSPEEAARCLRPLDPSRMEDWAVSSWVSSPAHDGPRCV
                     EPAG"
     gene            7304..8551
                     /locus_tag="P5164_08350"
     CDS             7304..8551
                     /locus_tag="P5164_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43942.1"
                     /translation="MHPDFRAAFNRAFSPALARGQAEDLAGRAGRVPGFRLAETPVFL
                     TRELDERLVAASHGILAQLSRPETVARLRRFVPPEWDAPGMDALPSIAQVDLAVTRAA
                     DGSLEPKLVELQGFPSLSAFEVLQADAWEAALAGIPGLPAGGWSPYFSGLTRESYLDL
                     FRRTVVARHDPETVVLLDLRPDRQKTAVDFDATRKLLGVEAVCATEIEREGRRLYRRK
                     GGRRVPIERIYNRVVFDELARSGDRLPFDFRDDLAVEWAPHPNWFWAFSKAALPFLDH
                     PAAPRARTLETLDALPADLETGWVLKPLFSFAGGGVNVEPTESDVMAVPAAERGRWCL
                     QEKIDYAPALAAADGGGVKVEIRMMFFRPDAEPAFTLGLNLCRLSRGKMHGVDFNRDF
                     TWVGSSVALRPAAGWPEPGARGA"
     gene            8606..9166
                     /locus_tag="P5164_08355"
     CDS             8606..9166
                     /locus_tag="P5164_08355"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRY43943.1"
                     /translation="MPQNLKGLKVLFLVAPKGFQAEELEKPRRVLEAAGAEVVVATTA
                     GGGAKAQDGTAIDAVPIVELRPTDFAGAVVVGGPGAPDLWENGIAHKFFRMTTHDGKP
                     VGGISLGVGLLAKAGLLEGKSATIWVTPEALRTLKDGGARYEKKPVVVAGGVVTADGP
                     SSAESFGNIFSELLDGSRSRSGRVTR"
     gene            complement(9185..>9921)
                     /locus_tag="P5164_08360"
     CDS             complement(9185..>9921)
                     /locus_tag="P5164_08360"
                     /inference="COORDINATES: protein motif:HMM:NF020134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HRY43944.1"
                     /translation="QCAGFLSGLQTADAFIRSGAFDRVLLVGAEVHSGLMPWPESVWD
                     VMLGRSPGPCDAYWYERATGMRDRAVLFGDGAGAMVLEANDSGDGRGFLGIVLKTDGN
                     HWDKLYVPGGGCKSRPYFTPAMFESLGTIPIVEGRQVFRLASLLMPEAVREVLAASGH
                     ALEELHLLLMHQANLRINESVQRVLGLPDEKVFNNIQKYGNTTAATLPLVYDEAVEAG
                     RIGPGALVGFTALGAGLHWGAALARI"
BASE COUNT         1364 a         3676 c         3322 g         1459 t
ORIGIN      
        1 tggagcacga tcaagcacaa gaagggcgcc gccgacgcca agcgcggccg gctcttcacg
       61 aagctgatca aggaaatcac gatgtccgcc aaggtcggcg gaggcgtcgt cgagtcgaac
      121 ccgcgccttc gcagcgcggt gctcgccgcg aagaaggcgt cgatgccttc ggacaacatc
      181 gacaaagcga tcaagcgggg aacgggcgag ctcgaagggg tcacctacga ggaggtcgtc
      241 tacgagggtt acgggcccgg cggggcggcg atcctcatcg aggcgatgac cgacaacaag
      301 aaccggacga cggccgaggt tcgccacctc ctctcgaagt acggcgggaa cctgggggcg
      361 acgggatcgg tcgcgttcca gttctcgaaa aagggctaca tcgccgtcga gaagagctcc
      421 gccgccgagg acgcgctcat ggaagcggcg ctcgaggccg gcgccgagga cttccaggcc
      481 gaggattccg acgtctacga gatctacacc gacctcgcgt cgttcgagac ggtgaaggcg
      541 cagctcgagg cgaagaagtt cgtcctccag accgcggagc tccagatgat ccccgcgacg
      601 atggtcgccg tcggcgacaa ggcgcccgcg ctcctgaagc tcctcgacat gctcgaggac
      661 aacgacgacg tccagaaggt ctggtcgagc ggcgacatcg acgaccagta cctccaggac
      721 gcgtaggctc tccggggggg cgctgctcgc cgccccccgg acccccgacg acaaggcgat
      781 ggacggaccg tcgattctct ctcacgctct gagtcccgtg cggcggtgag gatcctcggc
      841 gtcgacccgg ggagccgcgc cgccggctgg gcgatcgtct cgttcgacgg cgcgccgcgc
      901 ctcacggccg cgggcgtcct gcggccggcc acgacctcct ccttcgccga gcggctcctc
      961 gcgctccacg acgcccttct cgcggttgtc gagcgggagc ggccggacga ggccgcgatc
     1021 gagcgcgtct tctcgggcgt caaccctcag agcctcatca cgctcggcga ggcgcgcggc
     1081 gtcctcctcc tcgcgctcgc ccgggccggc ttgccggtcc acgaggtcac gccggcggag
     1141 atcaagaaga ccgtgacggg gaccggccag gccggcaagg accaggtccg gcggatggtc
     1201 ctcgctctcg tcggcgcgcc ggccggctcg tcccggctcg cgttcgacgc ggccgatgcc
     1261 gccgccgcgg ccctgaccca cggcttccgg acctccgccg gccgccgcgg cctccggtcc
     1321 gtgccgccct cctcctcgtc cgtccgcccg cgcgtcgctg gttgagcatc cccggggggg
     1381 gtgctatcgt cccgtctcgc gagaacgcgc acgcatgtct ttccaaggct ccctcaaaga
     1441 actcccgctc gcggacatcg ttcagctggt cgccgtatcg gggaagaccg ggatgttttc
     1501 cctcacgcgc gccgccgaaa agggcgcggt ctacatccag aacggccaga tcactcacgc
     1561 gaaggtcaac gacgtcgagg gcgaggatgc gatctacgcg ctcgcgctct ggaacgaagg
     1621 gcagttccag ttctccgcag gcgtggagag cgagacgcgg acgatcaccc gctccaacac
     1681 gaacctcctg atggaggccg cgcgccggag cgacgagtgg aagatcctct cgaagaagat
     1741 ccccaccacc gaggcggtgc cgcagctcgt ctgccgcgag gggctgagcg agcccgtcac
     1801 gctgaccccg aacgaatggc tcgtcgtcac cgggaccgac ggcgcccgga cgatcgagga
     1861 gatcgcccgc gcgacgcgcc tctcggcgtt cgacgtcgcc aagacgctct acggcctgat
     1921 caccgccgag ctcgtcgaga tccgcacgaa ggccgaggcc cggccggccg cgcccccctc
     1981 cggcccggcg gcgtccgcgc ccgggcaggc ctccggcccg gtcgtcacga cgcacagcgg
     2041 cgtcttcaac gccgtcaccc ggggtgacga gcgccgcagc ctccagatcc tctgcacgaa
     2101 ggtgaagcag gaggcggaga gcgccgccct cgcgccggga gacgccgcga tcgaccgcct
     2161 ctaccgcgcc gccctcggcg aggtggacaa ggggcgcggg ctggacgtcg tccgcgagct
     2221 gatccggagc ctggagcaga ccgtcgccgc cctgcgcggt cgcgacgcga gcctcgcttt
     2281 ccgcgagaag atggccccgc tgctgtaagt gttctcggga aacccgcgcc cgggtttccc
     2341 gaacccttcc gcggcccgga gcaccagagc ttccccttct cctgcccgtc tctgcagaga
     2401 ccggaaaccc gcgcccgggt ttcccgaacc cttccgcggc ccggagcacc agagcttnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnagg gcttcccctt ctcctgcccg
     2581 tctctgcaga gaccggagac ccgcgcccgg gtctgactgc gtcttcgaac gggaccgctt
     2641 ccgcgctccc attcggtcct cgaacccttc cgcggaacgc gcgggcgcgc ctctctatac
     2701 tcgtcgcgct tgcggcgtgc gctcgtcctc ctcgctttcg cagcggctcc gcttctcgcg
     2761 gaggagccgg ttcgcgagga gcccgcgggc ctcatcccca ggatcgccgt cttcgtcccc
     2821 gagggtgaag cggagatccg gctctcgaag ctcgtccgca gctcgctctt cgagacgcag
     2881 ttcgagtacg acttcgtctc gggcgacatc gcggcgttcc tgcgctacaa gtactacggc
     2941 tcccgccaga gcttcacgct cgcggcgttc gaccgggtct ccttcgcggc gctcgagtcg
     3001 ctctccctcg gctacgaccg agtgcgcggc ctctcggcgt tcgtcaggcg gcccgtcggg
     3061 ctctacggcc gcctggtctt tctcgcggag gcggaccgat tcaacgtctc cggcccgaag
     3121 aagccccacg agtccgccga cgacggccag acgaacctct tcgggaaggt cggctttcag
     3181 tggggaacgg aggacgacga cttcgggaac cggatctccg gcgacccgaa cgaccgggtg
     3241 aggaacctct tcacggtggc gcgggcgttc gccccgggcg ggagaggcct ctccctcggg
     3301 gtcacctacg gcgcgccggt cctcgactac gactacctga aggtcgaggg cgaggcgatc
     3361 caggcggtcg acgtcgggac cagccgtctc gtggcccggt tccacgccgg cttcttcccc
     3421 cacaagccgc cgagcgacgg cgagacgctc cccggagggc tcccctaccg gatcccggcc
     3481 ggcgagtact tctcgctcgg gggacgcgac gcgctgaggg ggcttcgcga cgctccgtac
     3541 gggacggacg agctccacgt gacggtcgag gcgttcgtcc ccgtcttcgt caaccgaaac
     3601 gcccagttcc tgaaggccac ctggaacacc ctgtacgcgg tcggctacgt cggagcgggg
     3661 aacgcagggg tcggacgcga cgtctacacc cggctcggcg actaccggca ggacgccggc
     3721 ctcggattcg aggtggcctt ccgggtctcc cggtaccggg tcttcgcctc cgccctggcg
     3781 gcccgggaga tcgggggggg cggttcgccc cgatttctga tgacccttcg gacggtcaat
     3841 tgactttgcc gggaaatccc gtgctgctag actcttgaca actgatccga gcccaggacg
     3901 cggagcggct tccccttccc cggtacgggg gtgattcgtc cggtccccgg ctggaaataa
     3961 ggagactcga tgcgacggat gcggtccgga ctcctcctcg cgctggccgt ggctcttctc
     4021 gcggcgcttc cgctgagcgc tcaggtggct cccaccctca ccggcgagac cgggctcttc
     4081 ggaatccagg acgcggacac ggtcccccag ggcaagttcg cgttctcgct cttctacagc
     4141 atgtacgacc gaaccgccgc gccggtgccg ctcctggcgc cgctcgggga cgacccgctc
     4201 cggtacagca ccgacaagct cgggctgtcg atcacgttcg gcctcctgcc gaactgggag
     4261 gcgtcgttca cggccgggca gcggtactac tcctcggacg accggctctg ggccgggaac
     4321 atcggggggc ggaaccgcgt cggcgaggtc gaccacgacg agaccgacaa gctccggatc
     4381 gggacgaagt acgtcctcaa ccggaaggac ccgctgaagg tcgccatctt cggcgccgcc
     4441 tacattccga cccagcggaa gaacgacgac gccgcgatgt cctcctaccg gaccgactgg
     4501 gagtggggat tctcggggac ctacgggatc ttctcggggc agttcgccta cctctggacg
     4561 gccgaccacg acacggagta cgacccgtac gagatcccga acgagatgac gttctccgcc
     4621 ggcgtcggcg tcccgctgat ccccgaccgg ctgaagggga tcttcgagat ccaccgcgtc
     4681 atgtacgacg gcgggacgac cgagcctccc gacttcaccg acgccgtcct cggcgcgcgc
     4741 ttcgcgctcg gcaagagcgg cctcgtggcc ggcgccggga tccgcgcgaa catcgaccgg
     4801 tgggtgaagt acggcagctc gccgagcaac gtcggcggcc tcctccagct ctcctacgca
     4861 ccgcagcccg agaaggtcgt cgcggctccg aaggtcgttc cctcgcctca cgtcccgccg
     4921 ccggtcgagc ccgccccggc ccccgtcctc gagccggccc ccgcgcccgt cgtggaggcc
     4981 ccgcccgcgc cgaagcccga gacctcgacg accgacgaga tcctcttcga ctccgcccgg
     5041 agccgcctga cgaacatcgc gaaggcgatc ctcgacggcg tggcgctccg gctgaagaac
     5101 aacctctcgg cgacctgcgc gatcgccggc cacaccgacc cgggcgagaa gggcgaccgc
     5161 gccgcgctcg cgaaggcccg cgccgacgcc gcgaaggact acctcgtcaa gcgccacggc
     5221 atcgacgcct cccggatcca ggtcgacggc cggggcgacg cggaggcggg cggcgacgcg
     5281 acgcgcaacc ggcgcgccgt cgtcacggtg acgttcccct agcccgaagc cccggcttcc
     5341 cctgcgacgt tcgagcggcc ggcctttcgc cggccgctct cgtctttccc cgacgcgcta
     5401 aactggcccc gcttggacag cagcggcgcc ttcacgaccg gaacgagccc cgctctcggc
     5461 ggggtttttc gttcgcgcgc gctcgtcgtg ctcgcgacgg cggccgtcgt cggcctgctc
     5521 tgggccggga agatcctctt cgtcacgatc ttcttcgccc tcttcctctc gttcgcgctc
     5581 cacccgttcg tggagctcct cgagaagctg cgcgtcccgc gcggcctcgc ggcgttcgtc
     5641 gtcctcctcc tcctctgcgc gctcttcgcg ttcttcatcg tcaacgcgct cgaccaggcc
     5701 caggccttct cgcgcgactg gccgtcctac gagcggaaga tccaggcgct cacggcgcag
     5761 gcgcgcggcg tcttctcgcg catcgaggag ggaacgcgcc ggctcctctc cgacggtggc
     5821 gcctccgggg tccgttcggt ccgcctggag gaaggggtgc tcgcctcgat ctcgcgcgtc
     5881 gcgctccagg cgaagacggt cttctcgctc ctcctctaca ccgcggcggt cccgatgctc
     5941 gcgttcttca tgctgaagga ccgcgagaag tactccggag cgctcacgcg catcctccgc
     6001 cggaagggcg gccgcgccgc ctccgacttc gccgtcaacg tcgcccacgt cctctcgggc
     6061 tacgtcctgg gcgagctctt cgtcgtcctg atcaccgcgt gcatcacgac cgccggcctc
     6121 ctcatcctga aggtcccgta cagctacgtg ctcgggccgc tcgccgggct ctgcgtcctc
     6181 ctcccgtacg tcggcgtcat cctctcgacg acccctgcgg tcttcgtggc attcctcggc
     6241 accgacgagc cgttcctcgc cctgaaggtc gtcgcgttct actcggtcgt gcagttcctc
     6301 gaagggaacg tcctgacgcc gttcatcgtg gggagccgcg tccgcctcca cccgctcgcg
     6361 gtgctcgtcg cgttcctctt ctgggggatc ctctggggcc tccccggggc gatcctcgcc
     6421 gtgccgctca cggcgacggt gaaggtcgtc ggcgagcggt tcgagcgctt cgcgccgtgg
     6481 gcggcgctcc tcggcgaccg gacgcccgac cccgagccgg agcccgagga gccgaagccg
     6541 gagccggaag cggcggggtg agcccgtgtg cggccgctac gtccagacga gcccggccga
     6601 cgccctcgcg gcgcgcttcg gcgcccgcct gccggagggg ctcgcgtggg tcccgcgctg
     6661 gaacgtcgca ccgtcgacgc cggcgcccgt cgtccgcgcc gccggaaccg ggcgtgagct
     6721 ggccctcctg acctggggcc ttctcccctc gtgggcgaag gagggcgtct ccctccgccc
     6781 gatcaacgcg cgcgccgagg ggctcgccgg aaagccgatg ttccgcgatg cgctccggcg
     6841 ccgtcgcgcg ctcgtccccg ccgacggatt ctacgagtgg cgggtctccg ggcggggcaa
     6901 gactccctgg catttccggc tgaaggacgg agagccgttc gcgttcgccg gcctctggga
     6961 gctccgcgag gcgccgggcg gcccggccct cgagacgttc gcgatcgtga cgacggaggc
     7021 gaacgacgtc gtccggcccg tccacgcgcg gatgccggtg atcctcgacg ccgaggccga
     7081 ggcactctgg atgcgcgagg gcgcgctttc ccctgaggaa gcggcgcgct gcctccgccc
     7141 gctcgatccc tcgaggatgg aggactgggc cgtctcctcc tgggtgagct ccccggcgca
     7201 cgacgggccg cgctgcgtcg agccggcggg ctgaagccgc gtttcgcgga cggcgcagcg
     7261 cgtccttccc ttcctccggg cgcctcccta gactgccctc ccgatgcacc ccgacttccg
     7321 cgccgccttc aaccgcgcgt tctcccccgc gctcgcccgc ggccaggccg aggacctcgc
     7381 ggggcgcgcg gggcgcgtgc ccggattccg cctcgccgag accccggtct tcctgacgcg
     7441 cgagctcgac gagcggctgg tcgcggcgtc gcacgggatc ctggcgcagc tctcccgccc
     7501 cgagaccgtc gcgcggctgc ggcggttcgt cccgcccgag tgggacgccc ccggaatgga
     7561 cgcgctcccg tcgatcgcgc aggtcgacct cgccgtgacc cgggccgccg acgggagcct
     7621 cgagccgaag ctcgtcgagc tccagggctt cccgtccctc tccgccttcg aggtgctcca
     7681 ggccgacgcc tgggaggccg ccctcgcggg gatcccgggg ctccccgccg ggggatggag
     7741 cccctacttc tccggcctga cgcgggagtc gtacctcgac ctcttccgca ggaccgtcgt
     7801 cgcgcgccac gaccccgaga ccgtcgtcct cctcgacctc cgccccgatc gccagaagac
     7861 ggccgtcgac ttcgacgcga cgcggaagct gctcggcgtc gaggccgtct gcgcgaccga
     7921 gatcgaaagg gaggggcgcc gcctctaccg acggaagggc ggtcggcgcg tcccgatcga
     7981 gcggatctac aaccgcgtcg tcttcgacga gctggcccgc tccggggacc ggctcccgtt
     8041 cgacttccgc gacgacctcg cggtcgagtg ggcgccccac ccgaactggt tctgggcctt
     8101 ctcgaaagcc gccctgccgt tcctcgacca cccggccgcg ccgcgggcgc ggacgctcga
     8161 gacgctcgac gccctcccgg ccgacctcga gacggggtgg gtcctcaagc ccctcttctc
     8221 cttcgcgggg gggggcgtca acgtggagcc gacggaatcg gacgtcatgg cggtccccgc
     8281 cgccgagcgg ggccgctggt gcctgcagga gaagatcgac tacgcgcccg cgctcgcggc
     8341 cgcggacggc ggaggggtga aggtcgagat ccggatgatg ttcttccggc ccgacgcgga
     8401 gcccgcattc accctcggcc tgaacctctg ccggctctcc cgcgggaaga tgcacggcgt
     8461 cgacttcaac cgcgacttca cctgggtcgg ctcctcggtg gcgctccgcc cggctgcggg
     8521 gtggccggag ccgggcgcgc gcggcgcttg acaggagcgg tccgcccgga gatcgtcgcg
     8581 caaacagacg tcgcgggagg ctcacgtgcc gcagaacctg aagggtctga aagtgctgtt
     8641 cctcgtcgct ccgaagggct tccaggccga ggagctcgaa aagccgcgcc gcgtcctcga
     8701 ggcggccggc gcggaggtcg tcgtcgccac gacggcggga ggcggggcga aggcgcagga
     8761 cggcaccgcg atcgacgccg ttccgatcgt cgagctgcgg ccgaccgatt tcgccggtgc
     8821 ggtcgtcgtc ggcggtcccg gcgcgccgga cctctgggag aacgggatcg cgcacaagtt
     8881 cttccggatg acgacgcacg acggcaagcc ggtcggaggg atctccctcg gcgtcggcct
     8941 tctcgcgaag gccggcctcc tggaagggaa gtcggcgacg atctgggtca ccccggaggc
     9001 cctgcgcacg ctcaaggacg gaggggcgcg ctacgagaag aagcccgtcg tcgtcgcggg
     9061 cggcgtcgtg accgccgacg gcccctcctc cgccgagagc ttcgggaaca tcttctccga
     9121 gctcctcgac ggctcgcgca gccgctccgg gcgcgtcacg cgctgacggc ggcgcgcctc
     9181 cccgtcagat ccgcgcgagc gcggcgcccc agtggaggcc cgcgccgagg gcggtgaacc
     9241 cgacgagcgc ccccggaccg atccgtcccg cctcgaccgc ctcgtcgtag acgagcggga
     9301 gggtcgccgc cgtcgtgttg ccgtacttct gaatgttgtt gaagaccttc tcgtccggca
     9361 ggccgaggac cctctggacc gactcgttga tccggaggtt cgcctggtgc atcaggagca
     9421 ggtggagctc ctccagagcg tgaccgctcg cggcgaggac ctcccgcacc gcctccggca
     9481 tcaggaggct cgcgaggcgg aagacctggc gcccctcgac gatcgggatc gtgccgaggc
     9541 tctcgaacat cgccggcgtg aagtagggac gcgacttgca gcccccgccc gggacgtaca
     9601 gcttgtccca gtggttcccg tcggtcttca ggacgatccc gaggaagccc cggccgtccc
     9661 cggagtcgtt cgcctcgagg accatcgcgc cggcgccgtc gccgaagagg acggcccggt
     9721 cccgcatccc cgtcgcccgc tcgtaccagt acgcgtcgca cggccccggg ctcctcccca
     9781 gcatgacgtc ccagaccgat tcgggccagg gcatcaggcc cgagtggacc tccgccccga
     9841 cgaggaggac gcggtcgaat gcgccggagc ggatgaaggc gtcggccgtc tggagcccgg
     9901 agaggaagcc ggcgcactgc a
//
LOCUS       DAOTEZ010000127         9918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4821_length_9918_cov_5.550745, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000127 DAOTEZ010000000
VERSION     DAOTEZ010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9918
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4821_length_9918_cov_5.550745"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..270
                     /locus_tag="P5164_08365"
     CDS             1..270
                     /locus_tag="P5164_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012070121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRY43945.1"
                     /translation="MIEKTVVLKNRLGLHARAAAKLVHTAAHYASRITLSKDGEDVDG
                     KSILGLLLLAAGKGTPIVVRTEGADEAEAMRALSDLVDRRFDESE"
     gene            300..2087
                     /gene="ptsP"
                     /locus_tag="P5164_08370"
     CDS             300..2087
                     /gene="ptsP"
                     /locus_tag="P5164_08370"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HRY43946.1"
                     /translation="MKRSLSLTGIPVSPGIAVGRAVVCIGRTDSTPRRDLDVEEVPAE
                     LERLRLAVESAIREIGEMAGQVEARLGKEYAAIFQAHALFLRDPSFLGPIEKKVRVEK
                     VNAEWAVEVVADALAARLRDLPDKDLALRASDLDDIATILKRGLGEGSGAIDRLATLP
                     WDSCVLVADELTPSDAVRIPRDKVVAFVTERGGKTSHAAILARSFGLPAVVAVPKLLA
                     SVGEGDQLIVDGREGVLWRAPSEDVLSLFRERQGRDATRELSLKEKSLSGLARTRDGE
                     EVAIRANIELAREVPDVFEYGADGVGLFRSEFLYLSSAGVEFPDEVTQAAIYRQVVSQ
                     LSPRPVVIRTYDLGGKKGARHLSGAEENPVLGLRGVRLCFSHPEMFRTQLRALLSVAS
                     EGDLRILVPMVAGVEEVRRVRSFVEEARSELADRGVVVPDSVPIGAMIEVPSAAITAD
                     FIAPEVDFLSLGTNDLIQYTLAADRANEAVSDIFRPQHPAILRLIARVQEAARAAGKP
                     LAVCGEMAADPALFLLLLGLGIREFSMGPRSVPLLKDVARCASAADAARMARAALSLS
                     TPDDVAALLAREAEALLSGDPETRAAVNA"
     gene            2084..2452
                     /locus_tag="P5164_08375"
     CDS             2084..2452
                     /locus_tag="P5164_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRY43947.1"
                     /translation="MKTTDRIVKKPWGSERIFAVNSRYAGKLISILAGQTLSYQYHRA
                     KEETIHVLSGTLGMETESNGERILLALAPGETFHVMPGTRHRMFAEGEDVLVVEVSSP
                     ELDDVVRLEDRYGREGTTQP"
     gene            2821..3144
                     /locus_tag="P5164_08380"
     CDS             2821..3144
                     /locus_tag="P5164_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43948.1"
                     /translation="MPSEIPEYRDASGRTEAEWRQRAEEIRSRAAEAEAEVAAARAEV
                     RRLENDFYAWSDGNYRERVIRPAWDQARERLKGLEAAAEDARKAMDGLEEEARKSGTP
                     PGWLR"
     gene            3132..4544
                     /gene="miaB"
                     /locus_tag="P5164_08385"
     CDS             3132..4544
                     /gene="miaB"
                     /locus_tag="P5164_08385"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRY43949.1"
                     /translation="MASLNDGRLAAPGEADAGGRTRKVFVETWGCQMNELDSRRFVGL
                     MSRAGWEESAEPTDADLVLLNTCSVRDKAEQKVYDYLGRVALLKKTRPGMLLGVCGCV
                     AQQEGEEILRRSPAVDFVLGTGRIELLPAVVRRVEREGDRPVETGFDLDEVAYTPGAV
                     ARTVAHRASITIIEGCNKHCTFCVVPTTRGRERSRRSSEVVEECRRLVGEGVVEIELL
                     GQTVNAYSDPESGAGLSALLRAISAIPGLRRLRFVTSHPRNFGDDLIAAMAECEPVCP
                     SLHLPFQSGSDSVLRRMKRQYSRAEYVDLAGRLRSAVPGLALSTDIIVGFPGETDEDF
                     ESTITLLDEVRFGAIFCFVYSPRPATAAARWELDVPAPVAQARLAKLNDHQQDIQLRA
                     NEAMVGEVLEVLVEGPDRTGKKVSGRSPYNHLVHVENAQGTPAGSFIHARIEQGLANS
                     LLGRALRVERGTPSGPNPST"
     gene            4591..5076
                     /locus_tag="P5164_08390"
     CDS             4591..5076
                     /locus_tag="P5164_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HRY43950.1"
                     /translation="MSVKMDVKALIVDPIANMPVVILRDAEDRSFLPIWVGVFEANAI
                     ALQLEGVRTPRPMTHDLLRQTITQLDGTVEHVVITRLHENTFYAEIHLTSGGRTVVVD
                     SRPSDAIALALRSSAPVFVEESVLEKSQAQDDSSEAQSHERLRKWFEEADPESLGKYK
                     M"
     gene            5191..5946
                     /locus_tag="P5164_08395"
     CDS             5191..5946
                     /locus_tag="P5164_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRY43951.1"
                     /translation="MIIAITNQKGGVGKTTTAINVAAALAQKNLRTLLVDLDPQGNST
                     MSYLDRRSIGGSMYEVLVNPEVTVSSIVRPTALPGLSIAPAKISLAKADSKLIGEIDG
                     HYRLKDKMTDVASDYDAVIIDTPPTLGMITVNAMVASTHIIIPIQSSYFALEGTDDLL
                     ETIEKIKSRPNPNLQILGVVITLHDRRTVLGRDIQKQIDTVFQGKIFKTTISKSVRLE
                     ESPAYRESIFTFAPRSTGASEYYSLTEEILARV"
     gene            5975..6895
                     /locus_tag="P5164_08400"
     CDS             5975..6895
                     /locus_tag="P5164_08400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRY43952.1"
                     /translation="MRHDPHFVDQLFKGEEISIGRKIALNLIEANPDQPRSMLGDLGD
                     LKASIEARGVLEPILVRPRDDGRFTIISGERRFRAALEAGLSEVPCIVFDVSDAEVLE
                     IALIENLQRKDLSPLEEADGYLTLQEKHGYTHEQIARAVGKSRVTITETLSLARLPLR
                     VKDECRRADIRSKSFLLEVGRLPTEPAMLDAIAAFEAGDGVGRDSVRDVRRTASTESP
                     RFRSLRKAYELNFQPADAPYAVSLVFRGNAVERHEIVETLRDLIRKLGSGELDFERDG
                     RFLKSGEKKTAAAGEPSPEDPEELRGVSEG"
     gene            7202..9535
                     /locus_tag="P5164_08405"
     CDS             7202..9535
                     /locus_tag="P5164_08405"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRY43953.1"
                     /translation="MKLTDRYVLKEILAPTLVAFAVYTGFMLVRILVQFSALLLQSAN
                     LPLDVGRVLAFSAPHIVVLTIPIAFLLGILVGVGRLSQDSELVALRASGIDLLGLFRP
                     IAVLASLLALLTGFVMIYVVPRSNSVVFGMRLQLSTFLIAQKIQPGVFSPEVAGRRIY
                     VEKASPDRRTLEGLIISDNSNPSDGERLTIARRGALELEQEEGRLWLRLEDAVTHHTA
                     PDALAYDRASYRTQRLLLDDTNPKERFENTRPDKQLREMTVPELLRRARTTPDPILAR
                     LSWIEVHKKFSFPAACLVFGLVALPLGVVNRRGGRAAGFAMSTAIVVGYYLLYASGES
                     WAIRGSTTPFLAMWLPNILLVALGLWGVSRVRRDRPFLDERAGAALTAAVARALPRAW
                     RPGSRDGKAPENAARPYSGAVGASSWLIDRYVAGRFLRVLALVLTSILLLYLLFEYLE
                     ISDEIARVNPPLSAITGYLRAKLAPILVDVVPIAFAAAALIAIAGLVRSAETTALLSS
                     GVSLFRTTASVLVLAALAGAGAYLFAEQVVPSSAAEADRFKKILVGKPPAAATDAWRS
                     FLKGDSGRFFSAETWDPGSSSLTGVTLFRIDPSTYRLLGKSHAPEARIVPGKGILLSG
                     GWTRTFAEDGSSLFLRHSGPSFVDAPEAARAFLAGRADPRQMNSAELARFIRIRRAAG
                     ADVSAIETGLYQKSSIALAPLLLTLVGLPFAFRYGRRGAVAGIGIALLLGLVYLLLGA
                     ALTTGGETGSLPPLLAAWGTNAFFSLGAGWGLLGVRT"
     gene            complement(9551..>9918)
                     /locus_tag="P5164_08410"
     CDS             complement(9551..>9918)
                     /locus_tag="P5164_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HRY43954.1"
                     /translation="VDRSASYMARHIAKNVVAAGLADRAEVQLAYAIGVADPVSVFVE
                     TFGTERVDPAILPGLIRDHFRLTPKGIIEMLDLKRPIYRKTASYGHFGRRLAEFTWER
                     TDLAEPLAKAAGIAVPASA"
BASE COUNT         1641 a         3505 c         3154 g         1618 t
ORIGIN      
        1 gtgatcgaga agaccgtcgt cctcaagaac cggctcggtc tccacgcccg agcggccgcg
       61 aagctcgtcc acaccgccgc gcactacgcc tcgaggatca cgctgtcgaa agacggcgag
      121 gacgtcgacg gcaagagcat cctcgggctc ctcctcctcg ccgccggcaa ggggactccg
      181 atcgtggtcc ggacggaagg cgccgacgaa gccgaggcga tgagggccct ctcggacctc
      241 gtcgaccggc gcttcgacga gtccgagtag cctcggccgc gaggctacaa tcgaggggca
      301 tgaagcggtc cctctcgctg accggaatcc cggtgtctcc gggcatcgcc gtcggccgcg
      361 cggtcgtctg catcggccga accgactcga cgccccgtcg ggatctcgac gtcgaggagg
      421 tgccggccga gctcgagcgg ctccggctcg ccgtcgaatc ggcgatccgc gagatcggcg
      481 agatggcggg gcaggtcgag gcgcgtctcg gaaaggaata cgccgcgatc tttcaggccc
      541 acgcgctctt cctccgggac ccgtccttcc tcgggccgat cgagaagaag gtgcgggtcg
      601 agaaggtcaa cgcggagtgg gccgtcgagg tcgtcgccga cgccctggcc gctcggcttc
      661 gggaccttcc cgacaaggac ctcgcccttc gggcctcgga cctcgacgat atcgccacga
      721 tcctgaagcg cgggctgggt gaggggagcg gcgccatcga ccgcctggcg accctcccct
      781 gggattcctg tgtcctggtc gccgacgagc tgactccgtc cgacgccgtc cggattccgc
      841 gcgacaaagt cgtcgccttc gtgaccgaaa ggggtgggaa gacctcgcac gccgcgatcc
      901 tcgcccgttc cttcggcctg ccggccgtcg tcgcggtgcc caagctcctc gcgtcggtcg
      961 gagagggaga ccagctcatc gtcgacggac gggaaggggt cctctggcgg gcgccttcgg
     1021 aggacgtcct ctccctcttc cgcgaacgcc agggacgcga tgccacgcgg gagctctcgc
     1081 tgaaggagaa gagcctttcg gggctcgccc gcacgaggga cggcgaagag gtggcgatcc
     1141 gagccaacat cgagctggcc cgcgaggtcc ccgacgtctt cgagtacggc gccgacggcg
     1201 tcggcctctt ccggtccgag ttcctctacc tctcctcggc gggcgtcgag ttcccggacg
     1261 aggtgacgca ggccgccatc tacaggcagg tcgtctccca gctctcgccc cggccggtcg
     1321 tcatccggac gtacgacctc ggtgggaaga agggcgcccg gcacctgtcc ggcgccgagg
     1381 agaaccccgt tctcggcctt cgcggggtgc ggctctgctt cagccacccg gagatgttcc
     1441 gcacccagct gcgcgcgctc ctgtccgtcg cttcggaggg cgacctccgt atcctcgtgc
     1501 cgatggtcgc gggcgtcgaa gaggtccgga gggtccggtc cttcgtcgag gaggcccgca
     1561 gcgagctcgc cgatcgcggc gtcgtcgttc ccgacagcgt cccgatcggg gcgatgatcg
     1621 aggtcccgtc ggccgccatc acggccgact tcatcgcgcc cgaggtcgac ttcctgtcgc
     1681 tcgggacgaa cgacctgatt cagtacacgc tcgcggccga ccgcgccaac gaggccgtct
     1741 ccgacatctt tcggccgcag cacccggcga tcctccgtct catcgcgcgg gtccaggagg
     1801 ccgcccgcgc cgccgggaag ccgctcgccg tctgcggcga gatggccgcc gacccggccc
     1861 tgtttctcct tctcctcggc ctcggcatcc gggagttctc gatgggaccc cggtcggtcc
     1921 cgctcctgaa ggacgtcgcg agatgcgcgt ccgcggccga cgcggcgcgc atggcacggg
     1981 ccgcgctttc cctttcgaca cccgacgacg tcgccgccct tctggcgcgg gaggccgaag
     2041 ccctcctgag cggcgacccg gagacccgcg ccgcggtgaa cgcatgaaga cgaccgatcg
     2101 aatcgtcaag aagccctggg gaagcgagcg gatcttcgcc gtcaacagcc gctatgccgg
     2161 caagctgatc tcgatcctcg ccggtcagac tctctcttac cagtaccacc gggccaagga
     2221 ggagacgatc cacgtcctct cgggaactct cgggatggag acggagtcca acggagagag
     2281 gatccttctg gcgctcgccc cgggggagac ctttcacgtg atgcccggta cgaggcacag
     2341 gatgttcgcc gaaggggagg acgtcctcgt cgtcgaggtc tccagcccgg agctcgacga
     2401 tgtcgtacgc ctcgaagacc gatacgggcg agaagggacg actcagccat gaaacgctcc
     2461 ttccccgcgt cgttcgcgtt cctcgctctt ttcgccggga cggcgcccgg cctcgcgaac
     2521 gcggccgacc cgcagccgac cccgaccccg accgcgacgg ccaagggaaa gaagaagctt
     2581 gccggcggga gcttcggcgt tccgccgaaa ccgaccccga cgccgggatc cgggtccgga
     2641 tcgctggccg atatcgcaag aaaggcgcaa gaagaggaag ccgccgccgc aagggcggga
     2701 cgccggatcg tcatcacgaa cgagagcctc cgaaagtcgg agccggcgca ggggagctcc
     2761 acgatcacga tcacgggctc ggccgacaag aagccggttg cgcggacacg ccccacggcg
     2821 gtgccgtccg agattccgga ataccgtgac gccagcggcc gcaccgaggc ggagtggaga
     2881 cagcgtgccg aggaaatccg ttcgcgagca gccgaagccg aggcggaggt cgccgccgcg
     2941 agagccgaag tccgcaggct cgaaaacgac ttctacgcct ggagcgacgg caactaccgt
     3001 gagcgcgtca tccgtccggc ttgggaccag gcgcgcgaga gactgaaggg cctcgaggcc
     3061 gcagccgagg atgcccggaa ggcgatggac gggctcgagg aagaggcccg taagagcggg
     3121 acccctcccg gatggcttcg ctgaacgacg gccgcctcgc ggcccctggc gaagccgacg
     3181 ccggcgggcg cacgcggaag gttttcgtag aaacctgggg ctgccagatg aacgagctgg
     3241 acagccgcag gttcgtcggg ctgatgtccc gcgcgggatg ggaggagtcg gcagagccga
     3301 ccgacgccga cctggtcctg ctcaacacct gctccgttcg agacaaggcc gagcagaaag
     3361 tctacgacta tctcggccgc gtcgcgctcc tcaagaagac cagacccgga atgctgctgg
     3421 gggtctgcgg ctgcgtcgcc cagcaggaag gggaggagat cctccgccgg tccccggccg
     3481 tcgacttcgt cctcggcacc gggagaatcg agctccttcc cgccgtcgtc cggagggtcg
     3541 agcgcgaggg cgaccgtccg gtcgaaaccg gcttcgacct cgacgaggtg gcctacacgc
     3601 ccggtgccgt cgcccggacc gtcgcgcatc gagcgtcgat cacgatcatc gaagggtgca
     3661 acaagcactg cacgttctgc gtcgttccga cgacgcgcgg ccgggagaga agccgtcgct
     3721 cttcggaggt cgtcgaagaa tgccgtcgcc tcgtcggaga gggcgtcgtc gagatcgagc
     3781 tcctcgggca gacggtcaat gcctactccg acccggagag cggcgccggt ctttcggcgc
     3841 tgcttcgggc gatctccgcg atcccggggc tccgaaggct caggttcgtt acgtcccatc
     3901 ctcgcaactt cggcgacgac ctgattgccg ccatggccga atgcgagccg gtctgcccgt
     3961 cgctccacct tccgttccag tcggggagcg actcggtcct tcgccggatg aagcgtcagt
     4021 acagccgcgc cgaatatgtc gatctcgccg gacggcttcg ttcggccgtg ccggggctcg
     4081 cgctctcgac cgacatcatc gtcgggtttc cgggcgagac cgacgaggac ttcgaatcga
     4141 cgatcaccct cctcgacgag gtccggttcg gcgccatctt ctgtttcgtc tattcgcccc
     4201 gccccgcgac cgcggccgcc cggtgggagc tcgacgtccc cgcccccgtc gcccaggcac
     4261 gcctcgcgaa gctgaacgac caccagcagg acattcagct ccgagcgaac gaggcgatgg
     4321 tcggcgaggt cctcgaagtc ctcgtggaag gtccggatcg gacggggaag aaggtctccg
     4381 ggcgctctcc ctacaatcat ctcgttcacg tcgagaatgc ccaggggacg ccggccggct
     4441 ccttcattca cgccaggatc gagcagggcc tcgcgaactc ccttctcggt cgcgcgcttc
     4501 gcgtcgagcg ggggacgccg tccggtccga acccttcgac ttgaatcgac ggcaccgcga
     4561 acgcagattc gacaggggaa ggagcgcgac gtgagcgtca agatggacgt caaggcactg
     4621 atcgtcgatc cgatcgcaaa catgccggtc gtcatcctcc gcgacgccga ggaccggagc
     4681 ttccttccga tctgggtcgg cgtcttcgag gccaacgcga tcgcccttca gctcgagggg
     4741 gtccggacgc cgcgtccgat gacgcacgac ctcctgcggc agacgatcac ccagctcgac
     4801 ggcacggtcg agcacgtcgt cattaccagg ctccacgaga acacgttcta cgctgagatc
     4861 cacctgactt cgggagggcg aaccgtcgtc gtcgacagca ggccctccga tgcgatcgcg
     4921 ctcgcgcttc gaagctccgc acccgtcttc gtggaggaat cggtcctcga gaaaagccag
     4981 gcgcaggacg actcgtctga agctcagagc cacgagcggc tccggaagtg gttcgaggaa
     5041 gccgatccgg agtctctcgg taagtacaaa atgtagcaga gcgggggagc cgacgacgtc
     5101 ggactcctgc cgtcggcagc tgactcgatt caaacgactt ataaccagcc ttgagaacgg
     5161 gcgccgcagc ccgtatcctc gccttccccc atgatcatcg cgatcacgaa ccagaagggt
     5221 ggagtcggaa agacgacgac cgcgatcaac gtcgccgccg cgctcgccca gaagaacctc
     5281 cgcacgcttc tcgtcgatct cgaccctcag ggcaactcca cgatgtcgta tctcgatcgc
     5341 cgttccatcg gcgggtcgat gtacgaggtt ctcgtgaacc ccgaggtcac cgtctcttcc
     5401 atcgtgcgac cgacggcgct tcccgggctc tccatcgcgc cggcgaagat ctcgctcgcc
     5461 aaagccgact cgaagctgat cggcgagatc gacggacact accgcctgaa ggataagatg
     5521 acggacgtcg cttcggacta cgacgccgtc atcatcgaca cacctccgac gctcggcatg
     5581 atcacggtca acgcgatggt cgcctcgacc cacatcatca ttccgatcca gtcgtcgtac
     5641 ttcgccctgg aggggacgga cgacctcctc gagacgatcg agaagatcaa gagtcgtccg
     5701 aatccgaacc tccagatcct cggcgtcgtc atcacacttc acgaccgccg gaccgtcctg
     5761 gggcgcgaca ttcagaagca gatcgacacc gtctttcagg gaaagatctt caagacgacg
     5821 atctcgaagt ccgtgcgtct tgaggagtcg ccggcgtatc gcgagtcgat cttcacgttt
     5881 gcaccccgct cgacaggcgc ctccgagtac tacagcctga cggaggagat cctcgcccgt
     5941 gtctaagcgt cgtggccttc ccgagacgtc tcgtatgcgg cacgatccgc acttcgtcga
     6001 ccagctcttc aagggcgagg agatctccat tggccggaaa atcgccctca acctgatcga
     6061 ggcgaacccg gaccagccgc gcagcatgct tggagacctc ggggacctga aggcctcgat
     6121 cgaggccagg ggagtcctcg agccgattct cgttcgtccc cgggacgacg ggcgcttcac
     6181 gatcatctcg ggagagcggc gcttccgcgc ggccctcgag gccggtcttt ccgaagtccc
     6241 ctgcatcgtg ttcgacgtga gcgacgccga ggtcctcgag attgctctca tcgagaacct
     6301 ccagcgcaag gacctcagcc cgctcgaaga ggccgacggc tatctcacgc tccaggagaa
     6361 gcacggatac acccacgagc agatcgcccg agcggtaggg aagtcgcgag tcacgatcac
     6421 agagacgctc tctctggctc ggctgcccct ccgggtaaaa gacgaatgtc ggcgtgccga
     6481 cattcgatcg aaatcgttcc tccttgaagt tggacgactg ccaacggagc cggcgatgct
     6541 cgatgcgatc gccgccttcg aagcgggaga cggagtggga agagactccg tgagggacgt
     6601 tcgccggact gcttcgaccg agagcccccg attccggtcg ctccgaaagg cttacgagct
     6661 caatttccag cctgccgacg ctccgtacgc cgttagcctc gtcttccgtg gaaacgcggt
     6721 tgaacggcac gaaatcgtcg agacccttcg agacctcatc cgaaagctgg ggtccggcga
     6781 gctcgacttc gagcgcgacg gccgcttcct caagtccggg gagaagaaga cagcagcagc
     6841 aggcgagccc tccccagagg atcccgagga gctccggggc gtaagcgaag gctaggcccg
     6901 gtctcccggc gccttctgcc tcgcgcgggc gccgaaacgg ccgcgaagtc gctcagcgac
     6961 gacacgttcc gaaagcgaaa gagcctggcg cttggcgctt gacgacgtgt ccatccgtga
     7021 gggagagaaa gccgagcctc gtgctcttcg acccaagcca gccccgaccg aatgttttca
     7081 ggtcgccctg ccattacttt taccataata tttcttatcg gacatatgca ttcgtcgctc
     7141 gggctagaat tcaggcctcc cgagcctttc ctgcgccgcc tgcgtgcgcc tacacctctt
     7201 gatgaagctg accgaccgct acgtcctcaa agagatcctc gcgccgacac tcgtggcgtt
     7261 cgccgtctac accggcttca tgctcgttcg aatcctcgtt cagttcagcg ccctcctgct
     7321 tcagagcgcg aacctgcccc tcgacgtcgg ccgggtgctc gccttctcgg cgccgcacat
     7381 cgtcgtcctt acgattccga tcgcgtttct ccttgggatc ctcgtgggcg tcggacgcct
     7441 cagccaggac tcggagctcg tcgcgctccg ggcctccgga atcgacctcc tcggcctttt
     7501 ccgtccgatc gccgtcctgg ccagcctgct cgcgcttctc accggcttcg tcatgatcta
     7561 cgtggttccc cggtcgaaca gcgtcgtctt cgggatgagg ctgcagctct ccaccttcct
     7621 catcgcccag aagatccagc ccggggtctt cagccccgag gtagcgggac gccggatcta
     7681 tgtcgagaag gcctcaccgg accgcaggac gctcgagggc ctcatcatct ccgacaactc
     7741 caacccgtcc gacggagagc gtctgacgat cgcccggcgg ggcgccctcg agctggaaca
     7801 ggaggagggg cgcctctggc tccggctcga agacgcggtc acccaccaca cggcccccga
     7861 tgccctcgcc tacgaccggg ccagctaccg gacgcagagg ctcctcctgg acgacacgaa
     7921 tccgaaggag cgcttcgaga acacccgccc cgacaagcag ctccgtgaga tgacggtgcc
     7981 ggagctcctc cggcgggcgc ggacgacccc cgacccgatc ctcgcacggc tctcgtggat
     8041 cgaggtccac aagaagttct ccttccccgc cgcgtgcctc gtcttcgggc tcgtcgcgct
     8101 ccccctcggg gtcgtgaacc gtcgtggagg ccgcgcagcc ggtttcgcca tgtcgacggc
     8161 catcgtcgtc ggctactacc tcctttacgc ctcgggagag tcctgggcaa tccgcgggag
     8221 cacgacgcct ttcctcgcga tgtggcttcc gaacattctc ctcgtcgccc tcgggctctg
     8281 gggagtctcg agagtccgac gcgaccgccc gttcctcgac gaacgtgccg gggctgccct
     8341 cacggccgcc gtggcccgag cccttcctcg cgcctggagg cccggaagtc gcgacgggaa
     8401 ggccccggag aacgccgcca ggccgtactc gggagcggtc ggcgcaagct cctggctgat
     8461 cgaccgatac gtcgcaggac gattcctaag agtcctcgcc ctcgttctca cgtcgatcct
     8521 cctcctgtac ctcctcttcg agtacctcga gatctccgac gagatcgcgc gggtgaaccc
     8581 tccgctctcc gcgatcacgg gctacctccg ggcgaagctc gcgccgatcc tcgtcgacgt
     8641 cgtcccgatc gcgttcgcgg cggcggcgct gatcgcgatc gcaggcctcg tccggtctgc
     8701 cgagacgacg gccctcctct cgagcggcgt ctcgctcttc cgcacgaccg cttccgtcct
     8761 cgttctcgcc gcactcgcgg gcgcgggggc ctacctcttc gccgagcagg tcgtcccaag
     8821 ctctgcggcc gaggccgacc ggttcaagaa gatcctggtc ggaaagcccc cggcggccgc
     8881 aaccgacgcc tggcgctcct tcctgaaagg cgattccggc cgcttcttct cggccgagac
     8941 ctgggacccg ggctcctcgt ccctgacggg cgtcacactc ttccgaatcg acccgtccac
     9001 gtaccggctc ctcggcaagt cgcacgcgcc tgaggcccgg atcgtacccg gcaagggaat
     9061 tctcctttca ggcggatgga cgcggacctt cgcggaggac ggctcttccc tcttcctccg
     9121 gcactccggc ccctcgttcg tcgatgcgcc cgaggccgcg cgagccttcc tggccggccg
     9181 ggccgaccct cggcagatga acagcgccga gctggcccgc ttcattcgca tccggcgggc
     9241 cgcaggcgcc gacgtctccg cgatcgagac cggtctctac cagaagagct ccattgccct
     9301 cgcaccgctc ctactgactc tcgtcggact ccccttcgcg ttccgctacg ggcggcgggg
     9361 cgccgtcgcc gggatcggga tcgccctgct cctcggcctc gtctacctgc tcctgggcgc
     9421 ggccctgacc acgggaggag agacgggctc cctcccgccg ctcctcgcgg cgtggggaac
     9481 caacgccttc ttctcgctcg gggcgggctg gggcctgctc ggcgtccgga cctgagcagc
     9541 cccaggccga tcaggcgctc gcggggacgg cgatccccgc ggccttcgcc aggggctcgg
     9601 cgagatcggt ccgctcccag gtgaactcgg caagccggcg cccgaagtgg ccgtacgacg
     9661 cggtcttccg gtagatcggc cgcttcaggt cgagcatctc gatgatcccc ttcggcgtga
     9721 gcctgaagtg atcccggatg agaccgggca ggatcgccgg atcgactctc tcggtgccga
     9781 acgtctcgac gaagacggag acgggatcgg ccacgccgat cgcgtaggcg agctgcacct
     9841 cggccctgtc ggcgaggccc gcggccacca cgttcttcgc gatgtggcgc gccatgtagg
     9901 acgccgagcg gtcgacct
//
LOCUS       DAOTEZ010000128         9880 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4841_length_9880_cov_6.446718, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000128 DAOTEZ010000000
VERSION     DAOTEZ010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9880
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4841_length_9880_cov_6.446718"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="P5164_08415"
     CDS             complement(<1..251)
                     /locus_tag="P5164_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HRY43955.1"
                     /translation="MDWLLKTEPSTYSFEDLVREKTTTWDGVTNAQARLHLRSMKKGD
                     RLVVYHTGDVKAAVGRATVGAAPRPDPADPKGTVVDVKAG"
     gene            complement(251..1579)
                     /locus_tag="P5164_08420"
     CDS             complement(251..1579)
                     /locus_tag="P5164_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HRY43956.1"
                     /translation="MARLRALLARAVDLREGEGPALGWASAYFFLLLASYYVIRPLRD
                     EFGVRGSENALSWLFVGTLLGTAALNPLFAELVTRLTRKVFVPVVYRVLLGTLVLFWA
                     LLSFAPDGWRLPVAQAFFIWASVFNLFAVSVFWGFLADLFRPEQGKRLFGFIAVGGTL
                     GAVAGAALTASLAGPLGPTNLILLAAALLEGAVFCVRGLVRHFHVDDSTCAVAPGAEP
                     EGVPPGSGALSGLATVSRSTYLLAISLFLLFFTVSSTFLYFEQARIVKATFTDSAART
                     AWFARIDLWVNLLSAFTQLFLAGRVVRLLGVGGTLVALPVLTLAGFAALAVSPTAGIL
                     ALVMVTRRAGNFALFRPAREVLFTVLPREEKYAAKSFVDTFVYRFGDVVGAFADKGLR
                     ALGAVGATLAGLFLPVAILWALLSAWLGRRQGKLAAAREGGGTPADIIAA"
     gene            complement(1581..2714)
                     /locus_tag="P5164_08425"
     CDS             complement(1581..2714)
                     /locus_tag="P5164_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRY43957.1"
                     /translation="MARIARGTTRRDFLRAGAAAGAAFGLAGTALDSLGAKTPAPRKL
                     LILGGTAFLGPELVEEAKRAGWTVTLFNRGKTNPHLFPDVEKLRGDRRSDLKALEGRS
                     WDAVVDTSGYFPKDVAASARLLSKSVGLYVFVSTISVYGDTSKPGIDERAPVAKTDQP
                     DADKVSGANYGALKALCEEAAEREMPGKVLVVRPGLIIGPGDTSDRFTYWPVRVARGG
                     EVLAPGTPDDPVQFVDVRDLASWTVAMIGRRRTGVFNATGPKEPLTFGPMLAACREAS
                     GAEATFTWVDAKTLEELKIAPWSDMPCWVPPVGETAGSRRVNNGKAVGEGLTFRPIVE
                     TARDTLAWWRTLPEERRARLKAGLTPEREAAALAAVREKKAKG"
     gene            complement(2816..3967)
                     /locus_tag="P5164_08430"
     CDS             complement(2816..3967)
                     /locus_tag="P5164_08430"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY43958.1"
                     /translation="MTFAGYVLRHLRARPLRATLTAGAFAFSIGLLGFLMLLSSALRK
                     EWSPHMAQRMIVTAKSSFFDKLPMSYMAKIEATPGVDTVVPFDFVVGFHRDARPENQV
                     PIQSAPPEPMLRVYMEAAVPAEQAKAWIEDPTGALVGPLLAEKLGWKVGDRIVLKAPV
                     PGGVVETTIRAVMGYRLDAGLYLHRRYFSQLTADEGRTNMFWVLAKSRGDVDRVTAAL
                     SRALENVPVPVLAMTERQWQLQFLEMLGNVNALLGSVGVATGFALLLITANSLAMSAR
                     ERRNESAVLRVLGFPRGAIAGFLLGEALVYGLAGALFGSAVMWAFARGIGAALDKTQY
                     AGMGQLLVPDAPALLFAAAASLLLALLAGLVPALNLARRSAVELLREAG"
     gene            4058..4465
                     /locus_tag="P5164_08435"
     CDS             4058..4465
                     /locus_tag="P5164_08435"
                     /inference="COORDINATES: protein motif:HMM:NF022591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HRY43959.1"
                     /translation="MRRVLLLPLLAAPALLAAPPAADVSPLRVTELSVCREIVDRGCE
                     SEGRTFGPDVESVAVLTRVEGATGEAFVFHVWSFEAKEVQRVRLPVRAAVYRTWSKKG
                     VKGQPGRWRVEVLDPIGRSLGVVDFTVLPPKID"
     gene            4540..4614
                     /locus_tag="P5164_08440"
     tRNA            4540..4614
                     /locus_tag="P5164_08440"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4572..4574,aa:Val,seq:tac)
     gene            complement(4622..5107)
                     /locus_tag="P5164_08445"
     CDS             complement(4622..5107)
                     /locus_tag="P5164_08445"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRY43960.1"
                     /translation="MVSETYLPPARLLASSRWAALAVVVDGAPAAAMTSYALAPDGAA
                     LYVHLSQMALHTRALLAEPRAALVVSTPDAGDGDPQTLPRLSLSGVALALVPGTPEYE
                     TGKAAYVARFPGAEERFALADFVLFRFETSEARWVGGFARALRMSGAQLAEAIAEAGP
                     A"
     gene            complement(5118..6050)
                     /locus_tag="P5164_08450"
     CDS             complement(5118..6050)
                     /locus_tag="P5164_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY43961.1"
                     /translation="MRIVVTGAAGFLGSHLSDRLLSEGHEVVGIDNLITGHVRNIAHL
                     MGNRAFRFLHHDVTEYIYLDGTVDAVLHFASPASPIDYLQIPIQTLKVGALGTHKALG
                     LSHAKKARFLLASTSEVYGDPLVHPQPESYWGNVNPVGPRGCYDEAKRFAEAMTVAYR
                     NFHGVETRIVRIFNTYGPRMRANDGRIVPSLINQALAGEPLTVFGDGSQTRSFCFVSD
                     LVDGIYRLLLSDQSDPCNVGNPAEMTVMQFAETIRRLTGTRSTIVHKPLPVDDPKQRR
                     PDISLAKSKLGWEPKVELEEGLAATIDYFRALRG"
     gene            6132..6641
                     /locus_tag="P5164_08455"
     CDS             6132..6641
                     /locus_tag="P5164_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43962.1"
                     /translation="MPGLGEDGMRVASLRSLVGFLVLVFAAAGPLAAEPPTLEGIWLF
                     DAKASKNVPEALKGVDLKIDVKDPQVTITKLVGERPVGEPMVVMLDGKSRPQDVGGQR
                     ATVTAKWLEQGRRFEHVVSMLQAGSVFATTQTIVTEVSETGGTMMRKYSIRQARETQE
                     RALVYRRKP"
     gene            complement(6741..8039)
                     /locus_tag="P5164_08460"
     CDS             complement(6741..8039)
                     /locus_tag="P5164_08460"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013910275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRY43963.1"
                     /translation="MNICMVGTGYVGLVTGACLADFGMDVTCVDKDETKIALLRKGVS
                     PIYEPGLEELIHKNAQAKRLHFATEIREAVERALVIFIAVGTPPREDGSPDLSFIFQV
                     AETISEHMNGYKVVVTKSTVPTGTGAQIEEILRKNPGGHKFSVVSNPEFLREGSAIED
                     FMRPDRIVVGSRDAEAIAIVKDVYSPLIVAGVPFVVSDVESAELIKYASNGFLALKIS
                     FINEIAAMCERMGADVKDVARGMGLDKRISPHFLLPGPGFGGSCFPKDASGVVDVARK
                     HGYTFEIMETVLRVNATVKARMVEKVEKVCGELAGKRIAILGLAFKPETDDIRDSSSL
                     KLIEDLVARGASVAAYDPAAMENTKAVLPGIDYADDVYACAAGADALVLATDWNQFRK
                     LDLARLEATMKAKRFVDLRNLYEPREMKRLGWEYVAIGRA"
     gene            complement(8116..9159)
                     /locus_tag="P5164_08465"
     CDS             complement(8116..9159)
                     /locus_tag="P5164_08465"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY43964.1"
                     /translation="MAFRVLVAGGAGLVGSAVARQLLEEGAEVAVVDALVAEGDGRAV
                     REARLEALRPYPNAKVVTADLSLPGAAAEVFRDVRPGAVVNAALFPPGGPGLTAILEA
                     ARAHESPFLVHLSDAALYGPPGAPGQPALEAEPIDPGRDPVLLQRMVEESRLRGSDLP
                     WVILRLFGLLAPGAPPSRFPQNALESLLEEEEVVLPSDEPRDLLHISDAVHGILAALA
                     LRPAGRTINLGSGRGAAPSLVLRLLGDRAHRPVRFRLAGEAPRPVRVADLALAGELLR
                     FRPRTTLETSLDEIVSARLASAETTTSRPRPAARPLRPAAAAPPSPDAPREVSRRELF
                     GFFRGPLGGGRSR"
     gene            complement(9162..>9880)
                     /locus_tag="P5164_08470"
     CDS             complement(9162..>9880)
                     /locus_tag="P5164_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43965.1"
                     /translation="ILGRVEKGEGFLGEMTSASPEGKELSKSLRQTIAATNALLADVR
                     AGKGLVGRLVADEELGDEVAAELAASAASLRKLLAAVERGAGDGEGLVPALLGDPEGK
                     KKFFAMVDSLNATAEGLSGFSRELSSGSGLLSKLVRDEAFAKELTEDLRRISSSLASV
                     AEKVDRGEGTAARIVNDPALFEAIDDILVGVNESKVLRWLVRNRQRSGIQARYHEATA
                     KGTPPAPETLGPAPTPEPRE"
BASE COUNT         1437 a         3300 c         3798 g         1345 t
ORIGIN      
        1 ccggccttca cgtcgacgac cgttcccttc gggtcggccg ggtcggggcg cggcgccgcg
       61 ccgacggtgg cccggccgac ggcggccttc acgtcgcccg tgtggtagac gacgagccgg
      121 tcccccttct tcatcgagcg gaggtggagg cgcgcctgcg cgttcgtgac gccgtcccag
      181 gtggtcgtct tctcacggac gaggtcctcg aacgagtagg tcgacggttc ggtcttcagg
      241 agccagtcca tcaggcggcg atgatatccg cgggcgtccc gccgccttcc cgcgccgcgg
      301 cgagcttccc ctggcgccgc ccgagccacg ccgagaggag cgcccagagg atcgcgaccg
      361 ggaggaagag ccccgcgagc gtcgcgccga cggcgccgag cgcgcggagc cccttgtcgg
      421 cgaacgcgcc gacgacgtcg ccgaagcggt agacgaacgt gtcgacgaag ctcttcgcgg
      481 cgtacttctc ctcgcgcggg aggacggtga agaggacctc gcgcgccggg cggaagaggg
      541 cgaagttgcc ggcccgccgc gtcaccatga cgagggcgag gatccccgcc gtcggggaga
      601 cggcgagcgc cgcgaagccg gcgagcgtga ggaccggcag ggcgacgagc gtcccgccga
      661 cgccgagcag gcggacgacg cggccggcca ggaagagctg cgtgaaggcc gagaggaggt
      721 tcacccagag gtcgatccgg gcgaaccagg cggtccgcgc cgcggagtcg gtgaacgtcg
      781 ctttcacgat ccgcgcctgc tcgaagtaga ggaacgtcga gctgacggtg aagaagagga
      841 ggaagaggga gatcgcgagg aggtacgtcg agcgcgagac ggtcgcgagg cccgagaggg
      901 cgccgctccc cggcgggacc ccctccggct cggctcccgg cgcgacggcg cacgtggagt
      961 cgtcgacgtg gaagtggcgg acgaggccgc ggacgcagaa gaccgcccct tcgaggaggg
     1021 cggccgcgag gaggatcagg ttcgtcgggc cgagcggccc ggcgagcgag gccgtcagcg
     1081 cggctccggc gacggcgccg agcgttccgc cgacggcgat gaagccgaag aggcgcttcc
     1141 cctgctcggg tctgaagagg tcggcgagga agccccagaa gaccgagacg gcgaagaggt
     1201 tgaagacgct cgcccagatg aagaaggcct gggcgacggg gagacgccac ccatcgggcg
     1261 cgaacgagag gagcgcccag aagaggacga gcgtcccgag gaggacccgg tagacgacgg
     1321 ggacgaagac cttccgcgtc agtcgcgtga cgagctccgc gaagagcggg ttcagggcgg
     1381 ccgtgccgag gagcgtcccg acgaagagcc aggagagcgc gttctcgctc ccgcggaccc
     1441 cgaactcgtc gcggagcgga cggatgacgt agtagctcgc gaggaggagg aagaagtagg
     1501 cgctcgccca gccgagggcc ggcccctcgc cctcgcgcag gtcgacggcg cgcgcgagga
     1561 gggcgcggag gcgcgccacg tcagcccttc gccttcttct cgcgaacggc ggcgagggcg
     1621 gcggcttcgc gttccggcgt caggccggcc ttcagccgcg cgcgccgctc ctcggggagc
     1681 gtcctccacc aggcgagggt gtcgcgcgcg gtctccacga tcgggcggaa cgtgagccct
     1741 tctccgacgg cctttccgtt gttcacgcgg cgcgacccgg cggtctcgcc gacgggaggg
     1801 acccagcagg gcatgtcgct ccagggggcg atcttcagct cctcgagcgt cttcgcgtcg
     1861 acccaggtga acgtcgcttc cgcgcccgag gcctcacggc aggcggcgag catcggcccg
     1921 aacgtcaggg gttccttcgg tccggtggcg ttgaagacgc cggtccgccg cctgccgatc
     1981 atcgcgaccg tccacgaggc gaggtcgcgg acgtcgacga actggaccgg gtcgtcgggc
     2041 gtgcccggcg cgaggacctc gccgccgcgg gcgacgcgga cgggccagta ggtgaagcgg
     2101 tcgctcgtgt cgccggggcc gatgatgagg ccgggccgga cgaccaggac cttgcccggc
     2161 atctctcgct cggcggcctc ctcgcagagc gccttgagag cgccgtagtt cgcaccggac
     2221 accttgtcgg cgtcgggctg gtcggtcttc gcgacggggg cgcgctcgtc gatccccggc
     2281 ttcgaggtgt cgccgtagac cgagatcgtc gagacgaaga cgtagaggcc gacgctcttc
     2341 gagaggagcc tcgccgaagc cgccacgtcc ttcggaaagt agccggacgt gtcgacgacg
     2401 gcgtcccagg aacgcccctc gagcgccttc aggtcgctcc ggcggtcgcc gcggagcttc
     2461 tcgacgtcgg gaaagaggtg cgggttcgtt ttcccgcggt tgaagagggt gacggtccag
     2521 ccggcgcgct tcgcctcctc gacgagctcg gggccgagga aggccgttcc gccgaggatc
     2581 aggagcttcc tcggggccgg agtcttcgcc ccgagagagt cgagggccgt gccggccagg
     2641 ccgaatgccg cgcccgccgc ggcgccggct ctgaggaagt ccctccgcgt cgttccccgg
     2701 gcgattcgag ccatctcact cctcctcgtc ggacgcgccg cgtgcggacc gtcgcgggtg
     2761 cggcgccgag tcggaggtac ggcccgcggc gggagcgggt ttccggccgg tcgggtcagc
     2821 ccgcctcgcg gaggagctcg acggcggagc ggcgcgcgag gttcagcgcc ggcacgaggc
     2881 cggcgaggag cgcgaggagg agcgaggcgg ccgcggcgaa gaggagcgcg ggggcgtcgg
     2941 ggacgaggag ctgccccatg ccggcgtact gcgtcttgtc gagcgcggcg ccgatgccgc
     3001 gcgcgaaggc ccacatgacg gcgcttccga agagcgctcc ggcgagaccg tagacgagcg
     3061 cctcgccgag gaggaagccc gcgatcgctc ccctcgggaa accgaggacg cggaggacgg
     3121 cggactcgtt ccgccgctct cgggcgctca tcgcgaggct gttggcggtg atgaggagga
     3181 gggcgaagcc ggtggcgacc ccgacgcttc cgagcagggc gttcacgttc ccgagcatct
     3241 cgaggaactg gagctgccac tgccgctccg tcatcgcgag gaccggcacg gggacgttct
     3301 cgagcgcgcg ggagagggct gcggtcaccc ggtcgacgtc accgcggctc ttcgcgagga
     3361 cccagaacat gttcgtccgc ccctcgtcgg cggtgagctg cgagaagtac cgccggtgga
     3421 ggtagagccc ggcgtcgagg cgatagccca tgacggcgcg gatcgtcgtc tcgacgaccc
     3481 cgccggggac cggcgctttc aggacgatcc ggtcgcccac cttccagccg agcttctcgg
     3541 cgaggagcgg cccgacgagc gcgcccgtcg ggtcctcgat ccaggccttc gcctgctcgg
     3601 ccggcaccgc ggcctccatg tagacgcgga gcatcggctc gggcggggcc gactggatcg
     3661 ggacctggtt ctcggggcgg gcgtcccggt ggaagccgac gacgaagtcg aacgggacga
     3721 cggtgtcgac cccgggagtc gcctcgatct tcgccatgta ggacatcggg agcttgtcga
     3781 agaaggagct cttcgccgtg acgatcatcc gctgcgccat gtggggcgac cactccttcc
     3841 ggagagccga cgacaggagc atcaggaagc cgaggaggcc gatcgagaac gcgaaggccc
     3901 cggccgtcag cgtggcgcgc aggggcctcg cgcggaggtg gcgaaggacg tagccggcga
     3961 aggtcaatgg gagagtctac gtcgggccgg cggccgggtt tccgccccgg ggacgggcac
     4021 ggggcctccg tccgctatca tgaccgaccg cagtccgatg cgtcgcgttc ttctcctccc
     4081 tcttctcgcc gccccggcgc tcctcgcggc cccgcctgcg gccgacgtct ctccgcttcg
     4141 cgtcacggag ctgagcgtct gccgggagat cgtcgaccgg gggtgcgaga gcgaggggcg
     4201 gacgttcggc cccgacgtgg agagcgtcgc cgtcctgacg agggtggaag gcgcgaccgg
     4261 cgaggccttc gtcttccacg tctggtcctt cgaggcgaag gaggtccagc gcgtccgcct
     4321 cccggtgcgg gcagccgtct accggacctg gtcgaagaag ggcgtgaagg ggcagcccgg
     4381 acgctggcgg gtcgaggtcc tcgacccgat cgggcgttcc ctcggggtcg tcgacttcac
     4441 cgtcctgccg ccgaagatcg actgaagggg ggccttccgg ggagcctcgc ccgggttcct
     4501 tgacgcccgc ggcgcccgcc ggctaccgtg ccggaggacg ggaggctagc tcagcggcag
     4561 agcgcctgct ttacacgcag gtggtcgggg gttcgatccc ctcgcctccc accacgacgt
     4621 ctcaggccgg tcccgcctcc gcgatcgcct ccgcgagctg cgctcccgac atccggagcg
     4681 cgcgcgcgaa gccgccgacc catcgggcct cggaggtctc gaagcggaag aggacgaaat
     4741 cggcgagggc gaaacgctct tcggcgccgg ggaaccgggc gacgtaggcc gcctttcccg
     4801 tctcgtactc cggcgtcccg gggacgagcg cgagggcgac gcccgagagc gagagcctcg
     4861 ggagggtctg cgggtcgccg tcgcccgcgt cgggagtcga gacgaccagc gcggcgcgag
     4921 gctcggccag gagggcccgg gtgtggagcg ccatctggga gaggtggacg tagagcgccg
     4981 cgccgtccgg ggcgagggcg taggaagtca tcgcggcggc gggggctccg tcgacgacga
     5041 cggcgagggc cgcccagcgg gaggaggcga ggaggcgggc cggcgggagg tacgtctcgg
     5101 aaaccatcgg gcggtcctca gccgcgcagc gcgcggaagt agtcgatcgt ggcggcgagg
     5161 ccttcctcga gctcgacctt cggctcccac ccgagcttcg acttcgcgag cgagatgtcg
     5221 gggcggcgct gcttcgggtc gtcgaccggg agcggcttgt ggacgatcgt ggagcgggtg
     5281 cccgtgagcc gccggatcgt ctcggcgaac tgcatgaccg tcatctcggc cgggttcccg
     5341 acgttgcacg ggtcggactg gtccgagagg aggagccggt agatgccgtc gacgaggtcg
     5401 gagacgaaac agaaggagcg ggtctgggag ccgtctccga agaccgtgag gggctcgccc
     5461 gcgagcgcct ggttgatgag cgacgggacg atccgtccgt cgttcgcccg catcctcggt
     5521 ccgtacgtgt tgaagatccg cacgatgcgc gtctcgacgc cgtggaagtt ccggtaggcg
     5581 acggtcatcg cctccgcgaa tcgcttcgcc tcgtcgtagc agccgcgcgg cccgacgggg
     5641 ttcacgttgc cccagtagct ctcgggctgc gggtgtacga gcgggtcgcc gtagacctcg
     5701 gacgtggagg cgaggaggaa ccgggccttc ttcgcgtggg agaggccgag cgccttgtgg
     5761 gtgccgaggg cgcccacctt cagagtctgg atcgggatct ggaggtagtc gatcggcgag
     5821 gcgggcgagg cgaagtggag gacggcgtcg accgtcccgt cgaggtagat gtactccgtg
     5881 acgtcgtggt ggaggaatcg aaacgcccgg ttccccatca ggtgggcgat gttcctcacg
     5941 tggccggtga tcaggttgtc gatcccgacg acctcgtgcc cttcggagag gaggcggtcg
     6001 gagaggtggc tcccgaggaa tccggcggcg ccggtcacga cgatgcgcat gcaacccgac
     6061 attctacggg atcgaccgaa agggagacgg aaacccgcga ggagcacaat agagcgttcg
     6121 gaggagcccc gatgccgggg ctcggggagg acggcatgag agtggcttcg ctccgctcgc
     6181 tcgtcggttt cctggtcctg gtcttcgccg cggccggccc gctcgcggcg gagccgccaa
     6241 ccctcgaggg gatctggctc ttcgacgcca aggcgtcgaa gaacgtcccc gaggcgttga
     6301 aaggcgtcga cctgaagatc gacgtgaagg acccccaggt cacgatcacg aagctcgtcg
     6361 gggagcgtcc cgtcggcgag ccgatggtcg tgatgctgga cgggaagtcc cgcccgcagg
     6421 acgtcggcgg ccagcgcgcc acggtcaccg cgaagtggct cgagcagggg cgccggttcg
     6481 agcacgtcgt cagcatgctg caggcgggaa gcgtcttcgc gacgacgcag acgatcgtga
     6541 ccgaggtctc ggagaccggc ggcacgatga tgcggaagta ctcgatccgc caggcgcgcg
     6601 agacgcagga gcgcgcgctg gtctaccgcc gcaagccctg accccgtcgg ggtcgggatt
     6661 cccggacccg gggccggcgc gccgcggcgg ccccgggtgt cggcgtggcc tcgttcgggg
     6721 gggggctccg gccgggccgc tcaggcccgc ccgatggcca cgtactccca gccgaggcgc
     6781 ttcatctccc gcggctcgta gaggttccgg aggtcgacga agcgcttcgc cttcatcgtc
     6841 gcctcgaggc gcgcgaggtc gagcttgcgg aactggttcc agtcggtggc caggacgagg
     6901 gcgtcggctc ccgccgcgca cgcgtagacg tcgtcggcgt agtcgatgcc cggcaggacg
     6961 gccttcgtgt tctccatcgc tgccgggtca taggccgcga cggaggcgcc gcgggcgacg
     7021 aggtcctcga tcagcttgag ggacgagctg tcgcggatgt cgtcggtctc cggcttgaac
     7081 gcgaggccga ggatggcgat ccgcttcccg gcgagctccc cgcagacctt ctcgaccttc
     7141 tcgaccatcc gggccttcac cgtcgcgttc acgcggagga cggtctccat gatctcgaac
     7201 gtgtagccgt gcttgcgggc gacgtcgacg acgccggagg cgtccttcgg gaagcaggag
     7261 ccgccgaagc ccggcccggg gaggaggaag tggggggaga tccgcttgtc gaggcccatc
     7321 ccccgggcga cgtccttcac gtcggccccc atccgctcgc acatcgcggc gatctcgttg
     7381 atgaaggaga tcttcagcgc gaggaagccg ttcgaggcgt acttgatcag ctcggccgac
     7441 tcgacgtcgg agacgacgaa cgggacgccc gcgacgatga gcggcgagta gacgtccttg
     7501 acgatcgcga tcgcctcggc gtcgcgcgag ccgacgacga tgcggtcggg gcgcatgaag
     7561 tcctcgatcg cggacccctc ccggaggaac tcggggttcg agacgaccga gaacttgtgc
     7621 ccgccggggt tcttccggag gatctcctcg atctgcgccc ccgtgccggt cgggacggtc
     7681 gacttcgtga cgacgacctt gtacccgttc atgtgctccg agatcgtctc ggcgacctgg
     7741 aagatgaacg agaggtcggg cgagccgtcc tcgcggggcg gggtgccgac cgcgatgaag
     7801 atgacgagcg cccgctcgac cgcttcgcgg atctccgtcg cgaagtggag ccgcttggcc
     7861 tgggcgttct tgtggatcag ctcctcgagg cccggctcgt agatcggcga gacgcccttc
     7921 cggaggaggg cgatcttcgt ctcgtccttg tcgacgcagg tgacgtccat cccgaagtcg
     7981 gcgaggcagg cgcccgtcac gaggcccacg tatcccgttc cgaccatgca gatgttcatt
     8041 cgttcctcca gaaacggact cccgcggggc ggtgtccagc cggaggagtc tagcgtcggc
     8101 ggggcgggtc tgtcttcagc gggagcgccc gccgccgagc gggccccgga agaagccgaa
     8161 gagctcccgc cgggagacct cccggggcgc gtccggcgag ggcggggccg cggcggcggg
     8221 gcggagcggc cgggccgcgg ggcgcggccg gctcgtcgtc gtctcggcgg acgccagccg
     8281 ggcggagacg atctcgtcga gcgacgtctc gagcgtcgtc cggggccgga agcggaggag
     8341 ctcgccggcg agcgccaggt cggcgacccg gacggggcgc ggggcctctc ctgcgagacg
     8401 gaagcggacc gggcggtgcg cgcggtcgcc caggaggcgg aggacgagcg agggggcggc
     8461 cccacgcccg ctcccgaggt tgatcgtgcg ccccgccggg cggagggcga gcgcggccag
     8521 gatgccgtgc accgcatcgg agatgtggag gagatcgcgc ggctcgtcgg aggggaggac
     8581 gacctcctct tcctcgagga gggactcgag ggcgttctgc gggaaacggc tcggcggcgc
     8641 gccgggggcg agcaggccga agagacgcag aatgacccag gggaggtcgg agcctcgcag
     8701 gcgcgactcc tcgaccatgc gctgcaggag gacgggatcg cgcccggggt ctatcggctc
     8761 ggcctcgagc gcgggctggc ccggtgcgcc cggggggccg tagagagcgg cgtcggagag
     8821 gtggacgagg aacgggctct cgtgcgcgcg cgccgcctcg agaatcgccg tcaggcccgg
     8881 cccgccgggc gggaagagcg ccgcgttcac gacggccccg ggccggacgt cgcggaagac
     8941 ctcggccgcg gcgccgggga gcgagaggtc ggccgtgacg accttcgcgt tcgggtaggg
     9001 gcggagcgcc tcgagccggg cctcccggac ggcccggccg tccccctccg cgacgagggc
     9061 gtcgacgacg gcgacctcgg ccccctcctc gaggagctgc cgagcgacgg ccgagccgac
     9121 gaggccggcg ccgccggcga cgaggacgcg gaaggccatc gctactccct cggttcgggc
     9181 gtcggcgccg ggccgagcgt ctcgggcgcg gggggcgtac ccttcgccgt cgcttcgtgg
     9241 tagcgggcct ggatccccga gcgctgccgg ttccgcacga gccaccggag gaccttcgac
     9301 tcgttcacgc cgacgaggat gtcgtcgatc gcctcgaaga gggccgggtc gttcacgatc
     9361 cgcgcggccg tcccctcgcc ccggtcgacc ttctcggcga cgctcgcgag gctcgaggag
     9421 atccgccgca ggtcctcggt cagctccttc gcgaaggcct cgtcgcggac gagcttcgac
     9481 aggaggccgg agccggagga gagctcccgc gagaagccgg agagcccctc cgccgtcgcg
     9541 ttcagcgagt cgaccatcgc gaagaacttc ttcttccctt ccgggtcgcc gaggagcgcg
     9601 gggacgaggc cctcgccgtc gcccgcaccc ctctcgacgg cggcgaggag cttccggagc
     9661 gaggcggcgg aggccgcgag ctcggcggcg acctcgtctc cgagctcttc gtcggcgacg
     9721 agccttccga cgagcccctt gccggcccgt acgtcggcca gcagggcgtt cgtcgcggcg
     9781 atcgtctgcc ggaggctttt agagagctcc ttgccctccg gcgacgcgct cgtcatctcg
     9841 ccgaggaacc cctcgccctt ctcgacgcgc ccgaggatcg
//
LOCUS       DAOTEZ010000129         9866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4852_length_9866_cov_5.259607, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000129 DAOTEZ010000000
VERSION     DAOTEZ010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9866
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4852_length_9866_cov_5.259607"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="P5164_08475"
     CDS             complement(<1..877)
                     /locus_tag="P5164_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43966.1"
                     /translation="MPRRFALALLALLILLLLGLLAGRHVQESRQKRQVAARPTPTRT
                     IPPPPTPIPARLVSIWFESADDGLFHPEAREVPAAVDDVAFLRSLATAVLEGPRHPAL
                     LKPFPDGWTLRAAFRLADGIAVLDLAPPAPPTPAPTPEPPPVEAEGKKGAKQPTPTST
                     IPPPVPIAEPPKRWETGSSEEVGAAQSLVLTVTKNVHAVSRVILLVGGEPVETLAGHV
                     DLTHPLLPDLSRAADEAPLVAPMPEPEATSDPAVTSSSAVPSAVPPAAPTSAVSPAPA
                     PPQVPPIVAPRPAARP"
     gene            complement(888..2441)
                     /locus_tag="P5164_08480"
     CDS             complement(888..2441)
                     /locus_tag="P5164_08480"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRY43967.1"
                     /translation="MTRPALLPCLALAALLALVPVRAADPTATFVAPGGSSLLVAAAD
                     AEGKTWLSVRDVATALGGTLAHEAATGSFELKIGPHTAVFGTGTPIAVVDTRLVPLAA
                     AVRAEAGTAYAEPDFFSRVLSPLFGVAFTWDRASRTLAARPVETPEIGVEATVSAFEG
                     TTKVVFRFSQAPSYRTEKGADQVVLRFPGAKLVPATPEVVPDDERVARLLLRPGELAV
                     VLKGKDLSTNVYPLAAPPRIVVDVTRGAPPAPAAAPAATRVPGALVLPTPTPTPRPEP
                     RVVVIDPGHGGTEEGAKGPGGLLEKDVVLALSRTVREVLTARGYRVVTTRDSDASVGF
                     QDRTATANEAKADVFLSLHMNASRSPSAHGTEVYYLSLDASDRAAAALADAENEDEPK
                     PTPSAETNAALRDLDLILWDLAQNQHLTASSRLAEIIQADFNRLLGITTRGVKQAPFR
                     VLIGVNAPAVLVEVAFISNPEEEQKMASEEFRKAVAETLAGSLDNFFRAAASGAAPVP
                     AALPPSGSR"
     gene            2549..3013
                     /locus_tag="P5164_08485"
     CDS             2549..3013
                     /locus_tag="P5164_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43968.1"
                     /translation="MHDTIPRRTTTPCAATVQLDDGSLLDVTFWLLPDPHRERGVTPM
                     RVLLEEDRRFLPIGLAGGGSCLLSRDALVTVSIEAEGPGGEPSATEGAALDLVTLQLV
                     SGAEVSGVLTTTALEGHGRMSDVFNLAGRFVPVGVGSQLVFVATSRIARVSF"
     gene            3027..4835
                     /locus_tag="P5164_08490"
     CDS             3027..4835
                     /locus_tag="P5164_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002620768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HRY43969.1"
                     /translation="MASRVDLLVANLLQTTGDALYIVPGEKLFMTRGDVRAVVGREPV
                     GVDSFRAVVAELVPDSTPEELAVARHRMPWPTGPGKDEVEIRFGQVGTMPAVMLCRPS
                     RMSGSAIRRPKHGAPPVSSIGVPEAPPSVGMLESTPPVPEAIPVPPELAAPSAAFPGA
                     PAPAPAVAAPAPVPAPAPVPAPRPAAPEEIPYPAGGALEAPSLRAAGPAGRPLDALLL
                     RMLERKASDVHLSSGTRPVFRVHGEMAIQEDQPSLASDAVEKLLGPIFPEKNRTEFME
                     RHDTDFAYEIPGVARFRVNVFRDRHGVGAVFRQIPIEILTAKQLNLPDAVVRLGDLSK
                     GLVLVTGPTGSGKSTTLAAIVDYVNETRTDHIITIEDPVEFVHRNKKCLVNQREIGSH
                     TRSFKDALRAALREDPDIVLVGEMRDLETIAIAIETAETGHLVFGTLHTTTAMSTVDR
                     IVDQFPADRQQQIRVMLADSLKGVVAQTLLRKIGGGRVAALEILLTNNAVANLIRDGK
                     TFQLPSVLQTSKAQGMRLLNDSLLDLVKQGLVEPREAYLKAIDKSALLGAFKSNNIPL
                     PTETAAEAPPAAPAPAPAPGGAKPGGAPAPARRPGS"
     gene            complement(4867..5496)
                     /locus_tag="P5164_08495"
     CDS             complement(4867..5496)
                     /locus_tag="P5164_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43970.1"
                     /translation="MKRLGFLALSLLVLAAAILPAGCATLDQLASTLVNLRRLEFRIG
                     QVTGFRLAGIDLGRASALSQLTPFDAMALAGAYASRRLPAELVVEVEAVNPNDGTAGT
                     PRTTSTLTSLECRLLVDGRPTVTGNIDRPFEIPGTGQATVVPIRLSLDLYEFFGEKRY
                     AELIDLALALGGESRDTSRISLDALPTVTTPLGNITYPGRITIAGSRLR"
     gene            complement(5616..5978)
                     /gene="ssrA"
                     /locus_tag="P5164_08500"
     tmRNA           complement(5616..5978)
                     /gene="ssrA"
                     /locus_tag="P5164_08500"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(6096..7241)
                     /locus_tag="P5164_08505"
     CDS             complement(6096..7241)
                     /locus_tag="P5164_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HRY43971.1"
                     /translation="MQRAWKRVKKYAYLIVFVLPALVALGVRFGGLASYLTPIVAFVL
                     IPLLDLLVGEDRVNYTPEEEKAMDGARYYRLVTYAFVPVYWAVLAFVVWGASTGGWSL
                     AEYAGNTWSMGILSGIGIVVAHELGHKASRLEKRLAKALLHPTFYGHFTQEHNLGHHL
                     MVATPEDPASSRLGESFWRFYPRTVFGTFRKNLALERQRLARTGKGAWSLSNETWRNV
                     LWPVVLTIAAYLAGAALAPEAPGLLGLGAGASAALVVVVQAVLGFSLLEIVNYLEHYG
                     LERRKLPNGKYERVTPLHSWNSNHLVTNGFLYHLQRHSDHHAWPARRYQTLRNFPEAP
                     QLPTGYAGMIWLAAIPPLWFRVMDRRVLEFREQQARSEAVEGQGIVA"
     gene            complement(7280..7558)
                     /locus_tag="P5164_08510"
     CDS             complement(7280..7558)
                     /locus_tag="P5164_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010639609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRY43972.1"
                     /translation="MTPGNAISEAEASLVSRHGIWILLPEGEFFLPSDQFPWFQQGPV
                     AAVLNVQRLSADHLYWPDLDVDLSVSSIRDPARFPLVARTPTTGLAKG"
     gene            complement(7647..7754)
                     /locus_tag="P5164_08515"
                     /pseudo
     CDS             complement(7647..7754)
                     /locus_tag="P5164_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639677.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
     gene            7874..8212
                     /gene="cutA"
                     /locus_tag="P5164_08520"
     CDS             7874..8212
                     /gene="cutA"
                     /locus_tag="P5164_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HRY43973.1"
                     /translation="MTTPVTLCLTTVGSEEDAARIARHLVETRAAACVSIVSPVRSVY
                     SWRGEIQDEREWLLLIKTARPAAELRDLLHAVHPYEVPELLAFPAGFADEAYARWVAE
                     GSRAKAEPTE"
     gene            8458..9513
                     /locus_tag="P5164_08525"
     CDS             8458..9513
                     /locus_tag="P5164_08525"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HRY43974.1"
                     /translation="MGKSTLLRHALRDAADFVVFDPVVDVEKVRRDPELLLDNHRTPL
                     VLDELQYEPELLPSLKRRIDRDRSPGRDVLTGSQQWGALATVAESLAGRAALLDLDGF
                     SLRESLEATAEKHWLDAALTGQELNRSQLGREIGVSPRSAERWLGTLRATFQWFEVPA
                     FSGNTGKRVASKAKGYVADTGFAGHALAISTPNAVASHPAWGALFETAVYAEIREAVS
                     LISPRPVVHHWRTGAGAEVDLLFERDGTFFPIEVKAASRPSRRDTNGITAFRKTYPKL
                     RVARGLVVAPCETMAPLSENDWAMPWDAVLAPDRDGGESPRVRSRPLIPPRAPAPRPR
                     RGPPSPRSRTRGGRARR"
     gene            complement(9425..>9866)
                     /locus_tag="P5164_08530"
     CDS             complement(9425..>9866)
                     /locus_tag="P5164_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRY43975.1"
                     /translation="DSFLVTRGVKTLALRMRAHDANGRAVAAFLAVHPVVVRVLYPGL
                     PDHPQHALASRQMSGFGGMLSVELGSYAAAKRFLDALRLFTLAESLGGVESLAGHPAS
                     MSHGSLAPEERAARGITDGLVRLSCGIEETEDLVADVERGLGEV"
BASE COUNT         1413 a         3495 c         3614 g         1344 t
ORIGIN      
        1 tggggcgcgc cgcgggacgc ggcgcgacga tggggggaac ctgggggggc gccggagcgg
       61 gagacaccgc cgaagtcggg gccgccggcg gcactgcgga gggaacggcc gaagatgacg
      121 taacggccgg gtcggaggtt gcctccggct cgggcatcgg cgcgacgaga ggcgcctcgt
      181 cggccgcgcg ggagaggtcg ggaaggagcg ggtgggtcag gtcgacgtgt cccgcgagcg
      241 tctccaccgg ctccccgccg acgaggagga tgacccggga aacggcatgg acgttcttcg
      301 tgacggtgag aacgagcgac tgggcggcac cgacctcctc agaggaaccg gtctcccagc
      361 gttttggcgg ctcggcgatc gggaccggcg gcgggatcgt cgacgtgggc gtcggctgct
      421 tcgccccctt ctttccctcc gcctcgacgg gcggcggctc gggggtcggc gccggcgtcg
      481 gcggggcggg cggggcgagg tcgaggacgg cgatgccgtc ggcgaggcgg aaggcggctc
      541 gcagcgtcca accgtcggga aacggcttca ggagggccgg gtgacgcggc ccttcgagga
      601 ccgccgtggc gaggctcctg aggaacgcga cgtcgtcgac cgccgccggg acttccctcg
      661 cttccgggtg gaagaggccg tcgtccgcgg actcgaacca gatcgagacg agccgggccg
      721 ggatcggcgt cgggggcggc gggatggtcc gggtcggcgt gggccgcgcg gccacctgcc
      781 tcttctgcct cgactcctgg acgtgccttc cggcgagaag ccccagcagg aggaggatca
      841 ggagcgcgag aagcgcgaga gcgaagcggc gcggcatcgg gaggacgtca gcgcgacccg
      901 ctcggcggca gggcggccgg aaccggtgcc gcaccgctgg ccgcggcccg gaagaagttg
      961 tcgagcgacc cggcgagcgt ttctgccacg gcctttcgga actcctcgga ggccatcttc
     1021 tgctcctcct cggggttcga gatgaaggcg acctcgacga ggacggccgg cgcgttgacg
     1081 ccgatcagga cgcggaacgg ggcctgcttg acgccgcggg tcgtgatgcc gaggagccgg
     1141 ttgaagtcgg cctggatgat ctcggcgaga cggctcgacg ccgtgaggtg ctggttctgg
     1201 gcgaggtccc agaggatcag gtcgaggtcg cgcagcgccg cgttcgtctc ggccgacggc
     1261 gtcggcttcg gctcgtcctc gttctcggcg tcggcgagcg cagcggcggc gcggtcggag
     1321 gcgtcgaggg agaggtagta gacctccgtg ccgtgggccg acggcgagcg cgaggcgttc
     1381 atgtggagcg agaggaagac gtcggccttc gcctcgttcg cggtggccgt ccggtcctgg
     1441 aatcccaccg acgcgtcgct gtcacgcgtc gtgaccaccc ggtagccgcg agcggtcagc
     1501 acctcgcgga cggtccgcga gagggcgagg acgacgtcct tctcgaggag cccgcccggg
     1561 cccttcgccc cctcctccgt cccgccgtgg cccgggtcga tcacgacgac gcgcggctcg
     1621 gggcggggcg tgggcgtcgg cgtggggagg acgagggcgc cggggacgcg ggtcgccgcg
     1681 ggggcggcgg ccggggccgg cggagcgccg cgcgtgacgt cgacgacgat ccggggcggc
     1741 gccgcgagcg ggtagacgtt cgtggagagg tccttcccct tcaggacgac ggcgagctcg
     1801 cccggccgca ggaggagccg ggccacgcgc tcgtcgtcgg ggacgacctc gggcgtcgcc
     1861 gggacgagct tcgcgcccgg gaaccggagg acgacctggt cggccccctt ctcggtgcgg
     1921 taggacgggg cctgcgagaa gcggaagacg accttcgtcg tcccctcgaa cgcggagacg
     1981 gtcgcctcca cgccgatctc gggcgtctcc accggccgcg cggcgagcgt gcgcgaggcg
     2041 cggtcccacg tgaaggcgac gccgaagaga ggagagagga cccgcgagaa gaagtccggc
     2101 tccgcgtagg ccgtacccgc ctcggccctc accgccgcgg cgagcgggac gaggcgcgtg
     2161 tcgacgacgg cgatcggcgt cccggtgccg aagacggccg tgtgcgggcc gatcttcagc
     2221 tcgaacgagc ctgtcgccgc ctcgtgggcg agcgtgccgc cgagggcagt cgcgacgtcg
     2281 cgcacggaga gccacgtttt cccctcggcg tcggccgccg ccacgaggag cgaggagccc
     2341 ccgggcgcga cgaacgtcgc cgtcgggtcc gcggcgcgca cgggcacgag ggccaggagc
     2401 gccgcgaggg cgaggcacgg gaggagggcg ggccgggtca cgtcccgcga ttatctacgc
     2461 gcccgcgttc ccgccgcggg acgggccgga agcgggcctg cggcccgtcc gattcgccta
     2521 agatcggggc gggtccccgg aggtttgcat gcacgacacc atccctcgca ggacgacgac
     2581 cccgtgcgcc gccaccgtcc agctcgacga cggcagcctg ctcgacgtga cgttctggct
     2641 cctccccgac ccgcaccgcg agcggggcgt gacgccgatg cgcgtcctcc tcgaggagga
     2701 ccgccgcttc ctgccgatcg gcctggccgg cgggggaagc tgcctcctct cgcgcgacgc
     2761 tctcgtcacc gtgtcgatcg aggccgaggg ccccggcggc gagccgtcgg cgacggaagg
     2821 ggccgcgctc gacctcgtca cgctccagct cgtctcgggc gcggaggtct ccggcgtcct
     2881 gacgaccacc gcgctcgagg gacacggccg gatgtccgac gtcttcaacc tcgcggggcg
     2941 gttcgtgccg gtgggggtcg ggtcgcagct cgtcttcgtc gccacctcgc ggatcgcgcg
     3001 ggtctccttc tgaagggacg gcgacgatgg cctcccgagt cgatctcctc gtcgcgaacc
     3061 tcctccagac gaccggcgac gcgctctaca tcgtcccggg cgagaagctc ttcatgacgc
     3121 gcggcgacgt gagggcggtc gtcgggcgcg aaccggtcgg cgtcgactcg ttccgcgccg
     3181 tggttgccga gctcgtcccg gactcgacgc ccgaggagct cgccgtcgcg cggcaccgga
     3241 tgccctggcc gaccggcccc gggaaggacg aggtcgagat ccgcttcggc caggtcggca
     3301 ccatgccggc cgtcatgctc tgccggccca gccggatgtc cggctcggcg atccggcggc
     3361 cgaagcacgg cgcaccgccc gtctcctcga tcggcgtccc ggaggcccct ccctccgtcg
     3421 gaatgctcga atcgactccg cccgtccccg aggcgatccc ggtgccgccg gagctggcgg
     3481 ccccctcggc cgcgttcccc ggggcccccg ctccggcccc cgcggtcgcg gcccccgccc
     3541 cggtcccggc ccccgcgccg gtccccgcgc cgaggcccgc ggcgccggag gagatcccgt
     3601 accccgccgg cggggcgctc gaggcgccct cgctccgcgc cgccggtccc gccggccgcc
     3661 cgctcgacgc gctcctcctc cggatgctcg agaggaaggc ctccgacgtc cacctctcct
     3721 cgggcacccg gcccgtcttc cgggtccacg gggagatggc gatccaggag gatcagccct
     3781 cgctcgcgtc cgacgccgtc gagaagctcc tggggccgat ctttccggag aagaaccgga
     3841 ccgagttcat ggagcggcac gacaccgact tcgcctacga gattccgggc gtcgcccgct
     3901 tccgcgtcaa cgtcttccgc gaccggcacg gcgtgggcgc cgtcttccgc cagatcccga
     3961 tcgagatcct cacggcgaaa cagctgaacc tgcccgacgc ggtcgtgagg ctcggcgacc
     4021 tctcgaaggg gctcgtcctc gtcaccgggc cgaccggctc gggcaagtcg acgacgctcg
     4081 ccgcgatcgt cgactacgtc aacgagacgc gcaccgacca catcatcacg atcgaggacc
     4141 cggtcgagtt cgtccaccgc aacaagaagt gcctcgtgaa ccagcgcgag atcgggagcc
     4201 acacgcgctc gttcaaggac gccctccgcg cggcgctccg cgaggacccc gacatcgtcc
     4261 tcgtcggcga gatgcgcgac ctcgagacga tcgccatcgc catcgagacg gccgagacgg
     4321 ggcacctcgt cttcggcacg ctccacacca cgaccgcgat gtcgacggtc gaccggatcg
     4381 tcgaccagtt ccccgccgac cggcagcagc agatccgggt catgctcgcc gactcgctca
     4441 agggggtcgt cgcgcagacg ctcctccgga agatcggcgg cgggcgcgtc gccgccctcg
     4501 agatcctcct gacgaacaac gccgtcgcga acctcatccg cgacggcaag acgttccagc
     4561 tcccgtcggt cctccagacc tcgaaggcgc aggggatgcg cctcctgaac gactcgctcc
     4621 tcgacctcgt caagcagggg ctcgtcgagc cgcgcgaggc ctacctgaag gcgatcgaca
     4681 agagcgccct cctcggcgcc ttcaagagca acaacatccc gctgccgacg gagaccgcgg
     4741 cggaggcgcc ccccgcggcg cctgcgccag cgccggcccc cggcggagcg aagccgggtg
     4801 gcgctccggc tccggcgagg cggcccggga gctgaaggcg agcggggacg ggaggcgacg
     4861 ccggcgtcag cggagccggc tgccggcgat cgtgatgcgg cccgggtagg tgatgttccc
     4921 gaggggcgtc gtcacggtcg gcagggcgtc gagggagatg cgggacgtgt cgcggctctc
     4981 cccgccgagc gcgagcgcga ggtcgatcag ctccgcgtag cgcttctcgc cgaagaactc
     5041 gtacaggtcg agcgagaggc ggatcggcac gaccgtcgcc tgccccgttc ccgggatctc
     5101 gaagggcctg tcgatgttcc ccgtcacggt gggccggccg tcgacgagga gccggcactc
     5161 caggctcgtg agcgtgctcg tcgtccgcgg cgtccccgcc gttccgtcgt tcgggttcac
     5221 ggcctcgacc tcgacgacga gctcggccgg cagcctccgg gaggcgtacg cccccgcgag
     5281 cgccatggcg tcgaacggcg tgagctgcga gagagccgac gcccgcccga ggtcgatccc
     5341 cgcgagccgg aagcccgtga cctggccgat ccggaactcg agccgcctca ggttcacgag
     5401 ggtcgaggcc agctggtcga gcgtcgcgca gccggcgggg aggatggcgg cggcgaggac
     5461 gaggagggag agcgcgagga agccgagccg cttcatgcgg agatcgtccc acggaggggc
     5521 ggagccgcga tgcacgcggg cggacgggtg aggtcgccct tctcgagcga acggcccgcc
     5581 gccgggttcc cggcggcggg ccgcgactcc cgagatggtg gaggcggggg gagtcgaacc
     5641 cccgtccgaa gcgaccgtac gtcgaggttc tccacgctca gtcccgactt cttttctcgt
     5701 cgccgcgctt ccgggtcggg accaccaggc gctgcgacca acctggaacg tctcggacct
     5761 cgccttcagg tggcgggctt cggtcccagc ctggttctct caacagggcg gcggagcgct
     5821 ccaggctggc tccccgtgcc ccgcccgcgg ttaattaagc cgcgagagcg agttcgctgt
     5881 cgttggcagc ttttatttac cgtcgttttt acgggacaaa cggcatgccc ggcgtgctcc
     5941 tcgatcgcga gatgccccgt cgaaaccggg tcgcccccgt gatcgggctt atcttaccgc
     6001 gggagtcaag gaggcccgtg ggcgttctcg accgtggtcg agagtgctcg cggacatttt
     6061 cgaccatggt cgaaatcacc cgcgcctccc ccccgctacg cgacgatccc ctgcccctcg
     6121 acggcctccg agcgcgcctg ctgctcgcgg aactcgagga ctcggcggtc catcacccga
     6181 aaccagagcg gcggaatcgc ggcgagccag atcatcccgg cgtagcccgt cgggagctgc
     6241 ggcgcctcgg ggaagttgcg gagggtctgg tagcggcgcg ccggccaggc gtggtggtcg
     6301 ctgtgccgct ggaggtggta gaggaagccg ttcgtgacga ggtggttgct gttccacgag
     6361 tggagcggcg tcacccgctc gtactttccg ttcgggagct tccggcgctc gaggccgtag
     6421 tgctcgaggt agttcacgat ctcgaggagc gagaagccga ggacggcctg gacgacgacg
     6481 acgagcgccg ccgaggctcc ggcgccgagg ccgagaagcc ccggcgcctc cggcgcgagc
     6541 gccgcgcccg cgaggtaagc ggcgatcgtc aggacgaccg gccagaggac gttccgccag
     6601 gtctcgttcg agaggctcca cgcccccttc cccgtccgcg cgaggcgctg ccgctcgagc
     6661 gcgaggttct tgcggaaggt gccgaacacc gtccgcgggt agaagcgcca gaacgactcg
     6721 ccgaggcgcg acgaggccgg gtcctccggc gtcgcgacca tcaggtggtg gccgaggttg
     6781 tgctcctgag tgaagtgccc gtagaaggtc gggtgcagga gcgctttcgc gagccgcttc
     6841 tcgagccggc tcgccttgtg cccgagctcg tgcgcgacga cgatgccgat gccggagagg
     6901 atgcccatcg accaggtgtt gccggcgtac tcggcgaggc tccagccgcc cgtgctcgcg
     6961 ccccacacga cgaacgcgag gaccgcccag tagaccggga cgaaggcgta cgtcacgagc
     7021 cggtagtacc gcgcgccgtc catcgccttc tcctcctcgg gcgtgtagtt cacgcggtcc
     7081 tccccgacga ggaggtcgag gagcgggatg aggacgaacg cgacgatcgg tgtcaggtac
     7141 gacgcgaggc cgccgaagcg caccccgagc gcgacgagcg ccggcaggac gaagacgatc
     7201 aggtaggcgt acttcttgac cctcttccag gcgcgctgca cggcgtgacc cccgatcggc
     7261 cccagttcct cggcccggtc taccccttcg cgagcccggt cgtcggtgtc cgcgcgacga
     7321 gcgggaagcg cgccgggtcc cggatcgacg agacggagag gtcgacgtcc agatccggcc
     7381 agtagaggtg atccgccgac aggcgctgga cgttcagcac ggcggccacc ggcccctgct
     7441 gaaaccaggg aaactggtcg gacgggagga agaactcccc ttccggcagg aggatccaga
     7501 ttccgtgcct ggaaacgaga gaggcctcag cttccgaaat ggcgtttcca ggcgtcacgg
     7561 aagacctcct gattctcctc gacgagcctt cgcgcctcgg cgagggactt gtccggcagc
     7621 ccgtggttga cggccggctc gagtcagaac ctggcctcgc cgtccggcga atgaacgtgg
     7681 acgtgcgccc ggttctcctc tcgggagaag aagaagaacc gatacgggcc caccctgaga
     7741 atggtcggac tcatcggcgt cgattctatc ggggcgggac gacagggact gcgagccgcg
     7801 cggagtagat cgggcagagg agcctggtcg gcccccgttc ccgcctcctg cgtgcgctgg
     7861 tagcgttccg cgcatgacga cgcccgtgac gctctgcctg acgaccgtcg gctccgagga
     7921 ggacgccgcc cgcatcgccc gccacctcgt cgaaacgaga gccgccgcct gcgtgtcgat
     7981 cgtgtcgccg gtccgttccg tctactcgtg gaggggcgag atccaggacg agcgggagtg
     8041 gctgctgctc atcaagacgg cccgccccgc cgccgagctc cgcgacctcc tccacgccgt
     8101 ccacccctac gaggtcccgg agcttctcgc cttccccgcg ggcttcgccg acgaggcgta
     8161 tgcgcggtgg gtggcggagg ggagcagggc gaaggccgag cccaccgagt aggcgcggct
     8221 ccccttcccg ctgcagggtc gacggggccc gcaccttcgc ttcccggccc tcctcgcggg
     8281 gccaggccgc gaccgcgacc agaggaaacg cgcccggccc gacggcggcc cgaactcctt
     8341 gtaagaccgg cgcgtcacga tcaaaccgca aggcatgttc ctgccccgaa atgcggagga
     8401 gcgcctctcg gcgctcatga agagcttccc cccgtcgtcg tcagcgttgc gcggcaggtg
     8461 ggcaagagca cgctccttcg acatgctctg cgcgatgcgg ccgacttcgt cgtcttcgac
     8521 cccgtcgtcg acgtggagaa agtccgccgc gacccggagc tcctcctcga caaccaccgg
     8581 acgcccctcg tcctcgacga gctgcaatac gagcccgagc ttctcccctc gctcaaacgg
     8641 cgcatcgacc gggacaggag cccggggcgc gacgtcctca ccgggtcgca gcagtggggc
     8701 gctctcgcca ccgtcgccga gagcctcgcg ggccgcgcag ccctcctcga cctcgacggc
     8761 ttctccctcc gggagtccct cgaggcgacc gccgagaagc actggctcga cgcggcgctc
     8821 accggccagg agctgaaccg cagccagctc gggcgcgaga tcggcgtttc gcctcggtcg
     8881 gccgagcggt ggctcggaac gctgagagcg acgttccagt ggttcgaggt tcccgccttc
     8941 tccgggaaca ccgggaagag ggtcgcgtcg aaggcgaagg gctacgtcgc cgacaccggc
     9001 ttcgccggtc acgcgctcgc catctcgacg ccgaacgccg tcgcgagcca ccccgcctgg
     9061 ggcgccctct tcgagaccgc cgtctacgcg gagatccgcg aggctgtgtc cctgatttcg
     9121 cctcgccccg tcgtccacca ctggcgcacg ggcgcgggcg ccgaggttga tctcctcttc
     9181 gagcgggacg ggacgttctt cccgatcgag gtcaaggccg cgagccgccc gtcccgccgc
     9241 gacaccaacg ggatcaccgc gttccggaag acctacccga agctgagagt ggcccgaggc
     9301 ctcgtcgtcg ctccgtgcga gacgatggcc ccgctttcgg agaacgactg ggcgatgccg
     9361 tgggacgccg tgctcgctcc ggatcgggac ggaggggagt cccctcgcgt ccgatcccgc
     9421 ccgctcatac ctccccgagc ccccgctcca cgtccgcgac gaggtcctcc gtctcctcga
     9481 tcccgcacga gaggcggacg agcccgtcgg tgatgccgcg cgcggcacgc tcttcggggg
     9541 cgaggctgcc gtgggacatc gaggcggggt ggccggcgag ggactcgacg ccgccaagcg
     9601 actcggcgag ggtgaagagg cggagggcgt cgaggaagcg cttcgcggcg gcgtaggagc
     9661 cgagttcgac ggagagcatg cctccgaagc ccgacatctg gcgcgaggcg agggcgtgct
     9721 gcgggtggtc ggggaggccc gggtagagga cgcggacgac caccgggtgg acggcgagga
     9781 aggcggcgac ggcgcggccg ttcgcgtcgt gggcgcgcat ccgtagggcg agcgtcttga
     9841 cgccgcgcgt gacgaggaag gagtcg
//
LOCUS       DAOTEZ010000130         9863 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4856_length_9863_cov_6.388662, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000130 DAOTEZ010000000
VERSION     DAOTEZ010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9863
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4856_length_9863_cov_6.388662"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1728)
                     /locus_tag="P5164_08535"
     CDS             complement(<1..1728)
                     /locus_tag="P5164_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE sensor domain-containing protein"
                     /protein_id="HRY43976.1"
                     /translation="MSAPRVGSIRSRLMLIGMATSLSSLLLAAAAFVLVDRYRDRRGL
                     VEDVSTIARLVADRSTAALAFDDPGLAAENLSALRIKPAVTAACIYAADGSVFAEYRS
                     PRAPEYRFPAAEHEPTYRFEAGHLALFEPVLLDGTRLGTVHVRASLRELAVLRREYLL
                     SAAAIVAFASLLAFALSTRLQHLVSGPLARLTQTAQRIATQKDYSVRAPAEGKDEIAS
                     LARTFNEMVGVVEAESAASARAQAALRASEARLRRIVDLVPHMIFVKDRDGRFLLANR
                     TVAEAYGTTVPELLGRPQAELGLDREQARGWHEKDLQVLDEGLELFVAEEPFRDASGA
                     LRYLQTTKVPLLLEDGVTRAVLGVGIDVTERKRADDELKRYRDHLEDLVKERTAELAV
                     AKEKAEESDRLKSAFLATMSHELRTPLNSIIGFSGVLLQGLAGPLNEEQKKQMGMVCG
                     SAGHLLALINDVLDISKIEAGQLTLATETFDLRASLANLVRSAAPLAEKKGLSLEHEV
                     GPGVGEMTSDRRRVEQIVLNLLSNAVKFTDRGSVRLVCTTEGERATIRVADTGIGIRA
                     EDLDKLFRPF"
     gene            complement(1725..2336)
                     /locus_tag="P5164_08540"
     CDS             complement(1725..2336)
                     /locus_tag="P5164_08540"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HRY43977.1"
                     /translation="MDLLMRGTGPAPERSPERRSAHRPARARWAALALALLAATASAT
                     AEDPGLETRVKAAYVYNFLTFVEREKPEPEPEGAPVRVCVLGDEALLAQLKGLSGRPV
                     RGRPLVVLAGPEAGPLPSCHLLVIGRSEAARFPEILRTVSGTSVLTVSDIPRFSRRGG
                     MIGFLTEGGRVRIEVNPDAAAAAGLRMSAKLLEVARVVAEEVR"
     gene            complement(2324..3727)
                     /locus_tag="P5164_08545"
     CDS             complement(2324..3727)
                     /locus_tag="P5164_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRY43978.1"
                     /translation="MELARRRPRHIRRRDAEADRQGRPARGSGRRELTDAADNYENDL
                     SFDGLVRWKGLSVGFLVQDKEASRASVQKTLGEPLRDDGVGWHIRFLNAWARYSLQRP
                     GPWSAHSTLYVRSSTVEDDTVPVIEKATATSAGSQQRWYRPGELVGADTQATWAPAKR
                     FAVSAGLVLERERLSRSYSVTTSGSELEPALRPPEPERITNDVVSLYAEAEVGLTDEL
                     RLFAGLRHDDATSYGKVDTPRAGLVLNRGRLTAKLLYMEAFRAPKPWDLTDGLGNPGL
                     RPEEMRMVEASATYSFSPHLQADVSVYRSRLDDRLTRSGEGTQWRWVNAGDLDTRGVE
                     VSVELRKGRTTAWANASFCASEDETGRQVAEIARAGANAGVRYAVARRATLGLRATYV
                     GSRRNPKVIPATGDERIEDALVLRASASLALPHGLEARLFVDNLGGTTWFHPSNLPPS
                     RYRQPGRTFRVQAGWTF"
     gene            complement(3628..4344)
                     /locus_tag="P5164_08550"
     CDS             complement(3628..4344)
                     /locus_tag="P5164_08550"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRY43979.1"
                     /translation="MSGLCEIGRHGGTRPSALRCAPLALALLGAPLATAQEKSLDELL
                     QLSLEDLAAIEVVSAAKVPATAARVPATVRVITAETIAENGYLTLEDALADLPGFQFR
                     DILGFNSYAFLRGVPSQNNAILLLVDGLQVNELNSGGFYGGGQLNLANVERIEVVYGP
                     ASALYGTNALSGIVNVIRKDSGKGPGGKVNVSLGSLDTSLLDARYAWSSPGDDLAISV
                     AGMRKRTDKADLRGDRGDGN"
     gene            complement(4477..5448)
                     /gene="corA"
                     /locus_tag="P5164_08555"
     CDS             complement(4477..5448)
                     /gene="corA"
                     /locus_tag="P5164_08555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HRY43980.1"
                     /translation="MLSIHLLEDGKGLQLLEGATLGDAWPGLAGRSIWVDVEDPTEEE
                     ARALAEVCGFHPLTVEDSLTPVQPPKVEEFGSYLFLLFRGLRPTEEGLELESYKLAAW
                     LGPGFLVTAHRRPVAACREVSRLVLDKDLPMESQMDRILHALVDRVMDEILPILDRCE
                     ERLEELEEGVFSGAPQREVLQELLGLKRQLLTIRRLVVPQREMLGRLARRDLPYIDER
                     TSHYFRDVYDHVARIEETTIVLTDLASGALEAQLSVSSNRLNEIVKVLTIFSAIWMPL
                     TFLAGVYGMNFEFLPELKIRWFYPALWGGWIAITLGMLGVFRWRRWI"
     gene            5580..7598
                     /locus_tag="P5164_08560"
     CDS             5580..7598
                     /locus_tag="P5164_08560"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HRY43981.1"
                     /translation="MKKGTRILLTGGGTAGHVNPALAIGAALAEEGSETLFVGVRGRA
                     EEQVVPKEGIPIRFVRASGFPGARPSPAFARFLLDLAVGTIQAAGILLRFRPDVLVGT
                     GGFASAPVIFAATLLKKLRLLRSRVFVHEQNAVPGKLNRLVGRYAEKVFVTFPETLSL
                     FPGNGAVTGYPLRKRIGHVSKEEARSRLDFALPEGRTVVFVFGGSQGSRSLNRGLVDA
                     LGELLPHADRLFLVHGTGLFKGGGYDAGADTAARLAARYTPEERERIDSFYVSRSYFH
                     DIASVYAVSDLVVIRGGSGSLNEVARMGLPALVVPKANLPGDHQVANARALERAGGAE
                     VLYEETVLRDGHLAEELDGSLLARRVLDLVDDPDRLAEMSRMGRDVLGADAAAEIAHH
                     VRHGHGTGTLEPTLVPAPHVVSNAALLSRLEKAYAAEGRRYRVEAHAEARDLPWFRSR
                     AASLLANESWEVRNLGVKLLGLLGATEKVPLLLALLADRTPAPLLQRLLGGDFAQVGF
                     VRRNAFTALGRLGVVDAGVEAALLAGLSDPYWEVRAEAARTAARFAGLLSRRADVVAA
                     LVGGLSDRNLEAAAAAAEALGAIGGEADALPALLRLSDYRFWKVRAAALRGVLALVER
                     GEARDRTKLEAALPGFLLTSTDFRPQFEMKLSYRRVLEALSRGKESPR"
     gene            7595..8698
                     /gene="mraY"
                     /locus_tag="P5164_08565"
     CDS             7595..8698
                     /gene="mraY"
                     /locus_tag="P5164_08565"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRY43982.1"
                     /translation="MIFWIGKLLAPFPQLSFLRLAEYLSARSIGAALTAVLLTLWVMP
                     RVIWWLQRKAFVDQLRETGIPSAFDKAGTPVMGGVVLVGVVAFSSLLWCNLTSGYAFL
                     VLLGMVWFGAIGFVDDRAKMRARSGDKGMSEPRKLLLQGAFAAFFVAVLASPWSPVPP
                     AEAARLHVPFLKAAVLDAPWVYFPFVFLFVLLVGNSVNITDGMDGLAIVPSVFAIAVL
                     GVFGYLLGNAIWSRYLVYPFLPGAGELAVFACAFAGAGVGFLWFNAYPAQIFMGDTGS
                     LSIGGSLAVASVLLKQELLFVILGGLFVAEAFTSQVQDKIGIKLLGRRLFARAPLHHA
                     MAYNGLAETKVVVRLWIVSGILALAALSTLKLR"
     gene            8695..>9863
                     /gene="murJ"
                     /locus_tag="P5164_08570"
     CDS             8695..>9863
                     /gene="murJ"
                     /locus_tag="P5164_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRY43983.1"
                     /translation="MSTARRSSSANAFLVGAGILLSRLAGFVRERVFAHYLGSSMAAD
                     ALRAALRAPNVLQNLFGEGSLSASLIPVYSRLLGEGREEEARRTARTAGTLLALLAVL
                     LSALGVLAAPLLVDLFTPGFSGERRELTVRLVRILFPGTGLLVLSAFCLGILNSHRRF
                     FLPYAAPVLWNGAIIAALVLAGTKAGAEIAVQAAWGAVAGSLLQLLVQLPQVARLVGT
                     LRPALDIGSPHVRTIFRNLLPSVGSRGVTQVSSWVDQVIASFLPGGAVAMLGYAQTLY
                     ILPVSLFGMSVSSAELPELSSGAGAETLRERLDAALRRVAFLVVPSAVAFAFLGDRVV
                     ALVFQTGAFGAEDVRIVWAVLAGSSVGLLASTLGRLYASTFWALHDTRTPLRFAML"
BASE COUNT         1274 a         3553 c         3541 g         1495 t
ORIGIN      
        1 gaacggccgg aagagcttgt cgaggtcctc tgcgcggatc ccgatcccgg tgtcggcgac
       61 gcggatcgtc gcgcgctcac cctcggtagt gcagacgagc cgcacagagc cgcggtccgt
      121 gaacttcaca gcgttcgaga ggaggttgag gacgatctgc tccacgcggc gccggtcgct
      181 cgtcatctcc ccgaccccgg ggccgacctc gtgctcgagc gagagcccct tcttctccgc
      241 caggggcgcc gcggagcgga ccaggttcgc gagcgaggcc cggaggtcga aggtctccgt
      301 ggcgagcgtg agctggcccg cctcgatctt ggagatgtcg aggacgtcgt tgatcagggc
      361 gagcagatgc ccggcgctgc cgcagaccat ccccatctgc ttcttctgct cctcgttcag
      421 cgggccggcg agcccctgca gcaggacgcc cgagaagccg atgatcgagt tcagcggcgt
      481 gcggagctcg tgggacatgg tggcgaggaa cgcagacttc aggcggtcgg actcctccgc
      541 cttctccttg gcgacggcga gctcggcggt ccgctccttc acgaggtctt cgaggtggtc
      601 gcggtacctc ttcagctcgt cgtcggccct cttgcgctcg gtgacgtcga tgccgacccc
      661 gaggaccgcc cgggtgacac cgtcctcgag gaggagcggg accttcgtcg tctggaggta
      721 gcggagcgcg cccgaggcgt cccggaacgg ctcctcggcg acgaagagct cgagcccctc
      781 gtcgaggacc tgcaggtcct tctcgtgcca gccgcgagcc tgctcccggt cgagcccgag
      841 ctccgcctgt ggccggccga ggagctccgg gaccgtcgtg ccgtacgcct cggcgacggt
      901 ccgattggcg aggaggaacc gcccgtcgcg gtccttgacg aagatcatgt gggggaccag
      961 gtcgacgatc cgccggagcc gggcctcgga cgcgcggagc gcggcctggg cccgggcgct
     1021 ggcggcgctc tccgcctcga cgacgccgac catctcgttg aacgtcctcg cgagcgaggc
     1081 gatctcgtct ttcccctccg cgggcgcgcg gacggagtag tccttctggg tcgcgatgcg
     1141 ctgcgccgtc tgggtgagcc gggcgagcgg cccggagacg aggtgctgca gccgcgtcga
     1201 gagggcgaag gcgaggagcg aggcgaacgc gacgatggcc gccgcggaga ggaggtactc
     1261 ccgccggagg accgcgagct ctcgcaggct cgcgcgcacg tggaccgtgc cgagccgcgt
     1321 cccgtcgagg aggaccggct cgaagagggc gagatgcccg gcctcgaacc ggtacgtcgg
     1381 ctcgtgctcc gccgcgggga agcggtactc cggcgcgcgg ggggagcggt actcggcgaa
     1441 gacggagccg tcggcggcgt agatgcaggc cgccgtgacg gcgggcttga tgcgcagcgc
     1501 ggacaggttc tccgcggcca gtcccgggtc gtcgaaggcg agggcggccg tgctccggtc
     1561 cgcgacgaga cgggcgatcg tggagacgtc ctcgacgagg ccccggcggt cgcggtaccg
     1621 gtcgacgagg acgaaggcgg cggcggcgag gaggagagag ctgaggctcg tcgccatgcc
     1681 gatgagcatg agccgggagc ggatggagcc gacgcgcgga gcgctcaccg gacctcctcc
     1741 gccacgacgc gcgcgacctc gagcagcttc gcgctcatcc ggagaccggc cgcggcggcc
     1801 gcgtcgggat tgacctcgat ccgcacgcgg cccccctccg tcaggaagcc gatcatgccg
     1861 ccgcggcgcg agaagcgagg gatgtcgctc acggtcagga cgctcgttcc cgagacggtc
     1921 cggaggatct ccgggaatcg ggccgcttcg gagcggccga tgacgaggag atggcaggag
     1981 ggaagaggac cggcctccgg tccggcgagg accacgaggg gccggccgcg gaccgggcgg
     2041 cccgagaggc ccttcagctg cgcgaggagc gcctcgtccc cgaggacgca gacgcgcacc
     2101 ggggcgccct ccggctccgg ctcgggcttc tcccgctcga cgaacgtcag gaagttgtag
     2161 acgtacgccg ccttgacgcg cgtctcgagc cccgggtcct ccgccgtcgc ggaggccgtc
     2221 gcggcgagga gggcgagcgc gagcgccgcc caccgcgccc gcgcgggccg atgcgcggac
     2281 cggcgctcgg gcgagcgttc cggcgcaggg ccggtgccgc gcatcagaag gtccatcccg
     2341 cctggacgcg gaacgtccgc cccggctgac ggtagcggct cggggggagg ttcgagggat
     2401 ggaaccaggt cgtcccgccg aggttgtcga cgaagagccg agcctcgagc ccgtggggga
     2461 gggccagcga ggccgacgcg cggaggacga gggcgtcctc gatcctctcg tcgccggtgg
     2521 ccgggatgac cttcgggttc ctgcgggagc cgacgtacgt cgcgcgaaga ccgagcgtcg
     2581 cgcgccgcgc cacggcgtac cggacgccgg cattcgcgcc ggcacgggcg atctccgcca
     2641 cctggcgtcc cgtctcgtcc tccgacgcgc agaaggaggc gttcgcccag gccgtcgtcc
     2701 gccccttccg gagctccacc gagacctcga cgccacgcgt gtcgaggtct ccggcgttca
     2761 cccagcgcca ctgggtcccc tcgcccgacc gggtcagcct gtcgtcgagc cggctgcgat
     2821 agaccgagac gtccgcctgc aggtggggag agaaggagta cgtggccgac gcctcgacca
     2881 tccgcatctc ctccgggcga aggccggggt tcccgaggcc gtccgtcagg tcccacggct
     2941 tcggcgcccg gaacgcctcc atgtagagga gcttggccgt caggcggccg cggttcagga
     3001 cgagcccggc ccggggggtg tccaccttcc cgtagctcgt cgcgtcgtcg tgacgaagcc
     3061 ccgcgaagag ccggagctcg tcggtgaggc cgacttccgc ctcggcgtag aggctgacga
     3121 cgtcgttcgt gatccgttcg ggctcgggag ggcgaagggc gggctcgagc tccgagccgc
     3181 tcgtcgtgac tgagtagctt ctcgagagcc gctcccgttc gaggacgagc ccggccgaga
     3241 ccgcaaagcg cttcgcggga gcccaggtcg cctgcgtatc cgcgccgacc agctctcccg
     3301 gccgatacca gcgctgctgg cttccggccg aggtggcggt ggccttctcg atcacgggca
     3361 ccgtgtcgtc ctcgaccgtg ctgctcctca cgtagagggt cgaatgagcc gaccaggggc
     3421 cgggccgctg gagcgagtag cgggcccagg cgttcaggaa gcggatgtgc cagccgacgc
     3481 cgtcgtcccg gagaggctcg ccgagcgtct tctggacgct cgcgcgggag gcctccttgt
     3541 cctggaccag gaagccgacg ctcaggccct tccagcggac gaggccgtcg aacgagaggt
     3601 cgttctcgta gttgtcggcc gcgtcggtca gttcccgtcg ccccgatccc cgcgcaggtc
     3661 ggccttgtcg gtccgcttcc gcatcccggc gacggatatg gcgaggtcgt ctccgggcga
     3721 gctccaggcg taccgggcgt cgaggaggga ggtgtcgagg ctcccgaggg agacgttcac
     3781 cttcccgccc ggcccctttc cggagtcctt ccggatcacg ttcacgatgc ccgagagggc
     3841 gttcgtgccg tagagcgccg aagccgggcc gtagaccacc tcgatccgct cgacgttcgc
     3901 gaggttcagc tggccgccgc cgtagaagcc gcccgagttc agctcgttga cctggaggcc
     3961 gtcgacgagg aggaggatcg cgttgttctg gctcggcacg ccccggagga acgcgtagga
     4021 gttgaagccg aggatgtccc gaaactggaa ccccggcagg tcggccaggg cgtcctccag
     4081 ggtgaggtag ccgttctccg cgatcgtctc ggccgtgatc acccggacgg tggccgggac
     4141 ccgggcggcg gtggcgggca ccttcgcggc cgagacgacc tcgatcgcgg cgaggtcctc
     4201 gaggctgagc tggagcagct cgtcgaggct cttctcctgc gccgtggcga ggggcgctcc
     4261 gaggagggcg agcgcgaggg gggcgcatcg gagggccgac ggcctcgtgc ctccgtggcg
     4321 tccgatttcg caaaggccgc tcacgaccgg gagaccccgt atggcacgag tcacctccgg
     4381 cgcccggcga aaattctacg ctcggccgac gggcccggac gcgccctttc gatccgggcg
     4441 ggcggggtcc gcggatcggg agccccgggg gtcgtcctag atccagcggc gccagcggaa
     4501 gacgccgagc atcccgaggg tgatcgcaat ccagccgccc cacagggcgg ggtagaacca
     4561 gcggatcttc agctccggca ggaactcgaa gttcatgccg tagacgccgg cgaggaaggt
     4621 gagcggcatc cagatcgcgg agaagatcgt caggaccttc acgatctcgt tcagccggtt
     4681 cgaggagacc gagagctgcg cctcgagcgc gccgctcgcg aggtcggtca ggacgatcgt
     4741 cgtctcctcg atacgggcga cgtggtcgta gacgtcgcgg aagtagtgtg aggtccgctc
     4801 gtcgatgtac ggcaggtcgc ggcgggcgag gcggccgagc atctccctct gcgggacgac
     4861 gaggcggcgg atcgtcagca gctggcgctt caggcccaga agctcctgga gcacctccct
     4921 ctggggcgcc cccgagaaga ccccctcctc cagctcctcg aggcgctcct cgcagcggtc
     4981 gaggatcggg aggatctcgt ccatcacgcg gtcgacgagc gcgtggagga tccggtccat
     5041 ctggctctcc atcggcaggt ccttgtcgag gacgagacgc gagacctccc ggcaggccgc
     5101 gacggggcgc cggtgggccg tgacgaggaa gccggggccg agccaggcgg cgagcttgta
     5161 gctctcgagc tcgagcccct cctccgtggg ccggagaccc cggaagagga ggaagaggta
     5221 gctcccgaac tcctcgacct tgggcggctg gacgggggtg agcgagtcct cgacggtgag
     5281 cgggtggaag ccgcagacct cggcgagggc gcgggcctcc tcttccgtcg ggtcctcgac
     5341 gtcgacccag atcgagcggc ccgcgaggcc gggccacgcg tcgccgagcg tcgcgccttc
     5401 gaggagctgg agccccttac cgtcctcgag caggtggatg gagagcatcg ggcgacgagt
     5461 ctatccgcgg aggggcggac gaaggccgga aacgggggag aatgccgggc tcggcccgcc
     5521 gggagcaccg gacgttcgcg gcggcccgaa tgccccagaa tcgggcggag gcgcgaggct
     5581 tgaagaaggg aacgaggatc ctcctgacgg gaggcggcac cgccgggcac gtgaacccgg
     5641 cgctcgccat cggagcggcc ctggccgagg aggggagcga gacgcttttc gtcggcgtgc
     5701 gagggcgggc cgaggaacag gtcgtcccga aggaggggat cccgatccgc ttcgtgaggg
     5761 cctcgggctt ccccggggca cgtccctcgc cggcgttcgc gcgcttcctc ctcgacctcg
     5821 cggtcgggac gatccaggcc gccggcatcc tcctccgctt ccgccccgac gtcctcgtcg
     5881 ggacgggggg cttcgcctcg gcgcccgtca tcttcgcggc gacgctcctg aagaagctcc
     5941 gcctcctccg gtcccgcgtc ttcgtccacg agcagaacgc cgtccccggg aagctgaacc
     6001 ggctcgtcgg ccgctacgcc gagaaggtct tcgtcacgtt ccccgagacg ctctccctct
     6061 tcccggggaa cggggcggtc accggctacc cgctccggaa gcggatcggc cacgtctcga
     6121 aagaggaggc gcgctcccgc ctcgacttcg cgctcccgga ggggcggacc gtcgtcttcg
     6181 tcttcggcgg gtcgcagggc tcgcgctcgc tcaaccgcgg cctcgtcgac gcgctcggcg
     6241 agctcctccc gcacgccgac cgcctcttcc tcgtccacgg gaccgggctc ttcaagggcg
     6301 gcggctacga cgccggcgcc gacacggccg cccgcctcgc ggcgcgctac acgcccgagg
     6361 agcgggaacg gatcgattcc ttctacgtgt cgcggagcta tttccacgac atcgcctcgg
     6421 tctacgccgt ttccgacctc gtcgtgatcc ggggcggctc ggggagcctc aacgaggtcg
     6481 cgcgcatggg cctcccggcg ctcgtcgtcc cgaaggcgaa cctgccgggc gaccaccagg
     6541 tcgcgaacgc ccgcgccctc gagcgggcgg gcggcgccga ggtcctctac gaggagacgg
     6601 tcctccgcga cgggcacctc gcggaggagc tcgacgggtc gctcctcgcg cgccgggtcc
     6661 tcgacctcgt cgacgacccg gatcggctcg ccgagatgtc gcggatgggg cgcgacgtcc
     6721 tcggggccga cgccgccgcc gagatcgcgc accacgtccg tcacggacac ggaacgggca
     6781 cgctcgagcc gacgctcgtc ccggcgccgc acgtcgtctc gaacgccgcg ctcctctccc
     6841 ggctcgagaa ggcgtacgcc gcggaggggc gtcgctaccg cgtcgaggcg catgccgagg
     6901 cgcgcgacct cccctggttc cggagccgcg ccgcttcgct cctcgcgaac gagagctggg
     6961 aggtgcggaa cctcggcgtc aagctcctcg gcctcctcgg ggcgaccgag aaggtcccgc
     7021 tcctcctcgc cctcctcgcc gaccggactc cggctccgct cctccagcgt ctcctcgggg
     7081 gcgatttcgc gcaggtcggt ttcgtccggc ggaacgcctt caccgcgctc ggccgtctcg
     7141 gcgtcgtcga cgccggcgtc gaggccgcgc tcctggccgg cctctcggac ccgtactggg
     7201 aagtgagggc cgaggcggcg cggacggcgg cgcgcttcgc cggcctcctc tcgcggaggg
     7261 ccgacgtcgt ggccgcgctc gtgggcggcc tctccgaccg caacctcgag gcggcggcgg
     7321 cggccgccga ggcgctcggg gcgatcggcg gcgaggccga cgcgctcccg gcgctcctcc
     7381 ggctctccga ctaccggttc tggaaggtgc gcgccgcggc ccttcggggg gtcctcgcgc
     7441 tcgtcgagcg gggcgaggcg cgcgatcgga cgaagctcga agcggcgctc ccggggttcc
     7501 tcctgacgtc gaccgacttc cgccctcagt tcgagatgaa gctctcgtac cgccgcgtcc
     7561 tcgaggcgct ctcgcgcggc aaggagtcgc cccgatgatc ttctggatcg gcaagctcct
     7621 cgcgccgttc ccccagctct ccttcctccg cctcgccgag tacctctcgg cgcgctccat
     7681 cggcgcggcg ctcacggccg tcctcctgac gctctgggtc atgccgcgcg tgatctggtg
     7741 gctccagcgg aaggcgttcg tcgaccagct ccgcgagacg ggcatcccgt cggccttcga
     7801 caaggccgga acgccggtca tggggggcgt cgtcctggtg ggcgtcgtcg cgttctcctc
     7861 gctcctctgg tgcaacctga cgagcggcta cgccttcctc gtcctcctcg gcatggtctg
     7921 gttcggggcg atcggcttcg tcgacgaccg ggcgaagatg cgggcgaggt cgggcgacaa
     7981 ggggatgtcg gagccgcgga agctcctcct gcagggggcg ttcgcggcgt tcttcgtcgc
     8041 cgtcctcgcg agcccctgga gcccggtccc gcccgctgag gcggcccgcc tccacgtccc
     8101 cttcctgaag gccgcggtcc tcgacgcccc gtgggtctac tttccgttcg tcttcctctt
     8161 cgtcctcctc gtgggcaact ccgtgaacat caccgacggg atggacggtc tcgcgatcgt
     8221 cccgtcggtc ttcgcgatcg ccgtcctcgg cgtcttcggc tacctcctcg ggaacgcgat
     8281 ctggtcccgg tacctcgtct acccgttcct gccgggagcg ggcgagcttg ccgtcttcgc
     8341 ctgcgcgttc gcgggggcgg gcgtcggctt cctctggttc aacgcctacc cggcccagat
     8401 cttcatgggg gacaccgggt cgctctccat cggcggaagc ctcgccgtcg cgtcggtcct
     8461 cctgaagcag gagctcctct tcgtcatcct cggcggcctc ttcgtcgccg aggcgttcac
     8521 ctcgcaggtg caggacaaga tcggcatcaa gctcctcggg cggaggctct tcgcgcgagc
     8581 gccgctccac cacgcgatgg cctacaacgg gctcgccgag acgaaggtcg tcgtgaggct
     8641 ctggatcgtg tcggggatcc tcgccctcgc cgccctctcg acgctgaagc tgcggtgagc
     8701 acggcgcggc gctcctcgtc ggcgaacgcc ttcctcgtcg gggccggcat cctcctgagc
     8761 cgcctcgcgg gattcgttcg ggagcgcgtc ttcgcccact acctcggcag ctcgatggcg
     8821 gccgacgccc tccgggcggc gctgcgcgcc ccgaacgtcc tccagaacct cttcggcgaa
     8881 ggctcgctct cggcctcgct catcccggtc tactcccgtc tcctcggcga ggggagggag
     8941 gaggaggcgc ggaggacggc gcggaccgcc gggacgctcc tcgcgctcct cgccgtcctc
     9001 ctctccgcgc tcggcgtcct cgcggcgccc ctcctcgtcg acctcttcac gcccggcttc
     9061 tcgggcgaga ggcgcgagct gacggtacgg ctcgtccgga tcctcttccc cggaaccggc
     9121 ctcctcgtcc tctcggcctt ctgcctcgga atcctcaaca gccaccgccg cttcttcctg
     9181 ccgtacgcgg cgcccgtcct ctggaacggc gcgatcatcg ccgcgctcgt cctcgcgggg
     9241 acgaaggccg gagccgagat cgcggtccag gccgcctggg gcgccgtcgc cgggagcctc
     9301 ctccagctcc tcgtccagct cccgcaggtg gcccgcctcg tcggcacgct ccgtcctgcg
     9361 ctcgacatcg ggtcgcccca cgtgaggacg atcttccgca acctcctccc gagcgtgggg
     9421 agccggggcg tgacacaggt ctcgagctgg gtcgaccagg tcatcgcgag cttcctgccg
     9481 ggcggcgccg tcgcgatgct cggctacgcg cagacgctct acatcctccc ggtctcgctc
     9541 ttcggcatgt cggtctcgag cgccgagctc cccgagctct cctcgggggc gggcgccgag
     9601 acgctccgcg agcggctcga cgccgcgctc cggcgggtcg ccttcctcgt cgtcccgtcg
     9661 gccgtcgcgt tcgcgttcct cggcgaccgc gtcgtcgccc tcgtgttcca gaccggcgcc
     9721 ttcggagccg aggacgtccg catcgtctgg gcggtcctgg ccggatcctc ggtcgggctc
     9781 ctcgcctcga cgctcggaag gctctacgcc tcgacgttct gggcgctcca cgacacgcgg
     9841 accccccttc gcttcgcgat gct
//
LOCUS       DAOTEZ010000131         9859 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4858_length_9859_cov_5.264076, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000131 DAOTEZ010000000
VERSION     DAOTEZ010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9859
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4858_length_9859_cov_5.264076"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(295..1617)
                     /locus_tag="P5164_08575"
     CDS             complement(295..1617)
                     /locus_tag="P5164_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43984.1"
                     /translation="MTPRTLRLALAALAALSFAPAAHAQSGGWGRITLFLQGGQTRPE
                     DGGESTSSNEAIASVTVRSAEREESGLEYALDGRGASYGMEGEERRSRLSLWEAWVGG
                     RFAHGAVAVRVGQQWITDLGGLGSVGGALVEVRGRAVKGEGKLRPRLGVFAGVEPKGW
                     ETGWESDVRKGGAYAALDGEGGRRHVLGFVTIRNSGLTERSVLSTTNFVPFGRTLFLY
                     VAGEYDVASPGGRGEKGLSYAFVNARWTLSPRVELQGLYHRGRSYDARTITQDQLDGR
                     PVDPRLLAGFLYESGGGRLTVEVARGLRVFAGYSQDRGNRDSGSTGRITAGLHAADVF
                     RTGIDVTVSDNRIDRPDGAYDSWYASVGRSFGGRVYVSLDYATALSVLRFTDADGLVV
                     ETRPRTDRFSLNGNVNLGRSLSLLLAAERTSGDLSTELRGSAGFAVRF"
     gene            complement(1629..>1920)
                     /locus_tag="P5164_08580"
     CDS             complement(1629..>1920)
                     /locus_tag="P5164_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HRY43985.1"
                     /translation="PNHAAAGFPTTCETCHRASDSSWNQGTFNHTWFPITTGKHAGNP
                     CSACHTSPANYRVFTCLTCHGRAETDRKHQGRAGYRYDSAACYSCHPQGRGD"
     assembly_gap    1921..2020
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2021..5996)
                     /locus_tag="P5164_08585"
     CDS             complement(<2021..5996)
                     /locus_tag="P5164_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43986.1"
                     /translation="MTSLAPVLAAALLLAAPAAAQLGELVSPGPLSSAHAKLEGLRSC
                     EKCHERGNRVSAARCLACHADVASRIAAKKGVHRDVADECAACHAEHAGRDAELRPFE
                     TKGFDHAGETGFPLDGKHAPLAGRCASCHRTRSFLKLSPSCATCHADPHKGALGAACA
                     TCHSTAVAFKETRRTFDHAKTRFPLSGAHVRVACAKCHANAVFRGLKFGSCADCHQDP
                     HRKAFGPDCSACHETKTFRTTKVDHAKTTFPLRGRHAEIACAACHREPATKVKLKAGS
                     CATCHQDPHRGEFKQDCASCHTEMGFKGTPFDHAGKTRFPLTGRHAAAKCTACHVPPG
                     GAAPATNGRTPKVVVFRGLKAECAACHADPHKGALGTACATCHGTESFRPKGFRHPRL
                     PELFAGAHEGLACEKCHGGPKTGPNGRPVPVGERTYRGLSTACASCHRDPHLGQVSSS
                     CESCHSVSGAKFAPLRFDHAKAAFPLAGRHASLACAKCHRQESGTFPAGRGTAVRLKG
                     LPTTCVGCHRDPHLGQLGTSCATCHSAESFKVPSFRHPGRPEFWKGAHAKAACAACHK
                     KEEGAFPAGKGTAIRYRGFKASCASCHEDPHRGALGAGCESCHTPSSAWKTASRAFHK
                     TSWPLEGKHLLVPCASCHVKGQTKGTPRDCADCHWVRRQDDRFKTALGLDCQRCHRPS
                     SWTAVTWNHAAATGFALAGAHASIDCEKCHTGGIFRKGESGCVGCHRADYDRAKEPNH
                     AAAGFPTTCETCHKVSDGSWHQARFDHRGVFPLSGRHATAACASCHRNGVYRGTARDC
                     AGCHLPLYQKATNPSHILAGFPTTCESCHKPTDASWMGVAFNHARWPLAGSHAAQACS
                     ACHRSGVFQGLPSSCVSCHQADYQRAASPNHAAAGFPTTCETCHRASDASWRLGRFDH
                     ASTFVLVGRHATATCASCHSSGVYRGTARDCAGCHLAAFQKATNPNHLMAGFSTTCQT
                     CHRATDASWTAGTFDHSKWPLLGRHAGNACTACHRTGVFQGLATACASCHQADYQRAA
                     NPNHAAAGFPTTCETCHRLSDASWRQGTFTHSKWPLQGRHASPACATCHRNGVFQGLP
                     SDCVSCHLPNYQATTNPNHAAAGFPTTCEACHRATDSSWNQGTFNHGTVFALQGRHAT
                     AACASCHRNGVYRGTPRDCASCHLSRYQATTNPNHAQAGFPTTCETCHRATDTAWSQG
                     TFNHGTVFALQGRHATAACASCHRNGVYRGTPRDCASCHLPRYQATTNPNHAAAGFPT
                     TCETCHRATDTAWSQGTFNHGTVFALQGRHATAACASCHRNGVYRGTPRDCASCHLSR
                     YQATT"
     gene            complement(5993..6706)
                     /locus_tag="P5164_08590"
     CDS             complement(5993..6706)
                     /locus_tag="P5164_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43987.1"
                     /translation="MGRSFGIAGSVLMFGGTLAYVARKRLRVLSNVGSTKAWLEAHIF
                     CGILGPALVTLHTSFRFNGIVSVAYWSMLGVVASGFVGRYLYVRIPRTIRGVEMDRKA
                     IEERAAELRRQLAEMTVPVRLLLRIEEMERELVPPAGDGPGLASLLLGGIAIRRKTRA
                     IERTIRSAGLTRRLVREALYAEAERIALIRRAATLDRTRRLFALWHVFHRPFVWVLAL
                     VFVLHVGVAVYFGYAAGGR"
     gene            complement(7072..8388)
                     /locus_tag="P5164_08595"
     CDS             complement(7072..8388)
                     /locus_tag="P5164_08595"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRY43988.1"
                     /translation="MASPAPVAAVAFITTLHLAAVLLLAHRRAGEGRRALLLPSIALP
                     VFAWLLPAPAWLIAGLAAQAAWVVACEKLLGGGRDEAAPAGPAAPRSRAASPGAAAPG
                     EPGPRAGFVPVKVLSVVDETPEIRTFRVERPAGFAFRAGQFLIVRVPAGGREEVRCYS
                     ISSAPEASGYLEISVRRQGRASGALHETVRAGGVLAVRGPGGSFVYPPEPEAPIVLHA
                     GGVGITPMISMLRHAASSEPGRPVTLLYTVRTEADVAFRDELSLLARRAPGVRVHVAL
                     SRGEPRPGYLTGRMDAGRIAQFVPDAAKAIHLICGPAPMIASLRESLAGLGVPPERVR
                     FEAFEAAVASAADAAAEGAAHELRFARSGLATTVTEATSLLEAAEGAGVEIESMCRAG
                     NCGTCRTRLLSGSVRDPGSALGAEERKNGWVLACVSRAAGDCVLDA"
     gene            complement(8458..>9859)
                     /locus_tag="P5164_08600"
     CDS             complement(8458..>9859)
                     /locus_tag="P5164_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HRY43989.1"
                     /translation="ALSGVGVGGGSLVYANTLPVPKKPFFEAPSWARLARWEEELAPH
                     YRTALAMLGAARTPFLTKPDEVIRDIGRQIGRGDGFESTNVAVFFGREGQGDKPGDTV
                     PDPFFGGKGPERTVCTRCGGCMVGCRHGAKNTLDRNYLFLAEGLGAVVKADTEVTWVK
                     PLPGGGYELSARVGGAPVPALRKEERITARNVVFSGGVLGTVKLLLELKESPEGLPAL
                     SPRVGDFVRTNSEVLVGVVTTKDEDLSQGVAIGSILHTDEHSHLEPVRYSKGSGFLRL
                     LALPFAPGDTLFQRFAHAFSTILASPWKATKSFWVPDWAKRTMILLYMRTVDGHLRMT
                     LGRSWRTGFRRGLSSVAGGGAGAKAWHPEANDLTKRACEALDGFPQSILTETLLGIPT
                     TAHILGGCPMGDSPENGVIDHRHRVFGYEGLWVVDGSAISANPGVNPSLTITALAERA
                     MTFVPPKGDAGAGATA"
BASE COUNT         1303 a         3589 c         3537 g         1330 t
ORIGIN      
        1 gacgacgggc acgccgagcc cgcgcagccg cgcgatcgcc gcccgcgcct cgtctccgag
       61 gacgcccccg tgctcgagga gggtcccgtc gaggtccgtg agcacgcatc cgatccgcac
      121 gacgtctcct ccgaacgggt ttcgccacga agcgccccgg cgcgcgggcc ctcgcgcgcg
      181 cctctctttc cgcctattcg tcgggcccgg ggctgccgaa gcgacaggcc tgccgggccg
      241 gagagtgaag acagctcgcg gagcgaactt agcacacggg ggagggccgg gccgtcagaa
      301 cctcacggcg aagcccgcgc tcccgcgcag ctcggtcgag aggtcgccgc tcgtccgctc
      361 cgcggccagg aggagcgaga ggctgcgccc caggttcacg ttcccgttca gcgagaagcg
      421 gtcggtccgc ggacgcgtct cgacgacgag cccgtccgcg tccgtgaacc ggagcaccga
      481 gagcgccgtc gcgtagtcga gcgagacgta gacgcgcccg ccgaagctcc gcccgaccga
      541 cgcgtaccag gagtcgtagg ccccgtccgg ccggtcgatg cggttgtccg acaccgtcac
      601 gtcgattccc gttcggaaga cgtccgccgc gtggaggcct gccgtgatcc ggcccgtcga
      661 gccgctgtcg cggttccccc ggtcctggga gtagccggcg aagacgcgca ggccgcgcgc
      721 gacctccacg gtcagcctcc ccccgcccga ctcgtacagg aatccggcga ggaggcgcgg
      781 gtcgaccggc cgcccgtcga gctggtcctg cgtgatcgtc ctcgcgtcgt acgagcggcc
      841 ccggtggtag aggccctgca gctcgacgcg cggggagagc gtccagcgtg cattgacgaa
      901 cgcgtagctc agccccttct cgccccggcc cccggggctg gccacgtcgt actcgccggc
      961 gacgtagagg aagagcgtcc ggccgaaggg gacgaagttc gtcgtcgaga ggacggacct
     1021 ctccgtcagg cccgagttgc ggatcgtcac gaagccgagg acgtggcgtc gccctccctc
     1081 gccgtcgagc gccgcgtagg cgccgccctt cctcacgtcg ctctcccagc ccgtctccca
     1141 ccccttcggc tcgactcccg cgaagacgcc gagccgcggc cgcagcttcc cttccccctt
     1201 cacggctcgc ccgcggacct cgacgagggc cccgccgacg gagccgaggc cgccgaggtc
     1261 ggtgatccac tgctgcccga cgcggaccgc gaccgcaccg tgcgcgaacc gcccgccgac
     1321 ccatgcctcc cagagggaga ggcggctccg cctctcttct ccctccatcc cgtacgacgc
     1381 gccgcgcccg tcgagggcgt actcgaggcc gctctcctcc cgttcggcgg accggacggt
     1441 cacggacgcg atcgcctcgt tcgacgaggt ggactctccc ccgtcctcgg gccgggtctg
     1501 ccctccctgc aagaagagcg tgatgcgccc ccagccgccg gactgggcgt gcgccgcggg
     1561 agcgaaggag agcgcggcga gagccgcgag ggcgaggcgg agagtcctcg gcgtcatcgc
     1621 gctcctcctc agtccccgcg cccctgcggg tggcacgagt agcaggcggc cgagtcgtag
     1681 cggtagccgg cgcgtccctg gtgcttcctg tccgtctccg cccggccgtg acaggtgaga
     1741 caggtgaaga cgcggtagtt cgccggcgag gtgtggcagg ccgagcaggg gttgccggcg
     1801 tgcttccccg tcgtgatcgg gaaccacgtg tgattgaacg tcccctggtt ccacgagctg
     1861 tccgacgcgc ggtggcacgt ctcgcacgtc gtcgggaagc ccgccgccgc gtggttcggg
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcgtcgtcgc ctggtacctc
     2041 gagagatggc acgaggcgca gtcccgcggc gtccctctgt agacgccgtt cctgtggcac
     2101 gaggcgcacg ccgcagtcgc gtggcgcccc tgaagagcga agaccgtccc gtggttgaac
     2161 gtcccctggc tccacgccgt gtcggtcgcg cggtggcacg tctcgcacgt cgtcgggaag
     2221 cccgccgccg cgtggttcgg gttcgtcgtc gcctggtacc tcgggaggtg gcaggaggcg
     2281 cagtcccgcg gcgtcccccg gtagacgccg ttccggtgac acgaggcgca cgccgcggtc
     2341 gcgtggcgcc cctgaagagc gaaaaccgtc ccgtggttga acgtcccctg gctccacgcc
     2401 gtgtcggtcg cccggtggca cgtctcgcac gtcgtcggga accccgcctg cgcgtggttc
     2461 gggttcgtcg tcgcctggta cctcgagaga tggcacgagg cgcagtcccg cggcgtcccc
     2521 ctgtagacgc cgttccggtg acacgaggcg cacgccgccg tcgcgtgccg cccctgcagc
     2581 gcgaagaccg tcccgtggtt gaacgtcccc tggttccacg acgagtccgt cgcccggtgg
     2641 cacgcctcgc acgtcgtcgg gaagcccgcc gccgcgtggt tcgggttcgt cgtcgcctgg
     2701 tagttgggca ggtggcacga cacgcagtcg ctcgggaggc cctggaagac cccgttcctg
     2761 tggcacgtcg cgcacgccgg gctcgcgtgg cgtccctgga ggggccactt cgagtgggtg
     2821 aaggtcccct gccgccacga cgcgtcggag agccggtggc aggtctcgca cgtcgtcggg
     2881 aagcccgccg ccgcgtggtt cgggttcgcc gccctctggt agtcggcctg gtgacacgac
     2941 gcgcacgcgg tcgcgagccc ctggaagacg ccggttcggt ggcacgccgt gcaggcgttt
     3001 cccgcgtggc ggcccaggag cggccacttc gagtggtcga aggtccccgc cgtccacgag
     3061 gcgtcggtcg ccctgtggca ggtctggcac gtcgtcgaga agcccgccat gaggtggttc
     3121 gggttcgtcg ccttctggaa ggccgcgagg tggcagccgg cgcagtcgcg ggccgtcccg
     3181 cggtagacgc cgctcgaatg gcaggaggcg cacgtcgccg tcgcgtgccg cccgacgagg
     3241 acgaaggtcg acgcgtggtc gaacctcccc agcctccacg acgcgtcgga ggcccggtgg
     3301 cacgtctcgc aggtcgtcgg gaagcccgcc gcggcgtggt tggggctcgc cgccctctgg
     3361 tagtccgcct ggtggcacga gacgcagctc gacgggagcc cctggaagac gccgctccgg
     3421 tggcacgccg agcaggcctg cgcggcgtgg ctcccggcga ggggccacct cgcgtggttg
     3481 aacgccacgc ccatccagga cgcgtcggtc ggcttgtggc agctctcgca cgtcgtcggg
     3541 aaccccgcca ggatgtggct cgggttcgtc gccttctggt agagcgggag gtggcagcca
     3601 gcgcagtcgc gggccgtccc ccggtagacg ccgttcctgt ggcacgaggc gcacgccgcc
     3661 gtcgcgtgcc tgccggagag cgggaacacc ccgcggtggt cgaaccgggc ctggtgccac
     3721 gagccgtccg agaccttgtg gcaggtctcg cacgtcgtcg ggaagcccgc cgccgcgtgg
     3781 ttcggctcct tcgcccggtc gtagtccgcc ctgtggcagc cgacgcagcc cgactccccc
     3841 ttccggaaga tcccgcccgt gtggcacttc tcgcagtcga tcgaggcgtg agcccccgcg
     3901 agcgcgaacc cggtcgccgc ggcatggttc caggtgacgg ccgtccagga agacgggcgg
     3961 tggcagcgct ggcagtcgag gccgagggcg gtcttgaagc ggtcgtcctg tctcctcacc
     4021 cagtggcagt cggcgcagtc gcgcggcgtc cccttcgtct gccccttcac gtggcaggac
     4081 gcgcacggga cgaggaggtg cttcccctcg agcggccagg aggtcttgtg gaacgcgcgc
     4141 gaggcggtct tccacgcgga agagggcgtg tggcagctct cgcagcccgc gccgagggcc
     4201 ccgcgatgcg ggtcctcgtg acaggacgcg cacgacgcct tgaagcccct gtagcggatg
     4261 gccgtcccct tccccgccgg gaacgccccc tcctccttct tgtggcacgc cgcgcacgcc
     4321 gccttcgcgt gcgctccctt ccagaactcc ggccggccgg ggtgccggaa cgaagggacc
     4381 ttgaagctct cggccgaatg gcaggtcgcg cacgaggtcc cgagctgacc gaggtgcggg
     4441 tccctgtggc agcccacgca ggtcgtcggg agccccttca gccggaccgc ggtccctctc
     4501 cccgccggga acgtgccgct ctcctgcctg tggcacttcg cgcaggcgag ggacgcgtgc
     4561 cggccggcga gcgggaacgc cgccttcgcg tggtcgaacc gcagcggggc gaacttcgcg
     4621 cccgacacgg agtggcagct ctcgcaggag gaggagacct ggccgaggtg cgggtctctg
     4681 tggcagctcg cgcaggccgt cgagaggccc cggtacgtcc tctcgccgac cgggaccgga
     4741 cgcccgttcg gtccggtctt cgggccgccg tgacacttct cgcaggcgag gccctcgtgg
     4801 gcgccggcga agagctcggg gaggcgcggg tgccggaagc ccttcggccg gaagctctcc
     4861 gtgccgtggc acgtggcgca cgccgtcccg agcgccccct tgtgagggtc cgcgtggcag
     4921 gccgcgcact ccgccttcag cccccggaag acgacgacct tcggcgtccg gccgttcgtc
     4981 gccggggccg cgccgcccgg cgggacgtgg cacgccgtgc acttcgccgc cgcgtgacgt
     5041 ccggtgagcg gaaatcgggt cttacccgcg tggtcgaacg gcgtgccctt gaagcccatt
     5101 tccgtgtggc agctcgcgca gtcctgcttg aactcgcccc ggtgggggtc ctggtggcag
     5161 gtcgcgcagc tccccgcctt cagcttcacc ttcgtggccg gctcccggtg acaggccgcg
     5221 cacgcgatct cggcatgccg gccccggagc gggaaggtcg tcttcgcgtg gtcgaccttc
     5281 gtcgtccgga aggtcttcgt ctcgtggcag gccgagcagt ccgggccgaa ggccttccgg
     5341 tgcgggtcct ggtggcagtc ggcgcagctt ccgaacttca gcccgcggaa gacggcgttc
     5401 gcgtggcact tcgcgcaggc cacacggacg tgcgctccgc tcagcgggaa cctcgtcttc
     5461 gcgtggtcga acgtccggcg ggtctccttg aaggcgacgg ccgtcgagtg gcaggtcgcg
     5521 caggcggcgc cgagggctcc cttgtggggg tccgcgtggc aggtggcgca cgacggcgag
     5581 agcttcagga aggagcgcgt cctgtggcac gacgcgcacc tcccggcgag cggggcgtgc
     5641 ttcccgtcga gggggaagcc ggtctctccg gcgtggtcga aacccttcgt ctcgaacggg
     5701 cgcagctccg cgtcgcggcc ggcgtgctcg gcgtggcagg ccgcgcactc gtccgccacg
     5761 tcccggtgga cccccttctt cgccgcgatc cgggaagcga cgtccgcgtg gcaggcgagg
     5821 cagcgcgccg ccgagacgcg gttcccccgc tcgtggcact tctcgcagct cctcagcccc
     5881 tcgagcttcg cgtgggcgga agagagcggc ccgggcgaca cgagctcgcc cagctgcgcg
     5941 gccgccggag ccgccagaag gagcgccgcc gcgaggaccg gcgcgaggct cgtcatcgtc
     6001 cacccgccgc gtagccgaag tagacggcca cgcccacgtg gaggacgaag acgagcgcga
     6061 ggacccagac gaaaggcctg tggaagacgt gccagagggc gaacaggcgc cgggtccgat
     6121 cgagcgtggc cgcccggcgg atgagggcga tccgctcggc ctccgcgtag agggcctccc
     6181 tcacgagccg ccgggtcagt cccgccgagc ggatcgtccg ctcgatcgcc cgggtcttcc
     6241 tccggatcgc gatccccccg aggaggaggc tcgcgaggcc cgggccgtcg cctgccggcg
     6301 ggacgagctc ccgctccatc tcctcgatcc ggagaaggag ccgaaccggg accgtcatct
     6361 ccgcgagttg acggcggagc tcggccgccc gctcctcgat cgccttccgg tccatctcga
     6421 cgccgcggat cgtcctcggg atccggacgt agaggtagcg gccgacgaac cccgacgcga
     6481 cgacgccgag catcgaccag taggcgacgg agacgatccc gttgaagcgg aacgacgtgt
     6541 ggagggtgac gagggccggc ccgaggatgc cgcagaagat gtgggcctcg agccacgcct
     6601 tcgtgctccc gacgttcgag aggacgcgga gcctcttcct cgcgacgtag gcgagggtcc
     6661 cgccgaacat gaggacggag ccggcgattc cgaaggacct ccccacgggg cccgccggcc
     6721 ggagcgcccg gtgctccggc tcgtaggcac gtgtgcgaag cggggtcctg tagtaggaga
     6781 gaccaccgag cccgaagagc acgacgagaa cggccccgat cgcggcggat ccggccagag
     6841 acaggacacc gaacggcctc cgtgtcctcg tccggacgga accgtcgacg tcgaatacac
     6901 gtccaggctg ggccccctgc gggaccgcgc ggctctcttc catccccctc ctgccagcga
     6961 cggggggagg tcgcgcccga taccgacgga cctctcgctg ggtttcacgg gagtgtcgga
     7021 gatcgaggaa tcccacgcat ccgtcttccg aacggaagcc gaaccgcgcg tttacgcgtc
     7081 gaggacgcag tcccccgccg cgcgggagac gcaggcgagg acccacccgt tcttccgctc
     7141 ctccgcgccg agagcggagc cgggatcgcg gacgcttccc gagaggagac gcgtcctgca
     7201 ggtgccgcag ttgccggccc ggcacatcga ctcgatctcc accccggcgc cctcggcggc
     7261 ttcgaggagg ctcgtcgctt ccgtgaccgt cgtcgcgagg ccgctccgcg cgaatcggag
     7321 ctcgtgagcg gcgccctcgg ccgccgcgtc ggccgccgac gccacggcgg cctcgaaggc
     7381 ctcgaaccgg acccgctccg gcgggacgcc gaggccggcg agcgactcgc ggagcgacgc
     7441 gatcatcggg gccgggccgc agatcaggtg gatcgccttc gccgcatccg ggacgaactg
     7501 cgcgatccgc ccggcgtcca tccggcccgt caggtagccc ggcctcggct cgccccggct
     7561 gagcgcgacg tggacgcgga ctcccggcgc gcggcgtgcg aggagggaga gctcgtcacg
     7621 gaacgcgacg tccgcctcgg tccgcaccgt gtagaggagc gtcaccggcc gccccggctc
     7681 cgagctcgcc gcgtggcgga gcatggagat catcggcgtg atgccgacgc cgcccgcgtg
     7741 gaggacgatc ggcgcctccg gctccggcgg gtagacgaag gagccgcccg ggccgcgaac
     7801 cgcgaggacc cctccggcgc ggaccgtctc gtggagcgcg cccgacgccc gtccctggcg
     7861 gcgcaccgag atctcgaggt accccgacgc ctccggcgcg gagctgatcg aatagcagcg
     7921 gacctcctcc cggccgcccg ccgggacgcg aacgatcagg aactgcccgg cccggaacgc
     7981 gaagcccgcc ggcctctcca cccggaacgt ccggatctcg ggcgtctcgt cgacgacgga
     8041 gagcaccttc accggaacga agccggcccg gggccccggc tcgcccggcg ccgcggcgcc
     8101 cggggacgcg gcgcggctcc ggggcgcggc cggtccggcc ggagccgcct cgtcgcgacc
     8161 gccgccgagg agcttctcgc acgccacgac ccacgcggcc tgcgccgcga ggcccgcgat
     8221 aagccacgcc ggcgcgggga ggagccaggc gaagacgggg agcgcgatcg agggaaggag
     8281 gagggcgcgg cgcccctcgc ccgcccgccg gtgggcgagg aggaggaccg ccgcgaggtg
     8341 cagcgtcgtg atgaacgcga cggcggcgac gggagcgggc gaagccacgg tgcgcggagg
     8401 atagcggccc tccctggccc cgcccgaacg gcgcccgaac ccgttcgcgc cgcgccctca
     8461 ggccgtcgcg ccggctcccg cgtccccctt cggcggcacg aacgtcatcg cccgctcggc
     8521 gagcgccgtg atcgtcagcg acgggttcac gcccgggttc gcggagatcg ccgagccgtc
     8581 gacgacccag aggccctcgt agccgaagac gcggtggcga tggtcgatga cgccgttctc
     8641 cggggaatcg cccatcgggc agccgccgag gatgtgcgcc gtcgtcggga tgccgaggag
     8701 cgtctccgtg aggatgctct gcgggaagcc gtcgagcgcc tcgcaggcgc gtttcgtcag
     8761 gtcgttcgcc tccggatgcc acgccttcgc ccccgcgccg ccgccggcca ccgaggagag
     8821 gccgcggcgg aacccggtcc gccacgagcg gccgagcgtc atcctcaggt gcccgtccac
     8881 cgtccgcatg tagaggagga tcatcgtccg cttcgcccag tcggggaccc agaacgactt
     8941 cgtcgccttc cacgggctcg cgaggatcgt cgagaaggcg tgggcgaagc gctggaagag
     9001 cgtgtcgccc ggcgcgaacg ggagggcgag gaggcggagg aagcccgagc ccttcgagta
     9061 gcggaccggc tcgaggtgcg agtgctcgtc ggtgtggagg atcgagccga tcgcgacccc
     9121 ctgcgagagg tcctcgtcct tcgtcgtcac gacgccgacc aggacctccg agttcgtcct
     9181 cacgaagtcg ccgaccctcg gcgagagcgc gggaaggcct tcggggctct ctttcagctc
     9241 gaggagcagc ttgaccgtcc cgaggacgcc gcccgagaag acgacgttcc gagccgtgat
     9301 ccgctcctcc ttccggagcg ccgggaccgg cgcgccgccg acgcgcgccg acagctcgta
     9361 gccgccgccc gggagcggct tcacccaggt cacttcggtg tcggccttca cgacggcgcc
     9421 gagcccctcg gcgaggaaga ggtagttccg gtcgagggtg ttcttcgcgc cgtgccggca
     9481 gccgaccatg cagccgccgc agcgcgtgca gacggtccgc tccggcccct tccccccgaa
     9541 gaacgggtcg gggaccgtgt cgcccggctt gtccccctgc ccctcgcgtc cgaagaagac
     9601 cgcgacgttc gtcgactcga aaccgtcccc gcgcccgatc tgcctcccga tgtcgcggat
     9661 cacctcgtcg ggcttcgtca ggaacggcgt ccgcgccgcc ccgagcatcg cgagcgccgt
     9721 ccggtagtgg ggcgcgagct cctcttccca gcgcgcgagc cgcgcccacg aaggcgcctc
     9781 gaagaagggc ttcttcggga cggggagcgt gttcgcgtag acgagcgagc cgccgccgac
     9841 gccgacgccg gagagagcg
//
LOCUS       DAOTEZ010000132         9743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4931_length_9743_cov_5.962221, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000132 DAOTEZ010000000
VERSION     DAOTEZ010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9743
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4931_length_9743_cov_5.962221"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="P5164_08605"
     CDS             complement(<1..345)
                     /locus_tag="P5164_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002466848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43990.1"
                     /translation="MSATPTAAPRPERPRLSVLGEKALETLIRLAGVSAMLFVAAIFF
                     FVLREAFPVLATEKFSLTQFLFSTEWYPTSAKNVRYGTFALTIGTLSVTALAILIAVP
                     FGLGAAIYLSEFC"
     gene            complement(342..1304)
                     /locus_tag="P5164_08610"
     CDS             complement(342..1304)
                     /locus_tag="P5164_08610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRY43991.1"
                     /translation="MPRAAFARPLLLLAAVAALGLPACGRRDGGASPSNVIQNKGSDT
                     MVNVAQVWAEAYRKAAPDVEVEVSGGGSGVGIAALVKGAVDIANASRDIKPSESEMAL
                     ANSGKAPVDFKVGYDALAVFVHRDNPVSELTLAQLKEIYAEGGRALRWSDLGVTIPGV
                     SDDRIVRVSRQSSSGTYEFFRSRALDDEDFRLGSLDLNGSKEVVELVAVTPTAIGYSG
                     MGYATDHVKMLRVSPGAGVPGVGPTVDAVHDKTYPLARSLHLYTLGPPEGAVKDYIDW
                     ILSPAGQEVVRVAGYVPAVVAPAPPASAAPAAPAPPAPEAAPAR"
     gene            complement(1329..2435)
                     /gene="phoU"
                     /locus_tag="P5164_08615"
     CDS             complement(1329..2435)
                     /gene="phoU"
                     /locus_tag="P5164_08615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRY43992.1"
                     /translation="MPTRLEESLGRDIDRIRQQLLEMSALAERNLRDCVEAMKGGERA
                     RAYAVILRDQLIDEKEKEIDRLCLEFLVRQQPVGFNLRLAYSAIKINLELERVGDYAE
                     SIARQVLRLEKLPATPETHTGRLVEMADLAIPMIHDATRAFLAQDADLARAVVAREPA
                     VDALLGTLVTELLDEVSAGRIGAEMLVALSTIGRRFERVADQARNVCMETLYMCTGQY
                     AKHPGAETFRVLFVGEHNACRSQMAEAIGQSLDQSRFLFSSAGLSPRAIDPRTAEFLT
                     EKGYDAARMAPKGTDQVPNLDYYNVIVGVAPEAQSLFPRTPRNLVYLDWSTADPSRAA
                     GSPEEVRAAYQATYEFLERNIRDLVGAVLDGTHD"
     gene            2629..>2901
                     /locus_tag="P5164_08620"
     CDS             2629..>2901
                     /locus_tag="P5164_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRY43993.1"
                     /translation="MTDPRADIGLIGLAVMGENLVLNFESRGFRVAVHNRTVERVDAF
                     LAGRAKGRNVVGARSVAELVAALKTPRRVMLMVKAGPAVDGLIDALV"
     assembly_gap    2902..3001
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3002..3889
                     /locus_tag="P5164_08625"
     CDS             <3002..3889
                     /locus_tag="P5164_08625"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416288.1"
                     /note="catalyzes the formation of D-ribulose 5-phosphate
                     from 6-phospho-D-gluconate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HRY43994.1"
                     /translation="KMVHNGIEYGDLQLLGEAYHLMERLLGMGAAEMSGVFDGWSRGD
                     LDSYLVEVTRDVLAARDADGRPLVDAILDVAGQKGTGRWTSATALELGVPLGLIDTAV
                     SARFLSTLKEERVAAAKLLPGPAPSFAGDRAAFVDDLGKALFAAKLVSYAQGFALMRA
                     ASESRGWGLRFGGIALLWRAGCIIRSSFLDRVKEAYEAEPALPNLLLDPWFRGKVGEA
                     QDAWRRVVSAAALSGVWAPGLSAALAWYDGYRSARLPANLLQAQRDYFGAHRYERVDR
                     PRGELFHTEWGAGSGSGRD"
     gene            complement(3899..5833)
                     /locus_tag="P5164_08630"
     CDS             complement(3899..5833)
                     /locus_tag="P5164_08630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HRY43995.1"
                     /translation="MNGFPLLPDAEWLETDGLGGYALSTVAGRNARRYHGLLVSATTP
                     PTGRAVLLAKLEETLVVGGERFDLSVNRYSDGKLHPRGDLLLRSFRVDPFPTWEYEAG
                     GARLRRTFFLAHGEPTAVVTWELLSGDGLPVRLEVRPLLAYRDHHALRGAGTPCDPAL
                     AREDGAVTLTPVSGLPPLRLAHDAASAAETFFWYRGFEYELDRARGFEYQEDLFSPVA
                     FGWELSERPSAALVATLGDRSASDASDLAEAEERRRRKVEGAGSDRRALRLLRSAADR
                     FVARRGDGWTIVAGYPWFTDWGRDAMIALPGLLLATRRFEAARSVLETFARHVDRGMV
                     PNRFPDEGTEPEYNNVDGTLWFVEAVRAYYQATRDDAFRRHVYPLLVEIVERHLRGTR
                     FGIRCDVDGLLTSGEPGQQLTWMDAKVDGRVVTPRAGKPVEVQALWHNALRVVSLYAA
                     DLGDAFTAARLLAIADQARESFSALFWDERLGWLADVVGTDGTRDLSLRPNQLFALSL
                     HRPLLTGRRAERALLAIEERLLTPYGLRTLDPAHPDYRGRFEGGPAERDAAYHQGSAW
                     PWLLGPFVDAHLAVRGRSPESRRRASAWLAPLLEHLLGPGLGQLPEVFDGDPPHRAGG
                     CVAQAWSVGEVLRAALKTGG"
     gene            complement(5886..6260)
                     /locus_tag="P5164_08635"
     CDS             complement(5886..6260)
                     /locus_tag="P5164_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY43996.1"
                     /translation="MRATCRLALLLFAALAAAGCGCVWEGRNVWLATEASAGKTLEAN
                     ARDEVLLELPVDAGSKARWYVVEVDEKVLRRKGPAEEQSDGTVRIPFEALRTGVTDVV
                     AFYATARSAPPERTFRTTVWVR"
     gene            6357..7190
                     /locus_tag="P5164_08640"
     CDS             6357..7190
                     /locus_tag="P5164_08640"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRY43997.1"
                     /translation="MTPPERLSFRHRVVAGDPPGAADLLARVSGLSKGVVKDAMRKGA
                     VHVGRAGRTPNRLRRETATVRPGDVLEIHYDAALLALVPPEARLVRDATRWSAWDKPA
                     GLLTQGTPFGDHATLLRQAEKALGRPAFPVHRLDREVSGLVLVAHDERAAAALSALLR
                     EGRVVKRYRAEVLGDVASALGREGVVETPLDGKSARTLWRVVAHDAERGATTLDVTIE
                     TGRPHQIRRHLESIGHPVLGDPRYGAGNKNRDGMRLAAVFLAFAEPFTGEAVELALGP
                     G"
     gene            complement(7214..8506)
                     /locus_tag="P5164_08645"
     CDS             complement(7214..8506)
                     /locus_tag="P5164_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017941197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HRY43998.1"
                     /translation="MGGEERSFLKALGPGLLWAGAAVGVSHLVQSTRAGASFGLGLVV
                     VVLAANFFKYPAFQFGPRYAAATGTSLLEGYRRQGRWALVLYAVLTAGTMFTVQAVVT
                     LVTAALFAALLGWSSPFLGLPAPFWLAVLLLGLCAGLLLVGRYRWLDRLNKVVVLVLT
                     LTTVAATLLVLPKVPWRSIPLLPDASAFTDPRSVAFLVALVGWMPSAFDISIWHSLWT
                     LARREQTGHAPRVKEAVLDFDIGYLGTVLLALCFVLLGAGVMYGSGEAFDDRAAAFAQ
                     QVIALYTRNLGAWAAPLIGLAAGTVMFSTTLTVADGFPRALSGLAARFRGPERPGEAT
                     VRDRAYWGALLVLAAGSLAIIGYALRGGAAFKALVDLATSLSFLTAPALAWLNHRAIH
                     AAEVPAEHRPSRALTAWSVSGILFSTAFAAWYLKVLAG"
     gene            8650..9267
                     /locus_tag="P5164_08650"
     CDS             8650..9267
                     /locus_tag="P5164_08650"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRY43999.1"
                     /translation="MKVTLRSERVVSDENAKKETGRGFAGWWTLLDAFGGPAKGRREI
                     GNHLVNELKVDPWWSATLQVEYERHKGLVEKDGKGKGYTICATKSLKAPPERCWELFA
                     TGKALDGWFGPENVFDLKPGGSLSNADGNAADVRGVTPGKTIRLVWKDASAPGTPVEV
                     KLQPAAGKTTVMVTHDRLQTREEADGLRAAWGEALDRLKKLVEAG"
     gene            complement(9359..>9743)
                     /locus_tag="P5164_08655"
     CDS             complement(9359..>9743)
                     /locus_tag="P5164_08655"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003232308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HRY44000.1"
                     /translation="LRNDPDRVLDAYFLLCSISMSCSELASAFQYLATGGLSAGGEEV
                     ATARQVKRVNAVMLTCGVYDEAGDYAYRVGLPAKSGVGGGVVAVMPGRFVTAVWSPGL
                     SEKGNSLAGTMALELLTTKTSLSVF"
BASE COUNT         1405 a         3325 c         3714 g         1199 t
ORIGIN      
        1 gcagaactcg gagaggtaga tcgcggcccc gaggccgaac gggacggcga tgaggatcgc
       61 gagcgcggtg acgctgagcg tcccgatcgt cagcgcgaag gtgccgtagc ggacgttctt
      121 cgccgaggtc gggtaccact cggtcgagaa gaggaactgc gtgagggaga acttctcggt
      181 cgcgaggacg gggaaagcct cgcgcaggac gaagaagaag atggcggcga cgaagagcat
      241 cgcgctcacg ccggcgaggc ggatcagcgt ctcgagcgcc ttctcgccga ggacggacag
      301 gcgggggcgc tccgggcggg gcgcggcggt cggcgtggcg ctcatcgtgc gggggcggcc
      361 tccggagctg gcggtgcggg cgcggccgga gccgcggagg cgggcggagc gggagccacg
      421 acggcgggaa cgtaccccgc cacgcgaacg acctcctggc cggcggggga gaggatccag
      481 tcgatgtagt ccttcaccgc gccctcgggc ggcccgagcg tgtagaggtg gagggaccgc
      541 gcgaggggat aggtcttgtc gtggacggcg tcgaccgtcg gcccgacgcc cggcacgccc
      601 gccccgggcg agacgcggag catcttcacg tggtccgtcg cgtagcccat gccgctgtag
      661 ccgatcgcgg tcggcgtcac cgcgacgagc tcgacgacct ccttcgagcc gttcaggtcg
      721 agggagccga gccggaagtc ctcgtcgtcg agggcccggc tccggaagaa ctcgtaggtc
      781 ccggagctcg actgccggct cacgcggacg atccggtcgt ccgaaacgcc ggggatcgtg
      841 acgccgaggt ccgaccagcg gagggcgcgc cccccttcgg cgtagatctc cttcagctgc
      901 gcgagcgtca gctcggagac ggggttgtcg cggtggacga agacggcgag ggcgtcgtag
      961 ccgaccttga agtcgaccgg cgccttcccc gagttcgcga gcgccatctc ggactcgctc
     1021 ggcttgatgt cgcggctggc gttcgcgatg tcgaccgccc ccttcacgag cgccgcgatc
     1081 ccgacccccg agccgccgcc ggagacctcg acctccacgt cgggcgcggc cttccggtac
     1141 gcctcggccc agacctgggc gacgttgacc atcgtgtcgg agcccttgtt ctggatcacg
     1201 ttcgagggag acgcgccccc gtcgcggcgt ccgcaggcgg ggagcccgag cgccgcgacg
     1261 gcggcgagga ggaggagcgg tcttgcgaat gcggctcggg gcatgggctc cgggcgcggc
     1321 tcccgccgtc agtcgtgcgt gccgtcgagg acggcgccga cgaggtcgcg gatgttccgt
     1381 tcgaggaact cgtaggtggc ctggtaggcg gcgcggacct cctcggggga gcccgcggcg
     1441 cgcgacgggt cggcggtgct ccagtcgagg tagacgaggt tgcgcggcgt gcgggggaag
     1501 aggctctgag cctcgggcgc gacgccgacg atgacgttgt agtagtcgag gttcgggacc
     1561 tggtccgtcc ccttcggggc catgcgcgcg gcgtcgtagc ccttctccgt gaggaactcg
     1621 gccgtccgcg ggtcgatcgc gcgcggcgag aggccggcgc tcgagaagag gaagcggctc
     1681 tggtcgagcg actgcccgat cgcctcggcc atctggctcc ggcaggcgtt gtgctcgccg
     1741 acgaagagga cgcggaacgt ctcggcgccg gggtgcttcg cgtactgccc ggtgcacatg
     1801 tagagcgtct ccatgcagac gttccgggcc tggtcggcga cccgctcgaa gcggcgcccg
     1861 atcgtcgaga gcgcgacgag catctcggcc ccgatccgcc ccgcgctcac ctcgtcgagg
     1921 agctcggtga cgagcgtccc gaggagcgcg tccaccgccg gctcgcgcgc gacgacggcg
     1981 cgggcgaggt cggcgtcctg ggccaggaac gcccgggtgg cgtcgtggat catcgggatc
     2041 gcgaggtcgg ccatctcgac gaggcgcccg gtgtgggtct cgggcgtcgc cgggagcttc
     2101 tcgaggcgga gcacctggcg ggcgatgctc tcggcgtagt ccccgacccg ctccagctcg
     2161 aggttgatct tgatcgcgct gtaggcgagg cggaggttga agccgacggg ctgctgccgc
     2221 acgaggaact cgaggcagag ccggtcgatc tccttctcct tctcgtcgat cagctggtcg
     2281 cgcaggatga cggcgtaggc gcgggcccgc tcgcccccct tcatcgcctc gacgcagtct
     2341 cggaggttcc gctcggcgag ggcggacatc tcgaggagct gctggcgaat ccggtcgatg
     2401 tcgcgtccga gggactcttc gaggcgagtc ggcatccttc tccccggcga aagaggatcg
     2461 ctcctccccg ctatctcctt gcagcggcga accataacag aggacgggag ggagtcaaac
     2521 gaagcggagg aaacggcggc ggccccggcg cgaaggccgc ggcacggctc gagcgccgaa
     2581 tgggggatca tcgaggagag ccggcgcacc ggcccgagga ggcgagggat gacggaccca
     2641 cgcgcggaca tcggcctgat cggcctcgcc gtcatgggcg agaacctcgt cctcaacttc
     2701 gagagccggg ggttccgggt ggccgtccac aaccggacgg tcgagcgcgt cgacgcgttc
     2761 ctcgcggggc gggcgaaggg gcggaacgtc gtcggggcgc ggtcggtcgc cgagctcgtc
     2821 gccgcgctga agaccccgcg ccgggtgatg ctgatggtga aggcgggccc ggccgtcgac
     2881 gggctgatcg acgccctcgt cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 naagatggtc cacaacggga tcgagtacgg cgacctgcag ctcctcggcg aggcctacca
     3061 cctcatggag cgcctcctcg ggatgggcgc cgcggagatg agcggggtct tcgacggatg
     3121 gagccggggc gacctcgaca gctacctcgt ggaggtgacg cgcgacgtcc tcgcggcgcg
     3181 cgacgccgac ggacgcccgc tcgtcgacgc gatcctcgac gtggcggggc agaaggggac
     3241 ggggcgctgg acgagcgcca ccgcgctcga gctcggcgtg ccgctcggcc tgatcgacac
     3301 ggcggtctcg gcccggttcc tctcgacgct gaaggaggag cgggtcgccg ccgcgaagct
     3361 cctcccgggc ccggcgccgt ccttcgccgg cgaccgcgcg gcgttcgtgg acgacctcgg
     3421 gaaggcgctc ttcgccgcca agctcgtctc ctacgcgcag gggttcgccc tgatgcgggc
     3481 ggcttccgag agccgcggct ggggccttcg cttcggaggg atcgcgctcc tctggcgggc
     3541 gggctgcatc atccggtcgt ccttcctcga ccgcgtgaag gaggcttacg aggcggagcc
     3601 ggcgctcccg aacctcctcc tcgacccctg gttccgcggc aaggtcgggg aggcccagga
     3661 cgcctggcgc cgggtcgtct cggcggcggc gctctcgggc gtctgggctc ccggcctctc
     3721 ggccgccctc gcctggtacg acgggtaccg gagcgcgcgc cttcccgcga acctcctcca
     3781 ggcgcagcgc gactacttcg gcgcgcaccg ctacgaacgg gtcgaccgcc cgcgcgggga
     3841 gctgttccac accgagtggg gagccgggag cggctccggc cgggactgac cggcccgctc
     3901 agccgcccgt cttcagcgcg gcgcggagca cctcgccgac gctccaggcc tgggccacgc
     3961 agccgcccgc gcggtggggc gggtctccgt cgaagacctc cggaagctgg ccgagccccg
     4021 ggccgaggag gtgctcgagg aggggcgcga gccaggccga ggcgcgccgg cggctctcgg
     4081 gcgacctccc tcggacggcc aggtgcgcgt cgacgaaggg tccgaggagc cagggccagg
     4141 cgctcccctg gtgatacgcc gcgtcccgct ccgccggacc gccctcgaag cggcctcggt
     4201 agtcggggtg agcggggtcg agggtgcgca ggccgtacgg ggtgaggagc ctctcctcga
     4261 tcgcgaggag cgccctctcg gcccgccgac cggtgaggag gggcctgtgg agcgagaggg
     4321 cgaagagctg attggggcgg agggaaaggt cgcgcgtccc gtccgttccg accacgtcgg
     4381 cgagccagcc gaggcgttcg tcccaaaaga gcgccgagaa gctctcccgc gcctggtcgg
     4441 cgatcgcgag gaggcgggcc gccgtgaacg cgtcgccgag gtcggcggcg tagaggctga
     4501 cgacccgcag ggcgttgtgc cagagcgcct gcacctcgac cggcttgccg gcgcgcggcg
     4561 tgacgacgcg gccgtcgacc ttcgcgtcca tccaggtgag ctgctggccc ggctcgccgg
     4621 acgtcaggag accgtccacg tcgcagcgga tgccgaagcg cgtgccgcgc aggtgccgct
     4681 cgacgatctc gacgagcagc gggtagacgt gccgccggaa cgcgtcgtcc cgcgtcgcct
     4741 ggtagtacgc gcggaccgcc tcgacgaacc agagcgtgcc gtcgacgttg ttgtactcgg
     4801 gctcggtccc ctcgtcgggg aagcggttcg ggaccatccc gcggtcgacg tggcgggcga
     4861 acgtctcgag gaccgagcgg gccgcctcga accggcgtgt cgcgaggagg aggccgggga
     4921 gagcgatcat cgcgtcgcgc ccccagtcgg tgaaccacgg gtagccggcg acgatcgtcc
     4981 agccgtcgcc gcgcctggcg acgaagcggt cggcggcgct ccggaggagg cgcagggcgc
     5041 ggcggtccga gccggctccc tcgaccttcc gccgccgccg ctcttccgcc tcggcgaggt
     5101 cggaggcgtc ggaggccgac cggtcgccga gggtcgcgac gagcgcggcc gacggccgct
     5161 ccgagagctc ccagccgaac gcgaccggcg agaagaggtc ctcctggtac tcgaagccgc
     5221 gggcgcggtc cagctcgtat tcgaagccgc ggtaccagaa gaaggtctcg gcggcggagg
     5281 ccgcgtcgtg ggcgaggcgg agcggcggga gaccgctgac cggcgtgagc gtcacggcgc
     5341 cgtcctcccg ggcgagcgcc gggtcgcacg gcgtcccggc gccgcggagg gcatggtggt
     5401 cgcgataggc gaggagcggc cggacctcca gccggacggg gaggccgtcg ccggagagga
     5461 gctcccacgt cacgaccgcc gtcggctcgc cgtgcgcgag gaagaaggtg cggcgcaggc
     5521 gcgcgcctcc cgcctcgtac tcccacgtcg ggaacgggtc gacgcggaac gagcggagca
     5581 gcaggtcgcc gcgcgggtgg agcttgccgt ccgagtagcg gttcaccgag aggtcgaacc
     5641 gctccccgcc gacgacgagc gtctcctcga gcttcgcgag gaggacggcg cggccggtcg
     5701 gcggcgtcgt ggccgagacc aggaggccgt ggtagcggcg cgcgttccgc ccggccaccg
     5761 tcgacagcgc gtagccgccg agaccgtcgg tctcgagcca ttcggcgtcg gggaggaggg
     5821 ggaagccgtt catcgtcgtg gaagtcgcgg aggaacgtta caccgggtcc ccccgggcgc
     5881 gcacctcaac ggacccacac ggtcgtccgg aacgtccgct cgggaggcgc gctccgcgcc
     5941 gtggcgtaga aggcgacgac gtccgtgacc ccggtgcgca gcgcctcgaa cgggatgcgg
     6001 accgtcccgt ccgactgctc ctccgccggg cccttcctgc ggaggacctt ctcgtcgacc
     6061 tccacgacgt accagcgcgc cttcgacccg gcgtccacgg ggagctcgag gagcacctcg
     6121 tcgcgggcgt tcgcctcgag cgtcttcccc gccgaagcct cggtggcgag ccagacgttc
     6181 ctcccctccc agacgcagcc gcagccggcg gccgcgagcg cggcgaagag caggagcgcg
     6241 aggcggcacg tggcacgcat ggcgtcgatt ccctccggac ggtaccgggg cgatgccgca
     6301 atcgtacgcc ggcggcgctc cgcagggcac cggctcccgc gctatcgtcg ggaccgatga
     6361 ctccgcccga gcgcctctcc ttccggcacc gcgtcgtcgc gggcgaccct ccgggcgcgg
     6421 ccgacctcct cgcgcgcgtc tcgggcctct cgaagggggt cgtgaaggac gcgatgcgga
     6481 agggggcggt ccacgtcggc cgcgcggggc ggacgccgaa ccgcctccgc cgcgagacgg
     6541 cgaccgtgcg tcccggcgac gtcctcgaga tccactacga cgccgccctc ctcgcgctcg
     6601 tgccgcccga ggcgcgcctc gtccgcgacg cgacgcgctg gtcggcgtgg gacaagcccg
     6661 cggggctcct gacgcagggg acgccgttcg gcgaccacgc gacgctcctc cggcaggcgg
     6721 agaaggcgct cggccgcccc gcgttccccg tccacaggct cgaccgcgag gtctccgggc
     6781 tcgttctcgt cgcgcacgac gagcgggccg ccgcggcgct ctccgcgctc ctgcgcgaag
     6841 ggcgcgtcgt caagcgctac cgcgccgagg tcctcggcga cgtcgcctcc gcgctcggcc
     6901 gcgagggcgt cgtcgagacg ccgctcgacg gcaagagcgc ccggacgctc tggcgcgtcg
     6961 tcgcgcacga cgcggagcgc ggcgcgacga cgctcgacgt gacgatcgag accggccgtc
     7021 cgcaccagat ccgccggcac ctggagtcga tcggccaccc cgtcctcggc gacccgcgct
     7081 acggcgccgg gaacaagaac cgcgacggga tgcgcctcgc cgcggtcttc ctcgcgttcg
     7141 cggagccgtt cacgggggag gccgtcgagc tggcgctcgg gccgggctga tcgcccggcc
     7201 ccggccggcc gaatcagccg gcgaggacct tcaggtacca ggcggcgaac gcggtcgaga
     7261 agaggatccc cgaaacgctc cacgcggtca gcgcgcggga cggccggtgc tcggccggga
     7321 cctccgccgc gtggatcgcc cggtggttca gccacgcgag cgcgggagcc gtcaggaacg
     7381 agagcgacgt cgcgaggtcg acgagggcct tgaacgccgc cccgccccgg agcgcgtacc
     7441 cgatgatcgc gagggagccg gcggccagga cgagcagcgc gccccagtag gcccggtcgc
     7501 ggaccgtcgc ctcgcccggg cgctccggcc cgcggaagcg ggcggcgagg cccgagaggg
     7561 cgcgcgggaa gccgtcggcg acggtgagcg tcgtcgagaa catcacggtg cccgccgcga
     7621 gcccgatgag gggcgctgcc cacgcgccga ggttccgcgt gtagagcgcg atgacctgct
     7681 gcgcgaacgc cgccgcgcgg tcgtcgaacg cctcgccgga gccgtacatg acccccgcgc
     7741 cgaggaggac gaagcaaagc gcgaggagga ccgtgccgag gtacccgatg tcgaagtcga
     7801 ggacggcctc cttcacccgc ggcgcgtgcc ccgtctgctc gcgccgggcg agcgtccaga
     7861 gcgagtgcca gatcgagatg tcgaacgccg agggcatcca gccgacgagg gccacgagga
     7921 acgcgaccga gcgggggtcg gtgaacgcgg aggcgtcggg gaggaggggg atgctccgcc
     7981 acgggacctt cgggaggacg agcagcgtcg ccgcgacggt cgtgagcgtc aggacgagga
     8041 cgacgacctt gttgagccgg tccagccagc ggtagcgccc gacgaggagg agcccggcgc
     8101 agaggccgag gagaaggacg gccagccaga acggcgccgg gagcccgagg aacggggagg
     8161 accagccgag gagcgcggcg aagagagccg ccgtgacgag cgtgaccacg gcctggaccg
     8221 tgaacatcgt cccggccgtc aggacggcgt agaggacgag ggcccagcgg ccctggcggc
     8281 ggtacccctc gaggaggctc gtcccggtgg cggccgcgta ccgcgggccg aactggaacg
     8341 ccgggtactt gaagaagttg gctgccagga cgacgaccac gagtccgagg ccgaagctcg
     8401 ccccggcccg ggtgctctgg acgaggtgcg agacgccgac cgcggctccg gcccagagga
     8461 ggccggggcc gagggccttc aggaacgacc gttcttcgcc gcccatgaat gcggattctc
     8521 gtctcggggg aaccccgcga caacgtcctt cgggacggcg agggaccggt tccgccgagg
     8581 tttgcgattg cgcggcggca agcgcaaaga tccgcgccgt tccgttgaag gggaaggcag
     8641 gaggaaccga tgaaggtgac gcttcggtcc gagagagtcg tgtccgacga gaacgcgaag
     8701 aaggagaccg gcaggggctt cgccgggtgg tggacgctcc tcgacgcctt cggagggccg
     8761 gcgaagggcc ggagggagat cggcaaccac ctcgtcaacg agctgaaggt cgacccctgg
     8821 tggtcggcga cgctccaggt cgaatacgag cggcacaagg ggctcgtcga gaaggacggc
     8881 aaagggaagg gctacacgat ctgcgccacg aagtccttga aagcgccgcc ggagaggtgc
     8941 tgggagctgt ttgcgaccgg caaggcgctc gacgggtggt tcggcccgga gaacgttttc
     9001 gacctgaagc ccggcggctc cctttcaaac gccgacggga acgccgccga cgtaagaggg
     9061 gtgacgccgg ggaagacgat ccggctcgtc tggaaggacg ccagcgcgcc cggcacgccg
     9121 gtggaggtga agctccagcc ggccgccggc aagacgaccg tgatggtgac gcacgaccgg
     9181 ctccagacgc gcgaggaggc cgacggcctg cgcgccgcgt ggggcgaggc gctcgacagg
     9241 ctgaagaagc tcgtcgaggc gggctgaacg tgttccgcca ggcctgcgcc ctcctcctcg
     9301 actccgtgcc ggtagcgcgg aggctcgcgc cgtagcgggg tccggaagcg cgaccgcctc
     9361 agaagaccga aagagaggtc ttcgtcgtca gcagctcgag cgccatcgtc ccggcgaggg
     9421 agttgccctt ctcggagagg ccgggcgacc agaccgcggt cacgaaccgg ccgggcatca
     9481 cggcgacgac gccgccgccg acgccgctct tggccgggag gccgacccgg tacgcgtagt
     9541 ccccggcctc gtcatagacg ccgcacgtca gcatcaccgc gttcacccgc ttcacctgcc
     9601 gcgcggtcgc gacctcctcg ccgccggccg agagcccgcc cgtcgccagg tactggaacg
     9661 cggacgccag ctcggagcag ctcatcgaga tcgagcagag gaggaagtag gcgtcgagga
     9721 cgcggtccgg gtcgttcctc agg
//
LOCUS       DAOTEZ010000133         9730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4940_length_9730_cov_6.577571, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000133 DAOTEZ010000000
VERSION     DAOTEZ010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4940_length_9730_cov_6.577571"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1622)
                     /locus_tag="P5164_08660"
     CDS             complement(<1..1622)
                     /locus_tag="P5164_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY44001.1"
                     /translation="MMREAFYSTVRGAADVAWKGFQWVNRRVPARSFHPAWSPEPLPK
                     SHERTKPPLGFPRRTDSLCPVCTREVREAIVKGQEDLKALIDGHPGEIPADIVQRGQE
                     IWMVKTCPKHGTFEDLMSNDVDFYRRLEGNFFGRDVKIGKDALHEHGSSSMRYGRGAV
                     LTVDLTNRCNMMCNPCFMDANQVGYVHELTWDDVKQILDNAASIKPRRQLSVQFSGGE
                     PTLSPHFLEAIRYSKEIGYFAVQCASNGIRFAQEPGFAKAAAEAGLRLVYLQFDGVGN
                     EHNQHRGVGNLYDVKLRALENLKAAGVDVTLVITIVNSINNDQVGDIIRFAIRNIDKI
                     NALSFQPVSFTGRDEEIDDETRMRQRYTLSHLASDVKTQAGLGEPMRDWFPLSASGPF
                     SDLRDLLGGLEAEWGSLKCGCHPNCGIATMVLVHEKTGDAVPVTQLLDVDRLLRDLTV
                     ITDAARGRFLTVLQAVLATARNIRPAGVPKNLGVLETLRIMDGHTGKSMGIAKSGRYP
                     WRVLLVAGMWFQDLFNYDFRRTEMCIIPYGTQL"
     gene            complement(1719..2843)
                     /locus_tag="P5164_08665"
     CDS             complement(1719..2843)
                     /locus_tag="P5164_08665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY44002.1"
                     /translation="MTRTLSSADVVVCGPGLAAAAVALELARRNLDVVLLAPGRVGRP
                     VGPGAPVAAQAFVEEATPEAGGRLALFARDLFPDWLSGLEEETGVSGELDQRGALTVA
                     FDEPEEVLLDRALDAQRRRGMPFEVLEPEEARGREPALAPELRAAFSFPRDGVARARR
                     VGRALVLAGRGAGVRLIEGTGARVVARTGGRVSGVETPAGFLPAAAVVLAEGEAVERG
                     EAGLPPFPLEASVRPWLRLDASSDPDRPGRLLVSSGVRLVPRRDGTLVALGRPGRDGR
                     TARCGAGRLAVLLEELRRLVPGSTAWEAGESGAALELRAPDGLPLAGETDVAGLFLAT
                     GWGSDELLLAPAAARLLGDLVAGKEPPVPAAPYAPGRYGL"
     gene            complement(2840..3439)
                     /locus_tag="P5164_08670"
     CDS             complement(2840..3439)
                     /locus_tag="P5164_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44003.1"
                     /translation="MKIAESFLALVRRASTDLDVERIRDGVRELSRRHPDLSTRRKAA
                     LMVESSARKAALVGAAASVPPGWAAVAATAPELTTLIVLQSRMIVGLHLLYGAELDPE
                     ERALEVVAGLAAGAGLSVGRRLTVGLAESLATRLAARSAGRPLAHLIPLAGIAASAVL
                     NYAAVRAVGRAVVVRVERRWGPPELAGRGPVLAAEGRVE"
     gene            3669..4238
                     /locus_tag="P5164_08675"
     CDS             3669..4238
                     /locus_tag="P5164_08675"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRY44004.1"
                     /translation="MVSSTPRDYAKTLAVLKTSQGDVTIRFFFDKAPGHVKNFVDLAA
                     RGFYDGTLFHRVIPGFMVQGGDPLTKDPSKSALFGTGGNTDKAGKPVNVKAEFNDVSH
                     KRGILSMARASDPHSASSQFFLVVQDSPFLDRQYTAFGEVVKGLEVLDKLVTESNSDT
                     SDPRSGGKPRAYQKILKVELVAEAAPGNA"
     gene            4235..5167
                     /locus_tag="P5164_08680"
     CDS             4235..5167
                     /locus_tag="P5164_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRY44005.1"
                     /translation="MTLPAPAGQEARVPSACSRFGLPESGPFQLLAGDVGQRRYVRLR
                     TWADATVVLVLYPEPSSPAQERWRRIGEALAREGLRVPALLDDDPASGTALVEDLGDR
                     DLAADLREAAPDERARLLEEAEELLAPLRGMAPEAALLNPPFDAAFLASELGHTRRWA
                     LEGDGARPLAPERAAEWEELAARLAREAADASRVGGPVPTHRDFHANNLMRTPEGALA
                     LIDFQDLRLGHPDYDPVSLRFERAGALVSSSGGDRSEAVLLQRAWKVLGTFEKMLALG
                     REVYRPHRDAALRTIRERTRPDGPFAPLLAFLPG"
     gene            5200..5409
                     /gene="tatA"
                     /locus_tag="P5164_08685"
     CDS             5200..5409
                     /gene="tatA"
                     /locus_tag="P5164_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRY44006.1"
                     /translation="MPNLGLPELLVLLFIVVLLFGVGKLPQLGKGIGEGIRNFKQAVK
                     DGGQDSDKKEPPTGNGSGNGPSAPK"
     gene            5533..6444
                     /gene="htpX"
                     /locus_tag="P5164_08690"
     CDS             5533..6444
                     /gene="htpX"
                     /locus_tag="P5164_08690"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HRY44007.1"
                     /translation="MKIAKRVFLFLAVNVLVVATISVVLSLLGVRPYLNAYGLDLQAL
                     AVFCLVWGFGGAFISLALSRVMAKWGMGVKLVDPGTPDPGAQELVSTVHRLAQAAGLR
                     TMPQVGVYDSDEVNAFATGPTKNRALVAVSTGLLRRMRREEVEGVLGHEIAHVANGDM
                     VTMTLVQGVVNAFTMFLARVLAFFVAQLFRRGDEEGGGVSHLVYFVATLVFDIAFSIL
                     GSFVVAWFSRLREFRADAGGARIAGRDRMIGALEALQRTYGIVDPSDRQAAVQTMKIS
                     GHPKGLMRFLSTHPPLETRIARLKMLA"
     gene            complement(6620..6850)
                     /locus_tag="P5164_08695"
     CDS             complement(6620..6850)
                     /locus_tag="P5164_08695"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY44008.1"
                     /translation="MLDGQLQRIIDELVSKGIPLETARKEFERKYVATAVTRASGNMG
                     QAAKSLGIHRNTLRAKISLLRLKPAPKRRDHA"
     gene            complement(6972..7850)
                     /locus_tag="P5164_08700"
     CDS             complement(6972..7850)
                     /locus_tag="P5164_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44009.1"
                     /translation="MKRAFVPAMVVLACSVISLSAAAAGPGGLREAAPVPVPQEAVEA
                     PAAPAVIEQAPTERTSTSAPFPVGWEDDVYCAGWIGVDEEPVTGRIVAAEYEDSRHMF
                     GVGDIIYSDVGARAGLMAGQEFQVIRPAHEVYRWGSYTATIGRFYETPARVRVVCVQD
                     DSAILEITESCEPTYLGDLLIPFEPIPIPLVRASAPLTQCDPPSGKVTGRIVEVKDRA
                     TPVGTDSVVFLDLGEEDGLYPGDFLTVYRARNDSGTIRTLLGEAAVLWTKGKTCVAKI
                     TSMVDSMRAGDFVELK"
     gene            complement(7953..8765)
                     /gene="bamD"
                     /locus_tag="P5164_08705"
     CDS             complement(7953..8765)
                     /gene="bamD"
                     /locus_tag="P5164_08705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HRY44010.1"
                     /translation="MAHRIHRMTAASVLLGLAVLGAGACSSSDPSRVTRELLTMSKED
                     AYAQGDALVTKRKFEMGRQYLRFVAENYANDPLGKQAALRLADSFLEEKSATGYAEAQ
                     ARFKDFRSRYPSHPRSDYALFRLAQVSDRQAEKPDRDQANTRLAAQSYSELVVNYPSS
                     PYAFEARARYKAILDLLAEHEYLVARFYYRRGAWQAARSRFEGLFAAFPEYAGMDKAL
                     YWAGQTERRLGRTEDARALFDRLRRDHPESRFLRKVPRLPETPVAATAATGL"
     gene            complement(8780..9508)
                     /locus_tag="P5164_08710"
     CDS             complement(8780..9508)
                     /locus_tag="P5164_08710"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRY44011.1"
                     /translation="MSGVLARILYALLLAGILGTAAWISFSRFVLGKAEEVPDLTGRS
                     VDEATALAAERGLRVVVDGSQEGFDEEIPAHRVRGQSPAAGMAVKAGQDLRVFLSLGP
                     RIIRTPDLAGLTARTAALALTREGLVAGAVSAVRLPGPQGVVAQGVVPGAPTEPETPV
                     DVLVNRGAPDVLYVMPDLIGRDFERVRLAFEARGFRLGGVKAQPYEGAAAGTILRQYP
                     LAGAPVGLRDTLSFVVASPEPQPG"
BASE COUNT         1379 a         3592 c         3401 g         1358 t
ORIGIN      
        1 agctgcgtgc cgtacgggat gatgcacatc tccgtccggc ggaagtcgta gttgaagagg
       61 tcctggaacc acatcccggc gacgaggagg acgcgccagg ggtagcggcc cgacttcgcg
      121 atccccatcg acttgccggt gtggccgtcc atgatccgga gcgtctcgag gacgccgagg
      181 ttcttcggca cgcccgcggg ccggatgttg cgcgccgtgg cgaggacggc ctggaggacc
      241 gtcaggaatc gtccgcgcgc cgcgtccgtg atgacggtca ggtcgcgcag gagccggtcg
      301 acgtcgagaa gctgcgtcac ggggaccgcg tcgccggtct tctcgtggac gaggaccatc
      361 gtcgcgatgc cgcagttcgg gtggcagccg cacttcagcg agccccactc ggcctcgagg
      421 ccgccgagga ggtcgcgcag gtcggagaag ggcccggacg ccgaaagggg aaaccagtcg
      481 cgcatcggct cgccgagccc ggcctgcgtc ttcacgtcgc tggccaggtg cgagagcgtg
      541 tagcgctggc gcatccgcgt ctcgtcgtcg atctcctcgt cccgccccgt gaaggagacc
      601 ggctggaagg agagggcgtt gatcttgtcg atgttcctga tcgcgaaccg gatgatgtcg
      661 ccgacctggt cgttgttgat cgagttgacg atcgtgatga cgagcgtgac gtcgacgccg
      721 gcggccttca ggttctcgag cgcgcgcagc ttgacgtcgt agaggttccc gactccccgg
      781 tgctggttgt gctcgttgcc gaccccgtcg aactggaggt agacgaggcg gagccccgcc
      841 tcggccgcgg ccttggcgaa tccgggctcc tgcgcgaacc ggatgccgtt cgacgcgcac
      901 tggacggcga agtagccgat ctccttcgag tagcggatcg cctcgaggaa gtggggcgag
      961 agcgtcggct cgccgcccga gaactggacg gagagctggc gccgcggctt gatggacgcg
     1021 gcgttgtcga ggatctgctt cacgtcgtcc caggtcagct cgtggacgta gccgacctgg
     1081 ttcgcgtcca tgaagcaggg gttgcacatc atgttgcagc ggttcgtcag gtcgacggtg
     1141 aggaccgcgc cgcggccgta gcgcatcgag gacgagccgt gctcgtggag cgcgtccttg
     1201 ccgatcttca cgtcccgccc gaagaagttc ccctcgaggc ggcggtagaa gtcgacgtcg
     1261 ttcgacatca ggtcctcgaa cgtgccgtgc ttcgggcacg tcttgaccat ccagatctcc
     1321 tggccgcgct ggacgatgtc ggccgggatc tcgcccgggt gcccgtcgat cagggccttc
     1381 aggtcctcct gccccttcac gatcgcctcg cggacctccc gcgtgcagac gggacagagc
     1441 gagtcggtcc tccgcggaaa cccgagcggc ggcttcgtgc gttcgtggct cttcggcaac
     1501 ggctcgggcg accacgccgg gtggaaggag cgggccggga ccctccggtt gacccactgg
     1561 aaccccttcc aggcgacgtc cgcggcacct cgcaccgtcg aatagaaggc ctcacgcatc
     1621 atttctcggg tcctccctga aaggcgacgc cagcccgctc cgcaggagcg gatcgccgcc
     1681 ttgcacactt agccggacag aatagcattc caggcgcctc agagcccgta gcggccggga
     1741 gcgtagggcg cggccgggac cggcggctcc ttccccgcga cgaggtcccc gaggagccgc
     1801 gccgcggcgg gcgccaggag gagctcgtcg ctcccccagc ccgtcgccag gaagaggccc
     1861 gccacgtccg tctctcccgc cagcggaagg ccgtccggcg cccggagctc gagggcggcc
     1921 ccggactcgc ccgcctccca ggccgtcgag cccggcacga gacgccgcag ctcctcgagg
     1981 aggacggcga ggcgccccgc gccgcaccgg gccgtccgtc cgtcgcgccc gggccggccg
     2041 agggcgacga gcgtcccgtc ccttcggggg acgagacgga ctccgctcga gacgagcagg
     2101 cggccgggtc ggtccggatc ggaggacgcg tccagccgga gccacggccg cacggacgcc
     2161 tcgagcggaa acggcggcag cccggcctcg ccgcgctcca ccgcctcgcc ctccgcgagg
     2221 acgaccgcgg cggccgggag gaaaccggcc ggcgtctcga ccccggagac gcgccctccg
     2281 gttcgggcaa cgacccgcgc gccggtcccc tcgatcagcc ggaccccggc gccccggccg
     2341 gccaggacga gggcccggcc gacccgccgc gccctcgcca ccccgtcgcg cgggaaggag
     2401 aaggccgcac ggagctccgg ggcgagagcc ggctcgcggc cgcgcgcctc ctcgggctcg
     2461 aggacctcga acggcatccc ccgcctccgc tgagcgtcga gggcgcggtc gaggagcacc
     2521 tcttccggct cgtcgaacgc caccgtcagg gcaccccgct ggtcgagctc gcccgagacc
     2581 ccggtctcct cctcgaggcc cgacagccag tcggggaaga ggtcgcgcgc gaagagcgcc
     2641 aggcggcccc ccgcctcggg ggtcgcctcc tccacgaacg cctgggccgc aaccggggcg
     2701 ccgggcccga ccgggcggcc gactctcccc ggcgcgagga ggacgacgtc gaggttgcgt
     2761 cgcgcgagct cgagagcgac ggcggccgcc gcgaggccgg ggccgcagac gacgacgtcc
     2821 gcggaagaca gggtccgcgt cactcgaccc gtccctcggc ggcgaggacg gggccccggc
     2881 cggcgagctc gggcggcccc cagcggcgct ccacccgcac gacgacggcc cggccgacgg
     2941 cccggaccgc ggcgtagttc aggaccgccg acgccgcgat cccggccagc gggatcaggt
     3001 gcgcgagcgg acgtccggcg gacctcgccg cgagccgcgt cgcgagcgat tcggccagtc
     3061 cgaccgtgag ccttcgcccg accgacaggc cggcgccggc cgcgagcccc gcgacgacct
     3121 cgagggcgcg ctcctccggg tcgagctcgg ctccgtaaag caggtgcagc ccgacgatca
     3181 tccggctctg gaggacgatc agggtcgtca gctcgggcgc cgtcgccgcg acggccgccc
     3241 accccggagg aacgctggcc gcggcgccga cgagcgcggc cttccgggcc gacgactcca
     3301 ccatcagcgc cgccttcctc cgcgtcgaga ggtcgggatg ccgccgcgag agctcccgca
     3361 ccccgtcgcg gatccgctcc acgtcgaggt ccgtcgaggc gcgccggacg agggcgagga
     3421 aggactcggc aatcttcacg tcggctccgt cgaagtatca tcgcccccgc cccgccgccg
     3481 gaacaaggcg gcgccggcga cggccgccga aggaacaagg aggtcccgtg cacgcgatcc
     3541 gccccgtccg cttcgccctc gccctctcgc tgggtctcgc tctcgcggcg accgcattct
     3601 cgtccgacgc ggccacgccc cagaagaagg gcgcatcgcc ccggaaggcc gcgccgaaga
     3661 aggagaagat ggtgtcgtcc accccgcgcg actacgcgaa gaccctcgcc gtcctgaaga
     3721 cctcccaggg agacgtgacg atccggttct tcttcgacaa ggccccgggc cacgtgaaga
     3781 acttcgtcga cctcgccgcg cgcggcttct acgacggcac gctcttccac cgcgtcatcc
     3841 cgggcttcat ggtccagggc ggagacccgc tcacgaagga cccgagcaag agcgcgctct
     3901 tcggcaccgg cgggaacacc gacaaggcgg gcaagccggt caacgtgaag gccgagttca
     3961 acgacgtgag ccacaagcgc ggcatcctct cgatggcccg cgcgagcgac ccgcactcgg
     4021 cctcgtccca gttcttcctc gtcgtccagg actcgccgtt cctcgaccgc cagtacaccg
     4081 cgttcggcga ggtcgtgaag gggctcgagg tcctcgacaa gctcgtcacc gagtcgaact
     4141 ccgacacgtc cgacccgcgc agcggcggaa agccccgcgc gtaccagaag atcctcaagg
     4201 tcgagctcgt cgccgaggcg gcgcccggaa acgcgtgacg cttcccgccc cggccggcca
     4261 ggaggcgcgc gttccctcgg cgtgctcccg gttcggcctt ccggagtcgg gaccgttcca
     4321 gctcctcgcc ggggacgtcg gacagaggag gtacgtccgc ctgcgcacct gggccgatgc
     4381 gacggtcgtc ctcgtcctct accccgagcc gtcctctccg gcgcaggaga ggtggcgccg
     4441 gatcggggag gcgctcgcac gcgagggcct gcgcgtcccg gctctcctcg acgacgaccc
     4501 cgcctccggg accgcgctcg tcgaggacct cggcgaccgc gacctggcgg cggatctccg
     4561 cgaggcggct cccgacgagc gcgcgcgcct gctcgaggag gccgaggagc tcctcgctcc
     4621 gcttcgcggg atggcgcccg aggccgcgct cctcaacccg ccgttcgacg cggccttcct
     4681 cgcctcggag ctcgggcaca cgcgccggtg ggcgctcgag ggcgacggcg cgcgtccgct
     4741 cgcgccggag cgcgccgccg agtgggagga gctcgcggcc cgcctcgccc gggaagcggc
     4801 cgacgcctcc cgcgtcggcg ggcccgtccc gacccaccgc gacttccacg cgaacaacct
     4861 gatgcgcacc cccgaggggg cgctggcgct gatcgacttc caggacctgc gcctcggcca
     4921 ccccgactac gaccccgtct ccctccgctt cgagcgcgcc ggcgcgctcg tgtcgtcgag
     4981 cggaggcgac cggagcgagg ccgtcctcct ccagcgcgcc tggaaggtcc tcgggacgtt
     5041 cgagaagatg ctcgcgctcg gacgggaggt ctaccggccg caccgggacg cggcgctccg
     5101 cacgatccgc gagcggaccc ggccggacgg gccgttcgcg ccgctcctcg cgttcctgcc
     5161 cggctgagcg ggtatcattc ccgccctcag gaggcccacg tgcccaacct cggactcccc
     5221 gaacttctgg tccttctctt catcgtcgtc ctcctcttcg gcgtcgggaa gctcccgcag
     5281 ctcgggaagg ggatcggaga ggggattcgc aacttcaagc aggccgtcaa ggacggcggc
     5341 caggactccg acaagaagga gcctccgacc ggcaacggct ccgggaacgg cccctccgcc
     5401 ccgaagtgac ctccgtcggc cgccgggcgc cggaatagcg ggccgcgtcc cggcgttttc
     5461 ctcgccgggc aggagcgcgc tcggagccgg aagcgaccgg ccggcgcggc ccgccccacg
     5521 ggagtcgcca gcatgaagat cgccaagcgc gtctttctct ttctcgccgt caacgtcctc
     5581 gtcgtcgcga cgatctcggt cgtcctgagc ctcctcggag tccggccgta cctgaacgcc
     5641 tacggcctcg acctgcaggc cctcgccgtc ttctgcctcg tctggggctt cggcggcgcc
     5701 ttcatctcgc tcgccctctc gcgggtcatg gcgaagtggg ggatgggcgt gaagctcgtc
     5761 gacccgggca cgcccgaccc cggcgcgcag gagctcgtct ccaccgtcca caggctggcg
     5821 caggccgccg gcctccggac catgccgcag gtgggcgtct acgactccga cgaggtcaac
     5881 gccttcgcga ccggaccgac gaagaaccgt gcgctcgtcg ccgtctcgac gggcctcctt
     5941 cgtcggatgc ggcgcgagga ggtcgagggg gtcctcggcc acgagatcgc ccacgtcgcc
     6001 aatggcgaca tggtgacgat gacgctggtc cagggcgtcg tgaacgcgtt cacgatgttc
     6061 ctcgcccgcg tcctcgcgtt cttcgtcgcc cagctcttcc gccgcggcga cgaggaaggg
     6121 ggcggcgtct cccacctcgt ctatttcgtc gcgacgctcg tcttcgacat cgcgttctcg
     6181 atcctcggct ccttcgtcgt cgcctggttc tcgcgcctgc gcgagttccg cgccgacgcc
     6241 ggcggggcgc ggatcgccgg ccgcgaccgg atgatcggcg ccctcgaggc tctccagcgc
     6301 acctacggca tcgtcgaccc gtcggaccgg caggccgccg tccagacgat gaagatctcg
     6361 ggccatccga aggggctcat gcgcttcctc tcgacgcacc cgccgctcga gacgcggatc
     6421 gcccggctca agatgctcgc gtagccgccg tttcgccccg tagtcgcccg agaggccggc
     6481 gcgtcgcgcc ggcctcttcc ctattcggct ccgggcgcgc cgggcagaga cggctcgggg
     6541 gaggcgcgga gagcgcctcc cccgtctgaa cctcgtggtt cgagctcagg cggtcccggt
     6601 catggccggg accttcggct cacgcgtggt cccggcgctt cggggcgggc ttcagacgga
     6661 gcagcgagat cttcgcgcgg agcgtgttcc ggtggatccc gagggacttc gccgcctggc
     6721 ccatgttccc ggaggcccgg gtcacggccg tggcgacgta cttcctctcg aattccttcc
     6781 tcgccgtctc gagggggatc cccttggaaa cgagctcgtc gatgatccgc tggagctgtc
     6841 cgtcgagcat cacggtgacc tcatcctcct cgaagaaagg aagcccgctt cgggcgaaag
     6901 ggacggcaac ttatcacgcg aaggcgcgca gcggaacctc gcgagccggc tcgcgtcaga
     6961 gcggcctcgc gctacttcag ctcgacgaag tctccggcgc gcatcgaatc gaccatcgag
     7021 gtgatcttcg cgacgcaggt cttccccttc gtccagagga cggcggcctc gccgagaagc
     7081 gtccggatcg tgccgctgtc gtttcgggcg cggtagacgg tcaggaagtc tcccggatag
     7141 agaccgtcct cctcgccgag gtcgaggaag accaccgagt cggtcccgac cggcgtcgcg
     7201 cggtccttca cctcgacgat gcggcccgtc accttcccgg acggcgggtc gcactgcgtg
     7261 aggggcgcgc tcgcgcgaac gagcgggatc gggatcggct cgaacgggat caggaggtcg
     7321 ccgaggtagg tcggctcgca cgactccgtg atctcgagga tcgccgaatc gtcctggacg
     7381 cagacgaccc gcacccgcgc cggcgtctcg tagaagcgcc cgatcgtcgc ggtgtacgag
     7441 ccccaccggt agacctcgtg cgccggtcgg atcacctgga actcctgccc cgccatgagg
     7501 ccggcgcgag cgccgacgtc ggagtagatg atgtcgccga cgccgaacat gtgccggctg
     7561 tcctcgtact ccgcggcgac gatccgaccc gtcaccggct cttcgtcgac cccgatccag
     7621 ccggcgcagt agacgtcgtc ttcccatccg accgggaacg gcgcggacgt ggaggtcctc
     7681 tccgtggggg cctgctcgat caccgcgggc gccgcaggtg cttcgacggc ctcctgcggc
     7741 acggggaccg gcgcggcctc ccgcaggccc cccggtccgg cggctgcggc ggagagagaa
     7801 atgacggaac aggcgaggac gaccatcgcg ggaacgaacg cgcgcttcac ggctccgacc
     7861 tcctcgagga cgccgcggtg ccaccgcgcc cgacggcctc ggctcgattc ttccggacta
     7921 tcgatgctaa gttaccgcaa cgccgacgtt tctcaaagcc ccgtcgcggc cgtggcggcg
     7981 acgggggtct ccgggaggcg ggggaccttc cggaggaacc ggctctcggg atggtcccgg
     8041 cggagccggt cgaacagcgc ccgggcgtct tcggtccggc cgagtcgacg ctcggtctgc
     8101 ccggcccagt agagcgcctt gtccatcccc gcgtactcgg ggaaggccgc gaagagccct
     8161 tcgaagcggc tccgggccgc ctgccaggcc ccccgccggt agtagaagcg ggcgacgagg
     8221 tactcgtgct cggccaggag gtcgagaatc gccttgtagc gcgctcgcgc ctcgaacgcg
     8281 tagggcgagc tcgggtagtt cacgacgagc tcgctgtagc tctgggccgc cagccgcgtg
     8341 ttcgcctggt cccggtcggg cttctcggcc tggcggtcgg agacctgcgc gagccggaag
     8401 agcgcgtagt ccgacctcgg atgggacggg tagcggctcc ggaagtcctt gaagcgggcc
     8461 tgcgcctcgg cgtaccccgt ggcggacttc tcctccagga aggagtccgc gaggcggagc
     8521 gccgcctgct tgccgagcgg gtcgttcgcg tagttctcgg ccacgaaccg caggtactgc
     8581 cgccccatct cgaacttcct cttcgtcacg agggcgtccc cctgcgcgta ggcgtcctcc
     8641 ttcgacatcg tcaggagctc ccgcgtgacg cgggacgggt cgctcgagga gcaggcgccg
     8701 gccccgagca cggcgaggcc gaggaggacg gacgcggcgg tcatgcggtg gatgcgatgg
     8761 gccattctct tctccggatt cagcccggct gcggctcggg cgaggcgacg acgaaggaca
     8821 gggtgtcgcg aaggccgacg ggggcgccgg cgagcgggta ctgccgaagg atcgtcccgg
     8881 cggcggcccc ttcgtagggc tgggccttga cgcccccgag gcggaagccg cgcgcctcga
     8941 aggcgaggcg cacccgctcg aagtcgcggc cgatcaggtc gggcatgacg tagaggacgt
     9001 cgggagcacc ccggttcacg aggacgtcca cgggcgtctc cggctccgtg ggcgccccgg
     9061 ggacgacccc ctgcgcgacg acgccctggg gaccggggag ccgcacggcc gagaccgccc
     9121 ccgcgacgag accttcgcgc gtgagcgcga gggcggccgt tcgtgccgtc aggccggcga
     9181 ggtcgggagt ccggatgatc cgcgggccga gggagaggaa gacccgaagg tcctgccccg
     9241 ccttgacggc catcccggcc gccgggctct gcccccggac cctgtgggcc gggatctcct
     9301 cgtcgaaccc ctcctgcgag ccgtcgacga cgacgcggag cccccgctcg gccgcgaggg
     9361 ccgtcgcctc gtcgaccgac cttcccgtca ggtcggggac ctcctccgcc ttcccgagga
     9421 cgaaccggga gaacgagatc catgcggccg ttccgagaat tccggcgagg aggagggcgt
     9481 agaggatccg tgccagaacg ccgctcattc gcgcggactt tagcaggggg cgtgccttga
     9541 cccgctcctc cggggcgagg agactccgtc tccatggtcg aaacccctcg gacgacccga
     9601 tcccgcggcg cggccgcgac gcccgccctc ccgctcgcgc gggcggacct ccacgttcac
     9661 accaacgcct ccattttcaa gtacttcaag gcggccaaca gccacgactc ctacaacgac
     9721 ccggaagagg
//
LOCUS       DAOTEZ010000134         9713 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4951_length_9713_cov_5.062228, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000134 DAOTEZ010000000
VERSION     DAOTEZ010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9713
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4951_length_9713_cov_5.062228"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>9713)
                     /locus_tag="P5164_08715"
     CDS             complement(<1..>9713)
                     /locus_tag="P5164_08715"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44012.1"
                     /translation="AVLCLQPQRRDYFTRSQPISPVKDLRTAPNPYYVSPSNMTAAGS
                     RVFFVAASPGAGTELWVSDGTTAGTHLVKDIRPGAESSGVFGLTTVGSTVFFQADDGT
                     NGVELWRSDGTEAGTVLVKDAEVGPGGSFPNFLATNGSVLFYSYGGPAQELWRSDGTE
                     AGTYELTDIPPEWHNSQPRNLAWVGGALYFAASDPDIGQELWKSDGTAAGTILVKDLR
                     PGSLGSGVRNLTDAGGVVFFVANDGVSGEELWRTDGTPSGTSLVADVSPGAASSSPQL
                     LTNVGGTLFFTANDGTNGTELWKSDGTPGGTTLVKDLCTGCPSNVGNLVAMGGVLYFT
                     ASDGVNGRELWRSDGTEAGTVRITDLCVGGMDGVTGPLVVLAGKVYFQAGGCTASGNE
                     LWSSDGTTAGTAQVKDVCSGGCSSGPGPIVAATTTLFFGANDGTNGPQLWKSDGSDAG
                     TVRVTDATVLSSFPNVFVDLGGTLVFAAADPTANTRTLWKSDGTAAGTAQLAVGRLYN
                     PRAPVLLGGRAHFPGDAGGGDLELWASDGTEAGTVLVKDIWSGGSGSNPTLLTPAGAT
                     LFFLANDGTHGSELWKSDGTESGTVLVKDVRPGGSGANVQNLVAVGSRVFFSADDGVN
                     GRELWVSDGTEAGTTLVEDLSSGGGGSNPGYLAEMGGVLYFSANGPTGNCLYRSDGTA
                     AGTYEVTTSTCTNPMYQTKVGGTLFFTGWTTTGWELCASDGTSAGTRMVRKIYPGGSS
                     SPSGYVAVGSTAYFRANDGVTGTELWRSDGTEAGTWRLADIRPGSGSSLPRAITPMGG
                     AVYFVANDGVHGEEIWKSTGSNEGTFFAQDLNPGTAHSEAGFLTPFGARLVLSGDDGT
                     IGLEPRIIGTAALSVTKTTSTPAPTQGSSVTWTITVSNAGPLPATGLRVIDPLPIGAI
                     FVSSSPGSPTCTITGGTLTCLLGTLASGASTVVTVTATVAVGFTGDLSNTARAVALQA
                     DAEGGNDFATVVVTVPGPQPVTGLYAQPSSATSIALTWNDPSSTQTGIRIERSTDGST
                     FLPVTTVGPHVTAYADLGLTPGALYFYRVVAVQSGVGEAVPSNVAAAQTYSDVATKVC
                     ATKVGPEHQWSRFPAVAWNGTTWAVAMQVRNSEKDLDEIRFQRLDAATGLPTGAPVNL
                     SDDDMNSSYPTIRWNGTRHAVVWYTGMRTAAGAQASSTAMGLLDGSGALVRVNPRIAD
                     GGAYINDNTETALAWNGSEWGVVLPRIVGTQSDLYFTRVSSAGDAVGASVRLTETPEM
                     EIDVSLAWNGSVWGVSWVRRTAPQRVWFQRLQADGTPLGTPTLVYENVAGTDVLTTSL
                     VWDGTGWALAWDDVGPGPGYSERITLARLNADGSPNGAPVPVSPEYDPDFPPAEQVPN
                     YDSVPQLFAKPGGGYVVFTSATLNTNYFIEIGRYETDSSGNRIGSRTILSANDDHYSV
                     YSRAAFDGTKYLVAYNDGSSSRQEVARFVAGASGPALVAPALLSSGASPGDTWGVASS
                     GSPVALATGKRFTLVWTEPASGTGSRLHATVVDASGAVTANRWPLTTRETRTRPGAVA
                     AGNSFALAWKEPNDDVWFDRYDANGSSLLGEVLVAPMQYGRAAVGVAFSGEAYGVAWI
                     PGANLCFQRVSPSGALLGARTSLALPSTPTTTPLQIAWTGTGWAVVYTRGTRLEYVLL
                     DRSGSVSVGPVAISATSNAKGALSAAWSGSRLGVAWVESTGSPSLLNDVLFTVVAPNG
                     VPDFAPVAAVSSAWNDGNPSLYWAGDRFGLVHSFGEYPVLREVGLFPNGSFASSPRLL
                     NPRGGANAAAWNGATLGVLWPQQSDLYFETTECLADATPPTCPSATAVRTGQEMTISI
                     GPGADAESGVFRNYLYRDGVLLAELFPETTTVHDGGLLPGVAPTYEARAMNGAWLESA
                     GCVAIKPSTDLAATISDAPDPVAQVQNVTYTATATNTGLSPATAVVLTVAVPTQATFQ
                     SSSPGSPTCAHSAGTVTCSLGGLAPGAASSVNVVVRPKAGTAGAIVATASADGAEGDP
                     NAANDSASATTTVLAPVPARDLVVTARTTTTVSLAWTDPQGGETAFRVERSTDGTSFT
                     AINNLGPDTATYVDTGRSPATLYFYRVVAVQNLVGEAPPSNVAAATTLAATAAKVCAT
                     PLGPFAGNASGPTLAWTGTSWAAAWTDRRDGLAEEVWFQRLDAATLAPVGSAVRVSQT
                     GAPSGARALRWNGSRLGLLWNEMMKRPDGTNVYDYRFTAFDAAGNRLQGDVPAFRLEA
                     RATHGPAEVPLLWNGTGWGAFVLENPAFPNDIRYFRLDANGRPVGAPVAVTATSDWEV
                     ALSVAWSGTEYGVAFTRTGLNGGAYQILLQRMAVDGTLLGAPAVVWENTSLGEGEFLS
                     GTTSVAWDGAAWVVAFTDIGPLPDQAEAVWLRRVSAAGVPSGAAVRLSPAFDPDMPPE
                     DQAPVIDQTPTILPKAGGGFVVLTSSWLNTTRYREVCRYEANASLARVGTRIVVSTGD
                     TRNSPSPRAATDGTRALVAWNEGRSGTPEVAAALLDAAGGTLVAPTEITGGHTLGYSG
                     NPVALALGSGFGALYTYQDASLAQTSIGGAFWNGSGSLVATRTPLSARGIRGAPGAAA
                     AGSSFAVAWKDFANNVAFARYDATGAPVGSETVVATGAGGRPYVGIAFGGDSYGLAYW
                     SGANLYLHRVAPDGTTLGVRALVTTTTENGAPGPQIQWVGSGWAIVWLQETHLFYARL
                     DAAGVVVVPPIQVTFTPQTEAAYQLYFTGQHLGLAWHEYAVPDPPGGEVFFTVLGLDG
                     IKAFPPIAIGSSDLDDYYPSISWDVDRFRVVMVPNNPGVLREIPILPNGTVPPGERML
                     WNAGGLNSVAWNGATLGLAWQTGGYIHFQTTACLADASAPPCPAATAVFDGARTTVAW
                     APVTDADSGLWRYDVYRDGLLVAEERSGTTSWIDTAPPPGVAHAYGVRALNKALLESA
                     ACATASPAANLGVTVADAPDPVAPGGAVTYTLTVSNAGPSAASGVSLVDTLPAGVTFS
                     SSSPSSPDCAHAAGVVTCALGTIASGANRVVTIVVTLDSGTSGTLTNTASVTGVEDDP
                     VPANDNASSTTAVTVLAQTLTVTRDGAGTGSVFSNPLGISCGATCVASFAPGAVVALT
                     PVPNAGSTFTGWSGACTGAGACSVTMSAARAVNATFALAPVLPQIIAFTATPAVLVAA
                     GSSTLAWSTAGADSVTIRIGDTVMPFGPAGATAFAVGTTSTYLLTATNAAGSVTATAT
                     VNV"
BASE COUNT         1358 a         3275 c         3686 g         1394 t
ORIGIN      
        1 gacgttgacg gtggcggtgg cggtgacgct gccggcggcg ttggtggcgg tgaggaggta
       61 tgtgctcgtc gtgccgacgg cgaaggcggt cgcgcccgcc ggaccgaaag gcatgacggt
      121 gtcgccgatc ctgatggtca cagaatcggc cccggccgtg ctccaggcga gggttgagga
      181 ccctgcagcg acgaggaccg ccggtgtggc cgtgaaggcg atgatctgcg ggaggacggg
      241 ggcgagggcg aaggtcgcgt tgacggctcg ggccgcggac atcgtgacgc tgcacgcgcc
      301 ggcgcccgtg caggctccgc tccagccggt gaaggtggag cccgcattcg gaacgggcgt
      361 gagcgcgacg acggcgccgg gggcgaagga ggcgacgcac gtcgctccgc agctgatgcc
      421 gagggggttg gagaagacgg agccggtgcc ggcgccgtcg cgggtgacgg tgagggtctg
      481 ggcgagaacg gtgacagcgg tggtcgagct cgcgttgtcg ttcgctggca ccgggtcgtc
      541 ctcgacaccc gtgaccgaag cggtgttcgt gagcgttccg gacgtacccg agtcgagagt
      601 gacgacgatc gtcacgaccc ggttcgctcc ggacgcgatc gtgccgagcg cgcaggtgac
      661 gacgcccgcg gcatgggcgc agtcgggcga cgacgggctc gacgaggaga aggtgacgcc
      721 cgccggaagc gtgtcgacga gggagacgcc ggacgcggcc gacgggccgg cgttcgagac
      781 ggtcagcgtg taggtgaccg cgccgccggg cgcgaccggg tcgggcgcgt cggcgacggt
      841 cacgccgagg ttggccgcgg ggctcgcggt cgcgcaggcg gcagattcca agagcgcctt
      901 gttcagcgcc cgaaccccgt aggcatgcgc gacgcccggg ggcggcgcgg tgtcgatcca
      961 ggaggtcgtc ccggagcgct cctcggcgac gaggaggccg tcgcggtaga cgtcgtagcg
     1021 ccagaggccg gagtcggcat cggtgacggg tgcccacgcg acagtcgtcc tcgcgccgtc
     1081 gaaaacggcc gtggccgccg gacagggggg agcggaggcg tcggcgaggc acgcggtcgt
     1141 ctggaagtgg atgtagccgc ccgtctgcca ggcgaggccc agcgtcgcgc cgttccaggc
     1201 gacgctgttg agccctccgg cgttccagag catccgctcg ccgggaggga cggtgccgtt
     1261 cgggaggatc gggatctccc tcaggactcc cgggttgttg gggaccatca cgacgcggaa
     1321 ccggtcgacg tcccaggaga tcgacgggta gtagtcgtcg aggtcgctcg atccgatcgc
     1381 gatcggcggg aacgccttga ttccgtcgag accgagcacc gtgaagaaga cctcgccgcc
     1441 gggcgggtcg gggacggcgt attcgtgcca ggcgaggccg aggtgctggc cggtgaagta
     1501 gagctggtag gccgcctcgg tctggggtgt gaacgtgacc tggatcggcg gcacgacgac
     1561 gacccctgcc gcatcgagcc gcgcgtagaa gaggtgagtc tcctggagcc agacgatcgc
     1621 ccagccggag ccgacccact ggatctgcgg tcccggcgcg ccgttctccg tcgtcgtcgt
     1681 gacgagggcg cggacgccga gggtcgtgcc gtcgggcgcg accctgtgga ggtagaggtt
     1741 tgcgccgctc cagtacgcga ggccgtagga gtcgccgccg aaggcgatgc cgacgtaagg
     1801 gcggccgccg gcgccggtcg cgacgaccgt ctcggatccg acgggcgcgc ccgtcgcgtc
     1861 gtagcgggcg aacgcgacgt tgttcgcgaa gtccttccag gcgactgcga aggagctccc
     1921 ggcggcggcc gctcccggcg ccccgcggat gccgcgcgcg gagagaggcg tccgcgtcgc
     1981 gacgaggctt cccgagccgt tccagaacgc cccgccgatc gacgtctggg cgagggacgc
     2041 gtcctggtag gtgtagagcg ccccgaaccc gctcccgagc gcgagcgcga ccgggttgcc
     2101 cgagtatccg agcgtgtgcc cgcccgtgat ctccgtcggg gcgacgagcg tcccgccggc
     2161 ggcgtcgaga agagccgcgg cgacctccgg ggtgcccgac cgcccctcgt tccaggcgac
     2221 gagcgcccgc gtgccgtcgg tcgcggcgcg gggcgacggc gagttcctcg tgtcgcccgt
     2281 ggagacgacg atccgggtgc cgacgcgcgc cagcgaggca ttcgcctcgt accggcagac
     2341 ctcgcggtac cgggtcgtgt tcagccagct cgacgtgagg acgacgaacc cgccgccggc
     2401 cttcgggaga atggtcggcg tctggtcgat gacgggggcc tgatcctcgg gcggcatgtc
     2461 cgggtcgaag gccgggctga ggcgcacggc cgccccggac ggcacgcccg cggcgctcac
     2521 gcggcgcagc cagacggcct cggcctggtc ggggaggggg cctatgtcgg tgaacgcgac
     2581 gacccacgcg gcgccgtccc acgccacgga ggtcgttccg gagaggaact cgccctcgcc
     2641 gagcgacgtg ttctcccaga cgacggccgg cgcgccgagg agcgtgccgt cgaccgccat
     2701 ccgctggagg aggatctggt aggcgccgcc attgaggccg gtccgggtaa aggcgacgcc
     2761 gtactcggtc ccgctccagg cgacggagag cgcgacctcc cagtcggagg tggcggtgac
     2821 cgcaaccggg gcgccgacgg ggcggccgtt cgcgtccagg cggaagtagc ggatgtcgtt
     2881 cgggaacgcc ggattctcga ggacgaacgc tccccagccg gtgccgttcc agaggagcgg
     2941 gacctcggcc ggcccatggg tcgcgcgagc ctcgaggcgg aaggccggca cgtcgccctg
     3001 gaggcgattg cccgccgcgt cgaacgcggt gaaacggtag tcgtagacgt tcgtgccgtc
     3061 gggccgtttc atcatctcgt tccacaggag cccgagcctc gagccgttcc agcgaagggc
     3121 ccgcgcgccc gacggggcgc ccgtctgcga gacgcgcacg gcgcttccga ccggcgcgag
     3181 cgtggccgcg tcgaggcgct ggaaccagac ctcctccgcg aggccgtccc ggcgatcggt
     3241 ccaggcggcg gcccacgatg tgccggtcca ggcgagcgta ggtccggacg cgtttccggc
     3301 gaagggcccg agcggagtcg cgcagacctt tgcggccgtc gcggcgagcg tcgtcgccgc
     3361 cgcgacgttc gacggcgggg cctcgccgac gaggttctgg acggcgacga cgcggtagaa
     3421 gtagagcgtc gccggcgacc gtcccgtgtc gacgtaggtc gcggtgtccg gccccaggtt
     3481 gttgatggcc gtgaacgaag tcccgtccgt cgaacgctcg acccggaaag ccgtctcgcc
     3541 tccctgcggg tcggtccacg ccagcgacac ggtcgtcgtc gtgcgcgccg tgacgacgag
     3601 gtcgcgcgcc gggaccggcg cgaggacggt cgtcgtcgcg ctcgcggagt cgttcgcggc
     3661 gttcgggtcc ccctcggccc cgtcggcgct cgcggtggcg acgatcgccc ccgccgtccc
     3721 ggccttcgga cggacgacga cgttgacgga cgacgcggcg cccggtgcga ggccgccgag
     3781 agagcacgtc acggttccgg cggagtgggc gcacgtcggg gagcccggcg aagagctctg
     3841 gaacgtcgcc tgcgtcggaa ccgcgacggt gaggacgacc gccgttgccg gcgaaaggcc
     3901 cgtgttcgtc gcggtggcgg tgtaggtcac gttctggacc tgcgcgaccg ggtcgggcgc
     3961 atcgctgatc gtggctgcga ggtcggtcga cggcttgatc gcgacgcagc cggccgactc
     4021 gagccaggcg ccgttcatgg cccgcgcctc gtacgtcggc gcgacgcccg gcaggaggcc
     4081 gccgtcgtgg acggtcgtcg tctccgggaa gagctcggcg aggaggacgc cgtcgcggta
     4141 gaggtaattc cggaagacgc ccgattcggc gtcggcgccc gggccgatcg agatcgtcat
     4201 ctcctgaccg gtccgaacgg ccgtggccga cgggcaggtc ggaggcgtgg cgtccgcgag
     4261 gcactcggtc gtctcgaagt agaggtcgga ctgctgcggc cagaggacac cgagcgtcgc
     4321 cccgttccag gccgcggcgt tggctccgcc gcggggattg aggaggcgcg gggacgacgc
     4381 gaagctgccg ttcggaaaga ggccgacctc gcgcaggacg ggatactcgc cgaagctgtg
     4441 gacgaggccg aatcggtcgc cggcccagta gagcgagggg ttcccgtcgt tccaggcgct
     4501 gctgacggcc gcgaccggag cgaagtcggg gactccgttc ggcgcgacga ccgtgaagag
     4561 gacgtcgttc aggagcgaag gtgaacccgt cgactccacc caggccacgc cgaggcgcga
     4621 gccgctccac gcggccgaca aggcgccctt cgcgttcgag gtcgcgctga tcgcgacggg
     4681 gccgacggag acggacccgg aacggtcgag gaggacgtac tcgagccggg tgccgcgcgt
     4741 gtagacgacg gcccagcccg ttcccgtcca ggcgatctgg agcggcgtgg tggtcggcgt
     4801 gctcgggagg gcgaggctgg tcctcgcgcc caggagcgcg cccgagggcg agacgcgttg
     4861 gaagcagaga ttggcgccgg ggatccacgc gacgccgtag gcctctccgc tgaacgcgac
     4921 gcctacggcg gcccggccgt actgcatcgg ggcgacgagg acctcgccga ggagcgagct
     4981 cccgttcgcg tcgtaccggt cgaaccagac gtcgtcgttc ggctccttcc acgccagggc
     5041 gaagctgttg ccggccgcga cggcgcccgg ccgggtccgg gtttcccggg tcgtgagcgg
     5101 ccagcgattc gccgtcaccg cgcccgaagc gtcgacgacg gtcgcgtgga gccggcttcc
     5161 cgttcccgac gccggctcgg tccagaccag ggtgaagcgc ttgcccgtgg cgagagcgac
     5221 cggactgccg ctggaggcga caccccaggt gtcgcccggg ctcgctccgc tcgagaggag
     5281 cgcgggggcg acgagcgcgg ggccggaggc ccccgcaacg aaccgggcga cctcctggcg
     5341 gctcgaggag ccgtcgttgt aggcgacgag gtacttcgtc ccgtcgaagg cggcccggga
     5401 gtagacggag tagtggtcgt cgttcgcgct caggatcgtg cgcgagccga tccggttccc
     5461 ggaggagtcc gtctcatacc gcccgatctc gatgaagtag ttcgtgttga gcgtcgcgga
     5521 ggtgaagacg acgtagccgc ctcccggctt cgcgaagagc tgggggaccg aatcgtagtt
     5581 cggcacctgc tccgccggcg ggaaatcggg gtcgtactcg gggctgacgg ggacgggcgc
     5641 gccgttcggc gagccgtcgg cgttcagccg cgcgagcgta atccgttcgg agtagcccgg
     5701 acccggcccg acgtcgtccc aggcgagcgc ccaccccgtg ccgtcccaca cgaggctcgt
     5761 cgtaaggacg tccgtgccgg cgacgttctc gtagacgagg gtcggagtgc cgagaggggt
     5821 tccgtccgcc tggaggcgct ggaaccagac ccgctgcggc gctgtccgcc tcacccacga
     5881 gacgccccag acgctgccgt tccaggcgag ggagacgtcg atctccatct ccggcgtctc
     5941 cgtcaggcgg acggaggcgc cgacggcgtc gcccgcggac gagacgcgcg tgaagtagag
     6001 gtcgctctgc gtgccgacga tcctcgggag gacgactccc cactccgacc cgttccacgc
     6061 gagagcggtc tcggtgttgt cgttgatgta ggccccgccg tcggcgatcc gcgggttgac
     6121 tcggacgagc gcgccgctcc cgtcgagaag ccccatcgcg gtcgacgagg cctgggcgcc
     6181 cgcggcggtc cgcatccccg tgtaccagac gaccgcgtgc ctcgtcccgt tccagcggat
     6241 cgtcgggtag gaggagttca tgtcgtcgtc gctcaggttc accggcgcac ccgtcggaag
     6301 gcccgtcgcg gcgtcgaggc gctggaagcg aatctcgtcg aggtccttct cggaattgcg
     6361 gacctgcatc gcgacggccc aggtcgtgcc gttccaggcc acggccggga agcggctcca
     6421 ctggtgctcc gggccgacct tcgtcgcgca gaccttcgtc gcgacgtcgg agtacgtctg
     6481 tgctgctgcg acgttcgacg ggaccgcctc gccgacgccg gactgaaccg cgacgacgcg
     6541 gtagaagtag agggcgccag gagtcaggcc gaggtcggcg taggcggtga cgtggggccc
     6601 gacggtcgtt acgggaagga acgtcgaccc gtccgtcgag cgctcgatcc ggattcccgt
     6661 ctgagtcgac gacgggtcgt tccacgtgag cgcgatcgac gtcgccgagg aaggctgggc
     6721 gtagaggccc gtcaccggtt gcggtccggg aacggtcacg acgacggtgg cgaagtcgtt
     6781 cccgccctcg gcgtcggcct ggagggcgac ggctcgggcg gtgttcgaca ggtcgccggt
     6841 gaagccgacg gccacggtcg ccgtgaccgt gacgacggtg ctcgctccgg acgcgagcgt
     6901 gccgaggagg cacgtgagcg tcccgccggt gatcgtgcac gtcggagagc ccggcgagga
     6961 ggagacgaag atcgcgccaa tcgggagcgg gtcgatcacc ctcaggccgg tggccggaag
     7021 cgggccggcg ttcgagacgg tgatggtcca ggtgacggag cttccctgcg tgggggcggg
     7081 cgtggaggtc gtcttcgtga cggagagggc cgcggtcccg atgatgcgag gctcgaggcc
     7141 gatcgtcccg tcgtcacccg agaggacgag ccgcgcgccg aagggcgtga ggaatcccgc
     7201 ttcggagtgc gccgtgccgg ggttcaggtc ctgggcgaag aaggtcccct cgttcgaccc
     7261 ggtggacttc cagatctcct cgccgtggac gccgtcgttc gcgacgaagt agaccgcgcc
     7321 tcccatgggc gtgatcgctc gcgggagcga ggagccgctg cccggccgga tgtcggccag
     7381 gcgccacgtg ccggcctcgg tgccgtcgct tcgccagagc tccgtgcccg tgacgccgtc
     7441 gttcgcccgg aagtaggcgg tgcttccgac ggcgacgtag cccgacgggc tcgacgaccc
     7501 gccgggatag atcttccgga ccattcgcgt gccggccgag gtgccgtcgc tcgcgcagag
     7561 ctcccatccg gtggtcgtcc agccggtgaa gaagagcgtc ccgccgacct tcgtctggta
     7621 catcgggttc gtacaagtgc tcgtcgtgac ctcgtacgtg ccggcggcgg tcccgtccga
     7681 gcggtagagg cagttgccgg tcgggccgtt cgccgagaag tagagcaccc cgcccatctc
     7741 ggcgaggtac ccgggattcg atcctccgcc ccccgatgag aggtcttcga cgagcgtcgt
     7801 cccggcctcc gtcccgtcgc tcacccagag ctcgcgcccg ttcacgccgt cgtcggccga
     7861 gaagaagacg cgagagccga cggccacgag gttctgcacg ttcgccccgc tcccgcccgg
     7921 ccggacgtcc ttcacgagga ccgttcccga ctcggtcccg tcgctcttcc agagctcgct
     7981 tccgtgcgtc ccgtcgttcg cgaggaagaa gagcgtcgct cccgcggggg tgaggagcgt
     8041 cgggttcgac ccgcttccgc cggaccagat gtccttcacg aggaccgtcc ccgcctcggt
     8101 gccgtcgctg gcccaaagct ccaggtcgcc cccgcccgcg tcgcccggga agtgggcgcg
     8161 gccgccgagg aggaccggcg cgcgcgggtt gtagaggcgc cctaccgcga gctgagccgt
     8221 gccggccgcc gtgccgtcgc tcttccagag ggtccgggtg ttcgccgtcg ggtcggccgc
     8281 ggcgaagacg agcgtcccgc cgaggtcgac gaaaacgttc gggaacgacg agaggaccgt
     8341 cgcgtcggtc acgcggacgg tccccgcgtc gctcccgtcg ctcttccaga gctgcggacc
     8401 gttcgtgccg tcgttcgctc cgaagaagag ggtggtggtt gcggcgacga tcgggcccgg
     8461 cccggacgag cagcctccgg agcagacgtc cttcacctgg gccgtccccg ccgtcgtccc
     8521 gtcggacgac cagagctcgt tgccgctggc ggtgcacccg cccgcctgga agtagacctt
     8581 gcccgcgagg acgacgagag gtccggtgac tccgtccatg cctcccacgc agaggtccgt
     8641 gatgcggacg gttcccgcct ccgttccgtc gctccgccag agctcgcggc cgttgactcc
     8701 gtcggaggcc gtgaagtaca ggacgccgcc catcgcgacg aggttgccca cgttcgacgg
     8761 gcatccggtg cagaggtcct ttacgagcgt cgtccccccc ggggtcccgt cgctcttcca
     8821 gagctccgtc ccgttcgtgc cgtcgttcgc ggtgaagaag agcgtcccgc cgacgttggt
     8881 caggagctga gggctcgagc tcgcggcgcc cgggctgacg tcggcgacga gagacgttcc
     8941 cgacggtgtg ccgtcggtcc tccagagctc ctcgccgctc acgccgtcgt tcgcgacgaa
     9001 gaagacgacg ccgcccgcgt cggtgaggtt ccgtacgccc gatcccaggc tcccgggccg
     9061 gaggtccttg acgagaatgg tcccggccgc cgtcccgtcg ctcttccaga gctcctggcc
     9121 gatgtcgggg tcggaggccg cgaagtagag cgctccgccg acccacgcga gattccgggg
     9181 ctgggagttg tgccactcgg gcggaatatc cgtcagctcg tacgttccgg cctccgtgcc
     9241 gtcgctgcgc cagagctcct gcgcggggcc gccgtacgaa tagaagagga cgctcccgtt
     9301 cgtcgcgagg aagttcggga aggagccgcc cggtccgacc tcggcgtcct tcaccaggac
     9361 cgtcccggcc tcggttccgt cgctcctcca cagctcgacg ccgttcgttc cgtcgtcggc
     9421 ctgaaagaag acggtgctgc cgacggtcgt gaggccgaag acgcctgagc tttccgcgcc
     9481 gggccggatg tccttcacga ggtgcgtccc cgccgtcgtc ccgtcgctca cccagagctc
     9541 ggtccccgcg ccgggcgagg cagcgacgaa gaagacgcgg ctcccggcag cggtcatgtt
     9601 cgacggcgaa acgtagtacg ggttcggcgc cgtgcgcagg tccttgacgg gcgagatcgg
     9661 ctgagagcgt gtgaagtaat cgcgccgctg gggttgcaaa cagagaacgg cgt
//
LOCUS       DAOTEZ010000135         9643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_4989_length_9643_cov_6.260743, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000135 DAOTEZ010000000
VERSION     DAOTEZ010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9643
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4989_length_9643_cov_6.260743"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /gene="dctP"
                     /locus_tag="P5164_08720"
     CDS             <1..682
                     /gene="dctP"
                     /locus_tag="P5164_08720"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRY44013.1"
                     /translation="EVDHVLAVYGPKLESSIAAKGFVVLNWADAGWVRFFTKTPVRTP
                     ADLKPLKLFAWGNDMDALEIWKGAGFNPVPLPSTEISTALQTGLVSALPTTPTAAVLL
                     QWYQHAKNLTDVRWALLLGATVVNKATWDKVSPEDQKALRAAAAEAGARLRAESRAAE
                     ARDIAAMQKRGLNVIAIDAKTEALWRTAAEAAYPKVRGKIVPEAAFDEARKILADFRA
                     KQAAAPKK"
     gene            679..2496
                     /locus_tag="P5164_08725"
     CDS             679..2496
                     /locus_tag="P5164_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRY44014.1"
                     /translation="MKAGALLGKAEQALLVAALAVATVLPLVDAFGRPLGGFHVPGSG
                     SILQLVTLWIAFVGGLLATREGTHLTLSTAELLGSGRARRLARNFAFSVAAAVCAVLS
                     WASVGLVRADREQAKVLSFGLPEWIAELVMPAALLLMAVRFAWKASDGAPGRAAALAV
                     LASPFLLSLVPEANAPKLVLPLAGVILLAALLGAPVFVAMGGLACLLFWKDAVPVAAV
                     SAEVYRLVASPTLPAIPLLTAAGYILAEGGASARLVRFFRALFGWMPGGIAVMVAAVC
                     ALFTTFTGGSGVTIIALGGLVYPILRDDGYPEGFSLGLVTAAGSLGLLFPPSLPVILY
                     SVVASVPADSLYLAGLVPGLLLVVLTAAWGIRQGLKLEAEGRSRPRFALRELGASFWE
                     AKWELALPVLVVVLFASGFASMVEASAAACAYAAVVECLVTRDIHPFRQLPGVLVKAG
                     ALMGAVLILLSIAMGLTSWLVDAMVPTALLEWVKGHVSSPLVFLLVLNGILLVLGSVL
                     EIYSAIVILAPLVAPMGAAFGIDPIHLGVIFLANLELGFLFPPVGLNLFLSSSRFGIP
                     LPKLYRHAVPYLLILGAGVLLITYVPAMSTGILSLLGKR"
     gene            complement(2576..3910)
                     /locus_tag="P5164_08730"
     CDS             complement(2576..3910)
                     /locus_tag="P5164_08730"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRY44015.1"
                     /translation="MKNVLARRIAAALVAAAFGVACVTNPVTGKSQLSLLGEEEEKAL
                     GAQAYGPLIQESYGPVNDPGLQKFLDGVGQGLAKVSHRPALEYEFTVVNANYDNAFAL
                     PGGKICITRGLLSRMTTEDQLAGVVGHEVGHVTARHGAQQYTKQMLIGGLLGIGGMIL
                     EAEEVRGAPLIMAAAGIGGQLVLLRYSRDHERQSDELGMEYMARAGYNPKGFVESMEI
                     LKAGHDKEPSKLEAMFQSHPLTSERIETARRRSAEKYPEQLGRPARAEAFNASTRALK
                     AEAPAFKLMDEGEKLLAKKDARGAAARFDEASRLAPRQAILPALKAIALVEANELRSA
                     RESAREAARRDPALFHGRLAGGLAAYRLSDWRDAISELETAEKSVGAQLVTTYYLGRS
                     LEALGDRGQAAEKYKLILQSGATGEQADYAKRRLLEWGVISQTPQQQQPYPR"
     gene            complement(4029..6194)
                     /gene="pta"
                     /locus_tag="P5164_08735"
     CDS             complement(4029..6194)
                     /gene="pta"
                     /locus_tag="P5164_08735"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HRY44016.1"
                     /translation="MRTTILVAPAGRGVGLTTACLGIVRALDREGIRVAFAKPVANRE
                     PDHTIPLLSLGAHFAIPPAVPRAEAEGLLADGDDQTLMERVVAIANEAGEDVDVLIVQ
                     GMHPEMGMVHSARVNALMLKALDAELVLVAAPSDQTAAEVAETVAIAARGYGAFSEGR
                     TVGAILNRVCDRKGAPADVDADSFGPSAGGCGACAPQGLSDECLKEHQTALEAEGIRP
                     IAIVPCNAGLMSPRVKDIGDALRAQFLYTGDAATRRVRDVAICAMTVPNAMKAMKPGT
                     LVLTPGDRSDILLAAAMTVQSGTPLAGVVLTGGLGTDRGVLDLCRKVFDGGLPLLQVK
                     ASSYRAAAQVASMNPTIPIDDADRVERTMSFVADHVDLGWARGFAKPARVPRLSPPAF
                     RHRLVETAKADVKRIVLPEGAEPRTVAAAAIVAARGIAKCVLLGNPDEVRDVAGRQGV
                     TLPDSVEIVNPASVAHRYIAPLVERRKAKGMTPERAELELQDAIMVGTMMMALEEADG
                     LVSGAVHTTAHTIRPALQIIKTVPGCNLVSSIFFMCLPDQVLVFGDCAVNPNPTASEL
                     ADIAIQSADSALAFGVPARVAMLSYSTGTSGTGEDVEKVKEATRIAKELRPDLALDGP
                     LQYDAAVMPDVARLKAPKSDVAGKATVLIFPDLNTGNVTYKAVQRSANVVSIGPMLQG
                     LAKPVNDLSRGCLVEDIVFTINLTAIQAQQAARARASKG"
     gene            complement(6217..7401)
                     /locus_tag="P5164_08740"
     CDS             complement(6217..7401)
                     /locus_tag="P5164_08740"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HRY44017.1"
                     /translation="MNVLVLNCGSSSLKFAVIDPDTSAEHVSGIAQRLGSPEASLDVK
                     RNGTKSSRPIPGALHDEALHGVVDALKELGLADGLLAVGHRVVHGGSKFAGSMAITPE
                     VVAKMEECIPLGPLHNPPNLVGIRIAQNLFPDLLQVGVFDTAFHQTMPPVAFTYPVPY
                     EWLEQHGVRRYGFHGTSHRFVAREALRLAGLPADDHTIVTAHLGNGCSAAAIRNGKSV
                     DTTMGLTPLEGLMMGTRCGSIDPAIVGHVADATGKSAKEVLDALNKKSGLLGVSGLSN
                     DMRTVEEAASNGNDRAKLALDIFCYVLAKAIAALVVPLGRLDALVFTGGIGENSTTVR
                     EMTIARLGFLGIALDEAANAKHGRTQNGRITIGTKPQALVVPTNEELMIAEDAAQIAR
                     GA"
     gene            7704..8513
                     /locus_tag="P5164_08745"
     CDS             7704..8513
                     /locus_tag="P5164_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44018.1"
                     /translation="MRTRTAAALALAFAASLPAAAATRGGYELSVLVDGSERSELRKG
                     GAVYVEALKGREYALRVTNPLGVRVGVALAVDGLNTIDATHRDAASAVKWVLEPYQTI
                     VLEGWQVSSSDARRFVFTGERDSYGAALGKTEDLGVIEAVFFPEKVRRPAPVSPMACD
                     GRDSSRQSAGAPPAPRSKGEGAGGRAEAEKSAASALSDEYAATGMGDRTRHEVARVDL
                     DLERTPAARIRIRYEFRPQLVRLGLLPPEGDRLARRERARGFDRYCPEPDR"
     gene            8683..9147
                     /locus_tag="P5164_08750"
     CDS             8683..9147
                     /locus_tag="P5164_08750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRY44019.1"
                     /translation="MKVSQRGLYALKALLHLAERWDGGTVSTREIAEREEIPERFLES
                     ILVLLKNARIVTSRRGREGGYRLRRPPAEIVVGDVVRLVDGPLAPFGDAVELARLVES
                     EPRHAGLFELFLDLRNAASAILDHTSLADLLERDRRILVARAERAPGATVKQ"
     gene            9207..>9643
                     /locus_tag="P5164_08755"
     CDS             9207..>9643
                     /locus_tag="P5164_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRY44020.1"
                     /translation="MLSIHARGGAAPPARVELLNVSYDPTRELYREVNAAFSREYLAK
                     TGTAVTVKMSHGGSGKQARSVIDGLKADVVTLALAYDVDALADRGLVRPGWQARLPHN
                     SSPYTSTIVFLVRSGNPKGIRDWTDLARPGVSVICPNPKTSGAA"
BASE COUNT         1208 a         3566 c         3357 g         1512 t
ORIGIN      
        1 cgaggtggac cacgtcctcg ccgtctacgg accgaagctc gagtcgtcga tcgccgcgaa
       61 ggggttcgtc gtcctgaact gggccgacgc gggctgggtc cgcttcttca cgaagacgcc
      121 ggtccgcacg cccgcggacc tcaagcctct caagctcttc gcctggggaa acgacatgga
      181 cgccctcgag atctggaagg gagccggctt caacccggtc ccgctcccct cgaccgagat
      241 ctcgacggcg ctccagaccg gcctcgtctc cgccctcccg acgacgccga cggcggccgt
      301 cctcctccag tggtaccagc acgcgaagaa cctgaccgac gtgaggtggg cgctcctcct
      361 cggggcgacc gtggtcaaca aggcgacgtg ggacaaggtc tctccggagg accagaaggc
      421 gctccgcgca gccgccgccg aggcgggagc gcgcctccgg gccgagagcc gcgcggccga
      481 ggcgcgcgac atcgcggcga tgcagaagcg cggcctgaac gtgatcgcga tcgacgcgaa
      541 gacggaggcg ctctggcgga ccgcggccga agcggcctac ccgaaggtcc gcgggaagat
      601 cgtccccgag gcggccttcg acgaggcgcg gaagatcctg gccgacttcc gggcgaagca
      661 ggccgcggcg ccgaagaagt gaaggccggc gcgctcctcg ggaaggcgga gcaggcgctt
      721 ctcgtcgccg cgctcgcggt cgccaccgtt ctcccgctcg tcgacgcgtt cggccgcccg
      781 ctcggcggct tccacgtccc gggctccggg tcgatcctcc agctcgtcac gctctggatc
      841 gccttcgtcg gcggcctcct cgcgacgcgc gaagggacgc acctgacgct ctcgacggcc
      901 gagctgctcg ggagcggccg ggcccggcgg ctcgcgcgga acttcgcctt ctcggtcgcc
      961 gcggccgtct gcgccgtcct ctcctgggcg agcgtcggcc tcgtgcgggc cgaccgcgaa
     1021 caggcgaagg tcctctcgtt cggcctcccc gagtggatcg ccgagctcgt catgccggcg
     1081 gccctcctcc tgatggccgt ccgcttcgcc tggaaggcct cggacggggc tccgggccgc
     1141 gccgcggccc tcgcggtgct cgcctccccg ttccttctct ccctcgtccc cgaggcgaac
     1201 gcgccgaagc tcgtcctccc gctcgcgggg gtcatcctcc tcgcggcgct cctcggagcc
     1261 cccgtcttcg tcgcgatggg agggctcgcc tgtctcctct tctggaagga cgccgtcccc
     1321 gtcgccgccg tgtcggccga ggtctaccgg ctcgtcgcct cgccgacgct cccggcgatc
     1381 cctctcctga cggccgcggg ctacattctg gcggaggggg gcgcgtcggc gcgcctcgtc
     1441 cgcttcttcc gcgcgctctt cggctggatg ccgggcggca tcgccgtgat ggtcgcggcg
     1501 gtctgcgccc tcttcacgac gttcacaggc ggctcgggcg tcacgatcat cgccctcggc
     1561 ggcctcgtct acccgatcct ccgcgacgac ggctacccgg agggcttctc gctcgggctc
     1621 gtgacggccg cggggagcct cggcctcctc ttcccgccgt cgctccccgt catcctctac
     1681 agcgtcgtcg cctcggtccc ggccgactcg ctctacctcg ccggcctcgt cccgggactc
     1741 ctcctcgtcg tcctgacggc ggcctggggg atccggcagg ggctgaagct cgaagcggag
     1801 ggccggtcga ggccgaggtt cgcgctgcgc gagctgggcg cctccttctg ggaggcgaag
     1861 tgggagctcg cgctgccggt cctcgtcgtc gtcctcttcg ccagcggctt cgcctcgatg
     1921 gtcgaggcct ccgccgccgc ctgcgcctac gcggcggtcg tcgagtgcct cgtcacgcgc
     1981 gacatccacc cgttccggca gctcccaggc gtcctcgtga aggccggagc gctcatgggc
     2041 gcggtcctga tcctcctctc gatcgcgatg ggcctgacga gctggctcgt cgacgccatg
     2101 gttccgacgg cgctcctcga gtgggtgaag gggcacgtct cctcgccgct cgtcttcctc
     2161 ctcgtcctga acgggatcct gctcgtcctg ggaagcgtcc tcgagatcta ctcggcgatc
     2221 gtcatcctcg cgccgctcgt cgcgccgatg ggggcggcgt tcgggatcga cccgatccac
     2281 ctcggggtga tcttcctcgc gaacctcgag ctcggcttcc tcttcccgcc cgtcgggctg
     2341 aacctcttcc tctcctcctc ccgcttcggc atcccgctcc cgaagctcta ccgccacgcc
     2401 gtgccgtatc tcctgatcct cggtgcgggc gtcctcctca tcacctacgt cccggcgatg
     2461 tcgaccggga tcctctcgct cctcgggaag aggtagacga aacggcccgc gccggaggcg
     2521 cgggccgctc gggcggcgcg gaggtggccc tgcgctccgg gcgcggcgcg gcgtctcagc
     2581 gcgggtaggg ctgctgctgc tgaggcgtct gcgagatcac gccccattcc aggagccgcc
     2641 tcttcgcgta gtcggcctgc tctccggtcg cgccgctctg gaggatgagc ttgtacttct
     2701 cggccgcctg gccgcggtcg ccgagcgcct cgaggctccg gccgaggtag taggtcgtca
     2761 cgagctgggc gccgacggac ttctccgccg tctcgagctc ggagatcgca tcgcgccagt
     2821 cggagagccg gtaggccgcg agccctccgg cgagccgccc gtggaacagc gccgggtcgc
     2881 gccgggccgc ctcccgtgcc gactcccgcg cggagcgcag ctcgttcgcc tcgacgaggg
     2941 cgatcgcctt gagggcggga aggatcgcct gtcgcggcgc gaggcgggaa gcctcgtcga
     3001 acctcgccgc cgcgccgcgg gcatccttct tcgcgaggag cttctcgccc tcgtccatca
     3061 gcttgaacgc gggcgcctcc gccttcagag cccgggtcga ggcgttgaac gcctcggccc
     3121 gagcgggccg gccgagctgc tccgggtact tctccgcgga gcgccggcgg gccgtctcga
     3181 tccgctccga ggtcagcggg tggctctgga acatcgcctc gagcttcgac ggctccttgt
     3241 cgtgccccgc cttcaggatc tccatcgact cgacgaaccc cttcgggttg tagccggcgc
     3301 gcgccatgta ctccatcccg agctcgtcgc tctggcgctc gtggtctcgc gaataccgaa
     3361 ggaggacgag ctgcccgccg atgccggccg ccgccatgat gagcggcgcg ccgcgcacct
     3421 cctccgcctc caggatcatc ccgccgatgc cgaggagccc tccgatcagc atctgcttcg
     3481 tgtattgctg cgcgccgtga cgcgccgtca cgtgcccgac ctcgtgcccg acgacgcccg
     3541 ccagctggtc ctcggtcgtc atccgggaga ggagcccgcg cgtgatgcag atcttcccgc
     3601 ccggcagggc gaacgcgttg tcgtagttcg cgttgacgac cgtgaactcg tattcgagcg
     3661 ccggacggtg cgagaccttc gcgaggccct gcccgacgcc gtcgaggaac ttctggagtc
     3721 ccgggtcgtt gaccggaccg tacgactcct ggatgagcgg cccgtacgcc tgggcgccga
     3781 gggccttctc ctcctcctcc cccaggaggg agagctgcga cttccctgtg acggggttcg
     3841 tgacgcaggc caccccgaag gcggccgcga cgagagcggc ggcgatacgg cgcgcgagga
     3901 cgttcttcat gtccggatct tgccccttcc gcggcgaatc gtgaaggggc cgggcagccg
     3961 cgcgtgcccg ccggaaaggc ggagggccgc acgcgctcac ggcgcgcgcg gccctccggg
     4021 aggacgcgtc agcccttcga cgcccgggcg cgcgcggcct gctgcgcctg gatcgccgtg
     4081 aggttgatcg tgaagacgat gtcctcgacg aggcacccgc gggagaggtc gttcaccggc
     4141 ttggcgagcc cctggagcat cggcccgatc gagacgacgt tcgccgagcg ctggaccgcc
     4201 ttgtaggtca cgttgccggt gttcaggtcg gggaagatga ggaccgtcgc cttccccgcc
     4261 acgtccgact tgggcgcctt cagcctcgcg acgtcgggca tgaccgccgc gtcgtactgg
     4321 agcgggccgt cgagggcgag gtcgggacgg agctccttcg cgatccgcgt cgcctccttc
     4381 accttctcga cgtcctcgcc cgtgccgctc gtcccggtcg agtaggagag catcgccacc
     4441 cgcgccggga cgccgaacgc gagcgccgag tcggcgctct ggatcgcgat gtcggcgagc
     4501 tccgacgccg tcgggttcgg gttgacggcg cagtcgccga agacgaggac ctggtcgggg
     4561 aggcacatga agaagatgct ggacacgagg ttgcagcccg ggaccgtctt gatgatctgg
     4621 agcgcgggcc ggatcgtgtg ggccgtcgtg tggacggcgc cggagacgag gccgtcggcc
     4681 tcctcgagcg ccatcatcat cgtcccgacc atgatcgcgt cctggagctc cagctcggcc
     4741 cgctcgggag tcatcccctt cgctttgcgc ctctcgacga gcggcgcgat gtagcggtgg
     4801 gcgacggagg cggggttcac gatctccacg gagtcgggca gggtcacacc ctggcggcct
     4861 gcgacgtcac gcacctcgtc ggggttgccg aggaggacgc acttcgcgat gccgcgcgcc
     4921 gcgacgatcg ccgccgccgc gacggtccgc ggctccgctc cctcggggag gacgatccgc
     4981 ttcacgtcgg ctttcgccgt ctccacgagg cggtggcgga aggcgggggg cgagaggcgc
     5041 gggacgcgcg cgggcttcgc gaagccgcgc gcccagccga ggtcgacgtg gtcggcgacg
     5101 aagctcatcg tccgctcgac gcggtcggcg tcgtcgatcg ggatcgtcgg gttcatcgag
     5161 gcgacctgcg cggcggcgcg gtagctcgag gccttgacct ggaggagcgg gagccccccg
     5221 tcgaagacct tccggcagag gtcgaggact ccccggtcgg tcccgagccc ccccgtgagg
     5281 acgacgcccg cgagcggcgt gccgctctgg accgtcatcg cggctgcgag caggatgtcg
     5341 ctccggtcgc ccggcgtcag gacgagcgtc cccggcttca tcgccttcat cgcgttcggg
     5401 acggtcatcg cgcagatcgc gacgtccctc acccggcggg tcgccgcgtc gcccgtgtag
     5461 aggaactggg cccggagcgc gtcgccgatg tccttcacgc ggggggacat caggcccgcg
     5521 ttgcacggga cgatcgcgat cggacggatc ccctccgcct cgagagcggt ctggtgctcc
     5581 ttcaggcatt cgtccgagag cccctgcggc gcgcaggcgc cgcagccgcc ggcgctcggg
     5641 ccgaaggagt cggcgtccac gtccgccggc gcccccttcc ggtcgcagac gcgattcagg
     5701 atcgcgccga cggtccgtcc ctccgagaag gcgccgtagc cgcgcgccgc gatcgcgacc
     5761 gtctccgcga cctcggcggc cgtctggtcg gacggggccg cgacgaggac gagctcggcg
     5821 tcgagagcct tgagcatgag cgcgttcacg cgcgccgagt ggaccatccc catctcgggg
     5881 tgcatcccct ggacgatcag gacgtcgacg tcctcgcccg cctcgttggc gatggcgacg
     5941 acgcgctcca tgagggtctg gtcgtcgccg tcggcgagga gcccctccgc ttccgcgcgc
     6001 ggcaccgccg gcgggatcgc gaagtgggcg ccgagggaga ggagcgggat cgtgtggtcc
     6061 ggctcgcggt tcgcgaccgg cttggcgaag gcgacgcgga tcccctcgcg gtcgagggcc
     6121 cggacgatcc cgaggcaggc ggtcgtcagg ccgacgccgc ggccggcagg ggcgacgagg
     6181 atggtggtgc gcatcttggc ttcccttcct tccgtctcag gcgccgcgcg cgatctgcgc
     6241 ggcgtcctcg gcgatcatga gctcctcgtt cgtcgggacg acgagggcct gcggcttcgt
     6301 gccgatcgtg atccggccgt tctgcgtgcg gccgtgcttc gcgttcgccg cctcgtcgag
     6361 cgcgatgccg aggaatccga ggcgcgcgat cgtcatctcg cggaccgtcg tcgagttctc
     6421 gccgatgccg cccgtgaaga cgagggcgtc gaggcggccg agcgggacga cgagcgcggc
     6481 gatcgccttc gcgaggacgt agcagaagat gtcgagcgcg agcttcgcgc ggtcgtttcc
     6541 gttcgaggcg gcctcctcga ccgtccgcat gtcgttcgag aggcccgaga cgccgaggag
     6601 ccccgacttc ttgttcaggg cgtcgaggac ctccttcgcg ctctttcccg tcgcgtcggc
     6661 cacgtggccg acgatcgccg gatcgatcga gccgcagcgg gtccccatca tcagcccctc
     6721 gagcggcgtc aggcccatcg tcgtgtcgac gctcttcccg ttccggatcg ccgccgcgga
     6781 gcagccgttc ccgaggtggg cggtgacgat cgtgtggtcg tcggccggga gcccggcgag
     6841 ccggagcgcc tcccgggcga cgaagcgatg gctcgtcccg tggaagccgt agcgcctcac
     6901 gccgtgctgc tcgagccact cgtacggcac cgggtaggtg aaggcgacgg gcggcatcgt
     6961 ctggtggaag gccgtgtcga agacgccgac ctggaggagg tccgggaaga ggttctgcgc
     7021 gatccggatc ccgacgaggt tcggcgggtt gtggaggggc ccgagcggga tgcactcctc
     7081 catcttcgcg acgacctccg gggtgatcgc catcgagccg gcgaacttgg agccgccgtg
     7141 gacgacgcgg tgcccgacgg cgaggaggcc gtccgcgagg ccgagctcct tcagggcgtc
     7201 gacgacgccg tggagggctt cgtcgtggag ggcgcccggg atcggccgcg agctcttcgt
     7261 gccgttccgc ttgacgtcga gcgacgcctc gggcgagccg agccgctgcg cgatgccgga
     7321 gacgtgctcc gcggaggtgt cggggtcgat gaccgcgaac ttgagggacg aggatccgca
     7381 gttgaggacg agtacgttca tgcccttctt cccgtgccgg ccgagcccgg ccgacggcgg
     7441 gagaacgtct cccgcgcatc cctccgagcg gcgcaccggc gtgtcgtttc gcttctgatg
     7501 tcggactccg atgccgcctc ccggcgcccg ccggaaacgt ctccggggcg cccgcgaggc
     7561 gagcggagca tcataacgcc cgaaggggct ctcgagccgt gatggccctc ccgcggttct
     7621 tcgtcgcgac tcggttccaa cttcgcggcc gcgcaccggg aaccttcccg cccgcggaag
     7681 gtctcatcgg gcggaggacg ccgatgagaa cccgcaccgc cgccgcgctc gccctggcct
     7741 tcgccgcctc gcttcccgcc gccgccgcca cccgcggcgg ctacgagctc tccgtcctcg
     7801 tcgacggctc ggagcggtcc gagctccgga agggcggcgc cgtctacgtc gaggcgctga
     7861 aggggcgcga gtacgcactg cgcgtcacga acccgctcgg cgtccgcgtc ggggtcgcgc
     7921 tcgccgtcga cggtctcaac acgatcgacg ccacgcaccg cgacgccgcc tcggccgtga
     7981 aatgggtcct cgagccgtac cagacgatcg tcctcgaggg ttggcaggtc agctcctcgg
     8041 acgcccgccg tttcgtcttc accggggagc gcgactccta cggcgccgct ctcgggaaga
     8101 cggaggacct cggggtgatc gaggccgtct tctttccgga gaaagtccgc cggcctgccc
     8161 ccgtgtcgcc gatggcctgc gacgggcggg actcctcccg gcagtcggcc ggcgcgcctc
     8221 cggcgccgcg gtcgaagggc gaaggggccg gcggccgcgc ggaggccgag aagtccgcgg
     8281 cctcggccct ctccgacgag tacgccgcga ccggaatggg cgatcggacg cgccacgagg
     8341 tggcccgcgt cgacctcgac ctcgagagga ctcccgcggc ccgcatccgg atccgctacg
     8401 agttccgccc gcagctcgtc cgcctcggcc tcctcccgcc cgaaggggac cgcctcgccc
     8461 gtcgcgaacg agcccgtggt ttcgaccgct actgccccga accggatcgc tgagctcgaa
     8521 agcccggaat tcgtcccgcc tcgccgcccg cctcgccccg gggtcctcgc cccggggcgc
     8581 ttcgcgtccg gacccgctac ccgagcatcg accggcatcc cgcccgtccg cccttgaccg
     8641 aaaatcgcgg cgcaaatatc ctattaatcc agtaggaata ctttgaaagt ttcccagcgc
     8701 ggtctctacg ccctgaaagc gctcctccac ctggcggaac ggtgggacgg cggaaccgtg
     8761 tcgacacgcg agatcgccga gcgcgaggag atcccggaga ggttcctcga gtcgatcctc
     8821 gtcctcctga agaacgcccg catcgtcacg agccggcggg gacgggaagg gggctatcgc
     8881 ctccgacgtc ctccggccga gatcgtcgtc ggcgacgtcg tccggctcgt cgacggcccg
     8941 ctcgcgcctt tcggcgacgc cgtcgagctc gcccggctcg tcgaatccga gccccggcac
     9001 gccggcctct tcgagctctt cctcgacctg aggaacgccg cctccgcgat cctcgaccac
     9061 acgtctctcg ccgacctcct cgagcgcgac cggaggatcc tcgtcgcgcg cgccgagagg
     9121 gcccccggcg ccacggtgaa gcagtgaacc gttcctcccg ccgcttcctc cgtcccgtcc
     9181 tcgctttcgc cgcgtccgtc gccgcgattc tctcgatcca cgccaggggc ggcgccgcgc
     9241 cacccgcgcg ggtcgagctc ctgaacgtct cctacgaccc gacccgcgag ctctaccggg
     9301 aagtgaacgc cgccttctcg agggagtacc tcgcgaagac cggtacggcg gtcaccgtta
     9361 agatgtcgca cggcgggtcg gggaagcagg cccgctcggt gatcgacggg ctcaaggccg
     9421 acgtcgtcac gctcgccctc gcgtacgacg tcgacgcgct cgccgaccgc ggcctcgtga
     9481 ggcccgggtg gcaggcgcgc ctcccgcaca actcgtcccc gtacacgtcg acgatcgtct
     9541 tcctcgtgag gtccgggaac ccgaagggga tccgggactg gaccgacctc gcgcgccccg
     9601 gcgtctccgt catctgcccg aacccgaaga cctcgggcgc cgc
//
LOCUS       DAOTEZ010000136         9586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5035_length_9586_cov_6.888574, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000136 DAOTEZ010000000
VERSION     DAOTEZ010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9586
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5035_length_9586_cov_6.888574"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="P5164_08760"
     CDS             complement(<1..892)
                     /locus_tag="P5164_08760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY44021.1"
                     /translation="MSGPGAGVVLLGPVPPWRSGIADQTVRLARAMTRLGAPPLVITF
                     SRMYPRALYPGAADRGEGAFPGDVEVVPLLDGFDPLSFRRAAALAASRGARLAAAAFW
                     TSVFAPHLALFLSAHRAEAPDAVRLLLCHNLADHEAGPLRRALARSALRRADVLAVQN
                     AGDLATAQREVPEPRAVLVPHPAEPVPRVPRAEARRLLDLPEGAPVFLFSGLLRLYKG
                     WDVLLEAFSVVRRELPSALLVLAGEPWGEAKALRERSAPDGVLLRLRFLPPDERWLLF
                     DACDAVVCPYRHATGSGIAGD"
     gene            complement(889..2058)
                     /locus_tag="P5164_08765"
     CDS             complement(889..2058)
                     /locus_tag="P5164_08765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HRY44022.1"
                     /translation="MTTTLLLRAGRDDAARRRHPWIFSRAIAGSPLAGEVEVRSASRQ
                     LLGRGFASPRSSIAARLWRCGEGPLDAPYVRGLVVEAVRLRERFMPAGTDGYRVVHAE
                     GDGLPGLVVDRYADVLVVQATTEGTDAARPLWLPPLRELFPAASILQRNDLASRHGEG
                     LSLDDELLAGKRPPGSVPFREHGLTFRADLAGGQKTGFFLDQRENRNLVRGLSSGRSV
                     LNLFSYSGAFGVAALAGGASRVTHVDVSEPALALARENHDANGQDPRRVETVAADVFE
                     DLRARVGRGETWDVVVTDPPAFAKKTADVDRACRGYKDVNRLALKLLAPGGLLLACSC
                     SGPVSADLFQKVLFAAALDAGIPARILERRGAGPDHPVSIDCPETEYLKAFLLSR"
     gene            2113..3270
                     /locus_tag="P5164_08770"
     CDS             2113..3270
                     /locus_tag="P5164_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRY44023.1"
                     /translation="MELLLALLLAVAVVALASAWSALKTERGRCRRLEDRLAAVRPEA
                     SALPESPSPALPEPPPAATSNPPRAAAAPLAAPPEPVPFGRVSAFENLAATLLGPAER
                     LADSMRAAEPFLADAAGRLRAPLPPSPPVLAGDDPAPPRIDVDGLERALATAERISDE
                     RSRLADDVRSLRAALDRVAELDTGLARGLSELSRTAEALMPLAASVSGLADRANLLGL
                     NVSLLSARAGEAGAPFEEAATELRSLFEEARRLSRELIETARRNDGGARRVAALVEES
                     VGAATAGQERGARASERLGGLDGLCSQLGRALEDGVRSTRGAAESSRLVCERLEAVRS
                     ALEGRTSEAARLRADADAVREALRIAAERVEALRLDGGALRAAVQRVTSGA"
     gene            3306..3842
                     /locus_tag="P5164_08775"
     CDS             3306..3842
                     /locus_tag="P5164_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY44024.1"
                     /translation="MPTDTLTPETVLAEWRSGNLEDLVELGVSAARAGEYERGLIFLA
                     EAYRHLSREKHRLSGPLLSSYGLCLALHKGRIKEAAEFCWLGVEKDHFNPDAYYNMAR
                     VWMAGRSRRKAVEALEKGLALDPRHPGLLRLRAEFGTRQPPVISFLHRDNPLNVSLGR
                     MRKRIAAKKSSPASRSGR"
     gene            complement(3856..4491)
                     /locus_tag="P5164_08780"
     CDS             complement(3856..4491)
                     /locus_tag="P5164_08780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HRY44025.1"
                     /translation="MSLLIAGLDDYLLSVLPSRDRVLARIEEDAARRRIPIVGPAVGR
                     LLAQYVHLTGARRVFELGSAIGYSTIWLARAVGESGQVFWTDASEENAAEARRNLEEA
                     GVADRVALLVGDALASLDATEGEFDLVFNDVDKEGYPDVLRRAVPRLRVGGLLVTDNV
                     LWDGALRLPACEDDAETAAIREFNRLCYERPDLETTILPLRDGVSVVRRIR"
     gene            complement(4530..5090)
                     /locus_tag="P5164_08785"
     CDS             complement(4530..5090)
                     /locus_tag="P5164_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44026.1"
                     /translation="MVPLTSLWIPILVSAALVFVVSSVIHMLLPVHKNDFRKLPKEDE
                     ALEAMRKLGLTPGDYMFPLCSSMAEMKDPAFLGKMEKGPVGVLTVMPSGPPAMGPSLA
                     LWFVYCAVVGLFAAYVAGRALPVGAQSLAVFRFAGVTAFVGYSLALWQNTIWYKRSAV
                     STLKSTIDGLVYALCTGGVFGWLWPR"
     gene            5301..6530
                     /locus_tag="P5164_08790"
     CDS             5301..6530
                     /locus_tag="P5164_08790"
                     /inference="COORDINATES: protein motif:HMM:NF015039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HRY44027.1"
                     /translation="MRRCTPALAALLAIFASPVAAGERRDGVVFAEKVDARLDAIRAE
                     VRTKETPPPKKRMWVDFSAVDAPKSASEFTSVWHQPPVCQGLSGMCWCFSTTSFLESE
                     VHRLTKRELRLSVLHTVYWEHVEKAKGWVRSRGTSVYGEGSQPAAVLRALREHGAVPA
                     EAYTGLADGRKEHDHEATLYAEIRAYLEGVKAAGAWNEAAVVSTVRAILDHWLGVPPE
                     TVVVEGVAMTPKEYLAKVVRLDPDDYVAFLSTMEQPYWEQAEYAVPDNWWHGKEYVNV
                     PLDDFLAAIRGAVRGGFSVVVAGDMSEPGYSIGPPGIAVVPTWDVPSAFIDEAARAFR
                     FANGSTTDDHGLHVVGVTSRRGKDWFLVKDSWSSSWNNDHPGYYFFHEDYVKLKMLAF
                     LVHRDAVKELLARVKKG"
     gene            6556..8775
                     /locus_tag="P5164_08795"
     CDS             6556..8775
                     /locus_tag="P5164_08795"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRY44028.1"
                     /translation="MRAVRLPAALLALLALAAFSAWRSSPPSPRATDAPATEFSAERA
                     AVLLERLLGGGAPRTPGSAAHAAFREHLLDEVRRLGLTPETEEGFACAPRGSCARLVN
                     VLAELPGTTGGPAVLLTAHTDSVGAGPGAGDDASGVAVLLEAARALLATPRRNPVLLL
                     FDDGEEDGLLGAELFVRSPRARAIGAVVNLEARGTSGPSLLFETSAQNAWLVRSAVSR
                     LPRPVTSSLFAFVYDRLPNDTDLTVYKREGLAGLNLAFLGSAARYHTREDSLANLSRA
                     SLQHQGENALALARALAETDLREPPSGRAVFFSVAGAFVVAWPREAAPAVALLSLLLV
                     RLACLRRGGHLLPRPRAAVPSLAPFAAALSGLLAGQLLRVLGAFPVTFVPRPWASLAA
                     AVAVSLLAALPVLSAESRRAGDAVDWWERAWTSIALLGIVLALAWPETSYLLVAPALV
                     AGAARLALPEPVSPWPGFAASGLVLLPLALILPDALGPVALPVVAAAFGIVLATIPVD
                     GTPGAWRRPAVALAALFVAASVAALVFSKPTPDEPEHGTMIAVQEEGTPEARLAYSPA
                     SGRLHPSVAAAARFERAPGLFPWARKAAPFAAPLPPGAEPFPAAELLSSTAGPEGRRR
                     LRIRLTSPRGASRLELLVPDAKGVEEIRVEGRSLPAPYLRRKAATRRPIRLSIRAAPP
                     EGIVVDLTLPAAPREAFVLDETPGLPPSAVALGRSRPATVAPANGGDATILVRKLSL"
     gene            8978..>9586
                     /locus_tag="P5164_08800"
     CDS             8978..>9586
                     /locus_tag="P5164_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HRY44029.1"
                     /translation="MLGVVERAGNALPHPATIFLLLALAVLGASAIGSWAGLTAVHPG
                     TGKAITVVNLLTVEGLHLVLEKAVSNFTSFAPLGTVLVALLGIGVAEGSGLIGTLLRL
                     LVLSAPRRLLTFVVVFAGVMSNAASEVGYVLLIPLAAVIFLAAGRHPLAGLAAAFAGV
                     SGGYSANLVLGTIDPLLAGLTQEAAHIVDPSYTVSPAANYYFL"
BASE COUNT         1237 a         3685 c         3376 g         1288 t
ORIGIN      
        1 cgtcgccggc gatcccggag ccggtcgcgt gccggtaggg gcagacgacc gcgtcgcacg
       61 cgtcgaagag gagccagcgc tcgtcgggcg ggagaaagcg gaggcgcagg aggacgccgt
      121 cgggcgcgga ccgctcccgg agcgctttcg cctcccccca cggctcgccc gcgaggacga
      181 ggagcgcgga agggagctcc cggcgaacga cggaaaacgc ctccagaagg acgtcccagc
      241 ccttgtagag gcggaggagg ccgctgaaga gaaagaccgg agcgccctcg ggaaggtcga
      301 ggagcctccg ggcctcggca cgcgggacgc gcgggacggg ctccgcgggg tgcggcacga
      361 ggacggcccg gggctccggg acctcccgct gcgccgtcgc gaggtctccc gcgttctgga
      421 cggccaggac gtccgcccgc cggagagcgg agcgcgcgag ggcgcgccgg agaggcccgg
      481 cctcgtggtc cgcgaggttg tggcagagga ggaggcggac cgcgtcgggc gcctcggcgc
      541 ggtgcgccga gaggaagagc gcgaggtgcg gcgcgaagac cgaggtccag aacgccgccg
      601 cggcgagccg ggctccgcgc gaggccgcga gggcggccgc gcgacgaaag gagagcggat
      661 cgaagccgtc caggagcggc accacctcga cgtccccggg gaacgccccc tctccgcgat
      721 ccgccgctcc gggatagagc gcgcgcgggt acatccgcga gaacgtgatc acgagagggg
      781 gcgcgccgag ccgcgtcatc gcgcgcgcga gccgcaccgt ctggtcggcg atcccgctcc
      841 tccacggggg gacgggaccg aggaggacga cgcccgcgcc gggcccgctc atcgggagag
      901 gaggaacgcc ttcaggtact cggtctcggg gcagtcgatc gagaccgggt ggtccggacc
      961 ggcgccgcgc cgctccagga tcctcgcggg aatgccggcg tcgagcgcgg cggcgaagag
     1021 gaccttctgg aagaggtctg cggagacggg ccccgagcag gagcaggcga ggaggaggcc
     1081 cccgggggcg aggagcttca gggcgaggcg gttcacgtcc ttgtagcccc ggcaggcccg
     1141 gtcgacgtcc gccgtcttct tcgcgaaggc gggcgggtcg gtcacgacga cgtcccacgt
     1201 ctcgccccgt ccgacgcgag cgcggaggtc ctcgaagacg tccgcagcga cggtctcgac
     1261 ccggcggggg tcctggccgt tcgcgtcgtg gttctccctc gcgagcgcga gcgccggctc
     1321 cgagacgtcg acgtgggtca cgcgcgaggc gccgccggcg agcgccgcca cgccgaacgc
     1381 gcccgagtag gagaagaggt tgaggacgct ccggccgctc gagagaccgc ggacgaggtt
     1441 ccggttctcc ctctggtcga ggaagaagcc ggtcttctgc ccgcccgcga ggtcggcacg
     1501 gaacgtgagg ccatgctcgc ggaacgggac gctcccggga ggcctctttc cggcgaggag
     1561 ctcgtcgtcg agcgagagcc cctcgccgtg ccggctcgcg aggtcgttcc gctggaggat
     1621 cgacgccgcc gggaagagct cgcggagcgg ggggagccag aggggccgcg ccgcatccgt
     1681 cccctccgtc gtcgcctgga cgacgaggac gtccgcgtac cggtcgacga cgagtccggg
     1741 gaggccgtcc ccctccgcgt gaacgacccg atagccgtcg gtcccagccg gcatgaagcg
     1801 ctcccgcagg cggacggctt cgacgacgag gccccggacg tacggcgcgt cgagcggccc
     1861 ttccccgcac cgccagagcc gggcggcgat ggaggagcgg ggggacgcga acccgcggcc
     1921 gagcagctgc cgggacgccg aacggacctc gacctcgccc gcgagcgggc ttcccgcgat
     1981 cgcccgcgag aagatccacg ggtgccggcg gcgggcggcg tcgtcgcggc ccgcgcggag
     2041 gagaagggtc gtcgtcaaac cggatctgga gtttaggcgg gagcgccggg ccggtgtacg
     2101 ctttccctcc ggatggagct tcttctcgcc ctcctcctcg ccgtcgcggt cgtcgctctc
     2161 gcgtccgcct ggagcgccct gaagaccgaa cgggggcgat gccggcgcct cgaggaccgg
     2221 ctcgccgccg tccgcccgga ggcctccgcc cttccggagt ctccttcgcc ggcgctgccg
     2281 gaacccccgc ccgccgcgac ctccaatcct ccgcgagcag ccgccgctcc cctcgcggca
     2341 ccgccggagc ccgtcccctt cggccgggtc tcggccttcg agaacctggc cgccacgctg
     2401 ctcggacccg cggagcgcct cgccgactcg atgcgtgccg ccgaaccgtt cctcgccgac
     2461 gccgccggcc ggctccgggc cccgctcccg ccctccccgc cggtgctcgc cggcgacgac
     2521 cccgccccgc ctcgaatcga cgtcgacggc ctcgaacggg cgctcgccac ggccgagcgg
     2581 atctcggacg agaggtcccg cctggcggac gacgtccgca gcctccgggc ggccctcgac
     2641 cgcgtcgccg agctcgacac cggactcgcc cgcggcctct cggagctttc gcgcaccgcg
     2701 gaggcgctca tgccgctcgc ggcctcggtc tccggcctcg ccgaccgcgc gaacctcctc
     2761 ggcctgaacg tctctctcct gtcggcgcgc gccggcgagg cgggcgcgcc gttcgaggag
     2821 gcggcgacgg agctccggag cctgttcgaa gaggcccgtc gcctctcgcg cgagctgatc
     2881 gagaccgcgc gccggaacga cgggggcgcc cggcgtgtcg ccgccctcgt ggaggagtcc
     2941 gtcggcgccg ccacggccgg ccaggagcga ggcgcgcggg cctcggagcg tctcggcgga
     3001 ctggacggcc tctgctccca gctcggccgc gcgctcgagg acggggtccg gtcgacccgg
     3061 ggcgccgccg agtcctcgcg tctcgtctgc gagaggctcg aggcggtccg gtccgccctc
     3121 gagggccgga cctcggaggc ggcgcggctc cgggccgacg cggacgccgt gcgggaggcc
     3181 ctccggatcg ccgccgagcg ggtcgaggcg ctccgcctgg acggcggcgc cctgcgcgcc
     3241 gccgtccagc gcgtcacttc cggcgcttga cacgcccggc gcgggttccc agactgccga
     3301 ggcccatgcc gaccgacacg ctcacacccg aaaccgtcct cgccgaatgg cgctcgggca
     3361 acctcgagga cctcgtcgag ctcggggtct ccgcggcccg cgccggcgag tacgagagag
     3421 gcctgatctt cctcgccgag gcctatcgcc acctcagccg cgagaagcac cgcctctcgg
     3481 gcccgctcct gtccagctac ggcctctgcc tcgcgctcca caagggacgc atcaaggagg
     3541 ccgccgagtt ctgctggctc ggagtcgaga aggaccattt caaccccgac gcctactaca
     3601 acatggcgcg cgtctggatg gcgggccgga gccggcgcaa ggcggtcgag gcgctcgaga
     3661 aaggtctcgc cctcgacccg aggcaccccg gcctcctgcg tcttcgcgcg gagttcggca
     3721 cccgccagcc gccggtgatc tccttcctgc accgcgacaa cccgctcaac gtctccctcg
     3781 gaaggatgcg aaagcggatc gccgcgaaga agtcctctcc ggcgtcccgt tccggccgct
     3841 gacgatcggc ccgcctcagc ggatccggcg tacgacggag acgccgtccc ggagcgggag
     3901 gatcgtcgtc tcgaggtccg ggcgctcgta gcagagccgg ttgaactcgc ggatcgccgc
     3961 cgtctcggcg tcgtcctcgc acgccgggag gcggagcgcc ccgtcccaga ggacgttgtc
     4021 ggtcacgagg agccccccga cgcgaagccg gggcaccgcg cgccggagga cgtcgggata
     4081 gccctccttg tcgacgtcgt tgaagacgag gtcgaattcg ccctccgtcg cgtcgaggct
     4141 cgcgagcgcg tcgccgacga ggagcgcgac acggtcggcg acgcccgcct cctcgaggtt
     4201 ccgccgcgcc tctgcggcgt tctcctccga cgcgtcggtc cagaagacct gccccgactc
     4261 tccgacggcg cgcgccagcc agatcgtcga gtagccgatc gcgctgccga gctcgaagac
     4321 cctccgcgct cccgtcaggt ggacgtactg cgcgaggagt cggccgacgg ccggcccgac
     4381 gatcgggatc cggcgccgcg cggcgtcctc ctcgatccgc gcgaggacgc ggtcccggga
     4441 agggaggacg gagaggaggt agtcgtcgag cccggcgatg agaagcgaca tggcgccgga
     4501 gtgtagcggc cggtccggcc gctcccgagt caccgcggcc agagccagcc gaagaccccg
     4561 ccggtgcaga gggcgtagac gagcccgtcg atcgtcgact tcagcgtcga aacggccgag
     4621 cgcttgtacc agatcgtgtt ctgccagagc gcgagggagt agccgacgaa cgccgtgacg
     4681 cccgcgaaac gaaagaccgc cagggattgc gccccgaccg ggagcgccct ccccgcgacg
     4741 taagccgcga agagcccgac gacggcgcag tagacgaacc agagcgcgag gctcggcccc
     4801 atcgcgggcg ggccgctcgg catcaccgtg agcacgccga ccggcccctt ctccatcttc
     4861 ccgaggaagg cgggatcctt catctcggcc atgctcgagc agagcgggaa catgtagtcg
     4921 ccgggcgtga gaccgagctt cctcatcgcc tccagcgcct cgtcctcctt cgggagcttc
     4981 cggaagtcgt tcttgtgcac cgggaggagc atgtggatga cggagctgac gacgaagacg
     5041 agggccgcgg agacgaggat cgggatccac aacgaggtca gagggaccat cggggcctcc
     5101 tgcagcgaaa tctcgatttc gtcgggagca tccccgatcc gcgccgccga agtccacttc
     5161 ccgccgatcc gagcccgtcc ggccgcctct cccgcgtccc cgtgagaaca atgcacgatt
     5221 tcacgcctgg caccggtgga cgtggcaccg gtggacggcc gcggtcgatc gcgccagact
     5281 gcgccgtccg gaggtcctcg atgagacgtt gcacgcccgc cctcgccgcc ctgctcgcca
     5341 tcttcgcgtc gccggtcgcc gccggggagc gccgggacgg cgtcgtcttc gccgagaagg
     5401 tggacgccag gctcgacgcg atccgggccg aagtgcggac gaaggagacg cccccgccga
     5461 agaagcggat gtgggtcgac ttctccgccg tagacgcgcc gaagagcgca tcggagttca
     5521 cgagcgtctg gcaccagccg ccggtctgcc aggggctttc ggggatgtgc tggtgcttct
     5581 cgacgacctc gttcctcgag tcggaggtcc accggctcac gaagagagag ctccggctct
     5641 cggtcctcca caccgtctac tgggagcacg tcgagaaggc gaagggctgg gtccgttcgc
     5701 gcgggacgtc ggtctacggc gagggctcgc agccggcggc cgtcctccgt gcgctgcgcg
     5761 agcacggcgc cgttcccgcc gaggcctata cgggcctcgc ggacgggcgg aaggagcacg
     5821 accacgaggc gacgctctac gccgagatcc gggcgtacct cgagggcgtg aaggccgcgg
     5881 gggcgtggaa cgaggccgcc gtcgtctcga ccgtccgcgc gatcctcgac cactggctcg
     5941 gcgtcccgcc cgagacggtc gtcgtcgaag gggtggcgat gacgccgaag gagtacctcg
     6001 cgaaggtcgt gaggctcgac cccgacgact acgtcgcgtt cctctcgacg atggagcaac
     6061 cgtactggga gcaggccgaa tacgccgtcc ccgacaactg gtggcacggg aaggagtacg
     6121 tgaacgtccc gctcgacgac ttcctcgcgg cgatccgggg agccgtgcgg ggcggcttct
     6181 ccgtcgtcgt cgccggcgac atgtcggagc ccggctactc catcggccct cccggcatcg
     6241 ccgtcgtccc gacgtgggac gtcccctccg ccttcatcga cgaggcggcg cgcgcgttcc
     6301 gcttcgcgaa cgggagcacg acggacgacc acgggctcca cgtcgtcggc gtgacgagcc
     6361 ggagagggaa ggactggttc ctcgtgaagg actcctggtc gagctcctgg aacaacgacc
     6421 atcccggcta ctacttcttc cacgaggact acgtgaagct caagatgctc gcgttcctcg
     6481 tccacaggga cgcggtgaag gagctcctcg cgcgcgtgaa gaagggctga gccccgcttc
     6541 tgggctcgac gtccgatgcg tgccgtccgg ctccccgcgg ccctcctcgc cctcctcgcc
     6601 ctcgcggcct tctccgcctg gcgatcctcg cccccctcac cgcgcgcgac cgacgcgccc
     6661 gcgacggagt tctcggcgga gcgggcggcg gtgctgctcg agcgcctcct cggcggcggg
     6721 gcgccgcgga cgcccgggag cgcggcgcac gccgcgttcc gcgagcacct gctcgacgag
     6781 gtccggcggc tcggcctgac gccggagacg gaggaaggct tcgcctgcgc cccgcgcggc
     6841 tcctgcgcgc ggctcgtgaa cgtcctggcc gagctgcccg gaacgacggg aggccccgcg
     6901 gtcctcctca cggcgcacac cgactccgtc ggcgcgggcc cgggggcggg cgacgacgcg
     6961 tcgggagtcg ccgtcctcct cgaggcggcc cgggccctcc tcgccacccc gcggcggaac
     7021 cccgtcctcc tcctcttcga cgacggcgag gaggacgggc tcctcggcgc ggagctcttc
     7081 gtccgctccc cgcgggcgcg agcgatcggc gccgtcgtga acctcgaggc gcgcgggacg
     7141 agcggcccga gcctcctctt cgagacgagc gcgcagaacg cgtggctcgt ccggagcgcc
     7201 gtctcccgcc tcccgcgccc cgtcacgagc tcgctcttcg ccttcgtcta cgaccggctc
     7261 ccgaacgaca ccgacctgac ggtctacaag cgggaggggc tcgccgggct caacctcgcg
     7321 ttcctcggct ccgcggcgcg ctaccacacg cgcgaggact ccctcgcgaa cctttcgcgc
     7381 gcctccctcc agcaccaggg ggagaacgcc ctcgccctcg cgcgcgccct cgcggagacc
     7441 gacctgcgcg agccgccctc cggccgagcc gtcttcttct ccgtggcggg agcgttcgtc
     7501 gtcgcctggc cgcgggaagc ggccccggcc gtcgcgctcc tctcgctcct cctcgtccgc
     7561 ctcgcctgcc ttcgtcgcgg cgggcatctc ctcccgcgcc cccgcgccgc cgtcccgtcc
     7621 ctcgctccgt tcgccgccgc cctctccggc ctcctcgccg ggcagctcct ccgggtgctc
     7681 ggcgccttcc ccgtcacgtt cgtcccgagg ccgtgggcct ctctcgcggc cgccgtcgcg
     7741 gtctctctcc tcgccgcgct tcccgtcctc tccgcggagt cgcgccgggc gggcgatgcc
     7801 gtcgattggt gggagagggc ctggacctcg atcgcgctgc tggggatcgt tctcgcgctc
     7861 gcgtggcccg agacctccta cctcctcgtc gcccccgcgc tcgtcgccgg cgccgcgcgc
     7921 ctcgcgctcc cggagcccgt ctccccctgg ccggggttcg cggcctccgg cctcgtcctc
     7981 ctcccgctcg cgctcatcct ccccgacgct ctcggccctg tcgccctccc ggtcgtcgcc
     8041 gcggcgttcg ggatcgtcct cgcgacgatt ccggtcgacg ggacgcccgg cgcctggcgc
     8101 cgtcccgccg tcgccctcgc cgccctcttc gtcgccgcgt ccgtcgcagc cctcgtcttc
     8161 tcgaagccga cgcccgacga gcccgagcac ggcacgatga tcgccgtcca ggaggagggg
     8221 acgccggagg cgcgtctcgc ctacagcccc gcctccgggc ggcttcaccc ttcggtcgcg
     8281 gccgcggccc gcttcgagag ggcgcccggg ctcttcccgt gggcaaggaa ggccgcaccg
     8341 ttcgcagcgc cgctcccccc gggggccgag cccttcccgg ccgcggagct cctctcctcc
     8401 acggccgggc cggaaggccg aagacgcctc cgcattcgcc tcacctctcc gcgcggggcg
     8461 tcgcgcctcg agctcctcgt ccccgacgcg aaaggcgtcg aggagatccg cgtcgaggga
     8521 cgatctctcc ctgcgcccta tctccggagg aaggccgcga ccagacgccc gatccggctc
     8581 tccattcgcg ccgccccgcc ggagggcatc gtcgtcgacc tgacgctccc ggctgcccct
     8641 cgcgaggcgt tcgtcctcga cgagacgccg gggcttcctc cgtccgcggt ggcgctcggc
     8701 cggtcccgcc ccgcgacggt cgctcctgcg aacggcggcg acgcgacgat cctcgtccgg
     8761 aagctctccc tgtgagggat cgcctctccc ggctcttcga cggtcctccg cccccgggaa
     8821 atctcgcggc cccgggaata cctgtccctc tcgcggcgtt gagatcccat cgcgtcgggc
     8881 ggcacgccgg gcagagaggt gacgagtggg gacgaagaac gaacggagcc gcgccgcggc
     8941 gaagccggtc ggcgtccggg gggacctctt ctcccggatg ctcggggtcg tggagagggc
     9001 ggggaacgcg ctcccccacc ccgcgacgat cttcctcctc ctcgcgctcg ccgtcctcgg
     9061 cgcctcggcg atcggctcct gggccgggct cacggcggtc caccccggga cgggcaaggc
     9121 gatcacggtc gtcaacctcc tgacggtcga aggcctccac ctcgtcctcg agaaggccgt
     9181 ctcgaacttc acgagcttcg ccccgctcgg caccgtcctc gtcgcgctcc tcggcatcgg
     9241 cgtcgcggag gggagcggcc tcatcgggac gctcctccgc ctcctcgtcc tctctgcccc
     9301 gcgccggctc ctgacgttcg tcgtcgtgtt cgcaggggtc atgtccaacg cggcgagcga
     9361 ggtcggctac gtcctcctca tcccgctcgc ggccgtgatc ttcctcgccg cggggcggca
     9421 cccgctcgcc ggcctcgccg ccgccttcgc cggggtctcg ggcggctaca gcgcgaacct
     9481 cgtcctcggg acgatcgacc cgctcctcgc cggcctcacg caggaggccg cgcacatcgt
     9541 cgacccctct tacaccgtca gccccgccgc gaactattac ttcctc
//
LOCUS       DAOTEZ010000137         9561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5054_length_9561_cov_5.204713, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000137 DAOTEZ010000000
VERSION     DAOTEZ010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9561
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5054_length_9561_cov_5.204713"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..1952
                     /locus_tag="P5164_08805"
     CDS             168..1952
                     /locus_tag="P5164_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRY44030.1"
                     /translation="MTTPSTLVDVFRNLGAMPKPDLLLYKKDGRWVPISTAEFTDRVK
                     AVSAALGTVGVPDGGAVVLLSENRPEWPTIDFACQCYGAVLVPVFPTMVADQAEFIVR
                     DCGATVAFASTPDQAAKLLKAQATCPKLKHVFVFDHAGGGGKPFGALVEEGRKAHAAS
                     PDLFDERAGRRKASDLATIIYTSGTTGEPKGAMLMQSNFVSNMLAGCSLLPFDSTAVT
                     MSFLPLAHVFERLVEYCYYHRGATIAFAESIDKLRDNLGEVNPHIFGAVPRVYEKVYA
                     RVMDKVDAGSPAKQRLFRTAIGVGKEVLRLRTEKKTPGGALALQHFLYERLVFRKIRE
                     ALGTRFRFAISGGAPLAKDLAEFFWAIGVEVYEGYGLTETSPVIAVNCPSAWKLGTVG
                     RVMPGVEVRIADDGEILTRGPHVMKGYYGRPEATAETIDADGWLHTGDIGLLDEEGFL
                     RITDRKKEIIVNSNGKNVAPAPIESALKGDPFVAQPVVIGDRRKFLSCLIVPNFEKLR
                     EWAAKNGLGSLSDEELVAHPRVEQIYQGTVDRWNEGKPNEQLIRAFKLLPKELTIEGG
                     ELTPTLKVKRRIVDQKFRSVIDGLYKGD"
     gene            complement(2007..6947)
                     /locus_tag="P5164_08810"
     CDS             complement(2007..6947)
                     /locus_tag="P5164_08810"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRY44031.1"
                     /translation="MSTALRSSSRLVLPTFLVLAVWLFHPRPAAAQSWPMFQGNPAHT
                     GYVPVSLEPQWFHVRWTRNLGTIPNFTLPVNPVAAGDGRVYATLLTRFNDVPQLFALR
                     TSDGATLWTKGFGAVFSVNPPSYVNGRVYVQTGNSSDTWLWAFDGSSGTTIFQSPHGA
                     QWERYFSPTVSNGRVYVNGGEYGGMYAFSALNGDQFWYADLPQYHEWTPAVDGTSAYA
                     YVGSYAPGLYIKNLLDGTASTPGFIPDPDFEWDDWSMNLAPVLGAYGVLAIHNGRLIS
                     FNTMAGTIGWQLSEGFTGQPSVAKGRIYAANNGGLSVYDESSHTLVWSWTPPPAEPKQ
                     AITQPMIVTDTHVLVSTRDTVYAIDLTTHEAVWTFAAPYGELAIADGRLFVAAHDGTL
                     TAISLTSSAKPVSISPTSGPSLGGTPVAISGSGFQTGAVVRIGGIPAVVTSVASNLIN
                     AITGPHVNGLVDVEVENPDGDVGRIRNGFTYVCGGTAPTASVSGSGTVCIGESATLSA
                     ALTGSGPWNLVWSDGFFQNGIATSPATRDVTPGATTTYTLRTVTAGLCAGTASGSATI
                     TVWSTSNATLTAPARVCVGTNATASIPATTGALYNWTVTNGTIVSGQGTNVLSFVGTT
                     NPVTVSVIATIGSCSATASRDLPVSLPPSATVTSGWSVCAGETALVSFVLHGVPPFTV
                     RWSDGVVQSVGAAGPETRTVSPATSTIYAISSLTDAYGCAGGRASGSAPVIVNPVPSA
                     SISAPGSYCAGAADVAASVPDAGGGATYESTIAGGTIAGSTSGRSVTFTPTGAAVTLG
                     VTVTLPSGCRASSTRVSSLAPAPSATVSGGGTICAGNAATVQAALSGTAPFTLTWSDG
                     FVQSGIATTLATRSVSPATTTTYTVTAVRDGSCSGSASGSAAVTVFESPRAVVSTPSV
                     LICLGGSATITAQLSGPGPYTLTWSDGVVETVAGPASTRTVTPYLTETYSVVTVSNGT
                     CTVPGTGSTRVVVFPDASAATITAPAEVCAGSGGHVASVPAAGDGTTWVWTATNGTID
                     SGQGTRSVVFSAGASGDTTLSVTVHAAGGCEVSGSLRVRTAARPPLPAIQAPASLESG
                     EAGAVAEVAGAEGLTCAWTISNGTITDGADTCRITFSAGDPGLVVLGVVTRNAKGCAS
                     APGQVEVPVRGLSSVLTAPIVLDVAGPGGSRYATELTLANPSPSAVKATLLYTPATSL
                     GARGGGTVSETLEGGRQRIVPDLLAWLRTKGLEIPSDGSSQGGTLRVTFENVAAGWSP
                     SASTRTTVASGAGRAGLSMTAVDPAVANASKVWLFGLRENAADRSHVALANLGETGSL
                     DLRVTLHSGEGAAVPKHVLPVVRLEAGQWLQLNSVLHEAGFTAGYALVERIAGSAPFH
                     AYAVFNDNTTHDGSVVPATPAAGLGGTRLVPVVVETGSFTTELVLANPSARAVKARLW
                     FIESLANPGGYSLGGVTVDLAAGEQKVLPNVVSAMRQLGLVVTPAKRDYAGTLAVTFS
                     AAGEPAEGWAGARTATAGAGPGRYGLFCPAPAPDAAPGEAWIFGLIQDASNRSNLALL
                     NAAANLGPVTLRYSVFDGATGRKVATSDPVTLGAGAWMQINGALSKWKVAQGYVKVER
                     IEGTAPFLAYAVVNDGGVPGAGTGDGSYVEMIPIVR"
     gene            7239..7934
                     /locus_tag="P5164_08815"
     CDS             7239..7934
                     /locus_tag="P5164_08815"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRY44032.1"
                     /translation="MAHSAALPAGPKARSSSGPARRSLPAADPEKRKRLLHAAAKTFG
                     RRGFHEAKIADIAAEAGVAEGTVYLYFRNKEDLLGCVFDETMDEVLAEARAVRHAGGG
                     AAEKLVRLVGLHLSFVGQDRDLASVFQIELRRSARLVERFSRSKLVEYFRLLDGVLKE
                     GIASGELRRDLEPRLAVRILFGAADEILSEWLLSGEPKPAADAEKLVGTLLHGFLSCE
                     KSRRAPRHRRTTT"
     gene            7931..9190
                     /locus_tag="P5164_08820"
     CDS             7931..9190
                     /locus_tag="P5164_08820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HRY44033.1"
                     /translation="MSRAAYILDGARTAFTKAGTDFRDVSATDLGRAAAVEAMARSKV
                     EPAEIDQAIFGNIATPPDAANIGRVIALRAGVPKEKPAHSVSRNCASGIESVVQAARL
                     IQTGEADVVLAGGVECMSLIPFLYREDVKAVLVEAGMAKTAGARFGAFRKMPWKQLLN
                     PVIGIKLGLTDPVCGLNMGETAELLAKEGGLTREMQDAFALRSHQRAQAARERLAEEI
                     VPVPLPPKFDKVATLDNGVREGQTMEALAKLKPFFDRTYGSVTAGNSSQITDGAAAMV
                     VASEDFVAKRGLPVLGRIAGWGVAGLEPERMGLGPSRSTPVALKHAGVAWKDVGLMEM
                     NEAFAAQVLANLLAFEKDPSLGPVDPETLNPNGGAIALGHPVGASGSRLILTLLKEMK
                     RRKTRHGIATLCIGGGQGQTIVLEGVA"
     gene            9190..>9561
                     /locus_tag="P5164_08825"
     CDS             9190..>9561
                     /locus_tag="P5164_08825"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRY44034.1"
                     /translation="MADAPGAFRLDVEGDGLAVLTFDLPGEKVNKYSAHVLLELERVL
                     DGLAKRSDVKALLLVSGKPDIFIAGADVKEIAGVSDPATIANGVRRGHEIFGKLANLP
                     FPTVAAIEGPCVGGGCETVLAM"
BASE COUNT         1490 a         3202 c         3526 g         1343 t
ORIGIN      
        1 gtcgtgaccg gcgcgggcgg ccccctcggc gagcgcacgt ccgagccggc gggaagcccc
       61 ggtgacgagg gcgacgggtc tcatggcgcg gagtgtctca ccgcggccgg gcgccgcgcc
      121 gtccctataa tgccgctacg caacacggcc gggccgggga gggtgagatg acgacgccga
      181 gcactctggt cgacgtattc cgcaacctcg gggcgatgcc gaagccggac ctcctcctgt
      241 acaagaagga cgggcgctgg gtcccgatct cgacggcgga gttcaccgac cgcgtgaagg
      301 ccgtttcggc ggcgctgggc accgtcggcg tcccggacgg gggggccgtc gtcctcctct
      361 ccgagaaccg tccggagtgg ccgacgatcg acttcgcctg ccagtgctac ggggccgtcc
      421 tcgtccccgt cttcccgacg atggtggccg accaggccga gttcatcgtc cgcgactgcg
      481 gcgccacggt ggcgttcgcc tcgacgccgg atcaggcggc caagctcctg aaggcccagg
      541 cgacgtgccc gaagctgaag cacgtcttcg tcttcgacca tgccggaggg ggcgggaagc
      601 cgttcggcgc gctcgtcgag gagggccgaa aggcccacgc cgcctcgccg gacctcttcg
      661 acgagcgggc cggacgacgg aaggcgtcgg acctcgccac gatcatctac acctccggga
      721 cgacggggga gccgaagggg gcgatgctca tgcagagcaa cttcgtctcc aacatgctcg
      781 ccggctgctc ccttctgccg ttcgactcga ccgccgtgac gatgtcgttc ctcccgctcg
      841 cgcacgtctt cgagcggctc gtcgagtact gctactacca ccggggcgcg acgatcgcct
      901 tcgccgagtc gatcgacaag ctccgggaca acctcggcga ggtgaacccc cacatcttcg
      961 gcgccgtccc ccgcgtctac gagaaggtct atgcccgcgt catggacaag gtcgacgccg
     1021 gctccccggc gaagcagcgc ctcttccgga ccgccatcgg ggtcgggaag gaggtcctcc
     1081 gtcttcgcac ggagaagaag acccccggcg gcgccctcgc gctccagcac ttcctctacg
     1141 agcgcctcgt cttccggaag atccgggagg cgctcgggac ccggttccgg ttcgcgatct
     1201 cgggcggggc cccgctcgcg aaggacctgg cggagttctt ctgggcgatc ggcgtcgagg
     1261 tctacgaggg ctacgggctc accgagacga gccccgtcat cgccgtgaac tgcccgtcgg
     1321 cctggaagct cggcaccgtg gggagggtca tgccgggagt cgaggtgagg atcgccgacg
     1381 acggcgagat cctcacgcgc ggcccgcacg tgatgaaggg ctactacggc cgccccgagg
     1441 cgacggccga gacgatcgac gccgacggct ggctccacac cggagacatc ggcctgctcg
     1501 acgaggaagg cttcctccgg atcacggacc ggaagaagga gatcatcgtc aactcgaacg
     1561 ggaagaacgt cgctccggcg ccgatcgaga gcgccctgaa gggagacccg ttcgtcgcgc
     1621 agccggtcgt catcggcgac cgccggaagt tcctctcctg cctcatcgtc ccgaacttcg
     1681 agaagctccg cgagtgggcc gcgaagaacg ggctcgggtc cctctccgac gaggagctcg
     1741 tcgcccaccc gcgggtcgag cagatctacc aggggaccgt ggaccgctgg aacgaaggga
     1801 agccgaacga gcagctcatc cgagccttca agctcctccc gaaggagctg acgatcgagg
     1861 gcggggagct gacgccgacg ctcaaggtca agcgccggat cgtcgaccag aagttccgca
     1921 gcgtgatcga cgggctgtac aagggggact gaccgaccgg gacgagagcc gggcccgcca
     1981 gtggggcgga cccggccctc gagagctcat cggacgatgg ggatcatctc cacgtaggaa
     2041 ccgtctccgg tcccggcgcc cgggaccccg ccgtcgttga cgacggcgta ggcgaggaag
     2101 ggcgcggtcc cttcgatccg ctccaccttc acgtagccct gggcgacctt ccacttcgag
     2161 agggcgccgt tgatctgcat ccaggctccg gccccgagcg tcaccgggtc ggacgtcgcg
     2221 accttgcggc cggtcgcgcc gtcgaagacg ctgtagcgga gcgtgaccgg gccgaggttc
     2281 gccgcggcgt tcaggagcgc gaggttcgag cggttcgagg cgtcctggat caggccgaag
     2341 atccacgcct cgcccggcgc ggcgtccggc gccggcgcgg ggcagaagag accgtagcgg
     2401 ccgggtccgg cgccggcggt cgccgtgcgc gcccccgccc agccctccgc cggctcgccg
     2461 gcggccgaga acgtcacggc gagcgtgccg gcgtagtccc gcttcgcggg cgtgacgacg
     2521 aggccgagct gccgcatcgc ggagacgacg ttcgggagga ccttctgttc gccggcagcc
     2581 aggtcgaccg tgacgccgcc gagggagtag ccgcccgggt tcgcgagcga ctcgatgaac
     2641 cagagccggg ccttgaccgc acgcgcggac gggttcgcga ggacgagctc ggtcgtaaag
     2701 gagcccgtct cgacgacgac cgggaccagc cgggtccctc cgagacccgc ggccggagtc
     2761 gccgggacga cggagccgtc gtgggtcgtg ttgtcgttga agacggcgta agcgtggaac
     2821 ggggcgctgc cggcgatccg ctcgacgagg gcgtagcccg ccgtgaaacc cgcctcgtgg
     2881 aggacggagt tcagctggag ccactggccg gcctcgaggc ggacgacggg gaggacgtgc
     2941 ttgggaaccg cagccccctc gcccgagtga agagtgaccc ggaggtcgag gcttcccgtc
     3001 tcgccgaggt tcgcgagcgc gacgtgcgat cggtcggcgg cgttctcccg gaggccgaac
     3061 agccagacct tcgatgcgtt cgcgacggcg gggtcgaccg ccgtcatgga aaggccggcg
     3121 cggcccgcgc cggaggcgac ggtcgtccgg gtcgaggccg aaggggacca cccggcggcg
     3181 acgttctcga acgtcacgcg gagcgtgccg ccctgcgaag agccgtcgga cgggatctcg
     3241 agccccttcg tccggagcca ggcgaggagg tccggaacga tccgctggcg gccgccctcg
     3301 agcgtctcgg agacggtgcc gcctccccgg gccccgaggg acgtggcggg cgtgtagagg
     3361 agggtggcct tcacggcgga gggcgagggg ttcgcgagcg tgagctcggt cgcgtagcgg
     3421 ctcccgccgg ggccggcgac gtcgaggacg atcggcgcgg tgaggacgga gctgaggccg
     3481 cgtacgggga cctcgacctg cccgggggcc gaggcgcacc ccttcgcgtt ccgcgtgacg
     3541 acgccgagga cgacgagccc cggatccccc gcggagaacg tgatgcggca ggtgtcggcg
     3601 ccgtccgtga tcgtgccgtt ggagatcgtc caggcgcagg tcagcccctc ggctccggcg
     3661 acctcggcga cggcgccggc ctcgccgctc tcgaggctcg cgggggcctg gatggcgggg
     3721 agcggaggcc gcgcggccgt ccggacgcga agggagcccg agacctcgca gccgccggcg
     3781 gcgtgcacgg ttacggaaag ggtcgtgtcg ccggaggcgc ccgcggagaa gacgaccgac
     3841 cgggtgccct ggccggagtc gatcgtgccg ttcgtggccg tccacaccca cgtggttccg
     3901 tcgccggcgg cggggaccga cgcgacgtgg ccgcccgagc cggcgcagac ctcggcgggc
     3961 gccgtgatcg tggcggcgga ggcgtcaggg aagacgacga cccgggtcga tccggtgccg
     4021 ggcaccgtgc aagtcccgtt cgagacggtc acgaccgaat aggtctcggt caggtacggc
     4081 gtgacggtcc gcgtcgaagc cgggcccgcg accgtctcga cgactccgtc gctccaggtc
     4141 agcgtgtagg ggcccgggcc ggagagctgc gccgtgatcg tcgcgctgcc tccgaggcag
     4201 atgaggacgc tcggcgtcga gacgacggcc ctcggcgact cgaagaccgt gaccgcggcg
     4261 ctcccggagg cggagccgga gcagctcccg tcccggaccg cggtcacggt gtaggtcgtg
     4321 gtagtcgcgg ggctcacgct gcgcgtcgcg agcgtcgtcg cgatgccgga ctggacgaac
     4381 ccgtcggacc aggtcagggt gaacggtgcc gtccccgaaa gcgccgcctg gacggtcgcc
     4441 gcgttgccgg cgcagatcgt gcccccgccg gaaacggtcg cggacggggc gggggcgagg
     4501 gaggaaacgc gggtggagct ggccctgcag ccgctcggga gcgtgaccgt gacgccgagg
     4561 gtgacggcgg ctcccgtcgg ggtgaacgtc acggaccgtc cggaggtgct ccccgcgatc
     4621 gtcccgccgg cgattgtcga ctcgtaggtc gccccgcccc ctgcatcggg aaccgaggcc
     4681 gcaacgtcag cggcgcccgc gcagtaggag cccggggcgc tgatcgaagc ggagggaacg
     4741 gggttgacga tgaccggagc gctccccgaa gccctgccgc cggcgcagcc gtaggcgtcc
     4801 gtaaggctcg aaatggcgta aatcgtgctc gtcgcggggg agacggtccg ggtctcaggc
     4861 ccggcggcgc cgacggactg gaccactcca tcggaccacc tcaccgtgaa gggcggcacg
     4921 ccgtggagga cgaacgagac gagggccgtc tcgccggcgc agaccgacca gccgctggtg
     4981 acggtggcgg acgggggaag ggagacgggg aggtcgcggg aggccgtcgc gctgcacgag
     5041 ccgatcgtcg cgatgacgga gacagtcacg gggttcgtcg ttccgacgaa agacaagacg
     5101 ttcgtcccct gtccggagac gatcgttccg ttcgtgacgg tccagttgta gagggcgccc
     5161 gtcgtcgcgg ggatggaggc cgtcgcgttc gttcccacgc agacgcgggc tggcgccgtg
     5221 agcgtcgcat tcgatgtgga ccagaccgtg atggtggcgg aaccgctcgc cgtgccggcg
     5281 cagaggccgg cggtcaccgt acgaagcgtg taggtcgtcg tcgcgcccgg agtgacgtcg
     5341 cgggtcgccg ggctcgtcgc gattccgttc tgaaagaagc cgtccgacca gacgaggttc
     5401 cacggtcccg accccgtgag ggcggcggag agcgtcgcgc tctcaccgat gcacacggtg
     5461 ccgctgccgc tgacgctggc tgtcggcgcg gtgccgccgc agacgtaggt gaagccgttc
     5521 cggattcgac cgacgtcgcc gtcggggttc tcgacctcga cgtctacgag tccgttgacg
     5581 tgggggccgg taatggcatt gatcagattg gatgcgacgc tcgtgacgac ggccgggatc
     5641 ccgccgatgc ggacgacggc cccggtctgg aagcctgagc cggagatcgc gaccggcgtt
     5701 ccgccgaggg aggggcccga ggtgggagag atggacaccg gcttcgcgct cgacgtcagc
     5761 gagatcgcgg tgagggtacc gtcgtgcgcg gcgacgaaga gtctcccgtc cgcgatcgcg
     5821 agctccccgt agggagccgc gaacgtccag acggcctcgt gagtcgtcag gtcgatcgcg
     5881 tagacggtgt cacgggtcga gacgaggaca tgcgtgtcgg tcacgatcat cggttgggta
     5941 attgcctgtt tgggctccgc cggaggaggg gtccaggacc aaacgagggt gtgggaggac
     6001 tcgtcgtaga cggacagccc gccattgttc gcggcgtata tccgcccctt cgcgacactc
     6061 ggctggccgg tgaagccctc gctcagctgc cagccgatcg ttccggccat cgtgttgaag
     6121 ctgatgaggc gaccgttgtg gatcgcgagc accccgtacg cgccgagcac cggcgcgagg
     6181 ttcatgctcc agtcgtccca ctcgaaatcc ggatcgggga tgaagcctgg cgttgaggcc
     6241 gtgccatcca ggagattctt gatgtagagc cccggggcgt agctgcccac gtaggcgtag
     6301 gcgctcgtcc cgtcgacggc cggcgtccac tcgtggtatt gcgggaggtc cgcataccag
     6361 aactgatcac cgttcaacgc gctgaacgcg tacatgccac cgtactctcc gccgttgacg
     6421 tagaccctgc cgttggagac cgtcggcgag aagtacctct cccactgcgc cccgtgtgga
     6481 gactgaaaga tcgtcgtgcc ggaagacccg tcaaaggccc agagccaggt atcggaggag
     6541 ttgcccgttt gcacgtagac ccggccgttc acgtagctcg gggggttgac cgagaagaca
     6601 gctccgaacc ctttggtcca aagcgtcgcg ccatcgctcg tacggagcgc gaagagctgc
     6661 gggacgtcgt tgaaacgtgt caggagtgtc gcgtagaccc ttccgtcccc cgccgccaca
     6721 gggttgacgg ggagagtgaa gttcggaatc gtcccgaggt ttctcgtcca tcgaacgtgg
     6781 aaccactgcg gctcgagcga caccgggacg tagcccgtgt gcgcggggtt cccctggaac
     6841 atcggccagc tctgggccgc agcggggcgt ggatggaaca gccagacggc caggacgaga
     6901 aacgttggga ggacgaggcg ggaggaggac cggagagcgg tggacatacg cacgcgaaga
     6961 agcctcctcg agtctgagtc ggggcgggta agaatgatcg gaaaccgaac aggaggtcgg
     7021 ttttggtggc cttcgtcgcc gaagacatac cgggatccgg cgcgtttcgg caatcggaag
     7081 gccatcgagg gattgtacga tcgatgtcgc tgcgctggcg aatcggaaac cgttctcaag
     7141 tgcaccagcg gtgggtgact gtgcccggac tgggagcagc gggtcgcgcg gccggggcat
     7201 ccttgactcc cgccacgccg caacataggc tctgagtgat ggctcattca gccgctctcc
     7261 cggcgggtcc gaaggcccgc tcctcctccg gtccggctcg gaggagcctc ccggccgccg
     7321 accccgagaa gcgaaaacgg ctcctgcacg ccgcggcgaa gacgttcgga cgccgcggat
     7381 tccacgaggc gaagatcgcc gacatcgcgg cggaggcggg cgtcgccgaa gggaccgtct
     7441 acctctactt ccgcaacaag gaagacctcc tcggctgcgt cttcgacgag acgatggacg
     7501 aggtcctcgc cgaggcgcgg gcggtccgcc acgcgggtgg gggcgccgcc gagaagctcg
     7561 tccgcctcgt cggcctccac ctgagcttcg tcgggcagga ccgcgacctc gcctccgtct
     7621 tccagatcga gctgaggcgg agcgcgcgcc tcgtcgagcg cttctcccgg agcaagctcg
     7681 tcgagtactt ccggctcctc gacggcgtcc tgaaggaagg gatcgcctcg ggggagctcc
     7741 ggcgggacct ggagcctcgc ctcgccgtcc ggatcctctt cggggcggcg gacgagatcc
     7801 tctcggaatg gctgctgtcg ggagagccga agcccgcagc cgacgcggag aagctcgtcg
     7861 gcacgctgct gcacgggttc ctgtcctgcg agaagagccg gcgggccccc cgccacagga
     7921 ggacgacgac ttgagccgag ccgcctacat cctcgacggt gcgcggaccg ccttcacgaa
     7981 ggccgggacc gacttccggg acgtctccgc gaccgacctc ggccgcgccg ccgccgtcga
     8041 ggcgatggcc cgttcgaaag tggagcccgc cgagatcgac caggcgatct tcgggaacat
     8101 cgcgacgcct cccgacgccg cgaacatcgg gcgcgtcatc gccctccggg ccggagtccc
     8161 gaaggagaag ccggcccact ccgtctcgcg caactgcgcc tcggggatcg agtcggtcgt
     8221 ccaggcggcg cggctcatcc agacggggga ggccgacgtc gtcctcgcgg gcggcgtcga
     8281 gtgcatgtcg ctcatcccgt tcctctaccg cgaggacgtg aaggccgtcc tcgtggaggc
     8341 cggaatggcg aagacggccg gggcccggtt cggcgcgttc cggaagatgc cgtggaagca
     8401 gctcctgaac ccggtcatcg ggatcaagct cggcctgacc gaccccgtct gcggcctgaa
     8461 catgggcgag acggcggagc tcctcgcgaa ggaaggcggg ctcacccgag agatgcagga
     8521 cgccttcgcg ctccgctcgc accagcgggc gcaggcggcg cgcgagcgac tcgccgagga
     8581 gatcgtcccg gtcccgctcc cgccgaagtt cgacaaggtc gcgacgctcg acaacggcgt
     8641 ccgcgagggg cagacgatgg aggccctcgc gaagctgaag ccgttcttcg accggacgta
     8701 cggctcggtg acggcgggga actcctcgca gatcaccgac ggggcggccg cgatggtcgt
     8761 cgcctcggag gatttcgtcg cgaagcgggg cctccccgtc ctcggccgga tcgcggggtg
     8821 gggcgtcgcc gggctcgagc cggagcggat gggcctcggg ccgtcgcgct cgacgcccgt
     8881 cgccctgaag cacgcgggcg tcgcctggaa ggacgtcggt ctgatggaga tgaacgaggc
     8941 gttcgcggcg caggtcctcg cgaacctgct cgccttcgag aaggatccct cgctcggccc
     9001 cgtcgacccg gagacgctca acccgaacgg cggggcgatc gccctcgggc accccgtcgg
     9061 cgcctcgggc tcgcgcctca tcctgaccct gctgaaggag atgaagcgtc ggaagacgcg
     9121 ccacggcatc gcgacgctct gcatcggcgg cgggcagggg cagacgatcg tcctcgaggg
     9181 ggtggcgtga tggccgacgc tccgggcgct ttccgcctcg acgtcgaggg cgacggcctc
     9241 gccgtcctga cgttcgacct gccgggcgag aaggtgaaca agtactccgc ccacgtcctc
     9301 ctcgagctcg agcgggtcct cgacggcctc gcgaagagga gcgacgtgaa ggcgctcctc
     9361 ctcgtctcgg ggaagcccga catcttcatc gccggggccg acgtgaagga gatcgccggg
     9421 gtcagcgacc cggcgacgat cgccaacggc gtgcggcgcg ggcacgagat cttcggcaag
     9481 ctcgcgaacc tgcccttccc gacggtcgcg gccatcgagg ggccgtgcgt cggcggaggc
     9541 tgcgagacgg tcctcgcgat g
//
LOCUS       DAOTEZ010000138         9554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5057_length_9554_cov_6.290346, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000138 DAOTEZ010000000
VERSION     DAOTEZ010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9554
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5057_length_9554_cov_6.290346"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..465
                     /locus_tag="P5164_08830"
     CDS             115..465
                     /locus_tag="P5164_08830"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRY44035.1"
                     /translation="MDVRETPAWRKAIGLAHEVCATLEGAAELPGDSGQRLRRAALAV
                     PSLVADAFLDTGPADCRERLSAAAARVAELRQLLRAPGIRKFVPGPEADALLAEAESL
                     ALEIRALGGRCPEA"
     gene            525..863
                     /locus_tag="P5164_08835"
     CDS             525..863
                     /locus_tag="P5164_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44036.1"
                     /translation="MTFRPLAAILAALVLLTAPCALAQDAAPATTEAAPAVTEAAPAT
                     TETAPAVAPATTTEAAPAGHEGDHAAAPAPAATAEVPVGTSQTTTIVIGLIVLLLLIV
                     LVILGTKKQE"
     gene            complement(1583..3766)
                     /locus_tag="P5164_08840"
     CDS             complement(1583..3766)
                     /locus_tag="P5164_08840"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRY44037.1"
                     /translation="MGNELFLGRSVTKAPAPLTLDPDDLTTHGLIVGMTGSGKTALGI
                     LLIEELLKQGIPVLAIDPKGDLGNLALAFPRLAPDEFAPWVPAGGEATPEGEAKKWAD
                     GLAGWGISVADVASYAAGRTVTIYTPGSTAGVQVDLLGALAPPPDGPQSEEGRDLMTA
                     FLGGLLGLLGRNADPVSSREFILLARCVEEIWGKGETATLEALVGAAAQPPFSAIGAL
                     PLETFFPKKERDGLVLALNGLLASPATAAFRGGAPLDVDVLLGGKGSPARCSIFSIAH
                     LSDPERLFVVATLLSRVRSWLRSQPGSTTLRALVYIDEIFGFFPPSGEPPTKKPLLAL
                     LKQARAFGVGTVLATQNPVDLDYKGLANCGCWWVGTLQTERDRRRLADGLTDAAGLTD
                     VDALLNQTKKRTFLLHDVHRKGPEIFETRWAMSYLRGPMTRDELTKLKKTAPAAAGGV
                     AAAAAPTVAAKPSAPAGPPMLPAEWPARWLDRRGGERAVPYLWAKAAVRYKSGKGTSD
                     EITSVKLFPLEADTPREVVQGDALDLEGKELLSSAPRTLSYGELPSWVGAKGVKEAEK
                     ALKERLPDLLATTLLRDPLTGLLSNPGEDEDAFAARVGEKAKPSAALVERIEKKKRDL
                     AAAEQAEKARSMETMATMAGAAMDVLGGLFGKKKSLRVSKVGSVLSKRRMEGAAETKI
                     ETLKAEIEELESKIAAPDPARFEKVDVVPTKTGVDLLGVGVAWVS"
     gene            3836..4225
                     /locus_tag="P5164_08845"
     CDS             3836..4225
                     /locus_tag="P5164_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HRY44038.1"
                     /translation="MNEGRSPAIRVTLMPKDTNVHGTIFGGVLLSHLDLAGAVAARRH
                     CDRMVTVKMTEVVFHEPVFVGDLVSFYADVLRIGNTSITVKVTVEASRHWGAGEATKV
                     TEAEIVYVNVDENRRPVPITRREIAAG"
     gene            complement(4229..4660)
                     /gene="arfB"
                     /locus_tag="P5164_08850"
     CDS             complement(4229..4660)
                     /gene="arfB"
                     /locus_tag="P5164_08850"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HRY44039.1"
                     /translation="MPDPIDVVPGVRIPAAALEARGVRSGGPGGQNVNKVASKAELFV
                     DLALVEGLDDGARARLLALAEGRRDADGFLRLTAGESRDFHRNLERAREKARALVAKA
                     LVVPRKRRATRPTAGAREARLALKKRLAKLKRNRARPRGED"
     gene            4704..6194
                     /locus_tag="P5164_08855"
     CDS             4704..6194
                     /locus_tag="P5164_08855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HRY44040.1"
                     /translation="MPAFSSARIFDARRRAIDVLLAALVVLGATGLGRLGGANPTTAG
                     FLFLLAVLGLAVARGLTASVAASVGATLAFNWFFLPPLHTFTIAAPANWVALFSFLVV
                     AVVVSRLVLRAREEADDALRRRREIEVLYDLSIDLFTATNRVGVLGEAAARALRSVGA
                     EGGGLLEVDEGSDRPRVVSWSGRDLAPEHERLVKVVLDRAEPAEVPAPGGASDAYFPL
                     VVGGKARGVLVVLGARAGRTILEPVARLVALAVERERFLAENAHLEALRQSEGLKTAL
                     LRAVSHDLRSPLTAIGLSIEGLRRRLSADADARPALDDLSRESSRLSRRIDNLLALAR
                     LEAGRSVPHPEPTPPADLFRAARESLPLVLDAHPVHVSVASDAPDVLADPSLVAEVLV
                     NLVENAARAAPEGTVIELTATRHPIEPLLVRLEIADRGPGLPRETLPGAEAEPADGGR
                     RGLGLEIARGLAVANGGGLSLHPRPGGGTIARVDLPAAPAAEEPAP"
     gene            6191..6892
                     /locus_tag="P5164_08860"
     CDS             6191..6892
                     /locus_tag="P5164_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY44041.1"
                     /translation="MTHGGACVLVVEDDPTIRRGLAAELSANGYDVLEAADGRRGIAL
                     FASGAPDLVLTDLAMPVADGFELIREVRRTGATPVVVLSVRGADVDKIRALDLGADDF
                     VTKPFSVPELLARIRAQLRRSGVAPARTVLEFEGLSIDLDRRRVVQGEREVKLTPTEF
                     AILELLATNAGRPVTTAKIIARVWKGAPGTTVDVVRVHVGTMRKKLEPDPASPRYVVT
                     EPWVGYRFVAEPLPD"
     gene            complement(6899..8887)
                     /locus_tag="P5164_08865"
     CDS             complement(6899..8887)
                     /locus_tag="P5164_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006919160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HRY44042.1"
                     /translation="MPILESVRRFVIGKERDPLDPKIFHRVSLAAFLAWVGLGADGLS
                     SSCYGPEEAFVTLGAHSHLAVFLAIAMVITIGVISASYSQIISLFPSGGGGYLVATIL
                     LGRGAGVVSGSALVVDYAMTIAMQVAACVAALCSLAPRAVPQREQLVVSLVLLVALVV
                     LNLRGVKESVLVLLPIFILFLVTHALLIAYGIGSHVGAMPEVVAATARETRETWTSLG
                     AIGTLALLLRAFSMGGGTYTGIEAVSNGLQILREPRVRTGRRTMVYMGTSLALTAGGL
                     ILCYLLAGVHRVPGRTLNAVLTERLATEAFGPGSLAGAFLLATLVAEGALLLVGGQAG
                     FLDGPRVLSNMAIDSWVPHRFSLLSDRLVTQNGILVMGGAAFGILLYTGGSIDVIVVM
                     YSINVFLTFSLSQLGMCVHWWKVRREDPLWKRHLSINGFGLMLTATLLVATVAMKFRA
                     GGWVTVLITGSLVVVCVLVRRHYSSIRLYLKRTDDVLTTLKPPKPADSGELPKIEPPK
                     RGTPTAVFLVSGFNGLGMHSFLNVQRYFPGYFRHCVFLSVGVVDSASFKGAKEIENLR
                     RETERDLVRYVAFARGNGLAAEHHLSVGTDLLDELVDLCRKVRDDWDRPIFFMSKLVF
                     PKENALNRLLHNQTPFALQRRLQMEGMHSVIMPIQTGV"
     gene            9312..9401
                     /locus_tag="P5164_08870"
     CDS             9312..9401
                     /locus_tag="P5164_08870"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0043462 - regulation of ATP-dependent
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit F"
                     /protein_id="HRY44043.1"
                     /translation="MSAESLAGLVLSGLALAYLLFALLKPEKL"
     gene            9403..>9554
                     /locus_tag="P5164_08875"
     CDS             9403..>9554
                     /locus_tag="P5164_08875"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HRY44044.1"
                     /translation="MTALAFLKIAVYFLLVLAVTKPLGLYMRRVFSGERTFLDPVLLP
                     VERALYR"
BASE COUNT         1297 a         3539 c         3380 g         1338 t
ORIGIN      
        1 caccgggtct gtactgatcc gaccgggaag cgtagcaggg gggggaatcc cccgaaaggg
       61 gaaccgaagg cggaacgccg caggcccctc gtccgtatat gcttcggacg aacgatggac
      121 gtgcgcgaaa ccccggcctg gagaaaggcc atcggccttg cgcacgaagt ctgcgcgacg
      181 ctcgagggcg cggcggagct ccccggcgac tccgggcagc ggctgcggcg cgccgccctg
      241 gccgtgccgt ccctcgtcgc cgacgcgttc ctcgacaccg gccccgccga ctgccgggag
      301 cggctgagcg cggccgcggc gcgggtcgcc gagcttcgtc agctccttcg ggcgcccggg
      361 atccggaagt tcgtccccgg gccggaggcc gacgccctcc tggccgaggc ggagtcgctg
      421 gcgctggaga tccgcgcgct cggcggccgc tgccccgagg cctgaccctt cccgatttcg
      481 cctcctcctg ctagcgttcc cccgctggaa ggaaaggagt cccgatgacg ttccgcccgc
      541 tcgccgcgat cctggcggcc ctcgtcctcc tcacggcccc ctgcgccctc gcccaggacg
      601 ccgcccccgc gaccacggag gccgcaccgg ccgtgaccga ggccgccccg gcgacgacgg
      661 agacggctcc cgccgtggct ccggcgacga cgaccgaggc cgcgcccgcc ggccacgagg
      721 gcgaccacgc cgccgccccg gcccccgccg cgacggcgga ggtccccgtg ggcacctccc
      781 agacgacgac gatcgtcatc ggcctcatcg tcctcctcct cctgatcgtc ctcgtcatcc
      841 tcggtacgaa gaagcaggag tagggtcgac cgggaatccc gggaacagac cagacgcggg
      901 gcaccggggc accggagagg ggagcctcac cccgggggta ggtcccacgg cctccggctg
      961 cgcgcggccg acccgcaggg cgtgcggcgt tcggcgctcg ggtcgctcgc gaactcgctc
     1021 gcctgcggct cgctcaaaca gcgctcgctt tcccccgagc gcctcgcggc gcgcgccagc
     1081 gggtccccgg ccgtgctcga gtcggctcgc gggacctacc cccggggctc ggccgcccgg
     1141 agcaagcacc cgccaggatg ggggtctgac ccccactcta tgttctacgg tgaggagctt
     1201 gcgctgtgtt cggccccacc cttccccctg agagcgccgg acttgccacg gccgcgggag
     1261 gggcggggcg tctcggagac gaccccgagc acggccgggg acccgctcga cgtcgcgagg
     1321 accgggcggg gaggaggccc gcgcgctgtt cgacgaccga agggaggagt tcgcgcgggc
     1381 gccccgctcg gccgcagcga ggtcgtcagc gggtcggccg cgcgcagaag gagtcggaga
     1441 gacgctccgc ccctcccgcg gccccctgcg gaatcgacga gagcctgcga ctggtaggtg
     1501 ctctggttcc ccggttcccc ggttccccgg ttccccggtt ccccggttct cccgtgctcc
     1561 cggcgctccg gtctcccccc ggtcagctca cccaggcgac gccgacgccg aggaggtcga
     1621 ctcccgtctt cgtcgggacg acgtcgacct tctcgaagcg ggccgggtcg ggggcggcga
     1681 tcttcgactc cagctcctcg atctccgcct tgagggtctc gatcttcgtc tcggcggcgc
     1741 cttccatgcg gcgcttgctg aggaccgagc cgaccttcga gacgcggagc gacttcttct
     1801 tgccgaagag gccgccgagg acgtccatcg ccgcgccggc catcgtggcc atcgtctcca
     1861 tcgagcgcgc cttctcggcc tgctcggcgg ccgcgaggtc acgcttcttc ttctcgatcc
     1921 gttcgacgag cgcagccgag ggcttcgcct tctcgccgac gcgggcggcg aacgcgtcct
     1981 cgtcctcgcc cgggttcgag aggaggccgg tgagggggtc gcggaggagc gtcgtggcga
     2041 ggaggtcggg gaggcgctcc ttcaacgcct tctcggcctc cttcaccccc ttcgcgccga
     2101 cccacgaggg gagctcgccg taggagagcg tgcgcggcgc cgaggagaga agctccttgc
     2161 cttcgaggtc gagcgcgtcc ccctggacga cctcgcgcgg cgtatcggcc tcgagcggga
     2221 agagcttcac ggacgtgatc tcgtccgagg tccccttgcc gctcttgtag cggacggcgg
     2281 ccttcgccca caggtacggc acggcgcgct cgccgccgcg ccggtcgagc cagcgggccg
     2341 gccactcggc cgggagcatc ggcgggccgg cgggggccga gggcttcgcc gcgacggtcg
     2401 gcgccgcggc ggccgcgacg cctccggccg ccgccggcgc cgtcttcttc agcttcgtca
     2461 gctcgtcgcg cgtcatcggg ccgcggaggt agctcatcgc ccagcgcgtc tcgaagatct
     2521 ccgggccctt gcggtggacg tcgtggagga ggaaggtcct ctttttcgtc tggttcagga
     2581 gggcgtcgac gtccgtgagg ccggcggcgt cggtgaggcc gtcagcgagg cggcggcggt
     2641 cgcgctcggt ctgaagcgtc ccgacccacc agcagccgca gttcgcgagc cccttgtagt
     2701 cgaggtcgac cgggttctgc gtcgcgagga ccgtcccgac tccgaacgcc cgcgcctgct
     2761 tgaggagcgc gaggaggggc ttcttcgtcg gcggctctcc cgacggcggg aagaagccga
     2821 agatctcgtc gatgtagacg agggcccgga gcgtcgtcga gccgggctgg gaacggagcc
     2881 aggagcggac gcgggagagg agcgtcgcga cgacgaagag ccgctcgggg tcggagaggt
     2941 gcgcgatcga gaagatcgag cacctcgccg ggcttccctt cccgccgagg aggacgtcga
     3001 cgtcgagcgg cgcgccgccg cggaaggcgg ccgtcgcagg cgaggcgagg aggccgttca
     3061 gcgcgaggac gaggccgtcg cgctccttct tcgggaagaa cgtctcgagc gggagcgcgc
     3121 cgatcgccga gaaggggggc tgggcggccg cgccgacgag cgcctcgagc gtcgcggtct
     3181 cccccttccc ccagatctcc tcgacgcagc gggcgaggag gatgaactcg cgcgaggaga
     3241 ccgggtcggc gttccggccg aggaggccga ggagcccccc gaggaaggcc gtcatcaggt
     3301 cgcggccctc ttcgctctgc gggccgtcgg gcggcggtgc gagggcgccg aggagatcga
     3361 cctgcacgcc cgccgtcgag ccgggcgtgt agatcgtcac ggtgcggcct gcggcgtagg
     3421 aggcgacgtc ggcgacggag attccccaac cggcgaggcc gtcggcccac ttcttcgcct
     3481 ccccttccgg cgtcgcctcc cctccggccg ggacccaggg cgcgaactcg tcgggcgcga
     3541 gacgcgggaa ggccagcgcg aggttcccga ggtccccctt cgggtcgatc gcgaggacgg
     3601 ggatcccctg cttgagcagc tcctcgatca ggaggatgcc gagggccgtc ttcccgctcc
     3661 cggtcatccc gacgatcagg ccgtgcgtcg tcaggtcgtc cgggtcgagg gtgagcggcg
     3721 cgggcgcctt cgtgacggat cggccgagga agagctcgtt gcccatgaag cgacctcccg
     3781 tgtggagtcc cgtggcgaac gaggatctta ccggccgcgc ggacataatc ggggcatgaa
     3841 cgaaggacgg agccccgcga tccgggtcac gctgatgccg aaggacacga acgtccacgg
     3901 gacgatcttc ggcggcgtcc tcctctcgca cctcgacctc gccggggccg tcgccgcgcg
     3961 ccgccactgc gaccggatgg tgaccgtgaa gatgaccgag gtcgtcttcc acgagccggt
     4021 cttcgtcggc gacctcgtgt cgttctacgc ggacgtcctc cggatcggca acacctcgat
     4081 caccgtaaag gtgaccgtcg aggcgtcgcg gcactggggg gccggggagg cgacgaaggt
     4141 gacggaggcc gagatcgtct acgtgaacgt cgacgagaac cgccgccccg tcccgatcac
     4201 gcggcgcgag atcgccgccg ggtaggccct aatcctcgcc gcgcggccgc gcccggttcc
     4261 gcttcagctt cgcgaggcgc ttcttgagcg cgaggcgggc ctcgcgcgcg ccggccgtcg
     4321 ggcgcgtcgc gcgcctcttc ctcgggacga cgagcgcctt cgcgacgagc gcccgcgcct
     4381 tctcacgcgc ccgctcgagg ttccggtgga agtcccggct ctccccggcc gtcaggcgca
     4441 gaaagccgtc cgcgtcgcgc cgcccctcgg cgagcgcgag gagccgcgcg cgcgccccgt
     4501 cgtcgagccc ctcgacgagc gcgagatcga cgaagagctc cgccttcgag gcgaccttgt
     4561 tcacgttctg cccgcccggc ccgcccgagc ggacgcctcg cgcctcgagc gcggcggcgg
     4621 ggatcctcac cccggggacg acgtcgatcg ggtcgggcac ggcgacatcg tgccacggct
     4681 cctatgatgg agacgagacg cccgtgcccg ccttctcctc cgcccgcatc ttcgacgccc
     4741 gtcgacgggc gatcgacgtc ctcctcgcgg ccctcgtcgt cctcggcgcg accgggctcg
     4801 gccggctcgg cggcgcgaac ccgacgacgg ccgggttcct cttcctcctc gccgtcctcg
     4861 gcctcgccgt cgcgcgcggg ctcaccgcgt cggtcgcggc gtcggtcggc gcgacgctcg
     4921 ccttcaactg gttcttcctt ccgcccctcc acaccttcac gatcgccgcg ccggcgaact
     4981 gggtcgcgct cttcagcttc ctcgtcgtcg ccgtcgtcgt cagccgcctc gtccttcggg
     5041 cccgcgagga ggcagacgac gctcttcgaa ggcggcgcga gatcgaggtc ctctacgacc
     5101 tctcgatcga cctcttcacc gccacgaacc gcgtcggcgt cctcggagag gccgccgccc
     5161 gggcgcttcg atccgtgggg gccgagggtg gcgggctcct cgaggtcgac gagggctcgg
     5221 accggcccag ggtcgtctcg tggtcgggcc gggacctcgc gccggagcac gagcggctcg
     5281 tcaaggtcgt cctcgaccgg gccgagccgg ccgaggtgcc cgcgcccgga ggggcctccg
     5341 acgcgtactt cccgctcgtc gtcggcggga aggcccgcgg ggttctcgtc gtcctcggcg
     5401 cccgggcggg gcggacgatc ctcgagccgg tcgcccggct cgtcgccctc gccgtcgagc
     5461 gggagcgctt cctcgccgag aacgcgcacc tcgaggcgct gcgccagagc gaggggctga
     5521 agaccgcgct cctgcgggcc gtctctcacg acctccgaag cccgctgacc gcgatcggcc
     5581 tctcgatcga gggcctccgg aggcggctct ctgccgatgc cgacgcccgg cccgcgctcg
     5641 acgacctctc ccgcgagagc agccgcctct cgcgacgcat cgacaacctc ctcgcgctcg
     5701 cgcggctcga ggcggggcgg agcgtccctc atccggagcc gacgccgccc gccgacctct
     5761 tccgggcggc gcgcgagagc ctccctctcg tcctcgacgc gcacccggtc cacgtctccg
     5821 tcgcctccga cgcacccgac gtcctcgcgg acccctcgct cgtcgccgag gtcctcgtca
     5881 acctcgtcga gaacgctgcc cgcgccgccc ccgaggggac ggtgatcgag ctgacggcga
     5941 cccggcaccc gatcgaaccg ctcctcgtga ggctcgagat cgccgatcgc ggtcccggcc
     6001 tcccgcggga gacgcttcca ggagcggaag cggagcccgc cgacggcgga cggcgcggcc
     6061 tcggcctcga gatcgcgcgc gggctcgccg tggcgaacgg cggcggcctc tcgttgcatc
     6121 cacggcccgg cggcgggacg atcgcgcggg tggaccttcc cgccgcgccg gccgccgagg
     6181 agcccgcacc gtgacgcacg ggggcgcctg cgtcctcgtc gtcgaggacg acccgacgat
     6241 ccggcgaggc ctcgccgcgg agctctcggc gaacggctac gacgtgctcg aggcggccga
     6301 cggccgcagg gggatcgccc tcttcgcgag cggcgcgccc gacctcgtcc tcacggacct
     6361 cgccatgccg gtcgccgacg gtttcgagct gatccgggag gtgcggcgca cgggcgccac
     6421 tccggtcgtc gtcctctccg tccggggcgc ggacgtcgac aagatccgcg ccctcgacct
     6481 cggcgccgac gacttcgtca cgaagccgtt ctccgtcccc gagctcctcg cgcggatccg
     6541 ggcccagctc cggaggagcg gcgtcgcccc cgcccggacc gtcctcgagt tcgaagggct
     6601 ctcgatcgac ctcgaccggc ggcgcgtcgt ccagggggag cgcgaggtga agctgacgcc
     6661 gaccgagttc gcgatcctcg agctcctcgc gacgaacgcc ggccgccccg tcacgacggc
     6721 gaagatcatc gcgcgcgtct ggaagggggc tccggggacg acggtcgacg tcgtccgcgt
     6781 ccacgtcggg acgatgcgga agaagctcga gcccgacccc gcctcgcccc gctacgtcgt
     6841 cacggagccg tgggtcggct accgcttcgt cgccgagccg ctcccggact gacgcgcctc
     6901 agacccccgt ctggatcggc atgatgacgg agtgcatccc ctccatctgg aggcggcgct
     6961 gcagcgcgaa gggcgtctgg ttgtggagga ggcggttcag cgcgttctcc ttcgggaaga
     7021 cgagcttgga catgaagaag atcggccggt cccagtcgtc cctcaccttc cggcagaggt
     7081 ccacgagctc gtcgaggagg tcggtcccga ccgagaggtg gtgctcggct gcgagcccgt
     7141 tcccccgggc gaacgccacg taacggacga ggtcacgctc ggtctcccgc cgcaggttct
     7201 cgatctcctt cgcccccttg aagctcgccg agtcgacgac tccgaccgac aggaagacgc
     7261 agtgccggaa gtagcccggg aagtaccgct gcacgttcag gaacgagtgc atcccgaggc
     7321 cgttgaaccc cgagacgagg aagacggcgg tcggagtccc acgcttcggc ggctcgatct
     7381 tcgggagctc ccccgagtcg gccggcttcg gaggcttcag cgtcgtcagg acgtcgtccg
     7441 tccgcttcag gtagaggcgg atcgagctgt agtggcgccg cacgaggacg cagacgacga
     7501 cgagcgagcc ggtgatgagg accgtcaccc accccccggc gcggaacttc atcgccaccg
     7561 tcgcgacgag gagcgtcgcc gtgagcatca ggccgaagcc gttgatcgag aggtgtcgct
     7621 tccagagagg gtcctcgcgc cggaccttcc accagtggac gcacatgccg agctgcgaga
     7681 ggctgaacgt gaggaagacg ttgatcgagt acatgacgac gatgacgtcg atggagcccc
     7741 ccgtgtagag gaggatgccg aacgccgcgc cgcccatcac gaggatgccg ttctgcgtca
     7801 cgagccggtc cgagaggagg ctgaaccggt gcgggaccca ggagtcgatc gccatgttcg
     7861 agaggacgcg cggcccgtcg aggaaccccg cctgccctcc gacgaggagg agggccccct
     7921 cggcgacgag cgtcgccagg aggaaggcgc cggcgaggga gccggggccg aacgcctccg
     7981 tcgcgagcct ctccgtcagg accgcgttga gcgtccgccc ggggacccgg tggacccccg
     8041 cgagcaggta gcagaggatg aggccgccgg cggtgagcgc gagcgacgtc cccatgtaga
     8101 ccatcgtccg gcggcccgtc ctcacgcgcg gctcgcgcag gatctgcagg ccgttcgaga
     8161 ccgcctcgat ccccgtgtag gtgccgccgc ccatcgagaa ggcgcggagg aggagcgcga
     8221 gagtgccgat ggcgccgagc gacgtccacg tctcccgggt ctcgcgggcg gtcgccgcca
     8281 cgacctcggg catcgcgccg acgtggctcc cgatcccgta ggcgatgagg agcgcgtgcg
     8341 tcacgaggaa gaggatgaag atcgggagga ggacgagaac cgactccttc actcccctga
     8401 ggttcaggac gacgagcgcg acgaggagga cgagggagac gacgagctgc tcccgctggg
     8461 ggacggcccg gggcgcgagc gagcagagcg ccgccacgca cgccgccacc tgcatggcga
     8521 tcgtcatcgc gtagtcgacg acgagcgccg agcccgagac gacgccggct ccccggccga
     8581 gcaggatcgt cgcgaccagg tagccgccgc cgcctgacgg gaagagggag atgatctggg
     8641 agtacgacgc ggagatgacg ccgatcgtga tcaccatggc gatcgcgagg aagaccgcca
     8701 ggtgcgaatg cgccccgagc gtgacgaatg cttcctccgg gccgtagcac gaggacgaga
     8761 gcccgtccgc gccgaggccg acccaggcca ggaacgccgc cagcgagaca cggtggaaga
     8821 tcttcggatc gagaggatcg cgctccttcc cgatgacgaa gcggcggacg gattcgagga
     8881 tcggcatgga cagccgacga ctgtagcggt ccgccaccgc ttcccggaag gctccaggct
     8941 ctccgccgag cgtccgcgcg cccgatcttt agccattcct tagacgcccg gtggacctcc
     9001 ggacgtccct ggacgactta gggtttcctt cgcgttcctt ccggacttcc tccgggaccc
     9061 ggcggcacct ttcccggcgt ggaagggaga tacgtcatgc cgctcctcgt cgccctcgcc
     9121 gccgggctcg tcctcgtcct cgccgacatc taccgcggga agcccggaga ccggctctga
     9181 gcggccgcct ccggccatgc cgaggcccgc gccgatgaac gacccgaccc tcgtcgtcct
     9241 caccctcctc ctcggggccc tcgcgctcct ctacgtccgc ggctgcgccg ccctcctcgg
     9301 cgaggaccgc gatgagcgct gagtcgctcg ccggcctcgt cctgtcgggc ctcgcgctcg
     9361 cctacctcct cttcgccctg ctgaaaccgg agaagctctg acgtgacggc cctcgcgttc
     9421 ctcaagatcg cggtctactt cctcctcgtc ctcgccgtga cgaagccgct cggcctctac
     9481 atgcgccggg tcttctccgg cgagcggacg ttcctcgacc ccgtcctcct cccggtggag
     9541 agggccctct accg
//
LOCUS       DAOTEZ010000139         9522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5086_length_9522_cov_8.088941, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000139 DAOTEZ010000000
VERSION     DAOTEZ010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9522
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5086_length_9522_cov_8.088941"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            454..2835
                     /locus_tag="P5164_08880"
     CDS             454..2835
                     /locus_tag="P5164_08880"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRY44045.1"
                     /translation="MYAPLVFTLMALAGSATASVRPVARTIEVRNEFTGALPDEMTVR
                     FLPAPPSDRSEVVVPVESSRISVSIPASVRALALSGTGFEDVTVSLGTLGGDGIQLRP
                     LAVVRVRAESTGGRNGTEEIRLFVQADREGKAREVPVLREAEFATAQIEAGEWRGAVA
                     VGKTSLVPVEFRVAPGQKLDLDTRKGIALRRVRARIVTPAKQGVRDIAPVWRSQRDGR
                     AAPDRDRSEDDRDAESQVEVVGSALAALSGRSDPDGFLPLVLPARSAGTLTLETKGYR
                     RVVLRQGEGAPAEIPELLVLSPLPEIEVVVPKLHAGEAATLSVRLALASSDRWNGDHE
                     RPPSAHVRAGESVVLKALADADHSIEVRDAVGRLAGIAVVSTRDRDWAGATVTRVLER
                     AQRQIRGRVLWNDEPLADCAIVGLLRRSGEDGDGGDRLVPTLPPDGIESLADAARTDS
                     SGEFVLTVNVPGRYSVVAFPPGFTTGKSARGSVDTEALEQKPVEVRFSGRYLSVRVVD
                     DETGEPIQGAQVEVKSKAAEDSGKAGAYSSTVKATDREGGVTALGLLGGPLVVTVSAK
                     GYAGRRLTPKPSSDLPPVTTEVRLKVGMERSIRCLDENGVPLSGVEVLGLSEEARGTP
                     FSPVQPLGHTDLRGTCTFDDQGPAFPHLVLLAAGKVIRVIPYFAGAEGDPAPVSCVLH
                     SIGFGPSVRLMAEEKGKVWPVSRLPVVASFEGLLLPWNQAVSRKAEMDGAPDYLETGP
                     DGGVGLARTLGAGSWELVGFHRRPQSLENPSLLRLGTIAFPVSKDEIVTIRRK"
     gene            2839..3225
                     /locus_tag="P5164_08885"
     CDS             2839..3225
                     /locus_tag="P5164_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44046.1"
                     /translation="MKRLLLLTTLLGAFVFAPVAEAAVKEGCNECRLGKCRAVAFGSF
                     GMDVCIEGTEEDVTWPPGVTKTCDVETGQVCKRESTEVTVTGDDPCPFWCWIFGDCSR
                     GDEDPLSSISAEHRNPANADGDECPY"
     gene            complement(3383..3730)
                     /locus_tag="P5164_08890"
     CDS             complement(3383..3730)
                     /locus_tag="P5164_08890"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HRY44047.1"
                     /translation="MTIYEKGLVTILVVDAVSILISIPLVLRKIGPNVVYGYRTRATL
                     SDPALWYEANALFGRRLIAACLVSALAALFLARPGLLAPDAFLPVSVVLLGAPVAVAG
                     VMTSRFVAQYVPR"
     gene            complement(3856..6099)
                     /locus_tag="P5164_08895"
     CDS             complement(3856..6099)
                     /locus_tag="P5164_08895"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRY44048.1"
                     /translation="MTLKRPRAILVAAIAAAALLAPPLAAAPAKRAATIADLHAVQGV
                     GEPVIAPDGRSIAFTVTTTDLPAVKRWTNLWRVDADGKNARALTVLDKRDASPDFSPD
                     GGTLAFLSTRSGTPQVWFLPVAGGEPEKKTDLPGGVGAFRFSPDGKRLFLVADVWPAC
                     GADLDCNRKKDDAMEKGKMKAVVADSLFVRRWDSWEDGKRTHILLLDLADPKAPLVDL
                     TPGDFDSPAFSAGGGADFDVSPDGKELAFTSLRDPFPAASTNVDLYVVPVDGTPEQRK
                     APRNLTAKNPAADGAPRYSPDGKLLAFKMQRTPGYESDRFLLAVFDRAAGTSRVLTEE
                     LDSWVGDYRFSPDGKKVFFTADVRGRTPLHELDLASGKIDVVTSTGFLDTFQIAPDGT
                     WAVLARRQMHLPTELYRLTLAGKERGKVTPLTSVNEKLLSEVALAPMEEVFVDSPTGK
                     KVQVLLVKPHDFDPAKKYPVIVNVHGGPQMQWADAFRLDAQVYPGAGYVVAFPNPHGS
                     TGWGQDYTAAISGDWDGKVMKDVLAVADWLSVQKWADKDRMGAMGWSWGGYAMMWLAG
                     HDHPFQALAAMMGVYDLRSMYSMTEELWFPEWDLKGTPWQNPEGYAKQSPSSYVTKFK
                     TPTLVLTGQKDFRVPYTQSLAFFTDLQKMGVPSRLVVFENGSHWPGWYDMVLYYAAHL
                     DWFHRYLGGPASPYDPKALAFNDVFGKEKDEAKDEGKAPAKDDAKPAPRKAVPPKPEE
                     KKVPANG"
     gene            complement(6137..6667)
                     /locus_tag="P5164_08900"
     CDS             complement(6137..6667)
                     /locus_tag="P5164_08900"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HRY44049.1"
                     /translation="MKRALPLLALLLAAPLAGQENGIPRVPISPRGAEEIAVGTGTAR
                     VEYGRPFAKGRKIFGGLVPWGKVWRTGANAATTLRLDVDVTLGTAALSKGTYTLYTLP
                     GPKEWQLIVNRQTGQWGTEYDPKQDVVRIPMKVLSRHDHLEAMTIDFLPEDAFRGSLR
                     ISWEKKTLVVPYAFRK"
     gene            6798..8480
                     /gene="ettA"
                     /locus_tag="P5164_08905"
     CDS             6798..8480
                     /gene="ettA"
                     /locus_tag="P5164_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HRY44050.1"
                     /translation="MAQQYIYTMVNLRKVVPPQREILKGIYLSFFPGAKIGVLGLNGA
                     GKSSLLKIMAGVDREFNGEAFPAEGTRVGFLQQDPWLDPAKTVLENVEAAVEPVKKLL
                     ARFDELGEKMGESLPDAEMDKVMAEYGKVQDAIEAANGWDLDRTLETAMDALRCPPPD
                     ADVTKISGGERRRVALCRVLLEKPDLLLLDEPTNHLDAESVAWLEQHLAEYPGTVVAV
                     THDRYFLDNVAQWILELDRGAGIPWKGNYTSWLDQKATRLAQEEKAESNKQKTLEREL
                     EWVRMAPRARHAKGKARLQAYEQLLAESGAESRGETNEIYIPAGPRLGDVVVRAEGLK
                     KAYGDKLLFDDLTFDLPKGGIVGVIGPNGAGKTTLFKMIMGLEKPDGGVLTVGETVFP
                     SYVDQNRDRPDPTKSVWEEVSGGEEKILVGRREMSSRAWCSMFNFKGADQQKRIGDLS
                     GGEMNRLHLARTVKKAGNLLLLDEPTNDLDVDTLRALEEALLSFAGCAVVISHDRWFL
                     DRVATHMLAFEGDSKTFWYEGNYQEYEADRHKRLGAAADMPHRIKYRKLTTG"
     gene            8544..8843
                     /locus_tag="P5164_08910"
     CDS             8544..8843
                     /locus_tag="P5164_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44051.1"
                     /translation="MSLARVTVTLPEEIVAEMDRSAANRSGFVLEAVTRELARRRRAA
                     LRASLSAPHPESLTIAEAGVGEWGRSLPPEDAEGLLDPSRGRGVRWVEGKGWKER"
     gene            8846..9205
                     /locus_tag="P5164_08915"
     CDS             8846..9205
                     /locus_tag="P5164_08915"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRY44052.1"
                     /translation="MRLERGTVVLVDLDPTVGHEQKSVRPCVVVSDPAVTSDQRYPLV
                     GVVPLTAAEGRGALYPRIPAGTGGLRKESWALTDQLRSVDKRRVRAAYGSIDAAAMES
                     IDEGLRLFLGLIPVEPA"
     gene            9294..>9522
                     /locus_tag="P5164_08920"
     CDS             9294..>9522
                     /locus_tag="P5164_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44053.1"
                     /translation="MIAFVTLFLGLFYGPAEIELSAAPGVARIELYVDGDLVSELTGP
                     WVAKLDLGQEIVPRELVAVAKDAEGRRVGEVR"
BASE COUNT         1470 a         3183 c         3423 g         1446 t
ORIGIN      
        1 gatcgtgacg agccccttct cgtagatcgt catggcgacc gcggacctcc cggccggctc
       61 ccgagtatag gaagcggcgg ggctgctcgg ccgctcggcc agacttggga agacgctctc
      121 agcgcaacgc ggttcgtcga tcgccaagcg acgacgctca cgccacgacc ggcggcggga
      181 gatccaggga gacctgcgcc acttccgacc cgacgtgcgc gagccgtgtc aggggccgtc
      241 tgtggcgtcg cgatcggggc caccctcgtg aacggcgctc tgcccccggg ggccagcttc
      301 ggaacgcctt ccctgatgaa cctgcggcgg gaggtcgagg ggaagcgctg accgtatgcc
      361 gctgaggtcg gcttgcccgt ttggcacgaa atgtcaattg acgggtcgtc ggtgggacac
      421 gcagcatctg cgttcggaaa tgcaggagaa gcgatgtacg cccctctcgt cttcacattg
      481 atggctctcg caggttcggc caccgcgtct gtacgaccag tagcccggac catcgaggtg
      541 agaaacgagt tcaccggagc tctgccggat gagatgaccg tccggttcct tcctgctccc
      601 ccgtccgaca ggagcgaagt cgtcgtgccg gtcgagtcgt cgcgaatctc cgtgtcgatt
      661 ccggcgtcgg tacgagccct tgccctttca ggcacaggat tcgaggacgt gaccgtcagc
      721 ctcggcaccc tcgggggcga cgggatccag ctgaggcccc tggccgtcgt ccgggtgcgg
      781 gccgagagca cggggggtcg taacgggacg gaagagatcc ggctcttcgt ccaggcggat
      841 cgagaaggga aggctcgaga agtccccgtc ctgagagaag cggagttcgc aacggcgcag
      901 atcgaggccg gcgagtggcg tggagcagtc gcggtcggaa agaccagcct cgtcccggtg
      961 gagtttcgcg tcgcgccggg acagaagctc gacctcgaca cgaggaaggg aatcgcgctt
     1021 cgtcgcgttc gagcgcgaat cgtcacgccg gcgaagcagg gcgtacgtga catcgctccc
     1081 gtgtggcgca gccagcggga cggtcgggca gcgcctgacc gtgaccgatc cgaagacgac
     1141 cgggatgcgg aatcgcaggt ggaggtcgtc gggtccgctc tcgcggcgct ctcgggtcgt
     1201 tcagatccgg atggtttcct ccccctggtc cttccggctc gatccgcagg cacgctgacc
     1261 ctcgagacga agggctacag gcgggtcgtc ctccggcagg gcgagggggc gccggcggag
     1321 atcccggagc tgctggtcct gtcgcccctg cccgaaatcg aagtcgtcgt cccgaagctc
     1381 cacgcggggg aggcggcgac cctctcggtt cggctcgctc tggcctcctc cgacaggtgg
     1441 aatggcgatc acgagaggcc tccgtcggcc cacgtccggg cgggcgaaag cgtcgtcctg
     1501 aaggccctcg ccgatgcgga tcactcgatc gaggtgcgcg atgcggtagg tcgtctggcc
     1561 ggcatcgcgg tggtctcgac tcgggacagg gactgggccg gcgcgaccgt tacgcgggtg
     1621 ctcgagcggg cgcagcggca gattcgcggt cgagtgctct ggaacgacga accgctcgcg
     1681 gattgcgcca tcgtcggtct gctgcgacgg agcggcgagg acggcgacgg aggcgaccga
     1741 ctcgtcccga ccttgccgcc cgacggcatc gagagcctcg ccgacgcggc ccggacggac
     1801 tcgtccggcg agttcgtcct gacggtgaac gtccctggca ggtattcggt cgtcgcgttt
     1861 cccccgggct tcaccaccgg caagtccgcc cgtggctccg tcgacacgga agcgctggag
     1921 cagaagccgg tcgaggtccg attctccggc cggtatctca gtgtgcgggt cgtcgacgac
     1981 gagaccgggg agccgattca gggagcgcag gtcgaggtga agtcgaaggc tgcggaggat
     2041 agcgggaagg ccggggccta ttcgtccacc gtgaaggcga cggaccggga aggcggcgtg
     2101 actgctctgg gcctgctcgg cggtcctctc gtcgtgaccg tcagcgcgaa gggctacgcc
     2161 ggccgccgtc tcactccgaa gccctcatcc gaccttccgc cggtgacgac cgaggtccgg
     2221 ctgaaggtcg gaatggagcg atcgatccgc tgccttgacg aaaacggagt tcccctctcc
     2281 ggagtcgagg tcctcggcct gagcgaggag gccaggggaa cgccgttctc tcctgtccag
     2341 cctctcgggc acactgatct ccggggaacc tgcacctttg acgaccaggg gccagcgttt
     2401 ccgcacctcg tcctcctcgc ggcggggaag gtcatccggg tcattccgta cttcgcgggc
     2461 gcggaggggg atccggcacc cgtctcctgc gttcttcact cgatcggctt cgggccgtcg
     2521 gtccggctca tggcagagga gaagggaaag gtgtggcccg tgtcacgcct gcctgtggtc
     2581 gcgtcgttcg aaggccttct gcttccgtgg aatcaagcgg tgagtcgaaa ggcagagatg
     2641 gacggggcac cggactatct ggagacgggg cccgatggcg gtgtcggtct cgccaggacg
     2701 ttgggcgccg gctcctggga gctcgtcggt ttccatcggc gtccccagag tctcgagaac
     2761 ccgagcctcc tgaggctcgg gacgatcgca tttccggtct cgaaagacga gatcgtgacg
     2821 atcaggagga agtagcgtat gaagcggctt ctgctattga cgacccttct tggggccttc
     2881 gtcttcgcgc ctgtcgctga agcagccgtc aaggaagggt gcaacgaatg ccggctaggg
     2941 aaatgccgag ccgtagcgtt cggcagcttc ggcatggacg tctgcatcga gggcaccgaa
     3001 gaggacgtca cgtggccgcc cggagtgacg aagacatgcg acgtcgaaac gggacaggtc
     3061 tgcaagagag aatccacgga agtgacggtc accggagacg atccctgtcc cttctggtgc
     3121 tggatcttcg gggactgttc gcggggcgac gaagaccccc tgagctccat cagcgccgag
     3181 cacaggaacc ccgccaacgc ggacggtgac gagtgtccgt attagcgtgc gacgcgttcc
     3241 gcgccccttg acgggtggag ccctacgccg ctccgagagg tagtcgcctc gtcaaagacg
     3301 agaaacgctt gcctgcccgc tcgtgacggg cacgcgccgg agtgctcgag agatgccgag
     3361 gccgcgcgcg gcctcggcat cgtcacctcg gcacgtattg cgccacgaac cgcgacgtca
     3421 tcacccccgc cacggcgacc ggcgcgccga ggaggacgac cgagaccggc aggaacgcgt
     3481 cgggcgcgag gagcccgggg cgcgcgagga agagcgccgc cagcgccgag acgaggcacg
     3541 cggcgatcag gcgtcggccg aagagcgcgt tcgcctcgta ccagagcgcc gggtcggaga
     3601 gcgtcgcgcg cgtccggtag ccgtagacga cgttcgggcc gatcttccgg agcacgagcg
     3661 ggatcgagat gaggatggag acggcgtcga cgacgaggat cgtgacgagc cccttctcgt
     3721 agatcgtcat ggcgaccgcg gacctcccgg ccagtcgtcg agtataggga gcgtccggcg
     3781 ccgccggaac gcggcagaaa cgcgacggcc gcgcctcgcg gcgcggccgt cggttcgggt
     3841 ctacggacgg gagggctacc cgttcgccgg gaccttcttc tcctccggct tcggcgggac
     3901 ggccttgcgg ggcgcgggct tcgcgtcgtc cttcgccggc gccttgccct cgtccttcgc
     3961 ctcgtccttc tccttcccga agacgtcgtt gaaggcgagc gccttcgggt cgtagggcga
     4021 cgccggtccg ccgaggtagc ggtggaacca gtcgaggtgg gccgcgtagt agaggaccat
     4081 gtcgtaccag ccgggccagt ggcttccgtt ctcgaagacg acgaggcgcg acggcacgcc
     4141 catcttctgg aggtcggtga agaaggcgag cgactgcgtg taggggacgc ggaagtcctt
     4201 ctgtccggtc aggacgagcg tcggcgtctt gaacttcgtc acgtacgaag acgggctctg
     4261 cttcgcgtac ccctccgggt tctgccaggg ggtccccttc aggtcccact cggggaacca
     4321 gagctcctcc gtcatcgagt acatcgagcg gaggtcgtag acgcccatca tcgcggcgag
     4381 cgcctggaac gggtggtcgt gcccggcgag ccacatcatc gcgtagccgc cccacgacca
     4441 gcccatcgcc cccatccggt ccttgtcggc ccacttctgc accgagagcc agtcggcgac
     4501 cgcgaggacg tccttcatca ccttgccgtc ccagtcgccc gagatcgccg cggtgtagtc
     4561 ctgcccccag ccggtcgagc cgtgcgggtt cgggaaggcg acgacgtagc cggcgcccgg
     4621 gtagacctgc gcgtcgaggc ggaaggcgtc ggcccactgc atctgcgggc cgccgtggac
     4681 gttcacgatc accgggtact tcttcgccgg gtcgaagtcg tgcggcttca cgaggaggac
     4741 ctgcaccttc ttcccggtcg ggctgtcgac gaagacctcc tccatcgggg cgagggcgac
     4801 ctcggagagg agcttctcgt tcacgctcgt cagcggcgtc accttgcccc gctccttgcc
     4861 ggcgagcgtc agccggtaga gctcggtcgg caggtgcatc tgccgccgcg cgaggacggc
     4921 ccacgtcccg tcgggcgcga tctggaaggt gtcgaggaag ccggtcgagg tgacgacgtc
     4981 gatcttcccg gaggcgaggt cgagctcgtg gagcggcgtc cggccccgga cgtcggcggt
     5041 gaagaagacc ttcttcccgt cgggggagaa gcggtagtcg ccaacccagg aatcgagctc
     5101 ctccgtcagg acgcggctcg tcccggcggc gcggtcgaac acggcgagga ggaagcggtc
     5161 cgactcgtag ccgggcgtcc gctgcatctt gaacgcgagg agcttcccgt cgggcgagta
     5221 gcgcggcgcg ccgtcggcgg cggggttctt cgcggtcagg ttcctcggcg ccttccgctg
     5281 ctccggcgtc ccgtccacgg ggacgacgta gaggtcgacg ttcgtcgagg cggccgggaa
     5341 cgggtcgcgg agcgacgtga aggcgagctc cttgccgtcg ggggagacgt cgaagtcggc
     5401 gccgccgccg gcagagaagg cgggggagtc gaagtcgccg ggcgtcaggt cgacgagcgg
     5461 cgccttcggg tcggcgaggt ccaggaggag gatgtgggtc cgcttgccgt cctcccagga
     5521 gtcccagcgg cggacgaaga gggagtcggc gacgaccgcc ttcatcttcc ccttctccat
     5581 cgcgtcgtcc ttcttccggt tgcagtcgag gtcggcgccg caggcgggcc agacgtcggc
     5641 gacgaggaag agcctcttgc cgtcgggcga gaagcggaaa gcgccgacgc cgccggggag
     5701 gtcggtcttc ttctcgggct cgccgccggc caccgggagg aaccagacct gcggcgtgcc
     5761 ggagcgggtc gagaggaacg cgagcgtccc gccgtcgggg gagaagtcgg ggctcgcgtc
     5821 gcgcttgtcg aggacggtca gcgcccgggc gttcttcccg tcggcgtcga cgcgccagag
     5881 gttcgtccag cgcttgacgg ccggcaggtc ggtcgtcgtc acggtgaacg cgatgctcct
     5941 gccgtcgggg gcgatcaccg gctcgccgac cccctggacc gcgtggaggt ccgcgatcgt
     6001 ggcggcccgt ttcgcggggg cggccgcgag cggcggggcg aggagggcgg cggccgcgat
     6061 cgcggcgacg aggatcgcgc ggggtctctt caaggtcatc ggtctgtctc ctccgggcgc
     6121 gcggggaggg cgcccgtcac ttgcggaacg cgtacgggac gacgagcgtc ttcttctccc
     6181 aggagatccg gagggagccg cggaaggcgt cttcggggag gaagtcgatc gtcatcgcct
     6241 cgaggtggtc gtgccgggag aggaccttca tcgggatccg aacgacgtcc tgcttcgggt
     6301 cgtactccgt cccccactgc ccggtctggc ggttcacgat gagctgccat tccttcgggc
     6361 cggggagcgt gtagagcgtg taggtcccct tcgacagggc ggccgtgccg agcgtgacgt
     6421 cgacgtcgag gcggagcgtc gtcgcggcgt tcgcaccggt ccgccagacc ttcccccacg
     6481 ggacgaggcc gccgaagatc ttccgcccct tcgcgaaggg gcgaccgtac tcgacgcgcg
     6541 ccgtcccggt cccgacggcg atctcctcgg cgccgcgggg cgagatcggg acgcggggaa
     6601 tcccgttctc ctgtccggcg aggggcgcgg cgaggaggag ggcgaggagg ggaagggctc
     6661 gtttcatgcg gtctccaagt ctcgacggcg ggccggggcc ggccgcccgt gggagtttac
     6721 gggaggggat gggggccggg cgccccggcg aggcgcggcc agcgcccccg ccgcacccct
     6781 gctacattcc gcccgccatg gcgcagcagt acatctacac gatggtcaac ctccgcaaag
     6841 tcgtcccccc gcagcgcgag atcctgaagg ggatctacct gtcgttcttc ccgggcgcca
     6901 agatcggcgt cctcggcctg aacggcgcgg ggaagtcgtc gctcctgaag atcatggcgg
     6961 gcgtcgacag ggagttcaac ggagaggcct tcccggcgga agggacgcga gtgggcttcc
     7021 tccagcagga cccgtggctc gaccccgcga agaccgtcct cgagaacgtc gaggcggccg
     7081 tcgagccggt gaagaagctc ctcgcgcgct tcgacgagct gggcgagaag atgggcgaga
     7141 gcctccccga cgccgagatg gacaaggtga tggcggagta cgggaaggtg caggacgcca
     7201 tcgaggccgc gaacggctgg gacctcgacc ggacgctcga gacggcgatg gacgccctcc
     7261 gctgcccgcc ccccgacgcc gacgtcacga agatctccgg cggcgagcgc cgccgcgtcg
     7321 cgctctgccg cgtcctcctc gagaagcccg acctcctcct cctcgacgag ccgacgaacc
     7381 acctcgacgc cgagtcggtc gcctggctcg agcagcacct cgccgagtac cccggcaccg
     7441 tcgtcgcggt cacgcacgac cgctacttcc tcgacaacgt cgcccagtgg atcctcgagc
     7501 tcgaccgcgg cgccggcatc ccgtggaagg ggaactacac ctcctggctc gaccagaagg
     7561 cgacccgcct cgctcaggag gagaaggccg agagcaacaa gcagaagacg ctcgagcgcg
     7621 agctcgagtg ggtgcggatg gccccgcgcg cccggcacgc gaaggggaag gcgcgcctcc
     7681 aggcctacga gcagctcctc gccgagagcg gcgccgagag ccgcggcgag acgaacgaga
     7741 tctacatccc ggcggggccg cgcctcggcg acgtcgtcgt ccgcgcggaa ggcctgaaga
     7801 aggcctacgg cgacaagctc ctcttcgacg acctgacgtt cgacctgccg aagggcggca
     7861 tcgtcggcgt catcggcccg aacggcgccg gcaagacgac gctcttcaag atgatcatgg
     7921 gcctcgagaa gcccgacggc ggcgtcctca cggtgggcga gacggtcttc ccctcctacg
     7981 tcgaccagaa ccgcgaccgc cccgacccga cgaagtccgt gtgggaggag gtctccggcg
     8041 gcgaggagaa gatcctggtc ggccgccgcg agatgtcgag ccgcgcctgg tgctcgatgt
     8101 tcaacttcaa gggggccgac cagcagaagc ggatcggcga cctctcgggc ggcgagatga
     8161 accgcctcca cctcgcccgc accgtgaaga aggccggcaa cctcctcctc ctcgacgagc
     8221 cgacgaacga cctcgacgtc gacaccctcc gtgccctcga ggaggcgctc ctctccttcg
     8281 ccggctgcgc cgtcgtcatc tcccacgacc gctggttcct cgaccgcgtc gcgacgcaca
     8341 tgctggcctt cgagggggac tcgaagacgt tctggtacga ggggaactac caggagtacg
     8401 aggccgaccg gcacaagcgc ctcggcgccg ccgccgacat gccgcaccgg atcaagtacc
     8461 ggaagctgac gacggggtag gacgggcccg cggctcaccg gagcggtgag atcctgagta
     8521 tgatcctacc ggaggccgcc cccatgagcc tggcccgagt cactgtcacg ctccccgagg
     8581 agatcgtcgc cgagatggac cgctccgccg cgaaccggag cgggttcgtc ctcgaggccg
     8641 tcacgcgcga gctggcgcgt cgtcgacggg cggcgcttcg tgcctcgctc tccgctccgc
     8701 atcccgagag cctcacgatt gccgaggccg gcgtcggcga atggggccgc tcgcttccgc
     8761 ccgaagacgc ggaggggctt ctcgacccct cccgcgggcg cggcgtgcga tgggtcgagg
     8821 gaaagggctg gaaggagcgc tgaggatgcg gctcgagcgc ggcaccgtcg tcctcgtcga
     8881 cctcgacccc accgtcggcc acgaacagaa gagcgtccgt ccctgcgtcg tggtcagcga
     8941 cccggccgtc acgagcgacc agcgctaccc gctcgtcggc gtcgtcccgc tcaccgccgc
     9001 cgaggggcga ggcgcgctct accctcgcat tcccgcagga acaggcggcc tccgcaagga
     9061 gtcctgggcg ctgaccgacc agctccgctc cgtcgacaag cggcgcgtcc gcgccgccta
     9121 cggctcgatc gacgcggccg cgatggagtc gatcgacgaa gggctccgcc tcttcctggg
     9181 gctgattccg gtagagcccg cgtaatctca ttcgctgcgc tccgcctctg tccccgcccg
     9241 gagacggccc ccggggaacg cgaattgctt gaactctgcc gggaggagtc cgcgtgatcg
     9301 ctttcgtgac gctgttcctc ggcctcttct acgggccggc cgagatcgag ctctccgccg
     9361 cgccgggcgt ggcgcggatc gagctctacg tcgacggcga cctcgtctcg gagctgacgg
     9421 ggccgtgggt cgcgaagctc gacctcgggc aggagatcgt tccgcgggag ctcgtcgccg
     9481 tggcgaagga cgccgagggg cgccgggtcg gagaggtccg gc
//
LOCUS       DAOTEZ010000140         9514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5093_length_9514_cov_5.912147, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000140 DAOTEZ010000000
VERSION     DAOTEZ010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9514
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5093_length_9514_cov_5.912147"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1998
                     /locus_tag="P5164_08925"
     CDS             <1..1998
                     /locus_tag="P5164_08925"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRY44054.1"
                     /translation="FADLATAQEVLGREGHLDRIDLVLPAGLGEADRTALEASVRDSL
                     PPGVTVGRPERRTATVDRMVRSFRVNLTALGLIALLVGTYFVYGTLSISVLRRRSDIG
                     TVRALGASSRSVFLVFLAEGAALGVLGSSLGLVLGAGMAKAALAAVGGTATDFYLTQA
                     APALALEPRALAIAFLAGLLTSILSALAPALEAAHVDPASTFRHGSVEASRRRAVKPL
                     AIAGACLLLLAALATRPGPVGGLPLFGFAAVFLVVAGVSLLAPFAVTTVARHADRLAV
                     RLLGVEARIARANLTGSLSRTAVAVAALSMGISMMVAVAVMVGSFRATVVDWVSQTLK
                     ADLFVAPESGRSHRALGRLSEEAIRLVASVPGVASVDPFLSFEATRDGAPYTIGSGRF
                     DLVAAHGDLPMADGSDTREVLARALARGEAVVSEPYSVKFGVSAGDTVTLPALAGPAV
                     LRVAGVYRDYSNDRGTVTIDRALFRRLFGMEGAVSLAVRLDPGVAPEEARRRLLAAAS
                     GRQALRVQTNAALREAALALFDRTFAVTWALEAVAIAVAVLGVFSTLLALVLERRREI
                     ALLRVLGASAARVARAVRLEAAALSALGILLGVAAGGAMGLVLVHVVNRQSFGWTIRT
                     DVPWLFLAGALALVLLATLAAASRPARLAAATDPAAVLREE"
     gene            2003..3178
                     /locus_tag="P5164_08930"
     CDS             2003..3178
                     /locus_tag="P5164_08930"
                     /inference="COORDINATES: protein motif:HMM:NF018805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HRY44055.1"
                     /translation="MSSPSRAPISARPARRLVASLRPAFALVALSLLSSTAPLAAEGG
                     RFRPLDPKATPVFPRDHGAHDDARVEWWYVTGHLVDGEGRRSGFQLTFFRTGLVDEPK
                     GARASAFAPRDLHLAHFARTDVDGGAFRYAERTHRDGPGGASARTTHLDVANEDWRLT
                     ELGGKLHLRASDRVGGVEETLTLLLTPEKPPVLHGENGLSRKAPEAEAVSRYVSFTRL
                     RAEGWATRGRDARSVTGSAWMDHEWGSGAIGKGTRGWDWFSVQLDDGRDLMIYQLRGE
                     DGARTLHSSGTLVERDGRATPLSPPEFSVEVTARWTSPRSKGTYPARWLLRIPKANLV
                     LEVVPVLADQELVTDRSTRVTYWEGACDVRAPGSGRPLGRAYVELTGYAGKGALGLF"
     gene            complement(3318..4649)
                     /gene="rseP"
                     /locus_tag="P5164_08935"
     CDS             complement(3318..4649)
                     /gene="rseP"
                     /locus_tag="P5164_08935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HRY44056.1"
                     /translation="MQISTNILAFVFVLSFLIFFHEFGHFLVARLFKFPVEVFSIGFG
                     KRLFGFKRNGTDYRVSLVPLGGYVKIIGLGPDESDVVDAGADQGLRGTRGQRFLVLLA
                     GPAVNLVLALLLTAGAFTIGVTVPKYADERPVVKHVDAGSPAEKAGVQVDDVVVSLSG
                     KPVTTWREVETHLGLAPRAEIPVVLERGGASVTVVIRPEPQTKYDIGYTGLNPWLAPI
                     IGNLVKGYPGEKAGLQVGDRIVSIGGVAIDGYFEVVRRVREASAAFEEGKPGPIAFEV
                     DRNGTRVALDVTPRLEGGSWRIGFTPRYELIKRKMPFLAAIGASWKENLRQTAAVGQT
                     IKRMVSGSGSIRQLSGPVDIAKFSGEAVRTGWAALLGFMGLLSLQLGLLNLLPIPLLD
                     GGQLMVLTFEGIFRRDFSLKFKERLLQVGFVFLVLLMVSVLAFDIAKNLGF"
     assembly_gap    4785..4884
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4885..5705)
                     /gene="dxr"
                     /locus_tag="P5164_08940"
     CDS             complement(<4885..5705)
                     /gene="dxr"
                     /locus_tag="P5164_08940"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRY44057.1"
                     /translation="MSAPRSVALLGATGSIGRSALSVFEALPERFRVVSMSAGSNLDV
                     LLPAIARFRPAVVSVKEREAADRVRREFPGLRVGFGEQGMIDVATHPEAEVVLGAVVG
                     AAGLPPAYEAVKLGKTLALANKEVLVVAGEAVTTAAAASGAAILPVDSEHCALHQALR
                     CGRPEEVDRLVLTASGGPFRKRPLETFDAITIEDALAHPTWKMGPKITIDSATMMNKG
                     LEVIEARWLFDVPPERIGVVIHPQSIVHSFVEWIDGSVIAQISPNDMRFPILYALT"
     gene            complement(5702..6523)
                     /locus_tag="P5164_08945"
     CDS             complement(5702..6523)
                     /locus_tag="P5164_08945"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRY44058.1"
                     /translation="MRRTREATAVVLIPLVLFVLVKGPLLSWAALAGLACAAALHEFH
                     GLIRCGGWLAPRALSFVLFAAIVGAAYLGSAPLLVAAAGAVLVAVPTAVLLAAPREPI
                     WLVSSSATAFATLYVGSGAAAIVALRGIGWEPVVFLLGIVWAGDSAAYYVGTRFGKRR
                     LAPVVSPKKSWEGFWGQVVAGAVFGALLALLLPRVPGTPAVAAAAGALLGAFLSVVAV
                     VGDLVESTFKRSCSVKDSGGLLPGHGGLLDRLDSLLYASPVLFGILALLPELRPR"
     gene            complement(6520..7344)
                     /locus_tag="P5164_08950"
     CDS             complement(6520..7344)
                     /locus_tag="P5164_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HRY44059.1"
                     /translation="MIEKDLVAAPGTPERALLETLDLGRLPRHVAVIMDGNGRWAKQR
                     HLPRVEGHRAGASAVRETVETAARLRLDALTLYAFSTENWKRPKAEVSTLFALLKEFL
                     KREFRSLVENDIRFRVVGRADGLDPSVRSALATALEATASCRGMVFSVALNYSGRAEI
                     ADAARALARDAAAGSLDPETIDEDAVARRLYTADLPDPDLLIRTSGELRISNFLLWQI
                     AYAEIHVTPVLWPDFRRRHLLEALLDYQRRERRYGGVAEEDASAPGVPPAPAALGA"
     gene            complement(7395..8186)
                     /gene="truA"
                     /locus_tag="P5164_08955"
     CDS             complement(7395..8186)
                     /gene="truA"
                     /locus_tag="P5164_08955"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRY44060.1"
                     /translation="MKARDEAADRADTGGARRRLELSYLGTHFEGWQTQDVDRTGSGP
                     PRTVQLVVEQALTRMHRAPVRVHAASRTDAGVHAEGQVVHFDVPPGGPLVPPEGLRRG
                     LNALLPWDVRVDGAAGVAAGWHARFDALGKRYVYRLRRGDHLPPFEGLREALASPRLD
                     LRAMRDAAARLVGRNDFGPFGLAGAPRRTTVRTLARLDVEERGPLVIVTAVGDGFLRG
                     MVRRLVGTLVEVGLGRTDAGEAFRRPGPTAPARGLTLEKVFYRAG"
     gene            complement(8310..8555)
                     /locus_tag="P5164_08960"
     CDS             complement(8310..8555)
                     /locus_tag="P5164_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44061.1"
                     /translation="MILVRNRNGTLYFLARDGDDALVSLKRSYPGRPLAILKKGEGLA
                     ILRVGDPLPGGEGDRGERLDIVYVVLPPAPEAFSFRA"
     gene            8706..>9514
                     /locus_tag="P5164_08965"
     CDS             8706..>9514
                     /locus_tag="P5164_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HRY44062.1"
                     /translation="MNPDRATLKVQELLQGIAREARSAGNPEVTPEHLAAALAAEASV
                     GIPLLTRIGVPASLVAGEASALAARLPKVQGVGAETRFSPSLVRVLDRAEEVAREFKD
                     DYVALEHLLLGLLRDGTSKAAEILKRHGVTEAAVLSALKEVRGSARVDDPNAEEKYQA
                     LKRYARDLTELARRGKLDPVIGRDDEIRRVMQVLTRRTKNNPVLIGDPGVGKTAVVEG
                     LARRVASGDVPESLKDKQVVALDLGAMVAGAKFRGEFEERLKAVIKEVEES"
BASE COUNT         1342 a         3487 c         3317 g         1268 t
ORIGIN      
        1 ttcgccgacc tcgccacggc ccaggaagtg ctcgggcgcg aaggccatct cgaccggatc
       61 gacctcgtcc tccccgccgg cctcggcgaa gccgaccgga cggccctcga ggcctcggtc
      121 cgcgactcgc tcccgccggg cgtcaccgtc gggcgccccg agcggcggac ggcgacggtc
      181 gaccggatgg tgcgctcctt ccgcgtgaac ctcacggcgc tcggcctcat cgcgcttctc
      241 gtcggaacct acttcgtcta cggcacgctc tccatctcgg tcctgaggcg gcggtccgac
      301 atcgggacgg tccgcgcgct cggagcctcg tcccgatcgg tcttcctcgt cttcctcgcg
      361 gaaggcgccg ccctgggcgt cctcggaagc tctctcggcc tcgtcctcgg ggccggcatg
      421 gcgaaggccg cgctcgccgc cgtcggcggg acggcgacgg acttctacct gacccaggcc
      481 gccccggcgc tcgccctcga gccgcgggcc cttgccatcg ccttcctcgc cggcctcctc
      541 acctcgatcc tctccgccct cgcgcccgcc ctcgaagcag cccacgtcga tccggcctcg
      601 acgttccggc acggctccgt cgaggcgtcc cggcggcggg ccgtgaagcc gctcgcgatc
      661 gccggcgcgt gcctcctcct cctcgccgcc ctcgccacgc gtcccgggcc ggtcggaggc
      721 cttccgctct tcggcttcgc ggcggtcttc ctcgtcgtcg ccggcgtctc gctcctcgcc
      781 cccttcgccg tcacgacggt cgcgcgacac gccgaccggc tcgccgtccg cctcctcggc
      841 gtcgaggcga ggatcgcccg cgcgaacctc accgggagcc tgtctcggac ggccgtcgcc
      901 gtggcggccc tctcgatggg gatctcgatg atggtcgccg tcgcggtgat ggtcggctcc
      961 ttccgcgcca ccgtcgtcga ctgggtctcg cagacgctga aggccgacct cttcgtcgcc
     1021 cccgagagcg gccggtcgca ccgcgcgctc gggcgcctct ccgaggaggc gatccgcctc
     1081 gtcgcgtccg tgcccggcgt tgcctccgtc gacccgttcc tctcgttcga ggcgacccgg
     1141 gacggagctc cctacacgat cggctcgggc cgcttcgacc tcgtcgccgc ccacggagac
     1201 ctgccgatgg ccgacgggag cgacacgcgc gaggtcctcg cacgcgccct cgcgcggggc
     1261 gaggccgtgg tctcggagcc ctactcggtc aagttcggcg tgagcgcggg agacacggtc
     1321 accctccccg cgctcgccgg cccggcagtt cttcgcgtgg cgggcgtcta ccgcgactac
     1381 tcgaacgacc gcggcacggt gacgatcgac cgcgcgctct ttcggaggct cttcgggatg
     1441 gagggtgccg tgagcctcgc cgtccgcctc gatcccggcg tcgcgccgga ggaggcgcgg
     1501 cggcggctcc tcgccgcggc gagcgggagg caggcgctcc gggttcagac gaacgcggcg
     1561 ctccgcgagg cggcgctcgc cctcttcgac cggacgttcg ccgtgacctg ggcgctcgag
     1621 gcggtcgcga tcgccgtcgc cgtcctcggc gtcttctcca cgctcctcgc gctcgtcctc
     1681 gagcggcggc gcgagatcgc gctcctgcgc gtcctcgggg cctcggccgc gcgcgtcgcc
     1741 cgcgccgtcc ggctcgaggc ggccgcgctc tccgcgctcg gcatcctcct cggcgtcgcc
     1801 gcgggcggcg cgatgggcct cgtcctcgtc cacgtcgtca accgccagtc gttcggctgg
     1861 acgatccgga ccgacgtgcc gtggctcttc ctcgcgggcg cgctcgcgct cgtcctcctc
     1921 gcgacactcg ccgccgcctc gcgccccgcc cgcctcgccg cggcgaccga ccccgctgcg
     1981 gtcctcaggg aggagtagcc cgatgtcgtc tccttctcgc gccccgatct ccgcccggcc
     2041 cgcccgcagg ctcgtggcgt ctcttcggcc ggccttcgcc ctcgtcgcgc tctcgctcct
     2101 ctcctccacc gccccgctcg ccgccgaagg cggccgcttc cgccccctcg acccgaaggc
     2161 cacgcccgtc tttccgcgcg accacggcgc gcacgacgac gcccgcgtcg agtggtggta
     2221 cgtcaccggc cacctcgtcg acggcgaggg gcgccggtcg gggttccagc tcacgttctt
     2281 ccggaccggt ctcgtcgacg agccgaaggg ggcccgagct tcggccttcg ccccgcgcga
     2341 cctccacctc gcccacttcg cacggaccga cgtcgacgga ggggcgttcc gctacgccga
     2401 gcggacccat cgcgacgggc cgggaggcgc ctcggcccga acgacgcacc tcgacgtcgc
     2461 gaacgaggac tggcggctca ccgagctcgg cgggaagctc cacctccggg cgagcgaccg
     2521 ggtcggcggc gtcgaggaga cgctcacgct cctcctcacg cccgagaagc cgcccgtcct
     2581 tcacggcgag aacggcctct cgaggaaggc ccccgaggcg gaggccgtca gccgctacgt
     2641 cagcttcacg cgcctccgcg cggaggggtg ggcgacgcgc ggaagagacg cccgctccgt
     2701 gaccgggagc gcctggatgg accacgagtg gggctcgggc gcgatcggga aggggacccg
     2761 cgggtgggac tggttctccg tgcagctcga cgacgggcgc gacctgatga tctaccagct
     2821 ccgcggcgag gacggcgcgc ggacgctcca ttcgtcgggg accctcgtcg agagggacgg
     2881 ccgcgcgacg cccctctccc ctccggagtt ctccgtcgag gtgacggcgc gctggacaag
     2941 cccccgctcg aaggggacct acccggcgcg gtggctcctg cgcatcccga aggcgaacct
     3001 cgttctcgag gtcgttcccg ttctcgcgga ccaggagctc gtcacagacc gctccacccg
     3061 cgtcacctac tgggaagggg cctgcgacgt ccgggctccc ggctccggcc gccccctcgg
     3121 tcgcgcctac gtcgagctga ccggctacgc cggcaagggc gccctcggcc tcttctgacg
     3181 gacctcgaag ccccgcaccg gtcgcaccgg ggggccgtgt ctgcactcat gtattgttta
     3241 tcaaacaata catgagtgta gacacggccc cgaacttgcg gcccggaacg gcttcgcccg
     3301 cggcgttcgt ccgggggtca gaagccgagg ttcttggcga tgtcgaaggc gaggaccgag
     3361 accatcagga ggacgaggaa gacgaagccc acctggagga gccgctcctt gaacttcagg
     3421 gagaaatcgc ggcggaagat cccttcgaac gtcaggacca tcagctggcc accgtcgagg
     3481 agcgggatcg gcaggaggtt caggagaccg agctggagcg agagcaggcc catgaagccg
     3541 aggagcgccg cccatccggt gcggaccgcc tcgcccgaga acttcgcgat gtcgaccggg
     3601 ccggagagct ggcggatcga gcccgagccc gacaccatcc gtttgatcgt ctgcccgacc
     3661 gccgcagtct gacgcaggtt ctccttccag gaggcgccga tcgccgccag gaacggcatc
     3721 ttccgcttga tcagctcgta cctgggcgtg aagccgatcc tccacgaccc gccctcgagc
     3781 cgcggggtga cgtcgagggc gacgcgcgtc ccgttccggt cgacctcgaa ggcgatcggc
     3841 ccgggcttcc cctcctcgaa cgcggcggac gcctcgcgaa cgcgccgcac gacctcgaag
     3901 tagccgtcga tggcgacgcc cccgatcgag acgatccggt cgccgacctg gagccccgcc
     3961 ttctctccgg ggtatccctt cacgaggttc ccgatgatcg gcgcgagcca cgggttcaga
     4021 ccggtgtagc cgatgtcgta cttcgtctgg ggctcggggc ggatcacgac ggtcacggac
     4081 gccccgcccc gctcgaggac gacggggatc tcggcccgcg gcgccaggcc gaggtgcgtc
     4141 tctacctcgc gccacgtcgt gaccggcttg ccggagagcg agacgacgac gtcgtcgacc
     4201 tgcacgcccg ccttctcggc cgggctcccc gcgtcgacgt gcttcacgac cggcctctcg
     4261 tcggcgtact tcggcaccgt cacgccgatc gtgaacgccc ccgccgtcag gaggagggcg
     4321 aggacgaggt tcaccgcggg tcccgcgagg aggacgagga agcgctgccc gcgcgtgccg
     4381 cgcagcccct gatccgcgcc ggcgtccacg acgtcgctct cgtcggggcc gaggccgatg
     4441 atcttcacgt acccgccgag cggcacgagg gacaccctgt agtccgtccc gttcctcttg
     4501 aacccgaaga ggcgcttccc gaagccgatc gagaagacct cgaccgggaa cttgaagagc
     4561 cgcgccacga ggaagtgccc gaactcgtgg aagaagatga ggaacgagag gacgaagacg
     4621 aaggcgagga tgttcgtgga gatctgcatc ggaccctttg cttacgccga agccggcgcg
     4681 acggttctcg gggaagcaag cggcgcgccg gaggccacga ggtcggccgc ccggcgccgg
     4741 gcctcccggt cggcctcgag gacggcctcg agggtcgacg ccggnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnngtgagc gcgtagagga tcgggaagcg catgtcgttc
     4921 ggcgagatct gcgcgatgac cgagccgtcg atccactcga cgaaggagtg gacgatcgac
     4981 tgggggtgga tgacgacgcc gatccgctcc ggcggcacgt cgaagagcca ccgcgcctcg
     5041 atcacctcga gccccttgtt catcatcgtg gccgagtcga tcgtgatctt cggccccatc
     5101 ttccaggtcg ggtgggcgag ggcgtcctcg atcgtgatcg cgtcgaacgt ctcgagcggg
     5161 cgcttccgga acggcccgcc cgacgccgtc aggacgagcc ggtccacctc ctccggccgg
     5221 ccgcagcgaa gcgcctggtg gagggcgcag tgctccgagt cgaccgggag aatcgcggct
     5281 cccgaggccg ccgcggccgt cgtcaccgct tccccggcga cgacgaggac ctccttgttc
     5341 gccagggcga gcgtctttcc gagcttcacc gcctcgtacg ccggagggag cccggccgca
     5401 ccgacgacgg ccccgaggac gacctcggcc tcgggatgcg tcgcgacgtc gatcattccc
     5461 tgctcgccga agccgacacg caacccgggg aactcgcgcc ggacccgatc ggccgcctcc
     5521 cgctctttca cggagacgac cgccggccgg aaccgggcga tcgccgggag aagcacgtcg
     5581 aggttcgagc cggccgacat cgagacgacg cggaagcgct ccggcagcgc ctcgaagacc
     5641 gaaagcgccg accggccgat cgagccggtg gcgccgagga gggccacgct tcggggggcg
     5701 ctcatcgcgg ccgcagctcc ggcagaaggg ccaggatccc gaagaggacg ggcgaggcgt
     5761 agaggaggga gtcgagtcga tcgaggaggc cgccgtgccc cggaaggagc cccccggaat
     5821 ccttcaccga gcagcttcgc ttgaaggtcg actcgacgag gtcgccgacg acggcgacca
     5881 ccgacaggaa ggccccgagg agggcccctg ccgcggcggc gaccgccggc gtcccgggga
     5941 cgcgcggcag gagaagcgcg aggagcgcac cgaacacggc cccggcgacc acctgtcccc
     6001 agaatccctc ccagctcttc ttcgggctga cgaccggggc caggcgcctc ttcccgaacc
     6061 gggtgccgac gtagtaagcg gccgagtccc cggcccagac gatcccgagc aggaagacga
     6121 ccggctccca cccgatcccc cgaagcgcga cgatcgcggc ggcgcccgat ccgacgtaga
     6181 gggtcgcgaa ggcggtcgcc gaggacgaga cgagccagat cggctcgcga ggcgccgcaa
     6241 ggagcacggc cgtcgggacg gcgacgagga ccgctccggc cgccgcgacg agcagcggtg
     6301 ccgagccgag gtacgcggcg ccgacgatcg cggcgaagag aacgaagctg agcgcccgcg
     6361 gggcgagcca gccgccgcag cggatgaggc cgtggaactc gtggagggcg gccgcgcagg
     6421 cgagcccggc gagggcggcc cacgaaagga gcggcccctt cacgaggacg aagaggacga
     6481 gcgggatgag gaccaccgcc gtcgcctcgc gggtgcgcct cacgcgccga gcgccgccgg
     6541 cgcgggcggg acgcctgggg cggacgcgtc ctcctccgcg acgcctccat agcgccgctc
     6601 ccgtcgctgg tagtcgagga gggcctcgag gagatgtcgc ctgcggaagt cgggccagag
     6661 gacgggcgtc acgtggatct ccgcataggc gatctgccag aggaggaagt tcgagatccg
     6721 gagctcgccg ctggtccgga tgaggaggtc ggggtcgggg agatccgccg tgtagagacg
     6781 gcgggcaacg gcgtcctcgt cgatcgtttc cgggtcgagg cttccggccg cggcgtcgcg
     6841 ggcgagcgcc cgggcggcgt cggcgatctc ggcccgcccg gagtagttca gggcgacgct
     6901 gaataccatc ccccggcacg aggcggtcgc ctcgagcgcg gtcgcgagcg ccgaccggac
     6961 cgaggggtcg aggccgtccg cgcgcccgac gacccggaag cggatgtcgt tctcgacgag
     7021 ggaccggaac tccctcttga ggaactcctt caggagagcg aagagcgtcg agacctcggc
     7081 cttcgggcgc ttccagttct cggtcgagaa ggcgtagagg gtgagggcgt cgaggcgaag
     7141 gcgagcggcc gtctcgaccg tctcccggac ggcggacgcg ccggcacgat gcccctcgac
     7201 acggggcagg tgccgctgct tcgcccacct cccgttgccg tccatgatga cggcgacgtg
     7261 ccgcgggagg cgtccgaggt ccagcgtttc cagcagggcg cgctcggggg tgcccggcgc
     7321 ggcgacgagg tccttctcga tcatctgtcc gacagccgga ccggaatcgt ccgggaccgg
     7381 ctgaatcttc aatcttaccc tgcccggtag aagaccttct cgagcgtcag gccgcgggcc
     7441 ggggccgttg gaccgggacg ccggaacgcc tccccggcgt cggtccgccc gaggccgacc
     7501 tccacgaggg tcccgacgag ccgccggacc atgccccgga ggaaaccatc gccgacggcg
     7561 gtgacgatga cgagcgggcc gcgctcctcc acgtcgagcc gggcgagggt ccgcacggtc
     7621 gtccggcgcg gcgctccagc cagaccgaag ggcccgaagt cgttccgccc gacgagccgg
     7681 gcggcggcgt cccgcatggc gcgcaggtcg aggcgcggcg aggcgagcgc ctcccgcagc
     7741 ccctcgaacg ggggaaggtg gtccccccgg cgaaggcggt agacgtagcg cttgccgagg
     7801 gcgtcgaagc gggcatgcca gcccgctgcg actccggctg ccccgtccac ccggacgtcc
     7861 cagggcagga gcgcgttcag accgcggcga aggccctcgg gcgggacgag cggcccgccg
     7921 ggcgggacgt cgaagtggac gacctgcccc tccgcatgga cgcccgcgtc cgtgcggctc
     7981 gcggcgtgga cccgcaccgg ggcgcgatgc atcctcgtga gcgcctgctc gacgacgagc
     8041 tgaaccgttc ggggggggcc ggaccccgtc cggtcgacat cctgggtctg ccacccttcg
     8101 aaatgggtcc cgagatagga aagctcgaga cggcgcctcg cgccgccggt gtcggccctg
     8161 tcggcggctt cgtctcgagc cttcacgctc cgatcatagg gtcgcccggc tcgtcgggcg
     8221 gtccggaccc ggccgtccca gcgcctcgcg gctctcgcga atcgccggcc gacggcgcct
     8281 gcgcgcggcc gcggtcggag accgtccggt caggcgcgaa aggagaaggc ttccggggcg
     8341 ggcgggagga cgacgtacac gatgtcgagc cgttcccctc tgtccccctc tccgcccggg
     8401 agggggtcgc cgaccctcag gatggccagc ccttcgccct tcttcaggat cgccagaggc
     8461 cgccccgggt aggagcgctt gagggagacg agggcatcgt caccgtcccg cgcgaggaag
     8521 tagagcgtgc cgttccggtt tcggacgagg atcatgcagg tgggaaccta gctccggtgg
     8581 ctgctgcgcc gcatcacccg aacgggtgaa actgttacat gccacaaaat ctacagacgc
     8641 actgtagaaa tctaacttga caataacgcg cgcagatact agattcccga gccggaggat
     8701 tccccatgaa ccccgaccgc gccacgctga aagtccagga gcttctccag gggatcgccc
     8761 gggaggcccg ctcggccggg aacccggagg tcacccccga gcacctcgcc gcggcgctag
     8821 ccgcggaggc atcggtcggg atccctctcc tcacccggat cggcgttccg gccagcctcg
     8881 tcgcgggcga ggcgtccgca ctcgccgctc ggctcccgaa ggtccagggg gtcggggccg
     8941 agacgcgctt ctccccatcc ctcgttcggg tcctcgaccg cgccgaggaa gtcgcccgcg
     9001 agttcaagga cgactacgtc gccctcgagc atctcctcct cggcctcctg cgagacggga
     9061 cgtcgaaggc ggccgagatc ctgaagaggc acggcgtcac cgaagccgcc gtgctgtccg
     9121 ccctcaagga ggtccgcggc agcgcccgcg tcgacgaccc gaacgccgag gagaagtacc
     9181 aggcgctgaa gcgctacgcg cgcgacctga ccgagctcgc ccgccgcggc aagctcgacc
     9241 cggtcatcgg ccgcgacgac gagatccgtc gcgtgatgca ggtcctgacg cgccggacga
     9301 agaacaaccc ggtcctcatc ggcgacccgg gcgtcggcaa gaccgccgtc gtcgaggggc
     9361 tcgcgcgccg cgtcgcctcg ggcgacgtgc cggagagcct gaaggacaag caggtcgtcg
     9421 ccctcgacct cggcgcgatg gtcgccgggg cgaagttccg cggcgagttc gaggagcggc
     9481 tcaaggcggt catcaaggag gtcgaggagt cgga
//
LOCUS       DAOTEZ010000141         9507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5095_length_9507_cov_5.902666, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000141 DAOTEZ010000000
VERSION     DAOTEZ010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9507
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5095_length_9507_cov_5.902666"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="P5164_08970"
     CDS             complement(<1..833)
                     /locus_tag="P5164_08970"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HRY44063.1"
                     /translation="MSGSPLPAEVVVVGNAGVDTCVFPYGDEIDWGVEANFTETLDTV
                     GQAGGYASRGYARLGRRAAFVGHVGADHDGDLVRSTLARDGVDTRVLLTDPKGTARSV
                     NFMYRDGRRKNFYDGKGHMGLVPDLDLCRSVMEGARLAHVNLPNWARRLLPLARELGL
                     TVACDLQDVVDPADPYRRDFVDSADVVFCSAANHEPEHLAGELWARRPGLVIVVGMGA
                     RGCALGVAGRVRRFPPVPDERPVVDTNGAGDGLAVGFLTSYVLEGRPLEESVFRGQLA
                     AR"
     gene            complement(830..1369)
                     /locus_tag="P5164_08975"
     CDS             complement(830..1369)
                     /locus_tag="P5164_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HRY44064.1"
                     /translation="MAILASLAALAGCSTSTTERLGLPPLETVDHVDLAKYVGTWYEI
                     AAIPQSFQKGCVATTATYTLRADGDIDVLNRCRKDTLDGPEKSARGRARVVDGATNAK
                     LEVSFFRPFWGDYWIVDVGADYEYAVVGHPGRDYLWILGRATTMPEATYEGILARLKA
                     QGYDTSRLKRTLHGSGRRT"
     assembly_gap    1370..1469
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1669..4413)
                     /locus_tag="P5164_08980"
     CDS             complement(1669..4413)
                     /locus_tag="P5164_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRY44065.1"
                     /translation="MKVDAGARLGPYEVLAHLGEGGMGDVWRARDTRLGRELALKLLP
                     PAFAAEPERLARFEREARLLASLNHPGIAHLYGFEATPLPDGTTAHVLVMELAEGEDL
                     AERMKRGRIPLEEALPIARQVAEALEAAHEKGIVHRDLKPQNVKLGPDGTVKVLDFGL
                     AKALDPRAASGGEAVGPSAATESPTLMNSPTLTSAGTAVGVILGTAAYMAPEQAKGLA
                     VDRRADVWAFGVLVWEMLAGRRLFEGDSVAESLASVLKGPIELSALPASTPAQLKQLL
                     RRCLERNPRNRLHDVADARLVLVDLEAGVADEAPAVAAAPPPRDRRRELVARGFAAVA
                     LVAAGGLFLGRGSDEPVVPRVIRATLPLPAGVSIELDGERAGMPAVSPDGRRVAFGAR
                     EGSGPMRIWVHDLATAVARPLPGTEEGYRPFWSPDGRRIGFFTWGHLATTPADGGAVA
                     RLAPARDSRGGTWSPDGTILFAPFATGPLLAISERGGEVREVTGSREALGTATHRFPQ
                     FLPDGEHFVFLDRSGRVGSVAGGVLLAKLGETGPTRRLLEGATNAVYADGHLLFVRDG
                     ALVAQPFDAKSRALSGEPKVLVGDLLFNRRFAYGVFSASEAGVLVFLTGKQRDLSRLV
                     WRDRSGRRLGELGTPGNLSGFGGLALSRDGRWAAVGRVDEAQSDADIWLYDVARGTET
                     RLARPGSDESDPLFSSDGGTLYFGSAGDEGSSVVRRDLASGAETTLFSSKEIYSTGPM
                     SLSPDGAAMTCEERSRLAYADLVLRPSHGEGETKVLVRSPHDDAYGQVSPDGRWLLWA
                     SDDSGRYEVYVAPFPGGGSRVQVSREGGTQPRWNPAGGEILFKTPGNVLTAVRIETAS
                     GTISVGAPAPLFPIVEFTGWTYDVSADGQRFLVREPLAEGDASPMTLLTDWTALAGAR
                     "
     gene            4594..5883
                     /locus_tag="P5164_08985"
     CDS             4594..5883
                     /locus_tag="P5164_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY44066.1"
                     /translation="MGSRILFVLPGWPKGRLWGEMSFRFPSLSLAVVAAATPEKWETA
                     LHDEGFGPVDLDDPAGVVALTGMTAQATRAYEIADAFRARGKTVVMGGFHASNLPAEA
                     LQHVDAVVVGEAESVWPRLLSDLEEGRLQKVYRPDGPVDTAAIPVARRSIFDGKGYLL
                     TNTIQTTRGCPYDCEFCSVTAYFGRKYRKRPVETVLAELAEMKKRGSYAFFVDDNIVV
                     DRGYSLKLFRGMKGLGMKWLSHASLDLAEDPELLTAMGEAGCIGLFVGFESLDEKALA
                     AMGKKTNHVATYLDAVKAFRDSGIGILGSFVLGLDGDGPDVFERTLRFCESARLPAAI
                     FPILTPYPGTKVRERLLAEDRILSSDWRNYDMEHVNFRPKGMTVDELQAGYDLLMKEF
                     YSFGSMWKRLGWHRSLPAFGPMNLGFRSALKRKVADA"
     gene            5887..7320
                     /locus_tag="P5164_08990"
     CDS             5887..7320
                     /locus_tag="P5164_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid biosynthesis B12-binding/radical SAM
                     protein"
                     /protein_id="HRY44067.1"
                     /translation="MRRKGVPGVKVLMISANRERDPSPVFPLGLAYLAGPVERAGHET
                     RLLDLCFAADVPEAVRGAVEEMRPDVVLVSIRNLDNVTWPVCRSYVEGVREVVALCRD
                     AAPVVLGGSAFSLEPESFLEATGARCGVVGEGEEVVGPLLERIAAGLPLAGLPGVVEP
                     GGDGWTAPRTPATVGAPSRGSFDVSRYLTEGGAANVQTKRGCPFTCVYCTYPVLEGSR
                     VRTRPAAEVVRELRTLVDEHGVDYVEFCDDIFNFPEAAARELCEAMIADGLRLSWSAF
                     VNPGHLDARLVDLMARAGCDAVEIGTDSGSPAMLRSLGKSFTVEDVRAASRLCRDAGL
                     ATAHYLLFGGPGEDDATVAESVALMDELAPEAVIVMVGIRVYAGTDLHATAVREGVVR
                     ADQCLLEPVFYPDESRLRKIAERVAELAAARRTWIVPGLDVNTSSGVLEMLRRRHPRG
                     PLWKVLGQRESWRGRRRAAGETGPRAV"
     gene            complement(7381..8211)
                     /locus_tag="P5164_08995"
     CDS             complement(7381..8211)
                     /locus_tag="P5164_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRY44068.1"
                     /translation="MPHAPVDLARPEFPRSARYDAEFLLDNQMGPCAIWLLEWLCERF
                     PLAPGARVLDLGCGKAMTSVFLAKEYGAKVHAVDLWMSPDKNWERVAAAGAADLVCPV
                     RAEAHTLPFAAAYFDAIVSIDAYQYFGTDLLYLDYLTRFLRPGGRLGVVVPAVVRPLS
                     DGVPPWLTEPQSNGQAFWEEGCRAFLTAEAWTEIWRGSPCLTDVSAELQPDGWRHWAD
                     FERAIEAAGKGIFPSCAEALEKDAGRTIGFVRLLATRTGTSAENLYDPALGIKAGAER
                     "
     gene            8302..8718
                     /locus_tag="P5164_09000"
     CDS             8302..8718
                     /locus_tag="P5164_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRY44069.1"
                     /translation="MAEPKTKKTDASVEAFLDAVADPGRREDARAVLALMKRITGEEP
                     KMWGPSIVGFGSYTYTYASGRTGDWPIAGFSPRKQALTLYVMPGFEKDADLMKRLGKH
                     KTGKSCLYLNRLADVDAGVLEELVRRSVERMRNRRA"
     gene            complement(8771..>9507)
                     /gene="selD"
                     /locus_tag="P5164_09005"
     CDS             complement(8771..>9507)
                     /gene="selD"
                     /locus_tag="P5164_09005"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HRY44070.1"
                     /translation="VAFPSRRLPLSVLERILAGAAEVAREAGIEILGGHTVDDTEPKF
                     GLAVTGVVRPDRVVTNAGARPGDALVLTKPIGTGVLATAVKRGLAGEEAGRRLVTTMR
                     TLNRAAAEAMVEAGASACTDVTGFGLLGHLREMAAASGVDAEIEAASVPLLPDVRELA
                     AADGVPGGSLENLEHVAPHVDFAPGLSRVDRLLLADAQTSGGLLVALPEKRCEALLSS
                     LRARGVADARRIGRIAGAGTGRIRVR"
BASE COUNT         1291 a         3348 c         3510 g         1258 t
ORIGIN      
        1 cgggccgcga gctggccccg aaagacggac tcctcgagcg ggcggccctc cagcacgtag
       61 ctggtgagga agccgaccgc gagcccgtct ccggccccgt tcgtgtcgac gacgggccgc
      121 tcgtcgggga cgggcggaaa tcgacgaacc cggcccgcga ctccgagggc gcagccgcga
      181 gcgcccattc cgaccacgat gaccagaccg gggcgccgcg cccagagctc gccggcgagg
      241 tgctccggct cgtggttcgc cgccgagcag aagacgacgt cggcggagtc gacgaagtcg
      301 cgccggtacg ggtccgccgg gtcgaccacg tcctgaaggt cgcacgccac cgtgaggccc
      361 agctcacggg cgagcggcag cagccgccgg gcccagttcg ggaggttgac gtgggccagg
      421 cgcgcccctt ccatcacgct ccggcagagg tccaggtcgg ggacgaggcc catgtggccc
      481 ttgccgtcgt agaagttctt gcggcgcccg tcgcggtaca tgaagttgac gcttcgggcg
      541 gtccccttcg ggtccgtgag gagaacgcgg gtatcgaccc cgtctcgggc gagggtggag
      601 cggacgaggt cgccgtcgtg gtcggcgccc acgtgaccga cgaaagccgc gcgccgtccg
      661 agccgggcgt agccgcggct cgcgtagccg ccggcctgtc ccaccgtgtc gagggtctcg
      721 gtgaagttcg cctcgacacc ccagtcgatc tcgtcgccgt acgggaaaac gcacgtgtcg
      781 acaccggcgt ttccgacgac gacgacctca gcggggagtg gcgacccgct catgtccgcc
      841 tcccggatcc gtggagcgtg cgcttcagcc gggacgtgtc gtagccctgg gccttcagcc
      901 gcgcgaggat tccctcgtag gtcgcctcgg gcatcgtcgt ggccctgccg agtatccaga
      961 ggtagtcgcg gcccgggtgg ccgacgacgg cgtactcgta gtcggctccg acgtcgacga
     1021 tccagtagtc gccccagaac gggcggaaga agctcacctc gagcttcgcg ttcgtggcgc
     1081 cgtccacgac ccgggcgcgg cctcgcgccg acttctccgg accgtcgagg gtgtccttgc
     1141 ggcagcggtt caggacgtcg atgtcgccgt cggcgcggag cgtgtaggtc gcggtcgtgg
     1201 ccacgcagcc cttctggaag ctctgcggaa tggcggcgat ctcgtaccag gtcccgacgt
     1261 acttcgcgag gtcgacgtgg tccaccgtct cgaggggcgg cagcccgagt cgttctgtcg
     1321 tgctcgtcga gcacccggcg agggcagcca gcgaggcgag gatcgccagn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc tgtcgtgctc gtcgagcacc cggcgagggc
     1501 agccagcgag gcgaggatcg ccaggggtct caattccatc ggctccggat cgagcatacg
     1561 cgcgcgacgc agggccggat tcgggagttg gccccgacgg tcgccttcgt catccgacgt
     1621 cgaggagccc gcccgacgcc gtcctccggc cgtcgacgac gagggccctc agcgcgcccc
     1681 cgcgagcgcc gtccagtccg tgaggagggt catcggcgag gcatcgccct cggccagcgg
     1741 ctcgcgcacg aggaagcgct ggccgtcggc cgagacgtcg tacgtccagc cggtgaactc
     1801 gacgatgggg aagaggggcg ccggggcgcc gaccgagatc gtcccggagg cggtctcgat
     1861 gcgcactgcg gtcaggacgt tgccgggcgt cttgaagagg atctcgccgc ccgccgggtt
     1921 ccaccgcggc tgcgtgcctc cctcgcgcga gacctgcacc cgcgatcccc cgccgggaaa
     1981 cggcgccacg tagacctcgt accggcccga gtcgtcggaa gcccagagga gccaccgccc
     2041 gtcgggcgag acctggccgt aggcgtcgtc gtggggcgaa cggacgagga ccttcgtctc
     2101 gccctcgccg tgcgacggcc ggagcacgag gtcggcgtag gccaggcggc tccgctcctc
     2161 gcaggtcatc gcggctccgt ccggggagag cgacatcggc cccgtgctgt agatctcttt
     2221 ggacgagaag agcgtcgtct ccgctccgct ggcgaggtcg cggcggacga cggacgaccc
     2281 ctcgtctccg gccgagccga agtagagggt gccgccgtcg gaggagaaga gcgggtcgct
     2341 ctcgtcggag cccgggcggg cgaggcgggt ctcggtcccg cgggcgacgt cgtagagcca
     2401 gatgtcggcg tcgctctgag cctcgtcgac ccgcccgacg gccgcccatc ggccgtcgcg
     2461 cgagagggcg aggccgccga acccggagag gttgcccggc gtgccgagct cgccgaggcg
     2521 gcgcccggag cggtcccgcc agacgagccg gctgaggtcc cgctgcttcc cggtcaggaa
     2581 gacgaggacg cccgcctcgc tcgccgagaa gacgccgtag gcgaagcgtc gattgaagag
     2641 gaggtctccg acgagcactt tcggctcgcc cgagagcgcc cggctcttcg cgtcgaacgg
     2701 ctgggccacg agcgcgccgt cgcgaacgaa gaggaggtga ccgtccgcgt agaccgcgtt
     2761 cgtggcgccc tcgaggagcc gccgcgtcgg ccccgtctcg ccgagcttcg cgagcaggac
     2821 gccgccggcg accgagccga cgcgccccga gcggtcgagg aagacgaagt gctcgccgtc
     2881 cggcagaaac tgcgggaacc ggtgcgtggc ggtgccgagc gcctcccgcg agcccgtgac
     2941 ctcgcggacc tctccgcccc tctcggagat cgcgaggagc gggccggtcg cgaaaggcgc
     3001 gaacaggatc gtcccgtcgg ggctccaggt gccgccccgc gaatcgcgcg cgggggcgag
     3061 gcgggcgacg gcccccccgt ccgccggggt cgtcgcgagg tggccccagg tgaagaagcc
     3121 gatcctccgg ccgtcgggcg accagaaggg ccggtagccc tcctcggtgc cgggaagcgg
     3181 gcgcgcgacc gcggtcgcga ggtcgtggac ccagatccgc atcgggccgg atccttcgcg
     3241 ggctccgaag gcgacgcgac gtccgtcggg ggagacggcc ggcatcccgg cgcgctcacc
     3301 gtccagctcg atcgagacgc cggccgggag cggaagcgtg gctcggatga cgcgcggaac
     3361 gacgggctcg tcggagcccc gcccgaggaa gaggccgccc gccgcgacga gcgcgaccgc
     3421 ggcgaagccc cgggcgacga gctcgcgtcg acgatctcgc gggggcggcg ccgcggcgac
     3481 ggcgggcgcc tcgtccgcga cgcccgcctc gaggtcgacg aggacgagcc gcgcgtcggc
     3541 cacgtcgtgg agacggttcc tggggttccg ctcgaggcag cggcggagga gctgcttcag
     3601 ctgcgcgggc gtcgatgcgg gcagcgcgga gagctcgatc ggccccttca ggacgctcgc
     3661 gagactctcg gcgacggaat cgccctcgaa gagccgccgc cccgcgagca tctcccacac
     3721 gaggacgccg aacgcccaga cgtcggcgcg ccgatccacc gcgagcccct tcgcctgctc
     3781 gggcgccatg tacgccgcgg tgccgaggat cacgccgacc gccgtccccg ccgaggtcag
     3841 ggtcggcgag ttcatcaggg tcggcgactc ggtcgccgcc gacggaccga ccgcctcgcc
     3901 tcccgaggcc gcgcgcgggt ccagcgcctt cgcgaggccg aagtcgagga ccttgaccgt
     3961 gccatccggt ccgagcttca cgttctgggg cttgaggtcg cggtggacga tccccttctc
     4021 gtgcgccgcc tccagggcct ctgcgacctg gcgcgcgatc gggagcgcct cctcgagggg
     4081 aatccgcccg cgcttcatcc tctcggccag gtcctctccc tcggcgagct ccatgacgag
     4141 gacgtgcgcc gtcgttccgt cggggagcgg cgtggcctcg aatccgtaca ggtgcgcgat
     4201 tccggggtgg ttgagggagg cgaggagccg cgcttcccgc tcgaaccggg cgaggcgttc
     4261 cggctcggcg gcgaaggccg gcgggaggag cttgagcgcg agctcgcgtc cgagcctcgt
     4321 gtcgcgcgcg cgccagacgt cgcccatccc cccctcgccg agatgggcga gcacttcgta
     4381 cgggcccagc cgcgctccgg cgtcgacttt catcggaccg gcggattcta gggacagccg
     4441 gctccggaac ggcaatctcc atgcagccgg cggcggcgga ccggcgctcg aaggccgctc
     4501 ccggggaccc ttcgccgggc cggccgggac gcgggaagcc ctcgcgggcc cgggctggcg
     4561 cccgcggtcc cgtctcgtgg agaatgcgcg gccgtggggt cgcgcatcct cttcgtcctg
     4621 cccggctggc cgaaagggcg tctctggggc gagatgtcgt tccgcttccc gagcctctcg
     4681 ctcgccgtcg tcgccgcggc gaccccggag aagtgggaga ccgccctcca cgacgagggc
     4741 ttcgggccgg tcgacctcga cgacccggcc ggggtcgtcg ccctcaccgg gatgacggcc
     4801 caggcgaccc gggcctacga gatcgccgac gcgttccgcg cccgcggcaa gacggtcgtc
     4861 atggggggct tccacgcgag caacctgccg gccgaggcgc tccagcacgt cgatgccgtc
     4921 gtcgtcggcg aggccgagtc ggtctggccg aggctccttt cggacctcga ggaggggcgc
     4981 ctgcagaagg tctaccggcc cgacgggccg gtcgacacgg cggcgatccc ggtcgcgcgg
     5041 cgatccatct tcgacggcaa gggctacctc ctgacgaaca cgatccagac gacgcgcggc
     5101 tgcccttacg actgcgagtt ctgctcggtg acggcctact tcggccggaa gtaccggaag
     5161 cggccggtcg agacggtcct cgcggagctc gcggagatga agaagcgcgg ctcctacgcc
     5221 ttcttcgtcg acgacaacat cgtcgtcgac cgcggctact cgctgaagct cttccgcggc
     5281 atgaagggcc tcgggatgaa gtggctctcg cacgcctcgc tcgacctcgc ggaggacccc
     5341 gagctgctca cggcgatggg ggaagccggc tgcatcgggc tcttcgtcgg cttcgagtcg
     5401 ctcgacgaga aggcgctcgc ggcgatgggg aagaagacga accacgtggc cacctacctc
     5461 gacgcggtga aggcgttccg ggattccggg atcggcatcc tcggctcctt cgtcctcggc
     5521 ctcgacggcg acggccccga cgtcttcgag cggacgctcc gcttctgcga gagcgcgcgc
     5581 ctgccggcgg cgatcttccc gatcctcacg ccgtaccccg ggacgaaggt ccgcgaacgc
     5641 ctcctcgccg aggaccggat cctctcgagc gactggcgga actacgacat ggagcacgtc
     5701 aacttccggc cgaaggggat gacggtcgac gagctccagg cgggctacga cctcctgatg
     5761 aaggagttct actcgttcgg gtccatgtgg aagcggctcg gctggcaccg gtcgctcccg
     5821 gcgttcgggc cgatgaacct cggcttccgg agcgccctga agcgcaaggt ggccgatgcc
     5881 tgagaggtgc ggcggaaggg cgttcccggc gtgaaggtcc tgatgatctc ggcgaaccgg
     5941 gagagggacc cttctcccgt cttcccgctc ggcctcgcct acctcgcggg ccccgtcgag
     6001 cgggcggggc acgagacccg cctcctcgac ctctgcttcg ccgcggacgt cccggaggcg
     6061 gtccgcgggg cggtcgagga gatgcgcccc gacgtcgtcc tcgtctcgat ccggaacctc
     6121 gacaacgtca cctggccggt ctgccgctcc tacgtcgagg gcgtgcgcga ggtggtcgcg
     6181 ctctgccgcg acgcggcccc ggtcgtcctc ggcggctccg cgttctcgct ggagccggag
     6241 tcgttcctcg aggcgacggg cgcgcgctgt ggcgtcgtcg gcgaagggga ggaggtcgtc
     6301 gggccgctcc tcgagaggat cgcggcgggg ctcccgctcg cgggactccc cggcgtcgtc
     6361 gagccgggcg gcgacgggtg gacggcgccg cgcacgccgg cgaccgtcgg cgcgccgagc
     6421 cgggggagct tcgatgtctc gcgctacctg acggaggggg gcgccgcgaa cgtccagacg
     6481 aagcggggct gccccttcac ctgcgtctac tgcacgtacc cggtcctcga ggggagccgc
     6541 gtgcgcaccc gcccggcggc ggaggtcgtc cgcgagctcc ggacgctcgt cgacgagcac
     6601 ggcgtcgact acgtcgagtt ctgcgacgac atcttcaact tccccgaggc ggccgcccgc
     6661 gagctctgcg aggcgatgat cgcggacggc ctgcgcctct cctggtcggc gttcgtcaac
     6721 ccggggcacc tcgacgcccg cctcgtcgac ctgatggccc gggccgggtg cgacgccgtg
     6781 gagatcggca ccgactccgg ctcgccggcg atgctccgga gcctcgggaa gtcgttcacg
     6841 gtcgaggacg tgcgcgcggc ctcgcgcctc tgccgcgacg ccgggctcgc caccgcccac
     6901 tacctcctct tcggcgggcc cggcgaggac gacgccacgg tcgccgagag cgtcgcgctg
     6961 atggacgagc tcgcgcccga ggccgtcatc gtcatggtcg gcatccgcgt ctacgccggg
     7021 accgacctcc acgcgaccgc cgtgcgcgaa ggggtcgtcc gggccgacca gtgcctcctc
     7081 gagccggtct tctatcccga cgagtcgcgg ctccggaaga tcgcggagcg cgtggcggag
     7141 ctggccgcgg cgcggcggac ctggatcgtc ccggggctcg acgtgaacac ctcctccggc
     7201 gtcctcgaga tgctccgccg ccgccacccg cgcgggccgc tctggaaggt cctcggccag
     7261 cgggagagct ggcgggggcg gcggcgcgct gctggcgaaa ccggtccccg cgccgtctga
     7321 gcggccaacg cccattcgga cacgacgccg tccggacggc cgggcgatcc cgccctcgcg
     7381 tcagcgctcg gcgcccgcct tgatcccgag ggccgggtcg tagaggttct ccgcgctcgt
     7441 gccggtccga gtcgcgagga gccggacgaa tccgatcgtc ctcccggcgt ccttctcgag
     7501 cgcctcggcg caggagggga agatcccctt ccccgcggcc tcgatcgcgc gctcgaagtc
     7561 ggcccagtgc ctccagccgt cgggctgcag ctcggcggag acgtccgtca ggcagggcga
     7621 gccgcgccag atctccgtcc acgcctccgc ggtcaggaac gcgcggcagc cctcctccca
     7681 gaacgcctgc ccgttcgact gcggctcggt gagccacggc gggactccgt ccgaaagcgg
     7741 ccggacgacg gcgggcacga cgacgccgag cctcccgccg ggccggagga agcgggtgag
     7801 gtagtcgagg tagaggaggt cggtgccgaa gtactggtag gcgtcgatcg agacgatcgc
     7861 gtcgaagtag gcggccgcga aggggagcgt gtgggcctcg gcgcgcaccg ggcagacgag
     7921 gtcggcggcg ccggccgcgg cgaccctctc ccagttcttg tcggggctca tccagaggtc
     7981 gacggcgtgg accttcgcgc cgtactcctt cgcgaggaag accgacgtca tcgccttccc
     8041 gcagccgagg tcgaggaccc gcgcgccggg agcgagcggg aacctctcgc agagccactc
     8101 gagcagccag atcgcgcacg gccccatctg gttgtcgagg aggaactcgg cgtcgtagcg
     8161 ggcggagcgg gggaactcgg ggcgcgcgag gtcgacgggg gcgtgaggca tgtgggctcc
     8221 ttcggacggt gcctcggaca ttctgccgcg atccaccgcg gccgggggcc gcgttagtct
     8281 cggcaccctc cggaggcgac gatggccgag ccgaagacga agaagaccga cgcgagcgtc
     8341 gaggcgttcc tggatgcggt cgcggacccg gggagacgcg aggacgcccg ggctgtcctc
     8401 gcgctgatga agcggatcac gggggaggag ccgaagatgt ggggcccgag catcgtcggc
     8461 ttcggctcgt acacatacac ctacgcgagc ggccggaccg gcgactggcc gatcgccggg
     8521 ttttcgccac gcaagcaggc gctcacgctc tacgtcatgc ccgggttcga gaaggacgcg
     8581 gacctgatga agcgtctcgg gaagcacaag acgggaaagt cgtgcctcta cctgaaccgt
     8641 ctcgcggacg tcgacgcggg cgtcctcgag gagctcgtcc ggcggtcggt ggagcggatg
     8701 aggaaccgga gagcctgaag ggacggatac gacggggcgt ccggggtccc tccctgcgtg
     8761 cggccggagg tcaacggacc cggatccgcc ccgtccccgc ccccgcgatc cgcccgatcc
     8821 ggcgcgcgtc cgcaacgccg cgggcgcgaa gtgacgacag gagcgcctcg cagcgcttct
     8881 cgggcagcgc gacgaggagc ccgccggagg tctgcgcgtc ggcgaggagg aggcggtcga
     8941 cgcgcgagag gccgggcgcg aagtcgacgt ggggcgcgac gtgctcgagg ttctcgagcg
     9001 agccgccggg gacgccgtcg gccgcggcga gctcgcgcac gtcggggagg agcgggacgg
     9061 aagccgcctc gatctccgcg tcgacgccgg aggcggccgc catctcgcgg aggtggccga
     9121 ggaggccgaa gccggtcacg tcggtgcagg ccgaggcgcc ggcctcgacc atcgcctcgg
     9181 cggcggcgcg gttcagggtc cgcatcgtcg tcacgaggcg acgccccgcc tcctcgcccg
     9241 cgaggccgcg cttcaccgcc gtggcgagga ctccggtgcc gatcggcttc gtcaggacga
     9301 gagcgtcgcc cgggcgtgcg ccggcgttcg tcacgacccg gtcggggcgg acgacgccgg
     9361 tcaccgcgag gccgaacttc ggctcggtgt cgtcgacggt gtgcccgccg aggatctcga
     9421 tccctgcctc gcgcgccacc tcggccgccc cggcgaggat ccgctcgagg acggagaggg
     9481 ggaggcggcg cgacggaaag gcgacga
//
LOCUS       DAOTEZ010000142         9507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5097_length_9507_cov_4.548138, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000142 DAOTEZ010000000
VERSION     DAOTEZ010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9507
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5097_length_9507_cov_4.548138"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /locus_tag="P5164_09010"
     CDS             <1..1166
                     /locus_tag="P5164_09010"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HRY44071.1"
                     /translation="RDRGAVWVAVRIPDGHVSAHANHSRITTFPLENGTTSISSKSFE
                     RLSDPRVEVVYAHDVVDVARRKGYFTGRDEEFRFADAYAPIDFGAARFCEARVWSFFN
                     GLCGGMDAWLDHAKGKDLAKRLPLSVKPDRKVSVADLMAAKRDHFDGTELDMRRDAGA
                     GPFGLPYRWRPLTFEHEGKTYLNERATATQQTGFSYVAQMRRWLPSPIGGILWFGVDD
                     AATSVYMPISCGITRVPSSLAVGNGDLLAFSDTSAFWTFNKVANFAYLRYDRMSADVR
                     RVQQELETKFLGQVAAIDAAALALHEKDPSRARELLTDRASAVAAETVGRWEELFRYL
                     LVKYMDGNVKKERGGRFERNEWGFPKPPDHPGYPDSWKRRVVEDAGERLLHPK"
     gene            complement(1203..1379)
                     /locus_tag="P5164_09015"
     CDS             complement(1203..1379)
                     /locus_tag="P5164_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44072.1"
                     /translation="MIRATVTLLPRISALENVEMPPAFGGLPLKVRRAKVSKLLGKVG
                     LGDRMDHEPRRRAA"
     gene            complement(1390..3693)
                     /locus_tag="P5164_09020"
     CDS             complement(1390..3693)
                     /locus_tag="P5164_09020"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HRY44073.1"
                     /translation="MVTIYVIAPLLLLAVVLAAVWLDRFSVPVILVALGLGLLAGSDV
                     LGLWHFDDVDLANQVANAGLVFILFHGGLVTKGSVLRTVALPAGGMATWGVVLTAAAM
                     FGILHLGLGWAFERSLLLAAVISSTDAAATFSILRRQSLRPRLASTIEVESAANDPMA
                     ILLTLVVVEALTAGNGLGWTILPVFAWKFVAGPVLGWLLARVALAIFDRLNPQDRGYY
                     YVLLLAVVLLTYGLAEAAKASGMLAVFTAGLVMGNRKFIYQQGIRNFSAALSMIANVS
                     LFVMMGLLVFPRQWGGLWLHGIVLFLVLTFAARPAAVWLGTLGMGFRAKERHFMCWAG
                     LRGAVPIVLATYPMAAGIPAGQDVFNLVFFAVLLSVGIQGSTLGPMARFLGLATPSRP
                     QPRYGLDLVTMAHSDLDLVVVDLPGPKGRPGPRIRELALPPGAILVLVTRDDEVVAPT
                     SNTRLLGWDQVTVLAHSGDEADVRSALLEPFGRADAGEEPGTRAMGPGPSEPAGAGDD
                     GPLRDHVVLLGHGEVGSVLARFLRLREVPFVVIEKDDTTATALSGQGVRVLRGRGEDP
                     DLLARAGIEDARMLLVTATRPVSARRAIEHARRMNPRLEVIARVHHESLRSALSALPR
                     TWVVQGDVELAYAMARSMLLASGMSAIETEALIVDARRGEPGTTPRRFIEIPVPPASP
                     AVGQRLADLTLPPGCLVVEILRAGEVIVPGGTTAIHAEDVLLVLANIGQARTIERLVH
                     PDAPAADGSADSSPDAAGPPRDLAPAS"
     gene            complement(3867..4082)
                     /locus_tag="P5164_09025"
     CDS             complement(3867..4082)
                     /locus_tag="P5164_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44074.1"
                     /translation="MDPDIVLADEPTGNLDTTSGTDIMGVFLDLWKQGSTLVVITHDM
                     ALARRASRIVEIRDGRIVSDRPAEQAA"
     gene            complement(4118..>9507)
                     /locus_tag="P5164_09030"
     CDS             complement(4118..>9507)
                     /locus_tag="P5164_09030"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRY44075.1"
                     /translation="LPKGASREEYRRRLAKLRDEKRNEAVHPPEERCSREEMRAPGRA
                     PRILLTNVKQLELLLTRQKDVELFAGSRLEILVVDEAHTFSGANGAETACLLRRLRTF
                     CGTGERETVCVATSATLADRGASDDPARTFASRFFGVPEESVTTVTEEYEEPPFEAER
                     LVPATIPDPARALPAILAALEGADGAAIARAIGGGLELAGGSWREALGESLARNELLF
                     QLSEQLRRPALLSAVAEKVAEACDRPVAEEEVLAWLALGAAARRDGRPFVRPVVHDFV
                     RGIDGAVVSFPEGTMPRLWLSAREEAAAEGGQELVRLPVLTCTTCGEHYFSHSVADLD
                     ANGAVPGGGEAAEQGTFWGTLSAEAGGRRLLLTDRVISVDEEENGDEGPDLPKVFFCR
                     HCGTLASAEGGACPACGRPGAPVPLFLVRQKEEAPGHLSRCVSCGAAGRRRGGGYREP
                     ARSVRAVTVSDVHVLAQDMVRQLSPRRLLVFADSRQDAAFQAGWMRDHSRRFRLRALM
                     WEELQRGTKTVIDLTAALDRLMEADDELSQALLPEVWAETRKDAGFEHRERRKFFLRV
                     QVLREVTTGLKQRIGLEPWGRLRVDYDGLSAATPGLAALAASHGLDAEALAEGIGGLL
                     DRTRRRMILLDKDKRIFSRFWRDGDPDVLRGFVPAFQGVPTGLKLRREAGDDKNRVSA
                     WVNDQRNTAVLQVVRRWGVPKEHAAPVVEELWKLLAERTKLLSPAVLTGSRGNALPGA
                     SGTFQVDADRLLLNPSRGLWRCSECRRAQVRRSPGDVCPSWHCHGTLAREDEPADDYD
                     LSLLDGGVPMLRPREHSAQVPASERERLERLFKDETTEAVNTLVATPTLEMGVDIGAL
                     DAILLRNVPPSPANYWQRAGRAGRRERMAVALTYAGARGHDRSAFAEPLRLLAGSVTP
                     PQFNLRNELLVARHVHAAALTRLHSLAREGGAAGEALAATLELAFPAEVRSWLFDAEG
                     RVRREPFDLSGFRGEVAARADDLAGYVSSVFRGRWPEADAAVVAEERVARLVGEMPDR
                     LEDVLKTLRKRLDWALAMMARLDRERAERGVLERDEESIHDRCKALVRKLKGESRRAR
                     GGTEGYDDANTFGVLAAEGFLPGYGLETGSIQGIPVFPRGGAGMEDFSLPRPPAVALR
                     EYVPGNLIYANGNRFVARLFHFEAEERPAYFLVDAEREAVEEVSAPGAAPAGGAVAPV
                     AAVTAPLRAAAVTDVELVHTSQITDEEDFRFQLAVSIFALERDEHGGGRAFCWGPREV
                     QHRRAARLRLVNVGVKNLVTKKDEFGFPVCTVCGQSRSPFASREELQEFREKHQQRCR
                     QPVEPTGFYADLVADVLRLPAGASRREAFSVLEALRAAAAQTLEMELEDLQVVVVGRR
                     GEETVDGLLYDPMPGGSGLLDQLLERFPEVVAKAVSIARDCPAGCGGSCPDCLQVYRN
                     AAYHGHLDRALVVEKLTEWGEALTLSHEIPPKQPAARPSGERAPSNAAEDTLRELLHR
                     AGFPEAQWQKRIDLGRPLGGTVPDAFYSLDDEDEPGLCIYVDGLSASLHGDPERRRMD
                     RVLREELQARGYVVIEIPASDLADREAVARAFSRIARKLVGSERAQSLRTDTSWFESG
                     GEPAAVGPVVVPFRRVEAAVSEQFRTCVPLVELAAAAGGWGEAGPAGDDEWVEPTTTR
                     KLRKGMFVAQVKGSSMEPLVADGCWCLFASPVEGSRTGRTLLVQLRDPGDPEDGGRLT
                     LKRFESREVAGKDSPEPGAERSGRVVLRPRNESVDAMGRRQFSPLEVEDDFEGSVRVI
                     AELLEVLPSGA"
BASE COUNT         1379 a         3588 c         3272 g         1268 t
ORIGIN      
        1 accgcgaccg cggcgccgtc tgggtcgccg tccggatccc cgacggccac gtctccgccc
       61 acgcgaacca ctcccggatc acgacgttcc cccttgagaa cgggacgacc tcgatcagct
      121 cgaagagctt cgagaggctc tccgacccgc gggtcgaggt cgtctacgcc cacgacgtcg
      181 tcgacgtcgc gcgccgcaag ggctacttca ccgggcggga cgaggagttc cgcttcgccg
      241 acgcctacgc cccgatcgac ttcggcgccg cccgcttctg cgaggcgcgc gtctggagct
      301 tcttcaacgg gctctgcggc gggatggacg cctggctgga ccacgcgaag gggaaagacc
      361 tcgcgaagcg cctcccgctc tcggtgaagc ccgaccggaa ggtctccgtc gccgacctga
      421 tggccgccaa gcgggaccac ttcgacggca ccgagctcga catgcggcgc gacgccggcg
      481 ccgggccgtt cgggctcccc taccgctggc ggccgctcac gttcgagcac gaggggaaga
      541 cgtacctcaa cgagcgggcc accgcgacgc agcagaccgg tttctcgtac gtcgcccaga
      601 tgcggcgctg gcttccctcc cctatcggcg gaatcctctg gttcggcgtc gacgacgccg
      661 cgaccagcgt ctacatgccg atctcgtgcg ggatcacccg cgtcccctcg agcctcgccg
      721 tcgggaacgg cgacctcctg gccttctccg acacctcggc cttctggacg ttcaacaagg
      781 tcgcgaactt cgcctacctt cgctacgacc ggatgagcgc cgacgtccgg cgcgtccagc
      841 aggagctgga gacgaagttc ctcgggcagg tcgcggcgat cgacgccgcg gccctcgcac
      901 tccacgagaa ggacccttcc cgcgcccgcg agctcctcac ggaccgggcc tcggccgtcg
      961 ccgcggagac ggtcggccgc tgggaggagc tcttccgcta cctcctcgtc aagtacatgg
     1021 acggcaacgt gaagaaggag cgcggcgggc ggttcgagcg gaacgagtgg ggcttcccga
     1081 agccgcccga ccacccgggc tacccggact cctggaagcg ccgggtcgtg gaggacgccg
     1141 gggagaggct gctccacccg aagtgaccgc ccccgtcctg cggggagccc attccctctc
     1201 ccctacgccg cccgcctcct cggctcgtgg tccatgcggt cgccgaggcc gaccttcccg
     1261 aggagcttcg acactttcgc gcggcggacc ttcagcggca aaccgccgaa ggcgggcggc
     1321 atctcgacgt tctcgagcgc gctgatccga ggcagcagcg tgacggtcgc ccggatcatt
     1381 ccgtgatcgt caggaagcgg gggcgagatc gcgtggaggc cctgctgcgt cgggcgagct
     1441 gtcggcggag ccgtcggccg cgggcgcgtc cggatggacc aggcgctcga ttgtccgcgc
     1501 ctggccgatg ttcgctagca cgagcagcac gtcctccgcg tggatcgcgg tcgttcctcc
     1561 cggcacgatc acctcgccgg cccgcaggat ctcgacgacg agacacccgg gcgggagggt
     1621 gagatccgcg agccgctggc cgacggcggg ggacgccggc gggacgggga tctcgatgaa
     1681 ccgccttggc gtggtgcccg gctcccctcg ccgcgcgtcg acgatcagcg cctcggtctc
     1741 gatggcgctc attccggacg cgagcagcat ggacctggcc atggcgtacg cgagctcgac
     1801 gtcgccctgc acgacccacg tccgcggaag ggccgacagg gcgctccgca gcgactcgtg
     1861 gtggacgcgg gcgatcacct cgagccgcgg gttcatccgg cgcgcgtgct cgatcgcccg
     1921 gcgagcggac acgggccggg tcgcggtgac gaggagcatt cgcgcgtcct cgatgccggc
     1981 ccgggccagg aggtccgggt cctcgccacg gcctcgcagg acacgcaccc cctgaccact
     2041 cagagccgtc gcggtcgtgt cgtccttctc gatcacgacg aacgggacct cacgcagccg
     2101 caggaaacgg gcgagcacgc tccccacctc gccgtgaccg aggagcacga cgtgatcgcg
     2161 caacggcccg tcgtctccgg ccccggcggg ctcggacggc ccggggccca tcgcgcgcgt
     2221 cccgggctcc tctcccgcgt cggcccggcc gaacggctcg agcagcgccg accggacgtc
     2281 ggcctcgtcc ccgctgtgcg cgaggaccgt gacctgatcc cagccgagca gcctcgtgtt
     2341 gctggtggga gcgacgacct cgtcgtctcg tgtgaccagg acgaggatcg cgccgggagg
     2401 cagggcaagc tcccggattc gtggtccggg tcgacccttc gggcccggca ggtcgaccac
     2461 gaccaggtcg agatcgctgt gcgccatggt cacgaggtcg aggccgtagc gcggctgggg
     2521 ccgcgacggc gtggccagcc cgagaaatct cgccatcgga ccgagcgtcg agccctggat
     2581 ccccaccgac aggaggaccg cgaagaagac gaggttgaac acgtcctgcc ccgcagggat
     2641 gccggccgcc atcgggtagg tcgcgaggac gatcgggacc gcaccgcgaa ggcccgccca
     2701 gcacatgaag tggcgttcct tcgccctgaa acccatcccg agcgtcccca gccacacggc
     2761 ggcgggccgg gcggcgaacg tcaggacgag gaacagcacg atgccgtgga gccacaggcc
     2821 gccccactgt ctcggaaaga ccaggagccc catcatcacg aacagggaga cgttcgcgat
     2881 catcgacaga gccgccgaga agttccggat accctgctgg tagatgaact tccggttccc
     2941 catgaccagg cccgccgtga agaccgcgag catcccgctg gccttcgccg cttccgccag
     3001 gccgtacgtc agcagcacga cggcgagcag aagcacgtag tagtagcctc gatcctgcgg
     3061 gttcagccgg tcgaagatcg cgagcgcgac gcgcgcgagc agccagccca gcaccggccc
     3121 ggcgacgaac ttccacgcga agacgggcag gatcgtccag cccaggccgt tgccggcggt
     3181 cagcgcctcg acgacgacga gcgtgaggag gatcgccatg gggtcgttcg ccgcgctctc
     3241 gacctcgatc gtcgaagcga ggcgcggccg gagggactgg cgacggagga tcgagaaggt
     3301 cgcggccgcg tccgtcgacg agatcacggc ggcgaggagg agcgagcgtt cgaaggccca
     3361 gcccaggccg agatgaagga tgccgaacat cgcggccgcc gtgagcacca cgccccaggt
     3421 cgccattccg cccgccggga gcgcgaccgt acgaaggacg ctgcctttcg tgacgagccc
     3481 gccgtggaac aggatgaaca ccaggcccgc gttcgcaacc tggttcgcca ggtcgacgtc
     3541 gtcaaagtgc cacagcccga gaacgtcgct gccggccagc agtccgagcc ccagagccac
     3601 gaggatgacg ggcacgctga accgatcgag ccacacggcc gccaggacga cagccaggag
     3661 cagcagcgga gcgatgacgt agatggtcac catcaggatt tcatccggtc cacgcgaacg
     3721 gcccggcggc ggcggagccc gctcggaagg aacccgtttg acggttccga gaaggatcct
     3781 agcatcgcga tcgttcccgg aatccgctcg cggaacggag cgcggacgct cggcgaaagg
     3841 gctgcgacgt ctcgcggggc gacgccctac gccgcctgct ccgccgggcg gtcgctgacg
     3901 atccgtccgt cgcggatctc gacgatgcgg ctcgcgcggc gggcgagcgc catgtcgtgg
     3961 gtgatcacga cgagcgtcga gccctgcttc cagaggtcga ggaagacccc catgatgtcg
     4021 gtgccggagg tcgtgtcgag gttcccggtc ggctcgtcgg caaggacgat gtcggggtcc
     4081 atcgcccgcg atggatcccg acatcctcgc gatgggttca cgcccccgac ggcagcacct
     4141 cgagcagctc cgcgatcacc cgtacgctcc cctcgaagtc gtcctcgacc tcgagcggcg
     4201 agaactggcg ccggcccatc gcatccacgc tctcgttccg aggccggagt acgacccttc
     4261 cgctccgttc ggcgcccggc tcgggagagt ccttcccggc gacctcgcgg ctctcgaacc
     4321 gtttcagggt cagccgtccg ccgtcttccg gatcgcccgg gtcgcgaagc tgcacgagga
     4381 gcgtccggcc ggtccggctc ccctcgacgg ggctcgcgaa gaggcaccag cagccgtccg
     4441 cgacgagcgg ctccatcgaa ctccccttca cctgcgcgac gaacatcccc ttgcgcagct
     4501 tgcgggtcgt cgtcggctcg acccactcgt cgtcgccggc cggccccgcc tcgccccagc
     4561 ccccggcggc cgcggccagc tcgacgaggg gcacgcacgt ccggaactgc tccgacaccg
     4621 cagcctccac gcgacggaac gggacgacga cgggcccgac cgccgccggc tcgcccccgc
     4681 tctcgaacca cgacgtgtcc gtgcgcagcg actgcgcccg ctccgagccg acgagctttc
     4741 gggcgatccg gctgaaggct cgcgccacgg cttcgcgatc cgcgaggtcc gacgccggta
     4801 tctcgatcac gacgtagccg cgggcctgga gctcctcgcg aaggacgcgg tccatccgcc
     4861 gacgctccgg gtcaccgtgc agggaggcgc tcaggccgtc gacgtagatg cagaggcccg
     4921 gctcgtcctc gtcgtcgagg gaatagaagg cgtccggcac cgtgcccccg agcggccgcc
     4981 cgaggtcgat ccgtttctgc cactgggcct cggggaaccc ggcgcggtgg agcagctcgc
     5041 gcagcgtgtc ttccgccgcg ttcgacggcg cgcgctcgcc cgacggccgt gcggccggct
     5101 gcttgggcgg aatctcgtgg gagagcgtca gcgcctcgcc ccactccgtc agcttctcga
     5161 cgacgagcgc ccggtcgagg tgcccgtggt aggccgcgtt ccggtagacc tgcaggcagt
     5221 cggggcagct tcctccgcag cccgcggggc agtcgcgggc gatcgacacc gccttcgcga
     5281 cgacctcggg gaagcgctcg aggagctggt cgagaaggcc cgagcctccc ggcatcgggt
     5341 cgtagaggag gccgtcgacc gtctcctccc cgcgccgtcc gacgacgacg acctgcaggt
     5401 cttcgagttc catctcgagc gtctgcgccg cggccgcccg gagcgcctcg aggaccgaga
     5461 aggcctccct ccggctcgcg cccgccggca ggcggaggac gtcggcgacg aggtcggcat
     5521 agaagccggt cggctccacc ggctgccggc agcgctgctg gtgcttctcc cggaactcct
     5581 gaagctcctc gcgcgaggcg aacggagagc ggctctggcc gcagacggtg cagaccggga
     5641 agccgaattc gtccttcttc gtgacgaggt tcttcacgcc gacgttcacg aggcgcagcc
     5701 gcgcggcccg ccggtgctgg acctcgcgcg gcccccagca gaacgcccgg cccccgccgt
     5761 gctcgtcccg ctcgagggcg aagatcgaga ccgcgagctg gaagcggaag tcctcctcgt
     5821 cggtgatctg ggaggtgtgg acgagctcca cgtccgtcac cgccgcggcg cgcagcggag
     5881 cggtgaccgc ggcgaccggc gcgaccgctc cgcccgcggg cgcggcgccg ggggccgaaa
     5941 cctcctcgac ggcctcccgc tcggcgtcga cgaggaagta cgccggacgc tcctccgcct
     6001 cgaagtggaa gaggcgcgcg acgaagcggt tgccgttcgc gtagatgagg ttcccgggga
     6061 cgtactcgcg cagcgcgacc gcgggcggcc tcggcaggga gaagtcctcc atccccgcgc
     6121 cgccgcgagg gaagaccggg atcccctgga tcgagccggt ctcgagcccg taacccggga
     6181 ggaagccctc cgcggcgagg acgccgaacg tgttcgcgtc gtcgtacccc tccgtcccgc
     6241 cccgcgcgcg gcgcgactcc cccttgagct tcctcacgag cgccttgcag cggtcgtgga
     6301 tcgactcctc gtccctctcg agcactcccc gctccgcccg ttcgcggtcg aggcgcgcca
     6361 tcatcgcgag ggcccagtcg agccgcttcc ggagcgtctt caatacgtcc tccagccggt
     6421 cgggcatctc cccgacgagc cgggcgacgc gctcctccgc cacgaccgcg gcgtcggcct
     6481 cgggccagcg cccacggaag accgacgaga cgtacccggc gagatcgtcc gcgcgcgcgg
     6541 cgacctcccc tcggaacccg gagagatcga acggctcccg ccggaccctc ccttccgcgt
     6601 cgaagagcca gcttcgaacc tccgccggga acgccaactc cagcgtggcc gcgagcgctt
     6661 cgccggcggc tccgccttcc cgcgcgagcg agtggagccg cgtcagggcc gcggcgtgga
     6721 cgtgccgcgc gaccagcagc tcgttccgca ggttgaactg cgggggcgtg accgatcccg
     6781 cgaggaggcg gagcggctcg gcgaacgcgc tccggtcgtg tccgcgcgcg ccggcgtacg
     6841 tcagcgcgac ggccatccgc tcgcgccgcc ccgcgcggcc cgcccgctgc cagtagttcg
     6901 ccggcgaagg cggaacgttc cggaggagga tcgcgtcgag ggcgccgatg tcgacgccca
     6961 tctccagcgt cggcgtcgcg acgagagtgt tcaccgcctc ggtcgtctcg tccttgaaga
     7021 gccgttccag ccgctcgcgc tctgacgccg gcacctgggc cgagtgctcc cgcgggcgga
     7081 gcatcggcac gcccccgtcg aggagcgaga ggtcgtagtc gtcggccggc tcgtcctcgc
     7141 gcgcgagcgt cccgtggcag tgccacgaag ggcagacgtc tcccggcgag cggcgcacct
     7201 gcgccctgcg gcactcggag cagcgccaga gtccgcggct cgggttcagg aggagccggt
     7261 ccgcgtcgac ctggaacgtc ccgctcgcgc cggggagcgc gtttccgcgg ctcccggtca
     7321 ggacggcggg agagaggagt ttcgtccgct cggcgaggag cttccaaagc tcctccacga
     7381 ccggggcggc gtgctccttc ggcacgcccc agcggcgcac gacctggaga acggccgtgt
     7441 tgcgctggtc gttgacccac gcggagaccc ggttcttgtc gtcgcccgcc tcccgccgga
     7501 gcttcagccc cgtcggcacg ccctggaacg ccggcacgaa cccgcgcagg acgtcggggt
     7561 ccccgtccct ccagaacctc gagaagatcc tcttgtcctt gtcgaggagg atcatccgcc
     7621 gccgcgtccg gtcgaggagg ccgccgatcc cctccgcgag cgcctccgcg tcgaggccgt
     7681 ggctcgccgc gagggcggcg aggccgggcg tcgcggcgga gagtccgtcg tagtcgaccc
     7741 tcaaccgacc ccacggctcg aggccgatcc gctgcttcag gcccgtcgtc acctcgcgga
     7801 ggacctgcac gcggaggaag aacttccgcc tctcgcggtg ctcgaacccc gcgtccttcc
     7861 gcgtctccgc ccagacctcc gggaggagcg cctgcgaaag ctcgtcgtcc gcctccatca
     7921 gccggtcgag ggcggccgtc aggtcgatca ccgtcttcgt cccgcgctga agctcctccc
     7981 acatcagggc gcgcagccgg aaccgcctcg agtggtcccg catccagccc gcctggaagg
     8041 ccgcgtcctg ccggctgtcg gcgaagacga ggagcctccg cggcgagagc tgccggacca
     8101 tgtcctgcgc gaggacgtgg acgtcggaga ccgtcaccgc gcggacggag cgggcgggct
     8161 cgcggtagcc gccccccctg cgccggcccg ccgcgccaca cgagacgcag cgcgagaggt
     8221 gcccgggggc ctcctccttc tgcctcacga ggaagagcgg caccggcgcc cccggccggc
     8281 cgcaggccgg gcaggcgccc ccttcggcgg acgcgagcgt cccgcagtgc cggcagaaga
     8341 agaccttcgg caggtccggg ccctcgtccc cgttctcctc ctcgtccacc gagatgaccc
     8401 gatccgtcag gaggagcctc cgccctcccg cctccgcgga gagcgtcccc cagaacgtcc
     8461 cctgctctgc cgcctcgcct ccgccgggga cggccccgtt cgcgtcgaga tcggcgaccg
     8521 agtgggagaa gtagtgctcg ccgcacgtcg tgcaggtcag taccgggaga cggacgagct
     8581 cctgcccccc ctccgcggcg gcctcctctc gggcggagag ccagagcctc ggcatcgtcc
     8641 cctccgggaa gctgacgacg gcgccgtcga tgccgcgcac gaagtcgtgg acgacgggcc
     8701 ggacgaacgg ccgcccgtcc ctgcgggccg ccgcgccgag cgcgagccag gcgaggacct
     8761 cctcctcggc cacgggcctg tcgcacgcct ccgccacctt ctccgccact gccgagagga
     8821 gcgccgggcg ccgcagctgc tccgagagct ggaagagaag ctcgttgcgc gcgagcgatt
     8881 ctccgagcgc ctcgcgccag ctccccccag cgagctcgag cccgcccccg atcgccctcg
     8941 cgattgcggc tccgtccgcg ccctcgagcg ccgcgagaat cgccggaagc gccctcgccg
     9001 ggtccgggat cgtcgccggg acgaggcgct ccgcctcgaa cggcggctcc tcgtactcct
     9061 ccgtgacggt cgtgaccgac tcctcgggaa cgccgaagaa ccgggatgcg aacgtccggg
     9121 ccgggtcgtc gctcgccccg cgatcggcga gcgtcgccga cgtcgccacg cagaccgtct
     9181 cgcgctcccc cgttccgcag aacgtccgga ggcgccgcag gaggcaggcc gtttccgcac
     9241 cgttcgcccc cgagaacgtg tgcgcctcgt cgacgacgag gatctcgagc cgcgaaccgg
     9301 cgaagagctc gacgtccttc tgccgcgtca ggaggagctc cagctgcttg acgttcgtca
     9361 ggaggatccg cggcgcccgc cccggggccc gcatctcctc ccgcgagcag cgctcctccg
     9421 gcgggtggac cgcctcgttc cgcttctcgt cgcggagctt cgcgaggcgc cgccggtact
     9481 cctcgcgcga ggcgcccttc ggcagcc
//
LOCUS       DAOTEZ010000143         9471 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5132_length_9471_cov_4.030161, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000143 DAOTEZ010000000
VERSION     DAOTEZ010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9471
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5132_length_9471_cov_4.030161"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="P5164_09035"
     CDS             complement(<1..249)
                     /locus_tag="P5164_09035"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY44076.1"
                     /translation="MSGGEPRPMFSVVIPTFSRLDLLRRALTGWERQEPGVEFEVIVG
                     DDGSTDGTARFLASWVPTRHAFSWFSQSNAGPARARNSA"
     gene            complement(246..2156)
                     /locus_tag="P5164_09040"
     CDS             complement(246..2156)
                     /locus_tag="P5164_09040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY44077.1"
                     /translation="MSRDLDVRLRRAQEEISDLGRELLEVHQGMEKLLRLLESGAPGS
                     APAPLILEERITHLEARLRRLEEAAGRAAAGQPSLLRKVARGAWRAVRAAGKQGVRRA
                     WRTLKLRQGPTLPPAEWVFETRLVARPPVPSPAVTVLVTRESEGAVREWLGHQSLPDV
                     EVFVVPPASGEGAEPGPEPRGRWLFFAPDAPAAVSLTFLEEHLWAAAVEDLVFTAEGE
                     GRSARVVLARAALLDRQSLSLRLPDAEGSTEGRRVVGRSLPGSGAVGRVTIAVPPDTV
                     SVGGLWVRRGAWRSGLPLERQLTPLDGLLGSADTPAAATGNDGIVAVVGSRSGWGGTA
                     FARRLAEALGAEVPFVAFDADEEEPADGVAAYSAGIVLPPPLRLSALGLVARRRHAQA
                     VVDLRWRPDPLFLARSLRLRHPELRVLGLSEDGRADGIGLPCLPPAGAAAPVPSEGRI
                     AARARLAVPEGNVAVVLADTLDEDSGVFELLQAAATLEGQGGWTFLVGGNGPLRPVVS
                     DLVASRRLASLRVATEGLDDLLSAADLFCLPGTGPFRAQLLLEALARGVPALVSEPTA
                     RALDARDAVALVKERADGAALAAALVPLASATAREELARRGLDAARSWIARTRPEERW
                     REGLGGSGDSRP"
     gene            complement(2153..3409)
                     /locus_tag="P5164_09045"
     CDS             complement(2153..3409)
                     /locus_tag="P5164_09045"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY44078.1"
                     /translation="MKAAIEVRGLGLRYRLYPSAFDRLKQIVAGERKRFYREQWALRD
                     VTFDVAEGRSFGIIGPNGSGKSTLLKVVCDLLAPTEGLVRRRGRLAALLELGAGFNAE
                     YTGRENVFLNASLLGMPREQIERVYPEIVAFSQLADFMDQPVKTYSSGMFVRLAFSVA
                     AHVDPEVLVVDEALAVGDAVFQHRCMKKIADLRARGTTILLVTHDMNALSSLCDEAMW
                     IEEGRVRLVSSPREIVQRYLAWVYENRDSELRISSEVDRSGVLRYGDGRARLERWGVV
                     DETGGATTMLVPGERYEVVLDAAFHADVAEPILGFQIRDPFGRELISMNTTERGTPLP
                     PARAGERLTVRFAFPWPELAETDYSLSPAVASGTQENHTVLDWIDGDVILRSVPTSRV
                     LGLFRPHDVTLAVERRGTEVPRPEGS"
     gene            complement(3406..4254)
                     /locus_tag="P5164_09050"
     CDS             complement(3406..4254)
                     /locus_tag="P5164_09050"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY44079.1"
                     /translation="MTDVVEIRPGRVEIPSLRRFGATLREHRYLLWQLAVREFLGPFR
                     GSLLGAFWAFATPLVLLFVYAFVFSFVFRMRWGGSVSESFLSYGLVLFASFIPYSFLS
                     TALIRSSSLIVSHGGLVSKVVFPLEVLPASVLISGLLQLAVTTSILVVLAPVALGAPL
                     TGLLRALPVLALHLILTAGLTFFLSSLAVFVRDLGTVVGIALQVLFFLTPITYPESAF
                     PARFRWVQEVNPLAVVVRLWREAILFGRGPDLASLGTLAAMALVSFAAGYWWFHRTRK
                     AFVEVL"
     gene            4670..5224
                     /locus_tag="P5164_09055"
     CDS             4670..5224
                     /locus_tag="P5164_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44080.1"
                     /translation="MTSPKGLLVRLAGALSASGMPWMITGSTASAFYGEPRATQDVDV
                     VVDPTPDQVLRFLDELRDGFYVSRDEAGDALRIRGMFNVIDLESGWKAGIIVCPDRPF
                     ERNEIARRRPARLFGVDVFVISPEGSVVSKLRWAASSGSERQLRDAASVLAGCQGDID
                     LDYIRREAEVAGVSHLLAKILPPG"
     gene            5347..6510
                     /locus_tag="P5164_09060"
     CDS             5347..6510
                     /locus_tag="P5164_09060"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY44081.1"
                     /translation="MRYVSRRIEPVLRRAARDFPVVILTGPRRSGKTTVLRRVFPRAS
                     YVLLEDPDVLLRVKGDPRGFLEERKLPVILDEIQNAPELLGYVRTLVDASPRSAGKWL
                     LTGSQEAPLMRGVSESLAGRAAVLQLWPLSVEESPKVSLVRGGFPEVLARPRSARTWF
                     SSYVQTYLERDVRSVAAIRDLGSFRRFLGLVATRHGQTVNRTDLAAPLGVSVPTVSAW
                     LDILEVTGQILLLPPFFENFGKRLVKSPKLYLADSGLACFLLGIESEKQLDASPFLGP
                     VFEGYVASEIVKSQLNAGKRRELYHFRDQKGLEVDFVVPTGEGTVALVEAKATRTVRP
                     ELARNLEALGTAAGDGRSTAFVVHRGGTAGVESTTLRPSVKALPLPDLLAALR"
     gene            6636..6869
                     /locus_tag="P5164_09065"
     CDS             6636..6869
                     /locus_tag="P5164_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRY44082.1"
                     /translation="MTEVNVHEAKTHLSELLHRVEAGEEVVIARAGKPVARLVPVQPE
                     SSDRPLGLDAGLVRIAPDFDAPLPEEVLGDFER"
     gene            6866..7267
                     /locus_tag="P5164_09070"
     CDS             6866..7267
                     /locus_tag="P5164_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRY44083.1"
                     /translation="MNVLVDTHVWIWSLADPDRLSKECRKLLSSSRNVVYLSAASAWE
                     LATKAALGRIELPEPVETYVPTRMARQGITALPVTHVHALRVSTLPPHHRDPFDRLLV
                     AQALVERFPILTADPAFDRYDVQVIPAARRL"
     gene            complement(7303..7641)
                     /locus_tag="P5164_09075"
     CDS             complement(7303..7641)
                     /locus_tag="P5164_09075"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004668271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRY44084.1"
                     /translation="MKRGEVWWVDFEPSVGGEVRKKRPAVIVSNDASNQFLNRVQVVP
                     VTSNVDRLYPSEAYVSVKGQRRKAMTDQLTTVSKTRLSSRLGQLGHSDLEQVERAILV
                     QLGLPFPDRS"
     gene            complement(7628..7858)
                     /locus_tag="P5164_09080"
     CDS             complement(7628..7858)
                     /locus_tag="P5164_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014030533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HRY44085.1"
                     /translation="MQKKLTITVDERVYAGLHLVIGRHKISRFIESLVRPHVVGTDLV
                     AAYRAMGADEAREAEAHEWAEATVSDVAHETR"
     gene            complement(7918..8799)
                     /locus_tag="P5164_09085"
     CDS             complement(7918..8799)
                     /locus_tag="P5164_09085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY44086.1"
                     /translation="MPDSGLSVVIPTRDRCVVLEKCLGALEAQNEAAGSFEVVVVVDG
                     AGDGTNEMLARVSPRRFRLEVVSGPPRGPAAARNEGVRRARGGRVLLLGDDTFPAPGT
                     LGVHARFGAEEVGVQGRIEWDEAVGVTRVMQFLAPEGPQFYFRGLEDGAPMEYWRASA
                     SNLSVPRRWLLEEPFDEGFPDAAFEDTEAARRWDRRGWPVVWAEPAVCRHHHRYESIE
                     PFLARQRRAGRAARYAVTKDPSLAWRAFVQPVVAGAVSAARSAVARLSGQLEPERTWD
                     LACRRAFLAGFLGAGRD"
     gene            complement(8818..9228)
                     /locus_tag="P5164_09090"
     CDS             complement(8818..9228)
                     /locus_tag="P5164_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44087.1"
                     /translation="MEAVLGAAFLVRTKVFEELGGFDPRYFHFAEEDDLFRRIQDRGE
                     KVVFVPEPRVVHVGSGASEEIPIETLDRWKADGFLRYAAKHHGSDGRRRTRRALLLGA
                     RLRWLAASLPGALGSRERVRRFAALVTYLRTVGH"
BASE COUNT         1487 a         3375 c         3226 g         1383 t
ORIGIN      
        1 ggccgagttc cgggcgcggg ccggccccgc gttcgattga gaaaaccagg agaaggcatg
       61 ccgcgtcggc acccacgagg cgaggaatct cgccgtgccg tcggtcgacc cgtcgtcgcc
      121 gacgatcacc tcgaactcga ctccgggctc ctggcgctcc caccccgtga gcgcccgccg
      181 aaggaggtcg aggcggctga aggtcgggat gacgaccgag aacatcggcc ggggttcgcc
      241 cccgctcacg gcctcgagtc cccgcttccg ccgagcccct ctctccaccg ttcctccggc
      301 cgggtccggg cgatccagct ccgtgcggcg tcgaggccgc gccgggcgag ctcctcccgg
      361 gccgtcgccg aagcgagcgg tacgagggct gccgcgagcg ccgcgccgtc cgcccgctcc
      421 ttcacgaggg cgaccgcgtc gcgtgcgtcg agcgcgcggg cggtcggctc ggagacgagc
      481 gccgggactc cgcgcgcgag cgcctcgagg aggagctgcg cgcggaacgg acctgtgccg
      541 ggaaggcaga agaggtcggc ggcggagagg aggtcgtcga gtccctccgt cgcgacgcgg
      601 agcgaggcga gccggcggct cgcgaccagg tcggagacga cgggtcggag cggtccgttg
      661 ccaccgacga ggaacgtcca cccgccctga ccttcgagcg tcgccgcagc ctggaggagc
      721 tcgaagaccc cggaatcctc gtcgagcgtg tcggcgagga cgacggccac gttcccttcg
      781 gggactgcga gccgcgcccg ggcggcgatc cgcccctccg acggcaccgg ggccgcggcg
      841 cccgccggcg gcaggcacgg caggccgatc ccgtcggcgc gcccgtcttc cgagaggccg
      901 aggacacgga gctccgggtg tcgcagacga agggagcgcg cgaggaagag cgggtcgggg
      961 cgccagcgaa ggtcgacgac ggcttgcgca tgtcgccggc gcgcgacgag cccgagcgcc
     1021 gaaaggcgca acggcggcgg aaggacaatc ccggccgagt aggccgccac tccgtcggcc
     1081 ggctcctcct cgtccgcatc gaaggccacg aaaggcacct cggcaccgag cgcctccgca
     1141 agccggcgcg cgaaggccgt tccgccccac ccgctccggc tgccgacgac ggcgacgatc
     1201 ccgtcgttgc cggtcgccgc cgccggtgtg tctgcacttc cgaggaggcc atcgagcggc
     1261 gtcagctgcc tctcgagagg gagccccgag cgccaggctc cccgccgaac ccaaagcccg
     1321 ccgaccgaga cggtgtccgg cgggaccgcg atcgtcaccc gcccgacggc gccggagccc
     1381 gggaggctcc gccccacgac gcgacgcccc tcggttgagc cctcggcatc agggaggcgg
     1441 agcgacaggc tctgacggtc gagcaatgcc gcgcgggcga ggacgactcg ggcgctgcgg
     1501 ccctcgccct ccgcggtgaa gacgaggtcc tcgacggcgg cggcccagag gtgctcctcg
     1561 aggaacgtca gagagacggc tgccggggcg tctggcgcaa agaagagcca gcgcccccga
     1621 ggctccggtc cgggctccgc cccctcgccg ctcgcgggcg gcacgacgaa gacttccacg
     1681 tccggaaggc tctggtgtcc cagccattcg cgcaccgcgc cctcgctctc ccgcgtcacg
     1741 aggaccgtca cggcgggcga agggacggga gggcgagcca cgaggcgggt ttcgaagacc
     1801 cactcggccg gggggagagt cggcccctgc cgcagcttca gcgtccgcca ggcgcgtcgc
     1861 accccctgct tcccggccgc gcgcaccgct cgccaggcgc ctctcgccac cttccggagg
     1921 agcgacggct ggcccgcggc ggcgcgtccg gccgcctcct cgagccgccg gagccgcgcc
     1981 tcgaggtgcg tgatccgctc ctccagaatc agcggggccg gagcggagcc gggcgcgccc
     2041 gactccagga ggcgcaggag cttctccatc ccctggtgca cctcgagcag ctcccgccca
     2101 aggtcggaga tctcctcctg ggcccggcgc agccggacgt ccagatcgcg gctcaagatc
     2161 cctccgggcg cggaacctcc gtcccgcgcc tctcgacggc cagcgtcacg tcgtggggcc
     2221 ggaagagccc gaggacgcgg ctcgtcggaa cggaacggag gatcacgtct ccgtcgatcc
     2281 agtcgagcac ggtgtggttc tcctgcgtcc cgctcgccac ggcgggggag aggctgtagt
     2341 cggtctccgc gagctcgggc cacggaaacg cgaaccttac cgtgagcctc tctcccgccc
     2401 gagcgggggg gagcggcgtg ccgcgctcgg tcgtgttcat cgagatcagc tccctcccga
     2461 acgggtcgcg gatctggaaa ccgaggatcg gctccgcgac gtcggcgtga aaggcggcgt
     2521 cgaggacgac ctcgtatcgc tctccgggga cgagcatcgt ggtcgcgccc ccggtctcgt
     2581 cgacgactcc ccagcgctcg agccgggccc ggccgtcgcc gtagcgcagg acgccgctcc
     2641 ggtcgacctc ggaggagatc cggagctccg agtcgcggtt ctcgtagacc cacgcgaggt
     2701 agcgctggac gatctccctc gggctcgaga cgaggcgcac ccgcccctcc tcgatccaca
     2761 tcgcctcgtc gcagagcgac gagagggcgt tcatgtcgtg ggtcacgagg aggatcgtcg
     2821 tcccgcgggc ccggaggtcg gcgatcttct tcatgcagcg gtgctggaag acggcgtcgc
     2881 ctaccgcgag cgcctcgtcg accacgagca cctccgggtc gacgtgcgcc gccaccgaga
     2941 acgccaggcg gacgaacatc ccgctcgagt aggtcttcac cggctggtcc atgaagtcgg
     3001 ccagctggct gaacgcgacg atctcggggt agacccgctc gatctgttcg cggggcatcc
     3061 ccaggagcga ggcgttcagg aagacgttct cgcggcccgt gtactccgcg ttgaacccgg
     3121 cacccagctc gaggagcgcc gcgagccggc cccgcctccg gacgagcccc tccgtcggcg
     3181 cgaggaggtc gcagaccacc ttcaggagcg tgctcttgcc gctcccgttc gggccgatga
     3241 tcccgaacga ccgcccctcg gccacgtcga acgtcacgtc gcgcagcgcc cactgctcgc
     3301 gatagaagcg cttcctctct cccgccacga tctgcttcag ccggtcgaag gccgacgggt
     3361 agagccggta gcgcagcccg aggccccgca cctcgatggc ggccttcaca gcacctccac
     3421 gaacgccttg cgggtccggt ggaaccacca gtagccggcc gcgaacgaga cgagcgccat
     3481 ggcggcgagc gtcccgagcg aagcgaggtc gggtcctcgc ccgaagagga tcgcctcccg
     3541 ccagagccgg acgacgaccg cgagggggtt cacctcctgc acccagcgga accgggcggg
     3601 gaaggcggat tcggggtagg tgatcggcgt caggaagaag agcacctgca gggcgatgcc
     3661 gacgacggtg ccgaggtcgc gcacgaagac ggcgagcgac gacaggaaga aggtgagccc
     3721 cgccgtgagg atcaggtgca gggcgagcac cgggagcgcc cggagaaggc ccgtgagcgg
     3781 cgccccgagg gcgacgggag cgaggacgac gaggatcgac gtcgtcacgg cgagctggag
     3841 cagccccgag atgaggacgc tcgccggcag gacctcgagc gggaagacga ccttcgagac
     3901 gagccccccg tggctgacga tgaggctcga cgaccggatc agggccgtgg agaggaagct
     3961 gtaggggatg aagctcgcga agaggacgag gccgtaggag aggaagctct ccgagacgct
     4021 tccgccccat cgcatccgga agacgaacga gaagacgaac gcgtagacga agaggaggac
     4081 gagaggcgtc gcgaacgccc agaacgcccc gaggagcgac ccccggaacg gcccgagaaa
     4141 ctcccgcacc gcgagctgcc agaggaggta gcggtgctcg cggagcgtcg ccccgaaccg
     4201 ccggagcgac gggatttcca cgcgccccgg ccggatctcg acgacgtcgg tcatgcgcgc
     4261 gccattctcg cccagccgct cgtctcgcgc ctccctcgcc ccgtagactg tagagtgggg
     4321 gtcaggcccc gttttctggg tgattccaaa agtttcactt tagaatgctc tccatcttct
     4381 ggggtgcgtt cgtcgcggct taagatcggt tcggcagacc catgaatcgt ccgctcgaca
     4441 cgtcggaaga agcctgggcg atggtgcgca gcatccactc gcgtctcgga cccgagggac
     4501 gcgtcgaggc ggcgttcgcg gcttccgaga tgatccgaga agccgtccgg gcggggatcc
     4561 tcctgcgcca cccgggttgg gacgagcgcc agcttcgggg ggccgtactc gagcggttct
     4621 atggctctgc ccttgcggct cgggttctgg gagctgccgg gtcgaagctg tgacgtcgcc
     4681 gaaagggctc ctcgtccggc tcgcgggcgc cctcagcgcc tcagggatgc cgtggatgat
     4741 cacgggctcg acggcgtcgg ccttctacgg agagccgcgt gcgacacagg acgtcgacgt
     4801 cgtcgtcgac cccacgccgg accaggtctt gcgatttctc gacgagctcc gcgacggctt
     4861 ctacgtcagc cgggacgaag ccggcgatgc ccttcgtata cgggggatgt tcaacgtcat
     4921 cgatctcgaa tcgggttgga aggccggcat catcgtttgc cccgatcggc ccttcgagcg
     4981 gaacgagatc gctcggcgtc gacctgctcg cttgtttggc gtcgacgtct tcgtcatctc
     5041 gcccgaggga tccgtggtct cgaagctccg ttgggcggcc tcgtcgggct cagaacgcca
     5101 gctgcgagac gcggcctccg ttcttgccgg ctgccaaggt gacatcgatc tcgattacat
     5161 ccgtagggaa gccgaagtcg cgggggtctc ccacctgctg gcgaagatcc tcccgcccgg
     5221 gtaggtgccc tcgtcgcagc tcggcggtcc gggctcgacc tcggcgtgat gacgactctc
     5281 gtgtagagtg ggggtcaggc ccccttttct gggtgattcc taaagttcta ctttagaatt
     5341 ctccccatgc gttacgtctc tcgcaggatc gagcccgtgc ttcgaagagc cgcgcgggac
     5401 ttcccggtcg tcatcctcac gggtccgagg cgctccggaa agaccaccgt cctgcgccgg
     5461 gtgttcccgc gggcgagcta cgtcctcctg gaggatcccg acgttctgct ccgcgtcaag
     5521 ggcgatcctc gcggctttct cgaggaacgg aagctcccgg tcatcctcga cgagatccag
     5581 aatgctcccg agctcctggg ctacgtccgg acgctggtcg acgcgtcgcc ccgcagcgca
     5641 ggcaagtggc tcctgacggg ctcgcaggag gcgcccctga tgcgcggagt gagcgagtcg
     5701 ctggcaggga gggctgccgt ccttcagctc tggccgctct ccgtcgagga gtcgccgaag
     5761 gtgtccctcg tccggggtgg gttccctgag gtcctcgccc gcccgcgttc cgcgcgaact
     5821 tggttcagct cttacgtcca gacctacctg gagcgcgacg tccggtccgt cgccgcaatt
     5881 cgagacctcg ggtccttccg gaggttcctc ggtctcgtcg ccacgaggca cgggcagacc
     5941 gtcaaccgga ccgacctcgc ggctccgctc ggtgtttcgg tcccgacggt ctccgcctgg
     6001 ctcgacatcc tcgaggtcac cgggcagatc ctcctccttc ctcccttttt cgagaacttc
     6061 ggcaagcgcc tcgtgaagag cccgaagctg tacctggccg actcgggcct ggcgtgcttc
     6121 ctcctcggca tcgagagcga gaagcagctc gacgcatcac cgttcctggg ccccgtcttc
     6181 gaggggtacg tggcctcgga gatcgtcaag agccagctca atgcggggaa gcgacgggag
     6241 ctctaccact tccgcgatca gaaaggtctc gaggtcgact tcgtagtccc gacaggggaa
     6301 ggaaccgtcg cgctcgtcga agcgaaggcc acgcgcaccg tccgtccgga gctcgcacgg
     6361 aacctggaag cactggggac ggctgcaggc gacgggcgct ccacggcgtt cgtcgttcac
     6421 cggggcggga ccgcgggtgt cgagtcgacc acccttcggc ccagcgtgaa ggctcttccg
     6481 cttccggacc tcctcgcagc cctgcggtga gcggggcccg tcgggacgtg aatcagggcc
     6541 aggccccggg tggttccgcg agggaggctc ctccccgggc gacgccagca ctcctcgggt
     6601 ttgacttagt ccatagatag acttagtctt ttaggatgac cgaggtcaac gtccacgagg
     6661 caaagacgca cctgtccgag ctccttcacc gtgtcgaagc cggcgaagag gtcgtcatcg
     6721 ctcgcgcggg aaagccggtc gcgcgcctcg tgcccgttca gcccgagtcg tccgacaggc
     6781 ccctcggcct ggatgccggt ctcgtccgta tcgctcccga tttcgacgct ccgctgccgg
     6841 aggaagtgct cggcgatttc gagcgatgaa cgtcctcgtc gacacccacg tctggatctg
     6901 gagcctcgcg gatcccgacc ggctctcgaa ggagtgccgg aagctcctct cctccagccg
     6961 gaacgtcgtc tacctttcgg cggcgagcgc ctgggagctc gcgaccaagg ccgcgctcgg
     7021 gaggatcgag ctacccgagc cggtggagac ctacgtcccc actcgcatgg cacggcaggg
     7081 catcacggcg cttcccgtca cccacgtcca cgcgctccgg gtctcgacgc ttcccccgca
     7141 ccaccgagac ccgttcgacc gcctcctcgt cgcccaggcg ctcgtcgagc gcttcccaat
     7201 cctcacagcc gaccccgcct tcgaccgcta cgacgtccag gtcatccccg cggctcgtag
     7261 actgtagaga gtagaccaga ccccattcct tccggggagg agtcaagatc tatcggggaa
     7321 cgggagaccg agctggacga ggatggcccg ctcgacctgc tcgaggtcgg agtggccgag
     7381 ctggccgagc cgcgaggaga gcctcgtctt ggaaacggtc gtcagctggt cggtcatggc
     7441 cttccgacgc tgacctttga cggacacgta ggcttcactc gggtagagcc ggtcgacgtt
     7501 cgaggtcaca gggacgacct ggacgcgatt gaggaactgg ttcgaagcgt cgttgctgac
     7561 gatgaccgcg ggtcgcttct tccgtacctc tcctccgacg gatggctcga agtcgaccca
     7621 ccagacttca ccgcgtttca tgtgcgacgt ccgaaacggt agcctccgcc cattcgtgtg
     7681 cttcggcctc ccgtgcttcg tcggcgccca tggcgcggta ggcggcgacg aggtcagtcc
     7741 ccacgacgtg cggtcggacg agcgattcga tgaaccgact gatcttgtgg cgcccgatga
     7801 ccaggtggag cccggcgtag acgcgttcgt cgacggtgat cgtgagcttc ttctgcatac
     7861 gtatacgata cgtattcgcg gggccgacgt caaccaacgg aatcccaagt gtgcccgtca
     7921 gtctcgcccc gcgccgagga acccggccag gaacgctcgc cggcaggcaa ggtcccacgt
     7981 cctctcgggc tccagctgcc ccgagagcct cgccacggcc gacctcgccg cgctgacggc
     8041 ccccgcgacg accggctgca cgaaggccct ccaggcgaga ctggggtctt tcgtcacggc
     8101 atatcgagcc gcccgccccg cgcgacgctg gcgcgcgaga aacggctcga tcgactcgta
     8161 cctgtggtgg tgccggcaga cggccggctc cgcccagacg acgggccagc cgcgccggtc
     8221 ccaccgtcgc gcggcttcgg tgtcctcgaa cgcggcgtcc gggaagcctt cgtcgaacgg
     8281 ctcctcgagg agccaccgtc gaggcacgga gaggttcgag gcggaggctc gccagtactc
     8341 catcggcgct ccgtcctcga gccccctgaa gtagaactgc gggccctccg gcgcgaggaa
     8401 ctgcatcacg cgcgtcacac cgaccgcttc gtcccactcg atgcggccct gcacgccgac
     8461 ctcctccgcg ccgaagcggg cgtggacgcc gagagtcccg ggggcgggaa aggtgtcgtc
     8521 gcccagcagc agaacgcgtc cgccccgcgc ccgtcgcaca ccctcgttcc gagccgccgc
     8581 gggcccccgc ggtggcccgg agaccacctc gagccggaag cgcctcggcg agacccgcgc
     8641 gagcatctcg ttcgttccgt caccggctcc gtcgaccacg acgacgacct cgaagctccc
     8701 ggcggcttcg ttctgcgcct cgagcgcacc caggcacttc tccagaacga cgcatctgtc
     8761 gcgcgttggg atgacgacgg agagtccgga atccggcaac ggcaggtcct ccgcccgtca
     8821 atgcccgacg gtacgcaagt acgtcacgag ggcggcgaaa cgacggaccc gctctcgcga
     8881 accgagcgcc ccggggagcg acgccgcgag ccaccggagc cgtgccccca ggaggagcgc
     8941 gcgccgcgtc cgtcgacggc cgtccgaccc gtggtgcttt gccgcgtagc gcaggaaccc
     9001 gtccgccttc cagcggtcaa gcgtctcgat cggaatctcc tcgctggcgc ccgagcccac
     9061 gtggacgaca cgaggctccg gcacgaagac gaccttctcg ccccggtcct ggattcgccg
     9121 gaagaggtcg tcctcctccg cgaagtgaaa gtaccgtgga tcgaatccgc ccagctcttc
     9181 gaacaccttc gtccgcacga ggaaggcggc ccccagaacc gcctccaccg cacacccccg
     9241 gtgaggctgc cgaacggtgc tgttcccctc cagggcagct aagcacggca gaaggcgccc
     9301 gacgggaaca ttctccatca ggagccgcca cgggctcggg agccggtgaa ccgtcggaga
     9361 gggcaccccg gcgacgtcta cgagctgcgg accggcgacg gaggccttcg gatcggcatc
     9421 cagggcggca accagcactt ccactgttcc cgcggtaagg aggacgtcgt t
//
LOCUS       DAOTEZ010000144         9451 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5154_length_9451_cov_6.282354, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000144 DAOTEZ010000000
VERSION     DAOTEZ010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9451
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5154_length_9451_cov_6.282354"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="P5164_09095"
     CDS             complement(<1..869)
                     /locus_tag="P5164_09095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY44088.1"
                     /translation="MSLIEPSNRTADLSALKIRRDERPDRGRRVLPAVATAIVVAIAA
                     FLILGGRGVTLRAPEVSVTRVALVTPTQASTVLTASGYIVARSKAEISPKSVGRVAWL
                     NLEEGQKVRKGELVARLESQELEAQRKQYAAAREQALAELVNARTERQRAAQLLKEAV
                     GSQQAFDGADARVKALEAQVSSIEAQVRYVEELIRNAEIYAPIDGVVTVKKAFVGETV
                     SPQGFGGSGSAGATFAVIVDLGSLEMEADINEQNLGRLSIGQPAEVALDAYPDKPYKA
                     RLRQIVPTADRQKG"
     gene            complement(955..2403)
                     /locus_tag="P5164_09100"
     CDS             complement(955..2403)
                     /locus_tag="P5164_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HRY44089.1"
                     /translation="MNGDFSPDTLRAWGGAAVERLARYFETIESRPVLARTTPGELVA
                     QFPPYAPEDPEPWEAIERDLDRLVEPNLTHWQHPGFMAYFATNGSVPGIVGETLAAGY
                     NQVGILWRTSPASNEMEQAMVRWLANFVGLPADFDGQLADTASAASLIALAAAREQAF
                     PDVRKTGLSGQPPAVVYASEMAHSSIDKACVVLGLGISGLRKIPTDAEHRMQVDALEA
                     AIAADRAAGRKPIAVVATAGTTAVASIDPLPEIAALCRKEGLWLHVDAAYAGAAASLP
                     EKSPLFAGWEEADSIVWNPHKWLFLPLECTGLFFRRMERVRRAFSVVPTYLETPEGSA
                     GVREYMDYGIQLGRRFRALKAWILMRTFGSKGLRERLRSHIDLALQLEERLRREPDVE
                     ILAPVLFSVVVFRFAPAGLSEPERDALNQRVLEAVNRDGRSFLSHGVVKGKYALHAAI
                     GSLRTEERHLDAFLDSLRSGRALAETVKSSTP"
     gene            complement(2433..>2766)
                     /locus_tag="P5164_09105"
     CDS             complement(2433..>2766)
                     /locus_tag="P5164_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44090.1"
                     /translation="LADRFLMLEPLLSFLPPADQRRLAGERGFAALTWGKRTAWFLLV
                     YPGLTAPAHAARLLIGTGGLGSFLLLALALGLGVEQVLRLRTLARGEPAPSVLGRLVA
                     PFAAPLLR"
     assembly_gap    2767..2866
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2867..3366)
                     /locus_tag="P5164_09110"
     CDS             complement(<2867..3366)
                     /locus_tag="P5164_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44091.1"
                     /translation="MPRPFGPDRIELASSGHLRLTSPRSKEWVARRPAGPGNVLHPGT
                     AIRWEEELWEVVDAGDALGGGAWYELARWDDRHAIRLVLPYDETSEADGAEARRDVAR
                     RGGARLVALLLSPVVGLLPGHVQGRLEVELGVRGTTLTLASIPAPLAVGVGALLAMLA
                     SLVGAA"
     gene            3416..5218
                     /locus_tag="P5164_09115"
     CDS             3416..5218
                     /locus_tag="P5164_09115"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRY44092.1"
                     /translation="MPEGRLFRSLSKLHVRDLGRPVSGRSLRTQFDRAVAVVLRLLAL
                     LAVAALLLEHGFDLSPETKPAVHRLLEVVLLTFAGLGLIRLATRDDRRAFLREHRLDA
                     VVWGVVLLGFAGRRWGVSLPSVFFGLDPDGFAGAWLVATQGLIVVSILLGALRDSRRL
                     VGRRVQPQLVILGSFVLLIAVGTGFLLLPRATHAPGIGFVDALFTAASAASVTGLTVV
                     DTATRFTPLGQAFLLALIQAGGLGIMTLTTFFAFAFSGGTLRQYASLQSLLGEQSLGN
                     IRSTLAQIALVTLGFEAAGAAVLYRYLPESAVPEAGRLFAAVFHSVSAFCNAGFALHG
                     RNLAAPGLAENVVVQATVIVLVVAGGLGFPVLAALGAAALRRRRRLGLHVQLVLAVSG
                     LLLLAGTAGLYLLEPGGGALGASGGDRLLAAFFHSVSARTAGFNTVEMAQLGPATLFV
                     LMILMWVGGSPASTAGGIKTTTAAVALLTVLSIAGGKERVELFRRRLGDASIQRAFAT
                     VVVSVLLLSLSVLALLALEKQPPLDLAFEAVSALSTVGLSTGITASLGVPAKLLLSAL
                     MLAGRVGLLGCVLAATPLRRDARHEYAREDVLIT"
     gene            5224..5928
                     /locus_tag="P5164_09120"
     CDS             5224..5928
                     /locus_tag="P5164_09120"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRY44093.1"
                     /translation="MPRQFAVIGLGNFGSQLAAELTRRGAEVLAIDEREEAVDDLRDH
                     VTHTVVLDATDDQALAEQRVAEMDAVVVSFGSDFEAALLTVSVLKQIGVKRIIVRATT
                     VRHERILEALGVDEVVLPVNETVTRLAGSLMVEGLYDSFALSSDYAVGEVAIPEELLG
                     KKAGEVDFAADYGVSLVTIRRIVPETRILGLGTRMVERILGIPSPATPLERGDTLIVF
                     GPKKALEKLAKKGEPG"
     gene            complement(5947..6297)
                     /gene="speD"
                     /locus_tag="P5164_09125"
     CDS             complement(5947..6297)
                     /gene="speD"
                     /locus_tag="P5164_09125"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HRY44094.1"
                     /translation="MSPLGTHLLLDLAEAPFATLDDPAVVESALVETVAAMGAHVLGV
                     HLHRLAPQGISGVVVITESHLTIHTWPERGEAAVDLFTCGDPERARAAVAALVERLGA
                     GTSTLREVSRGAAR"
     gene            complement(6302..7426)
                     /locus_tag="P5164_09130"
     CDS             complement(6302..7426)
                     /locus_tag="P5164_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44095.1"
                     /translation="MSGDARSPWLFSRRADLLLFGGPALVSLLLLAGGARLGLLDGDL
                     PVPLWLLLVVGVDVAHVWSTGWRVYADPEELRRRPALYLGLPAAAYAAGVVLFTAGDL
                     VFWRVLAYLATFHFVRQQYGWVALYRRKSGENAEEGAAWERRLDAAAVYGATLAPLVF
                     WHARLPRRFHWLIAGDYAAGLPAWAGPAALATFGVIAVLWAAKEARRFVTGLPVSWGK
                     VLVVVTTALVWHLGIVVFDSDYAFAVTNVLVHGVPYFGLVLLSARSSAAARAAAGRKP
                     VLADFGARHAALFLAPLLLLAFLEEWGWDRLVWHENGALFPGGAIDPGPLLLSLVVPL
                     LALPQATHYLLDAWLWKVKPENRAAARAVGLAAPADADAG"
     gene            7534..8820
                     /locus_tag="P5164_09135"
     CDS             7534..8820
                     /locus_tag="P5164_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRY44096.1"
                     /translation="MSNHELSIVFFLALAAILLACRLVGYLARYVGQPQVVGEMIAGV
                     LLGPSLLGWIAPQAQAALFPKAAMPVLYVVAQIGLVLYMFLVGLDFDVELLRKRARSA
                     VTVSWAGILTPFALGALIAWAIHGDTALFAERVSVGEAALFMGAAMSITAFPMLARII
                     YERGLSGTSMGTLALAAGSADDAAAWCILAVVLASFSGQAAIAVWAIGGGLAFGLFAF
                     FVLRPLLARAGAWVERKQAYDSFALPGALMLLAAAAWYTDRVGIYAVFGAFVLGAAMP
                     RGEIAKRIEAQVQPLTVNLLLPLFFVYSGLNTRIGLVNSWALWGLALLVLLAATLGKG
                     GACYAAARLGGESHREAVGIGALMNARGLMELIILNIGLERGVITPTLFSIMVLMAIF
                     TTLTALPVFNLAFGKRVVGEEAVAAPAMAPGPLLDD"
     gene            complement(8835..>9451)
                     /locus_tag="P5164_09140"
     CDS             complement(8835..>9451)
                     /locus_tag="P5164_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="twin-arginine translocation pathway signal"
                     /protein_id="HRY44097.1"
                     /translation="ARHVVYALPRYTAAKVLAPWRESPPSFLRAFEYAPWLVANLTLS
                     GRPADRGFPLCWDNVLYDSRGLGYVVATHQGGRSHGPTVLTYYLVFAGEEPRKAREKL
                     LAATWKELAEAVLADLSRAHPDLPALVTNVDVYLWGHAMARPHPGFVWHPDREALERP
                     VGRVRFAHADASGLPLFEEAQDAGVRAAEAILSEEGESFRSLLG"
BASE COUNT         1246 a         3317 c         3467 g         1321 t
ORIGIN      
        1 cccttctgcc ggtcggccgt cgggacgatc tggcggaggc gcgccttgta gggcttgtcg
       61 ggatacgcgt cgagcgcgac ctccgccggc tgcccgatgg aaagccgccc gaggttctgc
      121 tcgttgatgt cggcctccat ctcgagcgag ccgaggtcga cgatgacggc gaacgtcgcc
      181 ccggccgagc cggagccgcc gaagccctgc ggggagaccg tctcgccgac gaacgccttc
      241 ttcacggtga cgacgccgtc gatcggggcg tagatctccg cgttgcggat cagctcctcg
      301 acgtagcgga cctgcgcctc gatggaggag acctgcgcct cgagcgcctt cacgcgggcg
      361 tcggccccgt cgaacgcctg ctggctcccg accgcctctt tcaggagctg cgcggcacgc
      421 tgccgctcgg tgcgggcgtt cacgagctcg gcgagagcct gctcgcgggc ggcggcgtac
      481 tgcttccgct gcgcctcgag ctcctgtgac tcgaggcggg cgacgagctc gcccttcctc
      541 accttctgcc cctcctcgag gttcagccag gcgacgcgcc cgaccgactt cggggagatc
      601 tcggccttgc tccgggcgac gatgtagccc gacgcggtga ggacggtcga cgcctgcgtc
      661 ggggtgacga gggcgacgcg ggtgacggag acctcggggg cgcggagggt cacgccgcgg
      721 ccgccgagga tgaggaaagc agcgatcgcg acgacgatcg ccgtcgcgac ggcgggaagg
      781 acccggcggc cccggtcggg gcgctcgtcg cgccggatct tcagggcgga gaggtccgcg
      841 gtgcggttgc tcggttcaat gaggctcatc tcgctctccc ggagggactt gcacacgcgg
      901 gccggccgcg cggggcgaca cttcggggct tcaccctccg gctcttcgcg taatctacgg
      961 ggtcgagctc ttcacggttt cagccagggc ccggcccgaa cgcagggagt cgaggaaggc
     1021 atccaggtgc cgctcctccg tccgaaggct cccgatcgcg gcgtggagcg cgtactttcc
     1081 cttcacgacg ccgtgggaga ggaacgagcg cccgtcgcgg ttcaccgcct cgaggacccg
     1141 ctggttcagc gcgtcgcgct ccggctcgga gaggccggcc ggggcgaagc ggaagacgac
     1201 gaccgagaag aggaccgggg cgaggatctc cacgtcgggc tcgcggcgga gccgctcctc
     1261 gagctgcagg gcgaggtcga tgtggctccg gagccgctcg cggagcccct tcgacccgaa
     1321 cgtccgcatc aggatccagg ctttcagcgc ccggaatcgc cggccgagct ggatgccgta
     1381 gtccatgtac tcgcggacgc cggcgctccc ctccggcgtc tcgaggtagg tcgggacgac
     1441 cgagaacgcg cgccgcaccc gctccatcct ccggaagaag aggccggtgc actcgagcgg
     1501 gaggaagagc cacttgtgcg ggttccagac gatcgagtcg gcctcctccc acccggcgaa
     1561 gagggggctc ttctcgggaa ggctcgccgc ggcgcccgcg taggcggcgt cgacgtggag
     1621 ccagagcccc tccttgcggc agagggcggc gatctccggc agggggtcga tcgacgcgac
     1681 ggcggtcgtc ccggccgttg ccacgacggc gatcggcttc cgtcccgccg cccggtcggc
     1741 ggcgatcgcc gcctcgagcg catcgacctg catccggtgc tccgcgtccg tcgggatctt
     1801 ccggaggccg gagatcccga ggccgaggac gacgcacgct ttgtcgatcg aggagtgggc
     1861 catctccgag gcgtagacga cggcaggggg ctggcccgag agccccgtct tccgcacgtc
     1921 ggggaaggcc tgctcgcgcg cggccgcgag ggcgatgagc gaggcggccg aggcggtgtc
     1981 ggcgagctgc ccgtcgaaat cggcgggcag cccgacgaaa ttcgcgagcc agcggaccat
     2041 cgcctgctcc atctcgttcg aggcgggcga ggtgcgccag aggattccga cctggttgta
     2101 gcccgcggcg agcgtctcgc cgacgatccc cggcacggag ccgttcgtcg cgaagtaggc
     2161 catgaagccg gggtgctgcc agtgcgtcag gttcggctcg acgaggcggt cgaggtcgcg
     2221 ctcgatcgcc tcccacggct cggggtcttc cggggcgtac ggcgggaact gcgcgacgag
     2281 ctcgcccggc gtcgtccggg cgaggaccgg cctgctctcg atcgtctcga agtagcgggc
     2341 gagccgctcc acggcggcgc cgccccaggc gcggagggtg tcgggggaga agtctccgtt
     2401 catggtccgc cgagtctatt gcggcaggaa cttcagcgga ggagcggggc tgcgaacggg
     2461 gcgacgagcc ggccgaggac gctcggcgcg ggctcgcccc gggcgagcgt ccggagccgg
     2521 aggacctgct cgacgccgag gccgagcgcg agggcgagga ggaggaacga cccgaggccg
     2581 ccggtcccga tcaggaggcg cgcggcgtgg gccggagcgg tgaggccggg atagacgagg
     2641 aggaaccagg ccgtccgctt tccccaggtc aacgccgcga aacctctctc cccggcgagc
     2701 cggcgctggt cggccggcgg gaggaaggag aggagcggct cgagcatcag gaaccggtcg
     2761 gcgaggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntggg cggcgccgac
     2881 gagcgacgcc agcatcgcga ggagggcgcc gacgccgacg gcgagcggcg cgggaatcga
     2941 ggcgagggtg agggtcgtgc cgcggacccc cagctcgacc tccagccggc cctgcacgtg
     3001 ccccggcagg agcccgacga cgggggagag gaggagcgcc acgagccgcg ccccaccccg
     3061 ccgggcgacg tcgcgacggg cctccgcgcc gtcggcctcg ctcgtctcgt cgtacgggag
     3121 gacgaggcgg atcgcgtgcc ggtcgtccca gcgggcgagc tcgtaccagg cgccgccccc
     3181 gagcgcgtcg ccggcgtcga cgacctccca gagctcctcc tcccagcgga tggccgtccc
     3241 cgggtgcagg acgtttccgg gcccggcggg ccggcgcgcg acccactcct tcgaacgggg
     3301 agaggtgagg cgcaggtgtc cggacgaggc gagctcgatc cggtcgggtc cgaacgggcg
     3361 cggcacgacg gccgactata cgttccgcgc ggtgcggaac gtagactcga gaccgatgcc
     3421 cgaggggcgg ctcttccgat cgctctcgaa gctccacgtg agggacctcg ggcgtccggt
     3481 ctccgggcga agcctccgga cgcagttcga ccgggccgtc gcggtcgtcc tgcgtctcct
     3541 cgcgctcctc gccgtcgcgg ccctcctcct cgagcacggc ttcgacctct cgccggagac
     3601 gaagcccgcc gtccaccggc tcctcgaggt cgtcctcctg acgtttgccg gcctcggcct
     3661 gatccggctc gccactcggg acgaccggcg cgcgttcctg cgcgagcatc gcctcgacgc
     3721 cgtcgtctgg ggcgtcgtcc tgctcgggtt cgcgggccgg cgatggggcg tctcgctgcc
     3781 ctctgtcttc ttcggcctcg acccggacgg gttcgccggg gcgtggctcg tcgcgacgca
     3841 ggggctgatc gtcgtctcga tcctcctcgg ggcgctgcgc gacagccgtc gcctcgtggg
     3901 ccgccgcgtc cagccccagc tcgtcatcct cgggagcttc gtcctcctca tcgccgtcgg
     3961 cacagggttc ctcctcctgc cgcgcgcgac gcacgcgccc ggcatcggct tcgtcgacgc
     4021 gctcttcacc gcggcgagcg cggcgtcggt gacggggctg accgtcgtcg acacggcgac
     4081 gaggttcacg cccctcggcc aggcgttcct cctcgcgctg atccaggccg ggggcctcgg
     4141 catcatgacg ctcacgacct tcttcgcttt cgccttctcc ggcgggaccc tccggcagta
     4201 cgcgagcctc cagtcgcttc tcggcgagca gagcctcggc aacatccgtt cgacgctcgc
     4261 ccagatcgcc ctcgtgacgc tcgggttcga ggccgccggg gccgcggtcc tctacaggta
     4321 cctccccgag agcgcggttc ccgaggccgg tcggctcttc gccgccgtct tccacagcgt
     4381 ctcggcgttc tgcaacgcgg ggttcgccct ccacgggcgg aacctcgcgg cgcccgggct
     4441 cgcggagaac gtcgtcgtcc aggcgaccgt catcgtcctc gtcgtcgcgg gaggcctcgg
     4501 cttcccggtc ctcgcggcgc tgggggcggc cgcgctgcgg cgtcgccgcc gcctcggcct
     4561 ccacgtccag ctcgtcctcg ccgtctcggg gctcctactc ctggccggta ccgcgggcct
     4621 ctacctgctc gagcccggcg gcggagcgct cggagcctcg ggcggcgacc gcctcctcgc
     4681 ggcgttcttc cattccgtct ccgcccggac ggcggggttc aacaccgtcg agatggccca
     4741 gctggggccg gccacgctct tcgtcctgat gatcctgatg tgggtcggcg gatcgccggc
     4801 ctcgaccgcc ggagggatca agacgacgac ggcggcggta gccctgctca cggtcctgtc
     4861 gatcgctggg ggcaaggagc gcgtcgagct gttccgtcgg cgactgggcg acgcgtcgat
     4921 ccagcgtgcg ttcgcgaccg tcgtcgtctc cgtgctgctc ctctcgctgt cggtcctcgc
     4981 cctgctcgcc ctcgagaagc agcccccgct cgacctggcc ttcgaggccg tatccgccct
     5041 ctcgaccgtg gggctttcga cggggatcac ggcgtccctc ggcgttccgg cgaagctcct
     5101 cctttcggcc ctcatgctgg cggggcgcgt cggccttctc ggctgcgtcc tcgccgccac
     5161 gccgctccgg cgcgacgcac gacacgagta cgcccgggaa gacgtcctga taacgtaacg
     5221 accatgccga gacagttcgc cgtcatcggg ctcgggaact tcggctccca gctcgccgcc
     5281 gagctcaccc gacggggggc cgaggtcctc gcgatcgacg agcgcgagga ggccgtcgac
     5341 gacctgcggg accacgtcac gcacaccgtc gtcctcgacg cgaccgacga ccaggcgctc
     5401 gccgagcagc gagtcgccga gatggatgcc gtcgtcgtca gcttcgggag cgacttcgag
     5461 gcggcgctcc tcacggtctc ggtcctcaag cagatcggtg tgaagaggat catcgtccgg
     5521 gccacgacgg tccggcacga gcggatcctg gaggcgctcg gcgtcgacga ggtcgtcctg
     5581 ccggtcaacg agacggtcac gcgcctcgcc gggagcctga tggtggaggg gctctacgac
     5641 tccttcgccc tctcgtcgga ctacgccgtc ggggaggtcg cgatcccgga ggagctcctc
     5701 ggaaagaagg cgggggaggt cgacttcgcg gccgactacg gcgtctcgct cgtcacgatc
     5761 cgtcggatcg tccccgagac gcggatcctc ggcctcggca cgcggatggt cgagcggatc
     5821 ctcggaatac catcgcccgc caccccgctc gagcgcggag acacgctgat cgtcttcggt
     5881 ccgaagaagg cgctcgagaa gctcgcgaag aagggcgagc cgggctgagc cggccgccgg
     5941 gagccgtcag cgggcggcgc cccgggagac ttctctgagg gtcgacgttc cggcgccgag
     6001 tctctcgacg agcgcggcga cggccgcgcg cgcgcgctcc gggtcgccgc aggtgaagag
     6061 gtcgacggcc gcctcgccgc gctccggcca ggtgtggatc gtcaggtgcg actcggtgat
     6121 gacgacgacg ccggagattc cctgcggcgc gaggcgatgg aggtggacgc cgaggacgtg
     6181 agcgcccatg gccgcgaccg tctcgacgag ggccgactcg acgacggcgg gatcgtcgag
     6241 cgtcgcgaac ggggcttcgg cgaggtcgag gaggaggtgc gtcccgagcg ggctcacgcg
     6301 ctcaccccgc gtccgcatcc gcgggtgccg cgaggccgac cgcccgggcc gccgcccgat
     6361 tctccggctt caccttccat agccacgcgt ccagaagata gtgcgtcgcc tgggggaggg
     6421 caaggagcgg cacgacgagc gagaggagga gcgggccggg gtcgatcgcg cctccgggga
     6481 agagggcgcc gttctcatgc cagacgaggc ggtcccagcc ccactcctcg aggaaggcga
     6541 ggaggaggag gggggccagg aagagggcgg cgtggcgcgc gccgaagtcc gcgaggaccg
     6601 gctttcggcc ggccgcggcc cgcgcggcgg ccgaggagcg agccgagagg aggacgaggc
     6661 cgaagtacgg gacgccgtgg acgaggacgt tcgtgacggc gaaggcgtag tcggagtcga
     6721 agacgacgat cccgaggtgc cagacgaggg ccgtcgtgac gacgacgagg acctttcccc
     6781 aggacacggg gagaccggtg acgaagcggc gggcctcctt cgcagcccag agcacggcga
     6841 tcacaccgaa cgtggcgagc gcggccgggc cggcccacgc ggggagcccg gccgcatagt
     6901 cccccgcgat cagccagtga aaccgcctgg gaagccgggc gtgccagaag acgagcggcg
     6961 cgagcgtcgc gccgtagacg gccgccgcgt cgaggcgacg ctcccacgcg gcgccctcct
     7021 cggcgttttc gcccgacttc ctccggtaga gcgcgaccca gccgtactgc tgccgcacga
     7081 agtggaacgt cgcgaggtag gcgaggacgc gccagaagac gaggtccccc gccgtgaaga
     7141 ggacgacgcc ggccgcgtac gccgccgccg gaaggccgag gtagagcgcc ggtcggcgcc
     7201 gcagctcttc ggggtcggcg tatacgcgcc agccggtcga ccagacgtgc gcgacgtcga
     7261 cgccgacgac gaggaggagc cagaggggga ccgggaggtc gccgtcgagg aggccgaggc
     7321 gcgcgccccc ggcaaggagg aggagcgaca cgagcgccgg cccgccgaag aggaggaggt
     7381 cggcgcggcg ggagaagagc cagggggacc gagcgtcgcc ggacaagggc ggcgacgagt
     7441 ctagcggctc ccctccccgc cggcgggaaa gcccctggcc cgggcgaggc gggcctccgc
     7501 tcggagctgc ggataggcta gagtgcgcgc cgcgtgtcca accacgagct ttcgatcgtc
     7561 ttcttcctcg cgctcgcggc gatcctcctc gcctgccgtc tcgtcggcta cctcgcccgc
     7621 tacgtcgggc agccgcaggt cgtcggcgag atgatcgcgg gggtcctcct cggcccgtcg
     7681 ctcctcggct ggatcgcccc gcaggcccag gccgcgctct tcccgaaggc cgcgatgccg
     7741 gtcctctacg tcgtcgcgca gatcggtctc gtcctctaca tgttcctcgt cgggctcgac
     7801 ttcgacgtgg agctcctccg gaagagggcc cggagcgccg tgaccgtgtc gtgggccggc
     7861 atcctgacgc cgttcgcgct cggggctctc atcgcgtggg cgatccacgg agacacggcg
     7921 ctcttcgccg agagggtctc cgtcggcgag gccgcgctct tcatgggcgc ggcgatgtcg
     7981 atcaccgcct tcccgatgct cgcgcggatc atctacgagc gcggcctctc ggggacctcg
     8041 atggggacgc tcgcgctcgc ggcgggctcg gccgacgacg cggccgcctg gtgcatcctc
     8101 gccgtcgtcc tcgcgagctt ctcgggccag gccgcgatcg ccgtctgggc gatcggcggc
     8161 ggcctcgcgt tcggcctctt cgcgttcttc gtcctcaggc cgctcctggc ccgcgcggga
     8221 gcgtgggtcg agaggaagca ggcgtacgac agcttcgcgc tccccggagc cctcatgctc
     8281 ctcgcggccg ctgcctggta caccgacagg gtcgggatct acgcggtctt cggcgccttc
     8341 gtcctcgggg ccgcgatgcc gcgcggggag atcgcgaagc ggatcgaggc gcaggtccag
     8401 cccctcaccg tgaaccttct cctgccgctc ttcttcgtct actcggggct caacacgcgg
     8461 atcggcctcg tgaactcctg ggcgctctgg ggcctcgccc tcctcgtcct cctcgcggcg
     8521 acgctcggaa agggaggcgc ctgctacgcg gcggcgcgcc tcggcggcga gtcgcatcgc
     8581 gaggcggtcg ggatcggggc cctcatgaac gcccgcggcc tgatggagct gatcatcctg
     8641 aacatcggcc tggagcgcgg cgtcatcacg ccgacgctct tctcgatcat ggtcctgatg
     8701 gcgatcttca cgacgttgac cgccctcccc gtcttcaacc tcgcgttcgg gaagagggtc
     8761 gtcggcgagg aggccgtcgc ggcccccgcg atggcccccg gacccctcct cgacgactga
     8821 tcgccgcggc cggcctaccc gaggagggag cggaagctct ctccctcttc ggagaggatc
     8881 gcctcggcgg cccggacccc cgcgtcctga gcctcctcga agagcgggag cccggaggcg
     8941 tcggcgtggg cgaagcggac gcggccgacg ggccgctcca gggcttcccg gtcggggtgc
     9001 cagacgaagc cggggtgcgg gcgcgccatc gcgtgccccc agaggtagac gtcgacgttc
     9061 gtgacgagcg cggggaggtc ggggtgcgca cgggagaggt cggcgagaac cgcctccgcg
     9121 agctccttcc aggtcgcggc gaggagcttc tcgcgcgcct tccgcggctc ctcgcccgcg
     9181 aagacgaggt agtaggtgag gacggtcgga ccgtggctgc gccccccctg gtgggtcgcg
     9241 acgacgtagc cgagcccccg ggagtcgtag aggacgttgt cccagcagag ggggaagcct
     9301 cggtcggccg ggcgtcccga gagggtgagg ttcgcgacga gccacggcgc gtactcgaac
     9361 gcccggagga acgagggagg gctctcgcgc cacggcgcga ggaccttcgc cgcggtgtag
     9421 cgggggagcg cgtagacgac gtgccgggcc g
//
LOCUS       DAOTEZ010000145         9430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5168_length_9430_cov_5.543253, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000145 DAOTEZ010000000
VERSION     DAOTEZ010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9430
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5168_length_9430_cov_5.543253"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="P5164_09145"
     CDS             complement(<1..1219)
                     /locus_tag="P5164_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY44098.1"
                     /translation="MPIRARIPSLSDWKAQVKCQAGCPVSTDAGRYVQLVAENRDEEA
                     FLVARAPNPFASVCGRVCAAPCEDACRRGTIDAPVSIRALKRFVTERYGVESVRPQTQ
                     DRLFGELVGEGNRYAGHLPVVPSPAPAPASGKTRIAVVGAGPAGLSAAHDLALLGYDV
                     TVFEAAGEPGGMMRFGIPEYRLPRTLIKAEIDRILALGVTLRLGTRLSPSFGLAELRS
                     GGFEAVFLSVGVSQGRELDAPGVELDGVVKAVDYLLNVNRGFRMDLGRRVVVVGGGFV
                     AFDAARTALRVFQEGELEALEEAAGAADARAKEAFDSARAALRGGAAEVTIVSLENFD
                     EMPVLRSTQGHEEFEEAQREGVAFLPRRGPKRFLGTSRLEAVELRKVLSVFDENGRFS
                     PRYDDSDVVTLPAD"
     gene            complement(1344..3632)
                     /locus_tag="P5164_09150"
     CDS             complement(1344..3632)
                     /locus_tag="P5164_09150"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRY44099.1"
                     /translation="MKLKSCLLVALLSARCLASEPAPATLSGSILAADGSGLPGARVS
                     VVASPGGSPPAAVVASGERGLYLVRGLRPGTYQLVVELPGFETGTRTDVSLSVGERRL
                     LDVTLRLAEVRERFTVVGPSPRDAVEAAEIRESPARDAGEALEKLPGVWKLRKGGIAN
                     DVVVRGSQSRDLNVLVDGERIYGACPNHMDPPSFHVDFAQVERVEVAKGPFDVRNQGS
                     LGGVVNIVTRRPEPGLHATANAAAGAWGYVNPSVTASWAGASFSALAGYSFRRSEAFA
                     GGDGRRFTERTNYRAEAVDAEAFRAGTAWARVGFVPAEGHTVVVSYTRQETSRTYYPY
                     LQMDAVYDDADRFGLTWEATPSAVVTSARLSGYYSQVDHFMTDEYRTSSATAARGWSM
                     GTDAFSRTLGGRLEATVRGVTIGAEAYRRYWDTTTYLAGRKYEPQASLPGATNDLVGL
                     FAERSFRLGDAVTLDAGLRWDHASASVDDAKASTDLAWAYHGTRATRRADSLPGGSLR
                     ARWRAVEGLEVSLGVGTAARVPEPQERWFSLKRMGTDWVGNPDLAPSRNTSLDLSASL
                     RSGRLYVSASAFVNDVSDYVVVSDAKKVNTVPGVMNPAARTYENVDARLAGGEATAAF
                     TLTDRLFFSSAVSYVRGTQDPRPELGILSKDLAEIPPVTGRASLRWDDGRYWGEVEGV
                     FAAAQSNVDTDVAETATPGWGVANLRAGLTLGSVGLTLGVENLFGRLYSEHLSYQRDP
                     FRSGVKVPEPGRNVFVNLSWRY"
     gene            complement(3716..4123)
                     /locus_tag="P5164_09155"
     CDS             complement(3716..4123)
                     /locus_tag="P5164_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44100.1"
                     /translation="MRQSRLRRSAGALVLVLAATLLLPVLALARVTAPPAPAHGCHCP
                     VKMACCEAGLCHGDMEPERSAAPSWSGCRDEAPHRAVVPPPSAAFDGALPVEETPAAS
                     EAGAPIALTTPEGGNAPRPDPATPPPRASIASR"
     gene            4312..6504
                     /locus_tag="P5164_09160"
     CDS             4312..6504
                     /locus_tag="P5164_09160"
                     /inference="COORDINATES: protein motif:HMM:NF033936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SO_0444 family Cu/Zn efflux transporter"
                     /protein_id="HRY44101.1"
                     /translation="MSLLVRVFEETYRIVLDSGFFILLGFLLAGLLHELVDTARIGRA
                     LGGRSLGAILKAAIVGGPLPLCSCGVLPMAVALRKKGASREATVSFLISTPETSEEAI
                     LLTWGLLGPVMALVRPVVGVVTAIVAGLVSLVAGGEDDRRSTEAGEAAGVEAAPCSTE
                     DGCATPEAPAARGGTLRERAARVFRYAFVTMVDELAFWMLLAFLATGVLAGVLPEDFF
                     LRFLPWPLLTMVLMALVGLPMYVCASASTPIAAAMIAKGLDPGSALVFMLTGPATNAS
                     TIAVVARMFGRRFVGVYLGSIVGVAIASGLLLNAVVGAGLVPAPGGPEAESGGAWAVV
                     RFLSAFAFLALMAFSFQRRGFRPGWNELKGHALAFRDLVREQAPRRPPRRALAGFGAA
                     VLLLLWGRSALLVVRPDETGLVRTLGKVTASDLAPGLHLVPPFPFGRAEAIPTARLRS
                     VEIGFRYRAAGSAAAPVTSRVFVPTQSTRVPEEAQFLTGDENVIEVTAVVHYRIADPA
                     AFRFGVDRAEPAFRDLARALLVEEVARTPIDAVYTAERGAVEKRVLEALRSAPVLAET
                     GLAPVDLRLLFVHAPDDVHGAFRDIASAAEDRTTARNKALVEAEGALGQARGEVARTL
                     ADADSERTRLVEHAKGDAAAFVPLAREVAAAPRTSRLRLKLEALERVLPGVPKLIRPD
                     PRRAPGFELWVTPASPADAASAPAPMKGLAGFVSPIPAPPPDVPAAER"
     gene            6515..7447
                     /gene="hflC"
                     /locus_tag="P5164_09165"
     CDS             6515..7447
                     /gene="hflC"
                     /locus_tag="P5164_09165"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HRY44102.1"
                     /translation="MEVPLEPRPSARRRRVATVAAGAALLLLVARVPYVVEATEYAVR
                     TRFGRPVAVVTEPGLHARVPLVDDVARLDARLLYFDPPAGEFLTNDKKNIVVSPVVLW
                     RIEKPLKFLQTLVTRESAEARLADLVASETGAALGAVPFTRLVSATDGEADLAGVVAG
                     IAERVRARAAADYGVKVVDVRLKRIAFPEQNLLSVFGRMRSERERIARRFRSEGEEAA
                     LKIRAETDREKTRLLSEAYRKAEEEKGAGEAEAARIYAKATNQDPDLYKFLRTLRAYE
                     TILDEKTTLVLPGDSDVLRLLTDGAAPSPKKGPN"
     gene            7444..8376
                     /gene="hflK"
                     /locus_tag="P5164_09170"
     CDS             7444..8376
                     /gene="hflK"
                     /locus_tag="P5164_09170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HRY44103.1"
                     /translation="MTRTARRALFAASGLAFGAWLLAGVSVLRPAQQGVVLRLGAVQP
                     LPLGPGIHWRPAAIDRVVKVEVTRTETVPIGFRTADGGRAMPLPAEEGQWLTGDTNIL
                     AVQLAVQYRVARPVEWVVHGRDTAEVVRRAAESVVTESMGVLPVDDALTGGRHTLLEE
                     VRRGTQELLDRWQSGVAVVSVALRSVDPPPPVLAAFQDVQDAKSDRERFVNEAESYAN
                     DTLPRARGEAEALVSAAASQRQRRVEEAHGDAARFDALRREASLAPGPLRARLYLETL
                     ERVLPRMRLYVVDEATRLRLVKPEPATPGAVPGG"
     gene            complement(8396..>9430)
                     /locus_tag="P5164_09175"
     CDS             complement(8396..>9430)
                     /locus_tag="P5164_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRY44104.1"
                     /translation="KEAFVLRTPVVFLTGASGEIGHTLIERLSEDGSRPIITLDLKPL
                     DPSIAPRVARQFTGSILEPQLLERILSEFEVDQVFHLAALLSTRSEFTPVTAHQVNVE
                     GTMAMLEFAQKQGESHGRPVVFMYPSSIAAYGMPGLDVKAKAGRVKEDDFTNPTTMYG
                     CNKLYCEQLGRYYARHYKQLAPETLSGKVDFRSIRFPGLISAFTVPSGGTSDFAPEMI
                     HAAAQGKPYACFVRPDARIPFMAMPDAVDALLQLAAAPREKLTRTTYNIGAFAPSAAE
                     IQAIVEQAFPGAEITTKVDAKRQGIVDSWPADVDDSAARKDWGLSPRYDLDRAFQEYL
                     IPNIRKAYQG"
BASE COUNT         1277 a         3318 c         3530 g         1305 t
ORIGIN      
        1 agtcggccgg aagcgtgacg acgtcggaat cgtcgtagcg gggggagaag cgtccgttct
       61 cgtcgaagac ggagaggacc ttccggagct cgacggcctc gaggcgggac gtcccgagga
      121 atcgcttcgg gccgcgccgg gggaggaagg cgacgccctc gcgctgcgcc tcctcgaact
      181 cctcgtgccc ctgcgtcgac cgcaggaccg gcatctcgtc gaagttctcg agcgagacga
      241 tcgtcacctc ggccgcgccg ccgcggagcg cggcccgggc cgagtcgaac gcctccttgg
      301 cgcgggcgtc ggcggctccc gccgcctcct cgagcgcctc gagctctccc tcctggaaga
      361 cgcggagcgc ggtgcgggcc gcgtcgaagg cgacgaagcc gcccccgacg acgacgacgc
      421 gccggccgag gtccatccgg aagccccggt tcacgttcag gaggtagtcg acggccttca
      481 cgacgccgtc gagctcgacg ccgggggcgt cgagctcgcg cccctgcgag acgccgacgg
      541 agaggaagac ggcctcgaag ccgccgctgc ggagctcggc gaggccgaac gacggcgaga
      601 gccgcgttcc gagccgcaaa gtcacgccga gggcgaggat ccggtcgatc tcggccttga
      661 tgagcgtccg cgggaggcgg tactcgggga tgccgaaccg catcattccg cccggctcgc
      721 ccgcggcctc gaagacggtg acgtcgtagc cgaggagggc gaggtcgtgc gcggccgaga
      781 ggcccgccgg tcccgcgccc acgaccgcga tgcgcgtctt ccccgacgcg ggcgccgggg
      841 cgggggaggg cacgacggga aggtggcccg cgtagcggtt cccctcgccg acgagctcgc
      901 cgaagagccg gtcctgcgtc tgggggcgga ccgattcgac gccgtagcgc tccgtcacga
      961 accgcttgag cgcccggatg gagacggggg cgtcgatcgt tccgcggcgg caggcgtcct
     1021 cgcagggcgc ggcgcagacc cggccgcaga ccgaggcgaa cgggttcggc gcgcgggcga
     1081 cgaggaaggc ctcctcgtca cggttctccg cgacgagctg gacgtaacgc ccggcatcgg
     1141 tcgacaccgg gcaacctgcc tggcacttga cctgcgcctt ccaatccgac aaggacggga
     1201 tgcgagcccg gatcggcatg tcgaatctcc ctgtgcgaga gaggatctgc cgcgcatcgc
     1261 ccggccggta tgacgcaggt catgacgagc aggtgaaagg ggaagccggg gccgccgatc
     1321 ctcggccccg gctctcggag gggtcagtaa cgccaggaaa ggttgacgaa gacgttccgg
     1381 ccgggctccg ggaccttcac gcccgaccgg aacgggtccc gctggtagga gaggtgctcg
     1441 gagtagaggc gaccgaagag gttctcgacg cccagggtca ggccgacgct cccgagggtg
     1501 agcccggcgc gcagattcgc gacgccccag ccgggggtcg ccgtctcggc gacgtccgtg
     1561 tcgacgttcg actgtgcggc ggcgaagacg ccctccacct cgccccagta gcggccgtcg
     1621 tcccagcgga gcgatgcccg accggtcacg gggggtatct ccgcgaggtc cttcgagagg
     1681 atgccgagct cgggccgcgg gtcctgtgtc ccgcggacgt acgacacggc gctcgagaag
     1741 aagaggcggt ccgtcagggt gaaggccgcg gtcgcctcgc cgccggcgag ccgggcgtcg
     1801 acgttctcat acgtgcgggc cgcggggttc atgacgccgg gaaccgtgtt caccttcttc
     1861 gcgtcggaga cgacgacgta gtcggaaacg tcgttgacga aagcgctcgc cgagacgtag
     1921 agacgcccgc tgcggagcga cgccgagagg tcgagagagg tgttccggga aggggcgagg
     1981 tccgggttcc cgacccagtc ggttcccatg cgtttcagcg agaaccacct ctcctgcggt
     2041 tccggaacgc gtgcggccgt cccgacgccg agggagacct cgagcccctc cacggcccgc
     2101 caccgggcgc ggaggctccc tccgggaagg gagtcggcgc ggcgcgtggc gcgcgtgccg
     2161 tgataggccc aggccaggtc cgtcgaggcc ttcgcgtcgt cgaccgacgc cgaggcgtgg
     2221 tcccagcgga gcccggcgtc gagcgtcacg gcgtcgccga gacggaacga cctttcggcg
     2281 aagaggccga cgaggtcgtt cgtcgctccg gggagcgagg cctgcggctc gtactttctg
     2341 cccgcgaggt acgtcgtggt gtcccagtac ctccggtagg cctcggcgcc gatcgtcacg
     2401 ccgcgaacgg tcgcctccag ccggccgccg agcgtcctcg agaaggcgtc ggttcccatc
     2461 gaccagccgc gcgccgcggt cgccgaggag gtccggtact cgtccgtcat gaagtggtcg
     2521 acctgcgagt agtagccgga gagccgggcc gacgtgacga cggcggaagg tgtcgcttcc
     2581 cacgtcaggc cgaagcggtc cgcgtcgtcg tacacggcgt ccatctgaag gtaggggtag
     2641 taggtgcggg aggtctcctg ccgcgtgtag gagacgacga ccgtgtggcc ctccgccggc
     2701 acgaacccga cgcgggccca cgcggtcccc gcccggaacg cctccgcgtc gacggcctcg
     2761 gcgcggtagt tcgtccgctc ggtgaagcgg cggccgtcgc cgcccgcgaa cgcttccgag
     2821 cgccggaagg agtacccggc gagcgccgag aacgacgcgc cggcccacga ggcggtgacc
     2881 gacgggttga cgtagcccca ggcccccgcg gcggcgttcg cggtggcgtg gaggcccggc
     2941 tcgggccggc gcgtcacgat gttcacgacg ccgccgaggc tcccctggtt ccggacgtcg
     3001 aacggtccct tcgccacctc gacccgctcg acctgggcga agtcgacgtg gaaggagggc
     3061 gggtccatgt ggttcgggca ggcgccgtag atccgctcgc cgtcgacgag gacgttcagg
     3121 tcgcggctct gggaaccgcg gacgacgaca tcgttcgcga tcccgccttt ccggagcttc
     3181 cagacgcccg ggagcttctc gagcgcctcg cccgcgtcgc gtgccgggct ctcgcggatc
     3241 tccgcggctt cgaccgcgtc gcggggtgac gggccgacga ccgtgaaccg ttcgcgcacc
     3301 tcggcgaggc ggagcgtcac gtcgagcagg cgccgttcgc cgacggagag cgagacgtcc
     3361 gtccgggttc ccgtctcgaa gcccggcagc tcgacgacga gctggtaggt cccgggacgg
     3421 agcccgcgga cgagatagag cccccgttcg ccggaggcga cgacggcggc cggcggggag
     3481 ccgccgggcg aggcgaccac ggagacacgg gccccgggga ggccgctccc gtccgcggcg
     3541 aggatcgagc ccgagagcgt cgccggagcg ggctccgacg cgagacagcg ggccgagagg
     3601 agggcgacga ggaggcagga cttcagcttc atgagtgctc catcgcggtt cgtctacgga
     3661 ctccgaacgg cggtgccgcc cggagcgccg aagggagggg ccgagccccg gaccgtcagc
     3721 gggaggcgat ggaggcccga ggaggcggag tcgccgggtc gggacggggc gcgttcccgc
     3781 cttcgggtgt cgtgagagcg atcggagctc cggcctcgga cgccgccggc gtctcctcga
     3841 ccgggagggc gccgtcgaac gcggccgacg ggggaggaac gaccgcccga tggggggcct
     3901 cgtcgcggca gcccgaccag gagggggcgg cggagcgttc gggctccatg tctccatggc
     3961 agagcccggc ttcgcagcag gccatcttca cggggcagtg acagccgtgc gcgggtgcgg
     4021 gaggagcggt gaccctcgcg agggcgagga caggcagcag cagcgtcgcc gcaaggacga
     4081 ggacgagcgc cccggccgag cgccgcaacc tgctctgtct catgacgggt cccttctagg
     4141 tccgatcggg cgctccgagt atgacgcgga tcatccgccg ggctctttcg cagggagcgg
     4201 cgattggaga ggcagtcgtc gacgccaccg ggccgcgtcg cttcgccggc gtacggggag
     4261 gggcgatact ccagccgtga aagcggaccg cgaccggcgc tccggcgtcc cgtgagcctc
     4321 ctcgtccggg tcttcgagga gacctaccgg atcgtcctcg acagcggctt cttcattctc
     4381 ctcgggttcc tcctcgccgg gctcctccac gagctcgtcg acacggcccg gatcgggcgc
     4441 gccctcggag ggaggagcct cggggcgatc ctgaaggcgg cgatcgtcgg cggtccgctg
     4501 ccgctctgct cctgcggggt gctgccgatg gcggttgcgc tccggaagaa gggggcgagc
     4561 cgcgaggcca ccgtctcgtt cctgatctcg acgccggaga cgagcgagga ggcgatcctc
     4621 ctgacgtggg ggctcctcgg ccccgtcatg gcgctcgtcc ggcccgtcgt cggcgtcgtg
     4681 accgcgatcg tggccgggct cgtctctctc gtcgcgggcg gggaggacga tcgacgttcc
     4741 acggaagcgg gtgaggcggc cggcgtcgag gcggccccct gctcgaccga agacggctgc
     4801 gcgacgcccg aagcccccgc cgcgcggggc gggaccttgc gggagcgggc cgcgcgggtc
     4861 ttccggtacg cgttcgtcac gatggtcgac gagctcgcgt tctggatgct cctggcgttc
     4921 ctcgcgacgg gagtgctggc gggcgtcctt ccggaagact tcttcttgcg gttcctcccc
     4981 tggccgctcc tgaccatggt cctgatggcc ctcgtcgggc tcccgatgta cgtctgcgcg
     5041 agcgcctcga cgcccatcgc cgccgcgatg atcgccaagg gcctcgaccc gggctcggcg
     5101 ctcgtcttca tgctgacggg tcccgcgacg aacgcctcga cgatcgcggt cgtcgcccga
     5161 atgttcggac ggcggttcgt cggggtctac ctcgggtcga tcgtcggcgt cgcgatcgcg
     5221 tcggggctcc tcctgaacgc cgtcgtcggc gcgggcctgg tcccggcgcc cggcggcccc
     5281 gaggcggaga gcgggggcgc ctgggccgtc gtcaggttcc tctcggcctt cgcgttcctc
     5341 gccctcatgg ccttcagctt ccagagaagg ggcttccggc cgggatggaa cgagctgaag
     5401 gggcacgcgc tcgcgttccg cgacctcgtt cgcgagcagg cgcccaggcg ccccccgcgg
     5461 cgggcgctcg ccggcttcgg cgcggcggtc cttctccttc tctggggccg gagcgcgctt
     5521 ctcgtcgtcc gccccgacga gacgggcctc gtccggacgc tcggaaaggt gactgcgtcc
     5581 gatctcgccc cgggtcttca cctcgtcccg ccgttcccgt tcgggagagc cgaggcgatc
     5641 ccgaccgcgc ggctgcgctc cgtcgagatc ggctttcgct acagggccgc cggctccgcg
     5701 gccgcgcccg tcacgtcgag ggtcttcgtc ccgacgcagt cgacgcgggt cccggaggag
     5761 gcgcagttcc tgacgggcga cgagaacgtc atcgaggtga cggccgtcgt ccactacagg
     5821 atcgccgacc cggccgcgtt ccgattcggc gtcgaccgcg ccgagccggc gttccgggac
     5881 ctcgcgcggg cgctcctcgt cgaggaggtg gctcggacgc cgatcgacgc cgtctacacc
     5941 gccgagcgcg gcgccgtgga gaagagagtc ctcgaggcgc tccggtcggc gccggtcctc
     6001 gcggagacgg ggctcgcgcc ggtcgacctg cggctcctct tcgtccacgc gcccgacgac
     6061 gtgcacgggg ccttccgcga cattgcgtcg gccgccgagg accggacgac cgcccggaac
     6121 aaggcgctcg tcgaggcgga aggcgcgctc gggcaggcgc gcggcgaggt cgcccggacc
     6181 ctcgccgacg ccgactcgga gcgaacgcgc ctcgtcgagc acgcgaaggg ggacgccgcc
     6241 gccttcgtcc cgctcgcgcg cgaggtcgcc gcggcgccgc ggacgagccg cctgcgcctg
     6301 aagctcgagg cgctcgagcg ggtcctgccg ggcgtcccga agctgatccg ccccgacccg
     6361 cgccgcgctc ccggcttcga gctctgggtg acgcctgcct cgccggccga tgccgcgtcg
     6421 gcgcccgctc cgatgaaggg gctggccggt ttcgtgagtc cgatccccgc gccgcccccc
     6481 gacgtccccg cggcggagcg ctgaggtgaa cgccatggaa gtacccctcg agccccggcc
     6541 atccgctcga cgccgccgcg tcgcgacggt ggcggcaggc gcggccctcc tcctcctggt
     6601 cgcacgggtg ccgtacgtcg tcgaggcgac ggagtacgcg gtgcggaccc gcttcggccg
     6661 tcccgtcgcc gtcgtgacgg agccgggcct ccacgcccgg gtgccgctcg tcgacgacgt
     6721 cgcccggctc gacgcccgcc tcctctactt cgatccgccg gcgggggagt tcctgacgaa
     6781 cgacaagaag aacatcgtcg tctctccggt cgtcctctgg cgaatcgaga agccgctgaa
     6841 gttcctccag acgctcgtca cgcgcgagag cgccgaggcg cgactcgccg acctcgtcgc
     6901 ctccgagacg ggagccgcgc tcggagccgt cccgttcacg cgcctcgtct cggcgacgga
     6961 cggcgaggcc gacctggcgg gcgtcgtcgc ggggatcgcc gagcgggttc gggcgcgcgc
     7021 cgccgcggac tacggcgtga aggtcgtcga cgtccgcctg aagaggatcg cgttcccgga
     7081 gcagaacctc ctctccgtct tcggccggat gaggtccgag agggagcgga tcgcccggcg
     7141 gttccgctcg gaaggggagg aggcggcgct gaagatccgc gccgagaccg accgcgagaa
     7201 gacccggctc ctctccgagg cgtaccggaa ggccgaggag gagaaggggg ccggcgaggc
     7261 cgaggccgcc cggatctacg cgaaggcgac gaaccaggac ccggacctct acaagttcct
     7321 ccgaacgctc cgcgcctacg agacgatcct cgacgagaag acgacgctcg tcctcccggg
     7381 cgattccgac gtcctgcgcc tcctgaccga cggcgccgcg ccgtcgccga agaaggggcc
     7441 gaattgaccc ggacggcgcg acgggcgctc tttgccgcct cggggctcgc cttcggggcc
     7501 tggctcctcg cgggcgtttc ggtcctccga ccggcgcagc agggggtcgt cctgcggctc
     7561 ggcgcggtcc agccgctccc gctcggcccc ggaatccact ggcggccggc ggcgatcgac
     7621 cgcgtcgtga aggtcgaggt gacgaggacc gagaccgtgc cgatcggctt ccggaccgcc
     7681 gacggcgggc gcgcgatgcc gctcccggcc gaggaggggc agtggctgac cggcgacacg
     7741 aacattctcg ccgttcagct cgcggtgcag taccgtgtcg cccggccggt cgaatgggtc
     7801 gtccacgggc gcgacacggc ggaggtcgtt cgccgggctg cggagtccgt cgtgaccgaa
     7861 tcgatgggtg tcctcccggt ggacgacgcg ctgacgggtg gccgtcacac gctcctcgaa
     7921 gaggttcgcc gcgggaccca ggagctcctc gaccggtggc agagcggtgt cgccgtcgtc
     7981 tccgtcgccc tccgttccgt cgacccgccc ccgcccgtcc tcgccgcgtt ccaggacgtg
     8041 caggacgcga agagcgaccg ggagcggttc gtgaacgagg cggagagcta cgcgaacgac
     8101 accctgccgc gggcccgcgg cgaggcggaa gcgctcgtct ccgcggccgc cagccagaga
     8161 cagcggcggg tcgaggaggc gcacggcgac gccgcccggt tcgacgcgct gcgccgggag
     8221 gcgagcctcg cccccgggcc gctccgcgcg cggctgtacc tcgagaccct cgagagggtc
     8281 ctgccccgga tgcgcctcta cgtcgtggac gaagcgaccc ggctcaggct cgtgaagccc
     8341 gagccggcga cccccggggc cgtgcccggc ggctgaaggc cgggcacggc cggcctcagc
     8401 cctggtaggc cttccggatg ttcgggatca ggtactcctg gaaggcgcgg tcgaggtcgt
     8461 agcggggcga gaggccccag tccttccggg cggcggagtc gtcgacgtcg gccggccagg
     8521 agtcgacgat cccctgccgc ttcgcgtcga ccttcgtcgt gatctccgcg ccggggaagg
     8581 cctgctcgac gatcgcctgg atctcggcgg ccgaaggggc gaaggccccg atgttgtacg
     8641 tcgtccgcgt cagcttctcg cgcggggccg cggcgagctg gaggagcgcg tcgacggcgt
     8701 cgggcatcgc catgaagggg atgcgcgcgt cgggcctcac gaagcaggcg tagggcttcc
     8761 cctgcgcggc cgcgtggatc atctcgggcg cgaagtcgga cgtgccgccc gacgggacgg
     8821 tgaacgccga gatgaggccg gggaagcgga tcgagcggaa gtccaccttc cccgagagcg
     8881 tttcgggagc gagctgcttg tagtggcggg cgtagtagcg gccgagctgc tcgcagtaga
     8941 gcttgttgca gccgtacatc gtcgtcgggt tcgtgaagtc gtcctccttc accctcccgg
     9001 ccttcgcctt cacgtcgagc cccggcattc cgtaggccgc gatcgacgac gggtacatga
     9061 agacgaccgg ccgcccgtgc gactcgccct gcttctgggc gaactcgagc atcgccatcg
     9121 tcccctcgac gttcacctgg tgcgccgtga ccggggtgaa ctcgctccgc gtggagagga
     9181 gcgcggcgag gtggaagacc tggtcgacct cgaactcgga gaggatccgc tcgaggagct
     9241 gcggctcgag gatcgagccg gtgaactgtc gcgccacgcg aggcgcgatc gacgggtcga
     9301 ggggcttcag gtcgagcgtg atgatcggac ggctcccgtc ctccgagagc cgctcgatga
     9361 gcgtgtggcc gatctctccg ctggctccag tgaggaagac gacgggtgtg cgcagcacga
     9421 aggcctcctt
//
LOCUS       DAOTEZ010000146         9352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5213_length_9352_cov_6.229752, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000146 DAOTEZ010000000
VERSION     DAOTEZ010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9352
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5213_length_9352_cov_6.229752"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..810)
                     /locus_tag="P5164_09180"
     CDS             complement(214..810)
                     /locus_tag="P5164_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HRY44105.1"
                     /translation="MQITDILAQMGGLQSMARELGISESQVAKGAEALAPAILGGFKK
                     QAQAQPTGLEGLGGLLGQLGGGGLLDEVLSPQPTNVNLGNDVLGQIFGSKDVSRAVAQ
                     NASAKSGLDPSILKKMLPMLAMLVTGFMAKQGGGGGAHASAGGGLGDLLGGLMGGGAS
                     RSAAPAAGASGGLGSLLDLDGDGNALDDILGMAGKLMR"
     gene            complement(846..1259)
                     /locus_tag="P5164_09185"
     CDS             complement(846..1259)
                     /locus_tag="P5164_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HRY44106.1"
                     /translation="MGLFGSILAKLGFGKPEPAPAPAPAPAPLPVPPVAAPAPAPVEP
                     PVPAPIAVVDVVAQLEQRAAANPQKLNWKTSIVDLLKLLELDSSFAARKELAVELGCP
                     ADFMGDSAKMNVWLHKTVLAKIAENGGNVPKDLLD"
     gene            1533..3644
                     /locus_tag="P5164_09190"
                     /pseudo
     CDS             1533..3644
                     /locus_tag="P5164_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010856480.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
     gene            complement(3689..4156)
                     /locus_tag="P5164_09195"
     CDS             complement(3689..4156)
                     /locus_tag="P5164_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44107.1"
                     /translation="MRFRRLVAFHEAGRTVIAWDAGLRLPRISIYPEEHTRGRILAGR
                     DFPDGVDEVDRRDQGVYLRLLASGPIAVVMAGGKLPNDPHVGIEIDTRRIAVFAHRAG
                     LEIGSVFHETAVRLAYLWEPVEALVNTLLMRETVSGALAARIIRATHPGPALE"
     gene            4292..5104
                     /locus_tag="P5164_09200"
     CDS             4292..5104
                     /locus_tag="P5164_09200"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY44108.1"
                     /translation="MSTPASPPVGRLPLSLRLPEGRLEAVLLRPGETDSGRAPVVFLH
                     DALGAISLWRDFPERLCSATRRDGLVYDRLGYGRSDPWPVSPGLDFFDVESGQSLPEV
                     LRQAGIERPVLFGHSDGASIALLFAAAFPDVPAAVVAVAGHVFIEEETVAGVEEAAIA
                     WRTTDLPARLARHHGEKAGAVFRFWSETWRDPAFRGFSAVEAIRPLRCPLLVLQGAKD
                     EYASSAQVEAIASAVAGPVRAVLLPGLGHFPHREDPERVIAETARFLEEHGA"
     gene            complement(5138..6325)
                     /locus_tag="P5164_09205"
     CDS             complement(5138..6325)
                     /locus_tag="P5164_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY44109.1"
                     /translation="MSDAKSLRIASRIVHAGQKTCPLTGAVATPIYQTSTFAFKDTDE
                     GAARFGGTDPGYKYTRLGNPTVAALEECVADLEGGCGAMAASTGMAAVHTVYFALLGA
                     GAHVVGTDALYGASRMILEKEYSRFGVTGSFVDTSDPANVEKAMRAETKLVYLETPAN
                     PTLKLCDIEAIAKIAHAHGALVCVDNTFATPLLQRPFELGADVVLHSMTKFLNGHSDV
                     VAGIVVAKDPELFKRLRSVHVNVGPTMDPHQAWLVFRGIKTLGLRLEKAQANALEVSR
                     FLEAHPKVAWVRHPFLPSHPQHELAKRQMSGGGAIVSFGVKGGLEAGRTLMNSMKLAT
                     LAVSLGGVETLIEHPASMTHASVPKAEREKAEISDDLVRIAIGCEDAADLCDDLAQAL
                     EKV"
     gene            6532..7473
                     /locus_tag="P5164_09210"
     CDS             6532..7473
                     /locus_tag="P5164_09210"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HRY44110.1"
                     /translation="MRVVESVVELIGRTPMVRLGALKEPGEADVYAKLEYLNPGMSVK
                     DRTALGLVRDAEERGLLKPGGTILEATAGNTGVGLALVGAARGYRVVIVMIEGYAREK
                     MVLIRGLGGEVVLVPHGSGIPGARAKVDELLATLPGAVAMRQFENPANARIHYETTAP
                     EVWEQLEGRIDGLAVGVGTGGTFTGIARFLKEKDPSIECWGVEPPDSIYGGGDGKGEH
                     MVEGIGNAFLPATFERSLADGVFTVPHAESYAMVDTLGRMGLPAGASSGAAVAGAKRL
                     ARRLGEGKRVVTLLTDPSERYFSKYAYDGLYEGRPIR"
     gene            7493..8845
                     /locus_tag="P5164_09215"
     CDS             7493..8845
                     /locus_tag="P5164_09215"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HRY44111.1"
                     /translation="MDYPIREFAMGGGILIAVVAILHVVVSHFAVGGGLVLAVLETAA
                     VRRDDRPMRDLVKRSSKILLLLSTVFGAISGVGIWFTIGVVHPAATSSLIHTFVWAWA
                     TEWAFFLLEVVTALAWVATWEKVSPRTHLLLIWLYAFAAFLSLVVIQGILGFMLTPGA
                     FASTASFFDGFLNPTYLPGILFRTGACLALAAAWMAFAALRERDRAARARLSRVLGAI
                     GALGLAVAAAGWIVWERALPGSVQHLFLGEKPLLAALATGRTHTFRVLLVAALLALLV
                     TALPRLQSWPIALLALVASAGVLAGYERVREGVRKPWVIAEQMFSNGVLLTELDTLNE
                     KGILSKAVWATKEAGGREVTMGEAVFRAECSSCHTRDGYLGIRKIASAMDADFAAMFL
                     TALRDDGANWKARAAGKDVRPDYPFMPPFVGTDEEIEALAGWLASLNAPQAAEVAGVR
                     "
     gene            8835..>9352
                     /locus_tag="P5164_09220"
     CDS             8835..>9352
                     /locus_tag="P5164_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44112.1"
                     /translation="MFAETSLPALDPMPIPGPPWLFHLLWLVTFAVHVVGVNVVLGSS
                     VLGTFAALDRDRAFGRPVARLFAEVNTWAIALTITFGIAPLLFVQVLYGRLFYSATVI
                     LAPAWLGLLLLLMAAYYLTYAAKYRLRAGKGAALPLALSALLFLAVAAIQVVIHLLSV
                     QPGRWEAALASP"
BASE COUNT         1299 a         3365 c         3255 g         1433 t
ORIGIN      
        1 ggacgtcacc ctttcaaggt gaagatcgcg ggttcgaatc ccgctggggg taccaacctt
       61 tcctgccgtc gagacgggcg tccctcgctc tcgcgcccac ggacgacacg cagcgagccg
      121 gcgcgttcct cccgttcgca cgaagagggg cgccggcccg gccgcccggt cgtgtcgcgc
      181 ggaaggcccg cgtcgcgggc cttccggcgg gattcagcgc atcagcttcc cggccattcc
      241 gaggatgtcg tcgagggcgt tcccgtcgcc gtcgaggtcg aggagcgagc caaggccgcc
      301 cgaagccccg gcggccgggg ccgcggaacg gctcgcaccg ccccccatga gtccgccgag
      361 gaggtcgccg aggccgccgc ccgcggaggc gtgcgcccct cccccgcccc cctgcttcgc
      421 catgaagccg gtgacgagca tcgcgagcat cgggagcatc ttcttgagga tcgacgggtc
      481 gaggcccgac ttcgccgacg cgttctgcgc gacggcgcgg ctcacgtcct tcgagccgaa
      541 gatctggccg aggacgtcgt tgccgaggtt cacgttcgtc ggctgaggcg agaggacctc
      601 gtcgaggagc ccgccgccgc cgagctggcc gaggaggccg ccgagcccct cgagacccgt
      661 cggctgggcc tgggcctgct tcttgaaccc gccgaggatc gccggggcga gcgcttcggc
      721 ccccttcgcg acctgcgact cgctgatgcc gagctcgcgg gccatcgact ggaggccgcc
      781 catctgggcg aggatgtcgg tgatctgcat gcgctgtctc ctctcttccg tcgggcgtcc
      841 cgcgctcagt cgagcaggtc cttcgggacg ttgccgccgt tctcggcgat cttcgcgagg
      901 acggtcttgt gaagccagac gttcatcttc gccgagtcgc ccatgaagtc cgcggggcag
      961 ccgagctcga cggcgagctc cttgcgcgcg gcgaagctgc tgtcgagctc gaggagcttg
     1021 aggaggtcga cgatcgaggt cttccagttc agcttctgcg ggttcgccgc ggcgcgctgc
     1081 tcgagctggg cgacgacgtc gacgaccgcg atcggcgccg gcacgggcgg ctcgaccggg
     1141 gccggagccg gggccgcgac ggggggaacc gggagcggag ccggtgccgg ggcgggggcg
     1201 ggagccggct cgggcttgcc gaagccgagc ttcgcgagga tcgatccgaa gagtcccatc
     1261 attccctcct gagctgctgg gtgcggagac gggacctcgt cggacggacg aagacctgcg
     1321 ccgcggttcc gtccggtcac ggtaccgacg tgcaaccccg aaatcaacat gaacgctccc
     1381 gaagcggcgc gaggccctct cgcgcgacga cgcccgtccc cgcgaccgcg taagatgcag
     1441 ggtccgcccg gagcggggcc gcccggcccg gcctccggga ctcagggagc aatcgcatga
     1501 gaactaccat cgttgccctc gcggtcctcg cgatggcgct tccgggtctt tcccagaccc
     1561 cggccgaccc gaaggccgag acgaacccgt tcttcatgga gtgggcgacg ccgttcggga
     1621 tggcgccctt cgagaagatc cgctcggagc acttccttcc ggccttcgag gcggggatcg
     1681 ccgagcgccg caaggaggtc gaggcgatcg cgtcgaacgc cgccgctccg acgttcgcga
     1741 acaccgtcga ggcgctcgag aacggcggct ccgcactttc gaaggtcagc aacgtcttct
     1801 acacgctcgt cggagcggag acgaacgaag agctccaggc gatcacgaag aaggtcgctc
     1861 cctccctttc ggccctccgc gacgacgaga tgctcgacga gaagctcttc gcgcgcgtga
     1921 aggcggtctg ggagaagcgg gagacctcga gcctcacccc cgagcagaag aagctcgtgg
     1981 aggagacgta caaggacttc gtccgcggcg gcgcgaacct cgcgcccgag gcgcggaagc
     2041 ggttccgcga ggtcaaccag gagctctcgc tcctcggcat ccgcttcggc gacaacctcc
     2101 tgaaggagac gaacggctac aggctcgtga tcgagaagaa ggccgacctc gccggcctcc
     2161 cgccgggcgt cgtcgccgcg gccgccgacg ccgcgaaggc cgcgaagctg gacgggaagt
     2221 gggtcttcac gctccagtac ccgtccatct ggcccttcct gaccttctcc gagaaccgcg
     2281 agctccgccg gcagctcctg acggcctact cgacccggtg cgacaaggga gacgcttccg
     2341 acaacaaggc gctcctctcg aagatggcgg cgctccgcgc cgagcgggcg aagctcctcg
     2401 gcttcgcgac ctgggccgac ttcgtcctcg acgacaacat ggcgaagacg ccggctcggg
     2461 tctacgagct gctggaccgg gtctggaagc cggcgctcga ggtggcgaag gccgacgcga
     2521 aggagctcga gaaggccctt caggccgacg tgccgggcgc gaagctcgag ccgtgggact
     2581 ggcactacta cgccgagaga gtccgcaagg cgcggttcga cctcgacgac caggagctcc
     2641 gcccctactt cccgctcgac cgcgtccgcg agggcgcctt ctcggtcgcg aaccggctct
     2701 acgggatcac gttcaccgag cggaaggacg tcccgaagta ccacgccgag gtgagcacgt
     2761 acgaggtgaa ggacgccgac ggctcgctcc tcgcgctcta cacgacggac taccaccccc
     2821 gcgccggcaa gcgcggcggc gcctggtgcg gccggctgcg cggccagtgg gtgcaggacg
     2881 gcaaggacgt ccgcccgatc gtcacgaacg tcatgaactt cacgcgcccg gcgggcgacg
     2941 cgccggcgct cctctcgctc gacgaggtgg agacgctctt ccacgagttc ggccacgcgc
     3001 tccacggcat gctctcgcgc gtcacgtacc gctcgatggc gggcgtcccg cgcgacttcg
     3061 tcgagctccc ttcccagatc atggagaact gggcgctcga gccgacggtc ctcgcgggct
     3121 acgcgaagca ctggaagacg aacgagccga tcccggccgc gctcgtcgcg aaggtcgaga
     3181 ggtcgaagct ctacgggcag ggcttcgcga ccgtcgagta cacggcggcc tcgtacctcg
     3241 acatgaactg gcacaccctc gccgagccga aggtcgagga cgcgaccacg ttcgagaagg
     3301 ccgcgctcgc gaagatcggg ctcatccccg agatcctccc ccgctaaagg agcccctact
     3361 tcagccacat cttcggcggg ggcggcggct attccgccgg gtactacagc tacctctgga
     3421 gcgaggtcct cgactccgac gctttcgacg cgttcaagga gaaggggatc ttcgacccgg
     3481 cgacggcgac gtcgttccgc cgaaacgtcc tcgagaaggg ggggacggaa gacgcgatga
     3541 cgctctaccg ggcgttccgc ggccgcgagc cgtccgtcct tccgctcctc gtcaagcgcg
     3601 ggctcgcgcc ggccgcggcc gccgagcctc cggccacgaa gtagtccggc acggcgggga
     3661 ggggggaccg cccctctccc cgccggcgct actcgagcgc cgggcccgga tgcgtcgcgc
     3721 ggatgatccg ggccgcgagc gcgccggaga ccgtctccct catcaggaga gtgttcacga
     3781 gagcctcgac cggctcccag aggtaggcga ggcgcacggc cgtctcgtga aagacggagc
     3841 cgatctcgag ccccgcgcgg tgcgcgaaga cggcgatccg gcgggtgtcg atctcgatcc
     3901 ccacgtgcgg gtcgttcggg agcttcccgc cggccatgac gaccgcgatc ggtccggacg
     3961 cgaggaggcg gaggtagacc ccctggtcgc gtcgatcgac ctcgtcgacg ccgtccggga
     4021 aatcccgccc cgcaaggatc cggccccgcg tgtgctcctc gggatagatc gagatcctcg
     4081 ggaggcggag ccccgcgtcc caggcgatga cggtgcggcc ggcttcgtgg aatgcgacga
     4141 ggcgacggaa cctcaagtgg ccctcccctt ccagattaca ggttcgggag ccgaaaaggt
     4201 gcgacgggag atcagcctgc gcgctcctta cgggccgcgg gagggcctct ttccgttgtg
     4261 gggccgcccg cggccccaca gaatcgggac gatgtcgacg cctgcctcgc ctcccgtcgg
     4321 acgcctcccg ctctcccttc gcctcccaga gggccggctc gaggcggtcc tcctccgccc
     4381 cggggagacg gactccggcc gcgctcccgt cgtcttcctc cacgacgccc tcggagcgat
     4441 ctccctctgg cgcgacttcc ccgagcgcct ctgctcggcc acccgcaggg acgggctcgt
     4501 ctacgaccgc ctcggctacg gccgctccga tccgtggccc gtttcgcccg ggctcgactt
     4561 cttcgacgtc gagtcggggc agtcgctccc ggaggtcctc cgccaggccg gcatcgagcg
     4621 tcccgtcctc ttcggccaca gcgacggcgc gtcgatcgcg ctcctcttcg ccgcggcgtt
     4681 ccccgacgtc ccggccgccg tcgtcgcggt cgcggggcac gttttcatcg aggaggagac
     4741 ggtcgcgggc gtcgaggagg cggcgatcgc ctggcggacg acggacctcc cggcccggct
     4801 cgcccggcac cacggcgaga aggcgggagc cgtcttccga ttctggagcg agacgtggcg
     4861 cgaccccgcg ttccgaggct tctcggccgt ggaggcgatc cggcctcttc ggtgcccgct
     4921 cctcgtcctc cagggagcga aggacgagta cgcctcctcg gcccaggtcg aggcgatcgc
     4981 ctcggccgtc gcggggccgg tccgggcggt cctcctcccg gggctcggcc acttcccgca
     5041 ccgcgaggac ccggagcggg tgatcgccga gacggcgagg ttcctggaag agcacggggc
     5101 ctgaagcgcc ggcttcaggc cccggtgaga ccgggcttca gaccttctcg agcgcctggg
     5161 cgaggtcgtc gcagaggtcg gcggcgtcct cgcaaccgat cgcgatcctc acgaggtcgt
     5221 cggagatctc ggccttctcg cgctcggcct tcggcacgct cgcgtgcgtc atcgacgccg
     5281 ggtgctcgat gagcgtctcg acgcccccga ggctgacggc gagcgtcgcg agcttcatcg
     5341 agttcatcag agtccgcccc gcctcgagcc cgcccttcac cccgaaggag acgatcgcgc
     5401 cgccgcccga catctggcgc ttcgcgagct cgtgctgcgg gtggctcggg aggaacgggt
     5461 ggcggaccca ggcgaccttc gggtgggcct cgaggaaccg ggagacctcg agggcgttgg
     5521 cctgggcctt ctcgaggcgc aggccgagcg tcttgatccc gcggaagacg agccacgcct
     5581 ggtgcgggtc catcgtcggt ccgacgttca cgtggaccga gcgaagccgc ttgaagagct
     5641 ccgggtcctt cgcgacgacg atcccggcca cgacgtcgct gtggccgttc aggaacttcg
     5701 tcatgctgtg gaggacgacg tcggccccga gctcgaacgg ccgctggagg agcggcgtgg
     5761 cgaacgtgtt gtcgacgcag acgagggcgc cgtgggcgtg ggcgatcttc gcgatcgcct
     5821 cgatgtcgca gagcttgagc gtcgggttcg cgggcgtctc gaggtagacg agcttcgtct
     5881 cggcgcgcat cgccttctcg acgttcgccg ggtcggacgt gtcgacgaag gagcccgtga
     5941 cgccgaaccg ggagtattcc ttctcgagga tcatccggct cgcgccgtag agagcgtccg
     6001 tcccgacgac gtgggccccc gccccgagga gggcgaagta gacggtgtgg acggcggcca
     6061 tgcccgtcga ggcggccatc gccccgcagc ccccttccag gtcggcgacg cactcctcga
     6121 gggccgcgac cgtggggttg ccgaggcgcg tgtacttgta gcccgggtcg gtgccgccga
     6181 agcgggcggc gccctcgtcc gtgtccttga acgcgaacgt cgaggtctgg tagatcggcg
     6241 tcgcgacggc tcccgtgagc gggcaggtct tctgcccggc gtggacgatc cggctggcga
     6301 tgcgcaggct cttggcgtcg gacattctgt ggagcacctc tcgaggagtg gcggaccgga
     6361 ggggatgcgg ccgcccagcc gggcatccta gcatatgctc gattccggag cgccccggcg
     6421 aggccggcgg cgtccccttc gcattccttg acttcccctc ctcccccgcc tacaagaacc
     6481 gctgggctct cgccggggcg cgccgcgccc gaagggcccc gggagcggtg catgcgtgtc
     6541 gtggaaagcg tcgtcgagct gatcgggcgg accccgatgg tgcggctcgg cgccctgaag
     6601 gagcccggcg aggcggacgt ctacgccaag ctcgagtacc tgaaccccgg gatgagcgtg
     6661 aaggaccgga cggctctcgg cctggtccgc gacgccgagg agcgcgggct cctgaagccc
     6721 ggcggaacga tcctcgaggc gaccgcgggg aacaccggag tcggcctcgc cctcgtcggc
     6781 gccgcgcgcg gctaccgggt cgtcatcgtg atgatcgaag ggtacgcgcg cgagaagatg
     6841 gtcctgatcc gggggctcgg cggcgaggtg gtcctcgtgc cgcacggctc cgggatcccc
     6901 ggcgcgcgcg cgaaggtgga cgagctcctt gcgacgctcc ccggcgccgt cgcgatgcgg
     6961 cagttcgaga acccggcgaa cgcccggatc cactacgaga cgaccgcgcc cgaagtctgg
     7021 gagcagctcg agggccgcat cgacggcctc gcggtcggcg tcggcacggg cgggacgttc
     7081 accgggatcg cccgcttcct gaaggagaag gacccgtcga tcgagtgctg gggcgtcgag
     7141 cccccggact cgatctacgg cggaggcgac gggaagggcg agcacatggt cgaggggatc
     7201 gggaacgcct tcctcccggc cacgttcgag cggtccctcg ccgacggcgt cttcacggtc
     7261 cctcacgccg agagctacgc gatggtcgac accctggggc ggatggggct cccggcggga
     7321 gcctcctcgg gagccgccgt cgccggggcg aagcgcctcg cgagaaggct cggcgagggg
     7381 aagcgcgtcg tcacgctcct gaccgacccg tcggagcgct acttctcgaa gtacgcctac
     7441 gacgggctct acgaggggcg accgatccgc tgaggcaggc gaggagaccg ggatggacta
     7501 tccgatccgg gaattcgcga tggggggcgg catcctgatc gcggtcgtcg cgatcctcca
     7561 cgtcgtcgtc tcgcacttcg ccgtcggggg cggcctcgtc ctcgccgttc tcgagacggc
     7621 ggccgtccgg cgcgacgacc gtccgatgcg cgacctcgtg aagcgctcgt cgaagatcct
     7681 gctcctcctc tcgaccgtct tcggggcgat ctcgggggtc ggcatctggt tcacgatcgg
     7741 cgtcgtccac cccgcggcga cctcgtcgct catccacacg ttcgtctggg cctgggccac
     7801 ggagtgggcc ttcttcctcc tcgaggtcgt caccgcgctc gcctgggtcg cgacgtggga
     7861 gaaggtctcg ccgcggactc atctcctcct catctggctc tacgcgttcg ccgccttcct
     7921 gtcgctcgtc gtgatccagg ggatcctcgg cttcatgctg acgcccgggg ccttcgcgag
     7981 cacggcgtcg ttcttcgacg gattcctgaa cccgacgtac ctgccgggga tcctcttccg
     8041 caccggcgcc tgcctcgccc tcgccgccgc ctggatggct ttcgccgccc tgcgggagag
     8101 ggaccgcgcg gcgcgcgccc gcctctcccg ggtcctcggc gcgatcgggg ctctcggcct
     8161 cgcggtcgcg gccgccggct ggatcgtctg ggagagggcg ctccccggtt cggtccagca
     8221 cctcttcctc ggtgagaagc cgctcctcgc cgccctcgcc acgggacgga cgcacacgtt
     8281 ccgtgtcctc ctcgtcgccg ctctcctggc gcttctcgtc acggcgctcc cccgccttca
     8341 gtcgtggcca atcgccctcc tcgcgctcgt cgcctcggca ggcgtcctcg ccgggtacga
     8401 aagggtccgc gaaggggtcc gcaagccctg ggtgatcgcc gagcagatgt tctcgaacgg
     8461 ggtcctcctg acggagctcg acacgctgaa cgagaaaggg atcctctcga aggccgtctg
     8521 ggcgacgaag gaggcgggcg ggcgcgaggt gacgatgggc gaagcggtct tccgcgccga
     8581 gtgctcctcc tgccacacgc gcgacggcta cctcggcatc cggaagatcg cctcggcgat
     8641 ggacgccgat ttcgccgcga tgttcctgac ggcccttcgg gacgacggcg cgaactggaa
     8701 ggcccgcgcc gcagggaaag acgtgaggcc cgactacccg ttcatgccgc cgttcgtcgg
     8761 gaccgacgaa gagatcgagg cgctcgccgg atggctcgcc tccctgaacg cgccgcaggc
     8821 ggcggaggtg gccggtgttc gctgagacct cgctcccggc gctcgacccg atgccgatcc
     8881 cggggccgcc gtggctcttc cacctcctct ggctcgtcac gttcgccgtg cacgtcgtcg
     8941 gcgtgaacgt cgtcctcggc tcctcggtcc tcggcacctt cgccgccctg gaccgtgacc
     9001 gcgccttcgg gcgccccgtc gcccgcctct tcgccgaggt caacacctgg gcaatcgcgc
     9061 tgacgatcac gttcgggatc gcccctctcc tcttcgtcca ggtcctctat ggccggctct
     9121 tctactccgc gaccgtgatc ctcgccccgg cctggctcgg cctcctcctc ctcctgatgg
     9181 cggcctacta cctgacctac gccgcgaagt accgcctccg ggccgggaaa ggcgccgcgc
     9241 tccccctcgc tctctcggcc ctcctcttcc tcgccgtcgc ggcgatccag gtcgtcatcc
     9301 acctcctctc ggtccagccc ggccgctggg aggcggcgct cgcgagtccc tg
//
LOCUS       DAOTEZ010000147         9303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5255_length_9303_cov_5.832396, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000147 DAOTEZ010000000
VERSION     DAOTEZ010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9303
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5255_length_9303_cov_5.832396"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="P5164_09225"
     CDS             <1..433
                     /locus_tag="P5164_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HRY44113.1"
                     /translation="VGSGWVASKDALFPPAAIPGPVRLATLSEIPVGGMTQFTIPGLD
                     QPGILIRLEEGLFRAFEQKCTHLSCAVFFDPGSRKIECPCHNGAFDPISGAVLQGPPP
                     RPLRSFPVEVRGDEVWLVPPEAPAEAAAPAGPPAAAGQEGV"
     gene            430..1962
                     /locus_tag="P5164_09230"
     CDS             430..1962
                     /locus_tag="P5164_09230"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY44114.1"
                     /translation="MTDFRRAQEQSHPSKTNTVLLGVILLLVLIVSLQVWLLTAGLNT
                     ALGGDRSIVWPAFQASLVLFLAGAASLRYLPRPIRAVAARPAAEAFPDAALAWRTLAI
                     SAVSLTLAFSVWFMWSAIAIKLPDAGFRISPSQRFWLTATPVILGSLLRIPYGVFVSR
                     FGSRRSLAAVTLLLLVPCIGTGIAVQDPTTPFGVLLFWSAVTGIAGANFATSMAVVTL
                     WFPKSKQGSALGINGLGNLGVTVAQLLVPVVIAAGLFGALGGGSLSLRLKAGGTQAVW
                     LQNAAFVWIPVILLCTAAVWFGTRDFPMKPKTIASQLGVARDLHTWTISALYFLTFGC
                     FVAMGASLPLIIQSVFADAPGGAPSPLLYAPFAPLVATLLRPVGGWAADRFGAGRMTA
                     IAIGVMAVGGFSLSRFLRPDSFDGFFATILVICAASGFGNGSVFKIIPTVNPREAGAV
                     IGIVSCVGAFGGFFPPLFLGWCIERFGSPAWAYTAMAVVALGALAVNGWFYQREGSPT
                     RC"
     gene            2104..2394
                     /locus_tag="P5164_09235"
     CDS             2104..2394
                     /locus_tag="P5164_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44115.1"
                     /translation="MKVPGRMTSLVVSCLPACAAAAEVLPEAPRSAGNVGVSGLWVLA
                     NICFAGMRAQNSPRAGWRIVAFLFGFPGTLVSYFVVAEGSERAWGVEIPRKR"
     gene            2516..2971
                     /locus_tag="P5164_09240"
     CDS             2516..2971
                     /locus_tag="P5164_09240"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRY44116.1"
                     /translation="MRSPLTVLAFCALGLAGCATRQAVSPLDSGIGVSLDDYLRSLNE
                     ADPSDPVVSERDPSVKARLTPPVELRRVQPALSREARRNSREGKVVLGCVIERNGSVS
                     NVRVISASGHGDFTAASVGAVRQWRYSPAKLDGSPVRVFLTVTTSYRVR"
     gene            complement(3027..3605)
                     /locus_tag="P5164_09245"
     CDS             complement(3027..3605)
                     /locus_tag="P5164_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44117.1"
                     /translation="MRRAVIGLLAALFAGACGGGPTPEQLATKAALVDAHVARAAASH
                     LASRYDEAAAVQPPAEEGTAPSWEAAAAYRASAEGLAAVTYLSELGKPVSVDPSFPRA
                     GDIDAVRRATTELVTIVLQPAGTREEFAARVSDAQARLFSAVSALEKGTKNHILIETR
                     SESNIRMAEYTEILAGSRAAGAGTAPGASPTP"
     gene            3709..4254
                     /locus_tag="P5164_09250"
     CDS             3709..4254
                     /locus_tag="P5164_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007426629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRY44118.1"
                     /translation="MPGRPALLAAVLALAVPLRADPPPPSPVEAPRIVVRKARRELLL
                     FSGETLLRTYRVGLGFNPVPPKERQGDGATPEGRYFVCRKNPRSQFLLSLGLSYPNAA
                     DADRGLAAGLVTRGQHRRIVEAERRGACPPWDTRLGGEIFIHGRGSSSDWTWGCVALD
                     DDDVRELYARIPVGTPVVVEP"
     gene            4305..4698
                     /locus_tag="P5164_09255"
                     /pseudo
     CDS             4305..4698
                     /locus_tag="P5164_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017981787.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
     gene            complement(4671..5489)
                     /locus_tag="P5164_09260"
     CDS             complement(4671..5489)
                     /locus_tag="P5164_09260"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HRY44119.1"
                     /translation="MSADPSREQVLALLDRLERGELRAAEPDGDGWRVNPEVKEGILR
                     AFRLGVDVDLPATPFAFRDRDTLPPRGEVPAGVRVVPGGTAIRRGAFVAPGVVVMPPA
                     YVNVGAYVGEGTMIDSHALVGSCAQVGRRVHLSAAAQIGGVLEPVGALPVVVEDDVFV
                     GGGCGLYEGVRVRTGAVLAAGVVLTGSGVLYDLVREREIRATEGEPLTVPAGAVVVPG
                     SRPAPGSWAHVRGLSISAAVIVKYRDARTAARTALEEALRPGLDAAQSPGSVRT"
     gene            complement(5486..6430)
                     /gene="dapA"
                     /locus_tag="P5164_09265"
     CDS             complement(5486..6430)
                     /gene="dapA"
                     /locus_tag="P5164_09265"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRY44120.1"
                     /translation="MKTQEVPRIHLTGAFTALVTPFTEKGEVDEPALRKLVRRQVAGG
                     VAGVVPCGTTGEAVTLEPDEHERVVAVTVEEARTARRPIRVIAGCGSNDTRRSQKLAE
                     RCRRAGADALLVVTPYYNKPTPRGLIEHFRAVADAGHLPVVAYNVPARTGVNMLPETV
                     LQLAEDPRMCAVKEASGSLDQACEILRHRPAGFSVLSGEDSLALPMISCGADGVIAVV
                     SNEAPALLVALVEAALTGERDEAARLQARLLPLMRANFRESNPIPVKWALQRLGLIQD
                     VLRLPLVPLSKGLHRPMEEALKEAGLLDDAGNVAEEGP"
     gene            complement(6427..7113)
                     /locus_tag="P5164_09270"
     CDS             complement(6427..7113)
                     /locus_tag="P5164_09270"
                     /inference="COORDINATES: protein motif:HMM:NF017024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HRY44121.1"
                     /translation="MKALIVGPGKMGREIEAVLLRRGHGVRARYGRRDDLESLEPDVL
                     DVAFEFTAPESAPGTVATLLMHRVAVVSGTTGWDVEPARRLAEERKVPFLHSPSFSIG
                     VAALRRAAMVLGGALARFPEFEPAIVERHHSAKRDAPSGTAKAISEAVGVGRSAGEEI
                     PIVSLRHGGQPGEHVLVFEGPDESIELVHRARSRSVFALGAVRAAEWLVKSGLTGPVT
                     FDDFLDRRTR"
     gene            complement(7110..8474)
                     /locus_tag="P5164_09275"
     CDS             complement(7110..8474)
                     /locus_tag="P5164_09275"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRY44122.1"
                     /translation="MKLGGTSVGGADRIEAAASLVASRLDEGPFVVVSAMAGVTDTLL
                     ALSRAAREGRAEEVRAALQEIVARHREAIDALGLGAGATAALQEEVAREAARLESLAT
                     GVGLLGELSARTSDAICAAGELLSSALVAAALERRGVAAVRVDPRDWMATDGTHGAAL
                     PDEAALARNAAARLLPERAAGRVVVTGGFVGAGPDGAATTLGRGGSDFSAALLGAALR
                     DAGADVSAIEIWTDVDGILTSDPRIVAGARPVPLVGYAEAAELAFFGAKVLHPATIRP
                     AVARGIPVRVRNTFRPGAAGTTIRAEAPGEGVRALAMRTGVAALFVGSPRMLLAHGFA
                     ARVFSVFERRGVAVDVIATSEVSISITVDARAPIALLVRDLEELGEVSVLADLAVVSV
                     VGRRLRSTPRVAARVFGALGDLNVVLISQGASETNLTFVVEAKDGPEALRRLHRELFE
                     KEAA"
     gene            complement(8557..>9303)
                     /gene="asd"
                     /locus_tag="P5164_09280"
     CDS             complement(8557..>9303)
                     /gene="asd"
                     /locus_tag="P5164_09280"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRY44123.1"
                     /translation="LVVSNTKSFRMQADVPLLIPELNADHLALLDRQEWAKAGGGIVT
                     NPNCVVVGLAMALAPLHRAFGVEAVCVTTLQALSGAGYPGVPSLDAIGNVVPFIDGEE
                     EKVASEPGKILGALAGGRVEPASFPVSVSVNRVPVRDGHTESVFVKLGRKTTLAEVRE
                     ALETFVGEPQRLGLPSAPARPIVVRDERDRPQPIRDVDAGGGMVVTVGRLRPDPVYDV
                     RFTLLVHNTVRGAAGAALLNAELLAATGRL"
BASE COUNT         1152 a         3584 c         3266 g         1301 t
ORIGIN      
        1 cgtggggagc ggctgggtcg cgtcgaagga cgccctcttc ccccccgcgg cgatccccgg
       61 tccggtccgg ctcgcgacgc tctccgagat cccggtcggc gggatgacgc agttcacgat
      121 cccgggcctc gaccagccgg ggatcctgat ccgcctcgag gaggggctct tccgcgcctt
      181 cgagcagaag tgcacccacc tctcctgcgc ggtcttcttc gaccccggat cgcggaagat
      241 cgagtgcccc tgccacaacg gggcgttcga cccgatcagc ggcgccgtcc tccaggggcc
      301 gccgccgcgg ccgctccgct ccttccccgt cgaggtccgt ggcgacgaag tctggctcgt
      361 gcctcccgag gccccggccg aggccgctgc tccggccggc ccgccggccg cggccggaca
      421 ggaaggcgta tgaccgactt ccgccgcgcc caggagcagt cgcacccgtc gaagacgaac
      481 accgtccttc tcggggtcat cctcctcctc gtcctcatcg tctcgctcca ggtctggctc
      541 ctgacggcgg gcctcaacac ggccctcggc ggcgaccggt cgatcgtctg gcccgccttc
      601 caggcctcgc tcgtcctctt cctcgccggg gcggcctcgc tccgctacct gccgcgaccg
      661 atccgcgccg tcgcggcgcg ccccgcggcg gaggccttcc ccgacgcggc gctcgcctgg
      721 cggacgctcg cgatcagcgc cgtctccctc acgctcgcct tctccgtctg gttcatgtgg
      781 tcggcgatcg cgatcaagct ccccgacgcc ggcttccgga tctcgccgtc gcagcgcttc
      841 tggctgacgg cgacgccggt catcctcggg tcgctcctcc gcatccccta cggcgtcttc
      901 gtctcgcgct tcggcagccg gcggtcgctc gcggccgtca cgctcctcct cctcgtcccc
      961 tgcatcggga cggggatcgc ggtgcaggac ccgacgacgc ccttcggcgt cctcctcttc
     1021 tggtcggccg tcaccggcat cgccggggcg aacttcgcga cctccatggc cgtcgtcacg
     1081 ctctggttcc cgaagtcgaa acagggctcg gcgctcggga tcaacggcct cgggaacctc
     1141 ggcgtcaccg tcgcgcagct cctcgtcccg gtcgtgatcg cggccggcct cttcggcgcc
     1201 ctcggcggcg ggtcgctctc cctccggctg aaggcgggcg ggacgcaggc cgtctggctc
     1261 cagaacgccg ccttcgtctg gatcccggtc atcctcctct gcacggcggc ggtctggttc
     1321 gggactcgcg acttcccgat gaagccgaag acgatcgcct cgcagctcgg cgtcgcgcgc
     1381 gacctccaca cctggacgat ctcggcgctc tacttcctca cgttcggctg cttcgtggcg
     1441 atgggggcct cgctcccgct catcatccag tcggtcttcg ccgacgctcc gggcggcgcc
     1501 ccgagcccgc tcctctacgc tcccttcgcc ccgctcgtcg cgacgctcct gcgccccgtc
     1561 ggcggctggg ccgcggaccg tttcggcgcc ggcagaatga cggcgatcgc gatcggcgtg
     1621 atggccgtcg gaggcttcag cctgagccgc ttcctccgcc ccgactcctt cgacggcttc
     1681 ttcgcgacga tcctcgtcat ctgcgcggcg tccggcttcg ggaacggctc ggtcttcaag
     1741 atcatcccga ccgtcaaccc gcgcgaggcc ggcgccgtca tcgggatcgt ctcgtgcgtc
     1801 ggcgccttcg gcggcttctt cccgcccctc ttcctcggct ggtgcatcga gcggttcggg
     1861 agccccgcct gggcctacac ggcgatggcc gtcgtcgccc tcggcgccct cgccgtcaac
     1921 ggctggttct atcagcggga ggggtcgccc acgcgctgct gagacggacc gggaccaccg
     1981 ggctccggca ggatcgcaac ttcccattct tcgttcccgt ccccgttcga gacggacgtc
     2041 ccgggcgtgg gagagaatcg gcgccggtct ccacgaccgg agctcgaatc gggaggagga
     2101 cgaatgaagg taccgggtcg aatgacgtcg ctcgtcgtct cctgcctgcc cgcgtgcgcc
     2161 gcagcggcag aggtcctgcc ggaggcgccg aggtcggccg ggaacgtcgg ggtgtcgggc
     2221 ctctgggtcc tcgcgaacat ctgcttcgcc ggcatgaggg cgcagaacag cccgcgtgcc
     2281 gggtggcgga tcgtcgcctt cctcttcggg tttcccggca ccctcgtgag ctacttcgtc
     2341 gttgccgagg gaagcgaacg cgcctggggc gtcgagattc ccaggaagcg atgagcctcg
     2401 caggccggga aaccatgccg gcggcgcccg gggacgagag agagaagtgg cccgtcgccc
     2461 tctccttggc tcgcgtctcc cggccgccca cgatcacgaa ggcaaaggag ccccgatgcg
     2521 ctcgcccctg accgttctcg cgttctgcgc tctcgggctc gccggctgcg cgacccggca
     2581 ggccgtctct ccgctcgact ccggtatcgg cgtctccctg gacgactacc tgcgcagcct
     2641 caacgaggcc gatccctccg accccgtcgt cagcgaacgg gacccctcgg tgaaggcgcg
     2701 cctgacgccc ccggtcgagc ttcgtcgcgt ccaacccgcg ctcagccggg aggcgcgcag
     2761 gaactcccgc gaggggaagg tcgtcctcgg gtgcgtcatc gagcgcaacg gctcggtctc
     2821 gaacgtccgg gtgatctccg cctccggcca cggcgacttc acggccgcct ccgtcggggc
     2881 cgtgcgccag tggcgctact cccccgcgaa gctggacggc tcgcccgtga gggtcttcct
     2941 caccgtcacc acctcgtatc gcgtccggta ggccagccgt cggcgcggcg ccggccggct
     3001 ggcgccagaa cggtctgccc ggcccgtcag ggggtcgggg acgccccggg ggccgttccg
     3061 gccccggccg ccctggagcc cgcgaggatc tcggtgtact cggccatccg gatgttcgac
     3121 tcggagcgcg tctcgatgag gatgtggttc ttcgtcccct tctcgagcgc cgagacggcg
     3181 ctgaagagcc gggcctgcgc gtcggagacc cgggccgcga actcctcccg ggtgccggcc
     3241 ggctgaagga cgatcgtcac gagctccgtc gtggcccggc ggacggcgtc gatgtcgccg
     3301 gcgcgcggga aggacgggtc gacggatacg ggcttcccga gctcggagag gtacgtgacc
     3361 gcggcgagcc cctccgccga cgctcggtag gcggcggccg cctcccagga cggggcggtt
     3421 ccctcctccg cgggaggctg gacggccgcg gcctcgtcgt agcgcgaggc gaggtggctg
     3481 gctgcagccc gggcgacgtg cgcgtcgacg agcgccgcct tcgtcgcgag ctgctcgggc
     3541 gtcggcccgc cgccgcacgc gcctgcgaaa agggccgcaa ggagtccgat caccgctctg
     3601 cgcatgggtg cctcccttcg agccggagcc gaaagcagcc gccggtgccc cggccccccg
     3661 tcgtccattc gcccgattct agggcagccg cgcgtgcgac gatgacccgt gcccggccgc
     3721 ccggcgctcc tcgcagccgt cctcgcgctc gccgtccccc tgcgggccga tccgcccccg
     3781 ccctctcccg tcgaggcgcc gaggatcgtc gtgcggaagg cgcgacgcga gctcctcctt
     3841 ttctccggcg agaccctcct ccggacctac cgcgtcgggc tcggcttcaa ccccgtcccg
     3901 ccgaaggaac ggcaggggga cggcgcgacg ccggaggggc gctacttcgt ctgccggaag
     3961 aacccgcgca gccagttcct cctctcgctc gggctgagct acccgaacgc cgcggacgcc
     4021 gaccgtggtc tcgccgccgg gctcgtcacg cgagggcagc accggaggat cgtcgaagcg
     4081 gagcggcgcg gggcgtgccc gccctgggac acgcggctcg gcggggagat cttcatccac
     4141 gggaggggct cctcgagcga ctggacgtgg ggctgcgtgg cgctcgacga cgacgacgtc
     4201 cgggagctct acgcccggat ccccgtcggg acgccggtcg tcgtcgaacc gtgacgccgg
     4261 ccgcgccggg ccggtcgcgc cgactctctg ccagactcgg acgcatgcgc tcgctccgct
     4321 actccgtcct tcccggctcg tgggccgtcg tccggttccc gcccgacgcc gaggtcccgg
     4381 ccgccctcct cgtcccgccg ttccactccg tgacgcggac ggcggtcgag ctgtcggtcg
     4441 tctgcccgga cgggacggtg ccggcggggg ctcgggccga gcggggatgg gcggtcctcg
     4501 ctctcgaggg tccgttcccc ttcgacctcg tcggcgtcct cgccgcgttt cctcgtgccg
     4561 ctcgccgagg ccggcgtcgg gatcttcgcc ctgagcacgt tcgacacgga ctacgtcctc
     4621 gtgaagcggg aacggctcga ggcggcgctc gaagccctct ccgccgccgg tcacgtgcgg
     4681 acggacccgg gagactgagc ggcgtcgagg cccggacgga gggcttcctc gagcgccgtc
     4741 cgcgccgccg tcctcgcgtc gcggtacttc acgatcaccg ccgccgagat cgagaggccg
     4801 cgcacgtgcg cccaggagcc gggcgccggc cgcgagcccg gaacgacgac ggctcccgcc
     4861 ggcacggtga gcggctctcc ctccgtcgcg cggatctccc gctctcggac gaggtcgtag
     4921 aggacgcccg agccggtcag gacgacgccc gccgcgagga cggcccccgt ccggacgcgc
     4981 accccctcgt agaggccgca gccccccccg acgaagacgt cgtcctcgac gacgaccggg
     5041 agcgcgccga cgggctccag gacgcctccg atctgcgccg cggccgagag gtggacgcgc
     5101 ctgcccacct gcgcgcagga tccgacgagg gcgtggctgt cgatcatcgt cccctcgccg
     5161 acgtacgccc cgacgttcac gtaggcgggc ggcatgacga cgacgccggg cgccacgaac
     5221 gcgccccgcc ggatcgccgt gcctcccggg acgacgcgga cgccggccgg cacctcgccg
     5281 cgcggcggca gcgtgtcgcg gtcgcggaac gcgaagggcg tcgcggggag gtcgacgtcg
     5341 acgccgaggc ggaaggcgcg gaggatcccc tccttcacct cggggttcac gcgccagccg
     5401 tcgccgtccg gctcggccgc ccgaagctcg ccgcgctcga ggcggtcgag gagggcgagg
     5461 acctgctcgc gcgaggggtc ggcgctcacg gcccctcctc cgccacgttc ccggcgtcgt
     5521 cgaggagtcc agcctccttc agcgcctcct ccatcggcct gtgcaggccc ttcgagagcg
     5581 ggacgagcgg cagccggagg acgtcctgga tcagcccgag ccgctggagc gcccacttca
     5641 ccgggatcgg gttcgactct cggaagttcg cgcgcatgag cggcaggagc ctcgcctgga
     5701 ggcgcgccgc ttcgtcccgc tcgcccgtga gcgccgcctc gacgagcgcg acgaggagcg
     5761 ccggcgcctc gttcgagacg acggcgatga cgccgtcggc cccgcaggag atcatcggga
     5821 gcgcgagcga gtcctccccc gagaggaccg agaagcccgc cggccggtgg cgcaggatct
     5881 cgcaggcctg gtcgaggctc ccggaggcct ccttcaccgc gcacattcgt gggtcttccg
     5941 cgagctggag gaccgtctcg gggagcatgt tcacgccggt gcgcgccggg acgttgtagg
     6001 cgacgacggg caggtgcccg gcgtcggcca cggcgcggaa gtgctcgatc aggccgcgcg
     6061 gcgtcggctt gttgtagtac ggcgtgacga cgaggagcgc gtcggccccc gccctccggc
     6121 accgctccgc cagcttctgc gaccgacgcg tgtcgttcga gccgcagccg gcgatcaccc
     6181 ggatggggcg gcgggccgtc cgcgcctcct cgaccgtgac cgccacgacc cgctcgtgct
     6241 cgtcgggctc gagcgtcacc gcctcccccg tcgtcccgca cggaacgacg cccgccacac
     6301 ccccggcgac ctggcgtcgc acgagcttcc ggagcgccgg ctcgtcgacc tcgcccttct
     6361 ccgtgaacgg cgtcacgagc gccgtgaagg ctcccgtgag gtggatgcgg ggcacctcct
     6421 gcgtcttcat cgggtcctcc tgtcgagaaa gtcgtcgaac gtgacgggcc ccgtgaggcc
     6481 cgacttcacg agccactcgg ccgcccggac ggcgccgagg gcgaagaccg atcgggaccg
     6541 cgcgcggtgg acgagctcga tcgactcgtc gggcccctcg aagacgagga cgtgctctcc
     6601 cggctgcccg ccgtggcgca gcgagacgat cgggatctcc tcgcctgcgg accggccgac
     6661 gcccacggcc tccgagatcg ccttcgccgt tcccgacggt gcgtccctct tggcggaatg
     6721 atgccgctcc acgatcgccg gctcgaactc ggggaagcgc gcgagcgccc cgccgaggac
     6781 catcgccgcc cgccgaagcg ccgcgacgcc gatggagaag ctcggcgagt ggaggaacgg
     6841 caccttccgc tcctccgcga gccggcgcgc cggctcgacg tcccagcccg tcgtccccga
     6901 gacgaccgcg acgcgatgca tcagcagtgt ggcgaccgtc cccggggccg attcgggtgc
     6961 ggtgaactcg aacgcgacgt cgaggacgtc gggctcgagc gactcgaggt cgtcgcgtct
     7021 cccgtatcgg gcgcggaccc cgtgcccgcg ccggaggagg accgcttcga tctcccgccc
     7081 catcttcccc ggtcccacga tgagagcctt cacgccgcct ccttctcgaa gagctcgcgg
     7141 tggagccgcc ggagcgcctc ggggccgtcc ttcgcctcga cgacgaacgt gaggttcgtc
     7201 tcggaggctc cctgcgagat caggaccacg ttcaggtcgc cgagggcccc gaagacccgt
     7261 gcggcgacgc gaggcgtcga gcggagccgc cgcccgacga ccgagacgac cgcgaggtcg
     7321 gcgaggaccg acacctcgcc gagctcctcg aggtcccgga cgagaagcgc gatcggggcg
     7381 cgagcgtcga cggtgatcga gatcgagacc tcgctcgtcg cgatgacgtc caccgcgacg
     7441 cctcgccgct cgaagacgga aaagacgcgc gccgcgaagc cgtgggcgag gagcatccgc
     7501 ggactgccga cgaagagcgc ggccacgccg gtgcgcatcg cgagcgcccg aacgccctcg
     7561 cccggcgcct ccgcccggat cgtcgtcccg gcggcgccgg gccggaacgt gttcctcacg
     7621 cgcaccggga tcccgcgcgc gacggcgggg cggatcgtcg ccgggtgaag gaccttcgca
     7681 ccgaagaagg ccagctcggc cgcctcggcg tagccgacga gagggaccgg acgcgcgccc
     7741 gcgacgatcc tcgggtcgga cgtcaggatg ccgtccacgt ccgtccagat ctcgatcgcg
     7801 gagacgtcgg ccccggcgtc ccgaagcgcc gccccgagga gagccgccga gaagtccgac
     7861 ccgccgcggc cgagcgtcgt cgcggccccg tcgggtccgg ctccgacgaa gcctcccgtt
     7921 acgacgacgc gtcccgcggc ccgctccggc aggagccgcg cggccgcgtt ccgggcgagg
     7981 gcggcctcgt cggggagcgc ggcgccgtga gtgccgtcgg tcgccatcca gtcgcgcggg
     8041 tcgacccgga cggccgcgac gccccgccgc tcgagcgccg ccgcgacgag cgccgacgag
     8101 aggagctcgc ccgccgcgca gatcgcgtcg ctcgtccgcg ccgacagctc gccgagcagc
     8161 cccacgccgg tcgcgagcga ctccagccgg gcggcttcgc gggccacctc ctcctgcagc
     8221 gccgccgtcg ccccggcccc gagtccgagg gcgtcgatcg cctcccggtg gcgcgcgacg
     8281 atctcctgga gagcggcccg cacctcctcg gcgcgtccct ctcgcgccgc ccgcgagagc
     8341 gccaggagcg tgtcggtaac gcccgccatc gccgagacga cgacgaacgg cccctcgtcg
     8401 aggcgcgagg cgacgagcga ggcggcggcc tcgatccgat ctgcgccgcc cacggacgtt
     8461 cccccgagct tcatcacgag cgccgtcggc cgtccgtggg ataccggaga ggtgcggagc
     8521 tcgggaggag gatcgtgcgc gccgtccacg gccgcctcag agccgtccgg tcgcggcgag
     8581 gagctcggcg ttcaggagcg ccgcgcccgc cgcgcctcgg acggtgttgt ggacgaggag
     8641 cgtgaaccgc acgtcgtaga ccgggtcggg ccggaggcgc cccacggtga cgaccatccc
     8701 gccccccgcg tccacgtcgc ggatcggctg cgggcggtcc cgctcgtcgc ggacgacgat
     8761 cggccgcgcc ggcgccgacg ggaggccgag gcgctgcggc tcgccgacga acgtctcgag
     8821 cgcctcgcgg acctcggcga gcgtcgtctt ccgaccgagc ttcacgaaga cggactcggt
     8881 gtgcccgtcg cggaccggta cgcggttcac cgagacggag acggggaagg aggccggctc
     8941 gacgcgtccg ccggcgagcg ccccgaggat cttccccggc tccgaggcga ccttctcctc
     9001 ctcgccgtcg atgaacggga cgacgttccc gatggcgtcg agcgacggga cgcccgggta
     9061 gccggccccc gagagcgcct ggagcgtcgt gacgcagacc gcctcgactc cgaatgcgcg
     9121 gtgcagcggc gcgagggcca ttgcgaggcc gacgacgacg cagttggggt tcgtgacgat
     9181 gccgcctccc gccttcgccc actcctgccg gtccaggagg gcgaggtggt cggcgttcaa
     9241 ctccggaatc aggagcggca cgtcggcctg catccggaac gacttcgtgt tcgagacgac
     9301 gag
//
LOCUS       DAOTEZ010000148         9247 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5294_length_9247_cov_4.742276, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000148 DAOTEZ010000000
VERSION     DAOTEZ010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9247
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5294_length_9247_cov_4.742276"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            275..637
                     /locus_tag="P5164_09285"
     CDS             275..637
                     /locus_tag="P5164_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44124.1"
                     /translation="MKSVFRALPIVLSALVLAAHFYRSRNLAMVAVSLTLPLLLFVRE
                     RWSARVVQAGLVLGALEWVRTLAFFAGQRMEVGRPWGRLAVILGVVAALTALSALAVR
                     VPLAAGAPAAGREPEATS"
     gene            634..1008
                     /locus_tag="P5164_09290"
     CDS             634..1008
                     /locus_tag="P5164_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020334839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HRY44125.1"
                     /translation="MTPAERRVAREWKTIQAMIRIYCRNNHGGRGALCEECEELRLYA
                     ERRLEKCPFSDEKPTCVKCPVHCYEAGMRERVRQVMRYSGPRMLLRHPILAIRHLRDE
                     KLPLSPKAQKVAERLKQQASGS"
     gene            complement(1021..2547)
                     /locus_tag="P5164_09295"
     CDS             complement(1021..2547)
                     /locus_tag="P5164_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRY44126.1"
                     /translation="MRPRRAAVLSLAVALLALAPAAPAAEVLLHAVTFPEGVRVDVPF
                     AGTGRAPAGATLEAEVRFEGTRTRIALSWKRMKPAILFGGDLTSYVLWAVAPDGAVEN
                     LGELFTREAKGGADYSTGRRSFGLLVTAEPDPGVARPSEIVVFTGEAPRPERAPSEPF
                     ALSRLSTEPKPATPSISTAEWAGGEPLELLQARAVLARAERMKEGDPDQRTVREARFA
                     LAQAEAAPRDGRSAPPLAQARQATALASEAIREVERRRAAEESARLEAEERAKEAARK
                     ARAEDEAERRRQAEAALAEIEELRRNAASDLQQARQSSAALELARAQLEEERAALRER
                     QAALEEERAALAARLEGALGKVAPTARTERGLVVSLSGASFENGRSALTPVARVTIGK
                     LSGVLLMSPGLNVRVEGHTDSGGSAAANRKLSSDRARIVADLLREQGIDGARLAFEGY
                     GSDRPVAPNTTAAGRAMNRRVEIVLIEDAAGDASGAKPAEPPPPTGSASPGGAPPPAS
                     "
     gene            complement(2551..3390)
                     /gene="kdsA"
                     /locus_tag="P5164_09300"
     CDS             complement(2551..3390)
                     /gene="kdsA"
                     /locus_tag="P5164_09300"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HRY44127.1"
                     /translation="MAVHASITPYLSAGDRCRPVFFAGPCVIESRAHTLKMAKAIQGV
                     FEAAGVADRLVFKASFDKANRSAGDSFRGPGIEKGLDVLSAVKERLDLPILTDVHEAE
                     QCELAAEVADVLQVPAFLSRQTDLLVAAARTKRAVNVKKGQFLAPDDCAQIVAKVRGA
                     GNPNVLLTERGSTFGYHNLVVDFRSLPMMRALGAPVIYDATHSLQLPGGLGKETGGAR
                     QYAPALARAAAAVGVDGYFLEVHDDPANAKSDRATQLPLADLPKFLFDLLKFDALRRE
                     TEG"
     gene            3500..5473
                     /gene="tkt"
                     /locus_tag="P5164_09305"
     CDS             3500..5473
                     /gene="tkt"
                     /locus_tag="P5164_09305"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRY44128.1"
                     /translation="MSPDALRRLAANTLRTLAMDGVQKANSGHPGMPMGMADVATVLF
                     TEFLKFSPKGPSWLDRDRFVLSAGHGSMLLYGLLHLSGYDLALDDLKSFRQWGSRTPG
                     HPEHGLTPGVEVTTGPLGQGVSTAVGLALAERMLAARFNRPGHEIVDHFTYAIASDGD
                     LMEGVSHEACALAAHLGLGRLVVLYDDNGISIDGPTSLSFSEDVPARFAAYGWHVARV
                     DGHDPDAVTAAIAAAKAEGARPSLIACRTHIGFGSPNRQDTSKAHGEPLGADEIRLTK
                     EKLGWPVDAQFLVPEEVRDLFGDAAARGAKAEADWNARLAAYRTAHPDLAKELDLFLD
                     GKLPDGWGAKLPAFPAEKPLATRQASGAVLDALVPAIPTLVGGSADLTPSNNTRAKTA
                     LDVKPAEYAGSYLRFGIREHGMGAILNGLALHGFRPFGGTFLVFADYMKGAIRLASLM
                     EQPVVYVFTHDSIGLGEDGPTHQPIEQLASLRATPGLVVYRPADANETAAGWRVALSR
                     THGPTALALTRQGLPVLPPNDGALRGGYVVADADGFRAILIGSGSELHLALAAQKLLA
                     ASGIPTRVVSMPSIELFLSQDEAWRESVLPRACRARVAVEAGATLTWGGIVGLDGAVV
                     GLDRFGASAPYQTLYEKLGLTAEAVAAAAQKLL"
     gene            complement(5594..6466)
                     /locus_tag="P5164_09310"
     CDS             complement(5594..6466)
                     /locus_tag="P5164_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009375805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY44129.1"
                     /translation="MSATTGPWVHREEKVGEIRLHWVEAGEGPLILLLHGFPEHWLAW
                     RRQLPALAAAGFRAVAPDLRGYNLSEKPKGVSSYRMEKLAGDVASLVRHLGYETTLLV
                     GHDWGGAIAWCVPALHPGLVERLAILNAPHPIVMKRKLATIAQARRSSYMLFFQLPWL
                     PERSLTRHRASFIRMMLRRDPATPAAFTPEEIEAYREAFLRPGAATAAVNYYRAAFRP
                     GTRVPGLRRSLEKVPALVLWGEGDRYLGPELLDGLDDLVPDLRLVRIPNASHWLPADA
                     ADLVTAELVSFFRS"
     gene            6515..7087
                     /locus_tag="P5164_09315"
     CDS             6515..7087
                     /locus_tag="P5164_09315"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRY44130.1"
                     /translation="MKRTVLALLLCAAASPILAQEKLPAAAPAAPASDPAMTGLKVAW
                     NRVKDITVRAAKKMPEESYGFQATPEVKTFGQFVGHLADSTYMMAGPVLGETPPAPEA
                     EKTKKSKAELVAALEDSFAYMDRAFALGDAAVAAPVKLFGMDTNKFAVVGLAIGHTWE
                     HYGNMVTYMRMKGIVPPSSEPRPAAPSPGK"
     gene            complement(7243..7659)
                     /locus_tag="P5164_09320"
     CDS             complement(7243..7659)
                     /locus_tag="P5164_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44131.1"
                     /translation="MELFPIDDAEMLFISPVIDDWSPVLARRIGCVIDLDGEVDHGVP
                     TLPNHMLYVYFPIFDEDLPDLARLDAVATLGTRLLESGQRVLSHCGMGFNRSALVAGL
                     ILVRRGMKGADAVARIRSRRPGALFNETFAAYLESL"
     gene            complement(7762..7971)
                     /locus_tag="P5164_09325"
     CDS             complement(7762..7971)
                     /locus_tag="P5164_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44132.1"
                     /translation="MSTPRTVETSLRYGDCGARWGNLAAPSGAFAVGDEFAFAGEEGR
                     RVVRRVLPFPTDPAKRLVHYEAAPD"
     gene            complement(7983..>9247)
                     /locus_tag="P5164_09330"
     CDS             complement(7983..>9247)
                     /locus_tag="P5164_09330"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRY44133.1"
                     /translation="TVGDIESLPFLEAIRQFRKDVGRGNAINVHVTLVPYIAASDELK
                     TKPTQHSVKELLSIGIRPDILLCRADRAIPEEMKKKIALFCNVDEPAVITARDVSTIY
                     ECPLAFAREGLDQIVLDYLSLPHSERDLTKWDRIVNRWKNPAGEVKIAIVGKYVALGD
                     SYKSLNEALHHGGIENNVKVRLEYVDSEDLETTGYPESLSRADAVLVPGGFGRRGIEG
                     MLRAIRYARETKVPFFGICLGMQCAVIEFARNVCGLANANSTEFDANAPHKVIYKLRD
                     LIGIEALGGTMRLGKYPCELAEGSTARRAYGEPLIYERHRHRYEVNQEFLPALREAGL
                     AVTGVSPDRKFVEMVELPDHPWFLGCQFHPEFKSKPTAAHPLFKDFVRAARAHQAART
                     EATAHQAAALEPGIAVPAHLPAPNGRTA"
BASE COUNT         1297 a         3279 c         3376 g         1295 t
ORIGIN      
        1 gcccgaatgc acgagacgtc gcgccacgaa aaaccgaaac gcgacccgga atcccgaatc
       61 gaccttggcc ttccgatcgc cgcgtccggg agcacggggc tctcgagaag cgggttgcgg
      121 ggtcggcccc accgttgtct accggaactg gcgaaagata cagcaagtgg cctgccatcg
      181 ggcgcggctt cggaccctgc cccttcgccc tgccgggcgc ggcatgatcc gcgtcatggc
      241 gggacatcgt gagacgtggt ctcctcgaag ggcgatgaag agcgtcttcc gagcccttcc
      301 gatcgtcctc tcggccctcg tcctcgccgc ccatttctac cgatcccgga acctcgccat
      361 ggtcgccgtc tccctgaccc tcccgctcct cctcttcgtc agggagcgat ggagcgcccg
      421 ggtcgtgcag gcggggctcg tcctcggcgc cctcgagtgg gtgcggaccc tcgccttctt
      481 cgcggggcag aggatggagg tcgggcgccc ctggggtcgc ctcgcggtga tcctcggggt
      541 cgtggccgcg ctgacggcgc tctcggcgct cgcggtgagg gtcccgctcg cagccggcgc
      601 cccggcggcc gggagggagc cggaggcgac ttcgtgaccc cggccgagcg ccgggtcgcc
      661 cgggagtgga agacgatcca ggcgatgatc cggatctact gccggaacaa ccacggcggg
      721 cggggcgccc tctgcgagga gtgcgaggag ctccgcctct acgcggagag gcggctcgag
      781 aagtgcccct tctcggacga gaagccgacc tgcgtgaagt gcccggtcca ctgctacgaa
      841 gcggggatga gggagagggt ccggcaggtg atgcgctact ccggcccccg gatgcttctc
      901 cggcacccga tcctcgcgat ccggcacctc cgcgacgaaa agctcccgct gtcgccgaag
      961 gcgcagaagg tggcggagcg gctgaagcag caggcgtccg gaagctgacg ccgggcaggt
     1021 ctaggaggcc ggcggcgggg ctccgcccgg cgaagcggag ccggtcggag gcggcggctc
     1081 ggcgggcttc gcgccggacg cgtctccggc cgcgtcctcg atcaggacga tctccaccct
     1141 ccggttcatc gcccgtcccg cggcggtcgt gttcggcgcg acgggccggt ccgacccgta
     1201 gccctcgaac gcgaggcgcg cgccgtcgat cccctgctcg cgcaggaggt cggcgacgat
     1261 gcgagcgcgg tccgacgaga gcttccggtt cgcggccgcg ctgccaccgg agtcggtatg
     1321 tccttcgacc cggacgttca ggccggggct catcaggagg acccccgaga gcttcccgat
     1381 cgtgacccgc gcgaccggcg tgagcgccga ccgcccgttc tcgaaggacg ccccggagag
     1441 gctcacgacg aggccgcgct ccgtccgtgc cgtcggcgcc accttcccga gcgcgccctc
     1501 gagccgcgcg gcgagggccg cacgctcctc ctcgagagcg gcctgccgct cccgcagcgc
     1561 cgcccgctcc tcctcgagct gcgcccgtgc gagctccagg gccgcggagg actggcgtgc
     1621 ctgctgcagg tcggaggccg cgttccggcg gagctcctcg atctcggcca gcgccgcctc
     1681 cgcctggcgc cggcgctcgg cctcgtcctc ggcacgagcc ttccgggccg cctccttcgc
     1741 ccgctcctcg gcctcgagcc gggcggattc ttcggcggca cgccgtctct cgacctctcg
     1801 gatcgcctcg gaggcgagcg ccgtggcctg ccgggcttgc gcgaggggag gcgcggaacg
     1861 accgtcgcga ggggcggcct cggcctgggc gagggcgaaa cgggcctcgc ggaccgtgcg
     1921 ctggtcgggg tcgccctcct tcattcgctc cgccctggcg aggacggccc gtgcctggag
     1981 gagctcgagc ggctccccgc ccgcccactc ggccgtcgag atcgagggag tcgccggctt
     2041 cggctcggtc gagagccggg agagcgcgaa cggctcgctc ggggccctct ccggcctcgg
     2101 ggcctctccg gtgaagacga cgatctcgga ggggcgcgcg acgccggggt cgggctcggc
     2161 cgtgacgagc aggccgaagc tcctccggcc ggtcgagtag tcggcgcccc ccttcgcttc
     2221 gcgagtgaag agctcgccga ggttctcgac ggcgccgtcc ggcgcgacgg cccagaggac
     2281 gtacgaggtc aggtcgccgc caaacaggat cgccggcttc atccgcttcc aggagagggc
     2341 gatgcgggtc cgggtccctt cgaagcggac ctcggcctcg agcgtcgcgc ccgcgggggc
     2401 gcggccggtg ccggcgaagg ggacgtcgac gcgaacccct tcggggaagg tcaccgcgtg
     2461 gaggaggacc tccgccgcgg gcgcggccgg tgcgagcgca agaagcgcga cggcgaggga
     2521 gaggacggcc gcgcggcgcg ggcgcacggc tcagccctcc gtctcccggc ggagcgcgtc
     2581 gaacttcagg aggtcgaaga ggaacttcgg gaggtcggcg agcggaagct gagtcgcccg
     2641 gtcggacttc gcgttcgccg ggtcgtcgtg cacctcgagg aagtagccgt cgacgccgac
     2701 ggcggcggcg gcgcgcgcga gcgcgggagc gtactgccgg gcgccgcccg tctccttccc
     2761 gaggccgccc gggagctgga gggagtgcgt cgcgtcgtag atcaccgggg cgcccagcgc
     2821 ccgcatcatc gggagggagc ggaagtcgac gacgaggttg tggtatccga acgtcgaccc
     2881 gcgttcggtc agcaggacgt tcgggttccc ggcgccgcgc accttcgcga cgatctgcgc
     2941 gcagtcgtcg ggggcgagga actgcccctt cttcacgttc acggcgcgtt tcgtccgcgc
     3001 ggccgcgacg aggaggtcgg tctgacggct gaggaacgcc gggacctgga ggacgtcggc
     3061 gacctcggcg gcgagctcgc actgctcggc ttcgtggacg tccgtcagga tcgggaggtc
     3121 gagcctctcc ttcacggccg agaggacgtc gagccccttc tcgatcccgg ggccgcggaa
     3181 ggagtcgccc gcggagcggt tcgccttgtc gaacgacgcc ttgaagacga gccggtcggc
     3241 gacgcccgcg gcctcgaaga cgccctggat cgccttcgcc atcttcagcg tgtgggcgcg
     3301 agattcgatg acgcacggcc cggcgaagaa gacgggccgg cagcggtcgc cggcggagag
     3361 gtagggggtg atggaggcgt ggacggccat ccccccgatc gtagcccggc cccgcctcca
     3421 tgtacgatcc gccggtcgcc ctccccgcga tggccccgcc cgggggagcc gcgcgacgac
     3481 cggacgcgaa ggagtgacca tgagccctga cgccctccgc cgcctcgcgg cgaacaccct
     3541 ccggacgctc gcgatggacg gcgtccagaa ggcgaactcc gggcaccccg ggatgccgat
     3601 gggaatggcc gacgtggcga ccgtcctctt cacggagttc ctgaagttca gcccgaaggg
     3661 cccgtcctgg ctcgaccgcg accgcttcgt cctctcggcc gggcacggct cgatgctcct
     3721 ctacggtctc ctccacctct cgggctacga cctcgccctc gacgatctga agtcgttccg
     3781 ccagtggggg agccgcacgc cgggtcaccc ggagcacggc ctgacgcccg gcgtcgaggt
     3841 gacgaccggc ccgctcgggc agggggtctc cacggccgtc ggcctcgccc tcgccgagcg
     3901 gatgctcgcg gcccgcttca atcgcccggg ccacgagatc gtcgaccact tcacgtacgc
     3961 gatcgcgagc gacggcgacc tgatggaagg ggtctcgcac gaggcgtgcg cgctcgccgc
     4021 gcacctcggc ctcggcaggc tcgtcgtcct ctacgacgac aacggcatct cgatcgacgg
     4081 cccgacgtcg ctctccttct cggaagacgt cccggcccgc ttcgccgcct acggctggca
     4141 cgtggcgcgc gtcgacggcc acgaccccga cgccgtcacg gccgcgatcg cggccgccaa
     4201 ggccgagggc gcaaggccct ccctcatcgc ctgccggacg cacatcggct tcggcagccc
     4261 gaaccggcag gacacctcga aggcccacgg cgagccgctc ggggccgacg agatccgctt
     4321 gacgaaagag aagctcggct ggccggtgga cgcgcagttc ctcgtccccg aagaggtccg
     4381 cgacctcttc ggggacgcgg ccgcacgggg cgcgaaggcc gaagccgact ggaacgcgcg
     4441 cctcgcggcg taccgtacgg cgcacccgga tctcgcgaag gagctcgacc tcttcctcga
     4501 cgggaagctc cccgatggct ggggggcgaa gctcccggcc ttcccggccg agaagccgct
     4561 cgccacgcgc caggcctcgg gtgccgtcct cgacgcgctc gtcccggcga tccccaccct
     4621 cgtcggcggc tcggccgacc tgacgccgtc gaacaacacg cgcgcgaaga ccgcgctcga
     4681 cgtgaagccg gcggagtacg ccggctcgta cctccgcttc ggcatccgcg agcacgggat
     4741 gggggcgatc ctgaacggcc tcgcccttca cggcttccgc cccttcggcg ggacgttcct
     4801 cgtcttcgcc gactacatga agggggcgat ccgcctcgcc tcgctcatgg agcagccggt
     4861 cgtctacgtc ttcacgcacg actcgatcgg cctcggcgag gacggcccga cccaccagcc
     4921 gatcgagcag ctcgcctccc tccgggcgac gcccgggctc gtcgtctacc ggccggccga
     4981 cgcgaacgag acggccgccg gctggcgcgt cgccctctcg cggacgcacg gcccgacggc
     5041 gctcgcgctc acgcgccagg gcctcccggt cctcccgccg aacgacgggg cgctgagggg
     5101 cggctacgtc gtcgcggacg cggacggctt ccgcgcgatc ctgatcggct ccggctccga
     5161 gctccacctc gcccttgccg cgcagaagct cctcgcggcc agcgggattc ccacgcgggt
     5221 cgtctcgatg ccctccatcg agctcttcct ttcgcaggac gaagcctggc gcgagagcgt
     5281 cctgccgcgc gcctgccgcg cgcgcgtcgc cgtggaggcg ggtgcgacgc tcacctgggg
     5341 cgggatcgtc ggcctcgacg gggccgtggt cggcctcgat cgtttcggcg cctcggcgcc
     5401 gtatcagacg ctctacgaga agctgggcct gacggccgaa gccgtcgcgg ccgcggcgca
     5461 gaagctcctc tgaggcacgc ggcgcccgag agtcgagacg agctccgtga gcaggggggg
     5521 caggccgaca ctcaacgctc tacgggctcc gtagagtgta gatggtagac ctgaccccct
     5581 cccttgcgtc cggtcagctc ctgaagaacg agacgagctc ggcggtcacg aggtcggccg
     5641 catcggcggg gagccagtgg ctggcgttgg gaatcctcac gaggcgcagg tcggggacga
     5701 ggtcatccag gccgtcgagg agctcggggc cgaggtagcg gtctccctcg ccccagagca
     5761 cgagagccgg gaccttctcg agagagcggc ggagcccggg gacgcgcgtc cccgggcgga
     5821 acgcggcacg gtagtagttg accgccgcgg tcgcggcccc cggccgcagg aacgcctcgc
     5881 ggtacgcctc gatctcctcg ggcgtgaagg ccgccggcgt cgcggggtcg cggcggagca
     5941 tcatccggat gaagctcgcc cggtgacggg tcagcgaccg ctcggggagc caggggagct
     6001 ggaagaagag catgtacgac gaccgccgcg cctgggcgat cgtcgcgagc ttccgcttca
     6061 tcacgatcgg gtgaggggcg ttcaggatcg cgagcctctc gacgaggccg ggatggagcg
     6121 ccgggacgca ccaggcgatc gccccgcccc agtcgtggcc gacgagcagc gtcgtttcgt
     6181 atccgaggtg gcggaccagg ctcgcgacgt cgcccgcgag cttctccatc cggtagctcg
     6241 agacgccctt cggcttctcc gagaggttgt agccgcgcag gtcgggggcg acggcgcgga
     6301 agccggccgc tgcgagcgcc gggagctgcc tgcgccacgc cagccagtgc tcggggaagc
     6361 cgtgaaggag gaggatgagc ggcccctcgc ccgcctcgac ccagtgcagt cgaatctcgc
     6421 cgaccttctc ctcgcggtgg acccacgggc ccgtcgtcgc gctcaccacc cgatgctaat
     6481 ctccgccggc cgcccgacag aagaaggagg ccccatgaag aggaccgttc tcgcgttgct
     6541 cctttgcgcc gccgcttcgc ccatcctcgc ccaggagaag ctgccggcag cggctcccgc
     6601 ggcccccgcc tccgacccgg cgatgaccgg cctgaaggtc gcgtggaacc gcgtcaagga
     6661 catcaccgtc cgcgccgcga agaagatgcc ggaagagagc tacggcttcc aggcgacgcc
     6721 cgaggtgaag acgttcggac agttcgtcgg ccacctcgcc gactcgacgt acatgatggc
     6781 cggccccgtc ctcggcgaga cgccgcccgc tccggaggcc gagaagacga agaagtcgaa
     6841 ggccgagctc gtcgcggcgc tcgaggactc cttcgcgtac atggaccgcg cgttcgccct
     6901 cggcgacgcc gccgtggccg cccccgtgaa gctcttcggc atggacacga acaagttcgc
     6961 cgtcgtcggc ctcgccatcg gccacacgtg ggagcactac gggaacatgg tcacctacat
     7021 gcggatgaag gggatcgtcc cgccgtcgag cgagccgcgc cccgcggctc cttcccccgg
     7081 gaagtagcag ggacgaacgg cccgccgatg ggcctccgcc ccgacttcac ggaacgaggg
     7141 cggaaggagg gggtcacgga aggagggggt caggtctacc atctacactc tgcggggccc
     7201 cgcagagtgt agatggtaga cctgaccccc cccgcctcgt cttcagaggc tttcgaggta
     7261 ggcggcgaag gtctcgttga agagggcgcc ggggcgacgg ctgcggatgc gggcgacggc
     7321 gtcggcgccc ttcatcccgc gccgcacgag gatcagcccg gcgacgagcg cggaacggtt
     7381 gaagcccatg ccgcagtgcg agagcactct ctgcccgctc tcgaggagcc gcgtcccgag
     7441 cgtggcgacg gcgtcgagcc tcgccaggtc ggggaggtcc tcgtcgaaga tcgggaagta
     7501 gacgtagagc atgtggttcg gaagggtcgg gacgccgtgg tccacctcgc cgtcgaggtc
     7561 gatcacgcag ccgatcctcc gggcgaggac cgggctccag tcgtcgatga ccggagagat
     7621 gaacagcatc tcggcgtcgt cgatcgggaa gagctccatg gtcgggagca ctctaccgcg
     7681 acctccggcc ggaggcatcc ggcgcggacg tgcgggcgcg cattgcgacg cggcacgcgg
     7741 cgccggcgta cgaggcggtc ctcagtccgg cgcggcctcg taatggacga gccgcttcgc
     7801 ggggtcggtc gggaagggga ggacgcggcg gacgacgcgg cgcccctcct ccccggcgaa
     7861 ggcgaactcg tctccgaccg cgaacgcgcc ggagggcgcg gccaggttcc cccagcgcgc
     7921 cccgcagtcg ccgtagcgga gggaagtctc gaccgtgcgc ggagtcgaca tcggctccgc
     7981 cgtcacgccg tccggccgtt cggggcgggg aggtgggccg ggacggcgat gccgggctcg
     8041 agcgcggcgg cctggtgcgc ggtcgcctcg gttcgggccg cctgatgggc gcgcgccgcc
     8101 ctcacgaagt ccttgaagag gggatgggcg gccgtcggct tcgacttgaa ctcggggtgg
     8161 aactggcagc cgaggaacca ggggtggtcc ggcagctcga ccatctcgac gaacttccgg
     8221 tcgggcgaga cgccggtgac ggcgagcccc gcctcgcgga gcgccgggag gaactcctgg
     8281 ttcacctcgt agcggtggcg gtggcgctcg tagatgagcg gctcgccgta ggcgcgccgc
     8341 gccgtcgacc cctcggcgag ctcgcacggg tacttgccga gccgcatcgt gccgccgagc
     8401 gcctcgatcc cgatcaggtc gcggagcttg tagatgacct tgtgcggggc gttcgcgtcg
     8461 aactcggtgg agttcgcgtt cgcgaggccg cagacgttcc gcgcgaactc gatgacggcg
     8521 cactgcatgc cgaggcagat gccgaagaac ggcactttcg tctcgcgggc gtagcggatg
     8581 gcgcggagca tcccctcgat gccgcggcgc ccgaagccgc ccgggacgag gacggcgtcg
     8641 gcgcgcgaga gcgactcggg gtagccggtc gtctcgaggt cctcggagtc gacgtactcg
     8701 aggcgcacct tgacgttgtt ctcgatgccg ccgtggtgga gcgcctcgtt cagcgacttg
     8761 tacgagtcgc cgagcgcgac gtacttgccg acgatcgcga tcttcacctc gcccgccggg
     8821 ttcttccagc ggttgacgat ccggtcccac ttcgtcaggt cgcgctcgga gtgggggagc
     8881 gagaggtagt cgaggacgat ctggtcgagc ccttcccgcg cgaaggcgag cgggcactcg
     8941 tagatcgtcg agacgtcgcg cgccgtgatg acggccggct cgtcgacgtt gcagaagagc
     9001 gcgatcttct tcttcatctc ctccgggatc gcgcggtcgg cgcggcagag gaggatgtcg
     9061 gggcggatgc cgatggagag gagctccttg acggagtgct gcgtcggctt cgtcttcagc
     9121 tcgtcggagg cggcgatgta ggggacgagc gtcacgtgga cgttgatcgc gttcccgcgg
     9181 ccgacgtcct ttcggaactg gcggatcgcc tcgaggaagg ggagcgactc gatgtcgccg
     9241 acggtgc
//
LOCUS       DAOTEZ010000149         9224 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5320_length_9224_cov_6.279202, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000149 DAOTEZ010000000
VERSION     DAOTEZ010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9224
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5320_length_9224_cov_6.279202"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1838
                     /locus_tag="P5164_09335"
     CDS             <1..1838
                     /locus_tag="P5164_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRY44134.1"
                     /translation="LILAAQFESFIDPLSIMLSLPLSIVGMAGMLALTRDTLSIMSLI
                     GLILLMGLVTKNAILLVDYTKVLRKQGLERREALITAGRTRLRPILMTTLAMIFGMLP
                     LALGLGQGAEMRAPLGRAVIGGLITSTILTLLVVPVVYALFDDFALFLHRRWTKANAL
                     EQDAHGHTGKAAAKAAGAALLLLSLLVPAPAGAAGATGASESAAAPTVLTLDDALRIA
                     QEKNRDVAKARAYQSWVRGKYVEERAGALPTVAATGSLGRSWDGTYAALTDGLFPSGQ
                     TVKTAGVSLTQTLFAWGKVGAAVRAAKDGIASAEDQLEHYRQAAVRDVTEAFHDVLLA
                     RRLEAIARETLEHRERQLEEAEKRHVLGTATDYDVLAARVARDNQRPEVLRTASGVLV
                     ALDRMRLVLAEESREIDVEGTLEAEVAELPSFEEAVSTALEKRPDLSGLVRNAAIYRQ
                     LVRIRNADDKPRLDLRGSAGWQWFDAGVVSASGKVWGAGLYLSFPFFDGLATRGRVAQ
                     ARSDLTRAELDLAQARDGIHVEVRTALDRARVAAEIVKALSGNVAQARRLLEMSETGR
                     ELGVKTRLEVDDALLNARAAEANLARAKRDYLVALADLRYAQGTL"
     gene            1870..2385
                     /locus_tag="P5164_09340"
     CDS             1870..2385
                     /locus_tag="P5164_09340"
                     /inference="COORDINATES: protein motif:HMM:NF020904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HRY44135.1"
                     /translation="MSTGRYRHLLVDAPKGPPTDAEIAAIEEAVGAPLPEELREWLAA
                     FNGGTLDYTFELDTEAGPFPLCFYSLYSTRRPSPARPAAGLILHELAMERRLKELRPG
                     LLPIAGDGGTSVLYVDLAPERRGSVVAYVEGLPAWDGTPEGCFVGVAPDLPAYAAGLY
                     LNTVEEPRRGG"
     gene            2472..2951
                     /locus_tag="P5164_09345"
     CDS             2472..2951
                     /locus_tag="P5164_09345"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRY44136.1"
                     /translation="MKTIGATVLAFCLGGASVLGATPEARGGGTRVAASADVASRARM
                     LLETQAAAWNRGDLEAFCSVYAEDALFLTAFGLTKGRAEVLARYRARYPDARARGTLS
                     FEVLSVDRLAGGAEGEAIAVVARWTISHPDRPAATGLTLLNLLRSGAGWKIVHDASM"
     gene            3020..3544
                     /locus_tag="P5164_09350"
     CDS             3020..3544
                     /locus_tag="P5164_09350"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HRY44137.1"
                     /translation="MPQRRTSALVAAAAVLATSVTLAGGSPPRPAPRAAIAASLADIA
                     WLSGGWVVEKGETYSEEWWSAPSGDSMVGAWRLSISGRARLFELLTLMEEEGRVVLRI
                     RHFDAKGVAWEEKDRPLVLPLVEKGTRQAVFEGTEGEGLLRISYLGDAESLTVEIAKT
                     GKPVQSFRFLRAVR"
     gene            complement(3590..5158)
                     /locus_tag="P5164_09355"
     CDS             complement(3590..5158)
                     /locus_tag="P5164_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44138.1"
                     /translation="MPRHSSSLALAVLLLVSPSAGAAPPPNDTCPAAITISGLELPYT
                     QMLETVLATSDGDPAMPCATSTPGKSVWFKFQPATSDSYVFETGGSTPADYQPILALY
                     TGGCAGLTPVAGACGRGRLVSFLEAGATYYLLVAGDAIVVNPAIRIALDGVDLCPGGP
                     GPGGGGCGGAISVRVGDALSARAYNELNDTLLLTGAFSWDLGQNASPAGSTSPSVAFQ
                     YTAPVASTNVILNWTPPGQSPITRQLPISVGAAGNLVATSDEPGAPVLFAAGETATLP
                     SPGGILRLSVQRNAPDWRYNSLIPSVASTKNAAGVPFVSDLSLTNLESTETIVGLELW
                     TTAGRREASSIRLAPKGSKTVPDVVKTAFGLEQTFGTLYISSTGAIVAGARTWAPFAG
                     GTTGQFALAGDVRTSSSPALLRTGEVGVLSGARQDSAFRTNIGVYNVSDKECVVELEA
                     KDDEGHTVGSKLALTLPPTRYAQEPLTRLTTSLPSGSVTLTNGTSGCYVGGVAYVIDN
                     VTQDPFAVSQRKKP"
     gene            5250..7451
                     /locus_tag="P5164_09360"
     CDS             5250..7451
                     /locus_tag="P5164_09360"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRY44139.1"
                     /translation="MSRTAWGAIALAGVLCASPAAAQPERKPTDRPTVGLVLSGGSAH
                     GLAHVGVLKVLHELRIPVDVVTGTSMGSVVGGLFAAGMSPPEMEWLLGTIDWEDILDD
                     RPSRDRLSFRRKQDEVPSYVDLEMGLTPRGIAFPSGLIAGQKLGFLLKSLTLGTVGIG
                     KFDDLPIPFRCVATDIATGEKVVLSDGSLAEAIRASMSIPGVFSPVPRGARLLVDGGL
                     VENLPVGEARDLGADVVIAVDVSSDKFDPERLKSFGGVLSRMTSLPVVRNVSEAAPRA
                     NVVLSPRVGDIPSMGFGRAAEIVARGEEEARRLAHLLSALSMTEEEYRAHRERVRGRR
                     ADRIVVDEIQTISVGVDPRQVRARLETEPGRPLDIGALRRDLDRIYEMGVFETVEFQI
                     VRDEGRHVLVIDARPKSWGPTFVRVGSGFEADFDGDSTVALRGTLHAMQLNSRGGEVK
                     ATLELGTVPGLRVEYYQPLDFRGRTFFSVDASFVRTLVRVSAPDGFVGEGRITLLQGS
                     VDAGLSLGSWGQVRAGVFRGTGRGEPLLTNEPIDDLEIDVGGLFAQASVDTLDDLSFP
                     RRGTLAVAAVEAFRPGLGSETSFNRLSLRGTRAFSVGRRTALHVTGGWEDTMGSHPPF
                     YLLFSLSDFNRLAALSREAATGERAAYAAVAVQQRVASLPTRVGRGVYLGGLFEAGRV
                     WARREDTGFDGIRPAGGVFVGADTVLGPIYAGFGVGYGGNTTLYVFLGRPY"
     gene            complement(7518..8306)
                     /locus_tag="P5164_09365"
     CDS             complement(7518..8306)
                     /locus_tag="P5164_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44140.1"
                     /translation="MKSVTAAAALAALVAATPLARASEPTVVWYPSVVNTEGLNGARY
                     YSVLSVWNPSPTEITLTVDGFVSNATGLAAPSATVTATVPGATHATIPNVLASLFGGL
                     EGLAAARITASGPATFFHSVVNDQTAAGNGRTLLKVDNARGANVLSGGTLLQLSNASA
                     EDRARGLGKRTNVGYFNPSFDTKSVRFRAWTSNATLIGETIRSIPAWSQVQQPVFDLV
                     PPSTAGNSVHEDFLVTFEVQGGGLLVYSTVVDNKTNDGVYQAAE"
     gene            8567..>9224
                     /locus_tag="P5164_09370"
     CDS             8567..>9224
                     /locus_tag="P5164_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44141.1"
                     /translation="MNVRPTRFFALIAAVLPLAAASSSAATVPLPNGGFELNAASGAA
                     SWSVDGTLPPGADVVRAADVRHAGDASLRLSAADPASITVSSEEVRLEVGHVYRLSAF
                     VRTEEAVSDPTGRYPTPVAATVTMASFPFTNHSPSAGATRPFGKVETLFVATTAKDRV
                     RLHLGRNGTATGTAWFDDVALEEVTDVRELVPMEAVRWHGPAFRYDEKGWIYVHVEGE
                     P"
BASE COUNT         1218 a         3190 c         3453 g         1363 t
ORIGIN      
        1 acctcatcct cgcggcgcag ttcgagtcgt tcatcgaccc gctctcgatc atgctgtccc
       61 ttccgctctc gatcgtcggc atggcgggga tgctcgccct gacgcgcgac acgctgagca
      121 tcatgtcgct catcggcctc atcctcctga tgggcctcgt gacgaagaac gcgatcctcc
      181 tcgtcgacta cacgaaggtc ctccggaagc aggggctgga gcgtcgcgag gcgctcatca
      241 cggcggggcg gacgcggctc cggccgatcc tcatgacgac cctcgcgatg atcttcggca
      301 tgctcccgct cgccctcggc ctcggccagg gggcggaaat gagggcgccg ctcgggcgcg
      361 ccgtcatcgg cggcctgatc acgtcgacga tcctcacgct cctcgtcgtc ccggtcgtct
      421 acgccctctt cgacgacttc gctctcttcc tgcaccgccg gtggacgaaa gcgaacgccc
      481 tcgagcagga cgcgcacggt cacacgggca aggcagccgc gaaggccgcc ggggcggccc
      541 tccttcttct ctccctcctc gtcccggcgc cggccggcgc cgcgggagcc accggcgctt
      601 ccgagtccgc cgcggccccc acggtcctga cgctcgacga cgccctccgg atcgcccagg
      661 agaagaaccg cgacgtcgcg aaagcgcgcg cctaccagag ctgggtccgc ggcaagtacg
      721 tcgaggagcg cgccggggcg cttccgacgg tggcggcgac gggatctctc ggccgttcgt
      781 gggacggcac ctatgccgct ctgacggacg ggctcttccc ctcggggcag accgtgaaga
      841 cggcgggcgt ctccctcacg cagacgctct tcgcgtgggg gaaggtcggc gccgccgtcc
      901 gggcggcgaa ggacggcatc gcctcggccg aggaccagct cgagcactac cggcaggccg
      961 cggtccgcga cgtgaccgag gcgttccacg acgtcctcct cgcgaggcgc ctcgaggcga
     1021 tcgcgcggga gaccctcgag caccgcgagc ggcagctcga ggaggcggag aagcgccacg
     1081 tgctcgggac cgccacggac tacgacgtcc tcgcggcccg ggtggctcgc gacaaccagc
     1141 gccccgaggt gcttcgtacg gccagcggag tcctcgtggc gctcgaccgc atgaggctcg
     1201 tcctcgccga ggagtcgagg gagatcgacg tcgaagggac gctggaggcc gaggtcgcgg
     1261 agctcccgtc cttcgaggag gcggtgtcga cggccctcga gaagcgcccg gacctctccg
     1321 gtctcgtccg gaacgcggcg atctaccgcc agctcgtccg gatccggaac gccgacgaca
     1381 agccccgcct cgacctccgc gggagcgccg gctggcagtg gttcgacgcg ggcgtcgtct
     1441 cggcgtccgg caaggtctgg ggagcggggc tttacctttc cttcccgttc ttcgacggac
     1501 tggcgacgcg gggacgcgtc gcgcaggcgc ggagcgacct gacccgcgcc gagctcgacc
     1561 tcgcccaggc gcgcgacggg atccacgtcg aggtccggac ggcgctcgac cgcgcgcggg
     1621 tcgcggcgga gatcgtgaag gccctctcgg ggaacgtcgc gcaggcgcgg cggctcctcg
     1681 agatgtcgga gacggggcgc gagctcggcg tgaagacgcg cctcgaggtg gacgacgccc
     1741 tcctgaacgc ccgggcggcg gaggcgaacc tcgcccgcgc gaagagggac tacctcgtcg
     1801 cgctcgccga cctccggtac gcgcagggga cgctctgacg cggcgcttcg cgtcgggcac
     1861 cagggccgga tgagcaccgg acgctacagg cacctgctcg tcgacgcgcc gaaggggccg
     1921 ccgacggacg ccgagatcgc cgcgatcgag gaggccgtcg gcgcccctct tccggaggag
     1981 ctccgtgagt ggctggccgc cttcaacggc gggacgctcg actacacctt cgagctcgac
     2041 accgaggccg gcccgttccc gctctgcttc tactccctct actcgacccg ccggccgtcg
     2101 ccggcccggc ccgccgccgg cctgatcctc cacgagctcg cgatggagcg gcgcctgaag
     2161 gagctccggc cgggcctcct tccgatcgcc ggggacggcg gcacgtcggt cctctacgtc
     2221 gacctcgcgc cggagcggcg gggttccgtc gtggcgtacg tcgaggggct cccggcgtgg
     2281 gacgggacgc cggaagggtg tttcgtgggg gtggcgcccg atctccccgc gtacgccgcc
     2341 ggcctctacc tgaacaccgt ggaggagccg aggcgcggag ggtaggggcg gcgcgacggc
     2401 ggtgctcctt ccgccggtcg aacctcgcgg cgggcgcggc tttcgcgggg cgagacggtt
     2461 aggattcggg catgaagacg atcggtgcga ctgtcctggc cttctgtctg ggcggagcgt
     2521 ccgtgctcgg ggcgactccg gaagcgcggg gaggcggaac gcgggtcgcc gcctcggcgg
     2581 acgtcgcctc ccgcgcccgg atgctcctcg agacgcaggc ggccgcctgg aaccggggag
     2641 acctcgaggc cttctgctcc gtctacgccg aggacgccct cttcctgacc gcgttcggcc
     2701 tcacgaaggg gcgggcggag gtgctggcgc gctaccgggc tcgctatccc gatgcgcgcg
     2761 cgcgcgggac gctctcgttc gaggtcctct cggtcgaccg gctcgcgggc ggggccgagg
     2821 gcgaggcgat cgcggtcgtc gcccgctgga cgatctctca cccggaccgt cccgcggcga
     2881 cgggcctcac gctcctcaac ctcctgcgga gcggggcggg gtggaagatc gtccacgacg
     2941 cctcgatgtg agcgagccgc cgctccttcg cgctgcgagc gcgaacgcat tccgcccgac
     3001 ctcgcagagg agatgaccga tgccccagag acgaacgtct gctctcgtcg ccgccgcggc
     3061 cgtcctcgcg acctccgtca ctctcgccgg gggatcgccg cctcggcccg cgccccgcgc
     3121 ggcgatcgcc gcctccctcg cggacatcgc ctggctctcc ggcggctggg tcgtcgagaa
     3181 gggcgagacg tactcggagg agtggtggtc ggcgccctcg ggcgactcga tggtcggagc
     3241 gtggcgcctg tcgatctcgg ggcgcgcgag gctgttcgag ctcctgacgc tgatggaaga
     3301 ggaggggcgg gtcgtcctcc ggatccgtca cttcgacgcg aagggcgtcg cgtgggagga
     3361 gaaagatcgc cccctcgtcc tgccgctcgt cgagaaggga acgcggcagg ccgttttcga
     3421 agggacggag ggggaggggc tgcttcgcat ctcctacctc ggggacgcgg agtcgctgac
     3481 ggtcgagatc gcgaagacgg ggaaaccggt gcagagcttc cggttcctcc gggcggtgag
     3541 atgagccccg ggggcgcgcc cgaggcgcgc ccccggaagc tcgagctcgt tacggcttct
     3601 ttcgctgcga gaccgcgaag gggtcctggg tgacgttgtc gatgacgtag gcgacgccgc
     3661 cgacgtagca gccggaggtc ccgttcgtga gggtaacgga gcccgagggg aggctcgtcg
     3721 tgaggcgcgt gagcggctcc tgcgcgtagc gggtcggcgg gagcgtaagg gcgagcttcg
     3781 agccgacggt gtgcccctcg tcgtccttcg cctcgagctc gacgacgcat tccttgtcgc
     3841 tcacgttgta gacgccgatg ttcgtccgga acgcggagtc ctgccgggcc ccggagagga
     3901 ccccgacctc gcccgtccgc aggagggcgg gggaggagct cgtgcggacg tcgccggcga
     3961 gggcgaactg accggtcgtc cccccggcga agggcgccca cgtccgcgcc ccggcgacga
     4021 tcgcgccggt cgaggagatg tagagcgtgc cgaaggtctg ctcgaggccg aacgccgtct
     4081 tcacgacgtc gggcaccgtc ttcgagccct tcggcgcgag gcggatcgag gaagcttccc
     4141 gccggcccgc cgtcgtccag agctcgaggc cgacgatcgt ctcggtcgac tcgaggttcg
     4201 tgagggagag gtcggagacg aaggggacgc cggcggcgtt cttcgtgctc gcgaccgagg
     4261 ggatcaggct gttgtaccgc cagtcgggcg cgttcctctg gacggagagg cgaaggatcc
     4321 ctcccggtga agggagggtc gccgtctcgc ccgccgcgaa gaggacgggg gccccgggct
     4381 cgtccgaggt cgccacgagg ttcccggccg cgccgacgga gatggggagc tggcgggtga
     4441 tcgggctctg ccccggaggc gtccagttca ggatgacgtt cgtcgacgcg accggagccg
     4501 tgtactggaa ggcgaccgac gggctcgtcg agccggcggg ggaggcgttc tggccgaggt
     4561 cccagctgaa ggctccggtg aggaggaggg tgtcgttcag ctcgttgtag gcgcgggccg
     4621 agagcgcgtc tccgacgcgg acggagatcg cgccgccgca gccgccgccg cccggcccgg
     4681 gcccgcccgg gcagaggtcg acgccgtcga gggcgatccg aatggcgggg ttcacgacga
     4741 tggcgtcgcc ggcgaccagg aggtagtacg tcgcgccggc ctcgaggaag gagacgagcc
     4801 ggccgcggcc gcacgctccc gcgacggggg tgagcccggc gcagccgccc gtgtacaggg
     4861 cgaggatcgg ctggtagtcg gcgggcgtgc tcccgccggt ctcgaagacg taggagtcgg
     4921 aggtggccgg ctggaacttg aaccagaccg acttccccgg ggtcgaggtc gcgcacggca
     4981 tcgcgggatc gccgtccgag gtggccagga cggtctcgag catctgggtg tacgggagct
     5041 cgagcccgga gatcgtgatg gcggcggggc aggtgtcgtt cggcggcggg gcggcgccgg
     5101 cggacggcga gacgagaagc agaaccgcga gggcgaggga cgaggagtgt ctgggcatcc
     5161 ggggtcctcc tgcaggggtg gattgaaggc gaaggttcga cggtagtgtc gctcggtcgg
     5221 cggttcccac gcacggctat gatgcgccga tgtcccggac cgcttgggga gcgatcgctt
     5281 tggcgggtgt cctctgcgcg tcgcctgccg ccgcgcagcc ggagaggaag ccgacggaca
     5341 ggccgacggt cggtcttgtc ctgtcgggag gctcggccca cgggctcgcc cacgtcggcg
     5401 tcctgaaggt ccttcacgag ctgcggatcc cggtcgacgt cgtcacgggg acgagcatgg
     5461 gctcggtcgt cggagggctc ttcgccgccg ggatgtcgcc gcccgagatg gagtggctcc
     5521 tcggcacgat cgactgggag gacatcctcg acgaccgccc gtcgcgtgac cgcctctcgt
     5581 ttcgtcggaa gcaggacgag gtgccgagct acgtcgacct cgaaatgggc ctgaccccgc
     5641 gggggatcgc gttcccctcc gggctcatcg ccgggcagaa gctgggcttc ctcctcaagt
     5701 ctctgacgct cgggacggtg ggcatcggaa agttcgacga cctgccgatc ccgttccgat
     5761 gcgtcgcgac ggacatcgcg acgggagaga aggtcgtcct ctccgacggg agcctcgccg
     5821 aggcgatccg ggcgagcatg tcgatcccgg gcgtcttctc gccggtcccg cggggcgcgc
     5881 gtctcctcgt cgacggcggg ctcgtggaga acctcccggt cggcgaggcg cgcgacctgg
     5941 gggccgacgt cgtcatcgcc gtcgacgtct cgagcgacaa gttcgacccg gagaggctga
     6001 agtcgttcgg gggcgtcctc tcccggatga cgagcctgcc ggtcgtgcgg aacgtctcgg
     6061 aggccgcccc gcgcgcgaac gtcgtcctct ccccccgcgt cggggacatc ccctcgatgg
     6121 gcttcggccg cgcggcggag atcgtcgcgc gcggggagga ggaggcccgc cggctcgcgc
     6181 acctcctctc ggcgctctcc atgacggaag aggagtaccg ggcccaccgg gagcgggtcc
     6241 gggggcggag ggccgaccgg atcgtcgtcg acgagatcca gaccatctcc gtcggcgtcg
     6301 acccgaggca ggtccgggct cgcctggaga ccgagccggg gcggccgctc gacatcgggg
     6361 cgctccgccg cgacctcgac cggatctacg agatgggcgt cttcgagacg gtggagttcc
     6421 agatcgtccg cgacgagggg cggcacgtcc tcgtcatcga cgcgcggccc aagtcgtggg
     6481 gtccgacgtt cgtccgggtc ggaagcggct tcgaagcgga tttcgacggc gactcgacgg
     6541 tcgccctgcg cgggacgctc cacgcaatgc agctgaactc ccggggcggc gaggtgaagg
     6601 cgacgctcga gctggggacg gtccccggcc tgagggtcga gtactaccag ccgctcgatt
     6661 tccggggccg gaccttcttc agcgtcgacg cctccttcgt ccggaccctc gtgcgcgtct
     6721 ccgccccgga cggcttcgtc ggcgaggggc ggatcacgct cctccagggc tcggtcgacg
     6781 ccggcctttc tctcggctcg tggggccagg tgcgcgccgg ggtctttcgc ggaacggggc
     6841 gcggcgagcc tctcctgacg aacgagccga tcgacgacct cgagatcgac gtcggcggcc
     6901 tcttcgcgca ggcctccgtc gacacgctcg acgacctctc gttcccccgg cgcgggacgc
     6961 tggccgtggc cgcggtggag gcgttccgcc ccgggctggg ctcggagacc tccttcaacc
     7021 ggctgtcgct gagggggaca cgggccttct ccgtcggaag gaggacggcg cttcacgtca
     7081 cgggcgggtg ggaggacacg atggggagcc acccgccgtt ctacctcctc ttctcgctgt
     7141 ccgacttcaa tcgcctcgcg gccctctctc gggaagcggc gaccggcgag cgcgccgcct
     7201 acgcggccgt cgccgtccag cagagggtcg cgagcctccc gacgagggtc gggcgcggcg
     7261 tctatctcgg tggccttttc gaagccggcc gcgtctgggc gcgacgcgag gacacggggt
     7321 tcgacgggat ccgcccggcc gggggcgtct tcgtcggcgc cgacacggtc ctggggccga
     7381 tctacgccgg cttcggcgtc ggctacggcg gcaacacgac gctctacgtc ttcctcgggc
     7441 gtccctactg acggaaagag ccggggcatt cgccccggct cgactccgcg agtcccgggc
     7501 tcaggcccgg ggccgcttca ctcggcggcc tggtagacgc cgtcgttcgt cttgttgtcg
     7561 acgacggtcg agtagaccag gagcccgccc ccctgcacct cgaacgtcac gaggaagtcc
     7621 tcgtggaccg agttgccggc cgtggacggc gggacgaggt cgaagaccgg ctgctgcacc
     7681 tggctccagg ccgggatcga tcggatcgtt tctccgatca gggtcgcgtt cgaggtccag
     7741 gcgcggaatc gcacgctctt ggtgtcgaag ctcgggttga agtagccgac gttcgtcctc
     7801 tttcccagcc ccctcgcacg gtcctcggcg ctcgcgttcg agagctgcag gagcgtgccg
     7861 cccgaaagga cgttcgcgcc ccgggcgttg tcgaccttca ggagcgtccg gccgttcccc
     7921 gccgccgtct ggtcgttgac gacggagtgg aagaacgtcg ccgggccgct cgcagtgatg
     7981 cgtgcggccg cgaggccctc gagcccgccg aagagggagg ccaggacgtt cgggatggtg
     8041 gcgtgcgtcg cccccgggac ggtggcggtc acggtcgcgg acggggccgc gagccccgtg
     8101 gcgttcgaga cgaagccgtc cacggtgagg gtgatctcgg tcggggacgg gttccagacg
     8161 ctcaggaccg agtagtaccg ggcgccgttc aggccttccg tgttcacgac ggacggatac
     8221 cagacgaccg tcggctcgga ggcgcgggcg agcggagtgg cggcgacgag ggcggcaagg
     8281 gccgcggcgg cggtgacgct cttcatgttc atactcctgt cggcggcgtt ttccggggac
     8341 gcgtcgatta cgcccccgga tcgcggcgcg gatcccgggg gaagaccgcg gtgtgaccca
     8401 tctcacacgc ggggaccgtc ggcgtctccg cagcggcggg atgccgccga cgcccgcctg
     8461 acggagcgtt ccgatcggtc gctcggcggg atccgcggtc ccgagtcgtg ggccggcgcc
     8521 cgggacgtta cgattcgcgg attcgggctc catcgaagga gggaccgtga acgttcgacc
     8581 gacgcgcttc ttcgccctga tcgccgccgt cctgcccctc gcggccgcgt cctcgtccgc
     8641 ggcgaccgtg ccgcttccga acggcggctt cgagctgaat gccgcttcgg gcgccgcctc
     8701 gtggagcgtc gacggcacgc tcccgccggg ggcggacgtc gtgcgcgccg cggacgtgag
     8761 gcacgccggc gacgcgagcc tgcgtctctc ggcggcggac cccgcgtcga tcaccgtctc
     8821 ctccgaggag gtccgactcg aggtgggcca cgtctaccgc ctctcggcgt tcgtccggac
     8881 ggaggaggcc gtctccgacc cgacggggcg ctacccgacg cccgtcgccg cgaccgtcac
     8941 gatggcctcc ttccccttta ccaaccactc gccctcggcc ggggcgacgc gcccgttcgg
     9001 gaaggtcgag acgctcttcg tcgcgacgac ggcgaaggac cgcgtccgcc tccacctggg
     9061 ccggaacggg acggcgaccg ggaccgcctg gttcgacgac gtggcgctcg aggaggtgac
     9121 ggacgtccgc gagctcgtcc cgatggaggc ggtccgctgg cacgggcccg ccttccgcta
     9181 cgacgagaag ggatggatct acgtccacgt cgagggggag ccgt
//
LOCUS       DAOTEZ010000150         9202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5339_length_9202_cov_6.845086, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000150 DAOTEZ010000000
VERSION     DAOTEZ010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9202
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5339_length_9202_cov_6.845086"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /locus_tag="P5164_09375"
     CDS             <1..1372
                     /locus_tag="P5164_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRY44142.1"
                     /translation="FEPLTFEDVMAVIEKEKPAGVLVQFGGQTPLKLAHPLKEAGVPI
                     LGTSPDAIDRAEDRERFGELLRETGLAAPPWGTARDVAEGLAAGAKLGFPLLVRPSYV
                     LGGRAMRIVYDDAALRGVLEEALEAFPGRPVLLDRFLEDAFELDVDAVGDGTDVVVAG
                     VMQHIEEAGIHSGDSYAVIPPYRRVPRESLGEIREATRRLGQALGVVGLMNVQFALRE
                     GKLFVLEVNPRASRTVPFVAKATGVPLARIAARLCLGQTLEEVGLTKEPPVPGVSIKA
                     PVFPFRRFEGVDTILGPEMRSTGEVMGRDRSFGLAFLKAAAGAGVRLPETGTVFCSVH
                     DGDKEALLPIARELSALGFRLSATGGTAAFLHAAGLEATPVLKVNEGRPNVVDRMING
                     EIQLVVNTPLGKESFYDEVAIRRNALERDIPCLTTLTAAAAALEAIRARAGGPPSVTP
                     LQDAGA"
     gene            1372..3348
                     /locus_tag="P5164_09380"
     CDS             1372..3348
                     /locus_tag="P5164_09380"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRY44143.1"
                     /translation="MDGRRGRLLPLAAFAARASLALALLALAFAALRIPWQPAPIPLL
                     AFAALVLGASLARPNVAVLLSAAALPLLPILGNLFGGPWVAPAELVLLLLAAAVLLRG
                     GSPALSAPLPRTLAISTAAMAAGAFAATLAAVAPAELPAAVRELSSSFFSAGSDHAAA
                     PLRAALVHACGLLGFALFRRVAAARGEGTPLAALVAGLALVGAFAVVEAATGLKLWSP
                     AHYEALSAGLRVPSTLPDYNATGAAMALGLFPALALAYAARGARRLAWALAAGLLLAG
                     LLLSGSRTAWLAALLAGGATLLGALHARRKEARGGSRRLLLGASLAAGLAVAAVAAWP
                     GDVGALLRRRAATLLEPEAALKAVRAGRLGFWTAGARMVAEHPAAGVGPGRVPARFDD
                     FCDPGFPVRNENLHNYFLQAVAENGVPGGLLVLLPFVPLGLLLGRSLASGAAFVSPAA
                     ALAPGLLAFTATGVASHPWLLPELQLLFWSAAALLPGAARGPAPEPRFRRAGVLALAT
                     LAAWGGFVLLARPGQERGRWGAGEWSAEGAEKPYFWVGPRALVPVEVPGGERLSVRLR
                     GMQEGLGRRPLVVALRLDGGPVRKVSVPTRAWQDVVVERGPLRPGIAPARGGLLAVEA
                     SRGFLPSGDGRGDRRVLALQLARPPLRPAPEAMP"
     gene            3345..5330
                     /locus_tag="P5164_09385"
     CDS             3345..5330
                     /locus_tag="P5164_09385"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRY44144.1"
                     /translation="MNRPSPVAGILAFLGWVVLAALCSVVLLPPLPSLRTAVWGASVV
                     LFAAAVLAPLRSLPVAGLAVAVSGVSSLAFGLAHPAAVAPVPLAAYLAGSALRSIYDL
                     ERPPARHGLTPLWRALASVSAVSALSAFVLARTAYLLVRDVPPPRVVNRLGLDAEQAV
                     AGTVAMLAALFLAAGFHALGVRAGRDRSGARLVDATLVVVALAAGGTAILQKAGLLPH
                     LRAAHWATWGRAQSTFTDPSAAGVGAALLLAPCLALVASGGWPRRLAAGVGAAGLLVL
                     LADAGSRGGFIGALVSVLVLVLWAATHALAGQKPGVRRRVALSAGTLAILVALAFGAA
                     LAWPTGQGGRSALLARMSESFGKKEATTLGEPQRLLLYEGALALAREHPVTGSGLGTF
                     RSRFPDAARDLLGTDVSFTDHPPSLYLGVLAETGLAGTLVLALLLAGLLPALGGALSF
                     GGARSEESLRAAGAGAAVVGLLVVFLFGSHLVYGEIAALAGLLTSRLPAPPEGRSSRM
                     LGHVLPVAVAGASVLLLGGALARGVETWSADAAFAHAPAAGLWSEEREPDGRPFRWTS
                     RAAALRVDAPAGRPSALRIPVRNARPDGEAVRVRVYWNDRSRGTVELPHGGWKALRLD
                     VDGPGVVRLAVDRTILPPSRADRRRLGVETGAPAWAP"
     gene            5318..6301
                     /locus_tag="P5164_09390"
     CDS             5318..6301
                     /locus_tag="P5164_09390"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRY44145.1"
                     /translation="MGAVTEVRFHSSDPRRRPRAWNIDGNAGRVVGALLVLAGTLVAI
                     GLFGAPDLVAYVVRSAERLTIRATARRGLLAFDSVRLRFEKLDKRVRTDELFLARMAV
                     VLDLPSPSGLVATGEAAPAATPDALEAEVHRLGRRLRAMEGFRRTLASAKTEEPGRIP
                     SRSPVEPSSAVPVHLFGPRVSPLTHRPEFHAGLGLAAPEGTPVLAPAGGRVVFAGRVP
                     GSAGAEWRSFRNVVVIAHGERTRTVLGYLGEIRVRRGQVVRRGQEVGVVGVNRFSPTP
                     QLHYGVWRRNGARWTPVDPRLHVLDAEWIGARDLATPVVPPASLELPPAFR"
     gene            complement(6324..7478)
                     /locus_tag="P5164_09395"
     CDS             complement(6324..7478)
                     /locus_tag="P5164_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRY44146.1"
                     /translation="MDFSRASLDREFPSRLSTVQMNHAAVSPLPARAADALREYADLV
                     STRGPLAFPELEARVKMLREEGARLLGVTPELGGASSVSIVPNTTYGLALVAGGLDWR
                     PGDVVVTTESEFPANLAPWLDLARFGVVVRRVPTRDGAYGAEEVFAACDERTRLVSLS
                     LVAYHTGFVAPAAEVSAFCRERGIAFGLDAIQAAGAIPVDVAALGADYLSADGHKWML
                     GPEGCGLFFTTPAFRARLRAPSGWLNLKRTHPTQYELGANPEYAEDATKFEIGALPMP
                     GVFALHASIGLLLEAGLGEVGRRISGVLDLLAEGLPKVGFAPILHGPAVRSGILAARP
                     PAGVDARRAMRHLEGGGIACSARAGFLRLSPHFGNEPEDAEKVLGLLAKL"
     gene            7572..7841
                     /locus_tag="P5164_09400"
     CDS             7572..7841
                     /locus_tag="P5164_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HRY44147.1"
                     /translation="MPLNIKDPVTEKLAAEVARLAGETKTGAIRRSLLERKERLALRV
                     VRPDREADLVRFLEEEVWPLLPKGGSRRLTKRQREALLGYGPEGV"
     gene            7838..8224
                     /locus_tag="P5164_09405"
     CDS             7838..8224
                     /locus_tag="P5164_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRY44148.1"
                     /translation="MILDSSALVAVLLREPGWQALLGRIAADEAAAVGAPTLAEAGLV
                     LTARLGRDARRLLSAFVREAGITVLPFVEEHARVAVEAYARYGKGRHPAALNFGDCLT
                     YAVARLSGQPLLCVGDDFPRTDLPIA"
     gene            complement(8232..>9202)
                     /gene="aroC"
                     /locus_tag="P5164_09410"
     CDS             complement(8232..>9202)
                     /gene="aroC"
                     /locus_tag="P5164_09410"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRY44149.1"
                     /translation="LGSPLAMTVENLDHGVWEGVMGPLEIDAQAAAKRRLSSPRPGHA
                     DLAGGLKYGRRDLRDVLERASARETAVRVAAGAVCRQLLAACGVEVKSGVLSVGPVSV
                     EEPREGFSFETLAKVDDASPFRAVDPALEEKMTQAVDAAAKKGDTLGGTILVAARGVP
                     PGLGSHVSWDRKLDGRIGQAMLSIPSVKAVSIGSGVSNAFAPGSLAHDPISWSEERGF
                     FRERNRAGGLEGGITNGEDVLVVLFQKPIATLREGLPSVEVDTKAPHRAQYERSDVTA
                     VPACGVIGEAMLALVLADALLEKTGGDAMTEVRRNLEAFLAAQRAF"
BASE COUNT          936 a         3581 c         3407 g         1278 t
ORIGIN      
        1 cttcgagccg ctcacgttcg aggacgtgat ggcggtgatc gagaaggaga agccggccgg
       61 cgtcctcgtc cagttcggcg ggcagacgcc gctgaagctc gcccatccgc tgaaggaggc
      121 gggcgtgccg atcctcggca cctcgcccga cgcgatcgac cgggccgagg accgcgagcg
      181 cttcggcgag ctcctccgcg agaccgggct cgcggccccg ccgtggggga cggcgcgcga
      241 cgtggccgag gggctcgcgg ccggcgcgaa gctcggcttc ccgctcctcg tccggccgag
      301 ctacgtcctc ggcggccgcg cgatgcggat cgtctacgac gacgcggcgc tccgcggcgt
      361 cctcgaggag gccctcgagg ccttcccggg gcgccccgtg ctcctcgacc gcttcctcga
      421 ggacgccttc gagctcgacg tcgacgccgt cggcgacggg acggacgtcg tcgtcgcggg
      481 ggtgatgcag cacatcgagg aggcggggat ccactcgggc gactcctacg ccgtcattcc
      541 cccctaccgc cgcgtcccgc gggagtcgct cggcgagatc cgcgaggcga cgcgccgcct
      601 cgggcaggcc ctcggcgtcg tcggcctgat gaacgtccag ttcgcgctcc gcgaggggaa
      661 gctcttcgtc ctcgaggtga acccgcgcgc ctcgcggacg gtcccgttcg tcgcgaaggc
      721 gacgggcgtc ccgctcgcgc ggatcgccgc gcgcctctgc ctcgggcaga cgctcgagga
      781 ggtcggcctg acgaaggagc cgccggtccc cggcgtctcg atcaaggcgc ccgtcttccc
      841 gttccgccgc ttcgaggggg tcgacacgat cctcggcccc gagatgcgct cgacgggcga
      901 ggtgatgggg cgcgaccgga gcttcggcct cgcgttcctg aaggccgcgg cgggcgcggg
      961 agtccgcctg ccggagacgg gcaccgtctt ctgctccgtc cacgacggcg acaaggaggc
     1021 gctcctgccg atcgcgcggg agctctccgc gctcggcttc cgcctctcgg cgacgggcgg
     1081 cacggcggcg ttcctccacg cggcggggct cgaggcgacg ccggtcctga aggtgaacga
     1141 ggggcggccg aacgtggtcg accggatgat caacggagag atccagctcg tcgtcaacac
     1201 tcccctcggg aaggagtcgt tctacgacga ggtcgcgatc cgccggaacg cgctcgagcg
     1261 ggacatcccg tgcctgacga cgctcaccgc ggccgcggcg gccctcgagg cgatccgcgc
     1321 ccgggcgggg gggccgccgt cggtcacgcc gctccaggac gcgggggcgt agtggacggg
     1381 cgccgcgggc gccttctccc gctcgcggcc ttcgcggcgc gggcctcgct cgcgctggct
     1441 ctcctggcgc tcgcgttcgc ggctctccgg atcccctggc aaccggcccc gatccccctt
     1501 ctcgcattcg cggcgctggt gctgggcgcg tccctcgcgc ggccgaacgt cgcggtgctc
     1561 ctttcggccg cggcgctccc gctcctgccg atcctcggga acctcttcgg cgggccctgg
     1621 gtggcgccgg ccgagctcgt cctcctcctc ctcgcggcgg cggtgctcct ccggggcggg
     1681 tctccggccc tctccgcgcc gctcccccgg accctcgcga tctcgacggc ggcgatggcg
     1741 gccggcgcgt tcgcggcgac gctcgcggcg gtggcgccgg ccgagctccc cgcggcggtg
     1801 cgcgagcttt cgagctcctt cttctcggcg gggagcgatc acgccgcggc cccgctccgc
     1861 gcggcgctcg tccacgcctg cggcctgctc ggcttcgccc ttttccgtcg cgtcgccgcg
     1921 gcccgcggag aagggacgcc gctcgcggcg ctcgtcgcgg ggctcgccct cgtcggggcg
     1981 ttcgccgtcg tcgaggcggc gacggggctg aagctctgga gccccgcgca ctacgaggcg
     2041 ctctccgccg ggctccgggt cccctcgacg ctccccgact acaacgcgac cggcgccgcc
     2101 atggcgctgg ggctcttccc ggcgctcgcg ctcgcgtacg cggcgcgcgg cgcgcggcgg
     2161 ctcgcgtggg ccctcgccgc ggggctcctt ctcgcgggcc tcctcctctc cggctcgcgc
     2221 acggcctggc tggccgcgct cctcgcgggc ggcgcgaccc tcctcggagc gctccacgcg
     2281 cgccgaaagg aggcgcgcgg cgggagccgt cggctcctgc tcggggcctc gctcgcggcc
     2341 ggcctcgccg tcgcggcggt ggcggcgtgg ccgggcgacg tcggagcgct cctccgccgg
     2401 cgcgccgcga cgctcctcga gccggaggcg gcgctgaagg cggtccgggc ggggcgcctc
     2461 ggcttctgga cggcgggtgc gcggatggtc gccgagcatc ccgcagccgg cgtcggcccc
     2521 ggaagggtgc cggcccgctt cgacgacttc tgcgacccgg gctttccggt ccgcaacgag
     2581 aacctccaca actacttcct ccaggcggtc gcggagaacg gcgtccccgg cgggctcctc
     2641 gtcctcctgc cgttcgtccc gctcgggctc ctcctcggcc gttccctcgc gagcggggcc
     2701 gcgttcgtct cgccggccgc cgcgctcgcg cccggactcc tcgcgttcac ggcgaccggt
     2761 gtcgcctcgc acccctggct tctcccggag cttcagctcc tcttctggag cgcggcggcg
     2821 ctcctcccgg gggcggcgag aggcccggcc ccggagcccc gcttccggcg ggcgggcgtc
     2881 ctcgcgctcg cgaccctcgc cgcctggggc ggattcgtcc tcctcgcgcg tccgggccag
     2941 gagcgggggc gctggggcgc gggcgagtgg tcggccgagg gagcggagaa gccgtacttc
     3001 tgggtcggcc cgcgtgcgct cgtcccggtg gaggtccccg gcggggagcg cctgagcgtg
     3061 cggctgcggg ggatgcagga ggggctcggg cgccggccgc tcgtcgtcgc gcttcgcctc
     3121 gacggagggc cggtccggaa ggtctccgtc ccgacgcgcg cgtggcagga tgtcgtcgtg
     3181 gagcgcggcc ctcttcggcc cggcatcgcc ccggcgcgcg gcggtctcct cgcggtcgag
     3241 gcgagccgcg gtttcctgcc ttcgggggac gggcgcggcg accgtcgcgt cctggccctt
     3301 cagctcgccc ggccgcccct gcgtccggcc ccggaggcga tgccgtgaac cgtccgagcc
     3361 ccgtcgccgg gatcctcgcc ttcctcggct gggtggtcct cgcggccctc tgctccgtcg
     3421 tcctcctgcc gccgctcccg tcgcttcgaa cggcggtctg gggggcgtcg gtcgtcctct
     3481 tcgcggccgc cgtcctcgct ccgctccggt cgctccccgt cgcggggctc gcggtcgccg
     3541 tctcgggcgt ctcctccctg gcgttcggcc tcgcccatcc ggccgccgtc gcgcccgtgc
     3601 cgctcgcggc ctacctcgcc ggctccgccc tgcgctcgat ctacgacctg gagcggccgc
     3661 cggcccgcca cggcctgacg ccgctctggc gcgccctcgc gtcggtctcg gcggtctccg
     3721 ccctctcggc gttcgtcctc gcgcggacgg cctacctcct cgtccgcgac gtcccgccgc
     3781 cgcgggtcgt caaccgcctc ggcctggacg cggagcaggc cgtggccggg accgtcgcga
     3841 tgctcgccgc cctcttcctc gcggccggct tccacgcgct cggcgtgcgg gcggggcgcg
     3901 atcggagcgg cgcgcgcctc gtcgacgcga cgctcgtcgt cgtcgctctc gccgcgggcg
     3961 ggacggcgat cctccagaag gccgggctcc tcccgcacct tcgcgcggcg cactgggcga
     4021 cctgggggcg ggcgcagtcc accttcaccg acccgtccgc cgcgggcgtc ggcgcggcgc
     4081 tccttctcgc cccgtgcctc gcgctcgtgg cctcgggggg gtggccgcgc cgcctggccg
     4141 cgggagtcgg cgccgccggg ctcctcgtcc tcctcgccga cgccggctcg aggggcggct
     4201 tcatcggagc gctcgtctcg gtcctcgtcc tcgtcctctg ggcggcgacg cacgccctcg
     4261 ccgggcagaa gccgggcgtg cggcgccgcg tcgctctttc ggccgggacg ctcgcgatcc
     4321 tcgtcgcgct cgcgttcgga gccgcgctcg cgtggcccac tggacagggg gggcggagcg
     4381 cgctcctcgc gaggatgtcg gaatcgttcg ggaagaagga ggcgacgacg ctcggcgagc
     4441 cgcagcggct cctcctctac gagggggccc tcgcgctcgc gcgggagcac cccgtcaccg
     4501 gcagcggcct ggggacgttc cggtcgcgct tcccggacgc cgcgcgcgac ctgctcggga
     4561 ccgacgtctc gttcaccgac caccctccgt ccctctacct cggcgtcctc gcggagacgg
     4621 ggctcgccgg gacgctcgtc ctcgcgctcc tcctcgccgg cctgctgccg gcgctcggcg
     4681 gcgccctctc gttcggcggg gcgcgctcgg aggagtctct ccgcgcggcc ggcgcgggag
     4741 ccgcggtcgt cggcctcctc gtcgtcttcc tcttcggctc gcacctcgtc tacggggaga
     4801 tcgcggcgct cgcgggcctc ctgacgtcgc ggctgccggc cccgcccgag ggacgctcgt
     4861 cgcgcatgct gggccacgtc cttccggtcg ccgtcgccgg cgcgtcggtc ctcctcctcg
     4921 gcggagcgct cgcgcgcggc gtcgagacgt ggagcgccga cgccgcgttc gcccacgcgc
     4981 cggccgcggg cctctggagc gaagagcgcg agcccgacgg ccgtccgttc cggtggacct
     5041 cccgcgccgc ggcgctccgc gtcgacgcgc cggccgggcg tccctcggcc ctccggatcc
     5101 cggtccgcaa cgcccgtccc gacggcgagg cggtccgggt ccgcgtctac tggaacgacc
     5161 gctcccgcgg gacggtcgag ctgccgcacg gcggctggaa ggcgctccgc ctcgacgtcg
     5221 acgggccggg cgtcgtccgg ctggccgtgg accggacgat cctccccccc tcgcgggccg
     5281 accgccgccg cctcggagtg gagaccggcg cgccggcgtg ggcgccgtga cggaggtccg
     5341 cttccactcg agcgacccgc gacgccgccc gcgcgcctgg aacatcgacg ggaacgccgg
     5401 tcgcgtcgtg ggggcgcttc tcgtcctcgc cgggacgctc gtcgcgatcg ggctcttcgg
     5461 cgctccggac ctcgtcgcct acgtcgtccg ctcggcggag cggctgacga tccgggccac
     5521 cgcccgccga ggccttctcg ccttcgactc ggtccgcctt cgcttcgaga agctcgacaa
     5581 gcgcgtccgg acggacgagc tcttcctcgc gaggatggcc gtcgtcctcg acctcccgag
     5641 cccctccggg ctcgtcgcga cgggcgaggc cgcgcctgct gcgacgccgg acgcgctcga
     5701 ggcggaggtc caccgcctcg ggcggcgcct ccgcgcgatg gaaggttttc gccgcaccct
     5761 cgcttccgcg aagacggaag agccgggacg gatcccctcc cgctcccccg tcgagccgtc
     5821 gtcggcggtc ccggtccatc tcttcgggcc gcgggtctcg ccgctcacgc accgccccga
     5881 gttccacgcg ggcctcggcc tcgcggcccc ggaagggacg ccggtcctcg ccccggcggg
     5941 cggacgggtc gtcttcgccg gccgagttcc ggggagcgcc ggggcggagt ggaggagctt
     6001 ccggaacgtc gtcgtgatcg cccacggcga gaggacccgg acggtcctcg gatacctcgg
     6061 ggagatccgg gtccggcgcg gccaggtcgt ccggcgcggt caggaggtcg gggtggtcgg
     6121 ggtgaaccgc ttctcgccga ctccccagct ccactacggc gtctggcggc ggaatggcgc
     6181 gcgatggacg cccgtcgacc cgaggctcca cgtcctcgac gcggagtgga tcggggcccg
     6241 ggacctcgcg acgccggtcg tcccgccggc gagcctcgag ctgccgcccg cgttccgctg
     6301 agcctcgcgg cggaagcgcc ccgtcagagc ttcgcgagga gcccgagtac cttctccgcg
     6361 tcctccggct cgttcccgaa gtgcggagag aggcggagga acccggcgcg ggccgagcag
     6421 gcgatgccgc cgccctcgag gtggcgcatc gcccgacgcg cgtcgacgcc cgccggcggg
     6481 cgcgcggcga ggatcccgga gcggacggcc ggaccgtgga ggatcggcgc gaagccgacc
     6541 ttcgggagcc cctcggcgag gaggtcgagg acgccgctga tcctccgccc gacctccccg
     6601 aggccggcct cgaggaggag gccgatcgag gcgtggagcg cgaagacccc gggcatcggg
     6661 agcgcgccga tctcgaactt cgtcgcgtcc tcggcgtact cgggattcgc gccgagctcg
     6721 tactgcgtcg ggtgggtccg cttcaggttg agccagcccg acggggcgcg caggcgcgcc
     6781 cggaacgcgg gcgtcgtgaa gaagaggccg cacccctcgg ggcccagcat ccacttgtgg
     6841 ccgtcggccg agaggtagtc ggcgccgagc gcggcgacgt cgaccgggat cgcgccggcc
     6901 gcctggatcg cgtcgaggcc gaacgcaatg ccgcgctccc tgcagaacgc cgagacctcg
     6961 gcggcgggcg cgacgaagcc ggtgtggtag gcgacgagcg agagcgagac gaggcgcgtc
     7021 ctctcgtcgc acgcggcgaa gacctcttcg gccccgtacg cgccgtcgcg tgtcgggacg
     7081 cgccgcacca cgactccgaa gcgcgcgagg tcgagccacg gggcgaggtt cgcggggaac
     7141 tccgactccg tcgtcacgac gacgtcgccc ggcctccagt cgagcccgcc cgccacgagc
     7201 gcgaggccgt acgtcgtgtt cgggacgatc gagacggacg aagccccgcc gagctccggc
     7261 gttacgccga ggaggcgggc tccttcctcg cggagcatct tcacgcgcgc ctcgagctcg
     7321 gggaacgcga gcggtcccct cgtggagacc aggtcggcgt actcgcggag cgcgtccgcg
     7381 gcgcgcgccg ggagcggcga gaccgccgcg tggttcatct ggaccgtcga gagcctcgac
     7441 gggaattcgc ggtcgagcga ggcgcgggag aagtccatcg tccctccggg cactccggcc
     7501 ggcacgggtc gcgccggcgg gaggaaaagg tagaattcag gtgccatggt accagaccga
     7561 ggaggccctc catgccgctg aacatcaagg acccggtcac ggagaagctc gcggcggagg
     7621 tggcccggct cgcgggcgag acgaagaccg gggcgatccg ccgctcgctc ctcgagcgga
     7681 aggagcgcct cgcgctccgg gtcgtccgcc ccgaccggga ggcggacctc gtccggttcc
     7741 tcgaggagga ggtctggccg ctcctcccga agggcgggag ccgccgcctc acgaagaggc
     7801 agcgcgaggc cctcctcggc tacgggccgg agggcgtgtg atcctcgaca gctccgctct
     7861 cgtcgccgtc cttctccggg agcccggctg gcaggcgctc ctcggccgca tcgcggcgga
     7921 cgaggccgcc gccgtcggcg ccccgacgct cgccgaggcc ggcctcgtcc tgacggcgcg
     7981 cctcggccgc gacgcccgga ggctcctctc cgcgttcgtg cgggaggcgg ggatcacggt
     8041 cctcccgttc gtcgaggagc acgcccgcgt cgccgtcgag gcgtacgccc gctacggcaa
     8101 ggggcgccat ccggcggcgc tgaatttcgg ggactgcctg acgtacgccg tcgcgcggct
     8161 ctcggggcag ccgctcctct gcgtcggaga cgacttcccg aggaccgacc tcccgatcgc
     8221 ctgaggccgg ctcagaacgc gcgctgcgcg gcgaggaacg cctcgaggtt ccggcggacc
     8281 tcggtcatcg cgtcgccccc cgtcttctcg aggagcgcgt cggcgaggac gagcgcgagc
     8341 atcgcctcgc cgatgacgcc gcaggcgggg acggccgtca cgtcggaacg ctcgtactgc
     8401 gcgcgatgcg gcgctttcgt gtcgacctcg accgacggga gcccctcgcg aagcgtggcg
     8461 atcggcttct ggaagaggac gacgaggacg tcctcgccgt tcgtgatccc tccctccagg
     8521 cctcccgcgc ggttccgctc gcggaagaag ccccgctcct cgctccacga gatcgggtcg
     8581 tgcgcgagcg agccgggggc gaacgcgttc gagacgccgg agccgatcga gaccgccttc
     8641 accgagggga tcgagagcat cgcctggccg atccgcccgt cgagcttccg gtcccaggag
     8701 acgtgcgagc cgagcccggg cgggacgccg cgcgccgcga cgaggatcgt cccgccgagc
     8761 gtgtccccct tcttcgcggc cgcgtcgacg gcctgcgtca tcttctcttc gagcgcaggg
     8821 tcgacggcgc ggaacggcga ggcgtcgtcg accttcgcga gcgtctcgaa cgagaacccc
     8881 tcgcgcggct cctcgaccga caccgggccg acggagagga cgccgctctt cacctcgacg
     8941 ccgcacgccg cgaggagctg ccggcagacg gcgcccgcag cgacccgcac cgccgtctcc
     9001 cgcgcgctcg cccgctcgag gacgtcgcgc aggtcgcgcc ggccgtactt gaggcccccc
     9061 gcgaggtcgg cgtgtcccgg cctcggcgac gagaggcgcc tcttcgcggc ggcctgggcg
     9121 tcgatctcga gaggccccat cacgccttcc cagacgccgt ggtcgaggtt ctcgaccgtc
     9181 atcgcgagcg gagagccgag cg
//
LOCUS       DAOTEZ010000151         9175 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5370_length_9175_cov_4.362061, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000151 DAOTEZ010000000
VERSION     DAOTEZ010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9175
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5370_length_9175_cov_4.362061"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2760
                     /locus_tag="P5164_09415"
     CDS             <1..2760
                     /locus_tag="P5164_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44150.1"
                     /translation="AGEASARIVPSAPVSQWPVIPAVQKRAARHVVVIELVRYGESCY
                     YCVPTSGRLVVHDSLGLEAPRDVTPGGQPADIEAAAAWEGADGTLRLFVTGHFASPLP
                     SPQAGSLLYSADGGATWAPVAVPPGSQAWAPEPVWRDAGGPIAHAVGSPIRLGTAAVP
                     FVVSVSSPDASGIWGVRADGSTYSLTPPTLAGDILGSDVGGSRFLALLRPAPPANSAQ
                     SPWTLALLDLAGNTSPIFDTTGFPWNPWMEGWIAPDGSAYLNVDWTWGGNFVSPVKPP
                     ILPSTRSLVLWKEGSLREIVSTESGTLLGIPAPDFAGAWILNRGGDATVLASHTPSGG
                     LVEAWRDGSRPRIEGVFASESGRRLLFLRRGILGSGPGSFALTVWEVGQPAPSGWDRV
                     VLSSEGTPTLVHLDVDAAAVAGAPIFADPGSPLGFAMPSGEPPAPNSPSFFQTNPLLR
                     LSLRQELVVPASARAAQGLFGASWRTDLVLRNPGASAVPVTVRFLPNPRTAPSVPDAS
                     VTVEPRAIAVVPDVLGSLFGLESGSGALRLLPAAGGAIDATSRTYTVSSGGSYGMALD
                     AEDVIAGSRLGFAQSFPAVLLGSGFRTNLIATGLSRETSRIEVLSSGGGGATSGPLSV
                     DVPAGGQVQVDDVAALAGASGSIEGSFLVTSRSGSTMTGFTVIDNATNDPAWSGPAVP
                     PGPLTIPAVVHSAGAHGAEFRTDLFFFNPDPVTRTVPVRGALWTGAIREFSTTVTLAP
                     GESKRIPDALFTLFGLTGVARLSFWSGFPDGGVAATSRTYTLLPDGSTYGMLVPPLTP
                     AQVAGAGETLEILGPTGGAGSRTNLSLVKLDPSPGRVDVRIFDESGALLDGFERELPA
                     YGGVQVDDLFRARGLGDGPKAALIQVSFAPGERYGSRLAAYATTIDNATNDPVYFAGR
                     LAAVD"
     gene            3142..3357
                     /locus_tag="P5164_09420"
     CDS             3142..3357
                     /locus_tag="P5164_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44151.1"
                     /translation="MTVEELIAQIRNLPAPDLYRLLDVIEGQVEAVSPPQASDWTPLF
                     GTLEAGQAAEILKAVGDDCRRVDQSGW"
     gene            3347..3736
                     /locus_tag="P5164_09425"
     CDS             3347..3736
                     /locus_tag="P5164_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRY44152.1"
                     /translation="MAGRYLVDTNIVVAWFAGERPLLDRFEAAAEVNASVVTLGELVY
                     GAMKSSRPRENLQRLEQLRNTVALLRCDAETARRYGELKDELRRKGRPIPENDIWIAA
                     TALQHGLTLVTRDSDFEHVEGIDREVW"
     gene            3805..4023
                     /locus_tag="P5164_09430"
     CDS             3805..4023
                     /locus_tag="P5164_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44153.1"
                     /translation="MIALLEKALAEISKLPLSQQEEVATWLLAELTDEGLWASSFARS
                     QPTLDALADEALAEHSAGLTEPLDPERL"
     gene            4308..5366
                     /locus_tag="P5164_09435"
     CDS             4308..5366
                     /locus_tag="P5164_09435"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HRY44154.1"
                     /translation="MDSLMFLVVIGTSLWVLVDAKTIGVRKGQIKGAGDMGPWGWFFV
                     CLLLWIVGFPFYLAKRGEYKKVNAAPPPLPVSAPPARGQDDSIAQLERLAQLRDRGVL
                     TEAEFQAKKSQLLAVALVTVLGLGVLTACTKDPKQLRITEANKETFLEELKPMKGLTF
                     EESGLLFQHALRKGMGEAFGQREPSPVGKTVGDLIAAERARQEKEKKETDEQERLARE
                     AKAREEALEAELRKALAFTVFAKDFLDADAMAGRYSGVIIIRCAYENLSGKDVRAFTG
                     TLRFTDLFGKEVFSSGLTISDPVSAGEKAKWTGTIEFNQFSDEHRRFRDAELANLKVV
                     WMPKQVLFADGSKLGGEE"
     gene            5426..6025
                     /locus_tag="P5164_09440"
     CDS             5426..6025
                     /locus_tag="P5164_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HRY44155.1"
                     /translation="MATEPEGARSQPDWDGLFAVAQAQSGYFTTAQAMRSGYSRPLLH
                     KYVAAGRMSRVRRGVYRLVHFPVSEHEDLVVLWLWAGQLGVFSHETALALHDLSDALP
                     GKVHMTVPSGWRRRRLRVPAGLVLHFADLGEGDLARYSAVRVTAPFRTLRDCIEAEVA
                     HDHVRQGVLQARRRGLVSAKDEARLLAELGSRGRTAAAR"
     gene            6022..6942
                     /locus_tag="P5164_09445"
     CDS             6022..6942
                     /locus_tag="P5164_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRY44156.1"
                     /translation="MTARQYSTPLAFKQALEQRLRASTTTGIAFARRRQLLVFDRFLA
                     RLAQVAGESVVLKGGLVLELRLARARTTKDVDLRMMGSPGNVLELLQEAGRLDLGDCM
                     LFEVQPDADHPEIQNEGMRYDGFRFRAECRLAGMIYGRPFGVDVAFGDPLVGEPDVVV
                     TDDVLAFAGIAPPSLRLYPVVSHIAEKLHAFTMPRPRPNSRVRDLPDLALLATTGPID
                     GAALRRAIELTFEFRGTHAVPKECPPPPEFWAAPYEAMAENDDLAWKTLEEVTRAVKV
                     FLDPALGSAECGAWEPSGWRWQRRAEAMKL"
     gene            6965..>9175
                     /locus_tag="P5164_09450"
     CDS             6965..>9175
                     /locus_tag="P5164_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016864589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HRY44157.1"
                     /translation="MKSAEVRDELIRALRLDLIGPEPGSEHEREELPHEPSRWYVGGF
                     LVPFEAPEEQKVDPTGQEELFAAGDENGADDADVPERASARKVFLPSSVGLSLLVAKG
                     ADALEAEVTWGDYRLPKTEEGEPRGKETAWTREPKRAVVALTLPEGEARAATTAVPDS
                     NGLKLVVTARNAGGVEALGLAKGTRSVAVFLVNDRKPAPEDRKDEAVAFQVRLGLRSK
                     EPLVARPNATGIDSTDWDDRVADLQYRDVFEFAVGHGVSARAVVAEDGSCREVATEWV
                     PTAAVEKVEPSRLDGVELGMEALAATKDAEALAAALAAFPARYGEWIARQRETKLEGK
                     RAEAAAILLDEAKHACARIERGLALLGEPDVFKAFQVANRAMARAARRRSARAANVAP
                     EAVEPPRWRPFQLAFLLMNLEGIARPGSSDREAVDLLFFPTGGGKTEAYLGLAAFTMV
                     LRRLRNPGLSSAGVAVLMRYTLRLLTLDQLGRVAALVCALELERAADEALGPWPFEVG
                     LWVGRAATPNRMGAKGDDDETTARSKTLKYKNDSKRWPPPIPLETCPWCGTPFTKDSF
                     SLQPSSDRPTDLRVVCVSRKCEFRGDRPLPILTVDEPIYARRPAFLIATVDKFASLPW
                     VGDVGRLFGKGEKEPLLPPELIIQDELHLISGPLGTMAGLYEIAVDRLASREEAGKRV
                     KPKVVASTATIRRAEAQVRALFGRSAVYVFPPPGPDRRDSFFARTVSQSEKEPRT"
BASE COUNT         1329 a         3117 c         3339 g         1390 t
ORIGIN      
        1 gcgggcgaag cctccgcccg aatcgtcccg agcgccccgg tgtcgcagtg gcccgtgatc
       61 ccggccgtgc agaagcgggc cgcccgccac gtcgtcgtca tcgagctggt ccgctacggg
      121 gagtcctgct actactgcgt cccgacgagc ggtcgcctcg tcgtccacga ctccctcggc
      181 ctcgaggctc cgcgcgacgt gacgcccggc ggacagccgg ccgacatcga ggcggcggcg
      241 gcctgggagg gcgccgacgg gactctccgg ctcttcgtga ccggccactt cgcctctccg
      301 ctcccgtcgc cgcaggcggg cagcctcctc tacagcgccg acggcggggc gacctgggcg
      361 ccggtcgccg ttccgccggg gtcccaggcg tgggcgccgg agcccgtgtg gagagacgcg
      421 ggcggcccga tcgcccacgc ggtcggctcg ccgatccggc tggggacggc ggccgtgccg
      481 ttcgtcgtct cggtctccag cccggacgcc agcggcatat ggggagtccg cgccgacggc
      541 tcgacgtact cgctgacgcc cccgacgctg gccggcgata tcctcgggtc ggacgtgggc
      601 ggctcgaggt tcctcgcgct gctgcggccg gcgccgcccg cgaattccgc gcagagcccg
      661 tggacgctcg cgctcctcga cctcgcgggg aatacctcgc cgatcttcga cacgaccggc
      721 ttcccatgga acccctggat ggagggatgg atcgcgcccg acgggagcgc ctacctgaac
      781 gtcgactgga cctggggcgg caacttcgtc tctcccgtga agcccccgat ccttccctcg
      841 acccgctccc tcgtcctctg gaaggagggg tctctccggg agatcgtctc gaccgagagc
      901 gggacgctgc tcggcattcc ggcgcccgac ttcgcgggcg cgtggatcct aaatcgggga
      961 ggagacgcga cggtcctcgc ctctcacacg ccctccggcg gcctcgtcga ggcgtggcgt
     1021 gacggatcgc gcccacgcat cgagggggtc ttcgcgtcgg aatccggccg gcgcctcctc
     1081 ttcctgcggc gaggcatcct cggcagcggc cccggctcgt tcgcgctcac cgtctgggag
     1141 gtcggccagc cggcgccatc cggctgggac cgggtcgtcc tgagctcgga gggcacgccg
     1201 acgctcgtcc acctcgacgt cgacgcggcg gcggtggccg gagcgccgat cttcgccgac
     1261 ccggggtcgc cgctgggctt cgccatgccg tcgggcgagc cgcccgcgcc gaactcgccg
     1321 tcgttcttcc agacgaaccc gctccttcgg ctctctctgc ggcaggagct ggtcgtgccc
     1381 gcgtcggcgc gcgccgcgca ggggctcttc ggcgcgagct ggcgcacgga cctcgtcctc
     1441 cggaacccgg gtgcgagcgc cgtgccggtc acggtccgtt tcctgccgaa ccctcggacc
     1501 gcgccttccg tgcccgatgc gagcgtgacg gtcgagccgc gcgcgatcgc cgtcgttccc
     1561 gacgtgctcg ggagcctctt cggactcgaa tcgggctccg gtgcgctccg cctcctgccg
     1621 gccgccggcg gcgcgatcga cgcgacgagc cggacgtaca ccgtatcttc gggcggcagc
     1681 tatggaatgg cgctggacgc cgaggacgtg atcgcgggca gccggctcgg gtttgcccag
     1741 agcttcccgg ctgtgctcct cggctccggt ttccggacga acctcatcgc gaccggcctc
     1801 tcgcgggaga cgtcgcgcat cgaggtcctt tcctcgggcg gcggcggagc cacgagcggg
     1861 ccgctgtccg tcgacgtccc ggccggcggc caggtgcagg tcgacgacgt cgcggcactc
     1921 gccggggcat cgggttcgat tgaaggctcc ttcctcgtca cgtcccgctc cgggtccacg
     1981 atgacgggct tcaccgtcat cgacaacgcg acgaacgacc ccgcgtggtc ggggccggcg
     2041 gttccccccg ggccgctcac gattcccgcc gtcgtccact cggccggggc ccacggggcg
     2101 gagttccgca cggacctctt cttcttcaat cccgaccccg tcacgcggac ggttcccgtc
     2161 cgcggcgcgc tctggaccgg ggcgatccgg gagttctcga cgaccgtgac gctcgcgccg
     2221 ggggaatcga agcgaatacc ggatgcgctc ttcacgctgt tcgggctgac cggcgtcgcc
     2281 cggctgagct tctggagcgg cttcccggac ggcggcgtcg ccgcaacgtc gcgcacgtac
     2341 acgctcctcc cggacggctc gacctacggg atgctcgtgc cgcccctcac ccccgcgcag
     2401 gttgccggag ccggggagac gctcgagatc ctcggcccga cgggaggggc cggctcccgc
     2461 acgaacctct cgctcgtgaa gctggacccg agccccggca gggtggacgt ccggatcttc
     2521 gacgagagcg gcgcgctgct cgacggcttc gaaagggagc tgccggccta cggaggcgtc
     2581 caggtcgacg acctcttccg cgcgcgcgga ctgggcgacg gcccgaaggc cgcgctgatc
     2641 caggtgtcgt tcgctccggg ggagcgctac ggaagccggc tcgcggccta cgccacgacg
     2701 atcgacaacg ccaccaacga ccccgtctac ttcgccggcc gcctcgcggc ggtggactga
     2761 agcccgacat cctcgtccgg aagggccgca gcccgcgcgt cgtcgccgac acgaagtgga
     2821 agcgcctcga cccgcggaag gccgacctcg gcgtctcggg cgaggacgtc taccaggtcc
     2881 tcgcctacgc ccaccgctac gccaccgaca ccgccgtcct catctacccc caccacgccg
     2941 cggtcgggcg ccccggcctc cagcgcgact tcgtcatcca gggtagggga gccccgcccg
     3001 tcgtcgtgag ggtcatgacg gtggaactgg cgaggctgga gagggtgccg ggggcggtgg
     3061 gggaggcggt cagcgccggc tgcggagacc gggcgtagta caccgccctg tatgattcga
     3121 gcggaaggag gatgcgggac gatgaccgtc gaagagctga tcgcacagat tcgaaatctg
     3181 ccggcgccgg acctgtatcg ccttctcgac gtgatcgaag ggcaggtcga ggccgtatcg
     3241 ccgccccagg cctcggactg gacgcctctt ttcgggactc tggaagcggg ccaggcggcg
     3301 gaaattctca aagccgtggg cgatgactgc cgacgggtcg atcagagtgg ctggtagata
     3361 cctcgtcgac acgaacatcg tcgtcgcctg gttcgcgggc gagcgccccc tcctggatcg
     3421 attcgaggct gccgccgagg tcaacgcctc ggtcgtgacg cttggcgagc tcgtttacgg
     3481 ggcgatgaag tcgagccgcc cgcgagagaa cctccagagg ctcgagcagc ttcggaacac
     3541 ggtggctttg ctgaggtgcg atgcggagac ggctcgccgg tacggtgagc tcaaggacga
     3601 gcttcgccga aagggaaggc cgattccgga gaacgacatc tggatcgcgg cgaccgccct
     3661 tcaacacggg ctgaccctgg tgacgcggga ctcggacttc gagcacgtcg aagggatcga
     3721 tcgggaggtc tggtagcgcg aagcggtgag cgaccggcgc tcgatcgaat cggaggccgc
     3781 aagctggagg tggaggccgt tgtcgtgatt gcccttctcg agaaggctct tgccgagatc
     3841 tcgaagctgc ccctgtccca gcaggaggag gtggcgacgt ggctcctggc ggagctgaca
     3901 gacgaggggc tctgggcgtc gtcgttcgcg aggtcgcagc cgacgctcga cgccctcgcc
     3961 gacgaggcgc tcgccgagca ttcggccgga ctcaccgagc cgctcgaccc cgagcggctt
     4021 tgaagtcgcg ggcgacgaga tcgtctggtt ctggatcggg tcccacgacg actgcgagac
     4081 attgatctcg cggcgattgg aacggtttcg cgctccatcg ccggcattcg aagggagtgg
     4141 agctgccggc tccgcggctg gcttgtggtg ggccggctcg gacgtcattc gtcccggtgt
     4201 cggatgcaga ttcctcgacg tttgcggtcg gggcgtcgtg ttccaggccg gcccgcgggg
     4261 cggattccac gttcctggcg gagtgtcccg acccggagga ggagttcatg gacagtctga
     4321 tgttcctcgt cgtcatcggc acgtcgctgt gggtcctcgt ggacgcgaag acgatcggag
     4381 tccggaaggg gcagatcaag ggggcgggag acatgggccc gtgggggtgg ttcttcgtct
     4441 gcctcctgct ctggatcgtc gggttcccct tctacctggc gaagcgaggc gagtacaaga
     4501 aggtgaacgc cgctccgccg ccgctcccgg tgagcgcgcc gccggcccga ggccaggacg
     4561 actcgatcgc ccagctcgag cgcctcgcgc agcttcgaga caggggagtc ctcacggagg
     4621 cggagttcca ggcgaagaag tcgcagctcc tcgccgtcgc cctcgtcacc gtcctcggcc
     4681 tcggcgtcct gacggcctgt acgaaggacc cgaagcagct gcggatcacc gaggcgaaca
     4741 aggagacctt cctcgaggag ctgaagccga tgaaggggct gacgttcgag gagtccggcc
     4801 tcctcttcca gcacgcgctc aggaagggaa tgggcgaggc gttcggccag agggagccct
     4861 ccccggtcgg gaagacggtg ggcgacctga tcgccgccga acgggcgcgc caggagaagg
     4921 agaagaagga gaccgacgaa caggagcgtc tcgcgaggga ggcgaaggcc agggaggaag
     4981 ccctcgaggc cgagcttcgg aaggcgcttg cgttcacggt ctttgcgaag gactttctgg
     5041 acgccgacgc gatggcgggc cggtacagcg gcgtcatcat catccgctgc gcctacgaga
     5101 acctctcggg gaaagacgtc cgggccttca cgggaacgct tcgattcacc gacctcttcg
     5161 ggaaggaggt cttctcctcg ggtctcacga tctccgaccc cgtctcggca ggcgagaagg
     5221 cgaagtggac gggaaccata gagttcaacc agttcagcga cgagcaccgc cgcttccggg
     5281 atgcggagct cgcgaacctc aaggtggtct ggatgccgaa gcaggtcctc ttcgcggacg
     5341 ggagcaagct cgggggcgag gagtgagggg gcgagggggc ctctcggctg cacagacgtt
     5401 aaccgtgggt taacattcgt gcgacatggc caccgagcct gaaggagcac gaagtcagcc
     5461 ggattgggat gggctcttcg cagtcgcgca ggcccagagc ggctacttca cgacggccca
     5521 ggcgatgcgg tcaggctatt cgcgtccgct cctccacaag tacgttgcgg cggggaggat
     5581 gtcgcgcgtg cgacgaggcg tgtaccggct cgtccacttc ccggtcagcg aacacgaaga
     5641 cctcgtcgtt ctctggctct gggccggcca gctcggggtc ttctcccatg agacggccct
     5701 cgcgctccac gacctctccg acgctcttcc cggaaaggtc cacatgacgg tgccctcggg
     5761 ctggcggcgt cggcggctca gggtgccggc cggcctggtg cttcacttcg ccgacctcgg
     5821 cgagggtgac ctcgccaggt attcggcggt tcgcgtcaca gcacctttcc gcacccttcg
     5881 ggactgcatc gaggctgaag tcgcccacga ccacgtgcgg caaggagtcc tccaggcgcg
     5941 ccgacgcggg ctcgtctcgg cgaaggatga agctcgactc cttgccgagc tgggcagccg
     6001 gggtcggacg gcggccgcgc ggtgactgct cgccagtact cgacgcccct ggccttcaag
     6061 caggccctcg agcaacggct ccgggcctcg acgaccacag gcatcgcctt cgctcgtcgc
     6121 cggcagctgc tcgtcttcga tcgcttcctc gcccggctgg ctcaggtcgc cggcgagtcc
     6181 gtggtgctca agggagggct cgtcctcgag ctgcgtctcg cgcgggcgcg aaccaccaag
     6241 gacgtcgacc tccgcatgat gggctcgccc ggcaacgtgt tggagctgct ccaggaggca
     6301 ggtcgcctcg acctcggtga ctgcatgctg ttcgaagttc agccggacgc cgatcacccc
     6361 gagatccaga acgaggggat gcgctacgac ggcttccgct ttcgagcgga gtgccgcctc
     6421 gcgggcatga tctacggccg gccgttcggc gtcgacgtcg ccttcggcga cccgctcgtg
     6481 ggcgagccgg acgtcgtcgt gaccgacgac gtcctcgctt tcgcgggcat cgcccctccg
     6541 tcgctccggc tctatcccgt cgtctcgcac atcgcggaga agcttcatgc gttcacgatg
     6601 ccccgaccga ggccgaactc acgggtccgg gatcttccgg acctggctct cctggcgacg
     6661 accggtccga tcgatggggc cgctctccgt cgggccatcg agctgacctt cgagttccgc
     6721 gggacacacg ctgttccgaa ggagtgtccg ccgcctcccg aattctgggc ggctccctat
     6781 gaggccatgg ccgagaacga cgatctcgca tggaagaccc tcgaggaagt gacgcgggca
     6841 gtgaaggtct tcctcgatcc cgctctcggt tccgccgagt gcggagcctg ggagccttcg
     6901 ggctggcgct ggcagaggcg cgccgaagcg atgaagctgt gacgggccga ggtgctattt
     6961 gaaggtgaaa tccgctgaag tccgcgacga gctgatccgt gccctgcgcc tcgacctgat
     7021 cgggccggag cccgggagcg agcacgagcg agaggagctg ccgcacgaac cgtcgcgctg
     7081 gtacgtgggc gggttcctgg tgcctttcga ggcgccggag gagcagaagg tcgacccgac
     7141 ggggcaggag gagctgttcg cggcgggaga cgagaacggg gcggacgacg cggacgtgcc
     7201 cgagcgggca tcggcgagga aggtttttct gccgtcgtcc gtcggtctga gcctcctcgt
     7261 cgcgaaaggg gcggacgcgc tcgaggccga ggtgacgtgg ggcgactacc ggctgccgaa
     7321 gacggaggag ggcgagccgc ggggcaagga gactgcgtgg acccgggagc cgaagcgggc
     7381 cgtcgtcgcg ctgacactgc ccgagggcga agcgagggcg gcaacgacgg ccgtgccgga
     7441 ctcgaacggc ctgaaactcg tcgtcacggc acggaatgcg ggcggggtcg aggcgctcgg
     7501 cctcgcgaag gggacgcgga gcgtcgccgt cttcctcgtg aacgaccgca agccggcccc
     7561 ggaggatcgg aaggacgagg cagtcgcctt ccaggtgcgg ctcgggctgc ggtcgaagga
     7621 gccgctcgtc gcgcggccga acgcgaccgg gatcgactcg accgactggg acgaccgcgt
     7681 ggccgacctc cagtaccgcg acgtcttcga gttcgccgtg gggcacgggg tctcggcgcg
     7741 agcggtggtc gcggaagacg gaagctgccg cgaggtggcg accgagtggg tgccgacggc
     7801 cgccgtggag aaggtggagc cgtcgcgtct cgacggcgtg gagctgggga tggaggcgct
     7861 cgcggcgacg aaagacgccg aggctctcgc ggccgcgctc gcggcgttcc cggcacggta
     7921 cggcgagtgg atcgcgcggc agcgggagac gaagctcgaa gggaagcggg ccgaggcggc
     7981 ggcgatcctc ctcgacgagg cgaagcacgc ctgcgcgcgg atcgagcggg gcctcgcgct
     8041 tctcggcgag cccgacgtct tcaaggcgtt ccaggtcgcg aaccgcgcga tggcgcgggc
     8101 ggcgcggcgg cggagcgcgc gggcggcgaa cgtcgcgccg gaggcggtgg agccgccgcg
     8161 gtggcgcccg ttccagctcg cgttcctcct gatgaacctc gaggggatcg cccggcccgg
     8221 ctcgagcgac cgcgaggcgg tggacctcct cttcttcccg acaggcggcg gcaagacgga
     8281 ggcgtacctg ggcctcgcgg ccttcacgat ggtgctccgc cggctgcgca acccggggct
     8341 ctcctcggcc ggggtcgccg tgctgatgcg ctacacgctg cggctcctga cgctcgacca
     8401 gctcgggcgc gtggcggcgc tcgtctgcgc gctggagctg gagcgtgcgg ccgacgaggc
     8461 gctcgggccg tggccattcg aggtggggct ctgggtgggg cgcgccgcga ccccgaaccg
     8521 gatgggggcg aagggtgacg acgacgagac gacggcgcgg tcgaagacgc tgaagtacaa
     8581 gaacgacagc aagcgctggc cgccgccgat cccgctcgag acctgcccct ggtgcgggac
     8641 gccgttcacg aaggactcct tctcgctcca gccgagctcc gaccggccga cggacctccg
     8701 cgtcgtctgc gtgagccgga agtgcgagtt ccggggcgac cgaccgctgc cgatcctgac
     8761 cgtggacgag ccgatctacg cgcggcgccc ggcgttcctc atcgccaccg tggacaagtt
     8821 cgcgagcctg ccgtgggtag gcgacgtggg gcggctgttc gggaaggggg agaaggagcc
     8881 gctcctgccg cccgagctga tcatccagga cgagctgcac ctcatctcgg gcccgctcgg
     8941 gacgatggcg gggctctacg agatcgccgt cgaccggctc gcgtcccgcg aggaggcggg
     9001 gaagcgggtc aagccgaagg tcgtcgcctc gacggcgacg atccggcggg cggaggcgca
     9061 ggtacgggcg ctcttcggac ggagcgccgt ctacgtcttc ccgccgccgg gaccggaccg
     9121 gcgcgactcg ttcttcgcgc gaacggtttc gcaatccgag aaggagccgc ggacg
//
LOCUS       DAOTEZ010000152         9124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5399_length_9124_cov_6.284155, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000152 DAOTEZ010000000
VERSION     DAOTEZ010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9124
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5399_length_9124_cov_6.284155"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="P5164_09455"
     CDS             complement(<1..258)
                     /locus_tag="P5164_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HRY44158.1"
                     /translation="METTYRPTTLGELVEAGYRHRSVRDEMRENLLAMLARKENPFPG
                     ILGYERTVVPALVNAVLARHDVVLLGLRGQAKTRILRSLVNL"
     gene            complement(290..1672)
                     /locus_tag="P5164_09460"
     CDS             complement(290..1672)
                     /locus_tag="P5164_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRY44159.1"
                     /translation="MNERTPRATLLVVDDEKHQRESLRMILEDEGYRVTAAADGREAL
                     ALAAETHPEVVLTDLKMPGLSGLDLVRALVEGPLPPRVILITAHGSVERAREAHRLGA
                     FDYMSKPVVADELLFRVERALESHRLAERALRLERRLKGDGLEAMVGESSAMREVFRL
                     VEKIAPTQSTVLVRGESGTGKELVARAIHSRSARAARAFQAINCAAIPESLLESELFG
                     HERGSFTGADARKTGLFEAASGSTLFLDEIGDLSLPLQGKILRALQEREIRRVGGNET
                     IAIDVRVVAATNRELESMMKAGQFREDLFYRLNVIPIVLPPLRERSDDIPALVHRFLD
                     KANGTHGTAVADIDPAALDLLVRHPWPGNIRQLESVVERAVLLCEGTTVRPADLPLEI
                     RLKTAPAARPYGFEIPPEGIDIEELERQLIVQAMERSAWVIARAARLLGLTYRTLQYR
                     LEKFGIRKPA"
     gene            complement(1682..3154)
                     /locus_tag="P5164_09465"
     CDS             complement(1682..3154)
                     /locus_tag="P5164_09465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY44160.1"
                     /translation="MSPLRNPFRGPSLTLRLGLALTGILVAAAAAQTVLYLRSQNQML
                     AQADASYKTLATAIQAAATRIGPGGSRDPEALEEFRERLKQKGVREIRIKQGGELLPD
                     GAPAGTATTARRRARPRSGPQDIEIEGVVGEGAGSGEIVVPLVIERRYLGQVTIKYSL
                     ENIRVELEDNFRRGLYALLGVFGVGLVVILVVTRNATRPVEAVAEAARQVADGQLDVV
                     VPVDRADEIGQLAIAFNRMTAALRERQELLARLAAAEKRAEIGHLAAGLAHEIKNPLN
                     ALSLGLDVLRRRHRPAEQAAAAEQAERIDALRGEIDRLAALINNFLAFGRPLTLSILP
                     VDAAALVRDTLADLAETAGQVRVRFSTDLPAGLPPVAADGSLLKSVIWNLAQNGIQTM
                     EKEGGTLRVAARLADGTVDEGPRVVLTLEDEGPGIDAAHLPRLFEPWFSTKEGGVGLG
                     LAMVKRIVEEHGGRVEAGNRESGHGALFRISLPVVAAPSE"
     gene            3307..5262
                     /gene="acs"
                     /locus_tag="P5164_09470"
     CDS             3307..5262
                     /gene="acs"
                     /locus_tag="P5164_09470"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HRY44161.1"
                     /translation="MSDGTPEKALDTLLLEHRTFDPSPEFRRSAVARDPGVYAQAAAD
                     PESFWASFARELEWIRPYDRVLEWKAPDARWFVGGRLNVSANCLDRHLHSARRHKAAL
                     VWEGEPGESRIYTYQQLHHHVCKFANVLKRLGVGKGDRVCIYLPMVPEAVIAMLACAR
                     IGAVHSVVFGGFSADALKDRIQDAGAKLLVTADGGWRRGQIVQLKKMADEALKECPTV
                     QAVVLVKRGDFPCHVKEGRDHWWHRLMDEVRGDCPYESMDSEAPLFILYTSGTTGKPK
                     GILHTTAGYLTGVYGTTKWVFDLKDEDLFWCTADVGWVTGHSYVVYGPLAAGATVLMY
                     EGAPDWPDKDRFWQMIEKYGVTVFYTAPTAIRAFMKWGTHYPEKWDLSSLRLLGSVGE
                     PINPEAWMWYQEHIGRRRCPIVDTWWQTETGHIMITPLPGLTRTKPGSATMSFPGVDA
                     CVLNEKGEEVTEGGGYVAVRKPWPGMLRGIWGNRERFVKAYWSKWDGVYFPGDGVRKD
                     EDGYFWFLGRVDDVLNVAGHRIGTAEVESALVDYPLVAEAAVVGIPHDLKGTAIAAFV
                     TLKSGHHGSDELVQDLKEHVIEKIGAIARPDKILFSADLPKTRSGKIMRRLLRDIAEG
                     RVLGDTTTLADPNVVKQLRDQYEGNES"
     gene            complement(5282..6004)
                     /locus_tag="P5164_09475"
     CDS             complement(5282..6004)
                     /locus_tag="P5164_09475"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRY44162.1"
                     /translation="MRRFRPHVAAEQAGVKELPMALLVGFAVGLFGAIPPGPVNVNVI
                     RKASRAETREALRVALGAALVDTVICGFVAMGFGWALEKVVANPWTRLGLALFLLASG
                     LKILVLDRKRDAERTARPGLTAPPKEVSGFRFPLLVGVLQGAANPALFVNWTLLISFL
                     VGHRILPTGLLPAGVFAVGVGLGVFAWFAILVELVERLRNHPSGEWIHKSSFAAGVLL
                     LGFGLWFSWRTVAELVSGIWIP"
     gene            6045..6683
                     /locus_tag="P5164_09480"
     CDS             6045..6683
                     /locus_tag="P5164_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44163.1"
                     /translation="MKACVPFLALALALPAAAQEERLEFDPQVGMTGTRVTVTSPLPK
                     GAVVRFGTRVVPLLQEPGRRPSFVVPEGAKSAFIEASLDGKPFSRSAVPFVVAGVSLV
                     GVQKLVGLREAIDVFGYSDPTPEGGGAPEQRAKKILSFGDGDLITIGEAPAPAPPLWV
                     IGNDAASAAMRGMGPPGFLLTARPPTRRIIVIPPPPPVPTPVPTPTPSPSPG"
     gene            6814..7413
                     /locus_tag="P5164_09485"
     CDS             6814..7413
                     /locus_tag="P5164_09485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRY44164.1"
                     /translation="MVLPVNGPSEARDWPGDEKAVARFLGGDPTGFEQIVRHYSGMVF
                     SLAARLVGPWEAEEVVQETFLRAWRGLETFRGDSSLKTWLYTIALNRAKARQGTLARM
                     RKVFASRRAAEEEDRPWPGEEAPDPSASPEEQTLALEQRTRLRRAIRNLPEEFRHAVV
                     LRDLEGLSYDEIARVLGVPIGTVRSRIARGRAALLEELS"
     gene            7410..8447
                     /locus_tag="P5164_09490"
     CDS             7410..8447
                     /locus_tag="P5164_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44165.1"
                     /translation="MSANPQPRPLVTGDLDLLISRSLDGDLPGEEERDLKSLLAADAA
                     ARARFDAMARLVDRLEELPEAETPFALATRVGSQVEADTQGFAATLHKYGFYFRPATF
                     GVIAAGLVVVAVIATLRNPAKPVATVAEVKALPAPAAGEDRMTVYLATTEKPKEEAAA
                     PADPAPAASGVPAEAKGVGRPEPVLAAKAESPVGRDQAGFAPERDAAANDRLASATSA
                     SEGRAAAGSAEVVRQETEAAPARLRAAAPAPAPPSAERPSPRLDLVGSSSAGLRLVAP
                     ARLDGLPGPFDGSYRLELNASGRVSEVVRLFGGSGTEPPALPDRLRTLSFASSNGAPL
                     AGPVDVRVRLP"
     gene            8576..8998
                     /locus_tag="P5164_09495"
     CDS             8576..8998
                     /locus_tag="P5164_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44166.1"
                     /translation="MKRTQAARVRKALDFVKKRLKLDLEFVVEPGEVHEQFGLLDIER
                     SMRGTEDRKVWVVSFDPELVSKESLAALRRHAFHEVLHALTWPLFDEAEAAIRSVPDS
                     RLRKELMDRSIDARENVVYELERKIGPLCFPSLPWVDP"
BASE COUNT         1322 a         3216 c         3271 g         1315 t
ORIGIN      
        1 gaggttgacg agggagcgga ggatccgcgt cttggcctgg ccgcgcaggc cgaggaggac
       61 gacgtcgtgc cgggcgagga cggcgttgac gagggcgggg acgacggtcc gctcgtaccc
      121 gaggatcccc gggaaggggt tctccttccg cgcgagcatc gcgaggaggt tctcgcgcat
      181 ctcgtctcgc acggagcggt gccgatagcc ggcctcgacg agctccccga gggtcgtggg
      241 tcgatatgtc gtctccaccg tccgctcctc cgttcccgga tcgtacgact caggccggct
      301 tgcggatccc gaacttctcg agccggtact ggagggtccg gtacgtcagc ccgaggaggc
      361 gggcggcgcg ggcgatgacc caggcggacc gctccatcgc ctggacgatc agctgccgct
      421 cgagctcctc gatgtcgatc ccctccggcg ggatctcgaa gccgtagggg cgcgcggcgg
      481 gcgcggtctt cagccggatc tcgagcggca ggtcggcggg ccgcacggtc gtcccttcgc
      541 agaggaggac ggccctctcg acgacgctct cgagctgccg gatgttcccg ggccaggggt
      601 gccggacgag caggtcgagc gccgccgggt cgatgtcggc aacggcggtc ccgtgggtcc
      661 cgttcgcctt gtcgaggaac ctgtggacga gcgcggggat gtcgtcggac cgctcccgga
      721 gcggggggag gacgatgggg atgacgttga gccggtagaa gaggtcctcg cggaactgtc
      781 ccgccttcat catcgactcg agctcgcggt tcgtggcggc gacgacgcgg acgtcgatcg
      841 cgatcgtctc gttccccccg acgcgacgga tctcgcgctc ctggagggcg cggaggatct
      901 tcccctgaag cggaagcgag aggtctccga tctcgtcgag gaagagcgtc gagccggagg
      961 cggcctcgaa gagcccggtc ttgcgcgcgt cggcgccggt gaaagagccc ctctcgtggc
     1021 cgaagagctc gctctcgagg aggctctcgg ggatggcggc gcagttgatc gcctggaacg
     1081 cccgcgccgc cctcgccgag cgcgagtgga tcgcgcgggc gacgagctcc ttgcccgtgc
     1141 ccgactcgcc ccgcacgagg acggtcgact gcgtgggggc gatcttctcg acgagccgga
     1201 agacctcgcg catcgccgag ctctcgccca ccatcgcctc gagcccgtct cccttgagac
     1261 ggcgctcgag ccgcagcgcc ctttcggcga gccggtggct ctcgagggcg cgctccacgc
     1321 ggaagaggag ctcgtcggcg acgacgggct tgctcatgta gtcgaacgcg ccgaggcggt
     1381 gcgcctctcg ggcccgttcg accgagccgt gggccgtgat gaggatgacg cggggcggga
     1441 gcggtccctc cacgagggcc cggacgaggt cgaggccgga gagccccggc atcttgaggt
     1501 cggtcaggac gacctcgggg tgcgtctccg cggcgagagc gagggcctcg cgaccgtccg
     1561 cggcggcggt cacgcggtac ccctcgtcct cgaggatcat ccggagggac tcgcgctggt
     1621 gcttctcgtc gtcgacgacg aggagggtcg cgcggggcgt gcgttcgttc acccggggag
     1681 gttactccga aggggcggcg acgacgggga gggagatccg gaagagggcg ccgtgccccg
     1741 attccctgtt cccggcctcg acccttcccc cgtgctcctc gacgatccgt ttcaccatcg
     1801 cgagccccag cccgaccccc ccttccttcg ttgagaacca gggctcgaag agcctcggga
     1861 ggtgggcggc gtcgatcccc ggtccttcgt cctcgagcgt gaggacgacg cgcggccctt
     1921 cgtcgacggt gccgtccgcc aggcgcgccg cgacccgaag agtcccgcct tccttctcca
     1981 tcgtctggat cccgttctgg gcgaggttcc agatcaccga cttcaggagc gagccgtccg
     2041 ccgcgacggg gggcaggccg gcggggaggt cggtcgagaa ccgcacgcgc acctgcccgg
     2101 ccgtctcggc caggtcggcg agcgtgtccc ggacgagggc cgccgcgtcg accggcagga
     2161 tcgagagcgt gagcgggcgg ccgaacgcga ggaagttgtt gatgagcgcg gccagccggt
     2221 cgatctcgcc ccggagggcg tcgatccgct ccgcctgctc ggcggcggcg gcctgctcgg
     2281 ccgggcggtg gcgtctcctg aggacgtcga ggccgaggga gagggcgttc agcgggttct
     2341 tgatctcgtg cgcgaggccg gccgcgaggt ggccgatctc ggcccgcttc tcggcggcgg
     2401 cgaggcgcgc gaggagctcc tggcgctccc ggagcgcggc ggtcatccgg ttgaaggcga
     2461 tcgcgagctg cccgatctcg tcggcccggt cgaccgggac gacgacgtcg agctggccgt
     2521 ccgcgacctg ccgcgccgcc tcggcgaccg cctccacggg gcgagtcgcg ttccgcgtga
     2581 cgacgaggat gacgacgagg ccgacgccga agacgccgag cagggcgtag aggcctctcc
     2641 ggaagttgtc ctcgagctcg acccggatgt tctcgagcga gtacttgatc gtcacctgcc
     2701 cgagatagcg ccgctcgatg acgagcggga cgacgatctc gccggagccc gccccctcgc
     2761 cgacgacccc ttcgatctcg atgtcctggg ggccggaccg cggccgcgcg cgccggcgcg
     2821 cggtcgtggc agtcccggcg ggcgctccgt cgggcaggag ctcgcctccc tgcttgatcc
     2881 ggatctcgcg gacccccttc tgcttcagcc gctcgcggaa ctcctcgagc gcctccgggt
     2941 cgcgggagcc ccccgggccg atccgcgtcg ccgccgcctg gatcgcggtg gcgagcgtct
     3001 tgtaggaggc atccgcctgc gccagcatct ggttctgcga ccgcaggtag aggaccgtct
     3061 gcgcggcggc ggcggccacc aggattccgg tgagggcgag gccgaggcgg agcgtgaggc
     3121 tcggaccgcg gaagggattc cggagcggcg acatcacccc gcgattctcc gccacgtccg
     3181 gagatcggcg aagggcgccc gccgcccgag cgcggctcgt tcggcccggg cgcgcttacg
     3241 cggtggactc gggccgggcg cttcgggtac aaggcgcgct atcgcacccc gacgaggagg
     3301 tccgccatga gcgacggaac gcccgagaag gccctcgaca cccttctcct cgagcaccgc
     3361 accttcgacc cgtccccgga gttccggcgg tcggccgtcg cgagggaccc gggcgtctac
     3421 gcgcaggcgg cggccgaccc cgagtcgttc tgggcctcct tcgcgcgcga gctggagtgg
     3481 atccgcccgt acgaccgggt cctcgagtgg aaggcgcccg acgcgaggtg gttcgtcgga
     3541 ggccgcctca acgtctccgc gaactgcctc gaccgccacc tccactccgc gcggcgccac
     3601 aaggccgcgc tcgtctggga aggcgagccc ggcgagtcgc gcatctacac ctaccagcag
     3661 ctccaccacc acgtctgcaa gttcgcgaac gtcctgaaga ggctcggcgt cgggaagggg
     3721 gaccgggtct gcatctacct cccgatggtc ccggaggcgg tgatcgcgat gctcgcctgc
     3781 gcgcggatcg gcgccgtcca ctccgtcgtc ttcggcggct tctcggccga cgcgttgaag
     3841 gaccggatcc aggacgccgg ggccaagctc ctcgtgacgg ccgacggagg gtggcgccgc
     3901 gggcagatcg tccagctcaa gaagatggcc gacgaggcgc tgaaggagtg cccgacggtc
     3961 caggccgtcg tcctcgtcaa gcggggggac ttcccctgcc acgtgaagga ggggcgcgac
     4021 cactggtggc accggctcat ggacgaggtg cggggagact gcccgtacga gtcgatggac
     4081 tccgaggcgc cgctcttcat cctctacacg tcggggacga ccgggaagcc gaaggggatc
     4141 ctccacacga cggccggcta cctcaccggc gtctacggca cgacgaagtg ggtcttcgac
     4201 ctgaaggacg aggacctctt ctggtgtacg gccgacgtcg ggtgggtcac cggccacagc
     4261 tacgtcgtct acgggccgct cgccgcgggc gccaccgtcc tgatgtacga gggggctccc
     4321 gactggcccg acaaggaccg cttctggcag atgatcgaga agtacggcgt caccgtcttc
     4381 tacacggcgc cgacggcgat ccgcgcgttc atgaagtggg ggacgcacta cccggagaag
     4441 tgggacctct cctcgctccg gctcctcggc tccgtcggcg agccgatcaa ccccgaggcg
     4501 tggatgtggt accaggagca catcgggcgc aggcgctgcc cgatcgtcga cacctggtgg
     4561 cagacggaga cggggcacat catgatcacg cccctccccg gcctgacccg gacgaagccc
     4621 ggctcggcga cgatgagctt cccgggcgtc gacgcctgcg tcctgaacga gaagggcgag
     4681 gaggtgacgg aagggggcgg ctacgtcgcc gtccgcaagc cgtggcccgg gatgctgcgc
     4741 gggatctggg ggaaccgcga gcgcttcgtg aaggcctact ggtcgaagtg ggacggggtc
     4801 tacttccccg gggacggggt gaggaaggac gaggacggct acttctggtt cctgggccgc
     4861 gtcgacgacg tcctgaacgt cgccgggcac cggatcggga ccgccgaggt ggagagcgcg
     4921 ctcgtggact acccgctcgt cgccgaggcg gccgtcgtcg gaatcccgca cgacctcaag
     4981 gggacggcga tcgccgcgtt cgtcaccctg aagtcggggc accacggctc cgacgagctc
     5041 gtgcaggacc tgaaggagca cgtcatcgag aagatcggcg ccatcgcgcg gcccgacaag
     5101 atcctcttct cggccgacct cccgaagacc cgctccggaa agatcatgcg gcgcctcctt
     5161 cgcgacatcg ccgagggacg cgtcctcgga gacacgacga cgctcgccga cccgaacgtc
     5221 gtgaagcagc tccgcgacca gtacgagggc aacgagagct gaggtcgtga ggaggggccg
     5281 tctacgggat ccagatcccc gagacgagct cggcgaccgt tcgccagctg aaccagaggc
     5341 cgaagccgag gaggaggacc ccggcggcga aggacgactt gtggatccac tccccggagg
     5401 ggtggttccg cagccgctcc acgagctcga cgaggatcgc gaaccaggcg aagacgccga
     5461 gccccacgcc gacggcgaag acgcctgcag gaaggaggcc ggtcggcagg atccggtgtc
     5521 ccacgaggaa cgagatcagg agcgtccagt tgacgaagag ggcggggttc gccgcgccct
     5581 ggaggacgcc gacgaggagc ggaaagcgga agcccgagac ctccttcggc ggggccgtca
     5641 ggccggggcg cgccgttctc tcggcgtccc gcttgcggtc gaggacgagg atcttcagcc
     5701 cgctcgccag gaggaagagg gcgaggccga ggcgggtcca ggggttcgcg acgactttct
     5761 cgagcgccca gccgaagccc atcgccacga agccgcagat caccgtgtcg acgagcgccg
     5821 cgccgagggc gacccggagc gcctcccgcg tctccgcgcg cgacgccttc cggatgacgt
     5881 tcacgttgac gggccccggc ggaattgcgc cgaagaggcc gacggcgaag cccacgagga
     5941 gggccatcgg cagctccttc acgcccgcct gctccgccgc gacatgaggg cggaatcgtc
     6001 tcacgtctgt tgcgacggtt cgagcggaag gctaatgtga acgcatgaag gcctgcgtcc
     6061 cattcctcgc tctcgcgctc gcgctcccgg ccgcggcgca ggaggagcgg ctggagttcg
     6121 acccccaggt cgggatgacg gggacccgcg tcaccgtcac gagcccgctc ccgaaggggg
     6181 cggtcgtccg attcggcacg cgcgtcgttc cgctcctcca ggagccgggc cgccggccga
     6241 gcttcgtcgt ccccgagggc gcgaagagcg ccttcatcga agcctctctc gacgggaagc
     6301 cgttctcgcg gagcgccgtc ccgttcgtcg tcgcgggtgt gtcgctcgtc ggcgttcaga
     6361 agctcgtcgg cctccgcgag gcgatcgacg tcttcggcta ctccgacccg accccggagg
     6421 gcggcggcgc gccggagcag agggcgaaga agatcctctc cttcggcgac ggcgacctca
     6481 tcacgatcgg cgaggcgccg gcccccgcgc cccccctgtg ggtcatcggg aacgacgcgg
     6541 cctcggcggc aatgaggggg atgggcccgc ccgggttcct cctgacggcc cggccgccga
     6601 cgcgccggat catcgtcatc cctccgcctc ctcccgtccc gacgccggta ccgactccga
     6661 cgccctcgcc ctcgccgggc tgaccgcgcc tcgcgccggg aaccagaagc gctccccgtg
     6721 ggtcacacgg ggcatggagg gacctccgtg acggcccgga tcctcgcgtt tcaccccggc
     6781 cccggggcgc atcaccgtgg gagaatcccc gccgtggttc tccccgtgaa cggccccagc
     6841 gaagcccggg actggcccgg agacgagaag gccgtcgccc gattcctggg cggcgacccg
     6901 accggcttcg agcagatcgt ccgtcactac tccggaatgg tcttctccct cgccgcgcgg
     6961 ctcgtcgggc cgtgggaagc cgaggaggtc gtccaggaga cgttcctccg cgcctggcgc
     7021 ggcctcgaga cgttccgcgg agactcgtcc ctcaagacgt ggctctacac gatcgccctg
     7081 aaccgtgcga aggcgcgcca ggggacgctg gcccggatgc gaaaggtctt cgcctcccgc
     7141 cgggccgccg aggaggagga tcgaccctgg ccgggcgaag aggctccgga cccctccgcg
     7201 tcccccgagg agcagaccct cgcgctcgag cagcggaccc ggcttcgcag ggcgatccgg
     7261 aacctccccg aggagttccg gcacgccgtc gtcctgcgcg acctggaagg tctctcctac
     7321 gacgagatcg cacgcgtcct cggcgtcccg atcgggacgg tgaggagccg gatcgcccgc
     7381 ggccgggcgg ccctcctgga ggagctttcg tgagcgcgaa cccccagccc cgccctctcg
     7441 tgacggggga cctcgacctc ctgatctccc gctccctcga cggagacctt ccgggcgagg
     7501 aggagcgcga cctgaagagc ctccttgcgg ccgacgcggc ggcgcgcgcc cgcttcgacg
     7561 cgatggcccg cctcgtcgac cggctggaag agctgcccga agcggagact cccttcgccc
     7621 tcgcgacccg ggtcggatcg caggtcgagg ccgacacgca gggcttcgcc gccacgctcc
     7681 acaagtacgg cttctacttc cggcccgcca cgttcggcgt catcgcggcg gggctcgtcg
     7741 tcgtggccgt catcgcgacc ctgcgcaacc cggcgaagcc cgtggcgacc gtggcggagg
     7801 tgaaagcctt gcccgctccg gcggccggcg aagatcgcat gaccgtctac ctcgcgacga
     7861 cggagaagcc gaaggaagag gcggcggctc ccgccgatcc cgcccctgcg gcttccggcg
     7921 tccctgccga ggcgaagggc gtcggccgcc ccgagccggt cctcgccgcg aaggccgaga
     7981 gtcccgtcgg acgggaccag gccggcttcg ctccggaacg cgacgcggct gcgaacgacc
     8041 ggctcgcttc ggcgacgtcc gcttcggaag gccgagccgc cgccgggagc gccgaggtcg
     8101 tccgtcagga gaccgaggcc gcacccgcgc gtctgcgcgc cgcagcgccg gccccggctc
     8161 cgccgagcgc ggagcgcccg tcgcccaggc tggacctcgt cgggagttcg tccgccggcc
     8221 tgcgcctggt cgccccggcc cgcctcgacg gcctcccggg gccgttcgac ggcagctatc
     8281 gcctcgagct caacgcctcc gggcgcgtct ccgaggtcgt ccgcctcttc gggggctccg
     8341 gaacggagcc cccggcgctt ccggaccggc tgcggactct ttcgttcgcg tcctcgaacg
     8401 gagcccccct cgccggcccg gtcgacgtga gggtccggct cccatagtcc ggcccgaatt
     8461 actcgacaga tctattcgtg taagtcattt attttgaaat atttgtgtgt cttccggtag
     8521 gggtggccaa gcacccctcc ctcgggcaga atgggctcgc cacgggggaa cgatcgtgaa
     8581 gaggacgcag gcggctcggg ttcggaaggc cctggatttc gtgaagaagc ggttgaagct
     8641 cgacctcgag ttcgtcgtcg agccgggaga ggtgcacgaa cagttcggcc tcctcgacat
     8701 cgaacgctcc atgcgcggga ccgaggaccg gaaggtctgg gtcgtttcgt tcgaccccga
     8761 gctcgtctcg aaggagtcgc tcgctgcgct ccgccgccac gccttccacg aggtgctcca
     8821 cgccctcacc tggccgctct tcgacgaggc cgaggcggcg atccgaagcg ttcccgactc
     8881 gaggcttcgc aaggagctga tggatcgctc catcgacgcg cgcgagaacg tcgtctacga
     8941 gctcgagcgg aagatcggcc ccctctgctt cccgtcgctc ccctgggtcg acccctgatc
     9001 gcttgaccgt ccgggggccg gcttctagaa tgcgcgtccc ttgcggcgtc ttgccgcgct
     9061 ctttggcagg gaacgggccg aaatagctca gtcggtagag caactgattc gtaatcagta
     9121 ggtc
//
LOCUS       DAOTEZ010000153         9102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5415_length_9102_cov_5.744998, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000153 DAOTEZ010000000
VERSION     DAOTEZ010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9102
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5415_length_9102_cov_5.744998"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="P5164_09500"
     CDS             <1..232
                     /locus_tag="P5164_09500"
                     /inference="COORDINATES: protein motif:HMM:NF013821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HRY44167.1"
                     /translation="CVTNIGRRPTVYEDYATTIESYILDFSSDVYGEPIRLSFHERLR
                     DEQVFPSMLELTAQIRRDVEATRLWFLARGRG"
     gene            235..1101
                     /locus_tag="P5164_09505"
     CDS             235..1101
                     /locus_tag="P5164_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44168.1"
                     /translation="MDTDLPRVVLALASLALVRRARRPRGAALAGAAGAAAAAGATLP
                     ALPSPWLFGGVLLAAALVLTALRADLHSPLVSEAAAFTLALAAGAGAVTALALAGTGP
                     QGAAAGAGAVFAAVGVWGAEGSRACAEAGWRRGVAWGSGAILAGAVAAGIVALAVRLP
                     ARLALLAPAAATLAALLAWLPALLLERARVRSELGEEARLGILPEEDLSVLGSPWRRR
                     REARLGRADERREYVRSALLLAVARQQQRRRTGEAVRLRQLEVLAFRTRVRRAVEGRK
                     ARFAGLGGEPEP"
     gene            1152..2228
                     /locus_tag="P5164_09510"
     CDS             1152..2228
                     /locus_tag="P5164_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44169.1"
                     /translation="MRVLRPLQALLPSLLLAAAPAPAETPAEGGKPRFVLGATLSDLE
                     PVHERELRDGELLFAFLSAEVERDGWGSRLELRAREGRFRDYFPGPVWLEEGYAWVET
                     PLGELRAGKVPSVVGLADETFGGTLFSLNGVNRNPDWGVQIVGSRPLGWHSLDWAARW
                     AGSNDRVAWEEDGKGVESDPAARLRDGLSGRVSFLFNKGLWTVRPGLSGATARIVASD
                     GRPEYRRSDASADLSATLGPISLQLQGLLRRADEPVAGEFRPEAERDGRALLVGAGVE
                     FPESTFRYVYSEWRYYGAASNERIHQPAAVWKGRKGIEAIVEYTARRIRIGETVRTFD
                     AFRLGLVLRFPFGDSPGRSRGAKG"
     gene            2233..3525
                     /locus_tag="P5164_09515"
     CDS             2233..3525
                     /locus_tag="P5164_09515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY44170.1"
                     /translation="MKKALVGAAIVAAVVVAAVLLLSKDKKSGPKFRKEKVTKGSVVA
                     TVTATGTLSAVTTVKVGSQVSGIIARLHADFNSEVRRGQLLAELDPTPFQATVEQRRA
                     DLERARVELRNAELVLARAKKLLEAQLQAQSEYDTAKANRDGAAASVAQSSASLKQAE
                     TNLAYTRILSPIDGVVVDRQYDIGQTVAASFQAPVLFTIAQDLTKMQVLTNIDEADIG
                     RVKEGQEATFTVDAFAERTFTGRVSQIRLSPQTVQNVVTYPVLLDVSNPELRLRPGMT
                     ANVLVPVDRRDDVLRVPNAALRFKPDPADLEGGGKAKPDAAAPSGAARAEGAGNGTAG
                     APAGGRPGRGEGGRPGGGAGRGGAGRSGPSRSGEVYVELPTGKLRVVRAKTTLTDGNL
                     TAVETDDLKEGDEVVLGLATARAGSGAGGGAPRGMGRM"
     gene            3588..4265
                     /locus_tag="P5164_09520"
     CDS             3588..4265
                     /locus_tag="P5164_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY44171.1"
                     /translation="MGDSEVRALDGVTLDVAPGEFLAVMGPSGSGKSTFMNILGCLDR
                     PTSGTYVLEGTDVSTLDRNERAGIRNDKIGFVFQSFNLLSRTSALENVELPLLYSRKH
                     RLSDAGQLEKARGCLAKVGLAERWDHTPAQLSGGQQQRVAVARALVNDPAILLADEPT
                     GNLDSRTSDEIMGIFQELNEEGKTVVLITHEHDIAEYARRVVAFRDGRIVSDDVVKER
                     RSAKGTR"
     gene            4262..5488
                     /locus_tag="P5164_09525"
     CDS             4262..5488
                     /locus_tag="P5164_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY44172.1"
                     /translation="MTKTTSILKVAFRAMGRNKMRSLLTALGIIIGVACVVATIGIGE
                     GARVQTEQQLQSLGTNFLMIHPGTVTSSGARSGWGSNSKLSEEDVAAIRAEVPTVSYV
                     SASIRSVAQVVYGNQNWSTSIQGGEVDWPAIRSWNVAQGQFFTDQDNRAAAKVCVLGQ
                     TVATTLFGTEDPVGKTIRIKNIPFRVVGVLEPKGGSMMGQDQDDTVVAPYQTVRKKIM
                     GTTAVGMIMAAVSTPELVDRAQEEITALLRQRHRINRAAGQEDDFMIRSQTEMLARAE
                     EQSKTMAILLWSIAGVSLLVGGIGIMNIMLVSVTERTREIGVRMAIGAKGSDIRAQFL
                     VEAVVLAIAGGVLGIALGVGIQEAVAKLAGWPVAVTTSSVSLAFVFSAVIGVTFGFYP
                     ALKASRLDPIEALRYE"
     gene            complement(5710..6369)
                     /locus_tag="P5164_09530"
     CDS             complement(5710..6369)
                     /locus_tag="P5164_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015277947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY44173.1"
                     /translation="MGLVLVETFPVAPLGCNCAIVADPVSKAAIVLDPGGEPDRVLAS
                     LARHGLNAVALVHTHAHLDHCLSSARLSKETGAPILMHEGDLPLYGDLGRWGRMFGLT
                     PEAPGPVARTLGHGERVACGAGALEVLHTPGHTPGSVCFRLEGDSPLLFSGDTLFRRS
                     VGRTDFPGGSWDDLVSSIETRLLTLPGDLVVLPGHGEETTIGEESRLNPFLGDRSRPV
                     A"
     gene            6423..7475
                     /locus_tag="P5164_09535"
     CDS             6423..7475
                     /locus_tag="P5164_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HRY44174.1"
                     /translation="MSAAPISDFRSDTVTKPTPAMRRAMAEAEVGDDVYGEDPTIARL
                     EARTAELLGFPAALFVPTGSMGNQIALRVHARPGTEVIVEARSHVFHYEMGAMAALSG
                     LLPRPVFGPGGRMPEAEAEAWVRPESVYYLPRTSLVCLENTHNFAGGTVLPRAAVEEV
                     LALARRKGLAVHVDGARLWNAAAALRVSEASLVAGVDSVMVCFSKGLRAPVGSAVAGS
                     EAFVAEARRARKLFGGGMRQAGVLAAAALVALEEERGRLAEDHARLARLARELAGVRG
                     VSVDPESFPTNILIVDLDARVWGSANDALGKLRTRGILAGAAGPASVRLVTHADVGDA
                     DVDRCVAAFRESAGVS"
     gene            complement(7501..7884)
                     /locus_tag="P5164_09540"
     CDS             complement(7501..7884)
                     /locus_tag="P5164_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRY44175.1"
                     /translation="MKTIAVENAPKAIGPYAQGIVAGGFLFSAGQVHLDPATMKLVSG
                     TIAEETNQVFDNLEAVLAGAGCTLADVVKTTVFLTDMGSFAEMNGAYAARFGDHRPAR
                     STVQVGALPGNGRVEIELIAKLPGA"
     gene            complement(7890..8408)
                     /gene="hpt"
                     /locus_tag="P5164_09545"
     CDS             complement(7890..8408)
                     /gene="hpt"
                     /locus_tag="P5164_09545"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRY44176.1"
                     /translation="MIDRILISEQQISARLDELAREIDSAYAGREILAVGVLKGSVFF
                     LTDLLKRLSTPVAVDFLQVRSYSGTASTGNFELVRDLSSDIAGRDVLIFEDVVDTGLT
                     LRKLLGLLGTRGPRSVRIAALLKKDLPENADLPVDFLGFTIGPEFVVGYGLDLDEKYR
                     NLPYVAIWDPSK"
     gene            8450..>9102
                     /locus_tag="P5164_09550"
     CDS             8450..>9102
                     /locus_tag="P5164_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44177.1"
                     /translation="MSTSIVDLKTIHDAYTRLTEKFKTFWTFHQFLQGVHKTFFGDAP
                     GYNVDFQGLYDEIRAVTTVMNVEPPPVVIDRIHQLDGRLDVVYRALSADDEKIAASWV
                     RRFFEKVRTEDEKLLLAILRFYFHARQVSPEALDKMDFLLTLVGARRSLDDGRYLPRF
                     PQELTKLFASLLGLVRRNPAPAAELTASVKALGLLRKDIDSCERFEDLTEKKILDSLR
                     "
BASE COUNT         1317 a         3163 c         3367 g         1255 t
ORIGIN      
        1 ctgcgtcacg aacatcggcc gccggccgac cgtctacgag gactacgcga cgacgatcga
       61 gtcgtacatc ctcgacttct cctcggacgt gtacggcgag ccgatccgcc tctcgttcca
      121 cgagcggctc cgagacgagc aggtctttcc gtcgatgctc gagctgacgg cccagatccg
      181 ccgcgacgtc gaggcgaccc ggctctggtt cctcgcgcgc gggaggggat gagcgtggac
      241 accgacctcc cgcgcgtcgt cctcgccctc gcttctctcg ccctcgtccg cagggcgcgc
      301 aggccgcgcg gcgcggcgct ggccggcgcc gccggggccg cggcggcggc cggggcgacg
      361 ctgcccgcgc tcccttcccc gtggctcttc gggggcgtcc tgctggccgc cgcgctcgtc
      421 ctgacagcgc tccgggcgga cctccattcg cctctcgtgt cggaggcggc agccttcacg
      481 ctcgcgctcg cggcgggggc aggtgcggtc acggccctcg cgctcgcggg caccgggccg
      541 cagggcgcgg cggccggagc aggcgcggtc ttcgcggcgg tcggcgtctg gggcgccgag
      601 gggtcccgcg cctgcgccga ggccggctgg aggcgtggcg tggcgtgggg gagcggcgcg
      661 atcctcgccg gggccgtcgc agcggggatc gtggcgctcg cggtccgcct tccggcacgg
      721 ctcgcgctcc tcgcgcccgc cgcggcgacc ctcgccgcgc tcctggcctg gctcccggct
      781 ctcctcctgg agcgggcccg cgttcgcagc gagctcggcg aggaggcccg cctcgggatc
      841 ctccccgagg aggacctctc cgtcctcggc agcccgtggc ggaggcggcg ggaggcccga
      901 ctcgggcgcg cggacgagcg ccgcgagtac gtccggagcg cgctcctcct cgcggtcgcg
      961 cgtcagcagc agcgccggcg aaccggcgag gcggtccgtc tccggcagct cgaggtcctc
     1021 gccttccgga cccgggtgcg tcgcgcggtg gaggggcgca aggcccgctt cgccgggctc
     1081 ggcggcgaac ccgagccctg acggcgcacg ccgcgcggcc cggcgacggc cgcgccgtat
     1141 catccggagg cttgcgagtc ctccggcccc ttcaggcgct cctcccgtcg ctcctgctcg
     1201 ccgctgcgcc cgctccggcc gaaacgccgg cggagggcgg gaagccccgc ttcgtcctcg
     1261 gggcgaccct ttcggacctc gagccggtgc acgagaggga gctgcgcgac ggcgagctcc
     1321 tgttcgcctt cctctcggcc gaggtcgagc gggacggctg gggctcccgc ctggagctgc
     1381 gggcgcgcga aggacggttc cgcgactact tcccgggccc ggtgtggctc gaagagggct
     1441 acgcctgggt cgaaacgccg ctcggcgagc tccgcgcggg aaaggtcccg agcgtcgtcg
     1501 gcctggccga cgagacgttc ggcgggaccc ttttctcgct caatggagtc aaccggaacc
     1561 ccgactgggg cgtccagatc gtcgggagcc ggccgctcgg ctggcactcc ctcgactggg
     1621 cggcgcgctg ggccgggagc aacgaccggg tcgcctggga agaggacgga aagggcgtcg
     1681 aatcggatcc cgcggcacgg cttcgcgacg gcctctcggg ccgggtctcc ttcctcttca
     1741 acaaggggct ctggacggtg cgaccgggcc tgtccggcgc gacggcgagg atcgtcgcgt
     1801 ccgacgggag gccggagtac cggcggagcg acgcctcggc cgacctctcg gcgacgctcg
     1861 ggccgatctc cctccagctc cagggtctcc tgcgtcgggc ggacgaaccg gtcgcggggg
     1921 agttccggcc ggaggccgag agggacggcc gcgcgcttct cgtcggggcc ggcgtcgagt
     1981 tcccggagtc gaccttccgc tacgtctaca gcgagtggcg ctactacggc gccgcgtcga
     2041 acgagcgaat ccaccagccc gcggccgtct ggaagggccg gaagggaatc gaggcgatcg
     2101 tcgagtacac cgcgcggcga atccggatcg gagagaccgt ccggacgttc gacgcgttcc
     2161 gtctcggcct ggtcctccgc ttcccgttcg gggactcgcc gggccgaagc cgcggcgcga
     2221 aaggctgaat cgatgaagaa ggctctggtc ggagcggcga tcgtcgccgc cgtcgtggtg
     2281 gcggccgtcc tcctcctttc caaggacaag aagagcgggc cgaagttccg gaaggagaag
     2341 gtgacgaagg gctccgtcgt cgcgacggtc accgcgaccg ggacgctctc ggccgtcacc
     2401 accgtcaagg tcggcagcca ggtctcgggg atcatcgcga ggctccacgc cgacttcaac
     2461 tccgaggtcc ggcggggcca gctcctcgcc gagctcgacc cgacgccgtt ccaggcgacg
     2521 gtggagcagc gccgggccga cctggagcgc gcgagggtcg agctgcgcaa cgccgagctc
     2581 gtcctggccc gggcgaagaa gctcctcgag gcgcagctcc aggcgcagtc ggaatacgac
     2641 acggccaagg cgaaccggga cggggccgcc gcgtccgtgg cccagtcgtc ggcgagcctg
     2701 aagcaggccg agacgaacct cgcctacacg aggatccttt cgccgatcga cggcgtcgtc
     2761 gtggaccggc agtacgacat cggccagacc gtcgccgcct ccttccaggc gccggtgctc
     2821 ttcacgatcg cgcaggacct gacgaagatg caggtcctga cgaacatcga cgaggccgac
     2881 atcggccgcg tgaaggaagg gcaggaggcg acgttcaccg tcgacgcctt cgccgagcgg
     2941 acgttcacgg gacgggtctc gcagatccgc ctctctcccc agacggtcca gaacgtcgtc
     3001 acctatccgg tcctcctcga cgtgtcgaac ccggagctgc ggctgcggcc cggaatgacg
     3061 gccaacgtcc tcgtcccggt cgaccggcgg gacgacgtcc tgcgcgtgcc gaacgcggcg
     3121 cttcgcttca agcccgaccc ggccgacctc gagggcggcg gaaaggcgaa gcccgacgcg
     3181 gccgcgccgt cgggcgcggc acgtgcggaa ggagccggaa acggcacggc cggggctccg
     3241 gccggcgggc gaccgggccg aggcgaaggg ggccggccgg gcggcggcgc gggacggggc
     3301 ggggccggcc gttccgggcc ctcccgctcg ggagaggtct acgtcgagct cccgaccggg
     3361 aagctccgcg tcgtccgcgc aaagacgacg ctgaccgacg ggaacctgac ggccgtcgag
     3421 acggacgacc tgaaggaggg ggacgaggtc gtcctcggcc tcgcaacggc ccgggcgggg
     3481 agcggcgcgg gcggtggcgc gccgcgcggc atggggcgga tgtaggcgtg gccgcgagct
     3541 cggccgagcc ggtcatccgg atacgcgacc tgacgcgcgt ctaccggatg ggggactccg
     3601 aggtgcgggc cctcgacggc gtcacgctcg acgtcgctcc cggcgagttc ctcgccgtca
     3661 tgggcccttc gggatccggc aagtccacgt tcatgaacat cctcgggtgc ctcgaccgac
     3721 cgacttccgg cacctacgtc ctcgagggga ccgacgtctc gacgctcgac cggaacgaga
     3781 gggccgggat ccggaacgac aagatcggct tcgtcttcca gagcttcaac ctcctctcga
     3841 ggacctcggc gctcgagaac gtcgagctgc cgctcctcta ctcccgcaag caccggctct
     3901 cggacgccgg gcagctcgag aaggcccggg gctgcctcgc gaaggtcggc ctcgcggaac
     3961 gctgggacca cacgcccgcg cagctctccg gcggccagca gcagcgcgtc gccgtggccc
     4021 gggccctcgt gaacgacccg gcgatcctcc tcgccgacga gccgacgggg aacctcgact
     4081 cgaggacctc cgacgagatc atggggatct tccaggagct gaacgaggag gggaagacgg
     4141 tcgtcctgat cacccacgag cacgacatcg ccgagtacgc gcggcgggtc gtggcgttcc
     4201 gcgacggccg catcgtctcc gacgacgtgg tgaaggagcg tcgatccgcg aaggggacgc
     4261 gatgacgaag acgacgagca tcctgaaggt cgcgttccgc gccatggggc ggaacaagat
     4321 gcgctcgctc ctgacggcgc tggggatcat catcggcgtc gcctgcgtcg tggcgacgat
     4381 cgggatcgga gaaggggcga gggtccagac cgagcagcag ctccagtcgc tcgggacgaa
     4441 cttcctgatg atccatcccg ggacggtcac ctcctcggga gcgcggtcgg gctgggggtc
     4501 gaactcgaag ctgtccgagg aggacgtcgc ggcgatccgc gcggaggtcc cgaccgtctc
     4561 ctacgtctcc gcgtcgatcc gctcggtcgc gcaggtcgtg tacgggaacc agaactggtc
     4621 gacgtcgatc caggggggcg aggtcgactg gccggcgatc cgctcctgga acgtcgcgca
     4681 ggggcagttc ttcaccgacc aggacaaccg ggcggcagcg aaggtctgcg tcctcgggca
     4741 gaccgtcgcg acgaccctct tcggcaccga ggatcccgtc gggaagacga tccggatcaa
     4801 gaacatcccg ttccgcgtcg tcggcgtcct cgagccgaag gggggctcga tgatggggca
     4861 ggaccaggac gacacggtcg tcgcccccta ccagacggtc cggaagaaga tcatggggac
     4921 gacggcggtc gggatgatca tggcggccgt ctccacgccc gagctcgtcg accgggccca
     4981 ggaggagatc acggcgctcc tccggcagcg ccaccggatc aaccgcgcgg cgggccagga
     5041 ggacgacttc atgatccgct cccagaccga gatgctcgcg cgcgccgagg agcagtcgaa
     5101 gacgatggcg atcctcctct ggtcgatcgc gggcgtctcg ctcctcgtgg gcgggatcgg
     5161 gatcatgaac atcatgctcg tctccgtgac ggagcggacg cgcgagatcg gcgtgcgaat
     5221 ggcgatcggc gcgaagggat ccgacatccg cgcgcagttc ctcgtcgagg ccgtcgtcct
     5281 cgcgatcgcc gggggcgtcc tcgggatcgc gctcggcgtc ggcatccagg aagccgtggc
     5341 gaagctcgcc ggctggcccg tcgccgtcac gaccagctcc gtgtcgctcg ccttcgtctt
     5401 ctccgccgtc atcggcgtca ccttcggctt ctacccggcg ctcaaagcct cgagactgga
     5461 tccgatcgag gcgctgcggt acgagtgagc tgaagcgggg ggtccggggg gcgcgcggag
     5521 ccaagccccc cgggagcggc gagagactgg atccgatcga ggcgctgcgg tacgagtgag
     5581 ctgaagaggg gggccggggg gcgcgcgcaa cgcgtcttct cgcgtcccac cccgcgcccc
     5641 gcgttcatcc cgcccgcgcg aagcgcgcac cgcgaagggc ggtgggtggg gggcgcgggg
     5701 tgggggcctt caggccacgg gccgggagcg gtcgccgagg aacgggttca gcctcgactc
     5761 ctcgccgatc gtcgtctcct cgccgtgacc cggcaggacg acgaggtcgc cgggaagcgt
     5821 cagaagacgg gtctcgatgg acgagacgag gtcgtcccag gagccgccgg ggaaatcggt
     5881 ccggccgacg gagcgccgga agagggtgtc gcccgagaag aggagggggc tgtcgccctc
     5941 cagccggaag cagacgctcc cgggagtgtg accaggcgtg tgaaggacct cgagagcccc
     6001 ggcgccgcag gcgacgcgct ccccgtggcc gagagtcctc gcgacgggac cgggagcctc
     6061 gggagtcagg ccgaacatcc gtccccagcg gccgaggtcg ccgtagagcg gaaggtctcc
     6121 ttcgtgcatc aggatcggcg cgcccgtctc cttcgacagc ctcgccgagg agaggcagtg
     6181 gtcgaggtgg gcgtgcgtgt gaacgagcgc gacggcgttc aggccgtggc gggccagcga
     6241 ggccaggacg cggtccggct caccgcccgg atcgaggacg atcgccgcct tcgacacggg
     6301 gtcggccacg atcgcgcagt tgcagccgag gggggcgacg gggaaggtct ccacgaggac
     6361 gaggcccatg gcggccgagt ctgccacacg gtcccggggc gcctcggcct agactcccgc
     6421 cgatgagcgc cgctccgatc tccgacttcc gctccgacac cgtcacgaag ccgacccccg
     6481 cgatgcgccg cgcgatggcc gaggccgagg tcggggacga cgtctacggc gaggacccga
     6541 cgatcgcccg cctcgaggcg cggacggccg agctcctcgg cttcccggcc gccctcttcg
     6601 tccccaccgg ctcgatgggg aaccagatcg cgctccgcgt ccacgcccgg ccgggcacgg
     6661 aagtgatcgt cgaggcgcgg agccacgtgt tccactacga gatgggcgcg atggcggcgc
     6721 tgtcggggct cctcccgcgc ccggtgttcg ggccgggagg gcggatgccg gaggccgagg
     6781 cagaagcctg ggtgaggccc gagtccgtct actacctccc gcggacctcg ctcgtctgcc
     6841 tcgagaacac ccacaacttc gcagggggga cggtcctgcc tcgggccgcg gtggaggagg
     6901 tcctggcgct cgcccggagg aaggggctcg ccgttcacgt cgacggggcg cggctctgga
     6961 acgccgcggc cgcgctccgg gtctccgagg cgtcgctcgt ggcgggggtc gactcggtga
     7021 tggtctgctt ctcgaaaggg ctccgtgccc ccgtcgggag cgccgtggcc ggctcggagg
     7081 cgttcgtcgc cgaggcgcgc cgcgcgagga agctcttcgg cggagggatg cgccaggccg
     7141 gggtcctcgc cgcggcggcg ctcgtcgccc tcgaggaaga acgaggtcga ctcgccgagg
     7201 accacgcccg gctcgcccgg ctggcccgcg agctggcggg cgtccggggg gtctcggtcg
     7261 acccggagtc cttcccgacg aacatcctga tcgtcgacct cgacgcccgc gtctggggct
     7321 cggcgaacga cgccctcggg aagctgcgaa cgcgcggcat cctcgccggg gcggccggtc
     7381 cggcgtcggt ccgcctcgtg acgcacgccg acgtcggcga cgccgacgtg gaccgctgcg
     7441 tggcggcgtt ccgggagtcc gcgggcgtga gctgagagcg gggcggagcc cgccccctcc
     7501 tcaggcgccg gggagcttcg cgatcagctc gatctcgacg cggccattcc cggggagagc
     7561 gccgacctgg acggtcgagc gggccggccg gtggtcgccg aagcgggcgg cgtaggcgcc
     7621 gttcatctcg gcgaagctcc ccatgtcggt caggaagacg gtcgtcttga cgacgtcggc
     7681 gagcgtgcag ccggccccgg cgaggaccgc ctcgaggttg tcgaacacct ggttcgtctc
     7741 ctcggcgatc gtcccggaga cgagcttcat cgtggccggg tcgaggtgga cctgtccggc
     7801 gctgaaaagg aaccctccgg ccacgatccc ctgcgcgtag gggccgatgg ccttcggggc
     7861 gttctcgacg gcgatggtct tcagctggct catttgctcg ggtcccagat ggcgacgtag
     7921 ggaaggttac ggtacttctc gtcgaggtcg aggccgtagc cgacgacgaa ctcggggccg
     7981 atcgtgaagc cgaggaagtc gaccgggagg tcggcgttct cggggaggtc cttcttcagg
     8041 agcgcggcga tcctcacgga gcgcgggccc cgcgtcccga ggaggccgag gagcttgcgg
     8101 agcgtcaggc ccgtgtcgac gacgtcctcg aagatcagga cgtcgcgtcc cgcgatgtcc
     8161 gaggagaggt cgcggacgag ctcgaagttc ccggtggagg cggtcccgga gtacgacctc
     8221 acctggagga agtcgacggc caccggggtc gaaaggcgct tcaggaggtc ggtgaggaag
     8281 aagacggagc ccttcaggac gccgacggcg aggatctccc ggcccgcgta ggccgagtcg
     8341 atctcccggg ccagctcgtc caggcgggcc gagatctgct gctcggaaat gaggatgcgg
     8401 tcgatcacgg gacgaaacta taatgcaggc gcaactccga gaggttcacg tgagcacgtc
     8461 gatcgtcgat ctgaagacca ttcacgacgc ctacacgcgc ctgaccgaga agttcaagac
     8521 cttctggacg ttccatcagt tcctccaggg ggtgcacaag accttcttcg gcgacgcgcc
     8581 gggctacaac gtggatttcc aggggctcta cgacgagatc cgggccgtga cgacggtcat
     8641 gaacgtcgag ccgccgccgg tcgtcatcga ccggatccac cagctcgacg gccggctcga
     8701 cgtcgtctac cgggccctct cggcggacga cgagaagatc gcggcgagct gggtccgccg
     8761 cttcttcgag aaggtccgga cggaggacga gaagctcctc ctcgcgatcc tccgcttcta
     8821 cttccacgcg cgccaggtgt cgccggaagc gctcgacaag atggacttcc tgctgacgct
     8881 ggtcggggcg cgccggtcgc tcgacgacgg gagatacctg ccccggttcc cgcaggagct
     8941 cacgaagctc ttcgcctcgc tcctcgggct cgtccgccgc aatcccgcgc ccgcagccga
     9001 gctgacggcg tcggtcaagg ccctgggcct tctccggaag gacatcgact cgtgcgaacg
     9061 cttcgaggac ctcaccgaga agaagatcct cgacagcctc cg
//
LOCUS       DAOTEZ010000154         9027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5471_length_9027_cov_4.444383, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000154 DAOTEZ010000000
VERSION     DAOTEZ010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9027
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5471_length_9027_cov_4.444383"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="P5164_09555"
     CDS             <1..893
                     /locus_tag="P5164_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011556561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRY44178.1"
                     /translation="ALCGAEPVSPDALDHFARRFEPFGFDRRSLRPVYGLAEAALAVT
                     FTSGGREVRSLHVDPVRLAAAGEAVDGTRAIASVGTPVPGTDIEVRDPDGRVLEERRV
                     GRIFVRGPSMMAGYFEQAAATASVLAGGWLDTGDLGFFADGELYVCGREKNVVVIRGA
                     NHLPQEFEEALTGLEPVRTGCAVALGFVPDGEDGEQLLVLAERVRGSGPAADDSLVVD
                     AICRAILARTGIRPHTVRLLAPGTIPRTSSGKLRRGEALRRFLAGELDPPAKVSRLRI
                     LLEVLRSAVAFGRVRGNPGS"
     gene            1490..2080
                     /locus_tag="P5164_09560"
     CDS             1490..2080
                     /locus_tag="P5164_09560"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRY44179.1"
                     /translation="MSPNEKSQCQHRVYVDIPNLVLVGGHVAAVAKGLAPSVEVANTY
                     NVFDHLWRLDLRALVSRIAWSRERSATRLVAFGSSAGCDFGFWRAVQQAGMEAVILPR
                     VPGRREKGVDISLAMEALHDVENRLKPGDEITLVAGDGDYVPLVQRVLERGISVRVVF
                     WSSAAKALKEAASESVCLDPYLHRLTLAPYARRRAA"
     gene            2095..4089
                     /locus_tag="P5164_09565"
     CDS             2095..4089
                     /locus_tag="P5164_09565"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY44180.1"
                     /translation="MSSMNSEVRTTIEPYADGASCLEAWFRVLGAMDGFPVTDHPSGI
                     LPLEDRAEAFDLVQERTRATLEAGIPVPVLDGMETLGLDVLDQLVVLALLRNSLDARQ
                     SEGLRLRQICEASGAADWKRQETLRSRLERAGALRHLGLVQSDGDTVAAQRLYRLTPA
                     WKAPLLEGQAEPFELPVSVPETVTGRLQEALFRSVELLEWVAPTLWTRSSGWADAFGD
                     RPGWDYVGPRRRELVRVAASYLGPDGPAQGDPLGRLLESAGVATPAEVALLLLLLSRG
                     PKDGPLSWAFLEAALKGMPGAEAGFHGLLGRDSALVRGGLVALRSEESEGALAAFAAT
                     PRARGLAVPMGVAAFEPAKDDAAKENEGPSGFEATSPRIGLKEIVLTREARERIEEAL
                     ALPRALASGAADWGLDRSLLGQPGVVLLFHGEPGTGKTLCAEAIAGELGKALWRLRVD
                     ELLSRWVGETEQKVAAVFRRARAAGDVVLLDEADSVLSARNGQSARWETSMTNLLLQE
                     IERFPGVLVMTTNRPDSLDPALERRVLAKVEFEMPGEEERLELWKRHIPARIPFAPGV
                     DLAEVARKYALSGSGIRTAALFAVGRALGRPEGERLLTNRDLETAAAAQLARRPKAAP
                     VVGFTPMHEKRGGYAVAAGPRLEAPAVRPDDTIPRRERGR"
     gene            4173..4685
                     /locus_tag="P5164_09570"
     CDS             4173..4685
                     /locus_tag="P5164_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44181.1"
                     /translation="MKTKTTKPAVTTYDVAWYVHERDLAPGGSPVERPDVAILLDQSD
                     RPWFEIRGRHGRPRPIGRIRSVVPASLDFDGALLDVLLALWPERFETCPSLRAVEAKL
                     ARERMLDFHLGADRIPAEWAALREEARPHYERLTIVRVDAPIVGPESLLARSALAPVH
                     SLRPTHSDAI"
     gene            4742..5518
                     /locus_tag="P5164_09575"
     CDS             4742..5518
                     /locus_tag="P5164_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44182.1"
                     /translation="MASEAFPEALGFKNGDTSVHTARTMMLAELGDIFSAVPAEGSRE
                     DYWRAAIEDNALGKPSMVTRRSTIAHMTELYGLTPGIPLFRSLRRLWPQAGLGRPMLA
                     LLCACARDPLLRLTAQNALKAKEGATIPRAQVEEVIATGAPGRFSPKMIRSAAQNTSS
                     SWTQSGHFTGKAKKVRSRPRVTREAVAYALFLGHLCGLRGPALFTSFWASLLDRRPEE
                     ILTLAEEASAAGLLTLKQAGPVVEVGFPGWLTPKEEALLR"
     gene            5515..6072
                     /locus_tag="P5164_09580"
     CDS             5515..6072
                     /locus_tag="P5164_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HRY44183.1"
                     /translation="MNRVEALVKAWASHVAVPWTRGLAGGEKVWFCVYDKADERRLRL
                     HLPEFEGAAKKAGHGWAAVDLTDTFAAWMAAEENRDAYFEDPESLGSDKLVDFHEYLV
                     DLITPTLAEAGENDVVALTGVAALFGFLRVSELVHALEPHIRGRLVVFFPGEYENGNY
                     RFLDARDGWSYRAVPILPAEGAFAS"
     gene            6075..>9027
                     /gene="brxC"
                     /locus_tag="P5164_09585"
     CDS             6075..>9027
                     /gene="brxC"
                     /locus_tag="P5164_09585"
                     /inference="COORDINATES: protein motif:HMM:NF033441.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HRY44184.1"
                     /translation="MKNREVFQKDPLELRLVNNGVAEVVDASTDEQVRALRYELETFV
                     CEKQYSRGLHQILDSYLTCLGTEEQKPVWVSGFFGSGKSHLVKMLRALWVDVELPDGA
                     RARGIAHLSSEVKTSLKELSTQGKRLGGLHAASGSLLTEQGNSIRLALLGFVFRSAGL
                     PKSYPAARFVLFLRKNGWYEKVKREVEKAGKSFEKELRDLYVSPVLAKALLKADPGFA
                     ESEAKAKTLLKEQYPSRTDVTDDDMVHAIQDALEVDGRLPLTLVVLDEVQQWIGEDVE
                     RGAQVNSLAECLAKRFGARLLLVCTGQTALTGTPVLQRIQHRFHVKVELSDADVETVI
                     RKIVLAKRADRVAPLEKLLENCSGEVSRHLTETRIGPRPEDDSVLLPDYPLLPVRRRF
                     WEKVLRAVDRAGTAGQLRTQLRVVHEAVQRTAEAPIGTVVPADFVYYDRATELVQTGV
                     LLREIHEKVARLDDGTVDGSLKSRLAALAFLIGRLPREKGLDDGIRAPPETLANLLVE
                     DLKGGGDDLRGRVPKLLDAMAAAGHLMKVDDEYRLQTKEGSAWDAAWREAVARIQGDE
                     PKMASLRRDVLKDALEASLGGIAVHQGKSKVKRAAELSYGAERPSPAKGGIPVWIRDG
                     WNEDEKAVVAEVHSLGTEAPLVVVFVPKRRGEELKKALAAWKGADETLSARGIPTSEE
                     GKEARHGMEGTRESRRQEIEGLVAEIVGGARVFLAGAPKPLDGDDLEETVKDAVSAAL
                     ARLYPRFGDGDHLAWEQVFTKAKGGSPDALTAVGHKGDVAAHPVTSAVRAEIGSGRKG
                     SELRKKFAEPPFGWPQDAVDGALFALLAAGRIRATLQGQPAGVNLDRTKIGIAEFRLE
                     EDLVTTPQKIALRNLFQQAGLSGKPNEEVATASLFLDELRRRAEAAGGDKPLPERPSL
                     TRVNELAALTGNKQLLALHDGREELGKLAKEWKERGEAIGRRLPAWQTLERLRSHADG
                     LAVATEVAPQAE"
BASE COUNT         1348 a         3273 c         3108 g         1298 t
ORIGIN      
        1 acgccctctg cggcgccgag ccggtatcgc ccgacgccct cgatcacttc gccaggcgct
       61 tcgagccgtt cggcttcgac cggaggagcc tgcgtcccgt ctacggcctg gccgaggccg
      121 cgctcgccgt cacgttcacg tccggcggcc gcgaggtgag gtctctccac gtcgacccgg
      181 tgcggctcgc ggctgccggc gaggccgtgg acggaacgcg ggcgatcgcc tcggtcggga
      241 ccccggttcc gggcacggac atcgaggtgc gcgacccgga cggccgcgtt ctcgaggagc
      301 gccgggtggg tcggatcttc gtccgcggac cgtcgatgat ggcggggtac ttcgagcagg
      361 ccgccgcgac ggcgagcgtc ctggccggcg gctggctgga caccggggac ctcggatttt
      421 tcgccgacgg cgagctctac gtctgcggcc gcgagaagaa cgtcgtcgtc atccgcgggg
      481 cgaatcacct gccgcaggag ttcgaagagg cgctcacggg cctggagccg gtgcgaaccg
      541 ggtgcgcggt ggcgctcggg ttcgtcccgg acggagagga cggcgagcag ctcctcgtcc
      601 tggccgaacg cgtccgcgga agcggtccgg ccgccgacga ctccctcgtc gtcgacgcga
      661 tctgccgcgc gatcctcgcc cgcaccggca tccggcccca cacggtccgt ctcctcgccc
      721 cggggacgat cccgcgcacc tcgagcggca agctccgccg cggcgaggcg ctgcggcggt
      781 tcctggcggg ggagctcgac ccgccggcga aagtgtcccg cctccgcatc ctcctcgagg
      841 tcctccggtc tgcggtcgcg ttcgggaggg tgcgggggaa tcccggctcg tgaggcagag
      901 ggcctgaggc gggcctggag agccccaggc ggccgtaccc atcgagcgcc gggggggctg
      961 ctcgcagtac ccgcgtacgg cgaggggcct gtcgaggatc gaacgatcac tcttcagatc
     1021 gggggtgccc cggggaggag ccgctggccc gtcgcgtgtc cgacgccacc tccaggcccc
     1081 tacgtcgtgt cacccgcggt gttgaacctc tggctcggat ttggtagaat ccgcggcatg
     1141 ctggagcgac gtgccaacgg cggagggaag cccattttca gtgggctcag ccgacgtccg
     1201 gtcttcagtg tcgctggtgt tcccgggaaa gtcagttagc caattatcta aagcagcatc
     1261 aatttccgta gacacttttc ggttccgaat acgaaagatg aggaggccgt tcgtgagtcg
     1321 tcttcgccgc atgtccggtc ccgccgaaac gaggtctccc accctgatag cgaagtgcga
     1381 gtagtaggac gagagttctc gttgtcttcc attccgtaaa caggaattgt ctttcccgag
     1441 aacacgcatt tccccgttga acagcgggaa atcaagtaag gaaaagagga tgagcccgaa
     1501 cgagaagagc cagtgccaac accgcgtcta cgtcgacatc ccgaacttgg tcctcgtggg
     1561 cggccacgtg gctgccgtcg cgaagggcct cgcgccgtcg gtcgaggtcg cgaacaccta
     1621 caacgtgttc gaccatctct ggaggctcga tctcagggcc ctcgtgtcgc gcattgcgtg
     1681 gtccagggag cggagcgcca cgcggctggt ggccttcggc tcttcggccg gctgcgactt
     1741 cggcttctgg cgcgccgtcc agcaggccgg gatggaggcc gtgatcctgc cgcgagtgcc
     1801 tggccgccgc gaaaaggggg tcgacatctc gctcgcgatg gaggccctcc acgacgtgga
     1861 gaaccgcctg aagcccgggg acgagatcac gctcgtcgct ggagacggcg actacgtccc
     1921 gctcgttcag cgcgtccttg agcgcgggat ctccgtccgc gtcgtgttct ggtcctcggc
     1981 cgcgaaggcg ctgaaagagg ccgcctccga gtctgtctgc ctggacccct accttcaccg
     2041 cctgacgctc gctccgtacg cccgccggcg ggccgcctga gaggaggaga cgagatgagc
     2101 tccatgaact ccgaagtccg aaccacgatt gagccctatg cggacggcgc atcctgtctc
     2161 gaggcctggt tccgagtcct cggagccatg gacggctttc ccgttaccga tcacccctct
     2221 gggatcctcc cgctcgagga tcgcgcggag gcgttcgatc tcgtccagga gcggacgcgt
     2281 gccacgctgg aggccggaat cccggtcccg gtcctcgatg ggatggagac gctcgggctc
     2341 gacgtcctcg accagctcgt cgtcctcgcc ctgctgcgga actcgctcga cgcccgccag
     2401 agcgagggcc tgcggcttcg ccagatctgc gaggcctcgg gcgccgccga ctggaagcgg
     2461 caggagaccc tccgctcacg gctggagcgg gccggcgcgc tccggcacct cgggctcgtt
     2521 cagtccgacg gcgacacggt cgcggcgcag aggctctacc ggctgacgcc cgcgtggaag
     2581 gcgccgctcc tcgaggggca agccgagccc ttcgagttgc cggtgtccgt gcccgagacc
     2641 gtcactgggc gcctgcagga agcgctcttc cgttccgtcg agctccttga gtgggtggcg
     2701 ccgacgctct ggacgcggtc gtccggctgg gccgacgcct tcggcgatcg cccggggtgg
     2761 gactacgtcg gcccgcggcg gcgcgagctc gttcgcgtcg ccgcctccta cctcgggccc
     2821 gacggtccgg cgcagggcga cccgctcggt cgtcttctcg agtccgcggg ggtcgccacg
     2881 ccggccgaag tcgcgctgct tctgctcctc ctttcgcgcg gcccgaagga cgggccgctc
     2941 tcgtgggcct tcctcgaggc ggcgctgaag ggaatgccgg gcgccgaggc cggcttccac
     3001 ggtctcctcg gccgcgactc cgcgctcgtt cggggcggcc tcgtcgcgct ccgctccgag
     3061 gagtcggagg gcgctctcgc cgccttcgca gcgacgcctc gggcgagggg gctcgcggtc
     3121 ccgatgggcg tcgccgcctt cgagcccgcg aaggacgacg ccgcaaagga gaacgagggc
     3181 ccgtccggct tcgaagccac ctctccgcgc atcggcctga aggagatcgt cctcacccgg
     3241 gaggcgcggg agcggatcga ggaggcgctc gcccttccgc gcgcgctcgc gtccggagcg
     3301 gccgactggg gcctcgaccg gagcctgctc ggccagccgg gcgtggtgct cctcttccac
     3361 ggcgagcccg gcacggggaa gacgctctgc gccgaagcga tcgcgggaga gctcggcaag
     3421 gccctctggc ggctgcgcgt cgacgagctc ctctcgcgct gggtggggga gaccgagcag
     3481 aaggtggccg ccgtctttcg ccgggcccgc gcggccggcg acgtcgtcct cctcgacgag
     3541 gcggactcgg tcctctcggc gaggaacggc cagagcgcgc gctgggagac ctcgatgacg
     3601 aacctgctcc tgcaggagat cgagcggttc cccggcgtgc tcgtcatgac gacgaatcgt
     3661 cctgactccc tcgatccggc gctcgagcgc cgcgtcctcg cgaaggtcga gttcgagatg
     3721 cccggcgagg aggagcggct cgagctctgg aagaggcaca tccccgcccg aatcccgttc
     3781 gccccgggag tcgaccttgc cgaggtcgca aggaagtacg ccctctcggg ctccggcatc
     3841 cgaacggccg ccctcttcgc ggtcgggcgg gcgctgggcc ggcccgaggg cgagcgcctc
     3901 ctcacgaacc gcgatctcga gaccgccgcg gcggcgcagc tggcacggag gccgaaggcg
     3961 gctcccgtcg tcgggttcac cccgatgcac gagaagcggg gcggctacgc ggtcgcagcc
     4021 gggccccggc tggaagcgcc ggccgtgagg cccgacgaca ccatcccccg tcgggagcga
     4081 ggccgatgag gctctctcgc ccggcggggc ataccgaatc accactcacg aatcgggatc
     4141 tggcccgtac gccagaagaa ggagagacca ccatgaagac caagaccacc aagcccgctg
     4201 tgaccaccta cgacgtcgcc tggtacgtcc acgagcgcga cctcgccccc ggaggctcgc
     4261 ccgtcgagcg gccggacgtc gcgatccttc tcgaccagag cgaccggccc tggttcgaga
     4321 tccgcggccg gcacgggcgt ccccggccca tcggccggat ccgctccgtc gtccccgcca
     4381 gcctcgactt cgacggcgcg ctcctcgacg tgctcctcgc gctgtggccg gagcgcttcg
     4441 agacatgccc ctcgctccgg gccgtcgagg cgaagctcgc gcgagagagg atgctcgact
     4501 tccacctcgg cgcggaccgg atcccggccg agtgggcggc cctccgcgag gaggcacggc
     4561 cccattacga gcggctcacc atcgtccgcg tcgacgcccc gatcgtcggg cccgagtcgc
     4621 tcctggcccg ctccgccctc gctcccgtac actcccttcg cccgacccac tcggacgcca
     4681 tttgaccggg tcgcggcgct atctcgcggg gcggaggtgc gatccgtgcg gagggtgctc
     4741 gttggcctct gaagcgttcc ccgaggccct cggtttcaag aacggcgaca ccagcgtcca
     4801 cacggcgcgg acgatgatgc tcgccgagct gggcgacatc ttctcggccg taccggcgga
     4861 agggagccgc gaggattact ggcgggccgc gatcgaagac aacgccctgg gcaagccgag
     4921 catggtgacg cggcgctcga cgatcgccca catgacggag ctgtacggcc tgacgccggg
     4981 cataccgctc ttccgcagcc tccgccgtct ctggccgcag gccggcctcg gccgcccgat
     5041 gctcgcgctt ctctgcgcct gcgctcgcga cccgctcctc cgcctgacgg cgcagaacgc
     5101 cctgaaagcc aaggaggggg cgactattcc ccgcgctcag gtggaggagg tcatcgccac
     5161 aggtgcgccg ggccggttca gtccgaagat gatccgctcg gctgcccaga atacgagctc
     5221 gtcctggacg cagtcggggc acttcacagg aaaggcgaaa aaggtccgtt cccgcccgcg
     5281 cgtcacccgc gaggccgtgg cctacgccct cttcctcggg cacctctgcg ggctgcgcgg
     5341 cccggcgctc ttcacgagct tctgggcctc gctcctcgac cgccgccccg aggagatcct
     5401 gactctcgcc gaggaggcgt cggccgcggg cctcctgacc ctcaagcagg ccggccccgt
     5461 cgtcgaagtc gggttccccg gctggctcac ccccaaagag gaggcgctcc tgcgatgaac
     5521 cgcgtcgaag ccctcgtgaa ggcctgggcc tcccacgtcg ccgtcccctg gacccggggc
     5581 ctcgccggcg gcgagaaggt ctggttctgc gtctacgaca aggccgacga gcgccgcctg
     5641 cgccttcacc tccccgagtt cgagggagcg gcgaagaagg ccggccacgg ctgggcggcc
     5701 gtcgacctga ccgacacctt cgccgcctgg atggccgccg aggagaaccg ggacgcctac
     5761 ttcgaagatc cggagtcgct cggaagcgac aagctcgtcg actttcacga gtacctcgtc
     5821 gacctcatca cgccgaccct cgccgaggcg ggcgagaacg acgtcgtcgc cctcaccggc
     5881 gtcgccgccc tgttcggctt cctccgcgtc tccgagctcg tccacgccct cgagccccac
     5941 atccggggcc gtctcgtcgt cttcttcccc ggcgagtacg agaacggcaa ctaccgcttc
     6001 ctcgacgccc gcgacggctg gagctaccgg gccgtcccga tcctgcccgc tgaaggagcg
     6061 ttcgcgtcat gacgatgaag aaccgcgaag tcttccagaa ggaccccctc gagctccggc
     6121 tcgtcaacaa cggcgtcgcc gaggtcgtcg acgcctccac cgacgagcag gtccgggcgc
     6181 tccgctacga gctcgagacc ttcgtctgcg agaagcagta ttcccggggc cttcaccaga
     6241 tcctcgacag ctacctcacc tgcctcggga cggaagagca gaagccggtc tgggtgagcg
     6301 gcttcttcgg gagcggcaag tcgcacctcg tcaagatgct ccgcgccctc tgggtcgacg
     6361 tcgagctgcc cgacggcgcc cgcgcccgcg gcatcgccca cctttcctcg gaggtgaaga
     6421 cgtccctcaa ggagctttcc acgcaaggga agcgcctcgg cgggctgcat gcggcgagcg
     6481 gcagcctcct caccgagcag gggaacagca tccgcctcgc cctcctcggg ttcgtcttcc
     6541 gctccgccgg cctcccgaag agctacccgg ccgcgcgctt cgtcctcttc ctgaggaaga
     6601 acggctggta cgagaaggtg aagcgggagg tcgagaaggc aggcaagagc ttcgagaagg
     6661 agctccgcga cctctacgtc tcgcccgtcc tcgccaaggc cctcctgaag gccgacccgg
     6721 gattcgccga gtcggaggcg aaggcgaaga cgctgctgaa ggagcagtac ccgagccgga
     6781 ccgacgtcac cgacgacgac atggtccacg ccatccagga cgccctcgag gtcgatgggc
     6841 ggctgccgct cacgctcgtc gtcctcgacg aggttcagca gtggatcggg gaggacgtgg
     6901 agcgcggcgc ccaggtcaac agcctcgccg agtgcctcgc caagcggttc ggggcgcggc
     6961 tcctcctcgt ctgcaccggc cagacggccc tcacggggac gcccgtcctc cagcgaatcc
     7021 agcaccgctt ccacgtcaag gtcgagctct ccgacgccga cgtcgagacc gtcatccgga
     7081 agatcgtcct cgccaagcgc gcggaccgcg tcgccccgct cgagaaactc ctcgagaact
     7141 gcagcggcga ggtctcgcgg cacctcaccg agacgcggat cgggccccgc cccgaggacg
     7201 attccgtcct cctccccgat tacccgctcc tccccgtgcg gcgccgcttc tgggagaagg
     7261 tccttcgagc cgtcgaccgg gccggcacgg cgggccagct ccggacgcag ctccgcgtcg
     7321 tccacgaggc cgtccagagg acggccgagg ctccgatcgg caccgtcgtc ccggccgact
     7381 tcgtctacta cgaccgcgcg accgagctcg tccagacggg cgtccttctc cgcgagatcc
     7441 acgagaaggt cgcccgcctc gacgacggca cggtggacgg ctcgctgaag tcgcgcctcg
     7501 cggccctcgc cttcctcatc gggcgcctcc cgcgcgagaa gggcctcgac gacggcatcc
     7561 gggccccgcc cgagacgctc gcgaacctcc tcgtcgaaga cctgaagggg ggcggggacg
     7621 acctccgcgg ccgcgtcccg aagctcctgg acgcgatggc ggcggccggc cacctgatga
     7681 aggtggacga cgagtaccgc ctgcagacaa aggaagggag cgcctgggac gctgcctggc
     7741 gcgaggccgt cgcccggatc cagggcgacg agccgaagat ggcgagcctt cggcgcgacg
     7801 tcctgaagga cgcgctcgag gcgagcctcg gcggcatcgc ggtccaccag gggaagtcga
     7861 aggtcaagcg cgcggccgag ctctcgtatg gcgccgagcg cccctcgccg gcgaaggggg
     7921 gcatccccgt ctggattcgc gacggctgga acgaggacga gaaggccgtc gtcgccgagg
     7981 tccacagcct cggcaccgag gccccgctcg tcgtcgtctt cgtcccgaag cgccggggcg
     8041 aggagctgaa gaaagcgctt gccgcctgga agggggccga cgagacgctg agcgcaagag
     8101 gcatcccgac ctccgaagag gggaaggaag cccgccacgg catggagggg acccgcgaga
     8161 gccgccgcca ggagatcgag ggccttgtcg cggagatcgt cggcggcgcc cgggtcttcc
     8221 tcgccggagc gccgaagccc ctcgacgggg acgacctcga ggaaacggtg aaggacgccg
     8281 tgtcggcggc cctcgcccgc ctctatcccc ggttcggcga cggcgaccac ctcgcctggg
     8341 agcaggtctt cacgaaggcg aagggcggaa gccccgacgc cctcaccgcc gtcggccaca
     8401 agggcgacgt ggccgcccat cccgtcacct cggccgtccg cgccgagatc gggagcgggc
     8461 ggaaggggag cgagctgcgg aagaagttcg cggagccgcc gttcggctgg ccgcaggacg
     8521 ccgtcgacgg cgccctcttc gccctcctcg ccgcgggccg catccgcgcg acgctccagg
     8581 ggcagccggc gggcgtgaac ctcgaccgga cgaagatcgg catcgccgag ttccgcctcg
     8641 aagaggatct cgtcacgacg ccgcagaaga tcgccctgag gaacctcttc cagcaggcgg
     8701 gcctctccgg aaagccgaac gaggaggtcg ccaccgcgtc gctcttcctc gacgagctcc
     8761 ggaggcgagc cgaggcggca ggcggcgaca agcccctccc cgagcgcccg tcgctcacgc
     8821 gcgtcaatga gctcgccgcc ctcacgggca acaagcagct cctggccctc cacgacggtc
     8881 gcgaggagct cggaaagctc gcgaaggagt ggaaggagcg cggcgaggcg atcggccggc
     8941 gtctcccggc gtggcagacg ctcgaacggc ttcggagtca tgccgacggc ctcgccgtcg
     9001 ccaccgaggt ggctccgcag gccgagg
//
LOCUS       DAOTEZ010000155         9023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5475_length_9023_cov_6.674844, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000155 DAOTEZ010000000
VERSION     DAOTEZ010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9023
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5475_length_9023_cov_6.674844"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="P5164_09590"
     CDS             <1..953
                     /locus_tag="P5164_09590"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRY44185.1"
                     /translation="ALARTAGEPADARQVEMERHLEGCASCRAAAAALDETWERIALP
                     EPDVSVAFRERTIGRMQEALARRSVVPFRPRTWWRPVLQAAALLVTGVGGFLLARASA
                     PPAGFPVAAAERAGTSSVAVVSEKQLDASRVALDLSGNPRLLNVAFRPSEGDGRLSIS
                     FDVTTRYHVSGRAEDRAVAALLAYVVNAGAVTEGARGRAIDLVSRELGERAAASPEIV
                     EVLVRTLAADGNPGVRKKAAEALVQLPPTPAIRDALALALRKDTNPAVRMIAVDGLAK
                     AATTLRDPLSIETLREKAGDETEPGFVRVKAASALREIPL"
     gene            1096..3063
                     /locus_tag="P5164_09595"
     CDS             1096..3063
                     /locus_tag="P5164_09595"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HRY44186.1"
                     /translation="MTFGDLERLTGMNTETTMSTLNTRAGSRTWTVLWILLIGAFLVV
                     SLAWPARGDRLGATASRTETFGAAAGVRSLTIDGVSGDVEVTAGASFAATVEVTVKAD
                     GEVRAREILGKTKIAFENVGGELSLATVEPGAEYSREGRRTRLRVSRDGDRRWRVEAR
                     YRVTLPAGASATVSLVNGGIVTRGLDGVQELTTVNGRVAASGARRSATLKSVNGGVEG
                     AFTALPRDARVVAETVNGGVKLMLPPDAAFDLKASAINGGIRSTFLLPVRGGADDDVA
                     VREIEAAAHAEAERARAAADRARERARKARAAGSSADETEDSWDREWSEFGREMAEFG
                     REMARLSREISRSVSGSLNRSYEGTHRGGGAAVKCSTINGGVLVLAAGSAESEARSLV
                     PKRGEGWAGERVAPVPPVPPVPPVPPVYAGHGRGREDGSVVKGDVTGDLQLSLPTGDV
                     RAGRVSGRATVRTSSGDIRIGAVGKGADLSSSGGDVRVERVDGGLTASTLGGDIVVGD
                     AAGEIRLRTMGGDVRLRSASGPVTAKTAGGDVRILRALAAVRAETAGGEVFCELASRD
                     AGPVDLSTGGGDVTVVLPANFRGDVDVVVRSVDADGDYVVSDFPEIGVVRHRGAGRGV
                     VRAEGKLNGGGAKVTVSASSGTVHIRKGPPA"
     gene            3200..3724
                     /locus_tag="P5164_09600"
     CDS             3200..3724
                     /locus_tag="P5164_09600"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRY44187.1"
                     /translation="MKRIVSAALVVVAALVASPASFAAVPEKGSIAIFAGIGPAIPFE
                     GDYEVGFHLEAGGEYYLSNVLALRGTLGLTRSNADGGSGVTLGGLEASAAYYARRGKW
                     SPYVLGGVGIHTVDPPGRGASQRLSAHVGGGTEYYLDRRTALTGEVIGRFVGSAGGRT
                     SSFASLAVGIKYHF"
     gene            3858..4235
                     /locus_tag="P5164_09605"
     CDS             3858..4235
                     /locus_tag="P5164_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44188.1"
                     /translation="MTRPRRRTATRAVALLVAVLTVLGVQLFLPAAAVACGPVMPDCC
                     CAGEASVDPAPAEGCDCSISQPAPVPAAEIAPSVVPVPPLIPTETADDLLAAKPAIAW
                     RPAVPPSRTRAAPTLALLETFRN"
     gene            4307..5689
                     /locus_tag="P5164_09610"
     CDS             4307..5689
                     /locus_tag="P5164_09610"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRY44189.1"
                     /translation="MILVPLSRRRAASKASAVAVLTLSLATSAVALVDLPPAASTPDR
                     EAAPTVETLVAEALERSPALAAARAAVAAARERISPAGTLPDPMLRLSYENDGTSLSL
                     GEEPMTRLSFMAEQAIPWPGKLGLAARVAAADADVAEAAIGRLRLGVEAEVRRAATRL
                     LEARAAGELTREQTENWERIESVARHRYAAGLGAQQDVFRAQAERTRLLQQQRRDEGA
                     ARAAEAELRRLLLREPGTPVEVGERIGEMRPEAPDANFERSRSEAESPELDAARRAAD
                     REALAIELARRDFKPDLLASAGYMNRGSLPMMWSVGLGVTVPLFSGSRQEPRVREARA
                     RLAAVKAAEADVRLRLRARTETQLALLAQLAEEARLDREALLVQDRMAVEAALASYAS
                     GSVPFVTVLEAISTEFADRRAALARLAAFRIAEADLRERSLDGAPMPAASSPLSAPAS
                     PAAMSAAGGM"
     gene            5701..>6635
                     /locus_tag="P5164_09615"
     CDS             5701..>6635
                     /locus_tag="P5164_09615"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY44190.1"
                     /translation="MKRTRISLFALVAALAVPALYLAAGCGGAKTQVADTKTYICPMH
                     PEVVKDGKGSCPICGMDLVPKEEAPVAPAAAHETWICPMHPEIVKDRKGSCPICGMDL
                     VRKEEGDSAGGAGVAGVPGFATVTIDARKRQLLSLKTAKVERGTFGTGIRTVGRVAFD
                     ERRVHHVHTRYEAYVEHVAADFTGKHVRRGEVLAHVYSPELFATQQEVLLALRAVRAL
                     GPQADPSAREGAERLLEAARQRLRLWEVTEKDIAALEARGEPLRSVPIYAPSSGYVTA
                     RTAFHGMKVMPADTLFDIVDLSSVWVLADVYESELP"
     assembly_gap    6636..6735
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6736..7032
                     /locus_tag="P5164_09620"
     CDS             <6736..7032
                     /locus_tag="P5164_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY44191.1"
                     /translation="AVLDSGTRKVVFVAKGEGVLEPREVKVGEQGGGAWEILSGLAEG
                     EEIALGASFLVDSESRLAAALAGMRDGATPATGPEGHVAAPAPPPSAPHDGHEH"
     gene            7043..>9023
                     /locus_tag="P5164_09625"
     CDS             7043..>9023
                     /locus_tag="P5164_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002734646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRY44192.1"
                     /translation="MIRKIIRFSAENKYLVLALWAVAVLLAFRALRTVPLDAIPDLSD
                     TQVIVLSRWDRSPDILEDQVTYPIVTALLGAPKVKTIRGFSDFGYSFVYVIFEDGTDL
                     YWARSRVLEYLSKIQGSLPSGVKTEIGPDATGVGWVYQYALVDETGKTSLDELRSYQD
                     WFLRYQLQSVPGVAEVATVGGMARQYQVTVDPNRLAAYAISLEAVSDALQRSNDEVGG
                     RLIEISGREYMVRGRGYLKGAADIEKVVVKTDERGTPVTIKDVGSVAVGPDLRRGLAE
                     LDGKGETVGGIVVMRSGENALHVIERVKERIDELRPSLPPGVELVTTYDRSTLIESAI
                     DNLKGKLVEEIVIVSIVILIFLWHVPSALVPILTIPVSVLLAFIPLHLMGITVNIMSL
                     GGIAISIGVLVDGAIVEVENAYKKLQIWNDSGRKGDFHAVRLEALMEVGPSVFFSLLV
                     IAVSFLPIFTLVDQEGRLFTPLAWSKNLAMALAAVLAITLDPAMRMLFARMDPFRFGF
                     AHDGQGALARLVRLPARLVEKAVNAVAVGTYHAEERHPVSKVLFRLYDPVCRAVLRHP
                     KKVVAVSLLVLVSTVPVYLSLGSEFMPPLHEGTVLYMPTTLPGISVTEAAKLLQTQDR
                     VLATFPEVERVFGKAGRAETSTDPAPLSMMETTVIL"
BASE COUNT         1188 a         3188 c         3280 g         1267 t
ORIGIN      
        1 ccgccctcgc ccgcaccgcg ggcgagccgg cggacgcccg tcaggtcgag atggagcgcc
       61 acctcgaggg gtgcgcgagc tgccgggccg cggcggcggc cctcgacgag acgtgggagc
      121 ggatcgcgct tccggagccg gacgtctccg tcgcgttccg cgagcggacg atcggacgga
      181 tgcaggaggc gctcgcccgc cggagcgtcg tgccgttccg gccccggacg tggtggagac
      241 cggtgcttca ggccgcggcc ctcctggtga cgggagtcgg cggcttcctg ctggcgcggg
      301 cgagcgcccc gccggcaggc ttccccgtcg ccgcggccga gagggccggg acgtcctccg
      361 tcgccgtcgt ctccgagaag cagctcgacg cgtcgcgcgt cgcgctcgac ctgtccggga
      421 atccacggct tctgaacgtc gcgttccgcc cgtccgaagg ggacgggcgc ctctcgatct
      481 ccttcgacgt cacgacccgc taccacgtgt ccggccgggc cgaggaccgg gccgtcgccg
      541 cgctgctcgc ctacgtcgtg aacgccggtg cggtgaccga gggggcgcgc ggccgggcca
      601 tcgacctcgt ctcccgcgag ctcggcgagc gcgccgccgc ctcgcccgag atcgtcgagg
      661 tcctcgtccg gaccctcgcg gccgacggga atccgggcgt gaggaagaag gcggccgagg
      721 cgctggtcca gctgccgccg acgcccgcga tccgcgacgc cctggccctg gcgctccgga
      781 aggacacgaa ccccgccgtg cggatgatcg ccgtcgacgg actcgcgaaa gcggccacga
      841 ctttgcgcga cccgctcagc atcgagacgc tccgcgagaa ggcgggagac gagacggagc
      901 ccggcttcgt ccgcgtgaag gcggcctcgg cgctgcggga gatcccgctc tgacgtggat
      961 cggggggacc cgcaccgccg ggtccccccg cggcgccttc ggcgcctccc cctccgcgcg
     1021 gcgacggcga gggcgaggtg cgtggatcgt gggggcgtcg cactcaccgg gtctctcctg
     1081 ccgcaaggtc ggagcgtgac cttcggtgac ctcgaacgtc tcaccggcat gaacacggag
     1141 accacgatgt cgacgctgaa cacccgcgcc ggttcccgca cctggacggt actctggatc
     1201 ctcctgatcg gagccttcct cgtcgtttcg ctcgcctggc ccgctcgggg cgaccggctc
     1261 ggagcgaccg cgagccggac ggagacgttc ggcgccgccg cgggcgtccg gtcgctgacg
     1321 atcgacggcg tcagcggcga cgtcgaggtc acggcggggg cgtcgttcgc ggcgaccgtc
     1381 gaggtgacgg tcaaggccga cggcgaggtc cgcgcgcggg agatcctggg gaagacgaag
     1441 atcgccttcg agaacgtcgg cggcgagctg tctctcgcca cagtcgagcc gggagcggaa
     1501 tactcgcggg aggggcggag gacacgcctt cgggtctccc gcgacggcga ccggcggtgg
     1561 cgcgtggaag cccgttacag ggtgacgctc cccgcgggcg cgagcgcgac ggtctcgctt
     1621 gtgaacgggg ggatcgtcac gcggggcctc gacggcgtcc aggagctgac gacggtcaac
     1681 gggcgcgtcg cggcgtccgg cgcccgccgg agcgcgacgc tcaagagcgt gaacggcggc
     1741 gtggagggag cgttcacggc ccttccgcga gacgcgaggg tggtcgccga gacggtgaac
     1801 ggcggcgtga agctgatgct tccccccgac gcggcgttcg acctgaaggc ctcggcgatc
     1861 aacggcggca tccgttcgac gttcctgctg ccggtgcggg gcggggcgga cgacgacgtc
     1921 gcggtccggg agatcgaggc ggcagcgcat gccgaggcgg aacgggcccg ggccgcggcc
     1981 gaccgggcgc gggagagggc acggaaggcg cgggccgccg gctcctccgc cgacgagacc
     2041 gaggactcct gggatcggga gtggagcgag ttcggccgtg agatggcgga gttcggccgt
     2101 gagatggccc gcctgagccg cgagatctcc cgctcggttt ccggcagcct gaaccgttcc
     2161 tacgaaggga ctcaccgggg cggcggagcg gccgtgaagt gctcgacgat caacggcggc
     2221 gtcctcgtcc tcgccgccgg cagcgcggaa tcggaggcga ggtccctggt gccgaagcgc
     2281 ggcgaggggt gggcgggtga acgggtcgca ccggtgcctc ccgttccccc cgtgcctccc
     2341 gtgcctcccg tgtacgcggg ccacggtcgc gggcgcgagg acggctcggt cgtgaagggg
     2401 gacgtgaccg gcgacctcca gctctccctc ccgaccgggg acgttcgtgc cgggagggtc
     2461 tccggccgcg cgaccgtgcg gacgagctcg ggcgacatcc gtatcggggc cgtcgggaag
     2521 ggagccgacc tttcgagctc gggcggggac gtccgggtcg agcgggtcga cggcgggctc
     2581 acggcgtcga cgctcggcgg cgacatcgtc gtgggagacg ccgccgggga gatccgcctc
     2641 cggacgatgg gcggcgacgt gaggctccgt tccgcgagcg gtccggtcac cgcgaagacg
     2701 gcgggcggcg acgtccggat cctccgggcg ctcgccgcgg tacgggcaga aaccgccggc
     2761 ggcgaggtct tctgcgagct cgcgtcgagg gatgccggcc cggtggatct gtcgaccggg
     2821 ggcggcgacg tgaccgtggt ccttccggcg aacttccgcg gcgacgtcga cgtggtcgtg
     2881 cggagcgtcg acgcggacgg ggactacgtc gtgtccgact tccccgagat cggcgtcgtt
     2941 cggcatcgag gcgccggccg cggagtcgtg cgcgcggaag ggaagctgaa cgggggcggt
     3001 gcgaaggtca ccgtcagcgc ctcctccggg accgttcaca tccggaaggg accgcccgcc
     3061 tgaggacggg tcgcgtggcc cgagccggtc ggcgccgcgc ccccgcttcg gcgggggcgc
     3121 ggccgtttgc taccctccgg tccggcgccg gcgcctcgcg ccgggtccgg ccccgcgaat
     3181 ccagctcctg gaggaagcca tgaagagaat cgtctctgca gccctcgtcg tcgtcgcggc
     3241 gctcgtcgcc tctcccgcgt cgttcgccgc ggtcccggaa aagggctcca tcgcgatctt
     3301 cgccggcatc ggacccgcga tcccgttcga aggcgactac gaagtcggct tccacctcga
     3361 ggcgggaggc gagtactacc tctcgaacgt cctcgcgctc cgcggcaccc tcggcctgac
     3421 gcgttcgaac gccgacggag gctcgggcgt cacgctcggc ggcctcgagg cgtccgccgc
     3481 ctactacgcg cgcaggggga agtggagccc ctacgtcctc ggtggggtcg gtatccacac
     3541 ggtcgacccg ccggggcggg gcgcctctca gcggctcagc gcccacgtcg gcggcgggac
     3601 ggagtactac ctcgaccgac ggacggccct gacgggcgag gtcatcggtc gcttcgtcgg
     3661 cagcgccggc ggccggacgt cgagcttcgc ttcgctcgcg gtcgggatca agtaccactt
     3721 ctgagacaca agttgttgat tgggaacggg ttcgttcgga gatgccggcg cccgcagggg
     3781 tgcgtggtcc cgcgatgaga agggtctctt ggagaactgg acttgacagg tccgaattct
     3841 cgcctacctt gtctgcgatg acgcgccccc gtcgccggac ggcgacccga gccgtggctc
     3901 tcctggtggc cgtcctgacg gtcctcgggg tgcagctgtt cctgccggcg gctgccgtcg
     3961 cgtgcggccc cgtgatgccg gactgctgct gcgcgggaga ggcctcggtg gatccggcgc
     4021 ccgcggaggg ctgtgattgc tccatctcgc agccggcccc ggtgccggcc gcagagatcg
     4081 cgccgagcgt ggtgcccgtg ccgccgctga tcccgaccga gacggcggac gatctcttgg
     4141 cggcgaagcc ggcgattgcg tggcggccgg ccgttccgcc gtcccgcacc cgcgccgccc
     4201 cgaccctggc tctcctcgag acgttccgaa actgagactc ccggagatcg agctctcgcc
     4261 caccccgtcc ggggtggccc gatgaccgtt ttcccgaggt gtcgagatga tcctggttcc
     4321 gctctcccgg cggagggccg cgtcgaaggc ctctgccgtc gccgtcctca ccctttccct
     4381 ggccacgtcg gccgtcgcgc tcgtcgatct gccgcccgcc gcttccacgc cggatcggga
     4441 ggccgcaccg accgtcgaaa ccctcgtcgc cgaagcgctc gagcgaagcc cggccctcgc
     4501 cgcggcgagg gccgccgtcg cggccgcgcg cgagcggatc tccccggccg ggacgttgcc
     4561 cgacccgatg ctccggctct cctacgagaa cgacgggacg tccctctcgc tcggcgagga
     4621 gccgatgacc cggctctcgt tcatggccga gcaggcgatc ccgtggccgg gaaagctggg
     4681 cctcgcggcg agggtcgccg cggccgatgc ggacgtcgcg gaggcggcga tcgggcgact
     4741 ccggctgggc gtcgaggccg aggtccgacg ggccgcgacg cgcctcctcg aggcgagggc
     4801 ggcgggcgag ctgacccgcg agcagaccga gaactgggag cggatcgagt cggtcgcgcg
     4861 ccaccgctac gcggccggtc tcggggcgca gcaggacgtc ttccgcgcac aggccgagcg
     4921 gacgcgcctc ctccagcagc agcgccgcga cgaaggggcc gcgcgcgcgg ccgaagccga
     4981 gctgcggagg ctcctccttc gggagccggg gacgccggtc gaggtcgggg agcggatcgg
     5041 cgagatgcgg cccgaagcgc ccgacgcgaa cttcgagcgg tcgcggtccg aagccgagag
     5101 ccccgagctc gacgcggctc ggagagcggc cgaccgcgag gcgctcgcga tcgagctcgc
     5161 gcgccgcgac ttcaagcccg acctcctcgc ctcggccggc tacatgaacc gcggctcgct
     5221 tccgatgatg tggtccgtcg gcctcggggt gacggtgccg ctcttctccg ggagccggca
     5281 ggagccgcgg gtccgagagg cccgggcacg gctcgccgcc gtgaaggccg cggaggccga
     5341 cgtccgcctc cgcctccggg cgcggacgga aacgcagctc gccctcctcg cccagctcgc
     5401 cgaggaggcg cggctcgacc gggaggcgct cctcgtccag gaccggatgg cggtcgaggc
     5461 ggccctcgcg agctatgcct ccggctccgt ccccttcgtc accgtcctcg aggcgatctc
     5521 gaccgagttc gcggaccggc gcgcggccct cgcgcgcctc gccgccttcc gtatcgcgga
     5581 ggccgacctg cgcgagcgat cgctcgacgg cgcgccgatg ccggcggcct cgtcccccct
     5641 ttccgcccct gcatcgccgg ccgcgatgag cgccgccggc gggatgtgag gagacctccg
     5701 atgaaacgca cccgaatctc tctcttcgcg ctcgtggccg cgcttgccgt tccggcgctc
     5761 tatctcgccg cgggctgcgg aggagcgaag acgcaggtcg ccgacacgaa gacctacatc
     5821 tgcccgatgc acccggaggt cgtgaaggac gggaagggga gctgcccgat ctgcggcatg
     5881 gacctcgtcc cgaaggagga ggccccggtc gcgccggccg ccgcgcacga gacctggatc
     5941 tgcccgatgc acccggagat cgtgaaggac cggaagggga gctgcccgat ctgcggcatg
     6001 gacctcgtga ggaaggagga gggggattcg gcgggcggcg caggtgtggc gggggtgccc
     6061 ggcttcgcga ccgtgacgat cgacgcccgc aagcggcagc tcctctcgct gaagaccgcg
     6121 aaggtcgagc gggggacgtt cggcaccggg atccggacgg tcggccgcgt cgcgttcgac
     6181 gagcggcgcg tccaccacgt ccacacgcgc tacgaggcct acgtcgagca cgtcgccgcc
     6241 gacttcacgg ggaagcacgt gaggagggga gaggtcctcg cccacgtcta cagccccgag
     6301 cttttcgcga cccagcagga ggtcctcctc gccctgcgcg ccgtccgcgc gctcgggccc
     6361 caggccgacc cgtcggcccg cgaaggggcg gagcggctcc tcgaggcggc gcggcagcgc
     6421 ctccgcctct gggaggtgac ggagaaggac atcgccgcgc tcgaggcccg gggcgagccg
     6481 cttcggagcg tcccgatcta cgcgccgtcc tcgggttacg tgacggcgcg gacggccttc
     6541 cacggcatga aggtgatgcc ggccgacacg ctcttcgaca tcgtcgacct ctcgtccgtc
     6601 tgggtcctcg ccgacgtcta cgagtcggag ctgccnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnngccgt cctcgacagc gggacgcgga aggtcgtctt cgtcgcgaag
     6781 ggagaagggg tcctcgagcc gcgggaggtg aaggtcggcg agcagggggg cggggcgtgg
     6841 gagatcctct cgggcctcgc ggaaggggag gagatcgccc tcggggcgag cttcctcgtc
     6901 gactccgagt cgcgcctcgc cgcggccctc gccgggatga gggacggcgc gactccggcg
     6961 acgggtcccg aaggccacgt ggccgcaccc gcgcccccgc catcggctcc tcacgacggg
     7021 cacgagcact gagggggccg tcgtgatccg gaagatcatc cgcttctcgg cggagaacaa
     7081 gtacctcgtt ctcgccctct gggcggtcgc ggtcctcctt gcgttccgcg cgctccggac
     7141 cgtcccgctc gacgcgatcc ccgacctctc cgacacccag gtcatcgtcc tctcgaggtg
     7201 ggaccggagc ccggacatcc tcgaggacca ggtgacctac ccgatcgtca ccgccctcct
     7261 cggggcgccg aaggtgaaga ccatccgggg cttctccgac ttcggctact cgttcgtcta
     7321 cgtcatcttc gaggacggga cggacctcta ctgggcccgc tcgcgagtcc tcgagtacct
     7381 ctccaagatc caggggagcc tgccgtcggg cgtgaagacg gagatcggcc ccgacgcgac
     7441 cggcgtcggg tgggtctacc agtacgctct cgtcgacgag acggggaaga cctccctcga
     7501 cgagctccgc tcctaccagg actggttcct tcgctaccag ctccagtcgg tgcccggggt
     7561 cgcggaggtc gcgaccgtcg gcgggatggc gcggcagtac caggtcaccg tcgacccgaa
     7621 tcgcctcgcg gcctacgcca tctccctgga ggccgtgtcg gacgcgctcc agaggagcaa
     7681 cgacgaggtc ggcggccggc tcatcgagat ctccggccgc gagtacatgg tgcgggggcg
     7741 cgggtacctc aagggagcag cggacatcga gaaggtcgtc gtgaagaccg acgagcgcgg
     7801 gacgccggtg acgatcaagg acgtcgggag cgtcgccgtc gggcccgacc tgcgccgcgg
     7861 gctcgccgag ctggacggga agggggagac cgtcggcggc atcgtcgtca tgcgctccgg
     7921 ggagaacgcc ctccacgtca tcgagcgggt gaaggagcgg atcgacgagc tccggccctc
     7981 gctcccgccc ggggtggagc tcgtgacgac ctacgaccgc tcgacgctca tcgagtcggc
     8041 gatcgacaac ctgaagggga agctggtcga ggagatcgtc atcgtctcga tcgtcatcct
     8101 catcttcctc tggcacgtcc cgagcgcgct cgtcccgatc ctgacgatcc ccgtgtcggt
     8161 cctcctcgcg ttcatcccgc tccacctgat ggggatcacg gtcaacatca tgtcgctcgg
     8221 cggcatcgcg atctccatcg gcgtcctcgt cgacggcgcg atcgtcgagg tggagaacgc
     8281 ctacaagaag ctccagatct ggaacgactc ggggcggaag ggggatttcc acgccgtccg
     8341 cctcgaggcg ctgatggagg tcgggccgtc ggtcttcttc tccctcctcg tcatcgccgt
     8401 ctcgttcctc ccgatcttca cgctcgtcga ccaggagggg cgcctcttca cgccgctcgc
     8461 ctggtcgaag aacctcgcga tggcgctcgc ggccgtcctc gcgatcacgc tcgacccggc
     8521 gatgcggatg ctcttcgcgc ggatggaccc gttccggttc ggcttcgcgc acgacgggca
     8581 gggcgcgctg gcgcggctcg tacgccttcc tgcccgcctc gtcgagaagg ccgtcaacgc
     8641 cgtcgccgtc gggacgtacc acgccgagga gaggcacccc gtctcgaagg tcctcttccg
     8701 gctctacgac cccgtctgcc gggccgtcct ccgccacccg aagaaggtcg tcgcggtctc
     8761 gctcctcgtc ctcgtctcga ccgtgccggt ctacctctcc ctcgggtcgg agttcatgcc
     8821 gccgctccac gaggggacgg tcctctacat gccgacgacg ctccccggca tctcggtcac
     8881 cgaggccgcg aagctcctcc agacgcagga ccgggtcctc gcgacgttcc ccgaggtgga
     8941 gcgggtcttc ggcaaggcgg ggcgcgcgga gacctcgacc gacccggcgc ccctctcgat
     9001 gatggagacg acggtcatcc tga
//
LOCUS       DAOTEZ010000156         8924 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5541_length_8924_cov_5.123577, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000156 DAOTEZ010000000
VERSION     DAOTEZ010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8924
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5541_length_8924_cov_5.123577"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="P5164_09630"
     CDS             <1..242
                     /locus_tag="P5164_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44193.1"
                     /translation="GLFSLWSFGYKMYRYGHDLDPKAAMNVDPFMPPLFGGKQLANFE
                     VFSYPQAGSYTLAVAMVFLALAFYFGWRESRKAAS"
     gene            260..1642
                     /gene="nosD"
                     /locus_tag="P5164_09635"
     CDS             260..1642
                     /gene="nosD"
                     /locus_tag="P5164_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HRY44194.1"
                     /translation="MRLPAAAALLLALALPARAEEMTPVLPGALEGRPAPRDTSPIQK
                     LVDAAAPGATVEVPAGTYEGDLVLDRPVKLVGRGRPLLLGSRSGSVVRVRAPNVTVEG
                     FDIDGREGGDLGKDSAGVHTSAPGTTVRSCRVRGALFGVYVREANDAVVEDSEIRGIP
                     GRSPGEKGSGIHIWNTVGFRFLRNVIVDVRDGFYIQSSSHGLVAGNRASDLRYGIHYM
                     FSDDNVFEDNVFENGAAGSALMYSKRIAFRRNQFLRNRGFASVGLLFKTCDDVVAEGN
                     LLADNARGVFFEDSYRNVFRGNVVASSDTAIVLFAGCGQNRITGNSFVGNLTPLTLVG
                     KTTDTVFDGNYWSENREPDLDGDGRSDRPYRLGNVFDHFRGNLLAADLFAQSFAAGAI
                     GVAEETFPVLRQQMAMDDSPLARPPALPDVPRAKPAVRVANVPAVAASAATFLFGTAL
                     LVLGRRLPAA"
     gene            1656..2549
                     /locus_tag="P5164_09640"
     CDS             1656..2549
                     /locus_tag="P5164_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY44195.1"
                     /translation="MIRFDAFGKSYGTQVAVRDVSLEVGAGEVVALLGPNGSGKTTCL
                     KAVAGLLHATSGRVLVDGRPASEPAARRGLSFLPQKVAFPDALTGREVVEFYRRLRGV
                     PAPRTAEVLRFACLNGAAARAVSTYSGGMVQRLGLAVAALPDTPLLLLDEPTAALDPE
                     GLAAFYALVEGRRREGRTILFTSHHLGDVERLADRFAVLVEGRLAALLSAAELAHRLA
                     DRGLLRLRLDRAPGAVLGSIRAACPGAAATEEELLLPGPASARPAYLEAVRATGAAVL
                     ALSSDEGRLDALYTELVGGPR"
     gene            2546..2998
                     /locus_tag="P5164_09645"
     CDS             2546..2998
                     /locus_tag="P5164_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HRY44196.1"
                     /translation="MRSPLRVVAVLSVAVLAACGGGADGPVPVDTKNDACAWCRMSVS
                     DLRFAAQLTAPGHEPKLFDDAGCLRDWLKEPREAAEWTAWLSDHRTREWVKATDAVVV
                     REPAIQTPMDSGLVAWANAASREQDPTGKSGSPVPIAEVIGPFAAGAK"
     gene            2995..3831
                     /locus_tag="P5164_09650"
     CDS             2995..3831
                     /locus_tag="P5164_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY44197.1"
                     /translation="MSGSPFVLCARQELLLAIRSRWTQIFAVVFAGLALAVAVAGYAL
                     SGGHGVQDFARTSASLVQLVLLLVPLSSLLIGVLSLAPERGAAELLFSQPVSRTSILL
                     GQSTGLWAALTAAQAIGFGAAGTIVFSHRGSDGLGGFVLLFAGSSLLTAVFLGLAALL
                     AAGAIGRRRTRALALALVVWFVAVVLLDVVALGVASVLKSGTASRVLILSVIVNPVGA
                     LRTGALLALQGTTAFGSASLAFLRFTGGTTWAFVLLSGSLVAWAAIPFVLAARRLSRT
                     DL"
     gene            3905..6475
                     /locus_tag="P5164_09655"
     CDS             3905..6475
                     /locus_tag="P5164_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44198.1"
                     /translation="MKYAPLAAAAAAAWLLLSSTAVASGTAYRSVLYPVAGNEGSSGD
                     REAGANGAFWIPSSAYDNPTGGWKPIVHRLDPSGGVATWALDPVARSFFPVAVRWHSA
                     GGRAIVLVPLGAEQRALVYALDPATGGATRTEIPFLARSLRLDPQDGSTWLVGRDTVA
                     RLSGGTLRAWTIPGVVDAAGRFDAGDRLWLATEGGVLLSMTRDGAVASWGPGSSPGSL
                     LTVDESGTELWGVDGARDRLVRFRPSTGERTAWPQVRPVSNWAGLAVSGGTVALAASY
                     GPHVVSATVADLGPGTTTTSTGSPVQTDAPADGMAGPAPVTPVRTDSTVTRSARALYA
                     PLDADRALFTTSGEAYRALLWAGAGTFLVATDGVELWTPLAADAPFATRQVLPVAVEV
                     RPDDPTRNFFTEVTVANLDAERDVLLTLTTSTAVHALAIDLPAGTTRVFPNVVETFRD
                     LGLAYLPPGETVAGTLAAEFRSGSGSLAARVYTRFPDGSTTGLGFTSLDAAREARVFR
                     KTLNGLKNTAGFRTNVAVANLCGLEGPCATLDLSADFFDDATGTAVGTATLQVPPGQW
                     RQLDAPLTRFAGATGETFSVVFVPFSTGATAYDAYATVVSNANQDAAFVRATPSERAS
                     NLSLPVITDAGGIGTRFTSELALTNTTGAATTADVTFTSAKSGNVVSETLQLASGLGV
                     LWPNAVGHFRSISPASVEADDYGPVRIAFRDFASGFASVRTTASNGTGLGFTAIDPFV
                     ERAKRTKRIAGLVESARFRTNLAIVHAGATGTDPSAQITLRVTVRDAAGIVVGAPLER
                     TLGPGQLHQWTKVLSDSLGRTGEGFTATIERIDGFDPFDAYVTVIDNASTDPVFVRAE
                     "
     gene            complement(6485..7012)
                     /locus_tag="P5164_09660"
     CDS             complement(6485..7012)
                     /locus_tag="P5164_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HRY44199.1"
                     /translation="MSGLSITPEAKVGELLEAWPELEATLVAIAPAFAKLRNPILRRT
                     VARVATLAQAARVGGVDVRTLVGTLRQAAGLPGEPTAAEAAAAGEVLAAPPAWHDEAR
                     VVARIDADEVLARGDHPLGAVQGAAASLPKGGELLLLSSFLPAPLVDAMRKKGFETAS
                     FRADGGRVATLLRLP"
     gene            complement(7009..8247)
                     /locus_tag="P5164_09665"
     CDS             complement(7009..8247)
                     /locus_tag="P5164_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HRY44200.1"
                     /translation="MSELINNRAKRIEELKGIIRHLHAGASPEEVRGQLRQIVRETDA
                     TEIAAMEQSLIADGMPVEEVRSMCDLHSQVLREFVLPAPTVGDAPPGHPVDTFRRENE
                     AILAAVARLRESLAALAGNGAAEARDRARAVAHELFDVEKHYRRKEELLFPFLEKHGI
                     VGPSKVMWAKDDEARGLLKALSEALSVEEASAEEWRLVVETVGEPAAAALAEMVSKEE
                     LVLLPLSLGTLSADEWGLVHAESPRVGWCLVPPREGWLPPLPSFPPDALSLPAGKAVA
                     LPSGHLTLEQLQGIFEALPVDLTFVDADDRVAFFSEGPDRVFSRTRAILGRKVQHCHP
                     PKSVDVVERILDDFRAGRQHVAEFWLELHGRFVHVRYFAVRDAKGVYAGCLEVTQDAT
                     RIRALRGERRLLQYETAVTA"
     gene            complement(8406..>8924)
                     /locus_tag="P5164_09670"
     CDS             complement(8406..>8924)
                     /locus_tag="P5164_09670"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRY44201.1"
                     /translation="ALGTLKIVKATPTRSVILRVVGAGSPVGIVAAWEGRPYPGTAIA
                     VEPSTVVHVPEREFFALSDRHPEILRQLLQLMMARQVQLGQRVSEMTGQVETRIAQAL
                     LDLVTSSGKVEGTKAVIHLPLTRQEIADLAGTTVETAIRTMSRWGKEGIVVTGEEGFL
                     IPDVGRLRALVG"
BASE COUNT         1110 a         3440 c         3042 g         1332 t
ORIGIN      
        1 tcgggctctt ctcgctctgg tccttcggct acaagatgta ccgctacggg cacgatctcg
       61 acccgaaggc ggcgatgaac gtcgatccgt tcatgccccc gctcttcggc ggcaagcagc
      121 tcgcgaactt cgaggtcttc tcgtatcccc aggccggctc ctacaccctg gccgtggcga
      181 tggtcttcct cgccctcgcg ttctacttcg ggtggcgcga gtcgcggaag gcagcttcct
      241 gacgtgcgcg aagcctgacg tgagacttcc ggcggccgcc gcgctcctcc tcgcgctcgc
      301 ccttccggcg cgcgccgagg agatgacccc ggtccttccg ggcgccctcg aggggcgtcc
      361 cgcgccgcgc gacacctcgc cgatccagaa gctcgtcgac gcggccgccc cgggcgcgac
      421 ggtcgaggtc ccggcgggaa cgtacgaggg cgacctcgtc ctcgaccgcc ccgtgaagct
      481 cgtcggccgc gggcgccccc tcctcctcgg ctcccggtcc gggagcgtcg tccgcgtgag
      541 ggccccgaac gtcaccgtcg aggggttcga catcgacggg cgggaagggg gcgacctcgg
      601 gaaggactcc gcgggggtcc acacgtccgc accggggacg acggtccgtt cctgccgggt
      661 gcgcggagcg ctcttcgggg tctacgtccg cgaggcgaac gatgccgtcg tggaggacag
      721 cgagatccgc ggcatcccgg gcaggtctcc cggcgagaag ggctccggca tccacatctg
      781 gaacacggtc ggcttccggt tcctgcgaaa cgtcatcgtc gacgtccggg acggcttcta
      841 catccagtcc tcgagccacg ggctcgtggc cggcaatcgc gcctcggacc tgcgctacgg
      901 gatccactac atgttctcgg acgacaacgt cttcgaggac aacgtcttcg agaacggggc
      961 cgcggggagc gctctcatgt actcgaagcg gatcgcgttc cggcggaacc agttcctcag
     1021 gaatcgcggc ttcgcttccg tcggcctcct cttcaagacc tgcgacgacg tcgtcgccga
     1081 ggggaacctc ctcgccgaca acgcccgcgg cgtcttcttc gaggactcct accggaacgt
     1141 cttccgcggg aacgtcgtgg catcgtccga cacggcgatc gtccttttcg ccgggtgcgg
     1201 gcagaaccgg atcacgggga actccttcgt cggaaacctc acgccgctga cgctcgtcgg
     1261 gaagacgacg gacaccgtct tcgacggcaa ctactggtcc gagaaccgcg agcccgacct
     1321 cgacggcgac ggacgctccg accggcccta ccgcctcggg aacgtcttcg accacttccg
     1381 cggaaacctc ctcgcggcag acctcttcgc ccagagcttc gcggccgggg cgatcggcgt
     1441 ggcggaggag accttccccg tactgcgcca gcagatggcg atggacgact ctcccctcgc
     1501 ccgcccgccg gccctccccg acgttccacg tgcgaaaccc gccgtgcgcg tcgcgaacgt
     1561 tccggccgtc gcggcctccg ccgccacgtt cctcttcggc accgcgctcc tcgtcctggg
     1621 ccggcgtctc ccggcggcat gagagagtgg aatcgatgat ccgcttcgac gccttcggaa
     1681 agagctacgg cacccaggtc gccgtccgcg acgtctcgct cgaggtcggg gccggcgagg
     1741 tcgtagcgct cctcgggccg aacggctcgg gaaagacgac ctgcctcaag gcggtcgcgg
     1801 gcctcctcca cgcgacctcg gggcgggtcc tcgtcgacgg acgcccggcc tcggagccgg
     1861 ccgcccgccg gggcctttcg ttccttcccc agaaggtcgc cttccccgac gccctgacgg
     1921 ggcgcgaggt cgtggagttc taccggcgcc ttcgcggcgt tcccgcgcct cggaccgccg
     1981 aggtcctccg attcgcgtgc ctgaacgggg ccgcagcgcg cgccgtgtcg acctactcgg
     2041 ggggaatggt ccagcgcctc ggcctcgccg tcgccgcgct ccccgacacg ccgctcctcc
     2101 tcctcgacga gccgaccgcc gccctcgacc cggaaggtct tgccgcgttc tacgccctcg
     2161 tcgaggggcg ccgccgcgag gggaggacga tcctcttcac gtcgcaccac ctcggcgacg
     2221 tcgagcgact cgccgaccgt ttcgccgtcc tcgtggaggg gcggctcgcc gcgctcctct
     2281 cggcggcgga gctcgcgcac cggctcgccg acaggggcct cctccgcctc cggctcgacc
     2341 gcgcccccgg cgcggtcctc ggctcgatcc gtgccgcgtg cccgggggcc gcggcgacgg
     2401 aggaagagct ccttctcccg ggtccggcat ccgcccgccc ggcgtacctc gaggcggttc
     2461 gcgcgaccgg cgcggcggtc ctcgctctct cttccgacga ggggcgcctc gacgccctct
     2521 acaccgagct cgtgggaggt ccgcgatgag atccccgctc cgggtcgtcg ccgtgctgtc
     2581 ggtcgccgtc ctcgccgcct gcgggggcgg cgccgatggt cccgtccccg tcgacacgaa
     2641 gaacgacgcc tgcgcctggt gccggatgtc ggtctccgac ctccgcttcg ccgcccagct
     2701 gacggctccc ggccacgagc cgaagctctt cgacgacgcg ggctgcctgc gcgactggct
     2761 gaaggagccg cgggaggccg cggagtggac cgcctggctc tccgaccacc ggacaaggga
     2821 gtgggtgaag gcgacggacg ccgtggtcgt gcgcgaaccg gcgattcaga cgccgatgga
     2881 ctccgggctc gtcgcctggg cgaacgccgc ctcgcgcgag caggacccga caggaaagag
     2941 cggctcgccc gttccgatcg ccgaggtgat cggcccgttc gccgcgggag cgaaatgagc
     3001 ggctccccgt tcgtcctttg cgcccggcag gagctcctcc tcgcgatccg ctcccggtgg
     3061 acccagatct tcgccgtcgt cttcgccggc ctcgcgctcg ccgtcgccgt cgcgggctac
     3121 gccctctccg gcggacacgg cgtccaggac ttcgcccgga cgtcggcctc gctcgtccag
     3181 ctcgtcctcc tcctcgtccc gctctcctcg ctcctcatcg gcgtcctctc gctcgcgccg
     3241 gagcggggcg cggccgagct cctcttctcg cagcccgtgt cgcgaacgtc gatccttctc
     3301 gggcagtcga ccggcctctg ggccgccctg acggcggccc aggcgatcgg gttcggggcg
     3361 gccggcacga tcgtcttctc ccaccgtggg agcgacggac tcggcggctt cgtcctcctc
     3421 ttcgccggat cgtccctgct gacggccgtc ttcctcggcc tcgccgcgct cctcgcggcc
     3481 ggggcgatcg ggcgacgcag gacgcgcgcc ctcgcgctcg ctctcgtcgt ctggttcgtc
     3541 gcggtcgtcc ttctcgacgt cgtcgcgctc ggcgtcgcgt cggtcctcaa gtcggggacc
     3601 gcctcgcggg tcctgatcct gagcgtgatc gtgaatcccg tcggagccct gcgcaccggc
     3661 gctctcctcg cgctccaggg gacgacggcg ttcggctcgg cctcgctcgc gttcctccgt
     3721 ttcacgggcg ggacgacctg ggccttcgtc ctcctctccg gatcgctcgt cgcctgggcc
     3781 gcgatcccgt tcgtgctcgc cgcgcggaga ctctcccgga ccgacctctg atcctcgagc
     3841 tgcggcgacg ccgccgcctc gacggccgcc cggttcgcgg ctatcgtccc gggcggaggc
     3901 ctccatgaag tacgcacctc tcgcggccgc cgcggcggcg gcctggctcc tgctttcgag
     3961 caccgccgtc gcctcgggaa ccgcctacag gagcgtcctc tacccggtcg cggggaacga
     4021 gggctcctcc ggcgaccgcg aagcgggggc gaacggcgcg ttctggattc cctcctcggc
     4081 ctacgacaac cccaccggcg gctggaagcc gatcgtccac cggctcgacc cgtccggcgg
     4141 cgtcgcgacg tgggcgctcg acccggtggc ccggtcgttc ttcccggtgg ccgttcggtg
     4201 gcattccgcg ggcggccggg cgatcgtcct cgttccgctc ggggcggagc agagggccct
     4261 cgtctacgcg ctcgatccgg cgaccggcgg cgcgacgcgg acagagatcc cgttcctcgc
     4321 gcggtccctt cgcctcgacc cgcaggacgg ctcgacctgg ctcgtcgggc gggacacggt
     4381 cgcccggctc tcggggggca cgcttcgcgc ctggacgatc cccggcgtcg tcgacgccgc
     4441 cggccgtttc gacgcgggcg accggctctg gctcgcgacc gagggaggcg tcctcctctc
     4501 gatgacgagg gacggggccg tggcaagctg gggtcccggc tcgtctccgg gatcgctcct
     4561 caccgtcgac gagagcggta ccgagctctg gggcgtcgac ggggcgcgcg accggctcgt
     4621 ccgcttccgc ccgtcgaccg gcgagaggac cgcctggccc caggtccggc ccgtctcgaa
     4681 ctgggcgggc ctggcggtct ccggcgggac cgtcgcgctc gccgcttcgt acggtccgca
     4741 cgtcgtctcg gcgaccgtcg cggacctcgg ccccggaacg accaccactt cgaccgggag
     4801 cccggtccag accgacgcgc cggccgacgg catggccggg cccgcgcccg tgacgcccgt
     4861 ccggaccgat tcgaccgtca cgcggtctgc gcgggccctc tacgcgccgc tcgacgccga
     4921 ccgagcgctc ttcacgacga gcggcgaggc gtaccgcgcc ctcctctggg ccggcgccgg
     4981 cacgttcctc gtcgccacgg acggggtcga gctctggacg ccgctcgcgg ccgatgcgcc
     5041 gttcgcgacg cgccaggtcc tccccgtcgc ggtcgaggtc cggccggacg acccgacccg
     5101 gaacttcttc accgaggtga cggtggcgaa cctcgacgcg gagcgggacg tcctcctcac
     5161 gctgaccacg tcgaccgcgg tccacgcgct cgcgatcgac ctccccgcgg ggacgacgcg
     5221 cgtcttcccg aacgtcgtcg agacgttccg cgacctcggt ctggcctatc tcccgccggg
     5281 cgagaccgtg gccgggacgc tcgcggccga gttccggagc ggcagcggga gcctcgcggc
     5341 gcgcgtctac actcgcttcc ccgacggctc gacgaccggg ctcgggttca cctccctcga
     5401 cgcggcgcgc gaggcacgcg tcttccggaa gacgctgaac ggcctgaaga acaccgccgg
     5461 gttccggacg aacgtggcag tcgcgaacct ctgcggcctc gaggggccgt gcgcgacgct
     5521 cgacctttcc gccgacttct tcgacgacgc caccgggacc gcggtcggca cggcgacgct
     5581 ccaggtgccg ccggggcagt ggagacagct cgacgcgccg ctcacccgct tcgcgggcgc
     5641 cacgggcgag acgttctccg tcgtcttcgt cccgttctcg acgggcgcga cggcctacga
     5701 cgcctacgcc acggtcgtct cgaacgcgaa ccaggacgcc gcgttcgtac gggccacgcc
     5761 gtcggagcgg gcctcgaacc tgtcgctccc cgtgatcacc gacgcgggcg ggatcgggac
     5821 ccgcttcacc tccgagctcg cgctcacgaa cacgaccgga gccgcgacga cggccgacgt
     5881 gacgttcacg tcggcgaagt ccggaaacgt cgtctccgag acgctccagc tcgcctccgg
     5941 cctcggcgtc ctctggccga acgccgtcgg ccacttccgc tcgatctccc ccgcctccgt
     6001 cgaggccgac gactacggcc cggtgcggat cgcgttccgc gacttcgcga gcgggttcgc
     6061 ctcggtgcgg accaccgcct cgaacggcac cgggctcggc ttcaccgcga tcgacccgtt
     6121 cgtcgagcgg gcgaagcgga cgaagcggat cgccggcctc gtcgagagcg cccggttccg
     6181 gacgaacctc gccatcgtcc acgccggcgc gacggggacc gacccgtcgg cgcagatcac
     6241 cctgcgcgtc accgtgcgcg acgccgcggg gatcgtcgtc ggcgccccgc tcgagcgaac
     6301 gctcggcccg ggccagctcc accagtggac gaaggtgctg tccgactccc tcgggcggac
     6361 gggcgagggc ttcacggcga cgatcgagcg gatcgacggg ttcgacccgt tcgacgccta
     6421 cgtcaccgtc atcgacaacg cctcgaccga cccggtcttc gtgagggcgg agtagcgccg
     6481 cgccctacgg cagccggagg agcgtcgcga ccctcccgcc gtcggcgcgg aagctcgccg
     6541 tctcgaaccc cttcttccgc atcgcgtcga cgagcggcgc gggaaggaag ctcgagagaa
     6601 ggagcagctc gccccctttc gggagcgacg cggccgcccc ctgcacggcg ccgagggggt
     6661 ggtctcccct cgcgaggacc tcgtcggcgt cgatccgggc gacgacccga gcctcgtcgt
     6721 gccaggccgg cggcgcggcg aggacctcgc ccgccgccgc ggcctccgcg gcggtcggct
     6781 cccccgggag gcccgccgcc tgccggagcg tcccaacgag agtccggacg tccactcccc
     6841 cgacgcgggc cgcctgggcg agcgtcgcga cgcgcgcgac ggtcctccgg aggatcgggt
     6901 tcctcagctt cgcgaaggcc ggggcgatcg cgacgagcgt cgcctccagc tccggccagg
     6961 cctcgaggag ctcccccacc ttcgcttccg gggtgatcga aaggccgctc acgccgtcac
     7021 cgccgtctcg tactggagga gccgccgctc gccgcggagc gcgcggatcc gggtcgcgtc
     7081 ctgcgtgacc tcgagacatc ccgcgtagac acccttcgcg tcgcggaccg cgaagtagcg
     7141 gacgtggacg aagcgcccgt ggagctcgag ccagaactcg gcgacgtgct gccgcccggc
     7201 acggaagtcg tcgaggatcc gctcgacgac gtcgacgctc ttcggcgggt ggcagtgctg
     7261 caccttccgg ccgaggatcg cgcgggtccg cgagaagacc cggtcgggcc cctcgctgaa
     7321 gaaggcgacg cggtcgtcgg cgtcgacgaa cgtcaggtcg accgggagcg cctcgaagat
     7381 cccctggagc tgctcgagcg tcaggtgccc ggacggcagg gcgaccgcct tcccggccgg
     7441 gagcgacagc gcgtccggcg gaaagctcgg caggggcggg agccacccct cccgcggcgg
     7501 gacgagacac cagccgacgc gcggcgactc ggcgtggacg agcccccact cgtcggccga
     7561 gagcgtcccg agggagagcg gaaggaggac gagctcctcc ttggagacca tctccgcgag
     7621 cgccgccgcc gccggctcgc cgaccgtctc gacgacgagc cgccactcct ccgccgacgc
     7681 ctcctcgacc gagagcgcct ccgagagcgc cttcaggagg ccgcgcgcct cgtcgtcctt
     7741 cgcccacatc accttcgacg gaccgacgat cccgtgcttc tcgaggaacg ggaagaggag
     7801 ctcctccttc ctccggtagt gcttctccac gtcgaagagc tcgtgcgcca cggcccgcgc
     7861 ccggtcgcga gcttccgccg ccccgttccc ggcgagcgcg gcgagcgact cgcggagcct
     7921 cgcgaccgct gcgaggatcg cctcgttctc gcggcggaag gtgtcgaccg ggtgacccgg
     7981 cggagcgtct ccgaccgtcg gcgccgggag gacgaactcc cggaggacct gcgagtggag
     8041 gtcgcacatc gagcggacct cctcgacagg catcccgtcg gcgatgagcg attgctccat
     8101 tgccgcgatc tccgtcgcgt cggtttcccg gacgatctga cggagctgcc cgcgcacctc
     8161 ttcgggtgac gctccggcgt gcaggtgccg gatgatcccc ttcagctcct cgatccgctt
     8221 cgcgcggttg ttgatcagct cgctcatcgg gtcctcccgt tcacgggcgg aaggatcgcg
     8281 ggcctcgtcg ccggcgcgtt atgacgtccg tcagtcaagt cgagatatcg tcgcaggacg
     8341 gcgggctcgc cctcgacctc tccagcgacg cagctccgga ggcggatggc tctcggccgc
     8401 cgggttcagc ccacgagcgc ccggagccgc ccgacgtccg gaatcaggaa cccttcctcc
     8461 cccgtgacga cgatcccctc cttgccccag cggctcatcg tccggatcgc cgtctcgacc
     8521 gtcgtcccgg cgaggtccgc gatctcctgc cgcgtcaggg ggaggtggat caccgccttc
     8581 gttccctcca cctttcccga ggaggtgacg aggtcgagca gggcctgcgc gatgcgcgtc
     8641 tcgacctggc cggtcatctc ggagacgcgc tgcccgagct gcacctgccg ggccatcatc
     8701 agctggagga gctggcggag gatctcaggg tgccggtcgg agagggcgaa gaactccctc
     8761 tcgggcacgt ggacgaccgt cgagggctcg acggcgatcg ccgtgcccgg gtagggccgc
     8821 ccctcccacg ccgcgacgat cccgaccggg cttccggccc cgacaacccg gaggatcacg
     8881 ctccgggtcg gggtcgcctt gacgatcttc agcgtgccga gggc
//
LOCUS       DAOTEZ010000157         8864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5597_length_8864_cov_5.562833, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000157 DAOTEZ010000000
VERSION     DAOTEZ010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8864
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5597_length_8864_cov_5.562833"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="P5164_09675"
     CDS             <1..666
                     /locus_tag="P5164_09675"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HRY44202.1"
                     /translation="WDQNAGCSGWLYGLHIADGLIQAGKARNVLLVGAEFLTRYVDYS
                     DRATCVLFGDGAGATILRATKSDRGVLASTIRSDGEGACFIQMPGGGSSFPPTRPETL
                     EQRLPFIKMMGGETFKIAVRSMVEVCKDVLNESGFEPGEVSWLVPHQANDRIIQAVGE
                     RLGIPRERCIVNIERYGNTSAASIPIALDEAARDGRVKRGDLILFTAFGAGLTWGAAL
                     VRW"
     gene            669..1607
                     /gene="fabD"
                     /locus_tag="P5164_09680"
     CDS             669..1607
                     /gene="fabD"
                     /locus_tag="P5164_09680"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRY44203.1"
                     /translation="MASLSFLFSGQGSQYAGMGRDLVAAHPEARAVYERADAALAPFG
                     LAISRVSFEGTDEELKRTAVTQPAILVHSVAVFEVLKARGLSPTMLAGHSLGEWSALV
                     AAGALSLEEAVVLVHRRGTFMQEAVPAGEGAMGAVIGLDGALVAEACAEVEAATGEAV
                     SAANFNSPEQTVIAGTTNAVAKAGELLRQKGAKRVLPLPVSAPFHCALMKPAEEKLGP
                     FLEAAPFSDLAVPVVTNVDVLANLSGAEAREALRRQVCSPVRWVETVQRLSRDTEGAI
                     EVGPGTVLAGLAKRIAKDWPVRTTSDAEGIGKLLAG"
     gene            1604..2080
                     /locus_tag="P5164_09685"
     CDS             1604..2080
                     /locus_tag="P5164_09685"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HRY44204.1"
                     /translation="MTTKTTTNATRVLDARKAVYALRTFAAADFTAEEAAEKLGVPPG
                     EVWKTLVAKGDRTGLLAAVIPTGTRLSLKKLARASGNRTVAMVSPEEMEKATGYVKGG
                     CSPFGMRKAMPLYVHEAAAALPRLFVSAGQRGVQLEMAGGALLAASGAAATDLVEE"
     gene            2103..2849
                     /gene="fabG"
                     /locus_tag="P5164_09690"
     CDS             2103..2849
                     /gene="fabG"
                     /locus_tag="P5164_09690"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005035360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HRY44205.1"
                     /translation="MTLEGKVALVTGASQGIGEAVARRLAAAGATVLAAARSEEKAAA
                     VAASIAEAGGAAESIRLDVSDPASVTAAFKGIVERHGKLDVLVNNAGITDDGLLLRMS
                     KESWDRVIATDLTGVFLVSQEAVKLMMKKRVAGRIVTVGSVVGLMGNPGQANYAAAKA
                     GVVGFTMSLAREIGSRGITVNAVAPGYVETAMTGALSDDQRKSLAEKIVLGRLGTGED
                     VAGAVLYLASDLASYVTGTVLNVSGGLLIP"
     gene            2946..3179
                     /locus_tag="P5164_09695"
     CDS             2946..3179
                     /locus_tag="P5164_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020560661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRY44206.1"
                     /translation="MEDKVKSIIVEQLGVQAEEVKPEASFVDDLGADSLDTVELVMAF
                     EEAFGIEIPDEDAEKIQKVKDAVTYIESHAKPA"
     gene            3198..4454
                     /gene="fabF"
                     /locus_tag="P5164_09700"
     CDS             3198..4454
                     /gene="fabF"
                     /locus_tag="P5164_09700"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRY44207.1"
                     /translation="MDHLDARGRRRVVVTGIGLVSPVGLTREENWGALLAGRSGIGPI
                     TRFDASDYACRIAGEVKGFDPALFLDRKEIKKFDTFVHYAVAAAKEALAESGLGITEE
                     NAEAVGVCVGSGIGGLPLIEETHKVLLEKGPRRISPFFIPGLIANMSSGLISILTGAK
                     GPNTATVTACATSAHAIGDAVNIIRRGDADAMISGGTEAVVSPLAVGGFAAMRALSTR
                     NDDPAAASRPWDRDRDGFVLGEGAGILVLEEMGNAIRRGAAIYAEVAGYGMSGDAHHI
                     SAPSDDGDGPFRVMRNALKDAAMEPGEVGYVNAHGTSTPHGDRIETIALKRLFGDGAR
                     NVPVSSTKSMTGHLLGAAGGYEAGVLALAIRRGVLPPTVNYTTPDPECDLDYTPNVAR
                     ETRVRAGLSNSFGFGGTNACLVMRAV"
     gene            4549..6045
                     /locus_tag="P5164_09705"
     CDS             4549..6045
                     /locus_tag="P5164_09705"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRY44208.1"
                     /translation="MKADLIRRKFFDGVPRYEWIETLGQGGVGIVYKAKDLELDEVVA
                     IKVLSPDIAKEDEVVLARFKREINLNRKIKHPNVARMYDFGISGEYPYITMEVVPGKD
                     LWTMIRDEGRLPPPKAVPILRQIARGCSAIHELGIVHRDLKSQNVVVDEQGAVAIVDF
                     GLARWNLGGGFTLDSIILGTPQYMSPEQASGKTVDARTDIYSIGIIAFEALTGQLPFT
                     GDSPIAVAMMQINDPVPDLLPKFADVSPDLREVVLKALRKDPAKRYATAVDLEADLAS
                     AERLPTPREGALARPVGGDPLMAQLESVLGAVILPAPPEKPRRPSPSDASAPVTRPSV
                     VPRPAAAKPAPTPAKPAPPMAPARPPTVLIVSSAPAERAAWKAGLGIGPCRVLEAGSG
                     PEALEALVREPADLVLMAVALPGMDGFDVTRVLKSQPNLAALPVLLASDRLERSHYAF
                     GIQSGANELLVKPIEPVRLREAVRGLLRHRGFPLPDGPADAEPGAGNG"
     gene            6098..7015
                     /locus_tag="P5164_09710"
     CDS             6098..7015
                     /locus_tag="P5164_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44209.1"
                     /translation="MPSESEPDDPVGEAEASAERVRVDERRAGYWDPTASGRHQISRP
                     IAIDGLRAERARIELAPRVVRRGATYYDDSYHVVDAAARQLASIFTLEQLVMATVALR
                     VHMRRGREHIGRKKELQERWTVLAHRLMKLRAEERHLALREQRITEEHASLAGADSER
                     PGRAELLALEEAEVAQARQSWRDESRRAWEAWTRLESEEGALLAGAPGLEQTEDLKQP
                     YILLLVAARRAAPRSVAAAMHTLVDALQRPLSRTDQMLLEIAFRRCIRAGSPEDERDL
                     ERWEHALLADAEREFASEFPWARPYLGNG"
     gene            7074..7880
                     /locus_tag="P5164_09715"
     CDS             7074..7880
                     /locus_tag="P5164_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HRY44210.1"
                     /translation="MCLIVLALHAHARHPLVVAANRDEDLDRPTAPAAFWPGEAGLLA
                     GRDLRSGGTWMGVTRTGRFSALTNVRDPRAHDPVAPSRGGLVVRFLAGGAEPLAHARA
                     VAAERARANGFNLLAGAGGRLAWVSNAGGEPTEVTPGVHAVSNAALDTPWPKALRAAA
                     GLRRALAAGGAIDPEALFEVLADRQIAADDELPSTGVPLEVERLLSAPFIVAPGYGTR
                     ASTLLLAGRDGRAELLERRFDASFRESGTSRFELEFPGWNGVFETRFPPS"
     gene            8070..>8864
                     /locus_tag="P5164_09720"
     CDS             8070..>8864
                     /locus_tag="P5164_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44211.1"
                     /translation="MSLLQRGLMFVLAAGTLAIPSSVPPDTRAGGRAAAPVEPQTVSV
                     YTPADKEFYLTEDQMDFIRPGFKVVFGAVTDMAPGKKPSVEVTLLDQFDQPLDRLGAK
                     TPGVISLRFIPATWDPATGYYDDLITSGGYPSRDNTGKWEDLGNGKYKYTFNATMPSF
                     DVNKPMTLAVTGNRNMVDYLGKSYYVNTFKDFVPATGAAATTWAVTTTAKCNACHDPI
                     VGHGSNYREAKTCALCHNKNDMVGDSQQYDMGIWHRIHSSNLEDVGE"
BASE COUNT         1313 a         3199 c         3136 g         1216 t
ORIGIN      
        1 tgggaccaga acgccggctg ctccggctgg ctctacggcc tccacatcgc cgacggcctg
       61 atccaggcgg ggaaggccag gaacgtcctc ctcgtcggcg ccgagttcct gacgcgctac
      121 gtcgactact ccgaccgggc gacgtgcgtc ctcttcggag acggggccgg ggcgacgatc
      181 ctccgcgcca cgaagtccga ccggggcgtc ctcgcctcga cgatccggag cgacggcgaa
      241 ggggcctgct tcatccagat gcccgggggg ggctcctcgt tcccgccgac gcggccggag
      301 acgctcgagc agcgcctccc gttcatcaag atgatgggcg gggagacgtt caagatcgcc
      361 gtccgctcga tggtcgaggt ctgcaaggac gtcctcaacg agagcggctt cgagccgggg
      421 gaggtctcct ggctcgtccc ccaccaggcg aacgaccgga tcatccaggc cgtcggcgag
      481 aggctcggga tcccgcggga gcgctgcatc gtgaacatcg agcgctacgg gaacacgagc
      541 gcggcgtcga tcccgatcgc cctcgacgag gcggcccggg acggccgcgt caagcgcggc
      601 gacctgatcc tcttcaccgc tttcggcgcc ggcctcacgt ggggcgcggc cctcgtgagg
      661 tggtagcgat ggcgtccctc tctttcctct tttccgggca gggctcccag tacgccggca
      721 tggggcgcga cctcgtcgcc gcccacccgg aggcgcgcgc cgtctacgag cgggccgacg
      781 cggccctcgc gccgttcggc ctcgcgatct cgcgcgtctc cttcgagggg accgacgagg
      841 agctgaagcg gacggcggtg acgcagccgg cgatcctcgt ccactccgtc gccgtcttcg
      901 aggtcctgaa ggctcggggc ctttcgccga cgatgctcgc gggccactcc ctcggggagt
      961 ggtcggcgct cgtggcggcg ggggcgctct ccctcgagga ggcggtcgtc ctcgtccacc
     1021 ggcgcggcac gttcatgcag gaggcggtcc cggcgggaga gggggcgatg ggggccgtga
     1081 tcggcctgga cggcgctctc gtcgccgagg cctgcgccga ggtcgaggcg gcgacggggg
     1141 aggcggtctc ggcggcgaac ttcaactcgc ccgagcagac ggtcatcgcc ggcacgacga
     1201 acgccgtcgc gaaggccggg gagctcctcc gccagaaggg ggcgaagcgg gtcctcccgc
     1261 tgccggtttc cgccccgttc cactgcgcgc tgatgaagcc ggccgaggag aagctcgggc
     1321 cgttcctcga agcggcgccg ttctccgacc tcgccgtgcc ggtcgtcacg aacgtcgacg
     1381 tcctcgcgaa cctctccggc gccgaggcgc gcgaggcgct ccgccggcag gtctgctcgc
     1441 cggtccgctg ggtcgagacc gtgcagcggc tctcgcgcga cacggagggg gcgatcgagg
     1501 tcggccccgg gacggtcctc gcgggccttg cgaagcggat cgcgaaagac tggcccgtcc
     1561 ggacgacgtc ggacgcggag gggatcggga agctcctcgc cggatgacca cgaagacgac
     1621 cacgaacgcc acccgcgtcc tggacgcgcg gaaggccgtc tacgcgctcc ggacgttcgc
     1681 cgcggcggac ttcaccgccg aggaggccgc ggagaagctc ggcgtccctc ccggcgaggt
     1741 ctggaagacg ctcgtggcga agggggaccg gaccggcctc ctggccgcgg tgatcccgac
     1801 cgggacgcgt ctctcgctga agaagctcgc tcgcgcctcg ggcaaccgga cggtcgcgat
     1861 ggtctcgccc gaggagatgg agaaggcgac gggctacgtg aaggggggct gctcgccgtt
     1921 cgggatgcgg aaggcgatgc ctctctacgt ccacgaggcg gcggcggcgc tgccgcgcct
     1981 cttcgtctcg gcgggccagc ggggcgtcca gctcgagatg gcgggcgggg cgctcctcgc
     2041 ggcctccggc gccgccgcca ccgacctcgt cgaggaatag ggggaaagac atggcgcacg
     2101 tgatcacgct cgaggggaag gtcgcgctcg tcaccggggc gtcgcagggg atcggcgagg
     2161 cggtcgcccg ccggctcgcc gccgccgggg cgacggtcct cgccgccgcc cgcagcgagg
     2221 agaaggccgc cgcggtcgcc gcgtcgatcg cggaggcggg aggggccgcc gagagcatcc
     2281 gcctcgacgt ctccgatccg gcctccgtca ccgcagcctt caaggggatc gtcgagcggc
     2341 acgggaagct cgacgtcctc gtcaacaacg ccgggatcac cgacgacggc ctcctcctgc
     2401 ggatgtcgaa ggagagctgg gaccgggtga tcgccaccga cctgaccggc gtcttcctcg
     2461 tctcgcagga ggcggtgaag ctgatgatga agaagcgggt cgcgggacgg atcgtcaccg
     2521 tcggctccgt cgtcggcctc atggggaacc cgggccaggc gaattacgcc gccgcgaagg
     2581 cgggcgtcgt cggcttcacg atgtcgctcg cccgcgagat cgggtcgcgg gggatcaccg
     2641 tgaacgccgt cgcgccgggc tacgtcgaga ccgcgatgac gggcgcgctt tccgacgatc
     2701 agcggaagtc gctcgcggag aagatcgtcc tcggcaggct cggaacgggg gaggacgtcg
     2761 ccggcgccgt cctctacctc gcctccgacc tcgcctcgta cgtcaccgga acggtcctga
     2821 acgtgagcgg cgggctcctc atcccctgac gggagaggag cttggccgcg cttgaattcg
     2881 gcgggaaagc cggtaagaat ccagccgttt ccgccacaag atggaggagt tgaatgtcga
     2941 actcgattga ggacaaggtc aagagcatca tcgtcgagca gctcggggtc caggccgagg
     3001 aggtcaagcc cgaggcgagc ttcgtcgacg acctcggcgc cgactcgctc gacaccgtgg
     3061 agctcgtcat ggccttcgag gaggctttcg gcatcgagat cccggacgag gacgccgaga
     3121 agatccagaa ggtcaaggac gccgtcacct acatcgagtc ccacgccaag cccgcctgag
     3181 ccccggtgac ggcccggatg gaccacctcg acgcccgggg acgccgccgg gtcgtcgtca
     3241 cggggatcgg cctcgtctcg cccgtcggcc tgacgcgcga ggagaactgg ggcgccctcc
     3301 tcgcggggcg ttccggcatc gggcccatca cccgcttcga cgcgtcggac tacgcctgcc
     3361 ggatcgcggg ggaggtgaag ggcttcgacc ccgccctctt cctcgaccgg aaggagatca
     3421 agaagttcga cacgttcgtc cactacgcgg tcgccgccgc gaaggaggcg ctcgcggagt
     3481 cgggcctcgg aatcacggag gagaacgccg aggccgtcgg cgtctgcgtc ggctcgggga
     3541 tcggcggcct ccccctcatc gaggagaccc acaaggtcct cctcgagaag gggccgcgcc
     3601 ggatcagccc gttcttcatc ccgggcctga tcgcgaacat gtcctcgggg ctgatctcga
     3661 tcctgacggg cgcgaagggg ccgaacacgg cgacggtcac cgcctgcgcc acctccgccc
     3721 acgccatcgg cgacgccgtg aacatcatcc gccgcggcga cgcggacgcg atgatctcgg
     3781 gcgggaccga agcggtggtc tcgccgctcg ccgtcggcgg cttcgccgcg atgcgcgcgc
     3841 tctcgacgcg gaacgacgac ccggccgcgg cctcccgccc gtgggaccgg gaccgcgacg
     3901 gcttcgtcct cggcgagggg gccggcatcc tcgtcctcga agagatgggg aacgcgatcc
     3961 ggcgcggagc cgcgatctac gccgaggtgg ccgggtacgg gatgtcgggc gacgcccacc
     4021 acatctccgc cccctccgac gacggcgacg gtccgttccg cgtcatgcgc aacgcgctca
     4081 aggacgccgc gatggagccg ggcgaggtcg ggtacgtcaa cgcccacggc acctcgacac
     4141 ctcacggaga ccggatcgag acgatcgcgc tgaagcggct cttcggggat ggggcgcgga
     4201 acgtgccggt ctcctcgacg aagtcgatga cggggcacct cctcggggcc gccggcggct
     4261 acgaggcggg cgtcctcgcg ctggcgatcc ggcgcggcgt cctgccgccg acggtcaact
     4321 acacgactcc cgaccccgaa tgcgacctcg actacacgcc gaacgtcgcc cgggagaccc
     4381 gggtgcgcgc gggcctctcg aactccttcg gcttcggggg gacgaacgcc tgcctggtga
     4441 tgagggccgt ctgacggccc gccgcgacgg cgccggcgac ccgggccgat catcgtcggt
     4501 cgatcccgta cgggattccc tctatgctcg gagggccgga cgggcccgat gaaagccgac
     4561 ctgattcgcc ggaagttctt cgacggcgtc ccccgctacg agtggatcga gacgctcggg
     4621 cagggaggcg tcgggatcgt ctacaaggcg aaggacctcg agctcgacga ggtcgtggcg
     4681 atcaaggtgc tctcgccgga catcgcgaag gaggacgagg tcgtcctcgc ccgcttcaag
     4741 cgggagatca acctgaaccg gaagatcaag cacccgaacg tcgcccgcat gtacgacttc
     4801 gggatcagcg gcgagtaccc ctacatcacg atggaggtcg tcccggggaa ggacctctgg
     4861 acgatgatcc gtgacgaggg ccggctcccg ccgccgaagg ccgtgccgat cctccgccag
     4921 atcgcgcggg gctgctccgc gatccacgag ctgggcatcg tccaccgcga cctgaagagc
     4981 cagaacgtcg tcgtcgacga gcagggcgcc gtcgccatcg tcgacttcgg cctcgcgcgc
     5041 tggaacctcg gcggcggctt cactctcgac tcgatcatcc tcgggacgcc gcagtacatg
     5101 tcgccggagc aggcatccgg caagacggtc gacgcgcgga ccgacatcta ctccatcggc
     5161 atcatcgcgt tcgaggccct caccggtcag cttccgttca cgggcgacag cccgatcgcg
     5221 gtcgcgatga tgcagatcaa cgaccccgtc ccggacctcc ttccgaagtt cgcggacgtc
     5281 tctccggacc tgcgcgaggt cgtcctgaag gcgcttcgga aggaccccgc gaagaggtac
     5341 gcgacggcgg tcgacctcga agccgacctc gcgagcgccg agcgcctccc gacgccccgc
     5401 gagggggcgc tcgcacgccc cgtcggaggg gacccgctca tggcgcagct ggagtcggtc
     5461 ctgggcgcgg tgatcctccc ggcgcctccg gagaagcccc gtcgtccctc gccctccgac
     5521 gcctcggccc cggtgacgcg cccctcggtc gttccccgtc ccgcggcggc gaagccggct
     5581 ccgactccgg cgaagccggc ccctccgatg gcgccggccc ggccgccgac cgtcctgatc
     5641 gtctcctccg cgccggccga gcgggccgcg tggaaagccg ggctcggcat cgggccctgc
     5701 cgcgtcctcg aggccggcag cggtccggag gcgctcgagg cgctcgttcg ggagccggcc
     5761 gacctcgtcc tgatggccgt cgcgctcccc ggcatggacg gcttcgacgt cacgcgcgtc
     5821 ctcaagtcgc agccgaacct cgcggcgctg ccggtcctcc tcgcctccga ccggctcgag
     5881 cggagccatt acgcgttcgg catccagtcg ggcgcgaacg agctcctcgt caagccgatc
     5941 gagccggtcc gcctgcgcga ggccgtgcgg gggctcctcc gccatcgcgg cttcccgctc
     6001 cccgacggcc ccgcggacgc ggagcccggc gccggaaacg gctgactccg gccgtttccg
     6061 ctttacaacg gcggggcaga gtcccgatca tcggccgatg ccgagcgaat cggagccgga
     6121 cgaccccgtc ggcgaagcgg aagcttccgc ggagcgggtc cgcgtggacg agcggcgcgc
     6181 cggatactgg gacccgaccg cgtcgggacg tcaccagatc agccggccga tcgccatcga
     6241 cgggctccgg gccgagaggg cccggatcga gctcgctcct cgcgtcgtcc ggcgcggcgc
     6301 gacgtactac gacgactcgt accacgtcgt cgacgccgcg gcgcggcagc tcgcgtcgat
     6361 cttcaccctg gagcagctcg tcatggcgac ggtcgccctc cgcgtccaca tgcgccgcgg
     6421 gcgggagcac atcggccgga agaaggagct tcaggagcgt tggaccgtcc tcgcccaccg
     6481 gctcatgaag ctgcgcgccg aggagcggca cctcgccctg cgggagcagc ggatcaccga
     6541 ggagcacgcc agcctcgcgg gcgccgactc ggagcggccg ggccgggccg agctcctcgc
     6601 cctcgaggag gccgaggtcg cccaggcgcg ccagtcctgg cgggacgaga gccggcgggc
     6661 ctgggaggcg tggacccgcc tcgagtccga ggagggcgcc ctcctggcgg gagcgcccgg
     6721 cctcgagcag acggaagacc tgaagcagcc gtacatcctc ctcctcgtcg ccgcgcgacg
     6781 ggcggcgccg cggagcgtcg cggcggcgat gcacacgctc gtcgacgccc tccagcgccc
     6841 cctgtcgcgg accgatcaga tgctcctcga gatcgcgttc cgccgctgca tccgagcggg
     6901 ctcgcccgag gacgagcggg acctcgagcg ctgggagcac gccctcctgg ccgacgccga
     6961 gcgggagttc gcctcggagt tcccgtgggc gcggccgtac ctcgggaacg gctgacgggc
     7021 cggcgagagg aaagacggcc tctccgcgag gccgccggcc cgaaggacgc tcgatgtgcc
     7081 tgatcgtcct cgctctccac gcgcacgctc gccatccgct cgtcgtcgcg gcgaaccggg
     7141 acgaggacct cgaccgaccc accgcgccgg cggccttctg gccgggcgag gccgggctcc
     7201 tcgcgggccg ggacctccgg tccggcggga cctggatggg tgtcacgcgc acgggccgct
     7261 tctccgcgct cacgaacgtt cgcgacccgc gcgcgcacga tcccgtcgcc ccgtcgcgag
     7321 gcgggctcgt cgtccgattc ctcgcgggcg gggccgagcc cctcgcgcat gcccgggcgg
     7381 tggccgccga gcgcgcccgg gcgaacggct tcaacctcct cgccggggcc ggcgggcggc
     7441 tcgcctgggt ctcgaacgcc gggggcgagc cgaccgaggt gacgccggga gtccacgccg
     7501 tctcgaacgc ggccctcgac acgccctggc ccaaggcgct ccgggccgcc gccggcctga
     7561 ggcgcgccct cgcggccggg ggcgcgatcg acccggaagc gctcttcgag gtcctggcgg
     7621 accgtcagat cgccgcggac gacgagcttc cctcgaccgg ggtcccgctc gaggtcgaac
     7681 gactcctctc ggcgcccttc atcgtcgcgc ccggctacgg gacgcgcgcc tcgacgctcc
     7741 tcctcgcggg gcgcgacggc cgggccgagc tcctcgagcg gcggttcgac gcgtcgtttc
     7801 gcgaatcggg gacgagccgc ttcgagctcg agttcccggg ctggaacggc gtattcgaga
     7861 cgcggtttcc gccgtcctga gatcgacctt caggaccgct gaatctggcc cttcagaggg
     7921 ctgatgcggg tgcatcattt tgtcttcgcg cgcgtctttg acctcctcgc ggatgccgaa
     7981 agcacctacg attcgcgcgg attcgttgga tccgaatatg tgcgtcgacc ccagtattcc
     8041 ccgcgacaag gcttgaagga ggagatccca tgtcacttct gcagcgcgga ctgatgttcg
     8101 tcctggcggc ggggacgctc gcgattccgt cgtccgtccc gcccgacacc agggccggcg
     8161 gacgggccgc ggctcccgtg gaaccgcaga ccgtgagcgt ctacaccccg gcggacaagg
     8221 agttctacct gaccgaggat cagatggact tcatccgccc cggcttcaag gtcgtcttcg
     8281 gagccgtcac cgacatggcg cccggcaaga agccgagcgt cgaggtcacg ctcctcgacc
     8341 agttcgacca gccgctcgac cgcctcggcg cgaagacccc gggcgtgatc tccctccggt
     8401 tcatcccggc gacgtgggac cccgcgaccg gctactacga cgacctgatc acgtcgggcg
     8461 gctatccttc ccgcgacaac accggcaagt gggaagacct cgggaacggc aagtacaagt
     8521 acacgttcaa cgccacgatg ccgagcttcg acgtgaacaa gccgatgacg ctcgccgtca
     8581 ccggcaaccg gaacatggtc gactacctcg ggaagtccta ctacgtgaac acgttcaagg
     8641 acttcgtccc ggcgacgggc gcggcggcga cgacctgggc cgtcacgacg accgcgaagt
     8701 gcaacgcctg ccacgatccg atcgtgggcc acggcagcaa ctaccgcgag gcgaagacct
     8761 gcgccctctg ccacaacaag aacgacatgg tgggcgacag ccagcagtac gacatgggga
     8821 tctggcaccg gatccactcc tcgaacctcg aggacgtcgg cgag
//
LOCUS       DAOTEZ010000158         8863 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5600_length_8863_cov_6.966281, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000158 DAOTEZ010000000
VERSION     DAOTEZ010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8863
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5600_length_8863_cov_6.966281"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /gene="carB"
                     /locus_tag="P5164_09725"
     CDS             complement(<1..438)
                     /gene="carB"
                     /locus_tag="P5164_09725"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358665.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HRY44212.1"
                     /translation="MPKRADLKSILVLGSGPIVIGQACEFDYSGTQACRALRSEGYRV
                     VLVNSNPATIMTDPEFADATYVEPLDVATVGRVIAKERPDALLPTVGGQTALNLAVAL
                     EKEGVLERYGVELLGASARSVELGEDRLLFKEAMLRAGVDVLRS"
     gene            622..2448
                     /locus_tag="P5164_09730"
     CDS             622..2448
                     /locus_tag="P5164_09730"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRY44213.1"
                     /translation="MKLPEGLSFDHVPELELLWAELSEMRLVLGSLPFLVCAVDGEGL
                     FSFWNAECERVTGYTAGDVVRSPGAADLLFPDPLQKARMCELWREAGRGFRNVEVELA
                     TKAGAVRTVSWSTLAAAGPTEDRDVWAVGVDRTDARRNEQVLTLVAEVNARLLAREPL
                     QKIHAYVCRTVAEVFRFGLVCIGQRQPDGTVRMSGLAGPHAPEAVAHEIRWDRPSPHG
                     GITGAAIRTGLVQRAEARNGDAFGGTWRNRSAGHRIGASIALPLVDGGTAVGALTVHA
                     PNANAFDTRTVDLLLRLTQQISLSFDHAAGLTEIRLQSLALDAVHHSVVITAPDGTIE
                     WVNPAFTEMTGFSREEAVGKTPRIQSSGRHERAFFEELWRTILSGRTWEGETTNCRKD
                     GSVYVEDQTITPVRDENGEVAHFVSVKRDITQRKRYEEAIVHLSRHDPLTDLPNRRVV
                     EEDLVRLLARAERGLPSALLLVDLDNFKEVNDRSGHGSGDSLLRAFARLFLDAVRPGD
                     LVGRIGGDEFVILLEGAGAEEARAVADRVRDRAAALATPEGYDGPAVGASIGVALVAG
                     HAQPADVLATADAALYDAKSEGKGRVSVRTRPAPPRSGSWKA"
     gene            complement(2461..3213)
                     /locus_tag="P5164_09735"
     CDS             complement(2461..3213)
                     /locus_tag="P5164_09735"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HRY44214.1"
                     /translation="MSALLWPDGGGALFDGLPSGLSAGFTTLDLVPDELPADDGARRF
                     ARMLGVPEAPVARLKQVHGAAVVEARGIAPGEVAVAGAADAVVTTERGLVVAVVTADC
                     VPIVLADLEAGVLAAAHAGWRGTAARVAEAALDALESRGGRAERSWALFGPSISRGRY
                     EVGPEVLAALGDPVPDGAVVAGRGDRSWLDVAAFNEAALLRRGFHPERVVRPELCTFG
                     PPIRFCSYRRDGERAGRIFTGAVLLPGGGAAR"
     gene            complement(3210..3692)
                     /locus_tag="P5164_09740"
     CDS             complement(3210..3692)
                     /locus_tag="P5164_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HRY44215.1"
                     /translation="MPDAALWALALLLVLAGIAGAVLPAIPGAPLVFGGLLLAAWIDG
                     FTKVGWFPLVLIGLLTALTFVVDFAATALGARRVGASPLALVGAAVGTVAGLFFGFVG
                     VLVGPFAGAALGEYAARRDLRQAGRAGLGTWLGIVLGSAAKIALLLAMVGVFAVAWLV
                     "
     gene            3782..4219
                     /locus_tag="P5164_09745"
     CDS             3782..4219
                     /locus_tag="P5164_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRY44216.1"
                     /translation="MTTARDLLARKKSAAVITVAPDATVKDAAHLMHEHRIGALVVAE
                     GSTIRGIFTERDVLWRVVSEGRDAATTPVRDVMTPDVIVVRPDREIDDIEGILMQHRI
                     RHLPVAGEEGLLGMLSIGDLNAFRADADHQKVEYLTEYIYGRS"
     gene            complement(4376..5230)
                     /locus_tag="P5164_09750"
     CDS             complement(4376..5230)
                     /locus_tag="P5164_09750"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRY44217.1"
                     /translation="MTLPGIGVTLGRYRLEERIGAGGMAEVWRALDLGLERFVALKVI
                     SPDAVQSDPSFVPRFLREAKLAARLEHPHIIPIWDFGPSDDLLFLVMPLLPGGSLRDR
                     AREGVPGLVAMDWLEALAAALDYAHGEGIVHRDVKPANVLFDRNGRLFLADFGLARES
                     GSAGLTVAGTVLGTPVYMSPEQVRGEPAGPRSDQYALGVLAYYLLAGTPPFEANSVFV
                     VMEKTLREEPALPSDINPGLHRSADYVLLKVLEKRPENRFESCREFVEALQRASRRGT
                     DPGVKTRA"
     gene            5397..6248
                     /locus_tag="P5164_09755"
     CDS             5397..6248
                     /locus_tag="P5164_09755"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HRY44218.1"
                     /translation="MEGTGGRYGYEPGCFRPPAGREGDAKLLDTLRALGLELEVVEER
                     AGDAPGALALNGLPAYARVARLLSLAARLGRGTLPRRAPTVVSPCTACYRNLSRARSA
                     LATHPDLREQVDACLADEGFRCEPGAVRVRHLLDVLWEDVGPGAVRARVSRPLTGLRV
                     AAYYGCLDARDGGARDARTAGFETRFEQLLAALGADVVDFPLRSHCCGGRASEASAEV
                     AGSLVGRILRSAAERGVDVIAVACPRCRHNLRSRRATPPAEAPAQPEVPARYFTELFA
                     EAFGLAP"
     gene            complement(6203..7879)
                     /locus_tag="P5164_09760"
     CDS             complement(6203..7879)
                     /locus_tag="P5164_09760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY44219.1"
                     /translation="MDGPVDLDRERRREGETSPAAAFLDLHAILESIPEPVGLVDHAG
                     RILAFNGPARRVVFAARSQAVALGDDLFSFISPHNLAGVRVSLERAFAGEAHTHRTEA
                     AGRVFETVYSPVRGPCGEVAAVSIRVKDVTERERALAEIASLNATLEERVARRSEELE
                     SVNAELGKAARAKDAFLASMSHELRTPLTGILGAAELLRVGAHGPLNEAQLRTLRTLE
                     EAGRHLLSILADLLDLAKIGAERLSLRADACVLADVCESALSMVRNEAQRKGIALVLR
                     APDAEVRFGADARRVRQVLVNLLSNAVKFTPAGGTVELAAQGDADAGLVRLEVKDSGP
                     GIADEDLPKLFQPFTQLDTRLSREHAGTGLGLALVRGLTELHGGDVEVESATGRGSVF
                     RVTLPWRPPSARGSGAHRVLAAEPPPAPGGGSAARVLLVEDDDANRTILGEFLRSRGF
                     VVEEAASGTEALERVAACRPDLVVLDIQLPGMDGLEVLARLRAAAAAPPPVLALTALA
                     MSGDRERILAAGADAYLAKPAPLAVLEQEVRRLAAAQGASPNASANSSVK"
     gene            7928..8464
                     /locus_tag="P5164_09765"
     CDS             7928..8464
                     /locus_tag="P5164_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44220.1"
                     /translation="MLTVYLAALAFGLVLIGASVFHGDGDSHDTGDDHGGGDADHGAF
                     LSNLFSLRFWTYALGAFGMAGTALSLLGVGAPVHVPVSVVLGFVVGTGVATLFRSLGR
                     GAATSPASTEAFLGTEGEVVLPLLPDGLGKIRLHVADQDVEVPARTGGEPIQIRERVV
                     VVRFRDGIAEVEPAPWKE"
     gene            8490..>8863
                     /locus_tag="P5164_09770"
     CDS             8490..>8863
                     /locus_tag="P5164_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013321879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRY44221.1"
                     /translation="MLDSLAGIGAGVVAGAVSLGILSLLAKSILVICPPNQVVIFSGR
                     KRKLADGSQVGYRVVLGGRSVRIPILERVDRMSLLSIPIDLKVANAYSKGGIPLRVHA
                     IANVKVSSDPRVVGNAIERFLGR"
BASE COUNT         1205 a         3388 c         3147 g         1123 t
ORIGIN      
        1 cgagcgaagg acgtcgacgc cggcccggag catcgcctcc ttgaagagga gacggtcctc
       61 ccccagctcg accgacctcg ccgaagcgcc gaggagctcg acgccgtacc tctcgaggac
      121 cccctccttc tcgagggcga ccgcgaggtt cagggccgtc tgcccgccga ccgtcgggag
      181 gagggcgtcg ggacgctcct tcgcgatcac gcgccccacc gtcgcgacgt cgagcggctc
      241 gacgtacgtc gcgtcggcga actcggggtc ggtcatgatc gtggcggggt tcgagttgac
      301 gaggacgacc cggtacccct cgctccggag ggcccggcag gcctgggtcc ccgagtagtc
      361 gaactcgcac gcctgcccga tgacgatggg accggacccg aggaccagga tcgacttcag
      421 gtcggctctc ttcggcacgg ggcggagtct aagggaacgc cgcgcggcgg gccgggctcg
      481 catcggcgaa cgctccgccg cggccgggac ggctcccgga gacgctcccg gaaggacttg
      541 acggcctggc ccccgattct ggatgatcgg acgtcgaccc cagccccgga cgtccgttct
      601 cccgagcctc cccggaggtc catgaagctc cccgagggcc tcagcttcga ccacgtcccc
      661 gagctcgagc tgctctgggc ggagctgtcg gagatgcgcc tcgtcctcgg ctcgcttccc
      721 ttcctcgtct gcgccgtgga cggagagggc ctgttctcgt tctggaacgc cgagtgcgag
      781 cgggtcacgg gatacacagc cggcgacgtc gtccggagcc ccggcgcggc ggacctgctc
      841 ttccccgacc cgcttcagaa ggcccggatg tgcgagctct ggagggaagc cggccgcggt
      901 ttccggaacg tcgaggtcga gctggcgacg aaggccgggg cggtccggac cgtctcctgg
      961 tcgacgctcg ccgccgccgg tccgaccgag gaccgggacg tctgggccgt cggcgtcgac
     1021 cggaccgacg cacggcggaa cgagcaggtc ctcacgctcg tcgccgaggt caacgcccgc
     1081 ctcctcgccc gcgagccgct ccagaagatc cacgcctacg tctgccgcac ggtcgccgag
     1141 gtcttccgct tcggcctcgt ctgcatcggt cagcgccagc ccgacggcac cgtccggatg
     1201 agcggcctcg ccggtccgca cgccccggag gccgtcgccc acgagatccg ctgggaccgc
     1261 ccctctcccc acggggggat cacgggcgcg gcgatccgga ccggcctcgt ccagcgcgcc
     1321 gaggcgcgga acggagacgc cttcggcggc acctggcgaa accgctcggc cggccaccgg
     1381 atcggcgcct ccatcgcgct ccccctcgtc gacgggggaa cggccgtcgg cgccctcacg
     1441 gtccacgccc cgaacgcgaa cgcgttcgac acgcggaccg tcgaccttct cctgcggctc
     1501 acccagcaga tctcgctctc gttcgaccac gcggccggtc tgaccgagat ccggctgcag
     1561 tcgctcgccc tcgacgccgt ccaccactcc gtcgtcatca ccgcgccgga cgggacgatc
     1621 gagtgggtca accccgcgtt caccgagatg accggcttct cgcgcgagga ggccgtcggg
     1681 aagacgcccc ggatccagtc ctcggggcgg cacgagaggg cctttttcga ggagctctgg
     1741 aggacgatcc tctcggggcg cacctgggag ggagagacga cgaactgccg gaaggacggc
     1801 tcggtctacg tcgaggacca gacgatcacc cccgtccggg acgagaacgg cgaggtcgcc
     1861 cacttcgtct ccgtcaagcg ggacatcacc cagcgcaagc gctacgagga ggcgatcgtc
     1921 cacctctcgc ggcacgaccc cctcacggat ctcccgaacc gccgggtcgt cgaagaggac
     1981 ctcgtccggc ttctcgcgcg cgccgagcgc ggcctcccct ccgcgctcct cctggtcgac
     2041 ctcgacaact tcaaggaggt caacgaccgt tcgggccacg gctcgggcga ttcactcctc
     2101 cgcgcgttcg cccggctctt cctcgacgcc gttcggccgg gcgacctcgt cggacggatc
     2161 ggcggagacg agttcgtgat cctcctcgag ggggcgggag cggaggaggc acgcgccgtc
     2221 gctgatcggg tccgcgaccg cgccgcggcc ctcgcgaccc ccgaggggta cgacgggccg
     2281 gccgtcggcg cgagcatcgg cgtcgccctc gtggcgggcc acgctcagcc cgccgacgtc
     2341 ctcgcgacgg ccgatgccgc cctctacgac gcgaagtccg aggggaaggg gcgcgtctcc
     2401 gtccggaccc ggcccgcccc cccgcgcagc ggcagctgga aggcctgacg gccccgccgt
     2461 tcagcgcgcc gcgccgccgc ccgggaggag caccgcgccg gtgaagatac gtccggcccg
     2521 ctccccgtcg cggcggtagg agcagaagcg gatgggcggc ccgaaggtgc agagctccgg
     2581 gcggacgacc cgctccgggt ggaacccccg ccgcaggagg gccgcctcgt tgaacgccgc
     2641 cacgtcgagc cagctccggt cgccgcgccc cgcgacgacc gcaccgtcgg ggaccgggtc
     2701 gccgagggcg gcgaggacct ccggacccac ctcgtagcgg ccccgcgaga tcgacgggcc
     2761 gaagagcgcc caggagcgct ccgcccggcc tccgcgggac tccagcgcgt cgagcgccgc
     2821 ctcggcgacg cgtgcggccg tccccctcca cccggcgtgc gccgcggcga ggacgcccgc
     2881 ctcgaggtcg gcgaggacga tcgggacgca gtcggccgtc acgaccgcga cgacgaggcc
     2941 gcgctccgtc gtcacgaccg cgtcggccgc gccggcgacg gcgacctcgc cgggggcgat
     3001 cccccgcgcc tcgacgaccg ccgcgccgtg gacctgcttc aggcgggcga cgggcgcttc
     3061 gggaacgccg agcatccggg cgaagcgccg cgcgccgtcg tcggcgggga gttcgtccgg
     3121 cacgaggtcg agcgtcgtga agccggcgga gagcccggac ggcagcccgt cgaacagcgc
     3181 cccgccgccg tccggccaga ggagcgcgct cacacgagcc acgccacggc gaagacgccg
     3241 accatcgcga ggagcagggc gatcttcgcg gcgctcccga ggacgatgcc gagccacgtc
     3301 ccgaggccgg cccgcccggc ctgccgcagg tcgcgccgcg cggcgtactc cccgagcgcc
     3361 gcgccggcga acgggcccac gaggacgccg acgaagccga agaagagccc cgccaccgtc
     3421 ccgacggcgg cgccgacgag agccagcggg ctcgcgccga ctcgcctggc cccgagcgcc
     3481 gtggcggcga agtcgacgac gaacgtgagc gccgtcagga ggccgatgag gacgagcgga
     3541 aaccagccga ccttcgtgaa gccgtcgatc cacgccgcga ggagcagccc gccgaagacg
     3601 agcggcgccc ccgggatcgc cggcaggacc gcccccgcga tcccggcgag gacgaggagg
     3661 agggccaggg cccagagggc ggcgtcgggc acggcacgca ggataccaag cggtcgcgct
     3721 gcgccgcggc ggctgcggcg cagcgttgac gcatccgcgc cgcaccgtat agaagtcgtc
     3781 catgacgacc gcacgcgacc tcctcgcccg gaagaagtct gccgcggtga tcaccgtggc
     3841 gcccgacgcc accgtcaagg acgccgccca cctcatgcac gagcaccgga tcggtgcgct
     3901 cgtcgtcgcg gaaggctcga cgatccgcgg gatcttcacg gagcgcgacg tcctctggcg
     3961 cgtcgtctcc gaggggcgcg acgcggccac gactcccgtc cgcgacgtca tgacgcccga
     4021 cgtgatcgtc gtccggccgg accgcgagat cgacgacatc gaggggatcc tgatgcagca
     4081 ccggatccgc cacctccccg tggcgggcga ggaggggctc ctcgggatgc tctcgatcgg
     4141 cgacctgaac gccttccgcg ccgacgccga ccaccagaag gtcgagtacc tcaccgagta
     4201 catctacggc cggagctgaa gtggatcggg gaggtgcgcc acgcacctcc tcgcggcgcc
     4261 ttcggcgcct cccctccttg ggagacgggg atcggggagg tgcgccacgc acctcctcgc
     4321 agcgcgttcg gcgcctcccc tcctccgggc cggaaccgga tcggggaggc gggcgctacg
     4381 cgcgcgtctt cacgccgggg tcggttccgc ggcgcgaggc gcgctggagc gcctcgacga
     4441 actcgcggca cgactcgaag cggttttccg ggcgcttctc gaggaccttc agcaggacat
     4501 agtcggccga gcggtggagc cccgggttga tgtccgacgg cagcgccggc tcctcccgga
     4561 gcgtcttctc catgacgacg aagaccgagt tcgcctcgaa cggcggcgtc cccgcgagga
     4621 ggtagtacgc gaggacgccg agggcgtact ggtcggagcg cggccccgcc ggctcgcccc
     4681 tcacctgctc gggcgacata tagacgggcg tcccgaggac cgtcccggcc accgtcaggc
     4741 cggcgcttcc tgactcgcgc gccagcccga agtccgcgag gaagagcctc ccgttccggt
     4801 cgaagaggac gttcgcgggc ttcacgtcgc ggtggacgat cccctcgccg tgcgcgtagt
     4861 cgagcgcggc ggcgagcgcc tcgagccagt ccatcgcgac gaggcccggg acgccctccc
     4921 gcgcgcggtc ccggagggag ccccccggca ggagcggcat gacgaggaag aggagatcgt
     4981 cggacggacc gaagtcccag atcgggatga tgtgggggtg ctcgagccgc gccgcgagct
     5041 tcgcctcacg caggaaccgt gggacgaacg acgggtcgga ctggacggcg tccggggaga
     5101 tcaccttcag ggcgacgaag cgctcgagcc cgagatcgag cgcccgccag acctccgcca
     5161 tgccgcccgc cccgatccgt tcctcgagcc gataccggcc gagtgtcacg ccgatgcccg
     5221 gaagcgtcac caacacctcc ccgcccgtgc ggcgcgagag ccccgagcgc acggtctcga
     5281 ttcgtcctgt ccgtggtgcc ggcagtatat tccgcggcct ttcggcgccc gccggggcgg
     5341 aattgatgcg cccggatcat ggtgcgggac gacgcggccg gccaatcatc gtccgcgtgg
     5401 aggggacggg cggacgatac ggctacgagc cgggctgctt ccgccccccg gcggggcgcg
     5461 agggggacgc gaagctcctc gacaccctcc gcgcgctcgg gctggagctc gaggtcgtgg
     5521 aggagcgagc cggcgacgct ccgggagccc tcgcgctgaa cggccttccg gcctacgcgc
     5581 gagtcgcacg actcctctcc ctcgccgctc gcctcggtcg cggcacgctc ccgcggcggg
     5641 cgcccacggt cgtcagcccg tgcaccgcct gctaccggaa cctctcccgc gcccggagcg
     5701 cgctcgcgac tcaccccgac ctccgcgagc aggtcgacgc ctgcctcgcc gacgaagggt
     5761 tccggtgcga gcccggcgcc gtccgggtcc gtcacctcct ggacgtcctc tgggaggacg
     5821 tcgggcccgg ggcggtccgt gcacgcgtct cgcgtccgct cacggggctg cgggtcgcgg
     5881 cctactacgg atgcctcgac gcccgcgacg gcggcgcgcg ggacgcccgg acggccgggt
     5941 tcgagacgcg cttcgagcag ctcctcgcgg cgctcggggc cgacgtcgtc gacttccctc
     6001 tccggtcgca ctgctgcggg gggcgcgcct ccgaagcgag cgcggaggtc gccgggtcgc
     6061 tggtcggccg gatcctccga tccgccgccg agcggggtgt cgacgtgatc gccgtcgcct
     6121 gcccgcgctg ccgccacaat ctccgcagcc gccgcgcgac gcctcccgcg gaggccccgg
     6181 cgcagccgga ggtccccgcg cgctacttca ccgagctgtt cgccgaggcg ttcgggctcg
     6241 ctccctgagc cgccgcgagg cgccgcacct cctgctccag cacggcgagc ggcgccggct
     6301 tggccaggta ggcgtcggcc ccggccgcca ggatcctctc gcggtcgccg gacatcgcca
     6361 gcgccgtcag cgcgaggacg ggaggcggcg ccgcggcggc cgcgcggagc cgcgcgagaa
     6421 cctcgagccc gtccatgccc gggagctgga tgtcgaggac gacgaggtcg ggccggcagg
     6481 ccgccacgcg ctccagagcc tccgttcccg aggccgcctc ctcgacgacg aagccgcgcg
     6541 accggaggaa ctcgccgagg atcgtccggt tcgcgtcgtc gtcctcgacg aggagcaccc
     6601 gggccgcgga gccgccgccc ggcgccgggg gcggctccgc ggcgaggacg cggtgcgcgc
     6661 ccgagccgcg cgcgctcggc ggacgccacg ggagcgtcac gcgaaagacg ctccccctgc
     6721 cggtcgccga ctcgacctcc acgtcaccgc cgtgcagctc cgtgaggccg cggacgaggg
     6781 cgaggccgag gcccgtgccg gcgtgctcgc gcgagaggcg cgtgtcgagc tgggtgaagg
     6841 gctggaagag cttcgggagg tcctcgtccg cgatccccgg gccggagtct ttcacctcga
     6901 gacggacgag gccggcgtcg gcgtcgccct gcgcggcgag ctccaccgtg ccgccggccg
     6961 gcgtgaactt caccgcgttc gacaggaggt tcacgaggac ctgacggacc cgccgggcgt
     7021 cggccccgaa gcggacctcc gcgtcggggg cgcggaggac gagcgcgatc cccttccgct
     7081 gcgcctcgtt cctcaccatc gagagcgccg actcgcagac gtccgcgagg acgcaggcgt
     7141 ccgcgcgcag cgacaggcgc tccgcgccga tcttcgcgag gtcgaggagg tcggcgagga
     7201 tcgacagcag gtgcctcccc gcctcctcga gcgtccggag cgtccggagc tgcgcctcgt
     7261 tcagcggccc gtgcgcgccg acgcgcagga gctcggcggc tccgaggatc cccgtcaggg
     7321 gcgtccggag ctcgtggctc atgctcgcga ggaaggcgtc cttggctcgg gccgccttcc
     7381 cgagctccgc gttcacgctc tcgagctcct ccgaccttcg ggcgacccgc tcctccagcg
     7441 tcgcgttgag ggaggcgatc tcggcgagcg cccgctccct ctccgtgacg tccttcacgc
     7501 ggatcgagac cgccgccacc tcgccgcacg gccctcggac cggggagtag accgtctcga
     7561 aaacgcggcc ggccgcctcg gtccgatgcg tgtgcgcctc gcccgcgaac gcccgctcga
     7621 ggctgacccg aacgcccgcc aggttgtggg gcgagatgaa cgagaagagg tcgtccccga
     7681 gggcgacggc ctgcgaccgc gccgcgaaga cgacccttcg ggccggcccg ttgaacgcga
     7741 ggattcgccc ggcgtggtcc acgaggccga ccggctccgg gatcgactcc aggatcgcgt
     7801 gaaggtcgag gaacgccgcc gcgggagacg tctccccctc ccgccttcgc tcgcgatcca
     7861 ggtccacggg cccgtccacc gtgggcgaag tctagcgccc gcggaggcgg cgtaaactcg
     7921 tcccgaaatg ctgaccgtct acctcgccgc gctggccttc ggcctcgtcc tgatcggggc
     7981 ctccgtcttc cacggggacg gggacagcca cgacaccggg gacgatcacg gcggcggcga
     8041 cgccgaccac ggcgccttcc tctcgaacct cttctctctc cgcttctgga cctacgccct
     8101 cggggcgttc ggcatggcgg ggacggccct ctcgctcctc ggcgtcggcg ccccggtcca
     8161 cgtccccgtc tcggtcgtcc tcggcttcgt cgtcggcacg ggcgtcgcga ccctcttccg
     8221 gtccctcggc cgcggggccg cgacgagccc ggcctcgacc gaggcgttcc tcgggacgga
     8281 gggcgaggtc gtcctcccgc tcctccccga cggcctcggg aagatccgcc tccacgtcgc
     8341 cgaccaggac gtcgaggtcc ccgcccggac gggcggcgag ccgatccaga tccgcgaacg
     8401 cgtcgtcgtc gtccgcttcc gggacgggat cgccgaggtc gagcccgctc cctggaagga
     8461 atagctaccc ccagagccag gaggtcgtcg tgctcgactc tctcgccgga atcggagcag
     8521 gtgtcgtcgc gggtgccgtc tccctcggta tcctctcgct cctcgcgaag tcgatcctcg
     8581 tcatctgccc gccgaaccag gtcgtcatct tctcggggcg caagcggaag ctcgccgacg
     8641 ggagccaggt cggctaccgg gtcgtgctcg gcgggcggtc cgtccggatc ccgatcctcg
     8701 agcgggtcga ccggatgagc ctcctgtcga tcccgatcga cctgaaggtg gcgaacgcct
     8761 actccaaggg cggcatcccg cttcgggtcc acgcgatcgc gaacgtcaag gtctcctccg
     8821 acccgcgcgt cgtcgggaac gccatcgagc gcttcctcgg gcg
//
LOCUS       DAOTEZ010000159         8802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5645_length_8802_cov_5.901566, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000159 DAOTEZ010000000
VERSION     DAOTEZ010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8802
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5645_length_8802_cov_5.901566"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="P5164_09775"
     CDS             <1..433
                     /locus_tag="P5164_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY44222.1"
                     /translation="VTEPLELSVGGVRAVVRPCGPAHTPGDLRVDLPDLGVVVTGDLV
                     FHGYHPNLEHADLDGWRRGLEALLCEPAGTVFVPGHGAPGRREIVEAQLAWFEAAREE
                     AGRARADAGGDGLVERLLVRFPDHRLAAVLPTAARVLALGT"
     gene            complement(445..1305)
                     /locus_tag="P5164_09780"
     CDS             complement(445..1305)
                     /locus_tag="P5164_09780"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRY44223.1"
                     /translation="MNLSRNLACAVLLLVASPAFGAGLADFKGYADPALFTRMPGAFL
                     SGVGAYKESAFDAYDFPVKNGAKVEKVRVEGRKTVYTYSFDRAAGPVPSGLQVKRNYQ
                     AAAKRIGGEVLHDSDGAYMNTTLRIPKDGRETWVEVLTRAATYYVTIVERQEMKQDVV
                     ANADALKGGLARTGHVEVPGIFFDFGKADVKPESEPALREVAKLLQASPGLRVWVVGH
                     TDSVGSAEDNVALASGRAASVVAWLTGKLSVDGKRLVPHGVGPYAPVASNATDEGRAK
                     NRRVELVEIR"
     gene            complement(1492..1902)
                     /locus_tag="P5164_09785"
     CDS             complement(1492..1902)
                     /locus_tag="P5164_09785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HRY44224.1"
                     /translation="MTAAERHRDLVTGNRQMDEEHALQLSLLRELVENLDSGDAPAAL
                     EVFERLEAFTRAHFLAEELLMRLHAYPASEAHAADHAAFVRELQGVRARLADGLDEAA
                     REDADAFARRLLSHIASADHAFADFQRAGGAAAS"
     gene            complement(1925..3118)
                     /locus_tag="P5164_09790"
     CDS             complement(1925..3118)
                     /locus_tag="P5164_09790"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRY44225.1"
                     /translation="MLEPTIDPAKLDEWARLLVTHSIGGVTPDDRVMIKGERIAWPLM
                     ERIEREVIAAGGIPDVCLVAPNNERGKVWSAAMGRGATAEQLARVPDWHRDRYASMTK
                     YIEVLGAESPSSYAGLSREAAAALAKADRPFADLRLSKRWVITLYPSPAYAGIEGLPL
                     EEYVRFVVDASTVDPLPLLEAEKRLEPLFADGKRMEVVTFHPVEKRELTLEMSLAESI
                     PVLSHGLRNVPDGEVFTSPDASSVEGELFLDLPVLNGGVDVSGMHLVFEKGRIVRFSA
                     LEGEAQLASIVGTDEGARRLGEVALGMNPGLTRALKHPLFVEKVGGTLHVAIGASYET
                     CFERDPKAPGARERLEELAARGVLNRSAQHVDVVCDFRPGGCGRRVAIDGRPIVVRDG
                     LWVPG"
     gene            complement(3226..3960)
                     /locus_tag="P5164_09795"
     CDS             complement(3226..3960)
                     /locus_tag="P5164_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44226.1"
                     /translation="MRRAVLSAGFALALLSPLALAHRPARADALADLRAALDRAPAGE
                     TVRVRVAIARTDSEKEKPKGERSGEAVVEHGPSGLSIHLDPAFLPKPGSKAERKAREE
                     KTVRLEPGDALELVDPGTELRQLLDGATVVSDRPEAFEGRTARTIVFRVVPDLDDEAR
                     KAIKRYDDVVTLRLDADGLPFALERTLDLKAKKLLVSFTVSMRESRRFTRVAGRLVTA
                     SAREESHSSGLGQESASTTRWTVTPL"
     gene            complement(3999..7310)
                     /locus_tag="P5164_09800"
     CDS             complement(3999..7310)
                     /locus_tag="P5164_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRY44227.1"
                     /translation="MRLRAAGLLALFALLALVLSPQPASAGDPTKMLTQPALSAERVA
                     FVYGNDLWTARLDGSNVQRITSGPGVKASPAFSPDGALLAFTAELDGNLDVFVVPATG
                     GVPKRLTFHPSRDVVQGFTPDGKAVLFASPREATNNRHDQLYTVPVEGGVETKLPIPY
                     ASRAVFSPDGGTIAYTPNRPAHLQWKRYRGGTVSRIWLYDVATHAIVKVPQPEGRCND
                     LNPTWVGGTLYFTSDRDGELNLYTFDAKSRNVAKVTAHEDFPVLAAAAANGKVVYEQA
                     GLLHLLDAATKKAAPLPITVNADLLETRPRWAKGAQWVRDASLSPSGARVALEFRGEI
                     VTVPAEKGDPRYLTATPGAHERGPAWSPDGKSIAYVSDEGGEYRLVVAAQDGKGTPKR
                     IAVPGSGFYDRLAWSPDATKIALTDNSWTLWWIDVASGKAVKVASERLYAPQKTLKAS
                     WSPDSRWLAYTLAGATYIRTLHVHDTVDGKSFPLTDGLSDVSDPVFDKGGKLIWFLAS
                     TDAGPVRNWFSLVNQDMDVTRAIYVATLKAGTPSPLAKESDEEKGEGKDAAKDGKKDE
                     DGKPADGKGGKDAKKDDAKKEEKKPEPVVIDRDGFSLRIVDVPVKAADISGLSSPNAG
                     EVWFLREADGKTALQKWDTKSRKAETIAADVDDYEVSFDGKKLLTRAKESFEVGPAGK
                     RGEAKPGEGNGKLKLDAVQVRVDPRAEWPQILEEVWRINRDYFYDPGMHGRDWKAIRA
                     KYEPFLPHLSSRADLTRLIQWMCSELAVGHHRTFGGDRLDEATPVPGGLLGADYEVAS
                     GRYRFRKVFGGLNWNPELRAPLSEPGVEVKAGEYLLAVNGKELAPPENLFSRFEATAG
                     KIVEITVGPTPDGKGSRTVNVVPVADEGALRNRDWVEGNLAKVEKATGGRVAYVYVPN
                     TAGLGHTYFKRYFYPQAWKDAIIVDERFNGGGSVADYYIEALRREPIAWWTFRYGDDM
                     KTPSASIQGPKVMLIDETAGSGGDLLPWMFRKFKVGPLVGQRTWGGLVGILGFPVLMD
                     GAQVTAPNLAFWAPEEGFGVENVGVPPDVEVEQTPADVIAGRDPQLEKAIEIVLEQLK
                     KSPPVAPKRPPFPVR"
     gene            7443..8417
                     /locus_tag="P5164_09805"
     CDS             7443..8417
                     /locus_tag="P5164_09805"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRY44228.1"
                     /translation="MVTTHTPESLSSLARGFMESRVFLTAAELDLFTLLADGPLSLEE
                     AAAARGADRRALAILLDALTAMGLLEKADGRFTTPPSLVPLLTSRSGGSLLPLALHSV
                     NLWDRWSRLTETVAGARPVAGDGWTRSFIGAMHAIAAPQADAIVAAVGPGSARRLLDV
                     GGGPATYTLAFLRAAPGLRATLFDLPEVVEIARENATRAGLVDRVDLVAGDLRTDALP
                     GGHDLAFVSAIVHMLGPAGNVALFRKVHDALLPGGRVVLRDHVMSPDRTAPRAGALFA
                     VNMLVGTKDGGTFTLEELSAWLEEAGFVRPRLLRDGERMDALVEAVRP"
     gene            8574..>8802
                     /locus_tag="P5164_09810"
     CDS             8574..>8802
                     /locus_tag="P5164_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44229.1"
                     /translation="MVVTASLFSSVPSGPFQPEHPNRSLEGIASEIRRAYPQGVDVVV
                     FLPAVQNGWSLVANRQGIIAILIGLLLPAVQK"
BASE COUNT         1123 a         3106 c         3241 g         1332 t
ORIGIN      
        1 cgtgacggaa ccgctcgagc tctccgtcgg gggcgtccgg gcggtcgtcc gcccctgcgg
       61 cccggcccac acgccgggcg acctgcgggt cgacctgccc gatctcggcg tcgtcgtcac
      121 cggggacctc gtcttccacg gctaccaccc gaacctcgag cacgccgacc tcgacggctg
      181 gaggcgcggg ctcgaggctc tcctgtgcga gcccgccggg acggtcttcg tcccggggca
      241 cggcgcgccg gggaggcggg agatcgtcga ggcgcagctc gcctggttcg aggcggcgcg
      301 ggaggaagcc gggcgcgccc gggcggacgc cggcggggac gggctcgtcg agcgcctcct
      361 ggtgcgcttc ccggaccatc gcctcgccgc ggtcctcccg acggcggcgc gcgttctcgc
      421 gctcgggacc tgaggggcgg cgctctaccg gatctcgacg agctcgaccc ggcggttctt
      481 cgcccggccc tcgtcggtgg cgttcgaggc gaccggggcg tacgggccga cgccgtgggg
      541 aacgagccgc ttcccgtcga ccgagagctt cccggtgagc caggcgacga ccgaggcggc
      601 gcggccggag gcgagggcga cgttgtcctc ggcggagccg acggagtcgg tgtggccgac
      661 gacccagacc ctgaggccgg ggctcgcctg gaggagcttc gcgacctcgc gcagggccgg
      721 ctccgactcg ggcttgacgt cggccttgcc gaagtcgaag aagatgccgg ggacctcgac
      781 gtggccggtc cgggcgagcc cgcccttgag cgcgtcggcg ttcgcgacga cgtcctgctt
      841 catctcctgc cgctcgacga tcgtcacgta gtaggtggcg gcgcgggtga ggacctccac
      901 ccacgtctcc cgcccgtcct tcggaatgcg gagcgtcgtg ttcatgtagg cgccgtcgga
      961 gtcgtggagg acctcgccgc cgatccgctt cgccgcggcc tggtagttcc gcttcacctg
     1021 gaggccgctc ggcaccggtc ccgcggcgcg gtcgaacgag taggtgtaga cggtcttgcg
     1081 tccctcgacc ctcaccttct cgaccttcgc gccgttcttc acggggaagt cgtaggcgtc
     1141 gaacgccgac tccttgtagg cgccgacgcc cgagaggaag gcgcccggca tccttgtgaa
     1201 cagggcgggg tccgcgtagc ccttgaagtc cgcgagcccg gcaccgaacg ccggggacgc
     1261 cacgaggaga aggacggcgc aggccaggtt gcgcgacagg ttcatcttgc agcctcctca
     1321 acggctcgag agcgacgatc cgatttgccg atcggaaatc atacgcgcgc ggcggggccg
     1381 ccggcccgac cgcgggccgg ccgtctcccg gcgtaccggg ccggcgaggt cgaaagctcg
     1441 cggcacctgg cgcctcgccg gcaccctccc cgacggagtc gccctggccc gtcaggaggc
     1501 cgcggcccct cccgcgcgct ggaagtcggc gaaggcgtgg tcggccgagg cgatgtgcga
     1561 aaggaggcgg cgcgcgaagg cgtcggcgtc ctcgcgggcc gcctcgtcga gcccgtccgc
     1621 gaggcgcgcc cggacgccct ggagctcccg gacgaacgcc gcgtggtcgg ccgcgtgggc
     1681 ctcggacgcg ggataggcgt ggaggcgcat gaggagctcc tcggcgagga agtgcgcgcg
     1741 cgtgaaggcc tcgagccgct cgaagacctc gagcgccgcg ggggcgtcgc ccgaatcgag
     1801 gttctcgacg agctcgcgga ggagggaaag ctgaagggcg tgctcctcgt ccatctgccg
     1861 gttcccggtg acgaggtccc ggtgtcgttc ggcggcggtc atcgtgcgtc cctccggagg
     1921 gtcctcaccc ggggacccag aggccgtccc gcacgacgat cggccttccg tcgatcgcga
     1981 cgcggcgccc gcagccgccg ggacggaagt cgcagacgac gtcgacgtgc tgcgccgagc
     2041 ggttcaggac gccgcgcgcc gcgagctcct cgagcctctc gcgggcgccg ggggccttcg
     2101 ggtcccgctc gaagcaggtc tcgtagctcg cgccgatcgc gacgtggagc gtcccgccca
     2161 ccttctcgac gaagagcggg tgcttcagcg cgcgcgtcag gccggggttc atgccgaggg
     2221 cgacctcgcc gaggcggcgc gccccctcgt ccgtcccgac gatcgaggcg agctgcgcct
     2281 ccccctcgag cgccgagaag cggacgatcc gtcctttctc gaagacgagg tgcatccccg
     2341 agacgtcgac gccgccgttc aggacgggga ggtcgaggaa gagctccccc tcgacggagg
     2401 aggcgtcggg gctcgtgaag acctcgccgt ccgggacgtt ccggaggccg tgggagagga
     2461 ccgggatcga ctcggcgagg ctcatctcga gcgtcagctc gcgcttctcg accgggtgga
     2521 acgtgacgac ctccatccgc ttcccgtcgg cgaagagcgg ctcgagccgc ttctccgcct
     2581 cgaggagcgg cagggggtcg accgtcgagg cgtcgacgac gaagcggacg tactcctcga
     2641 gcgggagccc ctcgatcccg gcgtaggcgg ggctcgggta gagggtgatg acccagcgct
     2701 tcgacaggcg gaggtcggcg aacgggcggt cggccttcgc gagcgcagcg gccgcctcgc
     2761 gcgagaggcc ggcgtagctc gacggcgact cggccccgag gacctcgatg tacttcgtca
     2821 tcgaggcgta gcggtcgcgg tgccagtcgg gaacgcgggc gagctgctcc gccgtcgcgc
     2881 cgcgccccat cgcggccgac cagaccttgc cgcgctcgtt gttcggcgcg acgaggcaga
     2941 cgtcggggat gccgccggcg gcgatcacct cccgctcgat ccgctccatg agcggccagg
     3001 cgatccgctc ccccttgatc atcacccggt cgtccggggt gacgccgccg atcgagtggg
     3061 tgacgaggag gcgggcccac tcgtcgagct tcgcggggtc gatcgtcggt tcgagcacgt
     3121 gtccctcctg gattccggcc ggcatcgatt cgtgcgctcc ggccgtccgg ttcccggcga
     3181 ccgaggatac cgaacggccc gccgggtcgg cttcttcgac cgccgctaca ggggcgtcac
     3241 cgtccagcgc gtcgtggagg cgctctcctg gccgaggccg gagctgtggc tctcctcgcg
     3301 cgcggacgcc gtgacgagcc gcccggccac ccgggtaaag cgccggctct cgcgcatgga
     3361 gacggtgaac gagacgagga gcttcttcgc cttcaggtcg agcgtcctct cgagggcgaa
     3421 cggcaggccg tcggcgtcga gccggagcgt gacgacgtcg tcgtagcgct tgatcgcctt
     3481 ccgggcctcg tcgtcgaggt cgggcacgac gcggaagacg atcgtccgcg cggtccttcc
     3541 ctcgaacgct tccgggcggt cggagacgac ggtcgccccg tccagcagct gccggagctc
     3601 cgtccccggg tcgacgagct ccagcgcatc ccccggctcg agccgcacgg tcttctcttc
     3661 gcgggccttc cgctccgcct tcgaccccgg cttcgggagg aaggccggat cgaggtggat
     3721 cgagagaccc gacggcccgt gctcgacgac cgcctcccca ctccgctcac ccttcggctt
     3781 ctccttctcg gagtcggttc gcgcgatcgc gacccggacc cggacggtct ccccggcggg
     3841 ggcgcggtcg agggcggccc gcaggtcggc gagggcgtcg gcccgggcgg gacggtgggc
     3901 gagcgcgagg ggcgagagga gggcgagggc gaatccggca gagaggacgg cacggcgcat
     3961 gcgagacggg gtacgcacac gccgtgccgc gggttcttct accgaaccgg gaacggcggc
     4021 cgcttcggcg cgaccggcgg gctcttcttc agctgctcca ggacgatctc gatcgccttc
     4081 tcgagctgcg ggtcgcggcc ggcgatgacg tcggccggcg tctgctcgac ctcgacgtcg
     4141 ggcgggacgc cgacgttctc gacgccgaag ccctcctcgg gcgcccagaa ggcgaggttc
     4201 ggggcggtga cctgcgcgcc gtccatcagg accgggaagc cgaggatccc gacgagaccg
     4261 ccccaggtcc gctggccgac gagcgggccg accttgaact tccggaacat ccacgggagg
     4321 aggtcgccgc ccgagccggc ggtctcgtcg atgagcatca ccttcggccc ctggatggag
     4381 gcgctcgggg tcttcatgtc gtcgccgtac cggaacgtcc accaggcaat cggctcgcgg
     4441 cggagcgcct cgatgtagta gtcggccacg ctgccgccgc cgttgaaccg ctcgtcgacg
     4501 atgatcgcgt ccttccacgc ctgcgggtag aagtagcgct tgaagtaggt gtgtccgagg
     4561 ccggccgtgt tcgggacgta gacgtacgcg acgcgcccgc ccgtcgcctt ctcgaccttc
     4621 gcgaggttgc cctcgaccca atcgcggttg cgaagggcgc cctcgtcggc caccgggacg
     4681 acgttcacgg tgcgtgagcc cttcccgtcg ggcgtcggcc cgacggtgat ttcgacgatc
     4741 tttccggcgg tcgcctcgaa gcgggagaag aggttctccg gcggcgcgag ctccttcccg
     4801 ttcaccgcga ggaggtactc gcccgccttc acctcgacgc ccggctcgct gagcggcgcg
     4861 cggagctcgg ggttccagtt caggccgccg aagaccttgc ggaagcgata gcgcccgctg
     4921 gccacctcgt agtcggcgcc gaggaggccg cccggaacgg gcgtcgcctc gtcgagccgg
     4981 tcgccgccga acgtccggtg gtggccgacg gcgagctcgc tgcacatcca ctggatgagg
     5041 cgcgtcaggt cggcgcgcga ggagaggtgc gggaggaagg gctcgtactt cgcccggatc
     5101 gccttccagt cgcggccgtg catcccgggg tcgtagaagt agtcgcggtt gatccgccag
     5161 acctcctcga gaatctgcgg ccactcggcg cgcgggtcga cgcgcacctg gacggcgtcg
     5221 agcttcagct tcccgttgcc ttcgcccggc ttcgcctcgc cccttttccc ggcggggccg
     5281 acctcgaagg attccttcgc gcgcgtcagc agcttcttcc cgtcgaagga gacctcgtag
     5341 tcgtcgacgt cggcggcgat cgtctcggcc ttccggctct tcgtgtccca cttctggagc
     5401 gccgtcttcc cgtcggcctc gcggaggaac cagacctcgc cggcgttcgg ggaggagagg
     5461 ccggagatgt cggcagcctt caccgggacg tcgacgatcc ggaggctgaa gccgtctcgg
     5521 tcgatgacga caggctcggg cttcttctcc tccttcttcg cgtcgtcctt cttcgcgtcc
     5581 ttcccgccct tgccgtccgc gggcttgccg tcctcgtcct tcttcccgtc cttggcggcg
     5641 tccttcccct cgcccttctc ctcgtcgctc tccttcgcga gcggcgacgg cgtgccggcc
     5701 ttgagcgtcg cgacgtagat cgcgcgggtg acgtccatgt cctggttgac gagcgagaac
     5761 cagttccgca ccgggccggc gtcggtcgag gcgaggaacc agatcagctt tccccccttg
     5821 tcgaagaccg ggtcggagac gtcggagagc ccgtcggtga gcgggaacga cttcccgtcg
     5881 acggtgtcgt ggacgtggag cgtgcgaatg taggtcgcgc ccgcgagcgt gtaggcgagc
     5941 cagcgggagt cgggcgacca gctcgccttc agcgtcttct gcggggcgta gagccgctcg
     6001 gaggcgacct tcaccgcctt cccggaggcg acgtcgatcc accagagggt ccaggagttg
     6061 tcggtgaggg cgatcttcgt cgcgtcgggg gaccaggcga ggcggtcgta gaagccggag
     6121 ccgggcacgg cgatcctctt cggagtcccc ttcccgtcct gcgcggcgac gacgaggcgg
     6181 tactcgcccc cttcgtcgga gacgtacgcg atcgacttgc cgtcgggcga ccaggccggc
     6241 ccgcgctcgt gcgcgccggg agtggccgtc aggtagcgcg ggtccccctt ctcggctggg
     6301 acggtgacga tctcgccccg gaactcgagc gcgacgcgcg cgccggacgg cgagagcgag
     6361 gcgtcgcgga cccactgcgc acccttcgcc cagcggggcc gggtctcgag gaggtcggcg
     6421 ttcaccgtga tcggaagcgg cgcggccttc ttcgtcgccg cgtcgaggag gtggaggagg
     6481 cccgcctgct cgtagacgac cttcccgttc gcggcggcgg cggcgaggac cgggaagtcc
     6541 tcgtgcgcgg tcaccttcgc gacgttcctc gacttcgcgt cgaacgtgta gaggttgagc
     6601 tcgccgtcgc ggtcggaggt gaagtagagc gtcccgccga cccacgtcgg gttcaggtcg
     6661 ttgcagcgcc cctcgggctg cgggaccttc acgatcgcgt gcgtcgcgac gtcgtagagc
     6721 cagatccgcg agacggtgcc gccgcggtag cgcttccact ggaggtgcgc ggggcggttc
     6781 ggcgtgtagg cgatcgtccc cccgtcgggg gagaagacgg cgcgggaggc gtacgggatc
     6841 gggagcttcg tctcgacgcc cccctcgacc ggcacggtgt agagctggtc gtgccggttg
     6901 ttcgtcgcct cgcgcgggga ggcgaagagg accgccttgc cgtcgggcgt gaagccctgg
     6961 acgacgtcgc gcgagggatg gaacgtgagg cgcttcggaa cgccgcccgt cgcggggacg
     7021 acgaagacgt cgaggttccc gtccagctcg gccgtgaacg cgaggagcgc gccgtcgggg
     7081 ctgaaggcgg ggctcgcctt cacgcccggg cccgacgtga tccgctggac gttcgagccg
     7141 tcgagccggg ccgtccagag gtcgttgccg tagacgaagg cgacgcgctc ggccgagagc
     7201 gccggctgcg tgagcatctt cgtcgggtct ccggcggagg cgggctgagg ggagaggacg
     7261 agggcgagga gggcgaagag ggcgagcagc ccggcggcac gaaggcgcat gcagtccctt
     7321 tccggccgca gcgacgcgcg gccgacggcg atgatagggg tgatacgggg ggcggggccc
     7381 gttcgttctc aggacgggga tcggggcgtt tcgccacacc acgacggcct acactccgag
     7441 acgtggtgac gacgcacaca cccgagtccc tctcctctct cgcgcgcggc ttcatggagt
     7501 cgcgcgtctt cctgacggcc gccgagctcg acctcttcac gctcctagcc gacgggccgc
     7561 tctcgctcga ggaggcggcg gcggcgcgcg gcgcggatcg gcgcgcgctc gcgatcctcc
     7621 tcgacgcgct gacggcgatg ggactcctcg agaaggcgga cgggcgtttc acgacgccgc
     7681 cgtcgctcgt gccgctcctg acctcgcgct cgggggggag cctcctgccg ctcgcgctcc
     7741 actccgtgaa cctctgggat cgctggtcgc ggctgacgga gaccgtcgcc ggggcgcgcc
     7801 ccgtcgccgg cgacggctgg acccgctctt tcatcggcgc catgcacgcc atcgcggcgc
     7861 ctcaggccga cgcgatcgtc gcggccgtcg gccccgggag cgcgcggcgg cttctcgacg
     7921 tcggcggcgg gccggccacg tacacgctcg cgttcctgcg ggccgccccg ggccttcgcg
     7981 cgacgctgtt cgaccttccc gaggtcgtgg agatcgcccg ggagaacgca acgcgggccg
     8041 gcctcgtcga ccgggtcgac ctcgtcgcgg gcgatctccg caccgacgcg cttcccggag
     8101 ggcacgacct cgcgttcgtc tcggcgatcg tccacatgct cggcccggcc gggaacgtcg
     8161 ccctcttccg gaaggtccat gacgcgctcc tccccggcgg gcgggtcgtt ctccgcgacc
     8221 acgtcatgtc gcccgaccgc acggccccgc gggccggggc tctcttcgcg gtgaacatgc
     8281 tcgtcgggac gaaggacgga ggcacgttca cgctcgagga gctctcggcg tggctcgagg
     8341 aggcggggtt tgtccggccg cgcctcctcc gggacggcga gcggatggac gcgctcgtgg
     8401 aggccgtgcg cccctgatcg ggcgagggag tccgcgggag cccgccgctc gccgcccgcc
     8461 tccccccggt gacgacggtc atcgtcgtgt cgaaaggagg gggcgcaccg tccccggcga
     8521 tggagattcg cccgcgcggg gaccatattc ccccgccaga caggaggcgg aacatggtcg
     8581 tcacggcgtc gctcttctcg tccgtcccgt ccgggccgtt ccagcccgag cacccgaacc
     8641 gcagcctcga agggattgcc tcggagatcc gccgggccta tccgcagggc gtcgacgtgg
     8701 tcgtcttcct gccggcagtt cagaacgggt ggagcctcgt cgcgaaccgg caggggatca
     8761 tcgcgatcct gatcggcctc ctcctcccgg cggtccagaa gg
//
LOCUS       DAOTEZ010000160         8737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5699_length_8737_cov_5.626008, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000160 DAOTEZ010000000
VERSION     DAOTEZ010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8737
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5699_length_8737_cov_5.626008"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="P5164_09815"
     CDS             complement(<1..910)
                     /locus_tag="P5164_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRY44230.1"
                     /translation="MAAPDLNLLVTLDVLLAEGSVARASRRLGLSASAMSRALARLRA
                     TTGDPLLVRAGRGLVPTPRAIALRDRVGPLVQEAEAMLRPAENIRLDTLERSFTLRSA
                     EGFVESFGASLLARVAREAPGVRLRFVQKLDRDNVPLRDANVDLETGVVDDTTDPEVR
                     VQALFRDRFVSVVRTGHPLCRGKVTPARYAAGWHVFVSRRGRSTGPMDDALKRLGLER
                     EIDTVVGGFSTALALARATDLIATVPERHTGSLRDGMHSFPLPFATPGFTVSMLWHPR
                     FHADAAHRWFRGCVKEVCEEQPGRATP"
     gene            1052..2503
                     /locus_tag="P5164_09820"
     CDS             1052..2503
                     /locus_tag="P5164_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY44231.1"
                     /translation="MHAIGDRRDEAARGEEERRVPVGWALASLSLPMLLSSLGTSIAN
                     VALPTLATSFRAPFHAVQWVVLAYLVATTTLVVSAGRLGDVTGRRRLLSAGILVFTLA
                     SALCGLAPTLGMLVAARALQGVGAATMLALTLAFVGETVPRERTGAAMGLLGTMSAVG
                     TALGPSLGGLLIAGLGWRAIFLVNLPLGAAALVMVRRFLPADRPAAKAGRARFDLAGT
                     VVLALTLASYALAMTVGRGGFGARGAALLAGAALGVALFVRAEARAKAPLVRVAAFRD
                     PALGAGFAMSALVSTVMMATMVVGPFYLARALGLDAGSVGIVLSAGPLVAAMTGVPAG
                     RAVDRFGAQRTMLAGLLGIVAGAVGLSMLPAAAGVVGYVVPIVVITAHYALFQTANNT
                     AVLADVDAGERGVVSGLLNLSRNLGLITGASVMGSVFAKASGAADVAAADPAALAAGM
                     RATFAVAAVLVVVALAVALASRAPAPSPAGEMS"
     gene            3112..3615
                     /locus_tag="P5164_09825"
     CDS             3112..3615
                     /locus_tag="P5164_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44232.1"
                     /translation="MKRRHARANGLASLLSAAGVLSTGCFSFPAMPLPGYAATETAPA
                     GVLGSFSVSSPLLGNVTVAPTGCVSGDLQRFLGGDFSDPASGLVVRLVVDPLEGPAAR
                     VFEAADPFRQSVVLRRSECAVFLFSLEHTGLTVNEVRDYRLSVELDCALADGTTVHGR
                     ASTTHCH"
     gene            3745..4545
                     /locus_tag="P5164_09830"
     CDS             3745..4545
                     /locus_tag="P5164_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44233.1"
                     /translation="MRRLNQGLPPSERVRVVGVDQPIWWDAIRTRSDYELFQDSLAGR
                     DFFLYATIRSRLDGLRKGEKAFFLTNTRHAYMRLRDARGRPQLNAAGFFARNDPGRAW
                     SVRLHNATLAVDGTVRTGGPRSMQGLESKSYRWVRMEDGAWDRAFAANGNVPVGLELA
                     GTPFGAAEFVGNTMLDAAPGSTMADAYDALIFLGPIDELRLSGTTGFYVTPEFQPELE
                     RRIRLVEGDGLARALAARGVSTVDALLDGLAAGTPARRNGLLDPPVKP"
     gene            complement(4571..5815)
                     /locus_tag="P5164_09835"
     CDS             complement(4571..5815)
                     /locus_tag="P5164_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44234.1"
                     /translation="MSAALPQPPGWGPGPPPPPAPGGTVSVRPLPDGEEIVLRATGWL
                     RLLPAAFLLFWLCGWAAGELLVLLILLGPLADPLVSALRDVLPGSWPRPPRFAGSTPW
                     PVLAFLAVWFVFWTWGGLAAAWQLLRLVAGSDRFVTRPGGFSLRPGAGPFGRTRDFPA
                     SEVSAVHADVRRSRLVAVVGGSERVLCSGTPLPVAEWLAARLRAALGLPEPTLADSGE
                     RDAEAFLEPPVPVDWAAGPLPEGGVLLSAPHARSRKTAGCALAVAVVVTAGFVAGLLA
                     AGVRREAPGGALFFGLLILAVDLLCGWLAFARESWLLRPGLIARVRSLGPWERRREVR
                     HGTLAVSVSTDSDDDDWFRLEACGEGRRLKLSSRINAGPEVLAFARYAARHSGFPLDV
                     PREVLLAAGELRNGSSRARGPA"
     gene            complement(5887..>8737)
                     /locus_tag="P5164_09840"
     CDS             complement(5887..>8737)
                     /locus_tag="P5164_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY44235.1"
                     /translation="LAEQQEPIVRRVAVKLLKAGMDTERVVARFEAERQALAMMDHPN
                     IARVVDAGATPSGRPFFVMDLVVGSPISTYCDENALSVAARLELFAQVCNAVQHAHTK
                     GIIHRDIKPSNVLVATHDGKPHAKVIDFGIAKATEARPAGETFFTVDGAMIGTPEYMS
                     PEQAEGSLDVDARTDVYSLGVLLYELLTGSTPFTTRELQAAGLAEIRRIIREVDPPKP
                     STRIGHSADTAGTSAAKRQTAPKRLSAIVRGELDWIVMRALEKDRQRRYPTANALAMD
                     VARYLSGEPVVAAPPGAAYRAGKFVKRHRAAVLGAAAVAAALVGGAIAFAWQARVARG
                     QRDRAVAAEARARTTADDLRKVSDFQAAMLAQVDPAAAGRMLAKEVHGQFERALAEAG
                     LPERERAAQRTAFSGQWGRVNATDAARNLIDQTILRPAVAAIDARFADQPAVDAQLRQ
                     VLADLYKTLGLYEAARPLQSRALETRRRVLGEASEGTLRSLGASGDLLRAEGKLAEAE
                     PILRDALERSRRALGAEHPFTLVSLNNLGYVLHLQGRNDDAERLCREALGTMQRVFGE
                     DHRETLATQGNLAQALQALGRLEEAEAVYRDSLARHRRALGEEAPETVVAVSNLANLL
                     LARGRFAEAEPVLREALAMSRRVLGEEHPDTVTAINNLGFSLQFQGKLEEAEPVEREA
                     VEKYRRAVGPDHPSTLAAMNNLALLLQDRRQYAEAEALLRETLAARRRVLGDEHPDTI
                     ACLGNLGRFLQAVDRLAEAEPVLRETLETKRRVRGPEHPDTITSINNYGAVLEAMGRP
                     AEAETYYRDAMEKCGRVLGPEHPNTFITMINTGGALQARGKAAEAEKVLDGVEAAARK
                     AFTGSNARWVAKLLTRRGMARTALGKLDAAEKDLLEAHPLLLSTRGEEHKETRECVKA
                     LVDLYRVRPGSEPGRGWDREAASWAAKLRPTARTR"
BASE COUNT         1103 a         3164 c         3262 g         1208 t
ORIGIN      
        1 cgggcgtcgc ccgccccggc tgctcctcgc acacctcctt gacgcagccg cggaaccagc
       61 ggtgcgcggc gtcggcatgg aaccgcgggt gccagagcat ggagacggtg aaccccggtg
      121 tggcgaacgg gagcgggaag ctgtgcatgc cgtcgcgcag gctccccgtg tgacgctcgg
      181 ggaccgtggc gatcaggtcg gtcgcgcgag ccagcgccag cgcggtcgag aagccgccga
      241 ccaccgtgtc gatctcgcgc tccagcccga gccgcttcag ggcgtcgtcc atcggccccg
      301 tgctccggcc ccgtcgcgag acgaagacgt gccagcccgc cgcgtaccgc gccggggtga
      361 ctttccccct gcacagcggg tgccccgtcc ggacgacgct gacgaaccgg tcgcggaaca
      421 gcgcctggac gcgcacctcc gggtcggtcg tgtcgtcgac gacccccgtc tcgagatcga
      481 cgttcgcgtc gcggaggggc acgttgtccc tgtcgagctt ctgcacgaag cgcagcctca
      541 cgccgggggc ctcccgggcg acgcgcgcga ggaggctcgc gccgaagctc tccacgaagc
      601 cctcggccga cctcagcgtg aagctccgct ccagcgtatc gaggcggatg ttctcggccg
      661 ggcgcagcat cgcctccgcc tcctgcacga gcggaccgac ccggtcgcga agggcgatcg
      721 ccctcggggt cgggacgaga ccccggcccg cccggacgag gagcggatct ccggtcgtcg
      781 cacgcagccg ggccagggct cggctcattg ccgaggcgct gagcccgagc ctccgggacg
      841 cgcgcgcgac gctcccctcg gcgagcagca cgtcgagcgt gacgaggagg ttgaggtccg
      901 gagccgccac gggcggaacg tagcacgaac cgacgcgaac atggcgtttc atgcatgtac
      961 tccatgcaac ccatgcgtct tccgccatac cgaaaacgcg cctaccttga tcgcagcacc
     1021 gaatccggaa ccagggaagg gaggactcac gatgcatgcg atcggcgaca gacgggacga
     1081 ggcggcgagg ggagaggagg aacgacgagt tccggtcgga tgggcgcttg cgagcctctc
     1141 gctgccgatg ctgctgtcgt ctctcggaac gagcatcgcc aacgtcgcgc tgccgacgct
     1201 ggcgacgtcg ttccgcgcgc cgttccacgc ggtgcagtgg gtcgtcctcg cctatctcgt
     1261 cgccacgacg acgctcgtgg tgagcgccgg gcggctggga gacgtcaccg gccgaaggcg
     1321 cctgctgagc gcggggatcc tcgtcttcac gctcgcctcg gccctgtgcg gcctcgcgcc
     1381 cacgctcggg atgctcgtcg cggcgcgggc gctgcagggc gtcggcgcgg cgaccatgct
     1441 ggcgctgacg ctggcgttcg tcggcgagac ggtcccccgg gagcggacag gggccgcgat
     1501 ggggctcctc ggaacgatgt cggcggtcgg cacggcgctc gggccctcgc tcggcgggct
     1561 cctgatcgcc gggctcgggt ggcgcgcgat cttcctcgtg aacctcccgc tcggcgccgc
     1621 cgctctcgtg atggtgcggc ggttcctgcc ggccgatcgt ccagcggcga aggccggtcg
     1681 ggcccgcttc gacctcgccg gcacggtcgt cctcgccctg accctcgcgt cgtacgcgct
     1741 cgcgatgacg gtggggcgcg gcgggttcgg agcgcgcggc gccgcgctcc tggcgggggc
     1801 ggcgctcggt gtggcgctct tcgtgcgggc cgaggcgcgg gcgaaggcgc cgctcgtccg
     1861 agtggcggcg ttccgggatc cggcgctcgg cgcaggcttc gcgatgagcg ccctcgtctc
     1921 gacggtgatg atggcgacga tggtggtcgg cccgttctac ctcgctcgag cgctcgggct
     1981 cgatgccggg agcgtcggga tcgtcctgtc ggcggggccg ctggtcgcgg cgatgaccgg
     2041 cgtgccggcg ggtcgtgcgg tcgatcgctt cggcgcgcag cgcacgatgc tcgcggggct
     2101 cctcgggatc gtggccggcg cggtgggcct gtcgatgctg ccggcagcgg ccggagtcgt
     2161 cgggtacgtc gtcccgatcg tcgtcatcac ggcccactac gcgctcttcc agacggcgaa
     2221 caacacggcc gtcctcgcgg acgtcgacgc gggcgagcgg ggcgtcgtct cggggctgct
     2281 caacctgtca cgcaacctcg gcctcatcac cggcgcgtcc gtgatgggat cggtcttcgc
     2341 gaaggcgtcc ggtgcggccg acgtcgccgc cgccgacccc gccgccctcg ccgcggggat
     2401 gcgggccacg ttcgcggtgg ccgcggtcct ggtcgtcgtc gccctcgccg tcgcgctggc
     2461 gagccgcgcg cccgcgccct cgccagccgg ggagatgtcg tgacgggtcc cgcgacgcgt
     2521 gtcggccggc gcggtccggc gggcggggcg cggaggcgtt ccggcgtgcg gcgagcgccg
     2581 ggctctctcc cccgagcgcc gcgggctggg gcccggaggc gggaggtggc tgcttcgtca
     2641 gccggtgggc cgaagaccgg accggcaggc gcgaggccgg agcgtcgccg ccgctgaagg
     2701 cgatggcgtt gtggggcggc gccgggccgc ggccgcgaac ctccgaatga cgcgtcgatc
     2761 ccgcagctct tcgtcgccgt tcgcgcaccc ccgtcatggt gccaggcgtg aaatcgtgta
     2821 gtgtttcaaa caatacacga tttcacgcct ggcaccacgc ttgtggcacc gcgcttgtgg
     2881 cgccgagctc gtcggccagc cgtcgctcgg cggtgcgcgg tcgagcgaca ggaacgatga
     2941 ggccggctcg agacattccc tttcgttgga tcagcccgag gatgttcgag gcgacgttcg
     3001 gcctgcgacg gagcggcgcg acccgcactt cgggccgggg aggcggggaa cgaccggagc
     3061 gcgaggagcg tcacggaacc ggtgccgagg tccgtgaaag actcgtccgc catgaaacgg
     3121 cggcacgccc gggcgaacgg tctcgcgagc ctcctctctg cggcgggcgt cctgtcgacc
     3181 ggctgcttct cgttccccgc gatgccgctc cccggctacg ccgcgacgga gacggctccc
     3241 gcgggagttc tcgggtcctt ctcggtgagc tcgcccctcc tcgggaacgt gaccgtcgct
     3301 cccacgggct gcgtcagcgg ggacctgcag cgcttcctcg gaggggattt ctccgacccc
     3361 gcctcgggtc tcgtcgtccg cctcgtcgtc gaccccctcg aggggcccgc cgcgcgcgtc
     3421 ttcgaggccg ccgacccgtt ccggcagagc gtcgtcctgc gacgctccga gtgcgccgtc
     3481 ttcctcttct cgctcgagca cacggggctg acggtcaacg aggtccgcga ctaccgtctc
     3541 tccgtcgagc tcgactgcgc gctggcggac ggaacgaccg tccatggccg cgcctcgacg
     3601 acgcactgcc actgagccgg cgccggcgcc acccgccgga ggcctgcatc acgggacgac
     3661 cggagcctcc tcgccccggc gttccgcgac gacttctcgg gctacggctg gcgttactcc
     3721 acctatctcg agctgctggc caccgtgcgg cggctgaacc aggggcttcc gccgtccgag
     3781 cgcgtgcgcg tcgtcggcgt cgaccagccg atctggtggg acgccatccg gacgcgatcc
     3841 gactacgagc tcttccagga ctctctcgcg ggccgcgact tcttcctcta cgcgacgatc
     3901 cgaagccgcc tcgacggcct ccgcaagggg gagaaggcgt tcttcctcac gaacacgcgc
     3961 cacgcgtaca tgcgactgcg cgatgcccgg gggcgtccgc agctcaacgc cgccgggttc
     4021 ttcgcgcgca acgaccccgg gcgcgcctgg tcggtgcgcc ttcacaacgc gacgctcgcc
     4081 gtcgacggga ccgtacggac cggcggtccg cgctcgatgc aggggctgga gtcgaagtcg
     4141 tatcgctggg tgcggatgga agacggggcc tgggaccgtg ccttcgcggc caacggcaac
     4201 gtccccgtcg gcctcgagct tgcgggcacg ccgttcggcg ccgcggagtt cgtcgggaac
     4261 acgatgctcg acgcggctcc cggatcgacg atggccgatg cctacgacgc gctgatcttc
     4321 ctcggaccga tcgacgagct gcgtctctcg ggcacgaccg gcttctacgt gacgccggag
     4381 ttccagccgg agctggagcg ccgcatccgg ctcgtggaag gcgacggtct cgcccgcgcc
     4441 ctcgccgccc ggggcgtctc gacggtcgac gccctcctcg acggcctcgc cgccgggacg
     4501 cccgcgcgaa ggaacggtct cctcgatccg ccggtgaagc cgtagccgtc gccggcagag
     4561 gcggaccggc tcacgccgga ccccgcgctc tcgagcttcc gttccgcagc tcgccggccg
     4621 cgagaagcac ctcgcgcggc acgtccaggg ggaatcccga gtgccgggcc gcgtagcgcg
     4681 cgaaggcgag gacctccggc ccggcgttga tccggctcga cagcttcaac cgccggccct
     4741 cgccgcacgc ctccaggcgg aaccagtcgt cgtcgtccga atcggtcgac acggacaccg
     4801 cgagcgtacc gtgacgcact tcgcgccggc gctcccaggg gccgaggctc cgcactctcg
     4861 cgatgaggcc gggacgaagg agccacgact cgcgcgcgaa cgcgagccag ccgcagagga
     4921 ggtcgacggc gaggatcagg agcccgaaga agagggcgcc tcccggggct tcgcggcgaa
     4981 cgccggcggc gaggagcccg gcgacgaagc cggccgtaac gacgacggcg acggcgagcg
     5041 cgcagccggc ggtctttcgc gacctcgcgt ggggcgccga gaggaggacg cctccctcgg
     5101 ggagcggccc ggccgcccag tccaccggga ccggaggctc gaggaaggct tcggcgtccc
     5161 gctctcccga gtccgcgagt gtcggttcgg ggaggccgag ggcggcccgg aggcgggcgg
     5221 cgagccactc cgccacgggc agcggggtcc cggagcagag gaccctctcc gagcctccga
     5281 cgaccgcgac gaggcgcgag cggcggacgt cggcgtgaac ggccgagacc tccgaggcgg
     5341 ggaagtccct cgtccggccg aaggggcctg cgccgggcct cagggagaag ccgcccgggc
     5401 gggtgacgaa ccggtcactc cccgcgacga gccgaaggag ctgccaggcc gccgccagcc
     5461 cgccccacgt ccagaagacg aaccagaccg cgaggaaggc caggaccggc cacggcgtcg
     5521 agcccgcgaa ccggggcggc cgcggccagg accccgggag cacgtcgcgc agcgcggaga
     5581 cgagcgggtc ggcgagcggg ccgaggagga tgaggaggac gaggagctct cccgcggccc
     5641 agccgcagag ccagaagaga aggaatgcgg ccggcaggag gcggagccag cccgtcgccc
     5701 gaaggacgat ctcttcgccg tccgggagag gacgcaccga gacggtcccg cccggagccg
     5761 gcggcggggg aggcccgggc ccccacccgg gcggctgcgg cagcgcggcg gacacggcgg
     5821 catcgtagcg cccggcggcg aggaccacgc gacgcaccgg tcgttcggga ccgagggtcc
     5881 gggggtctac cgggtgcggg ccgtcgggcg gagcttcgcg gcccaggacg cggcctcgcg
     5941 gtcccagcct cggcccggct ccgaacccgg acggacccga tagaggtcga cgagcgcctt
     6001 cacgcactcg cgcgtctcct tgtgctcttc gccgcgcgtc gagaggagca aggggtgggc
     6061 ctcgaggagg tccttctccg cggcgtcgag cttcccgaga gccgtccgcg ccattccgcg
     6121 ccgcgtcagg agcttggcca cccatcgcgc gttcgacccg gtgaaggcct tgcgggccgc
     6181 ggcctcgacg ccgtcgagga ccttctcggc ctcggcggcc ttcccgcggg cctggagggc
     6241 gccgcccgtg ttgatcatcg tgatgaacgt gttggggtgc tccggaccga ggacacgccc
     6301 gcacttctcc atcgcgtccc ggtagtacgt ttccgcctcg gccgggcgcc ccatcgcctc
     6361 gaggacggcg ccgtagttgt tgatcgaggt gatcgtgtcg ggatgctcgg ggccgcggac
     6421 gcggcgcttc gtctcgaggg tctcgcggag gaccggctcc gcctccgcga gccggtccac
     6481 ggcctggagg aaccggccca ggttgccgag gcaggcgatc gtgtcagggt gctcgtcgcc
     6541 gaggacgcgc cggcgcgccg cgagcgtctc tctcaggagc gcctcggcct cggcgtactg
     6601 gcgccggtcc tggagcagga gagcgaggtt gttcatcgcg gcgagcgtgc tcgggtggtc
     6661 ggggccgacg gcccgccggt acttctcgac cgcctcgcgc tcgaccggct ccgcctcctc
     6721 cagcttcccc tggaactgga gggagaagcc caggttgttg atcgccgtca ccgtgtcggg
     6781 gtgctcctcg ccgagcaccc ggcgggacat cgcgagcgcc tcgcgcagga ccggctcggc
     6841 ctcggcgaac cgcccccggg cgaggaggag gttcgcgagg ttgctgacgg cgacgacggt
     6901 ctcgggagcc tcctcgccga gcgcgcggcg gtgccgcgcg agggagtcgc ggtagaccgc
     6961 ctcggcctcc tcgagccggc cgagcgcctg cagtgcttgg gccaggttcc cctgagtcgc
     7021 cagcgtctcg cggtggtcct cgccgaagac gcgctgcatc gtcccgagag cttcccgaca
     7081 gagccgctcg gcgtcgtcgt tccggccctg caggtggagg acgtagccga ggttgttgag
     7141 ggagacgagc gtgaacgggt gctccgcgcc gagggcgcgg cggctcctct ccagcgcgtc
     7201 gcgcaggatc ggctcggcct cggccagctt cccctcggcg cgcaggaggt cgcccgacgc
     7261 cccgagcgag cggagcgtcc cctcgctcgc ctcgccgagg acccgccggc gcgtctcgag
     7321 cgcccggctc tggagcggtc tcgcggcctc gtagaggccg agggtcttgt agaggtcggc
     7381 gaggacctgc cggagctgcg cgtcgacggc cggctggtcc gcgaagcgcg cgtcgatcgc
     7441 ggcgaccgcg ggccggagga tcgtctggtc gatcaggttc cgcgccgcgt cggtggcgtt
     7501 gacccgtccc cactggccgg agaacgccgt ccgctgcgcc gccctctccc gctccgggag
     7561 ccccgcctcg gccagcgccc tctcgaactg cccgtggacc tccttcgcga gcatccggcc
     7621 cgcggcggcc gggtcgacct gcgcgagcat cgccgcctgg aagtcggaga ccttgcggag
     7681 gtcgtccgcc gtcgtgcgcg cccgggcctc cgccgccacg gcgcggtcgc gctggccgcg
     7741 tgcgaccctc gcctgccagg cgaacgcgat ggcgcccccg acgagcgcgg cggcgaccgc
     7801 ggcggccccg aggacggcgg cccggtggcg cttcacgaac ttcccggcgc ggtacgcggc
     7861 gcccggaggc gcggcgacga ccggctcgcc gctcaggtag cgcgccacgt ccatcgccag
     7921 ggcgttcgcc gtcgggtagc ggcgctgccg gtccttctcg agcgcgcgca tgacgatcca
     7981 gtcgagctcg ccgcggacga tcgccgagag gcgcttcggg gccgtctgcc gcttcgcggc
     8041 ggaggtacct gccgtgtcgg cggagtggcc gatgcgggtc gacggcttcg gagggtcgac
     8101 ctcgcggatg atccgccgga tctcggcgag gccggcggcc tgcagctcac gcgtggtgaa
     8161 gggcgtcgac cccgtcagga gctcgtagag gaggacgccc agggaataga cgtcggtccg
     8221 cgcgtcgacg tcgagcgagc cttcggcctg ctccgggctc atgtactcgg gcgtgccgat
     8281 catcgcgccg tccacggtga agaaggtctc gcccgccggc cgggcctccg tcgccttcgc
     8341 gatgccgaag tcgatgacct tcgcgtgcgg cttcccgtcg tgggtcgcga cgaggacgtt
     8401 cgacggcttg atgtcgcggt ggatgatccc cttcgtgtgg gcgtgctgga cggcgttgca
     8461 gacctgcgcg aagagctcga ggcgcgccgc gacggagagc gcgttctcgt cgcagtaggt
     8521 cgagatcggc gaccccacga cgaggtccat gacgaagaag gggcgaccgc tcggcgtcgc
     8581 gccggcgtcg acgacgcgag cgatgttggg gtggtccatc atcgcgagcg cctgccgctc
     8641 ggcctcgaag cgcgcgacga ccctctccgt gtccatcccg gccttcagga gcttcacggc
     8701 gacgcggcgg acgatcggct cctgctgctc ggcgagg
//
LOCUS       DAOTEZ010000161         8721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5711_length_8721_cov_6.761712, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000161 DAOTEZ010000000
VERSION     DAOTEZ010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8721
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5711_length_8721_cov_6.761712"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="P5164_09845"
     CDS             <1..552
                     /locus_tag="P5164_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44236.1"
                     /translation="TSTPTSTPTNTPTFTPTSTPTSTPTSTPTSTPTPTVTPTTTPTV
                     VPTAPPAVDLGETTAVPGQALFASGSVPDTSLSYQLCLIANATWTLGTPFGGVCTLSA
                     PLTSPTSAFSGAPLGPAPGAGSYDLLLLGPASTVLAADDASSAPGLVVTGLADVPVGG
                     RAGLLGFAFLIAAAAWVLLRQFR"
     gene            complement(629..1549)
                     /gene="ftcD"
                     /locus_tag="P5164_09850"
     CDS             complement(629..1549)
                     /gene="ftcD"
                     /locus_tag="P5164_09850"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HRY44237.1"
                     /translation="MTTLIECIPNISEGRRPEVVEAVRKAAVSAGPGVLLLDWTSDAD
                     HNRSVLTFLGDGPGLLRGMEAMVDEAMRHVDLTKHEGAHPRLGAVDVIPFVPIRGATT
                     ADCVALAKELGARLASKHGLPIYLYEDAAASEARRNLATIRKGEFEGLAEKMKSPEWK
                     PDYGPDAPHPTAGATVVGARSPLIAYNINLGTADLSIADRIAKSIRHLSGGFRFVKAM
                     GVRLEARGIVQVSINMTNYEKTPLHRVFETVRSEAERFGVPVVGSEIVGLVPQAALVG
                     AAEHYLRLEAPASSQVLENKLLDVAFAGSQ"
     gene            1651..3624
                     /locus_tag="P5164_09855"
     CDS             1651..3624
                     /locus_tag="P5164_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44238.1"
                     /translation="MPELAAYPGSPALSAEAREKVLQTFRHTLEMARAGRNEDALLGC
                     DFILKMDARFAPARQLLASLRGIAAGTVVDLADFAPYLGGGEPAAQAPPATPPSGYGV
                     PPPPAEAAPAVDPFAAPGAGLDSLSFDDLAANPFAPAPSHDPFAAPPPAADFSFGDPA
                     PAASPAADPFAAAPALDAFAPAAAAPDPFAAPAPAAFPSAPPAASGVDPRIAQFLRQG
                     DEAEARGALQEAIDLWSRVFLIDLSNEDASRRIDAARARQDEAARQLDVLLSEGVQLY
                     EAGDLTSARDAFLKVLALSESDATARNYLNEIDAALASTAGAAAPAQAVPHAPVFSHA
                     PAPAAPVEPYLRDELESPGRPSFADGLPSLDETPIGEERLAPVAPAPAEAPAAAGGKG
                     GKVDGRILVGAAALVLVGVAALAWFLFRPKSDAPSADLPGGGAPVSAAPAVDPIEKAR
                     ALFKEGKAEEALSLLTAVPTTDPRHDQALVLIDTIKSSAQPGPAAASTVSDAEVDAAR
                     LAGFASLESGRYIDAVKSLDVVVRARQDDTEAAQALVKARDAVSALGSAIRSYNEQDY
                     ESAQRLLWDLRKQDPKNQDVEEYLFKSYFNDGIMGLQAGSMKKAAESFREAAALRPRD
                     ADTQRHLQFARKYPNGSNDLLSRIYVRHVTPRP"
     gene            3635..4270
                     /locus_tag="P5164_09860"
     CDS             3635..4270
                     /locus_tag="P5164_09860"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRY44239.1"
                     /translation="MTEKKHDPLAEHPLGGEAAPPPRGRGKTLSLFPDVSEAPFGPEP
                     AATEAAAPPSAAEAGAPPPDDVDEPGPMVDEPDTAVTFARRFAAGLADVVILGLFGAL
                     ELAAGAILLELRFPPSALLGVGAFLLLAAFILLVLVPFVWGATPGMALADLRVAANDG
                     GSPTLSAAMLRFVGALLTGALAGVPLLVAAFDRKGRTLSDLLSRTTLVAAR"
     gene            4304..5983
                     /locus_tag="P5164_09865"
     CDS             4304..5983
                     /locus_tag="P5164_09865"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HRY44240.1"
                     /translation="MRVALGQMNATVGDFSGNGAKIRALWKEAEAARADLLLVPEMAL
                     CGYPARDLLERPGFLHEAARLLDDLARRSGKTALLAGTVLPNPAKTGKPLFNAAVLLR
                     NGRVAAVARKSLLPAYDVFDEVRHFEPASRPTLARLAGRKVGLTICEDLWNDKDFWRA
                     RRLYATDPGETLVARGADLILNVAASPFSEGKPRLRRRMLARLARDGGLPVVYLNLVG
                     GNDELVFDGGSMVLDGNGRVRARAALFEEDFVVVDVDGPRVAPVSGSEPADLPDDAGG
                     EPLESLRRALVLGIRDYARKCGFRTAVVGLSGGIDSALVGALAVDALGAENVTGLALP
                     SRYSSEGSVADARALASALGIRFEVVSIEPLFAAARATLAPLFAGRPEDVTEENLQAR
                     LRGMLLMAFSNKLGPLLLTTGNKSELAVGYCTLYGDMAGGLAPISDLPKMRVYELSRH
                     LNRRAGRELIPASTLEKAPSAELRPDQTDQDSLPPYALLDRILEGLVAGNLPPAKVAR
                     ETGAPEALVAGLARQIDRAEYKRQQAAPGLKVSTKAFGSGRRIPIAQVPGT"
     gene            5980..6681
                     /locus_tag="P5164_09870"
     CDS             5980..6681
                     /locus_tag="P5164_09870"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRY44241.1"
                     /translation="MKYAGELPRSRYPWKEIPGSSHAVLLGRIATLGDSLSVLDVGFG
                     AGHLARRIRPRCRYLAGIELDPEAAREGSAFFDDPVLDDVVRGLSGPWRESFDVVVAA
                     DILEHLPEPGRALDLLRPLLGGDGLLLVSLPNVANVTVRLALLLGRFPRAERGILDRT
                     HLGFFTRRTGRELLEAHGFRVVRETPTAMPVELALPLLGRPPLAAPVRAAAALLARAW
                     PGMFGYQFVYEARPV"
     gene            6678..7430
                     /locus_tag="P5164_09875"
     CDS             6678..7430
                     /locus_tag="P5164_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY44242.1"
                     /translation="MTRPPALSLVVPAYNEEKRLPVSLARISEWVLSRSPQLSVEVLV
                     VDDGSSDRTAAVAEKTAAGLGLDFRVLRLPRNRGKGAAVREGVLAAGGEQVLVTDADL
                     STPIEEVDKLLAAGAPVAIGSRAVDETLVKQRQSLFRVASGKLFNLLVRLLAVSGIHD
                     TQCGFKLFRADAAREVFSRATVDRFAFDVEALLLARRLGYPIAEVPVLWFNSPDTRVG
                     LGGGLEAFAALFRIRRRVAQTLRERPPAPRAP"
     gene            7495..8160
                     /gene="elbB"
                     /locus_tag="P5164_09880"
     CDS             7495..8160
                     /gene="elbB"
                     /locus_tag="P5164_09880"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HRY44243.1"
                     /translation="MKPKVGVVLSGCGVYDGAEIHESVLTLLALDRAGSEAVCLAPDV
                     EQKHVVNHLTGQPADGESRNVLVEAARIARGKVKSLAGFDPASLDALVLPGGFGAAKN
                     LCDFAFRGADCEVHPDVARVVRAVHAAGKPVGAICIAPVILARLLGGEKPKLTIGTDP
                     GTAAAIGKMGGEHVSCGGGKAVVDEEKRLVTTPAYMLDSPISEVSAGIEKLVAELLAM
                     MRR"
     gene            complement(8168..>8721)
                     /locus_tag="P5164_09885"
     CDS             complement(8168..>8721)
                     /locus_tag="P5164_09885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012598417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY44244.1"
                     /translation="GPGTRQVLEIGSGNSTKFLRHLIGALGLTTRIVSIDPFPRAECD
                     ALCDTVIREPLETVDLALFDDLEPGDVVFFDGSHRCFMNSDVTAFFLDALPRIPSGVA
                     IGVHDIFLPDDYPEEWAERYYSEQYVLAAWLLGAEGRYRVTLPAYWVSRNRERLGLDA
                     IADSFGCPRERFDGCSFWIEKTG"
BASE COUNT         1110 a         3477 c         2913 g         1221 t
ORIGIN      
        1 acgtccaccc cgaccagcac gcccacgaac accccgacgt tcacgccgac cagcaccccc
       61 accagcacgc ccacgagcac cccgacgtcc acgccgacgc cgaccgtcac gcccaccacg
      121 acgccgacgg tcgtccccac ggcgcctcct gccgtcgatc tcggagagac cacggccgtc
      181 cccggccagg ccctgttcgc ctccgggagc gtccccgaca cctccctcag ctaccagctc
      241 tgcctgatcg cgaacgcgac gtggacgctc gggaccccct tcggcggcgt ctgcacgctc
      301 tcggcgcccc tgacgtcccc gacgagcgcg ttcagcggcg cgccgctcgg ccccgccccg
      361 ggagccggga gctacgacct gctgctcctc gggcccgcct cgacggtcct cgcggccgac
      421 gacgcctcgt ccgcgcccgg cctcgtcgtc accgggctcg cggacgtccc cgtcggcggg
      481 cgagccggcc tgctcggctt cgcgttcctg atcgcggccg cggcctgggt cctcctccgg
      541 cagttccgct gagcccgagc cctcgcgccc gccggggcgc ctcgccctcc gggcaggcgg
      601 cttcccgccc gcccggagcc gtaccggcct actggcttcc cgcgaacgcg acgtcgagga
      661 gcttgttctc gaggacctgg gaagaggcgg gggcctcgag ccggaggtag tgctcggcgg
      721 ccccgacgag cgccgcctgg gggacgaggc cgacgatctc ggagccgacg accgggaccc
      781 cgaagcgctc ggcctcggag cgaaccgtct cgaagacccg atggagcggc gtcttctcgt
      841 agttcgtcat gttgatcgag acctggacga tcccgcgcgc ctcgagccgg acgcccatgg
      901 ccttcacgaa ccggaagcct cccgagaggt gccggatcga cttcgcgatc cggtcggcga
      961 tcgaaagatc ggccgtcccg aggttgatgt tgtaggcgat gagcggggag cgcgcgccca
     1021 cgaccgtcgc gcccgccgtc gggtgcgggg cgtccggtcc gtagtcgggc ttccactcgg
     1081 ggctcttcat cttctcggcg agcccctcga actccccttt ccggatcgtc gccaggttcc
     1141 gccgcgcctc gctcgccgcg gcatcctcgt acaggtagat cggcaggccg tgcttcgacg
     1201 cgagccgcgc gccgagctcc ttcgcgagcg cgacgcagtc ggccgtcgtg gcgccgcgga
     1261 tcgggacgaa ggggatcacg tcgacggcgc cgaggcgcgg gtgcgcgccc tcgtgcttcg
     1321 tcaggtcgac gtggcgcatc gcttcgtcga ccatcgcctc catcccgcgg aggagcccgg
     1381 gcccgtcgcc caggaaggtc aggacggagc ggttgtggtc ggcgtcggag gtccagtcga
     1441 ggaggaggac ccccggcccc gcagagacgg cggctttccg gacggcctcg acgacctccg
     1501 gccgccgccc ctcgctgatg ttcgggatgc actcgatcag tgtcgtcatg gcaacctctc
     1561 cccgttcggg ggccgaagat cataccgtgc ggccgtcagg gcgcttgccg ccggaaagac
     1621 ggcttgttac gctgacttcg agaggttcgc atgcccgagc tcgctgccta tccagggagt
     1681 cccgctctct ccgccgaggc ccgcgagaag gtcctccaga cgttccgcca cacgctcgag
     1741 atggctcggg ccggccggaa cgaggacgcg ctcctcggct gcgacttcat cctgaagatg
     1801 gacgcgcgct tcgccccggc gcgccagctc ctcgcctcgc tccgggggat cgccgcgggg
     1861 accgtcgtcg acctcgccga cttcgcgccc tacctgggcg gcggcgagcc cgccgcccag
     1921 gcgcctccgg ccacgccgcc gtccggctac ggcgttccgc cgccgcccgc ggaggccgcg
     1981 cccgccgtcg atccgttcgc cgccccgggc gccgggctcg acagcctgag cttcgacgac
     2041 ctcgcggcga acccgttcgc tcccgcgccg tctcacgacc cgttcgccgc cccgccgccc
     2101 gccgccgact tctccttcgg ggatccggcg ccggccgctt cgcccgcggc cgatccgttc
     2161 gccgccgccc cggctctcga cgccttcgcc cccgccgcgg cggctcccga cccgttcgcc
     2221 gcgcccgctc ccgccgcgtt cccctccgcg ccgccggccg cgtccggagt cgacccccgg
     2281 atcgcgcagt tcctgcgcca gggggacgaa gcggaggccc ggggcgccct gcaggaggcg
     2341 atcgacctct ggagccgcgt cttcctgatc gacctctcga acgaagacgc ctcacggcgg
     2401 atcgacgcgg cccgcgcacg gcaggacgag gcggcgcgac agctcgacgt cctcctctcc
     2461 gagggggtcc agctttacga ggccggagac ctcacctcgg ctcgggacgc cttcctgaag
     2521 gtcctggcgc tctccgagtc ggacgcgacg gcgcggaact acctcaacga gatcgacgcg
     2581 gcgctcgcct ccacggccgg ggcggccgcc cccgcccagg ccgtcccgca cgcccccgtc
     2641 ttctctcacg ctcctgcgcc cgccgccccg gtcgaaccct acctgcgcga cgagctcgag
     2701 tcgccggggc gtccctcctt cgcggacggg cttccctccc tcgacgagac gccgatcggg
     2761 gaggaacgcc tcgcaccggt cgcacccgct ccggccgagg cgcccgcggc cgccggcggc
     2821 aagggtggaa aggtggacgg gcggatcctc gtcggcgcgg ccgcgctcgt tctggtgggc
     2881 gtcgcggcgc tcgcctggtt cctcttccgg ccgaagtcgg acgcgccctc ggccgacctc
     2941 cccggcggag gcgcccccgt gtcggctgcg ccggcggtcg acccgatcga gaaggcccgg
     3001 gcgctcttca aggaagggaa ggccgaggag gccctctccc tcctcacggc cgtcccgacg
     3061 accgacccgc ggcacgacca ggcgctcgtc ctcatcgaca ccatcaagtc gtccgcgcag
     3121 ccggggccgg ccgcggcctc gaccgtcagc gacgccgagg tggacgccgc gcgcctcgcc
     3181 ggcttcgcga gcctggaatc cggccgctac atcgacgccg tcaagtcgct cgacgtcgtc
     3241 gtccgcgcgc ggcaggacga caccgaggcg gcccaggccc tcgtgaaggc ccgggacgcc
     3301 gtctccgccc tcgggtcggc gatccgctcg tacaacgagc aggactacga gagcgcccag
     3361 cggctcctct gggaccttcg caagcaggac ccgaagaacc aggacgtgga ggagtacctc
     3421 ttcaagagct acttcaacga cgggatcatg gggctccagg cgggcagcat gaagaaggcg
     3481 gcggagtcgt tccgggaagc ggccgccctc cgcccgaggg acgccgacac gcagcggcac
     3541 ctgcagttcg cccgcaagta cccgaacggg tcgaacgacc tcctgtcccg gatctacgtc
     3601 cggcacgtca cgccgcgccc ctgacggccc ggacatgacc gagaagaagc acgacccgct
     3661 cgcggagcat ccgctcgggg gcgaagccgc gcctcctccc cgcggacgcg ggaagacgct
     3721 gagcctcttc cccgacgtct ccgaggcgcc gttcggaccc gagcccgcgg cgacggaggc
     3781 cgcggccccg ccttccgccg cggaggcggg cgcgcccccg ccggacgacg tcgacgaacc
     3841 cggcccgatg gtcgacgagc ccgacacggc cgtgaccttc gcgcggcgat tcgccgccgg
     3901 gctcgccgac gtcgtcatcc tcggcctctt cggagcgctc gagctggcgg ccggagcgat
     3961 cctcctcgag ctccgcttcc cgccctccgc cctcctcggt gtcggcgcct ttctcctcct
     4021 ggccgccttc atcctcctgg tcctcgttcc cttcgtctgg ggggcgacgc ccggaatggc
     4081 ccttgccgac ctgcgcgtgg cggccaacga cggcggctcg ccgacgctgt ccgccgcgat
     4141 gctccgattc gtcggggcgc tcctgaccgg agccctcgcc ggcgtcccgc tcctcgtcgc
     4201 ggcgttcgac cggaaggggc gcaccctctc cgacctcctc tcgcgcacga ccctcgtcgc
     4261 ggctcgctga gagccggccg cggcgagggt tacgatccgc ccgatgcgcg tcgccctcgg
     4321 ccagatgaac gccaccgtcg gcgacttctc cggcaacgga gcgaagatcc gggcgctctg
     4381 gaaggaggcc gaggcggcga gggccgacct cctcctcgtc cccgagatgg cgctctgcgg
     4441 ctacccggca cgcgacctcc tcgagcgccc cggattcctt cacgaggcgg cgcggctcct
     4501 cgacgacctt gcgcggcgct ccggaaagac cgccctcctc gccggcaccg tcctcccgaa
     4561 cccggcgaag accggcaagc cgctcttcaa cgcggccgtc ctcctgcgaa acggccgtgt
     4621 cgccgccgtc gctcgcaaga gcctcctccc cgcctacgac gtcttcgacg aggtccgtca
     4681 cttcgagccg gcgagccgcc ccacgctcgc ccggctcgcg gggcgtaagg tggggctgac
     4741 gatctgcgag gacctctgga acgacaagga cttctggcgt gcgcgccgcc tctacgcgac
     4801 cgatccgggc gagacgctcg tggcgcgcgg ggccgacctg atcctcaacg tcgccgcctc
     4861 cccgttctcg gaggggaagc cgcgcctgcg gcgccgcatg ctcgcgcgcc tcgcgcgaga
     4921 cggcgggctg ccggtcgtct acctgaacct cgtcgggggc aacgacgagc tcgtcttcga
     4981 cggcggctcg atggtcctcg acgggaacgg ccgcgtgcgg gcgcgtgcgg cgctcttcga
     5041 ggaggacttc gtcgtcgtcg acgtggacgg cccgcgcgtc gcccccgtct ccggctcgga
     5101 gccggccgac ctccccgacg acgccggcgg cgagccgctc gagtcgctcc ggagggccct
     5161 cgtcctcggc atccgcgact acgcgcgcaa gtgcggcttc cggacggcgg tcgtcggcct
     5221 ctccggcggg atcgactccg cgctcgtcgg cgcgctcgcc gtcgacgccc tcggcgcaga
     5281 gaacgtcacc ggtctcgcgc tcccctcccg ctactcctcg gaggggtcgg tcgccgacgc
     5341 gcgcgccctc gcctcggcgc tcggcatccg cttcgaggtc gtctccatcg agccgctctt
     5401 cgcggctgcg cgggcgacgc tcgcgccgct cttcgccggc aggcccgagg acgtgaccga
     5461 ggagaacctc caggcgcgcc tgcgcgggat gctcctgatg gccttctcga acaagctcgg
     5521 accgctcctc ctgacgacgg gcaacaagag cgagctggcc gtcggctact gcacgctcta
     5581 cggcgacatg gccggcgggc tcgcgccgat ctcggacctt ccgaagatgc gcgtctacga
     5641 gctctcccgg cacctgaacc ggcgcgccgg acgcgagctg atccccgcct cgaccctcga
     5701 gaaggcgccg tcggccgagc tccggccgga ccagaccgac caggactcgc tccctcccta
     5761 cgccctcctc gaccgcatcc tggagggcct cgtcgccggg aacctccctc ccgcgaaggt
     5821 ggcgcgcgaa accggagccc ccgaggcgct cgtcgcgggc ctcgcccggc agatcgaccg
     5881 ggccgagtac aagaggcagc aggccgcccc cggcctgaag gtctccacga aggcgttcgg
     5941 cagcggacgc cgcatcccga tcgcgcaggt ccccgggacg tgaagtacgc gggcgagctg
     6001 ccccgttcgc gctacccgtg gaaggagatc cccggctcct cgcacgcggt cctcctcggg
     6061 cgcatcgcca cgctcggcga ctctctctcg gtcctcgacg tcggcttcgg cgccgggcac
     6121 ctcgcccgcc gcatccgccc acgttgccgc tacctcgccg ggatcgagct cgacccggag
     6181 gcggcgcggg agggctcggc gttcttcgac gacccggtcc tcgacgacgt cgtccggggc
     6241 ctctccggcc cctggcgcga gtcgttcgac gtcgtcgtcg ccgccgacat cctcgagcac
     6301 cttcccgagc cgggccgcgc gctcgacctc ctgcggccgc tcctgggcgg cgacggcctc
     6361 ctcctcgtct cgctcccgaa cgtcgcgaac gtcacggtcc gcctcgcgct cctcctcggg
     6421 cgtttcccgc gggcggagcg cggcatcctg gaccggacgc acctcggttt cttcacccgc
     6481 cgcacgggac gggagctcct cgaggcgcac ggcttccggg tcgtccggga gaccccgacc
     6541 gcgatgcccg tcgagctcgc ccttcccctc ctcggccgac ctcctctcgc cgcccccgtc
     6601 cgcgccgccg cggcgctcct cgcgcgcgcc tggcccggga tgttcggcta ccagttcgtc
     6661 tacgaagcga ggcccgtttg acccgaccgc cggccctgtc cctcgtcgtc cccgcctaca
     6721 acgaggagaa gcgcctcccc gtctccctcg cgcggatctc cgagtgggtc ctctcgcgat
     6781 cgccgcagct ctccgtggag gtcctcgtcg tcgacgacgg ctcctcggac cggaccgccg
     6841 ccgtcgcgga gaagaccgcg gccggcctcg gcctcgactt ccgcgtcctt cgcctgcccc
     6901 ggaaccgcgg gaaaggagcg gccgtccgcg agggggtcct cgccgccggc ggggagcagg
     6961 tcctcgtcac cgacgccgac ctctcgaccc cgatcgagga ggtcgacaag ctcctcgccg
     7021 ccggggcccc cgtggcaatc ggctcccgcg ccgtcgacga gacgctcgtc aagcagaggc
     7081 agtcgctctt ccgcgtcgcg agcgggaagc tcttcaacct cctcgtccgc ctcctcgccg
     7141 tctcgggcat ccacgacacc cagtgcggct tcaagctctt ccgcgccgac gccgcgcggg
     7201 aggtcttcag ccgggcgacc gtcgaccgtt tcgcattcga cgtcgaggcg ctcctcctcg
     7261 cgcgccggct cgggtatccg atcgcggagg tgccggtcct ctggttcaac tcgcccgaca
     7321 cccgggtcgg cctcggcgga ggcctcgagg ccttcgccgc gctcttccgg atccggcgcc
     7381 gcgtcgccca gacgctgcgc gagcgccctc cggccccgcg cgccccctga cgaccggggc
     7441 ggttccctct ccgcgccgcg gccgatacga ttcgcctccg gacggaggtg aaccatgaag
     7501 ccgaaagtcg gtgtcgtcct ctcgggctgc ggcgtctacg acggcgccga gatccacgag
     7561 tcggtcctga cgctcctcgc gctcgaccgc gccggctccg aggcggtctg cctcgccccc
     7621 gacgtcgagc agaagcacgt cgtgaaccac ctgaccgggc agccggcgga cggcgagagc
     7681 cgcaacgtcc tcgtcgaggc ggcccgcatc gctcgcggca aggtgaagag cctcgccggc
     7741 ttcgacccgg cgtcgctcga cgccctcgtc ctgcccggcg gcttcggggc ggcgaagaac
     7801 ctctgcgact tcgcgttccg cggagccgac tgcgaggtcc accccgacgt cgcccgcgtc
     7861 gtccgcgccg tccacgccgc cggcaagccg gtcggcgcca tctgcatcgc gccggtgatc
     7921 ctcgcgaggc tcctcggcgg cgagaagccg aagctgacga tcggcaccga cccggggacg
     7981 gccgcggcga tcgggaagat ggggggcgag cacgtctcct gcgggggagg gaaggccgtc
     8041 gtcgacgagg agaagcgcct cgtcacgaca cccgcctaca tgctcgacag cccgatctcc
     8101 gaggtctcgg ccggcatcga gaagctcgtc gcggagctgc tcgcgatgat gcgccgctga
     8161 cagcgcttca gccggtcttc tcgatccaga acgagcagcc gtcgaagcgt tcgcgcgggc
     8221 agccgaacga gtcggcgatc gcgtcgaggc cgagccgctc gcgattccgg gagacccagt
     8281 acgcgggaag cgtcacgcgg taccgccctt ccgcaccgag gagccaggcg gcgaggacgt
     8341 actgctcgct gtagtagcgc tcggcccact cctcggggta gtcgtcggga aggaagatgt
     8401 cgtggacccc gatcgcgacg ccgctcggga tccgcggaag ggcgtcgagg aagaatgctg
     8461 tcacgtcgct gttcatgaag cagcgatggc tcccgtcgaa gaagacgacg tctcccggct
     8521 cgaggtcgtc gaagagagcg aggtcgaccg tctcgagcgg ctcgcgaatc accgtatcgc
     8581 acagggcgtc gcactccgcg cgcgggaaag ggtcgatcga gacgatccgc gtcgtcagcc
     8641 cgagcgcccc gatgaggtgc cggaggaact tcgtggaatt gcccgagccg atctcgagca
     8701 cctgccgcgt acccggtccg a
//
LOCUS       DAOTEZ010000162         8609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5802_length_8609_cov_6.485504, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000162 DAOTEZ010000000
VERSION     DAOTEZ010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8609
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5802_length_8609_cov_6.485504"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1975
                     /locus_tag="P5164_09890"
     CDS             <1..1975
                     /locus_tag="P5164_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY44245.1"
                     /translation="TAVVEGLARRIASGDVPESLKDKQVVSLDLGAMLAGAKYRGEFE
                     ERLKAVIKEVEESEGKVILFIDELHTLVGAGAAEGAMDAGNMLKPALARGELRAIGAT
                     TLGEYRKHVEKDPALERRFQPVLVGEPSVEDTISILRGLQERYEVHHGVKITDAAIVA
                     AAVLSNRYIADRFLPDKAIDLIDEAASRLKMEIDSLPTAIDEVERKVLQAEIEKKALE
                     KDDSRSARERLEALEKALAELREKSLSMKAVWKNEKDAIGRIRDLKKKIEDAKADAER
                     AERQGDLSRAAELRYGVLNQLDRDLAAATEKLEDLQKDGGFLREKVEEEDVAAVVSRW
                     TGIPVSKMVEGETQKLLAMEERLRLRVVGQDEAVSAVSAAVRRARAGMNDPKKPIGSF
                     LFLGPTGVGKTELARALAEFLFDDEAAMVRLDMSEYQEKHTVSRMIGAPPGYIGHDEG
                     GQLTEAVRRRPYSVVLFDEIEKAHPDVWSTLLQVLDDGRLTDGKGRAVDFRNVVVVMT
                     SNVGSQLIQELQGVDEEEMKKGVEAELRHRFRPEFLNRIDDVIVFRRLTLEEIGRIVD
                     LQLARVAALVAPRGVKLEWTDAARRLLAERAFDPLYGARPLKRNIQKLVQDPLALRIL
                     SGDVPNGSTVSVDAGASGEGVVFSIGNPAAPAA"
     gene            1999..2475
                     /locus_tag="P5164_09895"
     CDS             1999..2475
                     /locus_tag="P5164_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRY44246.1"
                     /translation="MAAVRRYDPFRELATLQERMNRIFDDTFSSASRREDEGLAATWA
                     PPVDVLEKRDRIVLTAELPGFSEDQVQLRFEDGVLTLEGERRFEKEVDEARYHCVERS
                     YGRFSRSFRLPANVDEEAITATFVNGLLVVELPKREEARPKSIRIRAGSPPPIDVK"
     gene            complement(2486..3451)
                     /gene="cysK"
                     /locus_tag="P5164_09900"
     CDS             complement(2486..3451)
                     /gene="cysK"
                     /locus_tag="P5164_09900"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRY44247.1"
                     /translation="MRIASDVTKLIGNTPLVRLNRMAKDIAPGAVVAAKLEYFNPANS
                     VKDRIGVAMIEALEKEGRIVPGRTVLVEPTSGNTGIALAFVAAAKGYRLVVAMPESVS
                     LERRKVLRLLGAELILTPAAEGMKGAIARANERLAELGENGVMPQQFENPANPAIHEA
                     TTAVELWNDTDGSMDVFVAGVGTGGTMTGVGRFWRPKKPSVRLVAVEPVHSPVLSGGA
                     PGPHRIQGIGAGFVPANLDTALVDEVVGVTNDEAFEHARRLAREEGILGGISSGAAAC
                     AALRIAKRPENAGKLVVFVVPSTSERYISTDLFKEDEAGSANGAA"
     gene            complement(3483..3812)
                     /locus_tag="P5164_09905"
     CDS             complement(3483..3812)
                     /locus_tag="P5164_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44248.1"
                     /translation="MAALLALSRESVHQLLAPYVRAGLLVAVRGRTGGYRAGAGLLES
                     PLSSVLAVYGAPSGRARAAEKGLSRIVEAVESEAAAARFAVYERWTVGDLVARHRADR
                     HVIDWEI"
     gene            3949..5265
                     /locus_tag="P5164_09910"
     CDS             3949..5265
                     /locus_tag="P5164_09910"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRY44249.1"
                     /translation="MSRSTIAAAVLLLSATSAFAQRGAPEVRRAEDDPRRSPIVRAVE
                     QIGPSVVNISAERLVQRRATPFQEFFGVPGDRGRRSESLGSGVVLDASGIVVTNDHVV
                     SGASRIFVTTSDGRELEAEVLGADADNDLAVLKVDGKGLKPVRLGTTGDLMIGEAAIA
                     VGNPFGLSNTVTTGIVSALQRTVRGETRTYTDFIQTDAAINPGNSGGALCNVLGELIG
                     INTAIVGGANTIGFAIPVERVRRIVDDLLRFGEVKPVWVGVRGTTVTPERGRPSSRGL
                     GMRVRSVYPASPAEEAGLEPGDVVVSLNGRPVESREDFDTLLASVPPGQSVSLEARRS
                     GELRKLSLAVARAPEDLGLEVLRREIGISVAQARGGLFVTSVARESPADRKGLERGDV
                     LVAVNGRRVSTLEDVARAVERGYGRSGLVLVVGRGGYAYTLTFGLE"
     gene            complement(5315..5644)
                     /locus_tag="P5164_09915"
     CDS             complement(5315..5644)
                     /locus_tag="P5164_09915"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRY44250.1"
                     /translation="MECKSLRESGEELRAFDVAYFAASVDPLEQITKFAKSLDADYPM
                     LADADGSVAKAYGVRHPVMGFPRRWTFYIGRDGKLLHVDRDVKAGTAGPDMAKRLAEL
                     GIAKTGK"
     assembly_gap    5694..5793
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5794..6699)
                     /locus_tag="P5164_09920"
     CDS             complement(<5794..6699)
                     /locus_tag="P5164_09920"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY44251.1"
                     /translation="MSAPDLPRREALLADSALVLVTAIWGSTFVVNELVLKETPPLLF
                     LALRYGLAAAVLVLLARGRPRTPGVLRDGALIGLLLAVGIGCQLVGQLFTTASKAAFI
                     TGLSVPLTPVAAWLFHRQVPSGTNLAGLVLAAGGFGLMSWPKDASGLDPGDLLVFFTA
                     LVYAVIIVELGVASPKHDVRVYSAAQIVFAFLGVLAGRLLLTPFLGRPEAFFAAEARP
                     LAFTTQLLFAVLWMGLVASVVTSLVQTWAQARMPAVHAAVIFALEPVFTALFAALTLG
                     ERLSAREAWGGGLVLLGIVVSELRIR"
     gene            6752..8179
                     /gene="gltX"
                     /locus_tag="P5164_09925"
     CDS             6752..8179
                     /gene="gltX"
                     /locus_tag="P5164_09925"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRY44252.1"
                     /translation="MTPAPRVRFAPSPTGSLHVGGARTALYNWLFARRHGGTFILRIE
                     DTDVDRNRPELVDQILEAMTWLGMTWDEGPFHQSERTHLYREAAERLLSEGKAYRAFE
                     TPEELEAARKAAEAQGETFRYSGAGREIPKAESDRRAAAGEPFVLRLKMPDEPIVVDD
                     VVQGRAEFPAGALDDFVLTRSDGHPLYHFSVCVDDVAMGITHVIRGVDHLANTPKHVA
                     LFRALGAEAPKFAHLGMILGPDGKKLSKRHGAAGAEEFRAQGLLPEALVNFLALLGWS
                     PGEDRERMTMDEMAALFSLERIGPSPSRFDHEKLAWLNGQYLQDVPAGRLADLVKPFG
                     VPAGCSDETLLRAIPLQKPRAKTLVELAQGLSSWVADPADWDAAGLKKFGKPANAPLL
                     KTFLERIAAIEPLTHAGIEEAARALAAEAGVKLGDLAQPVRLALTGTLASPPLFEMIE
                     VLGLETTRRRVLALAARIESPEAPS"
     gene            8188..>8609
                     /locus_tag="P5164_09930"
     CDS             8188..>8609
                     /locus_tag="P5164_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012479204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRY44253.1"
                     /translation="MEETTTPAPETAGKDFIREIVEDDLKSGKHAKIATRFPPEPNGY
                     LHIGHAKSICLNFGVAREYGGTCNLRYDDTNPLKEDVEYVDAIEADVRWLGFGWNGKY
                     YASDYFGRLYDFAEELIRRGKAFVCDLTADEVRATRGTL"
BASE COUNT         1329 a         3040 c         3001 g         1139 t
ORIGIN      
        1 gacggccgtg gtcgaggggc tcgcgcggcg catcgcctcc ggtgacgtcc ccgagagcct
       61 gaaggacaag caggtcgtct ctctcgacct cggtgcgatg ctggccgggg cgaagtaccg
      121 gggcgagttc gaggagcggc tgaaggcggt gatcaaggag gtcgaggagt cggaggggaa
      181 ggtcatcctc ttcatcgacg agctccacac gctcgtcggc gccggcgccg ccgagggggc
      241 gatggacgcc gggaacatgc tcaagcccgc gctcgcccgc ggcgagctgc gggccatcgg
      301 cgccacgacg ctcggcgagt accgcaagca cgtggagaag gaccccgcgc tcgagcggcg
      361 cttccagccc gtcctcgtcg gcgagccctc ggtcgaggac acgatctcga tcctccgcgg
      421 cctgcaggag cgctacgagg tccaccacgg cgtgaagatc accgacgcgg cgatcgtcgc
      481 ggcggcggtc ctctcgaacc gctacatcgc cgaccgcttc ctccccgaca aggcgatcga
      541 cctgatcgac gaggccgcct cgcggctgaa gatggagatc gactcgctcc cgaccgccat
      601 cgacgaggtg gagcggaagg tcctccaggc cgagatcgag aagaaggcgc tcgaaaagga
      661 cgactcgagg agcgcccgcg agcggctcga ggcgctcgag aaggcgctcg ccgagctgcg
      721 ggagaagtcg ctctcgatga aggcggtctg gaagaacgag aaggacgcga tcgggaggat
      781 tcgcgacctg aagaagaaga tcgaggacgc gaaggccgac gccgagaggg ccgagcggca
      841 gggcgacctg agccgcgcgg ccgagctccg ctacggggtg ctcaaccagc tcgaccgcga
      901 cctcgccgcg gcgacggaga agctcgagga cctccagaag gacggcggct tcctccgcga
      961 gaaggtcgag gaggaggacg tcgccgccgt cgtctcccgc tggaccggca tccccgtctc
     1021 gaagatggtc gagggggaga cgcagaagct cctcgcgatg gaggagcgcc tccggctcag
     1081 ggtcgtcggg caggacgagg ccgtctcggc ggtctctgcg gccgtgaggc gcgcccgcgc
     1141 cgggatgaac gacccgaaga agccgatcgg ctcgttcctc ttcctcggcc cgacgggcgt
     1201 cgggaagacc gagctcgccc gcgccctcgc cgagttcctc ttcgacgacg aagccgcgat
     1261 ggtgcggctc gacatgtcgg agtaccagga gaagcacacc gtctcgagga tgatcggcgc
     1321 ccctcccggc tacatcgggc acgacgaggg ggggcagctg acggaagcgg tccgccgccg
     1381 gccgtactcg gtcgtcctgt tcgacgagat cgagaaggcg caccccgacg tctggtcgac
     1441 gctccttcag gtgctcgacg acggccgcct caccgacggc aaggggcgcg ccgtcgattt
     1501 ccggaacgtg gtcgtcgtga tgacctcgaa cgtcggctcg cagctcatcc aggagctgca
     1561 gggcgtcgac gaggaggaga tgaagaaggg ggtcgaggcg gagctgagac accgcttccg
     1621 gcccgagttc ctgaaccgga tcgacgacgt catcgtcttc cgccgcctca cgctcgagga
     1681 gatcggacga atcgtcgacc tccagctcgc gcgcgtcgcc gcgctcgtcg ccccgcgcgg
     1741 cgtgaagctg gaatggaccg acgccgcgcg acgtctcctc gccgagcggg ccttcgaccc
     1801 gctctacggc gcccggccgc tgaagcggaa catccagaag ctcgtccagg acccgctcgc
     1861 gctcaggatc ctctccggag acgtcccgaa cgggtccacc gtctccgtcg acgccggcgc
     1921 ctcgggcgag ggcgtcgtct tctccatcgg gaacccggcc gcacctgcgg cctgacgaaa
     1981 gcaagcaggg aggagaccat ggccgccgtt cgtcgatacg accccttccg cgagctcgcg
     2041 accctccagg agaggatgaa ccggatcttc gacgacacgt tctcgagcgc ctcgcgccgc
     2101 gaggacgagg gcctcgcggc gacctgggcg ccgccggtcg acgtcctcga gaagagggac
     2161 cggatcgtcc tgaccgccga gctccccggc ttctcggaag accaggtcca gctccgcttc
     2221 gaggacgggg tcctgacgct cgagggggag cggcgcttcg agaaggaggt cgacgaggcc
     2281 cgctaccact gcgtggagcg gagctacggc cgcttctccc gctccttccg gcttccggcg
     2341 aacgtggacg aggaggcgat cacggcgacc ttcgtgaacg gcctcctcgt cgtcgagctg
     2401 ccgaagcggg aagaggcccg gccgaagagc atccggatcc gcgccggcag cccgccgccg
     2461 atcgacgtga agtgagcccg gcggctcagg ccgccccgtt cgcggaaccc gcctcgtcct
     2521 ccttgaagag gtccgtcgag atgtagcgct cggaggtcga cggcacgacg aagacgacga
     2581 gcttccccgc gttctccggg cgcttcgcga tccggagggc ggcgcaggcg gcggcgccgg
     2641 aggagatccc gccgaggatc ccctcttcgc gcgcgaggcg ccgggcgtgc tcgaaggcct
     2701 cgtcgttcgt gacgccgacg acctcgtcga cgagggcggt gtcgaggttc gccgggacga
     2761 agccggcgcc gatcccctgg atccggtgcg gcccgggcgc tccgcccgag aggaccggcg
     2821 agtggacggg ctcgaccgca acgaggcgga cggacggctt cttcggcctc cagaaacggc
     2881 cgacgcccgt catcgttccc cccgtgccga cgccggccac gaagacgtcc atggagccgt
     2941 ccgtgtcgtt ccagagctcg accgcggtcg ttgcctcgtg gatcgcgggg ttcgccgggt
     3001 tctcgaactg ctgcggcatg acaccgttct cgccgagctc ggcgaggcgc tcgttcgccc
     3061 gggcgatcgc ccccttcatc ccctccgcgg ccggcgtcag gatcagctcc gcgccgagga
     3121 gacggaggac tttccggcgc tcgagggaga ccgactccgg catcgcgacg acgagccggt
     3181 accccttcgc ggcagccacg aaggccagcg cgatccccgt gttccccgag gtcggctcga
     3241 cgaggacggt ccggccgggg acgatccgcc cctccttctc cagcgcctcg atcatcgcca
     3301 cgccgatccg gtccttcacg gagttggccg ggttgaagta ctccagcttc gcggcgacga
     3361 ccgcgccggg cgcgatgtcc ttcgccatcc ggttcaggcg gacgaggggt gtgttgccga
     3421 tgagcttcgt gacgtcggac gcgatccgca tggcggcctc ctggactccg ggttccccgg
     3481 cgtcaaatct cccagtcgat gacgtggcga tcggcgcggt gccgggcgac gaggtcgccg
     3541 acggtccacc gctcgtagac ggcgaatcgc gccgcggcgg cctcggactc gaccgcctcg
     3601 acgatccgtg aaagcccctt ctccgccgcg cgggcgcggc cggacggagc gccgtacacc
     3661 gcgagaacgg aagagagcgg cgactcgaga agccccgccc cggcccgata ccctccggtc
     3721 cgcccacgga ccgcgacgag caggcccgcg cggacgtacg gcgcgaggag ctggtggacc
     3781 gattctcgcg aaagggcgag cagggctgcc atttcggcgg cgctccggtc ccgattcggg
     3841 tccgatccga tctcgaccat cgcctcgatc gcctttcttt gcctgaggtt gagaatcttg
     3901 aaatctccgt tctactttac taacattagt ttgaggccga atctcaaaat gtcaagatcc
     3961 accatcgccg ctgccgtcct tctcctttcc gccacgtcgg ctttcgccca gcggggggcc
     4021 cctgaggtcc gtcgcgccga ggacgatccc cgcaggagcc cgatcgtgag ggcggtcgag
     4081 cagatcggac cgtccgtcgt gaacatctcg gccgagcgcc tcgtccagcg gcgggcgacg
     4141 ccgttccagg agttcttcgg cgtccccggc gaccgcggcc gccgctccga gtcgctcggc
     4201 tccggcgtcg tcctcgacgc gtccgggatc gtcgtcacga acgaccacgt cgtctcgggc
     4261 gcctcccgca tcttcgtgac gacctccgac ggccgcgagc tcgaggcgga ggtcctcggc
     4321 gcggacgccg acaacgacct cgcggtcctg aaggtcgacg gaaaggggct gaagccggtc
     4381 cgcctcggga cgacgggcga cctgatgatc ggcgaggcgg cgatcgccgt cgggaacccg
     4441 ttcggccttt cgaacacggt gacgaccggg atcgtctccg ccctccagcg gaccgtccgg
     4501 ggagagacgc gcacctatac ggacttcatc cagaccgacg cggcgatcaa cccggggaac
     4561 tcgggcggcg cgctctgcaa cgtcctcggc gagctgatcg gcatcaacac ggcgatcgtc
     4621 ggcggcgcga atacgatcgg cttcgcgatc ccggtggagc gggtccgccg gatcgtcgac
     4681 gacctcctcc ggttcggcga ggtgaagccg gtctgggttg gggtccgcgg gacgacggtg
     4741 acgccggagc gcgggcgtcc gagctcgcga gggctcggga tgcgcgtccg ctccgtctac
     4801 cccgcctctc ccgccgagga ggccggcctc gagcccggcg acgtcgtcgt ctcgctgaac
     4861 ggccgccccg tcgagtcgcg cgaggacttc gacacgcttc tcgcctccgt cccgcccggc
     4921 cagtcggtct cgctcgaggc gcgccgctcc ggcgagctcc gcaagctctc cctcgcggtc
     4981 gcccgcgccc cggaggacct cggtctggag gtgctccgcc gggagatcgg catctcggtc
     5041 gcgcaggccc gcggtggcct gttcgtcacg tcggtggctc gcgagtcacc ggcagatcga
     5101 aagggcctcg aacgcggcga cgtcctcgtc gcggtgaacg gccgaagggt ctcgaccctc
     5161 gaggacgtcg cgcgcgcggt cgagcgcggc tacgggcggt ccggcctcgt cctcgtcgtc
     5221 gggcgcggcg gctacgccta cacgctgacg ttcggcctcg agtgagacgg aagcggagcg
     5281 ggccgccgca cggcggcccg ctccggccct cgcgtcactt ccccgtcttc gcgatcccga
     5341 gctcggcgag ccgcttcgcc atgtcgggtc ccgccgttcc cgccttcacg tcgcggtcca
     5401 cgtggagcag cttcccgtcc ctgccgatgt agaacgtcca tcggcgcggg aaccccatca
     5461 ccgggtgacg gacgccgtag gccttcgcga cggagccgtc ggcgtcggcg agcatcgggt
     5521 agtcggcgtc cagggacttc gcgaacttcg tgatctgctc cagcgggtcg acgctcgccg
     5581 cgaagtaggc gacgtcgaac gcccggagct cctcgccgct ctcacggagc gacttgcact
     5641 ccagcgttca ccctccggtg aagaccttcg ggaaccaggc gaggacgacg gccnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccggatc cggagctccg agacgacgat
     5821 cccgaggagg acgaggccgc ccccccaggc ctcccgcgcc gagagccgct cgccgagcgt
     5881 cagcgccgcg aagagggccg tgaagaccgg ctcgagagcg aagatgaccg ccgcgtggac
     5941 ggccggcatc cgggcctgcg cccaggtctg gacgagcgag gtgacgaccg aggcgacgag
     6001 ccccatccag aggacggcga agaggagctg cgtcgtgaac gccaggggcc gcgcctcggc
     6061 cgcgaagaag gcctccgggc ggccgagaaa cggcgtgagg aggagacggc ccgcgaggac
     6121 gccgaggaag gcgaagacga tctgcgcggc cgagtagacc cgcacgtcgt gcttcggcga
     6181 ggcgacgccg agctccacga tgatcacggc gtagacgagc gccgtgaaga agacgaggag
     6241 gtcgcccggg tcgaggcccg aggcgtcctt cggccacgac atcagcccga agccccccgc
     6301 cgccaggacc agccccgcga ggttcgtccc gctcggcacc tggcgatgga agagccacgc
     6361 ggcgaccggg gtcagcggca ccgagagccc cgtgatgaag gcggccttcg aggccgtcgt
     6421 gaagagctgg ccgacgagct ggcagccgat gccgacggcg aggaggagcc cgatgagcgc
     6481 gccgtcccgg aggacgccgg gcgtccgcgg ccggccgcgc gcgaggagga cgaggaccgc
     6541 cgcggcgagc ccgtaccgga gcgccaggaa gaggagcggc ggcgtctcct tgaggacgag
     6601 ctcgttgacg acgaacgtgg agccccagat cgccgtcacg aggacgaggg ccgagtcggc
     6661 gaggagcgcc tcgcggcgcg ggaggtccgg agcgctcacg gccggcgatg atacgggtcg
     6721 gcgggagcgg cgcagttatc atcaccgacc gatgactccg gccccgcgcg tccgcttcgc
     6781 cccctccccg accggctcgc tccacgtcgg cggcgcccgc accgccctct acaactggct
     6841 cttcgcccgc cggcacggcg ggacgttcat cctccggatc gaggacaccg acgtcgaccg
     6901 gaacaggccg gagctcgtcg accagatcct cgaggcaatg acctggctcg ggatgacctg
     6961 ggacgagggg ccgttccacc agagcgagcg gacccacctc taccgggagg cggccgagag
     7021 gctcctttcc gaggggaagg cctaccgcgc gttcgagacc cccgaggagc tcgaggcggc
     7081 ccgcaaggcg gccgaggcgc agggcgagac gttccgctac tcgggcgccg ggcgcgagat
     7141 cccgaaagcc gagtcggacc ggcgcgccgc ggcgggcgag ccgttcgtcc tccggctgaa
     7201 gatgcccgac gagccgatcg tcgtcgacga cgtcgtccag gggcgggccg agttcccggc
     7261 cggcgccctc gacgacttcg tcctcacgcg ctccgacggc cacccgctct accacttctc
     7321 ggtctgcgtc gacgacgtcg cgatggggat cacgcacgtc atcaggggcg tcgaccacct
     7381 cgcgaacacg ccgaagcacg tcgccctctt ccgcgcgctc ggcgccgagg cgccgaagtt
     7441 cgcccacctc gggatgatcc tcggccccga cgggaagaag ctcagcaagc ggcacggcgc
     7501 ggcgggcgcc gaggagttcc gcgcgcaggg gctcctcccc gaggcgctcg tcaacttcct
     7561 cgccctcctc ggctggtcgc cgggcgagga ccgcgagcgg atgacgatgg acgagatggc
     7621 cgcgctcttc tcgctcgagc ggatcggccc ctcgccttcg cgcttcgacc acgagaagct
     7681 cgcctggctg aacggccagt atctgcagga cgtccccgcg gggcggctcg ccgacctcgt
     7741 gaagccgttc ggagtcccgg ccggctgttc cgacgagacg ctccttcgcg cgatcccgct
     7801 ccagaagccc cgcgcgaaga cgctcgtcga gctcgcgcag gggctctcct cgtgggtcgc
     7861 cgacccggcc gactgggacg ccgccgggct gaagaagttc ggcaagcccg cgaacgcgcc
     7921 gctcctgaag acgttcctcg agcggatcgc ggcaatcgag ccgctgaccc acgccgggat
     7981 cgaggaggcc gcccgagccc tcgccgccga ggcaggcgtg aagctcggcg acctcgccca
     8041 gccggtccgc ctcgcgctga ccggcaccct cgcctccccg cccctcttcg agatgatcga
     8101 ggtcctcggc ctcgagacga cccgccggcg cgtcctcgcg ctcgcggcgc ggatcgagag
     8161 cccggaggcc ccgagctgag gcgcccgatg gaagagacga cgacccccgc ccccgagacc
     8221 gccggcaagg acttcatccg cgagatcgtc gaggacgacc tgaagtcggg gaagcacgcg
     8281 aagatcgcga cccgcttccc gcccgagccg aacggctacc tccacatcgg ccacgccaag
     8341 tcgatctgcc tcaacttcgg cgtcgcgagg gagtacggcg gcacctgcaa cctccgctac
     8401 gacgacacga acccgctgaa ggaggacgtc gagtacgtcg atgccatcga ggccgacgtg
     8461 cggtggctcg gcttcggctg gaacgggaag tactacgcct cggactactt cgggaggctc
     8521 tacgacttcg ccgaggagct gatccggcgc gggaaggcgt tcgtctgcga cctgacggcc
     8581 gacgaggtcc gcgccacgcg cgggacgct
//
LOCUS       DAOTEZ010000163         8576 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5837_length_8576_cov_6.257247, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000163 DAOTEZ010000000
VERSION     DAOTEZ010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8576
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5837_length_8576_cov_6.257247"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="P5164_09935"
     CDS             complement(<1..303)
                     /locus_tag="P5164_09935"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRY44254.1"
                     /translation="MSLELVVFAIASGLAIVSALAMVVVKTPVRSVVSLLVTFFALAV
                     LFVLLAAPFIAAIQVIVYAGAILVLFLFVVMLLNLTQEAEGHDERPIQRLLGLVSVV"
     gene            complement(317..1351)
                     /gene="nuoH"
                     /locus_tag="P5164_09940"
     CDS             complement(317..1351)
                     /gene="nuoH"
                     /locus_tag="P5164_09940"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HRY44255.1"
                     /translation="MSDATFALVATGAKILFVWVIALATILPNLVWAERRVAGMIQDR
                     PGPNRVGPFGFFQIIADGTKFFMKESVTPLYVDKLLYNLAPWITLMPGLVTFAVIPFG
                     ATLPVTIGGVTREVPLVIADVNIGILYIFAFTGLGIYGIVLAGWSANNKYSLMGGLRA
                     SAQIISYELAMGFAAAGVFLAAGTLRLSGVVEWQQQHVWNVVPQFVGFLVFLIAAFAE
                     TNRLPFDLAEAEEELAGGFHAEYSSFKFGMFFMGEYANLIAASALLATLYFGGWSLPG
                     VAFTGVWGALASVAVFFAKTIGFIFLFLWVRWTFPRFRYDQLMDLGWKFLLPLSFFHL
                     IAVAAFVAAK"
     gene            complement(1348..3060)
                     /locus_tag="P5164_09945"
     CDS             complement(1348..3060)
                     /locus_tag="P5164_09945"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRY44256.1"
                     /translation="MAKVTVDGRTVEVPDSTNVLEACRAAGVDVPHFCYHPRLSVVGQ
                     CRMCMVEIEGVPKIQASCTVPVRDGMVVLASSEKALAARNATMEFLLINHPLDCPICD
                     QAGECKLQDNAVAAGLPVSRTAEPRRQFPGYDRTSIGPHVIADMTRCIQCTRCIRFCQ
                     EIAGTGELTFIERGGHTIVWTHEGKELANDWSGCAADVCPVGALTTKEFRFRKRVWWL
                     DKARSVCDGCEIGCNVSIEHRDRTVYRFLPRVNLAVNDFWMCDYGRFRAEELNGNDFT
                     RPVLRKDGSARETNWGEALDAVKAAVDAAVAKDPASVLVLGSARLATEESWLLAQLFK
                     GTVGAPQVEFHPDLGPERRIKNPTVPGGWLVGKEAAPNSRGAEAAGVARAGKGSALAR
                     LLDGSYTPDVLHVADAELTSRASDPEFVKALRRAKTLIVHGRRTNPLTDAADVLLPVA
                     SLAGKEGTFVNVLGLVQRFQIAIVPPPIVRTDLEVLLHLGKRWGVFDTKWTAKDVFTR
                     MRDSVPGYAGLEWDEPALVGTEPPVSPVSAYDALLERRLQNPAEVLALAAEAAGAAPA
                     GGEK"
     gene            complement(3060..4427)
                     /gene="nuoF"
                     /locus_tag="P5164_09950"
     CDS             complement(3060..4427)
                     /gene="nuoF"
                     /locus_tag="P5164_09950"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRY44257.1"
                     /translation="MSEFIPVLSARVGKKNSRALETYVADGGYASWKKVLAGSQTGEW
                     TPAKVTDVVKASGLRGRGGAGFPTGMKWTFVPPKEKRGGKPVYLLCNADESEPGTFKD
                     RVLIEQDPHQILEGMMLASFALDVKHAYIYIRGEMVHGAEILNAAIDEAYAAGLLGTN
                     VLGSGLDLDITVHRGAGAYICGEETALIESLEGKRGHPRIKPPFPAVSGVWAAPTVVN
                     NVETLCCVKHILDRGADWFKGIGRNDRNTGPKLYCVSGHVNKPGVYEAPMGIPFKDLL
                     AMCGGVREGRKVKAVIPGGSSAPMLTGEEIMDVPMDFDGVAAAKSMLGSAAVIVMDDQ
                     TDIPKAVANIAKFYAHESCGQCTPCREGCPWLLKMLKRIVNGQGKKADLEMLLQVSGQ
                     MGNGMTICVFADAAIAPVLSSIPRFREEFEAYVAKSAAPGFEVPAPDQITPRLAERMA
                     EGMHS"
     gene            complement(4424..4957)
                     /gene="nuoE"
                     /locus_tag="P5164_09955"
     CDS             complement(4424..4957)
                     /gene="nuoE"
                     /locus_tag="P5164_09955"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRY44258.1"
                     /translation="MDILPGQPPVGEHGALSCGKELAFSAKAEERLQKLLTRYPTKQA
                     ALLPTLWIAQEEWGWISREAVDTVASRLGLTSAFVWGVVTFYTMYNRAPVGKHLIQVC
                     TSISCHLNGAEEVFHECRKKLGGLKPGETSADGKYTVVEVECLAQCDKAPAVMVNGDD
                     HERITLDKLDAFLAELR"
     gene            complement(4978..5340)
                     /locus_tag="P5164_09960"
     CDS             complement(4978..5340)
                     /locus_tag="P5164_09960"
                     /inference="COORDINATES: protein motif:HMM:NF012717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRY44259.1"
                     /translation="MGTPDAFLPILLMFTAAAILGAALLFLNGVMGRKVRTLRKLGPY
                     ESGLPLLDDSRKRISVKFFIVAMVFILFDVETGFLWPWATVLDEGGWVVFWPMILFLA
                     VLALADAYLWKKGAFDWE"
     gene            5350..6072
                     /locus_tag="P5164_09965"
     CDS             5350..6072
                     /locus_tag="P5164_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY44260.1"
                     /translation="MLIFTKLRGSSTTESPFRPAALVPAFRCETWVGDVVRGLLRHVP
                     EVLVVDDGSEDLTSEAAREAGAKVHRRETNGGKGRAMRDGIGLLLRGDWSHVAFVDGD
                     GQHDPDDLPTLLAAARDGADFVIGSRLSDPEGMPAKNRRANETGDKVLSRMTGLPVED
                     GQSGYRVLSTALLRRLRLTANRYAIENEMLIKAAPVLRKFAVVPVRSIYSTGHRHYRP
                     FRDTWVTSWLSVHYKTTGSDVS"
     gene            6107..6961
                     /locus_tag="P5164_09970"
     CDS             6107..6961
                     /locus_tag="P5164_09970"
                     /inference="COORDINATES: protein motif:HMM:NF023385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRY44261.1"
                     /translation="MSAARLVLTVDVEEWFHVCGHPTYDRPERWDAFPSRVVPSTERI
                     LSLLEESSSRATFFVLGWVARRHPGLVRRIAGAGHEIGCHGDLHRRATEQSVEEFRDD
                     LRAARASLEDAAGAPVTAFRAPEWSFRTTACPHLGTLVEEGFRVDSSLLAVPPIGDIG
                     NPLRPTMIPTPAGDLLEVPPLVGTFFGQPAMMGGGWTSRLSREARVSRAVEESLGRGE
                     SPVLYLHPWEVDEAHPPMELPPVARLVHFGGRSRVVPRLARLLARHRSASLAEAFPAS
                     RRSRGVAA"
     gene            6958..7944
                     /locus_tag="P5164_09975"
     CDS             6958..7944
                     /locus_tag="P5164_09975"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRY44262.1"
                     /translation="MRRFAALFPGQLSEKAGMGEALAASFPRVASFFEQVSERSGVDV
                     AGTFFGEGRPDLHADLPAQVGVFAVSVAALDVLAEEHGLYPSACAGYSLGTYAAFVGA
                     GVLDRWAALDVLLEVERLLREKAPAGTMGFVIGLPELEVAAELARLTRDPHEVAIGNV
                     NAAQQVVLTGRREPVLRALDHLAMHALKSEALPLGWPMHSPALAPVAAELAELVSSRI
                     RLAFPGRAALYAPMLGRVVRSEEEARLVITSQIARTSRWADVLLAMASAGETRFVEVG
                     PGDLLSKMHRWTLRSSRADVLEDPAGIARFASALGAPALGAPARPAAGEEVA"
     gene            7941..>8576
                     /locus_tag="P5164_09980"
     CDS             7941..>8576
                     /locus_tag="P5164_09980"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HRY44263.1"
                     /translation="MTERQRVAWVTGGSRGIGRAIAERLAVDGLHVLVTYRSDETGAA
                     ETVRRITEAGGSAEAAKADATSRDEIVAVADRLDAERGGVDVLVNNAATLRNGLFALM
                     PEAHWDEVIDTSLGGAFRSTKAVVRGMLRRRWGRIVNISSLAALMGSAGQTNYAAAKG
                     ALVAFTKSLAAEVGPYGVTVNCVAPGFVETEMISFLSKEAREDFTKRIPVRR"
BASE COUNT         1255 a         2976 c         3081 g         1264 t
ORIGIN      
        1 gacgacggag acgaggccga ggagccgctg gatcggcctc tcgtcgtgcc cttccgcttc
       61 ctgcgtgagg ttcaggagca tgacgacgaa gaggaagagg acgaggatcg cgccggcgta
      121 gacgatgacc tggatcgcgg cgatgaaggg cgccgcgagg aggacgaaga ggacggcgag
      181 cgcgaagaag gtgacgagga gcgagacgac gctcctcacg ggcgtcttca cgacgaccat
      241 cgcgagggcg gagacgatcg cgagccccga ggcgatcgcg aagacgacga gctcgagcga
      301 catcggcttc agcccctcac ttcgcggcga cgaacgccgc gacggcgatc aggtggaaga
      361 aggagagcgg caggaggaac ttccagccga ggtccatcag ctggtcgtag cggaatcggg
      421 ggaaggtcca gcggacccag aggaagagga agatgaatcc gatcgtcttc gcgaagaaga
      481 cggcgacgga ggcgagcgcg ccccagactc ccgtgaacgc gacgcccggg agcgaccagc
      541 cgccgaagta gagcgtcgcc agcagggcgg acgcggcgat caggttcgcg tactcgccca
      601 tgaagaacat gccgaacttg aacgaggagt actcggcgtg gaacccgccc gcgagctcct
      661 cctcggcctc ggcgaggtcg aaggggagcc ggttcgtctc ggcgaaggcg gcgatcagga
      721 agacgaggaa gccgacgaac tgcgggacga cgttccagac gtgctgctgc tgccactcga
      781 cgacgccgga gagccggaga gtcccggccg cgaggaagac gcccgccgcg gcgaagccca
      841 tcgcgagctc gtagctgatg atctgggccg aggcgcggag gcctcccatc agcgagtact
      901 tgttgttcgc cgaccatccg gcgaggacga tcccgtagat gccgaggccg gtgaacgcga
      961 agatgtagag gatgccgatg ttcacgtcgg cgatgacgag cggcacctcg cgcgtcacac
     1021 ctccgatcgt gacggggagc gtcgccccga acgggatgac ggcgaacgtc acgagccccg
     1081 gcatcagggt gatccagggc gcgaggttgt agaggagctt gtcgacgtaa agcggcgtca
     1141 cgctctcctt catgaagaac ttcgtgccgt ccgcgatgat ctggaagaag ccgaaggggc
     1201 cgacccggtt cggcccgggg cggtcctgga tcatcccggc gacccgccgc tccgcccaga
     1261 cgaggttcgg gaggatcgtg gcgagcgcga tgacccagac gaagaggatc ttcgccccgg
     1321 tggcgacgag ggcgaacgtg gcgtcgctca cttctcccct ccggccggag ccgctccggc
     1381 ggcttcggct gcgagcgcga ggacctcggc cgggttctgg aggcggcgct cgaggagcgc
     1441 gtcgtaggcg gagaccgggg agacgggggg ctcggtcccg acgagggcgg gctcgtccca
     1501 ctcgaggccg gcgtagccgg ggacggagtc gcgcatccgc gtgaagacgt ccttcgccgt
     1561 ccacttcgtg tcgaagacgc cccaccgctt cccgaggtgg aggaggacct cgaggtcggt
     1621 cctcacgatc ggcgggggga cgatcgcgat ctggaaccgc tggacgaggc cgaggacgtt
     1681 cacgaacgtc ccctcctttc cggcgaggct cgccaccggg aggaggacgt cggccgcgtc
     1741 ggtgagcggg ttcgtccgcc ggccgtggac gatcagcgtc ttcgcgcggc ggagcgcctt
     1801 cacgaactcg gggtcggagg cccgcgaggt gagctccgcg tcggcgacgt ggaggacgtc
     1861 cggcgtgtac gagccgtcga ggaggcgggc gagggccgag cccttcccgg cgcgggcgac
     1921 ccccgcggcc tcggcgccgc gggagttcgg cgcggcctcc tttccgacga gccacccgcc
     1981 ggggaccgtc gggttcttga tcctccgttc cgggccgagg tcggggtgga actcgacctg
     2041 gggggcgccg acggtcccct tgaagagctg cgccaggagc cagctctcct cggtcgcgag
     2101 gcgggcggag ccgaggacga ggacggaggc cgggtccttc gcgacggccg cgtcgacggc
     2161 ggccttcacc gcgtcgagcg cctcgcccca gttcgtctcg cgggcgctcc cgtccttccg
     2221 gaggacgggc cgtgtgaagt cgttcccgtt cagctcctcg gcccggaaac ggccgtagtc
     2281 gcacatccag aagtcgttca cggcgaggtt cacgcggggg aggaagcggt agacggtccg
     2341 gtcgcggtgc tcgatcgaga cgttgcagcc gatctcgcag ccgtcgcaga ccgaccgggc
     2401 cttgtcgagc caccagaccc gcttgcggaa ccggaactcc ttcgtcgtca gggcgccgac
     2461 ggggcagacg tccgccgcgc agcccgacca gtcgttcgcg agctccttcc cctcgtgggt
     2521 ccagacgatc gtgtggccgc cccgctcgat gaacgtcagc tcgccggtcc cggcgatctc
     2581 ctggcagaac cggatgcagc gcgtgcactg gatgcagcgc gtcatgtcgg cgatgacgtg
     2641 cgggccgatg ctcgtccggt cgtagccggg gaactgccgc cggggctcgg ccgtccgcga
     2701 gaccgggagg cccgccgcga cggcgttgtc ctgcagcttg cactcgcccg cctggtcgca
     2761 gatggggcag tcgagcgggt ggttgatgag gagaaactcc atcgtcgcgt tccgcgcggc
     2821 gagggccttc tccgagctcg cgaggacgac catgccgtcg cgcacgggga ccgtgcacga
     2881 ggcctggatc ttcggcaccc cctcgatctc gaccatgcac atccggcact ggccgacgac
     2941 cgagaggcgc gggtggtagc agaagtgggg gacgtcgacc ccggccgccc ggcacgcctc
     3001 gaggacgttc gtgctgtcgg ggacctcgac ggtccgtccg tcgacggtga ccttcgccat
     3061 caggagtgca tcccttccgc catccgctcg gcgagcctcg gggtgatctg gtccggtgcg
     3121 ggcacctcga agccgggcgc cgcggacttc gcgacgtacg cctcgaactc ctcgcggaag
     3181 cgcgggatcg aggagaggac gggcgcgatc gccgcatcgg cgaagacgca gatcgtcatc
     3241 ccgttcccca tctggccgga gacctgcagc agcatctcga ggtcggcctt cttcccctgg
     3301 ccgttcacga tcctcttcag catcttgagg agccaggggc acccctcgcg gcagggcgtg
     3361 cactgcccgc acgactcgtg cgcgtagaac ttcgcgatgt tcgccacggc cttcgggatg
     3421 tccgtctgat cgtccatgac gatgacggcc gccgagccga gcatcgactt cgcggcggcg
     3481 acgccgtcga agtccatcgg gacgtccatg atctcctcgc ccgtcagcat cggggccgag
     3541 gagccgcccg ggatcacggc cttcaccttc cgcccctcgc gcaccccgcc gcacatcgcg
     3601 aggaggtcct tgaaggggat ccccatcggc gcctcgtaga cgcccggctt gttgacgtgg
     3661 ccggagacgc agtagagctt cggcccggtg ttgcggtcgt tccggccgat ccccttgaac
     3721 cagtcggcgc cgcggtcgag gatgtgcttc acgcagcaga gcgtctcgac gttgttgacg
     3781 acggtcgggg cggcccagac gcccgagacg gcggggaagg ggggcttgat gcgcgggtgg
     3841 ccgcgcttcc cctcgaggct ctcgatgagg gccgtctcct cgccgcagat gtaggcgccg
     3901 gcgccgcggt ggaccgtgat gtcgaggtcg aggccgctcc cgaggacgtt cgtcccgagg
     3961 aggccggccg cgtaggcctc gtcgatcgcc gcgttcagga tctcggcgcc gtggaccatc
     4021 tccccgcgga tgtagatgta ggcgtgcttc acgtcgagcg cgaagctcgc gagcatcatc
     4081 ccctcgagga tctggtgggg gtcctgctcg atgaggaccc ggtccttgaa cgtcccgggc
     4141 tccgactcgt cggcgttgca gaggaggtag acgggcttgc cgccccgctt ctccttcggc
     4201 gggacgaagg tccacttcat gccggtgggg aagccggcgc cgccccggcc ccggaggccc
     4261 gaggccttca cgacgtcggt caccttcgcc ggcgtccact cgcccgtctg cgacccggca
     4321 aggaccttct tccaggaggc gtagccgccg tcggcgacgt aggtttcgag ggcgcgggag
     4381 ttcttcttcc cgacgcgggc ggagaggacg ggaatgaatt cgctcatctc agctcggcga
     4441 ggaaagcgtc cagcttgtcg agggtgatcc gttcgtggtc gtcgccgttc accatgacgg
     4501 cgggcgcctt gtcgcactgc gcgaggcact ccacctcgac gacggtgtac ttcccgtcgg
     4561 ccgaggtctc gcccggcttc aggccgccga gcttcttccg gcactcgtgg aagacctcct
     4621 ccgcgccgtt caggtggcag gagatcgacg tgcagacctg gatgaggtgc ttcccgacgg
     4681 gggcccggtt gtacatcgtg tagaacgtca cgacgcccca gacgaaggcc gaggtgaggc
     4741 cgagccgcga ggcgacggtg tcgaccgcct cgcgcgagat ccagccccac tcctcctgcg
     4801 cgatccagag cgtcgggagg agggcggcct gcttcgtcgg gtaccgggtc aggagcttct
     4861 ggagccgctc ctcggccttc gccgagaacg cgagctcctt gccgcaggag agggccccgt
     4921 gctcgcccac ggggggctgg ccggggagga tgtccacggg ggtgctcggg ccgccgctca
     4981 ttcccagtcg aacgctccct tcttccagag gtaggcgtcc gcgagggcca ggacggcgag
     5041 gaacaggatc atcggccaga agacgaccca gccgccctcg tcgaggacgg tggcccacgg
     5101 ccagaggaat cccgtctcca cgtcgaagag gatgaagacc atcgcgacga tgaagaactt
     5161 gacggatatc cgctttcggc tgtcatccag gagcggcagg ccgctctcgt acgggccgag
     5221 cttgcggagg gtccggacct tgcggcccat caccccgttc aggaacagga gggcggcccc
     5281 gaggatcgcg gccgccgtga acatgaggag gatggggagg aaggcgtcag gtgtgcccat
     5341 gaggcgctcg tgctaatttt cacaaagttg cggggatcgt caaccacgga aagcccgttc
     5401 cggcccgccg cgctcgtccc cgcgtttcgg tgcgaaacgt gggtcggcga cgtggtacga
     5461 ggcctcctcc ggcacgtgcc ggaggtgctc gtcgtcgacg acgggagcga ggatctcacg
     5521 tccgaggccg cccgggaggc cggagcgaaa gttcaccgcc gcgagacgaa cggtggtaag
     5581 gggcgggcga tgcgcgacgg catcggtctc ctcctccggg gcgactggtc ccacgtggcg
     5641 ttcgtcgacg gggacggcca gcacgacccc gacgaccttc cgacgctcct cgccgccgcg
     5701 cgggacggag ccgacttcgt gatcggctcg cgcctctccg acccggaagg gatgccggcg
     5761 aagaaccgcc gcgcgaacga gacgggcgac aaggtcctct cgcggatgac cggcctgccg
     5821 gtcgaggacg ggcagtcggg ctaccgggtc ctctcgaccg cgctcctgcg gcggctccgc
     5881 ctcaccgcga accgctacgc catcgagaac gagatgctca tcaaggccgc tccggtcctc
     5941 cggaagttcg cggtcgtgcc ggtccgttcg atctactcga ccggccacag gcactaccgg
     6001 ccgttccggg acacctgggt cacttcctgg ctctcggtcc actacaagac gaccggctcc
     6061 gacgtctcct gatgcgcctc gcgcccataa tccataatcc gccgccatgt ccgcagcccg
     6121 gctcgtcctg accgtcgacg tcgaagagtg gttccacgtc tgtggccacc cgacgtacga
     6181 ccgcccggag cgatgggacg cgttcccgtc ccgcgtcgtc ccgtccacgg agcggatcct
     6241 ctccctgctc gaggagagct cctcgcgggc gaccttcttc gtcctcggct gggtggcgcg
     6301 aaggcacccg gggctggtcc ggcggatcgc cggcgcgggg cacgagatcg gttgccatgg
     6361 agacctgcac cgccgcgcga ccgagcagag cgtcgaagag ttccgggacg acctgcgcgc
     6421 ggcccgcgcg tcgctcgagg acgccgcggg agcgcccgtc acggccttcc gcgcccccga
     6481 gtggtcgttc cggacgacgg cctgcccgca cctcggaacg ctcgtcgagg agggcttccg
     6541 ggtcgattcg tcgctccttg ccgtcccgcc gatcggagac atcggaaatc cccttcgacc
     6601 gacgatgatt ccgaccccgg cgggcgacct gctggaggtc cccccgctcg tcggcacctt
     6661 cttcgggcag ccggcgatga tgggcggagg ctggacctcc cggctctcgc gggaggcccg
     6721 ggtctcgcgg gccgtcgagg agtctctcgg ccgaggggag agccccgtcc tctacctcca
     6781 cccctgggag gtcgacgagg cccaccctcc catggagctg ccccccgtcg cccgcctggt
     6841 ccacttcgga ggccggagcc gggtcgttcc gaggctcgcc cggctcctcg cccgccaccg
     6901 gtcggcctcg ctcgccgagg cgttcccggc ctcccgccgg agccgggggg tggcggcgtg
     6961 aggcggttcg cggctctctt cccggggcag ctctccgaga aggcggggat gggcgaggcg
     7021 ctcgccgcct cgttcccgcg cgtcgcctcc ttcttcgagc aggtttcgga acggagcggc
     7081 gtcgacgtcg cggggacctt cttcggcgag ggacgccccg acctccacgc cgacctcccc
     7141 gcgcaggtcg gcgtcttcgc cgtctcggtc gccgcgctcg acgtcctcgc cgaggagcac
     7201 ggcctctacc cctcggcctg cgccggctac agcctcggga cctacgccgc tttcgtcggc
     7261 gcgggcgtcc tcgaccgctg ggccgcgctc gacgtcctcc tggaggtcga gcgcctgctc
     7321 cgcgagaagg cgcccgccgg gacgatgggg ttcgtcatcg gcctcccgga gctggaggtc
     7381 gcggccgagc tcgcgcgcct gacgcgggac ccgcacgagg tggcgatcgg caacgtgaac
     7441 gcggcccagc aggtcgtgct gacgggtcgg cgcgagccgg tgctgcgcgc gctcgaccac
     7501 ctcgcgatgc acgccctgaa gtccgaggcg cttccgctcg gctggccgat gcactctccg
     7561 gctctcgcgc cggtggccgc ggagctcgca gagctcgtct cgagccggat ccgtctcgcc
     7621 ttcccaggcc gcgccgcgct ctacgccccg atgctcgggc gcgtcgtacg gagcgaggag
     7681 gaggcccgcc tcgtcatcac gagccagatc gcccgcacgt cccgctgggc cgacgtcctc
     7741 ctcgcgatgg cctcggccgg ggagacccgg ttcgtcgagg tcggcccggg ggacctcctc
     7801 tccaagatgc accgctggac gctccggagc tcccgcgcgg acgtcctcga ggacccggcc
     7861 gggatcgccc ggttcgcctc ggctctcggc gcgccggccc tcggcgcccc cgctcggccc
     7921 gcggccggcg aggaggtcgc gtgacggaga ggcagagggt cgcctgggtg accggcggct
     7981 cgcgcggcat cgggcgggcg atcgccgagc gcctcgcggt cgacgggctc cacgtcctcg
     8041 tcacctatcg ctccgacgag accggggcgg ccgagacggt ccgccggatc accgaggcgg
     8101 ggggcagcgc cgaggccgcg aaggccgacg cgacctcccg ggacgagatc gtcgccgtcg
     8161 ccgaccgcct cgacgcggag cgcggggggg tcgacgtcct cgtgaacaac gcggcgaccc
     8221 tccgcaacgg cctcttcgcg ctcatgccgg aggcgcactg ggacgaggtg atcgacacct
     8281 cgctcggagg ggcgttccgt tcgacgaaag ccgtcgtccg gggaatgctc cggcgccgct
     8341 gggggcgaat cgtcaacatc tcttcgctcg cggccctcat gggctcggcc gggcagacga
     8401 actacgccgc ggcgaagggg gcgctcgtcg ccttcacgaa gtcgctcgcc gcggaggtcg
     8461 gcccctacgg cgtcaccgtc aactgcgtgg cgcccggctt cgtggagacc gagatgatct
     8521 cgttcctctc gaaggaagcc cgcgaggact tcacgaagcg gatccccgtc cggcgc
//
LOCUS       DAOTEZ010000164         8555 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5855_length_8555_cov_6.106000, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000164 DAOTEZ010000000
VERSION     DAOTEZ010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8555
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5855_length_8555_cov_6.106000"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..891
                     /locus_tag="P5164_09985"
     CDS             178..891
                     /locus_tag="P5164_09985"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HRY44264.1"
                     /translation="MSEITDRIEVSRETPAYPARHDVRDGHLVVGGERVPVVVKRIPR
                     DFRQPEGKTRAERSLRIARHLLAHGVDTPEPLGIEVTPEESVLVVRKVEGAVEVRAFF
                     LRRNAPSLGALPLRASFEEVLTALARFARRMHDSGVFFRDFSDGNVLVTDGESGPRLW
                     IVDLDRARIRRPPLATFTRLRDLSRPGLTRAEDRRRLLRAYWAPDPVPAMAPALHAAL
                     RFRLLAWNRLKAALRPWRR"
     gene            1015..1638
                     /locus_tag="P5164_09990"
     CDS             1015..1638
                     /locus_tag="P5164_09990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRY44265.1"
                     /translation="MANDLPGEGTPAQEPGIRERERELLERVRGGEPDAFEHFVRTYQ
                     RKVFRLVYTLVRNAADADALTQDVFVKAWRAVPDFKGDAAFETWLNRIAVNAVRDAAR
                     RRRPVVSFTDLADADETGSSRLPPAIEPVDATTPEREARSRQIRRRIGEALEALSPRQ
                     KAIFVMKYYEDRTVAEIGAAIGVDDGTVKSHLFRAARKLRQKLEDLR"
     gene            1635..2171
                     /locus_tag="P5164_09995"
     CDS             1635..2171
                     /locus_tag="P5164_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44266.1"
                     /translation="MSGRHLTEDEVVNRVFPAQDGTAPIPIHQAVCPECQERVARLRE
                     AFLLDRGALTGVVESFPPEFWTAQRKAVRGRISELAALEAEERATPFPARLTRSVLQR
                     PALAFGSLAAALALVAGLTVLRGGGEPNVEPVQTVASAEEAAVFSAADTADDELLRAV
                     DVALTTESPYETVLPEAM"
     gene            2171..2731
                     /locus_tag="P5164_10000"
     CDS             2171..2731
                     /locus_tag="P5164_10000"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HRY44267.1"
                     /translation="MKRALVLSTLLGAALAALPAPAQLLEMPLGKWWKRPAVVEALKL
                     APEQQQRLEEVFSKNRRTFVDLKADVERRTIDLEDLLAAREVEPRKVAAASEALEQAR
                     ARLGKARTMMVVEMRGVLTGDQWATIVDRREEFRAERRAEVRRRFADRRNGGRPGHPT
                     ARPGTPAPSAPPDPDEGSDVPDEPEP"
     gene            complement(2728..3525)
                     /locus_tag="P5164_10005"
     CDS             complement(2728..3525)
                     /locus_tag="P5164_10005"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY44268.1"
                     /translation="MTTARRPPYAVLAGYYDEVYGLWRDLLGPARDEILRAHRVRFET
                     LLDVGCGSGWQSLELARRGARVVAVDPAHPFLAPLRAIARRERLPIDVRHGDVLSLPL
                     EEGETFDLALATFDVLNHLDRHEELGPALAGVAKALRPGGHLLFDLNTPETIEAFPDH
                     HRVTHLSGGGLSAERGAFDPESGAGTVTRDWFLPVTGAPGRFRRVHELYREIAWTAAA
                     VRRALREAGLEVRLFADASGWLSFQPEETRWIVLARRRSAGRRAGGS"
     gene            complement(3522..4382)
                     /locus_tag="P5164_10010"
     CDS             complement(3522..4382)
                     /locus_tag="P5164_10010"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY44269.1"
                     /translation="MTGFAEIAFLLAAPLLLAATGELLLERSGSIQIGLEGTMLFGAW
                     VAFAAGRAGLPAWGGLLSAALAGLGAGLLFALFAVVRRADPILVGTVWNLLALGATAF
                     AYRLGAGETGSVLEVATLPRGLFGLPDAVAASFLAPVVLHLWLTRTRAGLRLSAAGEN
                     PEALRSLGISVVAVRSGAAAASGVLGAMGGAVLVLTVSPTFVEGVTAGRGFLALALVV
                     FARWKPLALLPSALLLGAVTALQYRLQAEGFGLPYALFLALPGLVALAALALASTRGG
                     APKALGSPAP"
     gene            complement(4379..5374)
                     /locus_tag="P5164_10015"
     CDS             complement(4379..5374)
                     /locus_tag="P5164_10015"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY44270.1"
                     /translation="MRPAAVAAVAAGTLAAAALLVLAAGASPADAFVALLRGAVGSSA
                     ALGETLARSTGLVLTALGAVVAFRAGVLNIGLEGQFLAGAAAAAAAGPLFPDAPLAAR
                     AAVLLAAPLAGALQAAPAAWLAERRQVPAVLSTILLNLVAAAGVTWLVRGPLRDPSGD
                     YPQSRALADAALLPPLAGGSRATAAILLALLLAAAGAFLLARTPLGLALRAVGEAPRA
                     ARAAGLPDVGLRVGAFLSSGALAGLAGGLEVLAVTGRLYDPFTAGVGYTGITAALLGG
                     MSTIGGAASALLFAALGAGSSALQRDAGVPSSLASIVPALAVLALLLARGGRRER"
     gene            complement(5371..6870)
                     /locus_tag="P5164_10020"
     CDS             complement(5371..6870)
                     /locus_tag="P5164_10020"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY44271.1"
                     /translation="MSDPGAALELNGIRKAFGPVTALDGARLTIRPGELHALLGENGA
                     GKSTLVAIAAGLLRADAGEVRRDGRLLPAADARAARRAGIALLPQHDLLVEAATVADN
                     LALLDPAAPLIESAGARRARVLRLEGRLGLELGPPGARVGELPVGVRQRIAVAAVLQS
                     DPGVLVLDEPTAVLSPQETSALFRSLRARVATGRAVLLITHRLAEVFEGANRLTLLSR
                     GRTLLEAEVSSTTPEAVGALLLGGAEVSSRSRALRPEPSDGERLPALAVEDLLPAGAT
                     GPPAFFSVPAGTALTLLAVDGNGADLLGSAIAGVRPARGRIRLAGRPVPPGDPRAFRA
                     AGGAFIPADRRAEGLVPGLSLAENLFLRRPPGRFVIDRAALAAAADERLRRFGVRARG
                     PGAEAGELSGGNQQKLLLARELEPLPRLLVAVHPTRGLDLASSSDVRARLLDAARAGA
                     GVLVATADPEEALELGAPIRVVYRGALSPPLAATTTLAELGLRMAGLEP"
     gene            complement(6902..7894)
                     /locus_tag="P5164_10025"
     CDS             complement(6902..7894)
                     /locus_tag="P5164_10025"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HRY44272.1"
                     /translation="MTPRRRFLASLLALPLLGSLPSCGRPPAAKPAEGFRVALLTPGP
                     VSDAGWNAGAFEGLKAIERELGAKVSHVQVKTPSEFEEQFRAYAAGGAALCIGHGFEF
                     QDAAARVAKDYPRTVFLTTSGSRTSRNQAPLVFELEQATYLCGLVAGRMSRTAKAGMV
                     GGVEIPSIKSTFLAFEAGLKAGNPGAKATAVFTGSFEDVAGAKTAALALIDGGSDFLF
                     HNANAAALGVFAAATERGVYAFGANRDQNAVSPSVLASAVIDLPRAFVEAARRVKEGR
                     FDGQPIRYGLASGVVSFVWNPALLGRVPAPVVDEVDRAKEKIVSGAIIVPRGNF"
     gene            complement(7914..>8555)
                     /locus_tag="P5164_10030"
     CDS             complement(7914..>8555)
                     /locus_tag="P5164_10030"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY44273.1"
                     /translation="HDVAVHFRSDPEAAARTVAEITRLGARAEAFRADLRAPGAPQGL
                     VGSVLGRFGRLDLLLHSASPWLEKPVDEVTEEGWDDVFSVGPRAGFFLARAAASALAA
                     SGGAVLLVSDVAATKAWPRHLPHAVSKAAVDALVRNLAVALGPSVRVNGIAPGIVLPP
                     EELPPEEVARLVARTPLGRAVSVDDLVSAALFLASNRSITGQVLAVDAGRSAV"
BASE COUNT         1211 a         3035 c         3397 g          912 t
ORIGIN      
        1 aggcgtcggt cgcctggatg acgtcgtcgc tctcgaacgg catcaggaac tccctcgggt
       61 cggcgagccc gtcgacgacg agctggaagg tgtgaaggtg ctttcgatcc atagtctcct
      121 cgggacctcc gcgttccggc ccggtggacc ggcgccggca tcgtatcctc tgcccgtttg
      181 agcgagatca ccgacaggat cgaggtgagc cgggagacgc cggcctaccc ggcccggcac
      241 gacgtgaggg acggtcacct cgtcgtgggg ggcgagcggg tcccggtcgt cgtgaagagg
      301 atcccgcgcg actttcgtca gcccgaagga aagactcgcg ccgagcgctc ccttcggatc
      361 gcccgccacc tcctcgccca cggcgtcgac acgccggagc cgctcgggat cgaggtgacg
      421 cccgaggaga gcgtcctcgt cgtccggaag gtcgaggggg ccgtcgaggt gcgggcgttc
      481 ttcctgcgac gcaacgcacc gtcgctcggg gcgcttccgc tccgcgccag cttcgaggag
      541 gtcctgaccg ccctcgcccg cttcgctcgc cggatgcacg attcgggggt cttcttccgc
      601 gatttctccg acgggaacgt cctcgtcacg gacggggagt cggggccccg cctctggatc
      661 gtcgatctcg accgcgcccg gatccggagg ccgccgctcg cgaccttcac ccggctccgg
      721 gacctgtccc ggccggggct gacccgtgcg gaagaccggc gacgccttct gagggcctac
      781 tgggcgccgg acccggtccc ggccatggcc ccggccctcc acgcggccct ccgcttccgg
      841 ctcctcgcct ggaaccgcct gaaagccgcc ctcagaccct ggaggcgctg aggagctatc
      901 ctcgccggag taaacccccg tgccgccgga cgcgtccttc ccgtcatggg gacggcctcg
      961 acgacggccc ggatcctgat gctcgaggaa ggtcctcgag ggaggagcac ggacgtggcg
     1021 aacgacctcc cgggagaggg gacgcccgcg caggaacccg ggatccggga acgcgagcgg
     1081 gagctcctcg agcgggtgcg aggcggcgag cccgacgcct tcgagcattt cgtgcggacg
     1141 taccagcgga aggtcttccg gctcgtctac acgctcgttc ggaatgcagc ggacgccgac
     1201 gccctcaccc aggacgtctt cgtgaaggcc tggcgcgccg tcccggactt caagggggac
     1261 gcggcgttcg agacgtggct gaaccggatc gccgtcaacg ccgtccggga cgccgcgcgc
     1321 cggcgccggc cggtcgtctc gttcaccgac ctcgcggacg ccgacgagac cggctcctcg
     1381 cgccttccgc cggcgatcga gccggtcgac gccacgacgc cggaacggga ggcccgctcg
     1441 cggcagatcc gccggaggat cggcgaggcg ctcgaagccc tgtcgccccg gcagaaggcg
     1501 atcttcgtga tgaagtacta cgaggaccgg acggtcgccg agatcggcgc cgccatcggc
     1561 gtcgacgacg gaacggtcaa gtcgcacctc ttccgcgccg cgcggaagct gcgccagaag
     1621 ctggaggacc ttcggtgagc ggacggcatc tgacggagga cgaggtcgtg aaccgggtct
     1681 tcccggccca ggacggcacg gcgccgatcc cgatccacca ggcggtctgc cccgagtgcc
     1741 aggagcgggt cgcccgtctc cgcgaggcgt tcctcctgga tcgcggcgcg ctgacggggg
     1801 tcgtcgagtc gttcccgccg gagttctgga cggcccagag gaaggccgtg agggggcgca
     1861 tctccgagct cgcggcgctc gaggccgagg agagggcgac gccgttcccg gcgcgcctga
     1921 cccgctccgt cctccagagg ccggccctcg ccttcggcag cctcgccgcc gccctcgccc
     1981 tcgtcgccgg cctcacggtc ctgcgcgggg gcggagagcc gaacgtggag ccggtgcaga
     2041 ccgtggcgtc ggccgaggaa gccgccgtgt tctccgcggc cgacaccgcc gacgacgagc
     2101 tccttcgcgc ggtggacgtg gctctgacga ccgagtcgcc ctacgaaacg gtcctcccgg
     2161 aggcgatgtg atgaagcgcg ccctcgtcct ttccacgctt ctcggcgccg cgctcgcggc
     2221 gcttcccgcc ccggcccagc tgctcgagat gccgctgggc aaatggtgga agcgccccgc
     2281 ggtcgtcgag gcgctgaagc tcgcgcccga gcagcagcag cgcctcgagg aggtcttctc
     2341 gaagaaccgc cggacgttcg tcgacctgaa ggccgacgtc gagcggcgga cgatcgacct
     2401 cgaggacctc ctcgccgccc gggaggtcga gccgcggaag gtggccgcgg cctccgaagc
     2461 gctcgagcag gctcgggcgc ggctcggcaa ggcgcggacg atgatggtcg tggagatgcg
     2521 gggggtcctg acgggcgacc agtgggccac gatcgtcgac cgccgcgagg agttccgggc
     2581 cgaacgccgg gccgaggtcc gccgccggtt cgcggaccgg cggaacggcg ggaggcccgg
     2641 gcaccccacg gcgcgacccg ggaccccggc tccctcggcg ccaccggacc cggacgaggg
     2701 gtcggacgtg ccggacgagc cggagcccta ggagcctccc gccctccgtc cggcgctgcg
     2761 ccggcgggcc aggacgatcc agcgggtctc ctcgggctgg aatgagagcc agccggaggc
     2821 gtccgcgaag agccggacct cgaggcctgc ctcgcgaagg gctcgcctca cggcggcggc
     2881 ggtccaggcg atctcgcggt agagctcgtg gactcggcgg aagcggccgg gcgcgcccgt
     2941 cacggggagg aaccagtcgc gggtgacggt gccggcgccg ctctccgggt cgaacgctcc
     3001 gcgctcggcc gagagcccgc cccccgacag gtgcgtgacc ctgtggtggt cggggaacgc
     3061 ctcgatcgtc tccggggtgt tcaggtcgaa gagaaggtgc ccgcccggac gaagggcctt
     3121 cgcgacgccc gcgagggcgg gaccgagctc ctcatggcgg tcgaggtggt tcaggacgtc
     3181 gaacgtggcg agggcgaggt cgaacgtctc gccctcctcg agcgggaggg aaaggacgtc
     3241 gccgtgccgc acgtcgatcg ggagccgctc gcgccgcgcg atcgcgcgca ggggcgcgag
     3301 gaacgggtgc gccgggtcga cggcgacgac gcgcgcgccg cggcgggcca gctcgaggct
     3361 ctgccacccg ctgccgcagc cgacgtccag gagcgtctcg aaccggacgc ggtgcgcccg
     3421 gaggatctcg tcgcgcgcgg ggccgaggag gtcgcgccag agaccgtaga cctcgtcgta
     3481 gtagccggcg aggacggcgt agggaggacg gcgggccgtc gtcacggcgc ggggctcccg
     3541 agggccttcg gcgccccgcc gcgggtggat gcgagggcga gagccgcgag cgcgacgagg
     3601 ccgggcagcg cgaggaagag cgcgtagggg aggccgaagc cctccgcctg gagacggtac
     3661 tggagggccg tcacggctcc gaggaggaga gccgatggaa gcagcgcgag cggcttccat
     3721 cgcgcgaaga cgacgagcgc gagcgcgaga aagccgcggc cggcggtgac gccctcgacg
     3781 aaagtgggcg agacggtcag gacgagcacg gcacctccca tcgcgccgag gacgccggac
     3841 gcggcggcgg cgccgctccg gaccgcgacg accgagatcc cgagcgagcg gagcgcctcc
     3901 ggattctctc ccgcggccga gagccggagg ccggcgcgcg tccgggtgag ccagagatga
     3961 aggacgaccg gcgcgaggaa gctcgcggcg acggcgtcgg gaaggccgaa gagaccccgg
     4021 ggaagcgtcg cgacctcgag gaccgagccg gtctctccgg cgccgagacg gtaggcgaac
     4081 gccgtcgcgc cgagggcgag gaggttccag acggtcccga cgaggatcgg atcggcccgc
     4141 cgcacgacgg cgaagagcgc gaacaggagc ccggcgccga gcccggcgag ggccgccgag
     4201 aggaggccgc cccaggccgg caggccggcg cgccctgcgg cgaaggcgac ccacgccccg
     4261 aacagcatcg ttccctcgag gccgatctgg atcgagccgg agcgctcgag gaggagctcg
     4321 cccgtcgcgg cgaggaggag cggggccgcg agcaggaacg cgatctcggc gaagcccgtc
     4381 atctctccct ccgtcctccc cgggcgagga ggagagcgag caccgcgagc gccgggacga
     4441 tcgacgccag cgaggacggg acgccggcgt cgcgctgcag cgcgctcgag ccggcgccga
     4501 gagcggcgaa gaggagcgcc gacgcggccc cgccgatcgt cgacatgccg ccgaggagag
     4561 ccgcggtgat cccggtgtag ccgaccccgg ccgtgaacgg gtcgtagagc cgcccggtca
     4621 cggcgagcac ctcgaggccc ccggcgaggc ccgcgagggc gccggacgag aggaacgccc
     4681 cgacgcgcag cccgacgtcc ggcaggccgg cggcgcgggc ggcgcggggc gcctctccga
     4741 ccgcgcggag cgcgaggccg agcggcgtcc gcgcgaggag gaaggcgccg gcggccgcga
     4801 ggaggagggc gaggaggatc gccgccgtcg cccgcgaccc gccggccagc ggcggcagga
     4861 gggcggcgtc cgcgagcgcg cggctctgcg gatagtcgcc cgaggggtcg cggagcgggc
     4921 cgcgcacgag ccacgtcacc ccggccgccg cgacgaggtt gaggagaatc gtcgagagga
     4981 cggccgggac ctgccgccgc tcggccagcc aggcggccgg ggcggcctgc agggccccgg
     5041 cgagcggggc cgcgaggagg acggccgcgc gggctgcgag gggcgcgtcg gggaagagcg
     5101 gtcccgcggc ggcggccgcg gcggcgcccg cgaggaactg tccttcgagg ccgatgttca
     5161 ggaccccggc gcggaacgcg acgacggcgc cgagcgccgt caggacgagc ccggtcgagc
     5221 gcgcgagcgt ctcgccgagc gcggccgaag agccgaccgc tccccggaga agcgcgacga
     5281 acgcgtcggc cggcgaggcg ccggcggcga ggacgaggag ggccgcggcg gcgagcgtcc
     5341 cggcggcgac ggcggcgacg gcggccggcc tcacggctcg agccccgcca tcctgaggcc
     5401 gagctcggcg agcgtcgtcg tcgcggccag cgggggggag agcgcgccgc gatagacgac
     5461 ccggatcggc gccccgagct cgagcgcctc ctcgggatcg gccgtcgcca cgaggacgcc
     5521 cgcccccgcg cgggccgcgt cgaggagccg ggccctcacg tccgaggagg aggcgaggtc
     5581 gagcccgcgc gtcgggtgga cggcgacgag gagacggggc agcggctcga gctcccgcgc
     5641 gaggaggagc ttctgctggt tgccgcccga cagctcgccg gcctccgccc cggggccgcg
     5701 ggcccggacc ccgaagcggc gaagccgctc gtcggcggcg gcggcgagcg cggcgcggtc
     5761 gatcacgaaa cgtcccggcg gccgtctgag gaagaggttc tcggcgagcg agagccccgg
     5821 gacgaggcct tcggcccggc ggtcggccgg gatgaacgcg ccgccggccg cgcgaaaggc
     5881 gcgcgggtcg cccggcggaa cgggccgccc ggcgaggcgg atccgtccgc gggcgggccg
     5941 cacgccggcg atcgcggagc cgagcaggtc ggcgccgttc ccgtccaccg cgaggagcgt
     6001 gagggcggtg ccggcgggga cggagaagaa cgcgggcggg ccggtcgccc cggcggggag
     6061 gaggtcctcg acggcgagcg cgggcaaccg ctctccgtcc gatggctcgg gacggagcgc
     6121 gcgggagcga gacgagacct cggctccgcc gaggaggagc gctccgactg cttcgggcgt
     6181 cgtcgaggag acctccgcct cgaggagggt cctcccgcgg gagaggagcg tcaggcggtt
     6241 cgcgccctcg aagacctcgg ccagccggtg cgtgatgagg aggacggcgc ggccggtggc
     6301 gacccgcgcc cgcagcgatc ggaagagggc gctcgtctcc tggggcgaga ggacggcggt
     6361 cggctcgtcg aggacgagca cccccggatc ggactggagg actgcggcca ccgcgatccg
     6421 ctggcggacc ccgaccggaa gctctccgac gcgcgcaccg ggcggcccaa gctcgagccc
     6481 gagccgcccc tcgagccgga ggacgcgcgc gcgacgcgca cccgccgatt cgatgagcgg
     6541 cgcggcgggg tcgaggagcg cgaggttgtc ggcgaccgtc gcggcctcga cgaggaggtc
     6601 gtgctgcggc aggagggcga tgccggcgcg tcgcgcggcg cgggcgtccg ccgcgggaag
     6661 gagcctgccg tcccgccgga cctcgcccgc gtcggcgcgg aggagtccgg cggcgatcgc
     6721 gaccagggtc gacttcccgg ccccgttctc gccgaggagc gcgtggagct cgccggggcg
     6781 gatcgtgagg cgggctccgt cgagggccgt cacggggccg aacgctttcc ggatcccgtt
     6841 cagctcgagc gcggcgccgg ggtcgctcat cccgtcgtcg ccggcctcac cggggcgacc
     6901 gtcagaagtt ccctcgcggg acgatgatcg ccccggagac gatcttctcc ttcgctcggt
     6961 ccacctcgtc gacgacgggg gccgggacgc ggccgaggag cgccgggttc cagacgaacg
     7021 agacgacgcc gctcgcgagg ccgtagcgga tcggctggcc gtcgaagcgc ccttccttca
     7081 cgcggcgcgc cgcctcgacg aaggcgcggg ggaggtcgat gacggccgac gccaggacgc
     7141 tgggcgacac ggcgttctgg tcgcggttcg cgccgaaggc gtagacgccc cgctcggtcg
     7201 ccgcggcgaa gacgccgagc gcggcggcgt tcgcgttgtg gaagaggaag tcgctgccgc
     7261 cgtcgatgag ggcgagcgcg gccgtcttgg cgccggccac gtcctcgaag gagccggtga
     7321 agaccgccgt cgccttcgcg cccgggttcc cggccttcag gcccgcctcg aacgcgagga
     7381 acgtcgactt gatcgacggg atctcgacgc cgccgaccat cccggccttc gccgtccgcg
     7441 acatccggcc ggccacgagg ccgcagaggt acgtcgcctg ctcgagctcg aagacgagcg
     7501 gggcctggtt gcgcgacgtc cgcgagccgg aggtcgtcag gaagacggtg cgcggatagt
     7561 ccttcgccac gcgggccgcg gcgtcctgga actcgaagcc gtgcccgatg cagagcgcgg
     7621 cgcccccggc ggcgtaggcc cggaactgct cctcgaactc ggacggcgtc ttcacctgga
     7681 cgtgggagac cttcgcgccg agctcccgct cgatcgcctt cagcccctcg aaggcgccgg
     7741 cgttccaccc ggcgtcggag acggggccgg gagtcaggag cgccacccgg aagccttcgg
     7801 ccggcttcgc ggcgggaggc cggccgcagg atgggaggga gccgaggagc ggcagcgcca
     7861 ggagcgaggc gaggaatcgg cggcgcggcg tcatcggcgc gagtttagtc cgctcagacg
     7921 gcggaccggc ccgcgtcgac cgccagcacc tgcccggtga tcgagcggtt cgatgcgagg
     7981 aacagcgcgg ccgagacgag gtcgtcgacg gaaacggcgc ggccgagggg cgtccgcgcg
     8041 acgaggcggg cgacctcctc gggcggcagc tcctccggcg gaagaacgat gcccggcgcg
     8101 atcccgttca ccctcaccga ggggccgagg gccacggcga ggtttcgcac gagcgcgtcg
     8161 accgccgcct tggagaccgc gtgggggagg tgccgcggcc aggccttcgt cgcggccacg
     8221 tcggagacga ggaggacggc gccgccggag gcggccaggg cggacgcggc cgcccgggcg
     8281 aggaagaagc cggcgcgagg cccgacggag aagacgtcgt cccagccctc ctccgtcacc
     8341 tcgtcgaccg gcttctcgag ccacggcgag gccgagtgaa ggagcaggtc gagccggccg
     8401 aagcgcccga ggaccgaccc gacgaggccc tgaggcgcgc ccggagcgcg gaggtcggcc
     8461 cggaacgcct cggcgcgggc gccgaggcgg gtgatctcgg cgacggttcg agccgccgcc
     8521 tccgggtcgg agcggaagtg gacggccacg tcgtg
//
LOCUS       DAOTEZ010000165         8516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5885_length_8516_cov_7.069259, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000165 DAOTEZ010000000
VERSION     DAOTEZ010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8516
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5885_length_8516_cov_7.069259"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="P5164_10035"
     CDS             <1..1281
                     /locus_tag="P5164_10035"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY44274.1"
                     /translation="LARVAPRVVAILEDNFNFLTKMCTQRSREASVRLIRSAKAAGAR
                     VAVNGSDASDDPAFYLDVGADAVILGETERTIVELLDAWDRSPDADLSEIAGLALPAG
                     AVRAAGSAPTRTPPRPFVADLDALPLPAWDLVDVERYRKAWTEAHGRLSWNMVTTRGC
                     PYRCNWCAKPLYGVRYAQRSPELVAAEMKLLKETVGPDHVWFADDIFGLSHRWTERFA
                     EEVRELDASIPFTIQSRVDLMSPTAVEALRRAGAEEVWMGVESGAQSILDAMDKGTTI
                     AQVRGATRRLKEAGIRASWFLQLGYPGETWAEILATRELVREERPSDVGVSVAYPLPG
                     TVFYERVKSQLGERSHWKDSDDLDMMFEGTYAAPFYKRVRDLLHEEAALGAISPSETI
                     GRRRRNLDAAWLAAERDEPRSRTGGAALPTRQRG"
     gene            1305..2693
                     /locus_tag="P5164_10040"
     CDS             1305..2693
                     /locus_tag="P5164_10040"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY44275.1"
                     /translation="MTDLLLSHGYFLAEDEKERAVMKPYPPLGLLSLSAYLKREGFSV
                     EVFDSTFATREALCARLDEGPGVLGLYTNLMTRPPVLSITAAAKARGWTVVLGGPESA
                     NYPAEYLAAGADVVVRGEGEETLRELLPALAARGPHQLDGIAGVAFRDEEGAVVSGPP
                     RAQVRSLDTLPWPDREAIDHGPYLAAWRERHGEGSVNLITARGCAYKCDWCSHAVFGN
                     THRRRSPANVAAEVEWIRDRYRPEQVWYADDVFTISHRWLFEYAGELEKRGLKFPFET
                     ISRADRMADEEVLATLARLGCRRIWIGSESGSQRLLDAMRRGVTVEQVRWATHAARRH
                     GIEVGMFLMWGYDGETAEDVEATVEHVRASLPDVYFTTLAYPIMGTGYYEKVKGRVSL
                     SRPWAEGGDRDHVVAGRAGRDTYRLADRWLRAAVESRRLEERAPEEARALAGEAEAAR
                     REFLSAFAEGAA"
     gene            2690..3469
                     /locus_tag="P5164_10045"
     CDS             2690..3469
                     /locus_tag="P5164_10045"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRY44276.1"
                     /translation="MIAAASPFDALAATYDEDFTRTDLGRRLRSAVWRHLDATFAPGA
                     RVLELGCGTGEDALHLASRGVRVVATDASGEMVAETVRKAGAANLSHLVEARRVPFEE
                     LDAAGPSLGTFDGAFSNFGALNCAAALPPVAEALGRLLPSGAPLLLVVMGRWVPWEWA
                     FYLRRGDGARAFRRLRRDGTVWRGLTVRYPSPGRLLRTFSPWFLRRRLAALGVFLPPS
                     YAASSVSPSTLARLDRWERRIESAPLLPRLADHTLFVLERR"
     gene            3466..4533
                     /locus_tag="P5164_10050"
     CDS             3466..4533
                     /locus_tag="P5164_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY44277.1"
                     /translation="MTELAAAARPVPSRAEGALLPAVDPVVSLPIVTLLPFSRCNCRC
                     AMCDIWKERNGAALSRHDVERLLPDLARLGTRRVVLSGGEPLMHPDLFGLVAPLREAG
                     IGLTLLSSGLLLERHAPAIAATFDDVVVSLDGPAAVHDAIRGVPGAFERIRRGVRALR
                     GGAPVPALSGRCTVQRENLAELRATVAAARDLGLDRISFLAADTTSPAFGRASGWEEA
                     SRLRVVPDADDLARLAAELDRLEEENGGDLASGFVSESAAKLRARLLAHFRAVGSGAP
                     FPASSCNAPWVSAVVETDLSVRPCFFHPAYGRLGDGGLAAVLNGDPALRFRGALDVAS
                     DPVCLRCPCRLNLRDRAGGTT"
     gene            4530..5963
                     /locus_tag="P5164_10055"
     CDS             4530..5963
                     /locus_tag="P5164_10055"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY44278.1"
                     /translation="MSRRRVVLYNPRAVFFTMPLGLLAVGSHLDRSRYEVVLVDGRLE
                     TDPVAAVLAAIGGDHGREAPFVLGVSVLTGSPIQDALEVSRAAKARHPELTVVWGGWH
                     PSLFPEGCLEEPSVDVTVQAQGEETFAEIVRRLDAGESLEGCAGCTHREEGGVPRREP
                     PRPLAPLTDFVSHDYGLLPVERFFALKGKRQLDVVTSQGCRFRCAFCADPFVFGRKWV
                     GLPPERVGEELESLHRRFAFADVNFQDETFFTSLERVAGIAEELIRRRLPSTWAATMR
                     ADQGDRLPDDAWAACKRSGLRRLLIGVEAGTQEMVDRIRKDIRLEQVLRCAERCLRHG
                     VAAQFPFIVCFPGESDASVEATLDFARRLAAMSPTFQTPVFFFKPYPGTPLTEDAVKH
                     GFRLPATLDEWARFDFYAGDGPFTTPARTRLVERFRWYEKHAYGAAPAWKRPVAALAR
                     WRCERRAFRFPVEKAVGELLFPEPVLS"
     gene            complement(5976..7166)
                     /locus_tag="P5164_10060"
     CDS             complement(5976..7166)
                     /locus_tag="P5164_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRY44279.1"
                     /translation="MSPHAPPTCLTGARIVGPGGARRTELRLEGGRVAGDAAHRTGDL
                     VVPLDGHLLLPGLLNAHEHLGLNAFPEADAGGPRASAYEWIDALQPLLAAGSFRDVKR
                     VDEAVRARHGALKNLLSGVTTVAHHDPWLAEMGDPSFPVRVVRPYGWCHSLRLAGRYG
                     PTVEEALAATPPGARFFVHLAEGTDAEAAGELFRLDRLGGLGPATVLVHGVGLGEAGV
                     ARTIERGAAVAWCPASNLRVLGATLDPRPLLAARRLALGSDSRLSGASDLLAELGVAR
                     ENAGLAPEELLRLVTTDAAAVLGRPDAGRLSAGAHADLVVVRDEGGDPTEALTDLPRA
                     RLRAVVLGGVPRVADADLLPWLEAAGVPVRHVLLDGAPKVVDARLFTEEASALEPGLS
                     SAAE"
     gene            complement(7188..7856)
                     /locus_tag="P5164_10065"
     CDS             complement(7188..7856)
                     /locus_tag="P5164_10065"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRY44280.1"
                     /translation="MPIKVLAPAEAYLRWAPTYEEAGALAELDELAVRRLDPAPAGPL
                     LDAACGTGRRLRNADGTFRRGAFGIDLVLPMLLAGARGAAVAAADLTALPFAAGSFRT
                     AWCRLAIGHVAEAGRVYAQLGRALAPGGRLVVTDFHPDAARRGLVRSFRSGEELLAVE
                     HHIHEVDVHRRAAADAGLSLETLLEQPAGEEVRAHFEAAGKLDAWEAQRELPVLLALS
                     FLRL"
     gene            7913..>8516
                     /locus_tag="P5164_10070"
     CDS             7913..>8516
                     /locus_tag="P5164_10070"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HRY44281.1"
                     /translation="MTLPLVLLVNPRVVRRRNARLPLSLLTLAAGLEGKARVRIVDGN
                     FDDDATATVLSAVAAERPALLGVTVMPGPQVAPAIALSKAVRERNPSLPIVWGGYFPT
                     LYPDAALAAPYVDVVVRGPGEATLADLLAALGGTGRAPSLADVAGISWRDGDRAVHNA
                     ERPLGPPDALPPLPYERLGDVSRFLQPTYLGRRTAVHQAAI"
BASE COUNT         1024 a         3288 c         3085 g         1119 t
ORIGIN      
        1 ctcgcgcgcg tcgccccgcg ggtcgtcgcg atcctcgagg acaacttcaa cttcctgacg
       61 aagatgtgca cccagcggag ccgcgaggcg tccgtgcggc tgatccggag cgcgaaggcg
      121 gccggcgcgc gcgtcgccgt gaacgggtcc gacgcttccg acgacccggc gttctacctc
      181 gacgtcggcg ccgacgccgt gatcctcggc gagacggagc ggacgatcgt cgagctcctc
      241 gacgcgtggg accgctcccc cgacgccgac ctgtcggaga tcgcgggcct cgcgctcccc
      301 gcgggcgccg tccgcgcggc cggcagcgcc ccgacccgga ccccgccgcg ccccttcgtc
      361 gcggacctcg acgcccttcc cctccccgcg tgggacctgg tggacgtgga gcgctaccgg
      421 aaggcgtgga cggaggcgca cgggcgcctc tcgtggaaca tggtgacgac ccgcggctgc
      481 ccctaccgct gcaactggtg cgcgaagccg ctctacgggg tccgctacgc gcagcggagc
      541 ccggagctgg tcgccgccga gatgaagctc ctgaaggaga ccgtcggtcc cgaccacgtc
      601 tggttcgcgg acgacatctt cggcctttcc caccgctgga cggagcggtt cgcggaggag
      661 gtccgggagc tcgacgcctc catcccgttc acgatccagt cccgcgtcga cctgatgtcg
      721 ccgaccgcgg tcgaggcgct ccgccgcgcc ggggccgagg aggtctggat gggggtcgag
      781 tcgggggccc agtcgatcct cgacgcgatg gacaagggga cgacgatcgc ccaggtgcgc
      841 ggggcgaccc gccggctgaa ggaggcgggc atccgggcct cgtggttcct ccagctcggc
      901 taccccggcg agacgtgggc cgagatcctc gcgacgcgcg agctcgtccg cgaggagcgc
      961 ccctccgacg tcggcgtctc ggtcgcctat cccctccccg ggacggtctt ctacgagcgc
     1021 gtgaagagcc agctcggcga gcggtcgcac tggaaggaca gcgacgacct cgacatgatg
     1081 ttcgagggga cgtacgccgc gccgttctac aagagggtcc gggacctcct ccacgaggag
     1141 gccgccctcg gcgcgatctc gccgtccgag acgatcggcc ggcgccgccg gaacctcgac
     1201 gccgcgtggc tcgcggcgga gcgcgacgag ccgcgctcgc gcaccggcgg cgcggcgctc
     1261 cccacccggc agcggggctg agggacggcc cccggggcga gccgatgacc gacctcctcc
     1321 tttcgcacgg ctacttcctc gcggaggacg agaaggagcg ggcggtcatg aagccctacc
     1381 cgccgctcgg cctcctctcg ctctcggcct acctgaagcg ggaggggttc tcggtcgagg
     1441 tcttcgactc gacgttcgcg acgcgcgagg cgctctgcgc gcggctcgac gaagggcccg
     1501 gcgtcctcgg cctctacacg aacctgatga cgcgcccgcc cgtcctctcg atcacggccg
     1561 cggcgaaggc gcgtggctgg acggtcgtcc tcggtggccc cgagagcgcg aactacccgg
     1621 ccgagtacct cgccgccggc gccgacgtcg tcgtccgggg cgagggagag gagacgctcc
     1681 gcgagctcct tccggcgctc gccgcgcgag gccctcacca actcgatggg atcgccggcg
     1741 tcgccttccg cgacgaggag ggcgccgtcg tctccggccc gccgcgcgcg caggtccgct
     1801 cgctcgacac gctcccctgg cccgaccgcg aggcgatcga ccacgggccg tacctggcgg
     1861 cctggcggga gcggcacggc gagggaagcg tcaacctcat cacggcgcgc ggctgcgcct
     1921 acaagtgcga ctggtgctct cacgccgtct tcggcaacac ccaccggcgg cgctccccgg
     1981 cgaacgtcgc cgccgaggtg gagtggatcc gcgaccgcta ccgccccgag caggtctggt
     2041 acgccgacga cgtcttcacg atctcgcacc gctggctctt cgagtacgcc ggggagctcg
     2101 agaagcgcgg cctgaagttc ccgttcgaga cgatctcgcg ggccgaccgg atggccgacg
     2161 aggaggtcct cgcgacgctc gcgcgcctcg gctgccgacg gatctggatc ggctcggaga
     2221 gcgggagcca gcgcctcctc gacgcgatgc gccgcggcgt gacggtcgag caggtgcggt
     2281 gggcgacgca cgccgcccgg cgccacggca tcgaggtcgg gatgttcctg atgtggggct
     2341 acgacggcga gaccgccgag gacgtcgagg cgaccgtcga gcacgtccgc gcctcgctcc
     2401 ccgacgtcta cttcacgacg ctcgcctacc cgatcatggg gaccggctac tacgagaagg
     2461 tgaaggggcg ggtctcgctc tcgcggccgt gggccgaagg gggcgaccgt gaccacgtcg
     2521 tcgcgggacg cgcgggccgc gacacctacc ggctcgcaga ccgctggctc cgcgccgccg
     2581 tcgagtcgcg gcggctcgag gagagggcgc cggaagaggc ccgcgcgctc gccggcgagg
     2641 cggaggcggc ccgaagggaa ttcctgtctg cgttcgcgga gggagccgcg tgatcgccgc
     2701 cgcgagcccc ttcgacgccc tcgccgcgac gtacgacgag gacttcaccc ggaccgacct
     2761 cggccggcgc ctccgatcgg ccgtctggcg ccacctcgac gcgacgttcg cgccgggcgc
     2821 gcgggtcctg gagctcggct gcgggaccgg ggaggacgcg ctccacctcg cctcgcgcgg
     2881 cgtgagggtc gtcgcgaccg acgcctcggg agagatggtc gccgagaccg tccggaaggc
     2941 gggggcggcg aacctctcgc acctcgtcga ggcgcgccgc gtcccgttcg aggagctcga
     3001 cgccgccggg ccgtcgctcg gcacgttcga cggcgccttc tcgaacttcg gcgcgctcaa
     3061 ctgcgcggcc gccctcccgc ccgtcgccga ggcgctcgga cgcctcctcc cgtccggcgc
     3121 gcccctcctc ctcgtcgtca tgggtcggtg ggtgccctgg gagtgggcct tctaccttcg
     3181 ccgcggcgac ggagcccgcg cgttccgccg cttacggcgc gacgggaccg tctggcgcgg
     3241 actgaccgtc cgctacccct ctcccggccg gctcctccgg acgttctcac cctggttcct
     3301 gcggaggagg ctggcggcgc tcggtgtctt cctgccgccg agctatgccg cctcctccgt
     3361 ttctccgtcg actctcgccc gcctcgatcg ctgggagagg cggatcgaat ccgcgcctct
     3421 cctcccgcgc ctcgccgacc acacgctctt cgtcctggag aggcgatgac cgagctcgcc
     3481 gcggcggcgc ggcccgtccc gtcgcgcgcg gagggggcgc tcctccccgc cgtcgacccg
     3541 gtcgtctcgc tcccgatcgt cacgctcctg cccttctcgc gctgcaactg ccggtgtgcg
     3601 atgtgcgaca tctggaagga gcggaacggc gccgcgctct cgcgccacga cgtcgagcgg
     3661 ctcctccccg acctcgcacg cctcgggacg cgtcgcgtcg tcctctcagg cggcgagccg
     3721 ctgatgcatc ccgacctctt cggcctcgtc gcgccgctcc gggaggcggg gatcggtctg
     3781 acgctcctct cctccggcct cctcctcgag cggcacgcgc cggcgatcgc ggcgacgttc
     3841 gacgacgtcg tcgtgagcct cgacggcccg gccgcggtcc acgacgcgat ccgcggcgtg
     3901 ccgggggcgt tcgagcggat tcgccgcggc gtccgcgcgc tccgcggcgg cgctcccgtc
     3961 ccggcgctct cggggcgctg cacggtccag cgggagaacc tcgctgagct tcgcgcgacc
     4021 gtcgcggccg cgcgcgacct cggcctcgac aggatcagct tcctcgcggc cgacacgacg
     4081 agcccggcgt tcggccgcgc ttcgggatgg gaggaggcga gccgcctccg cgtcgtcccc
     4141 gacgccgacg acctcgcgcg gctcgccgcc gagctggacc ggctcgagga ggagaacgga
     4201 ggcgacctcg cctccggctt cgtctccgag agcgcggcga agctccgcgc gcgcctcctc
     4261 gcgcacttcc gcgccgtcgg cagcggcgca cccttccccg cctcctcgtg caacgcgccg
     4321 tgggtctcgg cggtcgtcga gaccgacctc tccgtccgcc cctgcttctt ccatcccgcc
     4381 tacggacggc tcggcgacgg cggcctcgcc gcggtcctga acggcgaccc ggcgctccgc
     4441 ttccgaggcg cgctcgacgt cgcttccgac cccgtctgcc tccgctgtcc ctgccgcctg
     4501 aacctgcgcg accgcgcggg agggacgacg tgagccgccg ccgcgtcgtc ctctacaacc
     4561 cgcgggcggt cttcttcacg atgccgctcg gcctcctcgc cgtcggctcg cacctcgacc
     4621 gctcgcggta cgaggtcgtc ctcgtcgacg ggcggctcga gacggacccg gtcgcggccg
     4681 tcctggccgc gatcggcgga gaccacggcc gcgaggcgcc cttcgtcctc ggcgtctcgg
     4741 tcctgacggg gtcgccgatc caggacgcgc tcgaagtctc ccgcgccgcg aaggcgcgcc
     4801 acccggagct gacggtcgtc tggggcggct ggcacccgtc gctcttcccg gaggggtgcc
     4861 tcgaggagcc gtccgtcgac gtcacggtcc aggcgcaggg cgaggagacg ttcgcggaga
     4921 tcgttcggcg gctcgacgcg ggcgagagcc tcgaggggtg cgccggctgc acccaccgcg
     4981 aggagggcgg cgtgccgcgg cgcgagccgc cgcggcccct cgcgccgctc acggacttcg
     5041 tgtcgcacga ctacggcctc ctgccggtcg agcgcttctt cgcgctcaag gggaagcggc
     5101 agctcgacgt cgtcacgtcg caggggtgcc gcttccgctg cgcgttctgc gccgacccgt
     5161 tcgtcttcgg gcggaagtgg gtcgggctgc cgcccgagcg cgtcggcgag gagctcgaga
     5221 gcctccaccg ccggttcgcg ttcgcggacg tgaacttcca ggacgagacg ttcttcacgt
     5281 cgctcgagcg cgtcgccggg atcgccgagg agctgatccg gcggcggctg ccctcgacct
     5341 gggccgcgac gatgcgcgcc gaccagggcg accggctccc cgacgacgcc tgggccgcgt
     5401 gcaagcggtc gggcctccgg cgcctcctca tcggcgtcga ggccgggacg caggagatgg
     5461 tcgaccggat ccgcaaggac atccgcctcg agcaggtcct gcggtgcgcc gagcgctgcc
     5521 tccgccacgg cgtcgccgca cagttcccgt tcatcgtctg cttccccggc gagagcgacg
     5581 cgagcgtcga ggcgacgctc gacttcgcgc gccgcctcgc ggcgatgagc cccacgttcc
     5641 agacgcccgt cttcttcttc aagccctatc ccggcacgcc gctcaccgag gacgccgtga
     5701 agcacggctt ccgcctcccc gcgacgctcg acgagtgggc ccggttcgac ttctacgccg
     5761 gagacggtcc gttcacgacg cccgcgcgga cgcgcctcgt cgagcgcttc cgctggtacg
     5821 agaagcacgc ctacggcgcg gccccggcct ggaagcggcc cgtcgccgcc ctggcccgct
     5881 ggcgctgcga gcggcgggcg ttccgattcc cggtggagaa ggccgtcggc gagctcctct
     5941 tcccggagcc ggtcctctcg tgagccgcct cctcgtcact ccgccgcgga ggaaaggccc
     6001 ggctcgagcg cggaggcctc ttcggtgaag agcctcgcgt cgacgacctt cggcgcgccg
     6061 tcgaggagga cgtgccggac cggaaccccg gccgcctcga gccacggcag gaggtcggcg
     6121 tcggcgaccc tcggcacgcc tccgaggacg acggcgcgga gccgcgccct cgggaggtcc
     6181 gtcagcgcct ccgtcgggtc gcccccctcg tcgcggacga cgacgagatc cgcgtgcgcc
     6241 ccggccgaga ggcgcccggc gtcgggccgg ccgaggacgg cggcggcgtc ggtcgtcacg
     6301 agccgcagga gctcttccgg cgcgagcccg gcgttctccc gcgcgacgcc cagctccgcg
     6361 aggaggtcgc tcgcgccgct cagccgcgag tcgctcccga gggcgagccg cctcgcggcc
     6421 aggagcgggc gcgggtcgag cgtcgcgccg aggacgcgca ggttgctcgc cgggcaccag
     6481 gcgaccgccg cgccccgctc gatcgtgcgc gcgacgcccg cctccccgag gccgacgccg
     6541 tgaacgagga ccgtcgccgg gccgaggccg ccgaggcgat cgaggcggaa gagctctccc
     6601 gcggcctccg cgtcggtccc ctccgcgagg tgaacgaaga agcgagcgcc cggcggcgtc
     6661 gccgcgagcg cctcctcgac cgtcggcccg tatctccccg cgagccgcag cgagtggcac
     6721 cagccgtacg gacggacgac gcggaccggg aaggacgggt cgcccatctc cgcgagccac
     6781 gggtcgtggt gcgccaccgt cgtgacgccg gagaggaggt tcttcagcgc gccgtgccgc
     6841 gcgcggaccg cctcgtcgac ccgcttcacg tcccggaagg aaccggccgc gaggagcggc
     6901 tggagcgcgt cgatccattc gtacgcgctc gcgcgggggc cgccggcgtc cgcctcgggg
     6961 aacgcgttca ggccgaggtg ctcgtgcgcg ttcagcagac ccgggaggag gagatgcccg
     7021 tcgagcggca cgacgagatc gcccgtgcga tgcgccgcgt cgcccgcgac gcggcccccc
     7081 tcgagccgga gctccgtgcg gcgcgcgccg ccggggccga cgatgcgcgc tcccgtgagg
     7141 caggtcggtg gagcgtgagg gctcatcgcg gttccggcag tcccgcgtca gaggcggagg
     7201 aaggagagcg cgaggaggac gggaagctcc ctctgcgcct cccacgcgtc gagcttcccc
     7261 gccgcctcga agtgggcccg cacctcttcg ccggccggct gctcgaggag cgtctcgaga
     7321 gagagcccgg cgtccgcggc ggcgcggcgg tgcacgtcga cctcgtgaat gtggtgctcg
     7381 accgcgagga gctcctcccc gctccggaag gagcggacga gcccgcggcg cgcggcgtcg
     7441 gggtggaagt cggtcacgac gagccggccg cccggcgcga gggcccgccc gagctgcgcg
     7501 taaacgcgcc ccgcctccgc gacgtgcccg atcgcgaggc ggcaccaggc ggtgcggaag
     7561 gagcctgcgg cgaacgggag cgccgtcagg tccgcggcgg cgacggccgc gccgcgcgcc
     7621 cccgcgagga gcatcgggag gacgaggtcg atcccgaacg cgccgcgccg gaacgtgccg
     7681 tccgcgttcc ggagcctgcg gccggtgccg cacgcggcgt cgaggagcgg accggccggc
     7741 gccgggtcga ggcggcggac cgcgagctcg tcgagctccg cgagagcccc cgcctcctcg
     7801 tacgtcggcg cccagcggag gtacgcctcc gcgggagcga gcaccttgat cggcacgggc
     7861 gcgagtatag gagcgggctt ccgcccgcgc ggacgcggtc gaggacgagg ccgtgacgct
     7921 cccgctcgtc ctcctcgtca acccccgcgt cgtgcgacga cgcaacgcga ggctcccgct
     7981 ctcgctcctg acgctcgccg ccgggctcga ggggaaggcg cgcgtgagga tcgtcgacgg
     8041 gaacttcgac gacgacgcga ccgcgaccgt cctctccgcc gtcgccgccg agcgcccggc
     8101 gctcctcggc gtcaccgtga tgccgggccc gcaggtcgcc ccggcgatcg cgctctcgaa
     8161 ggcggtccgc gagcgaaacc cgagtcttcc gatcgtctgg ggcggctact tcccgacgct
     8221 ctaccccgac gccgcgctgg ccgcgcccta cgtcgacgtc gtcgtccgcg ggccgggcga
     8281 ggcgacgctc gcggacctcc tcgccgcgct cggcggcacg ggccgtgcgc cctcgctcgc
     8341 ggacgtcgcg gggatcagct ggagggacgg cgaccgcgcc gtccacaacg cggagcgtcc
     8401 gctcggacct cccgacgccc tgccgccgct tccctacgag cgcctcggcg acgtctcgcg
     8461 gttcctccag ccgacgtatc tcggacggag gaccgccgtc caccaggccg cgatcg
//
LOCUS       DAOTEZ010000166         8507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5891_length_8507_cov_7.155821, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000166 DAOTEZ010000000
VERSION     DAOTEZ010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8507
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5891_length_8507_cov_7.155821"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..1658)
                     /locus_tag="P5164_10075"
     CDS             complement(225..1658)
                     /locus_tag="P5164_10075"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRY44282.1"
                     /translation="MAENLGRILVVDDEPFARTVVRRMLEVDGWEVSEARSTQEARAL
                     LAGEPEPVALLLDVLLDGESGLEYLERLREEGSLVPVIVMTAMEDVETAVRAMKAGAY
                     DFLVKPVTPERLRTALTHAAERRALLDENRNLRKRVRQEAFGRTLVGESPAMKALFSE
                     MERVVETDIAVCLLGETGTGKELVARWLHENGPRGKGPFVDINCAAIPETLIESELFG
                     HEKGAFTGAAARHKGKLEQADGGTLFLDELGEMAHATQARLLRVLQEKTLVRVGGSET
                     IPYNARLISATQRDLTAAVRQGTFREDLYFRVVVYPLRLPPLRERIEDIALLVHHFIR
                     VFRATTGRPVEGITPEAIVTLEAYTWPGNVRELQNAVFRAVVSARGPFLTTADFPDLS
                     PDRVPRYQPRPYPARPDGPPTPAAGLPKAAIVAPGLVTMEDHERAAIQQALDASNGNV
                     RAAADRLQVARSTLYRRIRALGLTAAS"
     gene            complement(1677..>2184)
                     /locus_tag="P5164_10080"
     CDS             complement(1677..>2184)
                     /locus_tag="P5164_10080"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRY44283.1"
                     /translation="YATPALFPIYFRAARRAMAAYPVPLRRTILLERAAATLLHLAVA
                     AAIVFAGGWERAAWLWAIPVFVVFPPVFTLNRLGQHYDVVPGEPSKWGTRMARSRFWD
                     AVWLWSSYHLEHHYFPTVPFYRLRDLNAALTPFFTREGVRERRYRELVRDWIVRNRAP
                     HTDWSARL"
     assembly_gap    2185..2194
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2195..2626)
                     /locus_tag="P5164_10085"
     CDS             complement(<2195..2626)
                     /locus_tag="P5164_10085"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRY44284.1"
                     /translation="MARGHWARSTEELRKRLATAVPNETLRELHRRSAARHLAVALRQ
                     ALLFAGAFAAGWTFESLWVRVPAAIVLGVCVFDGTILLHEVVHRTVFRSDRPFAYRLL
                     GLLYALPSGLSPTQFTRWHLDHHAELGSATDDPKRAWLSPKR"
     gene            2710..3375
                     /locus_tag="P5164_10090"
     CDS             2710..3375
                     /locus_tag="P5164_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44285.1"
                     /translation="MSEKKNWGSTVLGWFVVKEAGEEASLPEETDVSPAEGEAGATPP
                     PGPPPVTFVKEPPRAVAGQVDFDGVLDAAGVDAGERDLFAKAQALLGSLPPGTDPAVR
                     KQIVEASLKAFGVPVDKIIEAGVESIQALEGYLRKTAAETADTAREVQTIVAGYEQKI
                     AEARALLDRKLQEQAAVQTSCNAKKLEVQKVLEFFGQEEVARVVHSSPKLIEPVPASE
                     RKG"
     gene            3380..4093
                     /locus_tag="P5164_10095"
     CDS             3380..4093
                     /locus_tag="P5164_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010109737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HRY44286.1"
                     /translation="MANVGFFARLGNLWKGFLSIWISDVEKEHPEIAYENAINSMVEK
                     YTKLKTATAAIIRRREDIDERLKKATRELAQTEAELNAAVETAQDDLAVILIQKKNQL
                     EADITEMRADMDTASKDAESAKSSLLQVQGEIKKLKAERDTMLARMQSAQARLKIQEQ
                     LDGLSVDAEVKALENVRDHIKTTVAAANLGKELSETSLDGRLAALKTQAGDVQAKQQL
                     AELKAKRAAAQAAAGQKTM"
     gene            4196..5827
                     /locus_tag="P5164_10100"
     CDS             4196..5827
                     /locus_tag="P5164_10100"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding/OmpA
                     family protein"
                     /protein_id="HRY44287.1"
                     /translation="MKLTPFAKLFVTVVVLGVLGYAFWHYKGDTVRQWAGAEKKGAVE
                     SAGVDSADFDALKGAPADAERGKGSEGVTGAALTGSGKLGRPLVVAINTWAGHAPGVV
                     FNAGMDPNPTSYYKRKYGLDVKFVLLEDPAAKLAAFRKGDVDVMWNTVDNWAREASIL
                     SEQGAKAKSILLQDWSRGGDGIVSLASIKSVEDLRGKKVACTQFTPSHFLLLYLLSQS
                     GLTPEERSGVEKAIVFTQDAPAAAAMFKAQQVDAAVTWEPDLSSAVEARGDEAHVLVS
                     TAAATNIIADTLCARQDLIDSYPETVRDFVHGWFDGIEMMKSDPAGSYAVVGRALKLD
                     EETVSGMLSGLKLTPYADNAQFYGLAGGKAHYETLFDTAFVIWRKKGLVTRPVDAKDW
                     ADTRFLGALAAAYQTQKVEEPKVAAKAPSATDRAIINKQIQIHFTPNSDEIMPGSFFV
                     LDALGETMTSFGNTYLRVEGNTDATGKASANMLLSERRALAVKNYVLTNFPNIEPARF
                     QTIGRGSANPVADNTTEAGRQLNRRTDIKVVLATN"
     gene            5829..6626
                     /locus_tag="P5164_10105"
     CDS             5829..6626
                     /locus_tag="P5164_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY44288.1"
                     /translation="MSSRGLFALRAPLPKKTALVLGLVMPTLVLAVWCVLSYGGLAPA
                     DFLPSPSEVVRGTLQLFLQHDLWRAILVSTRRIALAFLIASAVALPLGVLMGAFTPVN
                     RLFEPIVAPLRYMPISAFIPLLILWFGIYEKQKIAFLFLGVFVYLLPVVVTAIRAVPE
                     EYVQTALTLGASRGQVIRTVLLPAAAPEIFDSFRVMNAISWTYVILAEAVNPEHGLGY
                     MVELARTHQKASWSFAGLLVIGGIGLLTDFLITSLSRLLFRWREVSS"
     gene            6623..7426
                     /locus_tag="P5164_10110"
     CDS             6623..7426
                     /locus_tag="P5164_10110"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY44289.1"
                     /translation="MSAVPGAVPRLSVKDLSVTYIGRGGEKTEAVRDVSFDILDKPGH
                     GEIVVFLGPSGCGKSTILKAVAGLLPPTKGDVLLDGQPIGGPSQDRGMVFQAYTSFGW
                     LTVQENVEYGLKLRGVDAKERGDRAREVLKEVGLLDFAKRFPKDLSGGMKQRVAIART
                     LVNKPRVVLMDEPFGALDPMTRWGMQGLLLDVSRIEDNTILFVTHDVSEAVYLADTCY
                     VLSSRPARILHRVDVPAFAERDVHLKSTDEFRAIEKRLLDMLYAPQPGA"
     gene            7436..8362
                     /locus_tag="P5164_10115"
     CDS             7436..8362
                     /locus_tag="P5164_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44290.1"
                     /translation="MAEQQVPRPRTTAQKPAARSGISPAVRDAARETAVGLLKGKAVD
                     TAFGEQPSYLKAAFLNPYNLSLLGGAAAASLLTLNPLPILGAIGLETLWLLWAPDSKR
                     LRHLLWDPRFSKLRKAIEAEERRKRIAGLPDHDRERVEGLVTRREEIESLAAQNPTFT
                     GELLRGELVKTDRLVDAFVEMALTCARYRSYLDSVDARALERDVERYAAEVRRGDPAD
                     QRTEISQRNLAIVVKRQERMREIDHYLAVARGQLDLIENTFQLIADQIVTMQSPKELA
                     GQLDELLEGVEAIKQSARDTEAMLEASGLSNP"
BASE COUNT         1338 a         3014 c         2962 g         1183 t
ORIGIN      
        1 cttgagctgg atctccttgt cgtcgatccg gcgcgaggtg tacgcgatcc ggaaggcacg
       61 gacgaggtcg tccggcagga ggccgtggag cttctccagg ggcggggtcg agacttcgat
      121 cgccatcgtc atcctcggcg tcgggagtgg gcgcccaagg gccgccccgg acgggacggg
      181 agagtagcag agcgggtttg ggcgccgtcg cgggcgcccg gcgctcagga cgcggcggtc
      241 aggcccaggg cgcggatccg ccggtagagc gtcgagcggg cgacctggag gcggtccgcg
      301 gccgcccgga cgttgccgtt cgaggcgtcg agagcctgct ggatggcggc ccgctcgtgg
      361 tcctccatcg tgacgaggcc cggcgcgacg attgccgcct tcgggaggcc ggcggcgggc
      421 gtcggggggc cgtcggggcg ggccggatag gggcgcggct ggtagcgcgg cacccgatcg
      481 ggggagaggt ccgggaagtc ggccgtcgtc aggaacggcc cccgtgccga gacgacggcc
      541 cggaagacgg cgttctgaag ctcgcgaacg ttccccggcc aggtgtacgc ctcgagcgtc
      601 acgatcgcct cgggcgtgat cccctcgacg gggcggccgg tggtcgcccg gaagacccgg
      661 atgaagtggt ggaccaggag ggcgatgtcc tcgatccgct cccggagcgg ggggagccgc
      721 agcggataga cgacgacccg gaagtagagg tcctcccgga acgtcccctg ccggacggcg
      781 gccgtcaggt cgcgctgcgt cgccgagatc agccgggcgt tgtaggggat cgtctcgctc
      841 cccccgaccc gcaccagggt cttctcctgg aggacgcgga ggaggcgggc ctgggtggcg
      901 tgggccatct ccccgagctc gtcgaggaag agcgtcccgc cgtcggcctg ctcgagcttc
      961 cccttgtgcc gggcggcggc gccggtgaac gcccccttct cgtgcccgaa gagctcgctc
     1021 tcgatcagcg tctccgggat cgccgcgcag ttgatgtcga cgaacgggcc cttgccgcgc
     1081 gggccgttct cgtggagcca gcgggcgacg agctccttgc ccgtacccgt ttccccgagg
     1141 aggcagacgg cgatgtcggt ctcgacgacc cgctccatct cggagaagag cgccttcatc
     1201 gccgggctct cgccgacgag ggtccggccg aaggcctcct ggcggacccg cttgcggaga
     1261 ttccggttct cgtccaggag cgcgcggcgc tcggcggcgt gcgtcagggc cgtccggagc
     1321 ctctccggcg tcaccggctt gacgaggaag tcgtaggcgc cggccttcat cgcccggacg
     1381 gcggtctcca cgtcctccat cgcggtcatg acgatgaccg ggacgaggga tccctcctcg
     1441 cgcagccgct cgaggtactc gagcccgctc tcgccgtcga ggaggacgtc gaggagaagc
     1501 gcgaccggct ccggctcccc ggcgaggagc gcccgggcct cctgcgtcga ccgggcctcg
     1561 gagacctccc agccgtccac ctcgagcatc cgccggacga ccgtccgcgc gaaaggctca
     1621 tcgtcgacga cgaggatcct gccgaggttc tccgccataa agggttgcca agtctattac
     1681 agccgggcgc tccagtccgt gtggggcgcc cggttcctca cgatccagtc ccggacgagc
     1741 tcccggtagc gccgctcgcg gaccccttcc cgcgtgaaga acggggtcag ggcggcgttc
     1801 aggtcgcgca gcctgtagaa ggggacggtc gggaagtagt ggtgctcgag gtggtagctc
     1861 gaccagagcc agaccgcgtc ccagaagcgg gagcgggcca tccgggtccc ccacttcgac
     1921 ggctcgcccg ggacgacgtc gtagtgctgc cccagccggt tcagagtgaa gaccggcggg
     1981 aagacgacga agaccgggat cgcccagagc catgccgccc gttcccaccc gccggcgaag
     2041 acgatcgccg ccgcgacggc gaggtggagg agcgtcgcgg ccgcccgctc gaggaggatc
     2101 gtccggcgga gcgggacggg gtaggccgcc atcgcgcgcc gggccgcccg gaagtagatc
     2161 gggaagagcg ccggcgtcgc gtagnnnnnn nnnnccgctt cggcgagagc caggccctct
     2221 tcgggtcgtc cgtcgccgag ccgagctcgg cgtggtggtc gaggtgccag cgcgtgaact
     2281 gcgtcggcga cagccccgac ggcagggcgt agaggagccc caggaggcgg tacgcgaacg
     2341 gccggtcgga gcggaagacg gtccggtgga cgacctcgtg gaggaggatt gtcccgtcga
     2401 agacgcagac cccgaggacg atcgccgcgg gcacccggac ccagaggctc tcgaacgtcc
     2461 agccggcggc gaaggccccg gcgaagagga gcgcctggcg gagggcgacg gcgaggtgcc
     2521 gcgcggcgga cctgcggtgc agctcccgca gcgtctcgtt cggcaccgcc gtcgcgagac
     2581 gcttccggag ctcctccgtc gaccgggccc agtgaccgcg cgccatctct cgtccgactt
     2641 tacgacggcc cggacgggga gcggattccg ggccgtccgc ttgataagat ctcgaattcg
     2701 ggacttgcca tgagcgaaaa gaagaactgg ggctcgaccg tcctcggctg gttcgtcgtc
     2761 aaggaggcgg gggaagaggc ttctctcccc gaggagacgg acgtctcccc ggcggaaggg
     2821 gaggccggcg ccacgccccc tcccggcccg ccgcccgtca cgttcgtgaa ggagccgccg
     2881 agagcggtcg ccggacaggt cgacttcgac ggcgtcctgg acgccgcggg ggtggacgcg
     2941 ggagagcgcg acctcttcgc gaaggcgcag gcgctcctcg ggagcctccc gccggggacc
     3001 gatccggcgg tgcggaagca gatcgtcgag gcgtcgctga aggcgttcgg cgtccccgtc
     3061 gacaagatca tcgaggcggg cgtcgagtcg atccaggctc tcgagggcta cctgcgaaag
     3121 acggcggccg agacggccga cacggcgcgc gaggtccaga cgatcgtcgc cgggtacgaa
     3181 cagaagatcg ccgaggcgcg cgccctcctc gacaggaaac tgcaggagca ggcggccgta
     3241 cagacgagct gtaacgcgaa gaagctcgag gtccagaagg tcctcgagtt cttcgggcag
     3301 gaggaggtgg cgcgggtggt ccattcgtcg ccgaagctca tcgagccggt ccccgcgtcc
     3361 gagaggaagg gctgaaagca tggccaacgt cggtttcttc gcgcggctgg gcaacctctg
     3421 gaaggggttc ctctcgatct ggatctcgga cgtcgagaag gagcacccgg agatcgccta
     3481 cgagaacgcc atcaactcga tggtggagaa gtacacgaag ctcaagacgg cgacggcggc
     3541 catcatccgg cgccgcgagg acatcgacga gcgcctgaag aaggcgacgc gcgagctcgc
     3601 gcagaccgag gccgagctga acgcggcggt cgagacggcg caggacgacc tcgccgtcat
     3661 cctgatccag aagaagaacc agctcgaggc cgacatcacc gagatgcggg ccgacatgga
     3721 caccgcctcg aaggacgccg agtcggcgaa gagctcgctc ctgcaggtgc agggcgagat
     3781 caagaagctg aaggccgagc gcgacacgat gctcgcgcgg atgcagtcgg cgcaggcgcg
     3841 cctgaagatc caggagcagc tcgacggcct ctcggtcgac gccgaggtga aggcgctcga
     3901 gaacgtccgc gaccacatca agacgaccgt cgccgccgcg aacctcggca aggagctctc
     3961 ggagacctcg ctcgacggcc gcctcgcggc gctcaagacc caggccggcg acgtccaggc
     4021 caagcagcag ctcgccgagc tgaaggcgaa gagggccgcc gcgcaggcgg ccgccggcca
     4081 gaagacgatg tagatccgac gggccccttc gcgggggccc gtcgcgtttc gcgcggaggg
     4141 gcccgcggcg cgatccggcg ccggagcggc cgtcccctcc gccagggaga gggaaatgaa
     4201 gctgaccccg ttcgccaagc tcttcgtcac cgtggtcgtc ctcggcgtcc tcgggtacgc
     4261 cttctggcac tacaagggag acaccgtccg gcagtgggcg ggcgccgaga agaagggggc
     4321 cgtcgagagc gccggcgtcg actccgccga cttcgacgcg ctcaagggcg ccccggccga
     4381 cgccgagcgc ggcaagggct ccgagggcgt cacgggcgcc gcgctcacgg gctccggcaa
     4441 gctcggccgg ccgctcgtcg tcgccatcaa cacctgggcg gggcacgccc ccggcgtcgt
     4501 cttcaacgcc ggcatggacc cgaacccgac ctcgtactac aagcggaagt acgggctcga
     4561 cgtgaagttc gtcctcctcg aggacccggc cgcgaagctg gccgcgttcc gcaagggcga
     4621 cgtcgacgtc atgtggaaca cggtcgacaa ctgggcgcgc gaggcgtcga tcctctcgga
     4681 gcagggagcg aaggcgaagt cgatcctcct gcaggactgg tctcgcggcg gcgacggcat
     4741 cgtctcgctc gcgtcgatca agtcggtcga ggacctgcgc ggcaagaagg tcgcctgcac
     4801 ccagttcacg ccgtcccact tcctcctcct ctacctcctc tctcagtcgg ggctcacccc
     4861 cgaggagcgc tccggcgtcg agaaggcgat cgtcttcacg caggacgccc ccgccgccgc
     4921 cgcgatgttc aaggcgcagc aggtcgatgc cgccgtgacc tgggagcccg acctttcctc
     4981 cgccgtcgag gcgcggggcg acgaggcgca cgtcctcgtc tccacggccg ccgccacgaa
     5041 catcatcgcg gacacgctct gcgcccggca ggacctgatc gactcctacc ccgagacggt
     5101 ccgggacttc gtccacggct ggttcgacgg gatcgagatg atgaagtcgg acccggcggg
     5161 ctcctacgcg gtggtgggcc gggccctgaa gctcgacgag gagacggtct ccggaatgct
     5221 ctcgggcctc aagctgaccc cctacgcgga caacgcccag ttctacggcc tcgcaggggg
     5281 caaggcgcac tacgagacgc tcttcgacac cgcgttcgtc atctggcgca agaagggcct
     5341 cgtcacgcgg ccggtcgacg cgaaggactg ggccgacacc cgcttcctcg gcgccctcgc
     5401 cgccgcctac cagacgcaga aggtcgagga gccgaaggtc gccgcgaagg cgccgtcggc
     5461 gacggaccgg gcgatcatca acaagcagat ccagatccac ttcacgccga acagcgacga
     5521 gatcatgccg ggctccttct tcgtcctcga cgccctcggc gagacgatga cctcgttcgg
     5581 gaacacctac ctgcgcgtcg agggaaacac cgacgcgacc ggcaaggcct ccgcgaacat
     5641 gctcctttcc gagcggcgcg ccctcgccgt gaagaactac gtcctgacga acttcccgaa
     5701 catcgaaccg gcccgcttcc agacgatcgg gcgcggctcg gcgaacccgg tcgccgacaa
     5761 cacgaccgag gccggacggc agctgaaccg ccggaccgac atcaaggtcg tcctggcgac
     5821 gaactgagtt gagcagccgc gggctcttcg cgctccgggc gccgcttccg aagaagacgg
     5881 cgctcgtcct cggcctcgtc atgccgacgc tcgtcctcgc cgtctggtgc gtcctgagct
     5941 acggcggcct cgcgccggcc gacttcctcc cgtcgccgtc cgaggtcgtc cgcggcacgc
     6001 tccagctctt cctccagcac gacctctgga gggcgatcct cgtctccacc cgccggatcg
     6061 cgctcgcgtt cctcatcgcc tcggcggtcg ccctcccgct cggcgtcctg atgggagcgt
     6121 tcaccccggt gaaccgcctc ttcgagccga tcgtggcccc gctccgctac atgccgatct
     6181 cggccttcat cccgctcctc atcctctggt tcgggatcta cgagaagcag aagatcgcgt
     6241 tcctcttcct cggggtcttc gtctacctcc tcccggtcgt cgtcacggcg atccgggccg
     6301 tcccggagga gtacgtccag accgcgctca ccctcggagc ctcgcgcggc caggtcatcc
     6361 ggacggtcct cctcccggcg gcggcgcccg agatcttcga ctccttccgc gtcatgaacg
     6421 ccatctcgtg gacctacgtc atcctcgccg aggcggtgaa ccccgagcac ggcctcggct
     6481 acatggtcga gctcgcgcgg acgcaccaga aggcgtcgtg gtcgttcgcc ggcctcctcg
     6541 tcatcggcgg catcggcctc ctgaccgact tcctgatcac ctcgctctcg cgcctccttt
     6601 tccggtggcg ggaggtgagc tcgtgagcgc cgttcccggc gccgtcccgc gcctctcggt
     6661 gaaggacctc tccgtcacgt acatcgggcg gggcggcgag aagaccgagg cggtgaggga
     6721 cgtctccttc gacatcctcg acaagccggg ccacggcgag atcgtcgtct tcctcggccc
     6781 ctccgggtgc ggcaagtcga cgatcctgaa ggccgtcgcc ggcctcctcc cgccgacgaa
     6841 gggcgacgtc ctcctcgacg gccagccgat cggcggcccc tcgcaggacc gcggcatggt
     6901 cttccaggcc tacacgtctt tcggctggct gacggtccag gagaacgtcg agtacggcct
     6961 gaagctccgc ggcgtcgacg cgaaagagcg cggcgaccgc gcccgcgagg tgctgaagga
     7021 ggtcgggctc ctcgacttcg cgaaacgctt cccgaaggac ctctcgggcg ggatgaagca
     7081 gcgggtcgcg atcgcgcgga ccctcgtgaa caagccgcgc gtcgtcctga tggacgagcc
     7141 gttcggcgcc ctcgacccga tgacgcgctg ggggatgcag ggcctcctcc tcgacgtctc
     7201 ccggatcgag gacaacacga tcctcttcgt cacgcacgac gtctccgagg cggtctacct
     7261 cgccgacacc tgctacgtcc tctcctcgcg tccggcgcgg atcctccacc gggtcgacgt
     7321 cccggccttt gccgagcgcg acgtccacct gaagtcgacg gacgagttcc gggcgatcga
     7381 gaagaggctc ctcgacatgc tctacgcccc gcagccgggg gcgtagaccg gcacgatggc
     7441 ggagcagcag gtcccccggc cgaggaccac ggcgcagaag cccgcggccc gctcggggat
     7501 ctctcccgcc gttcgggacg ccgcgcggga gacggcggtc ggcctcctga aggggaaggc
     7561 cgtcgacacg gccttcggcg agcagccctc gtacctgaag gcggcgttcc tcaaccccta
     7621 caacctctcc cttctcggcg gggcggccgc ggcgtcgctc ctgactctga acccgctccc
     7681 gatcctcggg gcgatcgggc tcgagacgct ctggctcctc tgggcgcccg acagcaagcg
     7741 gctccgccac ctcctctggg acccgcgttt cagcaagctc cggaaggcca tcgaggccga
     7801 agagcggcgc aagcggatcg cggggctccc ggaccacgac cgcgagcgcg tcgaggggct
     7861 cgtgacgcgc cgggaggaga tcgagagcct cgccgcgcag aacccgacgt tcacgggaga
     7921 gctcctccgg ggcgagctcg tgaagacgga ccgcctcgtc gacgcattcg tggagatggc
     7981 cctcacctgc gcccgctacc gctcctacct cgactccgtc gacgcgcggg cgctcgagcg
     8041 ggacgtcgag cgctacgcgg ccgaggtccg ccggggagac cccgcagacc agcggaccga
     8101 gatctcgcag cggaacctcg cgatcgtcgt gaagcgccag gagcggatgc gggagatcga
     8161 ccactacctc gccgtcgccc gcggccagct cgacctgatc gagaacacgt tccagctgat
     8221 cgccgaccag atcgtcacga tgcagtctcc gaaggagctc gcgggccagc tcgacgagct
     8281 cctggaggga gtcgaggcga tcaagcagtc ggcgcgcgac acggaggcga tgctcgaggc
     8341 ctccggcctc tcgaacccct gagcaccccc aaggagcccc gaatggccga cgtccgcatc
     8401 ctccccccct gggccgagga cctccgccgc cgctacctgc ggaacgaggc ggtcctcttc
     8461 gtcctccacg gaaacgtcca cgacctcgtc ctcgtcgacg gcgcgct
//
LOCUS       DAOTEZ010000167         8440 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_5956_length_8440_cov_7.119499, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000167 DAOTEZ010000000
VERSION     DAOTEZ010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8440
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5956_length_8440_cov_7.119499"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2016
                     /locus_tag="P5164_10120"
     CDS             <1..2016
                     /locus_tag="P5164_10120"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HRY44291.1"
                     /translation="ELNAEQQAVVFAPDGPTVVVAGAGSGKTRTLVYRVSRLVEDGVA
                     PEAILLLTFTNRAAREMTRRVERLLGADLARMTAGTFHSVGARLLRPHAELLGYRPNF
                     SILDSEDSKDLLESATSDLGIPITERRFPKGDLLRAIVSFAANTGQKLDAVIGRDYPH
                     LLSQLEPVKAVVRRYLERKVLANAMDYDDLLLNWRRLLETHPEVRKALALRFRAVLVD
                     EFQDVNRLQSEIVDLVAQDSEAKNVMVVGDDAQSIYSFRGADAEALLGFPDRYPGATV
                     YRLETNYRSTPEILRLADASIAHNSRRYEKTLVPTRESGVPVAVVGAKDLLQQAEFVA
                     QRVLELRDEGTPLSEIGVLYRAHFQALELQIELTRRGIPYEVRSGLRFFEQAHVKDVL
                     AHLRLLANPKDEMSFKRALKLLPKVGERTAAVLWEAVSDSHEPLAAFLALELGKAPAG
                     ARAGLLRFRETMRTLKKPSYLSSPAEAIRYVVEDGGYGDVAKGKFPNHSARLDDLEAL
                     AQFALAYDDVGRFLEEVTLYGDPSGEDQVAGEEDDERLVLSSVHQAKGLEWKAVFVIG
                     LQEDRFPNLRACKTPEGLEEERRLFYVAATRAKDELTLVHPLTAFDRYGVVVVTEPSQ
                     FLRELDATLYERWVLDESVEASLSPAPERPRFAADPTLVPDDEEPVN"
     gene            complement(2017..2667)
                     /locus_tag="P5164_10125"
     CDS             complement(2017..2667)
                     /locus_tag="P5164_10125"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRY44292.1"
                     /translation="MSRLSRLQEDVLHGFFRRTRSFFLTGGAALAAFHLGHRATDDLD
                     LFTFSADLDEGASALRSVAEELGASIQALRTSPEFRRFLISVGAESVIVDLVRDRAPQ
                     IHVEKLDRDGVLVDPPEEILANKLRTLLSRGEVRDLVDVLVLERAGFRAEDVIGPASS
                     KDGGLTPAQLAWVLSQITVGPDARVPAGITPDELRAFLENLVSRLTRLAWPGAPAS"
     gene            complement(2671..2967)
                     /locus_tag="P5164_10130"
     CDS             complement(2671..2967)
                     /locus_tag="P5164_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44293.1"
                     /translation="MPALSTRLTDPDAIPYFLWDDPMTVSELEERLRTASEPERLRLL
                     ALVLREARDPDVWRFVTPEEVVRLWPRLAGRLGLRRAFWEFLLDRWKRLGLVEA"
     gene            complement(3003..3566)
                     /locus_tag="P5164_10135"
     CDS             complement(3003..3566)
                     /locus_tag="P5164_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44294.1"
                     /translation="MGTVEPPPGQAAARLAPAVASSPGRSPWTIALLVLGALAAFLWF
                     RRPSYLPDVPLPSASAEGVSAGPQASPVFCRGSKGCLVVYVAPWCGPCRSSLPGDVAL
                     ADHIAPLGYETVFVVGMDDVRACLEMTKSIGRPARIDSDRKWARAARVSGVPHFLVTT
                     PTGKIVKRQAGAYLAPPDQVAQLLGIS"
     gene            complement(3617..4450)
                     /locus_tag="P5164_10140"
     CDS             complement(3617..4450)
                     /locus_tag="P5164_10140"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY44295.1"
                     /translation="MLRRPLLSRLGCGAAILAAAALAALMLTPLVRSMIFPASQAPFP
                     EPREGEGWSVVSYETTDGILLAGASFPAPSSDAPVLLYFHGNAEAAAHNLPLADDLRA
                     RGFGVFLAEYRGYGGLCGSPSEEGLYADGEAALAELGRRGISPERVVLVGRSLGSGVA
                     VELATRHRVAAVVLVSAYTSLVDMGRIVAGPLAPLVVRDRFDSLSKVGRVGAPVVLIH
                     GALDDVVPVAMGRRLAAARPGARWVEVPGTSHNDFPGLAALVADEVDAALAGARNAPP
                     P"
     gene            4560..5723
                     /locus_tag="P5164_10145"
     CDS             4560..5723
                     /locus_tag="P5164_10145"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY44296.1"
                     /translation="MNLRLLGTLLLGHLTTDLAQGMIPALLPFFIAERGLSYAAAGGL
                     VLAATVASSIVQPVFGYFADRRPSPWLVPAGVVTAGVGVALAGVAPSYTLIAASVALH
                     GLGVAAFHPEGARAAAFASGPKRATGMSLFSTGGNLGMATGPLVITPLVLALGLRATL
                     VPLLPVVLVAAFLATQTGRLAALRPNAHGPASASADADRWGPFGRLAVVVMSRSVVYT
                     GLSAFLPLFFVGALGRTKAEGATALSILLGTGVVGTLAGGRLADRFGRRPVILASMAA
                     LAPLLFALASARDPRVATALLVPVALALYLPFSVLVVMGQEYLPNHVGTASGVTVGLA
                     VTAGGLAAPLLGLVADRWGVAGPLFVVSALPILAVALTLTLPDSRAEETVARG"
     gene            5997..6449
                     /locus_tag="P5164_10150"
     CDS             5997..6449
                     /locus_tag="P5164_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44297.1"
                     /translation="MERDFVEMLSALDAAGAEYLVVGAHALAAHGVPRATGDLDIWVR
                     ATPENAARVLAALRDFGAPLSDLSPEDLTRPETVFQIGVVPVRVDILTSISGVEFEEA
                     WAGRIQATVDAVTFGVLGREELIRNKRATGRPKDLLDVETLLGEPGAR"
     gene            complement(6504..>8440)
                     /locus_tag="P5164_10155"
     CDS             complement(6504..>8440)
                     /locus_tag="P5164_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44298.1"
                     /translation="ERWDSTRDLARDLAIWGQRGGERSGTAGGATAEPVSATRSSLKR
                     TTLAATGLCVAIAAAVWGAFQAGLVRGRGRSAEPKPIPKLVQLTWEAGMESGPSVSPD
                     GGSFVYVARRAGGSDIFLRRVGGENPTNLTKDFPGNDLQPAFSPDGSTIAFRSERDGG
                     GIFLMGASGESPRRLTDFGYDPAWSPDGGRIVFATTTPGPTAGNQSELWIVDVATGQT
                     TKVYDGDGRTPTWSPSGIRIAFVQGGAGRLLAGSPGLSTIPASGGDPTPLTVPITSTW
                     HPRPDWSRSGITFDSAVGGQVNLWRVGVDETTGKVTDEAAPVVASATVSQTSSSTPDG
                     RRILFGTWIGAYTIRRSDFDPVRGRLIGSPRELVSGPRDLRVTGLSQNGEWLLTVFLE
                     RGGRKDIVLVRVQTGETRRLTDDAPAKDFPRWAPDGSRIYFGIAPDGLNEVWSIRPDG
                     SGRQCELRAAKEGGAFPSVVSPDGRTLYVDVGKEVRPHTVDLSAPPEERRLTPLPLLK
                     DGNLFDTWWASPDGRRILGKSRTPDGRTVSEVHFALDVATGTYESIPTPPKGRLLGWL
                     PDSRRILLARDGKLEVLDRLTGKVTPAGSTAPDGLFSSFCFSPDGRSVYWTTANSEGD
                     IWMLDYGEAAPAEVSTDKRR"
BASE COUNT         1184 a         3068 c         3030 g         1158 t
ORIGIN      
        1 gagctgaacg ccgagcagca ggcggtcgtc ttcgcccccg acggcccgac cgtcgtcgtc
       61 gccggggccg gctcggggaa gacccggacg ctcgtctacc gcgtctcccg cctcgtcgag
      121 gacggggtcg cccccgaggc gatcctcctc ctgacgttca cgaaccgggc cgcccgggag
      181 atgacccgga gggtcgagcg cctcctcggc gccgacctcg cccggatgac cgccggcacg
      241 ttccattccg tcggcgcgcg ccttctgcgc cctcacgccg agctcctcgg ctaccggccc
      301 aacttctcga tcctcgattc cgaggactcg aaggacctgc tcgagtcggc cacctccgac
      361 ctcggcatcc cgatcaccga gcggcgcttc ccgaaggggg acctcctccg cgcgatcgtc
      421 tccttcgcgg cgaacacggg gcagaagctc gacgccgtga tcgggcgcga ctacccgcac
      481 ctcctctcgc agctcgagcc cgtgaaagcg gtcgtccgcc gctacctcga acggaaggtc
      541 ctcgcgaacg cgatggacta cgacgacctc ctcctgaact ggaggaggct cctcgagacg
      601 cacccggagg tccggaaggc gctcgcgctc cgcttccggg cggtcctcgt cgacgagttc
      661 caggacgtga accggctcca gtcggagatc gtcgacctcg tcgcgcagga ctccgaagcg
      721 aagaacgtca tggtcgtcgg cgacgacgcc cagtcgatct actccttccg cggcgccgac
      781 gcggaggcgc tcctcggctt ccccgaccgc tacccgggcg cgacggtcta ccgcctcgag
      841 acgaactatc gttcgacgcc cgagatcctg cggctcgccg acgcctccat cgcgcacaac
      901 tcgcgccggt acgagaagac gctcgtcccg acgcgggaga gcggcgtgcc ggtggccgtc
      961 gtcggcgcga aggacctcct ccagcaggcg gagttcgtgg cgcagcgggt cctcgagctg
     1021 cgcgacgagg ggacgcccct ctccgagatc ggcgtcctct accgcgccca cttccaggcg
     1081 ctggagctgc agatcgagct gacccgccgg ggcatcccgt acgaggttcg ctcggggctc
     1141 cggttcttcg agcaggcgca cgtcaaggac gtcctcgcgc acctgcgcct cctcgcgaac
     1201 ccgaaggacg agatgtcgtt caagcgggcg ctcaagctcc tgccgaaggt gggggagagg
     1261 acggcggccg tcctctggga ggccgtctcc gactcccacg agcctctcgc ggcgttcctc
     1321 gcgctggagc tcggcaaggc gccggccggc gcgcgcgcgg gcctcctccg cttccgcgag
     1381 acgatgcgga cgctgaagaa gccgtcgtac ctctcctcgc ccgccgaggc gatccgctac
     1441 gtcgtggagg acggcggcta cggcgacgtc gcgaagggga agttcccgaa ccactccgcg
     1501 cggctcgacg acctcgaggc gctcgcgcag ttcgcgctcg cgtacgacga cgtcggccgc
     1561 ttcctcgagg aggtgacgct ctacggcgac ccctccggcg aggaccaggt cgcgggggag
     1621 gaggacgacg agcggctcgt cctctcctcc gtccaccagg ccaaggggct cgagtggaag
     1681 gcggtcttcg tcatcggcct ccaggaagac cgcttcccga acctgcgcgc ctgcaagacc
     1741 cccgaggggc tcgaggagga gcgccgcctc ttctacgtcg cggcgactcg tgcgaaggac
     1801 gagctgacgc tcgtccaccc gctgaccgcc ttcgacagat acggcgtcgt cgtcgtcacc
     1861 gagccgagcc agttcctccg ggagctcgac gcgacgctct acgagcgctg ggtcctcgac
     1921 gagagcgtcg aggcgtcgct ttcccccgcc cccgagcggc cccgcttcgc cgccgatccg
     1981 acgctcgtcc cggacgacga agaacctgtg aactagctag gaggcgggcg cgcccggcca
     2041 ggcgagacgg gtcaggcggg agacgaggtt ctcgaggaag gcacggagct cgtcaggcgt
     2101 gatcccggcc ggaacgcgtg catcgggccc gacggtgatc tgcgacagga cccatgcgag
     2161 ctgcgcgggc gtgagccccc cgtccttgct cgaagccgga ccgatgacgt cctccgcgcg
     2221 gaatccggcc cgctccagga ccaggacgtc gacgaggtcc cgaacctcgc cgcgagacag
     2281 gagggtgcgg agcttgttcg cgaggatctc ctctggcggg tcgacgagga ctccgtcccg
     2341 gtcgagcttc tcgacgtgga tctggggtgc ccggtcgcgc acgaggtcga cgatgacgct
     2401 ctctgcgccg acgctgatca ggaatcgccg gaactcgggc gaggtccgga gggcctggat
     2461 cgaggcgccc agctcctcgg cgacggagcg aagcgccgaa gccccttcgt cgagatcggc
     2521 ggagaacgtg aagaggtcga ggtcgtcggt cgccctgtgc ccgaggtgga aggcggcgag
     2581 agccgcgccg cccgtcagga agaacgagcg cgtcctgcga aagaacccgt ggaggacgtc
     2641 ctcctgaagc cggctcaggc gcgacatccg ctacgcctcg acgagcccga gccgcttcca
     2701 gcggtccagg aggaactccc agaacgcgcg gcggaggccg agacgtccgg cgagccgcgg
     2761 ccagaggcgg acgacctctt cgggcgtcac gaaccgccag acgtcggggt cccgggcctc
     2821 gcgcaggacg agagcgagaa gcctcagcct ctcgggctcc gacgccgtgc gcagacgctc
     2881 ctcgagctcc gagacggtca tcggatcgtc ccagaggaag taggggatcg cgtccgggtc
     2941 ggtgagcctc gtggagagcg cgggcacggg agaagtctac ccgcgccccg tctgcgcggt
     3001 cgtcaggaga ttccgaggag ctgcgcgacc tggtccggcg gggccaggta ggcgcccgcc
     3061 tgccgcttca cgatcttccc ggtcggagtc gtcacgagga agtgcggcac gccgctgacg
     3121 cgcgcggccc gcgcccactt ccggtcggag tcgatccgcg ccgggcgacc gatcgacttc
     3181 gtcatctcga ggcaggcgcg cacgtcgtcc atcccgacga cgaagacggt ctcgtacccc
     3241 agcggggcga tgtggtccgc gagggcgacg tcgcccggca gggaggagcg gcaggggccg
     3301 caccacgggg cgacgtagac gacgaggcag cccttcgagc cgcggcagaa gaccggcgac
     3361 gcttgcgggc cggcggagac gccctccgcg gaggccgagg ggagcggaac gtcgggcagg
     3421 tagctcggtc gccggaacca gaggaacgcg gcgagggcgc cgaggacgag gagcgcgatc
     3481 gtccagggag agcggcccgg ggaggaggcg acggccgggg cgagacgcgc ggcagcctgt
     3541 ccgggcgggg gctcgacagt tcccatggcg gaagatcgta gcgcggaagc ccgacgcggc
     3601 cgggcgggcg cggcggctac gggggcggcg cgttccgggc gcccgcgaga gccgcgtcga
     3661 cctcgtcggc gacgagcgcg gcgagcccgg ggaagtcgtt gtggctcgtc cccgggacct
     3721 cgacccagcg ggcgccgggc cgcgccgccg cgaggcgccg ccccatcgcg accgggacga
     3781 cgtcgtcgag cgccccgtgg atgaggacga ccggcgcccc gacgcgcccg accttcgaca
     3841 gggagtcgaa ccggtcccgg acgacgagcg gcgcgagcgg cccggcgacg atccgcccca
     3901 tgtcgacgag cgaggtgtag gccgagacga ggacgacggc ggcgacccgg tggcgcgtcg
     3961 cgagctcgac ggcgaccccc gagccgagcg agcggcccac gaggacgacg cgctcgggag
     4021 agatgccgcg cctcccgagc tcggcgaggg ccgcctctcc gtcggcgtag agcccctcct
     4081 cggacggcga gccgcagagt ccgccgtagc cgcggtactc ggcgaggaag acgccgaagc
     4141 cgcgggcgcg gaggtcgtcc gcgagcggga ggttgtgggc cgccgcctcg gcgttcccgt
     4201 ggaagtagag gaggacgggg gcgtcggacg acggggccgg aaaggaagcg cccgcgagga
     4261 ggatgccgtc cgtcgtctcg tacgaaacga cgctccaccc ttcgccctcg cgcggctccg
     4321 ggaacggcgc ctgcgaggcc gggaagatca tcgagcggac gagcggggtc agcatgaggg
     4381 cggccagggc agcggcggcg agtatggcgg ctccgcagcc gaggcgggag aggagcgggc
     4441 ggcggagcac ggggcgagtg tagggcgggc gtcgcgcggt gcgcccgcgc cgttcgacga
     4501 gccgccccga cgggccccct cgaattgaca tccggccctc ccgcgcgcac aaccccgcgg
     4561 tgaacctccg gctcctcggg accctcctcc tcggccacct cacgaccgac ctcgcgcagg
     4621 ggatgattcc cgcgctcctc ccgttcttca tcgccgagcg ggggctcagc tacgcggcgg
     4681 ccgggggcct cgtcctcgcc gcgacggtcg cctcctcgat cgtccagccc gtcttcgggt
     4741 acttcgccga ccggcgcccc tcgccctggc tcgtcccggc gggcgtcgtg acggccggcg
     4801 tcggcgtcgc cctcgcgggg gtcgcgccgt cgtacacgct cattgccgcc tccgtcgcgc
     4861 ttcacggcct cggcgtcgcc gccttccacc ccgagggggc tcgtgccgcg gccttcgcct
     4921 cggggccgaa gcgcgcgacc ggcatgagcc tcttctcgac gggcggcaac ctcgggatgg
     4981 cgaccggccc gctcgtcatc acgccgctcg tcctcgcgct cggcctccgc gcgacgctcg
     5041 ttccgctgct cccggtcgtc ctcgtcgcgg cgttcctcgc gacgcagacc gggcgcctcg
     5101 ccgcgctccg cccgaacgcg cacggcccgg cgtccgcctc ggccgacgcg gaccgatggg
     5161 gcccgttcgg ccgcctcgcc gtcgtcgtca tgagccgttc cgtcgtctat accggcctct
     5221 ccgcgttcct ccccctcttc ttcgtcgggg cgctcgggag gacgaaggcg gaaggggcga
     5281 cggcgctctc gatcctcctc gggacgggcg tcgtcgggac cctcgccggc ggccgcctcg
     5341 ccgaccgctt cggccggcgc cccgtcatcc tcgcctcgat ggcggcgctc gctccgctcc
     5401 tcttcgccct cgcctcggcc cgcgacccgc gcgtcgccac ggcgctcctc gtcccggtcg
     5461 ccctcgccct ctacctgccg ttcagcgtcc tcgtcgtcat gggacaggag tacctcccga
     5521 accacgtcgg gacggcctcg ggcgtgaccg tcggcctcgc ggtgacggcg ggcggcctgg
     5581 ccgcgccgct cctcggcctc gtcgccgacc gctggggcgt cgccggcccg ctcttcgtcg
     5641 tctccgcgct cccgatcctc gccgtcgcgc tcacgctgac gttgccggac tcgagggcgg
     5701 aggagacggt cgcccggggg tgaggggagg acggcgacgc cggaccgggt cgtcgtcccg
     5761 tgcgaaggcc gaacgcggac gagcgcgaag gagacccgtc ggccaccgca cgggacctag
     5821 aatcggcgtc gtgagcggac gagcggacag gagcggctgg cccgtgagac gggtcgcgct
     5881 cgcggacgag ggacgcggca tcgacccggc cgtcgccgcg atgacgccct cggagcggat
     5941 cgcgctcgtc gagcggctca cgaggaacgc ctgggccttc aagggagaac aggtcgatgg
     6001 aacgagactt cgtcgagatg ttgtccgcac tcgacgcggc cggggctgag tacctcgtgg
     6061 tcggggcgca cgccctggcc gcgcacggcg tgcctcgggc cacgggcgac ctcgacatct
     6121 gggtccgggc aacgcccgag aacgccgcga gggtgctggc cgcgcttcgc gacttcgggg
     6181 cgccgctctc cgacctgtcg ccggaggacc ttacacgccc cgagacggtc ttccagatcg
     6241 gcgtcgtgcc cgtccgcgtc gacatcctca cgagcatctc cggcgtcgag ttcgaagagg
     6301 cgtgggccgg ccggatccag gccaccgtcg acgccgtgac cttcggcgtc ctcggccgcg
     6361 aggagctgat ccgcaacaag cgcgcgacgg gacggccgaa ggacctgctg gacgtggaga
     6421 cgctgctcgg ggagccgggc gcccgctgac ccggtgtcga acgatggcga gattgtcctg
     6481 ggtctcgagt cgagggtcgg ggctcagcgc cgcttgtcgg tggagacctc tgccggcgcg
     6541 gcctcgccgt agtcgagcat ccagatgtcg ccctcgctgt tcgccgtcgt ccaatagacg
     6601 gatcggccgt cgggggagaa gcagaacgag ctgaagagcc cgtcgggagc tgttgagcca
     6661 gccggtgtga ccttccccgt cagccggtcg aggacctcca gcttcccgtc gcgagcgagg
     6721 aggatccggc gggagtcggg gagccacccg aggaggcgcc ccttcggcgg cgtggggatc
     6781 gactcgtagg tccccgtcgc gacatcgagc gcgaagtgca cctcgctcac cgtcctgccg
     6841 tccggagtac gcgatttccc gaggatccgc cgaccgtcgg gcgaggccca ccacgtgtcg
     6901 aagaggttcc cgtccttcag gaggggcaac ggcgtcagcc gacgctcctc gggaggagca
     6961 gagagatcga ccgtgtgcgg ccggacctcc tttccgacgt ccacgtagag cgttcgccca
     7021 tcgggagaga cgacgctcgg gaacgcgccg ccttccttcg cagcccggag ctcgcactgc
     7081 cgtccgctgc cgtcgggtcg gatgctccag acctcgttca acccgtccgg cgcgatgccg
     7141 aagtagatcc tggagccgtc gggtgcccac cgagggaagt ccttcgccgg ggcgtcgtcc
     7201 gtgaggcgcc tcgtctcgcc agtctggacc cggacgagga cgatgtcctt gcggccgccc
     7261 cgttcgagga agactgtcag gagccactct ccgttctgcg agagacccgt gacgcgcagg
     7321 tcccgcgggc cgctgacgag ctctcgcggc gacccgatga gcctgcccct cacggggtcg
     7381 aagtcggacc ggcggatcgt gtacgccccg atccaggtcc cgaagagaat ccgtcgtccg
     7441 tccggcgtcg aggacgaggt ctgcgagacc gtcgccgagg cgacgaccgg ggccgcttca
     7501 tccgtcacct ttcccgtcgt ctcgtcgacg cccaccctcc agaggttgac ctggccgccg
     7561 acggccgagt cgaacgtgat ccctgaacgg ctccagtcgg gacgtgggtg ccaggtcgac
     7621 gtgatcggta cggtcagcgg cgtcggatcg ccaccggatg cgggaatcgt cgagaggccc
     7681 gggcttccgg ccagcagcct tccggcaccg ccctggacga aggcgatgcg tatccctgag
     7741 ggagaccagg tcggcgttcg cccgtcgccg tcgtagacct tcgtcgtctg tcccgtggcc
     7801 acgtcgacga tccagagttc gctctggttg cccgcggtcg gtcccggcgt cgtcgtcgcg
     7861 aagacgatcc ggcctccgtc gggcgaccag gccgggtcgt atccgaagtc ggtcaggcgc
     7921 cggggcgact cgccgctcgc ccccatgagg aagatcccgc cgccgtcccg ctcggagcgg
     7981 aaggcgatcg tcgacccatc cggcgagaac gccggttgga ggtcgttccc cgggaagtcc
     8041 ttcgtcaggt tcgtcgggtt ctcgccgccg acacggcgaa ggaatatgtc ggagcccccg
     8101 gcacgacggg cgacatagac aaagctccct ccgtcgggag agacgctcgg tccactttcc
     8161 attccggcct cccaggtgag ctgaacgagc ttcggaattg gcttcggctc ggcggatcgc
     8221 ccacgtccgc ggacgagacc ggcctggaag gcgccccaga ccgcggcggc gatcgccacg
     8281 cagagtcctg tggccgcgag cgtcgtgcgc ttgagggagg acctcgtcgc gctcacgggc
     8341 tccgccgtcg cgccgcccgc tgtcccgctc ctctctccgc cccgttgccc ccagatggcg
     8401 aggtcgcgcg ccaggtcgcg ggtcgagtcc cagcgctcgg
//
LOCUS       DAOTEZ010000168         8225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_6150_length_8225_cov_4.230600, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000168 DAOTEZ010000000
VERSION     DAOTEZ010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8225
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6150_length_8225_cov_4.230600"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1328)
                     /locus_tag="P5164_10160"
     CDS             complement(<1..1328)
                     /locus_tag="P5164_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HRY44299.1"
                     /translation="MPDIGEQRRGLLASIDALENELRELRDRADRVAALLESARRHLQ
                     WLDSAAGQPPAATPLAEAGLDAAGKVALFRSLFRGREDVFPRLWVNAKTGKKGYAPAC
                     RNEWVRGVCDKPRVRCGECPAQAFVPVSDDVVLDHLQGRHVVGCYPLLLDETCWFVAV
                     DFDKAQWQADVTAFARTCTDLGLPVAVERSRSGNGAHAWFFFSAPVKAASARRMASYL
                     LTETMSRRHELAMDSYDRLFPNQDTMPRGGFGNLIALPLQRGARNDGNTVFLDPDLDP
                     WPDQWAFLAGLRRLSPGDVEAIAAEAQRNGLVTGVRTVEAEEDDAVLRPWRAQPRKSG
                     PRPIRGPRPEKVHAVLAQRLFVEKQGLDSSLLDQIKRLAAFQNPEFFKKQNLRLSTAL
                     TPRVISCAEELPRFVSLPRGCVEDLGGRLAAHTIPLDLDDPFDNDSVQRAL"
     gene            complement(1368..2288)
                     /locus_tag="P5164_10165"
     CDS             complement(1368..2288)
                     /locus_tag="P5164_10165"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRY44300.1"
                     /translation="MTAASKKDLAASVLARLLARARETGDDYQTLVSRYVTERFLYRL
                     GVSRVGDRFVLKGAVLLRVWSPQPYRATRDLDLLHRGDGSPDVIRAEIAEILAVNVEP
                     DGVAFDPDSIRTEPIRPEDEYAGLRATIRVRCGTYRSTLQVDIGVADAVWPPVTAGPV
                     PCLLGMPEPSVLAYSRDSVVAEKLEAIIVLGDRNSRIKDFFDLRHLAENFAFDRPTLT
                     EAVRRTLERRKTVLTDDEPFGLTEAYWDSPGRSTQIRAFSRRAGLDVDTETVRKIPAV
                     LHAFLNPVLEDVRRGRQTHGTWTPPGPWKP"
     gene            complement(2285..2875)
                     /locus_tag="P5164_10170"
     CDS             complement(2285..2875)
                     /locus_tag="P5164_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018866576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HRY44301.1"
                     /translation="MERSQLSTIASLGVFRPSDLEAVGIPRHRLYSLLSEGRIERLSR
                     GLYASRRHRFTENHALALVCRRVPHAVVCLLSALRFHGLTTQTSPTVWIAIPEKARRP
                     HLEGQRLSVCRFSAPALEEGVETHDVEGVRIRVTGAARTVVDCFRYRNKIGIDVAVEA
                     LKDFTRTRRSGASELARHARARGVSRIMQPYMDAIA"
     gene            complement(3224..3634)
                     /locus_tag="P5164_10175"
     CDS             complement(3224..3634)
                     /locus_tag="P5164_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44302.1"
                     /translation="MPIDPSVLGFANRWYPEALRTQRSVRLPSGVEIAVVSPPAFLAT
                     KLEAFLGRGRGDYASSHDLEDFVSVVDGRGGIVEEVSASSDDLRSFLSIELGRFLTDE
                     RFLSALPGHLPSDSGSQARVPILLRRLRALAGRA"
     gene            complement(3919..4431)
                     /locus_tag="P5164_10180"
     CDS             complement(3919..4431)
                     /locus_tag="P5164_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011050741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRY44303.1"
                     /translation="MALKPQDLLVALKLWVGRKQTWTYPLLARSLGISVSEAHGAVKR
                     AVAAGLLPAGGLGVRPRAESLREFLVHGAKYSFPALRGDLARGIPTAHAAPPLKELLA
                     DASEPPPVWPHPNGTARGPSLAPLFPSAPGAALEDPALYELLALFDALRAGRAREREM
                     ATRLLEERLR"
     gene            complement(4817..5125)
                     /locus_tag="P5164_10185"
     CDS             complement(4817..5125)
                     /locus_tag="P5164_10185"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRY44304.1"
                     /translation="MLEATERIERSVAGLDRTAFLADERTPGVVVRNLEILGESAPHP
                     PDDLEARTSDLQRPPIVGLRNRVVHGPLAVDLELVRRIVVNDLPGLVSRLRALRDSLD
                     "
     gene            5281..6831
                     /locus_tag="P5164_10190"
     CDS             5281..6831
                     /locus_tag="P5164_10190"
                     /inference="COORDINATES: protein motif:HMM:NF018684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HRY44305.1"
                     /translation="MPASRALVLRLVTIGLAAGAPALARGPESPGGEPRTHRFGDPAK
                     PSVALTFEEPGTPGAPCFLGLELLAGNGIEVRAATPTPTRATCRRTDSGWEVSGSSRL
                     DGRELAFRASVRADGSVLEWTSPRTVPLRDGSVHVLAGRYEGHGEPERAAAARASFEA
                     ADRELNAVYRRLVAARPPAVVAALRADQRRWLKYRDFTLHDGDDSAFVGPGTSAHLRQ
                     QALRTLERLRYLGALLDPPRAAFGETLYSDGLSGTIAVRRLGDAVAFAVRAGIPGFDL
                     PGVPRGVPLRVSGVARPAGPSAWEAPPESMTVAERTWAAERLRLSLGADGRLRVAAAG
                     GEAGLLARVLTGEYAPLRRLAPADSPLRALTSDLPARAFDDTTDGLAGAERQALLGTG
                     AGGSFRIGAESSDRLFLRFPGGHVGIALFVGADGSVVLAVEQVNGRNLSLRLWRQTAW
                     GEPFLAWTEGLPTLPANTVPHLDDSGTIRLARSGVGRGADSDAPHFLSWDGFGFAVER
                     AGTAGRAP"
     gene            6913..7152
                     /locus_tag="P5164_10195"
     CDS             6913..7152
                     /locus_tag="P5164_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HRY44306.1"
                     /translation="MSDPKVAAKQPVAVKVEAGRTYAWCRCGESKGQPFCDGSHKGTG
                     LVPLVWTADESKEVWLCQCKHTKKAPYCDGTHKSV"
     gene            7199..8086
                     /locus_tag="P5164_10200"
     CDS             7199..8086
                     /locus_tag="P5164_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRY44307.1"
                     /translation="MSGAGEPSSSFTIIDARVRDIGGFTVRRLLPWRARRMVGPFVYF
                     DHMGPVGLEAGRGVDVLPHPHIGLATVTYLYEGALLHRDSLGSEQVIRPGDVNWMTAG
                     RGIVHSERTPPAERAAGPRLHGLQLWVALPTADEEIEPSFRHHPGATLPEMERGGVRL
                     RLMAGSAWGATSPVEVRSPLFYVDVAMPAGSELPLTDEHAERAVYVVEGSLGAAGASA
                     GPGRMLVFAEGADVTLKAEAATRAVLVGGAPLDGTRNIDWNFVGSSKERIEKAKADWR
                     EGRFPKVPGDETELVPLPG"
BASE COUNT         1252 a         2784 c         2950 g         1239 t
ORIGIN      
        1 agcgcgcgct gcaccgagtc gttgtcgaag ggatcgtcga ggtcgagcgg gatcgtgtgt
       61 gcggcgagcc gtcccccgag atcctcgacg catccgcgcg gcagggacac gaagcgagga
      121 agctcctcgg cgcagctgat gacccgtggc gtgagggccg tcgagaggcg aaggttctgc
      181 ttcttgaaga actcgggatt ctgaaaggcc gccagtcgct tgatctggtc gaggagcgag
      241 gagtcgagac cctgcttctc gacgaagagc cgctgagcga ggacggcgtg gaccttctcg
      301 gggcgaggac ctcgaatggg tcgcggaccg gacttccggg gctgcgcacg ccacggacgg
      361 aggacggcgt cgtcctcctc ggcctcgacg gtccgaacgc ccgtgacgag cccgttccgc
      421 tgtgcctcgg ccgcgatcgc ctcgacgtcg ccgggcgata gtcgccggag accggcgagg
      481 aatgcccact ggtccggcca gggatcgagg tccgggtcga ggaagaccgt gttcccgtcg
      541 ttccgagcgc ctctctggag gggaagcgcg atcaggttgc cgaagccgcc tcggggcatc
      601 gtgtcctggt tggggaagag gcggtcgtag gaatccatgg cgagctcgtg ccgccgggac
      661 atcgtctcgg tgagcaggta cgaggccatc cttcgagccg aagccgcctt cacgggcgcg
      721 gagaagaaga accaggcatg cgccccgttc ccggagcgcg atctctcgac cgcgacgggc
      781 aggccgaggt cggtacaggt gcgagcgaag gccgtaacgt cggcctgcca ctgcgccttg
      841 tcgaagtcca cggccacgaa ccagcacgtc tcgtcgagga gcagcggata gcagccgacg
      901 acgtgccgcc cctggaggtg atccagcacg acgtcgtccg agacagggac gaacgcctgg
      961 gccgggcact caccacaccg tacccgtggc ttgtcgcaga ccccgcgcac ccactcgttg
     1021 cggcaggcgg gcgcgtagcc cttcttcccg gtcttcgcgt tcacccagag tcgcgggaag
     1081 acgtcctccc ttccacgaaa caggctccgg aagagtgcga ccttgccggc ggcgtcgagg
     1141 cccgcttcag ccagaggtgt ggcggctggg ggctggcctg cagccgaatc cagccactga
     1201 aggtgccgtc gggccgactc gaggagcgca gcgactcgat cggcccggtc tcgaagttcg
     1261 cggagctcgt tttcgagggc gtcgatcgag gcgaggagcc cccgccgctg ttccccgatg
     1321 tcgggcatct caggcagcgg gagcggcggt gggccgcggg gctcgcttca cggcttccag
     1381 ggtccgggcg gcgtccatgt cccgtgagtc tggcgaccgc ggcgcacgtc ctcgagaacg
     1441 ggatttagga aggcatggag aacggccggg atctttctca ccgtttcagt gtcgacgtcg
     1501 agccccgccc gcctcgaaaa cgcgcggatc tgggtcgacc ggcctgggga atcccaatag
     1561 gcctccgtca gcccgaaggg ctcatcatcg gtcagaaccg tcttcctgcg ctcgagcgtg
     1621 cgacggacgg cctccgtcag tgtcggtctg tcgaacgcga agttctctgc gaggtgcctg
     1681 aggtcgaaga agtccttgat ccggctgttg cgatcgccga ggacgatgat cgcctcgagc
     1741 ttctcggcga cgacggagtc gcgcgagtag gccaggacgc tcggttcggg catcccgagc
     1801 aggcatggca cggggccagc agtaaccggg ggccagacgg cgtcggcgac gccgatgtcg
     1861 acctggagag tcgatcggta cgtcccgcag cggacccgga tcgtggcccg aaggcctgcg
     1921 tactcgtcct ccgggcggat gggctcggtc cgaatcgagt ccgggtcgaa cgcgacgccg
     1981 tccggttcga cgttcaccgc gaggatctcc gcgatttcgg cgcgaatcac gtccgggctc
     2041 ccgtctcctc gatgcaggag gtcgagatcg cgggtggcgc ggtacggctg gggcgaccag
     2101 actcgaagaa ggacggcccc cttcaggacg aaccggtcgc cgactcggga gacgccgaga
     2161 cggtagagga agcgctccgt cacgtagcgc gagaccagtg tctggtagtc gtcgcccgtc
     2221 tctctcgcgc gggcgagcag ccgtgcaagg actgaggctg cgaggtcctt cttcgaggcg
     2281 gccgtcacgc gatggcgtcc atataaggct gcatgatccg gctgactccc cgggcgcgcg
     2341 catggcgggc gagctcgctt gcgccagacc ggcgcgtcct cgtgaagtcc ttgagggcct
     2401 cgacggcaac gtcgatgccg atcttgttcc ggtagcggaa gcaatcgaca acggtccgcg
     2461 cggcaccggt cacccggatc cggacgccct ccacgtcatg ggtttccacg ccttcctcga
     2521 gggccggcgc ggagaaccgg cagaccgaga gtcgctggcc ctcgaggtgg ggtcgccgtg
     2581 ccttttccgg gatcgcgatc caaacggttg gggaagtctg ggtcgtgagg ccgtggaagc
     2641 gcagtgccga caggagacag acaaccgcgt gcggcacacg gcgacagacg agggccagag
     2701 cgtggttttc ggtgaaacgg tgccgccggg aggcatacag cccacgggac agcctttcga
     2761 tcctcccctc ggagaggaga gagtagaggc gatgcctggg gatgccgacc gcttccaggt
     2821 ccgaaggacg gaagactccg agggatgcaa tggtcgagag ctgcgagcgt tccacgacac
     2881 cgagacgatg gtacaaaacg tcggggatcg caagcagaac ccgacgtttc gtctctgacc
     2941 cggacccgga gacgggcctg gcggcgcgct tgtcgaaagg cgaggaagtg tccacacttt
     3001 tgtccacacc ggccggaccc atcggtctct cgggggctcc cttgggatcc caggagctct
     3061 ttcggacgag gcgagcaacc cgcgtgacca tgaaagactt agtggccctc tggatcccgc
     3121 agtctccgtg cggcgtcttc cgggtccgga cgtcgaaaac gctccttttc cccatagggt
     3181 tcaagggtcg cgggttcggg acgccgtcag ccgaactcgt tgactatgcc cttccggcga
     3241 gagcgcgaag tcgccgaagc aggatcggca cccttgcctg gcttccgcta tccgacggca
     3301 gatgacccgg cagtgccgag aggaaacgct cgtcggtcaa gaacctcccg agctcgatgc
     3361 tgagaaacga gcgaaggtca tcggacgagg ccgagacctc ctccacgatc ccgccgcgac
     3421 cgtcgacgac gctgacgaag tcctcgaggt cgtggctgga ggcgtagtcg cctcgccccc
     3481 ttcccaggaa cgcctccagc ttggtggcca ggaaggccgg cggtgagacc accgcgatct
     3541 cgacgccgct cggaagccgg acgcttcgct gcgttcgcag cgcttccggg taccagcggt
     3601 tggcgaagcc gagcaccgaa ggatcgatcg gcatgacgtc gaggagagtg tcgccgaagg
     3661 tccatcggca gatcggagcc cccgcgctct gatcgtggcg gaatccgcgc tccaacatcg
     3721 acttctcgag cgccgagtac gcggaccggt ctggtgtctc gacgatcacg tcgacgtccc
     3781 gcgtcgcccg gatcggcgca gcggcaggat ccgtgatcag gagtcccgtg gcacaccccc
     3841 cgaggaagac catcccgtga cgcaggggcc cgagggcttc ggcgatgcgc tcgagcatgg
     3901 cgaggttggg gtccggggtc acctcagccg ctcctcgagg agtcgcgtgg ccatctcgcg
     3961 ctcgcgagca cgtcctgccc gcagggcgtc gaaaagagcg aggagctcgt acaaagcggg
     4021 atcttccagc gcggcgcccg gtgcggaagg aaagagcggg gcgaggctcg gtccacgcgc
     4081 ggtgccgttc gggtgaggcc agacgggagg aggttcggaa gcgtccgcga ggagttcctt
     4141 cagcggcggg gcggcgtgcg cggtcgggat tcccctggcg agatcgcccc gaagcgccgg
     4201 gaacgagtac ttcgctccgt ggacgagaaa ctcccggagt gactcggcgc ggggtctgac
     4261 gccgagccct cctgctggca gcaggccggc cgcgaccgcc cgcttcaccg cgccgtgagc
     4321 ctccgacacg ctgatcccca gggatcgtgc gaggagcggg taggtccagg tctgcttgcg
     4381 tccgacccag agcttgagag cgacgaggag atcctgcggc ttcagggcca tacagggagt
     4441 gtattcgtga ttcgcgaaac gcgaaacgat cgattttgat cttgttctgt cggcccgtcg
     4501 tgtggccgta gaggggggta aggcgaagag gtggccacac tgatgtccac gtcgaccgta
     4561 cccatcaggc tccaggagtc accgtcgcgg cccagggagc gccgcaccgc gaggcgaaca
     4621 acctcttcac gatgagtggc ttagactccc accggatccc gtggtctccc cttgggatcc
     4681 cagggctccc ggcgtcgcaa gtgctctttc cgcccatagg gttcaagggt cgcgggttcg
     4741 aatcccgccg tcccgaccat tgattccgaa cgatttggcg ccctttgggg gcgctctctc
     4801 gtgcgccgtc gaagagtcag tcgagggaat cccggagcgc gcgaagtcgc gacacgaggc
     4861 cgggcaggtc gttgacgacg atcctccgga cgagctcgag gtccaccgcc aacggaccgt
     4921 ggacgacccg gttcctgagg ccgacgatcg ggggccgttg caggtccgaa gtgcgcgcct
     4981 cgaggtcatc cggcgggtga ggggcggact ccccgaggat ctcgaggttc ctcacgacga
     5041 cgccgggggt cctctcgtcg gcgaggaacg cggtccggtc cagccccgcg acggagcgct
     5101 cgatccgctc cgtcgcctcg agcatgtctt cgaggaggag gcggacggat cacttcgaca
     5161 tgaggcgcct cgggccggat ggcgtccatg tgccgcggtc ggagcgaagt gtggagatca
     5221 ggtcgcccgg cctgccggct cgcatcgccc tagaatccca aagcggggga gaggcccgaa
     5281 atgccagcgt cgcgcgcact cgtccttcgc ctcgtcacga tcggtctcgc cgccggggct
     5341 ccggctctcg cgcggggacc ggagagcccg ggcggagagc cgcggacgca ccgcttcggc
     5401 gaccccgcga aaccttccgt cgcgctgacc ttcgaggagc ccggaacgcc cggggcgcct
     5461 tgcttcctcg ggctcgagct gctcgcgggc aacggcatcg aggttcgcgc ggccacgccg
     5521 acgccgacgc gggcgacctg tcgccggacg gactcgggct gggaggtctc cggatcgagt
     5581 cgtctggacg ggcgggagct cgccttccgc gcgagcgtcc gcgccgatgg gagcgtcctc
     5641 gaatggacgt cgccccgtac ggtgccgttg cgcgacggga gcgtccacgt gctcgccggc
     5701 cgttacgaag gccacggcga gccggagcgg gctgccgccg cccgcgcgtc gttcgaggcc
     5761 gccgatcggg agctgaatgc cgtatatcga cgtctcgtgg cggcgcggcc gccggcggtc
     5821 gtcgctgccc tgcgtgccga ccagcggcgc tggctgaagt accgcgactt cactctccac
     5881 gacggcgacg actccgcgtt cgtgggaccg gggacgtcgg cccacctgcg tcagcaggcg
     5941 ctgcggacgc tcgagcggct ccgctacctg ggcgcgctcc tcgacccgcc gcgcgccgcc
     6001 ttcggtgaga cgctctactc cgacggcctg agcgggacga tcgccgtccg tcgcctcggc
     6061 gacgccgtcg ccttcgcggt gcgcgccggc attcccggct tcgatcttcc cggcgtcccc
     6121 cgcggcgtcc cgctccgcgt cagcggcgtc gcgcgccccg cgggcccgtc cgcgtgggag
     6181 gcgccgcccg agtcgatgac ggtcgcagaa cggacctggg ctgccgagcg gcttcggttg
     6241 tcgctcggcg cggacggccg cctgcgcgtc gcggccgccg gcggagaggc cggcctgctg
     6301 gctcgcgtcc tcaccggcga gtacgcgccg ctgcgccgcc tcgcgcccgc cgactctccg
     6361 ctccgtgcgc tcacctcgga tcttccggca cgggccttcg acgacacgac ggacgggctc
     6421 gcaggagccg agcggcaggc cctgctcggc acgggagcgg gcggctcgtt ccggatcggg
     6481 gccgagtcgt ccgaccggct cttcctccgg ttcccgggag gtcacgtcgg gatcgccctc
     6541 ttcgtgggcg ccgacggcag cgtcgtcctc gcggtcgagc aggtcaacgg gcgaaacctc
     6601 tccctgcggc tctggcggca gaccgcgtgg ggcgagccgt tcttggcgtg gacggagggc
     6661 ctgccgacgc tcccggcgaa caccgtgccc cacctcgacg attccgggac gatccggctg
     6721 gcgcggtccg gagtcgggcg cggggcggat tcggacgctc cccatttcct ttcgtgggac
     6781 gggttcggct tcgcggtgga gcgcgccggt actgcggggc gcgcaccctg attccgtccc
     6841 ggaagcccga atcggtcttt gaacggcgag tcccagggac taccgtacgg tccggtcgaa
     6901 gaggaggaaa cgatgtccga tccgaaggtc gccgcgaagc agcccgtggc cgtgaaggtg
     6961 gaagcgggcc ggacgtacgc ctggtgccgc tgcggagagt cgaaggggca gccgttctgc
     7021 gacggctccc acaagggaac cggcctcgtg ccgctcgtct ggacggcgga cgagagcaag
     7081 gaagtctggc tctgccagtg caagcacacg aagaaggcgc cctactgcga cgggacccac
     7141 aagtcggtct gagcggtccg agcgtggcgg ggaggggtcg gaccgggagg ggcgggcgat
     7201 gagcggggct ggggaaccgt cttcgtcctt cacgatcatc gacgcgcgcg tgcgcgacat
     7261 cggcggcttc accgtccgcc gcctcctccc gtggcgggcc cgccgcatgg tcgggccgtt
     7321 cgtctacttc gaccacatgg gccccgtggg gctcgaggcc gggcgcggcg tcgacgtcct
     7381 cccgcacccg cacatcggcc tcgccacggt gacgtacctc tacgagggag cgctcctcca
     7441 ccgcgacagc ctcggcagcg agcaggtcat ccgcccgggc gacgtcaact ggatgaccgc
     7501 gggccgcggc atcgtccact ccgagcggac gccccccgcg gagcgggccg ccgggccgcg
     7561 cctccacggc ctccagctct gggtcgccct ccccacggcc gacgaggaga tcgagccctc
     7621 gttccgccac cacccgggcg cgacgctgcc cgagatggag cgcggcggcg tgaggctgcg
     7681 cctgatggcc ggctccgcct ggggcgccac gtctcccgtc gaggtccgct cgccgctctt
     7741 ctacgtcgac gtcgcgatgc cggccggctc cgagctcccc ctcacggacg agcacgccga
     7801 gcgggccgtc tacgtcgtcg agggctcgct cggcgccgct ggcgcgagcg ccgggcccgg
     7861 ccggatgctc gtcttcgcgg agggcgccga cgtcaccctg aaggcggagg cggcgacgcg
     7921 ggccgtcctc gtcggcgggg cccctctcga cggcacgcgg aacatcgact ggaacttcgt
     7981 cggaagctcg aaggagcgga tcgagaaggc gaaggcggac tggagagagg gccgcttccc
     8041 gaaggtgccg ggcgacgaga cggagctcgt cccgctgccg gggtgaccct ccacgatcaa
     8101 acctcacccc cgccccggca gctccacccc gttcttcgcg aagacgtgca gccgcagccg
     8161 cgcgcgggag gccgcgacgt agaggttgcg gggcttcatc gcccactcgc tcccgtccac
     8221 gtcga
//
LOCUS       DAOTEZ010000169         8209 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_6165_length_8209_cov_5.687270, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000169 DAOTEZ010000000
VERSION     DAOTEZ010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8209
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6165_length_8209_cov_5.687270"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="P5164_10205"
     CDS             complement(<1..417)
                     /locus_tag="P5164_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003104711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRY44308.1"
                     /translation="MAAGAERPGRREERACRAESPLLVAEDAATRELFETVRKVAATR
                     LSVLVLGESGTGKEVVAREIHRASGRPGPFVAVNVAAFAESLAEAELFGAARGAYTGA
                     ERDRAGVLEASSGGTLFLDEIGDLSPRIQAKLLRVLQ"
     gene            complement(3438..4691)
                     /gene="aroA"
                     /locus_tag="P5164_10210"
     CDS             complement(3438..4691)
                     /gene="aroA"
                     /locus_tag="P5164_10210"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRY44309.1"
                     /translation="MITLPSGRLAGRLRVPGSKSLTNRALVAASLAAGRSELVCPLDC
                     DDTRVLAEALGRLGAEVRRGDRSWIVEGPLTGAPGRERTIDVGPAGTPARLLLALTAA
                     LPGRFVLDGSPRMKERPMGPLVEALASRGARIEPLGREGFLPLRIEGGTLGAGSVSIR
                     SDVSSQFVSALLLASPAVAGGLDVRTEGRAASGSYVELTRQVLAVFAPEGRPRPARYV
                     VPADDSAACFPIAGALVSRGRVELEGLDPDSAQPDAVFRRWAASAGGALRWEGSGEGA
                     VLVVEGAARRGLDADVDPAPDAALPLAALVAFAGGASRLRGVARLREKESDRLAAAED
                     LLARAGAEARVEAGPGEEGLLVDGPRGRPVRADFRAWGDHRVAMAAAVLALSLPEGST
                     LDDPGVVSKSYPSFWSDWRSLVSTR"
     gene            complement(4688..5470)
                     /locus_tag="P5164_10215"
     CDS             complement(4688..5470)
                     /locus_tag="P5164_10215"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRY44310.1"
                     /translation="MVPYPEPRNVAEGGVLVFADAHLGQAEGDAGDFLSALEEGVARG
                     FRTVVLLGDIFHYFIGSEKLETPLIREVLEGWDDLASRGVDLRYVEGNRDFFLRGTRW
                     ARAFAAYGDTDGVEVGGRRYAFVHGDRVNTRDLPYRFWRVVSKNPVSRGALALIPGPL
                     AREIVRRTEARLYTTNFRHKRVLPGAELVREGERARAAGYDELLVGHFHVEQHYERAG
                     SVTRVLPAWLEERRHGEIGSDGVLRIVTEASAHRAVRRAPPT"
     gene            5569..>8209
                     /locus_tag="P5164_10220"
     CDS             5569..>8209
                     /locus_tag="P5164_10220"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRY44311.1"
                     /translation="MKSVRALGALLAAAALAPASLPAAAGTAKLHVGEGVQDRVAAEG
                     RTRVLVARDAVPDSPVPEGLEVVRSLDGGRILAGWLNATGLERLGREPGARAVVLDRV
                     VRPAGQVGTAQIGADRLFSAGLTGTGRAIGIVDTGVDSFHPDFGAGPSGPGRILGGWN
                     FADGNDKTQDCNGHGTAVAGVAAGHQGIAPGASIVPLKVFGNRDGCSAALSSDVLAAV
                     DWALGHREALGLEVLNLSLADERVRSGFCDAEDPVSAGLFARARAEGLVVVAASGNAG
                     FPSAISWPACHSDVVSVGMVYAAGQGPHAWEGDAECIDPYTGPDVVPCTSNGGPALSL
                     LAPGVRWIAPTSGGGRFTTFSGTSAAAPAAAGALLLTRQFTSFDDPALSTDFLRLTGV
                     PVGDGRTALATPRLDVGTALVAPSPASGPCENLEASGARPAAMVCRATVSAQTGNVSS
                     LAVALSLEHPRLATVRATLTGPDGTTAHLVDGIRREGTVFREVFGRTVESSEPLSLFA
                     GRPVAGTWVLRLEDDGGFRDGRVTSWALVAEPEAPRAVRLAEAPSRFLPSVARNTGRF
                     GSFFSSDVVLFNPHLEPADVTLSFLPSGAAPDQAVAVDLRLGARATRTLSDVVGNAFR
                     SSGFGPVRIDAPAHVMVVSRTRTTAPGGGTYGLFVPPLARDAAAGVPDPPTYLAPVFH
                     AERSRVNVGLAEVSGGEANVEILVRDGRGSVKARFGVTLGPFASRQVSDVHAAASTVP
                     ADGDLFEVRVLSGSGYVASWAIAVDNGSNDGLLVAASRPRRDAFLPAAARSPGRFGAY
                     FRTDLKLSNPWTSPARVRISFFPSRGAASKELVLSLGAFETRLFEDVLGSLLGLPEDT
                     AGALRITALGDGPGLIASSRTYTE"
BASE COUNT         1037 a         3222 c         2959 g          991 t
ORIGIN      
        1 ctgcaggacc cgcaggagct tggcctggat gcgcggggag aggtcgccga tctcgtcgag
       61 aaagagagtc ccccccgagg aggcttccag cacgcccgcg cggtcgcgct cggccccggt
      121 gtaggcgccc cgcgcggccc cgaacagctc ggcctccgcg agcgattccg cgaaggcggc
      181 gacgttcacc gcgacaaagg ggccgggccg gcccgaggcg cggtggatct cccgcgcgac
      241 gacctccttg cccgtcccgc tctcccccag gacgaggacg gagagtcgcg tcgccgcgac
      301 cttccgcacc gtctcgaaga gctcccgggt cgccgcgtcc tccgccacga ggagcgggct
      361 ctcggcgcgg cacgctcgct cctcgcggcg cccggggcgc tcggctccgg cggccaggcg
      421 cgccgcggcc cgctccagcc ccgccctgcg ggcggaccgt tccccgaggt cccggagcgc
      481 ggcgccgtcg gtgccggcga acgcttccgg gaaccggccc cgccactcga ggagacgggt
      541 cgccaccgcg agcggaccgt cgggcgattc gagccgcgtc gcgaggacgc gccaggcggc
      601 tgccggagga ggcaggccgc ggccgagcct ctcctcggcg tcgaggagcg tcgtctcgtc
      661 cccgtccagc cccgcgggag gtctcccggg gcggccgagg tcgcggagcg ccgacgcggc
      721 gagacggcgc cggaggggct cgacgccgct ccgggcgtcc ggatgaggcg cggcctcctc
      781 gagccgcgcc agcgcctcgg ccgcggagcc cgcggcgagg agccgggccc cctcgaggac
      841 gaggacttcg cgccgggccg ggtgctccgg ccgctcccgg agcgccaaca caccctcggc
      901 gaaggcttcg gccccttcgg catcgtctcc gtccgcgagc gcgaggcgct gccggagcgg
      961 gatcgagaag aggagctgat ggccgcgtcc cggccgccgg tcgtgcgcga ggacgacctc
     1021 gaggtcccgg tacgccgagc ctgcgtcgct ccttccgagc gcgagggcgg cgcgctggcc
     1081 gagcgccgag acgtagcgct ccctctcgcc gagcgaagag aaaagcgaaa gagcctcctc
     1141 gagcgcccgc tcggcgtcgg cgcgcgctcc ttccatcgcc gccgcgaccg agaggtcgaa
     1201 gagggcgtcc gccctccgcg ccggctcgcg cgcctcgtcg agcgccgccc ggaagaaggt
     1261 gcgcgcctcg cggggccggc cgagcccgag agcgaggtag ccggccgtga accgcgcgtc
     1321 gagcaggccc ggctcgttcg acgggccgtc gagggcctgc gccgcggaga ggagctccgc
     1381 ctcggccctt tcgagctcct gccgcctctc gtgaagctcc gcccgaagga ggcagccgcg
     1441 gactcctccc gcggcggcgc cgatcgcgtc cagcatccga ccggcctccg cctcccgccg
     1501 caggtccagg aggagggacg cgcgggcgag ccgcgtctcc gtgtcgccgt cgtcgcccac
     1561 gagccccagc gcggtctcgg cctggcccat ccggacgagg agccgcgccg ccgagacgcg
     1621 ccgggagagg ggctcggacg ccgccgtgcg agggtcgatc cgctccccga cgctccgcgc
     1681 ctcttccatc ctccccgcgg ccgcgtacac ctcgagcgcg aggagcggcg ctccgaggtc
     1741 gggatcggac gccggcgccc gggacaggag cgcgagcgcc tcctccgccg ccccctcccg
     1801 cgacgcctcc aggaagcggg aacgggccgc tgcgacgacg tcagggcgtt ccccgaacgc
     1861 ggcgagccgc gccagacgga gcctcccgtc gtctcggcca gcggcctcga gcctcgccat
     1921 ccccgcttcc ttctccgcgg gacggagaga gccggcaagc cggcggcgtt gagccggatc
     1981 ggcgaaccga tagaggccgt ccgccgtccg cgcgagcgct ccctcgtcca tcgcttcgtc
     2041 gaggacctcc gcgacgcgcg acgcgccgac gccggaaacg gcggccgcct cctccgtcga
     2101 aaagccgtct ccgaggagcg cgctcgcctt gaggacgatc tccgcctcgg gatgcgacgt
     2161 gcgagccgtc gcggagcggc ccgcccccgg agcgaggaag gcgcgcgcct tcctccgcgc
     2221 cgcgcgcagg aggcgtcccg cgtcgccggc ggcctcctcg gcgagctcgg tccagagacg
     2281 gaccgcggcc gaaggcgagg cgtagggcag gtggacgagc ccggcgacct cttcccggcc
     2341 gagcctcgga aagacgaggc tcgccttctg ccacgggggc ggcgcgtccg accgcgtctc
     2401 gatccgtatg acgcccggca ggacccgcgg gatcgcctcc cacgcctcga cggcgcccgc
     2461 tccccacgcg tcgagcccga gaccgagcag gacgagcggc gggccctcga ccgacgcgac
     2521 cggctccccg cgcgcgagcg cccggaggat gtcggcgcgc gggtcgggcc cgagccccgt
     2581 cgcgagcgcg aggatcggag ccccctcctc gagagagccc gggggccccg tctccgagaa
     2641 cgcgacttcg tccggcgcac gacgggcggc gccgcgcgcc acccagtcgg ccgcggatcc
     2701 ggtcgagacg agccgctcgg cggaatcccc gtccagctcc cggggcagga cgagtcccag
     2761 gaggtcgagg gcgaccttcg cggacccgcc gcacgggcgg gcgaggtcga ggaggagcgc
     2821 gtcggagggg cgcgcgctcg catccgaccg gagcacggtg gcgaccacct cggccgtccg
     2881 agccgggagg ccgtccgaga gcgcctccgc gagcgattgc ctcacgtggc cccgccggtc
     2941 gaagcgaggc tctcgtcccg cgaggcgcag ggcgacgccg cccaggaccg agtggaccgg
     3001 agcggcccgc caggccggga cgggcggcga caggagccag gggacgtcgc gcgtcgaagg
     3061 cttcgcgccg agcgcgagcg cgccgtccgc cgagagcccg agcccatggg ccgcgaggaa
     3121 cgacaggagg cccgcgaggc ggacgaggaa ggcggagtgc tcggcgtccg agaggtccgc
     3181 gggaagcggg cgcggcgccg gcaccggacc cgcgacgaag acgccggccg cggtgcgccc
     3241 ccacgcgagc tccgggagcg agggatggac gagccgcgcc acctcggcgg cccccgcggc
     3301 gacgtccgcc gccgggatca cccattccgg cggccgcgcc gcacctctct ccgcgcgccc
     3361 tctgccccgc actcctcctc cggacgaacg cggaatcttc gcacggtccg gcgggccgta
     3421 ccttcgcgcg cggcgcctca gcgggtcgac acgagcgagc gccagtcgct ccagaaggag
     3481 ggatacgact tcgagacgac tcccgggtcg tcgagcgtcg acccctccgg cagcgagagc
     3541 gcgaggacgg ccgcggccat cgcgacgcga tggtcgcccc acgcgcggaa gtcggcgcgg
     3601 accggcctcc cccgcggacc gtcgacgagc aagccctcct cgccgggtcc cgcctcgacg
     3661 cgagcctcgg caccggcccg agcgaggagg tcttcggcgg ccgccagccg atccgactcc
     3721 ttctccctca gcctcgcgac gccccggagg cgcgaggctc cccccgcgaa tgcgacgagc
     3781 gccgcgagcg gaagcgcggc gtcaggcgcc gggtcgacgt cggcgtccag gccgcgccgc
     3841 gccgcgccct cgacgaccag tacggcgcct tcgcccgacc cttcccagcg gagcgcccct
     3901 cctgcggaag ccgcccatcg tcggaagacg gcgtcgggct gcgccgaatc cggatcgagg
     3961 ccttcgagct ccacccggcc gcgcgagacg agagccccgg cgatcgggaa gcaggcggcc
     4021 gagtcgtccg ccgggacgac gtagcgggcg ggccggggac gcccctcggg cgcgaacacg
     4081 gcgaggacct gccgggtcag ctcgacgtag gaccccgagg cggcccgccc ctccgtcctc
     4141 acgtcgagcc cgccggcgac cgccggcgag gcgaggagga gcgcggagac gaactgcgag
     4201 gagacgtccg accggatcga cacgcttccc gccccgagtg tccccccctc gatccgaagc
     4261 gggaggaatc cctcgcggcc gagcggctcg atccgggcgc cgcgggaggc cagcgcctcg
     4321 acgagcggtc ccatcggcct ctccttcatc cgggggctcc cgtcgaggac gaacctcccg
     4381 ggcagcgctg ccgtcagcgc gaggagcaac cgggccggcg tgccggccgg accgacgtcg
     4441 atcgtccgtt cgcggccggg cgccccggtg aggggaccct cgacgatcca gctccggtcg
     4501 cctctccgga cctccgcacc gagccgtccg agcgcctccg cgaggacccg cgtgtcgtcg
     4561 cagtcgagag ggcagaccag ctcgctcctc ccggccgcga gggaggcggc gacgagggcc
     4621 cggttcgtga ggctcttgga gccggggacc cggaggcgcc ccgcgagcct tccggaaggg
     4681 agcgtaatca cgttggaggc gctcggcgga cggcgcgatg cgcgctcgcc tccgtcacga
     4741 tccgaaggac gccgtcggat ccgatctccc cgtgccggcg ctcctcgagc caggctggca
     4801 ggacccgcgt cacgctgccg gcccgctcgt agtgctgctc gacgtggaag tgcccgacga
     4861 gcagctcgtc gtatccggcg gcccgcgccc gctctccctc gcggacgagc tcggcgccgg
     4921 ggaggaccct cttgtgccgg aagttcgtcg tgtagaggcg ggcctcggtc ctccgcacga
     4981 tctcccgggc gagcggcccg ggtatcagtg cgagggcgcc gcgcgagacg gggttcttcg
     5041 aaacgactcg ccagaaccgg tacgggagat cgcgcgtgtt gacgcggtcg ccgtgaacga
     5101 aggcgtaccg gcgaccgccg acctcgaccc cgtccgtgtc gccgtaggcg gcgaacgccc
     5161 gcgcccagcg cgtcccgcga aggaagaagt cgcggttccc ttcgacgtac cggaggtcca
     5221 cgcctctcga ggcgagatcg tcccagccct cgaggacctc gcggatcagc ggtgtctcga
     5281 gcttctcgct cccgatgaaa tagtgaaaga tgtcgccgag gaggacgacc gtccggaacc
     5341 cccgggcgac cccttcctct agcgcggaga ggaaatctcc tgcatccccc tccgcctggc
     5401 cgagatgagc gtcggcgaag acgaggacac caccttccgc gacgttccgc ggctccggat
     5461 acgggaccac gatgggattc tagcggcccg ggacactccc tcccgtcccg cgtgttgacg
     5521 cgtctcacgc cccggatcgg ccccgggtga tagcttcccc gctcgaaaat gaagtccgtc
     5581 cgcgccctcg gcgcgctcct cgcggccgcg gccctcgccc cggcctccct tccggccgca
     5641 gccggaacgg cgaagctcca cgtcggcgaa ggagtgcagg atcgggtcgc ggccgagggt
     5701 cggactcgcg tcctcgtggc ccgcgacgcg gttcccgact cccccgttcc cgaggggctg
     5761 gaggtcgtcc ggtcgctcga cggcggccgc atcctcgccg gctggctgaa cgcgaccggg
     5821 ctcgagaggc tgggccggga gcccggcgcg cgggccgtcg tcctcgaccg cgtcgtccgt
     5881 cctgcggggc aggtcggaac ggcccagatc ggtgccgatc ggctcttctc ggccggcctg
     5941 acgggaaccg ggcgtgccat cgggatcgtc gacaccggcg tcgacagctt ccatcccgac
     6001 ttcggcgcgg ggccgtccgg tcccgggaga atcctcggcg gctggaactt cgccgacggc
     6061 aacgacaaga cccaggactg caacgggcac ggcaccgccg tcgccggcgt cgccgccggg
     6121 caccagggga tcgcccccgg agcgtcgatc gtccctctga aggtcttcgg caaccgcgac
     6181 ggctgctccg ccgcgctctc ctcggacgtc ctcgcggccg tcgactgggc gctcgggcac
     6241 cgcgaagccc tgggcctcga ggtcctcaac ctgagcctcg ccgacgaacg ggtccgatcc
     6301 ggtttctgcg acgccgagga ccccgtttcg gccggcctct tcgcccgggc gcgcgccgag
     6361 gggctcgtgg tggtggccgc gtccggcaac gccggcttcc cgtcggcgat ctcctggccc
     6421 gcctgccatt ccgacgtcgt cagcgtcggg atggtctacg cggcggggca gggtccgcac
     6481 gcctgggaag gcgacgccga gtgcatcgac ccgtacaccg ggcccgacgt cgtcccctgc
     6541 acgagcaacg gcggtcccgc tctctcgctc ctcgctcccg gagtccgctg gatcgcgccg
     6601 acgtccggcg gcggccggtt caccaccttt tccgggacct cggccgcagc gcccgcagcc
     6661 gccggggccc tcctcctgac acggcagttc acctccttcg acgatcccgc gctctcgacc
     6721 gatttcctcc ggctcaccgg cgtgccggtc ggcgacgggc ggaccgcgct cgcaacgccc
     6781 cggctcgacg tcgggaccgc cctcgtcgcg ccgagccccg cctccggtcc gtgcgagaac
     6841 ctcgaggcct cgggcgcgag gccggccgcg atggtctgcc gcgccaccgt ctccgcccag
     6901 acggggaacg tctcttcgct cgcggtcgcg ctctcgctcg agcacccgcg gctcgcgacg
     6961 gtgcgcgcga cgctcaccgg gcccgacggc acgacggcgc acctcgtcga cggtatccgc
     7021 agggagggga ccgtcttccg agaggtcttc ggaaggacgg tcgagagctc ggagccgctc
     7081 tcgctgttcg ccgggaggcc cgtcgccggc acctgggtcc tccgtctcga ggacgacggg
     7141 ggattccgcg acggccgggt cacgagctgg gcgctcgtcg cggagcccga agcgccgcgc
     7201 gcggtccgtc tcgcggaggc gccgagccgg ttcctgccgt cggtcgcccg caacaccgga
     7261 cgtttcggct cgttcttctc gagcgacgtc gtcctcttca acccccacct cgagcccgcc
     7321 gacgtcacgc tttcgttcct gccgtcgggc gccgctccgg accaggcggt cgccgtcgac
     7381 cttcggctcg gcgcccgcgc cacgcgcacg ctgtccgacg tcgtcggcaa cgcgttccgc
     7441 tcgtccggct tcggccccgt ccggatcgac gctccggccc acgtgatggt cgtgagccgg
     7501 acgaggacga cggccccggg cggtgggacc tacggcctgt tcgtcccccc gctcgcgcgg
     7561 gacgcggccg ccggcgttcc cgacccgccc acctacctcg cgcccgtctt ccacgccgaa
     7621 cgctcgcgcg tcaacgtcgg tctcgccgag gtctcgggcg gcgaggcgaa cgtcgagatc
     7681 ctggtccgcg acggccgcgg ctccgtgaag gcgcggttcg gcgtgacgct cggtccgttc
     7741 gcgagccgtc aggtgagcga cgtccacgcc gcggcctcga ccgtgcccgc cgacggcgac
     7801 ctcttcgagg tgcgcgtcct gtcgggatcg ggatacgtcg cgagctgggc gatcgcggtc
     7861 gacaacggct cgaacgacgg cctcctcgtc gccgcctccc gcccgaggcg ggacgcgttc
     7921 ctccccgcgg cggctcgctc gcccgggagg ttcggggcct atttccgaac cgatctcaag
     7981 ctctcgaacc cgtggacctc gccggcgaga gtccgcatct ccttcttccc gtcgcgcggc
     8041 gccgcctcga aggagctcgt cctgtcgctc ggcgccttcg agacgcgcct gttcgaggac
     8101 gtcctcggga gcctcctcgg cctcccggag gacaccgcgg gcgccctccg gatcacggcg
     8161 ctcggagacg gtcccggact gatcgcttcc agccgcacct acaccgagg
//
LOCUS       DAOTEZ010000170         8200 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_6177_length_8200_cov_6.722406, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000170 DAOTEZ010000000
VERSION     DAOTEZ010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8200
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6177_length_8200_cov_6.722406"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /gene="mce"
                     /locus_tag="P5164_10225"
     CDS             <1..395
                     /gene="mce"
                     /locus_tag="P5164_10225"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HRY44312.1"
                     /translation="SHLGLAVPSVDGGGAIWDLLGLTEKHREEVASQKVITSFRPVGE
                     SSLELLEPTAPESPIAKFLEKKGPGIHHLCLEVPDVAATLEKLKAAGIRLINETPVPG
                     AHGCLVAFIHPASTGGVLLELSQPAPGH"
     gene            complement(453..2552)
                     /locus_tag="P5164_10230"
     CDS             complement(453..2552)
                     /locus_tag="P5164_10230"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HRY44313.1"
                     /translation="MTSFPARALALALLALPCSLHGAEAEPATSSLFRAVDDLSVPDS
                     SRPVKDLVLSAGAATIRLPEGRAAVVTDPDGVPAGLFLSGKGTFTYVPRDPREHSAVR
                     WTLSKNTRLSPTATEAGLAIADSFETLLWLAPAQTLPELPSPATEPSLGPAFRKHRDR
                     TAKVKEPPSAHLRAAQRRDAPGARVVRAETTGGREDLVFLHDPLESGRERITASGRFQ
                     VRVSRGDDRSYAILLAEQPIEGDLFDPPSPRVVLTHVDLKVTASGGSDVSISATETFE
                     ARRGAVGTLLLDLLDTAESQTGATIQQTALFGNVRRGSDRIRERPVTVTGVFDEAGNA
                     LEFDHRNDQLAVALRAPIAEGKSARVRVEVEGGLLVRPLGNNYWVLGLAPWFPAPPMA
                     GRAFSAQVTMSVPKPFHPIAPGVTLRRAEEGERNVLETRLDGPAQNLAVLAGAYSLEE
                     EVKDGRTLRVASYGGRNRTASRSLLDLGFATASFFEIFLGKMPVRELTIVEMAVWAQG
                     QAPAGLLLIGTEAFNPMGKGLYRRAAAGVNERFAHELSHQWWGNGVRLPDLSEQWLSE
                     SFAEYCSAFFIRQAAGKEAYDLYLTRWRSRAAQTKETVPLLLAHRLRAKNDQVQADNL
                     RTWLLYDRGPLVLAAIHREIGDEAFLTFLSSVQSTLGGRLGTTKRVEEVLEAVTRKDW
                     TPFFDRFVRGSEIPTVP"
     gene            complement(2585..2800)
                     /locus_tag="P5164_10235"
     CDS             complement(2585..2800)
                     /locus_tag="P5164_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44314.1"
                     /translation="MAELVWQLRKGPDGAPYAFAAFNRAEKRTNFFDGAEPLRAFLIK
                     HGVAADDAVALAARVEGGETVSVTLPK"
     gene            complement(2814..3146)
                     /locus_tag="P5164_10240"
     CDS             complement(2814..3146)
                     /locus_tag="P5164_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44315.1"
                     /translation="MDAGTLVLVHLHSPKEKYWGALRALTPAGVTVRGIDVALFDDWA
                     RQFLSGGEPELGVATLFFPLHRVEKMFEDSRVGTVAAYHERFLEMVGRDVRDVLRLDS
                     SRPDGPLQ"
     gene            complement(3212..3991)
                     /locus_tag="P5164_10245"
     CDS             complement(3212..3991)
                     /locus_tag="P5164_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44316.1"
                     /translation="MAIRRTLSLAGLALAGAFLLALEAPAYLIILKDGTRLEASAKPV
                     ASGRNYLFHDKLGAQKMIAISEVDPAKTEAANKENYRDAYILGDPEPMKKESDTSAKA
                     PSLSEYIRQNRKSQIEAPPVSAPVVPGGAPAAPRSAGRSEAAAAPAAVLDPIVQDSFL
                     RAYQTAAVRGTRLTPLAGGALRVQAVTDTETIVFGALVGTARGLKESRTAGRLVEKVE
                     LYMTTSAGENAGKFVILPEDAEALLNGTVPPAKFFVARVEL"
     gene            4069..5082
                     /gene="aroF"
                     /locus_tag="P5164_10250"
     CDS             4069..5082
                     /gene="aroF"
                     /locus_tag="P5164_10250"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRY44317.1"
                     /translation="MLIVMDRSATAADVGRVVEAAERMGLKAHPIPGEQRTAVGITGN
                     RGTVEPTAFENLPGVLEVIKVSHPYKLVSREFHPEDTIVSIGGVPVGGTRLAIIAGPC
                     AVETLEQTVTIAKEVKARGAHLLRGGAYKPRTSPYSFQGLGEEGLKILAAARDETGLP
                     VVTEALDTSSIDVVAKYADCLQIGARNMQNFELLKAAGRIGKPVLLKRGMSATLEEFL
                     LAAEYVLAEGNPNVVLCERGVRTFSDYTRNTLDLSVVPAVERLSHLPIVVDPSHGTGR
                     RDKVKPMSLASIAAGADGVAVEVHHQPETALSDGPQALTPPMFSDLMERLAPIAAAVG
                     RTL"
     gene            5079..6173
                     /gene="carA"
                     /locus_tag="P5164_10255"
     CDS             5079..6173
                     /gene="carA"
                     /locus_tag="P5164_10255"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRY44318.1"
                     /translation="MRGRPAVLLLEDGRAFEGEGFGAEGTAFGEVVFNTAMSGYEETL
                     TDPSYRGQIVVMTASHVGNYGVTGDDAESDRLQVAAFAARHVPERFSNARGKKSLGEA
                     LRESGTVGIEGLDTRAVVRHLRSLGAMRGAVSTEHEGAEARAALLAAVREQPAMTGAA
                     LALEVGTRSPYPFGAHAAGRPRVAAIDYGVKRNILRHLAARGLDVTVFPATAPASDLL
                     GFDGFFLSNGPGDPAALPACVETIRSLVGTGKPLFGICLGHQLLGLALGARTFKLKFG
                     HRGVNHPVKELATGAISITSQNHGFAVDPSSLPAGLELTHVNLNDGTCEGFRLAGRPV
                     FAVQYHPESAPGPHDSGALFDELARLLAAR"
     gene            6170..7399
                     /locus_tag="P5164_10260"
     CDS             6170..7399
                     /locus_tag="P5164_10260"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY44319.1"
                     /translation="MNVSLFLARRFLSGRRGSLLGTVSSLALGGVALGTAALVVAMGL
                     MSGYRSELFEKLAGTNAEVLVLPGVEADDAAVRTTLAGLPGVAAVAKTAFAPGLLLGP
                     KVPAGVDAVVKGLDVPTGLATTRLLGSDPAVAGRMRAPARGERVPVLLGSGLAARLGA
                     GAGSPLVLETASLSLARGVAPPRRTALVVAGIVTTGFTEVDEGWAVVPIGAFDRFAPP
                     DTRSGVWELKLVDPSRPDTVVEAARAALPAASVLDWRALNGDLFRALLVQQTLLFVAL
                     ALIVAVAAGTVVSSVVVLVAQKTREIGVLAAIGAGPRAVARTFRLAGLAVGGAGVLLG
                     AVAGVAICALLTATRAVRFPAEIARVYYVSWMPFRPEPRDVLAIAATGLLLVLLAAAF
                     PARRAARLAPAEALRYE"
     gene            7566..7802
                     /locus_tag="P5164_10265"
     CDS             7566..7802
                     /locus_tag="P5164_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44320.1"
                     /translation="MKKTTTAVVATIAALALGSSALATMQLQKEYKAKDPKANCASCH
                     VDKMPKKDKHDLNDLGKKVKGATGADGKVDWSKV"
     gene            7953..>8200
                     /locus_tag="P5164_10270"
     CDS             7953..>8200
                     /locus_tag="P5164_10270"
                     /inference="COORDINATES: protein motif:HMM:NF014552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HRY44321.1"
                     /translation="MSRLKIALAADHGGFDLKERLKEHLKAGGHDVADLGTNGKEPVD
                     YPVFARAAALLVSRGEADFGVVVDGAGIGSAMAANKVPG"
BASE COUNT         1116 a         2969 c         2976 g         1139 t
ORIGIN      
        1 tctcgcacct cggcctcgcc gtcccgtccg tcgacggcgg cggcgccatc tgggacctgc
       61 tcggcctcac cgagaagcac cgggaagagg tcgcctccca gaaggtgatc acctcgttcc
      121 gccccgtcgg ggagtcgagc ctcgagctcc tggagccgac cgcgcccgag agcccgatcg
      181 cgaagttcct cgagaagaag gggcccggca ttcaccacct ctgcctcgaa gtccccgacg
      241 tcgcggcgac gctcgagaag ctgaaggccg ccggcatccg cctcatcaac gagacgccgg
      301 tccccggagc ccacggctgc ctcgtggcgt tcatccaccc ggcctcgacc ggcggcgtcc
      361 tcctcgagct ctcccagccg gcccccgggc actgaccccg gccggagcgc gtcccggacg
      421 cgcgcgcgga gcgctcggcc gggacggcgc cctcagggga ccgtcgggat ctcggagccg
      481 cggacgaaac ggtcgaagaa cggagtccag tccttccgcg tgaccgcctc gaggacctcc
      541 tcgacccgct tcgtcgtccc gagccttccg ccgagcgtgg actggacgga ggaaaggaag
      601 gtcaggaacg cctcgtcgcc gatctcgcgg tggatcgccg cgaggacgag gggaccccga
      661 tcgtagagga gccaggtccg gaggttgtcg gcctggacct ggtcgttctt ggcccggagc
      721 cggtgggcga ggaggagcgg gaccgtctcc ttcgtctgcg ccgcgcggga gcgccagcgg
      781 gtcaggtaga ggtcgtaagc ctccttcccg gccgcctgcc ggatgaagaa ggcggagcag
      841 tactcggcga acgactcgga gagccactgc tcggagaggt cggggagcct caccccgttc
      901 ccccaccact ggtgggagag ctcgtgcgcg aacctctcgt tcacgcctgc ggcggcgcgg
      961 cggtaaagcc ctttccccat cgggttgaag gcttcggtgc cgatcaggag gagccccgcg
     1021 ggggcctgcc cctgggccca gacggccatc tcgacgatcg tcagctcgcg gaccggcatc
     1081 ttcccgagga agatctcgaa gaacgaggcc gtcgcgaagc cgaggtcgag gaggctgcgc
     1141 gacgcggtcc ggttccggcc gccgtaggag gcgacgcgga gggtccggcc gtccttcacc
     1201 tcctcctcca gggagtaggc gccggcgagg acggcgaggt tctgcgccgg cccgtcgagg
     1261 cgcgtctcga ggacgttccg ctccccctcc tccgcgcggc ggagcgtgac gcccggagcg
     1321 atggggtgga acggcttcgg gacgctcatc gtgacctgcg ccgagaaggc ccgcccggcc
     1381 atcggcgggg cggggaacca gggagcgagg ccgaggaccc agtagttgtt cccgaggggc
     1441 cggacgagga ggcccccttc gacctcgacg cggacgcggg cgctcttccc ctcggcgatc
     1501 ggcgcccgga gcgcgacggc gagctggtcg ttccggtggt cgaactcgag ggcgtttccc
     1561 gcctcgtcga agacgccggt caccgtcacg ggacgctcgc ggatccggtc gctgccgcgc
     1621 cggacgttgc cgaagagcgc cgtctgctgg atggtcgcgc cggtctgcga ttcggccgtg
     1681 tcgagcaggt cgaggaggag cgtgccgacg gcgcccctgc gggcctcgaa cgtctccgtc
     1741 gcggagatcg agacgtcgga gccgcccgag gcggtcacct tcaggtcgac gtgcgtcaga
     1801 acgacccggg gggagggcgg gtcgaacaga tcgccctcga tcggctgctc ggcgaggagg
     1861 atcgcgtagc tccggtcgtc gccgcggctc acccgtacct ggaaccgtcc gctcgcggtg
     1921 atccgctcgc ggcccgactc gagcggatcg tggaggaaga cgaggtcctc ccggccgccg
     1981 gtcgtctcgg cgcggacgac gcgcgcgccg ggcgcgtccc gccgctgcgc ggcccggagg
     2041 tgggcggacg gcggctcttt caccttcgcg gtgcggtccc ggtgctttcg aaaggcgggg
     2101 ccgagggacg gctccgtggc gggggagggg agctcgggga gcgtctgggc cggcgcgagc
     2161 cagaggagcg tctcgaacga gtccgcgatg gcgagaccgg cctcggtggc ggtggggctc
     2221 agccgggtgt tcttcgaaag cgtccagcgg acggcggaat gctcgcgggg gtcgcgcggg
     2281 acgtaggtga aggtgccctt tcccgagaga aacagccccg ccggtacccc gtcgggatcg
     2341 gtgacgacgg cggcgcgacc ctcggggagc cggatggtgg ccgcgccggc ggagaggacc
     2401 aggtccttca cgggccggct cgaatccggg acggagaggt cgtcgaccgc ccggaagagg
     2461 ctggaggtcg cgggctccgc ctccgccccg tggagggagc acggaagcgc gaggagggcg
     2521 agggcgaggg cgcgcgccgg gaaggaggtc atgccggatt ctgccctgcc gggctcccgg
     2581 gggcctattt cgggagcgtc acggagaccg tctcgccccc ttcgacgcgc gccgcgaggg
     2641 cgacggcgtc gtcggcggcc actccgtgct tgatcaggaa cgcgcggagg ggctcggccc
     2701 cgtcgaagaa gttcgtccgc ttctcggccc tgttgaaggc cgcgaaggcg tagggcgccc
     2761 cgtcggggcc tttccggagc tgccaaacga gctcggccac gggtcctcct tgctcactgg
     2821 agcggcccgt cgggccgcga ggaatcgagg cggaggacgt cgcggacgtc ccgccccacc
     2881 atctcgagga agcgctcgtg gtaggcggcg accgtcccga cgcgggagtc ctcgaacatc
     2941 ttctcgacgc ggtgaagcgg aaaaaagagg gtcgcgacgc ccagctcggg ctcgcccccc
     3001 gacaggaact gccgcgccca gtcgtcgaag agcgcgacgt cgatcccccg gaccgtcacg
     3061 cccgccgggg tgagcgcgcg gagcgccccc cagtacttct ccttcggcga gtggaggtgg
     3121 acgagcacga gcgtcccggc gtccacgtcg gcctcaggcg cccgccgccg gcagcgggct
     3181 cgtcgcgcgg aacgcgtcgg gcacggcggc ctcagagctc gacgcgggcg acgaagaact
     3241 tcgcgggcgg cacggtcccg ttcaggagcg cctcggcgtc ctcggggagg atgacgaact
     3301 tcccggcgtt ctctcccgcg ctcgtcgtca tgtagagctc gaccttctcg acgagccggc
     3361 cggccgtccg cgactccttc aggccccgag ccgtcccgac cagggcgccg aagacgatcg
     3421 tctcggtgtc ggtgacggcc tggactcgga gcgccccacc cgcgagcggc gtcaggcgcg
     3481 tgccgcggac cgcggcggtc tggtaggcgc ggaggaacga gtcctggacg atcgggtcga
     3541 ggacggccgc gggcgcggcg gcggcttcgg accgtccggc gctccgcggc gcggccggcg
     3601 ccccgcccgg caccacgggc gcggacacgg ggggcgcctc gatctgcgac ttccggttct
     3661 gccggatgta ctcggagagg gacggcgcct tcgcgctcgt gtccgattcc ttcttcatcg
     3721 gctcgggatc gcccaggatg taggcgtcgc ggtagttctc cttgttggcc gcctcggtct
     3781 tcgcggggtc cacctcggag atcgcgatca tcttctgggc cccgagcttg tcgtggaaga
     3841 ggtagttccg ccccgacgcg accggcttgg cggacgcctc gaggcgggtc ccgtccttga
     3901 ggatgatgag gtaggccggc gcttcgagcg ccaggaggaa cgcgcccgca agagcgagcc
     3961 ccgcgaggga gagcgtgcgc cggatcgcca tcgttgcctc ctcggcggcc gggaaggccc
     4021 ggcgcgccac gatgaagtat aaacagcggt tccggagggc gagacgccgt gctgatcgtc
     4081 atggacagga gcgcgaccgc ggccgacgtg ggccgggtgg tcgaggccgc cgagcggatg
     4141 gggctgaagg cccatcccat cccgggggag cagaggaccg cggtcgggat cacggggaac
     4201 cggggcacgg tcgagccgac ggccttcgag aacctccccg gggtcctcga ggtcatcaag
     4261 gtctcgcacc cctacaagct cgtctcgcgg gagttccacc ccgaagacac gatcgtctcg
     4321 atcggcggcg tccccgtggg cgggacgcgc ctcgcgatca tcgcgggccc ctgcgccgtc
     4381 gagacgctcg agcagacggt cacgatcgcg aaggaggtca aggcgcgcgg ggcgcacctc
     4441 ctccgcggag gggcttacaa gccccggacc tcgccctact ccttccaggg gctcggggag
     4501 gaggggctga agatcctcgc ggccgcgcgg gacgagacgg gactcccggt cgtcaccgag
     4561 gcgctcgaca cctcctcgat cgacgtcgtc gcgaaatacg ccgactgcct ccagatcggc
     4621 gcccggaaca tgcagaactt cgagctcctg aaggccgcgg ggcggatcgg caagccggtc
     4681 ctcctcaagc gcgggatgtc ggcgaccctc gaggagttcc tcctcgccgc cgagtacgtc
     4741 ctcgccgagg ggaacccgaa cgtcgtcctc tgcgagcggg gcgtccgcac gttcagcgac
     4801 tacacgcgga acacgctcga cctctcggtc gtcccggccg tcgagcgcct ctcccacctg
     4861 ccgatcgtcg tcgacccctc ccacggcacg ggacgtcgcg acaaggtcaa gccgatgtcg
     4921 ctcgcctcga tcgccgcggg cgccgacggc gtcgcggtcg aggtccacca ccagcccgag
     4981 acggcgctct cggacgggcc ccaggcactg acgccgccga tgttctccga cctgatggag
     5041 cggctcgcgc cgatcgccgc cgcggtggga cggaccctgt gagaggccgc cccgccgtcc
     5101 tcctcctcga ggacgggcgc gccttcgagg gcgagggctt cggggcggag gggacggcct
     5161 tcggcgaggt cgtcttcaac acggcgatgt ccggctacga ggagacgctg accgacccct
     5221 cctaccgcgg gcagatcgtc gtcatgacgg cctcgcacgt cgggaactac ggcgtgacgg
     5281 gcgacgacgc cgagtccgac cggctccagg tcgccgcctt cgcggcgcgg cacgtcccgg
     5341 agcgcttctc gaacgcgcgc gggaagaaga gcctcggcga ggcgctccgc gagtccggca
     5401 cggtcggcat cgagggcctc gacacgcgcg ccgtcgtccg gcacctgcgc tccctcggag
     5461 cgatgcgggg cgccgtctcc acggagcacg agggggctga ggcgcgcgcg gcgctcctcg
     5521 ccgccgtccg cgagcagccg gcgatgaccg gcgccgcgct cgcgctcgag gtcgggacgc
     5581 gatcgcccta cccgttcgga gcccacgccg cgggacggcc gcgcgtcgcc gcgatcgact
     5641 acggcgtcaa gaggaacatc ctccgccacc tcgccgcgcg cggcctcgac gtcacggtct
     5701 tcccggcgac ggcgccggcc tccgaccttc tcggcttcga cggtttcttc ctctcgaacg
     5761 ggccaggcga ccccgcggcc ctccccgcgt gcgtcgagac gatccgctcg ctcgtcggga
     5821 ccggcaagcc gctcttcggc atctgcctcg gccaccagct cctcggcctc gcgctcggcg
     5881 cgcggacctt caagctgaag ttcggccacc gcggcgtgaa ccatccggtg aaggagctcg
     5941 ccaccggggc gatctcgatc acctcgcaga accacggctt cgcggtcgac ccgtcctcgc
     6001 tcccagcggg gctcgagctg actcacgtga acctgaacga cggcacgtgc gagggattcc
     6061 gcctcgcggg ccgccccgtc ttcgccgtcc agtaccaccc cgagtcggcc ccggggccgc
     6121 acgattccgg cgcgctcttc gacgagctcg cgcggctcct cgccgctcgt tgaacgtctc
     6181 cctcttcctc gcccggcggt tcctttcggg gcggcgcggc agcctcctcg ggacggtctc
     6241 gtcgctcgcc ctcggcggcg tcgcgctcgg gacggcggcg ctcgtcgtcg cgatggggct
     6301 gatgtcgggc taccggagcg agctcttcga gaagctcgcc ggcacgaacg ccgaggtcct
     6361 cgtcctgccg ggcgtcgagg cggacgacgc ggcggtgcgg acgacgctcg cgggcctccc
     6421 gggagtcgcg gcggtcgcga aaaccgcctt cgcgcccggg ctcctcctcg ggccgaaggt
     6481 gccggccgga gtcgacgcgg ttgtcaaggg cctcgacgtc ccgaccggtc tcgccacgac
     6541 gcgcctcctc gggagcgacc cggcggtcgc cggacggatg agggcgcccg cccgtggcga
     6601 gagggtcccc gtcctcctcg gctcgggcct cgccgcgcgg ctcggcgccg gggcgggaag
     6661 ccccctcgtc ctcgagacgg cctccctctc cctcgcccgg ggcgtggcgc cgccgcgacg
     6721 gacggcgctc gtcgtggcgg ggatcgtcac gaccggattc acggaggtgg acgagggctg
     6781 ggccgtcgtc ccgatcggag cgttcgaccg cttcgccccg cccgacacgc ggagcggcgt
     6841 ctgggagctg aagctggtcg acccgtcccg accggacacg gtcgtcgagg cggcccgtgc
     6901 ggcgcttccc gcggcctccg tcctcgactg gcgcgccctg aacggagacc tcttccgcgc
     6961 cctcctcgtc cagcagacgc tcctcttcgt cgcgctcgcg ctcatcgtcg ccgtcgcggc
     7021 cggcacggtc gtctcctcgg tcgtcgtcct cgtggcgcag aagacccggg agatcggggt
     7081 cctcgccgcg atcggagccg gaccgcgcgc cgtcgcccgg acgttccggc tcgcggggct
     7141 ggcggtcggc ggggcgggcg tcctcctcgg cgcggtcgcg ggcgtcgcga tctgcgccct
     7201 cctcaccgcc acgcgagccg tccgcttccc ggcggagatc gcccgcgtct actacgtctc
     7261 ctggatgcct ttccggcccg agccgcgcga cgttctcgcg atcgcggcga ccgggctcct
     7321 cctcgtcctg ctcgccgcgg ccttcccggc ccgccgtgcg gctcggctgg ccccggcgga
     7381 ggcgctccgc tacgagtgag ccgttgcgga gatttgatct cagcgatcct ccccgggaga
     7441 tcgtccgtcc gcgatgacgg attggcctaa cgcaggtgga acgcctcttg cgaatcaggc
     7501 tccagcgagg ccgatttacg ttagcatttc ccgctcgtcg agactcgcag caggaggaac
     7561 aaggaatgaa gaagaccacc accgccgtcg tcgccacgat cgccgctctc gccctcggca
     7621 gctccgccct cgccacgatg cagctccaga aggagtacaa ggcgaaggac ccgaaggcga
     7681 actgcgcctc gtgccacgtc gacaagatgc cgaagaagga caagcacgac ctgaacgacc
     7741 tcggcaagaa ggtcaagggc gcgacgggcg ccgacggcaa ggtcgactgg tccaaggtct
     7801 gaggaccctc gaaagccggg cgcgtgaggc gccgtatcga acgctcccga gccccgcgcc
     7861 cgggggcgtt tcccttttcg ggcagccgag ggcacgagat ggtcgatcct cgcggcctct
     7921 ctcgacgtcg ctccgcgtag aatccccctc ccatgtcgcg gctgaagatc gcactcgccg
     7981 ccgaccacgg cggcttcgac ctcaaggagc ggctgaagga gcacctgaag gccggcggcc
     8041 acgacgtcgc cgacctcggg acgaacggga aggagccggt cgactacccg gtcttcgccc
     8101 gcgccgccgc gctcctcgtc tcccgggggg aggccgactt cggcgtcgtc gtcgacggcg
     8161 ccgggatcgg ctcggcgatg gcggcgaaca aggtccccgg
//
LOCUS       DAOTEZ010000171         8166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_6209_length_8166_cov_7.198866, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000171 DAOTEZ010000000
VERSION     DAOTEZ010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8166
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6209_length_8166_cov_7.198866"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..799
                     /locus_tag="P5164_10275"
     CDS             8..799
                     /locus_tag="P5164_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HRY44322.1"
                     /translation="MKLGIGGFLLLAVLSLVFKKDLFSLVGAGGMGPAADVSAGTGSA
                     GGAIDDPREEELVEFVSFVLDDAQATWTKILPAVGGEYSRAKLVLFRDGVDSACGYAG
                     SSTGPFYCPGDQKVYVDLGFYQELKDRFGAPGDFAQAYVIAHELGHHVQHLIGIDQAV
                     RQRQAADPRNENAYSVAMELQADCFAGVWGHETAQRGILERGDVEEGLNAAAAIGDDR
                     MQRAAGRRVTPDAFTHGSSEQRVAWFRKGLESGDIKACDTFGAMR"
     gene            complement(881..1138)
                     /locus_tag="P5164_10280"
     CDS             complement(881..1138)
                     /locus_tag="P5164_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016902710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44323.1"
                     /translation="MIHKLLGGLNLRFPGLFALLAAVFFVDLVVPDFIPFVDEIILAL
                     LTMLFALWKERRPTIVTRTNGVPPLPPPAQPPLPPAAGEER"
     gene            1301..1376
                     /locus_tag="P5164_10285"
     tRNA            1301..1376
                     /locus_tag="P5164_10285"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1334..1336,aa:Ala,seq:ggc)
     gene            complement(1446..2516)
                     /gene="rfbB"
                     /locus_tag="P5164_10290"
     CDS             complement(1446..2516)
                     /gene="rfbB"
                     /locus_tag="P5164_10290"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HRY44324.1"
                     /translation="MILVTGGAGFIGVNFVLGWLAEGDEPVVNLDKLTYAGNPRSLAA
                     IDGDPRYVFVKGDIGERALVEALLSRHRPRAVVNFAAESHVDRSIHGPEEFILTNVVG
                     TFHLLEAVRGYWSALPDAEKSAFRFLHVSTDEVYGSLGPADPPFTEENPYAPNSPYSA
                     SKASSDHLVRAYHHTYGLPTLTTNCSNNYGPYQFPEKLIPLMILNALRGKPLPVYGDG
                     MNVRDWLYVGDHCAAIRAVLANGRPGETYNVGGWNEMPNLVIVRTLCRLLDEARPDPA
                     GPRERLVTYVKDRPGHDRRYAIDARKIHRELGWKPAETFESGIRKTVSWYLENLPWVE
                     EVASGDYRRWIEANYGDRGAGA"
     gene            complement(2527..3561)
                     /gene="galE"
                     /locus_tag="P5164_10295"
     CDS             complement(2527..3561)
                     /gene="galE"
                     /locus_tag="P5164_10295"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRY44325.1"
                     /translation="MSSILVTGGAGFIGSHTTLELLRAGRDVVVLDSLVNSRREAVER
                     VAALAGRVVPFVEADVRDRAALDALFARYPVRAVIHFAGLKAVGESVEKPLAYWDANV
                     GGSVALLEAMTKAGVKTLVFSSSATVYGDPASVPIPEDAPLGMPSSPYGRTKLVVEQL
                     LSDVAASDPEWRIARLRYFNPVGADASGRIGEDPRGLPNNLMPYVAQVAVGRREALSV
                     YGDDYPTPDGTGVRDYIHVTDLALGHLAALAKLEGAPGLRTWNLGTGRGFSVLEVVRA
                     FEEASGRKVPYRVVPRRPGDIGRCWADPTRAERELGWRATRGLAEMCADAWRWQSTNP
                     DGYGGSDEKG"
     gene            complement(3647..5194)
                     /locus_tag="P5164_10300"
     CDS             complement(3647..5194)
                     /locus_tag="P5164_10300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY44326.1"
                     /translation="MPDRELRPAAPPAWGLLLAIAGARFALHAALSSRYGYFRDELYF
                     LDCGRHLAWGYVDAAPAIGLYARAALLLGGSLPVLRLLAGLAGAGVVLLTGLLAWRLG
                     GSRAAQGLASVAALATPIFLAIASLFSMNVVEPLFWTGCAIVVVRLADGASPRLWLLF
                     GLLAGLGLLNKHSTAFFGVAVFAGLLLTPMRRHLATPWPWAGAGVALLVFLPNVLWQA
                     ANGFPTLEDLRNVARTGKNVVLGPGEFVLTQVLQQGPHLLPLWLGGVAWLLFARSGRY
                     RALGAAFAVFFVLMFAMNAKGYYLTPVFPIVIAAGAVGLDEALERSRRLAGRAAPRRA
                     AAAWAVLLTLPAAPMVLPLLPPERLVAYMERLGFAPPKAEVAHEGPLPQMFGDQLGWP
                     ELVAEVAKVWNGLTPEERARGAIFANNYGEAGAINQFGPAHGLPRAISAHQNHFFWGP
                     RDFRGDVLVVLQDDRESLERLCASVEEAGRHHHPWGMAEENGPIWVCRGLKTPLPELW
                     PRLKHWN"
     gene            complement(5278..7845)
                     /locus_tag="P5164_10305"
     CDS             complement(5278..7845)
                     /locus_tag="P5164_10305"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRY44327.1"
                     /translation="MTSSARSRVPLRGLLLAGALLVSGALEGSEPCEAFRFAPPRDVM
                     PLAAAATVRAADVNGDGLVDLIAVEGSRVHGAVQRPDGTFSPLAPRDLGAHGLLVADV
                     DGDGTTDPVVLADDGPRRLWVSAEGRLEAGPPAVPPVAIPTRPIAVADLDGDGREEVL
                     VNARQTLDAWRMGSDGVFRREVVAPLALGAGSGLSLVAGDFDGDGDDDVVATAENYPT
                     FAPRGPLLWNDGRGVLAAGAPVELPEVWSTAVADFDGDGREEFVWAGVGTTAMSHYWM
                     ASLAWWDGANGLDGKHLDILSSPASLLSADFDRDGRPDLVVSGGYGRLHRGMGRDFGP
                     AVAVSGLSPTAAVDVDGDGYPDLVGRSENGVAIARNVCGTSLPDVSVPVVVSTSGANG
                     VRYETELTLDNASSTAVDLDLTWVPSFGGGAGSTTLRLEAAAQLFFPSVLDALADAGI
                     PVAREGDRGGSLAIRVRRGNPASVTATARVVAVGAGRGGVGFAERPLGAGLAPTSIVG
                     WLREAGGDRSNLAVVNLGGEREGDVVLRVTLVSGESPSRSVVLPEVRLAPGTFHQWNR
                     VLAGAGFTSGWALVTRVSGKAPFFAWGVVNDEGTGDGSFVAGFESDLPRAASLVVPAI
                     VESGRYATDLILTNTRNEAVILSGRLVSETLSAYDRTARFRLEVPPFGQLFLSDFVER
                     LRGEGVAGLPPRGGEVVGALFLDVEEGSPEGLFVGARVRAPKPEGRYGVFYEAAPADR
                     PARQEGWSAALRHDGSVRTNVAIVNLEDETIVCRLTVRDPARGGAAAGDSRTLTIGPH
                     RWVQVASVLEALGTGLERGRARVEPVGRPARFLAYGVLNEGARPGEGSDDGTYLPGR"
     gene            complement(7904..>8166)
                     /locus_tag="P5164_10310"
     CDS             complement(7904..>8166)
                     /locus_tag="P5164_10310"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HRY44328.1"
                     /translation="PFRFRVRFAKAMAAWVAEVRWHPKQKLTPLPSGEIELELPAGSL
                     FEARRFVLSFGRDAVALSPPELVAALRGETEEMAAAYRGAKG"
BASE COUNT         1164 a         2849 c         3113 g         1040 t
ORIGIN      
        1 gccggggatg aagctcggca tcggcggctt cctgctgctc gccgtgttga gcctcgtttt
       61 caagaaggac ctcttctccc tcgtcggcgc gggcggcatg ggtcccgccg ccgacgtctc
      121 ggcgggaacg ggctccgcgg ggggcgcgat cgacgacccg cgcgaggagg agctcgtcga
      181 gttcgtctcc ttcgtcctcg atgacgcgca ggcgacctgg acgaagatcc tgccggcggt
      241 gggcggggag tattcgcggg ccaagctcgt cctcttccgc gacggcgtcg actcggcctg
      301 cggctacgcg ggctcgtcca ccgggccgtt ctactgcccc ggcgaccaga aggtctacgt
      361 cgacctcggc ttctaccagg agctgaagga ccggttcgga gcgcccggcg acttcgcgca
      421 ggcctacgtc atcgcgcacg agctcggcca ccacgtgcag cacctgatcg gcatcgacca
      481 ggccgtgcgg cagcgtcagg cggcagaccc gcggaacgag aacgcctact ccgtcgccat
      541 ggagctccag gccgactgct tcgccggggt ctgggggcac gagaccgcgc agcgcgggat
      601 cctcgagcgg ggcgacgtgg aggaggggct gaacgcggcc gcggcgatcg gcgacgaccg
      661 gatgcagcgg gccgcggggc ggcgggtcac gcccgacgcg ttcacgcacg gctcctccga
      721 gcagcgggtc gcctggttcc ggaagggcct cgagtcgggc gacatcaagg cctgcgacac
      781 gttcggcgcg atgcggtgag ccggcgagcc cgcgggcgcg gaaggcccgc gcggctccgg
      841 aagcgggctc ggaggcgccg cggcggcgcc tccgagctcc tcagcgctct tcgcccgccg
      901 cgggcggcag cggaggctgc gcgggaggcg ggagcggcgg gacgccgttc gtcctcgtga
      961 cgatcgtcgg tctccgctcc ttccagagcg cgaagagcat cgtcaggagc gcgaggatga
     1021 tctcgtcgac gaacgggatg aagtcgggga cgacgaggtc gacgaagaag acggccgcca
     1081 ggagggcgaa gagccccggg aagcggaggt tcaggccgcc cagcagcttg tggatgatcc
     1141 ccctcctccg gagggcctcg gggtcgcgcg ccggggattc catggcggaa tcatagggaa
     1201 gtacggccgg gatgtccggc cgcttgacca gggcgagcgg aggatggtag tttccccggt
     1261 ccgcggaggc gttcgcgcct ccccgttctt tcgaaagcgt ggggctgtag ctcagttggg
     1321 agagcgcctg aatggcattc aggaggtcga cagttcgatc ctgttcagct ccaccatttc
     1381 attccacgtg agggtactcg ccgggcatgt cccggcgttt ctgtttctgc ggggcgccgg
     1441 gtccgctacg cgcccgctcc ccggtccccg tagttcgcct cgatccagcg gcggtagtcg
     1501 ccgctcgcga cctcctccac ccaggggagg ttctcgaggt accaggagac ggtcttccga
     1561 atgccgctct cgaacgtctc ggccggcttc cagccgagct cgcggtggat cttccgcgcg
     1621 tcgatcgcgt agcgacggtc gtgcccgggg cggtccttga cgtaggtgac gagccgctcg
     1681 cgcgggccgg ccgggtcggg ccgggcctcg tcgaggagcc ggcagagcgt cctcacgatg
     1741 acgaggttcg gcatctcgtt ccagccgccg acgttgtagg tctctcccgg ccggccgttc
     1801 gcgaggacgg cgcggatcgc ggcgcagtgg tcgccgacgt agagccagtc gcggacgttc
     1861 atcccgtcgc cgtagaccgg gagcggcttg ccccggagcg cgttcaggat catgagcggg
     1921 atcagcttct cggggaactg gtacgggccg taattgttcg agcagttcgt cgtgagcgtc
     1981 ggcaggccgt aggtgtggtg gtaggcccgc acgaggtggt ccgaagaggc cttgctcgcc
     2041 gagtaggggc tgttcggggc gtacgggttc tcctcggtga agggcgggtc cgccgggccg
     2101 agcgagccgt agacctcgtc ggtcgagacg tggaggaagc ggaacgcgga cttctcggcg
     2161 tcgggaagcg cgctccagta gccgcggacc gcttcgagga ggtggaacgt gccgacgacg
     2221 ttcgtgagga tgaactcctc gggcccgtgg atcgagcggt cgacgtgcga ctcggccgcg
     2281 aagttgacga cggcgcgggg gcggtgacgg gagaggagcg cctcgacgag ggccctttcg
     2341 ccgatgtccc ccttcacgaa gacgtagcgg gggtcgccgt cgatcgccgc gaggctccgg
     2401 gggttccccg catacgtcag cttgtcgagg ttcacgaccg gttcgtcgcc ttcggcgagc
     2461 cacccgagga cgaagttcac gccgatgaag ccggcgccgc ccgtcacgag gatcattcgc
     2521 tcctcctcag cccttctcgt ccgacccgcc gtagccgtcg gggttcgtcg actgccagcg
     2581 ccaggcgtcg gcgcacatct ctgcgaggcc ccgcgtcgct ctccaaccga gctcccgttc
     2641 tgccctcgtc gggtcggccc agcagcggcc gatgtcgccg ggccggcgcg ggacgacccg
     2701 gtacgggacc ttccgacccg aggcctcctc gaacgcccgg acgacctcga ggacgctgaa
     2761 cccgcggccc gtgccgaggt tccaggtccg gagacccggc gctccttcga gcttcgcgag
     2821 ggcggcgagg tggccgagcg cgagatcggt cacgtggatg tagtcgcgca cgccggtgcc
     2881 gtccggcgtc gggtagtcgt ctccgtagac cgacagcgcc tcgcggcggc cgaccgcgac
     2941 ctgtgcgacg tagggcatga ggttgttcgg caggccgcgc gggtcctcgc cgatccgccc
     3001 gctcgcgtcg gcgccgaccg ggttgaagta ccgcagccgg gcgatcctcc actccgggtc
     3061 ggacgcggcg acgtccgaga ggagctgctc gacgacgagc ttcgtccggc cgtacgggct
     3121 cgacggcatg ccgagaggcg cgtcttccgg gatcggaacc gaagccgggt cgccgtagac
     3181 cgtcgccgag gagctgaaga cgagcgtctt cacgccggcc ttcgtcatcg cctcgaggag
     3241 cgcgacgctc ccgccgacgt tcgcgtccca gtaggcgagc ggcttctcga ccgactcccc
     3301 gacggccttc aggccggcga agtggatcac ggcccggacc ggataccgcg cgaagagcgc
     3361 gtcgagagcg gcacggtcgc ggacgtcggc ctcgacgaac gggacgacgc gtcccgccag
     3421 cgccgcgaca cgctcgacgg cctcgcgccg ggagttgacg aggctgtcga ggacgacgac
     3481 gtcccggccc gcacgcagga gctcgagcgt cgtgtgggag ccgatgaatc cggcgccgcc
     3541 ggtgacgagg atgctgctca tgcgtcgtcc gaagccggga agctgccttt ccgcgcgccc
     3601 ggcgcggcgg aacattagca ggtctcccgg gccgcgcggc cgacgctcag ttccagtgct
     3661 tcagccgggg ccagagctcg gggagcggag tcttcaggcc tcggcagacc cagatcgggc
     3721 cgttctcctc ggccatgccc caggggtggt ggtgccggcc ggcctcttcc accgaagcgc
     3781 agagtcgctc gaggctctcg cggtcgtcct ggaggacgac gaggacgtcg ccgcggaagt
     3841 cgcgcggccc ccagaagaag tggttctggt gggccgagat cgcgcgcggc aggccgtgcg
     3901 ccggcccgaa ctggttgatc gcccccgcct cgccgtagtt gttcgcgaag atcgcgccgc
     3961 gggcgcgctc ctccggcgtc aggccgttcc agaccttcgc cacctcggcc acgagctcgg
     4021 gccagccgag ctggtctccg aacatctgcg ggagcggccc ctcgtgcgcg acctcggcct
     4081 tcggcggcgc gaagccgagg cgctccatgt acgcgacgag ccgctcgggc ggcaggagcg
     4141 ggaggaccat cggagccgcg gggagcgtga ggaggacggc ccacgccgcg gccgcgcggc
     4201 gcggcgccgc gcggcccgcg aggcgccgcg atcgctcgag cgcctcgtcg aggccgacgg
     4261 cccccgccgc gatcacgatc gggaagaccg gcgtcaggta gtaccccttc gcgttcatcg
     4321 cgaacatcag gacgaagaag acggcgaacg cggcgccgag cgcccggtag cggccgctcc
     4381 gcgcgaagag gagccacgcg acgccgccga gccagagcgg gaggaggtgc ggtccctgct
     4441 ggagcacttg cgtgaggacg aactcgcccg ggccgaggac gacgttcttc ccggtccggg
     4501 cgacgttgcg caggtcctcg agcgtcggga agccgttcgc ggcctgccag agtacgttcg
     4561 ggaggaagac gagaagcgcg acgccggcgc cggcccacgg ccagggcgtc gcgaggtgcc
     4621 tccgcatcgg cgtcaggagg agcccggcga agacggcgac gccgaagaag gccgtggagt
     4681 gcttgttgag gaggccgagc ccggcgagga ggccgaacag gagccagagc cgcggggagg
     4741 cgccgtcggc gaggcggacg acgacgatcg cgcagcccgt ccagaagagc ggctcgacga
     4801 cgttcatcga gaagagggaa gcgatggcga ggaagatcgg cgtcgcgagc gccgcgacgg
     4861 aggcgagccc ctgcgccgcg cggctcccgc cgaggcgcca cgcgaggagg ccggtgagga
     4921 ggacgacgcc cgcgccggcg agcccggcga gcagccggag gacggggagc gagccgccga
     4981 ggaggagcgc ggccctcgcg tagagcccga tcgccggcgc cgcgtcgacg tagccccagg
     5041 cgaggtgccg tccgcagtcg aggaagtaga gctcgtcgcg gaagtagccg taccggctcg
     5101 agagggccgc gtggagcgcg aacctcgccc cggcgatcgc gaggaggagt ccccacgcgg
     5161 ggggcgcggc cggacggagc tccctgtcgg gcatgcccga atggtacggc gcgctcctcc
     5221 cggggttgcg ggggtgcccg ccgagtggac gtccctcgac ggccgacgga agagtcgtca
     5281 ccgccccggc aggtaggtcc cgtcgtcgct cccctcgccg gggcgtgccc cctcgttcag
     5341 gacgccgtag gcgaggaacc gggccggccg tcccaccggc tccacgcggg cccgcccgcg
     5401 ctcgagcccg gtcccgaggg cctcgaggac ggaggcgacc tggacccagc ggtgcgggcc
     5461 gatcgtcagc gtccgcgaat ccccggcggc ggccccaccg cgggccgggt cgcggaccgt
     5521 gaggcggcag acgatcgtct cgtcctcgag gttcacgatc gcgacgttcg tccggacgga
     5581 gccgtcgtga cggagggcag cggaccagcc ctcctggcgg gcgggccggt cggcgggggc
     5641 cgcctcgtag aagaccccgt atcgcccctc cggcttcggg gcgcggacgc gcgcgccgac
     5701 gaagaggcct tcgggagaac cctcttcgac gtcgaggaag agcgccccga cgacctcacc
     5761 gccccggggc gggaggccgg caacgccctc gccccggagt cgctcgacga agtcggagag
     5821 gaagagctga ccgaacgggg ggacctcgag gcggaagcgc gccgtgcggt cgtaggccga
     5881 gagcgtctcg gagacgagcc gcccggaaag gatgacggcc tcgttccgcg tgttcgtcag
     5941 gatcaggtcg gtcgcgtagc gtcccgactc cacgatcgcc gggacgacga gcgaggccgc
     6001 tcgcgggaga tcggactcga agcccgcgac gaacgagccg tcgcccgtcc cctcgtcgtt
     6061 cacgacgccc caggcgaaga agggcgcctt cccggaaacg cgcgtgacga gggcccagcc
     6121 ggaggtgaag ccggcgccgg cgaggacgcg gttccactgg tggaacgtgc ccggcgcgag
     6181 gcggacctcc gggaggacga cgctccgcga gggcgactcg cccgagacga gcgtcacgcg
     6241 caggacgacg tcgccctccc gttcgccgcc gaggttgacg acggcaaggt tgctccggtc
     6301 gccgccggcc tcgcgcagcc agccgacgat cgacgtcggc gcgagccctg cgccgagcgg
     6361 gcgctcggcg aagccgacgc cgccgcgccc cgcgccgacg gcgacgacgc gcgccgtcgc
     6421 ggtgacgctc gcaggattcc cgcggcggac ccggatcgcg agcgagccgc cgcggtctcc
     6481 ctcgcgcgcc accgggatgc ccgcgtcggc gagggcgtcg aggacggacg ggaagaagag
     6541 ctgcgcggcg gcctcgaggc gcagcgtcgt cgatccggcg ccgccgccga atgacgggac
     6601 ccaggtgagg tcgaggtcga cggccgtcga agaggcgttg tcgagcgtca gctccgtctc
     6661 gtaacggact ccattcgccc cggacgtcga gacgacgacc ggcacgctca cgtcggggag
     6721 cgacgtgccg cagacgttcc gggcgatcgc gacgccgttc tcgctccggc cgacgaggtc
     6781 ggggtagccg tcgccgtcga cgtcgaccgc cgcggtcggc gagagcccgg agaccgctac
     6841 ggccggcccg aagtcgcgcc ccatcccgcg gtggaggcgc ccgtacccgc ccgagacgac
     6901 gaggtcgggc cgaccgtcgc ggtcgaagtc ggcggagagg agcgacgccg gcgagctcag
     6961 gatgtcgagg tgctttccgt cgaggccgtt cgcgccgtcc caccacgcga ggctggccat
     7021 ccagtagtgg gacatcgccg tcgtgccgac gccggcccag acgaactcct cgcggccgtc
     7081 gccgtcgaag tcggcgacgg ccgtgctcca gacctcgggg agctcgaccg gggcgccggc
     7141 cgcgaggacg ccgcgcccgt cgttccagag gagcggcccc cgcggcgcga acgtcgggta
     7201 gttctcggcc gtcgcgacga cgtcgtcgtc gccgtccccg tcgaagtctc ccgcgacgag
     7261 ggagaggccg ctccctgctc cgagagcgag cggtgcgacc acctcccggc ggaagacccc
     7321 gtcgctcccc atccgccagg cgtcgagcgt ctgccgcgcg ttcacgagga cctcctcgcg
     7381 cccgtcgccg tcgaggtcgg cgacggcgat cggacgggtc gggatcgcga cgggcggcac
     7441 ggcgggcggc ccggcctcga gccgcccctc cgccgagacc cagagccggc ggggcccgtc
     7501 gtcggcgagg acgaccgggt cggtcgtgcc gtctccgtcg acgtcggcaa cgaggaggcc
     7561 gtgggcgccc aggtcgcggg gcgcgagggg cgagaaggtc ccgtcgggcc gctggactgc
     7621 gccgtgaacg cggctccctt cgacagcgat gaggtcgacg aggccgtctc cgttgacgtc
     7681 ggcggcgcgg acggtggcgg cggccgcgag cggcatcacg tcgcgcgggg gcgcgaagcg
     7741 gaacgcctcg cacggctcgg acccctcgag cgcgccggag acgaggagcg cgccggcgag
     7801 gagcagaccg cgcagcggga cccgggagcg agcggacgac gtcatcggag gcctcctcgt
     7861 cccggagtgt cgcctcgggc gggcagcttt cgcgcgcgcg ccctcaaccc ttcgcgcccc
     7921 ggtacgccgc ggccatctcc tccgtctcgc cgcggagcgc cgcgacgagc tcggggggcg
     7981 agagggcgac ggcgtccctc ccgaacgaga ggacgaatcg gcgcgcctcg aagagcgatc
     8041 cggccggaag ctcgagctcg atctccccgg aagggagcgg cgtcagcttc tgcttcgggt
     8101 gccagcggac ctcggcgacc cacgccgcca tcgccttcgc gaagcggacc cggaagcgga
     8161 acggct
//
LOCUS       DAOTEZ010000172         8154 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_6222_length_8154_cov_6.464131, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000172 DAOTEZ010000000
VERSION     DAOTEZ010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8154
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6222_length_8154_cov_6.464131"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="P5164_10315"
     CDS             <1..185
                     /locus_tag="P5164_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HRY44329.1"
                     /translation="GCPMGDAPENGVIDHRHRVFGYDGLWVVDGSAISANPGVNPSLT
                     ITALAERAMTFVPPKE"
     gene            239..1006
                     /locus_tag="P5164_10320"
     CDS             239..1006
                     /locus_tag="P5164_10320"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HRY44330.1"
                     /translation="MKPAAVLTDLDGTLLEHGGVLGAEARAAISRLRGLGVPVVPLTS
                     KTETELRALLAELDSGGIGSFENGAGIVTPSGAVVSEKAVPADRLSDRLDELARSAGV
                     PLTPAAALPVAEIRRITGLPEEKVPAMLARGFGLPFLAPEGAGPALEAAARGLSGARL
                     TRGGQFWHLSGEHGKEDAVELLVRSGAAARPFAGLGDAPNDAGFLALCDAAVLLPRAA
                     GGVDPALAAAVPGARVAPFPTGRGWAAALDRLLAEDE"
     gene            1003..2277
                     /locus_tag="P5164_10325"
     CDS             1003..2277
                     /locus_tag="P5164_10325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY44331.1"
                     /translation="MTAHALPPLAPEVAEAIRRIGRVDLLVGIPSFNNERTIGHVVRA
                     VVAGLARHFPGAKAVLVNSDGGSTDRTREIVERTEVGSLAEILVDAVPTGATTRIVTP
                     YHGIPGKGSAFRTIFRIAAELEAEACCVLDSDLRSITPEWVELLLEPVVSHHFDFVAP
                     LYARHRYDGTITNTIVYPLTRALYGKRVRQPIGGDFGFTGDLAAHFLEFGDWDGPVAQ
                     YGIDIFMTTTALAEGRKVAQSFLGAKIHDPKDPGSDLSPMMVQVVSTLFTLMERHEPA
                     WKRVAGSEKVPVFGFPYTVGLEPVPVKLDRLLAQYRQAAHDLPPIWESFLSPGSLAAI
                     REAAHAPAVSLGDEAWVRIIAEAAAGFRRRALPPATLIKSLVPLYLGKVATFVAETEA
                     GTAEEAEARLEELALAYERGKDGLEALWGKVP"
     gene            2289..2987
                     /locus_tag="P5164_10330"
     CDS             2289..2987
                     /locus_tag="P5164_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44332.1"
                     /translation="MGKNLGQQIALAFERLLDAFLSVVPAALVLLAAALVGVLTGFVL
                     RALLRWAIRLRRRSRRDEPSSSRQILRAAGLKADPERVAGTVSFWTAVVVALAVGVNA
                     LEPGALRNVLSEAVGFLPRLLGAMLLAIVGLGAGALVRRSVLLGAVNAGLPWARAGAR
                     AAYVLVVTSFVAAALDHLGVGRSILVAAFSIAGGAIALTLALAFGLGARDLARGWLEK
                     KLRAEEEDSGIRHV"
     gene            3069..5387
                     /locus_tag="P5164_10335"
     CDS             3069..5387
                     /locus_tag="P5164_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative monovalent cation/H+ antiporter subunit
                     A"
                     /protein_id="HRY44333.1"
                     /translation="MIVAVLSTFAVAALAPLLEKRLGRRAAPLLALVPAGLFVSLARH
                     VPEVSAGGARTDALGWIAELGVSLSFRLDGLSLLMALVVTGVGAVVLLYAGSYMEGHP
                     RRGNLLGYLLAFLAAMLGLVLSDNLVGLFVFWEATSLLSYVLIGFEREKAEVRASALQ
                     ALLVTGGGGLALLAGFVLLGQAGGSLEIGALAANAEAVKASPLYLPALLLVLLGAFTK
                     SAQVPFHFWLPDAMAAPAPVSTFLHSATMVKAGVFLLARLHPTLGGTPEWTGIVTTVG
                     AATMLTGALLAVGQRDLKRLLAYSTVSALGSLVMLVGVGTPAALAAAMVLLLAHALYK
                     GALFLVAGIVDHETGTRDVASLGGLFRALPVTAVAAAAAAFSMAGLPPLLGFVSKELL
                     YDAALRASPAPLLLVPVAVGSNMLLVAVALSVGWGPFTGRAGETPKAPHEAPAAMWAG
                     PLLLGLGSLAAGLFSGAISPFVSSAAAAALGAPHALTLELWHGFNLVLAKSLATLVVG
                     VALFAARGRIRSLAGSLAPLAAFGPGRLYRAGLAGLVRLAKGTAALLSSGSLRQDLRI
                     VLAATVALAAPLLLTRAVLGAPDALRSVRPLEAVLALLVVAGAVATLLAGSRLAAVVA
                     LGVTGFGVALLFLVFGAPDLAMTQFAVETLSVLLFVAVLRRLPRLQSRSAPRARVVDA
                     LLAGAVGAVMTGLVLAVHAEPLRSSLAPFFRESSLALAKGRNVVNVILVDFRGLDTLG
                     EITVLGVAALGVFSLVRLRIASRAAGARGDRS"
     gene            5384..5800
                     /locus_tag="P5164_10340"
     CDS             5384..5800
                     /locus_tag="P5164_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit B"
                     /protein_id="HRY44334.1"
                     /translation="MRSVILAAATRVVFPLLLLFSVFLLFRGHNEPGGGFVGGLVAAT
                     AYALLALSGGVASARRVLPARPEVLIGTGLLAALASGLVGLAAGRPFMTGLWGDVSLP
                     VVGKPGTPVLFDAGVYLTVLGVVLLILFTLQEEDEA"
     gene            5797..6141
                     /locus_tag="P5164_10345"
     CDS             5797..6141
                     /locus_tag="P5164_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HRY44335.1"
                     /translation="MTLLLALAIGALYAAGTYLLLRRSAIRVVFGLALLGYASNLLVF
                     TAGRLSREGPPIVPEGAATAAATSADPLPQALVLTAIVIGFGVQAFALVLLKRTCQEA
                     GTDDVDAMREET"
     gene            6138..7649
                     /locus_tag="P5164_10350"
     CDS             6138..7649
                     /locus_tag="P5164_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011456809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit D"
                     /protein_id="HRY44336.1"
                     /translation="MNLLLVVPLLVPLATAAATILLARSPRAVRAASFAGAAAFLAAA
                     VALLGATLDGTILVTQAGAWAAPFGISLVADRFAAAMVLVAAVLLAAVVAYSVRGLDG
                     ARERAGYHPLLHVLSMGVSGAFLTGDLFNLYVWFEVMLMGSFVLVSLGGERAQLQGAL
                     KYVVLNLVSSALFLAGAGLLYGSVGTLNMADAAVALRELPAQGLPSVVASLFLLAFGI
                     KAAAFPLFFWLPASYHTPPMPVAALFAGLLTKVGVYALVRVLTLVFVRDTAFTGPLLL
                     AVAALTMVTGVLGAMAQGDVRKILSFHIVSQIGYMLMGLGLGTRLALAGTVFYVLHHI
                     VVKANLFLVAGAMRRAGGSFSLDRLGSLLARSPLLAAAFLVPAVSLAGLPPLSGFWAK
                     YLLVRAGLEAGNGTIVAVALAVSLLTLFSMTKIWNEAFWKPAPAGADAAPAPLTTAER
                     ASLLGPVLVLGAVTVAIGLGAGVLYRFADAAAAQLLDPDLYVRAVLGARDGRM"
     gene            7653..8117
                     /locus_tag="P5164_10355"
     CDS             7653..8117
                     /locus_tag="P5164_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HRY44337.1"
                     /translation="MGTFAFLVFAWVAVTGEVTVGNLLEGAVLAGLLVLLLRIPVRRR
                     VRLEQVPKALGLLLYFLKEIVLSNAAVARSLLSPVSSLSPGIVAVPLDLKSDAGITVL
                     ANLVTLTPGTLSLDVSPDRTTLYVHALHVEDPEAFRREVKEGFERRVKEVFE"
BASE COUNT          816 a         3220 c         2796 g         1322 t
ORIGIN      
        1 gcggctgccc gatgggcgac gcgccggaga acggcgtcat cgaccatcgc caccgcgtct
       61 tcggctacga cgggctctgg gtcgtcgacg gctccgcgat ctccgcgaac cccggcgtga
      121 acccctcgct cacgatcacc gcgctcgccg agcgcgcgat gacgttcgtc ccgccgaaag
      181 agtagcgggc gaccggtttc ggtcgcgccg cgcggctcgc cggggtatac agggccggat
      241 gaagcccgcc gcagtcctca ccgacctcga cgggacgctc ctcgagcacg ggggagtcct
      301 cggagcggag gcgcgcgcag cgatctcgcg cctccgcggg ctcggggtgc cggtcgtccc
      361 gctcacgagc aagaccgaga ccgagctgcg cgcgctcctc gcggagctcg actcgggcgg
      421 gatcgggagc ttcgagaacg gagcgggaat cgtcacgccg tccggagccg tcgtctcgga
      481 gaaggcggtc ccggccgacc ggctctccga ccgcctcgac gagctggcac ggagcgcggg
      541 cgttccgctc acgcccgcgg cggcgcttcc cgtcgcggag atccggcgga tcacgggact
      601 tccggaggag aaggtccccg cgatgctcgc gcgcgggttc gggctcccgt tcctcgcgcc
      661 cgaaggggcg gggccggcgc tcgaagcggc ggcgcggggc ctctccggag ctcgcctcac
      721 gcggggcggg cagttctggc acctctcggg cgagcacggc aaggaagacg ccgtcgagct
      781 cctcgtcagg tccggcgccg cggcgcgccc cttcgccggg ctcggcgacg cgccgaacga
      841 cgccggcttc ctcgcgctct gcgacgccgc cgtcctcctg ccgcgcgcgg cgggcggcgt
      901 cgaccccgcg ctcgccgcgg ccgtgccggg cgcgcgcgtc gcccccttcc cgacgggccg
      961 cggctgggcc gccgccctcg accggctcct cgcggaggac gaatgacggc gcacgccctt
     1021 ccgcccctgg cgcccgaggt cgccgaggcg atccgccgga tcggacgcgt cgacctcctc
     1081 gtcggcatcc cctcgttcaa caacgagcgg acgatcggcc acgtcgtgcg ggccgtcgtc
     1141 gcggggctcg cgcgccactt ccccggtgcg aaggcggtcc tcgtcaactc cgacggcggc
     1201 tcgacggacc ggactcgcga gatcgtcgag cggacggaag tggggagcct cgcggagatc
     1261 ctcgtcgacg cggtcccgac gggagcgacg acgcggatcg tcaccccgta ccacggcatc
     1321 ccggggaagg ggagcgcgtt ccggacgatc ttccggatcg ccgccgagct cgaggcggag
     1381 gcctgctgtg tcctcgacag cgacctccgc tcgatcaccc cggagtgggt ggagctcctc
     1441 ctcgagccgg tcgtttccca ccacttcgac ttcgtcgcgc cgctctacgc gcggcaccgg
     1501 tacgacggga cgatcacgaa cacgatcgtc tacccgctca cgcgcgccct ctacggcaag
     1561 cgggtccgcc agccgatcgg cggggacttc ggcttcacgg gcgacctcgc cgcccacttc
     1621 ctcgagttcg gcgactggga cgggccggtc gcgcagtacg gcatcgacat cttcatgacg
     1681 acgacggcgc tcgccgaggg gcggaaggtc gcgcagtcgt tcctcggcgc gaagatccac
     1741 gaccccaagg accccgggtc ggacctctcg ccgatgatgg tccaggtcgt ctcgacgctc
     1801 ttcacgctca tggagcggca cgagccggcc tggaagcggg tcgcgggatc ggagaaggtg
     1861 ccggtcttcg gcttccccta caccgtcggc ctcgagccgg tcccggtcaa gctcgaccgg
     1921 ctcctcgcgc agtaccgcca ggcggcgcac gacctcccgc cgatctggga gtcgttcctc
     1981 tcgcccggct cgctcgccgc gatccgcgag gcggcccacg cgccggccgt ctccctcggc
     2041 gacgaggcgt gggtgaggat catcgccgag gcggcggcgg gcttccggag gcgcgccctc
     2101 ccccccgcga cgctgatcaa gtcgctcgtt ccgctttatc tcgggaaggt ggcgacgttc
     2161 gtcgccgaga cggaggcggg gacggccgag gaggccgagg cccgcctcga ggagctcgcc
     2221 ctcgcctacg agcgcggtaa ggacgggctc gaggccctct gggggaaggt cccctgacgg
     2281 agacgacgat gggaaagaac ctcggacagc agatcgccct cgccttcgag cgcctcctcg
     2341 acgcgttcct ctcggtcgtc ccggccgcgc tcgtcctcct ggcggccgcg ctcgtcggcg
     2401 tcctgaccgg cttcgtcctg cgcgccctgc tgaggtgggc catccgcctc cggcggagat
     2461 ctcgacggga cgagccctcc tcctcgcggc agatccttcg cgccgcgggc ttgaaggccg
     2521 acccggagcg tgtcgccggg accgtctcgt tctggacggc cgtcgtcgtc gccctggccg
     2581 tcggcgtgaa cgcgctcgag ccgggggcgc tccggaacgt cctctcggag gcggtcggct
     2641 tcctgccgcg tcttctcggg gcgatgctcc tcgcgatcgt cgggctcggc gccggcgcgc
     2701 tcgtccgccg gagcgtcctc ctcggcgcgg tgaacgccgg gctcccctgg gcgcgcgccg
     2761 gcgctcgtgc cgcctacgtc ctcgtcgtca cgtcgttcgt ggcggccgcg ctcgaccacc
     2821 tcggggtcgg gcggtcgatc ctggtcgcgg cgttctccat cgccgggggc gcgatcgccc
     2881 tcacgctcgc cctcgccttc ggcctcggcg cgcgcgacct ggcccgcggc tggctcgaga
     2941 agaagctgcg ggcggaggag gaggactccg ggatccgtca cgtctgaggc gatgcgtccg
     3001 gagcgcagcg ccgctcggtc tccgcggggc ggaatagcgc gggccgtccg cgcgttctac
     3061 aggatctcgt gatcgtcgcc gtcctctcca cgttcgccgt cgccgcgctg gcacccctcc
     3121 tcgagaagcg cctcgggcgc cgcgccgcgc cgctgctggc gctcgtcccg gcgggcctct
     3181 tcgtctccct cgcccggcac gtcccggagg tctcggccgg aggggctcgc accgacgcgc
     3241 tcggctggat cgcggagctg ggcgtctccc tctcgttccg gctggacggc ctctcgctcc
     3301 tcatggccct cgtcgtcacc ggcgtcggcg cggtcgtcct cctctacgcc gggagctaca
     3361 tggaggggca cccgcgccgc ggcaacctgc tcggctacct cctcgccttc ctcgcggcga
     3421 tgctcgggct cgtcctctcg gacaacctcg tcggcctctt cgtcttctgg gaggcgacga
     3481 gcctcctctc gtacgtcctg atcgggttcg agcgggaaaa ggccgaggtg cgcgcctccg
     3541 ccctccaggc gctcctcgtc accgggggcg gcggcctcgc gctcctcgcc ggcttcgtcc
     3601 tcctcgggca ggcgggcggg agcctcgaga tcggcgctct cgcggcgaat gcggaggcgg
     3661 tgaaggcgag ccccctctac ctcccggcgc tcctcctcgt cctcctcggc gcctttacga
     3721 agtccgcgca ggtgccgttc cacttctggc tccccgacgc gatggccgcg cccgctccgg
     3781 tgagcacctt cctccactcc gcgacgatgg tgaaggcggg cgtcttcctc ctcgcgcgcc
     3841 tccacccgac gctcggcggg acgccggagt ggaccgggat cgtgacgacc gtcggcgcgg
     3901 cgacgatgct gacgggggcc ctcctcgccg tggggcagcg ggacctcaag cggctcctcg
     3961 cctactccac cgtcagcgcc ctcggctcgc tcgtcatgct cgtcggcgtc gggacgccgg
     4021 cggccctcgc cgcggcgatg gtcctcctcc tcgcgcacgc gctttacaag ggagcgctct
     4081 tcctcgtcgc ggggatcgtc gaccacgaga ccgggacgcg cgacgtcgcg agcctcggcg
     4141 gcctcttccg ggccctgccg gtgacggcgg tcgccgcggc ggccgcggcc ttctcgatgg
     4201 cgggactccc gccgctcctc ggcttcgtca gcaaggagct cctctacgac gcggccctcc
     4261 gcgcctcgcc cgctccgctc ctcctcgtcc cggtggcggt cggctcgaac atgctcctcg
     4321 tggccgtcgc cctttccgtc ggatgggggc cgttcacggg ccgggcgggg gagacgccga
     4381 aggcccccca cgaggcgccc gccgccatgt gggccggccc gctcctcctc ggcctcggaa
     4441 gcctcgccgc cggtctcttc tcgggggcga tctcgccgtt cgtctcttcc gccgccgccg
     4501 ccgcgctcgg cgcgccgcac gccctcacgc tggagctctg gcacggcttc aacctcgtcc
     4561 tcgccaagag cctcgccacg ctcgtcgtcg gcgtcgccct cttcgcggcg cggggccgaa
     4621 tccgttccct cgcggggagc ctcgccccgc tcgccgcgtt cggtcccggc cgcctctacc
     4681 gcgcgggcct cgccggcctc gtccggctcg cgaaggggac ggcggcgctc ctctcgtccg
     4741 gctcgctccg gcaggacctg cggatcgtcc tcgccgcgac ggtcgccctc gccgccccgc
     4801 tcctcctgac gcgggccgtc ctcggggcgc ccgacgccct ccggtcggtc cggccgctcg
     4861 aggcggtcct cgcgctcctc gtcgtcgccg gagccgtcgc caccctcctg gccggctcgc
     4921 gcctcgcggc cgtcgtcgcg ctcggcgtga cgggcttcgg cgtcgccctc ctcttcctcg
     4981 tcttcggcgc ccccgacctc gcgatgacgc agttcgcggt cgagacgctc tccgtcctcc
     5041 tcttcgtcgc ggtcctgcgg cgcctcccga ggctccagag ccggtcggcc ccgcgcgcgc
     5101 gggtcgtcga cgccctcctc gccggcgcgg tcggcgccgt catgaccggg ctcgtcctgg
     5161 cggtccacgc cgagccgctt cgctcgagcc tcgccccctt cttccgggaa tcgagcctcg
     5221 ccctcgcgaa ggggcggaac gtcgtcaacg tcatcctcgt cgacttccgc ggcctcgaca
     5281 cgctcggcga gatcacggtc ctcggcgtcg ccgcgctcgg cgtcttctcg ctcgtgcggc
     5341 tccggatcgc gtcccgcgcc gccggcgcga ggggggaccg gtcgtgaggt cggtcatcct
     5401 cgccgccgcg acgcgggtcg tcttcccgct cctcctcctc ttctccgtct tcctcctctt
     5461 ccgcgggcac aacgagcccg gcggcggctt cgtcggcggc ctcgtcgcgg cgacggccta
     5521 cgccctcctc gcgctcagcg gaggggtcgc ttcggcgcgg cgcgtcctcc cggctcgccc
     5581 ggaggtcctg atcggcacgg gtctcctggc cgcgctcgcg agcggcctcg tcggcctcgc
     5641 ggcgggccgg ccgttcatga cgggtctctg gggcgacgtc tccctgccgg tcgtcgggaa
     5701 gccgggaacg ccggtcctct tcgacgcggg ggtctacctg acggtcctcg gcgtcgtcct
     5761 cctgattctc ttcacgctcc aggaggagga cgaggcgtga cgctcctcct cgcgctcgcg
     5821 atcggcgccc tctacgccgc ggggacctac ctcctcctgc ggcggagcgc gatccgcgtc
     5881 gtcttcggcc tcgcccttct cggctacgcg tcgaacctcc tcgtcttcac cgcgggacgc
     5941 ctctcgcggg agggccctcc gatcgtcccg gagggggccg cgacggcggc cgccacgtcg
     6001 gccgatcctc tcccgcaggc gctcgtcctg acggcgatcg tcatcgggtt cggcgtccag
     6061 gcgttcgccc tcgtcctcct gaagcggacc tgccaggagg cggggaccga cgacgtcgac
     6121 gcgatgcggg aggagacatg aacctcctcc tcgtcgtccc gctcctcgtc ccgctcgcga
     6181 ccgcggccgc gacgatcctc ctcgcgcggt cgccgcgggc cgtgcgcgcg gcgagcttcg
     6241 ccggggcggc ggcgttcctc gccgcggcgg tcgcgctcct cggcgcgacg ctcgacggga
     6301 cgatcctcgt cacgcaggcc ggggcctggg cggcgccgtt cgggatctcc ctcgtcgccg
     6361 accgcttcgc ggccgcgatg gtcctcgtgg ccgccgtcct cctcgcggcg gtcgtcgcct
     6421 attcggtccg aggcctcgac ggggcgcgcg agcgggcggg ctaccacccg ctcctccacg
     6481 tcctctcgat gggcgtctcc ggggccttcc tgacgggcga cctcttcaac ctctacgtct
     6541 ggttcgaggt catgctgatg gggtcgttcg tcctcgtctc cctcgggggc gagcgggcgc
     6601 agctgcaggg ggccctgaag tacgtcgtcc tgaacctcgt ctcctcggcg ctcttcctgg
     6661 cgggcgcggg gctcctctac ggttccgtcg ggacgctcaa catggccgac gcggccgtcg
     6721 cgctccgcga gctgccggcg caggggctcc cgagcgtcgt ggcctccctc ttcctcctcg
     6781 ccttcgggat caaggcggcc gcgttccccc tcttcttctg gctcccggcc tcgtaccaca
     6841 cgccgccgat gccggtggcg gcgctcttcg cggggctcct gacgaaggtc ggcgtctacg
     6901 ccctcgtgcg cgtcctcacg ctcgtcttcg tccgcgacac ggccttcacc ggcccgctcc
     6961 tcctcgccgt cgcggcgctc acgatggtca ccggcgtcct cggcgcgatg gcgcagggcg
     7021 acgtgcggaa gatcctctcc ttccacatcg tcagccagat cggctacatg ctgatggggc
     7081 tcggcctcgg gacgcggctc gcgctcgccg ggacggtctt ctacgtcctc caccacatcg
     7141 tcgtgaaggc gaacctcttc ctcgtcgcgg gagcgatgcg gcgggccggg gggagcttct
     7201 cgctcgatcg cctcggctcc ctcctcgccc gctcgcccct cctcgccgcg gcgttcctcg
     7261 tcccggcggt ctcactcgcg ggactcccgc ccctctcggg gttctgggcg aagtacctcc
     7321 tcgtgagggc cggcctcgag gcggggaacg ggacgatcgt cgccgtggcg ctcgccgtca
     7381 gcctgctgac gctcttctcg atgacgaaga tctggaacga ggcgttctgg aagccggccc
     7441 cggccggagc cgacgcggcg cccgcgccgc tgacgacggc agagcgggcc tcgctcctcg
     7501 ggccggtcct cgtcctcggc gcggtcacgg tcgcgatcgg cctcggagcg ggggtcctct
     7561 atcgcttcgc ggacgcggcg gccgcccagc tcctcgaccc ggacctctac gtccgcgccg
     7621 tcctcggcgc gcgcgacggg aggatgtgac cggtgggcac gttcgcgttc ctcgtcttcg
     7681 cctgggtcgc cgtcaccgga gaggtcacgg tcgggaacct cctcgaaggg gcggtcctcg
     7741 ccggcctcct cgtcctcctc ctccggatcc ccgtgcgccg ccgggtccgt ctcgagcagg
     7801 tgccgaaggc gctcggtctc ctcctctact tcctgaagga gatcgtcctc tcgaacgcgg
     7861 cggtcgcccg gagcctcctg tcgcccgtct cgtccctctc gcccggcatc gtcgccgtcc
     7921 cgctcgacct gaagagcgac gccgggatca ccgtcctcgc gaacctggtc acgctcactc
     7981 ccgggacgct gagcctcgac gtctcgcccg accggacgac gctctacgtc cacgccctcc
     8041 acgtcgagga cccggaggcc ttccggcggg aggtgaagga aggattcgag aggcgcgtaa
     8101 aggaggtctt cgagtgacgc tcctctcgca gctcgcgctc tgggtcggcc ttcc
//
LOCUS       DAOTEZ010000173         8053 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_6326_length_8053_cov_6.065016, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000173 DAOTEZ010000000
VERSION     DAOTEZ010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8053
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6326_length_8053_cov_6.065016"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1845)
                     /gene="aspS"
                     /locus_tag="P5164_10360"
     CDS             complement(64..1845)
                     /gene="aspS"
                     /locus_tag="P5164_10360"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRY44338.1"
                     /translation="MSATVPRVPAGSLRREDAGRTVQVKGWVHRRRDFGELVFVDLRD
                     RSGILQVVFDAELLDEPELLGRAKELRGEYVLAVTGAIVPREGSARNPDMPTGDVELR
                     ATALELLNRAETPPFVIEDDVRASEDLRLKYRYLDLRRPEMTSNLVKRSDVTFRIRQV
                     LHEEGFLEVETPILTKSTPEGARDFLVPSRVHPGEFYALPQSPQLFKQLLMVAGMDRY
                     YQIARCFRDEDLRADRQPEFTQVDLELSFPDEETVYALIEKLFAAVFPAFGIPCPVPF
                     PRMTHAEAMDRFGIDRPDTRFGLELRALDVSASPSEILRSAETAKGIAVPGGAAFSRK
                     RLDELEAAAKQLGAAGLVWVKLQAEPPGSWGSNAKKMLDDATVASARAALEAKDGDLL
                     LVVAGKRATVNDVLGALRLRIAREEKLVPEDRFDFLWVTDFPLLEWHPEDGRWYATHH
                     PFTAPRPEDVPFLDTDPGRVHARAYDVVLNGMELGGGSIRIHQPEVQSKVFATLGISP
                     DEAKAKFGFLLEAFRYGAPPHGGLALGLDRMLMVLTRSASIRDVIAFPKTASASCLMT
                     ESPGPVADAQLAELGIRRAGDDGPKPA"
     gene            complement(1842..3101)
                     /gene="hisS"
                     /locus_tag="P5164_10365"
     CDS             complement(1842..3101)
                     /gene="hisS"
                     /locus_tag="P5164_10365"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRY44339.1"
                     /translation="MITSVKGMRDLLPPDTRVWNRLEETARNVFSLYGYDEIRTPVLE
                     PTELFVRSVGEASDIVHKEMYAFTDRGGRDVSLRPENTAGVARAYVEHQMPQAGGVKK
                     LYYVGPQYRYERPQKGRYREFRQIGAEVLGAADPATDAELLVMLFDLFTRLGFTGLSV
                     SLNSVGSEESRPAYVEALRAYLAAHEGTMGEDDRRRLRDNPLRVLDTKDPALAAALAG
                     APRVLDHLDPESKAHHEELLALLDAAGIPYRVEPRLVRGLDYYTRTVFEVTAQGLGAQ
                     DALLGGGRYDRLVSDLGGPRTPGIGFAIGEDRLVDVLPEPFRRAAIEGFAPVAVVAID
                     PADRAAALALAGELRSAGVAVDLDPAGKGPGHALKNAAKRGCRTAVLVGEKERQSGVV
                     VVKDLVERSQREVPIGDLPRTLKGETP"
     gene            complement(3118..3675)
                     /locus_tag="P5164_10370"
     CDS             complement(3118..3675)
                     /locus_tag="P5164_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRY44340.1"
                     /translation="MIIRPFRGATPALGERVFLAETAAVVGDVVLGDDVSIWYGSVVR
                     GDCCSIRIGARSNVQDNSTLHVTKDVGPLLLEEEVTLGHNCVVHACTIRRGALVGMNA
                     TVLDGAEIGEKALVAAGAVVLGGTVVPPRTLWAGSPARLRRPLTDAEADGLDAYWRNY
                     LEYKEEYLREDARRGRSLPFPVPVL"
     gene            complement(4004..5776)
                     /gene="recN"
                     /locus_tag="P5164_10375"
     CDS             complement(4004..5776)
                     /gene="recN"
                     /locus_tag="P5164_10375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRY44341.1"
                     /translation="MLSSLRVRDLAVLEDVDVALEPGLTVLTGETGAGKSLVVDALSL
                     LSGERSDATLVRGGAERLVVEGSFETDDPAVGAALSAAGLRDGEARGPSELVVRREVS
                     SAGKGRLFVDGSPAALRTLADVAPRLLVLYGQAEARELLDPAAPRELLDRFAALTERA
                     AEVARLHAAFREKEADRERIAALGKDGPARLEVLDFRIGEIDRAAPVAGEEEALAREK
                     VSLQSVEKRGRLLADAVTALESEEGGALPALTALRRTFAALAELDASAAERLAAVDDL
                     LERVADLAASTSRELERLEADPARLGEVVERLDLLARLKRKYGATLEEVLSYRDAIGR
                     ERDELADLEGALRKAEKASAEAFAAFRAAALDLSSERQAAAPKLAKAVERHLSDLAFL
                     SSSFRVEVSRRAEPDGAFLAGEDRVAFGPHGVDVVSLAFAPNPGEPPRPLAKIASGGE
                     LSRVQLAVAAALAEAETAREGRRGSRRGGPVRTFVFDEVDAGVSGATAEAVGRKLRAL
                     AARDQVLVVTHLAQVAAAGERHLAVRKEVAAGRTRTVVTSLDEKGRVEAIAALLAGAS
                     VTDAARRQARALLASAGDGPRGTR"
     gene            complement(5781..7292)
                     /locus_tag="P5164_10380"
     CDS             complement(5781..7292)
                     /locus_tag="P5164_10380"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HRY44342.1"
                     /translation="MKLEIVRPDEKRTGGRRDAAVAFLARPEKKGPARFESLPEGFRD
                     AVRAAAPPDAGNDGSLFTLPLGPDAPARRLYVLGTGTGDELSPRKARVLLRGAVKALE
                     KNGERTALVDFPFTLQRMTAAEARDFVVRSLLVAGYVFPRYKASAREAARLSALALSA
                     GRGVFAATPKELKAASDEASIVAAASAFVRDLGNTPGSDMVPRTFADAVKAEARKRGV
                     KATVLGKKEIERERMGGLLAVNRGAAEEPRFVILEHRGGRKGEKPVVLVGKGVTFDSG
                     GISLKPADRMGDMKWDMLGAATVCGAVLAAAGLGLPVNVVALATLTENMPSGTAYKPG
                     DIVTFRNGKTAEIDNTDAEGRVILADALDYAMGLSPAAVVDFATLTGAALVALGLEAS
                     IVFSDHDDLVRELVSAGERTDERLWRLPLWDDYKENIRSDWADFKNTGGRNGGSINGA
                     MFLKEFVDPKTPWAHVDIAPNAYFEREHAGYPPGATSIGLAMTLRWLRDRAAR"
     gene            7354..>8053
                     /locus_tag="P5164_10385"
     CDS             7354..>8053
                     /locus_tag="P5164_10385"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRY44343.1"
                     /translation="MPPAALDATALPGLPPPRRGKVRDVYDLGERLLIVATDRISAFD
                     VVLSPGIPGKGAVLTQLSTFWFRKFEALVPNHLLETDAAAFPAELAGFAGLLAGRSVL
                     ARKCRVVPFECVARGWLAGSGWKDYRRSGAVCGVPLPAGLSEASRLPEPIFTPATKAE
                     EGHDENVSFEAMANAVGRDLASRLRDLTLAIYTEAASYAAGRGVIVADTKLEFGLDDA
                     GSVVWIDEALTPDSS"
BASE COUNT         1066 a         3013 c         2882 g         1092 t
ORIGIN      
        1 ccgaccgcca cccggcacgg aacgccggcc gccacgaagc ggaaggcgcg gacggcgccc
       61 ggctcaggcc ggcttcggac cgtcatcccc cgcccggcgg atgccgagct cggcgagctg
      121 ggcgtccgcg accggcccgg gcgactccgt catgagacag ctcgccgagg ccgtcttcgg
      181 gaacgcgatc acgtcccgga tcgaggcgga gcgcgtcagg accatcaaca tccggtcgag
      241 cccgagggcg aggccgccgt gcggcggcgc gccgtagcgg aacgcctcga ggaggaagcc
      301 gaacttcgcc ttcgcctcat cgggagagat gccgagcgtc gcgaagacct tcgactggac
      361 ctccggctgg tggatccgga tcgacccgcc gccgagctcc atgccgttca ggacgacgtc
      421 gtaggccctc gcgtggacgc gccccgggtc cgtgtcgagg aagggcacgt cctcgggccg
      481 cggcgcggtg aacgggtggt gcgtcgcgta ccagcggccg tcctccgggt gccactcgag
      541 gagcgggaaa tcggtgaccc agaggaagtc gaaacggtcc tccgggacga gcttctcctc
      601 gcgcgcgatc cggagccgga gcgcgccgag gacgtcgttc accgtcgccc tcttcccggc
      661 gacgacgagg aggaggtcgc cgtccttcgc ctcgagcgcg gcccgggccg acgccacggt
      721 cgcgtcgtcg agcatcttct tcgcgttcga gccccagctc ccgggcggct ccgcctggag
      781 cttcacccag acgagcccgg ccgccccgag ctgcttcgcc gcggcctcga gctcgtcgag
      841 ccgcttgcgt gagaaggccg ctccgcccgg cacggcgatc cccttcgccg tctcggccga
      901 ccggaggatc tccgagggtg aagcggagac gtcgagcgcg cggagctcga ggccgaatcg
      961 cgtgtcgggc cggtcgatcc cgaaccggtc catcgcctcg gcgtgcgtca tccgcgggaa
     1021 cgggacgggg cacgggatgc cgaaggccgg gaagaccgcc gcgaagagct tctcgatcag
     1081 cgcgtagacc gtctcctcgt cggggaacga cagctcgagg tcgacctgcg tgaactcggg
     1141 ctgccggtcg gcccggaggt cctcgtcgcg gaagcagcgc gcgatctggt agtagcgatc
     1201 catccccgcg accatcagga gctgcttgaa gagctgcggg gactgcggga gcgcgtagaa
     1261 ctctccgggg tggacgcgcg acgggacgag gaagtcgcga gccccctcgg gcgtcgactt
     1321 cgtcaggatc ggcgtctcca cctcgaggaa cccctcctcg tggaggacct gccggatccg
     1381 gaacgtcacg tcgctccgct tcacgaggtt cgacgtcatc tccggccggc ggaggtcgag
     1441 atagcggtac ttcagccgga ggtcttccga ggcccggacg tcgtcctcga tcacgaaggg
     1501 cggcgtctcg gcgcggttga ggagctcgag cgccgtggcc cggagctcga cgtcccccgt
     1561 cggcatgtcg ggattccggg cggagccctc cctcgggacg atcgcgccgg tcacggcgag
     1621 gacgtactcc ccgcgcagct ccttggcgcg cccgaggagc tccggctcgt cgaggagctc
     1681 ggcgtcgaag acgacctgca ggatccccga gcggtcccgc aggtcgacga agacgagctc
     1741 gccgaaatcg cggcggcggt ggacccagcc cttgacctgg accgtccgtc cggcgtcctc
     1801 cctgcggagc gagccggcgg ggacgcgggg cacggttgcg ctcacggcgt ctctcccttc
     1861 agcgtgcgcg ggaggtcgcc gatcggcacc tccctctgcg acctctccac gaggtccttg
     1921 acgacgacga cgcccgactg ccgctccttc tcacccacga ggaccgccgt gcggcagccg
     1981 cgcttcgcgg cgttcttcag cgcgtgaccc ggtcccttcc cggccgggtc gaggtcgacg
     2041 gcgacgcccg cgctccggag ctcgccggcg agcgcgaggg cggcggcccg gtcggcggga
     2101 tcgatcgcca cgaccgcgac cggggcgaag ccctcgatcg ccgcgcggcg gaaaggctcc
     2161 ggcaggacgt cgacgagccg gtcctctccg atcgcgaagc cgatgcccgg cgtcctcggc
     2221 ccgccgaggt ccgacacgag ccggtcgtac cgccctccgc cgagcagcgc gtcctgcgcg
     2281 ccgaggccct gcgccgtcac ctcgaagacc gtgcgcgtgt agtagtcgag accccggacg
     2341 agccgcggct cgacccgata ggggatcccc gccgcgtcga ggagcgcgag gagctcctcg
     2401 tggtgcgcct tcgactccgg gtcgaggtgg tcgaggacgc gcggcgctcc ggcgagcgcc
     2461 gcggcgagcg cggggtcctt cgtgtcgagg acgcggagcg ggttgtcgcg cagccgccgc
     2521 cggtcgtcct cccccatcgt cccctcgtgc gcggcgaggt aggcgcgcag cgcctcgacg
     2581 taggcggggc gcgactcctc gctcccgacg ctgttcagcg agaccgagag gcccgtgaac
     2641 cccagtcggg tgaagaggtc gaagagcatc acgaggagct ccgcgtcggt cgcggggtcg
     2701 gccgcgccga gcacctcggc tccgatctgc cggaactcgc ggtagcgccc cttctgcggc
     2761 cgctcgtagc ggtactgcgg accgacgtag tagagcttct tcacgccgcc cgcctgcggc
     2821 atctggtgct cgacgtaggc gcgcgccacg cccgcggtgt tctcgggccg gagcgagacg
     2881 tcgcgcccgc cccggtcggt gaaggcgtac atctccttgt ggacgatgtc cgacgcctct
     2941 cccacgctcc gcacgaagag ctccgtcggc tccagcacgg gcgtccggat ctcgtcgtag
     3001 ccgtagaggg agaagacgtt ccgggccgtc tcctcgaggc ggttccagac gcgcgtatcg
     3061 ggcgggagca ggtcccgcat tcccttgacg ctcgtgatca tcggggccgg cccttcgtca
     3121 gaggacgggc acggggaagg gaaggctccg cccgcgccgc gcgtcctctc ggaggtactc
     3181 ctccttgtat tccaggtagt tccgccagta cgcgtcgagc ccgtcggcct ccgcgtcggt
     3241 gagcggacgg cggagccgcg ccggactccc cgcccagagg gtccgcggag ggacgacggt
     3301 gccgccgagg acgaccgcgc ccgcggcgac gagcgccttc tcgccgatct cggccccgtc
     3361 caggacggtc gcgttcatcc cgacgagcgc cccgcgccgg atcgtgcagg catggacgac
     3421 gcagttgtga ccgagcgtca cctcctcctc gaggaggagc ggtccgacgt ccttcgtgac
     3481 gtgcagcgtg gaattgtcct ggacgttcga gcgcgcgccg atacggatcg agcagcagtc
     3541 gccgcggacc accgagccgt accagatcga gacgtcgtcc ccgaggacga cgtctccgac
     3601 gacggccgcc gtctcggcga ggaagaccct ctcgccgagg gccggggtcg cgccgcgaaa
     3661 gggccggatg atcacgtcgt cctcacgggc cgccgacgat accagaagcc gaagcgccgc
     3721 ttcgagacga gcgcctcgcg cgattgcgct ctccgctccc gtcgatccgc ccgggtgcac
     3781 cgaaacgtgt gcacggtgga agaacgtcgg gaacgacggg gggttcgggc ccctcctcgt
     3841 gagttacgca cagggttttc ctcagaccct ctgaattcct tcgcgtcgtc ttcatctcga
     3901 tcagcagccg atgaacacgt gcccgtcccg aaacgggacg ggcacgccgc gtgcacggag
     3961 aggcgcaccc gaactggccc ttgacaagcc tcgtgcgtcg cgctcagcgc gtgccgcgcg
     4021 ggccgtctcc cgcggacgcg aggagcgcgc gcgcctgccg ccgcgccgcg tcggtcaccg
     4081 acgcgcccgc gaggagcgcg gcgatcgcct cgacgcgccc cttctcgtcg aggctcgtca
     4141 cgacggtgcg cgtccgcccc gccgcgacct ccttgcgcac ggcgaggtgg cgctccccgg
     4201 cggccgccac ctgcgcgagg tgcgtgacga cgaggacctg gtcgcgcgcg gcgagggccc
     4261 ggagcttcct cccgaccgcc tcggccgtcg ctcccgacac gcccgcgtcg acctcgtcga
     4321 agacgaacgt cctcaccggg ccgcctcgcc ggctcccgcg ccgcccctcg cgcgcggtct
     4381 cggcttcggc gagcgcggcc gcgacggcga gctgcacccg ggaaagctcg cctccggacg
     4441 cgatcttcgc gaggggcctc ggcggctctc cggggttcgg cgcgaaggcg agcgacacga
     4501 cgtcgacgcc gtggggaccg aacgcgacgc ggtcctcgcc cgcgaggaaa gccccgtcgg
     4561 gctcggcccg gcgcgagacc tcgacccgga aagacgagga gaggaacgcg aggtcggaga
     4621 ggtgcctctc cacggccttc gcgagcttcg gcgccgcggc ctgccgctcg gaggagaggt
     4681 cgagcgcggc ggcgcggaac gcggcgaacg cctcggccga cgccttctcc gctttccgga
     4741 gcgcgccttc gaggtcggcc agctcgtccc gctcgcgtcc gatcgcgtcg cggtaggaga
     4801 ggacctcctc gagcgtcgcc ccgtacttcc gcttcagccg cgcgaggaga tcgagcctct
     4861 cgacgacctc gccgagcctc gcggggtcgg cctcgaggcg ctcgagctcc cgggaggtcg
     4921 acgccgcgag gtcggcgaca cgctcgagga ggtcgtccac ggcggcgagc cgctccgcgg
     4981 cggaggcgtc gagctcggcg agcgcggcga acgtccgacg gagggccgtc agcgcgggga
     5041 gcgcgccccc ctcttccgac tcgagcgccg ttacggcgtc ggcgaggagg cgtccgcgct
     5101 tctccacgct ctggagagag accttctctc gcgcgagcgc ctcttcctcg cctgcgacgg
     5161 gcgccgcccg gtcgatctcg ccgatccgga agtcgagcac ctcgaggcgc gccggcccgt
     5221 ccttcccgag cgcggcgatc cgctcgcggt cggcctcctt ctcccggaac gcggcgtgga
     5281 gccgcgccac ctccgcggcc cgctccgtga gcgccgcgaa gcggtcgagg agctcccgcg
     5341 gcgcggccgg gtcgaggagc tcccgcgcct ccgcctgccc gtagaggacg aggagccgcg
     5401 gcgccacgtc ggcgagcgtt cggagcgccg cgggagagcc gtcgacgaag aggcgcccct
     5461 tccctgccga ggagacctcg cggcgcacga cgagctccga cggtccgcgc gcttccccgt
     5521 cccggagccc cgcggcggag agcgccgcgc cgaccgccgg gtcgtcggtc tcgaaggagc
     5581 cctccacgac gagccgctcc gccccgccgc ggacgagcgt cgcgtccgag cgctcgcccg
     5641 acaggagcga gagcgcgtcg acgacgagcg acttgccggc gcccgtctcc cccgtcagga
     5701 cggtcaggcc cggctcgagc gcgacgtcga cgtcctcgag gacggccagg tcgcggacgc
     5761 ggagcgacga gagcacggcc tcagcgtgcg gcgcggtccc ggagccagcg aagcgtcatc
     5821 gcgaggccga tcgacgtggc gccgggcggg tagccggcgt gctcccgctc gaagtaggcg
     5881 ttcggcgcga tgtcgacgtg cgcccagggc gtcttcgggt cgacgaactc cttgaggaac
     5941 atcgccccgt tgatcgaccc gccgttccgc cctcccgtgt tcttgaagtc ggcccagtcg
     6001 gaacggatgt tctccttgta gtcgtcccag agcggcaggc gccagaggcg ctcgtccgtc
     6061 cgctcgccgg ccgagacgag ctccctcacg aggtcgtcgt ggtcgctgaa gacgatcgag
     6121 gcctcgagcc cgagggcgac gagggccgcg ccggtgagcg tggcgaagtc gacgaccgcc
     6181 gcgggcgaga gccccatcgc gtagtcgagc gcgtccgcga ggatgacgcg cccctcggcg
     6241 tcggtgttgt cgatctccgc cgtcttcccg ttccggaagg tgacgatgtc gcccggcttg
     6301 taggccgtcc ccgagggcat gttctcggtg agcgtcgcca gcgcgacgac gttcaccgga
     6361 agcccgaggc cggccgccgc gaggaccgcg ccgcagaccg tcgccgcgcc gagcatgtcc
     6421 cacttcatgt cgcccatccg gtcggcgggc ttgagggaga tgcccccgga gtcgaacgtc
     6481 acccccttgc cgacgaggac gaccggcttc tccccctttc gcccgccgcg gtgctcgagg
     6541 atcacgaagc gcggctcctc ggcggcgccg cgattgacgg cgaggaggcc gcccatccgc
     6601 tcgcgctcga tctccttctt cccgaggacg gtcgccttca cgccgcgctt tcgcgcctcg
     6661 gccttcacgg cgtcggcaaa cgtccgcggg accatgtcgc tgccgggggt gttcccgagg
     6721 tcgcggacga aggccgacgc cgccgccacg atcgacgcct cgtcggacgc cgccttcagc
     6781 tccttcggcg tcgccgcgaa gacgccccgc cccgcggaga gggcgagcgc ggagagtcgc
     6841 gccgcctccc gggccgaggc cttgtaccgg gggaagacgt agccggcgac gaggaggctc
     6901 cggacgacga agtcgcgcgc ctcggccgcg gtcatccgct ggagggtgaa cggaaaatcg
     6961 accagcgcgg tccgctcgcc gttcttctcg agcgccttca cggcgccccg cagcaggacg
     7021 cgcgccttgc gcggcgagag ctcgtcgccg gtccccgtgc cgaggacgta gaggcgccgc
     7081 gccggggcgt ccggcccgag cgggagcgtg aagagcgacc cgtcgttccc ggcgtcggga
     7141 ggcgcagccg cccggaccgc gtcccggaac ccctcgggca ggctttcgaa gcgcgcgggc
     7201 cccttcttct cgggtcgcgc caggaaggcg acggcggcgt cccgccggcc gcccgttctc
     7261 ttctcgtcgg gtcgaacgat ctcgagcttc acgcgtgtct cctccgcggg ctgcggcgga
     7321 gggagtctag ccgaggccga atagaatccg cccatgccgc ccgccgccct cgacgcgacc
     7381 gccctccccg gcctgccccc gccgcggcgc gggaaggtcc gcgacgtcta cgacctcggc
     7441 gagcggctcc tgatcgtcgc cacggaccgg atctccgcct tcgacgtcgt cctcagcccc
     7501 ggcatccccg gcaagggggc cgtcctcacc cagctgtcga ccttctggtt ccggaagttc
     7561 gaggcgctcg tgccgaacca cctcctcgag acggacgcgg ccgcgttccc cgccgagctc
     7621 gccgggttcg cgggcctcct cgcggggcgc tccgtcctcg cgcgcaagtg ccgggtcgtc
     7681 ccgttcgagt gcgtcgcccg cggctggctc gccggctcgg gctggaagga ctaccggcgg
     7741 agcggcgccg tctgcggcgt gccgctgccg gccggcctct ccgaggcgtc gcggctcccc
     7801 gagccgatct tcacccccgc gacgaaggcg gaggaagggc acgacgagaa cgtctccttc
     7861 gaggcgatgg cgaacgccgt cgggcgcgac ctcgcgtcgc gcctgcgcga cctgacgctc
     7921 gcgatctaca ccgaagcggc ctcgtacgcc gccgggcgcg gcgtcatcgt cgccgacacg
     7981 aagctcgagt tcggcctcga cgatgccggc tccgtcgtct ggatcgacga ggcgctcacg
     8041 cccgactcct ccc
//
LOCUS       DAOTEZ010000174         8042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_6341_length_8042_cov_5.695881, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000174 DAOTEZ010000000
VERSION     DAOTEZ010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8042
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6341_length_8042_cov_5.695881"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="P5164_10390"
     CDS             complement(<1..548)
                     /locus_tag="P5164_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxyltransferase"
                     /protein_id="HRY44344.1"
                     /translation="MKTWNLPKRVDLADITVRDGFQHEERWIPTEAKLWVLEELILAG
                     YRRLEVTNLGNPKGMPQFRDADELLKAIRGSARVAKLIGETELTAVTIREKAVDRAIA
                     AKAEGWGPDRILMMVSTSASHMKKNSGLDHAGYWAEAERCIRKAHDAGIRVNGTVSTI
                     WGCPIEGPTRLEDAVTFTKRFL"
     gene            complement(551..883)
                     /locus_tag="P5164_10395"
     CDS             complement(551..883)
                     /locus_tag="P5164_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44345.1"
                     /translation="MNHDSLVKDAAARTGLPEPEVEKVIAALADVVHHAHATPDELLH
                     ALLGASAPLHAHPVDPRDAAVVAELVERAKVHPLGLDYLKGGHLGSVAVTFETHAFTV
                     LAARELLR"
     gene            complement(880..1923)
                     /locus_tag="P5164_10400"
     CDS             complement(880..1923)
                     /locus_tag="P5164_10400"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HRY44346.1"
                     /translation="MKRASRLAAAAAVLTLAACSRSAPEPRIGHEERKALAAQASTTL
                     GVLPEKMPGADSDSPQLVALGRSLWFERKLSVTGTQSCNDCHRLDGEHPTGADGEATS
                     DGAKGTKGPRNSPSVKNAGYHVAQFWDGRAKTLEEQAEGPILNPIEMAMPDAAAVEAA
                     LRGAASYGEPFAAAFPGEAEPITLKNTARALAAFERTLKTRDRLDDFLRGDLDALSNR
                     EAAGLKLFLATGCTTCHNSPTIGANQFQVLGLVRPWDTDDEGRFAVTGNEEDRRKFKV
                     PSLRNAVATGPYFHDGSVDRLETAVKKMAWHQLGKELTDDEIASIVAFLGALADRSGP
                     VLPPTAPTKETAR"
     gene            2007..3155
                     /locus_tag="P5164_10405"
     CDS             2007..3155
                     /locus_tag="P5164_10405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY44347.1"
                     /translation="MRSPWFRRGGVLLGIVAAVLLLRATLFRPDPVPVTVVAAAKGTV
                     EETVAGSRAGTMRSRRRATLSPEVGGRVERLAVREGDRVKKGDLLVRIAADDVKAQVA
                     LAEKSLAVAQAAEVEACRAAEQARRDLARSRSLAKDEVLSVGLLEKAETEAGMTGAAC
                     EAARARVGQARAALDVARIALAKTVLKAPFDGVVAEVSTEEGEWVTPSPPGLPIPPVV
                     ELFDPDDVYVSAPLDEVDVGRVKKGAEVRVTFDAYPGRTFAGRVTRVADYVLDQREQN
                     RTFEVEVALDDSSFARTLLPGTSADAVVVLARKEEVLRVPAYAILPGGRILVLSKGRL
                     AGAKVETGLKGAEWAEVVSGLSAGDLVVTSLDRPEVKEGARARAEEAR"
     gene            3152..3826
                     /locus_tag="P5164_10410"
     CDS             3152..3826
                     /locus_tag="P5164_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY44348.1"
                     /translation="MIELSGVSRTWEVGGRPVHALRDVTLSIGSGEYVSVMGPSGSGK
                     STLLSLLGGLDRPTAGSYRFEGREVASLPDDDLSRLRRERIGFVFQSFHLVPRMTALE
                     NVALPLLLAGVSPKDRTARAAEALASVGLSPRLDHRPDQLSGGEKQRVCLARAVVTRP
                     GLLLADEPTGNLDSASGTEIVALLERLHASGITLLVVTHDAGIGGRARRRVRLADGRV
                     TGDEAA"
     gene            3823..5025
                     /locus_tag="P5164_10415"
     CDS             3823..5025
                     /locus_tag="P5164_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY44349.1"
                     /translation="MRGGDLLAFAAGAFRGHKLRTSLSLLGVAIGVAAVILLTSLGEG
                     ARRYVTGEFALLGSNLVIVLPGKSETTGVVPVGGVPHDLTLEDVEALRRRVSLLVSVA
                     PLSVGGLTARFGERSRDLTIAGVTSEWKDVRRLALREGAFIPPGDPDRAPRVCVVGAK
                     VASELFPGRSPVGELLRLGEERFRVTGVLVPRGVSVGLDLDEVVLVPIGHHLRMFDRR
                     SVFRVLCEARTSEDLEAAKAGILAVLKDRHDGEEDVTVLTQDAVMKTFGKVLGILTAA
                     LAGIAAVSLTVAGVGIMNVMLVSVSERTREVGLLKALGASGGQVLRLFLVEAALLSSA
                     GGALGLLAGWAGTLAIRALYPDFPVAPPAWAVAAALAVSVSVGLVFGVLPARRAARLD
                     PVVALARR"
     gene            5030..6232
                     /locus_tag="P5164_10420"
     CDS             5030..6232
                     /locus_tag="P5164_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY44350.1"
                     /translation="MAFADVLRLALGSVTAHRLRSALTVLGIVIGIASVVLLTSLGEG
                     TRQAILSEFSQFGTNLLAVTSGKIETHGIAGAVGGTTRPLTLDDAEALRAVPGIEKVV
                     PVVFGTARVSSGERARDTFVYGATSDGPEVWRFQVRLGRFLPETELDRATPVAVLGPK
                     LKRELFGGENAIGRRIRVGGHRFTVIGVMATVGQFVGFTLDDAVYVPVASAQQLFDQA
                     QVMEIDATFTPGLPPETVVAAVKRVLKARHGGEEDFTVLTQGEMLESFGRVFAVVSVA
                     VGAIGAISLLVGAIGILTMMWIAVGERTAEIGLLKALGATRGDVRNVFLAEAAILSGA
                     GGLAGIAAAMSLGAIVRLALPAVPFATPPEYAAAALATAVGVGLVAGVAPARRAAALD
                     PVENLRAE"
     gene            6238..6771
                     /locus_tag="P5164_10425"
     CDS             6238..6771
                     /locus_tag="P5164_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRY44351.1"
                     /translation="MSDAVPASWQEEVFSALREAGVRQVAWVPDAGHAHVIDRVLVDP
                     EMLGIPLTTEEEGVALSCGAWLGGDRAVLLMQSSGVGNCVNMLSVVSTCRFPFLSLVT
                     MRGEWEERNEWQEPMGRATGTVLEAMGVRVLRAEEPGTVSTIVREALDAAFGREEAVA
                     VLLSQKLVGRKDWGDGK"
     gene            6819..7358
                     /locus_tag="P5164_10430"
     CDS             6819..7358
                     /locus_tag="P5164_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012329653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRY44352.1"
                     /translation="MAACPDALTVAGLGAPAYDLFAAGDRDTSFYLWGGMGGAAAMGL
                     GLALAQPERSVVVLTGDGEQLMGLGALATIGAQRPPNLTIVVLDNGRYGETGMQETHT
                     SLGTSLAGVAAACGLTWAETVTTTEALRDLARRVRARGGCGLAQVLVRAESPARALPP
                     RDGRLLQGRFRASLGLPAA"
     gene            complement(7386..>8042)
                     /locus_tag="P5164_10435"
     CDS             complement(7386..>8042)
                     /locus_tag="P5164_10435"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HRY44353.1"
                     /translation="AAWQETAEKTGGDYYDVMEAPLGALDLLVCDVSGHGLASTVLMS
                     AARAYLRALHLVEADPRRLIEKLNRLISPDIPDDAFATLVLCRIEPSGAACYVSAGHL
                     PPLVYRARTRAFDRLEETDLVLGWSETTSYRAHAIEPLAPGDLVVLATDGLYEASSPE
                     GLLWGVDAAKEAISAAAREGADGIIEALRAGAFGHAEKLFLEDDITLLVAEKVEARPA
                     "
BASE COUNT          959 a         2827 c         3058 g         1198 t
ORIGIN      
        1 tcgaggaagc gcttcgtgaa cgtcacggcg tcctcgagcc gcgtcggccc ctcgatcggg
       61 cagccccaga tcgtgctgac ggtcccgttc acgcggatgc cggcgtcgtg agccttccgg
      121 atgcagcgct ccgcctcggc ccagtacccg gcgtggtcga ggcccgagtt cttcttcatg
      181 tgcgaggccg aggtcgagac catcatcagg atccggtccg gcccccaccc ttcggccttc
      241 gcggcgatcg cccggtcgac cgccttctcg cggatcgtca cggccgtcag ctcggtctcg
      301 ccgatgagct tcgccacgcg ggcgctgccg cggatcgcct tcagcagctc gtctgcgtcg
      361 cggaactgcg gcatcccctt cgggttcccg aggttcgtca cctcgaggcg ccggtagccg
      421 gcgaggatca gctcctcgag gacccagagc ttcgcctcgg tcgggatcca cctctcctcg
      481 tgctggaagc cgtcgcggac ggtgatgtcg gccaggtcga cgcgcttcgg aaggttccag
      541 gtcttcatgc tcacctcagg agctcgcggg cggcgagcac ggtaaaggcg tgggtctcga
      601 acgtgacggc gacgctcccg aggtggcccc ccttcaggta gtcgaggccg aggggatgga
      661 ccttcgcccg ctcgacgagc tcggcgacga cggcggcgtc gcgcgggtcg acggggtggg
      721 cgtggagcgg agcggaagcg ccgagaaggg cgtgcaggag ctcgtcagga gtcgcgtggg
      781 cgtggtggac cacgtcggcg agggcggcga tcaccttctc gacctcgggc tccggcaggc
      841 cggttcgagc cgcggcgtct ttcacgaggg aatcgtggtt catcgggcgg tctccttcgt
      901 cggcgccgtg ggcgggagga cggggccgct ccggtcggcg agcgccccga ggaaggcgac
      961 gatcgaggcg atctcgtcgt cggtcagctc cttgccgagc tggtgccagg ccatcttctt
     1021 taccgccgtc tcgagccggt cgacggagcc gtcgtggaag tacgggcccg tcgccacggc
     1081 gttccggaga gagggcacct tgaacttccg gcggtcttcc tcgttcccgg tgacggcgaa
     1141 gcgcccctcg tcgtccgtgt cccacggcct gacgaggccg aggacctgga actggttcgc
     1201 gccgatcgtc ggcgagttgt ggcacgtcgt gcagcccgtc gccaggaaga gcttcaggcc
     1261 cgcggcctcg cggttcgaga gcgcgtcgag gtccccgcgg aggaagtcgt cgaggcggtc
     1321 gcgcgtcttc agggtccgct cgaacgcggc cagggcccgg gcggtgttct tcagggtgat
     1381 cggctccgcc tcgccgggga aggcggcggc gaacggctcg ccgtacgagg cggcgccccg
     1441 caaagccgcc tccacggcgg ccgcgtccgg catcgccatc tcgatcgggt tcaggatcgg
     1501 gccctcggcc tgctcctcga gcgtcttcgc ccggccgtcc cagaactgcg cgacgtgata
     1561 gccggcgttc ttcacgctcg gggagttgcg cggccccttc gtccccttcg ccccgtccga
     1621 ggtcgcctcg ccgtcggcgc ccgtcgggtg ctcgccgtcg aggcggtgac agtcgttgca
     1681 ggactgggtt ccggtgaccg agagcttcct ttcgaaccag agggaccggc cgagggcgac
     1741 gagctggggc gaatcggaat ccgcccccgg catcttctcg ggaaggacgc cgagcgtcgt
     1801 gctcgcctgc gccgcgagcg cctttcgctc ttcgtgtccg atgcgcggct ccggcgcgct
     1861 ccgcgagcag gcggcaaggg tcaggacggc ggccgctgcg gcgagacgag atgcccgctt
     1921 catggaggcg ggattctaga cggacggcgc gccgcccttc ggaagcgctt cccaacgccc
     1981 ctcgcggctc ctacgatgtc ccttcgatga gaagcccctg gtttcggcgg ggcggcgtcc
     2041 tcctcgggat cgtcgccgcc gtgctcctcc tccgtgcgac gctcttccgt ccggaccccg
     2101 tccccgtgac cgtcgtagcc gccgcgaagg ggacggtgga ggagacggtg gcgggcagcc
     2161 gcgccgggac gatgaggtcg cgccgccgcg cgacgctcag ccccgaagtg gggggccgcg
     2221 tcgagcggct cgccgtccgc gagggggacc gggtgaagaa gggcgacctc ctcgtccgca
     2281 tcgcggcgga cgacgtgaaa gcccaggtcg ccctcgcgga gaagtcgctc gccgtcgcgc
     2341 aggcggccga ggtcgaggcg tgccgcgccg cggagcaggc gcgccgggac ctcgcgcgga
     2401 gccggagcct cgcgaaggac gaggtcctct ccgtcggcct cctcgagaag gcggaaacgg
     2461 aggccgggat gacgggggcg gcgtgcgagg cggcgcgcgc ccgcgtcggg caggcgcgcg
     2521 ccgccctcga cgtggcgcgg atcgccctcg cgaagaccgt cctgaaggcg ccgttcgacg
     2581 gcgtcgtcgc agaggtctcg acggaggagg gggagtgggt gaccccctcg ccgcccgggc
     2641 tcccgatccc gcccgtcgtc gagctcttcg acccggacga cgtctacgtg agcgccccgc
     2701 tcgacgaggt ggacgtcggg agggtgaaga agggggccga ggtccgcgtc acgttcgacg
     2761 cgtaccccgg gcggacgttc gccggccggg tgacgcgggt cgccgactac gtcctcgacc
     2821 agagggagca gaaccggacc ttcgaggtgg aggtcgcgct cgacgattcc tccttcgcgc
     2881 ggacgctcct tcccgggacg tcggccgacg ccgtcgtcgt cctcgcgcgg aaggaggagg
     2941 tcctccgggt gcccgcctac gcgatcctgc cgggaggccg gatcctcgtc ctctcgaagg
     3001 ggcggctcgc cggggcgaag gtggagacgg ggctgaaggg ggcggagtgg gccgaggtcg
     3061 tctccggcct ttccgccggc gacctcgtcg tcacgtcgct cgaccggccg gaggtgaagg
     3121 agggggcgag ggcgcgcgcc gaggaggcgc ggtgatcgag ctctccggcg tgtcgcggac
     3181 gtgggaggtg ggggggcgcc ccgtccacgc ccttcgggac gtgacgctct cgatcggctc
     3241 cggggagtac gtctccgtca tgggaccctc gggctcgggc aagtcgacgc tcctctccct
     3301 cctcggaggg ctcgaccggc cgacggccgg ctcgtaccgt ttcgaggggc gcgaggtcgc
     3361 gagccttccg gacgacgatc tctcgcgcct ccgccgcgag cggatcggct tcgtcttcca
     3421 gtcgttccac ctcgtcccgc ggatgacggc gctcgagaac gtggcgctcc cgctcctcct
     3481 cgcgggcgtc tcaccgaagg accggacggc tcgcgccgcc gaggcgctcg cctcggtcgg
     3541 cctctcgccc cggctcgacc accgccccga ccagctctcg ggcggagaga agcagcgggt
     3601 ctgcctcgcg cgggccgtcg tgacgcggcc gggcctcctc ctcgccgacg agccgaccgg
     3661 caatctcgac agcgcctccg ggaccgagat cgtcgcgctc ctcgagcggc tccacgcctc
     3721 ggggatcacg ctcctcgtcg tgacgcacga cgccgggatc ggcgggcggg cgcggcgacg
     3781 ggtgcgcctc gccgacgggc gcgtcacggg ggacgaggcc gcgtgagagg gggcgacctc
     3841 ctcgccttcg ccgccggggc gttccggggt cacaagctcc gcacctcgct ctcgctcctc
     3901 ggcgtcgcga tcggcgtggc ggccgtgatc ctcctgacga gcctcggcga gggggcgagg
     3961 cgctacgtga cgggggagtt cgcactcctc ggctcgaacc tcgtcatcgt cctgccgggg
     4021 aagtcggaga cgaccggcgt cgtgccggtg ggcggcgtgc cgcacgacct gacgctcgag
     4081 gacgtcgagg cgctccggcg gcgcgtctcg ctcctcgtct ccgtggcgcc gctctccgtc
     4141 ggagggctca cggctcgctt cggggagcgg agccgggacc tgaccatcgc cggcgtcacg
     4201 tcggagtgga aggacgtgag gcgcctcgcg ctccgagagg gggccttcat cccgcccggc
     4261 gacccggacc gggcgccccg cgtctgcgtc gtcggggcaa aggtcgcgtc ggagctcttc
     4321 ccgggccgct cgccggtcgg ggagctcctc cgcctcggcg aggagcggtt ccgggtgacg
     4381 ggggtcctcg tcccgcgcgg ggtctccgtc ggcctcgacc tcgacgaggt cgtcctcgtc
     4441 ccgatcggcc accacctccg gatgttcgac cggcgctccg tcttccgcgt cctctgcgag
     4501 gcgcggacct cggaagacct cgaggcggcg aaagccggga tcctcgccgt cctgaaggac
     4561 cggcacgacg gcgaggagga cgtcaccgtc ctgacgcagg acgccgtcat gaagacgttc
     4621 gggaaggtcc tcggcatcct cacggcggcg ctcgccggca tcgcggcggt ctccctcacc
     4681 gtcgccgggg tcgggatcat gaacgtcatg ctcgtctccg tctccgagag gacgcgggag
     4741 gtcggcctcc tgaaggcgct gggcgcgagc ggcgggcagg tcctccggct cttcctcgtc
     4801 gaggcggcgc tcctctcgag cgcgggcggg gcgctcggcc tcctcgccgg gtgggccggg
     4861 acgctcgcga tccgggccct ctacccagac ttcccggtcg ccccgcccgc ctgggccgtc
     4921 gccgcggcgc tcgccgtctc ggtctcggtc ggcctcgtgt tcggcgtcct gccggcgcgg
     4981 cgggcggccc ggctcgatcc ggtcgtcgcg ctcgcgcgaa ggtgaaggcg tggcgttcgc
     5041 cgacgtcctc cgcctcgccc tcggctccgt gacggcccac cgcctccggt cggcgctgac
     5101 ggtcctcggg atcgtcatcg gcatcgcctc ggtcgtcctc ctcacctcgc tcggcgaggg
     5161 gactcggcag gcgatcctct cggagttctc gcagttcggg acgaacctcc tcgcggtgac
     5221 gtcgggaaag atcgagacgc acggcatcgc cggggccgtc ggcgggacga cgcggcctct
     5281 gacgctcgac gacgccgaag cgctccgcgc cgtcccgggg atcgagaagg tcgtccccgt
     5341 cgtcttcggg acggcccgcg tctcctccgg ggagcgggcg cgggacacgt tcgtctacgg
     5401 cgcgacctcg gacggcccgg aggtctggcg gttccaggtg cgcctcggcc gcttcctccc
     5461 cgagacggag ctcgaccggg cgacgccggt cgccgtcctc gggccgaagc tgaagcggga
     5521 gctcttcggg ggtgagaacg cgatcggtcg gcggattcgc gtcggcggtc accgcttcac
     5581 cgtcatcggc gtcatggcga ccgtcgggca gttcgtcggc ttcacgctcg acgacgcggt
     5641 ctacgtcccc gtcgcctccg cccagcagct cttcgaccag gcgcaggtga tggagatcga
     5701 cgcgaccttc acccccggcc tcccgccgga gacggtcgtc gcggcggtga agcgcgtcct
     5761 gaaggcccgg cacggcggcg aggaggactt caccgtcctg acgcaggggg agatgctcga
     5821 gtcgttcgga cgcgtctttg cggtcgtcag cgtcgccgtc ggagccatcg gggccatctc
     5881 cctcctcgtc ggcgcgatcg ggatcctgac gatgatgtgg atcgcggtcg gggagcggac
     5941 ggcggagatc ggcctcctga aggcgctcgg ggcgacgcgc ggcgacgtga ggaacgtctt
     6001 cctcgcggag gcggcgatcc tctccggcgc gggaggcctc gccgggatcg ccgcggcgat
     6061 gtcgctcggc gcgatcgtga ggctcgccct cccggcggtg ccgttcgcga cgccgccgga
     6121 gtacgccgcg gcggcgctcg cgacggcggt cggcgtcgga ctcgtcgcgg gcgtggcgcc
     6181 ggcgcggcgc gcggccgcgc tcgacccggt ggagaatctg cgggcggagt gagcggcgtg
     6241 agcgatgcgg tcccggcgtc ctggcaggag gaggtcttct cggccctcag ggaggccggc
     6301 gttcgccagg tggcctgggt cccggacgcg gggcacgcgc acgtcatcga tcgcgtcctc
     6361 gtcgacccgg agatgctcgg catcccgctc acgacggagg aggaaggcgt cgcgctctcc
     6421 tgcggcgcct ggctcggcgg agatcgcgcc gtcctcctga tgcagtcgag cggcgtcggg
     6481 aactgcgtga acatgctctc cgtcgtctcg acgtgccggt tcccgttcct ctccctcgtc
     6541 acgatgcgcg gcgagtggga ggagcggaac gagtggcagg agccgatggg gagggcgacg
     6601 ggaacggtgc tcgaggcgat gggcgtgcgg gtcctgcgag ccgaggagcc gggcaccgtg
     6661 tcgacgatcg tccgcgaggc cctcgacgcc gcgttcggac gcgaggaggc ggtcgccgtc
     6721 ctcctctcgc agaagctcgt cggccgaaag gactgggggg acgggaagtg accgcgcctc
     6781 tccggacgct cgatcggcgc gccttcgtct cccggctgat ggcggcctgc cccgacgccc
     6841 tgacggtggc cggcctcggc gcgccggcct acgacctctt cgcggcgggc gaccgcgaca
     6901 cgagcttcta cctctggggc ggaatggggg gcgccgcggc gatggggctc ggcctcgccc
     6961 tcgcgcagcc ggaacggagc gtggtcgtcc tcacgggcga cggcgagcag ctgatgggcc
     7021 tcggagcgct cgctacgatc ggggcgcagc gtccgcccaa cctcacgatc gtcgtcctcg
     7081 acaatggtcg ctacggggag accgggatgc aggagacgca cacgagcctc gggacgagcc
     7141 tcgcgggcgt cgcggcggcc tgcggcctca catgggccga gacggtcacc acgaccgagg
     7201 ctctccggga cctcgcgcgt cgggtccggg cgcgcggggg ctgcggcctc gcgcaggtcc
     7261 tcgtccgggc cgagagtccg gcgcgggcgc ttccgccgcg cgacggccgg ctgctgcagg
     7321 ggcgtttccg agcgtctctc ggcctcccgg cggcctgaag agaagcggcc gcgcggggcg
     7381 cccggtcagg ccggccgggc ttcgaccttc tcggcgacga gcagggtgat gtcgtcctcg
     7441 aggaagagct tctcggcgtg gccgaaggcg ccggcgcgga gggcctcgat gatcccgtcg
     7501 gcgccttctc gggccgccgc ggagatcgct tccttcgcgg cgtccacacc ccagagcagg
     7561 ccttcggggc tcgaggcctc gtagaggccg tcggtcgcca ggacgacgag gtcgcccggc
     7621 gcgaggggtt cgatggcgtg tgcccggtag ctcgtcgtct ccgaccatcc gaggacgagg
     7681 tcggtctcct cgagccggtc gaacgcccgg gtgcgggcgc ggtagacgag cggggggagg
     7741 tgcccggcgg agacgtagca ggccgcaccg gagggctcga tgcggcagag gacgagcgtc
     7801 gcgaacgcgt cgtccgggat gtcgggggag atgagccggt tgagcttctc gatcagcctg
     7861 cggggatcgg cctcgacgag gtggagagcg cggaggtagg cccgtgccgc cgacatcagg
     7921 accgtggagg cgaggccgtg gccggagacg tcgcagacga ggaggtcgag cgcgccgagc
     7981 ggagcctcca tgacgtcgta gtagtcgccc ccggtcttct ccgccgtctc ctgccaggcg
     8041 gc
//
LOCUS       DAOTEZ010000175         7958 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_6434_length_7958_cov_7.220676, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000175 DAOTEZ010000000
VERSION     DAOTEZ010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7958
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6434_length_7958_cov_7.220676"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="P5164_10440"
     CDS             <1..1026
                     /locus_tag="P5164_10440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY44354.1"
                     /translation="RELDEGEWEGEMSTFADLDLGRSFFARPVTVAAATGADVAEILT
                     RMGKQSAADELNCGACGYDTCREHAAAVHSGLAESEMCLPFTIDQLRNAVREIAVSHD
                     QLASAQQALMHSERLASMGQLAAGIAHEVNNPLGVVLLYSHLLLEGIPEGSALRGDLE
                     TVAAEADRCKKIVSGLLDFARQNKVSLSRVTVDDLVAQSVRGVVVPERVVVEVEHEDP
                     GLVVELDRDQIVQVVANLVRNAAEAMPRGGTVIVRTRAVGGSLVLSVTDGGTGISKEN
                     LSRVFEPFFTTKPIGKGTGLGLPVSYGIVKMHRGDIDVVSNADPAAGPTGTVLTVRLP
                     LRGTRGA"
     gene            1030..2190
                     /locus_tag="P5164_10445"
     CDS             1030..2190
                     /locus_tag="P5164_10445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HRY44355.1"
                     /translation="MGGTLKVLVVDDEPGIRLAVSRVLEPHRATLPDETEETAFEVAQ
                     AATGEEALAACTAGAPDILLLDYKLPGISGLDVLERLGAATAETIVVMITAYATIETA
                     VTATRRGAYDFLAKPFTPAELRTTVSRAATHLALQRRARRLAEEKRQARFQLLSVLFH
                     ELKSPLAAVEGYLDVLRAGFLPPGSAQYDEVLERSRVRLDGMRRLVVDLLDLTRLESG
                     QRKREPVALDAKDVVLRAVETVQPAAAARGIELVVDAPEAVPLVADPGELEIVLNNLL
                     SNAVKYNREGGRVDVTLSKDAGGARLAVADTGIGMTAQEVGRLFGEFVRIRNEKTRDI
                     PGSGLGLSIVKKVVELAGGEVSVESEPGKGTTFTVRLPSGAPPPVLAAPHAD"
     gene            2423..2848
                     /locus_tag="P5164_10450"
     CDS             2423..2848
                     /locus_tag="P5164_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY44356.1"
                     /translation="MARILIVDDDKDIVEACRLFLEREGHTVAAAYSRGGGMRAVSDW
                     PPDLLVLDVMMDEPDDGIRMAQELRRSGWTTPIVMLTSIGKVTGLGYRADPDVLPVDA
                     FLEKPVRPAVLLKTVADLLARSVGKEGSHAGHGTAPAAR"
     gene            2814..3335
                     /locus_tag="P5164_10455"
     CDS             2814..3335
                     /locus_tag="P5164_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRY44357.1"
                     /translation="MLVTERLRLHDDIGAWVEKHGATRAALIPVLQEIQRSYNEISEY
                     AMQEVADRLDIHPVEVYSVVSFYSFLESKARGRFVIRLCRTVSCDMTGKDRVARQLEN
                     DLGIRFGETTRDGNFSLEWANCLGMCDQGPALLINDQVFTSVTPETVHDLIQACRKSF
                     GPLAVQGQKGRAH"
     gene            3335..4525
                     /locus_tag="P5164_10460"
     CDS             3335..4525
                     /locus_tag="P5164_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRY44358.1"
                     /translation="MSAVRKGLVTFAASVPEAGLKAALARPRSEVIAEVTASKLRGRG
                     GAGFPTGVKWNLAAAATAPAKWVVCNADEGEPGTFKDRVILSEAPDLVLEGMTIAAWA
                     IGAARGILYLRGEYAYLRDSLEKVLARRREGGLLGTGILGRELAFDVEIRMGSGAYVC
                     GEETALIESLEGQRGEPRNRPPFPVDCGYRSEPTIVNNVETFACAAMVLAKGAGWFRG
                     MGTEVSTGPKLFSVSGDCREPGVYEFPLGITIAELLAAVGGEGAKAVQVGGASGRCVP
                     AAEFGKTLAFEGVPTGGSIIVFGPERDMLHVAENFLEFFVEESCGQCTPCREGNVKLL
                     EGVKLLREGRCSWAYLQELMKLGEVMQLASKCGLGQSSPNAFLSIVEHFRPEIMGRTA
                     GGNA"
     gene            4538..6478
                     /locus_tag="P5164_10465"
     CDS             4538..6478
                     /locus_tag="P5164_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HRY44359.1"
                     /translation="MSSRIERTASISRAPVSQRGHVVPVSADPEAIGGTVSVTIDGIE
                     AKVPLGTTVLDAARSLGLKIPTLCHHPDLEVAGVCRICVVEVEGQRTLQAACAFPITA
                     PIKVQTHTRKVRQARRRILDLMLSKHVGECYTCFRNLNCELQTLAKEYGVDFFRFGHE
                     GTPRHEVDDSSYSVRRNMDKCILCRRCVRTCIDLQEVGVLEAVGRSEKTRIATFLDKP
                     LNDVVCINCGQCINRCPTAALGAMDPSDEVWKDINDPGKHVVIQTAPAPRAAIGECFG
                     LPPGTPLTFEMNTAMKTIGFDRVFDTNFTADLTIIEEGTELLLRLHEALVQKKPVALP
                     QFTSCSPGWVKYLEHFYPEMIPNLSTAKSPQQMFGALIKTFYAEEAGIDPASIVTCAL
                     MPCSAKKYECNRPEMCDSGHKDVDYGLVTRELGQMFHEAGIDLPKMPKGGFDDPFGTA
                     TGSGVIFGATGGVMEAALRTVVELVTGLKIEDVFAHADVLPVRGFDGVRYVELPIPAI
                     GEVPELLKMNKALPFSDWKWLEGAVLKVAVCHGTANAKRVVEDIKAGGVFSKCHFIEF
                     MACPGGCLGGGGMPIPTSPEIRAARAKAIYAEDSSYEIRKAHENPAVIRLYREFLTDG
                     PCGEKSHHLLHTHYTPRGKYIA"
     gene            6549..7796
                     /locus_tag="P5164_10470"
     CDS             6549..7796
                     /locus_tag="P5164_10470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRY44360.1"
                     /translation="MSRGRAVPPSALAPREAEALDLVNRKVAAGRSLDEVLDFLFRAT
                     RDVCPCDRIGVAFLEEEGQRVVARKAVADYEPLLLAAGWAEDLAATSLGPLLASRTPR
                     VLDDLPAYLAARPASRATRLLVDEGARSSLTARLEVEGRVFGFLFRSARRMRAYDARQ
                     AYLHVAVSERLAQAVEKAWLLDRLAEANAGYVEMLGFVSHELKAPLASLVMDGETLLG
                     GYAGQVDETARSAVDRMTRKAAYLGRLVKEYLDLARVEERDLAVRRVACDFVADVVEP
                     ALDVVLALLEDRRCRLVRDLPLGPLAVSADPELMTVVLVNLLGNAAKFAREGGEVRIC
                     ARRDGDRLRVAVRNEGPGFRPEERSRLFRRFSRLPDPELAGRPGSGVGLYTCRRIVAL
                     HGGRIDAHSEPGRWAEFRFEVPA"
BASE COUNT         1201 a         2819 c         2819 g         1119 t
ORIGIN      
        1 cgggagctcg acgaggggga gtgggagggc gagatgagca cgttcgccga cctcgacctc
       61 gggcgctcct tcttcgcccg cccggtcacg gtcgcggcgg cgaccggagc ggacgtcgcg
      121 gagatcctga cgcggatggg gaagcagtcg gcggccgacg agctgaactg cggggcgtgc
      181 ggctacgaca cctgccgcga gcacgcggcc gcggtccact ccgggctcgc cgagtcggag
      241 atgtgcctcc ccttcacgat cgaccagctc cggaacgcgg tccgcgagat cgccgtctcg
      301 cacgaccagc tcgcctcggc ccagcaggcg ctcatgcatt ccgagcgcct cgcgagcatg
      361 ggccagctcg ccgccggcat cgcccacgag gtcaacaacc ccctcggcgt cgtcctcctc
      421 tactcccatc tcctcctcga ggggatcccg gagggctccg cgctccgggg agacctcgag
      481 acggtcgccg cggaggcgga ccgctgcaag aagatcgtct cggggctcct cgatttcgcc
      541 cggcagaaca aggtctcgct ttcgcgagtg accgtcgacg acctcgtggc tcagagcgtc
      601 cgaggggtcg tcgtgcccga gcgggtcgtc gtcgaggtcg agcacgagga ccccgggctc
      661 gtcgtcgagc tcgaccgcga ccagatcgtc caggtcgtcg cgaacctcgt ccgcaacgcg
      721 gccgaggcga tgccgcgggg cgggacggtc atcgtgagga cgcgggcggt gggcggctcg
      781 ctcgtcctct cggtcacgga cggcggcacg gggatctcga aggagaacct ctcccgggtc
      841 ttcgagccgt tcttcacgac gaagccgatc gggaagggca cggggctcgg gctgccggtg
      901 agctacggga tcgtgaagat gcatcgcgga gacatcgacg tcgtctcgaa cgccgacccc
      961 gcagccggac cgacggggac ggtcctgacc gtgcggctcc cgctgcgcgg gacgcggggc
     1021 gcctgagaga tgggcggcac gctgaaggtc ctcgtcgtcg acgacgagcc gggaatccgg
     1081 ctggcggtgt cgagggtcct cgagccgcac cgggcgacgc tccccgacga gacggaggag
     1141 acggcgttcg aggtcgcgca ggcggcgacc ggcgaggagg cgctcgcggc ctgcacggcg
     1201 ggcgcgcccg acatcctcct cctcgactac aagctcccgg gcatctccgg gctcgacgtc
     1261 ctcgagcggc tcggagcggc gacggcggag acgatcgtcg tcatgatcac ggcctacgcg
     1321 acgatcgaga cggcggtgac ggcgacgcgc cgcggcgcgt acgacttcct cgcgaagccg
     1381 ttcacgccgg cggagctccg gacgacggtg agccgcgccg cgacccacct cgccctccag
     1441 cggagggcga ggcgcctcgc cgaagagaag cggcaggcgc ggttccagct cctctccgtc
     1501 ctcttccacg agctgaagtc gccgctcgcg gccgtcgagg gctacctcga cgtcctccgg
     1561 gcggggttcc tcccgccggg ctccgcgcag tacgacgagg tcctcgagcg gagccgcgtg
     1621 cgtctcgacg ggatgcgccg cctcgtcgtc gatctcctcg acctgacccg cctcgaatcg
     1681 gggcagagga agcgcgagcc ggtcgcgctc gacgcgaagg acgtcgtcct ccgggccgtg
     1741 gagacggttc agccggccgc cgcggcgcgg gggatcgagc tcgtcgtcga cgcgccggag
     1801 gccgtgcccc tcgtggccga cccgggagag ctcgagatcg tcctgaacaa cctcctctcg
     1861 aacgccgtga agtacaaccg cgagggcggc cgggtggacg tgacgctctc gaaggacgcc
     1921 ggcggcgctc gcctcgccgt cgccgacacc gggatcggga tgacggcgca ggaggtcgga
     1981 agactgttcg gggaattcgt ccgaatccgg aacgagaaga cgcgcgacat ccccgggagc
     2041 ggcctcggcc tgtcgatcgt gaagaaggtc gtcgagctgg ccggcggcga ggtgagcgtc
     2101 gagagcgagc ccgggaaggg gacgacgttc accgtccggc tcccgtccgg agcccctcct
     2161 ccggtgctcg cggccccaca cgccgactga ggacgcaccg ccggagcacc ctgtcgggtt
     2221 ccggaacgac cgcgaataca gacccggaag cggcctgttt ccaatgactt ccgcctcccg
     2281 gcctgcctcc gctggtgata ggctgctcac tccatgaatc ggggacccgg ccgctacggt
     2341 ccggcatggg cggcgacgga gccgtccccg gtgcacctct cttcgacagg tcgaatcccg
     2401 ggattcggac aaggaggtca cgatggcgag gatcctgatc gtcgatgacg acaaggacat
     2461 cgtcgaagcc tgccgcctct tcctcgaaag agaggggcac accgtagcgg ccgcctacag
     2521 ccggggcggc gggatgaggg ccgtctcgga ctggcctccc gacctcctcg tcctcgacgt
     2581 catgatggac gagccggacg acgggatccg gatggcccag gagctccgcc gctccggctg
     2641 gacgacgccg atcgtgatgc tcacgagcat cggcaaggtc accggtctcg gctaccgcgc
     2701 cgacccggac gtcctccccg tcgacgcgtt cctcgagaag cccgtccggc ccgccgtcct
     2761 cctgaagacg gtggccgatc tgctcgcccg ctcggtcggc aaggagggaa gccatgctgg
     2821 tcacggaacg gctccggctg cacgatgaca tcggggcgtg ggtcgagaag cacggcgcca
     2881 cgcgcgccgc gctgatcccc gtcctccagg agatccagcg gagctacaac gagatctccg
     2941 agtacgcgat gcaggaggtc gccgaccgcc tggacatcca tcccgtcgag gtctacagcg
     3001 tcgtcagctt ctacagcttc ctcgagtcga aggcccgcgg ccgcttcgtc atccgcctct
     3061 gccggacggt ctcgtgcgac atgaccggga aggaccgcgt cgcgcggcag ctcgagaacg
     3121 acctcggcat ccgcttcggc gagacgaccc gcgacgggaa cttctcgctc gagtgggcga
     3181 actgcctcgg gatgtgcgac caggggccgg cgctcctcat caacgaccag gtcttcacgt
     3241 cggtgacgcc ggagacggtc cacgacctga tccaggcgtg ccgaaagagc ttcggcccgc
     3301 tcgccgtcca ggggcagaaa gggagggcgc actgatgagc gccgttcgta agggcctcgt
     3361 cacgttcgcc gcctccgtgc ccgaggcagg cctgaaggcc gctctcgcgc gcccgcggtc
     3421 cgaggtcatc gcggaagtca ccgcctcgaa gctccgcggg cgggggggcg ccggcttccc
     3481 gacgggggtg aagtggaacc tcgccgcggc ggcgaccgcc cccgcgaaat gggtcgtctg
     3541 caacgcggac gagggagagc ccgggacgtt caaggaccgg gtgatcctct ccgaggcgcc
     3601 ggacctcgtc ctcgagggga tgacgatcgc ggcctgggcg atcggcgccg cgcgcgggat
     3661 cctctacctc cgcggcgagt acgcctacct gcgcgactct ctcgagaagg tcctcgcgcg
     3721 gaggagggag ggagggctcc tcgggacggg gatcctcgga agagagctcg ccttcgacgt
     3781 cgagatccgg atgggctcgg gagcctacgt ctgcggcgaa gagacggcgc tcatcgagtc
     3841 gctcgagggg cagcgcggcg agccgcggaa caggcccccg ttcccggtcg actgcggcta
     3901 ccggtcggag ccgacgatcg tgaacaacgt ggagacgttc gcctgcgccg cgatggtcct
     3961 cgcaaagggc gccgggtggt tccgggggat gggaacggag gtctcgaccg ggccgaagct
     4021 cttcagcgtc tcgggcgact gccgcgagcc cggcgtgtac gagttcccgc tcgggatcac
     4081 gatcgccgag cttctcgccg ccgtgggcgg ggaaggcgcc aaggccgtcc aggtcggcgg
     4141 cgcctcgggc cggtgcgtgc cggccgcgga gttcggaaag acgctcgcct tcgagggggt
     4201 cccgacggga ggctcgatca tcgtcttcgg gccggagcgc gacatgctcc acgtcgccga
     4261 gaacttcctc gagttcttcg tcgaggagtc gtgcggccag tgcaccccct gccgcgaggg
     4321 gaacgtgaag ctcctcgagg gcgtgaagct gctgcgcgag gggcgctgct cgtgggcgta
     4381 cctccaggag ctgatgaagc tcggagaggt gatgcagctc gcttccaagt gcggcctcgg
     4441 gcagtcgagc ccgaacgcct tcctctccat cgtcgagcat ttccggccgg agatcatggg
     4501 ccggacggcc ggcgggaacg cctgaggagc agagccgatg tcgagccgca tcgaacgcac
     4561 cgcctccatc tcccgcgccc ccgtcagcca gcgcggccac gtcgtcccgg tctccgccga
     4621 ccccgaagcg atcggcggga ccgtctccgt cacgatcgac ggaatcgagg cgaaggtccc
     4681 gctcgggacg accgtcctcg acgccgcccg ctccctcggt ctgaagatcc cgaccctctg
     4741 ccaccacccc gacctggaag tggccggcgt ctgccggatc tgcgtcgtcg aggtcgaggg
     4801 gcagcggacg ctccaggcgg cctgcgcctt cccgatcacg gcgccgatca aggtgcagac
     4861 ccacacgcgc aaggtccggc aggcccgccg gcgaatcctc gacctgatgc tcagcaagca
     4921 cgtcggggag tgctacacct gcttccggaa cctgaactgc gagctccaga ccctcgcgaa
     4981 ggagtacggc gtcgacttct tccggttcgg ccacgagggg acgccgcggc acgaggtcga
     5041 cgactcctcg tactccgtcc ggcggaacat ggacaagtgc atcctctgcc gccgctgcgt
     5101 gaggacctgc atcgacctcc aggaggtcgg cgtcctcgag gccgtggggc gcagcgagaa
     5161 gacgcgcatc gcgacgttcc tcgacaagcc gctgaacgac gtcgtctgca tcaactgcgg
     5221 ccagtgcatc aaccgctgcc cgacggcggc cctcggcgcg atggaccctt cggacgaggt
     5281 ctggaaggac atcaacgacc cgggcaagca cgtcgtcatc cagacggccc cggcgccgcg
     5341 cgcggcgatc ggggagtgtt tcggcctccc gccggggacg ccgctcacgt tcgagatgaa
     5401 cacggcgatg aagacgatcg ggttcgaccg cgtcttcgac acgaacttca ccgccgacct
     5461 gacgatcatc gaggagggga cggagctcct cctccggctc cacgaggcgc tcgtccagaa
     5521 gaagccggtc gcgctcccgc agttcacgag ctgctcgccc ggctgggtga agtacctcga
     5581 gcacttctac ccggagatga tcccgaacct ctcgacggcc aagagccctc agcagatgtt
     5641 cggggcgctc atcaagacgt tctacgcgga ggaggccggc atcgacccgg cctccatcgt
     5701 cacgtgcgcg ctcatgccgt gctcggcgaa gaagtacgag tgcaaccgcc ccgagatgtg
     5761 cgactccggc cacaaggacg tcgactacgg cctcgtcacc cgcgagctcg ggcagatgtt
     5821 ccacgaggcg gggatcgacc tgccgaagat gccgaagggg ggcttcgacg acccgttcgg
     5881 caccgcgacg ggctcgggcg tcatcttcgg ggcgacggga ggcgtcatgg aggccgcgct
     5941 ccggacggtc gtcgagctcg tcaccggcct gaagatcgag gacgtcttcg cccacgcgga
     6001 cgtcctcccg gtccgcggct tcgacggagt ccgctacgtc gagctcccga tcccggcgat
     6061 cggcgaggtg cccgagctcc tgaagatgaa caaggccctc ccgttctcgg actggaagtg
     6121 gctcgagggg gcggtcctga aggtcgcggt ctgccacggc acggcgaacg ccaagcgggt
     6181 cgtcgaggac atcaaggcgg gcggggtctt ctcgaagtgc cacttcatcg agttcatggc
     6241 ctgcccgggc gggtgcctcg gcggcggggg catgccgatc ccgacctcgc cggagatccg
     6301 ggcggcgcgc gcgaaggcga tctacgcgga ggactcgagc tacgagatcc gcaaggccca
     6361 cgagaacccc gcggtgatcc gcctctaccg ggagttcctg acggacggcc cctgcggcga
     6421 gaagagccac cacctcctcc acacgcacta caccccccgc gggaagtaca tcgcctgacc
     6481 cgcgggcgaa cgaccccgtc cccggtccgg cggagcccgc tcgtcgggct ccgccggagg
     6541 accctcgcgt gagccggggc agagccgtcc cgccctcggc cctcgcgcct cgagaggcgg
     6601 aggccctcga cctggtcaac cggaaggtcg ccgccggacg gagcctcgac gaggtcctcg
     6661 acttcctctt ccgcgccacc cgcgacgtct gcccgtgcga ccggatcggc gtcgccttcc
     6721 tcgaggagga gggacagcgc gtcgtcgcgc gaaaggccgt cgccgactac gaaccgctcc
     6781 tcctcgcggc cgggtgggcg gaggacctcg ccgcgacctc gctcggtccg ctgctcgcgt
     6841 cgcggacgcc gcgcgtgctc gacgacctcc cggcgtacct tgcggcgcgg cccgcgagcc
     6901 gcgcgacgcg cctcctcgtc gacgagggag ctcgctcgag cctgacggcc cggctcgagg
     6961 tcgaggggcg cgtcttcggc ttcctcttcc gaagcgcgcg gaggatgcgc gcctacgacg
     7021 cccgccaggc gtacctccac gtcgcggtca gcgaacggct cgcgcaggcc gtcgagaagg
     7081 cgtggctgct cgaccgtctc gccgaggcga acgcgggcta cgtcgagatg ctcggcttcg
     7141 tgagccacga gctgaaggcg ccgctcgcct cgctcgtgat ggacggggag acgctcctcg
     7201 gcggctacgc cgggcaggtc gacgaaaccg cgcgttccgc ggtggaccgg atgacgagga
     7261 aggcggcgta cctcgggcgg ctcgtgaagg agtacctcga cctggcgcgc gtcgaggagc
     7321 gcgatctcgc cgtccggcgc gtcgcgtgcg acttcgtcgc cgacgtcgtc gagccggctc
     7381 tcgacgtcgt cctcgcgctc ctcgaggatc ggcggtgccg cctcgtccgg gacctcccgc
     7441 tcgggccgct cgcggtctcc gccgaccccg agctgatgac cgtcgtcctg gtgaacctcc
     7501 tcgggaacgc ggcgaagttc gcgcgcgaag gcggcgaggt ccgcatctgc gcgcggcgcg
     7561 acggggaccg gctccgcgtt gccgtgcgga acgaggggcc gggcttccgg ccggaggagc
     7621 gctcgcgcct cttccgccga ttctcgcgcc tcccggaccc ggagctcgcc ggccgccccg
     7681 gctcgggcgt cggcctctac acctgcaggc ggatcgtcgc gctccacggc ggacggatcg
     7741 acgcccactc cgagcccggg cgctgggccg agttccgctt cgaggtcccg gcgtagcacg
     7801 cggggtgcgc gcatccgccg tggggagatc tcggaacccg taagatcgcg gccatgaact
     7861 cggagcatgc gcggcggtcc cgtcccggac tctgtcgacg cgccggcgga gctccgtcga
     7921 cgggaagacg gatcgcggtc ctggccggcg ccctcctc
//
LOCUS       DAOTEZ010000176         7958 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_6435_length_7958_cov_4.293939, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000176 DAOTEZ010000000
VERSION     DAOTEZ010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7958
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6435_length_7958_cov_4.293939"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /locus_tag="P5164_10475"
     CDS             complement(<1..1419)
                     /locus_tag="P5164_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44361.1"
                     /translation="MKRALASILLLAALLAAPALHAQTGNIDPAERHAWAENAGWMDF
                     RPSFGGATVLPTHLTGWAWQESAGWVKLGSSAGGPYANTTAANWGVNLNTVTGALSGF
                     AWSENAGWIRMDPSYGGVTYDGLTKKLSGWAWSESLGWLHFRSTTPTAYGVAVTCGTL
                     TGTVSGGGTVCAGSSSTVTVTVSGGAAPYTVTLNNGGGTQSGAGPVFTFTVSPSSTTT
                     YSVASLTDGLSCAGSGSGSATVTVNSIPATPTAGSNSPVCTGGTITLTTPTVAGATYA
                     WTGPDGFTSALQNPTIPNATTAKAGSYSVTATVNGCPSPAGTTAVVVNATPATPTAGS
                     NTPVCTGSTISLTASTIAGATYAWTGPNGFTSTAQNPTIPSATAAMAGTYSVTATVNG
                     CTSPAGTTSVVVNATPATPTAASNSPVCTGSTITLTTPTVAGATYAWTGPNGFTSSAQ
                     NPTIPNATAAMAGTYSVTVTGGG"
     gene            complement(1416..2699)
                     /locus_tag="P5164_10480"
     CDS             complement(1416..2699)
                     /locus_tag="P5164_10480"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
                     /protein_id="HRY44362.1"
                     /translation="MNAPPPPALPPAPLPAGPAPAARPFAGHPLFSLANVYRAFRACR
                     RRKRGTAGAIAFEARLEDELVALHEELESGTWKPRPFTVFLVSKPKKREIFAADFRDR
                     VVHHVLAGHLTPRWERRFIHDSYACRVGKGTHAGVERLRSFTRQATANGTREAWYLQL
                     DVRGFFCSIDRQRLFDRLARIERDPAVLWLLRTIVFDDPTEGCRLRRVTREELLRLPA
                     HKTLFRAAPGCGLPIGNLTSQLFANVFLDALDQHVKHGLGARWYVRYCDDLVLLSSER
                     AELVALEASIGAFLEERLSLALNERRRLRPVSDGVDFLGYVVRPDYLLVRRRVASALK
                     DRLSTVGPVLADAPAERTRVQRWLASYHAHLAFASSRRLVASLRRRHRWLDETFAFRP
                     GRSPRLRPRARRAAPVARPGRSSNRNTTSRRGVAR"
     gene            complement(2999..3397)
                     /locus_tag="P5164_10485"
     CDS             complement(2999..3397)
                     /locus_tag="P5164_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRY44363.1"
                     /translation="MARYEHLPIYKAAFDLACEAERAVRAFSRYNKYTLGTDLRQKAR
                     GLVDTVVEANGSADRRSVLERLRGQIESFKVLVRLCQEVNAFSSTGVYLSLAERVVGL
                     GKQNEGWLRHASGAATKKSGEAEHGQNLAG"
     gene            complement(3384..3719)
                     /locus_tag="P5164_10490"
     CDS             complement(3384..3719)
                     /locus_tag="P5164_10490"
                     /inference="COORDINATES: protein motif:HMM:NF013765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44364.1"
                     /translation="MVVSQKLIARYAELKAEAPGCLLLMQVGTFLQVQGDDARTVSGV
                     TGLKLQMGGDVDSPVVLGGFPKSGLDSYVGRLVRAGHSVALAFQDEAKERRISEVVRV
                     SVEARDGAV"
     gene            complement(3851..4798)
                     /locus_tag="P5164_10495"
     CDS             complement(3851..4798)
                     /locus_tag="P5164_10495"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HRY44365.1"
                     /translation="MVIVAFVLLAPGMIEAQLAPPAGPNDAASARFTLGDVYQRLLSG
                     AAGVKRGATFTEPAAGPTAAASKTTDEIMAVAPAADNVNGATAADVAAGKTFWGLRTS
                     GGTWGLTTGSGTIATYPAPVAKTGQTQCYKFDGTNWVIDTDCATNTPANQDGKLRKGV
                     AWPNPRFTKNGNGTVTDNLTGLIWLEDGNCTVFYTGDATGQNSRNWTNAMTAANSLAS
                     GACGLTDGSTAGQWRLPNVKELLSLIDYAYEGPALSNTAGTGKWKTGDPFSWVKSDFY
                     WSSTASTNNSAYYVHFWGGWTEGYGRTLSYAVWPVRDGQ"
     gene            5198..5767
                     /locus_tag="P5164_10500"
     CDS             5198..5767
                     /locus_tag="P5164_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010638680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRY44366.1"
                     /translation="MSSLPKRREATYEDLLALPDDVLGQLVEGELHATPRPAIGHQLS
                     VSMLGGELVPPFHSGRGGPGGWWIVDEPELHLGRNVLVPDLAGWRRERLPVLPREAFF
                     TLAPDWVCEVLSPATARIDRLKKLAIYAREGVEHAWLVDPALETLEVFRRQGEAWLLV
                     LTAGGDEVVRAEPFDAIELSLTGLWIRPA"
     gene            5973..7220
                     /locus_tag="P5164_10505"
     CDS             5973..7220
                     /locus_tag="P5164_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY44367.1"
                     /translation="MLEVDLYPRCAEARLVEALTDSPVVLLHGPRQSGKTTLARVVGD
                     RLGYAYVSFDDDVARGGAESDPAGFVADLPDRTILDEVQRVPALFTALKTAVDRRRVP
                     GRFILTGSANVLLVPRISDSLAGRMEILRLHPLAQSELERRSSAFLEALFGRGFEVRQ
                     TERLGRQLAERIAAGGYPAALARPSSRRRTAWYRDYLDALVQRDVRDLARIGSFDALP
                     RLLALAASQTARLLNVTDLASPFQLSRPTIRDYVTLLERVFLVDTLPPWHSNRLSRLV
                     KTPKLHVGDTGLACALLGTDGPALAADRPLLGQLLETFVFQELRRQASCRDEPLTFSH
                     YRDKDGAEVDVVIERSAWELAGVEVKAAATVKDADFRGLRRLRAAAGDRFAGGVVLYD
                     GEISAGFGDRLHAVPLRALWETT"
     gene            7629..7841
                     /locus_tag="P5164_10510"
     CDS             7629..7841
                     /locus_tag="P5164_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HRY44368.1"
                     /translation="MRTNIVLDDELVREASRLTGIRTKRSLVDEALRLLVRTKKRRSI
                     LALKGKIRLAEGYDHKALRERGLGPR"
BASE COUNT         1118 a         2795 c         2826 g         1219 t
ORIGIN      
        1 gccgccgccc gtcaccgtca cgctataggt gcccgccatc gccgccgtcg cgttcgggat
       61 cgtcgggttc tgggccgacg aggtgaagcc gttcgggccg gtccaggcgt acgtcgcccc
      121 cgcgaccgta ggggtcgtca gcgtgatcgt gctgcctgtg cagaccggtg agttcgaggc
      181 ggccgtcggc gtcgcgggcg tcgcgttcac gacgacggac gtcgtcccgg cgggcgaggt
      241 gcagccgttg acggtggcgg tcacgctgta ggtgcccgcc atggccgccg tcgcgctcgg
      301 gatcgtcggg ttctgggcgg tggaggtgaa gccgttgggg ccggtccacg cgtaggtcgc
      361 gccggcgatc gtcgatgccg tcagcgagat cgtgctgccc gtgcagaccg gcgtgttcga
      421 acccgccgtc ggcgtcgcgg gggtcgcgtt cacgacgacg gccgtggttc cggccggcga
      481 cggacagccg ttcaccgtgg cggtgacgct gtagctgccg gccttcgccg tcgtcgcgtt
      541 cgggatcgtc gggttctgga gggcggaggt gaacccatcc gggcccgtcc aggcgtacgt
      601 cgcgcccgcg accgtcgggg tcgtcagcgt gatcgtgccg cccgtgcaga cgggcgagtt
      661 cgagccggcc gtgggagtcg cgggaatgga gttcaccgtc accgtcgcgc tgccgcttcc
      721 ggagccggcg caggagaggc cgtccgtgag ggaggcgacg gagtaggtcg tcgtggagga
      781 gggggagacg gtgaaggtga agaccggccc cgcgccgctc tgcgtgccgc cgccgttgtt
      841 cagggtcacc gtgtagggag ccgctcctcc ggagacggtg acggtgacgg tggaggagct
      901 gcccgcgcag accgtgccgc cgccggagac cgtccccgtg agcgtcccgc aggtcacggc
      961 gacgccgtag gccgtcgggg tcgtgctccg gaagtggagc cagccgaggc tctccgacca
     1021 ggcccagccg ctcagcttct tcgtgaggcc gtcgtacgtc acgccgccgt aggacgggtc
     1081 catccggatc cacccggcgt tctccgacca ggcgaagccc gagagggcgc cggtgacggt
     1141 gttcaggttg acaccccagt tcgccgcggt cgtgttcgcg tacggcccgc cggcgctgga
     1201 gcccagcttc acccacccgg ccgactcctg ccaggcccag cccgtcaggt gcgtcggaag
     1261 gacggtggcg ccgccgaagc tcggccggaa gtccatccag ccggcgttct cggcccaggc
     1321 gtggcgctcg gcggggtcga tgttccccgt ctgggcgtgg agggccgggg ccgcgaggag
     1381 ggcggcgagc aggaggatcg aagcgagggc tcgtttcatc gtgcgactcc tcttcgggag
     1441 gtggtgtttc ggttggacga acgaccgggg cgggctaccg gggcggcccg gcgggcgcgg
     1501 ggccggagcc gcggcgagcg gccgggccgg aaggcgaagg tctcgtcgag ccagcggtgg
     1561 cgccgccgga gggaggcgac gaggcgccgg ctcgaggcga aggcgaggtg ggcgtggtac
     1621 gaggcgagcc agcgctgaac gcgggtgcgc tccgccggcg cgtccgcgag gaccgggccg
     1681 acggtcgaga gccggtcttt gagggcgcta gcaacccggc gcctcacgag gaggtagtcg
     1741 ggcctcacga cgtagccgag gaagtcgacg ccgtcggaga cgggccgaag gcgacgcctt
     1801 tcgttgagcg cgagggagag gcgctcctcg aggaaggcgc cgatggaggc ctcgagggcg
     1861 acgagctcgg ccctctcgct cgacagcagg acgaggtcgt cgcagtaccg cacgtaccag
     1921 cgcgcgccga ggccgtgctt gacgtgctgg tcgagggcgt cgaggaagac gttcgcgaag
     1981 agctggctcg tcaggttgcc gatcgggagg ccgcaccccg gagccgccct gaagagcgtc
     2041 ttgtgggccg gtaggcgcag cagctcctcg cgggtgaccc gccggagccg gcagccctcg
     2101 gtcgggtcgt cgaagacgat ggtccggagg agccaaagga cggccgggtc gcgctcgatc
     2161 cgcgcgagac ggtcgaagag gcgctgccga tcgatcgagc agaagaagcc gcggacgtcg
     2221 agctgcaggt accaggcctc gcgcgtcccg ttcgccgtcg cctgccgcgt gaacgagcgg
     2281 aggcgctcga cgccggcgtg agtccccttc ccgacgcggc aggcgtacga gtcgtggatg
     2341 aaccgccgtt cccagcgcgg cgtcaggtgc cccgccagga cgtggtggac gacccggtcg
     2401 cggaagtcgg ccgcgaagat ctcccgcttc ttcggcttcg agacgaggaa aacggtgaag
     2461 ggtcgcggct tccaggtgcc gctctccagc tcctcgtgaa gggcgaccag ctcgtcctcg
     2521 aggcgcgcct cgaaggcgat ggcgcccgcg gtgccccgct tgcgccggcg gcaggcgcga
     2581 aacgcccggt agacgtttgc cagggagaag agcgggtgtc ccgcgaaggg ccgcgcggcg
     2641 ggcgccgggc cggccggaag gggggcgggg ggaagagccg ggggaggcgg cgcgttcact
     2701 ctccaccccg cacgggccag acgtagttcg tattggtccg gtcgttgttg ttgacgttgc
     2761 cgttgttcac gttcacgttc cacgcgttcg cggggttgtt cacgttggac gacgacgaga
     2821 cccgcccact tcccccggag cgcccgcgcc gcggggcgac ccctccgggg ccaccggctt
     2881 tcgatcccgg tctcccgggt acgagcgcct gaatgctcat gcccgccgcc ctgcctgcga
     2941 cgccggcgac gacgggcggg acgggcgcac agtgatgcgg cacgcccccg cgggcccttc
     3001 agcccgcgag gttctggcca tgctccgcct cccccgactt cttcgtggcg gctccggacg
     3061 cgtgccggag ccacccttcg ttctgtttcc cgaggccgac gaccctctcc gcgaggctca
     3121 ggtagacccc cgtcgacgag aaggcgttga cctcctggca gaggcggacg aggaccttga
     3181 aggactcgat ctggccccgg agcctctcga gcacgctccg ccggtcggcg ctgccgttcg
     3241 cctccacgac ggtgtcgacg aggccgcgcg ccttctggcg caggtcggtg ccgagcgtgt
     3301 acttgttgta ccggctgaaa gccctcacgg cccgctcggc ctcgcacgcc aggtcgaacg
     3361 ccgccttgta gatcggcagg tgctcatacc gcgccatcgc gagcctccac cgagacccgg
     3421 acgacctcgg agatccgccg ctccttcgcc tcgtcctgga aggcgagggc cacggagtgc
     3481 ccggcccgca cgagcctgcc gacgtaagaa tcgagccccg acttcgggaa gccgccgagg
     3541 acgacgggcg aatcgacgtc gcctcccatc tgcagcttca gccccgtcac gcccgaaacg
     3601 gtccgcgcgt cgtccccctg cacctgcagg aacgtcccca cctgcatgag cagcagacag
     3661 ccgggcgcct ccgccttcag ctcggcgtag cgcgcgatca gcttctgcga gacgaccatc
     3721 ccctcagccg gcctcctagc agcgaccccg agaatcggcc ccgccctcag cgggaagggg
     3781 ggcgtaagcc gccctgcacc ccccaaaggg gccaaaggac cgaaggatcc aaagcaccaa
     3841 aagaaccggc ttactggcca tcccgcactg gccagacggc atacgaaagg gttcggccat
     3901 agccctcagt ccagcccccc cagaagtgga cgtagtatgc cgaattattc gtgctggccg
     3961 tggacgacca atagaaatcg cttttcaccc agctgaacgg gtcgcctgtt ttccacttgc
     4021 ctgtgcccgc ggtgttcgac aaagcaggcc cctcgtaggc gtagtcgatc agacttagca
     4081 actctttgac gtttggcaaa cgccattgcc ctgccgtcga gccgtcagta agcccgcagg
     4141 cgccggaggc gagagagttc gccgcggtca tcgcgttggt ccagttccgg ctgttctgac
     4201 ccgtcgcgtc gcccgtgtag aacaccgtgc agttcccgtc ctccagccag atcagccccg
     4261 tcaggttgtc cgtcaccgtc ccgttcccgt tcttcgtgaa cctcgggttc ggccacgcca
     4321 cgcccttccg gagcttgccg tcctggttgg ccggagtgtt cgtcgcgcaa tcggtgtcga
     4381 tcacccagtt cgtcccgtcg aacttgtagc actgcgtctg gcccgtcttc gctaccggcg
     4441 ccgggtacgt ggcaatcgtc ccgcttcccg tcgtcaggcc ccacgtcccc ccgctcgtcc
     4501 gaaggcccca gaacgtcttc ccggccgcca cgtcggcggc cgtcgcgccg ttcacgttgt
     4561 cggccgcggg ggccaccgcc atgatctcgt ccgtcgtctt cgaggccgcc gccgtcggcc
     4621 cggcagcggg ctccgtgaag gtcgcccccc gcttcacgcc cgccgcgccg ctcaggagcc
     4681 gctggtagac gtcccccagc gtgaaccgcg cgctcgccgc gtcgttcggc cccgcaggag
     4741 gcgccagctg cgcctcgatc atccctggcg ccaggaggac gaaggccaca atcaccacca
     4801 gcccgaccac gctcgcccgc ttctctcggt tcatccttct ctccttcgag acgcttcgct
     4861 ctcttccggt caccgccggc cggaggggtc ttcgtcagga gtcagccggc cgctcgcccc
     4921 ccggtcggtc gcgaccggga atcttgcctc gaagcgtcgc tcccgtccat cgctcgcgcg
     4981 tgagatccga accgtacaga gaccgaacac tccctacggg tcgaaggcgg aggcatccct
     5041 tcgccccggg gccggcaggc gacagcgagg agcgccatcg accacagacg agcgagtggg
     5101 ccgggatctc gccggctcca gctccgcgct cggcatctcc acgcgcgatg acgggaaggg
     5161 cgcacggctc gcgggccggt tcccatagaa tcgacgtatg tcgagtctcc cgaagcgacg
     5221 cgaggcgacg tacgaggacc tcctcgccct gcccgacgac gtcctcgggc agctcgtcga
     5281 gggcgagctt cacgccacac cgaggccggc gatcggtcac cagctctcgg tctcgatgct
     5341 cggaggcgag ctcgtgccgc cgtttcactc cggacgcggc ggccccggcg gctggtggat
     5401 cgtcgacgag ccggagctgc atctcggccg caacgtcctc gtccccgacc tcgccggctg
     5461 gcggagggag cggctcccgg tccttccccg cgaggcgttc ttcacgctcg cgcccgactg
     5521 ggtctgcgaa gtgctctcgc ccgcgacggc ccggatcgac cgcctgaaga agctcgcgat
     5581 ctacgcgcgc gagggcgtcg agcacgcctg gctcgtggat cccgccctcg agacgctcga
     5641 ggtcttccgg cggcagggcg aggcctggct cctcgtcctg acggccgggg gcgacgaggt
     5701 cgtgcgcgcc gagccgttcg acgcgataga gctctcgctc acggggctct ggatccggcc
     5761 ggcctgaagg gaaagggaac ggcgcggcct caggcccgac gacgccgtgg cattcgtcac
     5821 ggtgacggag ggcggcgacg gccgtccgca aggctgcggc cgccggttgc gagggcgcgg
     5881 gcacggctcc tttcgatcgg ccgcaccaca acctttcgat tggccgcacc ataacattac
     5941 gattagtcgc accctgacgt tacaatcagc cgatgctcga ggtcgacctc tacccgcgct
     6001 gcgccgaggc gcgcctggtc gaagcgctca ccgattcgcc ggtggtcctc ctccacgggc
     6061 ctcggcagag cggcaagacg accctggcgc gggtcgttgg cgaccgcctg ggctacgcct
     6121 acgtcagctt cgacgacgac gtcgcgcgcg gaggcgccga gtccgacccg gcgggtttcg
     6181 tcgccgacct gcccgatcgg acgatcctcg acgaagtgca gcgggtgccg gctctcttca
     6241 ccgcgctgaa gacggcggtc gaccgcaggc gcgtgccggg ccgcttcatc ctgacgggct
     6301 cggcgaacgt cctcctcgtg ccgaggatct cggactcgct ggccggccgc atggagatcc
     6361 tgcggttgca ccctctcgcc cagtccgagc tggagcggcg ctcctccgcc ttcctcgagg
     6421 cactcttcgg gcgcggcttc gaggttcggc agaccgaacg gctcggccgg cagctcgccg
     6481 agaggatcgc cgcgggaggg tatcccgccg cgctcgctcg accgagctcg cgccggcgca
     6541 ccgcctggta tcgcgactac ctcgacgcac tcgtccagcg cgacgtgcgc gacctggcac
     6601 gcatcgggtc gttcgacgcc ttgccgcgac tcctcgcgct ggcggcctcg cagacggcgc
     6661 ggctcctgaa cgtgacggat ctggcgtcgc ccttccagct gagtcggccg acgatccgcg
     6721 attacgtcac gcttctcgaa cgggtcttcc tcgtggacac gctgcccccc tggcacagca
     6781 accggctgag ccgactcgtg aagacgccga agctgcacgt cggcgacacg ggcctcgcgt
     6841 gcgccctgct cgggacggac ggtcccgccc tcgcggccga tcggcctctt ctgggccagc
     6901 tcctggagac gttcgtcttc caggagctgc ggcggcaggc gagctgtcgc gacgagccgc
     6961 tcacgttctc ccactaccgc gacaaggacg gcgccgaggt ggacgtcgtc atcgagcgca
     7021 gcgcgtggga gctggccgga gtggaggtca aggcggcggc cacggtgaag gatgccgact
     7081 tccgcggcct gcgaaggctg cgggcggccg caggcgaccg cttcgccgga ggcgtcgtgc
     7141 tctacgacgg ggagatcagc gcgggcttcg gcgatcggct ccacgccgtg ccgctgcgcg
     7201 ccctctggga aacgacctga gctcggggga cgaggtcgtg cgcgccgagc cgttcgacgc
     7261 gatcgagctc tcgcttaccg ggctctggat caggccggcc tgaagcccgt cttcagcggc
     7321 ctggccgcgg caggacgatc tctcatggac gaaaggcggg ttcaggcccc gcttctccct
     7381 ctgcgcgtga gaggagcctc gcgcctggac tcgggatccg aatctccgtt ccgcacgtcc
     7441 tcgtcgagac gctcggcggc gagatggagg atgtcggcga gctgtcgaat gacggcggcc
     7501 cccgatccgg gcgggcgtgg tggcctaccg gatgcccagc gggcgtgacc cgtggacggc
     7561 ggcgccggga agtccaggcc ggtcgcggcg tttgatacac ccgctaatat gtatggcgag
     7621 gtgtatgaat gaggacgaat atcgttctgg acgacgagct cgtccgcgag gccagccgcc
     7681 tcacgggtat ccggacgaag cgttccctcg tcgacgaggc tctccggctc ctcgtgagga
     7741 cgaagaagcg ccggtcgatc ctcgccctga agggaaagat ccggctcgcc gaggggtacg
     7801 accacaaggc gcttcgggag cggggccttg gtcctcgttg acagctcggt cctcgtcgac
     7861 ttcctccggg gccgggagac ggacgccacg cggaggctcg aggcgatcga gcggcaggag
     7921 gagtcgatcg ggtgggccct cccggtcgtc tgccttca
//
LOCUS       DAOTEZ010000177         7844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_6559_length_7844_cov_6.838747, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000177 DAOTEZ010000000
VERSION     DAOTEZ010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7844
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6559_length_7844_cov_6.838747"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="P5164_10515"
     CDS             <1..461
                     /locus_tag="P5164_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY44369.1"
                     /translation="ERMTKAQMEMTLDVMAHSLVYWVQDLAAAELLGAGAHVFAMTSA
                     GGHRIWPSYGAVSAAKAALESHCRQLAVELVPRGIAVNAIRAGVTDTPALRKIPGNVE
                     MIERVLAVHPAGRLTTPQDVANVIVAMARPETAWMTGNVLGVDGSEDLLG"
     gene            458..1267
                     /locus_tag="P5164_10520"
     CDS             458..1267
                     /locus_tag="P5164_10520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY44370.1"
                     /translation="MISLPAGAYALVTGASAGIGEEIARSLARRKVPQLLVARSAEKL
                     SALAAELRSASGVAVEAIPLDLEADGAAERLVAATEGAGKPVGLLVNNAGFGWYGPQS
                     EQGLERTLRMLRLNVAALVELTDRLLPPMRARGRGLVLNVASTAAFLPVPYMSVYAAT
                     KAFVLSYSQALHEELTGTGIVVTALCPGTTRTDFHRVAGLAPGERVRFPSLSAETVAE
                     AGLRGLARGRAVVVPNLLDSAWIFTGRLVPRAIPPKIAAALFSRLKRAPRD"
     gene            complement(1285..2184)
                     /locus_tag="P5164_10525"
     CDS             complement(1285..2184)
                     /locus_tag="P5164_10525"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRY44371.1"
                     /translation="MRSGAAVASLLDEALAGLWRARRMTAVSVLQIGFSLFLVGLFLL
                     AAENLGGVVDALREETAVTVFLRPGADEAAQADLERRALDSKLVAAVRRVSPEEARRR
                     FARSFASLAGATGTLSSNPFPSSVELDLLPGAASSAALRPFLANLAAFPAVEEVQFDV
                     DWVRRLRGVVRLVAATGAATGFCLALGAAFTIANVVRLTILLHRDEIDILRLVGAPEI
                     LIRGPFLLGGVLQGLLGGLLALGLLGLAFRGVLMFITATENVLLGVFVVRFLSPATCG
                     LLVLGGVLAGLVGGTIASRRHHV"
     gene            complement(2181..2918)
                     /gene="ftsE"
                     /locus_tag="P5164_10530"
     CDS             complement(2181..2918)
                     /gene="ftsE"
                     /locus_tag="P5164_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HRY44372.1"
                     /translation="MIEFYGVSKEYHGRRVLKNLSFQVAQGEFVFLTGPSGAGKTTVL
                     KLLYRAETPGAGQILVNGRNVVSMPPSRIPELRRTMGIVFQDYKLLPTRTVFENVSFV
                     LKFLGVPLAERRRRAYQVLRLVELHHRVNAFPEELSGGEQQRVALARALVNEPELLVA
                     DEPTGNLDHRLAQEMMRLFSQINVRGTTVLVATHDVALIRETGRRCLTLDRGGVLEGL
                     VEAALPPQAAWRREGSSAHPVVPEERP"
     gene            complement(2915..4375)
                     /gene="gatB"
                     /locus_tag="P5164_10535"
     CDS             complement(2915..4375)
                     /gene="gatB"
                     /locus_tag="P5164_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004873193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRY44373.1"
                     /translation="MLPVDLEAVIGLEVHVQLKTRTKAFCACPVVFGAAPNGAVCPVC
                     LGYPGALPVPNREMAALGARLALATGCDVHARSAFARKSYFYPDLPKGYQITQFDRPL
                     ATGGRIDVRGTDGTARAVRLRRVHLEEDAGKSIHDAQGEDVPPGVTLVDLNRAGTPLA
                     EIVTEPDLRTPSEAADFLSRVRRLVRWIGVSDGSMEEGSLRCDANVSLRPAGAEEPGT
                     RVEVKNLNSIAHVRKALEHEVSRQAEALRAGRAVAADSRLFDPASGSTRPMRGKEESE
                     DYRYFPDPDLGPLVLDGAWLEELAASLPEPPEAREARLVAQLGLSPADAATLCATRPL
                     ADAFEEAVALHPSNARGVASWFLGGLLARMGDSARQEGRLPVAPAAIARLVARIDDGT
                     VSRRIAAELVDLLCAGAGDVDGLIRERGLFQVTDEGPIRAAVDAVLAANPALAAACRG
                     GKSASFAWFVGQVMKATGGKASPAVVNRLLRERLDA"
     gene            4463..5425
                     /locus_tag="P5164_10540"
     CDS             4463..5425
                     /locus_tag="P5164_10540"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRY44374.1"
                     /translation="MTRARSIRTPETGGRPAGPPVVSRLTHATFGLWFPFFRWLVTAF
                     PPEWVARLAAATAERAMWGRENVREAILENHAAVLGLPPWRREVEESAREMLGHHSRS
                     WIDLLRFSARPPTDLEAVVPERVGTEHLLAARDAGRGAILLTAHVGNFEIGGFFLQAV
                     GLKVAVVYLPDPSPVVERDRTAARDRLGIRSIPLVSELSAVKVLRSLEEGYFVAIQGD
                     RDYGGTGRKMPFLGREVSFPVGPFRIAAAAGVPLLPVFVLRGEGGRYRTVVEPPIRVD
                     AARRGERDEAERRAMTDFVAILERTIRDNHAQWYTFARFWEPPA"
     gene            5505..6344
                     /locus_tag="P5164_10545"
     CDS             5505..6344
                     /locus_tag="P5164_10545"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRY44375.1"
                     /translation="MKIVLAIDPAQPLAAYRDALLAAGARDEEIVVVQPGDALPGSFD
                     GLLVSGGADVDPSRYGETKVNDTVTPRPERDALDFALVERAAGLGAPVFGICRGLQVL
                     NVALGGTLWQDLPSQRDRGVAHAFSTRDGHDPAHLAHVVRARRGAPAGLPLAAEISAL
                     DGEPVNSRHHQAVKDLAPGLVPLAAAPDDLPEAFARAEGPFLAAVQWHPENLVDRPAQ
                     KALFEAFLEAARRRAARSNPHASAGEHADGVTSGPRGHQHASGEPRGPSDARDTAGVP
                     EPR"
     gene            6341..7318
                     /locus_tag="P5164_10550"
     CDS             6341..7318
                     /locus_tag="P5164_10550"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY44376.1"
                     /translation="MTKPRLALLALLLLLAVPAELALAQASSAPSGPPPLPKDVPAFQ
                     VGQYNAYTWYGRFGYTNLSFIDTGDGVLVVDTGWTKQDGENLKAQIAEKTKGKPIRWI
                     VMTQTDVDSNGGIAAFLPTDATIFVHARALDPLSAGVLRAEPGQKKPTVVGVADHLVL
                     NAGGRRFELVAAPGRAHSEYDLVLYNNDSGLVWAGDLVTPGRCPNLTNPASDPNGWLG
                     MIGRIQSLGAVGIVPTRGDATKAVADEIEKSRAYIERVLRFVAEQKVKNSPEARVAAE
                     LSTKKLGDYCPVNGDNANVIGLYRRMQADGTFRFPGAAVPARPAAPAPR"
     gene            complement(7548..>7844)
                     /gene="queF"
                     /locus_tag="P5164_10555"
     CDS             complement(7548..>7844)
                     /gene="queF"
                     /locus_tag="P5164_10555"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HRY44377.1"
                     /translation="TITIPEFTSICPKTGLPDFATLVVAYVPDTACVELKSFKEYLLA
                     YRNLGIFNENVVNRVLADLVAATRPRKMSVRGVFTPRGGIQTTVEARWPKPARP"
BASE COUNT         1094 a         2838 c         2897 g         1015 t
ORIGIN      
        1 aggagcggat gacgaaggcg cagatggaga tgacgctcga cgtcatggcc cactccctcg
       61 tctactgggt gcaggacctc gccgcggcgg agctcctcgg cgccggcgcg cacgtcttcg
      121 cgatgacctc cgccggcggg caccggatct ggccctcgta cggcgcggtc tccgccgcga
      181 aggcggctct cgagagccac tgccgccagc tcgcggtgga gctcgtcccg cgcgggatcg
      241 ccgtgaacgc gatccgcgcc ggcgtgaccg acacgccggc cctccggaag atccccggga
      301 acgtcgagat gatcgagcgg gtcctcgcgg tccacccggc cggacggctg acgactccgc
      361 aggacgtcgc gaacgtgatc gtcgcgatgg cgcggcccga gacggcgtgg atgacgggga
      421 acgtcctcgg cgtggacggc tccgaggacc tcctcggttg atctccctcc ccgcgggggc
      481 gtacgcgctc gtcaccggcg cctcggccgg gatcggcgag gagatcgcgc gctcgctcgc
      541 gcggaggaag gtcccgcagc tcctcgtcgc ccgctcggcg gagaagctct ccgcgctcgc
      601 ggcggagctg cgctcggcct ccggcgtcgc ggtggaggcg atcccgctcg acctcgaggc
      661 ggacggcgcc gcggagcggc tcgtcgccgc gacggaagga gccgggaagc ccgtcggcct
      721 cctcgtgaac aacgcgggct tcggctggta cggcccgcag tccgagcagg ggctcgagcg
      781 gactctccgg atgctccggc tgaacgtcgc cgcgctcgtc gagctgaccg accggctcct
      841 ccccccgatg cgcgcgcgcg gacgggggct cgtcctgaac gtcgcctcga ccgccgcgtt
      901 cctccccgtc ccctacatgt cggtctacgc cgcgacgaag gcgttcgtcc tctcctactc
      961 gcaggcgctc cacgaggagc tcacggggac ggggatcgtc gtgacggcgc tctgccccgg
     1021 gacgacgcgg accgacttcc accgcgtcgc cggcctcgcg ccgggggagc gtgtccgctt
     1081 tccgtcgctc tcggccgaga cggtcgccga ggcggggctc cgcggcctgg cgcgcggccg
     1141 ggcggtcgtc gtcccgaacc tcctcgactc cgcctggatc ttcaccggtc gcctcgtccc
     1201 gcgcgcgatt cccccgaaga tcgcggccgc cctcttctct cggctgaagc gcgcgccgcg
     1261 agactgaacc ccgccgtcgc ggcgtcagac gtggtggcgc cgcgaggcga tggtcccgcc
     1321 gacgagcccc gcgaggacgc cgccgaggac gaggaggccg cacgtcgccg gcgagaggaa
     1381 ccggacgacg aagacgccga ggaggacgtt ctccgtcgcg gtgatgaaca tcaggacgcc
     1441 gcggaacgcg aggccgagca ggccgagcgc gaggagccct ccgaggagcc cctggagaac
     1501 gccgccgagg aggaacggcc cgcggatgag gatctccggg gcgccgacga ggcggaggat
     1561 gtcgatctcg tcgcggtgga ggaggatcgt cagccggacg acgttcgcga tcgtgaaggc
     1621 ggcgccgagc gcgaggcaga agccggtcgc cgcccccgtc gccgcgacga ggcggacgac
     1681 gccccgcagg cgtctcaccc agtcgacgtc gaactgcacc tcctcgacgg cggggaacgc
     1741 cgcgaggttc gcgaggaagg ggcggagggc ggcggaggac gcggctcccg ggaggaggtc
     1801 cagctcgacc gaggagggga aggggttcga ggagagcgtc ccggtggctc cggcgaggct
     1861 cgcgaaggac cgggcgaacc ggcgtcgcgc ctcctcgggg gagacgcggc ggacggcggc
     1921 gacgagcttc gagtcgagcg cgcggcgctc gaggtcggcc tgcgccgcct cgtcggcgcc
     1981 gggccggagg aagacggtga ccgcggtctc ctcgcggagc gcgtcgacga cgccgccgag
     2041 gttctcggcg gcgaggagga agagcccgac caggaagagg gagaagccga tctggaggac
     2101 ggagaccgcc gtcatccggc gcgcgcgcca gaggccggcg agcgcctcgt ccaggagcga
     2161 ggcgaccgcg gcgccgctcc tcacggccgc tcctccggga cgaccgggtg cgccgacgag
     2221 ccctcgcgcc gccacgccgc ctgcggcggg agcgccgcct cgacgagccc ctcgagcacg
     2281 ccgcctcggt cgagcgtgag acagcggcgg ccggtctcgc ggatcagcgc gacgtcgtgc
     2341 gtcgcgacga ggacggtcgt ccctcggacg ttgatctgcg agaagagccg catcatctcc
     2401 tgcgcgaggc ggtggtcgag gttcccggtc ggctcgtcgg cgacgaggag ctcgggctcg
     2461 ttcacgaggg cgcgcgccag ggcgacccgc tgctgctcgc cgccggagag ctcctcggga
     2521 aacgcgttga cgcggtggtg gagctcgacg agccggagga cctggtacgc ccggcggcgc
     2581 ctctcggcga gcgggacgcc gaggaacttc aggacgaacg agacgttctc gaagaccgtg
     2641 cgcgtcggga ggagcttgta gtcctggaag acgatcccca tcgtccggcg gagctccggg
     2701 atgcgcgacg gcggcatcga gacgacgttc cgcccgttga cgaggatctg cccggcgccc
     2761 ggcgtctcgg cgcggtagag gagcttgagg acggtcgtct tccccgcgcc cgaagggccg
     2821 gtgaggaaga cgaactcccc ctgggccacc tggaacgaga ggttcttcag cacgcgccgg
     2881 ccgtggtact ccttcgagac gccgtagaac tcgatcacgc gtcgagcctc tcccggagga
     2941 ggcggttcac gacggcgggg ctcgccttcc cgccggtcgc cttcatcacc tgtccgacga
     3001 accaggcgaa cgacgcgctc ttccccccgc ggcaggcggc ggccagcgcc gggttcgccg
     3061 cgaggaccgc gtcgacggcg gcgcggatcg gcccctcgtc cgtcacctgg aagagccccc
     3121 tctcgcggat cagcccgtcg acgtcccccg cgcccgcgca gaggaggtcg acgagctccg
     3181 cggcgatcct ccgcgagacg gtcccgtcgt cgatccgcgc cacgagccgg gcgatcgcgg
     3241 ccggagcgac gggaagacga ccctcctgcc gcgcggaatc gcccatccgc gcgaggaggc
     3301 cgccgaggaa ccaggaggcg acgccgcgcg cgttcgacgg gtggagcgcg acggcctcct
     3361 cgaacgcgtc ggcgagcggg cgcgtcgcgc agagcgtcgc cgcgtcggcc ggggagaggc
     3421 cgagctgggc gaccagccgc gcctcgcgcg cctccggcgg ctcggggaga gaggcggcga
     3481 gctcctcgag ccacgcgccg tcgaggacga gggggccgag gtcggggtcg gggaagtagc
     3541 ggtagtcctc ggactcctct ttcccgcgca tcggtcgcgt cgagccggac gcggggtcga
     3601 agaggcggga gtcggcggcg acggctctgc cggcgcggag ggcctccgcc tggcgcgaga
     3661 cctcgtgctc gagcgccttc cgcacgtggg cgatcgagtt gaggttcttg acctcgaccc
     3721 gggtcccggg ctcctccgcg ccggccgggc ggagggagac attcgcgtcg cagcggaggg
     3781 agccctcctc catgcttccg tccgagacgc cgatccaccg aacgaggcgc ctcacgcggg
     3841 agaggaagtc ggcggcctcg gagggggtgc ggagatcggg ctcggtgacg atctcggcga
     3901 gcggggtccc ggcccggttc aggtcgacga gggtgacgcc gggagggacg tcttccccct
     3961 gcgcgtcgtg gatcgacttg cccgcgtcct cctcgaggtg gaccctccgg aggcggacgg
     4021 ctcgggccgt gccgtccgtg ccgcggacgt cgatccggcc gccggtggcg agcgggcggt
     4081 cgaactgggt gatctgatat cccttcggca ggtcggggta gaagtagctc ttccgggcga
     4141 aggcggagcg cgcgtggacg tcgcagcccg tggcgagcgc gaggcgcgcc ccgagcgccg
     4201 ccatttcgcg gttcgggacg gggagggctc ccgggtagcc gaggcagacc gggcagacgg
     4261 cgccgttcgg cgccgctccg aagacgaccg ggcaggcgca gaacgccttg gtccgcgtct
     4321 tcagctgaac gtggacctcc agcccgatga ccgcctcgag atcgacaggg agcattccgg
     4381 acggcgagtc tagccgagcg cgccgccgtc gctcgctgcg tctagaatcg acgccccgtt
     4441 gcgccgcgag gcgctccttc ccatgacacg agcccgatcc atccgaacgc ccgagacggg
     4501 cggccgcccc gccgggccgc ccgtcgtctc gcgcctgacc cacgcgacgt tcgggctctg
     4561 gtttccgttc tttcgctggc tcgtgaccgc cttccctccc gagtgggtcg cgcgcctggc
     4621 ggcggcgacg gcggaacggg cgatgtgggg acgcgagaac gtgcgggagg cgatcctcga
     4681 gaaccacgcg gcggtcctcg gcctcccgcc gtggcggcgc gaggtggagg agtcggcccg
     4741 cgagatgctg gggcatcact cgcgctcctg gatcgacctc ctccgcttct cggcccggcc
     4801 cccgacggac ctcgaagccg tcgtcccgga gagggtcggc accgagcacc tcctcgccgc
     4861 gcgcgacgcg gggcggggcg cgatcctgct caccgcccac gtcggcaact tcgagatcgg
     4921 cgggttcttc ctgcaggccg tcggactgaa ggtcgcggtc gtctacctgc ccgacccgtc
     4981 gcccgtcgtg gagcgcgacc ggaccgcggc gcgcgaccgg ctcggcatcc ggtcgatccc
     5041 gctcgtctcg gagctctcgg ccgtgaaggt cctgcggagc ctcgaggagg ggtatttcgt
     5101 cgcgatccag ggggaccgcg actacggcgg gacggggcga aagatgccgt tcctcgggcg
     5161 cgaggtctcg ttccccgtcg ggccgttccg gatcgccgcg gccgccgggg tcccgctcct
     5221 gcccgtcttc gtcctccgcg gagaaggagg ccggtaccgg acggtcgtcg agccgccgat
     5281 ccgggtcgat gcggcgcggc gcggagagcg ggacgaagcc gagcggcggg ccatgaccga
     5341 cttcgtcgcg atcctggaac ggacgatccg cgacaaccac gcccagtggt acaccttcgc
     5401 ccgcttctgg gagccgccgg cctgaagtcg gagcgcgcct ggaacgggcc ttgctagact
     5461 tcgccgccgc cgtcgcccgc cgccggatcg gcccgaagca ggaaatgaag atcgtcctcg
     5521 caatcgaccc cgcgcagccg cttgcggcct accgcgacgc cctcctcgcg gccggagcgc
     5581 gcgacgagga gatcgtggtc gtgcagccgg gagacgcgct tcccggctcc ttcgacggcc
     5641 tgctcgtctc gggcggagcg gacgtcgacc cgtcgcggta cggggagacg aaggtgaacg
     5701 acacggtgac gcccaggccg gagcgggacg cgctcgattt cgcgctcgtg gaacgggcgg
     5761 ccggcctcgg agcccccgtc ttcggcatct gccgcggcct gcaggtcctc aacgtcgcac
     5821 tcggcggaac gctctggcag gacctcccgt ctcagcgtga ccggggcgtc gcgcacgcgt
     5881 tctcgacgcg ggacgggcac gacccggcgc acctcgcgca cgtcgtccgt gcccggcggg
     5941 gcgctcccgc cggcctgccg ctcgccgcgg agatctcggc gctcgacggc gagccggtca
     6001 actcccgcca ccaccaggcg gtgaaggacc tcgctcccgg cctcgtcccc ctcgcggccg
     6061 cgcccgacga cctcccggag gcgttcgcgc gcgccgaagg tccgttcctc gccgccgtcc
     6121 agtggcaccc cgagaacctc gtcgaccggc cggcgcagaa ggccctcttc gaagccttcc
     6181 tcgaagcggc ccgccgccgg gcggctcgat cgaaccccca cgcctctgcc ggggagcacg
     6241 ccgacggcgt cacctccggg ccgagaggcc accaacacgc ctccggcgag ccgcgcggac
     6301 cctccgacgc ccgcgacacc gccggggtgc cggagccaag atgacgaagc cccgcctcgc
     6361 ccttctcgca ctgctcctcc tcctcgccgt tccggcggag ctcgccctcg cgcaggcgtc
     6421 cagcgcgccg tccggaccgc cgccgctgcc gaaggacgtc cccgcgttcc aggtggggca
     6481 gtacaacgcc tacacctggt acgggcgctt cggctacacg aacctgtcgt tcatcgacac
     6541 gggggacggc gtcctcgtcg tcgacacggg ctggacgaag caggacggcg agaacctgaa
     6601 ggcgcagatc gccgagaaga cgaaggggaa gccgatccgc tggatcgtga tgacgcagac
     6661 ggacgtcgac tcgaacggcg ggatcgcggc gttcctcccg accgacgcga cgatcttcgt
     6721 ccacgcccgc gcgctcgacc cgctctcggc cggcgtcctg cgggccgagc cggggcagaa
     6781 gaagccgacc gtcgtcggcg tggcggacca cctcgtcctg aacgccgggg ggcgccggtt
     6841 cgagctcgtc gcggcgccgg ggcgggccca ctcggagtac gacctcgtcc tctacaacaa
     6901 cgacagcggc ctcgtctggg caggggacct cgtcacgccc gggcgctgcc cgaacctgac
     6961 gaaccccgcc tccgacccga acggctggct cgggatgatc ggccggatcc agtcgctcgg
     7021 cgccgtcgga atcgttccca cgcgcggcga cgcgacgaag gccgtcgccg acgagatcga
     7081 gaagagccgc gcctacatcg agcgggtcct ccgcttcgtc gccgagcaga aggtgaagaa
     7141 ctccccggag gcgcgcgtgg ccgcggagct ctcgacgaag aagctgggcg actactgccc
     7201 ggtcaacggc gacaacgcga acgtcatcgg actctaccgg cggatgcagg ccgacggcac
     7261 cttccggttc ccgggcgcgg ccgtccccgc gcgtcccgcc gccccggctc cgcgctgaga
     7321 agaaaggccc gaaaacgaaa atcccggccg gttgatcatg ccggccggga cgggacggga
     7381 gggcgtcggg tcctccgaac tcacgttcgc cctcttcctt ttgagccgcg tccgcggccc
     7441 gtctttcctt cgcggggccg atccggtgtc gccccggaaa gaaccgcgtt accgctggaa
     7501 agagccttcg tgtcccgcag aaaacatggg cttcggaacg cgcgctgtca agggcgcgcg
     7561 ggcttcggcc agcgcgcctc gaccgtcgtc tggatgccgc cgcgcggcgt gaagacgccg
     7621 cgcacggaca tcttccgcgg ccgcgtcgcg gcgacgaggt cggcgaggac gcggttcacg
     7681 acgttctcgt tgaagatgcc gaggttccgg taggcgagga ggtactcctt gaacgacttc
     7741 agctccacgc acgccgtgtc ggggacgtac gcgacgacga gcgtcgcgaa gtcggggagt
     7801 ccggtcttcg ggcagatcga ggtgaactcc gggatcgtga tcgt
//
LOCUS       DAOTEZ010000178         7840 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_6565_length_7840_cov_6.865254, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000178 DAOTEZ010000000
VERSION     DAOTEZ010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7840
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6565_length_7840_cov_6.865254"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="P5164_10560"
     CDS             complement(<1..1039)
                     /locus_tag="P5164_10560"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRY44378.1"
                     /translation="MSKRFSDLDQKRRELLLGGGAERIEKQHRQGKLTARERLALLLD
                     PDSFVEFGLWAQEDNAEPGAKVLAAEGVVTGKGWVDGRAVYAFSQDFTVSGGSVGKIH
                     AAKICECLRGALRTGLPVVGFNDSGGARIQEGVEALSGYGQIFYTNTLLSGVVPQISV
                     IAGPCAGGAAYSPALTDFIVMVEESAKMFIAGPDVIKAATGEAITDEALGGAKVHAEV
                     SGNIHFAAPDERSAVAIVKRLLSYLPSNNLESAPDLPFEQPIVDDERMDDIVPEDPRK
                     AYDVKDVIARLVDPASFLEVQEAFGRSIVTGFARLGGMVVGLVANQPMHLAGSLDMDA
                     SDKAARFVRTCN"
     gene            1168..2337
                     /locus_tag="P5164_10565"
     CDS             1168..2337
                     /locus_tag="P5164_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013018642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HRY44379.1"
                     /translation="MDRVVDFAALSAEDDRSPLVCSLCDAIEATWDEGLYPLVEVDRP
                     WSRHNPLLRGEFARPKAIRWYLERELGKLAAAGAKVTVGGSRERVPFDDPAHFGSLDE
                     EAWDLRQKKLFLFRAERIDLSLNRLEHYTGTPAERFQRHVLFTNYEMHAEAFRARYPG
                     AVGPARSVQMPAWHHELPGNAGLTLVNIGVGPSNAKTITDHVAVLRPDAVLMVGHCAG
                     LRNHQEVGDLVLATGYMRDDRILDDVLPTSVPVASNHFLNALLLEEMEARGLSYRLGT
                     VFTTANRNWEFNQSGALAAIRASRSVAVDMESATVAANGFRYRIPTAALLCVSDKPLH
                     GQPKLSAQAQAFYRESRIAHLEVAAEALEKARDRFPGGLPSSDIRSSDEPLLGSA"
     gene            complement(2377..3297)
                     /locus_tag="P5164_10570"
     CDS             complement(2377..3297)
                     /locus_tag="P5164_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002611332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HRY44380.1"
                     /translation="MARILVSGLVNLETTVKVDAFPVPYFPVRYPFFGVASSVSGVGY
                     NVARALQALGNAPLLLSLVAPDLAGRQVRDALSRDGLDGFWVRDGARETAQSVVLYDE
                     EGRRQIHVDLKDVQERAYPQELFVQALAACDAAALCNVNFSRPFLAAARSAGKLVATD
                     VHALSSLDDPYNGDFLRAAQVVFLSDDALPAPAEEFARDLGARFGPEVVVVGMAAKGA
                     LLHLPFEGIVERVPAVRTRSVVSTVGAGDALFSAFLDGYLAERDARAALRRAVVFASW
                     KLGAASAAEGFLGAEPLRQLVASDPDPYEP"
     gene            3388..4398
                     /locus_tag="P5164_10575"
     CDS             3388..4398
                     /locus_tag="P5164_10575"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRY44381.1"
                     /translation="MPPTHRRLASALLAFALLASPAAVLLAERGAASAVRSRRTVAVT
                     VDDLPFVAYGLPLEAVRKLSRELVASLAARKVPAVAFVNEDRLFVRGEVDERIALLTE
                     WLDAGMELGNHTFGHVGFTATPLPKMKDAVVQGEVVTRGLLARRGKTLRWFRHPFTQT
                     GPTKEAKDAFETFLAERGYTVAPFSIEHEDWVFASADAKLAAERDEAGRTRLLEAYLE
                     NFEARVAFYEERSRALFGREIPQIHLAHANALNARAMPYLLERLERRGYAFVTLDEAL
                     ADPAWKSPDGYVGKWGPSWLHRFAAARGEDVRALFRAEPEAPTWVAQLDEARRAAAPA
                     AP"
     gene            complement(4415..6154)
                     /locus_tag="P5164_10580"
     CDS             complement(4415..6154)
                     /locus_tag="P5164_10580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRY44382.1"
                     /translation="MESSSNGLRRRPGRIARATAAVATFVAVAAVVLPSGRTAAQPAS
                     DAPRIAASAEVSILGVDVVVTDRNGKPVHGLTAADFELRYGGKPVVITNLHEERSEET
                     GVLPGAPAGIDPSGAVPRGTEETVSRQPRKVVVFVDRLHLPEPTRRRELFEGVKAFLA
                     KALTGPGDEAMVVSWGDSVRVVRSFTADLAALDETLDVVARRVSRLDGQENEVAELAS
                     RDLWFDSIAADPRVGGTVGSTETSREIGTLQAYGMMKAKVTALRGLVALMAGMEGRKS
                     LVLVAHHFGRFPGREYHVGPRSAPVGSPRERDGKPLLEELAAAANASGVTLYGIYPYE
                     RVALASAADSAALNPSTSSAAYGSGFDAIWMNEMAGLDFVTKQTGGSTAGHPGELPRF
                     LDQVASDLGSYYSIGFPASGTTKPEAVRVRVKREGLAVRTRTTVAERTAEERLTDRAL
                     ANLFAPDASSRIGISVAAGEAKPEGKKYRIPLEVRIPLGALVRVPGPSAAKGKFSVFV
                     VAATPKGDFSEVVRRRQEFEVKRGEEAKADASHLTYTLEVESDAPAPRISLAVWDEIG
                     GEAGFALVGKRRG"
     gene            6260..7006
                     /gene="gpmA"
                     /locus_tag="P5164_10585"
     CDS             6260..7006
                     /gene="gpmA"
                     /locus_tag="P5164_10585"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HRY44383.1"
                     /translation="MYTLVLLRHGESQWNLENRFTGWTDVDLTEKGREEAREAGRLLR
                     EGGYDFDVAYTSVLKRAIRTLWIALDEMDRMWIPVIRSWRLNERHYGALQGLNKAETA
                     AKFGEEQVKVWRRSYSTPPPELTPDDERFPGHDRRYAALAKEELPLTESLKETVARAV
                     PYWESVIAPEVKAGRRVLVAAHGNSLRAMVKYLDGISDEEIVGLNIPTGVPLVYHLDE
                     NLKPLKSFYLGDAEAVAAKAAAVANQGKKG"
     gene            complement(7020..7604)
                     /locus_tag="P5164_10590"
     CDS             complement(7020..7604)
                     /locus_tag="P5164_10590"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRY44384.1"
                     /translation="MNPVNDPVRSFLELEKRLEREPEGSQGEIARGIYMMTPRPRAAH
                     GGAQVNLGAALRLRFGWGEGVVAPEWLFVAEPEVRSEENLSRLVPDLAAWRRSTTGWP
                     DLNITPATLAPEWVAEVLSPTTERFDRREKMGAWGAMGVGYVWLVDVDRRRIETFANV
                     RGTMVAGTVLEADAELAAEPFGALGLPVARLFPG"
BASE COUNT         1159 a         2885 c         2718 g         1078 t
ORIGIN      
        1 tgttgcacgt gcggacgaaa cgggccgcct tgtcggacgc gtccatgtcg agcgagccgg
       61 cgagatgcat cggctggttc gcgacgaggc cgacgaccat cccgccgaga cgggcgaagc
      121 ctgtgacgat gctccgcccg aacgcctcct gcacctcgag gaacgacgcc gggtcgacca
      181 ggcgggcgat gacatccttc acgtcgtacg ccttccgggg gtcctcggga acgatgtcgt
      241 ccatccgctc gtcgtcgacg atcggctgtt cgaacgggag gtccggggcc gactcgaggt
      301 tgttcgaggg gaggtacgac aggagccgct tcacgatcgc gacggccgac cgctcgtcgg
      361 gcgccgcgaa gtggatgttc cccgagacct ccgcgtggac cttcgccccg ccgagcgcct
      421 cgtcggtgat ggcctcgccg gtcgccgcct tgatgacgtc gggcccggcg atgaacatct
      481 tcgcgctctc ctcgaccatg acgatgaagt cggtgagcgc cggcgagtag gccgcgcccc
      541 ccgcgcaggg gccggcgatg acgctgatct gcgggacgac gccggagagg agcgtgttcg
      601 tgtagaagat ctgtccgtat ccggagaggg cctcgacccc ctcctggatc cgggcgccgc
      661 ccgagtcgtt gaagccgacg acggggagcc cggtccggag ggccccgcgc aggcactcgc
      721 agatcttcgc ggcgtggatc ttgccgacgg agccgccgct gacggtgaag tcctgggaga
      781 aggcgtagac ggcccggccg tccacccacc cctttccggt gacgaccccc tcggccgcga
      841 ggaccttcgc gcccggctcg gcgttgtcct cctgggccca gaggccgaac tcgacgaagc
      901 tgtcggggtc gaggaggagc gcgaggcgct cgcgcgccgt cagctttccc tggcggtgct
      961 gcttctcgat ccgttcggcc ccgccgccca gaagaagctc ccgcctcttt tgatcgaggt
     1021 ccgaaaaacg cttggacatg tgcctccttg gtactccttc cggctggaag ggcgaggaat
     1081 cgtactcctc cgggagccgg cctgccgggg cgcgtccttc ggtgccgacg tgcggagaga
     1141 atgcgtctcc ggtaatctgg gtgtttcatg gatcgcgtcg tcgatttcgc cgccctctcc
     1201 gccgaggacg atcggtctcc gctcgtctgc tcgctctgcg acgcgatcga ggcgacctgg
     1261 gacgaggggc tctacccgct cgtcgaggtg gaccgcccct ggtcgcgcca caaccccctc
     1321 ctccgcggcg agtttgcccg cccgaaggcc atccggtggt acctcgagcg agagctcggg
     1381 aagctggcgg cagccggcgc gaaggtcacc gtcggcggct cgcgcgagcg agtcccgttc
     1441 gacgacccgg cgcatttcgg gagcctcgac gaggaagcct gggacctccg gcagaagaag
     1501 ctcttcctct tccgcgccga gaggatcgac ctctccctga accgcctcga gcattacacc
     1561 ggcacccctg cggaacggtt tcagaggcac gtcctcttca cgaactacga gatgcacgcc
     1621 gaggcgttcc gcgcccgcta tcccggcgcc gtcggcccgg cgcggagcgt ccagatgcct
     1681 gcgtggcacc acgagctgcc gggcaatgcc gggctcacgc tcgtgaacat cggcgtcggc
     1741 ccctcgaacg ccaagacgat caccgaccac gtcgccgtcc tccgccccga cgccgtcctg
     1801 atggtgggcc actgcgccgg cctccgaaac caccaggagg tcggcgacct cgtcctcgcg
     1861 accggctaca tgagggacga ccggatcctc gacgacgtcc tgccgacgag cgtcccggtc
     1921 gcctcgaacc acttcctgaa cgcgctcctt ctcgaggaga tggaggcgcg cggcctttcc
     1981 taccggctcg ggacggtctt cacgaccgcg aaccggaact gggagttcaa ccagtcgggc
     2041 gccctcgccg cgatccgcgc gagccggagc gtcgccgtcg acatggagtc ggcgaccgtc
     2101 gccgcgaacg gcttccgcta ccgcatcccg acggccgctc tcctctgcgt gagcgacaag
     2161 ccgctccacg gccagccgaa gctctccgcc caggcccagg cgttctaccg cgagagccgg
     2221 atcgcccacc tcgaggtggc ggccgaggcg ctcgaaaagg cgcgcgaccg tttccccggc
     2281 ggccttccct cctccgacat ccgctcttcg gacgagccgc tcctcgggtc ggcctgaggc
     2341 gcggcgcgct tccgcgcgac cgcggggcgt ccgcggctac ggctcgtacg ggtccgggtc
     2401 ggacgcgacg agctgacgga gcggctcggc gccgaggaag ccttccgcag ccgaggccgc
     2461 cccgagcttc caggacgcga agacgaccgc gcggcggagc gccgcgcgcg cgtcgcgttc
     2521 cgcgaggtag ccgtcgagga acgccgagaa gagcgcgtcg cccgcgccga ccgtagagac
     2581 gacggagcgt gtcctcacgg ccgggaccct ctcgacgatc ccttcgaacg ggaggtggag
     2641 gagcgcccct ttcgccgcca tcccgacgac gacgacctcg gggccgaacc gcgcgccgag
     2701 gtcccgcgcg aactcctcgg ccggcgccgg gagcgcgtcg tcgctcagga agacgacctg
     2761 cgcggcgcgg aggaagtcgc cgttgtacgg gtcgtcgagg gaggaaaggg cgtggacgtc
     2821 ggtcgccacg agcttgcccg ccgaccgcgc cgcggcgagg aaggggcgcg agaagttgac
     2881 gttgcagagg gccgccgcgt cgcacgcggc gagcgcctgg acgaagagct cctgcgggta
     2941 ggcccgctcc tggacgtcct tcaggtcgac gtggatctgg cgtcgcccct cctcgtcgta
     3001 gaggacgacc gactgcgccg tctcgcgggc cccgtcgcgg acccagaagc cgtcgaggcc
     3061 gtcccgcgac agggcgtccc ggacctggcg ccccgcgagg tcgggggcga cgagcgagag
     3121 gagaagaggc gcgttcccga gagcctgaag agcccgggcg acgttgtagc cgacgccgga
     3181 gaccgaggag gcgacgccga agaacgggta gcggaccggg aagtacggca ccgggaaggc
     3241 gtcgaccttc acggtcgtct cgaggttcac gaggccggag acgaggattc gagccatgag
     3301 gccccattct gctccagggc gcccttgggc gcggacggca cgtgtgcaaa atgcacggcg
     3361 accccatctc gcgacccgga ggttcccgtg ccccctacgc atcgccgcct cgcatccgcc
     3421 ctcctcgcct tcgccctgct cgcctctccg gccgccgtcc tcctcgcgga gaggggcgcc
     3481 gcctccgccg tccgcagccg ccggaccgtc gccgtcacgg tcgacgacct cccgttcgtc
     3541 gcctacggcc tcccgctcga ggcggtccgg aagctctcaa gggagctcgt cgcctcgctc
     3601 gccgcccgga aggtccccgc cgtcgccttc gtcaacgagg accgtctctt cgtccgcggc
     3661 gaggtggacg agcggatcgc cctcctgacg gagtggctcg acgccgggat ggagctcggg
     3721 aaccacactt tcggccacgt cggcttcacc gcgacgcccc ttccgaagat gaaggacgcc
     3781 gtcgtccagg gggaggtcgt cacgcgcggg ctcctcgccc gccgggggaa gacgctccgc
     3841 tggttccgtc acccgttcac gcagacgggt ccgacgaagg aggcgaagga cgccttcgag
     3901 acgttcctcg cggagcgcgg ctacaccgtc gcccccttca gcatcgagca cgaggactgg
     3961 gtcttcgcct cggccgacgc gaagctcgcc gcggagaggg acgaggccgg ccgcacccgc
     4021 ctcctcgagg cgtacctcga gaacttcgag gcgcgcgtcg ccttctacga ggagcgctct
     4081 cgcgcgctct tcggccgcga gatcccgcag atccacctcg cgcacgcgaa cgccctgaac
     4141 gcccgggcga tgccgtacct cctcgagcgt ctcgagaggc gcggctacgc gttcgtcacg
     4201 ctcgacgagg cgctcgcgga ccccgcctgg aagagcccgg acgggtacgt cggcaagtgg
     4261 ggcccgtcct ggctccaccg cttcgcggcg gcccggggcg aggacgtgcg cgccctcttc
     4321 cgggccgagc ccgaggcccc gacgtgggtc gcccagctcg acgaggcgcg tcgggcggcg
     4381 gctcccgccg cgccctgagc gccgcgcggg cggatcagcc ccttctcttc ccgacgagcg
     4441 cgaagcccgc ctcgccgccg atctcgtccc agacggcgag cgagatccgc ggcgccggcg
     4501 cgtcgctctc gacctcgagc gtgtaggtga ggtgcgaggc gtcggccttc gcctcctcgc
     4561 cgcgcttcac ctcgaactcc tgccgccggc gcacgacctc ggagaagtcc cctttcggcg
     4621 tcgccgcgac gacgaagacc gagaacttcc ccttcgccgc ggaggggccc ggcacacgga
     4681 cgagcgcacc cagggggatg cggacctcga gcggaatccg gtacttcttc ccttccggct
     4741 tcgcctcgcc cgccgcgacg gagatgccga tgcgcgagct cgcgtcgggg gcgaaaaggt
     4801 tcgcgagggc ccggtcggtg agccgctcct cggccgtcct ctccgccacc gtcgtccggg
     4861 tccgcaccgc cagcccctcg cgcttcacgc ggaccctcac cgcttccggc ttcgtcgtcc
     4921 ccgaggccgg gaagccgatc gagtagtagg agccgaggtc gctcgcgacc tggtcgagga
     4981 agcgcggcag ctcgcccggg tggcccgccg tcgaaccgcc ggtctgcttc gtcacgaagt
     5041 ccaggccggc catctcgttc atccagatcg cgtcgaaccc ggagccgtag gcggcggacg
     5101 aggtcgacgg gttcagggcc gcggagtccg cggccgaggc gagcgccact ctctcgtaag
     5161 gatagatgcc gtagagcgtc acgccgctcg cgttcgcggc ggcggcgagc tcctcgagca
     5221 ggggcttccc gtcgcgttcc ctgggagacc cgacgggcgc gctgcgcgga ccgacgtggt
     5281 actcgcgccc cgggaaccgg ccgaaatggt gggccacgag gacgagcgac ttccgcccct
     5341 ccatccccgc catcagggcg acgaggcctc ggagagcggt caccttcgcc ttcatcatgc
     5401 cgtaggcctg aagcgtgcca atctcgcgcg aggtctccgt cgagccgacc gtcccgccga
     5461 cgcgcgggtc ggcggcgatc gagtcgaacc agaggtcgcg ggaggcgagc tcggcgacct
     5521 cgttctcctg gccgtcgagg cgggagacac ggcgggccac gacgtcgagg gtctcgtcca
     5581 gcgccgcgag gtcggcggtg aagctacgga cgacccgcac ggagtcgccc cacgagacga
     5641 ccatcgcctc gtcgcccggc cccgtcaggg ccttcgcaag gaacgccttc accccttcga
     5701 agagctcccg gcgacgggtc ggctcgggga gatggagccg gtcgacgaag acgaccacct
     5761 tccggggctg ccgggagacc gtctcttccg tcccgcgcgg gaccgcaccg gacgggtcga
     5821 tcccggccgg cgcgcccggc aggacgcccg tctcctcgct ccgctcctcg tgcaagttcg
     5881 tgatcaccac gggcttgccg ccgtaacgga gctcgaagtc ggcggcggtc aggccatgga
     5941 ccggcttacc attcctgtcc gtgacgacga cgtcgacgcc caggatcgag acctcggccg
     6001 aggcggcgat ccgcggggcg tccgaggcgg gctgggctgc ggtccgtcct gaagggagga
     6061 cgactgcggc gacggcaacg aatgtcgcga cggctgccgt cgccctcgcg atccgccccg
     6121 gacgtctcct gagcccattc gaggacgatt ccatgccgcg cccctccacg tcgtccacac
     6181 cttgcagata acatgccgcc cgccggcgga ggcgcgggac gcgcctcccg tccgggatgc
     6241 cgattcggag ggaccgtcga tgtacacgct cgtcctgctg cgccacggag agagccagtg
     6301 gaacctcgag aaccgcttca ccggctggac cgacgtcgac ctcaccgaga aagggcgcga
     6361 ggaggcccgc gaggcagggc gcctcctccg cgaagggggc tacgacttcg acgtcgccta
     6421 cacgtcggtc ctgaagcgcg cgatccggac gctctggatc gccctcgacg agatggaccg
     6481 tatgtggatc ccggtgatcc ggagctggcg cctgaacgag cggcactacg gggccctcca
     6541 ggggctcaac aaggccgaga cggccgcgaa gttcggcgag gagcaggtga aggtctggcg
     6601 ccggagctac tcgacgccgc cccccgagct gacccccgac gacgagcgct tccccggcca
     6661 cgaccgccgc tacgccgcgc tcgcgaagga ggagctgccg ctcaccgagt cgctgaagga
     6721 gaccgtcgcc cgcgccgtcc cgtactggga gagcgtcatc gcccccgagg tgaaggccgg
     6781 ccggcgggtc ctcgtcgcgg cccacggcaa cagcctccgc gccatggtca agtacctcga
     6841 cgggatctcg gacgaggaga tcgtcggcct caacatcccg acgggcgtcc cgctcgtcta
     6901 ccacctcgac gagaacctga agccgctgaa gagcttctac ctcggcgacg ccgaggccgt
     6961 cgccgcgaag gccgcagccg tggcgaacca ggggaagaag ggctgacgaa aacccggcgt
     7021 cagccgggaa agagacgggc gaccgggagg ccgagcgcgc cgaacggctc ggcggcgagc
     7081 tcggcgtccg cctcgaggac ggtgccggcg accatcgtgc cgcggacgtt cgcgaacgtc
     7141 tcgatccgcc gccggtcgac gtcgacgagc cagacgtagc cgacgcccat cgccccccag
     7201 gcgcccatct tctcgcgccg gtcgaagcgc tcggtcgtcg gcgagaggac ctcggccacc
     7261 cactccggcg cgagagtcgc cggcgtgatg ttcaggtccg gccaacccgt cgtcgagcgc
     7321 ctccaggcgg cgaggtcggg gacgaggcgc gagaggttct cctccgatcg gacctccggc
     7381 tcggcgacga agagccactc cggtgcgacg actccttcgc cccagccgaa gcggagccgg
     7441 agggcggcgc cgaggttgac ctgtgctcca ccgtgcgcgg cccgcggccg cggcgtcatc
     7501 atgtagatgc cccgagcgat ctccccctgc gagccctccg gctcgcgctc gagccgcttc
     7561 tccagctcga ggaagctccg gacgggatca ttgaccgggt tcatcggggc gagtctaccg
     7621 gcccgccggg cgccgggccg aagggcctcg ccgcccgccc cgccccctca gacgatccgc
     7681 tcgcggatcc gcgccgagac gaggtccatc acccagacgg cgacggcgat gaggaggacg
     7741 agcgtgccga cctcgcccca ctcgccgagg tcgacgcggg gcttgaggat gtagccgatg
     7801 ccgccgccgc cgacgaagcc gatgatcgtc gacatgcgga
//
LOCUS       DAOTEZ010000179         7807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_6595_length_7807_cov_6.500774, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000179 DAOTEZ010000000
VERSION     DAOTEZ010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7807
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6595_length_7807_cov_6.500774"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="P5164_10595"
     CDS             <1..893
                     /locus_tag="P5164_10595"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HRY44385.1"
                     /translation="DYDREYVHPIQVESLLMEGARMIDEWPIIQKVVRSPELVFQRVP
                     VGQRVEPAEELDDVEEIAEPAGSRARERRDGPIRVSRPEWAVYELVDGRRSVGDIVDR
                     TFLSEFEGSKAFFDLLSRGLVEEARRTGVVDETGVSSVEVPVVRPSRLVPSALGIILA
                     TLLGAAFLLQHRNALNAFTVPGRRVAAVAGFQKAISLSRLRKLSEAIDAYYLTQGRFP
                     DSPEAIVQAGLLGPEEILDPRGRAYRYILQGETGKYYLAGFGAEGKTDTDLFFAHAAV
                     GGMTGSESYSGGKKEIILIQ"
     gene            1006..2910
                     /gene="dnaK"
                     /locus_tag="P5164_10600"
     CDS             1006..2910
                     /gene="dnaK"
                     /locus_tag="P5164_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRY44386.1"
                     /translation="MSKIIGIDLGTTNSVVAVLEGGSADVIANSEGGRTTPSVVAVTK
                     SGERLVGQVAKRQAVTNPENTVYSIKRFMGRRFDEVNEEMKMVPYKVVRSANGDARVT
                     VGGKEMSPPEISAQILTKLKEAAEAHLGEKVERAVITVPAYFNDAQRQATKDAGQIAG
                     LKVERLVNEPTAAALAYGLDKKHNETIAVYDFGGGTFDISILEVGEGVVEVKSTNGDT
                     HLGGDNIDQRIVDWLLAEFRKDQGIDLGKDPMAVQRLKEAAEKAKIELSSTIESEINL
                     PFITADASGPKHLSLKLTRAKLEQMVDDLLQRSIGPCRQALKDAGLEAKQIDEVVLVG
                     GQTRMPKIQQIVKDFFGKEPHKGVNPDEVVAVGAALQGGVLAGDVKDLLLLDVTPLSL
                     GVETLGGVMTRLIERNTTIPVKKSEVFSTAADGQTSVEIKVLQGEREMAADNKLLGVF
                     SLVGIPPAPRGMPQIEVTFDIDANGILHVTAKDRGTGKEQKITITSSSGLAKDEVEKA
                     VKEAQAHGSEDKKRREAVEAKNQLDSLVYATEKLLAENADKVPAEDKTAIEAAVADAK
                     KVLETQADDAEALRKSAQEVQKASYKVAEALYKSAPAPEPAAPAGASTASSGKGADDV
                     IDAEVVDEKK"
     gene            2915..3337
                     /locus_tag="P5164_10605"
     CDS             2915..3337
                     /locus_tag="P5164_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005037830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRY44387.1"
                     /translation="MAGSRDGAGRGKTRRDDYVLIGVVAERFRVHPQTLRLYEREGLI
                     CPPRSSGNTRLYDRETVERLEMILTLTRDLGVNLAGVEVVLDLRARLSRMEGEVERLM
                     EAFRSVAAEHASRAAGRPNPPQNALAIRPRAPLARRTS"
     gene            3388..4275
                     /locus_tag="P5164_10610"
     CDS             3388..4275
                     /locus_tag="P5164_10610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HRY44388.1"
                     /translation="MNQRRADDDSGLLRRYLLEISKYPPLSAEREKELARVIQDRLVP
                     EERRKDAIDELVRANLRFVVYHAKGFHSANVPFIDLINEGNIGLIQAARRFDANRNVK
                     FITYAVWWVRQAISHALAEQAGTIRIPHKQATHQFRAVRARDELQRELGREPTHEEIA
                     ERTGLEPATVETLLSVTRNAESLSQGVGSDEQERTLEDVLEQTMVSSADDDMLRRASA
                     EQAREFLGELKPKERAVLCRRFGIPEDGSEGEREPMTLQEIGLELDLSRERVRQIEAQ
                     ALQRLRRGVKARRLLSVLR"
     gene            4308..5027
                     /locus_tag="P5164_10615"
     CDS             4308..5027
                     /locus_tag="P5164_10615"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY44389.1"
                     /translation="MDEPAVPVSCPVCRGFGRLERPDGSTVPCECRAEEISRVRRAAA
                     RIPERYRNCRLQNFKDLRNESLKKAKAVAKRWVEEWPAVDAGLLITGPVGVGKTHLAV
                     AILNELVDTKGASALFCDFSDLLDRIQASFGKGNDESQDDIVAPYRDADLLVLDELGA
                     RRPSDWVREILYGLLNTRYNRRRLTILTTNFADEPDARGGETLEVRVGAPVRSRLWEM
                     TQLVPINADDFRKAHGARAFG"
     gene            5032..7281
                     /locus_tag="P5164_10620"
     CDS             5032..7281
                     /locus_tag="P5164_10620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HRY44390.1"
                     /translation="MTRTSSIAAVLAAALLGAACTPRPAPVTALPPPAPSPTPSPTPA
                     PAPAATAAFPGVAEPRLDVGIAVDRAAFRLPPGEWLVRAGGEVRRVVGALEARPAAAA
                     GDAAPRFQVQAGSFASRVAADAEGSRLGVLLGLPASVAEAGGRFAVRLGPPGDRASLD
                     PLLNRVRRDAVPEAFLVGLADPGRPARTLLVDGPDGRREAPSPLELAAADGSLLPVNG
                     ALYRGTLLVVATPRGTLHVVNRVGLEDYLLGVVPLEMGPRVYDELEALKAQAVAARTY
                     AVKRRGDFAAEGYDLCATARCQVYGGASAEQPLSSEAVRATAGQVLVWNGAPADALFT
                     STCGGRTEDAVVVFPSYSREAFPYLRSVPCRGEERIAIASATPASGHDLGALAVRGAA
                     LLHAAGRAGASWADLKAARTLLTERLGLPAGAPPKSLAPDAVFANLAGLLGDARLLTE
                     EAERGAAPKEWSEAARTVLTALVRFQVAGPTPLPTRRPFTPHEAAGLWASLLLRTGGL
                     EELEGRLVGAPSGRLVVKTAKGREERALSADALLYRGGGEAWTPAGALDVAPGDRVRL
                     LTRDGKVLALLASVPAADGLFERESSWIHWVRRFTGAELMGKLLERDASRKGSVVRRV
                     DVLSRGASGRAARVRVTTDQEAFELSGLEIRFALALPESLFTLVTGADGRGAPVHTFY
                     GRGWGHGVGLCQTGTFGMALAGRTHAEILAAYYPGTSLGSWPPPSEPERSGSPEARPA
                     AGGTPPPAR"
     gene            7346..>7807
                     /locus_tag="P5164_10625"
     CDS             7346..>7807
                     /locus_tag="P5164_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRY44391.1"
                     /translation="MTTLLDSPAAVGAAPSVPFGFQLSDDQLAVRKMVREFAESELAP
                     HVMEWDEAQAFPREAIARLGELGMLGVIVPEEYGGAGLSYIDYIGVIEELSRVDGSVG
                     ISIAAHNSLCTNHLLLFGTEDQKRRWVVPLAQGRMIGAWGLTEAEAGSDAKG"
BASE COUNT         1186 a         2759 c         2817 g         1045 t
ORIGIN      
        1 tcgactacga ccgggagtac gtccacccga tccaggtcga gagcctcctg atggaaggtg
       61 cccggatgat cgacgagtgg ccgatcatcc agaaggtcgt ccgttcgccc gagctcgtct
      121 tccagcgcgt cccggtgggg cagcgggtgg agccggcgga agagctcgac gacgtcgagg
      181 agatcgcgga gccggcgggc tcccgggcgc gggagaggcg ggacggcccg atccgcgtct
      241 cacggcccga gtgggcggtc tacgagctcg tcgacggacg ccgttccgtc ggggacatcg
      301 tcgaccggac gttcctctcg gagttcgaag ggtcgaaggc ctttttcgac ctcctcagcc
      361 gggggctggt cgaggaggcg cggcggacgg gggtcgtcga cgagacgggc gtctcgtcgg
      421 tcgaggtccc ggtcgtccgg ccgagccgcc tcgttccgtc cgcgcttggc atcatcctgg
      481 ccaccctcct cggagccgcg ttcctgttgc agcaccggaa cgcgctgaac gcgttcacgg
      541 tccccggacg ccgtgtcgcg gcggtcgcgg ggttccagaa ggcgatctcg ctctcccggc
      601 tgcgcaagct ctcggaggcg atcgacgcgt actacctgac gcaggggcgc tttccggact
      661 cgccggaggc gatcgtccag gcggggctgc tggggccgga agagatcctc gatccccggg
      721 gacgcgcgta ccggtacatc ctgcagggcg aaaccgggaa gtactacctc gcgggattcg
      781 gcgcggaggg aaagaccgat accgatctct tcttcgccca cgcggccgtc ggcggaatga
      841 ccggttccga gagctattcg ggcggcaaga aggaaataat tctcatacag tgacttgcgc
      901 gatttcttcg gcgctgctga agatttcttt cgcaatgaac ttgacagtat cgcgctaata
      961 tcctagattc ccgcttgggc ccgcagcggt ccggaggaaa tcaggatgag caagatcatc
     1021 ggcatcgacc tcggaacgac gaacagcgtc gtggccgtcc tcgagggggg ctccgcggac
     1081 gtgatcgcga actccgaagg gggccggacc actccgtccg tcgtcgccgt taccaagtcg
     1141 ggcgagcgcc tcgtcgggca ggtcgccaag cggcaggccg tcacgaaccc ggagaacacc
     1201 gtctactcga tcaagcggtt catggggcgc cgcttcgacg aggtcaacga agagatgaag
     1261 atggtcccct acaaggtcgt ccggtcggcc aatggggacg cccgcgtgac cgtcggcggc
     1321 aaggagatgt cgccgccgga gatctcggca cagatcctga cgaagctgaa ggaggccgcg
     1381 gaagcccacc tcggcgagaa ggtggagcgg gcggtcatca ccgtaccggc ttacttcaac
     1441 gacgcccagc gccaggcgac gaaggacgcc gggcagatcg ccggcctcaa ggtcgagcgc
     1501 ctcgtcaacg agccgacggc cgcggcgctc gcctacggcc tcgacaagaa gcacaacgag
     1561 acgatcgccg tctacgactt cggcggcggc acgttcgaca tctcgatcct ggaggtcggc
     1621 gagggggtcg tcgaggtcaa gtcgacgaac ggcgacacgc acctcggcgg cgacaacatc
     1681 gaccagcgaa tcgtcgactg gctcctcgcc gagttccgga aggaccaggg gatcgacctc
     1741 ggcaaggacc cgatggcggt gcagcgcctg aaggaggccg ccgagaaggc gaagatcgag
     1801 ctctcgtcga cgatcgagtc ggagatcaac cttccgttca tcacggccga cgcctccggg
     1861 ccgaagcacc tgagcctcaa gctgacgcgc gcgaagctcg agcagatggt cgacgacctt
     1921 ctccagcgtt ccatcggccc ctgccgccag gcgctgaagg acgccggcct cgaggcgaag
     1981 cagatcgacg aggtcgtcct cgtcggcggc cagacccgca tgccgaagat ccagcagatc
     2041 gtgaaggact tcttcggcaa ggagccccac aagggggtca accccgacga ggtcgtcgcg
     2101 gtcggcgcgg cgctccaggg aggcgtcctc gcgggcgacg tgaaggacct cctcctcctc
     2161 gacgtgacgc cgctctccct cggcgtcgag acgctcggcg gcgtcatgac gcgcctcatc
     2221 gagcggaaca cgacgatccc ggtgaagaag agcgaggtct tctcgacggc ggccgacggg
     2281 cagacgtccg tcgagatcaa ggtcctccag ggcgagcgcg agatggccgc cgacaacaag
     2341 ctcctcggcg tcttctccct cgtcgggatc ccgcccgcgc cgcggggcat gccgcagatc
     2401 gaggtgacgt tcgacatcga cgcgaacggc atcctccacg tcaccgcgaa ggaccgcggc
     2461 accggcaagg agcagaagat cacgatcacc tcctcctcgg gcctcgcgaa ggacgaggtc
     2521 gagaaggccg tgaaggaggc ccaggcgcac gggagcgagg acaagaagcg gcgcgaggcc
     2581 gtcgaggcga agaaccagct cgactcgctc gtctacgcga ccgagaagct gctcgccgag
     2641 aacgccgaca aggtcccggc ggaggacaag accgccatcg aagcggccgt cgcggacgcg
     2701 aagaaggtcc tcgagacgca ggccgacgac gccgaagcgc tccggaaatc ggctcaggag
     2761 gtccagaagg cgagctacaa ggtggccgag gccctctaca agagcgcccc ggccccggaa
     2821 ccggccgccc cggccggcgc gtcgaccgcg agctccggga agggcgcgga cgacgtgatc
     2881 gacgccgagg tcgtcgacga gaagaagtga cgccgtggcg ggctcgcgag acggggccgg
     2941 ccgcgggaag acgcgccgcg acgactacgt cctgatcggc gtcgtcgcgg agcgctttcg
     3001 ggtccatccc cagacgctgc gcctctacga gcgggagggg ctcatctgcc ccccccgctc
     3061 gtcggggaat acgcgcctct acgaccgcga gaccgtggag cggctggaga tgatcctgac
     3121 gctgacgcgc gacctcggcg tgaacctggc gggcgtggag gtcgtcctgg accttcgcgc
     3181 ccgcctctcc cggatggaag gcgaggtcga gcgcctcatg gaagcgttcc gctcggtcgc
     3241 ggccgagcac gcctcgcgcg ccgcggggcg accgaacccg ccccagaacg cgctcgcgat
     3301 ccgcccgcgg gcgccgctgg ctcgccgcac gtcctgacgc ccggagccct cccttccgga
     3361 gggacggcgg gatacgatgt gcgcaccttg aaccaaaggc gggccgacga cgactcgggg
     3421 cttctgcgga ggtatctcct ggagatctcg aagtaccctc ccctctccgc cgagcgtgag
     3481 aaggagctgg cgcgcgtcat ccaggaccgg ctcgtgcccg aggagcgccg gaaggacgcg
     3541 atcgacgagc tcgtccgggc gaacctccgc ttcgtcgtct accacgccaa ggggttccac
     3601 tcggcgaacg tccccttcat cgacctgatc aacgaaggga acatcggcct gatccaggcg
     3661 gcccgccgct tcgacgcgaa caggaacgtc aagttcatca cctacgccgt ctggtgggtc
     3721 cgccaggcga tctcccacgc gctcgccgag caggccggga cgatccggat cccccacaag
     3781 caggcgaccc accagttccg ggccgtgcgg gcccgggacg agctgcagcg ggagctcggc
     3841 cgggagccga cgcacgagga gatcgcggag cggacgggcc tcgagcccgc cacggtcgag
     3901 acgctcctgt cggtgacgcg gaacgcggaa tcgctctccc agggggtcgg cagcgacgag
     3961 caggagcgga cgctcgagga cgtcctcgag cagacgatgg tctcctccgc cgacgacgac
     4021 atgctgcgcc gggcgtcggc ggagcaggcg cgcgagttcc tcggcgagct gaagccgaag
     4081 gagagggcgg tcctctgccg gcgcttcggg ataccggagg acgggagcga gggagagcgc
     4141 gagccgatga ctctccagga gatcgggctc gagctcgacc tctctcgcga gagggtccgg
     4201 cagatcgagg cgcaggccct tcagcgcctg cgccgcggcg tgaaggcgcg ccggctcctt
     4261 tccgtcctcc gctgacgccg ggagggaggc cccctacaat cgtcgccgtg gacgagcccg
     4321 ccgttcccgt ctcttgtccc gtctgccgcg gtttcggaag gctcgagcgc ccggacggct
     4381 cgaccgtacc gtgcgagtgc cgggcggagg agatctcgag ggtgcgccgc gcggcggccc
     4441 ggatcccgga acgctaccgg aactgccgcc ttcagaactt caaggacctc cgcaacgagt
     4501 cgctcaagaa ggcgaaggcg gtcgcgaaac gctgggtcga ggagtggccg gccgtcgacg
     4561 ccgggctcct catcacgggc ccggtcggcg tcggcaagac gcacctggcc gtcgcgatcc
     4621 tgaacgagct ggtcgacacc aagggggcct cggccctctt ctgcgacttc tcggacctcc
     4681 tcgaccggat ccaggcgagc ttcggaaagg ggaacgacga gagccaggac gacatcgtcg
     4741 cgccgtaccg ggacgcggac ctcctcgtcc tcgacgagct cggggcgagg cggccgtccg
     4801 actgggtccg cgagatcctc tacggcctcc tgaacacgcg ctacaaccga aggcggctca
     4861 cgatcctgac gacgaacttc gccgacgagc cggacgcgcg cggcggcgag acgctcgagg
     4921 tgcgcgtcgg cgcccccgtc cggagccgcc tctgggagat gacgcagctc gtgccgatca
     4981 acgccgacga cttccggaag gcgcacggcg cccgagcgtt cggctgagcc gatgacgcgg
     5041 acgtcctcga tcgcggccgt cctcgcggcg gctcttctcg gcgccgcctg cacgccccgg
     5101 cccgccccgg tgacggccct cccgccgccg gcgccctcgc cgacgccgag tccgactccg
     5161 gcgccggcac cggcggcgac ggccgccttc ccgggagtcg ccgagccgag gctcgacgtc
     5221 gggatcgccg tcgaccgcgc tgcgttccgc ctccccccgg gggagtggct ggtccgggcc
     5281 ggcggcgagg tccgccgcgt cgtcggcgcg ctcgaggcgc ggcccgcggc cgcggcgggc
     5341 gacgcggctc cgcggttcca ggtccaggcg gggtccttcg cttcgcgcgt ggccgcggac
     5401 gccgagggat cgcgcctcgg cgtcctgctc ggcctgccgg cttccgtcgc ggaagcggga
     5461 gggcggttcg ccgtgcggct cggaccgccg ggcgaccgcg cctcgctcga cccgctcctc
     5521 aaccgggtcc gtcgcgacgc cgtaccggag gcgttcctcg tcggcctcgc cgatccgggg
     5581 cggccggccc ggacgctcct cgtcgacggt ccggacggcc ggcgcgaggc gccctcgccg
     5641 ctcgagctcg cggccgcgga cggctccctc ctgccggtga acggagccct ctaccgggga
     5701 acgcttctcg tcgtcgcgac gccccgcggg acgctgcacg tcgtcaaccg ggtcggcctc
     5761 gaggactacc tcctcggagt cgtcccgctc gagatggggc ctcgcgtcta cgacgagctc
     5821 gaggcgctga aggcgcaggc cgtggcggcg cgcacctacg ccgtaaagcg gcggggcgac
     5881 ttcgcagccg aggggtacga cctctgcgcg accgcgcgct gccaggtcta tggaggcgcg
     5941 agcgccgagc agccgctctc gagcgaagcg gtccgggcga ccgccgggca ggtcctcgtc
     6001 tggaacggcg ccccggccga cgccctcttc acctccacct gcggagggcg tacggaggac
     6061 gccgtcgtcg tcttcccgtc ctattcgcgg gaggcgttcc cgtacctgcg ctccgtgccg
     6121 tgccgcggcg aggagaggat cgcgatcgcc tcggcgacac cggcctcggg ccacgacctc
     6181 ggcgcgctcg ccgttcgcgg cgcggcgctc ctccatgccg cggggcgggc gggggcctcc
     6241 tgggccgacc tgaaggcggc acgcaccctc ctgacggagc gcctgggcct gccggccgga
     6301 gcgccgccga agtcgctcgc gccggacgcc gtcttcgcga acctcgcggg gctcctcggc
     6361 gacgcgcggc tcctcacgga agaggccgag aggggcgcgg ccccgaagga gtggagcgag
     6421 gcggcgcgga cggtcctcac cgcgctcgtc cgcttccagg tcgcgggccc gacgccgctc
     6481 ccgacccggc ggcccttcac gccgcacgag gcggccggcc tctgggcctc cctcctcctg
     6541 cggacgggag ggctcgagga gctggagggg cggctcgtcg gggcgccctc gggaaggctc
     6601 gtcgtgaaga cggcgaaggg gagggaggag cgagctctct ctgccgacgc cctcctgtac
     6661 cgcgggggcg gggaggcgtg gacgccggcc ggggcgctcg acgtcgcgcc cggggaccgg
     6721 gtgcggctcc tgacgcgcga cgggaaggtg ctcgccctgc tggcgtccgt cccggcggcg
     6781 gacggcctgt tcgagcgcga gtcgtcctgg atacactggg tccgccgctt cacgggcgca
     6841 gagctgatgg ggaagctcct cgagcgcgac gcctctcgga aggggagcgt cgtgcgacgc
     6901 gtcgacgtcc tctctcgcgg cgcctcggga cgcgcggcgc gagtgcgcgt gacgacggac
     6961 caggaggcgt tcgagctctc cggtctcgag atccgcttcg ccctcgcgct ccccgagtcg
     7021 ctcttcacgc tcgtcacggg ggcggacggc cggggagcgc ccgtccacac gttctacggc
     7081 cgcggctggg gccacggcgt cggcctctgc cagaccggga cgttcgggat ggccctcgcg
     7141 ggccggacgc acgccgagat cctcgcggcc tactaccccg ggacctcgct cggctcctgg
     7201 cctccgccga gcgagccgga gcgctccggc tcgccggagg cgcgtcccgc cgcgggcggt
     7261 acgcccccgc cggcgcgctg acccggccct cccggagccg cggctagaat cgggagatcg
     7321 ggcatctcgc cccaggagtc cggcgatgac gactctcctc gacagccctg cggccgtcgg
     7381 tgccgcccct tccgtcccgt tcggcttcca gctgtccgac gaccagctcg ccgtccggaa
     7441 gatggtccgc gagttcgccg agagcgagct ggccccgcac gtcatggagt gggacgaagc
     7501 gcaggctttc ccgcgcgagg cgatcgcccg cctcggcgag ctcgggatgc tcggcgtgat
     7561 cgtccccgag gagtacggcg gggcggggct ctcgtacatc gactacatcg gcgtcatcga
     7621 ggagctctcg cgcgtcgacg gctccgtcgg gatctcgatc gcggcgcaca acagcctctg
     7681 cacgaaccac ctcctcctgt tcgggacgga ggatcagaag cggcgctggg tcgtcccgct
     7741 cgcgcaggga aggatgatcg gcgcctgggg cctcacggag gccgaggcgg ggagcgacgc
     7801 gaagggg
//
LOCUS       DAOTEZ010000180         7777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_6632_length_7777_cov_4.298109, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000180 DAOTEZ010000000
VERSION     DAOTEZ010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7777
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6632_length_7777_cov_4.298109"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /gene="ribA"
                     /locus_tag="P5164_10630"
     CDS             <1..601
                     /gene="ribA"
                     /locus_tag="P5164_10630"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HRY44392.1"
                     /translation="HERLVQRVASPILPTRHGEFRVHAYRSDLTNEEHLALVMGDVAE
                     EEPILVRVHSACLTGDVLGSMRCDCGEQLNKALEMIAREGRGILLYLLQEGRGIGLLN
                     KLRAYDLQDGGLDTVAANEALGFAPDVRDYGIGCQILRDLGVKKMRLLTNNPSKYVAL
                     DGFGLEIVERVSLEIPPTDATRGYLSVKKAKMGHLLKLV"
     gene            655..1245
                     /locus_tag="P5164_10635"
     CDS             655..1245
                     /locus_tag="P5164_10635"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HRY44393.1"
                     /translation="MAYGFVLVGGRSSRMGRDKALLPVGGVPMALLQARKLEGLCAKV
                     CFVGKEPGELGALGHPFVRDGTEERAALHGLVAALAASPEEECLVLAADLPLVPAELL
                     EALRARREEAGAPAVVPWDGGGPQPLCSAWSTRALPALRAAVATGDLCLRRALAAAGA
                     IVLAAAETERLPGFRPGVFRNVNTLEEYRALEEELA"
     gene            1220..1744
                     /gene="moaC"
                     /locus_tag="P5164_10640"
     CDS             1220..1744
                     /gene="moaC"
                     /locus_tag="P5164_10640"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011094349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HRY44394.1"
                     /translation="MPSKKSSPELTHLDAEGAARMVDVSGKPESIRSAKARAVVTLGA
                     AAWSALDASENRKGDALAVARIAGIQAAKRASDWIPLCHPLVFDAVDVRLERRRSRRQ
                     VEVLATIRGSGRTGYEMEAMVAAAAAALTVYDMCKAAEKGIVVGPIELVEKSGGKSGD
                     WRRAPARSGSRRRA"
     gene            complement(1721..2770)
                     /locus_tag="P5164_10645"
     CDS             complement(1721..2770)
                     /locus_tag="P5164_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44395.1"
                     /translation="MRRRALLAAAFLLLAAGFASATLFALRQPLSLGDYLAIWGLKAR
                     ALHASGDLAALFSVDPAGEFSHPEYPPLWPILLASAAKLLGRYDELLLALLRPALLTL
                     AAALTYARTRASLPVRLLAASALVLLPYFQTAAYVGYAETLLLVLVLAALLLLESAHP
                     TPFTPWAAGLLLALAALTKQEGTIVIVVAAALLFYSGRRRDAAIAGGVGLLAGVIPWT
                     LYRAAHDVSSLADFAPGAFEIGKPFRAVAAFGEVALLTALPWLAGAAIVLALAPATLR
                     ARRALLAGTVAYVALLLAAFAFARVDVGWLVTWTWDRLVLLPVAALLPALAEAAAEPF
                     REASPAEVSGAAPRP"
     gene            complement(2767..3051)
                     /locus_tag="P5164_10650"
     CDS             complement(2767..3051)
                     /locus_tag="P5164_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44396.1"
                     /translation="MAKRFWGAGPRGRLLNAPGIARDAGFSATALRAAEAWPARFDAR
                     LSVGPLVPADVRERLRRKAAYLLAPRRVVLVEGDGAEVSLTPAPAGSAAR"
     gene            3250..3765
                     /locus_tag="P5164_10655"
     CDS             3250..3765
                     /locus_tag="P5164_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HRY44397.1"
                     /translation="MTAPLRVAVLTVSDRSARGEREDRGGPAVAEAVRRLLGAEVVET
                     QVVPDEKDDIVWHLTRFADNVLADLVLTTGGTGISDRDVTPEATRSVISLELPGLPEA
                     MRASSRAALPSAMLSRQVAGIRGKTLVVNLPGSPRGAVECLEAIAAALPHAVAMLRGE
                     GAEAHPASLRA"
     gene            3789..4736
                     /locus_tag="P5164_10660"
     CDS             3789..4736
                     /locus_tag="P5164_10660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HRY44398.1"
                     /translation="MKLARSAALCALLLLAAAAALRPARAASRIPVDTTGETTRRVLR
                     LATGAVETVPLEAYVAAVLPDEIGGNAPAAALEAQAVAARSYAVAQKGRHADRGADVC
                     DSVHCQVFRGLERATDATRRAAEATRGLVLVQGGHVVAAPFHAVCGGRTARPSDIWDD
                     EETPDLLPVEDDACLASPGARWTFFLPRAKVPLLAERLGLPTARFLEVWSHAGDGRVA
                     ALRLVAPGGHARVVRAFDVRARASELWGWSSIRSTDFDVEERPAGYAFTGRGTGHGAG
                     LCQAGAIARARRGESRGAILAHYYRGAAVATLGAAGAAE"
     gene            4733..6436
                     /gene="mobB"
                     /locus_tag="P5164_10665"
     CDS             4733..6436
                     /gene="mobB"
                     /locus_tag="P5164_10665"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HRY44399.1"
                     /translation="MTRPRLLSGLDVLLADTSPLRGLKLGLVSNPASVTSRFVPAARA
                     LLDAGLNLKLLFGPEHGLTGAVQDMLAVGDRDTPRGRLPVVSLYGERFEDLSPRPEHL
                     ADLDAVVCDLPDVGSRYYTFVWTTFLVLKACAAAEKPVFVLDRPNPLGGFAVEGNLPE
                     ERLLSFVGLRPVPARHGMTPGELARWANAESALGCDLTVVPMRVEGRPGAAPRGRVGE
                     TPAWVLPSPNMPTPDTALVYPGMCLLEGANLSEARGTTRPFETVGAPWLDAESAADRA
                     NALGLPGVLFRPHVFRPTFHKFAGQDCGGVQLHVTDRDAFRPYETGLRLVKLLRDLEP
                     SRFRWRTETYEFRSDVPAVDLLAGTPRYRELVDAGESLDAWIATFPDDVARFAPTRES
                     ALLYRPGPPRVHVVGAQDSGKTAHALRLVEALVARGFSVGALKHTGHEHETDVAGSDS
                     QRYAASGASPVAFVSGRRTGVHARHAEAPSLDTVVGREMPDVDVVVVEGFREEAGPKV
                     EVCRSATGRDAVAAGDPEVLAVMTDRETSHPSSVPRLPLGDVDRLVPLVLDALGLGGP
                     R"
     gene            6433..7389
                     /locus_tag="P5164_10670"
     CDS             6433..7389
                     /locus_tag="P5164_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HRY44400.1"
                     /translation="MKAILVPSPGGPEALVLGETPEPVLREGEVFVRVRATAVNRADL
                     LQAAGKYPPPPGESEILGLEAAGVVEETGERVFCLLPGGGYAERVAVPRAMLMPIPDR
                     LSFEEAAAVPEAWFTAYLNLFREGALAAGEVVLLHAAASGVGTAAIPLAKDAGARVVA
                     TVRSAAKAGALESLGADLVVDTTARDFAEAIEERFGKASVGLVLDPVGGAALAGNVRL
                     LARGGRLVLIATMGGGAAELDLRAVLTKRLRIVGSTLRARPLAEKADLTAAFVRDVLP
                     GFADGRFRPLVDSVFPIERAADAHRRMASNANVGKIVLTVAS"
     gene            7386..>7777
                     /locus_tag="P5164_10675"
     CDS             7386..>7777
                     /locus_tag="P5164_10675"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRY44401.1"
                     /translation="MTYRAAVFDLDGTLLDSYVGIHDALSTVLEHFARPAVTVEEARR
                     LVGHGLEALIAKVLPEELRAEGAERFRFRYKVNAPKLTRLMPDAELVVSELWRRGVRL
                     SIASNKPAVFSRQLLAGFGLEELFAFVGG"
BASE COUNT          986 a         2872 c         2907 g         1012 t
ORIGIN      
        1 ccacgagcgg ctcgtgcagc gcgtcgcctc gccgatcctc ccgacgaggc acggggagtt
       61 ccgcgtccac gcctaccggt cggacctgac gaacgaggag cacctcgcgc tcgtgatggg
      121 cgacgtcgcc gaggaggagc cgatcctcgt cagggtccac tcggcgtgcc tgaccggcga
      181 cgtcctcggc tcgatgcgct gcgactgcgg cgagcagctg aacaaggcgc tcgagatgat
      241 cgcccgggag gggcggggga tcctgctcta ccttctccag gagggccggg ggatcggcct
      301 cctcaacaag ctccgcgcct acgacctcca ggacggcggc ctcgacaccg tcgccgcgaa
      361 cgaggcgctc ggcttcgccc ccgacgtgcg cgactacggc atcggctgcc agatcctccg
      421 ggacctcggc gtcaagaaga tgcgcctcct gacgaacaac ccgtccaagt acgtcgccct
      481 cgacggattc ggcctcgaga tcgtcgagcg ggtctccctg gagatcccgc ccacggacgc
      541 gacgcgcggc tacctctccg tcaagaaggc gaagatgggg cacctgctga agctggtgta
      601 gccgcgcttc cggcgcgcgt ccgggccgcg cccggcgggg tacgatgccc gcgcgtggca
      661 tacgggttcg tcctcgtcgg cgggcgttcg agccgcatgg ggcgcgacaa ggcgctcctt
      721 ccggtcggcg gcgtcccgat ggccctcctc caggcgcgga agctggaggg gctttgtgcg
      781 aaggtctgct tcgtcgggaa ggagccggga gagctcggcg cgctcggtca tcccttcgtc
      841 agggacggga cggaggagcg ggcggcgctc cacggcctcg tcgccgcgct cgccgcctcg
      901 ccggaagagg agtgcctcgt cctcgcggcc gacctcccgc tcgtcccggc ggagctcctc
      961 gaggcgctcc gcgcgcggcg cgaggaggct ggcgcgccgg ccgtcgtccc gtgggacgga
     1021 gggggcccgc agcccctctg ctccgcctgg tcgacgcgcg cgctcccggc gctccgtgcg
     1081 gcggtcgcca ccggagacct ctgcctccgg cgcgccctgg cggcggccgg cgcgatcgtc
     1141 ctggcggcgg cggagaccga gcgcctgccc ggcttccgtc ccggcgtctt ccgaaacgtg
     1201 aacaccctgg aggaataccg tgccctcgaa gaagagctcg cctgagctga cgcacctcga
     1261 cgccgagggt gctgctcgta tggtcgacgt ctccggcaag ccggagtcga tccggagcgc
     1321 gaaggcccgc gcggtcgtca cgctcggggc cgcggcctgg tccgccctcg acgcctcgga
     1381 gaaccggaag ggggacgccc tcgccgtcgc gcggatcgcc ggcatccagg cggcgaaaag
     1441 ggcctcggac tggatcccgc tctgtcaccc gctcgtcttc gacgccgtcg acgtccgcct
     1501 cgagcggcgc cggagccggc ggcaggtgga ggtcctcgcc acgatccggg gctcggggcg
     1561 gacgggttac gagatggagg cgatggtcgc ggcggccgcg gcggccctga cggtctacga
     1621 catgtgcaag gctgcggaga aggggatcgt cgtcgggccg atcgagctcg tcgagaagag
     1681 cggcggcaag agcggagact ggcggcgggc gccggcccgg tcagggtcgc ggcgccgcgc
     1741 ctgagacctc ggccggcgag gcttcccgga acggctccgc cgcggcctcg gcgagagcgg
     1801 ggaggagagc ggcgacggga aggagcacga gccggtccca ggtccaggtg acgagccatc
     1861 cgacgtcgac ccgcgcgaac gcgaaggccg cgaggaggag ggcgacgtag gcgacggtcc
     1921 ccgcgaggag ggcgcggcgc gcacggagag tcgccggcgc gagggcgagg acgatcgcgg
     1981 cccctgcaag ccaggggagc gcggtcagga gggcgacctc cccgaacgcg gcgacggccc
     2041 ggaacggctt cccgatctcg aacgcgccgg gagcgaagtc cgcgagggag gagacgtcgt
     2101 gtgcggcgcg gtagagagtc cacgggatca cgccggcgag caggccgacg ccgcccgcga
     2161 tggccgcgtc gcgtcgccgt ccggagtaga agaggagggc ggcggctacg acgattacga
     2221 tcgtcccctc ctgcttcgtc agggcggcga gcgcgagaag gagccccgcg gcccaggggg
     2281 tgaacggcgt cgggtgcgcg ctctcgagga ggaggagcgc ggccaggacg aggacgagga
     2341 gaagcgtctc ggcgtaaccg acgtacgccg ccgtctggaa gtaggggagg aggacgagcg
     2401 cggacgccgc gaggagtcgt acggggaggg acgcccgcgt cctcgcgtag gtgagcgccg
     2461 ccgcgagcgt gaggagagcc ggccgaagga gcgcgaggag gagctcgtcg tagcggccga
     2521 ggagcttcgc ggcgcttgcg aggaggatcg gccagagcgg cgggtactcg gggtgggaga
     2581 actcgcccgc cgggtcgacg ctgaagaggg ccgcgaggtc gccggaggcg tggagcgcgc
     2641 gcgccttcag cccccagatc gcgaggtagt cgccgagcga gaggggttgc cgaagggcga
     2701 agagcgtggc ggaagcgaag ccggcggcga ggagcaggaa cgccgctgcc aagagggcac
     2761 ggcgcctcat cgcgcggcgc tccccgccgg ggccggcgtc agggagactt cggcgccgtc
     2821 cccctcgacg aggacgaccc ggcgcggggc gaggaggtag gcggccttcc gacggagccg
     2881 ttcccggacg tcggccggga cgagcggacc gacggaaagc cgagcgtcga agcgcgcggg
     2941 ccatgcctcg gccgcgcgga gggccgtcgc gctgaagccg gcgtcgcgcg cgatgcccgg
     3001 agcgttcagg agccgtccgc ggggcccggc tccccagaag cgcttcgcca tccggacgga
     3061 gtctgcgtac gggaggtcgt gccggagcgc tccggcgagc agcgcggcga gccccgctcc
     3121 ggcgaggagg gcgacgagca gccggcgggc gcgaagaggg agcagacggg cctccgtgcg
     3181 gattgtagag accgcctaga atcccgccat gtcgaagacg cccgccgcca tcgaacgcgg
     3241 agcccggccg tgaccgcccc cctgcgtgtc gccgtcctga ccgtctccga ccgctcggcc
     3301 cgaggcgagc gggaggaccg gggcggaccg gcggtcgccg aggcggtccg gcggctcctc
     3361 ggcgccgagg tcgtcgagac gcaggtcgtc ccggacgaga aggacgacat cgtctggcac
     3421 ctgacccgct tcgcggacaa cgtcctcgcc gatctcgtcc tgacgacggg agggacgggg
     3481 atctccgatc gagacgtgac gcccgaggcg acccgttccg tcatctcgct cgagctgccg
     3541 ggcctgccgg aggcgatgcg cgcctcgagc cgtgcggcgc tcccttcggc gatgctctcg
     3601 cgccaggttg ccgggatccg cgggaagacg ctcgtcgtga acctcccggg ctcgccgcgc
     3661 ggggcggtgg agtgcctcga ggcgatcgcc gcggcgctgc cgcacgccgt cgcgatgctc
     3721 cgcggagagg gcgccgaggc gcacccggcc tccctccgcg cctgagcggc cgccggggat
     3781 tccgccgcgt gaagctcgcc cgctccgccg cgctctgtgc gctcctcctc ctcgctgctg
     3841 ccgcggccct tcgcccggcc cgggccgcct cccgtattcc cgtcgacacg acgggggaga
     3901 cgacgcggcg cgtcctgcgc ctcgccacgg gcgccgtcga gacggtcccg ctcgaggcgt
     3961 acgtggcggc cgtcctcccg gacgagatcg gcggaaatgc gcctgcggcc gcgctcgagg
     4021 cgcaggccgt cgccgcgcgc tcgtatgcgg tcgcgcagaa gggacgtcac gccgaccggg
     4081 gcgccgacgt ctgtgattcg gtccactgcc aggtctttcg cggcctcgag cgcgccacgg
     4141 acgcgacgcg gcgcgccgcg gaggcgacgc gcgggctggt cctcgtccag ggcggacacg
     4201 tcgtcgccgc tccgttccac gccgtctgcg gcggccggac cgcccgaccc tccgacatct
     4261 gggacgacga ggagacgccc gacctcctgc cggtcgagga cgacgcctgc ctcgcgtcgc
     4321 cgggcgcccg ctggacgttc ttcctcccgc gcgcgaaggt gccgctcctc gcggagaggc
     4381 tcgggcttcc cacggcccgc ttcctggagg tctggtccca cgccggcgac ggccgcgtcg
     4441 ccgccctgcg cctggtcgcg cccggcggac acgcgcgtgt cgtccgcgcc ttcgacgtgc
     4501 gggcgcgcgc ctcggagctc tggggctgga gctcgatccg ctccaccgat ttcgacgtcg
     4561 aggagcgccc cgcgggctac gccttcaccg gtcgcgggac ggggcacggc gcgggtctct
     4621 gccaggcggg cgcgatcgcg cgggcccgcc gcggcgagag tcgcggcgcg atcctcgcgc
     4681 attactatcg gggcgccgcc gtcgcgacgc tcggcgcggc gggagcagcc gaatgacgag
     4741 accccgcctc ctctccggcc tcgacgtcct cctcgcggac acctccccgc tccggggcct
     4801 gaagctcggc ctcgtctcga acccggcctc cgtgacgtcc cgcttcgtcc cggcggcgcg
     4861 cgccctcctc gatgccggcc tcaacctgaa gctcctcttc ggaccggagc acgggctgac
     4921 gggcgccgtc caggacatgc tcgccgtcgg cgaccgcgac acgccgaggg ggcggctccc
     4981 cgtcgtctcg ctctacggcg agcggttcga ggacctctcg cctcggccgg agcacctcgc
     5041 ggacctcgac gccgtcgtct gcgacctccc cgacgtcggc tcgcgctact acacgttcgt
     5101 ctggacgacg ttcctcgtcc tgaaggcgtg cgccgcggcg gagaagcccg tcttcgtcct
     5161 cgatcgcccg aacccgctcg gcggcttcgc cgtcgagggg aacctcccgg aggagcggct
     5221 cctctcgttc gtcggcttgc ggcccgtgcc ggcgcgccac gggatgacgc cgggggagct
     5281 cgcccgctgg gcgaacgccg agagcgcgct cggctgcgat ctcaccgtcg tgccgatgcg
     5341 cgtcgaaggg cggccgggag ctgccccgcg cggccgcgtc ggcgagacgc cggcctgggt
     5401 cctcccctcg ccgaacatgc cgactcccga caccgcgctc gtctatccgg ggatgtgcct
     5461 tctcgagggg gcgaacctct cggaggcgcg agggacgacc cgcccgttcg agacggtcgg
     5521 agcgccctgg ctcgacgcgg agtccgcggc cgaccgcgcg aacgctctcg gcctccccgg
     5581 cgtcctcttc cgcccgcacg tcttccggcc gacgttccac aagttcgccg ggcaggactg
     5641 cggcggcgtc cagctccacg tcacggaccg cgacgcgttc cggccgtacg agacgggcct
     5701 tcgactcgta aagctcttgc gcgacctcga gccgtcgcgc ttccgctgga ggaccgagac
     5761 gtacgagttc cggagcgacg tccccgccgt cgacctcctc gcggggacgc cgcgctaccg
     5821 cgagctcgtc gacgccgggg agagcctcga tgcgtggatc gcgacgttcc cggacgacgt
     5881 cgcccgtttc gctcccacgc gcgagtcggc gctcctttac aggccggggc cgccgcgggt
     5941 ccacgtcgtc ggcgcgcagg actcgggaaa gacggcgcac gccctccgcc tcgtcgaggc
     6001 gctcgtggcg cgcggcttct ccgtcggcgc cctgaagcac accggccacg agcatgagac
     6061 cgacgtcgcg ggaagcgact cgcagaggta cgccgcctcg ggtgcgagcc ccgtcgcctt
     6121 cgtctcgggg cggcggacgg gagtccacgc ccgacacgcg gaggcgccgt cgctcgacac
     6181 cgtcgtcggg cgggagatgc ccgacgtcga cgtggtcgtc gtcgagggct tccgcgagga
     6241 agcgggcccg aaggtcgagg tctgccgttc cgcgaccggc cgcgacgcgg tcgccgccgg
     6301 cgacccggag gtgctcgccg tgatgacgga ccgcgagact tctcacccct cctccgtccc
     6361 tcgtctgccg ctcggcgacg tcgatcggct cgtcccgctc gtcctcgacg ccctcggcct
     6421 cggaggtccc cgatgaaggc gatcctcgtc ccgtctcccg gcggcccgga agcgctcgtc
     6481 ctcggcgaga cgccggagcc ggtcctccgc gagggggagg tcttcgtccg cgtccgcgcg
     6541 acggccgtca accgtgccga cctcctccag gcggcgggga agtacccgcc gccgccgggg
     6601 gagtcggaga tcctcggcct cgaggcggcg ggcgtcgtcg aagagacggg cgagcgcgtc
     6661 ttctgcctcc tgcccggcgg cggctacgcg gagagggtcg ccgtcccgcg cgcgatgctg
     6721 atgccgatcc ccgaccggct ctcgttcgag gaggccgcgg cggtcccgga agcgtggttc
     6781 accgcgtacc tgaacctctt ccgcgaaggg gcgctcgccg ccggcgaggt cgtcctcctg
     6841 cacgcggcgg cctccggcgt cggcacggcg gcgatcccgc tcgcgaagga cgcgggggcg
     6901 cgcgtcgtcg cgaccgtccg ctctgccgcg aaggcgggcg cgctcgagtc gctcggcgcc
     6961 gacctcgtcg ttgacacgac ggcgcgcgac ttcgccgagg cgatcgagga gcggttcggc
     7021 aaggcgtccg tcggcctcgt cctcgacccg gtcggcggtg ccgcgctcgc ggggaacgtc
     7081 cgactcctcg cgcgcggcgg ccgcctcgtc ctcatcgcga cgatgggggg cggagcggcc
     7141 gagctcgacc tgcgcgccgt cctgacgaaa cggctccgga tcgtcggctc gacgctccgc
     7201 gcccggcccc tcgccgagaa ggccgacctg accgccgcct ttgtccgcga cgtcctcccc
     7261 ggcttcgccg acggccgctt ccggcccctc gtcgactccg tcttcccgat cgagcgggcc
     7321 gccgacgccc acaggcggat ggcgtcgaac gcgaacgtcg gcaagatcgt cctcacggtg
     7381 gcgtcgtgac gtaccgggcc gctgtcttcg acctcgacgg gacgctcctc gactcgtacg
     7441 tcgggatcca cgacgccctc tcgaccgtcc tcgagcactt cgcccgcccg gccgtcaccg
     7501 tcgaggaggc gaggcggctc gtcgggcacg gtctcgaggc gctcatcgcg aaggtcctcc
     7561 cggaggagct ccgcgccgag ggggcggagc gattccgttt ccgctacaag gtgaacgcgc
     7621 cgaagctgac gcgcctcatg cccgacgccg agctcgtcgt gtcggagctc tggcggcgcg
     7681 gcgtgaggct ctcgatcgcc tcgaacaagc cggccgtctt cagccgccag ctcctcgccg
     7741 gcttcggcct cgaggagctc ttcgccttcg tcggcgg
//
LOCUS       DAOTEZ010000181         7774 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_6634_length_7774_cov_5.691281, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000181 DAOTEZ010000000
VERSION     DAOTEZ010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7774
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6634_length_7774_cov_5.691281"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="P5164_10680"
     CDS             complement(<1..316)
                     /locus_tag="P5164_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HRY44402.1"
                     /translation="MAKIRPFPASRPAPARAREVASVPYDVIDTKEARALAEGNPGSF
                     LHVCRPEIDLPEGTSLYADEVYAKGRENLDRFESDGTLVPDPTPRLLVYRQTWRGQKQ
                     DGL"
     gene            393..635
                     /locus_tag="P5164_10685"
     CDS             393..635
                     /locus_tag="P5164_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRY44403.1"
                     /translation="MKLYVGNLPYEVDEPRLGEIFGAFGETISTTVVVDRTTGRSKGY
                     GFVEMADDDARKAILALNRTQLDGRTIWVNEARAKA"
     gene            complement(649..2538)
                     /locus_tag="P5164_10690"
     CDS             complement(649..2538)
                     /locus_tag="P5164_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY44404.1"
                     /translation="MTAPAEDAEGREKPPKVRRLKALVPDLLELARPRRKLLAVGFVL
                     MAAGRTAGLVLPASTKVLIDEVVAKSRNDLLAPLLLAIGAATLVQAATSFALAQLLSK
                     GAQRLIAELRTKVQSHVTRLPVAYFDGQKTGNLVSRVMNDVEGIRNLVGTGVVELAGG
                     LLTALLALGLMVTLSPVLTGLTLLFLGAFVVGLFAALGSLRPIFRERSKIHGEVSGRL
                     TETFAGIRVVKGYHGEAREDAVFGTGVSRLLSNVLRTITGTSALSAGATVLLGGVGAA
                     VMWVGTRQILSGALTVGGLFTYTILLGFLVAPVFQIVGIGSQLTEALAGLERTKEVLA
                     ESREDADPRRAVDVPPLRGEVVFEEVRFAYSEGKEVLHGVSFRAEPGSVTALVGPSGS
                     GKSTIIGLVAAFASPTAGRVLVDGVDLSTVRLSSYRSQLGVVLQESFLFDGTIRENVS
                     FSRPSATEGEVVAACRVARVDEFALGFEKGYETVVGERGVKLSGGQRQRVSIARALLA
                     DPRILILDEATSSLDTESEAFIQLGLAELLRGRTTFVIAHRLSTIRRADQILVVDGGA
                     IVERGRHDELMAAGGRYAEMVTRQAGIGVGLFGGPAEAPMDEEDGSSRPSPAGPFSLL
                     GPGRE"
     gene            complement(2535..3683)
                     /locus_tag="P5164_10695"
     CDS             complement(2535..3683)
                     /locus_tag="P5164_10695"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRY44405.1"
                     /translation="MKNVAVLGAGLVGSLVARDLAADGRWSVLSVDRSEHALGRLAGI
                     PNLATERADLASGAEVSRIASKVDVVVGAVPGFLGTQTVRTVLETGRPVADISFSPED
                     PFDLSPLAKSKGVPAIVDCGVSPGLSGLAVGRAAARLDSLEDVRIFVGGLPAARHWPF
                     EYRSVFSPTDVIEEYTRPSRCVEGGEVVIRPALSGVEPLDVPGVGTLEAFYTDGLRTL
                     LSTVKARNLYEKTLRYPGHAERMRMLRETGFFSGEPVAVDGVPVVPRRLTESLLFHAW
                     RRPEDEAELTFLRVVCEGRHGGRRVRRTFELLDRTDPRTGDTSMARTTGFPCATGARL
                     LLEGVFTTPGVFPPELLAGDDALYGRFLAELAARGVAVEETEEELVEG"
     gene            3790..4122
                     /locus_tag="P5164_10700"
     CDS             3790..4122
                     /locus_tag="P5164_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44406.1"
                     /translation="MIGASLFRSVARGLLAAGRTAFVLGALVALLHVTAPHDHASVSD
                     AQRCTACQLQRFDGGGTPEADPIPLAPPARTSAPVALAAVDLAPRAACTAPAPSRGPP
                     AISPSEPL"
     gene            4200..5753
                     /locus_tag="P5164_10705"
     CDS             4200..5753
                     /locus_tag="P5164_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRY44407.1"
                     /translation="MKTPLAGAIAIALALVPSIPAAGQAGPDPRALEEKVRALEARQA
                     ESEKALEKLKAALAAPPSAASAEELKRRLEALEEEQAATRAGLAELRAASSQKAPGPV
                     EPTGLVPKSLPLFGSSGPVSSGTSFNPSITVIPDVVWSRDDVKGEAGEILEEADGFHG
                     AHAEEGHDHGHGGLSQGFNLRETEIAFSASVDAYFDVQALFAVSEEGIEAEEVFFQTR
                     RLPWGLQLKGGKLLSGIGYANAHHPHQWDFADQNLAYELLLGGHGLNEKGLQLTWVPK
                     LPFYLKVGAELLQGENEKLASYVGPEEFPGLGEEGAPRVLSEKGGPRLFTGFVKVGPD
                     LGYSNALQVGFSLVSSRRHQEVHDEDADGVPEEVLDGTATLWGVDAVYKYDSPRTGGA
                     GDLVLQAEYLARKRNLDVLGTVDTAVFRQDGAYFQAVYGLLPRLQLGARWDVGGLTNE
                     RVRGGTKGAYDSSSRFTAALTFNPTEFSRIRAQYESASIRVDGEKETWGRFLLQLQLS
                     LGAHGAHAF"
     gene            5764..6648
                     /locus_tag="P5164_10710"
     CDS             5764..6648
                     /locus_tag="P5164_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HRY44408.1"
                     /translation="MKKTLLASALLLSALPSAAALQVVASTSSMGVLATAVGGDAVKV
                     TVLAPPDRDVHVLQARPSMMVALRRADLLVAVGAELEIGWLPAALKGAGNAKILPGAP
                     GYFEAAAQVPLLESGQAADRSQGDVHPMGNPHLYLDPVRMAKVAEALAERLASLDAPN
                     AARYRAGAAAFRAAVEARVPAWKARAAGAKGLLLFHKDVNYLAALLGVPILGYVEPLP
                     GIPPTASHLRELTGRFRGQKGAILFTDFQPGQGPEFLSRNLGWPKARLPLEPPVGAST
                     EAYLAHIDRWVDGVASGK"
     gene            6645..7211
                     /locus_tag="P5164_10715"
     CDS             6645..7211
                     /locus_tag="P5164_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY44409.1"
                     /translation="MSDEPILSYREVVAGYAKPVVGPVSFDLGPGDVLGLVGPNGCGK
                     STLIGALTGTSKVLSGEVVRKPGTTIAYQRQRPVRETDVPLRADELLRLTAATAEPPP
                     SLRPLLKTRVDRLSGGQFQLLTVWACLGGDARLVILDEPTNNMDPDAVAALSELLLSS
                     RGDRAVLVVTHDARFFTGVPARVLEVGR"
     gene            7208..>7774
                     /locus_tag="P5164_10720"
     CDS             7208..>7774
                     /locus_tag="P5164_10720"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRY44410.1"
                     /translation="MTFAQVFDPLFRVPLANGFLLALVLPLLGAYVRLRNEWLAALGL
                     AQVTAAGGVMAILIGVHPLAGSLGAAVVAAAIKGRTERSGNDVYAALLFFGWSAALLG
                     AANSTRGEELSHSLLEGQLYFTTWSNLGAALALVTIVATAGTWLSKRLLLEHFFPESL
                     AANGASPWKLHLAFDLLVAFSVALATESI"
BASE COUNT         1103 a         2955 c         2652 g         1064 t
ORIGIN      
        1 cgaggccgtc ctgcttctgt ccccgccagg tctggcggta gacgaggagg cggggagtcg
       61 ggtcggggac gagcgtcccg tccgactcga agcggtcgag gttctcgcgg cccttcgcgt
      121 agacctcatc ggcgtagagg gacgtcccct cggggaggtc gatctcgggg cggcagacgt
      181 gcaggaaaga gccgggattc ccttcggcga gggcccgggc ctccttcgtg tcgatgacgt
      241 cgtaaggaac ggacgcgacc tcgcgggcgc gggcgggagc gggacgggag gcggggaagg
      301 ggcggatctt cgccatgtcg ggacgagctt agcaggagcc attccgtcct tccctccccg
      361 ctccgcgagg cggtgtagga gaatccgacc ggatgaagct ctacgtcggg aacctcccct
      421 acgaggtcga cgagcctcgc ctcggcgaga tcttcggagc cttcggcgag acgatctcga
      481 cgacggtcgt cgtcgaccgg acgaccggtc gctccaaggg ctacggtttc gtcgagatgg
      541 ccgacgacga cgcccggaag gcgatcctcg ccctcaaccg gacccagctc gacggccgga
      601 cgatctgggt gaacgaggcc cgggcgaagg cctgaccggc cgcgcctctc actcccgacc
      661 ggggcccagg agcgagaacg ggccggccgg cgacggtctc gacgagccgt cctcctcgtc
      721 catcggcgcc tccgccggac cgccgaagag ccccaccccg attccggcct gccgcgtgac
      781 catctccgcg tagcggcctc cggcggccat cagctcgtcg tgacgccccc gctccacgat
      841 cgctcccccg tcgacgacga ggatctggtc ggccctccgg atcgtcgaga ggcgatgggc
      901 gatgacgaac gtggtccgcc cgcgcagcag ctccgcgagc cccagctgga tgaacgcctc
      961 cgactcggtg tcgaggctcg acgtcgcctc gtcgaggatc aggatccgcg ggtcggcgag
     1021 gagggcacgc gcgatcgaga cccgctggcg ctggcctccc gaaagcttca cgccccgctc
     1081 gccgacgacc gtctcgtagc ccttctcgaa gccgagggcg aactcgtcca ctctcgcgac
     1141 gcggcacgcg gcgacgacct ccccctcggt ggcggaaggc ctcgagaacg agacgttctc
     1201 ccggatggtg ccgtcgaaga ggaacgactc ctgcaggacc actccgagct gcgagcggta
     1261 gctcgagagc cggaccgtcg agaggtcgac accgtcgacg aggacgcgcc cggccgtcgg
     1321 cgaggcgaac gccgcgacga ggccgatgat cgtcgacttc cccgagccgg agggaccgac
     1381 gagcgcggtc acgctccccg gctccgcccg gaacgagacg ccgtggagga cctccttccc
     1441 ctcggaatac gcgaagcgga cctcctcgaa gacgacctcg ccgcggagag gagggacgtc
     1501 gacagcgcgc cgcgggtcgg cgtcctcccg ggactcggcg aggacctcct tcgtccgctc
     1561 gaggcccgcg agagcctcgg tcagctggct cccgatcccg acgatctgga agaccggagc
     1621 cacgaggaac ccgaggagga tcgtgtaggt gaagaggccg ccgacggtca gggcgcccga
     1681 caggatctga cgcgtcccga cccacatcac cgcggcgccc accccgccga ggaggaccgt
     1741 cgccccggcc gacagcgccg acgtccctgt gatcgtccgg aggacgttcg agaggaggcg
     1801 cgacaccccg gtcccgaaga cggcgtcctc tcgggcctcg ccgtggtacc ccttcacgac
     1861 gcggatgccg gcaaacgtct cggtaaggcg gcccgacacc tccccatgga tcttcgatcg
     1921 ctcgcgaaag atcgggcgca gggacccgag ggccgcgaag aggccgacca cgaaggcccc
     1981 gaggaaaagg agcgtcagcc ccgtcaggac ggggctgagc gtcaccatga ggccgagggc
     2041 gaggagcgcc gtcaggagtc cgcccgcgag ctcgacgacg cccgtgccga cgaggttccg
     2101 gatcccctcg acgtcgttca tcacgcgcga gacgaggttc cccgtcttct gtccgtcgaa
     2161 gtacgcgaca gggagccgcg tcacgtggga ctgcaccttc gtccggagct ccgcgatgag
     2221 ccgctgcgcc cccttcgaga ggagctgcgc gagcgcgaac gacgtcgccg cctggacgag
     2281 ggtcgccgcg ccgatcgcga ggaggagcgg cgcgaggagg tcgttgcgcg acttcgcgac
     2341 gacctcgtcg atcaggacct tcgtcgacgc gggaaggacg agccccgccg tccgtcccgc
     2401 ggccatcagg acgaagccaa ccgccaggag cttccggcgc ggccgcgcga gctcgaggag
     2461 gtccgggacg agcgccttca ggcgccggac cttcggcggc ttctcccgac cttccgcgtc
     2521 ctcggccggc gcggtcatcc ttcgacgagc tcttcctccg tctcctcgac ggcgaccccg
     2581 cgggccgcga gctcggcgag gaaacgtccg tagagcgcgt cgtcgcccgc caggagctcc
     2641 ggcgggaaga ccccgggcgt ggtgaacacc ccctcaagga ggaggcgcgc gccggtggcg
     2701 cacggaaatc cggtcgtccg cgccatggag gtgtcgcccg tcctcgggtc cgtccggtcg
     2761 aggagctcga acgtccggcg gacgcgccgg cctccgtggc gcccttcgca gacgacgcgc
     2821 aggaacgtca gctccgcctc gtcctcgggc cgccgccagg cgtggaagag gagcgactcc
     2881 gtcaggcggc gcgggacgac cggcacgccg tccaccgcga ccggctcgcc ggagaagaag
     2941 ccggtctcgc ggagcatccg catcctctcg gcgtggcccg gatagcggag cgtcttctcg
     3001 tacaggttcc gggccttcac ggtcgagagg agcgtccgga ggccgtccgt gtagaacgcc
     3061 tcgagcgtcc cgacccccgg gacgtcgagc ggctcgacgc ccgagagggc cgggcggatc
     3121 acgacctctc ccccttccac gcagcgggac ggccgcgtgt actcctcgat cacgtcggtg
     3181 ggcgagaaga ccgaccggta ctcgaacggc cagtgcctcg cggcggggag accgccgacg
     3241 aagatccgga cgtcctccag gctgtcgagc cgtgcggccg cccttcccac cgccagcccc
     3301 gagaggcccg gcgagacgcc gcagtcgacg atcgccggga ctcccttcga cttcgccagc
     3361 ggcgagaggt cgaacgggtc ctccggagag aaggagatgt cggcgaccgg ccggcccgtc
     3421 tcgaggacgg tccggaccgt ctgcgttccg aggaagcccg gcaccgcgcc gacgacgacg
     3481 tctaccttcg acgcgatccg ggagacttcg gccccgctcg ccaggtcggc gcgctccgtc
     3541 gcgaggttcg ggatgccggc gaggcgcccg agggcgtgct cgctccggtc gaccgagagg
     3601 acggaccagc ggccgtcggc ggcgaggtcc cgggcgacga gcgagccgac gaggcccgct
     3661 ccgaggacgg cgacgttctt catgggcgcg attatccccg tgccgcgccg gcgggagcct
     3721 ccgaccgcgc gaagccctct tcccccgctt gcttgacacc cccggcaccc ctccctaccc
     3781 ttcgcccgaa tgatcggggc ctccctcttc cggagcgtcg cgcgcgggct gctggccgcg
     3841 ggccgcaccg cgttcgtcct cggggccctc gtcgcgctcc tccacgtcac ggcgccgcac
     3901 gaccacgcga gcgtctcgga tgcgcagcgc tgtaccgcct gccagctcca gcgcttcgac
     3961 ggcggaggga cgccggaggc cgacccgatc ccgctcgcgc ccccggcccg gacgtcggca
     4021 cccgtcgccc tcgccgctgt cgacctggcg ccgcgtgccg cctgcacggc gccggccccc
     4081 tcccgcggac ctcccgcgat ctctccttcc gagccgctct agtccgctcg acctccgccc
     4141 cgaggggcgg agtcggcgct ccggccgcac cggcggcggg cgccccacgg agagacccga
     4201 tgaagacccc gctcgcgggc gcgatcgcga tcgcgctcgc tctcgtccct tcgatccccg
     4261 ccgccggcca ggccggcccc gatccccgcg ccctcgagga gaaggtccgc gccctggagg
     4321 cgcgccaggc cgagtccgag aaggcgctcg agaagctgaa ggccgcgctc gccgcgccgc
     4381 cttccgccgc ctcggccgag gagctgaagc gccggctcga ggcgctggag gaggagcagg
     4441 ccgcgacccg ggccggcctc gccgagctcc gggccgcctc gtcgcagaaa gccccgggcc
     4501 ccgtcgagcc gaccgggctc gtaccgaaga gcctcccgct cttcggctcc tccggcccgg
     4561 tctcctccgg cacctcgttc aacccttcga tcaccgtcat ccccgacgtc gtctggtccc
     4621 gcgacgacgt gaagggcgag gccggcgaga tcctcgaaga ggccgacgga ttccacggcg
     4681 cgcacgcgga agaagggcac gaccacgggc acggaggtct gtcccaggga ttcaacctcc
     4741 gcgagacgga gatcgcgttc tccgcctccg tcgacgccta cttcgacgtg caggcgctct
     4801 ttgccgtcag cgaggagggg atcgaggcgg aggaggtctt cttccagaca cgccggctcc
     4861 cgtggggcct ccagctcaag gggggcaagc tcctgagcgg gatcggctac gcgaacgcgc
     4921 accacccgca ccagtgggac ttcgccgacc agaacctcgc ctacgagctc ctcctcggag
     4981 ggcacggcct gaacgagaag gggctccagc tgacgtgggt cccgaagctg ccgttctacc
     5041 tgaaggtcgg ggcggagctg ctccaagggg agaacgagaa gctcgcgagc tacgtcggcc
     5101 ccgaggagtt cccgggtctc ggcgaagagg gggcgccccg cgtcctctcg gagaaaggtg
     5161 gaccgcgcct cttcaccggc ttcgtgaagg tcggcccgga cctgggctac tcgaacgccc
     5221 tgcaggtcgg cttctctctc gtctcgagcc ggcggcacca ggaggtccat gacgaggacg
     5281 ccgacggggt gccggaggag gtcctcgacg gcacggcgac gctctggggc gtcgacgccg
     5341 tctacaagta cgactcgccc cgcaccgggg gcgccggcga cctcgtcctc caggcggagt
     5401 acctcgcgcg gaagcggaac ctcgacgtcc tcggcaccgt cgacaccgcc gtcttccgcc
     5461 aggacggcgc ctacttccag gcggtttacg gcctcctccc ccgcctccag ctcggcgccc
     5521 gctgggacgt cggcggcctg acgaacgagc gggtccgtgg cgggacgaag ggcgcgtacg
     5581 actcctcgag ccgcttcacg gcggcgctca cgttcaatcc gaccgagttc tcgcgcatcc
     5641 gcgcgcagta cgagagcgcc tcgatccgcg tcgacgggga gaaggagacc tggggacgct
     5701 ttctcctcca gctccagctc tcgctcggcg cccacggcgc ccatgcgttc tgaggagacg
     5761 gacttgaaga agactctgct ggcctcggcg ctcctcctct ccgccctccc ctccgccgcg
     5821 gcgctccagg tcgtcgcctc gacctcgagc atgggggtcc ttgcgaccgc cgtcggcggc
     5881 gacgccgtga aagtgacggt cctcgccccg cccgatcggg acgtccacgt cctgcaggcc
     5941 cgcccgagca tgatggtggc cctccggcgg gccgacctcc tcgtcgccgt cggcgccgag
     6001 ctcgagatcg gctggctgcc cgccgccctg aagggggccg ggaacgcgaa gatcctcccc
     6061 ggcgctccgg gctacttcga ggcggccgcc caggtcccgc tcctcgagtc cggtcaggcc
     6121 gccgaccgct cgcagggcga cgtccacccg atggggaacc cccatcttta cctcgacccc
     6181 gtccggatgg cgaaggtcgc cgaggcgctc gccgaacggc tcgcctccct cgacgctccg
     6241 aacgccgccc gctatcgcgc cggcgccgcc gcgttccgcg ccgccgtcga agcgcgagtg
     6301 cccgcatgga aggcaagggc cgccggagcg aaggggctcc tcctcttcca caaggacgtc
     6361 aactacctcg ccgccctcct cggcgtcccg atcctcggct acgtcgagcc gctgccgggg
     6421 atcccgccga ccgcctcgca tctccgggag ctgacgggcc gcttcagggg acagaagggg
     6481 gcgatcctct tcaccgactt ccagccgggc caggggccgg agttcctctc ccgcaacctc
     6541 ggctggccga aggcgcgact cccgctggag ccgcccgtcg gcgcttccac ggaggcttat
     6601 ctcgcgcaca tcgaccgctg ggtggacgga gtagcctcgg gaaagtgagc gacgaaccga
     6661 tcctctccta ccgggaggtc gtcgcgggct acgcgaagcc cgtcgtcggc cccgtttcgt
     6721 tcgacctcgg ccccggcgac gtcctcggcc tcgtcgggcc gaacggctgc ggcaagtcga
     6781 ccctcatcgg cgcgctgacc ggcacgtcga aggtcctctc gggcgaggtc gtccggaagc
     6841 cggggacgac gatcgcctac cagcgccagc gtccggtccg ggagacggac gtgccgctcc
     6901 gcgccgacga gctcctccgt ctcaccgccg cgacggcgga gccgcctcct tccctccggc
     6961 ccctcctgaa gacccgtgtc gacaggctct cgggcgggca gttccagctc cttacggtct
     7021 gggcctgcct cggcggcgac gcccgcctcg tcatcctcga cgagccgacg aacaacatgg
     7081 accccgacgc cgtcgccgct ctctccgagc tcctcctctc ctcccgtgga gaccgcgcgg
     7141 tcctcgtcgt cacgcacgac gcccggttct tcaccggggt ccccgcccgc gtcctggagg
     7201 tgggccggtg acgttcgcgc aggtcttcga ccccctcttc cgcgtcccgc tcgcgaacgg
     7261 cttcctcctc gccctcgtcc tgccgctcct cggcgcctat gtgaggctcc gcaacgagtg
     7321 gctcgccgcg ctcggcctcg cgcaggtgac cgccgccggc ggggtgatgg cgatcctcat
     7381 cggcgtccac cctctggccg gatcgctcgg cgcggccgtc gtcgccgccg cgatcaaggg
     7441 gcgcacggag cggtcgggga acgacgtcta cgccgccctc ctcttcttcg gctggagcgc
     7501 cgccctcctc ggcgcggcga acagcacgcg cggtgaggag ctctcgcact cgctcctcga
     7561 ggggcagctc tacttcacga cgtggtcgaa cctcggcgcg gcgctcgccc tcgtcacgat
     7621 cgtcgcgacg gcagggacct ggctctcgaa gcggctcctc ctcgagcact tcttcccgga
     7681 gagcctcgct gcgaacgggg cttccccctg gaagctccac ctcgcgttcg acctcctcgt
     7741 cgccttctcg gtcgccctcg ccacggagtc gatc
//
LOCUS       DAOTEZ010000182         7757 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_6653_length_7757_cov_6.531550, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000182 DAOTEZ010000000
VERSION     DAOTEZ010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7757
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6653_length_7757_cov_6.531550"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="P5164_10725"
     CDS             complement(<1..407)
                     /locus_tag="P5164_10725"
                     /inference="COORDINATES: protein motif:HMM:NF012536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HRY44411.1"
                     /translation="MTTSYGTIGTRLHRHLFETERRYPHATGELSALLSQLALAGKRI
                     ARVLSRAGLAEVLGPSGVTDVEKERLQKLDTLANEIFLEAFAYGQLVPTVVTEHMDLP
                     ARLPENETVAASAGKYVVFVDPLDGASNLDINGA"
     gene            complement(404..1393)
                     /locus_tag="P5164_10730"
     CDS             complement(404..1393)
                     /locus_tag="P5164_10730"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HRY44412.1"
                     /translation="MLLAGDVGGTNLRLALYDLVDGALVERRRTRVPTRDVTGLVEAA
                     AAFVGGEPGLEGAAFGVAGPVRDGKAEGTNVAFAIDAAELGSSLGVPAVVLNDLEANA
                     WGLDELAPEDFEVLNRGEADARGNGALISAGTGLGEAILFRTPSGFVPMPSEGGHASF
                     APRNDEEIELLKTLRLRHGHVSWERVVSGPGLGTLYRFERQLSGVPEPEWLAREITAS
                     GDPAPVVSRAALDETDPVCQRTLHRFVSLFGAEAGNLALKALATGGVFLGGGIAPKIL
                     PLLRDGFFTAFTAKGRFAPVLARIPIRVVRNDSCAILGAARAAARAARGEVTT"
     gene            complement(1396..2121)
                     /gene="pgl"
                     /locus_tag="P5164_10735"
     CDS             complement(1396..2121)
                     /gene="pgl"
                     /locus_tag="P5164_10735"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HRY44413.1"
                     /translation="MTTTQVTVFPSASALAEGVAELFAVRASEAVAERGRFRAALAGG
                     TTPLAAYRRLAAAPWAELVPWGSVELFFGDERSVGECDGERNDAAARDALLLRVPIPP
                     ENVHPVPALAPDGAERYEALLRARLGAPAPAIPVLDLVLLGLGEDGHTASLFPGHPAA
                     GETERLVVRVDGAPKPPPSRVTMTLPLLNAARVVAFVVSGTGKSAALARVLAGDRALP
                     AARVEPLDGERLFLLDRAAAGEV"
     gene            complement(2135..3637)
                     /gene="zwf"
                     /locus_tag="P5164_10740"
     CDS             complement(2135..3637)
                     /gene="zwf"
                     /locus_tag="P5164_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HRY44414.1"
                     /translation="MSPAPAPAATIVIFGGSGDLARRKLVPALFELHRQGLLAPETAI
                     VGFARSVGSDEAWRDDLDAALDEHGRGGADAGARRSFAARLHAVRGDLRSDASYGELK
                     SRLEAIEAERGLPGNRLYYLAIPPSGIGDAARRLGAAGLVHPVEGDGPFSRLVVEKPF
                     GHDLASAHALNEAVHASFRERQVFRIDHYLGKETTQNILVFRLGNGIFEPLWNRRYVD
                     HVQITVAETIGVEGRGKFFEEAGIFRDVVQNHLLQLLCLVAMEPPVAFEANVVRDEKV
                     KVLRSVRPPSPQEVLRDTVRGQYAAGTIGGKAVPGYREERDVAAGSVRETYAAWKLQI
                     ENWRWAGVPFYLRAGKRLPKRVTEIAITFKTPPIALFRHAGCAVGEPNVLVLRIQPDE
                     GIALRIGAKRPGPTMRVETVKMDFRYAEQFGEATPDAYERLLLDALNGDGTLFARRDE
                     VEAAWELVEGVLDVWAESPPPDLPNYEAGTWGPEAATELLARYGRRWRRP"
     gene            complement(3648..4808)
                     /gene="tal"
                     /locus_tag="P5164_10745"
     CDS             complement(3648..4808)
                     /gene="tal"
                     /locus_tag="P5164_10745"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HRY44415.1"
                     /translation="MNRSVDYRKRGQAVWLDSISARMIATGELAGRIAAGSVYGVTSN
                     PTIFGQAIGKGDGDYEDRIANLADEGKDAFGLYDELTRGDIAAGADLFAAQHAANPED
                     GWISLEVLPSLAFDIEGTIAEAKRLQRLLGRPNVFIKVPATPAGCVAVRQLIAAGVSV
                     NVTLMFNRKHYRDVAHAYQAGIEALVAAGGDPSKVASVASFFVSRVDTLVDKLLGERA
                     AIASGALKQKLEGLTGKAALANAKVVYQDYLAAFGTDAWKALAAKGARVQRPLWASTG
                     TKNPAYSDLLYVENLVGPDTVNTLPEKTLLALLDHGRDPGDTVLEGVDEARRTLDELA
                     ELGLDVEALGEKLQGEGVVLFERSYDELLAVVEERRRAALRESAQTVRIRPQ"
     gene            4994..6256
                     /locus_tag="P5164_10750"
     CDS             4994..6256
                     /locus_tag="P5164_10750"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRY44416.1"
                     /translation="MSRLARILLVAASASVPLRAAEPPSPPPPLSLSDVLRGAAERAP
                     LAAEAEARRAVAEADAAKARRLPNPEASLRVENWRRGSEARPFDAGTDLDVVAELAQP
                     LPVFGSWSARKGETAALARAAGAVARGEREALLLEAARLYLAVLRGRDLVTALAESRD
                     VLGTMVATMEKRVAEGWSAEGDLLKLRAERERAETLLSRATLERDEAAGRLAALLGEE
                     TPVDPARLSRLAPAPPPDGAPEALAREAAAARPAVVEAREALEAARAALRLEKALRAP
                     EPSLSAGYKRTGGLDTALAGIALPLPLFDANGAGIARASARLRGAEARLRAAELEAAA
                     DVAARLRAARAYAERAARAETELLAAALGARSAARAAFREGASEVLPLVDAERVTAEA
                     VRETLDLTVDAVLSSLAARLALGQEVVP"
     gene            6253..7521
                     /locus_tag="P5164_10755"
     CDS             6253..7521
                     /locus_tag="P5164_10755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY44417.1"
                     /translation="MSTNPGAPAPEGFWKRHQRHLPRRLPRVLLAVGLLLVPAVVVIR
                     LFTAKPGEEAAPAAAATDAVTLGEKSIGLAGIVVAEARSVTRAATFEAPAVLALDEAR
                     TARIGSLVEGNVVRVLAEVGDRVGRGAVLAEMNSVVVHVAWADYRKAVSERRRRETEL
                     AYSTQAAERAGRLHADKALSLQERQRADADLVAAREELDRANTEVRRAEEALEHLGVT
                     NAEDPSGESGESIPVRAPLAGVVLEKFVTAGTAATPGTPLFVVSDLSALWAMAEVDET
                     RIPLVASGRPAKLFVAAWPDRPFDAKVTFVGDAVNPKTRRVTVRCAVPNPGGLLKPEM
                     YARVSLGESAPRPMVVVPEAAIQEMDGKPHVFVAGEKGRFAKREVALGATSEGLVEVR
                     SGVKAGEKVATTGSFLLKSQLLSASAPAEE"
     gene            7523..>7757
                     /locus_tag="P5164_10760"
     CDS             7523..>7757
                     /locus_tag="P5164_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44418.1"
                     /translation="MAAGERGERKHRGEAEAVPRPKGIERFVAAAYRRRLAVHAMVAF
                     LVLSGLWAWQELPVEAFPDLTNNQAVVVTEAQGL"
BASE COUNT          970 a         2855 c         2941 g          991 t
ORIGIN      
        1 gcgccgttga tgtcgaggtt cgaggcgccg tcgagcgggt cgacgaagac gacgtacttc
       61 cccgccgagg cggcgaccgt ctcgttctcg gggaggcgcg ccgggaggtc catgtgctcc
      121 gtgacgacgg tcgggacgag ctgcccgtag gcgaaggcct cgaggaagat ctcgttcgcg
      181 agggtgtcga gcttctggag gcgctccttc tccacgtcgg tcacgccgga gggcccgagc
      241 acctcggcga ggccggcgcg ggagaggacg cgcgcgatcc tcttccccgc gagcgcgagc
      301 tgggagagga gcgccgagag ctctccggtg gcgtgcgggt agcgcctctc cgtctcgaag
      361 aggtgccggt ggagccgggt cccgatcgtt ccgtacgacg tcgtcacgtc gtcacctctc
      421 cgcgggccgc cctggcggcc gcccgcgcgg cgccgaggat ggcacaggag tcgttccgga
      481 cgacgcggat cgggattcgg gcgagcaccg gcgcgaagcg ccccttggcc gtgaaggcgg
      541 tgaagaaacc gtccctgagg agcgggagga tcttcggggc gatgccaccg ccgaggaaga
      601 cgccaccggt ggcgagcgcc ttcagggcga ggttccccgc ctcggcgccg aagagggaga
      661 cgaaacggtg gagcgtccgc tggcagacgg ggtccgtctc gtcgagcgcc gcgcggctga
      721 cgacgggcgc agggtctccc gaggcggtga tctcgcgcgc gagccactcc ggctcgggga
      781 cgcccgagag ctggcgctcg aaacggtaga gggtgccgag gcccggcccg gagacgacgc
      841 gctcccagga gacgtggccg tgccggaggc ggagcgtctt caggagctcg atctcctcgt
      901 cgttccgcgg ggcgaaggag gcgtgcccgc cctccgacgg catcggcacg aacccggacg
      961 gcgtacggaa gaggatcgcc tcgccgaggc cggtgcccgc ggaaatgaga gcgccgtttc
     1021 cgcgcgcgtc ggcctcgccc cggttcagga cctcgaagtc ttccggggcg agctcgtcga
     1081 gcccccaggc gttcgcctcg aggtcgttca ggaccaccgc ggggacgccg agcgaggagc
     1141 ccagctcggc ggcgtcgatc gcgaacgcga cgttcgtccc ttccgccttc ccgtcgcgga
     1201 ccgggccggc cacgccgaac gccgcgccct cgaggcccgg ctcgccgccg acgaaggcgg
     1261 ccgcggcctc gacgaggccc gtcacgtcgc gcgtcggcac gcgcgtgcgg cggcgctcga
     1321 cgagcgcgcc gtcaacgagg tcgtagaggg cgaggcgaag gttcgtcccg ccgacgtctc
     1381 ccgcgagcag catcgtcaca cctcccccgc ggcggcgcgg tcgagcagga agagcctctc
     1441 gccgtcgaga ggctcgacgc gagcggcggg gagagcgcgg tctcccgcga ggacccgggc
     1501 gagcgccgcg ctcttgcccg tcccggagac gacgaacgcg acgacgcgcg ccgcgttcag
     1561 gagcgggagg gtcatcgtga cgcgcgacgg cggcggcttc ggcgcgccgt cgaccctcac
     1621 gacgagccgc tcggtctcgc cggccgccgg gtgtcccggg aagagcgagg ccgtgtggcc
     1681 gtcctcgccg aggccgagga ggacgaggtc gaggaccggg atcgcgggcg ccggcgcgcc
     1741 gagccgcgcc cggaggagcg cctcgtagcg ctccgctccg tccggcgcga gggccgggac
     1801 cgggtggacg ttctcgggcg ggatcgggac gcggaggagg agcgcgtccc gcgcggcggc
     1861 gtcgttccgc tccccgtcgc actcgccgac ggagcgttcg tcgccgaaga agagctcgac
     1921 ggagccccac gggacgagct cggcccaggg cgcggccgcg aggcgccggt aggcggcgag
     1981 cggggtcgtg cccccggcga gcgccgcgcg gaaccgcccg cgctcggcga ccgcctccga
     2041 agcgcggacg gcgaagagct cggcgacccc ctccgcgagg gccgacgccg acgggaagac
     2101 cgtgacctgc gtcgtcgtca cgcgctcgct cccgctacgg ccgccgccac cgccggccgt
     2161 accgcgcgag aagctcggtc gccgcctccg ggccccacgt ccccgcctcg tagttcggca
     2221 ggtcgggcgg ggggctctcg gcccagacgt cgaggacccc ctcgacgagc tcccacgccg
     2281 cctccacctc gtcgcggcgg gcgaagaggg tgccgtcgcc gttcagagcg tcgaggagga
     2341 gccgctcgta cgcgtcgggc gtcgcctcgc cgaactgctc cgcgtagcgg aagtccatct
     2401 tcaccgtctc gacgcgcatc gtcggcccgg gacgcttggc gccgatccgg agcgcgatcc
     2461 cctcgtcggg ctggatccgg aggacgagga cgttcggctc gccgacggcg cagcccgcgt
     2521 gccggaagag ggcgatgggc ggcgtcttga acgtgatcgc gatctcggtg acccgcttcg
     2581 gcagccgctt gccggcccgc aggtagaacg ggacgccggc ccagcgccag ttctcgatct
     2641 ggagcttcca ggcggcgtag gtctcgcgga cggagccggc ggcgacgtcg cgttcctcgc
     2701 ggtagccggg gacggccttc ccgccgatcg tccccgcggc gtactgcccg cgcaccgtgt
     2761 cgcgcaggac ctcctgaggc gacggcggcc ggaccgagcg gaggaccttc accttctcgt
     2821 cgcggacgac gttcgcctcg aacgcgacgg gcggctccat cgccacgagg cagaggagct
     2881 ggaggaggtg gttctggacg acgtcccgga agatccccgc ctcctcgaag aacttcccgc
     2941 gcccctcgac gccgatcgtc tcggcgaccg tgatctggac gtggtcgacg taccgccggt
     3001 tccagagcgg ctcgaagatg ccgtttccga ggcggaagac gaggatgttc tgcgtcgtct
     3061 ccttgccgag gtagtggtcg atccggaaga cctgccgctc gcggaaggag gcgtggaccg
     3121 cctcgttgag ggcgtgcgcc gaggcgaggt cgtggccgaa cggcttctcg acgacgaggc
     3181 gcgagaaggg gccgtcccct tcgaccgggt ggacgagccc ggccgcgccg aggcgccggg
     3241 ccgcgtcgcc gatcccggac ggcgggatcg cgaggtagta gagccggttt cccggcaggc
     3301 cgcgctccgc ctcgatcgcc tcgaggcggc tcttcagctc cccatagctc gcgtcgcttc
     3361 gcaggtcgcc ccggacggcg tggagccggg ccgcgaacga tcgccgcgcc ccggcgtcgg
     3421 cgccgccccg tccgtgctcg tcgagggcgg cgtcgaggtc gtcgcgccac gcctcgtcgg
     3481 agccgacgga ccgggcgaag ccgacgatcg ccgtctccgg ggcgaggagc ccctggcgat
     3541 ggagctcgaa gagggccggg acgagcttgc ggcgcgcgag gtcgcccgag cctccgaaga
     3601 tgacgatcgt cgccgcgggg gccggggcgg ggctcacgcg ccttccgtca ctgaggccgg
     3661 atcctcaccg tctgcgccga ttcccggagg gcggcgcgcc gcctctcctc gacgacggcg
     3721 aggagctcgt cgtacgaacg ctcgaagagg acgactccct ccccctggag cttctcgccg
     3781 agcgcctcga cgtcgaggcc gagctcggcc agctcgtcga gcgtccggcg cgcctcgtcg
     3841 accccctcga ggacggtgtc gccggggtcg cggccgtggt cgaggagcgc gaggagggtc
     3901 ttctcgggaa gggtgttcac ggtgtcgggg ccgacgaggt tctcgacgta gaggaggtcg
     3961 gagtaggccg ggttcttcgt cccggtggag gcccagagcg ggcgctggac gcgggcgccc
     4021 ttcgccgcga gcgccttcca ggcgtccgtc ccgaaggccg cgaggtagtc ctggtagacg
     4081 accttcgcgt tcgcgagggc cgccttcccc gtcagcccct cgagcttctg cttcagggcg
     4141 ccggaggcga tcgccgcccg ctcgccgagg agcttgtcga cgagcgtgtc gacgcgcgag
     4201 acgaagaacg acgcgacgga ggcgaccttc gacgggtcgc cgccggcggc gaccagggcc
     4261 tcgatccccg cctggtaggc gtgggcgacg tcgcggtagt gctttcggtt gaacatgagc
     4321 gtcacgttca cggagacgcc cgcggcgatc agctggcgga cggcgacgca gccggccggc
     4381 gtcgccggga ccttgatgaa gacgttcggg cgcccgagga ggcgttggag gcgcttcgcc
     4441 tcggcgatcg tcccctcgat gtcgaacgcg aggctcggaa ggacctcgag cgagatccag
     4501 ccgtcctcgg ggttcgcagc gtgctgcgcg gcgaagaggt cggcgccggc ggcgatgtcg
     4561 ccgcgcgtca gctcgtcgta gaggccgaac gcgtccttcc cctcgtcggc gaggttcgcg
     4621 atccggtcct cgtagtcgcc gtctcccttg ccgatcgcct ggccgaagat cgtcgggttc
     4681 gaggtgacgc cgtagacgga gccggccgcg atgcggcccg cgagctctcc cgtagcgatc
     4741 atccgggccg agatgctgtc gagccagacg gcctggcccc gcttccggta gtcgacggag
     4801 cgattcattc gggtcctcct gggatgtcgg gcggccgggc cgtcccgggg gttgccggat
     4861 cgtataccgg cgtcgcgacg ccctccccgc gccgcaggac ggcgcccccg gggccggccg
     4921 gcgcggctcc gatcccgagc gctccggttt cgttctggcc ccgttccccc ggcggcgcta
     4981 ctctggctga tcgatgagcc ggctcgcccg catcctcctc gtcgccgcgt cggcctccgt
     5041 cccgcttcga gccgcggagc cgccgtctcc gcccccgccc ctctccctct ccgacgtcct
     5101 gcgcggagcc gcggaacgcg cgcccctcgc cgcggaggcg gaggcgcggc gcgccgtcgc
     5161 cgaggccgac gccgcgaagg cccgccgcct cccgaacccg gaggcctccc ttcgcgtcga
     5221 gaactggagg cgcgggagcg aggcgcggcc gttcgacgcc gggacggacc tcgacgtcgt
     5281 cgccgagctc gctcagccgc tccccgtctt cggctcctgg tccgcacgaa agggcgagac
     5341 ggccgccctc gcccgcgccg cgggcgccgt ggcgcgggga gagcgggagg ccctcctcct
     5401 ggaagcggct cgcctctacc tcgccgtgct ccggggccgg gacctcgtca cggccctcgc
     5461 ggagagccgc gacgtcctcg ggacgatggt cgcgacgatg gagaagcggg tcgccgaggg
     5521 atggtcggcc gagggggacc tcctgaagct gcgcgccgag cgggagcgcg ccgagacgct
     5581 cctctcgcgg gcgacgctcg agagggacga ggccgccggc cgcctcgccg ccctgctcgg
     5641 cgaggagacg cccgtcgacc cggcgcggct ctcgcggctc gcgccggcgc cgcccccgga
     5701 cggggcgccg gaggcgctcg cccgggaagc cgccgccgcc cggcccgccg tcgtcgaggc
     5761 gcgcgaggcc ctcgaggccg cccgcgccgc gctccggctc gagaaggccc tgcgagcgcc
     5821 ggagccgtcc ctctcggccg gctacaagag gacgggaggg ctcgacacgg ccctcgccgg
     5881 catcgccctc ccgctcccgc tgttcgacgc gaacggcgcc ggaatcgcac gggcctccgc
     5941 ccgcctccgc ggcgccgagg cccgcctccg cgccgcggag ctcgaggccg ctgcggacgt
     6001 cgccgcccgc ctccgcgccg cccgggcgta cgcggagagg gcggcgcgcg cggagacgga
     6061 gctcctcgcc gccgccctcg gcgcgcggtc cgcagcccgc gccgccttcc gcgagggggc
     6121 ctccgaggtc ctcccgctcg tcgacgccga gcgggtcacg gcggaagccg tccgcgagac
     6181 cctcgacctg accgtcgacg cggtcctctc ctccctcgcc gcacgcctcg ccctcggaca
     6241 ggaggtcgtc ccgtgagcac gaaccccgga gcacccgcgc cggaaggctt ctggaagcgc
     6301 caccagcggc acctgccgcg ccgcctcccc cgcgtcctcc tggccgtcgg cctgcttctc
     6361 gtccccgcgg tcgtcgtgat ccgcctcttc acggcgaagc ccggagaaga ggccgctccc
     6421 gccgccgccg cgaccgacgc cgtcactctc ggagagaagt ccatcggcct cgccgggatc
     6481 gtggtcgccg aggcgcggag cgtgacgcgg gcggcgacgt tcgaggcgcc cgccgtcctc
     6541 gcgctcgacg aggcgcggac ggcccggatc ggctcgctcg tcgaggggaa cgtcgtccgc
     6601 gtcctcgccg aggtcggcga ccgcgtcggt cgcggggccg tcctggccga gatgaacagc
     6661 gtcgtcgtcc acgtcgcctg ggccgactac cggaaggcgg tgtcggagcg gcgtcgccgg
     6721 gagacggagc tcgcctactc gactcaggcc gcggagcgcg ccggacggct ccacgccgac
     6781 aaggcgctct cgctccagga gcggcagcgg gccgacgcgg acctcgtcgc cgcgcgcgag
     6841 gagctcgacc gggcgaacac ggaggtgcgg cgcgccgagg aggcgctcga gcacctcggc
     6901 gtgacgaacg ccgaggaccc gagcggcgag tcgggcgagt cgatccccgt ccgcgccccg
     6961 ctcgccgggg tcgtcctcga gaagttcgtc acggccggga ccgccgccac gcccgggacg
     7021 cctctcttcg tcgtgagcga cctctccgcc ctctgggcga tggccgaggt ggacgagacg
     7081 cggatcccgc tcgtcgcttc cggacgcccc gcgaagctct tcgtcgccgc gtggcccgac
     7141 cgccccttcg acgcgaaggt gacgttcgtc ggagacgccg tgaacccgaa gacgcgccgc
     7201 gtgacggttc gctgcgcggt cccgaacccc ggcgggctgc tgaagcccga gatgtacgcc
     7261 cgggtctcgc tcggcgagtc ggccccgcgg ccgatggtcg tggtgccgga ggccgcgatc
     7321 caggagatgg acggcaagcc gcacgtcttc gtcgccgggg agaaggggcg cttcgcgaag
     7381 cgggaggtcg cgctcggcgc gacgagcgag gggctcgtcg aggtccgctc cggcgtcaag
     7441 gccggcgaga aggtcgcgac gacggggagc ttcctcctga agtcgcagct tctctcggcc
     7501 tcggcgccgg cggaggagtg acgtggcggc cggagagcgg ggcgagcgga agcaccgcgg
     7561 cgaggcggag gcggtgccgc gtccgaaggg gatcgagcgg ttcgtcgcgg cggcttaccg
     7621 gcgaaggctc gccgtccacg cgatggtcgc gttcctcgtc ctctcgggcc tctgggcctg
     7681 gcaggagctc cccgtcgagg ccttccccga cctgacgaac aaccaggcgg tcgtcgtgac
     7741 cgaggcgcag ggcctct
//
LOCUS       DAOTEZ010000183         7746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_6662_length_7746_cov_5.940840, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000183 DAOTEZ010000000
VERSION     DAOTEZ010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7746
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6662_length_7746_cov_5.940840"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /gene="tilS"
                     /locus_tag="P5164_10765"
     CDS             complement(<1..858)
                     /gene="tilS"
                     /locus_tag="P5164_10765"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRY44419.1"
                     /translation="MQKPVPPGRSEPLPDAFARHVASSGVLDGAAAFVAACSGGSDST
                     ALALLLSAEARRREVPIVLFHVDHQLRGVEGHRDARAAADLAASLGASFVFRSFPVRD
                     LRETGESLESAARRLRYESLLALGRDLGPGTLLGTGHTLEDQAETVLLHLERRLGRSR
                     GGIRPRREDGVVRPLLPFRRGELREWLRSEGVPWREDASNADERFARNRVRRQLLPGL
                     EARWPGATVRLARAGEALTARLAGLDAAIDAALGESALPLEGPWPRRLFERLGREAAG
                     RLLIRAAGGT"
     gene            complement(897..972)
                     /locus_tag="P5164_10770"
     tRNA            complement(897..972)
                     /locus_tag="P5164_10770"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(937..939),aa:Met,seq:cat)
     gene            1028..1249
                     /locus_tag="P5164_10775"
     CDS             1028..1249
                     /locus_tag="P5164_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44420.1"
                     /translation="MPFLDEDEGVRADQCSRCGRICEPAEMETCPVCGRRYCSYCRYR
                     VGSRSYCSRPCGDSFFFGGDVDDEGLSEE"
     gene            1274..5155
                     /locus_tag="P5164_10780"
     CDS             1274..5155
                     /locus_tag="P5164_10780"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HRY44421.1"
                     /translation="MTEVLLLFLGMAVLLAPIVSLVVSLLGRSRLTKAEERLGELERS
                     RSALESELRALRVEIGKRREGVAEAPPPAPEGEARPPVPPAAAVSAPVPAPSRLEDTD
                     RITPLPVPPYPASPPPPPVPAPAAVPPPAPTLPAAPPVPPAASVAVPPVPPTFAVPPL
                     PAAASAGAASRPASPAAAGRAPAPTVPAPAPPRPAPPPARPASAPAAPPKAPFDWESL
                     VGVKLFSWVAAVALVVAAVLFLRYSIDHGWLTPPLRMAIGLLTGIGLLVACELKAARR
                     YPVTANALDGAAIAVLFSTFFAAHALWKLVPALPTFGLMALVTGAAVLLSVRRDSIFI
                     ALLGLVGGFATPALLSTGEDRPFGLFGYLLLLNAGLAWVAHRKKWPVLTVLCALLTAI
                     YQWGWVAKFLTEGKLGIAAGVFLVFPLVLYAGLALGKPEEKGADGKAPLFEGAARLAG
                     LLPLLFALFLAAVPAYGARHGLLFGFLFLLAAGLFAVAVWRGPSALHLAGGLATLLVF
                     VVWAFRSYHPGAWPAILAFLGLFVAFFLAAPFVAEKAGRPFDAPGRRAAYAAPLLLSL
                     APVLVSEEPRAASPALLFGVVFVLLALCAAYAIRREEGGVFFSGAFFALLAQAVWSAK
                     HLTPERLLPGLALYGVFSLFYLGVPLLARRLRKELKPGGLSVVLLFAALALLLFLAAG
                     PVAASALWGLALLLAVLNVGLFAEGAAGRYPVATILGVLLSWGVLAVWWRSVPVGTLL
                     VPALLVVLGFALVVLGGSAWARAKTGAGATTGPDGIHLGLVGHLFLLFVATQPPLAWP
                     PAPLLGVLLVLDLALAAAAFWSGLGALLAAALVLSQIVLLVFVAAVPPAPTVGLISAL
                     GVAALGLLARALAPRAPRGGEGLRLAVAQGAAAALLVGQVVALFAAGARGRPGLPLLV
                     AAVLLLAGALLAVAAREGWYGLSVLAAALGFFTVHLQAAAQPGADVAVPRLLFAGLVW
                     LLFLVHAPLAARRAPSARAPWVTAVLASGAFFLLARRSFSEAGWDGFVGALPVAIAAA
                     LALLVLSLLKIEAPAARDLGRLALVAGAALAFVTVAIPLQLDKEWITIGWALEAAALA
                     WLYTRVPHRGLLLASGGLAAVVFVRLVLNEAVLSYHARSATPIWNWYLYTYLAAAVAL
                     FVAARFLRGTADDLLDGRLRVSTADVVFGTVLLFALVNIEIADWFAEGPTLTFHLARG
                     TSLAQDLAYTLAWAVFGVGLLAAGIVGRSRAARAAALGLLVVTVLKAFLHDLGRLGGL
                     YRVASFVGLAISLSLVAIAIQKFVLRPREKA"
     gene            5152..7203
                     /locus_tag="P5164_10785"
     CDS             5152..7203
                     /locus_tag="P5164_10785"
                     /inference="COORDINATES: protein motif:HMM:NF024562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 family protein"
                     /protein_id="HRY44422.1"
                     /translation="MKPTRLLFAAGLLAAALPGAAEEGVRPVPSFRHERAVLPGAPGP
                     NRLDVDVSLLAGSEARTAGGLLSDLRLHDAAGREVPHLLVSPGRAEPEWLSATLLPIA
                     ATKTASGFEADLGALRRVDRLRVSGIPAPFLKRLRLEGSGDRARWALLAEEGTLFDLP
                     EEGLSRTVVAFAAGEHRYFRLVWNDAKSGRVPLPSRVEARAAGTGGAPEALRAEVPFE
                     RRESEPGRSRYRLRLPAAGLPISALEVGVRSGNVLREARVLESRLSAGRLVPYELGAA
                     TLRRAEREDAAAAEMTISVAPPAEAELELVVEDAGNPPLELASVTALFEGLPFVYFES
                     PDGAPLAARYGARALERPRYDLEAARSAVTRRGAAPPARAAWGEARPRPASAAEAAVA
                     PSVPAGAALDRTSFRVSRAIPPGPPGLTALRLDAAVLAQSPSLADVRIVSSDDRQVPY
                     LLETLGEPLVVRLPAPQSTGDPRPRAGEGAEGRLSFHRIDLSYETLPATRLELATTAR
                     VFARNVVVYRERLGGRPETAGTFTAIASSAWQSADPAREAPRLVLDLPPGTGGTLFVS
                     IDDGDNAPLPLAGASLLLPAHRLRFVREDGALALLAGRPGLAAPRYDLELLAPRLLGA
                     PATEVGLAPAPLPATAPSTGSRLFWAALVGAVVALLVLLARLLRRPSGPAGSEAGSGE
                     G"
     gene            7284..>7746
                     /locus_tag="P5164_10790"
     CDS             7284..>7746
                     /locus_tag="P5164_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44423.1"
                     /translation="MKGLPTARLLPPGDAPSPFGLHLVLGPLAGGAEEGPVPLPLSAD
                     EGTSRVYLGALKGDADSVVELVAVKLLPNVLPEALAESAARPTNRTLVARFAAERERM
                     RALRAESPYFPRLLQPDGGPEATLPALVYDKPSRTFFVPPCAACGRPLELLT"
BASE COUNT          737 a         3073 c         2784 g         1152 t
ORIGIN      
        1 cgtcccccct gccgcacgga tcaggaggcg gcccgcagcc tccctcccga ggcgttcgaa
       61 gaggcgccgc ggccagggcc cctcgagagg gagcgcggac tccccgagag cggcgtcgat
      121 cgccgcgtcg agcccggcga ggcgcgccgt gagcgcctcg ccggccctcg cgagccggac
      181 cgtcgctccc ggccagcggg cctcgagccc ggggaggagc tggcggcgga cgcggttccg
      241 ggcgaaccgt tcgtcggcgt tcgaggcgtc ctcgcgccag ggcaccccct cggagcgaag
      301 ccattcccgg agctcgccgc gccgaaaggg gaggagcggg cggacgacac cgtcctcccg
      361 gcggggccgg atgccacccc tcgagcggcc gagacgccgc tcgaggtgaa ggaggaccgt
      421 ctccgcctgg tcctcgagcg tgtggccggt cccgaggagc gtccccgggc cgaggtcccg
      481 cccgagcgcg agcagggatt catagcgaag gcgccgcgcg gccgactcga ggctctcccc
      541 cgtctcccgg aggtcccgga ccgggaagct ccggaagacg aaggacgcgc cgagcgacgc
      601 cgcgagatcg gccgcggcgc gcgcgtcccg gtgcccctcg acgccccgga gctggtggtc
      661 gacgtgaaag aggacgatcg ggacctcccg ccggcgggcc tccgcggaga ggaggagcgc
      721 gagggccgtg gagtcgctcc cgcccgagca ggcggcgacg aacgcggcgg cgccgtccag
      781 gaccccagac gaggcgacgt ggcgggcgaa ggcgtcgggc aggggctcgg agcggcccgg
      841 cgggacgggt ttctgcactc cgggagggta gccgacgcgg gccgggcggg gagaggtggt
      901 ggcgatgggc ggactcgaac cgccgacaca gggcttatga gacccccgct ctaccgactg
      961 agctacatcg ccacgagccg gccgcagagc cgcggatggt agcagatccc ggggatatga
     1021 tgtcgccatg cccttcctcg acgaagacga aggcgttcgc gccgaccagt gctcgcgttg
     1081 cgggcggatc tgcgagccgg ccgagatgga gacctgtccg gtctgcggca ggcggtactg
     1141 cagctactgc cggtacaggg tcggctctcg aagctactgc agccgtccct gcggcgactc
     1201 gttcttcttc ggcggcgacg tcgacgacga aggtctctcc gaggaatagc gacgggccgg
     1261 cgatgaggct cgcgtgacgg aggtcctcct cctcttcctg gggatggcgg tgctcctggc
     1321 gccgatcgtc tcgctcgtcg tctcgctcct cggccggagc cggctcacga aggccgaaga
     1381 gcggctcgga gagctcgaga ggtcgagaag cgcgctggag tcggagctcc gcgcgcttcg
     1441 cgtcgagatc ggaaagaggc gcgaaggggt ggcggaagcg ccgccgcccg cgcccgaagg
     1501 ggaggctcgg cccccggtgc cgccggccgc ggcggtttcc gcgccggtgc cggcaccgtc
     1561 ccggctcgag gacaccgacc ggatcacgcc gctgcccgtc ccgccgtacc ccgcgtctcc
     1621 gccgccacct cccgtcccgg cgcccgccgc ggtgccgccc cccgcgccga ctctcccggc
     1681 agcgcccccc gttccgccgg ccgcgtcggt tgcggttccg cccgttcctc cgaccttcgc
     1741 cgtgccgccg ctgccggccg ccgcgtcggc cggagcggcc tcgcggccgg cctccccagc
     1801 tgcggcgggt cgggcccccg cgccgacggt cccggctccc gcgccgccgc ggcccgcgcc
     1861 gccgcccgcc cggcccgcat ccgcccccgc ggcgcctccg aaagccccgt tcgactggga
     1921 gagcctcgtc ggcgtcaagc tcttctcctg ggtcgccgcg gtcgcgctcg tcgtcgcggc
     1981 ggtcctcttc ctccgctact cgatcgacca cggctggctg accccgccgc tccggatggc
     2041 gatcggcctc ctgaccggca tcggcctgct cgtcgcctgc gagctgaagg cggcgcggcg
     2101 ctacccggtc accgcgaacg cgctcgacgg agcggcgatc gccgtcctct tctccacgtt
     2161 cttcgcggcc catgcgctct ggaagctcgt cccggccctc ccgacgttcg gcctgatggc
     2221 gctcgtgacg ggagccgcgg tcctcctgtc ggtccgacgg gactcgatct tcatcgcgct
     2281 cctcggcctg gtgggcggct tcgcgacgcc ggccctcctc tcgacgggcg aggaccggcc
     2341 gttcggcctc ttcggctacc tcctcctcct gaacgccggg ctcgcctggg tggcgcaccg
     2401 gaagaagtgg cccgtcctga ccgtcctctg cgcgctcctc accgcgatct accagtgggg
     2461 ctgggtcgcg aagttcctga ccgaagggaa gctcggaatc gcggccgggg tcttcctcgt
     2521 gttccccctg gtcctctacg ccggcctcgc gctcgggaag cccgaggaga agggagcgga
     2581 cgggaaggcg ccgctcttcg agggggccgc gcgcctcgcc gggctcctgc cgctcctctt
     2641 cgcgctcttc ctcgcggcgg tgcccgccta cggggcgcgg cacggactcc tgttcgggtt
     2701 cctcttcctt ctcgccgcgg ggctcttcgc ggtcgcggtc tggcgcgggc cctcggcgct
     2761 ccacctggcc ggaggcctcg cgacgctcct cgtcttcgtc gtctgggcgt tccgcagcta
     2821 tcacccgggg gcctggccgg cgatcctcgc cttcctcggc ctcttcgtgg cgttcttcct
     2881 cgcggcgccg ttcgtggccg agaaggccgg gcgccccttc gatgcgcccg ggcgccgcgc
     2941 cgcgtacgcc gccccgctcc tcctctcgct ggcgccggtc ctcgtgtccg aggagccgcg
     3001 cgcggcctcg cccgcgctcc ttttcggcgt cgtcttcgtc ctcctcgcgc tctgcgccgc
     3061 gtacgcgatc cgccgcgagg aggggggcgt cttcttctcc ggagcgttct tcgcgctcct
     3121 cgcccaggcg gtctggtcgg cgaagcacct gacgccggag cggctcctgc cgggtctcgc
     3181 gctctacggc gtcttctcgc tcttctacct cggcgtcccg ctcctcgcgc gtcgtctccg
     3241 gaaggagctg aagcccggag gcctgtcggt cgtcctcctc ttcgcggctc tcgcgctcct
     3301 cctcttcctg gcggccgggc ccgtggcggc ctcggcgctc tgggggctcg cgctcctcct
     3361 cgccgttctg aacgtcggcc tgttcgcgga gggggccgcc ggacgctacc cggtcgcgac
     3421 gatcctcggc gtcctcctct cgtggggcgt cctggcggtc tggtggagat cggtccccgt
     3481 cgggacgctc ctcgtcccgg cgctcctcgt cgtcctcggc ttcgccctcg tcgtcctcgg
     3541 ggggagcgcg tgggcgcgtg cgaagaccgg ggccggagcg acgaccgggc cggacgggat
     3601 ccacctcggc ctcgtcgggc acctcttcct cctcttcgtc gccacgcagc cgcccctcgc
     3661 gtggccgccg gcgccgctcc tcggggttct cctcgtcctc gacctcgcgc tcgcggcggc
     3721 ggcgttctgg agcggcctcg gcgccctcct cgcggcggcg ctcgtcctgt cgcagatcgt
     3781 cctcctcgtc ttcgtcgccg cggtcccgcc ggcgcccacc gtggggctga tctcggcact
     3841 cggcgtcgcc gcgctcggcc tcctcgcgcg ggccctcgcg ccgcgggctc caaggggagg
     3901 cgaggggctc cgcctcgccg tcgcgcaggg ggcagccgcg gcgctcctcg tcgggcaggt
     3961 cgtcgcgctc ttcgccgccg gggcccgggg acggcccggc ctgccgctcc tcgtggccgc
     4021 cgtactcctc ctcgcggggg cgctcctggc ggtcgccgcg cgcgaagggt ggtacggcct
     4081 ctcggtcctc gcggcggccc tcgggttctt cacggtccac ctccaggcgg cggcgcagcc
     4141 cggcgcggac gtcgccgtgc cgcgcctcct cttcgccggc ctcgtctggc tcctcttcct
     4201 cgtccacgcg cccctcgcgg cacgccgggc gccctccgcc cgcgcgccgt gggtgacggc
     4261 ggtcctcgcg agcggcgcgt tcttcctcct cgcgcggcgg agcttctccg aggcgggctg
     4321 ggacgggttc gtcggagccc tcccggtcgc gatcgccgcc gcgctagcgc tcctcgtcct
     4381 ctcgctcctt aagatcgagg ctcccgccgc gcgcgacctc ggccggctcg ccctcgtcgc
     4441 cggcgccgcc ctcgcgttcg tgacggtcgc gattccgctc cagctcgaca aggaatggat
     4501 cacgatcggc tgggcgctcg aggcggcggc gctcgcgtgg ctctacacgc gcgtgccgca
     4561 ccgcgggctc ctcctcgcgt cgggcggcct cgcggcggtc gtcttcgtcc gactcgtcct
     4621 gaacgaggcg gtcctctcgt accacgcgcg gagcgcgacg ccgatctgga actggtacct
     4681 ctacacctac ctcgcggcgg ccgtcgcgct cttcgtcgcc gcgcgtttcc tccgggggac
     4741 cgccgacgac ctcctggacg gccggctccg cgtctcgacg gccgacgtcg tcttcgggac
     4801 ggtcctcctc ttcgcgctcg tcaacatcga gatcgcggac tggttcgcgg aaggccccac
     4861 gctcacgttc cacctcgcgc gcgggacgtc gctcgcgcag gacctcgcgt acacgctcgc
     4921 ctgggcggtc ttcggagtcg gcctcctcgc ggcgggcatc gtgggccgaa gccgggcggc
     4981 gcgcgccgcg gcgctcggcc tcctcgtcgt gaccgtcctg aaggcgttcc tccacgacct
     5041 cgggcggctc ggcggcctct accgcgtcgc ctcgttcgtc gggctggcga tctcgctctc
     5101 gctcgtcgcg atcgccatcc agaagttcgt cctccgcccc cgggagaagg catgaagccg
     5161 actcgcctcc tcttcgccgc cggcctcctc gccgccgccc tcccaggcgc ggcggaggag
     5221 ggggtccgac ccgtcccgtc tttccggcac gagcgggccg tccttcccgg cgctcccggc
     5281 ccgaaccgcc tcgacgtgga cgtttcgctc cttgccggct ccgaggcgcg gaccgcgggc
     5341 ggcctcctct ccgacctgcg tctccacgac gcggccgggc gggaagtgcc gcacctcctc
     5401 gtctcccccg ggcgcgccga gccggagtgg ctttccgcca ccctcctgcc gatcgcggcg
     5461 acgaagacgg cgagcggatt cgaggccgac ctcggtgcgc tccggcgcgt cgaccgcctc
     5521 cgcgtgagcg ggatcccggc gcccttcctg aagcgcctcc gtctcgaggg gagcggcgac
     5581 cgcgcgcgct gggcgctcct cgcggaggag gggacgctct tcgaccttcc cgaagagggg
     5641 ctctcgcgga cggtcgtcgc gttcgccgcg ggggagcacc ggtacttccg gctcgtctgg
     5701 aacgacgcga agagcggccg cgtcccgctg ccgtcgcgcg tcgaggcccg cgcggccggg
     5761 acgggcggcg cgccggaggc gctccgggcg gaggtcccgt tcgagcgacg ggagagcgag
     5821 ccggggagga gccggtatcg cctgcggctt ccggccgcgg ggctgccgat ctcggccctc
     5881 gaggtcggag tccgctccgg gaacgtcctg cgcgaggccc gcgtcctcga gtcgcgcctt
     5941 tcggccgggc ggctcgtccc gtacgagctg ggcgccgcga cgctccggag ggccgagcgc
     6001 gaggacgcgg cggcggccga gatgacgatc tccgtcgcgc cgcccgcgga ggccgagctg
     6061 gagctcgtcg tcgaggacgc ggggaacccg cccctcgagc tcgcgtcggt gacggccctc
     6121 ttcgaagggc tcccgttcgt ctacttcgag agcccggacg gcgcgcccct cgcggcccgg
     6181 tacggggcgc gggcgctcga gcggccgcgg tacgacctcg aggccgcgcg ttccgccgtc
     6241 acgaggcgcg gcgccgcccc gccggcgcgc gcggcgtggg gagaggcgcg cccgcggccg
     6301 gcgtccgccg cggaagcggc cgtggccccg tccgttccgg cgggcgcggc gctcgaccgc
     6361 acctcgttcc gcgtctcgcg agcgatcccg cccggtccgc ccggcctgac ggcgctccgt
     6421 ctcgacgcgg cggtcctcgc ccagagcccg agcctcgccg acgtccggat cgtctcctcc
     6481 gacgaccggc aggtccccta tctcctcgag acgctcgggg agccgctcgt cgtgaggctt
     6541 cccgccccgc agtcgaccgg cgacccgcgt ccgcgcgccg gagagggggc ggagggtcgt
     6601 ctctcgttcc accggatcga cctctcgtac gagacgctcc ccgcgacgcg tctcgagctc
     6661 gcgacgacgg cgcgggtctt cgcccggaac gtcgtcgtct accgcgaacg gctcggaggc
     6721 cggcccgaga cggccgggac gttcaccgcg atcgcctcct cggcgtggca gagcgccgac
     6781 ccggcgcgcg aggcgccgcg gctcgtcctc gacctgccgc cgggaacggg agggacgctc
     6841 ttcgtctcga tcgacgacgg tgacaacgcg cccctcccgc tcgcgggcgc gtcgcttctt
     6901 ctcccggcgc accgtctccg cttcgtccgg gaggacggcg cgctggcgct cctcgcgggc
     6961 cggccgggcc tcgccgcgcc ccgctacgac ctcgagctcc tcgcgccgcg gctcctcggg
     7021 gcgccggcga ccgaggtggg gctcgcgccg gcgccgctcc cggctacggc gccttccacc
     7081 gggtcgaggc tcttctgggc cgcgctcgtc ggggccgtcg tcgcgctcct cgtcctcctc
     7141 gcccgtctcc tgcggcggcc gtccgggccg gccgggagcg aggccgggag cggggaaggg
     7201 tagactcgcg tctccggagg aatttcagtg gtgagcgcgc gcgtgccgaa cgtccgtacg
     7261 gcgcggcggg cgggagggtc ccggtgaagg gccttcccac cgcccgcctc ctccctccgg
     7321 gcgacgcccc gtcgcccttc gggctccacc tcgtcctcgg accgctcgcg ggcggggcgg
     7381 aggaagggcc ggtcccgctg ccgctctcgg cggacgaagg gacgagccgc gtctacctcg
     7441 gagccctcaa gggggacgcc gacagcgtcg tggagctcgt cgccgtcaag ctcctgccga
     7501 acgtccttcc cgaggcgctc gccgagagcg ccgcgcgccc gacgaaccgg accctcgtcg
     7561 cgcgcttcgc agcggagagg gagcggatgc gcgcgctgcg cgccgagagt ccgtacttcc
     7621 cgaggctcct tcagccggac ggcggccccg aggcgacgct cccggccctc gtctacgaca
     7681 agccgtcgcg gacgttcttc gtcccgccgt gcgccgcgtg cggccggccg ctcgagctcc
     7741 tcaccg
//
LOCUS       DAOTEZ010000184         7720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_6692_length_7720_cov_6.719765, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000184 DAOTEZ010000000
VERSION     DAOTEZ010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7720
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6692_length_7720_cov_6.719765"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..883
                     /locus_tag="P5164_10795"
     CDS             77..883
                     /locus_tag="P5164_10795"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HRY44424.1"
                     /translation="MVVNEALRILRTVRYDAAHFSVFLQAGSIAAAAKPGQFVMIQAG
                     DGLRPYLRRAFSVADVTTVAGVPGIELVVRAVGVGTEALGRFAEGTPIPVLGPLGVPF
                     PIEDLKQSDRVALVAGGIGLAPLVLLARRLAERGIPADLFYGGRNESEVLKRPDFERF
                     LGAHRCRYATDDGSLGKKGRVTDIFGAALDAGTSYRRVFACGPVPMFRALAAILEARG
                     VEGSFALESEMACGFGVCLGCVLPGRDGRFHTICTEGPCLPPTAIDWSRV"
     gene            954..1898
                     /locus_tag="P5164_10800"
     CDS             954..1898
                     /locus_tag="P5164_10800"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HRY44425.1"
                     /translation="MSVDLTVHLGRLTLKNPIATASGTFGYGLEFAEFVDLSSLGAIS
                     VKGLSLRPCLGNPPPRICETDAGMLNAIGLQNVGIEEFLGEKLPRLHEARATVIANVW
                     GDTEAEYRAVVERIGDDPRVAAVELNVSCPNVVKGGMVFGNDPAALGALVAAVRPATR
                     LPLVVKLSPNVTDVVAAARAAVEGGADVLSLINTLVGLSIDIETREPKIGFTTGGLSG
                     PAIRPVAVRMVWEVRRALPHVPIFGIGGIETAEHAVEFLIAGANAVQVGTASFRDPGI
                     SGRLVRDLARWCERHGVERVSDLTATLKTRPRPEKAYV"
     gene            1891..2607
                     /gene="pyrF"
                     /locus_tag="P5164_10805"
     CDS             1891..2607
                     /gene="pyrF"
                     /locus_tag="P5164_10805"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRY44426.1"
                     /translation="MSDGRKRIAVALDASERSRILELARLVGPEIGVLKVGLEAFCAH
                     GPELVREVAELAPVFLDLKLHDIPNTVGGAAAAAARTGASILNVHAGGGLEMMRAAGE
                     RAREAAAAAGLPVPRVIAVTVLTSLDAPALSAVGLARSPREAALRLALLARQAGLDGV
                     VCSPEEIETIRSACGPEFLLVVPGIRPAGSAAGDQKRIATPGAAARAGADLLVIGRPI
                     TGAPDPAAAARAIAAEIGSA"
     gene            complement(2644..3054)
                     /locus_tag="P5164_10810"
     CDS             complement(2644..3054)
                     /locus_tag="P5164_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44427.1"
                     /translation="MAEGRRGRGRSAGDGLDADFGPGFEPAEEGSAEAASRSPRGPGA
                     EGASEPRWTAEEAGRIPCGRDGCLLKPIGDLAARFAFELARRGHTESAARAATSGIEL
                     LKALRDFLDEEIALAERSAEARRPAARYTKIPVE"
     gene            complement(3047..4252)
                     /locus_tag="P5164_10815"
     CDS             complement(3047..4252)
                     /locus_tag="P5164_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HRY44428.1"
                     /translation="MRILLYTGKGGVGKTTVAAATGLTLAARGLRTLVLSVDAAHSLA
                     DAFDLDGRLADKRRGAPMSVAENLWIQEVDVTEEIGRHWQDISGYVATLLAVTGIEEV
                     LAEELAVFPGMEEVSALLYVNRYAREGAYDVLILDCAPTGESLRFVSLPPTLDWYMKK
                     LFRVERSLLKVARPMAERVTSLPIPSERYFDNVKELAGKLDGVDALLKDPATTTVRLV
                     TNAEKIVLRETQRAFMYFGLYGLTVDAVIVNRLLPQGLADPFFGKWRRTQDRFLSEAR
                     GYFDPVPIWTVPLEDDQVVGAAGLAKLGAALYAGLDPAASFRTTPPYRFQKKPGHYVL
                     TLDLPFVQKEDVELAVASGDLIVTIGNFRHHVPIPRTLSGLVPTKARVEAGRLTVRFE
                     RGAAEAERG"
     gene            complement(4302..4721)
                     /locus_tag="P5164_10820"
     CDS             complement(4302..4721)
                     /locus_tag="P5164_10820"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HRY44429.1"
                     /translation="MNREKGRPEVEAGAGGVRLDVFLDVACIYPTRSQAKEACEGGKV
                     DLNGASAKPHRIVRPGDTLQVTVPGRRRTLVVKAVAERHVPKSEARTLYEETTPEPTP
                     EQREAQRLERLLAPRRDAGAGRLSRREREERGRLRGY"
     gene            complement(4764..5126)
                     /locus_tag="P5164_10825"
     CDS             complement(4764..5126)
                     /locus_tag="P5164_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44430.1"
                     /translation="MSSRTRTRGEGAFGTIVGIAIVVVVGIALFKIVPLHYAGNKVKD
                     AMSEQANFAGMKPPDKIQYEIFVIAQEAGTPLTIQDIKVRRHGSDVIVEAKYIQKVSV
                     LGYQYTYAFDHTVQKPVF"
     gene            complement(5159..5881)
                     /gene="lepB"
                     /locus_tag="P5164_10830"
     CDS             complement(5159..5881)
                     /gene="lepB"
                     /locus_tag="P5164_10830"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRY44431.1"
                     /translation="MAPAAGDGPAPRVPRHRTTLREYVEAALVAVVFAFFAKAFLVET
                     YQIPSGSMEESLLVGDHVVVDKLGWAPRGLPWGPLLPARGLRRGDVVVFRGPEEPGRD
                     FIKRAVALPGETLEIRGKRLFVDGAAQEEPWAVHRDPAVLSGEDLPASVRGRDELPLR
                     TLPPDAFFALGDNRDESRDSRAWGPVPLGHVRGRALFVYWSVRPSPESIVGRNAGLRR
                     LLDGAIHFFSRTRWERTFRAVD"
     gene            complement(5893..7692)
                     /gene="lepA"
                     /locus_tag="P5164_10835"
     CDS             complement(5893..7692)
                     /gene="lepA"
                     /locus_tag="P5164_10835"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRY44432.1"
                     /translation="MPDPSRIRNFSIVAHIDHGKTTLSDRILEITGALTAREMREQVL
                     DDNPIEQERGITIKARAVSLKYRSEDGQDWVLNLIDTPGHVDFTYEVSRSLAACEGAV
                     LVVDATQGVEAQTLANAYLAIHGDLTIVPCINKVDLPSAVPEMAREQIEEVIGIPAQH
                     AALTSGKVGTGVRDLLEHIVHDVPAPTGNAAGPLRALLFDSWFDVYRGVTCLVRVKDG
                     AIRPGMKIKMLGMDLVSEVQEVGVFTPKAQVVPELTVGMVGYVIAGIKDLHSTKIGDT
                     MTEAERPASEPLPGFQDVKPMVFASIFPTESDDYEDLRDAMEKLRLNDASFTFEPESS
                     TALGFGFRCGYLGLLHLEIVQERLEREFDLSLITTAPSVPYRVTTTRGEVLEVSNPVK
                     LPETQLIDVIEEPRILATIITKDEYLGGILALAEERRGRQVSLEYAGTQRAVLKYDLP
                     LNEVILDFYDKLKSVSRGYASFDYQLTGYAPADLVKLDVLINSEPVDALAFIVHRDKA
                     QVKGRALVEKMKEMIPQQMFEVIIQAAIGAKILARSTVKALRKNVLAKCYGGDITRKR
                     KLLEKQKEGKKRMKKVGRVELPQEAFLAVLKVD"
BASE COUNT         1023 a         2766 c         2750 g         1181 t
ORIGIN      
        1 gccccgagga gcgcggggtg gctgcgccgg agggcctcgc tcataatccg cgtccgcgag
       61 tctcgccgtc ttccgcatgg tggtcaacga agcgctccgc atcctccgca ccgtccgcta
      121 cgacgcggcc cacttctccg tcttcctgca ggccggctcg atcgcggctg ccgcgaagcc
      181 ggggcagttc gtcatgatcc aggccggcga cggcctccgg ccctacctcc gccgcgcgtt
      241 ctccgtcgcc gacgtgacga cggtggcggg cgtccccggc atcgagctcg tcgtgagggc
      301 cgtcggggtc gggacggagg cgctgggccg attcgcggag gggacgccga tcccggtcct
      361 cggaccgctc ggcgtgccgt tcccgatcga ggacctgaag cagtccgacc gggtggccct
      421 cgtggcgggc ggcatcggcc tcgcaccgct cgtccttctc gcgcggcgcc tcgccgagcg
      481 cgggataccg gccgacctct tctacggcgg ccggaacgag agcgaggtgc tgaagcgtcc
      541 cgacttcgag cgcttcctcg gcgcccaccg ctgccgctac gcgacggacg acggctcgct
      601 cgggaagaag gggcgcgtga ccgacatctt cggcgccgcc ctcgacgccg ggacgagcta
      661 ccgtcgggtc ttcgcctgcg gccccgtccc gatgttccgg gctctcgccg cgatcctcga
      721 ggcgcggggc gtggaggggt cgttcgcgct cgagtcggag atggcatgcg ggttcggcgt
      781 ctgcctcggc tgcgtcctcc cgggccgcga cgggcgcttc cacacgatct gcaccgaggg
      841 gccctgcctc ccgccgacgg cgatcgactg gagccgggta tgagcgtcga cctgacggtc
      901 ctgttcccgg gggtatcgcg ccccccccgg accccctacc ggagctgcga ccgatgagcg
      961 tcgacctcac cgtccacctc ggccgcctca cgctgaagaa cccgatcgcc accgcgtccg
     1021 gcaccttcgg gtacgggctg gagttcgcgg agttcgtcga cctctcgtcg ctcggagcga
     1081 tctcggtcaa gggtctctcg ctccggccct gcctcggcaa cccgccgccc cgcatctgcg
     1141 agaccgacgc cggcatgctc aacgcgatcg ggctccagaa cgtcggcatc gaggagttcc
     1201 tcggcgagaa gctcccgcgg ctccacgaag cgcgcgcgac cgtcatcgcg aatgtctggg
     1261 gcgacacgga ggcggagtac cgcgccgtcg tcgagaggat cggcgatgac ccgcgcgtcg
     1321 ccgccgtcga gctgaacgtc tcctgcccga acgtcgtcaa ggggggcatg gtcttcggga
     1381 acgaccccgc ggccctcggc gccctcgtcg ccgcagtccg ccccgcgacg cgcctccctc
     1441 tggtcgtcaa gctctcgccg aacgtcaccg acgtcgtcgc cgcggcgcgg gccgcggtcg
     1501 aaggcggggc cgacgtcctt tccctgatca acacgctcgt cggcctctcg atcgacatcg
     1561 agacgcgcga gccgaagatc ggcttcacga ccggcgggct ttccggcccg gcgatccgcc
     1621 cggtcgccgt ccggatggtc tgggaggtcc ggcgcgccct gccccacgtc ccgatcttcg
     1681 ggatcggcgg catcgagacc gccgagcacg ccgtcgagtt cctcatcgcc ggggcgaacg
     1741 ccgtccaggt cggcacggcg agcttccgcg acccggggat ctccggccgg ctcgtccgcg
     1801 acctggcgcg ctggtgcgag cggcacggcg tggagcgggt ctccgacctc actgcgacgc
     1861 tgaagacgcg cccgcgcccc gagaaggcgt atgtctgacg ggcggaagcg gatcgcagtc
     1921 gccctggacg cctccgagag gagccggatc ctcgagctgg cgcgcctcgt cggccccgag
     1981 atcggggtcc tgaaggtcgg cctcgaggcc ttctgcgcgc acggccccga gctcgtccgc
     2041 gaggtcgccg agctcgctcc cgtcttcctc gacctcaagc tccacgacat cccgaacacc
     2101 gtcggcgggg cggccgccgc ggccgcgcgg accggcgcgt cgatcctgaa cgtccacgcg
     2161 ggcggaggcc tcgagatgat gcgtgccgcc ggcgagcgtg cgcgggaagc cgcggccgcg
     2221 gcggggcttc cggtccccag ggtgatcgcg gtgacggtcc tgacgtcgct cgacgccccg
     2281 gcgctctcgg cggtcggact cgccaggagc ccgcgggagg ccgcccttcg gctcgccctc
     2341 ctcgcgcggc aggcgggtct cgacggcgtc gtctgctctc cggaggagat cgagaccatc
     2401 cgctcggcct gcggcccgga gttcctcctc gtcgttccgg gcatccggcc ggcggggagc
     2461 gcggcgggag accagaagcg gatcgccacg cccggcgcgg cggcgcgcgc gggggccgac
     2521 ctcttggtga tcggccggcc gatcacgggc gcgcccgacc cggccgcggc ggcgcgagcg
     2581 atcgccgcgg agatcggctc ggcctgacgc gcgggcgggc gctcggtcgc cgagggcgcg
     2641 cgcctactcg accgggatct tcgtgtagcg cgccgcggga cgccgggctt cggccgaccg
     2701 ctcggcgagc gcgatctcct cgtcgaggaa gtcgcggagc gctttcagca gctcgatccc
     2761 ggaggtcgct gcccgcgcgg ccgactcggt atggccgcgg cgcgccagct cgaacgcgaa
     2821 gcgggccgcg aggtcgccga tcggcttcag gaggcagccg tcccttccgc aggggatcct
     2881 cccggcctct tccgcggtcc accgcggctc gctcgcgcct tctgcgcccg gcccgcgggg
     2941 ggaccggctc gccgcctcgg cgctcccctc ctccgccggc tcgaacccgg gcccgaaatc
     3001 ggcatcgagg ccgtctcccg cgcttcgtcc ccgccccctg cgtccctcag ccacgctctg
     3061 cctccgccgc gccccgttcg aagcggaccg tcagccgccc cgcctccacc ctcgccttcg
     3121 tcgggacgag ccccgacagc gtccgcggga tcgggacgtg gtgccggaag ttcccgatcg
     3181 tcacgatcag atcgcccgag gcgaccgcga gctcgacgtc ctccttctgg acgaagggaa
     3241 ggtcgagcgt caggacgtag tgtcccggct tcttctggaa gcggtacggg ggcgtcgtcc
     3301 ggaacgacgc ggccggatcg agccccgcgt agagcgcggc gccgagcttc gccaggccgg
     3361 cggccccgac gacctggtcg tcctccagcg ggacggtcca gatcggcacg gggtcgaagt
     3421 agccccgggc ttccgagagg aaccggtcct gcgtccgccg ccacttcccg aagaacggat
     3481 ctgcgagccc ctgcgggagg aggcggttca cgatgacggc gtcgaccgtg aggccgtaga
     3541 gcccgaagta catgaaggcc cgttgcgtct cgcgcaggac gatcttctcg gcgttcgtca
     3601 cgaggcggac ggtcgtcgtc gcagggtcct tcagcagcgc gtcgacgccg tccagcttcc
     3661 cggcgagctc cttcacgttg tcgaagtacc gctcggacgg gatcgggagg ctcgtcaccc
     3721 gctcggccat gggccgggcg accttcagga gcgaccgctc gacccggaag agcttcttca
     3781 tgtaccagtc gagcgtcggc gggagagaga cgaaccggag cgactccccg gtcggcgcgc
     3841 agtcgaggat caggacgtcg taggcgccct cgcgggcgta ccgattcacg tagaggaggg
     3901 cggaaacctc ctccatgccg gggaagacgg cgagctcctc cgccaggacc tcctcgatgc
     3961 cggtgacggc gaggagcgtc gcgacgtagc cggagatgtc ctgccagtgc cggccgatct
     4021 cctcggtgac gtcgacctcc tggatccaga ggttctccgc gaccgacatc ggggcgccgc
     4081 gccgcttgtc ggcgaggcgg ccgtcgaggt cgaaggcgtc ggcgagggag tgcgccgcgt
     4141 ccaccgacag cacgagcgtc cggaggcccc gggcggccag cgtcaggccc gtcgctgcgg
     4201 cgacggtcgt cttgccgacc ccgcccttgc ccgtgtagag gaggattctc atcggagagg
     4261 aggggcatct tagcccgcgc cgacgcggcg gcggcgcggg gtcagtagcc gcggagccgg
     4321 ccgcgctcct cccgctcgcg ccgggagagc cggccggccc cggcgtcacg gcgcggggcg
     4381 aggagcctct cgagccgctg cgcctcccgc tgctccggcg tgggctcggg ggtcgtctcc
     4441 tcgtagagcg tccgcgcctc ggacttcggg acgtgccgtt cggccaccgc cttcacgacg
     4501 agggtcctcc gccgccccgg gacggtgacc tggagggtgt cgcccggcct gacgatccgg
     4561 tggggcttcg ccgacgcgcc gttcaggtcg accttccccc cctcgcacgc ctccttggcc
     4621 tgcgagcggg tcgggtagat gcaggcgacg tcgaggaaga cgtcgaggcg gacgcctccg
     4681 gcgcccgcct cgacctccgg acggcccttt tcccggttca tcctcgaacc gccgtgcggc
     4741 taggccgccg ccccgcgttc acttcagaag accggcttct ggacggtgtg atcgaacgcg
     4801 taggtgtact ggtagccgag gacggagacc ttctggatgt acttcgcttc gacgatcacg
     4861 tcgctgccgt gacgccggac cttgatgtcc tggatcgtga ggggcgtccc cgcctcctgg
     4921 gcgatgacga agatctcgta ctgaatcttg tcgggcggct tcattcccgc gaagttggcc
     4981 tgctcgctca tcgcgtcctt caccttgttg ccggcatagt gcagcggcac gatcttgaag
     5041 agcgcgattc cgacgacgac gacgatcgcg attccgacga tcgtgccgaa ggccccctcg
     5101 ccgcgagtcc gtgtccggga cgacatgctc tgcctcctcc ttgggccttc cgcgtcgctc
     5161 agtcgacggc tcggaaggtc cgctcccagc gggttctcga gaaaaagtgt atcgcgccgt
     5221 cgaggagacg gcgcagcccc gcgttccggc ccacgatcga ctccggggaa ggccggacgg
     5281 accagtagac gaacagggcc cgcccccgga cgtgccccaa cggcacgggc ccccaggcgc
     5341 gcgagtcccg ggactcgtcg cggttgtcgc ccagcgcgaa gaaggcgtcc ggcgggagcg
     5401 tccggagcgg cagctcgtcc ctcccgcgga cggaggcggg gaggtcctct ccggaaagga
     5461 cggcagggtc ccggtgcacg gcccagggct cctcctgggc ggcgccgtcg acgaagagcc
     5521 gcttcccgcg gatctcgagc gtctcgcccg gcagggcgac cgcccgcttg atgaagtccc
     5581 gcccgggctc ttccgggccg cggaagacga cgacgtcgcc ccgccggagc ccgcgcgccg
     5641 gcaggagggg cccccagggg aggccgcgcg gcgcccagcc gagcttgtcg acgacgacgt
     5701 ggtcgccgac gaggaggctc tcctccatcg acccgctcgg gatctggtag gtctcgacga
     5761 ggaacgcctt cgcgaagaac gcgaagacga ccgccacgag ggccgcctcg acgtactcgc
     5821 gcagggtggt gcggtggcgc gggacgcggg gcgccgggcc gtccccggcg gcgggggcca
     5881 cgctccgccc cctcagtcga ccttcaggac cgcgaggaag gcttcctgcg ggagctcgac
     5941 ccggccgacc ttcttcatcc gcttcttgcc ttccttctgc ttctcgagga gcttcctctt
     6001 ccgcgtgatg tcgccgccgt agcacttcgc gaggacgttc ttgcggagcg ccttcaccgt
     6061 cgagcgggcc aggatcttcg ccccgatcgc cgcctggatg atcacctcga acatctgctg
     6121 cgggatcatc tccttcatct tctcgacgag ggcgcggcct ttgacctgcg ccttgtcgcg
     6181 gtggacgatg aaggcgagcg cgtcgaccgg ctcggagttg atgaggacgt cgagcttgac
     6241 gaggtcggcc ggtgcgtacc cggtcagctg gtagtcgaac gaggcgtagc cgcgcgagac
     6301 cgacttgagc ttgtcgtaga agtcgaggat gacctcgttg agcgggaggt cgtatttcag
     6361 gacggcgcgc tgcgtgccgg cgtactcgag ggagacctgc cggccgcgcc gctcctcggc
     6421 gagcgcgagg atgccgccga ggtactcgtc cttcgtgatg atcgtcgcga gaatccgcgg
     6481 ctcctcgatc acgtcgatga gctgcgtctc ggggagcttg acggggttcg agacctcgag
     6541 gacctcgccg cgcgtcgtcg tgacgcggta ggggaccgag ggggcggtcg tgatgagcga
     6601 gaggtcgaac tcgcgctcga gccgctcctg gacgatctcg aggtggagga ggccgaggta
     6661 gccgcagcgg aacccgaacc cgagcgccgt cgacgactcg ggctcgaacg tgaacgacgc
     6721 gtcgttcagc cggagcttct ccatcgcgtc gcggaggtcc tcgtagtcgt ccgactcggt
     6781 cgggaagatc gaggcgaaga ccatcggctt gacgtcctgg aagccgggaa gcggctcgga
     6841 cgccgggcgc tcggcctcgg tcatggtgtc gccgatcttc gtggagtgca ggtctttgat
     6901 gccggcgatg acgtacccga ccatcccgac cgtcagctcg gggacgacct gggctttcgg
     6961 cgtgaagacg ccgacctcct gcacctcgga gacgaggtcc atcccgagca tcttgatctt
     7021 catcccgggc cggatcgcgc cgtccttgac gcggacgagg caggtgacgc cccggtagac
     7081 gtcgaaccac gagtcgaaga ggagggcacg gagcggaccg gcggcgttcc ccgtcggcgc
     7141 cgggacgtcg tggacgatgt gctcgaggag gtcgcggacc ccggtgccga ccttccccga
     7201 ggtgagcgcg gcgtgctgcg ccgggatccc gatgacctcc tcgatctgct cacgggccat
     7261 ctcggggacc gcgctcggga gatcgacctt gttgatgcac gggacgatcg tcaggtcccc
     7321 gtggatcgcg aggtaagcgt tcgcgagcgt ctgcgcctcg accccctgcg tcgcgtcgac
     7381 cacgaggacc gccccctcgc acgccgcgag ggagcgggag acctcgtagg tgaaatcgac
     7441 gtggcccggc gtgtcgatca ggttcaggac ccagtcctgg ccgtcctcgc tccggtactt
     7501 cagcgagacc gctcgggcct tgatcgtgat gccgcgctcc tgctcgatcg ggttgtcgtc
     7561 gaggacctgc tcgcgcatct cgcgcgccgt cagggcgccg gtgatctcga ggatccggtc
     7621 ggagagcgtc gtcttgccgt ggtcgatgtg ggcgacgatc gagaaatttc ggatgcggga
     7681 gggatcgggc atggtgtgag tcgggcctcg cgcccgaacc
//
LOCUS       DAOTEZ010000185         7715 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_6697_length_7715_cov_6.201697, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000185 DAOTEZ010000000
VERSION     DAOTEZ010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6697_length_7715_cov_6.201697"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="P5164_10840"
     CDS             complement(<1..719)
                     /locus_tag="P5164_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006460057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY44433.1"
                     /translation="MSSPPDTDGAPLEGRENSYGTESIKLLKGLEAVRKRPGMYIGNT
                     DDVTGLHHMVTELVDNAIDEAQVGYCDRVTVTVHADETVTVEDNGRGIPVGIHPVHGR
                     ETLEIILTDLHSGGKFDDNAYKVSGGLHGVGLSVVNALSSSLLVEVRREGRVWQQPYA
                     KGKPQQPIREVGRTNKTGTKLTFQADPEIFSVRSYSFDALSQRLRELAFLNPGVTIEV
                     VDERDEKGERKHLFRYEGGISS"
     gene            complement(703..1875)
                     /gene="recF"
                     /locus_tag="P5164_10845"
     CDS             complement(703..1875)
                     /gene="recF"
                     /locus_tag="P5164_10845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HRY44434.1"
                     /translation="MIGTLSVESFRNLAPARLRFHPRLNLLVGKNGQGKTNVLESLAL
                     VSGRASFRTADLADVVREGENDATVTVGLAGEPAGELAARIGKGRREHFWNGRKVSRV
                     EASRRLPLVALTSADLGRLGGPPADRRRALDRVAVAHEPSLAAEHRRYEKARADRVAL
                     LGAPGRPDRDALAAYEELLSSAGAAIAASRRRWAGPLGRRIEENARLLGLPYREISLR
                     LVSELPAEAPLPLLADALRAGLRLREESERRAGRALFGPHRDDLLLFSGALPLAARAS
                     SGEARALVLAWALAERSLLAEACGRLPVFAFDDFDSEWDPDVLARFARALPDDGQVFL
                     TSARERAARELPLPVGAVWRVEAGRLAATGTSPDRAAFLPAPARWPGERSDDVLAS"
     gene            complement(1897..3021)
                     /gene="dnaN"
                     /locus_tag="P5164_10850"
     CDS             complement(1897..3021)
                     /gene="dnaN"
                     /locus_tag="P5164_10850"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRY44435.1"
                     /translation="MELTLPAATLAREMSLMQGVVERRTPHQILSNVLLEARDGMLTL
                     TATDLDTTFVSEIEPLSLAVPGAVTVPARKLFDVVRDLPPAAAVRLRVLENHHVQVDS
                     KDPKFRIRLNGLAATDFPTRPEAAGGPTLTLPFGALRRMVEKVRFAVSTEEIRLQLQG
                     ALLKPKGTGLEMAATDGHRLALIEVEDAKADAGFEAILVSKKVLDELSRLDAEDETPV
                     TIIRGTNHIGFTLGRRRIFSKLDDRRFPDYEKVISKENTKRATVVREELLGAVRRISL
                     LSGDRLKAVSLSFKEGALVVSSESQDVGDGDQEIGAEYDADPVTLRLNSRYLEQFLEA
                     CETEKVHLFVKDEGSQCQGRPAEPVPGLKSYLYVVMPMRV"
     gene            complement(3244..4560)
                     /gene="dnaA"
                     /locus_tag="P5164_10855"
     CDS             complement(3244..4560)
                     /gene="dnaA"
                     /locus_tag="P5164_10855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRY44436.1"
                     /translation="MEKPTSWEKILEHLKSRVDERDFDTWFRGTRQKGETTDAVFVLI
                     SSPLFIDYIPREYGDQIAEAARLAGLGAREIRFVLEGRDDARAVVSPARPQPNRSRVG
                     DLNPAYTFEHFVIGKPNQLAHAAALHVADQTSHRYNPLFICGPTGVGKTHLMQAIGHR
                     RLSRRPGERVLYVYSETFVKEVVTGIRFNRMDQVRQRLLNADVLLFDDVQFLAGKATT
                     EDELFQTFNALYSIGSQIVFTSDVLPRDIPGLTDRMKSRFEGGLVVDIQQPDFETKVA
                     ILRHKAEIARVELDDDTAYYLSGKIKTHVRELESYFNRVVLVSSMRGEPITRDLAEEA
                     LRGILPDDSSKTSPAEILKAVAAHYGLKPAELRARTKREKIVFPRQVAMYVLKEAIGL
                     SLPEIGRLFGDKHHTTALHSIKKISARREEDPDFDREVAVLVAQFR"
     gene            4734..4868
                     /gene="rpmH"
                     /locus_tag="P5164_10860"
     CDS             4734..4868
                     /gene="rpmH"
                     /locus_tag="P5164_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRY44437.1"
                     /translation="MKRTFQPNNRRRKKTHGFLVRMRTKNGRAVLARRRAKGRKRLTV
                     "
     gene            4913..5308
                     /gene="rnpA"
                     /locus_tag="P5164_10865"
     CDS             4913..5308
                     /gene="rnpA"
                     /locus_tag="P5164_10865"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRY44438.1"
                     /translation="MSPDPARAPSGSSRTRGPESFGKDRRILRRAEYLQVYENGRRLA
                     GRKLVFFLRPSSCDRTRLGITITKKTGSSVVRNRLRRRLRELFRRTEAFSVPVDLVVN
                     VRPGAEMAPYHDLAEDFARLVRRAEEPTR"
     gene            5305..5538
                     /gene="yidD"
                     /locus_tag="P5164_10870"
     CDS             5305..5538
                     /gene="yidD"
                     /locus_tag="P5164_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020217597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRY44439.1"
                     /translation="MSASGPGPGARAAAGLLSAYKRYVSPLLPPACRFLPTCSEYARE
                     AILRHGLPRGTWLTIRRLARCHPFHPGGFDPVP"
     gene            5560..7260
                     /gene="yidC"
                     /locus_tag="P5164_10875"
     CDS             5560..7260
                     /gene="yidC"
                     /locus_tag="P5164_10875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRY44440.1"
                     /translation="MDSKRLALAFGASAAILILWTLLFPPPKPAPRAATPAMPTKATG
                     AGEGAANPAATPMPTAAAAPAAGPAPAAVAPIVAEAPRHLTVKNGVLAVRLSNRGGEV
                     VSAGLIRFRGGHGEDVPLELVRKDAPFAGRTLVYDPADPFLARAGAALHETTVETGRN
                     EVVVRFRYREADGQGVTRTYTFGTGYVVGLRVEREGGRNGTAAVVLGPGVGNPTEEEL
                     ANRYTKPGSSVTLSGSRSVSRKAKDGLKEAVALGSGFLAAGLEDNYFVTAFLPGAKET
                     VTLRPVSLVAAAGPAKGPVPAAAPEPLVETEVVVAAAGTLEAGLYLGPKDIDVLSTVR
                     PGMEKLIDYGWFAILVSPLLWLLKQFHAAIPNWGVAIILATVVIKVVLFPLTWKQLVS
                     MKKMSALQPKMEAIRQKWTPKLKSDPQARLKMNEETMALYKAEKVNPAGGCLPLLLQM
                     PILIAFYNLLSHAIDLRHAPFALWIQDLSAKDPYYVTPILMTLTMWLQQQMMPATGDA
                     TTRKVMGFLPLVFGFMFKDMPSGLVLYWLVQNVLSIAQQLLLERYTDLGMRPKAAKKA
                     "
     gene            7298..>7715
                     /locus_tag="P5164_10880"
     CDS             7298..>7715
                     /locus_tag="P5164_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020008859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44441.1"
                     /translation="MALLVADGEGTADVLVARATPPGAGALSIVRLSGSPGAALSIAR
                     AIAPRLPARPEPRRAYLSLFRDETGALLDEGLALYFAAPRSATGEEVVELQSHGAPAI
                     AEGLVAAAVALGARRARPGEFTRRALANGRVDLARAE"
BASE COUNT         1130 a         2573 c         2839 g         1173 t
ORIGIN      
        1 gaggagattc ctccctcgta gcggaagagg tgcttgcgct ctcccttctc gtccctctcg
       61 tcgacgacct cgatcgtgac gccggggttg aggaaggcga gctcgcgcag gcgctgcgac
      121 agggcgtcga agctgtagga gcggaccgag aagatctccg ggtccgcctg gaacgtgagc
      181 ttcgttcccg tcttgttcgt ccggcccact tcccggatcg gctgctgcgg ctttcccttc
      241 gcgtagggct gctgccagac gcgaccctcc cggcggacct cgaccaggag ggaggaggag
      301 agggcgttca ccacggagag gccgacgccg tggaggccgc cggagacctt gtaggcgttg
      361 tcgtcgaact tcccgccgga gtggaggtcc gtgaggatga tctcgagcgt ctcgcggccg
      421 tggaccgggt ggatgccgac cgggatgccg cggccgttgt cctcgacggt cacggtctcg
      481 tcggcgtgca ccgtcacggt cacgcggtcg cagtagccga cctgcgcttc gtcgatcgcg
      541 ttgtcgacga gctcggtcac catgtggtgg agccccgtga cgtcgtcggt gttcccgatg
      601 tacatcccgg ggcgcttccg caccgcctcg agccccttca ggagcttgat cgactcggtc
      661 ccgtagctgt tttcgcggcc ctcgaggggg gcgccatccg tgtcaggagg cgaggacatc
      721 atcggacctt tcccccggcc accgggccgg ggcggggagg aacgccgccc ggtcgggcga
      781 ggtcccggtc gcggcgaggc ggccggcctc gacgcgccag acggcgccca ccggcagcgg
      841 gagttcgcgc gccgcgcgct cgcgcgcgga cgtgaggaag acctggccgt cgtcgggaag
      901 cgcccgcgcg aagcgcgcga ggacgtccgg gtcccactcg gagtcgaagt cgtcgaacgc
      961 gaagacgggg aggcgcccgc aggcctcggc gaggaggctc cgctccgcga gcgcccacgc
     1021 gaggacgagg gcccgcgcct ccccggagga agcccgcgcc gcgagcggga gcgcgccgga
     1081 gaagaggaga aggtcgtctc ggtgcggtcc gaagagcgct ctccccgcgc gccgctcgct
     1141 ctcctcccgg agccggagtc ccgcgcggag cgcgtcggcg aggaggggga gcggggcctc
     1201 cgcgggaagc tccgagacca gccggaggga gatctcccgg tacgggaggc cgaggaggcg
     1261 ggcgttctcc tcgatcctcc ggccgagcgg gccggcccag cgccgccgcg aggccgcgat
     1321 cgcggctccg gcggaggaga ggagctcctc gtacgcggcg agggcgtccc ggtcgggccg
     1381 accgggcgcg ccgaggagcg cgacgcggtc ggcgcgcgcc ttctcgtaac gtcggtgctc
     1441 ggccgcgagg gagggttcgt gggcgaccgc cacgcggtcg agcgcacggc gtcgatcggc
     1501 gggcggcccc cccagccggc ccaggtcggc ggacgtcagc gcgacgaggg ggagccggcg
     1561 gctggcctcc acgcgcgaga ctttccggcc gttccagaag tgctcccggc gtcccttccc
     1621 gatgcgggcc gcgagctcgc cggccggctc tcccgcgaga ccgacggtca ccgtcgcgtc
     1681 gttctctccc tcgcggacga cgtccgcgag gtcggcggtc cggaaggacg cgcgcccgga
     1741 cacgagagcg aggctctcga gcacgttcgt cttaccctgt ccgttcttgc cgacgaggag
     1801 gttcagccgc gggtgaaatc tgagacgggc gggcgcgagg ttacgaaagg actcgaccga
     1861 gagggttccg atcaccacgt gtcgacgcgc accccctcag acgcgcatcg gcatgacgac
     1921 gtagaggtag ctcttcagcc cgggcacggg ctccgcaggc cttccctggc actgcgaccc
     1981 ctcgtccttg acgaagaggt ggaccttctc ggtctcgcac gcctcgagga actgctcgag
     2041 gtagcgcgag ttcagccgga gggtgacggg gtcggcgtcg tactcggcac cgatctcctg
     2101 gtcgccgtcg ccgacgtcct ggctctcgga ggagacgacg agggcccctt ccttgaagga
     2161 aaggctgacg gccttcagcc ggtcgccgga gaggagcgag atccggcgga cggcgccgag
     2221 cagctcttcc ctcacgacgg tcgcccgctt cgtgttttcc ttgctgatga ccttctcgta
     2281 gtcggggaag cgccggtcgt cgagcttgga gaagatgcgc cgccgcccga gcgtgaaccc
     2341 gatgtggttc gtcccgcgga tgatcgtgac gggcgtttcg tcctcggcgt cgaggcgcga
     2401 gagctcgtcg aggaccttct tcgagacgag gatcgcctcg aacccggcgt cggccttggc
     2461 gtcctccacc tcgatcaggg ccagtcggtg cccgtcggtc gcggccatct cgaggcccgt
     2521 ccccttcggc ttcaggagcg ccccctggag ctggagacgg atctcttccg tcgagacggc
     2581 gaaccggacc ttctcgacca tccggcgcag ggccccgaag gggagcgtca gggtcggtcc
     2641 gccggccgcc tccgggcgcg tcggaaagtc cgtcgccgcc aggccgttga gacggatgcg
     2701 gaatttcgga tccttgctgt cgacctggac gtgatggttc tccagaaccc ggagacggac
     2761 tgccgcggcc ggcggcaggt cgcgtacgac gtcgaagagc ttccgggccg gaacggtgac
     2821 ggcgcccgga acggcgagcg agaggggctc tatctccgac acgaaggtcg tatcgaggtc
     2881 cgtcgccgtc agcgtgagca tcccgtcgcg ggcctcgaga aggacgttcg aaaggatctg
     2941 gtggggcgtc cgccgctcca ccacaccctg catcaggctc atctctcggg ccagggtggc
     3001 tgccggaagt gtcagctcca catcccctcc actgattcct taaattctag caccgaaaga
     3061 gttacagaag aagtagtagg gagggccgag ggctgtggga agaggccgga agggaagacc
     3121 ggtaccgggg ttagcgggac ggaagggatg tgcagatctc ggcggggccc tgtgcgtttc
     3181 gcgagtccga agccgggaac gaggagcggg ctccggagat tccacaggga ctcccgtgga
     3241 gattcaccgg aattgcgcga cgaggacggc gacctcccga tcgaaatcgg gatcctcctc
     3301 ccggcgggcg gagatcttct tgatcgagtg aagggccgtc gtgtggtgct tgtcgccgaa
     3361 aaggcgaccg atctcgggaa gggagaggcc gatcgcctcc ttgaggacgt acatcgcgac
     3421 ctggcgcggg aagacgatct tctcccgctt cgtccgggcc cgcagctcgg cgggcttcag
     3481 gccgtaatgg gcggcgacgg ccttgaggat ctcggcgggc gacgtcttcg aggagtcgtc
     3541 ggggaggatt ccccgaaggg cctcctccgc gaggtctcgg gtgatcggct cgccccgcat
     3601 ggaggagacg aggacgaccc ggttgaaata gctctcgagc tcgcggacgt gagtcttgat
     3661 cttccccgag aggtagtagg ccgtgtcgtc gtccagctcc acccgggcga tctcggcctt
     3721 gtgacgcagg atcgcgacct tcgtctcgaa gtcaggctgc tggatgtcga cgaccagacc
     3781 cccttcgaat cggctcttca tccgatcggt caggcccggg atgtcgcgcg ggaggacgtc
     3841 cgaggtgaag acgatctggc tcccgatgga atagagcgcg ttgaacgtct ggaagagctc
     3901 gtcctcggtc gtcgctttcc cggcgaggaa ctggacgtcg tcgaacagga ggacgtcggc
     3961 gttgaggagg cgctgccgga cctggtccat cctgttgaag cggatgccgg tgacgacctc
     4021 cttgacgaag gtctcggagt agacgtagag gacccgctcg ccgggacgcc gcgagaggcg
     4081 gcggtgaccg atcgcctgca tcaggtgggt cttcccgaca ccggtcggac cgcagatgaa
     4141 gagggggttg taccggtggg aagtctggtc ggcgacatgg agcgccgccg cgtgggcgag
     4201 ctggttcggc ttcccgatga cgaagtgctc gaacgtgtag gcggggttca ggtcaccgac
     4261 gcgggagcga ttcggctggg gccgggccgg ggagacgacg gcgcgggcgt cgtcccgccc
     4321 ctcgaggacg aaacggatct cgcgggcgcc gaggccggcg aggcgggcgg cctcggcgat
     4381 ctggtccccg tactcgcgcg ggatgtagtc gatgaagagg gggctcgaga tgaggacgaa
     4441 gacggcgtcc gtcgtctcgc ccttctgcct cgtgccgcgg aaccaggtgt cgaagtcgcg
     4501 ctcgtcgacc cggctcttca ggtgctcgag gatcttttcc caggaagtag gtttttccac
     4561 gacgactgca ccgacgcgag ggagaccgcc cggcaggagc gggggcggat gctagcatgc
     4621 ggcgaccgcg gacccggagt cgcggagccg ggcgggccgt ccacggttga caggaggccc
     4681 ggttctccgg agaatccgcc cctcgcgctc ccagattcgg gagaggtggt gccatgaaga
     4741 gaacgtttca gcccaacaac cgccgccgga agaagacgca cggctttctc gtccggatga
     4801 ggacgaagaa cggccgcgcg gtgctcgccc gtcggcgggc gaagggtcgc aagcgcctca
     4861 ccgtctgagg cttcccgggc ctccggccgg ttccggcgga gcttcggagt cgatgtcgcc
     4921 cgaccccgcc cgcgcccctt ccgggagctc ccggacgcgg ggcccggaat cgttcggaaa
     4981 ggaccgccgg atcctccggc gcgccgaata cctccaggtc tacgagaacg gccggcgtct
     5041 tgccggccgg aagctcgtct tcttcctgcg cccctcttcg tgcgatcgca cgcgcctcgg
     5101 gatcacgatc acgaagaaga ccggatcctc ggtcgttcga aaccggctcc gccgcaggct
     5161 tcgtgagctg ttccggcgaa cggaggcgtt ctccgttccc gtcgacctcg tggtgaacgt
     5221 gcggcccggg gcggagatgg cgccgtacca cgacctcgcc gaggacttcg cacggctcgt
     5281 ccggagggcg gaggagccga cgcgatgagc gcgtcgggcc ccggaccggg ggcgcgcgcg
     5341 gcggcgggac tcctttcggc gtacaagcgg tacgtctccc ctctccttcc ccccgcgtgc
     5401 cgattcctgc ccacctgctc ggagtacgcg cgggaggcga tcctccggca cgggctcccg
     5461 cgggggacgt ggcttacgat ccgccggctg gccaggtgcc acccgtttca cccgggcggg
     5521 ttcgacccgg tcccctgatc ccctctcgag gagttgacct tggactcgaa gcgtctcgcc
     5581 ctggcattcg gcgcctccgc cgccatcctg atcctctgga ccctgctctt cccgccgccg
     5641 aagccggcgc cgcgggcggc gacgccggcg atgccgacga aggcgacggg ggcgggcgag
     5701 ggcgcggcaa accccgccgc gacaccgatg cccaccgcag ccgcagcccc ggcggcgggc
     5761 cccgcgccgg ccgcggtggc gccgatcgtg gcggaggccc cgcgtcacct gacggtgaag
     5821 aacggcgtcc tggcggttcg gctgtcgaac cgtggcggcg aggtcgtctc cgcgggcctg
     5881 atccggttcc gcggagggca tggcgaggac gtgccgctcg agctcgtccg gaaggatgcc
     5941 cccttcgcgg gccggacgct ggtctacgac ccggccgacc ccttcctggc gcgagccggg
     6001 gccgcgctcc acgagacgac ggtcgagacc gggcggaacg aggtcgtcgt ccggttccgg
     6061 taccgggagg cggacgggca gggggtgacg cggacctaca cgttcggcac gggctacgtg
     6121 gtgggtctcc gggtggagcg cgaaggcgga aggaacggga cggcggccgt cgtcctcggt
     6181 ccgggcgtcg gcaatcccac cgaggaggag ctcgcgaacc ggtacacgaa gcccggctcg
     6241 agcgtgaccc tctcggggag ccggtcggtc tcgaggaagg ccaaggacgg gctaaaggag
     6301 gcggtcgcgc tcggttccgg gttcctcgcg gcggggctcg aggacaacta cttcgtgacc
     6361 gcctttcttc ccggcgcgaa ggaaacggtg acgctgcgtc cggtctccct cgtggccgcg
     6421 gccgggccgg cgaagggccc ggttccggcg gcggcgccag agccgctggt cgagaccgaa
     6481 gtggtggtcg cggcagccgg gacgctcgag gccggcctct acctcgggcc gaaggacatc
     6541 gacgtcctct ccacggtccg gcccgggatg gagaagctga tcgactacgg gtggttcgcg
     6601 atcctggtct cgccgctcct ctggcttctg aagcagttcc acgccgcgat cccgaactgg
     6661 ggggtcgcga tcatcctggc aacggtcgtc atcaaggtgg tcctcttccc gctgacctgg
     6721 aaacagctcg tctcgatgaa gaagatgagc gccctccagc cgaagatgga ggcgatccgg
     6781 cagaagtgga cgccgaagct gaagagcgat ccgcaggcgc gcctgaagat gaacgaggag
     6841 acgatggcgc tctacaaggc ggagaaggtg aaccccgccg gcggctgtct cccgctcctc
     6901 ctgcagatgc cgatcctgat cgcgttctac aacctcctca gccacgcgat cgaccttcgg
     6961 cacgcgccgt tcgcgctctg gatccaggac ctctccgcga aggacccgta ctacgtcacc
     7021 ccgatcctga tgacgctgac gatgtggctg cagcagcaga tgatgccggc cacgggcgac
     7081 gcgacgacga ggaaggtgat ggggttcctc ccgctcgtct tcgggttcat gttcaaggac
     7141 atgccctcgg gcctggtcct ctactggctc gtgcagaacg tcctgtccat cgcccagcag
     7201 ctcttgctcg agcgctacac ggacctcggg atgcgtccga aggcggcgaa gaaggcgtga
     7261 cgcgcgcgga cgtccgcccg gcggaggggc caaaacggtg gccctgctcg tcgccgacgg
     7321 cgaggggacg gcggacgtcc tggtggcccg ggcgacgccg cccggggccg gcgccctctc
     7381 gatcgtccgg ctgagcggtt cgcccggcgc ggcgctctcc atcgcgcggg cgatcgcgcc
     7441 ccggctgccg gcccggccgg agccgcggcg ggcctatctc tccctcttcc gggacgagac
     7501 gggcgctctc ctcgacgagg ggctggcgct ctacttcgcc gcgccgcggt cggcgacggg
     7561 ggaagaggtc gtcgagctcc agtcccacgg cgcgccggcg atcgccgagg ggctcgtggc
     7621 cgctgcggtg gcgctcgggg cacggcgcgc ccggccgggc gagttcacgc gccgggccct
     7681 ggcgaacggg cgggtcgacc tcgcgagggc ggagg
//
LOCUS       DAOTEZ010000186         7707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_6710_length_7707_cov_6.360429, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000186 DAOTEZ010000000
VERSION     DAOTEZ010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7707
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6710_length_7707_cov_6.360429"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="P5164_10885"
     CDS             complement(<1..751)
                     /locus_tag="P5164_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRY44442.1"
                     /translation="MYHSDTPKPGRRFLVPLLLAALLAAPACRKLRPAASEAEPRPVA
                     ARGDLAADERATIDLFRQSSPSVVFITTLSRRRTGLFRIAEIPRGEGSGFVWDTDGHV
                     VTNFHVIQGASSARVTLADGSTWPASFVGGAPDRDLAVLRIFPTGRTLRPILVGTSKD
                     LLVGQKVFAIGNPFGLDQSLSTGIVSALGRSIESVTGRQIEGVVQTDAAINPGNSGGP
                     LLDSAGRLIGVNTAIASTSGSSAGIGFAVPVD"
     gene            868..>2047
                     /locus_tag="P5164_10890"
     CDS             868..>2047
                     /locus_tag="P5164_10890"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRY44443.1"
                     /translation="MTAEPSSSLPPGALVGRYRILASVGSGAMGEVYRAEDTSLGRIV
                     ALKTLPLDLVGTPDRRVRFGNEGRVMASLSHPNLVQVFDVGEFEGTPYLVQEFVEGET
                     LEAILLGGAVPAPKAARWIAQAAEGLAAVHDAGFLHRDIKPSNLMVDRQGRIRVLDFG
                     LAKVQSVPANERKSQPSLTTDGFVVGTAQYMSPEQASGKKADARSDVFSLGAVFYELL
                     SGSSPFGGETAVDVMYAILHLPTPPIPGFETGVLRGFAAIVERALAKEPDERFPSMRD
                     LAAEIAAVLEEGGWEDEAAPKRPSTGRLSTIPPPVPPLAKRQGFRLAAGIGVALLALV
                     AAGLFLRKGREGEAPAAAPYRPVQLTTSTGLDVFPAFSPDGRTLVYASDRSGRFELYR
                     R"
     assembly_gap    2048..2147
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2148..3791
                     /locus_tag="P5164_10895"
     CDS             <2148..3791
                     /locus_tag="P5164_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HRY44444.1"
                     /translation="RTLVYASDRSGRFELYRRSLAPGGREIAITTDGRQNLSPAWSPD
                     GETIAYHAKSVGGVWLIPALGGVPRQLTTFGSRPSFAPDGSALVFESGSIVDFAANSL
                     GALPPSVLWIAPLDGAPPKPLTRVGRPSGGHGAASWSPDGKRVAFAAYDRTASEIWVV
                     GTNGEDLVQVSEGNGQCYDPVWSADGASITYPCRSAEFAAALYRRPIDPATGRTAGAP
                     EEVATLGSAGTAFVKQLAISRDGARLAWAGLSMASNIWKAPVDGRGERTASEQPVTRE
                     TGRNSRPALSRDGKRIALSRWRTGANQDIWIVDSDGANAVQRTTDPGDDDYPFWFPGD
                     ARLAFMSQRRGHRALFTLDLTTGREELLADFGPGHDAMRLSPDGRRVAFHSNRGGTTL
                     NVWVADLPAGTPRQVTFDRELAAFPCWSPDGRTLAFESKREGDVHVFTVPVSGGTPVQ
                     LTSGSGQSWPYSFSPDGSKVAFAALRDGVWNVGWVTRDGRTLRIVTANERLNSYIRYP
                     EWSPTGEAIYYELAETTGNIWLVDTAARDAAGEKGGGNP"
     gene            3788..4207
                     /locus_tag="P5164_10900"
     CDS             3788..4207
                     /locus_tag="P5164_10900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRY44445.1"
                     /translation="MSETVFTKIIRGEIPCHRVFEDERVFAFLDIAPLAPGHVLVVPK
                     EPAPTLDALSEESAAAIGRVLPRIARAVVAVTGTRDFNVLQNNGAAAHQAVPHVHFHI
                     IPKPAGGGLGIDWPSGRLDGDEGRRLAAEISARLATG"
     gene            complement(4217..5233)
                     /locus_tag="P5164_10905"
     CDS             complement(4217..5233)
                     /locus_tag="P5164_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44446.1"
                     /translation="MPPGADLLPRLFGLWVHLLERTGGASRLAALPVPIAPASRPLAV
                     LLAGVTAVLLLQAALASGRDVTVRAFRAATLLAALALPLAALRLVPASRGPALGGAGL
                     LVVALSSIAHRALASRRAEGTASRLLARVRLLLEGTGLALSGVALGLVVAGRSLPLRL
                     AFWSLFLLRLSIADLIDPSRLAARTGLAGSAARDARSALGRVRRPPRPLRRLRRALSA
                     AAKGSLLLLWLALPLAAALAPGEVAAGAWPRWALQLRWYPPLALGLTALLLLGQAGHA
                     LRGGRPLDAARGAAVGIGTAAWLALAYRDGAFTAWRSALPGLVLAEAVAGFLLGAAAR
                     GRAR"
     gene            5397..6200
                     /locus_tag="P5164_10910"
     CDS             5397..6200
                     /locus_tag="P5164_10910"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HRY44447.1"
                     /translation="MPQTLSLGAVGAAVTELQKALNGLPSRLARLTTDGIFGQKTRSR
                     VVEFQGDNGLDPDGVVGPLTWEKLLALVSALTEIPQTNDLGRIAVVAVARAEASTPGV
                     AAKLAGGTDPSDPRKRSYRMGHTRLLKYFRVTGAAKATPFMEQAVTYLEKSGQLAPMP
                     HWCGIFALWAVKTAGMNVGGWNLGKGISSVTGFRQTHSPAPGDVGYETLGEFQHHFLI
                     EKVVPEGKLLRLRTIEGNSPPNSNHKLAFDRVHDPSSASPQYRFYTCFG"
     gene            6285..>7707
                     /locus_tag="P5164_10915"
     CDS             6285..>7707
                     /locus_tag="P5164_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY44448.1"
                     /translation="MASSSYTAADITVLEGLEPVRKRPGMYIGGVDSRGLHHLVWEIV
                     DNAVDEVINGYASRIEVRLGPDGREVEVYDNGRGIPVDVHPKYKKSALELILTTLHAG
                     GKFEHGSYVHSGGLHGVGSSVVCALSSEMAVNVRRDGHEWRQTYGQGKATSKVTKVST
                     EKVRGSGTTVRFRPDPEIFGKLAFDADEIRDRLEAKTYLHRGLKIVFVDETKKPAVRE
                     EFAHDGGLADYLLKVVAERGKAPTHANGFLLARDADPRLELSLQWTESTDEHLRSWVN
                     GIPTPSGGAHENGLKAGLVKAVRNHLGTHELLPKGVNPSAEDIREGLVALLSVYVLEP
                     QFQGQTKERLNNPEVAAQVDGAVRPALENWLNENPSVAEQIVGRIVMATRAREASRAA
                     AQQVQRKTAISHRLNLPGKLADCSSTDPAESELFIVEGDSAGGSAKQGRDRRTQAILP
                     LRGKVLNAEQANSAKVAGNKELTD"
BASE COUNT         1149 a         2713 c         2818 g          927 t
ORIGIN      
        1 tgtcgaccgg gaccgcgaag ccgatcccgg cgctcgagcc gctcgtcgag gcgatcgccg
       61 tgttcacgcc gatgaggcgc ccggcgctgt cgaggagagg gccgccggag ttccccgggt
      121 tgatggcggc gtccgtctgg acgaccccct cgatctgccg gcccgtgacc gactcgatcg
      181 accggccgag agcgctgacg atccccgtcg agagggactg gtcgagcccg aacgggttgc
      241 cgatcgcgaa gaccttctgg ccgacgagga ggtccttcga cgtgccgacc aggatcggcc
      301 ggagcgtgcg cccggtgggg aagatccgga ggacggcgag gtcccggtcg ggcgccccgc
      361 cgacgaacga agccggccag gtggagccgt cggcgagcgt gacgcgggct gacgaggccc
      421 cctggatgac gtggaagttc gtgacgacgt ggccgtccgt gtcccagacg aagccggagc
      481 cctccccccg cgggatctcc gcgatccgga agagccccgt tcggcggcgg gagagggtcg
      541 tgatgaagac gaccgacggg ctcgactgcc ggaagaggtc gatcgtcgcc cgctcgtcgg
      601 ccgcgaggtc cccgcgcgcc gccacgggcc gcggctcggc ctcggacgcg gcgggacgga
      661 gctttcggca ggccggcgcg gcgaggagcg cggcgaggag aagagggacg aggaaacggc
      721 gcccgggctt cggggtgtcc gagtgataca tcgaggcctc gaggggcgag tataggagcg
      781 gtccccggtg cgaccgccca gagtaagatc gcgaaagacc acggtgtgcg ccccgaggag
      841 gggcagagaa gagcgtcgag ccggccgatg actgcggaac cgtcctcctc ccttcccccc
      901 ggcgccctcg tcgggcgata ccggatcctc gcgtcggtcg ggagcggggc gatgggggag
      961 gtctaccggg ccgaggacac ctcgctcggc cgcatcgtcg cgctgaagac cctgccgctc
     1021 gacctcgtcg gcacgcccga ccgccgggtc cgcttcggga acgaggggcg ggtgatggcg
     1081 tcgctctccc acccgaacct cgtccaggtc ttcgacgtgg gcgagttcga ggggactccg
     1141 tacctggtcc aggagttcgt cgagggggag acgctcgaag cgatcctcct cgggggcgcc
     1201 gtccccgctc cgaaggcggc ccgctggatc gctcaggccg ccgaggggct cgccgccgtc
     1261 cacgacgccg ggttcctcca tcgcgacatc aagccctcga acctcatggt cgaccggcag
     1321 ggacggatac gcgtcctgga cttcggcctc gcgaaggtcc agtcggtacc ggccaacgag
     1381 cggaagagcc agccctcgct gacgaccgac ggtttcgtcg tcgggaccgc gcagtacatg
     1441 tcgccggagc aggcgagcgg gaagaaggcc gacgcgcgaa gcgacgtctt ctccctcggt
     1501 gcggtcttct acgagctcct ctcggggagc tcaccgttcg gcggagagac cgcggtcgac
     1561 gtcatgtacg cgatcctcca cctgccgacc ccgccgatcc cgggcttcga gaccggagtc
     1621 ctccgggggt tcgccgcgat cgtcgagagg gcgctcgcga aggagccgga cgagcgcttc
     1681 ccctcgatgc gcgacctcgc tgcggagatc gccgcggtcc tcgaggaggg gggctgggag
     1741 gacgaggcgg cgccgaagcg cccctcgacg gggcgcctct cgacgattcc gccccccgtg
     1801 ccgccgctcg ccaagcggca ggggttccgt ctggccgccg gaatcggcgt cgccctcctc
     1861 gcgctcgtgg cggccgggct gttccttcgc aaggggcggg aaggagaagc gcccgccgcc
     1921 gcgccgtacc gcccggtgca gctcacgacg tccaccggcc tcgacgtctt tccggcgttc
     1981 tcgcccgacg ggcggacgct cgtctacgcg tcggaccgct ccggacgttt cgagctctac
     2041 cgccggtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgg acgctcgtct
     2161 acgcgtcgga ccgctccgga cgtttcgagc tctaccgccg gtctctcgct ccgggcggcc
     2221 gggagatcgc gatcacgacg gacggtcgcc agaacctctc tcccgcctgg tcgcccgacg
     2281 gggagacgat cgcctaccac gcgaagtccg tcgggggcgt ctggctcatc cccgcgctcg
     2341 gcggcgtgcc gcgtcagctc acgacgttcg gctcgcgtcc ctccttcgcc ccggacggct
     2401 cggcgctcgt cttcgagtcg ggctcgatcg tcgacttcgc cgcgaactcg ctcggcgcgc
     2461 tccccccgtc cgtcctctgg atcgcccccc tcgacggggc gccgccgaag ccgctgacgc
     2521 gcgtcggccg gccgagcggc ggccacggcg ccgcctcctg gtccccggac gggaagaggg
     2581 tcgcgttcgc ggcctacgac cggacggcct cggagatctg ggtcgtcggc acgaacggcg
     2641 aggacctcgt ccaggtgagc gaggggaacg ggcaatgcta cgacccggtc tggagcgcgg
     2701 acggcgcgtc gatcacgtac ccgtgccggt cggccgagtt cgccgccgcc ctctatcgcc
     2761 ggcccatcga cccggcgacg ggccggacgg ccggcgcccc ggaagaggtc gccactctcg
     2821 gcagcgcggg gaccgcgttc gtcaagcagc tcgcgatctc gcgcgacggg gcgaggctcg
     2881 cctgggcggg tctctcgatg gcgagcaaca tctggaaggc ccctgtcgac ggccggggtg
     2941 agaggacggc gagcgagcag ccggtgacgc gggagacggg ccggaactcc cgaccggctc
     3001 tctcgaggga cggcaagcgg atcgccctca gccggtggcg gaccggggcg aatcaggaca
     3061 tctggatcgt cgattccgac ggcgccaacg ccgtccagcg gacgaccgac cccggcgacg
     3121 acgactatcc gttctggttc ccgggcgacg cgcggctcgc gttcatgtcg cagcgccggg
     3181 ggcatcgggc gctcttcacg ctcgacctga cgaccgggcg cgaggagctc ctcgccgact
     3241 tcggtccggg ccacgacgcc atgcgcctct cgcccgacgg gcgaagggtc gcgttccact
     3301 cgaatcgggg cgggacgacg ctgaacgtct gggtcgcgga ccttcccgcg ggcacgcccc
     3361 gccaggtcac gttcgaccgg gagctcgccg cgttcccgtg ctggtcgccc gacgggcgga
     3421 cgctcgcgtt cgaatccaag cgcgaggggg acgttcacgt cttcaccgtg ccggtgagcg
     3481 gcgggacgcc cgtccagctg acgagcggaa gcgggcagag ctggccctat tccttctcgc
     3541 ccgacggctc gaaagtcgcg ttcgccgccc tgcgggacgg cgtctggaac gtcgggtggg
     3601 tgacgcgcga cggccggacg ctccggatcg tgaccgcgaa cgagaggctg aacagctaca
     3661 tccgctaccc ggagtggtcg ccgacggggg aggccatcta ctacgagctc gccgagacga
     3721 ccgggaacat ctggctcgtc gacacggccg cgcgggacgc ggccggagag aagggcggag
     3781 ggaatccgtg agcgagaccg tcttcacgaa gatcatccgg ggggagatcc cctgccaccg
     3841 cgtctttgag gacgagaggg tcttcgcctt cctcgacatc gcgccgctcg cgccggggca
     3901 cgtcctcgtc gtcccgaagg agcccgcgcc gacgctcgac gcgctctcgg aggagtccgc
     3961 tgccgcgatc ggacgcgtcc ttccgcgcat cgcccgggcc gtcgtcgccg tcaccgggac
     4021 gcgcgacttc aacgtcctgc agaacaacgg cgccgccgcc caccaggcgg tgcctcacgt
     4081 ccactttcac atcatcccga agcccgccgg cggcggcctc gggatcgact ggccgagcgg
     4141 acggctcgac ggagacgagg ggcggcggct ggccgccgag atctcggccc gcctcgcgac
     4201 cggctgagcc gcccggctat cgcgcccggc cgcgcgcggc ggccccgagg aggaagcctg
     4261 cgaccgcctc ggcgaggacg agacccggga gcgcgcttcg ccacgccgtg aacgcgccgt
     4321 cgcgataggc gagcgccagc cacgccgccg tgccgatccc gacggcggcg ccccgggcgg
     4381 cgtcgagagg ccggcccccg cggagcgcgt gccccgcctg cccgagcagg aggagggccg
     4441 tgagcccgag cgcgaggggg ggataccagc ggagctggag agcccagcga ggccacgccc
     4501 cggcggcgac ctcgcccggg gcgagcgcgg ccgcgagcgg tagcgcgagc cagaggagga
     4561 gaaggcttcc cttcgccgcc gcggagagcg cacggcggag ccgccggagg gggcgcgggg
     4621 gtctccggac cctcccgagc gcgctccggg cgtcgcgcgc ggccgagccc gccaggcccg
     4681 tccgtgcggc gagacgtgac gggtcgatca ggtcggcgat cgagaggcgg aggaggaaga
     4741 gcgaccagaa ggcgaggcgc agcgggagcg accttcccgc gacgacgagg ccgagagcga
     4801 ccccggagag cgcgagcccc gtcccctcga ggaggagccg gacgcgggcg aggagccgcg
     4861 aagcggtccc ctcggcgcgg cgcgaggcga gggcgcggtg ggcgatcgag gagagcgcga
     4921 cgacgaggag cccggcgccg ccgagagccg gcccgcgcga ggccgggacg agacggaggg
     4981 cggcgagcgg aagggcgagg gcggcgagga gcgtcgctgc gcggaacgcc cggacggtca
     5041 cgtcgcgccc ggaggcgagc gcggcctgga gcagcaggac cgccgtgacg ccggccagga
     5101 gaaccgcgag aggccgcgag gctggcgcga tcggaacggg gagcgccgcg aggcgggagg
     5161 cgccgccggt cctctcgaga aggtggaccc agagcccgaa gaggcgaggc aggaggtcgg
     5221 ctccgggcgg cacccgggga ggttagccgg aaccggagcc ggaggcggcg ccggagccgg
     5281 gcgcgggtcg aggtgacctc cgtcaccggg cggaaggttt cggcggcaat cgactccgtg
     5341 cggccctaga ttgacacgga gtcgcacccg gggaccggga agcacgagga gccgcgatgc
     5401 cccagaccct ctcgctcggc gccgtcggcg ccgccgtgac cgagctgcag aaagcgctga
     5461 acggcctgcc gtcccggctc gcccgcctga cgaccgacgg gatcttcggc cagaagacgc
     5521 gaagccgggt ggtggaattc cagggggaca acgggctcga cccggacggc gtcgtcggcc
     5581 cgctcacctg ggagaagctc ctggccctcg tctccgcgct caccgagatt ccgcagacga
     5641 acgacctcgg ccggatcgcc gtcgtcgccg tggcgcgggc ggaagcgtcg accccgggcg
     5701 tcgcggcgaa gctcgcgggc ggcacggacc cgtccgaccc gcgcaagcgc tcgtaccgga
     5761 tgggacacac gcggctgctc aagtacttcc gggtgacggg cgcggcgaag gcgacgccgt
     5821 tcatggagca ggcggtgacc tacctcgaga agagcggcca gctcgcgccg atgccgcact
     5881 ggtgcggcat cttcgctctc tgggccgtga agaccgcggg gatgaacgtc ggcgggtgga
     5941 acctcgggaa ggggatctcg tccgtgaccg gattccgcca gacccactcg cctgcgcccg
     6001 gggacgtcgg ctacgagacg ctcggggagt tccagcacca cttcctgatc gagaaggtcg
     6061 tgccggaagg gaagctcctc cggctccgca cgatcgaggg gaacagcccg ccgaacagca
     6121 accacaagct cgcgttcgac agggtccacg acccgtcgtc cgcgagcccg cagtaccgct
     6181 tctacacctg cttcggctga gccgggccgg cggcgtccgc cgccgtcgct ccccgggtgt
     6241 cgcttgacgg accccggccg gctgcgtacc gtcggccgcc cgcgatggcc tcttccagct
     6301 acacagccgc cgacatcacc gttctcgaag ggctcgagcc cgtccggaag cgtccgggca
     6361 tgtacatcgg cggggtcgat tcccgcggcc tgcaccacct cgtctgggag atcgtcgaca
     6421 acgccgtcga cgaggtcatc aacggctacg cctcgcggat cgaggtccgc ctcgggccgg
     6481 acggacgcga ggtcgaggtc tacgacaacg ggcgcggcat cccggtcgac gtccacccga
     6541 agtacaagaa gtcggccctc gagctgatcc tgacgacgct ccacgccggc gggaagttcg
     6601 agcacgggag ctacgtccac tcgggcggcc tccacggcgt cggctcgtcc gtcgtctgcg
     6661 ccctctcctc ggagatggcc gtgaacgttc gccgcgacgg gcacgagtgg cggcagacgt
     6721 acgggcaggg gaaggcgacc tcgaaggtga cgaaggtctc gacggagaag gtgcgcggaa
     6781 gcgggacgac cgtccgcttc cgccccgacc cggagatctt cgggaagctc gccttcgacg
     6841 ccgacgagat ccgcgaccgc ctcgaggcga agacctacct ccaccgcggc ctgaagatcg
     6901 tcttcgtcga cgagacgaag aagccggccg tccgcgagga gttcgcgcac gacggcggcc
     6961 tcgccgacta cctcctgaag gtcgtcgccg aacgcggcaa ggcgccgacc cacgcgaacg
     7021 ggttcctcct cgcgcgcgac gccgatccgc gcctcgagct ctccctccag tggaccgagt
     7081 cgaccgacga gcacctccgc tcctgggtca acggcatccc gacgccctcg ggcggggcgc
     7141 acgagaacgg cctgaaggcc ggactcgtga aagccgtgcg gaaccacctc ggcacgcacg
     7201 agctcctccc gaagggagtg aacccgtcgg ccgaggacat ccgcgagggg ctcgtcgcgc
     7261 tcctctcggt ctacgtcctc gagccgcagt tccaggggca gacgaaggag cggctcaaca
     7321 acccggaggt cgcggcgcag gtcgacggcg ccgtccgccc cgcgctcgag aactggctga
     7381 acgagaaccc ctccgtcgcc gagcagatcg tcgggcggat cgtcatggcg acgcgcgccc
     7441 gtgaagcctc gcgcgcggcg gcccagcagg tgcagcggaa gacggcgatc tcccaccggc
     7501 tcaacctccc cgggaagctc gccgactgct cctcgaccga cccggccgag tcggagctct
     7561 tcatcgtcga aggggactcg gccggcggct cggccaagca ggggcgcgac cggcggacgc
     7621 aggcgatcct cccgctccgg ggcaaggtgc tcaacgccga gcaggcgaac tcggcgaagg
     7681 tcgccgggaa caaggagctg accgaca
//
LOCUS       DAOTEZ010000187         7700 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_6723_length_7700_cov_4.866710, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000187 DAOTEZ010000000
VERSION     DAOTEZ010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6723_length_7700_cov_4.866710"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..932
                     /gene="tsf"
                     /locus_tag="P5164_10920"
     CDS             33..932
                     /gene="tsf"
                     /locus_tag="P5164_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009145084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRY44449.1"
                     /translation="MIKELREKTGAGILDCKSALAEANGSIEEAEKSLRKKGLAAAAK
                     KAGRVAAEGLVGYACDGSVAALVELNCETDFVAKTDDFKAARAALAAFVAAEASFGDA
                     PEGNAEALKAMPCPPAVPGATGTIADWLQAATAKTGENTQLRRFARVVAGEGNALHLY
                     AHPGDKTVVVVETAGCDAELGRDLAMQVAALTPSWAVREQVPEKVLADEREIARAQAV
                     ASGKPENVVEKMVEGRIGKFFGEAVLVDQPFVKDDSKKVAQVVAERGGKDAKVVRFVR
                     YKVGEGVEKKQTDLAAEVAALTK"
     gene            945..1688
                     /gene="pyrH"
                     /locus_tag="P5164_10925"
     CDS             945..1688
                     /gene="pyrH"
                     /locus_tag="P5164_10925"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014402200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRY44450.1"
                     /translation="MGSETGRPVYRRVLLKLSGEALLGGQPFGIEPAMVARLADEVTG
                     VARMGVQVAVVVGGGNIIRGVSAATQGIDRVTGDNMGMLATVINALALQDAAERRGVP
                     TRVMTAFEVRSVAEPFIRRRAERHLDKGRVIILAGGTGNPFFTTDSAAALRASELRAD
                     AILKGTKVDGIYSADPKTEPAAVLLPHVTYQEVLERRLGVMDAASIALCRESRIPIRV
                     FNLLVPGNIQRVVTGEPLGSTVDAGEPAN"
     gene            1714..2274
                     /gene="frr"
                     /locus_tag="P5164_10930"
     CDS             1714..2274
                     /gene="frr"
                     /locus_tag="P5164_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRY44451.1"
                     /translation="MPLAEIRKETERRMTTSIDALKAELKHLRTGRASVSLLEGVHVE
                     YYGTPTPLNQVANLSTPDATLILIQPWEAQLCPLIEKAIRNSDLGLNPSSDGRVVRVP
                     VPSPTEERRKEIVKKAHTLGEQSKVEIRHHRHEANDKVKKEGKAGAVSADEEKKGLDD
                     VQKLTDRFVAEVDAVVKAKEADILAR"
     gene            2282..2962
                     /gene="ispD"
                     /locus_tag="P5164_10935"
     CDS             2282..2962
                     /gene="ispD"
                     /locus_tag="P5164_10935"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HRY44452.1"
                     /translation="MDVVALIPAAGLGTRFHEAGPKALVRLAGRPFLLHALERLAASG
                     RVDRACVAVSPGHEASFAAALSGFRAIPVTTTPGGETRRDSVVAAWRASGASDDDLLC
                     VHDAARPLVDPADVAAVVDAAALHGAALAGSPVTDTIKRVQGGVVVETVPRHDLFAAA
                     TPQVMQAGLLRAAVEAGLADATDDVELLERRGVAVRAVVTSRWNVKITFPEDLARAEA
                     WLAAEGRR"
     gene            2959..3432
                     /gene="ispF"
                     /locus_tag="P5164_10940"
     CDS             2959..3432
                     /gene="ispF"
                     /locus_tag="P5164_10940"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009278390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRY44453.1"
                     /translation="MSLRIGQGLDAHPLVAGRRCVLGGVEIPSDAGPEGHSDGDVVLH
                     ALADALLGTAAAGDLGSVFGTTDPRFHDAPSSVFVTEALARAGSPRVVNVDVTLIAER
                     PRVGPYRDAMRAVVASLLGVPVERVSVKASSGNGMTGFGRGEGVFASAVVLVEVP"
     gene            3429..4154
                     /gene="rlmB"
                     /locus_tag="P5164_10945"
     CDS             3429..4154
                     /gene="rlmB"
                     /locus_tag="P5164_10945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRY44454.1"
                     /translation="MIVAGFHPVAAALAERPEAVEAILLQDGRRDARARELERTAREK
                     GIPVRFVPREELDRTAGKAHNGVAARVATRDYDPLEEALAGEPGTRLVLFLDEVTDPG
                     NLGSILRSAAAAGASVVLPERHSVGLSESVSKTSAGALERVKVARVGNAARFLEDVKK
                     DRFWVFGASPEGEPMWDVDLTGDVVLCLGAEGPGLRRLTRETCDRLVAIPMAPGAGSI
                     NVAVSAAVLLFEALRQRRPPSGR"
     gene            complement(4177..7011)
                     /locus_tag="P5164_10950"
     CDS             complement(4177..7011)
                     /locus_tag="P5164_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44455.1"
                     /translation="MRPLLAIPVGVALSLSFRLPAADLVVPVVLDVRSGAAHYRTELI
                     LTNRGGSAVALTLAYRGSIGGAAGSASAEVGAGAQRTIPDVVAWLEAHGVSFPGLREG
                     APQAGTLRITVPDGALATLAVLARTTTPTSAPHPEGAAGLAYPAVGPSSAVAGRAVVH
                     GLRATATERSNLAVWNPGASPVTVRIVAASGTGDGREVVVADGWPLGPGDWRQVDGVL
                     SKAGLLQGWAVVERVEGGVFSAYGVVNDEGTNDGSYLEAAADAATSEAIVVPVLVETP
                     GFRTELILANRGAAAALLELDYRESLSPAAGPGGRATVLLAPREQRIVADAFTWLRSL
                     GIVAGPRGTAPRAGRLRVTGPPGATGLWAAARVVSPSPGGGAYGVFLPGARPAPVPGS
                     EAWIAGLRSDGEARSNVAFVHAGPPGSGAVELEIRAFDGLAGGAPAGEPARYTLAEGE
                     WRQLADPLRALGVASGWVRVTRSAGTAPWLSYGVLNDGSAPGLRTGDGAYVPAAASTS
                     APAGRIAPADLSYLGAFRLPAGGERPLTFEYGGNAMTFRPGGDPGGGGDGFPGSLFVS
                     GHDRMPYGELPDGSRVAEVAIPAPVVSRDLEALPVARLLQPLTDVTGGLFAGIDEIPR
                     LGMQFLDRPETGPRIHLAWGEHLPPPPFRPTHAWFEPDLSAPRPRGAWFLEGLSPWEG
                     NGYLLEIPDAWASAHVSGRVLGTGRYRDGGMAGMGPALFAYRPWLDAAGTPPAPGATL
                     PATLLLRYASTLETEKVERCLVGTQHCDAWEGAAWLTTASGKSAVLFAGTKGVGARFW
                     YGFLNPAGPDLPCVAGDFVNQFTTCRLADGTPCPASEMVECAGHTSSRGWWSSRFASR
                     LLLYDPDDLARVASGESEPWEPQPYAHLDVDPFLYLNPSGVDLADVGPGVQQRFRLGD
                     VTFDRASGLLYLLELFADGARPVVHVFRVR"
     gene            7193..7268
                     /locus_tag="P5164_10955"
     tRNA            7193..7268
                     /locus_tag="P5164_10955"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7226..7228,aa:Thr,seq:tgt)
     gene            7337..7424
                     /locus_tag="P5164_10960"
     tRNA            7337..7424
                     /locus_tag="P5164_10960"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7371..7373,aa:Tyr,seq:gta)
     gene            7487..7562
                     /locus_tag="P5164_10965"
     tRNA            7487..7562
                     /locus_tag="P5164_10965"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7520..7522,aa:Thr,seq:ggt)
     gene            7628..7699
                     /locus_tag="P5164_10970"
     tRNA            7628..7699
                     /locus_tag="P5164_10970"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7660..7662,aa:Gly,seq:tcc)
BASE COUNT         1124 a         2755 c         2863 g          958 t
ORIGIN      
        1 gacgggagag cccgatggaa atcaccaccg ccatgatcaa ggagctccgg gagaagaccg
       61 gagccggcat cctcgactgc aagtcggccc tcgccgaggc gaacgggtcg atcgaggagg
      121 ccgagaagtc gctccgcaag aagggcctcg ccgccgcggc caagaaggcc ggccgcgtcg
      181 ccgccgaggg gctcgtcggc tacgcctgcg acggctccgt cgccgcgctc gtcgagctga
      241 actgcgagac cgacttcgtc gccaagaccg acgacttcaa ggccgcgcgc gcggccctcg
      301 cggcgttcgt cgccgccgag gcctctttcg gcgacgcccc cgaggggaac gccgaggcgc
      361 tcaaggcgat gccctgcccg ccggccgtcc ccggggcgac cgggacgatc gccgactggc
      421 tccaggccgc cacggcgaag acgggcgaga acacccagct ccgccgcttc gcccgcgtcg
      481 tcgccggcga ggggaacgcg ctgcacctct acgcccaccc cggcgacaag accgtcgtcg
      541 tcgtcgagac ggcgggctgc gatgcggagc tcggccgcga cctcgccatg caggtcgccg
      601 cgctgacgcc ctcctgggcc gtccgcgagc aggttcccga gaaggtcctc gccgacgagc
      661 gcgagatcgc gcgggcccag gccgtcgcct cgggcaagcc ggagaacgtc gtcgagaaga
      721 tggtcgaggg ccggatcggg aagttcttcg gcgaggcggt cctcgtcgac cagccgttcg
      781 tcaaggacga ctcgaagaag gtcgcccagg tcgtcgccga gcgcgggggc aaggacgcga
      841 aggtcgtccg cttcgtccgc tacaaggtgg gcgagggcgt cgagaagaag cagaccgacc
      901 tcgccgcgga ggtcgcggcg ctgacgaagt agaacggaac gccgatgggc agcgagacgg
      961 gccggccggt ctaccgccgg gtcctcctca agctctccgg ggaggccctc ctcggagggc
     1021 agcccttcgg catcgagccg gcgatggtcg cgcgcctcgc cgacgaggtg accggcgtcg
     1081 cccggatggg cgtgcaggtc gccgtcgtcg tcggcggggg gaacatcatc cgcggcgtct
     1141 cggccgcgac gcaggggatc gaccgggtga cgggcgacaa catggggatg ctcgccacgg
     1201 tcatcaacgc gctcgcgctc caggacgccg cggagcggcg gggcgtgccg acccgggtca
     1261 tgacggcctt cgaggtccgc tccgtcgccg agccgttcat ccggcggcgg gcggagcggc
     1321 acctcgacaa gggacgggtc atcatcctgg ccggcggcac ggggaacccc ttcttcacga
     1381 ccgactcggc cgccgcgctc cgcgcgagcg agctgcgggc cgacgcgatc ctgaagggga
     1441 cgaaggtcga cggcatctac agcgccgacc cgaagacgga gcccgccgcc gtccttctcc
     1501 cgcacgtcac ctaccaggag gtcctggagc ggcggctcgg cgtcatggac gccgcctcga
     1561 tcgccctctg ccgggagagc cgcatcccga tccgggtctt caacctcctc gtccccggga
     1621 acatccagcg cgtcgtgacg ggcgagccgc tcggctcgac cgtcgacgcc ggcgaacccg
     1681 ccaactgaat cgagaccaga gaggagatag ccgatgcccc tcgccgagat ccgcaaggaa
     1741 accgagcgcc ggatgacgac ctcgatcgac gccctgaagg ccgagctgaa gcacctccgg
     1801 acgggccgcg cctccgtctc cctgctcgag ggggtccacg tcgagtacta cgggacgccg
     1861 acaccgctga accaggtggc gaacctctcc actcccgacg cgacgctgat cctcatccag
     1921 ccctgggagg cgcagctctg cccgctcatc gagaaggcga tccgcaacag cgacctcggc
     1981 ctgaacccga gctccgacgg gcgggtcgtc cgcgtgccgg tcccctcccc gaccgaggag
     2041 cggcgcaagg agatcgtcaa gaaggcccac accctcggcg agcagtcgaa ggtcgagatc
     2101 cgccaccacc gccacgaagc caacgacaag gtcaagaagg aagggaaggc gggcgcggtc
     2161 tcggccgacg aagagaagaa ggggctcgac gacgtccaga agctgaccga ccggttcgtg
     2221 gccgaggtcg acgcggtcgt caaggcgaag gaagcggaca tcctcgcacg gtgaccgtga
     2281 cgtggacgtc gtcgcgctga tcccggcggc gggtctcggg acgcgtttcc acgaggcggg
     2341 ccccaaggct ctcgtgaggc tcgcggggag gcccttcctc ctccacgctc tcgagcggct
     2401 cgccgcctcg gggcgcgtcg atcgcgcctg cgtagcggtc tcgccaggcc acgaggcgtc
     2461 cttcgccgcg gccctctccg gcttccgggc gatccccgtg acgacgacgc cgggaggcga
     2521 gacgcggcgc gactccgtcg tcgccgcctg gcgcgcttcg ggcgcgtcgg acgacgacct
     2581 cctctgcgtc cacgacgcgg cgcgccccct cgtggatccg gccgacgtcg cggccgtcgt
     2641 cgacgcggcc gccctgcacg gagccgccct cgcgggaagc cccgtgacgg acacgatcaa
     2701 gcgggtccag ggcggcgtcg tcgtcgagac ggtcccccgg cacgatctct tcgcggcggc
     2761 gacaccccag gtgatgcagg cgggtctcct ccgcgcggcg gtcgaggccg gcctcgccga
     2821 cgcgacggac gacgtcgagc tcctcgagcg gcgcggcgtc gcggtccgcg cggtcgtcac
     2881 gtcgcgctgg aacgtgaaga tcacctttcc cgaggacctc gcgagggccg aggcgtggct
     2941 cgccgcggag gggcggcgat gagcctgagg atcgggcagg ggctcgacgc ccacccgctc
     3001 gtcgccgggc ggcgctgcgt cctcggcggg gtcgagatcc cgtccgacgc cggccccgag
     3061 gggcactcgg atggcgacgt cgtcctccat gccctcgcgg acgcgctcct cggaacggcc
     3121 gcggcgggcg acctcgggag cgtcttcggg acgaccgacc cgcgcttcca cgacgccccg
     3181 tcgtccgtct tcgtgaccga ggcgctcgcg agggcgggga gcccgagggt cgtgaacgtc
     3241 gacgtcacgc tgattgccga acggccgcgc gtcgggccgt accgggacgc gatgcgggcc
     3301 gtcgtcgcct cgctcctcgg cgtccccgtc gagcgcgtct ccgtgaaagc ctcgagcgga
     3361 aacggcatga ccggcttcgg ccgcggcgag ggggtcttcg cctcggcggt cgtcctcgtc
     3421 gaggtcccgt gatcgtcgcc gggttccacc cggtcgccgc ggcgctcgcc gagcggccgg
     3481 aggccgtcga ggcgatcctc ctccaggacg ggcgtcgcga cgcgcgggcg cgcgagctgg
     3541 agaggacggc gcgcgagaag gggatcccgg tccgtttcgt cccgcgcgag gagctcgacc
     3601 ggaccgcggg gaaggctcac aacggcgtcg ccgcgcgcgt ggccacccgc gattacgacc
     3661 ctctcgagga ggcgctcgcc ggcgagcccg ggacgcgcct cgtcctcttc ctcgacgagg
     3721 tgaccgatcc ggggaacctc ggatcgatcc ttcgctccgc ggcggcggcc ggcgcctccg
     3781 tcgtcctccc cgagcggcac tccgtcggcc tttccgagtc ggtctcgaag acgagcgccg
     3841 gggcgctcga gcgggtgaag gtcgcccgcg tcggaaacgc ggctcgcttc ctcgaggacg
     3901 tcaagaagga ccgcttctgg gtcttcggcg cctcgccgga gggcgagccg atgtgggacg
     3961 tcgacctgac cggcgacgtc gtcctctgcc tcggcgccga ggggccgggc ctccggcgcc
     4021 tgacgcgcga gacgtgcgac cgtctcgtgg cgatcccgat ggcgccgggc gccggctcga
     4081 tcaacgtcgc ggtctccgcc gcggtcctcc tcttcgaggc gctccggcag cgtcggccgc
     4141 cgtccggccg ctgagcgacc ccgcggggcg gcgtcctcag cggacgcgga agacgtggac
     4201 gacggggcgc gccccgtccg cgaagagctc gaggaggtag aggaggccgc tcgcgcggtc
     4261 gaacgtcacg tcgccgaggc gaaagcgctg ctggaccccc ggcccgacgt cggcgaggtc
     4321 gacgccggac gggttcaggt agaggaatgg gtcgacgtcg aggtgggcgt acggctgcgg
     4381 ctcccacggc tccgactcgc cggaagcgac ccgcgcgagg tcgtccgggt cgtagagcag
     4441 gagccgtgag gcgaagcggc tgctccacca tccgcggctc gacgtgtgcc ccgcgcactc
     4501 gaccatctcc gatgcggggc acggcgtccc gtccgcgagg cggcaggtcg tgaactggtt
     4561 cacgaagtcg cccgccacgc aggggaggtc cgggcccgcg gggttgagga agccgtacca
     4621 gaagcgggcg cccaccccct tcgtccccgc gaagaggacg gccgacttcc cggaggccgt
     4681 cgtgagccag gccgccccct cccacgcgtc gcagtgctgc gtcccgacca ggcatctctc
     4741 gaccttctcc gtctcgaggg tcgaggcgta gcggaggagg agcgtggccg ggagcgtcgc
     4801 gccgggggcg ggcggcgttc cggcggcgtc gagccacggc ctgtaggcga agagcgccgg
     4861 ccccatgccg gccatcccgc catcccggta gcggcccgtg ccgaggacac ggccggagac
     4921 gtgcgcgctc gcccaggcgt ccgggatctc gagcaggtag ccgtttcctt cccacggcga
     4981 gagcccctcg aggaaccagg ctcccctcgg gcgcggagcg gagaggtcgg gctcgaacca
     5041 ggcgtgcgtg gggcggaagg gcggcggcgg aaggtgctct ccccaggcga ggtgaatccg
     5101 cggtccggtc tcgggccggt cgaggaactg catgccgagc cgagggatct cgtcgatccc
     5161 ggcgaagagg ccgccggtga cgtccgtcag aggctggagc agccgggcaa cggggagcgc
     5221 ctcgaggtcg cgcgagacga cgggagccgg aatggcgacc tccgcgaccc ggcttccgtc
     5281 ggggagctcg ccgtagggca tccggtcgtg gcccgagacg aagagggagc cggggaagcc
     5341 gtcgccgcca cctcccgggt cgccccccgg ccggaaggtc atcgcgtttc ccccgtattc
     5401 gaacgtgagg ggacgctctc ctccggccgg gaggcggaag gcgccgaggt aggagagatc
     5461 ggcgggcgcg atccgtccgg ccggcgcgga cgtgctcgcg gcggccggga cgtacgcgcc
     5521 gtctcccgtc cggaggccgg gcgcggagcc gtcgttgagg acgccgtacg agagccacgg
     5581 agccgtccct gcactccgcg tgacgcggac ccatccggaa gcgacgccga gagcgcggag
     5641 cgggtcggcg agctggcgcc actctccctc cgcgagcgtg taccgcgcgg gctcgccggc
     5701 gggcgccccg ccggcgagcc cgtcgaaagc gcggatctcg agctcgacgg cgcccgaccc
     5761 gggtggtccg gcgtggacga aggcgacgtt ggaccgagct tctccgtccg accggaggcc
     5821 ggcgatccag gcttcggatc cggggaccgg tgcgggccgg gcgccgggca ggaagactcc
     5881 atacgccccg cctccgggag agggggaaac gacccgtgcg gcggcccaga gtccggtcgc
     5941 gccgggcggc cccgtcaccc ggagccggcc tgcgcgaggc gcagtgcccc gcggcccggc
     6001 cacgattccg aggctgcgca gccaggtgaa ggcatcggcg acgatccgct gctcgcgggg
     6061 cgcgagcagc accgttgccc ggcctcccgg gccggcggca ggcgagaggg actcccggta
     6121 gtcgagctcg aggagcgcag ccgccgcgcc gcggttcgcg aggatcagct cggttcggaa
     6181 tccgggcgtt tcgacgagga cggggacgac gatcgcctcg gaggtggccg cgtcggccgc
     6241 cgcctccagg tacgagccgt cgttcgtccc ctcgtcgttc acgacgccgt acgcggagaa
     6301 gacgcctccc tcgacccgct ccacgaccgc ccagccctga aggaggccgg ccttcgacag
     6361 gacgccgtcg acctgccgcc agtcgcccgg cccgaggggc cagccgtcgg ctacgacgac
     6421 ttcccggccg tctcccgttc cgctcgcggc gacgatccgg accgtgacgg gggaggctcc
     6481 gggattccag acggccaggt tcgaccgctc cgttgccgtc gcccgcagcc cgtgaacgac
     6541 ggctcggccc gcgaccgcgg aagacggacc cactgcgggg taggcaaggc ccgcggcgcc
     6601 ctccgggtgg ggcgcggagg tcggcgtcgt cgtccgggcg agcacggcga gggtcgcgag
     6661 cgctccgtcg ggaacggtaa tccggagcgt accggcttgg ggggcgcctt cccggaggcc
     6721 cgggaacgac actccgtgcg cctccagcca ggcgacgacg tcgggaatcg tccgctgtgc
     6781 ccccgccccg acctccgcgg aggcgctccc ggcggcgccg ccgatcgagc ccctgtacgc
     6841 gagcgtgagg gcgacggcgg agccgccgcg gttcgtcagg atcagctccg tgcgatagtg
     6901 cgcggccccg ctccggacgt cgaggaccac cggaacgacg aggtcggctg ccgggagtcg
     6961 aaacgacaac gaaagggcga ccccgaccgg gatggccaga agcggacgca tcgaaacctc
     7021 cgacagggtc ggctcgaata tggcgtcgcg cggaccttac cgccaggaga cccttcgcgg
     7081 gcgcccccgg cacgggactc gcttgacgga aagcccgagc attgatataa gccacggttc
     7141 gcgccttccg gcttcggccg ggtgtccggc cggaggcctc ggggtgcgac gggctggcgt
     7201 agctcaactg gcagagcagc cgatttgtaa tcggcaggtt gcgggttcaa gtcccatcgc
     7261 cagctccacg ggcgctcccg gaaggggttc cggaccgctc cgtgggaagg agagaggaag
     7321 agacggacgt aaaggcgggt aggtggccga gtggttaatg gcagcagact gtaaatctgc
     7381 cgcgcttagg cgctacgggg gttcaaatcc ctccctgccc accagcactt ccgacatcgg
     7441 ctccggctcc cctcgggggg cgcggggcga ccggtccggc agacgggccc acgtagctca
     7501 ggcggtagag cacgtccttg gtaaggacgg ggtcagcagt tcaattctgc tcgtgggctc
     7561 cagactcctc tcccgggcga tcggccccac cggagagagg gcggaacgaa ggggacgaac
     7621 gccacgggcg ggagtaactc aggggtagag tcacagcctt ccaagctgtt ggtcgcgggt
     7681 tcgaatcccg tctcccgctc
//
LOCUS       DAOTEZ010000188         7657 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_6783_length_7657_cov_5.740726, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000188 DAOTEZ010000000
VERSION     DAOTEZ010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7657
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6783_length_7657_cov_5.740726"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2186)
                     /locus_tag="P5164_10975"
     CDS             complement(<1..2186)
                     /locus_tag="P5164_10975"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRY44456.1"
                     /translation="MPGSRSGRGARFAGALTLLAALALPASAQLPFEVIGLGDGLPQS
                     QLPAMAQDRDGFLWIGTLGGLARFDGNAIESFSTKDGLPSNYVEELLLDDEGTLWIGT
                     VAGVARWRGRRLEPFPAAGTVRCRAIAKDAEGRIWLGTEEGVLRCEKGSCTSVLGGPD
                     RPQATFDVLPTTRGTYAATNRGLFRIEGAVANLEEGPPALGDGGRALAETPEGLWLGT
                     ATRGLWLRTGAEWTPVPLMPRGGRAVFRITLGPSGTFYVATNDSGLFLRRPGSVAFER
                     WSTENGLPSNVVNFAFEDREENVWVGTDVGGLARFRGQLVRNLGAKEGLLSSCVFTMS
                     ETPDGDLWLGTMRGAARLARGAEPRVVEVVGEAQGLANELVFRAERGPDGSLWVWSEG
                     GLQRRRPGEERLGPVDEYGSAISADVWGFAFDGAARLWVGSRRAEGGLAVLRADGTWR
                     VFDRLPDGSRIDVAHALARRRAGGVWAAFGNRLAACDGETIAPLPDPPAAMSSPYVNK
                     LFEDSKGRLWAASGSGLARLETDGTWTLLTERLGSPYVYFVGEDRTGTVWVGTTRGVY
                     RLGAGDAVTPFTPDDGLAGWETNINAFHTDRDGVVWIGTVDGLSRYDPASHRPNAHPP
                     RVVVESVRLPGRTVAFPDRLELAWSERSAAFRVAVLSFRARGRTAYRARLEGLEESWL
                     PLRREPELRYTNLPAGDLTLHVQGINESGLWGEVVTIPIRVRPPFW"
     gene            complement(2323..2568)
                     /locus_tag="P5164_10980"
     CDS             complement(2323..2568)
                     /locus_tag="P5164_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44457.1"
                     /translation="MRQIASLALALALVAAPAALAGPECAGHAKAASCCTKKDGADKK
                     CDPANCKKDAAKCTHADKAKCAEACAKKAEAPKPAKS"
     gene            complement(2697..3113)
                     /locus_tag="P5164_10985"
     CDS             complement(2697..3113)
                     /locus_tag="P5164_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44458.1"
                     /translation="MAYRPAASARSLVALALAAALLPLPLLAAVRRAAPSPASPAPSG
                     CCPSSAPPACPSCPSEGRSSAPTAPKRTGTCCSPAALLPDAASLREREARSRRAEDAD
                     GIRTSRASARAAAEDRRSSVAAASPPPRLLTCTFRN"
     gene            3275..4078
                     /locus_tag="P5164_10990"
     CDS             3275..4078
                     /locus_tag="P5164_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44459.1"
                     /translation="MTLEKISGAVGAFAVTAMLLVPGAALTQEAPPPPPAEVAQAAPA
                     PVPTPAPKPFEEIVKLWKGNLSEEFIKRKIESEAVIYQLSADDIVLCKASNLPESIIE
                     AMMKTAQRPGAAPAVAAPVAPVAAPVAAPAPAAPVPPADLAAQADRSWEGLVRRNDGI
                     VLFKSRWDDGKLSFKEEKLSWLDAKDEKKNLILPARSIKEQFLVCLKGAPSEKTECFE
                     WGVKTENGEYRFRDVGWKRSDSVKPQELFDFMKAIYPNLVSSRYPADAK"
     gene            4210..4734
                     /locus_tag="P5164_10995"
     CDS             4210..4734
                     /locus_tag="P5164_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY44460.1"
                     /translation="MPTRRTKTPANAGPRWGAELTRIELLRLREVLRLAGLTLTPTRA
                     AVLWGLQRSATPATLAELADGLGSKGWARSAVSRAVNDLVAARLVTRTAPPGSGVALL
                     SAAPEPELPEGTEYFLCDGCSVHECLEDAHVVATSGGSGPKALPRAEVTSMVESLCRR
                     REERLRALVPVRAV"
     gene            complement(4765..5463)
                     /gene="lipB"
                     /locus_tag="P5164_11000"
     CDS             complement(4765..5463)
                     /gene="lipB"
                     /locus_tag="P5164_11000"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:TIGR00214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HRY44461.1"
                     /translation="MPRPLEVVSLLPLGPRPYDEVVVLQKEAEARAKAGGPETLFLLE
                     HEDVITAGRNAGTDDLHLPAEQLARLGVSFHRSDRGGKLTFHGPGQLVAYPILRLEGT
                     ERDVRRFVRSLEEVLILTAADFGVGASRSDVPARWSSVWVGNDKLAAIGVHLSDWVTT
                     HGIGLNVTTRLERFDLFVPCGIRDGGVTSLEKACPGVPPPALGDVAARFVARFAEVFG
                     REVEAPAAAARAGA"
     gene            complement(5463..7019)
                     /locus_tag="P5164_11005"
     CDS             complement(5463..7019)
                     /locus_tag="P5164_11005"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY44462.1"
                     /translation="MASAGSEYDEVPYLAPSFANTYPDALAVAALMRGLDAPRLAGAR
                     ILELGCARGANLVPMAWSMPDAELVGIDLSRRQIEAARERASASGTSNVTFHAMDLRD
                     VDASFGAFDFVIAHGLFSWVPRDVQEALLEVCARRLSPNGIVYLSYNTLPGWSTGVMF
                     REMVLYRVRGIADPAERLREARRFLRFLAGAAQPKETIWAAMLDEHASYFEQGHDWYL
                     LHDDLEAENDPVYFHEMVARAEGHGLQYVTEERSLSSGEVLAPEALEQLDRFAANRIE
                     REQVVDFACNRRFRRSLFCRAGRALPPGPTPGRLAACRFRACVKPVDASADPLSPGAE
                     RFLGLDGRSLESSDPRLRALLHVLHGVWPGTLDHAAATHVLAEATRRTGGEAPAPELL
                     DRMVYQAVLGQVVSAHLLDPPLATRLPERPFVSPVARYEAAAEGPVTHLLNGSSELEP
                     LDRFVLRRLDGTRTRDDVAAALREALEAGRLEPTATGGAAEPGEEPMAAEELADLALA
                     RLLAGCYILR"
     gene            complement(7111..>7657)
                     /locus_tag="P5164_11010"
     CDS             complement(7111..>7657)
                     /locus_tag="P5164_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRY44463.1"
                     /translation="PDSIPLNDIILYVVSRVALSHTMCNAHYFDDKMVFFNTVNLGMA
                     VALDWGLIVPVIKNADDLSIVGLARAANDLAGRARTKKLLPDEVQGGTFTITNPGVYG
                     SLFGTPIINQPQVAILNIGTIEKRAKVIELPDGSDTIAVRTMGYLALSFDHRLVDGAE
                     ADAFMGVVKATLENGSFGDLD"
BASE COUNT         1068 a         2727 c         2849 g         1013 t
ORIGIN      
        1 atccagaagg ggggacggac gcgaatcggg atcgtgacga cctcgcccca gagccccgac
       61 tcgttgatcc cctggacgtg gagcgtcagg tctccggcgg ggaggttcgt gtagcggagc
      121 tccggctcgc ggcgaagcgg aagccagctt tcctcgagcc cttcgaggcg ggcgcgatag
      181 gccgtccggc cccgggcgcg gaacgaaagg accgcgacgc ggaaggcggc cgaccgctcc
      241 gaccaggcga gctcgaggcg gtcggggaac gcgaccgtcc gccccgggag ccggaccgac
      301 tcgacgacga cgcgcggcgg gtgggcgttc ggacggtgcg aggccgggtc gtagcgcgag
      361 aggccgtcga ccgtgccgat ccagacgacc ccgtcgcggt cggtgtggaa cgcgttgatg
      421 ttcgtctccc acccggcgag gccgtcgtcg ggcgtgaacg gcgtcacggc gtcgcctgcg
      481 ccgagccgat agacgccccg cgtcgtcccg acccagaccg tccccgtccg gtcctcgccg
      541 acgaagtaga cgtacgggct cccgagccgt tccgtcagga gcgtccacgt cccgtcggtc
      601 tcgagccgcg cgaggccgct gccgctcgcc gcccagaggc gccctttcga gtcctcgaag
      661 agcttgttca cgtaggggct gctcatcgcc gcgggcgggt cggggagcgg ggcgatcgtc
      721 tcgccgtcgc aggcggcgag acgattcccg aacgccgccc agacgccgcc cgcgcggcgc
      781 cgcgccaggg cgtgcgccac gtcgatccgc gagccgtccg ggaggcggtc gaagacgcgc
      841 cacgtcccgt ccgcgcgaag cacggcgagc ccgccctcgg cgcggcggct gccgacccag
      901 aggcgcgcgg cgccgtcgaa ggcgaacccc cagacgtcgg cgctgatcgc cgatccgtac
      961 tcgtccaccg gtccgagccg ctcctcgccc gggcgccggc gctgcaggcc gccctccgac
     1021 cagacccaga ggctcccgtc ggggccgcgc tcggcgcgga agacgagctc gttcgcgagc
     1081 ccctgcgcct cgcccacgac ctcgacgacg cgcggctcgg cgccgcgcgc gagccgcgcg
     1141 gccccccgca tcgtgccgag ccagaggtct ccgtccggcg tctcgctcat cgtgaagacg
     1201 caggaggaca gcagcccctc cttcgccccg aggttccgga cgagctgccc gcggaagcgg
     1261 gcgaggcctc cgacgtcggt cccgacccag acgttctcct cgcggtcctc gaacgcgaag
     1321 ttgacgacgt tcgaagggag cccgttctcg gtgctccagc gctcgaacgc caccgacccg
     1381 ggacggcgga ggaagagccc ggagtcgttc gtcgcgacgt agaacgtacc cgaaggaccg
     1441 agcgtgatcc ggaagacggc ccggccgccg cgcggcatca gcgggaccgg cgtccactcc
     1501 gcgccggttc ggagccagag gccgcgcgtc gccgtgccga gccagagtcc ctccggcgtc
     1561 tctgcgagag cacggccgcc gtcgccgagc gcaggcggcc cctcctcgag gttggcgacg
     1621 gcaccctcga tccggaagag tccgcggttc gtcgccgcgt acgtcccgcg cgtcgtcggg
     1681 aggacgtcga acgtcgcctg ggggcggtcg ggtccgccga ggacggaggt gcaagagccc
     1741 ttctcgcagc ggaggacgcc ctcctccgtc ccgagccaga tccgcccctc cgcgtccttc
     1801 gcgatcgcac ggcaccgcac ggttccggcc gccgggaacg gctccagcct gcgcccgcgc
     1861 caccgcgcga cgcccgcgac ggtgccgatc cagagcgtcc cctcgtcgtc gaggagcagc
     1921 tcctcgacgt agttcgaggg gaggccgtcc ttcgtcgaga aggactcgat cgcgttcccg
     1981 tcgaagcgtg cgaggccgcc gagggtcccg atccagagga acccgtcgcg gtcctgcgcc
     2041 atcgccggga gctgcgactg gggaagcccg tcgccgaggc cgatcacctc gaacgggagc
     2101 tgggcggagg cggggagggc gagcgccgcg agcagcgtga gcgcgcccgc gaatcgggcc
     2161 ccgcgtccgg accggctccc aggcaccgct ccttctcagc cctccggccc gccatcttct
     2221 cacggggccg gccgcgaacc ggcccgcgga acggcccgaa gaaggggcgg gcccggacgc
     2281 cgacggcgtc cgggcccgcc ccggggactc gtatgccggc gctcagctct tcgcgggctt
     2341 cggcgcctcg gccttcttcg cgcacgcctc ggcgcacttc gccttgtcgg cgtgggtgca
     2401 cttcgcggcg tccttcttgc aattcgcggg gtcgcacttc ttgtcggcgc cgtccttctt
     2461 cgtgcagcag gaggcggcct tggcgtggcc ggcgcattcg gggccggcga gagcggccgg
     2521 ggcggcgacg agggccaggg cgagggcgag ggaagcgatc tgacgcatgg agctactcct
     2581 tctcgttgtt tcgttgctcg tgggcgcggc ctcgggccgc gcgcgttccg tcggtcggcg
     2641 ggcggaggcg gtccgctctc gcggcgcgga aggcgcgagg cctcccgcgg gaccggtcag
     2701 ttccggaacg tgcaggtcaa cagccgaggg ggcggggagg cggccgcgac ggacgacctg
     2761 cggtcctccg cggccgcgcg cgcggaggca cgggaggtcc ggatgccgtc cgcgtcctcg
     2821 gcgcggcgcg agcgcgcctc gcgctcgcgg aggcttgcgg cgtcggggag cagggcggcg
     2881 ggagagcagc acgtcccggt cctcttcgga gccgtgggcg cggacgaccg cccctccgag
     2941 gggcaggagg ggcaggccgg cggggcgctc gacggacagc agcccgaagg ggccggggat
     3001 gccggggagg gcgccgctct gcggaccgcg gccaggagcg ggagggggag gagtgccgcg
     3061 gcgagcgcca gcgcgacgag cgatcgtgcc gaagctgcgg ggcggtacgc catcgccccc
     3121 atcctatcga agaagcgtgc caggaatcgg cgttcgcgag gaaaccgagc gcgctgtcgt
     3181 ttcctgagcg agaatgagcc cacagcgagg atcgggtgcg aatcgcccgg ccgggcggtc
     3241 ccggcgcccg atcgagcaga ggaggtacgc actcatgacc ctcgagaaga tctccggcgc
     3301 ggtcggcgcc ttcgcggtta cggcaatgct cctggtcccg ggcgccgcgc tgacgcagga
     3361 agcgccgccg ccgccgcccg ccgaggtcgc ccaggccgcc cccgccccgg tcccgacccc
     3421 ggctccgaag ccgttcgaag agatcgtcaa gctctggaag gggaatctct cggaggagtt
     3481 catcaagcgg aagatcgagt cggaggcggt catctaccag ctttccgccg acgacatcgt
     3541 cctgtgcaag gcctcgaacc tccccgagtc gatcatcgag gcgatgatga agacggcgca
     3601 gcggcccggc gcggcgccgg cggtcgccgc gcccgtcgcg cccgtggccg ccccggtcgc
     3661 ggcgccggct ccggccgcgc cggtgccgcc ggcggacctc gccgcgcagg ccgaccgctc
     3721 ctgggagggc ctcgtccgga ggaacgacgg gatcgtcctc ttcaagagcc gctgggacga
     3781 cggcaagctc tccttcaagg aggagaagct ctcctggctc gacgcgaagg acgagaagaa
     3841 gaacctcatc ctcccggcgc ggtcgatcaa ggagcagttc ctcgtctgcc tgaagggggc
     3901 gccgagcgag aagaccgagt gcttcgagtg gggcgtgaag acggagaacg gcgagtaccg
     3961 gttccgcgac gtcggctgga agcgctccga cagcgtgaag ccccaggagc tcttcgactt
     4021 catgaaggcg atctacccga acctcgtctc gtcgaggtac cccgccgacg cgaagtaggt
     4081 ccggccggct cccgcagccc gtttcgacgc cctctccccg cccgggaggg ggcgttttcg
     4141 tttccggcga gtcgccgaag gcgctcggcg cgcttgcccg cggcaaacct cccatgttac
     4201 acctcgcgcg tgccgacgcg ccggacgaaa actcctgcga acgccgggcc ccgctgggga
     4261 gccgagctga cgcgcatcga gctgctccgg cttcgtgagg tgctccgcct cgccgggctc
     4321 accctgacgc cgacgcgtgc cgccgtcctc tgggggcttc agcgttcggc tacgcccgcg
     4381 acgctcgccg agctcgccga cggcctcggc tcgaaaggct gggcgaggag cgccgtctcc
     4441 cgcgccgtga acgacctcgt cgccgcgcgg ctcgtgacgc ggacggcccc gccggggagc
     4501 ggcgtcgcgc ttctctcggc cgcgcccgag cccgagctgc ccgaggggac cgagtacttc
     4561 ctctgcgacg gctgctccgt gcacgagtgc ctcgaggacg cgcacgtcgt cgcgacctcc
     4621 ggggggagcg ggccgaaggc cctcccgcgc gcggaggtga cctccatggt cgagagcctg
     4681 tgccgtcgcc gcgaggagcg ccttcgcgcc ctcgtcccgg tccgcgcggt ctgaccgggc
     4741 gttctcagcc ggaagcggga agcctcacgc cccggcgcgg gccgccgcgg cgggagcttc
     4801 gacctcccgt ccgaagacct ccgcgaaacg tgcgacgaag cgcgccgcga cgtcgccgag
     4861 ggccgggggc gggacgcccg ggcaggcctt ctccagcgac gtgacgcccc cgtcgcggat
     4921 cccgcacgga acgaagaggt cgaagcgctc gagccgggtc gtgacgttga ggccgatccc
     4981 gtgcgtcgtg acccagtcgg aaaggtggac gccgatggcg gcgagcttgt cgttcccgac
     5041 ccagaccgac gaccagcggg cgggcacgtc gctccgggag gcgcccacgc cgaagtcggc
     5101 cgcggtgagg atcaggacct cctccaggct cctcacgaac cgccggacgt ccctctcggt
     5161 cccctcgagg cgcaggatcg gataggcgac gagctggccc gggccgtgga acgtcagctt
     5221 tccgcctcgg tcggaacggt ggaacgagac gcccagccgg gcgagctgct cggcgggaag
     5281 gtgcaggtcg tcggtcccgg cgtttcgtcc ggcggtgatc acgtcttcgt gctcgagaag
     5341 gaacagggtc tccggtccgc ccgccttcgc gcgcgcttcg gcctccttct ggagaacgac
     5401 gacctcgtcg tacgggcggg gaccgagcgg gaggagggag acgacctcga gggggcgcgg
     5461 catcaacgga gaatgtagca gccggccagg agccgggcga gggcgaggtc ggcgagctcc
     5521 tcggcggcca tcggctcctc gccgggttcc gccgcgccgc ccgtcgccgt cggctccagc
     5581 cttccggctt cgagcgcctc ccgaagcgcg gcggcgacgt cgtcccgggt ccgcgtcccg
     5641 tcgagccgtc ggagcacgaa gcggtcgagc ggctcgagct cgctcgagcc gttgagcagg
     5701 tgcgtcaccg gaccctcggc cgccgcctcg taccgggcga ccggagagac gaagggtcgc
     5761 tcggggagcc gggtcgcgag cggcgggtcg aggaggtggg cggagacgac ctggccgagc
     5821 acggcctgat agaccatccg gtcgaggagc tcgggcgcgg gcgcttcgcc gcccgtccgg
     5881 cgcgtcgcct ccgcgaggac gtgggtggcc gccgcgtggt cgagggtccc gggccagacg
     5941 ccgtgcagga cgtggaggag cgcgcgcagg cgtgggtccg acgactcgag agagcgtccg
     6001 tcgagcccca ggaatcgctc ggctccgggc gagagcgggt ccgccgaagc gtcgacgggc
     6061 ttcacgcatg cccggaatcg gcacgccgcg agccggccgg gcgtcggtcc gggcgggaga
     6121 gcgcgtccgg cacggcagaa gaggctcctg cggaagcgtc ggttgcaggc gaagtcgacg
     6181 acctgctccc tctcgatccg gttcgcggcg aatcgatcca gctgctcgag cgcctcgggc
     6241 gcgaggacct cgcccgagga aagggacctc tcctcggtga cgtactggag cccgtgcccc
     6301 tcggccctcg cgaccatctc gtggaagtag acggggtcgt tctcggcctc gaggtcgtcg
     6361 tggaggaggt accagtcgtg cccctgctcg aagtacgacg cgtgctcgtc cagcatcgcc
     6421 gcccagatcg tctctttcgg ctgcgccgcg ccggcgagga aacggaggaa cctcctcgcc
     6481 tcgcggagcc gctccgcggg gtcggcaatt ccccggaccc tgtagagcac catctctcgg
     6541 aacatcaccc ccgtgctcca gcccggcagg gtgttgtagc tgaggtagac gatcccgttc
     6601 ggggagagcc gccgggcgca gacctcgagg agcgcctcct ggacgtcccg cgggacccac
     6661 gagaagaggc cgtgagcgat gacgaagtcg aacgcgccga aggacgcgtc gacgtctcgc
     6721 aggtccatcg cgtggaacgt gacgttggac gtgccggacg ccgaggcccg ctcccgggcc
     6781 gcctcgatct gccggcgtga gaggtcgatt ccgacgagct cggcgtccgg catcgaccag
     6841 gccatcggca cgaggttcgc gccgcgtgcg cacccgagct cgaggatgcg cgcccccgcg
     6901 aggcggggcg cgtcgaggcc gcgcatcagc gcggccaccg cgagagcgtc ggggtacgtg
     6961 ttcgcgaagg acggggcgag gtacgggacc tcgtcgtact ccgatccggc agaggccatc
     7021 gcccaacctc ctcgcgcctc gacccggctc tcacggccgg acggggggcc gaggcccccc
     7081 gtccgccgat ccgttcgagg attcgtcccg ctaatccagg tcgccgaagc tcccgttctc
     7141 cagggtcgcc ttcacgacgc ccatgaaggc gtcggcctcg gccccgtcca cgagccggtg
     7201 gtcgaacgaa agggcgaggt accccatcgt ccggacggcg atcgtgtcgc tgccgtcggg
     7261 aagctcgatc accttcgccc gcttctcgat cgtcccgatg ttcaggatcg cgacctgcgg
     7321 ctggttgatg atcggcgtgc cgaagaggga gccgtagacc cccgggttcg tgatcgtgaa
     7381 cgtgccgccc tggacctcgt cggggaggag cttcttcgtc cgggcgcggc cggcgaggtc
     7441 gttcgcggcg cgggcgagcc cgacgatcga gaggtcgtcg gcgttcttga tgacggggac
     7501 gatgaggccc cagtcgagag cgacggccat gccgaggttg acggtgttga agaagaccat
     7561 cttgtcgtcg aagtagtggg cgttgcacat cgtgtggctg agggcgacgc gcgagacgac
     7621 gtagaggatg atgtcgttga gggggatgct gtcgggc
//
LOCUS       DAOTEZ010000189         7613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_6828_length_7613_cov_4.678883, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000189 DAOTEZ010000000
VERSION     DAOTEZ010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7613
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6828_length_7613_cov_4.678883"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2424
                     /locus_tag="P5164_11015"
     CDS             <1..2424
                     /locus_tag="P5164_11015"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRY44464.1"
                     /translation="VPLFEHRLFLTATPHNGFTRSFSGLLERLDPVRFTRRDELTPAE
                     EARVEDVLVRRLKREINERTNPPRFSERFLQPLPIRLSREEAALSEAFRSFRAALHSA
                     VASASRADQRAGSFAAEVLGKRLLSGPVPFADSWWRTVEGLAETTPADAAEVRAAERA
                     AGEETPNDAEAEARLAHAARTIGAWLLPLGEKLGAEIDAVGRALEALGLPAGGGTPAS
                     RRPRRDARFDALLALVRERLLENGRFRDDERLVVFTEYRTTLDSLAARLAEAFPAPGA
                     LRILTGGMPEAERDAIKAAFNDPDDPVRLLVATDAASEGLNLQETARYLLHYDVPWNP
                     ARLEQRNGRLDRYGQARDVTVFHFASDEDDDLGFLAYVIAKVESIREDLGSTGEVFEA
                     AFEAKFLEGRAAGEVRARVERGVAAARGRASYRRDARSRVAAKAAAAEQAEEEGRALE
                     ALAREVDLDPESLAATLESALAIGSVRPRLDPPDARGRRRLTPPIPASWSALADDALR
                     RPGAAGSRGALPALVFDPSAFVERKGGRPVFRPSADAVLLHLGHPVFQRVFSSWARAR
                     FPGSGLAASRWTVRRMPGSSLPDGAEAVLLLTVEELAVNELREAFHHWVRTLAWPVAK
                     GRLGPLLPHCPAREWRAGANLPTPADVAAARDLAGPLLDDLKAGVRAHAEALERGLSS
                     EVSRRGAEELEEERKRFVSRQGELSTLIEQQSVARLEKELARLREEARQGLLFAASSR
                     LEELERSIAAREEELRRRTLHYHELRDQLARERDRVLREIVPRRFALRGGVRVFPVAL
                     EVRLEANGG"
     gene            2427..2645
                     /locus_tag="P5164_11020"
     CDS             2427..2645
                     /locus_tag="P5164_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44465.1"
                     /translation="MATHANRPLDEKREHGLAEEFREAAADLLFMADLVEVGRDLDAV
                     DASLSACARSRKGAEQAGNALWAEEGEG"
     gene            2655..2885
                     /locus_tag="P5164_11025"
     CDS             2655..2885
                     /locus_tag="P5164_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017295742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRY44466.1"
                     /translation="MEVPSRAREFDVVIERDAEGWYVGSVPSLRGCHTQARSLDELHE
                     RIVEAIRLCLEVEDESAPPLEFVGIQRVRLAS"
     gene            2882..3106
                     /locus_tag="P5164_11030"
     CDS             2882..3106
                     /locus_tag="P5164_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016859790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HRY44467.1"
                     /translation="MTRLPRPTGRDLVGALHRAGFETVRVRGSHSFLRHPDGRTTVVP
                     VHAGQTIGPGLLLQVLRDCRLSVDELHTLL"
     gene            3143..>7613
                     /locus_tag="P5164_11035"
     CDS             3143..>7613
                     /locus_tag="P5164_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44468.1"
                     /translation="MSDASWWASLRHGGLLLSAAQVARLDAAPLAPFPSATAERVRRA
                     IVRAAADDLPPSARAAAQSDLLTLLLEEVAGLRDAARSAPRPASAPAAPAGHWLRGSD
                     VDASLTVRSVTGEAIRPRRLWLGGNGARLPVFFADEARLGVGRGKRAPARLLEWLRAR
                     SERLALLASPSQLRLAYAGADHDAFCEWDLALFFPEGEPGPQVEALRRLLAPQLLVPP
                     KPGEPAPLLRLVLDARKGQADLSSTLGERVRRAVELLVASHAPALAALEGRGQGLPHE
                     AVYRAAVRVVMRLVVLLFAEARPDLLPRENPVFHGSYSLAGLRESLERQGSGGGRERL
                     RQQAEGWPRVLALFRLLHAGSHHEALPVPRYGGRLFTPGDAASPDPQSRAVALLESAV
                     FDPALHGVMTDADVARLLDLLTRTKATMRQGTRTVTTTLPVDFESLSTEFIGILYEGL
                     LDYELRRAAPGDPVLFLDLGDQPALPLSRLSALSDAALAGLVEKLKAKVKPAGGEEDE
                     GDEGAEEAAEEDAEEEADAEEGAAAASEESDPLEAEVAVPADAGDTHLAAWETALAWA
                     RRAVVAGGLAPKPRGKKPEALAAWAAKAEGAARGLLREVVVPGGYYLVRWGGTRKGAG
                     TFYTRPQLAGPTARRTLHPLLYAEDGAPRRPEELLALKVCDPAMGSGSFLVSALRLLV
                     KALYASLHHHGRVATHGAGSRVVVTLPEGTPATGRLVEELLPCLPDDPDFEPRLLARL
                     KRHVVERCLYGVDLDPLAVELAQLSLWIETLDRSLPFEFLDHKLKAGNSLVGGWFDRY
                     RDYPLLAWEREGGDEKHDGVHHVKSVAPARGRAAGKEKTTGNLWGAAIREAYARAKAD
                     LGDLLDGQQGLFEQATGPDPAALHDEAVAAFDALHALPPHDPDGVAELYRERVLGSPS
                     LAALREAFDAWCALWFWPADRLDLAPLPSTFDRLAPEARAVVAEVAARERFFHWELEF
                     PDVFARPGSGFDAILGNPPWDTVQPNSKEFFSALDPLYRAYGKQEALKRQREIFEANA
                     ADEEAWLGYNGRFKAFASFAANAAAPFGDPAAGEGVSLHRSPSRSAERHGEWRSRRGK
                     VKGYADREHPFRQQGEGKPYTYKLFLEEAHALLRPGGRLGFLVPSGLYTDKGSIALRR
                     LFLERCRWTHLYAFQNERFLFEAVDHRFKALSLCVEKGGSTEAVLTRFRLGPGDSPEV
                     TELETDIPADERYLPVRADQIARLSPRSLALLEIRHPRDLEVLEKIYAGSVLLGDDSE
                     EGWGIKYAQGDFNMTSDSHLFAPRPEWEAKGYRPDEYGRWIGPEGEVALPLYEGRLFN
                     QFDFAAKEWVSGTGLSAVWSDVPWSAKVVRPQYLIAEAAARAWPKYVPAPKAAYRNIA
                     RSTDTRTFIATVLARDPTPHVTSLLLPRPSEGLAPEWMLCAAANSFVLDSVVRLRVGG
                     THLDYHVIAEFPLVRTRSSEVARVALRLGNPHARYAPEWLDLFQPAGTRAKSWR"
BASE COUNT          947 a         3046 c         2585 g         1035 t
ORIGIN      
        1 gttcccctct tcgagcaccg cctcttcctc acggcgacgc cgcacaacgg cttcacccgc
       61 tccttctcgg gccttctcga gcgcctcgac ccggtccgct tcacccggcg cgacgagctg
      121 accccggccg aggaggcgcg cgtcgaggac gtcctcgtcc gccggctcaa gcgcgagatc
      181 aacgagcgga ccaatccgcc ccgcttctcc gagcggttcc tccagcccct cccgatccgc
      241 ctctcgcgcg aggaggcggc cctctccgag gcgttccgca gctttcgcgc cgccctccac
      301 tcggccgtgg cctcggcctc gcgggccgac cagcgggcgg gctcgttcgc ggcggaggtc
      361 ctcggcaaga ggctcctttc gggtccggtc ccgttcgccg actcctggtg gcggacggtc
      421 gagggcctcg ccgagacgac gccggccgac gcggccgagg tccgcgctgc cgagcgggcg
      481 gcgggcgagg agaccccgaa cgacgccgag gccgaggcgc ggctcgccca cgcggcccga
      541 acgatcggcg cttggctcct gccgctgggc gagaagctcg gcgccgagat cgacgccgtc
      601 ggccgcgcgc tcgaagcgct ggggcttccc gccggcggcg gcacgcccgc gtcgcgccga
      661 ccgcgacgcg acgcgcgctt cgacgcgctc ctcgccctcg tccgcgagcg cctcctcgag
      721 aacggtcgat ttcgcgacga cgagcggctc gtcgtcttca ccgagtaccg gacgacgctc
      781 gactccctcg ccgcgcgcct cgccgaggcg ttccccgccc ccggcgcgct ccgaatcctc
      841 acgggcggga tgcccgaggc cgagcgcgac gccatcaagg cggccttcaa cgaccccgac
      901 gaccccgtcc ggctcctcgt cgcgaccgac gccgcctccg aggggctcaa cctccaggag
      961 acggcccgct acctcctcca ctacgacgtc ccctggaacc ccgcgcgcct cgagcagagg
     1021 aacgggcgcc tcgaccgcta cggccaggcc cgcgacgtca ccgtcttcca cttcgcgtcc
     1081 gacgaggacg acgacctcgg gttcctcgcc tacgtcatcg cgaaggtcga gtcgatccgc
     1141 gaggacctcg gctcgacggg cgaggtcttc gaggcggcgt tcgaggcgaa gttcctcgag
     1201 gggcgcgcgg ccggcgaggt gcgcgctcgc gtcgagcgcg gcgtcgccgc cgcccgcggc
     1261 cgggcctcct accgccgcga cgcgcgctcc cgcgtcgcgg cgaaggccgc cgcggcggag
     1321 caggcggagg aggaaggccg cgcgctcgag gccctcgccc gcgaggtcga cctcgacccg
     1381 gagtcgctcg ccgcgacgct cgagtcggcc ctcgccatcg gctccgtgcg cccgcggctc
     1441 gacccgcccg acgcgcgcgg gcggcggcgc ctgacgcctc cgatccccgc ctcctggtcc
     1501 gccctcgcgg acgacgccct tcgccgcccc ggcgcggccg gatcgcgcgg ggcgctcccg
     1561 gccctcgtct tcgacccgag cgcgttcgtc gagaggaagg gcgggcggcc ggtcttccgg
     1621 ccctcggccg atgccgtcct cctccacctc gggcacccgg ttttccagcg ggtcttttcg
     1681 agctgggcgc gggcgcgctt cccgggctcg ggcctcgcgg cctcccgctg gaccgtccgg
     1741 cgaatgccgg gatcctcgct cccggacggg gccgaggccg tcctcctcct caccgtcgag
     1801 gagctcgccg tcaacgagct gcgcgaggcg ttccaccact gggtgcggac gctcgcgtgg
     1861 cccgtcgcga aggggcgcct cggcccgctt ctcccgcact gcccggcccg cgagtggcgc
     1921 gccggagcca acctccccac gccggccgac gtcgccgcgg cgcgcgacct cgccggcccg
     1981 ctcctcgacg acctgaaagc gggcgtccgc gcgcacgccg aggcgctcga gcgcggcctc
     2041 tcgtcggagg tctcgcggcg aggcgccgag gagctggagg aggagcggaa gcggttcgtc
     2101 agccggcagg gcgagctctc gaccctcatc gagcagcaga gcgtcgcaag gctcgagaag
     2161 gagctcgccc gcctccgcga ggaggcgcgg cagggtctcc tcttcgccgc gtcctcccgc
     2221 ctggaggagc tcgagcgctc catcgccgca cgcgaggagg agcttcgccg ccggacgctc
     2281 cactaccacg agctgcgcga ccagctcgcc cgcgagagag accgcgtcct ccgcgagatc
     2341 gtcccgcgcc gcttcgccct ccgcggaggc gtgcgggtct tcccggtcgc gctcgaggtg
     2401 aggctggagg cgaacggagg atgagaatgg ccacacacgc gaatcgccct ctcgacgaga
     2461 agcgcgaaca cgggcttgcc gaagagttcc gtgaggcggc ggccgacctc ctgttcatgg
     2521 cggacctcgt ggaggtcgga agagacctcg atgcggtcga cgcgagcctt tcggcctgtg
     2581 ctcgctcccg caagggagcc gaacaagcgg ggaacgcgct atgggccgag gagggcgaag
     2641 gctagaatgg ccccatggaa gtcccctcgc gagcccgcga gttcgacgtg gtcatcgagc
     2701 gcgatgccga gggctggtac gtcggctccg tcccgtcgct ccgcggctgc cacacgcagg
     2761 ctcggtccct cgacgagctg cacgagcgga tcgtcgaggc gatccggctc tgcctcgagg
     2821 tggaggacga gtccgctccg ccgctcgagt tcgtcggcat ccagcgggtt cgcctcgctt
     2881 cgtgacgcgg ctgccgaggc cgacgggccg cgacctcgtc ggggcgctcc atcgggcagg
     2941 gttcgagacc gttcgcgtcc ggggaagcca ctcgtttctg cggcatcccg acggccgtac
     3001 gaccgtcgtt ccggtccatg cgggccagac gatcggtccc gggcttctgc tccaggtcct
     3061 gcgggactgc cggctctcgg tcgacgagct tcacacgctc ctgtagcgca gcctcggagg
     3121 aagcgatccg tcggaggcgc cgatgtctga cgcctcctgg tgggcctcgc tccgccacgg
     3181 cgggctcctt ctctcggccg cgcaggtggc gcggctcgac gcggcgccgc tcgccccgtt
     3241 cccctccgcg actgccgaga gggttcgccg cgcgatcgtc cgggccgccg cggacgacct
     3301 cccgccttcg gcgcgggcgg ccgcgcagtc cgacctcctg acgctcctcc tcgaggaggt
     3361 ggccggcctg cgcgacgcgg cccgctcggc gccgcgcccc gcctccgcgc cggccgcccc
     3421 cgcgggccac tggctccgcg ggtccgacgt cgacgcctcg ctcaccgtcc gctccgtcac
     3481 cggcgaggcg atccgccccc gccgcctctg gctcggaggg aacggcgcgc gcctccccgt
     3541 cttcttcgcc gacgaggccc gcctcggcgt cggccgcggc aagcgcgccc cggcccgcct
     3601 cctcgagtgg ctccgcgcgc gaagcgagcg gctcgccctc ctcgcctccc cgtcgcagct
     3661 ccgcctcgcc tacgcgggcg ccgaccacga cgccttctgc gagtgggacc tcgccctctt
     3721 cttcccggaa ggggagcccg gcccgcaggt cgaggcgctg cgccgcctcc tcgccccgca
     3781 gctcctcgtc ccgccgaagc cgggcgagcc ggcgcccctc ctccgcctcg tcctcgacgc
     3841 gcggaagggt caggccgacc tctcgtccac cctcggcgag cgcgtccgcc gcgccgtcga
     3901 gctcctcgtc gcgagccacg cgcccgccct cgccgcgctc gaggggcggg ggcagggcct
     3961 cccgcacgag gcggtctacc gcgctgccgt ccgcgtcgtc atgcgcctcg tcgtcctcct
     4021 cttcgccgag gcgcgtccgg acctcctccc gcgcgagaac cccgtctttc acgggtcgta
     4081 ctcgctcgcg ggcctgcgcg agagcctcga gcggcagggg agcggcggag gccgcgagcg
     4141 gctgcgccag caggccgaag gctggccgcg cgtcctcgcc ctcttccgcc tcctccacgc
     4201 cggctcgcac cacgaggcgc tccccgttcc ccgctacggc ggccgcctct tcacgccggg
     4261 cgacgccgcc tcgcccgacc cgcagtcgcg cgccgtcgcc ctcctcgaga gcgccgtctt
     4321 cgatcccgcc ctccacggcg tcatgaccga cgccgacgtc gcccgcctcc tcgacctcct
     4381 cacccggacg aaggcgacga tgaggcaggg gacgcggacc gtgacgacga ccctccccgt
     4441 cgacttcgag agcctctcca ccgagttcat cggcatcctc tacgaggggc tcctcgacta
     4501 cgagctccgc cgcgccgccc cgggcgaccc cgtcctcttc ctcgacctcg gcgaccagcc
     4561 cgcccttccg ctctcgcgcc tctcggccct ctcagacgcg gcccttgcgg gcctcgtcga
     4621 gaagctgaag gcgaaggtga agccggcggg gggcgaggag gacgaaggcg acgagggcgc
     4681 ggaggaggcg gcggaggagg acgccgagga agaggccgac gccgaggaag gggcggccgc
     4741 cgcgagcgag gagagtgatc cgctcgaagc ggaagtcgcc gttcccgccg acgccggcga
     4801 cacgcacctc gccgcgtggg agaccgcgct cgcctgggcg cgccgcgccg tcgtcgcggg
     4861 cggcctcgcc ccgaagccgc gcgggaagaa gcccgaggcc ctcgccgcct gggccgcgaa
     4921 ggccgaaggc gctgcgcgcg gcctccttcg cgaggtcgtc gtccccggcg ggtactacct
     4981 cgtccgctgg ggcggcaccc gcaagggcgc gggcaccttc tacacgcgcc cgcagctcgc
     5041 gggccccacg gcgcgaagga cgctccaccc cctcctctac gccgaggacg gcgcgccccg
     5101 ccgccccgag gagctcctcg ccctcaaggt ctgcgacccg gcgatgggct cgggctcgtt
     5161 cctcgtctcg gcgctccgcc tcctcgtcaa ggcgctctac gcctcgctcc accaccacgg
     5221 ccgcgtcgcc acgcacggcg ccggctcgcg cgtcgtcgtc acgctccccg aagggacgcc
     5281 cgccaccggc cgcctcgtcg aggagctgct gccctgcctc cccgacgacc ccgacttcga
     5341 gccccgcctc ctcgcgcgcc tcaagcgcca cgtcgtcgag cgctgcctct acggcgtcga
     5401 cctcgaccct cttgccgtcg agctggccca gctctccctc tggatcgaga cgctcgaccg
     5461 ctcgctcccg ttcgagttcc tcgaccacaa gctcaaggcc ggcaactccc tcgtcggagg
     5521 atggttcgac cggtaccgcg actacccgct cctcgcctgg gagcgcgagg ggggcgacga
     5581 gaagcacgac ggcgtccacc acgtgaagag cgtcgccccc gcccgcggcc gcgccgccgg
     5641 gaaggagaag acgaccggca acctctgggg tgccgcgatc agggaggcct acgcccgcgc
     5701 gaaggccgac ctcggcgacc tcctcgacgg ccagcagggg ctcttcgagc aggccactgg
     5761 ccccgacccc gcggccctcc acgacgaggc ggtcgccgcg ttcgacgccc tccacgccct
     5821 tccgccgcac gaccccgacg gcgtcgccga gctctaccgc gagagagtgc tcggcagccc
     5881 ctccctcgcc gccctccgcg aggcgttcga cgcctggtgc gccctctggt tctggcccgc
     5941 cgatcgcctc gacctcgccc cgctcccctc cacgttcgac cgcctcgcgc ccgaggcccg
     6001 cgccgtcgtc gcggaggtcg cggcgcgcga gaggttcttc cactgggagc tggagttccc
     6061 cgacgtcttc gcccgccccg gctccggctt cgacgcgatc ctcggcaacc cgccgtggga
     6121 caccgtccag ccgaacagca aggagttctt ctcggcgctc gacccgctct accgcgccta
     6181 cggcaagcag gaggcgttga agaggcagag ggagatcttc gaggcgaacg cggcggacga
     6241 ggaggcgtgg ctcggctaca acgggcggtt caaggcgttt gcgagcttcg cagcgaacgc
     6301 ggccgcgccg ttcggcgacc cggccgcggg ggagggcgtc tcccttcacc gctcgccgtc
     6361 gcgcagcgcg gagcggcacg gcgagtggcg gagccgacgc gggaaggtga agggctacgc
     6421 cgaccgcgag catccgttcc gccagcaggg tgagggaaag ccctacacct acaagctctt
     6481 cctcgaagag gcccacgccc tcctccgccc cggcggtcgc ctcggcttcc tcgtcccctc
     6541 ggggctctac acggacaagg gctccatcgc gcttcgcagg ctcttcctcg agcggtgccg
     6601 gtggacgcac ctctacgcct tccagaacga gcgcttcctc ttcgaggcgg tcgaccaccg
     6661 cttcaaggcc ctctcgctct gtgtcgagaa ggggggctcg acggaggcgg tcctcacgcg
     6721 cttccgcctc ggccccggcg actcgcccga ggtgacggag ctggagacgg acattccggc
     6781 cgacgagcgc tacctccccg tccgcgcgga ccagatcgcg cgcctcagcc ctcgctccct
     6841 cgccctcctc gagatccgcc acccgcgcga cctggaagtc ctcgagaaga tctacgccgg
     6901 ctccgtcctc ctcggcgacg actcggagga gggctggggg atcaagtacg cccagggcga
     6961 cttcaacatg acgagcgact cccacctctt cgccccgcgg ccggagtggg aggcgaaggg
     7021 ctaccggccg gacgagtacg ggaggtggat cgggccggag ggggaggtgg cgctgccgct
     7081 ctacgagggg cgtctcttca accagttcga cttcgcggcc aaggaatggg tgagcgggac
     7141 gggcctgagc gccgtctggt cggacgtgcc gtggtcggcc aaggtcgtac ggcctcaata
     7201 cctgatcgcg gaggcggccg cccgagcctg gccgaagtac gtgcccgccc cgaaggcggc
     7261 ctaccggaac atcgcccgaa gcacggacac acggacgttc attgcgacgg tattggctcg
     7321 agatccgact ccgcacgtga cctcgctgct cctgccccga ccttcggagg gtctcgctcc
     7381 ggagtggatg ctctgcgctg ccgccaacag cttcgtgctg gactccgtgg tccggcttcg
     7441 agtcggtggc acacatctcg actaccacgt catcgccgag ttcccgctcg ttcgcacgcg
     7501 ttcctccgag gtcgcccggg tggctctgcg gcttgggaat ccccatgcac gctacgcccc
     7561 ggagtggctc gacctctttc aacctgctgg gacacgagcg aagagctggc gcg
//
LOCUS       DAOTEZ010000190         7601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_6849_length_7601_cov_6.169891, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000190 DAOTEZ010000000
VERSION     DAOTEZ010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7601
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6849_length_7601_cov_6.169891"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="P5164_11040"
     CDS             complement(<1..1090)
                     /locus_tag="P5164_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRY44469.1"
                     /translation="MIIDKALSKIFGTKHDRDMKALTPLVEEIGRLEPELAALPLDGL
                     RSRLGEIRERVESELKDLPSDLEARRAKTRSVLDPFLPRVFALVREAGKRTIGQRHYD
                     VQLRGGAVLHQGKIAEMRTGEGKTLVATLPVTLNAIAGRGAHVVTVNDYLARRDAEWM
                     GRIYTALGLTVGCIQHDLDDAARREAYACDITYGTNNEFGFDYLRDNMKFELTQMVQR
                     PHFFALVDEVDSILIDEARTPLIISGPSEGDVDIYYRCDRIVPKLVRGEEIKDKYGNK
                     TTTGDYLVDEKAHTAALTEEGVGKCERLLGVENLYDPTNIDVLHACQQALRAHTLYKR
                     DVNYVVKDGQVIIVDEFTGRTMPGRRWSD"
     gene            complement(1176..2054)
                     /locus_tag="P5164_11045"
     CDS             complement(1176..2054)
                     /locus_tag="P5164_11045"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRY44470.1"
                     /translation="MTRKQHTIIVVPHARARFRQFQVTSRLLWSVATAVSLSLLLGVV
                     FGVLWLQSVRKNREVTSLVAENQDLRGRTKSLNAKLESLEKVLADFEERTRRLSIVAG
                     LSGSPDPGTGGVGGLTALPADDARHTEAVLEEASRRGLLLSGQLGEVESRLSLQADQL
                     ALTPTLAPSLGVLTAGFGLRADPFTGRPDFHAGIDISSPKGSRIVAPASGTVVRVGWD
                     RGYGRVVEIAHGFGIRTLYGHLEMPRVAEGQRVRRGDVVGLVGSTGRSTGPHLHYEVQ
                     VAGKPVNPLDYVLNAF"
     gene            complement(2194..3720)
                     /locus_tag="P5164_11050"
     CDS             complement(2194..3720)
                     /locus_tag="P5164_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRY44471.1"
                     /translation="MSVARAWSAALVGLEARPVVVEAALGLGLPGLTIVGLPDAAVRE
                     SRERIRSALRQIGLPLPGRSVVVNLAPADLPKAGTLYDLAVALAVLAANGDVPLEGLA
                     ELTLAGELALDGEVRPVPGVLPIAESARAEGRSQLVVAPQNAAEAASVPGVIVLPAPH
                     LGAVVAHVAGRELLKPFVPAEEPAAPEADAPDLVEVRGQAIARRALEIAAAGGHNLLL
                     FGPPGSGKTMLARRLPGILPPLSPEESLETTRIWSAAGRLPAGSGLLRTRPFRSPHHG
                     ASAPALAGGGPDPRPGELSLAHHGVLFLDELPEFRRDALEALREPLEEGIVRVTRASG
                     SRSFPARFLLVAAMNPCPCGFRGDPRKECTCSEHDLGRYRRKVSGPLLDRIDLHVEVP
                     ALPSADLVADVPPEPSSLVRARVLAARERQAERTGDRRLTNAALTPGRLRATGAVLPE
                     AAALLSRAMDRLGLSARGHQRVLRVARTIADLEGSPSTGPAHVAEALRYRAAAPDRTR
                     "
     gene            complement(3731..5608)
                     /locus_tag="P5164_11055"
     CDS             complement(3731..5608)
                     /locus_tag="P5164_11055"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRY44472.1"
                     /translation="MATETTERARTRAANRGRALAGGILASLLAAGCSSAPAPAPATN
                     VPVVAAAAEATCPPADPRQEASDRFHRGKAHALAGDASCARLEFQAALDGFRESMRPG
                     NAGDLAFAGQLWESVRLYESIPSEDEEEPVAGDRDGLVAREEAAPTADELAVARREIE
                     EAGASVSFDVPIVVNEAVLDAVAYYQFRTPKAFAAALQRSGRYLPLMRSILRENGIPE
                     DIVYLAMIESAFKSQAHSRAAAKGYWQFIAGTGKRYGLRTTRDLDERSDPVKSTRAAA
                     AYLRDLYEMFGDWYLAMAAYNAGEGRVLRGLQRTGARDYWELREARALHRETSDYVPF
                     FIASALIAKDPARFGFDVVPDPPLEFDVVEVPRPVALARVAREIGTTVEHLRSLNGEL
                     RRATTPHGAARYPLRVPKGAGAILQARLESIPAAPDVRERRLKVRKGDTLARFAKRHG
                     VHVADLREANRLRPNAKLHAGQLLVVPGRFEAPRTPPAELAEAADAPVLPEMERGEIR
                     ALPTPSAAVVDAASLGADLPGIAVTPLPSAPHPAVVEIPRDGFETEHVAPHPSENRAS
                     ARTKPAARRTRHTVRRGETLFRIASRYGVTVDALRRANRIGRGHVIRPGQRLAVPTAT
                     R"
     gene            complement(6358..6936)
                     /locus_tag="P5164_11060"
                     /pseudo
     CDS             complement(6358..6936)
                     /locus_tag="P5164_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
     gene            complement(6964..>7601)
                     /locus_tag="P5164_11065"
     CDS             complement(6964..>7601)
                     /locus_tag="P5164_11065"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HRY44473.1"
                     /translation="PLAANLAAVLPAAAAGAAAVHLSGALGPEVLAPLAARGFATAAC
                     HPLQTFTGSPEDATRLGGAAFAVEGSQEGRTAAEAVVRALGGRPFPLPSGARPLYHLA
                     ASLGANGLTGLVAAARDALSATGIGPDAALSALAPLLRAALHEALRSGPEAALTGPVS
                     RGDEATLERHRRALHAWDESRVALFEALLDEQRRLAGRCVGAADAKMPRSD"
BASE COUNT         1000 a         2554 c         2977 g         1070 t
ORIGIN      
        1 cgtcggacca gcgccggccc ggcatggtgc ggccggtgaa ctcgtcgacg atgatgacct
       61 gcccgtcctt cacgacgtag ttcacgtcgc gcttgtagag ggtgtgggcg cggagagcct
      121 gctggcaggc gtggaggacg tcgatgttcg tcgggtcgta gaggttctcg acgccgagaa
      181 gccgctcgca cttcccgact ccctcctcgg tgagcgcggc cgtgtgcgcc ttctcgtcga
      241 cgaggtagtc gcccgtcgtc gtcttgttgc cgtacttgtc cttgatctcc tcgccgcgga
      301 cgagcttcgg gacgatccgg tcgcaccggt agtagatgtc gacgtcccct tccgacgggc
      361 ccgagatgat gaggggggtc cgggcctcgt cgatgaggat cgagtcgacc tcgtcgacga
      421 gggcgaagaa gtgcggccgc tggaccatct gcgtgagctc gaacttcatg ttgtcgcgca
      481 ggtagtcgaa gccgaactcg ttgttcgtcc cgtacgtgat gtcgcaggcg tacgcctcgc
      541 ggcgcgcggc gtcgtcgagg tcgtgctgga tgcagccgac ggtcaggccg agggcggtgt
      601 agatccgtcc catccactcc gcgtcgcgcc gggcgaggta gtcgttcacg gtgacgacgt
      661 gcgcgcctct cccggcgatc gcgttgaggg tgacggggag cgtcgccacg agagtcttcc
      721 cctcgccggt ccgcatctcg gcgatctttc cctggtggag cacggccccg ccgcgcagct
      781 ggacgtcgta gtggcgctgg ccgatcgtcc gcttgcccgc ctcgcggacg agggcgaaga
      841 ccctcgggag gaacgggtcg aggaccgacc gggtcttcgc ccggcgggcc tcgaggtcgg
      901 aggggaggtc tttcagctcg gactcgaccc gctcccggat ctcgccgagg cgggaccgga
      961 ggccgtccag ggggagcgcg gcgagctcgg gctccagccg gccgatctcc tcgacgagcg
     1021 gcgtgagcgc cttcatgtcg cggtcgtgct tcgtcccgaa gatcttggag agtgccttgt
     1081 cgatgatcat cttccgtcca tgggcgcccg gcgggggcgc aagctccgga tcctagcaga
     1141 ggggctcggg gtcgcccgcc gtcgcgcgga tcggctcaga acgcgttcag gacgtagtcg
     1201 agcgggttga ccggcttccc ggcgacctgc acctcgtagt ggaggtgcgg cccggtcgac
     1261 cgcccggtgg agccgacgag gccgacgacg tctccccggc ggacgcgctg gccctcggcc
     1321 acccgcggca tctcgagatg gccgtagagg gtccggattc cgaagccgtg ggcgatctcg
     1381 acgacacggc cgtagccccg gtcccagccg acccgcacca ccgttccgct cgcgggggcc
     1441 acgatccggc tgcccttcgg cgaggagatg tcgatgccgg cgtggaagtc cggtcgtccc
     1501 gtgaacgggt cggcccggag cccgaagccg gcggtcagga ccccgagcga cggcgcgagc
     1561 gtcggcgtga gcgcgagctg gtcggcctgg agcgacagcc ggctctcgac ctccccgagc
     1621 tgccccgata ggaggagccc gcggcgggag gcctcctcga ggaccgcctc cgtatggcgg
     1681 gcgtcgtcgg ccgggagcgc cgtcaggccg ccgacgcctc ccgttcccgg gtcgggggag
     1741 ccggagaggc cggcgacgat ggagagacgg cgggtccgtt cctcgaagtc ggcgaggacc
     1801 ttctccagcg actcgagctt cgcgttcagg ctcttcgtac ggccgcggag gtcctggttc
     1861 tcggcgacga ggctcgtcac ctcgcggttc ttccggaccg actggagcca gaggacgccg
     1921 aagacgaccc cgaggagaag cgaaagggat acggccgtcg cgacactcca gaggagccgg
     1981 gaggtcacct ggaactggcg gaatctcgcg cgcgcgtgcg gtacgacgat gatcgtgtgc
     2041 tgctttcgcg tcaacggaat ctccgggacc gccgggacgg gtcgtccccc caggctttgt
     2101 gacgagccgc acggaagtct atcgacccgt cgtctcccgt caagcgaaag ttttttggaa
     2161 cgaaatcgtt gagcccgcga gacttgccgc gaatcagcgg gtccggtccg gtgccgcggc
     2221 ccggtagcgg agcgcttcgg cgacgtgggc cggtccggtc gagggggagc cctcgaggtc
     2281 cgcgatcgtc ctcgcgacgc gaaggacccg ctggtgaccg cgtgccgaaa ggcccagccg
     2341 gtccatcgcg cgcgagagga gagcggcggc ctccggcagg acggcgccgg tcgcccgcag
     2401 gcgcccgggc gtgagcgccg cgttcgtcag gcgccggtcg ccggtccgct ccgcctgacg
     2461 ttcccgcgct gcgaggacgc gggcccggac gagcgaggac ggctcgggcg ggacgtccgc
     2521 caccaggtcg gcggacggga gcgccgggac ctcgacgtgg aggtcgatcc ggtcgaggag
     2581 cggacccgag accttccgcc gatagcggcc caggtcgtgc tcggaacaag tgcactcttt
     2641 tcggggatct ccacggaacc cgcacggaca cgggttcatc gcggccacga ggaggaagcg
     2701 cgcggggaag gagcggctgc cgctcgcgcg ggtcacgcgg acgatgccct cctcgagggg
     2761 ctcgcggagc gcctcgagcg cgtcccggcg aaactccggc agctcgtcga ggaagaggac
     2821 gccgtggtgg gcgagcgaga gctcgccggg tctggggtcc gggccgcccc ccgccagggc
     2881 gggcgccgac gcaccgtggt gcggagagcg gaacgggcgt gtccgcagca gtcccgatcc
     2941 ggccggaagg cgcccggccg cgctccagat ccgcgtcgtc tccagcgact cctccggcga
     3001 gaggggaggg agaatccccg ggagccggcg ggcgagcatg gtcttgccgg agcccggtgg
     3061 gccgaacagg aggaggttgt gcccgcccgc ggccgcgatc tcgagggctc tgcgggcgat
     3121 cgcctggccg cggacctcga cgaggtcggg agcgtccgcc tccggcgcgg cgggctcctc
     3181 cgccgggacg aaaggcttca ggagctcgcg gccggcgacg tgcgcgacca ccgcgccgag
     3241 gtggggcgca gggaggacga tcacgccggg gaccgaggcc gcttcggccg cgttctgcgg
     3301 cgcgacgacg agctgggacc gcccctcggc gcgggccgac tccgcgatcg ggaggacgcc
     3361 gggaacgggc cgcacctcgc cgtccagggc gagctcgcct gcgagcgtca gctcggcgag
     3421 cccctcgaga gggacgtctc cgttggcggc gagaacggcg agggcgacgg cgaggtcgta
     3481 gagcgtcccc gccttcggga ggtcggcggg agcgaggttg acgacgacgc ttctgccggg
     3541 gagcggcagg ccgatctgcc ggagcgcgga gcgtatccgc tcccggcttt cgcggacggc
     3601 ggcgtccggg aggccgacga tcgtgaggcc gggcagcccc agcccgagcg cggcctcgac
     3661 gacgacgggg cgcgcctcga gcccgacgag ggcggcgctc caggccctcg cgacgctcat
     3721 ctcgcccggg tcagcgcgtg gccgtgggga cggcgagcct ctggccgggc cggatcacat
     3781 ggccccggcc gatccggttt gcccggcgaa gcgcgtcgac ggtgacgccg tagcggctcg
     3841 cgatccggaa gagcgtctcg cccctgcgga cggtgtgcct cgtccgtcgg gccgccggct
     3901 tcgtcctcgc ggaggctcgg ttctcggacg ggtgcggcgc gacgtgctcc gtctcgaagc
     3961 cgtcccgcgg gatctccacg acggccgggt gcggggcgga cggaaggggc gtcacggcga
     4021 tcccggggag gtcggctccg agggatgcgg cgtcgacgac ggccgccgac ggcgtcggga
     4081 gggcgcggat ctcgccgcgc tccatctcgg ggaggaccgg cgcgtcggcg gcttcggcga
     4141 gctcggccgg cggggtgcgg ggcgcctcga accgccccgg cacgacgagg agctgcccgg
     4201 cgtgaagctt cgcgttcggg cggaggcggt tcgcctcgcg gaggtcggcg acgtggacgc
     4261 cgtgccgttt cgcgaaccgg gccagggtgt cccccttccg gaccttcagg cgccgttccc
     4321 ggacgtccgg ggcggcgggg atgctctcga gccgggcctg gaggatcgcg ccggccccct
     4381 tcgggacgcg gagcggatag cgggcggcgc cgtgcggcgt cgtcgcgcgg cggagctcgc
     4441 cgttgagcga ccggaggtgc tcgacggtcg tcccgatctc gcgagcgacg cgcgcgaggg
     4501 cgaccggacg cgggacctcg acgacgtcga actccagggg ggggtcgggg acgacgtcga
     4561 agccgaaacg agccgggtcc ttcgcgatga gggccgacgc gatgaagaac gggacgtagt
     4621 cgctcgtctc gcggtggagc gcgcgggcct cgcgaagctc ccagtagtcc cgcgcgcccg
     4681 tccgctgaag gccgcgcagg acccggcctt ctcccgcgtt gtaggcagcc atcgcgagat
     4741 accagtcccc gaacatctcg tacaggtcgc ggagataggc ggcggcggcg cgcgtcgact
     4801 tgaccgggtc gctcctctcg tcgaggtccc gggtcgtccg gaggccgtac cgcttcccgg
     4861 tgccggcgat gaactgccag taccccttcg ccgccgcgcg cgaatgggcc tgcgacttga
     4921 aggcggactc gatcatcgcg aggtagacga tgtcctcggg aatgccgttc tcgcgcagga
     4981 tggaccgcat caggggaaga tagcgtccgc tcctctggag ggccgcggcg aacgccttcg
     5041 gcgtgcggaa ctggtagtag gcgacggcgt ccaggaccgc ctcgttcacg acgatgggga
     5101 cgtcgaagga gacggacgcg cccgcctcct cgatctcgcg gcgggcgacg gcgagctcgt
     5161 cggccgtggg ggccgcctcc tcgcgggcga cgagcccgtc gcggtcgccg gcgacgggct
     5221 cctcttcgtc ctccgagggg atcgactcgt agagccggac gctctcccag agctggccgg
     5281 cgaaggccag gtccccggcg tttcccgggc gcatcgactc gcggaacccg tcgagggcgg
     5341 cctggaactc gaggcgggcg caggatgcgt ccccggccag cgcgtgcgcc tttccccggt
     5401 gaaagcgatc gcttgcttcc tgccgtgggt cggcgggggg acaggtggcc tcggcggcgg
     5461 ccgcgacgac cggcacgttc gtcgccgggg cgggagcggg cgccgaggag catccggcgg
     5521 cgaggagcga agcgaggatg ccaccggcca gggcgcgccc ccggttcgcg gctcgggtgc
     5581 gggccctttc ggtcgtctct gtcgccactg tccgcgtcac tccgcctgga cgttgaaccg
     5641 gagggagagc aggagcttct ggaggtcggc cgggctcgag aggtcgatgg agaagctgct
     5701 gtccgtaggc agcgggcttc cggctccgtc ggagaacttc agctcgatcg tcacttgtcg
     5761 gctccttccc agcacctggc gcgggacagt gaggtcgaag gaccgagtga cgtccttctt
     5821 gtggttgagg ttcgaggcga ggaggtcgtc gatggagggg ccgccgccct ttcggccggc
     5881 gtcggggcgg gccgtcgcgc cggcggccgc cttctgcgtc gagagcttcc ggagcttgtc
     5941 gagctcggag aggatgtcga cgctcgagcg gaccttcacg tgcttgacgg ccggctcgtc
     6001 ggaggccggg gcggggccgg ccgactcgcc gaagctgatc tccgcggccg gctcctcggc
     6061 cggtcggtcg gcggacgccg gcgcggcggc cggctcgggc gcggccgctg cagggagcgg
     6121 ctcgacgctg ccttcggggc cccgggcctc gggcaggggc ggcgtcgccg ccccgctccg
     6181 ggccagctcg gagaaccggg cgaggcggtc gagcgtggcc gaggacgagg gggccgtccg
     6241 gaccgtggcg cccggaggcg gcggcggtac ggccgcttcc gtcggaacca ccggagcggc
     6301 cggcgtcgcg gggctggcga agaccgtggg gcgccgggct tcgccggtca ttctcgagcg
     6361 gagggccctg agggtcagct tcgagatccc cttgagcgtc tcgaagaccc ccgtgccgtt
     6421 cgctgcggag gcctcgaaga agtcgatctt cccgtccggg ttcagcgcgg cgttgagctc
     6481 gtcgacggtc aggatgttgg agaggtcgcg cttgttgaac tggaggacga gggggatctc
     6541 gccgacctcc agaccgatct cggcgaggtt ctcccggagg ttccggaagc tctccttgtt
     6601 cggctcgagc atcggccgct gggaatcggc cacgaagacg atcccgtcga cgcccttcag
     6661 gacgagcttc cgggtcgtgt tgtagaagac ctgcccgggg accgtgtaga gctggaggcg
     6721 cgtcttgaag cctccgatga ccccgacgtc gatcgggagg aggtcgaaga agagggtccg
     6781 gtcggtctcc gtctcgagcg agaccatctc gccgcgggcc gtcggcgccg tccgtccgta
     6841 gatgacgtgg aggttggtcg tcttcccgca caggccagga ccgtagtaga cgatcttggc
     6901 ggccatctgc atggtggcgt agttgaagaa aaccatctct ccgtcgcggg cccggcggcc
     6961 tcctcagtca gatcggggca ttttagcatc ggcagcgccg acgcaccggc cggcgagccg
     7021 ccgctgctcg tcgagcagcg cctcgaagag cgcgacccgg ctctcgtccc acgcgtgaag
     7081 cgcgcggcgg tgccgctcga gcgtggcctc gtccccgcgc gagacgggcc ccgtcagggc
     7141 ggcctcgggc ccggagcgaa gcgcctcgtg cagggcggcc cgcagcagcg gcgcgagcgc
     7201 ggagagcgcg gcgtcggggc cgatgccggt cgccgagagg gcgtcgcgcg ccgccgcgac
     7261 gagccccgtc aggccgttcg ccccgaggga agcggcgagg tggtagagcg ggcgggcgcc
     7321 ggacggaagc gggaacggac gccctccgag ggcgcggacg acggcttccg cagccgtgcg
     7381 tccctcctga gatccttcga cggcgaatgc ggcgcctccg aggcgggtcg catcctccgg
     7441 ggagcccgtg aacgtctgga ggggatggca ggccgccgtg gcgaaaccgc gcgcggcgag
     7501 gggggcgagg acctccgggc cgagcgcccc tgagaggtgc acggcggccg ccccggccgc
     7561 ggccgcgggg aggacggcgg ccaggttcgc ggcgagaggc g
//
LOCUS       DAOTEZ010000191         7579 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_6878_length_7579_cov_7.879054, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000191 DAOTEZ010000000
VERSION     DAOTEZ010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7579
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6878_length_7579_cov_7.879054"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="P5164_11070"
     CDS             <1..339
                     /locus_tag="P5164_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44474.1"
                     /translation="NWVATLAPRIAGDEEVRLKPVGIVPSVSEGKSGLLVAWLSAGPG
                     DESRDTPHRDRGRPFLARYDIEGEKPKSWVEIEDGTRIGELTSSPDGQAFAVTYRDMS
                     KEWSISRVDF"
     gene            complement(572..4108)
                     /gene="smc"
                     /locus_tag="P5164_11075"
     CDS             complement(572..4108)
                     /gene="smc"
                     /locus_tag="P5164_11075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRY44475.1"
                     /translation="MFRLKKLEIHGFKSFVDRTSLTLPSRLTAIVGPNGSGKSNICDA
                     VMWVLGEQSARALRGTTMEDVIFNGSARRRPLGMAEVSLTLESRGGQWADTGGEVVLT
                     RRVTRDGTSDYILNGRKARLKDIQDLLLGTGLGVRAYAIIEQQKIDLILSAKPQDRRR
                     LIEEAAGISKYKIRKQQAEVKLEETRANLLRLTDIVSEIERACTSLKRQASRAARWKE
                     QADVLSGLKKRLLRLKSERLVAATEAAEAALKSTTDAEGAALAESGRLDAAFEEARHA
                     AEEAERLRRERADAFAAAREALLAAEAAVAAAAREAEETEARRELVGSQRTEAEEDHL
                     SSVEEATKAEQLLAEAAEELAAAEARAKETGEALAAATRDESDATTRREGSRRELLAA
                     AARATEATNREREAALLVDRLGFALTKVEERKGRAELQLQERRSALEAARDLEAETNE
                     RLAEARRRLEEAGVSRKSAEERLASLKEERERATRQFHALDKRLGALETLLAAREAGG
                     EAARRALETSGAKADGVLADHFDAEEGFEHALDAALGGALEAPVVRDRAALASLIDTV
                     RASHLGTARFVHPIPETPFPLKPADARVLGLARDLLTARPGDEGLSAALPDALVVASV
                     DDALSLAELLPERTFVTKDGVVVRGSIVEAAGTEVPGEGLFTVRRDLKNVGAEKDALS
                     ERLDALEEEISGTGAQAGEAAQAVEREIAASRRAEREHSEALARLSAARDEATRAERE
                     VATILEEEKQLRADLLRVGEERAKALELSAQKTGVMTAMEAAIAGLESGLESARAVRL
                     SAQQADGEARAARDVLRERRRAAQEAFQTHRNRADAAARRLAELSQQITALGERKERA
                     LGAGEEARERREEAVVAASEAEAALKQADAAAVQRRGGLEELDRGAHAAREALDAARR
                     ARFEAELVVERRRGDREHLSETCRAEFGVPPEELPPVERAEGEPEPDDEALAAMQEEV
                     VQKTVALERLGPVNHAALEEFDVESKRLQEMSTQKLDLEKSLDQILETIRTINLTSSE
                     RFTQAFVAINANFAQVFQKLFGGGTASMQLLDESDPLDSGIEIMAQPPGKRNQTIGLL
                     SGGEKALTAVSLLVSIFRYKPSPFCILDEVDAPLDEANIDRFTTLLNELSEETQFVLI
                     THSKRTMETAQALYGVTQEEPGVSKIVSVRFD"
     gene            complement(4183..5151)
                     /locus_tag="P5164_11080"
     CDS             complement(4183..5151)
                     /locus_tag="P5164_11080"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRY44476.1"
                     /translation="MPRSLIDAVPFSAPRDADLEALVATLGAALAPDAFELVLLCPSS
                     GRRFRRRLAGGRDSGLRLAPASRNGGLPNGNVLTVPFGEEGGWRGRLYLVRRRFRPWT
                     GAESMRFSLLAPLACQLLTRLAEAPVARCAQERLLALAEATEAPAMILDRGGTILYAN
                     GAAEELLSREAEEGPTVLSGDRRAIPLVPHLLQLAGREAPATERIALTDGRCLEARIV
                     DCPGDGTSVAPPARIVLLKERAAPTIDDVRPHLAARGVSDREAEVVSGVLRGLRNSEI
                     AGELFISEYTVKDHLKHAFRKLSVASRGELLRALHAVPGRPPARVA"
     gene            5287..7209
                     /locus_tag="P5164_11085"
     CDS             5287..7209
                     /locus_tag="P5164_11085"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRY44477.1"
                     /translation="MLSFEVRKPGGESEIRKVHRDVIHLGASSGNDLVVRARGVLGRH
                     ARISVAGEELRLDVLGTAPGSDVYLNGAIVKSAALAAGDRIQIGEATITLLNGPAPNP
                     NRIAAPPPRGRDAFSVASAALSPLPPAPAPPAPSAPRAPEPRPRSYEPVHAVRPAENR
                     SLSAAQGFRLADVWGEIFGRLRASATLEERLQEVASYLSSSTPVHSVAFLSVADSSTG
                     EAIAAIWKGTLPRLGRRSVDEVFAAGGPTDVFEAGMRLRVYPVTRPGAEAVGLLVLPM
                     ELAADPLLNDLAVALAAAIGFVHAERADAPFGERASAHAPAAMVTAPLEDPVTAVVGS
                     SLAIQNLRVSLQRIASARAPVLVIGDVGTGKTLVAETLHALSPRRPRPLVRISATAST
                     AAALEEDLLGTAGPPDRRRRPGRLFEADGGTLLIEEVSELPAPTQALLYRLLEAREVV
                     APGGRSVPVDVRVIGTSSRPLTRAVEEGTFRDDLYYRLSALTLRLPTLRDRKEDIPAL
                     LDAFAARHGGPPAAAFDVEAMNALLDFNYAGNVREVENEVRRLAAQLGAGPVRLSDLD
                     AKFGDEHVELTLAESDDLKEIVEKVERQVIERVMRKVRGNQSLGARLLNISRGSLIAK
                     MKEYDIKDFRYLKRQD"
BASE COUNT          941 a         2732 c         2812 g         1094 t
ORIGIN      
        1 aactgggtgg cgacgttggc tccgcgaatt gcgggcgacg aggaggtgcg cctcaagccg
       61 gtcggcatcg tcccgtccgt ctccgaaggc aagagcggcc tgctcgtcgc atggctcagt
      121 gcggggccgg gggatgagag ccgggatacg ccccaccggg accgcgggcg tccctttctc
      181 gctcgctacg acatcgaagg cgagaagccg aagagctggg tcgagatcga ggatggtacg
      241 cggatcggcg agctgacttc gagccccgat ggccaggcct tcgctgtcac ctaccgtgac
      301 atgtcgaaag agtggtcgat ctcgagagtc gacttctaga ccgaatacgc cattgggcgg
      361 acggttgccg tagaggccgg ccacccatgg cggcctcagg atcccaggtc tctctcgtca
      421 gtcgaagcga acgctcacga tcgctcgccg gggggctggc tcgccggggg gctgagcttc
      481 gcgcgccccc cggacccccg cccaggaacg gatgtcctcg gcaaccgcga gagagcgtgc
      541 gcgcgccccc cggacccccg cccgatcctc ttcagtcgaa gcgaacgctc acgattttgc
      601 tgacgcccgg ctcttcctgc gtgacgccgt agagcgcctg ggcggtctcc atcgtcctct
      661 tcgagtgagt gatgaggacg aactgcgtct cctcggagag ctcgttgagg agcgtcgtga
      721 agcggtcgat gttcgcctcg tcgagcgggg cgtcgacctc gtcgaggatg cagaacggcg
      781 acggcttgta gcggaagatc gagacgagga gcgagacggc ggtgagcgcc ttctcgccgc
      841 ccgagaggag cccgatcgtc tggttccgct tgccgggggg ctgggccatg atctcgatcc
      901 ccgagtcgag cgggtcgctc tcgtcgagga gctgcatcga ggcggtgccg ccgccgaaga
      961 gcttctggaa gacctgcgcg aagttcgcgt tgatcgccac gaaggcctgc gtgaagcgct
     1021 cggaggaggt gaggttgatc gtccggatcg tctcgaggat ctggtcgagc gacttctcga
     1081 ggtcgagctt ctgcgtcgac atctcctgga gcctcttgct ctcgacgtcg aactcctcga
     1141 gcgcggcgtg gttgaccggg ccgagccgct cgagggcgac cgtcttctgg acgacctctt
     1201 cctgcatcgc ggcgagggcc tcgtcgtcgg gctcgggctc gccctccgcc cgctccaccg
     1261 gcgggagctc ctccggcggg acgccgaact cggcgcggca ggtctccgag aggtgctcgc
     1321 ggtcgccgcg gcgccgctcg acgacgagct cggcctcgaa gcgggcgcgg cgcgcggcgt
     1381 cgagcgcctc gcgggccgcg tgggcgcccc ggtcgagctc ctcgaggccg ccgcggcgct
     1441 gcaccgcggc cgcgtccgcc tgcttcaggg cggcctcggc ctcggaggcg gcgacgaccg
     1501 cctcctcgcg ccgctcgcgc gcctcctcgc cggctccgag ggcgcgctcc tttcgctcgc
     1561 ccagcgcggt gatctgctgg gagagctcgg cgaggcggcg ggcggcggcg tcggcccggt
     1621 tccggtgggt ctggaacgcc tcctgcgccg cccgccgccg ctcgcggagg acgtcgcgcg
     1681 cggcgcgcgc ctcgccgtcg gcctgctggg ccgagaggcg gacggcgcgg gccgactcga
     1741 ggccgctctc gaggccggcg atcgcggcct ccatggcggt catcacgccg gtcttctgcg
     1801 cggagagctc gagcgccttc gcgcgctcct cgccgacgcg caggaggtcg gcgcggagct
     1861 gcttctcctc ctccaggatc gtcgcgacct cgcgttcggc gcgggtcgcc tcgtcgcggg
     1921 cggcggagag gcgcgcgagc gcctcggagt gctcccgctc ggcgcggcga gacgcggcga
     1981 tctcgcgttc gacggcctgg gccgcctcgc cggcctgcgc ccccgtcccg gagatctcct
     2041 cctcgagggc gtcgaggcgc tcggagaggg cgtccttctc ggcgccgacg ttcttcaggt
     2101 cgcgccggac ggtgaagagc ccctcgcccg gcacctcggt gccggcggcc tcgacgatcg
     2161 agccgcggac gacgaccccg tccttcgtga cgaacgtccg ctccggcagg agctcggcga
     2221 gcgaaagggc gtcgtcgacg gaggcgacga cgagggcgtc ggggagggcg gccgagaggc
     2281 cctcgtcgcc gggccgcgcc gtcaggaggt cgcgggcgag gccgaggacc cgcgcgtcgg
     2341 ccggcttgag ggggaacggc gtctcgggga tcgggtggac gaagcgcgcc gtgccgaggt
     2401 gcgaggcgcg gaccgtgtcg atgagcgagg cgagcgccgc gcggtcgcgg acgaccgggg
     2461 cctcgagggc gccgccgagc gcggcgtcga gcgcgtgctc gaacccctcc tcggcgtcga
     2521 agtggtcggc gaggacgccg tcggccttcg cgccggaggt ctcgagggcg cgccgcgccg
     2581 cctcgccgcc cgcctcgcgc gccgcgagga gggtctcgag ggcgccgagg cgcttgtcga
     2641 gggcgtggaa ctggcgcgtt gcgcgttcgc gctcctcctt gagggaggcg agccgctcct
     2701 cggcggactt cctggagacg cccgcttcct cgagccgccg ccgggcctcc gcgaggcgct
     2761 cgttcgtctc ggcctcgagg tcgcgcgccg cttccaacgc ggagcggcgt tcctggagct
     2821 gaagctccgc gcgccccttg cgctcctcga ccttcgtcag ggcgaagccg aggcggtcga
     2881 cgaggagcgc ggcctcgcgc tcccggttcg tcgcctcggt ggcgcgggcg gcggcggcga
     2941 ggagctcccg ccgggagccc tctctccgcg tggtggcgtc gctctcgtca cgggtcgcgg
     3001 cggcgagcgc ctcgcccgtc tccttcgccc tcgcttcggc ggccgcgagc tcctcggcgg
     3061 cctccgccag gagctgctcg gccttcgtcg cctcctcgac ggaagagagg tggtcctcct
     3121 ccgcctcggt gcgctgcgag ccgacgagct ccctgcgggc ctcggtctcc tcggcctcgc
     3181 gggcggccgc ggcgaccgcg gcttcggcgg cgaggagcgc ctcgcgggcg gcggcgaagg
     3241 cgtcggcccg ctcgcgccgg aggcgttcgg cctcctcggc ggcgtggcgc gcctcctcga
     3301 aggcggcgtc gaggcgcccc gactcggcga gagcggcccc ttccgcgtcg gtcgtcgact
     3361 tcagcgccgc ttcggcggcc tcggtcgcgg cgacgagccg ctccgacttc aggcgcagga
     3421 gacgcttctt caggcccgag aggacgtcgg cctgctcctt ccaacgcgcc gcgcgcgagg
     3481 cctggcgctt gagcgaggtg caggcgcgct cgatctccga gacgatgtcg gtgaggcgga
     3541 ggaggttcgc gcgggtctcc tccagcttca cctcggcctg ctgcttccgg atcttgtact
     3601 tcgagatccc cgcggcctcc tcgatgaggc gccgccggtc ctgaggcttg gccgagagga
     3661 tcaggtcgat cttctgctgc tcgatgatcg cgtaggcgcg gacgccgagg ccggtcccga
     3721 ggaggaggtc ctggatgtcc ttcaggcgcg ccttgcggcc gttgaggatg tagtcgctcg
     3781 tcccgtcgcg ggtgacgcgg cgggtgagga cgacctcgcc accggtgtcg gcccactggc
     3841 cgccgcggga ctcgagcgtg agcgagacct cggccatgcc gagcgggcgg cgccgggcgg
     3901 agccgttgaa gatgacgtcc tccatcgtcg tcccgcgcag ggcgcgggcc gactgctcgc
     3961 ccaggaccca catcacggcg tcgcagatgt tcgacttgcc ggagccgttc ggcccgacga
     4021 tggcggtgag gcgggacgga agggtgaggg acgttcggtc gacgaacgac ttgaaaccgt
     4081 ggatctcgag ctttttcagt cggaacatgg cagcgtcagg cgggcgaccc taccacgacg
     4141 gagggggttc gtgccggaga gggccgtcgg gagcgtcccc gttcaggcga cacgcgccgg
     4201 ggggcggccg gggacggcgt ggagggcgcg gaggagctcg ccgcgggacg cgaccgagag
     4261 ctttcggaac gcgtgcttga ggtggtcctt cacggtgtac tcgctgatga agagctcgcc
     4321 ggcgatctcg gagttgcgca ggccgcgcag gacgcccgag acgacctcgg cctcccggtc
     4381 ggagacgccg cgggccgcca ggtggggccg gacgtcgtcg atcgtcggcg cggcccgctc
     4441 cttcaggagg acgatccggg ccggcggggc gacggaggtg ccgtccccgg ggcagtcgac
     4501 gatccgcgcc tcgaggcacc gcccgtcggt gagagcgatc ctctccgtgg ccggcgcctc
     4561 gcgcccggcg agctggagga ggtggggcac gagagggatc gcccgccggt ctcccgacag
     4621 gacggtcggt ccctcctccg cctcgcggga gaggagctcc tcggcggcgc cgttcgcgta
     4681 gaggatcgtc cctccccggt cgaggatcat cgcgggggct tccgtcgcct cggcgagggc
     4741 gaggaggcgc tcctgcgcgc agcgggcgac gggcgcctcc gcgaggcggg tcaggagctg
     4801 gcaggcgagc ggcgcgagga gggagaagcg catcgactcg gcgcccgtcc aggggcggaa
     4861 gcgccgcctc acgaggtaga ggcgtcccct ccagcccccc tcctcgccga acgggaccgt
     4921 gaggacgttt ccgttcggga gcccgccgtt gcgtgaggcg ggcgcgaggc ggaggcccga
     4981 atcccttcct ccggcgagcc tcctgcggaa cctccgcccg gaggaggggc agaggaggac
     5041 gagctcgaag gcgtccggcg cgagagcggc tccgagggtc gccacgaggg cctcgaggtc
     5101 ggcgtcgcgc ggggcgctga agggaacggc gtcgatcagc gagcggggca tcgtctcacc
     5161 ggaagggatt ctagagggtc gagctcgttc agaatttgtc gacgcttcga ggacaatcgc
     5221 cctgtcgccg tggtagcctt ccgtccgttc gcgaggcacc ccctcccgaa tcggaggccc
     5281 gaaaacatgc tcagcttcga ggtccgcaag ccgggtggcg agtcggagat ccgcaaggtc
     5341 caccgggacg tgatccatct cggcgcttcg tccgggaacg atctggtcgt aagggcacga
     5401 ggtgtcctcg gccggcacgc ccgcatctcc gtcgcgggcg aggagctccg tctcgacgtc
     5461 ctcgggaccg ctcccggctc ggacgtgtac ctgaacggtg cgatcgtcaa gtcggctgcg
     5521 ctcgcggcgg gcgaccggat ccagattggc gaggcgacga tcactctgct gaacggcccc
     5581 gccccgaatc cgaacaggat cgccgccccg ccgccgcgcg ggagggacgc cttctcggtc
     5641 gcttcggccg ccctttcccc gctgccgccc gctccggctc cgcccgcccc ctcggcgccg
     5701 cgggcgccgg agccgcggcc gaggtcgtac gagccggtgc acgccgtccg gccggccgag
     5761 aaccgctcgc tctcggcggc gcagggcttc cgcctcgccg acgtctgggg cgagatcttc
     5821 ggacggctcc gcgcctcggc caccctcgag gagaggctcc aggaggtcgc gtcgtacctc
     5881 tcctcctcca cgcccgtcca cagcgtcgcg ttcctctccg tcgccgacag ctcgacggga
     5941 gaggcgatcg ccgcgatctg gaaggggacg ctgccgcggc tcggccgccg ctccgtcgac
     6001 gaggtcttcg ccgccggcgg gccgactgac gtcttcgaag cgggcatgcg cctccgggtc
     6061 tacccggtga cgcggccggg cgccgaggcg gtgggcctcc tcgtcctccc gatggagctg
     6121 gccgccgacc cgctcctgaa cgacctcgcc gtcgcgctcg cggcggcgat cgggttcgtc
     6181 catgccgagc gcgccgacgc gccgttcgga gagcgcgcca gcgctcacgc ccccgcggcg
     6241 atggtgaccg cgccgctcga ggaccccgtc accgcggtcg tcggctcttc cctcgcgatc
     6301 cagaacctcc gcgtctctct ccagcggatc gcgtcggccc gcgcgcccgt cctcgtgatc
     6361 ggcgacgtcg gcaccggcaa gacgctcgtc gccgagacgc tccacgcgct ctcgccgcgg
     6421 cgtccgcgcc cgctcgtgcg gatctccgcg accgcctcca ccgcggcggc cctcgaggag
     6481 gacctcctcg ggaccgccgg cccgcccgac cggcggcgcc gtccgggccg tctcttcgag
     6541 gccgacggcg ggaccctcct catcgaggag gtcagcgagc tccccgcgcc gacccaggcg
     6601 ctcctctaca ggctcctcga ggcgcgcgag gtcgtcgcac ccggcggccg gtccgtcccc
     6661 gtcgacgtcc gcgtcatcgg cacctcttcg cggcccctca cgcgggcggt cgaggagggg
     6721 acgttccggg acgacctcta ctaccggctc tcggccctca cgctccgtct cccgacgctt
     6781 cgcgaccgca aggaggacat ccccgcgctc ctcgacgcgt tcgccgcgcg ccacggcgga
     6841 ccgcccgcgg cggccttcga cgtcgaggcg atgaacgccc tcctcgactt caactacgcg
     6901 gggaacgtcc gcgaggtcga gaacgaggtt cggcgcctcg cggcgcagct cggagcgggg
     6961 cccgtccgcc tctccgacct cgacgcgaag ttcggagacg agcacgtcga gctgacgctc
     7021 gccgagagcg acgacctcaa ggagatcgtc gagaaggtcg agcgccaggt catcgagcgc
     7081 gtcatgcgca aggtgcgcgg caaccagtcc ctcggcgccc gactcctcaa catctcccgc
     7141 ggctccctca tcgcgaagat gaaggagtac gacatcaagg acttccggta cctgaaacgc
     7201 caggattagg taggagctct tgatgagggg tgggggtccg gggggccgcg cacggaccca
     7261 gcagtccgct cgaaccgccg atgagtgggc gggggtccgg ggggcgcgcg aagctcagcc
     7321 ccccggcgag gcagcccccc ggcgagcgcg gcaggactga cagcggtcct ggatcgtggc
     7381 tgggggggcc ggggggccga acggttcagc ccttcccctt cgaacgccgc ctcttcccgg
     7441 aggcggcgtt tccctttccg gcctccgcgt cctcgccgat ccggagcgtc agcgcccaga
     7501 agacgaacgg caccgcgaac ttcccgacga tgtcgaagaa gtagcgcccc gtcgcgacga
     7561 cctccctctg ccagcgcgg
//
LOCUS       DAOTEZ010000192         7551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_6912_length_7551_cov_4.345251, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000192 DAOTEZ010000000
VERSION     DAOTEZ010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7551
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6912_length_7551_cov_4.345251"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..497)
                     /locus_tag="P5164_11090"
     CDS             complement(162..497)
                     /locus_tag="P5164_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HRY44478.1"
                     /translation="MAKTRTKRAPKVTTTPFDVAEHLRTPDEMAAYLDAWLDEAPDDS
                     VGIARALGDIARARGMSQVARDAGLSRESLYRALGAKGNPSFATVLKVAKALGVRFHA
                     QPTHHGGLP"
     gene            complement(503..796)
                     /locus_tag="P5164_11095"
     CDS             complement(503..796)
                     /locus_tag="P5164_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005407702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRY44479.1"
                     /translation="MKVEETEEFRRWLDRLADRDGRARILVRIDRLIHGNPGAHRNLT
                     EGISELRIDFGPGYRVYYSLQGSTLLLLLAGGDKASQRRDIAVARTLKRSFLG"
     gene            complement(847..2295)
                     /locus_tag="P5164_11100"
     CDS             complement(847..2295)
                     /locus_tag="P5164_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44480.1"
                     /translation="MKTERQRSRARSGGRIALLVLLALVFSRGAGAATVKPLSSGRLF
                     LEASDGKVEVEVVPGASAGVSVVDARTGTDLGKRLESKAREDGTGLRLVPGDPKSEER
                     PVVKLRVRPGQRLEVTGSRLDVSVTHLAGPPAEAAAPRVEPTIVGRLEKSTFRATGLI
                     GPDVGLFLTTDGGEIRLDGCETLVDARVTGGSLRVMDHRGLLRITGRDADLHLEGGTS
                     EPTVNLTGGTATFRDGSGPVALSANGAKVWVDQWTGALRIEAAHGNLDVARSATKLVD
                     LKLTDTFARLGQGSGRANVSCDGGAIDVDGFQGPMTIAATDRASASASGVTGALRLTG
                     TDGARLEVKDCRGAVTATSTGGTVAVERAASLKLDAVDSEVTVRAVPKLLPMTLRSCT
                     GELDLVETLGSPAVSVSLGSRLGVRLAEPCAVQASGPGAKLGESIACSGCDLKLPNHP
                     ALGPPRQYYRGTRRSVLALTLDEDSRVTIEAK"
     gene            complement(2330..5125)
                     /locus_tag="P5164_11105"
     CDS             complement(2330..5125)
                     /locus_tag="P5164_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HRY44481.1"
                     /translation="MRRRSPALPRRFALAALLLAIGLPAGAAEAAGGAEVSVANLRAE
                     GLENPLGLDEPRPVLSWQLRSAGRGVEQTAYEVRVRHEGRTVWATGRVASSASTGVPY
                     GGGPLLSSTRYEWQVRVWDGTGRRSRWSEPAWWETGLLAESEWRARWVEPGEEEGAAE
                     DRPSPMLRGEFRVEGVIRRARAYATSLGLYELEINGRRVGDRLFTPGWTSYGKRLAFQ
                     TYDVTELLRNGENVVGATLGDGWYRGRLHRAGGVRRNVYGERLALLCQVRVEFEDGRV
                     AVFGTDAGWRWATGAIRSADIYDGETYDGRLEQPAWSAPGFDASGWNPVAVVSPPRRA
                     LVATASPPVRKLAEVKPVKVWRSASGAHLVDFGQNLVGFVRVRVRGPAGQEVTLRHGE
                     ALDAAGELYTANLRSAAQRVTWILRGGDEEVREPRFTFQGFRYVSVEGWPGEPGPESL
                     TALVVGSDLPATGRFTSSSALLNRLQENIVRSQQGNFLDVPMDCPQRDERLGWTGDTQ
                     VFARTAAFNRDVSGFLGKWLADLALEQREDGAVPNIVPNVAGARDPHAWASAGWGDAA
                     VLVPWTLYEAYGDARVLERPYPSMRAWVEYVRRQAGPGLVWRGGDHFGDWLAFSSSAP
                     DDPGTATAKDLVATAYFARSAELLGRAAGLLGKAEDAREYADVATRVREAFVREFVTP
                     AGRVGENTQTAYALALAFDLLPEALRPEAARRLAEDVRRRGHLTTGFLGTPYLCQVLA
                     RFGHLDLAYRLLMREEHPSWLYPVTRGATTIWERWDGIRPDGSFQDPGMNSFNHFAYG
                     AIGEFLYRVVAGLDSDEAEPGYRHVVARPRPGGGLTSASARLETRYGEAASGWELAGN
                     RMTVRVTVPPNARGTIVLPGAAGRAVREGGVPVETAVGVRGTRIDAGDFVVEVGSGRY
                     ELSWETERSDTKPEVKP"
     gene            5347..>7551
                     /locus_tag="P5164_11110"
     CDS             5347..>7551
                     /locus_tag="P5164_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44482.1"
                     /translation="MPLQAGALLGLAALSSVVSATARAQAPVLPEPAYENSLQIGYFC
                     LNDCYSSTEKMQQQANLLRSRVGEGPYVKLGIFYYSNVGMSWPLPADPATAVLNPTVE
                     SNLRTMIDRAEAAGIGFHVSGIFGVTRAVSLYGPAVTEDRRNAQWLSTGAKSWITTSR
                     YARRLRAHLEAKVRLYAQLFARLQKEYPETLLTLAADGEIELSSDGYDDNKPYQQQVL
                     ADYSPFAVAEFRDWIRNTGLYGPGGPFDGQGRTASGTKYRTGDDAADLAAFNADFGVG
                     FGTWQLQYFDWDLADPVNADTNAIRAAEVADPSFSPLPTSGPRYIAGGFDAPRTWNAG
                     SPAFWQLWLQFREALVANYVRDVSEWLVSTTTPEGWSLPPDRVYTYQIPSDYYLGTYP
                     GCPNPERRYLTSASTFDSADVGPTASLGVTVFDGYYWDGSQNYYKATSEFLFPEIARR
                     RPWNWGLIESNLSWPISAVETDPAAVAARIDRAWAAGMHLYGYYPWPLDPNEEQTVLL
                     DGLGLFARNVWTEPYDAALRHYAPAVVRGLASELTGSDVRFTWAPAIFASHPGFQWLS
                     WKDFLRFEIVRAGAPDAPAGSWTLVKSVSNGYVTTLPVSSLTGAATWYRIRAVSKNGL
                     TGEMSPPIEVPCPRAAVEALSITPSVVKAGKGATIAWTVSGATSATLAGQTAALPSGT
                     KTVVPSTTTSYLFRATNACGATEKTVTLNVLPATGGVNLAAPVITAPGVGQVL"
BASE COUNT         1079 a         2865 c         2489 g         1118 t
ORIGIN      
        1 gcccgtggtc caacgagccg ggcggccccg gcgtcaccaa cttcaccgtc gtgccgtagg
       61 agcgcccgag agcgacgggc tacttcgcct cgatcgtcac gcgcgagtcc tcgtccggcg
      121 tcagcggcga gatggaagcg gagcgcgttc gccccgttgg ctcagggtaa gccgccgtgg
      181 tgagttggct gtgcgtggaa gcgcaccccg agggccttgg cgaccttcag caccgtggcg
      241 aagctcgggt tccctttcgc cccaagggcc cgatagaggc tctctcggct gaggccggca
      301 tctctggcga cctgtgacat gcctctcgcc cgggcgatgt cgccaagggc gcgtgcgatg
      361 ccgaccgagt catcgggggc ttcgtccagc catgcgtcga ggtacgccgc catctcgtct
      421 ggcgtacgga gatgctccgc cacgtcgaac ggagtggtgg tgaccttcgg agctcgcttg
      481 gtgcgtgtct tagccatgat gtttacccca ggaaggagcg cttgagagtt cgggcgacgg
      541 caatatctcg ccgctgagaa gccttgtcac cgcctgcgag caggagaagc aacgtgctgc
      601 cctgcagcga gtagtagaca cggtagccgg ggccgaaatc gatccggagc tcggaaatgc
      661 cctcggtgag gttcctgtgg gcgcctggat tcccgtggat gagccgatcg atacgaacga
      721 gaatcctcgc gcgcccatct ctgtcagcga gccggtcgag ccaacgccgg aactcttcgg
      781 tctcctcgac cttcacgatc cggagtgtag cgcatgggct acacaatggc ggccggcagc
      841 ctcgggctac ttcgcctcga tcgtcacgcg cgagtcctcg tccagcgtca gcgcgaggac
      901 cgagcgccgc gtcccgcggt agtactgcct cggcgggccg agcgccgggt gattcgggag
      961 cttcaggtcg catcccgagc aggcgatgct ctctccgagc ttcgctcccg gtccgctcgc
     1021 ctgcacggcg cacggctcgg cgagccggac gccgagccgc gagccgaggc tcaccgagac
     1081 cgccgggctc cccagcgtct cgacgaggtc gagctctccg gtgcagctcc gcagcgtcat
     1141 cggcagcagc ttcggcacgg cgcgaacggt gacctccgaa tcgacggcat cgagcttcag
     1201 gctcgccgcc cgttccaccg ccacggtgcc ccccgtcgac gtcgccgtca cggctccacg
     1261 gcagtccttc acctcgagcc gcgcgccatc cgtccccgtg agccggagcg cgcccgtcac
     1321 gccgctcgcg ctcgcgctcg cgcggtccgt ggccgcgatc gtcatcggcc cctggaaccc
     1381 gtcgacgtcg atcgcgccgc cgtcgcagct cacgttggcc cgccccgagc cctggccgag
     1441 tcgggcgaag gtgtcggtca gcttcagatc gacgagcttc gtcgccgaac gggccacgtc
     1501 gaggtttccg tgcgccgcct cgatccggag cgctcccgtc cactgatcca cccagacctt
     1561 cgctccgttc gccgagagcg ccaccggccc gctgccgtcg cggaaggtgg ccgttcctcc
     1621 ggtcaggttc acggtcggct ccgacgtgcc cccttcgaga tggaggtcgg cgtccctgcc
     1681 tgtgatcctc aggaggccgc ggtgatccat caccctcagc gacccgccgg tcacccgagc
     1741 gtcgacgagg gtctcgcacc cgtccagccg aatctcgccc ccgtcggtgg tcaggaacag
     1801 cccgacgtcc ggtccgatca gcccggtcgc ccggaaggtc gacttctcca gccggccgac
     1861 gatcgtcggc tcgaccctcg gggcggcggc ctcagcgggc ggaccggcga ggtgggtgac
     1921 cgacacgtcg aggcggctcc cggtcacctc cagtcgctgg cccggcctca cgcggagctt
     1981 cacgaccggc cgctcctcgc tcttgggatc gcccggcacg agccgcagcc cggtgccatc
     2041 ctcgcgtgcc ttcgactcca gccgcttgcc gaggtcggtc cccgtccgcg cgtcgacgac
     2101 gctcaccccg gccgaggccc cgggcacgac ctccacctcc acctttccgt cgctggcctc
     2161 gaggaagagg cggcccgacg agagaggctt caccgtggcc gcgcccgccc ctcgggagaa
     2221 gacgagagcg agaagaacga ggagggcgat cctgccgccg ctccgggcac gcgaccgctg
     2281 tcgttccgtc ttcatgagcg ctctccctat cctgaagtgt agcgacccct cacggcttca
     2341 cttcgggttt cgtgtcgctt ctctcggtct cccaggagag ctcgtagcgc cccgagccca
     2401 cctcgacgac gaagtctccg gcgtcgatcc tcgtgccgcg cacgccgacg gccgtctcca
     2461 ccgggacccc gccttcgcgg acagccctcc ccgccgcgcc ggggaggacg atcgtcccgc
     2521 gggcgttcgg gggcacggtc acgcgcaccg tcatccgatt tccggcgagc tcccacccgg
     2581 atgcggcctc gccgtacctc gtctcgagcc tcgccgaggc cgacgtgagg ccgccgccgg
     2641 gccggggccg ggcgacgacg tgccggtacc cgggctcggc ctcgtccgag tcgagccccg
     2701 ccaccacgcg gtagaggaac tcgccgatcg ccccgtaggc gaaatggttg aacgagttca
     2761 tccccgggtc ctggaacgag ccgtcggggc ggatgccgtc ccaccgctcc cagatggtcg
     2821 tcgcgccgcg ggtcaccggg tagagccacg aagggtgctc ctcgcgcatc aggagccggt
     2881 acgcgagatc gaggtgcccg aaccgcgcga ggacctggca gaggtacggc gtcccgagga
     2941 accccgtcgt caggtgcccg cgccgccgca cgtcctcggc gagccgccgc gccgcctcgg
     3001 ggcgaagcgc ctcgggcagg aggtcgaaag cgagcgcgag cgcgtacgcc gtctgcgtgt
     3061 tctcgcccac gcgcccggcc ggcgtcacga actcgcggac gaacgcctcc ctcacgcggg
     3121 tggcgacgtc ggcgtactca cgggcgtcct ctgccttccc gagcagcccg gccgcccggc
     3181 ccagcagctc ggccgagcgg gcgaagtagg ccgtcgccac caggtccttc gcggtcgccg
     3241 tgccggggtc gtccggggcg ctcgaggaga aggcgagcca gtcgccgaag tggtccccgc
     3301 cccgccagac gagcccggga cccgcctgcc gccgcacgta ctccacccag gctctcatgc
     3361 tcgggtacgg acgctccagg acgcgcgcgt cgccgtaggc ctcgtagaga gtccacggca
     3421 cgaggacggc cgcgtcgccc cacccggccg aggcccaggc gtgcgggtcc ctcgccccgg
     3481 ccacgttcgg gacgatgttc gggacggctc cgtcctcccg ctgctcgagg gcgaggtcgg
     3541 cgagccactt cccgaggaag cccgagacgt ccctgttgaa tgcggccgtc ctcgcgaaga
     3601 cctgcgtgtc cccggtccac cccagccgct cgtcccgctg cgggcagtcc atcggcacgt
     3661 cgaggaagtt cccctgctgg ctccggacga tgttctcctg cagccgattc agaagcgcgc
     3721 tcgagctcgt gaagcggccg gtggcgggaa ggtccgaccc gacgacgagg gcggtcaggc
     3781 tctccgggcc tggctctccc ggccagccct ccacggagac gtagcggaag ccctggaacg
     3841 tgaagcgcgg ctcccgcacc tcctcgtcgc ccccgcggag gatccacgtc acccgctgtg
     3901 cagcgctccg gaggttcgcg gtgtagagct ccccggcggc gtcgagagcc tccccgtgcc
     3961 ggagcgtcac ctcctgcccg gcggggcccc gaacgcggac ccggacgaac ccgacgaggt
     4021 tctgcccgaa gtcgacgaga tgcgcgcccg aggccgatcg ccagaccttc accggcttca
     4081 cctcggcgag cttcctcacc ggcggtgagg ccgttgccac gagcgctcgc cgcggaggtg
     4141 agacgacggc cacgggattc catccggagg cgtcgaaccc gggcgcgctc caggctggct
     4201 gctcgagccg gccgtcgtac gtctccccgt cgtagatgtc ggcgctccgg atcgcgccgg
     4261 tcgcccacct ccagccggcg tccgttccga agacggcgac ccgcccgtcc tcgaactcca
     4321 ccctcacctg gcagagcagg gccagccgct ccccgtaaac gtttcgccgc accccgccgg
     4381 cgcggtggag ccggccgcga taccagccgt ctccgagcgt cgccccgacg acgttctctc
     4441 cgttccgaag gagctccgtc acgtcgtacg tctggaaggc gaggcgcttg ccgtagctcg
     4501 tccagccggg cgtgaagagc cggtctccca cccgtctgcc gttgatctcg agctcgtaga
     4561 ggccgaggct cgtcgcgtag gcgcgggccc gccgtatgac gccctccacc cggaactcgc
     4621 cccggagcat cggcgagggg cggtcctcgg cggccccctc ctcctcgccg ggctcgaccc
     4681 accgcgcccg ccactccgat tccgcgagga gccccgtctc ccaccacgcg ggctcgctcc
     4741 agcgcgagcg gcgacccgtg ccgtcccaga cgcggacctg ccactcgtac cgggtcgagg
     4801 agaggagcgg gccgccgccg tacggcacgc cggtcgacgc gctcgacgcg acgcgcccgg
     4861 tcgcccacac cgtccggccc tcgtgacgca cgcgcacttc gtacgccgtc tgctccacgc
     4921 cgcgccccgc ggagcgaagc tgccacgaca ggaccggccg aggctcgtcg aggccgaggg
     4981 gattctccag tccttcggcc cggaggttcg cgacggagac ctcggcgccg cccgcggcct
     5041 cggcggcgcc ggcggggagg ccgatggcca ggagtagggc cgcgagggcg aagcggcggg
     5101 gaagggcggg ggagcggcgt ctcacggggg gcactcggat cgggcgtcgt accagagagc
     5161 ccggcgggtg tccactcggc attctgtccc ggagccttcg ggagatatca ttcccggaga
     5221 cggactcgca tctgcgtgcg agctgtgttc agaggtcggg tgtacctcgc tggaggcatg
     5281 gccatgagga cggaaaccgg tggcgaaatg cgtcggaccg acgtgaagct ggcgcgaaga
     5341 ctccgggtgc cactgcaggc cggagctctc ctcggtctcg ccgcactttc ctccgtcgtc
     5401 tccgcaacgg cgcgggcgca ggcgcccgtc cttcccgaac ccgcgtacga gaactcgctt
     5461 cagatcgggt acttctgcct gaacgactgc tattcgtcga ccgagaagat gcagcagcag
     5521 gcgaatctgc ttcggtctcg tgtcggcgag ggaccctacg tgaagctggg catcttctac
     5581 tactccaacg tcggaatgtc ctggccgctt ccggccgacc cggcgacggc cgtcctcaac
     5641 ccgaccgtgg agtcaaacct gcggacaatg atcgatcgag cggaggcggc aggcatcggg
     5701 ttccacgtct cgggcatctt cggggtcacc agagcggtct cgctctacgg cccggccgtc
     5761 accgaggacc gcaggaacgc gcagtggctc tccacggggg caaagtcctg gatcacgacc
     5821 tcgcgatatg cccgtcgact ccgggcacac ctcgaagcga aggtccgcct ctacgcgcag
     5881 ctttttgcgc gtctccagaa ggagtacccg gagacgctcc tcaccctcgc ggccgacggg
     5941 gagatcgagc tcagctcgga cggctacgac gacaacaagc cctaccagca acaggtcctc
     6001 gccgactact ccccctttgc cgtcgccgag ttccgcgact ggatccggaa cacgggactc
     6061 tacggccccg gcggcccctt cgacgggcag gggcgcaccg cgagtgggac gaagtaccgg
     6121 acgggcgacg acgcggccga cctcgcggcc ttcaacgccg acttcggcgt cggcttcggc
     6181 acctggcagc ttcagtactt cgactgggac ctcgccgacc cggtgaacgc cgacacgaac
     6241 gccattcgcg ccgcggaagt cgccgacccg agcttctccc cccttccgac ttccggtccg
     6301 cgctacatcg ccggtggatt cgacgcaccg cgcacctgga atgccggcag tccagccttc
     6361 tggcagctct ggcttcagtt ccgcgaggcg ctcgtcgcga actacgtccg cgacgtctcc
     6421 gagtggctcg tctcgacgac gaccccggag gggtggagcc tcccccccga ccgcgtctac
     6481 acgtaccaga ttccgtccga ctactacctc ggcacctacc ccggctgccc gaatcccgaa
     6541 cggcgctacc tgacctcggc cagcaccttc gactcggccg acgtcggccc cacggccagc
     6601 ctcggcgtca cggtcttcga cggctactac tgggacggct cgcagaacta ctacaaggcg
     6661 acgagcgagt tcctcttccc ggagatcgcc cgtcggcgcc cgtggaactg gggcctcatc
     6721 gagtcgaacc tgagctggcc gatctccgcc gtcgaaaccg acccggccgc cgtcgccgcc
     6781 cgcatcgacc gtgcctgggc cgccggaatg cacctctacg gctactaccc ctggcccctc
     6841 gacccgaacg aggagcagac cgtcctcctg gacggcctcg ggctcttcgc ccggaacgtc
     6901 tggaccgagc cgtacgacgc cgcgctccgg cactatgccc cggcggtcgt ccgcggcctg
     6961 gcttcggagc tgaccggcag cgacgtccgc ttcacgtggg cgcccgccat cttcgcgagc
     7021 catccggggt tccagtggct ctcctggaag gacttcctcc gcttcgagat cgtccgagcg
     7081 ggggcgccgg atgccccggc gggatcgtgg acgctcgtga agtcggtctc gaacggctac
     7141 gtcacgaccc tcccggtctc ctccctcacc ggggccgcca cctggtaccg gatccgggcg
     7201 gtcagcaaga acggcctgac gggcgagatg agcccgccga tcgaggtccc ctgcccgcgg
     7261 gcggccgtcg aggcgctctc catcacgccg tccgtcgtca aggccggcaa gggcgccacg
     7321 atcgcctgga cagtctccgg ggcgacgagc gcgacgctcg ccggccagac cgccgcgctc
     7381 ccctccggga cgaagacggt cgttccgtcc accacgacct cctacctctt ccgtgccacg
     7441 aacgcgtgcg gcgccacgga gaagaccgtc acgctcaacg tccttcccgc gaccggcggc
     7501 gtgaacctcg ccgcgcccgt catcaccgcg ccgggcgtcg ggcaggtcct c
//
LOCUS       DAOTEZ010000193         7471 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_7008_length_7471_cov_6.005798, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000193 DAOTEZ010000000
VERSION     DAOTEZ010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7471
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7008_length_7471_cov_6.005798"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="P5164_11115"
     CDS             <1..649
                     /locus_tag="P5164_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44483.1"
                     /translation="LVRLIGQMLEKRREARPQSYREITERLVAILRSAGAGAGAGAGV
                     AAGLAESGDAASPGGTAAPRAATSPTQLVSEKPRSPSVPVRRVAGVLAAGAALLGLLL
                     WGALRSGPGEAPPARSVGPAPPPPSTAQLRITSVPWGVVVSVVDVATGKAVALPSPAD
                     TPLPLAVPPGRYRVTVVEPAPGTGRAETDTVAAAGRPALVALTLRPLEETVETLR"
     gene            646..2079
                     /locus_tag="P5164_11120"
     CDS             646..2079
                     /locus_tag="P5164_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44484.1"
                     /translation="MKRLLPVLAVVLLARVPASAQVESALLSKVTFNLTNPGGKSLAM
                     GGAFTAIADDATAGIANPAGLGLLSSFEAGVSGKRVDDVIGLVTARSTATGGLSAPYP
                     SPRAVNSDISARSSLGEFAGIVIPISRRVVAAVSFAENLSFEGDPGPDGYAYIELRDN
                     RSGGTTRRDFLYEFREHGAVSLRNRLVALSAAYRVTERLRLGAGLTLNRMELDLLGDE
                     GGPHRIVNRTFTTPTQVRTLTTNMAIRDLDGNVLGFLVGAHVDLLADGKLTAGAVYRQ
                     TSKAEGTFEIGGDVPTPLAGQKTRPFSARVPRDAAIGLAAQPFPGLTVAAEAQWIAYG
                     DFIDRSLPTVSYAGFVGPAPGMFLEDLLAEMRPPDDVVVPRLGVEYVAASGDLLLAFR
                     LGYHREPARGVTANLVATEETTGAAFDVDDPPFSGGVRTVYDGGRPDDRFSGGLGATI
                     SRKVSFDLAFDLGRSSRVLSASLFYRF"
     gene            complement(2123..3061)
                     /locus_tag="P5164_11125"
     CDS             complement(2123..3061)
                     /locus_tag="P5164_11125"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44485.1"
                     /translation="MPRGLGEILAESGDLTFRRLRRASEWRRVHGGRIDRALLETGAV
                     PEETLLDALCRLTGLPSVSRERLAAASREAVEALPVDTRRRLRALPFDRRGEVLHVAV
                     VDPGNPVLETGLVASTGCDIRLYVTAEPILEDLLARWEAAPPAERHGPRRAAGERPPR
                     EEPDAGPAATPAAARAAETDPFGRLARALLVDALDEGAEAVEIGAEGRGAVVRSYAGG
                     AVLSSRSIPSPVLGPLFAWLVARSRPAAPLDEGGILLERAHRRFRVQVRSTADGTAWL
                     LLSPEAAAPAAPAENEVCLHETADGDVFCPACGAPV"
     gene            complement(3137..3745)
                     /locus_tag="P5164_11130"
     CDS             complement(3137..3745)
                     /locus_tag="P5164_11130"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRY44486.1"
                     /translation="MSRPLTILMDEHRIIEQALGSLETCAIEVDGGLAIERAVVRDYA
                     DFLKGFADAWHHGKEEDILFRRMVERGFSAESGPVAVMLHEHRQGRELVGRIAAVGAG
                     EGPVAPEERASFLAASRGFVPLLRQHIQKEDNILYPMSARVLTPEEFEKMLADFEAFE
                     RAAGAVGTRDRLSAVAEKLVAAFRPDEARMAAGAAGGCWSRG"
     gene            complement(3818..4225)
                     /locus_tag="P5164_11135"
     CDS             complement(3818..4225)
                     /locus_tag="P5164_11135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRY44487.1"
                     /translation="MFSRTSLYALRTLGYLAAHRERWVLGREIAEETGIPPNYLSKIL
                     AQLRKRGFVVSQKGWGGGFRMKGTGAGTTISAVLETFDGRREKDACAFGLGRCDAADP
                     CPLHPHWEKVREEYERMLRTVTLRDLGGSGSAA"
     gene            complement(4308..6080)
                     /locus_tag="P5164_11140"
     CDS             complement(4308..6080)
                     /locus_tag="P5164_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HRY44488.1"
                     /translation="MDDLRQFLAALRAEGDLLEIEAEADPRLEIPEIHRRVIAQGGPA
                     LLFRRPKGSSFPVVTNLFGTAKRIDLAFGKRPLDFVKTAVRASHELLPPTAGKLWGFR
                     SFFREALRVGTREVSAARVTEVVETEPDLTRLPALTQWPEDGGPFLTLPLVYTEHPEK
                     REHNLGMYRIQIHEARQTGVHWQIGKGGGFHHHVAEAKGEALPVTVFLGGPPALIIAA
                     IAPLPEGLPELLLGSLLLGKKLPLARVPGHPHRLVAGAELAITGKVPPRVRRLEGPFG
                     DHYGYYSLAHDYPVLDVERVYRRRDAIFPATVVGKPVQEDFLIGDFLQELLSPLFPVV
                     MPSVVSLKTYGDTGFHSLAAAVVKDRYGREAISTAFRILGEGQLALTKFLLLTDARVD
                     LDDFRTLLETVLSRCRFETDLFVVSNVAMDTLDYTGPKVSEGSKGVLAGLGAPVRDLP
                     RAFEGALPAGARAAAVFCGGALCVSGPSWEEDRAFASRLAADPAVAAWPLVALVDDAE
                     AAAKDARSFLWHVFTRFEPGADVHAARTEVVRNQVARTAPIVLDARMKSWMPAVVEAD
                     PATIALVDRRWREYFPARGTGARL"
     gene            complement(6118..6819)
                     /locus_tag="P5164_11145"
     CDS             complement(6118..6819)
                     /locus_tag="P5164_11145"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44489.1"
                     /translation="MRERRGVPPRATTSISVPLQLFPRLPHAPPPFPLLLGLHGYGMH
                     AATLFPLLARMAPEGWGVVAVEGPQSAFLPGEALGPSGARGFHWGVSPRHEDNQAVHR
                     AAVAEAIAWSVARGADPARVSLLGFSQPCSFDYRLALAPPHGRPFHAVVALCGGLPGE
                     WTEPGPGTDATSATHALHVSTDADEYYPLERVAPFEALLATRFAGARHEIHHGGHRIP
                     STAIPVVRAFLEAHG"
     gene            complement(6816..7211)
                     /locus_tag="P5164_11150"
     CDS             complement(6816..7211)
                     /locus_tag="P5164_11150"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRY44490.1"
                     /translation="MLLSRAAETALKALPHLDPRGPEAPGREARDLALACGEPAPWLG
                     KVLQKLARAGVLRSKRGRTGGFVLGRPAAEITLADVLLAIEGADDLERLLDVPAGPAG
                     PPLLPARDALLSALRGTTLESLVPPGDSR"
     gene            complement(7278..>7471)
                     /locus_tag="P5164_11155"
     CDS             complement(7278..>7471)
                     /locus_tag="P5164_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HRY44491.1"
                     /translation="PRPDPADPKGTVVDVKAGKPLKRPVTLAEMKAAKLFAGSPLLTI
                     GRLSVVPLTTEQYAFVVGE"
BASE COUNT          985 a         2697 c         2809 g          980 t
ORIGIN      
        1 gctcgtgagg ctgatcggtc agatgctcga gaagcgccgc gaggcgcggc cccagagcta
       61 ccgcgagatc accgaacgcc tcgtcgcgat cctccgttcc gcgggcgcgg gcgcgggcgc
      121 gggcgcgggc gtggccgcgg gcctcgcgga gagcggcgac gccgcgtctc ccggcggcac
      181 ggccgccccg cgcgcggcga cttccccgac gcagctcgtg tcggagaagc cgcggagccc
      241 gtccgtcccg gtgcggcgcg tcgccggcgt cctggcggcg ggggccgcgc tcctcgggct
      301 gctcctgtgg ggcgcgctcc ggagcggccc cggggaggcg cccccggccc gttccgtcgg
      361 ccccgctccg cctcctcctt cgaccgccca gctgcggatc acgtcggtcc cgtggggcgt
      421 cgtcgtctcc gtcgtcgacg tggccactgg aaaagcggtt gcgctgccgt cccctgccga
      481 caccccgctc cccctcgcgg ttcctcccgg gcggtaccgc gtcacggtcg tcgagccggc
      541 ccccgggacc ggccgcgccg agaccgacac ggtggccgcc gccggaaggc ccgccctcgt
      601 cgcgctcacc ctcagaccgc tcgaagagac cgtggagacc ctgcgatgaa gagactcctt
      661 cccgtcctcg ccgtcgttct cctggcccgg gtcccggctt cggcccaggt ggagtcggcg
      721 ctcctgtcga aggtgacgtt caacctgacg aacccgggcg gaaagtcgct cgcgatgggg
      781 ggcgccttca ccgccatcgc cgacgacgcg accgccggca tcgcgaaccc cgccggcctc
      841 ggcctcctct cgtcgttcga ggcgggcgtc tcggggaagc gggtcgacga cgtcatcggc
      901 ctcgtcaccg cgcggtcgac ggcgacaggc gggctctccg cgccctatcc gtcgcctcga
      961 gcggtcaact ccgacatctc ggcccgaagc tcgctcggcg agttcgcggg gatcgtcatc
     1021 cccatctcgc gccgcgtcgt cgccgccgtc agcttcgccg agaacctgag cttcgaaggg
     1081 gaccccggtc cggacggcta cgcctacatc gagctgcgcg acaaccggtc gggagggacg
     1141 acccggcgcg acttcctcta cgagttccgc gagcacgggg ccgtctcgct ccgaaaccgc
     1201 ctcgtcgcgc tctccgccgc ctaccgggtg acggagcgcc tccggctggg cgcgggcctg
     1261 acgctgaacc ggatggagct cgacctcctc ggcgacgagg gcgggccgca ccggatcgtc
     1321 aaccggacgt ttacgacgcc gacccaggtc cggaccctca cgacgaacat ggcgatccgc
     1381 gacctcgacg ggaacgtcct cggcttcctc gtgggcgcgc acgtcgacct cctcgcggac
     1441 ggcaagctga cggccggggc cgtctaccgg cagacgtcga aggcggaggg gacgttcgag
     1501 atcggagggg acgtcccgac gccgctcgcc gggcagaaga cgcgcccgtt ctcggcccgc
     1561 gtcccgcgcg acgcggcgat cggactcgcc gcgcagccgt tccccggcct gaccgtcgcg
     1621 gccgaggcgc agtggatcgc ctacggcgac ttcatcgacc gttcgctccc gaccgtgtcg
     1681 tatgccggat tcgtggggcc ggccccgggc atgtttctgg aggacctcct cgccgagatg
     1741 agaccgccgg acgacgtcgt cgttccgcgc ctcggggtcg agtacgtcgc ggcgtcgggg
     1801 gacctcctcc tcgcgttccg tctcgggtat caccgcgagc cggcgcgcgg cgtgaccgcg
     1861 aacctcgtcg cgaccgagga gacgaccggc gccgccttcg acgtcgacga tccccccttc
     1921 tccggcggcg tgcggacggt ctacgacggc gggcgccccg acgaccggtt ctcgggcggc
     1981 ctcggtgcca cgatctcgag gaaggtctcg ttcgacctcg cgttcgacct cggccggtcg
     2041 tcgcgcgtcc tgtccgcctc gctcttctac aggttctgac cgtccggcgc gggagcgcgc
     2101 cggagccggg ccgccccggc cttcagaccg gtgcgccgca ggcggggcag aagacgtccc
     2161 cgtccgccgt ctcgtgcagg cagacttcgt tctcggccgg tgcggccgga gccgccgcct
     2221 ccgggctcag caggagccag gcggtcccgt cggccgtcga gcggacctgg acccggaacc
     2281 gccgatgcgc tcgctccagg aggattcccc cctcgtcgag cggcgccgcc ggccgactcc
     2341 gtgcgacgag ccacgcgaac aacgggccga ggaccgggga gggaatcgac cgggaggaga
     2401 ggaccgcgcc gccggcgtag ctccggacga cggcgccccg gccctccgcg ccgatctcca
     2461 ccgcctcggc cccctcgtcg agggcgtcga cgaggagagc tcgcgcgaga cgcccgaacg
     2521 ggtcggtctc ggcggcgcgc gcggccgccg gcgttgccgc cggcccggcg tccggttcct
     2581 cgcgaggggg acgctctcct gccgctcgcc gcggtccgtg ccgctcggcc ggaggagccg
     2641 cctcccacct cgccaggagg tcctcgagga tcggctctgc ggtgacgtag aggcggatgt
     2701 cgcagcccgt cgaggcgacg aggccggtct ccaggaccgg gttcccgggg tcgacgacgg
     2761 ccacgtggag gacctcgccg cgacggtcga acgggagcgc gcgaagacga cgacgcgtgt
     2821 cgacggggag ggcctccacg gcctcgcggc tcgccgcggc gagcctctcc cgcgagacgc
     2881 tcggaaggcc cgtcagccgg cagagggcgt cgaggagcgt ctcctcgggg accgcccccg
     2941 tctcgaggag cgcccggtcg atcctcccgc cgtggacgcg cctccactcc gacgcgcgcc
     3001 ggaggcggcg gaaggtgagg tctccggact cggcgaggat ctctcccagc ccccgcggca
     3061 cgaacgctcc tccgttggga gaagagtaca cgggagaggg gccggcgcgg ccggccccct
     3121 cttccgtcgt ccggcttcag ccgcgggacc agcagcctcc cgcggcgccg gccgccatcc
     3181 gggcctcgtc ggggcggaaa gccgccacga gcttctccgc gacggcggag agccggtcgc
     3241 gggtgccgac cgccccggcg gcccgctcga acgcctcgaa atcggcgagc atcttctcga
     3301 actcctccgg ggtgaggacc cgcgccgaca tcgggtagag gatgttgtcc tccttctgga
     3361 tgtgctggcg cagcagcggc acgaagcctc gcgaggccgc gaggaacgac gcccgctcct
     3421 ccggcgcgac cggcccctcg ccggcgccca ccgcggcgat ccgtccgacg agctcgcgcc
     3481 cctgccggtg ctcgtgcagc atgaccgcca ccggccccga ttcggccgag aagccgcgct
     3541 ccaccatccg ccggaagagg atgtcctcct ccttgccgtg gtgccaggcg tcggcgaagc
     3601 ccttcaggaa gtcggcgtag tcgcggacga cggcgcgctc gatcgccagg ccgccgtcga
     3661 cctcgatcgc gcaggtctcg agggagccga gggcctgctc gatgatccgg tgctcgtcca
     3721 tcaggatcgt caggggtcgg gacatcacgt ctcctctgtc ggtcgtgtcg gtctcaatgg
     3781 atctggagat ccgttgatat ggcaaaaaaa gagaggctca cgccgcgctc cccgatccgc
     3841 cgaggtcgcg gagcgtcacg gtccggagca tccgttcgta ctcctcccgc actttctccc
     3901 agtgggggtg gagagggcag gggtcggcgg cgtcgcagcg gccgaggccg aacgcgcagg
     3961 cgtccttctc gcgcctcccg tcgaacgtct cgaggacggc cgagatcgtc gtgcccgccc
     4021 cggtcccctt catccggaag ccgccgcccc agcccttctg gctgacgacg aaaccgcgct
     4081 tgcggagctg ggcgaggatc ttcgataggt agttcggcgg gatccccgtc tcctcggcga
     4141 tctcgcggcc gagcacccag cgttcgcgat gagccgcgag gtagccgagc gtccggaggg
     4201 cgtagaggga ggtccgggag aacatcgcgt cgtctctcag tggatgaggt tatccgttga
     4261 gacggtcccg cgcaagtggc ggcgacccgt cccgcggtcg cgcggcctca gagccgcgcg
     4321 ccggtccctc gtgcggggaa gtactcccgc cagcggcggt cgacgagggc gatcgtcgcc
     4381 gggtcggcct cgacgaccgc gggcatccag ctcttcatcc gtgcgtcgag gacgatcggc
     4441 gccgtccggg cgacctggtt cctcacgacc tcggtcctcg ccgcatggac gtcggcccct
     4501 ggctcgaagc gtgtgaagac gtgccagagg aacgagcggg cgtccttcgc cgcggcctcc
     4561 gcgtcgtcca cgagggcgac gagcggccag gcggcgacgg ccgggtcggc cgcaaggcgc
     4621 gaggcgaagg cgcggtcctc ctcccacgac ggccccgaga cgcagagcgc gccgccgcag
     4681 aagacggccg cggcgcgggc gccggcgggg agcgcccctt cgaacgcgcg cggcaggtcg
     4741 cggaccggtg caccgaggcc cgcgaggacc cccttcgagc cctcgctcac cttcgggccg
     4801 gtgtagtcga gcgtgtccat cgcgacgttc gagacgacga agaggtcggt ctcgaagcgg
     4861 cagcgcgaga ggacggtctc gaggagcgtg cggaagtcgt cgaggtcgac gcgggcgtcg
     4921 gtgaggagga ggaacttcgt cagcgcgagc tgcccttcgc cgaggatccg gaacgccgtc
     4981 gagatcgcct cccggccgta gcggtccttc acgacggccg cggcgagcga gtggaagccg
     5041 gtgtcgccgt aggtcttcag ggagacgacg ctcggcatga cgacggggaa gaggggcgag
     5101 aggagctcct ggaggaagtc gccgatgagg aagtcctcct gcacgggctt cccgacgacg
     5161 gtcgcgggga agatcgcgtc gcgccgccgg tagacccgct ccacgtcgag gaccgggtag
     5221 tcgtgcgcga gcgagtagta gccgtagtgg tcgccgaacg gcccctcgag gcggcgcacc
     5281 ctcggcggga ccttcccggt gatggcgagc tcggcgccgg cgacgaggcg gtgcgggtgc
     5341 cccggcacgc gcgcgagcgg gagcttcttc ccgaggagga gggagccgag gaggagctcg
     5401 gggagcccct ccgggagcgg cgcgatcgcc gcgatgatga gcgcgggcgg cccgccgagg
     5461 aagacggtca cggggagcgc ctcgcccttc gcctcggcga cgtggtggtg gaagccgccc
     5521 cccttgccga tctgccagtg gaccccggtc tgccgcgcct cgtggatctg gatgcggtac
     5581 atcccgaggt tgtgctcgcg cttctccggg tgctcggtgt agacgagcgg gagcgtcagg
     5641 aacgggccgc cgtcctcggg ccactgcgtc agcgccggca ggcgcgtcag gtcgggctcc
     5701 gtctcgacga cctcggtcac gcgagctgcg gagacctccc tcgtccccac cctgagcgcc
     5761 tcgcggaaga acgagcggaa gccccagagc ttcccggccg tcggcgggag gagctcgtgc
     5821 gaggcgcgga cggcggtctt cacgaagtcg agcggcctct tcccgaacgc gaggtcgatc
     5881 cgcttcgccg ttccgaagag gttcgtgacg acgggaaagg aggagccctt cggacgccgg
     5941 aacagcagcg ccggccctcc ctgagcgatg acgcgacggt ggatctcggg gatctcgagc
     6001 ctcgggtcgg cctcggcctc gatctcgagg aggtcccctt cggctctcaa ggcggcgagg
     6061 aactggcgga ggtcgtccat cgtctgagga ggttacgacg gggcggggcc ggcgggttca
     6121 gccgtgcgct tcgagaaagg ccctcaccac cgggatcgcc gtcgagggga tccggtgccc
     6181 gccgtggtga atctcgtggc gggcgcccgc gaaacgggtg gcgaggaggg cctcgaacgg
     6241 agcgacgcgc tcgagggggt agtactcgtc ggcgtccgtc gagacgtgga gcgcgtgcgt
     6301 cgcggaggtt gcgtcggtgc cggggccggg ctccgtccac tcgcccggca ggcccccgca
     6361 gagcgcgacg acggcgtgga acgggcgccc gtgtggcggg gcgagcgcga gccggtagtc
     6421 gaacgagcag ggctggctga agccgaggag ggagacccgc gcggggtccg cgccgcgggc
     6481 gacgctccag gcgatcgcct cggcgacggc ggcgcggtgg acggcctggt tgtcctcgtg
     6541 gcgcgggctg acgccccagt ggaagccgcg cgcgccggaa ggtcccagcg cctcgcccgg
     6601 gaggaaggcc gactgcggcc cctcgacggc gacgacaccc cacccctcgg gagccatacg
     6661 ggcgaggagc ggaaagagcg tcgcggcgtg catgccgtag ccgtggaggc cgaggaggag
     6721 cgggaacggc ggcggcgcgt gcggcaggcg cgggaagagc tggagcggga cggagatcga
     6781 ggtcgtcgcg cgcggcggga ctccgcggcg ctccctcatc gggagtcccc gggtgggacg
     6841 aggctctcga gcgtcgtccc gcgcagcgcg gagaggaggg cgtcgcgggc gggaaggagc
     6901 gggggccccg ccggcccggc cgggacgtcg aggaggcgct cgaggtcgtc ggccccctcg
     6961 atcgcgagga ggacgtcggc gagcgtgatc tccgccgcgg gccgcccgag gacgaagccg
     7021 cccgtccggc cccgcttcga gcggaggacc cccgcgcggg cgagcttctg gagcaccttc
     7081 ccgagccacg gcgccggctc gccgcaggcg agagcgaggt cgcgcgcctc gcgccccggc
     7141 gcctcggggc cgcgcgggtc gaggtggggg agagccttca gggccgtctc tgcggcgcgg
     7201 gagagaagca ttggcgtcag cgacgggccg gcacggcgag cgagtcccgg gagcctcgcg
     7261 cgagcctcac gcgggcctca ctccccgacg acgaacgcgt actgctcggt cgtcagcggg
     7321 acgacggaga gccgtccgat cgtcaggaga ggcgacccgg cgaagagctt cgccgccttc
     7381 atctccgcga gcgtcacggg ccgcttcagc ggcttgccgg ccttcacgtc gacgaccgtt
     7441 cccttcgggt cggccgggtc ggggcgcggc g
//
LOCUS       DAOTEZ010000194         7416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_7082_length_7416_cov_7.212471, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000194 DAOTEZ010000000
VERSION     DAOTEZ010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7416
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7082_length_7416_cov_7.212471"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1640
                     /locus_tag="P5164_11160"
     CDS             <1..1640
                     /locus_tag="P5164_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRY44492.1"
                     /translation="VLTLIGSHLGENMHNTQVQDFAQMIAKGGQLVVVDPRFSTAAGK
                     ARYWLPIKPGTDMALLLAWIHVILDEGLWDRDYVAENAVGLDELRAHVADKTPEWAFT
                     ETTIPAATIAETARFIAGARPASLIHPGRHVTWYGDDVQRSRAIAILNALLGSWGRRG
                     GIFVPTQMPVPKYPVPEYAEKPKATQDAPTGTVYPFAEEVLSHGVCDATVPGRIGSPG
                     CPVKAWIVYGSNLLQALPQRKNVLEAIQNLALLVAVDVLPAEIVGYADVVLPEATYLE
                     RWDDIAPVPWSEPFLAVRQQVVPPMYESKPGWWIAKELGKRLGLAEYFPWESGEQLVT
                     ERLKKGGYDVEAIRKKGVVKGTPVPVRTEEGLQLAFGTPSKKIELYSKQMADAGLPPL
                     PPFTRHEQPADGQFRLLFGRSPVHTFGRTTNNRFLSEVYSENEVWLNAKAAREQGLAH
                     GDVVTLVNQDGVRSQPVKLKVTQRIRPDCVFVVHGYGHTNPGLKFAANRGMDDQTLVT
                     RIAVDPVAGTTGMSVNFVVIEKTEKAADASVAAKAEA"
     gene            1642..2199
                     /locus_tag="P5164_11165"
     CDS             1642..2199
                     /locus_tag="P5164_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRY44493.1"
                     /translation="MSASSPAAKRYAMTVDTRQCVGCKACVLACKAENAVPDGFCRQW
                     IVEEVRGEFPRLSAEIRSERCNHCTDAPCVKNCPTGASHVNEGGVVLVTHGKCSGCKA
                     CIAACPYDARFVHPEGYVDKCTFCLHRVQKGLPPACVGVCPTGTLTFGDTNDPESAVS
                     TLLRERRSKVNHPESGAGPNVHFLV"
     gene            2210..3178
                     /gene="nrfD"
                     /locus_tag="P5164_11170"
     CDS             2210..3178
                     /gene="nrfD"
                     /locus_tag="P5164_11170"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HRY44494.1"
                     /translation="MNELLEATTTRTNPGIDPTLHIWGWEIVVYLFLGGLVAGIFVLA
                     AALELRSGEKPKGDALRLMPFAALGLLSLGMVALWLDLAHKLWAWRFYLFFHATSPMS
                     WGAWILVLIYPVGLLLGLGSMSEAQRAWLRGKAPKALASLLEKGFAWADGGRRGILRT
                     SVGMGVALGIYTGLLLGTMPSRIAWNSAVLGPLFLASGISTGAALLLLLPLEEGERKT
                     LVRWDAAAIVAELVLVALMFLGWATSGDAGQWAAAQFLGGPYTAVFFSCVVVLGLLVP
                     LAMEAIEARRNVPFAVMLPVLILSGGLALRWILLTAGQHSAFRFLP"
     gene            3192..3713
                     /locus_tag="P5164_11175"
     CDS             3192..3713
                     /locus_tag="P5164_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRY44495.1"
                     /translation="MQPPVERPYWNPYVAGFALGLVLLGSFLVMGFGLGSSSVPTRLA
                     IGVAHAIAPAATEANAYFAQYVGPGKKLLEDWLVFEVLGVFLGGLVGAYSAGRLKSEV
                     IRGEGVPKGRRIALAIAGGVLMGFAARLARGCTSGQALTGGAVLSLGSWIFMMCVFAG
                     GYLLAPFLRKEWR"
     gene            3710..4897
                     /locus_tag="P5164_11180"
     CDS             3710..4897
                     /locus_tag="P5164_11180"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRY44496.1"
                     /translation="MNVLPLYPHDAFGFPTGLLLGTLIGFAFGFVLERAGFGNAKNLA
                     AQFYLTDTRVLKVMFSAIATACAGLALLSGLGVLDLSLVTVPETFLGPHVVGGLLLGA
                     GFMISAYCPGTGVVAMASGKWDGFWSIVGVMAGSLLFGFLYGPLEGFYKSGAMGSVTI
                     SQATGIPFPVLAAGVVAMAIGAFLGAEKLEAIFAPKMGKAVPAANSSLRNRQFATLGT
                     LAALGLVAWVAVPRVRPVAAAKPFARIDPLDLAKEVVSDPQQLWIVDLRDPKAVAPAE
                     RIPGTATLPEGTKPAAFAATLPPTRTLVVYAQGDVAALPAGFEAFPGEVLALAGGFDA
                     WKREVLATPQPPADPTPALVAEFRTRSALNGYFTGAAAAPPPKLVVKAVAPSAAPKKG
                     GGC"
     gene            4992..6245
                     /locus_tag="P5164_11185"
     CDS             4992..6245
                     /locus_tag="P5164_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HRY44497.1"
                     /translation="MRRNPALAITALALLALAAPAFATNGYFSLGYGTPYKGMAGAGQ
                     ALHLNTLASATNPATIAFVKGYDVSLALFNPNREYTVTGNPSGYPGTFGLAPGSYESD
                     SRAFVMPAMAGAWHVSERFHVGLAIYGNGGMNTDWSNPVFGQAPAGVNLMQAFIAPSF
                     TWEFAEHQAIGVSPILGWQSFEAKGLSAFSMFSSNPAKLTNNGADTTTGFGARIGYHG
                     KFGEYLGVGASYQTKIAMGEFDDYAGLFAGKGDFDVPATWTAGIAVFPAKGWSIAADV
                     QQIYYSEVGSVGNPMLPNLMQAPLGADGGAGFGWDDVTVYKLGVQWEATPAWTLRAGY
                     AHCTQPVQESEVLFNILAPGIVQDHVTVGVSKLLSPKSALHVAVTRAFSQTVSGANPL
                     EVPGQQTIELKMDQWEVELGWGFGF"
     gene            6334..6540
                     /locus_tag="P5164_11190"
     CDS             6334..6540
                     /locus_tag="P5164_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HRY44498.1"
                     /translation="MPGVYKKVELVGTSPVSFAEAVKAAVEEASKTVRHMDWFEVVEE
                     RGRIHDGKVAEFQVTIRVGFKLER"
     gene            complement(6616..>7416)
                     /locus_tag="P5164_11195"
     CDS             complement(6616..>7416)
                     /locus_tag="P5164_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HRY44499.1"
                     /translation="VGMHYFSPVPKMPLLEVVAPKGAAPWAVATARAFGVAQGKTVIV
                     VKDGPGFYTTRILAPFVNEAVVLLDEGAAIPDLDAALLDFGFPVGPVALLDEVGIDVA
                     AHVARDLGKAFASRGAAASGSFARLLGAGFRGRKNGKGFYRYGPSARPGGRKEVDRDV
                     YAHFGGTERTPMRAADLADRLVLLMVNEAAWCLQEEVVASPRDADAGAVLGLGFPPFL
                     GGPFRWLDAVGAAEAVARMERLRRLHGKRFTPAPLLVEAARTGRPFHG"
BASE COUNT         1033 a         2798 c         2487 g         1098 t
ORIGIN      
        1 gggtcctcac gctcatcggc tcgcacctcg gcgagaacat gcacaacacg caggtgcagg
       61 acttcgcgca gatgatcgcg aaggggggcc agctcgtcgt cgtcgacccc cgcttctcga
      121 cggccgcggg caaggcccgg tactggctcc cgatcaagcc cgggaccgac atggcgcttc
      181 tcctcgcctg gatccacgtc atcctcgacg agggcctctg ggaccgcgac tacgtcgccg
      241 agaacgccgt cggcctcgac gagctgaggg cgcacgtcgc ggacaagacc cccgagtggg
      301 ccttcaccga gacgacgatc ccggccgcga cgatcgccga aacggcgcgc ttcatcgcgg
      361 gcgcccggcc cgcctcgctg atccaccccg gccggcacgt cacctggtac ggcgacgacg
      421 tccagcgctc gcgcgccatc gcgatcctca acgcgctcct cgggtcgtgg ggacgccggg
      481 gcgggatctt cgtcccgacg cagatgccgg tcccgaagta cccggtcccc gagtacgccg
      541 agaagccgaa ggcgacgcag gacgccccga ccggaaccgt ctacccgttc gccgaggagg
      601 tcctctcgca cggcgtgtgc gacgcgacgg tgcccgggcg gatcgggagc cccggctgtc
      661 ccgtcaaggc gtggatcgtc tacggctcga acctcctcca ggcgctgccg cagcggaaga
      721 acgtcctgga ggcgatccag aacctcgccc tcctcgtcgc ggtcgacgtc ctcccggccg
      781 agatcgtcgg ctacgccgac gtcgtcctcc ccgaggcgac ctacctcgaa cgctgggacg
      841 acatcgcccc cgtcccgtgg tccgagccgt tcctcgccgt ccgccagcag gtcgtcccgc
      901 cgatgtacga gtcgaagccg ggctggtgga tcgcgaagga gctcgggaag aggctcggcc
      961 tcgcggaata cttcccctgg gagagcggcg agcagctcgt caccgagcgg ctgaagaagg
     1021 gcggctacga cgtcgaggcg atccggaaga agggggtcgt caaggggacg cccgtccccg
     1081 tgaggaccga ggagggcctt cagctcgcct tcgggacccc ctcgaagaag atcgagctct
     1141 actcgaagca gatggccgac gcgggcctgc cgcccctgcc ccccttcacg cggcacgagc
     1201 agccggccga cgggcagttc cggctcctct tcgggaggtc gcccgtccac acgttcggcc
     1261 ggacgacgaa caaccgcttc ctctccgagg tctactccga gaacgaggtc tggctgaacg
     1321 cgaaggccgc gcgggagcag gggctcgcgc acggcgacgt cgtgacgctc gtgaaccagg
     1381 acggcgtccg ttcgcagccc gtgaagctca aggtgacaca gcggatccgc cccgactgcg
     1441 tcttcgtcgt ccacggctac ggccacacga acccggggct gaagttcgcg gcgaaccgcg
     1501 ggatggacga ccagacgctc gtgacccgga tcgccgtcga cccggtcgcc gggacgacgg
     1561 ggatgagcgt caacttcgtg gtcatcgaga agaccgagaa ggccgccgac gcgagcgtcg
     1621 cggcgaaggc ggaggcctga gatgagcgcg tcgagccccg ctgcgaagcg gtacgcgatg
     1681 acggtcgaca cccggcagtg cgtcgggtgc aaggcgtgcg tcctcgcctg caaggccgag
     1741 aacgccgtcc ccgacggctt ctgccggcag tggatcgtcg aggaggtccg gggcgagttc
     1801 ccgcgcctct cggccgagat ccgctccgag cgctgcaacc actgcaccga tgctccttgt
     1861 gtcaagaact gcccgacggg cgcctcccac gtgaacgagg ggggcgtcgt cctcgtgacg
     1921 cacgggaaat gctcgggctg caaggcgtgc atcgcggcct gcccgtacga cgcccgcttc
     1981 gtccaccccg aggggtacgt cgacaagtgc accttctgcc tccaccgggt gcagaaaggg
     2041 ctcccgcccg cctgcgtcgg cgtctgcccg accggcaccc tcaccttcgg cgatacgaac
     2101 gacccggagt cggccgtctc gacgctcctc cgcgagcggc gctcgaaggt gaaccacccc
     2161 gagtcgggcg ccggcccgaa cgtccacttc ctcgtctgag gcgctgtcga tgaacgagct
     2221 tctcgaagcc accaccaccc ggacgaaccc cggcatcgac ccgacgctcc acatctgggg
     2281 ctgggagatc gtcgtctacc tcttcctcgg cggcctcgtc gccgggatct tcgtcctcgc
     2341 tgccgctctc gagctccgct cgggcgagaa gccgaagggc gacgcgctgc ggctcatgcc
     2401 gttcgccgcc ctcgggctcc tctccctcgg catggtcgcc ctctggctcg acctcgccca
     2461 caagctctgg gcctggcgct tctacctctt cttccacgcc acctcgccga tgtcgtgggg
     2521 cgcctggatc ctcgtcctga tctaccccgt cggcctcctc ctggggctcg ggtcgatgag
     2581 cgaggcgcag cgcgcgtggc tccgcggaaa ggcgccgaag gcgctcgctt ctctcctcga
     2641 gaagggcttc gcgtgggcag acggcggacg gcgcgggatc ctccggacga gcgtcgggat
     2701 gggggtcgcg ctcgggatct acaccggcct cctcctcggg acgatgccct cgcggatcgc
     2761 ctggaacagc gccgtcctcg gccccctctt cctcgcctcg gggatctcga ccggcgccgc
     2821 gctcctcctc ctcctcccgc tcgaggaggg cgagaggaag acgctcgtga ggtgggacgc
     2881 cgccgccatc gtcgccgagc tcgtcctcgt cgccctgatg ttcctcggct gggcgacctc
     2941 gggcgacgcc gggcagtggg ccgccgcgca gttcctcggc gggccgtaca cggccgtctt
     3001 cttctcctgc gtcgtcgtcc tcggcctcct cgtcccgctc gcgatggagg cgatcgaggc
     3061 gaggaggaac gtcccgttcg ccgtgatgct cccggtcctg atcctctccg gcgggctcgc
     3121 gctccgctgg atcctcctga cggcggggca gcactccgcc ttccgcttcc tcccgtgacg
     3181 aggtgaacgc gatgcagccc cccgtcgagc gcccctactg gaacccctac gtggccggct
     3241 tcgccctcgg tctcgtcctc ctcggctcgt tcctcgtgat ggggttcggg ctcggctcct
     3301 cgagcgtccc gacgcggctc gcgatcggcg tcgcccacgc gatcgccccg gcggcgaccg
     3361 aggcgaacgc ctacttcgcc cagtacgtcg gccccggcaa gaagctcctc gaggactggc
     3421 tcgtcttcga ggtcctcggc gtcttcctcg gcggcctcgt cggcgcctac tccgccggcc
     3481 gcctgaagag cgaggtgatc cgcggcgagg gggtcccgaa gggacgccgc atcgcgctcg
     3541 cgatcgccgg cggcgtcctg atgggcttcg cggcccgcct cgcccgcggc tgcacttccg
     3601 gccaggcgct cacgggcggc gcggtcctct cgctcggctc ctggatcttc atgatgtgcg
     3661 tcttcgcggg cggctacctc ctcgccccgt tcctcaggaa ggagtggcga tgaacgtcct
     3721 gccgctctac ccgcacgacg ccttcggctt cccgacgggc ctcctcctcg gcacgctcat
     3781 cggcttcgcg ttcggcttcg tcctcgagcg cgccggcttc ggcaacgcga agaacctcgc
     3841 cgcgcagttc tacctgacgg acacgcgcgt cctgaaggtg atgttcagcg cgatcgccac
     3901 ggcctgcgcc ggcctcgcgc tcctctcggg cctcggcgtc ctcgacctct cgctcgtcac
     3961 cgtccccgag acgttcctgg ggccgcacgt cgtcggcggc ctcctcctcg gcgccggctt
     4021 catgatctcg gcctactgcc ccggcaccgg cgtcgtcgcg atggcctcgg gcaagtggga
     4081 cggcttctgg tcgatcgtcg gcgtcatggc cggctcgctc ctcttcggct tcctctatgg
     4141 cccgctcgag gggttctaca agagcggagc gatggggtcg gtgacgatct cgcaggcgac
     4201 ggggatcccg ttcccggtcc tcgccgcggg ggtcgtcgcg atggcgatcg gcgcctttct
     4261 cggcgcggag aagctggagg cgatctttgc cccgaagatg ggcaaggccg tacctgccgc
     4321 gaactcctcc cttcgcaacc gccagttcgc cacgctcggc acgcttgccg cgctcgggct
     4381 cgtcgcctgg gtcgccgtcc cgcgggtccg tcccgtcgcc gcggcaaagc ccttcgcccg
     4441 gatcgacccc ctcgacctcg cgaaggaggt cgtctccgac ccgcagcagc tctggatcgt
     4501 cgacctccgc gacccgaagg ccgtcgcgcc cgccgagcgg atccccggga cggcgacgct
     4561 ccccgagggg acgaagcccg ccgccttcgc cgcgacgctc cccccgacgc ggacgctcgt
     4621 cgtctacgcg cagggcgacg tcgcggcgct gccggcgggc ttcgaggcct tcccgggcga
     4681 ggtcctcgcg ctcgcgggcg ggttcgacgc ctggaagcga gaggtcctcg cgacgccgca
     4741 gcctccggcc gacccgaccc cggcgctcgt cgccgagttc cggacgaggt cagcgctgaa
     4801 cggctacttc acgggcgcgg cggcggcccc gccgccgaag ctcgtcgtga aggccgtcgc
     4861 cccctccgcc gcgccgaaga agggcggcgg ctgctgacac gctgaccccg gcccgagggc
     4921 cggttcccga ttccgaccac agacccgagt tccggcgagc cccgaagggg cctgcccatg
     4981 gagagtgtgc gatgcgacga aaccccgctc tggcgatcac ggccctggcc ctgctcgccc
     5041 tggccgctcc cgcgttcgcg acgaacggct acttctccct cggctacggc acgccgtaca
     5101 aggggatggc cggcgccggc caggcgctcc acctgaacac gctggcgagc gccacgaacc
     5161 ccgcgacgat cgcgttcgtc aagggctacg acgtcagcct cgcgctcttc aacccgaacc
     5221 gcgagtacac cgtcaccggg aacccctccg gctaccccgg cacgttcggc ctcgcgcccg
     5281 ggagctacga gagcgacagc agggccttcg tcatgccggc gatggcgggc gcgtggcacg
     5341 tctccgagcg cttccacgtc ggactcgcga tctacgggaa cggcgggatg aacaccgact
     5401 ggtcgaaccc cgtcttcggc caggcccccg ccggcgtcaa cctgatgcag gccttcatcg
     5461 ccccctcctt cacgtgggag ttcgcggagc accaggcgat cggcgtctcg ccgatcctcg
     5521 gctggcagag cttcgaggcg aagggactct cggccttctc gatgttctcg tcgaaccccg
     5581 cgaagctgac gaacaacggc gccgacacca cgaccggctt cggcgcgcgg atcggctacc
     5641 acggcaagtt cggggagtac ctcggcgtcg gcgcctccta ccagacgaag atcgccatgg
     5701 gcgagttcga cgactacgcg ggcctcttcg ccggcaaggg cgacttcgac gtccccgcga
     5761 cctggacggc cggcatcgcc gtcttcccgg cgaaggggtg gtcgatcgcc gccgacgtcc
     5821 agcagatcta ctacagcgag gtcggctccg tcgggaaccc gatgctcccg aacctgatgc
     5881 aggccccgct cggcgccgac ggcggcgccg gcttcggctg ggacgacgtc accgtctaca
     5941 agctcggcgt ccagtgggag gcgactcctg cgtggacgct ccgcgccggc tacgcccact
     6001 gcacccagcc ggtgcaggag agcgaggtcc tattcaacat cctcgccccg ggcatcgtcc
     6061 aggaccacgt caccgtcggc gtctcgaagc tcctctcgcc gaagagcgcc ctccacgtcg
     6121 ccgtgacccg cgccttctcg cagaccgtca gcggcgcaaa cccgctcgag gtcccgggcc
     6181 agcagacgat cgagctgaag atggaccagt gggaggtcga gctcggctgg gggttcggct
     6241 tctgacgcct tccgcgcggc gggcggcgct ccgcccgccc cgctccgggc cgatagaatc
     6301 cccgcgaacc ccacgattcg cgaggaggaa ccgatgcccg gtgtctacaa gaaggtcgag
     6361 ctcgtcggca cgtcgcccgt cagcttcgcc gaggccgtga aggccgccgt cgaggaagcg
     6421 tcgaagacgg tccggcacat ggactggttc gaggtcgtcg aggagcgagg ccgaatccac
     6481 gacgggaagg tcgccgagtt ccaggtgacg attcgcgtcg gcttcaagct cgagcgctga
     6541 gcttcgcggg gcgggccgga aggcccgccc cgccgcatct cccgtcgttt cgcagccggt
     6601 ccgtctccgc ggcggtcagc cgtggaacgg ccggccggtc cgcgccgcct cgacgaggag
     6661 cggagccggc gtgaagcgct tgccgtggag ccgtcggagg cgctccatcc tcgcgacggc
     6721 ctccgccgcg ccaacggcgt cgagccaccg gaacgggccg ccgaggaacg gcgggaaccc
     6781 gaggcccagg acggcgcccg cgtccgcgtc gcgcggcgag gcgacgacct cctcctggag
     6841 gcaccaggcc gcctcgttga ccatcaggag gacgagccgg tcggcgaggt cggcggcgcg
     6901 catcggcgtc cgctccgtcc cgccgaagtg cgcgtagacg tcgcggtcga cctccttccg
     6961 tccgccgggc cgcgcggacg gcccgtaccg gtagaacccc ttcccgttct tccgcccgcg
     7021 gaagcccgcg ccgaggagcc gcgcgaacga gcccgacgcg gcggcgcccc gcgaggcgaa
     7081 ggccttcccg aggtcgcgcg cgacgtgcgc cgcgacgtcg atcccgacct cgtcgaggag
     7141 cgcgacgggt ccgaccggaa acccgaagtc gaggagcgcc gcgtcgaggt ccgggatcgc
     7201 cgccccttcg tcgaggagga cgaccgcctc gttcacgaac ggcgccagta tccgagtcgt
     7261 gtagaagccg gggccgtcct tcacgacgat caccgtcttc ccctgcgcga caccgaacgc
     7321 ccgcgccgtc gcgacggccc acggggcggc gcccttcggt gcgacgacct cgaggagcgg
     7381 catcttcggg acgggcgaga agtagtgcat cccgac
//
LOCUS       DAOTEZ010000195         7398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_7114_length_7398_cov_3.671932, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000195 DAOTEZ010000000
VERSION     DAOTEZ010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7398
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7114_length_7398_cov_3.671932"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..681)
                     /locus_tag="P5164_11200"
     CDS             complement(1..681)
                     /locus_tag="P5164_11200"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRY44500.1"
                     /translation="MALNPISFTEKVVSSFLRYQLTAYRFADSRLHAQMRELLSLEKT
                     RATPLLKGPYVSVSRGFRAGATLAELAAEGVVHPALAGLAPFPSVWAHQEAAIRAIRS
                     GRTTVVSTGTGSGKTEAFLYPIISRCLELRDAGAAPGIVAVLVYPMNALAEDQLGRLR
                     DLLSGTGVPFGMYVGKTPERAADVSGVRLPKGSSREEYRRRLAKLRDEKRNEAVHPPI
                     LSRKYASW"
     gene            complement(793..1029)
                     /locus_tag="P5164_11205"
     CDS             complement(793..1029)
                     /locus_tag="P5164_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRY44501.1"
                     /translation="MRSITSVVPGPDFTLRVAFDDGEARSFDLKPWLADEAFEELADP
                     DEFRKVRSQGYFVEWENGADLSADTLYLLGREEG"
     gene            complement(1029..1298)
                     /locus_tag="P5164_11210"
     CDS             complement(1029..1298)
                     /locus_tag="P5164_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019987264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HRY44502.1"
                     /translation="MPVISMFFGIVIRMFYRDDQRHHLPHIHAEYQGEVGVFSIVDGS
                     LLEGSLPQGKRKLVEAWIEVRRDELLADWSLAVDGQRVFRIRGLE"
     gene            1389..5120
                     /locus_tag="P5164_11215"
     CDS             1389..5120
                     /locus_tag="P5164_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44503.1"
                     /translation="MPKKRASRLPDPEDVLAGRVRVRADELFALVHDVNPTGEESPRE
                     RERYALKSRLQGLLLTRFGDEVEVVPDPTNPDLVSLRHRSGLRDACHALVSQLPVEAR
                     ALVRRRLDAGEGGMEAAGPAATRTSAGRSDPPAKRSTAAEHDAPDDAASLEARGLAAL
                     DEYDYEEAQRLLTAAVERRAGPVAARALVELLVDVLADDAAALGLEGSLDPASRADPG
                     VRGFLALAAARSGDVDRASRLVRGLDGPLPARVYATLSRAALGAGDLSSAATHLSAVR
                     VADPAFPEAAELEARLERARRDERRPAEEALLALHASGDLDAAESAARAFLARWPDGA
                     AGRRVLREIEDARRRERAASLARDGAAALGRCDFAEAARLLGLALAADPALPGGPALL
                     DRARRGTAEAADEDAVRRVVEALASGPSLEALGAFAELPAALRARVRTVMKAPEVALA
                     DEVLAASPAEKPRAAADAALALAAAERAFHGGDAAEALRLVESHTRALGRLPRALALE
                     REARAALAAARAAAACASLDPVRAALERGDLDAAAALLGRVRKTELDAEGRATLATLA
                     GRLRQDREARHASHDAEAREAARKALQLAVSDETGPADELADLARDFDLTRTRPWLSG
                     DGRRLVLVETAAGWLFVRVLDVERQEVVRRISLRPPHLIGPFETGLVEGSRIRVVGEE
                     LGLVDLDFATTEVVRAVSLAGARPPSSVVEETLPLPSSDFLWLEVTNGPNREPCSYLV
                     DAASGRTRAKLPFDPSPSVVFREGASVLVTADERRARLLTLDGLPAAGDLPTLPFHLE
                     TAGPDPTGPGILLAGRAPTRPGTDEDAPAPLRVLELQPGPTSGFGRHVDLPGSEGELD
                     VGLATSRSEALAFALCPTRAESRVYTIGPGLDLSVPFVTTPEETVLFVDAGSRHVVAG
                     CWWGERFAAVPLNRETRWPEWKGSLRARDPLPRGTLLGESYLCETRSRIANAHSLALY
                     SEISGLDGERLDKRVGEIMARAATGDQHEALLGALERMGPRYELRQRVETDLLARFPL
                     HPLAVLTALRRHANETRWERLRDDARAFRRGKPPHVPPHVLHLEALALARLGELDEAL
                     ALVEELRRRKDEGACRVDALRTVLKECLAKRPSSSRLGRLVTLVRRALAAHAAGGSAL
                     VAVLLDRPLVRASGIYQAHALLAAARLDAKADTPRALFRKRLALARLLQIHEERPLQR
                     RDLPLLPGALDDAAVDAIAARAREFLERMDEEGEAPAPA"
     gene            complement(5123..5542)
                     /locus_tag="P5164_11220"
     CDS             complement(5123..5542)
                     /locus_tag="P5164_11220"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRY44504.1"
                     /translation="MVRRDVVAVRVARAAAWLDDVERAFAGPVETLLADVKTRDLALF
                     YLFLAIQECIDLAAHWVADEGWSPADDAGSTFDVLAGRGVIDLETAEALRSAAGLRNR
                     IAHGYALLDARRVRDEARQGLPSLRAFLAAAAGAAGL"
     gene            complement(5529..5933)
                     /locus_tag="P5164_11225"
     CDS             complement(5529..5933)
                     /locus_tag="P5164_11225"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRY44505.1"
                     /translation="MPPIELLSRVLADVPAVRLAVLFGSAARGQAGPRSDLDVGVDLE
                     GGPEALSRLAVRLCRETAREVDLVSLREAPPLLRFEIARDGVVLVERAPHAWADFRAR
                     AMTDWWDWAPTARIMHAAALGRLREGLARGAA"
     gene            complement(6082..6387)
                     /locus_tag="P5164_11230"
     CDS             complement(6082..6387)
                     /locus_tag="P5164_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HRY44506.1"
                     /translation="MAARTKLLEPIPPGEILMEEFMRPLGLSINSLARALSVPANRIS
                     GIVNGTRAISADTALRLGKYFGTSPEIWLDLQSDYELRVARETTWKEVEKRVQTRVA"
     gene            complement(6397..6675)
                     /locus_tag="P5164_11235"
     CDS             complement(6397..6675)
                     /locus_tag="P5164_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRY44507.1"
                     /translation="MIRSFKSKEAEALFHDLPVRRFRAIERPARRKLLYLHRARVLED
                     LKAPPGNQLEALKGDRKGQHSIRVNDQWRICFIWKDGDAYGVEIVDYH"
     gene            complement(6804..>7398)
                     /locus_tag="P5164_11240"
     CDS             complement(6804..>7398)
                     /locus_tag="P5164_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44508.1"
                     /translation="TSAVFSAAWLAVAKSDEGAAIGGRPWRSLWAITPHERLRLRALL
                     DAVVAELYGLDFDDLAWILRDCDHPAALLREKAFCRTLDPKGFWRVDKEKDPELRHTV
                     LSLAAFRDLKQTIAAHGGDREKGIAAYCDAHDGDGWMLPETLRLADLGLGHDDRAREA
                     QPVASRLGERFLPWQLAQSVEESWAECERHAERLRRG"
BASE COUNT          987 a         2896 c         2549 g          966 t
ORIGIN      
        1 tcaccagctt gcgtacttcc tggacaatat gggcgggtgg acggcttcgt tccgtttctc
       61 atcgcggagc ttggcgagcc gccgccggta ctcctcccgc gaggacccct tcggcagccg
      121 gactccggag acgtcggccg cccgctccgg cgtcttcccg acgtacatcc cgaacggcac
      181 ccccgtcccc gagaggaggt cccggaggcg cccgagctgg tcctcggcga gggcgttcat
      241 cgggtagacg aggacggcga cgatccccgg cgccgcaccc gcgtcgcgca gctccaggca
      301 gcggctgatg atcgggtaga ggaacgcctc cgtcttcccg gagcccgtcc ccgtcgagac
      361 gaccgtcgtc cggcccgagc ggatcgcccg gatcgccgcc tcctggtgcg cccagaccga
      421 cgggaacggc gcgaggcccg cgagcgccgg gtggacgacg ccttccgccg ccagctccgc
      481 gagcgtcgcg cccgcccgga acccccgcga cacggagacg tacggcccct tcaacagcgg
      541 cgtcgcccgc gtcttctcca gcgagaggag ctcgcgcatc tgtgcgtgca gccgcgaatc
      601 cgcgaagcgg tacgccgtca gctggtagcg caggaagctc gagacgacct tctcggtgaa
      661 cgagatcggg ttcagggcca tccggtcacc tcagcgacga ggtccgccgc cgtcgcggtc
      721 accaccgcgg gcgacgggcg cgtgcgctcc cggcgacaat cgcgggtcgc gccagcccgc
      781 atctcttccg cttcagccct cttcccggcc gaggaggtag agcgtgtcgg cgctcaggtc
      841 ggcgccgttc tcccactcca cgaaatagcc ctgcgagcgg accttgcgga actcgtccgg
      901 gtccgccagc tcctcgaacg cctcgtcggc gagccacggc ttcaggtcga aagagcgcgc
      961 ctcgccgtcg tcgaacgcga cgcgcagcgt gaagtccgga ccgggcacga cactcgtgat
     1021 cgacctcatc actcgagccc ccggatgcgg aagacgcgct gaccgtcgac cgcgaggctc
     1081 cagtccgcca gcagctcgtc ccgtcggacc tcgatccatg cctccacgag cttcctcttc
     1141 ccttgcggaa gcgaaccctc gagcagggac ccgtcgacga tcgagaagac cccgacctcg
     1201 ccctggtatt cggcgtggat gtgcggcagg tgatgccgct ggtcgtccct gtagaacatc
     1261 cgaatgacga tgccgaagaa catcgagatg acgggcattc ggcgactccg gaggcgagta
     1321 tagccaccgg gcacgcccgg gccgtcgaac cgcctgctgc gagaatccgc cggaaagagc
     1381 cccccgccat gccgaagaag cgcgcctccc gcctccccga ccccgaggac gtcctcgcgg
     1441 ggcgggtccg cgtccgcgcc gacgagctct tcgccctcgt ccacgacgtc aacccgacgg
     1501 gcgaggagag cccgcgcgag agggagcgct acgcgctgaa gtcgcgcctc caggggctcc
     1561 tcctgacccg cttcggagac gaggtcgagg tcgtccccga ccccacgaac cccgacctcg
     1621 tgagcctgcg ccatcgctcg ggcctgcgcg acgcctgcca cgccctcgtc tcgcagctcc
     1681 cggtcgaggc gcgcgccctc gtgcggcggc ggctcgacgc gggcgaaggc ggcatggagg
     1741 ctgccggccc cgccgcgacc cgcacgtccg cggggcggag cgacccgccg gccaagagga
     1801 gcaccgccgc cgaacacgac gccccggacg acgccgcctc cctcgaagcc cgcggcctcg
     1861 ccgcgctcga tgagtacgac tacgaggaag cgcagcgcct cctcacggcc gccgtcgagc
     1921 gccgcgcggg ccccgtcgcc gcccgcgccc tcgtcgagct cctcgtcgac gtcctcgccg
     1981 acgacgcggc cgctctcggg ctcgagggct cgctcgaccc ggcgtcccgc gccgacccgg
     2041 gcgtccgcgg attcctcgcc ctcgccgcag cccggtcggg cgacgtcgac cgcgcgagcc
     2101 gcctcgtgcg cggcctcgac ggcccgctcc cggcccgggt ctacgcgacc ctctcccgtg
     2161 ccgcgctcgg cgcgggcgac ctctcctcgg ccgcgaccca cctctccgcc gtccgcgtgg
     2221 ccgaccccgc gttcccggag gcggcggagc tcgaggctcg cctcgaacgc gcgcgacgcg
     2281 acgagagaag gcccgcagag gaggcgctcc tcgcccttca cgcctccggc gacctcgacg
     2341 cggccgagtc ggcggcccgc gcgttcctcg cccgctggcc cgacggcgcc gcgggccggc
     2401 gcgtcctccg cgagatcgag gacgcccgcc gccgcgagcg cgccgcatcg ctcgcacgag
     2461 acggcgccgc cgccctcgga cgctgcgact tcgccgaagc ggcccgcctc ctcggcctcg
     2521 cgctcgcggc cgaccccgcg ctcccgggcg ggcccgccct cctcgaccgt gcccggcgcg
     2581 ggaccgccga ggcggccgac gaggacgccg tgcgccgggt cgtcgaagcc ctggcctccg
     2641 gcccctcgct cgaggcgctc ggcgcgttcg ccgagctgcc ggccgcgctc cgcgcgcggg
     2701 tgcggaccgt gatgaaagct cccgaggtcg cgctcgccga cgaggtcctc gccgcctccc
     2761 cggccgagaa gccccgcgcc gcagcggacg ccgccctcgc tctcgccgcg gccgagcggg
     2821 ccttccacgg gggcgacgcg gccgaggcgc tgcgcctcgt cgagagccac acccgggcgc
     2881 tcggccgcct tccgagagcg ctcgccctcg aaagggaggc gcgcgcggcg ctcgccgcgg
     2941 cgcgtgccgc cgccgcttgc gcgtccctcg acccggtccg cgccgcgctc gagcgcggcg
     3001 acctcgacgc cgccgcggcc ctcctcggca gggtgaggaa gacggagctc gacgccgagg
     3061 ggcgcgccac cctcgccacg ctggccgggc gccttcgcca ggaccgcgag gcgcgccatg
     3121 cctcccacga cgccgaagcc cgcgaggccg cccgcaaggc ccttcagctc gccgtctccg
     3181 acgagacagg ccccgccgac gagctcgccg acctcgcccg cgacttcgac ctcacgcgga
     3241 cccgcccctg gctctccggc gacggccgcc gcctcgtcct cgtggagacc gccgccggct
     3301 ggctcttcgt ccgggtcctc gacgtcgaac ggcaggaggt cgtccggcgg atctcgcttc
     3361 ggccccctca cctgatcggg ccgttcgaga ccgggctcgt cgaggggagc cggattcgcg
     3421 tcgtcggcga ggagctgggg ctcgtcgacc tcgacttcgc cacgacggag gtcgttcggg
     3481 ccgtctcgct cgccggcgcc cgccccccct cgtccgtcgt cgaggagacg cttccgctgc
     3541 cttcgtcgga cttcctctgg ctcgaggtga cgaacggtcc gaatcgggag ccgtgctcgt
     3601 acctcgtcga cgccgcctcc gggcgaaccc gcgcgaagct ccccttcgac ccgtcgccct
     3661 ccgtcgtctt ccgcgagggc gcctccgtcc tcgtcaccgc ggacgagcgg cgcgcgcggc
     3721 tcctcaccct cgacggcctt cccgcggccg gcgaccttcc gaccctcccg ttccacctcg
     3781 agaccgcagg ccccgacccg accggccccg gcatccttct cgcggggcgc gccccgaccc
     3841 ggccagggac ggacgaagac gcgcccgccc cgctccgcgt cctcgagctc cagcccggcc
     3901 cgacgagcgg tttcgggcgc cacgtcgacc tgccgggcag cgagggcgag ctcgacgtcg
     3961 gcctcgcgac gtcccggagc gaagcgctcg ccttcgccct ctgccccacc cgcgcggaga
     4021 gccgcgtcta cacgatcggc ccgggactcg acctgtccgt cccgttcgtg accactcccg
     4081 aggagacggt cctcttcgtc gacgcaggct cgcgacacgt cgtcgccggc tgctggtggg
     4141 gagagcgctt cgcggccgtc ccgctgaaca gggagacgcg ttggcccgag tggaagggct
     4201 ccctgcgtgc ccgagacccg cttccgagag ggacgctcct cggcgagagc tacctctgcg
     4261 agacgcggtc ccggatcgcc aacgctcatt ccctcgcgct ctactcggag atcagcggac
     4321 tggacgggga acgcctcgac aagcgcgtcg gcgagatcat ggcgcgtgcc gccaccgggg
     4381 atcagcacga ggcgctcctc ggggcgctcg agaggatggg gccgaggtac gagctgaggc
     4441 agcgggttga aaccgacctc ctcgcgcgat tcccgctcca tcccctcgcg gtcctgacgg
     4501 cccttcgccg tcacgcgaac gagacgcgat gggagcgcct ccgcgacgac gcacgcgcct
     4561 tccgacgggg caagccccct cacgtccccc cgcacgtcct gcacctcgaa gcgctcgccc
     4621 tcgcccggct cggagagctc gacgaggccc tcgccctcgt cgaggagctc cggcgccgaa
     4681 aggacgaggg cgcctgtcgc gtcgacgccc tgcgcaccgt cctgaaggag tgcctcgcga
     4741 agaggccgtc ctcctcacgg ctcggccggc tcgtcaccct cgtaaggcgg gcgctcgccg
     4801 cgcacgcggc gggcgggtcc gcgctcgtcg ccgtcctcct cgaccgcccc ctcgtccggg
     4861 cctcgggcat ctaccaggcg cacgcccttc tcgccgccgc ccgcctcgac gcgaaggccg
     4921 acaccccgcg ggcgctcttc cgcaagcgtc tcgccctggc ccgcctcctc cagatccacg
     4981 aggagcgccc gctccagcgg cgcgacctcc ccctccttcc cggcgcgctc gacgacgcgg
     5041 ccgtcgacgc gatcgcggca cgtgcgaggg agttcctgga acggatggac gaagagggag
     5101 aggcgcccgc gccggcctga cgtcagagcc cggcggcgcc ggccgcggcg gcgaggaacg
     5161 cccgaagcga cgggagcccc tgccgcgcct cgtcccggac ccggcgcgcg tcgaggagcg
     5221 cgtacccgtg cgcgatccgg ttcctgagcc cggccgccga tcggagcgcc tcggccgtct
     5281 ccaggtcgat cacgcctcgt ccggcgagga cgtcgaaggt cgagcccgcg tcgtccgcgg
     5341 ggctccagcc ctcgtcggcc acccagtgcg ccgccaggtc gatgcactcc tggatcgcga
     5401 ggaagaggta gaagagcgca aggtcccgcg tcttcacgtc ggcgaggagc gtctcgacgg
     5461 gtccggcgaa ggctctttcg acgtcgtcga gccaggccgc ggcccgcgcg acgcggaccg
     5521 cgacgacgtc acgccgcacc acgagccagg ccctccctga gccgtccgag cgccgcggcg
     5581 tgcatgatcc gcgcggtcgg cgcccagtcc caccagtccg tcatcgcccg cgcccggaag
     5641 tcggcccagg cgtgcggggc tcgctcgacg agcacgactc cgtcgcgcgc gatctcgaac
     5701 ctcagcaggg gcggcgcctc gcggagcgag acgaggtcga cctcgcgggc cgtctcccgg
     5761 cagaggcgaa cggcgagccg ggaaagcgcc tcggggccgc cttcaaggtc cacgccgacg
     5821 tccaggtcgc tccgcggacc cgcctgcccg cgcgccgccg agccgaagag aacggcgagg
     5881 cgaacggcgg ggacgtccgc gaggacgcgg gagaggagct cgatcggggg catcgacgaa
     5941 atcgtagcac ccggcgatca gcgggacggt cgacgattcc catcgccgtt gggccgggcg
     6001 cctggcgggc acgtcgcggg gacgccgacg ggagcccgaa ggcaccttga aattccggcg
     6061 gtcaccgccg ggatttcaag gttacgcgac tcgcgtctgg acccgtttct cgacctcctt
     6121 ccaggtcgtc tcccgcgcga cgcgaagctc gtagtccgac tgaaggtcga gccagatctc
     6181 gggcgaagtg ccgaagtact tgcccagccg caaagcggtg tcggccgaga tcgcgcgggt
     6241 gccgttgacg atgccgctga tccggttcgc cgggacggag agcgctcgcg ccagcgagtt
     6301 gatgctgagc cccagcgggc gcatgaactc ctccatgagg atctcgcccg ggggaatcgg
     6361 ttccaggagc ttcgttctcg ccgccatggg tccctctcag tggtaatcca cgatctcgac
     6421 gccgtaggcg tcgccgtcct tccagatgaa acagattcgc cactggtcgt tgacgcgaat
     6481 gctgtgttgg ccctttcggt cgcccttgag cgcctcgagc tggttgcccg gcggggcctt
     6541 caagtcctcc agcacgcgag cccggtgaag atagaggagc ttccgtcgcg cgggtcgctc
     6601 gatcgcccgg aaccggcgga ccggcaggtc gtggaaaagg gcctccgcct ccttgctctt
     6661 gaaggaccgg atcacggagg gagggtatta cgcatgacgt catgcgtcaa accatcccgg
     6721 gcgccgggag caaggcgcat cccgcgcgag cagcccatgg ccacgtgacc gggacggtgc
     6781 agaccctcgg gccccggggc cgtctacccc ctgcgaagcc gctccgcgtg cctttcgcac
     6841 tcggcccagc tctcctcgac ggactgcgcg agctgccagg ggaggaagcg ttcgccgagg
     6901 cgggatgcga cgggctgcgc ctcgcgggcg cggtcgtcgt ggccgaggcc gaggtcggcg
     6961 aggcggagcg tctcggggag catccagccg tcgccgtcgt gcgcgtcgca gtaggcggcg
     7021 atccccttct cgcggtcgcc cccgtgcgcg gcgatggtct gcttcaggtc gcggaaggcg
     7081 gcgagggaga ggacggtgtg ccgcagctcc gggtccttct ccttgtcgac gcgccagaag
     7141 cccttcgggt cgagcgtccg gcagaacgcc ttctcgcgca ggagcgccgc cgggtggtcg
     7201 cagtcgcgga ggatccacgc gaggtcgtcg aagtcgaggc cgtagagctc ggcgacgacg
     7261 gcgtcgagga gggcgcggag gcggaggcgt tcgtgggggg tgatggccca gagcgagcgc
     7321 caggggcgac caccgattgc tgcgccttca tcgctcttcg caactgcaag ccacgctgca
     7381 ctgaagacag ccgacgtg
//
LOCUS       DAOTEZ010000196         7372 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_7155_length_7372_cov_6.654503, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000196 DAOTEZ010000000
VERSION     DAOTEZ010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7372
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7155_length_7372_cov_6.654503"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="P5164_11245"
     CDS             <1..252
                     /locus_tag="P5164_11245"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRY44509.1"
                     /translation="VIYQRDSTGRFVYYYAHLSRYAPGLKVGDTVKRGQQIGEVGTTG
                     RTFGAHLHFAIFRDADAPNPWKGLVVNPYLVFSALVGGK"
     gene            356..2227
                     /locus_tag="P5164_11250"
     CDS             356..2227
                     /locus_tag="P5164_11250"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRY44510.1"
                     /translation="MSERVPFHHSAERFPEPLAEAVRLAGLADRPWADAPPEPDGGPA
                     LYLASSPVPEALAPLLHDGSPVVALSWGEPAELPIPVFSLPPHAPPAALASILSAAAE
                     VSAARAASASLSRRLSEAEERVSALNRIGIALSAERDLDRLLEKILTESRRFTSSEAG
                     SLYLLEEGPHGRRLRFKLAQNDAVKFAFSERTMPVDDASLAGYVAGHGESIVLEDAYA
                     VPAGAPYRLNAAFDEQTGFRTRSMAVVPMSDHTGALVGVVQLMNRRLPDGSPAPYPPD
                     LIPLLHSLATQAAVSVKANQLTASIRKLFEDFARAAIMAVELRDPTTAGHSNRVADLT
                     VSLARLVDRADDGPYSSVSFSREEMRELRTAALLHDFGKVSIPERVLVKAKKVDEEAI
                     LRIRDRFDFALESEDARDGRRLLERLAEAGIPPSPEDLRLLDAARWDRAQELEEMFEE
                     VRRANEPTILPEEAGGVLRRLLTRSYRDRRGVVRSLLQDDEFQLLSIRKGSLSVEERT
                     QIESHVSHTYRFLSSIPWTPDLARVPEIAHAHHEKLDGSGYPRGVRADEIPVASRVMT
                     ICDIYDALTASDRPYKKAVPREKALRILEDESRSGLLDAWLVSAFVGGKLWLPSGQP"
     gene            2224..2985
                     /locus_tag="P5164_11255"
     CDS             2224..2985
                     /locus_tag="P5164_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44511.1"
                     /translation="MRGAGRASLLFLLALSPVAPLPSAAAAEPGRALVPRGRWHLEAP
                     GPPEVRWSVLFRRSDAEDETRLLVETPGGRWSLLSKQPASGSDTREEVTDTALGETVS
                     RVLTHAPVVLFPECASVRPPDACLVLEGTRGRLAAPLSAFSKGEAAALRRKASAVVSP
                     DFLRRLRGLAPALPIADLAFYSEDFLALLDPALARPKGPPLVAPRRPGCTFDASFGWP
                     CTPDERRREEWLFRRPAVVTPERTGARDEGGRGPS"
     gene            complement(2933..4486)
                     /locus_tag="P5164_11260"
     CDS             complement(2933..4486)
                     /locus_tag="P5164_11260"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HRY44512.1"
                     /translation="MGGLFTERDLSTLARAGIDPAEAARQVELLTNPPAPAALVRPCT
                     RGDGIERLPEERHRDLLALFEEAEKEGRLSKLVPASGAATRMFQSLRKTLDEPETPFE
                     EWSARAARGDAAAGEVVRFVEGLEELPFAGELRAAAGEAVFSDPRARLAELLGVLLLP
                     SGLGLASRPKGLVPFHLEEEGARTPFEEHLVEAALTVRSASGRTPVHFTVADEARAGF
                     EALLERRRPDLERRLHARLDVSFSVQERATDTLAVALDGSPFRTAEGELLLRPGGHGA
                     LLGNLASLGADVVFVKNVDNVVPDSRKGPTLLWKKLLAGRLLEVERRVRNLVSLLDAG
                     APGAADEALSYLRETFGEATAEADTLPDDERRRWVRARLARPLRVCGVVRNEGEPGGG
                     PFWVRGRDGRLARQIVESAEVDLSDPAQRALWSSGTHFNPVDLVLALRDGEGRPWDLP
                     RFVDEAAVFVSKKSHEGVPLLALERPGLWNGAMAFWNTLFVEVPIETFAPVKTVLDLL
                     RPEHQSAPG"
     gene            complement(4489..5385)
                     /gene="rfbA"
                     /locus_tag="P5164_11265"
     CDS             complement(4489..5385)
                     /gene="rfbA"
                     /locus_tag="P5164_11265"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HRY44513.1"
                     /translation="MKGIILAGGSGTRLHPVTRGVSKQLVPVYDKPMIYYPLSTLMLA
                     GIREVLVITTPHEQEGFRRLLGDGAWLGLKIEYAAQPSPDGLAQAFLIGREFVGEGRV
                     ALALGDNIFYGHGFPELLQAAARRESGATVFAYRVRDPERYGVVEFGADGRAVSLEEK
                     PSKPRSSYAVTGLYFYDNRVLDVAANLKPSPRGELEITDVNRVFLEWGELNVEVLGRG
                     VAWLDTGTHASLLQASNFMQAIEERQGLKVACLEEVAYRMGYIGTDEVREIAVTMRNN
                     EYGQYLLRLLEEEAEAGARTAR"
     gene            5476..>7372
                     /locus_tag="P5164_11270"
     CDS             5476..>7372
                     /locus_tag="P5164_11270"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRY44514.1"
                     /translation="MLRLLRTPLLLAALLVALPGAAADTHPFSIHDMLAMDRISDVQL
                     SPDGTQAAFVLRTTDLAANRGRTDLWLLDVRSKGVRRLTTHEAADSSPRWASDGKSLY
                     FLSTRGGSQQVWRIPLAGGEAERVSSEPLDVDNLEVAPGGKLLVISMEVFPGKSPAET
                     KAALDAKESVKASGMLYDRLFVRHWDTWKDGRRNHVFALPLGADGKAAGEAKDLMPAM
                     DADAPPKPFGGLDDAAISPDGKTLVFTCKDVGREEAWSTNYDLWEVPTDGSAAPKRIT
                     TNPAWDSTPRFSPDGKTLAYAAMSRAGYEADRFEVVFRDLATGKDRSFTLRAGPGKND
                     DRSPASLTWSADGKSLLATADHLGQKALFAIDAATGRAKILLADGSVDSASPLGKDRI
                     LFARHSLLGPTEIYAMPVAGGAYEKLTSINDAKVAAARFGKPEQFTFTGAKGDTVYGW
                     IVYPVDFDPAKKYPVAYLIHGGPQGSFGNDFHYRWNPQAYAGAGYATVMIDFHASTGY
                     GQAFTDAVNGDWGGAPYEDLMKGLDFVLGKYAFLDGNRVGALGASYGGYMINWIHGQT
                     DRFRCLVVHDGNLDERMAYYDTEELWFPEWENGGTPWTAKSFGHHNPIDYVKNWKTPT
                     LVIHGQKD"
BASE COUNT         1029 a         2831 c         2471 g         1041 t
ORIGIN      
        1 gtcatctacc agcgggactc gaccggccga ttcgtctact actacgccca cctctcccgc
       61 tacgcgcccg gcctgaaggt cggcgacacg gtgaagcgcg gccagcagat cggcgaggtc
      121 ggcacgacgg gtcggacgtt cggcgcgcac ctccacttcg ccatcttccg cgacgccgac
      181 gccccgaacc cctggaaggg gctcgtggtg aacccgtacc tcgtcttctc ggcgctcgtc
      241 ggcgggaagt gacccgcggc ggcgtgccgc ccgcccggta cgcgccgctc ccgtctccac
      301 gttccggtac gcctctccgt tgaccctccg gggcacgggc gcacagaatc cggtcatgag
      361 cgagcgcgtc ccgttccatc actcggcgga gcgctttccg gagccgctcg ccgaagccgt
      421 ccgcctcgcc gggctcgccg accggccctg ggcggacgcc ccgcccgagc ccgacggagg
      481 cccggccctc tacctcgctt cctcgcccgt cccggaggcg ctcgcccctc tcctgcacga
      541 cggctccccc gtcgtcgccc tgtcctgggg cgagccggcg gagctcccga tccccgtctt
      601 ctccctgccg ccccacgctc cgccggcggc gctcgcgtcg atcctctcgg ccgcggccga
      661 ggtctcggcg gcgcgggcgg cctcggcgtc gctttcgcga aggctctccg aggccgaaga
      721 acgcgtcagc gccctgaacc ggatcgggat cgcgctttcg gccgagcggg acctcgaccg
      781 cctcctcgag aagatcctca cggagtcgcg gcgcttcacc tcgtcggagg cggggagcct
      841 ctacctcctc gaggaagggc cccacggccg acggctccgc ttcaagctcg cccagaacga
      901 cgcggtgaag ttcgcgttct ccgagcggac gatgccggtc gacgacgcga gcctcgccgg
      961 ctacgtcgcg ggccacggcg agtcgatcgt cctcgaggac gcctacgccg tgccggccgg
     1021 tgccccgtat cgcctcaacg cggccttcga cgagcagacc ggcttccgga cccgctccat
     1081 ggccgtcgtc ccgatgtccg accacacggg cgcgctcgtc ggagtcgtcc agctgatgaa
     1141 ccggcgcctc ccggacggca gcccggcccc gtaccctccg gacctgatac cgctcctgca
     1201 ctctctcgcg acgcaggcgg ccgtgtcggt caaggcgaac cagctgaccg cttccatccg
     1261 gaagctcttc gaggacttcg cgcgcgccgc gatcatggcc gtcgagctgc gcgacccgac
     1321 gaccgcgggt cacagcaacc gcgtggccga cctgaccgtc tccctcgccc gcctcgtcga
     1381 ccgcgcggac gacggcccct actcctccgt ctcgttctcg cgcgaggaga tgcgcgagct
     1441 ccggacggcg gcgctcctcc acgacttcgg gaaggtctcg atcccggagc gggtcctcgt
     1501 caaggcgaag aaggtcgacg aggaggcgat cctccggatc cgcgaccggt tcgacttcgc
     1561 gctcgagtcc gaggacgccc gcgacggccg gagactcctc gaacggctcg ccgaggccgg
     1621 catcccccct tcgccggagg acctccgcct tctcgacgcc gcgcgctggg accgggcgca
     1681 ggagctcgag gagatgttcg aggaggtccg ccgcgcgaac gagccgacga tcctccccga
     1741 ggaggcgggc ggcgtcctcc ggcggctcct gacgcgctcc tatcgcgacc gcaggggcgt
     1801 cgtgaggtcg ctgctccagg acgacgagtt ccagctcctc tcgatccgga agggaagcct
     1861 ctcggtggag gagcggaccc agatcgagag ccacgtctcc catacctacc ggttcctctc
     1921 ctcgattccg tggaccccgg acctcgcccg ggtcccggag atcgcgcacg cccaccacga
     1981 gaagctcgac ggctccgggt acccgcgcgg cgtgcgggcg gacgagatcc cggtcgcgtc
     2041 gcgcgtcatg acgatctgcg acatctacga cgcgctgacg gcctcggacc ggccctacaa
     2101 gaaggccgtg ccgcgggaga aggcgctccg gatcctcgag gacgagagcc gctccggcct
     2161 cctcgacgcc tggctggtct cggccttcgt cggcgggaag ctctggctcc cctcggggca
     2221 gccttgaggg gcgcggggcg cgcctctctc ctcttcctcc tcgcgctctc tccggtggcg
     2281 ccgcttccgt cggccgcggc cgccgagccc gggcgcgccc tcgtcccgcg cgggcgctgg
     2341 cacctcgagg cgccagggcc tccggaggtc cgctggtcgg tcctcttccg ccgcagcgac
     2401 gcggaggacg agacccgcct cctcgtcgaa accccgggcg ggcgctggtc gctcctctcg
     2461 aagcagcccg cctccggctc cgacacacgc gaggaggtga ccgacaccgc gctcggcgag
     2521 acggtctcgc gcgtcctcac gcatgcgccc gtcgtcctct tcccggagtg cgcgtcggta
     2581 cgccctcctg acgcctgcct cgtcctcgag gggacgcgcg gccgcctggc cgcgccgctc
     2641 tcggcgttct cgaaaggaga ggccgccgcc ctgaggcgga aggcctccgc cgtcgtctct
     2701 cccgacttcc tccgacggct ccggggactc gcgcccgccc tcccgatcgc cgacctcgcg
     2761 ttctactcgg aggacttcct cgcgctgctc gacccggcgc tcgcgaggcc gaaggggccg
     2821 ccgctcgtcg ccccccgccg tccgggctgc acgttcgacg cgagcttcgg gtggccctgc
     2881 acccccgacg agagacggcg cgaggagtgg ctcttccgcc gccccgccgt cgtcaccccg
     2941 gagcggactg gtgctcggga cgaaggaggt cgaggaccgt cttgacgggc gcgaaggtct
     3001 cgatcggcac ctcgacgaag agcgtgttcc agaaggccat cgcgccgttc cagaggcccg
     3061 gccgctcgag cgccaggagc gggacaccct cgtgcgactt cttcgagacg aagaccgccg
     3121 cctcgtcgac gaatctcggg aggtcccacg ggcgtccctc gccgtcgcga agcgcgagga
     3181 cgagatcgac gggattgaag tgcgtcccgg agctccagag cgccctctgc gccgggtcgg
     3241 agaggtcgac ttcggcgctc tcgacgatct ggcgggcgag cctcccgtcg cggcctctca
     3301 cccagaacgg cccgccgccg ggctccccct cgttcctcac gacgccgcag acgcgcaggg
     3361 ggcgcgcgag acgggcgcgc acccaccggc ggcgctcgtc gtccgggagc gtgtcggcct
     3421 cggccgtcgc ctccccgaac gtctcgcgga ggtaggagag cgcctcgtcg gccgctccag
     3481 gtgctcccgc gtcgaggagc gagacgaggt tccggaccct ccgctccacc tcgaggagcc
     3541 tccccgcgag cagcttcttc cagaggagcg tcgggccctt cctcgagtcg gggacgacgt
     3601 tgtcgacgtt cttcacgaag acgacgtccg ctccgaggga ggcgaggttc ccgagcaggg
     3661 ctccgtgccc gcccgggcgg aggagcagct ctccctccgc cgtccgaaag ggactcccgt
     3721 cgagcgcgac ggcgagcgtg tcggtcgcgc gttcctggac cgagaaagag acgtcgaggc
     3781 gggcgtggag ccggcgctcg aggtccggcc ggcgtctctc gaggagcgcc tcgaagccgg
     3841 cccgcgcctc gtcggcgacg gtgaagtgga ccggagtccg gcccgaagcc gagcggacgg
     3901 tcagcgccgc ctcgacgagg tgctcctcga acggcgttcg cgccccctct tcctcgaggt
     3961 ggaacgggac gagccccttc ggacgggagg ccaggccgag ccccgacggc aggaggagca
     4021 ccccgagcag ctcggcgagg cgcgcacgcg gatcggagaa gaccgcctcc cccgcggccg
     4081 cgcgcagctc cccggcgaac ggcagctcct cgagcccctc cacgaaccgg acgacctccc
     4141 cggccgccgc gtcgccccgc gccgcgcgcg cgctccactc ctcgaacggg gtctccggct
     4201 cgtcgagcgt cttccggagc gactggaaca tccgtgtcgc cgcgcccgag gccgggacga
     4261 gcttcgagag gcgcccctcc ttctcggcct cctcgaagag ggcgagaagg tcgcggtggc
     4321 gctcctcggg gagccgctcg atcccgtcgc ctcgcgtgca ggggcggacg agcgcggccg
     4381 gggcgggagg gttcgtcagg agctcgacct gccgggccgc ttcggccgga tcgattccgg
     4441 cccgggccag cgtcgagaga tcccgctccg tgaagaggcc gcccacggtc acctcgccgt
     4501 ccgcgcgccc gcctccgcct cctcctccag gagccggagc aggtactgcc cgtactcgtt
     4561 gttcctcatc gtgacggcga tctcgcggac ctcgtccgtc ccgatgtagc ccatccggta
     4621 ggcgacctcc tcgaggcacg ccaccttcag cccctgccgc tcctcgatcg cctgcatgaa
     4681 gttcgaggcc tgcaggagcg aagcgtgcgt cccggtgtcg agccacgcga ccccgcggcc
     4741 gaggacctcg acgttcagct cgccccattc gaggaagacg cggttcacgt cggtgatctc
     4801 gagctccccg cgcggcgaag gcttcaggtt cgccgccacg tcgaggaccc ggttgtcgta
     4861 gaagtagagc cccgtcaccg cgtaggagga ccggggcttc gacggcttct cctcgaggct
     4921 caccgcacgc ccgtcggccc cgaactccac gacgccgtag cgctcgggat cgcggacccg
     4981 gtaggcgaag accgtcgcgc cgctctccct ccgcgcggcc gcctggagga gctccgggaa
     5041 gccgtgcccg tagaagatgt tgtcgccgag cgccagggcc acccgtccct cgccgacgaa
     5101 ctcgcgtccg atcaggaacg cctgagccag tccgtccggg ctcggctggg ccgcgtactc
     5161 gatcttcagg ccgagccagg cgccgtcccc gaggagccgc cggaacccct cctgctcgtg
     5221 cggcgtcgtg atgacgagga cctcccggat ccccgccagc atgagcgtcg agagcgggta
     5281 gtagatcatc ggcttgtcgt agacggggac gagctgcttg ctgacgccgc gagtcaccgg
     5341 gtggagacgc gtgccggatc ccccggcgag gatgattccc ttcacttcgg actcctcgct
     5401 ccgatcttag ccggggaggc ccggcccggg cgcttccgcg cccgtccggg ccccgtctcg
     5461 ggctacgatc cggccatgct ccgcctcctg cgcaccccgc tcctcctcgc ggcgctcctc
     5521 gtcgccctcc cgggcgcggc cgccgacacc caccccttct ccatccacga catgctcgcg
     5581 atggaccgga tctccgacgt ccagctctcg cccgacggga cgcaggcggc gttcgtcctc
     5641 cggacgaccg acctcgccgc gaaccgcggc cggaccgacc tctggctcct cgacgtgagg
     5701 tcgaagggcg tccgccgcct gacgacgcac gaggcggccg actcgagccc ccgctgggcg
     5761 tcggacggga agagcctcta cttcctctcg acgcgcggcg gctcccagca ggtctggcgc
     5821 attcccctcg cgggaggcga agcggagagg gtctccagcg agccgctcga cgtcgacaac
     5881 ctggaggtcg cgccgggcgg gaagctcctc gtgatctcga tggaggtctt ccccgggaag
     5941 agccccgccg agacgaaggc cgcgctcgac gcgaaggagt ccgtcaaggc gagcgggatg
     6001 ctctatgacc gcctcttcgt gcggcactgg gacacctgga aggacgggcg acggaaccac
     6061 gtcttcgcgc tcccgctcgg cgccgacggg aaggcggccg gtgaggcgaa ggacctgatg
     6121 ccggcgatgg acgccgacgc gccgccgaag ccgttcggcg gcctcgacga cgccgcgatc
     6181 agccccgacg ggaagacgct cgtcttcacc tgcaaggacg tcgggcgcga agaggcctgg
     6241 tcgacgaact acgacctctg ggaggtcccg accgacggtt cggccgcgcc gaagcggatc
     6301 acgacgaacc cggcctggga ttcgactccg cgcttctctc cggacgggaa gacgctggcg
     6361 tacgcggcga tgagccgcgc gggctacgag gccgatcgct tcgaggtcgt tttccgcgac
     6421 ctcgcgaccg ggaaggaccg ttcgttcacc ctccgcgccg gtcccgggaa gaacgacgac
     6481 cgctcccccg cgagcctgac ctggtcggcg gacgggaagt cgctcctcgc caccgccgac
     6541 cacctcggcc agaaggccct tttcgcgatc gacgcggcga ccggcagggc gaagatcctc
     6601 ctcgccgacg gctccgtcga ctccgcctcc ccgctcggca aggaccggat cctcttcgcg
     6661 cgtcactccc tcctcgggcc gaccgagatc tacgcgatgc cggtcgcagg cggcgcgtac
     6721 gagaagctga cctcgatcaa cgacgcgaag gtcgccgcgg cccgcttcgg caagcccgag
     6781 cagttcacgt tcacgggcgc gaagggggac accgtctacg gctggatcgt ctacccggtc
     6841 gacttcgacc cggcgaagaa gtaccccgtc gcgtacctca tccacggcgg cccgcagggc
     6901 tccttcggga acgacttcca ctaccgctgg aaccctcagg cctacgccgg cgccggctac
     6961 gcgaccgtga tgatcgactt ccacgcctcg accggctacg gccaggcctt caccgacgcc
     7021 gtgaacggcg actggggcgg cgcgccctac gaggacctga tgaagggcct cgacttcgtc
     7081 ctcggcaagt acgccttcct ggacgggaac cgcgtcgggg cgctcggcgc gtcgtacggc
     7141 ggctacatga tcaactggat ccacggccag accgaccgct tcaggtgcct cgtcgtccac
     7201 gacgggaacc tcgacgagcg gatggcctac tacgacaccg aggagctctg gttccccgag
     7261 tgggagaacg gcgggacgcc ctggacggcg aagagcttcg ggcaccacaa cccgatcgac
     7321 tacgtgaaga actggaagac cccgacgctc gtcatccacg gccagaagga ct
//
LOCUS       DAOTEZ010000197         7371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_7156_length_7371_cov_6.337343, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000197 DAOTEZ010000000
VERSION     DAOTEZ010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7371
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7156_length_7371_cov_6.337343"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..802)
                     /locus_tag="P5164_11275"
     CDS             complement(170..802)
                     /locus_tag="P5164_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRY44515.1"
                     /translation="MRIDLPSGRRLGAAALAVAALLAAGCRQGMYDQPKEKPLGRSDF
                     FRDGLASRVPPAGTVARGWLREDRVLHTGIGPDGKFVSELPAAVPFDRALLDRGHQRY
                     DVFCSPCHGRQGNGEGMIVQRGFKQPASFHVDRLRAQPLGYYFDVMTNGFNEMSSYAS
                     QVPVKDRWAIAAYVRTLQLSRNAPASELSPADLEALEKSAAPAAAPEVHP"
     gene            complement(792..1343)
                     /locus_tag="P5164_11280"
     CDS             complement(792..1343)
                     /locus_tag="P5164_11280"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HRY44516.1"
                     /translation="MHGTHPEPSLHGVLAEFRTPDELLAAIESARAAGYTKLDAYTPY
                     PIEKVLDALDLHHSKVPLLTLGAAIFGAVAVFSFAYWASAIDYPLNIGGRPLNSWPAF
                     IPATFEGAVFHGGLAAALGMLVLNGLPRPHHPVFEVKRFVEHASKDGYFLLVEAEDPQ
                     FDVSAVRRLLEGLGPVEVNDLEN"
     gene            complement(1348..2748)
                     /gene="nrfD"
                     /locus_tag="P5164_11285"
     CDS             complement(1348..2748)
                     /gene="nrfD"
                     /locus_tag="P5164_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HRY44517.1"
                     /translation="MSIRFDDVRGADPIAQPPVLAPGHTPASVTERISEAVTTKLTKT
                     PKWWLGGFAVGFALLNVFLFAVTNLFMTGIGVWGNNIPVGWGWDIICFVWWIGIGHAG
                     TLISAILLLLRQSWRNSINRFAEAMTVFAVACAGMYPILHLGRPWLPHWLLPLPGTLG
                     MWPNFRSPLIWDVFAVSTYATISIVFWYVGMLPDLATLRDHAKARLARFAYGMAALGW
                     RNSSRHWANYEVASLLLAGLSTPLVLSVHSIVSFDFSVGIVPGWHTTVFPPYFVAGAV
                     FAGFAMVLTLVLPLRPLFKLHDFITERHLQNMAKVMLTTGLIVAYGYVLEIWAAFYSG
                     NEFEIFMVLNRFTGPYATQYWLLILLNIAIPQLLWLKGIRSNVWALWAISMCINVGMW
                     LERFVIIVVSLHRDYVPSNWAMYSGTKFDVMTFVGTMGLFLTLLLLFIRFLPALNMFE
                     MRILTPEATKAHGEEK"
     gene            complement(2751..5798)
                     /locus_tag="P5164_11290"
     CDS             complement(2751..5798)
                     /locus_tag="P5164_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HRY44518.1"
                     /translation="MKSLASPGPVDDAPARFPIDLAAIRGRLDRRQGPAFWRGLEELA
                     DDAGFREFLATEYPRHASDWDESLDRGFSRRRFLELGMASMALAGMTACTRQPVEKIV
                     PYVKQPEEIVPGVPLFFATAATIGGFAQPLLAESHEGRPTKVEGNPEHPASLGGATAV
                     AQASVLGLYDPDRLKVPTKLGILDTWPRLVTELQAAAKAQKQVEGAGLRVLTQTVTSP
                     TLGAQLAALLGAYPKAKWHQWEPAGRDAVRAGARLAFGRAVETRYDLGAADVVVALDS
                     DLLASGPDSVRNAKAFSARRRLTDGRTETSRFYAVEVSPTPTGTLADHRLPLRASLLP
                     AFAQALAAALGLPGFAAPAGLGEQAAKLVAAAARDLRGSAGRSVAVAGEFAPPEVHAA
                     AHAINEALGNVGKTVFLSEPVEAAPIDQLASLHELVEDVKAGSVELLLILGGNPVYDA
                     PSDLAFGEALLKAGLTVALASHPDETTRYCHWAVPMAHDLEAWSDARSLDGTATLVQP
                     LIEPLYEGKSAHELLSVLLDEVPRKGYDVVKDSWKAARPAEADFEAFWRKALHDGVVP
                     GTAAPSVAAPVRLAEVAAALAAASAAKPAGLEAAFRPDPSLLDGRYANNGWLQELPKP
                     LSKLTWDNAAVVSPATAQELGVTNEDVVKVEAGGRSVELPVLIQPGQAAGVVTLHLGF
                     GRKHAGRVGNGVGGDVTPLRTTAAPWLVPAVTVTKTGGRHPLAHTQQHFRMEGRDIVR
                     TMTLPEYRKDPAKAKGHGLPPKEASLFQPGWEYKENAWGLSVDLGSCFGCNACVVACQ
                     SENNVPVVGKEQVAKNREMHWIRVDQYYEGSDLESPAVHHQPLMCQHCEMAPCEVVCP
                     VAATVHGAEGLNEMAYNRCVGTRYCSNNCPYKVRRFNFLEYNGQREEEPVLKMLANPD
                     VTVRSRGVMEKCTYCIQRINRARIAAEREGRAIRDGEIKTACQQTCPADAIVFGNIND
                     PAAGVSKLRSEPRTYWLLEELNTRPRTTYLARVTNPSPDVEGA"
     gene            complement(5819..6475)
                     /locus_tag="P5164_11295"
     CDS             complement(5819..6475)
                     /locus_tag="P5164_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRY44519.1"
                     /translation="MAQLFPKSANSLAPASLAALVLVAGGAVGSLLALDYAGFNQRRG
                     ETVEQKVPFSHEHHVAGLGIDCRYCHTSVETSAVAGIPPTSTCYNCHKTVWNEAPMLE
                     PVRSSAKTGQPLEWTKVHDLPDFAYFNHSIHVAKGIGCATCHGPVQEMRLMRSAQPLQ
                     MRWCLDCHRNPERYIRPKEEIYNMTWKAKDQETLGRELVKAYGVRPPAQITSCGTCHR
                     "
     gene            complement(6848..>7371)
                     /locus_tag="P5164_11300"
     CDS             complement(6848..>7371)
                     /locus_tag="P5164_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate carboxyltransferase"
                     /protein_id="HRY44520.1"
                     /translation="EGPTRLEDAVTFTKRFLEIGADDIEHADHDGSAPPDRVYAYYAM
                     VLDEIPDPGKHVAHFHVTRGWGLANVLAALQAGITRYESTLGGIGGQPANFFDGAPVS
                     GTGRYYYQDPNNVGLVCTEDLAVMLDEMGIETGLDVDRVLATGRMVERIVGRRLRSET
                     VKSGRIPKKPTGF"
BASE COUNT         1074 a         2350 c         2872 g         1075 t
ORIGIN      
        1 ccggcggcga ggccgagcat ccacatgtag ccggtgaggt aggagcggaa gagctgatct
       61 cggtcggtgg cgtacccggc cccgaggagc gcgaggccga cgagtccggc gatgagacca
      121 atccggccga gcgtctcgac gccctcgggg agggggttcg agccctggtt cacgggtgga
      181 cctccggcgc ggccgcgggg gcggcggact tctcgagcgc ctcgaggtcc gcgggggaga
      241 gctcggaggc gggggcgttg cgggagagct ggagggtgcg gacataggcc gcgatcgccc
      301 agcggtcctt caccgggacc tggctcgcgt agctcgacat ctcgttgaag ccgtttgtca
      361 tcacgtcgaa gtagtagccg agcggctggg cgcggaggcg gtcgacgtgg aaggacgccg
      421 gctgcttgaa gccgcgctgg acgatcatcc cctcgccgtt cccctgccgg ccgtggcagg
      481 gggagcagaa gacgtcgtag cgctggtggc cccggtcgag gagcgcgcgg tcgaacggca
      541 cggcggccgg gagctcggag acgaacttcc cgtcggggcc gatcccggtg tggaggaccc
      601 ggtcctcgcg gagccagccg cgggcgacgg tccccgcggg cgggacgcgg gaggccaggc
      661 cgtcgcggaa gaagtcgctc cggccgagcg gcttctcctt cggctggtcg tacatcccct
      721 gccggcagcc cgcggcgagg agcgccgcga cggcgagtgc ggcggctccg aggcggcggc
      781 cggacggaag gtcaattctc aaggtcgttg acctccacgg gcccgaggcc ctccagcagc
      841 cgccggacgg cggaaacgtc gaactgcggg tcctcggcct cgacgaggag gaaatagccg
      901 tccttcgagg cgtgctcgac gaacctcttc acctcgaaga cggggtggtg cggccgcggc
      961 aggccgttca ggacgagcat ccccagcgcg gccgcgagcc ctccgtggaa gaccgcccct
     1021 tcgaacgtcg cggggatgaa ggcgggccag gagttgaggg gccgcccgcc gatgttgagc
     1081 gggtagtcga tcgcgctcgc ccagtaggcg aaggagaaga ccgccacggc gccgaagatc
     1141 gccgccccga gcgtcaggag cggcaccttc gagtggtgga ggtccagggc gtcgaggacc
     1201 ttctcgatcg gatagggcgt gtaggcgtcg agcttcgtgt agccggcggc cctcgcggac
     1261 tcgatcgccg cgaggagctc gtcgggcgtg cggaactcgg cgaggacgcc gtggagggac
     1321 ggctcggggt gcgtgccgtg catgacgcta cttctcctcg ccgtgggcct tcgtggcctc
     1381 gggcgtgagg atgcgcatct cgaacatgtt cagcgcgggg aggaagcgga tgaagaggag
     1441 gaggagcgtc aggaagagac ccatcgtccc gacgaacgtc atcacgtcga acttcgtccc
     1501 cgagtacatc gcccagttcg aggggacgta gtcgcggtgg aggctgacga cgatgatgac
     1561 gaaccgctcg agccacatcc cgacgttgat gcacatcgag atcgcccaga gcgcccagac
     1621 gttcgagcgg atccccttga gccagaggag ctgcgggatc gcgatgttga ggaggatgag
     1681 gagccagtac tgcgtggcgt acggcccggt gaagcggttc aggaccatga agatctcgaa
     1741 ctcgttcccg ctgtagaagg ccgcccagat ctcgaggacg tagccgtagg cgacgatgag
     1801 gccggtcgtc agcatcacct tcgccatgtt ctgcaggtgc cgctcggtga tgaagtcgtg
     1861 gagcttgaag agcggccgga gcggcaggac gagggtgagg accatcgcga agccggcgaa
     1921 gacggccccg gcgacgaagt aggggggaaa gaccgtcgtg tgccagccgg ggacgatgcc
     1981 gaccgagaag tcgaacgaga cgatggagtg gaccgaaagg acgagcggcg tcgagaggcc
     2041 cgcgaggagg agcgaggcga cctcgtagtt cgcccagtgg cgcgaggagt tgcgccagcc
     2101 gagggccgcc atgccgtagg cgaagcgggc gagcctcgcc ttcgcgtggt cgcggagcgt
     2161 cgcgaggtcg gggagcatcc cgacgtacca gaagacgatc gagatcgtcg cgtaggtcga
     2221 gacggcgaag acgtcccaga tgagggggct ccggaagttc ggccacatgc cgagagtccc
     2281 cgggagcggg aggagccagt gggggagcca ggggcggccg aggtggagga tcgggtacat
     2341 gccggcgcag gcgacggcga agaccgtcat cgcctcggcg aagcggttga tcgagttgcg
     2401 ccagctctgc cggaggagga ggaggatcgc ggagatgagc gtccccgcgt gcccgatgcc
     2461 gatccaccag acgaagcaga tgatgtccca gccccagccg accgggatgt tgttccccca
     2521 gacgccgatg ccggtcatga agaggttcgt gacggcgaag aggaagacgt tcaggagggc
     2581 gaagccgacg gcgaacccgc cgagccacca tttcggcgtc ttcgtcagct tcgtggtgac
     2641 ggcctcgctg atccgctcgg tgacggaggc cggcgtgtga cccggggcga ggacgggagg
     2701 ctgcgcgatc gggtcggcgc cgcggacgtc gtcgaagcgg atcgacatga tcaggcgccc
     2761 tccacgtcgg ggctcgggtt cgtgacccgg gcgaggtagg tcgtccgcgg ccgggtgttc
     2821 agctcctcga ggagccagta ggtccgcggc tcgctccgga gcttcgagac gcccgcggcg
     2881 gggtcgttga tgttcccgaa gacgatcgcg tcggccgggc aggtctgctg gcaggccgtc
     2941 ttgatctcgc cgtcgcgtat ggcgcgcccc tcgcgctcgg cggcgatgcg cgcgcggttg
     3001 atccgctgga tgcagtaggt gcacttctcc atgacgcccc gcgagcggac ggtgacgtcc
     3061 gggttcgcga gcatcttcag gaccggctcc tcctccctct gtccgttgta ctcgaggaag
     3121 ttgaagcgcc ggaccttgta ggggcagttg ttcgagcagt agcgcgtgcc gacgcagcgg
     3181 ttgtaggcca tctcgttgag cccctccgcg ccgtggaccg tcgcggcgac ggggcagacg
     3241 acctcgcacg gcgccatctc gcagtgctgg cacatgaggg gctggtggtg gacggccggg
     3301 ctctcgaggt cgcttccctc gtagtactgg tcgacgcgga tccagtgcat ctcgcggttc
     3361 ttcgcgacct gctccttgcc gacgacgggg acgttgttct ccgactggca ggcgacgacg
     3421 caggcgttgc agccgaagca cgagccgagg tcgacggaga gcccccaggc gttctccttg
     3481 tactcccagc cgggctggaa gagcgacgct tccttcggcg ggagtccgtg ccccttcgcc
     3541 ttcgccgggt ctttccggta ctcggggagc gtcatcgtcc ggacgatgtc gcgcccctcc
     3601 atccggaagt gctgctgcgt gtgggcgagg ggatggcgcc cgccggtctt cgtcaccgtg
     3661 accgccggga cgagccaggg ggcggccgtg gtccggaggg gggtgacgtc gccgccgacg
     3721 ccgttgccga cgcggccggc gtgcttccgg ccgaagccga ggtggagcgt gacgacgccc
     3781 gcagcctggc ccggctggat caggacgggg agctcgacgg agcgcccgcc cgcctcgacc
     3841 ttcacgacgt cctcgttcgt gacgccgagc tcctgggccg tcgccggcga gacgaccgcg
     3901 gcgttgtccc acgtcagctt gctgagcggc ttcgggagct cctggagcca gccgttgttc
     3961 gcgtagcggc cgtcgaggag ggaggggtcg gggcggaacg ccgcctcgag ccctgcgggc
     4021 ttcgcggcgg aggcggcggc gagcgcggca gccacctcgg cgaggcggac cggcgccgcg
     4081 acggaggggg cggccgtccc ggggacgacg ccgtcgtgca gcgccttccg ccagaacgcc
     4141 tcgaagtcgg cctcggcggg gcgcgccgcc ttccaggagt ccttcacgac gtcgtagccc
     4201 ttccggggga cctcgtcgag gaggacggag aggagctcgt gcgccgactt cccctcgtag
     4261 agcggctcga tcagcggctg gacgagcgtc gcggtgccgt cgagggagcg agcgtcgctc
     4321 cacgcctcga ggtcgtgggc catcgggacg gcccagtggc agtagcgggt cgtctcgtcg
     4381 gggtggctgg cgagggcgac cgtcaggccc gccttcagga gcgcctcgcc gaacgcgagg
     4441 tcggagggcg cgtcgtagac cgggttcccg ccgaggatca ggaggagctc cacggagccc
     4501 gccttcacgt cttccacgag ctcgtgaagc gaggcgagct ggtcgatcgg ggcggcctcg
     4561 accggctccg acaggaagac ggtcttcccg acgttgccga gcgcctcgtt gatcgcgtgc
     4621 gccgcggcgt ggacctcggg cggggcgaac tcgcccgcga cggcgacgga gcggcccgcg
     4681 gagccgcgaa gatcgcgggc cgcggctgcg acgagcttcg ccgcctgctc gccgaggccg
     4741 gccggcgcgg cgaagccggg gaggccgagg gcggcggcga gggcctgcgc gaaggcaggg
     4801 aggaggctcg cgcggagcgg gaggcggtgg tcggcgagcg tgccggtcgg ggtcggggag
     4861 acctccacgg cgtagaagcg cgacgtctcg gtccgtccgt ccgtgaggcg gcggcgggcg
     4921 gagaaggcct tcgcgtttcg gaccgagtcg ggcccggagg cgaggagatc ggagtcgagc
     4981 gcgacgacga cgtcggcggc gccgaggtcg tagcgggtct cgacggcgcg cccgaacgcg
     5041 aggcgcgcac ccgcccggac ggcgtcgcga ccggccggct cccactggtg ccacttcgcc
     5101 ttcgggtagg cgccgaggag cgccgcgagc tgcgcgccga gggtcgggga ggtgacggtc
     5161 tgcgtcagga cgcgcagccc cgccccctcc acctgcttct gggccttcgc ggccgcctgg
     5221 agctcggtga cgaggcgcgg ccaggtgtcg aggatgccga gcttcgtcgg gaccttcagg
     5281 cggtcgggat cgtagaggcc gaggacggag gcctgcgcaa cggcggtggc gccgccgagg
     5341 ctcgcggggt gctcggggtt cccctcgacc ttcgtcgggc gcccctcgtg gctctcggcg
     5401 aggagcggct gggcgaagcc gccgatcgtc gcggccgtcg cgaagaagag cggcacgccg
     5461 gggacgatct cctcgggctg cttcacgtac gggacgatct tctcgacggg ctgccgggtg
     5521 caggccgtca tcccggcgag ggccatcgag gccatcccga gctcgaggaa ccgccgccgc
     5581 gagaagccgc ggtcgaggct ctcgtcccag tcggaagcgt ggcgggggta ctccgtcgcc
     5641 aggaactcgc ggaagcccgc gtcgtcggcg agctcctcga ggccgcgcca gaaggcgggt
     5701 ccctggcgcc ggtcgaggcg gccgcggatc gcggcgaggt cgatcgggaa ccgggcgggc
     5761 gcgtcatcca cgggcccggg gctcgcaagg ctcttcaagt ctctctcctc gccccggctc
     5821 atcggtggca cgtcccgcag gaggtgatct gggcgggcgg gcggacgccg taggccttca
     5881 cgagctcgcg gccgagcgtc tcctggtcct tcgccttcca ggtcatgttg tagatctctt
     5941 ccttcggccg gatgtacctc tcggggttcc ggtgacagtc gaggcaccag cgcatctgga
     6001 gcggctgggc cgagcgcatg agccgcatct cctggaccgg tccgtgacac gtcgcgcagc
     6061 cgatcccctt cgcgacgtgg atcgagtggt tgaagtaggc gaagtcgggg aggtcgtgga
     6121 ccttcgtcca ctcgagcggc tggcccgtct tcgcgctcga gcggaccggc tcgagcatcg
     6181 gcgcctcgtt ccagacggtc ttgtggcagt tgtagcaggt ggaggtgggg gggattccgg
     6241 cgacggccga ggtctctacg gaggtgtggc aataccggca gtcgatgccg aggccggcga
     6301 cgtggtgctc gtggctgaag gggaccttct gctcgaccgt ctcgccgcgc cgctggttga
     6361 agccggcgta gtcgagcgcc aggagggagc cgacggcgcc tcccgccacg aggaccagcg
     6421 cggcgaggga ggcgggcgcg agggagttcg cgctcttcgg aaagagctgg gccatctgcg
     6481 gttacgcctt tctcggagaa cggcggggaa tatcgacccc tcccggggcc gtttcgggtt
     6541 cgggcggatt ccccggagct gtcgcggacg ggcggaggtg gtgccgggac ctccctgtac
     6601 agtgcaaatc cctctcctcg cggtgggtcg ctgttgagcg acgatctcaa aaaaccgtgg
     6661 aaggtaccac acgacggcga gcgcgaacag gccccgatcc ggtaccgatt gaagaccggg
     6721 tggacgacgg gccggaaggc ggagcatcgc cttcgcaagc gccgcgggcg cggcgaagcg
     6781 tcccgccccg cgccggacgt ccggccagct ggtccggtct ttcgcgccgg tcccgggggg
     6841 ccgctcgtca gaacccggtc ggcttcttcg ggatccggcc cgacttgacg gtctcgctcc
     6901 ggaggcgccg gccaacgatc cgctcgacca tccgccccgt ggcgaggacg cggtcgacgt
     6961 cgaggccggt ctcgatcccc atctcgtcga gcatcacggc gaggtcctcg gtgcagacga
     7021 ggccgacgtt gttcgggtcc tggtagtagt agcggccggt gccggagacg ggggcgccgt
     7081 cgaagaagtt cgcgggctgc ccgccgatgc cgccgagggt gctctcgtag cgcgtgatcc
     7141 ccgcctggag cgccgcgagg acgttcgcga ggccccagcc gcgcgtgacg tggaagtggg
     7201 cgacgtgctt tcccgggtcg gggatctcgt cgaggaccat cgcgtagtac gcgtaaaccc
     7261 ggtcgggcgg ggcggagccg tcatggtcgg cgtgctcgat gtcgtcggcg ccgatttcga
     7321 ggaagcgctt cgtgaacgtc acggcgtcct cgagccgcgt cggcccctcg a
//
LOCUS       DAOTEZ010000198         7361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_7168_length_7361_cov_6.103203, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000198 DAOTEZ010000000
VERSION     DAOTEZ010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7361
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7168_length_7361_cov_6.103203"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..1078)
                     /locus_tag="P5164_11305"
     CDS             complement(200..1078)
                     /locus_tag="P5164_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012694572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRY44521.1"
                     /translation="MVGGLRPFGATGLAVPPLGLGAGSVGDGSLDEAEAARLLHGALD
                     LGLSFVDTAPGYGLSEERIGRHLSGRRSSFVLSTKLGYGVPDVPDWTPECIARGVDQA
                     LSRLRTDVLDVAHLHSCPLETLLSSGVVEALLDAVRAGKVRVAAYSGENEALAWAVSS
                     GAFGSVQCSVNVCDQRSLALIAARGLGVIAKRPLANAPWNPAAAHPDDVAAAAYRCRW
                     TALRLDDLGRPPVELAIRFAAFAPGVSTAIVGSRRLEHLAELKALVAKGPLPPDILEE
                     ITTHFTTVGETWPGMI"
     gene            complement(1083..1859)
                     /locus_tag="P5164_11310"
     CDS             complement(1083..1859)
                     /locus_tag="P5164_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007792036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRY44522.1"
                     /translation="MSVVLTELSEGILTVTVNRPEKLNALNAEVIEELRRVFHEAKSN
                     AEVGALVLTGAGEKAFVAGADISGFPSLTPVDARDFARKGQAVLDLLETMGKPSIAAV
                     NGFALGGGCELAMACSMRVAATNARFGQPEVALGLIPGYAGTQRLPRLVGKGRALELI
                     LTGDPIPAEEAHRIGLVNRVVEPADLIPTCRALVAKILAKAPLAVRYALEAVNHGLEL
                     PFAEAELHEAALFGLCVATDDMKEGVAAFLGKRAAKFAGK"
     gene            complement(1870..2370)
                     /locus_tag="P5164_11315"
     CDS             complement(1870..2370)
                     /locus_tag="P5164_11315"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRY44523.1"
                     /translation="MRLIDPLLLEFDRECSGTRKCLERLPGEKLAWKPHEKSMSLGKL
                     AGHVATIPGWIGSALLADGFDLTSGMATPPLGTPEEIVAAFDASVKLAKGAMAQLDDA
                     KAMGEWTLAKGGQALFSMPRIALVRTILLNHSYHHRGQLTVYLRLLDVPVPGLYGPSA
                     DDDPFK"
     assembly_gap    2443..2452
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2453..3109)
                     /locus_tag="P5164_11320"
     CDS             complement(<2453..3109)
                     /locus_tag="P5164_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44524.1"
                     /translation="MKASLASALLAAALGASACRDAAPAPASAEPRRPGLSLREAYRP
                     PDDGVLTTPQVETFLKVREATLRVRATAAGNAPLEGEEGISDASEARAAEVRAARSLS
                     VRVDEYFWVRERILEAEAAAMTAKLNADVLALLEKTLASLRDRRPAAPDEASRRLLDE
                     QVASFEAEAARVRRESAEKEPESVRANLAVLAPYRSRISAIEDELAELDAAARREAAA
                     P"
     gene            3155..4831
                     /locus_tag="P5164_11325"
     CDS             3155..4831
                     /locus_tag="P5164_11325"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRY44525.1"
                     /translation="MLAKVGKYEILEKVGVGGFGTVYKGRDPYIKRTVAIKTCQSDED
                     ELRKRFFREAEFAGNLHHRNITTIYDFGLSDDGLPFIVQEFLTGEDLDRKIKRRDDLP
                     LAWRVRVLADICEGLHYAHTAGIVHRDVKPSNIRILEDGTVKIMDFGIAKSMVSESTL
                     TQTGITLGTASYLAPEQIRGEAVDARTDVFSLGILAYELLTLSRPFTGDHISTVLYKI
                     MNEAPPSPADVTPSLPPELVRIVEKALQKDRTNRYASCAEMKADLESVLTVLSGPVGA
                     PPRPAAPEVPSEAGPRGPGSARLDERVFRPAGGPSVAADLQLHTERSDAAPPVLPHPE
                     ETGSALKVFLGALAILVVVGGGLAWVLLGPGAKKAPSPELETDPVTTSTPAPAAVPPP
                     APTPGPTAPPPPATATPSPTTAPLGTALVSSNVYAQVAIDGRAQGGVLAAKPIRLAAG
                     RHVASFESGGFGPIRVPFEVKPGESVPVRADFPPLGQLWIHVNPDAVGAELFIDGERV
                     GRAGNAPLKKTVAAGERRVEARLPGFEPAEAVVSVYEQEKEDVRLELRRK"
     gene            4851..6506
                     /locus_tag="P5164_11330"
     CDS             4851..6506
                     /locus_tag="P5164_11330"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY44526.1"
                     /translation="MTRVRLLLAAAALLASLPALAQPASESLERDARAASRAGKFREA
                     AVKFDQAATAASDPQRRGRLRMQASYAALNAGNAADARESLTAAFADDPALEIVEQLF
                     TPEFRKLWQEVKAEVAKAAPAALADLAELKRTSEEKLKDGRFAEVAYDLGNYPHDKLD
                     RDAWALLAQAYERSGRSDQAALARRRALGENVPIAPLPEVAPPSPAGLPAAGSSATDL
                     LAFGREALNRGDALTAQAAANRVVEMEPSSSEGYRLLGDSYALRNERALADAMWRQSL
                     RLDERNEGTLLALAGVALEEKRFDEALDFLRRAVELNPRNADRLTTLGRRARAEGDTK
                     MARQVFAATVRALPSDVAVLSEYGAVLFEAGDVDAALEPLMTAASLAPDRAPVRASLA
                     AALRRKGSLPEAEREYREALRADPAWLPALRGLGVLLLATDRSADAVEPLRTALAAAP
                     ADSDTLLALARAQRLSGDLAGAGATLAAAASGRDATLLNEAGAVAYAEGRWGDAQALF
                     EKARAADPGLAVAAGNLEKAAAAARVVAALAAAPPPPAPAPKR"
     gene            complement(6523..7134)
                     /locus_tag="P5164_11335"
     CDS             complement(6523..7134)
                     /locus_tag="P5164_11335"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HRY44527.1"
                     /translation="MTEAAPWILEIRRRLERERVVPDVVREIAEGTPDARPAAVLVPL
                     YVRERELWTLLTKRSETVESHRGQIAFPGGMEALDDAGPWETAIRETEEEIGLPRKAI
                     LRIGELPGVTTFTGFRIRAFVGAIPYPFDVRHDPREVDSVIQVPIRALMGPTLVEERA
                     VTWKGRSIPTPVYHLKGHVIWGATAFLVSSLLEALVDEGRPPA"
     gene            complement(7131..>7361)
                     /locus_tag="P5164_11340"
     CDS             complement(7131..>7361)
                     /locus_tag="P5164_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_148260015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44528.1"
                     /translation="GVKMEIGFGSQIRSYVLAPYRLAKDHRTGFEMGDVDRVLDGDLD
                     GYVEAWLQMAVKKGEKHARERKSADALLEEEP"
BASE COUNT         1025 a         2694 c         2670 g          962 t
ORIGIN      
        1 tccgcgagca ggccatcggg agaagcggac gcgctgtttg acgagcgaag cgaggagttc
       61 gcggccgcgc cgatggcccg cgcagcggag ccgatgcggg tcgcgcgcgc gcagggctcg
      121 ccgtgggatg cctctcctgg ccgcgactcg ggcggcgcga cgaaatggtc agagggctct
      181 cgggttctcg gggggggggt cagatcatcc cgggccaggt ctcgccgacg gtggtgaagt
      241 gcgtcgtgat ctcctcgagg atgtccggcg ggagcgggcc tttcgcgacc agggccttca
      301 gctcggcgag gtgctcgagg cggcgggagc cgacgatggc ggtcgagact ccgggggcga
      361 aggcggcgaa gcggatggcg agctcgacgg gagggcgacc gaggtcgtcg aggcggaggg
      421 cggtccagcg gcatcggtag gccgcggcgg ccacgtcgtc cgggtgggcg gcggcggggt
      481 tccagggcgc gttcgcgagg gggcgcttgg cgatgacgcc taggccgcgg gccgctatga
      541 gggcgaggga gcgctggtcg cagacgttga ccgagcactg gacggagccg aaggcgccgg
      601 aggagacggc ccacgcgagc gcttcgttct cgccggagta ggcggcgacg cggacctttc
      661 cggcgcggac ggcgtcgagg agggcctcga cgacgccgga ggagaggagc gtctcgagcg
      721 ggcaggagtg gaggtgtgcg acgtcgagga cgtcggtgcg gagccgggag agggcctggt
      781 cgacaccgcg ggcgatgcac tccggagtcc agtcgggaac gtcggggacg ccgtatccga
      841 gcttcgtcga gaggacgaat gaggagcgac ggcccgagag gtggcggccg atccgctcct
      901 cggagaggcc gtagccgggc gcggtgtcga cgaaggagag gccgaggtcg agcgcgccgt
      961 ggagaagtcg cgcggcttcg gcctcgtcga gggacccgtc cccgacggac cccgcgccga
     1021 ggccgagggg cggaacggcg agccccgtcg caccgaacgg acggagcccg ccgaccacgg
     1081 ggtcacttcc ccgcgaactt cgccgcgcgc ttcccgagga aggcggcgac gccttctttc
     1141 atgtcgtccg tcgcgacgca gaggccgaag agggccgcct cgtggagctc ggcctcggcg
     1201 aacgggagct cgaggccgtg gtttaccgcc tcgagcgcat agcggacggc gagcggcgcc
     1261 ttcgcgagga tcttcgccac gagggcgcgg cacgtcggga tcaggtcggc cggctcgacc
     1321 acgcggttca cgaggccgat ccggtgcgcc tcctcggccg ggatcggatc gcccgtgagg
     1381 atcagctcga gcgcccgccc ctttcccacg aggcgcggga ggcgctgcgt acccgcgtag
     1441 cccgggatca ggccgagggc gacctcgggc tggccgaagc gagcgttcgt cgcggcgacg
     1501 cgcatcgagc aggccatcgc gagctcgcag ccgccgccga gggcgaagcc gttgaccgcg
     1561 gcgatcgagg gcttccccat cgtctcgagg aggtcgagga cggcctgccc cttccgcgcg
     1621 aagtcgcgcg cgtcgaccgg ggtgagcgac gggaacccgg agatgtcggc ccccgcgacg
     1681 aaggccttct cccccgcacc tgtcaggacg agggccccga cctcggcgtt cgacttcgcc
     1741 tcgtggaaga cccggcgcag ctcctcgatc acctcggcgt tcagggcgtt cagcttctcc
     1801 ggccggttga cggtgacggt gagtatcccc tcggacagct cggtcaggac gacggacacg
     1861 ctcgcctcct cacttgaacg ggtcgtcgtc ggcgctcggg ccgtagaggc ccgggaccgg
     1921 gacgtcgagc agccgcaggt agacggtgag ctgcccgcgg tggtggtagg agtgattgag
     1981 gaggatcgtc cggacgagcg cgatacgcgg catcgagaag agggcctgac cgcccttcgc
     2041 gagggtccac tcccccatcg ccttcgcgtc gtcgagctgc gccatcgccc ccttcgccag
     2101 cttcacggag gcgtcgaagg cggcgacgat ctcctccggc gtgccgagcg gcggggtcgc
     2161 cattccggac gtcaggtcga agccgtccgc gaggagcgcg ctgccaatcc agcccgggat
     2221 cgtcgccacg tggccggcca gcttcccgag gctcatcgac ttttcgtggg gcttccacgc
     2281 gagcttttcg ccaggaaggc gttcgaggca cttgcgggtg ccggagcact cgcggtcgaa
     2341 ctcgaggagg agcggatcga tgaggcgcat ggtcgttctc ctctggggct tcgatttccg
     2401 gtgacgggaa gaacgagtct agcagcgcgt cggggcccta ccnnnnnnnn nngggagcgg
     2461 cggcctcgcg ccgggccgcg gcgtccagct cggcgagctc gtcctcaatc gccgagatcc
     2521 gcgaccggta cggggcgagg accgcgaggt tcgcgcggac ggactccggc tccttctcgg
     2581 ccgactcgcg gcgcacccgg gccgcttccg cctcgaagct cgcgacctgc tcgtcgagga
     2641 ggcggcgcga ggcctcgtcg ggcgcggccg gccgccggtc ccgcagggac gcgagcgtct
     2701 tctccagcag ggcgaggacg tcggcgttca gcttcgcggt catcgccgcg gcctcggcct
     2761 cgaggatccg ctcccgcacc cagaagtact cgtcgacgcg gaccgagagc gaacgggccg
     2821 cccggacctc cgcggcgcgc gcctccgagg cgtcggagat cccctcctcc ccctccagcg
     2881 gcgcgttccc cgcggccgtg gcccggaccc ggagcgtcgc ctcgcgcacc ttgaggaacg
     2941 tctccacctg gggcgtcgtc aggacgccgt cgtcgggagg ccgatacgcc tcgcgcaggg
     3001 agaggcccgg ccgccgcggc tccgccgatg cgggggccgg ggcggcgtcc cggcacgcgc
     3061 tcgccccgag cgcagcggcg aggagggcgg aagcgagcga agccttcacg ggagaggaat
     3121 atacgcgagc gcatgttacc ctcgccggac gggcatgctc gcgaaggtcg gcaagtacga
     3181 gatcctcgag aaggtcggcg tcggcggctt cgggaccgtc tacaagggcc gggacccgta
     3241 catcaagcgg accgtcgcca tcaagacctg ccagagcgac gaggacgagc tccggaagag
     3301 gttcttccgg gaggccgagt tcgccgggaa cctgcaccac aggaacatca cgacgatcta
     3361 cgacttcggc ctgagcgacg acggcctgcc gttcatcgtc caggagttcc tgacgggcga
     3421 ggacctcgac cggaagatca agcggaggga cgacctcccg ctcgcgtggc gggtccgcgt
     3481 cctcgccgac atctgcgagg ggcttcacta cgcccacacg gccgggatcg tgcatcgcga
     3541 cgtgaagccg tccaacatcc ggatcctcga ggacgggacc gtgaagatca tggacttcgg
     3601 catcgccaag tcgatggtct ccgagtcgac gctgacgcag accggcatca cgctcggaac
     3661 ggcgtcctac ctggccccgg agcagatccg gggcgaggcc gtcgacgccc gcaccgacgt
     3721 cttctccctc ggaatcctcg cctacgagct cctgaccctc tcccgcccgt tcaccgggga
     3781 ccacatctcg acggtcctct acaagatcat gaacgaggct cctccgtccc cggcggacgt
     3841 gacgccgagc cttcccccgg agctcgtccg gatcgtcgag aaggcgctgc agaaggaccg
     3901 gacgaaccgg tacgcttcct gcgccgagat gaaggcggac ctggagtcgg tcctgacggt
     3961 cctctcgggg ccggtcggcg cccctccccg tcccgccgcc cccgaggtcc cgtccgaggc
     4021 cggaccgcgc ggtcccggaa gcgcgcggct cgacgagagg gtcttccggc cggccggcgg
     4081 accgtccgtc gccgccgacc tgcagctcca caccgagcgc tcggacgcgg ccccgcccgt
     4141 tctgcctcat cccgaggaga cgggaagcgc cctcaaggtg ttcctcgggg cgctcgcgat
     4201 cctcgtcgtc gtcggcggag gcctcgcctg ggtcctgctg ggacccgggg cgaagaaggc
     4261 cccgtcgccg gagctcgaga ccgatccggt cacgacctcg acgccggcgc ccgcggccgt
     4321 tccgccgcct gcgcccacgc cggggccgac cgcgccgccg ccgcccgcca cggcgactcc
     4381 ctcgccgacg accgcgcccc tcgggacggc cctcgtctcc tcgaacgtct acgcccaggt
     4441 cgcgatcgac gggcgcgccc agggcggcgt cctggccgcg aagccgatcc gcctcgccgc
     4501 cggccggcac gtcgcgtcgt tcgagagcgg gggcttcggc ccgatccgcg tcccgttcga
     4561 ggtgaagccc ggcgagagcg tccccgtccg cgccgacttc ccgcccctcg gacagctctg
     4621 gatccatgtc aaccccgacg ccgtcggggc ggagctcttc atcgacgggg aacgggtcgg
     4681 gagggccggg aacgcgccgc tcaagaagac ggtcgccgcc ggagagcggc gcgtcgaggc
     4741 gcggctgccc ggcttcgagc ccgcggaagc ggtcgtctcg gtctacgagc aggagaaaga
     4801 ggacgtccgg ctggagcttc ggcgaaagta ggagccctta gaatcggcgc gtgacccgcg
     4861 tccgactcct cctcgccgcc gccgcgctcc tcgcctccct tcccgctctc gcgcagccgg
     4921 cgtccgagtc cctggagagg gacgcgcgcg cggcctcgcg ggccgggaag ttccgcgagg
     4981 ccgccgtgaa gttcgaccag gcggcaaccg ccgcgtccga tccgcagcgg cggggccgac
     5041 tgcggatgca ggcgtcctat gccgctctga acgccgggaa cgcggcagac gcccgggaga
     5101 gcctgacggc ggccttcgcc gacgaccccg cgctcgagat cgtcgagcag ctcttcaccc
     5161 ccgagttccg gaagctctgg caggaggtga aggccgaggt cgcgaaggcc gctccggccg
     5221 ctctcgccga cctcgccgag ctgaagcgga cctcggagga gaagctcaag gacggccgtt
     5281 tcgccgaggt cgcctacgac ctcggcaact acccgcacga caagctcgac cgggacgcct
     5341 gggcgctcct cgcgcaggcc tacgagcggt ccggtcgttc cgaccaggcc gccctcgccc
     5401 ggcgccgcgc cctcggagag aacgtcccca tcgcgccgct cccggaggtc gctccgccga
     5461 gcccggccgg ccttcccgcc gcgggttcgt cggccacgga cctcctcgcg ttcggacgcg
     5521 aagcgctgaa ccgcggcgat gccctcaccg cccaggccgc cgcaaaccgc gtcgtggaga
     5581 tggagccctc ctccagcgag ggctaccggc tcctcggcga ttcgtacgcg ctccggaacg
     5641 agagggccct cgccgacgcc atgtggcgcc agtcgctccg tctcgacgag cgcaacgagg
     5701 gaacgctcct cgccctcgcc ggcgtcgccc tcgaggagaa gcggttcgac gaggcgctcg
     5761 acttcctccg gcgcgccgtc gagctgaacc cccggaacgc ggaccgcctg acgacgctcg
     5821 ggcggagggc ccgggccgaa ggcgacacga agatggcccg gcaggtcttc gccgcgaccg
     5881 tccgggccct cccgagcgac gtcgcggtcc tctccgaata cggggccgtc ctcttcgagg
     5941 cgggtgacgt cgacgccgcc ctcgagccgc tcatgacggc ggcctcgctg gcccccgacc
     6001 gggcccccgt ccgggcgagc ctcgcggccg cgctccggag gaaaggctct ctccccgagg
     6061 ccgagcggga gtaccgggag gcgctccggg cggaccccgc ctggctcccc gccctgcgcg
     6121 ggctgggcgt cctcctcctc gccaccgacc ggtccgccga cgcggtggag ccgctccgga
     6181 cggccctcgc ggccgcgccc gccgactccg acacgctcct cgcgctggcc cgcgcccagc
     6241 gactctccgg cgacctcgcc ggggccggag ccacgctggc ggccgccgct tccggaaggg
     6301 acgcgacgct cctgaacgag gcgggcgcgg tcgcctacgc cgaaggacgg tggggagacg
     6361 cgcaggccct cttcgagaag gcgcgggcgg ccgacccggg cctcgccgtc gccgccggga
     6421 acctcgagaa ggcggccgcg gctgcgcgcg tcgtcgcggc gcttgccgcc gctcctcctc
     6481 cgcccgcccc ggcgccgaag cgctgaagcg gccgccccgc cgtcaggccg gcggccgccc
     6541 ctcgtcgacg agcgcctcga ggaggctcga gacgaggaag gccgtcgcgc cccagatcac
     6601 gtgccccttc aggtggtaga cgggggtcgg gatcgatcgc cccttccacg tcacggcccg
     6661 ctcctcgacg agcgtcggac ccatcagcgc gcggatggga acctgaatca ccgagtcgac
     6721 ctcgcgcgga tcgtgccgca cgtcgaacgg gtacgggatc gccccgacga acgcgcggat
     6781 ccggaacccc gtgaacgtcg tcacgcccgg cagctcgccg atccggagga tcgccttccg
     6841 gggcaggccg atctcctcct ccgtttcccg gatcgccgtc tcccacgggc cggcgtcgtc
     6901 gagcgcctcc atccctcccg ggaacgcgat ctgcccgcgg tggctctcga ccgtctcgct
     6961 ccgcttcgtc aggagcgtcc agagctccct ctccctcacg tagagaggga cgaggacggc
     7021 cgcgggacgg gcgtcgggcg tcccctcggc gatctcgcgg accacgtcgg ggacaacccg
     7081 ctcgcgctcg aggcgccgcc ggatctcgag gatccaggga gcggcctcgg tcacggctcc
     7141 tcctcgagga gcgcgtccgc gctcttcctc tcgcgggcgt gcttctcccc cttcttcacg
     7201 gccatctgga gccaggcctc cacgtagccg tcgaggtccc cgtcgaggac ccggtcgacg
     7261 tcccccatct cgaagccggt ccggtggtcc ttcgcgaggc ggtacggggc gaggacgtag
     7321 gagcggatct gcgagccgaa gccgatctcc atcttcacgc c
//
LOCUS       DAOTEZ010000199         7350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_7184_length_7350_cov_5.329815, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000199 DAOTEZ010000000
VERSION     DAOTEZ010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7184_length_7350_cov_5.329815"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..399
                     /locus_tag="P5164_11345"
                     /pseudo
     CDS             1..399
                     /locus_tag="P5164_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236026.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            457..2070
                     /locus_tag="P5164_11350"
     CDS             457..2070
                     /locus_tag="P5164_11350"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRY44529.1"
                     /translation="MVFGYDRFDSTRASNNWQSGSSWVLNSTSSQFDGTNLYPVIDEN
                     SYFQYYPIFELTKGSSLVTHSVFVNDSWRLNDNFSFNVGVRWDKNDATDAAGNTTAND
                     NAFSPRLSATWDPSGNGRLRLSASYATYVGQLQEGIAGSGATLAGTPSSFYYYWYGDP
                     INTNETGPWLTSAQVIEKMLAALGVTRLNQLPNVSADQASVNGFSTIIKDPLSSPKVD
                     EVVLGIGGSLFGNLTYRVDGVYRKFKDFYGVNRTVPNGTVTFAPTGDVLDLGYVINSN
                     DLEREYTGLHTSLAWQKGPLSLAATWTFSRLYGNIIGETSGSGPVTAGLQEYPEFWNA
                     SWYAPKGHLPADQTHRVRFVGSYDLQLGPITITPGLIETLDTGTAYGAWSLTANYGGI
                     ASYPYVTNPGYAMPPNRVDYYFTPRDAYRTDTVTRTDLSLNLSGRIGPVEIYVQPQIW
                     NVFNEEGVQTVNQSVSVGTSSSASASTGLKRWNPFTTAPIECPQGSSAATCSAMGANW
                     RKGANFGKPTSATHYQIPQRFLVSLGIRF"
     gene            2429..3403
                     /locus_tag="P5164_11355"
     CDS             2429..3403
                     /locus_tag="P5164_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRY44530.1"
                     /translation="MRVLIIGGIQFMGREAARRLAARGDEVTVLHRREGHDLGPAVRE
                     LRADRADLPRVERLLREQAFDAIVDTAYDWVNGTPPGHVEAAARAAGPQLTRYVFVSS
                     IAAYGGGLDRDEDDPLAPDDVPNPYARHKAAAERALFRMHAENGFPAVTLRPPFVYGP
                     RQPFYREAFFWDRMRDGRPVLLPDGGTTLMQWVCVDDVAEACVRALDEPGAVGQAFNL
                     GHPPVSQRAFVEALGRAAGVAPALVSAPREAILAEGGNAFAGDLYFGEVLDLPPMTET
                     VGKVTRVLGLSPGDFEEGLRRSREWYLAQPRRPVDYAFEDRVLARLGG"
     gene            3606..3770
                     /locus_tag="P5164_11360"
     CDS             3606..3770
                     /locus_tag="P5164_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44531.1"
                     /translation="MPDALGLLVVEGRALDLAQPHQAGVPGLLLRVQGSGSIAGSTLR
                     GRGKRSSSSA"
     gene            complement(3916..5118)
                     /locus_tag="P5164_11365"
     CDS             complement(3916..5118)
                     /locus_tag="P5164_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HRY44532.1"
                     /translation="MKRPSFSRVAVLALSFGLAAGSVAGDDRRPGHREPLVIAHRGAS
                     GYLPEHTLEAYALAIDQGADFIEPDLVMTRDGHLVVRHEVNIAATTDVAAHPEFARRQ
                     RTMAVDGVPEEGWFVSDFTLAELRTLRIVQRYPDRAHVFDGRYRIPTFEEVLDLARRR
                     SWEERRPIGVYPETKHPTLHRSLGLPLEPAVVRALKRCGWDRRDAPVFLQSFEPSSLQ
                     RLERMTDVRLVQLVDASDVAPDGSLVFEPPFDRPYDWTVSADPRLRARRFDFLVSDEG
                     LREVRRYADVISPWKRFVVSSHAVDRNGDGTIGDENGDGAVNEADRALLPPTDLVERA
                     HDLGLAVHLWTLRSEPRFLVSDYAGNPVNEYLQLLRLGVDGLFSEFPDTAVTARALLR
                     LEEPGPGR"
     assembly_gap    5701..5800
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6141..>7350)
                     /locus_tag="P5164_11370"
     CDS             complement(6141..>7350)
                     /locus_tag="P5164_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007690833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRY44533.1"
                     /translation="EILQGLGFPDEVIDGDVGALSGGWKMRVAMARILLGRPDVLLMD
                     EPTNHLDIESILWLERFLHDYPGTLLMTSHDRDFLNRIVTKIVEVDGGEIFTWSGDYD
                     FYERERTLREAQREAAYARQQAMLAKESRFIERFSAHAAKAAQVQSRVKKLEKIEQIE
                     MPRRRKVVRFDFRPPPRSGDDVAALSGVGKRYGSRVVYDGFDFLVRRGERWCVMGRNG
                     AGKSTLLKMVSGALAPDAGTVKLGASLKLGYFAQQSLDLLDPTLTVIEQLQKDFPLEG
                     LGVLRTLAGAFQFSGDDVDKPVRVLSGGAKSRLVLARMLFDPPNFLVLDEPTNHLDLA
                     TKEMLVEALHAFEGTMLFVSHDRTFLRGLANRVLEIGGEDGTGKPVTYGGTYEEYVAR
                     SGHEAPGVHA"
BASE COUNT         1123 a         2513 c         2623 g          991 t
ORIGIN      
        1 cgcgccctgc aggtggcggt cacccagccg attccggacc tgatcctgct cgatatcatg
       61 atgcccggca tggatggcta cgaaatcctg cgccacctgc gtcagatacc ggaaacccgc
      121 gacattccgg tcattttcgt caccgccatg tcagccgacg aagatgagga ggccggcctg
      181 cacctcggtg ccgtcgatta catcaccaag ccgatccgcc cggccattct cagcgcctgc
      241 gtgcgcaccc acctcgccct cacacaggcc cggcagcaac tggccaacca gaatgcccgg
      301 ctcgaatcgc aggtccggca gcgtacgctc gaactggagc tgatcaagga agtcaccatg
      361 caggcgctgg ctacgctggc cgagaagcgc gacaacgagg acgtcctggt caaggggacg
      421 tacttcctgt cgacgaaggg tctcggctcg cacaacatcg tcttcggata cgaccgcttc
      481 gactcgacgc gcgcctcgaa caactggcag tcggggagct cctgggtcct caactcgacc
      541 tcctcccagt tcgacgggac gaacctctac ccggtcatcg acgagaacag ctacttccag
      601 tactacccga tcttcgagct gacgaagggg agcagcctcg tcacgcactc cgtcttcgtc
      661 aacgactcct ggcgactcaa cgacaacttc tcgttcaacg tcggggtgcg gtgggacaag
      721 aacgacgcca ccgatgcggc ggggaacacg accgcgaacg acaacgcctt cagcccgcgc
      781 ctctcggcga cctgggaccc gagcgggaac ggcaggctcc gcctctctgc gagctatgcg
      841 acctacgtcg gtcagctcca ggaggggatc gcggggagcg gggcgaccct ggccgggacg
      901 ccctcgagct tctactacta ctggtatggc gacccgatca acaccaatga gaccgggccg
      961 tggctcacga gcgcccaggt catcgagaag atgctggccg cgctcggggt gacgagactc
     1021 aaccagctgc cgaacgtcag cgccgaccag gcgagcgtca acgggttcag cacgatcatc
     1081 aaggacccgc tctcctcgcc gaaggtcgac gaggtcgtcc tcgggatcgg cggctcgctc
     1141 ttcgggaacc tcacgtatcg cgtcgacggc gtctaccgca agttcaagga cttctacggt
     1201 gtgaatcgga cggtgccgaa cggcacggtg acgttcgctc cgacggggga cgtcctcgac
     1261 ctcggctacg tcatcaactc gaacgacctc gagcgggagt acacgggcct ccacacctcc
     1321 ctcgcctggc agaaggggcc cctcagcctc gccgcgacgt ggaccttctc gaggctctac
     1381 gggaacatta tcggagagac gagcggctcc gggccggtca ccgcgggcct gcaggagtac
     1441 cccgagttct ggaatgcctc ctggtatgcg ccgaaggggc acctgcccgc ggaccagacg
     1501 catcgcgtgc gcttcgtcgg gtcgtacgac ctccagctcg ggccgatcac gatcaccccg
     1561 ggcctcatcg agacgctcga caccggaacg gcctatggcg cctggagcct gacggcgaac
     1621 tacggcggca tcgcctccta cccctacgtc acgaaccccg gctacgccat gccgcccaac
     1681 cgggtcgatt actacttcac gccgcgggac gcctatcgga cggacaccgt gacgcgaacg
     1741 gacctctcgc tgaacctgag cgggaggatc ggaccggtcg agatctacgt ccagccgcag
     1801 atctggaacg tcttcaacga ggagggcgtc cagacggtga accagtcggt gagcgtcggg
     1861 acgtcgtcga gcgcgagcgc gagcacgggg ctgaagcggt ggaacccctt cacgacggcc
     1921 ccgatcgagt gtccccaggg ctcctcggcg gcgacgtgct ccgcgatggg ggcgaactgg
     1981 cgaaagggcg cgaacttcgg caagccgacg agcgcgaccc actatcagat cccgcagcgc
     2041 ttcctcgtct ccctgggcat ccgcttctag cggcggaccc tccctcccgc cgattccacc
     2101 ggcccccgga ccctcgggtc cgggggcctt tcccctctcc agcgcgcgct tccgggctga
     2161 cgcggtcggc cccgccggcc tggagcgcga cgccggcgac cgcatcttcc ccgcgaccga
     2221 gggcgaggag agccaggccg cagagcttcc acccgtcgcg gcctgcgggg cggtggctct
     2281 ccgggagcgg ctggaggaag gctcggtgct cgcgcgccgc gaagacgagg atcgcggacg
     2341 tcccggcgag gacgaggagg accccgatcg cgcgcgagag cggtacgagg cacggggggc
     2401 acgcctcggc cgctggtacg atttcgagat gcgagtcctg atcatcggcg gaatccagtt
     2461 catgggccgc gaggccgccc gccgcctcgc ggcgcgtgga gacgaggtga cggtcctcca
     2521 ccggcgcgag ggccacgacc tcggccccgc ggtccgcgag ctccgtgcgg accgggccga
     2581 cctgcctcgg gtggagcgcc tcctccgcga gcaggcgttc gacgcgatcg tcgacacggc
     2641 ctacgactgg gtgaacggga cgccgcccgg gcacgtcgag gcggcggcgc gtgcggcggg
     2701 accgcagctg acccgctacg tgtttgtctc gagcatcgcg gcctacgggg gcgggctcga
     2761 ccgggacgag gacgacccgc tcgcgccgga cgacgtgccg aacccgtacg cgagacacaa
     2821 ggcggccgcc gagcgggccc tcttccggat gcacgccgag aacggcttcc cggcggtcac
     2881 gctccgcccg cccttcgtct acgggccgag gcagcccttc taccgcgagg cgttcttctg
     2941 ggaccggatg cgcgacggcc gccccgtcct cctccccgac ggcggcacga cgctgatgca
     3001 gtgggtctgc gtcgacgacg tggcggaggc ctgcgtgcgc gctctcgacg agcccggggc
     3061 cgtgggccag gcgttcaacc tcggccaccc gccggtctcg cagcgcgcgt tcgtcgaggc
     3121 tctcgggcgg gcggcgggcg tcgcccccgc cctcgtctcc gcgccgcggg aggcgatcct
     3181 cgcggagggc gggaacgcct tcgcgggcga cctctacttc ggcgaggtcc tcgacctgcc
     3241 gccgatgacc gaaacggtcg ggaaggtgac gcgcgtcctg ggcctctcgc cgggcgactt
     3301 cgaggagggg cttcgccgct cccgcgagtg gtacctcgcc cagccgaggc gaccggtcga
     3361 ctacgcgttc gaggaccggg tcctggctcg cctcggaggg tgacgccgcg gcccgggagg
     3421 gctcgcctac gcgtggacgc cgggcgctcc tctgcaaggc gaggttccgg ggcgagccct
     3481 gaggccgggc gacggacgcc ccctctccga cgggccgcgg actccgcgag aaagccgatc
     3541 gacagcccct tcgcggagtc cttcccgaac ttcttcatca ggccggcggc gaccagggtg
     3601 tccctgtgcc ggacgcgctc ggcctcctcg tggtcgaggg gcgcgccctc gatctggcgc
     3661 agccgcacca ggccggcgtc cctggcctcc tgctccgcgt ccaggggtcc ggatcgatcg
     3721 ccgggtcgac cctgcggggc agggggaaga ggagctcgtc ctcggcgtga tggtcgtgcg
     3781 gcgccagccg cgcgaagagc tcgatgcgct tccgcgcgag cgcgggagag gcgccgcccg
     3841 gatcgaggcg ggcgaaggcg ccggacggcg accacacggg cgccgccgtc ggcgccgtgc
     3901 cggccggggc ctcgactaac ggccgggacc cggctcctcc aggcgcagga gcgccctcgc
     3961 ggtgaccgcc gtgtcgggga actccgagaa gagcccgtcg accccgaggc ggaggagctg
     4021 gaggtactcg ttcacggggt tgccggcgta gtcggagacg aggaatcgcg gctcgctccg
     4081 gagggtccag aggtggacgg ccagaccgag gtcgtgggcc cgctccacga ggtcggtcgg
     4141 gggcaggaga gcccggtccg cctcgttcac ggcgccgtcg ccgttctcgt cgccgatcgt
     4201 cccgtcgccg ttccggtcga ccgcgtggga gctgacgacg aaccgcttcc aggggctgat
     4261 cacgtcggcg tatctccgga cctcgcggag cccctcgtcg ctcaccagga agtcgaaacg
     4321 gcgggcgcgc agacgcgggt cggcggacac ggtccagtcg tacggccgat cgaagggcgg
     4381 ctcgaagacg agcgagccgt ccggcgcgac gtcgctcgcg tcgacgagct gcacgagccg
     4441 gacgtcggtc atccgctcga gccgctgcag cgaggagggc tcgaacgact ggaggaagac
     4501 cggggcgtct cggcgatccc agccgcagcg cttgagcgcg cggacgacgg cgggctcgag
     4561 cgggaggccg agcgagcggt gcagcgtcgg gtgcttcgtc tccgggtaga ccccgatcgg
     4621 ccgccgctcc tcccacgagc gccgccgggc gaggtcgagg acctcctcga acgtggggat
     4681 ccggtaccgg ccgtcgaaga cgtgcgcgcg gtccgggtac ctctggacga tgcggagcgt
     4741 gcgcagctcc gcgagggtga agtccgagac gaaccaccct tcctccggca ccccgtctac
     4801 cgccatcgtc cgctgccgtc gcgcgaactc cgggtgggcg gccacgtctg tcgtcgccgc
     4861 gatgttcacc tcgtgacgga cgacgaggtg gccgtccctc gtcatcacga ggtccggctc
     4921 gatgaagtcg gcgccctggt cgatcgccag cgcgtaggcc tcgagagtgt gctcgggcag
     4981 gtagcccgaa gcgccgcggt gggcgatgac gagcggctcg cggtggccgg gccggcggtc
     5041 atcgcccgca acggaaccgg cggcgaggcc gaatgagagg gcgagaacgg ccacgcggga
     5101 gaaggaagga cgcttcacgg ggtacctccg ggacgtaccg gtctcatcct acccagtccc
     5161 gggggcgtcg agggtcggga agtccgaggg ataggaggtc agatatcggg cggcgacgcg
     5221 ggtgaaggcc gaagcgggta cgccggacaa cccgggggcg gcaccgcggc ggtgacgccc
     5281 ccgggcgcgg ggccccggga gcatcggtgg ccacgcgggg ccggtgctcg aggcggggct
     5341 gggtgaggaa ggcgaccgcg cggatcacgc cgcggcatcg gggacggacg aggggatcga
     5401 cctcgaagac ccagcggatg agccgggccc atccgcggcg aagggcggcc gaagaggaga
     5461 gcccgcggtg ggtggcggcg gagggggaga ggccgttgtc gaggcgagcg gctctgctgg
     5521 ccgtgcctcg gacgacgttc gatcaggcgc cgtagccgtg gaccaggtgt ctcctcgggt
     5581 cggggacgtg cgagagaagg cgggcgacga aatcgagggc gtcgcaaggc ccggccctct
     5641 cgtcgtcgtg gccgtcgcgg ggccggtacc tggcggtctg cgagcccggc gagaagcgca
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccgtagaagg gactcgatcg
     5821 gcacgcacag gggcgtctcg gcgggcctgc gcgcccggta aagggctgcc tccacggcac
     5881 gaggccaggg cggacctcgc gccggagggc cggagacaga gtgcccgtgg gacaggggct
     5941 tcgacgcggg tggccgatgc aggtcggggc cggcgagaca ggagaacctg acgaagacgg
     6001 tgcgcgacga acgcgaaaac gccgccgtgt ccggggatcg acgacgacga cgaaaccggt
     6061 ccgcgctgac ggggctgcct gtcggcgacc cgtgaggctc ctccacgccg tcactgcggc
     6121 cccggcccgg gtgagcccgc ctacgcgtgg acgcccgggg cctcgtggcc cgagcgcgcg
     6181 acgtactcct cgtaggtgcc gccgtaggtc acgggcttgc cggtgccgtc ctcgccgccg
     6241 atctcgagga cgcggttcgc gaggccgcgg aggaaggtcc ggtcgtggga gacgaagagc
     6301 atcgtcccct cgaacgcgtg gagcgcctcg acgagcatct ccttcgtcgc gaggtcgagg
     6361 tggttcgtcg gctcgtcgag gacgaggaag ttcggcgggt cgaagagcat ccgcgcgagg
     6421 acgagccgcg acttcgcgcc gcccgagagg acgcggaccg gcttgtcgac gtcgtccccc
     6481 gagaactgga acgcgccggc gagcgtccgg aggacgccga gcccctcgag cgggaagtcc
     6541 ttctggagct gctcgatcac cgtgagcgtc gggtcgagga ggtcgagcga ctgctgggcg
     6601 aagtagccga gcttcaggct cgcgccgagc ttcaccgtcc ccgcgtcggg cgcgagcgcg
     6661 cccgagacca tcttcaggag cgtcgacttg ccggcgccgt tacgacccat gacgcaccag
     6721 cgctcgccgc ggcggacgag gaagtcgaag ccgtcgtaga cgacgcgcga gccgtagcgc
     6781 ttgccgacgc cggagagcgc agcgacgtcg tcgcccgagc ggggcggcgg acggaagtcg
     6841 aaccgcacga ccttgcggcg tcgcggcatc tcgatctgct cgatcttctc gagcttcttc
     6901 acgcggctct gcacctgcgc ggccttcgcc gcgtgggccg agaagcgctc gatgaagcgc
     6961 gactccttcg cgagcatcgc ctgctgccgg gcgtaggccg cctcgcgctg cgcttcgcgg
     7021 agcgtccgct cgcgctcgta gaagtcgtag tcgcccgacc aggtgaagat ctcgccgccg
     7081 tccacctcca cgatcttcgt gacgatccgg ttcaggaagt cccggtcgtg gctcgtcatc
     7141 aggagagtcc cggggtagtc gtgcaggaag cgctcgagcc agaggatcga ctcgatgtcg
     7201 agatggttcg tcggctcgtc catcaggagg acgtcgggcc tcccgaggag gatccgggcc
     7261 atcgccacgc gcatcttcca gccccccgag agcgcgccga cgtcgccgtc gatcacctcg
     7321 tcggggaagc cgagcccctg gaggatctcc
//
LOCUS       DAOTEZ010000200         7335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_7201_length_7335_cov_5.994780, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000200 DAOTEZ010000000
VERSION     DAOTEZ010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7335
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7201_length_7335_cov_5.994780"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /locus_tag="P5164_11375"
     CDS             <1..1229
                     /locus_tag="P5164_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRY44534.1"
                     /translation="DVTVEALAVATNTPPNGAFRGFGAPQTIWAIERHLDRVARTLGT
                     DPVALKRKNLLRLGDTTATGQTLKESVGVEECVEKAMAASGYVEKRERGPVVTGRTAR
                     GVGAAIFLHGAGFTGSGEKYLKGKVAVDLLPGGRARVRTGSTDIGQGTETVFRQIAAD
                     GLGIGVENVDFAVPSTANVPDSGPTVASRTVMVVGSIVDAAAKELRARLEAEEKARGA
                     TRSAAWERLAADPARATSLVQYEPPPGVVWDDATYSGDAYPAYGWACDVAEVEVDLDT
                     FETKVTGFWAAVDVGRVINPTLCAGQVEGGTLQAIGWALTEKLVTKDGKILNPRMTNY
                     IVPTSLDAPPFTTTLVEAAYSRGPGGAKGVGELPMDGGAPAVAAAIEHATGLVLDRVP
                     MLPEDLFEASVKGGAR"
     gene            1232..1708
                     /locus_tag="P5164_11380"
     CDS             1232..1708
                     /locus_tag="P5164_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRY44535.1"
                     /translation="METNITLTVNGSVVTLEAPPAARLLDVLRERLGLTGTKEGCGEG
                     ECGACTVLLDGVPVNACLVAFGQCDGREVTTVEGLGSTAHLTPLQEAMVTEGGAQCGI
                     CTPGMLLSAEALLREEPRPTEPQIREAIAGNICRCTGYQRIVNAVNEAARRREARS"
     gene            1705..2595
                     /locus_tag="P5164_11385"
     CDS             1705..2595
                     /locus_tag="P5164_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HRY44536.1"
                     /translation="MSVAGLALHPFSTPKTLDEALALLAERSARAEKTVLLAGGTDWF
                     VERHVAPAEEAGVLPLVVDVSRLDALRGIAVEGETLHIGAAATYLEIRKDARVAARCP
                     LLAATARDVGALQIQARGTLGGNLASGSPAADGVAALAALDATLLLRSIRGARRVPLA
                     GFYTGYRRSVLAADELIEAIEIALPPEGSPFAWRKVGTRQAQAISKVAFAGLAVVASG
                     RLARLGLGMASVAPTVALLPATRALGLSKPLGEVTDADVEQAVLADVSPIDDVRSTAA
                     YRRHVAVRLAQRFFGELRAL"
     gene            complement(2676..3011)
                     /locus_tag="P5164_11390"
     CDS             complement(2676..3011)
                     /locus_tag="P5164_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HRY44537.1"
                     /translation="MFRTSVASVYPHYVTKAERNGRTKAEVDRIFCWLTGYSEKQLRQ
                     QLEKGTDFETFFAKAPALHPNSALITGVVCGVRVEEVEDPLMRKIRRLDKLIDELARG
                     RAMEKILRS"
     gene            complement(3156..3542)
                     /locus_tag="P5164_11395"
                     /pseudo
     CDS             complement(3156..3542)
                     /locus_tag="P5164_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008898980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
     gene            complement(3593..5593)
                     /locus_tag="P5164_11400"
     CDS             complement(3593..5593)
                     /locus_tag="P5164_11400"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY44538.1"
                     /translation="MARRRPRPEADRSAPLASPPRSFDSGRAAAAGAALVVALVAIWV
                     FLPVAGFGYVNFDDPNYLRDNAVVLEGLTAEGVAWAFETFHTGNWHPLAWLSHMADVE
                     LLGPEPGRHHAMGLALHVVSSLLLLFLVRGWTGSAGVAAAVAALFAWHPAHVESVAWL
                     SERKDVLSALFLWLALAAYTAYARRAAGARALPRLALVALCLALALLAKPMAVTAPFL
                     MLLLDAWPLGRLPLDEKPTWARLRPLLLEKLPLFALVALSSVVTFLAQSRGGGVSSLE
                     VLPLSERLGHAAVSVATYLRMLVWPAGLAVFYPHRGAPAPLELAGAILLLAALTFAAW
                     RARRSAPFLLVGWLWFVGTLVPVIGLVQVGEQSVADRYTYVPYVGLALALAEGARRVA
                     SRRAAAGVALAAVGGVALLALAPVARGQVGYWRDGATLFRRTLDVTGENHVAERLLGA
                     ALLSSGDAAGAEPHLREAVRLRPGSAEALNALGVALTQLGRPADAETALEAFRRAAIA
                     DPADAAIRNNLGNALAARGDAPGALAEFREAARLAPSEAAGHRNAGLTLAEMGRLEEA
                     RATFEEAVRLEPGDASTIGDLANVLAGLGRADEALARYREALRLDPSSRLVRHNLARL
                     LARTGRLDEATAVLAEGLARDPADEEGRLLLVRVRAVAGKTS"
     gene            complement(5660..6502)
                     /locus_tag="P5164_11405"
     CDS             complement(5660..6502)
                     /locus_tag="P5164_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44539.1"
                     /translation="MSDVEVRLPGGGLPGEIVNEAGDRVPNAVLAIVARGWREANTEL
                     IPDGTFLLEGLADGEYVVSAFLPRGGAESDGVLVRIEDGEVEEPVRLVLLPLHAIRGR
                     TVAAPGIGLPRASVERLPDPGPVPLRGSLPPAGLDGSWVAEVARTRDFACLIHGSPGR
                     ATRMFRMEPRPEQQEVLLTSVGGELVVNATKMPEGGPFPALFHNGCMGYQRALGRQGL
                     AAESEESGVVRVQGLFEPGEYSLCMITHEEHATYGGGRPAFPSCVHGTLLPGARLELT
                     APDR"
BASE COUNT         1049 a         2783 c         2563 g          940 t
ORIGIN      
        1 aggacgtcac cgtcgaggcg ctcgccgtcg cgacgaacac gccgccgaac ggcgccttcc
       61 gcggcttcgg cgcgccgcag acgatctggg cgatcgagcg tcacctagac cgggtcgcgc
      121 ggacgctcgg cacggacccg gtcgcgttga agaggaagaa cctcctccgc ctcggcgaca
      181 cgacggcgac gggccagacg ctgaaggagt cggtcggcgt cgaggagtgc gtcgagaagg
      241 caatggccgc gagcggctac gtcgagaagc gggaacgcgg ccccgtcgta acgggccgaa
      301 cggcgcgcgg cgtcggcgcc gcaatcttcc tccacggcgc cggcttcacc ggctcgggcg
      361 agaagtacct gaagggaaag gtcgccgtcg acctcctccc cggcggccgc gcccgcgtcc
      421 gcaccggctc gaccgacatc gggcagggga cggagaccgt cttccgccag atcgccgcgg
      481 acggcctcgg catcggcgtc gagaacgtcg acttcgccgt cccctcgacg gcgaacgtcc
      541 ccgactcggg gccgaccgtc gcctcgcgga ccgtcatggt cgtcggctcg atcgtcgacg
      601 ccgccgcgaa ggagcttcgc gcccgcctcg aggccgagga gaaggcccgg ggcgcgaccc
      661 gctccgccgc gtgggagcgc ctcgccgccg accccgcgcg cgcgacctcg ctcgtccagt
      721 acgaaccgcc gccgggcgtc gtctgggacg acgccaccta ctccggcgac gcctaccccg
      781 cctacggctg ggcctgcgac gtcgccgagg tcgaggtcga cctcgacacc ttcgagacga
      841 aggtgaccgg cttctgggcc gcggtcgacg tcggccgcgt catcaacccg acgctctgcg
      901 ccggccaggt cgaaggaggg accctccagg cgatcggctg ggcgctgacg gagaagctcg
      961 tgacgaagga cgggaagatc ctgaacccgc ggatgacgaa ctacatcgtc ccgacgagcc
     1021 tcgacgcgcc gccgtttacg acgacgctcg tcgaggcggc ctactcgcgg ggccccggcg
     1081 gggcgaaggg agtcggcgag ctgccgatgg acggcggcgc gccggccgtc gcggccgcca
     1141 tcgagcacgc cacggggctc gtcctcgacc gcgtcccgat gctccccgag gacctcttcg
     1201 aggcctccgt gaagggaggc gcgcgatgag catggagacg aatatcaccc tcaccgtcaa
     1261 cggctccgtc gtcacgctcg aggcaccccc cgccgcgcgc ctcctcgacg tcctccgcga
     1321 gaggctcggc ctcaccggga cgaaggaagg gtgcggcgag ggggagtgcg gcgcctgcac
     1381 cgtcctcctc gacggcgtcc ccgtgaacgc ctgcctcgtc gccttcggcc agtgcgacgg
     1441 gcgcgaggtg acgaccgtcg aggggctcgg cagcaccgcg cacttgacgc ccctccagga
     1501 ggcgatggtg acggaagggg gcgcgcagtg cggcatctgc acccccggca tgctcctctc
     1561 ggccgaggcc ctcctccgcg aggagccgcg gcccaccgag ccccagatcc gcgaggcgat
     1621 cgccggcaac atctgccgct gcaccggcta ccagaggatc gtgaacgccg tgaacgaggc
     1681 ggcgaggcgg cgggaggcgc gctcgtgagc gtcgccggcc tcgccctcca cccgttctcc
     1741 acgccgaaga cgctcgacga ggcgctcgcc ctcctcgccg agcgctccgc gagagccgag
     1801 aagaccgtcc tcctcgccgg gggcacggac tggttcgtcg agcggcacgt cgcgccggcc
     1861 gaagaagcgg gcgtgctccc cctcgtcgtc gacgtctccc gcctcgacgc cctccgcggc
     1921 atcgctgtcg aaggggaaac cctccacatc ggcgccgcgg cgacctacct cgagatccgg
     1981 aaggacgccc gcgtcgccgc ccggtgcccg ctccttgccg cgacggcccg cgacgtcggc
     2041 gcgctccaga tccaggcgcg cggcacgctc ggcggcaacc tcgcctccgg ctcccccgcc
     2101 gccgacggcg tcgccgccct cgccgcgctc gacgcgacac ttctcctccg gagcatccgc
     2161 ggcgcgcgcc gcgtcccgct cgccggcttc tacaccggct accgcaggag cgtcctcgcg
     2221 gccgacgagc tgatcgaggc gatcgagatc gcgctcccgc ccgagggctc ccccttcgcg
     2281 tggcggaagg tcggcacgcg ccaggctcag gcgatctcga aggtcgcctt cgccggcctc
     2341 gcggtcgtcg cttccggccg cctcgcgcgc ctcggcctcg gcatggcttc cgtcgccccg
     2401 acggtagcgc tcctcccggc gacccgcgcc ctcggcctct cgaagcccct cggcgaggtc
     2461 accgacgccg acgtcgagca ggccgtcctc gccgacgtct ccccgatcga cgacgtccgc
     2521 tcgacggcgg cctaccggcg ccacgtcgcc gtccgcctcg cgcagcggtt cttcggagag
     2581 ctgcgggcgc tctgaccttc acccgccccc gtgcgccggc tcccgcccgc gcacggggtc
     2641 gccggaggag aacggtcccg gcaaagccac ccgtcctacg accgcaggat cttctccatc
     2701 gcccggccgc gggcgagctc gtcgatgagc ttgtcgagcc ggcggatctt ccgcatcagc
     2761 gggtcctcga cctcttccac ccgaacgccg cagacgacgc ccgtgatgag cgcgctgttc
     2821 gggtggaggg ccggcgcctt cgcgaaaaag gtctcgaagt cggtcccctt ctcgagctgc
     2881 tgccgcagct gcttctcgct gtagccggtc agccagcaga agatccggtc gacctccgcc
     2941 ttcgtgcggc cgttccgctc cgccttcgtc acgtagtgcg ggtagacgct cgcgacgctc
     3001 gtccggaaga tgcggtgggc ggcggtcgtg cctccgccgg tcgccgcgcg gggcggggtt
     3061 gcgcgcttcg ccggcggacg cttctcgcct ccagggctcg gcttcgcagg cggcgccgtc
     3121 tccttcggct tcggcgaggc cgcggcggcc gtccccttca gccgcaccag ccggcccttc
     3181 cggcggacga tgttcttgta gtcccactgg acgtcccgcg cctgggcgag ccagcgccga
     3241 agcttctccg tgtcgacctc gccggcggac gtgtagcgcg cctcggccgc cttgaagctc
     3301 ccttccttcc ggagcccctt ctccgcgaac gactgcccgc tccagaagag gaggcggacg
     3361 ccgtccttca gcttgctgta gccgacgatc gggttcccgt cgaggaacca gaccgggtgg
     3421 gcatgccaga ccttgttctc cgcgtcgggc agcgcccggt cgatctcctt cgcgaggagc
     3481 tcgcacgtct ttcggtcggc gggcgcgagg gcggcgttgt acgtacgggt gtccgcgtgc
     3541 atcgagacct cctcgttcgg tcgggaatgg ggtctggtct acactctaca ctctacgagg
     3601 tcttccccgc gactgcccgg acccgcacga ggagaaggcg cccctcctcg tcggccgggt
     3661 cgcgcgcgag cccctcggcg aggacggccg tcgcctcgtc gagccgcccg gtccgcgcga
     3721 ggaggcgcgc gaggttgtgg cggacgagac gagacgaggg gtcgaggcgg agcgcctcgc
     3781 ggtaacgcgc gagcgcctcg tcggcacggc cgagcccggc gaggacgttc gcgaggtccc
     3841 cgatcgtcga cgcgtccccc ggctcgaggc ggaccgcctc ttcgaacgtt gcgcgcgctt
     3901 cctcgagccg ccccatctcg gcaagcgtca gtcctgcatt ccggtgcccc gccgcctcgc
     3961 tcggcgcgag ccgcgccgcc tcgcggaatt cggcgagcgc ccccggcgcg tcgccacggg
     4021 ccgcgagggc gttgccgagg ttgttgcgga tcgccgcgtc cgccggatcg gcaatcgccg
     4081 cccgccggaa cgcctcgagc gccgtctcgg cgtcggccgg ccgccccagc tgcgtcagcg
     4141 ccacgccgag cgcgttgagc gcctcggccg acccgggccg gagacggacg gcctcgcgca
     4201 ggtggggctc ggcccccgcc gcgtcgcccg aagagaggag cgcggcgccg agcagccgct
     4261 ctgcgacgtg gttctcgccg gtcacgtcga gcgtgcggcg aaagagcgtc gcgccgtcgc
     4321 gccagtaccc gacctgcccg cgggcgaccg gggcgagtgc cagcagcgca acgcctccca
     4381 ccgcggcgag cgcgaccccg gccgccgcgc gccgtgaggc cacgcggcgc gctccttccg
     4441 cgagcgcgag cgcgagtccg acgtacggga cgtacgtgta ccggtcggcc accgactgct
     4501 cccccacctg gacgaggccg atcaccggga cgagcgtccc gacgaaccag agccacccca
     4561 cgagaaggaa cggcgcgctc cggcgcgcgc gccacgccgc gaacgtgagg gccgcgagga
     4621 gaaggattgc cccggcgagc tccagcggcg ccggcgcgcc ccggtgcggg tagaagaccg
     4681 cgaggccggc cggccagacg agcatccgga ggtacgtggc aacagagacg gccgcgtgcc
     4741 cgaggcgctc ggagagcggg aggacctcca ggctcgagac gcctcctccg cgcgactgcg
     4801 cgaggaacgt cacgacgctc gagagggcga cgagcgcgaa gagcgggagc ttctcgagga
     4861 gaagcggccg gagccgagcc cacgtcggct tctcgtcgag cggcaggcgg ccgaggggcc
     4921 aggcgtcgag gaggagcatc aggaacgggg ccgtcacggc catcggcttg gccaacagcg
     4981 cgagggcgag gcagagcgcc acgagggcga gacgcgggag ggcgcgggcg cccgccgccc
     5041 gcctcgcata cgcggtgtac gccgcgagcg cgagccagag gaagagcgcg gagaggacgt
     5101 cctttcgctc cgagagccac gcgaccgact cgacgtgcgc cgggtgccag gcgaagagcg
     5161 cggcgaccgc cgccgcgacg cccgcgctcc ccgtccaccc gcggacgagg aagaggagca
     5221 gcagcgacga gacgacgtgc agggcgagtc ccatcgcgtg gtggcgcccc ggctcggggc
     5281 ccaagagctc cacgtcggcc atgtgggaga gccaggcgag cgggtgccag ttccccgtgt
     5341 ggaacgtctc gaaggcccac gccacgcctt ccgcagtcag cccctcgagg acgacggcgt
     5401 tgtcgcgcag gtagttcggg tcgtcgaagt tgacgtagcc gaacccggcc accgggagga
     5461 agacccagat cgcgaccagg gcgacgacca acgccgcgcc ggccgccgcc gcacggccgg
     5521 agtcgaacga acggggaggg gacgccagcg gagcggagcg gtcggcctcg gggcgaggcc
     5581 tgcggcgggc catggcctca ggatagcgga gaggctgcgt ggaggcgcgg cctggcgcgc
     5641 gcctccacgc agcgaacggt caacgatcgg gcgccgtcag ctccagccgc gcgccgggga
     5701 ggagcgtgcc gtgcacgcag ctcgggaacg ccgggcggcc gccgccatac gtggcgtgct
     5761 cttcgtgggt gatcatgcag agtgaatact cgcccggctc gaacagcccc tgcacgcgaa
     5821 caacgccgct ctcctccgac tcggcagcga gcccctgcct gccgagcgct cgttggtagc
     5881 ccatgcatcc gttgtggaag agcgccggga acgggccccc ctcaggcatc ttcgtcgcgt
     5941 tcacgacgag ctcgccgccg acgctcgtca ggaggacctc ctgctgctcc ggtcgcggct
     6001 ccatccgaaa catcctcgtg gcccgccccg gcgagccgtg aatcaggcac gcgaaatcgc
     6061 gggttcgggc gacttcggcg acccagctcc cgtcgaggcc tgcgggcgga agagagcccc
     6121 gcagcgggac cgggcccggg tccggcaagc gctcgacgct cgctctcggc agcccgattc
     6181 cgggggcggc cacggttcgc ccgcgaatcg catggagcgg cagcaggacg aggcgaaccg
     6241 gctcttcgac ctctccgtcc tcgatccgga cgagcacccc gtcactctcg gccccgccgc
     6301 gcgggaggaa cgcggaaacc acgtactcgc cgtcggcgag tccttcgagg aggaacgttc
     6361 cgtccgggat cagctccgtg tttgcctctc gccacccgcg cgcgacgatg gccaggaccg
     6421 cgttcggaac gcgatcgccg gcttcgttca cgatctcccc cggaagccct cctcccggca
     6481 gccgaacctc gacgtcggac acgccgtcgt ctcccggcgc gggaacgtcg acgtccgtcg
     6541 atcgcaggac ggttggtccg gacgaacgaa cggagaccga ccacgcgccg aggcggggga
     6601 gtttcccctc gaatcggccg gagacgtcgg actccatacg gatcgccggg aaccggcctt
     6661 caccgccaaa ggtgaccacc gcggcgatgg gccggcgccc gagcaggacc ttcccacgga
     6721 cttcctcgac gcggacctcg atcgatcgcc ggagatgcgc ggccgccatc tcgaatggtt
     6781 caacatcctt ccaccaaccc acacccaggc tcgtcttcag tctcactacg tatcttcggc
     6841 ccgtgaccat tcgcgcgtac gaccacgagc cgtcggctgc cagcggcgac tcgacgactc
     6901 gctccggcgc ggcgcccgtc cgcatctcga taacttcgac gatccaggtc gatcctgccg
     6961 ggtccagagg aggccggact cccagttcga gcttcccgta ctcgccaagg acgagcggat
     7021 cgttgagact cgcctcgaga ccgttcgcaa tctcgacgtc tcgcgtggct tccgcgagtc
     7081 ccggctgcga agcgacgacc ttccaccgcc cggcgtcgac tccctccagc tggaagaacc
     7141 cacgggccgt cggacggacg gttcggctcg tacggaccgc gccgcccgca cccgttccgc
     7201 gcgaatcgcc ggcctcgggc ctcagcgtga tcagacaccg atccgggtcg agacgtccgg
     7261 ctccggcgac ctcgaccgac ccgacgagcg aggcgccggg acgcagctcg atcgctccga
     7321 ggtttctcgg cggcc
//
LOCUS       DAOTEZ010000201         7328 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_7209_length_7328_cov_6.588203, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000201 DAOTEZ010000000
VERSION     DAOTEZ010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7328
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7209_length_7328_cov_6.588203"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="P5164_11410"
     CDS             complement(<1..638)
                     /locus_tag="P5164_11410"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRY44540.1"
                     /translation="MSERTVVVTGLGAVSSLGNDLASTWAGLVAGRSGIGPITLFDAS
                     GFRTKLAAEVPQLPDCGVPSRLRRRLSRTDVIGLAAAREALADSGLDLDLDREDPGRI
                     GVVLGGGVSGLLDSEGYFRDLLAKGPRGARPTRILNHPPDQTTDRIAEVWGFRGPRAT
                     ITTACSSSSTSLGYAADLIRAGMADVVVTGGADSFARLTHGGFNALRACDPEP"
     gene            706..1140
                     /locus_tag="P5164_11415"
     CDS             706..1140
                     /locus_tag="P5164_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HRY44541.1"
                     /translation="MTERVDWNRYFMNIAAEVATRSTCPRKHVGAVIVRDRRILSTGY
                     NGSLRGLPHCDESGCLMEDGHCIATVHAEANAILQAAMNGVRIEGGEIYTTASPCWPC
                     FKLIGNAGLVGIYFSEFYREPRILDYAERMGIRLVDLGPGRR"
     gene            complement(1144..1818)
                     /locus_tag="P5164_11420"
     CDS             complement(1144..1818)
                     /locus_tag="P5164_11420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY44542.1"
                     /translation="MHTILLVDDMRSFLDLEATFLSRAECRILTASTGLEAIRVARTE
                     KPDLIVLDIEMPEMNGIQACRILKSDPVTAHILVVMLSSMKLEDEGRRAGADHFLRKP
                     IDEPTFLSEVKRFLPIVERAETRVAVDSPITFWRDGETFEGRLTNLSRTGFYVAVSRI
                     HPIGARLEVSFALPNDPEGKVVTGEALVVRHDDDPPGFGCRFRQVSSGARLVVEDWVE
                     RVAAGR"
     gene            complement(1860..3734)
                     /locus_tag="P5164_11425"
     CDS             complement(1860..3734)
                     /locus_tag="P5164_11425"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRY44543.1"
                     /translation="MLRTVALALVLFASAPSLAVRPFEEEDAPAPVKAEVAVVASHAG
                     AGAEAATVAVLGREEIERLPARSLADLLRYLPAVDVRRRGPGVQADVSLRGADYNGTL
                     ILVDGEPVNDPQTNHHSLDLDVPADAIERIEVLYGSASALWGSEAVGGVIHVITRGAS
                     LGRARAQIEGRYVHGSHSLDAGSVRVATRLSDSLTVSVDASRSESSGFRDDREHAEKT
                     VRASLRLETRAGPVTLSGGGASRAFGAWGFYGTRYPNQYEETRSRSLRLAADLDLGRG
                     WTLAPSVSARDHRDDFVLERTDPSFYRNLHDTDRSAVRLVARRPIFGGSLALGGETGR
                     DAIDSTSLGRRTRTRSAAFVELARPFAAASPSAGGVRAGLRADRHEGFGTRLSPQLAA
                     WVGLGAGVKARASVGTAFRVPTFTELHYVDPQTVGNPALKPEKSTSFEAGLSWDHGAL
                     SFDAAVFHRHGTDLVDFVRTGPDEPYRATNLRTVDTRGLEATLVLDAARLAPTPLARL
                     AFRAAVYSADLERLRAEAGATEGRYVLDPLRTRWDLLAEVRVPFRVEALARLSYFDRP
                     SFDEGVLLLDARLGYDVVDGDVVELYVEGENLGAVRYEEVPGVPLPGRTLAAGVRLTW
                     "
     gene            3782..4357
                     /locus_tag="P5164_11430"
     CDS             3782..4357
                     /locus_tag="P5164_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44544.1"
                     /translation="MNASRAVVLALAGALLAPPASAEKPKPRAVLVEADGDEEALSAF
                     VARLESELSDRGKVTLSDARLAGISLGAVVAEPDGEAARVLRAEWAGTSWIGVSLAPC
                     RVDVSRMTYRETSPEGYAYDRVVESVRVDCPATLRIVDAATGKEKKPLEVTGTANYRR
                     SGPDDEESPQLEATRDAAEKAAKKLPAALGR"
     gene            4476..5405
                     /locus_tag="P5164_11435"
     CDS             4476..5405
                     /locus_tag="P5164_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44545.1"
                     /translation="MKHLRRPILPALAILLAPAVALPLDVVNLPEGTGAAGIAVARDG
                     TVWFSAQFSGALGRVSPSGEVSLFPFPFRDTQPLSIAVQPDGTAWGADLAGERLGRVA
                     PDGTFTEISTGRAGGEPTGLAVSAGGVAWFTRPAEGRVGHRGSDGTVRELVLPEASEP
                     TAIVVARDGAAWVVERRGNRLVRISTDGEITAWPLATPNAQPDALAVAPDGSVWAALP
                     NARRIARVSPRGTLEEISVDNAPAGIAVAPDGGVWFAAPTAKKLGRLEPETGKVEWTP
                     LPDGPVAVSAAADGAVWVVLGIGDKLGRIEPKR"
     gene            5571..6149
                     /locus_tag="P5164_11440"
     CDS             5571..6149
                     /locus_tag="P5164_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRY44546.1"
                     /translation="MDAAILLFTNRGYAATSVREIVEMAGVTKPSLYYHFQSKEGIYL
                     AIIEDLVKIADESISATRVSVGTARERLQAFLLGIFRVFEMKKAEVRMMNAVFWGPAQ
                     GAPPFDFEVFHEKLKAVIAEIVAEGISAGDLRAADPQDVTLALMGVLSFNMDLTLAHP
                     ELGLGQDGLRRTLDLVFEGLAAPRNAQQEPAR"
     gene            6146..7252
                     /locus_tag="P5164_11445"
     CDS             6146..7252
                     /locus_tag="P5164_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY44547.1"
                     /translation="MNRTTLAAPSAALLLVLAAGAAGCGKPAAGDGQRPPVAVETATT
                     AASDLEESVEVVGTLAARSEVEVKTEYSGTVAEVFVTQWVRVAAGTPLARLDSREAEA
                     GVQAAKATWLQAEAGAKRAARELDRTVKLKEAGLATQQQLDEARSAEEAARAAAEAAK
                     AQHALAETRLAKAVLRAPIDGVVAVRNVNVGDYVENMGNPAPMFRIVDNRVLELTASV
                     PSARMAALRVGQSFAFTSDALAGRTFSGRVSFINPAADEASRTVKVKAEVPNGDGALR
                     AGLFVKGRIVTGRRPGVLVVPRSALVSWDTVARTAGVFVVEGGVAKRLSVETGAAPGD
                     VVEVVKGLTAGQDVVTRGGFNLREGDRVLVSKGA"
BASE COUNT         1028 a         2564 c         2763 g          973 t
ORIGIN      
        1 ggctcggggt cgcaggcgcg gagggcgttg aagccgccgt gcgtgaggcg ggcgaagctg
       61 tcggcgccgc ccgtgacgac gacgtcggcc attcccgcgc ggatcaggtc cgcggcgtat
      121 ccgagcgacg tcgacgagga ggagcaggcc gtcgtgatcg tcgcgcgcgg gccgcggaag
      181 ccccagacct cggcgatccg gtcggtcgtc tggtcggggg gatggttcag gatccgggtc
      241 gggcgggccc cgcgcggccc cttcgcgagg aggtcgcgga agtacccctc gctgtcgaga
      301 aggcccgaga cgcctccccc gaggacgacg ccgatccggc cggggtcctc ccggtcgagg
      361 tcgaggtcga ggccggagtc ggcgagcgcc tcgcgcgccg cggcgaggcc gatgacgtcg
      421 gtccgggaga ggcggcggcg gagccgggac gggacgccgc agtccgggag ttgcggcacc
      481 tcggcggcga gcttcgtccg gaagccgctc gcgtcgaaca gggtgatcgg gccgattccg
      541 ctccggccgg cgacgaggcc ggcccaggtg gaggcgaggt cgttcccgag ggagctgacg
      601 gccccgaggc cggtgacgac gacggtccgc tcggacatgc cgggaatcgt acacggggac
      661 gccgcggcgc agcaggagga cgcgcccgca cggataatcg ggcgcatgac cgagcgcgtc
      721 gactggaacc ggtacttcat gaacatcgcg gcggaggtgg cgacccgctc gacctgcccg
      781 cgcaagcacg tcggcgccgt catcgtccgg gaccggcgga tcctctccac cggctacaac
      841 ggctctctcc ggggcctccc gcactgcgac gagtcgggct gcctgatgga ggacgggcac
      901 tgcatcgcga ccgtccacgc cgaggcgaac gcgatcctcc aggcggcgat gaacggcgtc
      961 cggatcgagg ggggcgagat ctacacgacc gcctctccct gctggccctg cttcaagctg
     1021 atcgggaacg ccggcctcgt cgggatctac ttctcggagt tctaccggga gccgcggatc
     1081 ctggactacg ccgagaggat ggggatccgc ctcgtcgacc tcggccccgg gcggcgctga
     1141 gcctcagcgc ccggcggcga cgcgctcgac ccagtcttcg acgacgaggc gggcgcccga
     1201 ggagacctgg cggaaccggc agccgaaccc gggcgggtcg tcgtcgtggc ggacgacgag
     1261 cgcctccccc gtgacgacct tcccctccgg gtcgttcggg agcgcgaacg acacctcgag
     1321 ccgagccccg atcgggtgga ttcgcgagac ggccacgtag aagccggtcc gcgagaggtt
     1381 cgtcaggcgt ccctcgaacg tctctccgtc gcgccagaac gtgatcggcg aatcgaccgc
     1441 gacgcgcgtc tcggcccgct cgacgatcgg caggaagcgc ttgacctcgg agaggaacgt
     1501 cggctcgtcg atcggcttgc ggaggaaatg gtcggcgccc gcgcggcgtc cctcgtcctc
     1561 cagcttcatc gaggagagca tcacgacgag gatgtgcgcg gtgacgggat cggatttcag
     1621 gatgcggcac gcctggatgc cgttcatctc cggcatctcg atgtcgagga cgatgaggtc
     1681 gggcttctcc gtccgggcga ctcggatcgc ctcgaggccg gtcgaggcgg tcaatatccg
     1741 gcactcggcg cgcgagagga acgtggcctc gaggtcgagg aagctcctca tgtcgtcgac
     1801 gagcagaatc gtgtgcatcg aaccggtacg ccctcgcttt cccgagttta cgctccgcct
     1861 caccaggtga ggcgcacccc ggcggccagg gtccggcccg ggagcggcac gcccggcacc
     1921 tcctcgtagc ggacggcccc gaggttctcc ccctcgacgt agagctcgac gacgtcgccg
     1981 tccacgacgt cgtacccgag gcgggcgtcg aggaggagga ccccttcgtc gaacgagggg
     2041 cggtcgaagt acgagaggcg cgcgagcgcc tcgacgcgga aggggacgcg gacctccgcg
     2101 agcaggtccc agcgcgtccg cagcgggtcc aggacgtagc gtccctcggt cgcgcccgcc
     2161 tcggcccgga gcctctcgag gtcggccgag tagacggcgg cccggaacgc gaggcgggcg
     2221 aggggcgtcg gggcgaggcg cgccgcgtcg aggacgagcg tcgcctcgag gccgcgcgtg
     2281 tcgacggtcc ggaggttcgt ggcccggtac ggctcgtccg ggccggtacg gacgaagtcg
     2341 acgaggtcgg tgccgtgacg gtggaagacg gccgcgtcga acgagagggc gccgtggtcc
     2401 caggagaggc ccgcctcgaa cgaggtcgac ttctcgggct tcagcgcggg gttgccgacg
     2461 gtctgcgggt cgacgtagtg gagctcggtg aacgtcggga cccggaaggc ggtcccgacc
     2521 gaggcgcggg cctttacgcc ggccccgaga ccgacccacg ccgcgagctg cggtgagagg
     2581 cgcgtcccga aaccttcgtg ccggtcggcg cggaggccgg cgcggactcc tccggcggac
     2641 ggggacgccg cggcgaacgg ccgggccagc tcgacgaacg cggcgctccg ggtgcgggtg
     2701 cggcgaccca ggctcgtcga gtcgatcgcg tcgcgccccg tttcgccccc gagggcgagg
     2761 gagccgccga agatcgggcg gcgcgcgacg aggcggacgg ccgagcggtc ggtgtcgtgg
     2821 aggttccggt agaaggaggg gtccgtccgc tcgaggacga agtcgtcccg gtggtcgcgc
     2881 gcggagaccg agggggcgag ggtccagccg cggccgaggt cgaggtcggc cgcgaggcgc
     2941 agactccggc tcctcgtctc ctcgtactgg ttcgggtagc gcgtcccgta gaagccccag
     3001 gctccgaagg cccggctcgc gccccctccc gagagcgtga ccgggccggc ccgggtctcg
     3061 agccggagcg aggcgcggac cgtcttctcg gcgtgctccc ggtcgtcgcg gaagccgctc
     3121 gactcggagc gcgaggcgtc gaccgagacg gtcagggaat cggagagcct cgtggcgacc
     3181 cggacgctcc cggcgtcgag cgagtgggag ccgtggacgt accggccctc gatctgggcg
     3241 cgcgcccggc cgaggctcgc cccgcgcgtg atgacgtgga tcacgccccc gacggcctcg
     3301 gagccccaga gcgccgaggc ggagccgtag aggacctcga tccgctcgat ggcgtcggcc
     3361 gggacgtcga ggtccagcga gtggtggttc gtctgggggt cgttgaccgg ctccccgtcc
     3421 acgaggatca gcgtgccgtt gtagtcggct ccgcggaggg agacgtcggc ctggaccccg
     3481 ggtccgcgac gcctcacgtc gacggcaggg agatatcgga ggaggtcggc cagcgagcgt
     3541 gcgggcagcc gctcgatctc ctcccggccg aggacggcga ccgtcgccgc ctccgctccc
     3601 gcccccgcgt gcgaggcgac gacggccacc tccgccttca cgggggcggg tgcgtcctcc
     3661 tcctcgaacg gccgcaccgc gagcgacggg gcggaggcga agaggacgag ggcgagcgcg
     3721 accgtgcgga gcatgcggcg cggaggatag cggacgcggc ccgtcgcgct actcttccgg
     3781 catgaacgcc tctcgcgccg tcgtcctcgc gctcgccggg gccctcctcg cgccccccgc
     3841 atccgccgag aagccgaagc cgcgagccgt cctcgtggag gccgacggcg acgaagaggc
     3901 gctctccgcg ttcgtggccc gtctcgagtc ggagctctcc gatcgaggga aggtgacgct
     3961 ttcggacgcc cgactcgccg ggatctccct gggagccgtc gttgccgagc cggacggcga
     4021 ggccgcccgc gtcctgcggg cggaatgggc cggaacgagc tggatcggcg tctcgctcgc
     4081 cccctgtcgc gtcgacgtga gccggatgac ctaccgcgag acgagcccgg aggggtacgc
     4141 gtacgaccgg gtcgtggaga gcgtccgggt cgactgcccg gcgacgctcc ggatcgtcga
     4201 cgccgccacc ggcaaggaga agaagcctct ggaggtgacc ggaacggcca actaccggag
     4261 gagcggtccc gacgacgagg agagcccgca gctcgaggcg acccgcgacg ccgccgagaa
     4321 ggccgcgaag aagctcccgg ccgcgctcgg ccgctgaacg cgccaccgga aggcggcccc
     4381 gccggggcaa cggggatact tcgtgcggac gtcgtgccct gggcctatcc tcggctgttc
     4441 ggacgcccgg catgcggcgt ccgtggcggt tcgacatgaa gcacctccga cgcccgattc
     4501 ttcccgcgct cgcgatcctc ctcgcaccgg ccgtcgccct ccctctcgac gtcgtgaacc
     4561 tcccggaggg gacgggagcg gcggggatcg ccgtcgcccg cgacgggacg gtctggttct
     4621 cggcgcagtt ctccggagcg ctgggccgcg tgagcccgtc cggcgaggtg tcgctcttcc
     4681 cgttcccctt tcgcgacacc cagccgctct cgatcgccgt ccagccggac ggcacggcgt
     4741 ggggcgccga cctcgccggg gagcggctcg gccgtgtcgc gccggacggc accttcacgg
     4801 agatctccac cgggagagcg ggaggggagc cgacggggct cgccgtatcc gcaggcgggg
     4861 tcgcctggtt cacgcggccg gccgaggggc gcgtcgggca ccgcggctcg gacgggacgg
     4921 tgcgcgagct cgttcttccg gaggcgagcg agccgacggc aatcgtcgtc gcccgcgacg
     4981 gagccgcgtg ggtcgtcgag cgaaggggaa accgcctcgt gcgcatttcg acggacggcg
     5041 agatcacggc ctggcctctc gcgacgccga acgctcagcc cgacgctctt gccgtggcgc
     5101 cggacgggtc cgtctgggca gccctgccga acgcgaggcg catcgcccgc gtctcgccgc
     5161 ggggcaccct cgaggagatc tccgtcgaca acgccccggc cggcatagcc gtcgcgccgg
     5221 acggaggggt ctggttcgcg gccccgacgg cgaaaaagct cggccggctc gagccggaga
     5281 cgggcaaggt cgagtggacg ccgcttccgg acgggccggt cgccgtctcg gcggctgcgg
     5341 acggagctgt ctgggtcgtc ctcggcatcg gcgacaagct cgggcggatc gaaccgaagc
     5401 gctagacgcg cccaggggag cttttttttc gtcctgccct tgacgggcgc ctccctcggc
     5461 cccagattac ctaccggtcg gtaacccgag ccggaggacg gtgacgggtt gccggggagg
     5521 ttcgatccgt gccgcaggaa cctggtgaaa gctctgtccg aaagagactt atggatgccg
     5581 cgatcctcct cttcacgaat cgcggctatg ccgcgacctc tgtgagggag atcgtggaga
     5641 tggccggcgt gacgaagccg tcgctctact accacttcca gagcaaggaa gggatctacc
     5701 tcgcgatcat cgaggacctc gtgaagatcg cggacgagtc gatctccgcg acccgggttt
     5761 ccgtcggcac ggcccgcgag aggctccagg ccttcctgct cggcatcttc cgggtcttcg
     5821 agatgaagaa ggccgaggtc cggatgatga acgcggtctt ctgggggccg gcccaggggg
     5881 cgccgccgtt cgacttcgag gtcttccacg agaagctgaa ggccgtcatc gccgagatcg
     5941 tcgcggaggg catctccgcg ggcgacctcc gcgcggccga cccgcaggac gtcaccctcg
     6001 ccctgatggg cgtcctctct ttcaacatgg acctcaccct cgcgcacccg gagctcgggc
     6061 tcgggcagga cggcctccgc cggacgctcg acctcgtctt cgagggcctc gccgcccccc
     6121 gcaacgcaca gcaggagccc gctcgatgaa ccgcacgact ctcgccgccc catccgcagc
     6181 ccttctcctc gtcctcgccg ccggcgccgc cggctgcggc aagccggccg ccggggacgg
     6241 ccagcgtccg cccgtcgccg tcgagacggc gacgaccgcg gcctccgacc tcgaagagtc
     6301 cgtcgaggtg gtcgggaccc tcgccgcccg aagcgaggtc gaggtgaaga ccgagtattc
     6361 cggcacggtc gccgaggtct tcgtgacgca gtgggttcgg gtcgcggcgg ggacgcccct
     6421 ggcccgcctg gactcgcgcg aagcggaggc cggggtgcag gccgcgaagg cgacctggct
     6481 ccaggccgaa gccggagcga agcgcgccgc gcgcgagctc gaccggacgg tgaagctgaa
     6541 ggaggccggc ctcgcgaccc agcagcagct cgacgaggcc cgctcggccg aggaggcggc
     6601 gcgcgccgca gccgaggccg ctaaggcgca gcacgccctg gccgagacgc ggctcgcgaa
     6661 ggccgtcctc cgcgcgccga tcgacggcgt ggtcgcggtc cggaacgtga acgtcggcga
     6721 ctacgtcgag aacatgggga acccggcgcc gatgttccgc atcgtcgaca accgggtcct
     6781 ggagctgacg gcgagcgtcc cgtcggcgcg gatggcggcg ctccgggtcg gacagtcgtt
     6841 cgcgttcacg agcgacgccc tcgcggggcg gacgttctcc ggcagggtct cgttcatcaa
     6901 cccggcggcc gacgaggcga gccggacggt gaaggtgaag gccgaggtgc cgaacggcga
     6961 cggggcgctc cgggcgggcc tcttcgtcaa ggggcgcatc gtcaccggcc gcaggcccgg
     7021 cgtcctcgtc gtccctcgct ccgcgctcgt ctcctgggac accgtggcgc gcacggccgg
     7081 cgtcttcgtc gtcgaggggg gcgtcgcgaa gcgcctctcc gtcgagacgg gcgcggcgcc
     7141 cggcgacgtc gtcgaggtcg tcaagggcct gaccgccggc caggacgtcg tcacgcgcgg
     7201 cggcttcaac ctccgcgagg gggaccgggt cctggtctcg aagggagcct gagccgtgtt
     7261 cctctccgac ctctcgatca agaggcccgt cttcgcgacg gtcctcatgc tcgccctcgt
     7321 cacgctcg
//
LOCUS       DAOTEZ010000202         7285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_7267_length_7285_cov_7.013416, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000202 DAOTEZ010000000
VERSION     DAOTEZ010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7285
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7267_length_7285_cov_7.013416"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..2207
                     /locus_tag="P5164_11450"
     CDS             162..2207
                     /locus_tag="P5164_11450"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRY44548.1"
                     /translation="MSRTAGPVAALSCGASPVLLRHAVRALAVLIPFLAVSGLPAEEP
                     PPTRAVELRVVDRLGRPVTDVSACLLPACTKVAWRTEKEGYIAGVPAGDAPAALRLTA
                     RSFENADLVVAPEAAATKATLKAKGSVRVVFLAPEGKETVRLTVSLKETIDPNAGAKG
                     RLLAERAVDVPPRPATSAVVLDDVPPGDWVLGWEGTSIAAGTKVVKVTETRSDAGSVG
                     LVRGWSISGSVRDDLGVVVPGARVRLRAGSPTTGRPVGTERVARTGSDGGFVVHGLPL
                     DEVLSWDSGTDEHEVSRGELGGETRLEIVLARAQRVFGRLVDEEGRPAAGVEIEVSYV
                     TRKRSTDSEGNERRFTMVEGHPGPVVAGEDGAFSFFRRLPASVQVAPQGKDRLPETRT
                     LEPLPADAVRGELDLGDLVLRAGRTLTGRVAKADGGAPVAGAELEASWTGEEAGALGS
                     ARGLSGADGSFRIEAIVPGREVTLTARKKGFSPRTLAVAPEADSVDVLLSRGGRVKGR
                     VCGTAWELASAAVWYGPDGAISNRSQAVVDSGGRFTLENAETGTLTFARSWRFRNPAN
                     PGSTWEWSGAVRANVEVKEGETAEVSLGCDGIPVSGLLLRDGRPVSSEIVSFSSGAGA
                     PATDAVTDASGRFFARVPSPRLWLLSAGGSPLSAAAACEVPPGGLEGCRVEIGKDGQ"
     gene            complement(2239..3243)
                     /locus_tag="P5164_11455"
     CDS             complement(2239..3243)
                     /locus_tag="P5164_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013575951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRY44549.1"
                     /translation="MKTLLTGAAGFIALHVAERLLDAGNEVVGVDNLNAYYDVSLKEA
                     RLARLLPREGFRLERLDLADRDGIAALFARERFEKVVHLGAQPGVRHSLDAPFDYVES
                     NLTGHLTVLEGCRRTGVAHLVYASSSSVYGLNATPFRASDRTDHPVSLYGATKKANEV
                     MSHAYAHLFGVPSTGLRFFTVYGPWGRPDMATMKFTRAILAGEPIEVYGHGEMKRDFT
                     YVGDIADSVVALLDRAPEADPAWTPEAGDPASSSAPHRVFNIGNGEPVLLLEFIRTLE
                     ELLGRKANLVLREMQPGDLVETRSDTEPLARLLGAAPYTPLAVGLARFVAWYREYYRG
                     "
     gene            complement(3240..4949)
                     /locus_tag="P5164_11460"
     CDS             complement(3240..4949)
                     /locus_tag="P5164_11460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005036726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY44550.1"
                     /translation="MTPDEALLEGPRDDRRALLLLLLLATVLFAAGLGLRDPWPADEP
                     RFALVARQMVESGQWLFPVRGGELYPDKPPLFMWAIALFLKLTGSLRVAFLLPSLLAG
                     LGTLALVFDLARRLHDRATAWRAGLLLLLTVQFTLQARTAQIDALVTLWITLGVYAFV
                     RFLLLGGGWRWYYLGWFAAGLGIITKGVGILALFALVPALSTHRAEIRRAPLSAWLKG
                     LAGPLALLAAVALWGVPMLVAVAQSGDPALEAYRNNILFKQTVTRYGAAWHHVEPWWD
                     YLVKVIPPFWLPLSLLLPWLVPAWVREVRAGDRRVPVLLGYLLLVLVFFSASPGKRGV
                     YITPATPALALLAAPLLPELLRKAWPRRLFTGLAAFVAAVGLLAALAALLVPRVGQKL
                     AEQEIANPWPAALVLGIAATALVLRFRHFALAAVTSVLAVVWLVVGFGVNPLLNGSRT
                     PKSVVEAAEARVPPGDALALVAYREQFLLFARRPTHHFPYLMPPEMQGAAAARWQAGE
                     PGRWVLGPDKALAESFDLSSAADLGKRHGRQWLLVPPGAARPAADAPDGPVYLYTPPK
                     TAP"
     gene            complement(4946..5248)
                     /locus_tag="P5164_11465"
     CDS             complement(4946..5248)
                     /locus_tag="P5164_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HRY44551.1"
                     /translation="MTVAVERIWVGLGLFGQLLFSARFIIQWIASERQKRSVIPVAFW
                     YFSLGGSALLLAYAIYRKDPVFILGQSAGAVVYVRNLWLIKAARHLEAGTDPGDGA"
     gene            complement(5245..5988)
                     /locus_tag="P5164_11470"
     CDS             complement(5245..5988)
                     /locus_tag="P5164_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010673218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY44552.1"
                     /translation="MPEQPFLSVVIPARNEAENVRPLVAEVRQALDGVFDYELIYVDD
                     GSTDATGETLKALAAEGFDRLRILRHEKSTGQSFAVLTGARAARGEWLVVLDADGQND
                     PADIPGMVRTALEGNARDPRVVGVIGHRVNRRDDWIKRVSSKVANGFRDAMLKDGVPD
                     VGCGLKSVRLDWYLRLPAFDHMHRYVPTLVQSLGGVMLVHPVNHRPRMAGRSNYGVWN
                     RLWVGLVDVLGVAWLKRRAKSPLVKEVLP"
     gene            6295..>7285
                     /gene="aceA"
                     /locus_tag="P5164_11475"
     CDS             6295..>7285
                     /gene="aceA"
                     /locus_tag="P5164_11475"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003186515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HRY44553.1"
                     /translation="MYRSQSIPELERSWANDLRWRGIVRPYSAADVDRLRGTIQIDYP
                     VARMGAERLWNLLHTETFVRALGALTGNQAVQQVQAGLKAIYMSGWQVAGDANDALTM
                     YPDQSLYPVTSVPTVIRRITNALMRADQIQHMEGRSDVYYFAPIVADAEAGFGGPLNT
                     FELIKSMIEAGAGGIHLEDQLSSLKKCGHMGGKVLVPIHDFISKLIAARLAADVLGVP
                     TVFIGRTDSLGASLIRGDIDKVDQEFLTGERTSDGFYIVKGSLEYAVARACAYAPYVD
                     VVWCETSTPDIGEARAFAQGVHEKFPGKLLAYNCSPSFNWKKKLSDSEIAAFQE"
BASE COUNT         1064 a         2522 c         2748 g          951 t
ORIGIN      
        1 gctcctcggc caggtcgtcg agcactcgcg gacggaagac ctcttcatca gcccggcgga
       61 cccccggacc gcggagtaca tcgaagggcg ctacgcctga cgccgcggcc gccggcgcga
      121 aggccgctcc ttccgcgagc ggacggtaca cttccgaggc catgtcccgc accgcaggcc
      181 cggtcgcggc gctctcctgt ggcgcgtcgc ccgtcctcct ccgacacgcc gtccgcgcgc
      241 tcgccgtcct gatcccgttc ctcgccgtct ccggcctgcc ggccgaggag cctcctccga
      301 cccgggccgt cgagctccgc gtcgtcgacc ggctcgggcg gcccgtgacg gacgtttcgg
      361 cgtgcctcct ccccgcctgc acgaaggtcg cgtggcggac ggagaaggag ggatacatcg
      421 ccggggtgcc ggcgggagat gctcccgcgg cgctccgcct caccgcgcgg tcgttcgaga
      481 acgccgacct cgtcgtcgcg ccggaagcgg cggcgacgaa agcgacgctg aaggcgaagg
      541 gctcggtgag ggtggtgttc ctcgctcccg aagggaagga gaccgtgcgg ctgacggtct
      601 ctctgaagga gacgatcgac ccgaacgccg gagcgaaggg ccgcctcctc gccgaaaggg
      661 ccgtcgacgt cccgccgcgc cccgcgacga gcgccgtcgt cctcgacgac gtcccgccgg
      721 gagactgggt cctcggctgg gaggggacgt cgatcgcggc cgggacgaag gtcgtgaagg
      781 tgaccgagac gcggtcagat gccggctccg tcgggctcgt tcgcggctgg tcgatctcgg
      841 gcagcgtccg ggacgacctc ggggtcgtcg ttcccggcgc gcgggtccgg ctccgggcgg
      901 gcagccccac gaccggccgg ccggtcggca cggagcgcgt cgcgcgaacc gggagcgacg
      961 ggggcttcgt tgttcacggc ctccccctcg acgaggtcct ctcgtgggac tcgggcaccg
     1021 acgaacacga ggtctcgcgc ggcgagctcg gcggcgagac gcggctcgag atcgtcctgg
     1081 cgcgcgccca gcgcgtcttc ggccgcctcg tcgacgagga gggacgtccc gccgccggcg
     1141 tcgagatcga ggtttcgtac gtcacccgga agcggtcgac ggactccgag gggaacgagc
     1201 ggcgtttcac tatggtcgag gggcaccccg gccccgtcgt cgcaggcgag gacggagcct
     1261 tctcgttctt ccgccgcctt ccggcttccg tccaggtggc gccgcagggg aaggaccgcc
     1321 tgcccgagac gcggacgctc gagccgctgc ccgccgatgc ggtccgcggc gagctggatc
     1381 tcggcgacct cgtcctccgc gcgggccgga cgctcacggg acgcgtggcg aaggccgacg
     1441 gcggcgcgcc cgtcgcgggc gcggagctcg aggcgagctg gaccggcgag gaggccggag
     1501 ccctcggctc cgcgcgcggc ctctcggggg cggacggctc gttccggatc gaggccatcg
     1561 tccccgggcg cgaggtgacg ctcacggcgc ggaagaaggg cttctcgccg cggacgctcg
     1621 cggtcgcccc ggaggccgac tcggtcgacg tcctcctctc ccgcgggggg cgggtgaagg
     1681 ggcgggtctg cgggacggcc tgggagctcg cctccgcggc cgtctggtac ggacccgacg
     1741 gcgcgatctc gaaccggagc caggccgtcg tcgactccgg gggtcggttc acgctggaga
     1801 acgccgagac gggcacgctc accttcgcgc ggagctggcg cttccggaac ccggcgaatc
     1861 cgggctccac ctgggagtgg agcggcgccg tccgcgcgaa cgtcgaggtg aaggagggcg
     1921 agacggccga ggtctccctc ggctgcgacg ggatccccgt gtccggcctt ctccttcgcg
     1981 acgggcgccc cgtctcctcg gagatcgtca gcttctcgag cggggccgga gcgcccgcga
     2041 cggacgcggt caccgacgcc tccggacgtt tcttcgcgcg cgtcccgtcc ccccgcctct
     2101 ggctcctctc ggccggcggc agcccgcttt cggccgcggc ggcatgcgag gtgccgccgg
     2161 gcggcctcga gggttgccgc gtggagatcg ggaaggacgg acagtgagcg gggcctgcgc
     2221 gcgccgcgcc tgccgccctc agccccggta gtactcccgg taccaggcga cgaagcgggc
     2281 gaggccgacg gcgagcggcg tgtagggggc cgcgccgagg aggcgcgcga gcggctcggt
     2341 gtcggacctc gtctcgacga ggtcgcccgg ctgcatctcg cgaaggacga ggttcgcctt
     2401 ccggccgagg agctcctcga gcgtccggat gaactcgaga aggaggaccg gctcgccgtt
     2461 gccgatgttg aagacgcggt gcggggcgga gctcgacgcc ggatcgccgg cctcgggcgt
     2521 ccacgcggga tcggcctcgg gcgcgcggtc gaggagggcg acgacggagt cggcgatgtc
     2581 gccgacgtag gtgaagtcgc gcttcatctc gccgtgcccg tagacctcga tcggctcgcc
     2641 cgcgaggatc gctcgcgtga acttcatcgt cgccatgtcg gggcggcccc acgggccgta
     2701 gacggtgaag aaccggaggc ccgtcgaggg cacgccgaag aggtgcgcgt aggcgtggct
     2761 catgacctcg ttcgccttct tcgtcgcgcc gtagaggctc acggggtggt ccgtccggtc
     2821 ggaggcgcgg aacggcgtcg cgttcaggcc gtagaccgag ctggagctcg cgtagacgag
     2881 gtgcgcgaca cccgtcctcc gacacccttc gaggaccgtc aggtgcccgg tgaggttcga
     2941 ctcgacgtag tcgaacggcg cgtcgagcga atggcggacg cccggctgcg cgccgaggtg
     3001 gacgaccttc tcgaaccgct cgcgcgcgaa gagggccgcg atcccgtccc ggtcggcgag
     3061 gtcgagccgc tcgaggcgga agccctcgcg cgggaggagc cgcgcgaggc gcgcctcctt
     3121 cagcgagacg tcgtagtacg cgttcaggtt gtcgacgccg acgacctcgt ttccggcgtc
     3181 gaggagacgc tcggcgacgt gcagggcgat gaagccggcg gcgccggtga ggagggtctt
     3241 cacggtgcgg tcttcggagg agtgtagagg tagacggggc cgtccggggc gtcggcagcc
     3301 ggtctcgccg cccccggggg gacgaggagc cattggcgcc cgtggcgctt cccgagatcc
     3361 gccgccgagg agaggtcgaa gctctcggcg agggccttgt ccggcccgag gacccaccgc
     3421 cccggttcgc ccgcctgcca gcgcgctgcg gcggccccct gcatctcggg cggcatcagg
     3481 taggggaagt ggtgcgtcgg ccgccgcgcg aagaggagga actgctcgcg gtaggcgacg
     3541 agggcgaggg cgtcgccggg cgggacccgc gcctccgccg cctcgacgac cgacttcggc
     3601 gtccgcgagc cgttcaggag cgggttcacg ccgaagccga cgacgagcca gacgaccgcg
     3661 aggacggagg tgacggccgc gagggcgaag tgccggaaac ggaggacgag cgccgtcgcg
     3721 gcgatgccga ggacgagcgc ggcgggccag gggttcgcga tctcctgctc cgcgagcttc
     3781 tgcccgaccc tcggcacgag gagggccgcg agcgccgcga ggagcccgac cgccgcgacg
     3841 aaggcggcaa ggcccgtgaa gagccgccgc ggccacgcct tccgaaggag ctccggcagg
     3901 agcggcgcgg cgaggagggc gagcgcggga gtggccggag tgatgtagac gccgcgcttg
     3961 ccgggcgagg cgctgaagaa gacgaggacg aggaggaggt agccgagaag gaccgggacc
     4021 cggcggtccc cggcgcgcac ctcgcggacc cacgcgggga cgagccacgg caggaggagc
     4081 gagagcggga gccagaacgg ggggatcacc ttgacgaggt agtcccacca cggctcgacg
     4141 tggtgccacg cggcgccgta gcgcgtgacg gtctgcttga agaggatgtt gttccgatag
     4201 gcctcgagcg cggggtcgcc gctctgcgcg accgcgacga gcatcgggac gccccagagg
     4261 gccacggccg cgaggagggc gagcggcccg gcgagcccct tcagccaggc ggagaggggc
     4321 gcgcggcgga tctcggcccg atgcgttgag agcgccggga cgagggcgaa gagggcgagg
     4381 atgccgaccc ccttcgtgat gatgccgagc cccgccgcaa accatcccag atagtaccag
     4441 cgccacccgc ccccgaggag gaggaagcgg acgaacgcgt agacgccgag cgtgatccag
     4501 agcgtgacga gggcgtcgat ctgggccgtc cgcgcctgga gcgtgaactg aaccgtcagg
     4561 aggaggagga gcccggcccg ccaggccgtc gcgcggtcgt ggaggcgccg cgcgaggtcg
     4621 aagacgaggg cgagcgtccc gaggccggcg aggagcgacg ggaggaggaa cgcgacccgg
     4681 agcgagcccg tcagcttcag gaagagggcg atcgcccaca tgaagagggg cggcttgtcg
     4741 gggtagagct ccccgccgcg gaccgggaag agccactgcc cgctctcgac catctgccgg
     4801 gccacgagcg cgaagcgcgg ctcgtcggcg ggccacggat cgcgcaggcc gaggccggct
     4861 gcgaagagga cggtcgcgag gaggaggagg aggaggagcg cccggcggtc gtcccgcggc
     4921 ccctcgagga gggcctcgtc gggcgtcacg ccccgtcccc ggggtcggtc ccggcttcca
     4981 ggtggcgcgc cgccttgatg agccagaggt tcctgacgta gaccacggcc cccgcgctct
     5041 ggccgaggat gaagacgggg tccttccggt agatcgcgta ggcgaggagg agggccgagc
     5101 cgccgaggct gaagtaccag aacgcgacgg ggatgacgct tcgcttctga cgctcggagg
     5161 cgatccactg gatgatgaag cgggcgctga agaggagctg gccgaagagg ccgagtccga
     5221 cccagatacg ctcgacggcg accgtcacgg gaggacctcc ttcacgagag gggacttcgc
     5281 ccgccgcttc agccacgcga cgccgaggac gtcgacgagg ccgacccaga ggcggttcca
     5341 gacgccgtag ttcgagcggc cggccatccg ggggcggtgg ttcacggggt ggacgagcat
     5401 gacgccgccg agcgactgga cgagcgtcgg aacgtagcgg tgcatgtggt cgaacgcggg
     5461 gaggcgaagg taccagtcga ggcggacgga cttcaggccg cagccgacgt cggggacgcc
     5521 gtccttcagc atcgcgtcgc ggaacccgtt cgccaccttc gagctgacgc gcttgatcca
     5581 gtcgtcccgg cggttcaccc ggtggccgat gacgccgacg acgcgcgggt cgcgggcgtt
     5641 cccctcgagc gccgtccgca ccatcccggg gatgtcggcc gggtcgttct ggccgtcggc
     5701 gtcgaggacg acgagccact cgccccgcgc ggcgcgggcc cccgtcagga ccgcgaagct
     5761 ctggccggtg ctcttctcgt gccggaggat tcgaagccgg tcgaacccct cggcggcgag
     5821 ggccttcagc gtctcccccg tcgcgtcggt cgagccgtcg tcgacgtaga tcagctcgta
     5881 gtcgaagacg ccgtcgaggg cctgccggac ctcggcgacg agggggcgga cgttctcggc
     5941 ctcgttccga gccgggatga cgaccgagag aaaaggttgt tcgggcatgc cgtctttcgt
     6001 cgcgcgcagg agcccccgat tccggtcggc tccccgtgcg cggaaggggt gattttcgct
     6061 caatttcccg aacgtttccc gaagccggcc tccggggctc tgcgagacgg tgacggccgg
     6121 cttccgtcga cccggggacc tctccgccac cggcacgctc ccgccacgca ccggtccccg
     6181 gtggcccgcc cgtccgacgc ggcctcccgg acttgacagc tgatcccgtg ttgtgctgca
     6241 ttgcagaact ccgatagagt gccgtccatg ttccccaccg acgaggaggc gttcatgtac
     6301 cgtagccagt ccattccgga gctcgagcgg tcctgggcca acgacctgcg ctggcgggga
     6361 atcgtccgcc cgtacagcgc cgcggacgtc gaccgcctcc gaggcacgat ccagatcgac
     6421 taccccgtcg cccggatggg cgccgagcgt ctctggaacc tcctccacac cgagacgttc
     6481 gtccgggccc tcggcgccct gaccggcaac caggccgtcc agcaggtgca ggcggggctg
     6541 aaggcgatct acatgtcggg ctggcaggtg gcgggcgacg cgaacgacgc cctcacgatg
     6601 taccccgacc agagcctcta cccggtcacg agcgtcccga ccgtcatccg gcggatcacg
     6661 aacgcgctga tgcgagccga ccagatccag cacatggagg ggcggtccga cgtctactac
     6721 ttcgcgccga tcgtggccga cgcggaggcc gggttcggcg gcccgctgaa cacgttcgag
     6781 ctgatcaagt cgatgatcga ggcgggcgcc ggcggcatcc acctcgagga ccagctctcc
     6841 tccctcaaga agtgcggcca catgggcggc aaggtcctcg tcccgatcca cgacttcatc
     6901 tccaagctca tcgccgcccg cctcgcggcc gacgtcctcg gcgtcccgac ggtcttcatc
     6961 ggccggaccg actccctcgg cgcgagcctc atccgcggcg acatcgacaa ggtcgaccag
     7021 gagttcctga ccggcgagcg gacgtcggac ggcttctaca tcgtcaaggg gagcctcgag
     7081 tacgccgtcg cccgcgcctg cgcctacgcc ccgtacgtcg acgtcgtctg gtgcgagacc
     7141 tcgacgcccg acatcggcga ggcgcgcgcg ttcgcccagg gggtccacga gaagttcccc
     7201 ggcaagctcc tcgcctacaa ctgctcgccg tcgttcaact ggaagaagaa gctctccgac
     7261 tcggagatcg ccgcgttcca ggagg
//
LOCUS       DAOTEZ010000203         7276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_7283_length_7276_cov_7.357984, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000203 DAOTEZ010000000
VERSION     DAOTEZ010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7276
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7283_length_7276_cov_7.357984"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..350
                     /locus_tag="P5164_11480"
     CDS             57..350
                     /locus_tag="P5164_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HRY44554.1"
                     /translation="MTEALGMIETRSFPAVVEAADAAVKAAKVELVSYEKTGGGYVTV
                     IVRGDVAAVKAACDAAQVAGGRVGEMVAVHVIARPHANVDAVLPLGRKPAEAK"
     gene            375..662
                     /locus_tag="P5164_11485"
     CDS             375..662
                     /locus_tag="P5164_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HRY44555.1"
                     /translation="MQLAKVVGSVVATRKEESLSGLKLLLVRPVDEEGRDGTAVLVAA
                     DAVGAGPGEVVLIAAGSSARQTVATDKRPVDAVVMAIVDSWDVGGDVKYRK"
     gene            664..2112
                     /locus_tag="P5164_11490"
     CDS             664..2112
                     /locus_tag="P5164_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013400810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HRY44556.1"
                     /translation="MAARLTESEIEAIARRIVSDLDGKPATAPPAGGAAAGDSGASMG
                     IHPTVNAAVEAARKAQPRFVALPLRVRAKVIEAIRATMRENGAALAKAAWEETGLGRS
                     EDKVVKNALVTERTPGLEDLYPSAVTGDHGLSLIEPAPFGVIGALTPSTNPTSTIICN
                     AIGMLAAGNAVVFSVHPSAKECSIRTIALLNKAIVSAGGPPDVVTGVSVPSLESAQEV
                     MKHPGIRLLVVTGGGAVVKAAMASGKRAICAGPGNPPVVVDETADLAKAGRDIVLGGS
                     TDNNVICVDEKEVLVVAKVADELIRAMVAAGAVLIGKDRLKELEKAIFTKMAGPREHA
                     WVDKDLIGKNANVILRRMGISAPDSVRQIVVEVDADHPLLWTEQMMPVMPVCRVPSVD
                     AAIDLAVPVEGRNRHTFVMHSRNLDALSRMAKECDASIFVKNGRSQAGLGLDGEGFCS
                     FTIASPTGEGLTSPRSFSRWRRCVLVDNFRIT"
     gene            2120..2731
                     /locus_tag="P5164_11495"
     CDS             2120..2731
                     /locus_tag="P5164_11495"
                     /inference="COORDINATES: protein motif:HMM:NF013129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HRY44557.1"
                     /translation="MKRAAALAVLDFAEVPAGLDATDAMLKKAPVAFFRSGTVTAGRY
                     LTLLGGTPASVEEALAEGLARAGGHLLDHLYLADVHPRLLDALEGRRGEDGTGALLIL
                     ETATASSVVLACERALKGTLVDLVEVRLSDAGLAGKGLALFRGDLHELEAAVEIVTSV
                     LPPAAGLSYRVLARPHDALAREAALGTAFAASRFVDLGGEEAS"
     gene            2731..3015
                     /locus_tag="P5164_11500"
     CDS             2731..3015
                     /locus_tag="P5164_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HRY44558.1"
                     /translation="MLLGRVIGRVVPCVVTPGLEGVPMLLLQPLGKDGAPKGKALVAA
                     DPTRMAGEGELVAYEGGREAALLLDPWFVPVDHAVVGIVDALHLPAKEGA"
     gene            3012..3281
                     /locus_tag="P5164_11505"
     CDS             3012..3281
                     /locus_tag="P5164_11505"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HRY44559.1"
                     /translation="MITGRVIGEITATIRHPFFEGKKMLVVARTGADGAETGDYVVAI
                     DAVGVGPGDPVLVLDEGNGARQVVGSATAPVRSVVVGFLDRVDLA"
     gene            3283..3729
                     /locus_tag="P5164_11510"
     CDS             3283..3729
                     /locus_tag="P5164_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44560.1"
                     /translation="MDAPPPPVPPLLPPPPPLPPAPPPVFAVPVVEPACLRWQRLYLG
                     AMAAIYLACVAGGALLLVFRQEIADWPPRDEPWEIALTGGLLLVLGALFLALYVAGFF
                     LPRRPWAWTAHLVLIAFGLTSCCTLPAAIPLLVAFLRRDTQAWFGR"
     gene            3783..4715
                     /locus_tag="P5164_11515"
     CDS             3783..4715
                     /locus_tag="P5164_11515"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRY44561.1"
                     /translation="MPRPPRLDSTGIVTHVVARGNERRPLFRDDADRTKYLDLLGEAC
                     GRYGARAFAWCLMPNHVHLALQTGSEPISRLVHDVHSRYAQYFNRRWERSGHLFQGRF
                     QGIVVDREAYLLEVVRYIHRNPVKARLASRPEEFPWSSHGAYLSTGPAWLASSEVLGL
                     LAGGRPKARRLFQEFVSGSTAGRYDPDEARFGAVVGGEDFVRAALAGAGREDLVRRSL
                     TVDAVVKAVAAHEGVSVAELAGPSRLRSLSRVRSLCALLGRELGRIPLAETARSFGRD
                     PSTISRDVAHLERRLAEDPAEARRLAELRKLLSA"
     gene            4861..5559
                     /locus_tag="P5164_11520"
     CDS             4861..5559
                     /locus_tag="P5164_11520"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRY44562.1"
                     /translation="MRGLLFFDLDDTLLDHAGAEARAQEETFEGRRGVFGSLEFCEWL
                     AAYRRANGRLWAAYGRGEIGREELHRRRFADPLEELGRDPAAADEAGAFYLERYRGAW
                     RLNEGAEELLAAAAERGEVGVLSNGFRELQRAKLARFRLERWVKHIVLSEEAGAMKPA
                     RAIFDAAVRSACGEGGADGRRKLYLGDHYETDAVGAKGAGWLPVLYNPSRRPLPGPVL
                     HVARLTDAIPLLAG"
     gene            complement(5586..>7276)
                     /locus_tag="P5164_11525"
     CDS             complement(5586..>7276)
                     /locus_tag="P5164_11525"
                     /inference="COORDINATES: protein motif:HMM:NF022840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2813 domain-containing protein"
                     /protein_id="HRY44563.1"
                     /translation="HSTRRRDRDVPLRRIEIEGFRGIRRMTLDLDETTTLIGENSCGK
                     TSVLDALELVFGHRGAQPAFSPLDFHLPVGTAAPVERIAMRFTFSADGGRETVAELTA
                     ARTPDGSPAPATARLTDREGRDLAPGDEEAVASFRRRHPVLRVRFNRPPRPAPPGLEP
                     EDASSPDETSAPAVPAIGGGGEGEVTRKLRRTVESAYRKFSGSWRPHPEELREPIAAA
                     RALAERWIERLRLAPRAPGGRTTEILAETPVLLGGTNPLRTESREGTGMQSLALLMLL
                     GAVLDAENGSPVEAGAEPLVLIEEAEAHLHPILAARVFGLIDRVAGQKILTTNSGDLL
                     ASVPLHAIRRLVRNGGGVAVHGLAEGALTVDEMRRVGYHIRVNRASSVFARCWLLVEG
                     ETESWLLPEIARLIGYDLPAEGVRCVEFAQCGIAPLVKMARGLGIEWHLLADGDDAGR
                     HYVRAARSFLQGEPDRERVTGFRERDVEHHLWGAGYAGVYRAAAQRAGMLPSLFGRRR
                     IPTTAIIDRALKAHGKPRMALEVAEAMAAPDSPGVPRLLASVVEKTVRLARFST"
BASE COUNT          940 a         2674 c         2671 g          991 t
ORIGIN      
        1 aacgcggtaa cgcgacgggt cggcgccgtg cgacgatccg aggagaggaa agagccatga
       61 ccgaagccct cgggatgatc gagacgaggt ccttccccgc ggtcgtcgag gccgccgacg
      121 ccgccgtgaa ggccgccaag gtggagctgg tctcctacga gaagacgggc ggcggctacg
      181 tcaccgtcat cgtccgcggc gacgtcgccg ccgtgaaggc cgcgtgcgac gcggcgcagg
      241 tcgccggcgg ccgcgtcggc gagatggtcg ccgtccacgt catcgcccgc ccgcacgcga
      301 acgtcgacgc cgtcctcccg ctgggccgca agcccgccga ggcgaagtag cgccccgggg
      361 cgcgggagag cgccatgcag ctcgcgaagg tcgtcgggag cgtcgtcgcg acgcgcaagg
      421 aggagagcct ctcggggctc aagctgctcc tcgtccgccc ggtcgacgag gaggggcgcg
      481 acgggacggc cgtcctcgtc gccgccgacg ccgtgggcgc cggcccgggg gaggtcgtcc
      541 tgatcgccgc cggcagctcg gcgcggcaga ccgtcgccac cgacaagcgc cccgtcgacg
      601 ccgtcgtcat ggcgatcgtc gactcctggg acgtcggcgg cgacgtgaag tacaggaagt
      661 gacgtggccg cgcgcctgac cgaatccgag atcgaggcga tcgcccgccg catcgtctcg
      721 gacctcgacg gcaagcccgc gaccgcgccg ccggccggcg gcgcggccgc aggggactcc
      781 ggagcctcga tggggatcca cccgacggtg aacgccgccg tcgaggcggc gcggaaggcg
      841 cagccccgtt tcgtcgccct cccgctgcgc gtccgcgcga aggtgatcga ggcgatccgc
      901 gcgacgatgc gcgagaacgg cgccgcgctc gcgaaggcgg cgtgggagga gaccgggctc
      961 gggcgctccg aggacaaggt cgtcaagaac gccctcgtca cggagaggac tcccggcctc
     1021 gaggacctct acccgtcggc ggtgacgggc gaccacggcc tctcgctcat cgagccggcg
     1081 ccgttcggcg tcatcggcgc gctcacgccg tccacgaacc cgacgtcgac gatcatctgc
     1141 aacgcgatcg ggatgctcgc cgccgggaac gccgtcgtct tctcggtcca cccgtccgcg
     1201 aaggagtgct ccatccggac gatcgccctc ctcaacaagg cgatcgtctc cgcgggcggc
     1261 ccgcccgacg tcgtgacggg cgtctccgtg ccgtcgctcg agtcggcgca ggaggtgatg
     1321 aagcacccgg gcatccgcct cctcgtcgtc acgggcggcg gggcggtcgt gaaggccgcg
     1381 atggcgagcg gcaagcgcgc catctgcgcc gggccgggga acccgcccgt cgtcgtcgac
     1441 gagacggccg acctcgcgaa ggcggggcgc gacatcgtcc tcggcggctc gaccgacaac
     1501 aacgtcatct gcgtcgacga gaaggaagtc ctcgtcgtgg cgaaggtggc cgacgagctg
     1561 atccgcgcga tggtcgccgc cggagccgtc ctgatcggga aggaccgcct gaaggagctc
     1621 gagaaggcga tcttcacgaa gatggccggg ccgcgcgagc acgcctgggt cgacaaggac
     1681 ctcatcggga agaacgcgaa cgtcatcctc cggcgaatgg ggatctccgc ccccgactcc
     1741 gtgaggcaga tcgtcgtgga ggtcgacgcc gaccacccgc tcctctggac cgagcagatg
     1801 atgccggtca tgcccgtctg ccgcgtcccg agcgtcgacg cggcgatcga cctcgcggtc
     1861 cccgtcgagg ggcggaaccg gcacaccttc gtcatgcact cgcggaacct cgacgccctc
     1921 tcgcggatgg cgaaggagtg cgacgcctcg atcttcgtca agaacggccg gtcgcaggcg
     1981 ggcctcggcc tcgacggcga ggggttctgc tcgttcacga tcgcgagccc gacgggcgag
     2041 gggctcacgt cgccgcgcag cttctcgcgc tggcgccgct gcgtcctcgt cgacaacttc
     2101 cggatcacct gagcgtccca tgaagcgcgc cgccgccctc gccgtcctcg acttcgcgga
     2161 agttcccgcg gggctcgacg cgacggacgc gatgctgaag aaggcccccg tcgccttctt
     2221 ccgctcgggg acggtgaccg ccggccgcta cctgacgctc ctcggcggca cccccgcctc
     2281 cgtcgaggag gcgctcgccg aggggctcgc ccgcgcggga ggccacctcc tcgaccacct
     2341 ctacctcgcc gacgtccacc cgcgcctcct cgacgccctc gaggggcgcc ggggcgagga
     2401 cgggacgggc gcgctcctga tcctcgagac ggcgacggcc tcgtccgtcg tcctcgcctg
     2461 cgagcgggcg ctcaagggga cgctcgtcga cctcgtcgag gtgcgcctct cggacgccgg
     2521 cctcgccggc aaggggctcg ccctcttccg cggcgacctc cacgagctcg aggcggcggt
     2581 cgagatcgtg acctcggtcc tcccgcccgc ggcgggtctc tcgtaccggg tcctcgcccg
     2641 cccccacgac gccctcgcgc gcgaggccgc gctcggcacc gcgttcgcgg cgagccgctt
     2701 cgtcgacctc ggcggcgagg aggcctcgta gtgctcctcg gacgcgtgat cggccgcgtc
     2761 gtgccgtgcg tcgtgacgcc gggcctcgag ggggtcccga tgctcctcct ccagcccctc
     2821 ggcaaggacg gcgccccgaa agggaaggcc ctcgtcgccg ccgacccgac gcggatggcg
     2881 ggggagggcg agctggtcgc ctacgagggg gggcgcgagg cggcgctcct cctcgacccg
     2941 tggttcgtcc cggtcgacca cgccgtcgtc ggcatcgtcg acgcgctcca cctccccgcg
     3001 aaggagggag cgtgatcacc gggcgcgtga tcggcgagat cacggcgacg atccgccacc
     3061 ccttcttcga ggggaagaag atgctcgtcg tggcgcgcac gggggccgac ggcgccgaga
     3121 cgggcgacta cgtcgtcgcc atcgacgcgg tcggcgtcgg gcccggcgac ccggtcctcg
     3181 tcctcgacga ggggaacggc gcgcgccagg tcgtcggatc ggccacggcg cccgtccgct
     3241 cggtcgtcgt cgggttcctc gaccgcgtcg acctcgcctg acgtggacgc gcctccgccc
     3301 cccgtcccgc ctctcctccc gccgcccccg cctctcccgc cggcgccgcc ccccgtcttc
     3361 gccgtccccg tcgtcgagcc tgcctgcctc cgctggcagc gcctctacct cggcgcaatg
     3421 gcggcgatct atctcgcgtg cgtcgctggc ggggcgctcc tcctcgtctt ccgtcaggag
     3481 atcgccgact ggcctccgcg cgacgagccg tgggagatcg ccctgacggg cggactcctc
     3541 ctggtcctcg gtgccctctt cctcgccctc tacgtcgccg gcttcttcct cccacgccgg
     3601 ccctgggcct ggaccgccca cctcgtcctc atcgccttcg gcctcacgag ctgctgcacg
     3661 ctccccgccg ccatcccgct cctcgtcgcc ttcctccgcc gcgacaccca ggcctggttc
     3721 ggccgctgac gtcgccctcc ccgagaccgg gtgccaggcg tgaattcgtg tattgttgtg
     3781 gcatgccgag acctcctcgc ctcgattcga caggcatcgt cacgcacgtc gtcgcccgag
     3841 ggaacgagcg ccggcccctc ttccgcgacg acgccgaccg cacgaagtac ctcgacctcc
     3901 tcggcgaggc gtgcggtcgg tacggggcgc gcgcgttcgc gtggtgcctc atgccgaacc
     3961 acgtccacct cgcgcttcag acggggagcg agccgatctc ccgcctcgtc cacgacgtcc
     4021 actcgaggta cgcgcagtac ttcaaccggc gctgggagcg ctccgggcac ctcttccagg
     4081 gacgcttcca ggggatcgtc gtcgaccgcg aggcgtatct cctcgaagtc gtccgataca
     4141 tccaccggaa cccggtcaag gcgcgcctcg cgtcccggcc ggaggagttc ccgtggtcga
     4201 gccacggcgc ctacctctcg acggggccgg cgtggctcgc ctcgtcggaa gtcctcgggc
     4261 tcctcgccgg cggacgtccg aaggcacggc ggctcttcca ggagttcgtc tccggctcga
     4321 cggccgggcg ctacgacccc gacgaggcac ggtttggggc ggtcgtgggg ggggaggatt
     4381 tcgtccgcgc ggcgctcgcc ggggcggggc gcgaggacct cgtccgtcgc tcgctcaccg
     4441 tcgacgccgt cgtgaaagcc gttgccgcgc acgagggagt ctccgtggcc gagctggcgg
     4501 ggccgtcgcg cctccggagc ctctcccgcg tccggagcct ctgcgcgctc ctcgggcggg
     4561 agctcggaag gatcccgctg gccgagacgg cccggtcctt cgggcgggac ccctcgacga
     4621 tctcgcggga cgtggcccac ctcgagcgcc gcctcgccga agaccccgcg gaagcgcgcc
     4681 ggctggcgga gctgcgaaag ctgctctcgg cctgaagccg atcggggccg ggccggacgg
     4741 cgccgatcgc gagccgtggc tcagagggcg tacgaaggcg gacgcccgag cgcgggctcg
     4801 aaaacaatac acgatttcac gcctggcacc accggtgggg gagtgtggga ggatgacggg
     4861 gtgagggggc tgctcttctt cgacctggac gacacgctgc tcgaccacgc gggggcggag
     4921 gcgcgggcgc aggaggagac gttcgaggga aggaggggcg tcttcggctc cctggagttc
     4981 tgcgaatggc ttgctgccta ccgccgcgcg aacgggcgcc tctgggcggc ctacggccgc
     5041 ggggagatcg ggcgggagga gctccaccgg cgacgcttcg ccgatcccct ggaggagctc
     5101 ggacgcgacc cggcggcggc cgacgaggcg ggcgccttct acctcgagcg ctaccgcggg
     5161 gcgtggcggc tgaacgaggg ggccgaggag ctcctcgccg ccgccgcgga gcggggcgag
     5221 gtcggggtcc tctcgaacgg cttccgggag ctgcagcgcg cgaagctggc gcgcttccgg
     5281 ctggagcggt gggtgaagca catcgtcctc tccgaggagg cgggggcgat gaagccggct
     5341 cgcgcgatct tcgacgccgc ggtccggtcg gcgtgcggcg agggcggagc cgacggtcgc
     5401 cggaagctct acctcggaga ccactacgag accgatgccg tcggcgcgaa gggggcgggt
     5461 tggctcccgg tcctctacaa cccctctcgc cgtcctcttc cggggccggt gctgcacgtg
     5521 gcgcgcctca ccgacgccat cccgctcctc gcgggatagg cccccggggc cggtctcccg
     5581 cccgttcagg tggagaagcg ggcgaggcgg accgtcttct cgacgaccga cgcgaggagg
     5641 cgcggcaccc ccggggaatc gggcgcggcc atcgcctcgg cgacctcgag cgccatgcgc
     5701 ggcttcccgt gcgccttcag cgcgcggtcg atgatcgccg tcgtgggaat gcggcgccgg
     5761 ccgaagaggg acgggagcat cccggcgcgc tgggcggccg cccggtagac gcccgcgtac
     5821 ccggctcccc agaggtggtg ctcgacgtcc cgctcgcgga agccggtgac acgctcgcgg
     5881 tcgggctctc cctggaggaa ggagcgggcc gcgcggacgt agtgccggcc cgcgtcgtcg
     5941 ccgtcagcga ggaggtgcca ctcgatcccg aggccgcgcg ccatcttcac gagcggcgcg
     6001 atcccgcact gggcgaactc gacgcaccgg accccctcgg cggggaggtc gtacccgatg
     6061 agccgggcga tctcgggcag gagccacgac tcggtctccc cctcgacgag gagccagcag
     6121 cgggcgaaga ccgaggaggc ccggttgacg cggatgtggt agccgacgcg ccgcatctcg
     6181 tcgaccgtca gcgccccttc cgcgaggccg tggacggcca ctccgccccc gttccggacg
     6241 aggcggcgga tcgcgtggag ggggaccgag gcgaggaggt cgcccgagtt cgtcgtcagg
     6301 atcttctgcc cggcgacgcg atcgatcagg ccgaagacgc gtgcggccag gatcgggtgg
     6361 aggtgggcct cggcctcctc gatcaggacg agcggctcgg cgcccgcctc gacgggggag
     6421 ccgttctcgg cgtcgaggac ggcgccgagg agcatcagga gcgcgaggct ctgcattccg
     6481 gtcccttcgc gcgactcggt ccgaaggggg ttggtgccgc cgaggaggac cggcgtctcg
     6541 gcgaggatct cggtcgttcg gccgccgggc gcacggggcg ccaggcgaag ccgctcgatc
     6601 cagcgctccg cgagcgcgcg ggctgcggcg atcggctccc ggagctcctc gggatggggc
     6661 ctccaggagc ccgagaactt ccggtaggcg ctctcgaccg tccgccggag cttccgggtc
     6721 acctcgccct ccccgccgcc cccgatcgcc gggacggccg gggcggacgt ctcgtccggg
     6781 ctcgatgcgt cctccggctc gagccccggg ggcgcggggc ggggcggccg gttgaagcgg
     6841 acgcgcagga cgggatgccg gcgacggaac gacgcgacgg cctcttcgtc ccccggcgcg
     6901 aggtcgcggc cctcgcggtc ggtgaggcga gccgtggcgg gagcggggga gccgtcgggg
     6961 gtccgggcgg cggtgagctc ggcgacggtc tcgcgcccgc cgtcggcgga gaacgtgaac
     7021 ctcatggcga tccgctcgac cggagccgcc gttccgacgg gcaggtggaa gtcgaggggc
     7081 gagaaggcgg gctgcgctcc gcggtgcccg aagacgagct cgagcgcgtc gaggacgctc
     7141 gtcttgccgc aggagttctc cccgatcagc gtggtcgtct cgtccaggtc cagcgtcatc
     7201 cgccggatcc cgcggaagcc ttcgatctcg atgcgtctga gcggcacgtc ccgatcgcgt
     7261 ctccgcgtgg agtgta
//
LOCUS       DAOTEZ010000204         7250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_7329_length_7250_cov_5.658374, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000204 DAOTEZ010000000
VERSION     DAOTEZ010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7250
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7329_length_7250_cov_5.658374"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1786)
                     /locus_tag="P5164_11530"
     CDS             complement(<1..1786)
                     /locus_tag="P5164_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44564.1"
                     /translation="MLLPRLEELFAFALGASLVRQFLWSVPGTVVPLLASALGGVAAT
                     VVVRRSAASRPTPPRLFWLVVALPILALWALRAPLPDLGYDTLNYHLMHGQKAWMGPL
                     YPPGDFYPYYFPFLNPTGDMVTALFRFVLGYRGGTVASALALVWTGEVLWRLLSPVLK
                     EERVRAAAVLAAIATEGTLWEVSGYMVDLLPLPILLEALTVALSPSAEAVRLPRRSAV
                     LGLLLGTAVALKLTNLVFALPVGLVALLRWRSGFSSVRPAIAALGAGALAFVLPVAPH
                     ALVLTLSHGNPVFPHFNARFQSPDFPPLDIKDLRWGPVSARDALAWPVTSAFHPERLS
                     EFLLTTGRLAAGWIVALGALLLLRRDGFVRAVAFVAVAGGVLWSFGTGYYRYGLFGEL
                     AGSLVVVLVAARLAGSPGPRSERRARQPWTLPRAVAAGLAFVLAAQTGRAFFLALRSD
                     WSGRPTVFHALRDSSREVRHLFVDRDLSRFVPPEWRATLAAEPVWVDVTPKVNGIMTF
                     LSPRSPMIGLQMEDLLAVQPNLVRLDEALRACAGRPAFSLAFAEDAARAEERLFRLGF
                     PVLGKTPVSFPFFSEERRIDLVLFSVALP"
     gene            complement(1819..2211)
                     /locus_tag="P5164_11535"
     CDS             complement(1819..2211)
                     /locus_tag="P5164_11535"
                     /inference="COORDINATES: protein motif:HMM:NF021575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HRY44565.1"
                     /translation="MLTNTLRVQVAGVLLALAAFAVVIELVRRRRLTEWQSIVWLTAA
                     VAIGTFAVYRRLQFALAEVVGIYYPPVLILAAFVFGGLLILLLQSVALTRLEERNRRL
                     AQDVALLRERLDRVESSRAEQPAPEDEP"
     gene            complement(2240..2944)
                     /locus_tag="P5164_11540"
     CDS             complement(2240..2944)
                     /locus_tag="P5164_11540"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY44566.1"
                     /translation="MSRPVIALVLAYNEEEALPGVLSELARECPGWSVVVVSDGSTDG
                     TGACARRAGAALLQLPFNAGVAAAEQTGFLYAGSLGARVVVRLDGDGQHPAAEARRVV
                     AALEETGADVAIGSRFLRPGGFRSTFARRAGIRWLSFLLRLFGGLRIADPTSGLRAFG
                     GRAIALLSRVYPHGYPEPESNLLLGRAGLRVEEVPVTMRARDGGRSSLRGWSTVYYPV
                     KVSFALLVEALRERVR"
     gene            3177..4265
                     /locus_tag="P5164_11545"
     CDS             3177..4265
                     /locus_tag="P5164_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44567.1"
                     /translation="MQVRYGELPLDALRALFDLEGLRALFLDLVTRLDGDVEEALGVM
                     RRLQEQGLLPADLDLDALLEELEKNGLVSRDADGRLSLTLPGERQVRRAALEEVFSSL
                     SRSGAGLHALPREGAGTEPLEETRPWEFGDDLSRLDGIRTVTNAARRDPEKLAILPED
                     LEVHETEHQAACATAIAIDVSHSMVLYGEDRFTPAKKVALALTELILSKYPKDTIDVV
                     LFGDDAERVPIESLSKARVGPFHTNTRAGLALCRGLLLARRSRNRQIFLVTDGKPSCI
                     RENGKLYKNPFGLDLKIVNRTLDEAERCRREGIQLTTFMLATDPGLVEFVDTLTRVAR
                     GRAYYASSGDLATFVFRDYLRNRRRLFR"
     gene            complement(4279..5130)
                     /locus_tag="P5164_11550"
     CDS             complement(4279..5130)
                     /locus_tag="P5164_11550"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY44568.1"
                     /translation="MTERERWTPLSMTLAGIPLRGVSVAGRETWFRVPSLDLAFDVGR
                     SPAEVVPVPSLFLSHAHLDHAGGLAYWASQRRLLRLAPARVFVDPVTVPAWMQILTLH
                     AGLEGVEYHVAVEGLAPGDAVDLRRDLSVTAFRADHRVPTLGFVASEVRRRLVPAWQG
                     RSEDDIREALARGEPVTQRSPRPLVAFSGDTGIGFFSSAPKEALEAKILFLECSFVAP
                     EDASRARHYGHLHVDDIAERAELFENEAVVLTHLTLRATPDELRRAIARRLPPALASR
                     AVPFLPG"
     gene            5220..6818
                     /locus_tag="P5164_11555"
     CDS             5220..6818
                     /locus_tag="P5164_11555"
                     /inference="COORDINATES: protein motif:HMM:NF012255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HRY44569.1"
                     /translation="MVLFNHSTRELTAKIVYYGPGLCGKTTNLRLLHEKLEQGTTGRL
                     LSLSTAQDRTIYFDLLPVELGNVKGYTVRFQLCTVPGQVFYNETRKLVLRGVDGIVFV
                     VDSQWSMLSHNLESFQNLRENMAELDQTFDETAVVIQYNKRDLPGVLSVQALQESLGF
                     QDYPFVEAVAASGKGVVETFKLVSKMTFVQLLRRLQRSPDGEKLGPEWVAPPSEDASR
                     TTRIPAVGAELLALTSTVGPAPAPPTESPFETTASWPSMPRGEVFGDATVPPQPGLPP
                     APAPTDVPFEPAPLPDSADDTLTDGPPVTAVAEVARAESPAPPEPPEPESSSLPEPTE
                     GPGPEVAAPEAPAAPEPLAVPPPAPEAEPPSLLVAAPPPPPPQPEPPPAAAVWAEAFA
                     ALSSRIDGLASARSEETARLETLIREGRELLEERLDGIQAPIERSLDGLAASLEERFS
                     ALERAFGSRIEELRTVLEARLGVLEDAVARLESSDRLEEVRRETADVKRLVESKLDEA
                     GGQSRRLGAFLRRALDELEPPAGD"
     gene            complement(6826..>7250)
                     /locus_tag="P5164_11560"
     CDS             complement(6826..>7250)
                     /locus_tag="P5164_11560"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRY44570.1"
                     /translation="GLTPRGERRIVPGAEESVKPERRRSPRVRAPAGAEGLIRSTLQV
                     QVEDVSKDGVRFRLSGPIRPGSTYAFHASLEGLDLAAPIRITRCKAIPPAKGAGRGIV
                     YEAGAEFVWEKPADEERVVSWLAVRGAAAGRIRAELEG"
BASE COUNT         1118 a         2608 c         2647 g          877 t
ORIGIN      
        1 gagggagggc gaccgagaag aggacgaggt cgatgcggcg ctcctccgag aagaacggga
       61 acgagacggg ggtctttccg aggacgggga agcccagccg gaagaggcgc tcctcggccc
      121 gggccgcgtc ctccgcgaac gccagggaga acgccggacg ccccgcgcag gcccggagcg
      181 cttcgtcgag ccggacgaga ttcggctgca cggccaggag gtcctccatc tgcaggccga
      241 tcatcggaga cctcggcgag aggaacgtca tgatcccgtt gaccttcggc gtgacgtcga
      301 cccagacggg ctcggcggcc agcgtcgccc tccattccgg cgggacgaat cgggagaggt
      361 cgcggtccac gaagaggtgg cgcacctcgc gcgacgagtc ccggagggcg tggaagaccg
      421 tcggacgccc gctccagtcc gaccggagcg cgaggaagaa cgccctcccg gtctgggcgg
      481 cgaggacgaa ggccagaccg gcggccaccg cccggggcag cgtccacggc tgcctcgcac
      541 gcctctccga acgcggcccg ggagatcccg cgagccgggc cgcgacgagg acgacgacga
      601 ggctccccgc gagctcgccg aagaggccat atcggtagta gcccgtcccg aagctccaga
      661 ggacgccccc ggcgacggcc acgaaggcga ccgcccggac gaacccgtcc cggcggagca
      721 ggaggagggc gcccagagcg acgatccacc ccgccgcgag ccgccccgtc gtcagcagga
      781 actcgctgag ccgctccggg tggaacgcgg aggtgacggg ccaggcgagt gcgtcccggg
      841 ccgagacggg cccccagcgc aggtccttga tgtcgagcgg cgggaagtcg ggcgactgga
      901 agcgcgcgtt gaagtgcggg aagacggggt tcccgtggct cagggtcagg acgagcgcgt
      961 gcggcgcgac gggcaggacg aacgcgaggg cccccgcccc gagggcggcg atcgccggac
     1021 ggacggagct gaagccgctc cgccagcgca ggagcgcgac gaggccgaca gggagggcga
     1081 agacgaggtt cgtgagcttc agcgcgaccg ccgtaccgag gaggagcccg aggacggcgc
     1141 tccggcgcgg cagccttacg gcttccgccg aaggcgacag cgccaccgtc agcgcctcca
     1201 ggaggatcgg gagcggcagg aggtcgacca tgtagccgga gacctcccag agggtcccct
     1261 ccgtcgcgat cgccgccagc accgcagccg ctcgcacccg ctcttccttc agcacgggag
     1321 agaggagcct ccagaggacc tcgcccgtcc agacgagggc cagcgcgctt gcgacggtcc
     1381 ctccccggta gccgaggacg aagcggaaga gggcggtgac catgtccccg gtcgggttga
     1441 ggaacgggaa gtagtagggg tagaagtcgc ccggcgggta gagcggcccc atccaggcct
     1501 tctgcccgtg catcaggtgg tagttcagcg tgtcgtagcc gaggtccggg agcggcgcgc
     1561 ggagggccca gagggccagg atcggcaggg cgacgacgag ccagaagagg cggggcgggg
     1621 tgggacggct cgccgcgctc ctccggacga cgacggtggc cgccacgccc ccgagcgccg
     1681 aggccaggag cgggacgacc gtgccgggaa cggaccagag gaactggcgg acgagcgagg
     1741 cgccgagcgc gaacgcgaag agctcctcga gccgcggcag gagcacgagg gccttccgga
     1801 ttcgcgaggc gtggggcgtc agggttcgtc ctccggcgcg ggctgctcgg ccctcgacga
     1861 ctcgacgcgg tcgagccgtt cgcgcagcag cgcgacgtcc tgggcgagcc gtcggttccg
     1921 ttcctcgagc cgcgtcaggg cgacgctctg gaggaggagg atcaggagcc ctccgaagac
     1981 gaaggccgcg aggatgagga ccggcgggta gtaaatgccg acgacctcgg cgagggcgaa
     2041 ctggaggcgg cggtaaacgg cgaacgtccc gatggcgacg gcggccgtaa gccagacgat
     2101 cgactgccac tccgtcagcc gtcggcgcct cacgagctcg atcacgaccg caaaggccgc
     2161 gagcgccagg agcacgccgg cgacctggac gcggagcgtg ttggtcagca tgcgcgcacc
     2221 gccttcgcga cgcccctcgt catcgcacac gctcccggag cgcctcgacg aggagcgcga
     2281 aggagacctt caccgggtag tagaccgtgc tccagccccg gagggacgag cggccgccgt
     2341 cgcgcgcgcg catcgtcacc ggcacctcct cgacccggag ccccgcgcgc cccagcagga
     2401 ggttcgactc gggctccggg tagccgtggg ggtagacgcg cgacaggagc gcgatcgccc
     2461 gccccccgaa cgcccgcaag ccggaggtcg ggtcggcgat ccggagcccg ccgaagaggc
     2521 gcaggaggaa cgaaagccag cggatcccgg cgcgccgcgc gaacgtggag cgaaagccgc
     2581 ccgggcggag gaaccgggag ccgatggcga cgtcggctcc cgtttcctcc agagcggcca
     2641 cgacccgccg cgcctcggcc gcggggtgct ggccgtcgcc gtcgagacgg acgacgacgc
     2701 gggccccgag gctgccggcg tagaggaaac cagtctgctc ggctgcggcc acgccggcgt
     2761 tgaagggaag ctgcaggagc gccgccccgg cccgcctcgc gcaggctccc gtcccgtccg
     2821 tggaaccgtc gctcacgacg acgacggacc agccggggca ttcccgcgcc agctccgaga
     2881 ggactccggg aagcgcttcc tcctcgttgt aggcgaggac cagggcgatg acgggccggc
     2941 tcaacccggg gccgcgcggc gacggcgcgc aagccactcc ctcagccggg ttccgtccgg
     3001 ccgtcggctc ggaatcatgc cccattctag cgtcgccgtc gggtctcacc cttctcctcg
     3061 ggatcggggt cgtggccggc gatcgggccg ggccggctcc ggggagaagt ggagtcggcg
     3121 cgccggttcc cacggcctcc cacgtcccga accgcgattc gtatgatggc ggcaagatgc
     3181 aggtccgcta cggcgagctg ccgctcgatg cgcttcgggc gctcttcgac ctcgaggggc
     3241 tccgcgcgct cttcctggac ctcgtgacgc gcctcgacgg cgacgtcgag gaggcgctcg
     3301 gggtgatgcg ccggctgcag gagcagggcc tcctgccggc ggacctcgac ctcgacgcgc
     3361 tgctcgaaga gctcgagaag aacggcctcg tctcgcgcga tgccgatggg cgcctctcgc
     3421 tcacgctccc gggggaacgg caggtccgcc gggcggccct cgaggaggtc ttctcgtcgc
     3481 tctcgcggtc cggggcgggt ctccacgcgc tgccgcgcga aggggccggg acggagcccc
     3541 tggaggagac gcgcccctgg gagttcggcg acgacctgtc gcgcctcgac gggatccgga
     3601 cggtgacgaa cgccgcgcga cgtgaccccg agaagctcgc gatcctcccg gaagacctcg
     3661 aggtccacga gacggagcac caggcggcct gcgcgacggc gatcgcgatc gacgtgtccc
     3721 actcgatggt cctctacggc gaagaccgct tcacgcccgc caagaaggtc gctctcgcgc
     3781 tcaccgagct gatcctctcg aagtacccga aggacacgat cgacgtcgtc ctcttcggcg
     3841 acgacgcgga acgggtgccg atcgagtcgc tgtcgaaggc gcgggtcggg ccgttccaca
     3901 cgaacacgcg ggccggcctc gcgctctgcc gcggcctcct cctggcccgc cgctcacgca
     3961 accgccagat cttcctcgtc accgacggca agccctcgtg catccgggag aacgggaagc
     4021 tctacaagaa cccgttcggc ctcgacctga agatcgtgaa ccggaccctc gacgaggccg
     4081 agcgctgccg ccgcgagggg atccagctga cgacgttcat gctcgcgacc gatcccgggc
     4141 tcgtcgagtt cgtcgacacg ctcacgcgcg tcgcgcgcgg ccgggcctac tacgcctcct
     4201 cgggcgacct cgccacgttc gtcttccgtg actacctgcg caaccggcgg aggctcttcc
     4261 ggtagcgtcg gcccgccgtc atccgggcag gaacggcacg gcgcgcgaag cgagggccgg
     4321 agggagccgc cgggcgatcg cccggcgaag ctcgtccggc gtcgcgcgga gcgtcaggtg
     4381 cgtcagcacg accgcctcgt tctcgaagag ctcggcccgc tccgcgatgt cgtcgacgtg
     4441 gaggtgaccg tagtgtcggg cgcgcgaggc gtcctcgggc gcgacgaacg agcactcgag
     4501 gaagaggatt ttcgcctcga gcgcctcctt gggcgcgctc gagaagaagc cgatcccggt
     4561 gtcgccgctg aaggcgacga ggggccgcgg gctccgctgc gtcaccggct cgccgcgggc
     4621 gagcgcctcg cggatgtcgt cctcggacct cccctgccac gccgggacga gccggcggcg
     4681 gacctcggag gcgacgaagc cgagcgtggg gacccggtgg tcggcccgga aggcggtgac
     4741 ggagaggtcc cgtcggaggt cgacggcgtc gcccggggcg agcccttcga cggcgacgtg
     4801 gtactcgact ccctcgaggc cggcgtggag cgtcaggatc tgcatccacg cagggaccgt
     4861 gaccggatcc acgaacacgc gcgccggcgc gagccggagg agcctcctct gcgaggccca
     4921 gtaggcgagc cccccggcgt gatccaggtg ggcgtgcgag aggaagaggc tcgggacggg
     4981 gacgacctcg gccgggcttc gaccgacgtc gaaggcgagg tcgagggaag ggacccggaa
     5041 ccaggtctcg cgcccggcga cggagacgcc ccgaagcggg attcccgcga gggtcatcga
     5101 caggggcgtc caccgctcgc gctccgtcac ggggtcagga taacgcccgg cgcgcggccg
     5161 gcgttgcagc ctatactgcc ccaccgaacc cgtcccgagc ccaacgaccg ggggagccga
     5221 tggtcctctt caaccattcc acccgagagc tgaccgcgaa gatcgtctac tacggccccg
     5281 gcctgtgcgg gaagacgacg aacctccgcc tcctccacga gaagctcgag caggggacga
     5341 ccggccggct cctctccctc tccacggccc aggaccggac catctacttc gacctcctcc
     5401 cggtcgagct cggcaacgtc aagggctaca cggtccgctt ccagctctgc acggtgcccg
     5461 ggcaggtctt ctacaacgag acgcgcaagc tcgtcctgcg gggcgtcgac ggcatcgtgt
     5521 tcgtcgtcga ctcgcagtgg tcgatgctct cgcacaacct cgagtcgttc cagaacctcc
     5581 gcgagaacat ggcggagctc gaccagacgt tcgacgagac cgccgtcgtc atccagtaca
     5641 acaagcggga cctgccggga gtcctttcgg tccaggccct gcaggagtcc ctcggcttcc
     5701 aggactatcc gttcgtggag gcggtcgccg cgagcgggaa gggggtcgtc gagaccttca
     5761 agctcgtctc caagatgacg ttcgtccagc tcctccggcg gctccagcgc tctcccgacg
     5821 gtgagaagct cgggccggag tgggtcgctc cgccgtcgga ggacgcgtcg cgcacgacgc
     5881 ggatcccggc cgtcggcgcc gagctcctcg cgctgacgag cacggtggga ccggctcctg
     5941 cccccccgac cgagtctccg ttcgagacga cggcctcgtg gccgagcatg ccgcgcggcg
     6001 aggtcttcgg cgacgccacc gttccgccgc agcccggcct gccgccggcg cccgccccga
     6061 cggacgtgcc cttcgagccg gctcctctgc ccgactccgc ggacgacact ctgacggacg
     6121 gcccgcccgt cacggcggtc gcagaggtcg cgcgggccga gtcgcccgcg ccgcccgagc
     6181 cgcccgagcc cgagagctcg tccctgcccg agccgacgga gggccccggt ccggaggtcg
     6241 ccgccccgga ggcgccagcc gccccggagc ccctcgccgt tccgcctccc gccccggagg
     6301 cggagcctcc gtcgctcctc gtcgccgcgc cgcctccacc gccgccacag cccgagccgc
     6361 cgcccgccgc ggccgtctgg gcggaggcgt tcgcggccct ctcgagccgc atcgacggcc
     6421 tcgcgagcgc ccgctccgag gagaccgccc gtctcgaaac gctgatccgc gagggacgcg
     6481 agctcctgga ggagcgcctg gacgggatac aggcgccgat cgagaggagc ctcgacgggc
     6541 tcgccgcgtc gctcgaggag cgcttctcgg cgctggagcg ggcgttcggg agccggatcg
     6601 aggagcttcg gacggtcctc gaggcgcgcc tcggcgtcct cgaggacgcc gtcgcgcgtc
     6661 tggagtcctc ggaccgcctc gaggaagtga ggcgcgagac cgccgacgtg aagcgcctcg
     6721 tcgagtcgaa gctcgacgag gcgggcggtc agagccggag gctcggcgcg ttcctgcgga
     6781 gggcgctcga cgagctcgag ccgcccgcgg gagactgagc ggggctcagc cctcgagctc
     6841 ggcccggatc cggcccgcag ccgctccccg taccgcgagc cacgaaacga cgcgctcctc
     6901 gtcggccggc ttctcccaca cgaactcggc ccccgcctcg tagacgattc cccggcctgc
     6961 gcctttcgct ggcgggatcg ccttgcagcg cgtgatccgg atcggcgcgg cgaggtcgag
     7021 gccttcgagg ctcgcgtgga aggcgtaggt cgatccaggc cggatgggcc cggagagccg
     7081 gaagcgaacc ccgtccttcg agacgtcctc cacctggacc tggagggtcg aacggatgag
     7141 gccctcggct cctgccgggg cacggacccg aggcgatcgt cttctttccg gcttcacgga
     7201 ttcctccgca ccgggaacga tccggcgttc gccgcgcggc gtcaagccag
//
LOCUS       DAOTEZ010000205         7248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_7332_length_7248_cov_6.210760, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000205 DAOTEZ010000000
VERSION     DAOTEZ010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7332_length_7248_cov_6.210760"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="P5164_11565"
     CDS             complement(<1..416)
                     /locus_tag="P5164_11565"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HRY44571.1"
                     /translation="MRWPVRALLYLLVLHAALALLALPLLRDRPGWLLAAEAAFVLSA
                     LAGALLVRRLAAPMEALRDGAELLRAGELGTRLRATGTPELDSLVALFNEMLARLQAE
                     RVRQEERQAFLEKVLEASPLGVVTLDLDGKVDVVSRA"
     gene            complement(413..1789)
                     /locus_tag="P5164_11570"
     CDS             complement(413..1789)
                     /locus_tag="P5164_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRY44572.1"
                     /translation="MKGDAAVLVVDDDSAVAASLVLLLRQAGYRPASAATPEEALARL
                     AAAPADLVLTDMNFARGTTGEEGLAFLAALRERHPGLPVVLMTAWGSIELAVRGMRGG
                     AADFVTKPWSAERLLSTVATALALSGVGPARDADVPSRDELDASAPAFAPLAGRDPKL
                     VRLLALAARVAPTDASVLVTGESGTGKELLAEAIHRASPRRAKPFVKVNLGGVPTTLF
                     ESEMFGHVKGAFTDARQDRAGRFAAADGGSIFLDEVGELEPAAQVKLLRVLQDRTFEV
                     LGTSATRSVDVRVVSATNRDLGEAVARGAFREDLLYRLNLITLRLPPLRERPDDVPLL
                     AARFLAELSRRHGRPLALAADAGEWLAGRPWPGNVRQLKHLVERSVLLTGKETLGAAD
                     FAFVAGMDEGARREVPAAARTLEELEREAIVRGLARHGGNLSRAAEELGLSRGALYRR
                     LEKYGITP"
     gene            complement(1794..3008)
                     /locus_tag="P5164_11575"
     CDS             complement(1794..3008)
                     /locus_tag="P5164_11575"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY44573.1"
                     /translation="MTRHLLKLVWNRRRYHLLVGLEILSSFLIVFVVAVLAVKTVGNA
                     ARPAGFASDDVTVVEVGLRKRGGGPVADDGADRERMRRALETLRRLEGVEAAAAAFLV
                     PYGTGRAVNSHTVNGRTIEYDYSDVTDDYLAVTRLKLAAGRWFSREDDGRPYRPVVVT
                     RELAREAFGEEDPIGKDIPEGGVEPGTARRRSRVVGVLEAYRPGSDYAASAPFALFRA
                     DLANPADDVPSRIVVRLRPGTPRTFEETLVRTLEAAVPEWSLTAEPLAVAREAANRRW
                     TVPILAATLVAGFLMLLVALGLTGVLWQNVTRRTREIGLRRATGATARQVAVQVVMEV
                     WIVTALAVLAGCAVALQAPLLVPGGFVRAPDAAAGAAVAALLLFALTGLCALYPAWLA
                     ARVFPSEALRYE"
     gene            complement(3005..4240)
                     /locus_tag="P5164_11580"
     CDS             complement(3005..4240)
                     /locus_tag="P5164_11580"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY44574.1"
                     /translation="MLAHTLKTAVRVLLRRKFFTAVSLFGVALTLVVIVVAAALLDHT
                     IGAYPPETRWPRSLGVFRVSLKSEGWTRSAGAGGRFLETCVKDLPGAEVTTFYSQGDR
                     EVTYRDGERLPLWVKRTDGAFWRALDFRFLEGGPYTEADDAAANPVAVVNDATRRRLF
                     GTGPAVGRTLVLDGRTFTVVGVVEDVPFLRPVAFSDVWLPARTARNDDWRRAWVDDWK
                     AFVVARSAADLPGIREELRRRVAAVDLSGEKPFDRLEAEAETILEAIARLPLGNRPNR
                     ATFFVALLSGLALAFMTLPALNLVNLALSRILERAPEVGVRRAFGATRTRLALQFVTE
                     NLVLALAGGAVGLVLSAGVLAAINASGLIPYSDLSLSPTVFVQGLAATCVFGVLSGAL
                     PALRMARLHPVEALRGGPR"
     gene            complement(4243..4908)
                     /locus_tag="P5164_11585"
     CDS             complement(4243..4908)
                     /locus_tag="P5164_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY44575.1"
                     /translation="MIRLSNVEKVYRTDRIETIALSNVSLEVEAGELVSIMGPSGSGK
                     STLLHVMGLLDAPTRGSVELNGRKVDSLSDGALAKVRNAELGFVFQTFHLVPDLSVLD
                     NVEVPFLYRGGGARERRRKAEAALDRVGLSARTRHFPSQLSGGQQQRVAIARAIVGSP
                     RVLLADEPTGNLDSAMGDEVLALLSELNRADGTTVVMVTHDERQAAKTHRVVRLFDGR
                     PVS"
     gene            complement(4905..6170)
                     /locus_tag="P5164_11590"
     CDS             complement(4905..6170)
                     /locus_tag="P5164_11590"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRY44576.1"
                     /translation="MDRPLDDREIRGRRIRRAWAALVAAAILAGAWLGLRALLRPSLT
                     LARVRTGRVDVGPMAATLSAPGTVVPESERVISSPIDSRILRVLVQPGAAVMAGTPLL
                     ELDTTGPALAAARLRKELRLKEAAAERAALALEGTLSSLESRVEVAGLTLSTWGASLE
                     RNRKLFAEGLVSEELLRQSELDEARTRVEVARLKDEMRLARRSADVERGSLEIELATA
                     RSEAAQAERELALSAARSEADGVVTFVATEEGSAVRKGDVLARVADLTAFRVDALVPD
                     VHAARVAPGQEALVRIDDARLPGTIRRVAPEVREGQVAVSVSLSERAHALLRPSLRVD
                     VEIVTGRKERALRLPRGSFGAAEGGVSVWVLKGDEAVRTAVRLGVSNADRYEVLSGLA
                     DGDEVVLSEMTDYAHLTRVGVKGRKGETR"
     gene            complement(6335..>7248)
                     /locus_tag="P5164_11595"
     CDS             complement(6335..>7248)
                     /locus_tag="P5164_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44577.1"
                     /translation="AAVVANAWTVRAGRSDAAGRGMAGAFAVLVLPFPLVGLAIFLLG
                     RSTGAKAAAAVVAAAPLLLTLALEGAGRISAFGKARAAGARARFREPGARALAEAIEE
                     GRIERVRLLLGSVDPDAPGRGGESLLGFALRKRQVDAALELARRGADPLRGPEGEPSA
                     LRLAAQEPAFVEVLRALLGKGTSPDTVLPDDLDRPTPLVFTAMNANAWPNVRAVVEAG
                     ARLDVVDVNGRTPLARAILWRAWDEARLMVERGAPVTAGLRGPTSLEEAFAATRPPEA
                     GTPERASYDLLLASLAERGFPAPDEAR"
BASE COUNT          980 a         2680 c         2742 g          846 t
ORIGIN      
        1 gcgcggctga cgacgtcgac cttcccgtcg aggtcgagcg tgacgacgcc gaggggcgag
       61 gcctcgagga ccttctcgag gaacgcctgc cgctcctcct ggcgcacccg ctcggcctgg
      121 aggcgcgcga gcatctcgtt gaagagggcg acgagggagt cgagctcggg cgtcccggtg
      181 gcgcgcaggc gcgtgccgag ctcgccggcg cgaaggagct cggcgccgtc gcggagcgcc
      241 tccatcggcg cggcgaggcg gcggacgaga agcgccccgg ccagggcgga gaggacgaac
      301 gcggcctccg cggcgaggag ccatcccggg cgatcgcgca ggagagggag ggcgaggagg
      361 gccagcgcgg cgtgaaggac gaggaggtag aggagcgccc ggaccggcca cctcacgggg
      421 tgatcccgta cttctcgagc cttcggtaga gcgccccgcg cgagaggccg agctcctcgg
      481 cggcccggga gaggtttccc ccgtgccgcg cgaggccgcg gacgatcgcc tcccgctcga
      541 gctcctcgag cgtccgcgcg gcggcgggca cttcccgccg cgccccctcg tccatcccgg
      601 cgacgaacgc gaagtcggcg gcgccgagcg tctccttccc ggtcaggagg acgctccgct
      661 ccacgaggtg cttcagctgg cgcacgttcc cgggccaggg gcgccccgcg agccactcgc
      721 cggcgtcggc cgcgagcgcg agcgggcggc cgtgccgccg cgagagctcc gcgaggaacc
      781 gcgcggcgag gagcggcacg tcgtcggggc gctcccggag cggcgggagg cggagcgtga
      841 tgaggttcag ccggtagagg aggtcctcgc ggaaggctcc gcgcgcgacc gcctccccca
      901 ggtcgcggtt cgtggcggag acgacgcgga cgtcgacgct ccgcgtcgcg ctcgtcccga
      961 ggacctcgaa cgtccggtcc tggaggacgc gcaggagctt cacctgcgcc gccggctcga
     1021 gctcgccgac ctcgtcgagg aagatcgagc cgccgtcggc ggccgcgaaa cggccggcgc
     1081 ggtcctgccg ggcgtccgtg aacgccccct tcacgtgccc gaacatctcg ctctcgaaga
     1141 gcgtcgtcgg gacgccgccg aggttcacct tcacgaacgg cttcgcccgc cgcgggctcg
     1201 cccggtggat cgcctcggcg aggagctcct tgcccgtgcc gctctctccc gtcacgagga
     1261 ccgatgcgtc cgtcggggcg acgcgcgcgg cgagggcgag gagccgcacg agcttcgggt
     1321 cgcgcccggc gagcggcgcg aaggcgggag cagaggcgtc gagctcgtcg cgggacggga
     1381 cgtcggcgtc gcgggcgggc ccgaccccgg agagggcgag cgccgtcgcg acggtcgaga
     1441 ggagccgctc cgcgctccac ggcttcgtca cgaagtcggc cgcgccgccg cgcatcccgc
     1501 ggacggcgag ctcgatggag ccccaggccg tcatcaggac gacggggagc cccgggtgcc
     1561 tctcgcggag cgccgcgagg aacgcgagcc cctcctcgcc cgtcgtcccg cgggcgaagt
     1621 tcatgtcggt caggacgagg tccgccggcg cggcggcgag gcgtgcgagc gcctcctccg
     1681 gcgtcgccgc ggaggcgggg cggtaccccg cctgccggag gaggaggacg agcgaggccg
     1741 cgacggcgga gtcgtcgtcg acgacgagga cggcggcgtc gcccttcacg ccctcactcg
     1801 taccggagcg cctcggacgg gaagacacgc gcggcgagcc acgccgggta gagcgcgcag
     1861 agccccgtca gggcgaagag gaggagggcg gcgacggcgg cccccgcggc ggcgtcgggc
     1921 gcgcggacga agccgcccgg gacgaggagc ggcgcctgga gcgccaccgc gcaacccgcg
     1981 agcaccgcca gcgccgtcac gatccagacc tccatcacca cctgcaccgc cacctggcgc
     2041 gccgtcgccc cggtcgcgcg ccggaggccg atctcccgtg tgcgtcgcgt gacattctgc
     2101 cagagcactc ccgtcagccc gagcgcgacg aggagcatca ggaagcccgc gacgagcgtg
     2161 gcggcgagga tcggaacggt ccagcggcgg ttcgccgcct cacgcgccac ggcgagcggc
     2221 tcggccgtga ggctccactc ggggacggcc gcttccagcg tccggacgag cgtctcctcg
     2281 aaggtgcgcg gcgtcccggg gcggaggcgg acgacgatgc gcgacgggac gtcgtcggcc
     2341 ggattcgcga ggtccgcgcg gaagagggcg aacggcgcgg acgcggcgta gtcgctcccc
     2401 ggacggtagg cctcgaggac gccgacgacg cggctccgcc tccgggccgt cccgggctcg
     2461 actcctcctt ccgggatgtc cttcccgatc gggtcctcct caccgaacgc ctcgcgcgcc
     2521 agctcgcgcg tgacgacgac ggggcggtac gggcggccgt cgtcctcgcg tgagaaccac
     2581 cgccccgcgg ccagcttcag ccgcgtcacc gcgaggtagt cgtccgtcac gtcgctgtag
     2641 tcgtactcga tcgttcggcc gttcaccgtg tggctgttca cggcgcgccc cgtcccgtac
     2701 gggacgagga acgcggcggc ggcggcctcg accccttcga ggcgccggag cgtctcgagg
     2761 gcccggcgca tccgctcccg gtccgcgccg tcgtcggcca ccggcccgcc ccccctcttc
     2821 cggaggccga cctcgacgac ggtcacgtcg tcggaggcga acccggccgg ccgggcggcg
     2881 ttcccgacgg tcttcacggc gaggacggcg acgacgaaga cgatcaggaa ggaggagagg
     2941 atctccaggc cgacgaggag gtgataccgc cggcggttcc agacgagctt caggaggtgg
     3001 cgggtcatcg cgggcctccg cggagggcct cgaccgggtg gaggcgcgcc atccggagcg
     3061 ccgggagggc gcccgagagg accccgaaga cgcaggtggc cgcgaggccc tggacgaaga
     3121 ccgtcgggct cagggagagg tcggagtagg ggatgaggcc gctcgcgttg atcgccgcga
     3181 ggactccggc ggagaggacg aggccgaccg cgccgccggc gagcgcgagg acgaggttct
     3241 cggtgacgaa ctggagcgcg aggcgcgtcc gcgtggcccc gaaggcccgc ctcacgccga
     3301 cctcgggcgc gcgctcgagg atccggctca gcgcgaggtt cacgaggttc agcgcgggga
     3361 gcgtcatgaa cgcgagggcc aggccgctga ggagggcgac gaagaacgtc gcgcggttcg
     3421 ggcggttccc gagcgggagg cgcgcgatcg cctcgaggat cgtctccgcc tccgcctcga
     3481 ggcggtcgaa cggcttctcg cccgagagat ccacggccgc gacacggcgc ctcagctcct
     3541 cccggatgcc ggggaggtcc gccgcgctcc tcgcgacgac gaacgccttc cagtcgtcca
     3601 cccacgcgcg ccgccagtcg tcgtttcgcg cggtccgggc cgggagccag acgtcggaga
     3661 aggcgacggg ccggaggaag gggacgtcct cgacgacgcc gacgaccgtg aacgtccgcc
     3721 cgtcgaggac gagcgtcctc ccgacggccg ggccggtgcc gaagagcctc cgccgggtcg
     3781 cgtcgttgac gacggccacc gggttcgcgg cggcgtcgtc ggcctcggtg tagggccctc
     3841 cctcgaggaa ccggaaatcg agcgcccgcc agaacgcgcc gtcggtccgc ttcacccaga
     3901 gcggcagacg ctcgccgtcc cggtaggtca cctcccggtc cccctgcgag tagaacgtcg
     3961 tcacctccgc gccggggagg tccttcacgc acgtctcgag gaatcggccg cccgcgcctg
     4021 cggagcgcgt ccagccttcg ctcttcagcg agacccggaa gacgccgagg ctgcggggcc
     4081 accgggtctc gggcgggtag gcgccgatgg tgtggtcgag gagggccgcc gcgacgacga
     4141 tcaccacgag cgtcagcgcg acgccgaaca ggctgacggc ggtgaagaac ttccggcgga
     4201 ggaggacgcg gacggcggtc ttcagcgtgt gcgcgagcat cgtcagctca ccgggcgccc
     4261 gtcgaagagg cggacgacgc ggtgcgtctt cgccgcctgc cgctcgtcgt gcgtcaccat
     4321 gacgaccgtc gtcccgtccg cgcggttcag ctccgagagg agcgcgagga cctcgtcgcc
     4381 catcgcgctg tcgaggttcc cggtcggctc gtcggcgagg aggacgcggg gcgagccgac
     4441 gatggcccgc gcgatcgcga cgcgctgctg ctggccgccg gagagctgcg aggggaagtg
     4501 gcgcgtccgg gccgagaggc cgacgcggtc gagcgccgcc tcggccttcc gccgccgctc
     4561 gcgcgcgccc ccgccacggt agaggaacgg cacctcgacg ttgtcgagga cggagaggtc
     4621 cgggacgagg tggaacgtct ggaagacgaa gccgagctcg gcgttccgca ccttcgcgag
     4681 cgccccgtcg gagagggagt cgaccttccg cccgttcagc tccaccgagc cgcgggtcgg
     4741 cgcgtcgagg agccccatca cgtggaggag cgtcgacttc ccggagcccg agggccccat
     4801 gatcgacacg agctccccgg cctccacctc gaggctcacg ttcgacaggg cgatcgtctc
     4861 gatccggtcc gtccggtaga ccttctcgac gttcgaaagt cggatcatcg cgtttccccc
     4921 ttccgtccct tcacgcccac ccgggtcagg tgcgcgtagt ccgtcatctc ggagaggacc
     4981 acctcgtccc cgtccgcgag gcccgagagg acctcgtatc gatcggcgtt cgagaccccg
     5041 agccgcactg cggtccgcac cgcctcgtct cccttcagga cccagaccga gacgcccccc
     5101 tcggccgctc cgaacgaccc gcgcggcagg cgcagcgccc gctccttccg cccggtgacg
     5161 atctccacgt cgacgcgcag gctcggccgg aggagcgcgt gcgccctttc ggagagggag
     5221 acgctcacgg cgacctgccc ctcccgcacc tcgggcgcca cgcgccggat cgtccccggc
     5281 aggcgcgcgt cgtcgatccg cacgagagcc tcctgcccgg gcgccacgcg cgccgcgtgg
     5341 acgtcgggga cgagcgcgtc gacgcggaac gccgtgaggt cggccacccg cgcgaggacg
     5401 tcccccttcc gcacggcgga accctcctcc gtcgcgacga acgtcacgac gccgtccgcc
     5461 tcgctccggg cagcggagag agcgagctcg cgctcggcct gggccgcctc gctccgcgcc
     5521 gtcgcgagct cgatctcgag ggagcctctc tccacgtcgg cggagcgccg ggcgaggcgc
     5581 atctcgtcct tcaggcgggc gacctccacg cgcgtccgcg cctcgtcgag ctcggactgg
     5641 cggaggagct cctcggagac gagcccctcg gcgaagagct tccggttccg ctcgaggctc
     5701 gcgccccagg tcgagagggt caggccggcg acctcgaccc ggctctcgag cgaagagagc
     5761 gtcccctcga gcgcgagcgc ggcgcgctcg gccgccgcct ccttcagccg gagctccttg
     5821 cggaggcggg ccgccgcgag ggccggccct gtcgtgtcga gctcgagcag gggcgtcccc
     5881 gccatcacgg cggcgccggg ctggacgagg acgcggagga tccgggagtc gatcgggctc
     5941 gagatcacac gctcggattc cgggacgacc gtccccggcg ccgagagagt cgcggccatc
     6001 ggcccgacgt cgacccttcc ggtcctcacc cgggcgagcg tgagcgacgg tcggaggaga
     6061 gcgcggaggc cgagccacgc cccggcgagg attgctgcgg cgacgagagc cgcccacgcc
     6121 ctccggattc gcctgccgcg gatttcacgg tcgtcgagcg gtcgatccac gccgtgccgg
     6181 agcgcaagcg gggtgccagc tcacgcggcg tcgccaactc attcgcggag aaacgtatgc
     6241 gtcgcgcgga gcggagcggg gccgccgacg gagatgtccg tcgccggacg tcggtgtccg
     6301 gaaccggacg atctggcgtg ccggcgtccc gacttcagcg cgcttcgtcg ggggcgggga
     6361 atccgcgttc ggcgagcgag gcgaggagga ggtcgtaaga ggcgcgctcg ggggtgcccg
     6421 cctcgggagg tcgcgtcgcc gcgaacgcct cctcgaggct cgtcgggccg cgcagccccg
     6481 ccgtcaccgg agcgccgcgc tcgaccatga gccgcgcctc gtcccacgcc cgccagagga
     6541 tcgcgcgcgc gagcggcgtc cggccgttga cgtcgacgac gtcgagccgg gcccccgctt
     6601 cgacgacggc cctcacgttc ggccaggcat tcgcgttcat cgccgtgaag acgagcggtg
     6661 tcgggcggtc gaggtcgtcc ggcaggaccg tgtcggggct cgtgcccttc ccgaggaggg
     6721 cgcgcaggac ctcgacgaag gcgggctcct gcgccgcgag gcggagggcg ctcggctcac
     6781 cctccggccc gcgcaacggg tcggcgcccc gtcgcgcgag ctcgagcgcg gcgtcgacct
     6841 gccgtttccg gagcgcgaag ccgaggagcg actcgccgcc cctccccggc gcgtccgggt
     6901 cgacgctccc gaggagtaat cggacccgct cgatccgtcc ctcctcgatc gcttccgcga
     6961 gcgcccgcgc cccgggctcg cggaagcgcg cccgggcgcc tgcggcgcgg gccttcccga
     7021 aggcggagat ccggccggcc ccctcgagcg cgagcgtgag gaggagcggc gcggccgcga
     7081 ccaccgccgc ggcggccttc gcgcccgtcg atcggccgag gaggaagatc gcgagcccga
     7141 ccagcgggaa cggcaggacg agcaccgcga acgccccggc cattccgcgg cccgccgcgt
     7201 cggagcggcc ggcccggacg gtccacgcgt tcgcgacgac ggcggcca
//
LOCUS       DAOTEZ010000206         7248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_7335_length_7248_cov_4.608508, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000206 DAOTEZ010000000
VERSION     DAOTEZ010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7335_length_7248_cov_4.608508"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..596)
                     /locus_tag="P5164_11600"
     CDS             complement(54..596)
                     /locus_tag="P5164_11600"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HRY44578.1"
                     /translation="MIELLRRRLVRPILEQLTQGLSPDAIALTIAIGLAIAVIPVVGT
                     TTILCTAAAIVLRLNQPLIQTINYLSFPLQLAFILPYLKLGRILFGGPVIRMSAEELA
                     AFVTSRPGEAFEVLWRVTLQAVGAWALTAPLIAAAVYFAVRPVLRGAARRLRSARTAA
                     TRCLAATGRISSTAVPATIR"
     gene            complement(593..1882)
                     /locus_tag="P5164_11605"
     CDS             complement(593..1882)
                     /locus_tag="P5164_11605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HRY44579.1"
                     /translation="MTTIESASDALVGTAPAPGLLDRLAARLALRALEGLREGAVLLT
                     LPDGTTGRFGRDDARPVRLVARSWRPFRALLLAGDLGAAEAYLDGEWTTDDLPGLVRL
                     FARNADLLDRETWTTRLANAANRLLHARNRNSRAGSRRNVRAHYDLGNDLYETFLDPS
                     MTYSCALFETGDETLEEAQEKKLRRIAEKARIRPGDHVLEIGCGWGSFALLAAREHGA
                     RVTGVTLSTEQAARARERVAREGLSDRVEIRLVDYRDLPAEGRTYDRVVSIEMLEAVG
                     EEFLPGYFEVVDRLLAPEGLAVIQSITVPDDRYARYRRRPDFIQKHVFPGSHCPSVGA
                     IVAAVSARSRLLVHHLEDVGAHYAETLRRWREAFRANVPRVRRLGYDERFTRLWDFYL
                     AYCEGGFAERHIGDVQVVLTRSGNRALGPAPGYRGEA"
     gene            complement(1879..2676)
                     /locus_tag="P5164_11610"
     CDS             complement(1879..2676)
                     /locus_tag="P5164_11610"
                     /inference="COORDINATES: protein motif:HMM:NF018770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HRY44580.1"
                     /translation="MTGPETKAPLASALYVGTVRHRRLSPKPHAFSYPVYQLLLDLDE
                     LPRVEEEVGLFRHNRNGWSALFDRDYLGPAERPIKEKLRDWLAARGHVLGERRVLLLT
                     HARVLGYAFNPVSYYYVLGADGRLDLAVAEINNTFGETFGYVLERRGAETGISTPRFP
                     KVFHVSPFLPMDLEYEFHLSVPGESLAVHVDDFAGGVKVFDATLSARRRPLTTASLVW
                     ALLSSPLMPARVIAWIHWQALKLWLKETPVYTRPEPPAGLIHTRRAA"
     gene            complement(2673..3929)
                     /locus_tag="P5164_11615"
     CDS             complement(2673..3929)
                     /locus_tag="P5164_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY44581.1"
                     /translation="MKLAIVGTGISGLGAAWRLHGRHDLRVFEREGRAGGHSHTVAVP
                     DGGREVPVDTGFLVYNEVTYPNLVKLFKMLGVPTKESDMSFSVRCERCDLEWCGTGLS
                     GVFAQPANLLRPSFHGMLLEIARFNREAPRLLDEPGAETLTLAAYLDRERYGRAFRSH
                     YLIPMAASVWSSGPAAMESFPAATLVRFFRNHGFLGVTTQLRWRTVDGGSREYVKLLT
                     EPFRDRIHLGNGVTKVRRDGHGVELVFADGGSQRFDGVLIATHADEALAMLEEPTPDE
                     RRLLGAWSFSRNETLLHSDPSFLPRRRGARASWNYHLDDCSRPWGSATLHYLLNRLQG
                     LPTETDWIVSLNPRREPAEGTVARRLLYTHPVYTPASVATQAELPTLNGKGRTWYAGA
                     WFGYGFHEDGLRSGLEAAVSLGAEPL"
     gene            4106..5404
                     /locus_tag="P5164_11620"
     CDS             4106..5404
                     /locus_tag="P5164_11620"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRY44582.1"
                     /translation="MRTEGETRPAASSDPERIDLNEVVLALSDALDLVGSHVVQHGKR
                     VAFMAASCGRSLALEESERSDLLLAAVLHDCGVSSTRLHARLLEADLAFEEARGHAEA
                     GAELLRRFEPLSRVAAIVGLHHTPWDSGERRAADPRVALLANLVFLADRVDALLQARR
                     GSDPLLATDELRRWVFVRTGSLFSEEAVGAFLATSAAEAFWLTLETHHLERALRDEMR
                     LEQSRSVPASALEDLALVFAEIVDAKSRFTAAHSVGVARLAALLGRLAGFDPRTSSHL
                     RVAGLLHDLGKLRVPDEVLDKEAPLDEGEFAKIERHAFETFQILRRVEPLREIATWAG
                     YHHEGLSGKGYPFHRRAEEIPLPARLLAIADIFQALAQRRPYRGPLGPEAILDHLRRF
                     VEAGKLDPRGVELVAANLAACYRAATLDGDAGRGERTSPA"
     gene            5445..6149
                     /gene="queC"
                     /locus_tag="P5164_11625"
     CDS             5445..6149
                     /gene="queC"
                     /locus_tag="P5164_11625"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HRY44583.1"
                     /translation="MTSRPPAVVLLSGGLDSATCLAIARSEGFAPYALSFRYGQRHAP
                     ELAAAGRVAAALGAERHVVASIDLGLFGGSALTADIDVPKDRDGEEIARGIPITYVPA
                     RNAVFLTFALAWAEVLGAFDLFVGVNALDYSGYPDCRPEFVSAFEAMANLATRAGVEG
                     AGRFRVRAPLLSMTKAEIIRTGLALGVDYGLTLSCYDPSTEGAPCLRCDACHLRARGF
                     RDAGVPDPALQQAGRT"
     gene            6146..6787
                     /gene="queE"
                     /locus_tag="P5164_11630"
     CDS             6146..6787
                     /gene="queE"
                     /locus_tag="P5164_11630"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HRY44584.1"
                     /translation="MSYAVKEVYYTLQGEGLQTGRAAVFLRFAGCNLWSGREEGRASA
                     TCRFCDTDFVGVDGPGGGRFGDAASLAAAVRGAWEAPLPERGRPLVVCTGGEPLLQLD
                     AALVEALHAEGFEVAVETNGTRTPPPGLDWITVSPKAGTELAVKAGDELKLVFPQSGA
                     EPELYEQLAFRAFVLQPMDGATRDRNVAAAAAYCLAHPLWRLGLQSHKLVGLR"
BASE COUNT         1049 a         2629 c         2572 g          998 t
ORIGIN      
        1 gtcgaggccg attgcgccgg aaaagatgaa gcggttggcg ccgccgccgc cggtcagcgt
       61 atcgttgccg gcaccgccgt cgagctgatc cttcccgtcg ccgccaagca gcgtgtcgcc
      121 gccgtcctcg ccgaacggag ccggcgcgcg gctccccgca ggacgggccg tacggcgaag
      181 tagaccgcgg ctgcaatcag gggagcggtc agcgcccagg cgccgaccgc ctgcagcgtc
      241 acccgccaga gcacctcgaa cgcctccccg ggccgcgacg tcacgaacgc cgcgagctcc
      301 tctgccgaca tccggatgac cggcccgccg aagaggatcc gcccgagctt caggtacggg
      361 aggatgaacg cgagctggag cgggaacgag aggtagttga tcgtctggat gagcggctgg
      421 ttcagccgga ggacgatcgc cgcggcggtg cagaggatcg tcgtcgtccc gacgacgggg
      481 atcacggcga tcgcgagacc gatcgcgatc gtgagggcga tcgcgtccgg ggagagcccc
      541 tgcgtcagct gctcgagaat cgggcggacg agccgcctcc gcaggagctc gatcacgcct
      601 ctccccggta gccgggcgcg ggaccgaggg cgcggttccc ggatcgggtc aggacgacct
      661 ggacgtcccc gatgtgacgc tcggcgaagc ctccctcgca gtaggccagg tagaagtccc
      721 agaggcgcgt gaagcgctcg tcgtagccga gccgcctcac ccgcgggacg ttcgcccgga
      781 acgcctcgcg ccaccgccgg agggtctcgg cgtaatgcgc cccgacgtcc tcgaggtggt
      841 ggacgaggag gcgcgagcgg gccgagaccg ctgcgacgat cgcgccgacc gaggggcagt
      901 ggctccccgg gaagacgtgc ttctggatga agtcggggcg gcggcggtag cgggcgtagc
      961 ggtcgtcggg aacggtgatc gactggatca ccgcgagccc ctcgggcgcg aggagacggt
     1021 cgacgacctc gaagtagccc gggaggaact cctcccctac cgcctcgagc atctcgatcg
     1081 acacgacccg atcgtacgtc cttccctcgg ccgggaggtc gcggtagtcg acgaggcgga
     1141 tctcgacgcg gtccgacagc ccctcccgcg ccacccgctc ccgcgcccgg gccgcctgct
     1201 cggtggagag ggtcaccccg gtgacgcgcg cgccgtgctc ccgcgcggcg aggagggcga
     1261 acgagcccca cccgcagccg atctcgagga cgtggtcgcc gggccggatc cgggccttct
     1321 ccgcgatcct ccggagcttc ttctcctgcg cctcctcgag cgtctcgtcc cccgtctcga
     1381 agagcgcgca ggagtaggtc atcgaggggt cgagaaaggt ctcgtaaagg tcgttgccga
     1441 ggtcgtagtg cgcccggacg ttccgccgcg agccggcccg gctgttccgg ttcctcgcgt
     1501 ggaggagccg gttcgcggcg ttcgcgaggc gcgtcgtcca cgtctcgcgg tcgaggaggt
     1561 cggcgttccg cgcgaagagc cgcacgaggc ccggcaggtc gtccgtcgtc cactcgccgt
     1621 cgaggtaggc ctccgcggcg ccgaggtcgc cggccaggag gagcgcacga aacggacgcc
     1681 aggaccgggc gacgagccgg acggggcggg cgtcgtcgcg cccgaagcgg cctgtcgtcc
     1741 cgtcggggag cgtcaggagg acagctccct cccggaggcc ctcgagggcg cgaagggcga
     1801 gccgcgcggc gagccggtcg aggagccccg gcgcgggggc ggtgccgacg agggcgtcgg
     1861 aggcagattc gatcgtggtc acgcggctct cctcgtgtgg atcagcccgg ccgggggctc
     1921 ggggcgggtg tagacggggg tctccttcag ccagagcttc agcgcctgcc agtggatcca
     1981 ggcgatcacc ctcgccggca tcagggggct cgaaaggagc gcccacacga ggctcgccgt
     2041 cgtgagcggc cgcctccgag ccgagagcgt cgcatcgaag accttgacgc cccccgcgaa
     2101 gtcgtcgacg tgaacggcga ggctctctcc cggcaccgag aggtggaact cgtactcgag
     2161 gtccatcggg aggaacggcg agacgtggaa gaccttcggg aagcgcggcg tcgagatccc
     2221 cgtctcggcc ccgcggcgtt ccaggacgta gccgaacgtc tcgccgaagg tgttgttgat
     2281 ctcggcgacg gcgaggtcga gccgcccgtc cgcaccgagg acgtagtagt agctcaccgg
     2341 gttgaaggcg tagccgagga cgcgggcgtg ggtcaggagg aggacgcgcc gttcgcccag
     2401 gacgtgtccg cgcgcggcga gccagtcgcg gagcttctcc ttgatcggcc tctccgccgg
     2461 cccgaggtag tcgcggtcga acagggcgct ccacccgttc cggttgtgcc ggaagaggcc
     2521 cacctcctcc tcgacccgcg ggagctcgtc gaggtcgagg aggagctggt agaccggata
     2581 cgagaaggcg tggggcttcg gcgagaggcg ccggtggcgg accgtcccga cgtagagggc
     2641 cgaggcgagc ggcgccttcg tctccggtcc ggtcacagcg gctccgcacc gagcgaaacg
     2701 gcggcctcga ggcccgaccg cagcccgtcc tcgtggaagc cgtagccgaa ccaggcgccg
     2761 gcataccagg tccgtccctt cccgttcagc gtcggcagct cggcctgcgt cgcgacgctc
     2821 gccggcgtgt agaccgggtg ggtgtagagg agccgccggg cgaccgtccc ctccgccggc
     2881 tcgcgccgcg ggttgagcga gacgatccag tccgtctccg tcggcagacc ctggagccgg
     2941 ttgaggaggt agtggagcgt cgccgagccc cagggacgcg agcagtcgtc gaggtgatag
     3001 ttccaggagg cgcgggctcc ccggcggcgc gggaggaacg aggggtcgga gtggaggagc
     3061 gtctcgttcc gcgagaagct ccaggcgccg aggaggcgcc gctcgtccgg cgtcggctcc
     3121 tcgagcatcg cgagcgcctc gtcggcgtgc gtcgcgatca ggacgccgtc gaaccgctgg
     3181 ctcccgccgt ccgcgaagac gagctcgacg ccgtgcccgt cgcgccgcac cttcgtcacc
     3241 ccgttcccga ggtggatccg gtcgcggaag ggctccgtca ggagcttcac gtactcgcgg
     3301 ctcccgccgt cgacggtccg ccagcggagc tgcgtcgtca ccccgaggaa gccgtggttc
     3361 cggaagaacc tcacgagcgt cgccgccggg aacgactcca tcgcggcggg gcccgacgac
     3421 cagaccgacg cggccatcgg gatcaggtag tggctccgga acgcccggcc gtaccgctcg
     3481 cgatcgaggt acgcggcgag agtgagcgtc tcggcgccgg gctcgtcgag gagccgcggc
     3541 gcctcgcggt tgaagcgggc gatctcgagc agcatcccgt ggaacgacgg gcggaggagg
     3601 ttcgccggct gggcgaagac gcccgaaagc cccgtcccgc accactcgag gtcgcacctc
     3661 tcgcaccgga cggagaacga catgtcgctc tccttcgtcg ggacgccgag catcttgaag
     3721 agcttcacga ggttcgggta ggtcacctcg ttgtagacga ggaagccggt gtcgacgggg
     3781 acctcgcgtc ccccgtccgg gaccgcgacg gtgtggctgt ggcctccggc ccgcccttcc
     3841 ctctcgaaga cccgcaggtc gtgcctcccg tggagccgcc aggccgcccc gaggcccgag
     3901 atacccgttc ctacgatcgc gagcttcatc cccccgtcgt cctccgcagc cctcctacga
     3961 aacggatcgg cggatgggat tgcgaccggg agagggcggc gagtgtcacg atccgccccg
     4021 gcgtacgatt cccgggcagc tcgaaaccgg cgccgggaga cggccgatcg gaccccggcc
     4081 tcgcggggtc ccgaagggag cgaagatgag gacggaaggc gagacccggc cggccgccag
     4141 ctccgacccc gagcggatcg acctgaacga ggtcgtcctc gccctctccg acgccctcga
     4201 cctcgtcggg tcgcacgtcg tccagcacgg gaagcgggtc gcgttcatgg cggcctcctg
     4261 cggccggtct ctcgcgctcg aggagagcga gagaagcgac ctcctcctcg cagcggtcct
     4321 ccacgactgc ggcgtctcct cgacgcggct ccacgcgagg ctcctcgagg ccgacctcgc
     4381 gttcgaggag gcccgcggcc acgcggaggc cggcgcggag ctcctccgcc ggttcgagcc
     4441 tctttcgcgt gtcgcggcga tcgtcgggct ccaccacacc ccgtgggact ccggggagcg
     4501 gcgcgccgcc gacccgcgcg tcgccctcct cgcgaacctc gtcttcctcg ccgaccgcgt
     4561 cgacgctctc ctccaggccc gtcgaggctc cgaccccctt ctcgcaacgg acgagctgcg
     4621 ccgctgggtc ttcgtccgga ccgggagcct cttctcagag gaggccgtcg gcgcgttcct
     4681 cgcgacctcg gcggcagagg ccttctggct gaccctcgag acgcatcacc tcgagagggc
     4741 cctgcgagac gagatgcgcc tcgagcagag ccgctccgta cccgcctccg ccctcgagga
     4801 cctcgccctc gtcttcgcgg agatcgtcga cgcgaagagc cgcttcacgg ccgcccactc
     4861 cgtcggcgtc gcacgtctcg ccgccctgct cgggcggctt gccggcttcg acccgcggac
     4921 ctcctcccac ctccgggtcg ccggcctcct gcacgacctc ggcaagctcc gggtccccga
     4981 cgaggtcctc gacaaggagg caccgctcga cgagggggag ttcgcgaaga tcgagcgaca
     5041 cgcgttcgag acgttccaga tcctccgccg ggtcgagccg ctccgcgaga tcgccacgtg
     5101 ggccgggtac caccacgagg ggctctcggg aaagggctac ccgttccacc ggcgcgcgga
     5161 ggaaatcccg cttcccgcgc ggctcctcgc gatcgcggac atcttccagg ctctcgcgca
     5221 acggcgcccc taccgcggtc cgctcggccc ggaggcgatc ctcgaccacc ttcgccgatt
     5281 cgtcgaggcg ggaaagctcg acccgcgcgg cgtcgagctc gtggccgcga acctcgccgc
     5341 gtgctaccgg gccgccacgc tcgacgggga cgcgggacgc ggcgaacgga cttcgcccgc
     5401 ctgacctcgc gcccggcccc cggacggcgt ggtatgaagc cgccgtgacg tctcgccccc
     5461 ccgccgtcgt cctcctctcg ggcggcctcg attcggccac ctgcctcgcc atcgccagga
     5521 gcgaagggtt cgcgccctac gctctcagct tccgctacgg acaaaggcat gcccccgagc
     5581 tcgccgcggc cggacgcgtt gccgcggcgc tcggcgccga gcgtcacgtc gtcgcgtcga
     5641 tcgacctcgg ccttttcggc ggctcggccc tcaccgccga catcgacgtc ccgaaggacc
     5701 gcgacggcga agagatcgcc cgcggcatcc cgatcaccta cgtcccggcg cgcaacgcgg
     5761 tgttcctcac cttcgccctc gcctgggccg aggtcctcgg cgcattcgac ctgttcgtcg
     5821 gggtgaacgc cctcgactac agcggctacc ccgactgccg cccggaattc gtctcggcgt
     5881 tcgaagcgat ggcgaacctc gcgacgcggg ccggcgtcga gggggccggc cggtttcgcg
     5941 tccgcgcacc gctcctctcg atgacgaagg ccgagatcat ccgaacaggt ctcgccctcg
     6001 gcgtcgacta cggcctcacg ctcagctgct acgacccttc gacggagggc gcgccgtgcc
     6061 tccggtgcga cgcctgccac ctccgggcga ggggcttccg ggacgcgggc gtccccgacc
     6121 cggccctcca gcaagcgggc cgcacgtgag ctacgccgtc aaggaggtct actacacgct
     6181 ccagggcgaa gggctccaga cggggcgtgc cgccgtcttc ctccgcttcg cgggctgcaa
     6241 cctctggtcg ggccgggagg agggtcgcgc gtccgccacg tgccgcttct gcgacaccga
     6301 cttcgtcggc gtcgacggcc ccggcggagg ccgcttcggc gatgcggcct ccctggcggc
     6361 agcggtccgc ggggcctggg aggcccctct tcccgagcgc ggccggccgc tcgtcgtctg
     6421 cacgggaggc gagcccctcc tccagctcga cgcggcgctc gtcgaggcgc tccacgccga
     6481 aggattcgag gtcgcggtcg agacgaacgg cacccggacg cccccgccgg gcctcgactg
     6541 gatcacggtc agcccgaagg ccgggaccga gctcgccgtg aaggcgggcg acgagctgaa
     6601 gctcgtcttc ccgcagtccg gcgccgagcc ggagctctac gagcagctcg ccttccgggc
     6661 gttcgtcctg cagccgatgg acggcgcaac gagggatcgg aacgtcgcgg cggccgccgc
     6721 gtactgcctt gcccacccgc tctggcgcct cggccttcag agccacaagc tcgtcggcct
     6781 gcgctgaatt ccggctcccc gatcgagcgg aggatgcgaa aagaagcccc gcgcttccga
     6841 ccgggtccag gtgccgcgca gcgagggcga ggagagcgag ggcgagcgcg gcaacggact
     6901 cgcgggtcct ccacgaaggg cttcagccgc tagttacggc gcgaccggac aaaggttccg
     6961 aggctcccgt gcggcatcct gcggcctgcg gcggccccgc tgcgaaattc aacacggcac
     7021 gacaccagcg tcatcgaacc gatgtacccg gatcaagcca tttcgatgca gacacttgtg
     7081 cgatccgacc ggcagtgcgt ccgcgtagag gagttcagca ccgcctgaac ggcccatcga
     7141 gcgaagtccg cgctcaagtc gttgacacaa ttatgcttag cgcgagttcg agaggcgtgg
     7201 cagcgccctt gcgatatctc cgggcgaagc gggccgagag aaatcggg
//
LOCUS       DAOTEZ010000207         7243 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_7341_length_7243_cov_5.470228, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000207 DAOTEZ010000000
VERSION     DAOTEZ010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7243
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7341_length_7243_cov_5.470228"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="P5164_11635"
     CDS             complement(<1..1034)
                     /locus_tag="P5164_11635"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRY44585.1"
                     /translation="MAAVLPVETESLPSRPEAERSLLGSLLIDGGLLTRVMEHVIPED
                     FASEPHRLVYEACLALSDRKEAVDLVTVQAELERHGRLERCGGDAYLAGLLDNVPDVE
                     SVESYARLVREASLRRQLILQSRRAARAAVEAPDAQAVLEVAQQELVDIAKGSLRGGF
                     VRLSDIAEKNEEEIQKIQARGSMLTGLPTGFAKLNALTQGMQRTDLIVLAARPGMGKT
                     AFALNIASYLTIHSGYCVAFFSLEMSAPQLGFRIQCAEARVNLQRLREGRLSKEEKKY
                     LFYTTQQMRDARFFIDDQPSISLLEMRARALQLKQAQKGLDAIFVDYLQIMGSRQKYE
                     NRTQEVGAYSR"
     gene            complement(1072..1977)
                     /locus_tag="P5164_11640"
     CDS             complement(1072..1977)
                     /locus_tag="P5164_11640"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRY44586.1"
                     /translation="MSALAGVGTGATRAAFGGADLHVHSTASDGVESPETLAALAAAA
                     GLSVAVLTDHDAVHGVAAFVAAARGTGLVAAGGAELSVDEAGQDVHLLGLFVDPDEPR
                     LGSRLAELREVRERRAERMVERLAALGIVLDLERLRAEVGDGAFGRPHVARALVAAGA
                     VASVAEAFERYLGDGAPAFVPKAKWSLGEAIASVRAAGGLAVLAHPVWYADVPGLLRR
                     GRELGLDGVEVFHPDNEGREEELLSEADGLGLLVSGGSDFHSPSYGCPVGGCRVGWPL
                     WERLAEAAVARRRDAARPALDLAPR"
     gene            complement(1974..3416)
                     /gene="pyk"
                     /locus_tag="P5164_11645"
     CDS             complement(1974..3416)
                     /gene="pyk"
                     /locus_tag="P5164_11645"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRY44587.1"
                     /translation="MAKPIREPRATKIVATIGPRAETGGELVRLLSAGVDVVRLNGAH
                     CRPGDIARRVALVRSAEKALGRPVGVLLDLGGPKIRVGPIEGDTTAWEAGDAVEIVPG
                     HAMGSGRTISVTYPALLSEVAPGAEIRIADGLIRLKVERRERGALRARVLAGGTVRKG
                     AGVNLPSSALSAPAVTPKDRRDLREGLEAGIQFVGLSFVRSVDHVKALRRLLAAAPAE
                     RRPWIVSKIERVEAVRALREIAAASDVLMVARGDLGVEIGLAAVPGVQRAILAVGREK
                     AVPVIVATQMLESMVENPVPTRAEVSDVAGAVHDGADAVMLSGETAVGAHPIEAVRTM
                     DEIARVAEAQRPVAIEATERSATGADHTPVVCDMAVVAARRAGARAMVVYTASGRTAR
                     LLSKEGTRLPIVAITSSEEVRRRLTILRNVVSYRIPASSSVEEMIASGDAVLRAHGLA
                     GATVVEVSGAAALEGATNTVRIRTLRPAAR"
     gene            3449..4081
                     /gene="folE"
                     /locus_tag="P5164_11650"
     CDS             3449..4081
                     /gene="folE"
                     /locus_tag="P5164_11650"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRY44588.1"
                     /translation="MAEIPESHTGSHLVLHDAPAPTDPRIDQIAGHVREIIRILGLSP
                     EADHNLRDTDRRVAKMYLEIFSGLEEGNFPDLTTFPNEEGYQAMVIEKEIPFYSMCAH
                     HFVPFYGHGHIAYIPQERIVGLSKLPRILEFFARRPQLQERLTEQVAGVLWEELRPLG
                     VMVVIEARHLCVEMRGVKKPGSVTVTSALRGVFKQKTVREEFLDLLRRRG"
     gene            complement(4125..4862)
                     /locus_tag="P5164_11655"
     CDS             complement(4125..4862)
                     /locus_tag="P5164_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44589.1"
                     /translation="MKAGALSAALVGILAVLAGSAAGGEKAAGSWVVVQSDGARVVFD
                     SPPVRRGTRLVGRLLSGGALVSLPLARIDEAATRAANEPGAPKPTPPPAATPAPGPFE
                     TPPLGNLVKLRTSAEEARAHIEGARKGTPAPPRPAREVPPVEVAEPTDLLGRGERYWR
                     ERADERREALEAAAADRQIAERTLAEAERAWLGLSEAETTTYVLYLLEARERAERARQ
                     RHLGEQQRWEELGEEARKAGAFPGWLR"
     gene            complement(4859..5596)
                     /locus_tag="P5164_11660"
     CDS             complement(4859..5596)
                     /locus_tag="P5164_11660"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HRY44590.1"
                     /translation="MRIVSLCPSTTESLVALGLGGSLAGVTRYCVHPREALEGIPRVG
                     GTKDPDHAAIGALAPDLVLCNAEENREADVAALAARHRVDVSHPTRVADVPPLLRRLG
                     ALTGSEPAAEGWARRVEERLAAARPAARPVRFAYLIWKGPWMAAAAGTYIADLLETFG
                     GVDVFARERGPWPRTDEAELAALAPGLVVLPDEPYRFGEKERALFRGLLPESRVELVS
                     GDDFCWHGVRTLRGLEAAGALLRGSVP"
     gene            complement(5596..7019)
                     /locus_tag="P5164_11665"
                     /pseudo
     CDS             complement(5596..7019)
                     /locus_tag="P5164_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011358003.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
BASE COUNT          948 a         2748 c         2558 g          989 t
ORIGIN      
        1 cgcgagtagg cgccgacctc ctgcgtccgg ttctcgtact tctggcgcga gcccatgatc
       61 tggaggtagt cgacgaagat cgcgtcgagg cccttctgcg cctgcttgag ctgcagcgcg
      121 cgcgcgcgca tctcgaggag cgagatcgac ggctggtcgt cgatgaagaa gcgggcgtcg
      181 cgcatctgct gcgtcgtgta gaagaggtac ttcttctcct ccttcgagag ccgcccctcg
      241 cggaggcgct ggaggttcac gcgcgcctcg gcgcactgga tccggaagcc cagctgcggg
      301 gcgctcatct cgagcgagaa gaaggcgacg cagtagccgg agtggatcgt caggtacgac
      361 gcgatgttca gcgcgaaggc cgtcttcccc atgcccggcc gggccgccag gacgatcagg
      421 tcggtcctct gcatcccctg ggtcagggcg ttcagctttg cgaagccggt cgggaggccg
      481 gtcagcatcg agccgcgcgc ctggatcttc tggatctcct cctcgttctt ctccgcgatg
      541 tccgacagcc gcacgaagcc gccgcggagg ctccccttcg cgatgtcgac gagctcctgc
      601 tgcgcgacct cgaggacggc ctgcgcgtcg ggggcctcga cggcggcccg tgccgcgcgg
      661 cgcgactgga ggatgagctg ccggcggagc gacgcctccc tcacgaggcg cgcgtagctc
      721 tccacgctct cgacgtcggg gacgttgtcg aggaggcccg cgaggtaggc gtctccgccg
      781 cagcgctcca gccggccgtg cctctcgagc tcggcctgga cggtgacgag gtcgaccgcc
      841 tccttccggt ccgagagcgc gaggcacgcc tcgtagacga ggcggtgcgg ctccgaggcg
      901 aagtcctccg ggatcacgtg ctccatgacc cgcgtgagca gcccgccgtc gatcaggagg
      961 gagccgagca ggctccgctc ggcttccggg cgcgacggga gggactcggt ctcgacgggg
     1021 aggacggccg ccatggatcg cctgttctac accatccggg cgggaaccgt ctcagcgcgg
     1081 cgcgaggtcg agcgcgggcc gcgccgcgtc gcgcctccgc gcgaccgccg cctccgcgag
     1141 ccgctcccag agcggccatc cgaccctgca gccgccgacg gggcatccgt aggacggaga
     1201 gtggaagtcg gacccgcccg agacgaggag cccgagcccg tccgcctcgg aaagcagctc
     1261 ctcctcgcgc ccctcgttgt cggggtggaa gacctccacg ccgtcgagcc cgagctcgcg
     1321 ccctctccgg agcaggccgg gaacgtccgc gtaccagacc gggtgcgcga ggacggcgag
     1381 gccgcccgcc gcgcggaccg acgcgatcgc ctccccgagg ctccacttcg ccttcgggac
     1441 gaacgccggc gcgccgtccc ctagatacct ctcgaacgcc tccgcgacgc tggcgaccgc
     1501 gcccgccgcg acgagggcgc gcgccacgtg cggccggccg aacgcgccgt cgccgacctc
     1561 cgcccgcagc cgctccaggt cgaggacgat cccgagcgcc gcgagccgtt cgaccatccg
     1621 ctccgcccgc ctctcccgga cctcccgaag ctcggcgaga cgcgagccga ggcgcggctc
     1681 gtccgggtcg acgaagaggc cgaggaggtg gacgtcctgt cccgcctcgt cgaccgagag
     1741 ctccgctccc ccggccgcga cgagcccggt cccgcgggcc gcggcgacga acgccgccac
     1801 gccgtggacg gcgtcgtggt cggtcaggac cgcgaccgag aggccggcgg ccgcggcgag
     1861 cgccgcgagc gtctccgggc tctcgacgcc gtcggaagcc gtcgagtgaa cgtgaaggtc
     1921 ggccccgccg aaggcggccc gcgtcgcccc ggtcccgacg ccggccagag cgctcatcgt
     1981 gccgcgggcc gcagcgtccg gatccggacg gtgttcgtcg ccccttccag cgccgcggcg
     2041 ccggagacct cgacgacggt cgctccggcg aggccgtgcg cccggaggac ggcgtccccg
     2101 ctcgcgatca tctcctccac ggaggaggag gccggaatcc ggtaggagac gacgttccgg
     2161 aggatcgtga gcctccggcg cacctcctcg gagctcgtga tcgcgacgat cggcaggcgg
     2221 gtcccctcct tcgagaggag gcgcgcggtc cgtcccgacg ccgtgtagac gaccatcgcg
     2281 cgggcccccg cgcgccgggc cgcgacgacc gccatgtcgc agacgacggg cgtgtggtcc
     2341 gcgcccgtcg cgctccgctc cgtcgcctcg atcgcgacgg ggcgctgggc ctccgcgacg
     2401 cgggcgatct cgtccatcgt cctgaccgcc tcgatcggat gggccccgac cgcggtctcg
     2461 cccgagagca tcaccgcgtc ggcgccgtcg tggaccgcgc ctgcgacgtc ggagacctcc
     2521 gcccgcgtcg ggaccgggtt ctcgaccatc gactcgagca tctgcgtcgc gacgatcacc
     2581 gggaccgcct tctcgcgccc gaccgcgagg atcgccctct gaacgcccgg gaccgccgcg
     2641 aggccgatct ccacgccgag gtcgccgcgc gcgaccatca ggacgtccga cgccgccgcg
     2701 atctcgcgaa gtgcgcggac ggcctcgacg cgctcgatct tcgagacgat ccagggccgg
     2761 cgctcggcgg gagcggccgc gaggaggcgc cggagcgcct tcacgtgatc cacgctcctc
     2821 acgaacgaga ggccgacgaa ctggatgccg gcctcgagcc cctcgcggag gtcgcgccgg
     2881 tccttcggcg tgaccgccgg cgccgagagc gccgagctcg gcaggttcac gccggccccc
     2941 ttccggaccg tacctcccgc gagcacccgc gcccgcagcg ccccgcgctc ccgcctctcc
     3001 accttcagcc ggatcagacc gtcggcgatc cggatctccg cccccggcgc cacctcggag
     3061 agaagggccg ggtaggtcac ggagatcgtc cggccggagc ccatcgcgtg ccccgggacg
     3121 atctcgacgg cgtctccggc ctcccaggcg gtcgtgtcgc cttcgatcgg accgacgcgg
     3181 atcttcggcc cgccgaggtc gaggaggacg ccgaccggcc gcccgagggc cttctcggcc
     3241 gaccggacga gggcgacgcg ccgcgcgatg tccccgggcc ggcagtgcgc tccgttgagc
     3301 cggacgacgt cgaccccggc ggagaggagt cggacgagct cgcctcccgt ctcggcacgc
     3361 gggccgatcg tggcgacgat cttcgtggcg cgaggctccc ggatcggctt ggccatctct
     3421 tccctccggc ggaccttatg atctctcgat ggctgagatt ccggaatccc atacgggctc
     3481 ccatctcgtg ctccacgacg ccccggcgcc gaccgatccg cgcatcgacc agatcgccgg
     3541 ccacgtgagg gagatcatcc gcattctcgg cctctctccc gaggcggacc acaaccttcg
     3601 cgacaccgac cggcgcgtcg cgaagatgta cctcgagatc ttctccggcc tcgaggaggg
     3661 gaacttcccg gacctgacga ccttcccgaa cgaggagggc taccaggcca tggtcatcga
     3721 gaaggagatc cccttctact cgatgtgcgc ccaccacttc gtcccgttct acgggcacgg
     3781 ccacatcgcc tacatcccgc aggagcggat cgtcggcctc tcgaagctcc cgcggatcct
     3841 ggagttcttc gcccgcaggc cccagctcca ggagaggctg accgagcagg tcgcgggggt
     3901 cctctgggag gagctccggc cgctcggcgt catggtcgtc atcgaagccc gccacctctg
     3961 cgtcgagatg cgcggcgtga agaagccggg ctcggtcacc gtcacgtccg ccctccgcgg
     4021 agtcttcaag cagaagacgg tccgcgagga gtttctggat ctgctccgaa ggaggggatg
     4081 agcgtcgaag cgcccgcggc agcggggggg gccggagacg cgcttcagcg tagccagccg
     4141 gggaaggcgc cggcctttcg ggcttcctcc ccgagctctt cccaccgctg ctgctccccg
     4201 aggtgccgct ggcgggcgcg ttcggcccgc tcccgcgcct cgagaaggta gaggacgtac
     4261 gtcgtcgtct cggcctcgct caggccgagc caggcccgct ccgcctccgc gagcgtccgc
     4321 tcggcgatct gccggtcggc cgccgccgcc tcgagggcct cgcgccgctc gtccgcccgc
     4381 tcccgccagt agcgctcgcc acggccgaga aggtctgtcg gctcggcgac ctcgaccgga
     4441 gggacttcgc gcgcgggtcg aggcggagcg ggggtcccct tacgcgcccc ttcgatgtgc
     4501 gcgcgggcct cttcggcgga ggtccggagc ttcacgaggt ttccgagggg gggcgtctcg
     4561 aacggcccgg gggcgggcgt cgccgccgga ggaggggtcg gcttcggcgc tcccggctcg
     4621 ttcgccgcac gcgtcgccgc ctcgtcgatc cgcgcgagcg gaagggagac gagcgcgccg
     4681 cccgaaagga gccgtccgac gagcctcgtc cccctgcgca ccgggggcga gtcgaagacg
     4741 acgcgtgcgc cgtccgactg gacgaccacc cacgacccgg ccgccttctc tccgccggcg
     4801 gcgctcccgg cgagcacggc caggatgccg acgagcgcgg cggagagcgc accggccttc
     4861 acgggacgct tccgcgcagg agcgctccgg ccgcctcgag gccgcggagc gtcctcacgc
     4921 cgtgccagca gaaatcgtcg ccggagacga gctcgacacg cgattccggg agcagcccgc
     4981 ggaagagcgc ccgctccttc tccccgaatc ggtacggctc gtcggggagg acgacgagcc
     5041 cgggcgcgag agcggcgagc tcggcctcgt ccgtccgcgg ccacgggccc cgctcgcgcg
     5101 cgaagacgtc cacaccgccg aacgtctcga ggaggtcggc gatgtaggtc ccggcggcgg
     5161 cggccatcca cggccccttc cagatcaggt aggcgaagcg gaccgggcgc gccgccggac
     5221 gggccgccgc gagacgctcc tccacccgac gcgcccatcc ctccgccgcc ggctcgcttc
     5281 ccgtcagggc gccgaggcgg cggaggagcg gagggacgtc cgcgacgcgc gtcgggtggc
     5341 tcacgtcgac gcggtggcgc gcggccagcg cggcgacgtc ggcctcgcgg ttctcctccg
     5401 cgttgcacag gacgaggtcg ggcgccagcg cgccgatcgc ggcgtggtcg gggtccttcg
     5461 tcccgccgac ccgcgggatc ccctcgagcg cctcgcgggg gtggacgcag tagcgcgtca
     5521 cccccgcgag gctcccgccg aggccgaggg cgacgagaga ctcggtcgtc gacgggcaga
     5581 gggagacgat ccgcatcagg cggccggctt cttcctcccg aggagcccgt cgaggacctc
     5641 cttcgaggac ctcttcacgt cctcgtggag cgagtcgccg tgggcgtcca tcgtgacgat
     5701 gagcgggaag tccttcacct cgtagatcca catcgcctcg gggactccga gctcctcgag
     5761 gtgcttcacg ccgaggacgc ggacgacccg ccgcgcgagg acctgcgcgg ccccgccgtg
     5821 ggcgtggagg tagacggcgc cgtgctcctt caggccggcg agcgtcttca gccccatccc
     5881 ccccttgccg acgacgccgg cgaagccgta cttgccgatc acggcggcct gatagggctc
     5941 ctcgcggatc gaggtcgtcg gcccggcggc gaggacgctc caggagccgt ccttctccct
     6001 ccgcatgacc ggcccgcagt ggtaaaggat cgacccgcgc aggtcgaggt cggggtactc
     6061 gggccagctc ttcgtccaga gcttgtggag ggcgtcccgc cccgtcacca tgagccccga
     6121 gacctccacg gcgtctcccc gcttgaggga gcgcacgacc tccggggaga ggggcgtcgt
     6181 cagctttctc gcctcgctca ttgcgcgtac accgcctttc cggagggcga gacctcgacg
     6241 gaggcccgcc gcgtcgccca gcacatgtag ctgacggaga cgaaatacga cgccggaagg
     6301 cgcgcgaggt gggacatctt cacgccgagg agcgtcgtct tcccgccgta ccccatcggg
     6361 ccgacgccga ggaggttccc cttctccagg agctcctcct ccgccgccgc cagggccggc
     6421 tccgcgtgac ggtctcccag cttgcggaag agctggcgct tcgcctcggc catgccgctc
     6481 gcgcggtcgc cgcccatgca gacgccgagg atgccggggg cgcacccctt cccctgcgcc
     6541 tgaaagaccg cgtcgaggac gcagcgcttc accccctcga ggtcgcggtc ggccccgagc
     6601 tccgtgctcg ggagcgagta ctgcgccccg acgttctcgc agccgccccc cttgaggatg
     6661 aggtcggcct tcagcccggc gcgcgcgtgc tcgtggacgt ggacgatcgg catcccgcgt
     6721 cccggctcgt tcccggtgtt cttccccgac agcgtgtcga cggagttcgg acggagatac
     6781 cccttcgccg tcgcgatccc gaccgccttc gccgcggccc gctcgatcgc gccgatcgag
     6841 acgccgcgcg gggcggcgac ctcgaagagc ggcattcccg tgtcctgaca gaccgggcgc
     6901 gacgtctcgt cggcgagccg cgagttctcg aggaggaccc cgagcgtctc gcgggccacg
     6961 gaaccctcgt cttcccggtc gcgcgccgcc tcgagggcgg cgaggacgtc gggtggcatg
     7021 cacgtcgcgg cgcgacggac gagctccgtg aacgtcgcga ccagcgcttt ctcgccgaac
     7081 gggtcggccg cgggggcctt cttcgtcgcc ggcgccgggc gggtcgcctt ccggacgggc
     7141 gccctcacgg ccctcttcgc cggcggagcc gcggtccgtt tcgcgggggc gggcttgcgg
     7201 gcggcggcgc gcttcttcgc cggcgccggc cgacccggct tct
//
LOCUS       DAOTEZ010000208         7226 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_7362_length_7226_cov_6.075722, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000208 DAOTEZ010000000
VERSION     DAOTEZ010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7362_length_7226_cov_6.075722"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..964)
                     /gene="truB"
                     /locus_tag="P5164_11670"
     CDS             complement(35..964)
                     /gene="truB"
                     /locus_tag="P5164_11670"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRY44591.1"
                     /translation="MSVPSADGPSGLLLVDKPEAMTSHDVVDAVRRLLGTRRVGHTGT
                     LDPAATGLLVLCVGRAGRLQSFLTCYDKSYEGTMRFGVETDTYDREGTPVGTPHPDPR
                     PDEERLRAACSRWVGEFEQAPPPFSAKKIGGKKFYELARRGEAVPHLPKKIRITRFDA
                     VEVSGDVARFHVDCSSGTYIRSLAHDVGKELGLGAHLASLRRTRIGPFSLDRARTLPA
                     LEALAPAERTAPPWWMPLAEVPLPFPSLALLPGEAAKVRRGHAVPVRVPTDATGAPWV
                     RLLAEGDLVALGHLEPLGRGALALAKPKIVLQD"
     gene            complement(961..1941)
                     /locus_tag="P5164_11675"
     CDS             complement(961..1941)
                     /locus_tag="P5164_11675"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HRY44592.1"
                     /translation="MTDFEAVTALLSSGRRFLLTGHRNPDGDSLGSALALAQALVARG
                     KQAHVVMRDRWSSSYDGMPGVADVAVSEALPPDWPAAWDAAIVMECPEAERPGFPELL
                     SGTTVNVDHHPGNTRWATHNLVIEPAAAVGEVVADLLDRLEWPMTPAIATNLWVSLVS
                     DTGSFRYGNTTPRALALGARLVTEGVRPDVVNEFLFEAKPLSTVKLESLVLGTLELHD
                     EGRVALVSLPKRFLAESGADAADGEGLVNRARGIDGVKAAALVRESDETGVFRCSLRS
                     KGSVDVRSVAAERSGGGHRNAAGCRVNGTWESVKADISRALAAAVAAEPR"
     gene            complement(1944..2330)
                     /gene="rbfA"
                     /locus_tag="P5164_11680"
     CDS             complement(1944..2330)
                     /gene="rbfA"
                     /locus_tag="P5164_11680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRY44593.1"
                     /translation="MKERRRPRQVADVVREELARLLRTEVSDPAVGFATITDVVLSPD
                     LRSARVFVSVLGGEGAFEKSVAALNRAAPMLRGMVGRNCRLRHSPELRFEEDHSLERG
                     ARIEELLRHLPHPEGEGGDDDGEKKG"
     gene            complement(2327..2674)
                     /locus_tag="P5164_11685"
     CDS             complement(2327..2674)
                     /locus_tag="P5164_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010120224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HRY44594.1"
                     /translation="MVVALAVFDFHLPTCRGLKEKRAFLRPLKARLRGGFEISAAEVA
                     HHDLLQRAAVGVAAVGPDRSGLEPLLGKVVEFVEAWAEESGVELTSLRSEFLEYGDVG
                     AVGSSFGAEQEAS"
     gene            complement(2745..4850)
                     /gene="infB"
                     /locus_tag="P5164_11690"
     CDS             complement(2745..4850)
                     /gene="infB"
                     /locus_tag="P5164_11690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRY44595.1"
                     /translation="MPPPVPGTPDRRRKDAVKGGSAVPPKKDDKKTASKKLRTKEASA
                     LATNVRDFLGTYQEDTYEDVPGAIPGEGEEVDPNRPISKSAQRRAGKRTMMTETGDVV
                     EVKKDQSGPVFLSEGVTVKELSEKTGILAKDLVKAFLGRGILAAINHPVNPTLAVEVA
                     RDFGIEAAVVSFEEDLELSRQQAQTEADAESAASTADKKPRAPVVTVMGHVDHGKTSL
                     LDSIRKAKVAEGEAGGITQHIGAYRVQAKGRAIVFLDTPGHEAFTMMRARGARATDIV
                     VLVVAADDGVMPQTVEAIDHARAAKVPVVVAINKIDKPEANVMRVKQALADKGVLVEE
                     YGGEIVACEISAKKQIGIDALLEMILLQADLLELKAAVDGPARGVVLEARREVGRGTL
                     ATVLVQQGTLKVGDIFFAGSAVGRVRAMLDDRGQRVLEAGPATPVEVMGFDDIPNSGD
                     SLQVVEDESKARQVTGFRQQKEREEALAKSSRLSLDSLFSRMAKGEVKELPIILKADV
                     HGSEEVITQSLNKLSTAKVKVNVLHSGVGAISVNDVLLASASEAIVIGFNVRPERKAQ
                     ELAEKEGVDIRLYSVIYNLTDEIKKAMAGLLDSVQKEVAKGRAEVRETFRIPKIGLIC
                     GCMVVEGVIPRGANARLLRDNRVIYEGKIGSLRRFKDDASEVKSGFECGIGIAGYQDV
                     KVGDVIEAFVTEEVAASLT"
     gene            5002..>5580
                     /locus_tag="P5164_11695"
     CDS             5002..>5580
                     /locus_tag="P5164_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44596.1"
                     /translation="MGRATARIVAALGGAMTARPETPDRAAGAAGVWGAGRCATGGAE
                     EAADESGAETGWLEGAVALGAVGSDARLRIRPSAPDGAGGRAPGVAGFWAAARRLAAD
                     AVWIPPVGRRMAPLADPAAPAPGAVAPAAAAAADAEGAVVAAGAGGAVRVVAADRLGA
                     CLAGCAAGAVGGAETGSGGTARGASGAGAGSAA"
     assembly_gap    5581..5590
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5591..6174)
                     /locus_tag="P5164_11700"
     CDS             complement(<5591..6174)
                     /locus_tag="P5164_11700"
                     /inference="COORDINATES: protein motif:HMM:NF016635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HRY44597.1"
                     /translation="MSAKFTVTELARMMGKPAKEVLFLLQGIGVDVKSADSVLDPGTA
                     QAILTGKTQAPKTLIVRQTPVAPPPSAGAAGTPKPKAERAALKRIKIVEKAPDTESAP
                     EAEAVAAPAEPVAVPAVVAEQSPTVPEPAAPEPPAPVEAPPTPRKPAPAKTAPLARPE
                     PAAEPPAAKATAEAAVVEKAAEPKPEPPQAEVTPP"
     gene            complement(6180..>7226)
                     /locus_tag="P5164_11705"
     CDS             complement(6180..>7226)
                     /locus_tag="P5164_11705"
                     /inference="COORDINATES: protein motif:HMM:NF024582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HRY44598.1"
                     /translation="RTEAVIPREHQSRAERYTQGERVRGVLVDVHRNPKGAQVVLSRT
                     APELLMKLMEMEVPEIYDGTVVIRGCVRQPGDRAKVAVSSRERDVDPVGACVGMRGAR
                     VQAITRELRGERIDIIQFSEDIIAYAQNALSPAKITRVSLMAAEEETVEEGEETLPTL
                     ECIVEEDQLSLAIGKRGQNVRLASALVGARIDIKSEQAVKEQVAQALQRMLLASQRRG
                     TALSDVAGLEPAALEALAAGGFETVGEVLDADTGEDEEAMGRLDAAIADEDSREEALE
                     ALRAFESAPAEETEEDEDDFLASDDEESEEETGEDDEGSSEPDVEDGPADESEETASE
                     EPGNAGPDTENPEK"
BASE COUNT          899 a         2453 c         2787 g         1077 t
ORIGIN      
        1 cgggccgaca ggccccggtt cccccgggaa catttcagtc ttgtagcacg atcttcggct
       61 tcgcgagggc gagcgcgccg cggccgagcg gctcgaggtg accgagggcg acgaggtcgc
      121 cctcggcgag gagccggacc cagggtgcac cggtcgcgtc cgtcgggacg cgaaccggca
      181 cggcgtgccc tcgccgcacc tttgccgcct cgccgggaag gagggcgagg gacggaaagg
      241 ggagcgggac ctccgcgaga ggcatccacc aggggggcgc ggtcctctcg gcgggggcga
      301 gcgcctcgag cgcggggagc gtccgggccc gatcgagcga gaacgggccg atgcgcgtcc
      361 ggcgaaggga cgcgaggtgc gctccgaggc cgagctcctt cccgacgtcg tgagcgagag
      421 accggatgta ggtgccggag gagcagtcga cgtggaatcg cgccacgtcg cccgagacct
      481 cgacggcgtc gaagcgcgtg atccggatct tcttcggcag atggggaacc gcctcgccgc
      541 gacgggcgag ctcgtagaac ttcttcccgc cgatcttctt cgccgagaac ggcggagggg
      601 cctgctcgaa ctcgccgacc cagcgggagc aggcggcccg cagccgctcc tcgtcgggcc
      661 gggggtcggg gtgtggcgtt ccgacggggg tcccttcccg gtcgtaggtg tcggtctcca
      721 cgccgaacct catcgtccct tcgtagctct tgtcgtagca ggtgaggaac gactggaggc
      781 ggccggctcg gccgacgcac agaacgagaa gccccgtcgc ggcggggtcg agggtcccgg
      841 tgtgcccgac gcgccgcgtt ccgaggaggc ggcggacggc gtccacgacg tcgtgcgagg
      901 tcatcgcttc cggcttgtcg acgaggagga ggccggacgg cccgtcggcc gaaggaacgc
      961 tcatcgcggc tccgcggcga ccgcggcggc gagcgcgcgg gagatgtcgg cctttacgga
     1021 ctcccatgtc ccgttcacgc ggcagccagc ggcgttccgg tggccgcctc cgctccgctc
     1081 ggcggcgacg gagcgaacgt ccacggagcc cttggagcgg agcgagcagc ggaagacgcc
     1141 cgtctcgtcg ctctccctca cgagggccgc ggccttcacg ccgtcgatcc cgcgggcccg
     1201 gttcacgagc ccttcgccgt ccgccgcgtc ggcgcccgac tcggcgagga agcgcttcgg
     1261 cagggagacg agcgcgacgc gcccctcgtc gtggagctcg agcgtgccga ggacgagcga
     1321 ctcgagcttc acggtcgaga gcggcttcgc ctcgaagagg aactcgttga cgacgtcggg
     1381 ccggacgccc tccgtcacga gccgggcgcc gagcgcgagg gcgcggggcg tcgtgttgcc
     1441 gtagcggaag gagcccgtgt cggagacgag ggagacccag aggttcgtgg cgatcgcggg
     1501 cgtcatcggc cactcgaggc ggtcgaggag gtcggcgacg acctcgccga cggcggcggc
     1561 cggctcgatg acgaggttgt gggtggccca tctcgtgttg cccgggtggt ggtcgacgtt
     1621 gacggtcgtc ccggagagga gctccggaaa gcccggccgc tcggcctcgg ggcattccat
     1681 gacgatcgcg gcgtcccagg cggccggcca gtcgggcgga agggcctcgg agaccgcgac
     1741 gtccgcgaca cctggcatcc cgtcgtagga gctcgaccag cggtcgcgca tcacgacgtg
     1801 cgcttgcttc ccgcgcgcga cgagcgcctg agcgagagcc agcgccgagc cgagcgaatc
     1861 gccgtccggg ttccggtggc ccgtcaggag gaaccggcgg cccgaggaga ggagcgcggt
     1921 gacggcctcg aagtccgtca cgctcacccc ttcttctctc cgtcgtcgtc tccgccctcg
     1981 ccctcgggat gcggcaggtg ccgaaggagc tcctcgatgc gcgcgccgcg ctcgaggctg
     2041 tggtcctcct cgaagcggag ctcgggggag tggcgcagtc ggcagttgcg tccgaccatg
     2101 ccgcgaagca tcggggcagc gcggttcagc gcggcgacgc tcttctcgaa cgccccttcg
     2161 cccccgagga ccgagacgaa gacccgggcg gagcggaggt cgggggagag gacgacgtcg
     2221 gtgatcgtcg cgaagccgac ggcggggtcg gagacttcgg tcctgaggag ccgggcgagc
     2281 tcctctcgga cgacgtcggc gacctggcgg ggacggcggc gttccttcac gaggcctcct
     2341 gttcggctcc gaacgaggag ccgacggcgc cgacgtcccc gtattcgaga aactcgctgc
     2401 gcagcgaggt gagctcgact ccggactcct cggcccacgc ctcgacgaac tcgacgacct
     2461 tcccgaggag cggctcgagc ccggagcggt ccgggccgac cgcggcgacg ccgacggcgg
     2521 cgcgctggag gaggtcgtgg tgggcgacct cggcggccga gatctcgaag ccgccgcgca
     2581 ggcgcgcctt gagcggccgg aggaaggccc gcttctcctt gagcccgcgg caggtcggga
     2641 ggtggaaatc gaagacggcg agggcgacga ccatcggttc tccgggacga cgaggctccg
     2701 gggagtcctc cccggggcgc gagccccggg gaggacttcc ggccctacgt cagcgaggcg
     2761 gcgacctcct cggtcacgaa ggcctcgatg acgtcgccga ccttgacgtc ctggtagccg
     2821 gcgatgccga tgccgcactc gaagccgctc ttgacctcgg aggcgtcgtc cttgaagcgg
     2881 cgcagggagc cgatcttccc ctcgtagatc acgcggttgt cgcggaggag ccgggcgttc
     2941 gcgccgcgcg ggatgacccc ttcgacgacc atgcacccgc agatgaggcc gatcttcggg
     3001 atgcggaaag tctcgcgcac ctcggcgcgg cccttcgcga cctccttctg gacgctgtcc
     3061 aggaggccgg ccatcgcctt cttgatctcg tcggtgaggt tgtagatgac cgagtagagg
     3121 cggatgtcga cgccttcctt ctcggcgagc tcctgggcct tgcgctccgg cctcacgttg
     3181 aagccgatga cgatggcctc ggaggccgag gcgagcagga cgtcgttcac ggagatcgcg
     3241 ccgacgccgg agtggaggac gttcaccttc accttggccg tcgagagctt gttgagcgac
     3301 tgggtgatga cctcctcgga gccgtggacg tcggccttga ggatgatcgg gagctccttg
     3361 acctcgccct tggccatccg gctgaagagc gagtcgagcg agaggcgcga ggacttggcc
     3421 agggcctcct cccgctcctt ctgctggcgg aagccggtga cctgccgggc cttcgactcg
     3481 tcctcgacga cctggaggga gtcgcccgag ttcgggatgt cgtcgaagcc catgacctcg
     3541 acgggggtcg ccgggccggc ctcgaggacg cgctggccgc ggtcgtcgag catcgcgcgg
     3601 acgcggccga cggccgatcc ggcgaagaag atgtcgccga ccttgagggt gccctgctgg
     3661 acgaggacgg tggcgagggt gccgcggccc acctcgcgcc gcgcctcgag gacgacgccg
     3721 cgggcgggcc cgtcgacggc ggccttcagc tcgaggaggt cggcctgcag gaggatcatc
     3781 tcgaggaggg cgtcgatgcc gatctgcttc ttcgcggaga tctcgcaggc gacgatctcg
     3841 ccgccgtact cctcgacgag gacgcccttg tcggcgaggg cctgcttgac cctcatgacg
     3901 ttcgcctcgg gcttgtcgat cttgttgatg gcgacgacga ccgggacctt cgccgcgcgg
     3961 gcgtggtcga tcgcctcgac ggtctgcggc atgacgccgt cgtcggccgc gacgacgagg
     4021 acgacgatgt cggtcgcccg cgcgccgcgg gcgcgcatca tcgtgaaggc ctcgtggccc
     4081 ggcgtgtcga ggaagacgat cgcgcggccc ttggcctgga cgcggtaggc gccgatgtgc
     4141 tgcgtgatgc cgcccgcctc gccctcggcg actttcgcct tgcggatgga gtcgaggagg
     4201 gacgtcttgc cgtggtcgac gtggcccatg accgtgacga ccggggcgcg gggcttcttg
     4261 tcggccgtgg aggcggcgct ctccgcgtcg gcttccgtct gggcctgctg gcgggagagc
     4321 tcgaggtcct cctcgaacga gacgaccgcg gcctcgatgc cgaagtcgcg ggcgacctcg
     4381 acggcgagag tcgggttcac ggggtggttg atcgcggcga ggatcccgcg tccgaggaag
     4441 gccttgacga ggtccttcgc gaggatgccg gtcttctcgg agagctcctt gacggtgacg
     4501 ccctcggaga ggaagaccgg gccgctctgg tccttcttga cctccacgac gtcgccggtc
     4561 tcggtcatca tcgtccgctt cccggcgcgg cgctgggccg acttggagat cgggcggttc
     4621 ggatcgactt cctccccttc gccggggatg gcgcccggga cgtcctcgta cgtgtcctcc
     4681 tggtaggtgc cgaggaagtc acggacgttc gtggcgaggg cggaagcctc cttcgtccga
     4741 agcttcttgg aagcggtctt cttgtcgtcc ttcttgggcg ggacggcgct gccgcccttg
     4801 accgcgtcct tgcggcgacg gtccggggtg ccggggacgg gcggcggcat ggccggagcc
     4861 atcggtccgc gaccgagtcc accgggccgg ggagcgaagc cggggcgcgg ggcgaagccg
     4921 gggcgcgggg ccatcccggg ccgcatgtga ggaccgggag ccgcgacggg cgtcgcgggg
     4981 cgcgcgacgg gcgctccagg ggtggggcgg gccaccgcgc ggatcgtcgc ggcgctcggc
     5041 ggcgcgatga cggcgcggcc ggagacgccg gaccgggcgg ccggggcggc gggggtctgg
     5101 ggagcgggcc gctgcgcgac cggcggggcg gaggaggccg cggacgagag cggagcggag
     5161 acaggctggc tcgaaggggc cgtcgccctc ggcgcggtcg gctcggacgc tcgcttgagg
     5221 attcgcccca gtgcgccgga cggggccgga ggccgggctc cgggcgtcgc cggcttctgg
     5281 gcggcggcac gcaggctcgc ggcagacgcc gtctggattc cgccggtggg gcgccggatg
     5341 gcgccgctgg cggatcccgc ggctcccgca cccggagcgg tcgcgccggc cgctgctgcg
     5401 gcggcggacg cggaaggggc cgtggtcgcc gcaggcgcgg gcggtgccgt gcgagtcgtg
     5461 gccgcggacc ggctcggggc gtgcctcgcc gggtgcgcgg ccggcgccgt ggggggcgct
     5521 gagacgggtt ccggcgggac ggcccggggt gcctccgggg ccggcgccgg ctcggccgcg
     5581 nnnnnnnnnn ggaggtgtca cctcggcctg agggggctcc ggcttcggct ccgcggcctt
     5641 ctcgacgacg gcggcctcgg ccgtcgcctt ggcggcgggc ggctcggcag cgggctccgg
     5701 ccgggcgaga ggcgcggtct tcgccggggc cggcttccgg ggggtgggcg gcgcctcgac
     5761 cggagcgggg ggctccggcg cggccggctc gggaacggtc ggagactgct ccgccacgac
     5821 cgccgggacc gcgacgggct ccgcgggtgc tgccacggcc tccgcttcgg gcgccgactc
     5881 ggtgtccggc gccttctcga cgatcttgat ccgcttcagg gcggcgcgtt cggccttcgg
     5941 cttcggcgtc cccgcggctc ccgcggaggg agggggggcg accggcgtct gccggacgat
     6001 gagcgtcttg ggagcctggg tcttgcccgt caggatcgcc tgggcggtcc cggggtcgag
     6061 caccgagtcg gcggacttga cgtcgacgcc gatcccctgc agaaggaaga ggacctcctt
     6121 cgcgggcttt cccatcatcc gggcgagctc ggttacggtg aacttcgcgg acatcgtctt
     6181 cacttctcgg ggttctccgt atccggcccg gcgtttccgg gctcctcgct cgccgtctct
     6241 tccgactcgt ccgcgggacc gtcctcgacg tcgggctcgg aggagccctc gtcgtcttcg
     6301 cccgtctctt cttccgattc ctcgtcgtcg gaggcgagga agtcgtcctc gtcctcctcg
     6361 gtctcctcgg ccggagccga ttcgaaggcg cgaagggcct cgagggcctc ctcgcgcgaa
     6421 tcttcgtccg cgatcgcggc gtcgaggcgg cccatggcct cctcgtcctc gccggtgtcg
     6481 gcgtcgagga cctcgcccac cgtctcgaat ccaccggccg cgagcgcctc gagcgccgcc
     6541 ggctcaaggc ccgcgacgtc cgagagcgcg gtcccgcgcc gctgggaggc gaggagcatc
     6601 cgctggaggg cctgggcgac ctgctccttc acggcctgct cgctcttgat gtcgatccgg
     6661 gcgccgacga gcgcggaggc gagccggacg ttctgcccgc gcttgccgat ggcgagcgag
     6721 agctggtcct cctcgacgat gcactcgagg gtcggaagcg tctcctcgcc ctcctcgacc
     6781 gtctcctctt ccgcggccat cagcgagacg cgcgtgatct tggcggggga gagggcgttc
     6841 tgggcgtacg cgatgatgtc ttccgagaac tggatgatgt cgatccgctc cccgcgcagc
     6901 tcgcgggtga tcgcctggac gcgcgcgccg cgcatgccga cgcaggcgcc gaccgggtcg
     6961 acatccctct cgcgggagga gacggcgacc ttggcccggt cacccggctg gcggacgcag
     7021 ccccggatca cgacggtccc gtcgtagatc tcggggacct ccatctccat gagcttcatg
     7081 aggagctcgg gagcggtccg cgaaaggacg acctgggcgc ccttcgggtt ccggtggacg
     7141 tcgacgagga cgccccggac tcgctcgccc tgcgtgtagc gctcggcgcg ggactggtgc
     7201 tccctcggga tcacggcctc ggtgcg
//
LOCUS       DAOTEZ010000209         7190 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_7411_length_7190_cov_5.345340, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000209 DAOTEZ010000000
VERSION     DAOTEZ010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7190
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7411_length_7190_cov_5.345340"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="P5164_11710"
     CDS             complement(<1..287)
                     /locus_tag="P5164_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase
                     catalytic subunit"
                     /protein_id="HRY44599.1"
                     /translation="MPTETLRTVCNRDCPDACGLIATVEDGRLVRLGGDPAHPITRGF
                     LCSRTSQFPARQNSPDRLTVPLLRDGKGFRPVSFEEAIAFAAERLLAIRAES"
     gene            400..1257
                     /locus_tag="P5164_11715"
     CDS             400..1257
                     /locus_tag="P5164_11715"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44600.1"
                     /translation="MAEPFAVIEAHGRHAQRALFTAGGETLVTCGQDAAIRLWKVPSF
                     APDGAFEGHAASVNTLSFSADGGLLASGSSDGTARIWSFPGGRELDVLDGQVAAAASP
                     AGGFATISTKARALLWDGNYDLVATLPLLDRRLFSLAFTPDGSALLVAGTGTVHRVDV
                     KGRTKAGEYPGHQTAVASMSLSPDGTLLATTGAEGTLRLRRLDGGTERFAVRVGRPGV
                     LQTAFSPDGAVIAVSIDFAIQLRSAADGALLSTIEVPMKGVYGLGFSPDGRFLANAAA
                     DGRIRVFAL"
     gene            1415..3382
                     /locus_tag="P5164_11720"
     CDS             1415..3382
                     /locus_tag="P5164_11720"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRY44601.1"
                     /translation="MRSAPEGASGRRPRTAREARRLVGEAAREIAAAPAAWAAVVNRA
                     RAEFGRPPLGPEAVSALGFPLLSAVDDGRPALLPLERLEPLGKGGMNTVSRSADLLTG
                     RPLVVRRLIPAESAVEEEMRRRRNELVRERLSVLRRHPVLQRFWGCGLVEGEACEIFD
                     FAPGTTLAQHVRAKGLTYGALLDVGWHAARGLLHLSRHGLVHGDVKPENFCVEERGSG
                     GSARLRVSLIDFDIVSTVDEQLFQYRLSNTLDGTLPYMPPENFVQAVPEDPDECRRMV
                     LAKDVFALGMSLVRVVNGRFPDGVYTSMTSLVQKKERLEEMPVSLPASLPADLRELVL
                     AMCRTEWRMRPPLPVVVRTFDVLRRGATEEERARLVAAPRVEALPEAPPSEPVELVGP
                     YRLVNRAFAERTPGDGQTRPLSELHDPFGRRLVGVPYEFRTAEEESAFYEERKALLED
                     LNAVRLRHPELFPGSFRDLVRQGIGEDLFVVWVVRPLLEGAIDLDRYLREERPDASLG
                     ERIAILRRVVEALSALESGGYTLAALTPRLVFFVPPPASGGDAAPTIAGRTRPVAALF
                     DIGGPASGRRFRQELMGTASAVRSSPEATGRVVAGFFDVASRMRVLTELDRDGRDAFL
                     QLGDAQSWAERADLLRWLETRLGERFPPAGP"
     gene            complement(3630..3893)
                     /locus_tag="P5164_11725"
     CDS             complement(3630..3893)
                     /locus_tag="P5164_11725"
                     /inference="COORDINATES: protein motif:HMM:NF025764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Franean1_4349 family RiPP"
                     /protein_id="HRY44602.1"
                     /translation="MSQGNVERIVGRLVTDEDFRREFRADPERVVRSLAERGFELTRA
                     EVAALVTLDALTFDRFADALDPRLQKASLRAPVPAPRAAGRPA"
     gene            4128..4757
                     /locus_tag="P5164_11730"
     CDS             4128..4757
                     /locus_tag="P5164_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44603.1"
                     /translation="MKATRILVVAGACALVFSTACGKKETPESEGKELSKNPIAAMGQ
                     IQEAVEKAAQAAKESQEMKAVDPVHFSKLIELLPKAPAGFTADGEPRGETTNAMGFKV
                     STAEQSYSAEGKSLQVKLTDGAFNAPLYTFVTMASQFARESTEGYEKGVTYDGNPGVE
                     KWRKDGGDAELNMVVGKRFFVEVQGSGITPELVREVWSSIDRTTLAGLK"
     gene            4765..5862
                     /locus_tag="P5164_11735"
     CDS             4765..5862
                     /locus_tag="P5164_11735"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRY44604.1"
                     /translation="MTNIGIPKECRPGEFRVGISPVGVRMLTGAGHTCFVQSGAGDGS
                     GFLDEQYREAGATVVHSAEEAWRRADLVLKVSRPTVQELEWLQPGKTLMGYLYLAAAH
                     PGKVELLLEKKVSAVAYELIQLADGSLPVLKPLSQIGGRMAAQVAARLLQNDAGGHGR
                     LIGGVAGVPPTEVVVVGAGVAGEAAARAFLGLGAHVTVLDTDLARLQSIDERLSGKLV
                     TVVAHPFHVSRAAEYADVLVGAVMVPGERAPIVFTRAMLRKMKPGSLFLDLSIDQGGC
                     AETSRPTTHESPTYVEDGVVHCCIPNLGGVVARTSTHAFLNAAAPWIERVVVQGIEAA
                     AAADPALAHGLVTHAGELRNFRRVSYARPVE"
     gene            5885..7189
                     /locus_tag="P5164_11740"
     CDS             5885..7189
                     /locus_tag="P5164_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRY44605.1"
                     /translation="MSWLHTYHDRIVSAEEAVRTVKSGDRVYLTGNCSVPQALMKALI
                     QRAPELTDVEITHILTLGKTPYADPEFAGHIRVNTMFIGEGVRGAVNEGRADFTPVRL
                     SEVPKLFSEGIVPLDVAFIHVTPPDEHGFCSYGVEVSTNRAAASAAKLVVAEINPQMP
                     RTLGDAFIHISRIHRIVPVDYPIIETKMGTPSELSMRIGKFVADLIEDGSTMQMGIGA
                     IPDGVLYYLKDKKDLGIHTEMFSDGVVELYEKGVVTGEKKNIDRGKIVAGFLIGSKRL
                     YDFVDNNPVVEMRPTEYVNDPYVIRQNDRMVAINSAIEVDLTGQVCADSIGTRLYSGV
                     GGQLDFIRGAARSRGGKPIIGLPSSGTGKDKQAFTRIVPMLKHGAGVVTGRYDVHYVV
                     TEWGVANLYGRTIRQRAKALIDVAHPDFRAELERKAIELKYI"
BASE COUNT         1053 a         2493 c         2627 g         1017 t
ORIGIN      
        1 gactcggcgc ggatcgcgag gaggcgctcg gccgcgaagg cgatcgcctc ctcgaaggag
       61 acgggccgga agcccttccc gtccctgagg agcgggacgg tcaggcggtc gggcgaattc
      121 tgccttgcgg ggaactgcga cgtccgcgag cagaggaagc cgcgggtgat cgggtgcgcc
      181 gggtcgccgc cgaggcggac gagccgtccg tcctcgacgg tcgcgatcag gccgcaggcg
      241 tcggggcagt cgcgattgca gacggtgcgc agcgtctcgg tcggcatggc gcggatggta
      301 atcggccggt gccgcgggag cgaaacgggc cggctcgcgc ggctccgccc cgcgtatgct
      361 tcggcgtacg acgaccggag cggacgcggg aggacgaaga tggccgagcc cttcgcggtg
      421 atcgaggccc acggcaggca cgcgcagaga gcgctcttca cggccggcgg cgagacgctc
      481 gtaacgtgcg gccaggacgc ggcgatccgt ctctggaagg tcccctcctt cgctccggac
      541 ggagcgttcg agggacatgc ggcgagcgtc aatacgctct ccttctcggc agacggaggg
      601 ctcctcgcga gcggctcgtc ggacgggacg gcccggatct ggtcctttcc cggtggtcgc
      661 gagctggacg tgctggacgg ccaggtcgcc gcggccgcct cgcccgcggg cggcttcgcg
      721 acgatctcca cgaaggcgcg cgcgctcctc tgggacggga actacgacct cgtggccacg
      781 cttcccctcc tcgaccggcg gctcttctcg ctcgccttca cgcccgacgg gagcgcgctc
      841 ctcgtggccg ggacagggac ggtccaccgc gtcgacgtga aggggcggac gaaggccggg
      901 gagtacccgg ggcaccagac ggcggtcgcg tcgatgagcc tctcgcccga cgggacgctc
      961 ctcgcgacga cgggggccga ggggacgctt cggctccggc ggctggacgg cggcacggag
     1021 cgcttcgccg tccgcgtcgg acggcccggc gtcctccaga cggccttctc gccggacggg
     1081 gcggtcatcg cggtgtcgat cgacttcgcg atccagctcc gctcggcggc cgacggcgcg
     1141 ctgctctcca cgatcgaggt gccgatgaag ggggtctacg gcctcggctt ctcgcccgac
     1201 ggacgcttcc tcgcgaacgc ggcggcggac gggcggatcc gtgtcttcgc gctctgagcc
     1261 tttcgcgccc ggcggcgggc gtccgccgca ccgttctcgg gagagccccg accggctaga
     1321 ctgaggcgag cccggaggtg ccgaggatgg gtcgaccggc gcgtcgacgc gatcgagccg
     1381 ttcccgcgca gaggcgggag gagcaggggc gcgcgtgagg tccgctccgg agggcgcgag
     1441 cgggcggagg ccgcggacgg cgcgcgaggc gcggcgcctc gtcggcgagg cggctcggga
     1501 gatcgccgcg gcgcccgccg catgggccgc cgtcgtcaac cgggcacgcg ccgagttcgg
     1561 ccggccgccg ctcggcccgg aggccgtctc ggccctcggg ttcccgctcc tctctgccgt
     1621 ggacgacggg cgtccggcgc tccttccgct cgagcggctc gaaccgctcg gcaagggagg
     1681 gatgaacacc gtctcgcgct cggccgacct cctcaccggc cggccgctcg tcgtgaggcg
     1741 gctgatcccc gcggagtccg cggtcgagga ggagatgcgg cgccggcgga acgagctcgt
     1801 gagggagagg ctctcggtcc tccgccggca ccccgtcctg cagcgattct gggggtgcgg
     1861 cctcgtggag ggggaggcct gcgagatctt cgacttcgcg ccgggcacga cgctggctca
     1921 gcatgtgagg gcgaaggggc tgacgtacgg tgcgctcctc gacgtcggct ggcacgccgc
     1981 ccgcggtctc ctccacctct cgcgtcacgg gctcgtccac ggcgacgtca agccggagaa
     2041 cttctgcgtg gaggagcggg ggagcggcgg ctcggcgcgc cttcgggtaa gcctgatcga
     2101 cttcgacatc gtctcgaccg tcgacgagca gctcttccag taccgcctct cgaacaccct
     2161 cgacgggact cttccgtaca tgccgcccga gaacttcgtc caggccgtcc ccgaggaccc
     2221 ggacgagtgc cggcgaatgg tcctcgccaa ggacgtcttc gcgctcggga tgtccctcgt
     2281 gagggtcgtg aacggacgct tcccggacgg cgtctacacg agcatgacct cgctcgtgca
     2341 gaagaaggag cggctggagg agatgccggt ttcgctgccg gcctcgcttc cggcggacct
     2401 ccgcgagctc gtcctcgcga tgtgccggac cgagtggcgg atgcgccctc cgcttcccgt
     2461 cgtcgtccgg acgttcgacg ttctccgtcg cggcgcgacg gaggaggagc gggcccgtct
     2521 ggtcgccgcg ccgcgcgtgg aggcgctgcc cgaggcgccg ccgtccgagc cggtcgagct
     2581 cgtcgggccg taccggctcg tcaaccgcgc gttcgccgag cggactccgg gcgacgggca
     2641 gacgcggccg ctctccgagc tgcacgaccc gttcgggcgg cgcctcgtcg gcgtccccta
     2701 cgagttccgg acggccgagg aggaatccgc cttctacgag gagcggaagg cgctcctcga
     2761 ggacctcaac gccgtccgcc tccggcaccc ggagctcttc ccggggagct tccgcgacct
     2821 cgtgaggcag gggatcgggg aggacctgtt cgtcgtctgg gtcgtccggc ctctcctcga
     2881 aggggcgatc gacctcgacc gctacctcag ggaggagcgc ccggacgcct cgctgggcga
     2941 gcggatcgcg atcctgcggc gggtcgtgga ggcgctctcg gccctcgagt cgggcggcta
     3001 cacgctcgcg gcgctgacgc cccggctcgt cttcttcgtg ccgcctcccg cgtcgggcgg
     3061 tgacgcggcg ccgacgatcg ccggccggac ccgcccggtc gctgcgctct tcgacatcgg
     3121 ggggccggcg tcggggagac gcttccgcca ggagctgatg gggaccgcct ctgcggtccg
     3181 gtcgagcccg gaggcgaccg gccgcgtcgt ggccggcttc ttcgacgtgg cttcccggat
     3241 gcgggtcctg accgagctcg accgcgacgg gcgggatgcc ttccttcagc tcggcgacgc
     3301 gcagagctgg gccgagcggg ccgaccttct ccgctggctc gagacgcggc tcggcgagcg
     3361 attccctccg gcggggccct agaccggggc cgcttcgggg cgcttcgaga cgacgcgcgg
     3421 acggcgccga aaagagagac ggcggggtct gaccccgccg tcgaaggtcg ttgccccggc
     3481 gtcccggtcc tatcgcagga gagaagagga aggattgcga aaggtgtgga ccaggatgga
     3541 aggcggaaag gtgcggaagt ggtggaggtt ggacgggatg ggactcctcc gccaggtccg
     3601 ggagccgggg tagcgagcgt tctgggccgt catgcgggcc tcccggccgc tctcggagcg
     3661 gggacggggg cccggaggct cgccttctgg agcctcgggt cgagggcgtc tgcgaagcgg
     3721 tcgaaggtga gcgcgtcgag ggtgacgaga gcggcgacct cggcgcgcgt cagctcgaag
     3781 cccctctcgg cgagggagcg aacaacgcgc tcggggtcgg cccgaaactc acgccggaag
     3841 tcctcgtccg tgacgagcct cccgacgatc cgttccacgt tgccctgcga catccgcttg
     3901 cgtcctctcg actcccgtcg ttacaagtcc cgtgccagac ccgttcccca acggcccgtc
     3961 gacgccgggc cgcatccttg gcggaccgcg acgtggcgcg tcggccccgt gccggttctc
     4021 acggtgcgga gaaggcggca gtctgtagaa ttcgccgcat cgcgaaacgg cgggcggccc
     4081 tgacgacgtc ggacgggacc gcccctcgcg aaggaggaac cgtgaacgtg aaggcgaccc
     4141 gaatcctcgt cgtcgcgggg gcctgcgccc tcgtcttctc cacagcctgc ggcaagaagg
     4201 agacgcccga gagcgagggg aaggagctct cgaagaaccc gatcgcggcg atgggccaga
     4261 tccaggaagc cgtcgagaag gcggcgcagg cggcgaagga gtcccaggag atgaaggcgg
     4321 tggacccggt ccacttctcg aagctgatcg agctcctccc gaaggcgccg gccggcttca
     4381 ccgcggacgg cgagcctcgc ggcgagacga cgaacgcgat gggcttcaag gtctccacgg
     4441 ccgagcagag ctactccgcg gaggggaaga gcctccaggt gaagctgacg gacggggcgt
     4501 tcaacgcacc cctctacacg ttcgtgacga tggcctccca gttcgcgcgc gagtcgaccg
     4561 aggggtacga gaagggcgtc acgtacgacg ggaaccccgg cgtcgagaag tggcggaagg
     4621 acggcggaga cgccgagctg aacatggtcg tcgggaagcg cttcttcgtc gaggtccagg
     4681 gctccgggat cacgcccgag ctcgtccgcg aggtctggtc gtcgatcgac cggacgaccc
     4741 tcgccggcct gaagtgaggg gcgcgtgacg aacatcggga ttccgaagga gtgccgtccc
     4801 ggagagttcc gggtcgggat ctcgccagtc ggcgtccgga tgctgaccgg ggccgggcac
     4861 acctgcttcg tccagagcgg cgcgggcgac ggctcgggct tcctggacga acagtaccgg
     4921 gaagccggcg cgacggtcgt ccactcggcc gaagaggcct ggcgtcgcgc ggatctcgtc
     4981 ctgaaggtga gccgcccgac cgtccaggag ctcgagtggc tccagcccgg caagacgctc
     5041 atgggctacc tctacctcgc ggcggcgcat cccggaaagg tcgagctcct cctcgagaag
     5101 aaggtctcgg ccgtcgcgta cgagctgatc cagctcgccg acggatcgct cccggtcctc
     5161 aagccgctca gccagatcgg gggccggatg gcggcacagg tcgccgcccg gctcctccag
     5221 aacgacgcgg gcgggcacgg gcggctcatc ggcggcgtgg ccggcgtccc gccgaccgag
     5281 gtggtcgtcg tcggagccgg cgtggcggga gaggccgccg cgcgggcgtt cctcggcctc
     5341 ggagcccacg tcacggtcct cgacaccgac ctcgcgcgcc tgcagtcgat cgacgagcgc
     5401 ctctccggca agctcgtgac ggtcgtcgcg cacccgttcc acgtctcccg cgccgccgag
     5461 tacgcggacg tcctcgtcgg ggccgtgatg gtcccgggcg agcgggcgcc gatcgtcttc
     5521 acgcgcgcca tgctccgaaa gatgaagccg ggctcgctct tcctcgacct ttcgatcgac
     5581 caagggggct gcgcggagac gtcgcggccg acgacgcacg agtcgccgac ctacgtcgag
     5641 gacggggtcg tccactgctg catcccgaac ctcggcggcg tcgtcgcgcg cacctcgacg
     5701 cacgccttcc tcaacgcggc cgctccgtgg atcgagcggg tcgtcgtcca gggcatcgag
     5761 gccgccgccg cggcggaccc ggccctcgcg cacggcctcg tcacgcacgc gggcgagctc
     5821 cggaacttcc ggcgggtctc ctacgcccgg cccgtcgaat gagacaggaa gcgaacgaac
     5881 cacgatgagc tggctccaca cctaccacga ccggatcgtc agcgccgagg aggccgtccg
     5941 gacggtcaag tcgggcgacc gcgtctacct gaccgggaac tgctccgtcc cgcaggccct
     6001 gatgaaggcc ctgatccaga gggctcccga gctcacggac gtcgagatca cccacatcct
     6061 gacgctcggg aagacgccct acgccgaccc cgaattcgca gggcacatcc gggtgaacac
     6121 gatgttcatc ggggaggggg tccgcggcgc cgtcaacgag gggcgcgcgg acttcacgcc
     6181 ggtccgcctc tccgaggtcc cgaagctctt ctccgagggg atcgtccctc tcgacgtcgc
     6241 tttcatccac gtcacccctc ccgacgagca cgggttctgc tcctacggcg tcgaggtctc
     6301 gacgaatcgg gccgccgcat cggccgcgaa gctcgtcgtc gcggagatca acccgcagat
     6361 gccgcggacg ctcggggacg ccttcatcca catcagccgg atccacagga tcgtcccggt
     6421 cgactacccg atcatcgaga cgaagatggg gacgccgtcg gagctctcga tgcggatcgg
     6481 caagttcgtc gccgacctga tcgaggacgg ctcgacgatg cagatgggga tcggggcgat
     6541 ccccgacggc gtcctctact acctcaagga caagaaggac ctcgggatcc acaccgagat
     6601 gttctcggac ggcgtcgtcg agctctacga gaagggggtc gtcaccggcg agaagaagaa
     6661 catcgaccgg ggcaagatcg tcgcgggctt cctcatcggc tcgaagcggc tctacgactt
     6721 cgtcgacaac aacccggtcg tcgagatgcg cccgacggag tacgtcaacg acccgtacgt
     6781 catccgccag aacgaccgga tggtcgcgat caactccgcg atcgaggtcg acctgacggg
     6841 ccaggtctgc gccgactcga tcgggacgag gctctactcg ggcgtcggcg ggcagctcga
     6901 cttcatccgg ggcgcggccc gctcgcgcgg cggcaagccg atcatcggcc tcccctcgag
     6961 cggcacgggg aaggacaagc aggccttcac gcggatcgtg ccgatgctca agcacggcgc
     7021 cggcgtcgtc acgggccgct acgacgtcca ctacgtcgtc acggagtggg gcgtcgcgaa
     7081 cctctacggg aggacgatcc ggcagcgggc gaaggcgctc atcgacgtcg cccatcccga
     7141 cttccgcgcg gagctcgagc ggaaggcgat cgagctgaag tacatctgag
//
LOCUS       DAOTEZ010000210         7150 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_7485_length_7150_cov_6.404933, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000210 DAOTEZ010000000
VERSION     DAOTEZ010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7150
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7485_length_7150_cov_6.404933"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="P5164_11745"
     CDS             complement(<1..1003)
                     /locus_tag="P5164_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44606.1"
                     /translation="MRPTAPPILLALLLALPAVGSDLEARAAALARVGGASSPSFSPD
                     GSRLAFVSDLSGVPQAWTIPVQGGFPERVTPFDDPVVKVRWSPAGALLAFSVAPGGAA
                     SEQVWVSRPDGTGLRLLTDGGRETNRLDGFTRDASRLRLASSGRNAAAIDAYLLDPLT
                     REVSARYAASRASCFADVSCDGRFALLSRASRSGSDDLLLVDLESGAELLLTPLERPA
                     SVREAMFSPDSRTVWLSTDAGRDLLAFARIRLAPNGAPGPIEVVASRDDAELAAFAVD
                     GAGLTAALVWNVAGRSELSFVDLATLRSRPGPALPAEVATDPVFSPDGARLAVVLSGP
                     "
     gene            1069..2082
                     /gene="pdxA"
                     /locus_tag="P5164_11750"
     CDS             1069..2082
                     /gene="pdxA"
                     /locus_tag="P5164_11750"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRY44607.1"
                     /translation="MPDARPVVAVTLGDPASIGPEVVVKALAGGGLDAVCVPVVVGDR
                     RVVARALSSTGIALEIVPVGRPGEATGEPGRVEVVDVPAYGIDGIAWGRVQATAGRAA
                     YESIVVAIDLALAGEADAVATAPVSKEAFRAAGVTQIGHTEIFAERTGTIEPLTLFET
                     RGLRVFFLTRHLSLAEALRRVTAERVLATLRGAAAALGTLGVAAPRIAVAALNPHGGE
                     HGLFGDEEERELVPAVALAREEGIDASGPFPADSVFWHASEGRFDAVLSLYHDQGHVA
                     AKMYDFERTVSITCGLPFLRTSVDHGTAFDLAGTGRASATSMAEAIRTAARHAFAVRP
                     RPA"
     gene            complement(2083..2703)
                     /locus_tag="P5164_11755"
     CDS             complement(2083..2703)
                     /locus_tag="P5164_11755"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY44608.1"
                     /translation="MGPERDGTKTYDADYYARWYHADPRERAVRSALVRRKAALALAA
                     AEHLLERETRNVLDVGCGEAPWRAALLRLRPRLRYVGVDPSAWAAAKWGRRRGVRHGG
                     LADLDRLRLGGPFDLVVCSDVVHYVGDADLRRGLASMTALARGVLWIEAFTSEDEFEG
                     DVESFRPRSGRAYRAAFRRAGLVPLGLFLHASPALAPRVAALERGG"
     gene            2821..3612
                     /locus_tag="P5164_11760"
     CDS             2821..3612
                     /locus_tag="P5164_11760"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HRY44609.1"
                     /translation="MKPFSRLAAAALLSLPLAAAAQWEGSFDMKISGKDVAGSAKALV
                     GKAGSRMQMEMTPLAAKGAGGMKMTVISRTAEPGVSYVVNDEHRSYARIDVKEMEKQV
                     PAQERERKWTVKRLGPDRVAGYSCERAILAQEGGDTENEVCVTKELGGTAGLFDTGRR
                     AGSGMAVEKTLKANGLDGMPVRMIIRKKGDAQATMTWELVKAEKRPVPASTFEVPAGY
                     KETSMMGTMMSPEQQKQIDDAMKSLTPEQRKMLEQMMKKQGGGGR"
     gene            complement(3685..5055)
                     /gene="mgtE"
                     /locus_tag="P5164_11765"
     CDS             complement(3685..5055)
                     /gene="mgtE"
                     /locus_tag="P5164_11765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRY44610.1"
                     /translation="MRIGELLGPDVLEIVREDPAALREGLLEFHPADVADLLAAIPRE
                     DRIPVLEQLHEEQLGVVLSYLGGGVLRMGLTRLPPAKIAQALDSLEPDDAARLLSFLP
                     EERRLPVLARMSAKDAAAARGLLAWEPGTAGRLMTDRFVKVSPDWSVSETMAHLRKID
                     PEVATVADLYAVDEKGRLVGVTSLRKLFPAEPGRKVWELMTHDVISVAPDTPQEEVAR
                     LVAKYGFNAMPVVGEDGKALGVVTVDDVVDVLVARETEAALAMGAVAAPSDPDEKHEF
                     EYFGAPLWRVVKSRLGWLLLLFIAGTLTGSVLKHFEGELSRVVALSFFIPLIIGTGGN
                     AGSQTVSTIIRAMALKQVKVKDALRVCLREMSSGLILGGILCAVAVGRVSLWGVGGSL
                     ALTVGLTILAVCVWANAIAALVPLLAERFDFDPTVASAPLIATLVDATGLAIYMVIAK
                     AVLPEI"
     gene            5186..6613
                     /locus_tag="P5164_11770"
     CDS             5186..6613
                     /locus_tag="P5164_11770"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY44611.1"
                     /translation="MNGEDGRVLVVDDDPVNRAILVGSLEAQGYEAGTASEGREALRM
                     LRTGAFDVVLLDLVMPVMDGFEVLRTMKSDERLRSIPVIVVSALEEMESVVRCIEMGA
                     ADYLAKPFDPVLLRARLRASLASKRLDDVVAAHLLEIEELAEQLKSKNRFIQETFGRY
                     LSEAVVAELLDSPDGLRLGGEKRKVTMLMSDLRGFSAIAERLEPEQVVRVINNYLGTM
                     ADVILAWDGTIDEFIGDSVLGFFGAPVARPDDARRALACALAMQKGMEDVNARNRSEG
                     LPAVEMGIAVHTGEVVVGNIGSEKRAKYGAVGSQVNLLARIEAFTCGGQVLISERTLQ
                     EAGHGIVTGMSLTVRAKGFPEPLRAHDLLGLVEAPHLHLGQRADELRPLEPPLKAVFT
                     VLEGKHVARVEEPAEIVALSARGAALRTSASIEVLSDLKLRIVGAGGLLPGELYAKVL
                     PSPGGDLVRLRFTSVPVELESMLSP"
     gene            6650..>7150
                     /locus_tag="P5164_11775"
     CDS             6650..>7150
                     /locus_tag="P5164_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin RelE"
                     /protein_id="HRY44612.1"
                     /translation="MARPPRLEYPGALWYVSARGAARAEAFLDDDDRAAFVPLLARTA
                     STYGWRLHAWALAARSYDLLVETPEPTLSRGMRDLNGVTTQRFNRRHGRSGPLFDGRY
                     RAFLVEREANLLSLVRHVLRAPVREGLAAAPAEWPWSSVRATAGLVEAPRWLVTDATL
                     GALGGRP"
BASE COUNT         1028 a         2468 c         2747 g          907 t
ORIGIN      
        1 cgggaccgga gaggacgacg gcgagccgtg cgccgtccgg ggagaagacg ggatcggtgg
       61 cgacctcggc cgggagggcc ggcccgggcc ggcttcgcag ggtggcgagg tcgacgaacg
      121 agagctcgct ccgtccggcg acgttccaga cgagcgcggc ggtgagcccc gcgccgtcga
      181 cggcgaacgc ggcgagctcc gcgtcatcgc gggaggcgac gacttcgatc ggaccggggg
      241 cgccgttcgg cgcgaggcgg atccgggcga aggcgaggag gtcgcggccg gcatcggtcg
      301 agagccagac ggtcctcgag tcgggcgaga acatcgcctc gcggacggac gccgggcgct
      361 cgagcggggt caggaggagc tcggctccgc tctcgaggtc gacgaggagg aggtcgtcgc
      421 tcccggaacg ggacgcgcgg gagaggagcg cgaaacggcc gtcgcaggag acgtcggcga
      481 agcagctcgc tcgcgaggcg gcgtaccggg ccgagacctc gcgcgtgagc gggtcgagga
      541 ggtaggcgtc gatcgccgcg gcgttccgcc cgctggaggc gagccggagc cgggaagcgt
      601 cgcgagtgaa gccgtcgagc cggttcgtct cgcggccgcc gtcggtcaga agccggaggc
      661 cggtcccgtc ggggcgcgag acccagacct gctcgctggc ggcgcctccc ggcgcgaccg
      721 agaacgccag gagcgcgccg gcgggcgacc agcggacttt cacgaccggg tcgtcgaacg
      781 gcgtcacgcg ctccgggaag ccgccctgga ccgggatcgt ccaggcctgc gggacgccgg
      841 agaggtccga tacgaacgcg aggcgcgagc cgtcgggcga gaacgacggt gacgaagcgc
      901 cgccgacgcg ggccagcgcc gcggcgcgag cttcgagatc gctcccgacg gcagggaggg
      961 cgaggaggag ggcgaggagg atcggcgggg cggtcgggcg catgctcacc tcgggcggtt
     1021 ctgccggcgt ggcgtcgtca tgggatcatc cctctggaag cgaaagcgat gcccgatgct
     1081 cgacctgtcg tcgccgtgac cctcggggac cccgcctcga tcggcccgga ggtggtcgtg
     1141 aaggcgctgg ccgggggagg cctggacgcc gtctgcgtac ccgtcgtcgt cggagaccgg
     1201 cgcgtcgtcg cgcgggcact ctcgtcgacg gggatcgcgc tcgagatcgt ccccgtcggg
     1261 cgccccggag aggcgacggg ggagcccggg cgagtcgagg tcgtcgacgt ccccgcctac
     1321 ggcatcgacg ggatcgcctg ggggcgggtc caggccacgg ccgggcgcgc cgcgtacgag
     1381 tcgatcgtcg tggccatcga cctcgccctc gccggcgagg ccgacgccgt cgcgacggcc
     1441 cctgtgagca aggaggcctt ccgcgcggca ggcgtcaccc agatcgggca cacggagatc
     1501 ttcgcggagc ggaccgggac gatcgagccg ctcacgctct tcgagacgag agggctgcgg
     1561 gtcttctttc tcacgagaca cctctcgctc gccgaggcgc tccgaagggt cacggcggag
     1621 cgtgtcctcg cgacgctgcg cggcgccgcc gcggcgctcg ggacgctggg cgtcgccgcg
     1681 ccgcggatcg ccgtggccgc gctgaaccct cacggcggcg agcacgggct cttcggcgac
     1741 gaggaggagc gtgagctggt cccggccgtg gcgctcgcgc gggaggaggg gatcgacgcg
     1801 tccgggccgt tccccgccga ctcggtcttc tggcacgcgt ccgaggggcg cttcgacgcg
     1861 gtcctttcgc tctaccacga ccaggggcac gtcgccgcga agatgtacga cttcgagcgc
     1921 accgtctcga tcacctgcgg cctgcccttc ctcaggacct ccgtggacca cgggacggcc
     1981 ttcgacctcg ccgggacggg gcgcgcgagc gcgacgagca tggcggaagc gatccggacg
     2041 gcggcgcggc acgccttcgc ggtccgtccg cggccggcct gatcagccgc cgcgctcgag
     2101 cgccgcgacg cggggcgcga gcgccggcga ggcatggaga aagaggccga gcgggacgag
     2161 gccggctcgg cggaaggcgg cccggtacgc gcggccgctc cgcggccgga agctctcgac
     2221 atccccctcg aactcgtctt ccgaggtgaa cgcctcgatc cagaggacgc cgcgggcgag
     2281 cgccgtcatc gaggcgaggc cgcgcctcag gtcggcgtcc ccgacgtagt ggacgacgtc
     2341 ggagcagacg acgaggtcga acggcccgcc gagccggagg cggtcgaggt cggcgagccc
     2401 gccgtgccgg acgccccgcc tccggcccca cttcgccgcg gcccacgcgc tcgggtcgac
     2461 gccaacgtag cggagccggg gccggaggcg gagaagagcg gcccgccacg gcgcctcccc
     2521 gcagccgacg tcgaggacgt tccgggtctc cctctcgagg aggtgctcgg cggcggcgag
     2581 cgcgagcgcc gccttgcgcc tcacgagggc gctgcggacg gcgcgctccc gcgggtccgc
     2641 gtggtaccac cgggcgtagt agtcggcgtc gtacgtcttc gtcccgtcgc gttccggccc
     2701 catgcgcgca ttctgcccgc ggagcgggga gtgcgggggc gccctaccgg cggccgcggc
     2761 gggggtagac tgcccgtccg agatcacagc cccggacgtc atccgcgaaa ggagcccccc
     2821 gtgaagccgt tctcgcgcct cgccgccgcc gctctcctct ccctcccgct cgccgccgcc
     2881 gcgcagtggg aaggctcgtt cgacatgaag atctccggca aggacgtcgc cgggagcgcg
     2941 aaggcgctcg tcgggaaggc cggctcccgg atgcagatgg agatgacccc gctcgcggcg
     3001 aagggggcgg gcggcatgaa gatgaccgtc atctcccgga ccgccgagcc gggcgtcagc
     3061 tacgtcgtca acgacgagca ccggagctac gcccgtatcg acgtgaagga gatggagaag
     3121 caggtccccg cgcaggagcg ggagcggaag tggaccgtga agcgcctcgg gccggaccgg
     3181 gtcgccggct actcctgcga gagggcgatc ctcgcccagg agggcggcga caccgagaac
     3241 gaggtctgcg tgacgaagga gctcggcggc acggcggggc tcttcgacac ggggcggcga
     3301 gcgggctcgg ggatggcggt cgagaagacg ctgaaggcga acggcctcga cgggatgccc
     3361 gtgcggatga tcatccgcaa gaagggggac gcccaggcga ccatgacctg ggagctcgtg
     3421 aaggcggaga agcgccccgt ccccgcctcg acgttcgagg tccccgccgg ctacaaagag
     3481 acgagcatga tggggacgat gatgtccccc gagcagcaga agcagatcga cgacgcgatg
     3541 aagagcctga cgccggagca gcggaagatg ctcgagcaga tgatgaagaa gcaggggggc
     3601 ggcggccgct gacgccgcgg cgcctcccgc ccgggccccg cgacgccgcc ggccgatccg
     3661 gcggcgtccg cgtctcctcc ccgctcagat ctcgggcagg accgccttcg cgatgaccat
     3721 gtagatcgcg aggccggtcg cgtcgacgag cgtcgcgatg agcggagcgg aagcgacggt
     3781 cgggtcgaag tcgaagcgct cggcgaggag ggggacgagc gcggcgatcg cgttcgccca
     3841 gacgcagacg gccaggatcg tcaggccgac cgtcagcgcg aggctccccc cgacgcccca
     3901 gagcgagacg cgtccgacgg cgacggcgca gaggatcccc ccgaggatca gcccggagct
     3961 catctcccgg aggcagaccc ggagcgcgtc cttcaccttc acctgcttca gggccatcgc
     4021 gcggatgatc gtggagaccg tctggctccc cgcgttgccg cccgtgccga tgatgagagg
     4081 gatgaagaac gagagcgcga cgaccctcga gagctcccct tcgaagtgct tcaggaccga
     4141 gccggtgagt gtcccggcga tgaagaggag gaggagccag ccgagccggc tcttgacgac
     4201 ccgccagagg ggcgctccga agtactcgaa ctcgtgcttc tcgtccgggt cgctcggcgc
     4261 ggcgacggcg cccatcgcga gggcggcctc ggtctcgcgc gcgacgagga cgtcgacgac
     4321 gtcgtccacg gtgacgacgc cgagcgcctt cccgtcctcg ccgacgaccg gcatcgcgtt
     4381 gaagccgtac ttggcgacga ggcgcgcgac ctcctcctgc ggcgtgtccg gcgcgacgga
     4441 gatgacgtcg tgagtcatca gctcccagac cttccggccg ggctccgcgg ggaagagctt
     4501 ccggagcgac gtgacgccga cgagccgccc cttctcgtcg acggcgtaga ggtcggcgac
     4561 cgtcgcgacc tcggggtcga tcttccggag gtgcgccatc gtctcggaga cgctccagtc
     4621 ggggctcacc ttcacgaacc ggtcggtcat gagtcgtccg gcggtccccg gctcccaggc
     4681 gaggaggccc cgcgcggctg cggcgtcctt cgccgacatc cgcgcgagga ccgggaggcg
     4741 ccgctcttcg ggaaggaacg agaggagccg ggcggcgtcg tcgggctcga gcgagtcgag
     4801 cgcctgggcg atcttcgcgg gcgggagccg ggtgaggccc attcgcagga cgccgccgcc
     4861 gaggtacgag aggacgactc cgagctgctc ctcgtgcagc tgctcgagga cggggatccg
     4921 atcctcccgg gggatcgcgg cgaggaggtc ggccacgtcg gcggggtgga actcgagcag
     4981 cccctcgcgg agggccgcgg ggtcctcgcg gacgatctcg aggacgtcgg gaccgagaag
     5041 ctctccgatg cgcaccggag gcgagtttag tccggacggg cgggcctaga atgtccgtgc
     5101 gacggcggac ccggacggac gcgttgcgcg ggcgacggcg ggcgtcccgg gccgcggagg
     5161 gacggatggc cgagcggggg gagacgtgaa cggcgaggac ggccgcgtcc tcgtcgtcga
     5221 cgacgacccg gtcaaccggg cgatcctcgt cgggagcctc gaggcccagg ggtacgaggc
     5281 cgggacggcg tccgaggggc gcgaggcgct ccggatgctc cggacgggcg cgttcgacgt
     5341 cgtcctcctc gacctcgtca tgccggtgat ggacgggttc gaggtcctcc ggacgatgaa
     5401 gtccgacgag cgcctccgat cgatcccggt catcgtcgtc tccgcgctcg aggagatgga
     5461 gagcgtcgtc cgctgcatcg agatgggggc ggccgactac ctcgcgaagc cgttcgaccc
     5521 ggtcctcctc cgcgcccgcc tccgggcctc tctcgcatcc aagcggctcg acgacgtcgt
     5581 ggccgcccac ctgctcgaga tcgaggagct cgccgagcag ctcaagtcga agaaccgttt
     5641 catccaggag acgttcggcc gatacctctc ggaggccgtc gtggccgagc tcctcgactc
     5701 gcccgacggg ctccggctcg gcggcgagaa gcggaaggtc acgatgctca tgtcggacct
     5761 gcgcggcttc tcggcgatcg ccgagcgtct cgagccggag caggtcgtga gggtcatcaa
     5821 caactacctc ggcacgatgg ccgacgtgat cctcgcctgg gacggcacga tcgacgagtt
     5881 catcggggac tccgtcctcg gcttcttcgg cgccccggtc gcccggccgg acgacgcccg
     5941 gcgcgcgctg gcgtgcgccc tggcgatgca gaaggggatg gaggacgtga acgcgaggaa
     6001 ccggagcgag ggcctccccg ccgtcgagat ggggatcgcc gtccacacgg gcgaggtcgt
     6061 cgtggggaac atcggctcgg agaagcgcgc gaagtacggc gccgtcggga gccaggtgaa
     6121 cctcctcgcg aggatcgagg cgttcacgtg cggaggacag gtcctcatct cggagcggac
     6181 gctgcaggag gcggggcacg gcatcgtgac cgggatgtcg ctcacggtgc gcgcgaaggg
     6241 tttcccggag cctctccggg cccacgacct cctcgggctc gtcgaggcgc cccacctcca
     6301 cctggggcag cgggcggacg agctccggcc gctcgaaccg ccgctgaagg cggtcttcac
     6361 cgtcctcgag gggaagcacg tcgcgcgcgt cgaggagccg gccgagatcg tcgcgctctc
     6421 ggcccgcggc gccgcgctcc ggacgagcgc ttcgatcgag gtgctctccg acctgaagct
     6481 ccgcatcgtc ggcgcgggag ggctcctgcc gggcgagctc tacgcgaagg tgcttccgtc
     6541 gcccgggggc gacctcgtcc ggctccgatt cacgtcggtt cccgtggagc tcgagtcgat
     6601 gctctctccg taggggtcag gtctcgattc gctgccctag aatgaggccg tggcgagacc
     6661 gccgaggctg gagtacccgg gcgccctctg gtatgtctcg gcccgcggcg ccgcccgcgc
     6721 cgaggccttc ctcgacgacg acgaccgtgc ggcgttcgtc ccgctcctgg cccgcaccgc
     6781 gtcgacgtac ggctggcggc tccacgcctg ggcgctcgcg gcccgctcgt acgacctgct
     6841 cgtcgagacg ccggagccga cgctctcgcg cgggatgcgc gacctgaacg gcgtcacgac
     6901 gcagcgcttc aaccgccggc acggacggtc gggccccctt ttcgacgggc gctatcgcgc
     6961 tttcctcgtc gagcgcgagg cgaatctcct gtccctcgtg cggcacgtcc tccgcgctcc
     7021 ggtgcgggaa ggcctcgccg cggcgcccgc ggagtggccc tggtcgagcg tccgggcgac
     7081 cgccggcctc gtcgaggcgc cgaggtggct cgtcacggac gcgacgctgg gggcgctggg
     7141 cgggcgtccc
//
LOCUS       DAOTEZ010000211         7145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_7490_length_7145_cov_5.241608, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000211 DAOTEZ010000000
VERSION     DAOTEZ010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7145
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7490_length_7145_cov_5.241608"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="P5164_11780"
     CDS             <1..651
                     /locus_tag="P5164_11780"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44613.1"
                     /translation="AIAREAGLPNWDKPDSQEICFVPASDGPAGFIRREAERLGFELP
                     SHPAASPGDLVRADGTPLGAHPGTLGYTVGQRRGLGVAAGEPLYVVGIRAGERRLVVG
                     SGEELLSRVVPLRDVNLQVPPAGASFPVLARIRSRHPDVRARLELRDPGLRQGGRACL
                     LFEEPVRAAAPGQSAVFYDPDRPELLLGGGRIDRPSGAGEPGAGGGDRGCTEGGTV"
     gene            748..1524
                     /locus_tag="P5164_11785"
     CDS             748..1524
                     /locus_tag="P5164_11785"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HRY44614.1"
                     /translation="MTKTASPPVASLLKDIRAALEERVDDKARLAPAARGGLKAIGAR
                     AGSVKAVAREIAVRHKATLDYSLAAELLDAAASRKVREEILVAVELLDRFRKDFEPAL
                     LLRMDKWSSVADDLDVAEALGEKVAAFVLALDPSKITTIRKWARLRSVGRKRLALLAA
                     AGLVSEGRRDAALALELCEILLNEEHPVLVREVASLLRTTTKIDAKAVQDFLFRRSID
                     GNPAILRAGSENLDAARRAALIAKLEAQQATLGALAAAGR"
     gene            complement(1621..2112)
                     /locus_tag="P5164_11790"
     CDS             complement(1621..2112)
                     /locus_tag="P5164_11790"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRY44615.1"
                     /translation="MTPTTRLAAAFLLSVAPLSLAVEPPGRQDLPIYDTTSVGALAIE
                     AASNAATESGRRLFVNFGTNDCAPCRVVNDAIYEEPFLSAFFKQFIPVFVDVAPGSPN
                     RALLERYGIDPAKGFPALVLSNESLKVAEVTKAGEAAALGAKGKDEVRAWLLKRFKAD
                     SPK"
     gene            complement(2137..2463)
                     /locus_tag="P5164_11795"
     CDS             complement(2137..2463)
                     /locus_tag="P5164_11795"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HRY44616.1"
                     /translation="MAEVPPKGEGAAPPALQFSMDDSVANGVYANFVNIIHNPAEFVF
                     DFARAVPGRNDVRILSRVLTTPVHAKQLLNALAQNIAVYEKSYGPIRVDFEAPRPGNG
                     APVRPN"
     gene            2581..3480
                     /locus_tag="P5164_11800"
     CDS             2581..3480
                     /locus_tag="P5164_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY44617.1"
                     /translation="MQRTTRGVSVLPILVSFLLVPPPAAAAGPSAISAADAAWSRRAA
                     GATGGAASEAAAREVVAAARAAVAAEPESVGARWRLMRALYFLGEHATAGEAAKRTVF
                     EEGKKVGEETLGLLRRQAGAAAARDLSRATPVELAPHLSAVPDAVPAFLWAGVNWGKW
                     ALVFGKGAAARQGAAAKIRDYAEAVVALDPAWDEGGGYRVLGRLHHQTPSIPFVTGWA
                     SRSEALASLRRAVEMGPQNFVNRLYLAEAIWDYEKGRREEARRMLEALLREAPTPGLE
                     VEAARAQEDARALLAAWAGKGRR"
     gene            3477..4397
                     /locus_tag="P5164_11805"
     CDS             3477..4397
                     /locus_tag="P5164_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020467017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HRY44618.1"
                     /translation="MKERIRSAMARIVASAESRPQLSAVEAAPRPQKKEIIEILRLFQ
                     EVVYPGYFGDTILYRDNLRDHLGDVLFRIHLRLSTEVEKALEASEGKPAARRHAREVV
                     TELFERLPEVADRVAEDVQAAYDGDPAAQGFDELILAYPGVKAIFTYRIAHELHRMGV
                     RLIPRVMTEFAHNETGVDIHPGATIGRSFFIDHGTGVVIGETTTIGDRVKIYQGVTLG
                     ALSFPKNEHGELIRGVKRHPTVEDDVVIYAGATILGGSTVIGRGSVIGGSVWLTTSVP
                     AFTRVTLAGEQLRFEPRTPPARGRGPDAPA"
     gene            complement(4401..4880)
                     /gene="folK"
                     /locus_tag="P5164_11810"
     CDS             complement(4401..4880)
                     /gene="folK"
                     /locus_tag="P5164_11810"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017518920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRY44619.1"
                     /translation="MIGLGGNVGSPRLAFETALGALAPCVGGLVLSPLYRTAPVGGPP
                     QPDFLNAVAVGRTSLAPVALLDVLHRIEADAGRRHTPLRDGPRPLDLDLLLYGDLVLE
                     LPGLVLPHPRMASRRFVLAPLADLRPDLVVPGTGRTVSALLAAAPPARVERLSTGER"
     gene            complement(4897..5220)
                     /locus_tag="P5164_11815"
                     /pseudo
     CDS             complement(4897..5220)
                     /locus_tag="P5164_11815"
                     /inference="COORDINATES: protein motif:HMM:NF021083.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FYDLN acid domain-containing protein"
     gene            5377..6510
                     /gene="tgt"
                     /locus_tag="P5164_11820"
     CDS             5377..6510
                     /gene="tgt"
                     /locus_tag="P5164_11820"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRY44620.1"
                     /translation="MLSFELLSADGAARRGRVTLDHGTVETPAFMPVGTAGTVKAVRW
                     SDVSAAGAEIVLANTYHLMLRPGGELIREMGGLHAFVGWDRPILTDSGGFQVMSLAAN
                     RKLTEEGVLFRSHLDGSAHLLTPERAAELQLRDFGVDVAMALDECTPYPATRDEAARS
                     MSLTHRWARRGRAAFLAARGETGRGAQFGIVQGGMHADLRRESAAAVAAEGFEGVACG
                     GVSVGEPKEEMRAIVEATGPLLPPERPRYLMGVGHPGDLLHAVASGFDLFDCVLPTRA
                     ARHGLAYTSEGTVVLKQARFRTDRAPLDPACACPTCATASRAYLRHLFMLGEPTAATL
                     VTVHNLHAILDLMRGIREALAARRYAAFAVEAAGRLDFGKDNR"
     gene            6507..6848
                     /gene="yajC"
                     /locus_tag="P5164_11825"
     CDS             6507..6848
                     /gene="yajC"
                     /locus_tag="P5164_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRY44621.1"
                     /translation="MNPIALLQAAPAAPSMKDGLLQMVPFLLIFVVFYFVLLAPMRKQ
                     QKKTKEMLAQLKKGDRVMTSGGIYGTVAQVEDQIVWVKIADTLKVKMAKSAITGVVAD
                     TEGGKDQGSAA"
     gene            6877..>7145
                     /locus_tag="P5164_11830"
     CDS             6877..>7145
                     /locus_tag="P5164_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44622.1"
                     /translation="MAPLVLLQSSDWHVGSPLTGRGLGLGEELRALRRREVDEAPERL
                     LRAAPQSLRKGVIVNLLSPNPYLFWMTVGAPAMRRRKRPSRRSNS"
BASE COUNT          995 a         2466 c         2691 g          993 t
ORIGIN      
        1 gcgatcgccc gggaggcggg gcttccgaac tgggacaagc ccgactcgca ggagatctgc
       61 ttcgttccgg cctcggacgg tccggcgggc ttcatccgaa gggaagcgga aaggctcggt
      121 ttcgagcttc cgagccaccc ggcggcctct ccgggcgacc tcgtgagagc ggacggcacg
      181 cccctcggag cgcatccggg gacgctcggc tacacggtgg ggcagcgccg ggggctggga
      241 gtcgccgcgg gcgagccgct ctacgtcgtc gggatccgcg ccggagagag gcggctcgtg
      301 gtcgggagcg gcgaggagct cctttcgcgc gtcgtgcctt tgcgggacgt gaacctccag
      361 gtcccgccgg ccggcgcgtc gtttcccgtc ctcgcgcgga tccggtcccg gcacccggac
      421 gtgcgggcgc ggctcgagct ccgggacccc ggccttcgtc agggggggcg ggcctgcctg
      481 ctgttcgagg agccggtccg cgcggcggcg ccgggccagt cggccgtctt ctacgacccg
      541 gaccggcccg agctcctcct cggcggaggc cggatcgaca ggccatcggg agccggggag
      601 ccgggcgccg gaggcggcga tcggggttgc acggaggggg gcacggtgta gcatcggccg
      661 gggaagcggg tggccgccgg gtgcgcgggc ccgtgcgcga ggtgtccaag cggtcgccga
      721 cgtgataaac tctcgcgtcg tccgcgtatg accaagaccg cctccccgcc cgtcgcctcc
      781 ctcctcaagg acattcgagc cgctctcgag gagagggtgg acgacaaggc gcgcctcgcc
      841 cccgctgccc ggggagggtt gaaggcgatc ggcgctcgcg cgggcagcgt caaggccgtg
      901 gcccgcgaaa tcgcggtccg ccacaaggcg acgctcgact actccctggc cgcggagctg
      961 ctcgacgcag cggcttcccg gaaggtgcgc gaggagatcc tcgtcgcggt cgagctgctg
     1021 gaccgcttcc gcaaggattt cgagccggcg ctgctcctcc ggatggacaa gtggagctcc
     1081 gtcgccgacg acctcgacgt ggccgaggcc ctcggagaga aggtggcggc gttcgtcctc
     1141 gcgctcgacc cgtcgaagat cacgacgatc cgcaagtggg cgcggctccg gagcgtcgga
     1201 cggaagcgcc tcgcgctcct ggccgccgcg gggctcgtct ccgaagggcg gcgcgacgcg
     1261 gcgctcgccc tcgagctctg cgagatcctc ctgaacgagg agcatcccgt cctggtccgg
     1321 gaggtcgcga gcctcctgcg gacgacgacg aagatcgacg cgaaggccgt tcaggacttc
     1381 ctgttccggc gctcgatcga cgggaacccc gcgatcctcc gggccggctc ggagaacctc
     1441 gacgcggccc gccgcgccgc gctcatcgcc aagctcgagg cgcagcaggc gaccctcggc
     1501 gccctggccg ccgccgggcg ctgagctccg cggcctcccc ggcctcgaac gcctcccgga
     1561 agggcgcccc cgaccgggag ccggcgcccg aaggcgccgc ctcccgatcg gctcggccgg
     1621 ctacttcggc gagtcggcct tgaatcgctt cagcagccag gcgcggacct cgtccttccc
     1681 cttcgcgccg agggccgcgg cctcgccggc cttcgtcact tccgcgacct tgagagattc
     1741 gttggagagg acgagggccg ggaagccctt ggcggggtcg atcccgtagc gctcgaggag
     1801 cgcccggttc ggcgagcccg gcgcgacgtc gacgaagacg gggatgaact gcttgaagaa
     1861 cgccgagagg aagggctcct cgtagatcgc gtcgttcacg acgcggcacg gagcgcagtc
     1921 gttcgtcccg aagttcacga aaaggcgccg gccggactct gtggcggcgt tcgaggcggc
     1981 ttcgatcgcc agggcgccga cgctggtcgt gtcgtagatc ggcaggtcct gccgcccggg
     2041 cggctcgacg gcgagggaga gcggcgcgac ggagaggagg aatgcggcgg cgagacgggt
     2101 ggtcggtgtc atctttccct ttccgagagc gggcgttcag ttcggtcgga ccggagcgcc
     2161 gttcccgggc cgcggggcct cgaagtcgac gcggatcggg ccgtagctct tctcgtagac
     2221 ggcgatgttc tgcgcgaggg cgttgaggag ctgcttggcg tggacgggcg tcgtgaggac
     2281 ccgggagagg atcctcacgt cgttgcgccc gggaacggcc cgggcgaagt cgaagacgaa
     2341 ctcggccggg ttgtggatga tgttgacgaa gttcgcgtag acgccgttcg cgacggagtc
     2401 gtccatcgag aactggagcg ccgggggagc ggccccttcg cccttgggcg ggacttcagc
     2461 cattgagaca cctcacgatg cgcatcgtcc ggggatgcgc aagaagggtg ccattctagc
     2521 cgtccgctcg cgcgcacgcg gaccggatcc tcgggccggt ggcgaacgta agatccgagg
     2581 atgcagcgca cgacccgggg cgtatcggtc ctcccgatcc tcgtctcctt tctcctcgtc
     2641 ccgccgccgg cggccgccgc ggggccttcc gcgatctcgg ccgccgatgc ggcctggtcg
     2701 cgcagagcgg ccggggcgac gggcggagcg gcttcggagg ccgcggcgcg ggaggtcgtg
     2761 gctgcggccc gggccgccgt tgccgccgag cccgagtcgg tcggggcacg gtggcggctg
     2821 atgcgggcgc tctacttcct cggggagcac gccacggccg gcgaggcggc gaaacggacc
     2881 gtcttcgaag agggaaagaa ggtcggagag gagacgctcg ggctcctgcg gcggcaggcc
     2941 ggcgcggctg cggcgcggga cctctcgcgc gccacgccgg tggagctcgc gccgcacctg
     3001 tcggcggtgc cggacgcggt tccggcgttc ctctgggccg gggtgaactg ggggaagtgg
     3061 gcgctcgtct tcgggaaggg agccgcggcc cgccaggggg ccgccgcgaa gatccgagac
     3121 tacgccgagg ccgtcgtggc gctcgatccg gcctgggacg aagggggcgg gtaccgcgtc
     3181 ctcggtcgcc tccaccacca gactccttcg attcccttcg tcaccgggtg ggcgtcccgg
     3241 agcgaggcgc tggcgagcct tcggcgagcc gtggagatgg gtccgcagaa cttcgtgaac
     3301 cgcctctacc tcgccgaggc gatctgggac tacgagaagg ggaggaggga ggaggcgcgc
     3361 cggatgctcg aggcgctcct ccgcgaggcg ccgacgccgg gtctcgaggt ggaggccgcg
     3421 agagcccagg aggacgcgcg ggcccttctc gccgcatggg ccggaaaggg gcggcgatga
     3481 aagagcggat ccggagcgcg atggcgcgca tcgtcgcttc cgcggagagc cgcccgcagc
     3541 tctccgccgt ggaggccgct ccccggcccc agaagaagga gatcatcgag atcctccggc
     3601 tcttccagga ggtcgtctac ccggggtact tcggtgacac gatcctctac cgggacaacc
     3661 tgcgcgacca cctcggcgac gtcctcttcc ggatccacct ccggctctcc acggaggtgg
     3721 agaaggcgct cgaggcgtcc gaggggaagc cggcggcgcg ccgccacgcg cgcgaggtcg
     3781 tcaccgagct tttcgagagg ctccccgagg tcgccgaccg ggtcgccgag gacgtccagg
     3841 ccgcctacga cggcgaccct gccgcccagg ggttcgacga gctgatcctc gcctacccgg
     3901 gggtgaaggc catcttcacg taccggatcg cgcacgagct gcaccggatg ggggtgcggc
     3961 tgatcccccg ggtcatgacg gagttcgcgc acaacgagac cggcgtcgac atccatcccg
     4021 gcgcgacgat cggacgatcg ttcttcatcg accacgggac gggcgtcgtc atcggcgaga
     4081 cgacgacgat cggggaccgg gtgaagatct accagggcgt gacgctcggg gcgctctcgt
     4141 tccccaagaa cgagcacgga gagctgatcc gcggcgtcaa gcgccatccg acggtggagg
     4201 acgacgtcgt catctacgcg ggggcgacga tcctcggcgg ctccaccgtg atcggacgcg
     4261 gcagcgtgat cggcgggagc gtctggctga cgacctcggt gccggccttc acgcgtgtga
     4321 cgctcgcggg ggagcagctc cggttcgagc cgcggacgcc gcccgcgcgg ggcagggggc
     4381 cggacgctcc ggcctgaggc tcagcgctcc ccggtcgaca ggcgctcgac ccgcgccggc
     4441 ggagccgcgg cgaggagggc cgagaccgtc cgcccggtcc cgggcacgac gaggtcgggc
     4501 cggaggtcgg cgaggggcgc gaggacgaaa cgccggctcg ccatcctcgg gtgcgggagg
     4561 acgagcccgg ggagctcgag gacgaggtcg ccgtagagga ggaggtcgag gtcgagcggc
     4621 cgcggtccgt cgcgcagggg ggtgtgcctg cgtcccgcgt ccgcctcgat ccgatggagg
     4681 acgtcgagga gcgcgacggg cgcgaggctc gtcctcccga cggcgacggc gttcaggaaa
     4741 tcgggctggg gcggcccgcc gacgggggcg gtcctgtaga ggggcgacag gacgagaccg
     4801 ccgacgcagg gtgcgagcgc cccgagggcc gtctcgaacg cgagccgggg agatccgacg
     4861 ttcccgccga ggccgatcac gacctcggcc ggctcgtcag tagtcgtctt cgctctccga
     4921 gtcgcccgcc tcgaggctcg gcccctcgcc gagagggagc tcctcgtcgt cgagggtcgc
     4981 cttggcgttc gggtcctcgg gctccgcgcc gaggtcgtcc tcgtcgcggt tcctctcgaa
     5041 gaggtccggg tcggcaaact cgtcgcgctt ggcgcggccg gcggcgatca gcgcggccgg
     5101 cgacgtgtcg cgcgggggcg cgtccttctg gtcggctccg cacttcgggc agaagacggg
     5161 agttctcttc aggtcgtaga acttcgtccc gcagcggaag cactcgtgtt tctggccaag
     5221 atcgggcatt cgctccctca aaaaagacgc cggaacatag catcgggtcc gaggtcggtc
     5281 aacgagatcc ggagacccgg cgcgcgccgc ttacactcgc cccgtgagcc gctccttcct
     5341 cccgctctcc cggcgcttct ccggtgcgga gcccacgtgc tgagcttcga gctcctctcc
     5401 gcggacgggg ccgcgcggcg cggccgcgtg acgctcgacc acgggaccgt cgagacgccc
     5461 gcgttcatgc ccgtcggcac ggccggaacg gtgaaggcgg tccgctggag cgacgtctcc
     5521 gccgccggcg ccgagatcgt cctcgcgaac acgtatcacc tgatgctccg ccccggagga
     5581 gagctgatcc gcgagatggg cgggctccac gcattcgtcg gctgggaccg accgatcctg
     5641 accgattcgg ggggcttcca ggtgatgtcc ctcgcagcga accggaagct gacggaggag
     5701 ggggtcctgt tccggagcca cctcgacggc agcgcgcacc tcctcacccc ggagcgggcc
     5761 gccgagctcc agcttcgcga cttcggggtc gacgtcgcga tggccctcga cgagtgcacc
     5821 ccgtacccgg cgacgcggga cgaagcggcc cgctcgatgt ccctcacgca ccggtgggcg
     5881 cgccgcgggc gagcggcgtt cctcgcggcg cgaggagaga ccggccgggg cgcgcagttc
     5941 ggcatcgtgc agggcgggat gcacgcggac cttcgccgcg agagcgccgc ggccgtcgcc
     6001 gcggaggggt tcgagggggt cgcgtgcggc ggcgtgtcgg tgggggagcc gaaggaggag
     6061 atgcgcgcga tcgtcgaggc caccgggccg ctccttccgc ccgagcggcc gcgctacctg
     6121 atgggcgtcg ggcaccccgg cgacctcctc cacgcggtgg cgagcgggtt cgacctcttc
     6181 gactgcgtcc tcccgacgcg ggccgcgcgt cacgggctcg cctacacctc ggaggggacg
     6241 gtcgtcctca agcaggcgcg cttccggacc gaccgggccc cgctcgaccc ggcctgcgcc
     6301 tgcccgacgt gcgcgacggc gtcgcgcgcc tacctgcgcc acctcttcat gctcggagag
     6361 ccgacggcgg cgacgctcgt gaccgtccac aacctccacg ccatccttga cttgatgcgg
     6421 ggaatccggg aggctctggc ggcacgacgc tacgcggcgt tcgcggtcga agccgccggg
     6481 cgcctcgatt tcgggaagga caacagatga atccgatcgc cctcctgcag gccgccccgg
     6541 cggcgccgtc gatgaaggac ggcctgcttc agatggtccc gttcctcctc atcttcgtcg
     6601 tcttctactt cgtcctcctc gccccgatga ggaagcagca gaagaagacg aaggagatgc
     6661 tcgcccagct gaagaagggc gaccgggtca tgacctccgg cgggatctac gggaccgtcg
     6721 cccaggtcga ggaccagatc gtctgggtga agatcgccga cacgctgaag gtgaagatgg
     6781 ccaagagcgc catcacgggc gtcgtcgcgg acaccgaggg cggcaaggac cagggctcgg
     6841 cggcctgagg ccgcgcggcg cccgagcgcc atcgtcgtgg cgcccctcgt cctcctccag
     6901 agctccgact ggcacgtggg ctcgcccctg accgggcgcg gcctcggcct gggagaggag
     6961 ctccgggcgc tccggcgccg ggaggtcgac gaggcgcccg aacggctcct ccgcgccgcc
     7021 ccgcagtcgc tgcggaaagg cgtcatcgtg aatctgctca gccccaaccc gtacctattc
     7081 tggatgacgg tcggcgcgcc ggcgatgcgc aggaggaagc gcccatcgcg gcgttcgaat
     7141 tcgat
//
LOCUS       DAOTEZ010000212         7140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_7495_length_7140_cov_5.549894, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000212 DAOTEZ010000000
VERSION     DAOTEZ010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7140
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7495_length_7140_cov_5.549894"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="P5164_11835"
     CDS             complement(<1..444)
                     /locus_tag="P5164_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HRY44623.1"
                     /translation="MGLIKDLPDNVITTSVDALIGWGRKNSLWPLQFGLACCAIEMMA
                     VTDSRHDMARFGAEVFRGSPRQADLMLVAGTVTEKMAPIVRRLYDQMADPKWVIAMGS
                     CATCGGPYRTYAVTQGVDRLIPVDVYVPGCPPRPEALLYGLMELQK"
     gene            complement(435..791)
                     /gene="ndhC"
                     /locus_tag="P5164_11840"
     CDS             complement(435..791)
                     /gene="ndhC"
                     /locus_tag="P5164_11840"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRY44624.1"
                     /translation="MQSYIPILFFAAIAALFPIAILVVMALLRAKAPNPTKLSPYECG
                     IASATDAHDHRFSVRYYLIAVLFVVFDVETVFLFPWAVMYDKLLLFGLIEMAVFLYIL
                     VLGYVYVWKRGALSWA"
     gene            complement(894..2000)
                     /locus_tag="P5164_11845"
     CDS             complement(894..2000)
                     /locus_tag="P5164_11845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRY44625.1"
                     /translation="MSDAGVSPLADRLRDRIAREGPLRFDDFQGAALYDAAGGYYERP
                     GRVGRSGDFVTGASWHPAFARCLARATTRLARELGEAIDLVDVGCGEGELLSFLDETA
                     AGAGWRLRGVEASAERRAAALRRVPGATFHSSSDELPGGLTGLVVAYELLDALPVRAL
                     RVEEEGTLSERRVGLDGEGRFTWVEQPCPDGSEILSALASRGAALEPNQLLEVRPGAA
                     ALVRTIASRLDRGLLLFFDYGAPTRALYGPARPNGTLEAFLSHRVSRDVLTDPGSRDI
                     TAWVDFGEVEETLRAEGFVVRGLVSQSRFLLATGVAEELALAPGEAVSASMVAERNAV
                     AKLVSPGGMGESIRVLVAERETGLGKSLISSPLV"
     gene            2070..2483
                     /locus_tag="P5164_11850"
     CDS             2070..2483
                     /locus_tag="P5164_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44626.1"
                     /translation="MPYELKTSPDEIQLLVSSPDRAGLFRDTLAGVLEAVYGAALPEG
                     KNEGQVVPVQASAGDDDALLVELVHDVLRAAREEGGALRMPRWFAFDERRVTANLPIQ
                     LPKVDVRPIQVSGAAVETGEGGWNARVELLPRVEA"
     gene            complement(2571..3062)
                     /locus_tag="P5164_11855"
     CDS             complement(2571..3062)
                     /locus_tag="P5164_11855"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HRY44627.1"
                     /translation="MKRNSKGFTLIELLIVVAIIGIIVAIAIPNLLNAIQRAKQRRSM
                     GDIRTTATAIEAYAVDLNRYPPAAAYTLPIAINQTLSSISNYVSPTYVKQVPLSDGWN
                     SWFMASVDGTGSIYALASAGRDGSATGLAANATLLGPTTDFNADIVYVNGSFIQWPEG
                     VQR"
     gene            complement(3233..3724)
                     /locus_tag="P5164_11860"
     CDS             complement(3233..3724)
                     /locus_tag="P5164_11860"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HRY44628.1"
                     /translation="MRIRRWAGFTLIEVLVVVAIVGIVVAIGVVNYLNAIERARQRRS
                     MADMRGISTAIEAYAADLDRYPPASAFVLPAGLGLPTESLAATQNYLQPTYIRTLPLV
                     DGWNSWFTYGVNASRADYVLRSNGRGGLPQTDPLYEPTTDFRDDIILVNGRFVQWPDG
                     VQH"
     gene            3802..5334
                     /locus_tag="P5164_11865"
     CDS             3802..5334
                     /locus_tag="P5164_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015174641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HRY44629.1"
                     /translation="MIPKRPFVSELTLERELEEFDRLKPRLREVWDSFTMRDEEPHTS
                     IVVPSLTLDQEELRKLEGSPYYEERLLFLLIRLRNPRARVVFVTSQPVHPLVLEYYFQ
                     LLVGIPASHARARLTLLCAYDDSPRSLTEKILERPRLIERIRAGIADPARSYLTVFNS
                     TPLERKLAVLLGVPLNGVDPSLRHLGTKSGSRKVFREAGVPLPEGFEDLRSLQDVEEA
                     LLELHRRRPRARKAVVKLDDSFSGEGNALFRYPSRVERETVREAMQRFEYAVPWETND
                     DYFSRFARMGGVVEEFLEADQKTSPSVQLRVDPHGAVVPISTHDQVLGGVGGQVFLGC
                     LFPAADGYRLEAQAAAHRIGEVLAGYGVVSRFGVDFLATRSDDDSPWSLHALEINLRM
                     GGTTHPYLALQFLSGGRLDPGSGLFYSPRGLPKFYRATDNLKSEAYRGLLPEDLIEIL
                     TINGLHYSHNTESGVLFHLIGALSQYGKLGVTAIGNSREEVEHLYARTLAILDRETSF
                     GR"
     gene            complement(5417..6904)
                     /locus_tag="P5164_11870"
     CDS             complement(5417..6904)
                     /locus_tag="P5164_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44630.1"
                     /translation="MESVRSSRFPRAFALALLVHLAAYLPSLPNGFVFDDHAMVEARP
                     EVTGQAPIREAFLKPWFHEEQVGAGVAYRPAARLSLGLDVRAFGLDPLPLRFSNLLWG
                     ALGAALLGLLVVEVGAPLVVADVVVVLFSAHPARSDVLLAIVGRAELLSFAAVAGALL
                     LALRSAKRTGPGRWGLASASALVLALGLLSKETAFAAPLLLGATVLPGIREGRLRESL
                     SRLGPTAACWGLVGAGVFALRLAALGGFLTGPNARIAPIENKLASGPAAERVSGAVAL
                     VPLAAERLTWPRTLIADYGSNAIADADLRDARRIAAGVAILLGVSGAAFCLRSRAPMT
                     SLGLAWALLSYLPFANVAYPTAVLFTERLLFLPAVGVVFCAGDLVARVPRPGRRAALL
                     AVGLLALAGASRIWSRIPDWRDDRALFAATVRDAPGNGRAWVNLGVLSLARGDAASAT
                     AELTEGLRADPGLRPRVEGMRRHAVGLGRTDLSRAIDSALNASGG"
BASE COUNT         1173 a         2529 c         2454 g          984 t
ORIGIN      
        1 cttctgcagc tccatgaggc cgtagaggag cgcctcgggc cgcggggggc agcccgggac
       61 atagacgtcg acggggatga gccggtcgac tccctgggtc acggcgtagg tccggtaggg
      121 gcctccgcac gtcgcgcagg agcccatggc gatcacccac ttcggatcgg ccatctggtc
      181 gtagaggcgg cggacgatgg gggccatctt ctccgtgacc gtcccggcga cgagcatcag
      241 gtcggcctga cgcggcgagc cgcggaagac ctcggcgccg aagcgcgcca tgtcgtggcg
      301 gctgtcggtc acggccatca tctcgatggc gcagcaggcg aggccgaact gcagcggcca
      361 gagggagttc ttgcgccccc agccgatcag ggcgtcgacc gaggtcgtga tgacgttgtc
      421 cggcaggtcc ttgatcaggc ccacgagagc gcccccctct tccagacgta gacgtatccc
      481 agcacgagga tgtagaggaa gaccgccatc tcgatcagcc cgaacaggag gagcttgtcg
      541 tacatgaccg cccacgggaa gaggaagacc gtctcgacgt cgaagacgac gaagaggacg
      601 gcgatgaggt agtaccggac cgagaaccgg tgatcgtggg catcggtcgc cgacgcgatg
      661 ccgcactcgt agggcgacag cttcgtcggg ttcggcgcct tggcccgcag gagcgccatc
      721 acgacgagaa tggcgatcgg gaagagcgcc gcgatcgcgg cgaagaagag aatcggaatg
      781 tagctctgca cggcctcggc cccccggcgc gcgcgcctcc ccgggggcgc ttccgcccct
      841 ggtttgtgcg cgtcgtcaca tgcggcggat tgtccggtcg ggatgtctcg aagtcaaacc
      901 aaggggctcg aaatcaagga cttcccgagt cccgtttcgc gttcggcgac gagaacgcgg
      961 atcgactccc ccatccctcc cgggctgacg agcttggcca cggcgttcct ctcggcgacc
     1021 atcgacgccg agacggcctc gcccggggcg agcgcgagct cctccgcgac tccggtcgcg
     1081 aggaggaaac gggactgcga gacgagcccg cggacgacga agccttcggc gcggagcgtc
     1141 tcctcgacct ccccgaagtc gacccaggcc gtgatgtcgc ggctccccgg gtcggtgagg
     1201 acgtcgcgcg agacgcggtg ggacaggaac gcctcgaggg tcccgttcgg gcgcgccggg
     1261 ccgtagaggg cccgcgtcgg ggccccgtag tcgaagaaga ggagcagccc ccggtcgagg
     1321 cgggacgcga tcgtccggac gagcgccgcc gcccccgggc ggacctcgag aagctgattc
     1381 ggctccagcg ccgctccccg gctcgcgagc gcggagagga tctcggagcc gtcggggcag
     1441 ggctgctcga cccacgtgaa tctgccctcc ccgtccagac cgacgcgtcg ctcggagagc
     1501 gtgccctcct cttccacccg gagggcgcga acggggaggg cgtcgagcag ctcgtaagcg
     1561 acgacgagcc ctgtgagtcc tcccgggagc tcgtcgctgg aggagtggaa cgtggcgcct
     1621 ggcactcgcc ggagggcggc ggctcgccgt tcagccgagg cctccacgcc acgaagccgc
     1681 cagccggcgc ccgccgccgt ctcgtccagg aacgagagaa gctccccttc tccgcaaccg
     1741 acgtcgacga ggtcgatcgc ctctccgagc tcccgggcaa ggcgggtggt cgcccgcgcg
     1801 aggcaccgcg cgaaggccgg gtgccacgag gccccggtga cgaagtcgcc gcttcggccg
     1861 acgcgaccgg gccgctcgta gtagccgccc gccgcgtcgt agagcgcagc gccctggaag
     1921 tcgtcgaacc ggagcggccc ttctcgagcg atccggtcgc gaaggcggtc ggccaggggg
     1981 gagacgcctg cgtcgctcac ctcctcattc tgaccgtctc cgggtacggc gtcagcgcta
     2041 agatcttcgg ccgcggaagg agacacgccg tgccgtacga gctgaagacg agtccggacg
     2101 aaatccagct tctggtgagc tcccccgacc gcgcgggcct tttcagggac accctggccg
     2161 gcgtcctcga ggccgtctat ggcgcagcgc tcccggaggg aaagaacgag ggacaggtcg
     2221 tccccgtcca ggcatcggcg ggagacgacg acgccctcct cgtcgagctc gtccatgacg
     2281 tcctcagggc ggcacgcgaa gagggagggg cgctccggat gccccgctgg ttcgcgttcg
     2341 acgagaggcg cgtcacggcc aacctcccca tccagctgcc gaaggtcgac gtccggccga
     2401 ttcaggtctc gggagcggcc gtggagaccg gcgagggggg ctggaacgcc cgggtcgagc
     2461 ttctgccacg agtcgaggcc tgaccgggcg gcccctcggc ccgatccgca tgaaaaaagc
     2521 ccccggggcc gaaaccccgg aggctcttca tccaaggatg gtcgagcgcc tcagcgctgg
     2581 acaccctccg gccactgaat gaaggaaccg ttcacgtaga cgatgtcggc gttgaagtcg
     2641 gtcgtggggc cgaggagcgt agcgttcgcc gcgaggccgg tcgccgatcc atcccgacct
     2701 gccgacgcga gggcgtagat gcttccggtg ccgtcgaccg aggccatgaa ccaggagttc
     2761 cagccgtcgg agagcgggac ctgcttgaca taggtcgggg aaacgtagtt cgaaatactg
     2821 ctaagcgtct ggttaatagc gatggggagc gtgtaggcag ccgccggcgg gtaccggttg
     2881 aggtcgacgg catacgcctc gatggcggtc gccgtcgtcc ggatgtcgcc catcgaccgg
     2941 cgctgcttgg cgcgctgaat cgcgttcagc aggttcggga tcgcgatcgc gacgatgatg
     3001 ccgatgatcg cgacgacgat cagcagctcg atgagggtga agcctttcga gttcctcttc
     3061 atgtgagaaa gcctccttgc ctgccgtcgt gcaaactccg cgccgttgtt cgtccgtgga
     3121 cccgagcgcc tacttctgcc ctttcccacc ccgcccgcga gaaagccgac aaaaattgtc
     3181 acaccccgtc gcccccgacc cccgaaaagt gtcggtgcgc ttcgggcccc ggtcagtgct
     3241 gcacaccatc cggccactga acgaaccggc cgttgaccag aatgatgtcg tcacggaagt
     3301 cggtcgtcgg ctcgtaaagg gggtcggtct ggggaaggcc acccctcccg ttcgatctca
     3361 ggacgtagtc ggcccgcgag gcgttcaccc cgtacgtgaa ccaggagttc cagccatcca
     3421 ccagcgggag cgtacgaata taggttggct gaaggtagtt ctgcgtcgcc gccaggctct
     3481 cggtgggaag gcccagaccg gccggcagga cgaaggcgct cgctggagga tagcggtcga
     3541 ggtcggccgc gtaggcttcg atcgccgtcg agatccctcg catgtccgcc atggaccggc
     3601 gctgccgggc ccgttcgatc gcgttgagat agttcacgac cccgatcgcg acgacgatcc
     3661 cgactatggc gacgacgacg aggacctcga tcaaggtgaa tcctgcccac cgtcggatgc
     3721 gcatctttcg gttctcccct tcgaacgatc gaatcaagat ccgtgccgat cccgggggct
     3781 ccccccctag aatgggcggg cgtgatcccg aaacggcctt tcgtgagcga gctgacgctc
     3841 gagcgcgagc tcgaggagtt cgatcgcctc aagccgaggc tccgcgaggt ctgggactcc
     3901 ttcacgatga gggacgagga gccccacacg tcgatcgtcg tcccctccct cacgctggac
     3961 caggaggagc tccggaagct ggagggctcg ccctactacg aggaacgcct cctgttcctc
     4021 ctcattcgtc tccggaatcc ccgcgcgcgc gtcgtcttcg tgacctccca gccggtccac
     4081 ccgctcgtcc tcgagtacta cttccagctc ctcgtcggga tcccggccag ccacgcccgg
     4141 gcccggctga ccctcctctg cgcctacgac gactcgccgc gatcgctgac ggagaagatc
     4201 ctcgagcgcc ctcgcctcat cgagcgcatc cgggccggca tcgccgaccc ggcgcgctcc
     4261 tacctcaccg tcttcaactc gacgccgctc gagcggaagc tcgccgtcct cctcggcgtc
     4321 cctctcaacg gcgtcgatcc ctcgctccgc cacctcggga cgaagtccgg gagccggaag
     4381 gtctttcgcg aggccggcgt cccgctcccg gaaggcttcg aggacctccg gtccctccag
     4441 gacgtcgagg aggctctcct cgagctccac cgccgacgac cccgggcccg aaaggcggtc
     4501 gtgaagctgg acgacagctt ctcgggagaa gggaacgccc tcttccgata cccgtcgagg
     4561 gtggagcggg agaccgtgcg agaggcgatg cagcggttcg agtacgccgt cccgtgggag
     4621 acgaacgacg actacttctc ccgcttcgcg cggatggggg gcgtcgtcga ggagttcctc
     4681 gaggccgatc agaagacctc gccgagcgtc cagctccgag tcgaccctca cggcgccgtc
     4741 gtcccgatct cgacccacga ccaggtgctc ggcggcgtcg gcgggcaggt cttcctcggc
     4801 tgcctcttcc cggcggccga cggctatcgc ctcgaggccc aggcggccgc tcaccgcatc
     4861 ggcgaggtcc tcgcggggta cggcgtcgtc agccgcttcg gagtcgactt cctcgcgacc
     4921 cggtcggacg acgattcccc ctggtcgctc cacgccctcg agatcaacct ccggatgggc
     4981 gggacgacgc acccctacct cgcgctccag ttcctctcgg gcggccggct cgaccccggc
     5041 tccgggctct tctactcgcc gcgaggcctc ccgaagttct accgcgcgac cgacaacctc
     5101 aagtcggagg cctaccgggg cctcctgccg gaggacctga tcgagatcct gacgatcaac
     5161 ggcctccact attcgcacaa taccgagtcg ggagtgctct tccacctcat cggcgcgctc
     5221 tcgcagtacg gaaagctcgg cgtgaccgcg atcggcaaca gccgcgagga ggtcgagcac
     5281 ctctacgcgc ggacgctggc gatcctggac cgagagactt cgttcgggcg gtaggccccg
     5341 cagacctcgc ggcgcgtctc gccgtcaccg ctcgccacgc ttcgcgcacc gacggcagga
     5401 accaccggcc cgtgcgtcag ccgcccgatg cgttcagggc cgagtcgatg gcccgagaga
     5461 gatccgtgcg gccgagcccg acggcgtgcc gtcgcattcc ctcgacgcgc ggccggagcc
     5521 ccgggtccgc gcggagcccc tccgtaagct ccgccgtcgc cgaggccgcg tccccgcggg
     5581 ccagagagag cacgccgagg ttcacccacg cccgcccgtt cccgggcgcg tcgcggacgg
     5641 tcgcggcgaa cagggccctg tcgtctcgcc agtcgggaat ccggctccag atccgcgacg
     5701 cgcccgcgag cgcgaggagc ccgacggcga gaagggccgc ccgccgaccg ggtcgaggaa
     5761 cccgggcgac gagatcgccg gcgcagaaga cgaccccgac ggccggcagg aagagcagcc
     5821 tttccgtgaa gagaacggcc gtcgggtacg cgacgttcgc gaacgggaga tacgagagaa
     5881 gcgcccaggc gagcccgagc gacgtcatcg gggccctcga ccgcaggcag aacgccgccc
     5941 cggacacacc gagaaggatc gccacccccg cagcgatccg cctcgcgtcc cggaggtcgg
     6001 cgtccgcgat cgcgttcgag ccgtagtcgg cgatcagggt ccgcggccac gtgagacgtt
     6061 ccgccgcgag cggaacgagg gcgacggccc cggagacgcg ctcggcggcc ggcccggacg
     6121 cgagcttgtt ctcgatcgga gcgatccggg cattcggtcc cgtcaggaag ccgccgaggg
     6181 ccgcgagccg gagcgcgaag accccggcgc cgacgagccc ccagcaggcc gccgtcggtc
     6241 cgagacgcga gaggctctcc cgaagccgcc cttcccgaat gcccggcagg acggtcgcgc
     6301 ccaggaggag cggcgccgcg aacgcggtct ccttcgacag gagcccgaga gcgaggacga
     6361 gcgccgaagc cgaggccagg ccccagcggc caggtccggt ccgcttcgcg gaccggagcg
     6421 cgaggaggag cgcgcccgcg acggcggcga acgacagcag ctccgcccgg ccgacgatcg
     6481 cgaggagaac gtcggatcgg gcgggatggg ccgagaagag cacgacgacg acgtctgcca
     6541 cgaccagggg agccccgacc tcgaccacca ggagcccgag cagggccgcg ccgagcgcgc
     6601 cccagagcag gttggagaag cgaagcggca ggggatcgag cccgaaggct cggacgtcga
     6661 gtcccaggga caggcgcgcc gcgggacggt acgcgacccc cgctccgacc tgctcctcgt
     6721 ggaaccaggg cttcagaaag gcctcacgaa tcggcgcctg gcccgtgacc tccggcctcg
     6781 cctcgaccat cgcgtggtcg tcgaagacga agccgttcgg aagcgaaggc aggtaggccg
     6841 cgagatgcac gaggagcgcg agcgcgaagg cccgcgggaa ccgggaggac cgcaccgact
     6901 ccacgggccc atcgtagccc ggctggttcg ggtcctcctc tcgagcgccc gcgcgagcat
     6961 ccgatgccga tgccgggcgc gttcctggcg cgccgctcga cccaagacgt ctcgctcggg
     7021 ctgaagccga ccttcagtcg cgcgccgcca gcgccgccgg caccaggatc gcgtcgttcg
     7081 tcccctggtc gatcgacgtg gcgtaggccc cgacgagccc gcccgccggc gagacccgga
//
LOCUS       DAOTEZ010000213         7128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_7514_length_7128_cov_5.641029, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000213 DAOTEZ010000000
VERSION     DAOTEZ010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7128
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7514_length_7128_cov_5.641029"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="P5164_11875"
     CDS             <1..565
                     /locus_tag="P5164_11875"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRY44631.1"
                     /translation="LRVLQEREVRRLGETRTRPVDLRLVTATHRDLARLCIEGRFRED
                     LLYRIAQLSLTLGPLRERPRDLRALLDAALDGTPLAPEARAALLSWKWPGNVRELLSA
                     VESARALAGPGARIERSHLPPALRTGSGPAVEPGSRYRTAVDEAKRRVVLEALAETGG
                     NRTRAAVLLGLSRQSLLYELKRLAIRD"
     gene            581..2353
                     /locus_tag="P5164_11880"
     CDS             581..2353
                     /locus_tag="P5164_11880"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY44632.1"
                     /translation="MRSLATSLESAAGERARLGAAAAAALALLAVLPFLNGLTAGFTF
                     DDVSIVRDNPRLAGPERVGEIFTTHYFGGPLATGKNFRPVVLLTYAVQRWTTGTDPFP
                     FHLANVLLHAGTTLLLAAWLLALGMPRAPSLAAAALFAVVPIHAEAVTGVVGRAELLV
                     ALLVLLSALLFLRATDGPRLRRAAYAGALGAFLVALFTKEHAVVLPGVVLLGELLRDG
                     AGEPLAARLRRKALPAAGLLAPLAVFVAVRALFVGSGLVAKGSAFFELDNPLVALAAP
                     LRAANGLWLLLRYAGKTLVPLGLSADHSAHALPLALSLSDPRAIAGLLGVVALAALAV
                     LSARRLPLVALGLGLFLGAFFPTSNVPFPIGTIFAERLAYLPSAGLLAAVAGLVAALP
                     RASTGFRRALLTVALVAYAAATVERNEVFRSDESLFADLLVKVPLSARARYNAAWLAW
                     GRGETTVARSNLEKAIALFPRHYDAWALLGLVAAKEGRHVEACAAARESLRIKPDSEI
                     GWRTLAKVEEEAGRLDAAEEALAAGLKRFPRSLPLLRRRGALLLARGRAAEAAETWRT
                     AITVSGGAPEDRLGLAKALRAAPR"
     gene            complement(2391..2954)
                     /locus_tag="P5164_11885"
     CDS             complement(2391..2954)
                     /locus_tag="P5164_11885"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HRY44633.1"
                     /translation="MTFEGRGEESGGGPGERFPISPIPEEGWWSRFRAQFLEMMGRHQ
                     DPEKVAASFAIGVAIAFTPLIGFHFLMAIVLALLLRLNKLDVVLGTLVVNPLTLGPVS
                     AAALPVGRFLLRARQEAVTHMPLGDLLRPDAWAQAGPAMRTIGIHWGIGMFAFSILFG
                     ALTYIVLVRVLRRRRDRAALVPPDFAG"
     gene            complement(2951..3988)
                     /gene="thiL"
                     /locus_tag="P5164_11890"
     CDS             complement(2951..3988)
                     /gene="thiL"
                     /locus_tag="P5164_11890"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HRY44634.1"
                     /translation="MKTLPPETDLLRSFERLFATRRRDVRVGVGDDAAVLAAPSSLAV
                     CTDALVEGEDFTAAADPYLVGRKAMNVNLSDLAAMGATPLHALLTLGLPRETPVAWLD
                     AFLEGFRSAAREHAVAIVGGDLTAARERFASVTVLGRVPDEGPLRRGGGGPGDELWVS
                     GTLGAAAAGLSLVLRGYRRDREGKVTAPRGRRVSPARRDEIARLLRHQLDPRPMVDLG
                     RALAEGGIASAAIDLSDGFARDLHRLCRASGTGAVVETSTLPVDSGLPALPELPTSPR
                     DLALFGGEDYGLLFTVPPERLGAMKKIAARFAVRRVGRLDGTSKVLLAEGGAFTPLAD
                     RGFDHFAGPAG"
     gene            complement(3985..6396)
                     /gene="lon"
                     /locus_tag="P5164_11895"
     CDS             complement(3985..6396)
                     /gene="lon"
                     /locus_tag="P5164_11895"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRY44635.1"
                     /translation="MAEETASVETIQIPDLLPVLPLKDVVLFPYVIVPLSVSRDRSIA
                     AVDAALSEQRILLLLAQKDPSVEEPSPSDLYETGTAAAVMRMLKLPDGRIRLLVQGLS
                     RVKVDGFVQESPSLRAKAVRITEPEPPKPATEEAEALLRAVKEGLEKAVSLGKAISPE
                     VMLVAAGLDDPARLADLAASNLDLKLDEAQTILETLPGLERLKAVLEILRREIRVLTV
                     QQEISGMARGEIDKNQREYFLRQQLKAIQQELGVTDDVAEEVEQYRKKLEGKSLPPEA
                     TEEVEKQLKRLERGNPDSAETATIRTFLDWITGLPWSVTSQDNLDLPRARAILDEDHH
                     GLEKPKERILEFLAVRKLAPDSKGPILCLVGPPGVGKTSLGRSIARALDRKFVQMSLG
                     GVRDEAEIRGHRRTYVGALPGRVIQGINQAATSNPVFILDEVDKIGADFRGDPSSALL
                     EVLDPEQNRAFRDHYLGVPYDLSKVLFITTANVLDTIQPAFLDRMEVIRLSGYTLEEK
                     LAIARRHLVPKQRKENGLSEKGLSFPDDALRTIIEEYTREAGLRNLEREIGSCCRKIA
                     VRIASGRTKPVTMTPALVRELLGVPKFRSDEALARDEVGAATGLAWTAVGGDVLIVEA
                     RALKGKGRLQLTGQLGDVMKESAQAALTLLRSRADELRLPASFFETHDVHVHVPEGAI
                     PKDGPSAGVTMLTAMVSAFTGTPVRRDVAMTGEITLRGEVLPIGGVKEKTLAARRAGV
                     RTILLPAQNERDLAEIPEELRRDLKFVFVERAEQVLENALVRPAAHRSGTRKAARRKT
                     TRRRT"
     gene            complement(6400..6813)
                     /locus_tag="P5164_11900"
     CDS             complement(6400..6813)
                     /locus_tag="P5164_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRY44636.1"
                     /translation="MTKGRPTTRWVYGSGAGDPGAPPPHLPPVDVVEETHGWRLVFEI
                     PGTDPESVRVDVAGRVVVVRGERRPTERAHGTFLCVERSAGPFERAIELPDEPDPDGG
                     SAVYADGLLTLEIPRRPATRRRTIPIRPARGPEKG"
     gene            complement(6810..>7128)
                     /locus_tag="P5164_11905"
     CDS             complement(6810..>7128)
                     /locus_tag="P5164_11905"
                     /inference="COORDINATES: protein motif:HMM:NF015442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HRY44637.1"
                     /translation="DGRREEFDRAKVLRGLRRACEKRPVEEARLEALCDEVEGLFPTR
                     EPRELKTREIGAFLMEKLKEVDQVAFVRFASVYRRFEDAGDFVEEVETLLSDARDLSR
                     RSR"
BASE COUNT          885 a         2616 c         2610 g         1017 t
ORIGIN      
        1 cctgcgggtc ctgcaggaga gggaggtccg aaggctcgga gagacccgga cgcgaccggt
       61 cgacctgcgc ctcgtcacgg cgacgcaccg cgacctcgcc cgcctctgta tcgaggggcg
      121 cttccgggag gacctcctct accggatcgc gcagctctct ctgacgctcg ggccgctccg
      181 cgaaaggccc cgcgacctcc gcgccctcct cgacgcggcg ctcgacggga cgcccctcgc
      241 acccgaggcg cgcgccgccc tcctctcgtg gaagtggccc gggaacgtcc gcgagctcct
      301 ttcggccgtc gagtcggcgc gcgctctcgc cgggccgggc gcgcggatcg agaggagcca
      361 ccttccgcca gcgcttcgca ccgggtccgg ccccgccgtg gagcccggga gccggtaccg
      421 gacggccgtg gacgaggcga aaaggagggt cgtcctcgag gcgctcgccg agacgggggg
      481 aaaccggacg cgcgccgcgg tcctcctcgg gctctcccgc cagtcgctcc tctacgagct
      541 gaagcgtctc gcgatccggg actagacttc gcctctccgg atgcgctctc tcgcgacgtc
      601 cctcgaaagc gccgccggcg agcgcgcgcg cctcggcgcg gcggccgcgg ctgccctcgc
      661 gctcctcgcc gtcctgccgt tcctcaacgg cctgacggcc gggttcacgt tcgacgacgt
      721 ctccatcgtg cgggacaacc cccgcctcgc cgggccggag agggtcggcg agatcttcac
      781 gacgcactac ttcggcggtc cgctcgcgac cgggaagaac ttccggcccg tcgtcctcct
      841 cacctacgcc gtccagcgct ggaccaccgg gaccgacccg ttcccgttcc acctggcgaa
      901 cgtcctcctc cacgcgggga cgacgctcct cctcgccgcc tggctcctgg ccctcgggat
      961 gccgcgcgcg ccgtcgctcg ccgcggcggc tctcttcgcg gtcgtgccga tccacgccga
     1021 ggccgtaacc ggggtcgtcg ggcgcgccga gctcctcgtc gcgctcctcg tcctcctctc
     1081 cgcgctcctc ttcctccggg ccacggacgg cccgcgcctg cggcgtgccg cgtacgccgg
     1141 cgcgctcggg gccttcctcg tcgccctctt cacgaaggag cacgccgtcg tcctgccggg
     1201 cgtcgtcctc ctcggcgagc tcctccggga cggggcgggc gagccgctcg ccgcgaggct
     1261 ccggcgcaag gccctcccgg cagccgggct cctcgccccg ctggccgtct tcgtcgccgt
     1321 ccgggcgctc ttcgtcggga gcggcctcgt ggcgaagggg agcgcgttct tcgagctcga
     1381 caacccgctc gtcgccctcg cggcgccgct tcgcgccgcg aacggcctct ggctcctcct
     1441 gcgctatgcg gggaagacgc tcgtcccgct cggcctctcc gcagaccact cggcgcacgc
     1501 gctcccgctg gcgctctcgc tctcggaccc gagggcgatc gccgggctgc tcggcgtcgt
     1561 cgccctcgcc gcgctcgccg tcctctcggc tcgaaggctc ccgctcgtcg cgctcgggct
     1621 ggggctcttc ctcggcgcgt tcttcccgac ctcgaacgtc ccgttcccga tcgggacgat
     1681 cttcgccgag cgcctcgcct acctcccgtc ggcggggctc ctcgcggcgg tcgcggggct
     1741 cgtggccgcg ctgccccgcg cttccaccgg tttccggcgg gcgctgctga ccgtcgccct
     1801 cgtggcgtac gccgcggcga cggtcgagag gaacgaggtc ttccggagcg acgagagcct
     1861 cttcgccgac ctgctcgtca aggtcccgct ctcggcccgg gcccgctaca acgcggcctg
     1921 gctcgcctgg ggccggggcg agacgaccgt cgcccgctcg aacctcgaga aggcgattgc
     1981 cctcttcccg cgccactacg acgcctgggc cctcctcggc ctcgtcgcgg cgaaggaggg
     2041 gcgccacgtg gaggcgtgcg ccgcggcgcg ggagtccctc cggatcaagc ccgactccga
     2101 gatcgggtgg cggacgctcg cgaaggtgga ggaggaggcc gggcgtctcg acgcggccga
     2161 ggaggcgctc gccgccggcc tgaaacggtt cccgcgctcg ctccctctcc tccggcggcg
     2221 cggcgcgctc ctcctcgcgc ggggccgcgc ggccgaggcc gccgagacct ggcggacggc
     2281 gatcaccgtc tccggcggcg ccccggagga ccgcctcggc ctcgcgaagg cgcttcgcgc
     2341 cgctccccgc tgaggagcac gcggcgcggc ggtccgttcc tccgggaggg tcagcccgcg
     2401 aagtcgggcg gcacgagcgc ggcgcggtcg cgccgtcggc gaaggacccg gacgaggacg
     2461 atgtacgtga gggcgccgaa gaggatcgag aaagcgaaca tcccgatccc ccagtggatc
     2521 ccgatcgtcc gcattgccgg gccggcctgc gcccaggcgt cgggccggag caggtcgccc
     2581 agcggcatgt gggtgacggc ctcctggcgc gcccggagga ggaaccggcc caccggaagc
     2641 gcggccgcgc tcacgggccc gagagtcagg gggttgacga cgagagtccc gaggacgacg
     2701 tccagcttgt tgagccggag gaggagggcg aggacgatcg ccatcaggaa gtggaatccg
     2761 atgagcggcg tgaacgcgat cgcgacgccg atcgcgaacg acgcggcgac cttctccgga
     2821 tcctggtggc gtcccatcat ctcgaggaac tgcgccctga agcgcgacca ccacccctcc
     2881 tcgggaatcg gcgagatcgg gaagcgctcc ccggggcctc cgccgctctc ctcgccccgt
     2941 ccttcgaacg tcatccggcc ggcccggcga agtggtcgaa gccgcgatcg gcgagaggcg
     3001 tgaaggcgcc gccctcggcg aggaggacct tcgacgtgcc gtcgagccgg ccgacccggc
     3061 ggaccgcgaa gcgcgccgcg atcttcttca tcgcgccgag gcgctccgga gggaccgtga
     3121 agaggagccc gtagtcctcg ccgccgaaga gcgcgaggtc gcgcggggag gtcgggagct
     3181 cgggcagcgc cgggaggccg gaatcgacgg ggagcgtcga ggtctcaacg acggcgcccg
     3241 tcccggaggc gcggcagagg cggtggaggt cgcgagcgaa cccgtcggag aggtcgatcg
     3301 ccgccgacgc gatccccccc tcggcgagcg cccggccgag atccaccatc gggcgcgggt
     3361 cgagctggtg ccggaggagc cgggcgatct cgtcgcgacg ggccggtgac acgcggcgcc
     3421 cgcgcggagc ggtcaccttt ccctcgcggt cgcggcggta tccgcgcagg acgagcgaca
     3481 gtcccgccgc ggcggcgccg agcgtcccgg agacccagag ctcgtcgccc gggccgccgc
     3541 ccccgcggcg cagcggccct tcatcgggga cgcggccgag gacggtgacc gaagcgaagc
     3601 gctcccgcgc ggcggtcagg tcgccgccga cgatcgccac ggcgtgctcc cgcgcggccg
     3661 aacggaagcc ctcgaggaaa gcgtccagcc aggcgaccgg cgtctcgcga gggaggccga
     3721 gcgtgaggag cgcgtggagg ggcgtcgccc ccatcgcggc gaggtccgag aggttcacgt
     3781 tcatcgcctt ccgcccgacg agatacgggt cggcggcggc ggtgaagtcc tccccctcca
     3841 cgagggcgtc ggtgcagacg gcgagggagg agggagcggc gaggacggcg gcgtcgtcgc
     3901 cgacgccgac cctcacgtcg cgccggcgcg tcgcgaagag gcgttcgaag ctgcgaagga
     3961 ggtcggtctc gggggggagc gtcttcacgt tcgcctcctg gtcgtcttcc ttcgcgccgc
     4021 cttgcgcgtg cccgagcggt gcgcggcggg gcggacgagg gcgttttcga ggacctgctc
     4081 ggcgcgctcg acgaagacga acttgaggtc gcgccgcagc tcctctggga tctccgcgag
     4141 gtcgcgctcg ttctgcgcgg ggagaaggat cgtccggacg ccggcgcgcc gggcggcgag
     4201 cgtcttctcc ttcacgccgc cgatcgggag cacctcgccc cggagcgtga tctcgccggt
     4261 catcgcgacg tcgcggcgga cgggcgtccc ggtgaaggcg gagaccatcg ccgtcagcat
     4321 cgtgacgccg gccgacgggc cgtccttcgg gatcgccccc tcggggacgt ggacgtgaac
     4381 gtcgtgcgtc tcgaagaagg aggccgggag ccgcagctcg tccgcgcggg agcgcaggag
     4441 cgtgagcgcg gcctgcgccg actccttcat cacgtctccg agctggcccg tgagctggag
     4501 gcgccccttc cccttcagag cgcgcgcctc cacgatgagg acgtcgccgc cgacggcggt
     4561 ccaggcgagg cccgtcgctg cgccgacctc gtcccgtgcg agcgcctcgt cgcttcggaa
     4621 cttcggcacg ccgagcagct cgcggacgag cgccggggtc atcgtcaccg gcttcgtcct
     4681 cccggaggcg atccggaccg cgatcttccg gcagcagctc ccgatctccc gctccaggtt
     4741 ccggaggccc gcctcgcgcg tgtactcctc gatgatggtc cggagggcgt cgtcggggaa
     4801 cgagagcccc ttctcggaga ggccgttctc cttccgctgc ttcggcacga ggtggcggcg
     4861 cgcgatcgcg agcttctcct cgagcgtgta gcccgagagc cggatcacct ccatccggtc
     4921 gaggaaggcc ggctggatcg tgtcgaggac gttcgcggtc gtgatgaaga ggaccttcga
     4981 gaggtcgtag gggactccga ggtagtggtc ccggaacgcc cggttctgct cggggtcgag
     5041 gacctcgagg agggccgagg acgggtcgcc gcggaagtcg gccccgatct tgtcgacctc
     5101 gtcgaggatg aagacggggt tcgacgtggc ggcctggttg atcccctgga tgacgcggcc
     5161 cggcagggcg ccgacgtacg tgcggcggtg gccgcggatc tcggcctcgt cccgcacgcc
     5221 cccgagcgac atctggacga acttccggtc gagggcccgc gcgatcgacc ggccgagcga
     5281 ggtcttcccg acgccgggcg ggccgacgag gcagaggatc ggccccttgc tgtcgggggc
     5341 gagcttccgg acggccagga attcgaggat ccgctccttc ggcttctcga ggccgtggtg
     5401 gtcctcgtcg aggatcgccc gggccctcgg caggtccagg ttgtcctgcg aggtgacgct
     5461 ccaggggagc ccggtgatcc agtcgagaaa cgtccggatc gtcgcggtct cggccgagtc
     5521 ggggttgccg cgctcgagcc gcttcagctg tttctcgacc tcctcggtcg cctcgggcgg
     5581 aaggctcttc ccctcgagct tcttccggta ctgctcgacc tcctcggcga cgtcgtcggt
     5641 gacgccgagc tcctgctgga tcgccttcag ctgctgccgg aggaagtact cccgctggtt
     5701 cttgtcgatc tcgccgcggg ccatccccga gatctcctgc tggacggtca ggacgcggat
     5761 ctcgcggcgg aggatctcga gcaccgcctt gaggcgctcg aggccgggga gcgtctcgag
     5821 gatcgtctgc gcctcgtcca gcttcaggtc gaggttcgag gcggcgaggt cggccagccg
     5881 cgccgggtcg tcgagtcccg ccgcgacgag catcacctcg ggggagatcg ccttccccag
     5941 agagacggcc ttctccagcc cctccttgac ggcccgaagg agcgcctcgg cctcctccgt
     6001 cgccggcttc ggcggctcgg gctcggtgat ccggaccgcc ttggcgcgca gggacgggct
     6061 ctcctggacg aaaccgtcga ccttcacccg ggagagtccc tggacgagaa gccggatgcg
     6121 gccatcgggg agcttcagca tccgcatgac ggcggctgcg gtcccggtct cgtagaggtc
     6181 ggagggcgag ggctcctcga ccgacgggtc cttctgggcg aggaggagga ggatccgctg
     6241 ctcggagagc gccgcgtcga ctgcggcgat cgatcggtcg cgggagacgg agagcgggac
     6301 gatcacgtac gggaagagga cgacgtcctt cagcggcagg accggaagga ggtccgggat
     6361 ctggatcgtc tcgacgctcg cggtctcttc ggccatcggc tagcccttct ccgggccccg
     6421 cgccgggcgg atcgggatcg tccgccgccg ggtggcgggg cggcggggga tctcgagcgt
     6481 gaggagcccg tcggcgtaga cggccgaccc gccgtccggg tcgggctcgt cggggagctc
     6541 gatcgcgcgc tcgaacgggc ccgcggagcg ctccacgcag aggaacgtgc cgtgcgcccg
     6601 ctccgtaggc cgtcgctcgc cccgcaccac gacgacgcgc cccgccacgt cgacccggac
     6661 cgactccggg tcggtcccgg ggatctcgaa gacgagccgc cagccgtggg tctcctcgac
     6721 gacgtcgacc ggggggaggt gaggcggcgg cgcgccgggg tcgcccgcgc cggagccgta
     6781 gacccagcgc gtcgtcggtc gtcctttcgt catcggctcc tccgtgagag gtcgcgggcg
     6841 tcggagagga gggtctcgac ctcctcgacg aagtcgccgg cgtcctcgaa ccgccggtag
     6901 acggaggcga agcggacgaa ggcgacctgg tcgacctcct tcagcttctc catcaggaag
     6961 gccccgatct cgcgcgtctt cagctcgcgc ggctcgcgcg tcggaaagag cccttcgacc
     7021 tcgtcgcaga gagcttcgag gcgggcctcc tcgacggggc gtttctcgca ggcgcgccgg
     7081 aggccgcgca gcaccttcgc gcggtcgaac tcctcgcggc ggccgtcg
//
LOCUS       DAOTEZ010000214         7107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_7545_length_7107_cov_5.807998, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000214 DAOTEZ010000000
VERSION     DAOTEZ010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7107
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7545_length_7107_cov_5.807998"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="P5164_11910"
     CDS             <1..256
                     /locus_tag="P5164_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44638.1"
                     /translation="RFRAVRYRSEVTEGRTDFGAELDELEPVRVKVRGWGIEAVASAF
                     SDERFERFAAKDGIVDAEAAPLSLEELFLAVAGEGKDLAR"
     gene            253..2199
                     /locus_tag="P5164_11915"
     CDS             253..2199
                     /locus_tag="P5164_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44639.1"
                     /translation="MKRFAAVALREIAERRFVLVAAAAAAVLPFLVPLLPSVPGSDAA
                     TARAVTALVLACTFGAGGAVLVGASVVGRELAEKRLSFHFARPLPAAVVWGGKLGGGL
                     ALVLLAELVVYLPASIASGRFPGLVDRFDAPAMAALLLLAVPLFLIAWVASVAVRSRS
                     PWLVVDFVLLAASAAAVFLLGRRLLRYGHVPGPSAALAVVGVLLAALLAGTLAQVAAG
                     RTDARRGHGAQSLALWSVLLAGLAVGASWVSRTVDPGVARLQGAMAEPAGRGGDWAVV
                     AGSTPGAGSGTSAYLLDLAGGRSVLLRPGWMTTVSTDGSRAAHVAAGGVRGAKVELEA
                     IDLRTGDAVVLPLSEWPDGIDLTPDGRRLAVVAGGLCQVLDLPSFRLLASARVPAERW
                     AFAPAFLGSDVVRLLPKHLRSGENAARSPGPVPDPDSWEMRVASRRMVKLARLQVSAL
                     PARPPDPKEPGREPRYAFLPGPDRSRVVLFGLGSTTGARLLDATTGAVLASVDAAAAP
                     GPRGFFLSDGRVVLSEPDPDGLTLHLLSPDGARLASIRMPAPTHGLRFGSEPGRDLLQ
                     VGLARDEARTTWGWYLVDLATGTVTPIEGVPPQRSWWSGALSYPAPGSPVTRLSNDAD
                     GRLVLRDPATGEARPLTRGGPPGK"
     gene            2210..3178
                     /locus_tag="P5164_11920"
     CDS             2210..3178
                     /locus_tag="P5164_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HRY44640.1"
                     /translation="MTTPARREARRVLEIEAAAIRSLAESLDGGFDTAVALLHGTTGR
                     VVVTGIGKSGIVGQKIAATLASTGTPAFFLHPAEAIHGDLGMILAGDVVLALSHSGET
                     EEVVALLPHVRRRGAKLVALTGRRASTLGREADAVVEAAIREEACPLNLAPTASTAAQ
                     LAMGDALAMALSVEKGFRPEDFAALHPGGKLGKRFLRVSDLMHAEGDVPLVPLEAPMK
                     DVVYEMSKKRLGITGVVDADGRLAGVVSDGDLRRLLEREGERAWSADAAFAMNRTPKT
                     IAAEAFATEALRLMEEKKITSLFIVDGDGRPGGIVHLHDLWGVESI"
     gene            3211..3816
                     /locus_tag="P5164_11925"
     CDS             3211..3816
                     /locus_tag="P5164_11925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HRY44641.1"
                     /translation="MPAAPIVPDDLRTKLLRVRALVLDVDGVLTDGTLTFDEDGRELK
                     SFDVKDGLGLVLLRDLGYRIGLISSRVSKVVALRCRDLKIPEECVMQGELDKASAFDR
                     LLGKWGFEAFEAVAVGDDLPDLGMMRRAGVGACPSDAVSIVKDAAALHLTKPGGGGAV
                     RELCDLILEIRRGARADKPQEVKPPQASSDGSVLPFPGSRG"
     gene            complement(3860..4615)
                     /gene="tatC"
                     /locus_tag="P5164_11930"
     CDS             complement(3860..4615)
                     /gene="tatC"
                     /locus_tag="P5164_11930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRY44642.1"
                     /translation="MTDAPADDLTRMTFLEHLEELRKRLLWSVIAVAAGFFVAWWKAQ
                     DLFRIAQKPILEVLPAGTKLAYTNLTEPFMLYLNIALIAGIFLASPVVIYQVWLFVAP
                     GLYRHEKRWVLPFVFFAAISFCGGGWFGYAVAFPMVAQFLVTMGADFTPVLKIDDYLS
                     ILSKILLGMGLVFQTPIVIVFFVRIGVVTEKWLLQKFRYAVLVIFLIAALITPTPDIP
                     TQCAFALPMIALYLLGILLAWIVGKRTKTEPAV"
     gene            complement(4612..4890)
                     /locus_tag="P5164_11935"
     CDS             complement(4612..4890)
                     /locus_tag="P5164_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRY44643.1"
                     /translation="MGNLGMPELIFIFALALLILGPKKLPELGKQLGKGLGEFKRASN
                     DLKRSIEEEVEKVAHDEAPKRDAAPKAVPAEGTVASAAEERPDPIRPA"
     gene            5039..6655
                     /locus_tag="P5164_11940"
     CDS             5039..6655
                     /locus_tag="P5164_11940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRY44644.1"
                     /translation="MTPKPPRSRALKLLAAPLVLAATTLLGGAFGPRLLAVSDATNPV
                     LREYSDLLATARAWYADDLGSERLVYSSISGMLDTLDPHSSFLEPEEYGAMQEKQRGS
                     FYGLGIIISKRNGRVTVITPVEGSPAQRLGIRAGDIIDLVEGQPIDDLPIDAVVKKLK
                     GPKGTKVSITIVRPGLGEPLQMTVTRAEIPTNSVSFAFMVEPEVGYIRLKDFTHTSAP
                     EVAEAWEKLEKQGMKKLVFDLRANPGGLLDQAIAVSDFFLRKGEKVVVTRGRAAGSEQ
                     TFTAPGRNSRARIPVVVLINKGSASAAEIVAGAVQDQDRGIVAGQTSWGKGLVQSVYT
                     LSEGAGLALTTAKYYTPSGRCIQRDYREFIEYVAPDEEEDGDPLLAEDGVKRESFRTA
                     SGRVVYGGGGITPDVVVKAERLSRWMATLYARGVFFEWAVQYRAKNPEVPRDFRVTDA
                     VRDDFFAFLDARPNAPDAPARASWAAEKDKAVLDRALGEELVGAVHGPEAAYRLSIDG
                     DVQLKKAITLFPEAEKLAGIGSSPEAIAKR"
     gene            6700..>7107
                     /locus_tag="P5164_11945"
     CDS             6700..>7107
                     /locus_tag="P5164_11945"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HRY44645.1"
                     /translation="MKLGIVGRPKTGKTTLFNLLTRSHQSVDKFGAVRETHVGTAHVP
                     DPRLDRLSAMFEPKKHTPATVDFVDVPGIAKGEQEKIDLAALKNVDALVHVVRAFEDA
                     DIPHEGGLDPARDAKALDEELVLSDYVLLEKRRE"
BASE COUNT          967 a         2668 c         2458 g         1014 t
ORIGIN      
        1 ccgcttccgc gccgtccgct accggagcga ggtgacggaa gggcggaccg acttcggcgc
       61 cgagctcgac gagctggagc ccgtccgcgt gaaggtccgc ggctggggga tcgaggccgt
      121 cgcctcggcc ttctccgacg agcgcttcga gcgcttcgcc gcgaaggacg ggatcgtcga
      181 cgccgaggcc gcccccctct ccctcgagga gctcttcctc gccgtcgccg gcgagggaaa
      241 ggacctcgcc cgatgaagcg cttcgccgcc gtcgccctcc gcgagatcgc ggagcggcgc
      301 ttcgtcctcg tcgccgccgc cgccgcggcc gtcctcccgt tcctcgtgcc gctcctcccg
      361 tcggtgcccg ggagcgacgc cgcgacggcc cgcgccgtca cggcgctcgt cctcgcctgc
      421 acgttcggcg ccggcggggc cgtcctcgtc ggcgcctccg tcgtcggccg cgagctcgcg
      481 gagaagcgtc tctcgttcca cttcgcccgg ccgctccccg ccgccgtcgt ctggggcggg
      541 aagctcggcg gcggcctcgc gctcgtcctc ctcgcggagc tcgtcgtcta cctgccggcc
      601 tccatcgcga gcggtcgctt cccggggctc gtcgaccgct tcgacgcccc cgcgatggcg
      661 gcgctcctcc tcctcgcggt cccgctcttc ctcatcgcct gggtcgcctc cgtggcggtt
      721 cgctcccgct cgccctggct cgtcgtcgac ttcgtcctcc tcgccgcgtc ggcggccgcg
      781 gtcttcctcc tcggccgcag actcctccgc tacggccacg ttcccgggcc atccgccgcc
      841 ctcgccgttg tcggggtcct cctcgcggcg ctcctcgccg ggactcttgc gcaggtcgcc
      901 gcggggcgca cggacgcgcg ccgcggacac ggcgcgcagt cgctcgcgct ctggagcgtc
      961 ctcctcgccg gcctcgcggt gggcgcctcc tgggtgagcc ggacggtcga cccgggggtg
     1021 gcccgcctcc agggggcgat ggccgagccc gcgggaagag gcggcgactg ggccgtcgtg
     1081 gccggctcga cgcccggggc cggatcggga acctccgcgt acctcctcga cctggcgggc
     1141 ggccgcagcg tccttctccg gccgggctgg atgacgacgg tctcgaccga cggctcccgc
     1201 gccgcccacg tcgccgcggg cggcgtccgc ggcgcgaagg tcgagctcga ggcaatcgac
     1261 ctccggacgg gggacgccgt cgtcctccct ctgtcggagt ggcccgacgg tatcgacctc
     1321 acccccgacg gccgccggct cgccgttgtc gccggcggac tctgccaggt cctggacctc
     1381 ccctcgttcc gcctcctcgc gtccgcccgc gtgccggcgg agcgttgggc cttcgcgccg
     1441 gctttcctcg gatccgacgt cgtccgtctc ctcccgaagc acctccgttc cggggagaac
     1501 gccgcccgaa gccccggacc ggtcccagat cccgacagct gggagatgcg cgtcgcgtcg
     1561 cgccgcatgg tgaagctcgc gcggctccag gtgtcggccc ttccggcccg accgcccgac
     1621 ccgaaggagc ccggccgcga gccgcgatac gcgttcctgc ccggaccgga ccgttcccgc
     1681 gtcgtcctct tcgggctcgg ctcgacgacg ggcgcgcgcc tcctcgacgc gacgacgggc
     1741 gcggttctcg cgtccgtcga cgccgcggcc gcgccggggc cgaggggctt cttcctctcg
     1801 gacggccgag tcgtcctctc cgagcccgat cccgacggcc tgacgctcca tctgctttcg
     1861 cccgacggag cccgcctcgc ctcgatccgg atgccggctc ccacgcacgg cctccggttc
     1921 ggcagcgagc cgggccggga tctcctccag gtcggtctgg ctcgggacga ggcccggacg
     1981 acgtggggct ggtacctcgt cgacctcgcc acggggacgg tgacgccgat cgagggcgtg
     2041 ccgccgcagc gctcctggtg gtccggcgcg ctctcctacc ccgcgccggg ctctcccgtc
     2101 acccgcctct caaacgacgc cgacggccgg ctcgtcctcc gcgacccggc aaccggcgag
     2161 gcgaggcccc tcacgcgcgg aggaccgccc ggcaagtaag atcgtccccg tgaccacccc
     2221 cgcacgccgc gaagcccgcc gcgtcctcga gatcgaggcc gcggcgatcc gctccctcgc
     2281 cgaatccctc gacggcggct tcgacacggc cgtcgccctc ctccacggga cgacgggccg
     2341 cgtcgtcgtg accggcatcg gaaagagcgg gatcgtgggc cagaagatcg ccgcgacgct
     2401 cgcgtcgacg gggacgccgg cgttcttcct ccaccccgcc gaggcgatcc acggcgacct
     2461 cgggatgatc ctcgccgggg acgtcgtcct cgccctctcg cactccggcg agacggagga
     2521 ggtcgtggcg ctcctgccgc acgtgaggcg gcgcggcgcg aagctcgtcg ccctcaccgg
     2581 gcgccgcgcc tcgacgctcg gcagggaagc cgacgccgtc gtcgaggcgg cgatccgcga
     2641 ggaggcctgc cccctgaacc tcgcgccgac ggcctccacg gcggcccagc tcgcgatggg
     2701 cgacgccctc gcgatggcgc tgtcggtcga gaagggcttc cggccggagg acttcgccgc
     2761 gctccacccg gggggcaagc tcggcaagcg ctttctccgg gtctccgacc tgatgcacgc
     2821 ggagggggac gtgccgctcg tcccgctcga agctccgatg aaggacgtcg tctacgagat
     2881 gtcgaagaag cggctcggga tcaccggcgt cgtcgacgcc gacgggcgcc tcgccggcgt
     2941 cgtctccgac ggagacctcc ggcgccttct cgagcgcgag ggggagaggg cctggagcgc
     3001 cgacgcggcc ttcgccatga accgcacgcc gaagacgatc gcggcggagg cgttcgccac
     3061 ggaggcgctc cgcctgatgg aggagaagaa gatcacgtcg ctcttcatcg tggacggcga
     3121 cgggcgcccc ggggggatcg tccacctcca cgacctctgg ggggtcgagt cgatttgagc
     3181 ccggggccgg ctggcggata atccaccgtc atgcccgcag caccgatcgt ccccgacgac
     3241 ctccggacga agctcctccg ggtccgcgcc ctcgtcctcg acgtggacgg agtgctcacc
     3301 gacgggacac tcactttcga cgaggacgga cgggagctga agtcgttcga cgtgaaggac
     3361 gggctcggcc tcgtcctgct ccgcgacctc ggataccgga tcggcctgat ctcgagccgc
     3421 gtctcgaagg tcgtcgccct gcgctgccgc gacctgaaga tccccgagga gtgcgtgatg
     3481 cagggcgagc tcgacaaggc ttccgccttc gaccgcctcc tcgggaaatg gggattcgag
     3541 gcgttcgagg cggtggccgt cggagacgac ctccccgacc tcgggatgat gcggcgcgcc
     3601 ggggtcggcg cctgcccgtc cgacgccgtc tcgatcgtga aggacgcggc ggccctccac
     3661 ctgacgaagc cgggcggcgg cggcgccgtg cgggagctct gcgacctgat cctcgagatc
     3721 cggcgcgggg cccgcgccga caagccgcag gaggtcaagc cgccccaggc gtcgtcggac
     3781 ggctccgtcc tccccttccc cggctcgcgc gggtaggccg caggcgccgt tcgccggcgg
     3841 cggccggaag ccgccggctt cagaccgccg gctcggtctt cgtccgcttc ccgacgatcc
     3901 aggcgaggag gatcccgagg aggtagagcg cgatcatcgg gagagcgaag gcgcactgcg
     3961 tcgggatgtc cggcgtcggc gtgatgagcg ccgcgatcag gaagatgacg aggacggcgt
     4021 agcggaactt ctgcaggagc cacttctccg tcacgacgcc gatccggacg aagaagacga
     4081 tgacgatcgg cgtctggaag acgagcccca tcccgaggag gatcttcgag aggatcgaga
     4141 ggtagtcgtc gatcttcagg accggggtga agtcggcccc catcgtcacg aggaactgcg
     4201 ccaccatcgg gaaggcgacc gcgtacccga accaccctcc gccgcagaag gagatcgccg
     4261 cgaagaagac gaacggcagg acccagcgct tctcgtgacg atagagcccg ggcgcgacga
     4321 aaagccacac ctgatagatc accacggggc tcgcgaggaa gatccccgcg atcagggcga
     4381 tgttcaggta gagcatgaag ggctctgtca ggttcgtgta ggcgagcttc gtcccggccg
     4441 gcaggacctc gaggatcggc ttctgcgcga tccggaagag gtcctgggcc ttccaccacg
     4501 ccacgaagaa gccggccgcc acggcgatca ccgaccagag gaggcgtttg cggagctcct
     4561 cgaggtgctc gaggaacgtc atccgcgtga ggtcgtcggc gggtgcgtcc gtcacgcggg
     4621 ccggatcggg tcggggcgct cttcggcggc gctcgcgacg gtcccctcgg cggggacggc
     4681 tttcggggcg gcgtccctct tcggcgcctc gtcgtgcgcc accttctcca cctcctcctc
     4741 gatcgaccgc ttgaggtcgt tcgaggccct cttgaactcg ccgagaccct tcccgagctg
     4801 cttgccgagc tcggggagct tcttcggtcc gaggatcagg agggccagag cgaagatgaa
     4861 gatcagctcc ggcattccga ggttgcccat ggccgcggaa tcgtctgctc cgggcccgcc
     4921 ggagtcaagg tcgacgtccc gggcctcacg ggccgggctc cgagccttgg cgagagcggg
     4981 gctcggccct ctcccgcctc cggcacgccg catgctaccg tccgccgcgg aggcctccgt
     5041 gacgccgaaa ccgccccgct cccgcgccct gaagctgctc gccgccccgc tcgtcctcgc
     5101 cgcgacgacg ctcctcggcg gagccttcgg accccgcctc ctcgcggtct ccgacgcgac
     5161 gaacccggtc ctcagggagt actccgacct cctcgcgacc gcgcgggcgt ggtacgccga
     5221 cgacctcggc tcggagcggc tcgtctactc ctccatctcc gggatgctcg acaccctcga
     5281 cccgcactcc agcttcctcg agccggaaga gtacggggcg atgcaggaga agcagcgcgg
     5341 ctcgttctac ggcctcggga tcatcatctc gaagcggaac ggccgcgtca cggtgatcac
     5401 gcccgtcgag ggctcccccg cccagcggct cggcatccgg gccggcgaca tcatcgacct
     5461 cgtcgagggg cagccgatcg acgacctccc gatcgacgcc gtcgtgaaga agctgaaggg
     5521 gccgaagggg acgaaggtga gcatcacgat cgtccggccc ggcctcggcg agccgctcca
     5581 gatgacggtc acgcgcgccg agatcccgac gaactccgtc tcgttcgcct tcatggtgga
     5641 gccggaggtc gggtacatcc gcctgaagga cttcacgcac acctccgccc ccgaggtcgc
     5701 cgaggcctgg gagaagctcg agaagcaggg gatgaagaag ctcgtcttcg acctgcgcgc
     5761 gaacccgggc ggcctcctcg accaggcgat cgcggtctcc gacttcttcc tccgcaaggg
     5821 ggagaaggtc gtcgtcacgc gcggccgggc cgcgggctcc gagcagacct tcacggcacc
     5881 gggccggaac tcccgcgccc ggatccccgt cgtcgtcctc atcaacaagg gctccgcgtc
     5941 ggcggccgag atcgtcgccg gcgccgtcca ggaccaggac cgcggcatcg tcgccggcca
     6001 gacgagctgg ggtaagggcc tcgtgcagtc ggtctacacc ctctccgagg gggccggcct
     6061 cgcgctgacg acggccaagt actacacgcc gtcggggcgc tgcatccagc gcgactaccg
     6121 ggagttcatc gagtacgtcg cgccggacga ggaggaggac ggagacccgc tcctcgccga
     6181 ggacggcgtg aagagagagt ccttccgcac ggcgagcggc cgggtcgtct acggcggagg
     6241 cggcatcacg cccgacgtcg tcgtgaaggc cgagaggctc tcgcgctgga tggcgaccct
     6301 ctacgcgcgg ggcgtcttct tcgagtgggc cgtgcagtac cgggcgaaga accccgaggt
     6361 cccccgcgat ttccgcgtga ccgacgcggt ccgcgacgac ttcttcgcct tcctggacgc
     6421 ccgtccgaac gccccggacg cgccggcacg ggcctcctgg gccgcggaga aggacaaggc
     6481 cgtcctcgat cgcgccctgg gcgaggagct cgtcggagcg gtgcacgggc ccgaagcggc
     6541 ctaccgcctc tcgatcgacg gggacgtcca gctgaagaag gcgatcacgc tcttccccga
     6601 ggccgagaag ctggccggga tcggctcgtc tcccgaggcg atcgcgaaga ggtagccgcc
     6661 cgaagcggcc gcgggacgtt ctgctacgct cccgcggcca tgaagctcgg gatcgtcggc
     6721 cggccgaaga ccggaaagac cactctcttc aacctcctga cccgttccca ccagtcggtc
     6781 gacaagttcg gcgccgtccg cgagacgcac gtcgggacgg cgcacgtccc cgatccgcgg
     6841 ctcgaccggc tctcggcgat gttcgagccg aagaagcaca cgccggcgac cgtcgacttc
     6901 gtcgacgtcc ccgggatcgc gaagggcgag caggagaaga tcgacctggc ggcgctgaag
     6961 aacgtcgatg cgctcgtcca cgtcgtgcgc gcgttcgagg acgccgacat cccccacgag
     7021 ggaggcctcg accccgcgcg cgacgcgaag gcgctcgacg aggagctggt cctctcggac
     7081 tacgtcctcc tcgagaagcg gcgcgag
//
LOCUS       DAOTEZ010000215         7055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_7613_length_7055_cov_6.446714, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000215 DAOTEZ010000000
VERSION     DAOTEZ010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7055
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7613_length_7055_cov_6.446714"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="P5164_11950"
     CDS             complement(<1..810)
                     /locus_tag="P5164_11950"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRY44646.1"
                     /translation="MALAGLALAAGSEAAAAKTAREGAPSVLAEAARAPSASPAPCPF
                     RDPEDGGCFSSADEADLAWIARASAAAPRAPKVALPPGLPAVLAVARTQAEEHLPLEA
                     DPDGEEPYEEGSWAAGASPAAPLPALSEEQRSFWRDLRSTLKGLVVRKKDSKPASIPE
                     EELAALFATGFPLPIEAFPKEKLRDTFDASRGRHKRHHAIDLGAPKGTPIVAVSDGKI
                     ERLGRDRRGGIVIYQRDASGRFVYYYAHLSRYAPGLKVGDTVKRGQQIGEVG"
     gene            complement(834..1394)
                     /gene="efp"
                     /locus_tag="P5164_11955"
     CDS             complement(834..1394)
                     /gene="efp"
                     /locus_tag="P5164_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRY44647.1"
                     /translation="MISATELRAGMIVIHNGELHRAHSVVHKTPGNLRGFVQAKLRSL
                     KSGAMNEHRFRSEDKIEKATLDTHEMQYLYSDGEGHHFMNQETYDQVRLSDEIVGDAM
                     KYLLPETVIDVDFYDGNPISVELPTTVNLKVVETEPGMKGATASASYKPAKLETGLIV
                     QIPQFVEAGTVIKIDTRDDSYLERAG"
     gene            complement(1551..3056)
                     /locus_tag="P5164_11960"
     CDS             complement(1551..3056)
                     /locus_tag="P5164_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44648.1"
                     /translation="MKTTKTTLLLALVLALAATASPAAAQADFSKFVAIGASVDAGFL
                     DNCWVRYGQVDSWNAILARQAGAPAFEQPLLDTPGTGCMYLISLAPQFGTRPAQIKPL
                     NLNLPRPYDNLSIPGYTLQAALTKKGPSSASDVAGLVLRTLNATQVEQAAALKPTFVS
                     IGLFGNEILGPATSGTAVDGVTVMPAAMYAASYQAVVNTLKAAQGGTGKGVAQLIPDI
                     TTIPFTSTIPPFLTSGGQVVMGPNGQPLTFLSSRGGDVTGGGAGVAPIPATSLVNITA
                     SAFLATGYGIPCAVLDAGGAPANDPRRTNCNKPLPDNANAQLGIPGVVLYPEEVALLK
                     TRGAEFNAQIRSIATAAGYKVFDTTAFFTDLRTHGREYAGITVNAAFLSGGLISYDGV
                     HPTSLGYAVWADEMVRFINANYGTTIPRVDISGYLFSGNSSQGGYPVNLGANLTTEEA
                     VAWGAAVYTPEVMRTLAETFPVSARTAVAAPAAEEAPVPAEPRGRSARIDD"
     assembly_gap    3167..3266
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3267..4160)
                     /locus_tag="P5164_11965"
     CDS             complement(<3267..4160)
                     /locus_tag="P5164_11965"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HRY44649.1"
                     /translation="MRGSDRYRAGGLALLLAISLVAAGPASAAGFSIFEAGSRATAMG
                     GAFVAHADDLSAMFYNPAGLAQGVKKGELRAMAGVTLIVPKAELAQGYDPYPGSDYSV
                     KMADQFFFPPNFYTSYGLSDCVSLSFGTWFPFGLATKWDEPDFRGRYLSQYVDLKQFA
                     AGLQLAWQINDVIAIGAGPEMRFSTVKLHRKVPLFNPFTNRVVDAAHADIRADLEMDV
                     TFGAGIRITPTPKLSIGASYHGQVDANFEGESTFYQISTGNPQLDAAFAAKIPVNTKV
                     PTKTTIQAPAQTQVGIGYDFGK"
     gene            complement(4326..5855)
                     /gene="gcvPB"
                     /locus_tag="P5164_11970"
     CDS             complement(4326..5855)
                     /gene="gcvPB"
                     /locus_tag="P5164_11970"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HRY44650.1"
                     /translation="MSDHGNLTNPVPAALADRRRKSSSIFDAFRPGRKGVLLPALDVP
                     AADPEALFGALHRAEVEGEVEASEVDAIRHFTRLSQKNMSIDANLYPLGSCTMKYNPR
                     INEELARLPGFADVHPYTPEHLSQGALELLARLEEALVALTGFARVTLQPSAGANGEL
                     TACLMIRAAHVKAGAPRKYVLVPDSAHGTNPASAHFAGYAVQELASNPRGTLDLGELE
                     KKMTDEVAAVMITVPNTLGVFEENILEVTKIVHAKGGYVYLDGANFNAFVGKVRPADL
                     GADAMHMNLHKTFSTPHGGGGPGSGPVGVAPALVPFLPVPTVEGADDGWRLEFDRPDS
                     IGRMRTFYGNYGVLVRALTYILSYGNEIAEVAETSVLNANYLRKKLAPFYHLKYDAPS
                     YHEVVFDDKLQAAKGVHNIDVAKRLMDYGFHPPTMSFPLIVPGALMIEPTESEPVEEL
                     DAFADAMIAIAKEAEESPELVTSAPHVTPVGRIDEALLAREASKPGSFAPVLRAPVRR
                     S"
     gene            complement(5866..>7055)
                     /gene="gcvPA"
                     /locus_tag="P5164_11975"
     CDS             complement(5866..>7055)
                     /gene="gcvPA"
                     /locus_tag="P5164_11975"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HRY44651.1"
                     /translation="MAVKNAAATCFASFLGAGLYGHHSPAFVSQLLLRGEFLTSYTPY
                     QPEVSQGTLTAIWEFQSHIALLTGLDVANASMYEGATAFVEAVLMAERLTKKRTKVVV
                     SRTIHPEYLATLKTYLAYLGLEVAEVAAGADGVTSAAALAAAVDETTFAVAVQSPNVF
                     GLVEDWTVASEAAHAHGALAIGVVNEAFSLALLTPPGAAGIDIACGEAASFGVPASFG
                     GPLIGFLSCRDAFKRQIPGRLAGRLETPDGPVFALTLSTREQHIRREKATSNICSNQG
                     LIALMATMTLTALGKHGLRETALQCLSKSEYLKTGVKALDNGKFAVAFPGATFNEFAL
                     RCGPPVAEVLAALEKAQILGGVPFARLAPGAGEHEDLLLVATTERVRREDLDRYLDVL
                     KGF"
BASE COUNT          982 a         2241 c         2657 g         1075 t
ORIGIN      
        1 gccgacctcg ccgatctgct gcccgcgctt caccgtgtct ccgaccttca ggccgggcgc
       61 gtagcgggag aggtgcgcgt agtagtagac gaaccgaccg ctcgcgtccc gctggtagat
      121 cacgatgccg ccgcgccggt cgcgcccgag gcgctcgatc ttcccgtcgc tcacggccac
      181 gatcggggtc cccttcgggg cgccgaggtc gatcgcgtgg tgcctcttgt ggcggccgcg
      241 ggacgcgtca aaggtgtcgc ggagcttctc cttcgggaac gcctcgatcg ggagcgggaa
      301 cccggtcgcg aagagcgcgg cgagctcctc ctcggggatc gacgccggct tgctgtcctt
      361 cttgcggacg acgaggccct tcagcgtcga ccggaggtcc cgccagaagg agcgctgctc
      421 ctcggagaga gccggaagcg gcgcggcggg gctcgcgccc gcggcccagc tcccctcctc
      481 gtacggctcc tcgccgtccg ggtcggcctc gagcgggaga tgctcctcgg cctgggtccg
      541 ggcgacggcg aggacggccg gcaggccggg gggaagggcg accttcggtg cgcgcggcgc
      601 ggcggcgctg gcccgggcga tccaggcgag gtcggcctca tccgcggagg agaagcagcc
      661 gccgtcctcg gggtcgcgga acgggcaggg tgcgggagaa gcggaaggcg cccgcgcggc
      721 ctcggccagg accgaaggag cgccctcgcg cgcggtcttc gcggcggccg cttcggaccc
      781 cgccgcgagg gcgagcccgg cgagggccac ggccgcggag agcgcggtcc tggtcagccc
      841 gccctctcca ggtaggagtc gtcgcgcgtg tcgatcttga ttaccgtacc cgcctcgacg
      901 aactggggga tctggacgat gaggccggtc tcgagcttgg ccggcttgta ggaggcggaa
      961 gccgtcgccc ccttcatccc gggctccgtc tcgacgacct tcaggttcac cgtcgtcggc
     1021 agctccaccg agatcgggtt cccgtcgtag aagtcgacgt cgatgaccgt ctcggggagg
     1081 aggtacttca tggcgtcgcc gacgatctcg tccgagaggc ggacctggtc gtaggtctcc
     1141 tggttcatga agtggtgccc ctcgccgtcc gagtacaggt actgcatctc gtgcgtgtcg
     1201 agggtcgcct tctcgatctt gtcctcggag cggaagcggt gctcgttcat cgcgccggac
     1261 ttgaggctcc ggagcttcgc ctggacgaag ccgcggaggt tcccgggcgt cttgtggacg
     1321 accgagtggg ctcggtgcag ctccccgttg tggatgacga tcatgccggc gcgaagttcg
     1381 gtagctgaga tcatgccgcg gcctggtgca acccttcctg cgggcacggc ttgcgccgga
     1441 cgaaccggac ctgccgcgcc gaacccgcga agggtaatcc cagtgccggc cccaaagcaa
     1501 gcgggaccgc ccgagggcgg tcccgcctgg aagtcgttgg tggcgaaggt ttagtcgtcg
     1561 attcgggcgc tccggccgcg tggctcggcc ggcacggggg cctcttcggc cgccggagcg
     1621 gccaccgcgg tgcgcgcgga gacggggaag gtttcggcga gggtccgcat cacctcgggc
     1681 gtgtagacgg cggcgcccca ggcgaccgcc tcctcggtcg tcaggttcgc gccgaggttg
     1741 acggggtatc cgccctgcga agagttgccc gagaagaggt agcccgagat gtcgacccgc
     1801 gggatcgtcg tgccgtagtt cgcgttgatg aaccggacca tctcgtcggc ccagacggcg
     1861 tagccgaggg aagtcgggtg gacgccgtcg taggagatca gccccccgct caggaacgcc
     1921 gcgttcacgg tgatcccggc gtattcgcgg ccgtgggtcc ggaggtcggt gaagaacgcg
     1981 gtcgtgtcga acaccttgta gccggccgcg gtcgcgatcg accggatctg cgcgttgaac
     2041 tccgcgccgc gggtcttcag gagcgcgacc tcctcgggat agaggacgac gcccgggatt
     2101 ccgagctggg cgttcgcgtt gtccggcagg ggcttgttgc agttcgtccg ccgcgggtcg
     2161 ttcgcgggag cgccgccggc gtcgaggacc gcgcagggga tgccgtaccc ggtcgcgagg
     2221 aacgccgagg ccgtgatgtt gacgaggctc gtcgccggga tcggcgcgac gcccgcgccg
     2281 ccgcccgtga cgtctccgcc gcgcgagctc aggaacgtga ggggctggcc gttcgggccc
     2341 atgacgacct ggccgcccga ggtgaggaac ggcgggatcg tggaggtgaa ggggatcgtc
     2401 gtgatgtcgg ggatcagctg ggcgacgccc ttcccggtcc cgccctgggc ggccttgagg
     2461 gtgttcacga ccgcctggta gctggcggcg tacatcgcgg ccggcatgac ggtgacgccg
     2521 tcgacggccg tgcccgaggt cgccgggccg aggatctcgt tcccgaagag cccgatcgag
     2581 acgaacgtcg gcttcagcgc cgccgcctgc tcgacctgcg tcgcgttcag cgtccggagg
     2641 acgaggccgg cgacgtcgga cgcgctcgag gggcccttct tcgtgagggc cgcctggagc
     2701 gtgtagcccg ggatcgagag gttgtcgtag ggacggggga ggttcaggtt cagcggcttg
     2761 atctgcgccg gccgcgtccc gaactgcggg gcgaggctga tgaggtacat gcagcccgtg
     2821 ccgggcgtgt cgaggagcgg ctgctcgaac gcgggcgcgc cggcctgccg ggcgaggatc
     2881 gcgttccagg agtcgacctg gccgtagcgg acccagcagt tgtcgaggaa tcccgcgtcg
     2941 accgaggcgc cgatcgctac gaacttcgag aagtcggcct gcgcggcggc cggcgaggcc
     3001 gtcgcggcga gcgcaaggac gagggcgagg aggagggtgg tcttggtcgt cttcatcggt
     3061 caccccctca gaaggacgtc cgcatgttca ggacggtgag gttcgcgaag ttcttccacc
     3121 ggccgttgaa gttgtcgtcg ttcgtcgtga cggagcgctc gctgaannnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnncttg ccgaagtcgt agccgatgcc gacctgggtc
     3301 tgggccgggg cctggatcgt cgtcttcgtc ggcaccttcg tgttgaccgg gatcttcgcg
     3361 gcgaaggcgg cgtcgagctg ggggttcccc gtggagatct ggtagaacgt ggactccccc
     3421 tcgaagttcg cgtcgacctg cccgtggtac gaggcgccga tcgagagctt cggggtcggg
     3481 gtgatcctga tgccggcgcc gaacgtcacg tccatctcga ggtccgcgcg gatgtcggcg
     3541 tgagcggcgt cgacgacgcg gttcgtgaac gggttgaaga gcgggacctt ccggtggagc
     3601 ttcacggtcg agaaacgcat ctcggggccg gccccgatcg cgatcacgtc gttgatctgc
     3661 caggcgagct ggaggcccgc ggcgaactgc ttcaggtcga cgtactggga gaggtagcgg
     3721 ccgcggaagt ccggctcgtc ccacttcgtc gcgaggccga acgggaacca ggtcccgaaa
     3781 gagaggctga cgcagtcgct caggccgtag gaggtataga agttcggcgg gaagaagaac
     3841 tggtccgcca tcttgaccga gtagtcgctg ccggggtacg ggtcgtaccc ctgggcgagc
     3901 tccgccttcg ggacgatcag cgtcaccccg gccatggccc ggagctcccc cttcttgacc
     3961 ccctgggcga ggcccgccgg gttgtagaac atggccgaca ggtcgtccgc gtgcgcgacg
     4021 aacgcgccgc ccatcgcggt cgcgcgcgag ccggcttcga agatggagaa cccggcggcg
     4081 gacgccgggc ctgcggcgac cagcgagatg gccagaagga gcgcgagccc cccggcccgg
     4141 taccgatcac ttcctctcaa cacgaaaccc tcctcattcg cgcttgcgcg atgtgtaaac
     4201 aacttcaagg gcgggcggat tctctctccc gcctgtccag agtcaaggcc cccggaagaa
     4261 gcgccggccc ggcgcgggct cgaaagcccg ggccgggccg gaagaggcgg tgctggagag
     4321 gggccctagg agcggcggac cggggcccgg aggaccggcg cgaagctccc cggcttcgac
     4381 gcctcgcggg cgaggagcgc ctcgtcgatc cggccgacgg gcgtcacgtg cggggccgag
     4441 gtgacgagct cggggctctc ctcggcctcc ttcgcgatcg cgatcatcgc gtcggcgaag
     4501 gcgtcgagct cctcgaccgg ctccgactcg gtgggctcga tcatgagcgc gcccgggacg
     4561 atgaggggga acgacatcgt cggcgggtgg aagccgtagt ccatcagccg cttcgcgacg
     4621 tcgatgttgt ggaccccctt cgccgcctgg agcttgtcgt cgaagacgac ctcgtggtag
     4681 ctcggggcgt cgtacttgag gtggtagaag ggggcgagct tcttccggag gtagttcgcg
     4741 ttcaggacgg aggtctcggc gacctcggcg atctcgttgc catacgagag gatgtacgtc
     4801 agcgcgcgga cgaggacgcc gtagttgccg tagaacgtcc gcatccggcc gatcgagtcg
     4861 ggccggtcga actcgagacg ccagccgtcg tccgcgccct cgacggtcgg gaccgggagg
     4921 aacggcacga gcgcgggcgc cacgccgacg ggacccgagc cggggccgcc gccgccgtgg
     4981 ggcgtcgaga acgtcttgtg gaggttcatg tgcattgcgt cggcgccgag gtcggcgggg
     5041 cggaccttcc cgacgaaggc gttgaagttc gccccgtcga ggtagacgta gccgcccttc
     5101 gcgtggacga tcttcgtgac ctcgaggatg ttctcctcga agacgccgag cgtgttcggc
     5161 accgtgatca tcacggcggc gacctcgtcg gtcatcttct tctcgagctc tccgaggtcg
     5221 agcgtccccc gcgggttcga ggcgagctcc tggaccgcgt agccggcgaa gtgggccgag
     5281 gccgggttcg tgccgtgggc cgagtcgggg acgaggacgt acttgcgcgg agcgcccgcc
     5341 ttcacgtggg cggcgcggat catcaggcag gccgtcagct cgccgttcgc gccggcggag
     5401 ggctggaggg tgactcgggc gaagccggtc agcgcgacga gggcctcttc gaggcgggcg
     5461 aggagctcga gcgccccctg ggagaggtgc tccggcgtgt aggggtggac gtccgcgaac
     5521 ccggggaggc gggccagctc ctcgttgatg cgcgggttgt acttcatcgt gcacgaaccg
     5581 agcgggtaga ggttcgcgtc gatcgacatg ttcttctgcg agagccgcgt gaagtggcgg
     5641 atcgcgtcga cctccgacgc ctccacctct ccctccacct cggcgcggtg gagcgccccg
     5701 aagagcgcct ccgggtcggc cgcggggacg tcgagggccg ggaggaggac gcccttccgg
     5761 ccgggccgga aggcgtcgaa gatcgaggag gacttccggc ggcggtccgc gagggcggcg
     5821 gggacggggt tcgtcaggtt tccgtggtcg ctcatcgtcg gctcctcaga accccttcag
     5881 gacgtcgagg tagcggtcga ggtcctcgcg ccgcacccgc tcggtcgtgg cgacgaggag
     5941 gaggtcctcg tgctcgccgg cgccgggcgc gaggcgggcg aacgggacgc cgcccaggat
     6001 ctgcgccttc tcgagcgcgg cgaggacctc cgcgacggga ggcccgcagc ggagggcgaa
     6061 ctcgttgaac gtcgcgccgg ggaaggccac ggcgaacttc ccgttgtcga gcgccttcac
     6121 tcctgtcttc aggtactcgg acttcgagag gcactggagg gccgtctcgc gcaggccgtg
     6181 cttgccgagg gcggtgagcg tcatggtcgc catgagcgcg atcagcccct ggttcgagca
     6241 gatgttcgag gtcgccttct cgcgccggat gtgctgctcg cgggtcgaga gcgtcagcgc
     6301 gaagaccggg ccgtcgggcg tctcgaggcg gccggcgagg cggcccggga tctgccgctt
     6361 gaacgcgtcg cggcaggaga ggaacccgat gagggggccg ccgaaggagg cggggacgcc
     6421 gaaggaggcc gcctcgccgc aggcgatgtc gatgcccgcc gcgccggggg gcgtcaggag
     6481 ggccagggag aacgcctcgt tcacgacgcc gatcgcgagc gccccatggg cgtgggccgc
     6541 ctcggaggcg acggtccagt cctccacgag gccgaagacg ttcggggact ggacggcgac
     6601 cgcgaacgtc gtctcgtcga ccgcggcggc gagggcggcg gcgctcgtca cgccgtcggc
     6661 gccggccgcc acctcggcga cctcgaggcc gaggtaggcg aggtaggtct tcagcgtcgc
     6721 gaggtactcc gggtggatcg tccgcgagac gacgactttc gtccgcttct tcgtcagccg
     6781 ctccgccatc aggacggcct cgacgaaggc cgtggccccc tcgtacatcg acgcgttcgc
     6841 gacgtcgagg cccgtcagga gcgcgatgtg cgactggaac tcccagatcg ccgtcagcgt
     6901 cccctgggag acctcgggct ggtagggcgt gtacgacgtg aggaactccc cgcggaggag
     6961 gagctgggag acgaacgccg gcgaatggtg gccgtagagg ccggcgccga ggaaggaggc
     7021 gaagcaggtc gcggcggcgt tcttcacggc catcg
//
LOCUS       DAOTEZ010000216         7031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_7645_length_7031_cov_6.795872, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000216 DAOTEZ010000000
VERSION     DAOTEZ010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7031
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7645_length_7031_cov_6.795872"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="P5164_11980"
     CDS             complement(<1..656)
                     /locus_tag="P5164_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44652.1"
                     /translation="MVRRTFVLAAALLWAIPVPRTAEGQEPAAIRVERLPASLAGTWE
                     FALGDPPGGIADLDALPFRPIAVPGSWQAAGFPGHGTGWYRVPIDLDPSLSIVPLAFA
                     CSQIRDVDEVYLDGQPIGRTGGFPPAYDKGTVVERIYELPPSRSSVPGRHVLAVRVYN
                     AGPRGGGITGTPRLVAVSAAFHARTMREAPRALLAAAFGALGLFSLFFFLRDRGQTDF
                     L"
     gene            749..1459
                     /locus_tag="P5164_11985"
     CDS             749..1459
                     /locus_tag="P5164_11985"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY44653.1"
                     /translation="MELRDSVALVTGAGRGIGRAVALAFAREGARVALMGLTASHLSA
                     VEKEAGALGARCAVLPGDVSDEGSVARCVAAAEQQLGPIDVLVNNAGVYAHGPVDRLD
                     ALVFDHTLAVNLRGPFLMARAVLPGMKSRRRGHVVNVASTAALRGFAGGAAYCASKFG
                     LAGLSQAMLQDVRGSDVRVTCILPSTVATDMALRGDFLKSPATAIRPEDVAQAVVDLI
                     RLDSRVLPVSLELWQTNP"
     gene            complement(1482..3029)
                     /locus_tag="P5164_11990"
     CDS             complement(1482..3029)
                     /locus_tag="P5164_11990"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRY44654.1"
                     /translation="MSGEDLTASRVRRMLPSVSEVLRELTARAPIEPDVAFRAARDVC
                     AEELKRIRDAGGESVPMELLVERALARVAGVPPPLVVPAPPTFVPAEPSEPEPLPAAG
                     PTAAEDPFSETTGALDLRWDRDTREPLAAPLAAASAEEELPLPHLVESAPEPEPEEEE
                     RPLPPHADELPAPAAAFAGEPFAAADEEPAAEGAAFAMAGPEEDVPLPEPPPAGAGAE
                     EEFGDETLTRLSREAEAIDLRSVLAGGGPGLQPQEEGYDAAPTASVVDAKAEPAEPPP
                     PPFEDDEDTVLTVPRGGRRRAKGEDGPRWGRLAGIAAALAALAALGYLLVGLWIDRGA
                     DSGRVAVARRVPEESGAPAPGASPTPVPEASPAPLPAGGATASVPAESAGPTTPAVAR
                     PDAVPTAAPAPPSPSPAVAAAAPVRQSRDGLVVTRDRAGRPEVFSIHFTSYRDRAAAE
                     RDLKRVEALAGREGYVAEVDLGEKGIWFRVLVGEFPSAEEAKGVRAELAAKGTRDMGW
                     VYRVVGP"
     gene            complement(3026..4042)
                     /locus_tag="P5164_11995"
     CDS             complement(3026..4042)
                     /locus_tag="P5164_11995"
                     /inference="COORDINATES: protein motif:HMM:NF018435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRY44655.1"
                     /translation="MSGKKDVLHVGIDLGTSRSAIAASNGERHVVESWVGWPVDMVAR
                     KVVKKPVVFGHEALENRPMLDLHRPLERGLIKEGSPKAEAAVRELLAHLIEKAGGAGK
                     TVQAVVGVPAEAMRVSRQHLRNAVKGVADALLLVSEPFAVAYGMDALLHTMIIDIGAG
                     TSDFCVMNGRYPTDDDQRTLTHAGDTVDEQLGLQIRARHPEANFSIHMVREWKEKSSF
                     VGEPREPVFVEAPVRGKPTRLEVTAEMRKACESLVGPLAETMLDLLSRVDPEYQQRVR
                     NNVVLAGGGSQIPGLAAALERALDDVGGGKVRPVDDPVFAGANGSLSIAADAPEGDWE
                     RLAG"
     gene            complement(4039..5007)
                     /locus_tag="P5164_12000"
     CDS             complement(4039..5007)
                     /locus_tag="P5164_12000"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRY44656.1"
                     /translation="MRENLDAIEGRPPARLRPGGRWARRAVRGAALVAIPLALFASIN
                     AFSGGGASPTEVVVVPQRTVAPSPEPGLETLDWPAPKSIDPTLFHLDVRTIVLDAGHG
                     GVDPGALTADGLAEKDVTLDVARRVREKLRGGLFRVVMTREGDETISLRERSRRARDA
                     GGDVLVSIHVNSIPGPDRRGVETYYLGPTDDPAIRRLASAENHSSGYSLSDFRGLLEE
                     VYVGVRQAESRRLAESVQAELVASTRRVTPEAGDRGVKSAPFVVLIASEMPGILAEVS
                     SVSHAEDARLLRDEAYREAIAEALARGVRAYADARNLADAGRSENG"
     gene            complement(5109..5723)
                     /locus_tag="P5164_12005"
     CDS             complement(5109..5723)
                     /locus_tag="P5164_12005"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRY44657.1"
                     /translation="MARIYDLSPPLSPRIAVWPGDVPFSREVTTPPDGTTFSSIRTTL
                     HLGSHADAPLHMVAGAPPIDAVPLETWIGPCQVVEARVAPRGRVRLEDLVEPPAAPRL
                     LLKTGTSGSRERFDDAFAGLDPSLASELAARGVVLVGIDTPSVDPYDDHAYAAHRALV
                     RAGVAWLEGLVLAHVPPGRYELSALPLRIEGGDGSPVRAVLVAR"
     gene            complement(5734..6597)
                     /locus_tag="P5164_12010"
     CDS             complement(5734..6597)
                     /locus_tag="P5164_12010"
                     /inference="COORDINATES: protein motif:HMM:NF012571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HRY44658.1"
                     /translation="MSEATRKIDAAARGASPHAGGTEELVPLDPDHPGFKDPVYRARR
                     NAIAKLALDYREGQPLPDVEYTEDEQAVWRTVWEHLAPLHERFACREWTESSKALALD
                     RTRVPQLAEVNEALKRGAGFRMLPVAGLVQGRTFLGMMGKGAFLSTQYMRHHSVPLYT
                     PEPDVVHELVGHAASLFHPGIVTLSRRFGEAAWRVDDAALARLEYLYWYTLEFGLVEE
                     DGERKAVGAGLLSSFGELGSFEANAEIRPLDCDAAAAIPYDPTKYQELLFVSPSFDRM
                     VEDVSAWLERQ"
     gene            complement(6617..6817)
                     /locus_tag="P5164_12015"
     CDS             complement(6617..6817)
                     /locus_tag="P5164_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44659.1"
                     /translation="MAKQRSDEEQKAVETILRALAHRLQRLRDEAAMQALRGETDPGV
                     EKGLDMALRELNDLLPRRKRVK"
     gene            6866..>7031
                     /locus_tag="P5164_12020"
     CDS             6866..>7031
                     /locus_tag="P5164_12020"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRY44660.1"
                     /translation="MSVDVLLVRTHSPGNLGSAARACKAFGAGLLLLDPRADAAHEDA
                     RAFASGAEDLL"
BASE COUNT          917 a         2637 c         2534 g          943 t
ORIGIN      
        1 aggaagtccg tctgcccccg gtcgcggagg aagaagaaga gcgagaagag cccgagcgcg
       61 ccgaacgccg cggcgaggag ggcgcggggc gcctcgcgca tcgttcgggc gtggaaggcc
      121 gcggagaccg ccacgagccg cggcgtcccg gtaatcccgc ctccgcgcgg cccggcgttg
      181 tagacgcgca cggccaggac gtgccgtccc gggacggacg accgcgacgg gggaagctcg
      241 tagatccgct ccacgacggt ccccttgtcg taggccggcg ggaaccctcc ggttcgtccg
      301 atcggctgcc cgtcgaggta gacctcgtcc acgtcccgga tctgcgagca ggcgaacgcg
      361 agcgggacga tcgagagcga ggggtcgagg tcgatcggga cccggtacca ccccgttccg
      421 tgaccgggga agcccgcggc ctgccagctc cccggcacgg cgatcgggcg gaacgggagc
      481 gcgtcgaggt ctgcgatgcc gccgggaggg tctccgagcg cgaactccca ggtcccggcg
      541 aggctggccg gcaggcgttc gacccgtatc gcggcgggct cctgcccctc cgccgtgcgc
      601 ggcacgggaa tggcccagag gagcgccgcc gccagaacga acgttcgccg gaccatccgg
      661 gccagcatct cccatccgcg acgaaggggc aacgaccgcc gtaaggcccg gtcggcgggg
      721 cggataagat cgtcgccgga ggcccgccat ggagctcagg gattcggtcg ccctcgtcac
      781 cggcgccggc cgtgggatcg gccgggccgt cgccctcgcc ttcgcgcgcg aaggggcccg
      841 cgtggccctg atgggcctga cggcgtcgca cctctcggcc gtcgagaagg aggcgggggc
      901 gctcggcgcg cgttgcgccg tcctccccgg cgacgtctcc gacgagggga gcgtcgcgcg
      961 ctgcgtggcc gcggccgagc agcagctcgg gccgatcgac gtcctcgtca acaacgccgg
     1021 cgtctacgcg cacggcccgg tcgaccggct cgacgcgctc gtcttcgacc acacgctcgc
     1081 ggtcaacctc cgcggaccgt tcctgatggc gcgcgccgtt ctcccgggga tgaagagccg
     1141 gcgccgcggc cacgtcgtca acgtcgcctc cacggcggcc ctgcgcggtt tcgccggggg
     1201 agcggcctac tgcgcctcga agttcgggct cgccggcctc tcgcaggcga tgctccagga
     1261 cgttcggggg agcgacgtgc gcgtcacctg catcctcccc tcgacggtcg ccaccgacat
     1321 ggccctccgg ggcgacttcc tgaagagccc ggcgacggcg atccgccccg aggacgtggc
     1381 ccaggcggtc gtcgacctca tccggctcga ctctcgcgtc ctcccggtct ccctggagct
     1441 ctggcagacg aatccctgaa cgcgcggccc cgcggcggga ctcaggggcc gacgaccctg
     1501 tagacccacc ccatgtcgcg cgtccccttc gccgcgagct ccgcgcgaac gcccttggct
     1561 tcctccgccg acgggaactc cccgaccagg acgcggaacc agattccctt ctcgccgagg
     1621 tcgacctccg cgacgtagcc ctcgcggccg gcgagcgcct cgacccgctt caggtcgcgc
     1681 tcggcagcgg cccggtccct gtaggacgtg aagtggatcg agaagacctc ggggcgtccg
     1741 gcccggtccc gggtgacgac gaggccgtcg cggctctggc gaaccggtgc ggccgccgcg
     1801 acggcgggcg agggcgacgg cggtgcgggc gcggccgtcg ggacggcgtc cgggcgcgcg
     1861 acggcgggag tcgtcggccc ggccgattca gcgggcaccg aagccgtcgc gccgcccgct
     1921 ggcagagggg ccggcgacgc ctcgggcacc ggcgtcgggg acgcccccgg agccggcgct
     1981 ccggactcct ccgggacccg ccgggccacg gccacccgtc cggagtccgc tccccgatcg
     2041 atccagagtc cgacgagcag atagccgagc gccgcgagcg ccgcgagcgc cgcggcaatc
     2101 ccggcgagac gtccccatcg cggcccgtcc tcgcccttcg ctcgacgtcg tccgccgcgc
     2161 ggaaccgtga ggacggtgtc ctcgtcgtcc tcgaagggag gcggaggagg ctcggccggc
     2221 tcggccttcg cgtcgacgac cgacgccgtc ggagcggcgt cgtatccctc ctcctgcggc
     2281 tgcagaccgg ggcctccgcc cgcgaggacc gagcggaggt cgatcgcttc ggcctcccgg
     2341 gagagccgcg tgagcgtctc gtctccgaac tcctcctccg ctccagcgcc cgccgggggc
     2401 ggctccggga gaggcacgtc ctcctccggc ccggccatcg cgaaggccgc accctcggcc
     2461 gccggctcct cgtcggctgc cgcgaagggc tcccccgcaa acgccgcagc aggcgcgggg
     2521 agctcatccg cgtgaggcgg gagcggccgc tcctcttcct cgggttcggg ctccggggcc
     2581 gactccacga ggtgcgggag cggcagctcc tcctccgcgg acgcggcggc gagcggcgcg
     2641 gcgagaggct ctcgcgtatc ccggtcccat cgcaggtcca gggcgcccgt cgtctcggag
     2701 aacgggtctt cggcggcggt cggacccgct gcgggaaggg gctccggctc ggacggctcg
     2761 gcggggacga acgtcggcgg ggccggcacg acgagaggtg gcggcacgcc ggcgacgcgc
     2821 gcgagcgccc gctccacgag gagctccatc ggaacgctct ccccgccggc gtcgcggatc
     2881 ctcttcagct cctccgcaca aacgtcccgc gccgcgcgaa acgccacgtc gggctcgatc
     2941 ggcgcgcgcg ccgtgagctc gcggaggacc tccgagaccg acgggagcat gcgcctcacg
     3001 cgagaggcgg tgaggtcttc gccgctcatc cggcgagtcg ctcccagtcc ccttcgggag
     3061 cgtcggcggc gatcgacagg gagccgttcg cacccgcgaa gaccgggtcg tccacgggcc
     3121 tcacctttcc cccgcccacg tcgtcgaggg cccgctcgag cgccgccgcg aggccgggga
     3181 tctgggagcc gccccccgcg aggacgacgt tgtttcgcac cctctgctgg tactcggggt
     3241 cgacgcgaga caggaggtcg agcatcgtct cggcaagggg tccgacgaga ctctcgcacg
     3301 ccttcctcat ctccgccgtt acctcgaggc gcgtcggctt cccgcggacc ggggcctcga
     3361 cgaacacggg ctccctcggc tcgccgacga agctgctctt ctccttccac tcgcggacca
     3421 tgtggatcga gaagttcgcc tcggggtggc gagcccggat ctggagcccc agctgctcgt
     3481 cgaccgtgtc gcccgcgtgc gtgagcgtcc tctggtcgtc gtcggtcgga tagcgcccgt
     3541 tcatgacgca gaagtcgctc gtccccgcac cgatgtcgat gatcatcgtg tggagcagcg
     3601 cgtccatgcc gtaggcgacg gcgaacggct ccgacacgag aaggagcgcg tcggccactc
     3661 ccttcacggc attccgcagg tgctgcctcg agacgcgcat cgcttcggca gggacgccga
     3721 cgacggcctg gaccgtcttc ccggcgccgc cggccttctc gatcaggtgc gcgaggagct
     3781 cccgcacggc cgcctcggcc ttcggggagc cctccttgat gagccccctc tcgagggggc
     3841 ggtggaggtc gagcatcggc cggttctcga gcgcctcgtg cccgaagacg accggcttct
     3901 tcacgacctt ccgggcgacc atgtccaccg gccagccgac ccagctctcc acgacgtgcc
     3961 gctcgccgtt cgaggcggcg atcgcgctgc gcgacgtgcc gaggtcgatc ccgacgtgga
     4021 ggacgtcctt cttgccgctc atccgttctc gctccttccc gcatccgcga ggttgcgcgc
     4081 gtcggcgtag gcgcgcacgc cccgcgcgag cgcttccgcg atcgcctccc ggtaggcctc
     4141 gtcgcggagg aggcgggcgt cttccgcgtg cgagacgctc gagacctccg ccagtatccc
     4201 cggcatctcg gaggcgatca ggacgacgaa cggggcgctc ttcacccccc ggtcgccggc
     4261 ctccggcgtg accctccgcg tcgaggcgac gagctcggcc tggaccgact cggcgagccg
     4321 tctcgactcg gcctggcgaa cgcccacgta gacctcctcg agaagtcccc tgaagtccga
     4381 cagagaatag cccgaggagt ggttctcggc gctcgcgagc cgacggatcg ccggatcgtc
     4441 ggtcgggccg aggtagtagg tctcgacccc gcggcgatcg ggccccggga tcgagttgac
     4501 gtggatcgag acgaggacgt ctccgcccgc gtctcgcgcc cttcgcgaac gctcgcgcag
     4561 ggagatcgtc tcgtctccct cccgggtcat cacgacgcgg aagaggcccc cgcggagctt
     4621 ctccctcact ctccgcgcga cgtcgagggt cacgtccttc tcggcgaggc cgtcggccgt
     4681 cagagcgccg gggtcgacgc cgccgtgtcc cgcgtcgagg acgatcgtcc tcacgtcgag
     4741 atggaagagg gtcgggtcga tcgacttcgg cgcgggccag tcgagcgtct cgagccccgg
     4801 ctcgggcgag ggagcgaccg tccgctgcgg cacgacgacg acctccgtcg gggaggcgcc
     4861 cccgccggag aaggcgttga tcgaggcgaa gagcgcgagc ggaatcgcga ccagggccgc
     4921 gccgcgcacg gccctgcggg cccaccgccc ccccgggcgg aggcgcgccg gaggccgtcc
     4981 ctcgatcgcg tcgaggttct cccgcacggc gtcgcggacg catctgcctc ttcagccgct
     5041 cggcgtctcg catggcgaac ttctcatttt cgcagctttt ccggtgcgcg gcgggccgcc
     5101 ccgggcgatc agcgcgccac gaggaccgcc cggacgggag agccgtcccc tccctcgatc
     5161 cggagaggca acgccgaaag ctcgtatcgg cccggcggga cgtgggcgag gacgagcccc
     5221 tccagccacg cgaccccggc gcggacgagc gcccggtgcg ccgcataggc gtggtcgtcg
     5281 tacgggtcga cggagggggt gtcgatcccg acgaggacga cgccgcgcgc cgcgagctcc
     5341 gaggcgagcg acgggtcgag acccgcgaac gcgtcgtcga accgctcccg gctcccggac
     5401 gtcccggtct tcaggaggag ccgcggcgcg gccgggggct cgacgaggtc ctcgaggcgc
     5461 acgcggcctc gcggcgccac gcgcgcctcc acgacctggc acggcccgat ccaggtctcg
     5521 agggggacgg catcgatcgg gggcgctccg gccaccatgt ggagcggcgc gtcggcgtgc
     5581 gagccgaggt ggagcgtcgt ccggatcgac gagaaggtcg tcccgtcggg aggcgtcgtc
     5641 acctcccgcg agaacggcac gtcgcccggc cagacggcga tccgcggcga cagcggcggg
     5701 gagaggtcgt agatgcgagc catcggtccc ggctcactgc cgctcgagcc aggccgagac
     5761 gtcctcgacc atccggtcga aggaggggga gacgaagagg agctcctggt acttcgtcgg
     5821 gtcgtacggg atcgcggccg ccgcgtcgca gtcgagcggc cggatctcgg cgttcgcctc
     5881 gaacgagccg agctcgccga acgaagaaag gaggccggcg ccgaccgcct tccgctcccc
     5941 gtcctcctcg acgagtccga actcgagcgt gtaccagtag aggtactcga gccgagccag
     6001 cgccgcgtcg tccacgcgcc acgccgcctc gccgaagcgc cgcgagagcg tcacgatgcc
     6061 ggggtggaag aggctcgccg cgtggccgac gagctcgtgg acgacgtcgg gctcgggcgt
     6121 gtagagcggc acggagtggt gccgcatgta ctgcgtcgag aggaaggccc ccttccccat
     6181 catcccgagg aacgtccgcc cctggacgag gccggccacg gggagcatcc ggaagccggc
     6241 gcccctcttc agcgcctcgt tcacctcggc gagctgggga acgcgcgtcc ggtcgagcgc
     6301 gagggccttc gagctctccg tccactcccg gcacgcgaac cgctcgtgga gcggcgcgag
     6361 gtgctcccag acggtccgcc agacggcctg ctcgtcctcg gtgtactcca cgtcgggcag
     6421 cggctggccc tcgcggtagt cgagcgcgag cttcgcgatg gcgttccgcc gcgcccgata
     6481 gaccgggtcc ttgaagcccg ggtggtccgg gtcgagcggg acgagctcct ccgtaccccc
     6541 ggcgtggggg gaggcgccgc gcgccgccgc gtcgatcttc cgagtcgcct cgctcatcgc
     6601 cccgtcgtcc cgcccgtcac ttgacccgct tccggcgcgg caggaggtcg ttcagctccc
     6661 gcagggccat gtcgagcccc ttctcgacgc ccgggtcggt ctcgccccgg agcgcctgca
     6721 tcgccgcctc gtcgcgaagg cgctggaggc ggtgcgccag ggcccggagg atcgtctcga
     6781 cggccttctg ctcctcgtcg ctgcgttgtt tcgccacgca ggcattctgg acgagaccgg
     6841 cgccgctttg gaagatgatc cggcggtgag cgtcgacgtc ctcctcgtcc gcacccacag
     6901 ccccgggaac ctcggctcgg cggcccgcgc ctgcaaggcg ttcggcgcgg ggctcctcct
     6961 cctcgacccg cgcgccgacg ccgcgcacga ggacgcccgg gccttcgcgt cgggcgcgga
     7021 ggacctcctc g
//
LOCUS       DAOTEZ010000217         6931 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_7782_length_6931_cov_6.082752, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000217 DAOTEZ010000000
VERSION     DAOTEZ010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6931
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7782_length_6931_cov_6.082752"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /gene="pip"
                     /locus_tag="P5164_12025"
     CDS             <1..668
                     /gene="pip"
                     /locus_tag="P5164_12025"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HRY44661.1"
                     /translation="LREHLGIAKWQVFGGSWGSTLALAYAERHPDRVTELVLRGIFLL
                     RRKEIEWFYQEGASILFPDAWEPYLAHIPVEERGDLVAAYHRRLTSDDPAVRLAAAKI
                     WSGWEGATSKLLPDAAFTGHYEEDEFALAFARIEAHYFQNRGFFEADDQLLRDVGRIR
                     HIPGVIVQGRYDVVCPIFSAWALHRAWPEAELIITPDSGHSAFEPGNSRALVAATDRF
                     AAL"
     gene            796..1893
                     /locus_tag="P5164_12030"
     CDS             796..1893
                     /locus_tag="P5164_12030"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRY44662.1"
                     /translation="MLPASLGLALVALLFRLVSPAEIRALLDLHLLSLFLVLILAVEC
                     LKRSGLIEVVVAKVLSRVRSERALAAAAIVTTGAVSAMVTNDVALLLVVPFTLAFEKV
                     APELDPVRVVVLEIQAANLLGCLTPTGNPQNLLLFSRGGFTTGSFFAAQLPWVIGMAA
                     ALLALVPILVPRRALPAPPPAPRAVEPRLATAGLFLLSLQLLAISQAIPRLAPLLAAL
                     PAAALLGKGLLKTDFTLLGVFSALFVGVEGLRRSALFETLDPVALFGATPGGFVLSGA
                     LLSQAVSNVPAAILLAPSAAAAGGGALFTALLYGVNAGGCGTPIASLANLIGADLYLR
                     GRAHRAPFWRLFLAVSFALLAVAVALSLALA"
     gene            complement(1930..3207)
                     /locus_tag="P5164_12035"
     CDS             complement(1930..3207)
                     /locus_tag="P5164_12035"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY44663.1"
                     /translation="MSERIFDALRRTFGENLRFASIALRAHKLRAFLTIVGIVIGVWT
                     VIAMVALVTGFNRSTMEAFSSFGTTLVQFQKFEPRFFGGGHRPPEEERNRKDLTIEDA
                     EALARLAPSIAAVSPERYLWGGSKVKGNGKEANSPTVTGGNQNYLVCNNWALADGRNI
                     TDVDVRHATDVAVIGADVVKSLFEGKDPLGQPITINGRRVQVVGLLEKKGSQMGDSPD
                     NIIIIPITTFDVFFPQIKNSRGDTLHIATIPKSPDLIQQAIEEGTNILRARRKVPFDK
                     PNDFAIFTAEKLIAQMTAVTNAISGVMVFIAAIALLVGGVGVMNIMLVSVTERTREIG
                     LRKSVGALRRDISLQFLTEAMTLTGLGGAVGVALGVLTALVVGAVSPLRTATPLWSIL
                     VGLGVSISIGLFFGLYPALKAARLDPIDALRWE"
     gene            complement(3200..4420)
                     /locus_tag="P5164_12040"
     CDS             complement(3200..4420)
                     /locus_tag="P5164_12040"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY44664.1"
                     /translation="MLNGELFRVAWRAISAHKLRSFLTLLGVIIGVMTVIAVVSVISG
                     LNNYVATKLFSLSPDVYTIERFGIITSREEFLDALKRKRIQKSDLEAYRRLAKHAVAV
                     GARSGTTRSVRSGNRRLSDVQIAGVTANVAELSNLDVDLGRFFTESENERRSFAAVIG
                     TEVRDELWPRLDPVGRTISIDGIPFRVIGLLVKQGSVFGQSQDNVVYVPREALAKAWG
                     TRRNISIYVKARGGVEGIPASMDEARAIFRALRKTAASAPDPVSFVTAEAIQVVWRSI
                     SAGAFALLAFISSISLVVGAIVIANIMLVSVVERTKEIGIRLAIGARKRDIRRQFLLE
                     ATLLALGGGAVGVLLGWVISSLINAFTPLPTLVSPTLVTAGLLLATLTGLVAGVFPAV
                     KASRLPPIEALRYE"
     assembly_gap    4602..4701
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4702..5112)
                     /locus_tag="P5164_12045"
     CDS             complement(<4702..5112)
                     /locus_tag="P5164_12045"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRY44665.1"
                     /translation="MSSRLKWILGAVAALVVVLVIVASVAGKDRNLTRVTTAKVGKAD
                     LVSTVSCSGRVQAKTKVEISSQVMGQIINLAVEEGDVVKKGDFLLQIDKAQYDATTKA
                     QQANLEALFAQRESDRATVEQAKRDYDRERKNFEA"
     gene            complement(5252..6169)
                     /locus_tag="P5164_12050"
     CDS             complement(5252..6169)
                     /locus_tag="P5164_12050"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRY44666.1"
                     /translation="MAQIRSRILIVDDDALSRRVLEQVLVAADYEVESAGDGQEALGK
                     VRLHRPDVVVTDWQMPNMDGLTLCRILKGTEETRFTHVIMMSTQGETEAKVTGLETGA
                     DDYLVKPVEPVELKARVKVGLRLQRALSDLASKNEILEKLALTDPLTGLSNRRAFGEA
                     LASELSRIVRHNQPASLLTFDLDHFKQVNDTHGHAVGDEVLAAFAAVLKRGCRRGDLA
                     ARLGGEEFAILLPTTALVPAALVAERIRRATEWRPLGVSVPVPVTVSVGIASTESLPH
                     PPAAAELLRRADAALYRAKDEGRNRVVSG"
     gene            complement(6219..6306)
                     /locus_tag="P5164_12055"
     tRNA            complement(6219..6306)
                     /locus_tag="P5164_12055"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6270..6272),aa:Ser,seq:tga)
     gene            complement(6318..6410)
                     /locus_tag="P5164_12060"
     tRNA            complement(6318..6410)
                     /locus_tag="P5164_12060"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6374..6376),aa:Ser,seq:gct)
     gene            complement(6492..>6931)
                     /locus_tag="P5164_12065"
     CDS             complement(6492..>6931)
                     /locus_tag="P5164_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRY44667.1"
                     /translation="VELVKFTTAEGSAEAHERLTRDAETVLERLGLPYRRVVLCRGDM
                     GFSSAKTYDLEVWLPGQDAYREISSCSNFGAFQARRAAIRVKRTAEGKSRNEFVHTLN
                     GSGLAVGRTLVAILENGQREDGSVAIPEALRPYCGGLAEIRKG"
BASE COUNT          945 a         2400 c         2385 g         1101 t
ORIGIN      
        1 agctccgcga gcacctcggg atcgcgaagt ggcaggtctt cggcggctcc tggggctcca
       61 cgctcgcgct cgcctacgcc gagaggcacc ccgatcgcgt cacggagctc gtcctgcgcg
      121 gcatctttct ccttcggcgg aaggagatcg agtggttcta tcaggaaggg gcgtcgatcc
      181 tcttccccga cgcctgggag ccgtacctcg ctcacattcc cgtggaggag cgcggcgacc
      241 tggttgcggc ctatcaccgg cgcctcacga gcgacgaccc cgcggtccgt ctcgccgcgg
      301 cgaagatctg gagcgggtgg gagggagcga cgtcgaagct cctccccgat gccgccttca
      361 ccggccacta cgaggaggac gagttcgcgc tcgcgttcgc gcggatcgag gcccactact
      421 tccagaaccg cggcttcttc gaggccgacg accagcttct gcgcgacgtg ggccgcatcc
      481 gccacatccc cggggtcatc gtccaggggc gctacgacgt cgtctgcccg atcttcagcg
      541 cctgggccct gcatcgcgcc tggcccgagg cggagctgat catcacaccc gactccggcc
      601 acagcgcctt cgagccgggc aacagccgcg cgctcgtcgc cgcgacggat cgcttcgccg
      661 cgctctgagc ccgacgggac cttcccgtcc ttccgaggtc cggacggggc gccggccgcg
      721 cgcggtatcg gaccagcccg gccggccgca gagtgtagag tgtagaccag acccctcgtc
      781 cggttatgat cgggcgtgct cccggcgtcg ctcgggctgg cgctcgtcgc gctcctcttc
      841 cggctcgtct cgccggcgga gatccgggcg ctgctcgacc ttcacctgct ctcgctcttc
      901 ctcgtcctga tcctcgcggt ggagtgcctg aagcgctcgg ggctgatcga ggtcgtcgtc
      961 gcgaaggtcc tctcccgggt ccgctcggag cgggcgctgg ccgccgccgc gatcgtgacg
     1021 accggcgccg tctcggcgat ggtgacgaac gacgtggcgc tcctcctcgt cgtccccttc
     1081 acgctcgcgt tcgagaaggt cgcccccgag ctcgacccgg tccgcgtcgt cgtcctcgag
     1141 atccaggcgg cgaacctcct cggctgcctg acgccgaccg ggaaccccca gaacctgctc
     1201 ctcttctcga ggggcggctt cacgaccggg tcgttcttcg ccgcccagct tccgtgggtg
     1261 atcgggatgg ccgccgccct gctcgcgctc gtcccgatcc tggtgccgag gcgggcgctc
     1321 cccgcaccgc cgccggctcc gagggccgtc gagcctcgcc tcgccacggc gggcctcttc
     1381 ctcctctcgc tccagctcct cgcgatctcc caggcgatcc cgcgcctcgc ccctctcctc
     1441 gccgccctcc ccgccgcggc gctcctcggg aaagggctcc tgaagaccga cttcacgctg
     1501 ctcggcgtct tctcggctct cttcgtcggc gtggaggggc ttcgccgctc cgcgctcttc
     1561 gagacgctcg acccggtcgc gctcttcggc gccactccgg ggggcttcgt cctctcgggc
     1621 gcgctcctct cgcaggccgt ctcgaacgtc cccgccgcga tcctgctcgc cccgtccgcg
     1681 gcggcggccg gcggcggagc gctcttcacg gcgctcctct acggggtgaa cgccggaggc
     1741 tgcggcacgc cgatcgcgtc cctcgcgaac ctgatcggcg ccgacctcta cctccgcggc
     1801 cgcgcccacc gcgccccgtt ctggcgcctc ttcctcgccg tctccttcgc gctcctcgcc
     1861 gtcgcggtcg cgctctcgct cgccctggcg tgaggggagg gcacccgggg tcaggtcccc
     1921 cctcgggtcc tattcccagc ggagggcgtc gatcgggtcg aggcgggcgg ccttgagggc
     1981 ggggtagagg ccgaagaaga ggccgatgga gatgctgacc ccgaggccga cgaggatgct
     2041 ccagaggggg gtcgcggtcc ggaggggcga gacggcgccg acgacgaggg ccgtcaggac
     2101 gccgagcgcg acgccgaccg cgccgccgag cccggtgagc gtcatcgcct ccgtcaggaa
     2161 ctggaggctg atgtcgcgcc ggagtgcgcc gaccgacttg cgcaggccga tctcccgcgt
     2221 ccgctccgtc acggagacga gcatgatgtt catgacgccg acgcccccga cgaggagcgc
     2281 gatcgccgcg atgaagacca tgacgcccga gatcgcgttc gtcacggcgg tcatctgcgc
     2341 gatgagcttc tccgcggtga agatcgcgaa gtcgttcggc ttgtcgaacg gcaccttccg
     2401 ccgcgcgcgg aggatgttcg tcccctcctc gatcgcctgc tggatcaggt cgggcgactt
     2461 cgggatcgtg gcgatgtgga gcgtgtcgcc gcgggagttc ttgatctgcg ggaagaagac
     2521 gtcgaacgtc gtgatcggga tgatgatgat gttgtccggg ctgtccccca tctgggagcc
     2581 cttcttctcg agcagcccga cgacctggac ccggcggccg ttgatcgtga tcggctgccc
     2641 gagcgggtcc ttcccctcga agagcgactt cacgacgtcg gccccgatga ccgcgacgtc
     2701 ggtggcgtgg cgcacgtcga cgtccgtgat gttccggccg tccgcgagcg cccagttgtt
     2761 gcagacgagg tagttctggt tcccgccggt gaccgtcggc gagttggcct ccttgccgtt
     2821 ccccttcacc ttcgagccgc cccagaggta gcgctccggg gagaccgccg cgatcgacgg
     2881 tgcgagccgg gcgagcgcct cggcgtcctc gatcgtcagg tccttccggt tccgctcctc
     2941 ctcgggcggg cggtgcccgc cgccgaagaa gcgcggctcg aacttctgga actggacgag
     3001 cgtcgtcccg aaggaggaga acgcctccat cgtcgatcgg ttgaagccgg tgacgagggc
     3061 gaccatcgcg atgacggtcc agacgccgat gacgatcccg acgatcgtca ggaaggcgcg
     3121 gagcttgtgg gcgcgcaggg cgatcgaggc gaagcggagg ttctcgccga aagtccggcg
     3181 gagggcgtcg aagatgcgct cactcataac ggagggcctc gatcggcgga aggcgcgagg
     3241 ccttgaccgc cgggaagacg ccggcgacga ggccggtgag cgtggcgagg aggagcccgg
     3301 ccgtgacgag cgtcggggag acgagggtcg ggaggggggt gaaggcgttg atgagcgacg
     3361 agatcaccca gccgaggagg acgccgacgg ctccgccgcc gagagcgagg agggtcgctt
     3421 cgaggaggaa ctgcctccgg atgtcgcgct tcctcgcgcc gatcgcgagg cggatgccga
     3481 tctccttcgt ccgctccacg accgagacga gcatgatgtt cgcgatgacg atcgccccga
     3541 cgacgagcga gatgctgctg atgaaggcga ggagggcgaa ggccccggcc gagatcgagc
     3601 gccagacgac ctggatcgcc tccgccgtga cgaaggagac cgggtcgggg gccgaagcgg
     3661 cggtcttgcg gagcgcccgg aagatcgccc gcgcctcgtc catcgaggcg ggtatcccct
     3721 cgacgccgcc gcgcgccttc acgtagatgc tgatgttcct ccgggtcccc caggccttcg
     3781 cgagcgcctc tcgcgggacg tagacgacgt tgtcctggct ctgcccgaag acggagccct
     3841 gcttgacgag gaggccgatg acgcggaacg ggatcccgtc gatcgagatc gtccgaccga
     3901 ccgggtcgag gcgcggccag agctcgtcgc ggacctcggt gccgatcacc gcggcgaagg
     3961 agcgccgctc gttttccgac tccgtgaaga agcggccgag gtcgacgtcg aggttcgaga
     4021 gctcggcgac gttcgccgtg acgcccgcga tctggacgtc ggagaggcgc cggttccccg
     4081 accggacgga acgcgtcgtc ccggagcggg ccccgaccgc gacggcgtgc ttcgcgagcc
     4141 tgcggtaggc ctcgaggtcg ctcttctgga tccgcttccg cttcagggcg tcgaggaact
     4201 cctcgcgcga ggtgatgatc ccgaatcgct cgatcgtgta gacatccggg gagagggaga
     4261 agagcttcgt cgcgacgtag ttgttgagcc ccgagatgac cgagacgacc gcgatgacgg
     4321 tcatcacccc gatgatgacg ccgaggagcg tcaggaacga gcgcagcttg tgggcgctga
     4381 tggcccgcca ggcgacgcgg aagagctccc cgttcagcat cgagggccta cgacttcttc
     4441 tcgtcccgcg ggacgccgct gttgtcgacc ttcacccggt ccccgatccg gagggcgcgg
     4501 agggcgcgga acggtccgga gacgatctcc tccccttcct tcagcccctc gacgacctcg
     4561 atctggagct cggccgtgat gccggtcttc accttccgga annnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn ncgcctcgaa gttcttccgc tcgcggtcgt agtcccgctt
     4741 cgcctgctcc accgtcgccc ggtccgactc gcgctgggcg aagagcgcct cgaggttcgc
     4801 ctgctgggcc ttcgtcgtcg cgtcgtactg ggccttgtcg atctggagga ggaagtcccc
     4861 cttcttgacg acgtcccctt cctcgacggc caggttgatg atctggccca tgacctgaga
     4921 ggagatctcg accttcgtct tcgcctggac ccggccgctg cagctgacgg tcgagacgag
     4981 gtccgccttc ccgaccttcg ccgtcgtcac ccgggtcagg ttccggtcct tgccggccac
     5041 ggaggcgacg atgacgagga cgacgacgag ggcggcgacc gccccgagga tccatttcag
     5101 ccgggagctc atctctctcg cgttcctttc aagcggtgcc gtgggccggc gccaaacgag
     5161 ggatacgaga gaagggggcc gggagtttcc ctcttcgaag ggtccgaggc cggaaatcga
     5221 agggagcgag ccgtttgccc gcccgcaccg gtcagccgga gacgacccgg ttccgcccct
     5281 cgtccttggc ccggtagagg gcggcgtcgg cccgccggag aagctcggcg gccgccgggg
     5341 ggtgagggag gctttcggtg gaggcgatcc cgaccgagac ggtcacgggc acgggcacgc
     5401 tcaccccgag cggacgccat tccgtggccc gccggatccg ttccgcgacg agcgccgcgg
     5461 ggacgagggc ggtcgtcggg aggaggatcg cgaactcctc gccccccagg cgggcggcga
     5521 ggtcgcctct ccggcagccc cgcttcagga ccgccgcgaa ggcggcgagg acctcgtccc
     5581 ccacggcgtg cccgtgggtg tcgttcacct gcttgaagtg gtcgaggtcg aacgtgagga
     5641 ggctcgccgg ctggttgtgc cgcacgatcc gcgagagctc cgaggcgagc gcctcgccga
     5701 aggcccggcg gttcgacagg ccggtgaggg ggtcggtcag ggcgagcttc tcgaggatct
     5761 cgttcttcga agccaggtcc gagagggcgc gctggaggcg gagcccgacc ttcacccgcg
     5821 ccttcagctc gaccggctcg accggcttga cgaggtagtc gtcggcgccg gtctcgaggc
     5881 cggtcacctt cgcctccgtc tccccctgcg tcgacatcat gatgacgtgg gtgaagcggg
     5941 tctcttcggt ccccttcagg attcggcaga gcgtgaggcc gtccatgttc ggcatctgcc
     6001 agtcggtgac gacgacgtcg gggcggtgca ggcggacctt tccgagcgcc tcctgcccgt
     6061 ccccggcgct ttcgacctcg tagtccgccg ccacgagcac ctgctcgagg acccgcctcg
     6121 agagggcgtc gtcgtcgacg atcaggatgc gggagcggat ctgggccatc aggggggtcc
     6181 cgaaggcggg accgcggatt ctcccacacg agtcagagtg gcggaggagg agggattcga
     6241 acccccgggg cctcgcggcc caacggtttt caagaccgcc gccttaaacc actcggccac
     6301 tcctccgtcg aaggagctgg cggacagggg gggattcgaa cccccggtag gctttgaagg
     6361 cctacgacgg tttagcaaac cgttggttta agccactcac ccacctgtcc gcttcccgcg
     6421 gggggcggag tttagcagag gctccgcgac gggcggccgg accgcgccgg gcgtgccggg
     6481 gggaggaacg ctcagccctt ccggatctcg gcgagcccgc cacagtaggg gcggagagcc
     6541 tccgggatcg ccaccgagcc gtcctcccgc tggccgttct cgaggatcgc gacgagcgtc
     6601 cgcccgaccg cgagcccgga gccgttgagg gtgtggacga actcgttccg gctcttcccc
     6661 tcggcggtcc gcttcacccg gatcgccgcc cggcgggcct ggaaggcgcc gaagttcgag
     6721 cacgaggaga tctcccggta ggcgtcctgc cccgggagcc agacctcgag gtcgtacgtc
     6781 ttcgccgagg agaagcccat gtcgccgcgg cagaggacga ccctccggta cggcagcccg
     6841 agccgctcga ggaccgtctc ggcgtcgcgg gtcagcctct catgcgcctc cgccgaccct
     6901 tcggccgtcg tgaacttgac gagctcgacc t
//
LOCUS       DAOTEZ010000218         6860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_7898_length_6860_cov_5.938575, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000218 DAOTEZ010000000
VERSION     DAOTEZ010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6860
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7898_length_6860_cov_5.938575"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="P5164_12070"
     CDS             complement(<1..713)
                     /locus_tag="P5164_12070"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRY44668.1"
                     /translation="MVVPGEAAAFAVGRSAAWCRLVERAARVAPLGVTVLIRGETGSG
                     KDVVARLIVALGDREGAPFVAVNCAALPDTLVESELFGHARGSFSGATEARRGLFEEA
                     HRGTLFLDEIGALPLPAQAKLLRVLEEGRVRRVGENRVTEVRVRVLAATSLDMEAAIG
                     RREFREDLYYRLSALELVVPPLRERPEDIPLLAEHFLRLLREQGGPERTLSAEALDLL
                     TGYPFPGNVRELKHAVEQAA"
     gene            762..1703
                     /locus_tag="P5164_12075"
     CDS             762..1703
                     /locus_tag="P5164_12075"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HRY44669.1"
                     /translation="MATPRLILLEPDGARAESLVLTLEELRMPVTVAATVDEAAAAAD
                     SGPAVVLTSGVGRGGAARLRELREDPRTASLGVLAVAARGAGSLAALRAGADDVVPWP
                     SPAPLVRARLRGLLALARSREESHRTVAAFAGLLEAFEAREPHRIEHSARVGRLAAGM
                     GRRMGLSEAEVEKIRVGGVLHDIGTVAIPDSILYKRDRLTDGELAVVKSHPVIGFSLI
                     RDVPALEPYGPFVLRHHERIDGSGYPDGLRGSEIPLPVQLVALADAFDAMTSSRPYRA
                     VRDDGQTLTVLEAEARRGQWDPSLLPLLVAEAESPAG"
     gene            1852..3342
                     /gene="ahcY"
                     /locus_tag="P5164_12080"
     CDS             1852..3342
                     /gene="ahcY"
                     /locus_tag="P5164_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRY44670.1"
                     /translation="MSTVLDPKVAPASSARPKFKVADLALAELGRKEIRLAEQEMPGL
                     MALRERHRGEKPLAGERVMGSLHMTVQTAVLIETLVELGADVRWVSCNIFSTQDHAAA
                     AVVVGRPETGGTPGSPKGTPVFAWKGETLEEYWWCTREAIEWPDGSGPTLIVDDGGDA
                     TLLVHKGLEFETAGKVPAFDPKNDPEEWGVILELIRTELAKDGKAFSRLAKAIRGVSE
                     ETTTGVHRLYQMEKDGTLLFPAINVNDSVTKSKFDNVYGCRHSVVDGLNRATDVMLGG
                     KVAVVLGFGEVGKGCAEALRGQGCRVVVTEIDPICALQAAMQGYEVKTLEDVVGTADI
                     FITATGNLDVVTAEHMAKMKDKAIVGNIGHFDNEIDMAGLKKVPGVTRINIKPQYDEF
                     RFADGHSVMILAEGRLMNLGCATGHPSFVMSSSFTNQVMAQIALAKNQGEYGKKVYVL
                     PKKLDEEVARLHLAHLGVKLTQLTPKQAGYLGVPAEGPYKPDHYRY"
     gene            complement(3427..4911)
                     /locus_tag="P5164_12085"
     CDS             complement(3427..4911)
                     /locus_tag="P5164_12085"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HRY44671.1"
                     /translation="MRVGGALLLLGLPFWAGCLTAPRATVLYVTDAHEIAPVVDRHGE
                     RGGVARLKTVVDRLGRGGPDALLVFGGDLAGGTLFGGVFRGEPMVDALGRVGVGLATF
                     GQHEFDFGPAHAERLVRRSTFPWITSNLVDEAGRPFAALPTGRLVRVGGLAVGFLGLT
                     DDLGTTTPDRAVRQTELVGAAAREAAALRRAGAEAIVVLTQAGFEVNRRILAEVPEVD
                     AVLTEEESESVSVVRWIGGRPIAAPNGNLGSVVELTLERARGHLSSRVAVHPVDASVV
                     PDPELSEMETELMVRLGKELAAPAGALARLLDETGSSVRETPLGSLVADAFRAGTGAD
                     VALVPGSSLRAPLPAGPLSMRNVVAVLPFGNRVATVELVGADLRAVLERSLAAVGGRT
                     GSLLQLSGMTLEADLGAPAGRRLLAVRIADVPLREDARYRVAVSSFLAAGGDGQAELA
                     AGRRLEGVEPLDADLLAEHLRRLSRGGPAEPPPLGRMALTPPRG"
     gene            complement(4952..>5668)
                     /locus_tag="P5164_12090"
     CDS             complement(4952..>5668)
                     /locus_tag="P5164_12090"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003566160.1"
                     /note="reversible synthesis of carbamate and ATP from
                     carbamoyl phosphate and ADP; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HRY44672.1"
                     /translation="GAQTEGMIGYVIEQEMGNLLPFERPFATILSMTEVDPADPAFRN
                     PTKPIGPVYTKEDADRMAAEKGWTFKPDGNGWRRVVPSPHPKRIFEIRPVKWLLEKGT
                     VVIFAGGGGIPTMYGPDRTLKGIEAVIDKDLASSLLAREVGADAFVMATDADAVYVGW
                     GTPEARALKTVSIAELKKYSFPAGSMGPKVQAACEFAEATGKAACIGALEDTARMLRG
                     EAGTTVVRDDVEPEYWPRKG"
     assembly_gap    5669..5768
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5771..6772)
                     /gene="argF"
                     /locus_tag="P5164_12095"
     CDS             complement(5771..6772)
                     /gene="argF"
                     /locus_tag="P5164_12095"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014579103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRY44673.1"
                     /translation="MSFNLKNRHFLTIHDFTPKEILYLLDLARDLKRAKYAGTEVKRL
                     GGKNVALIFEKDSTRTRCAFEVACYDQGANVTYLGPSGSQIGKKETMKDTARVLGRMY
                     DAIEYRGYGQAIVEELAKWAGVPVYNGLTDEFHPTQILADLLTMREHSDKPLGQISYA
                     YLGDARNNMGNTLMLGGALMGMDVRLGAPKHLWPVEELVAKANELAAASGGKVTLTED
                     PKAAVAGVDFVHTDVWVSMGEPDSVWAERIKLLLPYQVNAALMAATGNPKAKFLHCLP
                     SFHNRDTKIGEEIFKKFGIDAMEATDDVFESPASVVFDQAENRLHTIKAVLVATLAG"
BASE COUNT          969 a         2518 c         2340 g          933 t
ORIGIN      
        1 gccgcctgct cgacggcgtg cttcagctcc ctcacgttcc cgggaaacgg gtagcccgtc
       61 agcaggtcga gggcctccgc cgagagcgtc cgttccggac cgccctgctc gcgaagcaga
      121 cggaggaagt gctcggccag gagcgggatg tcctcggggc gctcccggag cggcgggacg
      181 acgagctcca gggccgagag cctgtagtag aggtcctcgc ggaactcccg ccgcccgatc
      241 gcggcctcca tgtcgaggct cgtcgcggcc aggacgcgga cccggacctc cgtgacccgg
      301 ttctcgccca cccggcggac gcgcccttcc tcgaggacgc gcagcagctt cgcctgcgcg
      361 gggagcggga gagccccgat ctcgtccagg aagagggtcc cgcggtgcgc ttcctcgaag
      421 agcccccggc gcgcctcggt ggcgccgctg aacgacccgc gggcgtgccc gaagagctcg
      481 ctctcgacga gggtgtcggg gagcgccgcg cagttgaccg cgacgaaggg cgccccctcg
      541 cggtccccga gggcgacgat caggcgggcg acgacgtcct tccccgaccc cgtctcgccg
      601 cggatcagca cagtgacacc gagcggggcg acgcgggccg cccgctcgac gagccgacac
      661 caggctgcgg accggccgac ggcgaacgcc gccgcttcac cggggacgac cattcgagcg
      721 agtctagcct ctcgacgagc gattcgcgct atcgtccgcg cgtggcgacg ccccgtctca
      781 tcctgctcga gcccgatggg gcgcgggcgg agagcctcgt cctcacgctc gaagagctgc
      841 ggatgcccgt gaccgtcgca gcgaccgtcg acgaggccgc cgccgccgcc gactcggggc
      901 ccgccgtcgt cctgaccagc ggcgtcggac ggggcggagc ggcccggctt cgggagctcc
      961 gggaagaccc ccgcaccgcc tctctgggcg tcctcgcggt cgcggcgcgg ggggccggga
     1021 gcctcgccgc gctccgggcc ggcgccgacg acgtcgtccc gtggccctcg ccggcgccgc
     1081 tcgtgagggc ccggctccgg gggctcctcg cgctcgcgcg ctcccgcgag gagtcccacc
     1141 gcacggtcgc cgccttcgcc ggcctcctcg aggcgttcga ggcacgcgag ccgcatcgga
     1201 tcgagcactc cgcccgcgtc ggccggctcg cggccgggat gggccgacgc atgggccttt
     1261 ccgaggccga ggtcgagaag atccgcgtcg gcggggtcct ccacgacatc gggacggtcg
     1321 cgatccccga cagcatcctc tacaagcgcg atcggctcac cgacggcgag ctggccgtcg
     1381 tcaagtccca cccggtcatc gggttcagcc tgatccgcga cgtcccggcc ctggagccgt
     1441 acggcccctt cgtcctccgc caccacgaga ggatcgacgg gtcaggctac ccggacggac
     1501 tccggggctc cgagatcccc ctgccggtcc agctcgtcgc cctcgccgac gcgttcgacg
     1561 ccatgacctc ctcgcgtccc taccgcgccg tccgggacga cgggcagacg ctcaccgtcc
     1621 tcgaggcgga ggcgcgccgc ggccagtggg acccctcgct cctcccgctc ctcgtcgcgg
     1681 aagccgagtc cccggcgggc tgacgccccg ttttgacaag gggcggcccc cgttgggaga
     1741 ctccccgccc ggacactgcc tcctgccatc cggcgagagg caggctccgt accacgatcg
     1801 gccaggagca cgccgtcccc cgacgacggg gcgagccggc caaggagaag catgagcacc
     1861 gtcctcgacc cgaaggtcgc ccccgcgtcc tccgcccgac cgaagttcaa ggtcgccgac
     1921 ctcgccctcg ccgagctcgg ccgcaaggag atccgcctcg ccgagcagga gatgcccggc
     1981 ctcatggccc tgcgcgagcg tcaccgcggc gagaagccgc tcgccggcga gcgcgtcatg
     2041 ggaagcctcc acatgaccgt ccagacggcc gtcctgatcg agactctcgt cgagctcggc
     2101 gcggacgtcc gctgggtctc ctgcaacatc ttctcgacgc aggaccacgc cgccgcggcg
     2161 gtcgtcgtcg gccggcccga gaccggcgga acgcccggga gcccgaaggg gactccggtc
     2221 ttcgcctgga agggcgagac gctcgaggag tactggtggt gcacgcgcga ggcgatcgag
     2281 tggcccgacg gcagcggccc cacgctcatc gtcgacgacg gcggcgatgc cacgctcctc
     2341 gtccacaagg ggctcgagtt cgagacggcc gggaaggtcc ccgcgttcga cccgaagaac
     2401 gacccggagg agtggggcgt catcctcgag ctgatccgta cggagctcgc gaaggacggg
     2461 aaggccttct cccgcctcgc gaaggcgatc cgcggcgtct ccgaggagac gacgacgggc
     2521 gtccaccgcc tctatcagat ggaaaaggac ggcacgctcc tcttccccgc catcaacgtg
     2581 aacgactccg tgacgaagtc gaagttcgac aacgtctacg gctgccgcca ctccgtcgtc
     2641 gacggcctga accgggcgac ggacgtcatg ctcggcggca aggtcgccgt cgtcctcggt
     2701 ttcggcgagg tcggcaaggg gtgcgccgag gcgctccgcg ggcagggctg ccgcgtcgtc
     2761 gtcaccgaga tcgacccgat ctgcgcgctc caggccgcga tgcagggcta cgaggtgaag
     2821 acgctcgagg acgtcgtcgg gacggcggac atcttcatca ccgcgaccgg gaacctcgac
     2881 gtcgtcaccg ccgagcacat ggcgaagatg aaggacaagg cgatcgtcgg gaacatcggc
     2941 cacttcgaca acgagatcga catggccgga ctgaagaagg tccccggcgt cacgcggatc
     3001 aacatcaagc cgcagtacga cgagttccgg ttcgccgacg gccactcggt catgatcctc
     3061 gccgaagggc gcctcatgaa cctcggctgc gccacgggcc acccgagctt cgtcatgtcg
     3121 tcctcgttca cgaaccaggt catggcgcag atcgccctcg cgaagaacca gggcgagtac
     3181 gggaagaagg tctacgtcct tccgaagaag ctcgacgagg aggtcgcgcg gctccacctc
     3241 gcccacctcg gcgtgaagct gactcagctc acgccgaagc aggccgggta cctcggcgtc
     3301 ccggcggaag gtccgtacaa gcccgaccac taccgctact gaccgccgcg gggcgtcctc
     3361 gttcgaccga aggcccgggg cgacccgggc cttcgccgtt tcgccccctc gccggaggtt
     3421 tctccgtcat cccctggggg gcgtgagcgc cattcgcccg agcggcggcg gctccgccgg
     3481 gccgccccgg gacaggcgcc ggaggtgctc cgcgagaagg tcggcgtcga gcggctcgac
     3541 cccctcgagc cgccgcccgg cggccagctc ggcctggccg tcgccgccgg cggcgaggaa
     3601 gctcgacacc gcgacgcggt acctcgcgtc ctcgcggagc ggaacgtcgg cgatccggac
     3661 cgcaaggagg cggcgccccg ccggcgcgcc gaggtcggcc tcgagggtca tcccggagag
     3721 ctgcagcagg ctgcccgtcc ttcctccgac cgccgcgagg ctcctctcga gcaccgcgcg
     3781 aaggtccgcc ccgacgagct ccaccgtcgc cacgcggttc ccgaacggca ggacggccac
     3841 gacgttccgc atcgagagcg ggccggccgg aagcggcgca cggaggctcg agccggggac
     3901 gagcgcgacg tccgcgcccg tccccgcgcg gaaggcgtcg gccacgaggc tgccgagcgg
     3961 cgtctcccgc accgagctcc cggtctcgtc gagcaggcgg gcgagcgctc cggccggggc
     4021 cgcgagctcc ttccccagcc gcaccatgag ctccgtctcc atctccgaga gctccgggtc
     4081 ggggacgacg gaggcgtcga ccgggtggac cgcgactcgg gaagagaggt gcccgcgcgc
     4141 tctctcgagc gtcagctcga cgaccgaccc gaggttcccg ttcggtgcag cgatcgggcg
     4201 tcccccgatc cagcggacga ccgagaccga ctcggactcc tcctcggtca ggacggcgtc
     4261 gacctccggg acctccgcga ggatccgtcg gttcacctcg aagccggcct gcgtcagcac
     4321 gacgatcgcc tcggcccctg cccgcctcag agcggccgcc tcgcgcgccg cagcccccac
     4381 gagctcggtc tgccggaccg cccggtcggg tgtcgtcgtg ccgaggtcgt ccgtcagccc
     4441 gaggaagccg acggcgagcc ccccgacccg gacgagccgg cccgtcggga gcgccgcgaa
     4501 cggacgcccg gcctcgtcga cgaggttcga cgtgatccag gggaaggtgg agcgccggac
     4561 caggcgctcg gcgtgggccg gcccgaagtc gaactcgtgc tggccgaacg tcgcgaggcc
     4621 gactccgacg cgaccgaggg cgtcgaccat cggctccccg cggaagacgc cgccgaagag
     4681 cgtgccgccc gcgaggtccc cgccgaagac gaggagcgcg tccggccctc ctctcccgag
     4741 gcggtcgacg accgtcttca ggcgtgccac gccccccctc tcgccgtgcc ggtcgacgac
     4801 cggcgcgatc tcgtgcgcgt ccgtgacgta gaggacggtc gcgcgcggag cggtgagaca
     4861 gccggcccag aacggcagcc cgagaagaag gagggccccg ccgaccctca cgggaggcgg
     4921 ggccccggac cgccgcaggc ggacatcggc ctcagccctt ccgcggccag tactccggct
     4981 cgacgtcgtc cctcacgacc gtcgttccgg cctctccccg gagcatccgg gccgtgtcct
     5041 cgagcgcccc gatgcaggcc gcctttccgg tcgcctcggc gaactcgcac gcggcctgga
     5101 ccttcggccc catcgacccg gccgggaagg agtacttctt cagctcggcg atcgagaccg
     5161 tcttcagcgc ccgcgcctcg ggagtccccc agcccacgta gacggcgtcg gcgtccgtcg
     5221 ccatgacgaa cgcgtccgcg ccgacctcgc gcgccaggag cgaggaggcg aggtccttgt
     5281 cgatgaccgc ctcgatcccc ttcagagtgc gatccggccc gtacatcgtc gggatgcccc
     5341 cgccgccggc gaagatcacg accgtcccct tctcgaggag ccacttcacc ggccggatct
     5401 cgaagatccg cttcgggtga ggcgacggga cgaccctccg ccagccgttg ccgtccggct
     5461 tgaaggtcca ccccttctcg gccgccatcc ggtcggcgtc ttccttcgtg tagaccggcc
     5521 cgatcggctt cgtggggttc cggaacgccg ggtcggcggg gtcgacctcg gtcatcgaga
     5581 ggatcgtcgc gaaggggcgc tcgaacggga ggaggttccc catctcctgc tcgatgacgt
     5641 agccgatcat cccttccgtc tgggcgccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnntc ctacccggcc agcgtcgcga cgaggacggc cttgatcgtg tggagacggt
     5821 tctcggcctg gtcgaagacg acggacgccg gagactcgaa gacgtcgtcg gtcgcttcca
     5881 tcgcgtcgat gccgaacttc ttgaagatct cctcgccgat cttcgtgtcg cggttgtgga
     5941 acgaggggag gcagtggagg aacttcgcct tcgggttccc cgtcgccgcc atgagcgccg
     6001 cgttcacctg gtacgggagg aggagcttga tccgctcggc ccagaccgag tcgggctcgc
     6061 ccatcgagac ccagacgtcg gtgtggacga agtcgacgcc ggcgacggcc gccttcgggt
     6121 cctcggtgag cgtcaccttc ccgccggagg ccgcagccag ctcgttcgcc ttcgcgacga
     6181 gctcctcgac cggccagagg tgcttcggcg ctccgaggcg gacgtccatc cccatcagcg
     6241 cgccgccgag catgagggtg ttccccatgt tgttccgggc atcgccgaga taggcgtagg
     6301 agatctgccc gagcggcttg tcgctgtgct cgcgcatcgt gaggaggtcg gccaggatct
     6361 gggtcgggtg gaactcgtcg gtgaggccgt tgtagaccgg gacgccggcc cacttcgcga
     6421 gctcctcgac gatggcctgc ccgtagccgc ggtactcgat ggcgtcgtac atccgcccga
     6481 ggacccgcgc cgtgtccttc atcgtctcct tcttcccgat ctgggagccg gaggggccga
     6541 ggtaggtgac gttcgccccc tggtcgtagc aggcgacctc gaaggcgcag cgggtgcggg
     6601 tcgagtcctt ctcgaagatc agcgcgacgt tcttcccgcc gaggcgcttc acctcggtcc
     6661 ccgcgtactt ggcccgcttc aggtcgcgcg ccaggtcgag gaggtacagg atctccttcg
     6721 gggtgaagtc gtggatcgtc aggaagtggc ggttcttgag gttgaacgac atgggtcctc
     6781 cggcgacggc tttcccctgg cggcggcggg accgctccgg gcacctcccg cgaggctcct
     6841 tccggacgtc cgtcgagcgc
//
LOCUS       DAOTEZ010000219         6824 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_7956_length_6824_cov_6.903088, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000219 DAOTEZ010000000
VERSION     DAOTEZ010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6824
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7956_length_6824_cov_6.903088"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /gene="rpsC"
                     /locus_tag="P5164_12100"
     CDS             <1..315
                     /gene="rpsC"
                     /locus_tag="P5164_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRY44674.1"
                     /translation="QLVSESIAMQLERRVAFRRAMKKGMESAFRFGAQGIKIRCSGRL
                     NGAEIARSEWYQEGRLPLHTLKADIDYGFAEARTTYGKIGVKCWIYKGDLHRAKSRRK
                     VA"
     gene            328..753
                     /gene="rplP"
                     /locus_tag="P5164_12105"
     CDS             328..753
                     /gene="rplP"
                     /locus_tag="P5164_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRY44675.1"
                     /translation="MLMPSKVKYRKQQKGRRRGTALRGGTLSFGEFGLQALEAGWLTA
                     RQIEAGRVAIQRHVKRGGKLWIRVFPDKPITKKPLETRMGKGKGAPEAWVSVIKPARI
                     LFEMEGVEEKIARDAFRLAADKIGIKTRFISRAFEEAVR"
     gene            750..944
                     /gene="rpmC"
                     /locus_tag="P5164_12110"
     CDS             750..944
                     /gene="rpmC"
                     /locus_tag="P5164_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRY44676.1"
                     /translation="MKGTTVETMKGWSADERTVKLREWTQALFNLRLQKATGQLENPM
                     KIRQLRRDIARLKTAERQAR"
     gene            941..1234
                     /gene="rpsQ"
                     /locus_tag="P5164_12115"
     CDS             941..1234
                     /gene="rpsQ"
                     /locus_tag="P5164_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRY44677.1"
                     /translation="MTTETTTAAAKPAARKSRKVGTVVSNKMEKTVVVEVTRLVKHGR
                     YGKFQKKSARFLADDRVHACIPGDVVEIEETRPLSARKRWRVRAVVTAARREA"
     gene            1247..1621
                     /gene="rplN"
                     /locus_tag="P5164_12120"
     CDS             1247..1621
                     /gene="rplN"
                     /locus_tag="P5164_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRY44678.1"
                     /translation="MIQMGTILEVADNSGAKRLSCIRLKGGSVGQYGRLGDVITCAVR
                     ESAPDAPDAVKKGKVVKAVIVRTRREQRRRDGSYIRFDNNAAVLINNDKEPIGTRVFG
                     PVARELREKKYMKIISLAPEVI"
     gene            1632..2000
                     /gene="rplX"
                     /locus_tag="P5164_12125"
     CDS             1632..2000
                     /gene="rplX"
                     /locus_tag="P5164_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRY44679.1"
                     /translation="MKKKQPEYTGKTRVRKGDEVVVLAGKDRGAHGKVLRVVPDKGRV
                     VVEGVNLIKRATRPNPQKNIKGGIVEREAAIAISNVMLRDPETGKPTRVGVRVEGGER
                     VRYARRSKATISSVTKKTGA"
     gene            2004..2546
                     /gene="rplE"
                     /locus_tag="P5164_12130"
     CDS             2004..2546
                     /gene="rplE"
                     /locus_tag="P5164_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRY44680.1"
                     /translation="MSSRLIEKYRKDVVPVLKKEFGIDNPMAVPKIEKVVLNMGVGEA
                     ITNVKIVEAAAEELTKIAGQRAVITRARKSIATFKLREGMPIGCRVTLRGERMYEFLD
                     RLLNIALPRVRDFRGVPTRAFDGRGNYTLGIRDHLVFPEIDPGKVDKSKGLNVTIVTT
                     AGSDDRARVLLRELGMPFGR"
     gene            2566..2751
                     /locus_tag="P5164_12135"
     CDS             2566..2751
                     /locus_tag="P5164_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009110294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HRY44681.1"
                     /translation="MARRSSIVKTARKPKFAIRAHNRCKICGRPRSYIRKFGTCRICF
                     RNLALGGYVPGVTKASW"
     gene            2762..3163
                     /gene="rpsH"
                     /locus_tag="P5164_12140"
     CDS             2762..3163
                     /gene="rpsH"
                     /locus_tag="P5164_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRY44682.1"
                     /translation="MAALTDPIADLLTRVRNGLTSRHEKIDVPSSRLKIEIARILKDE
                     GYIKNFKVLDDKGAGLLRLYLKYDDAGNPVIHGLTRVSRPGRRLYRGKDELPRVLGGL
                     GIAIVSTSQGLLTGHDARKRGLGGEVVCSVW"
     gene            3180..3737
                     /gene="rplF"
                     /locus_tag="P5164_12145"
     CDS             3180..3737
                     /gene="rplF"
                     /locus_tag="P5164_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRY44683.1"
                     /translation="MSRVGKKAITLPKGVDVKVDAAGVHVKGPKGTVSTPVLEGITVA
                     VKDGRVDLVRASEEKRIRAFHGLNRALVANAVLGVAEGYKRSIDLVGIGYKAEKKGSE
                     VIFTCGYSHTVRFPEPDGVTIDVAPDAKAKDVTHIVVTGVDKQKVGETATRIRGIKPP
                     DPYKLKGFRFVGEVLKKKAGKAAGK"
     gene            3748..4116
                     /gene="rplR"
                     /locus_tag="P5164_12150"
     CDS             3748..4116
                     /gene="rplR"
                     /locus_tag="P5164_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRY44684.1"
                     /translation="MNRAQARVEARDRIHTRIRRKVEGSPERPRLAIRRTLKYIYAQL
                     VDDASGRTLAQASSREDSILGGKKSAASKAAAVSVGALIAERAKEKGIAAVVFDRGGH
                     LYHGNVKALADSAREKGLKF"
     gene            4245..4778
                     /gene="rpsE"
                     /locus_tag="P5164_12155"
     CDS             4245..4778
                     /gene="rpsE"
                     /locus_tag="P5164_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRY44685.1"
                     /translation="MIDHVVHINRVTKVVKGGKNFSFSALVIVGDGKGRVGYGAGKAK
                     EVPTAIKKGIEMAKKTMISVTLKGTTIPHAVTGHYGAGRVFLKPAAEGTGVIAGGAVR
                     AILEAVGVQNILTKSLGTTNPHNVVKATFDALRNLHLAASILKARGRTAGASTVEAVA
                     APAAEAVAAPEIPAETK"
     gene            4916..5119
                     /gene="rpmD"
                     /locus_tag="P5164_12160"
     CDS             4916..5119
                     /gene="rpmD"
                     /locus_tag="P5164_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRY44686.1"
                     /translation="MAAAPEAGKKTVRIRQVKSGICAPVDQKQTLKGLGLRRIRQEVV
                     RPDTPALRGQILKVRHLVEVVGE"
     gene            5138..5593
                     /gene="rplO"
                     /locus_tag="P5164_12165"
     CDS             5138..5593
                     /gene="rplO"
                     /locus_tag="P5164_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRY44687.1"
                     /translation="MAHGLHNLGPRKGATSARKRVGRGPGSGLGKTSGKGHKGQQSRS
                     GYSGQLGREGGQMPLHRRLPKRGFKNIFRKEWNTVPVEALERLFAAGATVDAEALGKA
                     GLLKRKGAEVKILGTGELKKTLTVVAHQFTEGARKKIEAAGGRCEDVPA"
     gene            5590..>6824
                     /gene="secY"
                     /locus_tag="P5164_12170"
     CDS             5590..>6824
                     /gene="secY"
                     /locus_tag="P5164_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRY44688.1"
                     /translation="MIVLESFRNVFAVPDLRKRVLFTFGLLAIYRIGAHIPTPGLDAL
                     ALEEFFKASAGGLLGFLDMFSGGALRRLSVFALGITPYITASIILQLLTVVWPYLEKL
                     SKEGEMGRRKITQYTRYGTVVLSVIQGAGIALWLWGTTTPTGKFLVLRDPTSEAGKIP
                     FVLMAILTLTTGTAFVMWLGEQITERGIGNGISLIIFAGIVVGLPTAVWHSFRQIQRG
                     QMSLFTALLLGVFMLAVVAAIVFVERAQRRIPVQYAKRVVGRRVYGGQNTYLPLRVNT
                     GGVIPIIFAVSIIQFPQYLASAFKSDFMKKLADQLAMGQPLYNLFYALAIIFFCYFYI
                     SIIFNPTDTAENMRKYGGFIPGIRPGKATSDYIDSVLTRITLIGSLYLVVVALIPEFL
                     LMGFKVQSIPGIGPWLDANL"
BASE COUNT         1210 a         2360 c         2337 g          917 t
ORIGIN      
        1 cagctcgtct cggagtccat cgcgatgcag ctcgaacggc gggtcgcgtt ccgccgggcg
       61 atgaagaagg ggatggagtc ggccttccgc ttcggcgccc agggcatcaa gatccgctgc
      121 tccggacgcc tcaacggcgc cgagatcgcg cggtcggagt ggtaccagga ggggcgcctg
      181 ccgctccaca ccctcaaggc cgacatcgac tacggcttcg ccgaggcccg cacgacgtac
      241 gggaagatcg gcgtgaagtg ctggatctac aagggcgacc tccaccgcgc gaagtcgcgc
      301 cggaaagtcg cgtaaggaac tcccgccatg ttgatgccct ccaaggtcaa gtaccgcaag
      361 cagcagaagg ggcgccggcg cggcaccgcg ctgcgcggcg ggacgctctc cttcggcgag
      421 ttcggcctgc aggccctcga ggcggggtgg ctcaccgccc gccagatcga ggccggccgc
      481 gtcgcgatcc agcgccacgt caagcgcggc ggcaagctct ggatccgcgt cttccccgac
      541 aagccgatca ccaagaagcc cctcgagacc cgaatgggga agggcaaggg cgcgccggag
      601 gcctgggtct ccgtcatcaa gcccgcccgg atcctgttcg agatggaagg cgtcgaagag
      661 aagatcgccc gggacgcctt ccggctcgcc gccgacaaga tcggcatcaa gacccggttc
      721 atctcgcgcg ctttcgagga ggccgtgcga tgaaagggac gaccgtcgag acgatgaagg
      781 gctggtccgc cgacgagcgc accgtcaagc tccgcgagtg gacgcaggcg ctcttcaacc
      841 tccgcctcca gaaggccacc ggccagctgg agaacccgat gaagatccgc cagctgcggc
      901 gcgacatcgc gcggctgaag acggccgaga ggcaggcccg atgaccaccg agaccaccac
      961 cgcagcggcg aagcccgcag cccgcaagag ccgcaaggtc ggcacggtcg tctccaacaa
     1021 gatggagaag accgtcgtcg tcgaggtcac ccgcctcgtc aagcacggcc ggtacggcaa
     1081 gttccagaag aagtccgccc gcttcctcgc ggacgaccgc gtccacgcgt gcattcccgg
     1141 ggacgtcgtc gagatcgagg agacgcggcc cctctcggcg cgaaagcgct ggcgcgtgcg
     1201 cgcggtcgtg accgccgcgc gccgggaagc gtaagggagg gtcgccatga tccagatggg
     1261 gacgatcctc gaggtcgcgg acaactccgg cgccaagcgc ctctcctgca tccgcctgaa
     1321 gggcgggtcg gtggggcagt acgggcgcct gggcgacgtc atcacctgcg cggtgaggga
     1381 gtccgccccg gacgcgcccg acgcggtcaa gaaggggaag gtcgtgaagg ccgtcatcgt
     1441 gcgcacgcgg cgcgagcagc gccggcggga cggcagctac atccgcttcg acaacaacgc
     1501 ggccgtcctg atcaacaacg acaaggaacc gatcgggacc cgcgtgttcg ggccggtcgc
     1561 tcgggagctt cgcgagaaga agtacatgaa gatcatctcg ctcgctcccg aggtgatctg
     1621 aggcgaacgc gatgaagaag aagcagcccg agtacacggg gaagaccagg gtccggaagg
     1681 gcgacgaggt cgtcgtcctg gccggcaagg accggggcgc gcacgggaag gtcctgcgcg
     1741 tcgtccccga caagggacgg gtcgtggtgg agggtgtgaa cctcatcaag cgcgccacgc
     1801 gcccgaaccc ccagaagaac atcaagggcg ggatcgtcga gagggaggcg gccatcgcca
     1861 tctcgaacgt catgctccgc gacccggaga ccggcaagcc gacccgcgtc ggcgtccgcg
     1921 tcgagggggg ggagcgcgtg cgctacgcgc gccggagcaa ggcgacgatc tcctccgtga
     1981 ccaagaagac gggagcgtaa gcgatgtcgt cgcggctcat cgaaaagtac cgcaaggacg
     2041 tcgtcccggt cctcaagaag gagttcggga tcgacaaccc gatggcggtc ccgaagatcg
     2101 agaaggtcgt cctgaacatg ggcgtcggag aggcgatcac gaacgtcaag atcgtcgagg
     2161 ccgccgccga ggagctgacc aagatcgccg ggcagcgcgc ggtcatcacg cgcgcccgca
     2221 agtcgatcgc gaccttcaag ctccgcgaag gcatgccgat cggctgccgg gtgaccctgc
     2281 gcggcgagcg gatgtacgag ttcctcgacc gcctcctcaa catcgccctt ccgcgggtgc
     2341 gggacttccg cggcgtgccc acgcgggcct tcgacggccg cgggaactac acgctcggca
     2401 tccgcgacca cctcgtcttc cccgagatcg atccgggcaa ggtggacaag tcgaagggcc
     2461 tcaacgtcac gatcgtgacg acggccggct ccgacgacag ggcgcgcgtc ctgctccgcg
     2521 agctgggcat gccgttcggg cgctaggaac aagggagaac cgtcgatggc tcgtcgctcc
     2581 tcaatcgtca agaccgcgcg gaagccgaag ttcgcgatcc gggcgcacaa ccggtgcaag
     2641 atctgcggcc ggccgcgctc ctacatccgc aagttcggca cctgccgcat ctgcttccgc
     2701 aacctcgcgc tcggcggcta cgtcccgggc gtgaccaagg cgtcgtggtg aggaggggcg
     2761 cgtggccgca ctgaccgacc cgatcgccga cctcctgacc cgcgtccgca acgggctcac
     2821 ctcccgtcac gagaagatcg acgtcccgtc gtcccgcctg aagatcgaga tcgcccgcat
     2881 cctgaaggac gagggctaca tcaagaactt caaggtgctc gacgacaagg gcgccggcct
     2941 cctccgcctc tacctcaagt acgacgacgc cgggaacccc gtgatccacg ggctgacgcg
     3001 cgtctcgcgc cccggccgcc ggctctaccg cgggaaggac gagctcccgc gcgtcctcgg
     3061 cgggctcggc atcgccatcg tctcgacctc gcagggcctc ctcaccggcc acgacgcgcg
     3121 caagcgcggc ctgggcggcg aggtcgtctg ctcggtctgg taggcccgga gggaagacga
     3181 tgtctcgagt gggaaagaag gccatcacac tcccgaaggg cgtcgacgtc aaggtcgacg
     3241 ccgccggggt ccacgtcaag gggcccaagg ggaccgtctc cacgccggtc ctcgagggaa
     3301 tcacggtcgc cgtcaaggac ggccgcgtcg acctcgtccg cgcgagcgaa gagaagcgga
     3361 tccgcgcgtt ccacgggctg aaccgcgcgc tcgtcgcgaa cgccgtcctc ggcgtggccg
     3421 aggggtacaa gcgctccatc gacctcgtcg gcatcggcta caaggccgag aagaaggggt
     3481 ccgaggtcat cttcacgtgc ggctactcgc acacggtccg gttcccggag ccggacggcg
     3541 tgacgatcga cgtggcgccc gacgcgaagg cgaaggacgt gacgcacatc gtcgtgaccg
     3601 gcgtcgacaa gcagaaggtc ggcgagacgg cgacccggat ccgcggcatc aagccgcccg
     3661 atccctacaa gctcaagggc ttccgcttcg tcggcgaggt cctgaagaag aaggccggaa
     3721 aggccgccgg caagtgaggt ctcgaggatg aacagggctc aggcacgggt cgaggcgcgc
     3781 gacaggatcc acacccggat ccgccggaag gtcgagggtt cgccggagcg gccgcggctg
     3841 gcgatccgca ggactctgaa gtacatctac gcccagctcg tggacgacgc gtccggccgg
     3901 acgctcgccc aggcgagctc ccgcgaggac tcgatcctcg gcgggaagaa gagcgccgcg
     3961 agcaaggcgg ccgcggtctc cgtcggcgcg ctcatcgcgg agcgggcgaa ggagaagggc
     4021 atcgccgcgg tcgtcttcga ccggggcggc cacctctacc acggcaacgt caaggctctc
     4081 gccgactcgg cgcgcgagaa ggggttgaag ttttgagcag cacaccctcc cgtccccccc
     4141 gcttcggaaa ccgtccgggc ggcccgggcc gcggccccgg cggtccgaac gccgctccgc
     4201 cccggccgcc gggcgccctc ggcgagggcc gcgcgggcct cgacctgatc gaccacgtgg
     4261 tgcacatcaa ccgcgtcacg aaggtcgtca agggcggcaa gaacttctcg ttctccgcgc
     4321 tcgtcatcgt cggtgacggc aagggacgcg tcggctacgg cgcgggcaag gcgaaggaag
     4381 tccccacggc catcaagaag gggatcgaga tggccaagaa gacgatgatc tccgtgacgc
     4441 tcaaggggac gacgatcccc cacgcggtca cgggccacta cggcgccggc cgcgtcttcc
     4501 tcaagccggc ggccgaaggc accggcgtca tcgccggcgg cgcggtccgg gcgatcctgg
     4561 aggcggtcgg ggtccagaac atcctgacga agtcgctcgg gacgacgaac ccgcacaacg
     4621 tcgtcaaggc gacgttcgac gccctgcgga acctccacct ggccgcgtcg atcctcaagg
     4681 cgcgcggccg gacggccgga gcgtcgaccg tcgaggcggt cgcggcgccg gcggccgagg
     4741 ccgtcgcggc tccggagatc ccggcggaga cgaagtgatg gccgagaaag agaagagctc
     4801 caagaaggac gtcgacgccc tcctgacgga ggtggcgtcg gcggccggcg tgccgaagaa
     4861 gacggcgaag ccggcggcca ccgtcccggc gaaggccgcc ccggcggctc ccgcgatggc
     4921 cgcggcgccg gaggccggga agaagacggt ccggattcgc caggtgaagt ccggcatctg
     4981 cgccccggtc gaccagaagc agaccctcaa gggcctcggc ctccggcgga tccgccagga
     5041 ggtggtccgg cccgacacgc ccgcgctccg cgggcagatc ctcaaggtcc gccacctcgt
     5101 cgaggtcgtg ggcgagtagc gagagggacg agacaccatg gctcacggac tccacaacct
     5161 cggtccgcgc aagggcgcga cgtcggcgcg caagcgcgtc ggccgcggcc cgggctccgg
     5221 cctcggcaag acctcgggca agggccacaa gggccagcag tcgcgcagcg gctactccgg
     5281 ccagctcggc cgcgagggcg gccagatgcc gctccaccgg cgcctcccca agcgaggctt
     5341 caagaacatc ttccgcaagg agtggaacac cgtcccggtc gaggcgctcg agcgcctctt
     5401 cgcggcgggc gccactgtcg acgccgaagc cctcggcaag gccggccttc tgaagcgcaa
     5461 gggcgccgag gtgaagatcc tcgggacggg cgagctgaag aagacgctga ccgtcgtcgc
     5521 tcaccagttc acggaagggg cgcgcaagaa gatcgaagcg gcgggcggcc gctgcgagga
     5581 cgtcccggcg tgatcgtcct cgagtccttc cggaacgtct tcgcggtccc ggacctcagg
     5641 aagagggtcc tcttcacctt cgggctgctg gcgatctacc ggatcggcgc gcacatcccg
     5701 acgcccgggc tcgacgccct ggcgctcgag gagttcttca aggcgagcgc cggcgggctc
     5761 ctcgggttcc tggacatgtt ctcggggggc gctctccgcc gtctctccgt cttcgcgctc
     5821 gggatcacgc cgtacatcac ggcgtcgatc atcctgcagc tcctcaccgt cgtctggccc
     5881 tacctcgaga agctctccaa ggaaggggag atgggccggc gcaagatcac gcagtacacc
     5941 cggtacggca cggtagtcct ctcggtgatc cagggcgcgg gcatcgccct ctggctctgg
     6001 gggacgacga cgccgaccgg caagttcctc gtcctccggg acccgacgtc cgaggccggg
     6061 aagatcccgt tcgtcctgat ggcgatcctc acgctgacga cgggcaccgc cttcgtcatg
     6121 tggctcggcg agcagatcac cgagcgcggg atcgggaacg gcatctcgct catcatcttc
     6181 gccggcatcg tcgtcgggct ccccaccgcg gtctggcact cgttccggca gatccagcgc
     6241 ggccagatgt cgctcttcac ggccctcctc ctcggcgtct tcatgctcgc ggtcgtcgcg
     6301 gcgatcgtct tcgtcgaaag ggcgcagcgg cgcatcccgg tccagtacgc caagcgggtc
     6361 gtcgggcgcc gcgtgtacgg cgggcagaac acctacctgc ccctccgggt gaacacgggc
     6421 ggcgtcatcc cgatcatctt cgccgtctcg atcatccagt tcccgcagta cctcgcctcg
     6481 gcgttcaaga gcgacttcat gaagaagctg gccgaccagc tcgccatggg gcagccgctc
     6541 tacaacctct tctacgcgct ggcgatcatc ttcttctgct acttctacat ctcgatcatc
     6601 ttcaacccga cggacaccgc ggagaacatg cggaagtacg gcgggttcat cccgggcatc
     6661 cgcccgggca aggcgacgtc ggactacatc gacagcgtcc tgacgaggat caccctcatc
     6721 ggctcgctct acctggtcgt cgtcgcgctc atcccggagt tcctgctcat gggcttcaag
     6781 gtccagagca tcccgggaat cggaccgtgg ctcgacgcga acct
//
LOCUS       DAOTEZ010000220         6821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_7966_length_6821_cov_5.694059, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000220 DAOTEZ010000000
VERSION     DAOTEZ010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7966_length_6821_cov_5.694059"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1818)
                     /locus_tag="P5164_12175"
     CDS             complement(<1..1818)
                     /locus_tag="P5164_12175"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY44689.1"
                     /translation="MKILAIRGKNLASLSGEFALDFETEPLKSSGLFAITGPTGAGKS
                     TLLDALCLALFDATPRLDTRGGPEIGRAGDAERLPAYDVRSLMSRGATDAMAQVDFVG
                     RDDHRWRATWTVGRARGKKGGKVQKQNLSLVDLTVDQTAGGTKTETLTAIEDKLGLTF
                     DQFRKSVLLAQGDFADFLKANADERSLLLQRLTGAEIYERISVAAHRKAKEAKDRLRE
                     LEALRASVTVLSAEEREHLEAEAVAAAAEVEAHGAREKVLEELARQAKAAEESEEEAR
                     SRATTSLKAAGEAEAAGKRRESTQNEVVGATETLALATADLEARKPEIEKARELDVRV
                     SGAERALEEAKAAAAGASGKLEEARKALVDREGIERGLREEVGTLEAWLAGHSAAKEV
                     AARWEQADASLGELVRIADERAATTKRHDEAIRALESASRTAAAAGAAVDETRRQLTV
                     ATTARNEADAKLKGIPRAAHRERLDGLASRERNLAGLRDLFERATAAEKDRLQAVRNA
                     AGAEEKAKTARTEETAAAARLGELALLVPEAKRALALSESALSLEEQRAGLVDGQPCS
                     LCGATEHPWAVAGAPVPAAVELQRKRVRELETEERSAVAA"
     gene            complement(1815..3071)
                     /locus_tag="P5164_12180"
     CDS             complement(1815..3071)
                     /locus_tag="P5164_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002618490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D C-terminal
                     domain-containing protein"
                     /protein_id="HRY44690.1"
                     /translation="MRIVHTADWHLGHVLYDRDREAEHRAFLDWLLDLLEKESVDALL
                     VCGDVFEGANPPASALALWYGFLADASRRCRGLQVVVVAGNHDSPARLEAPAPVLAAL
                     SVNVVGFLPRAEAEVDVERLVVPLRERSSGPGGQVAAWVAAVPFLRLGDLPGGEATPE
                     GIVDGVRAIYARALEAARSRRTAGQALLATGHCYVEGMRLSDESERKVLGGNQHPLPV
                     DVFPADVDYVALGHLHFPQQVGGRGNVRYSGSPLPLSLDEDVYPHQVCLVELEGGRCK
                     DVKSVRVPRSVPILRLPGEPAPLAEVLALLEKLPPAPPREAAEPDRRPWLEVRVRLEG
                     SEFGYQGEIAEALEGKDARLVKITPQRAGSGVTLADGSERSLSDLTPEKVFRKLWERG
                     NADPVPAELEAAFHELVDAASQEEAS"
     gene            complement(3327..3539)
                     /locus_tag="P5164_12185"
     CDS             complement(3327..3539)
                     /locus_tag="P5164_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44691.1"
                     /translation="MLGMKGFDVNDPARKYRLRSLPGEFSGLHLVCILDVGMKLVDPS
                     ADTGFDLSREYEEAKALFGATAEGLT"
     gene            complement(3640..4953)
                     /locus_tag="P5164_12190"
     CDS             complement(3640..4953)
                     /locus_tag="P5164_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY44692.1"
                     /translation="MRETLKALILDLQEAPLETGVPRRLAVRPVAGKATILIGVRRCG
                     KSTLALQQVERLLAQGVPREDVLLVNFFDDRLAGLAREGPGLVTEAYYALYPEKKGKE
                     TVHCFFDEIQAVPGWEPFVDRLLRTEKCRVTLTGSSARMLSKEVATQMRGRALSWELF
                     PFSFRELLDARGIEASGTLSTRKRLLVEKVFGEYWETGGFPEVIRLDRSLRVKTHQEY
                     FHAILYRDLVERHDVAHPKALSDLARWLVDHAASLYTVNRLTGYLKSLGHGVPKAAVA
                     SYLEWFEDAYFLYTVSLFDASLARRNTNPKKVYCVDHALVTSVSSGLFVNSGHLLENL
                     VFVALRRAHPEVSYYKTRSGREVDFVVPGRGRPEALVQVCETLFDPETRKREVAALEA
                     AMEELGTKEGTIVTRNESERIVTASGTIAVVPAWRFLLDQPDAAE"
     gene            complement(4975..5328)
                     /locus_tag="P5164_12195"
     CDS             complement(4975..5328)
                     /locus_tag="P5164_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44693.1"
                     /translation="MPGASSARRAGSRHGRIVLVRDGSPSADPAGAFTVKEYRSERVW
                     GDDGNFLHTRIELRTRSRNTVRQPIVLTPEDEGEVRVMAELVEVGGYGRGRKVAPRGT
                     RPHPETFVTGYDHIR"
     gene            complement(6125..6550)
                     /locus_tag="P5164_12200"
     CDS             complement(6125..6550)
                     /locus_tag="P5164_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44694.1"
                     /translation="MKSASKPQLASVEQPFGGTPPRPHCPICGRAVLRVDDDGLTHAD
                     PCRHLAFVFVGEVGDFEYRSPDFTRRTAHRRIHDLDLTRLRTLLVNAGYGNGLLALEI
                     TYGGMACGPVWYTDVYGFDYGVGGEEEGKEGGSEGGGGA"
BASE COUNT          891 a         2544 c         2362 g         1024 t
ORIGIN      
        1 cgccgcgacc gcgctcctct cctcggtctc cagctcgcgc acgcgcttcc gctggagctc
       61 gacggcggcc ggcacgggcg cccccgccac cgcccacgga tgctccgtgg ctccgcagag
      121 cgagcacggc tgcccgtcta cgaggccggc ccgctgctct tccaggctca gggcggactc
      181 agagagggcc agcgcccgct tcgcctccgg gacgagcagc gccagctcgc cgagccgcgc
      241 ggcggcggcc gtctcctcgg tccgcgctgt cttcgccttc tcctcggcgc cggccgcatt
      301 ccgcaccgcc tggagccggt ccttctccgc ggcggtcgcc cgctcgaaga ggtcccgcag
      361 cccggccagg ttcctctcgc gcgaggccag cccgtcgagc ctctcgcgat gggctgcccg
      421 gggaatcccc ttcagcttcg cgtcggcctc gttccgggcg gtcgtggcca cggtcagctg
      481 ccgccgcgtc tcgtccaccg cggcgcccgc ggccgccgca gtccgactcg cggactccag
      541 ggcgcggatg gcttcgtcgt gcctcttcgt cgtcgcggcc cgctcgtcgg cgatccgcac
      601 cagctcgccg aggctcgcgt cggcctgctc ccaccgggcg gccacctcct tcgccgcgga
      661 gtgcccggcg agccacgcct ccagggtccc cacctcctcg cgcagcccgc gttcgatccc
      721 ctcgcggtcg accagcgcct tccgcgcctc ctcgagcttc ccgctcgccc ccgcggccgc
      781 ggccttcgcc tcctccaggg ccctttcggc cccgctcacc cgcacgtcca gctcgcgcgc
      841 cttctcgatc tcgggctttc gcgcctcgag gtccgccgtc gccagcgcga gcgtttcggt
      901 cgcgcccacg acttcgttct gcgtcgactc tcttctcttc cccgccgcct ccgcctcgcc
      961 ggcggccttc agcgaggtcg tcgcgcggct cctggcctcc tcctcgctct cctcggccgc
     1021 cttcgcctgc cgggccagct cctcgagcac cttctcccgc gccccgtgcg cctcgacctc
     1081 ggccgcggcc gcgacggcct ccgcttcgag gtgctcccgc tcctcggccg acaggaccgt
     1141 gacgctcgcg cgcagcgcct ccagctcacg gagccggtcc ttcgcttcct tcgccttccg
     1201 gtgggcggcg acggagatcc gctcgtagat ctcggcgccg gtgaggcgct ggaggaggag
     1261 cgaccgctcg tcggcgttcg ccttcaggaa gtcggcgaag tcgccctggg cgaggaggac
     1321 cgacttccgg aactggtcga aggtgaggcc gagcttgtcc tcgatcgcgg tcagcgtctc
     1381 ggtcttcgtg ccgccggccg tctggtcgac cgtgaggtcg acgagcgaga ggttctgctt
     1441 ctggaccttc ccgcccttct tcccgcgcgc ccggccgacc gtccacgtgg cccgccaccg
     1501 gtggtcgtcc cggccgacga agtcgacctg ggccatcgcg tccgtcgccc cgcggctcat
     1561 cagggaacgg acgtcgtagg ccgggagtcg ctcggcgtcg cccgcccgtc cgatctcggg
     1621 tccgccccgg gtgtcgagcc gcggggtcgc gtcgaagagg gcgaggcaga gcgcgtcgag
     1681 gagcgtgctc tttcccgcgc ccgtcggccc ggtgatggcg aagaggccgg agctcttcag
     1741 cggttccgtc tcgaagtcga gcgcgaactc gcccgagagg ctcgcgaggt tcttcccgcg
     1801 gatggcgagg atcttcacga cgcctcctcc tgcgaggctg cgtccacgag ctcgtggaag
     1861 gcggcctcca gctcggcggg caccgggtcg gcgttcccgc gctcccacag cttccggaag
     1921 accttctccg gggtgaggtc cgagagcgac ctctcggagc cgtcggccag ggtcacgccg
     1981 gagcccgccc tctgcggcgt gatcttcacg aggcgcgcgt ccttcccctc cagcgcctcg
     2041 gcgatctcgc cctggtaacc gaactccgac ccctcgagcc ggacccgcac ctccagccag
     2101 gggcgccggt cgggctctgc cgcctcgcgg ggcggagcgg gcgggagctt ctcgaggagc
     2161 gcgaggacct cggcgagcgg cgccggctcg ccgggaaggc gaaggatggg aacgctccgc
     2221 ggcacgcgaa cgctcttcac gtccttgcac cgcccgccct ccagctccac gaggcagacc
     2281 tggtgcgggt agacgtcctc gtcgagcgag agcggcaggg gcgagccgga atagcggacg
     2341 ttgccgcggc cgccgacttg ctgggggaag tggaggtggc cgagcgcgac gtagtcgacg
     2401 tcggccggaa agacgtcgac cggcagcggg tgctggttcc ctcccaggac cttccgctcg
     2461 ctctcgtccg agaggcgcat cccctcgacg tagcagtgcc ccgtcgcgag gagcgcctga
     2521 ccggccgtcc gcctcgagcg ggcggcctcc agcgctctcg cgtaaatcgc gcggacgccg
     2581 tcgacgatgc cttcgggcgt cgcctcccct ccgggcaggt cgcccagcct caggaacggg
     2641 acggccgcca cccacgccgc gacctggccg cccgggccgg aagagcgctc gcggaggggg
     2701 acgacgagcc gctcgacgtc gacttcagcc tcggcgcgcg gcaagaaccc gacgacgttc
     2761 accgagagag ccgcgaggac gggagcgggt gcctcgagcc gcgccggcga gtcgtggttg
     2821 cccgccacga cgacgacctg aagcccccgg caccggcggc tcgcgtcggc caggaagccg
     2881 taccagaggg ccagcgccga ggcgggcggg ttcgccccct cgaagacgtc gccgcagacg
     2941 aggagggcgt cgacgctctc cttctcgagg aggtcgagca gccagtcgag gaacgcccgg
     3001 tgctccgcct cgcggtcccg gtcgtagagg acgtgcccca ggtgccagtc ggccgtgtgg
     3061 acgatccgca tcactcctcc gtcggtcggg ccgggactcg gttggcgccg cccctgtact
     3121 cgtcgagaca tctcacgatt cgaggatcgc gcacaaggtc ggacacctgg cgtccctgcc
     3181 ccagatcgtg cccagcgaag ggcggctttc tccccgcacg ggccgtgagt ctctcgcgcc
     3241 ccggcccgcg cctcacctgt tcgcccctat cggcgccgcg gcacaccaca actggagagg
     3301 aatcgaaccg gcgagctcgg tgagcgctac gttagtcctt cggcggtcgc tccgaagagg
     3361 gccttcgcct cctcgtactc cctcgacagg tcgaagcccg tgtcggccga cgggtcgacc
     3421 agcttcatgc cgacgtccag gatgcagacg aggtggaggc cggagaactc ccccgggagc
     3481 gaccggagcc tgtactttcg cgcggggtcg ttcacgtcga agcccttcat gccgagcatg
     3541 gcgatctcga acgtgacctt ccgtaggccg ccgcggcgtc cgccgggata cggcgcaact
     3601 ccgcgatccg ctcgatcggg tctcgcggcg ccgtcacgcc tactccgccg cgtccggctg
     3661 gtcgaggagg aaccgccacg cggggacgac cgcgatcgtc ccggaggcgg tcacgatccg
     3721 ctcgctctcg ttcctcgtca cgatcgtccc ctccttcgtc cccagctcct ccatcgccgc
     3781 ctcgagcgcc gcgacctccc gctttcgcgt ctccgggtcg aagagcgtct cgcagacctg
     3841 gacgagcgcc tccgggcgac cgcgccccgg aacgacgaag tcgacctccc ggcccgacct
     3901 cgtcttgtag taggagacct ccggatgcgc ccggcgcagg gcgacgaaga cgaggttctc
     3961 gaggaggtgc ccggagttta cgaagaggcc ggaggagacc gacgtcacga gcgcgtggtc
     4021 cacgcagtag accttcttcg ggttcgtgtt ccggcgggcg agcgaggcgt caaagaggct
     4081 cacggtgtag aggaagtaag cgtcctcgaa ccactccagg tagctcgcga ccgccgcctt
     4141 cggcacgccg tgcccgaggg acttcaggta cccggtgagc cggttgaccg tgtagagcga
     4201 cgcggcgtgg tcgacgagcc agcgcgcgag gtcggagagc gccttcggat gggcgacgtc
     4261 gtgcctctcc acgaggtcgc ggtagaggat cgcgtggaag tactcctggt gcgtcttcac
     4321 gcgcagcgag cggtcgagcc ggatcacctc cgggaagccg cccgtctccc agtactctcc
     4381 gaacaccttc tcgacgagga gccgcttgcg cgtcgagagg gttccgctcg cctcgatccc
     4441 gcgcgcgtcg aggagctccc ggaacgagaa cgggaagagc tcccaggaga gcgcccggcc
     4501 tcgcatctgg gtcgccacct ccttcgagag catccgcgcc gaggagcccg tgagcgtcac
     4561 ccggcacttc tccgtccgga ggagccggtc gacgaacggc tcccagcccg gcacggcctg
     4621 gatctcgtcg aagaagcagt ggaccgtctc cttccccttc ttctccgggt agagcgcgta
     4681 gtacgcctcc gtgacgaggc ccgggccctc gcgggcgagc ccggcgaggc ggtcgtcgaa
     4741 gaagttcacg aggaggacgt cctcccgcgg gaccccctgc gcgaggaggc gctcgacctg
     4801 ctggagggcg agcgtcgact tcccgcagcg ccggacgccg atcaggatcg tcgccttccc
     4861 cgcgacgggc cgcaccgcga ggcggcgggg cacccccgtc tcgagagggg cctcctggag
     4921 gtcgaggatg agcgccttca gcgtctcgcg catgccacca cccggtaagg aacgttaccg
     4981 gatgtgatca taaccggtaa cgaacgtttc cggatgaggt ctcgtcccgc gaggcgccac
     5041 cttccggccc ctgccgtacc ccccgacctc cacgagctcc gccatcaccc gcacctcccc
     5101 ctcgtcttcc ggcgtcagga cgatcggctg gcggacggtg ttccggctcc gcgtccgaag
     5161 ctcgatccgc gtgtggagga agttcccgtc gtcgccccag accctctcgc ttcggtactc
     5221 cttcaccgtg aaggcgccgg ccgggtcggc ggagggcgac ccgtcgcgga cgaggacgat
     5281 ccggccgtgc ctcgagccgg cgcggcgcgc ggaagaggca ccaggcacgg tcggggatca
     5341 gcttgtccat cgagtcgcca gagacggcgg cgacgaacat cccgggctcg atccgccgcc
     5401 cctcgcccag ctcggcccac tcggagacga gggcgcccgc ggcctccgcg ccgaagagcg
     5461 cttcgctccc ggcgtgggcc gcggccttca ggtcggtgaa gagcgggacg cacgtccgga
     5521 agcggtccgc ctccggcggg tcctcgaccc tccggaaccg caggacggtc gtcttcgagg
     5581 ccgcctcggc ctccgccgcc gcctcgtcca ccccgccccg cgcgtgccgc tcgaggattg
     5641 ccggcagctg gaagaggtcg tggcagatgg cgaactcgtc cagggacgga tcgcgcttga
     5701 gcaccttcag cttctcggcc acgcgagcca tctccgccga gtccggggcg cagagctcgt
     5761 tcaggaagct gtcccggtac gaggtggtgt agcccatcag cgccccacgc ctcggcccgc
     5821 gccgccggaa atgcgttcgg cccgattctc gaagaccctc actcgctcct ccctcagggg
     5881 gccgaagccc ggagaccgtc gtggcgcgag aggcggcgtc cgtcgagggg ccgctcgact
     5941 cgagccgggc gaaggcacgg ggcgccggga cgaatccgcc gccagacgga ctctgcggtg
     6001 tggggtcgcg attgggaatg atgcggggag atcagcgggc ggcgattacg cgtggcgtcg
     6061 cgtctcaccc gttcggggga tgacagccgg cgccgatccg tgatcggagc ggcatcggac
     6121 ggcgtcatgc tccgcccccg ccttccgagc cgccctcctt cccttcctcc tcgcccccga
     6181 ccccgtagtc gaagccgtag acgtcggtgt accagacggg gccgcaggcc atgccgccgt
     6241 aggtgatctc gagggcgagg aggccgttgc cgtagcccgc gttcacgagg agggtgcgga
     6301 ggcgcgtgag atcgaggtcg tggatgcggc ggtgggcggt cctccgggtg aagtccggcg
     6361 agcggtactc gaagtcgccg acctcgccaa cgaagacgaa ggcgaggtgg cggcacggat
     6421 cggcgtgggt gaggccgtcg tcgtcgacgc gcaggaccgc gcggccgcag attgggcagt
     6481 ggggcctcgg cggcgtgccg ccgaacggct gctcgaccga ggcgagctgg ggcttcgacg
     6541 cggacttcat cggctcctcc tcctgcggcc cgcggccggg cgggcgcgtt cgaaggagag
     6601 ggtgcggcgc agggatggac agatggtgtc cgctggcggg cggagaggtg ggctacctct
     6661 tttctggcaa cctctccctc gcccgcggga cctcgatcag cgggacgcag ggtccggagg
     6721 ccttctttcc gcactcgctc gtgaggtaga gggtggcgtc gtccgagccg tagcagatcg
     6781 actcgccctg cttccgatga gggaggtcga ctcggcgcgg c
//
LOCUS       DAOTEZ010000221         6804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_7989_length_6804_cov_5.996296, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000221 DAOTEZ010000000
VERSION     DAOTEZ010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6804
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7989_length_6804_cov_5.996296"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="P5164_12205"
     CDS             <1..743
                     /locus_tag="P5164_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44695.1"
                     /translation="KKLNQWKSTRATAEVLYSLVHYLKAEGALGGRETMKVAVGGRET
                     SFTFEPDRYTGKKNQVVIPGREVDPKTTSTIAVEKGGKGFAFASAAWHFSTEKLPEAD
                     RGDFFNVSRKYFLRENTGKEWVLKPIADGAVLRPGDQVEVQISLRTKHEAEYVHLRDP
                     RGAGFEPETTVSRYKWDLGIAWYEEVRDSGTNFFFEQLPVGEYTFRYRLRANMAGTFR
                     VGPATVQSMYAPEFNAYSSGAVLSVKGE"
     gene            810..1067
                     /locus_tag="P5164_12210"
     CDS             810..1067
                     /locus_tag="P5164_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRY44696.1"
                     /translation="MKASIDKAGRIVIPASVRARAGLTPGTEVEILLDDVSVRLVRKV
                     RGPKLVRRGRRLVAQPTVRGRALPTVDLAALVEEERNRWPG"
     gene            1055..1534
                     /locus_tag="P5164_12215"
     CDS             1055..1534
                     /locus_tag="P5164_12215"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRY44697.1"
                     /translation="MARLTGVFLDTSVLLGGTINVGPTSRPAQAVMDAVAEGRLRRAR
                     TAWHCCLEYYAVATRLPEELRLAPADALRLLEEEILGRLEVVELPAAARLPFLRSAVA
                     ERVAGGRLYDAHIAEIARRSGSDTIVTDNVRHFRGRPESAVRVCSAAELTAELEAGS"
     gene            1646..2812
                     /locus_tag="P5164_12220"
     CDS             1646..2812
                     /locus_tag="P5164_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRY44698.1"
                     /translation="MKDRLSRRDLLALAATSGAVLALPRWARAAEAAPVKKKVLILGG
                     TRFLGVAIVEAALAKGWELTLFNRGKSNPGLFKGRPLEEIHGDRNVAEDVKKLAGRKW
                     DAVIDTSGYFPRQVRSAAEVLSGNVGQYVFISSISVYDAPMKAGMDETAAVARLAAGT
                     DVDAINEITGGNYGALKLLCEEAAEKAMPGKALNVRPGYIVGDRDGSDRFTYWPVRVR
                     KGGEVLVAGKPTDPLQFIDVRDLGDWIVRMVESGTNGVFNATGPKERLTMKAFLEACR
                     KETKSNATFTWAPEAKLKELGLTPEADFPIWVSTEGPEAGIGDVSIARALKAGLTFRP
                     LPETIASTLHWWDSLPEERRAKARAGLAAEKEAEVLAALKGKATGAAPAPEAKK"
     gene            2809..4287
                     /locus_tag="P5164_12225"
     CDS             2809..4287
                     /locus_tag="P5164_12225"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRY44699.1"
                     /translation="MTERRSATERRALLPFLVGASLLAAGVSQAAGPTEAEVASASAR
                     ITAASALATVEVLAAPGMAGRLSGTDGYRKAADHVVSELRKAGLRPPLDLPDHRQAFT
                     HGLAGIEAASLTLLPAEGEKDGTPYEAEVLKDWSPMVNGGSGDVTAEVVFVGYGFDAA
                     SEGRDDYAGVDVKRKVVLVLRGEPEGREWKDHRSTVARTKAAARKGAAGLLLIDSPVT
                     STSVGISRDLPEAMVSEAFADRLLAGKKLTVAELRKVLSKGGLASFETGSRLRFQVKG
                     RPWREVTTHNVVALLPGSDPSVKGEYVVVGAHLDHIGDWPRLNPGADDNASGSASLLE
                     VARAAAALKSRPRRTIAFVFFAAEELGLLGAEHFASHPPKGLEKCVAVLNMDMLGAGN
                     GLYVAGGENFPEIRAAMEGARDRLAPGFGLKSGRIRGEGRADHAPFFEKGIHAVSLFA
                     MGGDHHGYHSPDDTVYFVTPKTMESGARTVFGAAVALADAKP"
     gene            complement(4373..4702)
                     /locus_tag="P5164_12230"
     CDS             complement(4373..4702)
                     /locus_tag="P5164_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HRY44700.1"
                     /translation="MSTVHVTPVRTYLLVFFALMALTFLTVGVAHVNIAHHLPGQVTD
                     AINDAVAMAIAVTKATLVVLFFMHVWHSARLNKIVVWASLFFILILFAFSLADYFSRG
                     WLGVPGK"
     gene            complement(4719..5375)
                     /locus_tag="P5164_12235"
     CDS             complement(4719..5375)
                     /locus_tag="P5164_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HRY44701.1"
                     /translation="MASEARPAPLAHHFDDYDQQRESATLGMWLFLAQEVMFFGGLFL
                     AYTVYRWKYPAWFALGSNQLDVPLGLANTIVLIGSSLTMVLAVRAAQLGKARQIAGWI
                     GATMALGTGFLVVKGFEYHHKWVHHLIPGAGFHIEGTTDPAAQTFFVLYFCMTGLHAL
                     HMIVGLGLLAFLVPKALRGDFSAENHNWVEGVGLYWHFVDVIWIFLFPLLYLLGRHHA
                     "
     gene            complement(5365..>6804)
                     /locus_tag="P5164_12240"
     CDS             complement(5365..>6804)
                     /locus_tag="P5164_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRY44702.1"
                     /translation="FSSDVYNRLFTMHGILMIFFFLIPSIPAVLGNFLLPVMIGAKDL
                     AFPKLNLTSWYLYMIGGLMTFGSLLTGGIDTGWTFYTPMSTTFAQGQVLVTAVGIFVA
                     GFSSILTGLNFIVTIHRMRAPGMTWFRLPLFVWAHYATSIIQILGTPVVAIAILLVGA
                     ERLFHIGVFDPKYGGDPLLFQHLFWFYSHPAVYIMILPSMGVMSELVTAFSRKRVFGY
                     HFIAFSSLFIAVIGFLVWGHHMYVSGQSVVAGAIFSFLTMLVAIPSAVKVFNWTATLY
                     KGSISLQTPMLYALGFIGVFLIGGLTGVMLGTLGIDIHVHDTYFIVAHFHYVMVGSAI
                     MGYLGGLHFWWPKITGRMYDEFWSKVGALLIFVGFNTTFFPQFILGYLGMPRRYHMYP
                     AEFQTWNVLSSAGATILGVGYLIPVIYLLWSLKFGAKAPANPWGAKGLEWQTASPPPV
                     ENFATPPVVTEEAYAYETSAPAKEVTVGV"
BASE COUNT         1169 a         2223 c         2490 g          922 t
ORIGIN      
        1 acaagaagct gaaccagtgg aagtcgaccc gggcgacggc cgaggtcctc tactccctcg
       61 tccactacct gaaggcggaa ggggccctcg gcgggcggga gacgatgaag gtggccgtcg
      121 gcggacggga gacgtcgttc acgttcgagc cggaccgcta caccggcaag aagaaccagg
      181 tcgtgatccc ggggcgggag gtcgacccga agacgacctc gaccatcgcc gtcgagaagg
      241 ggggcaaggg cttcgcgttt gcctccgccg cctggcactt ctcgacggag aagctcccgg
      301 aggccgaccg gggagacttc ttcaacgtgt cgcggaagta cttcctccgc gagaacacgg
      361 ggaaggagtg ggtcctcaag ccgatcgccg acggcgcggt cctgagaccg ggggaccagg
      421 tcgaggtgca gatctcgctt cggacgaagc acgaagccga atacgtccac ctgcgcgacc
      481 cgcgcggagc cgggttcgag ccggagacga ccgtcagccg ctacaagtgg gacctcggga
      541 tcgcctggta cgaggaggtc cgcgacagcg gcacgaactt cttcttcgag cagcttcccg
      601 tcggcgagta cacgttcagg taccgcctcc gggcgaacat ggccgggacg ttccgggtcg
      661 gcccggcgac ggtccagtcg atgtatgccc cggagttcaa cgcctactcg agcggggcgg
      721 tcctttcggt gaagggagag tgagtccgag cgaaggcgcc gcgtggggca gggaaggcgg
      781 gcgtggcagc gcaatggcat actgatggca tgaaggcctc catcgacaag gcaggcagga
      841 tcgtcatccc cgcctccgtc cgcgcgcgcg ccggcctgac gcccggcacc gaggtggaga
      901 tcctcctcga cgacgtctcg gtccggctcg tgcggaaggt ccgcgggccg aagctcgtcc
      961 gacgcggccg gaggctcgtg gcgcagccga cggtccgggg gcgggcgctc cccacggtcg
     1021 acctggcggc gctggtcgag gaggagcgga accgttggcc cggctgacgg gcgtcttcct
     1081 cgacacgagc gtcctcctcg gcggaacgat caacgtcggg cccacgagcc gtccggcgca
     1141 ggccgtaatg gacgccgttg ccgagggccg gcttcgcagg gcgaggacgg cgtggcactg
     1201 ctgcctcgag tattacgccg tcgcgactcg ccttccggag gagctccgtc tggcgcccgc
     1261 cgacgcgctg cggctcctcg aggaggagat cctcgggcgg ctcgaagtcg tggaattgcc
     1321 ggcagccgcg cgcctcccgt tcctgaggtc ggccgtcgca gaacgggtcg ccggcgggcg
     1381 cctctacgac gcccacatcg ccgagatcgc ccgccgctcc ggctcggaca ccatcgtcac
     1441 cgacaacgtt cgtcacttcc ggggcaggcc ggagagcgcc gtgcgcgtct gctcggcggc
     1501 ggagctcacc gccgagctgg aggcgggctc ctgatccccg gaatcggaac tggtcggcag
     1561 agggtggtcc gagctcggag cgcccccggc tgaaacccgg gcgctcctct gccgtaccct
     1621 cggtaatgcc cgtggaggag caacgatgaa agaccgtctc agccgcagag atctcctcgc
     1681 cctggccgcg acgtccgggg ccgtcctggc ccttccgcgc tgggcccgcg ccgccgaggc
     1741 cgcgcccgtg aagaagaagg tcctcatcct cggagggacc cgcttcctcg gggtcgccat
     1801 cgtggaggcc gccctggcga aggggtggga gctgaccctc ttcaaccggg gcaaatcgaa
     1861 ccccggcctc ttcaaggggc gccccctcga ggagattcac ggcgaccgga acgtcgccga
     1921 ggacgtgaag aagctggccg gccggaagtg ggacgccgtc atcgacacgt ccggctactt
     1981 cccgcggcag gtccgctccg ccgcggaggt cctctcgggg aacgtcggcc agtacgtatt
     2041 catctcctcg atctcggtct acgacgcgcc gatgaaggcc ggcatggacg agaccgccgc
     2101 cgtcgcccgc ctcgcggcgg ggacggacgt cgacgcgatc aacgagatca ccggcgggaa
     2161 ctacggcgcc ctgaagctcc tctgcgagga ggccgcggag aaggcgatgc cggggaaggc
     2221 attgaacgtc cggcccggct acatcgtcgg ggaccgggac gggagcgacc gcttcaccta
     2281 ctggcccgtt cgcgttcgga aggggggcga ggtcctcgtc gccgggaagc ccaccgaccc
     2341 gctccagttc atcgacgtcc gcgacctggg cgattggatc gtccggatgg tcgagtccgg
     2401 gacgaacggc gtcttcaacg cgaccgggcc gaaggagcgg ctcacgatga aggcgttcct
     2461 cgaggcgtgc cggaaggaga cgaagtcgaa cgcgacgttc acctgggcgc cggaggcgaa
     2521 gctgaaggag ctcggcctga cgcccgaggc cgacttcccg atctgggtct ccaccgaggg
     2581 ccccgaggcc gggatcggag acgtctcgat cgcccgcgcg ctgaaggccg gactgacgtt
     2641 ccggccgctc ccggagacga tcgcctccac cctccactgg tgggactcgc tcccggagga
     2701 gcggcgcgcg aaggcgcggg ccggcctcgc cgcggagaag gaggcggagg tcctcgccgc
     2761 gctgaagggg aaggcgactg gcgccgcccc ggcccccgag gcgaagaagt gacggaacga
     2821 cggtccgcga cggaacggcg agcgctcctc ccgttcctcg tcggcgcgtc gctcctcgcc
     2881 gccggcgttt cgcaggccgc aggtccgacg gaggccgagg tcgcctcggc ctccgcgcgg
     2941 atcacggccg cgtcggcgct cgcgacggtc gaggtcctcg ccgcgcccgg gatggccgga
     3001 cggctcagcg ggacggacgg ctaccggaag gcggcggacc acgtcgtctc cgagctccgg
     3061 aaggcgggcc tccgcccgcc cctggacctc cccgaccacc gtcaggcctt cacgcacggc
     3121 ctggccggga tcgaggccgc gagcctgacg ctccttcccg ccgaggggga gaaggacggc
     3181 acgccgtacg aggcggaggt cctgaaggac tggtcgccga tggtgaacgg cggctccgga
     3241 gacgtcacgg ccgaggtcgt cttcgtcggc tacggcttcg acgccgcttc cgaagggcgc
     3301 gacgactacg ccggcgtcga cgtgaagagg aaggtcgtcc tggtcctccg gggcgagccc
     3361 gaagggcgcg agtggaagga ccaccgctcc acggtcgccc gaacgaaggc ggcggcgcgg
     3421 aagggagcgg cggggctcct cctgatcgac agccccgtca cctcgacgag cgtcggcatc
     3481 agccgcgacc tccccgaggc gatggtgagc gaggcgttcg ccgaccggct cctcgccggg
     3541 aagaagctga ccgtcgccga gctccgaaag gtcctctcga aagggggcct cgcctccttc
     3601 gagaccggga gccggctccg cttccaggtg aaggggcgcc cgtggcgcga ggtgacgacc
     3661 cacaacgtcg tcgccctcct cccgggctcc gatccgtctg tgaagggcga gtacgtcgtc
     3721 gtcggcgcgc acctcgacca catcggcgac tggccccgcc tcaacccggg agcggacgac
     3781 aacgcctccg gctccgcgag cctcctcgag gtcgcgcggg cggccgcggc gctgaagtcg
     3841 cgtccccggc ggacgatcgc gttcgtcttc ttcgcggccg aagagctcgg cctcctcggc
     3901 gcggagcact tcgcgagcca tcccccgaag gggctcgaga agtgcgtcgc ggtcctgaac
     3961 atggacatgc tgggcgcggg caacggcctc tacgtcgccg gcggggagaa ctttccggag
     4021 atcagggcgg cgatggaggg cgcccgcgac cgcctcgcgc ccggcttcgg tctgaagtcg
     4081 gggcgaatcc gcggcgaggg gcgcgcggac cacgcgccgt tcttcgagaa ggggatccac
     4141 gccgtctcgc tcttcgcgat ggggggcgac caccacggct accactcgcc ggacgacacg
     4201 gtctacttcg tcacgcccaa gacgatggaa tcgggggcgc gcaccgtctt cggggcggcg
     4261 gtcgccctgg ccgacgcgaa gccgtagccc cgcgcgatcg ctcatctccc gggacggcag
     4321 agggccccgg cgtcgccggg gccctctgct tctgcacgcc tcccctggcg gcctacttcc
     4381 ccggcactcc gagccagccg cggctgaagt agtcggcgag cgagaaggcg aagaggatca
     4441 ggatgaagaa gagcgacgcc cagacgacga tcttgttcag gcgcgccgag tgccagacgt
     4501 gcatgaagaa gaggacgacg agcgtcgcct tcgtcacggc gatcgccatc gcgaccgcgt
     4561 cgttgatcgc gtcggtgacc tggccgggga ggtgatgcgc gatgttcacg tgcgcgaccc
     4621 ccacggtcag gaaggtcagc gccatgagcg cgaagaagac gaggaggtag gtgcggacgg
     4681 gagtgacgtg gacggtcgac atggctactc cgtcggcctc aggcgtggtg ccgccccagg
     4741 aggtagagga gcgggaagag gaagatccag atcacgtcga cgaagtgcca gtagaggccg
     4801 accccctcga cccagttgtg gttctcggcc gagaagtccc cgcggagcgc cttcgggacg
     4861 aggaaggcga ggagcccgag gccgacgatc atgtggaggg cgtggagccc cgtcatgcag
     4921 aagtagagga cgaagaaggt ctgcgcggcg gggtcggtcg tcccctcgat gtggaagccc
     4981 gccccgggga tcaggtggtg gacccacttg tggtgatact cgaacccctt gacgacgagg
     5041 aagcccgtcc cgagcgccat cgtcgcgccg atccagccgg cgatctgccg cgccttcccg
     5101 agctgggcgg cgcggacggc gaggaccatc gtgagggacg agccgatcag gacgatggtg
     5161 ttcgcgaggc cgagcgggac gtcgagctgg ttcgagccga gggcgaacca ggcggggtac
     5221 ttccagcggt agacggtgta ggcgaggaag agcccgccga agaacatgac ctcctgcgcg
     5281 aggaagagcc acatcccgag cgtcgccgac tcgcgctgct ggtcgtagtc gtcgaagtgg
     5341 tgggcgaggg gggcggggcg ggcctcagac gccaacggtc acctccttcg ccggggcgga
     5401 ggtctcgtac gcgtacgcct cctccgtcac gaccggcggc gtcgcgaagt tctcgacggg
     5461 cggcggcgac gcggtctgcc actcgagccc cttcgcgccc caggggttcg cgggggcctt
     5521 cgccccgaac ttcagcgacc agaggaggta gatcaccggg atgaggtagc cgacgcccag
     5581 gatcgtcgcc ccggcggagg agaggacgtt ccacgtctgg aactccgcag ggtacatgtg
     5641 gtagcggcgc ggcatcccga ggtagccgag gatgaactgc gggaagaagg tcgtgttgaa
     5701 gccgacgaag atcaggagcg cgccgacctt cgaccagaac tcgtcgtaca tccggccggt
     5761 gatcttcggc caccagaagt ggaggccgcc gaggtacccc atgatcgcgc tgccgaccat
     5821 gacgtagtgg aagtgggcga cgatgaagta ggtgtcgtgg acgtggatgt cgatcccgag
     5881 cgtcccgagc atgacgcccg tcagcccgcc gatcaggaag acgccgatga agccgagggc
     5941 gtagagcatc ggcgtctgga gcgagatcga ccccttgtag agcgtggccg tccagttgaa
     6001 gaccttcacg gccgagggga tcgccacgag catcgtcagg aacgagaaga tcgccccggc
     6061 gacgacggac tgccccgaga cgtacatgtg gtggccccag acgaggaagc cgatgacggc
     6121 gatgaagagg ctcgagaagg cgatgaagtg gtacccgaag acccgcttgc gcgagaaggc
     6181 ggtgacgagc tcgctcatca cgcccatcga cggcaggatc atgatgtaga cggcggggtg
     6241 cgagtagaac cagaagaggt gctggaagag gagcgggtcg ccgccgtact tcgggtcgaa
     6301 gacgccgatg tggaagagcc gctcggcgcc gacgaggagg atcgcgatcg ccacgaccgg
     6361 cgtcccgagg atctggatga tgctcgtcgc gtagtgggcc cagacgaaga gcgggaggcg
     6421 gaaccaggtc attcccggag cccgcatccg gtggatcgtg acgatgaagt tgaggcccgt
     6481 gaggatcgac gagaagccgg cgacgaagat gccgaccgcc gtgacgagga cctgcccctg
     6541 cgcgaacgtc gtcgacatcg gcgtgtagaa cgtccagccg gtgtcgatgc cgcccgtcag
     6601 gagcgagccg aacgtcatca gcccgccgat catgtagagg taccagctcg tcaggttgag
     6661 cttcgggaag gcgaggtcct tcgctccgat catgaccggc aggaggaagt tcccgagcac
     6721 cgccgggatc gacgggatca ggaagaagaa gatcatcagg atgccgtgca tcgtgaagag
     6781 ccggttgtag acgtcggagg agaa
//
LOCUS       DAOTEZ010000222         6789 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_8011_length_6789_cov_8.429314, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000222 DAOTEZ010000000
VERSION     DAOTEZ010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8011_length_6789_cov_8.429314"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="P5164_12245"
     CDS             complement(<1..275)
                     /locus_tag="P5164_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44703.1"
                     /translation="MAAKSLLGWLRGARVREGDAGREADRLPPAEERRPGVTVHAASA
                     VAELGAGAARGAVSRLGPFAPPAAGDGRAWAAVVLSGPAAGDLFFAA"
     gene            430..1368
                     /locus_tag="P5164_12250"
     CDS             430..1368
                     /locus_tag="P5164_12250"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRY44704.1"
                     /translation="MNQKKTLSAALIAILIAVSGIALAGPQARIVGTVTTTKGEPIEG
                     ATVTITTPAFGKFKVVLTSDKDGKWGTILNDSTIAYDYLIEAKGYIPIKQNKKVGIGS
                     EGTLDVQLLTADQAVEKGLVKEVVDPYTLAFNGAVEKFQAGDLDGAIEGAKKATELGP
                     DKAGAWDMATKVAAARKDWDLVIQWGEKALSIEPDNGSLFGALMEAYKAKGDKAKAAE
                     YEKKFIAANPDKPEILYNQAVDLYNKGDFKGADPILRKVLEGAPEYANAHFLLGMSCV
                     NLNKIPDMKKHLEEYIRLDPKGKEVATAKEMLEAFK"
     gene            1394..2014
                     /gene="udk"
                     /locus_tag="P5164_12255"
     CDS             1394..2014
                     /gene="udk"
                     /locus_tag="P5164_12255"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HRY44705.1"
                     /translation="MTAAPLAIGVAGGTGSGKTTVANAIRNRVGEDRIAILSHDWYYR
                     DWVDLPKDVLDHKNFDHPDSLETDLLVRHLKALKDGATVEVPVYDFKTHRRAPETRRI
                     EPRKVVLVDGILIYAEPALRTLFDVRIFVDTDADVRLIRRIRRDIAERGRTLESVVSQ
                     YESTVRPMHLEFVEPSKRYADLIIPEGGENAVALEFLFARLEKILG"
     gene            2082..3182
                     /locus_tag="P5164_12260"
     CDS             2082..3182
                     /locus_tag="P5164_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44706.1"
                     /translation="MPIVVRFLSGSLTDRDFSFPGEVVRVGDAEGIDLRVDPAAPGDG
                     GARGRVIEISTEGAGFRIRSAGNRDLSAQGEFPLDHPVPDGGEVRFGAWGPVFTVRRV
                     SAELPFTAPPPLPAVEPARADDTASRRRAEDTGSRRRSVLTASGEKPVGPKTVMVMIQ
                     DALSKAREQGDSGLVEKGTVFVREVVADTITSATRSLKIGLLLTGAALVVLAFALGYN
                     VWRTRQTVAHVTRAADESVQGVRKELGTRVEEMRQERDALAAESAEVARKLGELEKTA
                     GASEQAVSDLRRRLGDADARRKDLEGRMQRALDALAADKEALQRQLEQMERERAADRM
                     RQQAEIEKLRQETAAPVPLPAEPAPTAPPAPR"
     gene            3241..4698
                     /locus_tag="P5164_12265"
     CDS             3241..4698
                     /locus_tag="P5164_12265"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRY44707.1"
                     /translation="MNVRPNLVGGYTILERLGRGGLSGVFRALRDADGLEVALKITSL
                     ADLDPEFRPQERFEREAELLGRLSHPALPKFHDWGVTLDGYGWLAMELVRGAPLSQFA
                     LRPAWELIPILIPIAESLAAVARGGIVHRDVAPDNVLVAMEEGRFAPKLIDFGVAKDL
                     FASAAAGGLTQHGAFLGKLSYAPPEQLLGLPKGQTLDFRADVYSFGLTVWELLAGRPA
                     VAATTLPEIVNAHLTRSWPRLDLPPARGGPAPKLLALVERMTARRREDRPSSWEEIVA
                     ALWQALDEVRPVHRELEQKQAELAPFDHVPPAEPRRNGAGADAPFPEAAAPPAGAPGG
                     DAPALAPAARLLTRELLLGRVVLIFGIAALAAALAFALHVVVSAPEPETEAGGLAPAT
                     DARGGRPASAAGTPAAAGRLLVALVPGGVLEEVVDEAGRRVAGPRPLPASLPLPPGAY
                     RARLAHPESGCALTVTFDVRPGGTTRVLESCLDGE"
     gene            complement(4705..5925)
                     /locus_tag="P5164_12270"
     CDS             complement(4705..5925)
                     /locus_tag="P5164_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HRY44708.1"
                     /translation="METEDLNRLIRSLNAAGEGPAGEEPLVEHVRELEAAEAVGPRTG
                     APIADGELSPRLAALLTETVRRGGTDLLLVPGAWPVLRLHGSLEPVSAAAGEPLGPSE
                     AFDLLSPSLSAERRRRYGAEGAVDLSVRLGALGRFRVNLHRTRRGTAAAIRVLPKRIP
                     PLAELGLPESLYDLTKAARGLLLVTGATGSGKTTTLAALVDRINRTQKRHVVTIEDPV
                     EYEHAHGASIVEQVEVGADAPTFAAALVAALRQDPDVILVGEMRDLETTRAALTAAET
                     GHLVLATLHTNDVPQTVYRITDIFPAEQQATVRQQLSLALSAVLCQQLVPRADGKGRV
                     VAAELMLATDGVRAHVRKGTFHQLHTELTLGRRFGMVTMEESLARLVRSGAITEAEAR
                     LRAAHPDDLAALLR"
     gene            6071..>6789
                     /gene="tyrS"
                     /locus_tag="P5164_12275"
     CDS             6071..>6789
                     /gene="tyrS"
                     /locus_tag="P5164_12275"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009369177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRY44709.1"
                     /translation="MPNFLPVPEQLELLLKGVETCVQKDELAAKLEASRKSGKPLRVK
                     VGFDPSAPDLHLGHTVVFRKMRHFQELGHEVVFLIGDFTGLIGDPTGKKATRPQLTKD
                     EVLANAETYKTQVFKILDPETTVVDFNSRWLGALGADGLVRLAGRYTVAQLLERDDFS
                     KRFKAGQPISVHELLYPLCQGYDSVFLEADVELGGTDQLFNLLVGRELMRSYGLAPQC
                     VMTTPLLVGLDGVEKMSKSLG"
BASE COUNT          980 a         2626 c         2344 g          839 t
ORIGIN      
        1 tccgccgcga agaagaggtc gcccgccgcc gggccggaga ggacgacggc ggcccacgcg
       61 cgcccgtccc ccgcggcggg aggggcgaac ggcccgaggc gcgagacggc gccccgcgcc
      121 gccccggcgc cgagctcggc gaccgccgac gcggcgtgca ccgtcacgcc cggacggcgc
      181 tcctctgcgg gcgggaggcg gtcggcctcc cgcccggcgt ccccttcgcg cacccgcgcg
      241 ccccgcagcc acccgagcag actcttcgcc gccacgcgcg gagtgtagag gcgagagccg
      301 gccctcgaaa ggaccgcgcc ggatgccgtc ccctgcggag tccggccgga gcggcccgca
      361 gccggccgag acggcacggc tttcgctaga atgacgcgtt ggtggcacgg tccacccccg
      421 gaggtagaga tgaaccagaa gaagacgctc tcggccgcac tgatcgcgat cctgatcgcc
      481 gtcagcggga tcgccctcgc gggcccccag gcccggatcg tggggacggt cacgaccacg
      541 aagggagagc cgatcgaagg ggcgaccgtg acgatcacga ccccggcgtt cggcaagttc
      601 aaggtcgtcc tgacgtccga caaggacggg aagtggggga cgatcctcaa cgactcgacg
      661 atcgcctacg actacctgat cgaggcgaag gggtacatcc ccatcaagca gaacaagaag
      721 gtggggatcg gctccgaggg gacgctcgac gtccagctcc tgaccgccga ccaggccgtc
      781 gagaaggggc tcgtcaagga ggtcgtcgac ccgtacaccc tcgccttcaa cggggcggtc
      841 gagaagttcc aggcgggcga cctggacggc gcgatcgagg gggccaagaa ggccaccgag
      901 ctcggacccg acaaggccgg cgcctgggac atggccacca aggtcgctgc cgcccggaag
      961 gactgggacc tcgtcatcca gtggggcgag aaggcgctct cgatcgagcc ggacaacggc
     1021 tcgctcttcg gcgcgctgat ggaggcctac aaggcgaagg gggacaaggc caaggcggcg
     1081 gagtacgaga agaagttcat cgccgccaac cccgacaagc ccgagatcct ctacaaccag
     1141 gccgtcgacc tctacaacaa gggcgacttc aagggcgccg accccatcct ccgcaaggtg
     1201 ctcgagggcg ccccggagta cgccaacgcc cacttcctcc tcggcatgtc gtgcgtgaac
     1261 ctcaacaaga tccccgacat gaagaagcac ctcgaggagt acatccgcct cgaccccaag
     1321 ggcaaggagg tcgcgacggc gaaggagatg ctcgaggcgt tcaagtgacg acccgcccgg
     1381 cggagggcgc cgcgtgaccg cggcgcccct cgccatcggc gtcgccggag ggaccggctc
     1441 cggcaagacg accgtcgcga acgccatccg caaccgcgtg ggagaggacc gcatcgcgat
     1501 cctctcccac gactggtact accgcgactg ggtcgacctg ccgaaagacg tcctggacca
     1561 caagaatttc gaccatcccg actctctcga gaccgacctc ctcgtgaggc acctgaaggc
     1621 gctgaaggac ggcgcgaccg tcgaggtccc cgtctacgac ttcaagacgc accggcgcgc
     1681 gccggagacg cgccggatcg agccccggaa ggtggtcctc gtcgacggga tcctcatcta
     1741 cgcggagccc gcgctccgga cgctcttcga cgtgaggatc ttcgtcgaca ccgacgccga
     1801 cgtgaggctc atccgccgca tccggcgtga catcgccgag cggggccgga cgctcgaaag
     1861 cgtcgtctcc cagtacgagt cgaccgtccg gccgatgcac ctcgagttcg tcgagccgtc
     1921 gaagcgctac gccgacctga tcattcccga ggggggcgag aacgccgtcg ccctcgagtt
     1981 cctcttcgcc cgcctcgaga agatcctggg ctgacccggg cgcgggcccc gggcggataa
     2041 tcccgatgca cattccgccg ccacgggcgg aggaggccgc catgccgatc gtcgtccggt
     2101 tcctctccgg gtccctcacc gaccgcgact tctcgttccc cggcgaggtc gttcgcgtcg
     2161 gcgacgccga gggcatcgac ctgagggtcg accccgccgc gcccggcgac ggcggcgccc
     2221 gcgggcgggt catcgagatc tcgacagagg gcgccggctt ccggatccgc tcggccggca
     2281 accgggacct ctccgcgcag ggcgagttcc cgctcgacca tccggtcccc gacggaggcg
     2341 aggtccgctt cggcgcctgg ggcccggtct tcaccgtgag gcgcgtctcc gccgagcttc
     2401 ccttcaccgc tccgccgccc ctccctgccg tcgagcccgc gcgcgccgac gacaccgcct
     2461 cccgccgacg ggccgaggac accggctcgc ggcgtcggag cgtcctgacc gcgtccgggg
     2521 agaagccggt cgggccgaag acggtcatgg tcatgatcca ggacgccctc tcgaaggccc
     2581 gcgagcaggg ggactccggg ctcgtcgaga aggggaccgt cttcgtccgc gaggtcgtcg
     2641 ccgacaccat cacgagcgcc acccgctcgc tgaagatcgg gctcctgctg acgggggcgg
     2701 cgctcgtcgt cctcgcgttc gcgctcgggt acaacgtctg gcgcacgcgc cagaccgtcg
     2761 cgcacgtcac gcgagccgcc gacgagagcg tccagggagt gcggaaggag ctcggcaccc
     2821 gcgtcgagga gatgcggcag gagcgcgacg cgctcgccgc ggagtcggcc gaggtcgccc
     2881 gcaagctcgg cgagctcgag aagaccgccg gcgcgagcga gcaggccgtc tccgacctcc
     2941 gccggcgcct cggcgacgcc gacgcccgcc ggaaggacct cgagggtcgg atgcagcgcg
     3001 cgctcgacgc gctcgcggcc gacaaggagg ccctccagcg gcagctcgag cagatggagc
     3061 gggagcgcgc cgccgaccgg atgcggcagc aggccgagat cgagaagctc cggcaggaga
     3121 cggccgcccc cgtcccgctc ccggccgagc cggcgccgac cgcccccccg gccccgcgat
     3181 gagggcgcgg ggcctcgccc cgcgccccgt ccccgggccg actcctgaga agatgctccc
     3241 gtgaacgtcc gcccgaacct cgtcggcggc tacaccatcc tcgagcgcct cggaagaggc
     3301 ggtctctccg gagtcttccg cgccctgcgc gacgccgacg gcctcgaggt cgcgctcaag
     3361 atcacgagcc tcgccgacct cgacccggaa ttccggccgc aggagaggtt cgagcgggag
     3421 gccgagctcc tcgggcgcct ctcccacccg gcgctgccga agttccacga ctggggcgtg
     3481 acgctcgacg gctacggctg gctcgcgatg gagctcgtcc gcggcgcccc gctttcccag
     3541 ttcgcgctcc ggccggcctg ggagctgatc ccgatcctga tcccgatcgc cgagtcgctc
     3601 gccgccgtcg cccgcggcgg catcgtccac cgcgacgtcg cccccgacaa cgtcctcgtc
     3661 gccatggagg agggccgctt cgccccgaag ctgatcgact tcggcgtcgc gaaggacctc
     3721 ttcgcctcgg cggcggccgg aggcctgacg cagcacggcg cctttctcgg gaagctctcc
     3781 tacgccccgc ccgagcagct cctcggcctc ccgaaagggc agacgctcga cttccgcgcc
     3841 gacgtctact ccttcggcct caccgtctgg gagctcctcg ccggccgtcc ggccgtcgcc
     3901 gcgacgacgc tgccggagat cgtgaacgcg cacctgaccc gctcctggcc gcgcctcgat
     3961 ctcccccccg cccgcggcgg ccccgcgccg aagctcctcg cgctcgtgga gaggatgacc
     4021 gcccgccgcc gcgaggaccg cccctcctcg tgggaggaga tcgtcgccgc gctgtggcag
     4081 gcgctggacg aggtgcgccc cgtccaccgc gagctcgagc agaagcaggc cgagctcgcc
     4141 ccgttcgacc acgtcccccc ggccgagcca cggcgtaacg gcgccggcgc cgacgcgccg
     4201 ttcccggagg cggcggcgcc cccggcgggc gcccccggcg gggacgctcc cgccctggcg
     4261 cccgccgcgc gcctcctgac gcgcgagctc ctcctcgggc gcgtcgtcct catcttcggc
     4321 atcgccgctc tcgccgccgc gctcgccttc gcgctccacg tcgtcgtctc cgcgccggag
     4381 ccggagacgg aggccggcgg cctcgcgccg gcgacggacg cgcgcggagg ccgccccgct
     4441 tcggccgccg gcacgcccgc cgccgccggc cggctcctcg tcgcgctcgt cccgggcggc
     4501 gtcctcgagg aggtcgtcga cgaggcgggc cgccgcgtcg ccggcccccg cccgctcccg
     4561 gcgagcctcc cgctccctcc cggcgcctac cgcgcccggc tcgcgcaccc cgagtcgggc
     4621 tgcgcgctga cggtcacgtt cgacgtccgc cccggaggga cgacccgcgt cctcgagagc
     4681 tgcctcgacg gggagtgacc cgccctaccg gaggagggcc gcgaggtcgt cggggtgcgc
     4741 cgcgcgcaga cgcgcctcgg cctccgtgat cgcgcccgag cggacgagcc gggcgagcga
     4801 ctcctccatc gtcaccatcc cgaagcggcg gccgagcgtc agctcggtgt gcagctggtg
     4861 gaacgtcccc ttccgcacgt gcgccctcac gccgtccgtc gcgagcatca gctccgccgc
     4921 cacgacgcgc cccttcccgt cggcccgcgg gacgagctgc tggcagagga ccgccgagag
     4981 ggcgagcgag agctgctggc gcaccgtcgc ctgctgctcg gccgggaaga tgtcggtgat
     5041 ccggtagacc gtctgcggga cgtcgttcgt gtggagcgtc gcgaggacga ggtgccccgt
     5101 ctcggcggcg gtgagcgccg cgcgcgtcgt ctcgaggtcg cgcatctcgc cgaccaggat
     5161 cacgtcgggg tcctgccgga gagccgccac gagcgccgcg gcgaacgtcg gcgcgtcggc
     5221 ccccacctcc acctgctcga cgatcgacgc gccgtgcgcg tgctcgtact cgaccgggtc
     5281 ctcgatcgtc acgacgtgcc gcttctgcgt ccggttgatc cggtcgacga gggcggcgag
     5341 cgtcgtcgtc ttccccgagc cggtcgcgcc cgtcaccagg aggagccccc gcgccgcctt
     5401 cgtcaggtcg tagagcgatt cggggaggcc cagctcggcg agcggcggga tccgcttcgg
     5461 caggacgcgg atcgccgcgg ccgtgccgcg ccgggtccgg tggaggttca cgcggaaccg
     5521 ccccagcgcg ccgagacgga cggagaggtc gacggccccc tcggcgccgt accgccggcg
     5581 gcgctcggcc gagagcgacg gggagaggag gtcgaacgcc tccgacgggc cgagcggctc
     5641 gccggccgcg gccgagaccg gctcgagcga gccgtggagg cggagcacgg gccacgcccc
     5701 ggggacgagg aggaggtcgg tcccgccgcg gcggaccgtc tcggtcagga gcgccgcaag
     5761 gcgcggggag agctccccgt ccgcaatcgg agcacccgtt cgcggcccga cggcctccgc
     5821 cgcctcgagc tcccgcacgt gctcgacgag cggctcctcc cccgccgggc cctccccggc
     5881 cgcgttcagg gagcggatga ggcggttgag gtcttccgtc tccacgccgg gagtgtacgg
     5941 atgacgcgcg cacaccgtcg cggcgctccc ggggggcgcg ctctccgggg gacgctgttc
     6001 gccgcccccc ggaccccccg agccggcagc aggacacgta cccgaccgtc cgggccacta
     6061 gaatccgcgc gtgccgaact tccttcccgt ccccgaacag ctcgagctcc tcctcaaggg
     6121 cgtcgagacc tgcgtccaga aggacgagct cgccgcgaag ctggaagcct cccgcaagag
     6181 cggcaagccg ctccgcgtca aggtcggctt cgacccctcc gcgcccgacc tccacctcgg
     6241 ccacacggtc gtcttccgga agatgcgcca cttccaggag ctggggcacg aggtcgtctt
     6301 cctcatcggc gacttcaccg gcctcatcgg cgacccgacc gggaagaagg cgacgcgccc
     6361 gcagctgacg aaggacgagg tcctcgcgaa cgccgagacc tacaagacgc aggtcttcaa
     6421 gatcctcgac ccggagacga ccgtcgtcga cttcaactcc cgctggctcg gcgcgctcgg
     6481 ggccgacggc ctcgtccgcc tcgcggggcg ctacaccgtc gcccagctcc tcgagcggga
     6541 cgacttctcc aagcgcttca aggcgggcca gccgatctcc gtccacgagc tcctctaccc
     6601 gctctgccag gggtacgact cggtcttcct cgaggccgac gtcgagctcg gcggcaccga
     6661 ccagctcttc aacctcctcg tcggccgcga gctgatgagg agctacggcc tcgcccccca
     6721 gtgcgtgatg acgacgccgc tcctcgtcgg cctcgacggc gtcgagaaga tgtcgaagtc
     6781 gctcggcaa
//
LOCUS       DAOTEZ010000223         6761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_8050_length_6761_cov_5.874739, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000223 DAOTEZ010000000
VERSION     DAOTEZ010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6761
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8050_length_6761_cov_5.874739"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="P5164_12280"
     CDS             complement(<1..1151)
                     /locus_tag="P5164_12280"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HRY44710.1"
                     /translation="MIRSARRPLSALVLSLAQLLVPGRLSGQTAAPAAPAVPTDAAAL
                     VEKHVAWLGGREALDGLRDLSLDGTIRVAGLSGSLSVRARRDDRQRTEVDLKVLRSVE
                     VLSGEDGWERNASGQIEPMGREKVTRLRRTLGRTFGRHLRGDGVVTSVAGTETRGGRT
                     WSVLRFTYPDGDVCDVLVDPATGESRYERSIVDGRTTWTTSSDVRVVNGLRIAARQEI
                     EGETARQAQSLTWETIAGNRGLDDTLFSPPAPAGSLVRLPTGRTTTDWVPVDLYRKRY
                     VYLAGELNGVATDVVLDSGAGMTVVDRALAEKLKLRPEGELEARGTGGNVGAGLVAGL
                     TLKVAGVEIAPISAAVIDLSDVGRRLGRPLPVILGKELFHALVVDLDYPG"
     gene            complement(1148..2020)
                     /locus_tag="P5164_12285"
     CDS             complement(1148..2020)
                     /locus_tag="P5164_12285"
                     /inference="COORDINATES: protein motif:HMM:NF014080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY44711.1"
                     /translation="MTTNIDESVTNALQALGFSEIEALVYAFLVPGEAATGYRISHAI
                     GKPTANTYKAIASLVQQGAVQVDEGGNRLIRAVPPEELLAGLDRRAREKREAARAALA
                     RRSSSTADERVYTLRARDQVLERARAMLGRACEIVLADLFPGPLALLAPELEAAASRG
                     VRVVVKTYGRRDLPGVAEVVEPDFRRALTEWPGQQLSLVVDAEEHLLALFDPALEAVH
                     QAVWSRSTFLSCLHHNHVAMELLHTAWKARDSLTDSDALVGISLLSSRPPGLRQLKER
                     TGRGDGVTEPGEPE"
     gene            complement(2124..3632)
                     /locus_tag="P5164_12290"
     CDS             complement(2124..3632)
                     /locus_tag="P5164_12290"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRY44712.1"
                     /translation="MPLRVSDPDSTPAEGRLSEAEERFERRRRGVGLVLGPLAFFVLL
                     AFPPAALPVAAAKLLAVVAWVLVWWITEAAPLPATAILGPALTVVLGVASAKEAFAAF
                     GDPIIFLFLGSFLLAGAMSAHGLDRRIARAILAVPAASASPRRVVVTFLALSAALSMW
                     LSNSATTAMLYPIALGTLGSFGEGAGRSPLARALLLACAYGASVGGIGTPVGSPPNLV
                     AIGQLSTLAGIRVGFVPWLLVALPILLAMVAGTALVLRTREGAPGPPLPVAAEPEAKR
                     PWAAGERSVAVAVLATVACWIGPGLLALVLGAEHPVALSLGRALPEGAVAVLGASLLF
                     VLPGGAGRGRAMGWNEAAKIDWGTLLLFGGGLSLGAAMFRTGLSEALGNGLVGATGVS
                     TLTGLTIVFSVFSIFFTEVTSNTAAATMLVPLAIASAQAAGVSPLEPSLGCALGCSMA
                     FMLPVATPPNAIVYGSGLVPVTQMVRAGFRLNLVACVVIPAGVLLLVPLVLR"
     gene            3721..4443
                     /locus_tag="P5164_12295"
     CDS             3721..4443
                     /locus_tag="P5164_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HRY44713.1"
                     /translation="MHASASVPARFSRHRELHRTDRVGWLRASVLGANDGLISTASLV
                     VGVATAGSPSGTVAVSAVAGLLAGAMSMAAGEFVSVSSQADTERADLARERHELETDE
                     AGEIAELAGIYVTRGLDHELAERVAVQLMEHDALGAHARDELGMHEATAARPLQAALS
                     SAAAFAAGAVVPALAAAFVPARLLVPVVVAATLLLLVGLGGLAAKVGGASVTTGAVRV
                     TFWGALAMAVTAAAGALFGAVG"
     gene            4502..5041
                     /locus_tag="P5164_12300"
     CDS             4502..5041
                     /locus_tag="P5164_12300"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HRY44714.1"
                     /translation="MPRYVGFLRAVNVGGRVVKMERLRQLFEETGFTGVSTHLASGNV
                     LFTAGRGRSATHEAKLERALGAGLGWEVATFLRTPAEMAAVVERLPFPAEDVATAHAV
                     YVGFLKAPPAPEAHALLHGLRTPTDALEVHGREVYWLCRVRTAASELSGASLEKALKL
                     AATFRNMTTVRAVAAKLAL"
     gene            5187..6065
                     /locus_tag="P5164_12305"
     CDS             5187..6065
                     /locus_tag="P5164_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44715.1"
                     /translation="MRIAKGLGALAIFLSAAVSPAHALAQSREETQWIVTTARGTGAR
                     GQTFYSTVHLFNPHTVDVSATWTFLPQSVLDPYGRALGENVNAPSATVVVPAGKAIDL
                     TSLWPASGAGAAGAVRIASIQPEGQPEPLPVTAHSVTSVYAPGTVRVVAGPFIAGQGE
                     ASLLRAGEIGRIRSLLTGYSETASYRSNVVLLSTDPVEETELTLTLFDHSGESRGSRS
                     ITLGRLAQTQVNDVADVFDYVLCLHGCPSASPFPEAFEMEIRVLSGGPVAAAGIVLEN
                     ETGSTTFVPALRTAAP"
     gene            6187..6690
                     /gene="tpx"
                     /locus_tag="P5164_12310"
     CDS             6187..6690
                     /gene="tpx"
                     /locus_tag="P5164_12310"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HRY44716.1"
                     /translation="MERTGVVTFLGNPLTLVGPEIRAGEKAPGFTVLDNGLAPKTLAD
                     FAGKTVVLSVTPSLDTPVCDAQLRRFNEAATKLGDDVVVLNVSMDLPFANKRFCSVAG
                     IEKALTLSDHRDASFGNAYGVLVKELRLLARAIFVVGKDGLVKYAEIVPEMTHHPDYD
                     KALAAAK"
BASE COUNT          974 a         2530 c         2383 g          874 t
ORIGIN      
        1 ccggggtagt ccaggtcgac gacgagggcg tggaagagct ccttgccgag gatgacggga
       61 agcggccgcc cgagccttcg cccgacgtcg gagaggtcga tgacggcggc cgagatcggg
      121 gcgatctcga ctccggcgac cttcagcgtc agcccggcga cgagccccgc gcccacgttc
      181 ccgcccgtgc cgcgcgcctc gagctccccc tcggggcgca gcttcagctt ctccgcgagc
      241 gcccggtcga cgacggtcat cccggccccg gaatcgagga cgacgtcggt ggctacgccg
      301 ttcagctctc ctgccaggta gacgtatcgc ttccggtaga ggtccaccgg gacccagtcc
      361 gtcgtcgtcc ggcccgtcgg gaggcggacg agcgacccgg ccggggccgg cggggagaag
      421 agcgtgtcgt cgagcccgcg gttcccggcg atggtctccc acgtcaggct ctgcgcctgg
      481 cgggcggtct ccccctcgat ctcctgccgg gccgcgatgc gaagcccgtt cacgacccgg
      541 acgtccgagg aggtcgtcca ggtcgtccgg ccgtccacga tgctcctctc gtacctcgac
      601 tcccccgtcg ccgggtcgac gaggacgtcg cagacgtcgc cgtcggggta ggtgaagcga
      661 aggaccgacc acgtgcgtcc accgcgcgtc tcggtcccgg ccacgctcgt gacgacgccg
      721 tccccgcgca ggtgacgtcc gaaggtccgg ccgagcgttc gccgcagccg tgtcactttt
      781 tcgcgaccca tcggctcgat ctgcccgctc gcgtttcgct cccacccgtc ctctcccgag
      841 aggacctcga cgcttcggag gaccttcaag tcgacctccg tccgttggcg atcgtctcgc
      901 cgcgccctga ccgagaggct gccggagagg ccggcgacac gaatcgtccc gtcgagggag
      961 aggtcgcgga gaccgtccag ggcctcccgc ccgccgagcc acgcgacgtg cttctcgacg
     1021 agggccgcgg cgtcggtcgg aacggccggt gccgcgggcg ccgccgtttg accggagagg
     1081 cgccccggga cgagcagctg cgcgagggag aggacgaggg ccgagagggg tcgacgtgcg
     1141 ctgcggatca ttcgggttct ccgggctccg tcaccccgtc gccccgtccg gtccgctcct
     1201 tcagctgccg cagcccgggc ggccggctcg agaggaggga gatcccgacg agggcgtcgg
     1261 agtcggtgag cgagtcgcgg gccttccacg cggtgtggag gagctccatg gcgacatggt
     1321 tgtggtggag gcaggacagg aacgtgctcc ggctccagac cgcctggtgg acggcctcga
     1381 gcgcggggtc gaagagggcg agcaggtgct cctcggcgtc gacgacgagg gagagctgct
     1441 ggccgggcca ctcggtcaaa gctcttcgga agtcgggctc gacgacctcc gcgacgcccg
     1501 gcaggtccct gcggccgtag gtcttcacga cgacccggac accccgggag gcggccgcct
     1561 cgagctcggg cgcgagaagg gcgagcggcc ccggaaagag gtccgccagg acgatctcgc
     1621 aggccctccc gagcatcgcg cgggcacgct cgaggacctg gtcgcgagcg cggagggtgt
     1681 agaccctctc gtcggccgtc gacgaggatc gccgggcgag cgcggcccgg gccgcctcgc
     1741 gcttctcccg cgcccgccga tcgagacccg ccagcagctc ctcgggcggc accgccctta
     1801 tcaaccggtt ccccccctcg tcgacctgga ccgccccctg ctgcacgagg gaggcgatcg
     1861 ccttgtaggt gttcgccgtc ggcttcccga tcgcatggct gatccggtag ccggttgccg
     1921 cctcccctgg gacgaggaac gcgtagacga gcgcctcgat ctcggaaaac ccgagtgcct
     1981 ggagtgcatt cgtgacagac tcgtcaatat tagtagtcat tacaactact aaatggtggg
     2041 gcgatcgcgg cggcttgtca aggggcgacc ggccggcgaa gcgctgtcgc gacccgaatt
     2101 ccgtcggtcg gacgatccgt cgttcagcgc aggacgaggg gcacgaggag aaggacgccg
     2161 gccgggatga cgacgcaggc gacgaggttc agccgaaagc cggcccggac catctgcgtg
     2221 accgggacca gccccgagcc gtagacgatc gcgttcggcg gcgtggcgac cggcagcatg
     2281 aacgccatgc tgcagccgag cgcgcagccg agcgacggct ccaggggcga gacgccggcc
     2341 gcctgagcgc tcgcgatcgc gaggggaacc agcatcgtgg cggcggcggt gttcgacgtc
     2401 acctcggtga agaagatcga gaagacgctg aagacgatcg tcaggcccgt cagggtcgag
     2461 acgcccgtcg ctccgacgag gccattcccg agcgcctcgg agagtcccgt ccggaacatc
     2521 gcggcgccga gggagagccc tccgccgaac aagaggagcg tcccccagtc gatcttcgcc
     2581 gcctcgttcc agcccatcgc ccggccgcgg cccgcgccgc ccggcaggac gaagaggagg
     2641 ctcgcgccga ggacggccac ggctccctcg ggcagcgcac gaccgaggga gagagcgacg
     2701 gggtgctccg cgccgaggac gagcgcgagg agcccgggtc cgatccagca ggcaacggtc
     2761 gcgagaacgg ccacggcgac gctccgctcc cccgcggccc agggccgctt cgcttcgggc
     2821 tccgccgcca cgggaagcgg cggtccgggc gctccctctc tcgtcctcag gacgagcgcc
     2881 gtacccgcga ccatcgcgag gaggatcggc agcgcgacga ggagccaggg gacgaagccg
     2941 acgcggatcc cggcgagcgt cgagagctgc ccgatcgcga cgaggttcgg tggcgacccg
     3001 acgggcgtcc cgattccccc gacggaggcg ccgtaggcgc aggcgaggag gagggcccgg
     3061 gcgagcggcg agcgacccgc cccctctccg aaagacccga gcgtgccgag ggcgatcggg
     3121 tagagcatcg ccgtcgtcgc cgagttcgaa agccacatcg agagcgcggc ggagagggcg
     3181 aggaacgtga cgaccacgcg gcgcggggac gcgctcgccg ccgggaccgc caggatcgcg
     3241 cgcgcgatcc ggcggtccag cccgtgggcg ctcatggcgc ccgcgaggag gaacgacccg
     3301 aggaagagga agatgatcgg gtctccgaac gccgcgaacg cctccttcgc cgacgccacc
     3361 ccgaggacga cggtcagcgc cgggcccaga atggcggtcg cgggaagagg cgccgcctcg
     3421 gtgatccacc agacgaggac ccaggcgacg acggcgagga gcttcgccgc cgcgaccggc
     3481 agcgcggccg gagggaacgc gaggaggacg aagaaggcga gcgggccgag gacgaggccg
     3541 acgccccgcc tcctcctctc gaaacgctcc tcggcttcgg agaggcgacc ctcggcgggg
     3601 gtcgagtcgg gatcggagac gcggagcggc aacgtcgcgc ccgagtctat gcccgcgccg
     3661 ccgcgccgcc gcggcggacg gctccgcgcg aggggagggc cactccgcta tcgttcggcc
     3721 atgcacgcct cggcctccgt ccccgcccgc ttctctcgcc atcgcgagct ccacaggacc
     3781 gaccgcgtcg ggtggctccg ggcctcggtc ctcggcgcca acgacgggct catctccacg
     3841 gccagcctcg tggtcggagt cgccacggcc ggctccccgt ccggcaccgt cgccgtctcc
     3901 gccgtcgcgg ggctgctcgc gggggcgatg tcgatggccg ccggcgagtt cgtctccgtc
     3961 agctcgcagg ccgacacgga gagggccgac ctggcgcggg agcggcacga gctcgagacg
     4021 gacgaggcgg gcgagatcgc cgagctcgcc gggatctacg tcacccgcgg cctcgaccac
     4081 gagctcgccg agcgggtcgc cgtccagctc atggagcacg acgccctcgg cgcccacgcc
     4141 cgcgacgagc tcgggatgca cgaggcgacc gccgcccggc cgctccaggc cgccctctcc
     4201 tcggccgccg ccttcgcggc gggggccgtc gtccccgcgc tcgccgcggc cttcgtcccc
     4261 gcgcggctcc tcgtcccggt cgtcgtcgcg gcgacgctcc tcctcctcgt cggcctcggc
     4321 ggcctcgccg cgaaggtcgg cggcgcctcg gtgacgaccg gggcggtccg cgtcacgttc
     4381 tggggagccc tcgccatggc cgtcacggcg gccgccggcg cgctcttcgg cgccgtcggc
     4441 tgatcggccg gccgcgaggg gccgcccggg agaatcgaga cctgacccct ataatcgacc
     4501 catgccccgc tacgtcggat tcctccgggc cgtgaacgtc ggcgggcggg tcgtgaagat
     4561 ggagcgcctc cggcagctct tcgaggaaac cggcttcacg ggggtctcca cccacctggc
     4621 gagcgggaat gtcctcttca ccgcgggccg cggccggagc gcgacgcacg aggccaagct
     4681 cgagagggcg ctcggggcgg ggctggggtg ggaggtcgcc acgttcctcc ggaccccggc
     4741 cgagatggcc gccgtcgtgg agaggctgcc gttccctgcc gaggacgtcg cgacggcgca
     4801 cgcggtctac gtcggattcc tcaaggcccc tcccgctccc gaggcgcacg ccctcctcca
     4861 cggcctccgg acccccacgg acgccctcga ggtccacggc cgggaggtct actggctctg
     4921 ccgcgtccgg acggccgcct cggagctctc gggggccagc ctcgagaagg ccctgaagct
     4981 cgccgccacg ttccggaaca tgaccaccgt gcgcgccgtc gcggcgaagc tcgccctctg
     5041 aggccctccc ccgggcggtc gtcggcccgg cgatcggagc ggcgggctcc cgctcgaccc
     5101 tgccgcagct cccgtgacgc cgatcacacc cggccggcga aaggtcccgc cccgccgcga
     5161 cactttccga gccagaggtg aatcacatga gaatcgcgaa gggcctcggc gctcttgcca
     5221 tctttctttc cgccgccgtc tccccggcgc acgcgctcgc acagtcgcgc gaggagaccc
     5281 aatggatcgt gacgacggct cggggcacgg gtgcgcgcgg ccagacgttc tactcgaccg
     5341 tccacctgtt caatccgcac acggtcgacg tctcggcgac ctggacgttc ctcccgcaga
     5401 gcgtgctcga cccgtacggc cgggccctcg gcgaaaacgt gaacgccccc tccgcgaccg
     5461 tcgtcgtccc cgccggcaag gcgatcgacc tcacctccct ctggcccgcc tccggcgccg
     5521 gcgccgcagg agccgtccgg atcgcatcga tccagcccga ggggcagccg gagcctctcc
     5581 ccgtcacggc gcactcggtg acgagcgtct acgcgcccgg cacggtccgc gtcgtcgccg
     5641 gtccgttcat cgccggccag ggagaggcgt cgctcctccg ggccggggag atcggccgca
     5701 tccgctccct tctgaccggg tactccgaga ccgcctcgta ccggagcaac gtcgtcctcc
     5761 tctcgacgga ccccgtcgaa gagacggagc tgacgctcac ccttttcgac cactccgggg
     5821 agtcgcgcgg aagccggtcg atcacgctcg gccggctcgc gcagacccag gtcaacgacg
     5881 tcgccgacgt cttcgactac gtcctctgcc tgcacggctg cccctcggcg agcccgttcc
     5941 ccgaggcctt cgagatggag atccgcgtcc tcagcggcgg cccggtcgcc gcagcgggca
     6001 tcgtcctcga gaacgagacg ggctcgacga cgttcgtgcc ggcgctccgg accgcggcac
     6061 cgtaggcgtt cggacggcgg acggggcgcc ggccgtcgcg tccgacggct cggcgccccc
     6121 gctccccggc cccggaggag cgatactccg gtcgagctcc aacccggacg aaagggagag
     6181 acctgcatgg aaagaacagg agtcgtcacg ttcctcggca accccctcac cctcgtcggc
     6241 cccgagatcc gggccggcga gaaggccccc ggcttcaccg tcctcgacaa cggcctcgcc
     6301 ccgaagacgc tcgccgactt cgccgggaag acggtcgtcc tcagcgtcac gccgtccctc
     6361 gacacccccg tctgcgacgc gcagctccgc aggttcaacg aggcggcgac gaagctcggc
     6421 gacgatgtcg tcgtcctgaa cgtctcgatg gacctcccgt tcgcgaacaa gcgcttctgc
     6481 tcggtcgccg gcatcgagaa ggcgctcacg ctgagcgacc accgcgacgc ttcgttcggg
     6541 aacgcgtacg gcgtcctcgt caaggagctc cgcctcctcg cccgcgcaat cttcgtcgtc
     6601 ggcaaggacg gcctcgtcaa gtacgccgag atcgtccccg agatgaccca ccaccccgac
     6661 tacgacaagg cgctcgccgc cgcgaagtag cgttctcttc gcggagacag ccgcgccggg
     6721 ccgccgcgac gcgagggcgc ggcggcccgc tttcgtctcc g
//
LOCUS       DAOTEZ010000224         6677 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_8194_length_6677_cov_5.709000, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000224 DAOTEZ010000000
VERSION     DAOTEZ010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6677
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8194_length_6677_cov_5.709000"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="P5164_12315"
     CDS             complement(<1..189)
                     /locus_tag="P5164_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44717.1"
                     /translation="MDLPVGGALPEGALEDGRQLGPEEPGGTQDPGVRREIVYYGTGL
                     LSEDVKLSRNPPETTGNRR"
     gene            425..1690
                     /locus_tag="P5164_12320"
     CDS             425..1690
                     /locus_tag="P5164_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRY44718.1"
                     /translation="MTAAPSPGEGMLAPLRDLLPELEAAYKDVHAHPELSMQEHRTSQ
                     LAADRLRAAGFEVVAGVGGTGVVGLLRNGSGPTVMLRADMDALPVREATGLPYASRET
                     AVDREGNSVPVMHACGHDMHVAWLLGAAALFARSRDAWRGTLLAVFQPAEETGEGARA
                     MIDDGLFGRFPKPDAILAQHVMVGPAGHLSSRPGVVTSAGDSLQVRMFGRGAHGSMPQ
                     ASVDPVVLAAATVLRLQTIVSREIAATDAAVVTVGSLQAGTKENVIPDEAVLKLNVRT
                     FDESVRARVLASIARIVDAEAAASGAPRKPEITVLDSYSLVRNDPEATRRVADALRQH
                     FPADRVQEAQPTTASEDFGCFGSEWHCPSVFWFVGGTDPDIYAREKADGRVGEIPTNH
                     NPRFAPVIHPTLEAGVEAMVVAALAWLAA"
     gene            1687..2373
                     /locus_tag="P5164_12325"
     CDS             1687..2373
                     /locus_tag="P5164_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HRY44719.1"
                     /translation="MTGVEPALASTLLAAFDLAGTLVFALSGATKAVQHRLDFFGVMV
                     LCFAAGNSGGIARDVMIGAVPPAALSHWGYIAVSLFAGGVTFFWYPLIDRLRSPVLLF
                     DAAGLALFVVSGTDKALAYHAGPVAAVLFGTLTGIGGGMVRDVLVGETPAVLKTDVYA
                     LAALVGAGALVAGRTLHLPAPVASILGATLCFGIRLGAMRCGWRLPSARPDGPAATGH
                     TRARREEHDR"
     gene            2354..5152
                     /locus_tag="P5164_12330"
     CDS             2354..5152
                     /locus_tag="P5164_12330"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRY44720.1"
                     /translation="MKSTIGETPRPAGPWQRSARELLEELGCANGAGLSAAEAAARLG
                     RFGTNELTERAGRSRLEILREQLLNVMTVVLVVAAALSALLGDWVEAVVILAIVVLNA
                     ALGYSQEYRAERSMAALKRMSVPTVRARRDGRVQEVSSRELVPGDVVLLETGNVVPAD
                     GRVLSSVNLRAQEAALTGESEAVEKDAEVVLEPERPLAERRNMVFAGTVVTWGHGEAV
                     VTATGMATELGKIASLIQSVVEEKTPLQQRLDRMGKGLAIGAGVLVAVVFLLGIRSAG
                     TREEVFEVLLTAVSLAVAAIPEAMTAVVTIALSLGAQRMLRRRALIRKLPAVETLGSV
                     GVICSDKTGTLTQNRMTVTALDVADHRIDLVQREGTEHFSLRRVGAGEAEPELAPTLD
                     LLLVGGALCNDAALVKEEGADGAFHAVGDPTEGSLVLAAAEYGARKDELDRALPRVAE
                     LQFDSERKRMTTVHRLPAGDAELPPALRPLWARRVLPEETPRFLAVTKGAIDGLLGKT
                     RSVWSGESAVPLDDEARRRVMAAHDDLAAKGMRVLGLAVRPLGGPPSAAETASLEEEL
                     LLVGLFGMLDPARPEVADAVSRCRAAGIRPIMITGDHPLTARHIARQVGITEGDEFRT
                     GADIDRLSDAELARAVGEVPVFARVSPEHKLRLVASLQAQGHVVAMTGDGVNDAPALR
                     KADIGVAMGITGTDVSRDAAEMVLLDDNFATIVAAVEEGRKIYDNIRKFIKYLLSCNA
                     SEIAVMLLAPLFGMPLPLLPLQILWMNLVTDGLPALALGVEPAEADIMKRPPASSEES
                     IFGRGLVPFVLVMGVVMSLISLGIGLWAYRSGDPAWQTLLFTTLIFSQVILALEVRSE
                     KASLLTLGLLTNPLMVAAFLSTVGLQVAVVYAPFLQRVFSTAPLGARDLGIAFGSGLA
                     VLLVVEAWKAVRRRRGGP"
     gene            5187..>6677
                     /locus_tag="P5164_12335"
     CDS             5187..>6677
                     /locus_tag="P5164_12335"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRY44721.1"
                     /translation="MTHPSRPPNRLASEPSPYLRQHAHNPVDWYPWGEEAFEKARRED
                     RPLFLSIGYSACHWCHVMEHESFEDEGVAALLNERFVSVKVDREERPDVDDVYMSAVQ
                     TMVGRGGWPLSAFLFPDGRPFFGGTYFPREDRRGQAGFRTILEKLSEAWRERREELSK
                     GADEIVAEVERAGRITDRAGREPLTARTGSVLTETLKRTFDARHGGFGGAPKFPPHLA
                     LEWLLLKGVEGDSIARSMALKTLDEMALGGIHDHLAGGFHRYSTDERWLLPHFEKMLT
                     DNAQLLALYARAFVLTGRDLYWRTARGIGDWLLSEMRGEEGGFFAATDADSEGEEGRY
                     FVFTEEEVRSVAGDDAAFFASAYRVRPEGNFHDESTGRATGRNVLHLAGEPSSEEERR
                     LAPIRARLLERRRLRVPPGTDDKRIAGWNALAVSGLAVASSILGEPRYLDAARASAEF
                     LLTRMRDASGRLFRTWKEGEARIPAFLEDEAFLALALLDLAEAEPEP"
BASE COUNT          872 a         2421 c         2442 g          942 t
ORIGIN      
        1 acgacgattg cctgttgtct cgggcggatt ccgggaaagc ttcacatcct ctgagaggag
       61 gcccgttccg taatacacga tttcacgcct gacccctggg tcctgggtcc cgcccggctc
      121 ctccggcccg agctgacggc cgtcttcgag ggctccttcc ggaagcgcgc ccccgacggg
      181 aaggtccacc tcatcgagga gcccgaggag cgctgagggg ggcacggacg gcgccgagga
      241 cgccggaggg ccccggcccc cacgaggccg gcgacgcggc gggcgttcac gaaggcgccc
      301 cggccgggcg gactccgggc gtggagggag agcgcccgac cgcgcgtccg agaggagggc
      361 ggcggcggcc cgctccggac gcgaccgtgc cgcacgacgc gacatccgga gggagaatcg
      421 caccatgacc gcagcgccat cgcccggcga agggatgctc gctccgctcc gcgacctgct
      481 gcccgagctg gaggccgcct acaaggacgt tcacgcgcat ccggagctct cgatgcagga
      541 gcaccggacg tcgcagctcg ccgccgaccg ccttcgcgcc gccgggttcg aggtggtcgc
      601 gggcgtcggc ggaacgggcg tcgtcgggct gctccgcaac ggctccgggc cgacggtgat
      661 gctccgggcc gacatggacg cccttccggt gcgggaggcc acgggccttc cctacgcgag
      721 ccgcgagacc gccgtcgatc gcgaggggaa ttccgtcccg gtcatgcacg cctgcgggca
      781 cgacatgcac gtggcctggc tcctcggcgc cgcggcgctc ttcgcgcgaa gccgcgacgc
      841 gtggcgcggg acgctgctcg ccgtcttcca gcccgccgag gagacgggcg agggagcacg
      901 ggcgatgatc gacgacgggc tgttcgggcg cttcccgaag ccggacgcga tcctcgcgca
      961 gcacgtgatg gtcggcccgg ccggccacct cagctcgcgg cccggcgtcg tcacctccgc
     1021 cggagacagc cttcaggtcc gcatgttcgg gcgcggcgcg cacggctcca tgccccaggc
     1081 gagcgtcgac cccgtggtcc tggcggcggc gacggtcctc cgcctccaga cgatcgtctc
     1141 gcgcgagatc gcggcgacgg acgcggccgt cgtgaccgtc gggtcgctgc aggcgggcac
     1201 gaaggagaac gtcatccccg acgaggccgt cctcaagctc aacgtccgga cgttcgacga
     1261 gagcgtccgc gcgcgggtgc tggcctcgat cgcgcggatc gtcgacgcgg aggcggcggc
     1321 ctccggcgcg ccgcggaagc cggagatcac cgtcctggac agctactcgc tcgtccggaa
     1381 cgaccccgag gcgacccggc gcgtcgccga cgccctccgg cagcacttcc ccgcggaccg
     1441 cgtccaggag gcccagccga cgacggccag cgaggacttc ggctgcttcg gctcggaatg
     1501 gcactgccct tcggtcttct ggttcgtggg cggcaccgac ccggacatct acgcccggga
     1561 gaaggcggac ggccgggtcg gagagatccc gaccaaccac aacccccggt tcgcgccggt
     1621 gatccacccc accctggaag cgggcgtcga ggcgatggtc gtcgcggcgc tggcatggct
     1681 cgccgcatga cgggcgtcga gcccgccctg gcgagcacgc tcctcgccgc cttcgacctg
     1741 gccgggactc tcgtcttcgc tctgagcgga gcgacgaagg ccgtccagca caggctcgac
     1801 ttcttcggcg tgatggtcct ctgcttcgcg gcgggcaact ccggcggcat cgcccgcgac
     1861 gtcatgatcg gcgccgttcc cccggccgcc ctcagccact gggggtacat cgcggtgtcc
     1921 ctcttcgcgg gcggggtcac cttcttctgg tatccgctga tcgatcgcct gcggagtccc
     1981 gtcctgctct tcgacgccgc cgggctggcc ctcttcgtcg tctccgggac cgacaaggcc
     2041 cttgcgtatc acgcgggccc cgtcgcggcc gtcctcttcg gaacgctgac ggggatcggc
     2101 ggcgggatgg tgcgggacgt gctcgtcggg gagactcccg cggtcctcaa gacggacgtc
     2161 tacgctctgg ccgctctcgt cggggcgggc gcgctggtgg ccggacggac gctgcacctc
     2221 cccgcgcccg tggcgtcgat cctgggcgcg accctctgct tcggcatccg cctgggcgcc
     2281 atgcgctgcg gctggcggct cccgtcggcc cgcccggacg gaccggcggc gacgggtcat
     2341 actcgggccc gacgtgaaga gcacgatcgg tgagacgccc cgaccggccg gcccgtggca
     2401 gcggagcgcg cgcgagctcc tcgaggagct cggctgcgcg aacggcgcgg gcctctcggc
     2461 ggcggaggcc gcggcgcggc tcgggcggtt cggcacgaac gagctgaccg agcgggccgg
     2521 ccggagccgg ctcgagatcc tccgcgagca gctcctgaac gtgatgacgg tggtcctcgt
     2581 cgtcgccgcg gccctctcgg ccctgctcgg ggactgggtc gaggcggtcg tcatcctcgc
     2641 gatcgtggtc ctgaacgccg ccctcgggta ctcgcaggag tatcgagccg agcggtcgat
     2701 ggcggctctg aagcggatgt cggtgccgac ggtgcgggcc cggcgcgacg gccgggtgca
     2761 ggaggtctcc tcccgggagc tcgttccggg tgacgtcgtc ctcctcgaga cgggcaacgt
     2821 cgtcccggcc gacggccgcg tcctttcgag cgtgaacctc cgggcgcagg aggccgcgct
     2881 cacgggagag tcggaggcgg tcgagaagga cgccgaggtc gtcctggagc cggagaggcc
     2941 gctcgccgag cgtcggaaca tggtcttcgc ggggacggtg gtcacctggg gccacggcga
     3001 ggccgtcgtg acggcgaccg ggatggcgac cgagctcggg aagatcgcga gcctcatcca
     3061 gtcggtcgtg gaggagaaga ccccccttca gcagcggctc gaccggatgg gaaagggact
     3121 cgcgatcggg gcgggcgtcc tcgtcgccgt cgtcttcctc ctcgggatcc ggtcggccgg
     3181 cacgcgcgag gaggtcttcg aggtcctcct gacggcggtg agcctcgcgg tcgccgcgat
     3241 tcccgaggcg atgacggccg tcgtgacgat cgccctctcc ctcggcgcgc agaggatgct
     3301 ccggcgcaga gcgctcatcc ggaagctccc ggccgtcgag acgctcggct cggtcggggt
     3361 catctgctcg gacaagacgg ggacgctcac gcagaaccgt atgaccgtga cggcgctcga
     3421 cgtggcggac caccggatcg acctcgtcca gcgcgagggc acggagcact tctcgctccg
     3481 gagggtcggg gccggcgagg ccgagccgga gctcgctccg acgctcgacc tcctcctcgt
     3541 cgggggcgcg ctctgcaacg acgccgcgct cgtgaaggag gaaggcgcgg acggggcgtt
     3601 ccacgccgtc ggggacccga cggagggttc cctcgtcctc gcggccgccg aatacggcgc
     3661 tcggaaggac gagctcgacc gggcgctccc gcgcgtcgcg gagctccagt tcgactccga
     3721 gcggaagcgg atgacgacgg tccaccggct ccccgccggc gacgcggagc tccctcccgc
     3781 cctccggccg ctctgggctc ggcgcgtcct tccggaggag acgccccgct tccttgccgt
     3841 gacgaagggg gcgatcgacg gcctcctggg aaagacgcgg agcgtctggt ccggggagtc
     3901 ggccgtcccc ctggacgacg aggcgcgccg gagggtgatg gccgcgcacg acgacctcgc
     3961 ggcgaagggg atgcgcgtcc tcggtctcgc cgtccggccg ctcggcggcc cgccctccgc
     4021 cgccgagacc gcctcgctgg aggaggagct tcttctcgtc ggcctcttcg ggatgctcga
     4081 cccggcacgg ccggaggtcg cggacgcggt ctcgcgctgc cgcgcggcgg ggatccggcc
     4141 gatcatgatc acgggggacc acccgctcac ggcgcgccac atcgcgaggc aggtcggcat
     4201 caccgagggg gacgagttcc ggacgggagc cgacatcgat cgcctctcgg acgcggagct
     4261 ggcccgggcc gtgggcgagg ttccggtctt cgcccgcgtc tcgccggagc acaagctccg
     4321 gctcgtcgcg agcctccagg cgcagggcca cgtcgtcgcg atgaccggcg acggggtgaa
     4381 cgacgccccc gcgctccgga aggccgacat cggcgtcgcg atggggatca ccggcaccga
     4441 cgtctcccgc gacgccgccg agatggtcct cctcgacgac aacttcgcca cgatcgtcgc
     4501 ggccgtcgag gaaggccgga agatctacga caacatccgg aagttcatca agtacctgct
     4561 cagctgcaac gcgagcgaga tcgcggtcat gctcctggcg ccgctcttcg gcatgccgct
     4621 tccgctcctg ccgctccaga tcctctggat gaacctcgtc acggacggtc tcccggcgct
     4681 ggcgctcggc gtggagccgg ccgaggccga catcatgaag cgaccgcccg cctcctcgga
     4741 ggagagcatc ttcgggaggg ggctcgtccc gttcgtcctc gtgatgggcg tcgtcatgag
     4801 cctgatctcg ctcgggatcg ggctttgggc ctaccggagc ggagacccgg cctggcagac
     4861 gctcctcttc acgacgctca tcttctcgca ggtgatcctg gcgctcgagg tgaggtccga
     4921 gaaggcctcg ctcctcacgc tcggcctcct gacgaacccg ctcatggtgg ccgccttcct
     4981 ctcgacggtc ggcctgcagg tcgccgtcgt ctacgccccg ttcctccagc gggtcttctc
     5041 gaccgcgccg ctcggcgcgc gcgacctcgg gatcgcgttc ggctccggcc tcgcggtcct
     5101 cctcgtcgtg gaggcctgga aggccgtccg gcggcggcgg ggcgggccct gacggacggg
     5161 gcggccgggt gcggcagaat cccggaatga cgcacccgtc ccgtcccccg aaccggctcg
     5221 cgagcgagcc gtccccctac ctgcgccagc acgcgcacaa cccggtcgac tggtatccgt
     5281 ggggggagga ggcgttcgag aaggcgcggc gggaggacag gccgctcttc ctctccatcg
     5341 gctattccgc ctgccactgg tgccacgtca tggagcacga gtcgttcgag gacgaggggg
     5401 tcgcagcgct cctgaacgag cggttcgtgt cggtgaaggt cgaccgcgag gagcggcccg
     5461 acgtcgacga cgtctacatg agcgcggtgc agacgatggt cgggcgaggc ggctggccgc
     5521 tctcggcgtt cctcttcccc gacggacgcc cgttcttcgg cgggacgtac ttcccgcgcg
     5581 aggaccggcg cggccaggcg gggttccgga cgatcctcga gaagctctcc gaggcctggc
     5641 gcgagcggcg cgaggagctc tcgaaggggg ccgacgagat cgtcgcggag gtggagcgcg
     5701 cgggccgcat caccgatcgc gccgggcgcg agccgctgac cgcgcggacg ggaagcgtcc
     5761 tgaccgagac gctcaagcgg acgttcgacg cccggcacgg cggcttcggc ggcgcgccga
     5821 agttcccgcc ccacctcgcg ctcgagtggc tcctcctgaa gggcgtcgag ggggactcga
     5881 tcgcccggtc gatggcgctg aagacgctcg acgagatggc cctcggcggg atccacgacc
     5941 acctcgccgg cggcttccac cgctactcga ccgacgagcg gtggctcctt ccgcacttcg
     6001 agaagatgct caccgacaac gcccagctcc tcgcgctcta cgcgcgggcg ttcgtcctga
     6061 cggggcgcga cctctactgg cggacggcgc gcgggatcgg ggactggctc ctttccgaga
     6121 tgcggggcga ggaggggggc ttcttcgccg cgaccgacgc cgactcggaa ggagaggagg
     6181 ggcgctactt cgtcttcacc gaggaggagg tccgctcggt cgccggagac gacgcggcgt
     6241 tcttcgcgtc cgcctaccgc gtccggcccg aggggaactt ccacgacgag tcgaccggcc
     6301 gcgcgacggg ccggaacgtc ctccacctcg ccggggagcc ctcgtccgag gaggagcggc
     6361 ggctcgcgcc gatccgcgcg cgcctgctcg agcggcggcg cctccgcgtc ccgccgggga
     6421 ccgacgacaa gcggatcgcg ggctggaacg ccctcgccgt ctccgggctc gccgtcgcct
     6481 cgtcgatcct cggcgagccg cgctacctcg acgccgcccg cgcctcggcg gagttcctcc
     6541 tgacgcggat gcgcgacgcc tcgggccggc tcttccggac gtggaaggag ggcgaggcgc
     6601 ggatcccggc gttcctcgag gacgaggcgt tcctcgccct cgcgctcctc gacctcgccg
     6661 aggccgagcc cgagccg
//
LOCUS       DAOTEZ010000225         6668 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_8205_length_6668_cov_6.245577, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000225 DAOTEZ010000000
VERSION     DAOTEZ010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6668
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8205_length_6668_cov_6.245577"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="P5164_12340"
     CDS             complement(<1..510)
                     /locus_tag="P5164_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44722.1"
                     /translation="MRSRVGLWLLALLLTLASAVFQRLTGPTHPVRGAVTLGGTEVRL
                     RLLRSHGGEGDQPVVVRVADRAVSGSVAWRRYPTNDAFAVLPMARDGETLRAALPHQP
                     PAGKLEYQVRLARGGETALFPERPAITRFKGAVSPFVLVPHIVAMFGGMLVANAAGLL
                     ALRGEKRLLG"
     gene            565..1446
                     /locus_tag="P5164_12345"
     CDS             565..1446
                     /locus_tag="P5164_12345"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRY44723.1"
                     /translation="MPVSRGLRAALLVSTLLAAGSAPGSPKAPERGAFVSGPWLEAQL
                     PPAVVLLDARPFREFLSGHLPGAQSVSPENLRSTAGGVPAALYPPEILDAIFARVGID
                     AKAHVVVYGAETDVDATYVATAARIAGAARVEVLDGGFTRWTAEGRPVTRERVLSPGV
                     KPDRGIDARLLVSGAEARKRVGDGKTVFLDVRPAEQWLAGRIPGAKNRFWKADVADGS
                     LRPLADLRDELEAAGVSWERPVVVYCNSGHQASEALWLLREALGHPDARLYQGSWLEW
                     SMTPGAPRESGPLAETP"
     gene            1443..2216
                     /locus_tag="P5164_12350"
     CDS             1443..2216
                     /locus_tag="P5164_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRY44724.1"
                     /translation="MTPGEIAALLAASAGAGVMNALAGGGTILTFPTLVLLGIPAIQA
                     NATSTVALLPGAAASMAGYRREVATHAAWLKTLFLPSLVGGAIGSVLLLKTPEKLFAS
                     LAPFLVLFATLLFILQIVLAKRGGDAEVHAPHGPTNARWGVATLAQLAVAIYGGYFGA
                     GIGILMLVVLGFLGLTDIHAMNGLKNFFGITINLVAAAWFIWNGAVLWPIALVMVAGA
                     TVGGYAGARFARRIGKEKARAAVVVIGLGVTLVLLLQRA"
     gene            complement(2185..4758)
                     /locus_tag="P5164_12355"
     CDS             complement(2185..4758)
                     /locus_tag="P5164_12355"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRY44725.1"
                     /translation="MRGVLGTGGSAVVYEAFDRELKRSVALKVLRTDRTSEAALKRFR
                     REVAIARDAASPRLVRVFDIGQAGDTVFLTMELVEGESLRELLARGPLLPERATAIAA
                     EVLRALADLHALGIVHRDVKPGNILLSDAGEVKLADFGLARHWEGSETRVTETEALVG
                     TVEYLSPEQALGDLLDARSDLYAVGVVLFEMLAGRVPLRGESAIGTIVAHIRQEPIDV
                     RKLRPEAPAWLSAVVARLLAKDRERRYATAADVLADLEARRARRAPRRLGRALALGGS
                     ASLVALLLTLAFVPVFPWNRPRLTRLAPDGAGGVQALDGKGRVLWRRADTAAGRQIAL
                     ARLAGGETGAIAVPGGTGDPRKRVVVLDGLTGRPLADLELPDPAGDPDGAGAFFGFSS
                     TFAVGAVFPFDEDGDGDDEVAVSLAHIPLWAGATYVCDPGRRRVVVAFYGSGHHYARA
                     AVDVDVDGDGRKELILAGLNNRMGYSAAVAALRIPGEGEGRRAWSELPFAESPDRPGW
                     RDEKSLLAWYALVPDAAFGRVEGQVLEASAGLLRVGAWGPQQVELALDGFPRGPREPG
                     ESQRRSEAREEAYALLREGMRLRGAGLAREALAAVEQALEKARAAGDAPLGQWIARAR
                     ARALLDTGQPGEADTAWGASFERSVEPARIALEAGRAFHLRGDLARAVAWYRRGLTGR
                     GDYARIGIYPRDLMLGSVLALGEAGRWDDTAAEVDRFEAAYPDRVRDVAAVRAFVAWR
                     SGRSPARFADSVADIGLFRYWGLEARRASGEAATPLLADVERVGREAPDVAPLFASLR
                     AELLAALGRKDEARALAAEALDGARDLAATDTGVRAHLSVVEARARSLGVRPAGEGAP
                     G"
     gene            complement(4966..5730)
                     /locus_tag="P5164_12360"
     CDS             complement(4966..5730)
                     /locus_tag="P5164_12360"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY44726.1"
                     /translation="MTDADPKDPYARLSYRRLIAWPARIEREAPFLRKVLAGAPSRRL
                     LDLGCGTGEHARFLQAEGFAVTGVDASEAQLAQAREAGPGPEFVKGDLAELGAAVAGP
                     FGGAISLGNGLPHLHAPEMSRFLVHLAALLLPGAPVLFQLLNYDRILDEGVTSLPLNV
                     RDDGGERVVFLRLMEPRPDGTVLFNPTSLRWRPGAEPPVEVVAAKNVLLKGWRRAELE
                     GALGEAGLRVETVFGGMKGESWEARGSADTVVLARR"
     gene            complement(5851..6645)
                     /locus_tag="P5164_12365"
     CDS             complement(5851..6645)
                     /locus_tag="P5164_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005032018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HRY44727.1"
                     /translation="MSGDKDIDIKVRIWRQKSPSDKGGLVTYDVPGVSTGSSFLEMLD
                     LVSERLMEKGEEPIALDHDCREGICGACSLVINGVPHGPRSATTTCQLHMRSFQDGQT
                     ITVEPFRAKAFPVIKDLIVDRSAYDRIIAAGGFVSVNTGVGVDANAIPVPKENAELAM
                     DAAACIGCGACAAACPNASAMLFVGAKVSHLGLLPQGQPERKERVLKMVEQMDAEGFG
                     GCTNMGECEAACPKEIKLEVIARLNRDYLGAKVTKRPKGAEKGGGA"
BASE COUNT          857 a         2578 c         2366 g          867 t
ORIGIN      
        1 gccgaggagg cgcttctcac cgcgcagggc gagaagcccc gcggcgttcg cgacgagcat
       61 ccccccgaac atcgcgacga tgtgcgggac gagaacgaac ggcgagaccg cccccttgaa
      121 gcgggtgatc gccgggcgct cggggaagag cgccgtctcg ccgccgcggg cgaggcgcac
      181 ctggtactcg agcttgccgg ccggtggctg gtgcgggagc gccgcccgga gcgtctcccc
      241 gtcccgggcc atcggaagga cggcgaacgc gtcgttcgtc gggtagcgcc gccacgcgac
      301 gctgccgctg acggcccggt cggcaacccg cacgacgacc ggctggtccc cttcgcctcc
      361 gtgcgagcgg aggaggcgga ggcgaacctc ggtcccgccg agcgtcaccg cgccgcggac
      421 ggggtgggtc ggcccggtca gccgctgaaa gactgccgac gcgagggtga ggaggagggc
      481 gaggagccag agtccgacgc gactgcgcat gcggcgcgaa ggatagcccg gcagctgggg
      541 catcattccg gtccggaggt cccgatgccc gtctcccgag gcctgcgagc cgccctgctc
      601 gtctcgacgc tcctggcggc tggctccgcg cccgggtcgc cgaaggcgcc cgagagggga
      661 gccttcgtct ccggcccgtg gctcgaggcg cagctccctc ccgccgtcgt cctcctcgac
      721 gcgaggccct tccgcgagtt cctctccggg cacctccccg gagcgcagag cgtctccccc
      781 gagaacctcc gctccaccgc gggcggcgtc ccggcggcgc tctacccgcc cgagatcctc
      841 gacgcgatct tcgcgcgcgt cggaatcgac gcgaaggccc acgtcgtcgt gtacggagcc
      901 gagaccgacg tcgacgccac gtacgtcgcg acggccgcgc ggatcgccgg ggccgcccgc
      961 gtcgaggtcc tcgacggcgg cttcacgcgc tggacggcgg agggccggcc cgtgacgcgc
     1021 gagcgggtcc tctcgcccgg ggtgaagccg gaccgcggga tcgacgcccg cctcctcgtt
     1081 tcgggcgccg aggcccggaa acgcgtcgga gacgggaaga ccgtcttcct cgacgtccgg
     1141 ccggcggagc agtggctcgc cggccggatt cccggcgcga agaaccgctt ctggaaggcg
     1201 gacgtcgcgg acggctcgct gcggccgctc gccgacctcc gcgacgagct cgaggcggcc
     1261 ggcgtctcgt gggagaggcc cgtcgtcgtc tactgcaaca gcgggcacca ggcgtccgag
     1321 gcgctctggc tcctccgcga ggcgctcggg cacccggacg cccggctcta ccagggctcc
     1381 tggctcgagt ggtcgatgac gcccggcgcg ccccgggaga gcggcccgct ggcggagacg
     1441 ccgtgacccc tggcgagatc gcggcgctcc tcgcggcgtc cgccggcgcc ggagtgatga
     1501 acgccctggc cgggggcggc acgatcctca cgttcccgac gctcgttctc ctcggcatcc
     1561 ccgccatcca ggcgaatgcc acctccaccg tcgccctcct ccccggagcc gccgcgagca
     1621 tggcgggcta ccggcgcgag gtggcgacgc acgcagcctg gctcaagacg ctcttcctcc
     1681 cgagcctcgt cggcggcgcg atcgggtcgg tcctcctcct gaagaccccg gagaagctct
     1741 tcgcgagcct cgcgccgttc ctcgtcctct tcgcgacgct cctcttcatc ctccagatcg
     1801 tcctggcgaa gcggggcggc gacgcggagg tgcacgcgcc gcacggaccg acgaacgccc
     1861 gctggggcgt cgcgacgctc gcgcagctcg ccgtcgcgat ctacggcggc tacttcggcg
     1921 ccggcatcgg catcctgatg ctcgtcgtcc tcggcttcct cggcctcacc gacatccacg
     1981 cgatgaacgg cctgaagaac ttcttcggga tcacgatcaa cctcgtcgcg gcggcctggt
     2041 tcatctggaa cggcgccgtc ctctggccga tcgctctcgt catggtcgcg ggcgcgaccg
     2101 tgggcggcta cgcgggggcg cgcttcgccc ggcggatcgg gaaggagaag gcgcgggcgg
     2161 ccgtcgtcgt catcggcctc ggcgtcaccc tggtgctcct tctccagcgg gcctgacgcc
     2221 gagactcctc gcgcgcgcct ccacgacgga gaggtgcgcc ctcacccccg tatcggtcgc
     2281 agcgaggtcc cgggcgccgt cgagcgcttc cgcggccagg gcgcgcgcct cgtccttccg
     2341 cccgagcgcg gcgaggagct ccgcgcgaag cgaggcgaag agcggcgcga cgtcgggggc
     2401 ttcgcgtccg acgcgttcga cgtcggcgag aaggggggtc gcggcctccc ccgacgcacg
     2461 ccgcgcctcg aggccccagt accggaagag cccgatgtcg gcgacgctgt cggcgaagcg
     2521 cgccggactg cgtccgctcc gccaggcgac gaaggcccgg accgcggcca cgtcccggac
     2581 gcgatcgggg taggccgcct cgaaacgatc gacctcggcc gccgtgtcgt cccatcgccc
     2641 cgcctcgccg agcgcgagga cgctccccag catcaggtcg cgcgggtaga tcccgatccg
     2701 ggcgtagtcg ccgcgccccg tcaggccccg gcgataccag gcgaccgccc gggcgaggtc
     2761 cccccggagg tggaacgcgc gccccgcttc gagcgcgatc cgcgccggct cgaccgacct
     2821 ctcgaacgag gccccccagg ccgtgtcggc ctcccccggc tgccccgtgt ccaggagggc
     2881 acgggcccgc gcgcgcgcga tccactgccc cagcggggcg tctcctgccg cgcgagcctt
     2941 ctcgagcgcc tgctccacgg ccgcgagcgc ttcccgcgcg agccccgccc cgcgcagccg
     3001 catcccctcg cggaggagcg cgtacgcctc ctcccgcgcc tcggagcgcc gttgcgactc
     3061 gccgggctcg cgcggcccgc gcggaaagcc gtcgagcgcg agctccacct gctgcgggcc
     3121 ccacgctccc acgcgcagga gcccggccga cgcctcgagg acctgcccct cgacgcgacc
     3181 gaaggcggcg tccgggacga gggcgtacca ggcgaggagc gacttctcgt cccgccagcc
     3241 cgggcgatcg ggcgactcgg caaacgggag ctccgaccag gctcgccttc cttccccttc
     3301 ccccgggatc cggagcgcgg cgaccgcggc agagtagccc atccggttgt tgaggccggc
     3361 caggatcagc tccttccggc cgtcgccgtc gacgtcgacg tcgacggcgg ctcgcgcgta
     3421 gtggtggccg gagccgtaga acgccacgac gacgcgccgc cggcccgggt cgcagacgta
     3481 cgtcgccccc gcccagagcg ggatatgggc gaggctcacg gcgacctcgt cgtcaccgtc
     3541 gccgtcctcg tcgaaaggga acacggctcc gacggcgaac gtgctcgaga atccgaagaa
     3601 tgcccccgcc ccgtctgggt cgccggccgg gtcggggagc tccaggtcgg cgaggggacg
     3661 gccggtgagg ccgtcgagga cgacgacgcg cttcctcgga tcgcccgtcc cgcccggcac
     3721 cgcaatggcc cccgtctcgc cgccggcaag cctcgcgagc gcgatctggc ggcccgccgc
     3781 cgtgtccgcc cgcctccaga ggacgcgacc cttcccgtcg agcgcctgca cccctcccgc
     3841 gccgtcgggg gcgaggcgcg tcaggcgtgg ccggttccac gggaagaccg ggacgaacgc
     3901 gagggtgagg aggagggcga cgagcgatgc cgagcccccg agcgcgaggg ccctcccgag
     3961 ccggcgcggc gcgcgccgag cgcggcgcgc ctcgaggtcg gcgaggacgt ccgcggccgt
     4021 cgcgtaccgc cgctcgcggt ccttcgcgag gagacgggcc acgacggccg aaagccacgc
     4081 gggcgcctcc gggcggagct tcctcacgtc gatcggctcc tgccggatgt gcgccacgat
     4141 cgtcccgatg gccgactcgc cgcgcagagg gacccgcccc gcgagcatct cgaagaggac
     4201 cacgccgacc gcgtagaggt cgctccgcgc gtcgagcagg tcgccgagcg cctgctccgg
     4261 cgagaggtac tcgaccgtcc cgacgagcgc ctccgtctcg gtcacccgcg tctcgctccc
     4321 ctcccagtgg cgggcgaggc cgaagtcggc gagcttcacc tctccggcgt ccgagagaag
     4381 gatgttcccg ggcttcacgt cgcggtggac gatcccgagg gcgtgaaggt cggcgagggc
     4441 gcggagcacc tccgccgcga tcgcggtcgc ccgctccggc agcagcggac cgcgcgcgag
     4501 gagctcgcgg agcgactccc cttccacgag ctccatcgtc aggaagaccg tgtcgcccgc
     4561 ctggccgatg tcgaagacgc ggacgaggcg cgggctcgcc gcgtcgcgcg cgatcgccac
     4621 ctcgcgccgg aagcgcttca gcgccgcctc cgacgtccgg tcggtccgca ggaccttcag
     4681 cgcgacgctc cgcttcagct cccggtcgaa cgcctcgtag acgaccgccg agcctcccgt
     4741 gccgaggacg ccgcgcacct cgtatcgccg gccgatcgcc gtgcccggcg cgaagaggcg
     4801 gacgggcaga cgcggactct cgcccgcatc cgccgcggaa tcgccccggt cgggtcgttc
     4861 gtcgtcgagc gtacggagtc tctccgaccg gagatcatag gacggccgga gcccgctcgt
     4921 cccgcagcag gagctcgccc ggcgtgcgaa cgccgccgcc tttcctcagc gccgggcgag
     4981 gaccaccgtg tcggccgagc ccctcgcctc ccaggactct cccttcatcc ctccgaagac
     5041 cgtctccacc cggagcccgg cttccccgag cgctccttcc agctcggccc gccgccaccc
     5101 cttcaggagg acgttcttcg ccgcgacgac ctccaccggc ggctccgcgc ccggccgcca
     5161 ccggagcgac gtcgggttga agaggaccgt cccgtcggga cgcggctcca tcaggcggag
     5221 gaagacgacc cgctcgcccc cgtcgtcccg gacgttcagc ggcaggctcg tcaccccctc
     5281 gtcgaggatc cggtcgtagt tgaggagctg gaagaggacc ggtgcgccgg ggaggaggag
     5341 cgccgcgagg tgcacgagga agcgggacat ctcgggcgcg tgaaggtgcg gcaggccgtt
     5401 cccgagcgaa atcgctcccc cgaacggccc cgccaccgcc gcgccgagct ccgcgaggtc
     5461 tcccttcacg aactccggcc ccggccccgc ctcgcgggcc tgcgcgagct gcgcctccga
     5521 ggcgtcgacg cccgtcaccg cgaatccctc cgcctggagg aaccgcgcgt gctcgcccgt
     5581 gccgcagccg aggtcgagga gccgccgcga cggcgcgccc gcgagcacct tgcggaggaa
     5641 cggcgcctcg cgctcgatgc gcgcgggcca ggcgatgagc cggcggtagg agagccgggc
     5701 gtacgggtcc ttcgggtcgg cgtccgtcat gccgggagtc tcgcaccggc gcgcccccga
     5761 aagcgcgcgg ccggggtcgc ccccggccgc gccgatcttc cgttccgtgg accgggtgcg
     5821 cgaagcgccg tcgcggcgcc ccgccgctcc tcaggcgcct ccgcccttct cggccccctt
     5881 gggccgcttc gtgacctttg caccgaggta gtcgcggttc aggcgcgcga tgacctcgag
     5941 cttgatctcc ttcgggcagg cggcctcgca ctcgcccatg ttcgtgcagc cgccgaaccc
     6001 ctcggcgtcc atctgctcga ccatcttcag gacgcgctcc ttccgctcgg gctgcccctg
     6061 cgggaggagg ccgaggtgcg agaccttcgc cccgacgaaa agcatcgccg aggcgttcgg
     6121 gcaggcggcg gcacaggcgc cgcagccgat gcacgccgcc gcgtccatcg cgagctcggc
     6181 gttctccttc gggaccggga tggcgttcgc gtcgacgccg acgcccgtgt tcaccgagac
     6241 gaagccgccc gccgcgatga tccggtcgta ggccgaccgg tcgacgatca ggtccttgat
     6301 cacggggaag gccttcgccc ggaacggctc gacggtgatc gtctggccgt cctggaagct
     6361 cctcatgtgg agctggcagg tcgtcgtcgc ggagcgcggc ccgtgcggga cgccgttgat
     6421 gacgagcgag caggcgccgc agatcccctc gcggcagtcg tggtcgaggg cgatcggctc
     6481 ctcgcccttc tccatgagcc gctcgctgac gaggtcgagc atctcgagga acgaggagcc
     6541 ggtgctgacg cccgggacgt cgtacgtgac gaggccgcct ttgtcggagg gcgacttctg
     6601 gcgccagatg cggaccttga tgtcgatgtc cttgtcgccg ctcattactt gtagctcctc
     6661 gtggcgag
//
LOCUS       DAOTEZ010000226         6648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_8239_length_6648_cov_5.073563, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000226 DAOTEZ010000000
VERSION     DAOTEZ010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6648
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8239_length_6648_cov_5.073563"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4163
                     /locus_tag="P5164_12370"
     CDS             <1..4163
                     /locus_tag="P5164_12370"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRY44728.1"
                     /translation="NCELAPTPAEGTLSVEKVPASGPTVAVPDNGTVFVGDELRIRPA
                     FAPSDTLQPVTDWRFDYAFHEGVPTDASGATPFLVNPDVPFTPGTFPSELKLIGPCDP
                     SQDGDQNVSSGTGCWDSVLASGDFAASDPAAGTVKQIPLAFEVRNALNGNGSDLTKHR
                     INWKVPKPLLKSASILSGESVEDGSEGTPARTSNAYRWYFSTTPVGEAGSDVLTLKAD
                     CTGPVCNPGLAQRGTYRYWVSVPYRSGFRTPDCPGLDGTSCTVPTAPTVAVTDVVLGF
                     SVPESGNVGAQALTIASSSKKGTAATPCGGTSGFIYDLCTVSGGDCPEGSYAGVGFGA
                     TNPFPAAGTGTVSIPMPQEGTYGVRIRYRYTTTGDCGNGNSAALTATWPDQGQWAPLT
                     VIRSVPTVRLFSNSTGTTELSKWAGTYSLTTGQQAYVYAYVNDVREAALPAGASIIHT
                     NTETGQVTQIGTKQGAGFSVATRGDYVVTLRGYGVDATAAIAVEAPLGGGGGGGGTAP
                     QALAISSIWANPSSVAVGQAVTISCSATGGTGPYSYTFSFGDGTPNETNASGNVAHAF
                     SEAGTKTVRCTATDSANANRSASVYVTVGTSAGSCDFVINDSTGAKLSFDPFSRSYDA
                     ISGQPLTFVASNVTGTASWTFRPGATATGNPVTYTYTITGLEMQAFTATLTATGCSKS
                     YPVNVSPSSGQVFLDFAVADADTGTALARSGTVFGPATAGQRLKFTPSGATGDVSWVF
                     GDGTSSAEASPVKVYTPAVDASYTVTLTNGAAQKQYTVSVKGSTGAPLTGNYTYQYAD
                     GTSVNRSAVEPNKPIRFTGADQATTYTWDFGDGTPLGQGSPKTYSFTRGGTFSVKLTV
                     ARNGVPGTVTTASPTVFTVKPPPDPLLWVAGGMAYTDNGSGMKWQSDLSIHNPGTTTA
                     TVSLAYVAGATWDGATKVTWIPQALVAGETKSYTNVLKSLFLLSEGAWGVVLVRGDDI
                     SVPPVIVSRTYNAANADEVGTFGLSVPALSVAGGVQPQSAGGGNVLAGLRHDGVFRTN
                     LAIANLKGETAEVEVVFRDVNGNVLGVPALVTVEARGVKQIGSILSADPAQSGAAMGG
                     AGYGSPASDFSAEVKLRKGSGIYPYATVIDNGTGDSIVVTPAARPSPHYRLPGVVRVQ
                     GKNGPFWMSDVAVLNPSAKERRVRITYSYKKVGAAGRATMENTLVFKPWEMKIAVDFV
                     KTWLGLAENDAAGYADSFVDIAPAADDAAPTENLVVVGKTYMTTAKGTIGLQLDGFAY
                     EDGLSAQGSRQRVVLSGLEANRDYRTNLALFLTPGSTGSAQVDVRVLDSFGRESKKIS
                     SVGLNGDAPFVQIGNDSLFAGLTTGEMSRATIVIDNPRGTAVVGAYATVIDNRSEDAT
                     FVAGQPAP"
     gene            complement(4265..5782)
                     /locus_tag="P5164_12375"
     CDS             complement(4265..5782)
                     /locus_tag="P5164_12375"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HRY44729.1"
                     /translation="MIRLYTVVFLSGAALMGLEIAGSRVMAPVFGTSIFVWGSLITTF
                     LASLAAGYALGGHLADRRPRASLLGGILVGAGLLVWLLLARPAPLLALCASAPFPDRF
                     QALLAALLLFALPSVLMGAVSPFATRLAAREVGSIGRTAGSLAALSTAGSIVGTFSMT
                     FLLIPAFPIEPILFDVGVTLVLCGAFVSRDGLPARLGVAAAGLVAAAAVFLVRPERLP
                     PPVPDGTVVLRKETAYHRLLVVDQGPRRVLYFNNLAQGFVNRSTGMAAGRIYSDGLAA
                     SLLFRKAPARNAFVIGLGTGMLPRLFSEKAPGIAMTSVEIDPEVVRVAGEYFGFRPDA
                     NDRVLVGDGRAVLEREQGPWDAIILDAYFSDSVPFHLMTREFLELCRERLSPDGVFSA
                     NFVGTLMGQDQRLFWSFVRTAREVFPTVAILSSELSSGKRAFQSNAILVASSSRDRLS
                     KERVVAEGDRLAAALHHPPVAEWAREFHEGELRTEGVPILTDSFSPTDALQHLGR"
     gene            6091..>6648
                     /locus_tag="P5164_12380"
     CDS             6091..>6648
                     /locus_tag="P5164_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRY44730.1"
                     /translation="MPSAIRIVKDDPTASLVDFFENRSVGAALRQVVAPRNVRQQEYL
                     QAIGERDVVFAIGPAGTGKTYLAVAMAAAALLEKRVKRIVLCRPAVEAGEKLGFLPGD
                     LAEKVNPYLRPLYDALYDILGYEKVGRLLERQVIEIAPLAFMRGRTLNDAFIILDEAQ
                     NTTSEQMKMFLTRIGFGAKAVITGDI"
BASE COUNT         1125 a         2316 c         2316 g          891 t
ORIGIN      
        1 tgaactgcga gctggccccg acgcccgccg aagggacgct ctccgtcgag aaggtccccg
       61 catccggccc gaccgtggcc gtacccgaca acgggaccgt gttcgtcggc gacgagctcc
      121 ggatccggcc agcgttcgct ccgtccgata ctctccagcc cgtcacggac tggcgattcg
      181 actacgcgtt ccacgaaggg gtcccgaccg acgcgagcgg cgcgacgccg ttcctcgtga
      241 accccgacgt gccgttcacg cccggcacgt ttccgtcgga gctgaagctg atcggcccct
      301 gcgacccgtc ccaggacggc gaccagaacg tctccagcgg aaccggatgc tgggactccg
      361 ttctcgcgag cggcgacttc gccgcttccg accccgcggc cggcacggtc aagcagatcc
      421 cgctcgcctt cgaggtccga aacgcgctga acggaaacgg ctccgacctc acgaagcacc
      481 gtatcaactg gaaagtcccg aagccgctcc tcaagagcgc ttcgatcctc tcgggcgagt
      541 cggtcgagga cggctccgag gggacgccgg cccggacgag caacgcctac cggtggtact
      601 tctccacgac acccgtgggg gaggccggga gcgacgtcct gaccctgaaa gccgactgca
      661 cgggcccggt ctgcaatccc ggtctcgcgc agcgcgggac gtaccgctac tgggtctccg
      721 tcccctaccg gagcggtttc cgcacgcccg actgcccggg cctcgacggg accagctgta
      781 cggtcccgac cgcgccgacc gtcgccgtca ccgacgtcgt cctcggcttc tcggtcccgg
      841 agagcgggaa cgtcggcgcg caggcgctga cgatcgccag ctcgtcgaag aaggggaccg
      901 ccgcgacgcc gtgcggcggg accagcggct tcatctacga cctctgcacg gtcagcggag
      961 gagactgccc cgaggggagc tatgccgggg tcggcttcgg cgcgacgaat ccgttcccgg
     1021 ccgccgggac cgggaccgta tcgatcccga tgcctcagga ggggacctac ggggtccgga
     1081 tccgctaccg ctacacgacg accggcgact gcgggaacgg gaactcggct gcgttgaccg
     1141 cgacctggcc cgaccagggg cagtgggcgc cgctcacggt catccggtcg gtgccgaccg
     1201 tccggctctt cagcaactcg acggggacga cggaactctc caagtgggcg gggacctact
     1261 cactcacgac cgggcagcag gcgtatgtct acgcctacgt caacgacgtc cgcgaggccg
     1321 cgctcccggc gggcgcctcg atcatccaca cgaacacgga gaccgggcag gtcacgcaga
     1381 tcggtacgaa gcagggcgcg ggcttctcgg tcgcgacgcg cggcgactac gtcgtcaccc
     1441 tccggggcta cggcgtcgac gcgacggcgg ccatcgccgt cgaagcgccc ctcggcggcg
     1501 gcggcggcgg cggcgggacg gccccgcagg ccctcgcgat ctcctcgatc tgggcgaacc
     1561 cgagctccgt cgccgtcggc caggccgtca ccatctcgtg cagcgcgacc ggcgggaccg
     1621 gaccctacag ctacactttc tcgttcggag acgggacgcc gaacgagacg aacgcctcgg
     1681 gcaacgtcgc ccatgcgttc tccgaggcgg ggacgaagac ggttcgatgc acggcgacgg
     1741 acagcgcgaa cgcgaaccgc tcggcgagcg tctacgtcac cgtcgggacg agcgccggca
     1801 gctgtgactt cgtcatcaac gacagcaccg gggcgaagct ctccttcgac ccgttctcca
     1861 ggtcctacga cgcgatcagc ggccagccgc tcacgttcgt cgcctcgaac gtgacgggga
     1921 cggcgagctg gacgttccgc cccggcgcga cggcgaccgg caacccggtg acctacacct
     1981 acacgatcac cggcctcgag atgcaggcct tcacggccac cctcacggcc accggctgct
     2041 cgaagagcta tccggtgaac gtctcccctt cgagcggaca ggtcttcctc gacttcgccg
     2101 tggcggacgc cgacaccggc accgcgctgg cgcgctcggg gacggtcttc gggcccgcca
     2161 cggcggggca gcgcctcaag ttcacgccga gcggcgccac gggagacgtc tcctgggtct
     2221 tcggcgacgg gacctcctcc gcggaggcga gcccggtcaa ggtctacacc ccggccgtcg
     2281 acgcgagcta cacggtcacg ctcacgaacg gcgcggcaca gaagcagtac accgtcagcg
     2341 tgaagggatc gaccggcgca ccgctcaccg gcaactacac ctaccagtac gccgacggga
     2401 cgagcgtgaa ccgttccgcc gtcgagccga acaagccgat ccgcttcacc ggcgccgacc
     2461 aggcgacgac gtacacctgg gacttcggcg acggcacgcc gctcgggcag ggctccccga
     2521 agacctactc gttcacgagg ggcgggacgt tctccgtgaa gctcacggtg gcgcgaaacg
     2581 gggtgcccgg caccgtcacg acggcctctc cgacggtctt caccgtgaag ccgccccccg
     2641 acccgctcct ctgggtcgcg ggcggcatgg cctacaccga caacggctcg ggaatgaagt
     2701 ggcagtccga tctctcgatc cacaaccccg ggacgacgac ggccacggtc tccctcgcct
     2761 acgttgccgg cgccacctgg gacggcgcca cgaaggtgac ctggatccct caggcgctcg
     2821 tcgccggcga gacgaagtcg tacacgaacg tcctgaagag cctcttcctg ctcagcgagg
     2881 gcgcctgggg cgtcgtcctc gtccgcggcg acgacatctc ggtgccgccc gtcatcgtga
     2941 gccggaccta caacgccgcg aatgccgacg aggtcggcac gttcggcctc tccgttccgg
     3001 cgctctcggt cgccgggggc gtgcagcccc agtccgccgg gggagggaac gtcctcgcgg
     3061 gcctgcggca cgacggcgtg ttccggacga acctcgcgat cgcgaacctg aagggggaga
     3121 ccgcggaggt cgaggtcgtc ttccgcgacg tgaacgggaa cgtgctcggc gtgccggcgc
     3181 tggtcacggt cgaggcgcgg ggcgtcaagc agatcggctc gatcctttcg gccgatccgg
     3241 cccagagcgg ggcggcgatg ggcggcgcgg ggtacggctc gccggcgtcg gacttctcgg
     3301 ccgaggtgaa gctccgtaag ggctcgggca tctatccgta cgcgaccgtc atcgacaacg
     3361 gcacgggcga ctcgatcgtc gtcacgccgg cggcgcgtcc ctcgcctcac tacaggcttc
     3421 cgggcgtcgt tcgggtccag gggaagaacg ggccgttctg gatgagcgac gtcgcggtcc
     3481 tcaacccgag cgcgaaggag cggcgcgtcc ggatcaccta ctcctacaag aaggtcggcg
     3541 ccgccgggcg agccacgatg gagaacacgc tcgtcttcaa gccgtgggag atgaagatcg
     3601 ccgtcgactt cgtgaagacc tggctcggcc tcgcggagaa cgacgcggcc ggctacgccg
     3661 attccttcgt cgacatcgcg ccggccgcgg acgacgccgc tccgacggag aacctcgtcg
     3721 tcgtcggcaa gacctacatg acgacggcga aggggacgat cggcctgcag ctcgacggat
     3781 tcgcgtacga ggacgggctc agcgcgcagg ggtcccgtca gcgcgtcgtc ctctccggcc
     3841 tcgaagcgaa cagggactat cggacgaacc tcgcgctctt cctgactccc ggctccacgg
     3901 gcagcgccca ggtggacgtc cgcgtgctcg actccttcgg ccgcgagtcg aagaagatct
     3961 cttccgtcgg cctgaacggc gacgcgccgt tcgtgcagat cggcaacgac agcctcttcg
     4021 cgggcctgac gaccggcgag atgtctcgag ccacgatcgt gatcgacaac ccccgcggca
     4081 cggccgtcgt cggggcctat gcgaccgtga tcgacaacag gtccgaggac gcgacgttcg
     4141 tcgcgggcca gccggcgccc tgagtccggc ctgagataag acccccaagc ccgaaaccgt
     4201 cgggctcacc cctcacatcg gcccgggctc ccgcccgggc caattttttc gtcggtgccc
     4261 ggattcagcg cccgaggtgc tggagcgcat ccgtcgggct gaaggaatcg gtgaggatcg
     4321 ggactccctc ggtccgcagc tcgccctcgt ggaattcgcg ggcccactcg gcgaccggag
     4381 gatggtgcag ggccgcggcg aggcgatccc cctcggcgac gacgcgctcc ttcgagaggc
     4441 ggtcgcggga ggaggaggcg acgaggatcg cgttgctctg gaatgcccgt ttgccggagg
     4501 agagctccga ggagaggatc gcgaccgtcg ggaagacctc ccgggcggtc cggacgaacg
     4561 accagaagag ccgctggtcc tggcccatga gcgtcccgac gaagttcgcg ctgaagacgc
     4621 cgtccggcga gagacgctcc cggcagagct cgaggaattc ccgcgtcatc aggtggaacg
     4681 gaacgctgtc ggagaagtag gcgtcgagaa tgatcgcgtc ccacggtccc tgctccctct
     4741 cgagcactgc gcggccgtcg ccgacgagga cgcggtcgtt ggcgtcgggg cgaaagccga
     4801 aatactcgcc ggcgacgcgg acgacctcgg gatcgatctc gaccgaggtc atggcgatcc
     4861 cgggcgcctt ctcggagaag aggcggggga gcatcccggt accgaggccg atgacgaagg
     4921 cattccgggc cggcgccttc cgaaagagga gggaggcggc gaggccgtcc gagtagatcc
     4981 ggccggccgc catccccgtg ctccggttca cgaacccctg ggcgaggttg ttgaagtaga
     5041 ggacgcgccg gggaccctgg tcgacgacga ggagccggtg gtaagccgtc tccttgcgca
     5101 ggacgacggt gccgtccggt acgggcggcg ggaggcgctc cgggcggacg aggaagaccg
     5161 cggccgcagc gacgaggccg gcggcggcga cgccgagccg cgcggggagt ccgtcccggg
     5221 agacgaaggc gccgcagagg acgagcgtca ccccgacgtc gaagaggatc ggctcgatcg
     5281 ggaacgcggg aatcaggagg aacgtcatcg agaaggtgcc gacgatcgac ccggctgtcg
     5341 acagggcggc cagggaaccg gccgtccggc cgatggagcc gacctcgcgc gcggccaggc
     5401 gcgtcgcgaa cggggagacg gcccccatca gaacgctcgg aagcgcgaag aggaggagcg
     5461 cggcgaggag cgcctggaaa cggtcgggga acggcgcgga cgcgcagaga gccaggagag
     5521 gcgcggggcg ggcaaggagg agccagacga gaagcccggc tccgacgagg atcccgccga
     5581 ggagcgacgc gcgagggcgg cggtcggcga ggtgaccccc gagcgcatac ccggcggcaa
     5641 gcgacgcgag gaacgtcgtg atgagcgagc cccagacgaa gatcgaggtg ccgaagacgg
     5701 gcgccatcac gcggctgccg gcgatctcga ggcccatcaa ggcggcgccg gagagaaaga
     5761 cgacggtgta gaggcggatc acccccggat tttagggcgg gggaagcatt tgaccggtcg
     5821 tctcgcccgc cgctatcctt tcgaggagga gacagcgcta tccaatacgg accgatcccc
     5881 ctgaacctgc ccgaatccgg gctgagcgcg ctcttcggtg tccacgacga aaacctgagg
     5941 ctcctggaag acgccttcgg cgtcgagatc tcggcgcggg gcggcgtcgt ctccctgagc
     6001 gggggcccgg aaggcaccga gcgggccgcg cacctccttt ccgagttctc gaagctgatc
     6061 ggccggggct acgtgccgaa gaagtccgac gtgccgagcg cgatccggat cgtaaaggac
     6121 gacccgacgg cttcgctcgt cgactttttc gagaaccgtt ccgtcggcgc cgccctccgg
     6181 caggtcgtcg ctcctcgcaa cgtccgccag caggagtacc tgcaggcgat cggcgagcgg
     6241 gacgtcgtct tcgcgatcgg gcccgccggc accggaaaga cctacctcgc cgtcgcgatg
     6301 gccgccgcgg cgctcctcga gaagcgggtc aagcggatcg tcctctgccg gccggcggtc
     6361 gaggccggcg agaagctcgg cttcctcccg ggcgacctcg ccgagaaggt gaatccctac
     6421 cttcgcccgc tctacgacgc cctctacgac atcctcggct acgagaaggt cggacggctc
     6481 ctcgagagac aggtcatcga gatcgcaccg ctcgccttca tgcgcggccg gacgctgaac
     6541 gacgcgttca tcatcctcga cgaggcgcag aacacgacct ccgagcagat gaagatgttc
     6601 ctgacgcgca tcggcttcgg cgcgaaggcg gtcatcacgg gggacatc
//
LOCUS       DAOTEZ010000227         6639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_8251_length_6639_cov_6.782047, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000227 DAOTEZ010000000
VERSION     DAOTEZ010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6639
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8251_length_6639_cov_6.782047"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..5389)
                     /locus_tag="P5164_12385"
     CDS             complement(<1..5389)
                     /locus_tag="P5164_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRY44731.1"
                     /translation="MEDTSARAVAVVGVGAIMPGAPNARAFWENVTKGVYGISEVDPK
                     RWDPALYWDPDPKAPRKTYSKIGGWVREFPWEPVKWRLPIPPLVEAAMDDGQKWAIAI
                     TREVLADFGYPERPLDLDRTAVILGNAMGGEKHYQTAFHIFTPEVEAELLASPAFAAL
                     PPDVRARTIDEMRRGVAGRLPDVTEDTMPGELSNCIAGRVANLFNFHGANYVTDAACA
                     SALAAMSAAVDGLVAGKFDAVLTGGIDRNMGPTSFVKFCKIGALSATGSRPYADGADG
                     FVMGEGAAFFLLKRLADAERDGDRIYAVIRGIGASSDGRGKGITAPNPVGQRFALRHG
                     WQVAGLDPATCSYVEGHGTSTKVGDVVEVESLIEAFGAAGLPAHSIPLGSVKSNIGHL
                     KAGAGAAGFFKAAMALAHKVVPPSLNFERPNPNLDWSRSPFYVNTALREWAKPACGVR
                     RAGLSAFGFGGTNFHVVLEEHVPGALTKGSRPAQVSVSASPAASPAAAPKPPLRGALL
                     VGAADEKELAKKLHAVQDEARQGRAPAPAPPKGSDLRAPERVAIDFGSAHELAEKIEK
                     ALKALAGGKPAAWRPLRGQGIFRGHGPAPKVAFLFTGQGSQYVNMARTLRDNEPVVAE
                     TFAQADRRMEPILGRTISSYIFLDTKEPAAIAAAEEELKQTSITQPAVLTTDIALGRL
                     LNEYGIAPDMVMGHSLGEYGALVTAGVLPLEDALEAVAARGREMSRVSVADNGTLAAV
                     FAPVDVIEKTLEGIEGYVVVANYNSNGQSVIGGSTASVAAALEAFKAAGVTTAYIPVS
                     HAFHTKIVEPATVPFRTVLERLRIGGPKLPVVANVTGDLYPTDGDVRTKAIDLLSRQI
                     ASPVRFVKGLERLYEAGVRVFVEVGPKRAVQGFVEDVLGHRPDVHSLATNHPRLGDVV
                     SFNQAICGFWAAGIGTPLPEEKPRPVELAPARPVVPATLPAPAAAPPAPTSPVAPAAP
                     AAPLLASGGSGDPIRDLGLLFSEFLEKGMKIHRGEGVAIPAPAAPAEEPVVITGSSLG
                     LPGRPRMFQDDNVAALLRGDPLIGRIPERILRAMATKHITRLVKSEHGESRFEEVTST
                     DEVIKLAGRAGDLDLAEEFGVPAERIPALDVVTQLAIGAGLEALHDAGIPLVMRYKTT
                     TKGTKLPERWVLPEALQDDTGIVFASAFPGYDSFAERLRGYFLDHERRERLADLQALR
                     ATLAGKGASAEALAEMDGRIADLKALIDREHYTFDRRFLFQILSMGHSQFAELIGARG
                     PNTQVNAACSSTTQAVSVAEDWIRTGRARRVLVIAADDATSDQMMEWIGAGFVASGAA
                     ALDERVEDAALPFDRRRHGMIIGMGAVGLVVESAEAARERGITPACRVLSAVTANSAF
                     HGTRLDVNHISQVMETLVSSAERKWGIDRHAIAPRMVFVSHETYTPARGGSASAEIFA
                     LRGTFGSSVDSIVMANTKGFTGHPMAVGIEDALAVKMLETGIVPPIPNFREVDPELGP
                     LHLSAGGRYDRDYALRLAAGFGSQIAMTLLERVPPPDGRRRAPEELGYAYRIHDAGRY
                     RAWLSAISGYDAPETEVEKRTFRVKNTGAPIRASQRLDSPAGPASSRTAVPAPAPAPV
                     PAPTAPVVAPSAPAPAVAPAPAPVAAAAATDPVAERVLAIVAEKTGYAQEMLALDLDL
                     EADLGIDTVKQAELFSAVREEYGIPRDDSRKLRDYPTLAHVIQFVRDGRPDLPPPGAG
                     VPVPAAAPVPVPAAAVAVVAPAPAAPAVDPVLTRVLAIVTEKTGYPAEMLDPELDLEA
                     DLGVDTVKQAEL"
     gene            complement(5404..>6639)
                     /locus_tag="P5164_12390"
     CDS             complement(5404..>6639)
                     /locus_tag="P5164_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRY44732.1"
                     /translation="VVEDGKTVGYRINGAKQWISNGGYADVYTILANAPAGPTWFILE
                     KGAEGFSHGKPEDKHGLRASNTAALSLSDVYVPVENLVGGVEGQGLLQAQLVFGYTRL
                     MVAAFGLGAGWAALDRAIPYSKDRLQGGAPLSEKQGYTHKLIVPHAVRLEAGRAYIEE
                     TAERLDSGEEMLNTEGAIAKYLATEAGLAAAEASIQALGGYGYTREYMVEKIRRDVRI
                     TTIYEGTSEIMEMTIARDRWQQHLKTRGQHYHDKAREMEALEAAQPGCGARFAALALH
                     SLAEGLERFRAQRLTRHQHVLFRAGRMIALAEGAMVLAKRAARAAKGELPEKADRRLD
                     AAALAAVSRANGREAAFEVAQEALRWAVGADGVPASDLAAFTAAIRLPEISGAQAGLM
                     ADLDLVSDALYGRTPAPRA"
BASE COUNT          855 a         2249 c         2599 g          936 t
ORIGIN      
        1 agagctcggc ctgcttgacc gtgtcgacgc cgaggtccgc ctcgaggtcg agctcggggt
       61 cgagcatctc ggccgggtag ccggtcttct cggtcacgat cgcgaggacg cgcgtcagca
      121 ccgggtcgac ggcgggagcg gccggtgcgg gagcgacgac cgcgaccgcg gcggccggaa
      181 ccggcacagg cgccgcggcg ggcaccggaa ccccggcgcc gggcggcggc aggtcgggcc
      241 ggccgtcgcg gacgaactgg atcacgtgcg cgagcgtcgg gtagtcgcgc agcttgcggc
      301 tgtcgtcgcg cgggatgccg tactcctcgc gcacggcgct gaagagctcg gcctgcttga
      361 cggtgtcgat cccgagatcg gcctccaggt cgaggtcgag cgcgagcatc tcctgcgcgt
      421 agccggtctt ctcggccacg atcgcgagga cgcgctcggc gacaggatcg gtcgcggccg
      481 cggcggcgac cggagcgggt gcgggtgcca cggccggagc cggggcggac ggggccacga
      541 ccggggcggt gggtgccggg acaggcgccg gggcgggcgc gggaaccgcc gtccgcgagg
      601 acgccggccc ggccggcgaa tcgagccgct gcgaggcgcg gatcggggct ccggtgttct
      661 tcacgcggaa cgtccgcttc tcgacctcgg tctcgggcgc gtcgtagccg ctgatcgcgg
      721 agagccaggc gcggtagcgc ccggcgtcgt ggattcggta ggcgtagccg agctcttccg
      781 gagcgcgccg gcggccgtcg ggcggcggga cgcgctcgag gagcgtcatc gcgatctgcg
      841 agccgaagcc ggcggcgagg cggagcgcgt aatcgcggtc gtagcgcccg cccgcagaca
      901 ggtggagcgg tccgagctcg gggtcgacct cccggaagtt cgggatcggc gggacgatcc
      961 ccgtctcgag catcttgacc gcgagggcgt cctcgatgcc gaccgccatc gggtggcccg
     1021 tgaagccctt cgtgttcgcc atgacgatgg agtcgacgga ggagccgaac gtcccccgga
     1081 gggcgaagat ctcggccgaa gcgctgccgc cgcgggcggg cgtgtaggtc tcgtgagaga
     1141 cgaagaccat cctcggggcg atcgcgtggc ggtcgattcc ccacttccgc tcggccgagg
     1201 agacgagcgt ctccatgacc tgcgagatgt ggttcacgtc gagccgcgtg ccgtggaagg
     1261 cgctgttcgc cgtgacggcc gagaggacgc ggcaggccgg ggtgatcccg cgctcgcgag
     1321 ccgcctcggc gctctcgacg accaggccga cggcccccat tccgatgatc attccgtggc
     1381 ggcggcggtc gaacgggagg gcggcgtcct cgactctctc gtcgagcgcc gccgcgccgg
     1441 aggccacgaa gccggcgccg atccactcca tcatctggtc ggaggtcgcg tcgtcggcgg
     1501 cgatgacgag gacgcggcgg gcgcggcccg tgcggatcca gtcctcggcg accgagaccg
     1561 cctgcgtcgt cgaggagcag gcggcgttga cctgggtgtt cgggccgcgc gcgccgatca
     1621 gctcggcgaa ctgcgagtgg cccatcgaga ggatctggaa gaggaagcgc cggtcgaagg
     1681 tgtagtgctc ccggtcgatg agcgccttca ggtcggcgat ccgcccgtcc atctcggcga
     1741 gggcctcggc cgacgcgccc ttgccggcga gcgtcgcgcg gagcgcctgg agatcggcga
     1801 gccgctcgcg ccgctcgtgg tcgaggaagt agccgcggag ccgctcggcg aacgagtcgt
     1861 agcccgggaa ggccgacgcg aagacgatgc ccgtgtcgtc ctggagcgcc tcggggagga
     1921 cccagcgctc ggggagcttc gtccccttcg tcgtcgtctt gtagcgcatc acgagcggga
     1981 tccccgcgtc gtggagcgcc tcgaggcccg cgccgatcgc gagctgcgtg acgacgtcga
     2041 gagccgggat gcgctcggcc ggaacgccga actcctcggc gaggtcgagg tcgccggctc
     2101 ggccggcgag cttgatcacc tcgtcggtcg acgtgacctc ctcgaagcgg ctctcgccgt
     2161 gctcgctctt gacgagccgg gtgatgtgct tcgtcgccat cgcccggaga atcctctcgg
     2221 ggatccggcc gatgagcggg tcgccgcgga ggagagcggc gacgttgtcg tcctggaaca
     2281 tccgcggccg gcccggcagg ccgagcgacg acccggtgat gacgaccggc tcctcggcgg
     2341 gcgccgccgg cgcagggatc gcgacgccct cgccgcggtg gatcttcatc cccttctcga
     2401 ggaactcgga gaagaggagg ccgaggtcgc ggatcgggtc gcccgagccg ccggaagcca
     2461 ggagaggcgc cgcgggcgcg gcgggtgcaa cgggtgaggt cggcgccggg ggtgcggccg
     2521 ccggagcggg gagcgtcgcc gggacgaccg gccgcgcggg ggcgagctcg acggggcgag
     2581 gcttctcctc cgggagcgga gtgccgatgc cggcagccca gaagccgcag atcgcctggt
     2641 tgaaggagac gacgtcgccg aggcgcgggt ggttcgtggc gagggagtgg acgtcggggc
     2701 ggtggccgag gacgtcctcg acgaagccct ggacggcccg cttcgggccg acctcgacga
     2761 agacgcggac acccgcctcg tagaggcgct cgagcccctt cacgaagcgg acgggcgagg
     2821 cgatctggcg ggagaggagg tcgattgcct tcgtccgcac gtcgccgtcc gtcgggtaga
     2881 ggtcgcccgt gacgttcgcg acgaccggga gcttcggccc cccgatccgg agccgctcga
     2941 ggaccgtccg gaaggggacg gtcgccggct cgacgatctt cgtgtggaac gcgtggctca
     3001 cggggatgta cgccgtcgtg acgccggccg ccttgaacgc ctcgagggcg gcggcgaccg
     3061 aggcggtcga gccgccaatg acggactggc cgttgctgtt gtagttcgcg acgacgacgt
     3121 acccctcgat tccctcgagc gtcttctcga tgacgtcgac gggggcgaag acggcggcga
     3181 gggtcccgtt gtcggcgacg gagacgcgcg acatctcgcg gccgcgggcg gcgacggcct
     3241 cgagcgcgtc ctcgagcggg aggacgcccg ccgtgacgag ggcgccgtac tcgccgaggg
     3301 agtgccccat gaccatgtcg ggggcgatgc cgtactcgtt gaggagccgg ccgagggcga
     3361 tgtcggtcgt caggaccgcg ggctgcgtga tggaggtctg cttcagctcc tcttcggccg
     3421 ccgcgatcgc cgcgggctcc ttggtgtcga ggaagatgta ggagctgatc gtgcggccga
     3481 ggatcggctc catcctccgg tcggcctgcg cgaaggtctc cgcgacgacg ggctcgttgt
     3541 cgcgcagcgt ccgcgccatg ttcacgtact gcgagccctg gccggtgaag aggaaggcga
     3601 ccttcggcgc cgggccgtgg ccgcggaaga tgccctggcc gcggagcggc cgccaggcgg
     3661 cgggcttgcc gcccgcgagc gccttgagcg ccttctcgat cttctcggcg agctcgtgcg
     3721 cgctgccgaa gtcgatcgcg acgcgctcgg gggcgcggag gtcgcttccc ttcggcgggg
     3781 ccggagcggg cgccctcccc tgccgcgcct cgtcctggac ggcgtggagc ttcttcgcga
     3841 gctccttctc gtcggccgcg ccgacgagga gcgcgccgcg gagcggcggc ttcggcgcgg
     3901 cggcgggcga agcggccggc gacgcggaga ccgagacctg cgcgggacgg cttcccttcg
     3961 tgagcgcgcc ggggacgtgc tcctcgagga cgacgtggaa gttcgttccg ccgaagccga
     4021 aggccgagag gcccgcgcgg cggacgccgc aggcgggctt cgcccactcg cgcagcgcgg
     4081 tgttcacgta gaacggcgac ctcgaccagt cgaggttcgg gttcggccgc tcgaagttga
     4141 ggctgggcgg gacgaccttg tgcgcgagcg ccatcgccgc cttgaagaag ccggcggcgc
     4201 ccgcgcccgc cttgaggtgc ccgatgttcg acttgaccga accgagcggg atggagtgcg
     4261 ccgggaggcc cgccgcgccg aacgcctcga tgaggctctc gacctcgacg acgtcgccga
     4321 ccttcgtcga cgtgccgtgc ccctcgacgt aggagcaggt ggccgggtcg agccccgcga
     4381 cctgccagcc gtggcggagc gcgaagcgct ggccgaccgg gttcggggcg gtgatcccct
     4441 tgccgcgccc gtcgctcgag gcgccgatgc cgcggatgac ggcgtagatc cggtcgccgt
     4501 cgcgctcggc gtcggcgagg cgcttcagga ggaagaaggc cgccccctcg cccatgacga
     4561 agccgtcggc cccgtcggcg taggggcgcg agccggtggc cgagagcgcg ccgatcttgc
     4621 agaacttgac gaagctcgtc ggccccatgt tccggtcgat tccgcccgtg aggacggcgt
     4681 cgaacttccc ggccacgagg ccgtcgaccg ccgcgctcat cgccgcgagg gcggaggcgc
     4741 aggcggcgtc ggtgacgtag ttcgccccgt ggaagttgaa gaggttcgcg acacggccgg
     4801 cgatgcagtt gctgagctcg cccggcatcg tgtcctcggt gacgtcgggg aggcggccgg
     4861 cgacgccccg gcgcatctcg tcgatcgtcc gggcgcggac gtcgggcggg agggccgcga
     4921 acgccggcga ggcgagcagc tcggcctcga cctccggcgt gaagatgtgg aaggcggtct
     4981 ggtagtgctt ctcgcccccc atcgcgttcc cgaggatgac ggccgtccgg tcgaggtcga
     5041 gggggcgctc ggggtagccg aagtcggcga ggacctcgcg cgtgatcgcg atcgcccact
     5101 tctgcccgtc gtccatcgcc gcctcgacga ggggcgggat cgggagcctc cacttcaccg
     5161 gctcccaggg gaactcgcgg acccagccgc cgatcttcga gtaggtcttc cgcggcgcct
     5221 tcgggtcggg gtcccagtag agggccgggt cccaccgctt cgggtcgacc tcggagatcc
     5281 cgtagacccc cttcgtgacg ttctcccaga aggcgcgcgc gttcggcgcg ccgggcatga
     5341 tcgcaccgac gccgacgacg gcgaccgcgc gggcagacgt gtcttccatg gggtggtgtc
     5401 ctctcaggcc cgcggagcgg gcgtccgccc gtagagggcg tcggagacga ggtcgaggtc
     5461 ggccatcagg ccggcctgcg ccccggaaat ctcggggagg cggatcgccg cggtgaacgc
     5521 ggcgaggtcg gaggcgggga cgccgtcggc gccgaccgcc cagcggagcg cctcctgcgc
     5581 cacctcgaag gcggcctcgc ggccgttcgc gcgggagacg gcggcgaggg cggctgcgtc
     5641 gaggcgccgg tcggccttct cggggagctc ccccttcgcg gcgcgggcgg cccgcttcgc
     5701 gaggaccatc gccccctcgg cgagggcgat catccggccc gcgcggaaga ggacgtgctg
     5761 gtggcgcgtc aggcgctgcg cgcggaagcg ctcgagcccc tcggcgaggg agtggagcgc
     5821 gagggcggcg aagcgggcgc cgcagccggg ctgcgcggcc tcgagcgcct ccatctcgcg
     5881 ggccttgtcg tggtagtgct ggccacgcgt cttcaggtgc tgctgccagc ggtcgcgcgc
     5941 gatcgtcatc tccatgatct ccgaggtccc ctcgtagatc gtcgtgatgc ggacgtcgcg
     6001 ccggatcttc tcgaccatgt actcgcgggt gtagccgtag ccgccgagcg cctggatcga
     6061 ggcctccgcc gcggcgaggc ccgcctcggt cgcgaggtac ttcgcgatgg ccccctcggt
     6121 gttcagcatc tcctcgcccg agtcgagccg ctcggccgtc tcctcgatgt aggcgcggcc
     6181 ggcctcgagg cgcacggcgt gcgggacgat gagcttgtgg gtgtagccct gcttctcgga
     6241 gaggggcgcc ccgccctgga ggcggtcctt cgagtacggg atcgcccggt cgagcgcggc
     6301 ccagccggcg ccgaggccga acgcggcgac catgaggcgc gtgtagccga agacgagctg
     6361 ggcctggagg agcccctgcc cctcgacgcc gccgacgagg ttctcgaccg gaacgtagac
     6421 gtcggacagc gaaagcgccg ccgtgttcga ggcgcgcagg ccgtgcttgt cctcgggctt
     6481 gccgtgcgag aagccctcgg cccccttctc gaggatgaac caggtggggc cggcgggagc
     6541 gttcgcgagg atcgtgtaga cgtcggcgta gccgccgttg ctgatccact gcttggcgcc
     6601 gttgatccgg tacccgacgg tcttcccgtc ctcgacgac
//
LOCUS       DAOTEZ010000228         6623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_8278_length_6623_cov_6.044153, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000228 DAOTEZ010000000
VERSION     DAOTEZ010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6623
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8278_length_6623_cov_6.044153"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>926)
                     /locus_tag="P5164_12395"
     CDS             complement(<1..>926)
                     /locus_tag="P5164_12395"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HRY44733.1"
                     /translation="RLLRVWPSHRVPLVSCLGFTDIRHGPYALSVRPCRSFRLEAHEQ
                     GLLLVFDGEAGEAARREGGTVLYKAYTETKRWNELVGVGTVPDLNDLCLGDRIKHVIR
                     ITEGQHEKKIAELADRIASRSHELRVVCIAGPSSSGKTTFVKRLSIQLEVNGITPRTL
                     SLDDYYVDREMTPRDAEGDLDFEALEAIDLPLFLLHLKELVDGREVLTPIYDFRTGMR
                     KEKAEWRPRRLGRNEVLLIEGIHALNPRLTETVDERHKFRIFINALTQLCIDDHNRIR
                     TSDARLLRRIVRDRRYRNYSAETSILRWPKVRA"
     assembly_gap    927..936
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<937..1424)
                     /locus_tag="P5164_12400"
     CDS             complement(<937..1424)
                     /locus_tag="P5164_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44734.1"
                     /translation="MSAGSGLALIPVELPWTKTEVVEGTTVDELFDAGPTEALDVVAA
                     CVNSRVVPLDFAIECPSRVEPVDSASKEGEEVVRRTATLLLHGVARKLFPKARLHVGQ
                     SLRGGYHYGVSGEHPPLAELAATLDEAFHREADAGRQVVRQEVSIDSALHLFEQEGEP
                     LKV"
     gene            complement(1421..3193)
                     /locus_tag="P5164_12405"
     CDS             complement(1421..3193)
                     /locus_tag="P5164_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44735.1"
                     /translation="MIDARARRDRIAQWAFDARPVLGRFHLWLEDVEESRVEGEAGLD
                     LSFAGTSLEKCLIMTAGVTALGTRLFGRYGEAKGLDAHGANHVKKDADAVSAYAMSEG
                     LWYLTRSLPENHAIMVSLGEGLMPKGGETPDMGSNPLLGFGRVYARPSVAKWLDARVH
                     RFINDASYGWRAFWSELMAARITVWGAAIDTLENTSRFAKGAPTGPTTVLHLFDQPVR
                     IARPYEGYTGAMTLPKEVYETAADSSILLDFLTPRALVLDAIRKTYPGIEPGDVHVWT
                     LAGPTRVKRIGRLWDEWKSLGAHLVEDGWTLPSGLGAFTDSGTYAPTYRVGPFTGPDG
                     RTHLFVTDGYAASAEALQAASLDPILGNRTSMCVLTSKFEVSYERERQILFLDPAAPD
                     FPQRLAGILEREVTPKDCLAYAGILTNARDAAMPLGKRTVTADDFFQKKEWHVLSLMG
                     SMLPEPYTGLAGVEQVKQGVYRVTTRAASERGVLDVTLTLRLMESFEQTRLVFSPLLD
                     RFYRGQDWKTRPVKVSDSGRIRNELQTLASEALEYFGENGIRVHFDRVDDAVLAPDKK
                     VLIREVLAWYKEHHPIWFGWLELA"
     gene            complement(3226..>4769)
                     /locus_tag="P5164_12410"
     CDS             complement(3226..>4769)
                     /locus_tag="P5164_12410"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HRY44736.1"
                     /translation="LELCPHAEELFDRIVWENGAVAWGARGARPLAPPVPGELEEALA
                     RRGVALRRGQAILAGHARDAGAALEEIGRLGLEVQVVRNRQELMLVPSGVSKGTGLFD
                     ALGDLGISRHSTVAIGDAENDHSLLGACELGVAVADAVPSLRAAADVVLPEAGSAALA
                     RFLLGPAVLSDETPEPRRFQAVLGTYEDGSLARLPASGVNVLVTGGSMSGKSHLTGLL
                     AERLLALGYSLCVLDPEGDHVALGRLRGVLAVGGNEPLPGPDQLPRLIEHRFGSLVVD
                     LSFLDPSEKTAYARAALVELSALWNDTGLPHWLFLDEAQVALNLSQLPVEGFDPGRPG
                     TCLSTWRPEDLPADVVGSLDVVLDLGAEETAGLPEWARSAGARPPPGQALLSRRTSPG
                     PRRFTVSRRASPHARHLHKYIGGSLPPEQRFFFRSGAGATGRAAGNVEEFHRELRRAE
                     ADVVRHHALHGDLSRWVADVLRDEVLAATVRTAERALRHRASAYEIESLRTEVLAAIL
                     DRYQG"
     assembly_gap    4770..4779
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5162..6229
                     /locus_tag="P5164_12415"
     CDS             5162..6229
                     /locus_tag="P5164_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRY44737.1"
                     /translation="MTIAFLDAGFYAHPDLVQPRQRILRYVDVTDPRAAYPRLLRPDE
                     SSWHGMMTSVVACGNGTLSDGLYRGLASESNVVLVKVGTARRITHENLRRGLEWVVRN
                     RARYGIRIANVSCGGDYEASYLHDDLSQAAEKATRSGILVVAAVGNLGLTPGHPVLPP
                     ASAPSVLTVGGLDDGNHLHRAGHDIYHGSWGPTVDGLQKPEVIAPAIWVAAPILPGTP
                     TSAEASLHVHLATSRDDELDVILEAHAGVDPALDAARHLAPPLLRQLVEAKLRDGNVI
                     SGAYKHVDGTSFAAPIVSSLAAQMLEANPDLRPHEVKSILVSTARRLPHVPVDQQGWG
                     VVEPADAVEEALARAGAGPAA"
     gene            complement(6231..>6623)
                     /locus_tag="P5164_12420"
     CDS             complement(6231..>6623)
                     /locus_tag="P5164_12420"
                     /inference="COORDINATES: protein motif:HMM:NF027675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HRY44738.1"
                     /translation="HLVELFYEGWSVKADATAGTATIERPRGRFALREMNFLHLNEPK
                     GLWTWAADAYAVVLGFLAVTGLFVLKGKNGLAGRGKWLAGLGVLLPILALFLLLWRPG
                     DSGPRGEGEGNGGGRHRGDGRAEAGPER"
BASE COUNT          917 a         2375 c         2400 g          911 t
ORIGIN      
        1 gcgcggacct tcggccagcg caggatcgac gtctcggccg agtagttccg gtaccggcgg
       61 tcccgcacga tccggcggag cagcctcgcg tcgctcgtgc ggatccggtt gtggtcgtcg
      121 atgcagagct gcgtcagggc gttgatgaag atgcggaact tgtgcctctc gtccacggtc
      181 tcggtgaggc gcgggttcag cgcgtggatc ccctcgatga ggaggacctc gttccgcccg
      241 agccggcgag ggcgccactc ggctttctcc ttccgcatcc cggtccggaa gtcgtagatc
      301 ggcgtcagga cctccctccc gtcgacgagc tccttcaggt ggaggaggaa gagcgggagg
      361 tcgatcgcct cgagcgcctc gaagtcgagg tcgccctccg cgtcgcgcgg ggtcatctcc
      421 cggtcgacgt agtagtcgtc gagggagagc gtccgcgggg tgatgccgtt cacctcgagc
      481 tggatggaga ggcgcttgac gaacgtcgtc ttccccgacg aggacgggcc cgcgatgcag
      541 acgacccgga gctcgtggct ccgcgacgcg atccggtcgg cgagctcggc gatcttcttc
      601 tcgtgctgcc cttccgtgat ccggatgacg tgcttgatcc ggtcgccgag gcagaggtcg
      661 ttgaggtcgg ggaccgtccc gacgccgacg agctcgttcc agcgcttcgt ctccgtgtag
      721 gccttgtaga ggaccgtccc gccctcccgt cgggccgctt cgcccgcctc gccgtcgaag
      781 acgaggagga gcccctgctc gtgcgcctcc aagcggaagc tccggcaggg tcggacggag
      841 agcgcgtagg ggccgtgccg gatgtcggtg aagccgaggc aggagacgag cggcacccgg
      901 tgggacggcc agacccggag gagacgnnnn nnnnnnacct tgagcggctc gccctcctgc
      961 tcgaagaggt ggagcgcgga gtcgatggag acctcctgcc tcacgacctg gcgaccggcg
     1021 tcggcctccc ggtggaacgc ctcgtcgagc gtggcggcga gctcggcgag cggcgggtgc
     1081 tcgccggaga cgccgtagtg gtagccgccc cggaggctct ggccgacgtg gagacgcgcc
     1141 ttcgggaaga gctttcgcgc gacgccgtgg aggaggagcg tcgccgtcct gcggacgacc
     1201 tcctcccctt ccttcgacgc cgagtcgacg ggctcgaccc ggctcgggca ctcgatcgcg
     1261 aagtcgagcg ggacgacgcg gctgttgacg cacgccgcga cgacgtcgag ggcctcggtc
     1321 ggcccggcgt cgaacagctc gtcgaccgtc gtcccctcga cgacctccgt cttcgtccac
     1381 gggagctcga cggggatcag ggcgaggccg gaacccgcgc tcatgcgagc tcgagccagc
     1441 cgaaccagat cgggtggtgc tccttgtacc aggcgaggac ctcccggatg aggaccttct
     1501 tgtcgggcgc gaggacggcg tcgtcgaccc ggtcgaagtg gacgcggatg ccgttctcgc
     1561 cgaagtactc gagcgcttcc gacgcgagcg tctggagctc gttgcggatc cggcccgagt
     1621 cggagacctt caccggccgc gtcttccagt cctggccgcg gtagaagcgg tcgaggagcg
     1681 gcgagaagac gaggcgcgtc tgctcgaacg actccatcag gcggagcgtc agcgtcacgt
     1741 ccaggacccc gcgctccgag gccgcacgcg tcgtgacgcg gtagaccccc tgcttcacct
     1801 gctcgacgcc cgcgaggccg gtgtagggct cggggagcat gctccccatg agcgagagga
     1861 cgtgccactc cttcttctgg aagaagtcgt cggcggtgac ggtccgcttg ccgagcggca
     1921 tcgccgcgtc gcgggcgttc gtcaggatgc ccgcgtaggc gaggcagtcc ttcggcgtca
     1981 cctcgcgctc gaggatcccg gcgaggcgct gcgggaaatc cggggcggcg gggtcgagga
     2041 agaggatctg ccgctccctc tcgtaggaga cctcgaactt cgacgtcagg acgcacatcg
     2101 aggtccggtt cccgaggatc gggtcgaggc tcgccgcctg gagcgcctcg gccgaggccg
     2161 cgtagccgtc cgtcacgaag aggtgcgtcc gcccgtcggg tcccgtgaac ggcccgacgc
     2221 ggtaggtcgg ggcataggtc cccgagtcgg tgaaggcgcc gaggccgctc gggagcgtcc
     2281 agccgtcctc gacgaggtgg gcgccgagcg atttccactc gtcccagagc ctgccgatcc
     2341 gcttcacgcg cgtcggcccc gcgagcgtcc agacgtggac gtcgcccggc tcgatcccgg
     2401 ggtaggtctt ccggatggcg tcgaggacga gcgcccgcgg ggtcaggaag tcgagcagga
     2461 tcgaggagtc ggccgccgtc tcgtagacct ccttcgggag ggtcatcgcg ccggtgtacc
     2521 cctcgtaggg gcgcgcgatc cggaccggct ggtcgaagag gtggaggacc gtcgtcggcc
     2581 ccgtcggggc ccccttcgcg aagcgggacg tgttctcgag cgtgtcgatg gccgcgcccc
     2641 agacggtgat gcgcgccgcc atcagctcgc tccagaacgc ccgccagccg taggaggcgt
     2701 cgttgatgaa gcggtggacc cgcgcgtcga gccacttcgc gaccgagggg cgggcgtaga
     2761 cgcggccgaa cccgaggagc gggttcgagc ccatgtcggg cgtctcgcct cccttgggca
     2821 tcagcccctc gccgaggctc accatgatcg cgtggttctc cgggagcgac cgcgtcaggt
     2881 accagagccc ctcgctcatc gcgtaggccg acacggcgtc ggcgtccttc ttgacgtggt
     2941 tcgccccgtg ggcgtccagc cccttcgcct cgccgtagcg gccgaaaagg cgcgtgccga
     3001 gcgccgtgac gccggccgtc atgatgaggc acttctcgag cgacgtcccc gcgaacgaga
     3061 ggtccaggcc ggcctcccct tcgacgcggc tctcctcgac gtcctcgagc cagaggtgaa
     3121 agcggccgag cacgggacgg gcgtcgaagg cccactgagc gatccggtca cggcgtgcgc
     3181 gtgcgtcgat catgaagtcg agtttagcct cgcgcgcgac gtttcctagc cctggtagcg
     3241 gtcgaggatc gcggcgagga cctcggtccg gagcgattcg atctcatagg cggaggcacg
     3301 gtggcgaagc gcacgctccg ccgtccgcac cgtcgcggcg aggacctcgt cgcgcaggac
     3361 gtcggcgacc catcgcgaga ggtccccgtg gagcgcgtgg tgccggacga cgtcggcctc
     3421 ggcacgccgg agctcccggt ggaactcctc gacgttcccg gcggcgcgcc ccgtcgcgcc
     3481 cgcgccggag cggaagaaga accgctgctc gggagggagc gagccgccga tgtacttgtg
     3541 gaggtggcgc gcgtgcgggc tggcccgacg cgagaccgtg aaacggcgcg gccccggact
     3601 ggttctgcgg gacaggaggg cctgccccgg cggcggccgc gccccggccg aacgtgccca
     3661 ttccggcagg cccgcggtct cctcggcgcc gaggtcgagg acgacgtcga ggcttccgac
     3721 gacgtccgcg gggaggtcct cgggcctcca cgtcgacagg caggtccccg gacggccggg
     3781 gtcgaagccc tccaccggga gctgggagag gttcagagcg acctgcgcct cgtcgaggaa
     3841 gagccagtgc ggcaggccgg tgtcgttcca gagagccgag agctccacga gggcggcgcg
     3901 cgcgtaggcg gtcttctcgg acggatcgag gaacgagagg tcgacgacga ggctcccgaa
     3961 ccggtgctcg atcagccgcg gcagctggtc cgggccgggg agcggctcgt tccctccgac
     4021 ggcgaggacc ccgcggagcc ggccgagcgc gacgtggtcc ccctccgggt cgaggacgca
     4081 gagcgagtag ccgagcgcca ggagccgctc ggcgaggagg cccgtcaggt gcgacttccc
     4141 cgacatcgag ccgcccgtga cgaggacgtt caccccggag gccgggagcc gtgcgaggga
     4201 gccgtcctcg tacgtcccga ggacggcctg gaaccggcgc ggctccgggg tctcgtcgga
     4261 aaggacggcc gggccgagga ggaaccgggc gagcgccgcg ctgccggcct cggggaggac
     4321 gacgtcggcc gccgcccgga gggacgggac ggcgtcggcg acggcgacgc cgagctcgca
     4381 tgcgccgagg agggagtggt cgttctccgc gtccccgatc gccaccgtcg agtggcgcga
     4441 gatgccgagg tcgccgagcg cgtcgaagag cccggtcccc ttcgagacgc ccgacgggac
     4501 gagcatcagc tcctgccggt tcctcacgac ctgcacctcg agcccgagcc gcccgatctc
     4561 ctcgagcgcg gccccggcat cgcgggcgtg ccccgcgagg atcgcctgcc cccggcggag
     4621 cgcgacgccg cgccgtgcga gcgcctcctc cagctctccg ggaacgggcg gggccagcgg
     4681 gcgcgcgccc cgcgcgcccc acgcgaccgc cccgttctcc cagacgatcc ggtcgaagag
     4741 ctcctcggcg tgcgggcaga gctcgaggan nnnnnnnnng tgacgaggac cagcttcatc
     4801 cgctcctgcc gaagggtgcg gagcgcggcg aggaccgtct cgtcgagacg cccggtcgac
     4861 gtcagcgtgc cgtcgtagtc gagcgcgagc gcgcggaagt gctgcggcat cttccctccc
     4921 gcgagggtga tcgggccgga acgacgacgg gctctcggcg ggaactctat cacccgcgat
     4981 ggacctcggg cccgtcccgc gcgacgcgac ccctcccatt tctccggcaa cctgctagcg
     5041 tctccggagc gtgccgcctc ccgatcgccc gcccttcggc gtcccggcgc cccgagaccc
     5101 ggggccgatc ctcccgacgc ccgtgcggct cggcgcggac gagaggttcc gcggccgggg
     5161 cgtgacgatc gctttcctcg acgccgggtt ctacgcgcac cccgacctcg tccagccccg
     5221 gcaacggatc ctccgctacg tcgacgtcac cgacccgcgc gccgcctatc cgcggctcct
     5281 ccgccccgac gagtcgagct ggcacgggat gatgacctcc gtcgtcgcct gcgggaacgg
     5341 caccctctcc gacggcctct accgcggcct cgcctcggag tcgaacgtcg tcctcgtgaa
     5401 ggtgggaacg gcccggcgca tcacgcacga gaacctccgc cgcggcctcg agtgggtcgt
     5461 gcggaaccgc gcgcgctacg gcatccggat cgcgaacgtc tcctgcgggg gggactacga
     5521 agcgtcgtac ctccacgacg acctctcgca ggccgccgag aaggcgaccc gctcgggaat
     5581 cctcgtcgtc gcggccgtcg ggaacctggg gctcacgccg ggccaccccg tcctcccgcc
     5641 cgcctcggcg ccgtccgtcc tgacggtggg cggcctcgac gacggcaacc acctccatcg
     5701 cgccgggcac gacatctacc acggcagctg gggcccgacc gtcgacgggc tccagaagcc
     5761 cgaggtgatc gcccccgcga tctgggtggc ggcgccgatc ctccccggca ccccgacgtc
     5821 cgccgaagcg tcgctccacg tccacctcgc cacgtcgcgc gacgacgagc tggacgtcat
     5881 cctcgaagcg cacgcgggcg tcgacccggc gctcgacgcg gcccgacacc tcgcgccgcc
     5941 gctccttcgg cagctcgtgg aggcgaagct gcgcgacggc aacgtcatca gcggggccta
     6001 caagcacgtc gacgggacga gcttcgcggc gccgatcgtc tcctcgctgg cggcccagat
     6061 gctcgaggcg aaccccgacc tccgcccgca cgaggtcaag tcgatcctgg tgtcgacggc
     6121 gcggcggctg ccgcacgtcc ccgtcgacca gcagggctgg ggcgtcgtgg agccggccga
     6181 cgccgtggaa gaggcgctcg cgcgggccgg cgccgggccg gctgcgtagc tcagcgctcc
     6241 ggcccggcct ccgcgcgccc gtccccgcgg tggcgccccc ctccgttccc ctcgccctcc
     6301 ccgcgcggcc cggagtcgcc cgggcgccag aggaggagga agagcgcgag gatcggcagg
     6361 aggacgccga ggccggccag ccacttgccc cgccccgcga ggccgttctt ccccttcagg
     6421 acgaagaggc ccgtgacggc gaggaagccg agcacgaccg cgtaggcgtc ggccgcccag
     6481 gtccagagcc ccttcggctc gttcaggtgg aggaagttca tctcgcggag cgcgaagcgg
     6541 ccgcgcggac gctcgatcgt cgccgtcccc gccgtcgcgt cggccttcac gctccacccc
     6601 tcgtagaaga gctcgacgag gtg
//
LOCUS       DAOTEZ010000229         6548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_8409_length_6548_cov_6.361928, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000229 DAOTEZ010000000
VERSION     DAOTEZ010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6548
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8409_length_6548_cov_6.361928"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /gene="groEL"
                     /locus_tag="P5164_12425"
     CDS             complement(<1..842)
                     /gene="groEL"
                     /locus_tag="P5164_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRY44739.1"
                     /translation="MAAKNITYGEDARQNILRGVNKLADAVKVTLGPKGRNVVIEKKF
                     GSPLITKDGVTVAKEIELPEPLENMGAQMVREVASKTSDIAGDGTTTATVLAQAIYRE
                     GIKMVTSGHNPMELKRGIEIAVKKSVESIDKLKKSVDAKEIAFVGTISANGDEEIGKI
                     IAEAMDKVGKDGVITVEEAKGLETTLEVVEGMQFDRGYLSPYFVTDAERMEAVLENAR
                     ILIFEKKISSMKDLLPVLEQTAKQGKQLLIIAEDIEGEALATLVVNKLRGTLQVCAVK
                     APGFG"
     gene            complement(858..1151)
                     /locus_tag="P5164_12430"
     CDS             complement(858..1151)
                     /locus_tag="P5164_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRY44740.1"
                     /translation="MKIRPLYDRILVKRIEPKEQVRGGIIIPDTAKEKPMEATVEAVG
                     EGKFDDNGKRVALTVKAGDRILIGKYAGTEIKIDDNEYLILREDEVLAIVDAK"
     gene            complement(1323..2468)
                     /locus_tag="P5164_12435"
     CDS             complement(1323..2468)
                     /locus_tag="P5164_12435"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRY44741.1"
                     /translation="MPLAVPVPLLDLKKQYATVRDEIRAATDELFESQGFILGPKVEA
                     FEKAIAEYVGVKHAIGMSSGTDAQLAAMMALGIGPGDDVVTSPYTFFASAGAVVRLGA
                     RPVFCDIDPATYNVDPEKLEKAITTRTKAIQPIHLYGQCADMDPILDVAKAKGIPVIE
                     DACQSLGAGYKGKKAGALGDFGAFSFFPSKNLGGFGDGGMVTTNDDAMAATLRALRMH
                     GETSRYHHKLVGGNFRLDALQAVVLHVKLPHLDAWAEGRRQNAADIERRFLEHGGRTF
                     EEGGIAFPREAPGRHHVYNQFVVRVARGRRDELKDRLTQLKVGCAIYYPVPLHLQECF
                     ANLGGKAGDFPVAEQAALETLALPVFPELSPSQKAFLAYHLAQFSKE"
     gene            complement(2538..3248)
                     /locus_tag="P5164_12440"
     CDS             complement(2538..3248)
                     /locus_tag="P5164_12440"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRY44742.1"
                     /translation="MIPLRDTIPSRTVPIVRSAILAACVGVFVLQLLAGNGGQALVDA
                     FGFVPARLFHSGELGPGSFSLGLAGLFASMFLHGGLMHLAGNMLFLWIFGDNVEDALG
                     HGRFLLLYVGSGVLAALLQGVLAPSSLVPMVGASGAIAGVLGAYFVLYPHARVVTLVP
                     LFFLFPLVEVPAFLFLLLWFLLQFWQGSAALVASGKAGAAAGGVAWWAHVGGFAAGIA
                     LLALLKPRRRPAPRYWIR"
     gene            complement(3397..3684)
                     /locus_tag="P5164_12445"
     CDS             complement(3397..3684)
                     /locus_tag="P5164_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRY44743.1"
                     /translation="MAGKAEIVEAISKETELSKKDATSAFEAFVNSIQDSLKKGERVA
                     VPGLGIFSIAERKARVGINPQTKAKIKIAASKVAKFKAGKDLKTLINKKRK"
     gene            complement(3813..5159)
                     /locus_tag="P5164_12450"
     CDS             complement(3813..5159)
                     /locus_tag="P5164_12450"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRY44744.1"
                     /translation="MTDGVPVRALAVEVLVRIERDRAFAAPLLAAKEGRLPPRDRGLL
                     RTIVLSALRNRALLDHVLTRHLSRPIETLEADVRAGLRVGAAQLLLLQRVPPHAAVGE
                     TVGAVRLRVPRAAGLVNAVLRKVAGEGKPPRIDLPPGTDPLVRLALETSHPEWLVRRW
                     AADLGLEATEAALLSDDVEAPVDVLLDPRGEDPDACLVRLRDGGLEGERSPWAPLAVT
                     LSSASAGSHPEVASGRLAVVDVAAQAMCELVEPADVVVDLAAAPGGKTRTLLATGRAR
                     RVVALERNPTRARRLAAGLRAAGRAGEVLVVRADSARPPLPSGAFRSVLLDAPCSGTG
                     TLRKNPEIRWRLLPADLRRFAAAQRALLAAALEVCAPGGSVVYVTCSLEPEENEEVVA
                     AVLAERGGASVERPDPSRLPLPLARALRPDGSFRVAPGASNDGFTATVLRRGGGSR"
     gene            complement(5156..6082)
                     /gene="fmt"
                     /locus_tag="P5164_12455"
     CDS             complement(5156..6082)
                     /gene="fmt"
                     /locus_tag="P5164_12455"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRY44745.1"
                     /translation="MRIVYFGSPDFALPSLEGLLAAGHEVALVVSQPAKPAGRSAELS
                     DPPLARRAKELGLPVFQPPTLKDDAAVARLADVRADLFVVVAYGKILAQRVLDLPRLG
                     CVNVHGSVLPRWRGASPVQAALLAGDAESGVSIMRMEAGMDTGPVYAVARGPIGPEED
                     AGALSARLAREGAELLVATLPRIEAGAAAPEPQDDALATLCPKIRREDGQADFSRPAE
                     ELVNRLRAFTPWPGLFAFRAGRRVKLLAARAVAGRESASPGEVLSAGEEVVVACGAGA
                     LALARLQAEGRKPLDAGTFVRGERVAPGERWS"
     gene            complement(6102..>6548)
                     /locus_tag="P5164_12460"
     CDS             complement(6102..>6548)
                     /locus_tag="P5164_12460"
                     /inference="COORDINATES: protein motif:HMM:NF020952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRY44746.1"
                     /translation="GAEALLGQGDMLFLPPGTSYLQRVHGGFVSGDEIKEICRFLKKQ
                     AKPIYDDSVTADRDVVPDANGRGGKAGADDADPMYDQAARLVVRERMASISFLQRRLE
                     IGFSRAGKLIDMMQRDGIVGPPSGGAKSREVLVPSDYYDGVDRQPR"
BASE COUNT          927 a         2244 c         2408 g          969 t
ORIGIN      
        1 ccgaagcccg gcgccttgac ggcgcagacc tggagcgtgc cgcggagctt gttcacgacg
       61 agcgtcgcga gggcctcgcc ctcgatgtcc tcggcgatga tgaggagctg cttgccctgc
      121 ttggcggtct gctcgagcac ggggaggagg tccttcatcg aggagatctt cttctcgaag
      181 atgaggatcc gggcgttctc gaggacggcc tccatcctct cggcgtccgt cacgaagtac
      241 ggggagaggt agccgcggtc gaactgcatc ccctcgacga cctcgagggt cgtctcgagg
      301 cccttggcct cctcgaccgt gatgacgccg tccttgccga ccttgtccat cgcctcggcg
      361 atgatcttgc cgatctcctc gtcgccgttg gccgagatcg tcccgacgaa cgcgatctcc
      421 ttggcgtcga cgctcttctt gagcttgtcg atcgactcga cggacttctt cacggcgatc
      481 tcgatgccgc gcttgagctc catcgggttg tggccggagg tgaccatctt gatcccctcg
      541 cggtagatcg cctgcgcgag gaccgtggcg gtcgtcgtgc cgtcgccggc gatgtcggag
      601 gtcttggagg cgacctcgcg gaccatctgg gcgcccatgt tctcgagcgg ctcgggaagc
      661 tcgatctcct tggcgacggt gacgccgtcc ttggtgatca gcggcgagcc gaacttcttc
      721 tcgatgacga cgttgcggcc cttggggccg agcgtgacct tgacggcgtc ggccagcttg
      781 ttgacgccgc ggaggatgtt ctgacgggcg tcctcgccgt aggtgatgtt cttggcagcc
      841 atttctctaa cctcgcctta cttcgcgtcg acgatggcga ggacttcgtc ctcgcggagg
      901 atgaggtact cgttgtcgtc gatcttgatc tcggtgccgg cgtacttgcc gatgaggatc
      961 cggtcgccgg ccttgacggt cagggccacg cgcttgccgt tgtcgtcgaa cttgccctcg
     1021 ccgacggcct cgaccgtggc ctccatcggc ttctccttgg cggtgtcggg gatgatgatc
     1081 ccgccgcgga cctgctcctt gggctcgatc ctcttcacga ggatgcggtc atagagggga
     1141 cggatcttca ttgggtactc cttctcgggt tccggtttgt cggggcagtt caccccgttt
     1201 ggaagctgcg gtagctgtcg ttagcactcg ccagcggcga gtgctaagaa cctattacct
     1261 gcgcgtctct ttgtcaagtg cgggcggcac ccagggttcg gccgtttccg gtccgccgcc
     1321 agctactcct tcgagaactg cgccaggtgg taggccagga atgccttctg cgacggcgag
     1381 agctccggaa acacaggcag tgcaagagtt tccagagcgg cctgctcggc gactgggaag
     1441 tctccggcct tgccgccgag gttcgcgaag cactcctgga ggtggagcgg cacggggtag
     1501 tagatcgcgc acccgacctt cagctgggtg agacggtctt tcagctcgtc ccgacggccg
     1561 cgggcgaccc gaacgacgaa ctggttgtag acgtggtgcc ggccgggcgc ctcacggggg
     1621 aaggcgatcc ctccctcttc gaacgtccgt ccgccatgct cgaggaagcg ccgctcgatg
     1681 tcggccgcgt tctgccgccg cccttcggcc caggcgtcga ggtgcgggag cttgacgtgc
     1741 agaacgacgg cctgcagcgc gtcgagccgg aagttcccgc cgacgagctt gtggtggtag
     1801 cggctcgtct ccccgtgcat ccggagggcg cggagggtcg ccgccatcgc gtcgtcgttc
     1861 gtcgtcacca tcccgccgtc gccgaagccg ccgaggttct tcgacgggaa gaacgagaag
     1921 gcgccgaagt cgccgagggc gcccgccttc ttccccttgt agcccgctcc gagcgactgg
     1981 caggcgtcct cgatgaccgg gatccccttc gccttcgcga cgtcgaggat cgggtccatg
     2041 tcggcgcact ggccgtagag gtggatcggc tggatcgcct tcgtcctcgt cgtgatcgcc
     2101 ttctccagct tctccgggtc gacgttgtac gtcgccgggt cgatgtcgca gaagaccgga
     2161 cgggccccga ggcggacgac ggcgccggcg gatgcgaaga acgtgtaggg cgaggtcacg
     2221 acgtcgtcgc ccgggccgat gccgagcgcc atcatcgcgg cgagctgcgc gtccgttccc
     2281 gacgacatgc cgatcgcgtg cttcactccg acgtattccg cgatcgcctt ctcgaacgcc
     2341 tcgaccttcg ggccgaggat gaacccctgg ctctcgaaga gctcgtcggt cgccgcgcgg
     2401 atctcgtcgc ggaccgtggc gtactgcttc ttcaggtcga gaagggggac ggggacggcg
     2461 agcggcatcg tcggttccat agcggtcgcg aagcatacgg caagacggcc gctgcgaagc
     2521 ctcccccgcc gccgccgcta ccggatccag tatcgcggcg ccggccgccg gcgcggcttc
     2581 agcagcgcga ggagggcgat gcccgcggcg aagcctccga cgtgcgccca ccaggcgacg
     2641 cccccggcgg cggcgcccgc cttcccggag gcgacgaggg ccgcggagcc ctgccagaac
     2701 tggaggagga accagaggag gaggaagagg aaggccggca cctcgacgag cgggaagagg
     2761 aagaagagcg ggacgagcgt gacgacccgc gcgtgcgggt agaggacgaa gtaggcgccg
     2821 aggacgccgg cgatcgcacc cgacgcgccg accatcggga cgagggagga cggggcgagg
     2881 acgccctgga ggagggcggc gaggacgccg ctcccgacgt agagaaggag gaagcggccg
     2941 tggccgagcg cgtcctcgac gttgtcgccg aagatccaga ggaagagcat gttcccggcg
     3001 aggtgcatca ggccgccgtg caggaacatg gaggcgaaga gcccggcgag gccgagggag
     3061 aagctcccgg gaccgagctc gccggagtgg aagagtcgcg ccgggacgaa cccgaacgcg
     3121 tcgacgagcg cctgccctcc gttcccggcg aggagctgga ggacgaagac gccgacgcac
     3181 gcggcgagga tcgcgctgcg gacgatcggg acggtgcggc tcgggatcgt gtcgcggagc
     3241 ggaatcacgg tccggcgatg ataagggcct ccgcaccgtc gccctctcga aggcggcctt
     3301 caacgcgaaa cgccccgggg gagaccccgg ggcgctgcgg aggcggcgcc tccgctccga
     3361 agggccttcc ggcccctcga gacgatcgcg ggcgggctac ttcctcttct tgttgatgag
     3421 ggtcttgagg tccttgccgg ccttgaactt cgcgaccttc gacgcggcga tcttgatctt
     3481 cgccttcgtc tgcgggttga tgccgacgcg ggccttgcgc tcggcgatgc tgaagatccc
     3541 gagcccgggg acggcgacgc gctcgccctt cttgaggctg tcctggatcg agttcacgaa
     3601 cgcctcgaag gcggacgtcg cgtccttctt cgacagctcg gtttccttgg aaatcgcctc
     3661 gacgatctcg gcctttccag ccatgtgcgg gttcctcctc tttctctctt ctcgtgctgt
     3721 ctccggggga acgccccgga aagcgtcacg tctcttggaa cggcggcaat gtaggatcag
     3781 ctccggacct tgtcaacgga aaacccgcat gttcagcggc tccctccgcc cctccggagg
     3841 acggtggccg tgaacccgtc gttcgaggcg cccggcgcga cccggaagga gccgtccggg
     3901 cggagcgccc gggcgagggg cagaggaaga cgggacgggt cgggccgctc gacggacgcg
     3961 ccgccgcgct cggcgaggac cgccgccacg acctcctcgt tctcctcggg ctcgagggag
     4021 caggtcacgt agacgacgct cccgcccggc gcgcagacct cgagcgcggc ggcgaggaga
     4081 gcgcgctgcg cggcggcgaa gcgccggagg tcggcgggaa gcaggcgcca ccggatctcc
     4141 gggttcttgc ggagcgttcc cgtcccggag cagggcgcgt ccagcaggac cgagcgaaac
     4201 gctccgctcg ggagcggggg ccgggccgag tcggcgcgaa cgacgaggac ctccccggcg
     4261 cgccctgcgg cgcgcaggcc cgccgccagc cggcgcgccc gcgtcgggtt ccgctcgaga
     4321 gcgacgacgc gccgcgcgcg gccggtcgcg agaagcgttc tcgtcttccc gcctggcgcg
     4381 gccgcgaggt cgacgacgac gtccgccggc tcgacgagct cgcacatcgc ctgggcggcg
     4441 acgtcgacga cggcgagccg gcccgaggcg acctccgggt gactcccggc cgaagcggac
     4501 gagagcgtga cggcgagcgg cgcccaggga gaccgctccc cctcgaggcc cccgtcgcga
     4561 agacgcacga ggcacgcgtc ggggtcctcg cccctcgggt ccagcaggac gtccaccggg
     4621 gcctccacgt cgtcggagag cagcgcggcc tcggtcgcct cgaggccgag gtcggcggcc
     4681 cagcgccgga cgagccactc ggggtgggag gtctcgagcg cgagccggac gagcgggtcc
     4741 gtccccgggg ggaggtcgat ccggggaggc ttcccttcgc cggccacctt ccggaggacg
     4801 gcgttcacga ggcccgcggc ccgcggcacg cgaagccgca ccgcgccgac cgtctccccg
     4861 acggccgcgt gaggcgggac gcgctggagg agaaggagct gggcggcccc gacgcgcagg
     4921 cccgccctca cgtcggcctc cagcgtctcg atcggacggg agaggtgccg cgtgaggacg
     4981 tggtcgagga gcgcacggtt ccggagcgcg gagaggacga tcgtccggag gaggccccgg
     5041 tcgcgcggcg gcaggcgccc ctccttcgcg gcgaggagcg gcgcggcgaa ggcgcggtcg
     5101 cgctcgatgc ggacgaggac ctccacggcg agggcgcgga ccggtacgcc gtccgtcacg
     5161 accagcgctc gccgggcgcg acccgctcgc cgcggacgaa cgtcccggcg tcgagcggct
     5221 tgcgcccctc ggcctgcagg cgggcgaggg cgagcgcccc cgcgccgcag gcgacgacga
     5281 cctcctcgcc cgccgagagg acctcgccgg gcgaggcgct ctcgcgcccc gcgacggcgc
     5341 gcgccgcgag gagcttcacg cgccggccgg ctcggaacgc gaagagcccg ggccacggcg
     5401 tgaaggcgcg aaggcggttc acgagctcct ccgcggggcg ggagaagtcg gcctggccgt
     5461 cctcgcggcg gatcttcggg cagagcgtcg cgagggcgtc gtcctgcggc tcgggggccg
     5521 ccgcgccggc ctcgatccgc gggagcgtcg cgacgaggag ctccgcccct tcccgcgcga
     5581 ggcgcgccga gagggcgccc gcgtcctcct ccgggccgat cggaccgcgc gcgacggcgt
     5641 agaccggtcc ggtgtccatc cccgcctcca tccgcatgat cgagacgccg ctctccgcgt
     5701 ctccggcgag gagcgccgcc tggacgggcg acgcgccccg ccagcgcggg aggaccgagc
     5761 cgtggacgtt cacgcagccg aggcgcggca ggtcgaggac cctctgcgcg aggatcttcc
     5821 cgtaggcgac gacgacgaag aggtcggcgc ggacgtccgc gaggcgcgcg accgcggcgt
     5881 cgtccttcag cgtcggcggc tggaagacgg ggaggccgag ctccttcgcg cgcctcgcga
     5941 gcggagggtc ggagagctcc gcagagcggc ccgcgggctt cgccggctgg gagacgacga
     6001 gggcgacctc gtgccccgcg gcgaggagcc cctcgagcga cgggagggcg aagtcgggcg
     6061 agccgaagta gacgatgcgc atcggagccg ccgtcggacc gtcagcgcgg ctggcggtcg
     6121 acgccgtcgt agtagtcgga ggggacgagc acctcgcggc tcttcgcgcc gcccgacggc
     6181 gggccgacga tcccgtcgcg ctgcatcatg tcgatgagct tccccgcgcg ggagaagccg
     6241 atctcgagcc gcctctggag gaacgagatc gaggccatcc tctcgcggac gacgaggcgc
     6301 gccgcctggt cgtacatcgg gtcggcgtcg tcggcgcccg ccttgccgcc ccggccgttc
     6361 gcgtcgggga cgacgtcgcg gtctgcggtg acggagtcgt cgtagatcgg cttcgcctgc
     6421 ttcttcagga agcggcagat ctccttgatc tcgtcgcccg acacgaaccc gccgtggacc
     6481 cgctgcagat aggacgtccc cggcgggagg aagagcatgt cgccctggcc gaggagcgcc
     6541 tcggcccc
//
LOCUS       DAOTEZ010000230         6527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_8447_length_6527_cov_5.244747, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000230 DAOTEZ010000000
VERSION     DAOTEZ010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8447_length_6527_cov_5.244747"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1714)
                     /locus_tag="P5164_12465"
     CDS             complement(<1..1714)
                     /locus_tag="P5164_12465"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRY44747.1"
                     /translation="MRGRLADGVYDHLLDDALKALVGDVGPGREAAIADLEPGRTPSF
                     VAAHLKPLVERVLASVQGDPAEKQRRRFEILNRLLTTLAQAAHEHCAESPFGEGELRV
                     DGPRHLRLVRPRPALGALPPVVLPETPLAESALLVNASGEPSLASELARELATADRVD
                     LLCAFVKWSGLRVLLEALQAVVERGREHGIAAPVRVLTTTYIGASEVRALEALAESGA
                     EVRISYDDRRTRLHAKAWLFHRESGSHTAYIGSSNLSRAALHEGLEWNVRLSWRDSAS
                     LLSKFRAAFESYWGADEFEPYEPGRDRERVVRALAAQRTSGGDRGPALRLDLAPHPHQ
                     EPMLEALRVEREHGHWRNLVVAPTGTGKTLVAAFDYRELRAKLPRARLLFVAHRERIL
                     TQSRDAFAAVLRDPGFGELLTGEHEAARGEHVFATIQSLSRRMFAEELPPEHYDVVIV
                     DEFHHAEAPTYERLLEHVRPGVLLGLTATPERFDGKDVRRWFDGRTAYEMRLWEALEQ
                     GLLAPFQYFGVKDSVDLDDVAWNRGWGYDRGELARRYVVAGGAEARARLVLQEVERRV
                     ADPRR"
     gene            complement(1977..2402)
                     /locus_tag="P5164_12470"
     CDS             complement(1977..2402)
                     /locus_tag="P5164_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44748.1"
                     /translation="MSGVEAHRDPFELQVSGEKLAPESDESLGEVEVPTFPSLAKPRM
                     PSAFQGDPNVPGADRDAQGFVLREVPGGIEADDEPTGGVDAEEDVGEETEGISPAGWN
                     VAGVREADRRARPSSPGAGGAQAEQEAAEARRPAEGILE"
     gene            complement(2720..4174)
                     /locus_tag="P5164_12475"
     CDS             complement(2720..4174)
                     /locus_tag="P5164_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HRY44749.1"
                     /translation="MPTARERLALWGRTIFRVLGQNRLSQAGVVVTTATGISMVFLWV
                     IELLRGGHGHPYSGIILFLILPGLFVAGLLLIPAGYFLQRWRDKKAGRAVEPPPPVDF
                     GSPKVRRVATFVVAATLLNATIVGFASYKGLEVMDSVEFCGEACHTVMAPELAAYRGS
                     PHSRIGCVECHIGEGASWFVKSKLSGTRQVVAVALKTYSKPIPSPVHHLRPARETCEQ
                     CHWPQRFTGDRVVIKKKYAEDEKSTELTTVLVMHVGGHNAQGTVGIHGRHLDAGSRIS
                     YTAIDGRRQEIARVTWKDDTGKEVEFASTEVKATPEQLAKGETRAMDCVDCHNRPTHA
                     FELPHRGLDREITAGSISRDLPFVKKKALELLKVKYTSQEEASKAIEAGLRAFYASEH
                     PDVAAAKKAELDRAVKAVQSVYLRNVFPNMDLDWGYHPNNIGHEDFPGCFRCHNGNLE
                     AKDGTTVSADCEACHQVLAMEEENPEILTQLGLK"
     gene            4354..6156
                     /locus_tag="P5164_12480"
     CDS             4354..6156
                     /locus_tag="P5164_12480"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRY44750.1"
                     /translation="MARNGRDQTPARPDRKRLLYFAPSRAHADSLLGALTGQPGATRC
                     REDGSTVLHLGGTAFRFVLRQDPVEAHEALHREYFNGVLLDLRAVPGPASRLEECFAR
                     SLRLLDLMDAEPDPELRFGFHRILALLSGPDPVVVDDRIRLLGARGVGRVLRDPSPGA
                     GDRRGPRLPASDALGRLVGDELVRMTTGRRVGSRALCASGGGITGIYFEMGALKCLAD
                     CLPPGALNAFDMYFGISAGAVLTGILANGYSVDEFMASIAGLGCERIPPVDLSLLKVS
                     NLNPASLAAPLETVGRALAAGLADLVRGRISPSLSSLFLDYSDFLAAPFRADGYEAML
                     RYLFTRPGATNDFRKLQRRLFVGATDQDAREHVLFGEAGLEDVPISLAIQASLSINPA
                     FSSTRIGERYYVDGAVTRTSDFTEAIRRGADLIFVLDPLVPYVSKGAAGFASRRGVLY
                     NADQDIRTVSYTRFETTRRWVLRQHPEVSLYAFLPANRQRKVLSVNPMDHRPFLAVWR
                     GAYLSTLQRIHLLRHKMCGDLAAHGLALDTAKAEAVAARLEATQEPSFADFFPDGKVA
                     VAKGMKPGRALRLAGAPPAEEVVCRAGDRSPAVA"
     gene            complement(6161..>6527)
                     /locus_tag="P5164_12485"
     CDS             complement(6161..>6527)
                     /locus_tag="P5164_12485"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRY44751.1"
                     /translation="ETPTPEPTPTPTEVPTPTAAPALPQGAIPPVRSSDVRPVFDEEL
                     MRRHTGLSGQVELEVLVNADGSVGSIRVVRGVAPEVDAIVAEAAREQLAYSPATFRGK
                     PIAAKSRVVVGVRFLVAPR"
BASE COUNT          868 a         2424 c         2234 g         1001 t
ORIGIN      
        1 ttcgccgcgg gtcggcgacg cgccgctcga cctcctgcag gacgagccgc gcccgcgcct
       61 ccgcgccgcc ggccacgacg tagcgccgcg ccagctctcc cctgtcgtag ccccacccgc
      121 gattccacgc cacgtcgtcg aggtcgaccg agtccttcac tccgaagtac tggaagggtg
      181 cgaggagccc ctgctcgagg gcctcccaga ggcgcatctc gtacgccgtc cgcccgtcga
      241 accagcggcg cacgtccttc ccgtcgaagc gctccggcgt cgccgtcagg ccgaggagga
      301 caccgggccg cacgtgctcg aggagccgct cgtacgtcgg cgcctcggcg tggtggaact
      361 cgtcgacgat gacgacgtcg tagtgctccg gcggcagctc ctccgcgaac atcctccgcg
      421 agagcgactg gatcgtcgcg aagacgtgct ctccccgcgc ggcctcgtgc tcgcccgtca
      481 gcagctcgcc gaaaccgggg tctctcagga cggccgcgaa ggcgtcgcgg ctctgcgtca
      541 ggatccgctc gcggtgggcg acgaagagga gcctcgcccg cggcagcttc gcccgcagct
      601 cgcggtagtc gaaggccgcc acgagcgtct tgccggtccc cgtgggcgcg acgacgaggt
      661 ttctccagtg cccgtgctcc cgctcgaccc gcagcgcctc cagcatcggc tcctggtgcg
      721 ggtggggcgc gaggtcgagc ctcagcgccg ggccccgatc cccaccggac gtcctctgcg
      781 ccgccagcgc ccgcacgacc ctctcgcggt cgcggcccgg ctcgtacggc tcgaactcgt
      841 cggcgcccca gtacgactcg aacgccgccc ggaacttcga gagcagggac gccgagtcac
      901 gccaggagag ccggacgttc cactcgagcc cctcgtggag cgcggcgcga gagaggttcg
      961 aggagccgat gtaggccgtg tggctgccgc tctcccggtg gaagagccac gccttcgcgt
     1021 ggaggcgcgt ccggcggtcg tcgtacgaga tccgcacctc ggctccgctc tccgcgagcg
     1081 cctcgagcgc ccgcacctcg ctcgcgccga tgtacgtcgt cgtcaggacg cgcaccggcg
     1141 ccgcgatgcc gtgctcccgc cctcgctcga cgacggcctg aagcgcctcg agcagcaccc
     1201 gcaagccgct ccacttcacg aacgcgcaga ggaggtccac ccggtcggcc gtcgccagct
     1261 cccgcgcgag ctcgctcgcg agcgacggct cgccggaggc gttcacgagg agcgcgctct
     1321 cggcgagcgg cgtctcggga aggaccaccg gaggaagggc cccgagcgca ggccggggcc
     1381 gaacgaggcg caggtgccgc ggcccgtcga cgcggagttc tccttcgccg aacggcgact
     1441 cggcgcagtg ctcgtgcgcc gcctgagcaa gggtcgtgag aagccggttc aggatctcga
     1501 accggcgtcg ctgtttctcg gccggatcgc cctgcaccga agcgaggact cgttcgacga
     1561 gcggcttcag gtgcgccgcg acgaacgacg gcgtccgccc cggctcgagg tccgcgatcg
     1621 ccgcctcgcg tcctggaccg acatccccga cgagcgcctt cagcgcgtcg tcgagaaggt
     1681 ggtcgtagac gccgtcggcg agccgccccc gcacgtcgcc agggtactcg agcagacgcc
     1741 cgggccgcca acagaacagg ctacggaccc gtcccgtcgc gtcccgctgc ctcgacagcc
     1801 cgggggagct ccattcctgg cgcgagctcg ataccctcga gaggatggcg aggacccgat
     1861 tctgtccgtt cgttcccgag ggctcgccgc tcctcgacca tctctgcggc tcgctctctc
     1921 gctccggctg cagcctgctt cgggtcgaag ggacggacca ggtcggcttc cgtctctcat
     1981 tcgaggatcc cctctgcagg ccttcgggct tcagcggctt cctgttccgc ctgggctccg
     2041 ccggcccccg gcgaactcgg gcgggcacga cggtctgctt cccggactcc cgcgacgttc
     2101 cacccggccg gcgagatccc ttcggtctct tcacccacgt cctcctcggc gtcgaccccg
     2161 cccgtgggct catcgtcggc ttcgatccct cccggcactt cccggaggac gaaaccctgc
     2221 gcgtctcgat cagccccagg gacattcggg tcaccctgga acgcggatgg cattcgtggc
     2281 ttcgcgaggg atgggaacgt cgggacttcg acttcgccga ggctctcgtc ggattccgga
     2341 gcgagcttct ccccgcttac ctggagttcg aacggatcgc ggtgggcctc gacaccggac
     2401 accgcagcct tctcgcggac acgttcttcg acgccacacg gaggcgcagc cgccgaacgt
     2461 gacgcccccg cacgcgtcgt cgctcccggc tggtccaccg ccccgcaagc ctgtcgacga
     2521 cccgctcggg cgcgaatcac gaggccgggc ggtcgcgctt ccccgggaat cggccctctc
     2581 accttcgtgc gacggggagg gagccggctc gggactcccg gtccgaagcg tcggccccgc
     2641 ccgctgcatg ggcactgggc ggggccgcgt cgtctgtggc aggcgccggc catcgcgccg
     2701 acgcctgccc ttccggtccc tacttcagcc cgagctgcgt gaggatctcc gggttctcct
     2761 cttccatcgc gaggacctgg tggcacgcct cgcagtcggc ggagaccgtc gtcccgtcct
     2821 tcgcctcgag gttcccgttg tggcagcgga agcagccggg gaagtcctcg tgcccgatgt
     2881 tgttcgggtg gtagccccag tcgaggtcca tgttcgggaa gacgttccgg aggtagacgg
     2941 actggacggc cttcacggcc cggtccagct cggccttctt cgcggccgcg acgtccgggt
     3001 gctcggaggc gtagaaggcc cggaggcccg cctcgatcgc cttcgacgcc tcctcctggg
     3061 aggtgtactt caccttcagg agctcgaggg ccttcttctt gacgaacggc aggtcgcggc
     3121 tgatcgaccc ggcggtgatc tcgcgatcga ggccgcggtg cgggagctcg aaggcgtggg
     3181 tcgggcggtt gtggcagtcg acgcagtcca tcgcccgggt ctcgcccttg gcgagctgct
     3241 cgggggtcgc cttcacctcg gtgctcgcga actccacttc cttcccggtg tcgtccttcc
     3301 aggtgacgcg ggcgatctcc tggcggcgcc cgtcgatcgc cgtgtagctg atgcgcgagc
     3361 cggcatcgag gtggcggccg tggatgccga cggtcccctg cgcgttgtgc cctccgacgt
     3421 gcatgacgag aacggtcgtc agctcggtgc tcttctcgtc ctccgcgtac ttcttcttga
     3481 tgacgacgcg gtccccggtg aagcgctgcg gccagtggca ctgctcgcac gtctcgcgcg
     3541 ccggccggag gtggtggacc ggcgacggga tcggcttcga gtaggtcttg agggcgaccg
     3601 cgacgacctg tctcgtgccg gagagcttcg acttcacgaa ccaggaggcc ccttcgccga
     3661 tgtggcattc gacgcagccg atgcgggagt gcggcgagcc gcggtaggcc gcgagctccg
     3721 gggccatcac ggtgtggcag gcctcgccgc agaactcgac ggagtccatc acctcgagcc
     3781 ccttgtaaga ggcgaagccg acgatggtcg cgttcaggag agtcgcggcg acgacgaacg
     3841 tcgccacgcg gcggaccttc ggagagccga agtcgaccgg cggaggcggc tcgacagccc
     3901 ggccggcctt cttgtcgcgc cagcgctgca ggaaatatcc cgcggggatc aggaggagcc
     3961 cggccacgaa gaggccgggc aggatcagga agaggatgat cccggagtac gggtgcccgt
     4021 gtccgccgcg gaggagctcg atcacccaca ggaacaccat cgagatcccg gtcgccgtcg
     4081 tgacgacgac ccccgcctgg ctgagccggt tctgtccgag gaccctgaag atcgtccgcc
     4141 cccaaagggc gagtcgttcc cgcgcggtcg gcatctggcc tctcctcctc cgccgtagtt
     4201 tcacacggga cgtcccggag accggcccgt cccgccgtgc cgccacggcc cgcgccccgg
     4261 gaggacagtc ccgcttgacg cctcgcggca aaaggcggag attgccggca gggcggaacg
     4321 agcggccgcc gcggcacggc gagggggtgc agcgtggcga gaaacgggcg ggaccagacc
     4381 ccggcgcggc cggaccgcaa gaggctgctc tacttcgccc cgagccgcgc ccacgccgac
     4441 agccttctcg gcgcgctcac ggggcagccc ggcgcgacgc gatgccgcga agacgggtcg
     4501 acggtccttc atctgggcgg caccgccttc cgtttcgtcc tccgtcagga ccccgtggag
     4561 gctcacgagg ccctccaccg cgagtacttc aacggggttc tcctcgacct tcgggcggtg
     4621 ccgggacccg cgtcccgcct cgaggagtgc ttcgcgcgct ccctccgcct cctcgacctg
     4681 atggacgccg agccggaccc ggagcttcgc ttcggcttcc accgcatcct cgccctcctc
     4741 tccggccccg atcccgtcgt cgtggacgac cggatccggc tgctcggcgc ccggggtgtc
     4801 gggagggtcc tccgcgaccc gtcgcccggg gcaggcgatc gccgcggacc tcgcctcccg
     4861 gcctcggacg cgctcggccg cctcgtgggc gacgagctcg tccggatgac gacgggccgc
     4921 cgggtcggct cgcgggccct ctgcgcttcc ggcggcggga tcacggggat ctacttcgag
     4981 atgggcgcgc tgaagtgcct cgccgactgc ctgccgcccg gcgcgctgaa cgcgttcgac
     5041 atgtacttcg ggatcagcgc cggagccgtc ctgaccggga tcctcgcgaa cggctactcg
     5101 gtcgacgagt tcatggcgtc gatcgccggc ctcggctgcg agcggattcc tccggtggac
     5161 ctctcgctcc tcaaagtgtc gaacctcaac cccgcgagcc tcgcagcgcc cctggagacg
     5221 gtcgggcggg cgctcgcggc ggggctcgcc gatctcgtcc gcgggaggat cagcccgtcg
     5281 ctgtcgtctc tgttcctcga ctactcggac ttcctcgccg ctccgttccg cgccgacggc
     5341 tacgaggcga tgctccggta cctcttcacg cggcccggag cgacgaacga cttccgcaag
     5401 ctgcagcgcc gcctcttcgt cggagcgacc gaccaggacg cccgcgagca cgtcctcttc
     5461 ggcgaggccg gcctcgaaga cgtgccgatc agcctcgcga tccaggcgtc gcttagcatc
     5521 aacccggctt tctcgtcgac gaggatcggg gagcgctact acgtcgacgg cgccgtcacg
     5581 cggacctccg acttcaccga ggcgatccgg aggggggcgg acctgatctt cgtcctcgat
     5641 ccgctcgtcc cgtacgtctc gaagggggcc gccggcttcg cgagccgccg cggggtcctc
     5701 tacaacgccg accaggacat ccggaccgtc tcgtacacgc gcttcgagac gacgcggcgc
     5761 tgggtcctcc ggcagcaccc ggaagtgagc ctctacgcgt tcctcccggc gaaccgccag
     5821 cgaaaggtcc tctcggtgaa cccgatggac caccgtccgt tcctggcggt ctggcgcggc
     5881 gcctacctct ccacgctcca gcgaatccac ctccttcgcc acaagatgtg cggcgacctg
     5941 gccgcgcacg gcctcgcgct cgacacggcg aaggcggagg cggtggccgc gcggctggag
     6001 gcgacccagg agccttcctt cgccgacttc ttcccagacg gaaaggtcgc cgtcgcgaag
     6061 ggcatgaagc cgggccgcgc gctccgtctc gccggcgcgc ctcccgccga ggaggtcgtc
     6121 tgtcgggcgg gagaccggag cccggccgtc gcctgagcgg tcagcgcggc gcgacgagga
     6181 agcggacgcc gaccacgacg cggctcttcg ccgcgatcgg cttccctcgg aacgtggccg
     6241 gtgagtacgc gagctgctcg cgggccgctt cggcgacgat cgcgtcgacc tccggggcga
     6301 cccctcggac gacgcggatc gatccgacgc ttccgtccgc gttcaccagg acctcgagct
     6361 cgacctggcc gctcagcccg gtgtggcgcc gcatcagctc ttcgtcgaag accggtcgga
     6421 cgtccgagga gcggaccggc gggatcgcgc cctggggaag cgcaggagct gcggtcggcg
     6481 tcgggacctc ggtcggagtc ggggtcggct cgggcgtcgg cgtctcg
//
LOCUS       DAOTEZ010000231         6515 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_8468_length_6515_cov_5.697368, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000231 DAOTEZ010000000
VERSION     DAOTEZ010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6515
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8468_length_6515_cov_5.697368"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1560
                     /locus_tag="P5164_12490"
     CDS             <1..1560
                     /locus_tag="P5164_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006458084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRY44752.1"
                     /translation="DKRASLPWVGDVGALFGKVERHDRDGFYGPCDPGRGTRLEKGLL
                     PPELIIQDELHLISGPLGTIAGLYEIAVDRLSSREEAGRRIPPKVVASTATIRRAEAQ
                     VRALFGRSAVYVFPPPGPDRRDSFFARTVSQSEKEPRTYVGLSAQGRSLKVLLLRTYL
                     ALLGASQRLFEETGGAALGEKNPADPYLTLLGYFSSLRELGGTRRIVEDEVAAQAARR
                     SERRRPSAGRGEFHDRAIQQEVVELTSRESTAKVARAKERLALPFTEKEHVDVALATN
                     MISVGLDITRLGLMVVLGQPKTTSEYIQATSRVGRDDARPGLVVTLQNVHRPRDRSHY
                     ERFGAYHASFYRAVEATSVTPFSARALERAAAAVAVSLARHGMPVLAPAAGAGEVEAV
                     RKDLAYVAEEMAERAMRHDPQKSAEELEALRKKVKSLVDDLLDGWAALAHEKKQNGVK
                     LAYQAKERGAPPNAFALLRDPLDPELDSLPSQARKFRAQRSMRDVEPSVGLVLRRLDN
                     AAVPDAEEAES"
     gene            1557..3362
                     /locus_tag="P5164_12495"
     CDS             1557..3362
                     /locus_tag="P5164_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011316684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HRY44753.1"
                     /translation="MKKRHHGEIRRSQLVRTFGPGAMVDLPSHSVVVSGLDSWFPAGA
                     MPAIVEERLSLKVASVLGHGKPVPLKGPPVVSDDERTPATGVPAFQFPEWFVVQAKNK
                     RRPLVHLDALDKGKYVVDGKKVPAVPVRFVQACIRGHLSDIDWRGFIHMYRGDCVRPI
                     WMEERGSSGDLADVIVGCDCGAERSMAQATRRGAENLGTCPGKRPWLGRFGGEKCGGE
                     GGKLELARLLVRSASDAYFPQVLSVISLPDRGSVVRDAVAKVWENFLSLVEEPGELAK
                     ERKRPAVKAALESITDDEVWAEILRRKSGAPAKSEKIKEVELATLLSVKEKLGDEFHG
                     GHDFLARRLPLEAPTGPMKAIERVVLVDRLREVTAQVGFTRFEAQTADIDGELSLDVR
                     SAPLAAEVKWVPAVENRGEGVFLTLDPACVAAWLERPGVKERGKQLALGFERWKTAHK
                     KPAMPFPGLPYVLLHSFSHLLLTAVSLDCGYATSSIRERIYVGEGAFGLLLYTSSPDA
                     EGTLGGLVEVGRNLGRHLGRALELARLCSNDPVCAGHRPDSGHEERFLAGAACHGCLL
                     VSEPSCENRNDFLDRALVVPTVLGGGAEFFAEDEP"
     gene            3359..4150
                     /gene="drmC"
                     /locus_tag="P5164_12500"
     CDS             3359..4150
                     /gene="drmC"
                     /locus_tag="P5164_12500"
                     /inference="COORDINATES: protein motif:HMM:NF038319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DISARM system phospholipase D-like protein DrmC"
                     /protein_id="HRY44754.1"
                     /translation="MSGPWEGLSRGGLEALAGALAAGRLSGPYSASGLARHLPYVELA
                     GALAELNRLHDAGVPPAHVAEVLLAVAAERKLREKTSDRVELVWTGPEMPGSQSRETG
                     VVARELFAKATRSVLVSSYAVFLGKEVFEPLAKRMEELPELRVLLFLNVPAPGGSDFD
                     PDAVARDYFREFLKRNWPSGRAPELYYDPRSLAAKGDERAVIHAKCVVVDGHTALVTS
                     ANFTEAAHARNIEAGAVVHDAGFAGSLERQFTSLVTAGLLRRVVV"
     gene            4228..5103
                     /locus_tag="P5164_12505"
     CDS             4228..5103
                     /locus_tag="P5164_12505"
                     /inference="COORDINATES: protein motif:HMM:NF021361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HRY44755.1"
                     /translation="MRLSLLAAVAVVATLPLGAVEPEPSKRQRELIVELLGLTAGEGT
                     MHEVMNGILEQSEKQFIAKAEARGEVPKDVEERRKLYASLHEELARIDVSAALAEPAI
                     RIYAKYYTERELADLVAFYRTPTGKKSIKVLPAIVRESAEASAKSLGPLLEEAAARAW
                     HRQEPEERPWKKAMEDIFTLARAILDYSSGTRDSSYPPGDFASLGRVLVPKHLGSMPA
                     KDPWGTPYGYVASPDGQHYRLVSAGADRKFEPESLRIVVTANGRPPMDRASQRLEDDI
                     IQQDGVFVQYYAPKR"
     gene            complement(5200..>6515)
                     /locus_tag="P5164_12510"
     CDS             complement(5200..>6515)
                     /locus_tag="P5164_12510"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRY44756.1"
                     /translation="DPMSVITIRHLLSMTSGLTHDAPVGNNADPYTPSYEDHIRSISR
                     TWLRFRTGERAEYSNLGVELAAYILESITHRPFPEYVQRKVLDPLGMRRTTWDIDRIR
                     KDGNRAVGNNRSFESVPLENPMLAPGGAYSTIGDMAVFLRFQMNDGRVDGRPLIDERV
                     LRQMRTIPFPMKDQVAGYGMGLWVGAYHLGGDDVLWLAHGGGGFGFRCQMKWLPDLGY
                     GVVVLTNAQDHDNVNDALAEDLLLKVVELLTGRKDQGPSDWLARHTPPRTADPSSLPV
                     ELAGRYCGTNDDMTFLVKDGTFGYASGNTFMPITPISKYEYATKRYLYRFVCDTGGKP
                     VSVVRPYDGTVWTMGSPASEPKGPDRKEWSVYVGSYVRKRFGVGEKFYNVSVRNGWLH
                     FQGDGQDFRLSEHAPGLFFTPDGEAVDLRGPAFSFRNIRLYKTCG"
BASE COUNT         1014 a         2155 c         2387 g          959 t
ORIGIN      
        1 gacaagcgcg cgagcctgcc gtgggtgggc gacgtgggcg cactcttcgg aaaggtggag
       61 cggcacgaca gggacggctt ctacgggccg tgcgaccccg gccgggggac gcggctcgag
      121 aaggggctcc tgccgccgga gctgatcatc caggacgagc tgcacctcat ctcggggccg
      181 ctcgggacga tcgccgggct ctacgagatc gccgtggacc ggctctcctc gcgcgaggag
      241 gcggggaggc ggattccgcc gaaggtggtc gcctcgacgg cgacgatccg ccgggccgag
      301 gcgcaggtgc gcgcgctctt cggccggagc gcggtctatg tcttcccgcc cccggggccg
      361 gaccggcgcg actcgttctt cgcgcgtacg gtttcgcaat ccgagaagga gccgcggacg
      421 tatgtcggcc tctcggcgca ggggcggagc ctgaaggtgc ttctgctgcg gacgtacctc
      481 gcgctgctcg gtgcctcgca gcgtctgttc gaggaaacgg gcggtgcggc cctcggcgag
      541 aagaacccgg ccgacccgta cctgacgctc ctcggctact tctcgagcct tcgagagctg
      601 ggaggcacgc ggcggatcgt agaggacgag gtggccgcgc aggcggcgcg ccggtcggaa
      661 agacgtcggc cgagcgctgg cagaggggag ttccacgatc gggccatcca gcaggaggtc
      721 gtggagctga cgtcgcggga gtcgacggcg aaggtggccc gggcaaagga acggctggcg
      781 ttgccgttca ccgaaaagga gcacgtcgac gtcgcgctcg cgacgaacat gatctcggtc
      841 ggcctcgaca tcacgcggct cgggctgatg gtcgtcctcg ggcagccgaa gacgacgtcg
      901 gagtacatcc aggcgacgag ccgcgtcggg cgcgacgacg cgcggcccgg gctcgtcgtg
      961 acgctccaga acgtccaccg cccgcgcgac cgatcgcact acgagcggtt cggggcgtac
     1021 cacgcgtcgt tctaccgggc cgtggaggcg acgagcgtga cgccgttctc ggcccgcgcc
     1081 ctcgaaaggg ccgcggccgc cgtggcggtt tcgctcgcgc ggcacgggat gcccgtgctc
     1141 gcgcctgcgg cgggggccgg ggaagtggag gccgtgcgga aggacctcgc ctacgtcgcc
     1201 gaggagatgg cggagcgggc gatgcggcac gacccgcaga agagcgccga ggagctggag
     1261 gccctccgga agaaggtgaa gagcctcgtg gacgaccttc tcgacggctg ggccgccctg
     1321 gcacacgaga agaagcagaa cggcgtgaag ctcgcctacc aggcgaagga gcgcggcgcc
     1381 ccgccgaacg ccttcgcgct cctccgcgac ccgctcgacc cggagctcga ttcgctcccg
     1441 tcgcaggccc gcaagttccg ggcgcagcgc tcgatgagag acgtggagcc gagcgtcggg
     1501 ctcgtcctcc ggcggctcga caacgccgcg gtgccggacg ccgaggaggc cgaatcgtga
     1561 agaagaggca ccacggcgag atccgccgca gccagctcgt gaggacgttc gggccgggcg
     1621 cgatggtcga cctcccgagc cactccgtcg tcgtgagcgg gctcgactcc tggtttccgg
     1681 cgggagcgat gccggcgatc gtcgaggaaa ggctgtcgct gaaggtcgcg agcgtcctcg
     1741 ggcacgggaa gcccgtgccg ctgaagggcc ctccggtcgt ctccgacgac gagcggacgc
     1801 cggcgacggg cgtcccggcg ttccagttcc cggagtggtt cgtggtgcag gcgaagaaca
     1861 agcggaggcc gctcgtccac ctcgacgccc tcgacaaggg gaagtacgtc gtcgatggga
     1921 agaaggtccc ggccgtcccg gtccggttcg tccaggcatg catccgcggc cacctctccg
     1981 acatcgactg gcgcggtttc atccacatgt accggggcga ctgcgtgcgg ccgatctgga
     2041 tggaagagcg cggatcgtcg ggagacctcg cggacgtgat cgtcggctgc gactgcgggg
     2101 cggagcgctc gatggcgcag gcgacgcgcc gcggcgccga gaacctcggg acgtgtccgg
     2161 gaaagcgccc gtggctcggg aggttcggcg gtgagaagtg cgggggcgag ggagggaagc
     2221 tcgagctggc ccggctcctc gttcgatcgg cgagcgacgc gtacttcccc caggtgctga
     2281 gcgtcatctc gctcccggac cgcggctcgg tcgtccgaga cgcggtggcg aaggtctggg
     2341 agaacttcct ttcgctcgtc gaggagccgg gtgagctggc aaaggaacga aagcggcccg
     2401 ccgtgaaggc cgcgctcgaa tccatcaccg acgacgaggt ctgggccgag atcctgaggc
     2461 ggaagtcggg cgcgcccgcg aagtccgaga agatcaagga ggtcgagctg gcgacgctcc
     2521 tctccgtgaa ggagaagctg ggcgacgagt tccacggggg gcacgacttc ctggcgcgcc
     2581 ggctgcctct cgaggcgccg accgggccga tgaaggcgat cgagcgcgtc gtcctcgtcg
     2641 accggctccg cgaggtgacc gcgcaggtcg gcttcacgcg gttcgaggcg cagacggccg
     2701 acatcgacgg ggagctgtcg ctcgacgtga gatcggcgcc cctcgcggcc gaggtgaagt
     2761 gggtgccggc cgtcgagaac cggggcgagg gggtcttcct caccctcgac cccgcgtgcg
     2821 ttgccgcgtg gctcgagcgg ccgggcgtga aggagcgagg gaagcagctc gcactcggct
     2881 tcgagcggtg gaagacggct cacaagaagc ccgcgatgcc gttcccgggg ctgccctacg
     2941 tccttctcca ctcgttctcg cacctcctgc tcacggccgt ctcgctcgac tgcggctacg
     3001 cgacgagctc gatccgcgag cggatctacg tgggcgaagg ggccttcggg ctccttctct
     3061 acacctcgag ccccgacgcg gaagggacgc tcggcggcct cgtggaggtg ggccggaacc
     3121 tcggccggca cctcgggcgc gcgctggagc tcgcgcggct ctgctcgaac gaccccgtct
     3181 gcgcgggcca ccggcccgac agcgggcacg aggagcggtt cctcgccgga gcggcctgcc
     3241 acggctgcct cctcgtctcc gagccgagct gcgagaaccg caacgacttc ctcgaccgcg
     3301 ccctcgtcgt accgacagtg ctcggcgggg gagccgaatt cttcgcggag gacgagccat
     3361 gagcgggccg tgggaagggc tctccagggg cgggctcgag gcgctcgcgg gcgcgctcgc
     3421 tgcggggcgg ctctccgggc cgtattccgc gagcggtctg gcccggcatc tcccgtacgt
     3481 cgagctcgca ggcgcgctcg ccgagctgaa ccgcctgcac gacgcgggcg ttccgcccgc
     3541 gcacgtcgcc gaggtcctcc tggccgtggc cgcggaacgc aagctccggg agaagacgtc
     3601 cgatcgcgtc gagctcgtct ggacggggcc ggagatgccc gggagccaga gccgcgaaac
     3661 cggcgtcgtc gcgcgggagc tcttcgcgaa ggcgacgcgg agcgtcctcg tctcgagcta
     3721 tgccgtcttc ctcgggaagg aggtcttcga gccgctcgcg aagcggatgg aggagcttcc
     3781 cgagcttcgc gtcctcctct tcctgaacgt gccggcgccg ggcggaagcg acttcgaccc
     3841 cgacgcggtc gcccgcgact acttccgaga gttcctgaag aggaactggc cgtcgggccg
     3901 cgcgcccgag ctctactacg acccgcgctc gctcgcggcg aagggcgacg agcgggccgt
     3961 catccacgcc aagtgcgtcg tcgtcgacgg ccacaccgcg ctcgtcacct ccgcgaactt
     4021 caccgaggcg gcccatgccc ggaacatcga ggccggcgcc gtcgtccacg acgccggctt
     4081 cgccggctcg ctcgagaggc agttcacgtc gctcgtgaca gcgggattgc tgaggcgagt
     4141 cgtcgtctga ggacgctcgt tcctgacccc gccctcgcgt agtagcattt ctgcaaaacc
     4201 cgacgaacct gaagagcgag gatcgacatg cgcctcagcc tccttgccgc cgttgccgtc
     4261 gtcgccacgc ttccgctcgg cgccgtcgag cccgagccca gcaagcgtca gcgcgagctg
     4321 atcgtggagc tcctcgggct gaccgccggt gagggcacga tgcacgaggt catgaacggc
     4381 attctcgagc agagcgagaa gcagttcatc gcgaaggcgg aggcgcgggg cgaggtaccg
     4441 aaggacgtcg aggagagaag gaagctctac gcctccctgc acgaggagct cgcgcgcatc
     4501 gacgtctcgg cggcgctggc ggagccggcg atacggatct acgcgaagta ctacaccgag
     4561 cgggagctcg cggacctcgt cgccttctac cggacgccga ccgggaagaa gtcgatcaag
     4621 gtgctccccg cgatcgtgcg ggagtcggcc gaggcgagcg cgaagagcct cggaccgctg
     4681 ctcgaggaag cggccgcccg ggcctggcac cgccaggaac cggaggagag gccgtggaag
     4741 aaagcgatgg aggacatctt cacgctcgca cgggcgatcc tggactattc gagcgggact
     4801 cgcgattcga gctatccgcc gggcgacttc gcctcactcg ggcgcgtcct cgtgccgaag
     4861 cacctcgggt cgatgccggc gaaggacccg tgggggacgc cgtacggtta cgtcgcctcg
     4921 ccggacggcc agcactatcg gctcgtgagc gccggcgccg accggaaatt cgagccggag
     4981 agcctgcgca tcgtcgtcac cgccaacggg cggcctccga tggacagggc ctcccagcgc
     5041 ctcgaggacg acatcatcca gcaggacggc gtcttcgtgc agtactacgc gccgaagagg
     5101 tgattggccc cctatttctg accggctgaa cgggacgtcc gggccgaatc gtcctggagc
     5161 agctccgccg ggacacgatg gacgacggag cgcggggcct cagccgcagg tcttatacag
     5221 ccggatgttg cggaacgaga acgccggacc gcgcaagtcg acggcctcgc cgtccggggt
     5281 gaagaagagg cccggggcgt gctccgagag gcggaagtcc tggccgtctc cctggaagtg
     5341 gagccagccg ttcctcacgg agacgttgta gaacttctcg ccgaccccga accgcttcct
     5401 cacgtagctg ccgacgtaga cgctccattc cttcctgtcg gggcctttcg gctcgctcgc
     5461 gggcgacccc atcgtccaca cggtcccgtc gtacggcctg acgacggaga ccggcttccc
     5521 gccggtgtcg cagacgaacc ggtagaggta gcgcttcgtc gcgtactcgt acttcgagat
     5581 cggcgtgatg ggcatgaagg tgttgccgga ggcgtagccg aaggtgccgt ccttgacgag
     5641 gaaggtcatg tcgtcgttcg ttccgcagta ccgtcccgcg agctcgaccg gaagggaaga
     5701 gggatcggcc gtccggggag gggtgtggcg ggccagccag tcggaaggcc cctggtcctt
     5761 cctgccggtg agaagctcca cgaccttcag gaggaggtcc tcggcgaggg cgtcgttgac
     5821 gttgtcgtgg tcctgggcgt tggtcaggac gacgacgccg taccccaggt cgggcagcca
     5881 cttcatctgg caccggaatc cgaagccgcc gcctccatgc gcgagccaga gcacgtcgtc
     5941 gccgccgaga tggtaggcgc cgacccagag gcccatgccg tagccggcca cctggtcctt
     6001 catcgggaag ggaatcgtcc gcatctggcg gaggacgcgc tcgtcgatca gcgggcgtcc
     6061 gtcgaccctg ccgtcgttca tctggaacct gaggaacacg gccatgtcgc caatggtcga
     6121 gtacgcgccg ccgggagcga gcatggggtt ctcgagggga acgctctcga agctcctgtt
     6181 gttgccgact gcccggttgc cgtccttccg gatccggtcg atgtcccagg tcgtccgacg
     6241 catcccgagc gggtcgagga ccttcctctg gacgtactcg gggaagggcc ggtgcgtgat
     6301 cgactccagg atgtacgcgg ccagctcgac gccgaggttc gagtactccg cccgctcgcc
     6361 ggtccgaaag cgcagccagg tgcgggagat gctccggatg tggtcttcgt acgaaggggt
     6421 gtacgggtcg gcgttgtttc cgacgggcgc gtcgtgggtc aggccggagg tcatgctcag
     6481 gaggtgccgg atggtgatga ccgacatcgg gtctt
//
LOCUS       DAOTEZ010000232         6514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_8470_length_6514_cov_5.927388, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000232 DAOTEZ010000000
VERSION     DAOTEZ010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6514
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8470_length_6514_cov_5.927388"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="P5164_12515"
     CDS             <1..299
                     /locus_tag="P5164_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44757.1"
                     /translation="RASLEATWYPTRRLAVGAGLAYETRDASSGSARSLRPSSEETTR
                     TLGLAARWFATERAAVDLGISRSEAEAISPPGGSTYQRFDETTTRLVLGAAVRF"
     gene            303..2294
                     /locus_tag="P5164_12520"
     CDS             303..2294
                     /locus_tag="P5164_12520"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRY44758.1"
                     /translation="MGESPFLRDLVIVLAASLPVLFVFQKLRVPTIVAFLFTGILIGP
                     YALGLVGDPDQVRRIAELGVVLILFYVGLGFSLPRLKELGRTAVVSGALQMTLAVLAT
                     AGVLLLLGQDLRPAIFGGLLVAGSSTAVVLPVLSARDEIDAPFARQFLGTSLFQDLGV
                     IPLILLLPALAGGTQAGEAPPLLSVLGRVGLAAASVAVLVAAARFVVPPLLDRLVRVA
                     GRETFTAGVVVVILGMVAIAVEAGVSAAMGAFAAGIVVAESESMADVSATFAPFRDIL
                     TSLFFVSIGMLLDPGFVLSRPLLVLGVVVAVVLLKVVTAYPALRAARAVPRVAIRAAF
                     ALATVGEFSFVLALAGLGYGLLQEGPQQLFVAVSVLTMLSAPLLVAAGARLVGLFPER
                     LEGADAPNASVLRSGHVVVVGYGLNGSNVARALGEIGIPYVVLDAEADRVSRARAAGV
                     EVLLADATSPAALEIAGIARARALVVTVPDPAASRKIVRMARSRNSGVKTIVRTRYIR
                     EVDELRRAGADEVIPEEFETSIEIVARLLRSFHVPGNLVATQIRVLRDEAYRKLRDPQ
                     SRPETGRRLSALMAAGTSDLVLVLPEMAASGKTLGELDLESDHVAVPALLRDGAPLAP
                     PPRELPIEPGDTLLLVGAHEDLVRATRKLEGAGPADPDA"
     gene            complement(2326..2790)
                     /locus_tag="P5164_12525"
     CDS             complement(2326..2790)
                     /locus_tag="P5164_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HRY44759.1"
                     /translation="MPVRKLKEFLDQNGVRWVSIVHSPAYTAQEVAASAHVKGKELAK
                     TVMVKVDGKPVMVVLPASQRVDFQVLREVTGGQNVVLASEAEFRELFPDCEAGAMPPF
                     GNLYGMDVYVAPKLAEDEEIAFNAGSHTELMKLRYADFERLVKPKVAAFAQR"
     gene            complement(2984..4213)
                     /locus_tag="P5164_12530"
     CDS             complement(2984..4213)
                     /locus_tag="P5164_12530"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY44760.1"
                     /translation="MTPAALLLAAIALAGLAITSLPVALLHRLRRSKRPLSRGPRGRA
                     SAPSPRAASTPDRPRLSILKPLKGLDDGLEENLASFAALRGVDYEVVLSVADSEDPAL
                     AVVDRVRARHPEAPFVLVVGGAPEGRIGNPKVERLVAASRVARGDLFLVSDSNVRVAP
                     DAVAETVVLFDEPSVGCVSNLFVGAGARDLGAVVEILHLLTFVVPGNVLAAWHGIPCV
                     VGKSMALPRHVHDAIGGFAAFGDVLAEDQAIGLAVKAAGFRVLLSPVVVENVVERRTL
                     KRALERQVRWGKIRFAFSKALFAGEVLMNPLPLALLAPPLALAGGSPWAGPLASFALL
                     ALLVRLLQASLLGRLCGAPLPLAALLAVPLKDVLQVATQAVPFVSREVVWHGHRARIG
                     TGTTLLEAGPRVRLARA"
     gene            complement(4210..4968)
                     /locus_tag="P5164_12535"
     CDS             complement(4210..4968)
                     /locus_tag="P5164_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRY44761.1"
                     /translation="MLLGDFHIHTTWSDGRHPLPEVVDLFGRSGHDVIAITDHVVNAD
                     SLLGKAAHGFRLSVTRDGWPAYRAEIERESRRAWDTYRMLVLPGVELTRNGLTGRSSA
                     HALALGLDDFVSADGPVEEALARARDAGAVTVACHPNEQSEWFANTFYLWNRKDAVRE
                     LVHLWELACRWDLFPEVGRSGLAFLGNSDFHRAPHLWSWKTLLDCEKKPEAVLAALRR
                     GRGLGLTRLHAPAPSPAPGPEPCEQLPAFARASA"
     gene            complement(5158..6375)
                     /locus_tag="P5164_12540"
     CDS             complement(5158..6375)
                     /locus_tag="P5164_12540"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRY44762.1"
                     /translation="MALFGSRPAGDARLGEAVEECRKRLRADPADGAAALRLADLLSA
                     SDGRAEAVGILNRVGARLRKAGRLVEAIAVFKKVEALDPRAEVTSSYLSILELKKLLE
                     AEERGRPPAAPLPESSAADAAATDRRKKSARVHTLRKEVPLLRDVPAFLFELVLEKIR
                     LFSLAPGATLFAEGEPGGTLFFVATGEIDTFARGDAGAPVPLERHGTGDVLGVVPFLS
                     GVPHPESAVARTHADLLGLDRAALAPLVKKHREIADALSKLFSERVLDGALARSRLFG
                     LLPRRDREAVAHRMRPWTVKAGSVVLREGEPGGDAFLVRSGALRVTTRLGGRELALAL
                     LTPHELFGDVASLRHGPRTATVTAVTECELLRLPAADLRALLAARPELAPALEEIQLE
                     RFTRNAAKLAKGS"
BASE COUNT          828 a         2297 c         2503 g          886 t
ORIGIN      
        1 ggcgggcgtc gctcgaggcg acgtggtacc cgacgcggcg gctcgcggtg ggcgcgggcc
       61 tcgcgtacga gacgcgggac gcctcgtccg ggtcggcccg gtctctccgg ccctcgagcg
      121 aggagacgac gaggacgctc ggcctcgccg cacgctggtt cgcgaccgag agggcagccg
      181 tcgacctcgg catctcccgg tcggaggccg aggcgatctc gccgcccgga gggtcgacgt
      241 accagcggtt cgacgagacg acgacgcgcc tggtcctcgg ggcggcggtg aggttctagg
      301 cggtgggaga gagcccgttc ctccgcgacc tcgtcatcgt cctcgccgcg tcgcttccgg
      361 tcctcttcgt cttccagaag ctgcgcgtgc cgacgatcgt ggcgttcctc ttcaccggga
      421 tcctgatcgg gccctacgcg ctcggcctcg tcggcgaccc cgaccaggtc cggaggatcg
      481 ccgagctcgg cgtcgtcctg atcctcttct acgtcgggct cggcttctcg ctcccgcggt
      541 tgaaggagct ggggcggacc gcggtcgtga gcggcgccct ccagatgacg ctcgccgtcc
      601 tcgccaccgc gggggtcctc ctcctcctcg gccaggacct ccgaccggcg atcttcggcg
      661 gtctcctcgt cgccggctct tcgacggcgg tcgtcctccc ggtcctctcc gcgcgggacg
      721 agatcgacgc cccgttcgcc cggcagttcc tcgggacgtc gctcttccag gacctcggcg
      781 tcatcccgct catcctcctc cttccggctc tcgccggcgg gacgcaggcg ggcgaggcgc
      841 ctccgctcct ctccgtcctc ggccgggtcg gcctcgcggc cgcgagcgtc gcggtcctcg
      901 tcgccgcggc gcgcttcgtc gtcccgccgc tcctcgaccg gctcgtccgc gtcgccggcc
      961 gggagacgtt cacggccggc gtcgtcgtcg tgatcctcgg gatggtcgcg atcgccgtcg
     1021 aggcaggggt ctcggccgcg atgggggcgt tcgccgccgg gatcgtcgtc gccgagagcg
     1081 agtcgatggc cgacgtcagc gcgacgttcg cgccgttccg cgacatcctg acgagcctct
     1141 tcttcgtctc gatcgggatg ctcctcgacc cggggttcgt cctgtcgcgc ccgctcctcg
     1201 tcctcggtgt cgtcgtcgcc gtcgtcctgc tgaaggtcgt cacggcctac ccggcgctcc
     1261 gggcggcgcg cgccgttccg cgcgtcgcga tccgggccgc cttcgcgctc gcgacggtcg
     1321 gggagttctc cttcgtcctc gcgctcgcgg ggctcggcta cgggctcctc caggagggtc
     1381 cgcagcagct cttcgtcgcg gtttcggtcc tgacgatgct ctcggcgccg ctcctcgtcg
     1441 ccgcgggggc gcgcctcgtg ggcctcttcc cggagcgcct cgagggggcc gatgcgccga
     1501 acgcctcggt cctccggtcg gggcacgtcg tcgtcgtcgg ctacggcctg aacgggagca
     1561 acgtggcgcg ggcgctcggc gagatcggga tcccctacgt cgtcctcgac gccgaggccg
     1621 accgcgtctc ccgcgcccgg gcggcggggg tggaggtcct cctcgccgac gcgacctcac
     1681 cggccgctct cgagatcgcg gggatcgcgc gggcccgggc gctcgtcgtg acggtccccg
     1741 acccggccgc gagccggaag atcgtccgga tggcccgatc ccggaacagc ggggtgaaga
     1801 cgatcgtcag gacgcgctac atccgcgagg tcgacgagct gcggcgcgcc ggcgccgacg
     1861 aggtgatccc ggaagaattc gagacctcga tcgagatcgt cgcgcggctc ctgcgctcgt
     1921 tccacgttcc ggggaacctc gtcgcgacgc agatccgcgt cctccgggac gaggcgtacc
     1981 ggaagctgcg cgacccgcag tctcggcccg agacgggacg ccgcctctcg gcgctgatgg
     2041 ccgcggggac ctccgacctc gtcctcgtcc tccccgagat ggcggcgtcc gggaagacgc
     2101 tcggcgagct ggacctggaa tcggaccacg tggcggtccc ggccctcctc cgggacggcg
     2161 ccccgctggc gccgcctccc cgggagctgc cgatcgagcc gggcgacacg ctcctcctcg
     2221 tgggcgcgca cgaggacctc gtccgcgcca cgaggaagct ggagggggcc ggcccagccg
     2281 atccggacgc ctgatcggtc cgccgagtgg acgggcggac cggccctacc gctgcgcgaa
     2341 cgccgccacc ttcggcttca cgaggcgttc gaagtcggcg tagcggagct tcatcagctc
     2401 cgtgtgcgat ccggcgttga acgcgatctc ctcgtcctcg gcgagcttcg gcgccacgta
     2461 gacgtccatc ccgtagaggt tcccgaacgg gggcatcgcg cccgcctcgc agtcggggaa
     2521 gagctcgcgg aactccgcct cgctcgcgag gacgacgttc tgtccgccgg tcacctcccg
     2581 gaggacctgg aagtcgacgc gctgcgacgc cgggaggacg accatcaccg gcttgccgtc
     2641 gaccttcacc atgacggtct tggcgagctc cttccccttg acgtgcgcgg aggcggcgac
     2701 ctcctgggcc gtgtaggccg gggagtggac gatcgatacc cagcggacgc cgttctggtc
     2761 gagaaactcc ttcagcttcc ggacgggcat gggaccctcc tttccggccg ggcggggcgc
     2821 gccccgtccg gcccgttcac gggcgctcgg tggggctcgg tgtggtgcgg cgcgctcatg
     2881 ccgtcacctc cgaagaaggg cgggtgcgag gggaattcta tgcccgcgcg gccgagggga
     2941 atcgggagcc gcggcggccc gtgacgcggg gcggacgacc ggctcaggct cgggcgaggc
     3001 ggacgcgggg cccggcttcg agcagagtcg tccccgttcc gattcgcgcc ctgtggccgt
     3061 gccagacgac ctcccgcgag acgaagggga ccgcctgcgt cgcgacctgg aggacgtcct
     3121 tcagcggcac ggcgaggagc gcggcgaggg ggagcggggc gccgcagagc cggccgagga
     3181 ggctcgcctg caggagtcgg acgaggagcg cgaggagggc gaaggaggcg agcggcccgg
     3241 cccagggaga gcctcccgcg agcgcgaggg gcggggcgag gagggcgagc ggaagggggt
     3301 tcatcaggac ctctcccgcg aagagcgcct tcgagaacgc gaaacggatc ttcccccagc
     3361 ggacctggcg ctcgagcgcg cgcttcaggg tgcggcgctc gacgacgttc tcgacgacga
     3421 cgggcgagag gaggacgcgg aagccggccg ccttcaccgc gaggccgatc gcctggtcct
     3481 ccgcgaggac gtccccgaac gcggcgaatc cgccgatcgc gtcgtggacg tgccgcggga
     3541 gcgccatcga cttgccgacg acgcagggga tgccgtgcca ggccgcgagg acgttcccgg
     3601 gaacgacgaa cgtcaggagg tggaggatct ccacgacggc gccgaggtcg cgcgctcctg
     3661 ccccgacgaa gaggttcgag acgcagccga cggacggctc gtcgaagagg acgacggttt
     3721 cggcgacggc gtcgggggcg acgcggacgt tcgagtcgga gacgaggaag aggtccccgc
     3781 gcgcgacgcg cgaggcggcg acgaggcgct ccaccttcgg gttcccgatc ctcccctccg
     3841 gagcgccgcc cacgacgagg acgaacgggg cctccggatg ccgtgcccgg acgcggtcga
     3901 cgacggcgag cgcggggtct tcggaatcgg cgacggagag gacgacctcg tagtcgactc
     3961 cccgaagagc ggcgaacgac gcgaggttct cttcgaggcc gtcatcgagc cccttcagcg
     4021 gcttgaggat ggagagccgc ggacggtcgg gcgtggaagc ggcccgcggg gaaggagcgg
     4081 aggcccgccc tcgcgggccg cgggagagag ggcgcttgct ccggcggagg cggtggagga
     4141 gcgccaccgg cagcgaggtg atcgcgaggc cggcgagggc gatcgccgcg aggaggaggg
     4201 cggcgggcgt cacgcggagg ccctcgcgaa ggcggggagc tgctcgcacg gctccggccc
     4261 gggggccggc gagggagccg gggcgtggag ccgcgtgagg ccgaggccgc gccccctgcg
     4321 gagcgcggcg aggaccgcct cgggcttctt ctcgcagtcg aggagcgtct tccacgacca
     4381 gaggtgaggc gcgcggtgaa agtcgctgtt gccgaggaac gcgaggcccg atcggcccac
     4441 ctcggggaag aggtcccagc ggcaggcgag ctcccagagg tggacgagct ccctcaccgc
     4501 gtccttccgg ttccagaggt agaaggtgtt cgcgaaccac tcggactgct cgttcgggtg
     4561 gcaggcgacc gtgacggcgc cggcgtcgcg ggcgcgcgcg agcgcctcct cgacgggccc
     4621 gtcggcggag acgaagtcgt cgaggccgag ggcgagcgcg tgggccgagc tccgcccggt
     4681 gaggccgttc cgcgtcagct cgacgccggg gaggacgagc atccggtagg tgtcccaggc
     4741 acgccggctc tcccgttcga tctcggcgcg gtacgcgggc catccgtcgc gcgtgacgga
     4801 gaggcggaag ccgtgcgccg ccttcccgag gagagagtcg gcgttcacga cgtggtcggt
     4861 gatcgcgatg acgtcgtgcc cggagcgccc gaagaggtcg acgacctccg ggagagggtg
     4921 ccggccgtcc gaccaggtcg tgtgaatgtg gaagtctccg aggagcatcc cgatcccccc
     4981 tttctcgcgc cccgaagatc ggcgcggcgc gggaggacgg ggtgaggaga cgatgaggac
     5041 cgggtaaagg tcggccgtgg ggtgccggtt cgggatcgcc gaggttccgg gccgaagggc
     5101 gatccacgag gcgggtacgg cggggcacgg tcgccgaccc gtcctcgccc gtcgccctca
     5161 gctccccttc gccagcttcg cggcgttcct cgtgaagcgc tcgagctgga tctcctcgag
     5221 ggcgggagcg agctccgggc gcgccgcgag gagggcgcgc aggtccgccg cggggaggcg
     5281 caggagctcg cactccgtca ccgccgtcac ggtggcggtg cgaggcccgt ggcggaggct
     5341 cgcgacgtcg ccgaagagct cgtggggcgt gaggagcgcg agggcgagct cgcgtccgcc
     5401 gaggcgcgtc gtgactcgga gcgcgccgct tcggacgagg aaggcgtcgc cgccgggctc
     5461 cccctcgcgg aggacgacgg atcccgcctt caccgtccac gggcgcatgc ggtgggcgac
     5521 ggcctcccgg tcgcgccgcg gcagaaggcc gaagaggcgc gagcgggcga gcgcgccgtc
     5581 gaggacccgt tcggagaaga gcttcgagag ggcgtcggcg atctcgcggt gcttcttcac
     5641 gaggggagcg agcgcggccc ggtcgaggcc gaggaggtcg gcgtgcgtgc gggccacggc
     5701 gctctccggg tgcgggacgc cgctcaggaa cgggacgacg ccgaggacgt cgccggtgcc
     5761 atgccgctcg agagggaccg gcgccccggc gtcgccgcgg gcgaacgtgt cgatctcccc
     5821 ggtcgccacg aagaagagcg tgccgccggg ctccccttcg gcgaagagcg tggccccggg
     5881 cgcgagcgag aagaggcgga tcttctcgag gacgagctcg aagaggaacg cggggacgtc
     5941 gcggaggagc gggacctcct tgcgcagggt atggacccgc gcggacttct tccgccggtc
     6001 ggtcgcggcc gcgtcggcgg ccgagctctc cgggagaggc gccgcaggcg gccggccgcg
     6061 ttcttccgcc tccaggagct tcttcagctc gaggatcgag aggtagctcg aggtgacctc
     6121 ggcccgcggg tcgagcgcct cgaccttctt gaagacggcg atcgcctcga cgaggcggcc
     6181 cgccttccgg aggcgcgctc cgacgcggtt gaggatcccg accgcctccg cacgcccgtc
     6241 cgaggcggag agaagatcgg cgagccggag ggccgcggcg ccgtcggcgg ggtcggcgcg
     6301 gagccgcttc cggcactcct ccacggcctc cccgaggcgc gcgtcacccg ccggccggct
     6361 cccgaacagc gccacggggg aatcgtatcc cgggcgcagc cgggaccggt gcgccctcgc
     6421 acgttccgcc gcggacgggg gcgcgcctat catcgggatc gttgctcccg cacctcctcc
     6481 tcgcgccgcc ggccgctgcc ggtctcgcgg agcc
//
LOCUS       DAOTEZ010000233         6505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_8489_length_6505_cov_6.779845, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000233 DAOTEZ010000000
VERSION     DAOTEZ010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8489_length_6505_cov_6.779845"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="P5164_12545"
     CDS             <1..820
                     /locus_tag="P5164_12545"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRY44763.1"
                     /translation="EWGFEVMIDPTKSPSVTAGADGKILQDFAISYSGLPGHDLQLGQ
                     KKVWLMEEGNRSSSALDFAERSVVARQFSDKRELGLFWKADWSPMVTTGVSVTQGTTL
                     NNNDNNDALNFNARLDVKPTKGLVVGASGGYSAGEGAAYLGRERYAAHVRWEGTEELP
                     LGFRFEYAEGRDEQASGGVVTKLKRNGWYGTVLYTFAKKFQLAARYEEFDRNKDSATD
                     DKLTILTGGFHYFIKANNANLKLNVESIEDDRRSVNGVLDEKYLQAVVAAQISF"
     gene            complement(817..4158)
                     /locus_tag="P5164_12550"
     CDS             complement(817..4158)
                     /locus_tag="P5164_12550"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY44764.1"
                     /translation="MGDETRSSAAPPPGAPGSGRTAAAGLICALMSMAFGAAALVGWA
                     TGRKVLTAFDLASIPMAPSTALLFVVLGGVAAVAARPGRGTRARSASAVLASLAALSS
                     AVLLVASLRGAYWRIEHIGLRIEGRVSGWPLGHISPVAAATLLLVSVALLALQIPPGR
                     GRMRGWLAATGGLVALSCGVVFFLAYVMGEPLLYRGAVLPPSAPTSTALVTLGLAVVL
                     AARVREPRPVDEVRLPRSAEATLAGLFLLFAAGIVLWGATWFREQERRLREQVDLQVS
                     TIAELKATGLARWRTERLGDGRVLQESQAFRRLADGLLAGTLPSDDEAVLGQWLGSVR
                     ESYGYASVELLDAGGARRLSIPTDAPPIVGDDGRHFAELLRSGEARLVDLHTHEPRGM
                     LRMAVVVPVVLEGRPRGAVLLGIDVRSHLEAFLSDWPVPSQTASTLLVRRDGDAVLVA
                     RAADAAVKEPRIERVPRMQVEPMLERAAAGEEVVGLFPRPDGSQVVAAARRVAGSPWK
                     LVACVDVEEVYGPLRERLWLTVLAMAGLLAAVAAGVVAVWRRQLGQAERARRLAEREG
                     ASLREVIERSLNEIFVVDPATFRFLFANRGAARNLGYSEEELRGMTPMDLVPGMTPDD
                     LGAMLRATDARPGEVHRVETTHRRKGGSLYPIEVHLQRVSAPTGDVYLAVINDITERK
                     AAEGKIRHLNRVYAVLSDVNQAIVRERDVPSLAAEACRIAVSVGGFREAWLLLAEGEG
                     VRCVGRAPAGGADDAAGAAAWAETLARESIRSGRSAAAPPGPERGRAVSLPLVVEGDA
                     IGAFVLAAGTEDPLDAEEMRLLEEMALDVSFALESIRGEAARREAEAEVRRLNADLER
                     RVEERTAELASAVRELETFAYSVSHDLKAPLRAIDGYSHLLVEEHGGRLDGKARDYLE
                     RVRSGARRMAELIDALLAYSRVERRALLPETIPLGESVRLALTHAQEEVASTGASVSV
                     AVDGLVVRADREGLAIVLRNLLGNALKFRKGAEAPRIEIGGESSGGVCRFWVRDHGIG
                     FDEVYAEKIFENFQRLHRQEEFPGTGIGLALVRKAVERMGGSIRAESEPGHGATFFVE
                     LPGAASGKTPGAGAPGAERTSGSGG"
     gene            complement(4277..5095)
                     /locus_tag="P5164_12555"
     CDS             complement(4277..5095)
                     /locus_tag="P5164_12555"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY44765.1"
                     /translation="MTEADGKGPETILDVDFGRLYRQHYAASGGREKPPEAWDVRASD
                     LSRELEGSPYVEELLRLVDLTGCRTLLDVGCGPGTIALALAPRLDRVWGLDYSRGMLD
                     AMTRHAAARGLTNVEPILRSWEEPWDVVPACDLVLASRSTLVRDMEDALARLDAKAKR
                     RVCLTSLAGGRFVRADLLALLGRERPAYPDYIYVVNLLHRMGRHPRLDFIRAGRRPDE
                     KPGFEQLARRVEFSLGALTPEERARLEAWYREDPARAGTGREPSCWAFVSWEKE"
     gene            complement(5092..5637)
                     /locus_tag="P5164_12560"
     CDS             complement(5092..5637)
                     /locus_tag="P5164_12560"
                     /inference="COORDINATES: protein motif:HMM:NF022115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HRY44766.1"
                     /translation="MSTSPPAPEELVARWGSLPAGSLALLTGGRGAGKTRWCEALARA
                     ARGAGLAVRGVLSPAVLADGEKRALDLLDLATGERRFLAERARPDLPGTEGLGWRFDL
                     EALAAGNAALERVGACDLLIVDELGPLELGRGSGFTAAFGLLDARRYGLAIVVVRPAL
                     VAALQVRRPGSASLWDLGGAR"
     gene            complement(5634..6398)
                     /locus_tag="P5164_12565"
     CDS             complement(5634..6398)
                     /locus_tag="P5164_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY44767.1"
                     /translation="MRRLEARGLSFGYDGAPVGEGVDLDLAGGEIVCLLGPNGGGKTT
                     LFRTLLGVLPPIAGEVRAADRPLARLSRRERARLLGWVPQAHAGLFAFTVEEVVLMGR
                     TARRGRFAAPSRRDHDVVREALERLGIGRLAGRVYTQISGGERQLALVARALAQEAGV
                     LILDEPTASLDFGNQLRVLGEIRRLRDDGIGILMSTHQPEHALEVADRIALLEAGRIV
                     AEGAPRETATPERLARLYRADLAAVAARLAPSESRR"
     gene            complement(6395..>6505)
                     /locus_tag="P5164_12570"
     CDS             complement(6395..>6505)
                     /locus_tag="P5164_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRY44768.1"
                     /translation="TLARSAAAIELPLGILTAFVGAPAFLWLLARGGRFS"
BASE COUNT          916 a         2447 c         2357 g          785 t
ORIGIN      
        1 ggagtggggc ttcgaggtca tgatcgaccc gacgaagagc ccgagcgtca ccgccggcgc
       61 cgacgggaag atcctccagg acttcgccat ctcctactcc ggcctcccgg gccacgacct
      121 ccagctgggg cagaagaagg tctggctcat ggaggaaggg aaccgctcct cctcggccct
      181 cgacttcgcc gagcgctctg tcgtcgcccg ccagttctcc gacaagcgcg agctcggcct
      241 cttctggaag gccgactgga gccccatggt gacgaccggc gtgtcggtca cccaggggac
      301 gaccctcaac aacaacgaca acaacgacgc cctcaacttc aacgcgcgcc tcgacgtgaa
      361 gccgacgaag gggctcgtcg tcggcgcctc gggcggctac agcgccggcg agggcgccgc
      421 ctacctcggc cgcgagcgct acgccgcgca cgtccgctgg gaggggaccg aagagctccc
      481 cctcggcttc cgcttcgagt acgccgaggg gcgagacgag caggcctccg gcggcgtcgt
      541 gacgaagctg aagcggaacg gctggtacgg aaccgtcctc tacaccttcg cgaagaagtt
      601 ccagctcgcc gcccggtacg aggagttcga ccggaacaag gactcggcca ccgacgacaa
      661 gctcacgatc ctcaccggcg gattccacta cttcatcaag gcaaacaacg cgaacctgaa
      721 gctgaacgtc gagtcgatcg aggacgaccg ccgcagcgtg aacggcgtcc tcgacgagaa
      781 gtacctccag gccgtcgtcg cggcccagat ctccttctag cccccgctcc cggaggtcct
      841 ctcggccccc ggcgctccgg cgccgggggt ctttccgctc gcggcccccg ggagctcgac
      901 gaagaacgtc gcgccgtgcc cgggctcgct ctcggcccgg atcgagccgc ccatccgctc
      961 cacggccttc ctcacgaggg cgagcccgat gccggtcccc gggaactcct cctgccggtg
     1021 gagccgctgg aagttctcga agatcttctc ggcgtacacc tcgtcgaacc cgattccgtg
     1081 atcgcggacc cagaagcggc agacgcctcc cgaggactcg ccgccgatct cgattcgcgg
     1141 cgcctccgcg cccttccgga acttcagcgc gttgccgagg aggttgcgca ggacgatcgc
     1201 gagcccttcc cgatcggcgc gcacgacgag gccgtcgacg gcgacgctca cggaggcgcc
     1261 ggtggaggcg acctcctcct gcgcgtgcgt cagcgccagg cggacgctct cgccgagcgg
     1321 aatcgtctcg ggaaggaggg cccgccgctc gacccgcgag taggcgagga gggcgtcgat
     1381 cagctcggcc atccgccgcg ccccgctccg gaccctctcg aggtagtcgc gggccttccc
     1441 gtcgagccgg cctccgtgct cctcgacgag gaggtggctg tagccgtcga tcgcccggag
     1501 cggcgccttc aggtcgtgag agacggagta ggcgaacgtc tcgagctcgc gcaccgcgct
     1561 cgcgagctcg gccgtgcgct cctcgacgcg ccgctcgagg tcggcgttca ggcgccggac
     1621 ctcggcctcc gcctccctcc gggcggcttc tccccggatc gactcgagcg cgaacgagac
     1681 gtcgagggcc atctcctcga ggagcctcat ctcctcggcg tcgagagggt cttccgtgcc
     1741 cgcggcgagg acgaaggctc cgatggcgtc cccttcgacg acgagcggca gcgaaaccgc
     1801 ccggccgcgc tcgggccccg gcggcgcggc cgcagagcga ccgctccgga tcgactcccg
     1861 ggcgagcgtc tcggcccagg ccgccgcacc cgcggcgtcg tccgcgcccc cggccggagc
     1921 ccggcccacg catcggacgc cctcgccctc cgcgagcagg agccacgcct ctcggaatcc
     1981 cccgacggag accgcgatcc ggcaggcctc ggcggcaagg gacgggacgt cccgctcgcg
     2041 gacgatcgcc tggttcacgt cgctgaggac cgcgtagacc cgattcaggt gccggatctt
     2101 cccctccgca gccttacgct ccgtgatgtc gttgatgacc gcgaggtaga cgtcgcccgt
     2161 gggggcgctc acccgctgga ggtggacctc gatcgggtag agactcccgc cctttcggcg
     2221 gtgggtggtc tcgaccctgt ggacctcgcc cggccgcgca tcggtcgctc ggagcatcgc
     2281 gccgaggtcg tcgggcgtca tccccgggac gaggtccatc ggggtcatcc cgcgcagctc
     2341 ctcctccgag tagccgaggt tccgtgccgc cccccggttc gcgaagagga agcggaaggt
     2401 ggccgggtcg acgacgaaga tctcgttcag gctccgctcg atgacctcac ggagcgaggc
     2461 gccttcccgt tccgcgagcc ttcgcgcgcg ctcggcctgt cccagctggc ggcgccagac
     2521 cgccaccacg cccgcggcca ccgccgcgag cagccccgcc atcgcgagga ccgtgagcca
     2581 gagccgttcg cgcagggggc cgtagacctc ctcgacgtcg acgcacgcga cgagcttcca
     2641 gggcgagccg gccacgcgtc gggccgcggc gacgacctgg ctcccgtcgg ggcggggaaa
     2701 gagcccgacc acctcctcgc cggcggccgc gcgttcgagc atcggctcga cctgcatcct
     2761 cgggacccgc tcgatcctcg gctccttcac ggccgcgtct gcggctcgcg ccacgagcac
     2821 cgcgtcgccg tcccttcgga cgaggagcgt cgacgccgtc tgcgagggga ccggccagtc
     2881 ggagaggaac gcctcgaggt gggaccgcac gtcgatcccg aggagcacgg cgccccgcgg
     2941 gcgcccctcg aggacgaccg ggacgacgac cgccatccgg agcatgcccc ttggctcgtg
     3001 cgtgtgaagg tcgacgagac gcgcttcgcc cgaccggagg agctcggcga agtgccggcc
     3061 gtcgtccccc acgatcggtg gggcgtcggt cgggatcgac agacggcgcg ccccgcccgc
     3121 atcgagaagc tcgacgctcg cgtagccgta gctctcccgg acgctgccga gccactgccc
     3181 gaggaccgcc tcgtcgtcgc tcgggagcgt cccggcgagg agaccgtcgg cgaggcggcg
     3241 gaaggcctgg ctttcctgga ggacccgtcc gtcgccgagc cgctcggtcc gccagcgggc
     3301 gaggccggtc gccttgagct ccgcgatcgt cgagacctgc aggtcgacct gctcccgcag
     3361 gcgccgctcc tgctcgcgga accaggtcgc cccccagagg acgattcccg cggcgaacag
     3421 caggaagagc cccgccagcg tcgcctccgc gctccggggc aggcgcacct cgtcgaccgg
     3481 ccgcggctcc cggacccgcg cggcgaggac gacggcgagg ccgagcgtca cgagcgccgt
     3541 gctcgtcggg gcggacggcg gcaggacggc gcctcggtag agcagcggct ctcccatcac
     3601 gtaggcgagg aagaagacga cgccgcacga gagcgccacg aggccgccgg tcgcggccag
     3661 ccagcctcgc atccggcccc ggcccggagg gatctggagc gcgaggagcg cgacgctcac
     3721 gaggaggagc gtggccgccg cgacgggcga aatgtgcccg agcggccagc cggagacgcg
     3781 cccctcgatc cgaaggccga tgtgctcgat ccgccagtac gctccgcgga ggctcgcgac
     3841 gagcaggacg gccgagctga gcgccgcgag agaggcgaga acggcgctcg cgctccgcgc
     3901 cctcgttcct cgtcccggtc gtgccgccac cgcggcgacg cccccgagga ccacgaagag
     3961 gagcgccgtg ctcggcgcca tcgggatgct cgcgagatcg aacgcggtca gcaccttccg
     4021 gccggtcgcc catccgacga gagccgccgc gccgaacgcc atggacatca gggcgcagat
     4081 caggccggcg gccgccgtcc ggccggagcc gggggcgccc ggggggggcg cagccgaaga
     4141 gcgcgtctcg tctcccactg tccagcctcc ctccagtatg ccccctccgc gccgagccag
     4201 cccttcgggg ccgggccggc tgctcccgcc ccgagccccg acgttccggg tcgccgaccg
     4261 gatccccggt ccctcgctac tccttctccc aggagacgaa cgcccagcag gacggttcgc
     4321 ggccggtccc ggcgcgggcg gggtcctccc ggtaccaggc ctcgagccgc gcgcgctcct
     4381 cgggggtgag ggcgccgagg gagaactcga cgcgccgggc gagctgctcg aaacccggct
     4441 tctcgtcggg ccggcgcccg gcccggatga agtcgaggcg cgggtggcgg cccatccggt
     4501 ggaggaggtt cacgacgtag atgtaatcgg gataggcggg ccgctcgcgg ccgaggagcg
     4561 cgaggaggtc ggcgcggacg aaacgccctc cggcgaggct cgtgaggcag acgcgccgct
     4621 tcgccttcgc gtcgagacgg gcgagggcgt cctccatgtc gcggacgagc gtcgatcgcg
     4681 aggcgaggac gaggtcgcac gccgggacga cgtcccacgg ctcttcccag gagcggagga
     4741 tcggctcgac gttcgtaagg ccccgtgcgg cggcgtggcg cgtcatcgcg tcgagcatcc
     4801 cgcggctgta gtcgaggccc cagacgcgat ccaggcgcgg ggcgagggcg agggcgatcg
     4861 tcccggggcc gcacccgacg tcgaggagcg tgcggcagcc cgtcaggtcg acgagccgga
     4921 ggagctcctc gacgtacggg cttccttcga gctcgcggct caggtcggaa gcccgcacgt
     4981 cccacgcctc gggcggcttc tcgcgcccgc cgctcgcggc gtagtgctgt cggtagagac
     5041 gtccgaagtc gacgtcgagg atcgtctcgg ggcccttccc gtcggcttcg gtcatcgggc
     5101 ccccccgagg tcccagagcg acgcggagcc gggccggcgc acctgcaggg cggcgacgag
     5161 cgccggccgg acgacgacga tcgcgaggcc gtagcggcgg gcgtcgagga ggccgaacgc
     5221 cgcggtgaag ccgctcccgc ggccgagctc cagcgggccg agctcgtcga cgatcaggag
     5281 gtcgcacgcc cccaccctct cgagcgcggc gttgcccgcg gcgagggcct cgaggtcgaa
     5341 gcgccagccg aggccctcgg tcccggggag gtcggggcgg gcccgctcgg ccaggaaacg
     5401 gcgttcgccc gtggcgaggt cgaggaggtc gagggcgcgc ttctcgccgt ctgcgaggac
     5461 ggccggcgag aggacgccgc ggacggcgag ccccgcgccc cgtgccgcgc gcgccagggc
     5521 ctcgcaccag cgcgtcttcc ccgcgccccg cccgccggtc aggagcgcga gagagcccgc
     5581 cggaagcgag ccccaccgcg cgacgagctc ctccggcgcc ggcggagacg tgctcaccgc
     5641 ctcgactccg agggcgcgag acgcgcggcg acggccgcga ggtcggcccg gtagaggcgc
     5701 gccaggcgct cgggcgtcgc ggtctctcgc ggcgctccct ccgcgacgat ccgccccgcc
     5761 tcgaggagcg cgatccggtc ggcgacctcc agcgcgtgct cgggctggtg ggtcgacatc
     5821 aggatgccga tgccgtcgtc gcgaaggcgg cggatctccc cgaggacccg cagctggttc
     5881 ccgaagtcga ggctcgccgt cggctcgtcg aggatcagga cgccggcctc ctgcgcgagc
     5941 gcccgggcga cgagagcgag ctggcgctcg ccgccggaga tctgcgtgta gacgcggccg
     6001 gccagccggc cgatcccgag gcgctcgagc gcctcgcgga cgacgtcgtg gtcgcgccgc
     6061 gacggggcgg cgaagcggcc gcgccgggcc gtccggccca tcagcacgac ctcctccacg
     6121 gtgaaggcga agagtccggc gtgcgcctgc gggacccagc cgaggaggcg cgccctttcg
     6181 cgccgcgaca agcgggcgag cggccggtcc gcggcgcgca cctcgccggc gatcggcggc
     6241 aggacgccga ggagcgtccg gaagagcgtc gtcttccctc ccccgttcgg gccgaggagg
     6301 cagacgatct ccccgcccgc gaggtcgagg tcgacccctt cgccgacggg agccccgtcg
     6361 tagccgaagc tcaggccgcg cgcctcgagg cgcctcacga gaagcggcct ccccgcgcga
     6421 ggagccagag gaaggcgggc gcgccgacga acgccgtgag gatgccgagc gggagctcga
     6481 tcgcggcggc gctgcgggcg agcgt
//
LOCUS       DAOTEZ010000234         6500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_8504_length_6500_cov_3.513887, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000234 DAOTEZ010000000
VERSION     DAOTEZ010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6500
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8504_length_6500_cov_3.513887"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="P5164_12575"
     CDS             <1..218
                     /locus_tag="P5164_12575"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRY44769.1"
                     /translation="RADYVEIYNLDEGRGRPRAVDDDFIADVKTHIREAADGLRSGAM
                     PPRPEQKRCAGCDYRRLCTAGCAAGAS"
     gene            264..575
                     /locus_tag="P5164_12580"
     CDS             264..575
                     /locus_tag="P5164_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002750887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRY44770.1"
                     /translation="MSPAVTRRQEVLERIRSERERFRGLGVRRLGLFGSFARDEATGE
                     SDVDLLVEFEPGAKSYRRFMSAVFLLEELLGREVELVTPEGLSPYLRDEIARSVVDVG
                     P"
     gene            562..888
                     /locus_tag="P5164_12585"
     CDS             562..888
                     /locus_tag="P5164_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRY44771.1"
                     /translation="MSDRDLVRHIFDEVVFLRTAASATTLEAFLGDEVLKRAFARSLE
                     IVGEASKKLTPEFREAHPSIEWRLMAGTRDKLIHDYFGVDYGLVWDIVVNELPGLETE
                     LRGLLE"
     gene            1026..3914
                     /locus_tag="P5164_12590"
     CDS             1026..3914
                     /locus_tag="P5164_12590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRY44772.1"
                     /translation="MKLKPAVLGVLGRDDLKAFVEMWEIDGVDKRSVEAMRAALSRSR
                     RADVAVLLDSIREDAVKEVCDLVGVDRTGRRSVLVERLLAAGEASGAAGDGADSELPA
                     KGASAAKGTRAAAKGGTRSSPAANGRKVTRYTYGDVVEPRTPETGHTALLPAEDLVVT
                     LPMDNGWSKAIEVGKLPEGVDRPVVLDMDPAADPVLFWAGKRSRREVPVLPLQRNEIV
                     SESRLAQIVERARKEALKEKEEGQSLLFADLERELREGEKDRRVDFYTHEEGWKNKLI
                     CGDSLHVMESLLHYEGLRGKVQMIYIDPPYGIKYDSNFQQRVDSTKNDEKDQGDDVLT
                     IKAFRDTWALGVHSYLSYLQERLYLCREMLAETGSIFVQIGEDLVHVVRALLDEVFGA
                     KSFLSQIAFSKTSGKSGGSLDSVYDILLWYAKDAGAVRSKVLYAQRPDYTLAEQYTLV
                     ELKDGTVRSATERELSGEERLPVGARRFMPDQLSSQGATEEGSKPYRFNGVVYELPPN
                     SHWKMRNPEGLDGLAAKGRIHPIGKRLRYKRYADDFPFAPRSNIWDDTVISGFASQKL
                     YAVHTSTKVVDRCILMTTDPGDLVFDPTCGSGTTAFCAERIGRRWITCDTSRVAVNVA
                     RQRLLSGTFEHYKTRNGTVSSGFRYRTVPHVTPKSLAYDLEPEQVELVDQPEVEKGVV
                     RVCGPFEVMTVGRYSVEDWKGYVVAETTAPYGAEPEPPKLENYIEVIARLYRKNAAVQ
                     GASGLVHAVAESNSERIAISVGPLSGRVTAKQVNDAVQDALSSGLLEVHVLGWAFEAN
                     VGEVKAQLEKRGKVRVELIMIRPDTLAEGLKITKPETLFSPLALPDVELKVTGGKEKK
                     AVVTLKGVAVFDRKSRATEYRRADSGYVSAWYLDEDYDGDCFVDGQMFFDFKKAPNLR
                     AALKADVDPEEYRLKLSSEPFPVRSHGRIAVKVVDVFGNESTVVREIA"
     gene            3915..>6500
                     /locus_tag="P5164_12595"
     CDS             3915..>6500
                     /locus_tag="P5164_12595"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRY44773.1"
                     /translation="MAYFVDRVVICDAYAPPDRHYEILPGGRSKLVPGRRPSKRYVVG
                     GREARGGIGTIVGPDPGLFDDEVEAANEVRNEFVNELREKVAAWRDGGYVSPAPALVT
                     RRLLEWWFERDEERKAVGKRLFFCQQEAIETLVYLYECEGRRRLPETGSLVRYALKLA
                     TGTGKTVVMALLVVWSTLHRRKVSGSPLSSNFLVLVPNLTVRSRVSGQPRGDGLDPGG
                     AQNLYDAFDLVPPEYREEFRPNVLVRNWQSIPLEARRDDWIPEESLEAGRFIPQAVLR
                     AMKRRAGQDPDNVVRREISGWRDLVVVNDEAHHVYGEKRARAGEEPEYIKWSRILERI
                     AKASRVGLVVDLSATPWYGSGSAKPEGTLFEWLASDFSVYDAFESGLVKVVRLPDPDE
                     AGRKYLDLWDDVRGAKTEHEYVMACKGAIASLYSSWRHDWDEWSSSFEFARGPSPVLL
                     CVTDNATRARWLFDHLTKDYALLKNPDDEDPREWKTIQIDSKVFDADKGREGVIREMV
                     DTVGKKGAPGEHVRAIVSVNMLSEGWDVKSVTHILGLRAFGSPLLTEQVVGRGLRRTT
                     YDVLNQPLEERPAESDETVDAFGIPFVGFPVEKRKRPQAGSWNRQKPVTIEPDPKKTG
                     NALRVPNVRAWAVGVTGTLADAVRPSDIPILTIDPRRTPPEVTVRPVVGGRPEAVLTL
                     ESFREEWPVLKTAFLLAQELFEATNPDSARDLNVGPTFEELLEVVQAYLASRVRVLPG
                     AAPEDLGIYYWRVQAKDALETAIRSAGRVGTRPVPIPGEPEWLDSGKLRKFQWTGLVA
                     EGKKAATNRVPCHTDLEREFAGFLDAAPDVLRYLKNERFGFSVTYYEGGRPRQYFPDF
                     VARVLE"
BASE COUNT         1076 a         2127 c         2346 g          951 t
ORIGIN      
        1 ggcgggccga ctacgtcgag atctacaacc tcgacgaggg gagggggcgg ccccgcgccg
       61 tggacgacga cttcatcgcc gacgtcaaga cgcacatccg cgaggcggcg gacggcctcc
      121 ggagcggcgc gatgccgccg cggccggagc agaagcgctg cgccggctgc gactaccgtc
      181 gcctctgcac ggcgggctgt gcggccgggg cgtcgtaaga ttcccggcgg atggccgggt
      241 ccgcgacccg gtcgccccga acgatgagcc ccgccgtaac gcgtcgacag gaggtcctcg
      301 agaggatccg ctccgagagg gagcggttcc gtggcctcgg cgtccgccgc ctcggcctct
      361 tcggctcctt cgcccgcgac gaggccacgg gggagagcga cgtcgacctg ctcgtcgagt
      421 tcgagccggg cgccaagagc taccggcgct tcatgtccgc cgtcttcctc ctcgaggagc
      481 tcctcggccg ggaggtcgag ctcgtcaccc cggagggcct cagcccctac ctgcgcgacg
      541 agatcgcccg gagcgtcgtc gatgtcggac cgtgacctcg tccggcacat cttcgacgag
      601 gtcgtcttcc tccggacggc cgcctcggcg acgacactcg aagccttcct cggcgatgag
      661 gtcctgaaac gggccttcgc ccggagcctc gagatcgtcg gcgaggcctc caagaagctg
      721 acgccggagt tccgcgaggc gcacccctcg atcgagtggc gcctcatggc gggcacccgc
      781 gacaagctga tccacgacta cttcggggtc gactacggcc tcgtgtggga catcgtcgtg
      841 aacgagctgc ccggtctcga gacggagctg cgcggcctgc tggagtaggc cggcgtcgag
      901 cgggaggtgc catccctcgc gcagtcggtg cagccgccga ttgtcgggcg gctccgagac
      961 acgcgcacca tgtaaccttg acgagctcgt cccgggccgt atcccgggcg cgcgatgggg
     1021 ggccgatgaa gctgaagcct gccgttctcg gcgttctcgg aagagacgac ctcaaggcgt
     1081 tcgtcgagat gtgggagatc gacggcgtcg acaagaggag cgtggaggcg atgcgcgcgg
     1141 cgctctcgcg cagccgcagg gccgacgtcg ccgtcctcct cgattccatc cgggaggacg
     1201 ccgtcaagga ggtctgtgac ctcgtcggcg tcgacagaac cgggcgaagg tcggtcctcg
     1261 tcgagcggct cctggcggcg ggcgaggcct cgggggccgc gggcgacggc gccgactccg
     1321 agctgccggc gaagggcgcc tcggcggcga agggaaccag ggccgccgcg aagggcggga
     1381 cgaggtcgtc gccggcggcg aacggacgaa aggtgacccg ctacacctac ggcgacgtcg
     1441 tcgagccgcg gacgcccgag acggggcaca ctgccctcct ccccgcggaa gacctcgtcg
     1501 tgacgctccc gatggacaac ggctggtcga aggcgatcga ggtcggcaag ctccccgaag
     1561 gggtcgatcg ccccgtcgtc ctcgacatgg acccggccgc cgacccggtc ctcttctggg
     1621 cggggaagcg gagccgccgc gaggtcccgg tcctcccgct ccagcggaac gagatcgtct
     1681 ccgagtcgcg cctcgcgcag atcgtggagc gggcgcggaa ggaggcgctg aaggaaaagg
     1741 aggaggggca gagcctcctc ttcgcggacc tcgagaggga gctgcgggaa ggcgagaagg
     1801 atcgccgcgt cgacttctac acccacgagg aggggtggaa gaacaagctc atctgcggcg
     1861 actcgctcca cgtcatggag tcgctcctcc actacgaggg gctccgcggc aaggtccaga
     1921 tgatctacat cgacccgccg tacggcatca agtacgacag caacttccag cagcgggtcg
     1981 actcgacgaa gaacgacgag aaggaccagg gcgacgacgt cctgacgatc aaggcgttcc
     2041 gggacacgtg ggccctcggc gtgcattcgt acctgtcgta cctgcaggag aggctctacc
     2101 tgtgccggga gatgctggcg gagacggggt cgatcttcgt gcagatcggg gaggacctcg
     2161 tccacgtggt gcgggcgctt ctcgacgagg ttttcggagc gaagtcgttc ctgagtcaga
     2221 ttgccttttc gaagacgtcg ggcaagagcg gcgggtctct cgatagcgtc tacgacatcc
     2281 tcctctggta cgcgaaggac gcgggcgcgg tccggtcgaa ggtgctttat gcacaacggc
     2341 ctgactacac gcttgcggag cagtacacgc tggtcgaact caaggacggg acggtgcggt
     2401 cagccactga gcgagagctt tcgggcgagg aaagactacc ggttggtgcc cgcaggttca
     2461 tgccagatca gctctcgtcg caaggtgcca cagaagaggg gtcgaagccg taccggttca
     2521 acggcgtagt gtacgaactg ccgccgaact cccattggaa gatgaggaat cctgaggggc
     2581 tcgacggcct cgcggccaag ggcagaatcc accccatcgg caagaggctt cgctacaagc
     2641 gctacgccga cgacttccca ttcgcgccga gatcgaacat ctgggacgac accgtaatca
     2701 gcggctttgc gtcccagaag ctctacgctg tccacacgtc aacgaaggtc gtggatcgct
     2761 gcatcctcat gacgaccgac cccggcgacc tcgtctttga ccccacctgt ggctctggca
     2821 cgacggcctt ctgcgccgaa cggatcggcc ggcgctggat cacctgcgac acgtcgcgcg
     2881 tggcggtgaa cgtcgcgagg caacgccttc tgagcgggac cttcgagcac tacaagaccc
     2941 gcaacggcac cgtctcgagc ggcttccgct acaggacggt cccccacgtg acgcccaaga
     3001 gcctcgccta cgacctcgag cccgagcagg tcgagctcgt cgaccagccc gaagtcgaga
     3061 agggcgtcgt ccgcgtctgc ggcccgttcg aggtgatgac ggtcggccgg tactccgtcg
     3121 aggactggaa ggggtacgtc gtcgcggaga cgaccgcgcc gtatggcgcg gagccggagc
     3181 cgccgaagct cgagaactac atcgaggtga tcgcgcggct ctaccggaag aacgcggccg
     3241 tccagggagc ctcgggcctc gtccacgctg tcgccgagtc gaactcggag cggatcgcga
     3301 tctcggtcgg gcctctctcg ggacgcgtga cggccaaaca ggtgaacgac gccgtccagg
     3361 acgcgctctc gtccggcctc ctcgaggtcc acgtcctcgg ctgggcgttc gaggcgaacg
     3421 tgggcgaggt gaaggcgcag ctcgagaagc gagggaaggt ccgggtggag ctgatcatga
     3481 tccggcccga cacgctcgcc gagggcctca agatcacgaa gccggagacg ctcttctcgc
     3541 cgctcgccct tccggacgtg gagttgaagg tgacgggcgg caaggagaag aaggccgtcg
     3601 tcacgctcaa gggggtcgcg gtcttcgacc ggaagtctcg ggcgaccgag taccggaggg
     3661 ccgactcggg ctacgtctcg gcctggtacc tcgacgagga ctacgacggc gactgcttcg
     3721 tcgacgggca gatgttcttc gacttcaaga aggcgccgaa cctccgggca gcgctgaagg
     3781 ccgacgtcga ccccgaggag taccggctga agctctcgtc cgagccgttc ccggtgcgaa
     3841 gccacgggcg gatcgccgtg aaggtggtgg acgtcttcgg gaacgagtcg acggtggtcc
     3901 gcgagatcgc gtagatggcg tacttcgtcg accgcgtcgt catctgcgac gcctacgcgc
     3961 cgccggaccg gcactacgag atcctccccg gcggccggtc gaagctcgtc ccgggccggc
     4021 ggccgtcgaa gcggtacgtc gtcggcggcc gggaggcccg gggcgggatc gggacgatcg
     4081 tcggcccgga tcccgggctc ttcgacgacg aggtggaagc cgccaacgag gtccggaacg
     4141 agttcgtcaa cgagctgcgg gagaaggtgg ccgcatggcg cgacggtggg tacgtctcgc
     4201 ccgctccggc gctcgtcacg cggcggctcc tcgagtggtg gttcgagcgc gacgaggagc
     4261 ggaaggcggt cggcaagcgg ctctttttct gccagcagga ggcgatcgag acgctcgtct
     4321 acctctacga gtgcgaggga cgtcggcgcc tccccgagac ggggagcctc gtccgctacg
     4381 cgctgaagct cgcgacgggg acggggaaga cggtcgtcat ggcgcttctc gtcgtctggt
     4441 cgacgctcca ccggaggaag gtgagcgggt cgcccctgtc gtcgaacttc ctcgtcctcg
     4501 tcccgaacct gacggtccgc tcgcgggtga gcggccagcc gcggggcgac ggcctcgacc
     4561 cgggcggcgc tcagaacctc tacgacgcct tcgacctcgt gccgccggag taccgggagg
     4621 agttccggcc gaacgtcctc gtgaggaact ggcagagcat cccgctcgag gcgcgtcggg
     4681 acgactggat ccccgaggag tcgctggagg cggggcggtt catcccgcag gcggtcctcc
     4741 gggcgatgaa gcggcgggcg gggcaggacc ctgacaacgt cgtccggcgg gagatctccg
     4801 gctggcgcga cctcgtcgtc gtcaacgacg aggcgcatca cgtctacggc gagaagcgcg
     4861 cgcgggccgg cgaggagccg gagtacatca agtggagccg catcctcgag cggatcgcga
     4921 aggcttcgcg cgtcgggctc gtcgtcgatc tctcggcgac gccgtggtac ggctcggggt
     4981 cggccaagcc cgaggggacg ctcttcgaat ggctcgcctc cgacttctcg gtctacgacg
     5041 cgttcgagtc ggggctcgtg aaggtcgtcc gtctccccga tccggacgag gccggccgga
     5101 aatacctcga cctctgggac gacgtccgcg gggcgaagac cgagcacgag tacgtcatgg
     5161 cctgcaaggg ggcgatcgcg agcctctact cgtcgtggag gcacgactgg gacgagtggt
     5221 cgtcgagctt cgagttcgcg cgcgggccga gcccggtcct cctctgcgtc accgacaacg
     5281 cgacgcgggc ccggtggctc ttcgaccacc tcacgaagga ctacgccctc ctgaagaacc
     5341 ccgacgacga agatccgcgc gagtggaaga cgatccagat cgactcgaag gtcttcgacg
     5401 ccgacaaggg gcgggagggc gtgatccgcg agatggtcga cacggtcggg aagaaggggg
     5461 cgcccgggga gcacgtccgc gccatcgtct cggtgaacat gctctcggag gggtgggacg
     5521 tgaagagcgt cacccacatc ctcggcctcc gggcgttcgg ctcgccgctc ctgacggagc
     5581 aggtcgtcgg gaggggcctc cggaggacga cctacgacgt cctgaatcag ccgctcgagg
     5641 agcgcccggc cgagtcggac gagacggtgg acgcctttgg catcccgttc gtcggcttcc
     5701 cggtggagaa gcgcaagcgc ccgcaggcgg ggagctggaa ccggcagaag ccggtgacga
     5761 tcgagcccga cccgaagaag acggggaacg ccctccgcgt gccgaacgtg cgggcctggg
     5821 ccgtcggcgt gacggggacg ctggccgatg cggtccgacc gagcgacatc ccgatcctca
     5881 cgattgaccc acgacggacg ccgccggagg tgaccgtccg cccggtcgtc ggcggccggc
     5941 cggaagcggt actgacgctg gagtcgttcc gggaggagtg gccggtcctg aagacggcct
     6001 tcctcctcgc gcaagagctg ttcgaggcga cgaaccccga ctcggcccgc gacctgaacg
     6061 tcggcccgac gttcgaggag cttctcgagg tggtccaggc gtacctcgcg tcgcgggtcc
     6121 gggtgctgcc cggggcggca ccggaagacc tcggcatcta ttactggcgg gtgcaggcga
     6181 aggacgccct cgagacggcg atccgctccg ccgggcgggt cgggacgcgg ccggtgccga
     6241 tccccgggga gcccgagtgg ctcgactcgg ggaagctccg gaagttccag tggacggggc
     6301 tcgtcgcgga ggggaagaag gccgcgacga accgggtgcc gtgccacacg gacctcgagc
     6361 gcgagttcgc cggcttcctc gacgccgcgc ccgacgttct ccggtacctg aagaacgagc
     6421 ggttcggctt ctcggtgacc tactacgaag ggggccgccc ccggcagtac ttcccggact
     6481 tcgtcgcgcg ggtgctggag
//
LOCUS       DAOTEZ010000235         6464 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_8566_length_6464_cov_7.247621, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000235 DAOTEZ010000000
VERSION     DAOTEZ010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6464
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8566_length_6464_cov_7.247621"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /gene="pstB"
                     /locus_tag="P5164_12600"
     CDS             complement(<1..790)
                     /gene="pstB"
                     /locus_tag="P5164_12600"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013109751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRY44774.1"
                     /translation="MEAQTMAPPVSATPLAVRTAPGPEAAPVAAPPLIELEKFSFWYG
                     PKQALFDIDLKVRERSVTAFIGPSGCGKSTLLRCLNRMNDLIDGVRQEGSLRVAGEDL
                     YAPDVDVVSVRKRLGMVFQKSNPFPKSIFENVVYGLRIQGERNKTVLRDACESALRGA
                     ALWDEAKDRLDESALALSGGQMQRLCIARALAVEPEVVLMDEPCSALDPIATARIEEL
                     IFALKERFTIVIVTHNMQQAARVSDETGFFMLGKLVEFDRTDVIF"
     gene            complement(802..2457)
                     /gene="pstA"
                     /locus_tag="P5164_12605"
     CDS             complement(802..2457)
                     /gene="pstA"
                     /locus_tag="P5164_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRY44775.1"
                     /translation="MSASEGRPSLARKSGIFRSGEPFIWVTGSGLAVAVLMVFGLLWL
                     VLANGLGFFWPADVHLFTLEDGGALAGRVAARERIPDPDAAPGTPDRWRIKVKQGNRD
                     LTGEDFVWVDEASIRSRATPADLVVLERREWGDFYGWVKEVRDGEASLASGREASWRK
                     VAELLPRAREVHEEALRLEKGPIGAVNFEKEKLRLERKKLELEGAAGGPKRDALDAEE
                     RALQAEYERLFTELQAVRARVKAILVLETAGGLTKELPLSQVVRADRVNGMGRLAKAG
                     RYAANVWGFVTEDPRESNTEGGVFPAIFGTVLMVLIMSVVVTPLGVLTAFYLREYAKQ
                     GPVVSAVRIAVNNLAGVPSIVYGVFGLGFFIYFVGGSIDRLFYPEALPAPTWGTGGIL
                     WASLTLSLLTVPVVIVSAEEGLAAIPRGMREASLALGATKFETMWRVVLPAVMPSILT
                     GLILAMARAAGEVAPLMITGVVKLAPSLPVDGFAPFFHLDRKFMHLGFHIYDVGFQSP
                     NVDAARPMVFTTTILLILIVVALNLTAIVIRGRLRRKLRSSGI"
     gene            complement(2454..4721)
                     /locus_tag="P5164_12610"
     CDS             complement(2454..4721)
                     /locus_tag="P5164_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY44776.1"
                     /translation="MSATIESSAKDGPPAPARAMGTSRPLGRRLLADRFAARFVTLSG
                     LAIIAAILAILFVIVAVVWPLFVPPSARTVPAGSLPAGAVPLALSVDEYREEAFVATQ
                     AGLVGLPVAGGELFTRELPALGDATVVKAGAPEPELIVLGLSDGRLYPVEVSTEVAYE
                     DGKRRVTPVVRPGEPVTVNADGMPVAVFKYVRGPDGPIALAATGPREITLVRVTERTS
                     LMGETTRETRSVVLPIEARGEIRTLALDVRGQDVFAGTSAGEIVRLDLRDEQSPKQEE
                     TVPASAAGREVTVLGFLNGDRTLVSGDADGRVASWQVVSSESGSSRRLTRMHDFRSHG
                     AAVTAFSPSRRDKGFVTADAAGSLAVHYATTGKTLLALSAGTAPLAAVVFAPKADGVL
                     AVAEDGRLHHFELRNPHPEVTWRSLFGKVWYEGYGRPDYVWQSTGGTDDFEAKLSLTP
                     LIYGTMKGTFYALVLAIPLALAAALYTAQFMHPRLRSFVKPVVEVMAALPSVVLGFIA
                     GLWLAPVVERFVPGVLVMPVVIAAFAALGAVVWGRLPARVRARIPAGWEALLLLPLVV
                     AGGAASLWVGAGFERYLLGADYRHFLLEALGLTYDQRNSLVVGLAMGFAVIPIIFTVA
                     EDSISAVPPGLSAGSLALGATRWQTALRTVLPTASPGIFSAIMIGFGRAVGETMIVLM
                     ATGNTPVMDPSIFNGFRALSANIAVELPEAPEGGTLFRVLFVAALLLFVMTFVVNTVA
                     ELVRLRLRKKYRTFE"
     gene            complement(4738..5703)
                     /locus_tag="P5164_12615"
     CDS             complement(4738..5703)
                     /locus_tag="P5164_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005032077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRY44777.1"
                     /translation="MKKTATLAAAALLAGTAAAQTIQVDPAIKPYKAVSGVSGNVSSI
                     GSDSLNNLMTLWAEKFNRMYPNVKIQIEGKGSSTAPPALISATAQLGPMSRAMKGSEI
                     DEFEKKFGYKPTQVRVAVDALAVFVNKDNPVACLTMPQVDAAFSKSRRAGYKENVKTW
                     GQLGLTGDWANRPVSLFGRNSASGTYGFFKEHVLKNGDYKDEVKEQPGSASVVQGVTV
                     DRFAMGYSGIGYATAGVRALPLAAKPGDKCHPATPEEAYSGNYPLARFLYVYVNKAPG
                     KPLDPIVGEFLKLVLSKDGQEVVVKDGYFPLPAKIEQEELAKLLK"
     gene            5907..>6464
                     /locus_tag="P5164_12620"
     CDS             5907..>6464
                     /locus_tag="P5164_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44778.1"
                     /translation="MDRTLRRLAGFALAGILLLAGAAPAQVNLLWYKEVAKDGRIYVF
                     NDPNEFKAWEGSGELGKSITKIGFGPNGETVVFDNDTALDLYVFKHGKEPEVRPYSAP
                     KPPKPVPPTTLKIGDGELKLGGLLQGWYVLDDSATGSGSSQLGNSTGVNTFRLRRAEI
                     KLSGKVTPEWGFEVMIDPTKSPSVTA"
BASE COUNT         1021 a         2279 c         2251 g          913 t
ORIGIN      
        1 tgaagatgac gtcggtccgg tcgaactcga cgagcttgcc gagcatgaag aagccggtct
       61 cgtcggagac gcgcgccgcc tgctgcatgt tgtgcgtcac gatgacgatc gtgaagcgct
      121 ccttcagggc gaagatgagc tcctcgatcc tggcggtcgc gatcgggtcg agggccgagc
      181 agggctcgtc catcaggacg acctccggct cgacggccag cgcccgcgcg atgcagaggc
      241 gttgcatctg cccgccggag agggcgagcg cgctctcgtc gagccggtcc ttcgcctcgt
      301 cccagagagc ggcgccccgg agcgccgact cgcacgcgtc gcgcaggacg gtcttgttcc
      361 tctccccctg gatccggagg ccgtagacga cgttctcgaa gatcgacttc gggaacgggt
      421 tcgacttctg gaagaccatc ccgagccgct tgcgcacgct cacgacgtcg acgtccggcg
      481 cgtagaggtc ctccccggcg acccggagcg agccctcctg ccggacgccg tcgatcaggt
      541 cgttcatccg gttcaggcac cggagcaggg tcgacttccc gcagccggag ggcccgatga
      601 aagcggtgac ggagcgctcg cggaccttca ggtcgatgtc gaagagcgcc tgcttcggcc
      661 cgtaccagaa gctgaacttc tcgagctcga tcagcggcgg cgcggccacg ggcgcggcct
      721 ccggacccgg ggcggtccgg accgcgagcg gcgttgcgga gacgggcgga gccatcgtct
      781 gggcttccat gatgacgtac ctcagatccc ggaggaacgg agcttccggc ggagccgtcc
      841 ccggatgacg atcgccgtga ggttgagggc aacgacgatg aggatgagga ggatcgtcgt
      901 cgtgaagacc atcgggcggg ccgcgtcgac gttcggcgac tggaacccga cgtcgtagat
      961 gtggaacccg aggtgcatga acttccggtc gaggtggaag aagggggcga agccgtcgac
     1021 cgggagcgac ggcgccagct tcacgacgcc ggtgatcatg agaggcgcca cctcgcccgc
     1081 cgcgcgggcc atcgcgagga tgaggccggt caggatcgac ggcatgaccg ccggcaggac
     1141 gacccgccac atcgtctcga acttcgtggc gccgagggcg agcgaggcct cgcgcatccc
     1201 gcgcgggatc gcggcgagcc cctcctccgc ggagacgatg acgaccggca ccgtcaggag
     1261 cgagagcgtc agcgacgccc agaggattcc gcccgtcccc cacgtcggcg ccgggagcgc
     1321 ctccgggtag aagaggcggt cgatcgagcc gccgacgaag tagatgaaga agccgaggcc
     1381 gaagacgccg tagacgatcg acgggacccc ggcgaggttg ttcacggcga tccggaccgc
     1441 gctgacgacc ggcccctgct tcgcgtactc gcgcaggtag aacgccgtca ggacgccgag
     1501 gggcgtcacg acgacgctca tgatcaggac catcaggacc gtcccgaaga tcgccgggaa
     1561 gaccccgccc tcggtgttcg actcgcgcgg gtcctcggtc acgaagcccc agacgttcgc
     1621 ggcgtagcgc cccgccttcg cgagccgccc catcccgttc acgcggtcgg cccggacgac
     1681 ctgcgagagc ggcagctcct tcgtcaggcc tcccgcggtc tcgaggacga ggatggcctt
     1741 cacccgggcc cggaccgcct gcagctcggt gaagagcctc tcgtactcgg cctggagcgc
     1801 ccgctcctcc gcgtcgagcg cgtcccgctt cggcccgccc gccgctcctt cgagctcgag
     1861 cttcttccgc tcgaggcgga gcttctcctt ctcgaagttc acggccccga tgggcccctt
     1921 ctcgagccga agcgcctctt cgtggacctc gcgcgcccgc ggcaggagct ccgcgacctt
     1981 ccgccaggag gcctcgcgcc ccgaggcgag ggacgcctca ccgtcccgca cttccttcac
     2041 ccagccgtag aagtcgcccc actcccgccg ctcgaggacg acgaggtcgg cgggggtcgc
     2101 ccgcgaccgg atggacgcct cgtcgaccca cacgaagtcc tcgccggtca ggtcgcggtt
     2161 cccctgcttg accttgatgc gccatcggtc cggcgtcccg ggggcggcgt cggggtccgg
     2221 gatcctctcc cgggccgcca cgcgcccggc gagggcgccc ccgtcctcga gcgtgaagag
     2281 gtggacgtcg gccggccaga agaagccgag gccgttcgcg aggacgagcc agagcagccc
     2341 gaacaccatc aggacggcga ccgcgaggcc gctcccggtc acccagatga agggttcccc
     2401 gctccggaag atgccgctct tccgcgcgag agacggtctc ccctcggacg cgctcattcg
     2461 aaggtcctgt acttcttccg gagccggagc ctcacgagct cggccaccgt gttgacgacg
     2521 aaggtcatca cgaagaggag gagggccgcg acgaagagga cccggaagag cgtcccgcct
     2581 tccggcgctt ccggcagctc gacggcgatg tttgccgaga gcgcccggaa gccgttgaag
     2641 atcgacgggt ccatgacggg cgtgttcccc gtcgccatca ggacgatcat cgtctctccg
     2701 acggcgcgcc cgaagccgat catgatcgcc gagaagatcc ccggcgaggc cgtcggcagg
     2761 accgtccgga gggccgtctg ccagcgcgtc gccccgagcg cgagggaacc agcgctcaga
     2821 cccggcggca cggccgagat ggagtcctcc gcgacggtga agatgatcgg gatgaccgcg
     2881 aagcccatcg cgagcccgac gacgagcgag ttgcgctggt cgtacgtgag cccgagcgcc
     2941 tcgagaagga agtgccggta gtcggccccg aggaggtacc gctcgaagcc cgcgccgacc
     3001 cagagggagg ccgcgccgcc cgccacgacg agcggcagca gcaggagcgc ctcccagccc
     3061 gccggaatgc gtgcccggac ccgcgccggc agcctccccc agacgacggc gcccagcgcg
     3121 gcgaacgccg cgatgacgac gggcatgacc aggaccccgg ggacgaacct ctcgacgacc
     3181 ggcgccagcc agaggccggc gatgaagccg aggacgacgc tcggaagcgc cgccatcacc
     3241 tccacgaccg gcttcacgaa cgagcggagg cgcgggtgca tgaactgtgc ggtgtagagc
     3301 gcggccgcga gcgcgagcgg gatggcgagg acgagggcgt agaaggtccc cttcatcgtg
     3361 ccgtagatga gcggcgtcag gctgagcttt gcctcgaagt cgtccgtgcc gcccgtcgac
     3421 tgccagacgt agtcgggccg gccgtagccc tcgtaccaga ccttcccgaa gagcgagcgc
     3481 caggtcacct cgggatgcgg gttcctcagc tcgaagtggt ggaggcgtcc gtcctccgcc
     3541 accgcgagga ctccgtccgc cttcggcgcg aagacgacgg cggcgagcgg cgcggtcccg
     3601 gcggagagcg cgaggagcgt cttcccggtc gtggcgtagt ggaccgcgag ggagcccgcg
     3661 gcgtcggcgg tgacgaagcc cttgtccctc cgcgacggcg agaaggcggt caccgcggcg
     3721 ccgtggctcc ggaagtcgtg catccgcgtc agccggcggc tgctcccgct ctcggacgac
     3781 acgacctgcc aggaggcgac ccggccatcg gcgtctcccg agacgagcgt ccggtcgccg
     3841 ttcagaaagc cgaggaccgt cacttcgcgg cccgcggccg acgccgggac cgtctcctcc
     3901 tgcttcgggc tctgctcgtc ccgcaggtcg agccggacga tctccccggc cgacgtcccg
     3961 gcgaacacgt cctgcccgcg aacgtcgagc gcgagcgtcc ggatctctcc gcgggcctcg
     4021 atcgggagga cgaccgagcg ggtctcgcgc gtcgtctcgc ccatcaggct cgtcctctcg
     4081 gtcacccgga cgagagtgat ctcccgcggc cccgtcgccg cgagcgcgat cgggccgtcg
     4141 ggccctcgaa cgtacttgaa gaccgcgacg ggcattccgt ccgcgttcac cgtcaccggc
     4201 tccccggggc ggacgaccgg cgtcacgcgc cgcttgccgt cctcgtacgc gacctccgtc
     4261 gagacctcca cgggatagag gcgcccgtcc gagaggccga ggacgatcag ctccggctcc
     4321 ggcgcgccgg ccttcacgac cgtcgcgtcg ccgagagccg ggagctcccg cgtgaagagc
     4381 tctccgccgg cgaccgggag cccgacgaga cccgcctgcg tcgcgacgaa cgcctcctcg
     4441 cggtactcgt cgacggagag ggcgagcggg acggccccgg ccgggaggct cccggccggg
     4501 accgtccggg cggatggcgg aacgaagagg ggccagacga ccgccacgat cacgaagagg
     4561 atcgcgagga tcgccgcgat gatcgccagg cccgagagcg tgacgaaccg ggcggcgaag
     4621 cgatccgcga ggaggcgcct cccgagcggg cgggaagtcc ccatcgccct cgcgggcgcg
     4681 ggggggccgt ccttggcgct gctctcgatc gtggcggaca cgtcgggacc tccgctctta
     4741 cttgaggagc tttgcgagct cttcctgctc gatcttcgcc gggagcggga aatagccgtc
     4801 cttgacgacg acttcctggc cgtccttcga gaggacgagc ttcaggaact ccccgacgat
     4861 cgggtcgagc ggcttgccgg gggccttgtt cacgtagacg tagaggaagc gcgcgagcgg
     4921 gtagttgccg gagtaggcct cctcgggggt ggccgggtgg cacttgtcgc ccggcttcgc
     4981 cgcgagcggg agcgcccgga cgcccgccgt cgcgtagccg atgccggagt accccatggc
     5041 gaagcgatcg acggtgaccc cctggacgac ggaggccgag cccggctgct ccttcacctc
     5101 gtccttgtag tcgccgttct tcaggacgtg ctccttgaag aacccgtacg tgcccgaggc
     5161 cgagttgcgg ccgaagaggc tgaccgggcg gttcgcccag tcgcccgtca ggccgagctg
     5221 gccccacgtc ttgacgttct ccttgtaccc ggcccgccgg gacttcgaga aggcggcgtc
     5281 gacctgcggc atcgtcaggc aggcgacggg attgtccttg ttgacgaaca cggcgagcgc
     5341 gtccacggcg acgcggacct gcgtcggctt gtagccgaac ttcttctcga actcgtcgat
     5401 ctcgctcccc ttcatcgcgc gcgacatcgg cccgagctgg gccgtggcgg agatgagcgc
     5461 cggcggagcc gtcgaggagc ccttcccctc gatctggatc ttcacgttcg ggtacatcct
     5521 gttgaacttc tcggcccaga gcgtcatcag gttgttcagc gaatcggagc cgatcgagct
     5581 gacgttcccc gagaccccgg aaacggcctt gtagggcttg atggccgggt cgacctggat
     5641 cgtctgcgcg gcggccgtgc cggcgagcag cgcggcggcg gcgagggtgg cggtcttctt
     5701 catcagattc ctccttcggg attccggacc gacggggagt cttcccgagg gcggccggga
     5761 cgttgttacg gccaggtaaa ttccgtgtga agcggcgctc ccgagcggcg cgtctcttta
     5821 cacggccttc actccgcctc cacgggaccc tccgacgcgc ccccgagact cgcggcgctc
     5881 tggagagacc cgaaaggaga gacggaatgg atcgcacgct tcgacgcctc gcgggattcg
     5941 ccctcgcagg cattctgctc ctcgccggcg ccgccccggc ccaggtgaac cttctctggt
     6001 acaaggaggt cgcgaaggac ggccggatct acgtcttcaa cgacccgaac gagttcaagg
     6061 cctgggaagg ctcgggcgag ctcggcaagt cgatcacgaa gatcggcttc ggcccgaacg
     6121 gcgagacggt cgtcttcgac aacgacacgg ccctcgacct ctacgtcttc aagcacggca
     6181 aggagcccga ggtccggcct tactccgcgc cgaagccccc gaagcccgtc ccgcccacga
     6241 cgctgaagat cggggacggc gagctgaagc tcggcggcct cctccagggc tggtacgtcc
     6301 tggacgactc ggcgaccggg agcggctcga gccagctcgg caactccacc ggcgtcaaca
     6361 cgttccgcct tcgccgcgcc gagatcaagc tctcgggcaa ggtgaccccg gagtggggct
     6421 tcgaggtcat gatcgacccg acgaagagcc cgagcgtcac cgcc
//
LOCUS       DAOTEZ010000236         6460 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_8573_length_6460_cov_7.284778, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000236 DAOTEZ010000000
VERSION     DAOTEZ010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6460
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8573_length_6460_cov_7.284778"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="P5164_12625"
     CDS             <1..999
                     /locus_tag="P5164_12625"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRY44779.1"
                     /translation="GVFVLPALAWVLFLKFFERPMGRLHRVLLTAQAAGALACLFWPR
                     PDDIYYTFPLGGLLGAAAAADVVAHLGRAARRRVRHARPLLAAALAAVLAAAFDSAGL
                     LALGVGASIDIALSGPAFFVLMSVLLAALADRLARLRLAASTDPLTSLANRAVLFDRI
                     RLEIARARRSAHPVALAILDLDGFKGFNDRYGHVAGDRLLVAVAAAITEAIRDTDLAA
                     RFGGDEFVVLLPETNATRAVACLERIREGIARARVSGTRDGTTASAGVAVFDPNVRAS
                     VSVSAWLRQADAALYAAKTAGRDRVLVAHGAPPAAASGDRVPSDDASIRRVPGSPP"
     gene            996..1727
                     /locus_tag="P5164_12630"
     CDS             996..1727
                     /locus_tag="P5164_12630"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRY44780.1"
                     /translation="MTGGAGAGAPSATGRVLLDDGSVLALDKPAGVSMATSSRPGKSE
                     EEVVGRLLAACGAPDGAPRPLLVHRLDEGTSGVVLLARGAAMHRLLSAAFQGRVARKT
                     YRALAWGHPVPARGLADAPLARDPKDGRRMVVREGGKPARTRWATLRRYSGVADLEVA
                     PETGRTHQIRVHLAALGHPLVGDDLYGGGTRWRGVRDPALRAAFAAVTHPLLHAARVA
                     VPSLGVDVEATLPGDYSALLALLAR"
     gene            complement(1740..2975)
                     /locus_tag="P5164_12635"
     CDS             complement(1740..2975)
                     /locus_tag="P5164_12635"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY44781.1"
                     /translation="MSLRESLLRALASLSAHKLRSVLTMLGMIFGVGAVIAMLSIGAG
                     AESQALEMIRRMGISNVLVRAKEFRPDELQEVRKKSIGVSLRDAAGIEEAVPGVDFTG
                     PRVTVNPWSVRGRGGKSEARVSGVSWRHAELVSLPLAAGRFLTKEDEETHAQVCVLGD
                     GARRDLFGWEPALGRTVKVNDVWLSVVGVLAPSGEGGDTFQGVSVGSTAQELFVPYTT
                     VLRKLDRDPLESPLSELVVKLRDGTSATEAARLAGALLDRLHGGAADFEVVVPEALLA
                     QSRKTQRLFNVVMGCIAGISLLVGGIGIMNIMLATVLERTREIGIRRAIGARRKDVLV
                     QFVVEAFALSALGGLLGVGLGVLIAKVVAASAGWPTVVTLWSLLLSTGVSGAVGLLSG
                     FYPALRAASIDPIEALRYE"
     gene            complement(2972..4234)
                     /locus_tag="P5164_12640"
     CDS             complement(2972..4234)
                     /locus_tag="P5164_12640"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRY44782.1"
                     /translation="MRRRTLVVLAAALLAAAALGARLLRPRAPDAGVPTFVVKAAKLE
                     RSVRAEGVLRAVDATPVRVPGNDVSLKVGWMIEDGTPVKAGDVVVRLDPTQTTKLLAD
                     GRAERATADRRGDKERAEAGALIANLRRDADQARDERTTAETFQSRDPELFSRHEIIE
                     ADIDVSLATQKERHAVGSKGEREALARTGRELVALDGRTAQLKIDKAERELAALELKA
                     PHDGIVVLKRNWRGELPQVGQVVWSSQTLAEIPDLGKLEAEVWVLEADAGGLAPGRPA
                     TLSLEGEAGRPVPAKVKSVDALARPRLRNVPIQYFGAVLSLDAVDPARMKPGQRVAAT
                     LDLGAEKDALVVPRQALFERGGRTVVFRLAGNAFESVPVTLGASALGRVAVASGLREG
                     DVVALVDPEAAPEPAAPSASGPPVGGAS"
     gene            complement(4231..>6460)
                     /locus_tag="P5164_12645"
     CDS             complement(4231..>6460)
                     /locus_tag="P5164_12645"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY44783.1"
                     /translation="RGMKRGRLSRLALAVVLLLAAASVLLGCRGARAGDGPLPATPDA
                     DGLRPLLLTGELAALRSADLFVPETPLWELQLRFLEEDGAAVKAGDRVAEFDAGNLAS
                     ELEQKRIAAIEAETELVRFDADREVAELAREQAVLARTTDLEKARLDADLPPELRSRR
                     DHEEKQLALRRARTELEKAREDLAGYRAAAAADRATKLIALERSRAERDKLEAAVAAL
                     TLRAPKDGVFLVAENPRERKKFLTGDTLWPGLALARIPDPGALRVDALLFDVDDGRVR
                     AGDAAVVVPDTFPDRRLPARVASVAPVAQPIGRGSPRMAFRVQVDVTGGELGGLRPGM
                     SVRVETPPAPTGSVAADPSPVPLSPPDLASARRTKAEMRDLVLSVELKGELAALDGEP
                     IGPTRLPDVWDYRISFLAPEGSEVKKGQPVLRFDTTAVSQQLEEKRAEAESAAKQIER
                     RRRTLALSRADLELQKAQAEARRAKAALKVDVPPEISGAIELTKARLDLALAEAESAS
                     VTRRLEAAERAARAELSILEGRRRRAALRAGELTTAIGKMTVPAPRAGTVLLIPSWNG
                     DKKKVGDRCWFGEKILEVPDLARLGVQGEVDEADAGLVREGASVSLRLDAHPDSEVKG
                     KVLRIQRTVQRASPSVPLKVARLSVALETVDPKKMKPGMRVRGRVETGRAARALAVPA
                     DAVVPTEAGPFVWVPRGSGARAVAVTVGRRNGEWVEVLSGLAEGDAVLRVARPAPKGA
                     AS"
BASE COUNT          762 a         2422 c         2448 g          828 t
ORIGIN      
        1 ggcgtcttcg tcctgccggc cctcgcctgg gtcctcttcc tgaagttctt cgagcggccg
       61 atgggccggc tccaccgcgt cctgctgacg gcgcaggcgg ccggagcgct ggcgtgcctc
      121 ttctggccga ggcccgacga catctactac acgttcccgc tgggcggcct cctcggcgcg
      181 gctgcggcgg cggacgtcgt cgcccatctc ggcagggcgg cccggcggcg cgtccggcac
      241 gcgcggccgc tcctcgccgc ggcgctcgcg gcggtcctgg cggcggcgtt cgactccgcc
      301 ggcctcctcg ccctcggcgt cggggcctcg atcgacatcg ccctctccgg gcccgccttc
      361 ttcgtcctga tgagcgtcct cctcgcggcg ctcgccgacc gcctcgcgcg cctccggctc
      421 gcggcctcga ccgatcccct cacctcgctc gcgaacaggg cggtcctctt cgaccggatc
      481 cggctcgaga tcgcccgggc ccggaggagc gcccacccgg tggcgctcgc gatcctcgac
      541 ctcgacgggt tcaaggggtt caacgaccgg tacggccacg tggccggtga ccgcctcctc
      601 gtcgcggtcg ccgccgcgat cacggaggcc atccgggaca ccgatctggc ggcccggttc
      661 ggcggcgacg agttcgtcgt ccttctgccg gagaccaacg ccacccgggc cgtcgcctgc
      721 ctcgagagga tccgggaggg catcgcacgt gcccgggtct ccgggacgcg ggacgggacg
      781 acggcctccg cgggcgtcgc cgtcttcgac ccgaacgtcc gggcgagcgt ctcggtctcg
      841 gcctggctgc ggcaggcgga cgccgcgctc tacgccgcga agacggcggg ccgggaccgc
      901 gtcctcgtcg cgcacggcgc tccgcccgcg gcggcctccg gggatcgtgt cccgagcgac
      961 gacgcgtcga tccgccgcgt cccgggctct ccgccgtgac cggaggcgcc ggcgccggag
     1021 ctccgtccgc gacgggccgc gtcctcctcg acgacgggtc ggtcctcgcc ctcgacaagc
     1081 ccgccggcgt ctcgatggcg acctcctcgc ggccgggaaa gtcggaggag gaggtcgtcg
     1141 gacggctcct cgccgcctgc ggagcgccgg acggggcgcc gcgcccgctc ctcgtccacc
     1201 ggctcgacga ggggacctcg ggtgtcgtcc tcctggcgcg gggcgcggcg atgcaccggc
     1261 tcctgtcggc ggctttccag gggcgcgtcg ctcggaagac ctaccgggcg ctcgcgtggg
     1321 gccacccggt cccggcgcgc gggctcgccg acgcgcccct cgcgcgcgac ccgaaggacg
     1381 ggcggaggat ggtcgtccga gaaggcggca agcccgcgcg gacgcgctgg gcgacgctcc
     1441 gccgttactc cggcgtcgcg gacctcgagg tcgcgcccga gacgggccgg acccaccaga
     1501 tccgcgtcca cctcgcggcg ctggggcatc cgctcgtcgg cgacgacctc tacggcggcg
     1561 gcacgcgctg gcgcggcgtc cgcgacccgg cgctccgggc cgcctttgcc gcggtgacgc
     1621 acccgctgct tcatgccgcc cgcgtcgccg tcccctcgct cggggtcgac gtcgaggcga
     1681 cgctccccgg ggactattcc gccctcctcg ccctcctcgc gcgctgacgg cggggcgggc
     1741 tactcgtacc ggagcgcctc gatcgggtcg atcgaggcgg cgcgcagcgc cgggtagaag
     1801 ccggagagga ggccaacggc gccggagacg ccggtcgaca ggaggaggga ccagagcgtc
     1861 acgacggtcg gccagccggc ggaggcggcg acgaccttcg cgatgaggac cccgagcccg
     1921 acgccgagga ggcccccgag ggcgctcagc gcgaacgcct cgacgacgaa ctgcacgagc
     1981 acgtccttcc gccgcgcgcc gatcgcgcgg cggatgccga tctcccgcgt ccgctcgagg
     2041 acggtcgcga gcatgatgtt catgatcccg atccccccga cgaggagcga gatcccggcg
     2101 atgcacccca tgacgacgtt gaagagccgc tgggtcttcc ggctctgggc gaggagcgcc
     2161 tccgggacga cgacctcgaa gtcggccgcg ccgccgtgga gccggtcgag gagcgccccc
     2221 gcgaggcggg cggcctccgt ggcggaggtc ccgtcgcgga gcttcacgac gagctcggag
     2281 agcggggact cgagcgggtc gcggtcgagc ttgcggagga ccgtcgtgta cgggacgaag
     2341 agctcctgcg cggtggagcc gacggagacc ccctggaacg tgtcgccgcc ttcgcccgag
     2401 ggggcgagga ccccgacgac ggagagccag acgtcgttca ccttcaccgt ccgaccgagc
     2461 gcgggctccc agccgaagag gtcgcgccgg gcgccgtcgc cgaggacgca gacctgcgcg
     2521 tgcgtctcct cgtcctcctt cgtgaggaag cgccccgcgg cgagagggag cgagacgagc
     2581 tcggcgtggc gccacgagac gcccgagacg cgcgcctcgc tcttcccgcc gcgtccccgc
     2641 acgctccacg ggttcacggt gacgcgcggc ccggtgaagt cgacgcccgg cacggcctcc
     2701 tcgatcccgg ctgcgtcgcg gagcgagacg ccgatcgact tcttccggac ctcctggagc
     2761 tcgtcgggac ggaactcctt cgcgcggacg aggacgttcg agatccccat ccgccggatc
     2821 atctcgagcg cctggctctc ggcgccggct ccgatcgaga gcatcgcgat gacggcgccg
     2881 accccgaaga tcatcccgag catcgtcagg acggaccgca gcttgtgcgc cgagaggctc
     2941 gccagggcgc gcaggagcga ctcgcggagg ctcacgacgc ccctccgaca ggggggccgg
     3001 aggcggaggg agcggccggc tcgggcgccg cctcggggtc gacgagcgcg acgacgtcgc
     3061 cttcgcgcag gcccgaggcg acggcgacgc gcccgagggc ggaggcgccg agcgtgaccg
     3121 ggacggactc gaaggcgttc cccgcgagcc ggaagacgac ggtccggccg ccgcgctcga
     3181 agagggcctg cctcgggacc accagggcgt ccttctccgc cccgaggtcg agcgtcgccg
     3241 cgacgcgctg tcccggcttc atccgggcgg ggtcgaccgc gtcgagggag aggaccgcgc
     3301 cgaagtactg gatcgggacg ttccggaggc gcggccgcgc gagcgcgtcg accgacttca
     3361 ccttagcggg gacgggccgg ccggcctcgc cctcgagcga gagcgtggcg ggacgcccgg
     3421 gcgcgaggcc cccggcgtcc gcctcgagga cccagacctc ggcctccagc ttcccgaggt
     3481 cggggatctc cgcgagggtc tgggagctcc agacgacctg cccgacctgg ggcagctcgc
     3541 cgcgccagtt ccgcttcagg acgacgatcc cgtcgtgcgg ggccttcagc tcgagcgcgg
     3601 cgagctcgcg ctcggccttg tcgatcttca gctgcgccgt gcgtccgtcc agggcgacga
     3661 gctcccgccc cgtccgcgcg agcgcctcgc gctccccctt cgagccgacg gcgtggcgtt
     3721 ccttctgcgt cgcgagggag acgtcgatgt ccgcctcgat gatctcgtgc cgggagaaga
     3781 gctccgggtc gcggctctgg aacgtctcgg cggtcgtccg ctcgtcgcgg gcctggtcgg
     3841 catcgcgacg gaggttcgcg atgagggctc ccgcctcggc gcgctccttg tcgccgcgtc
     3901 ggtccgccgt cgcgcgctcc gcccggccgt cggcgaggag cttcgtcgtc tgcgtcgggt
     3961 cgaggcggac gacgacgtcg cccgccttca cgggcgtccc gtcctcgatc atccagccga
     4021 ccttcaggct cacgtcgttt cccgggaccc ggacgggcgt cgcgtccacg gcccggagga
     4081 ccccctcggc gcggacgctc cgctcgagct tcgccgcctt cacgacgaac gtcgggacgc
     4141 cggcgtccgg cgcccgcggc cggaggagcc tcgccccgag ggccgcagcg gcgaggagcg
     4201 cggcggcgag gacgacgagg gtccgccgtc tcacgaggcg gctcccttcg gcgccgggcg
     4261 ggcgacgcgc aggacggcgt ccccctcggc gaggccggag aggacctcga cccattcgcc
     4321 gttgcgccgc ccgaccgtca cggcgacggc gcgggcgccg cttccgcggg ggacccagac
     4381 gaaggggccg gcctcggtcg ggacgaccgc gtccgccggg acggcgaggg cacgcgcggc
     4441 gcgcccggtc tcgacgcggc cgcggacgcg catccccggc ttcatcttct tcgggtcgac
     4501 cgtctcgagc gcgacggaga ggcgcgcgac cttcagcgga acggaaggcg aagcccgctg
     4561 gaccgtcctc tggatccgaa ggaccttccc cttcacctcg gagtcggggt gggcgtcgag
     4621 acggagcgag accgacgccc cctcccggac gaggccggcg tcggcttcgt cgacctcgcc
     4681 ctggacgccg aggcgggcga ggtcggggac ctcgaggatc ttctcgccga accagcagcg
     4741 gtccccgacc ttcttcttgt cgccgttcca gctcgggatg aggaggacgg tccccgcgcg
     4801 cggggcgggc acggtcatct tcccgatcgc cgtcgtcagc tcgccggccc ggagcgcggc
     4861 gcgccggcgg cgcccctcga ggatcgagag ctcggcgcgt gccgcgcgct cggccgcctc
     4921 gaggcggcgc gtgacggagg ccgactccgc ctccgcgagc gcgaggtcga ggcgcgcctt
     4981 cgtcagctcg atcgcgccgg aaatttccgg ggggacgtcg actttcagag ccgccttcgc
     5041 gcggcgcgcc tcggcctggg ccttctgcag ctcgaggtcg gcgcgggaga gcgcgagcgt
     5101 ccggcgccgc cgctcgatct gcttggccgc cgactcggcc tccgcacgct tctcctcgag
     5161 ctgctgggag accgcggtcg tgtcgaagcg gaggacgggc tgccccttct tcacctcgga
     5221 gccctcgggc gcgaggaagg agatcctgta gtcccagacg tcggggaggc gcgtggggcc
     5281 gatcggctcg ccgtcgagcg cggcgagctc gcccttcagc tcgacggaga ggacgaggtc
     5341 gcgcatctcg gccttcgtgc ggcgcgcgct cgcgaggtcg ggaggtgaga gcgggacggg
     5401 gctcgggtcg gccgcgacgg accccgtcgg cgcgggaggc gtctcgacgc ggacggacat
     5461 gccggggcgg agcccgccga gctcccctcc ggtcacgtcg acctggacgc ggaaggccat
     5521 ccgcggcgaa ccgcgcccga tcggctgcgc gaccggggcg acggaggcga cccgggccgg
     5581 aagacggcgg tcggggaagg tgtcggggac gacgacggcg gcgtcgcccg cacggacccg
     5641 gccgtcgtcg acgtcgaaga ggagcgcgtc gacgcggagc gccccggggt cggggatgcg
     5701 cgcgagcgcg aggccgggcc agagcgtgtc gcccgtcagg aacttcttcc tctcgcgcgg
     5761 gttctcggcg accaggaaga cgccgtcctt cggcgcgcgg agagtgaggg cggcgacggc
     5821 cgcctcgagc ttgtcgcgct cggcgcgcga gcgttccagg gcgatgagct tcgtggcgcg
     5881 gtcggccgcc gccgccgcgc ggtacccggc gaggtcctcc cgggccttct cgagctccgt
     5941 ccgcgcgcgg cggagggcga gctgcttctc ctcgtggtcc cgccgggagc ggagctcggg
     6001 cgggaggtcg gcgtcgagac gggccttctc gaggtcggtc gtccgggcga ggacggcctg
     6061 ctcccgtgcg agctcggcga cctcccggtc cgcgtcgaag cggacgagct cggtctccgc
     6121 ctcgatcgcg gcgatccgct tctgctcgag ctcggaggcg aggttcccgg cgtcgaactc
     6181 ggcgacgcgg tcgcccgcct tcacggcggc gccgtcctcc tcgaggaagc ggagctggag
     6241 ctcccagagc ggcgtctcgg ggacgaagag gtcggccgag cgaagggcgg cgagctctcc
     6301 cgtcaggagg agcggccgga ggccgtcggc gtccggcgtc gccgggaggg ggccgtcgcc
     6361 ggcccgggcg ccgcggcagc ccaaaagaac gctcgcggcg gccagcagga gcaccaccgc
     6421 gagcgcgagc cgggagagtc gtccccgttt cattccccgc
//
LOCUS       DAOTEZ010000237         6457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_8575_length_6457_cov_5.942362, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000237 DAOTEZ010000000
VERSION     DAOTEZ010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8575_length_6457_cov_5.942362"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(365..952)
                     /locus_tag="P5164_12650"
     CDS             complement(365..952)
                     /locus_tag="P5164_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44784.1"
                     /translation="MRTHVHSFRMTGLLFLAALVALPAFAAGKRFQDDDASREKDEPT
                     TWLKDYDKLVEGKEADWVFFDGYSAGSFKSVAVKEFSASEKDKEARHAAEYGKEYAEQ
                     WIQESKKLGWEVVKDGKADLTIEGNVAHAWEPSGAARFWGGWYANPGAVQELIGKDAS
                     GKIVFQIRHKSRGSTVKDAIENGLEDVVKSLEKGK"
     gene            1115..2911
                     /locus_tag="P5164_12655"
     CDS             1115..2911
                     /locus_tag="P5164_12655"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRY44785.1"
                     /translation="MKKIRASTLALGLSLVVSFLALAQAARREASAPPARSPRLAVLV
                     SVDGVQMKRLLDYRPYFVAGLKQLLDEGHVERNAHYAHLNTETGPGHASLGTGAPPRV
                     TGIVANRWFEPRADGSLRPVYCTDQPYVDPETGVDAFRPGPGNLRVPTLGDRLLEKYP
                     ASRVVSLSGKDRGAIFMAGKRREHAVYWWDQRTGRFVSSPAYDVRSPAGAVVSGIVER
                     YNRTRAGGHLPRRIGLSWRKMADPACLPPPSPATARPVPELEMRPFQIPVNGLGWDKD
                     MSLASNGYFHGLYYSPFIDELTIDLALEVLASKELELGHEEQPDLLCLSLSGQDTVSH
                     AYGAESEENLDALRRLDVQLGRLLEALGRTFPDGKVVLALSADHGFQTIPEREARVDR
                     TFTGGRILTGSGAVTNFEERLNRYVCEELCLPPGSRPIFGNEGFDVKYNLPALPAMRT
                     AAGPCGEAGRAVTRDDVDRVLPGAIARLWHEEFRTVLLASRKESWDRNDRDVRFALND
                     YDAERSGEALLIPRRGVILYPDARGGTHGSQYVYDTHVPLLFWGGGVAAGVSDAERTP
                     YDFAPTVGKLLGVALPDAVGTAIELPRPTAKP"
     gene            2977..4047
                     /gene="rsmH"
                     /locus_tag="P5164_12660"
     CDS             2977..4047
                     /gene="rsmH"
                     /locus_tag="P5164_12660"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005032369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRY44786.1"
                     /translation="MDEPRPRRRPRYPGRNPRRFEEKYKERDPARFPETVAKVVASGK
                     TPAGTHRPVLVAEVLDALRPAPGLVAVDATLGYGAHAAALLPRLLPGGRLVGLDADPV
                     ELPRTEARLRDLGFGEEVLTVRRSSFAGLSKVLSGLGLETVDLLLADLGVSSMQLDDP
                     ARGFTFREDGPLDMRMNPVRGLSAAQLLARVSEARLAALLEENADEPHAAAIARAVVT
                     ARGLRPVDTTAALADAVRRALSALPERVRQEEGDDPVRQTFQALRIEVNDELGALRAL
                     LTALPGCLAPGGRVAILTFHSGEDRLVKKAFQAGARAGVFSDWSREPVRPGPEEVRAN
                     PRARPAKLRWALRAETGDLPAF"
     gene            4057..5394
                     /locus_tag="P5164_12665"
     CDS             4057..5394
                     /locus_tag="P5164_12665"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY44787.1"
                     /translation="MLTAECATSRPLLGSARWEWWDWWLERKTRWVRDEVKQLDEGVF
                     RTLAAGTGLPAIHQRSTFPFKDVRDGADRFLGMAKGGERPYARIYTRLGNPTTEYLER
                     VLFQLEAHHVIEKALAVDEKEPTIGALIFGSGMGAISTLALAVCRAGDAILAGNVYGC
                     TDSLFRGLGKFGVEAVFCDMGNVAAVEAALDAHPNVAMVFLESPENPTLRIADIEAIS
                     RLTEPRGVLLVVDNTFCSPYLQQPFRLGADLVMHSLTKFVNGHSTSIGGALLGPFRFM
                     KTDFFPWYKDVGATPSPFDSWLNGMTVQSLAPREAQQSATALEIARFLSTHPKVASVG
                     YPGLPDFPQADLVRRQMRSGGAILTFEVKGGVSAGETVMNYFGRKDTPMELAVSLGSC
                     ITYIQHPASMTHAVVPEADRLARGITPGLIRLSVGLEGAEVLVEHLGRALDLT"
     gene            5438..>6457
                     /locus_tag="P5164_12670"
     CDS             5438..>6457
                     /locus_tag="P5164_12670"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HRY44788.1"
                     /translation="MARVRNIAINTGGGDAPGLNAVICGVVSAALNRGWRCFGIRDGY
                     NGLLFPEQYPDGGLVSLSRTRVEGIASLGGTILGTTNRGNPLRFPVRQPDGTTIEVDR
                     TDELVRAFALHEIDALVSIGGDGSLAIANVLAKKGLRVVGVPKTIDNDLDRTVITFGF
                     DTARSFATEAIDRLHSTAEAHRRIMVVEVMGRYAGWIALDSGVAGNADAILIPEIPYD
                     LEKVAEHLRRTEESGQRHAIVVVAEGAKPKGGTVSTLGPREAGAMERLAGAGERVMKG
                     LEELTGREVRTVVLGHLLRGGSPSSFDRLLALRFGAAAVRALESGQSGVMVALAPPTV
                     NYVPLE"
BASE COUNT          880 a         2446 c         2185 g          946 t
ORIGIN      
        1 ctctccgagg ggaagaagac cgacgagatg ggcgagcagc tcttcatcag cgacgtcacc
       61 gtgaggaacc acatccagaa catcctccgg aagctgaacg tccactcgaa gctcgaggcc
      121 gtcgtctacg cgtaccagaa ccagctgatc tgagctgaag tgttcccggg gggaccccgc
      181 gcctgcggcg cggcggccgg tccccccgga ccccctccga gccgcctctc ctctcttccc
      241 gcacgtcgtt ccgccgcggg gaccccgcgc ctgcggcgcg gcggccggcc ccccgggaaa
      301 cgcgaaagcg gcggccgggg ccgccgcttt cttcacccgc gggtgttctc cggcaccccg
      361 gaggtcactt ccccttctcg agcgacttga cgacgtcctc gagcccgttt tcgatggcgt
      421 ccttcaccgt ggagccgcgg gacttgtggc ggatctggaa gacgatcttc ccggaggcgt
      481 ccttgccgat cagctcctgg accgctcccg ggttcgcgta ccagccaccc cagaaacgcg
      541 ccgcgccgct cggctcccag gcgtgcgcga cgttcccttc gatcgtcagg tcggccttgc
      601 cgtccttcac gacctcccag ccgagcttct tcgattcctg gatccactgc tcggcgtact
      661 ccttgccgta ctcggcggcg tggcgcgcct ccttgtcctt ctcggacgcg gagaactcct
      721 tcacggccac gctcttgaag ctgccggccg agtagccgtc gaagaagacc cagtcggcct
      781 ccttcccctc gacgagcttg tcgtagtcct tgagccaggt cgtcggctcg tccttctcgc
      841 gggaggcgtc gtcgtcctgg aaccgcttcc ctgcggcgaa ggccggcagc gcgacgagcg
      901 cggccaggaa gaggaggccg gtcatccgaa acgagtgcac gtgcgttctc atggcgcgaa
      961 tgctccttca gtgcgttcgt gttttccgtg atcttagcaa cacgttcgac gctttcggga
     1021 atgggccccg cggcagggcg cggccccggg tccggctccc gcccgcgctt agaatcgcct
     1081 ccggcgctcc cttcccgggt ccgcccccag cagaatgaag aagatccgcg cctcgactct
     1141 cgccctcggg ctctcgctcg tcgtctcgtt cctcgcgctg gcccaggccg cgcgccgcga
     1201 agcctccgcg cccccggccc ggtcgccgcg tctcgccgtc ctcgtctcgg tggacggcgt
     1261 ccagatgaag cgcctgctcg actaccggcc ctacttcgtc gccgggctga agcagctcct
     1321 cgacgagggg cacgtcgaga ggaacgcgca ctacgcccac ctgaacaccg agacggggcc
     1381 gggtcacgcc tcgctcggca cgggcgcccc gcctcgcgtc accggcatcg tcgcgaaccg
     1441 ctggttcgag ccgcgcgccg acggctctct ccgccccgtc tactgcaccg accagcctta
     1501 cgtcgacccg gagacgggag tcgacgcctt ccgccccggg ccgggcaacc tgagggtccc
     1561 gaccctcggc gaccggctgc tcgagaagta cccggcctcg cgcgtcgtct cgctctcggg
     1621 caaggaccgc ggcgcgatct tcatggccgg gaagcggcgg gagcacgccg tctactggtg
     1681 ggaccagagg accggacgct tcgtcagctc ccccgcctac gacgtccgct cgccggcggg
     1741 cgccgtcgtc tccgggatcg tcgagcgcta caaccggacg cgggccggcg ggcacctgcc
     1801 gcggcggatc ggcctttcct ggcggaagat ggccgacccc gcctgcctcc cgccgccgtc
     1861 gccggcgacg gcccggccgg tcccggagct cgagatgcgt ccgttccaga tccccgtgaa
     1921 cggcctcggc tgggacaagg acatgtccct cgcctcgaac ggctacttcc acggcctcta
     1981 ctacagcccg ttcatcgacg agctgacgat cgacctcgcg ctcgaggtcc tcgcctcgaa
     2041 ggagctcgag ctcgggcacg aggagcagcc cgacctcctc tgcctctcgc tctcggggca
     2101 ggacaccgtc tcccacgcgt acggcgccga gtcggaggag aacctcgacg cgctccggcg
     2161 gctggacgtt cagctcgggc gcctgctcga ggcgctcggg aggacgtttc ccgacggcaa
     2221 ggtcgtcctc gcgctttccg ccgaccacgg cttccagacg atccccgagc gcgaggcccg
     2281 ggtcgacagg acgttcacgg gcggccgcat cctgacggga agcggcgccg tgacgaactt
     2341 cgaggagcgc ctcaaccgct acgtctgcga ggagctctgc ctcccgcccg gctcgcgccc
     2401 gatcttcggg aacgagggct tcgacgtgaa gtacaacctc ccggccctgc cggcgatgcg
     2461 gaccgccgcc ggcccgtgcg gcgaggccgg gcgggccgtg acgcgggacg acgtcgaccg
     2521 ggtcctcccc ggggcgatcg cccgcctctg gcacgaagag ttccggacgg tcctcctcgc
     2581 ctcgaggaag gagagctggg accggaacga ccgcgacgtc cgtttcgcgc tgaacgacta
     2641 cgacgcggag cggtcgggcg aggcgctcct gatcccccgg cgcggcgtga tcctctaccc
     2701 cgacgcgcgc ggcgggaccc acggctccca atacgtctac gacacgcacg tcccgctcct
     2761 cttctggggc ggcggcgtgg cagccggggt ctccgacgcc gagcgaaccc cgtacgattt
     2821 cgccccgacc gtcggaaagc tcctcggggt ggcgctcccc gacgccgtcg ggacggcgat
     2881 cgagcttccc cgcccgaccg cgaagccctg agagtccggt ccgccgcgac ggggccgtcg
     2941 cggcgggccg gaccgtgacc gtcgcacaat cgacgcgtgg acgagccccg accccgccgg
     3001 cgcccgcgct atccgggacg caacccgcgc cgcttcgagg agaagtacaa ggagcgggat
     3061 ccggcccgct tccccgagac ggtcgcgaag gtcgtcgcgt cgggcaagac cccggccggg
     3121 acgcaccgtc ccgtcctcgt cgcggaggtc ctcgacgctc tccggcccgc gcccggcctc
     3181 gtcgccgtcg acgcgacgct cggatacggc gcccacgccg cggcgctcct gccgcgactc
     3241 ctcccgggcg gccggctcgt cggactcgac gcggaccccg tcgagctccc gaggaccgag
     3301 gcgcgtctgc gcgacctcgg gttcggcgag gaggtcctca ccgtccgccg gtcgagcttc
     3361 gcagggctct cgaaggtcct ttccggcctc gggctcgaga cggtcgacct cctcctcgcg
     3421 gacctcggcg tctcctcgat gcagctcgac gacccggcgc gcggtttcac gttccgcgag
     3481 gacgggccgc tcgacatgcg gatgaacccc gtccgcggcc tctccgccgc gcagctcctt
     3541 gcgcgcgtct ccgaagcgcg cctcgccgcg ctcctcgagg agaacgccga cgagccgcac
     3601 gcggcggcga tcgcccgggc ggtcgtcacc gcacgcgggc tccggccggt cgacacgacc
     3661 gcggccctcg cggacgccgt ccgtcgggcc ctctccgcgc tgccggagcg cgtccggcag
     3721 gaggaggggg acgaccccgt gcggcagacg ttccaggcgc tccggatcga ggtgaacgac
     3781 gagctcggcg cgctccgcgc cctgctgacg gcgctccccg ggtgcctcgc gcccggcggg
     3841 cgcgtcgcga tcctgacgtt ccactccggc gaggaccggc tcgtgaagaa ggcgttccag
     3901 gccggggcga gggccggcgt cttctccgac tggtcccgcg agcccgttcg ccccgggccg
     3961 gaggaggtcc gggcgaaccc gcgcgcccgt cccgcgaagc tgcgatgggc tctccgtgcg
     4021 gagacgggcg atctccccgc gttctaagat cgcgccatgc tgactgcaga gtgcgcgacc
     4081 tccagacccc ttctcggatc ggcccgctgg gaatggtggg attggtggct cgaacggaag
     4141 acccgctggg tgcgggacga ggtgaagcag ctcgacgagg gggtcttccg gaccctcgcc
     4201 gcgggcaccg gcctccccgc gatccaccag cgctccacgt tcccgttcaa ggacgtccgc
     4261 gacggcgccg accgcttcct cgggatggcc aagggaggcg agcgccccta cgcccggatc
     4321 tacacccgcc tcgggaaccc gacgaccgaa tacctcgagc gggtcctctt ccagctcgag
     4381 gcccaccacg tcatcgagaa ggccctcgcg gtggacgaga aggagccgac gatcggcgcc
     4441 ctgatcttcg gctcggggat gggagcgatc tcgacgctcg ccctcgcggt ctgccgggcc
     4501 ggcgacgcga tcctcgcggg gaacgtctac ggctgcaccg acagcctctt ccgggggctc
     4561 gggaagttcg gcgtcgaggc ggtcttctgc gacatgggga acgtcgcggc cgtcgaggcc
     4621 gcgctcgacg cccacccgaa cgtcgcgatg gtcttcctgg agtcgccgga gaacccgacg
     4681 ctccggatcg ccgacatcga ggcgatctcg cgcctcaccg agccgcgcgg agtcctcctc
     4741 gtcgtcgaca acacgttctg ctcgccctac ctccagcagc cgttccggct cggcgccgac
     4801 ctcgtcatgc actcgctgac gaagttcgtg aacggccact cgacctcgat cggcggcgcg
     4861 ctcctcggac cgttccggtt catgaagacc gacttcttcc cctggtacaa ggacgtcggc
     4921 gccacgcctt ccccgttcga ctcgtggctg aacggcatga cggtccagag cctcgccccg
     4981 agggaggccc agcagagcgc gaccgcgctc gagatcgccc gcttcctctc gacgcatccg
     5041 aaggtcgcgt cggtcggcta ccccggcctg ccggacttcc cccaggcgga cctcgtccgc
     5101 cgccagatgc gctcgggcgg ggcgatcctg acgttcgagg tgaagggcgg agtctcggcc
     5161 ggcgaaacgg tgatgaacta tttcggccgc aaggacacgc cgatggagct cgccgtgtcg
     5221 ctcggctcct gcatcaccta catccagcac ccggcctcga tgacgcacgc cgtcgtcccg
     5281 gaggcggacc ggctcgcccg cgggatcacg cccggtctga tccggctctc cgtcgggctc
     5341 gagggggcgg aggtcctcgt cgagcatctc gggcgagcgc tcgacctgac ctgagctcgc
     5401 gattcgtccg ccctgggggc gggaaggggg acgcacgatg gcccgagtcc ggaacatcgc
     5461 gatcaacacc gggggcggcg acgcccccgg cctgaacgcc gtcatctgcg gcgtcgtgag
     5521 cgccgccctg aaccgcggct ggcgctgttt cgggatccgc gacggctaca acggcctcct
     5581 cttccccgag cagtaccccg acggggggct ggtctcgctg agccggacgc gggtcgaggg
     5641 aatcgcctcc ctcggcggga cgatcctcgg gacgacgaac cgagggaacc cgttgcgctt
     5701 cccggtccgc cagcctgacg ggacgacgat cgaggtcgat cggaccgacg agctcgtccg
     5761 cgccttcgcc ctccacgaga tcgacgccct cgtctcgatc ggcggcgacg gctccctcgc
     5821 gatcgcgaac gtcctcgcga agaaggggct ccgggtcgtc ggcgtcccga agacgatcga
     5881 caacgacctg gaccggacgg tcatcacgtt cgggttcgac acggcgcgat ccttcgcgac
     5941 cgaggcgatc gaccgcctcc actccacggc cgaggcgcac cgccggatca tggtcgtcga
     6001 ggtgatgggg cgctacgccg gttggatcgc cctcgactcg ggcgtcgccg ggaacgccga
     6061 cgccatcctg atccccgaga tcccgtacga cctcgagaag gtggcggagc acctgcggcg
     6121 caccgaggag tcgggccagc gccacgccat cgtcgtcgtc gccgaagggg cgaagccgaa
     6181 gggggggacg gtctccacgc tcggcccgcg cgaggcgggc gcgatggagc ggctcgcggg
     6241 cgccggggag cgcgtgatga aggggctcga ggagctgacg ggacgcgagg tgcggaccgt
     6301 cgtcctcggc cacctcctcc ggggcgggtc gccgtcgagc ttcgaccgcc tcctcgcgct
     6361 ccggttcggc gcggcggccg tgcgcgcgct cgagtcgggg cagagcggcg tcatggtcgc
     6421 cctggcgccg ccgacggtga actacgtccc gctcgag
//
LOCUS       DAOTEZ010000238         6408 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_8655_length_6408_cov_6.148276, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000238 DAOTEZ010000000
VERSION     DAOTEZ010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6408
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8655_length_6408_cov_6.148276"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="P5164_12675"
     CDS             complement(<1..603)
                     /locus_tag="P5164_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRY44789.1"
                     /translation="MGLLTKKSRAGADEPPHFPMHVLLVLATLYAIYPVLWVLSIAFS
                     GRQNLAIADVPPDPTFWDRLRTVVPWPAEVSLSNFRSLFADQPFATWVLNSAIVSIAT
                     TVVGVFLACTAAYAFSRFRFPGRRVGMMSFLVSQMFPGTLMLIPLYIILVKWLNLGST
                     RTGLVLAYSTTAIPFCVWMLKGYFDTIPRDLEEAALIDGAS"
     gene            complement(760..>1155)
                     /locus_tag="P5164_12680"
     CDS             complement(760..>1155)
                     /locus_tag="P5164_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY44790.1"
                     /translation="ATNGWLSFPFMMVISLGALQSIPADLYEAARVDGASRWQQFTAI
                     TLPSLKPALVPAIILSVVWTFNMFNIIYLVTAGQPAGSTEILITQSYKFAFQKYQYGY
                     AAAYSTVIFGILLVYGVFQNRMTKATEAI"
     assembly_gap    1156..1255
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1256..2936)
                     /locus_tag="P5164_12685"
     CDS             complement(<1256..2936)
                     /locus_tag="P5164_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY44791.1"
                     /translation="MSERPDETEKEPSHRLRFGAGLLVATLLVAGVGHRLLSTALGET
                     ARFGEDRRAVVSLRALTDLVARAGDGEAVRSVVAGFAAAQPRVAATRVVLFDGVSLEA
                     STDPADSGDDAAPRRLKREEKELYDRGQRLRAAIETNRAEGGARKDEVEKERLADGSL
                     SLAAPIEREGDVVGMVETRRVPPPPAPRPSLLTTLSYVVAPVVLFALLSLVLGDRRAP
                     LGALAAILLAGTAWGYAAQSSAKLSRARTALEADVAASVKESAAKAQELLAALPAPAA
                     PEAPADGAAPEAVPAPSPAPLDPAEWDSDAFRRPLGLSDGAGNVDAAKVAAAIASASA
                     TIRRSSLLLGAIALLLLGFVGLGGLAAFLRTLKTHRTAYAYVVPAMVGMVLLVFFPFV
                     YGIVLSFTDQTIYNSNAPLTEIWVGFKNYVSILTDFRVARTAADGALVFNYQNFYWTL
                     WVTIAWTVTNVTYGVVVGLILALILNTKGLALKPAYRVLLILPWAMPNYITALIWQGM
                     FHQQFGVVNQFVQMLGMEPVSWFETPATSFFTAWATNGWLSFPFMMVISLGA"
     gene            complement(2938..4095)
                     /locus_tag="P5164_12690"
     CDS             complement(2938..4095)
                     /locus_tag="P5164_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002620871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY44792.1"
                     /translation="MAQKEVVVWHAYRAEEKAALEKVVAAYNATVASKGIKVTTLAVP
                     YDAFADKITASVPRGKGPDIFIFAQDRLGGWIEAGKTVEPIDFFLDKATIARFIPTTM
                     EAMTYRGTVYGLPLNYKVITLIYNKKLLPTPPKTSKELVAVAKKLTDAKSGRFGLAYS
                     YSDFYYHAALQNAFGGRVFDPGPKPVLNAPENVKALELLMRWFLKDGILPAEPSTALI
                     TSLFNGGKAAMVFSGPWFLGEIADSIDYGLAPLPTIDEAGGKPMRPWMTVEGVYVTAP
                     SKNKDAAYDFAKYLTDTPQAKVMALEGRQTPSNKAVYSDPAVAGDKVLSAFRKQVEVA
                     VPMPNLPEMTMVWSPATTAMNTIVKRAATPKAAMDQAQAAVLKDVAALRKK"
     gene            complement(4178..5275)
                     /gene="ugpC"
                     /locus_tag="P5164_12695"
     CDS             complement(4178..5275)
                     /gene="ugpC"
                     /locus_tag="P5164_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRY44793.1"
                     /translation="MAEVVFQGVAKSYGDVKVIEGLDLEIRDQEFMVLVGPSGCGKST
                     ALRMIAGLEEISGGTVSIGGRVVNEVAPKDRDIAMVFQSYALYPHMTIRENLEFGLKI
                     RKLPQAEIDTLVNEAAEILGIAHLLDRKPKQLSGGQRQRVAVGRAITRKPAVFLFDEP
                     LSNLDAKLRVQMRAEISKLQNRLKTTTVYVTHDQVEAMTMGHRIAIMKDGKLQQVGTP
                     LEVYEQPANLFVAAFIGTPPMNFVKATFADGGGTLKAGTFSLPVPASLRGLTGAKDGK
                     AVVVGIRPENVVDPQKPTRGETARLGATVEVIEPLGHEVLVYARLGGDDVVVAKIDPH
                     RAPAYDDKIELVVELDALHLFDAATELRLTA"
     gene            complement(5291..>6408)
                     /locus_tag="P5164_12700"
     CDS             complement(5291..>6408)
                     /locus_tag="P5164_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRY44794.1"
                     /translation="PRFGSETDFIALVEACHARGIRVLLDVVYNHAGYESKYTKDPAT
                     KGWFRTNEKGDCGSDDVTSCVAGLPDFRTEDPEVAKYLLDAQIAWAKRLGVDGFRLDT
                     VKHVAHDFWTEHRRRTRAEVGKGFFLLGEVWGGDPQVLDPWFERDEMDAGFDFAFQGN
                     ALAFVEGRGRTVAFDRYLKSRERVRPGYLLSHFLSSHDVPGGLFQLGGNVELFRLAAV
                     LQMTTAGLPTIYYGEEVARPGGDWPDNRSDMPWGARDVKPGAGLTRNEALRADYVKLV
                     SIRKTFPALRRGTHEGLVTQGDAYVFLRRDAASGDAVAVAVNRAAEPVPVTFPAPAEW
                     EGATPKDLFGGLAVTFAEGSVSLTVPPRGAAILASGR"
BASE COUNT          966 a         2025 c         2377 g          940 t
ORIGIN      
        1 ggaggcgccg tcgatgaggg cggcctcctc gaggtcccgc gggatcgtgt cgaagtatcc
       61 cttcagcatc cagacgcaga aggggatcgc cgtcgtcgag taggcgagga cgaggcccgt
      121 ccgcgtcgac ccgaggttca gccacttgac gaggatgatg tagagcggga tgagcatcag
      181 cgtcccgggg aacatctgcg agacgaggaa cgacatcatc ccgacccggc gccccgggaa
      241 gcggaagcgc gagaacgcat aggccgccgt gcacgcgagg aagacgccga cgaccgtcgt
      301 cgcgatcgag acgatggcgc tgttgaggac ccacgtggcg aagggctggt ccgcgaagag
      361 cgaccggaag ttcgagagcg agacttcggc cggccagggg acgacggtgc ggagacggtc
      421 ccagaacgtc gggtcgggag gcacgtcggc gatggcgagg ttctgcctgc ccgagaaggc
      481 gatcgagagg acccagagga ccgggtagat cgcgtagagc gtggccagga cgaggaggac
      541 gtgcatcggg aagtggggcg gctcgtcggc gccggcgcgg gacttcttcg tcagcaggcc
      601 catgtcagat cgcctccgta gcgctcgccg ggggggctgc ctcgccgggg ggctgagctg
      661 cgagcgcccc ccggaccccc accacagtct tgtgtctccg cgatccgcga agcgcccgtc
      721 gaacgccccc ccggaccccc cgactcgaaa cagtgctcat cagatcgcct ccgtagcctt
      781 ggtcatgcgg ttctggaaga cgccgtagac gagcaggatt ccgaagatga ccgtcgagta
      841 ggccgcagcg tagccgtact ggtacttctg gaacgcgaac ttgtacgact gcgtgatcag
      901 gatctccgtc gagccggcgg gctggccggc ggtgacgagg tagatgatgt tgaacatgtt
      961 gaacgtccag acgaccgaca ggatgatcgc cggcacgagc gcgggcttca gcgacgggag
     1021 cgtgatcgcc gtgaactgct gccagcgcga ggcgccgtcc acacgggcgg cctcgtagag
     1081 gtcggccggg atcgactgga gggcgccgag ggagatgacc atcatgaacg ggaagctgag
     1141 ccacccgttc gtcgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngggcg
     1261 ccgagggaga tgaccatcat gaacgggaag ctgagccacc cgttcgtcgc ccacgccgtg
     1321 aagaacgacg tggccggggt ctcgaaccac gagaccggct ccatcccgag catctgcacg
     1381 aactggttca cgacgccgaa ctgctggtgg aacatcccct gccagatgag ggcggtgatg
     1441 tagttcggca tcgcccaggg gaggatgagg aggacccggt aggccggctt cagggcgagc
     1501 cccttcgtgt tcaggatcag cgcgaggatg agcccgacga cgacgccgta cgtcacgttc
     1561 gtcaccgtcc aggcgatcgt gacccagagc gtccagtaga agttctggta gttgaagacg
     1621 agggcgccgt cggcggccgt tcgggcgacc ctgaagtccg tcaggatgga gacgtagttc
     1681 ttgaagccga cccagatctc cgtcagcggc gcgttggagt tgtagatcgt ctggtcggtg
     1741 aacgagagga cgatgccgta gacgaagggg aagaagacga ggaggaccat cccgaccatg
     1801 gccgggacga cgtaggcgta ggcggtgcgg tgagtcttca gtgtccggag gaaggcggcg
     1861 agaccgccga ggccgacgaa cccgaggagg aggagcgcga tggctccgag gaggagcgac
     1921 gaccggcgga tcgtcgcgct cgcggaagcg atcgcggcgg cgaccttcgc cgcgtcgacg
     1981 ttccctgcgc cgtcggagag accgagcggg cggcggaagg cgtcggagtc ccactcggcg
     2041 gggtcgaggg gcgcggggga gggcgcaggg acggcctcgg gagcggcccc gtcggcgggc
     2101 gcctccggag cggccggcgc ggggagcgcg gcgaggagct cctgcgcctt cgcggcggac
     2161 tccttcaccg acgccgcgac gtcggcctcg agcgccgtcc gcgcgcggga gagcttcgcc
     2221 gacgactgcg ccgcgtatcc ccaggcggtc ccggccagga ggatcgcggc gagggcgccg
     2281 agcggggccc tccgatcgcc gaggacgagc gagaggaggg cgaagaggac gacgggggcg
     2341 acgacgtagg agagcgtcgt caggagcgaa gggcgtggcg ccggcggggg cgggacgcgc
     2401 cgggtctcga ccatcccgac gacgtcgccc tcgcgctcga tcggcgcggc gagagagagg
     2461 gagccgtcgg ccagacgttc cttctcgacc tcgtccttcc gcgcgccccc ctccgcccgg
     2521 ttcgtctcga tggcggcgcg gaggcgctgg ccgcggtcgt agagctcctt ctcctcccgc
     2581 ttcaggcgac gcggggccgc gtcgtcgccg gagtcggcgg ggtcggtcga ggcctcgagg
     2641 gagacgccgt cgaagaggac gacgcgcgtc gcggcgaccc ggggctgcgc ggccgcgaag
     2701 ccggcgacga ccgagcggac cgcctctccg tcgcccgccc gggcgacgag gtcggtcagc
     2761 gcgcgcaggg agacgacggc gcggcggtcc tcgccgaagc gggccgtctc gccgagcgcg
     2821 gtcgacagga gccggtggcc gacccccgcg acgaggagcg tggcgacgag gaggccggct
     2881 ccgaaccgga gccggtggga gggctccttc tcggtctcgt ccggccgctc gctcacgtca
     2941 cttcttcctc agggccgcga cgtccttcag cacggcggcc tgcgcctggt ccatcgccgc
     3001 cttcggcgtg gcggcccgct tgacgatggt gttcatcgcg gtcgtggcgg gcgaccagac
     3061 catcgtcatc tcggggaggt tcggcatcgg gacggcgacc tcgacctgct tgcggaacgc
     3121 ggagaggacc ttgtctcccg cgacggccgg gtcggagtag acggccttgt tcgagggggt
     3181 ctgacgtccc tcgagcgcca tcaccttggc ctgcggcgtg tcggtgaggt acttcgcgaa
     3241 gtcgtaggcg gcgtccttgt tcttcgacgg ggccgtcacg tagacgccct cgaccgtcat
     3301 ccaggggcgc atcggcttgc cgcccgcctc gtcgatcgtc gggagcggcg cgaggccgta
     3361 gtcgatgctg tcggcgatct cgccgaggaa ccaggggccg gagaagacca tcgccgcctt
     3421 cccgccgttg aagagcgacg tgatgagcgc ggtggacggc tccgcgggga ggattccgtc
     3481 cttcaggaac cacctcatga ggagctcgag cgccttcacg ttctcggggg cgttcaggac
     3541 cggcttcggg ccggggtcga agacgcgccc gccgaaggcg ttctggagcg ccgcgtggta
     3601 gtagaagtcg gagtaggagt aggcgaggcc gaagcggccc gacttcgcgt cggtcagctt
     3661 cttcgccacg gcgacgagct ccttcgacgt cttcggcggg gtcggcagca gcttcttgtt
     3721 gtagatgagc gtgatgacct tgtagttgag cggcaggccg tagaccgtgc cgcggtaggt
     3781 catcgcttcc atcgtcgtcg gaatgaagcg cgcgatcgtc gccttgtcga ggaagaagtc
     3841 gatcggctcg acggtcttgc ccgcctcgat ccagccgccc aggcggtcct gggcaaagat
     3901 gaagatgtcg ggtcccttcc cccgtggtac ggaggcggtg atcttgtcgg cgaaggcgtc
     3961 gtacgggacg gcgagcgtcg tgaccttgat tcccttcgaa gcgaccgtcg cgttgtaggc
     4021 agcgaccacc ttctcgaggg cggccttctc ctcggcccgg taggcgtgcc agacgacgac
     4081 ctccttctgg gccatgaggg aggtggccgg cgcggcgagg gcggcgaaga ggacggggag
     4141 cgcgaggcgg gagagcttca tgcgtgttcc tccgggttca ggccgtcaga cggagctccg
     4201 tcgcggcgtc gaagaggtgg agggcgtcga gctcgacgac gagctcgatc ttgtcgtcgt
     4261 aagccggagc gcgatggggg tcgatcttgg cgacgacgac gtcgtcgccg ccgagccgcg
     4321 cgtagacgag gacctcgtgg ccgagcggct cgatgacctc gaccgtcgcg ccgaggcggg
     4381 ccgtctcgcc gcgggtcggc ttctgcgggt cgacgacgtt ctcgggccgg atgccgacga
     4441 cgaccgcctt tccgtccttc gcgcccgtca ggccccggag cgaggccggg accgggagcg
     4501 agaacgtccc cgccttcagc gtcccgccgc cgtcggcgaa cgtcgccttg acgaagttca
     4561 tcggcggggt gccgatgaag gcggccacga agaggttcgc gggctgctcg tagacctcga
     4621 gcggcgtccc gacctgctgg agcttgccgt ccttcatgat cgcgatgcgg tggcccatcg
     4681 tcatcgcctc gacctggtcg tgggtgacgt agaccgtcgt cgtcttcagc cggttctgga
     4741 gcttgctgat ctcggcccgc atctggacgc ggagcttggc gtcgaggttc gagagcggct
     4801 cgtcgaagag gaagacggcg ggcttgcggg tgatggcgcg cccgaccgcg acgcgttgcc
     4861 gctgcccgcc cgagagctgc ttcggcttgc ggtcgaggag gtgggcgatg ccgaggatct
     4921 cggccgcctc gttcacgagc gtgtcgatct cggcctgggg gagcttgcgg atcttcaggc
     4981 cgaactcgag gttctcgcgg atcgtcatgt gcgggtagag cgcgtagctc tggaagacca
     5041 tcgcgatgtc gcggtccttc ggggcgacct cgttcacgac gcggccgccg atcgagaccg
     5101 tcccgccgga gatctcctcg agccccgcga tcatccggag ggcggtcgac ttgccgcagc
     5161 ccgagggccc gacgaggacc atgaactcct ggtcgcggat ctcgaggtcc agcccctcga
     5221 tgactttgac gtcgccgtag ctcttcgcga cgccctggaa gacgacttcg gccatgcgcg
     5281 ggcctcctgc tcagcgcccg gacgccagga tcgcggcgcc ccggggcgga acggtcagag
     5341 agacggagcc ctccgcgaac gtgacggcga ggccgccgaa gaggtccttc ggcgtcgcgc
     5401 cctcccattc cgcgggggcg gggaaggtga ccgggacggg ctcggcggcg cggttgacgg
     5461 cgacggcgac ggcgtcgccc gacgcggcgt cgcggcggag gaagacgtag gcgtccccct
     5521 gcgtgacgag cccttcgtgc gtcccgcgcc ggagcgcggg aaaggtcttc cggatcgaga
     5581 cgagcttcac gtagtcggcc cggagcgcct cgttccgcgt caggccggcg ccgggcttca
     5641 cgtcccgcgc gccccacggc atgtcgctcc ggttgtcggg ccagtcgccg ccggggcggg
     5701 cgacctcctc gccgtagtag atcgtcggga ggccggccgt cgtcatctgg aggaccgccg
     5761 cgaggcggaa gagctccacg ttcccgccca gctggaagag cccgcccggg acgtcgtgcg
     5821 aggagaggaa gtgggagagg aggtagccgg ggcggaccct ctcgcgcgac ttgaggtagc
     5881 ggtcgaaggc gaccgtccgc ccgcgcccct ccacgaaggc gagggcgttc ccctggaacg
     5941 cgaagtcgaa gccggcgtcc atctcgtccc gctcgaacca ggggtcgagg acctgcgggt
     6001 cgccccccca gacctcgccg aggaggaaga accccttccc gacctccgcg cgcgtccgcc
     6061 gccggtgctc ggtccagaag tcgtgggcga cgtgcttgac ggtgtcgagg cggaagccgt
     6121 cgacgccgag ccgcttcgcc caggcgatct gggcgtcgag gaggtacttc gcgacctcgg
     6181 ggtcctcggt ccggaagtcg gggaggccgg cgacgcacga ggtgacgtcg tcgctcccgc
     6241 agtccccctt ctcgttcgtc cggaaccagc ccttcgtcgc cgggtccttc gtgtacttcg
     6301 actcgtaccc cgcgtggttg tagacgacgt cgaggaggac ccggatgccg cgtgcgtggc
     6361 acgcctccac gagcgcgatg aaatcggttt cagagccgaa gcgcgggt
//
LOCUS       DAOTEZ010000239         6399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_8671_length_6399_cov_6.923707, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000239 DAOTEZ010000000
VERSION     DAOTEZ010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6399
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8671_length_6399_cov_6.923707"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            510..2312
                     /locus_tag="P5164_12705"
     CDS             510..2312
                     /locus_tag="P5164_12705"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY44795.1"
                     /translation="MRILARKERVVLGVAIAALAANAVLTVGHLDRLVEGHREASRSG
                     LILTELARVEATLGAAEAAQRRFLLARGATSLAPLEAARTVLAIQLDRLAELTQGDVR
                     QGDRVAQLSLAARRRLVLLNDGVRRYRQAGTTDLPQEDPAEREAAAAIRSALDALEGA
                     ERRTLAAQEAATARSRLAAGAAAFASTILGFCLVVVGLLLLRRELAADAAATAAVEKA
                     NAALRDADRRKDEFLASLAHELRNPLAAMRNSVELLDASEGGDDQKARERKGLARAVL
                     RRQLAHLTRLVDDLLDLSRVASSRLVLKKEAVTLSEIIEAALETTRPALEARRHELSV
                     ASPRENVLVEGDPVRLAQVVSNLLSNAAKYSPPGGRIELAVAADEVEAIVRVTDDGIG
                     LAPRDLERVFGLFEQGSARPVDDEGLGVGLALSRRLAQLHGGSLTATSAGPGHGSTFT
                     LRIPRASAPEREAAPLATAPAPASTTACRVLLVEDNADGARSLAELLSLAGHEVHVAS
                     DGPSGLEAARRLLPDAVVLDVGLPGFDGYELARRLRAESSLAGVLLIALSGWAGRDDR
                     ARSSDAGIDHHLVKPAGVARLGELLATVTRRPGT"
     gene            complement(2290..3636)
                     /locus_tag="P5164_12710"
     CDS             complement(2290..3636)
                     /locus_tag="P5164_12710"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRY44796.1"
                     /translation="MKALVVEDDPGARRALAELLVELGHEPAEVGSVADALASLEGDP
                     PDVAIVDLGLPDGDGLEVVRAAPGATAVLVLTGQGSVRNAVEAMKAGAHDFLVKPLRL
                     PQLRAALDLLDGRGARPPVGASRGGVDALGELLGGSPPMKEVLRLLARVAPSDAPVFL
                     WGESGTGKEVAARTLHSLSRRAAGPFVGVNCGAISPNLVESELFGHEKGAFTGAERRR
                     EGTFELARRGTLFLDEVTEMPLEMQVKLLRVLETKSFRRVGGTEERDVDVRVVASTNR
                     DVLEAVASGVLREDLYYRLAVFPLRLPPLRERLADVPVLALEFLRRVEAEEGRGIRDI
                     EPEALAHLGRHAWPGNVRELRNAIHRAYVLSDPPAISGEAAEAVLAAPAPSLPGGASG
                     EALAVPVRVGDTARVAERKLLEATLAAVGGDKRAAAEMLGLSLKTVYNKLRSRDVS"
     gene            complement(3633..4229)
                     /locus_tag="P5164_12715"
     CDS             complement(3633..4229)
                     /locus_tag="P5164_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44797.1"
                     /translation="MPVSPSVLAVLRHALADPLSAATVKVEVLATRLDREAPSLSPRA
                     REVGDDLASAGRLLDLLAAVAAVAEEAPEETSVASLVEGLDGGVAAGESAHLLRVRPS
                     AAGLALRRLLAFGAARGAAPRVEVHASPAGAELLVAPLGPAPAAPEKLLLLPRDVADA
                     DELFLARVAVEADGGSLRLVERGGALEARLFWPREEER"
     gene            4468..5424
                     /locus_tag="P5164_12720"
     CDS             4468..5424
                     /locus_tag="P5164_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44798.1"
                     /translation="MRIARNSNDTRPMLWTLAPLLGVALAFPACSKTDVGSQPKASAE
                     ALTEQNRLLLAENEKLAERVRTAEAADAEAAATLAEVQASLEEIRGKELKILRKTLDV
                     TREGQARTSTREMLADELATIRSAIRKNLDRIASLERERKADGRKMASLQTLVDELKR
                     SLEEKSVTIATLEQTVFELNGRVREQEGVILAKDTLIREKEDVIATRTQEANTAFVAV
                     ADKKTLKEKGVVEKKGSILGLGGAWQETGKYDPQVFRAIDTTVEAELTIPAPAEKVRV
                     LPGHPEESYQIVSGGPNSSTLKVTDREAFWRDSRYLVVMIPN"
     gene            complement(5595..>6399)
                     /locus_tag="P5164_12725"
     CDS             complement(5595..>6399)
                     /locus_tag="P5164_12725"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423090.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRY44799.1"
                     /translation="AACNVKKEGVEVKPEIMIPLVGHVNELKDQEKLVREVAKDVFAK
                     AGIKVDYLVGTMIELPRAALTADEIATVAEFFSYGTNDLTQTTFGLSRDDAGKFLPDY
                     FRREILPADPFERIDQAGVGKLVKMGVELGRTTRPKLKIGICGEHGGEPTSVEFCHRT
                     NLDYVSCSPFRIPIARLAAGQAAVREGATVSGRPAKAAAKAAAKPTPKKAAPKAKPAP
                     KAKVPAKKAARVKAPAKVAKKVAKPAPKRAAKPAPRKPAKKAPAKKGRK"
BASE COUNT          860 a         2425 c         2264 g          850 t
ORIGIN      
        1 tcgacgaccc ggtcgcctgg cacgagccgg gctcgccgcc cgctccgcgc tgagcccgtc
       61 ggaacagctg cacggcgcgg ccgggacgac ggggaggatt gaagagggta cgcggcgccc
      121 ggggagggcg ccgccgcgtc ggggaaaagc gggaaggacc tcggggaccc gcaggcgacg
      181 gtccggcgcc gctcctgcgg gcctcatccg atctcgcctg ctggctttgt gggcctcgtc
      241 ccgggggggt tccggtaccg aggcccctcc cactgtcgct ttcgtgtcgg atcgggttcg
      301 tccgcctcga ccacctcctc gagggctcac ggccctgcac ccatcggagc aaaccttctg
      361 ccagtcgatg gaggacgcgc ccgcgcctcg cctcgcaacc gcgcggacag ccgccctgcg
      421 ggcctcgttg gccgcgagag agcgcgcttc cgtcgacgct gctcgtccga gaacgttgaa
      481 attcctgccg tccgggagaa gatctttcca tgcgcatcct cgcgcgcaag gaacgggtcg
      541 tcctcggcgt ggccatcgcc gcgctcgccg cgaacgccgt cctgacggtc gggcacctcg
      601 atcgcctcgt cgagggacac cgggaggcga gccggtccgg tctgatcctg acggagctgg
      661 cgcgggtcga agcgacgctc ggcgcggccg aggccgccca gcggcggttc ctcctggcgc
      721 ggggcgcgac gtcgctcgcc ccgctcgagg cggcgcggac ggtcctcgcc atccagctcg
      781 accggctcgc agagctgaca cagggcgacg tgcgacaggg cgaccgcgtc gcgcagctct
      841 ccctcgcggc gcgtcggcgg ctcgtcctcc tcaacgacgg ggtgcgccgt taccggcagg
      901 ccgggacgac ggatctcccg caggaggacc cggcggagcg cgaggcggcg gccgccatcc
      961 gctccgcact cgacgcgctc gagggcgccg agcggcgaac gctcgcggcg caggaggccg
     1021 cgacggcgcg gagccggctc gccgccggcg ccgccgcgtt cgcctccacg atcctcggct
     1081 tctgcctcgt cgtcgtcggc ctcctcctcc tacggcgcga gctcgccgcc gacgccgccg
     1141 cgacggccgc cgtcgagaag gcgaacgccg ccctgcgcga cgccgaccgg cgaaaggacg
     1201 agttccttgc ctccctcgcg cacgagctgc ggaaccctct cgccgcgatg aggaattccg
     1261 tggagctcct cgacgcctcg gagggcggcg acgaccagaa ggcgcgggag cggaagggcc
     1321 tcgcgcgcgc cgtcctccgg cgccagctcg cgcacctgac gcgcctcgtc gacgatctcc
     1381 tcgacctctc gcgcgtcgcg agcagccggc tcgtcctcaa gaaggaggcc gtcaccctct
     1441 ccgagatcat cgaggctgcg ctcgagacga cgcgccccgc gctcgaggcc cggcggcacg
     1501 agctctccgt cgcctcgccg cgcgagaacg tcctcgtcga aggggacccg gtccgcctcg
     1561 cacaggtcgt ctcgaacctc ctctcgaacg ccgcgaagta ctcacccccg ggcggtcgga
     1621 tcgagctcgc cgtcgccgcc gacgaggtcg aggccatcgt gagagtgacc gacgacggga
     1681 tcgggctggc gccccgggac ctggagcggg tcttcggcct cttcgagcag ggctccgccc
     1741 gtccggtcga cgacgagggg ctcggcgtcg gcctcgcgct ctcgcgccgc ctcgcgcagc
     1801 tccacggcgg gagcctgacc gcgacgagcg ccgggccggg ccacggcagc acgttcacgc
     1861 tccggatccc gcgggcgtcc gcgcccgagc gcgaggccgc acccctggcc acggccccgg
     1921 ccccggcctc gacgacggcc tgccgggttc tcctcgtcga ggacaacgcc gacggggccc
     1981 ggtcgctcgc cgagctcctg tctcttgccg gccacgaggt ccacgtcgcc tccgacgggc
     2041 cgtccgggct cgaggccgcc cgccgcctcc ttccggacgc cgtcgttctc gacgtcggcc
     2101 tgccgggctt cgacggctac gagctcgcgc gccgtcttcg ggcggagtcc tccctcgcgg
     2161 gcgtcctcct gatcgcgctc tcgggctggg ccgggcgcga cgaccgggcc cggtcgtcgg
     2221 acgcggggat cgaccaccac ctcgtcaagc ccgcgggcgt cgcccgcctc ggcgagctcc
     2281 tcgcgacggt cacgagacgt cccgggacct gagcttgttg tagacggtct tcagcgacag
     2341 cccgagcatc tcggccgccg ctctcttgtc ccccccgacg gcggcgagcg tcgcctcgag
     2401 gagcttgcgt tcggcgaccc gcgccgtgtc gccgacccgc accgggacgg cgagcgcctc
     2461 ccccgacgcc ccaccgggga gcgacggcgc cggcgcggcg aggacggcct ccgcggcctc
     2521 gccggagatc gcgggcgggt cggagagcac gtacgcgcgg tggatcgcgt tccgcagctc
     2581 gcggacgttc ccgggccagg cgtggcggcc gagatgggcg agcgcctccg gctcgatgtc
     2641 cctgatcccg cgcccctctt ccgcctcgac gcgccggagg aactcgagcg cgaggacggg
     2701 gacgtccgcg agccgctcgc ggagcggcgg gagacggagg gggaagaccg cgagccggta
     2761 gtagaggtcc tcccggagaa cgccgctcgc gacggcctcc aggacgtcgc ggttcgtgct
     2821 cgccacgacg cggacgtcca cgtcgcgctc ctccgtccct ccgacgcgcc ggaacgactt
     2881 cgtctcgagg acccgcagga gcttcacctg catctccagc ggcatctcgg tcacctcgtc
     2941 gaggaagagc gtgccgcggc gggccagctc gaacgtccct tccctcctcc gctcggcccc
     3001 ggtgaaggcc cccttctcgt gcccgaagag ctcgctctcg acgaggttcg gcgagatggc
     3061 gccgcagttc actccgacga agggcccggc cgcgcgccgc gagagggagt gcagcgtccg
     3121 cgcagccact tcctttcccg tgccactctc gccccagaga aagaccgggg cgtcggacgg
     3181 ggcgacgcgc gcgaggagcc ggaggacctc cttcatcggg ggcgatccgc cgaggagctc
     3241 gccgagcgcg tcgactccgc cccgagatgc cccgacgggc gggcgagcgc cccgcccgtc
     3301 gaggagatcg agcgccgcgc gcagctgcgg caggcgcagc ggcttcacga ggaagtcgtg
     3361 cgcgccggcc ttcatcgcct cgacggcatt cctgacggac ccctggcccg tcaggacgag
     3421 gacggccgtc gcgccgggag cggcgcgcac gacctcgagc ccgtccccgt ccgggaggcc
     3481 gaggtcgacg atcgcgacgt ccggcgggtc cccctcgagc gacgcgaggg cgtcggccac
     3541 gctcccgacc tccgccggct cgtgccccag ctcgacgagc agctcggcga gcgcccggcg
     3601 cgccccggga tcgtcctcga cgacgagcgc cttcatcgtt cttcctcgcg cggccagaag
     3661 agcctcgcct cgagcgcgcc gccgcgttcc acaagccgga gcgagccccc gtccgcctcc
     3721 acggccaccc gcgcgaggaa gagctcgtcg gcgtccgcga cgtcccgcgg caggagaagc
     3781 agcttctccg gagccgccgg cgccgggccg agcggcgcga cgaggagctc cgctccggcc
     3841 ggagaggcgt gaacctcgac gcgcggcgcg gccccccgcg cggcgccgaa ggcgaggagc
     3901 cgccggaggg cgaggccggc ggcggacggc cggacgcgca ggaggtgagc ggactctccc
     3961 gcggcaaccc cgccgtcgag cccctcgacg agggacgcga cggacgtctc ctccggcgcc
     4021 tcttcggcga ccgccgcgac cgcggcgagg agatcgagga gccgccccgc cgacgcgagg
     4081 tcgtcgccga cctcgcgcgc cctcggcgag agagaaggcg cctcgcggtc gagccgtgtc
     4141 gcgaggacct cgaccttcac cgtcgccgcc gagagagggt cggcgagggc gtgacgcagg
     4201 accgcgagca cggacgggga gacgggcatg ccccaacgct accagcgtgt cccgcgtttc
     4261 gacaccccgg aggcccgccg gagcccgcgg tgtccgcttc ttacacgttc gttgtaatca
     4321 cttccgtccg gccgaccagg cccgctattc cgggcgtgag gtgcgcgacg acggcaactt
     4381 cttcgctcct catgccttgc gctgtcccgg ggagaggttc cggcgctggc gcggcgcttg
     4441 aaagcacgct cggcacggag gtcaaccatg agaatcgcga ggaactcgaa cgacacgcgc
     4501 cccatgctct ggactctcgc cccgcttctc ggcgtcgcgc tcgccttccc ggcctgctcg
     4561 aagacggatg tcggcagcca gccgaaggcg agcgcggaag cgctcacgga gcagaatcgc
     4621 ctcctcctcg ccgagaacga gaagctcgcc gagcgggtcc ggacggcgga ggccgcggac
     4681 gccgaggcgg ccgccaccct cgccgaggtg caggcgagcc tcgaggagat ccgcggcaag
     4741 gagctgaaga tcctcaggaa gaccctcgac gtcacgcgcg agggacaggc ccggacgagc
     4801 acccgggaga tgctcgccga cgagctcgcg acgattcgga gcgcgatccg gaagaacctc
     4861 gaccggatcg cgtcgctcga gcgtgagcgg aaggcggacg gccggaagat ggcctcgctc
     4921 cagaccctcg tcgacgagct gaagcgctcg ctcgaggaga agagcgtcac catcgcgacg
     4981 ctcgagcaga ccgtcttcga gctgaacggc cgggtgaggg agcaggaggg cgtcatcctg
     5041 gcgaaggaca cgcttatccg cgagaaggag gacgtcatcg cgacccggac gcaggaggcc
     5101 aacaccgcgt tcgtcgcggt cgccgacaag aagacgctca aggagaaggg cgtcgtcgag
     5161 aagaagggct cgatcctcgg cctcggcggc gcctggcagg agaccgggaa gtacgacccg
     5221 caggtcttcc gcgcgatcga caccacggtg gaggcggagc tcacgatccc ggcgcccgcg
     5281 gagaaggtcc gagtccttcc cgggcatccg gaggagagct atcagatcgt ctccggaggg
     5341 ccgaactcgt cgacgctgaa ggtcacggat cgcgaagcct tctggcgcga cagccggtac
     5401 ctcgtcgtca tgatcccgaa ctgacccggc tctcccggtt cctgcacccc gcccgcggcg
     5461 gccgaccggc cgccgcgggc cacttctcga cgcagccccc ctggaaaacg acggcggccc
     5521 ttcggccgcc gtcgtcggtt tccgtttccg gggcggggcg cctcgcgccg gccctgccgg
     5581 cgcgagactc ggggctactt ccggcccttc ttcgccgggg ccttcttcgc gggcttcctc
     5641 ggggcgggct tcgcggccct cttcggcgca ggcttcgcga ccttcttcgc aaccttcgcc
     5701 ggagccttca ccctggcggc cttcttcgcg gggaccttcg ccttcggagc gggcttcgcc
     5761 ttcggagccg ccttcttcgg cgtgggcttc gccgcggcct tcgccgccgc cttcgcgggc
     5821 cggccggaaa ccgtcgcgcc ctcgcggacg gcggcctggc cggcggccag gcgggcgatc
     5881 gggatccgga acggcgagca gctgacgtag tcgaggttcg tccggtggca gaactccacc
     5941 gacgtcggct cgccgccgtg ctcgccgcag atgccgatct tcagcttcgg ccgggtcgtc
     6001 cggccgagct cgacgcccat cttcacgagc ttcccgacgc cggcctggtc gatcctctcg
     6061 aacgggtcgg cggggaggat ctcgcgccgg aagtagtcgg gcaggaactt cccggcgtcg
     6121 tcgcggctga ggccgaacgt cgtctgcgtc aggtcgttcg tgccgtagct gaagaactcg
     6181 gccacggtgg cgatctcgtc ggcggtcagc gccgcgcgcg gcagctcgat catcgtcccg
     6241 acgaggtagt ccaccttgat cccggccttc gcgaagacgt ccttcgcgac ctcgcggacg
     6301 agcttctcct ggtccttcag ctcgttcacg tggccgacga gcgggatcat gatctccggc
     6361 ttgacctcga ccccttcctt cttcacgttg caggccgcc
//
LOCUS       DAOTEZ010000240         6371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_8725_length_6371_cov_6.209151, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000240 DAOTEZ010000000
VERSION     DAOTEZ010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6371
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8725_length_6371_cov_6.209151"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="P5164_12730"
     CDS             <1..867
                     /locus_tag="P5164_12730"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY44800.1"
                     /translation="IGSGAVGSEFASCYARYGAKTVLVEVLPRLLPVEDEEVSKEVEK
                     SFRKKGIECWTGTAVEAVAENPDGTLKVAAKTSAGESKSWDVEWVILAVGRRPVTEGL
                     GLEAAGVGTERGYVRVDAFQRTNVPNVYAIGDCTPTIWLAHVASAEAIVAAETIAGHR
                     TEPINRDQVPGCTYCDPEVASIGLTEAKAKERGFDVKVGRFGFQVLAKSAMEHTNEGF
                     VKIVSDRKYDEVLGVHIVGPHATELIGQAAAFLRCEATTEEMVRTIHAHPTLSEALHE
                     AAEAVDGHNIHG"
     gene            943..2148
                     /locus_tag="P5164_12735"
     CDS             943..2148
                     /locus_tag="P5164_12735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY44801.1"
                     /translation="MRVLQLSPKVPWPPEDGGRVASRVLALSLLRAGAEVRLISLNPA
                     KHRVEAAALPDEARAVRLERVDHDTSLSVSGFLGSLLRGTSYNVDRFRSAAFERRVVA
                     ALAEERPDIVLLESLYLLPCLPALRRATRAPVVLRSFNVEHEIWDRLAPGERNPARRT
                     LLAHLARRLRAYEVASLDEPDAIVPVTAEDAETYRRLGATVPIHVAPVGLETALVPDR
                     SGQGERETLVFLGALDWRPNLEGVRWFLERVWPAIRRSVPGARIVVGGSNPPSGFEKR
                     LSGEGVTFSGRVPDAQAFFASGAAMVVPLLSGGGMRVKILEAMALGVPIVSTRLGATG
                     IAARDGVEIRLADEPDAMAAACVEVLSDPKGALRLGRAGRDCVLARFDADVIARDLLG
                     FLVDLLRRG"
     gene            2332..3288
                     /locus_tag="P5164_12740"
     CDS             2332..3288
                     /locus_tag="P5164_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015345872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HRY44802.1"
                     /translation="MKAKLIATALTLPFALTLAAIPPGERPDDRHASDTFVIGGEEFA
                     SQQDFIQAGRRCQTFHPDEETMAALEAEFLQELSLRPHSDFESLFTSPTYVNVYVHVI
                     NKGTGIANGDIPQSMIDDQMDVLNAAYSSTGVRFQLISVDRTTNSTWYTMSPGSTAES
                     QAKAALRKGSADDLNLYTANPGGGLLGWATFPSSYKSAPSKDGVVVLFSSLPGGSAAP
                     YNEGDTGTHEVGHWMGLYHTFQGGCKTGSNGGDYVSDTNAEKSAAYGCPTGRDSCAGS
                     RYPGLDPIENFMDYTDDYCMTLFTAGQVARMRAQWDTYRAGK"
     gene            3437..4612
                     /locus_tag="P5164_12745"
     CDS             3437..4612
                     /locus_tag="P5164_12745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY44803.1"
                     /translation="MSASIRKSRVLLAGAALPVAAILFLGLRAALGPAVDVAKVSRQD
                     LLETLVVSGRVLERSKASLGSPVTGTVDEVLFEEGDRVRGGQLLARLDEGEALASLAE
                     ARARLEQVSGGERRIAGEELRQAALRLEQAERELERLSALRRDGFVSVRDEDDARSAR
                     DLAASAVASSRERARSLAAGGADERAARAAVASAAARLEQRRVVAPGPGVVLVRSAEP
                     GDVVPAGKVLLTLALDRETWLLAQPDEKNLPSLRVGQKARATADAFPERAFDAEVTSI
                     APGVDLARGTVDVKLRVPDPPDFLRSDMTLSIELQVGARERALVVPLECVRDAAAEPW
                     ALAVRKGRAVRTPLVLGARGGKVAEVLQGLDEGELVVREPGRVEEGRRVRTRLASGG"
     gene            4614..5834
                     /locus_tag="P5164_12750"
     CDS             4614..5834
                     /locus_tag="P5164_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY44804.1"
                     /translation="MPFEVLVALRFLREGRIQTLLILSGIGVGVGVIVFLSALISGLQ
                     ASLIERTLGTQAHVVVRAPEDVVRPATAPTAERVVASRLEQPAQRLRSIVGWPQTLGL
                     LRSLPGIRAASPTAEGPAVALRGLANRSIALRGVEPASYRGIIDMPPRMTDGEFRVDA
                     AHAVLGVELAKDLGLRKGDKLRIETPRGRSDVFVVAGLFDVGNKDLNERWVFVSLRSA
                     QTLLDLSGGISSIELKVDELFAADRIAADIAGRTGLEAESWMERNRQLLVGLRSQSAS
                     SGMIQLFVVIAVALGIASVLVVSVVQKSREIGILKAMGTRTAQVVRVFLVQGAVLGLA
                     GSAFGILLGSALALFFAALATNPDGSPTFPVALTPGLFLGAAAVATVTGLVAAVAPAE
                     RAASLDPADVIRHG"
     gene            5827..>6371
                     /locus_tag="P5164_12755"
     CDS             5827..>6371
                     /locus_tag="P5164_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY44805.1"
                     /translation="MAEPLVLVEGVTKSFGREVVTKVLHGVDLELHPAEFTALVGPSG
                     SGKSTLLNLLGLLDRPTSGRIVLEGRDTTDLAEAERTTLRGRSLGFVFQFHHLLPAFT
                     ALENVMMPMLAARGREEPGMRERAFALLEEIGLADRASYGASKLSGGQQQRVAVARAL
                     VLDPPLVLADEPTGNLDTESGA"
BASE COUNT          880 a         2403 c         2153 g          935 t
ORIGIN      
        1 atcggctcgg gcgccgtcgg ctccgagttc gcgtcgtgct acgcgcgcta cggcgcgaag
       61 accgtcctcg tcgaggtcct tccccgcctc ctccccgtcg aggacgagga ggtctcgaag
      121 gaagtcgaga agtcgttccg gaagaaggga atcgagtgct ggaccgggac ggccgtcgag
      181 gccgtcgccg agaacccgga cggcacgctc aaggtcgccg cgaagacctc ggccggcgag
      241 tcgaagagct gggacgtcga gtgggtcatc ctcgccgtcg ggcggcgtcc cgtcaccgag
      301 gggctcggcc tcgaggcggc gggcgtcggg accgagcgcg gctacgtgag ggtcgacgcc
      361 ttccagcgga cgaacgtccc gaacgtctac gcgatcggcg actgcacccc cacgatctgg
      421 ctcgcccacg tcgcctccgc cgaggcgatc gtcgccgccg agacgatcgc cgggcaccgg
      481 accgagccga tcaaccgcga ccaggtgccg ggctgcacct actgcgaccc ggaggtcgcc
      541 tccatcggcc tgaccgaagc gaaggcgaag gagcgcggct tcgacgtgaa ggtcgggagg
      601 ttcggcttcc aggtcctcgc caagtccgcg atggagcaca ccaacgaggg cttcgtgaag
      661 atcgtctccg accggaagta cgacgaggtc ctcggcgtcc acatcgtcgg cccgcacgcc
      721 accgagctga tcggacaggc cgcggcgttc ctccggtgcg aggcgacgac cgaggagatg
      781 gtccggacga tccacgctca cccgacgctt tcggaagcgc tccacgaggc ggccgaagcg
      841 gtcgacgggc acaacatcca cggctgagac ggcactcccc cgtcgtgacg tccgcgtgcg
      901 gcctgccggc cgcgcgtgga cgcgcgccgg gaccgaggcg cgatgagggt cctccagctc
      961 tcccccaagg ttccctggcc tccggaggac gggggacgcg tcgcgtcgcg ggtgctcgcg
     1021 ctgtcgctcc tccgcgccgg ggccgaggtc cggctgatct ccctgaatcc ggcgaagcac
     1081 cgcgtcgagg ccgcggccct tccggacgaa gcgcgcgccg tccggctcga aagggtcgac
     1141 cacgatacgt cgctctccgt ctccggcttt ctcgggagcc ttctccgcgg gacctcctac
     1201 aacgtcgacc gattccgctc cgccgcgttc gagcgacgcg tcgtcgcggc gctggccgaa
     1261 gagcggccgg atatcgtcct cctcgagagc ctctatctcc tcccctgcct cccggctctg
     1321 cgccgcgcga cccgggcgcc cgtcgtcctt cgctctttca acgtcgagca cgagatctgg
     1381 gaccggctcg cgccgggaga gcgcaacccc gcccgtcgga ccctgctcgc gcacctcgcc
     1441 cggcgcctcc gcgcgtacga ggtcgcctcg ctcgacgagc ccgacgcgat cgtcccggtc
     1501 acggccgaag acgccgagac gtatcgccgg ctcggagcca cggtccccat ccacgtcgcc
     1561 ccggtcggcc tcgagacggc cctcgtcccg gaccgatccg gacagggcga gcgcgagacg
     1621 ctcgtcttcc tcggcgccct cgactggcga ccgaacctcg aaggcgtgcg ttggttcctc
     1681 gagcgtgtct ggcctgccat tcggagatcc gtccccgggg cgcggatcgt cgtcgggggc
     1741 agcaaccctc cctcgggctt cgagaagcga ctctccgggg agggagtgac gttctctggc
     1801 cgcgtcccgg acgcgcaggc gttcttcgcc tcgggcgcgg cgatggtcgt acccctcctc
     1861 tccgggggtg ggatgcgcgt caagatcctc gaggcgatgg ccctcggcgt tccgatcgtc
     1921 tcgacccgcc tcggagcgac cgggatcgcg gcacgggacg gcgtggagat tcggctggcc
     1981 gatgagccgg acgcgatggc agccgcctgc gtcgaggtcc tctccgaccc gaagggcgcc
     2041 ctgcgcctcg ggcgggcggg acgcgattgc gtgctcgccc gcttcgacgc cgacgtcatc
     2101 gcacgcgacc tcctcgggtt cctcgtcgac ctcctacgac gcggatagcc gggtcgtcct
     2161 gatcgacacc ttcggatccg gttctgaccc ggggtcaacg acagacccgc gtcgcagcag
     2221 aagcaggccc agaaagtgac ccctttcctg cgattcagtc ttcaaacgag atggcacggt
     2281 tctggctgtt gaggagcgac cgggcatccg gccatcgagg aggagagaac gttgaaagcg
     2341 aagctgatcg cgaccgccct gacgctcccg ttcgcgctga ccctggcggc gatcccgccg
     2401 ggtgaacgac cggacgaccg ccacgccagc gacaccttcg tcatcggcgg cgaagagttt
     2461 gccagccagc aggacttcat ccaggccggc cggcgctgcc agacgttcca ccccgacgag
     2521 gagacgatgg cggccctcga ggcagagttc cttcaggagc tcagcctccg cccccactcc
     2581 gacttcgagt cgcttttcac gagcccgacc tacgtcaacg tctacgttca cgtcatcaac
     2641 aaggggaccg gaatcgccaa cggcgacatc ccccagtcga tgatcgacga tcagatggac
     2701 gtcctgaacg cggcctactc ctcgacgggc gtccgcttcc agctcatctc cgtcgaccgg
     2761 acgacgaact ccacctggta cacgatgtcg ccgggctcca ctgccgaatc ccaggccaag
     2821 gcggccctcc gcaagggctc cgccgacgac ctgaacctct acaccgcgaa ccccggcggc
     2881 gggctcctcg gctgggcgac gtttccctcg agctacaaga gcgccccgag caaggacggc
     2941 gtcgtcgtct tgttctcctc gctccccggc ggctccgcgg ccccgtacaa cgagggcgac
     3001 accgggacgc acgaggtcgg gcactggatg ggcctctacc acacgttcca gggcggctgt
     3061 aagaccggct cgaacggggg cgactacgtc tccgacacga acgccgagaa gagcgccgcc
     3121 tacggctgcc cgacggggcg cgattcctgc gccggctcgc gctaccccgg cctcgacccg
     3181 atcgagaact tcatggacta caccgacgac tactgcatga cgcttttcac cgccgggcag
     3241 gtcgctcgca tgcgcgccca gtgggacacc taccgagcgg gcaagtaatc cgaccgacgg
     3301 cttcgacctg gagaggggag cggctttcgg gccgctcccc tttctgcctt ctccccgaga
     3361 tccgtctccg agccggacac ggaggagccg agggacggac gcaccgaccg cggctcgccg
     3421 tgccagactc gacgtgatga gcgcttcgat tcgaaagtcg cgcgtgcttc tcgccggggc
     3481 cgccctcccg gtggccgcga ttctcttcct gggcctgcgc gcggctctcg gacccgccgt
     3541 cgacgtggcg aaggtctcgc gacaggacct cctcgagacg ctcgtcgtga gcggccgcgt
     3601 cctcgagcgc tcgaaggcct cgctcggctc ccccgtcacc gggacggtcg acgaggtcct
     3661 cttcgaggaa ggggaccggg tccgcggcgg ccagctcctc gcccggctcg acgagggcga
     3721 ggcgctcgct tccctcgccg aggcgcgtgc ccggctcgag caggtcagcg gcggcgaacg
     3781 gcggatcgcc ggcgaggagc tccggcaggc agcgctccgc ctcgagcagg cggagcgcga
     3841 gctcgagcgc ctctccgccc tccggcgcga cggcttcgtg agcgtccgcg acgaggacga
     3901 cgcccggagc gcgagggacc tcgcggcgag cgccgtcgcc tcctcccgcg aaagagctcg
     3961 gtctctcgcc gcaggcggcg ccgacgagcg cgcggcccgc gccgccgtcg cttccgccgc
     4021 cgcacggctc gagcagcgcc gggtcgtcgc gccgggcccg ggcgtcgtgc tcgtccggtc
     4081 ggcggagccc ggagacgtcg tcccggccgg gaaagtcctc ctgacgctcg cgctcgatcg
     4141 cgagacgtgg cttctcgccc agcccgacga gaagaacctc ccttccctgc gagtcggcca
     4201 gaaggcgcgc gcgaccgccg acgcctttcc cgagcgcgcc ttcgacgccg aggtgacctc
     4261 gatcgccccc ggagtcgacc tcgcacgcgg aacggtcgac gtgaagctcc gcgtccccga
     4321 cccgcccgac tttctgcgga gcgacatgac tctctcgatc gagctccagg tcggcgcccg
     4381 cgagcgagcc ctcgtcgtcc cgctcgagtg cgtccgggac gccgctgccg agccgtgggc
     4441 gctcgccgta cgaaagggcc gcgccgtccg gacccccctc gtcctcgggg cgcgcggcgg
     4501 gaaggtcgcg gaggtgctgc agggcctcga cgagggggag ctcgtcgtcc gcgagcccgg
     4561 ccgcgtcgag gagggccgga gggtccggac gcgcctcgcg tcggggggct gacgtgccgt
     4621 tcgaggtcct cgtcgccctc cgcttcctcc gcgaggggcg gatccagacg ctcctgatcc
     4681 tctccggcat cggcgtcggc gtcggcgtga tcgtcttcct ctccgccctc atctcggggc
     4741 tccaggcgtc tctcatcgag cggacgctcg ggacgcaggc gcatgtcgtc gtccgggcgc
     4801 cggaggacgt cgttcggccg gcgaccgccc cgaccgcgga gcgcgtcgtc gcatcccgcc
     4861 tcgagcagcc cgcgcaacgc ctccggtcga tcgtgggctg gccccagacc ctcggcctcc
     4921 tgcgcagcct ccccgggatt cgcgcggcgt ccccgaccgc cgaggggccg gccgtcgccc
     4981 tccgcgggct cgcgaaccgc tcgatcgccc ttcgcggggt agagccggcg agctaccgcg
     5041 ggatcatcga catgcccccc cggatgaccg atggagagtt ccgggtcgac gcggcgcacg
     5101 ccgtcctcgg cgtggagctc gcgaaggacc tcgggctgag gaagggagac aagctccgga
     5161 tcgagacccc gcgcggccgc tcggacgtct tcgtcgtcgc cggcctcttc gacgtcggca
     5221 acaaggacct gaacgagcgc tgggtcttcg tctccctccg ctcggcccag acgctcctcg
     5281 acctctcggg ggggatctcg tcgatcgagc tgaaggtcga cgagctcttc gccgcggacc
     5341 ggatcgccgc cgacatcgcg ggccggaccg gcctcgaggc cgagagctgg atggagcgga
     5401 accggcagct cctcgtcggg ctccggtccc agagcgcctc cagcgggatg atccagctct
     5461 tcgtcgtgat cgccgtcgcc ctcggcattg cgagcgtcct cgtcgtctcc gtcgtgcaga
     5521 agtcacgcga gatcggcatc ctgaaggcga tggggacgcg gacggcgcag gtcgtccgtg
     5581 tcttcctcgt tcagggggcg gtactcgggc tggccggctc ggccttcggg atcctgctcg
     5641 gaagcgccct cgcgctcttc ttcgccgcgc tcgcgacgaa ccccgacggc tccccgacct
     5701 tcccggtcgc cctcaccccg ggcctcttcc tcggcgccgc cgccgtcgcg acggtcacgg
     5761 ggctcgtcgc cgccgtcgcc cccgccgagc gggccgcctc gctcgacccc gccgacgtga
     5821 tccgccatgg ctgagccgct cgtcctcgtc gaaggggtga cgaagtcctt cggccgcgag
     5881 gtcgtgacga aggtcctcca cggggtcgac ctcgagctcc acccggcgga gttcacggcg
     5941 ctcgtcggac cgtccggttc cggcaagagc acgctcctga acctcctcgg cctgctcgac
     6001 cgtccgacgt cgggccggat cgtcctcgag ggtcgcgaca cgacggacct cgccgaggcg
     6061 gagaggacca ccctccgggg ccggtccctc ggcttcgtct tccagttcca ccacctcctg
     6121 ccggctttca ccgcgctcga gaacgtgatg atgccgatgc tcgccgcgcg cgggcgcgag
     6181 gagcccggga tgcgcgagag ggcattcgcc ctcctggaag agatcggcct cgccgaccgg
     6241 gcctcctacg gcgcctcgaa gctctcgggc ggccagcagc agcgcgtcgc ggtcgcccga
     6301 gccctcgtcc tcgatcctcc gctcgtcctc gccgacgagc cgaccggcaa cctcgacacc
     6361 gagtcgggcg c
//
LOCUS       DAOTEZ010000241         6367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_8732_length_6367_cov_5.260139, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000241 DAOTEZ010000000
VERSION     DAOTEZ010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6367
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8732_length_6367_cov_5.260139"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4355)
                     /locus_tag="P5164_12760"
     CDS             complement(<1..4355)
                     /locus_tag="P5164_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44806.1"
                     /translation="MLEPLRPRRPFLVALAVSTTLAAATGRAALDAVDALVPWTELTA
                     ADALASTLVRKAVVDGRTVHYPTPTKELAVELEARAASEGRAAVAALRHLAEARRELG
                     DLPGGESALTRWAEGTGGAAWAEAARWGALYRRWPFAFASARAALASDAPAATKRALA
                     TERIAWADEDPSRGDPLALRAERAALFPDDAAFVEEWIRALEKAGRLDEADAALRGAK
                     GLPEETRLLVLSDLKGDHGDREGAYSVLEAWVAAPERVPSRRALEAFSRRADAVAASR
                     MESARLALESRFDPRGLTLLARWFEGKGRADLALELLAQVELRHEEGFTRPDRIVLAR
                     LYEALDAVPEAFRSRLAGATAASADEAPDDLAALARLAFAAGSRPIAWGALNDEPYRW
                     AARADVTPGFATAGLSFLLTGLDPQNALAELEAQRLPEKTLRAGRLLLAELERRKPEH
                     AAIPALHVTLMERLVSRGKGAEALALLPRAESGDAATRAEARRVALLAMRQAKTPLEK
                     ELPLWKERLALLAPDGSVPGAGAGDRSDAEGSEPSGEGPGWEGDDGERPEKERLATAP
                     KDDEYDRVLSEALSRLDDRDRTHRASVSLLLGEMDRLPRAETVWLKAVDRLSGWKLDD
                     ELEPRYRKAIATFEGPEWWKRLARWYARRQRAAELRALGEDLVASFRASELFARDPML
                     DDALVAVEDQPNPFVTFSDFLALRALQRFPASSAALDRAEARLLTRASFDDLLARRPG
                     EAKRRAVVDAALLASRRDAVLFADDSRRSRFLDTLVKNGELESLLRRLEEVPGKTPVE
                     DLLLRDGWARLSRFERAVPFAEALCEAYPGSAAHAAAAIALERSLSALSSDRAAAAGR
                     IAVRAAAAASDPSPFLTPIGEMWQDLERPEPAGHAFREVLAASPRNPDTILEVATAFW
                     DYGRFAEAWQVLDEGRKRLGRPGLHAFEAGVLKEELRDRDGALAEYVAALSGEWDAGA
                     RARRRLARLAGRPVVRDLLLARIDRLAPGVPADESALASYLPLATLSPAETSEWDDWM
                     DLPRDPVGRAERAEKRETARPSEETGAAVVGAAIWRKAREMAPRASSPQLLAALRTWS
                     SSVADARWAGSEGATTLEGLLLEREAALLPSEEARIPKEAARIDWLLARGRAEEATAA
                     WAKLLPRVEALPDGATKIRALAARARFLEFAGGDAAGAWRDAVRRFPWSLGLVEDQVA
                     FCLRTGRGAEGLDALESAASRAAAGHREPLTRRLVAAALERKDLPRARRAVERLLAYD
                     LDPSARVDALALSVRLAFREGAGVDPFALAKSEAPKLPEALRPDLWAALAAGARDEGR
                     LATGVDLYVEALNRRTDREWLAAACRLAVRAGKADDLLAFFRAQRKRSPRDVRWAVAV
                     REVLTFRGDLEGATAAAREAISIAPEREELYAETVALLERQGRFRDAADVLAGRAKSR
                     PADESIASWRAALHVR"
     gene            complement(4355..>6367)
                     /locus_tag="P5164_12765"
     CDS             complement(4355..>6367)
                     /locus_tag="P5164_12765"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44807.1"
                     /translation="KRARALYEELTTRRIDPGLLQQGEEEDTSSGGPGRPSGGAVFSV
                     KVSPIPGWGTKRLELQYQEEVPWADGVGELLIPLRPGSGEPTVAGSLEVSVTLEDGSF
                     LEVPATALPLAVAGRVARFRGSAVPLAKDVVVRFRPASSEPLTFSAFRSPDGRLPDGL
                     ALAPWERLSDVPPEKDGFFLLEHRPAAEKGTAPDAARDRPPLHVAVLFDTSLSSRWGS
                     LESGWAFLTRLLDRLGPQDRFVVVPFDRTPAPDPAGLAAATAAAKEAALSYLRTRPLG
                     PGTDVAAALRAAAALVPSGSDARLVLVTDGVDAPGALAASAAGRPLYTVLTGEERREA
                     FAVSSRQLLPLPPGSTLQPPPGEALFLAQLFAPLPKEEPREGGASGLPFVRKGEDPQL
                     RDVYGVMTQPPLAGSLSGWVGRYARPARATLELGGASVVASFAERSLEARDLPRRWAR
                     ARVDDLLRRIELEGEKREWVEEILELSRRYKLVTPYTAFLAAPRALLRPRRIQPGDPV
                     IRIDALPGTAAVAALLPFGRKLDLRRRPGTSLWEGRFLVPDGTKDGPMNVRIVVRDRN
                     GATAVETKRAVVDGTPPTVTPLLPASAAAGSRVRLAARADADVVALTARVGDGVPVPL
                     RWDGAAKANVAELFLPQGLTGAVPVHFEAIDGAGNHGFARAELRVR"
BASE COUNT          808 a         2262 c         2578 g          719 t
ORIGIN      
        1 cggacgtgga gagccgcgcg ccacgaggcg atcgactcgt ctgcggggcg gctcttcgcc
       61 cggccggcga ggacgtcggc cgcgtcgcgg aagcgcccct ggcgctcgag gagagcgacg
      121 gtctcggcgt agagctcctc cctctccggg gcgatcgaga tcgcctcgcg cgcagcggcc
      181 gtggcgccct cgaggtcgcc gcggaacgtc aggacctcgc ggacggcgac ggcccagcgg
      241 acgtcgcgcg ggctcctctt ccgctgcgca cggaagaagg cgaggagatc gtcggccttc
      301 ccggcgcgga cggcgaggcg gcaggccgcc gcgagccact cccggtcggt gcgccggttg
      361 agcgcctcga cgtagaggtc gaccccggtc gcgagccgcc cttcgtcgcg ggcgcccgcg
      421 gcgagcgcgg cccagaggtc cggccgcagg gcctccggaa gcttcggcgc ctcggacttc
      481 gcgagggcga acgggtcgac gcccgccccc tcgcggaagg cgaggcggac ggagagcgcg
      541 agggcgtcga ctcgggcgga cgggtcgagg tcgtacgcga ggaggcgctc gacggcgcgg
      601 cgggcgcggg gcaggtcctt cctctcgagc gcggcagcca cgagccgccg cgtgagcggc
      661 tcgcgatgcc cggccgcggc gcgtgaggcg gccgactcga gggcgtcgag cccttcggcg
      721 ccgcggcccg tccggaggca gaaggcgacc tggtcctcca cgaggccgag cgaccagggg
      781 aatcggcgga cggcgtcgcg ccacgcgccg gccgcgtcgc ctccggcgaa ttcgaggaag
      841 cgggcgcgcg ctgcaagggc gcggatcttc gtcgcgccgt cggggagagc ctcgacgcgc
      901 gggaggagct tcgcccaggc ggccgtggcc tcctcggcgc ggccgcgggc gagaagccag
      961 tcgatgcggg cggcttcctt cggaatccgc gcctcctcgc tcgggagcag cgcggcctcg
     1021 cgctcgagga ggagcccctc gagcgtcgtc gctccctcgg agccggccca ccgggcgtcg
     1081 gcgacgctcg acgaccaggt ccgcagggcg gcgagaagct ggggcgagga ggcgcggggc
     1141 gccatctccc gagccttccg ccagatcgcg gcgccgacga cggcggcgcc cgtctcctcg
     1201 gaggggcgcg ccgtttcgcg cttttccgcg cgctccgccc ggccgaccgg gtcgcgcggc
     1261 aggtccatcc agtcgtccca ctcggacgtc tctgccgggg agagagtcgc gagggggagg
     1321 tacgacgcga gggcggactc gtccgcgggg actccggggg cgagccggtc gatccgggcg
     1381 aggaggaggt cccggacgac gggccgaccg gcgagacggg cgaggcgcct tcgggcgcga
     1441 gcgccggcgt cccactcgcc ggagagcgcc gcgacgtact ccgcgagcgc gccgtcgcgg
     1501 tcgcggagct cctccttgag gacgcccgcc tcgaacgcgt ggagcccggg gcggccgagc
     1561 cgcttccgcc cctcgtcgag gacctgccac gcctcggcga agcggccgta gtcccagaac
     1621 gcggtcgcga cctcgaggat ggtgtcgggg ttccgagggg aggcggcgag cacctcgcgg
     1681 aacgcgtgac cggccggctc gggccgctcg aggtcctgcc acatctcgcc gatcggcgtc
     1741 aggaatggcg acgggtcgga cgcggcggcc gcggctcgga ccgcgatccg cccggcggcg
     1801 gccgcgcggt ctgacgaaag agccgacagg gaccgctcga gggcgatcgc cgcggcggcg
     1861 tgtgccgcgc ttcccgggta ggcctcgcag agagcttcgg caaacgggac cgcgcgctcg
     1921 aatcgcgaga ggcgcgccca gccgtcgcgc aggaggaggt cctcgacggg agtcttcccg
     1981 gggacctcct cgaggcggcg aaggagcgac tcgagctcgc cgttcttcac gagcgtgtcg
     2041 aggaagcggc tccggcggga atcgtcggcg aacaggacgg cgtcgcggcg cgacgcgagg
     2101 agcgcggcgt cgacgacggc ccggcgcttc gcctcgccgg gcctccgcgc caggaggtcg
     2161 tcgaaggagg cgcgcgtcag gagccgcgcc tcggcgcggt cgagcgcggc ggaactcgcc
     2221 gggaagcgct gcagagcgcg cagggcgagg aagtcggaga aggtgacgaa ggggttcggc
     2281 tgatcctcga ccgccacgag ggcgtcgtcc agcatcgggt ctcgggcgaa gagctcggag
     2341 gcgcggaagc tcgcgacgag gtcctctccg agcgccctca gctcggcggc tctctgccgc
     2401 cgagcgtacc agcgggcgag acgcttccac cattcgggcc cctcgaaggt cgcgatcgcc
     2461 ttccggtagc gcggctcgag ctcgtcgtcg agcttccagc cggagaggcg gtcgacggcc
     2521 ttcagccaga ccgtctcggc ccgcgggagg cggtccatct cgccgaggag gagcgagacg
     2581 ctcgcgcggt gcgtgcggtc gcggtcgtcg agccgggaga gcgcttccga gaggacgcgg
     2641 tcgtactcgt cgtccttcgg agccgtggcg aggcgctcct tctccgggcg ctcgccgtcg
     2701 tccccctccc accccggccc ttcgccggac ggctcggagc cctcggcgtc ggagcggtcg
     2761 ccggccccgg cgcccgggac gctcccgtcg ggcgcgagaa gcgcgaggcg ctccttccag
     2821 agcgggagct ccttctcgag aggggtcttc gcctgtcgca tggcgagcag agcgacccgc
     2881 cgcgcctcgg cgcgggtggc cgcgtcgccc gactcggcgc gcggaaggag ggcgagcgcc
     2941 tcggccccct tgccgcggga gacgaggcgt tccatgagcg tcacgtggag ggccgggatc
     3001 gccgcgtgct ccggctttcg cctctcgagc tcagcgagga ggagccggcc ggcacggaga
     3061 gtcttctccg ggaggcgctg cgcctccagc tcggccaggg cgttctgcgg gtccaggccg
     3121 gtgaggagga acgagagccc ggccgtggcg aagccggggg tgacgtcggc gcgggccgcc
     3181 caccggtacg gctcgtcgtt gagcgcgccc caggcgatcg ggcgggagcc ggcggcgaag
     3241 gcgaggcgtg cgagggccgc gaggtcgtcg ggcgcctcgt cggcggaggc ggccgtcgcg
     3301 ccggcgagcc ggctgcggaa cgcctcgggg acggcgtcga gcgcctcgta gaggcgcgcg
     3361 aggacgatcc ggtcggggcg cgtgaagccc tcctcgtgac gcagctcgac ctgggcgagg
     3421 agctcgagcg cgaggtcggc gcgccccttc ccctcgaacc accgggcgag gagcgtgagg
     3481 ccgcgcgggt cgaaacggct ctcgagcgcg aggcgggccg actccatccg cgaggcggcg
     3541 acggcgtcgg cgcgccgcga gaacgcctcg agggctcgtc gcgacgggac gcgctccggg
     3601 gcggcgaccc aggcctcgag gacggaatac gccccctccc ggtcgccgtg atctcccttc
     3661 aggtcggaga ggacgaggag gcgcgtctcc tccgggagtc cctttgcgcc tcggagcgcg
     3721 gcgtcggcct cgtcgagacg ccccgccttc tcgagggcgc ggatccactc ctcgacgaag
     3781 gcggcgtcgt cggggaagag ggcggcgcgc tcggcgcgca gcgcgagggg atccccgcga
     3841 gacgggtcct cgtcggccca ggcgatccgc tccgtcgcga gcgcgcgctt cgtcgccgcc
     3901 ggtgcgtcgg aagcgagcgc ggcgcgcgcg gaggcgaacg cgaacggcca gcgccggtag
     3961 agcgctcccc agcgcgccgc ctcggcccag gcggcgccgc cggtcccttc ggcccagcgc
     4021 gtgagcgcgg attcccctcc cgggaggtcg ccgagctcgc ggcgcgcctc ggcgagatgg
     4081 cgaagggccg cgaccgccgc tcggccttcg gaagcggcgc gcgcctcgag ctcgacggcg
     4141 agctccttcg tcggggtcgg gtagtggacc gtgcggccgt cgacgacggc cttgcggacg
     4201 agcgtcgagg cgagcgcgtc ggccgcggtc agctcggtcc aggggacgag ggcgtcgacc
     4261 gcgtcgagcg cggcccgccc ggtcgcggcc gcgagcgtcg tcgaaacggc gagagcgacg
     4321 aggaaagggc gtcggggacg gagcggctcg agcatcagcg gaccctcagc tccgcgcggg
     4381 cgaacccgtg gttccccgcg ccgtcgatgg cctcgaagtg gaccgggacc gcgccggtga
     4441 ggccctgcgg caggaagagc tcggcgacgt tcgccttcgc ggcgccatcc cagcggagcg
     4501 gcaccgggac gccgtcgccg acgcgcgcgg tgagcgcgac gacgtcggcg tcggcccggg
     4561 ccgcgaggcg cacgcgcgag cccgcggcgg ccgaggccgg caggaggggc gtcacggtcg
     4621 gcggggtgcc gtcgacgacg gcccgcttcg tctcgacggc cgtcgcgccg ttccggtcgc
     4681 ggacgacgat ccggacgttc atcggcccgt ccttcgtccc gtccggtacg aggaagcgcc
     4741 cttcccagag gctcgtgccc gggcggcgcc ggaggtccag cttccggccg aacggcagga
     4801 gcgcggcgac ggcggccgtt cccggcaggg cgtcgatgcg gatcaccggg tcgcccggct
     4861 ggatgcgccg cggacgaagg agagcgcgcg gcgcggcgag gaaggcggtg tagggcgtga
     4921 cgagcttgta gcggcgcgag agctcgagga tctcctcgac ccattcacgc ttctccccct
     4981 cgagctcgat ccggcgcagg agatcgtcga cacgcgcgcg ggcccagcgg cgcggcaggt
     5041 cgcgcgcctc gagagacctc tcggcgaagc tcgcgacgac ggaggcgccg ccgagctcga
     5101 gcgtcgccct cgccgggcgc gcgtagcggc cgacccaccc ggagagcgaa ccggcgagcg
     5161 gcggctgcgt catcactccg tagacgtcgc ggagctgcgg gtcttcgccc ttccgcacga
     5221 aggggagccc cgacgcccct ccctcccgcg gctcctcttt cgggagcggt gcgaagagct
     5281 gcgcgaggaa gagcgcctcc ccgggcggcg gctggagcgt gctgccgggc gggaggggga
     5341 ggagctggcg cgaggagacg gcgaaggctt ccctacgctc ctcgccggtc aggacggtgt
     5401 agagaggacg gcccgccgcg gatgcggcga gcgccccggg cgcatcgacg ccgtccgtca
     5461 cgaggacgag gcgcgcgtcg ctcccggacg gcacgagcgc ggcggcggcg cgaagggcgg
     5521 ccgcgacgtc ggtcccgggg ccgagagggc gcgtgcggag gtaggagagc gcggcctcct
     5581 tcgccgcggc tgtggcggcg gcgaggccag ccgggtccgg agcgggtgtc cggtcgaacg
     5641 gcacgacgac gaaccggtcc tggggcccga gccggtcgag gagccgggtc agaaacgccc
     5701 agcccgactc gaggctcccc cagcggctcg agagggaggt gtcgaagagg acggcgacgt
     5761 ggaggggggg gcggtcccgc gcggcgtccg gagcggtgcc cttctcggcc gccggccggt
     5821 gctcgagaag gaagaagccg tccttctcgg gcgggacgtc ggagagccgc tcccaagggg
     5881 cgagggcgag gccgtccgga aggcggccgt ccgggctccg gaacgccgaa aacgtgagcg
     5941 gttcggagga ggcggggcgg aagcggacga cgacgtcctt cgcgagcggg accgcgcttc
     6001 cgcggaaccg ggcgacccgg cccgcgacgg cgagcggaag cgccgtcgcg ggcacctcga
     6061 ggaaggagcc gtcctcgagc gtgacgctca cctcgaggct ccccgcgacc gtcggctcgc
     6121 cggagcccgg gcggagcgga atgaggagct cgccgacgcc gtcggcccag gggacctcct
     6181 cctggtactg cagctcgagc cgcttcgttc cccagccggg gatcggggag accttcacgg
     6241 agaagacggc tcctcccgac ggccgtcccg gcccgccgga gctcgtgtcc tcctcctctc
     6301 cctgctggag caaccccggg tcgatccggc gcgtcgtgag ctcctcgtag agggcgcgtg
     6361 cccgctt
//
LOCUS       DAOTEZ010000242         6366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_8734_length_6366_cov_5.576454, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000242 DAOTEZ010000000
VERSION     DAOTEZ010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6366
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8734_length_6366_cov_5.576454"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1502)
                     /locus_tag="P5164_12770"
     CDS             complement(<1..1502)
                     /locus_tag="P5164_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44808.1"
                     /translation="MTTEPRVRSVFVLSAVVVLLAGGALALGVKPGAAKGVKPVPKAA
                     STWKDVDRLVDEQKLEEAAKAVEAILSAAKAAKDEPEWTKGLVKVTQLRIGLHGYETA
                     VRRLREEPWPAGPLHRAQLSLLYGHSLVTYQRAYSWEIGQRERVDTKGEVDLKAWTRE
                     QILAEALKAALEVWAIRSDLGKEPVSAAALVLVPNDYPKEIRGTLRDAASYLLVDLLA
                     DTAGWTPEQSNEVWRLDLGALLAGDAEREGKTDLASPEVHPLVRIGAVLTDLEAWHTK
                     EGRTGARLEARLERVRRLAASFTEERDRERIRKDLEGRLVAFRSDPWWAMGKAEVAEL
                     HRNGARQSRMVEARRVAEEGRAAYPSSPGGARCLAIVKAIEAPEYALQAMAVDGAKKR
                     SVEVSHRNLEALHFRAFRVDAEKWIAASKDWSALPSSEDMRKLSGGAPAAEWTSALPK
                     TADFETHRTFVTPPLPEPGFYVLLASARKDFRESDNVVVGTNLFVSDLVLA"
     gene            complement(1581..2333)
                     /locus_tag="P5164_12775"
     CDS             complement(1581..2333)
                     /locus_tag="P5164_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015735432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRY44809.1"
                     /translation="MVVRPRRVSGLLSLVGASLAASCGTLEPTPAAGLSARRFEGTVS
                     YRVAYPYLLYLPEGYRRDPASRWPLLLFLHGSGERGDDLALVKRHGPPKLLASGGALP
                     FVVVAPQCPEGERWRADALAALLDDVTRRLRIDAERVYVTGLSMGGRGTWDLAMSHPG
                     RFAAIAPVCGGALPDRACNLKGVPVRAFHGAKDDVVPLSESERVVEAVRRCGGTAELV
                     VFPEAGHDSWTAAYSDPSLWDWLLSHRRRAAR"
     gene            complement(2377..4794)
                     /locus_tag="P5164_12780"
     CDS             complement(2377..4794)
                     /locus_tag="P5164_12780"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HRY44810.1"
                     /translation="MARDVAPSGAPRIGTKVLHKFNRDLGPGRVEEVAARRVVVFFPR
                     SGERLTFGIADTGLRPLELSAGRDARIEETGEVVTLASRERGGTSWTTTDGRVLEEER
                     LWPAEIPQDPVERLAGLDVDRAAAFRNRVDGLFLSRMRQAKGLGSFLGGRIAIHPHQL
                     HVAERATAYEPVRWLLADEVGLGKTIEACLILSRLVRTDRADRVLVVAPSTLTVQWLG
                     ELWRKFHQVFVLLDAKRRADVAKDHGPEFNPFDAHRRSVIALEDLASEPGLAQKAVEA
                     GLDLLVVDEAHRLVLEAEAERAVAPLAKAARHLLLLSATPLEADTSGFFRLLRMVRPD
                     AYRSEKEFVAALSENQPLPPCTSATRRVDIGGLPPRVPLPVELDRVPTSQALSGEEKA
                     DAVDPRVEWLVGKAKGFAPGGAEEGKTLVFCHDLPTLQSLKALLERETRKRVAVFHEE
                     LSPDRRDLEVAEFRRPKGPTFLISTECGGEGRNFEFCRRLVLFDLPLDPAQVEQRIGR
                     LDRISRKERVEIVYFRPPSGFWRDLVELYERIGLFREPLGGLERSLANVEAAIRKAEK
                     ASALGRAELPVGELVAEVRGAAQARQRAAYHHLHAEGYRPEHASGIVSRVPAGLDAAM
                     ERFVIAACELYGFDVAEKKGSRTWYLEFGGDALLEHLPGVPGGSRFLGTFDREEAVRH
                     EERDFFASGHPLVEGIFQELEDGPRGRAALVKLEKSGAAGEGVLFLHRREGGFAASAV
                     DLSGQSRPEWAELLLRGRASLRGISVDDWSPTIPRGRGWAATVRTLAARAEKNGPVLA
                     AAAFRLS"
     gene            complement(4865..5689)
                     /locus_tag="P5164_12785"
     CDS             complement(4865..5689)
                     /locus_tag="P5164_12785"
                     /inference="COORDINATES: protein motif:HMM:NF020772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HRY44811.1"
                     /translation="MVPRPRYDADWDYLPDYWKTAGVRRNTGIKYYRITGRGADLGGK
                     TYYVPEWADETREAQAAQFVFERGAQATWFQEKHGGRKPCVVSAYDAELFGHWWEEGP
                     GFLESVFRKLLWDQSVVRPVTPSEYLSENPHHQRMTPGASSWGKGDYFQTWVEGREYQ
                     PNTWVYRHLFRLVGKMADLATRHRDTVDLLLKRALDQAARCLFLAGASDWGFLIETGQ
                     AVRYSEVQIVRHLDRARELMRQVDAGKIHEDYLTILEDADTVFPFGDMDFRVFCRP"
     gene            6031..>6366
                     /locus_tag="P5164_12790"
     CDS             6031..>6366
                     /locus_tag="P5164_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44812.1"
                     /translation="MRRRAGDDLELPGVLEVAIPARQVSLVGVPDVLRRPEAREDLLR
                     RREPRLAARVFPLPRRKLEALVDVPGRAELQVRVLHHREERRREADGEAEVDLVALQV
                     PEDVEELRVD"
BASE COUNT          906 a         2287 c         2262 g          911 t
ORIGIN      
        1 gcgaggacga ggtcggagac gaagaggttc gtcccgacga cgacgttgtc cgactcgcgg
       61 aagtccttcc gcgccgaggc gaggaggacg tagaaccccg gctcgggcag gggcggcgtg
      121 acgaacgttc ggtgcgtctc gaagtcggct gtcttcggga gcgcggacgt ccactcggcc
      181 gcgggcgccc cgccggagag cttccgcatg tcttccgagg aggggagcgc cgaccagtct
      241 ttcgacgccg cgatccactt ctcggcgtcg acgcgaaagg cccggaagtg gagcgcctcg
      301 aggttccggt gggagacctc gacggaacgc ttcttcgccc cgtcgacggc catcgcctgc
      361 agcgcgtact ccggcgcctc gatcgccttc acgatcgcga ggcacctcgc cccgccggga
      421 gagctcgggt aggccgcgcg tccctcctcc gccacccgcc gcgcttcaac catccggctc
      481 tgacgcgcgc cgttccggtg gagctcggcg acctcggcct tccccatcgc ccaccacgga
      541 tcggagcgga acgccacgag gcgcccctcg aggtccttcc ggatccgctc ccggtcgcgc
      601 tcttcggtga acgacgccgc cagccttcgc accctctcga gacgcgcctc gagccgcgct
      661 ccggtccgcc cctccttcgt gtgccacgcc tccaggtccg tgaggaccgc gccgatcctc
      721 acgagcgggt ggacctccgg agaggcgagg tccgtcttcc cctcgcgctc cgcgtctccc
      781 gcgaggagcg ccccgaggtc gagcctccag acctcgttcg actgctccgg cgtccagccg
      841 gcggtgtcgg cgaggagatc gacgaggagg tagctcgccg cgtcgcggag cgtcccgcgg
      901 atctccttcg ggtagtcgtt cggcacgagg acgagcgcgg ccgcggagac cggctccttc
      961 ccgaggtcgg agcgaatcgc ccagacctcc agcgccgcct tcagcgcctc ggccaggatc
     1021 tgctcccggg tccaggcctt caggtcgacc tcccccttcg tgtcgaccct ctcgcgctgg
     1081 ccgatctccc aggagtaggc ccgctggtag gtgacgagcg agtggccgta gaggagcgag
     1141 agctgcgccc ggtggagcgg cccggccggc cacggctcct cgcgcaggcg cctcacggcc
     1201 gtctcgtagc cgtggaggcc gatccggagc tgcgtgacct tcacgagccc tttcgtccac
     1261 tccggctcgt ccttcgccgc cttcgcggcc gaaaggatcg cctccacggc cttcgccgct
     1321 tcctcgagct tctgctcatc cacgagacga tcgacgtcct tccaggtgga ggcggccttc
     1381 ggcacgggct tcactccctt cgcagctccc ggcttgacgc cgagcgcgag cgcgcctccg
     1441 gcaaggagaa cgacgacggc ggacaggacg aatacggacc ggacgcgcgg ctctgtcgtc
     1501 ataacgagag cttagaccgc ctcgcccccc ggaagttcct ccgacacggt ccgactcggt
     1561 gccaggcgtg aaatcgtgta ttaccttgcg gctcgacgcc ggtgggagag gagccagtcc
     1621 cagagggacg ggtcggagta ggccgccgtc cacgagtcgt gccccgcctc cgggaagacg
     1681 acgagctccg ccgtgccacc gcaccgacgg accgcctcca cgaccctctc cgattcggac
     1741 agcgggacga cgtcgtcctt cgcgccgtgg aaggcccgga ccggcacgcc cttcaggttg
     1801 caggcgcggt cgggtagcgc tccgccgcag acgggtgcga tcgcggcgaa gcggccgggg
     1861 tgggacatcg caaggtccca cgtcccgcgt ccccccatcg agagccccgt gacgtaaacg
     1921 cgctccgcgt cgatgcgcag ccgccgcgtg acgtcgtcga ggagagcggc gagggcgtcg
     1981 gcccgccagc gctccccctc cgggcactgc ggcgcgacga cgacgaacgg aagcgccccg
     2041 cccgaagcga ggagcttcgg cggaccgtgg cgcttcacga gggcgaggtc gtctcccctc
     2101 tcgcccgacc cgtgcaagaa gaggagcagc ggccaccggc tcgcggggtc gcgccggtac
     2161 ccctccggga ggtagagcag gtacgggtac gcgacccggt aggagaccgt tccctcgaag
     2221 cgccgggcgc tcagccccgc cgcgggcgtc ggctcgagcg tcccgcacga ggcggccagc
     2281 gaggctccga cgagcgagag gaggccggag actcgtcgcg gccgaaccac catgcgccca
     2341 ttctgccgcg cctcgcggcc ggctgcttcc ggagcgtcag gagaggcgga acgcggccgc
     2401 ggccaggacg gggccgttct tctcggcccg cgccgcgagc gtccgaacgg tcgcggccca
     2461 gccccgtccc cgcggaatcg tcgggctcca gtcatcgacg gagattccgc gcaggctcgc
     2521 gcggccgcgc agaaggagct ccgcccactc ggggcgtgac tgacccgaga ggtcgacggc
     2581 ggaggcggcg aagcccccct ctcttcggtg gaggaagagg actccttctc ccgcggcccc
     2641 gctcttctcg agcttcacga gggccgcccg cccgcgcggg ccgtcctcga gctcctggaa
     2701 gatcccctcg acgagcggat ggccggaggc gaagaagtcg cgctcctcgt gccggaccgc
     2761 ctcctcgcgg tcgaacgtgc cgaggaagcg gctcccgccc gggacgcccg ggaggtgctc
     2821 gaggagggcg tcgccgccga attccaggta ccaggtccgg gagcccttct tctccgcgac
     2881 gtcgaagccg tagagctcgc aggccgcgat gacgaagcgc tccatcgccg cgtcgaggcc
     2941 cgccgggacg cgcgagacga tcccgctcgc gtgctcggga cggtacccct cggcgtgaag
     3001 gtggtggtag gcggcgcgct gacgcgcctg cgccgcgccc cggacctccg cgacgagctc
     3061 cccgacggga agctccgcac ggccgagggc cgacgccttc tccgccttcc ggatcgcggc
     3121 ctccacgttg gcgagcgacc gctccagccc gccgagcggc tcgcggaaga gcccgatccg
     3181 ctcgtagagc tcgacgaggt ctcgccagaa tcccgaagga ggacggaagt agacgatctc
     3241 gaccctctcc ttccggctga tccggtcgag gcgcccgatg cgctgctcga cctgtgccgg
     3301 gtcgagggga aggtcgaaga gcacgaggcg ccggcagaac tcgaagttcc gcccttcccc
     3361 gccgcactcc gtcgagatca ggaacgtcgg tcccttgggc cgtcggaact ccgccacctc
     3421 gaggtcgcga cggtccggtg agagctcctc gtggaagacg gcgacgcgct tgcgcgtctc
     3481 gcgctcgagg agcgccttca gcgactggag cgtcgggagg tcgtggcaga agacgagcgt
     3541 cttcccctcc tcggcccctc ccggagcgaa gcccttcgcc ttcccgacga gccactccac
     3601 gcgcgggtcg acggcgtcgg ccttctcctc tcctgaaagg gcctgggagg tcgggacccg
     3661 gtccagctcc accgggagcg ggacccgggg cggcaggcct ccgatgtcga cgcgccgcgt
     3721 ggcgctcgtg cagggaggaa gcggctggtt ctccgagagg gccgcgacga actccttctc
     3781 ggaccggtac gcgtcgggcc ggaccatccg gaggaggcgg aagaagcccg aggtgtccgc
     3841 ctcgaggggc gtcgcggaga ggagcaggag gtgtcgcgcg gccttcgcga gcggcgcgac
     3901 ggcccgctcg gcttcggcct cgaggacgag ccggtgcgcc tcgtcgacga cgaggaggtc
     3961 gagcccggcc tcgacggcct tctgggccag ccccggctcc gaagcgaggt cctcgagcgc
     4021 gatgacggag cgccggtgcg cgtcgaacgg gttgaactcc ggtccgtggt ctttcgccac
     4081 gtccgcgcga cgcttcgcgt cgaggaggac gaagacctga tggaacttcc gccagagctc
     4141 gccgagccac tggaccgtga gggtcgacgg cgcgacgaca aggacgcggt cggcccggtc
     4201 ggtccggacg aggcgcgaga ggatcaggca ggcctcgatc gtcttcccga ggccgacctc
     4261 gtcggcgagg agccagcgga ccggctcgta ggccgtcgca cgctcggcga cgtggagctg
     4321 gtgcgggtgg atcgcgatgc gtccgccgag gaacgagccg aggcccttcg cctggcgcat
     4381 gcgcgagagg aagagcccgt cgacgcggtt gcggaacgcg gccgcccggt cgacgtcgag
     4441 gccggcgagc cgttcgaccg ggtcctgcgg gatctccgcc ggccagagac gctcctcctc
     4501 gaggacacgg ccgtcggtcg tcgtccagga cgtcccgccc cgctcgcgcg acgcgagggt
     4561 cacgacctcg cccgtctcct cgatccgggc gtcccgtccg gccgacagct cgagcggacg
     4621 gagccccgtg tcggcgatcc cgaaggtcag ccgctcaccg ctccgcggaa agaagacgac
     4681 gaccctgcgg gccgccacct cttcgacccg tcccgggccg aggtcgcggt tgaacttgtg
     4741 caggaccttt gtcccgatgc ggggcgcccc ggacggggcg acgtccctcg ccatgaggcc
     4801 gggaaggatg caccggccgc ttccccgggg caaggccccg gggaagaccg gccggtgcaa
     4861 cgccttaggg acggcagaag acccggaagt ccatgtcgcc gaacgggaag accgtgtcgg
     4921 cgtcctccag gatcgtcagg tagtcttcgt ggatcttccc ggcgtcgacc tgcctcatca
     4981 gctcgcgggc gcggtcgagg tggcggacga tctggacctc ggagtagcgg acggcctggc
     5041 cggtctcgat gaggaacccc cagtcggagg ccccggcgag gaagagacac cgcgccgcct
     5101 ggtcgagcgc gcgcttcagg aggaggtcga ccgtgtcgcg gtggcgcgtc gcgaggtcgg
     5161 ccatcttgcc gacgagccgg aagaggtgac ggtagaccca cgtgttgggc tggtactcgc
     5221 gcccctcgac ccaggtctgg aagtagtccc ccttgcccca gctcgaggcg ccgggcgtca
     5281 tccgctggtg gtgcgggttc tccgagaggt actcggacgg cgtgacggga cggacgaccg
     5341 actggtccca gaggagcttg cggaagaccg actcgaggaa gcccggcccc tcttcccacc
     5401 agtggccgaa gagctcggcg tcgtaggcgg agacgacgca cggcttgcgg ccgccgtgct
     5461 tctcctggaa ccaggtcgcc tgcgcgccgc gctcgaagac gaactgggcc gcctgcgcct
     5521 cgcgcgtctc gtcggcccac tccgggacgt agtacgtctt cccgccgaga tcggcgcccc
     5581 ggcccgtgat ccggtagtac ttgatcccgg tgttccgccg gacgccggcc gtcttccagt
     5641 agtcggggag gtagtcccag tccgcgtcgt accgaggtcg cggtaccact ccttgtagtt
     5701 cgggtgcccc gggaatccgt tctcgcgcga ccagatcttc gcgcgcgtct cggggtcgat
     5761 cgggaacgcc gccgtcccgg cgggcgtgat caccgggttc gccgagtcga agaaggggcg
     5821 cgtgtctccc tcgcggaggc ccctcgggtt gacgaggaac cagcggatcc cttcctcggc
     5881 caggaaggcg tcgagccccg gagtccaggc gttctcggcg agccagatgc cgcgcggggt
     5941 tcggccgaag acccgctcgt actgccgctt cgcggtctgc acctgccccc ggatcgcctc
     6001 cgggaagtcc ttgtagagcg ggagtatcca gtgcgtcgcc gggcaggtga tgacctcgag
     6061 cttcccggcg ttctggaagt cgcgataccc gcgcgtcagg tctccctcgt aggcgtccca
     6121 gacgtgcttc gccgtccaga agcgcgagag gatctgctcc gccgccgcga gccacggctt
     6181 gccgcgcgcg ttttcccgct cccgcgtcgc aagcttgagg cactcgtcga tgtgccgggc
     6241 cgcgcggagc ttcaggtgcg ggtcctgcat catcgagagg agcggcggcg tgaggctgac
     6301 ggtgaggcgg aagtcgacct tgtcgcgctc caggttccag aggacgtgga ggagctgcgt
     6361 gtagac
//
LOCUS       DAOTEZ010000243         6330 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_8806_length_6330_cov_6.839203, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000243 DAOTEZ010000000
VERSION     DAOTEZ010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6330
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8806_length_6330_cov_6.839203"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="P5164_12795"
     CDS             complement(<1..614)
                     /locus_tag="P5164_12795"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRY44813.1"
                     /translation="MIDETLTMIPGPTPVHDRILDALARPTTSHVAPTFVAAFREALA
                     GFRTLCRSERGQPFVVAGGGTLAMEMALVNVVAPGEKLLVVSHGYFGDRFGDLAASFG
                     IEADVMKAEWGKAISPAEVAARLARGGYAAVTVSHVDTSTGTLAPVEGYVEALRGRPE
                     LFLLDGVCATGGVDEKFDAWGVDVLLTGPQKAIGAPPGLALCLFS"
     gene            700..861
                     /locus_tag="P5164_12800"
     CDS             700..861
                     /locus_tag="P5164_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HRY44814.1"
                     /translation="MGLGRALLCIFLPPLAVLDKGCGTLLLVSLLTLAGWVPGVIGAL
                     VVCSREKAW"
     gene            899..2299
                     /locus_tag="P5164_12805"
     CDS             899..2299
                     /locus_tag="P5164_12805"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HRY44815.1"
                     /translation="MSVSVFDLFKIGIGPSSSHTLGPMKAARTFAAGLDAGGLLAAVV
                     SVRVTLYGSLAATARGHATDRAVLLGLLGERPEEIDPGNVDALVAAVRRSGRLALLGR
                     HEVPFAEGEALRLELAPLPFHPNGLVLEAFGPAGEPLAARTFFSIGGGFVVEEGAADA
                     GATAETAVPHPYRTAEELLAACERTALGISGVVRLNEGAFRPEAETEAGLLRIWDVMQ
                     ECVRRGCRAEGALPGGLGVKRRAPALFRKLTEFPESGLADPLTAIDWVSLYALAVNEE
                     NAAGSRVVTAPTNGAAGILPAVLHYYRRFVPGATDAGVVRMLLTAGAIGLLCKEGASI
                     SGAEVGCQGEVGSACSMAAGALAEALGGTPEQAANAAEIAMEHNLGLTCDPVGGLVQV
                     PCIERNAMAAVKAINAARLALRGDGQAFVSLDDVIRTMRDTGLDMKSKYKETARGGLA
                     ARLAAHANVSVGLPDC"
     gene            2340..3023
                     /locus_tag="P5164_12810"
     CDS             2340..3023
                     /locus_tag="P5164_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44816.1"
                     /translation="MFVGHFGVGFAGKAAAPRVSLGTLVLASQFIDLLWPTLLLLGLE
                     RVEVRPGITRVTPLDFVSYPISHSLLAVVLWGALFAGVYHLARRSAAGSAVLFGAVVS
                     HWLLDLLTHRPDLPLLPGSGPKIGLGLWGSLPGTLVVELALFAAGVALYVRATRPRDR
                     AGTWALAGLVAFLLVVYAANLFGGAPPDTRAIAWAGHAQWLLVLWAYAIDRHRIPAEA
                     AGPRAIPAA"
     gene            3034..3969
                     /locus_tag="P5164_12815"
     CDS             3034..3969
                     /locus_tag="P5164_12815"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY44817.1"
                     /translation="MNAASLLGPLACLGSAAFWALAANVYARVARDHGPMAVNFMRAA
                     LSFPLFLAVALLARPATGLALVTPGRAGWLLLSVVGSYVVGDALFLASARSLGVPVSL
                     AIASTYPLWSAFAGWAFLGQSVGAAGLAGVGLVVAGVVTVIGRGRGRDEGPDASDGAS
                     AGRSARPGTAGVLLALATSLFWALNTFAVTKGGADLPVAAVNVFRMGFAAALLPAGTL
                     LMRRRPSSLVVPAAEMKRVWPVFLLESVGGTALFVVGLTHTPLAVAAALTSLAPALSV
                     PFALVIGRERLDARTLAGIFVVTAGIVLLVAFGRG"
     gene            complement(4031..4768)
                     /locus_tag="P5164_12820"
     CDS             complement(4031..4768)
                     /locus_tag="P5164_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44818.1"
                     /translation="MKRIPVLSALLLLASASAAPAADLTTLQKAYFASTKPGSWARYE
                     MTSTDPKGKVQKSEMTMSRLGGDGDRVWFEIRSVPKEGSKGKPATIKYLLDTGFKVEK
                     NALDYMKYVESMIFQVDGEEAMEYPPDMMRQVAAAFVSNVDYGADVTSLGPCMTDGKT
                     GEKVVIRGSFDVKVVIVRMKGTTETELCLSEAVPFGRLSEKTVTKDDKGRLTSTTESR
                     LVDSGTGATSAIKGPVKKVELPKMPWG"
     gene            complement(4912..5904)
                     /locus_tag="P5164_12825"
     CDS             complement(4912..5904)
                     /locus_tag="P5164_12825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY44819.1"
                     /translation="MEPGAAPECRLCSGEMRPFLDVDERFSLARCAACGLVSTRPELD
                     EEAVGAYYPPEYYGKRNRRFHPLLERLIVVFRGRRARLVDRRARPGSVLDVGCGRGLL
                     PAQLRALGREACGTELSEGAATHAREVLGIPVFVGPLEESPYEDASFAAVVFWHVLEH
                     LRDPRAALRCAWRLLAPGGLLVVAVPNLESLQARVGGRGWFHLDVPRHYHHFGLAVLA
                     RLLDEEGFDVEEVSHFALEQNPYGWIQSLLNRLGLPANLLYAALKRDGAREVEHPVRS
                     APFAFALTLLALVAVLPFSGLLFTAELLLRRGGTVEVWARRREAPAPDAARRRP"
     gene            complement(5910..>6330)
                     /locus_tag="P5164_12830"
     CDS             complement(5910..>6330)
                     /locus_tag="P5164_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44820.1"
                     /translation="KKAVYMGYAFLYGVFGSLFGSSLGGALYGAWLKPLAGTPAGLDA
                     SRTFWLLFVALDVVAVVGLLLYNRFFSSDTPEANARARSIMTGLYGLFVVVGGFFLWK
                     ALFGGPEISYKTLVQALILTLLGVGGVLISRRKPAGA"
BASE COUNT          780 a         2282 c         2365 g          903 t
ORIGIN      
        1 gagaagaggc agagcgcgag gccgggaggg gcaccgatcg ccttctgcgg cccggtcagg
       61 aggacgtcga cgccccacgc gtcgaacttc tcgtcgacgc cgcccgtggc gcagacgccg
      121 tcgaggagga agagctcggg gcgccccctc agcgcctcga cgtatccctc gacgggggca
      181 agcgtcccgg tagacgtgtc gacgtggctg acggtgacgg ccgcgtagcc gcctcgggcg
      241 aggcgggccg cgacctcggc gggcgaaatc gccttgcccc actcggcctt catcacgtcg
      301 gcctcgatgc cgaaggaggc ggcgaggtcg ccgaagcggt cgccgaagta gccgtgggag
      361 acgacgagga gcttctcgcc cggcgcgacg acgttcacga gcgccatctc catcgcgagg
      421 gtcccgcccc cggcgacgac gaacggctgc ccgcgctcgc tccggcagag cgtgcggaag
      481 ccggcgagcg cctcgcggaa ggccgcgacg aacgtcgggg cgacgtgcga cgtcgtgggg
      541 cgggcgaggg cgtcgaggat ccggtcgtgg accggtgtcg ggccggggat catcgtcagc
      601 gtctcgtcga tcacgggtgg ctcctttccg gggcgggagg ggcgtgccga cgtccggccg
      661 gcgtcgcggc cgggccgccg acgaggagag aatagacgga tgggactcgg ccgcgcgctc
      721 ctctgcatct tcctcccgcc gctcgccgtc ctcgacaagg ggtgcgggac gctcctcctc
      781 gtctcgctcc tgacgctcgc cggctgggtg ccgggcgtca tcggcgccct cgtcgtctgc
      841 agccgcgaga aggcctggtg agcgccgccg gggggccgcc tgccgcgcga ggcgcgcgat
      901 gagcgtctcg gtcttcgacc tcttcaagat cggcatcggg ccgagcagct cgcacaccct
      961 cgggccgatg aaggcggcgc ggacgttcgc cgccggcctc gacgcgggag ggctcctcgc
     1021 cgcggtggtc tccgtccggg tgacgctcta cggttcgctc gcggcgacgg cccgcgggca
     1081 cgcgacggat cgcgccgtcc tcctcggcct cctcggcgag aggccggagg agatcgaccc
     1141 cgggaacgtg gacgcgctcg tcgccgccgt gaggcgctcg gggcgtcttg cgctcctcgg
     1201 ccggcacgag gtgccgttcg ccgagggaga ggcgctccgc ctcgagctcg cgccccttcc
     1261 cttccacccg aacggcctcg tcctggaagc gttcggtccg gccggagagc cgctcgcggc
     1321 gaggacgttc ttctcgatcg gcggcgggtt cgtcgtcgag gagggggcgg cggacgcggg
     1381 cgccacggcc gagacggccg tcccgcaccc gtaccggacg gcggaggagc tcctcgccgc
     1441 ctgcgagcgg acggcgctcg ggatcagcgg ggtggtccgg ctgaacgagg gggcgttccg
     1501 gccggaggcc gagacggagg cgggtctcct ccggatctgg gacgtcatgc aggagtgcgt
     1561 ccggcgcggc tgccgcgcgg agggggccct tccgggcggg ctcggggtga agaggcgcgc
     1621 gcccgccctc tttcgcaagc tgacggagtt ccccgagagc ggcctcgccg acccgctcac
     1681 cgcgatcgac tgggtgagcc tctacgccct cgcggtgaac gaggagaacg cggcggggag
     1741 ccgcgtcgtt acggcgccga cgaacggcgc cgcggggatc ctgccggcgg tcctccacta
     1801 ctacaggcgc ttcgtcccgg gcgcgaccga cgcgggcgtc gtgcggatgc tcctgacggc
     1861 cggggcgatc ggcctcctct gcaaggaggg ggcgtcgatc agcggcgccg aggtcggctg
     1921 ccagggagag gtcgggagcg cctgctcgat ggcggccggc gcgctcgccg aggcgctcgg
     1981 cgggacgccg gagcaggcgg cgaacgcggc ggagatcgcc atggagcaca acctcggact
     2041 gacctgcgac ccggtcgggg gcctcgtcca ggtgccgtgt atcgagcgga acgcgatggc
     2101 ggcggtcaag gcgatcaacg ccgcgcgcct cgccctgcgc ggcgacgggc aggccttcgt
     2161 ctccctcgac gacgtcatcc ggacgatgcg cgacaccggg ctcgacatga agagcaagta
     2221 caaggagacg gcgcgcggcg gcctcgcggc ccgcctcgcc gcccacgcga acgtcagcgt
     2281 cggtctcccc gactgctgac ccgggactcc tcccggcgcc cctgctactc tccgccgcca
     2341 tgttcgtcgg ccacttcgga gtcggcttcg cggggaaggc ggcggcgccg cgcgtctcgc
     2401 tcggcacgct cgtcctcgcc tcccagttca tcgacctcct ctggccgacg ctccttctcc
     2461 tcggcctcga gcgggtcgag gtccgtccgg gcatcacccg cgtcacgccg ctcgacttcg
     2521 tctcctaccc gatctcccac tcgctcctgg ccgtcgtcct ctggggagcc ctcttcgccg
     2581 gcgtctacca cctggcgagg cgctccgcgg cgggctccgc cgtcctcttc ggcgcggtcg
     2641 tcagccactg gctcctcgac ctcctgacgc atcgtccgga cctcccgctc ctcccgggga
     2701 gcggcccgaa gatcggcctc ggcctctggg gctccctccc gggtacgctc gtcgtcgagc
     2761 tggccctctt cgccgccggc gtcgcgctct acgtccgcgc gacgcgcccc cgcgatcgcg
     2821 ccgggacctg ggcgctggcc ggcctcgtcg cgttccttct cgtcgtctac gccgcgaacc
     2881 tcttcggcgg cgcgccgccc gacacgaggg cgatcgcctg ggcggggcac gcgcagtggc
     2941 tcctcgtcct ctgggcgtac gcgatcgacc gccacaggat tcccgcggag gcggccggcc
     3001 ctcgcgccat tccggcggcc tgacgcgcct cctgtgaacg ccgcgtcgct cctcggcccg
     3061 ctcgcctgcc tcgggtcggc cgccttctgg gccctcgcgg cgaacgtcta cgcccgcgtg
     3121 gcgcgcgacc acgggccgat ggcggtcaac ttcatgcggg ccgccctttc gttcccgctc
     3181 ttcctggcgg tcgcgctcct cgcccggccg gcgaccggcc tcgccctcgt gacgccgggc
     3241 cgcgcgggat ggctcctcct gagcgtcgtc gggagctacg tcgtgggcga cgcgctgttc
     3301 ctcgcgagcg cccgctccct cggcgtcccg gtctcgctcg cgatcgcctc cacctatccg
     3361 ctctggtcgg ccttcgccgg gtgggcgttc ctcggccaga gcgtcggcgc ggcagggttg
     3421 gccggcgtcg gcctcgtcgt ggcgggagtc gtcacggtga tcggccgggg gcgggggagg
     3481 gacgaggggc cggacgcgtc cgacggcgcc tccgcggggc gcagcgctcg cccgggaacg
     3541 gccggcgtcc tcctcgccct cgccacgtcg ctcttctggg cgctcaacac gttcgcggtg
     3601 acgaaggggg gcgcggacct cccggtcgcg gccgtgaacg tgttccgcat gggcttcgcc
     3661 gcggcgctcc tgccggccgg gacgctcctg atgcggcggc ggccgtcgtc gctcgtcgtc
     3721 cccgccgccg agatgaagcg cgtctggccc gtctttctcc tcgagagcgt cggggggacg
     3781 gcgctcttcg tcgtcgggct gactcacacg ccgctcgcgg tggcggcggc cctgacgtcc
     3841 ctcgcgccgg cgctctccgt gccgttcgcg ctcgtcatcg ggcgcgagcg cctcgacgcg
     3901 cggacgctcg ccgggatctt cgtcgtgacg gcggggatcg tcctcctcgt cgccttcggg
     3961 aggggttgac cgggcgcggc gcggaccgga aggggccggg cgtccccttc cggtccggga
     4021 gactccggcc tcagccccag ggcatcttcg ggagctcgac cttcttgacc ggtcccttga
     4081 tcgccgacgt ggcgcccgtc ccggagtcga cgaggcgcga ctcggtcgtc gaggtgaggc
     4141 gccccttgtc gtccttcgtc acggtcttct cggagaggcg gccgaacgga acggcttcgc
     4201 tcaggcagag ctcggtctcc gtcgtcccct tcatcctcac gatgacgacc ttcacgtcga
     4261 acgagccgcg gatgacgacc ttctctccgg tcttcccgtc ggtcatgcag gggccgaggg
     4321 aggtcacgtc ggccccgtag tcgacgttcg agacgaaggc ggcggcgacc tgccgcatca
     4381 tgtcgggcgg gtactccatc gcctcctcgc cgtcgacctg gaagatcatc gactcgacgt
     4441 acttcatgta gtcgagcgcg ttcttctcga ccttgaagcc ggtgtcgagg aggtacttga
     4501 tcgtcgccgg cttgcccttc gagccttcct tcgggaccga acggatctcg aaccagacgc
     4561 ggtcgccgtc gccgccgagg cgcgacatcg tcatctcgct cttctggacc ttccccttcg
     4621 ggtcggtcga ggtcatctcg tagcgggccc agcttccggg cttcgtcgag gcgaagtagg
     4681 ccttctggag cgtcgtcagg tcggcggcgg gcgcggccga ggccgaggcg agcaggagga
     4741 gcgcggaaag gacggggatc cgtttcatcg atttctctcc tcgggcggga gtgagcccgc
     4801 cccgaccgcg cacgatgcca cgggacgggg ctcgaggggg aacagcgagc cggagtgggc
     4861 cgttctcctc gacgcgcccg tgacgcggcg cacgcctcgc gaggggccgc gtcacggccg
     4921 tcggcgggcc gcatcaggcg cgggggcctc acgccggcgc gcccacacct ccaccgtccc
     4981 tccccggcgc aggaggagct ccgccgtgaa gaggagcccg gagaagggga ggacggccac
     5041 gagcgcgagg agcgtcaggg cgaaggcgaa gggggccgac cggaccgggt gctcgacctc
     5101 gcgcgctccg tctcgcttca gggccgcgta gaggaggttc gccggcaggc cgaggcggtt
     5161 caggaggctc tggatccagc cgtacgggtt ctgctcgagc gcgaagtggc tcacctcctc
     5221 cacgtcgaag ccttcctcgt cgaggagtcg cgcgaggacg gcgagcccga agtggtggta
     5281 gtgccgcggg acgtcgaggt ggaaccagcc gcgcccgccg acgcgcgcct ggaggctctc
     5341 gaggttcggg acggcgacga cgaggaggcc cccgggcgcg aggaggcgcc acgcgcaccg
     5401 gagggccgcg cgcgggtcgc ggagatgctc gaggacgtgc cagaagacga cggccgcgaa
     5461 ggaggcgtcc tcgtacgggc tctcttcgag cgggccgacg aagaccggga tgccgaggac
     5521 ctcgcgggcg tgggtcgcgg cgccctccga gagctcggtc ccgcaggcct cgcgccccag
     5581 ggcgcggagc tgggccggga ggaggccccg gccgcagccg acgtcgagga cgctcccggg
     5641 gcgggcgcgc cggtcgacga ggcgggcgcg ccgcccgcgg aagacgacga tgaggcgctc
     5701 gaggagcggg tggaaacggc gattccgctt tccgtagtac tcgggcgggt agtaggcgcc
     5761 gaccgcctcc tcgtcgagct ccggccgggt cgagacgagg ccgcacgcgg cgcagcgggc
     5821 cagggagaag cgttcgtcga cgtcgaggaa cggacgcatc tccccgctgc agagccggca
     5881 ctccggggcg gccccgggct ccattcgcgc tacgccccgg cgggcttcct ccggctgatc
     5941 aggacgccgc cgacgccgag gagcgtcagg atgagcgcct ggacgagcgt cttgtacgag
     6001 atctccggcc cgccgaagag cgccttccag aggaagaagc ccccgacgac gacgaagagg
     6061 ccgtagaggc cggtcatgat cgaccgggcc ctcgcgttcg cctccggcgt gtcgctcgag
     6121 aagaagcggt tgtagaggag gaggccgacg acggcgacga cgtcgagcgc gacgaagagg
     6181 agccagaacg tccgcgaggc gtcgagcccg gcgggcgtcc cggcgagcgg cttcagccag
     6241 gcgccgtaga gcgcgccgcc gagcgaagag ccgaagaggc tcccgaagac gccgtagagg
     6301 aacgcgtagc ccatgtagac ggccttcttg
//
LOCUS       DAOTEZ010000244         6330 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_8807_length_6330_cov_5.087171, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000244 DAOTEZ010000000
VERSION     DAOTEZ010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6330
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8807_length_6330_cov_5.087171"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="P5164_12835"
     CDS             complement(<1..358)
                     /locus_tag="P5164_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44821.1"
                     /translation="MIKINLLSEAKPAKKKRGVSALGGAGRLNLFLIIGGLLLGLLVV
                     GVQWWVESSRLKEQEEKNRLAQQEVTRLEAVLKEVADFEDKKAKLQKKVDLINSLKAS
                     QRGPVRLMDEVSNALPD"
     gene            complement(372..1427)
                     /gene="pilM"
                     /locus_tag="P5164_12840"
     CDS             complement(372..1427)
                     /gene="pilM"
                     /locus_tag="P5164_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HRY44822.1"
                     /translation="MLFRKAKNLVGLDIGSSAIKLVELRAGKEGQFQLVKAGLESLSP
                     EAIVDGAIMDSSLVVETISRLLASTGIKNPNVAVSVSGHSVIVKKIQLPTMPEEELSE
                     SIRWEAEQYVPFDINDVYLDYVVLDPGAPGETMGVLLVAAKKEKVDDYKNVVTQAGRT
                     PGLVDVDVFALQNCYEVNYGLDPTRVVALVNIGASVMNVNILASGQTVFWRDITFGGN
                     QFTDALQKAFSLNFEQAEALKKGEPVGGQSAQSIQPVLAGVCEDVAGELQKTIDFFHA
                     TSGSEKVDTIVLSGGGSRVGQLDEVLKDRFGLPVEIMNPFRSVVAEDRSVDPAWLSEN
                     GPSLAIGVGLAVRKLGE"
     gene            1682..1876
                     /gene="rpmB"
                     /locus_tag="P5164_12845"
     CDS             1682..1876
                     /gene="rpmB"
                     /locus_tag="P5164_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRY44823.1"
                     /translation="MPQMCEVCGKAPVAGRRVSHAHNVTNRQFKPNLRRVRAAVPGGS
                     KRVTVCTRCLRSDKITKVVR"
     gene            complement(1928..>3778)
                     /locus_tag="P5164_12850"
     CDS             complement(1928..>3778)
                     /locus_tag="P5164_12850"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HRY44824.1"
                     /translation="KEAQQAETFRKMLLAMTGDLRVILVKLADRLHNMRTLHHLREEK
                     RRVIAAETMEIYAPLANRLGMGRIKGELEDLSFRYLHPDEFAALSAAVDERMKASGAA
                     IESLRVDIGARLDEAGVPADVTGRVKRLWSIRQKLIRQSIPLDQLYDVLAFRVLVDSV
                     RDCYSALGIVHQAWRPVPGRIKDYIAMPKQNFYQSLHTTVIPEGEPPFEIQIRTREMD
                     LVAEQGIAAHWKYKEGRGSRKVDEEGIAAIRQILETTRDIANPREFLSSLKMDLYADE
                     VYTFTPKGAVYSFPRGATPVDFAYRIHTDVGHRCVGARVNGRLVPLRTPLVNGDIVEI
                     LTAPSGAPSRDWLAFVVTSRARNKIRAFLQTKEKQKSVEIGRRFLEKELKKVKRSLGK
                     LVDAKALDGILADFGVPRVDDLLAEIGYGKVPPRAVVQKLFPQDEPAADRTPSRTDAL
                     RKVVRKILPLGGTGILVRGENDLLASLAKCCRPVPGEEIVGYVTRGRGVSVHAAACPN
                     VRNLLFDPSREIEVEWHVGKDASFTVDFVVEVEDRPGILARITQVISDAESNIESIEA
                     RTSPGGTALIEGSVTTPGRRHLDKLFLAIRSVPGVTGVRRKFNVSRIGSR"
     assembly_gap    3779..3878
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3879..4263)
                     /locus_tag="P5164_12855"
     CDS             complement(<3879..4263)
                     /locus_tag="P5164_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRY44825.1"
                     /translation="MLRFEDILDAVESYLPGADEDLLRRAYIFSAQAHRHQLRSSGEP
                     YLVHPIGVAMLLAELKLDEVSVATGLLHDVLEDTKTTKEELASLFGSEVAEVVDGVTK
                     IGRYAYTSKEAQQAETFRKMLLAMTG"
     gene            4329..5015
                     /locus_tag="P5164_12860"
     CDS             4329..5015
                     /locus_tag="P5164_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018046265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HRY44826.1"
                     /translation="MSMPAPAPETAPAPEPTTPAAPAHPLLRALAAPRRWIRGLYDWT
                     MRWADKPHALAALFLIAVVESSVFPIPPDVLLIAIVAASPRLWVRSAALCTIGSVIGA
                     GGGYLIGTAFMATLGQRIVEFYNAQHHWAKVVELYNGEWGIWFLAAAAFTPIPYKVAT
                     IAAGATGMAFVPFLLVSILGRGARFFLVAGLLRLFGPAIRRTLEKNFDLAALAFLVLL
                     VGGFLALKYF"
     gene            complement(5074..6093)
                     /locus_tag="P5164_12865"
     CDS             complement(5074..6093)
                     /locus_tag="P5164_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRY44827.1"
                     /translation="MTSVSLFPFAETWWVYGLFVVFVVGMLALDLGVFHRKAHVVGFR
                     EAAAWSAVWVVVAMGVGTVLYFWASARFGADARLLAVPGFVPDGAARQVFLEFLTGYV
                     IEKALSVDNIFVFVVLFQFFGVPAAYQHRVLYWGILGALVFRAIFIALGALLLQFHWV
                     MYVFGAFLVITGVKLVFGPDKPVDPADNAVLKALKRWMPLTHELHGPHLFAREGGRLM
                     GTPLLLALVFIEISDVVFAIDSVPAIFAITREPLLVFTSNICAILGLRALFFLLAGAV
                     GKFHLLKYGLGLVLVFVGLKMAWLNDAFGGHFPIGLSLGIIGALLAASIVLSLVFPPR
                     AEREG"
BASE COUNT          952 a         2147 c         2142 g          989 t
ORIGIN      
        1 ggtccggcag ggcattggag acctcgtcca tcagacgcac cggaccgcgc tggctcgcct
       61 tcagggaatt gatcaggtcg accttcttct ggagcttcgc cttcttgtcc tcgaagtccg
      121 cgacttcctt cagcaccgcc tcgaggcggg tgacttcctg ctgagcgagg cggttcttct
      181 cctcctgctc cttgaggcgc gaggattcga cccaccactg gacgccgacg acgagcagtc
      241 cgagcaggag ccctccgatg atcaggaaca ggttcagccg gccagctccg ccgagggcgg
      301 aaacgccccg tttctttttt gcgggcttgg cctcggagag gaggttgatc ttgatcatct
      361 ggcctcctcc gtcactcgcc gagcttccgc acggcgagcc cgacgccgat cgccagcgac
      421 gggccgttct ccgagagcca ggccgggtcg acggagcggt cctcggcgac gacggagcgg
      481 aacggattca tgatctcgac gggcaggccg aagcggtcct tgaggacttc gtcgagctgc
      541 ccgacccgcg agcctccgcc ggagaggacg atcgtgtcga ccttctccga gccggaggtc
      601 gcgtggaaga agtcgatcgt cttctggagc tcgccggcga cgtcctcgca gacgcccgcg
      661 aggacgggct ggatgctctg cgcggactgc cctccgaccg gctctccctt cttgagcgcc
      721 tcggcctgct cgaagttgag ggagaaggcc ttctggagcg cgtccgtgaa ctggttccct
      781 ccgaacgtga tgtcgcgcca gaagaccgtc tggccgctcg cgaggatgtt cacgttcatc
      841 accgacgcgc cgatgttcac gagcgcgacc acgcgcgtcg ggtcgaggcc gtagttgacc
      901 tcgtagcagt tctggagggc gaagacgtcg acgtcgacga ggccgggagt ccgccccgcc
      961 tgcgtgacga cgttcttgta gtcgtccacc ttctccttct tcgccgcgac gagaaggacc
     1021 cccatcgtct ccccgggagc acccgggtcg aggacgacgt agtcgaggta gacgtcgttg
     1081 atgtcgaacg gaacgtactg ctccgcctcc caccggatcg actcggagag ctcctcctcc
     1141 ggcatcgtcg ggagctggat cttcttgacg atgacggagt ggcccgagac ggagacggcg
     1201 acgttcgggt tcttgatacc ggtcgatgcg agcaggcggg agatcgtctc gacgacgagc
     1261 gaggaatcca tgatggcgcc gtccacgatc gcttccggcg agagactctc cagcccggcc
     1321 ttcacgagct ggaactgccc ttccttccct gcccggagct cgaccagctt gatggcggac
     1381 gagcctatgt cgagaccgac gaggttcttg gccttgcgga acaacacggt gcggtctccc
     1441 tccctctacg ttccggaacc gacgcgacac cctgacacga gctgggtaaa gcctttgtgg
     1501 tggctatgtt gcgcgggtcg tccgagactg tcaaggacga gtaaggcccg aaatgcctct
     1561 ggacaccgag gccgaggggc gttataagtc gcagttccgg cggcgactcc ggcccgctcc
     1621 gacgaggacg gtcccggggc gccgccgggc atcgcgttca ggaatcagag ggagtccccc
     1681 catgccccag atgtgtgaag tctgcggaaa ggccccggtc gccggtcgcc gggtcagcca
     1741 cgcccacaac gtcacgaacc ggcagttcaa gccgaacctc aggagggtgc gcgcggcggt
     1801 tcccggcggc tcgaagaggg tcacggtctg cacccgctgc ctccggtccg acaagatcac
     1861 gaaggtcgtc cgctgacccc gccgcccgcc gcggggaggg cgctccccgc cggcgaggcc
     1921 cggtctctca gcgcgacccg atccgggaga cgttgaactt ccgtctcact ccggtgactc
     1981 ccgggacgga ccggatcgcg aggaagagct tgtcgaggtg gcggcgcccc ggcgtcgtga
     2041 cgcttccttc gatcagagcc gtcccgccgg ggctcgtccg ggcctcgatc gactcgatgt
     2101 tcgactccgc gtccgagatg acctgcgtga tcctcgccag gatccccggt cggtcctcga
     2161 cctcgaccac gaagtcgacc gtgaacgatg cgtccttccc gacgtgccac tcgacctcga
     2221 tctcccggct cgggtcgaag aggaggttgc gcacgttcgg gcaggccgcg gcgtggaccg
     2281 acaccccgcg cccccgcgtc acgtagccga cgatctcctc cccgggcacg ggacggcagc
     2341 acttcgcgag gctcgcgagg aggtcgttct cgccgcggac gaggatgccc gtcccgccga
     2401 gcgggaggat cttccggacg accttcctga gggcgtccgt ccgcgacggc gtcctgtcgg
     2461 ccgcgggctc gtcctgcggg aagagcttct ggacgaccgc gcgggggggg accttcccgt
     2521 agccgatctc cgcgaggagg tcgtcgacgc gcgggacgcc gaagtcggcc aggattccgt
     2581 cgagcgcctt ggcgtcgacg agcttcccca gcgaacgctt gaccttcttg agctccttct
     2641 cgaggaagcg ccgcccgatc tcgaccgact tctgcttctc cttcgtctgg aggaacgccc
     2701 ggatcttgtt ccgggcgcgc gacgtcacga cgaacgcgag ccagtcgcgc gacggggcgc
     2761 ccgacggcgc cgtgaggatc tcgacgatgt cgccgttcac gagcggggtc cggagcggga
     2821 cgagccggcc gttcacccgg gcgccgacgc agcggtgccc gacgtcggtg tggatccggt
     2881 aggcgaagtc gaccggcgtc gcgccgcgcg ggaatgagta gacggccccc ttcggcgtga
     2941 acgtgtagac ctcgtcggcg tagaggtcca tcttcagcga ggaaaggaac tccctcgggt
     3001 tcgcgatgtc gcgcgtcgtc tcgaggatct ggcggatcgc cgcgatcccc tcctcgtcga
     3061 ccttccggct cccgcgcccc tccttgtact tccagtgcgc cgcgatcccc tgctccgcga
     3121 cgaggtccat ctcgcgcgtc cggatctgga tctcgaacgg cggctcccct tccgggatca
     3181 ccgtcgtgtg gagcgactgg tagaagttct gcttcggcat cgcgatgtag tccttgatcc
     3241 ggcccgggac gggccgccag gcctggtgga cgatgccgag ggcgctgtag cagtcgcgga
     3301 ccgagtcgac caggacgcgg aacgcgagga cgtcgtagag ctggtcgagc gggatcgact
     3361 gacggatcag cttctggcgg atggaccaga ggcgcttcac gcggccggtc acgtccgcgg
     3421 ggactcccgc ctcgtccagg cgggcgccga tgtcgacgcg gaggctctcg atcgcggcgc
     3481 ccgacgcctt catccgctcg tcgaccgccg ccgagagcgc cgcgaactcg tccgggtgga
     3541 ggtatcggaa cgagaggtcc tcgagctccc ccttgatccg gcccatcccg agccggttcg
     3601 cgagcggggc gtagatctcc atcgtctcgg ccgcgatcac gcgccgcttc tcctcgcgga
     3661 ggtggtggag cgtccgcatg ttgtggagcc ggtcggcgag cttgacgagg atgacgcgga
     3721 ggtcccccgt catcgcgagg agcatcttcc ggaacgtctc ggcctgctgc gcctccttnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc cccgtcatcg cgaggagcat
     3901 cttccggaac gtctcggcct gctgcgcctc cttcgaggtg taggcgtagc ggccgatctt
     3961 cgtgaccccg tccacgacct cggctacctc gctcccgaag agcgaggcca gctcctcctt
     4021 cgtcgtcttc gtgtcctcga ggacgtcgtg gaggaggccg gtggccacgg agacctcgtc
     4081 gagcttcagc tccgcgagga gcatcgcgac cccgatcggg tggacgaggt acggctctcc
     4141 cgaggagcgg agctggtgcc ggtgcgcctg ggccgagaag atgtacgccc tccggaggag
     4201 gtcctcgtcg gcccccggaa ggtagctctc gaccgcgtcg aggatgtcct cgaacctcag
     4261 cacggactga ttatggcgtc cccgcctccg cgctgcgcgc caggcggggt tcgggtaacc
     4321 tccgccggat gtcgatgccg gcccccgcgc ccgagaccgc cccggccccc gagccgacga
     4381 cgcccgccgc tcccgcccat cccctgctgc gggccctcgc cgcgccgcgc cggtggatcc
     4441 gggggctcta cgactggacg atgcgctggg ccgacaagcc ccacgcgctg gcggccctct
     4501 tcctgatcgc cgtcgtcgag tcgtcggtct tcccgatccc gcccgacgtc ctcctcatcg
     4561 cgatcgtcgc ggcctcgccc cgcctctggg tccgctccgc ggcgctctgc acgatcggat
     4621 cggtcatcgg cgccggcggc gggtacctca tcgggaccgc gttcatggcg acgctcggcc
     4681 agcggatcgt cgagttctac aacgcccagc accactgggc gaaggtcgtc gagctctaca
     4741 acggtgaatg ggggatctgg ttcctcgcgg ccgccgcgtt cacgccgatc ccctacaagg
     4801 tcgccacgat cgcggccggg gcgaccggga tggccttcgt accgttcctg ctcgtgagca
     4861 tcctcggccg cggagcgcgc ttcttcctcg tggccgggct gctccggctc ttcggccccg
     4921 cgatccgccg gaccctcgag aagaacttcg atctcgcggc gctcgcgttc ctcgtcctcc
     4981 tcgtcggggg cttcctcgcg ctgaagtact tctgatccgg accggcctct cgacgcgctc
     5041 gggcccgcgg gcccggctgc gcagcccgtc ctcctatccc tcgcgctcgg cgcggggcgg
     5101 gaagacgagc gagaggacga tcgaggcggc gaggagcgcg ccgatgatcc cgagcgaaag
     5161 gccgatcggg aagtgcccgc cgaaggcgtc gttcagccac gccatcttca gaccgacgaa
     5221 gacgaggacc aggccgaggc cgtacttcag caggtggaac ttgccgaccg cgcccgcgag
     5281 gaggaagaag agggcgcgca ggccgaggat cgcgcagatg ttcgacgtga agacgaggag
     5341 cggctcgcgc gtgatcgcga agatcgccgg gacggagtcg atcgcgaaga cgacgtcgga
     5401 gatctcgatg aagacgaggg cgaggaggag cggcgtcccc atcagccgcc ccccctcgcg
     5461 ggcgaagagg tgcggcccgt ggagctcgtg cgtgagcggc atccagcgct tcagcgcctt
     5521 caggaccgcg ttgtccgccg ggtcgaccgg cttgtccggc ccgaagacga gcttgacccc
     5581 ggtgatcacg aggaatgccc cgaagacgta catgacccag tggaactgga ggaggagcgc
     5641 gccgagcgcg atgaagatcg cccggaagac gagcgcgccg aggatccccc agtagaggac
     5701 ccggtgctgg taggcggccg ggacgccgaa gaactggaag aggacgacga agacgaagat
     5761 gttgtcgacc gagagcgcct tctcgatgac gtagcccgtc aggaactcga ggaagacctg
     5821 ccgggccgcc ccgtcaggga cgaagcccgg gacggccagg aggcgcgcgt cggccccgaa
     5881 gcgggcggag gcccagaaat agaggaccgt tccgacgccc atcgccacga cgacccagac
     5941 ggcgctccag gcggcggcct cgcggaaccc gacgacgtgc gccttcctgt ggaagacgcc
     6001 gaggtcgagg gcgagcatcc cgacgacgaa gacgacgaag aggccgtaga cccaccaggt
     6061 ctcggcgaag gggaagaggg agacgctcgt catcggagtc ctctcgggcg gccggggaga
     6121 ccgggcgccg gggcgccctc atcgtggccg cgccgcccgg cgcggccacg atgggagaga
     6181 gggtacggcc tcagcggccc ttcgcggccg ccttgcccgc cttgcccgcg tccgggacgg
     6241 aggcggggtg ggcggcggcg cgcttcgcct tccaggtctc ccagttcacg acccaggggg
     6301 ccgcgatgaa gatcgtggag taggcgccga
//
LOCUS       DAOTEZ010000245         6291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_8883_length_6291_cov_5.859365, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000245 DAOTEZ010000000
VERSION     DAOTEZ010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6291
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8883_length_6291_cov_5.859365"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="P5164_12870"
     CDS             <1..321
                     /locus_tag="P5164_12870"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRY44828.1"
                     /translation="FSRQLLAGFGLEELFAYVGGPDLGFPEKPDPAMVRAAIGAMGVP
                     PAETLFVGDMVVDVATARAIGLAVAVVPLGSSTREELLAERPDYLFGSLAEVLGLFGE
                     VRAV"
     gene            444..3860
                     /locus_tag="P5164_12875"
     CDS             444..3860
                     /locus_tag="P5164_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44829.1"
                     /translation="MARTLSRVCRSAILVLGPLFALPSAAELVWKVADEGPRAPGSVG
                     IVGDDSRVLASGSGGTWELDGTTWRRISLDGSASPEGNLAPSFVAGGRFIAYTVRDRG
                     IQVYVLRENAWVPLGEVPDAGYDHVPSDDRLFVSTIRFNDCEPDPAGVHEAEGRRLRS
                     ISFADGSIREEPPLPARNGTFFVLFGKLHLLEAGGASGGCAGPSSGLAGLAAAAQAPI
                     PFHRLDGDHWTTLEPWPALGWGFRSTPNSLWGFYSESPTGVVVRVLTAAGLSGPIPDP
                     QGAHNVFYGARPFEWNGRLIVATRTARGTIYELKNGRFEVLAPESPVLGERSLVPNVT
                     VAGRRLLTWARGWDVHVREAEAWVPTVGAGETIGADTYFASASRRFATLGNRVFRRGE
                     AGWSRLAPLPITGSITGAVAVGEKAIVAVDDGEDHLFEHDPASDSWRDLKLPEGFGLF
                     DLGLPNGLQRSLAATADAVWVNGTGNRLARCREGAWTIFNPPQPLPLGTSRSVVRVLD
                     GTPHLFGWRGETRPSAFVLRNERLEEAFPGLDPTLRVLDAASAGGRTFLAVRGSNGDD
                     LAAALVSFDGTEFRTLLTASDLRNANLSSDATAPIAAVGDTAFFAQLAFTDGRLRRPM
                     GPLAPRAIDPSGAFGFDGARRYERDHPFLVPVPRVRKHLASVVDVFGVWGIRYRSSLS
                     VANLSSSRPAVARLFPGTAVVPARELPLAPGAQLRLEDPFPGFVGPVTVEFEGLEDES
                     EAWAAVRVWSPSEGGTAGTSVVATDPGGIGADGTTLRPVTTPDSRLHVAMAATNDGTG
                     AGLWVWDPRWAEPWVDSALTRVQRGGFLQVDPHPEAVARPLVLAPSLITDSTGWTDEP
                     IDLLGYLVRNDGATNDGTVVPFEPPDAIPGRLTRFLPAVVGVTSAHAAYRTELTLGWR
                     STDGERPSRLEFQATYRDDAVSKSFPVALDGRPSSGGMPFGEVLEVPDAGAWLVANGV
                     PVDPQAFVGTLSFTSERPEGAAALLVTAVVQARAPGAKGDYGVSVPVVNEGRWAKERA
                     IVPGLREDGSFRSNVALANPEGSGGPEVTLEVTLHAAPDGAVIGTLPRVTLAPGRRVQ
                     LDRPLGQVGYGGDAWAEVRRVGGAGRFVAYGVVNDAVTSDGTLFPMAGAK"
     gene            3953..5080
                     /locus_tag="P5164_12880"
     CDS             3953..5080
                     /locus_tag="P5164_12880"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HRY44830.1"
                     /translation="MSSSAFDSTSGHAGQVRFFNPGPVWVRPQVLQALGGPMMSHRSA
                     AFSDLYGRILDKLPKVFRTKGQAHTLATSATGVWEAGLVSCATGPVLACCNGSFSDKW
                     GEMSQRLGLETDFVRSDWGKPILADDVKKALAAKKYQVVTVVHNETSVGTISDLAAIS
                     KVIRENSDALVFADVVTSLAATQVETDEWGLDLAVCGSQKALALPPGIAVFAVSEKAL
                     AAARAKKCRGLYLDLLDVDAFGKKKQNPTTPSLPLLYALDVQLDHILAEGMEARWARH
                     HEMLKAVEEWAPKAGFRFLPDPPAHSPSVSSLFPPEGVGAEDLNKAMKGAGWVPGSGY
                     GKFKSLNIRIGHMGDVDVASVKNVLAAYEEQAAKLLAASKG"
     gene            5228..>6291
                     /locus_tag="P5164_12885"
     CDS             5228..>6291
                     /locus_tag="P5164_12885"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HRY44831.1"
                     /translation="MSIRTRVGVVFGGPSREHGVSFLSARCLLENLPSDRFEAVPVFV
                     GKDGRWHGVADSREEMLRYFARPAREALLDGVVPGSRPAPPSEIVASFEAAFRDGAWG
                     VVFPILHGTFGEDGVLQGLLEAMNVPYVGFGVAASAVGMDKLLMKATFGAAGLPQVRY
                     VGIRAAAWRDTAHRAALLARTEALGLPLFVKPACAGSSVGITKVKERAGLEAAVDAAL
                     AVDATALVEAAADAREVECAVLEGERGEPEASFPGEVVPGHEFYDYEDKYIDDRSYSI
                     LPAELPEETSNEVRRMACAAFDAVGGAGFARVDFFVERASGRVLLNEINSLPGFTPIS
                     MFPKMWEATGLPLPQLLDRLV"
BASE COUNT          799 a         2294 c         2238 g          960 t
ORIGIN      
        1 ttcagccgtc agctcctcgc cggcttcggc ctcgaggagc tcttcgccta cgtcggcggg
       61 ccggacctcg gcttccccga gaagcccgac ccggcgatgg ttcgggccgc gatcggcgcg
      121 atgggcgtgc cgcctgccga gacgctcttc gtcggcgaca tggtcgtcga cgtcgcgacg
      181 gcccgcgcga tcggtctcgc cgtcgccgtc gtcccgctcg gctcctcgac ccgcgaggag
      241 ctcctcgcgg agcggcccga ctacctcttc ggctccctcg cggaggtgct cgggctcttc
      301 ggggaggtcc gcgccgtctg agaccgttgc gccggccgag gcgctcgaag gtccgttgcg
      361 ggccggtcgg cgcggtggag cgggtgcact ctcccgcgtg aggtggcggc gtcgcggcac
      421 acttccttca tcgggggatg acgatggctc gaacgctctc tcgtgtttgc cgctccgcga
      481 tcctggtcct cggtccgctc ttcgcccttc cgtccgccgc cgaactcgtc tggaaggtcg
      541 cggacgaggg gccgcgggcg ccgggctcgg tcgggatcgt cggcgacgat tcgcgcgtcc
      601 tcgcgtccgg ctcgggcggg acgtgggagc tcgacgggac gacctggcgg cgaatctcgc
      661 tcgacggctc ggcctcgccg gaggggaatc tcgccccgtc tttcgtcgcg ggcgggcgat
      721 tcatcgccta caccgttcgg gatcgaggca ttcaggtgta cgtcctgcgc gagaacgcct
      781 gggtcccgct cggggaggtg cccgacgccg ggtacgacca cgtcccgtcg gacgaccggc
      841 tcttcgtctc cacgatccga ttcaacgact gcgagccgga cccggcgggc gtccacgagg
      901 ccgagggccg gcggctccga tcgatctcgt tcgccgacgg gtcgatccgc gaggagccgc
      961 cgctccccgc ccggaacggt acgttcttcg tcctcttcgg caagctccac ctcctggagg
     1021 cgggaggggc gtcgggcggt tgcgcaggcc cgagctcggg ccttgcgggc ctcgccgcgg
     1081 cggcgcaggc cccgatcccg tttcatcggc tggacggcga tcactggacg acgctcgagc
     1141 cctggccggc ccttggctgg ggcttccgct ccacgccgaa cagcctctgg ggcttctatt
     1201 ccgagagccc gacgggcgtc gtggtgcgag tcctcacggc ggccggcctc tccgggccga
     1261 tccccgatcc gcagggcgcc cacaacgtct tctacggcgc tcgtccgttc gagtggaacg
     1321 gccggctcat cgtcgcgacc cggacggcgc gcgggacgat ctacgagctg aagaacgggc
     1381 gcttcgaggt gctggcgccg gagtctccgg tgctcggaga gcggtccctc gtcccgaacg
     1441 taacggtcgc cggacgacgg ctcctcacct gggcgcgcgg gtgggatgtc cacgttcgcg
     1501 aggcggaagc ctgggtgccg acggtgggcg cgggcgagac gatcggggca gacacgtact
     1561 tcgcgagcgc ctcgaggcgt ttcgcgacgc tcgggaaccg cgtcttccgg cgaggcgagg
     1621 cgggctggtc ccggcttgcc ccgctcccga tcactggctc gatcacgggc gccgtcgcgg
     1681 tcggcgagaa ggcgatcgtc gccgtcgacg acggggaaga ccacctcttc gagcacgacc
     1741 cggcctccga ctcgtggcgc gacctgaagc tgcccgaggg cttcggcctc tttgacctgg
     1801 gccttccgaa cggcctccag cgttccctgg cggcgacggc ggatgcggtc tgggtgaacg
     1861 ggaccgggaa ccgtctcgcg cgctgccgcg agggcgcctg gacgatcttc aacccgccgc
     1921 agccccttcc cctggggacc tcccgaagcg tcgtccgcgt cctggacggc accccgcacc
     1981 tcttcgggtg gcggggcgag acccggccct cggccttcgt ccttcggaac gagcgcctcg
     2041 aagaagcgtt cccgggcctc gacccgacgc ttcgcgtcct cgacgcggcg tcggccggag
     2101 gtcgcacctt cctggccgtc cggggatcga acggcgacga ccttgcggca gcgctcgtct
     2161 cgttcgacgg caccgagttc cggacgctcc tcacggcgag tgacctgcgg aacgcgaatc
     2221 tgagctccga cgcgacggcg ccgatcgccg ccgtcggcga cacggcgttc ttcgcgcagc
     2281 tcgcctttac cgacggccgc ctgcgccgcc cgatgggtcc gctcgcgccc cgcgcgatcg
     2341 acccctcggg ggcgttcggc ttcgacggcg cgcggcgcta cgagagggac caccctttcc
     2401 tcgtcccggt gccgagggtc cgcaagcacc tcgcctccgt cgtcgacgtg ttcggggtct
     2461 gggggatccg ctaccggagc agcctctccg tcgcgaacct ctcgtcgtcc cgtcccgccg
     2521 tcgcccggct cttcccggga acggcggtcg tccccgcgcg cgagcttccg ctcgcgccgg
     2581 gtgcgcagct ccgcctcgaa gaccccttcc cgggattcgt cggtccggtc accgtcgagt
     2641 tcgagggcct cgaggacgaa tcggaggcct gggcggccgt gcgggtctgg agcccctcgg
     2701 aaggggggac ggcggggacc tccgttgtcg cgaccgatcc cggcggcatc ggcgccgacg
     2761 ggacgacgct tcggcccgtg acgactcccg acagccggct ccacgtcgcg atggcggcga
     2821 cgaacgacgg gacgggcgcg ggcctctggg tctgggaccc gaggtgggcc gagccgtggg
     2881 tcgacagcgc cctcacgagg gtgcagagag gagggttcct tcaggtcgac ccccaccccg
     2941 aggccgtcgc gcggccgctc gtcctcgctc cgagcctcat cacggattcg acaggctgga
     3001 ccgacgagcc gatcgatctc ctgggatacc tcgtccggaa cgacggagcg acgaacgacg
     3061 gaaccgtcgt cccgttcgag ccgccggacg cgatcccggg ccgcctcacg cggttcctgc
     3121 cggccgtcgt cggcgtcacc tccgcacacg ccgcctacag gacggagctg acgctcggct
     3181 ggcgatcgac ggacggagag cgcccctccc ggctggagtt ccaggccacc taccgcgacg
     3241 acgccgtctc gaagtcattc ccggtcgccc tcgacgggag gccgtcgtcc ggcgggatgc
     3301 cgttcggcga ggtcctcgag gtccccgacg ccggcgcgtg gctcgtcgcg aacggcgtgc
     3361 cggtcgaccc tcaggccttc gtcggcacgc tctccttcac gtcggaacgc ccggaggggg
     3421 ccgccgcgct cctcgtgacc gccgtcgtcc aggcgcgggc cccgggcgca aagggggact
     3481 acggcgtcag cgtcccggtc gtgaacgagg ggcgctgggc gaaggagcgg gcgatcgtcc
     3541 ccggcctgcg cgaggacggt tcgttccgct cgaacgtcgc tctcgcgaac cccgaaggct
     3601 cgggcgggcc ggaggtgacg ctcgaggtga cgctgcacgc cgcgccggac ggagcggtga
     3661 tcggaacgct gccgcgcgtc acgctcgcgc cgggccggcg ggtccagctg gaccggcctc
     3721 tcggccaggt cggctacggc ggcgacgcct gggccgaagt gcgccgggtc ggcggcgcgg
     3781 ggcgcttcgt cgcctacggc gtcgtgaatg acgccgtgac gtccgacgga acgctcttcc
     3841 caatggcggg cgcgaagtga gcggaaatgg actggtcggc ccggctccgc cgttggctca
     3901 cgaagggctt cgcgatgctt cgagcgcgca ccccgagggt aaaatcctga acatgagcag
     3961 ctccgccttc gactccacct ccggccacgc cgggcaggtt cgtttcttca accccggccc
     4021 cgtgtgggtc cgtccgcagg tgctccaggc cctcggcggc ccgatgatgt cccaccgttc
     4081 cgcggccttc tccgacctct acggccgcat cctcgacaag ctcccgaagg tcttccggac
     4141 gaaggggcag gcccacacgc tcgccacgtc cgcgaccgga gtctgggagg ccggcctcgt
     4201 ctcctgcgcc acgggtcccg tcctcgcctg ctgcaacggc tccttctccg acaagtgggg
     4261 cgagatgagc cagcggctcg gcctcgagac cgacttcgtc cggtccgact ggggcaagcc
     4321 gatcctcgcc gacgacgtga agaaggcgct cgcggcgaag aagtaccagg tcgtcacggt
     4381 cgtccacaac gagacctccg tcgggacgat ctccgacctc gccgcgatct cgaaggtgat
     4441 ccgcgagaac agcgacgcgc tcgtcttcgc cgacgtcgtg acctccctcg ccgcgactca
     4501 ggtcgagacg gacgagtggg gcctcgacct cgccgtctgc ggctcgcaga aggcgctcgc
     4561 gcttcctccc ggcatcgccg tcttcgccgt gtcggagaag gcgctcgcgg cggcccgcgc
     4621 gaagaagtgc cgcggcctct acctcgacct cctcgacgtc gatgcgttcg gcaagaagaa
     4681 gcagaacccg acgacgccgt cgctcccgct cctctacgcc ctcgacgtcc agctcgacca
     4741 catcctcgcc gaggggatgg aggcccgctg ggcgcgccac cacgagatgc tgaaggcggt
     4801 cgaggagtgg gcgccgaagg ccggcttccg cttcctcccc gacccgccgg cgcattcgcc
     4861 gagcgtctca tccctcttcc cgcccgaggg cgtcggcgcg gaggacctga acaaggcgat
     4921 gaagggcgcc ggctgggtgc cgggctcggg ctacggcaag ttcaagtcgc tcaacatccg
     4981 gatcggacac atgggcgacg tcgatgtcgc gtcggtgaag aacgtcctcg ccgcctacga
     5041 ggagcaggcc gcgaagctcc tcgcggcgtc gaagggctga acggcagggc gccggagcct
     5101 ggccgatcga gacgacgctg cgggccccgg ggtcgtgacg accccggggc ccgttctcgt
     5161 ttgcggtacg ctgccgggaa ggacttaagg cctcctctga ggaggaggcg acgaacaggg
     5221 agcgcgcatg agcatccgca cccgggtcgg agtcgtcttc ggagggccgt cgcgggagca
     5281 cggcgtctcc ttcctctcgg cgcgctgcct gctcgagaac ctcccgtccg accgattcga
     5341 ggccgtgccg gtcttcgtcg gcaaggacgg ccgctggcac ggcgtcgccg attcgcgcga
     5401 ggagatgctt cggtacttcg cgcgtccggc gcgggaggcg ctcctcgacg gcgtcgtccc
     5461 cggctcgcga cccgcgcctc cgtccgagat cgtcgcgtcg ttcgaggcgg cgttccgcga
     5521 cggggcctgg ggcgtcgtct tcccgatcct ccacggcacg ttcggcgagg acggcgtcct
     5581 gcaggggctg ctggaggcga tgaacgtccc gtacgtcggc ttcggcgtcg ccgcctcggc
     5641 cgtcgggatg gacaagctcc tgatgaaggc gacgttcggc gcggccggcc tgccgcaggt
     5701 ccgctacgtc ggaatccgcg cggcggcctg gcgcgacacg gcgcaccgcg cggcgctcct
     5761 cgcccggacg gaggccctcg gcctcccgct cttcgtgaag ccggcctgcg ccggctcttc
     5821 cgtcgggatc acgaaggtca aggagagggc cgggctcgag gcggccgtcg acgcggccct
     5881 cgcggtcgac gcgacggccc tcgtcgaggc ggcggccgac gcccgagagg tcgagtgcgc
     5941 cgtcctcgaa ggggagcggg gcgagcccga ggcctcgttc cccggcgagg tcgtgccggg
     6001 gcacgagttc tacgactacg aggacaagta catcgacgac cgctcctatt cgatcctccc
     6061 ggcggagctg ccggaggaga cctcgaacga ggtccgacga atggcctgcg ccgcgttcga
     6121 cgccgtcggg ggcgccgggt tcgcgcgcgt cgacttcttc gtcgagcgcg cctcggggcg
     6181 cgttctcctg aacgagatca actcgctccc cggcttcacc ccgatctcga tgttcccgaa
     6241 gatgtgggag gcgacggggc tgccgctccc gcagctcctc gaccggctcg t
//
LOCUS       DAOTEZ010000246         6283 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_8906_length_6283_cov_6.066313, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000246 DAOTEZ010000000
VERSION     DAOTEZ010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6283
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8906_length_6283_cov_6.066313"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..1558)
                     /locus_tag="P5164_12890"
     CDS             complement(236..1558)
                     /locus_tag="P5164_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004067418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HRY44832.1"
                     /translation="MAKGVRRLAIIGAAGRDFHNFNTTYRDDPSVEVVAFTATQIPGI
                     AGRRYPVELAGSRYPQGIRIVPEDDLERLIRDERIDACVFSYSDVSHETVMHLASRCI
                     VAGADFELLSNDRTMIPSMAPVVAITAVRTGAGKSQTTRYVSSILKKLGKKVVAIRHP
                     MPYGDLAAQACQRFASYADLDHHKCTIEEREEYEPHIDNGFIIYSGVDYGQILKAAES
                     EAEVILWDGGNNDLPFYRPDLHICIADPLRPGHEMLFHPGEANFRRADVIVINKCDSA
                     EAKAIEAIEAVAAKVNPKARVLRANSPATCEKPELVKGKRVLVIEDGPTLTHGGMTYG
                     AGVVAAKKLGAAEIVDPTPYAVGSIKATYEKYPNAEGILPAMGYSPLQIAELEATIEK
                     TPCDAVVSGTPIDLTRVLKIGKPATRVSYELEPLVPGLLEEEVRKILR"
     gene            complement(1634..2356)
                     /locus_tag="P5164_12895"
     CDS             complement(1634..2356)
                     /locus_tag="P5164_12895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY44833.1"
                     /translation="MTSHELRTPLNAILGYSELLMEEISERRLPGLAEDVEKVHSAGR
                     HLLGLINDLLDLSRLEAGKLELSVEETELAPLLAGVVTTMRPLVERNRNRFVVEGADG
                     AGRLVTDPTRLRQVLLNLLANAGKFTHDGEVAFRVQRLAGPEGGRVRFSVTDTGIGMT
                     EEQVGRLFRAFEQADPQVARRFGGTGLGLVITRRLARMMGGDVFVESRPGEGSTFVVE
                     LPERPAEAGPNPERESGSQDRM"
     gene            complement(2353..4851)
                     /locus_tag="P5164_12900"
     CDS             complement(2353..4851)
                     /locus_tag="P5164_12900"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRY44834.1"
                     /translation="MAPGPRALAPGILAILLTAAPAEALDPGKAISQYRHEVWGVREG
                     LPQSSAEALAQTRDGYLWIGTQEGLARFDGVRFVVWDKASAPELRHNRVVALLEDRSG
                     RLWLGTEGGGLSVLQDGTIRTFGRSEGLGSDIVRSLAEDGAGTLWIGTETGLAARAGG
                     SFETREASSGLPGGAVRAVAVDEAGVVWAGTDRGLFRRSGRRFVTTGVTDAVQSIHAG
                     ADGTLVVGTAHGLRLLREGRVEALGRADGLPGEVVNCALRDRRGTLWIGTSGGLARWA
                     GGRLSAFTLREGLPAPNVLALLEDREGTLWVGTQDGGLNKLSDASFTPWGAAEGLSAD
                     IAWAVFVDRDGAVWSGTDSAGVNVRRGDRVTTIGTAEGLAAPGVQAIWQAPDGAVWLG
                     TRGGGVSIVRQGRVVRTLRRADGLASDSICAITGTRDGSVFLGGRGGGLTRIGPDGTY
                     RRWGAGEGLRNETVHALLPDRDGNLWIGTNGGGLFRFDDGRFTRFGREQGLSIEIVNT
                     IHEAEDGTLWVGTYGGGLNRFRDGLFSAVTTREGLYDDAVFAILPDGQGNLWISCNRG
                     IYAVSLRELDAVARGAARRVRCRPFGVEDGMRNRECNGANQPAGARGPDGRLHFPTIE
                     GIVTVDPAGLFRNPVPPPVHVELVKVDDVVESARSFLELAPGARRFEFQYTAPSLRVP
                     SRVVFRVRLEGVDADWVEVGTRRTAWYTSLSPGSYTFRVAAANESGVWNENEASFAFR
                     LKPRLDQRPFFWLVVGRSPSPSPTRATGFASRSSRSARESSWRWSRSGPGACARRRSG
                     RSGRSRRRNGKGRGPRRQAGPPRRRTARRATSSR"
     gene            5045..6265
                     /locus_tag="P5164_12905"
     CDS             5045..6265
                     /locus_tag="P5164_12905"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY44835.1"
                     /translation="MKKKWILVSALGITLVGAGVVFASRNGKEKPAAAAESLPFRLGT
                     VTAEDLQVSVREVGVVDPVTKVDVKSVVSGRVVKLNVREGAVVKAGDVLAEVEPDVTQ
                     AQSLSEVSAAVTQAELGLKDAERQLKAQQALWDAGLVGHETLKDYVARRDVAAESLKA
                     ARTRYRIVEDHGIPISSAASSQVARVASPMTGVVIKRGVELGETVTSGVSSFNAGTVL
                     FTVADLKSLIVKVNLNEVDIAKVGVGQPVRITLDAYPQKVFGGKVRFVAPAATLVEKV
                     KVFEIEVALDELGDAFRTGMSANVEILGEKRTKALSLPLEALQKREGETVAFVLKKDL
                     DPKAVAKAKEGLSGRAKFVWLSENWKDYFNPVPVKAGIATLERVEVLAGLTPGDKVCL
                     EDPTRKKVEKDEDN"
BASE COUNT          889 a         2268 c         2206 g          920 t
ORIGIN      
        1 tcggcccact cgacggtgta gaggtcggcc acgtcccgcg agtcgagctt ctcgaccgtc
       61 tcgcgggcga ggtccgccca ggcgcggcgc gttcccatct cgctccttgt acgtcccgcc
      121 gggagggaag ttcaaccccg ccgggcggct cccgaggtcc cgcccggcgg ggcggcgaaa
      181 aaaagaggcc cgcgacgcct gtgccgcggg ccgtccggga taggggtcga cgggatcagc
      241 ggaggatctt ccggacttcc tcctcgagga ggcccgggac gaggggctcg agctcgtagc
      301 tgacgcgggt cgcgggcttg ccgatcttca ggacccgggt caggtcgatc ggggttcccg
      361 agacgacggc gtcgcacggc gtcttctcga tcgtggcctc gagctcggcg atctgcagcg
      421 ggctgtaccc catcgccggg aggatcccct cggcgttcgg gtacttctcg taggtcgcct
      481 tgatcgagcc gacggcgtac ggcgtcgggt cgacgatctc ggccgcgccg agcttcttcg
      541 ccgcgacgac gcccgccccg taggtcattc ctccgtgcgt gagcgtcggg ccgtcctcga
      601 tgacgaggac gcgcttgccc ttcacgagct ccggcttctc gcaggtggcg ggcgagttcg
      661 cgcggaggac gcgcgccttc gggttcacct tcgccgcgac ggcctcgatc gcctcgatcg
      721 ccttcgcctc ggccgagtcg cacttgttga tgacgatgac gtcggcgcgg cggaagttcg
      781 cctcgcccgg gtggaagagc atctcgtggc ccggccggag cgggtcggcg atgcagatgt
      841 ggaggtccgg gcggtagaac ggcaggtcgt tgttcccgcc gtcccagagg atgacctcgg
      901 cctccgactc ggccgccttc aggatctgcc cgtagtcgac gcccgagtag atgatgaagc
      961 cgttgtcgat gtgcggctcg tactcctcgc gctcctcgat cgtgcacttg tggtggtcga
     1021 ggtccgcgta gctcgcgaag cgctggcagg cctgcgcggc gaggtcgccg tacggcatcg
     1081 ggtggcggat cgcgacgacc ttcttcccga gcttcttgag gatcgacgag acgtagcggg
     1141 tcgtctggct cttccccgcg ccggtccgca ccgccgtgat cgcgacgacg ggagccatcg
     1201 acgggatcat cgtccggtcg ttcgagagga gctcgaagtc ggcccccgcg acgatgcagc
     1261 gcgaggcgag gtgcatgacc gtctcgtggg agacgtccga gtaggagaag acgcaggcgt
     1321 cgatccgctc gtcgcggatc aggcgctcga ggtcgtcctc cgggacgatc cggatcccct
     1381 gagggtagcg ggagcccgcg agctcgacgg ggtagcggcg tccggcaatg cccggaatct
     1441 gcgtggcggt gaacgcgacg acctcgaccg acgggtcgtc gcggtaggtc gtgttgaagt
     1501 tgtggaagtc ccggccggca gcgccgatga tcgcgagccg acgtacgcct ttcgccatag
     1561 agggagtctc cttgggccgc attctaggcc gctccggagg ctcccggaac ccccttgggg
     1621 tcggagctac attttacata cgatcctgag atccgctctc ccgttcgggg ttcgggccgg
     1681 cttccgcggg tcgctccggg agctcgacga cgaacgtcga gccctccccg ggccggctct
     1741 cgacgaagac gtcgcccccc atcatccggg cgaggcggcg tgtgatgacg aggccgagcc
     1801 cggtccctcc gaagcggcgg gcgacctggg ggtcggcctg ctcgaacgcc cggaagaggc
     1861 gtcccacctg ctcctccgtc atgccgatcc cggtgtcggt cacggagaag cggacccggc
     1921 cgccttcggg tccggcgagg cgctgcacgc ggaaggcgac ctcgccgtcg tgggtgaact
     1981 tgcccgcgtt cgcgaggagg ttcaggagga cctgccggag gcgcgtcggg tcggtgacga
     2041 gccgccccgc cccgtccgcc ccctccacga cgaagcggtt ccggttccgc tcgacgaggg
     2101 gcctcatcgt cgtgacgacg ccggcgagga gcggcgcgag ctccgtctcc tcgacggaga
     2161 gctcgagctt ccccgcctcg agcctcgaaa ggtcgaggag gtcgttgatc aggccgagga
     2221 ggtgccggcc cgcggagtgg accttctcga cgtcctcggc gagcccgggc aggcggcgct
     2281 cggagatctc ctccatcagc agctcgctgt agccgaggat cgcgttcagc ggcgtccgga
     2341 gctcgtggct cgtcatcgcg aggaagtcgc tcttcgtgcg gttcgcctcc tcggcggccc
     2401 ggcctgcctc ctcggccctc tccctttgcc gttccgcctc cgcgagcgcc cgctccgtcc
     2461 gctccttctc ctcgcgcagg ctccgggtcc gctcctcgac cacctccacg agctctctct
     2521 cgcggacctc gagctgcgcg acgcgaaacc ggtagcccgt gtaggcgacg gcgagggcga
     2581 ccgtccgacg acgagccaga agaacgggcg ctggtcgagg cggggcttca ggcggaacgc
     2641 gaacgaggct tcgttctcgt tccagacgcc gctctcgttc gccgcggcga cccggaacgt
     2701 gtacgagccc ggcgagaggc tcgtgtacca ggccgtccga cgcgtgccga cctcgaccca
     2761 gtcggcgtcg accccctcca gccggacccg gaagacgacc cgcgacggga cgcggaggct
     2821 cggcgcggtg tactggaact cgaaccggcg cgcccccggc gcgagctcga ggaagctccg
     2881 cgccgactcg acgacgtcgt ccaccttgac gagctcgacg tggaccggcg gcggcaccgg
     2941 gttccggaag agccctgccg gatcgacggt gacgatcccc tcgatcgtcg ggaaatgaag
     3001 acgcccgtcg gggccccggg cgccggcggg ctggttcgcc ccgttgcact cccggttgcg
     3061 catgccgtcc tcgacgccga acggccggca ccgcacccgc cgcgccgcgc cgcgggccac
     3121 cgcgtcgagc tcgcggagcg agaccgcgta gatcccgcgg ttgcacgaga tccagaggtt
     3181 cccctggccg tccgggagga tcgcgaagac ggcgtcgtcg tagagcccct cgcgcgtcgt
     3241 cacggccgag aagagcccgt cgcggaagcg gttcagccct ccgccgtagg tcccgaccca
     3301 gagcgtcccg tcctccgcct cgtggatcgt gttgacgatc tcgatcgaaa gcccctgctc
     3361 ccgcccgaag cgcgtgaacc gtccgtcgtc gaagcggaag agtcctcccc cgttcgtccc
     3421 gatccagagg ttcccgtcgc ggtccgggag gagcgcgtgg accgtctcgt tccggaggcc
     3481 ctcgccggct ccccatctgc gataggtccc gtcgggcccg atccgggtca gcccgccgcc
     3541 gcgcccgccg aggaagacgc tcccgtcccg ggtgccggtg atcgcgcaga tcgagtcgct
     3601 cgcgagcccg tccgccctgc ggagggtccg gacgacccgc ccctgccgca cgatgctgac
     3661 cccgcccccg cgggtgccga gccagacggc cccgtcgggc gcctgccaga tcgcctggac
     3721 gcccggagcg gcgagacctt cggccgtgcc gatcgtcgtc acccggtccc cgcgccggac
     3781 gttgacgccg gccgagtcgg tccccgacca gacggccccg tcccggtcga cgaagacggc
     3841 ccaggcgatg tccgcggaga ggccttccgc ggccccccag ggagtgaagg aggcatccga
     3901 gagcttgttc aggccgccgt cctgggtccc gacccagagc gtcccctcgc ggtcttccag
     3961 gagggcgagg acgttcggcg cggggagccc ctcgcggagc gtgaacgccg agagccggcc
     4021 gccggcccac cgggcgagcc ccccggacgt cccgatccag agcgtgccgc gccggtccct
     4081 gagcgcgcag ttgacgacct cgcccgggag cccgtccgcc cggccgagag cctcgacacg
     4141 cccctcccgg aggagacgca gcccgtgggc ggtcccgacg acgagcgtcc cgtcggcgcc
     4201 cgcgtggatc gactggaccg cgtcggtgac tcccgtcgtg acgaaacgcc ggcccgagcg
     4261 gcggaagagc ccgcggtccg tccccgccca gacgacgccc gcctcgtcca ccgcgaccgc
     4321 ccgcaccgcc cctccgggaa ggccgctcga cgcctcacgg gtttcgaagg agcctccggc
     4381 ccgggccgcg agacccgtct cggtcccgat ccagagcgtt ccggcaccgt cctcggcgag
     4441 gctcctcacg atgtcgctcc cgagcccctc cgagcgcccg aacgtccgga tcgtcccgtc
     4501 ctggaggacg ctcagcccgc ctccctccgt cccgagccag agccgcccgc tccggtcctc
     4561 gaggagagcc acgacgcggt tgtggcggag ctccggcgcc gaagccttgt cccacacgac
     4621 gaagcggacc ccgtcgaacc gggccagccc ctcctgcgta ccgatccaga ggtagccgtc
     4681 gcgcgtctgg gccagcgcct cggccgagct ctggggcaga ccttcgcgca cgccccacac
     4741 ctcgtgccgg tactgggaga tcgctttgcc ggggtcgagc gcctccgcag gggcagcggt
     4801 gaggaggatc gccaggatcc ccggggcgag cgcgcgtggg ccgggcgcca tggcgtggac
     4861 cataccgtag aagcggggcc gagccggaga gcccgcgggc tcgcctgtga cgtttcgtct
     4921 cgttcgggcg tcccgacgaa ccttctcacc gggcagccgt atcgcctttt cgaccttcga
     4981 cggaatctcc cgcccgaagg gacacgaacc cgttcacggg atcgacgatc tcggagtgcg
     5041 cgccatgaag aagaagtgga tcctcgtctc ggccctcgga atcacgctcg tcggtgccgg
     5101 cgtcgtcttc gcgtcgcgca acggcaagga gaagcccgcc gccgcggccg agagcctgcc
     5161 gttccggctc gggaccgtca ccgcggagga cctccaggtg agcgtccggg aggtcggggt
     5221 cgtcgacccg gtcacgaagg tcgacgtgaa gtcggtcgtc tcggggcgcg tcgtcaagct
     5281 gaacgtccgc gagggagcgg tcgtgaaggc gggggacgtc ctcgccgagg tggagcccga
     5341 cgtcacccag gcccagtcgc tctcggaggt ctcggccgcg gtgacgcagg ccgagctggg
     5401 gctgaaggac gcggagcggc agctgaaggc ccagcaggcg ctctgggacg ccggcctcgt
     5461 cgggcacgag acgctgaagg actacgtcgc ccgtcgcgac gtcgccgccg aatcgctcaa
     5521 ggcggcgcgg acccgctacc ggatcgtcga ggaccacggc atcccgatct cctccgccgc
     5581 ctcctcgcag gtcgcgcggg tcgcgagccc gatgacgggc gtcgtcatca agcggggcgt
     5641 cgagctcggc gagacggtca cctccggcgt ctcctccttc aacgccggca ccgtcctctt
     5701 caccgtcgcc gacctgaagt cgctcatcgt gaaggtgaac ctgaacgagg tcgacatcgc
     5761 gaaggtcggc gtcggccagc ccgtgaggat cacgctcgac gcctacccgc agaaggtctt
     5821 cggcgggaag gtccgcttcg tcgccccggc ggcaacgctc gtcgagaagg tgaaggtctt
     5881 cgagatcgag gtcgccctcg acgagctcgg cgacgctttt cggaccggga tgagcgcgaa
     5941 cgtcgagatc ctcggcgaga agcggacgaa ggcgctctcg ctcccgctcg aggcgctcca
     6001 gaagcgcgag ggggagacgg tcgccttcgt cctgaagaag gacctcgacc cgaaagccgt
     6061 cgcgaaggcg aaggaaggcc tctcgggccg ggcgaagttc gtctggctct ccgagaactg
     6121 gaaggactac ttcaaccccg tcccggtgaa ggccggcatc gcgaccctcg agcgcgtcga
     6181 ggtcctcgcg ggcctcaccc ccggcgacaa ggtgtgcctc gaggacccga cccgcaagaa
     6241 ggtcgagaag gacgaggaca actgagcatg gacgccgtca tcg
//
LOCUS       DAOTEZ010000247         6264 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_8941_length_6264_cov_5.227412, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000247 DAOTEZ010000000
VERSION     DAOTEZ010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6264
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8941_length_6264_cov_5.227412"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="P5164_12910"
     CDS             <1..803
                     /locus_tag="P5164_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY44836.1"
                     /translation="AAVLLVAGVPPALATLGGAAASAVAGTVTGLLATRFRIPSLLAG
                     ILVMTALYSVNLRVMGRSNVPLLSVPSLAETAERAGRKLAGGAESVGVLGWEVAVRDL
                     AMLAGAFLVSFAVAALLYLFFRTNLGTAMRATGNNAQMIRALGVDTGRMTIVGLALAN
                     GLVGLSGALLAQYQGFADVQMGIGMVVWGFASVIIGEALVRSRLLGLALAGTVLGCVL
                     FRLLIALALRWGLDPNDLKLVTAAFVFAALVLPRLLERASRRKEASRA"
     gene            796..1608
                     /locus_tag="P5164_12915"
     CDS             796..1608
                     /locus_tag="P5164_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY44837.1"
                     /translation="MLEVRGLEKTFHARTADEVRALRGVDLSVADAAFVVVIGTNGSG
                     KSTFLNAVAGTFLPDAGTVRLDGEDVTRWPEERRARKVGRVFQNPFSGTAPAMTIAEN
                     LALAARRGKVRGLGPALPAALRREVRDRVAALGMGLEDRLASEIGTLSGGQRQALTLL
                     MATWVRPRLLLLDEPTAALDPKTAEQVIRLTKEVVEKDRLTTMMVTHSMAQAVHLGDR
                     LVMMHRGRVIHEAAGEEKRRLRPEDLLARFEDVRRADRLDAAAADLLRRLYV"
     gene            1755..2540
                     /locus_tag="P5164_12920"
     CDS             1755..2540
                     /locus_tag="P5164_12920"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRY44838.1"
                     /translation="MRRIASSLLLGALVVTGPSKAQPPSKLLDMTYPFEDASVYWPTA
                     TPFRLTRVSFGPTPGGWWYASNDFTASEHGGTHADAPIHFAEGGRTMERIPLEEWIGP
                     AVKVDVSAACAKDRDYMLTVADLKAWEERHGRIPKGAWVVMYTGHGTRAYPDPVAVLG
                     TDRRGADAIPLLSFPGFSPEAADFLVKERSIRGIALDTPSIDRGRSPDFRVHQIICGA
                     DRLALENLAAADRLPAKGATLYVVPIPIRGGSGAPARVFAVLP"
     gene            2601..2933
                     /locus_tag="P5164_12925"
     CDS             2601..2933
                     /locus_tag="P5164_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRY44839.1"
                     /translation="MDLTTREESGCTVARVAGRLDAATAPLFEKACGEWVDKGTKKLV
                     LDLSALDYVSSAGLRAVLVTMKRLKSAGGGIAVSGLSGVVKEVFTISGFDALLPTRDS
                     VAEAIASL"
     gene            complement(2982..4073)
                     /locus_tag="P5164_12930"
     CDS             complement(2982..4073)
                     /locus_tag="P5164_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY44840.1"
                     /translation="MIEVEHLSVRFGDQVVLRDVSVRVAKGEVISVIGPSGTGKSTFL
                     RCLNLLQRPTGGSVRVGGVDLLARGADVPRVRRRMGMVFQSFNLFAHLTALDNLTLGP
                     VKLLGTPRPEAEKKAMETLRMVGLAEKAGSLPDELSGGQKQRVAIARCLAMEPEILLF
                     DEPTSALDPTMVSEVLAVMRRLAREGMTMVVVTHEMEFARDVSSRVLYMDEGVVYEEG
                     SPAQVFDAPRRDRTRAFVERRRSATFRVASPDYDLYALNASLEAFCDRHALPRQTRHD
                     VVLLAEELLQIHAPLLPATPLDVVLSYSEKTDRLELAVESKGEAADPLDLARLPDELG
                     LRIVRGLADGITYSSDAGRNRLSMTVRQA"
     gene            complement(4070..4852)
                     /locus_tag="P5164_12935"
     CDS             complement(4070..4852)
                     /locus_tag="P5164_12935"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRY44841.1"
                     /translation="MGGGGTTLAVAWPAVLALAGAAGGPPGTFLRRVAEGFENNLVRE
                     GRYLLILDGLHTTVLISVLAGLLGTALGALVCFLRMSPRRALSVPARAYVALLRGTPV
                     LVLLMLLYYVAFASVNVSAVLVAVLAFGLNLGAYAAEIYRTGIESVDRGQAEAGIALG
                     FTKAAAFRHVVLPQTVRRILPVYKGELVSMVKMTSVVGYIAVQDLTKASDLIRSRTFD
                     AFFPLVLVAVLYFLVSGLLIHALGSVERWTDPRRKAARGGGA"
     gene            complement(4854..5651)
                     /locus_tag="P5164_12940"
     CDS             complement(4854..5651)
                     /locus_tag="P5164_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRY44842.1"
                     /translation="MSRRIAGAVRVAVVLAALGALSGCGTRGEVTSLAQLEGKVFAVP
                     TGTVADTLVLSRFPKAKFQYFNSVLDCAMAVKAGKADAAAYDEPILRNLAAKNEGLTV
                     LPEMITVDQYGFAVRPDDAELKAAIDAVVAGLKADGGYEEMKGRWLPKSGPPAPMPQL
                     PPGGESGVLRLGTAAVTEPFSFVDASRSVVGLDVELAQHVARRLGRRLEVVDMDFGAM
                     IPALLSGKVDVIAACITITEERAKSVLFSEPYYAGGIAALVREAKRP"
     gene            5874..>6264
                     /locus_tag="P5164_12945"
     CDS             5874..>6264
                     /locus_tag="P5164_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRY44843.1"
                     /translation="MKLVTFVRPGADPAGAPGIALPGAGVLDLGRASETFRGLSMLDL
                     VRRHEELLPEARPLAAGEASLPAGTLVPEDEVRLLAPLPRPVSVRDGYAFRQHVETAR
                     RNRGLPMIPEFDEFPVFYFTNALAVIGP"
BASE COUNT          849 a         2354 c         2203 g          858 t
ORIGIN      
        1 cggcggcggt cctcctcgtc gccggcgtcc ccccggcgct cgccacgctc ggcggcgcgg
       61 ccgcctccgc cgtcgccggg acggtcaccg gcctcctcgc gacgcgtttc cggatcccga
      121 gcctcctcgc cggcatcctc gtcatgacgg ccctctactc cgtcaacctg agggtcatgg
      181 gcaggagcaa cgtgcccctc ctctcggtgc cgtcgctggc cgaaacggcc gagcgcgccg
      241 gacgcaagct cgccggcggc gccgagagcg tcggcgtcct cggctgggag gtcgcggtgc
      301 gcgacctggc gatgctggcg ggggcctttc tggtgtcttt cgccgttgcc gcgctcctct
      361 atctcttctt caggacgaac ctcggcaccg cgatgcgggc gacggggaac aacgctcaga
      421 tgatccgcgc cctcggcgtc gacacggggc ggatgacgat cgtcgggctg gccctcgcaa
      481 acggcctcgt cggcctctcc ggcgccctgc tggcccagta ccaggggttc gccgacgtgc
      541 agatggggat cggaatggtc gtctggggct tcgcgagcgt catcatcggc gaggcgctcg
      601 tccgatcccg cctcctcggc ctcgcgctcg ccggcacggt cctcgggtgc gtcctcttcc
      661 gcctcctgat cgcgctcgcc ctccgctggg gcctcgaccc gaacgacctg aagctcgtca
      721 cggccgcgtt cgtcttcgcc gccctcgtcc tgccgcgcct cctcgagcgc gcctcgcggc
      781 ggaaggaggc ctcccgtgct tgaggtccgc ggcctcgaga agaccttcca cgcccggacg
      841 gccgacgagg tccgcgccct ccgcggcgtc gacctctccg tcgccgacgc cgccttcgtc
      901 gtcgtcatcg ggacgaacgg ctcgggcaag tcgacgttcc tgaacgccgt cgccggcacg
      961 ttccttcccg acgccggcac ggtccgcctc gacggggagg acgtgacccg ctggcccgag
     1021 gagaggcggg cgcggaaggt cgggcgcgtc ttccagaatc cgttcagcgg gacggcgccg
     1081 gccatgacga tcgccgagaa cctcgcgctc gccgcgcgcc gcgggaaggt ccgcggcctc
     1141 gggcccgcgc tcccggccgc gctccggcgc gaggtccggg accgggtcgc cgcgctcggg
     1201 atggggctcg aagaccggct cgcgagcgag atcggcacgc tgtccggcgg ccagcgacag
     1261 gcgctcactc tgctgatggc gacctgggtg cgtccgcgcc tgctcctcct cgacgagccg
     1321 acggcggccc tcgacccgaa gacggcggag caggtgatcc ggctgacgaa ggaggtcgtc
     1381 gagaaggacc gcctgacgac gatgatggtc acccactcga tggcccaggc ggtccacctc
     1441 ggcgaccggc tcgtcatgat gcaccgcggc cgtgtgatcc acgaggcggc gggcgaggag
     1501 aagcggcgcc tgcgccccga ggacctcctc gcccgtttcg aggacgtgcg gcgggccgac
     1561 cgcctcgacg cggccgccgc cgacctcctg cgccgcctgt acgtctgaga ccggacgaag
     1621 gagacggaga agagccgatg gagaagcacc cggtcccgac cgcccacccc gccgatcgcc
     1681 tccggcacgt ggccgaggcg cgttccgctc ccccgaggcc cgtcctgcgg ggtgccgccc
     1741 ggggggaggc cccgatgagg aggatcgctt cgtctctcct cctcggcgcg ctcgtcgtca
     1801 cgggcccttc gaaggcccag ccgccgtcga agctcctcga catgacgtac ccgttcgagg
     1861 acgcgagcgt ctactggccc acggcgacgc cgttccggct gacgcgcgtc tccttcggcc
     1921 cgacgccggg cggctggtgg tacgcctcga acgacttcac cgcctccgag cacggcggga
     1981 cgcacgccga cgccccgatc cacttcgcgg agggggggcg gacgatggag cggatccccc
     2041 tcgaggagtg gatcgggccc gccgtgaagg tcgacgtctc ggcggcctgc gcgaaggacc
     2101 gcgactacat gctgaccgtc gccgacctga aggcctggga ggaacggcac ggccggatcc
     2161 cgaagggagc ctgggtcgtc atgtacacgg gccacggcac gcgggcctac cccgaccccg
     2221 tcgcggtcct cgggaccgac cggcgcggag ccgacgcgat ccccctcctg agcttccccg
     2281 gcttctcgcc cgaggccgcg gacttcctgg tgaaagagcg ctcgatccgg gggatcgcgc
     2341 tcgacacgcc gagcatcgac cgcggcaggt cgcccgactt ccgggtccac cagatcatct
     2401 gcggcgccga caggctcgcc ctcgagaacc tcgccgcggc cgaccgcctg ccggcgaagg
     2461 gggcgacgct ctacgtcgtc ccgatcccga tccggggggg gagcggcgcc cccgcgcgcg
     2521 tcttcgcggt cctgccctag gccggaagga ccgcgcccgc cgggcgggcc ggcgggctaa
     2581 catccggacg gaggagcccg atggacctga cgacgcgtga ggaatccgga tgcacggtgg
     2641 cgagggtcgc cggacggctc gacgccgcga ccgcccccct gttcgagaag gcgtgcgggg
     2701 agtgggtcga caaggggacg aagaagctcg tcctcgacct ttcggcgctc gactacgtga
     2761 gcagcgcggg tctccgggcc gtcctcgtca cgatgaagag gctcaagtcc gccggcggcg
     2821 ggatcgccgt gagcgggctc tccggcgtcg tcaaggaggt cttcacgatc tccggattcg
     2881 acgcgctcct cccgacccgg gactcggtcg ccgaggcgat cgcgagcctc tgacgtcccc
     2941 cggggccggc gtgccccgcc gccgcccccc ggcgggttcc ctcacgcctg ccggaccgtc
     3001 atcgacagcc ggttgcggcc ggcgtcgctc gagtacgtga tcccgtccgc gagcccccgg
     3061 acgattcgca ggcccagctc gtccggcagc cgcgccaggt cgagcgggtc cgcggcctcc
     3121 cccttcgact cgaccgccag ctcgagccgg tcggtctttt ccgagtacga gaggacgacg
     3181 tccagcggcg tcgccggcag gagcggggcg tggatctgga gcagctcctc cgcgaggagg
     3241 acgacgtcgt gccgcgtctg ccgcggaagg gcgtgccggt cgcagaaggc ctcgagagag
     3301 gcgttcagcg cgtagaggtc gtagtcgggg ctggcgacgc ggaacgtcgc gctccggcgc
     3361 cgctcgacga aggccctcgt ccggtccctc cggggcgcgt cgaagacctg cgccgggctc
     3421 ccctcctcgt agacgacccc ctcgtccatg tagaggacgc ggctcgagac gtcccgggcg
     3481 aactccatct cgtgggtgac gacgaccatc gtcatcccct cgcgcgccag ccgccgcatc
     3541 acggcgagga cctcgctcac catcgtcggg tcgagcgccg aggtcggctc gtcgaagagc
     3601 aggatctcgg gctccatcgc gagacagcgc gcgatcgcca cgcgctgctt ctgccctccg
     3661 gagagctcgt cggggagcga gccggccttc tcggcgagcc ccaccatccg gagcgtctcc
     3721 atcgccttct tctcggcctc cggccgcgga gtcccgagga gcttcacggg gccgagggtc
     3781 aggttgtcga gcgccgtcag gtgcgcgaag aggttgaacg actggaagac catccccatc
     3841 cgccggcgga cccgcgggac gtcggcgccg cgcgcgagga ggtcgacgcc gccgacgcgc
     3901 accgatcccc cggtcggcct ctggaggagg ttcaggcagc gcaggaaggt gctcttgccc
     3961 gtcccggagg ggccgatgac cgagatcacc tcgcccttcg cgacccggac gctcacgtcc
     4021 cggaggacga cctggtcgcc gaagcggacc gacagatgct cgacctcgat cacgcgccgc
     4081 ccccgcgcgc ggccttcctc cgcgggtcgg tccatcgctc cacggagccg agcgcgtgga
     4141 tcaggaggcc ggagacgagg aagtagagga cggccaccag gacaaggggg aagaaggcgt
     4201 cgaaggtccg gctgcggatg aggtcgctcg ccttcgtcag gtcctggacg gcgatgtagc
     4261 cgacgaccga cgtcatcttc accatcgaga cgagctcgcc cttgtagacc gggaggatcc
     4321 gcctcaccgt ctgggggagg acgacgtggc ggaaggcggc ggccttcgtg aacccgaggg
     4381 cgatccccgc ctccgcctgc ccgcggtcga cgctctcgat gcccgtccgg tagatctcgg
     4441 ccgcgtaggc gccgaggttg agcccgaagg ccaggaccgc gacgaggacc gcgctgacgt
     4501 tcaccgaggc gaacgccacg tagtagagga gcatcaggag gacgaggacc ggcgtcccac
     4561 ggaggagcgc gacgtaggcg cgcgcgggga ccgaaagggc ccgtcgcggc gacatgcgga
     4621 ggaagcagac cagcgccccg agcgcggtcc cgaggaggcc ggctaggacc gagatgagca
     4681 ccgtcgtgtg gaggccgtcg aggatgagga ggtagcggcc ctcccggacg aggttgttct
     4741 cgaagccctc ggcgacgcga cgcaggaacg tgccgggggg gcctcccgcc gccccggcga
     4801 gcgcgaggac cgccggccac gcgacggcga gcgtcgtccc gcctcctccc acgtcaggga
     4861 cgcttcgctt cccggacgag ggcggcgatc ccgccggcgt agtacggctc ggagaacagg
     4921 acgctcttcg cccgctcctc ggtgatcgtg atgcaggcgg cgatcacgtc gaccttgccg
     4981 gagagaaggg cggggatcat cgccccgaag tccatgtcga cgacctcgag ccggcgtccg
     5041 agcctccgcg cgacgtgctg cgccagctcg acgtcgagcc cgacgacgct ccgcgaggcg
     5101 tcgacgaagg agaacggctc cgtgaccgcg gccgtgccga gcctcaggac gcccgactcg
     5161 cccccgggcg gaagctgcgg catcggcgcg ggcggccccg acttcggcag ccagcgtccc
     5221 ttcatctcct cgtacccgcc gtcggccttc agcccggcga cgaccgcgtc gatcgccgcc
     5281 ttcagctccg cgtcgtccgg gcggaccgcg aagccgtact ggtcgaccgt gatcatctcg
     5341 gggaggaccg tgagcccctc gttcttcgcg gcgaggttcc ggaggatcgg ctcgtcgtac
     5401 gccgcggcgt cggccttgcc ggccttcacc gccatcgcac agtcgaggac gctgttgaaa
     5461 tactggaact tcgccttcgg gaacctcgag aggacgagcg tgtcggccac cgtccccgtc
     5521 gggacggcga agaccttccc ctcgagctgt gcgaggctcg tcacctcgcc ccgcgttccg
     5581 caccctgaca gcgcgccgag agcggcgagg acgacggcga cacggacggc tccagcgatt
     5641 ctcctggaca tctcgactcc tccttcgatg ctcgggccgc agcggcggcg gccggtcgcg
     5701 tccggacccg agcggtcccc gcggcggacg gctcccgggc tcctgcgccc ctccggcgct
     5761 tcccgggcgg ctccgcgaag cctcgcgccg aggttaccaa gaggaccgcc cggacggcga
     5821 gcccgcccac ggcgcgcccc cccgctccgg gcgttgtcgt ataacggcgg gcgatgaagc
     5881 tcgtcacgtt cgtccgcccc ggggcggacc cggccggcgc tcccgggatc gccctccccg
     5941 gcgccggcgt gctcgacctc ggccgcgcct ccgagacgtt tcgcggcctc tcgatgctcg
     6001 atctggtccg gcgccacgag gagctcctcc ccgaagcccg cccgctcgcg gccggagagg
     6061 cttcccttcc cgccgggacc ctcgtccccg aggacgaggt ccgcctcctc gcgccgctcc
     6121 cgcgccccgt ctccgtccgc gacggctacg cgtttcgcca gcacgtcgag acggcccggc
     6181 gcaaccgcgg cctgccgatg atcccggaat tcgacgagtt ccccgtcttc tacttcacga
     6241 acgccctcgc ggtgatcggc ccgg
//
LOCUS       DAOTEZ010000248         6258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_8950_length_6258_cov_5.520393, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000248 DAOTEZ010000000
VERSION     DAOTEZ010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8950_length_6258_cov_5.520393"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="P5164_12950"
     CDS             complement(<1..340)
                     /locus_tag="P5164_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY44844.1"
                     /translation="MRSKAKGNVLVAGAGSALARAVAEGLAARGCGLVLAARDADEAA
                     RTAADLALRFEVATAALGFDARDAAAAAALPARAEEALPGGLAGVLVAFAVMPDQELV
                     ERDTEALLELA"
     gene            complement(337..1746)
                     /locus_tag="P5164_12955"
     CDS             complement(337..1746)
                     /locus_tag="P5164_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRY44845.1"
                     /translation="MSGSPDASASRAEGFPYRAAHLSLSGWGRFAVEAGPAYRPETWR
                     AVEAAVRSAPEPTLALRGLGRSYGDSALNGAGAVVLTERLDRILAFDPEGRTLTCEAG
                     TSLARLLAFLLPRGFFLPVVPGTRFVTVGGALAADVHGKNHHVDGTIGRHVEELTLLL
                     SSGETLRCSPRENAEAFDATIGGMGLTGVILSARLALLPVETPWVRVVKRRGRCLEEV
                     LARLEEEESLSRYSVAWIDGLARGRALGRGVLMRGDHVLAAELPRGKAVRGSLPEKPG
                     PNVPFEAPSALLSPLTISAFDALYYAAGRDSTGLSGPASFFFPLDALGSWNRLYGRRG
                     FVQYQALLPRETAAEGCRRILEEISRERAASFLAVLKTTGPEGRGVLSFPKPGVTLGL
                     DVPNVGERLRPLARRLDEILLALGGRLYLAKDALTDAPTFAAMYARLPEFLAVKRRLD
                     PNGRFSSSQARRLGLAEAR"
     gene            complement(1776..3113)
                     /locus_tag="P5164_12960"
     CDS             complement(1776..3113)
                     /locus_tag="P5164_12960"
                     /inference="COORDINATES: protein motif:HMM:NF005560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRY44846.1"
                     /translation="MTDAPSPAPARRKSVAVVGGGLAGLVAADRLLGAGLPVTVFEKY
                     PEAGGLVGTFSVGGEPLERFYHHLFTSDADYVSLAEELGLGGEIEWLPSKMGFYSNGL
                     LYPFGTPLSLLGFAPLGLRGRVELVLSTLKLRGMSDWRALEGETAVGWLRKNGYGRVL
                     DVVWGPLLEQKYGRRATEVGLVWLWGKIALRTRSRDKSGLGERLGYMRGSFGRVVDAL
                     LARIAERGGEIRPARPVKLATRAGQGWSVESRGGAETFDIVLSTVAIPELLRLVPDLP
                     SDLRALWGTISYAHALCPVLVLDRPLTPYYWTNVGDATMPFGGFIEHTNYVPAERYGG
                     RHVVYLSDYVLPDDPKWTMPDRDLWELYLPAVSRLSPAFEKAKVLERHLFRAEYAQPI
                     VGTHYARVLPPVRTGLPGLYSAAMAQVYPEDRGQNYAVKIAREAAAALLDDLG"
     gene            3199..5238
                     /locus_tag="P5164_12965"
     CDS             3199..5238
                     /locus_tag="P5164_12965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HRY44847.1"
                     /translation="MTVASALLLAVLLAGLLAAGTAVVLRAGRGDGDADGVRDGALAL
                     GLPVGLLLLAFPGQVASAVGGVPFGVVLALGGVAVVAALALCGKELAVLRERRAFAAV
                     ALLLALFAFFLWLRLGWGDARQTEKPMDLAVLSALLVTPSLPLEDPWLAGTAFPYYHF
                     GTYLLALPFRLAGIAPGVAYNLVAALVAALAGGAAFGVVRLRGGGRRLAVAAALLLTL
                     AGTWDGARPLLGGGSLASVDLWASSRRVAHTITEWPLFTLWLGDLHPHAVALPLLLAL
                     AALAGRFASGAGLLLDAALLAALLSANPWDLPAALLLLGAGAFVSRPFLPALVRALST
                     AAVALPFLVPTLLGARPEMQGLRGVAARTTSPEAFLHFGGLLLVPALAVGVALLRSRD
                     DAEESFLSANLYPAAGVFLAILTGRPVLGLGAAFLLAVLHLLPGLRGALRAGFFLAAA
                     AVALAMLPELVAVKDPYGEELHRMNTVFKCYAGAAALLAPAAALLAPLALATRRARYA
                     VRAALALGALALLAHPAALLAQRAKATRTSLDGLTWMTREAPGDRAAAEWLRANAPAG
                     ARLVEAVGSAYTDHGRVGGASGRPLVLGWTNHEGLWRGAAGAGAIEARRRDVETIYRS
                     DDVVAVRAALQRTGARYVVVGELERKEHGANALRVRAPLRRVLEAEGTELWEVAP"
     gene            5235..>6258
                     /locus_tag="P5164_12970"
     CDS             5235..>6258
                     /locus_tag="P5164_12970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03663 family protein"
                     /protein_id="HRY44848.1"
                     /translation="MSAASPAYGRRERWAWGMLLLLALLLRVWDLGSRAFHHDESIHG
                     WFTHQLAFQGTYVYDPVYHGPVQYYMVAAAFRLLGDSDFSARLPAALGGVGLVAMAML
                     LRARFGRRAAFASGVLLAISPNLLYYTRFCREDVWSLLGTFGLFLWLDRWWATRRVAD
                     LALAAVWGAVAFASKENFYVLGALMAPAVLAAVWEPGRGFPLVPKLRALLDLLERHGA
                     AIAGAILLFFTLSEVAYTLFLVHPESGNPVFQAISYWWGQHKVERVGGPKTYYLPRIL
                     QYEFAIVLPALVWIGLRFRRFSAAERFVSALAVSSVAMYAWLGEKTPWLLVHQVLPFV
                     PLAGAAW"
BASE COUNT          701 a         2282 c         2419 g          856 t
ORIGIN      
        1 cggcgagctc gaggagcgcc tcggtgtcgc gctcgacgag ctcctggtcg ggcatgacgg
       61 cgaaggcgac gaggacgccg gcgaggccgc ccggaagggc ctcctcggcg cgggccggga
      121 gcgcggcggc cgccgcggcg tcgcgcgcgt cgaagccgag ggcggccgtg gcgacctcga
      181 atcggagcgc gaggtcggcg gcggtccgcg cggcctcgtc ggcgtcccgc gccgcgagga
      241 cgaggccgca gccgcgggcg gcgagcccct cggcgacggc ccgcgcgagg gccgagccgg
      301 cgccggccac gaggacgttc cccttggcct tgcttctcat cgcgcctccg cgaggccgag
      361 gcggcgcgcc tgcgaggagg agaagcggcc gttcgggtcg agccgccgct tcaccgcgag
      421 gaactcgggg aggcgcgcgt acatcgcggc gaacgtcggg gcgtcggtga gcgcgtcctt
      481 cgcgaggtag agccgcccgc cgagcgcgag gaggatctcg tcgaggcgcc gggcgagcgg
      541 gcggagccgc tcgccgacgt tcgggacgtc gaggccgagc gtgacgccgg gcttcgggaa
      601 ggagaggacg ccgcgcccct ccgggccggt cgtcttcagg acggcgagga aggaggcggc
      661 ccgctcgcgc gagatctcct cgaggatgcg gcggcaccct tcggccgccg tctcgcgcgg
      721 gaggagcgcc tggtactgga cgaagccgcg ccgcccgtag aggcggttcc aggagccgag
      781 cgcgtcgagc gggaagaaga agctcgcggg gccggagagg ccggtggagt cccgccccgc
      841 ggcgtagtag agggcgtcga aggcggagat cgtgagcggg ctgaggaggg ccgagggcgc
      901 ttcgaacggg acgttcggcc cgggcttctc ggggagggag ccgcgcacgg ccttcccgcg
      961 cgggagctcg gcggcgagga cgtggtcgcc gcgcatcagg acgccgcgcc cgagcgcccg
     1021 gccgcgggcg aggccgtcga tccaggcgac ggagtagcgg ctgaggctct cctcctcctc
     1081 gaggcgcgcg aggacctcct cgaggcaccg gccgcgccgc ttgacgaccc ggacccacgg
     1141 cgtctcgacc gggaggagcg ccaggcgcgc agagaggatg acgccggtca gccccatgcc
     1201 gccgatcgtg gcgtcgaagg cctcggcgtt ctccctcggg gagcagcgga gcgtttcgcc
     1261 cgaggagagg aggagggtca gctcctcgac gtgccgcccg atcgtcccgt cgacgtggtg
     1321 gttcttgccg tggacgtcgg ccgcgagcgc gccgccgacg gtgacgaagc gggtgcccgg
     1381 gacgaccggg aggaagaagc cgcgcgggag gaggaacgcg aggaggcgcg cgagcgaggt
     1441 gccggcctcg cacgtgaggg tccgcccctc ggggtcgaac gcgaggatcc gatcgagccg
     1501 ctcggtcagg acgacggcgc cggccccgtt cagcgccgag tcgccgtagc tccttccgag
     1561 gccgcgcagg gcgagcgtcg gctccggcgc ggagcggacg gccgcctcta cggcgcgcca
     1621 cgtctcgggg cggtaggcgg gccccgcctc gacggcgaag cgtccccagc ccgagaggga
     1681 gaggtgggcc gcgcggtagg gaaagccctc ggcgcgcgaa gcggaggcgt cgggagagcc
     1741 gctcaacccc gccatgatag atgcgcgccg cggcgtcagc cgaggtcgtc gaggagcgcc
     1801 gccgcggcct cgcgggcgat cttcacggcg tagttctggc cgcggtcctc ggggtagacc
     1861 tgcgccatcg ccgcggagta gaggcccggg aggccggtcc tcaccggcgg gaggaccctc
     1921 gcgtagtgcg tcccgacgat cggctgcgcg tactcggccc ggaagaggtg ccgctcgagg
     1981 accttcgcct tctcgaacgc gggggagagg cgcgagacgg cggggaggta gagctcccag
     2041 aggtcgcggt cgggcatcgt ccacttcggg tcgtcgggga ggacgtagtc ggagaggtag
     2101 acgacgtggc gccctccgta gcgctcggcc gggacgtagt tcgtgtgctc gatgaagccg
     2161 ccgaacggca tcgtcgcgtc gccgacgttc gtccagtaat agggagtgag cggccggtcg
     2221 aggacgagga cggggcagag ggcgtgcgcg taggagatcg tcccccagag cgcgcggaga
     2281 tctgagggga gatcggggac gaggcggagg agctcgggga tggcgacggt ggagaggacg
     2341 atgtcgaacg tctccgcccc tccacgggac tcgacactcc atccctggcc ggcgcgggtc
     2401 gcgagcttca cggggcgggc ggggcggatc tccccgccgc gctcggcgat gcgcgcgagg
     2461 agcgcgtcga cgacgcgtcc gaacgaccct ctcatgtagc cgagccgctc gccgagtccg
     2521 ctcttgtcgc gcgagcgggt gcgcagcgcg atcttccccc agagccagac gaggccgacc
     2581 tcggtcgccc gccggccgta cttctgctcg aggaggggcc cccagacgac gtcgaggacg
     2641 cgcccgtagc cgttcttccg gagccatccg acggcggtct ccccctcgag cgcccgccag
     2701 tcgctcatgc cgcgcagctt cagcgtggag aggacgagct cgacgcggcc ccggaggccg
     2761 agaggcgcga acccgaggag ggagagcggg gtgccgaacg ggtagaggag gccgttcgag
     2821 tagaagccca tcttcgacgg gagccactcg atctcgccgc cgaggccgag ctcctccgcg
     2881 agggagacgt agtcggcgtc gctcgtgaag aggtggtggt agaagcgctc gagcggctcg
     2941 ccgccgacgg agaaggtgcc gacgaggccg cccgcttccg ggtacttctc gaagacggtg
     3001 acggggaggc ccgcgccgag gagccggtcg gccgcgacga ggccggcgag gccgccgccc
     3061 acgacggcga cgctcttcct tcgggcgggg gcgggagacg gcgcgtcggt catcggccga
     3121 ttgtggacga gaacggagcg gggggaaccc gagcggccgc gaaggcccgc gggcgctgtg
     3181 ctaccgtcgc ccggctccgt gaccgtcgct tccgcgctcc tgctggccgt cctcctcgcc
     3241 ggcctcctgg cggccggaac ggccgtcgtc cttcgcgccg gtcgcggcga tggagacgcg
     3301 gacggcgtcc gcgacggagc gctcgcgctc gggcttccgg tcgggctcct cctcctggcg
     3361 ttccccggcc aggtcgcgag cgcggtcggc ggggtcccct tcggcgtcgt cctcgcgctc
     3421 ggcggcgtcg ccgtcgtggc cgccctcgcc ctctgcggga aagagctcgc ggtcctccgc
     3481 gagcggcggg cgttcgccgc cgtcgcgctc ctcctcgccc tcttcgcctt cttcctctgg
     3541 ctccgtctcg ggtgggggga cgcgcggcag acggagaagc cgatggacct cgcggtcctc
     3601 tcggcgctcc tcgtcacgcc gagcctgccg ctcgaggacc cgtggctcgc cgggaccgcg
     3661 ttcccctact accacttcgg gacgtacctc ctcgcgctgc cgttccgcct cgccgggatc
     3721 gcgccgggcg tcgcctacaa cctcgtcgcc gcgctcgtcg cggcgctcgc ggggggagcg
     3781 gcgttcgggg tcgtgaggct gcgcggcggc ggccggcggc tcgccgtcgc cgcggcgctc
     3841 ctcctgacgc tcgccgggac gtgggacggc gcgcgtccgc tcctcggcgg cgggtcgctc
     3901 gcgtcggtcg acctctgggc ctcctcgagg cgcgtggccc acacgatcac ggagtggccc
     3961 ctcttcacgc tctggctcgg cgacctccac ccgcacgccg tggcgctgcc gctcctcctc
     4021 gcgctcgcgg cgctcgccgg gcgcttcgcg agcggcgcgg ggctcctcct cgacgcggcg
     4081 ctcctggcgg cgctcctttc cgcgaacccg tgggacctcc cggcggcgct cctcctcctc
     4141 ggcgcgggcg cgttcgtctc gcggccgttc ctcccggcgc tcgtccgggc cctctcgacg
     4201 gccgccgtcg cgctgccgtt cctcgtcccg accctgctcg gcgcccggcc ggagatgcag
     4261 gggctccgcg gggtggcggc ccggacgacg agccccgagg cgttcctcca cttcggcggg
     4321 ctcctcctcg ttccggcgct cgccgtcggt gtcgcgctcc tgcgctcgcg ggacgacgcg
     4381 gaggaaagct tcctctccgc gaacctctac ccggccgccg gcgtcttcct cgcgatcctc
     4441 accggccggc cggtcctcgg cctcggcgcg gcgttcctcc tcgccgtgct ccacctgctg
     4501 cccggcctcc ggggcgccct tcgggccggg ttcttcctcg cggcggccgc ggtcgcgctc
     4561 gcgatgctcc cggagctcgt cgccgtgaag gacccgtacg gcgaggagct ccaccggatg
     4621 aacacggtct tcaagtgcta cgcgggggcg gcggccctcc tcgccccggc cgcggcgctc
     4681 ctggcgccgc tcgcgctggc cacgcgccgg gcgcggtacg cggtccgcgc ggcgctggcg
     4741 ctcggcgcgc tggcgctcct cgcgcatccg gcggcgctcc tcgcgcagcg cgcgaaggcg
     4801 acgcggacga gcctggacgg cctgacgtgg atgacgcgcg aggccccggg cgaccgcgcc
     4861 gcggcggagt ggctccgcgc gaacgccccc gccggcgcgc gcctcgtcga ggcggtcggc
     4921 tcggcctaca cggaccacgg gcgggtcgga ggagcgtcgg ggcggcccct cgtcctcggc
     4981 tggacgaacc acgagggact ctggcgcggg gcggcggggg ccggggcgat cgaggcccgg
     5041 cggcgggacg tggagacgat ctaccggtcg gacgacgtcg tggcggtgcg cgcggcgctt
     5101 cagcggacgg gcgcgcggta cgtggtcgtg ggggagctgg agcggaagga gcacggcgcg
     5161 aacgcgctcc gcgtccgggc tccactccgc cgcgtcctcg aggcggaagg gacggagctc
     5221 tgggaggtcg cgccgtgagc gccgcctcgc ccgcgtatgg ccggcgcgag cggtgggcct
     5281 gggggatgct cctcctcctc gcgctcctcc tgcgcgtctg ggacctcggc tcgcgcgcgt
     5341 tccaccacga cgagtcgatc cacggctggt tcacccacca gctcgcgttc caggggacct
     5401 acgtctacga cccggtctac cacggcccgg tgcagtacta catggtcgcc gcggcgttcc
     5461 gcctcctcgg cgactcggac ttctccgcgc gcctcccggc ggcgctcggc ggcgtcggcc
     5521 tcgtggcgat ggccatgctc ctgcgcgccc gcttcggccg gcgcgcggcc ttcgcctccg
     5581 gcgtcctcct cgcgatctcg ccgaacctcc tctactacac ccgcttctgc cgcgaggacg
     5641 tctggagcct cctcgggacg ttcggcctct tcctctggct cgaccgctgg tgggcgacgc
     5701 ggcgcgtcgc ggacctcgcg ctcgcggccg tctggggcgc ggtcgcgttc gcgtcgaaag
     5761 agaacttcta cgtcctcggc gctctgatgg cgcccgccgt cctcgcggcg gtctgggagc
     5821 cgggtcgggg cttcccgctc gtcccgaagc tccgcgcgct gctcgacctc ctcgagcgtc
     5881 acggggcggc gatcgcgggg gcgatcctcc tcttcttcac gctctcggag gtcgcctaca
     5941 cgctcttcct cgtccacccc gagtcgggga accccgtctt ccaggcgatc tcgtactggt
     6001 gggggcagca caaggtggag cgcgtcggcg ggccgaagac gtactacctg ccgcggatcc
     6061 tccagtacga gttcgcgatc gtcctgccgg cgctcgtctg gatcgggctc cgcttccgcc
     6121 gcttctcggc ggcggagcgg ttcgtctcgg cgctcgcggt ctcgtcggtc gcgatgtacg
     6181 cgtggctcgg cgagaagacg ccatggctcc tcgtccacca ggtcctcccg ttcgtgccgc
     6241 tcgccggcgc cgcgtggg
//
LOCUS       DAOTEZ010000249         6248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_8968_length_6248_cov_5.048765, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000249 DAOTEZ010000000
VERSION     DAOTEZ010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8968_length_6248_cov_5.048765"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="P5164_12975"
     CDS             complement(<1..318)
                     /locus_tag="P5164_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44849.1"
                     /translation="MELNLEEALEAPVALSHRLEVPVGRLERSELLSLEPVDFSGTLQ
                     KAEPGFVLRGELSVSGQAVCSRCLAPVPFASRGEVSWVFAPAHKRPTAEETELTANDL
                     EVVW"
     gene            441..1529
                     /locus_tag="P5164_12980"
     CDS             441..1529
                     /locus_tag="P5164_12980"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRY44850.1"
                     /translation="MRIAVDARSLAGARTGVGRTLEGLYDALLRLFPGDSSVFLSPRP
                     VTLPALLEGRVEVVPPARPRLPGTLWLQTVAPIEARRAGADLFHGPLGVLPIASPLPG
                     VATVHDLTPVLFPEWHDRKNVAGFAPLLPSTVRKARALVCVSRSTRDDLLLACPEAAG
                     KAHVVPNGLVPPPPATATEPCRPRPYVLSIGTLEPRKNHLRLVAAMESIWDRRPDFPD
                     LVLAGGDGWGMPRFRDRLRRSRHAGRIDAPGWVSPESAAALLRSARVFAYPSLYEGFG
                     LPVLEAMAEGIPVVASSSSSLPEVVGDAGLLPDPADVSAIAASLERACDDDAFRTKAA
                     RAGRERAALFTWEAAARRTRELFEEALS"
     gene            1526..2632
                     /locus_tag="P5164_12985"
     CDS             1526..2632
                     /locus_tag="P5164_12985"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRY44851.1"
                     /translation="MRRLRVALDARKLRDFGIGTYVRGLLGAAAARGEHDLVALVRPG
                     DAPFLPPGVEAVTCEAAEYSFREPAGVRRALARTRFDVFHAPHYVVPLFPPAATVVTI
                     HDLIHLKRPEHATLPKRLYATTMLRRALRSARVVLTVSEAVRRDLAELSPGHAGKVRA
                     VPNGVAPRFLAPFDAAAVERLRRSRGLDAPYVLFLGNDKPHKNLPGLLEAFARLEEHG
                     LPHRLVLAGGAPERREARRAAAAAAGIEDRLVDLGVVPDEEVVPLLTGASALAMPSFL
                     EGFGLPVLEAQAVGTPVVCSDRGGLPEAGGDAALYADLDDPAALAETLRHLLTDATLA
                     ARLSARGRERARLFTWDATFEKVAAAWRDAAEGA"
     gene            2629..3756
                     /locus_tag="P5164_12990"
     CDS             2629..3756
                     /locus_tag="P5164_12990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY44852.1"
                     /translation="MKVALVHDWLTGTRGGEKVLRELARLFPEAPLFTLFHFPGSVPA
                     EVEAREVRTTWLQRVVSPRRDYRSLLPLYFVACEGWDLSGFELVLSTSHCVAKAAKRG
                     PRAFHLCYCHTPVRYLHDQFDDYLRGRGPLARAAARLVRAPLRARDVATVPRVDAFLA
                     NSENVRERIARLYGRTARVVPAPVDDRFYSPAPRERRGLLVVSALAPYKRVDDAIAAS
                     ARLGLPLTVAGFGPEERRLRALAGPNVTFAGSPSDEVLRELYRSAELVLMPGEEDFGI
                     VPLEAQACATPVVALGKGGALETVVDGATGVLYPSPGPEPLARAIDLARSTPFDGARL
                     VANAARFSEAAFRARFLDAARAALLEAGRSDLAEGLPRAEP"
     gene            3775..5175
                     /locus_tag="P5164_12995"
     CDS             3775..5175
                     /locus_tag="P5164_12995"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HRY44853.1"
                     /translation="MIQNQARRLRATFLASDVAATCAALLAAYVVRFETVWPTPLGVQ
                     PFGNYLRLLPVLALLWPAVFYFHRLYPLRRDRSFVDEALAVLIAASLATLLLVGLLSF
                     SRAFSLPRTLLVLFLGLDVLFVSLGRFAIWRYLEKVWSAGVGVRRALVVGAGEAGRAV
                     ADKLLDHPAAGIKPVGFADDDPERRGAEHRGLRVLGTTADVRALLESHQVDTLFLALP
                     VDAYRTMLRILKDVGNEMVDLRFVPDLFQFVTFKAGVEDFDGLPVINLTQRPLEGWNS
                     LVKRSMDVVLSAGGLVALSVLLPFVAFAIWLEDRGPIFYTQERMGLDGRLFRILKFRS
                     MRVGAEDATGAVWALEGDSRRTRVGAFLRSTSLDEVPQLVNILRGDMSLVGPRPERPE
                     FVREFKEKYPQYMLRHRVKAGLTGWAQVHGWRGNTSLSKRIEHDLYYIENWSLALDLR
                     ILWLTATRSFRDENAY"
     gene            complement(5191..5928)
                     /locus_tag="P5164_13000"
     CDS             complement(5191..5928)
                     /locus_tag="P5164_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44854.1"
                     /translation="MRRSVPVVLALLLLVAGSAPAAEPVSKVVEKTLPLAADGRLAID
                     TYKGRVSVVAWDRAEASIRAVVRPDGECDRAAELVERTQVRIEGGGREVRVVSDYEDL
                     PKIWFSFRDDCSSRPFVEYDIRLPQGATLDLSDYKSRISVEGLGGDVSVESYKGVARL
                     SGLSGRLEVETYKGDVRAELARVAGDLRVETYKGEIEVVLPKGSRVALDEEIGRRGRL
                     VAEIEETRSGPRLVAETYKGTIRLREK"
     gene            complement(5991..>6248)
                     /locus_tag="P5164_13005"
     CDS             complement(5991..>6248)
                     /locus_tag="P5164_13005"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HRY44855.1"
                     /translation="RGLPPASASYRRLQRLLRRRGADLTPASAPAETLSTADRLGVGA
                     PSREIVRAYVAESFGGRPTSPAEAERLTALLREARAARSRT"
BASE COUNT          718 a         2495 c         2122 g          913 t
ORIGIN      
        1 ccagacgacc tcgaggtcgt tcgccgtcag ctccgtctcc tcggcggtcg gccgcttgtg
       61 cgcgggcgcg aagacccagg agacttcccc ccggctcgcg aacggcacgg gcgcgaggca
      121 gcgcgagcag accgcctgcc ccgagacgga gagctccccc ctcaggacga atccgggctc
      181 ggctttctgg agagtgcccg agaagtcgac cggctcgagc gaaagcagct cggaccgctc
      241 gaggcgtccc acggggacct cgaggcggtg agaaagagcc acgggcgctt ccagcgcctc
      301 ttccagattc agttccatct cgacccctcc tcgcggaggg tcggcgagtg tagcacgcgc
      361 ctccgagggg ccgccccgcc ccgggcgacg cccctccggg ggcggccgga ccgcaccggc
      421 cgcgcggtac catgcctcga atgcgcatcg ccgtcgacgc ccgctcgctc gccggggcga
      481 ggaccggcgt cggccggacg ctcgaagggc tctacgacgc gctccttcgc ctcttcccgg
      541 gcgactcctc cgtcttcctc tccccccggc cggtgaccct ccccgccctc ctcgaggggc
      601 gcgtcgaggt cgtcccgccc gcccgccccc gtctgccggg gaccctctgg ctccagacgg
      661 tcgccccgat cgaggcgcgg cgagccggag cggacctctt ccacgggcct ctcggcgtcc
      721 tgccgatcgc atcgcccctc ccgggggtcg ccaccgtcca cgacctgacg ccggttctct
      781 tccccgagtg gcacgatcgg aagaacgtcg ccggcttcgc ccctctcctt ccgtcgaccg
      841 tccggaaggc gcgggctctc gtctgcgttt cccgctcgac ccgcgacgac ctcctcctcg
      901 cctgccccga ggcggccgga aaggcccacg tcgtcccgaa cggtctcgtc cctcccccgc
      961 ccgccaccgc gacggagccc tgccgcccgc gcccctacgt cctctcgatc ggcaccctcg
     1021 agccgcggaa gaaccacttg cggctcgtcg cggcgatgga gtcgatctgg gaccggcgcc
     1081 ccgacttccc cgacctcgtc ctcgccggag gcgacgggtg ggggatgcct cgcttccgcg
     1141 accggcttcg ccgctcgcgc cacgccggcc ggatcgacgc tcccgggtgg gtgtcgccgg
     1201 agtccgccgc ggcgctcctc cgctcggcgc gcgtcttcgc ctacccgagc ctctacgagg
     1261 ggttcggcct cccggtcctg gaggcgatgg ccgaagggat tccggtcgtc gcctcgtcgt
     1321 cctcgtcgct cccggaggtc gtcggagacg ccggactcct ccccgatccg gccgacgtct
     1381 ccgcgatcgc ggcctccctc gagcgcgcct gcgacgacga cgccttccgc acgaaggccg
     1441 ctcgcgccgg gcgcgagcgg gccgccctct tcacctggga ggccgccgcg cgacggaccc
     1501 gggagctctt cgaggaggcc ctctcgtgag gcgcctgcgg gtggctctcg acgcgcgcaa
     1561 gctccgcgac ttcgggatcg ggacctatgt ccgcgggctc ctcggcgccg ccgccgcccg
     1621 gggagagcac gacctcgtcg cgctcgtgag gcccggcgac gcccccttcc ttccgcccgg
     1681 cgtcgaggcc gtgacctgcg aggccgcgga gtattcgttc cgcgagccgg ccggcgtccg
     1741 aagggccctc gcgcggacgc gtttcgacgt cttccacgcg ccgcactacg tcgtcccgct
     1801 cttcccgccg gccgcgaccg tcgtgacgat ccacgacctg atccacctga agcgccccga
     1861 gcacgcgacg ctcccgaagc gcctctacgc gacgacgatg ctccggcggg cccttcgctc
     1921 cgcccgggtc gtcctcacgg tctcggaggc ggtccggcgc gacctcgccg agctctcgcc
     1981 cggccacgcg gggaaggtgc gcgccgtccc gaacggcgtc gcgcctcgct tcctcgcccc
     2041 tttcgacgcg gccgccgtgg agcggctgcg ccgctcgcgc gggctcgacg cgccctacgt
     2101 cctcttcctc gggaacgaca agccgcacaa gaacctcccc ggcctcctcg aggcgttcgc
     2161 ccgtctggag gagcacgggc tcccccatcg cctcgtcctc gcgggcgggg ctccggagcg
     2221 ccgcgaggct cgccgggccg ccgccgcggc cgccgggatc gaggaccggc tcgtcgacct
     2281 cggcgtcgtc cccgacgagg aggtcgtccc cctcctcacg ggagcctccg ccctcgcgat
     2341 gccgtcgttc ctcgaggggt tcggcctccc ggtcctcgag gcccaggccg tcggcacccc
     2401 ggtcgtctgc tcggaccgcg gcggcctccc cgaggcgggg ggcgacgcgg cgctctacgc
     2461 cgacctcgac gacccggccg ctctcgccga gacgctccgt cacctcctga ccgacgcgac
     2521 gctcgccgcg cgcctctcgg cgcgggggcg cgagcgcgcc cgcctcttca cctgggacgc
     2581 gacgttcgag aaggtggcgg cggcgtggcg ggacgcggcg gagggggcgt gaaggtcgcc
     2641 ctcgtccacg actggctgac cggcacccgc ggcggggaga aggtcctgcg cgagctggcc
     2701 cgcctcttcc ccgaggcgcc gctcttcacc ctcttccact tcccgggatc ggtcccggcg
     2761 gaggtggagg cgcgggaggt ccggacgacc tggctccagc gcgtcgtctc gccccgccgc
     2821 gactaccgct cgctcctccc gctctacttc gtggcgtgcg aggggtggga cctctccgga
     2881 ttcgaactcg tcctctccac gtcccactgc gtcgcgaagg ccgcgaagcg cggcccgcgc
     2941 gcgttccacc tctgctactg ccacaccccc gtccgctacc tccacgacca gttcgacgac
     3001 tacctgcgcg gtcgcggccc cctcgcgcgg gccgcggcgc gcctcgtccg cgcgccgctc
     3061 cgcgcgcgcg acgtggcgac ggtgccgcgc gtcgacgcat tcctcgcgaa ctccgagaac
     3121 gtgcgggagc ggatcgcgcg cctctacggg cggacggcgc gcgtcgtccc ggcgccggtc
     3181 gacgaccgct tctactcccc ggcgccccgg gagcggcggg ggctcctcgt cgtctcggcc
     3241 ctcgccccgt acaagcgcgt cgacgacgcg atcgcggcct cggcgcggct cggcctcccg
     3301 ctcaccgtcg ccggcttcgg gcccgaggag cggcgcctcc gcgccctcgc cggaccgaac
     3361 gtcacgttcg ccggctcccc gtccgacgag gtcctgcgcg agctctaccg gtcggccgag
     3421 ctcgtcctga tgccgggcga ggaagacttc gggatcgtgc ccctcgaagc ccaggcgtgc
     3481 gcgacgcccg tcgtcgcgct cgggaagggg ggcgccctcg agacggtcgt cgacggcgcc
     3541 accggggtcc tctatccctc ccccgggccc gagccgctcg cgagagcgat cgacctcgcc
     3601 cgctccacgc cgttcgacgg cgcgcggctc gtcgcgaacg cggcgcgctt ctccgaggcg
     3661 gccttccggg cccgattcct cgacgccgcg cgcgcggccc tcctcgaggc gggacggtcc
     3721 gacctcgcgg aggggctccc ccgcgccgaa ccgtagaatc cgcccggtga ccgcgtgatc
     3781 cagaaccagg cccggcggct ccgagcgacc ttcctcgcgt ccgacgtcgc ggccacgtgc
     3841 gcggcgctcc tcgccgctta cgtcgtccgg ttcgagacgg tgtggccgac gccgctcggc
     3901 gtccagccgt tcgggaacta cctccggctt ctcccggtcc tcgcgctcct ctggccggcc
     3961 gtcttctact tccaccgtct ctacccgctc cgccgggacc gctcgttcgt cgacgaagcg
     4021 ctcgcggtcc tcatcgccgc ctcgctcgcg acgctcctcc tcgtcggcct cctctccttc
     4081 tcccgcgctt tctcgctgcc gcggacgctc ctcgtcctct tcctcgggct ggacgtcctc
     4141 ttcgtctcgc tcggccgctt cgcgatctgg cgctacctcg agaaggtctg gagcgccggg
     4201 gtcggggttc gccgcgccct cgtcgtcggc gcgggcgagg cgggccgcgc cgtggcggac
     4261 aagctcctcg accaccccgc ggccggcatc aagccggtcg gcttcgccga cgacgacccg
     4321 gagcgccggg gcgccgagca tcgcgggctt cgtgtcctcg ggacgacggc cgacgtccgg
     4381 gcgctgctcg agtcgcacca ggtcgacacg ctcttcctcg ccctcccggt cgacgcctac
     4441 cggacgatgc tccggatcct gaaggacgtc ggcaacgaga tggtcgacct ccggttcgtg
     4501 cccgacctct tccagttcgt cacgttcaag gcgggggtcg aggacttcga cggcctcccc
     4561 gtcatcaacc tcacgcagcg gcctctcgag gggtggaact ccctcgtgaa gcgctccatg
     4621 gacgtcgtcc tctccgcggg cgggctcgtc gccctttccg tcctcctccc gttcgtcgcg
     4681 ttcgcgatct ggctcgagga ccgggggccg atcttctaca cgcaggagcg gatggggctg
     4741 gacggccgcc tcttccgcat cctgaagttc cggtcgatgc gcgtcggcgc ggaggacgcg
     4801 accggggccg tctgggcgct cgagggcgac tcgcgaagga cgcgcgtcgg agcgttcctc
     4861 aggagcacgt cgctcgacga ggtgccccag ctcgtgaaca tcctccgggg cgacatgtcg
     4921 ctcgtcggcc cgcgtcccga gcgccccgag ttcgttcgcg agttcaagga gaagtacccc
     4981 cagtacatgc tgcggcaccg cgtgaaggcg ggcctcacgg gctgggccca ggtgcacggc
     5041 tggcgcggga acacgagcct ttcgaagagg atcgagcacg acctctacta catcgagaac
     5101 tggagtctcg cgctcgacct ccgcatcctc tggctgaccg cgacccgcag cttccgcgac
     5161 gagaacgcct actgacgccc gggcgccggg ctacttctcc cggagccgga tcgtcccctt
     5221 gtaggtttcc gccacgaggc gcggcccgga gcgcgtctcc tcgatctcgg cgacgagccg
     5281 gccgcgccgg ccgatctcct cgtcgagtgc gacgcgggag cccttcggga ggacgacctc
     5341 gatctccccc ttgtacgtct cgacgcgcag gtcgccggcg acccgcgcga gctcggcccg
     5401 gacgtcgccc ttgtaagtct cgacctcgag gcggcccgag agaccggaga ggcgcgcgac
     5461 ccccttgtag ctctcgaccg agacgtcccc gccgagcccc tcgaccgaga tgcgcgactt
     5521 gtagtcggaa aggtcgaggg tggcgccctg cgggaggcgg atgtcgtact cgacgaacgg
     5581 ccgggacgag cagtcgtcgc ggaaggagaa ccagatcttc ggcaggtctt cgtagtcgga
     5641 gacgacgcgc acctcgcggc cgcccccctc gatccgcacc tgcgtccgct cgacgagctc
     5701 ggccgcccgg tcgcattcgc cgtccggcct cacgaccgcc cggatcgacg cctccgcgcg
     5761 gtcccaggcg acgaccgaca cccgcccctt gtacgtgtcg atcgcgagcc ggccgtccgc
     5821 ggcgagcggc agcgtcttct cgacgacctt cgatacgggc tcggcggccg gcgcggagcc
     5881 ggcgacgagg agcaagaggg caaggacgac ggggacggat cggcgcatgg gtcctcctcg
     5941 gggttcgttc tccgtcggat acggagaaca gccccccgcg gttccgcgcg tcaggtgcgg
     6001 gaacgcgcgg cgcgggcctc gcgaagaagg gccgtgaggc gttccgcctc ggccggagac
     6061 gtcggccgcc ctccgaacga ctcggcgacg taggcgcgga cgatctcgcg ggacggggcg
     6121 cccacgccga ggcggtccgc cgtggagaga gtctccgccg gcgcggaggc gggcgtcagg
     6181 tcggcgcccc gccgccggag gagccgttgg agccgcctgt aggacgcgga ggcgggcggg
     6241 agcccccg
//
LOCUS       DAOTEZ010000250         6236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_8984_length_6236_cov_5.390552, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000250 DAOTEZ010000000
VERSION     DAOTEZ010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6236
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8984_length_6236_cov_5.390552"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="P5164_13010"
     CDS             <1..929
                     /locus_tag="P5164_13010"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HRY44856.1"
                     /translation="QEANRPTRTVIQKLSYASAAAAAGTAKKVMSPRGDIFVDERSNL
                     LIIKELPEYLPTVLDLVKSLDTPVPQVMIEARIVEATRTFSKRLGIDWGFLGVSDQAH
                     GNTTGMVFPNSAEVEGAVSLPNGNQILRASLANVLDTFRLDVALSAAEARGLVKIVSS
                     PKILTQTNVAASVQSGFQIPVQTTVNNTTTVLYIDATLRLDVTPQVTAEGTVILDIKI
                     QRREPAPGVAITGGNNVPLVTRDAQTRLMVRDGGTGMIAGIFKLSANDGQNMIPGLWK
                     IPLLGGLFRNKTMDETTDELMIFITPRVMKAL"
     gene            952..1533
                     /locus_tag="P5164_13015"
     CDS             952..1533
                     /locus_tag="P5164_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44857.1"
                     /translation="MKNRFPLAVALLAGVLAVAGCSSNTQGDDASPVYLSVSYTLLPE
                     SWNVGSGLPLQFDTVELKSNLKNPTGGSSSFLDTRLDDYVVEWKRIDGGTKAPETETF
                     PGNVLIPAGGTSTLSNYPHMTRTALLKAPLDQLQPYNGGIDRETGNSEIRCRATVTYR
                     GRTLSGQPVRGVGSFGLTFLYSAATQTIVARVD"
     gene            1577..4525
                     /locus_tag="P5164_13020"
     CDS             1577..4525
                     /locus_tag="P5164_13020"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HRY44858.1"
                     /translation="MRRTLQPLFAATVGLSLLLAGCSGESPTSPKPPAGGGNDGGSCT
                     VTIALDATGVTPMAGTAIILRATVRKGGALVPDGTSVTFTTDFGFFLETGLPSVSKVT
                     QNGFADVTLGATSAGLAKVKAAFECGTAEKAIDYQPVPANGPFISSISPTSGSCAGGD
                     VVTINGGRFGTDTANVQVLFGGKPASVQGLTETRIEVLTPARTLANPLVPEAVDVVIR
                     FFSGDVPLGDITSPKAFTYYCVDPNKRLTVTSVNPNSGKPEGGQQVVVTGTNFLPSPT
                     SSVATTRVTFGAAPASVVSVSGTQITVQTPRRVLANPAVPETVDVAVTADLGLVSQQS
                     GLLPQAFTYRADGGAGTCTGVPGLFIATVLPEDPTNAGSPDGGDIVVIQGGGFTAGGT
                     STTTDRTAVYFGGNQGVTLSVTDSQIRVTTPRRVLASPDKPETVDVRVLVDAGGPREA
                     CAQAVKAYTYFPGGFLEPVITSISPSAGPNDASTRVTIFGRNFKLPMQVFVGGVEASV
                     VEIKASQIIFLTPLATGPNSALAGQTVDVVVRDPYAGKEYTSPVRFRYYSCPTLNSVV
                     PSSASWNQSTVVTIAGQGFEEPVEVTFESAGLLLRPQVTSVSSSLITLVMPAIDPQLG
                     GGPSCANVPGTLTISFPALASASCDTLDTPFIYRIDAMTAVSATPTQLNQAGGPFGSP
                     LGGAPATITVTGTNFADPMTVEVFGGGVSIPINNAIVANAGQLSFTAPAIPDTALNEQ
                     PCVPSGGTTVTGTRYVATSFGIRLRNARTGCTVDLPNILIYNPADTACRVAASITTAA
                     LPAATLCTAYAGTTATATGGTTPYTWSATGLPPGMTIDALTGAISGTPQLATAGAGGT
                     ISVPVTLSVTDATPTTTSRTIGLQLIDPNGPLAITAPPAATVPLAGGPVGTFTATPNP
                     TPGAFSPITWTVSPATGFNPPAPGQTLALVSDGTLPTGPKTIQVTATDTPSCGGAAHT
                     RTISYTFTVQ"
     gene            4620..6116
                     /locus_tag="P5164_13025"
     CDS             4620..6116
                     /locus_tag="P5164_13025"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY44859.1"
                     /translation="MGLFSRTLSRIDEIKRSLTRETGPRQFLAVADEHRRQGRPREAI
                     DILESGLAQNPSSVAGYVALGRLFQQTAQLDRAIDSFQSALKLDPQNLVAIRQLADVH
                     LARGEKIEAIKRLKLFRGLSPGDREVAELIRQLDVELAPPPSPKSGLRIPVPAVPKAP
                     PPGGPVATGTPKAEPLPAAFVPDDVSASASPSPAEAIAPHVAALPDLSAPGAVSDPSE
                     AVAGPEPERLAEPAEAAPVPFPGEEPLPPAAPPSFPAASSIEAGPALDSLSEGAGAEV
                     PSEPAYAPALSEPVVAPFPAAPEPAPLPVPAATETLARLLRAQGHLGRAREAYLELAR
                     TEPDAEKARRYADAAAEIEAARSASVRGRLEQWVEPFARRPRPTDGDLAAAVEEVVER
                     LAPAAALVTDLEGVPVVTVGPRHEAEAMESLSAELTAFWKNVRRARAEVGEGELRSLV
                     LRGSAGTASVQAITSEYALLLKTVPGIPTGRIRFEVARAAEQLRPALL"
BASE COUNT          978 a         2372 c         2027 g          859 t
ORIGIN      
        1 cccaggaagc gaaccgtccg acccggaccg tgatccagaa gctctcctac gcgagcgccg
       61 cagccgccgc agggaccgcg aagaaggtca tgtccccgcg cggcgacatc ttcgtcgatg
      121 agcggtcgaa cctcctcatc atcaaggagc tccccgagta cctccccacg gtcctcgacc
      181 tcgtcaagag cctcgacacg cccgtgccgc aggtcatgat cgaggcgcgg atcgtggagg
      241 cgacgcgcac cttcagcaag cgcctcggca tcgactgggg gttcctgggc gtctccgacc
      301 aggcccacgg gaacacgacc ggcatggtct tcccgaacag cgccgaggtg gaaggcgcgg
      361 tctcgctccc gaacgggaac cagatcctcc gggcgagcct cgcgaacgtc ctcgacacgt
      421 tccggctcga cgtcgcgctg agcgccgccg aggcgagggg cctcgtcaag atcgtctcct
      481 cacccaagat cctgacgcag acgaacgtcg ccgcgtcggt ccagtcgggc ttccagatcc
      541 cggtccagac gacggtcaac aacacgacga cggtcctcta catcgacgcc acgctccgcc
      601 tcgacgtcac cccgcaggtc accgcggaag ggaccgtcat cctcgacatc aagatccagc
      661 gccgcgagcc ggcgcccggc gtcgcgatca cgggaggcaa caacgtcccg ctcgtcacgc
      721 gcgacgcgca gacccgcctg atggtccggg acggcgggac agggatgatc gcgggtatct
      781 tcaagctctc ggccaacgac ggtcagaaca tgatcccggg cctctggaag atccccctcc
      841 tcggaggcct cttccggaac aagacgatgg acgagaccac agacgagctg atgatcttca
      901 tcacgccgcg cgtgatgaag gcgctctgag gccgcgacac ggagccgaac gatgaagaac
      961 cgattccccc tcgccgtcgc cctcctggcc ggagtcctcg cggtggccgg ctgctcgtcg
     1021 aacacgcagg gagacgacgc ctctcccgtc tacctgtccg tcagctacac gctcctcccg
     1081 gagtcgtgga acgtcggaag cggcctgccg ctccagttcg acacggtcga gctcaagtcg
     1141 aacctgaaga acccgacggg cggctcgtcg agcttcctcg acacccgcct cgacgactac
     1201 gtggtcgagt ggaagcggat cgacggcggc acgaaggccc cggagacgga gacgttcccg
     1261 ggcaacgtgc tcatcccggc cggcgggacc tcgacgttgt cgaactaccc ccacatgacc
     1321 cgcacggcgc tcctcaaggc cccgctcgac cagctccagc cgtacaacgg cgggatcgac
     1381 cgcgagacgg gaaacagcga gatccgatgc cgggcgaccg tgacctaccg gggccggacg
     1441 ctctccggcc agcccgtccg gggcgtcggc agcttcggcc tcaccttcct ctacagcgcc
     1501 gcgacgcaga cgatcgtcgc gcgggtggac tgaggacggc atgtcgaagg tgcgtggaaa
     1561 ctccgaggtg aaagcgatgc gccgaaccct ccagcccctc ttcgccgcga ccgtgggcct
     1621 ttccctgctc ctcgccggct gctccggcga gtcgcccacg tcgcccaaac ccccggccgg
     1681 cgggggcaac gacggcggca gctgcacggt gacgatcgcg ctcgacgcga ccggcgtcac
     1741 cccgatggcc gggaccgcca tcatccttcg ggcgacggtc cgcaaggggg gagccctcgt
     1801 ccccgacggg acctcggtca ccttcacgac cgacttcggc ttcttcctcg agacggggct
     1861 cccgagcgtc tcgaaggtga cgcagaacgg cttcgcggac gtgaccctcg gcgcgacctc
     1921 cgcaggtctc gcgaaggtga aggcggcgtt cgagtgcggc acggcggaga aggcgatcga
     1981 ctaccagcct gtccccgcga acggcccctt catctcgagc atcagcccga cctccgggag
     2041 ctgcgcggga ggggacgtcg tcacgatcaa cggcggacgc ttcggcaccg acaccgcgaa
     2101 cgtccaggtc ctcttcggcg gcaagccggc ctcggtccag ggcctcaccg aaacccggat
     2161 cgaggtgctg acgccggccc gaacgctcgc gaacccgctc gtccccgagg cggtcgacgt
     2221 cgtcatccgt ttcttctcgg gcgacgttcc cctcggcgac atcaccagcc cgaaggcctt
     2281 cacgtactac tgcgtcgacc cgaacaagcg gctgaccgtc acgtcggtga acccgaactc
     2341 gggcaagccg gaggggggac agcaggtcgt cgtcacgggc acgaacttcc tcccttcgcc
     2401 gacgagctcg gtggccacga cgcgcgtcac gttcggcgcc gcgccggcct ccgtcgtctc
     2461 cgtcagcggg acgcagatca ccgttcagac tccgcgtcgg gtcctcgcga accccgcggt
     2521 ccccgagacg gtcgacgtcg cggtgacggc cgacctcggc ttggtctccc agcagagcgg
     2581 cctccttccg caggcgttca cctaccgcgc cgacggcggc gccgggacct gtacgggagt
     2641 gccgggcctg ttcatcgcga cggtcctccc ggaggacccg acgaacgcgg gcagcccgga
     2701 cggcggcgac atcgtcgtca tccagggcgg cggtttcacg gcgggaggca cgagcaccac
     2761 cacggaccgg acggccgtct acttcggcgg caaccagggc gtcacgctca gcgtcaccga
     2821 ctcccagatc cgggtgacga ctccccgccg cgtcctcgcc agccccgaca agccggaaac
     2881 cgtcgacgtc cgcgtcctcg tcgacgcggg cggcccgcgc gaggcgtgcg cccaggccgt
     2941 caaggcctac acctacttcc ccggcggctt cctcgagccg gtcatcacct cgatctcccc
     3001 gtccgcggga ccgaacgacg cctccacgcg cgtcacgatc ttcggccgga acttcaagct
     3061 cccgatgcag gtcttcgtcg gcggcgtcga ggcctcggtc gtcgagatca aggccagcca
     3121 gatcatcttc ctgacgcctc tcgccaccgg accgaacagc gcgctggcgg gccagacggt
     3181 cgacgtcgtc gtccgtgacc cgtacgccgg caaggagtac acgagcccgg tccgcttccg
     3241 gtactactcc tgcccgacgc tcaacagcgt cgtcccgtcg tcggcgtcgt ggaaccagtc
     3301 gacggtcgtg acgatcgccg gccagggctt cgaggagccg gtcgaggtga cgttcgagtc
     3361 ggccggcctg ctcctgcgtc cgcaggtcac gtcggtctcc tcctcgctca tcactctcgt
     3421 catgcccgcg atcgacccgc agctcggcgg cggaccgtcg tgcgccaacg tcccgggcac
     3481 gctcacgatc tcgttccccg cgctcgcctc ggcgtcctgc gacacgctcg acacgccgtt
     3541 catctaccgg atcgacgcga tgacggcggt cagcgccacc ccgacgcagc tgaaccaggc
     3601 gggcggcccc ttcggctcgc cgctcggggg cgcgccggcg acgatcaccg tcacgggaac
     3661 gaacttcgcg gacccgatga cggtcgaggt cttcggcggc ggcgtcagca tcccgatcaa
     3721 caacgccatc gtggcgaacg cgggccagct cagcttcacg gccccggcca tccccgacac
     3781 ggcgctgaac gagcagccct gcgtcccgtc gggtggcacg accgtcaccg ggacccggta
     3841 tgtcgccacg tcgttcggca tccgtctccg gaacgcccgg acgggctgca cggtcgacct
     3901 tccgaacatc ctcatctaca acccggccga caccgcctgc cgcgttgcgg cttcgatcac
     3961 gacggccgct ttgcccgccg cgacgctctg caccgcctac gccgggacga ctgcgacggc
     4021 gacgggcggg acgacgccgt acacctggag cgcgaccggc ctgcctcccg gaatgacgat
     4081 cgacgcgctc accggagcga tctccggcac cccgcagctg gcgacggcgg gcgcgggcgg
     4141 gacgatctcg gtccccgtca cgctcagcgt caccgacgcg acgccgacca cgacgagccg
     4201 gacgatcggc ctgcagctca tcgacccgaa cgggccgctc gcgatcacgg cccctccggc
     4261 cgcgacggtt cccctcgcgg gcgggccggt cggtacattc acggccaccc cgaatccgac
     4321 cccgggagcc ttctcaccga tcacctggac ggtgagcccc gccaccggtt tcaacccgcc
     4381 cgctccgggc cagactctgg cgctcgtgtc ggacgggacg cttccgacgg gacctaagac
     4441 gatccaggtc actgcgaccg acacgccctc gtgcggcggc gcagcccata cgcggacgat
     4501 cagctacacc ttcacggttc agtaggcttc tcgcagacgt ctccacccgg ctcgggccgc
     4561 ccctcggggc ggcccgagct ttttccgccg cccgctttgc cgtacactga ccgggaccga
     4621 tgggcctctt cagcaggacg ctgtctcgca tcgacgagat caagaggagc ttgacccggg
     4681 agaccgggcc gcggcagttc ctcgccgtcg cggacgagca tcgcaggcag gggcgcccgc
     4741 gcgaggcgat cgacatcctc gagtcggggc tcgcgcagaa tccttcctcg gtggcgggct
     4801 acgtcgccct cgggaggctc tttcagcaga cggcccagct cgacagggcg atcgactcct
     4861 tccagtcggc gctgaagctc gatccgcaga acctcgtcgc gatccggcag ctcgcggacg
     4921 tccacctcgc ccgcggcgag aagatcgagg cgatcaagcg gctgaagctc ttccgcggcc
     4981 tcagcccggg agaccgggag gtcgcggagc ttatccgcca gctcgacgtc gagctggccc
     5041 cacctccttc gccgaagtcg ggactccgga tccccgtgcc ggccgtcccg aaggcgcccc
     5101 cgccgggcgg gccggtcgcg acgggaaccc cgaaggcaga gccgcttccg gcggccttcg
     5161 tcccggatga cgtctcggcg tcagcctccc cgtctcctgc cgaagcgatc gccccgcacg
     5221 tcgcagcgct tccggacctg tccgccccgg gggccgtctc ggacccctcg gaggcggtcg
     5281 ccggacccga gcccgagcgg ctcgcagagc cggctgaagc cgcgcccgtc ccgttcccag
     5341 gggaagagcc tttgcccccg gcggcgccac cgtcgttccc ggcggcctcg tcgatcgagg
     5401 ccggtccggc gctcgactca ctctccgagg gcgccggggc ggaggtcccg tcggagccgg
     5461 cttacgcccc ggctctgtcc gagccggtcg tcgctccgtt ccctgcggcc ccggagccgg
     5521 cgccgcttcc cgtgcccgcc gccaccgaga cgctggccag gctgctgcgt gcccagggcc
     5581 acctcgggcg cgcccgcgag gcctatctcg agctggcccg caccgagccc gacgccgaga
     5641 aggctcggcg atatgccgac gccgccgcgg agatcgaggc ggcgcggagc gcgtcggtgc
     5701 gcgggcggct cgagcaatgg gtcgagccct tcgcacgaag gccccgcccg accgacggag
     5761 acctcgctgc cgccgtcgaa gaggtcgtcg agaggctcgc gccggcggcg gctctcgtga
     5821 ccgacctgga gggagtcccg gtcgtcaccg tcggcccccg acacgaggcc gaggcgatgg
     5881 agagcctgtc agccgagctg acggcgttct ggaagaacgt tcgccgcgcc cgcgccgagg
     5941 tcggcgaggg ggagctccga agcctcgtcc tcagggggag cgccgggacg gcgtcggtgc
     6001 aggcaatcac gtccgaatac gcgctcctcc tgaagaccgt ccccggcatc ccgacgggac
     6061 ggattcggtt tgaggtggcg cgcgccgccg agcagcttcg tccggccctc ctctgagggc
     6121 cgaaccgccc cgcagaagac cgaaccccgc gaagagaagg aaatgaacgt caaggaaatc
     6181 agggaactcg cagatctggt cgctcgccgg ggcctcgcca gcctcgaggt cgagga
//
LOCUS       DAOTEZ010000251         6203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_9042_length_6203_cov_6.322707, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000251 DAOTEZ010000000
VERSION     DAOTEZ010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9042_length_6203_cov_6.322707"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1771)
                     /locus_tag="P5164_13030"
     CDS             complement(<1..1771)
                     /locus_tag="P5164_13030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY44860.1"
                     /translation="MTRPKKPGGAFSGEFLAEAEMLLEEAGCGIDALDADGDDPNPER
                     VNALFRSVHSLKGVAGMVGYAGVADAAHALEALLDDLRMGRVPASPAVRGAVREGLGA
                     LSTLVARVAAGEDAPRLETPLKDRLEGLVRPAEPRGTESLRLPPELDASLSDYERHRA
                     SEAGKRGKALVLVDLDLDFDSFDAGLRNAMSEASAAGELIGTFPGTAADPARMAFRLL
                     VALPPGCDAAALGTRCSARDVVTVSAGRAAPPPSAAPARLPAAVPAESAPPEPGPEAA
                     PQVARGVVRVPLEKVAALVDLAGELALARSALGRSLGQALAGASDRGARFEAQRAFAR
                     LDRLVATLGPAALALRLVPVETLTTRLARAFARTASALGREADFVVQGVETEIDKVLA
                     DELGDPLLHILRNALDHGIESPEERSSAGKPRRGRVALTVAPSGREVVLTFSDDGRGV
                     DAAGVVAKARQTGLLGATDPDPSDPFALLFRPGFSTAREVSEISGRGVGLDVVREKLA
                     ALGGRVSVRSIPGSGTTFEVVVPMTLVLLESLVVRVGEVWFALPGENVRRTLQGAADG
                     VEIVDGRACFRDGDEALPLARLDA"
     gene            complement(1768..2190)
                     /locus_tag="P5164_13035"
     CDS             complement(1768..2190)
                     /locus_tag="P5164_13035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY44861.1"
                     /translation="MTASGPIRRHVLIVDDSGPARLLVRQALEGWARRTGQRVDVVEA
                     ENGFEALRELPRAPIDLVLTDVNMPTLSGLELIRMIRSRPEQSALPVIAVSTERDAED
                     AARARKAGADGYLGKPFDVETFDRVLGEVLGSKAPEEP"
     gene            complement(2187..3047)
                     /locus_tag="P5164_13040"
     CDS             complement(2187..3047)
                     /locus_tag="P5164_13040"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HRY44862.1"
                     /translation="MTAALLAAALLLLSGPERSAAPGRVAVETARYQVEGPEPWRAVR
                     AVEAPGYRVALSSPDGRTLLATVEVDAAPLRDTAPYPVDPRTLPAEARVLLTEPLDAD
                     DELEDLSRILLRGATTQLEAVERVVAWVSKRIRYEPPSLLPESAATCHRSRRGSCVGR
                     SLLAADLLRRGGIPVRQVTGILTARTPSELTDNSQEHFSETISGVRHRWIEVYVPGLG
                     WVPSDPGGLANMLTARHLALPAAPPEGFGLSIVSRGPALAWKARDGTFARPRLRVVEA
                     VDLPPGGRQP"
     gene            complement(3044..3505)
                     /gene="nusB"
                     /locus_tag="P5164_13045"
     CDS             complement(3044..3505)
                     /gene="nusB"
                     /locus_tag="P5164_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRY44863.1"
                     /translation="MSGRRRRARELALQLLYQRDVAGTSPEEMFARTDEYRNAAPEVQ
                     EYASRLVLGTLGRLAELDEMLGKQSDHWRLGRMPVVDRNLLRVALYELLHEDETPDPV
                     VIDEAVEIAKKFSTPSSAPFINGVLDGIRRNLFGAREASPRRSRPAPAGSR"
     gene            complement(3515..3997)
                     /gene="ribH"
                     /locus_tag="P5164_13050"
     CDS             complement(3515..3997)
                     /gene="ribH"
                     /locus_tag="P5164_13050"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRY44864.1"
                     /translation="MNVLEGQLRADGRRFGIVASRTNDIVVSRLLDGALDAFRRLGAA
                     EGAVTVVKVPGSWEIPLALRDLGKVGGFDALVALGAVIRGGTPHFDYVATEVAKGVFQ
                     VGLELDIPITFGVLTCDSLEQALERSGAKGGNKGFEAATAAVELADLRARLRAGAPVR
                     "
     gene            complement(4005..5066)
                     /locus_tag="P5164_13055"
     CDS             complement(4005..5066)
                     /locus_tag="P5164_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HRY44865.1"
                     /translation="MSTNPKVVVTGSVAFDYLMRFPGKFQDVVVPDRMHRLSVSFLVD
                     EMRRVRGGVAPNIAYGLALFGMTPAIFATAGQDAADYRAWLGEQGCDVSGMKLCDDVF
                     TASFFVSTDLEQNQIATFYAGAMARASELTFRSFEPGSVALALICPSDPTAMRQHAAE
                     CRELGIPFVYDPSQQVARLGGDDLLAGLEGASMLIGNEYELGVIEKKTGLSEAELAAK
                     VPVLVITRGEEGATIALRGGPPEEPGGARTLRIPAAKLRTEALDPTGVGDAFRSGLVA
                     SRLRGLPWEEAGRLGAVAAVLCLETLGPQPRRWVLDELLERYAESFGAPLAAPLAERL
                     RGAAGAGSGRPATRFSLLD"
     gene            complement(5063..5923)
                     /gene="mtnP"
                     /locus_tag="P5164_13060"
     CDS             complement(5063..5923)
                     /gene="mtnP"
                     /locus_tag="P5164_13060"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HRY44866.1"
                     /translation="MRKRIGIVGGSGLYALPGLTVEEERAIETPFGPPSDAFVLGELS
                     GVPVAFLARHGRGHRLTPSEVSYRANVWGFRSLGCSYLVSSSACGSMKLEYRPTDIVL
                     PDQLLDLTKGRASTFFGEGCVAHVSFSHPVSAHLVEALARAAAETGAVAHRGGTYLCM
                     EGPQFSTLAESRLYRSWGVDVIGMTNATEAKLCREAEIDYATMCLVTDYDCWHEEEAP
                     VSVEAVLAVLRKNAETADRVLGRAVASLDPERPPDCDGAMRHAVLTAPEAIPPAARER
                     LAVLKERYWR"
     gene            complement(5920..>6203)
                     /locus_tag="P5164_13065"
     CDS             complement(5920..>6203)
                     /locus_tag="P5164_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY44867.1"
                     /translation="KTRKFRFFQPDWYFSIDTQKRDVTAYRLDRMSGPPRIMEWPVTV
                     VPADPLTAEDGAFLEACRTRTEPAVSGEAGRAALKLALDVQEEVRRRVG"
BASE COUNT          855 a         2305 c         2260 g          783 t
ORIGIN      
        1 gcgcgtcgag gcgcgccagc gggagggcct cgtcaccgtc gcggaagcag gcccgtccgt
       61 cgacgatctc gacgccgtcc gcggcgccct ggagcgtccg ccggacgttc tcccccggaa
      121 gggcgaacca gacctcgccc actctcacga cgagcgactc caggaggacg agcgtcatcg
      181 ggacgaccac ctcgaacgtc gttcccgagc cggggatcga tcggaccgag acgcgacctc
      241 cgagggcggc cagcttctcc ctcacgacgt cgagcccgac gccccgcccg gagatctccg
      301 agacctcccg cgccgtcgag aagccggggc ggaagaggag cgcgaacggg tcggaggggt
      361 cggggtccgt cgctccgagg aggccggtct ggcgggcctt cgcgacgacg cccgccgcat
      421 cgacgccccg gccgtcgtcg gagaacgtca gcaccacctc gcgtccgctc ggcgccaccg
      481 tcagggccac gcgcccccgg cgcggcttgc cggccgagga ccgctcctcg ggcgactcga
      541 ttccgtggtc gagggcgttt cgaaggatgt ggagcagcgg gtcgcccagc tcgtcggcga
      601 ggaccttgtc gatctccgtc tcgaccccct ggacgacgaa atcggcttcg cggccgagcg
      661 cggaggcggt cctcgcgaag gcgcgtgcga gccgcgtcgt cagcgtctcc accgggacga
      721 ggcgcagggc cagcgcggcg ggcccgagcg tcgcgacgag gcggtcgagc cgggcgaagg
      781 cccgctgtgc ctcgaagcgg gctccccggt ccgacgcccc ggcgagggcc tgcccgaggg
      841 aacgtcccag cgcggaccgg gccagcgcca gctccccggc gaggtcgacg agcgcggcca
      901 ccttctcgag cgggacgcgg acgactcccc gggcgacctg gggggcggcc tccggtcccg
      961 gctcgggcgg cgcggactcc gcgggcaccg cggcgggcag gcgggcgggc gcggcggagg
     1021 gcggcggggc ggccctgccc gcggagaccg tcacgacgtc gcgcgccgag caccgggtcc
     1081 ccagtgcggc cgcgtcgcag ccgggaggca gcgccacgag gaggcggaac gccatgcggg
     1141 ccgggtccgc cgccgttccg ggaaacgtcc cgatcagctc gcctgcggcc gacgcctcgc
     1201 tcatcgcgtt ccggagcccg gcgtcgaacg agtcgaagtc gaggtcgagg tcgacgagga
     1261 cgagagcctt gccgcgcttc cccgcctcgg acgcacgatg cctctcgtag tccgaaaggg
     1321 aggcgtcgag ctcgggcggc agccgaaggc tctcggtccc gcgcggctcc gcgggacgga
     1381 cgaggccctc gagccggtct ttcagcggcg tctcgagcct cggcgcgtcc tcgcccgcgg
     1441 cgacccgggc cacgagagtc gaaagcgcgc cgaggccttc gcggacggcg ccgcgcacgg
     1501 cgggcgacgc ggggacgcgc cccatccgga ggtcgtcgag cagcgcctcg agcgcgtgcg
     1561 cggcgtcggc gaccccggcg tagccgacca ttcccgcgac gcccttcagg gagtggaccg
     1621 atcgaaagag cgcgttgacc ctctccggat tggggtcgtc tccgtccgcg tcgagggcgt
     1681 cgatcccgca cccggcctcc tcgaggagca tctccgcctc ggccaggaac tcgccggaga
     1741 acgcgccgcc cggcttcttc gggcgggtca cggctcctcc ggcgccttcg atcccaggac
     1801 ctcgccgagg acccggtcga acgtctcgac gtcgaacggc ttcccgaggt agccgtcggc
     1861 ccccgccttc cgcgctcgcg cggcgtcctc cgcgtcgcgc tccgtcgaga cggcgatcac
     1921 cggaagggcg ctctgctccg ggcgggagcg aatcatccgg atcagctcga ggcccgagag
     1981 ggtcggcatg ttcacgtcgg tcaggacgag gtcgatgggc gcgcgcggga gctcccgcag
     2041 cgcctcgaac ccgttctccg cctcgacgac gtccaccctc tggcccgtcc ggcgggccca
     2101 cccctcgagg gcctgccgaa ccaggagtcg cgcgggtccg gaatcgtcga cgatgaggac
     2161 gtgccgacgg atcggtcccg acgcggtcat ggctgccgtc ccccgggagg caggtccacc
     2221 gcttcgacga cgcggagccg gggccgggcg aacgtgccgt cgcgggcctt ccacgcgagc
     2281 gcggggccgc gggagacgat cgacagcccg aagccttcgg gcggagccgc cggaagggcc
     2341 aggtgtcgcg ccgtcagcat gttcgcgagc cctccggggt ccgacgggac ccatccgagc
     2401 cccggcacgt agacctcgat ccaccgatgc cgcacgccgc tgatcgtctc gctgaagtgc
     2461 tcctgcgagt tgtcggtcag ctcgctcggc gtgcgggcgg tcaggattcc ggtcacctgg
     2521 cggaccggga ttccgccccg gcgcaggaga tcggccgcga ggagcgatcg gccgacgcac
     2581 gagccccgcc ggctccggtg gcacgtggcg gcggactccg ggaggaggct cggcggctcg
     2641 tagcggatcc gcttcgagac ccaggcgacg acccgctcga cggcctcgag ctgagtcgtg
     2701 gctccgcgca ggaggatccg ggagaggtcc tccagctcgt cgtcggcgtc gagcggctcg
     2761 gtcaggagca cccttgcctc ggccgggaga gtgcgcgggt cgaccggata cggggccgtg
     2821 tcgcgcaggg gggccgcgtc cacctcgacc gtcgccagga gcgttcgtcc gtcgggcgat
     2881 gagagcgcga cgcggtagcc cggcgcctcg acggccctca cggcgcgcca cggctccggc
     2941 ccctcgacct gatagcgggc cgtctccacg gcgactcgtc ccggtgcggc gctcctctcg
     3001 ggccccgaga ggaggaggag cgcggctgcc aggagcgccg cggtcatcgg ctcccggccg
     3061 gcgccggcct cgaccgccgc ggcgacgctt cgcgcgcccc gaagaggttg cggcggatgc
     3121 cgtcgagcac gccgttgatg aacggcgcgg aggacggggt ggagaacttc ttggcgatct
     3181 cgaccgcctc gtcgatgacg acggggtcgg gggtctcgtc ctcgtgaagg agctcgtaga
     3241 gggcgacgcg gaggaggttc cggtcgacga ccggcattcg cccgagccgc cagtggtccg
     3301 actgcttccc gagcatctcg tccagctccg cgagccggcc gagcgtgccg aggacgaggc
     3361 gcgaggcgta ctcctggacc tcgggcgcgg cgttccggta ctcgtccgtc cgggcgaaca
     3421 tctcctcggg cgacgtcccg gcgacgtccc gctggtagag gagctggagc gccagctcac
     3481 gggcccgccg tctgcggccg ctcacggcga ccccctaccg gaccggcgcg cccgcccgca
     3541 gccgcgcgcg gaggtccgcc agctcgacgg ccgccgtcgc cgcctcgaac cccttgttgc
     3601 cccccttcgc gccggatcgc tcgagcgcct gctcgagcga gtcgcacgtc aggacgccga
     3661 aggtgatcgg gatgtcgagc tcgagcccga cctggaacac tcccttcgcg acctcggtcg
     3721 cgacgtagtc gaaatgcggg gtgccgcccc ggatcacggc gcccagcgcg acgagggcgt
     3781 cgaagccccc gaccttcccg agatcgcgca gcgccagagg gatctcccac gagcccggca
     3841 ccttcacgac ggtgacggcc ccctcggccg cgccgagccg ccggaaggcg tcgagggcgc
     3901 cgtcgaggag gcgggagacg acgatgtcgt tggtccgtga ggcgacgatg ccgaaccgcc
     3961 ggccgtcggc gcgaagctgg ccctcgagca cgttcatgac cgtctcagtc tagcagagag
     4021 aagcgcgtcg ccggacgccc ggagcccgct cccgcggcgc cccggagccg ctcggcgagc
     4081 ggggcggcga ggggcgcgcc gaagctctcg gcgtaacgct ccaggagctc gtcgaggacc
     4141 cagcgacggg gctgcggccc gagcgtctcc aggcaaagga ccgccgcgac ggcgcccagc
     4201 ctccccgcct cttcccaggg gaggccgcgc aggcgcgagg cgacgagccc cgaccggaac
     4261 gcgtcgccca cgccggtcgg atccagcgcc tcggtccgga gcttcgcggc cgggatccgg
     4321 agcgtccggg cgccccccgg ctcctccggc gggccgccgc ggagggcgat cgtcgccccc
     4381 tcctcgcctc gcgtgatgac gaggacgggg accttcgcgg ccagctccgc ctccgacagc
     4441 cccgtcttct tctcgatcac gccgagctcg tactcgttgc cgatcagcat cgaggccccc
     4501 tcgaggcccg cgagaaggtc gtcgccgccg agccgggcca cctgctggct cgggtcgtag
     4561 acgaacggga tgccgagctc gcggcactcc gccgcgtgct gccgcatcgc cgtcgggtcg
     4621 ctcggacaga tcagggcgag cgcgaccgac cccggctcga acgaccggaa cgtgagctcc
     4681 gaggccctcg ccatcgcgcc ggcgtagaac gtcgcgatct ggttctgctc gaggtccgtc
     4741 gagacgaaga acgacgccgt gaagacgtcg tcgcagagct tcatgccgga gacgtcgcac
     4801 ccctgctccc cgagccaggc gcggtagtcg gcggcgtcct gccccgccgt cgcgaagatc
     4861 gccggcgtca tcccgaagag cgccaggccg taggcgatgt tcggcgcgac accgccgcgg
     4921 acgcgccgca tctcgtccac gaggaacgag accgacaggc gatgcatccg gtccgggacg
     4981 acgacgtcct ggaacttccc ggggaagcgc atcaggtagt cgaacgccac cgagccggtg
     5041 acgacgacct tcgggttcgt gctcatctcc aatacctttc cttcagcacc gcgagcctct
     5101 cgcgggccgc cggcgggatc gcctccggcg cggtcaggac ggcgtggcgc atcgcgccgt
     5161 cgcagtcggg cgggcgttcc gggtcgagag aggcgacggc gcggccgagg acccggtccg
     5221 ccgtctcggc gttctttcgg aggacggcga ggaccgcctc gaccgagacc ggcgcctctt
     5281 cctcgtgcca gcagtcgtag tcggtcacga ggcacatcgt ggcgtagtcg atctccgcct
     5341 cccggcagag cttcgcttcc gtggcgttcg tcattccgat gacgtcgacg ccccaggagc
     5401 ggtagaggcg cgactcggcg agcgtcgaga actgcggccc ctccatgcag aggtacgtcc
     5461 cgccgcgatg cgcgacggcg cccgtctccg ccgccgcccg ggccagggcc tcgacgagat
     5521 gggcggacac cgggtgcgag aacgagacgt gggccacgca cccctcgccg aagaacgtcg
     5581 acgcccgccc cttcgtcagg tcgaggagct ggtccggcag gacgatgtcc gtcggccggt
     5641 actcgagctt catcgagccg caggcggagg aggagaccag gtacgagcat ccgaggctcc
     5701 ggaagcccca gacgttcgcc cggtagctca cctcggacgg cgtgagccga tgcccccggc
     5761 cgtggcgggc gaggaatgcg accgggaccc ccgagagctc tccgaggacg aaggcatccg
     5821 agggaggccc gaacggcgtc tcgatcgccc gttcctcctc gaccgtcagg ccggggagag
     5881 cgtagaggcc cgatccgccg acgattccga tccgcttcct cacccgaccc tccggcggac
     5941 ttcctcctgg acgtcgagag cgagcttcag cgccgcgcgg ccggcctcgc cgctcaccgc
     6001 cggctccgtc cgtgtccggc aggcctccag gaacgccccg tcctccgccg tgagcgggtc
     6061 ggcggggacg accgtcacgg gccactccat gatccggggc ggcccgctca tccgatcgag
     6121 ccggtaggcc gtcacgtcgc gcttctgggt gtcgatcgag aagtaccagt cgggctggaa
     6181 gaagcggaac ttccgcgtct tct
//
LOCUS       DAOTEZ010000252         6190 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_9071_length_6190_cov_5.708883, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000252 DAOTEZ010000000
VERSION     DAOTEZ010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6190
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9071_length_6190_cov_5.708883"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2241
                     /locus_tag="P5164_13070"
     CDS             <1..2241
                     /locus_tag="P5164_13070"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRY44868.1"
                     /translation="VGQDASRGNPFPQNAVQEFRVITQNFKAEYQKASSAIITAVTKS
                     GGNELHGDVFVYYQNKNLVAIDGVTQRRADANNTEAVKPEYERWQPGVSLGGPIVKDK
                     LHFFASYEGNVQDREYEVFRGTNMNWPAAVRDQFKPWEGLYTSPFRSTLFFGKVTASL
                     AENSHLDVSGDLRHETDIRGFGGQTSYQAAENVKNDVWTVRGKHTAVLGSYLNEATAS
                     FQRFKWNPVPQDNSLPGLNYDGLMRVGSKDTQQNFTQDRLAIRDDVTLMNLTWNGDHV
                     VKGGVTYDYLKYHVVKQLNGNPVFNFRNSTFVTTPGDMPYNAIYGVGDPDLSTNNTQF
                     GLYVQDDWRITSRFTANVGLRWDFETDMLNNDYETPADIRAAFAGIYPSNYLTDGTQR
                     DTFYGAIQPRLGLSWDVRGDGKTVLHAGYGKYYDRTLYNDILDEKFRLQYKVTTFWFS
                     PDGGPMDGRPAIKWDPRYLSLTGLQGLLASGEAPQPELFFLANDTRPPSSDQWSLGVR
                     HDFGPFNAAVTYTGVKSKDQLTWTCGIKNADGTCNWGARPAPGYGFSMLSRSKEAWFD
                     SVQVVLDKPFSATAGWGAFLSYVWSDAEQTGNDLFSWGKLDPVNGIRQRSAGSQEHQI
                     TVSGIVGLPWEVRASTLVTLGSGYPFGGTDCSAGWDKCVDHVGAGDPPKWTQSIDVRL
                     EKRFALGGSYDVNVFAEAINVFNYTNEKGYDGWQPALPEINTNYGKPNEGYNPRRIQL
                     GASFGF"
     gene            2252..3775
                     /locus_tag="P5164_13075"
     CDS             2252..3775
                     /locus_tag="P5164_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HRY44869.1"
                     /translation="MNPRPVSRAVATLLLAALAAGCGSPSSARAGGEPASPARVVPSP
                     DTGEVPAPTRVLDPLVEELQHRTFRWFWETANPQNGLVPDRWPAPSFSSVAAVGFGLS
                     AYLVGVERGWVSREEARERVLVTLRFLWNAPQGPAAAGMTGYKGFFYHFLDMETGKRW
                     GKDVELSSIDTTLCLAGALSAGLYFDRDDPAEAEVRDLAKRLYERVDWRWMQPRPPRV
                     AMAWLPETGFGPADWHGYDESMVLYLLALGSPTHPATDGAWETFVSTYKWAEHEGQTY
                     VNFAPLFGHQFSHVWIDFRGIRDVFLREKGIDYFENSRRAVLAQRAYAIRNPEGFLGY
                     GPDVWGLSACDGPADVTRTVDGKARRFVTYGARGVSSFWSHDDGTITPMAAAASLPFA
                     PEVVLPAIREMKRLHGEHLWGEYGFFDAFNPTFRFTDVPTPLGKVVPEAGWYATNYLG
                     IDQGPIVLMLENQRSGLLWRLMKRSPAIETGLRRAGFTGGWLEEGTAPARSGEAAIR"
     gene            3772..5052
                     /locus_tag="P5164_13080"
     CDS             3772..5052
                     /locus_tag="P5164_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004426953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRY44870.1"
                     /translation="MRALACGLLLAAATACARGGERGEAVRLWAFGREGEVVRELVPE
                     LEKRHPGLRVEVQQIPWTAAHEKLLTAYVGRELPDVAQMGNTWIPEMTALDAIEPLDA
                     RVAASAAVQPGDYFPGIWETNVLDGTLYGVPWYVDTRLVFYRTDLLAAAGVRDFPKSW
                     SAWRAAMEAVARRGGPGRFGLLLPTNEWAQPVALAVAAGAELLSDGARHGAFRAPEFR
                     RAAEFFVGLYRDGLAPPLANTQVANLYQQMAAGDFAMYVTGPWNLGEFRRRLPASLQG
                     SWTTAPLPAPDGMPWPGVSLAGGSSLVVFRSSSRKDDAWKVVEFLSEPATQKRFWQLC
                     GDLPARRSAWDEPALATDARTAPFRLQLEHVRATPKVPEWEQIAAKVWERLEPVARGT
                     RDLDAVLTALDADVDAILAKRRWLLERKAAEGRP"
     gene            5049..5954
                     /locus_tag="P5164_13085"
     CDS             5049..5954
                     /locus_tag="P5164_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRY44871.1"
                     /translation="MRRLSSWRAREARAGLLLVAPGLLLLALFLALPVVAALLLSLTD
                     FDLYAIGDPSAARVVGLGNYARLLHDPVFWKALGNTFFFVVFGGPLTVSLSLGAALLL
                     NHRLVRFPGLFRTIYFAPVVTTLVAVAAVWRYLYHPRFGLLNAALGFFGIGPVDWLGD
                     PHWAMPAIVLMAAWKNFGANMIIFVAGLKAIPESLYEAARIDGAGPIQRFRKITVPLL
                     APTFLFVGLTTTIGYFQLFAEPYVLSGGEGGPMNATLSVALFMYKQGFRWWSIGSAAS
                     IAFVLFAVILAATLLQRRLQKGGEA"
     gene            6005..6190
                     /locus_tag="P5164_13090"
                     /pseudo
     CDS             6005..6190
                     /locus_tag="P5164_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
BASE COUNT          919 a         2227 c         2115 g          929 t
ORIGIN      
        1 gtcggccagg acgcgagccg cgggaacccg ttcccgcaga acgccgtcca ggagttccgg
       61 gtcatcacgc agaacttcaa ggccgagtac cagaaggcct cgagcgccat catcacggcc
      121 gtcacgaagt cgggcggcaa cgagctccac ggagacgtct tcgtctacta ccagaacaag
      181 aatctcgtcg ccatcgacgg cgtcacgcag cgccgcgcgg acgcgaacaa caccgaggcg
      241 gtcaagcccg agtacgagcg ctggcagccg ggagtcagcc tcggcgggcc gatcgtgaag
      301 gacaagctcc acttcttcgc ctcctacgag gggaacgtcc aggaccgcga gtacgaggtc
      361 ttccgcggca cgaacatgaa ctggcccgcg gccgtccgcg accagttcaa gccgtgggag
      421 gggctctaca cgagcccgtt ccgctcgacc ctcttcttcg ggaaggtcac ggcctcgctg
      481 gccgagaact cgcacctcga cgtctccgga gacctccggc acgagacgga catccggggg
      541 ttcggggggc agacgagcta ccaggcggcg gagaacgtga agaacgacgt ctggacggtc
      601 cgcggcaagc acaccgcggt cctcgggtcg tacctgaacg aggcgacggc cagcttccag
      661 cgcttcaagt ggaacccggt cccgcaggac aacagccttc ccggcctgaa ctacgacgga
      721 ctcatgcggg tgggcagcaa ggacacgcag cagaacttca cgcaggaccg gctcgcgatc
      781 cgggacgacg tgacgctgat gaacctgacg tggaacggcg accacgtcgt caagggcggc
      841 gtcacctacg actacctgaa gtaccacgtc gtcaagcagc tgaacgggaa cccggtcttc
      901 aacttccgca actccacgtt cgtcacgacc ccgggcgaca tgccgtacaa cgcgatctac
      961 ggcgtcggcg atcccgacct ctcgacgaac aacacgcagt tcggcctcta cgtccaggac
     1021 gactggcgga tcacctcccg cttcacggcg aacgtcggcc tccgctggga cttcgagacc
     1081 gacatgctca acaacgacta cgagacgccg gccgacatcc gcgcggcgtt cgcggggatc
     1141 tacccgtcga actacctgac cgacggcacg cagcgcgaca cgttctacgg cgcgatccag
     1201 ccgcgcctcg gcctttcgtg ggacgtgcgc ggcgacggga agacggtcct ccacgccggc
     1261 tacggcaagt actacgaccg gacgctctac aacgacatcc tcgacgagaa gttccggctc
     1321 cagtacaagg tcacgacgtt ctggttctcg cccgacggag gcccgatgga cgggcgtccg
     1381 gcgatcaagt gggacccgcg atacctgagc ctgacgggcc tgcagggcct cctcgcgagc
     1441 ggcgaggcgc cgcagcccga gctcttcttc ctggccaacg acacgcgccc gccctcttcg
     1501 gaccagtgga gcctgggggt ccggcacgac ttcgggccgt tcaacgccgc ggtcacctac
     1561 acgggcgtga agagcaagga ccagctcacc tggacgtgcg gcatcaagaa cgcggacggg
     1621 acctgcaact ggggagcccg cccggcgccc ggatacggct tctcgatgct ctcgcggtcg
     1681 aaggaagcct ggttcgactc cgtccaggtc gtcctcgaca agcccttctc ggccaccgcg
     1741 ggctgggggg cgttcctttc gtacgtctgg tcggacgccg agcagaccgg caacgacctc
     1801 ttcagctggg gcaagctcga cccggtgaac ggcatccggc agcggagcgc gggctcgcag
     1861 gagcaccaga tcacggtttc gggcatcgtg gggctcccgt gggaggtgcg cgcgtcgacc
     1921 ctcgtcacgc tcggctcggg ctacccgttc ggcgggaccg actgctccgc cggctgggac
     1981 aagtgcgtcg accacgtcgg cgcgggcgac ccgccgaagt ggactcagag catcgacgtc
     2041 cggctcgaga agcgcttcgc cctgggcggc tcgtacgacg tgaacgtctt cgccgaggcg
     2101 atcaacgtct tcaactacac gaacgagaag gggtacgacg gctggcagcc ggcgctcccg
     2161 gagatcaaca cgaactacgg caagcccaac gaaggctaca acccgcgccg gatccagctc
     2221 ggcgcgagct tcggattctg aggaggaccc ggtgaacccg cggcccgtgt cccgagccgt
     2281 cgcgacgctc ctgctggcgg cgctcgccgc gggctgcggg tccccgtcct ccgcgcgcgc
     2341 aggaggggag ccggcctctc ccgcgcgcgt cgtcccgtcc ccggacacgg gcgaggtccc
     2401 ggctcccacc cgggtcctcg accctctcgt cgaagagctg cagcaccgga ccttccgctg
     2461 gttctgggag acggcgaacc cgcagaacgg actcgttccc gaccgctggc ccgccccgtc
     2521 attctcgagc gtcgccgccg tcggcttcgg cctgtccgcg tatctcgtcg gcgtcgagcg
     2581 aggctgggtg agccgggagg aggcgcggga gcgcgtcctc gtcacgctcc gcttcctctg
     2641 gaacgccccg cagggaccgg cggccgcggg gatgacgggc tacaagggct tcttctacca
     2701 ctttctcgac atggagacgg ggaagaggtg ggggaaggac gtggagctct cctccatcga
     2761 cacgacgctc tgcctcgccg gggccctctc ggcggggctc tacttcgacc gcgacgaccc
     2821 ggcggaagcc gaggtccgcg atctcgcgaa gaggctctac gagcgggtcg actggcgatg
     2881 gatgcagccg cgtccgcccc gcgtcgcgat ggcgtggctg ccggagaccg gcttcggccc
     2941 ggccgactgg cacggctacg acgagtcgat ggtcctctac ctcctcgcgc tcggatcacc
     3001 gacgcatccg gcgacggacg gggcgtggga gacgttcgtc tcgacctaca aatgggcgga
     3061 gcacgagggg cagacctacg tcaacttcgc ccccctcttc ggtcaccagt tctcgcacgt
     3121 ctggatcgac ttccgcggga tccgcgacgt tttcctgcgc gagaagggga tcgactactt
     3181 cgagaactcg cggcgcgccg tcctcgcaca gcgcgcctac gcgatccgga atccggaagg
     3241 gttcctcggc tatggtcccg acgtctgggg cctctcggcc tgcgacggcc cggccgacgt
     3301 gacgcggacg gtcgacggaa aggcccggag gttcgtgacg tacggcgccc gcggagtctc
     3361 ctcgttctgg tcgcatgacg acgggacgat cacgccgatg gccgccgccg cctcgctccc
     3421 gttcgcgccg gaggtcgtcc tccccgcgat ccgggagatg aagcggctcc acggcgagca
     3481 cctctggggc gagtacgggt tcttcgacgc cttcaacccg acgttccggt tcaccgacgt
     3541 tccgaccccg ctcggtaaag tggtgccgga ggccgggtgg tacgcgacga actacctcgg
     3601 gatcgaccag gggccgatcg tcctgatgct cgagaaccag cggagcgggc tcctctggcg
     3661 gctgatgaag cgctcgccgg cgatcgagac cggcctgcgg cgggcggggt tcacgggcgg
     3721 ctggctcgag gaagggacgg cgccggcgcg aagcggggag gcggcgatcc gttgagggcg
     3781 ctcgcctgcg gcctcctcct ggccgcggcg acggcctgcg cgcgcggggg agagcgcggc
     3841 gaggcggtcc gcctctgggc gttcggccgc gagggcgagg tcgtccggga gctcgtcccc
     3901 gagctcgaga agcgccaccc cggcctccgc gtcgaggtgc agcagatccc ctggacggcg
     3961 gcgcacgaga agctcctgac ggcctacgtc gggcgggagc tccccgacgt ggcgcagatg
     4021 gggaacacct ggatcccgga gatgaccgcg ctcgacgcga tcgagccgct cgacgcgcgg
     4081 gtcgccgcga gcgccgccgt ccagccgggc gactacttcc ccgggatctg ggagacgaac
     4141 gtcctcgacg ggacgctcta cggcgtgccg tggtacgtcg acacgcgcct cgtcttctac
     4201 cggacggacc tcctcgccgc cgcaggcgtg cgggatttcc cgaagagctg gagcgcctgg
     4261 cgggccgcga tggaagccgt cgcgaggagg ggcggtccgg gtcgtttcgg gctcctcctc
     4321 ccgacgaacg agtgggcgca gccggtggcc ctcgccgtcg ccgcgggggc ggagctcctc
     4381 tcggacggcg cccgccacgg cgcgttccgg gccccggagt tccggcgggc ggccgagttc
     4441 ttcgtcggcc tctaccgcga cggcctcgcg ccgcctctcg cgaacacgca ggtcgcgaac
     4501 ctctaccagc agatggccgc gggcgacttc gcgatgtacg tcaccgggcc gtggaacctc
     4561 ggcgagttcc ggcgccggct ccccgcgagc ctccagggct cctggacgac cgcgccgctt
     4621 cccgcgcccg acgggatgcc ctggcccggc gtgtcgctcg cggggggctc gagcctcgtc
     4681 gtcttccgtt cctcgagccg gaaggacgac gcctggaagg tcgtcgaatt cctctccgag
     4741 ccggcgacgc agaagcggtt ctggcagctc tgcggcgacc tccccgcgcg gcgctccgcg
     4801 tgggacgagc cggcgctcgc taccgacgcg cggaccgcgc cgttccggct ccagctcgag
     4861 cacgtccggg cgacgccgaa ggtcccggag tgggagcaga tcgccgcaaa ggtctgggag
     4921 cggctggagc cggtggcgcg cgggacgcgc gacctcgacg cggtgctcac cgcgctcgac
     4981 gcggacgtcg acgcgatcct cgcgaagcgc cggtggctcc tcgagaggaa ggccgcggag
     5041 gggaggccgt gaggcgcctc tcctcgtggc gggcgcgcga ggcgcgggcg ggcctcctcc
     5101 tcgtcgctcc cggcctcctc ctcctcgccc tcttcctcgc cctgccggtc gtcgcggcgc
     5161 tcctcctgtc gctgaccgac ttcgacctct acgcgatcgg cgatccgtcg gcggcgcgcg
     5221 tcgtcggcct cgggaactac gcccggctcc tccacgaccc ggtcttctgg aaggcgctcg
     5281 ggaacacgtt cttcttcgtc gtcttcggcg ggccgctcac ggtctccctc tccctcggag
     5341 cggcgctcct cctgaaccac cggctcgtcc gcttcccggg cctcttccgg acgatctatt
     5401 tcgcccccgt cgtcacgacc ctcgtcgcgg tggcggccgt ctggcgctac ctctaccacc
     5461 cccgcttcgg cctcctgaac gccgcgctcg gcttcttcgg gatcggacct gtcgactggc
     5521 tcggcgaccc gcactgggcg atgccggcga tcgtcctgat ggcggcgtgg aagaacttcg
     5581 gcgcgaacat gatcatcttc gtcgccgggc tgaaggcgat cccggagagc ctctacgagg
     5641 cggcgcggat cgacggggcg gggccgatcc agcgcttccg gaagatcacc gtcccgctcc
     5701 tggcgccgac gttcctcttc gtcggcctga cgacgacgat cggctacttc cagctcttcg
     5761 ccgagccgta cgtcctctcg ggcggggagg gggggccgat gaacgcgacc ctctcggtgg
     5821 ccctcttcat gtacaagcag ggcttccgct ggtggagcat cggctcggcc gcgtcgatcg
     5881 ccttcgtcct cttcgccgtg atcctcgccg cgacgctcct ccagagacgg ctccagaagg
     5941 ggggcgaggc gtgagccgga aggcgtggac ctccctcgcc ctccacgcgg cgctcgtcag
     6001 cggggcggtc gtgacgctcg ccccgctcct ctggatgctc tcggcctcgt tcatgacgac
     6061 cggcgaggcg aacgccgcgc cgcctccgct ccttccgggg agggcgacgc tcgcgcagta
     6121 ccgcgagctc ttcgtgaggc tcgacctcgg acgcagcttc gtgaacagct cgatcctcgc
     6181 cctctgcgcg
//
LOCUS       DAOTEZ010000253         6181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_9089_length_6181_cov_7.648057, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000253 DAOTEZ010000000
VERSION     DAOTEZ010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6181
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9089_length_6181_cov_7.648057"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..703)
                     /locus_tag="P5164_13095"
     CDS             complement(161..703)
                     /locus_tag="P5164_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HRY44872.1"
                     /translation="MAGKLAGTKTEANLKEAFAGESMARNKYTYFAEEARKAGLLSIA
                     TVLETTANEEKEHAKRLFKFLDGIAGTDANLEQCIAGENYEWTDMYERMAKEAEEEGF
                     VPIARFFRQVAVVEKHHEERFRRALEDLKAGAVFRKGNEVYWYCTNCGHIEKGLEAPK
                     ACPVCAHPQSYFRAMPEGEV"
     gene            806..1564
                     /locus_tag="P5164_13100"
     CDS             806..1564
                     /locus_tag="P5164_13100"
                     /inference="COORDINATES: protein motif:HMM:NF018963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1460 domain-containing protein"
                     /protein_id="HRY44873.1"
                     /translation="MKTTRSRKTDLRRARSLLALAKPHTSLPARVEALSAALLGAPYV
                     AHALVGSATEAEALVAPLDGFDCVTYVESVLALARSGEPAGYVEELRGLRYDGGRVEW
                     ARRNHFMTDWVRRNARAGVVRAVAPGTLGKRVEKTLSAVPGLPPRRARFACVPKARLR
                     AFLPRLANGDVLLFASTKAGLDVFHCGFLVLAGGSLRLRHASRSAGCVVEEDLVRFLA
                     ANRMAGLVAARPLDPVKTAAPAPARREPARKERS"
     gene            1561..2850
                     /locus_tag="P5164_13105"
     CDS             1561..2850
                     /locus_tag="P5164_13105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY44874.1"
                     /translation="MSLLRPMPVSSEGLALLLADATEEVVFLSDPRVAVTPGPRMFER
                     MAQTLRDTGAGWVYSDSAGHPRIDYRPGSLRDGFDFGPIVAFPLALAREAGVAEGLRW
                     GALYDLRLRLSERAPVVRVPEPLYASGPLETRPSGEAQFDYVDPRNREAQVELERVAT
                     EHLRRIGALLPPEFAPVSLPDERFPVTASVVIPVRNRERTILDAVASALSQQAPFPFN
                     VIVVDNHSTDGTTEMLGGVRDPRLVHVVPQRRDLGIGGCWDEALFHPRCGRYAVQLDS
                     DDLYANEGVLARIVAELEAGPYAMVVASYTMVDFELREIPPGLVDHREWTRENGRNNA
                     LRVNGFGAPRAFDVSILRRIGFPNVSYGEDYAVCLRVSREHEIGRIYDSVYLCRRWEG
                     NSDSALPLETQNRYDAYKDWLRTVELAARMRLLAGRA"
     gene            2856..3749
                     /locus_tag="P5164_13110"
     CDS             2856..3749
                     /locus_tag="P5164_13110"
                     /inference="COORDINATES: protein motif:HMM:NF027594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HRY44875.1"
                     /translation="MRTAVEELFLRQVEEWPLLAAGVAALSGARTRDVGGGGRGPLVR
                     HIPHRAASTTAKVDPASIAKRPCFLCRENLFPEQRGIAFGGDWTLLCNPFPIVERHLT
                     IVHREHRDQRIGGRLGALLDLAAALEGHFVLYNGPRCGASAPDHLHFQAGALEELPVV
                     PVADGAEGPALDAWGARALLLRGARESVVSDAERALALLAGALPQEPEPWVNVVAWRA
                     PDGGFVLLLYPRAKHRPEAFHRGELIVSPAAVDLSGLVVAPVERDFERMAAADLRAVF
                     EEVAIGKEAFGRLLAGIGGSR"
     gene            3746..5878
                     /locus_tag="P5164_13115"
     CDS             3746..5878
                     /locus_tag="P5164_13115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HRY44876.1"
                     /translation="MIVSVGLMEGLPSLEVELLGTFVDPSGARLGPGRVRLDSETTLS
                     PVDPAAAAFAIDGVTIGIGFHWERKERQAFRGTLRLLRRPSGLTVVNDVPLEEYVTSV
                     VSSEMSAACPPELLKAHAVISRSWLKGPSARAVVPPPPSAPGEVLRWYGREAHPDFEV
                     CADDHCQRYQGITKAISPAAAEAVRATAGEMLLFDGEICDARFSKCCGGLTESYATAW
                     DDAEVPYLVSFADGLEGPPPSDLEAFLRAAPAAFCNTADAGLLARILPGFDQETRDFY
                     RWRVAYGAAELGEIVAARLGVDLGPIVALEPLARGPSGRIFRLRISGERGEVVVGKEL
                     EIRRALSRSHLYSSAFVVDRETGPGDEPRFVLRGGGWGHGVGLCQIGAAVMAERGFAY
                     RAILAHYYPGTSVGAVPLPEREIRGPLDLDARSLRLLETHSILNVLNVVVLRIGDVGD
                     LVGDAETADALIARVRAGHPELDEPEKAVAALEKTREVRDAVLAWIAAASEARTGSLR
                     AELDLHAANLASAFDVLAARAAELGRRVPSADPWVRRSLAGLRENLAEAIRAIERNAL
                     GRWRIASGAGDRGPDAYLIEIDLSSRDGETLALPAVLEDVMRDLAANARKYTAPGGAL
                     RVTLAEDGDGLLLRVSDTGRGIPEEEIEEVVRFGRRGRPDGRRQFGGGYGLTKACYVA
                     HAFGGRIWIRSAPGAGTTVTIRIPRPDR"
BASE COUNT          756 a         2346 c         2161 g          918 t
ORIGIN      
        1 gatcggggag gctcgccacg agcctccccg cggcggctgc gccgcctccc ctcggaaggg
       61 gaccgcgaga gtggatcggg gaggctcgcc gcgagcctcc ccgatcctct cggagacgcg
      121 acagagcagg gccttcaggc ccggccccgt cggcggtgtc tcagacctcg ccttccggca
      181 tcgcgcggaa gtacgactgc gggtgggcgc agacggggca cgccttcggc gcctcgagcc
      241 ccttctcgat gtgcccgcag ttcgtgcaat accagtagac ctcgttcccc ttcctgaaga
      301 cggcgcccgc cttcaggtcc tcgagcgccc tgcggaaccg ctcctcgtga tgcttctcga
      361 cgacggcgac ctgccggaag aagcgggcga tcgggacgaa tccctcttcc tcggcctcct
      421 tcgccatccg ctcgtacatg tcggtccact cgtagttctc gccggcgatg cactgctcga
      481 ggttcgcgtc ggtcccggcg atgccgtcga ggaacttgaa gaggcgcttg gcgtgctcct
      541 tctcctcgtt cgccgtcgtc tcgaggacgg tcgcgatgct caggagcccc gccttccggg
      601 cctcctccgc gaagtaggtg tacttgttcc gggccatcga ctcgcctgcg aaggcttcct
      661 tcaggttcgc ctcggtcttc gtccctgcca gctttccggc catcgtgctc tccttcgggt
      721 ccggcggctt cggggcgccc acggccctgc cgccgggcca acggacgagg ttaccgcagc
      781 gggtcgcggg taacatcgcc cgccgatgaa gacgacccgt tcccggaaga ccgaccttcg
      841 gcgggcccgc tccctcctcg ccctcgcgaa gccccacacg agcctcccgg cgcgcgtcga
      901 ggcgctctcg gcggcgctcc tcggcgctcc ctacgtcgcc cacgccctcg tcggctcggc
      961 gacggaagcg gaggcgctcg tcgcgccgct cgacggcttc gactgtgtca cgtacgtcga
     1021 gagcgtcctc gcgctcgcac gctcggggga gccggccggg tacgtcgagg agctccgcgg
     1081 gctccgctac gacggcggcc gcgtggagtg ggcgaggcgg aaccacttca tgaccgattg
     1141 ggtccggcga aacgcgcgtg ccggcgtggt gcgcgcggtc gcgccgggta cgctcggaaa
     1201 acgggtcgag aagacgctct ccgccgtccc cgggctcccg cctcggcggg cccgcttcgc
     1261 ctgcgtcccg aaggcccgcc tgcgcgcctt cctcccgcgc ctcgcgaacg gcgacgttct
     1321 cctcttcgcc tcgacgaagg cggggctcga cgtcttccac tgcggcttcc tcgtcctcgc
     1381 aggcggctcg ctccgccttc gccacgcctc ccggagcgcc ggttgcgtcg tcgaggagga
     1441 cctcgtgcgc ttcctcgccg cgaaccggat ggcggggctg gtcgcggcgc ggccgctcga
     1501 tccggtgaag accgccgcgc ccgcccccgc gcggcgcgag ccggcccgga aggagcgctc
     1561 gtgagcctcc tccgcccgat gccggtctcc tcggaggggc tcgcgctcct cctcgccgac
     1621 gcgacggagg aggtcgtctt cctctccgac ccgcgcgtcg ccgtgacgcc cggcccgcgg
     1681 atgttcgagc ggatggcgca gacgctccgc gacacggggg cgggctgggt ctactccgac
     1741 tctgccggcc acccgcggat cgactaccgc cccggcagcc tccgcgacgg cttcgacttc
     1801 gggccgatcg tcgcgttccc gctcgccctc gcgcgggagg ccggagtcgc ggagggcctc
     1861 cgctggggag cgctctacga ccttcgcctc cgcctctccg agcgcgcccc cgtcgtccgc
     1921 gtcccggagc cgctctacgc ctccgggccg ctcgagacgc gcccctccgg cgaggcgcag
     1981 ttcgactacg tcgacccgag gaaccgcgag gcccaggtcg agttggagcg cgtcgccacg
     2041 gagcacctcc ggcggatcgg tgccctcctc ccgcccgagt tcgcgcccgt ctcgctcccc
     2101 gacgagcgtt tcccggtgac ggcgagcgtc gtcatccccg tgaggaaccg ggagcggacg
     2161 atcctcgacg ccgtcgcgag cgccctctcg cagcaggcgc cgttcccgtt caacgtgatc
     2221 gtcgtcgaca accactcgac cgacgggacg accgagatgc tcggcggcgt gagggacccg
     2281 cgcctcgtcc acgtcgtccc ccagcggcgc gacctcggga tcggcggctg ctgggacgag
     2341 gcgctcttcc acccgcgctg cggccgctac gccgtccagc tcgactccga cgacctctac
     2401 gcgaacgagg gcgtcctcgc ccggatcgtc gccgagctcg aggcggggcc gtacgcgatg
     2461 gtcgtcgcct cgtacacgat ggtcgacttc gagctgaggg agatcccgcc cggcctcgtc
     2521 gaccaccggg agtggacgcg ggagaacggc cggaacaacg ccctccgcgt gaacggcttc
     2581 ggtgcgccgc gcgccttcga cgtctcgatc ctccggagga tcgggttccc gaacgtgagc
     2641 tacggcgagg actatgccgt ctgcctgagg gtcagccgcg agcacgagat cgggcggatc
     2701 tacgactccg tctacctctg ccggcgctgg gaggggaact ccgacagcgc cctcccgctc
     2761 gagacgcaga accgctacga cgcctataag gactggctcc gaacggtcga gctcgccgcc
     2821 cgaatgcggc tcctcgcggg gcgcgcctga ccgcgatgcg cacggcggtc gaggagctct
     2881 tcctccggca ggtcgaggag tggccgctcc tcgccgccgg cgtggcggcg ctctccgggg
     2941 cgcggacgcg ggacgtcggc ggaggggggc gcggcccgct cgttcgccac atcccgcacc
     3001 gcgcggcgag cacgacggcg aaggtcgacc ccgcctcgat cgcgaagcgc ccctgcttcc
     3061 tctgccgcga gaacctcttc cccgagcagc ggggaatcgc cttcggaggc gactggacgc
     3121 tcctctgcaa cccgttcccg atcgtggaga ggcacctgac gatcgtccac cgcgagcacc
     3181 gcgaccagcg gatcggaggt cgcctcggcg ccctcctcga cctcgccgcc gcgctcgaag
     3241 ggcacttcgt cctctacaac ggcccccggt gcggcgcctc tgcccccgac cacctccact
     3301 tccaggcggg cgcgctcgag gagctgccgg tcgttcccgt cgcggacggt gcggaaggcc
     3361 cggccctcga cgcctggggt gcgcgggcgc tcctcctccg gggcgcccgc gaatccgtcg
     3421 tctccgacgc ggagcgcgcc ctcgcccttc tcgccggcgc gctcccgcag gagccggagc
     3481 cgtgggtcaa cgtcgtcgcc tggcgggcgc cggatggcgg cttcgtcctt ctcctctacc
     3541 cgcgggccaa gcaccgccct gaggcgttcc accgcggcga gctgatcgtc agcccggcgg
     3601 cggtcgacct ttccggcctc gtcgtcgcgc cggtggagag ggacttcgag cggatggcgg
     3661 ccgccgacct ccgggcggtc ttcgaggaag tcgcgatcgg caaggaggcc ttcggccgcc
     3721 tcctcgccgg gatcggaggt tcgcggtgat cgtctccgtc ggcctgatgg aagggctccc
     3781 gtccctcgag gtcgagctcc tcgggacttt cgtcgatccg tccggtgcgc ggctcggacc
     3841 gggacgcgtt cggctcgact ccgagacgac gctctcgccg gtggacccgg ccgccgcggc
     3901 gttcgcgatc gacggcgtga cgatcggcat cgggttccac tgggagcgga aggaacgcca
     3961 ggcctttcgc ggaacgctcc gtctcctccg gcgaccctcc gggctgacgg tcgtcaacga
     4021 cgtcccgctc gaggagtacg tcacgagcgt cgtctcctcc gagatgagcg ccgcctgccc
     4081 gccggagctc ctgaaggcgc acgccgtcat ctcgcgcagc tggctgaagg gcccctcggc
     4141 gcgcgccgtc gtcccgccgc ccccctcggc gccgggcgag gtcctccgct ggtacggacg
     4201 ggaggcgcac cccgacttcg aggtctgcgc cgacgaccac tgccagcgct accaggggat
     4261 cacgaaggcg atctctccgg cggcggccga ggcggtccgc gcgacggcgg gagagatgct
     4321 cctgttcgac ggcgagatct gcgacgcccg cttctcgaaa tgctgcggcg gcctcaccga
     4381 gtcgtacgcg acggcgtggg acgacgcgga ggtcccctac ctcgtctcct tcgcggacgg
     4441 cctcgagggg ccgcccccct ccgacctcga ggcgttcctt cgcgccgcgc cggcggcgtt
     4501 ctgcaacacc gcggacgccg ggctcctcgc gcggatcctt cccggcttcg accaggagac
     4561 gcgcgacttc taccgctgga gggtcgcgta cggcgccgcc gagctgggcg agatcgtcgc
     4621 cgcgcgcctc ggcgtcgacc tcggcccgat cgtcgcgctc gagccgctcg cccgcggccc
     4681 ctcgggccgc atcttccgcc tccggatctc gggggagcgc ggcgaggtcg tcgtcggcaa
     4741 ggagctcgag atccgtcgcg ccctctcgcg ctcgcacctc tacagctcgg ccttcgtcgt
     4801 cgatcgcgag acgggccccg gcgacgagcc gcgcttcgtc cttcgcgggg ggggctgggg
     4861 gcacggcgtc ggcctctgcc agatcggggc ggccgtcatg gccgagcgtg gcttcgccta
     4921 ccgggcaatc ctcgcgcact actacccggg gacgtcggtc ggcgccgtgc cgctgccgga
     4981 gcgggagatc aggggaccgc ttgacctcga cgcgcgctcc ctgcggctcc tcgagaccca
     5041 ctcgatcctg aacgtcctga acgtggtcgt cctccggatc ggcgacgtcg gcgacctcgt
     5101 gggcgacgcg gagacggcgg acgcgctgat cgcgcgcgtc cgcgccgggc acccggagct
     5161 cgacgagccc gagaaggcgg tcgcggcgct cgagaagacc cgcgaggtcc gcgacgccgt
     5221 cctcgcctgg atcgccgcgg cgagcgaggc gcgcaccggc tccctccggg cggagctcga
     5281 cctccacgcg gcgaacctcg cctccgcctt cgacgtcctc gcggcccgcg ccgcggagct
     5341 gggccgtcgc gtgccgagcg cggacccgtg ggtccgtcgc tcgctcgccg ggcttcgcga
     5401 gaacctcgcg gaagcgatcc gtgcgatcga gcggaacgcg ctcggccggt ggcggatcgc
     5461 ctcgggagcg ggagaccgcg gccccgacgc ctacctgatc gagatcgacc tctcgagccg
     5521 cgacggagag accctcgccc tgccggccgt cctcgaggac gtcatgcgcg acctcgccgc
     5581 gaacgcgcgg aagtacacgg cgccgggagg cgcgctccgc gtgacgctcg cggaagacgg
     5641 cgacgggctg ctcctccggg tcagcgacac ggggcggggc attcccgagg aggagatcga
     5701 ggaggtcgtg cgcttcggac ggcgcggacg tcccgacggc cgaaggcagt tcggcggcgg
     5761 ctacggcctg acgaaggcct gctacgtcgc ccacgcgttc gggggacgga tctggatccg
     5821 ctccgctccg ggcgcgggga cgacggtcac gatccggatc ccgcgccccg atcgctgaca
     5881 cgggacgctc cgaagactcg gaacgggctc gctccgggcc ggcgggggcg agctgttgcc
     5941 gttctcatcg ccccgcaacg gccgggcgag agccccggaa tgggccttca actcattcat
     6001 tacctgtcat ttgcgtcgtc gcagcggcgg gtgcggtgtt gagaaggtca acgggggcat
     6061 ccggggcgtc ctcgcgctcc tcgaacgtaa gcgtctgcgc tggaaatgct tgcgccgatc
     6121 tgccggggcg tggcagcgcc cttgcgatat ctccgggcga agcgggccga gagaaatcgg
     6181 g
//
LOCUS       DAOTEZ010000254         6179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_9096_length_6179_cov_5.983508, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000254 DAOTEZ010000000
VERSION     DAOTEZ010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6179
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9096_length_6179_cov_5.983508"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="P5164_13120"
     CDS             <1..446
                     /locus_tag="P5164_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44877.1"
                     /translation="IASSRTYTEEPGKSYGLAIGLLGDSEAEPGDEAALTFLSGGPRT
                     RTNLGFLETRGLETVAEVTLFDASGSILATRELMLEPHQAVQWNDVFAEMGIAPVEEA
                     SATVSVGRGGALLVHATRVDNATNDGSFLPARVVRSRPVTPAFAR"
     gene            complement(424..1098)
                     /locus_tag="P5164_13125"
     CDS             complement(424..1098)
                     /locus_tag="P5164_13125"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY44878.1"
                     /translation="MSGPSTPTLPGPRAVFRAASALPLGARLHLAVRRWSAPWPRVVD
                     GLEGERTLLDVGCGPGLLAFLLESRGAAGSYVGIDPDERKVSRARAWLGRLPRRTFRA
                     CELSEIRKETFDAVSVVDVLYLVPRAEREAFVAAVAARLDPGGRVVVLTSGGGPRWKR
                     LLDRLQERLVVAVLGWTRGSAVEPCDGAEVAALLAAAGLVDVRVEDAGAGHLHGFELV
                     SGRKPE"
     gene            1144..2304
                     /locus_tag="P5164_13130"
     CDS             1144..2304
                     /locus_tag="P5164_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44879.1"
                     /translation="MSHPTFPWPWRGPENRKLALVVLLWLAALQLWAFVSVRALPIGR
                     PSPWSEEATRRAPAFARFDSGWYNSIVEKGYPPPPAAGAQSEHAFFPLYPMTARAIHL
                     ATGLDSFRAAVVVSWTALLLAVPLLAAEARERFGEGTDRGALPFLLLYPVAFFLAAVY
                     TESLFLLLALLAFRASRRNELAPALLFGLLLGLTRAPAVAVGPGLAVAWWLAGAAGAR
                     RAAGAALLLVAPLAGVLAWVFGIGLLQGEPMLFFRSMSAWRASAGNPFAGAVAFVLEP
                     VRMLRTGWFREHPGAVAPFVHFGLFALLGAFQLRMRRWADAAWTAGVLGLAVATGTAD
                     GVPRYTLTVYPGHFAAAALCETRPRLKALWLGTSLALLLLNSAFFVSWHFVS"
     gene            2307..3035
                     /locus_tag="P5164_13135"
     CDS             2307..3035
                     /locus_tag="P5164_13135"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY44880.1"
                     /translation="MRPHRSVSLVVPMRNEERSLPHLLRVAVEALERHADEWEIVLVD
                     DASSDGTPAFAEEAAGADPRVRVVRHAVNRGLGGALKSGFSAAAKEWVLYSDCDLPWD
                     LEETGRVFRAAELTGADVVSGYRHDRTGEGTLRTIYSFLYNGLVHLAFGFPIRDVNFS
                     CKLVRRSLLSGLPLESEGSFIDAELLVRLRNRGAVIQQVGLDYFPRTRGVSTLSSPRT
                     ILRILRDLARLVPELRGERRRLAP"
     gene            3032..3886
                     /locus_tag="P5164_13140"
     CDS             3032..3886
                     /locus_tag="P5164_13140"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HRY44881.1"
                     /translation="MTRLVLNADDAGLAPGLSRRIASLLASGRLGGTSLLACGEAFGE
                     AVRALREAGVGEAGVHLCLVGGEAPLSPPGRVRGIAPGGRFPGSWPAVAARIASGLAP
                     VAEVEREWEAQVVAVLSAGLRVTHLDAHQHLHLLPRLVPVAAALARRFDVPYVRAPRG
                     DDPAATADPGLAGSVRARLLALFGSSARRLLSRAGLPEPPRVLGLAEAGGMDLARWRT
                     LLDALPSEGTFEVVLHPGVEDARARMRYPWGYSWEAETAALQDDGLLETMSARGVARV
                     GFGELARA"
     gene            complement(3900..4538)
                     /locus_tag="P5164_13145"
     CDS             complement(3900..4538)
                     /locus_tag="P5164_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44882.1"
                     /translation="MTRASRWIALIVSTTLAGSAVAAEKASPVTRLGKTVVRYQDDAV
                     KAVLSWRFANQTFEKEPWLLLELAFAAEGKALNLNREDVALLGPDGERIPLPGQKRFA
                     ERMKNPLQLVQRASVARDPLDNYFSRPTRFESLRFFTVPGGPVVQDEVSGGPSWLIQG
                     DLFFEAPSGIWKPGSYTLVLKNKTLDVALPFSLPAGELRKDAKGADGKVVPW"
     gene            4720..5220
                     /locus_tag="P5164_13150"
     CDS             4720..5220
                     /locus_tag="P5164_13150"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HRY44883.1"
                     /translation="MKNGTSRKGLAAVAAVLGLLLAAGAATAGPAGRRGGASGDLRGA
                     LEALDLSDGQRAELKAVLEEGRERREALRRESWDARQALRTAAEAPAPDAAEVGRAYL
                     RLRESRRSNVEAREKTKERIASILTVEQKAKLEELRSRRGAQRDERPGRPGRDRGLRG
                     PRPPVR"
     gene            complement(5266..>6179)
                     /locus_tag="P5164_13155"
     CDS             complement(5266..>6179)
                     /locus_tag="P5164_13155"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRY44884.1"
                     /translation="RRLTYKYRLVGFDDGWSVTRRRFADYTSLPPGRYDFEVVAIGDA
                     GLASPGATTLEVRVLPRFTQTVAFRVSVVLAALLFAYALHRQRIRRMAAQEQQLNRVV
                     AEKTEALAEANARLEELSLSDALTGIANRRHFDRRLEEEWRRCRRFGLPLAEILLDVD
                     LFKAYNDALGHPAGDECLRRVAGALSDRLRRAGDLVARYGGEEFVALLPGLSLADAYV
                     VAEHLRTRVEALRIPHPSAKDAIVTVSAGVASVVPGHGPASRLTSAADAALYEAKRRG
                     RNQSVAAGRERPEGVAVDRPDTPPGSG"
BASE COUNT          757 a         2350 c         2191 g          881 t
ORIGIN      
        1 tgatcgcttc cagccgcacc tacaccgagg agccggggaa gagctacggc ctcgccatcg
       61 gcctcctcgg cgactccgaa gcggagcccg gagacgaggc ggccctgacg ttcctctcgg
      121 gggggccccg gacgcggacg aacctggggt tcctcgagac gcgcggtctc gagacggtgg
      181 cggaggtgac gctcttcgac gcgtcgggct ccatcctcgc cacccgcgag ctgatgctcg
      241 agccccatca ggccgtccag tggaacgacg tcttcgcgga gatgggcatc gccccggtcg
      301 aggaagcctc cgcgacggtg agcgtgggcc ggggcggggc gctcctcgtc cacgcgaccc
      361 gcgtcgacaa cgcgacgaac gacgggagct tcctccctgc ccgcgtcgtc cgctcccgcc
      421 ccgtcactcc ggctttcgcc cgctgaccag ctcgaatccg tgcagatggc cggcgccggc
      481 gtcctcgacg cggacgtcga cgagtccggc ggccgcgagg agcgccgcca cttcggcgcc
      541 gtcgcacggc tcgacggcgg agccccgcgt ccagccgagg accgccacga cgagccgctc
      601 ctggagccgg tccaggagcc tcttccaccg ggggccgccg ccgctcgtca ggacgacgac
      661 gcgcccgccg gggtcgagac gcgccgcgac ggcggcgacg aaagcctcgc gttcggcccg
      721 gggcaccagg tagaggacgt cgacgaccga gaccgcgtcg aatgtctcct tccggatctc
      781 cgagagctcg cacgcgcgga acgtccgccg cggcaggcgg ccgagccagg ctcgcgcccg
      841 cgacaccttc ctttcgtcgg ggtcgatgcc gacgtaggag ccggcggccc cccgagactc
      901 gaggaggaac gccaggaggc cgggcccgca cccgacgtcg aggagcgtcc tctcgccctc
      961 gaggccgtcc acgacgcgcg gccagggcgc gctccacctc cgaacggcga ggtggagccg
     1021 cgcgccgagc gggagcgcgg acgccgcgcg gaagacggcc cgcgggccgg ggagcgtggg
     1081 cgtcgacggg ccggacacgg gccatagagt aatctccgat cctgcggtgc gaggcctccc
     1141 gagatgagcc atccgacctt tccctggccg tggcgcggcc cggagaaccg caagctcgcg
     1201 ctcgtcgtcc tcctgtggct cgccgcgctc cagctctggg cgttcgtctc ggtccgcgcg
     1261 cttccgatcg gccggccttc tccgtggagc gaggaggcga cgcggcgggc cccggccttc
     1321 gcccgcttcg actcgggctg gtacaactcg atcgtcgaga agggctatcc gccgccgcct
     1381 gccgcgggag cccagtcgga gcacgccttc tttccccttt atccgatgac cgcgcgcgcg
     1441 atccacctcg cgacgggtct cgactcgttc cgcgccgccg tcgtcgtcag ctggaccgcg
     1501 ctcctcctcg ccgtccccct cctggccgcc gaggcgcgcg aacgcttcgg cgaggggacc
     1561 gaccggggcg cgctcccgtt cctgctcctc taccccgtcg ccttcttcct cgccgcggtc
     1621 tacaccgagt cgctcttcct cctcctcgcg ctcctcgcgt tccgcgcctc ccgacggaac
     1681 gagctcgcgc ccgcgctcct cttcggcctc ctcctcggcc tcacccgcgc ccccgccgtc
     1741 gcggtcgggc cgggcctggc ggtcgcgtgg tggctggccg gcgcggccgg cgcgcgccgc
     1801 gcggcgggag ccgcgctcct tctcgtcgca cctctcgccg gagtcctcgc ctgggtcttc
     1861 gggatcggcc tcctgcaggg agagccgatg ctcttcttcc gttcgatgag cgcctggcgc
     1921 gcctcggcgg gcaacccctt tgccggcgcc gtcgccttcg tcctcgagcc ggtccggatg
     1981 ctccgcaccg gctggttccg ggagcacccg ggcgccgtcg ccccgttcgt ccacttcggc
     2041 ctcttcgcgc tcctgggagc gttccagctc cggatgcgcc gatgggcgga cgccgcctgg
     2101 acggcagggg tcctcggcct cgccgtggcg accgggacgg ccgacggcgt cccccgctac
     2161 acactcaccg tctacccggg ccacttcgcc gccgcagccc tctgcgagac ccggccccgt
     2221 ctgaaggcgc tctggctcgg aacgtcgctc gcgctcctcc tcctgaactc cgcgttcttc
     2281 gtgagctggc acttcgtttc gtagcgatgc ggcctcaccg atcggtctcc ctcgtcgtcc
     2341 cgatgcgcaa cgaggagcgg agcctccccc acctcctgcg cgtcgcggtc gaagcgctcg
     2401 aacggcacgc ggacgagtgg gagatcgtcc tcgtcgacga cgcctcgtcc gacgggaccc
     2461 ccgcgttcgc cgaggaggcc gccggcgccg atccgcgcgt tcgggtcgtt cggcacgccg
     2521 tgaacagggg gctgggcggc gcgctgaagt ccggcttctc ggccgcggcg aaggagtggg
     2581 tcctctactc cgactgcgat ctcccgtggg acctcgagga gacggggcgc gtcttccgcg
     2641 ccgccgagct gacgggcgcc gacgtcgtct ccggctatcg ccacgaccgg accggcgagg
     2701 ggacgctccg aacgatctac tcgttcctct acaacgggct cgtccacctc gcgttcggct
     2761 tcccgatccg cgacgtcaac ttctcctgca agctcgtccg gcgctcgctc ctctcggggc
     2821 tccccctcga gagcgagggc tccttcatcg acgccgagct cctcgtccgg ctccggaacc
     2881 ggggcgcggt catccagcag gtcggcctcg actacttccc gcggacgcgc ggcgtctcca
     2941 cgctctcctc gccgcggacg atcctccgca tccttcgcga cctcgcgcgt ctcgtcccgg
     3001 agctccgggg cgagcgccgc cggctcgcgc cgtgacgcgg ctcgtcctga acgccgacga
     3061 cgccggcctc gcgcccggcc tctcgcggcg gatcgcttcg ctcctggcgt cggggcgcct
     3121 cggggggacg agcctcctcg cctgcggaga ggcgttcggc gaggcggtcc gcgctctccg
     3181 agaggccggg gtcggagaag ccggcgtcca tctctgcctc gtcgggggcg aggcgccgct
     3241 ctctcccccg gggcgcgtgc gcgggatcgc gccgggaggg cgcttcccgg gttcgtggcc
     3301 ggccgtcgcg gcgcggatcg cgtccggcct cgccccggtc gcggaggtgg agcgggaatg
     3361 ggaggcccag gtcgtcgccg tcctctcggc gggcctgcgg gtgacgcatc tcgacgcgca
     3421 ccagcacctc cacctcctcc cgcgcctcgt tccggtggcc gccgcgctcg cgcggcgctt
     3481 cgacgtcccg tacgtccggg cgcctcgagg ggacgacccg gccgcgacgg ccgatcccgg
     3541 cctcgcggga agcgtccggg cgcggctcct cgcgctcttc gggtcgagtg cgagacggct
     3601 cctctcgcgg gccggcctgc ccgagcctcc gcgcgtcctc ggcctcgcgg aggcgggagg
     3661 aatggacctc gcgcggtggc ggacgctcct cgacgcgctg ccgtccgagg ggacgttcga
     3721 ggtcgtcctc catcccgggg tcgaggacgc ccgggcgcgc atgcgctacc cgtggggcta
     3781 ttcgtgggaa gcggagaccg ccgccctcca ggacgacggc ctcctcgaga cgatgtcggc
     3841 gcggggcgtg gcgcgcgtcg gcttcggaga gctcgcgcgc gcctgacgcc ccgtccgcgt
     3901 caccagggga cgaccttgcc gtcggccccc ttcgcgtcct tccgcagctc gccggccggc
     3961 agagagaacg gcagcgcgac gtccagggtc ttgttcttca ggacgagggt gtagctcccc
     4021 ggcttccaga tgccgctcgg cgcctcgaag aagaggtccc cctggatcag ccagctcggg
     4081 ccgccggaga cctcgtcctg cacgaccgga ccgcccggca ccgtgaagaa gcgaaggctc
     4141 tcgaaccgag tcggcctcga gaagtagttg tccagcgggt cccgcgcaac cgaagcccgc
     4201 tgcacgagct ggagagggtt cttcatcctt tccgcgaagc gcttctggcc cgggagcggg
     4261 attcgctctc cgtccggccc cagcagcgcg acgtcctcgc ggttcaggtt cagggccttc
     4321 ccctccgcgg cgaacgcgag ctcgaggagg agccaaggct ccttctcgaa cgtctgattg
     4381 gcgaatcgcc acgagaggac ggccttgacg gcgtcgtcct ggtagcggac gacggtcttc
     4441 ccgaggcgcg tgacgggcga cgccttctcc gcggcgacgg cggagccggc gagcgtcgtg
     4501 gagacgatca gggcgatcca acggctggcg cgggtcatcg gaacctcctc accgtccttg
     4561 cgcgaggtcg atcctgcgcg tcgggacgga ctgagacgac gtctagcaca tcccgcgcca
     4621 gcgcgtcggg ggacgacgtc aacgggatgc gcgtcgagtg cgtctcatgg ttcgtccggc
     4681 ccgggcgatc gtcggccccc ggacgcggaa ggagcagcca tgaaaaacgg aacgtcacgc
     4741 aaggggctcg ccgcggtcgc cgcggtcctg ggactcctcc ttgccgccgg tgcggcgacg
     4801 gccgggccgg cgggacgccg gggcggcgca tcgggcgacc tccggggagc cctggaggcc
     4861 ctcgacctct ccgacgggca gcgcgccgag ctcaaggcgg tcctcgagga agggcgcgag
     4921 cgccgcgagg ccctccggcg cgagagctgg gacgcccgcc aggctctccg cacggcggcg
     4981 gaggcgcccg ccccggacgc ggccgaggtg ggacgcgcct atctccggct tcgggagagc
     5041 cgccggtcga acgtcgaggc ccgcgagaag acgaaggaga ggatcgcgtc gatcctgacc
     5101 gtcgagcaga aggcgaagct cgaagagctg cgttcccggc gcggcgcgca gcgcgacgag
     5161 cgcccgggcc gtcccggaag ggatcgcggg ctccgcggtc ctcggcctcc tgtccgttga
     5221 cccgactcga ccccgtcgtc cggccgagct cccgccccgc ccggttcagc cgcttccggg
     5281 cggggtgtcc ggccgatcca cggcgacccc ctcgggccgc tcccttcccg ccgccacgga
     5341 ttggtttcgt cccctccgct tcgcctcgta gagggcggcg tccgcggcgg aggtcagccg
     5401 agacgccggg ccgtgtcccg gaacgacgga tgcgaccccg gcgctgacgg tcacgatggc
     5461 gtccttggcg gacggatggg ggatccggag cgcctccacc ctcgtccgga gatgctcggc
     5521 gacgacgtag gcgtccgcga gagacaggcc cggcaggagc gcgacgaact cctcgccacc
     5581 atatcgcgcg acgaggtccc cggcccgtcg aagccggtcg gagagcgcgc cggcgacccg
     5641 acgcaggcat tcgtcgccgg ccggatgacc gagggcgtcg ttgtaggcct tgaacaggtc
     5701 gacgtcgagg aggatctccg cgagcggaag gccgaacctc cggcagcgcc gccactcctc
     5761 ctcgagccgg cggtcgaagt gacgccggtt cgcgattccc gtgagcgcgt cggaaagcga
     5821 cagctcctcg agacgggcgt tcgcctcggc cagcgcctcc gtcttctcgg cgacgacccg
     5881 gttcagctgc tgctcctgcg ccgccatccg gcggatgcgc tgccggtgga gggcgtaggc
     5941 gaagaggagc gccgcgagga cgacggagac ccggaacgcg acggtctggg tgaagcgcgg
     6001 caggacccgc acctcgagcg tcgtcgcccc ggggctcgcg agtccggcgt cccctatcgc
     6061 caccacctcg aagtcgtacc tcccgggagg gagcgacgtg tagtccgcga accggcgacg
     6121 cgtgacgctc cacccgtcgt cgaagccgac gaggcggtac ttgtaggtaa ggcggcggc
//
LOCUS       DAOTEZ010000255         6160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_9127_length_6160_cov_5.692875, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000255 DAOTEZ010000000
VERSION     DAOTEZ010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6160
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9127_length_6160_cov_5.692875"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2397
                     /locus_tag="P5164_13160"
     CDS             <1..2397
                     /locus_tag="P5164_13160"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRY44885.1"
                     /translation="QVQELAGLGSYRLDARTGVWTSSAVLDRIFGIGPDFVRDVEGWL
                     ALVHPGDREEMRAYFRDEVLGRGRRFEKDYRVVRRSDGEVRWVHGVGDLERDERGAVV
                     RMLGAILDITDRHRASEEVRESRELLSRFIRHSPIYAFVKDVSASESRVLFASENYEG
                     MIGIPGSRMAGKTMEELFPPEFAAKITADDQAVVARGEVLQLDEELGGRSYTTIKFPI
                     PLGERRLLAGYTIDIQERKEAEKRRLEAERRFRLLAEATSEGVVLIDEGRILECNARF
                     AAMHGFEPAALLGLPFLALVAPESLEHVSARHVSETTGRHRWIALRADGSTFPVEGDA
                     RLVPWEGRTVRIKACRDLTEELRAAAEVARVARLYAALIEINEAIVRVRTPEELFAEV
                     PRILVETGGFRMAWIGLKREDSPRVDVASRFGDETGYLAGIEVRVDSRPEGLGPTGRA
                     IREGRTQVCNDFLNDPSTLPWRDRAKQASWRASAALPIRRRGLPVGALTVYALEVGYF
                     GERERALLEESAADVSYALEHLDQAAETRRAEAEVRELNSELERRVEERTAQLEAAIR
                     ELEAFSYSVSHDLRAPLRAIEGFAGMIVEDAAGALPEETLRRLDVIRTNARRMSALID
                     ALLRFSRLGRHELQRDRVDMAGLARSAFEEAVSDGAARARIELRIGELPDAEGDASLL
                     RQVWVNLLGNAAKYSAGRERPVVEVTAVPGDAETTYRVRDNGVGFDPRYVEKLFRVFE
                     RLHAPDAFEGTGIGLALVKRIVQRHGGEVSAEGREGEGACFSFSVPSASGKARKRRTK
                     "
     gene            complement(2450..3205)
                     /locus_tag="P5164_13165"
     CDS             complement(2450..3205)
                     /locus_tag="P5164_13165"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRY44886.1"
                     /translation="MDGSPFDRWLEDLERRHLAELSFAQVRKGLQAVSSLYVERRGRI
                     GAGAVFDGEGKRAAFAIFYGPLHHLVVGRIVAALGAAEPGLSRVLDIGCGTGAAGAAW
                     ALAAGAEVFGVEKSGWAASEARRTYAALSLRGGATAGDLLRERLPGRGAGILLGWVVN
                     ELETTSRAALLPRLLEAARRGARVLVVEPIAARPVPWWTEWAAAFVADGGRDDLWRLP
                     ATLPSVVARLDRAAGLDHRILTARSLYLPGRAA"
     gene            complement(3228..4454)
                     /locus_tag="P5164_13170"
     CDS             complement(3228..4454)
                     /locus_tag="P5164_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44887.1"
                     /translation="MSVHATRSRLASAAALALLAAPLAPAPADACTTAVVAPSASRSG
                     RPMLWKNRDADGSDNQVVFLADGRYPYVGVVNRGDAAGLQVWAGINSAGFGVMNSASY
                     NLESGDTAAEGTFMKLALQSCATVDEFEALLVRSNAGGRDVSANFGVIDAKGGAAVFE
                     TSAKGYVRVDAAPSSDGGRGLLVRSNYSRSGAKESGTGFLREARAIELLGPAAAGGLD
                     LAAILRAARDVANAGIGQTGEEAGPALVYTGDSVCRLDTVSAIVLEAPAPGEGPEAAA
                     LWIVPSLPLAGAAVPVFPLAGVVPAALAADRGPAPLAASALELRKRLYPDARGEKKRY
                     MDRRVLAAVKARALPGLLAAEEATRAEVEAARGAWRRSRPAPADAAKLSASAAERALA
                     AGEAAVAAVAKELGAP"
     gene            4584..5792
                     /locus_tag="P5164_13175"
     CDS             4584..5792
                     /locus_tag="P5164_13175"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRY44888.1"
                     /translation="MQDRPRRDTFLPFSRPMIGEEEIAGVVDTLRSGWITTGPRAARF
                     EEELAAFNEVPRCLALLSATTGLELSLSALGIGEGDEVITTAHTFVCTVSNVVLRGAV
                     PVLVDIEPDTMNLDAERIEERITPRTKAILPVHYGGLPCRMERIWEISRRHGLAVVED
                     AAHTMGARYAGRRIGSDPRSAFSVYSFHPNKNLTTIEGGAIAFHDLRHEAHLKLQRFH
                     GIARGAGTPYPEGSPLYDVPTPGRKSNFTDVQAAVGLAQLPKLDGFAARRRELARLYL
                     SLLSEVPEVELPADGDAEREHCWNLFVLRNRPEATGLSRDELTAALKAENVGTGIHWQ
                     GLFSFSFYRDYFERHPEGLPKDGLPHAARTTENLMSLPLFPALTEEDVLDVVSAVRRV
                     VAAGRRRARG"
     gene            complement(5847..>6160)
                     /locus_tag="P5164_13180"
     CDS             complement(5847..>6160)
                     /locus_tag="P5164_13180"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY44889.1"
                     /translation="YPFPGNVRELKHAVEQAAVFGDGPVLGPTDFRALWGRSQMLLPA
                     TEPRGGRTPIVDVTPTRLEEALRKTGGNRLEAARLLGISRSSFYRILRRLPAAEASSS
                     D"
BASE COUNT          835 a         2287 c         2258 g          780 t
ORIGIN      
        1 caggtccagg agctcgccgg cctcggaagc tatcgcctcg acgcccggac gggcgtctgg
       61 acgagctcgg cggtcctcga tcgcatcttc gggataggtc ccgacttcgt ccgcgacgtc
      121 gaagggtggc tggccctcgt ccaccccggg gaccgcgagg agatgcgcgc ctacttccgc
      181 gacgaggtcc tcgggcgggg gcggcgcttc gagaaggact accgcgtcgt ccggcgctcc
      241 gacggcgagg tccgctgggt ccacggcgtc ggcgacctcg agcgcgacga aaggggcgcc
      301 gtcgtccgga tgctcggcgc gatcctggac atcaccgacc ggcaccgcgc gagcgaggag
      361 gtccgcgaga gccgcgagct cctctcccgg ttcatccgcc actcgccgat ctacgcgttc
      421 gtgaaggacg tctcggcgtc ggagagccgg gtcctgttcg cgagcgagaa ctacgagggg
      481 atgatcggca tccccggctc gcggatggcc gggaagacga tggaggagct cttcccgccc
      541 gagttcgccg cgaagatcac ggccgacgac caggccgtcg tcgcccgggg cgaggtcctc
      601 cagctcgacg aggagctcgg cggccggagc tacacgacga tcaagttccc gatcccgctc
      661 ggggagcgac ggctgctcgc cggctacacg atcgacatcc aggagcgcaa ggaggcggag
      721 aagcggcggc tggaggccga gaggcgcttc cggctcctcg ccgaggcgac ctcggagggc
      781 gtcgtcctga tcgacgaagg ccggatcctg gaatgcaacg cccgcttcgc cgccatgcac
      841 ggcttcgagc cggccgcgct tctcgggctc cccttcctcg cgctcgtcgc gccggagtcg
      901 ctcgagcacg tctccgctcg tcacgtgtcg gaaacgacgg gccgccatcg ctggatagcc
      961 ctccgcgccg acggctcgac gttccccgtc gagggggacg cccggctcgt cccctgggaa
     1021 gggagaaccg tgaggatcaa ggcgtgccgc gacctcacgg aggagctgcg cgcggccgcg
     1081 gaggtcgcgc gcgtcgcccg gctctacgcc gccctgatcg aaatcaacga ggcgatcgtg
     1141 agggtccgga cgcccgagga gctcttcgcg gaggtccccc ggatcctcgt cgagaccggc
     1201 ggattccgca tggcctggat cggcctgaag cgggaggatt cgccgcgcgt cgacgtcgcc
     1261 tcccggttcg gggacgagac gggctacctt gcgggcatcg aggtccgcgt cgacagccgg
     1321 cccgagggtc tcggaccgac cggccgggcg atccgcgagg ggcggaccca ggtctgcaac
     1381 gacttcctga acgacccctc gaccctcccg tggcgcgatc gcgcgaagca ggcctcctgg
     1441 cgcgcctcgg cggccctccc gatccggcgg agaggactcc ccgtcggggc gctcaccgtc
     1501 tacgcgctcg aggtcggcta cttcggagag cgagagcggg cgctcctcga ggagtccgcg
     1561 gccgacgtct cctacgccct cgagcacctc gaccaggccg cggagacgcg gcgcgccgag
     1621 gcggaggtgc gggagctgaa ctcggagctg gagcgccgcg tcgaggagcg gacggcacag
     1681 ctcgaggcgg cgatccgcga gctcgaggcg ttctcctatt ccgtctcgca cgacctgcgc
     1741 gccccgctcc gcgcgatcga gggcttcgcc ggcatgatcg tcgaggacgc cgccggagcg
     1801 ctgccggagg agaccctccg acggctggac gtgatccgaa cgaacgcccg gcggatgtcg
     1861 gcgctcatcg acgccctgct gcgcttctcc cgcctgggcc gccacgagct ccagcgggac
     1921 cgggtcgaca tggcggggct cgcgcggtcg gccttcgaag aggcggtctc cgacggggcg
     1981 gcgcgcgcgc ggatcgagct ccgcatcggc gagctgccgg acgccgaggg agacgcctcc
     2041 ctcctccgcc aggtgtgggt caacctgctc gggaacgcgg ccaagtactc cgccgggcgg
     2101 gagcggccgg tcgtggaggt gacggccgtc ccgggcgacg cggagacgac ctacagggtg
     2161 agggacaacg gggtcggatt cgacccgagg tacgtcgaga agctctttcg ggtcttcgag
     2221 cggctccatg cgcccgacgc gttcgagggg accgggatcg gcctcgcgct cgtgaagcgg
     2281 atcgtccaga ggcacggcgg ggaggtctcg gccgaagggc gagagggcga gggcgcctgc
     2341 ttctccttct cggtgccgtc ggcgtccgga aaggcccgga agcggcggac gaagtagcgg
     2401 tccccctctc ctccgccgcc gctcccgcgg tgggcggcag gctttgccgt caggccgccc
     2461 ggcccggcag gtagagcgag cgggccgtca ggatccggtg gtcgaggccc gcggcccggt
     2521 cgagccgggc gaccacggac gggagcgtcg ccggaaggcg ccagaggtcg tcccggccgc
     2581 cgtccgcgac gaacgccgcc gcccactcgg tccaccacgg caccgggcgc gcggcgatcg
     2641 gctcgacgac gaggacgcgg gcgccccggc gggcggcttc gaggagccgc gggagcagcg
     2701 ccgcacgcga agtggtctcc agctcgttga cgacccatcc caggaggatc cccgcgccgc
     2761 gaccggggag gcgctcgcgc aggaggtccc ccgccgtcgc gccgccgcgc agggaaagcg
     2821 cggcgtacgt ccggcgtgcc tcgctcgccg cccagccgct cttctccact ccgaagacct
     2881 cggcgcccgc ggcgagcgcc caggccgcgc ccgccgctcc cgtaccgcac ccgatgtcga
     2941 ggacgcgcga gaggccgggc tccgccgcgc ccagggcggc gacgatcctg ccgacgacga
     3001 ggtggtggag cggaccgtag aagatggcga aggcggcccg cttcccctct ccgtcgaaga
     3061 ccgccccggc gccgatgcgt ccccggcgct cgacgtagag cgacgagacc gcctggagcc
     3121 ccttgcgcac ctgggcgaag gagagctcgg cgaggtgacg ccgctcgagg tcctcgagcc
     3181 agcggtcgaa gggagagccg tccattccct cgctcctccg cccgacgtca gggcgctccg
     3241 agctccttcg cgacggcggc gaccgcggcc tcgcccgcgg cgagggccct ctcggcggcc
     3301 gacgcggaga gcttcgccgc gtcggcgggc gccggacgcg agcgcctcca cgccccccgc
     3361 gccgcctcga cctcggcccg cgtcgcctcc tccgccgcga ggaggccggg aagcgcgcgc
     3421 gccttcaccg ccgcgaggac gcggcggtcc atgtaccgct tcttctcgcc gcgcgcgtcc
     3481 gggtagagcc tcttcctcag ctcgagggcg ctcgccgcga gaggcgccgg gccccggtcc
     3541 gcggccagcg cagccggcac cacgccggcg agaggaaaga ccggcacggc ggcccccgcg
     3601 agcgggaggc tggggacgat ccagagcgcc gccgcctcgg gcccttctcc cggcgcgggc
     3661 gcctcgagga cgatcgcgga gacggtgtcg agacggcaga cgctgtcgcc cgtgtagacg
     3721 agcgccgggc ccgcctcctc acccgtctgt ccgatcccgg cgttggcgac gtcgcgcgcg
     3781 gcgcgcagga tcgccgcaag gtcgagcccg cccgcggccg ccggcccgag gagctcgatc
     3841 gcgcgcgcct cgcgcaggaa gccggtaccc gactccttcg ctccgctgcg gctgtagttc
     3901 gagcggacga ggaggccccg cccgccgtcg ctcgacgggg cggcgtcgac gcgaacgtag
     3961 cccttcgccg acgtctcgaa gacggccgcg cctcccttcg cgtcgatgac gccgaagttc
     4021 gcggagacgt cccggccgcc ggcgttcgag cgcacgagga gcgcctcgaa ttcgtcgacg
     4081 gtggcgcacg actggagggc gagcttcatg aacgtcccct cggccgccgt gtccccgctc
     4141 tcgaggttgt aggaggccga gttcatcacc ccgaagccgg cggagttgat cccggcccag
     4201 acctggagcc ccgccgcgtc tccgcggttg acgacgccga cgtaggggta gcggccgtcg
     4261 gcgaggaaga cgacctggtt gtcgcttccg tccgcgtccc ggttcttcca gagcatcggg
     4321 cggccgctcc tcgacgccga aggggcgacg acggcagtcg tacaggcgtc cgcgggagcg
     4381 ggggcgaggg gcgcggccag gagggcgagc gcggcggccg aggcgagccg ggagcgggtg
     4441 gcgtgaacgg acacggagtc ctcctcgacc ggccatcgta gccgaggggg agccccggcg
     4501 ggtcggtcga ttcccgcccc gggctcctcc cgaagacgcg gagccggctc cccgagccgg
     4561 gcggcccgtg gcagagtgca cgcatgcagg accgtccgcg ccgcgacacc ttcctcccct
     4621 tctcccgccc gatgatcggc gaggaggaga tcgccggagt cgtcgacacg ctccgctccg
     4681 gctggatcac gaccggtccg cgggccgcgc gcttcgagga ggagctcgcg gccttcaacg
     4741 aggttccccg ctgcctcgcc ctgctgagcg cgacgaccgg cctcgagctt tcgctctccg
     4801 ccctcgggat cggcgagggg gacgaggtga tcacgacggc gcacaccttc gtctgcaccg
     4861 tctcgaacgt cgtcctccgc ggcgccgtcc ccgtcctcgt cgacatcgag cccgacacga
     4921 tgaacctcga cgcggagcgg atcgaggaac ggattacgcc ccggacgaag gcgatcctcc
     4981 cggtccacta cggcgggctc ccgtgccgca tggagcggat ctgggagatc tcccggcgcc
     5041 acggcctcgc cgtcgtcgag gacgccgccc acacgatggg ggcgcgctac gccggccggc
     5101 ggatcggctc cgacccgcgc tcggcgttct ccgtctactc gttccacccg aacaagaacc
     5161 tgacgacgat cgaggggggc gccatcgcct tccacgacct ccggcacgag gcgcacctga
     5221 agctccagcg cttccacggg atcgcccgcg gcgccggaac gccgtacccc gagggctcgc
     5281 ccctctacga cgtcccgacg ccggggcgga agtcgaactt caccgacgtc caggccgccg
     5341 tcggcctggc gcagctcccg aagctcgacg ggttcgccgc gcgccggcgc gagctcgcac
     5401 gcctctatct ctcgctcctc tcggaggtcc ccgaggtgga gctccccgcg gacggggacg
     5461 ccgagcgcga gcactgctgg aacctcttcg tcctccggaa ccgccccgag gcgacgggcc
     5521 tttcgcgcga cgagctgacg gcggcgctga aggctgagaa cgtcggcacc ggcatccact
     5581 ggcaggggct tttctccttc tcgttctacc gcgactattt cgagcggcac cccgaggggc
     5641 tcccgaagga cggcctgccg cacgcggcgc ggacgacgga gaacctgatg tcgctcccgc
     5701 tcttccccgc cctgacggag gaagacgtcc tcgacgtcgt ctcggcggtg aggcgggtcg
     5761 tcgccgccgg acggcggcgc gcgcggggct gaggcgctcc tcccggcagg ccgggacggc
     5821 ccgcgccccg cggggccgac ccgtcgtcag tccgaggagg aggcctcggc ggccggcagg
     5881 cggcgcagga tccggtagaa cgaggagcgg ctgatgccga ggaggcgcgc cgcctcgagg
     5941 cggttcccgc ccgtcttccg gagggcctcc tcgagccgcg tcggcgtgac gtcgacgatc
     6001 ggcgtccgtc cgccgcgcgg ctcggtcgcg ggcaggagca tctgcgagcg gccccagagc
     6061 gcgcggaagt ccgtcgggcc gagcaccggc ccgtcgccga agaccgccgc ctgctcgacg
     6121 gcgtgcttca gctccctcac gttcccggga aacgggtagc
//
LOCUS       DAOTEZ010000256         6157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_9131_length_6157_cov_5.205015, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000256 DAOTEZ010000000
VERSION     DAOTEZ010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6157
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9131_length_6157_cov_5.205015"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="P5164_13185"
     CDS             <1..471
                     /locus_tag="P5164_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009607864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRY44890.1"
                     /translation="MAMFDVDDYEQYVETQSESALRHIASLYGYDHGEEDEITLRSNV
                     EEVSAALKKELHERLAKAGVVVEEARLTHLAYAPEIAQAMLRRQQAEAVIAARQKIVH
                     GAVSMVDMALKELAEKHVVTLDDERKAAMVSNLMVVLCGDSEVHPVVNAGTLYG"
     gene            478..702
                     /locus_tag="P5164_13190"
     CDS             478..702
                     /locus_tag="P5164_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HRY44891.1"
                     /translation="MPSRPAAPPPPGESRKAFLLRVDPALFAEIERWAADELRSVNGQ
                     VEYLLREAVKARRRGSGRNPRPGPAGTAGA"
     gene            785..1234
                     /locus_tag="P5164_13195"
     CDS             785..1234
                     /locus_tag="P5164_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011398686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY44892.1"
                     /translation="MTTEKRRQAVLLMAEDNDDDFLLARSALEEARLLNDLRRVHDGV
                     ELLDYLRRRPPFEDPEANPLPALILLDLNMPKIDGREALSQIRNDPALCHIPVVVLTS
                     STAPEDVQQAYRLGVNSYIAKPVTFAGLVASLRILGLYWFELVVRPE"
     gene            1243..3147
                     /locus_tag="P5164_13200"
     CDS             1243..3147
                     /locus_tag="P5164_13200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRY44893.1"
                     /translation="MSSDAPLPRILVVDADADEALLVEGYLREGLGGAELPVSRALSA
                     ADARTALAADPPDVLLLDVRAHDAEAFALLEEMKVAGSPVPTIVLTGRSNAEAALHAH
                     RLGAAEVLVKPSLTPASLAATVRFATQLEESRRKEAETERALRRAERQAEALVASSLD
                     IVSILDREGRILFTSPSTEKLLGHRVETLVTRNVVEIVCPEDVEAVRAALKRCLESPG
                     TTVSVEFGVRRADGAIRLLEALTTNLLDDPGVLGLVVNSRDVTERHRSEDTLRRLEAA
                     VEQSASIIFLTDAGGTITYVNPAFTRTYGYTREEAIGSTPRLLRSGRHDAAFYAEMWA
                     TLAAGSTVKTEVWNRARDGRLVIARASIAPIRDRRGTVTGYLAVQDDVTERNVMNERL
                     RLAQKMEALGHLAREIAHELDGVLSGVLSGIDAAAALLPEGSAAAPLLGEARAAAARG
                     EALGHELLSFGRGASMELARVGANALLRDAAAQLRGRIGPGILLHLDLSPEAVVVTVD
                     RRELAHALVQLADFLLESLSPGRTLTLGTTMGPLSEPDLEALAPGSGKSWVILTVGDD
                     GTPMDEETRGRLFEPFGSSRTPGRAGGLGLAAPFGTVKQAGGFLFAAPVERGNLFRVY
                     LPPAPGADPA"
     gene            complement(3160..>6157)
                     /locus_tag="P5164_13205"
     CDS             complement(3160..>6157)
                     /locus_tag="P5164_13205"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRY44894.1"
                     /translation="QLAAERDAVARHFTLLSEASNDLVLLTDDRFRLVEVNRKACEAY
                     GYSRDELIGAEASILRPPGQEAAAREAFQRLLAEGALHLETTHRRKDGSLFPVDFSGR
                     RIEVDGRPFIQYVIRDITERKRAEDQIRRLNQLYDLLSRANAAIVRTADPAQLTRQVC
                     EIAVEVAGFRLAWISEARAEGELRAVAVAGPAAAIIDEAPLTLDPEAPSGHGPTVDAI
                     RRGAPVVANDYLSDASTTVWHEAARRFGLESLAAFPIPGPAGARGALAVGAGQMGYFD
                     DEIVTLLSGLAEDVGFALAARDREDERRRAEDALRITARRLADAEKIGRTGSFYGDLE
                     TRQVTWSEGHYLLFGETPETFAPSNDSFLERVHPDDRERMKALTSENWDGQAAGGHEY
                     RVVHRSGEVRWLNATSELVRDDSGRPVGVFGVVRDVTEQKLAEERQRLWSHVLQGSGD
                     GVLLAGADQRILLVNRAFETMTGYSAEEVLGRTPRFLGSGHHDRSFYRQMWATIARTG
                     LWRGEIQNRRSNGEVYPVLLSITAVKDDRGATSHYVAIYTDLTERIAAIERIEFLSQR
                     DPLTHFVNRAGLEAQLARAIASSRLEGHALALVTLDVDRFSAINESLGGPVGDEILRS
                     IAGRLAGAVRTRDILARIGPDEFAVGFVSVKGTAGCEIAARKVQAVFASPFEVAGRSV
                     PISASFGVSVCPEDGEDALSLMRSADAALRHAKTGEGGTLQFFRREMTARAMESLEIE
                     TKLRQAIAKGELLLHYQPQVDVETGRVVGAEALVRWKDPVRGLVPPGVFIPVAEERGL
                     IVPLGEWVLRAAAAQLRQWLDAGLPRVVVAVNVSAAQFRSPDFEGRLVGILEETGVPP
                     ELMELELTESILARDVEAAAALLGRLRSRGLSISIDDFGTGYSSLGYLRKFPLQRLKI
                     DQGFVREIWNDPSAAAIVRTVIGMSRGLGLKVVAEGVEEKRQFSFLRFHGCDEAQGYF
                     IKRPVPPEAFRAFLEAGDFDPDAPAA"
BASE COUNT          870 a         2300 c         2152 g          835 t
ORIGIN      
        1 atggcgatgt tcgacgtgga cgactacgag cagtacgtcg agacccagag cgagtcggcc
       61 ctccgccaca tcgcgagcct ctacggctac gaccacggcg aggaggacga gatcaccctc
      121 cgctcgaacg tcgaggaggt ctcggccgcg ctcaagaagg agctgcacga gcggctcgcg
      181 aaggcgggcg tcgtcgtgga ggaggcgcgt ctcacccacc tcgcctacgc ccccgagatc
      241 gcccaggcga tgctccgccg ccagcaggcg gaggcggtca tcgccgcccg ccagaagatc
      301 gtccacggcg ccgtctcgat ggtcgacatg gcgctcaagg agctcgcgga gaagcacgtg
      361 gtgacgctcg acgacgagcg gaaggccgcg atggtcagca acctgatggt cgtcctctgc
      421 ggcgactccg aggtccaccc ggtcgtcaac gccgggaccc tctatggctg aggagtgatg
      481 ccgagccgcc ccgccgcccc gccgcccccc ggggagtccc gcaaggcctt cctcctccgg
      541 gtcgaccccg ccctcttcgc cgagatcgag aggtgggcgg cggacgagct ccggagcgtg
      601 aacggccagg tcgagtacct cctgcgggag gcggtgaagg cgcggcggcg cggctcgggc
      661 cggaatcccc ggcccggacc ggccggcacg gccggggcgt gacttccggg ggcgaaagga
      721 gacaatgggg cgccgcgtgc cgccgggcct ttcgcccccg gctcccggct tcgggagggg
      781 tggaatgacg accgagaaac gccggcaggc cgtccttctg atggcggagg acaacgacga
      841 cgacttcctc ctggcgcgga gcgccctcga ggaggcgcgc ctgctgaacg acctgcgaag
      901 ggtgcacgac ggcgtcgagc tgctcgacta cctccggcgc cgtccgccgt tcgaggaccc
      961 ggaggcgaat ccccttccgg ccctcatcct cctcgacctg aacatgccga agatcgacgg
     1021 ccgggaggcg ctctcccaga tccggaacga cccggcgctc tgccacatcc cggtcgtcgt
     1081 cctgacgtcg tccaccgcgc cggaggacgt ccagcaggcc tatcgcctcg gagtcaattc
     1141 ctacatcgcc aagccggtga cgttcgccgg cctcgtcgcc tcgctccgga tcctcggcct
     1201 ctactggttc gagctcgtcg tccggccgga gtgacctgcc gcgtgagctc ggacgccccg
     1261 ctcccccgca tcctcgtcgt cgacgccgac gccgacgagg cgctcctcgt cgagggatac
     1321 ctccgcgagg ggctcggggg ggccgagctc ccggtgagtc gggcgctgtc ggccgccgac
     1381 gccaggaccg cgctcgccgc ggacccgccc gacgtcctcc tcctcgacgt ccgcgcgcac
     1441 gacgcggagg cgttcgccct cctcgaggag atgaaggtcg ccgggagccc ggtcccgacg
     1501 atcgtcctga ccggccgctc gaacgccgag gcggccctcc acgcccaccg gctcggcgcg
     1561 gccgaggtcc tcgtgaagcc cagcctgacc cccgcgagcc tggccgccac ggtccggttc
     1621 gcgacccagc tcgaggagtc gcggcggaag gaggccgaga cggagcgggc tctgcgccgg
     1681 gccgagcgcc aggcggaggc tctcgtcgct tcgtccctcg acatcgtctc gatcctcgac
     1741 cgcgagggac gcatcctctt cacgagcccg tcgacggaga agctcctcgg ccaccgcgtc
     1801 gagacgctcg tcacccgcaa cgtcgtcgag atcgtctgtc ccgaggacgt cgaggcggtc
     1861 cgcgcggccc tcaagcgatg cctcgagagc ccgggaacga ccgtctccgt cgagttcggc
     1921 gtgcgccgcg ccgacggcgc gatccggctg ctcgaagccc tcacgacgaa cctcctcgac
     1981 gaccccgggg tcctcgggct cgtcgtcaac tcccgtgacg tcacggagcg ccaccgctcg
     2041 gaggacaccc tgcggcgcct cgaggcggcc gtcgagcagt cggccagcat catcttcctg
     2101 acggacgccg gagggacgat cacctacgtc aaccccgctt tcacgcggac ctacggctac
     2161 acgcgcgagg aggcgatcgg ctccactccg cggctcctcc ggagcgggcg gcacgacgcc
     2221 gcgttctacg cggagatgtg ggcgaccctc gcggccggga gcacggtcaa gacggaggtc
     2281 tggaaccggg cgcgggacgg ccgcctcgtc atcgcgcgcg cctcgatcgc cccgatccgg
     2341 gaccggcggg gaacggtcac cggctacctg gcggtccagg acgacgtcac cgagcggaac
     2401 gtcatgaacg aaaggctccg cctcgcgcag aagatggagg ccctggggca tctcgcgcgc
     2461 gagatcgcgc acgagctcga cggcgtcctc tcgggtgtcc tctcggggat cgacgcggcg
     2521 gcggccctgc tcccggaggg gagcgccgcc gcgcccctgc tgggcgaggc ccgggcggcg
     2581 gcggcgcgcg gcgaggcgct cgggcacgag ctcctctcgt tcggccgggg cgcgtcgatg
     2641 gagctcgccc gcgtcggggc gaacgccctg ctgcgcgatg cggccgcgca gctgcgcggg
     2701 aggatcgggc caggcatctt gctgcacctc gacctctcgc ccgaagccgt cgtggtgacg
     2761 gtggaccgcc gggagctcgc ccacgccctc gtacagctcg ccgacttcct cctcgagtct
     2821 ctctctcccg gccggaccct cacgctcggg acgaccatgg ggccgctctc ggagccggac
     2881 ctggaggctc tcgctccggg ctccgggaag agctgggtga tcctgacggt cggggacgac
     2941 gggacgccga tggacgagga gacccgaggc cgtctgttcg aaccgttcgg ctcctcgcga
     3001 acgcccgggc gcgccggcgg cctcggcctc gccgccccgt tcggcaccgt gaagcaggcc
     3061 ggagggttcc tcttcgcggc gcccgtggag cgggggaacc tcttccgcgt ctacctgccg
     3121 cccgcccccg gggccgatcc ggcctgacgg gcccgcggct caggccgccg gggcgtcggg
     3181 gtcgaagtct ccggcctcga ggaaggcgcg gaacgcctcg ggggggaccg ggcgcttgat
     3241 gaagtagccc tgcgcctcgt cgcagccgtg gaagcggagg aacgagaact gccgcttctc
     3301 ctcgacgccc tccgccacga ccttcagccc gagcccgcgg gacatcccga tgaccgtccg
     3361 gacgatcgcc gccgccgaag ggtcgttcca gatctcgcgc acgaaaccct ggtcgatctt
     3421 cagacgctgc agcgggaact tccgcaggta gccgagcgag gagtagccgg tcccgaagtc
     3481 gtcgatggag atcgagaggc cgcgggaccg gagcctcccg aggagagccg ccgccgcctc
     3541 gacgtcgcgc gccaggatgc tctcggtcag ctcgagctcc atgagctccg gcgggacgcc
     3601 ggtctcctcg agaatcccga ccaggcgccc ctcgaagtcg ggcgagcgga actgggccgc
     3661 cgagacgttc acggccacga cgacccgggg cagccccgcg tcgagccact ggcgcagctg
     3721 cgccgccgcg gcgcgcagga cccactcgcc gagcggcacg atcaggccgc gctcctcggc
     3781 gaccggtatg aagactcccg gcgggaccag gccgcgcacg gggtccttcc agcggacgag
     3841 cgcctcggct ccgacgaccc gccccgtctc cacgtccacc tgcggctggt agtgaaggag
     3901 gagctcgccc ttcgcgatcg cctgacgaag cttcgtctcg atctccagcg actccatcgc
     3961 ccgggccgtc atctcgcggc ggaagaactg gagcgtcccc ccttctcccg tcttcgcgtg
     4021 ccggagcgcc gcgtccgcgc tccgcatcag cgacagggcg tcctcgccgt cctccgggca
     4081 gacggagacg ccgaagctcg ccgagatcgg gaccgaccgg cccgcgacct cgaacggcga
     4141 cgcgaagacg gcctggacct tccgggccgc gatctcgcat ccggcggtcc ccttcacgct
     4201 cacgaagccg acggcgaact cgtccggccc gatccgggcg aggatgtccc gcgtccgcac
     4261 cgctccggcc agccgccccg cgatcgagcg gaggatctcg tcgcccacgg ggccgccgag
     4321 cgactcgttg atcgccgaga accggtcgac gtcgagcgtc acgagggcga gcgcgtgccc
     4381 ctcgagccgg gacgacgcga tcgcgcgggc gagctgcgcc tcgaggcccg cgcggttcac
     4441 gaagtgggtc agggggtctc gctgggagag gaactcgatc ctctcgatcg ccgcgatgcg
     4501 ctccgtcagg tcggtataga tcgcgacgta gtggctcgta gccccccggt cgtccttcac
     4561 ggcggtgatc gagaggagga ccggatagac ctccccgttg ctccgcctgt tctggatctc
     4621 cccgcgccac agtcccgtcc gggcgatcgt cgcccacatc tggcggtaga aggaccgatc
     4681 gtggtggccc gagccgagga accgcggggt cctgccgagc acctcctccg cggagtagcc
     4741 ggtcatcgtc tcgaaggccc ggttcacgag caggatgcgc tggtcggccc ccgccaggag
     4801 gacgccgtcg ccgctcccct gaaggacgtg ggaccagagc cgctgccgct cctcggcgag
     4861 cttctgctcc gtcacgtccc gcacgacgcc gaagacccca accgggcggc cggagtcgtc
     4921 gcggacgagc tcagaggtcg cgttcagcca gcggacctcg ccggaacggt ggacgacgcg
     4981 gtactcgtgc cctccggccg cctgcccgtc ccagttctcc gaggtcagcg ccttcatccg
     5041 ctcccggtcg tccgggtgga cccgctcgag gaacgagtcg ttcgagggcg cgaaggtctc
     5101 gggcgtctca ccgaagagca ggtagtgccc ctcggaccat gtgacctgcc gcgtctcgag
     5161 gtcgccgtag aagctcccgg tccgtccgat cttctccgcg tccgcgaggc gccgggccgt
     5221 gatcctcagg gcgtcctcgg cgcgccgcct ctcgtcttcc cggtcccggg ccgcgagcgc
     5281 gaagccgacg tcctcggcca gccccgagag gagcgtcacg atctcgtcgt cgaagtaccc
     5341 catctggccc gccccgacgg cgagcgcgcc gcgcgccccc gccggacccg ggatcgggaa
     5401 cgccgcgagc gactcgagcc cgaaccgccg ggccgcctcg tgccagacgg tcgtcgaggc
     5461 gtccgagagg tagtcgttcg cgacgacggg agctcctcgt cggatggcgt ccacggtcgg
     5521 cccatgaccc gacggcgctt ccgggtcgag cgtcagaggg gcctcgtcga tgatcgcggc
     5581 ggcgggcccg gcgacggcga ccgcccgaag ctccccttcg gcacgcgcct cggatatcca
     5641 cgcgagacgg aagccggcca cctccacggc gatctcgcag acctggcggg tgagctgcgc
     5701 cgggtcggcc gtccggacga tcgccgcgtt cgcgcgggag aggaggtcgt agagctggtt
     5761 caatcggcgg atctggtctt ccgcgcgctt cctctccgtg atgtcccgga tgacgtactg
     5821 gatgaacggc cggccgtcga cctcgatccg cctcccgctg aagtcgaccg ggaagaggga
     5881 gccgtccttg cgccggtggg tcgtctcgag gtggagggcg ccctcggcca gaaggcgctg
     5941 gaacgcctcc cgggccgccg cctcctgccc gggcggacgg aggatgctcg cctcggcccc
     6001 gatcagctcg tcacgcgagt agccgtacgc ctcgcaggcc ttccggttca cctccacgag
     6061 gcggaaccga tcgtcggtga ggaggacgag gtcgttcgac gcctccgaca gcagcgtgaa
     6121 gtgccgcgcg acggcgtccc gctcggccgc gagctgc
//
LOCUS       DAOTEZ010000257         6128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_9200_length_6128_cov_6.400955, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000257 DAOTEZ010000000
VERSION     DAOTEZ010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9200_length_6128_cov_6.400955"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /gene="rny"
                     /locus_tag="P5164_13210"
     CDS             <1..876
                     /gene="rny"
                     /locus_tag="P5164_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRY44895.1"
                     /translation="GRNIRAVEAATGVDIIIDDTPEAVLLSSFDPIRREVARRALERL
                     IQDGRIHPGRIEEVVEKVRAELWEDLKKTGEAAAYEFGIADLHPELVKLLGRLKYRTS
                     YGQSVLQHSKEVAWAACHMASELKVDVAVAKRAGILHDIGKAIDREQEGTHLELGRQL
                     LKKYGESEAVIHGMECHHGDYEPRTIEAVLVNAADAISAARPGARREIIETYVKRLEK
                     LEQMTQGMKGVEKTFAIQAGRELRVVVDAKQVNDSECFWMSKDLAKRIQSELQYPGQI
                     KITVIRETRAVEYAK"
     gene            957..1742
                     /locus_tag="P5164_13215"
     CDS             957..1742
                     /locus_tag="P5164_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HRY44896.1"
                     /translation="MKLLFVGDVVGKPGRKALGLLEKVIAREGVDFTVVNVENAAGGF
                     GVTAEIFADLSKLPIDVFTSGNHIWDKKEFVPYLDDIPNLLRPANYPPGNPGRGWVVQ
                     RTPAGVPVGVLNLQGQVFMAATDSPFRVGDACLGEMTASCPELKVVLIDIHAEATSEK
                     QAVGWWFDGRASLVIGTHTHVPTADARVLPKGTAYQGDAGMTGAYEGIIGFDPKNILE
                     RFRFQTPRGMETATRDVRLCGAVVDVDETTGRARTIASLQERL"
     gene            1768..2025
                     /locus_tag="P5164_13220"
     CDS             1768..2025
                     /locus_tag="P5164_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HRY44897.1"
                     /translation="MAVDPELLAILACPACDPRPPVTLCPLEAGAAAQVVEKYRDKFR
                     DEEPVVTEGLHCAACGRVYPIVSDIPVMLVEEALSAGARAS"
     gene            2145..3230
                     /locus_tag="P5164_13225"
     CDS             2145..3230
                     /locus_tag="P5164_13225"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRY44898.1"
                     /translation="MILGAAVLSARSRGALPGPGERRSSPLFGPVALFVFLSVLSALF
                     STDVVRSVVAVKGLVSFSLVLLVALLVDDDADAHVVVDAFRLTTLYLVLRGLVDWFLL
                     GQDNVDARLSGGLSIYMTYAGLLMALALVLAARALTRGGPAPGRLADGLLAAAATVLV
                     GLTFTRSAYLGLAAGLLVLVVTARPRLGLVIPPLAVLLVLVLPSGVRERAATSFDRND
                     VTARDRLAMWRAGAVMVSERPLFGVGPGRIKALYPHYRQPGFVEPSVGHLHDNVVNVA
                     AETGIPSALAYLALVAVAALRTWPLARDRSRPGVRALARGALAANAALFVAGIFEYNF
                     GDVEVLRLMLVVLALPFAAARGTEPAA"
     gene            3240..4664
                     /gene="xseA"
                     /locus_tag="P5164_13230"
     CDS             3240..4664
                     /gene="xseA"
                     /locus_tag="P5164_13230"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRY44899.1"
                     /translation="MSFRYRMPKRAAARPAGPELPFGEGTPEAKAIPVSLFVAKLNGA
                     LRASLPDSWVKGEIGEWRVWSSGHAYFTLKDASAALPAVMWASDVARVPFRPEEGMEV
                     LARGRPDVYPRSGKLSFVVSEIQPVGAGALQLALEQLRARLAAEGLFDPARKKRLPLL
                     PRRIGVVTSRHGAAVRDVLKVLSTRFPNAHVTVWPVAVQGPAAPVEIARALDGFSRLG
                     AADVVIVARGGGSRDDLSAFNDEKVVRAVAACRVPTISAVGHEIDVTLTDLAADLRAA
                     TPSQAAELVVARREDLEARIEGLSRELTRTLKGRLLAAHADAARLVHAPALSSVPARA
                     EAFRARLSSSSRELLSAVRRLPAAYGERLLRSEAAVVAWPERAALTLGLARVAGAERE
                     AAERLARSLGGARERLGALAGALSALDPLRVLARGYSVTYLEGARAPLVDAGAAAPGD
                     TLRIVLARGTLRARVTDIPRDGEP"
     gene            4661..4948
                     /gene="xseB"
                     /locus_tag="P5164_13235"
     CDS             4661..4948
                     /gene="xseB"
                     /locus_tag="P5164_13235"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRY44900.1"
                     /translation="MTSKTKLPKGLTFEKALERLEKIVAELEEPERGLEASLELFEEG
                     VALSRFCRGKLEEIQKRVDVVLKETPDGLVTEPLDADDEKGGGEDGDEEEA"
     gene            4945..5844
                     /locus_tag="P5164_13240"
     CDS             4945..5844
                     /locus_tag="P5164_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRY44901.1"
                     /translation="MTLEAFLASARSEAEAALERVLPPPGDPLADAMRYSTLAGGKRL
                     RPALALAFHDVLGGSSAPRGEVAEAAAALELVHTYSLVHDDLPCMDDDALRRGKPTCH
                     VVHGEATALLAGDALQSLGFEVLASRPRGEAFSPRRAEAVLLLARAIGAGGMAGGQAL
                     DLAASGHAHGELRTEGRLKEIHEKKTGRLLSAACELGAVLAGASEERRRAAADYGDAL
                     GVLFQIADDLLDVTASAAVLGKSVGKDEAQEKLTYVSVYGLAGAVAERERALAVTLER
                     ARALEGEPGLLAALAGYAAHRDR"
     gene            5867..>6128
                     /locus_tag="P5164_13245"
     CDS             5867..>6128
                     /locus_tag="P5164_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011453214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HRY44902.1"
                     /translation="MTQRPTPLLDRVNWPRDLKPLTLEQLATLSDELRTFLIDSVSKT
                     GGHFASGLGTVELTVALHYVFDTPRDLLVWDVSHQCYPHKVLT"
BASE COUNT          793 a         2253 c         2211 g          871 t
ORIGIN      
        1 gggcggaaca tccgcgccgt cgaggccgcg acgggcgtcg acatcatcat cgacgacacg
       61 ccggaggcgg tcctcctctc ctcgttcgac ccgatccggc gcgaggtcgc gaggagggcc
      121 ctcgagcggc tcatccagga cggccggatc caccccggga ggatcgagga ggtcgtcgag
      181 aaggtccgcg cggagctctg ggaggacctc aagaagacgg gcgaggcggc ggcctacgag
      241 ttcggaatcg ccgacctcca ccccgagctc gtcaagctcc tcgggcgcct gaagtaccgg
      301 acgagctacg ggcagagcgt cctgcagcat tccaaggagg tcgcctgggc cgcctgccac
      361 atggcgagcg agctaaaggt cgacgtcgcc gtcgccaagc gcgccgggat actccacgac
      421 atcggcaagg cgatcgaccg cgagcaggag gggacgcacc tcgagctcgg ccggcagctc
      481 ctgaagaagt acggcgagag cgaagcggtc atccacggca tggagtgcca ccacggcgac
      541 tacgagccga ggacgatcga agcggtcctc gtgaacgcgg ccgacgcgat ctcggcggcc
      601 cggcccgggg cccggcgcga gatcatcgag acctacgtca agcggctcga gaagctcgag
      661 cagatgacgc aggggatgaa gggggtcgag aagaccttcg cgatccaggc cggccgcgag
      721 ctgcgcgtcg tcgtcgacgc caagcaggtg aacgactcgg agtgcttctg gatgtcgaag
      781 gacctcgcca agcggatcca gtcggagctc cagtacccgg ggcagatcaa gatcacggtc
      841 atccgggaga cccgcgcggt cgagtacgcc aagtagcgcc cggtcagccg aacggaagcc
      901 cgccgccccg ggggaccccc ggggcggctt cgtcagatcc cagggaggac cggatcgtga
      961 agctcctttt cgtcggagac gtcgtcggga agccggggcg caaggcgctc ggcctcctcg
     1021 agaaggtcat cgcccgcgag ggggtcgact tcaccgtcgt gaacgtcgag aacgccgcgg
     1081 gcggcttcgg cgtgacggcg gagatcttcg ccgacctctc gaagctcccc atcgacgtct
     1141 tcacgtcggg gaaccacatc tgggacaaga aggagttcgt cccatatctc gacgacatcc
     1201 cgaacctcct ccgccccgcg aactaccctc ccggcaatcc cggccgcgga tgggtcgtcc
     1261 agcggacgcc cgccggggtc cctgtcggcg tcctgaatct gcagggccag gtcttcatgg
     1321 ccgcgacgga ctcgccgttc cgggtcggcg acgcctgcct cggcgagatg acggcctcgt
     1381 gcccggagct gaaggtcgtc ctgatcgaca tccacgccga ggcgacctcg gagaagcagg
     1441 ccgtcggctg gtggttcgac gggcgcgcct cgctcgtgat cgggacgcac acgcacgtcc
     1501 cgacggccga cgcccgcgtc ctgccgaagg ggacggccta ccagggcgac gccgggatga
     1561 ccggcgccta cgaggggatc atcgggttcg accccaagaa catcctcgag cggttccggt
     1621 tccagacgcc ccgcgggatg gagacggcga cccgggacgt gaggctctgc ggcgccgtcg
     1681 tcgacgtgga cgagacgacg ggaagggccc ggacgatcgc ttcgctgcag gagcggctct
     1741 gaggccgcct gctgctagca tccgcgtatg gccgtcgacc ccgagctcct cgccatcctc
     1801 gcctgcccgg cctgcgaccc acggcccccg gtgaccctct gcccgctcga ggccggagcg
     1861 gctgcgcagg tcgtcgagaa gtaccgtgac aagttccgcg acgaggagcc ggtcgtgacg
     1921 gaggggctcc actgcgccgc ctgcggccgc gtctacccga tcgtgtcgga catcccggtc
     1981 atgctcgtcg aggaagccct ctccgccggg gcgcgcgcgt cctgaagctc ctgccggggc
     2041 ctctcgctcg ccgggcgccg ctcccgagag cggccttcgc gggcctcgtc ctctgcgccg
     2101 cgctcacgcc ctggaacgca cccgcgaacg tcgctctcgc gctcgtgatc ctcggggctg
     2161 ccgtcctttc ggcgcgctct cgcggcgctc tccccggccc gggggaacga cggtcctcgc
     2221 cccttttcgg gccggtcgcg ctcttcgttt tcctgagcgt cctctcggcg ctcttctcga
     2281 cggacgtcgt gcgctcggtc gtggcggtga aggggctcgt ctccttctcc ctcgtcctgc
     2341 tcgtcgcgct cctcgtcgac gatgacgccg acgcgcacgt ggtcgtggac gccttccgcc
     2401 tgaccacgct ctacctcgtc ctgcgcgggc tggtcgactg gttcctcctc gggcaggaca
     2461 acgtcgacgc ccgtctctcg ggcggcctgt cgatctacat gacgtacgcc gggctcctga
     2521 tggccctcgc cctcgtcctg gcggcgcggg cgctgacgcg gggcgggccg gcgcccgggc
     2581 gcctcgccga cggcctcctc gcggccgccg cgacggtcct cgtcggactc acgttcacgc
     2641 ggagcgccta cctcggcctc gccgccgggc tcctcgtcct cgtcgtgacg gcccgccccc
     2701 ggctgggcct cgtgatcccg cccctcgccg tcctcctcgt cctcgtcctt ccctccggcg
     2761 tccgcgagcg ggcggccacg tcgttcgacc ggaacgacgt gaccgcccgc gaccggctcg
     2821 cgatgtggcg ggccggagcc gtgatggtct ccgagcggcc gctgttcggc gtcggtcccg
     2881 ggcggatcaa ggctctctac ccgcactacc ggcagccggg cttcgtcgag ccgtccgtcg
     2941 gccacctcca cgacaacgtc gtgaacgtcg cggcggagac cgggatcccc tcggctctcg
     3001 cctacctcgc gctcgtggcc gtcgccgcgc tccggacgtg gccgctcgcc cgggaccgct
     3061 cccgcccggg ggtccgcgcg ctcgcgcggg gcgcgctcgc ggcgaacgcc gcgctcttcg
     3121 tggcggggat cttcgagtac aacttcggcg acgtggaggt gctccggctg atgctcgtgg
     3181 tcctcgccct gcccttcgcg gcggcgcgcg ggacggagcc cgccgcctag aatcgggcga
     3241 tgtcgttccg ctatcggatg ccgaagaggg cggcggcccg cccggccgga ccggagctgc
     3301 cgttcggcga ggggacgccg gaggcgaagg cgatcccggt ctcgctgttc gtggcgaagc
     3361 tgaacggcgc cctgcgtgcc tcgctccccg actcctgggt gaagggggag atcggggagt
     3421 ggagggtctg gtcgtccggc cacgcgtact tcacgctgaa ggacgcgagc gccgccctcc
     3481 cggcggtgat gtgggcgtcg gacgtcgcgc gggtcccgtt ccgtcccgag gaggggatgg
     3541 aggtcctcgc gcgcgggcgt cccgacgtct atccgcggag cgggaagctc tcgttcgtcg
     3601 tctccgagat ccagccggtc ggcgccgggg cgcttcagct cgccctcgag cagctccggg
     3661 cgcggctcgc tgcggaaggg ctcttcgacc cggcgcggaa gaagaggctc ccgctcctcc
     3721 cgcggcggat cggggtcgtc acgtcccggc acggcgccgc cgtgcgggac gtgctgaagg
     3781 tcctttccac gcgcttcccg aacgcgcacg tcacggtctg gccggtcgcg gtccaggggc
     3841 cggcggcgcc ggtcgagatc gcgcgggccc tggacggctt ctcgcggctc ggcgccgccg
     3901 acgtcgtgat cgtggcgcgc ggcggcggat cccgggacga cctctccgcg ttcaacgacg
     3961 agaaggtcgt ccgggccgtg gccgcgtgcc gcgtcccgac gatctcggcc gtggggcacg
     4021 agatcgacgt cacgctgacc gacctcgccg ccgacctgcg cgccgcgacc ccctcccagg
     4081 cggcggagct cgtcgtcgcc cgcagggagg acctcgaagc ccggatcgag gggctctcgc
     4141 gcgagctcac gcggactctg aagggacggc tgctcgcggc gcacgccgac gcggcgcgcc
     4201 tcgtccacgc ccccgcgctc tcctcggtcc cggcgcgggc ggaggcgttc cgggcgcggc
     4261 tctcgtcgtc gtcgcgcgag ctcctctcgg ccgtccggcg gctgcccgcg gcctatgggg
     4321 aacggcttct gcgttccgag gcggcggtcg tcgcctggcc cgagcgggcg gcgctgacgc
     4381 tcggcctcgc gcgcgtcgca ggggcggagc gggaggctgc ggagcggctg gcccggagcc
     4441 tcggcggcgc ccgcgagcgc ctcggtgccc tggccggcgc cctgtcggcg ctcgacccgc
     4501 tccgggtcct cgcccgcggc tattccgtga cctacctcga gggtgctcgg gcgcccctcg
     4561 tcgacgcggg ggcggcggcc cccggcgaca cccttcgaat cgtcctcgcg cgcgggacgc
     4621 tccgcgcgcg cgtgaccgac atccccaggg acggtgaacc atgacgtcga agacgaagct
     4681 gccgaagggg ctgacgttcg agaaggcgct cgagcgcctg gagaagatcg tggcggagct
     4741 ggaggagccg gagcggggcc tcgaagcctc gctcgagctc ttcgaggagg gcgtcgcgct
     4801 ctcccgtttc tgccgcggca agctcgagga gatccagaag cgggtggacg tcgtcctgaa
     4861 ggagacgccg gacgggctcg tcacggagcc gctcgacgcc gacgacgaga agggcggcgg
     4921 cgaggacggc gacgaggagg aggcgtgacg ctcgaggcgt tcctcgcgtc cgcgaggtcg
     4981 gaggcggagg cggcgctcga gcgcgtcctc cctccgccgg gcgacccgct cgcggacgcg
     5041 atgcgttact cgacgctcgc cgggggcaag cgcctccggc cggcgctcgc actcgcgttc
     5101 cacgacgtcc tcggcgggag cagcgcgccg cgcggcgagg tcgccgaggc ggctgcggcg
     5161 ctcgagctcg tccacaccta ctcgctcgtc cacgacgacc tcccgtgcat ggacgacgac
     5221 gcgctgcggc gcgggaagcc cacctgccac gtcgtccacg gcgaggcgac ggcgctcctc
     5281 gcgggcgacg cgctccagtc gctcggcttc gaggtcctcg cgtcgcgtcc gcgcggggag
     5341 gcgttctcgc cgcgacgcgc ggaggcggtc ctcctcctcg cccgggcgat cggggccggc
     5401 gggatggccg gcggccaggc gctcgacctc gctgcgtcgg gacacgccca cggggagctc
     5461 cggaccgaag gccgcctcaa ggagatccac gagaagaaga ccgggaggct cctctcggcc
     5521 gcgtgcgagc tcggcgcggt cctcgcgggc gcctcggagg agcggcgccg ggccgcggcg
     5581 gactacggcg acgcgctcgg cgtcctcttc cagatcgccg acgacctcct cgacgtcacc
     5641 gcgagcgccg cggtcctcgg caagagcgtc gggaaggacg aggcccagga gaagctgacc
     5701 tacgtctccg tctacggcct cgctggggcc gtcgccgagc gggagcgggc tctcgccgtg
     5761 acgctcgagc gggcacgggc gctggagggg gagccggggc tcctcgccgc gcttgccggc
     5821 tacgcggccc accgggaccg gtgatccgga tatcatcaaa ggttctatga cccagcgtcc
     5881 gaccccgctc ctggaccgtg tgaactggcc ccgggacctc aagcccctca cgctcgagca
     5941 gctcgcgacc ctctcggacg agctccgcac gttcctgatc gactccgtct cgaagacggg
     6001 cggccacttc gcatccggtc tcgggaccgt cgagctgacc gtcgcgctgc actacgtctt
     6061 cgacacgccg cgggacctcc tcgtctggga cgtcagccac cagtgctacc cgcacaaggt
     6121 cctgacgg
//
LOCUS       DAOTEZ010000258         6118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_9221_length_6118_cov_5.894937, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000258 DAOTEZ010000000
VERSION     DAOTEZ010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9221_length_6118_cov_5.894937"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="P5164_13250"
     CDS             complement(<1..656)
                     /locus_tag="P5164_13250"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRY44903.1"
                     /translation="MKSSPGPKALFVLPAVLLAIATFVAPPASGQTTTAQVRGTVTDA
                     QGPIPAMPVIAVNTATGARFSAATATNGGYVLVLPAATYELSVSTGAHAPWKRNLQVG
                     VGQSLTLDVALQPASIAAEVAVVATAAEAQVERVTSEVATNVSEIQIQSLPQSSRNFL
                     NFAALAPGVRLSRDELRQEYSYGAQGSSNTNVFIDGTSYKNDVLLGGAVGQDASRGNP
                     "
     gene            complement(653..1813)
                     /locus_tag="P5164_13255"
     CDS             complement(653..1813)
                     /locus_tag="P5164_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRY44904.1"
                     /translation="MARDERRARGTRSAGRTSGEAGAAAGGIREVARAAGVSVATVSR
                     VVNGTAVVRDETKRRVEAEIARLRYAPNAAARSLITNRTSTVGVVLPDIWGEYFSEVI
                     RGIDLAARQAGYHVLVSSSHSDAAETREVLRAMNGRVDGVVVMAPHASPQALASSFPP
                     ALPAVFLNSAPGGAGAPSLSVDNRGGARAMVAHLLALGHRRLAFLNGPASNRDAAERR
                     RGAHDALRAAGLATEPVLEIPGDFTEESGRVAGEEVLRRSPRPTALFAANDSMAIGAL
                     YALRRARASIPADVAVAGFDDVPIARFASPPLSTVRHDIRSLGARALARLVAEIEGRG
                     DGLPDREVLPFHLVLRYSTEGVDTETTTEPSHPLRAGAPVPYPTTDRRRKAR"
     gene            2000..3526
                     /locus_tag="P5164_13260"
     CDS             2000..3526
                     /locus_tag="P5164_13260"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY44905.1"
                     /translation="MSRRRLRAALVVLGACFASCRPRDPVPLPEPVRLARQTPVLSLD
                     PTESEPRTLSALANVFEPLVAYDRDLNLVPALATRWETPDETTWDFSLRRGVTFHDGS
                     PLDAPVVVAALERARLSRESSVPGTLWAVSHVEATDGHSIRIRTRVPDALLLHELTLV
                     LVPRGTSRAEIEARPVGTGPYRVAGWTRGGPLDLEAWDDYWGPSPFLRRARIDALPPG
                     VDGAAALASGRAEVAELSPSAARAAPPAGVRFVTTPGLTTFYLWMNGPKTIEGRPNPF
                     ADVRVRRAAARAIDRARLARAATGTNATAALQLVPPTVFGHVPSMTTLPYDPPAARAL
                     LQSAGYTLPVEAVLSYRAVGSDDPAASALREMLDEAGFRVTLRPVAWDQVQSELRGGR
                     LSLFFGGWVFDGPDAGGFLRDCIRSRGPNGRTGYFNSGYANPEVDRLVDASYGAFVRT
                     ARLTLLEEAMRIANADAPLVPLFHRPDAWAVAPGLLWEPRPDGKVLAAEMRPAGAPRP
                     "
     gene            3611..5485
                     /gene="sppA"
                     /locus_tag="P5164_13265"
     CDS             3611..5485
                     /gene="sppA"
                     /locus_tag="P5164_13265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRY44906.1"
                     /translation="MNSERPRSLPRRALSAIGRGLTFVRNTFLNVLALSVVVGLLAVL
                     FAGGPKVEKGSALVLVPKGNIVEQLAAKSPSDLVEDFAGGGTEETLLKDLVDALAAAK
                     ADERISSVYLDLDEMGGAGMTVLEDVAAALADFRSSGKKVVVAGDLFDMRRYYLAAQG
                     DELLLHPQGMVLIEGFGRYRSYYKDAIDRLGVTWNVFRVGEYKSAVEPYLRDDMSPEA
                     KEADLEWMGDLWRAYLAAVAKARKTTPEALTALVDQLPERLKASGGDPAKLALDAKLV
                     DRLATRDEVRKRMIELAGEDSKSKSFRQVALGDYLEALGNDRPGAKGKGDAVAVVVAK
                     GEIVDGTAGPGRVGGDSTAKLIRQARQDEKVKAIVLRVDSPGGSAFASEVIRRELELA
                     REAKKPVVVSMGSVAASGGYWISTSSDEIWASPETITGSIGIFGMFPTIEKPLAKYLG
                     VHVDGVGTTPWAGLRIDRELPPRAGEAIQAMIDQGYEAFLERVGKARKMTRDQVDKIA
                     RGRVWSGADAKGIGLVDHLGGLGKAIESAAAKAKLGKTYRVTYVEKEKSLKEKLLSSL
                     SVRATRLAAAFGYTLAPAAETADGPGAALLGAVAAEVDQLSVWNDPRGVYAHCLCEVK
                     "
     gene            complement(5582..>6118)
                     /locus_tag="P5164_13270"
     CDS             complement(5582..>6118)
                     /locus_tag="P5164_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44907.1"
                     /translation="PVDLRFRVQRPVARMTAQKEMVANLFPSTPWSPRRTLGSGAVAN
                     ALSSLPAPGPAAQRLADAVVVAGLEAAKGNGRRTVVLILGPKAKDEGLLGAEEARSFL
                     TRLGVPLQVWSAGRKHSREAVRWNAQRLMSDTWEVEGAVHELLETAARQRIVWVEGSH
                     LPQDVELGPLAKGIRLAR"
BASE COUNT          845 a         2282 c         2137 g          854 t
ORIGIN      
        1 aacgggttcc cgcggctcgc gtcctgcccg acggctcccc cgaggaggac gtcgttcttg
       61 tagctcgtcc cgtcgatgaa gacgttcgtg ttcgacgacc cctgcgcgcc gtagctgtac
      121 tcctgccgca gctcgtcgcg tgaaaggcgg acccccggcg cgagggccgc gaagttcagg
      181 aagttccggc tcgactgcgg gaggctctga atctggatct cgctgacgtt cgtcgcgacc
      241 tcggaggtga cccgctccac ctgggcctcg gcggcggtcg cgacgaccgc gacctcggcc
      301 gcgatcgagg cgggctggag cgcgacgtcg agcgtcaggc tctgccccac gccgacctgg
      361 aggttcctct tccagggggc gtgcgcgccg gtgctcacgg agagctcgta ggtcgctgcg
      421 ggaaggacga ggacgtatcc gccgttcgtc gcggtcgccg ccgagaatct cgccccggtg
      481 gcggtgttca cggcgatcac gggcatggca ggtatcggcc cctgggcgtc ggtgaccgtt
      541 cccctcacct gcgccgtcgt cgtctggccg gatgccggcg gggcgacgaa ggtcgcgatg
      601 gcgaggagga cggccgggag aacgaagagg gccttcggcc ccgggctgct tttcatcgtg
      661 ccttcctcct tcgatcggtt gtcggatacg gaacgggcgc gcccgcccga agagggtggg
      721 acggctcggt cgtcgtctcg gtgtcgaccc cctcggtcga gtaccggaga acgagatgga
      781 acggcaggac ctctcggtcg gggaggccgt ctccgcgtcc ctcgatctcg gccacgagcc
      841 gggcgagggc gcgcgcgccg agcgaacgaa tgtcgtggcg taccgtggag agcggcgggc
      901 tcgcgaagcg cgcgatcggg acgtcgtcga agccggcgac ggcgacgtcc gccggaatcg
      961 acgctcgcgc ccggcggagg gcgtagagcg cgccgatggc catcgagtcg ttcgccgcga
     1021 agagggccgt cggacggggg gaccgtcgga ggacctcctc gcccgccacc cgtcccgact
     1081 cctcggtgaa gtcgcccggg atctccagga ccggttcggt ggccagcccg gccgcccgaa
     1141 gcgcgtcgtg cgcgccccgc cgccgttcgg ctgcatcgcg gttcgaggcg gggccgttga
     1201 ggaaagccag acgacggtgc cccagcgcga ggaggtgggc gaccatcgcg cgggcgccgc
     1261 cccggttgtc cacgctgaga gacggggcgc ctgcgccgcc gggggccgag ttcaggaaga
     1321 cggcgggcag cgcggggggg aacgagctcg cgagcgcctg cggcgaagcg tgcggcgcca
     1381 tcacgacgac gccgtcgact cgcccgttca tcgcccggag gacctcgcgc gtctcggcgg
     1441 cgtcgctgtg agacgaggag acgaggacgt gatagccggc ctggcgtgcc gcgaggtcga
     1501 tcccgcggat cacctcggag aagtactcgc cccagatgtc ggggaggacg acgccgaccg
     1561 tcgacgtgcg gttcgtgatg aggcttcggg ccgcggcatt cggcgcgtac cggagccgcg
     1621 cgatctccgc ctcgacccgg cgcttcgtct cgtcccgcac gaccgccgtg ccgttcacga
     1681 cgcgggagac cgtcgcgacg gagacgccgg ccgcccgcgc gacctcccgg atcccgccgg
     1741 ccgcggcgcc cgcctcgccg gacgtccggc cggccgatcg ggtcccccgt gcgcgtcgct
     1801 cgtctcgggc catcgagttg taaccgttta cacaggaaac ctacctccgc cgcgctgccc
     1861 gtgtcaacag gagaatgaca cgtgcctcgg cggagccacc cgcgagcgtc gcggggagct
     1921 cctccccgct tccggacgtc ccggacgagg cgggagatgc cgtcggtgac gccggagcgc
     1981 aggccataat cgagccacct tgagccgtcg acgcctccgg gccgccctcg tcgtcctcgg
     2041 cgcctgtttc gcctcgtgcc gcccgaggga ccccgttccg ctcccggagc cggtccggct
     2101 cgcccgccag acgcccgtcc tctccctcga cccgaccgag agcgagcccc gtacgctctc
     2161 ggccctcgcg aacgtcttcg agccgctcgt cgcctacgac cgcgacctga acctcgtccc
     2221 ggcgctcgcg acccgctggg agactcccga cgagacgacc tgggacttct ctctccgccg
     2281 gggcgtgacc ttccacgacg gctccccgct cgatgcgccc gtcgtcgtcg ccgccctgga
     2341 gcgcgcccgc ctgagccggg agtcgagcgt cccggggacc ctctgggccg tttcgcacgt
     2401 cgaggccacg gacggtcact ccattcggat ccgcacgcgc gttcccgacg ccctcctgct
     2461 gcacgagctc acgctcgtcc tggtcccccg agggacttcg cgcgcagaga tcgaggcgag
     2521 gccggtcggc acgggcccct atcgtgtcgc cggctggact cgcgggggcc cgctcgatct
     2581 cgaagcctgg gacgactact ggggcccgtc gccgttcctc cggagggctc ggatcgacgc
     2641 cctccctccc ggggtcgatg gcgcggcggc cctcgcgagc ggccgggccg aggtcgcgga
     2701 gctttccccg tcggccgccc gcgccgcgcc tcccgccggc gtccggttcg tcaccacgcc
     2761 gggactgacc acgttctatc tctggatgaa cggcccgaag acgatcgagg gccgaccgaa
     2821 ccccttcgcc gacgtccgcg tccgccgggc cgccgcacgc gcgatcgacc gggcgcgtct
     2881 cgcccgcgcg gctaccggca cgaacgcgac cgcggcgctt cagctcgtcc ccccgaccgt
     2941 cttcggccac gttccctcga tgacgacgct tccctacgat ccgccggcgg cccgggctct
     3001 cctgcaatct gccggctaca ccctcccggt cgaggcggtc ctctcctacc gggccgtcgg
     3061 aagcgacgac cccgcggcga gcgccctccg ggaaatgctc gacgaggccg gcttccgcgt
     3121 caccctcagg cccgtcgcct gggaccaggt ccagtccgag ctgcgcggcg ggcggctctc
     3181 cctcttcttc ggaggctggg tcttcgacgg acccgacgcg ggcggtttcc tgcgcgactg
     3241 catccgaagc cgcgggccga acggccggac gggctacttc aactcgggct acgcgaatcc
     3301 cgaggtcgac cggctcgtcg atgccagcta cggcgccttc gtccggaccg cccgtctcac
     3361 gctcctcgag gaggcgatga ggatcgcgaa cgccgacgcc ccgctcgtgc cgctcttcca
     3421 ccgccccgac gcatgggccg tcgccccggg cctcctctgg gagccccggc ccgacgggaa
     3481 ggtcctcgca gccgagatgc ggccggccgg agcgccacgc ccctgagcgc cgacaccggg
     3541 aattgcgatc cgctctcgaa cccccgctcc cggttcggcc gtacagtccg atcgaaagag
     3601 gcaccgaacg atgaacagcg aacgcccccg atccctcccc cgtcgcgccc tctcggccat
     3661 cggccggggg ctcaccttcg tccgcaacac cttcctcaac gtcctcgccc tctcggtcgt
     3721 cgtcggcctc ctcgccgtgc tcttcgccgg cgggccgaaa gtcgagaagg gctcggcgct
     3781 cgtcctcgtc ccgaagggca acatcgtgga gcagctcgcc gcgaagtctc cgagcgacct
     3841 cgtcgaggac ttcgccggag gcggcacgga agagacgctc ctcaaggacc tcgtcgacgc
     3901 cctcgcggcc gcgaaggccg acgagaggat ctcgtcggtc tacctggacc tcgacgagat
     3961 gggcggcgcc ggcatgacgg tcctggagga cgtcgccgcc gcgctcgccg acttccgctc
     4021 ctcggggaag aaggtcgtcg tcgcgggcga cctcttcgac atgcgccgct actacctcgc
     4081 cgcccagggt gacgagcttc tccttcaccc gcagggaatg gtcctcatcg agggcttcgg
     4141 ccgctaccgg agctactaca aggacgccat cgaccgcctc ggcgtcacct ggaacgtctt
     4201 ccgggtcggc gagtacaagt cggccgtcga gccctacctg cgcgacgaca tgtctcccga
     4261 ggcgaaggaa gccgatctcg agtggatggg cgacctctgg cgggcgtacc tggccgccgt
     4321 cgcgaaggcg cggaagacga cccccgaggc gctcacggcg ctcgtcgacc agcttcccga
     4381 gcgtctgaag gcctccggag gcgacccggc gaagctggcg ctcgacgcga agctcgtcga
     4441 ccggctcgcc acgcgcgacg aggtgcgcaa gcggatgatc gagctggccg gcgaggactc
     4501 gaagtcgaag tcgttccggc aggtcgcgct cggcgactac ctcgaggcgc tcgggaacga
     4561 ccgtcccggc gcgaagggga agggggacgc cgtcgccgtc gtcgtcgcga agggagagat
     4621 cgtcgacggc acggccggcc ccggccgcgt gggcggcgac tcgacggcga agctgatccg
     4681 gcaggcccgc caggacgaga aggtcaaggc gatcgtcctc cgcgtcgaca gccccggcgg
     4741 cagcgccttc gcctccgagg tgatccgccg cgagctcgag ctggcgcgcg aggcgaagaa
     4801 gcccgtcgtc gtctcgatgg gctccgtcgc ggcctcgggc ggctactgga tctccacctc
     4861 ctccgacgag atctgggcga gcccggagac gatcaccggc tcgatcggca tcttcgggat
     4921 gttccccacg atcgagaagc cgctcgcgaa gtacctcggc gtccacgtcg acggcgtcgg
     4981 gacgacgccg tgggcgggcc ttcggatcga ccgggagctt ccgccccggg ccggcgaggc
     5041 gatccaggcg atgatcgacc agggctacga ggcgttcctc gagcgcgtcg ggaaggcccg
     5101 gaagatgacg cgcgaccagg tcgacaagat cgcccgcggc cgcgtctgga gcggcgccga
     5161 cgcgaagggg atcggcctcg tcgaccacct cggcggcctc ggcaaggcga tcgagtcggc
     5221 ggcggcgaag gcgaagctcg gtaagaccta ccgcgtgacg tacgtggaga aggagaagtc
     5281 gctcaaggag aagctcctct cgagcctctc cgtccgcgcg acgcgcctcg cggctgcgtt
     5341 cggctacacg ctcgcgcctg cggccgaaac ggccgacggc ccgggagccg cgctcctcgg
     5401 cgccgtcgcg gcggaggtcg accagctctc cgtctggaac gacccgcgcg gcgtctacgc
     5461 gcactgcctc tgcgaggtga agtagccccg gcgtccggtg cgggccgagc gagcccgccg
     5521 ctccgatcga cccgaaggcc ccgccgcatc atcgcggcgg ggccttcctc ttcaaggcac
     5581 gtcagcgcgc gagccggatc cccttcgcga gcggcccgag ctccacgtcc tgcggcaggt
     5641 gagacccctc cacccagacg atccgctgtc gggcagccgt ctcgaggagc tcgtgaacgg
     5701 cgccctcgac ctcccaggtg tcggacatca ggcgctgcgc gttccaccgg accgcctccc
     5761 gcgagtgctt tcgaccggcc gaccagacct gcagcgggac gccgagccgg gtcaggaacg
     5821 accgcgcttc ttcggcaccg aggaggccct cgtccttcgc cttcggcccc aggatcagga
     5881 cgaccgtcct ccggccgttc cccttcgccg cctcgagccc cgcgacgacg acggcgtcgg
     5941 ccagccgctg agcggctggt cccggcgcgg gcagagaaga gagggcgttc gcgaccgccc
     6001 ccgaccccaa cgtcctgcga ggggaccagg gggtcgacgg gaagaggttc gcaaccatct
     6061 ccttctgggc cgtcatccgg gcgacgggac gctgcacgcg gaaccggagg tcgacggg
//
LOCUS       DAOTEZ010000259         6109 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_9246_length_6109_cov_3.923026, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000259 DAOTEZ010000000
VERSION     DAOTEZ010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6109
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9246_length_6109_cov_3.923026"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1531)
                     /locus_tag="P5164_13275"
     CDS             complement(<1..1531)
                     /locus_tag="P5164_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44908.1"
                     /translation="MTDRVDLLEERVAALSAAVAALEGRLGALEGRPAAARPAEAGAE
                     AAAARPEADEATALPTVAGSAPAVLAGRAFLGVGGAFLFRTLTEAGTLPHAAGVALGL
                     TYAAAWILAADRSGRAGRRADAVLHSFLGLAVSLPLLWESTARLQALSPGAAAGLLAA
                     VGVAVFSVAWRHDLASAAWAATLGAVGTAWALLLATARIELFTVLLIGFGAATLWATY
                     GRRWHGLRWPAALGADLAVLALVTLGARPGGPPAAWTGLSPGRAVAVALLLFVVYAGS
                     FAARTLARRRDVNVFEVFQTAAVLLVGYGGAVRLAQAAGSGERALGVAALVLAALAYG
                     ASFGFVSREAPGARNFAFFSSLAIVLALTGGGLLASGPTLAVGWTLFGLAAALLGVRF
                     GRRSLLVHGAGYLAAASVPSGLVGAGLAAFSGPGAPLPSPPGAAAGGVALVAAACWAV
                     SVGSRRSGLKDGAAWAPRTLLLAIAAAGAGAALVALSVRALPPGSLAPVRATVFAAAA
                     VG"
     gene            complement(1554..2741)
                     /gene="nrfD"
                     /locus_tag="P5164_13280"
     CDS             complement(1554..2741)
                     /gene="nrfD"
                     /locus_tag="P5164_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HRY44909.1"
                     /translation="MHGDLTTLVQGFMYPNEVELQWSVLIVLYPYITGLVAGAFILAS
                     LERVFRVEAVKPTYRLALLTALAFLIVAPLPLQLHLGHPERSFQMYLTPHTQSAMAMF
                     GFVYLWYLMAVLVLEIWLEYRAEIVRTAQESAGLKRQLYRAMALWSDDVSEEARRLDD
                     RIGYVLTIVGIPSAFLLHGYVGFIFGSIKANPWWSTPLMPIVFLFSAIVSGIALVLLM
                     YMATCVAKRIPIDMRCVDTIAKYLFYAFLIDFALEGIDLLHRMYEADESFKSLDFMVK
                     TKLFVSQILLQVVFGGLLPITLLALTQVAKLTDGARKAIYALSGVLTLVGIFAMRWNV
                     VIGGQLFSKSFLGYTTYKMSFAHREGLLPAILLMLLPFAILAVLVKLLPPWHEPEADP
                     RRA"
     gene            complement(2752..3516)
                     /locus_tag="P5164_13285"
     CDS             complement(2752..3516)
                     /locus_tag="P5164_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRY44910.1"
                     /translation="MTVDRRQLLAGLGKLLVLTPAAAAAWEYLEAGRPEEAPSYRLAD
                     HWWAMVIDVEACIGCGSCVRACKTENGVPLEPYYFRTWVERYHVPNGVSEHPEVDSPN
                     GGYDGFPERYRPGDGAKSFFVPKLCNHCAHSPCTQVCPVGATFDSPDGVVLVDKSYCL
                     GCRYCVQACPYGCRYIDPRTNTVDKCSLCYHRITKGLTTACCETCPTDARRLGDLKDP
                     KDPIHEFLRAHKVQVLKPQMATGSKTWYAGLDGSVR"
     gene            complement(3513..3974)
                     /locus_tag="P5164_13290"
     CDS             complement(3513..3974)
                     /locus_tag="P5164_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRY44911.1"
                     /translation="MAGLFVVGLALFLVARKVLVPVGFGELGHFRTGAIADNRAPKLV
                     HAGRAACAECHSDEPDRLKSGAHRGVGCEACHGALGAHAAAETDVKPARPDTGTLCLG
                     CHRQSVSKPAGFPQVDPKEHGDGSGSCGGCHDPHAPAEEPKAPPAEEAKKP"
     gene            complement(4209..5450)
                     /locus_tag="P5164_13295"
     CDS             complement(4209..5450)
                     /locus_tag="P5164_13295"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRY44912.1"
                     /translation="MTDFTFLCNECGARHPEDRSLYVCPACARLQEPGSSTRGVLRVE
                     LAEGALPRRWPDAPLASPEGLAPFLPVRGPESLFSFAAGGTPLLPVPRLREALRMPRL
                     FLKDDTRNPSGSTKDRASALVVAKALEYGYETVAAASTGNAATALSAAGAASGVKTVV
                     FVPASAPPAKLVQMASYGATLVPVAGTYDQAFELSLAACARFGWYNRNTALNPFTVEG
                     KKTASLEIARDLGPEAPDVVVVPTGDGVIVAGIAKGFADLVRCGLLPRVPRLVAVQPE
                     RSGAIARALRSGAAEITPEPDAGSVADSLTVAAPRNAVMALADVRASGGCGVLVSEEE
                     ILDAIPRLAAATAVFAEPAAAIALAGLELALAEGTVGRGERVVLLVTGTGLKDVPAAA
                     RRVRVPDPVAPTLEAVEAYLG"
     gene            5563..>6109
                     /locus_tag="P5164_13300"
     CDS             5563..>6109
                     /locus_tag="P5164_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44913.1"
                     /translation="MGRIVQSVATSYARGCLVGGLATIYAFAILVGFLLLPLGRMHRT
                     PGSDPKEIFLPLGIGAFLLLVLPALVAYRVARSRNATFDRMFGSLGLAGFPYAVQYRR
                     YEGTYGGRRFQGFFSRGPRFALEADVAVRTRFGITAGSGDTKLLAALTGEKPIRFAVP
                     AFDGLDVFGEEEAWVRRVLSEP"
BASE COUNT          904 a         2156 c         2303 g          746 t
ORIGIN      
        1 ggccgacggc cgcggcggcg aagaccgtcg cgcggacggg cgcgagcgat cccggcggca
       61 gcgcccgaac ggagagggcg acgagcgccg cgcccgctcc cgccgcggcg atcgcgagga
      121 ggagcgtgcg cggagcccag gccgccccgt ccttcaggcc agagcggcgc gatccgaccg
      181 agacggccca gcacgcggcc gcgacgaggg cgacgccgcc cgcggccgcg cccggcggag
      241 acgggagcgg cgcgcccggg cccgagaagg ccgcgaggcc ggcgccgacg agcccggagg
      301 ggacggatgc cgcggcgagg tagcccgccc cgtggacgag gagcgaacgg cggccgaaac
      361 ggacgcccag gagcgcagcg gccaggccga agagcgtcca gccgacggcg agggtcggac
      421 cggaggcgag gaggcccccg cccgtcaggg cgaggacgat cgcgagcgac gagaagaagg
      481 cgaagtttcg cgcgcccggc gcttcccgcg agacgaaccc gaaggaggcg ccgtaggcga
      541 gcgccgcgag gacgagcgcc gcgacgccca gcgcccgctc gcccgagccc gcggcctgcg
      601 ccaggcgcac cgccccgccg tagccgacga gaaggaccgc agcggtctgg aagacctcga
      661 agacgttcac gtcccgccgc cgggcgagcg tccgggccgc gaagctgccc gcgtagacga
      721 cgaagaggag aagcgcgacg gcaaccgcac gtccgggcga gagaccggtc caggccgcgg
      781 gcggcccgcc ggggcgggcc ccgagggtga cgagggcgag gaccgcgagg tcggcgccga
      841 gcgccgcggg ccagcgcagg ccgtgccacc gtctcccgta cgtcgcccag agcgtggcgg
      901 cgccgaaccc gatcaggagg acggtgaaga gctcgatccg cgccgtcgcg aggaggagcg
      961 cccaggccgt cccgacggcg ccgagcgtcg ccgcccacgc cgcggacgcg aggtcgtgcc
     1021 tccaggcgac cgagaagacg gcgacgccga cggccgcgag gagcccggcc gccgcgccag
     1081 gagacagggc ctgcaggcgg gccgtcgact cccagaggag cgggagcgaa acggcgagcc
     1141 ccaggaagga gtggagcacg gcgtccgccc ggcggcccgc cctcccgctc cggtcggcgg
     1201 cgaggatcca ggcggccgcg taggtcagcc ccagcgcgac gcccgcggcg tgagggaggg
     1261 tgccggcctc cgtcagggtc cggaagagga acgcgccccc gacgccgagg aaggcccgtc
     1321 ccgcgaggac ggccggcgcg gagcctgcga ccgtgggaag agccgtggcc tcgtcggctt
     1381 ccggccgggc cgctgcggct tcggcgccgg cctcggccgg ccgcgcggcc gccggcctcc
     1441 cctcgagggc gccgagccga ccctcgagcg ccgcgaccgc cgcggagagg gcggcgaccc
     1501 gttcctcgag gaggtcgacg cggtcggtca tacgcccctc cgctcctgcg ctctcaggcc
     1561 cgccggggat ccgcctccgg ctcgtgccag ggcggcagga gctttacgag gaccgcgagg
     1621 atcgcgaacg gcaggagcat caggaggatc gccgggagga gcccctcccg gtgcgcgaag
     1681 ctcatcttgt aggtcgtgta gccgaggaag ctcttcgaga agagctgccc cccgatcacg
     1741 acgttccagc gcatcgcgaa gatccccacg agggtcagga cgcccgagag ggcgtagatc
     1801 gccttccggg cgccgtccgt cagcttcgcg acctgcgtga gggcgaggag cgtgatcggg
     1861 aggaggccgc cgaagacgac ctggaggagg atctgcgaga cgaagagctt cgtcttcacc
     1921 atgaagtcga ggctcttgaa cgactcgtcc gcctcgtaca tccggtggag aaggtcgatc
     1981 ccctcgagcg cgaagtcgat caggaaggcg tagaagaggt acttcgcgat cgtgtcgacg
     2041 cagcgcatgt cgatcgggat ccgcttcgcg acgcacgtcg ccatgtacat gagcaggacg
     2101 agcgcgatcc ccgagacgat cgcggagaag aggaagacga tcggcatgag gggcgtcgac
     2161 caccacgggt tcgccttgat cgacccgaag atgaacccga cgtacccgtg gaggaggaac
     2221 gccgagggga tgccgacgat cgtcaggacg tacccgatcc ggtcgtcgag cctccgcgcc
     2281 tcctcgctca cgtcgtcgct ccagagcgcc atcgccctgt agagctgccg cttcaggccc
     2341 gccgactcct gcgccgtcct cacgatctcc gcacggtatt cgagccagat ctcgaggacg
     2401 aggacggcca tgaggtacca gaggtagacg aagccgaaca tcgccatcgc cgactgcgtg
     2461 tggggcgtca ggtacatctg gaacgaccgc tcgggatggc cgaggtggag ctggagcggc
     2521 agcggagcca cgatgaggaa cgcgagggcg gtgaggaggg cgagccggta ggtcggcttc
     2581 acggcctcga cccggaagac cctctcgagc gacgcgagga tgaacgcccc ggcgacgagg
     2641 ccggtgatgt acgggtagag gacgatgagg acgctccact ggagctcgac ctcgttcggg
     2701 tacatgaagc cctggacgag cgtcgtcagg tcgccgtgca cgtccccctc ctcaccgcac
     2761 cgacccgtct aggccggcgt accaggtctt cgagccggtc gccatctggg gcttgagcac
     2821 ctggaccttg tgcgcccgca ggaactcgtg gatcgggtcc ttcgggtcct tcaggtcgcc
     2881 gaggcgccgc gcgtccgtcg gacacgtctc gcagcaggcc gtcgtgagcc ccttcgtgat
     2941 ccggtggtag cagagcgagc acttgtcgac cgtgttcgtc cgcgggtcga tgtaccggca
     3001 gccgtacggg cacgcctgga cgcagtaccg gcagccgagg cagtagctct tgtcgacgag
     3061 gacgacgccg tcggggctgt cgaacgtcgc gccgaccggg cagacctggg tgcacggcga
     3121 gtgggcgcag tggttgcaca gcttcgggac gaagaagctc ttcgccccgt cgccggggcg
     3181 gtagcgctcc gggaagccgt cgtacccgcc gttcggggag tcgacctccg ggtgctccga
     3241 gacgccgttc ggaacgtggt agcgctcgac ccacgtccgg aagtagtagg gctcgagcgg
     3301 gacgccgttc tccgtcttgc aggccctcac gcagctcccg cagccgatgc aggcctcgac
     3361 gtcgatcacc atcgcccacc agtggtcggc gagacggtag gagggcgcct cctcgggcct
     3421 ccccgcctcg aggtactccc acgcggccgc ggcggccggc gtcaggacga ggagcttccc
     3481 gagaccggcg aggagctgtc tgcgatcgac ggtcatggct tcttcgcctc ctcggcggga
     3541 ggggccttgg gctcctcggc cggcgcgtgc gggtcgtggc acccgccgca gctcccgctc
     3601 ccgtcgccgt gctccttcgg gtcgacctgg ggaaagcccg ccggcttgct cacgctctgc
     3661 cggtggcagc cgaggcagag cgtcccggtg tccgggcggg cgggcttcac gtccgtctcg
     3721 gcggccgcgt gcgcgccgag cgcgccgtgg cacgcctcgc agcccactcc ccggtgtgcg
     3781 cccgacttca ggcggtccgg ctcgtccgag tggcactccg cgcaggccgc ccgtcccgcg
     3841 tggacgagct tcggcgcgcg gttgtccgcg atcgcgccgg tccggaagtg gccgagctcc
     3901 ccgaagccca cggggacgag caccttcctc gcgacgagga agagggcgag cccgaccacg
     3961 aacaggcccg ccatccggaa caggtgtgca gcaggaccaa agggtcgact catcgccgcc
     4021 tcgcccctcc ccacccggtg ataggcccaa tctggtccgg attcgcgtcg gcgccctgac
     4081 gtgaacgcgt cattccgtct gattcggatt gaccaggacc ggaccggggc cgcgtggcga
     4141 gacgggtccc gtcgccgccg gaggcggccc gaaatcggtg ccggcgcgcg gggaggcgcc
     4201 gacgccgttc agcccagata cgcctcgacg gcctcgagcg tcggcgcgac cggatccggc
     4261 acgcggaccc tccgcgcggc ggccggcacg tccttcagac cggtcccggt gacgaggagg
     4321 acgacgcgct ccccgcggcc cacggtcccc tccgcgagcg cgagctcgag gcccgcgagc
     4381 gcgattgccg ccgcgggctc ggcgaagacg gccgtcgccg ccgcgaggcg cgggatcgcg
     4441 tcgaggatct cctcctcgga gacgaggacc ccgcagccgc ccgaggcgcg gacgtccgcg
     4501 agcgccatca ccgcgttccg cggcgcggcg acggtgaggc tgtcggcgac gctcccggcg
     4561 tccggctcgg gcgtgatctc cgccgcgccg gagcggaggg cgcgggcgat ggcgccggag
     4621 cgctcgggct ggacggcaac gagccgcggg acacgcggga ggagcccgca gcggacgagg
     4681 tcggcgaaac ccttcgcgat ccccgcgacg atgacgccgt ccccggtcgg gacgacgacg
     4741 acgtccggcg cctccggccc gaggtcgcgg gcgatctcga gcgaggcggt cttcttcccc
     4801 tcgacggtga aggggttgag cgccgtgttc cggttgtacc agccgaagcg cgcgcaggcc
     4861 gcgagggaga gctcgaaggc ctggtcgtac gtccccgcga ccgggacgag cgtcgccccg
     4921 tagctcgcca tctggacgag cttggcgggc ggggccgagg ccgggacgaa gacgaccgtc
     4981 ttcacccccg acgccgcccc ggccgcggac agggccgtcg cggcgttgcc cgtcgaagcg
     5041 gcggcgacgg tctcgtaccc gtactcgagc gccttcgcga cgacgagggc agaggctctg
     5101 tccttcgtcg agcccgaggg gttccgggtg tcgtccttca ggaagaggcg cggcatccgg
     5161 agcgcctcgc gcagccgcgg caccgggagg agcggcgtgc cgcccgcggc gaacgagaag
     5221 agcgactccg ggccgcggac cgggaggaac ggggcgagcc cttcgggaga ggcgagcgga
     5281 gcgtccggcc agcggcgcgg gagggcgccc tcggcgagct cgacgcgaag gacgccgcgc
     5341 gtggaggagc ccggctcctg cagccgcgcg caggcgggac agacgtagag cgaacggtcc
     5401 tcgggatggc gggctccgca ttcgttgcag aggaacgtga agtcggtcac ggccgggcat
     5461 cgtagcgcgg accgatcgcc ggaggcgcct tcggcgcggc gtagaattgc tctttggcag
     5521 accgtccgct ccttttgcac gtttccgtcc gaggaggccg tgatgggtcg aatcgtccag
     5581 tccgtcgcga cgtcgtacgc ccgcggctgc ctcgtcggcg gcctcgccac gatctacgcc
     5641 ttcgcgatcc tcgtcgggtt cctcctgctc ccgctcggaa ggatgcatcg cacgccgggc
     5701 tccgacccga aggagatctt cctgccgctc gggatcgggg ctttcttgct gctggtcctg
     5761 ccggcgctcg tggcgtaccg ggtggcgcgc tcgcgcaacg cgacgttcga caggatgttc
     5821 ggctctctcg gcctcgccgg cttcccgtac gccgtccagt accgccgata cgaggggacg
     5881 tacggcggtc gccgtttcca gggcttcttc tcgcgcggcc cccggttcgc gctcgaggcc
     5941 gacgtggccg tccggacgag gttcggcatt acggcgggct cgggcgacac gaagctcctc
     6001 gcggcgctga cgggcgagaa accgatccgg ttcgccgtcc ccgctttcga cgggctcgac
     6061 gtcttcggcg aggaggaggc gtgggtgcgc cgcgtccttt ccgagccgg
//
LOCUS       DAOTEZ010000260         6106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_9248_length_6106_cov_6.201454, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000260 DAOTEZ010000000
VERSION     DAOTEZ010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9248_length_6106_cov_6.201454"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1697
                     /locus_tag="P5164_13305"
     CDS             <1..1697
                     /locus_tag="P5164_13305"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRY44914.1"
                     /translation="TRIMSEALRRSHPALLGALAGLGAGNVLLALNPHLLAPFPALRL
                     LSACGLLGLLLLAPFGLLVREPGTPSTGFWGFSAIVLALLAVHAETQRKLYYDFLGNG
                     TRRLLVGVALAGAVSAAAALAAARKRPRGRRAAAFLLGPAALLSAVPLLGRHGPERSP
                     LAPPQAIPRTATRSLLVVGLEGVSWELLSRAASDGSLPVFARLLEEGAGGPLGTLSPH
                     DRAALWTTAATGKMPRKHGVVSETSLPTPVGVLRLLPRVPGFARPIPLPFARPRRDGG
                     ATRRSRAFWEVLAERGHEVAVLSWPAADPPREGLVLWATERFFSESGAKGEALPEETG
                     ERARLLLLGAGNLDRPLTRALEPTGLSDAELREAHAAAGAARDLGIVGATLAGVPTGP
                     GSVSALVLSGLGGPARVFGPAADPRRYFGVSRRETDAAEKALLAYYRFLDDTLGDLLE
                     REGRERTLCVFAPASFGPAPPLTALLDLLRGRAPIASPDGGDDGFLVLWGSGIRAGVR
                     LTSADVVDLAPTLLALAGEPIARDMDGRVLAEAFDDRFSRGTSIPVVETFEPEGPQ"
     gene            1694..3538
                     /locus_tag="P5164_13310"
     CDS             1694..3538
                     /locus_tag="P5164_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44915.1"
                     /translation="MTAPARRLAAGAALLAFLLAGAALRLHRVREAPPGPWIDEALAL
                     RAARIASATSAPLLGTSPLQPPDAGFVNSWVTNLSLHGLSAIDRAAGGGIASVRAMSV
                     LPALVLLAGIAALAAAAAAGRPFPLLLAAFLGGTSSWLLVTGRWGWNAVATSAVVVLA
                     AALALLAASRRSAPLGAAAGALAGLAGWGYVAAWAILPLPPALWLLGRHRERRAATGG
                     AGDAAAFRRVATAGLVACGLVLAPLAAHYAAHPGRALARTRELSAARDGTKSALPGLA
                     RNAAAYARLFTFGGDPNERHGDPGRPVLPLAVTGLALVGVAAGLRRGGPERFLAATAG
                     LFLLASLLAVEEAANAYRAVHAAPFLVVLAALGAERLVGLADGRRRPATAAVVMVVAV
                     AGLTDAAAFLRWLASPRLEGAFGGPERALADAIRDDLGVRGPADVVLAPAAARNAFVV
                     DALLTRPGSGRPAIRLADGLGSLRYRPSRDLLLAESAASARPVPAGSLAVATGGALPG
                     FPGWRLLRVPAGAAAAAAAASLGALPLLPAPGRGGLLVVEEGLYTFSSRGGLEARLDG
                     GVLFGEARPAGSLTVRLSPGRHEVRTRLLRPGAVLRVTGPDGFVLPAP"
     gene            complement(3554..4480)
                     /locus_tag="P5164_13315"
     CDS             complement(3554..4480)
                     /locus_tag="P5164_13315"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY44916.1"
                     /translation="MKRALPPLALAAVLLAALGAGAFFADPIGLVADAGALRFRAAGA
                     RATTVPGPSGTRLFARELGPRSAETPVVLLHGLGAASHYWVETAIDLSRAGRTVIVPD
                     APGSGQSDPPAPETGWGIPGRVAAVSALLDALGIRKADLVGHSLGGWTAGAFALAEPH
                     RVRRLVLVDAAGFSKPDPRAEAELRASLSPGDRAGARRLYDLLFLRKPFPASGFLLDA
                     FGRQYRSGSVVATVAALEEKDGLLGREAELPGGTVFIHGAEDLLCPLPDTRAAFARAR
                     GARLFVIRGTGHDAPLEAPRLFRETLRAALAQ"
     gene            complement(4547..>6106)
                     /locus_tag="P5164_13320"
     CDS             complement(4547..>6106)
                     /locus_tag="P5164_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRY44917.1"
                     /translation="KLPVLFLVEDNKFAISTPVEVNTPGGSISKVVRGYPGLFLAEVD
                     GCDFLASYDALRWAIAYCTERKGPALVHAHVVRPYSHSLSDDDRLYRTEEERKKDAEG
                     DPLVRLEALLLEHGILDAERIAALKAEVDAEVNAAADAAIESPQPEAAELFEHVFSPD
                     VDPTSPAFDTEDAPSFTGTPTTMVDLINAALKDEMQRDGRIVVFGEDVADATRAEVLA
                     ECKGKGGVFKVTAGLQKAFGGDRVFNSPLAEATIVGTAIGWAARGLKPVVEIQFFDYI
                     WPAMQQLRDELATIRWRSGGTWKAPVVVRVAIGGYLTGGGPYHSQSGEVTFAHIPGLR
                     VAYPSNALDANGLLRTAIRCDDPVLFLEHKHLYRQTHNKGANPGPDYMVPFGKAKVVR
                     PGTDATLVTYGATVFRSVVAARKIAEDSGKEVEVIDLRTLAPYDFEAIARSVGKTSRL
                     LVVHEDWKTHGFGAEVAARASDELFERLDAPVRRVGAKDVFCGYAPQLEDATLPQSAE
                     IEQALRELLAY"
BASE COUNT          639 a         2373 c         2260 g          834 t
ORIGIN      
        1 ggacgcggat tatgagcgag gccctccggc gcagccaccc cgcgctcctc ggggccctcg
       61 cgggcctcgg ggcggggaac gtcctgctgg cgctgaaccc gcacctcctg gcaccgttcc
      121 cggccctgcg cctcctctcg gcctgcggcc tcctcgggct cctcctcctc gcgccgttcg
      181 gccttctcgt ccgggagccg ggaaccccgt ccaccgggtt ctggggcttc tccgcgatcg
      241 tcctcgccct gctcgccgtc cacgccgaga cccagaggaa gctctattac gacttcctcg
      301 ggaacggcac acggcgtctc ctcgtcggcg tcgcgctcgc cggcgccgtc tcggccgcag
      361 ccgccctcgc ggcggcgcgg aagaggccgc gcggccggcg cgccgccgcg tttctcctcg
      421 gcccggccgc actcctgtcg gcggtgccgc tcctcggccg ccacggcccc gagcgctcgc
      481 ccctcgcccc tccgcaggcg atcccgagaa cggcgacccg gagcctcctc gtcgtcgggc
      541 tcgagggggt ctcgtgggag ctcctttcgc gcgcggcgtc ggacggaagc ctccccgtct
      601 tcgcccggct cctcgaggag ggggcgggcg gtccgctcgg cactctttct cctcacgacc
      661 gggccgccct ctggacgacg gccgcgaccg ggaagatgcc gcgcaagcac ggcgtcgtct
      721 cggagacgtc gctgccgact cccgtcggag tcctgcggct gctcccgcgc gttcccggat
      781 tcgcccggcc gatcccgctg ccgttcgcga ggccgcgccg ggacgggggg gcgacgcgga
      841 ggagccgggc gttctgggag gtgctcgcgg agcgcggcca cgaggtggcg gtcctctcct
      901 ggcccgcggc cgacccgccc cgggaggggc tcgtcctctg ggccacggag cggttcttct
      961 cggagagcgg cgcgaaggga gaggccctcc cggaggagac cggggagagg gcgcggctcc
     1021 tcctgctcgg cgccgggaac ctcgaccgtc cgctgacgcg ggccctggag ccgaccggcc
     1081 tgtcggacgc cgagctccgg gaggcgcacg ccgcggccgg cgcggcgcgc gacctcggga
     1141 tcgtcggcgc cacgctggcg ggcgtcccga ccggccccgg aagcgtctcc gccctcgtcc
     1201 tctcgggcct cggcgggccg gcgcgcgtct tcggtccggc cgcggacccg cgccgctact
     1261 tcggcgtctc ccgtcgcgag accgacgccg ccgagaaggc gctcctcgcc tactaccgtt
     1321 tcctcgacga cacgctgggc gacctcctcg agcgggaggg gcgcgagcgg acgctctgtg
     1381 ttttcgcccc ggcgagcttc ggccccgcgc ctcccctgac cgcgctcctc gacctcctcc
     1441 gcgggcgggc gccaatcgcg tctccggacg gcggcgacga cggtttcctc gtcctctggg
     1501 gctccgggat ccgagccggc gtccgcctca cctccgccga cgtcgtcgac ctggcgccga
     1561 cgctgctcgc gctcgcgggc gagccgatcg cccgcgacat ggacgggagg gtcctcgccg
     1621 aagcgttcga cgaccgcttc tcccgaggga cgagcattcc ggtcgtggag accttcgagc
     1681 cggaagggcc gcagtgacgg cgccggcgcg ccggctcgcg gcgggagcgg cgctcctcgc
     1741 gttcctcctc gccggcgccg cgcttcgcct ccaccgcgtc cgggaggcgc cgcccggacc
     1801 ctggatcgac gaggcgctcg ccctgcgtgc ggcgcgaatc gcctcggcga cgagcgcacc
     1861 gctcctcggc acctcgcccc tgcagccgcc cgacgccggg ttcgtcaact cctgggtgac
     1921 gaacctctcg ctgcacggtc tctccgcgat cgaccgggcc gcggggggag ggatcgcctc
     1981 cgtccgggcg atgtcggtcc tgccggcgct cgtcctcctc gcggggatcg ccgcgctggc
     2041 ggcggccgcg gcggccgggc ggccgtttcc gctcctcctc gccgcattcc tcggcggcac
     2101 ctcgtcctgg ctcctcgtca ccgggaggtg gggctggaac gccgtcgcga cctcggccgt
     2161 cgtcgtcctc gccgcggcgc tcgcgctcct cgccgcgagc cggcggtcgg ctcccctcgg
     2221 cgccgccgcg ggggccctgg ccgggctcgc cgggtggggc tacgtcgccg cctgggcgat
     2281 cctcccgctt cccccggcgc tctggctcct cggccgacac cgcgagcgcc gggccgccac
     2341 gggcggcgcc ggggacgcgg ccgctttccg ccgggtcgcc acggccggcc tcgtcgcctg
     2401 cggcctcgtc ctcgctccgc tggcggcgca ctacgccgcg catcccggac gggcgctcgc
     2461 gcgaacacga gagctctccg cggcgcgcga cgggacgaag tcggccctcc ccggcctggc
     2521 ccgaaacgcc gcggcctacg cccggctttt caccttcggc ggcgacccga acgagcggca
     2581 cggcgacccg ggccgccccg tcctgccgct cgcagtcacg gggctcgccc tcgtcggcgt
     2641 cgcggcgggc ctccgccgcg gcgggccgga gcggttcctc gcggccaccg cggggctctt
     2701 cctcctcgcg agcctcctcg ccgtcgagga ggccgcgaac gcgtaccggg ccgtccacgc
     2761 ggccccgttc ctcgtcgtcc tcgcggctct cggcgccgag cgcctcgtcg gcctggccga
     2821 cgggcgccgc cgccctgcca ccgccgcggt cgtcatggtc gtcgccgtcg cgggcctgac
     2881 cgacgccgca gccttccttc gctggctcgc ctcgccccgg ctcgaaggcg cgttcggcgg
     2941 accggagcgg gcgctcgccg acgcgatccg cgacgacctc ggtgtccgcg gcccggcgga
     3001 cgtcgtcctc gcgcccgccg ccgcccgaaa cgccttcgtc gtcgatgcgc tcctcacgcg
     3061 gccggggagc ggccggccgg cgatccgtct cgcggacggc ctcggctcgc ttcggtaccg
     3121 cccctcccgc gacctcctcc tcgcggagtc cgccgcgtcc gcgcgacccg tcccggcggg
     3181 gtccctcgcc gtcgccacgg gcggggcgct gccgggcttt cccggctgga gactcctgcg
     3241 tgtcccggct ggtgcggcgg ccgcagcggc ggccgcgtcc ctcggcgctc tcccgctcct
     3301 cccggccccc ggccggggag ggctgctcgt cgtcgaggag gggctctaca ccttctcgag
     3361 ccgcggcggc ctcgaggcgc gcctcgacgg gggcgtcctc ttcggcgaag cgcgtccggc
     3421 cgggagcctc accgtgcgtc tttcgccggg gcggcacgag gtccggacgc ggctcctccg
     3481 ccccggggcg gtcctgcgcg tgaccgggcc ggacggcttc gtgcttccgg cgccctgacc
     3541 cgccgctcct cgcctactgc gcgagggcgg cccggagcgt ttcgcggaag agacggggcg
     3601 cttcgagagg ggcgtcgtgc ccggtcccgc ggatgacgaa gaggcgggcg cccctcgcac
     3661 gcgcgaacgc ggctcgcgtg tcgggaaggg ggcagaggag gtcctcggcg ccgtggatga
     3721 agaccgtccc cccggggagc tccgcctcgc ggccgaggag gccgtccttc tcctcgagcg
     3781 ccgccaccgt cgcgacgacg ctcccgctcc ggtactgccg cccgaaggcg tcgaggagga
     3841 acccggaggc ggggaacggc ttccgcagga agaggaggtc gtagagccgg cgagcgccgg
     3901 cgcggtctcc gggcgagagg ctcgcccgaa gctcggcctc ggcccgcggg tcgggcttcg
     3961 aaaagccggc cgcgtcgacg aggacgagcc gacggacgcg gtgcggctcg gcgagagcga
     4021 aggccccggc ggtccagccc ccgagcgagt gcccgacgag gtccgccttc cggatcccca
     4081 gcgcgtcgag cagcgccgaa actgcggcga cgcgccccgg gatcccccag cccgtctccg
     4141 gcgcgggcgg gtcgctctgc ccggagcccg gggcgtccgg gacgatcacg gtccgcccgg
     4201 cgcgggaaag gtcgatcgcc gtctcgaccc agtagtgcga agcagcgccg aggccgtgga
     4261 ggaggacgac cggcgtctcg gccgagcggg ggccgagctc ccgggcgaag aggcgcgtcc
     4321 ccgaggggcc gggaaccgtc gtcgcccgcg ccccggcggc gcggaagcga agggctccgg
     4381 cgtcggccac gagaccgatc gggtcggcga agaaggcgcc tgcgccgagc gcggcgagga
     4441 gcaccgccgc gagggcgagc ggagggagcg cccgtttcac ggccggaggg cggccgctcc
     4501 gcgcggctcc cctctccctc ggacgccggg gcggcctcgc ctcacgtcag tacgcgagga
     4561 gctcccggag cgcctgctcg atctccgcgc tctgcgggag cgtcgcgtcc tccagctgcg
     4621 gcgcgtagcc gcagaagacg tccttcgcgc cgacgcgccg caccggcgcg tcgagccgct
     4681 cgaagagctc gtccgacgcc cgcgccgcga cctcggcgcc gaagccgtgc gtcttccagt
     4741 cctcgtggac gacgaggagc cggctcgtct tcccgacgct ccgcgcgatc gcctcgaagt
     4801 cgtacggggc gagcgtccgg aggtcgatca cctcgacctc cttccccgag tcctcggcga
     4861 tcttccgggc cgcgacgacg ctccggaaga cggtcgcgcc gtacgtcacg agcgtcgcgt
     4921 cggtcccggg gcggacgacc ttcgccttcc cgaacgggac catgtagtcg gggccggggt
     4981 tcgccccctt gttgtgcgtc tggcggtaga ggtgcttgtg ctcgaggaag aggaccgggt
     5041 cgtcgcagcg gatcgcggtc cggaggagtc cgttcgcgtc gagggcgttc gacgggtagg
     5101 cgacgcgcag gccggggatg tgggcgaagg tcacttcgcc cgactgcgag tggtagggcc
     5161 cgcctccggt caggtagccg ccgatggcga ccctcacgac gacgggggcc ttccacgtcc
     5221 cgcccgagcg ccagcggatg gtcgcgagct cgtcgcgcag ctgctgcatg gcgggccaga
     5281 tgtagtcgaa gaactggatc tcgacgaccg gcttcaagcc gcgggcggcc cagccgatcg
     5341 cggtgccgac gatcgtcgcc tcggcgaggg gggagttgaa gacgcggtcg ccgccaaagg
     5401 ccttctggag gccggcggtc accttgaaga cgcccccctt tcccttgcat tcggcgagaa
     5461 cctcggcgcg agtcgcgtcg gcgacgtcct cgccgaagac cacgatccgc ccgtcgcgct
     5521 gcatctcgtc cttcagagcg gcgttgatca ggtcgaccat cgtcgtcggg gtgccggtga
     5581 aggagggcgc gtcctcggtg tcgaacgcgg gggaggtcgg gtcgacgtcg ggggagaaga
     5641 cgtgctcgaa gagctccgcc gcctctggct gcgggctctc gatcgccgcg tcggccgcgg
     5701 cgttcacctc ggcgtcgacc tcggccttca gcgccgcgat cctctcggcg tcgaggatgc
     5761 cgtgctcgag gaggagcgcc tccagccgga cgagcgggtc gccctcggcg tccttcttcc
     5821 gctcctcctc cgtccggtag agccggtcgt cgtcggagag cgagtgcgag taggggcgga
     5881 cgacgtgggc gtggacgagg gcgggcccct tccgctcggt gcagtaggcg atcgcccacc
     5941 gcagcgcgtc gtacgaggcc aggaagtcgc acccgtcgac ctcggcgagg aagaggccgg
     6001 ggtagccgcg gacgaccttc gagatcgagc cgccgggggt gttcacctcg accggggtcg
     6061 agatcgcgaa cttgttgtcc tcgacgagga agaggaccgg gagctt
//
LOCUS       DAOTEZ010000261         6099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_9261_length_6099_cov_5.349437, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000261 DAOTEZ010000000
VERSION     DAOTEZ010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9261_length_6099_cov_5.349437"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="P5164_13325"
     CDS             complement(<1..276)
                     /locus_tag="P5164_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44918.1"
                     /translation="MRLVRALPALLTLLPLAGPAPAQEPSRKGSISARAGVEKVLVDA
                     HVLGPDSRTLLGLTPSDFVLQVGGEEVPVEDVEFVPADRSELPPPPAG"
     gene            310..2505
                     /locus_tag="P5164_13330"
     CDS             310..2505
                     /locus_tag="P5164_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HRY44919.1"
                     /translation="MGSDTSPQDRELLAGQVERVTFHSEETGFAVLRVRVRGRREAVT
                     VVGHAAAVSAGEEIRASGSWGNDPKHGPQFRAGTLTTVAPTSAEGIERFLASGLVRGV
                     GPHFARRLVEAFGTGVFEVIESAPGRLLEVPGVGPLRAERIVASWAEQRAVREIMLFL
                     HAHGVGTSRAVRIYRTYGADAIAVVSEDPYRLARDVRGIGFVTADALARRLGIPEDAM
                     VRVRAGLSHVLSTALDEGHCGLPRETLIPRAAELLGRPETQVAEALELELAERRLVPE
                     ESGGRPCVFLPALHAAERAVAERLVRLAEGALPWKRIDPEHAIPWVEARASITLSASQ
                     RSAVATFLRSKVLVLTGGPGVGKTTLVDALLRIVLDASLRIALAAPTGRAAKRLSEST
                     RSEAKTIHRLLEVDPSTGRFRRGRDNPLDADLVVVDEVSMVDVLLARALLEAVPNGAA
                     LLLVGDSDQLPSVGPGQVLRDLLSSDAVPSVRLTEVFRQAAQSRIVVGAHRVREGHLP
                     DLTNPAGTDLFFFDAREPEEAARRVVEVVSERIPRRFGLDPRRDVQVLVPVHRGPLGA
                     RALNEALGKALNPDGAPRVSRFGMELAPGDRVMQVENDYDREVYNGDLGLVSCVDPGE
                     GELRVAFDGREVTYGFDELDLLQLAWATTIHKSQGSEYPAVVIPLAMTHYAMLERNLL
                     YTAMTRGRKLVVLVGDRRAIAVAARRSSAGSRVTRLAELLAQNSRFGSM"
     gene            complement(2478..4400)
                     /locus_tag="P5164_13335"
     CDS             complement(2478..4400)
                     /locus_tag="P5164_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44920.1"
                     /translation="MAPDEALREDRRAGAEAGVGAGADALLRQVDGMRLFPWAALVLS
                     LLPPFLLLLAAPFNPDEVEYFRASRWVGEGLVPFRDFWEHHLPLHFVLLAPVAGLARS
                     AAVESLLLLRGAQLPLLIATVLVAARLLSRAGVSRAASLLGLSCFFLAVHRSVVEIRI
                     DVTMNLFLLAGLLALTREDDGGGARPAPLVFGGMLLGLACLASQRAMPAVLVALAASA
                     ASDAASANAVSWRRIARRPLAAAAGLAVTALVTLGLASISSALPELFEQCFRLNVLYE
                     KLPSGTVSGPSAVYWLARFLHRPGAVTLIVLGAAGALLGMRQARTRRLSTVVAILAAA
                     QVILLFSIRSPFPYQFQTLFWLLAILAAVTVDALMRIGRRSAVVVASAAALLALVGMT
                     AAVRAVRWPVLAETLAHQDHVLRSIDRLTRPGSVVLEGCGFAVNRRPALETWFLPSLA
                     RDLMAAGILESPTVKELESRRVALVVADSRLLSTAMTTKALGAFVARNFFPLERFVWV
                     PSPNARLQPGERTEWTILAGGAYRVVEVPHRLLHPWFESPWSFPFQRAKDGSAFRLDV
                     SKLRPPDSAEVRLLLDGVPLDSGPDGRVVLEAGARLVAENTSGLLRAVLVAPAGHRVF
                     LDAPYPSTYIEPNLEF"
     gene            4523..>6099
                     /locus_tag="P5164_13340"
     CDS             4523..>6099
                     /locus_tag="P5164_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRY44921.1"
                     /translation="MTSGGELVAEVLLRHGVRFLFTLCGGHISPILAAAKARGVRVVD
                     TRHEATAVFAADAVARLTGVPGVAAVTAGPGVTNALTALQNARLAQTPLVLLGGATAT
                     VLRGRGALQDVDQIALASPAVKRAWRVSKLRDLVPALEEAFAVARDGVPGPVFVECPV
                     DLLYPERVVRSWYSTKAGAPKGLVAKATRWYLDRHLDALFAGVDGVDARGPAPGPPPP
                     PEAGSVGTAAFLLGRSERPVALLGSGALLDPAGAGALARAVAELGLPVYLSGMARGLL
                     GAASPLQSRHRRSEAIREADLVLLAGVPCDFRLDYGRQIRPDAVVISANRDSAARALN
                     RRPTLGVGGDPGLFLRALADAVPGGFPRPEWVARLRERDAAREAEILAAPEGAPGALD
                     PVALCRSLEEALPAKGVVVADGGDFVATASYVLRPRAPLSWLDPGVFGTLGVGAGFAL
                     GAALCRPEASVLLLWGDGSAGYGLVEIDTFVRHSLGVVAVIGNDAAWTQIAREQVEVL
                     GDDVGTVLRSTDYHAAAR"
BASE COUNT          870 a         2249 c         2206 g          774 t
ORIGIN      
        1 gcccgcgggc gggggcggga gctccgaccg gtccgccggg acgaactcca cgtcctccac
       61 cggcacctcc tcgcccccga cctggaggac gaagtccgag ggcgtcaggc cgaggagggt
      121 cctgctgtcc gggccgagga cgtgcgcgtc gacgaggacc ttctcgaccc cggcccgcgc
      181 cgagatgctc cccttccgag agggctcctg cgccggcgcc ggcccggcga gggggaggag
      241 ggtgaggagg gcggggagcg cgcggacgag cctcacgaag gacattgtct cccttacgat
      301 cccctccgag tgggaagcga cacgtctccg caggatcggg agctcctcgc cggacaggtc
      361 gagcgagtga cgttccactc ggaggagacc ggcttcgccg tcctccgggt ccgcgtgcgc
      421 gggaggcgcg aggccgtgac cgtcgtcggc cacgccgcgg cggtctccgc gggcgaggag
      481 atacgcgcct ccgggagctg gggaaacgac ccgaagcacg gtccccagtt ccgggccggg
      541 acgctgacga cggtggcccc gacctcggcc gaggggatcg agcgcttcct cgcctccggt
      601 ctcgtccgcg gggtcggacc gcatttcgcc cggcggctcg tcgaggcgtt cgggaccggc
      661 gtattcgagg tgatcgagtc cgcccccggg cggctcctcg aggtgccggg ggtcggcccc
      721 ctgcgcgcgg agcggatcgt cgcgagctgg gccgagcaga gggccgtccg ggagatcatg
      781 ctcttccttc acgcgcacgg cgtcggcacc tcgcgcgccg tgaggatcta ccggacctac
      841 ggcgcggacg cgatcgccgt cgtctcggag gatccatacc gcctcgcccg cgacgtccgc
      901 gggatcgggt tcgtgacggc cgacgccctc gcgaggaggc tcggcatccc ggaggacgcg
      961 atggtccgcg tccgggcggg cctctcccac gtcctctcga cggcgctcga cgagggacac
     1021 tgcgggctgc cgcgcgagac gctcatcccg cgcgctgccg agctgctcgg ccggccggaa
     1081 acccaggtcg ccgaagcgct ggagctggag ctcgccgaga gacgcctcgt ccccgaggag
     1141 tcgggcggcc ggccatgcgt cttcctgccc gcgctccacg ccgccgaacg ggccgtggcc
     1201 gagaggctcg tgaggctcgc cgagggcgcg ctgccctgga agcggatcga cccggagcac
     1261 gcgataccct gggtggaggc gagggcctcg atcacgctct cggcgagcca gcggtcggcc
     1321 gtcgccacgt tcctccggtc gaaggtcctg gtcctcacgg gcgggcccgg cgtcgggaag
     1381 acgacgctcg tcgacgctct cctccggatc gtcctcgacg cctccctccg gatcgccctc
     1441 gcggcgccga cggggcgcgc ggcgaaacga ctctccgaga gcacccggtc cgaggcgaag
     1501 acgatccacc ggctgctcga ggtcgacccg tcgaccgggc gattccgccg cgggcgcgac
     1561 aacccgctcg acgcggacct cgtcgtcgtc gacgaggtct cgatggtcga cgtcctgctg
     1621 gcccgggcgc tcctcgaggc ggtcccgaac ggcgcggccc tgctccttgt cggcgactcc
     1681 gatcagctcc cgagcgtcgg accgggccag gtcctgcgcg acctcctctc ctccgacgcc
     1741 gtcccctcgg tccgcctcac cgaggtcttc cgccaggcgg cccagagccg gatcgtcgtc
     1801 ggggcccacc gggtccgcga ggggcacctc cccgacctga cgaacccggc cgggaccgac
     1861 ctcttcttct tcgacgcgcg ggagcccgag gaggcggcgc gccgcgtcgt ggaggtcgtc
     1921 tccgagcgga tcccgcgccg cttcggcctc gacccgcgac gggacgtcca ggtcctcgtc
     1981 ccggtccatc gggggccgct cggagcgcgg gcgctcaacg aggctctcgg gaaggcgctg
     2041 aaccccgacg gcgcgccccg cgtctcccgc ttcgggatgg agctcgcccc gggcgaccgc
     2101 gtcatgcagg tagagaacga ctacgaccgc gaggtctaca acggcgacct cggtctcgtg
     2161 agctgcgtcg accccggcga gggcgagctc cgcgtcgcct tcgacggccg cgaggtgacg
     2221 tacggcttcg acgagctgga cctcctccag ctcgcgtggg cgacgacgat ccacaagtcc
     2281 caggggtcgg agtacccggc ggtcgtcatc ccgctcgcga tgacccacta cgcgatgctc
     2341 gagcggaacc tcctctacac ggcgatgacg cggggccgga agctcgtcgt cctcgtcggc
     2401 gaccggaggg ccatcgccgt cgccgcccgg cgctcctccg cggggagccg cgtcacgcgg
     2461 ctcgcggagc tgctcgctca gaactccagg ttcggctcga tgtaggtcga cggataggga
     2521 gcgtcgagga agacgcgatg tccagccggg gcgacgagga cggcgcggag gagcccggag
     2581 gtgttctctg cgacgaggcg ggcgccggct tcgagtacga cgcggccatc gggtccggaa
     2641 tccagaggca cgccatcgag cagcagccgg acctcggcgg aatccggcgg gcgcagcttc
     2701 gagacgtcca gccgaaaggc cgagccgtcc ttcgccctct ggaacggaaa cgaccacggg
     2761 ctctcgaacc acgggtggag cagccggtgc ggcacctcga cgacccggta cgcaccgccc
     2821 gcgagaatgg tccactcggt cctctcgccg ggctgcaggc gggcattcgg tgaaggcacc
     2881 cagacgaaac gctccagagg gaagaagttc ctcgccacga aggcgcccag ggccttcgtc
     2941 gtcatcgcgg tcgagagcag ccggctgtcg gcgacgacga gggcgacgcg ccgcgactcg
     3001 agctctttca cggtcgggct ctcgagtatc cccgcggcca tgagatcccg agcgagagac
     3061 gggaggaacc aggtttcgag cgcgggacgc cggttgacgg cgaatccaca gccctcgagc
     3121 acgacgctcc cgggccgggt aagccgatcg atcgatcgga ggacgtgatc ctgatgggca
     3181 agcgtctccg cgaggaccgg ccatcggacc gcccgaacgg cggcggtcat tccgacgagc
     3241 gcgaggagcg cagcggcgct cgccacgacg accgcggacc gtcggccgat tcgcatcagc
     3301 gcgtcgaccg tgacggcggc gagaatcgcc aggagccaga acagagtctg gaactggtac
     3361 gggaacggag atcggatcga aaagagaagg atgacctggg ccgcggccag gatggcgacg
     3421 acggtgctca gcctccgtgt ccgggcttgc ctcatcccga ggagcgcgcc cgcggcaccc
     3481 aggacgatca gcgtgacggc acccggacgg tgcaggaagc gagcgagcca gtagacggcg
     3541 ctcgggccgc tgacggtccc ggacgggagc ttctcgtaga ggacgttcag ccggaaacac
     3601 tgttcgaaga gctcgggaag ggcgctcgag atcgacgcga gcccgagcgt gaccaaagcc
     3661 gtgacggcga gtcccgccgc ggcggcgagg gggcggcggg cgatccgcct ccagctgacg
     3721 gcgttcgccg atgccgcgtc cgaagccgca gacgccgcca gcgcgacgag taccgcaggc
     3781 attgcccgct gcgacgccag gcatgccagg cccagcagca tcccgccgaa gaccaaaggg
     3841 gcaggccggg cgcctccgcc gtcgtcctca cgcgtcagcg cgagaagacc ggcgaggagg
     3901 aacaggttca tcgtgacgtc gatccggatc tcgacgacgg aacggtggac ggcgagaaag
     3961 aagcacgaca agccgagaag gctggcggca cggctcacgc ccgcccgcga gagcaggcgc
     4021 gcggccacga ggacggtggc aatcaaaagc gggagctgcg ctccgcgcag gaggaggagg
     4081 ctctcgacgg ccgccgagcg ggccaggccc gccaccggcg cgaggagaac gaagtgaagc
     4141 gggagatggt gctcccagaa atcgcgaaac gggaccaggc cttcgcccac ccagcgagac
     4201 gcgcgaaagt actcgacctc gtcggggttg aagggcgccg cgagaagaag caggaacgga
     4261 ggaagcagcg aaaggacgag ggccgcccag gggaagagac gcatcccgtc cacctgccgg
     4321 agcagcgcat ccgcccccgc gccgacgccc gcttcggcgc cggctcgcct gtcctcccgg
     4381 agcgcttcgt cgggtgccat ccgtcgcctc gtccgatcct acgccagacc cttcgtcgag
     4441 gaacgagcgg accgctggta acctgtgcac gaggagactt cggaatggaa tcgatccgct
     4501 gcgccgacgc cggaggccga cgatgacgtc cggcggggag ctcgtcgcgg aggtcctcct
     4561 ccgccacggc gtccgattcc tcttcacgct ctgcggcggg cacatctccc cgatcctcgc
     4621 cgcggcgaag gcccggggcg tgcgggtcgt cgacacccgg cacgaggcga cggccgtctt
     4681 cgccgccgac gccgtggcgc ggctcaccgg cgtccccggc gtcgccgcgg tcaccgcggg
     4741 ccccggcgtc acgaacgccc tgaccgcgct ccagaacgcg cgcctcgcgc agacgccgct
     4801 cgtccttctc ggcggcgcca cggccaccgt ccttcggggg cgcggcgccc ttcaggacgt
     4861 cgaccagatc gcgctcgcct ccccggccgt caagcgggcg tggcgcgtct cgaagctccg
     4921 cgacctcgtg ccggcgctcg aggaggcgtt cgccgtggcc cgcgacggcg ttcccggccc
     4981 cgtcttcgtg gaatgccccg tcgacctcct ctaccccgag cgggtcgtcc gcagctggta
     5041 ctccacgaag gcgggggcgc cgaaggggct cgtcgcgaag gcgacgcgct ggtatctcga
     5101 caggcacctc gacgcgctct tcgccggggt cgatggggtc gacgcccgag ggccggcgcc
     5161 cggtcctcct cctccccccg aggccgggag cgtcgggacg gcggcgttcc tcctggggcg
     5221 cagcgaacgt cccgtcgccc tcctcgggag cggcgcgctc ctcgacccgg ccggggccgg
     5281 cgcgctcgcg cgcgcggtcg cggagctcgg cctccccgtc tacctctcgg gtatggcgcg
     5341 cggcctcctc ggggccgcgt cgcccctgca gagccgccat cgccgatcgg aggcgattcg
     5401 cgaagccgac ctcgtcctcc tcgcgggcgt cccgtgcgac ttccggctcg actacggccg
     5461 gcagatccgg ccggacgccg tcgtcatctc ggcaaatcgc gactccgccg cccgggccct
     5521 caaccggcgg ccgacgctcg gcgtcggagg cgacccgggc ctcttcctcc gggctctcgc
     5581 cgacgccgtg ccgggcggct tcccccgccc ggagtgggtc gcgcgcctcc gggagcgcga
     5641 cgccgcacgc gaggcagaga tcctcgccgc gcccgaaggt gccccgggcg cgctcgaccc
     5701 ggtcgcgctc tgccgcagcc tcgaggaggc cctcccggca aagggcgtcg tcgtcgccga
     5761 cggaggggac ttcgtcgcca ccgcctccta cgtcctccgc ccccgggcgc cgctctcctg
     5821 gctcgacccc ggcgtcttcg gcacgctcgg cgtcggcgcc ggcttcgcgc tcggagcggc
     5881 gctctgccgc cccgaggcgt ccgtcctgct cctctggggg gatggctcgg ccgggtacgg
     5941 cctcgtcgag atcgacacgt tcgtccggca ctcgctcggc gtcgtcgccg tgatcgggaa
     6001 cgacgccgcg tggacgcaga tcgcgcgcga gcaggtggag gttctcggcg acgacgtcgg
     6061 gaccgtcctc cgctcgaccg actaccacgc cgcggcccg
//
LOCUS       DAOTEZ010000262         6026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_9414_length_6026_cov_6.271814, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000262 DAOTEZ010000000
VERSION     DAOTEZ010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9414_length_6026_cov_6.271814"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="P5164_13345"
     CDS             <1..704
                     /locus_tag="P5164_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44922.1"
                     /translation="GMRDLNGVTTQRFNRRHGRSGPLFAGRFRAVLVEREAHLLALVR
                     HVLNAPVREGLTSAPKEWPWSSFRATAGLVETPRWLDTDATLEALGGRSGEARRRFSA
                     FVEMAAVGDDDPWSRRRGQVFLGSEAFAREAGLKAKRRSSRRTPGGPTTGSVATAFEA
                     ALGVTRRELNEAPRKRLAERALLAWALRCRARAPLARIGSALDVGAAQASVLVRRGAA
                     LAERDPSLSAIVEGL"
     gene            701..1237
                     /locus_tag="P5164_13350"
     CDS             701..1237
                     /locus_tag="P5164_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015204796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HRY44923.1"
                     /translation="MTGREHGPELVVVRGDLTRERVDAIVNAANPSLLGGGGVDGAIH
                     AAAGPGLLAECAGIGGCPPGEARMTGGHRLPARHVLHTVGPVWRGGGAGEDETLARCY
                     RASLRLAEEAGLESVAFPSIATGIYGFPIERASRIAVREIRAFLAGASRVRLVKVVTF
                     HEADHAAYLAAIDGTTSG"
     gene            1234..1923
                     /locus_tag="P5164_13355"
     CDS             1234..1923
                     /locus_tag="P5164_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018887102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HRY44924.1"
                     /translation="MSASARPAVLVAGASGLVGAALLRRLLADAAGSSVVAVVRRPLE
                     LVSPRLVVRVADFGRLDGVDLPPAATAFCALGTTMAKAGSREAFRAVDLDAVVAFARA
                     ARRAGATRFLAVSALGADPGSRVFYNRVKGEAEEALRAVGFEGLVLVRPSLLLGERSE
                     ARPAERAAGVVTRLVPWGLAGPLSRWRPVPAGAVAAALVALAGLPFRGVRVVENDEIH
                     RLASGTSSRVR"
     gene            1934..2695
                     /locus_tag="P5164_13360"
     CDS             1934..2695
                     /locus_tag="P5164_13360"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HRY44925.1"
                     /translation="MSSDTPGFPPAPPLPPPPMPPPPAGGGWQTPPPGGPAPAGLPWE
                     HRSELGFLNAFVETIKLFVTNPAEAWRRTKEKGDFAEPLIFAILVSWIGIAVSTLWSG
                     LFGNAWVAFLPADMKEKMAGAFATNTAALLIQVGLAPIFVLIGLFIGAAIYHLAFLIV
                     GATKDSASGFEGTFRVVSYASVSQLANVIPFVGGFAAVVWNLILVVLGVVRIHRTTQG
                     KAIAGVLIPLLLCCVCIILVVALVVGGLAAAFANR"
     gene            2709..3134
                     /locus_tag="P5164_13365"
     CDS             2709..3134
                     /locus_tag="P5164_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HRY44926.1"
                     /translation="MASGATGAGAGRPPAVQLGLLWGAVAVSAAALAASFRGSVETAL
                     ALLPPCPFKAVTALPCPACGSGRASAALAALDLPAAFASNPLFTLGAFAFLVGGLVAA
                     AFALAGRGVPEPRTLPVSVRAGVVAAVAANWLWLLLDGR"
     gene            3142..3600
                     /locus_tag="P5164_13370"
     CDS             3142..3600
                     /locus_tag="P5164_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44927.1"
                     /translation="MPRAAADPAADFRTLFDALFTFVARHPRAGPKMADLGLSGRGVF
                     PDLKLVLNVAPAGMKLRARGVHVTWSWGAAPKGFAAQVLVSCPSAVANRLCQGKENPA
                     LALDRGDLSLSPLDPSVNERRLLDLLPVLVPLRSEVPGVLRKAGLERLLL"
     gene            complement(3493..4485)
                     /locus_tag="P5164_13375"
     CDS             complement(3493..4485)
                     /locus_tag="P5164_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44928.1"
                     /translation="MEALTTRFATRLLIGTAALLGAAVAVSAPPRLVVEAPPSLAAYA
                     REVERLDPVAFLPAMELTGLFDPGDVRVVVTADGSALATRAPSWAVAYAVPAEGTVVL
                     IPTRTPAWPDDGLPDVVRHEVAHVLAFRAAGGRHVPRWFDEGLAMAAGRPRVIEDDLR
                     EALAVLTGPRLRFAEVDALFGGGRSDANRAYALSSAFFGDLLARHGSAWPKRVLRRMA
                     AGEPFEEAFRGATGVPFRDESGAFWKSRRSVERWLPIVTSTAAVWTAIVLLTLWAFRV
                     ARGRQRRRLAEWEAAERAALQEQTLEPGLPEDAGDLGPEGNEDREEIQKPPLVH"
     gene            complement(4473..5348)
                     /locus_tag="P5164_13380"
     CDS             complement(4473..5348)
                     /locus_tag="P5164_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY44929.1"
                     /translation="MKILIADAFDDSLPGRLAPFGEVTQDAAKAAEADVLLVRSKTKV
                     TKEYLEGAPNLKLIIRGGVGLDNIDVQAAREKGIEVKNTPRASSIAVAELAMALMLTV
                     PNRIIEGHNGIKEGKFLKKELKRTELYGKTLGLLGAGLIGTEVARRAIAFGMRVVAHD
                     HYISNHALADMVEDDDELFALSDYISIHLPLTPETKGMLDRNAFAKMKDGVILINTAR
                     GKVIVEEDLVEALKSGKVRAYATDVYYSDPPDPSCPLLTAPNVVLMPHVGGSSKENLL
                     RIGDNVVDILKKWKR"
     assembly_gap    5689..5788
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5789..>6026)
                     /locus_tag="P5164_13385"
     CDS             complement(<5789..>6026)
                     /locus_tag="P5164_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018307517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HRY44930.1"
                     /translation="LTFVFSTILGWSYYGEKAAEYLFGPKVVRPYRLLWVVAVLVGSV
                     ATLPVVWSFADIANGLMAIPNLISLLALSGVLAA"
BASE COUNT          710 a         2133 c         2197 g          886 t
ORIGIN      
        1 gcgggatgcg cgacctgaac ggcgtcacga cgcagcgctt caaccgccgg cacggccggt
       61 cggggcccct cttcgccggg cgcttccgcg ccgtcctcgt cgagcgagag gctcacctcc
      121 tcgcgctcgt gcgccacgtc ctcaacgctc cggtccggga aggcctgacg agcgctccga
      181 aggaatggcc ctggtcgagc ttccgggcaa cggccggact cgtcgagacg ccgaggtggc
      241 tcgacacgga cgcgacgctc gaggccctcg gcgggcggtc cggcgaggcg cggcgacgct
      301 tctcggcctt cgtcgagatg gccgcggtcg gcgacgacga tccctggagc cggcgaaggg
      361 gacaggtgtt cctcggctcc gaggcgttcg cgcgcgaggc gggcctgaag gcgaagaggc
      421 ggtcgagccg acggacgccg ggcggaccga cgaccggatc ggtcgcgacc gcgttcgagg
      481 cggccctcgg cgtcacgcgg cgggagctga acgaagcgcc gcgaaagagg ctcgccgagc
      541 gggccctcct cgcctgggcg ctccgctgcc gggcgcgcgc ccctctcgct cggatcgggt
      601 cggccctcga cgtcggcgcc gcgcaggcgt cggtcctcgt gcggcgcggg gcggccctcg
      661 cggagcggga cccgtcgctt tctgcgatcg tggaggggct gtgacggggc gggagcacgg
      721 gccggagctc gtcgtcgtcc gcggggacct cacgcgcgag cgggtcgacg cgatcgtgaa
      781 cgccgccaac ccgtcgctcc tcggcggcgg cggcgtcgac ggggcgatcc acgcggccgc
      841 gggaccgggc ctcctcgcgg agtgcgccgg gatcggaggc tgccctcccg gcgaagcgcg
      901 gatgaccgga gggcaccggc tgccggcccg gcacgtcctc cacaccgtcg gaccggtctg
      961 gcgcggcggg ggcgcggggg aggacgagac gctggcgcgc tgctaccgcg cctcgctccg
     1021 gctcgccgag gaggccggcc tcgagagcgt ggccttcccc tcgattgcga ccgggatcta
     1081 cggattcccg atcgagcggg cgagccggat cgcggtccgc gagatccggg ccttcctcgc
     1141 cggcgcctcg cgagtccggc tcgtgaaggt ggtgacattc cacgaagcgg atcacgcggc
     1201 ttatctcgct gccatcgacg ggacgacgtc gggatgagcg cttcggcccg ccccgccgtc
     1261 ctcgtcgccg gggcgagcgg cctcgtcggc gccgcgctcc tgcgccgcct cctcgcggac
     1321 gcggccgggt cgagcgtcgt ggccgtcgtc cgccgtcccc tggagctcgt ctcccctcgc
     1381 ctcgtcgtgc gcgtcgccga cttcggacgc ctcgacggcg tcgacctccc gcccgcggcg
     1441 acggcgttct gcgcgctcgg gacgacgatg gcgaaggcgg gctcgcggga ggcgttccgc
     1501 gcggtcgacc tcgacgccgt cgtcgcgttt gcgcgcgcgg cccgccgggc gggagcgacg
     1561 cgcttcctcg ccgtcagcgc gctcggcgcc gacccgggct cccgcgtctt ctacaaccgg
     1621 gtgaaggggg aggcggagga ggcgctccgc gccgtcggct tcgaggggct cgttctcgtc
     1681 cggccgtcgc tcctcctcgg ggagcggtcg gaggcccggc cggcggagcg agcggccggg
     1741 gtcgtcacac gtctcgtccc gtggggcctc gccggcccgc tctcgaggtg gcgccccgtt
     1801 ccggccggcg cggtcgcggc ggccctcgtg gcgctcgccg ggctgccgtt ccgcggcgtc
     1861 cgcgtcgtcg agaacgacga gatccatcgg ctggcctccg gaacctcctc ccgcgttcga
     1921 tagaatcgcg cgcatgtcga gcgacactcc gggcttccct ccggccccgc ccctgcctcc
     1981 tccgcccatg ccgcccccgc ccgcgggagg aggctggcag acgcccccgc ccggcggccc
     2041 ggcgccggcc gggctcccct gggagcaccg ctccgagctc gggttcctga acgccttcgt
     2101 cgagacgatc aagctgttcg tcacgaaccc ggccgaagcc tggcggcgga cgaaggagaa
     2161 gggcgacttc gccgagccgc tcattttcgc gatcctcgtg tcgtggatcg gcattgccgt
     2221 ctccactctc tggagcggcc tcttcggcaa cgcgtgggtc gcattcctcc cggccgacat
     2281 gaaggagaag atggccgggg cgttcgcgac gaacacggcg gccctgctga tccaggtggg
     2341 tctcgcgccg atcttcgtcc tgatcgggct cttcatcggc gcggcgatct accacctcgc
     2401 cttcctgatc gtcggcgcca cgaaggactc ggcctcggga ttcgagggga cgttccgcgt
     2461 ggtctcctac gcgtccgtct cgcagctcgc gaacgtcatc ccgttcgtcg ggggattcgc
     2521 ggccgtcgtc tggaacctga tcctcgtcgt gctgggcgtc gtccggatac acaggacgac
     2581 gcagggcaag gcgatcgccg gcgtcctgat cccgctgctt ctctgctgcg tctgcatcat
     2641 cctcgtcgtc gcgctcgtcg tcggcgggct cgcggcggcc ttcgcgaacc gctgagccgg
     2701 tgacggacgt ggcgtccggg gcgaccggag ccggagccgg ccgtcccccc gccgtccagc
     2761 tcgggctcct ctggggtgcg gtcgccgtct cggcggccgc cctcgccgcc tcgttccgcg
     2821 ggtccgtcga gacggcgctg gcgcttctcc cgccgtgccc gttcaaggcc gtcaccgccc
     2881 ttccatgccc cgcctgcggg agcggccgtg cctcggcggc gctcgccgcg ctcgacctgc
     2941 ccgcggcgtt cgcgtcgaac ccgctcttca cgctcggcgc gttcgcgttc ctcgtcggcg
     3001 gcctcgtcgc ggccgcattc gcgctcgcgg gaagaggcgt tcccgagccg cgaacgcttc
     3061 cggtttccgt ccgggccggc gtcgtcgccg ccgtcgccgc gaactggctc tggctcctcc
     3121 tggatggaag gtgacggacc gatgccccgt gctgctgcag accccgccgc cgacttccgc
     3181 acgctcttcg acgcgctctt caccttcgtc gcccggcacc ctcgggccgg accgaagatg
     3241 gcggacctcg gcctctccgg ccgcggcgtc ttccccgacc tgaagctcgt cctgaacgtg
     3301 gcgccggcgg gaatgaagct ccgcgcccgt ggcgtgcacg tcacgtggag ctggggggcg
     3361 gcgccgaagg ggttcgcggc gcaggtcctc gtctcctgcc cgtcggcggt cgcgaaccgg
     3421 ctctgccagg ggaaggagaa cccggccctc gccctcgacc ggggagacct gtcgctctcg
     3481 cccctcgacc cgtcagtgaa cgagcggagg cttctggatc tcctcccggt cctcgttccc
     3541 ctccggtccg aggtccccgg cgtcctccgg aaggccgggc tcgagcgtct gctcctgtag
     3601 cgccgcgcgc tcggccgcct cccactccgc gaggcgcctg cgctgccgtc cccgcgcgac
     3661 gcggaaggcc cagagcgtga ggaggacgat cgcggtccag acggccgccg tcgaggtcac
     3721 gatcgggagc cagcgctcga cgctccgccg ggacttccag aacgcgccgg actcgtcgcg
     3781 gaagggaact cccgtcgcgc cccggaacgc ctcctcgaac ggctcgcccg cagccattcg
     3841 gcggaggacc cgcttcggcc aggccgagcc gtggcgggcg aggaggtccc cgaagaacgc
     3901 ggaggagagg gcgtaggcgc ggttcgcgtc gctccgccct ccgccgaaga gggcgtccac
     3961 ctccgcgaag cggaggcggg ggccggtcag gaccgcgagc gcctcgcgga ggtcgtcctc
     4021 gatcacgcgc gggcgccccg ccgccatcgc gagcccctcg tcgaaccagc gcgggacgtg
     4081 ccggccgccc gcggcgcgga aggcgaggac gtgcgccacc tcgtgccgga cgacgtcggg
     4141 gaggccgtcg tccggccagg cgggcgtccg cgtcgggatc agcacgaccg tcccctcggc
     4201 cgggacggcg tacgcgacgg cccacgacgg ggcacgcgtc gcgagggcgc tcccatcggc
     4261 ggtcacgacg acccggacgt cgcccgggtc gaagaggccg gtcagctcca tcgccggcag
     4321 gaaagccacg gggtcgagcc gctccacctc gcgggcgtaa gcggcgaggg aggggggcgc
     4381 ctcgacgacg aggcggggcg gggccgaaac ggcgacggcg gccccgagga gggccgccgt
     4441 cccgatcagg agccttgtcg cgaagcgcgt cgtcagcgct tccacttctt caggatgtcg
     4501 acgacgttgt cgccgatccg gaggaggttc tccttcgagg agccgccgac gtgcggcatc
     4561 aggacgacgt tcggggcggt gaggagcggg caggacgggt cgggcgggtc gctgtagtag
     4621 acgtccgtcg cgtaagcgcg gaccttgccg ctcttgagcg cttcgacgag gtcctcctcg
     4681 acgatgacct tgccgcgcgc cgtgttgatg aggatgacgc cgtccttcat cttcgcgaag
     4741 gcgttcctgt cgagcatccc cttcgtctcg ggggtgagcg gaaggtggat cgagatgtag
     4801 tcggagaggg cgaagagctc gtcgtcgtcc tcgaccatgt cggcgagggc gtggttcgag
     4861 atgtagtggt cgtgcgcgac gacccgcatc ccgaacgcga tcgcgcggcg ggcgacctcg
     4921 gtcccgatga ggccggcgcc gaggaggccg agcgtcttgc cgtagagctc ggtccgcttc
     4981 agctccttct tcaggaactt cccttccttg atgccgttgt gcccctcgat gatccggttc
     5041 gggaccgtca gcatcagggc catcgcgagc tcggcgacgg cgatggacga ggcgcgcggg
     5101 gtgttcttga cctcgatccc cttctcgcgc gcggcctgaa cgtcgatgtt gtcgaggccg
     5161 acgccgcctc ggatgatcag cttcaggttc ggtgcgccct cgaggtactc cttcgtgacc
     5221 ttcgtcttgg agcggacgag aaggacgtcg gcttcggcgg ccttcgccgc gtcctgcgtc
     5281 acctctccga acggcgcgag acggccgggg agggagtcgt cgaaggcgtc ggcgatcagg
     5341 atcttcatct cttcctcctg gtggatctgg gcgccggagc gcccagaggc cattctagga
     5401 cggaagggtc tcgacgacgg cggcggggca ggcggtcggg gccgctcgga ggaaattggc
     5461 cgggccgctc cccgggtcac tcctccgtgg cggcggtgtc gatcatcccg ggtgcgaggt
     5521 acttgcgggt ctcggcggcg aggacgccgc tcagcgcgag gagcgagatg aggttcggga
     5581 tctggagccg ttgcgggctc gcggggctcg cccgcgacgg ctccgtccga acctcactcc
     5641 tccgtggcgg cggtgtcgat catcccgggt gcgaggtact tgcgggtcnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnntc ggcggccagg acgccgctca gcgcgaggag
     5821 cgagatgagg ttcgggatcg ccatgaggcc gttcgcgatg tcggcgaagg accagacgac
     5881 ggggagggtc gcgacggagc cgacgaggac ggcgacgacc cagaggaggc ggtaggggcg
     5941 gacgaccttc gggccgaaga ggtactccgc ggccttctcg ccgtagtacg accagccgag
     6001 gatcgtcgag aagacgaacg tcagga
//
LOCUS       DAOTEZ010000263         6001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_9449_length_6001_cov_7.137908, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000263 DAOTEZ010000000
VERSION     DAOTEZ010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9449_length_6001_cov_7.137908"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..2151)
                     /locus_tag="P5164_13390"
     CDS             complement(160..2151)
                     /locus_tag="P5164_13390"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HRY44931.1"
                     /translation="MHEALRLLLTNAVLAGFLALAAWVVSRFTKRQAVVHGLFLLALL
                     KLVTPPLVELPLLPAGGAVPEATEAASVASEVRTARAAPAPSAGPRARAGEAAGAVAA
                     PEAMRSRSARGALLVEPPTDAGHVAPPGRTSLAGRLALPALVGLGALAVAVLAARRFL
                     RFRRLAEAAEPAPPALVSRAAEIGAALGVRRAPAVYLLAARVPPMLWPASPRPKLLLP
                     RDLLPELDGDELDALLAHELAHVRRRDHWVRFLEIGATALFWWYPVAWWSRCELRRAE
                     ERCCDEWVLRALPDSARAYANGILKSLTFLAEGPVALPETASGAGPIRDLESRLKEIL
                     MTSPRPRLSRPIRLVLGCLAVGALAFFPTVAPPAAAEAAALAGLPDEVPPPPPPAHAS
                     PVAAPAPPAAVAPRSPVAAPAPAPAPPASYAAPHAAPLAAPRLAGHPAPAPDVHPVLA
                     GEELDPELERERRALDAQRRQLDLSRLDLERRALELEARADRQEQAAEVARLRAEGDA
                     AGAARAEKRGALRARQADVERRRLDLHLRQLKLEDESERSAEAVGAERDAEARAREAA
                     RAEAELEKQQQALEAESEKLEREARALEAEARVHALRSAADDYTRALAEQVEELRGAL
                     PEAGAEKAAMERDLARLESALAALRGSRPAKEAPSATVR"
     gene            complement(2156..2542)
                     /locus_tag="P5164_13395"
     CDS             complement(2156..2542)
                     /locus_tag="P5164_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HRY44932.1"
                     /translation="MARTPRDVTDAELEVLRALWDLPEATIRALADRVYPNGGASEYA
                     TVQKLLERLEDKGHVAHRSEGRQNVYTARLGREDLVALRLRETADSLCGGSFTPLLTH
                     LVSGARLSARELRDLRRLVDSLGERR"
     gene            2944..4401
                     /locus_tag="P5164_13400"
     CDS             2944..4401
                     /locus_tag="P5164_13400"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRY44933.1"
                     /translation="MVFSSHVFLFFFLPVVLACNYLLPFRSLSAVLTVLSYLFYGWSN
                     PAWVLLMLAGSYIDFFCGLGLVHFGGGSLAGKDLPALPKGGPRTRGQRICLAASLLSN
                     IGILAFFKYYDFGVVNLEAAARTAGLGSIGLPVLNVALPVGVSFYTFQSMSYAIDVYR
                     GVARPLKNPVDFQLFVSLFPQLIAGPIVRYQTVAEQIRHRTFNLDKLTRGVAFFSLGL
                     GKKILLANPMGHVADAAFNAGTLPWHDAWYGVVAYAFQIYFDFSGYSDMAVGLGLMLG
                     FAFMKNFDDPYLAESITDFWRRWHISLSTWLRDYLYIPLGGNRSGEGRTYANLMTVML
                     VGGLWHGASWNFVIWGAIQGIFLAAERLQGEDSAWRRLPAPFRIAATFLVTCLAWVFF
                     RAETLPQAATYLRSLAGLVEVPAGALAVAASIYTPYHVAIAVACAVVVWTLPQVWDFT
                     RRLTPLKAAACVAVLLASIVVLWTQTTNPFLYFQF"
     gene            4417..>6001
                     /locus_tag="P5164_13405"
     CDS             4417..>6001
                     /locus_tag="P5164_13405"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44934.1"
                     /translation="MAKREKSFSEADSLQDLLGTRVGRPSAWLLTAPFLLVLFAVPLL
                     QLVRPGTKDAAEKPSAPRSSRSTGTFVSRLLSKIPNAEALRAIESRLENESLLRGAVQ
                     RRAQSFLTGFFRAGNSKVVVGSDGWLYYQPGLDFVAGPAITDPDALRRRRKKMVDREL
                     ETEPSPDPRPALFRLHADLAARGVRLVVFPVPDKVSMEPQELGPRFAGAAPDFRPSNR
                     GSATFLDDLRKEGIEVFTLDAASVGEPGERYLRADTHWTPPLMERAARDLAALLRARG
                     SLSPAIEPAPWRVREESRTGGGDLLGMLRLPPDQAIFAPQSVVARVVTNADGSAWEPS
                     AGSEVLLLGDSFTNVYSDPSLGWGSGAGFAEHLSLALGLPIDVVAINGGAAAGARAEL
                     AGPGGLERLENARVVVYEFADRDLTTENWKVLPLPAPREPRPAVAPSAAEAAPAGPFA
                     PAAAPKQPAPAERPRTEPAPTAAPRPTPARAEPVVLHAKVLQVSPVPEPYSAPYADCL
                     STVRLEVVRVESGAWADTHA"
BASE COUNT          740 a         2327 c         2103 g          831 t
ORIGIN      
        1 tcggctccgt cccctgcaag gcctacgaga actggatcta cagcctctcg ggaattacgg
       61 tctggggcgg ctgccgggcg gagttcgagt tcgacgcccg atgagcgagg gagcgcctcc
      121 gcccgcggcg gcggcgctcc ttctccattc cgtcgctctt caccgtaccg tggcgctggg
      181 agcctccttc gcgggccgcg acccgcgcag cgccgcgagc gccgactcca gccgcgcgag
      241 atcgcgctcc atcgccgcct tctcggctcc cgcctccgga agcgcgccgc gcagctcctc
      301 gacctgctcc gcgagggccc gcgtgtagtc gtccgcggcg ctccggagcg cgtggacgcg
      361 cgcctcggcc tcgagcgccc gcgcctcgcg ctcgagcttc tcggactccg cctcgagggc
      421 ctgctgctgc ttctcgagct ccgcctcggc gcgtgcggcc tcgcgggccc gcgcctcggc
      481 gtccctttcc gcgcccacgg cctccgccga gcgctccgat tcgtcctcga gcttcagctg
      541 gcgaaggtgc aggtcgaggc gtcgcctctc gacgtccgcc tggcgggcgc gaagcgcgcc
      601 gcgcttctcc gcgcgcgccg ccccggccgc gtctccctcg gcccggaggc gcgcgacctc
      661 ggcggcctgc tcctgccggt ccgcccgggc ctcgagctcg agggcgcgcc gctcgaggtc
      721 gagccgggag aggtcgagct gccgcctctg agcgtcgagc gcacgccgct ccctctcgag
      781 ctccgggtcg agctcctccc cggcgagcac cgggtggacg tcgggcgccg gcgcgggatg
      841 cccggcgagc cgcggtgccg ccagcggcgc tgcgtggggt gccgcgtagg acgcgggcgg
      901 agccggggcc ggcgccggag ccgcgaccgg cgagcggggg gcgacggcgg ccggaggcgc
      961 gggggccgcg acgggcgacg cgtgcgccgg cgggggcggg ggcgggacct cgtcgggaag
     1021 gccggccagc gccgcggcct ccgccgcggc cggcggcgcg accgtcggga agaacgcgag
     1081 cgcgccgacc gcgagacagc cgaggacgag ccggatcggg cgggagagcc ggggtcgcgg
     1141 tgaggtcatg aggatctcct tcagccggga ctcgaggtcc cgtatcggtc cggcgccgga
     1201 cgcggtctcc ggaagggcga cgggcccctc cgcgaggaac gtcaggctct tcaggattcc
     1261 gttcgcgtag gcacgcgccg agtccggaag cgcgcgaagg acccactcgt cgcagcagcg
     1321 ttcctcggcg cggcggagct cgcaccggct ccaccaggcg accgggtacc accagaacag
     1381 cgccgtcgct ccgatctcca ggaaccgcac ccagtggtct cgccgcctca cgtgggcgag
     1441 ctcgtgggcg aggagggcgt cgagctcgtc tccgtcgagc tccgggagga ggtcgcgcgg
     1501 caggagcagc ttcggacgcg gcgaggccgg ccagagcatc ggcgggaccc gggccgcgag
     1561 caggtagacc gcaggagcgc ggcgcacgcc gagggccgcg ccgatctcgg cggcgcggga
     1621 cacgagcgcg ggcggagccg gctccgccgc ctccgcgagg cgccgaaacc gaaggaagcg
     1681 ccgcgcggcg agcacggcga cggcgagcgc gccgaggccg acgagcgccg gcagggcgag
     1741 gcgtccggcg agcgacgttc gccccggagg agcgacgtgg ccggcgtcgg tcggcggctc
     1801 gaccaggagc gctcctcgcg ccgatcgcga ccgcatggcc tcgggagcgg cgacggctcc
     1861 cgccgcttcg ccggcccgcg cccgcgggcc ggcggacggc gcgggagcgg cgcgcgccgt
     1921 cctcacctcg gaggcgacgg aagccgcttc cgtcgcctcc gggacggccc cgcccgccgg
     1981 aaggagcggc agctccacga gcggcggcgt cacgagcttc agcagggcga ggaggaagag
     2041 cccgtgcacg accgcctgcc gcttcgtgaa gcgcgagacc acccaggccg cgagggcgag
     2101 gaagcccgcg aggaccgcgt tcgtcaggag gagacggaga gcctcgtgca cgccgtcacc
     2161 tccgctcgcc gagcgagtcc accaggcgcc gcaggtcgcg cagctcccgg gcggagagcc
     2221 gcgctccgct gacgaggtgc gtcaggagcg gcgtgaacga gccgccgcag agcgagtccg
     2281 ccgtctcgcg cagccggagc gcgacgaggt cctcgcggcc gaggcgggcc gtgtagacgt
     2341 tctgccgccc ttcggagcgg tgagccacgt gccccttgtc ctcaagccgc tcgaggagct
     2401 tctggacggt ggcgtactcg gaggcgccgc cgttcgggta gacccggtcc gccagcgccc
     2461 ggatcgtcgc ctccggaagg tcccagagcg cgcgcagcac ctcgagctcc gcgtcggtca
     2521 cgtcccgggg ggttcgcgcc atcggcccct cctgtcgtcc cgtcttctca gacgttacgt
     2581 ctgagaatct gagacgtcac gtctgagttg tcaagggcga agggcggaat cggcgcagac
     2641 ctcccttcgc gtccgtcgcc gtcccgccgt cccgtcccct ccgccggccg atgcggttct
     2701 gtataatcgg cccttcgacg aagcagcgga gcccgtcggg cgaggagaga agagctgcgg
     2761 gacgagacga caggcgcggc gacgcggacg cggacggccc cgctcctggg cgcggccccg
     2821 tccgctcccg ccgggaacca cggacgtccg caggggaccg ggacgacctc gaggaggccg
     2881 cgggccgggg cctgaggccc ttcgctccgc gatccccgcc cgagggcccg agagcctccc
     2941 aagatggtct tcagctcgca cgtcttcctc ttcttcttcc tgccggtcgt cctggcctgc
     3001 aactacctcc tcccgtttcg gtcgctctcg gccgtcctga cggtcctgag ctacctcttc
     3061 tacggctggt cgaaccccgc gtgggtcctc ctgatgctcg cgggctctta catcgacttc
     3121 ttctgcgggc tcgggctcgt ccatttcgga ggaggctccc tcgccgggaa ggacctcccc
     3181 gcgctcccga agggagggcc gcggacccgc gggcagagga tctgcctcgc cgcgtcgctc
     3241 ctctccaaca tcgggatcct cgcgttcttc aagtactacg acttcggcgt cgtgaacctc
     3301 gaggccgccg cgcgcaccgc gggcctcggc tcgatcggcc tgccggtcct caacgtcgcc
     3361 ctcccggtcg gcgtctcctt ctacacgttc cagtcgatga gctacgcgat cgacgtctac
     3421 cgcggcgtgg cgcggcccct gaagaacccc gtcgacttcc agctcttcgt gtccctcttc
     3481 ccgcagctca tcgcggggcc gatcgtccgc taccagaccg tcgccgagca gatccggcat
     3541 cgcacgttca acctcgacaa gctcacccgc ggcgtcgcgt tcttctccct cggcctcggc
     3601 aagaagatcc tcctcgcaaa ccccatgggg cacgtggccg acgcggcgtt caacgccggg
     3661 acgctcccct ggcacgacgc ctggtacggc gtcgtcgcct acgcgttcca gatctacttc
     3721 gacttctcgg gctactccga catggccgtg ggcctcggcc tgatgctcgg cttcgcgttc
     3781 atgaagaact tcgacgaccc gtacctcgcc gagagcatca cggacttctg gaggcgctgg
     3841 cacatctcgc tctcgacctg gctccgcgac tacctctaca tcccgctcgg cgggaaccgc
     3901 tccggcgagg ggcggaccta cgcgaacctg atgaccgtca tgctcgtcgg ggggctctgg
     3961 cacggcgcct cctggaactt cgtgatctgg ggcgcgatcc agggcatctt cctcgccgcc
     4021 gagcggctcc agggggaaga ctccgcgtgg cggcgcctgc cggcgccgtt ccggatcgcc
     4081 gccacgttcc tcgtcacctg cctcgcctgg gtcttcttcc gggcggagac gctcccgcag
     4141 gccgcgacgt acctccgctc gctcgcgggc ctcgtggagg tgccggccgg cgcgctcgcg
     4201 gtcgccgcct cgatctacac gccgtaccac gtcgcgatcg ccgtcgcctg cgccgtcgtc
     4261 gtctggaccc tcccgcaggt ctgggacttc acccggcgtc tgacgccgct gaaggccgcc
     4321 gcctgcgtcg ccgtcctcct cgcgagcatc gtcgtcctct ggacccagac gacgaacccg
     4381 ttcctctact tccagttctg agaggacgac gggacgatgg cgaagagaga gaaatcgttc
     4441 tccgaggccg acagcctcca ggacctcctc gggacgcgcg tcggccgccc ttccgcgtgg
     4501 ctcctcacgg ctccgttcct cctcgtcctt ttcgcggtcc ccctcctcca gctcgtccgc
     4561 ccggggacga aagacgcagc agagaagccc tcggccccgc gctcctcgcg gtcgacaggc
     4621 acgttcgtct cccgcctcct ctcgaagatc ccgaacgcgg aggcgctgcg ggcgatcgag
     4681 tcgcgcctcg agaacgagag cctccttcgc ggcgccgtcc agaggagggc gcagtccttc
     4741 ctcacgggct tcttccgggc cggcaacagc aaggtcgtcg tcggttccga cggctggctc
     4801 tactaccagc ccggcctcga cttcgtcgcc ggaccagcca tcaccgatcc cgacgcgctc
     4861 cggcggcgcc ggaagaagat ggtcgaccgc gagctcgaga ccgagccgag ccccgacccg
     4921 cgcccggccc tcttccgcct ccacgccgac ctcgcggcgc gcggcgtccg cctcgtcgtc
     4981 ttcccggtgc ccgacaaggt ctcgatggag ccgcaagagc tcgggccgag attcgcgggg
     5041 gcagctcccg acttccgccc gtcgaaccgg ggctccgcga cgttcctcga cgacctgcgg
     5101 aaggagggga tcgaggtctt cacgctcgac gcggcctccg tgggggagcc gggcgagcgt
     5161 tacctgcggg ccgacacgca ctggacgccg cccctcatgg agcgcgccgc acgggacctc
     5221 gcggctctcc tccgcgcccg cgggtccctt tcgccggcga tcgagcccgc gccctggcgg
     5281 gtccgcgagg agagccgcac gggtggcggc gacctcctcg gaatgctccg gctgccgccg
     5341 gaccaggcga tcttcgcccc gcagagcgtc gtcgcgcgcg tcgtgacgaa cgcggacggg
     5401 agcgcgtggg agccctccgc gggctccgag gtcctcctcc tcggcgacag cttcacgaac
     5461 gtctactccg acccgtccct cggctggggc tcgggcgccg gcttcgccga gcacctctcg
     5521 ctcgccctcg gcctgccgat cgacgtcgtg gcgatcaacg ggggcgccgc ggccggcgcg
     5581 cgggccgagc tcgccggccc gggcggcctc gagcggctcg agaacgcgcg cgtcgtcgtc
     5641 tacgagttcg ccgaccgcga cctgacgacg gagaactgga aggtcctccc gctgccggcc
     5701 ccgagggagc cgcgccccgc cgtcgcccca tccgccgccg aggccgcgcc ggccggcccc
     5761 ttcgcgccgg ccgcggcccc gaagcagccg gcgcccgcgg agaggccccg cacggagccg
     5821 gccccgaccg cggcgccccg gccgaccccc gcgcgcgcgg agccggtcgt cctccacgcg
     5881 aaggtccttc aggtctcgcc cgtccccgag ccgtacagcg cgccgtacgc cgactgcctc
     5941 tccacggtcc ggctggaggt cgtgcgcgtc gagtcgggcg cctgggccga cacgcacgcg
     6001 a
//
LOCUS       DAOTEZ010000264         5983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_9492_length_5983_cov_8.267038, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000264 DAOTEZ010000000
VERSION     DAOTEZ010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9492_length_5983_cov_8.267038"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="P5164_13410"
     CDS             <1..307
                     /locus_tag="P5164_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRY44935.1"
                     /translation="CHVQTIYDPEKCVLCSRCVDVCPEYCLAIVPFEELELDEAERSL
                     LEERANGNGLPLSAMVKDDDKCIRCGLCAVRCPTDAMTMERFTITERLVPKTSEVSR"
     gene            304..852
                     /locus_tag="P5164_13415"
     CDS             304..852
                     /locus_tag="P5164_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HRY44936.1"
                     /translation="MTTKTDPPAGATAPPAEPNSRREFLMKAGIGAGVVALVGQAGAS
                     LRSLVPNVSYDAPTTVKLGAPTDFPDGMKFLPDERLFVFREGKTFHAISAVCTHLGCT
                     VRAEALSKVEEASVEGKPLRLTHRFQCPCHGSKYSGDGTNETGPAPKPLAWYQLSVAA
                     DDGQLVVDLAQEVGHDFRLTVA"
     gene            869..2209
                     /locus_tag="P5164_13420"
     CDS             869..2209
                     /locus_tag="P5164_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HRY44937.1"
                     /translation="MSAKTETPAPSAPGVHPKPFWSFRPRSDREAGDAVVSNFLLHWF
                     PAKTYKPSLDWGYSFWLGTASAALFFLLVLSGLPLLLLYVPSVERAYGSVKDIEFVVT
                     FGSWIRAVHRISAHLMVAAVFLHLVRVFLTGAYKNGTGRGQRREWNWVLGVVMIVLTL
                     FLSFTGYLLPWDQLAYWAVTVGTNIASSVPFVGPEVRELLIGGRTIDQATLIRFYVLH
                     VIFLPAGLGALLAYHMWRIRKDGGLAKADRWTLSQEKTEAPATRTKTYSLLGVARGQA
                     PAIRAVTLEARDTTVNSVNDLVRRIAIVVLGTFAVVSILACFIPSPLEEPANPMITPN
                     PAKAPWYFLWLQEIVTDTTLKIGSFTINGAFLGGVILPTILLGLLLVWPWLDRSPAGA
                     AGVWFHASRKRQNLVFLLLVLVVLAFTIVGTFLRGPYWNFYWPWEAWPELPTRI"
     gene            2212..3432
                     /locus_tag="P5164_13425"
     CDS             2212..3432
                     /locus_tag="P5164_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRY44938.1"
                     /translation="MEQRTSSPYPGKDRPVLAVIGVLLVVSTVVFAVTDHRKDWRWYQ
                     AEFRKMVAEKYGAEKAASVPAGMKQIWVKDLGRADRCITCHQGILWKGFETAEHPWKT
                     HPAEPLKIHPVEDYGCTACHGGQGWAVESEKAHGPIHFWEEPVLGTAMGEAYSLQGGD
                     KGALMQMNCNVCHRYDRETKGADLINLGKKLVNEKGCRACHVINGRGGTIGPDLTYVG
                     DKAAEQYDMSRLGGQKTAFAWHVAHLKNPKELVPDTVMPSFGFGTKESQALAALVLSW
                     RRAPVPATYRPGVPRTDPQTPEEIAEEERMKHGPGAWFVSTGCFVCHSVSSLGVKSAA
                     QVGPDLSTAVEDTQSRFGKTVDDFIRNPTGTMSVVLSRQIVLTDAEKEVAIQKLREAF
                     AEHQKQKAAAAAAR"
     gene            3542..5410
                     /gene="nosZ"
                     /locus_tag="P5164_13430"
     CDS             3542..5410
                     /gene="nosZ"
                     /locus_tag="P5164_13430"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HRY44939.1"
                     /translation="MTEKKKSVLTWGVLGAIVVAGVLAGRCAPSGPKGKKAPAAASDI
                     AVAAQKTYVAPGDLDEYYFFASGGHSGQVYVYGVPSMRHLSTIPVFTPYPATGYGFDD
                     ESKAMLNGLTWGDAHHPALSETAGDYDGRWLFINDMNARIARIDLRDFKTKQILGPVA
                     NVSGNHSGAYVTPNTEYAMMGSRFSAPVPKTNAVGIDKYASEYKGILAAIKIDPKSGE
                     MSLGWQIVTPPFNWDLGDAGKKVSDGWMFWTCYNSERATGKLEVTASQADRDYIAAVD
                     WRAAEKAIAEGKGEMLGGVRVLDPKKVPGLMYLMPCGKSPHGVDVSPDGKYVIGSGKL
                     QGVTTAFNFEKVLTAIRNKDFTGDEDGIPVLKYESIKDAEVEVGLGPLHTQFDGDGYA
                     YTSLFVDSAIAKWKLGTWEVVDKIPMSYSIGHLCAAEGDTVSPDGKYLVGLNKLSHGR
                     HLNVGPSQPESSQLVDISEEKMKLLYDAFTEPEPHYAVMVKADKLKPIEVYPKEENKH
                     PLAIWDVKDAGVTRTGTEVLAKVVLVRSTITPELIEVSQGDTVKVALTNIEQTTDELH
                     GWGLLDYNINIVVDPGETKVVTFKADKPGVFAYYCTNFCSALHQEMQGYLVVKPKG"
     gene            5541..>5983
                     /locus_tag="P5164_13435"
     CDS             5541..>5983
                     /locus_tag="P5164_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44940.1"
                     /translation="MRSLLERSNRLLEQPIGLKPRLLLVAATICLLGAYLFPLWNLTM
                     FAPQYQDGLRLDIYSYKFEAGNGGQDLKEINVLNHYIGMRDLEPENFTEFKWIPFVVG
                     IMGLLFLRAAVFGHMAHVLDVLVLYSWFGLFSLWSFGYKMYRYGH"
BASE COUNT          995 a         2176 c         1909 g          903 t
ORIGIN      
        1 ctgccacgtc cagacgatct acgacccgga gaagtgcgtc ctctgcagcc gctgcgtcga
       61 cgtctgcccc gagtactgcc tcgccattgt cccgttcgag gagctcgagc tcgacgaggc
      121 ggagcggtcg ctcctcgagg agcgggcgaa cgggaacggg ctcccgctct cggcgatggt
      181 caaggacgac gacaagtgca tccgctgcgg cctctgcgcc gtccgctgcc ccaccgacgc
      241 gatgacgatg gagcgattca cgatcacgga acgtctcgtt ccgaagacga gcgaggtctc
      301 acgatgacga cgaagacgga ccccccggcc ggcgccaccg cgcctcccgc cgagccgaac
      361 agccgccgcg agttcctgat gaaagccgga atcggcgccg gcgtcgtcgc cctggtcggg
      421 caggcgggcg cctccctgcg ctcgctcgtc ccgaacgtgt cgtacgacgc gccgacgacg
      481 gtgaagctcg gggcgccgac ggacttcccc gacgggatga agttcctccc cgacgagagg
      541 ctcttcgtct tccgcgaggg gaagacgttt cacgcgatct cggccgtctg cacgcacctc
      601 ggctgcaccg tgagggccga ggcgctctcg aaggtcgagg aggcgagcgt cgagggcaag
      661 cccctccgcc tcacgcaccg cttccagtgc ccctgccacg gctcgaagta ctccggcgac
      721 gggacgaacg aaacggggcc ggcgccgaag ccgctcgcct ggtaccagct ttccgtcgcc
      781 gctgacgacg ggcagctcgt cgtcgacctc gcacaggaag tcgggcacga cttccgcctg
      841 acggtcgcct gagcggagcg ggacgagctt gagcgcgaag accgagactc ccgccccctc
      901 cgctcccggc gtccacccga agcccttctg gagcttccgg ccccgctccg accgcgaggc
      961 gggcgacgcc gtcgtctcga acttcctcct ccactggttc cccgcgaaga cctacaagcc
     1021 gtcgctcgac tggggctact cgttctggct cggcacggcc tcggccgccc tcttcttcct
     1081 cctcgtcctc tccggcctcc cgctcctcct cctctacgtc ccctcggtcg agcgggccta
     1141 cggctcggtg aaggacatcg agttcgtcgt cacgttcggc tcctggatcc gggccgtcca
     1201 caggatctcg gcgcacctga tggtggccgc ggtcttcctc cacctcgtga gggtcttcct
     1261 gacgggggcc tacaagaacg ggacggggcg cggtcagcgc cgcgagtgga actgggtcct
     1321 cggcgtcgtc atgatcgtcc tgaccctctt cctctccttc accgggtacc tcctcccctg
     1381 ggaccagctc gcctactggg ccgtcacggt cgggacgaac atcgcgagct ccgtgccgtt
     1441 cgtcggcccc gaggtccgcg agctgctcat cggcgggcgg acgatcgacc aggcgacgct
     1501 gattcgcttc tacgtcctgc acgtcatctt cctgcccgcc ggcctcgggg ccctcctcgc
     1561 gtaccacatg tggcgcatcc gcaaggacgg agggctcgcg aaggccgacc gctggacgct
     1621 ctcgcaggag aagaccgagg ctccggccac gcggacgaag acgtattccc tcctcggcgt
     1681 cgcccgcggc caggccccgg cgatccgcgc cgtgacgctc gaagcgcggg acacgacggt
     1741 gaactccgtc aacgacctcg tccggcggat cgcgatcgtc gtcctcggaa cgttcgccgt
     1801 cgtgagcatc ctggcctgct tcatcccgtc gcccctcgag gagccggcca acccgatgat
     1861 cacgccgaac ccggcgaagg cgccgtggta cttcctctgg ctccaggaga tcgtcaccga
     1921 cacgactctg aagatcgggt cgttcacgat caacggcgcc ttcctcggcg gcgtcatcct
     1981 gccgacgatc ctcctcggcc tcctcctcgt ctggccctgg ctcgaccgga gcccggccgg
     2041 cgcggccggg gtctggttcc acgcctcgag gaagaggcag aacctcgtct tcctcctcct
     2101 cgtcctcgtc gtcctcgcgt tcacgatcgt cgggaccttc ctccggggtc cctactggaa
     2161 cttctattgg ccgtgggaag cctggcccga gctcccgacg aggatctgac gatggagcag
     2221 cgcacgagct cgccctaccc cggcaaggac cgtcccgtcc tcgccgtcat cggggtcctc
     2281 ctcgtcgtct cgacggtcgt cttcgccgtc accgaccacc ggaaggactg gcgctggtac
     2341 caggcggagt tccggaagat ggtcgccgag aagtacggcg ccgagaaggc cgcttccgtg
     2401 ccggccggga tgaagcagat ctgggtgaag gacctcggcc gcgcggaccg gtgcatcacc
     2461 tgccaccagg ggatcctctg gaagggcttc gagacggccg agcacccctg gaagactcac
     2521 cccgccgagc cgctgaagat ccaccccgtc gaggactacg gctgcaccgc ctgtcacggc
     2581 ggccagggct gggccgtcga gagcgagaag gcccacgggc cgatccactt ctgggaggag
     2641 cccgtcctcg ggacggcgat gggcgaggcc tactcgctcc aggggggcga caagggcgcc
     2701 ctgatgcaga tgaactgcaa cgtctgccac cggtacgacc gggagacgaa aggggccgac
     2761 ctcatcaacc tcggcaagaa gctcgtcaac gagaagggct gccgggcctg ccacgtcatc
     2821 aacgggcgcg gcgggacgat cggtcccgac ctgacgtacg tcggcgacaa ggccgccgag
     2881 cagtacgaca tgagccgcct cggcggccag aagaccgcgt tcgcctggca cgtcgcgcac
     2941 ctgaagaacc cgaaggagct cgtccccgac accgtcatgc cgagcttcgg cttcgggacg
     3001 aaggagtccc aggctctcgc ggccctcgtc ctctcctggc gccgcgcgcc cgtcccggcg
     3061 acgtaccgcc ccggcgtccc gcggaccgac ccgcagacgc ccgaggagat cgccgaggag
     3121 gagcggatga agcacggccc cggggcctgg ttcgtctcga ccggctgctt cgtctgccac
     3181 tcggtctcct cgctcggggt gaagagcgcc gcgcaggtcg ggcccgacct ctcgacggcc
     3241 gtcgaggaca cgcagagccg attcggcaag accgtggacg acttcatccg aaacccgacc
     3301 gggacgatgt ccgtcgtcct gtcgcgccag atcgtcctca ccgacgccga gaaggaggtc
     3361 gcgatccaga agctgcgcga agccttcgcc gagcaccaga agcagaaggc cgcggccgcc
     3421 gcggcgcgct gatcgactcc ggagggcgcg cgccgatccc gcgcgtcctc cgttcaccgc
     3481 acctcgaccg attcgcctcc ggcctcgagc cgggggctca cagggagaag gaggggacca
     3541 gatgacggaa aagaagaaga gcgtcctgac gtggggcgtc ctcggggcca tcgtcgtggc
     3601 gggcgtcctg gcgggacgct gcgccccgtc cgggccgaag gggaagaagg cgccggcggc
     3661 ggcctcggac atcgccgtcg cggcacagaa gacctacgtc gccccgggcg acctcgacga
     3721 gtactacttc ttcgcctcgg gcggccactc cgggcaggtg tacgtgtacg gcgtgccgtc
     3781 gatgcggcac ctctccacga tcccggtctt cacgccctac ccggccaccg gctacggctt
     3841 cgacgacgag tcgaaggcga tgctgaacgg cctgacctgg ggcgacgccc accaccccgc
     3901 cctgtccgag acggcgggcg actacgacgg gcgctggctc ttcatcaacg acatgaacgc
     3961 gcgcatcgcc cggatcgacc tgcgcgactt caagacgaag cagatcctcg ggcccgtcgc
     4021 gaacgtctcc gggaaccact ccggcgccta cgtcacgccg aacaccgagt acgcgatgat
     4081 ggggtcgcgc ttctccgcgc ccgtcccgaa gacgaacgcc gtcgggatcg acaagtacgc
     4141 ctccgagtac aaggggatcc tggccgcgat caagatcgac ccgaagagcg gcgagatgtc
     4201 gctcggctgg cagatcgtga cgccgccctt caactgggac ctcggcgacg ccggcaagaa
     4261 ggtctccgac gggtggatgt tctggacctg ctacaactcc gagcgggcga ccggaaagct
     4321 cgaggtgacc gcctcgcagg ccgatcgcga ctacatcgcc gccgtcgact ggagggccgc
     4381 cgagaaggcc atcgccgagg ggaagggcga gatgctgggt ggcgtcaggg tcctcgaccc
     4441 gaagaaggtc cccggcctca tgtacctgat gccgtgcggc aagtcgcccc acggggtcga
     4501 cgtctccccc gacggcaagt acgtcatcgg gtccggaaag ctccagggcg tcacgacggc
     4561 cttcaacttc gagaaggtcc tgacggcgat ccgcaacaag gacttcaccg gcgacgagga
     4621 cggcattccc gtccttaagt acgagtcgat caaggacgcc gaggtcgagg tcggcctcgg
     4681 cccgctccac acgcagttcg acggcgacgg ctacgcctac acctcgcttt tcgtcgactc
     4741 ggcgatcgcg aagtggaagc tcggcacctg ggaggtcgtc gacaagatcc cgatgtccta
     4801 ttcgatcggc cacctctgcg cggccgaggg cgacaccgtg agcccggacg ggaagtacct
     4861 cgtcggcctc aacaagctct cgcacggccg gcacctgaac gtcgggccgt cgcagcccga
     4921 gtcgtcccag ctcgtcgaca tctccgagga gaagatgaag ctcctctacg acgccttcac
     4981 cgagcccgag ccccactacg cggtcatggt gaaggccgac aagctcaagc cgatcgaggt
     5041 ctatcccaag gaggagaaca agcacccgct cgcgatctgg gacgtcaagg acgccggcgt
     5101 cacccgcacc gggaccgagg tcctcgccaa ggtcgtcctc gtccggtcga cgatcacccc
     5161 cgagctcatc gaggtgagcc agggcgacac cgtcaaggtc gccctgacga acatcgagca
     5221 gacgacggac gagctccacg gatggggcct cctcgactac aacatcaaca tcgtcgtcga
     5281 cccgggcgag acgaaggtcg tcacgttcaa ggccgacaag ccgggcgtct tcgcctacta
     5341 ctgcacgaac ttctgctccg cgctccacca ggagatgcag ggctacctcg tcgtcaagcc
     5401 gaagggctga cgcctctccc tccgggcggg gcggcgccgc gaggcgccgc cccgcccccc
     5461 gctcctcgcc ccttcgtccg cggccgtccc cgggggcgcg gcccggaccg cttcccggac
     5521 ccccgaccga gggcaccgaa atgcgatcgc tcctggaaag aagcaaccgt ctcctcgagc
     5581 agccgatcgg cctgaaaccc cggctcctcc tcgtcgccgc gacgatctgc ctcctcgggg
     5641 cgtacctctt cccgctctgg aacctgacga tgttcgcccc gcagtaccag gacggcctcc
     5701 gcctcgacat ctacagctac aagttcgagg cgggaaacgg cgggcaggac ctcaaggaga
     5761 tcaacgtcct gaaccactac atcggcatga gggacctcga gcccgagaac ttcaccgagt
     5821 tcaagtggat ccccttcgtc gtggggatca tgggcctcct gttcctgcgc gccgcggtct
     5881 tcggccacat ggcccacgtg ctcgacgtgc tggtcctcta cagctggttc gggctcttct
     5941 cgctctggtc cttcggctac aagatgtacc gctacgggca cga
//
LOCUS       DAOTEZ010000265         5953 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_9555_length_5953_cov_5.649203, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000265 DAOTEZ010000000
VERSION     DAOTEZ010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5953
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9555_length_5953_cov_5.649203"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="P5164_13440"
     CDS             <1..595
                     /locus_tag="P5164_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44941.1"
                     /translation="SGFDWAALGHHHRLEVVTREDGSPAGAYSGSPTGRGLDETGPRH
                     FLKVTLEEGSPARVETLSADCRKVLDLELDAAGRDGEGLRDAALALLLEAGATPGDLV
                     RLTVVGRPAAATRASQALGDLKGLVAHLALRDRTLGDAEARQDRGTAEGRFALELESR
                     LAATEDPRVRRVLELASVLGREALLGRVPAPPAEEDL"
     gene            598..3510
                     /locus_tag="P5164_13445"
     CDS             598..3510
                     /locus_tag="P5164_13445"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY44942.1"
                     /translation="MERVVVEGFGPLEGLDLAWGDGELLLVLEPNETGKSTLCEAITA
                     ALYGLPKGRSAGGRSRESRRPRSGAPTRLRLDVSAAAGRLSIERDFDEGTLRVVDRDR
                     GEDRTAEFLRAGGRDAVGEKLTGLTEALFRTTAYVGQNVLDGDALDSSLTVELARIAD
                     SGGGEASVVRALRLLEAARARMPDATTGAQVSVETEIVRASRRVEEKRAEVARLAKAR
                     EAAAEAAVRLASRTAERDAVRREAALAEVAVVESERRSLSAELERRTAEQRRRGELAA
                     EARSLEREAALFAPEALAAVDALRSARGSRPEALVAARAAIAAERAATEAEDRERRKR
                     AGGLADLDPEARALLRRHLEASREAESESEAAEKALEAEWQELRRDGLAEDLRRLDGL
                     GAADRDFVVAAEESRQGLELEGVQLDRRFADAGARASIVAGERRVLVRRARALVVAAS
                     LVAPLAVGTLVRRGRLDDPLVLMLFAFGVVLALYGGIAWARGDRYGREEEARFRQEEA
                     AARAEAQETRRRLSELRLRLEQIARGAGFKDSAGLLKAQRKARAADEERQRLVEKRVR
                     LFAAGERLAAAAKGLEAFRGPLGLASGLPTADESRRVLQMLTDLEKADSALAGRREVA
                     EREGERLEAEERELRRLEEELAVLLARLEVPSGLSLPESLLFVEAGRARAARRREILE
                     VELPALAVAPLPDGDEAFEERVRLLAAEVEMRLDVLGAVLSDLSVPAEPEAARRAAEA
                     LRERAARLEDERQAAERELAQRAMEGGERGREAEEQLGALDVARERAVLYRDGLDAAR
                     EVLASAATTTYGDFRAGLARASREILARCRLPYEALEFGEDLSVSVVGRGGRPVTRAE
                     LGASVSTGAREQLHLVARLAALRHLGTGPRGLPLLLDDPLVGADDDRFGAVLEFLLDD
                     VLAERPVLLVSCHRSRHERWLGALSPERAARVRRVRLSGGGRPEPERELTPAPGGE"
     gene            3621..5252
                     /gene="secD"
                     /locus_tag="P5164_13450"
     CDS             3621..5252
                     /gene="secD"
                     /locus_tag="P5164_13450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRY44943.1"
                     /translation="MKRPTGWRLALTLVVLIGSIAAYVYRGREALKKNPTAPPNPSIG
                     DVLKNGLKLGLDLRGGIHLVMQVKAEDALRAEANDAAEHLKTEAGKRGVTLGTVNPAT
                     HEGFTITMGEDVSPAAVSDLVKSALSPTDWASDQSGREMRVTFRDPARIEIEGQTLRQ
                     AVETIRNRVDALGVAEPAIQPQGNDRIVIQLPGVDDPARVKDIIGTTGLLELKIVDLK
                     GGPYSSPEAAAAAYGGTIPANLELVEGNAEDEQARTRTTVYYVLERASAVTGRDLKNA
                     RVGQDKFNQPAVEFFLNAAGAEKFGRVTGDNVGRQLAIVLDKRVQSAPNIKSQINDNG
                     IIEGNFTPERADSLALVLRSGALPAQLVILEERTVGPSLGLDSIKQGILAALVGIALV
                     FIAVVVYYNLAGLNAVLALTMNAIILLGAMAWINATLTLPGIAGFILTIGMAVDANVL
                     IFERIREELDAGKGAKAAIDLGFKKALSAIVDSNLTTIAAAAFLFQFGTGPVKGFAVT
                     LVIGLCASMFTAVFVSHLLFDLLYGSKEKVETISI"
     gene            5261..>5953
                     /locus_tag="P5164_13455"
     CDS             5261..>5953
                     /locus_tag="P5164_13455"
                     /inference="COORDINATES: protein motif:HMM:NF019187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44944.1"
                     /translation="MRLLRNPNFDFLKLQKPFVIGSIVAAVVSAGAIATKGLNWGVDF
                     TGGAQIVYAFQQTPDEDQVRKIVEGAGVPVTSVQRYDKAEKNQILLRVPMEQKEGRDI
                     SAEVTPALVKALSPAGRDEGAFDLNLNGADPLSAKLQQDDPEKMAGRADANPTVEYGR
                     IAQSIIAQRSQAGLFASLDAAAATPGLSPAVASWLKEKTVAGPVTLISAESVGPAVGK
                     DLRRKGTWAVVLS"
BASE COUNT          806 a         2178 c         2215 g          754 t
ORIGIN      
        1 ttcgggtttc gactgggcgg ccctggggca tcaccaccgg ctggaggtcg tgacgcgcga
       61 ggacgggagc ccggcggggg cctacagcgg ctcgccgacg ggccggggcc tggacgagac
      121 cgggccgcgg cacttcctga aggtcacgct cgaggagggc tcgccggccc gcgtcgagac
      181 gctctccgcc gactgccgga aggtgctcga cctcgagctc gacgcggccg gccgggacgg
      241 cgagggcctg cgggacgccg cccttgcgct cctcctcgag gcgggcgcga cgccgggcga
      301 cctcgtgcgg ctcaccgtcg tcggccggcc cgcggccgcg acccgggctt cccaggccct
      361 gggcgacctg aaggggctcg tcgctcacct cgcgctccgc gaccggaccc tcggcgacgc
      421 ggaggcccgg caggaccgtg ggacggcgga gggacggttc gccctcgagc tggagtcgcg
      481 cctcgccgcg acggaggacc cccgggtgcg ccgggtcctc gagctcgcgt ccgtcctggg
      541 gcgcgaggcg ctcctcggcc gcgtgccggc tccccccgcc gaggaggacc tctgaggatc
      601 gagcgcgtcg tcgtcgaggg cttcgggccg ctcgaagggc tcgacctcgc gtggggcgac
      661 ggcgagctcc tgctcgtcct cgagccgaac gagaccggga agtcgacgct ctgcgaagcg
      721 atcacggccg cgctctacgg gctgccgaag ggacgctcgg cgggagggcg ttcgcgcgag
      781 tcccgacggc cgaggagcgg cgcgccgacc cggctccgcc tcgacgtgtc tgcggcggcc
      841 ggacgcctgt cgatcgagag ggacttcgac gaggggacgc tgcgcgtcgt ggaccgcgac
      901 cgcggggaag accggaccgc ggagttcctc cgcgcgggcg gccgggatgc cgtcggagag
      961 aagctgaccg gtttgacgga ggcgctcttc cggacgacgg cctacgtcgg gcagaacgtc
     1021 ctcgacggcg acgccctcga ctcctcgctc accgtcgagc tcgcccgaat cgccgactcg
     1081 ggcggaggcg aggcgagcgt cgtccgggcg ctccggcttc tcgaggcggc ccgggcccgg
     1141 atgcccgacg ccaccacggg ggcgcaggtc tcggtcgaga ccgagatcgt gcgggcgagc
     1201 cgtcgggtgg aagagaagcg ggccgaggtg gcccggctcg cgaaggcgcg ggaggcggcc
     1261 gcggaggccg ccgtgaggct cgcttcgcgg accgcggaga gggacgccgt ccgaagggag
     1321 gcggcgctcg cggaggtggc cgtcgtcgag agcgagcgcc gcagcctctc ggccgagctg
     1381 gagcggagga cggcggagca gcggcgtcgg ggcgagctgg ccgccgaggc gaggtcgctc
     1441 gagagggagg cggccctttt cgcgcccgaa gcgctcgccg cggtcgacgc ccttcgctcc
     1501 gctcgcggct cccggcccga ggcgctcgtc gcggcccgcg cggcgatcgc cgcggagcgc
     1561 gccgccacgg aagcggagga ccgcgaaagg cggaagaggg cgggcggcct cgccgacctc
     1621 gatccggagg cgcgggccct cctccgcagg cacctcgagg cgtcccggga ggcggaatcc
     1681 gagtcggagg cggccgagaa ggccctcgag gccgagtggc aggagctccg gagggacggc
     1741 ctcgccgagg acctccgccg gctcgacggt ctcggcgccg ccgacaggga cttcgtcgtc
     1801 gcggcggagg agagccggca ggggctcgag ctcgaagggg tgcagctcga ccgccggttc
     1861 gcagacgccg gagcgcgcgc ctcgatcgtc gcgggcgaac gccgggtcct cgtccggagg
     1921 gcccgggcgc tcgtcgtcgc ggccagcctc gtggcgcccc tcgccgtcgg gacgttggtc
     1981 cgacgcggcc ggctggacga ccctctcgtg ctgatgctct tcgcgttcgg tgtcgtcctc
     2041 gcgctctacg gcggaatcgc ctgggcacgc ggagaccgct acggccggga ggaggaagcc
     2101 cgcttccggc aggaggaggc cgccgcccgc gccgaggccc aggagacgcg gcggcgcctg
     2161 tccgagctgc gcctgcggct cgagcagatc gcccgcggcg cgggattcaa ggactccgcg
     2221 ggcctcctga aggcgcaacg gaaggcgcgc gccgcggacg aggagaggca gcggctcgtc
     2281 gagaagcggg tgcgcctctt cgcggccggg gagcgcctcg ccgccgccgc gaagggactc
     2341 gaggcctttc gaggcccgct cgggctcgcg tcgggcctgc cgacggccga cgagtcgagg
     2401 cgggtcctcc agatgctcac cgacctcgag aaggcggact cggccctcgc ggggcgccgc
     2461 gaggtcgcgg agcgcgaggg ggagcgcctc gaggcggagg agcgcgagct ccgccggctc
     2521 gaggaagagc tggccgtcct cctcgcgcgg ctcgaggtcc cttcgggcct ctccctgccg
     2581 gagtccctcc tcttcgtcga ggcggggcgc gcccgcgccg cccggcggcg cgagatcctc
     2641 gaggtcgagc ttcccgcgct ggccgtggcg ccgctgccgg acggggacga ggccttcgag
     2701 gagcgcgtcc ggctcctcgc cgcggaggtc gagatgcgcc tcgacgtcct cggggcggtc
     2761 ctttcggacc tctccgtgcc ggccgagccg gaggcggccc ggcgcgcggc cgaggctctc
     2821 cgcgagcggg ccgcgcgcct ggaggacgag cggcaggcgg ccgagcggga gctcgcgcag
     2881 agggcgatgg aggggggcga acgagggcgg gaagcggagg agcagctggg ggccctcgac
     2941 gtcgcccggg agcgggcggt cctctaccgg gacggcctcg acgccgcgcg cgaggtgctc
     3001 gcttcggccg cgacgacgac gtacggggac ttccgcgcgg gcctcgcgcg cgcgtcccgg
     3061 gagatcctgg cgcgctgccg cctgccctac gaggcgctcg agttcgggga ggacctgtcg
     3121 gtctcggtcg tcggccgcgg cggccgcccg gtgacgcgcg ccgagctcgg cgcttcggtc
     3181 tcgaccggcg cccgcgagca gctccacctc gttgcccgtc tcgcggccct gcgccacctc
     3241 ggcaccggcc cccgcggcct cccgctcctt ctcgacgacc cgctcgtcgg cgcggacgac
     3301 gaccgcttcg gtgccgtcct agaattcctc ctcgacgacg tcctcgccga gcgtccggtc
     3361 ctcctcgtct cctgccaccg gtcccggcac gaacgctggc tcggggcgct ctcgcccgag
     3421 cgggcggcgc gcgtccggcg ggtgaggctc tcgggcgggg ggcggccgga gccggagcgc
     3481 gaattgacgc cggcgccggg cggcgaatag actccctgcc ttggcccggg cgagccgggc
     3541 cgattccagt cgggggccca ggtcttcagg aggcgtcggc ttcctcgtcc gggccgaacc
     3601 gccttcggag gttgcgtttc gtgaaaaggc ccacaggctg gcgtctcgcc ctgacgctgg
     3661 tcgtcctgat cggatccatc gcggcctacg tctacagggg ccgcgaggcc ctcaagaaga
     3721 accctacggc acctccgaac ccgtcgatcg gcgacgtcct gaagaacggc ctgaagctcg
     3781 ggctggacct tcgcggcggc atccacctcg tcatgcaggt gaaggcggag gacgccctcc
     3841 gcgcggaggc gaacgacgcc gccgagcacc tgaagaccga ggccggcaag cgcggcgtca
     3901 ccctcgggac ggtcaacccg gcgacccacg aggggttcac gatcacgatg ggcgaggacg
     3961 tcagcccggc ggccgtctcc gacctcgtca agagcgccct ctcgccgacc gactgggcct
     4021 cggaccagag cggccgcgag atgcgcgtga cgttccgcga cccggcccgg atcgagatcg
     4081 agggccagac gctccggcag gccgtcgaga cgatccggaa ccgcgtcgac gccctcggcg
     4141 tcgccgagcc ggcgatccag ccccagggga acgaccggat cgtcatccag ctgcccggcg
     4201 tcgacgaccc ggcccgggtg aaggacatca tcgggacgac cggcctcctc gagctgaaga
     4261 tcgtcgacct gaagggcggc ccctactcct cgcccgaggc ggccgcggcc gcctacggcg
     4321 ggacgatccc ggcgaacctc gagctcgtcg aggggaacgc cgaggacgag caggcccgga
     4381 cgcgaacgac cgtctactac gtcctcgagc gggcctccgc cgtcacggga cgtgacctga
     4441 agaacgcccg cgtcgggcag gacaagttca accagccggc cgtcgagttc ttcctgaacg
     4501 cggcgggggc cgagaagttc ggccgggtga ccggcgacaa cgtcggccgg cagctcgcga
     4561 tcgtcctcga caagagggtc cagtcggcgc cgaacatcaa gagccagatc aacgacaacg
     4621 ggatcatcga ggggaacttc acgcccgagc gggccgactc cctcgcgctc gtcctccgtt
     4681 ccggcgccct cccggcgcag ctcgtcatcc tcgaggagcg gacggtcggg ccgtcgctcg
     4741 gcctcgactc gatcaagcag gggatcctcg ccgcgctcgt cgggatcgcc ctcgtcttca
     4801 tcgccgtcgt cgtctactac aacctcgccg gtctcaacgc ggtcctcgcc ctgacgatga
     4861 acgcgatcat cctcctcggc gcgatggcct ggatcaacgc cacgctgacg ctccccggca
     4921 tcgcgggctt catcctgacg atcggcatgg cggtcgacgc gaacgtcctc atcttcgagc
     4981 ggatccgcga ggagctcgac gccggcaagg gcgccaaggc cgcgatcgac ctcggcttca
     5041 agaaggcgct cagcgccatc gtcgactcca acctgacgac catcgcggcc gcggcgttcc
     5101 tcttccagtt cggcaccggc cccgtcaagg gcttcgccgt gactctcgtc atcggcctct
     5161 gcgcctccat gttcaccgcc gtcttcgtct cgcacctcct cttcgacctc ctctacgggt
     5221 cgaaggagaa ggtcgagacc atctcgatct gagggacacc gtgaggctgc tccgcaatcc
     5281 caacttcgac ttcctcaagc tccagaagcc cttcgtcatc ggctcgatcg tcgccgcggt
     5341 ggtctcggcc ggcgccatcg ccacgaaggg gctcaactgg ggcgtcgact tcaccggcgg
     5401 cgcccagatc gtctacgcgt tccagcagac ccccgacgag gaccaggtcc gcaagatcgt
     5461 ggaaggggcg ggcgtccccg tcacgtcggt ccagcgctac gacaaggccg agaagaacca
     5521 gatcctcctc cgcgtcccga tggagcagaa ggaggggagg gacatctccg ccgaagtgac
     5581 gccggcgctc gtcaaggcgc tctcccccgc ggggcgggac gagggagcct tcgacctgaa
     5641 cctgaacggc gccgacccgc tctccgccaa gctccagcag gatgacccgg agaagatggc
     5701 cggccgggcc gacgcgaacc cgaccgtcga gtacggccgg atcgcccagt cgatcatcgc
     5761 gcagcgctcg caggccgggc tcttcgcgag cctcgacgcc gccgcggcga cgcccggcct
     5821 ttcgcccgcc gtcgcctcct ggctgaagga gaagaccgtc gccggccccg tgacgctcat
     5881 cagcgccgag tcggtcggcc ccgcggtcgg caaggacctc cgccggaagg ggacctgggc
     5941 cgtcgtcctc tcg
//
LOCUS       DAOTEZ010000266         5937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_9590_length_5937_cov_4.806188, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000266 DAOTEZ010000000
VERSION     DAOTEZ010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5937
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9590_length_5937_cov_4.806188"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="P5164_13460"
     CDS             <1..412
                     /locus_tag="P5164_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44945.1"
                     /translation="GTLLRDFDPEDLSTLGDFVQALGSGLPSTLCAAAQSVGGRPDPV
                     CTLVRHMVAGATDVSYGDGMKARRGFYRKLSDLPSPLVAVRFLWTATLLTRSQIDTIY
                     GPPRSRLGYLGRQLARPFDLVARYLRARRIRKSG"
     gene            1433..1681
                     /locus_tag="P5164_13465"
     CDS             1433..1681
                     /locus_tag="P5164_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44946.1"
                     /translation="MTISLPDSLARRFQAAVPARRRSSTVARLVEAELVRREDELAIA
                     CAAANADSVLAEEIVEWQSFDDAVSPPRPAARSRKAKK"
     gene            1678..2007
                     /locus_tag="P5164_13470"
     CDS             1678..2007
                     /locus_tag="P5164_13470"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004164066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRY44947.1"
                     /translation="MTPARGEIWWVRLDPTLGSEIRKTRPAVVVTADALNRMRQTVVV
                     VPLSTAAAAHPPVTVPVHCRGKRVVAVVDQVRAISRERLVSKIEDAREEELLAVGEAL
                     ARILELP"
     gene            2753..5734
                     /locus_tag="P5164_13475"
     CDS             2753..5734
                     /locus_tag="P5164_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44948.1"
                     /translation="MESGPAGTQLPFYLLWYADAACTSFLPKPGGGKLYYEEVRFSPS
                     PEWALARLAGTVPAGAAAVSFQFSILLDRINGPATALFDDASFFEGDPTPVVTSFTAN
                     PAEAFAGQNVTLSWVTESTGVCIDGIGNGCAVPTALPASGSTTVAPTATTTYTLRASR
                     GGRTTMATRTVTVRPRDTAPARYEFTATTGALVFSFPSAAITRAFAWDAAGLLVGDPA
                     DPRARRVAQAIVGEKLGPEALSAYLEAALSTALDAAEPLAVVPLARGAAVGGGGGVKG
                     IEAIGQTKVGAVVVLDFVGTSKNPQSLPGIVDLSFGLADQALHLPLGLLDATDLSRMT
                     AERSYLIVPPAAVDLYELRGFELCVAPHAVTIVARRIGGGPSGEVDASRTAALALEPA
                     ATGCPTSLNLPAGEPGRVAGTPRGAATTVYVTRPDVPVAGAGVGGLAAAEPCPVRLQA
                     QESASGATFADVEVPEARESRVDLGVVPAAEWHLLVTSRCAGRVGLLQAETPARSDAN
                     TRVLPIALDVSTATARYTTELAITNRSSQRLGLGLTFQPSLGSRAGGGSVDLAIGPGQ
                     QLRFDDAIAALRSAGLAIPQEPTDPKGGSLRAEFRAAGALDSADVAVTARTTSPVPGG
                     AAGLAYSGEERERASGATKAVFGLRQTERDRSNLALYNPGDVPVTLRVTIHSGSGDGR
                     TAVVADGVALEPLGWKQIDKVLVSAGFEQGWAVVERTSATGVFGAYGVVNDNGTNDGS
                     FIAGTDRRATGSRVAIPVIVETTAFRSELVVANSATEPATLRMSYVESLRPDAAQGGE
                     AEIVVPPATQLLIGDAAAFLRERGVAIPTAAQGGAAGTIRVKVAGVPLGSVFVAARVS
                     SQSEAGSYGLFVPGIPEGEAARVDAVVAGLVSDERSRSNVAIANVGDAADGSVTLEIR
                     CFDGEAGGVERGGVEVATLAPGQWKQLSGILRTKGIRSGWATVRRSAGKAIWLAYGIV
                     NDGAAPGQGTGDGAVVPMTPR"
BASE COUNT          849 a         1957 c         2214 g          917 t
ORIGIN      
        1 cggcaccctc ctacgcgact tcgaccccga ggacctgtca acgctggggg acttcgttca
       61 ggccttgggg tcaggtctac catctacact ctgcgcagcc gcgcagagtg tcggtggcag
      121 acctgacccc gtttgcacgc tcgtgcggca catggtggcc ggggcgacgg acgtctccta
      181 tggcgacggg atgaaggcgc gtcgcggctt ctatcggaag ttgtcggacc tcccatctcc
      241 cctcgtcgcc gtgcgcttcc tctggaccgc aacccttctg acgcggagcc agatagacac
      301 gatctatgga ccgccccgct cccgcctcgg ctacctcggc cgccagctcg ctcgcccctt
      361 cgacctcgtc gcccgctacc tgagagcgag gagaattcgg aagagcggtt agggcagcaa
      421 gggggggcag gccagagctg ggacagaatg gggtcaggtc taccatctac actctgcgcg
      481 gccgcgttag cccgacttcc gcagttcgag acgatttcgg gcctgaggcg aggcgtttct
      541 cggcgtaagt gactgaggct tcagggggcg attcgagaac cgcgatgtag tgccgaccta
      601 cccccttgcc gtgcggggtg cggcctgcgt tcaatgacgc aggaaccggc acgatgtacc
      661 tgcgacatgc gacgcgacgc aaggacggca agacgcacag gacagaatgg ggtcaggtct
      721 accatctaca ctctgcgcgg ccgcgttagc ttcaggtgct ggcccggtcc ccgttcttcc
      781 cggtcttgcc tcgtgagtct gaggggaggg aggctgtgac ggggcggcct ccggcctctt
      841 ctcagcgagc ggtctctcga ctcgttcgga gtcgtgaacg aggagcggct cgacctgcgt
      901 gtccaggaag cggtccggcg aatcgtcgac gagtttgagc cgtcgatggt cctgctgttc
      961 ggctcgcgcg cccgggccga ggcgaggccg gacagcgacg tcgaccttat cgtcgtctgg
     1021 aaggacgaga acccgcctcc gaagcgtgcc gcggcggtcc ggcgtgcgct tggccgcgtt
     1081 ggggtcccgc tggatctcgc agtcgtcacg ccgtccgaat ttgcgcggtt gaaggaaggg
     1141 cagggaggcc tcattcacgc ggccgctcgg gagggtgtcg tacttcatgc cgcctttcgc
     1201 gccgagggcc gtcatcgagg agctcgtgac gatcgcccgc cgcgacctgc gagccgcgga
     1261 gcttctggcg agcgagcctt gataccgggg cctgacccct ggggttccgg gggcctcgct
     1321 tcgtgactac cgcggccgcg tagagtgtag atagtagatc tgaccccagg gcccgggagc
     1381 cttcaggtcg tgcgggcttc gccgacagca ttcataattc ataaggatga gagtgaccat
     1441 ctccctcccg gattccctgg ctcgccgctt tcaggcggct gttccggccc gccggaggtc
     1501 ctccaccgtg gcacgcctcg tcgaggcaga gctggtgagg cgcgaggacg agctcgccat
     1561 tgcatgcgcc gctgcgaacg cggattccgt cctcgcagag gagatcgtgg agtggcagtc
     1621 gttcgacgat gccgtctcgc cgcccaggcc cgccgcgcga agccgtaagg cgaagaagtg
     1681 acgccggcgc gcggggagat ctggtgggtc cgcctggacc caaccctcgg ctccgagatc
     1741 cggaagacga gaccggccgt cgtcgtgacc gccgatgcgc tgaaccggat gcggcagacg
     1801 gtcgtcgtcg taccgctgtc gacggctgct gcggcgcacc ctccggtgac ggtgcccgtc
     1861 cactgccgcg ggaagcgggt ggtcgccgtc gtcgaccagg tccgggcaat cagtcgagag
     1921 cgcctggtct cgaagatcga ggatgcacgg gaggaggagc ttctcgccgt cggggaggca
     1981 ctcgcgcgga tactcgagct gccctgacac ctaccggagc gaagtccgcc gaggccatga
     2041 tgatgaaagg gcgatttccc accacgagct cccggcggcg ctcctgatgg cgttggagca
     2101 agcccttcgc gggccgcttg ccgggtatcg gccgctgcgc gtcttcctgt tcgggtcccg
     2161 cgcgacggga tcggacagaa tgaggtcagg tctaccatct acactctgcg cggcggcgcg
     2221 gagtgtagat ggtagacctg accccgtagc accgtagcgc ctctcgcggc gcgtctttcg
     2281 atgaggcacg agcgaggccg ccggcgataa tcgttcggtt cggatgaatc tcgcggaggt
     2341 cgcgccgtcc gacgccaggt tccatgcttc gtcgcgaatc gatcgacgcc gaccgccgtg
     2401 aatccggcga ggaggagagg ccatgaagag catcctgacc cggaaccgca cgaagacggt
     2461 ggtcggcgcc acctccttgc tcctgctctc tacggcggcc ggctccctgt tcgcgcagga
     2521 gcggatccgg aatggcgggt tctcggacgg gttgaacggt tgggtggggt tgagcgaggg
     2581 aacgagcgga ggcacgacgc ggacgtatgg gcaggtgccg gacggtggcg gggcggctcg
     2641 ggtgacggtc gcggtggagt cgagccaggt ggggacaggc ggccaagcgt ggctgacgca
     2701 gtgcgctccc gtcaatccgg gaagcgcgta tgttgcttct ggtcgagcac ggatggagtc
     2761 gggtccggcc gggacgcagc ttcccttcta cctcctctgg tacgccgacg cggcgtgcac
     2821 gagcttcctt ccgaaaccgg gaggcggcaa gctctactac gaagaggtcc gcttctcgcc
     2881 gagcccagag tgggctctgg ctcggcttgc cggaacggtt ccggcgggtg ccgcggccgt
     2941 ctcgttccag ttctcgatcc tcctcgacag gatcaacgga cccgccacgg ccctcttcga
     3001 cgatgcctcg ttcttcgagg gagacccgac accagtcgtg acctcgttca cggcgaatcc
     3061 ggccgaggcg ttcgctgggc agaacgtgac gctgagctgg gtgacggagt cgacgggggt
     3121 ctgcatcgat ggaatcggaa atggatgcgc cgttccgacg gcgctgccgg cctcgggctc
     3181 cacgacggtt gcgccgacgg cgacgaccac ttacacgctc cgggcgtcac gcgggggccg
     3241 gacgacgatg gcgacgcgga cggtgaccgt tcggccgcgg gacacggcgc cggcccgcta
     3301 cgagttcacg gcgacgaccg gggcgctggt cttctcgttc ccgagcgcgg cgatcacgcg
     3361 cgccttcgcg tgggacgcgg ccgggctcct cgtgggcgat ccggccgacc cgcgtgcccg
     3421 gcgggtggcg caggcgatcg tcggagagaa gctcgggccg gaggccctct ccgcgtacct
     3481 ggaggccgcg ctctcgacgg cgctcgacgc ggccgagccg ctcgcggtgg tgccgctcgc
     3541 gcgcggggcg gcggtggggg gcggaggcgg cgtgaaaggc atcgaggcga tcggccagac
     3601 gaaggtcggg gccgtcgtcg tcctcgactt cgtcggaacg tcgaagaacc cgcagagcct
     3661 gccgggcatc gtcgacctgt ccttcggcct ggcagaccag gcgctgcacc tcccgctcgg
     3721 gctgctcgac gcgaccgacc tctcgcggat gacggccgag aggagctacc tgatcgttcc
     3781 cccggcggcc gtcgacctct acgagctccg cggcttcgag ctctgcgtgg cgcctcacgc
     3841 ggtgacgatc gtggcacgga ggatcggcgg gggaccgtcg ggtgaggtcg atgccagccg
     3901 gaccgcggcg ctggcgctcg agcccgcggc gaccggctgc ccgacgagcc tgaacctccc
     3961 ggcgggcgag ccggggcggg tggccggcac gccgcggggc gcggcgacga cggtctacgt
     4021 gacgcggccc gacgtgccgg tcgcgggggc gggcgtcggg gggctcgcgg cggccgaacc
     4081 ctgcccggtg cggctgcagg cgcaggagag cgcctcgggc gcgacgttcg cggacgtcga
     4141 ggtcccggag gcgcgggaga gccgcgtcga cctgggcgtc gttccggcgg cggagtggca
     4201 cctcctcgtg acgtcgcgct gcgcgggccg cgtcgggctc ctccaggcgg agactccggc
     4261 gaggagcgac gcgaacacgc gcgtcctgcc gatcgcactc gacgtctcga cggcgacggc
     4321 gcgctacacg acggagctcg cgatcacgaa ccgctcctcg cagcgcctcg ggctcggcct
     4381 gacgttccag ccgtcgctcg ggtcgcgtgc cgggggcggc tctgtcgatc tcgcgatcgg
     4441 cccggggcag cagctccggt tcgacgacgc gatcgcggcg ctccggtcgg cggggctcgc
     4501 gatcccgcaa gagccaaccg atccgaaggg cggatcgctt cgggccgagt tccgggcggc
     4561 gggcgccctc gactccgcgg acgtcgcggt gacggcacgc acgacctccc ccgtgccggg
     4621 cggggcggcc ggcctcgcgt actcggggga ggagcgggag cgcgcgtcgg gcgcgacgaa
     4681 ggccgtcttc ggcctccggc agacggaacg cgaccgctcg aacctcgcgc tctacaaccc
     4741 cggcgacgtc ccggtgacgc tgcgcgtgac gatccactcg gggagcgggg acggtcggac
     4801 ggccgtagtt gcggacgggg tggcgctgga gccgctcggc tggaagcaga tcgacaaggt
     4861 cctcgtctcc gcgggtttcg agcagggctg ggcggtcgta gagcggacgt cggcgacggg
     4921 cgttttcggc gcctacggcg tcgtgaacga caacgggacc aacgacggct cgttcatcgc
     4981 cgggaccgac cgccgggcga ccggcagccg cgtcgcgata ccggtgatcg tcgagacgac
     5041 ggcgttccgg tcggagctgg tcgtggcgaa ctccgcgacc gagccggcga cgctccggat
     5101 gtcgtacgtc gagtcgctcc gaccggacgc ggcgcagggg ggcgaggcgg agatcgtcgt
     5161 tccgccggcg acgcagcttc tcatcggcga cgcggccgcg ttcctgaggg agcgcggggt
     5221 cgcgatcccg acggcggcgc agggcggcgc cgcagggacg atccgcgtga aggtcgcagg
     5281 cgtgccgctc gggagcgtgt tcgtggccgc gcgagtctcc tcgcagtcgg aggccggctc
     5341 ctacgggctc ttcgtcccgg gaattcccga gggcgaggcg gcgcgcgtgg acgcggtcgt
     5401 cgcgggtctc gtgtcggacg agcgaagccg ctcgaacgtg gcgattgcaa acgtcggcga
     5461 cgccgctgac ggctcggtga cgctcgagat ccgctgcttc gacggggagg cggggggagt
     5521 cgagcggggc ggagtcgagg tggcgacgct cgcgccgggg cagtggaagc agctctccgg
     5581 catcctccgg acgaagggga tccgctcggg ctgggcgacc gtccggcggt cggccggcaa
     5641 ggcgatctgg ctggcgtacg ggatcgtgaa cgacggagcc gccccgggcc agggaaccgg
     5701 cgacggggcc gtcgtcccga tgaccccccg ttagagccag gggtcaagag ccaggggtca
     5761 ggtctaccat ctacactcta cgccgccgcg tagagtgtag atggtagacc tgaccccacc
     5821 ctgccctccc tttctccgcg gggggataat ccgcgccctt ccgtccggcg gaaccggggg
     5881 tagccatgga ttcgatcgtc atccgcggtg cgcggcagca caacctgaag aacgtct
//
LOCUS       DAOTEZ010000267         5922 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_9619_length_5922_cov_5.661837, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000267 DAOTEZ010000000
VERSION     DAOTEZ010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5922
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9619_length_5922_cov_5.661837"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(341..1930)
                     /locus_tag="P5164_13480"
     CDS             complement(341..1930)
                     /locus_tag="P5164_13480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRY44949.1"
                     /translation="MTEDLLRARSSLSSLATRLVSRLADSRAAYSDDDLDRDLGLVLS
                     EILAVSGARVGWLGVFCDGEGRVPSPEEHAARATHVRGRTAGLTLGWSETTRGTLGQR
                     SQLPGLRTHVARTRRNAQVFWDAEAVRELEEKGVLRAATREDEIELLERMRRETAPAG
                     EPPGAWERRIDALVERARDRPADERYFEQVRRMWDERSPIVRRDGMECTLEEWFPTAV
                     FPVVRRFGEEEQCVGEVVLALVAGRRPEEPLAQERMERVQEVVDLVSVPLAVAVSARQ
                     LAEGLARERALRDELSRERRLSALRGLTGQLAHDVNNAAAVVDSSLRAAQRALGEKDV
                     VRLAREEPPLERALERAWEELTTARRALESVTDTVQRSRTSVAADLFRPREVDLNAEL
                     ERWALPGVTRRRGEGVGTVVCDPELLYRAVLNAVANARRAVEERVRSAGGAGEVVVST
                     ERTGASVVVAIADDGVGMPPEALAKLEAGEAFTTKVGGAGVTGLGTGILRTIAETHGG
                     SVTWTSAPGRGTTVRIHLPAA"
     gene            complement(1959..3002)
                     /locus_tag="P5164_13485"
     CDS             complement(1959..3002)
                     /locus_tag="P5164_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44950.1"
                     /translation="MSQAVPDGAGPAVEVGPFREGDGEGIAALFREVYGDGYPVKVFY
                     DPVALARANAEGETYSIVGRGAAGEVLGVSHLYRSAPYRRLFELGAGLVRKESRNLGL
                     VARLIGYALNEWLPGREEIEEVFGEAVCNHTHSQRVGIALGFVETALEVALMPAEAYT
                     KERSARGRVAALLSFRCYRPRPHTVFLPSPYRDELRFLYAALDDRRDLAEAHAPLPED
                     AATEATMEVFDFARVARIAVPRAGADLAARLDALEAEAGRKGVEVFQVWLNAAEPQVG
                     AAAALLRERGYFLGGVLPRWLDTDGLLMQRLLCPPDFGENQLHSDRARDILALVRRDQ
                     ERASARPAAGSPA"
     gene            3157..>5922
                     /locus_tag="P5164_13490"
     CDS             3157..>5922
                     /locus_tag="P5164_13490"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY44951.1"
                     /translation="MLFALRRLSLGLVLAALASAVLLLSDAHQRRPKEKPRPRVAVFQ
                     IASRPVLDDSVRGVLEGLAAAGFLEGTTLEVKRYNAEGDLPTANAIAREIVGGGFDVA
                     ITVSTPALHALANANRDGRVLHVFCTVTDPFEAKVGLAPGDPLGHPRHLVGLGTFQPV
                     RELLRLARRLRPELKTVGTVWCPSESSAEACVKLAREESAALGLRLLEANADAPADVA
                     EAARSLTSRGVEALWLGGDNVVETAVSSLVKVAREAKVPLLANAPTHAEAGALAGLGA
                     DYVEVGRRAGDLASRLLAGLDPATVRVENVAPPLLALNLSALSGLRDRWSVPEDLRSE
                     AAVVIDGTGVKTERSAAPEAAVTPTAAGAGRRRITLVQLVSGPAIDATREGMLRGLSD
                     GGFVPGRDVDVKVRDAQGELSALPALVDAARADRADLVLTITTPALQAAMARLSDRPV
                     VFGLSIDPLLAGDGGTHERHRPNVAGIYDRSPFEPLLDLLRQLLPRARKIGTLFNPAE
                     PNSVLFRDELARVARSRGLALVAVASDSPAGVADAALALVGRGVDAVFQVNDNLHEAS
                     FSGIAAAARRARVPVFAFSSPLVHEGAAIALANDHSDGGREAGRIAARVLRGESPASI
                     PYRGITRTVLAVSPEAAAAAGLALPADLLSRAAVVAGRPAAAATPSVASRAAPPPLGR
                     TANLQVLHFAESTMVEEAMEGLRAELPRTGLVEGRDYELTVRNAQGEMSNLVSLLDAA
                     LATRPEVLLVTSTPTLQAALKRVKGVPIVFSIVANPVLAGAGTSFAEHAPNVTGISTE
                     SDFAGMARVVRECLPRARRVGTLFNPGETNSVFNRDGTARALAAVGIELVSVATSTPA
                     EVPDAALALAGRGLDAICQVNGNLHDSGFSGIAQAARKAKLPLFAFTSAQALSGGAAV
                     AVARDYVQ"
BASE COUNT          715 a         2335 c         2119 g          753 t
ORIGIN      
        1 accggcttcg cggacgagga ccgcaccgtc atcgagaagg cgggcttcag ccgcgtcctc
       61 ggcaagccgt gccctcccgc gctcctgtgg gagacgctga aggagggggc gaaggcgcta
      121 aggaaccggg acgtgaacct cggcgactga agtttcccgg ggggaccgcg ccctgacggg
      181 cggcggccgg tccccccgga ccccctccgc gccggctcat gcacgatcgg ggaccgcgcc
      241 ctgacgggcg gcggccggtc cccccggacc ccctccgcgc cggctcatgc acgatcgggg
      301 accgcgccct gacgggcggc ggccggtccc cccgggcggc ctacgcggcc gggaggtgga
      361 tccggacggt cgtgcctcgc cccggggcgc tcgtccaggt caccgagccg ccgtgcgtct
      421 ccgcgatcgt ccggaggatg ccggtcccca ggccggtcac gccggccccg ccgaccttcg
      481 tcgtgaacgc ctcgcccgcc tcgagcttcg cgagcgcctc cggcggcatc ccgacgccgt
      541 cgtccgcgat cgccacgact acgctcgcgc cggtccgctc cgtcgagacg acgacctccc
      601 ccgcgccgcc cgctgaccgg actcgctcct ccacggcgcg ccgcgcgttc gcgacggcgt
      661 tgaggacggc ccggtagagg agctccgggt cgcagacgac cgtgccgacg ccctcccccc
      721 tccggcgcgt cacgcccggg agcgcccagc gctcgagctc ggcgttcagg tcgacctcgc
      781 gcggccggaa gaggtcggcc gcgacggagg tccggcttcg ctggacggtg tcggtgacgc
      841 tctcgagcgc gcgccgcgcg gtcgtcagct cctcccaggc ccgctcgagg gccctctcga
      901 gcggcggctc ctcgcgcgcg agcctcacga cgtccttctc cccgagcgcg cgctgcgccg
      961 cacggagcga ggagtcgacg acggcggcgg cgttgttcac gtcgtgggcg agctggccgg
     1021 tcaggccgcg gagcgccgag agccggcgct cgcgggagag ctcgtcgcgg agggcgcgct
     1081 cgcgcgccag cccctcggcg agctggcgcg cggagacggc gacggcgagg gggaccgaga
     1141 cgaggtcgac gacctcctgg acgcgctcca tccgctcctg ggcgagcggc tcctcggggc
     1201 gtcgccccgc gacgagcgcg aggacgacct cgccgacgca ctgctcttcc tcgccgaacc
     1261 gccgcacgac ggggaagacc gccgtcggga accactcctc gagcgtgcac tccatcccgt
     1321 cgcggcggac gatcgggctc cgctcgtccc acatcctccg gacctgctcg aaataccgct
     1381 cgtccgccgg ccggtcgcgg gccctctcga cgagcgcgtc gatccgacgt tcccacgctc
     1441 cgggcggctc gccggcgggc gccgtctccc gcctcatccg ttcgaggagc tcgatctcgt
     1501 cctcccgcgt cgcggcccgg aggacgccct tctcctcgag ctcccggacg gcctccgcgt
     1561 cccagaagac ctgggcgttc cggcgcgtcc gggccacgtg cgtccggagg cccggcagct
     1621 ggctgcgctg tccgagcgtc ccgcgcgtcg tctcggacca tccgagcgtc aggcccgccg
     1681 tccggcccct cacgtgcgtc gccctcgcgg cgtgctcctc cggggagggg acgcgcccct
     1741 ccccgtcgca gaagacgccg agccagccga cgcgcgcccc cgagacggcg aggatctcgg
     1801 agaggacgag cccgaggtcg cggtcgaggt cgtcgtcgga gtaggccgcc cgcgagtcgg
     1861 cgaggcggga gacgaggcgc gtcgcgagcg aggagaggga cgaccgggcg cggaggaggt
     1921 cctccgtcat cccgggcctc cccggaccgg tctcgtcgtc acgcgggcga cccggccgcc
     1981 gggcgggcgg aggcgcgctc ctggtcccgc cggacgagcg cgaggatgtc gcgcgccctg
     2041 tcggagtgga gctggttctc gccgaagtcg ggcgggcaga ggagccgctg catcagcagg
     2101 ccgtccgtgt cgagccaccg cggcagcacg ccgccgagga agtagccccg ctcccgcagc
     2161 agcgccgccg cggcgccgac ctgcggctcc gcggcgttca gccagacctg gaagacctcg
     2221 acccccttcc gcccggcctc cgcctcgagc gcgtcgagcc gcgcggcgag gtcggctccg
     2281 gcgcgcggga cggcgatccg cgcgacgcgg gcgaagtcga agacctccat cgtcgcctcg
     2341 gtcgccgcgt cctcggggag cggcgcgtgc gcctcggcga ggtcgcgccg gtcgtcgagc
     2401 gccgcgtaga gaaagcgcag ctcgtcccga tagggcgaag ggaggaagac cgtgtgcggc
     2461 ctcggccggt agcagcggaa cgacaggagc gccgcgacgc gcccgcgggc gctccgctcc
     2521 ttcgtgtagg cctcggcggg catcagcgcg acctcgaggg ccgtctcgac gaatccgagg
     2581 gcgatcccga ccctctgcga atgggtgtgg ttgcagaccg cctcgccgaa gacctcctcg
     2641 atctcctccc gccccggaag ccactcgttc agcgcgtagc cgatcagcct cgcgacgagg
     2701 ccgaggttgc ggctctcctt ccggacgaga ccggcgccca gctcgaagag cctccggtac
     2761 ggcgcggagc gatagaggtg cgacaccccg aggacctccc ccgccgcgcc gcggccgacg
     2821 atcgagtacg tctcgccctc cgcgttggcc cgagcgagag ccacggggtc gtagaagacc
     2881 ttcaccgggt agccgtcccc gtagacctcg cggaagagcg cggcgatccc ctcgccgtcc
     2941 ccctcgcgga acggtccgac ctccacggcc ggtccggctc cgtcgggcac cgcctgcgac
     3001 atgggctcct ccctcgacgg gattctgggg agcgcagggg acgcggtcaa tcggcggcac
     3061 ctcccgcccc gcgcaggccc cgccccgcgc gcgccgctag aatgcccgat ggtcggctcg
     3121 cttcccgtgg gggccgcggg ccggggggag gacacgttgc tcttcgctct caggcggctc
     3181 tcgctcgggc tcgtcctcgc ggcgctcgcg tcggccgtcc tcctcctctc cgacgcgcac
     3241 cagcggcggc cgaaggagaa gccgcgtccg cgcgtggcgg tcttccagat cgcgagccgg
     3301 ccggtcctgg acgactcggt ccgcggcgtc ctcgaagggc tcgcggccgc cggcttcctc
     3361 gaggggacga cgctcgaggt gaagcgctac aacgccgagg gggacctgcc gacggccaac
     3421 gcgatcgcgc gggagatcgt gggcggcggc ttcgacgtcg cgatcacggt gtcgacgccc
     3481 gccctccacg cgctggcgaa cgcgaaccgc gacgggaggg tcctccacgt cttctgcacc
     3541 gtcaccgacc cgttcgaggc gaaggtgggg ctcgcgccgg gcgacccgct cgggcacccg
     3601 cgtcacctcg tcgggctggg gacgttccag ccggtccgcg agcttcttcg cctcgcgcgc
     3661 cgcctgcggc ccgagctgaa gaccgtcggc acggtctggt gcccttccga gagcagcgcc
     3721 gaggcgtgcg tgaagctcgc ccgggaagag tcggcggcgc tcggtctccg gctcctggag
     3781 gcgaacgccg acgcgccggc cgacgtcgcc gaggccgccc gatcgctcac ctcgcggggc
     3841 gtcgaggcgc tctggctcgg cggagacaac gtcgtcgaga cggccgtctc ctccctcgtg
     3901 aaggtcgccc gggaggcgaa ggtcccgctc ctggccaatg ccccgacgca cgcggaggcc
     3961 ggagcgctcg ccgggctcgg cgccgactac gtcgaggtcg gccggcgcgc cggagacctg
     4021 gcctcccgtc tccttgccgg cctcgacccg gcgacggtga gggtggagaa cgtcgccccg
     4081 cccctcctcg ccctgaacct ctcggcgctc tccggccttc gcgaccgctg gagcgtcccc
     4141 gaggatctcc ggtcggaggc ggccgtcgtg atcgacggga cgggcgtcaa gaccgagcgc
     4201 tccgccgccc ccgaggcagc cgtcacgccg accgcggccg gcgcgggccg acgacggatc
     4261 acgctcgtgc agctcgtctc cgggcccgcc atcgacgcga cgcgggaagg gatgctccgg
     4321 ggcctctccg acggaggatt cgtccccggg agggacgtcg acgtgaaggt ccgcgacgcg
     4381 cagggagagc tctccgccct ccccgctctc gtcgacgcgg cccgcgccga ccgcgctgac
     4441 ctcgtcctga cgatcacgac gcccgcgctc caggcggcga tggcacgcct gagcgaccgg
     4501 cccgtcgtct tcggcctctc gatcgacccg ctcctcgccg gggacggcgg gacccacgag
     4561 cggcaccggc cgaacgtcgc ggggatctac gaccggagcc cgttcgagcc gctcctcgac
     4621 ctcctccggc agctcctgcc gcgggcccgg aagatcggca ccctcttcaa ccccgccgag
     4681 ccgaactcgg tcctcttccg cgacgagctc gcccgcgtgg cccgctcccg gggcctcgcg
     4741 ctcgtcgccg tggcctccga ttctcccgcc ggcgtcgccg acgcggccct cgcgctcgtc
     4801 ggacgcgggg tcgacgccgt cttccaggtc aacgacaacc tccacgaggc gtccttctcc
     4861 gggatcgccg cggcggcccg gcgcgcccgc gtcccggttt tcgccttcag ctccccgctc
     4921 gtccacgaag gggcggcgat cgcgctcgcc aacgaccact ccgacggcgg gcgcgaagcc
     4981 ggccggatcg ccgcgagggt ccttcgcggc gagagcccgg cgtcgatccc ctaccgcggg
     5041 atcacgagga cggtcctcgc cgtgagcccc gaggccgcgg ctgcggccgg cctcgcgctc
     5101 ccggcggacc ttctctcccg cgcggccgtg gtcgccggtc gcccggcggc cgcagcgacg
     5161 ccgtccgtcg cgagccgggc cgccccgccc cccctcggcc gaacggcgaa cctgcaggtc
     5221 ctccacttcg ccgagtcgac gatggtcgag gaggcgatgg agggcctccg cgccgagctc
     5281 ccgcgcacgg ggctcgtcga ggggcgggac tacgagctga cggtgcgcaa cgcgcagggg
     5341 gagatgtcga acctcgtctc gcttctcgac gccgctctcg ccacgcgccc cgaggtcctc
     5401 ctcgtcacgt cgaccccgac gctccaggcc gcgctgaagc gcgtgaaggg ggtcccgatc
     5461 gtcttcagca tcgtcgccaa ccccgtcctg gccggcgccg gaacgagctt cgccgagcac
     5521 gccccgaacg tcaccggcat ctcgacggag agtgacttcg cggggatggc ccgggtcgtc
     5581 cgcgaatgcc tcccccgcgc ccgccgcgtc ggcaccctct tcaacccggg cgagacgaac
     5641 tcggtcttca accgggacgg gaccgccagg gcgctcgcgg ccgtcgggat cgagctcgtc
     5701 tcggtggcga cctcgacgcc ggccgaggtc ccggatgccg cgctcgcgct cgcggggcgc
     5761 ggtctcgacg cgatctgcca ggtgaacggc aacctgcacg attccggctt ctcggggatc
     5821 gcgcaggcgg cgcgaaaggc gaagctgccg ctcttcgcct tcacctccgc tcaggcgctc
     5881 tcgggaggcg ccgcggtggc ggtggcacgc gactacgtgc ag
//
LOCUS       DAOTEZ010000268         5896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_9667_length_5896_cov_5.488101, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000268 DAOTEZ010000000
VERSION     DAOTEZ010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9667_length_5896_cov_5.488101"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1297)
                     /locus_tag="P5164_13495"
     CDS             complement(<1..1297)
                     /locus_tag="P5164_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44952.1"
                     /translation="MTTAAPGPGVWKVTGLFALLAFLVVPVFPHFVSPNELSRWAAGA
                     SLVDRRSLAIAPDVAAHLGPRFEDVAERDGRLYPNKAPGATLAALPGYAAARPFAGPP
                     GPASMRIAVTAMRWSAATLPVLLLSLLFARAALARGAEPGACALSLLALLFGTPLFAY
                     GLLLFAHALVSLALFGAWALLYPPDQRESPRREVAAGALLGLAVLSDYPALVPVALLM
                     LAAAWRRPVARTVRICLGALPAAALFFGFNTIAFGGPFELPVSHERFAAFRSLREHGA
                     WGVGWPSVANLLRLLFDSSKGLFVLSPVLLAAAASTPAALRRLGLRAGATLVAVPVTL
                     FLVYSGFPDWHGGFTVGARYLVPALPFLLLPLAFVRWGPLATALAGASTAAVALTTLV
                     FPFVPPGFPLPWGSFAAPLLAKGLVAPNLLHLVSRPLAVV"
     gene            1310..2050
                     /locus_tag="P5164_13500"
     CDS             1310..2050
                     /locus_tag="P5164_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY44953.1"
                     /translation="MRHGKRVAVVMPAYNAEKTLERTFEEVPKERVDDIFLVDDASRD
                     GTVALARTIPGLTVVVHPENRGYGGNQKTCYATALASGADVVVMVHPDHQYDASVLHE
                     LIEPIAEGKVDAMFGSRMLGGRPLEGGMPKWKYLANVFLTAVENATFYLYLTEYHSGL
                     RAYSRRYLEAVNLAAFSDDFVFDTEIIAAGIWKGMRIREIPIVTRYFDEASQIDFVRS
                     CVYGFSILGVMVRYKLHKWGWRNDPIFK"
     gene            2047..4314
                     /locus_tag="P5164_13505"
     CDS             2047..4314
                     /locus_tag="P5164_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44954.1"
                     /translation="MRRLLPAAVAAIVLLAVGLANLDLLSGRATPSFRDLATTQRPAR
                     ALAASLGDAALNPHASFGQTFRGNPNLVLAYPFPAAPRWLGVHLLLHAALGGFGFLLF
                     LRLLGRSPEAALAGALAFSFSGFALSCTAFLNSATTLAWVPWLLASVAAARLPGRHAL
                     RLALLGSAASGALLAHAGEPALGLIGILLALAFALSGPRGTRLRASGALVGGGVFALL
                     ASAPYLLDVLRATETSARRLRGFSWTEFSAVGFHPLRLLETPFPFLFGDPSRVLSGAY
                     WGYAASQGNPPYLASLSFGVLPLALAAAFALCPRRSEGRFWLGAAGLALFVAALPWVP
                     GARAAYEALPFLHSVRYPVKALLVATIAVAVLAALAVERLLLEEALPRWRSRAGLVLL
                     VLAGSFGLAAVAFRARPELPRGLLLSLWDPAWASDPGVVLAPVLARLPQQAASSAALL
                     LVAGLLLARGLGDLRSRVLLLAALSAEGLAQARFHVPRAPAELYDRPAPLVAAAARIP
                     GRVFERAWKDIDAVRRGLAGPLIADERLGFALAQTTQGWSLAGAPHGLRYAWDADPDG
                     SYTLLDRYARDVVLSRAWPERLEWLRAAGVGAVIASDVPPGLAGLVPVYVEGRFGPPA
                     TLFRLAEPLPGVRRLSRVRGAASVDAAVAVVDAAEFDPATDGVVAGRPPAGTDAAEAD
                     PAASARVLAEGPDALVVETDGARPGLLLLDRSFTPAVRATANGRKTTVHAVQINLVGI
                     AVPPGPSRVEVRLAP"
     gene            complement(4621..5514)
                     /locus_tag="P5164_13510"
     CDS             complement(4621..5514)
                     /locus_tag="P5164_13510"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRY44955.1"
                     /translation="MRGTVEGVPRLLDLLARHGARATFLFSLGPDSTGKAVKRVFRRG
                     FVRKVMSASPGASYGLATMLYGTLLPAPDIGRRKEAVTRMREADAAGHECGIHGWDHV
                     DWHDRLLTMEREEVFATFRRAADRFADLLGRRASVAGAPGWAASGRSVEAYEAAGIRV
                     ASDTRGGPPFRPLRESGAPSGLVEVPSTLPTLDELLAWAAFRGAAREGTVEHLRRSVA
                     EAPGVSVHSIHTEIEGGRAFSDLFTRQLAAWAADGVAFVTLSEAAAAAGEPATLPARP
                     LRQVSVTGRALPVTTGAPPAG"
     gene            complement(5558..>5896)
                     /locus_tag="P5164_13515"
     CDS             complement(5558..>5896)
                     /locus_tag="P5164_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HRY44956.1"
                     /translation="LMKILVNENGCADGQIFNLGNPAADLSIRELAERLVAAVGTYEK
                     FAPLARAATLVEVSAAEYYGANYQDITTRVPSVRKAKECLGWTPTTDFATGLAKTLDF
                     YLSGRLRPEV"
BASE COUNT          762 a         2232 c         2096 g          806 t
ORIGIN      
        1 cgacgaccgc gagcggccga gagacgaggt ggaggaggtt cggggcgacg agccccttcg
       61 cgaggagcgg cgccgcgaaa ctcccccatg ggagcgggaa tccgggcggc acgaacggga
      121 agacgagcgt cgtgagcgcg acggccgccg tggacgcgcc ggcgagagcc gtggcgagag
      181 ggccccagcg gacgaacgcg agaggcagga ggaggaacgg cagcgccggg acgaggtagc
      241 gagcgccgac ggtgaagccg ccgtgccagt cggggaagcc ggagtaaacg aggaacaggg
      301 tgaccgggac ggcgacgaga gtggcgccgg cccgcaggcc gaggcggcgg agcgccgcgg
      361 gcgtcgacgc ggccgcagcc aagaggacgg gcgataggac gaagagtccc ttcgaggaat
      421 cgaagaggag ccggaggagg ttcgcgaccg agggccagcc gaccccccag gcgccgtgct
      481 cccgaagcga gcggaacgcc gcgaatcgct cgtggctcac cgggagctcg aagggtccgc
      541 cgaacgcgat ggtgttgaag ccgaagaaga gcgccgcggc agggagggcg ccgagacaaa
      601 ttcgtaccgt ccgcgcgaca gggcgccgcc aggccgccgc gagcatcaga agtgcgaccg
      661 ggacgagtgc cgggtagtcc gagaggacgg ccagcccgag gagcgcaccg gccgccacct
      721 cgcgcctggg gctctcccgc tggtcgggcg ggtagaggag ggcccatgcg ccgaacaggg
      781 cgagcgagac gagggcgtgg gcgaagagga ggaggccgta ggcaaagaga ggcgtcccga
      841 agaggagtgc gagaagggag agggcgcaag cgccgggctc ggctccccgt gcgagagctg
      901 cgcgcgcaaa gagaagcgag aggaggagga ccggaagcgt cgcggcgctc cagcgcatcg
      961 ccgtgacggc gatacgcatc gaggccggcc cgggcggccc ggcgaagggc cgcgccgcgg
     1021 cgtagcccgg aagtgccgcc agagtcgccc cgggcgcctt gttcggatag aggcggccgt
     1081 ccctctccgc gacgtcctcg aagcgcggcc cgaggtgggc cgcgacgtcg ggcgcgatcg
     1141 cgaggctccg ccggtcgacg agggacgcgc ccgccgccca ccgcgaaagc tcgttcggcg
     1201 agacgaagtg ggggaagacc ggcacgacga ggaaggcgag gagggcgaaa aggcccgtca
     1261 ccttccacac cccgggcccc ggcgccgcag tcgtcataat ctccggatca tgcgccatgg
     1321 gaagcgggtc gcggtcgtca tgccggccta caacgcggag aagaccctcg aacggacctt
     1381 cgaggaggtc ccgaaggaac gggtcgacga catcttcctc gtcgacgacg cctcgcggga
     1441 cggcacggtc gccctcgcgc ggacgattcc cggcctcacg gtcgtcgtcc accccgagaa
     1501 ccgtggttac ggcgggaatc agaagacgtg ctacgcgacg gccctcgcga gcggagccga
     1561 cgtcgtcgtc atggtccacc cggaccacca gtacgacgcc tcggtcctcc acgagctgat
     1621 cgagccgatc gccgagggga aggtcgacgc gatgttcggg agccggatgc tcggcggccg
     1681 cccgctcgag ggggggatgc cgaagtggaa gtacctcgcg aacgtcttcc tcacggcggt
     1741 cgagaacgcc acgttctacc tctacctgac ggagtaccac tcgggcctcc gcgcctactc
     1801 gcgccgctac ctcgaggcgg tgaacctcgc ggccttctcc gacgacttcg tcttcgacac
     1861 cgagatcatc gcggcgggga tctggaaggg gatgcggatc cgtgagatcc cgatcgtgac
     1921 ccgctacttc gacgaggcct cgcagatcga cttcgtgagg tcgtgcgtct acggcttctc
     1981 gatcctcggc gtgatggtcc gctacaagct ccacaagtgg ggctggcgga acgacccgat
     2041 cttcaagtga ggcgactcct gccggcggcc gtcgccgcga tcgtcctcct cgccgtcggc
     2101 ctcgcgaacc tcgacctcct ctccggccgt gcgacgccct cgttccggga cctcgcgacg
     2161 acccagcgcc ccgcccgcgc gctcgccgcc tcgctcggcg acgccgcgct gaacccgcac
     2221 gcctcgttcg gccagacctt tcgcggcaac ccgaacctcg tcctcgccta cccgttcccc
     2281 gccgcccccc ggtggctcgg cgtccacctc ctgctccacg cggcgctcgg cgggttcggc
     2341 ttcctcctct tcctccgcct cctcggtcgc tcacccgagg cggcgctcgc gggagcgctc
     2401 gccttctcct tctccggctt cgccctctcc tgcaccgcct tcctgaactc ggcgacgacg
     2461 ctcgcctggg tcccgtggct cctcgcctcg gtcgccgcgg cccggctccc ggggcgtcac
     2521 gcgctccgcc tcgccctcct cggctcggcc gcctcagggg ccctcctcgc gcacgcgggc
     2581 gagcccgccc tcggcctgat cgggatcctc ctcgcgctcg ctttcgcgct ctccggaccc
     2641 cgagggacac gtctccgcgc gtccggcgcg ctcgtcggcg gaggcgtctt cgccctcctc
     2701 gcctccgccc cctacctcct cgacgtcctc cgcgcgacgg agacctccgc ccgacgcctc
     2761 cgcggcttct cgtggacgga gttctcggcg gtcggcttcc acccgctccg gctcctcgag
     2821 acccccttcc cgttcctctt cggcgacccg tcgcgggtcc tctcgggcgc ctactggggc
     2881 tacgccgcgt cgcaggggaa cccgccctac ctcgcctcgc tctcgttcgg tgtcctcccg
     2941 ctcgccctcg cggccgcgtt cgcgctctgc ccgcgccgaa gtgagggtcg cttctggctc
     3001 ggggccgccg gcctcgcgct cttcgtcgcg gctctcccct gggtcccggg agcgcgtgcg
     3061 gcgtacgagg cgctcccgtt cctccattcc gtccgttacc cggtcaaggc gcttctcgtc
     3121 gcgacgatcg ccgtcgcggt cctcgccgcg ctcgcggtcg aaaggctcct cctcgaggag
     3181 gcgctgcccc gctggcgctc ccgcgccggc ctcgtcctcc tcgtcctcgc cggctccttc
     3241 ggccttgcgg cggttgcctt tcgcgcacgc ccggagctcc cgcgcgggct cctcctctcc
     3301 ctctgggacc ccgcgtgggc ctccgacccc ggcgtcgtcc tcgccccggt cctcgcgcgc
     3361 ctcccgcagc aggcggcctc ctccgcggcg ctcctcctcg tcgcggggct cctcctcgcg
     3421 cgcgggctcg gggacctccg aagccgcgtc ctcctcctcg ccgccctctc cgccgagggg
     3481 ctcgcgcagg cccgtttcca cgtcccgcgc gcccccgcgg agctctatga ccgccccgcg
     3541 cccctcgtcg ccgcggcggc gcggatcccg ggccgcgtct tcgagcgggc ctggaaggac
     3601 atcgacgcgg tgaggcgcgg cctggcaggt ccgctcattg ccgacgagcg gctcggcttc
     3661 gcgctcgcgc agacgacgca agggtggtcg ctcgcgggcg cgccgcacgg cctccgctac
     3721 gcctgggacg ccgaccccga cgggagctac accctcctcg accgctatgc ccgggacgtc
     3781 gtcctctccc gcgcctggcc cgaacggctc gagtggcttc gcgcggcggg cgtcggcgcc
     3841 gtcatcgcga gcgacgtccc ccccggcctc gccggcctcg tccccgttta cgtcgagggc
     3901 cgtttcggtc cgccggcgac tctcttccgg ctcgcggagc cgctgccggg cgtccgccgc
     3961 ctctcgcggg tccggggggc cgcctccgtg gatgctgccg tcgccgtcgt cgacgcggcc
     4021 gagttcgacc cggccacgga cggcgtcgtc gccgggcgcc ctcccgcggg gaccgacgcc
     4081 gccgaggcgg atccggccgc ctccgcgcgg gtcctcgccg agggtcccga cgctctcgtc
     4141 gtcgagacgg acggggcgcg tcccggcctc ctcctcctcg accgctcgtt cacccccgcc
     4201 gtccgcgcga cggcgaacgg gcggaagacg accgtccacg cggtccagat caacctcgtc
     4261 ggaatcgccg ttccccccgg cccctcccgc gtcgaggtcc gcctcgcccc ctgagcgttc
     4321 cgggagcggt gccccgccgc tccccccggg gatggtgctc cgcggacggt ggtcccccgg
     4381 ggatggatgc cccgtggagg tgccccccgg gccaggtctg gtgccactgg tctggtgcca
     4441 ggcgtgaaat cgtgtagttt ttctggtctg gtgccaggcg tgaaatcgtg tagttttcaa
     4501 aaactacacg atttcacgcc tggcacccaa cttgtggcac ccaacttgag ggcaccccgg
     4561 gtgaaggcac cgagggcgga cggcaccgtg ggcggacacc gaaggcgttc tggcgccggt
     4621 tcagccggcg ggcggcgccc cggtggtgac cggcagcgcc ctccccgtca ccgagacctg
     4681 ccgcaggggc cgggcgggaa gcgtcgccgg ctcgccggcg gcggccgccg cctccgagag
     4741 cgtcacgaag gcgacgccgt cggcggccca ggccgcgagc tgacgcgtga agaggtccga
     4801 gaacgcgcgt cccccctcga tctccgtgtg gatcgagtgg accgagaccc cgggcgcctc
     4861 cgcgaccgaa cgtcgaaggt gctccaccgt tccctcccgg gccgcgccgc ggaaagcggc
     4921 ccaggccagg agctcgtcga gcgtcgggag cgtcgagggg acctcgacga gtcccgaggg
     4981 cgcgccgctt tcgcggagcg gccgaaatgg cgggccgcca cgcgtgtcgg aggcgacgcg
     5041 gatgccggcc gcctcgtagg cctcgacgga gcgcccgctc gcggcccagc ccggcgcgcc
     5101 ggcgacactc gcccggcgcc cgaggaggtc ggcgaagcgg tccgcggcgc gccggaacgt
     5161 ggcgaagacc tcctcgcgct ccatcgtcag cagccggtcg tgccagtcga cgtggtccca
     5221 gccgtggatg ccgcactcgt gcccggccgc gtcggcctcg cgcatcctcg tgacggcttc
     5281 cttccgccgc ccgatgtcgg gggcggggag gagggtcccg tagagcatcg tcgccaggcc
     5341 gtacgaggcg cccggcgagg cgctcatcac cttcctcacg aagccgcggc ggaagacgcg
     5401 tttcacggcc ttcccggtcg agtcgggccc gagcgagaag aggaacgtgg cccgggcgcc
     5461 gtgccgcgcg aggaggtcga ggagacgggg gaccccctcg accgtcccgc gcaacgtgtc
     5521 gacgtcgacc ttgagggcga ggcgcgaagc cgggacgcta cacctcgggt cgcagccggc
     5581 cggagaggta gaagtcgagc gtcttcgcga ggccggtcgc gaagtcggtc gtcggcgtcc
     5641 acccgaggca ctccttcgcc ttccgcaccg aggggacgcg cgtcgtgatg tcctggtagt
     5701 tcgcgccgta gtactccgcc gccgagacct cgacgagcgt cgcggcccgc gcgagcgggg
     5761 cgaacttctc gtacgtcccg acggcggcga cgagccgctc ggcgagctcc cggatcgaga
     5821 ggtcggcggc ggggttgccg aggttgaaga tctggccgtc ggcgcagccg ttctcgttga
     5881 cgaggatctt catgag
//
LOCUS       DAOTEZ010000269         5880 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_9709_length_5880_cov_7.040687, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000269 DAOTEZ010000000
VERSION     DAOTEZ010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9709_length_5880_cov_7.040687"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="P5164_13520"
     CDS             complement(<1..899)
                     /locus_tag="P5164_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44957.1"
                     /translation="MNGPLAEYAGFAALWKRFAPETPFGRAARAAAGVLTDRAELEER
                     WDLTDEALRLLDALEGDRVRLDRIRHHLRRIPRFPESPREAFDEVELFQLKKLLANHR
                     RLLALLPDGAKARFGLAPLPESLHALLGTGREGDESFHVADAYDPELASVRSEIRRAD
                     AEAAALRASRDEALQARCGFALGDRPFLLVPREVLGEPAGVSDLLEIEAWDATRLCVR
                     PRPSVAELRLGEEKAALLARERGIEARVLRALSKPLSEALPLFLEQAGAVERLDLALA
                     GARLAREAGLVRPILHDGPVRIAG"
     gene            complement(896..1753)
                     /locus_tag="P5164_13525"
     CDS             complement(896..1753)
                     /locus_tag="P5164_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44958.1"
                     /translation="MPAGLETIPGGVTAVTGFEKGSGKTTLLGALRPLARRAGPVALF
                     GIGVDGSLKGGGDGRAAVVRVEAGDVVQTTDLFARASSTRLEVLDVLPSRTSLGRLLL
                     GRAIRDGEVTLVGDESLSALSRSIDLVRDEEWAASVLVDGAASRITQAAALDGVRFLF
                     TVRVDGANLSRVAERVRALAALATLPVVPDPPEGTLRLEGPLTEEGREALPRDLGALS
                     LEDLTKSFLPPRLLLRLLETVACSVRRGIALLGVVASRRDVTEEELREAVGPLGAPFL
                     LPSPFEAAA"
     gene            complement(1820..3088)
                     /gene="ablA"
                     /locus_tag="P5164_13530"
     CDS             complement(1820..3088)
                     /gene="ablA"
                     /locus_tag="P5164_13530"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005979937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HRY44959.1"
                     /translation="MQYPPVDFRSIPLFANVTDAEWNDWSWQIRNGIRDVETLEKVVK
                     LTDREREEIRQCLGKFTMEITPYYAALMDRDDPFCPVRMQSVPRIAEMHDDPSDMADP
                     LHEDVDSPVPGLTHRYPDRVLLLVTNICSMNCRHCTRRRLVGDTDVDMPEDNVSAAIE
                     YIRSTPTVRDVLLSGGDPFVLPDARLRSILERLRAIPHVEIIRIGTRTPVVLPQRITD
                     DLVGMLKEFHPLWVNVHFNHPKEITAEAKAACERLANAGIPLGNQSVLLRGINDDPIV
                     MKKLVHKLMTVRVRPYYIYQCDLSLGISHFRTSVAKGIEIIESLRGHTTGLAVPTFVV
                     DAPGGGGKIPVMPNYLISMAEGKAILRNYEGVITSYSWPRGRGPANPATKDPGDDAKY
                     RAKDGVATLLSGDRRNLEPSGLARHKRNND"
     gene            complement(3099..3923)
                     /locus_tag="P5164_13535"
     CDS             complement(3099..3923)
                     /locus_tag="P5164_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HRY44960.1"
                     /translation="MSDKVIITCAVTGAETTRESQPALPITPEEIAASAFEAWEAGAA
                     VLHLHVRHDDGSPTQDVAVFRKAIELVRAKCDIVIECTTGGAVGMTPEERLQPVTLQP
                     EMASLDCGTVNFGDDYIVNTLPIMREFAKSMRAHGVRPTLECFDLGHVYASHILIKEG
                     LLDEPYHYGLVLNVPGSVKYEPDVLEIFVRKLPRGAHWTLMGIGGKANLDAIYGALAL
                     GGNVRVGFEDNIYYSKGRLAKSNAELVERAARIAKDCGRPLARPDDVREMLKLRKA"
     gene            complement(3958..4989)
                     /locus_tag="P5164_13540"
     CDS             complement(3958..4989)
                     /locus_tag="P5164_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HRY44961.1"
                     /translation="MACKYGSHRVLEPKGVLPQPALRLDNDFSTIADNEVLVDVKALN
                     IDSASFTQIEEEAGGDLAKIEQKILSIVAERGKMQNPVTGSGGMFIGSVAKVGAALAG
                     RDLAPGEKVASLVSLSLTPLKIERIVKIHPEIDRVEIVGQAVLFESGIYAKLPSDMDE
                     TLALAALDVAGAPAQAAKLVRPGQSVLILGAGGKSGMLVAWEAMKRVGPTGRVVGNVY
                     LAEDAETLRSLGLCHEVVVADATKPVALTEAVLAANGGAEYDVVFNCVNVQNTEMSSI
                     LPCRQEGTVYFFSMATHFGKAALGAEGVGKDVTMIVGNGYTKGHAEITLAELRENAKL
                     RRHFETRYV"
     gene            5134..>5880
                     /locus_tag="P5164_13545"
     CDS             5134..>5880
                     /locus_tag="P5164_13545"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HRY44962.1"
                     /translation="MTLRTAVRLTRPFTLLPPLLGIVSGAVCAFGSVHNPDPEHRLTW
                     AVVAAIALGSLCASFLNAASNVLNQITDLEVDRVNKPDRPLVTGEAGLRETWVLTWVL
                     YALAMLPVWFVVVPPREGIAARLLAPLGTHQTFFLFLGGLVFTLVYSLPSWGRTKRLG
                     MWANVTIAVPRGCLLKVAGWTMVAPANALEPWVIGFLFFLFLVGAASTKDFSDVPGDR
                     AGGCRTLPILYGNRKAVAIMSPFFVLPWLLL"
BASE COUNT          759 a         2082 c         2125 g          914 t
ORIGIN      
        1 ccggcgatcc ggacgggccc gtcgtggagg atcggccgca cgagacccgc ctcgcgggcg
       61 aggcgcgcgc ccgcgagcgc gaggtcgagc cgctcgacgg cgcccgcctg ctcgaggaag
      121 agcgggagag cctccgagag cggcttcgag agcgcgcgga ggacgcgcgc ctcgatcccg
      181 cgctcgcggg cgaggagggc ggccttctcc tcgccgagcc ggagctccgc gacgctcggc
      241 cggggccgca cgcagaggcg cgtggcgtcc cacgcctcga tctcgaggag gtccgagacg
      301 ccggccggct cgccgaggac ctcgcgcggg acgaggagga acgggcggtc gccgagggcg
      361 aatccgcagc gagcttgaag cgcctcgtcg cgcgaggcgc ggagcgcggc ggcctcggcg
      421 tcggcgcggc ggatctcgct gcggacggag gcgagctccg ggtcgtacgc gtcggctacg
      481 tggaacgact cgtccccctc ccgccccgtt ccgaggaggg cgtgcagcga ttcggggagc
      541 ggggcgaggc cgaaccgggc cttcgccccg tccggcagga gggccaggag ccgccggtgg
      601 ttcgcgagga gcttcttcag ctggaagagc tccacctcgt cgaacgcctc gcgcggcgac
      661 tcggggaacc gggggatgcg gcggaggtgg tggcggatcc ggtccagccg gacgcggtcg
      721 ccctcgaggg cgtcgaggag ccggagcgcc tcgtcggtga ggtcccagcg ctcctcgagc
      781 tcggcccggt cggtgaggac tcccgccgcc gcgcgggcgg cccgcccgaa cggggtctcc
      841 ggcgcgaagc gcttccagag cgctgcgaag ccggcgtact ccgcgagcgg cccgttcatg
      901 ccgccgcctc gaacgggctc gggaggagga acggcgcgcc cagcggaccg accgcctcgc
      961 ggagctcctc ctccgtcacg tcgcgccgcg aggcgacgac gccgagaagc gcgatcccgc
     1021 gtcggacgct gcacgccacc gtctcgagga ggcggaggag gaggcgcggc ggcaggaacg
     1081 acttcgtgag gtcttccagg gagagcgccc cgaggtcgcg cgggagggcc tcgcggccct
     1141 cctccgtgag cggcccctcg aggcggagcg ttccctcggg agggtccggg acgaccggaa
     1201 gcgtcgcgag ggcggcgagg gcccggaccc gctcggcgac gcgggagagg ttcgcgccgt
     1261 cgacgcggac ggtgaagagg aagcggacgc cgtcgagcgc ggcggcctgc gtgatccggc
     1321 tcgcggcgcc gtccacgagg accgacgcgg cccactcctc gtcccgcacg aggtcgatcg
     1381 agcgggagag cgccgagagg ctctcgtctc cgacgagcgt cacctcgccg tcgcggatcg
     1441 cccggccgag gaggagccgc ccgagcgacg tgcgcgaagg gaggacgtcg aggacctcga
     1501 gccgggtgga cgacgcccgg gcgaagaggt cggtcgtctg gacgacgtcg cccgcctcga
     1561 cgcggacgac ggccgcgcgc ccgtccccgc ctcccttcag gctcccgtcg acgccgatgc
     1621 cgaagagcgc gacggggccg gcgcgtcgcg cgagggggcg cagggccccg aggagggtcg
     1681 tctttcccga gcccttctcg aagccggtca ccgcggtgac ccctccgggg atcgtctcga
     1741 gcccggccgg cacggcctca acgggacgcc gcggatcgcg gcgtcccgcc cccgttcccc
     1801 gcggcgccag cgggtccggt cagtcgttgt tcctcttgtg ccgcgcgagg ccgctcggct
     1861 cgaggttgcg ccggtcgccg gagaggagcg tcgcgacgcc gtccttcgcg cggtacttcg
     1921 cgtcgtcgcc ggggtccttc gtggccgggt tcgccggccc tcggccgcgg ggccacgagt
     1981 aggacgtgat gaccccttcg tagttgcgga ggatcgcctt cccctcggcc atcgagatga
     2041 ggtagttcgg catcacgggg atcttcccgc cgccccccgg cgcgtcgacg acgaacgtcg
     2101 ggacggcgag gccggtcgtg tggccgcgga gcgactcgat gatctcgatc cccttcgcga
     2161 ccgacgtccg gaagtggctg atcccgagcg acaggtcgca ctggtagatg tagtagggcc
     2221 tcaccctcac cgtcatcagc ttgtggacga gcttcttcat gacgatcggg tcgtcgttga
     2281 tcccgcgcag gaggacgctc tggttcccga gcgggatgcc ggcgttcgcg agcctctcgc
     2341 aggccgcctt cgcctccgcg gtgatctcct tcgggtggtt gaagtggacg ttcacccaga
     2401 gcgggtggaa ctccttcagc atcccgacga ggtcgtcggt gatccgctgc ggcaggacga
     2461 ccggcgtgcg cgtgccgatc cggatgatct cgacgtgggg gatcgcccgc agccgctcga
     2521 ggatcgaacg gagccgcgcg tcggggagga cgaacgggtc gccgcccgag aggaggacgt
     2581 ccctgaccgt cggggtgctg cggatgtact cgatcgccgc ggagacgttg tcctccggca
     2641 tgtcgacgtc ggtgtcgccc acgaggcgcc gccgcgtgca gtgccggcag ttcatcgagc
     2701 agatgttcgt gacgaggagg aggacccggt cggggtagcg gtgggtcagt cccgggacgg
     2761 ggctgtcgac gtcctcgtgg agcgggtcgg ccatgtcgga cgggtcgtcg tgcatctcgg
     2821 cgatgcgcgg caccgactgc atccgcacgg ggcagaacgg gtcgtcccgg tccatcagcg
     2881 ccgcgtagta cggcgtgatc tccatcgtga acttcccgag gcactgccgg atctcctcgc
     2941 gctcgcggtc ggtcagcttc acgaccttct cgagcgtctc gacgtcgcgg atgccgttcc
     3001 ggatctgcca ggaccagtcg ttccactccg cgtccgtcac gttcgcgaag agggggatcg
     3061 accggaagtc gacggggggg tactgcatgg tctcttcctc aagccttccg gagcttcagc
     3121 atctcgcgga cgtcgtcggg gcgggcgagg gggcgcccgc agtccttcgc gatgcgggcg
     3181 gcgcgctcga cgagctcggc gttgctcttc gcgaggcgcc ccttcgagta gtagatgttg
     3241 tcctcgaagc cgacgcggac gttcccgccg agggcgagcg cgccgtagat cgcgtcgagg
     3301 ttcgccttcc cgccgatccc catgagggtc cagtgcgccc ccctcgggag cttcctcacg
     3361 aagatctcga ggacgtccgg ctcgtacttc accgacccgg ggacgttcag gacgaggccg
     3421 tagtggtacg gctcgtcgag gagcccttcc ttgatcagga tgtggctcgc gtagacgtgc
     3481 ccgaggtcga agcactcgag cgtcggccgc accccgtgcg cgcgcatcga cttcgcgaac
     3541 tcccgcatga tcggcagcgt gttgacgatg tagtcgtcgc cgaagttgac cgtgccgcag
     3601 tcgaggctcg ccatctcggg ctggagcgtc accggctgga ggcgctcctc gggcgtcatc
     3661 ccgacggcgc cgcccgtcgt gcactcgatc acgatgtcgc acttggctcg gaccagctcg
     3721 atcgccttcc ggaagacggc cacgtcctgc gtcggcgatc cgtcgtcgtg gcggacgtgg
     3781 aggtggagga cggcggcgcc ggcctcccac gcctcgaacg cgctcgcggc gatctcctcg
     3841 ggggtgatcg ggagggcggg ctgcgactcg cgcgtcgtct cggcgccggt gacggcgcag
     3901 gtgatgatga ccttgtcgct catgtgagcc tccggggagc gcgccggacg ggcgctctca
     3961 gacgtagcgg gtctcgaaat ggcgccggag cttcgcgttc tcgcggagct cggcgagagt
     4021 gatctcggcg tggcccttcg tgtagccgtt gccgacgatc atcgtgacgt ccttgccgac
     4081 cccctcggcg ccgagcgcgg ccttgccgaa atgggtcgcc atgctgaaga agtagaccgt
     4141 cccttcctgc cggcagggca ggatgctgct catctcggtg ttctgcacgt tcacgcagtt
     4201 gaagacgacg tcgtactcgg cgccgccgtt cgccgcgagg acggcctccg tcagcgcgac
     4261 cggcttcgtc gcgtcggcga cgacgacctc gtggcagagg ccgagcgacc ggagcgtctc
     4321 ggcgtcctcg gcgaggtaga cgttcccgac gacgcggccc gtcggcccga ccctcttcat
     4381 cgcctcccag gcgacgagca tgccgctctt tccgccggcg ccgaggatca ggacgctctg
     4441 cccgggcctc acgagcttgg cggcctgggc gggcgcgccg gcgacgtcga gcgccgcgag
     4501 cgcgagcgtc tcgtccatgt ccgaaggcag cttcgcgtag atgccgctct cgaacaggac
     4561 ggcctgcccg acgatctcga cccggtcgat ctccgggtgg atcttcacga tcctctcgat
     4621 cttcagcggc gtcaggctga gcgagaccag gctcgcgacc ttctcgcccg gcgcgaggtc
     4681 ccgcccggcg agggcggcgc cgaccttcgc gacgctgccg atgaacatcc cgcccgagcc
     4741 ggtgacgggg ttctgcatct ttccccgctc ggcgacgatc gagaggatct tctgctcgat
     4801 cttcgcgagg tcgccgcccg cctcctcctc gatctgcgtg aagctcgcgg agtcgatgtt
     4861 gagcgccttc acgtcgacga ggacctcgtt gtcggcgatc gtcgaaaagt cgttgtcgag
     4921 cctcagggca ggctgcggga ggacgccctt cggctcgagg acgcggtggg agccgtactt
     4981 gcaggccatg gaaccctctt tgcgaaagcg tctcggggtc tcgcccggac cggcgcgccc
     5041 gacgcgttcg cgccctgcgg gcgccgcttc cgcggccccg gggccgaccc gagagcatat
     5101 cagggctccg gtgtaccctc gatgaccgcg atcatgaccc tccgcaccgc cgtccgcctc
     5161 acccgcccct tcaccctcct ccccccgctc ctcggcatcg tctcgggcgc ggtctgcgcc
     5221 ttcgggagcg tccacaaccc cgacccggag caccggctga cctgggccgt cgtcgcggcg
     5281 atcgccctgg ggtcgctctg cgcctcgttc ctgaacgccg cctcgaacgt cctgaaccag
     5341 atcaccgacc tcgaggtgga ccgggtcaac aagcccgacc ggccgctcgt caccggcgag
     5401 gcggggctcc gcgaaacgtg ggtcctgacg tgggtcctct acgcgctcgc catgctcccg
     5461 gtctggttcg tcgtcgtccc gccgcgcgag gggatcgcgg cgcgcctcct cgccccgctc
     5521 gggacgcacc agacgttctt cctcttcctc ggcggcctcg tcttcaccct cgtctattcg
     5581 ctcccgtcct gggggcggac gaagcgcctc ggcatgtggg cgaacgtgac gatcgcggtc
     5641 ccgcgcgggt gcctcctgaa ggtggccggc tggacgatgg tcgccccggc gaacgccctc
     5701 gagccgtggg tcatcggctt cctcttcttc ctcttcctcg tcggcgccgc gtccacgaaa
     5761 gacttctccg acgtccccgg cgatcgcgcg gggggctgcc ggacgcttcc gatcctctac
     5821 gggaaccgga aggccgtggc gatcatgtcc cccttcttcg tcctcccgtg gctcctcctg
//
LOCUS       DAOTEZ010000270         5880 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_9710_length_5880_cov_6.003948, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000270 DAOTEZ010000000
VERSION     DAOTEZ010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9710_length_5880_cov_6.003948"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="P5164_13550"
     CDS             <1..927
                     /locus_tag="P5164_13550"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRY44963.1"
                     /translation="DPVFSPDGARLAVVLSGPAAPADVWLLERARGTLRQLTRSPRAG
                     MDSESLARPTLVRLSGRDGLPIPARLYRPRGASGPGPLVLSLHGGPEASGPQAFPADV
                     QALVAEGISVLVPSVRGSTGSGRRFASLDDGPLRRDAIEDVAACVDWAVSSGLADPKR
                     VGVLGVSGGGYLAMAALAAFPDRFAAAATLAGFADLPSFLRSTEPWLAAASKAEYGDP
                     ETDPLLLATLSPARRLEHVTTPTLVLHGANDTICPVGDAERLVRSLDRRGVPTRWVVF
                     PDEGHGFRKTSSRVRATVEVVRWFATYLAGPE"
     gene            complement(958..2376)
                     /locus_tag="P5164_13555"
     CDS             complement(958..2376)
                     /locus_tag="P5164_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRY44964.1"
                     /translation="MTVAAIREGFETADREKRFVALSSVLAAVFLTGMKVAVGVSTGS
                     IGILAEAAHSSLDLVAAVVTLWAVRAAGRPADPEHTYGHGKIENLSALFETALLLLTC
                     LWIVYEAIERLFFHPAHVEPTAWAFAVMAISIVVDVSRSRALARTAKKYGSRALEADA
                     LHFSTDVWSSAVVLLGLALVVVSQRTGVAWLAKADAVAALVVAAIVVALSLNLGKKSI
                     DDLVDAVPVGLRERLVAAARVPGVVDVPALRVRRTGPHAFVDATLTVGRHLALERAHE
                     ISDAAEAALKGLLPGVDVVAHVEPVSEGSEGLLATTRLLAARHGLGAHAIRTHATDGS
                     VSMELHLEVDERLTVREAHAVVSEFEENLRRAAPEVAHVVSHIEPVGETTAARRTTPA
                     DDAPVRSELERLRIERGPSFRPEGLAVRRGEDGLFVSFSLPVPADLPVGEAHALSDEV
                     ERSLKRRLGGGRVVIHVEPREV"
     gene            complement(2373..3014)
                     /locus_tag="P5164_13560"
     CDS             complement(2373..3014)
                     /locus_tag="P5164_13560"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRY44965.1"
                     /translation="MSRSLRRLGGLSAVLLLLLVAAVFAMSALRVGFSGTEAETAAAR
                     AAAEAAGVPLDSWKTLVVASSGSVGAPAAKAWEVFSRLEEWPLWSAPLHLGARWTGEP
                     GFRVAGGFEQVLALGFPLGTVRAREFVTRLTPGREVTWCRDEAGIVSCHVWRFTPVTP
                     TRTFVVNVEVLHGRPVGLFAPLVQSRWSRLFQGAVSGLARRAEGEAERAAAAP"
     gene            complement(3025..3918)
                     /locus_tag="P5164_13565"
     CDS             complement(3025..3918)
                     /locus_tag="P5164_13565"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY44966.1"
                     /translation="MALSAFFFAVMAAGAKLLGGRVPPQEVILVRGVLNALFMLWLLR
                     RRGLSWKPKRPGLLVLRGALGHFALSCYFWSVSRQPLATAVLLQQVHPVFTAVLAAWF
                     LSEKPGKRFVPAFLLAASGVALLVPRGAAEAGAASSSVAPLVVGLLGALLSAAAYVTV
                     RDASRTEHEETIVLWFPLTSIPLATVGTLVEGPAIPTPAEWGWLVFIAAAGQLGQLFL
                     TRGLARVPAGRATLANPLTVVFGALLGWIAFAEPLSLATLAGGAAIVAAVLLAGSGKP
                     SGADAAREAPPSPGPPPSPGA"
     gene            3987..4865
                     /locus_tag="P5164_13570"
     CDS             3987..4865
                     /locus_tag="P5164_13570"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY44967.1"
                     /translation="MAHLVLALTVLVWSFSFLAAARLRQELGLGEALAARFVPVLLGA
                     GAYLATRRPLRIPRAAWPTVVAMGLLSVPVYNVFFFHGMKTVPSGTAALIIALNPVFT
                     AVLARVVLGEPFGRRHVAGLALALAGVFVVIRYGTDKSVDWPYLSSALLLALAPLSWA
                     FYAVLGRRLPAEADPIDTSYALLFAGSLPLLAFARPSTAAKLASSPGALGAALYLAVP
                     CTLLAWVSFLWALKRLPAGEAAAFIFLNPPLANLWAFVLEGQPLRPPFLAGAAILLAG
                     VGLIVLRPGGRSRLPA"
     gene            4862..5788
                     /locus_tag="P5164_13575"
     CDS             4862..5788
                     /locus_tag="P5164_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRY44968.1"
                     /translation="MSGPSHAPGGGAQRYALLSIAAALATISLKTVAWKVTGSVGLLS
                     DAVESVVNLLAAVVALWALRFAAAPPDAEHQFGHAKAEYLASAFEGLAILGASVAIAL
                     TAWERIADPRPIEQAWLGIGVSTVAAAVNGGVAFVLFRAGRRLDSIALRSDSHHLMTD
                     VWTSAGVLAGVALVELTGWHLLDPIVALGVATNIVWMAGRILLETAQGLLDRTIPETE
                     KALLEALLARYRDNGVELHAVRTRQSGPVRFVDMHVLVPGQWTVQRGHDLCEEIEREV
                     RALLPRASVLTHLEPVDDPVAWHEPGSPPAPR"
BASE COUNT          776 a         2227 c         2122 g          755 t
ORIGIN      
        1 gatcccgtct tctccccgga cggcgcacgg ctcgccgtcg tcctctccgg tcccgccgcg
       61 ccggccgacg tctggctcct cgagagggcg cggggaacgc ttcgccagct gacgagaagc
      121 ccccgcgccg gcatggactc ggagtcgctc gcgaggccga ccctcgtccg gctttcgggc
      181 cgggacggcc tcccgatccc ggcccgcctc tatcggccgc ggggagcctc cggaccgggg
      241 ccgctcgtcc tgtcgcttca cggcggtccg gaggcctccg ggccgcaggc cttccccgcc
      301 gacgtccagg ccctcgtcgc cgaggggatc tccgtactcg ttccgagcgt tcgtggaagc
      361 acgggctccg gccggcgatt cgcgagcctc gacgacggcc ctcttcggcg cgacgcgatc
      421 gaggacgtcg ccgcgtgcgt ggactgggcc gtctcctcgg gcctggccga cccgaagagg
      481 gtcggggtcc tcggggtctc cggcggcggc tacctggcga tggccgccct cgccgcgttt
      541 cccgatcgct ttgccgccgc cgccaccctc gccggtttcg ccgacctccc ctccttcctg
      601 cggagcaccg agccatggct cgcggcggcc tcgaaggcgg agtacggcga cccggagacg
      661 gaccccctgc tcctcgccac gctctcgccg gcccgccgac tcgagcacgt cacgacgccg
      721 acgctcgtcc tgcacggcgc gaacgacacg atctgccccg tcggagatgc ggagcgcctc
      781 gtccggagcc tggaccgtcg cggcgtgccg acgcgctggg tcgtctttcc cgacgaggga
      841 cacggctttc ggaagacctc gagccgcgtc cgcgcgacgg tcgaggtcgt ccgctggttc
      901 gcgacctacc tggccggtcc ggagtagccg gccggccccg ccggccgcgc cgccggctca
      961 gacctcccgc ggctcgacgt ggatgacgac ccggcctccg ccgagccggc gcttcaagct
     1021 ccgctcgacc tcgtcggaga gggcgtgggc ctccccgacg ggcaggtcgg ccggcaccgg
     1081 aagcgagaag gagacgaaga gcccgtcctc gccgcgacga accgcgagcc cttccggccg
     1141 gaacgacggt cccctctcga tccgcagccg ctcgagctcg gatcggaccg gggcgtcgtc
     1201 ggcgggcgtc gtgcgccgcg cggccgtcgt ctcccccacg ggctcgatgt ggctgacgac
     1261 gtgcgcgacc tcgggcgccg cgcggcggag gttctcctcg aactcggaga cgaccgcgtg
     1321 ggcctcccgg acggtcaggc gctcgtccac ctccaggtgc agctccatcg agacgcttcc
     1381 gtccgtcgcg tgcgtccgga tcgcgtgggc gccgagcccg tgcctcgcgg cgaggaggcg
     1441 tgtcgtggcc aggagcccct cgctcccttc ggagaccggt tcgacgtgcg cgacgacgtc
     1501 gacgccgggg aggagcccct tcagcgccgc ctccgcggcg tcggaaatct cgtgcgcgcg
     1561 ctccagcgcg aggtggcggc cgacggtcag agtcgcgtcg acgaaggcgt ggggccccgt
     1621 ccggcggacg cgaagcgccg ggacgtcgac gacgcccggg acgcgcgccg cggccacgag
     1681 ccgctcccgg aggccgaccg ggaccgcgtc gacgaggtcg tcgatcgact tcttcccgag
     1741 gttcagcgag agggcgacga cgatcgccgc caccacgagc gcggcgacgg cgtcggcctt
     1801 cgcgagccag gcgacgcccg tccgctggga gacgacgacg agggcgagcc ccaggaggac
     1861 gacggccgag gaccagacgt cggtcgagaa gtggagcgcg tccgcctcga gcgcccggct
     1921 cccgtacttc ttcgcggtgc gcgcgagcgc ccgcgagcgc gagacgtcga cgacgatcga
     1981 gatcgccatc acggcgaagg cccaggcggt cggctcgacg tgcgccgggt ggaagaagag
     2041 acgctcgatc gcctcgtaga cgatccagag gcaggtcagg aggagcagcg cggtctcgaa
     2101 gagcgccgag aggttctcga tcttcccgtg cccgtaggtg tgctccgggt cggcggggcg
     2161 ccccgcggcc cggacggccc agagcgtcac gaccgcggcg acgaggtcga gcgacgagtg
     2221 ggccgcctcg gcgaggatgc cgatcgagcc tgtcgagacg ccgacggcga ccttcattcc
     2281 cgtcaggaag acggccgcca ggacggagga gagcgcgacg aagcgcttct cccggtcggc
     2341 cgtctcgaag ccttcccgaa tcgccgcgac cgtcacgggg cggcggcggc cctttccgcc
     2401 tctccctcgg cccggcgggc gagtcccgag acggcgccct ggaagaggcg gctccagcgc
     2461 gactggacga gaggcgcgaa gaggccgacc ggcctgccgt ggaggacctc gacgttcacg
     2521 acgaacgtcc gcgtcggcgt gaccggcgtg aagcgccaga cgtgacagga gacgatcccg
     2581 gcctcgtcgc ggcaccacgt cacctcgcgg ccgggcgtca ggcgcgtgac gaactcccgg
     2641 gcccgcacgg tcccgagcgg gaagccgagc gcgaggacct gctcgaagcc gccggcgacc
     2701 cggaagcccg gctcgccggt ccagcgcgcc ccgaggtgga ggggcgccga ccagagcggc
     2761 cactcctcga gacgggagaa gacctcccag gccttcgccg cgggagcgcc gacgcttccc
     2821 gaggaggcga cgacgagcgt cttccacgaa tcgagcggga cgccggccgc ttccgcggcg
     2881 gcgcgcgccg ccgccgtctc ggcctcggtc cccgagaacc cgacccggag cgcggacatc
     2941 gcgaagacgg ccgccacgag gaggaggagg aggaccgcgg agagcccgcc gagacgacgg
     3001 agggaacgcg acatccgggc gagtttaggc tcctggcgac ggaggggggc ccggcgacgg
     3061 aggcgcctct cgcgccgcgt cggcgccgga gggctttccg ctccccgcca ggaggaccgc
     3121 ggcgacgatc gccgctccgc cggcgagcgt cgcgagggag agcggctcgg cgaacgcgat
     3181 ccagccgagg agcgcgccga agacgacggt gagcgggttc gcgagcgtcg ccctcccggc
     3241 gggaactctc gcgaggccgc gcgtgaggaa gagctggccg agctggccgg ccgcggcgat
     3301 gaagacgagc cacccccact ccgccggcgt cggaatcgcg ggcccctcga cgagcgttcc
     3361 gaccgtcgcg agcgggatcg acgtcagggg aaaccagagg acgatcgtct cctcgtgctc
     3421 ggtgcgggac gcgtcgcgga cggtgacgta ggccgccgcg gagaggaggg cgccgagtag
     3481 gccgacgacg aggggcgcga cggacgagga cgcggccccc gcctccgccg ctcccctcgg
     3541 cacgaggagc gcgacgccgg acgccgcgag gaggaacgcc ggcacgaagc gcttccccgg
     3601 cttctccgac aggaaccacg cggcgaggac cgccgtgaag acggggtgga cctgctggag
     3661 gaggaccgcc gtcgcgagcg gctgccggga aacggaccag aagtagcagg agagggcgaa
     3721 gtgcccgagg gcgccgcgca ggacgaggag gcccggccgc ttcggcttcc aggagagccc
     3781 gcgcctccgg aggagccaga gcatgaagag cgcgttcagg acgcccctca cgaggatcac
     3841 ctcctgggga gggacccgcc ctccgaggag cttcgccccc gccgccatga cggcgaagaa
     3901 gaaggcggag agagccatct cgcgcacggc cggcgattat cggcgagggg ccggacggaa
     3961 cgcgccggtg cgatactccg cctcccttgg cccacctcgt cctcgccctg acggtcctcg
     4021 tctggtcgtt ctcgttcctg gcggcggcgc gcctgcgcca ggagctcggc ctcggggagg
     4081 ccctcgcggc gcgcttcgtc ccggtgctcc tcggcgccgg cgcgtacctc gccacgcgcc
     4141 gaccgctccg gatcccgcgc gcggcgtggc ccacggtcgt cgcgatgggc ctcctctcgg
     4201 tccccgtcta caacgtcttc ttcttccacg ggatgaagac ggtcccgagc ggaacggcgg
     4261 cgctcatcat cgcgctcaac cccgtcttca ccgccgtcct cgcgcgcgtc gtcctcggcg
     4321 agccgttcgg ccggcggcac gtcgccggcc tcgccctcgc gctcgccggc gtcttcgtcg
     4381 tgatccgcta cggcacggac aagagcgtcg actggcccta cctgtcgagc gccctcctcc
     4441 tcgcgctcgc ccccctctcc tgggcgttct acgcggtcct cggacggagg ctccccgccg
     4501 aggcggaccc gatcgacacg tcgtacgcgc tcctcttcgc cgggagcctc ccgctcctgg
     4561 cgttcgcgcg cccctccacg gccgcgaagc tggcctccag cccgggggcg ctcggcgccg
     4621 cgctctacct cgccgtcccc tgcacgctcc tcgcgtgggt ctcgttcctc tgggcgttga
     4681 agaggctccc cgcgggcgag gcggccgcct tcatcttcct gaacccgccg ctcgcgaacc
     4741 tctgggcatt cgtcctcgag gggcagccgc tccgaccgcc gttcctcgcg ggagcggcga
     4801 tcctcctcgc cggcgtcggg ctgatcgtcc tccggcccgg cgggaggagt aggcttccgg
     4861 cgtgagcggc ccttcgcacg ctcccggtgg cggcgcccag cgctacgcgc tcctctccat
     4921 cgccgcggcg ctcgccacga tcagcctgaa gacggtcgcg tggaaggtca ccggctcggt
     4981 gggccttctc tcggacgccg tcgagtccgt cgtcaacctc ctcgccgccg tcgtcgcgct
     5041 ctgggccctt cgtttcgccg cggcgccccc cgacgcggag catcagttcg gacacgcgaa
     5101 ggcggagtac ctcgcttcgg cgttcgaggg cctcgcgatc ctcggcgctt ccgtcgcgat
     5161 cgcgctcacg gcctgggagc ggatcgccga cccgcgcccc atcgagcagg cctggctggg
     5221 catcggcgtc tcgacggtgg ccgccgcggt caacggcggc gtcgccttcg tcctcttccg
     5281 cgccggccgg cggctcgact cgatcgcgct ccgatccgac tcccaccacc tgatgaccga
     5341 cgtctggacg tcggccggag tcctcgcggg cgtcgccctc gtcgagctga cgggctggca
     5401 tctcctcgac ccgatcgtcg ccctcggcgt ggcgacgaac atcgtctgga tggcggggag
     5461 aatcctcctc gagacggccc agggactcct cgaccgaacc attcccgaga ccgagaaggc
     5521 cttgctcgag gccctcctcg cacgctaccg ggacaacggc gtcgagctcc acgccgtgag
     5581 gacgaggcag tcgggtccgg tccgtttcgt cgacatgcac gtcctcgtcc ccgggcaatg
     5641 gaccgtccag cgcggccacg acctctgcga ggagatcgaa cgggaggtgc gggctcttct
     5701 gccgcgcgcc tcggtcctca cgcacctcga gcccgtcgac gacccggtcg cctggcacga
     5761 gccgggctcg ccgcccgctc cgcgctgaga acggcggaac agccgcacgg cgcggccggg
     5821 acgacgcgga ggatggaaaa gggtacgggc ggcgcccggg gagggcgccg ccgcgtcggg
//
LOCUS       DAOTEZ010000271         5841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_9804_length_5841_cov_4.141721, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000271 DAOTEZ010000000
VERSION     DAOTEZ010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9804_length_5841_cov_4.141721"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1351
                     /gene="accC"
                     /locus_tag="P5164_13580"
     CDS             <1..1351
                     /gene="accC"
                     /locus_tag="P5164_13580"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRY44969.1"
                     /translation="ANRGEIALRIIAACKELGIATVAVHSTVDRDALHVEAADESVCI
                     GPAAPSASYLNITSLVAAAEITGADAVHPGYGFLAENAHFAEILEEVGLTWVGPSPEA
                     IRRMGDKSTAKDTAKKAGVPTIPGSPGPVGSTEEALKLAERIGYPVLLKAVAGGGGRG
                     MRVARSGKELAAAFELATVEAERAFSNGSLYLEKYLAEPRHVEVQVFGDRLGKIVHLG
                     ERECSLQRRHQKVLEESPSPAVSPELREKMGAAAIAAARAVDYVGAGTVEFLLDQDGS
                     FYFMEMNTRIQVEHPVTEMVTGFDLVKEQIRVAAGEPLSFPRVGTAKDDFRPRGHAIE
                     FRINAEDPEKFTPSPGTITTLHVPGGPGVRVDTAAYIGWRIPPHYDSLLAKLIVHGQT
                     REEALARGRRALELFVVEGVKTTIPLHLRLLDAPDVRHGRFSTKWLERWLAGETPAA"
     gene            1365..1889
                     /locus_tag="P5164_13585"
     CDS             1365..1889
                     /locus_tag="P5164_13585"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRY44970.1"
                     /translation="MTAGPGRIFLVGFMGAGKSTVGRALAGRLEVPFLDLDEAFEALA
                     GVTIRRAFEERGEAWFREREAELLRGCEALPDVVVALGGGTFTFPENRAVVRRAGRSL
                     FLDVPFEAIEARLGEKAVDRPLFRSLEEARALYEERRASYRLSDTILALEGDEGVDAV
                     VDRIVGALESRGVG"
     gene            1894..2652
                     /locus_tag="P5164_13590"
     CDS             1894..2652
                     /locus_tag="P5164_13590"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRY44971.1"
                     /translation="MRYLLLSDLHSNDEALSAVLARVRRKKLDRVVCLGDFVGYAADP
                     NKVLDKIRTLPRPAVSIRGNHDKVVSGVDTGEMFNAPALLAARWTAEKLSKENLEFLR
                     RLPCGPVLVDESFAICHGSPLDEDAYIFSDWDAQMNFVHMDRVAPSVSVSFFGHSHIP
                     SIFTLASDGILVEAVRGSRRKLFLEPGRRYLVNPGSVGQPRDRNPRASYAIFDSEERT
                     VTFDRVPYDVKTARQKVLRAGLPTILGDRLLLGL"
     gene            2660..4726
                     /locus_tag="P5164_13595"
     CDS             2660..4726
                     /locus_tag="P5164_13595"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRY44972.1"
                     /translation="MEPRRLGTAKAGRAALVAVVLALGACSSLPAPSASEKARAARVE
                     AVAHLLEMEDRRLWDAPLAVAAAGSADAVLRERAAFAAGRIRPDDAPAFLTRLLSDRE
                     PRVRRAAAFAAGLAAERDLVPALRAAIADPDPETAAAAATALGRIGGAEAEDALREIL
                     RTASGPVAAAAAALQRSKDPALVPLLEGAAFSTDRESRRMAAWSLARAPRPGSASALR
                     RLLRDGDGEIVAWAARGLGLLGDADAAAALSEVALGREPGPAIQALLALERLAADGAV
                     RELARGAGLARRDDPHPGIAVAALTLLRRAADAADVRELLARAAASGGRRGGVALASL
                     ATGDAELAFGLAFPSSGAAPLDLRLGAAEALPLLAAERAGPWLEALLGDSAPRVRMEA
                     ISRFPRPLVPRSLPLLTRALADPDGAVRAAALDAAAPFAAGTGSDPTLAAAWRAAFDA
                     LVGSGEADLVVSALDAAASLPEGGRELLAAQRDAGDDLVRERARRLLRERFGEAPGAS
                     PRIVATRLAAADYRRLAERAEGPPVRVVVETTRGAFEAELFPYAAPMTVESFVSLARA
                     GFFDGTTIHRVVPDFVVQAGDPRGDGTGGPGWAIRDELNPVPYVRGRLGMALSGPDTG
                     GSQWFVALSRQPHLDGGYTVFGEVTAGMEVVDRIEQNDRLLSVRVRGEPASGGDAAGG
                     PVRGVN"
     gene            4735..>5841
                     /locus_tag="P5164_13600"
     CDS             4735..>5841
                     /locus_tag="P5164_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44973.1"
                     /translation="MPRFTVLETSALFSNRVKYDYLFEMEMWLKGVERFFVLDHLPLS
                     AFERSHASLKNFVEEVAVVRSGISHLALLTTHLLGEGKEDLASFLLFLDSQVSRASGG
                     ASVPQRRGSAETELALVVEKLDDFSKVLDELSRAPFLPLQTYLSLGRIVVGTLKSDPN
                     LGVLFRENLRPVLDRESKQSLARVLAAVSDASQKSALAALFVELFKGLRWTERIRQTI
                     ESPATIKRALLVFSLIRAQATSLGEFIRKRLLPRFPEDTPVAAGLERLVFSVEMEMRK
                     VMELELVDVALMKDPKLVYARMEDASGILKDLFQQSVIGLAETFSSDVDGRALFPEYR
                     TKLDQSLRLRDDLWELLQAVQAFSRGADKKALKAM"
BASE COUNT          741 a         2150 c         2096 g          854 t
ORIGIN      
        1 cgcgaaccgc ggggagatcg ccctgcggat catcgccgcg tgcaaggagc tcgggatcgc
       61 gaccgtcgcc gtccactcga cggtcgaccg ggacgcgctg cacgtcgagg ccgccgacga
      121 atcggtctgt atcgggccgg cggccccctc ggcgtcgtac ctcaacatca cctcgctcgt
      181 cgctgcggcc gagatcacgg gcgcggacgc ggtccacccg ggctacggct tcctcgcgga
      241 gaacgcccac ttcgccgaga tcctggagga ggtcggactc acctgggtcg gcccgtctcc
      301 cgaggcgatc cggcggatgg gggacaagtc gacggccaag gacaccgcca agaaggcggg
      361 ggtcccgacg atcccgggaa gcccggggcc cgtcggctcg accgaggagg cgctgaagct
      421 ggccgagcgg atcgggtacc cggtcctgct gaaggccgtc gcgggcggcg gcgggcgcgg
      481 gatgcgggtc gcgcggagcg ggaaggagct cgcggccgcg ttcgagctcg cgaccgtgga
      541 ggccgagaga gcgttctcga acggctccct ctacctcgag aagtacctgg ccgagccgag
      601 gcacgtggag gtccaggtct ttggcgaccg tctcgggaag atcgtccacc tcggcgagcg
      661 ggagtgctcg ctgcagcggc ggcaccagaa ggtgctcgaa gagtcgccct ctcccgcggt
      721 gtcgccggag ctccgggaga agatgggtgc ggccgcgatc gcggcggccc gagccgtcga
      781 ctacgtcggg gccgggacgg tggagttcct tctcgaccag gacggctcgt tctacttcat
      841 ggagatgaac acgcggatcc aggtcgagca cccggtgacc gagatggtga ccggcttcga
      901 cctggtgaag gagcagatcc gggtcgctgc cggcgagccg ctctcctttc cgagggtcgg
      961 caccgcgaaa gacgacttcc gcccgcgcgg ccacgcgatc gagttccgga tcaacgccga
     1021 ggacccggag aagttcacgc cttcccccgg gacgatcacg acccttcacg ttcccggggg
     1081 gccgggcgtg cgcgtcgaca ccgccgccta catcgggtgg cggatacccc cgcactacga
     1141 ctccctcctc gcgaagctga tcgtccacgg gcagacgcgc gaggaggcgc tcgcccgcgg
     1201 gcggcgagcg ctcgagctct tcgtcgtcga gggggtgaag acgacgatcc cgctccacct
     1261 ccggctcctc gacgcccccg atgtccggca cgggcgcttc tcgacgaaat ggctcgaacg
     1321 gtggctcgcc ggagagacgc cggcggcgtg accgccctcc cgtcgtgacg gccggacccg
     1381 ggcgcatctt cctcgtcggc ttcatggggg ccgggaagtc gacggtgggg cgcgcgctcg
     1441 ccggccgcct cgaagtccct ttcctcgatc tcgacgaagc gttcgaggcg ctcgcgggcg
     1501 tcacgatccg ccgcgcgttc gaggagcgag gcgaagcctg gttccgggag cgtgaggcgg
     1561 agctcctccg gggctgcgag gcgctcccgg acgtcgtcgt cgccctcggg ggcggcacct
     1621 tcacctttcc ggagaaccgg gccgtggtcc gccgggccgg acgatcgctc ttcctggacg
     1681 ttcccttcga ggcgatcgag gcgcggctcg gagagaaggc tgtcgaccgg cccctcttcc
     1741 gctcgctcga ggaggcgcgc gctctctatg aggagcggcg cgcttcctat agactttccg
     1801 acacgatcct ggcgctggag ggggacgagg gggtggacgc cgtggtcgat cgaatcgtcg
     1861 gggcgctcga gagccgggga gtcggctgag gaggtgcgct acctccttct ctccgacctg
     1921 cactcgaacg acgaggccct ctccgccgtc ctcgcgcgcg tgaggcgcaa gaagctcgac
     1981 cgcgtcgtct gcctcggcga tttcgtcgga tacgcggccg acccgaacaa ggtcctcgac
     2041 aagatccgca ccctgccccg cccggccgtc tcgatccgcg gcaaccacga caaggtcgtc
     2101 tccggcgtcg acaccggcga gatgttcaac gcgcccgcgc tcctcgcggc ccgctggacg
     2161 gcggagaagc tttcgaagga gaacctcgag ttcctgcgcc gcctgccgtg cggcccggtc
     2221 ctcgtggacg agtcgtttgc gatctgccac ggctcgccgc tcgacgagga cgcctacatc
     2281 ttctcggact gggacgccca gatgaacttc gtccacatgg accgtgtcgc gccgtccgtc
     2341 agcgtctcgt tcttcggcca cagccacatc ccctcgatct tcaccctcgc ctccgacggg
     2401 atcctcgtcg aggcggtccg ggggagccgg agaaagctct tcctcgagcc ggggcggcgg
     2461 tacctcgtga accccgggtc ggtcggccag ccgcgcgacc gcaacccgcg cgcgagctat
     2521 gcgatcttcg actcggagga gcggaccgtc acgttcgaca gggtgccgta cgacgtgaag
     2581 acggcccggc agaaggtcct ccgggccggc cttccgacga tcctcggcga ccggctcctc
     2641 ctggggctct gaggcgcgcg tggagcctcg gcgtctcggc acggcgaagg ccgggcgcgc
     2701 ggcgctcgtc gccgtagtcc tcgcgctcgg agcctgctcc tccctcccgg cgccgtccgc
     2761 cagcgagaaa gcgagggccg cgcgggtcga agccgtcgcg cacctcctcg agatggagga
     2821 ccggcgcctc tgggacgccc cgctcgcggt cgccgcggcc gggtcggccg acgccgtcct
     2881 ccgcgagcgg gcggccttcg cggccggccg aatccggccc gacgatgcgc ccgcgttcct
     2941 gacgcggctc ctgtcggacc gcgagccccg ggtccgccgg gcggcggcgt tcgccgccgg
     3001 cctcgccgcg gagagggacc tcgtgccggc gttgcgggcc gcgatcgccg accccgatcc
     3061 ggagacggcc gcggcagcgg cgacggcgct cggacggatc ggcggcgccg aggcggagga
     3121 tgccctccgc gagatcctgc gcacggcctc cgggccggtc gcggcggcgg ccgcggccct
     3181 tcagcgttcg aaggacccgg cgctggtgcc tctcctcgaa ggggccgcgt tctcgaccga
     3241 ccgggagtcg cggcgaatgg ccgcctggtc tctcgctcgc gctccccggc ccgggagcgc
     3301 atcggccctt cgccggcttc tccgggacgg ggatggggag atcgtggcct gggccgcgcg
     3361 ggggctcgga cttctgggcg acgccgacgc cgcagccgcg ctctcggagg ttgcgctcgg
     3421 gcgggagccc ggccccgcga tccaggcgct cctcgccctc gagcgcctcg cggcagacgg
     3481 ggccgtgcgg gagctcgcgc ggggggcggg gctggccagg cgggacgatc ctcatcccgg
     3541 gatcgccgtc gcggcgctca cgctcctccg gcgcgcggca gatgccgccg acgttcgcga
     3601 gctcctcgcc cgcgcggcgg cgtcgggcgg gcgccgcggc ggggtcgcgc tcgcgagcct
     3661 cgcgacgggc gacgcggagc tcgcgttcgg cctcgcgttc ccttcgagcg gagccgcccc
     3721 tctcgacctc cggctcgggg ccgccgaggc gctcccgctc cttgccgccg agcgagccgg
     3781 gccttggctc gaggcgctcc tcggggactc tgcgccgcgc gtccgcatgg aggcgatctc
     3841 ccgtttcccg cggcctctcg ttccccgcag ccttccgctc ctgacgcgcg ccctcgccga
     3901 cccggacggc gccgtccgcg ccgccgccct cgacgcggcg gcccccttcg ccgcggggac
     3961 ggggagcgat ccgacgctcg ccgcggcctg gcgcgcggcg ttcgacgcgc tcgtcgggtc
     4021 gggggaggcg gacctcgtcg tctccgcgct cgacgccgcc gcgtcgctgc ccgaaggggg
     4081 gcgggagctc ctggccgctc aacgcgacgc cggcgacgac ctcgtacgcg agcgggcccg
     4141 gcggctcctc cgggagcgct tcggcgaggc tccgggcgcc tcgccgcgga tcgtcgcgac
     4201 gcgcctcgcc gccgccgact accgtcgcct cgcggaacga gccgaggggc cacccgtccg
     4261 ggtcgtcgtc gagacgaccc ggggcgcctt cgaggccgag ctcttcccgt acgcggcccc
     4321 gatgacggtc gagagcttcg tctcgctcgc ccgcgcgggc ttcttcgacg ggacgacgat
     4381 ccaccgcgtc gtgccggact tcgtcgtcca ggcgggagac ccgcggggcg acgggaccgg
     4441 cggccccggc tgggcgatcc gcgacgagct gaacccggtc ccgtacgttc gcggccggct
     4501 cggcatggcg ctctccggcc ccgacaccgg gggctcgcag tggttcgtcg cgctctcccg
     4561 ccagccgcac ctcgacggcg gctacacggt cttcggcgag gtgacggcgg ggatggaggt
     4621 cgtggaccgg atcgagcaga acgaccggct cctctccgtg cgcgtccgcg gggagccggc
     4681 gtcgggcggg gacgccgccg ggggtcccgt ccgcggcgta aactgattcc agccatgccg
     4741 cgcttcacgg ttctcgagac ctccgctctc ttctcgaacc gcgtcaagta cgactacctc
     4801 ttcgagatgg agatgtggct gaagggggtc gagaggttct tcgtcctcga ccacctcccg
     4861 ctctccgcgt tcgagcggtc gcacgcgtcg ctgaagaact tcgtcgagga ggtcgcggtc
     4921 gtgaggtccg gcatctcgca cctcgcgctc ctgacgacgc acctcctcgg ggaagggaag
     4981 gaggacctcg cctcgttcct cctcttcctc gacagccagg tctcccgcgc ctcggggggc
     5041 gcctccgtcc cgcagcggcg cgggagcgcc gagaccgagc tggccctcgt cgtcgagaag
     5101 ctcgacgact tctcgaaggt cctcgacgag ctctcccggg ccccgttcct ccccctgcag
     5161 acctacctct ccctggggag gatcgtcgtc ggcacgctca agtccgatcc gaacctgggg
     5221 gtcctcttcc gcgagaacct gcggcccgtc ctcgaccgcg agtcgaaaca gtcgctcgcg
     5281 cgggttctcg ccgccgtctc cgacgcgagc cagaagtcgg cgctcgcggc cctcttcgtc
     5341 gagctcttca aggggctgcg ctggaccgag aggatccggc agacgatcga gtcgccggcg
     5401 acgatcaagc gcgccctcct ggtcttcagc ctcatccgcg cacaggcgac gagcctcggc
     5461 gagttcatcc ggaagcggct cctgcctcgt ttccccgagg acaccccggt cgccgcgggc
     5521 ctcgagcgcc tcgtcttctc cgtggagatg gagatgcgga aggtgatgga gctcgagctg
     5581 gtcgacgtcg ccctgatgaa agacccgaag ctcgtctacg cgcggatgga ggacgcctcc
     5641 gggatcctga aggacctctt ccagcagagc gtcatcggcc tcgcggagac gttctcctcc
     5701 gacgtcgacg gacgggcgct cttcccggag taccggacga agctggacca gagcctccgt
     5761 ctgcgcgacg acctctggga gctgctccag gcggtccagg ccttctcccg gggcgccgac
     5821 aagaaggcgc tgaaggcgat g
//
LOCUS       DAOTEZ010000272         5825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_9832_length_5825_cov_5.350433, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000272 DAOTEZ010000000
VERSION     DAOTEZ010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5825
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9832_length_5825_cov_5.350433"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="P5164_13605"
     CDS             <1..771
                     /locus_tag="P5164_13605"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY44974.1"
                     /translation="PRRTISFRGGLGALAEGLAEQGVRAGVRIELDAEIAGIEGPFDG
                     EGNGGVWRVQIADGTVYPADTLVSTLDAPAFAEALGPRLPRSGARLAALPYSRLAIVL
                     QAFRTESPGAALPGLGLLVPRGEGLRSLGVLYLSSLFPSRVPAGVALTTSFFGGALDP
                     SAPDLCESDLLRLAEAEVRRLHPLLGPRLHGRTLKWPSALPRLPVGHHETLRLLDADL
                     DDLNAGRERPRLVVTGTWRDGLGLGNRIARAEELAPLL"
     gene            783..2558
                     /locus_tag="P5164_13610"
     CDS             783..2558
                     /locus_tag="P5164_13610"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRY44975.1"
                     /translation="MRRLLPLSLAVALVLGAAAVPARSDRAARERPLHAGHPEGRAKR
                     ARALVSEAARAGSGRAALSPEGAERGYDVLTYDLRFELDPAVVALEGSAAIRIAALRP
                     GVSEVRLDLDDAMTVRGVERDGAPVAGFTAAGDVLTIPLTPPLGAEERTTLVVRWGGT
                     PLAGGALTFWTAPASGPAISSLAEPFDARTFWPCVDDPADKAVVTLSATVPSGYVAVS
                     AGRGASKSEPDGRVTWRWKLPQPISTYLVSVAVARFEEIAAEYRAADGRTMPVVGWVV
                     PEHAAPNRARVASMVRHLEVLASLFGEYPYLDTKYGIVEGSFSGGMEHPTITLIGASL
                     LGNASRDLTDLLVHELAHQWWGDEVTMRTWDDIWLNEGFATYAEILYHERASGLPPGF
                     LLASRYDDGLYAGQLGPAVVAPPSDPFRFTGAVYDKGAWVLHMLRRRVGDGAFFAAVR
                     EYRKRHERGSATRGDLRAVFEEVSERDLKQHFDQWVETPYRPVLRATFRNVSGGAEVN
                     VRQVQAHAVVHPRPGPDDVPHYVFPLVLRVFTANGGSKTVTVEVMRADETFFVPLDGG
                     AAAAGVALDPDGDLLEIVEAVGPAL"
     gene            complement(2576..3721)
                     /locus_tag="P5164_13615"
     CDS             complement(2576..3721)
                     /locus_tag="P5164_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44976.1"
                     /translation="MKATPRAVLALFLATYASSLLAQAPKPAPTAPSASGGAVVGADP
                     QGPPGSRPAEIQDVRVLGASPKDPWQMERLGVAAVQKNELGKAREFFEESWKVGELPT
                     APYNLACIDAREGKVDAAFRQLDRAIAAGFDDEATLAKDTDLASLRGRPEFARIVQGA
                     RRNRLAGDEAVVKEGVFVAPGRPPKGILLLLHEVNSDPMSIAGPFVAEAKARDLFVAA
                     PRGPARSGRKRFGWGSPDRALEALKRTIAEAQTRAGGLPIPVAVVGAGRGGKLGLEIA
                     ARNPGIFSAVASIGGIFDPGPTGAAGVAGLRGVPVFLGVAKSAPPELLRAMQRGRENL
                     QKLGVKVRWGEWPGTGEGLPADATAAAKDVLDAIRSPRPGAAVPPAK"
     gene            complement(3767..5275)
                     /locus_tag="P5164_13620"
     CDS             complement(3767..5275)
                     /locus_tag="P5164_13620"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRY44977.1"
                     /translation="MSAEASLRIVSPATGELLADVPAAGRAEVAAAVDLARAAAPAWA
                     ATPLPERIAALKRWRRAVVDDGELLRTLVRESGKPRHEAEGVELLYFCELARFVAAAA
                     RRALAPERRGTLLFAVRGTKVHRVPVGVVGVIGPWNFPILNNAADALAPLAAGNTVVL
                     KPSEVTPLTSLRMAELWRRAGNPPDVFRVVAGRGDAGAALVDLVDGVMFTGAVATGRK
                     VAARCGERLVPCVTELGGKSPLVVLAGADLARAAQSAAWSAYFHSGQACIRTERILVE
                     ERVADAFEKLFLERVKSLRQEAPDPDAAREHDLGAVTFPRQIEVVTRQLEDAVSKGAR
                     VLTGGKRRSGLAGAFFEPTVLAGVTPDMEVFREETFGPLVPIVRVANAGEALRLANDS
                     HLGLSASVWGPPRAARRLAERIEAGSANVNGALVHYFDVSAPLGGAKASGVGVRHGVE
                     GIRAFTRTRAVSVPRPLLAPLDRLLLSRLAFPYDRRVLGLLHRAIRLLYRRG"
     gene            5351..>5825
                     /locus_tag="P5164_13625"
     CDS             5351..>5825
                     /locus_tag="P5164_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRY44978.1"
                     /translation="MSSSPPGTGDYLSDVFFTDLPLPHAVRAGIAACGFVRATPVQAS
                     TLPLLLAGKDVAAQAQTGTGKTAAYLVSILTRLVEAPAPASRKPGAPRALIVAPTREL
                     AVQIEHDALLLSQFVRPRIVTVFGGLDYARQRSLLQAGCDILIGTPGRLLDYEGQG"
BASE COUNT          714 a         2249 c         2097 g          765 t
ORIGIN      
        1 ccgcggagga cgatcagctt ccggggaggc cttggggcgc tcgccgaggg gctcgccgag
       61 cagggggtcc gcgcgggggt gcggatcgag ctggacgccg agatcgccgg catcgaggga
      121 ccgttcgacg gagaggggaa cggcggcgtc tggagggtcc agatcgccga cgggaccgtc
      181 tacccggccg acaccctcgt ctccacgctc gacgcgccgg ccttcgcgga ggcgctcggc
      241 ccgcgcctcc cgcgctcggg tgcgcgtctc gcggcgctcc cctactcgcg cctcgccatc
      301 gtcctccagg cgttccgcac cgagtccccc ggggcggccc ttcccggtct cggcctcctc
      361 gtcccccgcg gggaggggct gcgctcgctc ggcgtcctct acctctcctc cctctttcct
      421 tcgcgcgtcc cggccggcgt cgcgctgacg acgtcgttct tcggcggagc actcgacccg
      481 tcggctcccg acctctgcga gagcgatctt ctccggctcg cggaggccga ggtgcgaagg
      541 ctccatccgc tcctcgggcc gcggctccac gggcggacgc tgaagtggcc gtcggcgctc
      601 ccgcggctcc ccgtcggcca ccacgagacg ctccgcctgc tggacgcgga cctcgacgac
      661 ctcaacgccg gacgcgagcg gccgcgcctc gtcgtcaccg gaacctggcg cgacgggctc
      721 gggctcggca accggatcgc ccgggccgag gagctcgccc ctctgctctg agtgtgaaac
      781 ggatgcgtcg tctcctcccg ctctccctcg ccgtcgcgct cgtcctcggg gccgccgccg
      841 ttcccgcgcg gtccgaccgg gccgcgcggg aacgcccgct tcacgccggc cacccggagg
      901 gccgggcgaa gcgcgcccgc gcgctcgtct cggaggcggc ccgggccggg agcggccggg
      961 cggcgctctc tccggaggga gcggaacggg gctacgacgt cctcacgtac gacctgcgct
     1021 tcgagctcga ccccgccgtc gtcgcgctcg aggggagcgc ggcgattcgg atcgccgcgc
     1081 tccgccccgg cgtctccgag gtgaggctcg acctcgacga cgccatgacg gtccgcggcg
     1141 tcgagcgcga cggcgccccg gtcgccggct tcaccgcggc cggagacgtc ctcacgatcc
     1201 cgctgacccc gccgctcggc gccgaggagc ggacgacgct cgtcgtccgc tggggcggga
     1261 ccccgctcgc gggcggggcc ctgacgttct ggacggcccc cgcgagcggt ccggcgatct
     1321 cctccctcgc cgagccgttc gacgcgcgga cgttctggcc ctgcgtcgac gaccccgccg
     1381 acaaggcggt cgtcacgctc tccgcgacgg tcccttcggg ctacgtcgcg gtctcggccg
     1441 ggcgcggcgc ctcgaagagc gagccggacg ggcgggtgac gtggcgctgg aagcttcctc
     1501 agccgatctc cacctacctc gtctcggtgg cggtcgcccg attcgaggag atcgccgcgg
     1561 agtaccgggc ggccgacggg cggacgatgc cggtcgtcgg atgggtcgtc cccgagcacg
     1621 ccgccccgaa ccgggcccgc gtcgcctcga tggtccgcca cctggaggtc ctggcctccc
     1681 tgttcgggga gtacccgtac ctcgacacga agtacgggat cgtcgaaggg agcttctcgg
     1741 gcgggatgga gcacccgacc atcaccctca tcggcgcctc gctcctcggg aacgcctcgc
     1801 gcgacctgac ggacctcctc gtccacgagc tggcccacca gtggtggggg gacgaggtga
     1861 cgatgcggac ctgggacgac atctggctga acgaagggtt cgcgacgtac gcggagatcc
     1921 tctaccacga gcgggcctcg ggtctccccc ccggcttcct gctcgcctcc cggtacgacg
     1981 acgggctcta cgccggccag ctcggccccg ccgtcgtcgc ccccccctcg gacccgttcc
     2041 gcttcacggg agcggtctac gacaaggggg cgtgggtcct ccacatgctc cgccgccgcg
     2101 tcggcgacgg ggcgttcttc gcggccgtcc gcgagtaccg gaagcggcac gagaggggca
     2161 gcgcgacgcg aggcgacctg cgcgccgtct tcgaggaggt ctcggaacgg gacctgaagc
     2221 agcacttcga ccagtgggtg gagacgccgt accggcccgt cctccgggcg acgttccgga
     2281 acgtctccgg cggcgccgag gtgaacgtga ggcaggtgca ggcccacgcc gtcgtccacc
     2341 cccggccggg tcccgacgac gtgccgcact acgtcttccc gctcgtcctc cgggtcttta
     2401 ccgcgaacgg cggctcgaag acggtgaccg tcgaggtgat gcgcgccgac gagacgttct
     2461 tcgtcccgct cgacggcggg gcggccgccg cgggggtcgc gctcgacccc gacggcgacc
     2521 tcctcgagat cgtggaggcg gtcggcccgg cgctctgagc cgggcctgcg gcctgctact
     2581 tcgccggcgg gaccgcggcc ccggggcggg gcgagcggat ggcgtcgagg acgtccttcg
     2641 ccgcagccgt cgcgtccgcc gggaggcctt cgcccgtccc cggccactct ccccaccgca
     2701 ccttcacgcc gagcttctgc aggttctccc ggccccgctg catcgcccgt aggagctccg
     2761 gaggggcgct cttggcgacg ccgaggaaga ccggcacgcc tcgcaggccg gcgactccgg
     2821 cggcgcccgt ggggcccggg tcgaagatcc ctccgatcga cgccaccgca gagaagatgc
     2881 cggggttgcg cgcagcgatc tccagcccga gcttcccgcc gcggcccgcg ccgacgacgg
     2941 cgaccgggat cggcaggccg ccggcccgcg tctgcgcctc ggcgatcgtc cgcttcagcg
     3001 cctcgagcgc gcggtcgggc gagccccacc cgaaacgctt ccggcccgag cgcgccggcc
     3061 cgcgcggggc ggcgacgaag aggtcccggg ccttcgcctc cgcgacgaag gggcccgcga
     3121 tcgacatcgg gtcggagttg acctcgtgca ggaggaggag gattcccttg ggcggcctcc
     3181 cgggagcgac gaagacccct tccttcacca ccgcttcgtc gccggccagg cgattccgcc
     3241 tcgccccctg cacgatccgg gcgaactcgg ggcgcccccg aagcgacgcg aggtcggtgt
     3301 ccttcgcgag cgtcgcctcg tcgtcgaagc cggcggcgat cgcgcggtcg agctgccgga
     3361 acgccgcgtc gaccttcccc tcgcgcgcgt cgatgcaggc gaggttgtag ggggcggtcg
     3421 gaagctcgcc caccttccac gactcctcga agaactcgcg cgccttgccc agctcgttct
     3481 tctggacggc ggcgaccccg agacgttcca tctgccaggg gtccttcggc gaggccccga
     3541 gcacccggac gtcctggatc tcggccggcc gggagcccgg aggcccctgc ggatcggctc
     3601 cgacgacggc cccgccggac gcggaggggg ccgtcggcgc cggcttcggc gcctgcgcga
     3661 ggagggagga cgcgtatgtc gcgaggaaaa gcgccagcac ggcgcgagga gtcgccttca
     3721 tggcggcgga ttgtagacgc ggggagaccg ggcgccttcg ggtccctcag cccctccggt
     3781 acaggaggcg gatcgcccga tggaggagac cgaggacgcg gcggtcgtag gggaacgcca
     3841 ggcgggacag gaggagccgg tcgagcggcg cgagcagggg cctcgggacg ctcacggcgc
     3901 gcgtccgggt gaaggcgcga atcccctcga cgccgtgccg gacgccgacg ccgctcgcct
     3961 tcgccccgcc gagcggcgcg gagacgtcga agtagtggac gagagccccg ttcacgttcg
     4021 cgctccccgc ctcgatccgc tccgcgaggc gccgcgccgc gcgcggcgga ccccagacgc
     4081 tcgcgctcag gccgaggtgc gagtcgttcg cgaggcggag cgcctccccc gcgttcgcga
     4141 cgcggacgat cgggaccagc ggaccgaacg tctcctcccg gaaaacctcc atatccggcg
     4201 tcacgcccgc gaggaccgtc ggctcgaaga acgctcccgc gaggccggag cgccgctttc
     4261 cgccggtgag gacccgcgcc cccttcgaga cggcgtcctc gagctgccgc gtcaccacct
     4321 cgatctggcg cgggaacgtg acggccccca ggtcgtgctc gcgcgcggcg tcgggatccg
     4381 gcgcctcctg tcgcagcgac ttcacccttt cgaggaagag cttctcgaac gcgtccgcga
     4441 cgcgctcctc gacgaggatc cgctccgtcc ggatgcacgc ctgcccgctg tggaagtagg
     4501 ccgaccaggc ggccgactgc gccgcccggg cgaggtccgc tccggcgagg acgacgagcg
     4561 gcgacttccc cccgagctcc gtgacgcacg ggacgagacg ttccccgcag cgcgccgcga
     4621 cctttcggcc ggtcgcgacg gcgcccgtga acatgacgcc gtcgacgagg tcgacgagcg
     4681 ccgccccggc gtcgcccctg cccgcgacga cgcggaagac gtcgggcggg ttccccgctc
     4741 tccgccagag ctccgccatc cggagcgagg tcagcggcgt cacctcggac ggcttcagga
     4801 cgaccgtgtt cccggcggcg aggggcgcga gggcgtcggc ggcgttgttc aggatcggga
     4861 agttccaggg cccgatcacc ccgacgacgc cgaccgggac gcgatggacc ttcgtcccgc
     4921 ggacggcgaa gaggagcgtg ccgcgccgct ccggcgcgag cgcccgccgg gccgcggcgg
     4981 cgacgaaccg ggcgagctcg cagaaataga ggagctccac cccttcggcc tcgtgccgcg
     5041 gcttcccgct ctcgcggacg agcgtccgga ggagctcgcc gtcgtcgacg acggcccgtc
     5101 gccagcgctt cagcgcggcg atccgctccg ggagcggcgt cgcggcccag gcgggggcgg
     5161 ccgcgcgcgc caggtcgacg gccgcggcga cctcggcccg gccggcggcg ggaacgtccg
     5221 cgaggagctc gcccgtcgcg ggcgacacga tccgcaggga ggcttcggcg ctcatcggcg
     5281 catgctactg ccgggaccgt ctcccggctc tccgaccccc cttcacgggg tccggataca
     5341 atccccccgg atgtcctctt ctccgccggg caccggcgac tacctcagcg acgtcttctt
     5401 caccgacctt cccctcccgc atgcggtccg ggccgggatc gccgcctgtg gcttcgtccg
     5461 ggcgacgccg gtccaggcga gcaccctgcc gctcctcctc gccggcaagg acgtggccgc
     5521 gcaggcccag acggggaccg gcaagacggc ggcatacctc gtctcgatcc tgacgcgcct
     5581 cgtggaggcg cccgccccgg cgagccggaa gccgggggcg ccgcgggccc tgatcgtcgc
     5641 gccgacgcgc gagctcgccg tccagatcga gcacgacgcg ctcctcctct cgcagttcgt
     5701 gaggccccgt atcgtgaccg tcttcggcgg cctcgactac gcgcggcaga ggagcctcct
     5761 ccaggcgggg tgcgacatcc tgatcggcac ccccggccgg ctcctcgact acgaggggca
     5821 ggggg
//
LOCUS       DAOTEZ010000273         5809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_9874_length_5809_cov_5.726973, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000273 DAOTEZ010000000
VERSION     DAOTEZ010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9874_length_5809_cov_5.726973"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="P5164_13630"
     CDS             <1..478
                     /locus_tag="P5164_13630"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HRY44979.1"
                     /translation="TNVDLFGLMFGNILTVDPRDLLALVAASTLVVLLLVLYFRPLLL
                     ASIDEDGAFAAGVNVARMRLLVLVLLALTVVVALKVVGILLVSALLVLPGAVARALFA
                     RWPGFLLGSVAAALVMVLGGLLLSVSLDVPSGAAIILTGTLLFLAAVGVGRLRRNA"
     gene            475..1179
                     /locus_tag="P5164_13635"
     CDS             475..1179
                     /locus_tag="P5164_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY44980.1"
                     /translation="MTAPLLSATALTRTYVAGEERVVGVRDAALSLERGELAVLAGPS
                     GSGKTTLLNLLGLLDRPDSGRLELLGEDVSTLDDRGRARLRREKLGFVFQSHQLVPVL
                     SAEENVALALWIRGLPDGECRSRARAALAAVGLAGLEGRRPDAMSGGQRQRVAVARAI
                     VGEPSLVLADEPTASLDSETAARLLDLFDALHAEKGIAFLFSSHDPRIVERVPRRVRL
                     VDGRIASDERTGGGAA"
     gene            1176..>2310
                     /locus_tag="P5164_13640"
     CDS             1176..>2310
                     /locus_tag="P5164_13640"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRY44981.1"
                     /translation="MTLRWWRLAARNLLRHRRRTFLTGSIVVVGYVAATMTAGFVAQT
                     FGGLKDITIRGQGGHLRVVSAEAEGKTDEEAASLLLDDADAVTRSVATSPDVKQAMPR
                     LTFFGLVAHGERSVGYMGTGGVPSLEKSATLAAEAVAAGTFLSDPEADEVMLGGGLAR
                     SLGVTVGDLVTVMATTPDGGLNAVDATVVAVLRWPIKELDDRILFLPYAPAARLLKAE
                     GKATSIAVTLKEGADLERSAGALRARLHRNGTPASVRTWIESAPFYVAVKRLYAAIFL
                     FTGLVLATVVVLAAANTMTMSVFERTREIGTLLAIGMERGQVRRLFLAEGLLMGLAGS
                     AAGAVASLLIRAAINAARIVLPPPPGATSGGALHVELIPLAFGV"
     assembly_gap    2311..2410
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2556..3311
                     /locus_tag="P5164_13645"
     CDS             2556..3311
                     /locus_tag="P5164_13645"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HRY44982.1"
                     /translation="MSDRRLAAGLVLLVFALLLLALAPSAPSAPPDAAAILARADVPR
                     TALSEARLRLRVTTAVAGAAAFTGEFTVLVKGPEKIRIEFLAPEDRGKLLLVNGKDAW
                     LVLPNTRNPIRVPRSHRVTGGFAVADVARTRFVDDYDATVERSDVLAGRTCDVLLLRA
                     KKGRDPAFPVLRVWVDRAESLTRKVVFLLPSGKTAREATFDEWGTLRGVRTVTRMTIV
                     DALRSGTTVVDYLDAEKATLDDALFLPQLPLRR"
     gene            complement(3325..4188)
                     /locus_tag="P5164_13650"
     CDS             complement(3325..4188)
                     /locus_tag="P5164_13650"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRY44983.1"
                     /translation="MLGGPPHPTLRDLWTEEDEEDVRAFLSAVTEDDLLRALSAVEGT
                     PAPVRIEDAAQLEAWAAVAARRLTVLPTTGPFALSRLLRGLLVDELGLKGDADDYSTT
                     RNCCLHQVLARRKGMPILLSCVWMVVGRRAGIPVEGIGMPGHFLVRVGGAAGVHVDTF
                     AGGQELSREGCRSLHDRIAGTKSRWSDDFLRPSPIDQVLERVLNNLAATTREENDAAG
                     SYRVATFLAALRPLSPERTLQKAMTAAAVGVTEEAASVFADVIERFPDSPEAERASQG
                     LDSLASGTTVN"
     gene            complement(4222..5166)
                     /locus_tag="P5164_13655"
     CDS             complement(4222..5166)
                     /locus_tag="P5164_13655"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRY44984.1"
                     /translation="MKPSTPRLRGAAALLALAALLGASCSPAPSPAPPPAPTPVPTPV
                     PTREVPAKVGLALGGGGARGFAEIGVLRVLEQEKIPISFVAGTSVGSLIGALYADTGR
                     VLDAEFHAVTVTEEDLFDYRALAVFSGGFVKGERLRAFLGANLKSRSIEQMKVPFAAV
                     AVDVASGRAVVFRRGPVAPAVHASCAIPGVFVPVEIGGRTYVDGGVANPVPADVVRSM
                     GADVVVAVAIPALTPATIPKNPVAVAYQSVMIMSAEIGRLRAREADVVVTPDVGDVAY
                     DDFGQKKRLITAGEEATRRALPEIRAAIAAKTRRVPVS"
     gene            complement(5163..>5809)
                     /locus_tag="P5164_13660"
     CDS             complement(5163..>5809)
                     /locus_tag="P5164_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY44985.1"
                     /translation="HLLSVQPGRWEAALASPWAAFADRAFLPRFLHFVLAGVTLAAIL
                     LAWLAVRRPPAEEERAAAGARASWGVKLALLATLLQLVVGLWLTISVPRDVLLGLMRG
                     GGRTMAPFTLGIAAGFGLLVFLARVRDPFADRGSVRRALELYGLAMLLMLVTRHQLRD
                     VYVDAAGAAAAPAAASQWGVFAIFLAAFVACAALAILALRRAATDRPGPGEATA"
BASE COUNT          728 a         2078 c         2128 g          775 t
ORIGIN      
        1 gacgaacgtc gacctcttcg gcctcatgtt cgggaacatc ctgacggtcg acccgcggga
       61 cctcctcgcc ctcgtggccg cctcgacgct cgtcgtcctc ctcctcgtcc tctacttccg
      121 gccgctgctg ctcgcctcga tcgacgagga cggcgcgttc gcggccggcg tgaacgtggc
      181 gcggatgagg ctcctcgtcc tcgtcctcct cgccctgacg gtcgtcgtcg cgctgaaggt
      241 cgtcgggatc ctcctcgtct ccgccctcct cgtccttccc ggcgccgtcg cccgggctct
      301 cttcgcgcgc tggcccggct tcctcctcgg gtcggtggcg gcggcgctcg tgatggtcct
      361 cggcgggctc ctcctttccg tctcgctcga cgtcccttcg ggggcggcga tcatcctcac
      421 gggcacgctc ctcttcctcg cggcggtcgg cgtcggtcgg ctccggcgga acgcgtgacg
      481 gcgccgcttc tctctgcgac cgccctgacc cggacctacg tggcgggcga ggagcgggtc
      541 gtcggcgtga gggacgccgc gctctccctc gagcggggcg agctcgccgt cctcgccgga
      601 ccctcgggaa gcgggaagac gacgctcctg aacctcctcg gcctcctcga ccggcccgac
      661 tccggccggc tcgagctcct cggcgaggac gtctcgacgc tcgacgaccg cggtcgcgcg
      721 cgcctgcggc gcgagaagct cggcttcgtc ttccagtcgc accagctcgt cccggtcctg
      781 tcggccgagg agaacgtcgc cctcgcgctc tggatccgcg ggctcccgga cggggagtgc
      841 cgctcccgcg cgcgggcggc gctcgccgcg gtcggcctcg caggcctgga ggggcgccgc
      901 cccgacgcga tgtcgggcgg gcagcgacag cgggtcgccg tcgcgcgagc gatcgtcggc
      961 gagccgtcgc tcgtcctcgc ggacgagccg accgcctcgc tcgactccga gacggccgcg
     1021 cgcctcctcg acctcttcga cgccctccat gccgagaagg ggatcgcgtt cctcttctcg
     1081 agccacgacc cgcggatcgt ggagcgggtc ccgcggcgcg tccgcctcgt ggacggccgg
     1141 atcgcctcgg acgagcggac cgggggaggg gcggcgtgac gctccgctgg tggcgcctgg
     1201 cggctcggaa cctcctccgg caccggcgcc ggacgttcct gacggggagc atcgtggtcg
     1261 tcggatacgt cgccgcgacg atgacggccg gcttcgtcgc ccagaccttc ggcgggttga
     1321 aggacattac gatccgaggc cagggcggac acctccgcgt cgtatcggcg gaggccgaag
     1381 ggaagaccga cgaggaggcg gccagcctcc tcctggacga cgccgacgcg gtgacgcgga
     1441 gcgtggcgac gagcccggac gtgaagcagg cgatgccgcg gctcacgttc ttcggcctcg
     1501 tcgcgcacgg agagcggagc gtcggctaca tggggacggg cggagtgccg tcgctcgaga
     1561 agagcgcgac gctcgcggcc gaggcggtcg ccgccgggac cttcctctcc gatccggagg
     1621 ccgacgaggt gatgctgggc ggcggcctcg cccgctccct cggcgtgacg gtcggcgacc
     1681 tcgtcacggt catggcgacg acccccgacg gaggcctgaa cgccgtcgac gccacggtcg
     1741 tggcggtcct ccgttggccg atcaaggagc tcgacgaccg gatcctcttc ctcccgtacg
     1801 ccccggcggc gcgcctcctg aaggccgaag ggaaagcgac ctcgatcgcc gtgacgctga
     1861 aggaaggggc cgatctcgag aggtcggccg gcgcgctccg ggcgcggctc caccggaacg
     1921 gcacgccggc ctccgtgagg acctggatcg agagcgcccc gttctacgtc gccgtgaagc
     1981 ggctctacgc ggcgatcttc ctcttcacgg gcctcgtcct ggccacggtc gtcgtcctcg
     2041 ccgcggcgaa cacgatgacg atgtcggtct tcgagaggac ccgggagatc gggactctcc
     2101 tggcgatcgg gatggagcgc ggccaggtga gaaggctctt ccttgccgag gggctcctga
     2161 tgggcctcgc cggctccgcg gccggggccg tcgcgtcgct cctcatccgc gcggcgatca
     2221 acgcggcgcg gatcgtgctc cccccgcctc cgggcgccac gagcggggga gcgctccacg
     2281 tggagctgat cccgctcgcc ttcggagtcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn gccacgagcg ggggagcgct ccacgtggag ctgatcccgc tcgccttcgg
     2461 agtcgggttc gtcctgatga cggccacgct cctcgtcgcg tcgtggtggc cggcgcggcg
     2521 ggccgcgcgc ctcgaccccg tggaggcgct cgcccatgtc tgaccggagg ctcgcggccg
     2581 gcctcgtcct gctggtcttc gcgctcctcc tgctggccct cgcgccctcc gcgccgagcg
     2641 ctccgccgga cgcggccgcg atcctggccc gggccgatgt cccgcgaacc gccctctccg
     2701 aggcgcgcct gcgccttcgc gtgacgacgg cggtcgccgg cgcggcggcg ttcaccggcg
     2761 agttcaccgt cctcgtgaag gggccggaga agatccggat cgagttcctc gcgccggagg
     2821 accgcggcaa gctcctcctc gtgaacggga aggacgcctg gctcgtcctg ccgaacaccc
     2881 gcaacccgat ccgcgtgccg cgcagccacc gcgtgaccgg cggcttcgcc gtcgcggacg
     2941 tcgcgcggac ccgtttcgtc gacgactacg acgcgaccgt cgagcggtcg gacgttctcg
     3001 cggggaggac ctgcgacgtc ctcctcctcc gggcgaagaa ggggcgcgat ccggcgtttc
     3061 cggtcctccg cgtctgggtc gaccgggccg agtcgctcac gaggaaggtc gtcttcctcc
     3121 ttccctccgg gaagacggcg cgcgaggcga cgttcgacga gtgggggacg ctccgcgggg
     3181 tccggacggt gacgcgaatg acgatcgtcg acgcgctccg gtccggcacg acggtcgtcg
     3241 actacctcga cgccgagaag gcgacgctcg acgacgccct cttcctcccg cagctcccgt
     3301 tgcgccgctg acgcggcccg ccggtcagtt caccgtagtc ccggaggcga gcgagtcgag
     3361 cccctgggag gcgcgttcgg cctcgggcga gtcggggaat cgctcgatga cgtcggcgaa
     3421 gacgctggcg gcctcctccg tgacgccgac ggcggcggcc gtcatcgcct tctggagcgt
     3481 ccgctcgggc gagagcggac ggagcgccgc gaggaacgtg gcgacgcgat agctccccgc
     3541 cgcgtcgttc tcctcccgtg tcgtcgccgc caggttgttc aggacccttt cgaggacctg
     3601 gtcgatcggg ctcggccgga ggaagtcgtc gctccagcgg ctcttcgtcc cggcgatccg
     3661 gtcgtgcagg gagcggcacc cttcgcgcga aagctcctgg ccacccgcga aggtgtcgac
     3721 gtggacgcct gccgccccgc ccacgcggac gaggaagtgg cctggcattc cgatcccctc
     3781 gacggggatt cccgcgcgcc gaccgacgac catccagacg cacgacagga ggatcggcat
     3841 ccccttgcgc ctggcgagaa cctggtgaag acagcagttg cgcgtcgtcg agtagtcgtc
     3901 ggcgtcgccc ttgaggccga gctcgtcgac gaggaggcct cgcaggaggc gtgagagagc
     3961 gaacggcccc gttgtcggaa ggaccgtcag ccgccgcgcg gccacggcgg cccaggcctc
     4021 gagctgggcc gcgtcctcga tccggaccgg cgcgggggtc ccttctacgg ccgagagggc
     4081 tcggaggagg tcgtcctcgg tcacggccga caggaaggcg cggacgtcct cctcgtcctc
     4141 ctcggtccag aggtcgcgga gcgtcgggtg gggcgggcct ccgagcacgc cctgatccta
     4201 gcgcgagccg gcccgggggc ctcaggaaac ggggacgcgg cgcgtcttcg ccgcgatcgc
     4261 cgcgcggatc tcgggaaggg cccttcgggt cgcctcctcg cccgccgtga tgaggcgctt
     4321 cttctgtccg aagtcgtcgt aggccacgtc gcccacgtcg ggcgtgacga cgacgtccgc
     4381 ctcgcgcgcg cgcaggcgcc cgatctcggc cgacatgatc atgaccgact ggtaggcgac
     4441 ggcgaccggg ttcttcggaa tcgtcgcggg cgtgagggcc ggaatcgcga ccgcgacgac
     4501 gacgtcggcc cccatcgagc ggacgacgtc cgccgggacg gggttcgcga cgccgccgtc
     4561 gacgtaggtc cgtccgccga tctccacggg gacgaagacg cccgggatcg cgcaggaggc
     4621 gtggacggcc ggcgcgaccg ggccgcggcg aaagacgacc gccctgccgg aggcgacgtc
     4681 gacggcgacg gcggcgaagg ggaccttcat ctgctcgatc gaccggctct tcaggttcgc
     4741 gccgaggaac gcgcgaaggc gctcgccctt cacgaagccg ccggagaaga cggcgagggc
     4801 gcggtagtcg aagaggtcct cctcggtcac cgtcacggcg tggaactcgg cgtcgaggac
     4861 gcgccccgtg tcggcgtaga gcgcgccgat caggcttccg acggaggtcc ccgcgacgaa
     4921 cgagatcggg atcttctcct gctcgaggac gcgcaggacg ccgatctcgg cgaagccgcg
     4981 cgcgccgccg ccgccgagcg ccaggccgac cttcgccggc acctcgcgcg tcggcacggg
     5041 ggtcgggacg ggcgtcggcg ccggtggggg cgcgggggac ggggccggcg agcacgaagc
     5101 gccgaggagc gccgcgagcg cgaggagggc cgccgctccc cggaggcgcg gagtcgaggg
     5161 cttcatgccg tcgcctctcc cgggccgggc cggtccgtcg cggcccgccg cagggcgagg
     5221 atcgcgaggg ccgcgcaggc gacgaacgcg gcgaggaaga tcgcgaagac gccccactgc
     5281 gaggcggcgg ccggagcggc ggccgccccg gccgcgtcga cgtagacgtc gcgcagctgg
     5341 tggcgcgtca cgagcatcag gagcatcgcg aggccgtaga gctcgagggc gcgccgcacg
     5401 gagccgcggt cggcgaacgg gtcgcggacc cgggcgagga agacgagaag gccgaagccg
     5461 gccgcgatgc cgagcgtgaa cggcgccatc gtcctgccgc cgccgcgcat caggccgagg
     5521 aggacgtccc gcgggaccga gatcgtcagc cagaggccga cgacgagctg gaggagcgtc
     5581 gcgaggaggg cgagcttcac cccccacgag gcgcgggcgc cggccgcggc gcgctcctcc
     5641 tccgcgggcg ggcgccggac ggcgagccag gcgaggagga tcgccgcgag cgtcacgccg
     5701 gcgaggacga agtggaggaa gcgggggagg aacgcccggt cggcgaacgc cgcccaggga
     5761 ctcgcgagcg ccgcctccca gcggccgggc tggaccgaga ggaggtgga
//
LOCUS       DAOTEZ010000274         5765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_9977_length_5765_cov_5.777233, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000274 DAOTEZ010000000
VERSION     DAOTEZ010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9977_length_5765_cov_5.777233"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..137
                     /gene="rpmE"
                     /locus_tag="P5164_13665"
     CDS             <1..137
                     /gene="rpmE"
                     /locus_tag="P5164_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRY44986.1"
                     /translation="ICSNCHPFFTGKAKLIDTAGRVERFQKRYAKSAAAVAAAATPSA
                     "
     gene            183..1265
                     /gene="prfA"
                     /locus_tag="P5164_13670"
     CDS             183..1265
                     /gene="prfA"
                     /locus_tag="P5164_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRY44987.1"
                     /translation="MLEKLDEIERRYASLEERLGSPDVASDHQESTKIHRAMREIAPV
                     VGKVREMRKAQADLAGALEMLATLPAGDELREMAQGEADDLKQSVAALEEEIRILLLP
                     KDPNDGKNVILEIRAGTGGDEAALFAEELFRMYVRYAEGRGWRVEVIDRSESGGRGGL
                     KEVQAIVEGEGAFSRLKYEGGVHRVQRVPETESQGRIHTSAATVAVLPEAEEVELEIA
                     EKDLRLDLFCASGPGGQGVNTTYSAVRITHLPTNTVVQCQDERSQIKNKAKAMRVLRA
                     RLFEVERERQEAAYAADRKKMVGSGDRSEKIRTYNFPQSRITDHRIGFTSHRLAEVLD
                     GRLDELIDPLVAHFQAEKLKMELAKA"
     gene            1372..2883
                     /gene="pap"
                     /locus_tag="P5164_13675"
     CDS             1372..2883
                     /gene="pap"
                     /locus_tag="P5164_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HRY44988.1"
                     /translation="MFETAELGRKLSKEEYAEALPAVRARLLAAQLALRTTKVPVIVV
                     IAGADGSGKGDTVNRLLEWLDPRGVETNVFGPRTDEERDRPAAWRFWRTLPARGRIGI
                     FFGSWYTEPVLKRTTGKMGGARFEAELARVAFFEESLAKDGALFVKCWFHLSKEAQKK
                     RLRKAEKDPEGRFKVTRLDWKHFGRYDRFTKVAERAIRATDTGEAPWTIVEAADDRWR
                     DLAVGRALAEALEARLASVKEADEKRAAASPPPRKTARATRPGRSAPARDGVLSTIDL
                     GRTVSDEDYRKELPRLQERLSKLACEGHRKGVSSVAVFEGSDAAGKGGAIRRVTWALD
                     ARLYRVIPIAAPTDEERAHHYLWRFWRHVPPGGSFTIYDRSWYGRVLVERVEGFAKEE
                     EWRRAYLEINDFETQLVESGIALSKLWLHISPEEQLRRFRERETVEWKKHKITDEDWR
                     NREKRDAYEGAIDEMVARTSTRDAPWTLVAATDKKAARLAVLSTLSDRLEEAL"
     gene            2988..3758
                     /locus_tag="P5164_13680"
     CDS             2988..3758
                     /locus_tag="P5164_13680"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRY44989.1"
                     /translation="MKTFPAFRPLRGGLVPLAFLIAACSPKAPEASKVPAGPPGKLGT
                     AAVAGRVILEGVPPANTRVTMTTDPFCNGRHPGETEMPEVAVGSEGALENVLLRVTEG
                     ASGVYPAPAEAKTLDQKGCAYSPRVLAIMTGQPLKIVSSDATIHNVHAAAKENRAFNL
                     GMPAPGARYTRTFEKPEIVPFRCDVHPWMRAFVAVVPHPFFAVTGADGRFEIRGLPAG
                     TYTVEAWHEKLPTQTFTLTLADGQAATRDVVFSAVPSA"
     gene            3755..4747
                     /locus_tag="P5164_13685"
     CDS             3755..4747
                     /locus_tag="P5164_13685"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HRY44990.1"
                     /translation="MSDDGEMTPSRPVRIAARTVAVSVFLLLIAGALVTSTGSGLAVP
                     DWPLSYGKLMPPMVGGILYEHGHRMIAATVATMVGLQILVLGFRRTDRTTFRLGLAAF
                     GAILAQAVLGGLTVLFLLPPAISSAHAGLAQIVFALTATIALRTSLFWARFTDAAPRA
                     AVEHGLRARASRLALWAAGATYVQILLGAIVRHTGAGLAIPDWPLSFGKVVPTGAEWT
                     GDGVLAHFTHRTFAWAVAALVVAAALALRKLDALPGLRRLSTLWLVLVALQVALGATS
                     VWTAKAVWATSAHLAVGGLLWIAGVLAAVLLARAAALSPDAAVAEAGEPQEAAA"
     gene            4744..5619
                     /gene="cyoE"
                     /locus_tag="P5164_13690"
     CDS             4744..5619
                     /gene="cyoE"
                     /locus_tag="P5164_13690"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HRY44991.1"
                     /translation="MKRVLADLLELTKPRITVFVVLTAFLGFAAGTDGPLAGIDPLLL
                     LHTLLGTALVASGTSAFNEIAEVDLDRRMARTATRPLPAGRLPFGGAVAFATLLSLLG
                     VAELWLFANGVTAFLAALTLTSYVFVYTPMKTRSPLSTIVGAVPGALPPLGGYTAAAG
                     AIGAPGLALFAILFLWQMPHFYAIGWRHREDYGAAGFRILSTVDPTGRSSARHALAFG
                     LLLVPVSVLPTALGTAGLVYAAGAFLLSAWFAGTAVTFFRETTDRAAKSMFLASIAWL
                     PAIVVLLLADRVSGG"
     gene            5616..>5765
                     /locus_tag="P5164_13695"
     CDS             5616..>5765
                     /locus_tag="P5164_13695"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY44992.1"
                     /translation="MSATPLVEVEGLSRSYGDRLALDAVSFGVEEGELFALLGPNGGG
                     KTTLFK"
BASE COUNT          802 a         2194 c         1947 g          822 t
ORIGIN      
        1 agatctgctc gaactgccac cccttcttca ccggcaaggc caagctgatc gacacggccg
       61 gccgtgtcga gcgcttccag aagcgctacg cgaagtcggc tgcggccgtc gccgcggccg
      121 cgacgccaag cgcctgagga ggccggtccc ctccctcccc ttctgacccg ggaaccgacg
      181 ggatgctcga gaagctcgac gagatcgaaa ggcgctacgc ctcgctcgag gagcggctcg
      241 gcagcccgga cgtcgcctcc gaccaccagg agtcgacgaa gatccatcgg gcgatgcggg
      301 agatcgctcc cgtcgtcggg aaggtgcgcg agatgcgaaa ggcgcaggcc gacctggccg
      361 gcgcgctcga gatgctcgcg accctcccgg ccggagacga gctgcgcgag atggcccagg
      421 gggaagccga cgacctgaag cagtccgtcg ccgcgctgga ggaggagatc cggatcctcc
      481 tcctcccgaa ggacccgaac gacggcaaga acgtgatcct cgagatccgg gccgggacgg
      541 gcggcgacga ggccgccctt ttcgccgagg agctcttccg gatgtacgtc cgctacgccg
      601 aggggcgcgg ctggcgtgtc gaggtgatcg atcgctccga gtcgggcggc cgcggcggcc
      661 tcaaggaggt ccaggcaatc gtcgaaggcg agggcgcctt ctcgcgcctc aagtacgaag
      721 ggggcgtcca ccgcgtccag cgggtccccg agacggaatc gcaggggcgc atccacacct
      781 cggccgcgac cgtggccgtc ctcccggagg ccgaggaggt cgagctcgag atcgccgaga
      841 aggaccttcg gctcgacctc ttctgcgcct cgggcccggg cggacagggg gtcaacacga
      901 cctactcggc cgtccggatc acccacctcc cgacgaacac cgtcgtccag tgccaggacg
      961 agcgctcgca gatcaagaac aaggcgaagg cgatgcgcgt cctccgggcg cgcctcttcg
     1021 aggtcgagcg ggagcggcag gaagcggcct acgccgccga ccggaagaag atggtcggct
     1081 cgggcgaccg cagcgagaag atccgcacct acaactttcc ccagtcccgg atcacagacc
     1141 accggatcgg gttcacgtcc caccgcctcg cggaggtcct cgacggccgg ctggacgagc
     1201 tgatcgaccc gctcgtcgcc catttccagg ccgagaagct caaaatggag ctcgccaagg
     1261 cctgacgtcc ccggggctcg tccgagcggc gcgatccgtt tcgccgcgcc gtccccgttt
     1321 cttcctcgcc ttcgcacgtc ccgcccgcgg ggccacggcc gggaggagac cgtgttcgag
     1381 acggctgagc tgggacggaa gctctcgaag gaggagtacg ccgaggcgct ccccgcggtc
     1441 cgcgcgcggc tcctcgcggc gcagctcgcg ctccggacga cgaaggtccc cgtgatcgtc
     1501 gtcatcgccg gggccgacgg ctccggcaag ggggacacgg tcaaccgcct cctcgagtgg
     1561 ctcgacccgc gcggcgtgga gacgaacgtc ttcgggccgc ggaccgacga ggagcgcgac
     1621 cgccccgcgg cgtggcgctt ctggcggacg ctcccggcgc ggggccgtat cggcatcttc
     1681 ttcggctcct ggtacacgga gccggtcctc aagcggacga ccgggaagat ggggggcgcc
     1741 cgcttcgagg ccgagctcgc gcgcgtcgcg ttcttcgagg agtcgctcgc gaaggacggc
     1801 gcgctcttcg tgaagtgctg gttccacctc tcgaaggagg cgcagaagaa gcgcctccgg
     1861 aaggccgaga aggaccccga aggacgcttc aaggtgacgc gcctcgactg gaagcacttc
     1921 ggccggtacg accgcttcac gaaggtcgcc gaacgggcga tccgcgcgac cgacacgggc
     1981 gaagcgccct ggacgatcgt cgaggccgcg gacgaccgct ggcgggacct cgccgtcggg
     2041 cgggccctcg ccgaggcgct cgaggcgcga ctcgcgtccg tgaaagaggc cgacgagaag
     2101 agggccgccg cctcgccgcc cccgcgcaag accgcgaggg caacgaggcc ggggcggagc
     2161 gccccggccc gggacggcgt cctctccacc atcgacctcg ggcggaccgt ctccgacgag
     2221 gattaccgga aggagctccc gcggctgcag gagcgactct cgaagctcgc ctgtgagggg
     2281 caccggaagg gggtctcgag cgtcgccgtc ttcgagggct ccgacgccgc gggaaaggga
     2341 ggcgccatcc gtcgcgtcac atgggcgctc gacgcccgcc tctaccgcgt catcccgatc
     2401 gccgcgccga cggacgagga gcgcgcgcac cactacctct ggcgcttctg gcggcacgtc
     2461 ccgccgggag gcagcttcac gatctacgac cgctcgtggt acgggcgcgt cctcgtcgag
     2521 cgggtcgagg gattcgcgaa ggaggaggag tggaggcggg cctacctcga gatcaacgac
     2581 ttcgagaccc agctcgtcga gtcggggatc gcgctctcga agctctggct ccacatctcc
     2641 cccgaggagc agcttcgccg cttccgcgag cgggagaccg tcgagtggaa gaagcacaag
     2701 atcaccgacg aggactggcg gaaccgcgag aagcgggacg cctacgaggg cgcgatcgac
     2761 gagatggtcg cgcgcacctc gacgcgggac gccccgtgga cgctcgtcgc cgcgaccgac
     2821 aagaaggccg cgcgactcgc cgtcctttcg acgctcagcg accggctcga ggaggcgctc
     2881 tgacgttccg ctcgcctccg gccgcagaga tttgacagag tctcagcccg gaaatagagt
     2941 cgatcgtttt gatgcggccg gacttccggt ccggcgcctc gaaaagcatg aagaccttcc
     3001 ccgccttccg tcccctccgg gggggactcg ttcccctcgc cttcctcatc gccgcctgca
     3061 gtccgaaagc gcccgaggcc tcgaaggtcc cggcgggtcc cccggggaag ctcgggacgg
     3121 cggccgtcgc aggccgcgtc atcctggaag gcgtcccccc ggcgaacacc cgggtgacga
     3181 tgacgaccga tccgttctgc aacgggcggc acccgggcga gaccgagatg cccgaggtcg
     3241 ccgtcggctc cgagggcgcg ctcgagaacg tcctcctccg ggtgacggag ggagcgagcg
     3301 gcgtctaccc ggctcccgcg gaggcgaaga cgctcgacca gaaggggtgc gcctactccc
     3361 cgcgcgtcct cgcgatcatg acgggccagc cgctcaagat cgtctcctcc gacgcgacga
     3421 tccacaacgt ccacgccgcc gcgaaggaga accgggcctt caacctcggc atgcccgcgc
     3481 ccggcgcccg ctacacgaga acgttcgaga agcccgagat cgtccccttc cgatgcgacg
     3541 tccacccctg gatgcgcgcc ttcgtcgccg tcgtcccgca ccccttcttc gcggtgacgg
     3601 gggccgacgg ccgcttcgag atccgcggcc tccccgcggg gacctacacc gtcgaggcgt
     3661 ggcacgagaa gctcccgacg cagaccttca ccctcaccct cgcggacggc caggcggcga
     3721 cgcgcgacgt cgtcttctcc gccgtcccct ccgcgtgagc gacgacggcg agatgactcc
     3781 ctcccggccc gtccgcatcg ccgcgcgcac cgtcgcggtc tccgtcttcc tcctgctgat
     3841 cgccggcgcg ctcgtcacct cgaccggctc cggcctcgcc gttcccgact ggcccctctc
     3901 ctacgggaag ctgatgccgc cgatggtcgg agggatcctc tacgagcacg gccaccggat
     3961 gatcgcggcg accgtcgcga cgatggtggg cctgcagatc ctcgtcctcg gcttccggcg
     4021 gaccgaccgg acgacgttcc ggctcggcct cgccgccttc ggcgcgatcc tcgcgcaggc
     4081 ggtcctcggc gggctgacgg tcctcttcct cctcccgccc gcgatctctt cggcgcacgc
     4141 gggcctcgcg cagatcgtct tcgccctcac ggcgacgatc gcgctccgca cctcgctctt
     4201 ctgggcacgc ttcacggatg cggcgccgcg ggccgccgtc gagcacggtc tccgcgcgcg
     4261 cgcctcccgc ctcgccctct gggccgcggg ggcgacgtac gtccagatcc tcctcggcgc
     4321 gatcgtgagg cacacgggcg ccggcctcgc gatccccgac tggcccctct ccttcggcaa
     4381 ggtcgtcccg acgggggccg agtggaccgg cgacggcgtc ctcgcccact tcacgcaccg
     4441 cactttcgcg tgggccgtcg ccgcgctcgt cgtcgcggcc gcgctggcgc tccggaagct
     4501 cgacgccctc cccggcctcc gccgcctgtc gacgctctgg ctcgtcctcg tcgcgctcca
     4561 ggtcgccctc ggcgcgacct ccgtctggac ggcgaaggcc gtctgggcga cgtccgcgca
     4621 cctcgccgtc ggcggactcc tctggatcgc gggcgtcctc gccgcggtcc tcctcgcccg
     4681 cgccgcggcg ctctcccccg acgcggcggt cgcggaagcc ggcgagccgc aggaggccgc
     4741 cgcgtgaagc gggtcctcgc cgacctcctc gagctgacga agccgcggat cacggtcttc
     4801 gtcgtcctca ccgccttcct cggcttcgcc gccgggacgg acggcccgct cgccgggatc
     4861 gacccgctcc tcctcctcca cacgctcctc ggcacggccc tcgtcgcttc cggcacgagc
     4921 gccttcaacg agatcgccga ggtcgacctc gaccgccgca tggcgcgcac ggcgacgcgg
     4981 ccgctcccgg cgggtcggct tccgttcggg ggcgccgtcg ccttcgcgac gctcctttcg
     5041 ctcctcggcg tcgcggagct ctggctcttc gcgaacggcg tcaccgcctt cctcgcggcc
     5101 ctcacgctga cgagctacgt cttcgtctac accccgatga agacccgctc tcccctctcg
     5161 acgatcgtcg gcgccgttcc cggcgccctc ccgccgctcg gcggctacac ggccgcggcc
     5221 ggcgcgatcg gcgcgccggg cctcgccctc ttcgcgatcc tcttcctctg gcagatgccg
     5281 cacttctacg cgatcggctg gcgccaccgg gaggactacg gcgccgccgg tttccgcatc
     5341 ctctcgaccg tcgacccgac ggggcggagc tccgcgcggc acgccctcgc gttcggcctc
     5401 ctcctcgtcc cggtgagcgt cctcccgacg gccctcggga cggccggcct cgtctacgcg
     5461 gccggtgcgt tcctcctttc ggcgtggttc gcggggacgg cggtgacgtt cttccgcgag
     5521 acgaccgacc gggccgcgaa gtcgatgttc ctcgcttcca tcgcctggct cccggcgatc
     5581 gtcgtcctcc tcctcgccga ccgcgtctcg ggcggatgag cgcgacgccc ctcgtcgagg
     5641 tggaggggtt gagccggagc tacggcgatc ggctcgccct cgacgccgtc tccttcggcg
     5701 tggaggaggg cgagctcttc gccctcctcg gcccgaacgg cggcggcaag acgaccctct
     5761 tcaag
//
LOCUS       DAOTEZ010000275         5765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_9979_length_5765_cov_4.664448, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000275 DAOTEZ010000000
VERSION     DAOTEZ010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9979_length_5765_cov_4.664448"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="P5164_13700"
     CDS             <1..447
                     /locus_tag="P5164_13700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HRY44993.1"
                     /translation="ADEPTTALDVTIQAQLLELLRRLRGELGLAVLLVTHDLDVVAEV
                     ADRALVMYAAEIVEEGPVADLLASPAHPYTRALLAARPTAGSGAENRSLTGLEGSAAV
                     PGAWLSGCRFAPRCPVAVERCGRERPGLSPVARGRAACHLVGRSGT"
     gene            444..1490
                     /locus_tag="P5164_13705"
     CDS             444..1490
                     /locus_tag="P5164_13705"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY44994.1"
                     /translation="MTEPVGRTEALLSVRGLARTFRPPHALLPDRTRREVPAVAGVSF
                     DLVAGETLALVGESGSGKSTTARLVLRLLAPDRGRIDFDGRDWLALPPRELRRARRDV
                     GVVFQDPGRSLNPRMSVGTIVGEPLAIHGIGDAGERRSRVEALLAAVGLPPRSARVRP
                     GELSTGQRQRVAIARALATAPKLVVLDEPVSALDVSVRAQVLNLLLDLQAATPSRPAY
                     LFIGHDLGVVGRVATRTAVMYLGRIVEEGPTGEVLGSPRHPYTALLLASRPGLGNGGA
                     PGAAERRRLAGEPASAATPPPGCPFHPRCPRASERCRTEMPGETTLPGAGGRRYSCHD
                     PEPAAPGNFFPPSS"
     gene            1527..3185
                     /locus_tag="P5164_13710"
     CDS             1527..3185
                     /locus_tag="P5164_13710"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRY44995.1"
                     /translation="MRIDERERRFPRRAAAAALLALAPFWNGAALGSDAGVRVTVPIE
                     SGKRQPQPLPEAERVELTLSQAIELALRNTLDLDVASLTYERSAFGLGAAEGIFDPTL
                     SASVETSSRKSPQTRSFQSDVSKTQSFDLGIGGLTAYGTRYSLGFGGTRSDSPTNTSI
                     PGYVEINPTFSAGLTASVTQPILRGFGKGVNTRLVVQSRLSRDASAWDFVASVQEVVQ
                     AVENAYWDLSYALGNLDAKKEALERAKDFNRITKIKIDVGALAPIEIVQTEVTIAQRE
                     QEIILAEGQIGAAQDQLKRLLNLTDFASWARPIVPVDAPPQEDVALDVESGIRSALEL
                     RPEVKQAINSIESRKVNLAWNRDELRPRLDAKGTYGLSGVGFNQESVRPGSSYSDAIY
                     QVRGADYPNWTLGLSFSVPVFNRTAKSNAAVAATELELAKTNLALLKQNLAVEVRAAA
                     RNVDTAVRSVAAARKARELAERNLDAERKKFDNGMTTSFTVAQVQNDLTSARSNELLA
                     VAGHLKALTAWHKAVGDLLPTKGVELSGMPVAFARTPVEEEARP"
     gene            3182..3949
                     /locus_tag="P5164_13715"
     CDS             3182..3949
                     /locus_tag="P5164_13715"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HRY44996.1"
                     /translation="MTETEAGAAPAAPAGPPEGPSPVGAIAGTFTSPAATFAGLVRRP
                     TWWLPFLLWIAGIAVVVLVSTPKIDMERSFREMFEKRAEKTGQSISDEQIREMAARSE
                     RTPAKAVVWALPFTAATFFLVALLLWGGARASGSEARFGQATAVWAHANLPNVVGLLV
                     SIPLLMSLPDASETQLSVQRILKSNVGAFLPPDAPTFLASIASSIDLFSLAALALLVV
                     GMKKLPSMPPAAGTAVPVVLWGLYVLGKSALAAAFLG"
     gene            3954..4691
                     /locus_tag="P5164_13720"
     CDS             3954..4691
                     /locus_tag="P5164_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY44997.1"
                     /translation="MSTLIEMREIEKTYVVGEEKVRALRGVTFTIGRGEYVAIMGPSG
                     SGKSTLMNLIGCLDTPTGGSYLLNGQLVQDLDDDALARIRNKEIGFVFQTFNLLPRTN
                     ALQQVELPLVYAGLARKERREKAEKALEHVGLTDRAHHNPNELSGGQRQRVAIARALV
                     TNPSILLADEPTGNLDSQTGEDIMKLFRELNGLGNTIVLVTHEEDIAAHAKRIIRIRD
                     GQISDDRPNDSSRAEAFSRAKKVALEA"
     gene            complement(4731..5381)
                     /locus_tag="P5164_13725"
     CDS             complement(4731..5381)
                     /locus_tag="P5164_13725"
                     /inference="COORDINATES: protein motif:HMM:NF003994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HRY44998.1"
                     /translation="MTRAARPGRERLSEAATLRLSLLLRSLARLAEEGTATVSSEALA
                     RRFGMNAAQIRKDLANLGEFGVRGVGYDVADLRSRLASILGLDRARVVVVVGAGHLGQ
                     ALADSGSFNGSSFRVAALFDVDERKVGGRSRTGVPILPAEELARSVAELGAEIGVLAV
                     PAVAAPGAAERLAGAGVKGILNFAPATVGPFDGATVKNVDLALCLESLAVRLSAAG"
     gene            complement(5435..>5765)
                     /locus_tag="P5164_13730"
     CDS             complement(5435..>5765)
                     /locus_tag="P5164_13730"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRY44999.1"
                     /translation="LTLAMLSLTGLPPTVGFIGKLLVFRAAIDAGLMTLALVGIFGSL
                     VSAGYYLRVVYTLWMKEPSREVAAPEEDVLSGAAYILVSVAMLYLGVFPRLLLDLARA
                     AARSLPY"
BASE COUNT          822 a         2153 c         2029 g          761 t
ORIGIN      
        1 gccgacgagc cgacgacggc gctcgacgtc acgatccagg cccagctgct cgagctcctt
       61 cggcgcctgc gaggggagct cggactggcc gtcctcctcg tgacgcacga tctcgacgtc
      121 gtcgcggagg tcgccgaccg cgcgctcgtc atgtacgccg ccgagatcgt cgaggagggg
      181 ccggtcgccg atctcctcgc atctcccgcg cacccgtaca ccagggcgct cctcgccgct
      241 cgcccgacgg ccggcagcgg ggcggagaac cgttccctga cggggctcga aggctcggcg
      301 gccgtccccg gcgcgtggct ctcggggtgc cggttcgccc cccgctgccc ggtcgccgtc
      361 gagcgctgcg gccgcgagag acccggcctg tcgcccgtcg cgagaggccg agccgcgtgt
      421 cacctcgtcg ggcggagcgg cacgtgacgg agcccgtcgg tcgcacggaa gcgctcctct
      481 ccgtgcgggg gctcgcccgg accttccgcc cgccgcacgc ccttcttccg gaccggacgc
      541 gtcgggaggt ccccgccgtc gcgggcgtct ccttcgacct cgtcgccggc gagacgctcg
      601 cgctcgtcgg cgagtcgggc tccggaaaga gcaccacggc ccggctcgtg ctgcgcctgc
      661 tcgcgccgga ccggggccgg atcgacttcg acgggcgcga ctggctcgcc cttccgccgc
      721 gcgagctccg acgggcgcga cgggacgtcg gcgtcgtctt ccaggacccg ggacgctccc
      781 tgaaccctcg catgagcgtc ggcaccatcg tgggcgagcc gctcgcgatc cacggaatcg
      841 gcgacgccgg cgagcgccgg tcccgcgtgg aggcgctcct cgccgccgtc ggccttcctc
      901 cgcggtcggc ccgcgtgagg ccgggcgagc tctccaccgg ccagcgacag cgggtcgcga
      961 tcgcccgcgc cctggccacg gcgcccaagc tcgtcgtcct cgacgagccc gtctcggcgc
     1021 tcgacgtctc ggtccgggcg caggtcctga accttctcct cgacctccag gccgcgacgc
     1081 cgtcccgccc cgcgtacctc ttcatcggtc acgacctcgg cgtcgtcggg cgggtcgcga
     1141 cgcggacggc cgtgatgtac ctgggccgga tcgtcgagga ggggccgacc ggcgaggtcc
     1201 tcggctcgcc gagacacccc tacacggccc ttctgctcgc ctcccggccc ggcctcggga
     1261 acggcggcgc cccgggcgcc gccgaacggc gccgtctcgc cggagagccg gcctccgcgg
     1321 cgaccccgcc ccccggatgt ccgttccatc cccgctgtcc ccgcgcgtcg gagcgctgcc
     1381 ggacggagat gccgggcgag acgaccctgc ccggcgcagg cggccggcgg tattcgtgtc
     1441 acgatccgga gccggccgcc ccgggaaact ttttcccccc ttcttcgtaa gggattggcg
     1501 tggatacacc tggcaaggag gacgccttga gaatcgacga acgggagcgc cgcttcccgc
     1561 gccgcgcggc cgcggccgcg ctcctcgccc tcgcgccgtt ctggaacggc gccgccctcg
     1621 gcagcgacgc gggggttcgc gtcaccgtgc cgatcgagag cggcaagagg cagccgcagc
     1681 cgctccccga ggcggagagg gtcgagctga cgctctccca ggcgatcgag ctcgcgctcc
     1741 ggaacaccct cgacctcgac gtcgcatcgc tcacctacga gcgctcggcg ttcgggctcg
     1801 gagcggccga ggggatcttc gacccgaccc tctcggcgtc ggtcgagacg agctctcgga
     1861 agtcgccgca gacgcgcagc ttccagtcgg acgtctcgaa gacgcagagc ttcgacctcg
     1921 ggatcggagg cctcacggcg tacggcaccc gatacagcct cggattcggc gggacgcgct
     1981 ccgactcgcc gacgaacacc tcgatcccgg gctacgtcga gatcaacccg acgttttccg
     2041 ccggcctcac ggcctccgtc acgcagccga tcctgcgcgg cttcgggaag ggcgtgaaca
     2101 cgcgtctcgt cgtgcagagc cgcctctccc gcgacgcgtc cgcctgggac ttcgtcgcga
     2161 gcgtccagga agtggtccag gcggtcgaga acgcctactg ggacctctcc tacgccctcg
     2221 gcaacctcga cgcgaagaag gaggccctgg agcgcgcgaa ggacttcaac cggatcacga
     2281 agatcaagat cgacgtcggc gccctcgccc cgatcgagat cgtccagacc gaggtgacga
     2341 tcgcccagcg cgagcaggag atcatcctgg ccgaggggca gatcggcgcg gcgcaggacc
     2401 agctcaagcg gctcctgaac ctgaccgact tcgcctcctg ggcccgcccc atcgtcccgg
     2461 tcgacgcgcc cccgcaggag gacgtcgcgc tcgacgtcga gtcgggcatc cgctccgcgc
     2521 tggagctgcg gcccgaggtg aagcaggcga tcaactcgat cgagtcgcgg aaggtgaacc
     2581 tcgcctggaa ccgggacgag ctccggccgc gcctcgacgc gaagggcacc tacggcctct
     2641 cgggcgtcgg gttcaaccag gagtcggtcc ggcccggctc gagctactcc gacgccatct
     2701 accaggtgcg gggcgccgac tacccgaact ggacgctcgg tctgagcttc tcggtcccgg
     2761 tcttcaaccg gacggcgaag tcgaatgccg cggttgcggc gacggagctc gagctggcga
     2821 agacgaacct cgcgctcctg aagcagaacc tcgccgtcga ggtgcgcgcc gccgctcgga
     2881 acgtcgacac ggcggtccgc tccgtggccg ccgcccgcaa ggcgcgagag ctcgccgagc
     2941 ggaacctcga cgccgagcgg aagaagttcg acaacgggat gacgacctcc ttcacggtcg
     3001 cccaggtcca gaacgacctg acctcggccc gctcgaacga gctcctcgcc gtcgcggggc
     3061 acctgaaggc gctcaccgcc tggcacaagg cggtggggga cctcctcccg acgaagggtg
     3121 tcgagctgag cgggatgccg gtggccttcg cgcggacgcc ggtcgaggaa gaggcccggc
     3181 cgtgaccgag accgaggccg gcgccgcccc cgcggcgccg gcgggcccgc ccgagggccc
     3241 ttctcccgtc ggcgcgatcg ccggcacctt cacgagcccc gcggcgacgt tcgcggggct
     3301 cgtccgccgc ccgacctggt ggctcccgtt cctcctctgg atcgccggga tcgcggtcgt
     3361 cgtcctcgtc tcgacgccca agatcgacat ggaacgctcc ttccgggaga tgttcgagaa
     3421 gcgggccgag aagacgggcc agtcgatctc ggacgagcag atccgcgaga tggccgcgcg
     3481 ctccgagagg acgcccgcga aggccgtcgt ctgggcgctc ccgttcacgg cggccacgtt
     3541 cttcctcgtc gccctcctcc tgtggggagg ggctcgggct tcgggctcgg aggcccggtt
     3601 cgggcaggcg acggccgtct gggcccacgc gaacctgccg aacgtcgtcg ggctgctcgt
     3661 ctcgatcccg ctcctgatgt cgcttcccga cgcctcggag acccagctct cggtccagag
     3721 gatcctgaag tcgaacgtgg gggccttcct ccctcccgac gctcccacgt tcctcgcgtc
     3781 gatcgcctcc tccatcgacc tcttcagcct cgcggcgctc gccctcctcg tcgtcgggat
     3841 gaagaagctg ccgtcgatgc cgcccgccgc cgggacggcc gtcccggtcg tcctgtgggg
     3901 cctctacgtg ctcggaaagt cggccctcgc ggccgcgttc ctggggtgac gccgtgtcga
     3961 cgctgatcga gatgcgggag atcgagaaga cctacgtggt gggcgaggag aaggtccgcg
     4021 ccctccgggg ggtcacgttc accatcggcc gcggcgagta cgtcgcgatc atgggtccct
     4081 cgggctcggg gaagtcgacg ctgatgaacc tcatcggctg cctcgacacg ccgaccggcg
     4141 ggagctacct cctgaacgga cagctcgtcc aggacctcga cgacgacgcc ctcgcgcgga
     4201 tccggaacaa ggagatcggg ttcgtcttcc agaccttcaa cctcctcccg cggacgaacg
     4261 cgctccagca ggtcgagctc ccgctcgtct acgcggggct cgcccgcaag gagcgccgcg
     4321 agaaggccga gaaggcgctc gagcacgtcg ggctgaccga ccgggcccac cacaacccga
     4381 acgagctctc cggcgggcag cggcagcgcg tggcgatcgc gcgcgccctc gtgacgaacc
     4441 cctcgatcct cctcgccgac gagccgacgg ggaacctcga ctcgcagacg ggcgaggaca
     4501 tcatgaagct cttccgggag ctgaacgggc tggggaacac gatcgtcctc gtcacccacg
     4561 aggaagacat cgccgcgcac gcgaagcgga tcatccggat tcgcgacggc cagatctcgg
     4621 acgaccgtcc gaacgactcg tcccgggcgg aggccttctc ccgggcgaag aaggtcgcgc
     4681 tggaggcgtg atccggggcg gcggggcggc ggagacgccg cctcgccgcg tcagccggcc
     4741 gccgagaggc ggacggcgag cgactcgagg cagagcgcca ggtcgacgtt cttcaccgtc
     4801 gcgccgtcga aggggccgac ggtggcgggc gcgaagttga ggatcccctt cacgccggcg
     4861 cccgccagcc tctccgccgc gcccggagcc gccacggccg ggacggcgag cacgccgatc
     4921 tccgcgccga gctcggcgac cgaacgcgcg agctcttcgg cggggaggat cgggaccccg
     4981 gttcgcgatc ggccgccgac cttgcgctcg tcgacgtcga agagcgccgc gacccggaac
     5041 gaggacccgt tgaagctccc ggagtcggcg agcgcctgcc cgaggtgtcc cgcgcccacg
     5101 acgacgacga cgcgggcccg gtcgagcccg aggatcgagg cgagccggct ccgcaggtcc
     5161 gccacgtcgt agccgacgcc gcggactccg aactctccga ggttcgcgag gtccttccgg
     5221 atctgcgcgg cgttcatgcc gaagcgccgc gcgagggcct ccgaggagac cgtggcggtc
     5281 ccctcctccg cgaggcgcgc gaggctccgg aggaggagcg agagccgaag ggtcgccgcc
     5341 tcggagaggc gctcccgccc ggggcgcgcg gcccgcgtca cgggagggcc ctccggggcg
     5401 ctcccgcgac gtcggacccc gcgcgcatcg ccggtcagta ggggagggac cgggcggccg
     5461 cccgggcgag atcgaggagg aggcgcggga agacgcccag gtagagcatc gccaccgaga
     5521 cgaggatgta ggcggcgccg gagaggacgt cctcctcggg cgcggcgacc tcccggctcg
     5581 gctccttcat ccagagggtg tagacgacgc ggaggtagta ccccgcggag acgaggctcc
     5641 cgaagatgcc gaccagcgcg agcgtcatca ggccggcgtc gatggcggcc cggaaaacga
     5701 ggagcttccc gatgaagccg accgtcggcg ggaggcccgt gagcgagagc atcgcgagcg
     5761 tcaga
//
LOCUS       DAOTEZ010000276         5753 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_10004_length_5753_cov_6.597929, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000276 DAOTEZ010000000
VERSION     DAOTEZ010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10004_length_5753_cov_6.597929"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="P5164_13735"
     CDS             complement(<1..499)
                     /locus_tag="P5164_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45000.1"
                     /translation="MHDRIDPRLRRLLARLPLAAGLAILLWFALVGRPWSALVTRASE
                     GWIRAAERTKVTRLAFEDGMVVVQRADLSSRSETPAFSAAPMTANLVLLLALLFATPR
                     DLRAPAYAARVAAAFAALLLTQVLHLSFAVQTLYATQLGAWSVATWPRWQREVVATGR
                     YFFDIV"
     gene            complement(468..1016)
                     /gene="xrtH"
                     /locus_tag="P5164_13740"
     CDS             complement(468..1016)
                     /gene="xrtH"
                     /locus_tag="P5164_13740"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase H"
                     /protein_id="HRY45001.1"
                     /translation="MKSASTTETDPAGRKRLRPEAAFLLKFVLLLLLFFAATAPKPMN
                     DAFVEPFTAGVAKVGGFAAGLFGEETRMEGTVIRSPRFAVNVRTGCNGLETIYIFFAG
                     VLAFPAPWGRKLLGLAGGFLAIQLLNVVRIVSLFYIGVYFPQHFEDSHIVVWQAIVIL
                     FGVALFLLWADRYARPDRPAAP"
     gene            1060..2418
                     /locus_tag="P5164_13745"
     CDS             1060..2418
                     /locus_tag="P5164_13745"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRY45002.1"
                     /translation="MSDPEARRFRRNLTAVLSLVGLLTAGSVFLFGHLLFKTLSKDVV
                     DEALLYSRQDAQRIARGLAERASGDLYTLKVNQEDLDVFVGSALAERRILSEVRVYDS
                     RNQLVYAYSGVLSRPERAGAHPEAGQTISTNPLEPASRSTETTAQIADPYGIEVPIGD
                     FGTLKVGVSQRELEARVEVLRVKLYKRIFAAALVSFLGILAAAWALMLLVRRTRLVEA
                     ARIEAERRAELGEMATGLAHEIRNPLNAMSLNLELLEEQLEKGSSGLSTAELASATRV
                     ETGRLARLLSDFLSYARPSPLVTVPSDLNEPASEAVAFLGPEARTRRIALTFRPWPGG
                     APALLDTARVKQVVLNLVGNALDAVEAQGARAREVDVAVEDGGRFWRLTVSDHGPGVD
                     GKGSGDVFRVFVSSKPAGTGMGLPIAERIVHAHGGELTLVSAAGEPTRAVATFPKHDA
                     PS"
     gene            complement(2586..2894)
                     /locus_tag="P5164_13750"
     CDS             complement(2586..2894)
                     /locus_tag="P5164_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45003.1"
                     /translation="MNPKRIRTALAAALLSLAFLAPAAEAARVVVKVGPPAARAEVRA
                     TAPSPRHVWVGGYWRWDGRAHVWVAGGWQLPPRHRAVWVEGHWKKVRGGWTWMPGHWR
                     "
     gene            3037..3633
                     /locus_tag="P5164_13755"
     CDS             3037..3633
                     /locus_tag="P5164_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRY45004.1"
                     /translation="MATDVGLVVRNLLDFYPFAGKTVVSVGAGGGQLVEYGRAARRVV
                     AVDGDGEALGRLGDVLAAKGLAGRFELVCSDVLEVSARGDVVLFEFSLHEMPDPERAL
                     AHARTLASDVVVLDHAPGSGWAWHVVEEEKVKRSAEAMRRLGVRAERRFLGEQRFADH
                     AELLAKVSVQGPLAVARAERFRGAGEIVIPFGYEVALL"
     gene            complement(3648..>5753)
                     /locus_tag="P5164_13760"
     CDS             complement(3648..>5753)
                     /locus_tag="P5164_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HRY45005.1"
                     /translation="GRVYVSTYPTMMGLIDETNGGERRFGPGHFDLVVIDEAHRSVYR
                     KYGAIFDYFDSLLVGLTATPKDEIDRDTYRLFDLDRGVPTDAYPLEEAVKDGFLVPAR
                     AVSVPLKFPRQGITYADLTEEEREEWDAVDWSDDGTTPARVEAAAVNKWLFNADTVDR
                     VLAHLMARGQKVAGGDRLGKTIVFAANQDHAEFIAERFDAGWPHLKGHFARVVHCGLP
                     YAQSLIDAFSSPSKAPHVAISVDMLDTGIDVPEVVNLVFFKLVRSKTKFWQMVGRGTR
                     LSPGLFGPGQDKREFFIFDYCGNLEFFALDPPATDGAVGDPLGKRLFVRRLDLIGELD
                     ERLAPAPTTPGSSPIDETNLASRRADDAALRGEVAALLHAEVSSMSLESFVVRPKRRL
                     VEKYREPGAWTELSNVARQELATEVAGLPSGLAPEEEEAKRFDLLMLHLQLSVLRHDP
                     SFLRLSEPVRAICGLLEEKAAIPMVAERIVLIQAMQSDEWWQDVTPTMLESARKHLRG
                     LVSLIEKRRRAPVYTDFTDEMGRERLVDLPGFGAPDGFERFREKARAFLREHEDHVTI
                     RKLRSNRPLTRSDLDELERMLGETGVGDALSIEKAKKESEGLGLFVRSLVGLDREAAK
                     AAFGGFLDGRTLNASQIEFVDLIVNHLTEHGVMSPALLYESPFTDLAPRGPDGLFPSS
                     QVDELVAILGRVRAAAIAA"
BASE COUNT          806 a         2011 c         2133 g          803 t
ORIGIN      
        1 cgacgatgtc gaagaagtag cgccccgtcg cgacgacctc cctctgccag cgcggccacg
       61 tcgcgacgct ccaggcgccg agctgcgtcg cgtagagcgt ctggacggcg aaggagaggt
      121 gcagcacctg ggtcaggagg agcgcggcga aggcggcggc gacccgggct gcgtacgcgg
      181 gcgcgcgcag gtcgcgcgga gtcgcgaaga gcagcgcgag caggaggacg aggttcgccg
      241 tcatcggcgc agccgaaaag gccggcgtct cggaccggga ggagaggtcg gccctctgca
      301 cgacgaccat tccgtcctcg aacgcgaggc gggtcacctt cgtccgctcg gcggcgcgga
      361 tccaaccctc ggacgcgcgc gtgacgagcg cgctccaggg acggccgacg agcgcgaacc
      421 agaggaggat cgcgagcccc gccgcgagcg gcagccgcgc gaggagccta cggagccgcg
      481 ggtcgatccg gtcgtgcata gcggtccgcc cagaggagga agagggcgac gccgaagagg
      541 atgacgatcg cctgccagac gacgatgtgc gagtcctcga agtgctgcgg gaagtagacc
      601 ccgatgtaga agaggctgac gatcctcacg acgttcagga gctggatggc gaggaacccg
      661 ccggcgaggc ccaggagctt gcggccccac ggggcgggga aggcgaggac gccggcgaag
      721 aagatgtaga tcgtctcgag gccgttgcag cccgtccgga cgttgacggc gaagcggggc
      781 gagcggatca cggtcccctc catccgcgtc tcctcgccga agaggccggc cgcgaagccc
      841 cccaccttcg cgacgcccgc cgtgaacggc tcgacgaagg cgtcgttcat cggcttcggc
      901 gcggtggcgg cgaagaagag gaggaggagg aggacgaact tcaggaggaa ggccgcttcg
      961 gggcgcaggc gcttccttcc ggccgggtcg gtctctgtcg tggaggcgct cttcacggcg
     1021 aggcgggagg atagcgcacg cgcacgtaaa ctccgggccg tgtccgaccc cgaggcgcgc
     1081 cgctttcgcc ggaacctcac cgccgtcctc tccctcgtcg gcctgctgac cgctgggtcg
     1141 gtcttcctct tcgggcacct cctcttcaag acgctctcga aggacgtcgt cgacgaggcg
     1201 ctcctctact cccgccagga cgcgcagcgg atcgcccgcg gcctcgccga gcgggcgtcg
     1261 ggcgacctct acacgctgaa ggtcaaccag gaggacctcg acgtcttcgt cggctccgcc
     1321 ctcgcggagc gtcgcatcct ctccgaggtc cgcgtctacg actcgcggaa ccagctcgtc
     1381 tacgcctact ccggagtcct ctcgcggccc gagcgcgccg gcgcgcaccc ggaggcgggg
     1441 cagacgatct cgacgaaccc gctcgagccc gcctcgcgct ccaccgagac gaccgcacag
     1501 atcgccgacc cgtacggcat cgaggtcccg atcggcgact tcggcacgct gaaggtcggc
     1561 gtctcgcagc gggagctcga ggcgcgcgtc gaggtcctcc gggtgaagct ctacaagcgg
     1621 atcttcgccg cggcgctcgt ctccttcctc ggcatcctcg ccgccgcctg ggcgctgatg
     1681 ctcctcgtcc gccggacgcg gctcgtcgag gcggcgcgca tcgaggccga gcggcgggcc
     1741 gagctggggg agatggcgac cggcctcgcg cacgagatcc gcaatcccct gaacgccatg
     1801 agcctcaacc tcgagctcct cgaggagcag ctcgagaagg gctcctcggg cctctccacc
     1861 gccgagctcg cctccgccac gcgggtggag accgggcgcc tcgcgcgcct cctctccgac
     1921 ttcctctcct acgcccgccc ctcgccgctc gtcacggtgc cgtccgacct gaacgagccg
     1981 gcgtcggagg cggtcgcgtt cctggggccc gaggcgcgga cgcgccgcat cgcgctcacg
     2041 ttccgtccgt ggccgggcgg cgccccggcg ctcctcgaca cggcgcgggt caagcaggtc
     2101 gtcctgaacc tcgtcggcaa tgcgctcgac gccgtggagg cccagggggc ccgggcgcgc
     2161 gaggtcgacg tggcggtcga ggacggcgga cgcttctggc gcctcaccgt ctccgaccat
     2221 ggccccggcg tcgacggcaa ggggagcggc gacgtcttcc gggtcttcgt ctcgtcgaag
     2281 ccggccggga ccggcatggg gctcccgatc gcggagcgga tcgtccacgc gcacggcggc
     2341 gagctgaccc tcgtttctgc ggccggggag ccgacccgcg ccgtggcgac gttcccgaag
     2401 cacgacgctc cgagctgagc ctgcgccgcg cggtgcgcct cctccttttc ggtcggggtc
     2461 tcccgagcgc aacgacgccc gccggctcgg atcgagaccg gcgggcgttc cgggggggcc
     2521 gcccggggaa gctgccgggg gaggccgcct caccccgatt ctcctccttt tcggtccgag
     2581 cgcgctcacc tccagtggcc gggcatccag gtccagccgc cgcggacctt cttccagtgt
     2641 ccctcgaccc agacggcccg gtgccggggc gggagctgcc agccgcccgc gacccagacg
     2701 tgcgcccggc cgtcccagcg ccagtagccg ccgacccaga cgtggcgcgg cgacggggcg
     2761 gtcgcccgaa cctccgcgcg cgccgccggc gggccgacct tgaccacgac gcgggccgcc
     2821 tccgcggcgg gggcgaggaa ggcgagagag agaagagcgg cggcgagagc ggtgcggatg
     2881 cgcttcgggt tcatcgtcgg gtcctccttc gagtggcgtc tcgagggatg gacgccgacg
     2941 gcgtgcggcc ggttggcgac ggacggcagg gcggtgaggg aggtcacgaa gagccggccc
     3001 gaagcgtccc ggcgcggcct gtgcgagtct cgagggatgg cgaccgacgt cggcctcgtc
     3061 gtccggaacc tcctcgactt ctacccgttc gccgggaaga ccgtcgtctc cgtcggggcg
     3121 ggcgggggcc agctcgtcga gtacgggcgc gcggcgcgcc gggtcgtcgc cgtcgacggg
     3181 gacggcgagg ccctcgggcg cctcggcgac gttctcgcgg cgaaggggct cgctgggagg
     3241 ttcgagctcg tctgctccga cgtcctcgaa gtctccgccc gcggcgacgt cgtcctcttc
     3301 gagttctccc tccacgagat gcccgatccg gagagggccc tcgcgcacgc caggaccctc
     3361 gcgtccgacg tcgtcgtcct ggaccacgca cccggctccg ggtgggcctg gcacgtcgtc
     3421 gaggaggaga aggtgaagcg gagcgcggag gcgatgcgac gcctcggggt ccgcgcggag
     3481 cggcgcttcc tcggcgagca gcgtttcgcc gaccacgccg agctcctcgc gaaggtctcg
     3541 gtccaggggc ctctcgccgt cgcgcgggcg gagcggttcc gcggggcggg ggagatcgtg
     3601 atcccgttcg ggtacgaagt cgccttgctc tgaggtccgg ggcgcgccta ggccgcgatg
     3661 gccgccgcgc ggacccggcc caggatcgcc acgagctcgt cgacctgcga cgacgggaag
     3721 aggccgtcgg ggccgcgggg ggcgaggtcg gtgaaggggg actcgtagag gagcgcgggg
     3781 ctcatgacgc cgtgctcggt caggtggttc acgatcaggt cgacgaactc gatctggctc
     3841 gcgttcaggg tccggccgtc gaggaagcct ccaaaggcag ccttggcggc ctcccggtcg
     3901 aggccgacga gagagcggac gaagaggccg agcccctcgc tctccttctt cgccttctcg
     3961 atggagagcg cgtcgccgac gccggtctcc ccgagcatcc gctccagctc gtcgaggtcg
     4021 gagcgggtga ggggccggtt cgagcggagc ttccggatcg tcacgtggtc ctcgtgctcg
     4081 cggaggaagg cgcgcgcctt ctcgcggaag cgctcgaagc cgtcgggggc gccgaagccg
     4141 gggaggtcga cgagccgctc gcgccccatc tcgtcggtga agtcggtgta gacgggggcg
     4201 cgccgccgct tctcgatgag cgagacgagg ccgcgaaggt gcttgcgggc cgactcgagc
     4261 atcgtcggcg tcacgtcctg ccaccactcg tccgactgca tcgcctggat caggacgatc
     4321 cgctcggcga ccatcgggat cgcggccttc tcctcgagga ggccgcagat cgcccggacc
     4381 ggctcgctca ggcgcaggaa ggacgggtcg tggcggagga cggagagctg gaggtggagc
     4441 atcaggaggt cgaaccgctt cgcctcctcc tcctcgggcg cgaggccgct cgggaggccc
     4501 gcaacctcgg tggccagctc ctgccgggca acgttcgaga gctccgtcca ggccccgggc
     4561 tcgcggtact tctcgacgag ccggcgcttc ggccggacga cgaagctctc gaggctcatc
     4621 gaggagacct cggcgtggag gagcgccgcc acctcgccgc ggagcgcggc gtcatcggcg
     4681 cggcggctcg cgaggttcgt ttcgtcgatc gggctcgagc ccggtgtcgt cggggcgggc
     4741 gcgaggcgct cgtccagctc gccgatcagg tcgaggcggc ggacgaagag cctcttcccg
     4801 agcgggtcgc ccacggcgcc gtccgtcgcg ggcgggtcga gagcgaagaa ctccaggttc
     4861 ccgcagtagt cgaaaatgaa gaactcccgc ttgtcctgcc cggggccgaa gaggccgggg
     4921 gagaggcgcg tcccccggcc gaccatctgc cagaacttcg tcttcgacct cacgagcttg
     4981 aagaagacga ggttcacgac ctcggggacg tcgatccccg tgtcgagcat gtcgaccgag
     5041 atcgcgacgt gcggggcctt cgacgggctc gagaaggcgt cgatgaggct ctgggcgtag
     5101 gggaggccgc agtggacgac gcgggcgaag tgccccttca ggtgcggcca gcccgcgtcg
     5161 aagcgctccg cgatgaactc ggcgtggtcc tggttcgcgg cgaagacgat cgtctttccg
     5221 agccggtcgc cgccggcgac cttctgcccg cgggccatca ggtgcgcgag gacccggtcg
     5281 accgtgtcgg cgttgaagag ccacttgttc acggccgcgg cctcgaccct cgcgggcgtg
     5341 gtgccatcgt cgctccagtc gacggcgtcc cactcctccc gctcctcctc cgtcaggtcg
     5401 gcgtaggtga tcccctgccg cgggaacttc agcggcaccg agacggcccg cgccgggact
     5461 aggaagccgt ccttcacggc ctcctcgagc gggtaggcgt cggtcgggac gccgcggtcg
     5521 aggtcgaaca ggcggtaggt gtcgcggtcg atctcgtcct tcggcgtcgc cgtgaggccg
     5581 acgaggaggg agtcgaagta gtcgaagatc gcgccgtact tccggtagac ggagcggtgc
     5641 gcctcgtcga tgacgacgag gtcgaagtgc cccggcccga accgccgctc gcccccgttc
     5701 gtctcgtcga tgagccccat catcgtcggg taggtcgaga cgtagacgcg ccc
//
LOCUS       DAOTEZ010000277         5753 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_10005_length_5753_cov_5.240435, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000277 DAOTEZ010000000
VERSION     DAOTEZ010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10005_length_5753_cov_5.240435"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="P5164_13765"
     CDS             complement(<1..121)
                     /locus_tag="P5164_13765"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569767.1"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRY45006.1"
                     /translation="MSEPAKKTVLSGLRPTGRVHLGNYWGAVKNWVDLQDRYEC"
     gene            complement(118..792)
                     /locus_tag="P5164_13770"
     CDS             complement(118..792)
                     /locus_tag="P5164_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRY45007.1"
                     /translation="MPDAPVSPEKVLEIVIQVAALLFAVSVHESAHGWMALRCGDTTA
                     RDLGRITLNPLKHVDPFGSLLVPALLAFTGAPVFGWAKPVPVSLRGVRNPRRANLLVS
                     AAGPISNFAVAVLAAVAFFVLRAVAPPGAELRTSILYPLLLVALFSVAVNVSLGIFNL
                     VPIPPLDGFGVVESFAPARWIRGVMWVRRYGFVLLVVAIFTGALSAVLRPPMRFVFQL
                     LLGGPS"
     gene            complement(820..1194)
                     /locus_tag="P5164_13775"
     CDS             complement(820..1194)
                     /locus_tag="P5164_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRY45008.1"
                     /translation="MELSLETDGSVAALKVRGDVTLTEASTITEYLRVARENGALRCV
                     VDLSACTALPTTIVAVLMREQGRFASSGGTLALAGVGEQNPFLAQSVAAGRFGHYRTV
                     EEGLAAERRASGTPFDAPSGGT"
     gene            complement(1236..3344)
                     /gene="recG"
                     /locus_tag="P5164_13780"
     CDS             complement(1236..3344)
                     /gene="recG"
                     /locus_tag="P5164_13780"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRY45009.1"
                     /translation="MPRPARGREPFDPGTALDGLPGIGPARSRALAEAGLATVLDLLL
                     ELPVRHEDRARFARVADLVAGGPPLTLFGTITAARLVRTRRRGFSIYEATLEDESGSV
                     DLAFFSQPWLARWLTPGARAFVHGRAVWKRRLVLESPHVEAEPEDPDDAALSVGRVVP
                     IYRSLPGLPPRLRRRLVARALTEAAPKLPDRLRPEDAAALDLPPLAEALREAHFPGIA
                     GGPPDPRPFRERTSRHLRRLAFEELLELQTALARRRREREARPAPRIEADSSVRKALA
                     AMLPFPLTASQKQAIREIGADLRSGHPMARLLQGDVGSGKTIVAVLTAVLAARRGFQT
                     ALLAPTTLLAEQHAETAHRLLSGSGVTPALLTGRVTGRPRAALLSALSEGEIGLLVGT
                     HALLERPVAFRSLGLVVVDEQHRFGVAQRAALPARTSARREHPHVLVLSATPIPRSLA
                     LALHGDLDVSTLTEKPPGRTPVATAVVDEGRREEAYRSLAADVGAGGRAYVVVPLVEE
                     SETIEGRSVAAWTEEVARRLAGRRVGALHGKMRAEARETAMRRFAAGEIDVLVATTVV
                     EVGIDVPEASFLLVENAERFGLAQLHQLRGRVGRGARASTCVLVAGSGASPEALSRLA
                     VLAGTDDGFAVAEEDLRRRGPGEALGTRQSGLPGLRIADPIADRDLLERAKAFASALA
                     ARGAAAPFEERLILRPGDPR"
     gene            complement(3347..4129)
                     /locus_tag="P5164_13785"
     CDS             complement(3347..4129)
                     /locus_tag="P5164_13785"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRY45010.1"
                     /translation="MKVVLKSGGGAASFEEYIVRVPAGETVFSEGDVGTEMYVIQSGT
                     VDIVKKVKDEARVLSVLERGDFFGEMSILEDVPRTADAIARTDVELVRINQSTFDEML
                     RHNGEIAVRMLRKLSRRLRETTALLEQTTGEAPALDESSDFFERTGARDGIFRLVADG
                     GVEEFYLNRDTETLVGRIDPVTGIRPDVDLTNLDGPRSVSRRHAKIVRIDGEFHVIEE
                     IGTMNGTFVNGNRIATGAPVPIHDGDRLRFGLVDLTFRVTRS"
     gene            complement(4126..5061)
                     /locus_tag="P5164_13790"
     CDS             complement(4126..5061)
                     /locus_tag="P5164_13790"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HRY45011.1"
                     /translation="MSAPLRRPRRLRPGSLVGVAALSGPVRPESLDAGVAALRGFGYR
                     VRVARNVLETEPLLGLAGGDEARVSGYVALVEDPEVEAILFSRGGYGVTRLLHRLPER
                     LLADTAKLHCGFSDLTALAAFLRERCRLVSIHGPMVAADLSAPLDAWTAPSFPAVLEG
                     RGPDRLEVPSADVLVPGRAEGPLVGGCLSLLAALCGTPWDYDYAGALLFFEEVGEEAY
                     RVDRMLGTLVSSGRWGRLAGIIVGSMTRVTFGGSEDPDRLRQLLFDRLAPLGVPVALG
                     LPFGHGGANVPLPVGTRASWDGEARTLAFEEESLS"
     gene            complement(5058..5510)
                     /locus_tag="P5164_13795"
     CDS             complement(5058..5510)
                     /locus_tag="P5164_13795"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRY45012.1"
                     /translation="MADLIDGNNLLGQKGISRETLVKELAELSRAKRRRLTVVFDGPP
                     EHGRPKVQQLGDVTVVYAAPRSADEEIVRRVREARDPRGVTVVTDDRVLSESVDAAGG
                     RTLPVAAFRQTGARRLTAKAARDEEKRDPGGSASDWARWFSDPRNRIG"
BASE COUNT          846 a         2088 c         2122 g          697 t
ORIGIN      
        1 ggcactcgta ccggtcctgc aggtcgaccc agttcttcac cgcgccccag tagtttccga
       61 ggtggacacg ccccgtgggc ctgaggcccg agaggacggt tttcttcgcc ggttcgctca
      121 agagggacct ccgagtagga gctggaagac gaatcgcatc ggcggcctca ggacggccga
      181 gagcgccccc gtgaagatcg ccacgacgag gaggacgaac ccgtatcgcc gcacccacat
      241 cacgccccgg atccatcggg cgggcgcgaa gctctcgacg accccgaagc cgtcgagcgg
      301 cgggatcggg acgaggttga agatgccgag agagacgttc acggccaccg agaacagcgc
      361 cacgaggagc agcgggtaga ggatcgacgt ccgcagctcg gcgcccggcg gcgcgacggc
      421 gcggaggacg aagaacgcga ccgccgcgag gaccgccacg gcgaagttcg agatcggccc
      481 cgcagccgag acgaggaggt tcgcccgccg cgggttccga acgccgcgga gcgagaccgg
      541 gaccggcttg gcccagccga agaccggcgc gcccgtgaac gcgaggagcg caggcacgag
      601 gaggctcccg aacgggtcga cgtgcttcag ggggttcagc gtgatccggc cgaggtcgcg
      661 cgccgtggtg tcgccgcagc gcaacgccat ccagccgtgc gcggactcgt gcacggagac
      721 cgcgaacagg agggcggcca cctggatgac gatctcgagg accttttcgg gggagacggg
      781 cgcgtcgggc acggcaggaa cgtccgaaaa cgcccgatcc tacgtgcccc ccgagggggc
      841 gtcaaacggc gtgccggacg cgcgccgctc ggcggcgagc ccttcctcga cggttcgata
      901 gtggccgaaa cgcccggcgg ccacgctctg cgcgaggaac gggttctgct cgcccacgcc
      961 cgccagggcg agcgtcccgc ccgacgaggc gaaacgcccc tgctcgcgca tgaggacggc
     1021 gacgatcgtc gtcgggagag ccgtgcaggc ggaaaggtcg accacgcagc gcagggcgcc
     1081 gttctcgcgg gcgacgcgga ggtactccgt gatcgtcgac gcctccgtga gcgtcacgtc
     1141 gccccggact ttcaacgcgg cgaccgatcc gtcggtctcg agggaaagct ccacacccgg
     1201 aggatgccac agcgccgcgc gagcgcgccc cttcgctacc gcgggtcgcc cggccggagg
     1261 atcagccgct cctcgaaggg agccgcggcc ccgcgagccg cgagcgccga ggcgaaggcc
     1321 ttcgcccgct cgaggaggtc cctgtcggcg atcgggtcgg cgatccgcag gccgggcagg
     1381 ccgctctgac gcgtgccgag cgcctccccg ggtccgcgcc ggcgaaggtc ctcctctgcg
     1441 acggcgaacc cgtcgtccgt ccccgcgagg accgcgaggc gcgacagggc ctcgggcgag
     1501 gccccggacc ccgcgacgag gacgcaggtc gaagcccgcg caccccgccc cacgcggccg
     1561 cggagctggt ggagctgtgc gaggccgaag cgctccgcgt tctccacgag gaggaacgac
     1621 gcttcgggga cgtcgatgcc cacctcgacg accgtcgtgg cgacgaggac gtcgatctct
     1681 ccggcggcga agcggcgcat cgccgtctcc cgcgcctcgg cccgcatctt cccgtggagg
     1741 gcgccgacgc ggcggccggc gaggcgccgc gccacctcct ccgtccaggc ggcgaccgat
     1801 cgcccctcga tcgtctcgga ctcctcgacg agcggcacga cgacgtacgc gcgccctccc
     1861 gctccgacgt cggccgcgag agagcggtac gcctcctcgc gccgcccctc gtccacgacg
     1921 gccgtcgcga ccggcgtccg gcccgggggc ttctcggtga gcgtcgagac gtcgaggtcc
     1981 ccgtggagcg cgagcgcgag ggaacggggg atcggcgtgg ccgacaggac gaggacgtga
     2041 gggtgctcgc ggcgcgccga ggtccgtgcg gggagcgcgg cgcgctgcgc taccccgaac
     2101 cgatgctgct cgtcgacgac gacgagcccc aggctgcgaa atgcgacggg cctctcgagg
     2161 agcgcgtgcg ttccgacgag gaggccgatc tccccttccg agagcgccga gaggagggcc
     2221 gcccgcgggc gtccggtcac gcgccccgtc agcagcgccg gcgtcacgcc gctgccggag
     2281 aggagcctgt gggcggtctc cgcgtgctgc tcggcgagga gggtcgtcgg cgccagcagg
     2341 gcggtctgga agccgcgccg cgccgcgagg acggccgtca ggacggcgac gatcgtcttg
     2401 ccgcttccga cgtcgccctg gaggaggcgc gccatcgggt gccccgaacg caggtcggct
     2461 ccgatctctc ggatggcctg cttctgcgat gccgtcagcg ggaacggaag catcgcggcg
     2521 agagccttcc gaaccgagga gtcggcctcg atccggggcg cgggccgggc ctccctctct
     2581 ctccggcgcc gcgcgagcgc ggtctggagc tcgagaagct cctcgaaggc gagacggcgg
     2641 aggtggcgcg aggtgcgctc gcggaacggc cgggggtcgg gcggacctcc cgcgatcccg
     2701 ggaaagtgcg cttcgcgaag ggcctctgcg agagggggaa ggtcgagcgc ggccgcgtcc
     2761 tccggacgga gacgatccgg cagcttcggc gcggcctccg tgagggcgcg cgccacgagc
     2821 ctccgccgga gccggggcgg gagacccggg agggacctgt agatcgggac gacccgcccc
     2881 acggagagcg ccgcgtcgtc cgggtcttcg ggctccgcct cgacgtgggg cgactcgagg
     2941 acgagcctgc gcttccagac ggcgcgtccg tggacgaagg cgcgcgcgcc gggcgtcagc
     3001 cagcgcgcga gccagggctg agagaagaac gccaggtcga ccgagccgct ttcgtcctcg
     3061 agcgtcgcct cgtagatcga gaagccgcgc cggcgcgttc ggacgaggcg ggccgcggtg
     3121 atcgtcccga acagcgtcag cggcggcccg ccggcgacga ggtccgccac gcgggcgaac
     3181 cgggcccggt cctcgtggcg gaccggcagc tcgaggagga ggtcgaggac ggtcgcgagc
     3241 cccgcctcgg cgagggctcg cgaccgggcc ggtccgatgc cggggagccc gtccagcgcc
     3301 gttccgggat cgaagggctc ccggccgcgc gcggggcgcg gcacgctcag ctccgggtga
     3361 cccggaacgt caggtcgacg aggccgaagc gaagccggtc gccgtcgtgt atcgggacgg
     3421 gggcgccggt ggcgatccgg ttcccgttca cgaacgtgcc gttcatcgtc ccgatctcct
     3481 cgatcacgtg gaactccccg tcgatgcgga cgatcttcgc gtggcggcgg gagaccgagc
     3541 gcgggccgtc caggttcgtc aggtcgacgt cgggccggat cccggtcacc gggtcgatcc
     3601 tcccgacgag ggtctccgtg tcgcggttca ggtagaactc ctcgacccct ccgtccgcga
     3661 cgagccggaa gatcccgtcc cgcgcacccg tccgctcgaa gaagtcggaa gactcgtcga
     3721 gggcgggggc ctcgcccgtc gtctgctcga gcagcgcggt cgtctcccgc agacgccggg
     3781 agagcttccg gagcattcgc acggcgatct cgccgttgtg ccggagcatc tcgtcgaacg
     3841 tcgactggtt gatccggacg agctcgacgt ccgtccgtgc gatggcgtcc gcggtccggg
     3901 ggacgtcctc gaggatcgac atctctccga agaagtcgcc cctctcgagg acggagagga
     3961 cgcgtgcctc gtccttgacc ttcttcacga tgtcgaccgt ccccgactgg atcacgtaca
     4021 tctcggtccc gacgtctcct tccgagaaga ccgtctctcc ggccgggacc cgtacgatgt
     4081 actcctcgaa gctcgcggcg ccgccgccgg acttcagcac gactttcacg agagggactc
     4141 ctcctcgaag gcgagcgtcc gcgcctcgcc gtcccacgat gcgcgcgttc cgacgggaag
     4201 cggcacgttc gcccccccgt gtccgaacgg caggccgagg gcgaccggga ccccgagcgg
     4261 ggcgagccgg tcgaagagga gctgccggag gcggtccggg tcctcgctgc cgccgaacgt
     4321 cacacgcgtc atggagccca cgatgatacc ggcgagccgg ccccatcggc ccgaagaaac
     4381 gagggtcccg agcatacggt cgacccggta cgcctcctcc cccacctcct cgaagaagag
     4441 cagggcaccc gcgtagtcgt agtcccaggg agtcccgcag agagcggcga gcagggagag
     4501 gcagcctccg acgagagggc cctcggcacg gcccgggacg aggacgtccg ccgaggggac
     4561 ctcgagccgg tccggaccgc gtccctccag gacggccggg aaggaaggcg cggtccacgc
     4621 gtcgagaggc gcggagaggt cggccgcgac catcggcccg tggatcgaca ccaggcggca
     4681 gcgctcccgg aggaacgccg cgagcgcggt caggtcggag aagccgcagt gcagcttcgc
     4741 cgtatccgcg aggagacgct cggggagcct gtgcagcagg cgcgtcacgc cgtagccgcc
     4801 ccgcgagaag aggatcgcct cgacctccgg gtcttccacg agagcgacgt acccgctcac
     4861 gcgggcctcg tcgccgcccg cgagcccgag gagcggctcc gtctcgagaa cgttccgcgc
     4921 gacccggacc cggtagccga agcctcggag ggccgcgacg ccggcgtcca ggctctccgg
     4981 gcggaccggg ccggaaagcg ccgcgacgcc gacgaggctg ccgggacgga gccggcgcgg
     5041 acgccggaga ggggcgctca cccgatgcga ttccgcgggt cgctgaacca gcgcgcccag
     5101 tcggacgcgc tcccgcccgg gtccctcttc tcctcgtcgc gagcggcctt cgccgtcaga
     5161 cgccgcgcgc cggtctgccg gaacgcggcg accggaaggg tccgtccgcc cgccgcgtcg
     5221 accgactccg acaggacccg gtcgtccgtg acgaccgtca cgccgcgggg gtcgcgcgcc
     5281 tcgcgcaccc gccggacgat ctcctcgtcc gcgcttcgcg gagccgcgta gacgaccgtc
     5341 acgtcgccga gctgctggac cttcgggcga ccgtgctcgg gaggtccgtc gaagacgacg
     5401 gtcaggcgcc tccgcttcgc ccgggaaagc tccgccagct ccttcacgag cgtctcgcgc
     5461 gaaatgccct tctgcccgag gaggttgttg ccgtcgatca ggtcggccac gcctccattc
     5521 tgcgacatcg gcccggttcc ggcacgttcc ggagagagtc tgcgaccgcc gcggcgcgac
     5581 gcctcccgag gtgcgccggg aacgttcact tcggagaacg agacccgggc cggacgggct
     5641 ccgggggggc ttgccatccg ggcgcccccc cggccccccc ccaccttcaa tctgacgtaa
     5701 gtgacccggg ccggactggc tccggggggg cttgccatcc gggcgccccc ccg
//
LOCUS       DAOTEZ010000278         5726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_10068_length_5726_cov_4.866690, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000278 DAOTEZ010000000
VERSION     DAOTEZ010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5726
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10068_length_5726_cov_4.866690"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="P5164_13800"
     CDS             complement(<1..760)
                     /locus_tag="P5164_13800"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HRY45013.1"
                     /translation="MSPGWRTALVTTLRERCRVCFACVRECPAKAIRISGRQAEVIPE
                     RCIACGNCVRVCSQGAKQAVRSIAQVEELLASGAPVAACLAPSFPAEFPELPVPRLVG
                     AVRALGFSLVQEVAFGADLVARRYRELLAEPGRRSIATTCPAVVSYVERYHPRLVDLL
                     APVVSPMVATARVLKRLHGPDLHIVFVGPCIAKKGEDRWPGADPGVNEALTFGELREM
                     WLEKGIDPADASPSEFDPPRAGSGTIFPVSRGMLQ"
     gene            1082..1798
                     /locus_tag="P5164_13805"
     CDS             1082..1798
                     /locus_tag="P5164_13805"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DtxR family transcriptional regulator"
                     /protein_id="HRY45014.1"
                     /translation="MSEPLEDYLETIYLLLQEHGAARVRDIARARGVKAATVSIALGK
                     LGDAGLVDYARREHIRLTPTGEAAARRILTRHRLLTRFFEEVLGMSPHAASEQACSLE
                     HALTDEATDRLVRFFEFLGTRSATPAAFGQCPVGARPGDEHPCGAASPECARCQPSDA
                     KNAMSAATLKPGQSGIVTRITATGALRKRLLDMGILPETAIGLERSGPGGQPLWIRCQ
                     GARLALRRAEAGHILVRAAT"
     gene            2203..3330
                     /locus_tag="P5164_13810"
     CDS             2203..3330
                     /locus_tag="P5164_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45015.1"
                     /translation="MPLAGARLRSVVIGLAIAAAPLRAFGAEGAPETPPPAEAPETRQ
                     GGTGRELSAVLRELSLSGFFDVTARDRRSEENVFAMGDFELDLARELGSSVQVAAALV
                     VNDDGAALAVGFLDVHLLGGLVAPRGRLPVEKGLHVQLGRFDVPFGNDWQLYATKDRV
                     EASAPLTTEELLDGGVNDVGLRVLGSDGTFGYTAYALRGEGSGNAFGGRVSIAPLDVP
                     FRFPPRLGLVEVGVSLLHDADDDGKTERTALALDAQTRSGPLSVRGEYVRIDARPTEE
                     SPVRSVRDGWHVTAAFEAGDLPGGVSLTPYARYDTVRGDEGTAEGSGRTVRVTGGANA
                     ALFARLTLKAEYRRTLEAPEAIRAAEGFGRDAWLVQAVVTF"
     gene            3342..3803
                     /locus_tag="P5164_13815"
     CDS             3342..3803
                     /locus_tag="P5164_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45016.1"
                     /translation="MNAFPIARVPLLALALLAVPAVAAGAPAVRSAPSAHPTWGLDSC
                     LSCHAPAEREALGVRLSRPCAPLCTTCHDNRETHHPTGVRVPGLRAPLLLTGDGKLTC
                     CTCHDPAAPRRRDFAWTSQALVGRLVPRRGPHPTRLLAMRNDRGQLCRSCH"
     gene            complement(3853..4236)
                     /locus_tag="P5164_13820"
     CDS             complement(3853..4236)
                     /locus_tag="P5164_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005979942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HRY45017.1"
                     /translation="MKVVLRLRMSSHDAHYGGNLVDGARMLGLFGDVATELLIRLDGD
                     EGLFRAYDSVDFLAPVYAGDYIEAEGTISAVGNTSRKMAFEARKVIRSAPEVNDSAAE
                     VLEPAVVVCRATGTCVTPKEKQRRR"
     gene            complement(4310..4789)
                     /locus_tag="P5164_13825"
     CDS             complement(4310..4789)
                     /locus_tag="P5164_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45018.1"
                     /translation="MSRRSLFWLAVVAGVAFWLWKSGTVSKEDGTVRTSAAGTSVGAP
                     AAAGEACLFAAEEASRLTQEAADVLLRAPIDAAAFSTASQGAGSAISAAEGKCGGGSS
                     AAEQRAMEEVRGALSEMRGLLSDLSGALSGSGSASDAPRRRETIDQRLDAARAALRG"
     gene            5106..5438
                     /locus_tag="P5164_13830"
     CDS             5106..5438
                     /locus_tag="P5164_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45019.1"
                     /translation="MRTIRESGAVEAVIGERLDLVVTRPVGVPDRLRVEWPAAPAIEG
                     AAVRFVRRRVEAPPPENDGGVTTFHYELEAARPGAARVTLTPKPASRDAAQPPVVLEV
                     AVRASPSR"
     gene            complement(5445..>5726)
                     /locus_tag="P5164_13835"
     CDS             complement(5445..>5726)
                     /locus_tag="P5164_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45020.1"
                     /translation="PGSLAPVRATVFAAAAVGLAALRARTGAAEAGGLAWCVLVAAAA
                     RVVLQDLPDGRPLPLFAGFVACGLGLLVVGRLRSKPTAVAGPSEPSGEG"
BASE COUNT          722 a         2248 c         2023 g          733 t
ORIGIN      
        1 cctggagcat cccgcggctc accgggaaga tcgtgccgct gcccgcccgc ggcgggtcga
       61 actccgaggg cgaagcgtcg gcggggtcga tgcctttctc gagccacatc tcgcggagct
      121 ccccgaacgt cagggcctcg ttgacgccgg ggtccgcgcc gggccagcgg tcctccccct
      181 tcttcgcgat gcagggtccg acgaagacga tgtggaggtc cggcccgtgg aggcgcttca
      241 ggacccgcgc cgtcgcgacc atcggcgaga cgacgggcgc cagcaggtcc acgaggcgcg
      301 ggtggtagcg ctccacgtag ctgacgaccg cgggacaggt cgtcgcgatg gagcgccggc
      361 cgggctcggc gaggagctcg cggtagcggc gggcgacgag gtcggcgccg aacgcgacct
      421 cctggacgag cgagaagccg agcgcgcgga ccgcaccgac gagccggggg accggaagct
      481 cggggaactc ggccgggaag ctgggcgcga ggcaggccgc gaccggggcg cccgaggcga
      541 ggagctcctc gacctgggcg atcgagcgga cggcctgctt ggccccctgg ctgcagaccc
      601 tcacgcagtt gccgcaggcg atgcagcgct ccgggatcac ctccgcctgc cgcccggaaa
      661 tccggatcgc cttggccggg cactcgcgaa cgcaggcgaa gcagacgcgg cagcgctccc
      721 ggagcgtcgt gacgagtgct gtccgccagc cggggctcac gcgcgcgcct tccggaagac
      781 ttcggcttcg gggcgggtcg cctccgcgca gcccgccgcc ccgtcgatcc ggacccggct
      841 ggaggtcctt cgcaaccgtc gcctccccgc ggcttgccgg gcagcggccc gccgcaacga
      901 atcatcatag accttcggaa gcgcgcctcg ccgcaggggg aacgcgaccg acgcggcgcc
      961 cgatcgctct cgccggagct ggcccggtcg gcgcccgccc gccgccgtcc ggcccttgac
     1021 gctcccggac aattgttaga tgcttctaac ggtgagtgcc gccgccgtcg caccgcagcc
     1081 gatgagcgag cccctcgagg actacctcga gacgatctac ctgctcctgc aggagcacgg
     1141 agccgcacgg gtccgcgaca tcgcccgggc gcgcggcgtg aaggccgcga cggtctcgat
     1201 cgccctcggg aagctcgggg acgccggcct cgtcgactac gcgcgccggg agcacatccg
     1261 tctcaccccg acgggggaag cggcggcgcg ccgcatcctg acccgccacc gcctcctgac
     1321 tcgcttcttc gaagaggtcc tcggcatgtc gccgcacgcg gcgagcgagc aggcctgctc
     1381 gctcgagcac gcgctcaccg acgaggcgac ggatcgcctc gtgcgcttct tcgagttcct
     1441 cggcacccgc tccgccactc ccgccgcctt cggacagtgc cccgtcgggg cgcgccccgg
     1501 agacgagcat ccctgcggcg ccgcctcgcc ggagtgcgcc cgctgccaac cgtccgacgc
     1561 gaagaacgcg atgagcgccg ccaccctgaa gcccggacag agcggcatcg tcacccggat
     1621 caccgcgacg ggcgccctcc gcaagcgcct cctcgacatg ggcattctcc cggagacggc
     1681 gatcggcctg gagcgctccg gacccggcgg ccagccgctc tggatccgct gccagggggc
     1741 gcgtctcgcg ctgcgccgcg cggaggccgg tcacatcctc gtacgggcgg ccacgtgatc
     1801 gccctccctt tttttggccg gagagttaga tgcctctaac acacgtcccg agccccgtcc
     1861 ctcccgagcc gcttgcgcgg cagcccgagc cccgacacgt cggtgcgggc gggctcgcct
     1921 gcacctcgcg cgggccgcgg ctctcctgaa ggaccccgtc gttggatacg gtcctcgcga
     1981 cgctcatcgt cctcgccgct gccgccggga tcgcccgctc ggcctaccgg cgcctcgccc
     2041 gtcgtaccga ggcctcctgc tcctccggtt gcgaaggctg cgcgctcgcg tcgcgttgcg
     2101 gcgccgcttc cgcgggcggg gcatcgccga cggcgggagc acccctcccg ccgcagcccg
     2161 gcggcagcac ggtctcgcct cggcggatcg gagagcgctg agatgccgct tgccggcgcc
     2221 cggcttcggt ccgtcgttat cgggctggcc atcgcggccg cgcctctccg cgccttcggc
     2281 gccgagggcg ctccggagac cccgcctccc gcggaggcgc ccgagacccg ccaggggggg
     2341 accggccgag agctctcggc ggtcctccgg gagctctccc tctccggctt cttcgacgtc
     2401 accgcacggg accgccggag cgaggagaac gtcttcgcga tgggcgactt cgagctggac
     2461 ctcgcccggg agctcggcag cagcgtccag gtcgcggcgg ccctcgtcgt aaacgacgac
     2521 ggggcggcgc tcgcggtcgg cttcctcgac gtccacctcc tcggcggcct cgtcgcgccg
     2581 cgcggccggc tccccgtcga gaaggggctc cacgtccagc tcggccgctt cgacgtcccg
     2641 ttcgggaacg actggcagct gtacgcgacg aaggaccggg tcgaggcgag cgcgccgctg
     2701 acgaccgagg agctcctgga cggcggcgtg aacgacgtcg gactgcgcgt cctcggcagc
     2761 gacggcacgt tcggctacac ggcctacgcc ctgcgcggag aaggctccgg gaacgccttc
     2821 ggcggtcggg tgagcatcgc ccctctcgac gtgccgttcc gcttcccccc gcggctcggc
     2881 ctcgtcgagg tcggcgtctc gctgctccac gacgcggacg acgacgggaa gacggagagg
     2941 acggccctcg ccctcgacgc gcagacgcgc tcgggcccgc tctcggtccg gggcgagtac
     3001 gtccgcattg acgcccggcc gaccgaggag agccccgtcc gttcggtccg ggacgggtgg
     3061 cacgtcacgg cggcgttcga ggcgggcgac cttcccggcg gcgtctccct caccccgtac
     3121 gcgcgctacg acaccgtccg cggcgacgag gggacggcgg aggggtcggg ccgaacggta
     3181 cgcgtcaccg gcggcgcgaa tgccgctctc ttcgctcgcc tcacgctcaa ggccgagtac
     3241 cgacgcactc ttgaggctcc ggaggccatc cgagccgcgg agggcttcgg cagggacgcc
     3301 tggctcgtcc aggccgtcgt cacgttctga gggagagaag catgaacgcc ttccccatcg
     3361 cccgggtccc gctcctcgcg ctggcgctcc tcgccgtccc ggccgtggcg gcgggtgcgc
     3421 ccgcggtccg ctccgctccc tcggcgcacc cgacctgggg gctcgactcc tgcctctcct
     3481 gtcacgctcc cgccgagagg gaagcgctcg gcgtccgcct ctcccgcccc tgcgccccgc
     3541 tctgcacgac gtgccacgac aaccgcgaga cgcatcatcc gacgggagtc cgcgtgccgg
     3601 gcctccgcgc gccgctcctc ctgacgggcg acggaaagct gacttgctgc acctgtcacg
     3661 acccggcggc gccgcggcgg cgagacttcg cctggacgtc gcaggcgctc gtcggccggc
     3721 tcgtcccgcg gagggggccg cacccgacga ggctcctggc gatgaggaac gaccggggcc
     3781 agctctgccg gagctgccac tgacggagcg gcgcgatccg gggcgcggac gcgccccgga
     3841 tcgccgaagc cgctaccggc ggcgctgctt ctccttcggg gtgacgcagg tgcccgtcgc
     3901 gcggcagacg acgacggcgg gctcgaggac ctcggccgcg gagtcgttca cctcgggcgc
     3961 cgagcggatc accttgcgcg cctcgaaggc catcttccgg ctcgtgttcc cgacggcgga
     4021 gatcgtcccc tccgcctcga tgtagtcccc cgcgtagacc ggcgcgagga agtcgacgga
     4081 gtcgtaggcc cggaacagcc cctcgtcgcc gtcgagccgg atcagaagct ctgtcgcgac
     4141 gtcaccgaac agcccgagca tccgggcccc gtcgacgagg ttgccgccgt agtgggcgtc
     4201 gtggctcgac atccggaggc ggaggacgac cttcaggcct tcgccgggct tcttctcttc
     4261 gctcaactcg tgcacctcgt tcgcttactc cggcccggcc cccggcggct cagccccgga
     4321 gggccgcgcg cgcggcgtcg agccgctggt cgatcgtctc acgccttcgg ggcgcgtccg
     4381 aggcgctccc gctgccggag agcgcgcccg agaggtccga gagcaggccg cgcatctccg
     4441 agagcgcgcc ccgaacctcc tccatcgccc gctgctcggc cgccgaggag cctcccccgc
     4501 acttcccttc ggccgcggag atcgccgagc cggcgccctg cgacgccgtc gagaacgccg
     4561 cggcgtcgat cggcgcgcga aggaggacgt ccgcggcctc ctgcgtgagg cggctcgcct
     4621 cctcggcggc gaagaggcag gcctctcccg cggccgccgg tgcgccgacg ctcgtgccgg
     4681 ccgcggaggt acggaccgtc ccgtcctcct tcgagaccgt ccccgacttc cagagccaga
     4741 acgcgacgcc cgcgacgacg gcgagccaga acagcgaacg acggctcatg acgacctcct
     4801 tcttccgacc tcggattcga cgggcacgac ccgggcccga ctctatcgcc cgaccggggc
     4861 gggcgcagga ggaaaggccc cgctggagtc tgaggcgggg cacgcgggca cggcgccccc
     4921 ctccgctccc ggaccggcgc ggtgatagcg ttcccgccgt ggcgcttcga gctcggcctt
     4981 cccggctccc ggcggcgttc ctcgctctcg cgacgagctt cggctgctcc gatcccgccc
     5041 ccgctgccgc ccccgcgccc ccttccgttc cggccgcccg gagggccgtc ccaggagcgc
     5101 tcgagatgag gaccatccga gaatccggcg ccgtcgaagc cgtcatcggc gagcgcctcg
     5161 acctcgtcgt cacccgcccc gtcggcgtcc ccgatcggct ccgggtcgaa tggcccgccg
     5221 ctccggcgat cgaaggggcc gcggtccggt tcgtgaggag gcgcgtcgag gcgcccccgc
     5281 ccgagaacga cggcggcgtg acgacgttcc actacgagct cgaggcggcg cgccccggag
     5341 ccgcccgcgt cacgctcacg ccgaagcccg cgagccgcga cgccgcgcag ccgcccgtcg
     5401 tgctcgaggt ggcggtccgg gcctccccgt cgcgctgagg cgcgtcaccc ctccccggac
     5461 ggttccgatg gacccgccac cgcggtcggc ttcgagcgga gccgtccgac gacgagcagc
     5521 ccgaggccgc aagcgacgaa tccggcgaag agcggcagcg gcctgccgtc cggcaggtcc
     5581 tgcagcacga cgcgcgcggc cgcggcgacg aggacgcacc acgcgaggcc gccggcctcc
     5641 gcggcgcccg tccgggcccg cagcgcggcg aggccgacgg ccgcggcggc gaagaccgtc
     5701 gcgcggacgg gcgcgagcga tcccgg
//
LOCUS       DAOTEZ010000279         5723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_10076_length_5723_cov_4.728652, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000279 DAOTEZ010000000
VERSION     DAOTEZ010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10076_length_5723_cov_4.728652"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..4331
                     /locus_tag="P5164_13840"
     CDS             234..4331
                     /locus_tag="P5164_13840"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRY45021.1"
                     /translation="MNRPARRVALVSFVALAVAISVCSAPPAEAVSTSIVISQVYGGA
                     GCGTAGCSTYKNDYVELFNLGSTPVSLNGMSVQYASAAGSFNAATALPDVTLQPGQYF
                     LVAQAFSNNGVTALPAPDATGSIAMSATAGKVLLANTATALGCGATGASPAPCSTSQQ
                     ALIVDLVGFGTTATLFEGTGPAPAPSTTTADLRKTNGCTETDQNAADFAAGAPTPRNT
                     ASPLSACAVPDTAPSVVSTNPTAGATAVPVASDVSVTFTEPVTVSAASFTLECPSGTP
                     FAGGFAVSGSGTATITINPTGDLPAGTACNVGIVAANVTDLDGTPDPMASPYAWTFTT
                     ASATPALSINDVTLAEGNSGTTTFQFTVSLSVPAGPGGVTFDIATLDGTATTADNDYV
                     ASSLTGQTIPAGSSTYTFNVTVNGDTTVEANETFFVNVSNATGATVADGQGQGTITND
                     DVALTPIHDIQGNGLLSPLDGTTVTTRGIVTARKFNNGFFIQEPDASVDADPATSEGV
                     FVYTNAAPTVAVGDLVQVAGTVDEYVQSGNFGSITELVGPLTITVVSSGNPLPAATAL
                     TTTMPSPAGSWYQLEHLEGMRVSVPGFLVTAATDGNPGGNYTTGTSYGDCYGVVTGLA
                     RPLREDGIAYPNNPPAGTSIPPLLRWDGNPEQLRVDTDGQVSATRINLSARTVLPAFD
                     GVLDYAFSRYSILPDVGSLPASPAEFPPTAVTAPLASEFTVASYNLQRFFDDVNDPAI
                     SEPVLTPTQYEARLAKASLHVRNFLLMPDVVGVVEVENLSTLQTLATRISTDAVAASQ
                     PDPLYQAYLIEGNDVGGIDVGFLVKTSLVGANPRVQVLTVTQELDGTLFTNPDSSTET
                     LNDRPPLMLRAIVHGASGGTYPVTVLVNHLRSMNDVDSDAAGSYGWPTLGARVRAKRQ
                     AQAVDLANLVQTLQGENVILVGDFNAFDVNDSLGHLMATIVGAPADDATTAVSGDGAD
                     LVNPDLVDLGAASPPAERYSYVFGFTAQKLDHALVNAALVSSSLARRVEYARINSDFP
                     EIDRSTPTARLSDHDPLVVYVQAAALASAGVSVTMTDAPDPVFPGAGLTYAITVTNAG
                     PDPATTLVLTNALPAGTVFASLSSPAGWSCTTPAVGASGTVTCSAATLAPAANAAFTL
                     EVTVDAATPGGTVLSSTASVAASSGDPSLSDNSATTTTTVLTPPAVFATKTVLGVYRP
                     GGSVTYTIVLRNNGSTAQADNPGDELTDVLPSSLALVSASATSGTAVATVATNTVTWN
                     GSIPAGGSVTVTIVATISPSVTVGTSISNQATAAYDADGNGTNEASAPSDNPATGAAG
                     DATVFTVNRDAAGIVAVPTLDGVGLGILSLLVALGGALVLGRRLS"
     gene            complement(4408..>5723)
                     /locus_tag="P5164_13845"
     CDS             complement(4408..>5723)
                     /locus_tag="P5164_13845"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRY45022.1"
                     /translation="REGGVNAAWRRGTIGVTAGFRRTTAGGDLPNEFFAGTDLSAALD
                     VRLSDGARTGVTIRRETSRTGIPFEGATTTPRRFTTADTTLVSVPLSVALGAGTRLEA
                     AGTFATDEPTFSDPDEPWGFTFSETRARRAGGRLVLSHESSANRISVGADLERTKVDN
                     EDAYGVQLDGLTTRTWSVFAEDRLSLARDRFIVTAGVRRDEHDAFGASTNPRVALSWK
                     ASSTLRLRAAAGSAFRAPATGELYYPFSGNPELEPERSVSYEAGAEWTLGGGASLEAT
                     VFRSDVKDLIRYDFAFQRNVNVGRARMRGVEARLFMPLTRTAWARAGWTRLDTEDRDT
                     GLPLLRRPKQRASLTVGEDLGRGASAEVTGLWVGERDDVDATTYLRVTSPAYFRLDAA
                     LTLPPVAWGLSPFLRVTNLLGRDYAEVAGYPSPGRRFVAGVDVAF"
BASE COUNT          853 a         2225 c         1859 g          786 t
ORIGIN      
        1 agcgagatcg taagcgaggc gagcatagtg gcggccctcc gccgccccgc cggtcctcga
       61 ggtccgcgct gcgtccgccg cgagtccact tcatggtccg tgcctcctct cgcccacaga
      121 ggggttcacg aaagattagc ggacattccc gcccccgcgt ggataagatc ccagaccgct
      181 ttccgcgcga gcccgccccc gagcgcgcat ttcactcccg aagggagatc ccgatgaacc
      241 gtccggcccg gagagtcgcc ctcgtttcgt tcgtggccct cgcggtcgca atcagtgtgt
      301 gctcggcccc tcctgcggag gcggtttcga cgagcatcgt catcagccag gtctacggcg
      361 gcgcgggctg cggcacggcc ggatgctcga cctacaagaa cgactacgtc gagctcttca
      421 acctcgggtc gacgcctgtc agcttgaacg ggatgtccgt gcagtacgcc tcggcggcag
      481 ggagcttcaa cgccgcgacc gcgctcccgg acgtgaccct gcagcccggc cagtacttcc
      541 tggtcgcgca agccttcagc aacaacggcg tgaccgcgct cccggctccc gacgcgacgg
      601 ggagcatcgc gatgagcgcc acggccggga aggtcctcct cgcgaatacg gcgaccgcgc
      661 tcggctgcgg agccacaggc gcctccccgg cgccctgctc cacttcccag caggcactca
      721 tcgtcgacct cgtagggttc ggcacgacgg ccacgctgtt cgaggggacg gggcccgcac
      781 cggcgccaag caccacgacc gccgacctga ggaagacgaa cggatgtacc gaaacggacc
      841 agaacgcggc ggacttcgcg gcaggcgcac ccacgccccg taacaccgct tctccgctga
      901 gcgcttgcgc cgttcccgac accgcaccct ccgtcgtctc cacgaacccc accgcgggtg
      961 caacggccgt cccggtcgcc tcggacgtca gcgtcacctt cacggaaccc gtgacggtct
     1021 ccgcggcctc gttcacactc gagtgcccct ccgggacccc cttcgcggga ggtttcgccg
     1081 tctcgggaag cggcacggcg accatcacga tcaaccccac gggcgacctg ccggccggca
     1141 cggcctgcaa cgtcgggatc gtcgccgcga acgtcacgga cctcgacggg acgccggacc
     1201 cgatggcctc gccctacgcg tggacgttca cgacggcctc cgccaccccg gccctctcca
     1261 tcaacgacgt caccctcgcc gaggggaact ccgggacgac gacgttccag ttcacggtct
     1321 ctctcagcgt gcccgccggg ccgggcggcg tcacgttcga catcgcgacc ctcgacggca
     1381 ccgccacgac ggccgacaac gactacgtcg cgagctcgct caccgggcag acgatccccg
     1441 ccgggagctc gacctacacc ttcaacgtga cggtgaacgg cgacacgacc gtcgaggcga
     1501 acgagacctt cttcgtgaac gtctcgaacg ctacgggcgc gacggtcgcc gacgggcagg
     1561 gacagggcac gatcacgaac gacgacgtcg cgttgacgcc gatccacgac atccagggga
     1621 acggcctcct ctcgcccctc gacggaacca ccgtcacgac ccggggcatc gtcaccgcgc
     1681 gcaagttcaa caacgggttc ttcatccagg agcccgacgc ctccgtcgac gccgacccgg
     1741 cgacctccga gggggtcttc gtctacacca acgccgcacc caccgtggcc gtcggcgacc
     1801 tcgttcaggt ggccggcacc gtcgacgagt acgtccagag cggcaacttc gggtcgatca
     1861 ccgagctcgt cggcccgctc acgatcacgg tcgtctccag cgggaacccg ctcccggcgg
     1921 cgacggcgct cacgacgacg atgccctcgc cggcggggag ctggtaccag ctcgagcacc
     1981 tcgaggggat gcgggtcagc gtgcccggct tcctcgtcac ggccgcgacg gacgggaacc
     2041 ccggcggcaa ctacacgacg gggacctcct acggcgactg ctacggcgtc gtcaccggcc
     2101 tcgcccggcc gctccgcgag gacgggatcg cgtacccgaa caacccgccc gccggcacca
     2161 gcatcccgcc gctcctccgc tgggacggga accccgagca gctccgcgtc gacaccgacg
     2221 gccaggtctc ggcgacccga atcaacctct cggcccggac ggtcctcccg gccttcgacg
     2281 gcgttctcga ctacgctttc tcccggtact cgatcctccc cgacgtcggc agcctcccgg
     2341 ccagccccgc cgagttcccc ccgacggccg tcacggcgcc ccttgcgagc gagttcacgg
     2401 tcgcctcgta caacctccag cgcttcttcg acgacgtgaa cgacccggcg atcagcgagc
     2461 cggtcctgac gccgacccag tacgaggcgc gcctcgcgaa ggcctccctg cacgtccgga
     2521 acttcctgct catgcccgac gtcgtcggcg tcgtcgaggt cgagaacctc tcgacgctcc
     2581 agacgctcgc gacgcgcatc agcacggacg cggtggcggc gtcgcagccc gacccgctct
     2641 accaggcgta tctcatcgag gggaacgacg tcggcgggat cgacgtcggg ttcctcgtga
     2701 agacctctct cgtcggcgcg aacccgcgcg tccaggtcct caccgtgacg caggagctgg
     2761 acgggacgct cttcacgaac ccggacagct cgaccgagac cctcaacgac cggccaccgc
     2821 tcatgctccg ggccatcgtt cacggcgcca gcggcggaac gtacccggtg accgtcctcg
     2881 tgaaccacct ccggtcgatg aacgacgtcg acagcgacgc cgccggttcg tacggctggc
     2941 cgaccctcgg cgcacgcgtc cgcgccaagc ggcaggcgca ggccgtcgac ctcgcgaacc
     3001 tcgtccagac cctgcagggc gagaacgtca tcctcgtcgg cgacttcaac gcgttcgacg
     3061 tgaacgacag cctcggccac ctgatggcga cgatcgtcgg cgcccccgcc gacgacgcga
     3121 ccacggcggt ctctggcgac ggcgcggacc tcgtgaaccc ggacctcgtc gacctcggcg
     3181 cggcttcgcc gcccgccgag cgctactcct acgtcttcgg cttcacggcc cagaagctcg
     3241 accacgcgct cgtcaacgcc gcgctcgtga gctcgagcct cgcccgtcgc gtcgagtacg
     3301 cgcgcatcaa ctccgacttc cccgagatcg accgatcgac gccgacggcc cgcctctccg
     3361 accacgaccc cctcgtcgtc tacgtccagg ccgccgccct cgcgtccgcc ggcgtctcgg
     3421 tcaccatgac cgacgctccg gacccggtct tccccggcgc cggcctgacg tacgcgatca
     3481 cggtcacgaa cgccggaccg gacccggcca cgacgctcgt cctgacgaac gcccttcccg
     3541 cgggcaccgt cttcgcctcc ctgtccagcc ccgccggctg gagctgcacg acccccgcgg
     3601 tcggcgcgtc gggcaccgtg acgtgctccg ccgcgacgct cgcgcctgcc gcgaacgcgg
     3661 ccttcactct cgaggtcacc gtcgacgccg ccactcccgg cggcacggtc ctctcgagca
     3721 ccgcgagcgt cgcggcctcc tccggcgacc cgagcctctc cgacaactcg gcgaccacca
     3781 ccacgaccgt actgacgccg cccgccgtct tcgcgacgaa gaccgtcctc ggcgtctacc
     3841 ggcccggcgg gtcggtgacg tacacgatcg tccttcggaa caacggctcg accgcccagg
     3901 ccgacaaccc gggtgacgag ctgaccgacg tcctcccttc gagccttgcc ctagtctccg
     3961 cttccgcgac ctccggcacg gccgtcgcga cggtcgcgac gaacaccgtg acctggaacg
     4021 gctcgatccc ggcgggcggc tccgtcacgg tcacgatcgt cgcgacgatc tcgccttcgg
     4081 ttaccgtcgg cacgtcgatc tcgaaccagg cgaccgccgc gtatgacgcc gacgggaacg
     4141 gcacgaacga ggcctcggcc ccgagcgaca acccggcgac gggcgccgcg ggcgacgcga
     4201 ccgtcttcac cgtgaaccgc gacgcggctg gcatcgtcgc cgttccgact ctcgacggcg
     4261 tcggcctcgg gatcctgtcg ctcctcgtcg cgctcggcgg cgcgctcgtc ctcgggcggc
     4321 gcctctcgta gcgccggtcg ccgatcctcc ggtctacgaa gggccgggag cctcgcggct
     4381 cccggccctt ctcctatgcg tggagcgtca gaaggcgacg tcgacgccgg cgacgaagcg
     4441 gcgtccgggc gacgggtagc ccgcgacctc ggcgtagtcg cggccgagga ggttcgtgac
     4501 gcggaggaac ggggagaggc cccaggcgac gggcggaagc gtgagcgcgg cgtcgagccg
     4561 gaagtaggcg gggctcgtga cgcgcaggta cgtcgtcgcg tcgacgtcgt cccgctcacc
     4621 gacccagagc ccggtcacct cggcgctcgc cccgcgcccg aggtcctcgc cgacggtcag
     4681 gctggcccgc tgcttcggcc gtcggaggag aggaagaccg gtgtcgcggt cctccgtgtc
     4741 gagccgggtc cagccggcgc gcgcccaggc cgtcctcgtc agcggcatga agagcctggc
     4801 ctcgacgcct ctcatccggg ctcggccgac gttgacgttc ctctggaagg cgaagtcgta
     4861 ccggatcagg tccttcacgt cgctccggaa gacggtcgcc tcgagggagg cgccgccgcc
     4921 gagggtccac tcggctcccg cctcgtacga gacggagcgc tccggctcga gctccgggtt
     4981 cccggagaac gggtagtaga gctcgcccgt cgccggggcc cggaacgccg agcccgccgc
     5041 ggcccggagc cgaagcgtgg aggaggcctt ccaggaaaga gcgacgcgcg ggttcgtcga
     5101 tgcgccgaag gcgtcgtgct cgtccctgcg gactccggcc gtgacgatga agcggtcccg
     5161 ggcgagggag aggcggtcct cggcgaagac cgaccaggtg cgcgtcgtca ggccgtcgag
     5221 ctggacgccg tacgcgtcct cgttgtccac cttcgtccgc tcgaggtcgg cgccgacgga
     5281 gatccggttc gcggagctct cgtgcgagag gacgaggcgc ccgccggccc gtcgggcgcg
     5341 cgtctcggag aacgtgaagc cccagggctc gtccgggtcg gagaacgtcg gctcgtcggt
     5401 cgcgaaggtg ccggccgctt cgagcctcgt ccccgcgccg agcgcgacgg agagcgggac
     5461 ggagacgagc gtcgtgtcgg ccgtggtgaa gcggcgcggc gtcgtcgtcg ccccctcgaa
     5521 ggggatcccc gtccgagacg tctcgcggcg gatggtgacg cccgtccgcg ctccgtcgga
     5581 cagccggaca tcgagcgcgg cggagaggtc ggtcccggcg aagaactcgt tcgggaggtc
     5641 gccgccggcg gtggtccgcc gaaaccccgc ggtcacgccg atcgtgccgc gccgccaggc
     5701 ggcgttcacg cccccctcgc gtg
//
LOCUS       DAOTEZ010000280         5721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_10080_length_5721_cov_5.324921, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000280 DAOTEZ010000000
VERSION     DAOTEZ010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10080_length_5721_cov_5.324921"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="P5164_13850"
     CDS             <1..424
                     /locus_tag="P5164_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRY45023.1"
                     /translation="PGADPAAVVFDACAAAKARGADLLLVDTAGRLHTKHNLMEELSK
                     IRRIAGREIPGAPHEVLLVLDAVTGMNGLAQAREFLKAAGVTGLVLTKMDGTAKGGVI
                     LAIVRELNLPVRYVGVGETADDLLDFDPAGFAAALIDD"
     gene            417..1535
                     /gene="ribD"
                     /locus_tag="P5164_13855"
     CDS             417..1535
                     /gene="ribD"
                     /locus_tag="P5164_13855"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRY45024.1"
                     /translation="MTDLASAFERVFELAERGRYSTSPNPRVGAVVVDAAGAVVGEGW
                     HERAGSPHAEAVALAEAGSRARGATVVVNLEPCAHHGRTPPCADALVAAGVSRVVFSL
                     LDPDPRTAGKGRERLRAGGVATETGAFAERAERLNEPFLTAARSGRPFVHLKWGASLD
                     GKIATVSGRSRWVTCEEARADGMRLREECDAILVGAGTVLADDPALTRRAGLNRSVVP
                     HRRVVVDGRLRVTGDRNVFSPRAPGEAWLATAVPEGDPRLAPFRDAGVRVLSLPSTTP
                     GRVDLAALLARLGGEEARSLLLEGGGETSFSFLESGLVDRVTAYYAPLLLGGRETPSA
                     LAGAGFPSLSSAPRLSHLEVEPLGDGFRVTGRVGRPAA"
     gene            1545..2186
                     /locus_tag="P5164_13860"
     CDS             1545..2186
                     /locus_tag="P5164_13860"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008406352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRY45025.1"
                     /translation="MFTGLVEAAAKVRAVGRRAGGALLDVERPEAFVDVARGESISVS
                     GVCLTIVPGAEAGLLRFDVSAETLERSTLGALRPGDAVNLERALAAGGRMGGHVVQGH
                     VDATTPLTRLERSGDFWTLGVRLDPSFSRYVVEKGSIALDGVSLTVASLAEEELTVAV
                     IPETFRATTLGRRRPGDLVNVEVDVLAKYVERLLRRAVPATSADERLRALLGS"
     gene            2183..3307
                     /gene="hemW"
                     /locus_tag="P5164_13865"
     CDS             2183..3307
                     /gene="hemW"
                     /locus_tag="P5164_13865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRY45026.1"
                     /translation="MTTRPGAYVHVPYCAHRCSYCSFVAVTGRETEREYFDAVVAEIG
                     ARAGEVPGPLETVYLGGGTPSFADPGSLARVLGALERTWGLVADAEVTAEANPDDLVP
                     ERLDALVRLGVNRLSVGVQSLRDAELAPLERRHDAASARDAVRLALNRGLRVSADLMI
                     GIPGQTRESLLEGLSELLSSGVGHVSVYLLEIEKAPRLVALRDSAPSLFAADDEMAER
                     WERVDDACRAAGLPRYETSNWARPGEESRHNLKYWRREPVVAFGVSAASFDGLVRRTN
                     GGSIPAYVAAVRATGTAFVAEERLPSEAALREEVLLGLRTVVGVAAGRFTEAVDTLPP
                     ADRARLSDAAAAGLLETESGRVRLTRAGVLLSNEVFALLL"
     gene            3442..5661
                     /locus_tag="P5164_13870"
     CDS             3442..5661
                     /locus_tag="P5164_13870"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017841210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRY45027.1"
                     /translation="MNAETLVYVIPVFGLAGLLYTWLKSSWVARQDVGTERMAKIAGA
                     IQQGAMAFLRAEYRVLGVFVAVVALLLGISGALQEGSSPLIAAAFVAGALCSALAGLI
                     GMRVATKANVRTTQAARRGLGPALEIAFAGGSVMGLGVVGLGVLGLGGCFLVFSKLFA
                     GDVHKTIAVITGFSFGASSIALFARVGGGIYTKAADVGADLVGKVEAGIPEDHPLNPA
                     TIADNVGDNVGDVAGMGADLFESYVGSIVGAMVLGAAFMTGRLVTGEPFTADGFQGLS
                     AVLLPLVLAGVGILTSIAGTFFVRVREGGSPQRALNTGEFGSSIVMVVLSYFIIRGML
                     PGSFVFDDVLYGAERTITSLGVFFSTVIGLAAGLAIGVLTEHYTGTGTGPVTSIARQS
                     VTGSATNIIAGLGVGMRSTAWPVLILAASIIGAFHFGGLYGIAIAAVGMLSNTGIQLA
                     VDAYGPISDNAGGIAEMAELPKDVRSRTDKLDAVGNTTAAIGKGFAIGSAALTALALF
                     GAFMTAAHLKTIDVSKAEVMAGLLVGAMLPFLFSSMAMAAVGRAAMSMIQEVRRQFSE
                     IPALKAAIFAMDAHSGKDFKDWPVEDQKIFEDALGKAEYGKCVEISTTAAIKEMVAPG
                     LLAVIVPVVVGFGPRLLGLGSGAEMLGGLLAGVTATGVLLAIFQSNSGGAWDNAKKMF
                     EEGVEIDGHRYFKGSDPHKAAVVGDTVGDPFKDTSGPSLNILLKLMSVIALVIATLL"
BASE COUNT          707 a         2064 c         2089 g          861 t
ORIGIN      
        1 cccaggcgct gaccccgccg ccgtcgtctt cgacgcctgc gccgcggcga aggcgcgcgg
       61 ggccgacctc ctcctcgtcg acacggcggg gcgccttcac acgaagcaca acctgatgga
      121 ggagctctcc aagatccggc ggatcgccgg gcgcgagatt cccggcgcgc cgcacgaggt
      181 cctcctcgtc ctcgacgcgg tgaccgggat gaacggcctc gcccaggccc gcgagttcct
      241 gaaggccgcg ggcgtcaccg gcctcgtcct gaccaagatg gacgggaccg cgaagggggg
      301 cgtcatcctc gccatcgtcc gcgagctgaa cctcccggtg cggtacgtcg gcgtcggcga
      361 gacggccgac gacctcctcg acttcgaccc ggccggcttc gccgccgcgc tgatcgatga
      421 ctgacctggc ctccgccttc gagagggtct tcgagctcgc cgagcggggg cgctactcca
      481 cctcgccgaa cccgcgcgtc ggcgcggtcg tcgtcgacgc ggccggagcc gtggtcggcg
      541 agggatggca cgagcgggcc ggaagcccgc acgcggaggc ggtcgctctc gccgaagcgg
      601 ggagccgcgc gcgcggggcg accgtcgtcg tgaatctcga gccgtgcgcc caccacgggc
      661 ggacgccgcc ctgcgcggac gccctcgtcg cggccggcgt ctcccgggtc gtcttttccc
      721 tcctcgaccc ggacccgcgg acggcgggga aggggcgcga gcggctccgc gccggcggcg
      781 tcgcgaccga gacgggcgcc ttcgccgagc gcgccgagcg tctcaacgag ccgttcctga
      841 cggccgcgcg gagcggccgg ccgttcgtcc acctgaagtg gggggcgtcg ctcgacggga
      901 aaatcgcgac ggtctccggg agaagccgct gggtgacgtg cgaggaggcg cgggccgacg
      961 ggatgcgcct gcgggaggag tgcgacgcga tcctcgtcgg cgcgggaacg gtgctcgccg
     1021 acgatcccgc cctgacgcgc cgggcaggcc tgaaccgctc ggtcgtgccg caccgccgcg
     1081 tcgtcgtcga cgggcgcctc cgggtcacgg gcgaccggaa cgtcttctcg ccccgcgcgc
     1141 cgggcgaggc ctggctggcc acggccgtgc ccgagggcga cccgcgcctc gcgccgttcc
     1201 gtgacgccgg agtccgggtc ctgtcgcttc cgtcgacgac tcccggccgg gtcgacctcg
     1261 ccgcgctcct cgcccggctc ggcggcgagg aggcgcgctc gctcctcctc gaggggggcg
     1321 gcgagacgtc cttctccttc ctcgagtcgg gcctcgtcga ccgcgtcacg gcctattacg
     1381 cgccgctcct cctcggcggg cgggaaacgc cctcggccct cgcgggcgcc gggttcccga
     1441 gcctttcgag cgctccgcgg ctctcgcacc tcgaggtcga gccgctcggc gacggcttcc
     1501 gcgtgaccgg gcgcgtcggc cgtccggcgg cgtaggatcg gctcgtgttc acgggactcg
     1561 tcgaagctgc cgcgaaggtc cgagccgtcg ggaggagagc gggcggcgcc ctgctggacg
     1621 tcgaacggcc ggaggcgttc gtcgacgtcg cccgcgggga gagcatctcc gtctccggcg
     1681 tctgcctcac gatcgtcccc ggcgccgagg ccggtcttct caggttcgac gtctccgccg
     1741 agacgctcga gcggtcgacg ctcggcgcgc tccgaccggg cgacgccgtg aacctcgagc
     1801 gcgccctcgc cgccggcggc cggatgggcg ggcacgtcgt gcaggggcac gtcgacgcga
     1861 cgacaccctt gacgaggctc gagaggagcg gcgacttctg gacgctcggc gtccggctcg
     1921 acccctcgtt ctcgcggtac gtggtcgaga agggctcgat cgcgctcgac ggggtttcgc
     1981 tgacggtcgc ctccctcgcg gaggaggagc tgacggtcgc ggtcatcccg gagacgttcc
     2041 gcgcgacgac gctcggccga cgccgcccgg gcgatctggt gaacgtggag gtcgacgtcc
     2101 tcgcgaagta cgtcgaacgg cttctccggc gcgccgtccc ggccacctcg gcggacgaga
     2161 ggctccgcgc gcttctcggc tcgtgacgac ccgacccggc gcctacgtcc acgtccccta
     2221 ctgtgcgcac cgctgctcct actgctcctt cgtggcggtg acgggccggg agacggagcg
     2281 ggagtacttc gacgccgtcg tcgcggagat cggtgcgcgg gccggcgagg tccccggacc
     2341 gctcgagacc gtctacctcg gcggcggcac gccgtcgttc gccgaccccg gcagcctcgc
     2401 gagggtcctc ggggcgctcg agcggacgtg ggggctcgtc gcggacgccg aggtgaccgc
     2461 cgaggcgaac ccggacgacc tcgtcccgga gcgcctcgac gccctcgtcc gccttggcgt
     2521 gaaccggctc tccgtcgggg tccagtcgct ccgcgacgcc gagctcgccc ctctggagag
     2581 gcggcacgac gccgcgtccg cgcgggacgc ggtccgcctt gctctcaacc ggggcctccg
     2641 ggtctccgcg gacctgatga tcggcattcc gggccagacg cgcgagagcc tcctcgaggg
     2701 cctctcggag ctcctctcgt ccggcgtcgg gcacgtttcg gtgtacctcc tcgagatcga
     2761 gaaggccccg cgcctcgtcg ccctccgaga ttcggccccg agcctcttcg cggcggacga
     2821 cgagatggcg gagcgatggg aacgcgtcga cgacgcctgc cgtgctgcgg gcctcccgcg
     2881 ctacgagacc tcgaactggg cgaggccggg ggaggagagc cgccacaacc tgaagtactg
     2941 gcggcgggag ccggtcgtcg cgttcggcgt gtcggcggcc tccttcgacg gcctcgtccg
     3001 gcgaacgaac ggggggtcga tcccggccta cgtcgcggcg gtccgcgcga ccggaacggc
     3061 gttcgtggcc gaggagcggc tgccgtccga ggcggctctc cgggaggagg tcctcctcgg
     3121 tctccgcacg gtcgtcggag tcgcggccgg gaggttcacc gaggcggtcg acacgctgcc
     3181 gcccgccgac cgcgcgcggc tctcggacgc cgcggctgcg ggcctcctgg agacggagtc
     3241 gggccgggtc cggctgacac gcgccggcgt tctcctctcg aacgaagtct tcgcgctcct
     3301 tctctgagat ccgcgtccgg ggcgcgggag gcgcgcggcg gaccggcccg cggtccgctt
     3361 ggcctgccgg gccgggcgtg agaccatccg ccggctcggg accagaagga cccggattct
     3421 tacgttccgg agcggaggcg catgaacgca gagactctgg tctatgtcat ccccgtgttc
     3481 ggtctcgcgg ggcttctcta cacctggctg aagtcgtcct gggtcgcccg gcaggacgtc
     3541 ggcacggagc ggatggccaa gatcgccggc gcgatccagc agggcgccat ggcgttcctg
     3601 cgggccgagt accgcgtcct cggggtcttc gtcgccgtcg tggccctgct gctcggcatc
     3661 tccggagcgc ttcaggaggg gtcgagcccg ctcatcgcgg cggcgttcgt cgccggagcg
     3721 ctctgctcgg cgctggcggg cctgatcggg atgagggtcg ccacgaaggc gaacgtgagg
     3781 acgacccagg cggcgcgccg gggcctcggg ccggcgctcg agatcgcctt cgcgggcggc
     3841 tcggtcatgg gcctcggggt ggtcggcctc ggcgtcctcg gccttggcgg ctgcttcctc
     3901 gtcttctcga agctcttcgc cggcgacgtc cacaagacga tcgccgtcat tacgggtttc
     3961 tcgttcgggg ccagctcgat cgccctattc gcccgggtcg gggggggcat ctacacgaag
     4021 gcggccgacg tcggcgcgga cctcgtcgga aaggtcgagg cggggatccc cgaggaccac
     4081 ccgctcaatc ccgcgacgat cgccgacaac gtcggcgaca acgtcggcga cgtcgccggg
     4141 atgggcgccg acctcttcga gtcgtacgtc gggtcgatcg tcggggcgat ggtcctcggc
     4201 gcggccttca tgaccggacg gctcgtcacc ggcgagccct tcacggcgga cgggttccag
     4261 ggcctctcgg ccgtcctcct tccgctcgtc ctcgcgggcg tcggcatcct cacctcgatc
     4321 gccggcacgt tcttcgtccg ggtgagggaa gggggatcgc cccagcgcgc cctgaacacg
     4381 ggtgagttcg gctcgtcgat cgtgatggtc gtcctctcct acttcatcat ccgagggatg
     4441 ctccccggga gcttcgtctt cgacgacgtc ctctacggcg cggagcgcac gatcacctcg
     4501 ctcggggtct tcttctcgac cgtcatcggc ctcgccgccg gcctcgccat cggcgtgctc
     4561 acggagcact acaccggcac cgggaccggc ccggtcacct cgatcgcgag gcagtccgtc
     4621 acgggctcgg cgacgaacat catcgcgggc ctcggcgtcg ggatgcgctc gacggcctgg
     4681 ccggtcctga tcctcgccgc ttcgatcatc ggcgccttcc acttcggcgg cctctacggg
     4741 atcgcgatcg cggccgtcgg gatgctctcg aacacgggca tccagctcgc cgtcgacgcc
     4801 tacggcccga tctccgacaa cgcggggggc atcgccgaga tggcggagct cccgaaggac
     4861 gtccggagcc ggaccgacaa gctcgacgcc gtcgggaaca cgaccgcggc gatcggaaag
     4921 gggttcgcga tcgggtcggc cgcgctgacc gccctcgcgc tgttcggcgc cttcatgacg
     4981 gccgcgcacc tgaagacgat cgacgtctcg aaggcggagg tgatggcggg gctcctggtc
     5041 ggggcgatgc tgccgttcct cttctcctcg atggcgatgg ccgccgtggg gcgggcggcg
     5101 atgtcgatga tccaggaggt gaggcgccag ttcagcgaga taccggcgct caaggccgcg
     5161 atcttcgcga tggacgcgca ctccgggaag gacttcaagg actggcccgt ggaggaccag
     5221 aagatcttcg aggacgcgct cgggaaggcc gagtacggca agtgcgtgga gatctccacg
     5281 acggccgcga tcaaggagat ggtcgcgccg ggactccttg cggtcatcgt gccggtcgtc
     5341 gtcggcttcg gaccgaggct cctcggcctc ggctccggcg cggagatgct cggcgggctc
     5401 ctggcgggcg tgacggccac gggcgtcctc ctcgcgatct tccagtcgaa ctcgggaggc
     5461 gcctgggaca acgcgaagaa gatgttcgag gaaggggtcg agatcgacgg tcaccgctac
     5521 ttcaagggct cggaccccca caaggccgcg gtcgtgggcg acaccgtggg tgatcccttc
     5581 aaggacacct ccgggccgtc cctgaacatc ctgctgaagc tcatgtcggt catcgccctc
     5641 gtcatcgcga ccctgctctg agctccgggc gccgcggcgg cgccccttag gtcaccgagc
     5701 gaggccccgg cgagagccgg g
//
LOCUS       DAOTEZ010000281         5711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_10110_length_5711_cov_6.684760, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000281 DAOTEZ010000000
VERSION     DAOTEZ010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5711
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10110_length_5711_cov_6.684760"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="P5164_13875"
     CDS             complement(<1..427)
                     /locus_tag="P5164_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRY45028.1"
                     /translation="MNVLPNPIFIAAIFVVIFFLSKWVNILNEYERAVTFWLGRLAPA
                     PKGPGLTLIFWPFETMVRVDLRTITLDVPPQDVITRDNVSVKVNAVVYFHVMDPARAI
                     VKVANYMYATSQLAQTTLRSILGQAALDELLSEREKLNES"
     gene            complement(424..1716)
                     /locus_tag="P5164_13880"
     CDS             complement(424..1716)
                     /locus_tag="P5164_13880"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HRY45029.1"
                     /translation="MLRIGLATLLLLLAPAALGAGTEVVVVDVDTDINPVTAEFVKRI
                     VEEAAAEGAPAVVLRINTPGGRLDSTREITKSILSSEVPVVGFVHPPGSQAASAGFII
                     LMACDVAAMAPGTNAGAASPVGGGGEELPKTIGKKVSEDAAALVRSLVVPRGRPVDDA
                     VKTITDALSYSESEAKAKGLVEIVARDVPDLLKQLDGRPIKRVGKPDATLASGAFTSR
                     TVSRTPLEKALGVIASPMVAGLLFLIGIVGLYSEFQNPGAILPGVLGSICLLLAFFAM
                     SVLPTNWVGIGLVLLGVLFFFLEVKFTTHGVLAIAGGVSIILGAVLLFHRDEFAPKAE
                     LWFVVGGAVVTTVILAGLSLMALSVQRLPDRTGVAVLVGQVAPVRVGAAGALRVFVDG
                     ALWEARSAAPLTDGQLVEVTGVDGLAVLVKPTARSEKS"
     gene            1737..2651
                     /locus_tag="P5164_13885"
     CDS             1737..2651
                     /locus_tag="P5164_13885"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRY45030.1"
                     /translation="MLQRAVRFALADLLTCGAAALLLAVGLAGGVPASAPAAFAKVAA
                     AFFLPLVFAAVRGRRPAPGSLLASVADFGPILPLLLVFDNLGPFVLGAGSVDLDPLLV
                     AADRLLFGGDDPTRLLEPFLGPPALEVLTVCYALYYFHPIVLGALLWKDDLREKSPGA
                     RFPAYVFGMLLVFYVSYAGYFVVPAIGPRFTVPHDGPLPRGTVAKAIDDTLDRLETSR
                     RNCFPSGHTMVTVAVLVEAARRSRKTFLAFLPFATGLVLATVACRYHYVVDVLAGLVL
                     AFAVGGLARAVARRVRQEADGTVLATSR"
     gene            complement(2612..3760)
                     /locus_tag="P5164_13890"
     CDS             complement(2612..3760)
                     /locus_tag="P5164_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRY45031.1"
                     /translation="MAEGLSVAEVSSRSDLAEFVELPYRLFASEPDWVPPLRSDVRWV
                     LDPAKNPFWRHARRTLFLARRGGRVVGRVAAIADDAHNRVHGDRTAFFGFFECEDDPG
                     AAKALFHAAEGAAGRLLPGCDRLRGPVNPSMNDEVGFLLAGESEPGPPVLMMPWNPAY
                     YLDLVASAGFEKEKDLVALLAPIDDRSFARLGRITDAVRRRNPELTFRTIRMDRFPAE
                     LAKVKEVYNAAWERNWGFVPMTSEEIDAMAKKLKDLLYPPIVWFVESGERPVAFMLGM
                     PDFNQVLIRMGGRLFPFGWLKFLLGRKKVDRVRLMAFGVLPEYRRKGLDAVCYFEGYK
                     AAMAAGFRTVEFSWILEDNLDLLKPIEVFEGRLYRRYRLVARTVPSAS"
     gene            complement(3801..5039)
                     /locus_tag="P5164_13895"
     CDS             complement(3801..5039)
                     /locus_tag="P5164_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HRY45032.1"
                     /translation="MRSSALKPAVAAPVRERVSILDKTRRYTVPDEIKAAGVWTYFRM
                     LESGQDPVVTMDGHEMVMLGSNNYLGLASHPKVKEAAIAAVRKYGAGCAGSRFLNGTL
                     DIHVQLEERLAVFMRKPACITFSTGFLANLGAIGTLPGKGDTIYLDRQDHACIYDGAR
                     LAIGAEVRKFKHNDPDDLVRLLNAHPDKGGKLLVVDGVFSMEGDIAPAPAYAEICAEY
                     GMAFMIDDAHGIGVLGKTGRGTAEHFGIEDRTDLIMGTFSKSLAAIGGFIVADADVVK
                     YIQHKARALIFTAAPPPASIGAVLAALDIIEAEPERRQHLWDNTRFMMTSLRAIGFDC
                     GESETPVIPIVVGEDYLAFKMAKRLEEEGVFVNPVVSPACPPGRALIRTSYMATHRRE
                     HLTRALEAFQKVGRELGLVG"
     gene            5350..>5711
                     /locus_tag="P5164_13900"
     CDS             5350..>5711
                     /locus_tag="P5164_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRY45033.1"
                     /translation="MSVSQLAKSIAESPTLRLNEQARLLREKGEAVVHLGAGEPKNKA
                     PIAAILAAASKLNTGDVKYTPADGTPGLKKQIIRYTEENYDRVVAPENVIVSAGAKQS
                     VFNILYSVLNPQDEVVILA"
BASE COUNT          845 a         2044 c         1956 g          866 t
ORIGIN      
        1 ggctctcgtt cagcttctcg cgctcggaga ggagctcgtc gagggcggcc tgacccagga
       61 tggaacggag cgtcgtctgg gcgagctgcg aggtggcgta catgtagttc gcgaccttga
      121 cgatggctcg ggccgggtcc atgacgtgga agtagacgac ggcgttcacc ttgaccgaga
      181 cgttgtcccg cgtgatgacg tcctgcggcg ggacgtcgag cgtgatcgtc cgcaggtcga
      241 ctcggaccat cgtctcgaac ggccagaaga tgagcgtcag gcccgggcct ttcggcgccg
      301 gcgcgaggcg gccgagccag aacgtgacgg cccgctcgta ctcgttgagg atgttcaccc
      361 acttcgacag gaagaagatg acgacgaaga tcgccgcgat gaagatcggg ttcggaagca
      421 cgttcatgac ttctcgctcc gtgccgtggg cttgacgagg accgccagac cgtcgacgcc
      481 ggtgacctcg acgagctgcc cgtccgtgag gggtgcggcg ctccgggcct cccagagcgc
      541 tccgtcgacg aagactctca gggcgcccgc ggcgccgacg cggaccggcg ccacctggcc
      601 gacgaggacc gcaacgccgg tccggtcggg gaggcgctgg acggaaaggg ccatgaggga
      661 gaggcccgcg aggatgacgg tcgtcacgac cgccccgccc accacgaacc agagctcggc
      721 cttcggcgcg aactcgtcgc ggtggaacag caggacggcc ccgaggatga tcgagacgcc
      781 gccggcgatc gcgaggacgc cgtgggtcgt gaacttcacc tcgaggaaga agaagaggac
      841 cccgaggagg acgagcccga tcccgaccca gttcgtcggc agtacggaca tcgcgaagaa
      901 cgcgaggagg agacagatgc tcccgaggac gcccggaagg atcgccccgg ggttctggaa
      961 ctcggagtag aggccgacga tgccgatcag gaagaggagt cccgcgacca tcgggctcgc
     1021 gatgacgccg agcgccttct cgagcggcgt cctcgaaacc gtccgcgagg tgaacgcccc
     1081 gctcgcgagc gtcgcgtcgg gcttccccac ccgcttgatc ggcctcccgt cgagctgctt
     1141 caggaggtcg gggacgtccc gggcgacgat ctcgacgagc cccttcgcct tcgcctcgga
     1201 ctccgagtag gagagcgcgt cggtgatcgt cttcaccgcg tcgtccacgg gcctcccgcg
     1261 cgggacgacg agcgaccgga cgagggcggc ggcgtcctcg ctcaccttct tgccgatcgt
     1321 cttcggaagc tcctcgcccc cgccgccgac cggagaggcg gcgccggcgt tcgtcccggg
     1381 cgccatcgcc gcgacgtcgc aggccatcag gatgatgaag cccgccgagg cggcctgaga
     1441 cccgggcggg tggacgaagc cgacgaccgg gacctccgag gagaggatcg acttcgtgat
     1501 ctcccgggtc gagtcgagcc gcccgcccgg ggtgttgatc cggaggacga cggcgggggc
     1561 cccctcggcg gccgcctcct ccacgatccg cttcacgaac tcggcggtga cggggttgat
     1621 gtccgtgtcg acgtcgacga cgacgacctc cgtccccgcc ccgagcgcgg ccggagcgag
     1681 cagaaggagc agggtcgcga ggccgatccg aagcatcggc cgattatagg ttcggcgtgc
     1741 tacaacgcgc cgtgcgcttc gcgctcgccg acctgctgac gtgcggggct gcggccctcc
     1801 ttctcgccgt cgggctcgcc ggcggcgtcc cggcctcggc gccggccgcc ttcgcgaagg
     1861 tcgcggccgc gttcttcctc ccgctcgtct tcgccgcggt ccggggccgg cgccccgccc
     1921 ccgggagcct cctcgcgagc gtcgccgact tcgggccgat cctgccgctc ctcctcgtct
     1981 tcgacaacct cggcccgttc gtcctcggtg ccggctccgt cgacctggat cccctcctcg
     2041 tcgcggcgga ccggctcctc ttcggcggcg atgacccgac gcggctcctc gagccgttcc
     2101 tggggcctcc cgccctcgag gtcctcaccg tctgctacgc cctctactac ttccacccca
     2161 tcgtcctcgg tgcgctcctc tggaaggacg acctccggga gaagagcccc ggcgcgcgct
     2221 tccccgccta cgtcttcggg atgctcctcg tcttctacgt cagctacgcc ggctacttcg
     2281 tcgtgccggc gatcggaccc cgcttcacgg tgccgcacga cggtcccctt ccccgcggga
     2341 cggtcgcgaa ggcgatcgac gacaccctag accgtctcga gacgagccgg cggaactgct
     2401 tcccttctgg gcacacgatg gtgacggtcg cggtcctcgt cgaggcggcc cggcggtcgc
     2461 ggaagacgtt cctcgccttc cttcctttcg cgaccggcct cgtcctggcc acagtcgcct
     2521 gccggtacca ctacgtcgtc gacgtcctcg cgggcctcgt cctcgcgttc gcggtcggcg
     2581 gcctcgcccg ggcggtcgcc cggagggtcc gtcaggaagc cgacgggacg gtcctggcga
     2641 cgagccggta gcgcctgtag agcctcccct cgaagacctc gatcggcttc agcaggtcga
     2701 ggttgtcctc gaggatccag gagaactcga ccgtcctgaa gccggccgcc atcgccgcct
     2761 tgtagccctc gaagtagcag acggcgtcga gccccttccg ccggtactcg ggcaggaccc
     2821 cgaacgccat gaggcggacc cggtcgacct tcttccggcc gaggaggaac ttcagccacc
     2881 cgaacgggaa gaggcgaccg cccatccgga tcaggacctg gttgaagtcg ggcatgccga
     2941 gcatgaacgc gaccggcctc tcgcccgact cgacgaacca gacgatcggc gggtagagga
     3001 ggtccttcag cttcttcgcc atcgcgtcga tctcttccga cgtcatcggc acgaagcccc
     3061 agttcctctc ccaggccgcg ttgtagacct ccttgacctt cgcgagctcc gccgggaaac
     3121 ggtccatccg gatcgtccga aacgtcagct cgggattcct ccggcggacc gcgtcggtga
     3181 tccggccgag gcgggcgaag gaacggtcgt cgatcggcgc gaggagggcg acgaggtctt
     3241 tctccttctc gaagccggcc gaggccacga ggtcgaggta gtacgccggg ttccagggca
     3301 tcatcaggac cggcggcccc ggctcgctct cgcctgcgag gaggaagccg acttcgtcgt
     3361 tcatcgacgg gttgaccggc ccccgaaggc ggtcgcagcc cggcaggagc ctccccgccg
     3421 ccccctcggc ggcgtggaag agcgccttcg ccgcccccgg gtcgtcctcg cattcgaaga
     3481 agccgaagaa tgccgtccgg tcgccgtgga ctcggttgtg tgcgtcatcg gcgatcgcgg
     3541 cgacgcggcc gacgacccgg ccgccgcgcc gggcgaggaa gagggtccgc cgggcgtgtc
     3601 tccagaaggg gttcttcgcc gggtcgagga cccagcggac gtccgaccgg agcggcggca
     3661 cccagtcggg ctcggacgcg aagagccggt acggaagctc gacgaactcc gcgaggtcgc
     3721 tccgggagga gacctcggcg acggacagcc cttcggccac gctccggtcc ctcccgctcc
     3781 ggcccggccc cggtcggcgc ctagccgacg aggccgagct cgcgcccgac tttctggaac
     3841 gcctcgaggg cccgcgtcag gtgctcccgc cggtgcgtcg ccatgtacga cgtccggatg
     3901 agcgcccgtc ccggcgggca ggcggggctg acgacggggt tcacgaagac cccctcttcc
     3961 tcgagccgct tggccatctt gaacgcgagg tagtcctctc cgacgacgat cgggatgacc
     4021 ggcgtctccg actcgccgca gtcgaagccg atcgcgcgga ggctcgtcat catgaagcgg
     4081 gtgttgtccc agaggtgctg ccgccgctcc ggctccgcct cgatgatgtc gagcgcggcg
     4141 aggacggcgc cgatcgatgc cggagggggc gccgccgtga agatgagcgc ccgcgccttg
     4201 tgctggatgt acttgacgac gtcggcgtcg gccacgatga agccgccgat ggccgccagc
     4261 gacttcgaga acgtccccat gatcaggtcc gtgcggtcct cgatgccgaa gtgctccgcc
     4321 gtcccgcgtc cggtcttgcc gaggacgccg atgccgtggg cgtcgtcgat catgaacgcc
     4381 atgccgtact cggcgcagat ctcggcgtag gccggcgccg gggcgatgtc gccctccatc
     4441 gagaagacgc cgtccacgac cagcagcttc ccgcccttgt cggggtgggc gttcaggagc
     4501 cggacgaggt cgtcggggtc gttgtgcttg aacttccgga cctccgcccc gatggccagg
     4561 cgcgccccgt cgtagatgca ggcgtggtcc tggcggtcga ggtagatcgt gtcgcccttc
     4621 ccggggaggg tcccgatcgc ccccaggttc gcgaggaacc cggtggagaa cgtgatgcag
     4681 gccggtttcc gcatgaacac ggccaggcgc tcctcgagct gcacgtggat gtcgagcgtg
     4741 ccgttcagga accggctgcc cgcgcagccg gcgccgtact tcctcaccgc cgcgatcgcg
     4801 gcttccttga ccttcggatg ggaggccagg ccgaggtagt tgttcgaccc cagcatcacc
     4861 atctcgtggc cgtccatggt gaccaccggg tcctgccccg attcgagcat ccggaaatag
     4921 gtccagacgc cggccgcctt gatctcgtcg ggcaccgtgt accggcgcgt cttgtccagg
     4981 atcgagaccc gctcccgaac aggggcggcg accgcgggct tgagcgcgga actcctcata
     5041 ctcctccgcc agcgtcgccg cccaccgggg ccttccgggc gacccgcaaa cgtcgccgat
     5101 cttagcaccc gctcctcccc cgccccgagg cggtccccgg aggccccgtt ccggcccacg
     5161 gaagcgggac ggtcccgcgg ggaccggctc gcgccgcccg gacgcggctc cgtcccgggg
     5221 agccggtccg gtggtgcgct tcccgcccgg ggcgggaacg cctccccgcc ggtacaatcc
     5281 ggcgttgccc tcggcgtcgc ggagcacggc tccagcggcc caccggcgaa aagggaggtt
     5341 cccaacgaca tgagcgtaag tcagctcgcg aagtccatcg cggagtcgcc gacgctccgc
     5401 ctgaacgagc aggcccgtct cctgcgcgag aagggcgagg cggtcgtcca tctcggcgcg
     5461 ggcgagccga agaacaaggc tccgatcgcc gcgatcctcg cggctgcgtc gaagctgaac
     5521 accggcgacg tgaagtacac gccggcggac ggcacgccgg ggttgaagaa gcagatcatc
     5581 cggtacacgg aggagaacta cgaccgcgtc gtcgcccccg agaacgtcat cgtgtcggcc
     5641 ggggcgaagc agtcggtctt caacatcctc tactcggtcc tgaacccgca ggacgaggtc
     5701 gtcatcctcg c
//
LOCUS       DAOTEZ010000282         5686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_10149_length_5686_cov_6.913692, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000282 DAOTEZ010000000
VERSION     DAOTEZ010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10149_length_5686_cov_6.913692"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="P5164_13905"
     CDS             complement(<1..225)
                     /locus_tag="P5164_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005984288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRY45034.1"
                     /translation="MKLPALVSFTAGLVPALAAGWLLFPRLIYKTEAQPLPFNHKVHA
                     EQGMACGDCHAVAEDGRFAGMPTTESCAGCH"
     gene            complement(222..1169)
                     /locus_tag="P5164_13910"
     CDS             complement(222..1169)
                     /locus_tag="P5164_13910"
                     /inference="COORDINATES: protein motif:HMM:NF013743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45035.1"
                     /translation="MRCPFLREAHVQSCQASPIRKQIVRTGSASDERCATAAWHECPV
                     VRELNEEHPSASSCPFRTESLAQYCSAAPVTKLIPWSDQAFSRCGSEAHRYCDLYLAL
                     ARTPAPTAAPGEAPPRTDDADGIPVPEWLWFAPGHTWLSVASDGVCHIGVDAFLARTL
                     GGIDRVQFVTTKGLERPAAVLTVRDVDVTVSFPNPVFITAVNGALRADPARLARDPYG
                     LGWLFEGIVRDRAAVPHGAPRITDGLLRGEAATSWMRRETLRLRGLAEDAAERISGVR
                     LAADGGLPEPALVRTLPHEEALRLSHLLLSTASDEERST"
     gene            complement(1195..1431)
                     /locus_tag="P5164_13915"
     CDS             complement(1195..1431)
                     /locus_tag="P5164_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45036.1"
                     /translation="MQPTDSEILWLNLTNAGLGLATLGGIVALGWAAVRELRERAKGR
                     VAVHDLDPHAAFIPELGLTMADGGEKVDENEEKK"
     gene            complement(1659..2984)
                     /locus_tag="P5164_13920"
     CDS             complement(1659..2984)
                     /locus_tag="P5164_13920"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY45037.1"
                     /translation="MRKLWAVLKREYRETVRKPSFLIMTVLAPFLMGALMLVPAFLAS
                     RGMGERRVAVLDGTGRLRAAVEGLAGEAPGERSAFARGRGTNVPTGRIVPEYVDLFTV
                     DPKNAVGPYLERLSVERTPKERRLDGVLLVPADAFERPLTHLTYFSRSAADLLAQERL
                     GRAVNRALQRERLAARGVAPAELELILAPLPVETVQVTKSGQERRGGEGSFVLAMLFL
                     ALLFIPSLVYGQEVMRGVIQEKTDRVVELLVSSMTPMELLSGKILGMAAIGLTQMAVW
                     VAMGGAVAAAGLSEARSAGFDLSSVLRPSIAVWFVVFFLLSYLVTVGVYAAGGAIVSS
                     EKEAQQVLTPVMFVFLVPWFLMMPILTNPDSTLSVVLSLVPIYTPMTMFIRLLVSEPP
                     AWQVALSLVLSVATIAGLLKASAKVFRAGLLATGKRPTIPELWRWLKEA"
     gene            complement(2981..3898)
                     /locus_tag="P5164_13925"
     CDS             complement(2981..3898)
                     /locus_tag="P5164_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY45038.1"
                     /translation="MSRVLSLHDVVKHYGSVKAVDGVSFEVNAGKITGLLGRNGAGKT
                     TTLRMITGVLRPDRGRVELFGAAVEGARDRLGYLPEERGLYRKMRVLDHLLFLAEIKG
                     RSPASMRPAAERWLKRFELSEKKDSKVEELSKGNQQKVQLAGALLFDPELVVLDEPGS
                     GLDPVNVVLVRRLLRELAAEGRAILLSTHQMGEAEKLCDEIVLVHAGMVVRSGPLAEV
                     KASGGRDAVHLEFTGDGAFLAEHPAVASARIDTNRAELSLAPGADAQELLAAAAVRLR
                     VLRFEVVAPSLEEVFLEAVGEAAPPEVAA"
     gene            3963..4520
                     /locus_tag="P5164_13930"
     CDS             3963..4520
                     /locus_tag="P5164_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45039.1"
                     /translation="MNRRASAPALLAMLVLGSCASGGGTDPTLVEAGRKLRREQPPPT
                     AAELRLEVDSFLENELPLLRKRAVQRIEREAKVWRATFVGGAALGVLAASSGSIAGSG
                     DAAKASLVGAGGAAAILGGVGYYVRTPRHQACITFLDAAHADLSSWRSHAIPPGDGTV
                     APAVWHAWVDRVASIRAAAPCAKSR"
     gene            4583..5326
                     /locus_tag="P5164_13935"
     CDS             4583..5326
                     /locus_tag="P5164_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY45040.1"
                     /translation="MDGGVALVTGASRGIGAATASLLARSGAKVCVNYATNEEAARSV
                     VAGIEAEGGTALAVRADVTDRDAVERMVATAERELGPIDILVANAGMRVHLAPFLGYP
                     WEELEAKLVGEVKAAFFAAQAVAPSMAARGGGAIVVVSSTLSRRPGEGFVAHSAAKAA
                     LDGFVRALALELGPRGIRVNTVAPGLTLTDATAFLPEPAKAAAAQATSLRRNAAPEDV
                     AGAILALVSDASRHVTGVWLPVDGGVTMP"
     gene            complement(5339..>5686)
                     /locus_tag="P5164_13940"
     CDS             complement(5339..>5686)
                     /locus_tag="P5164_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRY45041.1"
                     /translation="VLAARAHSLPALDGVHLALEDEEGFAAACRQGRDLGFDGKTLIH
                     PRTIPAANRAFAPDPAEVDDARRLLAAWRAARAEGRGVVVHERRLVEELHAKEAERLV
                     ALAQAIAARDAGG"
BASE COUNT          812 a         1982 c         2147 g          745 t
ORIGIN      
        1 gtgacacccg gcgcagctct cggtcgtcgg catgccggcg aagcggccgt cctccgcgac
       61 ggcgtggcag tcgccgcagg ccattccctg ctcggcgtgg accttgtggt tgaagggaag
      121 gggctgagcc tcggtcttgt agatcaggcg cgggaagagg agccagcctg ccgccagggc
      181 ggggacgagg ccggctgtga acgagacgag ggccggtagc ttcaagtaga cctctcttcg
      241 tcggaagcgg tggagaggag gaggtgggaa agccggagcg cctcctcgtg gggaagggtc
      301 cggacgaggg ccggctcggg gagaccgccg tcggcggcga ggcgcacgcc cgagatccgc
      361 tccgcggcgt cctcggcgag cccgcgcagg cgcagcgtct cgcggcgcat ccaggaggtc
      421 gcggcctcgc cgcgaaggag accgtcggtg atccgcgggg cgccgtgagg gacggcggcc
      481 cggtctcgga cgatcccctc gaagagccag ccgaggccgt agggatcgcg ggcgaggcgc
      541 gcgggatccg cgcggagcgc gccgttgacg gcggtgatga agaccgggtt cgggaaggag
      601 acggtcacgt ccacgtcgcg gacggtgagg acggccgccg gcctctcgag ccccttcgtc
      661 gtgacgaact gcacccggtc gatcccgccc agcgtccgcg cgaggaacgc gtcgaccccg
      721 atgtggcaga cgccgtcgga cgcgaccgag agccaggtgt gccccggcgc gaaccagagc
      781 cactccggga ccgggatgcc gtcggcgtcg tcggtccggg gcggggcctc gccgggggcc
      841 gcggtcgggg cgggggtcct cgcgagcgcc aggtagaggt cgcagtagcg gtgcgcctcg
      901 cttccgcagc gggagaaggc ctggtcgctc caggggatca gcttcgtcac cggcgccgcg
      961 gagcagtact gggcgagcga ctccgtccgg aacggacagg agctcgcgct cgggtgctcc
     1021 tcgttcagct cgcggacgac ggggcactcg tgccaggctg ccgtcgcgca gcgctcgtcc
     1081 gatgcgctcc ccgtccgcac gatctgcttg cggatcgggg aggcctggca cgactggacg
     1141 tgcgcctcgc gaaggaaggg gcaacgcacg gcttcgcctt tcgcagcccc ggggctactt
     1201 cttctcctcg ttctcgtcga ccttctcgcc gccgtcggcc atcgtcaggc cgagctcggg
     1261 gatgaaggcg gcgtgcgggt cgaggtcgtg aacggcgacg cggcccttcg cgcgctcgcg
     1321 gagctcgcgg acggccgccc agccgagagc gacgatcccg cccagggtag cgaggccgag
     1381 tccggcgttc gtcaggttga gccagaggat ctcggagtcg gtgggctgca tgggaaacct
     1441 cctcgcgtcg ggatttccct cgctgggaag tcgacgcgac atgggaggtg caggaggcat
     1501 gccgactccc tccggagtca ggccgacgag cgtaaacgac tcgtatagtt gtatttgctc
     1561 tggatgcccc ggatgggcga gcgtgcgacg tggtccgcga gtgccggatt tcccgacggg
     1621 cgcgtcggag ttcccgacgc gcccgttccg acttcagctc acgcttcctt cagccagcgc
     1681 cagagctcgg ggatcgtcgg ccgcttgccg gtcgcgagaa ggcctgcccg gaagaccttg
     1741 gcggaggcct tcagcaggcc cgcgatcgtc gcgaccgaga ggacgagcga gagggcgacc
     1801 tgccacgccg gcggctcgga gacgaggagc cggatgaaca tcgtcatcgg cgtgtagatc
     1861 gggacgagcg agaggacgac ggagagcgtc gagtcggggt tcgtcaggat cggcatcatc
     1921 aggaaccacg gaacgaggaa gacgaacatc accggcgtga ggacctgctg ggcctccttc
     1981 tccgagctga cgatcgcccc gccggcggcg tagacgccga cggtgacgag gtaggagaga
     2041 aggaagaaga cgacgaacca gacggcgatc gaagggcgga ggacgctcga gaggtcgaag
     2101 cccgcggagc gcgcctcgga aaggccggcg gcggcgacgg cgcctcccat cgccacccag
     2161 acggccatct gcgtcaggcc gatcgccgcc atcccgagga tcttcccgga gaggagctcc
     2221 atcggcgtca tcgacgagac gaggagctcg acgacgcggt ccgtcttctc ctggatgacg
     2281 ccccgcatca cctcctgccc gtagacgagc gatgggatga agaggagggc gaggaagagc
     2341 atcgcgagga cgaagctccc ctctccgccc cggcgctcct gccccgactt cgtcacctgg
     2401 acggtctcga ccggcagcgg cgcgaggatc agctccagct ccgccggcgc aactccgcgc
     2461 gcggcgaggc gttcccgctg gagggcgcgg ttcacggcgc gcccgaggcg ctcctgggcg
     2521 aggaggtcgg ccgcggagcg gctgaagtag gtgaggtggg tgaggggccg ctcgaaggcg
     2581 tcggccggga cgaggaggac cccgtcgagg cgccgctcct tcggcgtccg ctcgacggag
     2641 agtcgctcga ggtagggacc gaccgcgttc ttcgggtcga ccgtgaagag gtcgacgtac
     2701 tcggggacga tccggccggt cgggacgttc gtcccccggc ctcgggcgaa ggccgaccgc
     2761 tcgccgggcg cctcccccgc gagcccctcg acggcggcgc gcagccgccc cgtgccgtcg
     2821 aggaccgcga cccgccgttc gcccatcccg cgcgaggcga ggaacgcggg gacgagcatc
     2881 agcgccccca tgaggaacgg ggcgaggacg gtcatgatca ggaacgaggg cttgcggacg
     2941 gtctcgcggt actcgcgctt caggacggcc cagagcttcc tcacgccgcc acctccggag
     3001 gcgcggcctc tcccaccgcc tcgaggaaga cctcctccaa ggagggggcg acgacctcga
     3061 agcggaggac cctgaggcgc accgcggcag cggcgaggag ctcctgcgcg tcggcgccgg
     3121 gggcgaggct cagctcggcc cggttcgtgt cgatccgggc ggacgcgacg gcggggtgct
     3181 ccgcgaggaa cgcgccgtct cccgtgaact ccaggtggac ggcgtcccgc ccgcccgagg
     3241 ctttcacctc ggcgagcggc cccgagcgga cgaccatccc ggcgtggacg aggacgatct
     3301 cgtcgcagag cttctccgcc tcgcccatct ggtgcgtcga gaggaggatg gcgcgcccct
     3361 ccgcggccag ctcgcggagg agccgcctca cgaggacgac gttcaccggg tcgagacccg
     3421 agccgggctc gtccaggacg acgagctccg ggtcgaagag gagcgcgccc gcgagctgga
     3481 ccttctgctg gttccccttc gagagctctt ccaccttgct gtccttcttc tcggacagct
     3541 cgaagcgctt cagccaccgc tcggcggcgg gcctcatcga ggccggagag cgccccttga
     3601 tctcggcgag gaagaggagg tggtcgagga cgcgcatctt tcggtagagg ccgcgctcct
     3661 ccgggaggta gccgagccgg tcgcgagccc cctcgaccgc cgcgccgaag agctccaccc
     3721 ggccgcgatc gggccgcagg acgcccgtga tcatacggag cgtcgtcgtc ttgccggcgc
     3781 cgttccggcc gaggagcccg gtgatctttc cggcgttcac ttcgaaggag acgccgtcga
     3841 cggctttcac cgaaccgtag tgcttcacga cgtcgtggag ggagaggacg cggctcacgg
     3901 ggcggcatct tacgcacccc ggaacgagcg gtgcgaagcg tatcctcggg agccgagccg
     3961 ccatgaaccg ccgcgcctcc gcaccggccc tcctcgccat gctcgtcctc ggctcctgcg
     4021 cctcgggagg agggaccgat ccgaccctcg tcgaggcggg ccgcaagctc cggcgggagc
     4081 agccgccgcc caccgcggcc gagctccggc tcgaggtcga ctcctttctc gagaacgagc
     4141 ttccgctcct gcggaaaagg gccgtgcagc gcatcgagcg ggaggcgaag gtctggcggg
     4201 cgacgttcgt cggcggggcg gcgctcggag tcctggccgc gtcgtccggg agcatcgccg
     4261 gctccggcga cgccgccaag gcatccctgg tcggcgccgg cggagccgcc gcgatcctcg
     4321 gcggcgtcgg gtactacgtg cggacgccga ggcaccaggc ctgcatcacg ttcctcgacg
     4381 cggcgcatgc cgacctctcc tcctggcgga gccacgccat acctcccggc gacgggaccg
     4441 tcgctcccgc cgtgtggcac gcgtgggtcg atcgggtggc gtcgatccgc gccgccgcgc
     4501 cctgcgcgaa gagccgctga cccggctggt acactcgcgg gggctggcgg cgaagccgcg
     4561 agcggaggta acggacgtga gaatcgacgg cggggttgcg ctcgtgacgg gcgcgagcag
     4621 gggcatcggc gcggcgacgg cgagcctcct cgcccggagc ggcgcgaaag tctgcgtgaa
     4681 ctacgccacg aacgaggagg ccgcgcgcag cgtcgtcgcg ggcatcgagg cggagggcgg
     4741 aacggcgctc gccgtccggg ccgacgtgac cgaccgggac gccgtggagc gcatggtcgc
     4801 gacggccgag agggagctcg ggccgatcga catcctcgtc gcgaacgccg ggatgcgggt
     4861 ccacctcgcg cccttcctcg gctacccgtg ggaggagctc gaggcgaagc tcgtggggga
     4921 ggtcaaggcc gccttcttcg cggctcaggc cgtcgcgccg tcgatggccg cgcggggcgg
     4981 aggcgcgatc gtcgtcgtct cgagcaccct ctcccgccgc ccgggcgagg gcttcgtcgc
     5041 tcacagcgcc gccaaggcgg ccctcgacgg cttcgtccgg gccctcgccc tcgagctcgg
     5101 gccgcgcggt atccgcgtca acaccgtggc gcccgggctg acgctgaccg acgcgacggc
     5161 cttcctcccg gagccggcta aggcggcggc ggcgcaggcg acgtctctcc ggaggaacgc
     5221 ggcccccgag gacgtcgccg gcgcgatcct cgcgctcgtc agcgacgcgt cgcgccacgt
     5281 caccggcgtc tggctcccgg tcgacggcgg cgtcacgatg ccctgactcc cggcgccgtc
     5341 agcccccggc gtctcgcgcg gcgatcgcct gcgcgagcgc gacgagtcgc tcggcctcct
     5401 tcgcgtggag ctcctcgacg aggcgccgct cgtggacgac gacgccccgc ccttccgccc
     5461 gcgcggcgcg ccacgccgcg aggagcctgc gggcgtcgtc cacttcggcc gggtcggggg
     5521 cgaacgcgcg gttcgcggcg gggatcgtgc gcgggtggat cagcgtcttg ccgtcgaagc
     5581 cgaggtcccg cccctgccgg caggccgccg cgaacccctc ctcgtcctcg agggcgaggt
     5641 ggacgccgtc gagcgccgga agggaatgcg cccgggccgc gaggac
//
LOCUS       DAOTEZ010000283         5652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_10249_length_5652_cov_6.350724, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000283 DAOTEZ010000000
VERSION     DAOTEZ010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10249_length_5652_cov_6.350724"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="P5164_13945"
     CDS             complement(<1..1180)
                     /locus_tag="P5164_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45042.1"
                     /translation="MTLRRAAALLLGLLAAGGLASALAARLAPLPPGAEERRASALEE
                     RARRALKRDAGRLAGVAEKLASDPALAEIVEGGTAGVRPGGLYRLLGASLPKGPGWGA
                     VLLDRSGDAVAWAGEPGDLPDAAAPRAGPFAASFRVTEGTLAHESRLGRSPETQGLLI
                     VTRRFPTGIVRPDLLDASRGAAASTQRRVRVRAARSPDRLLAVSLEPGTRAVVEEDVR
                     TSSSRSGALLCGVAAVALGLLARRPAAGAVAARLVLLLAVPHADDGPFARILADPTGL
                     LATPFDAAFTGVMALVLLRSALGDARRVAGATGRLAAAAAGALAACAPAVLGLAGGLA
                     VPSLLLGLGLFAGPPEAPLASAGFVAVATSLLGLGALLAGQALRAGRGTAAAGAVGLV
                     L"
     gene            complement(1177..2859)
                     /gene="hutU"
                     /locus_tag="P5164_13950"
     CDS             complement(1177..2859)
                     /gene="hutU"
                     /locus_tag="P5164_13950"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HRY45043.1"
                     /translation="MSTVSAPARYAPPALPRGTTLRCRSWLTEAPYRMLLNNLDPDVA
                     ERREDLVVYGGSGKAARNPECLEAILRELERLGDDETLLVQSGKPVAVFRTHADAPRV
                     LIANSNLVPKWGTWDEFRRLEALGLTMYGQMTAGSWIYIGTQGILQGTYETFAEAGRR
                     DLGSPDGSLAGKLAVTAGLGGMGGAQPLAVTMGGGTALVAEVDAHRIQRRLETRYCDE
                     RIDDLDSAIDRALAARDAKEGISIGVLANAVDLLARLVARGVVPDLLTDQTSAHDELN
                     GYVPHGISYEAALALRKSDPGEYGRRAVASMGEHVRLMLELQRRGAVTFDYGNNIRAQ
                     AVKAGVTNAFEIEGFVPKYIRPLFCLGKGPFRWAALSGDPADIAATDRALVELFPENA
                     ALHRWLEMAAKRVAYQGLPARICWLGYGEREKAGLAFNELVRSGKVKAPIVIGRDHLD
                     CGSVASPNRETESMKDGSDAIADWPLLNALVNTAAGATWVSIHHGGGVGIGYSMHAGM
                     VVVADGTEAAARRLSRVLVSDPGMGVVRHVDAGYPEAVACSEAHGVAIPMRR"
     gene            2943..3695
                     /locus_tag="P5164_13955"
     CDS             2943..3695
                     /locus_tag="P5164_13955"
                     /inference="COORDINATES: protein motif:HMM:NF019494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HRY45044.1"
                     /translation="MLLDIHYAARQIVRAMTSRHREIVRYRNRKLYEKSERRFVTLDD
                     IARAVAGGDRIRVVSADTGEDITAKILSRALASDRVAFPASTDALALLLRAGSDAAET
                     VAGVAERVGAARMAGAVRKAAQPERIAETFAPVTRRVETARHDVERIVAGLVGRGRLT
                     WEEGARLREDVGTVFRESLSDVVGRVRDLVRRVPPASSPAIHAEIQELARRLDHLEAL
                     AAASFPKGSSAAPRSEYAARPAPARKKETRKP"
     gene            3692..4177
                     /locus_tag="P5164_13960"
     CDS             3692..4177
                     /locus_tag="P5164_13960"
                     /inference="COORDINATES: protein motif:HMM:NF017415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HRY45045.1"
                     /translation="MTRKTTRRAAPAAKPLPFDVPPQIDRALRTAVEKVDAVRDETRT
                     VASKVEKTVRAAATSAKKTGETLARDPKRFVEGVVRDAKSNLKTVGADVSREAGRVAD
                     EVARRVSAVVEPALEKALHRLNVPTQKDLKSLVAKVDALGRKMEQLHAPARKPRSRTR
                     A"
     gene            4253..5452
                     /locus_tag="P5164_13965"
     CDS             4253..5452
                     /locus_tag="P5164_13965"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRY45046.1"
                     /translation="MAAPRLRLVSSRGPSSPRPLSSRRGLVCAGGGITGAIYEIGVLA
                     ALEDRLDGFSLTDCDVFVGVSAGAYVGALVANGTSPSVLYRNATHPRGEGLDRLSFYR
                     PNLGEIASRVVSAPRIVLEALRDLRDSPGDLTLTDLVASLAGLLPSGFFLLDGLEAWL
                     AEWLSHPGRTNDFRALRRDLRLVAVALDSGETRVFGSSRDEAVPISRAVAASCALPGF
                     FRPVRIDGVDYIDGGVRKTAHISLALKERCGLVLCVNPIVPLRYPLAPSLSPRYPGQG
                     ALAGRGLLSVLDQVFRLSLHSRLRYGLDRYRRDYPDADIVLFEPKAEELPRLMRNMMR
                     TSGRVRIAEYAYRTTQQKLDAEYGRLSRVFERHGLRLRPATSVRRALRRGTVPRPAAA
                     RAPSTRR"
     gene            complement(5439..>5652)
                     /locus_tag="P5164_13970"
     CDS             complement(5439..>5652)
                     /locus_tag="P5164_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014378158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRY45047.1"
                     /translation="PTFPLLGPLVLVPAPTKWWIDVGAPIDAGLDPAYAERPMIVNEV
                     TDLVRSTIQRMIDSRLARRGNVFTGE"
BASE COUNT          759 a         2163 c         2012 g          718 t
ORIGIN      
        1 cgaggacgag cccgaccgcg ccggccgccg ccgtcccgcg tccggcgcga agcgcctgtc
       61 ccgcgaggag cgccccgagg cccaggaggc tcgtcgcgac ggccacgaag ccggcggagg
      121 cgagaggcgc ttccggcggt cccgcgaaga ggccgaggcc gaggaggagc gacggaaccg
      181 cgaggccacc ggcgaggccg aggacggccg gcgcgcacgc cgccagcgcc cccgccgcgg
      241 cggcggccag ccggccggtc gccccggcca cccgtcgcgc atctccgagc gccgatcgta
      301 ggagaacgag cgccatcacc ccggtgaagg cagcgtcgaa cggcgtggcg aggaggccgg
      361 tcggatccgc caggatccgg gcgaagggac cgtcgtcggc atggggcacg gcgagcagga
      421 ggacgagccg cgccgcgacg gccccggccg ccggtcgacg ggccaggagc ccgagcgcga
      481 ccgccgcgac gccgcagagc agcgcgccgg aacgcgacga ggaggtccgg acgtcctcct
      541 ccacgaccgc cctcgtgccc ggctcgaggc tcaccgcgag gaggcggtcc ggcgatcggg
      601 ccgcgcggac gcggacccga cgctgcgtgg aagcggccgc gccgcggctc gcgtccagga
      661 ggtccggccg gacgattccc gtcgggaagc gccgggtcac gatcaggagc ccctgcgtct
      721 ccgggcttcg accgagccgg gactcgtgcg cgagcgtccc ttccgtcacc cggaacgacg
      781 ccgcgaaggg accggcccgc ggtgcggccg catccggcag gtcccccggc tcgccggccc
      841 acgccacggc gtcgccgctc cggtccagaa ggacggcgcc ccacccgggc cctttcggga
      901 gggacgcgcc gaggaggcgg tagagcccgc ccggcctcac cccggccgtc cccccttcga
      961 cgatctcggc gagggccggg tcggacgcga gcttttccgc gacgccggcg agccgccctg
     1021 cgtcgcgctt cagcgcgcgg cgcgctcgct cctcgagcgc gctcgcgcgg cgttcctccg
     1081 ccccgggcgg aaggggcgcg agacgcgcgg ccagcgccga ggcgagcccc cccgcggcga
     1141 ggaggccgag caggagggcg gccgcgcgtc ggagagtcaa cgccgcatcg gaatcgcgac
     1201 gccgtgcgcc tcggagcagg cgaccgcctc cggatacccg gcgtcgacgt gccggaccac
     1261 gcccatcccc ggatcggaga cgaggacgcg cgaaaggcgg cgcgccgcgg cctcggtccc
     1321 gtcggccacg acgaccatcc ccgcgtgcat cgagtagccg atgccgacgc ctcccccgtg
     1381 gtggatcgag acccacgtcg cgccggccgc cgtgttcacg agggcgttca ggagcggcca
     1441 gtcggcgatc gcgtcgctgc cgtccttcat cgattccgtc tcgcggttcg gcgaggcgac
     1501 cgagccgcag tcgagatggt cgcggccgat gacgatcggc gccttcacct ttccgcttcg
     1561 gacgagctcg ttgaaggcca ggcccgcctt ctcgcgctcg ccgtagccga gccagcagat
     1621 ccgcgccgga agcccctggt acgcgacgcg cttcgccgcc atttcgagcc agcggtggag
     1681 cgcggcgttc tcggggaaga gctcgacgag ggcgcggtcc gtcgcggcga tgtcggccgg
     1741 gtcgcccgag agcgccgccc agcggaacgg ccccttgccg agacagaaga gggggcggat
     1801 gtacttcggg acgaagccct cgatctcgaa ggcgttcgtc acgcccgcct tcacggcctg
     1861 ggcgcggatg ttgttgccgt agtcgaacgt gacggcgccg cggcgctgca gctcgagcat
     1921 gagccgcacg tgctcgccca tcgacgcgac ggcacggcgg ccgtactccc ccgggtcgct
     1981 cttccggagc gcgagggccg cctcgtacga gatcccgtgc gggacgtacc cgttcagctc
     2041 gtcgtgcgcc gaggtctggt cggtcaggag gtcggggacg acgccgcgcg cgacgaggcg
     2101 cgcgagcagg tcgacggcgt tcgcgaggac gccgatcgag atcccctcct tcgcgtcgcg
     2161 cgcggcgagg gcgcggtcga tcgcggagtc gaggtcgtcg atccgttcgt cgcagtagcg
     2221 cgtctcgaga cgccgctgga tccggtgcgc gtcgacctcg gcgacgagcg ccgtgccgcc
     2281 ccccatcgtg acggcgagcg gctgggcccc gcccatccct ccgaggcccg cggtgacggc
     2341 gagcttcccg gcgagcgagc cgtcgggcga gccgaggtcg cggcggcccg cttcggcgaa
     2401 cgtctcgtac gtcccctgga ggatcccctg tgtcccgatg tagatccacg agccggccgt
     2461 catctgaccg tacatcgtca ggccgagcgc ctcgagcctc cggaactcgt cccaggtccc
     2521 ccacttcggg acgaggttcg agttcgcgat gaggacgcgc ggggcgtcgg cgtgggtgcg
     2581 gaagacggcg acgggcttgc ccgactggac gaggagcgtc tcgtcgtcgc cgagcctctc
     2641 gagctcgcgc aggatcgcct cgaggcactc gggatttcgg gcggccttgc cggagccgcc
     2701 gtagacgacg aggtcctcgc ggcgctcggc cacgtccggg tcgaggttgt tcagcagcat
     2761 ccggtagggc gcttccgtca gccaggagcg gcaccggagc gtcgtcccgc gcgggagggc
     2821 cggcggggcg tagcgggcgg gggcggagac ggtgctcatg ccgcggatgt tagtccggaa
     2881 ggcgcggacg ccggcgctcc cccggccccg ggccgaaccg aaacgcgccg catcaacgcg
     2941 tcgtgctact tgacattcat tacgcggcgc gtcaaattgt acgggcgatg acgagccgtc
     3001 acagggagat cgtccgatac cggaaccgga agctctacga gaagagcgag cgccgattcg
     3061 tgacgctcga cgacatcgcc cgggccgtcg ccggaggaga ccgcatccgc gtcgtctccg
     3121 ccgacacggg cgaggacatc accgcgaaga tcctctcgcg cgctctcgcc tccgatcgtg
     3181 tcgcgttccc cgcctcgacg gatgccctcg ccctcctcct ccgcgccggg agcgacgcgg
     3241 ccgagacggt ggccggcgtc gccgagaggg tcggcgcggc gcgcatggcc ggcgccgtcc
     3301 gaaaggcggc gcagccggag cggatcgccg agacgttcgc gcccgtcacg cggagggtcg
     3361 agacggctcg ccacgacgtg gagcggatcg tcgccggcct cgtcggccga ggacggctca
     3421 cctgggagga gggggcccgt ctccgggaag acgtgggaac ggtcttccgg gaaagccttt
     3481 ccgacgtcgt cggtcgtgtc cgggacctcg tccgcagggt cccccccgcg tcctctccgg
     3541 ccatccacgc ggagatccag gagctcgccc gccggctcga ccacctcgag gccctcgccg
     3601 ccgcctcgtt cccgaagggg agctccgccg cgccccgatc cgaatacgcc gcgcgccccg
     3661 cgccggcacg aaagaaggag accagaaagc catgacgagg aagacgaccc gccgagccgc
     3721 tcctgccgcc aagccgctcc ccttcgacgt cccgccgcag atcgaccggg ccctccggac
     3781 cgcggtcgag aaggtcgacg ccgtgaggga cgagacccgc accgtcgcct cgaaggtcga
     3841 gaagacggtc cgggccgccg ccaccagcgc gaagaagacc ggcgagacgc tcgcccgcga
     3901 tccgaagcgc ttcgtggaag gcgtcgtccg cgacgcgaag tccaacctga agaccgtcgg
     3961 ggccgacgtc tcgcgcgagg ccggacgcgt cgccgacgag gtcgcccgcc gcgtctcggc
     4021 cgtcgtcgag cccgccctcg agaaggccct gcaccgcctg aacgtcccga cgcagaagga
     4081 cctgaagtcc ctggtggcga aggtcgacgc gctcggacgc aagatggagc agctgcacgc
     4141 gccggcccgg aagccgcgga gccgaacgcg cgcctgacac cgcggctcct tccccgatcc
     4201 ggatagactg gcgccggcgc ccccggcgcc cgtcttcttt tcaggaggtt ccgtggcggc
     4261 accccggctt cgtctcgtct cctcccgcgg cccgtcgagc ccgcgccccc tctcgagccg
     4321 tcgcgggctc gtctgcgccg gcggcgggat caccggcgcg atctacgaga tcggagtcct
     4381 cgccgccctc gaggaccggc tcgacggctt ctcgctgacg gactgcgacg tcttcgtcgg
     4441 cgtctccgcg ggcgcctatg tgggggctct cgtcgcgaac gggacctccc cctccgtcct
     4501 ctaccgcaac gcgacccacc cgcgcggcga ggggctcgac cggctctcct tctaccgccc
     4561 caacctcggc gagatcgcct cgcgggtcgt gtccgccccg aggatcgtcc tcgaggcgct
     4621 ccgcgacctc cgcgacagcc ccggcgacct cacgctgacc gacctcgtcg cctcgctcgc
     4681 cggcctcctc ccctccggct tcttcctcct cgacggcctc gaggcgtggc tcgccgagtg
     4741 gctctcccac cccgggcgga cgaacgactt ccgcgccctg cgccgcgacc ttcggctcgt
     4801 ggcggtggcc ctcgactcgg gtgagacgcg cgtcttcggc tcctcgcgcg acgaggccgt
     4861 cccgatctcg cgagccgtgg ccgcctcgtg cgccctcccc ggcttcttcc gtcccgtgag
     4921 gatcgacgga gtcgactaca tcgacggcgg tgtgaggaag acggcgcaca tctccctcgc
     4981 cctcaaggag cgctgcggcc tcgtcctctg cgtgaacccg atcgtcccgc tccgctatcc
     5041 gctcgcgccg tccctttccc cccgctaccc ggggcaggga gcgctcgccg gccgcggcct
     5101 cctctccgtc ctcgaccagg tcttccgcct gtcgctccat tcccggctcc gctacggcct
     5161 cgaccgctac cgtcgcgact accccgacgc cgacatcgtc ctcttcgagc cgaaggccga
     5221 agagcttccg cgcctcatgc ggaacatgat gcggacgtcg ggccgcgtgc gaatcgccga
     5281 gtacgcctac cggacgacgc agcagaagct cgacgcggag tacgggcgcc tctcgcgcgt
     5341 cttcgagcgc cacggcctcc ggctgcgtcc cgcaacgtcc gtccgccggg cgctgcgccg
     5401 cgggacggta ccgcggcccg ccgcggcccg ggccccgtct actcgccggt gaagacgttc
     5461 ccgcggcggg cgaggcgcga gtcgatcatc cgctggatcg tcgaccggac gaggtccgtc
     5521 acctcgttca cgatcatcgg acgctcggcg taggccgggt cgaggcccgc gtcgatcggc
     5581 gcgcccacgt cgatccacca cttcgtcggc gcggggacga ggacgagcgg ccccaggagc
     5641 gggaacgtcg gc
//
LOCUS       DAOTEZ010000284         5612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_10332_length_5612_cov_4.566493, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000284 DAOTEZ010000000
VERSION     DAOTEZ010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10332_length_5612_cov_4.566493"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="P5164_13975"
     CDS             complement(<1..1035)
                     /locus_tag="P5164_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRY45048.1"
                     /translation="MSEIAPFPEGFLWGAATSAYQVEGSPLADGAGPSIWHRFARIPG
                     KVRDGDTGDVACDHYNRYAEDVALMKALGLGAYRFSISWSRVLPEGRGRVNEKGLDFY
                     RRLVAALREAGIRPMATLYHWDLPAALDDRGGWTNPDAASWFADYATACYRALDGAVD
                     LWATLNEPWVVADGGYLHGVLAPGIASPWAAPRASKNLLLAHAAGVAAYRAEGKGQVG
                     LVVNLEPKYPASERPEDLAAVARADAYMNRQYLDPALLGKVPEGLAGIFGEAWEGPTG
                     AELAAMKAPIDFLGVNYYTRAVVYDDPRSFPVMEGRAFQRNATHTETGWEVFPDGLYD
                     VLTWVRRRYGN"
     gene            1114..1998
                     /locus_tag="P5164_13980"
     CDS             1114..1998
                     /locus_tag="P5164_13980"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009787076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY45049.1"
                     /translation="MSDVSLLGTGLLGAPIVRRLAATGLSVTVWNRTAAKAEALGGEG
                     LAVAPTAASAFEASPVAILMLADAPAIRAVLAAPGVCDALAGKSVVQMGTIGPDESRA
                     FAAEVAAAGGEWVEAPVLGSIPQADSGTLQVMVGGTEEQLARLEPLFSRLGHDVRLVG
                     GVGRAAALKLALNQLIGSLTTAFATSLAFVRASGVPTELFLEVLRPSALYAPTFDKKL
                     GRMLEAEYGNPTFPLRLLGKDLRLFERAAREAGLEPIVVDAVLRTVEAAEARGHAEDD
                     YAALAEGIAELPPVLPRG"
     gene            2010..3491
                     /locus_tag="P5164_13985"
     CDS             2010..3491
                     /locus_tag="P5164_13985"
                     /inference="COORDINATES: protein motif:HMM:NF006088.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HRY45050.1"
                     /translation="MSGAEHGSGGADVPLVVDLDGTLLRTDLLHESAVRALVRAPRAA
                     LSAVPALLRGGRAALKRRLATVAPIDAETLPLREEVVAHLGEEKARGRRLVLATAADE
                     LLARPVAERVGLFDELLASDGRRNLGGEAKRAELVARFGEKGFDYVADRRADLPVFRS
                     ARKAVLVGSGVRLRAEVEAAGTPVTALLPDGVPRARTLLSAIRARQWVKNLLVFVPLV
                     TAHVFTPAAFGAAGVAFLALSLLASAVYLLNDLADLPADRRHPSKRSRPIAAGRLPIR
                     TAVAFVPVLLAGAAALTFALPRGARLLLLAYLGATTLYTLLLKRKVLVDVFTLAFLYT
                     LRVLLGGAATGVLVSPWLLAFSVFFFLSLAFVKRASELVSVRERGLDSAAGRDWRATD
                     GFTVPVLGAASGYLSAVVLAIYVHSDVTKRLYGHPGWLWLLVPILLYWVSRLWVLVGR
                     GEMDDDPVLFAARDAVTWGLAAATAAALLLAALGPSGVPGLME"
     gene            3615..>5612
                     /locus_tag="P5164_13990"
     CDS             3615..>5612
                     /locus_tag="P5164_13990"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRY45051.1"
                     /translation="MVRTRTAPGVVLCLALTLLPHGAFAQSAATTGAIEGTVADESGG
                     VLPGARVVLRNTATNYETTVATAANGRFRGILLPLGPYRVTVTLPGFAKVVREGLNVS
                     VGQSVNVAVTMRLAAKAEEVLVTAENPVVETTRPEGTTRIDTLAVRSLPNNGRNFMDF
                     TKLTPGVSIVQGPDGDELSINGQKGIQNNVSVDGADFNNPFFGEQRGGQRPAFTFNLD
                     AVQEVVVVADGANAEYGRSSSGFVNVVTKSGTNQLAGTVHVFYKDDSLASAAKRPDGS
                     SADKYDSSQVQGGFTLGGPVVTDRVFFFAAVDAQGGSSTKQTDPNRIEQRVVDFFASL
                     GSPDENGSIERGNDAFVALAKVDWLLGSKHTVTLRGTYTKSSQENGTFDVDSWGRSAN
                     ATEDNWSRSLTGTAISNFGSTLNEFRFQLAREDRPRPYDGPNIAGQNRPLPDTAFDFG
                     KGYRFGMPFFIPVDYYDTRIQLNDNVTYLTGAHELKAGVEFNRVNAAQVFRGFANGRY
                     IFSSTDGFLNYARNPSYVECSDGSSSPNGTCPVGTTVTGPVLLFLQQAGVGGLTAEEA
                     GTQAIRQDEPAVFVQDKWQPTANLTVQLGLRWEAQIQPDPITPAEDVFYAGFIGKTSM
                     GQEFPSDGNIPSDKSMWQPRIGITWDPTNDGRTVVRANAGLY"
BASE COUNT          756 a         2034 c         2011 g          811 t
ORIGIN      
        1 gttcccgtag cggcgccgga cccacgtcag gacgtcgtag agcccgtcgg gaaagacctc
       61 ccagccggtc tccgtgtgcg tcgcgttccg ctggaacgcg cgcccctcca tgacggggaa
      121 gctccgcgga tcgtcgtaga cgacggcgcg ggtgtagtag ttgacgccga ggaagtcgat
      181 cggcgccttc atcgcggcga gctcggcccc ggtcggcccc tcccacgcct cgccgaagat
      241 ccccgcgagc ccctcgggga ccttcccgag gagcgcgggg tcgaggtact ggcggttcat
      301 gtaggcgtcg gcgcgggcga cggcggcgag gtcctcgggg cgttcggagg ccgggtactt
      361 cggctcgagg ttgacgacga ggccgacctg ccccttccct tcggcccggt acgcggcgac
      421 tccggccgca tgggcgagga ggaggttctt cgaggcccgc ggcgcggccc aggggctcgc
      481 gatcccgggg gcgaggacgc cgtggagata gccgccgtcc gcgacgaccc acggctcgtt
      541 gagggtggcc cagaggtcga cggcgccgtc gagggcgcga tagcaggccg tcgcgtagtc
      601 ggcgaaccag gaagcggcgt cggggttcgt ccagccgccg cggtcgtcga gggcggcggg
      661 gaggtcccag tggtagagcg tcgccatcgg gcggatcccc gcctcgcgaa gcgccgcgac
      721 gaggcggcgg tagaagtcga gccccttctc gttcacgcgc ccgcgcccct cggggaggac
      781 gcggctccag gagatcgaga agcggtaggc gccgaggccg agcgccttca tgagcgcgac
      841 gtcttcagcg taccggttgt agtggtcgca ggcgacgtcg ccggtgtctc cgtcccggac
      901 cttgccgggg atccgggcga agcggtgcca gatgctgggg ccggcgccgt cggcgagggg
      961 cgacccctcg acctggtagg cggacgtggc ggctccccag aggaagcctt ccgggaacgg
     1021 ggcgatctcg ctcatcgggt ctcctcgcgc gtgggggcgc acggtctcgc aggggcggtt
     1081 cccgattatc cgcgcgcgtc ggagaatcgc cagatgagcg acgtctccct cctcggcacg
     1141 ggactcctcg gcgcgccgat cgtgcgccgg ctcgcggcga ccggcctctc cgtcacggtc
     1201 tggaaccgga cggcggcgaa ggcggaggcg ctgggcgggg aggggctcgc cgtggcgccg
     1261 acagcggcgt ccgcgttcga ggcctcgccc gtcgcgatcc tgatgctcgc ggacgccccg
     1321 gcgatccggg ccgtcctcgc cgcaccgggc gtctgcgacg cgctcgccgg gaagtccgtc
     1381 gtccagatgg ggacgatcgg tcccgacgaa agccgggcct tcgcggcgga ggtcgcggcc
     1441 gccgggggcg agtgggtcga ggcgccggtc ctcgggagca tcccgcaggc ggattcgggg
     1501 acgctccagg tgatggtcgg cgggaccgag gagcagctcg cgcggctcga gccgctcttc
     1561 tcccggcttg gccacgacgt ccgcctcgtc ggaggggtcg gccgggcggc ggcgctgaag
     1621 ctcgctctga accagctcat cggctcgctg acgacggctt ttgcgacgag cctcgcgttc
     1681 gtccgcgcgt ccggcgtccc gaccgagctg ttcctggagg tcctccggcc gagcgccctc
     1741 tacgccccga cgttcgacaa gaagctcgga cggatgctcg aggccgagta cggaaacccc
     1801 acgttcccgc tccgcctgct cgggaaggac cttcgcctct tcgagcgcgc cgcacgggag
     1861 gcgggcctcg agccgatcgt cgtcgatgcc gtcctccgga ccgtcgaagc ggccgaggcg
     1921 cggggccacg cggaggacga ctacgccgcg ctcgccgagg ggatcgcgga gcttcccccc
     1981 gtccttccgc gcgggtaacc tcgggggacg tgagcggcgc cgaacacgga agcgggggag
     2041 cggacgtgcc gctcgtcgtc gacctggacg ggacccttct tcggacggac ctcctccacg
     2101 aatccgcggt ccgcgcactc gtccgcgcgc cgcgcgccgc gttgtcagcc gttcccgcgc
     2161 ttctccgagg cggccgcgcc gccctgaagc ggcggctcgc gacggtcgca ccgatcgatg
     2221 cggagacgct ccccctccga gaggaggtcg tggcgcatct cggggaagag aaggcccgcg
     2281 ggcggcggct cgtcctcgcc accgccgccg acgagctcct cgcgcggccc gtcgccgaga
     2341 gggtcggtct cttcgacgag ctcctcgcga gcgatgggcg gcgaaacctc ggcggcgagg
     2401 cgaagcgggc cgagctcgtc gcccgcttcg gcgagaaggg cttcgactac gtcgccgacc
     2461 gccgggccga cctgccggtc ttccggagcg cccggaaggc ggtgctcgtc ggttccggcg
     2521 tccggctgcg ggcggaggtc gaggcggcgg gaacgccggt caccgctctc ctgccggacg
     2581 gcgtgccgag ggcccgcacg ctcctctccg cgatccgcgc gcggcagtgg gtcaagaacc
     2641 tcctcgtctt cgtccccctc gtcaccgcgc acgtcttcac gccggcggcg ttcggcgcgg
     2701 cgggggtcgc gttcctcgcc ctctcgctcc tcgcttccgc cgtctacctc ctgaacgacc
     2761 tcgccgacct gccggccgat cggcggcacc cgtcgaagcg ttcccgcccg atcgccgccg
     2821 gccggctccc catccggacg gccgtcgctt tcgtaccggt gctcctcgcg ggggcggcgg
     2881 ccctgacgtt cgcgctcccg cgaggcgcgc ggctcctcct cctggcctac ctcggcgcga
     2941 cgacgctcta cacgctgctc ctgaagagga aggtcctcgt cgacgtcttc acgctcgcgt
     3001 tcctctacac gctccgggtc ctcctcggcg gcgcggcgac gggcgtcctc gtctcgccgt
     3061 ggctcctcgc gttctccgtc ttcttcttcc tctccctcgc cttcgtgaag cgggcttccg
     3121 agctcgtctc ggtgcgggag cgcggcctcg actcggcggc cggccgggac tggcgggcga
     3181 cggacggctt cacggtcccg gtcctgggcg cggcgagcgg ctacctgtcg gcggtcgtcc
     3241 tcgcgatcta cgtccacagc gacgtcacga aacgcctcta cgggcacccg ggctggctgt
     3301 ggctcctcgt gccgatcctc ctctactggg tgagccggct ctgggtcctg gtggggcgag
     3361 gcgagatgga cgacgacccg gtcctcttcg cggcccggga cgccgtcacg tgggggctgg
     3421 cggcggcgac ggccgcggcg ctcctcctcg ccgcgctcgg tcccagcggc gtccccggcc
     3481 tgatggagta ggccgggccg cgacgcacgg ggccgcggat ctccctttga cccgtgaccg
     3541 cggacgggaa tagtgtcccc ggcaagctca gggatgccga gatgccgctc gaaggcgtcg
     3601 gaaggagacg gtcgatggtg cgcacgcgaa ccgccccagg ggttgtcctc tgcctcgccc
     3661 tgacgttgct cccccacggg gccttcgccc agtcggccgc gacgacgggc gcgatcgagg
     3721 ggaccgtcgc ggacgagtcc ggcggggtcc tcccgggtgc ccgcgtcgtc ctccggaaca
     3781 cggcgacgaa ctacgagacg acggtcgcga ccgccgcgaa cggacggttc cgcgggatcc
     3841 tcctgccgct ggggccctac cgggtgaccg tcactctccc gggcttcgcg aaggtcgtcc
     3901 gcgaggggct gaacgtctcg gtcggccagt cggtgaacgt cgcggtcacg atgaggctcg
     3961 ccgccaaggc cgaagaagtc ctcgtcaccg ccgagaaccc ggtcgtcgag acgactcggc
     4021 ccgaggggac gacccggatc gacacgctcg ccgtccggtc gctgccgaac aacggccgga
     4081 acttcatgga cttcaccaag ctcacgccgg gcgtctcgat cgtccagggc cccgacggcg
     4141 acgagctctc gatcaacggc cagaagggga tccagaacaa cgtctccgtc gacggcgccg
     4201 acttcaacaa cccgttcttc ggagagcagc ggggcggcca gcggcccgcc ttcacgttca
     4261 acctcgacgc ggtgcaggag gtcgtcgtcg tcgccgacgg ggcgaacgcc gagtacgggc
     4321 gctcctcctc gggcttcgtg aacgtcgtca cgaagtcggg gacgaaccag ctcgccggga
     4381 cggtccacgt cttctacaag gacgactccc tcgcctcggc cgccaagcgc ccggacgggt
     4441 cgtcggccga caagtacgac tcgagccagg tccagggggg cttcacgctc ggcgggccgg
     4501 tcgtcacgga ccgcgtcttc ttcttcgcgg ccgtcgacgc gcagggcggc tcctcgacga
     4561 agcagacgga cccgaaccgg atcgagcagc gcgtcgtcga cttcttcgcg agcctcggca
     4621 gcccggacga gaacggctcg atcgagcggg gcaacgacgc gttcgtcgcg ctcgccaagg
     4681 tcgactggct cctcggctcg aagcacacgg tgacgctccg cggcacctac acgaagtcga
     4741 gccaggagaa cggcaccttc gacgtcgact cctggggccg gagcgcgaac gcgaccgagg
     4801 acaactggtc gcgctccctc accggcaccg cgatctcgaa cttcggctcg acgctgaacg
     4861 agttccgctt ccagctcgcc cgggaggacc ggccgcggcc gtatgacggc ccgaacatcg
     4921 ccggccagaa ccggcccctc cccgacacgg cgttcgactt cgggaagggc taccggttcg
     4981 ggatgccgtt cttcatcccg gtcgactact acgacacgcg catccagctg aacgacaacg
     5041 tgacgtacct gacgggagcg cacgagctga aggcgggcgt cgagttcaac cgcgtgaacg
     5101 cggcccaggt cttccgcggc ttcgcgaacg gccggtacat cttcagctcg acggacggct
     5161 tcctgaacta cgcccggaac ccgagctacg tcgagtgctc cgacggctcc tcgagcccga
     5221 acgggacctg ccccgtcggg acgacggtca ccgggcccgt cctcctcttc ctccagcagg
     5281 cgggcgtcgg cgggctgacg gccgaggagg ccgggacgca ggcgatccgg caggacgagc
     5341 cggcggtctt cgtccaggac aagtggcagc cgaccgcgaa cctgaccgtc cagctcggcc
     5401 tccgctggga ggcgcagatc cagcccgacc cgatcacccc ggcggaagac gtcttttacg
     5461 ccggcttcat cgggaagacg tcgatgggcc aggagttccc gtcggacggg aacatcccgt
     5521 cggacaagag catgtggcag cctcgcatcg gcatcacgtg ggacccgacg aacgacggcc
     5581 ggaccgtcgt gagggcgaac gcgggcctct ac
//
LOCUS       DAOTEZ010000285         5588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_10396_length_5588_cov_7.572022, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000285 DAOTEZ010000000
VERSION     DAOTEZ010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10396_length_5588_cov_7.572022"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="P5164_13995"
     CDS             complement(<1..311)
                     /locus_tag="P5164_13995"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRY45052.1"
                     /translation="MSAEGSRPPERGGFQPPEAPTFRCPSCGAPAAKDARACVHCGSL
                     LATKRCVTCFALSPREADRCVKCGALLPAPAFVAEGSGRCPDCKLDLVAHAFGAVGYA
                     E"
     gene            complement(283..762)
                     /gene="tadA"
                     /locus_tag="P5164_14000"
     CDS             complement(283..762)
                     /gene="tadA"
                     /locus_tag="P5164_14000"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HRY45053.1"
                     /translation="MSRTSHADDERFMREALRAARRAPAHRDVPIGAVVVRDGLVVAR
                     ARNRREVDHDPTAHAEVLVLRRAARRLGSWRLDGCTLYVTLEPCAMCAGAIVLSRLPR
                     LVFGAADPKAGFVGSLGDLCRDARLNHRVHVEDGVLGAECGTILKEFFRERRRKPAP"
     gene            complement(829..2256)
                     /locus_tag="P5164_14005"
     CDS             complement(829..2256)
                     /locus_tag="P5164_14005"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY45054.1"
                     /translation="MTPGPPEGGRLRQVLAAVMAGAILGPIDGSIVNVVLPTLVDAFG
                     ASLASVQWVPMAYLLASGGLVLVFGRLGDLRGHREVFLGGLLAFVAASALCAAAPTIE
                     ALVAFRVLQGVASAAMTSVPLAILTATFPPAERGRAIGLFAASISVGLAIGPSLGGFL
                     TATFGWRAVFLVNLPIGLAAWAYARRVLPEVPIRPGRLDLPGAAAALVALGSALLWVN
                     LAQRPGPAPHGLLLLALLAGAAFLLRERRAAEPMVALSLFRSGAVSFGASASVLSFMA
                     QFVVVFLTPFLLVRVLGAGPGRVGLVLTASPLAVLAVAPFAGALSDRIGSVGPAVAGS
                     LLSATACALLAAMPPDAGPLAVAVRLALFGVGTGLFQSPNNSSVMGSAPREHLGVVGS
                     LLGTARTLGMVLGIAAAGAVLYAFVPPSALGADGTTPPLAAELASGVRRAYAAGAGFA
                     LLSAALAAAPALARRAGRTGGPAIP"
     gene            complement(2253..4757)
                     /gene="ptsP"
                     /locus_tag="P5164_14010"
     CDS             complement(2253..4757)
                     /gene="ptsP"
                     /locus_tag="P5164_14010"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HRY45055.1"
                     /translation="MTVEIAFAFPLPGGLHARPAARLQEEVARFRSVVTFLNRANGTS
                     ASARSVLALVATRTALGEPCVLHVQGEDEAAAGAALRKFVKRELPGLDDGPPARAGTG
                     APLPRVLARAEARVHRGAPASGGIFRGRAVVVGSRRAAVDEERTRTEAEELARLEAAF
                     EKAAAAIRVRLAAAEYETERAVLEAHLSIAEDPGLRARAAAAVVEGAAAPSAVFAAAG
                     HFAEIFRASGSNLLAERALDLHDVAEAVAHVLSGDGVPPPAVELGEAAVVVAARLGPG
                     QLLALPRARVRGLVLAEGGTLSHTVVLARAFGVPCVTGVDGIERALATGDDVVVDGER
                     GLVVVAPKGPVLRFYEAQSELLSTAARRLERFRTARAVTADGRRIEVGANVGTVEEAA
                     SAFENGAEGIGLFRTELMFLDRDEPPTEEEQLAIFAETARLAEGRPVIVRTLDVGADK
                     PLAWLRLPSERNPALGYRAIRMYAEHAELAERQLRAILRAGAVGPLKVMFPMVGTPEE
                     ARELRALVEKVKGLLSAEGVAHDPDVPVGVMLEVPSAILSLRAIAREVDFFSVGSNDL
                     AQYLFAVDREDTRLAHLGSPYHPAFLRLLSEAVEGAHAAGKWIGLCGELGGRPLAAPL
                     LLGLGFDEVSVSPPRVLRAKAAFRRVRSAGARSLVAEALDLSTAGEVEALLVDRAPAA
                     RTLVDAGSVRAASGSGSRDEAIRELADLLQLTGRADVADRVEDAIWAREEVETSLLAR
                     GVAFPRCVSPHVRADSIAAVRLSPPLPWGDGGEKVELAILLALRPGAGEAKHRRTAEE
                     LRRSLGEAAVRRRLLEAPDDASLAALLAEAAGRAAP"
     gene            complement(4935..5408)
                     /locus_tag="P5164_14015"
     CDS             complement(4935..5408)
                     /locus_tag="P5164_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45056.1"
                     /translation="MHIDAVLRKLGELERSLADLYGWWSEVFAADEEAAFVFFKMKGE
                     EKAHAGLVDYQRKLVQNSPALSVDVSFDLTEVEAALARAKSLRGAAVPPTVAEALAEA
                     LKIERSAAESHCRNALKLASPGIARLLDALGGEDKLHVARLAELAERRGITVAPA"
BASE COUNT          772 a         2116 c         2143 g          557 t
ORIGIN      
        1 cactcggcgt agccgaccgc gccgaaggcg tgggccacga ggtcgagctt gcagtcgggg
       61 cagcgcccgg acccttcggc cacgaacgcg ggcgccggaa ggagcgcgcc gcacttcacg
      121 caccggtcgg cctcgcgcgg cgagagggcg aagcaggtga cgcagcgctt cgtcgccagg
      181 agcgagccgc agtggacgca ggcgcgcgcg tccttcgccg cgggggcgcc gcaggacggg
      241 cagcggaacg tcggggcttc ggggggctgg aagcccccgc gttcaggggg ccggcttcct
      301 tctgcgctca cggaagaact ccttcaggat ggtcccgcac tccgcgccga ggacgccgtc
      361 ctcgacgtgg acccggtggt tgagccgcgc gtcgcggcag aggtcgccga gcgagccgac
      421 gaagcccgcc ttcgggtcgg cggcgccgaa gacgagccgc ggcaggcgcg aaaggacgat
      481 cgcgccggcg cacatcgcgc acggctcgag cgtgacgtag agcgtgcagc cgtccagccg
      541 ccacgagccg agccgccgcg cggcgcgccg gaggacgagg acctcggcgt gcgccgtcgg
      601 gtcgtggtcg acctcgcgcc ggttccgggc gcgggcgacg acgaggccgt ccctcacgac
      661 gacggccccg atcgggacgt cgcggtgcgc cggggcgcgg cgggccgcgc gcagcgcctc
      721 gcgcatgaac cgctcgtcgt cggcgtggct cgtccggctc acgggtcgga ctccggcccc
      781 cgaacgggag cggcggcgcg acggggcgga gggttcacgc ccccaaggtt acgggatggc
      841 gggcccgccc gtccgcccgg ccctccgggc gagggccggc gccgccgcca gggccgccga
      901 gagaagggcg aagcccgcgc cggccgcgta cgcccgccgc acgccggagg cgagctccgc
      961 ggcgaggggc ggcgtcgtgc cgtcggcgcc cagggccgac ggcggaacga acgcgtagag
     1021 caccgcgccc gccgcggcga tgccgaggac catcccgaga gtccgcgccg tcccgagaag
     1081 gctcccgacg acgccgaggt gctcgcgcgg cgcgctcccc atgaccgagc tgttgttggg
     1141 cgactggaag aggcccgtcc cgacaccgaa gagggcgagc cggacggcga cggcgagcgg
     1201 accggcgtcc ggcggcatcg ccgcgaggag ggcgcacgcc gtcgccgaga ggagcgagcc
     1261 cgccaccgcc gggccgacgg agccgatccg gtcggagagc gccccggcga agggggccac
     1321 cgcgaggacc gcgagcgggg aggccgtcag gacgaggccg acgcgcccgg ggccggcgcc
     1381 gaggacccgg acgaggagga agggcgtcag gaagacgacg acgaactgcg ccatgaagct
     1441 caggaccgag gccgacgcgc cgaacgagac ggcgccgctc cggaagaggg agagcgccac
     1501 catcggctcc gcggcgcgcc gctcccggag gaggaacgcc gccccggcga ggagcgcgag
     1561 gaggaggagc ccgtgcggcg ccggaccggg gcgctgcgcg aggttcaccc agaggagagc
     1621 cgatccgagg gcgacgagcg ccgccgccgc gccggggaga tcgagccgcc ccgggcggat
     1681 cggaacctcc ggcagcacac ggcgcgcgta cgcccaggcg gcgagcccga tcgggaggtt
     1741 cacgaggaaa acggcgcgcc agccgaaggt ggccgtcagg aacccgccca ggctcggacc
     1801 gatcgcgagg ccgacggaga tgctcgccgc gaagagaccg atcgcccggc ccctctcggc
     1861 cggcgggaac gtcgccgtca ggatcgcgag cggcaccgac gtcatcgccg ccgaagcgac
     1921 cccctggagg acgcggaagg ccacgagcgc ctcgatcgtc ggcgcggccg cgcagagcgc
     1981 cgaggcggcg acgaacgcga ggaggcctcc gaggaagacc tcgcggtgcc cgcgcaggtc
     2041 gccgagccgg ccgaagacga ggacgaggcc gccgctcgcg aggaggtagg ccatcgggac
     2101 ccactggacc gaggcgaggc tcgccccgaa agcgtcgacg agcgtcggca ggacgacgtt
     2161 gacgatgctc ccgtcgatcg gcccgaggat cgccccggcc atcaccgcgg cgaggacctg
     2221 ccgcaggcgg ccgccctccg gcggcccggg cgtcacggcg ccgcgcgccc cgccgcctcc
     2281 gcgaggagtg cggccagcga ggcgtcgtcg ggggcctcga ggaggcggcg ccggacggcg
     2341 gcctcgccga ggctccgccg cagctcctcg gccgtcctgc ggtgcttcgc ctcccccgcc
     2401 ccgggccgca gcgcgaggag gatcgcgagc tcgactttct cgccgccgtc tccccagggg
     2461 agcggcggcg agaggcggac ggcggcgatc gagtcggccc gcacgtgcgg cgagacgcag
     2521 cgcgggaacg ccacgccgcg ggcgaggagc gacgtctcga cctcctcgcg cgcccagatc
     2581 gcgtcctcca cccggtcggc cacgtcggcc cgaccggtga gctggaggag atcggcgagc
     2641 tcccggatcg cctcgtcgcg gctgccgctc cccgaggcgg cgcgcaccga cccggcgtcg
     2701 acgagggtgc gcgccgcggg cgcgcgatcg acgaggagcg cctccacctc tcccgcggtc
     2761 gagaggtcga gcgcctcggc gacgaggctg cgcgccccgg ccgaacggac cctccggaac
     2821 gccgccttcg cccggaggac gcgcggcggc gagacgctca cctcgtcgaa gccgaggccg
     2881 aggaggagcg gcgccgcgag cgggcggccg ccgagctcgc cgcagaggcc gatccacttc
     2941 ccggcggcgt gcgccccttc caccgcctcg gagaggagcc ggaggaacgc cgggtggtac
     3001 gggctcccga ggtgggcgag gcgcgtgtcc tcgcggtcga cggcgaagag gtactgcgcg
     3061 aggtcgttcg agccgacgct gaagaagtcg acctcccgcg cgatcgcccg gagcgagagg
     3121 atcgccgacg gcacctcgag catcacgccg accgggacgt ccgggtcgtg ggcgaccccc
     3181 tcggccgaga ggagcccctt caccttctcg acgagggcgc ggagctcccg cgcctcctcc
     3241 ggcgtcccga ccatcgggaa catcaccttc agcggcccga cggccccggc gcggaggatc
     3301 gcccggagct ggcgctcggc cagctccgcg tgctcggcgt acatccggat cgcccggtag
     3361 ccgagcgccg ggttccgctc cgacggcaga cggagccagg cgagcggctt gtccgccccg
     3421 acgtcgagcg tcctcacgat gacgggacgg ccttcggcga ggcgggcggt ctccgcgaag
     3481 atcgcgagct gctcctcctc ggtcggcggc tcgtcgcggt cgaggaacat cagctccgtc
     3541 cggaagaggc cgatcccctc cgccccgttc tcgaacgcgg aggcggcctc ctcgacggtc
     3601 ccgacgttcg ccccgacctc gatcctccgc ccgtccgccg tgacggcgcg cgccgtccgg
     3661 aaccgctcga gccgccgcgc ggccgtcgaa aggagctcgc tctgcgcctc gtagaagcgg
     3721 agcacgggcc ccttcggggc gacgacgacg agcccccgct ccccgtcgac gacgacgtcg
     3781 tcgcccgtcg cgagcgcccg ctcgatcccg tccacgcccg tcacgcacgg gacgccgaac
     3841 gcgcgggcga ggacgacagt gtgcgagagc gtcccccctt ccgcgaggac gaggccgcga
     3901 acgcgcgccc gcggcagcgc gaggagctgc cccgggccga ggcgcgccgc gacgacgacc
     3961 gcggcctccc ccagctccac ggccggcggc gggacgccgt cgccggagag gacgtgggcg
     4021 acggcctcgg cgacgtcgtg caggtcgagc gcccgctcgg cgaggaggtt gctcccgctg
     4081 gcgcggaaga tctcggcgaa gtggcccgcc gcggcgaaga ccgcggaggg cgcggcggcc
     4141 ccctcgacga cggcggccgc ggcgcgagcc cgcagcccgg ggtcctcggc gatcgagagg
     4201 tgggcctcga ggacggccct ctccgtctcg tactccgccg cggcgaggcg gacccggatc
     4261 gcggccgccg ctttctcgaa cgccgcctcg aggcgggcga gctcctccgc ctccgtccgg
     4321 gtccgctcct cgtcgaccgc ggcgcggcgc gagccgacga cgaccgcgcg cccgcggaag
     4381 atcccgccgc tcgccggcgc cccgcggtgg acgcgcgcct cggcccgcgc gaggacgcgc
     4441 gggagcggcg cgcccgtccc ggcccgcgcc ggaggcccgt cgtcgaggcc cgggagctcc
     4501 ctcttcacga acttccggag cgcggccccg gccgcggcct cgtcctctcc ctgcacgtgc
     4561 aggacgcacg gctcgccgag cgcggtccgc gtcgccacga gggcgaggac gctccgcgcg
     4621 ctcgcggacg tgccgttcgc gcggttcagg aaggtgacga ccgagcggaa gcgcgccacc
     4681 tcctcctgaa ggcgtgccgc cgggcgcgcg tgcaggccgc cggggagcgg gaaggcgaag
     4741 gcgatctcga ccgtcacggc gaccgccgcc cgcgccccgg acctgatcgc ctccggccga
     4801 cgaggcgtcc cgtcattcgc tccccctgca tcggcgttcc ggatttcggt ccgtcagtct
     4861 agcaagcggg tccaagcccc gaacgccgcg acgcccgccg cgcggggccc cggcttcccc
     4921 ggtcggcggc ggcgtcaggc cggcgcgacc gtgatacccc gccgctcggc cagctccgcg
     4981 aggcgggcca cgtgcagctt gtcctcgccg ccgagggcgt cgaggaggcg agcgatcccg
     5041 ggactcgcca gcttcagcgc gttccggcag tggctctcgg cggcggagcg ctcgatcttg
     5101 agcgcctcgg cgagagcctc ggcgaccgtc ggcgggaccg cagcgccccg gagcgacttc
     5161 gcccgcgcca gcgccgcctc cacctccgtc aggtcgaacg agacgtcgac ggagagcgcc
     5221 gggctgttct ggacgagctt gcgctggtag tcgacgaggc cggcatgggc cttctcctcg
     5281 cccttcatct tgaagaagac gaaggcggcc tcctcgtcgg ccgcgaagac ctcggaccac
     5341 catccgtaga ggtccgcgag cgaccgctcg agctcgccga gcttccgaag gaccgcgtcg
     5401 atgtgcatgg gagcctcctc gccgcagggg cgagcgaggg agtctacggc gccgggatgg
     5461 cgcgacgaaa cgaaaacccc gggggtcgcc cggggtctct tcgcaggaag ctcttcgtct
     5521 tcagggataa ggggtggtgc gcccaggagg actcgaaccc ccaacctttt gatccgtagt
     5581 caaacgct
//
LOCUS       DAOTEZ010000286         5570 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_10450_length_5570_cov_6.727833, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000286 DAOTEZ010000000
VERSION     DAOTEZ010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10450_length_5570_cov_6.727833"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..2972)
                     /locus_tag="P5164_14020"
     CDS             complement(60..2972)
                     /locus_tag="P5164_14020"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRY45057.1"
                     /translation="MKPLLTVAVAAFLALAAASPATAAPPFPEEWLRARPDARGPLEK
                     ADPRLLAPAAAGEEDLEVLATLRLPAEALALGAGSPRAGARTALQARLAGELETDYAP
                     FGVRVEQAFAYVPVLVLRAPRKAVAALAGDPRIERVRPNGRVRALDAEGEGLMRVPEL
                     RAAGVDGAGVTIAVLDSGIDYTHPELPLGTKVVNLYDAIDHDGDARDGYGHGTPVAGI
                     IAGLTTGVARGAKVAAVRVLDTRGEGSDAQVLEGVDAVLASVAEGNPHAIRVVNLSLG
                     GYFDDAAPPEPGRCDLLAPDYKAAFDLLAQAGILVVAASGNGGCTGGIVLPACVSNAL
                     AVGAVYDAAVSPQAFGRGHCLPSGCSDSAPAADAIACYSDSGDRLDVLAPAHCATAPR
                     NGGGSYSCFGGTSAAAPYAAGVAALLFGAGPDRRPTEVKAALRETGKAILDPRNGVTR
                     NRIDAPAALAALRSAPAGAARAELLVPVVLDVVGVGEARFRSELTLTNRGTTAARLEA
                     TYTPATGMFPGAAGGGTVADALAPGEQRVIPDAIAWLRAAKGLPIPLGPGQAGSLRLV
                     FTGLSPAAETVSARVRTTAAACGGAAGLSYAAWKLGEGPAAPLRLFGLRSGPAERSNL
                     AVVNMGTEGTVTPRIILRSGDGASVYEVPAALLPPLAPGEWRQVNDADLLRAAGFAEA
                     TALVSRAAGTAPLGAYAVFNDNLSHDGSFVPAVPDAFAGELVLPVVVETPAFESELVL
                     FNPEAAPAVVSLAYTESLATPKGRVGAVSIELAPFEQRILPRALDTLRDLGLPLGPRG
                     AASYAGTLQALFASPTTTLVPGLAGAKTSAAGTGACAGNGSFGLFYTAVPTASAASVE
                     AVVDGLRQTATTRSNLALFNPSATVPVTVAYDVHDGATGAKVSSSAPVDLPPLGWTQV
                     DRVLLGAGVTEGFVRVRRTSSAGVFGAYGVLNDGAAPGERTGDGSYVAGEAYDPPD"
     gene            3302..>5570
                     /gene="nifJ"
                     /locus_tag="P5164_14025"
     CDS             3302..>5570
                     /gene="nifJ"
                     /locus_tag="P5164_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HRY45058.1"
                     /translation="MTTRPMVTLDGNEAVASVAHRVSEVIAIYPITPSSTMGEFSDEW
                     SAQGRKNVWGTVPLVSEMQSEGGAAGAVHGALQAGALTTTFTASQGLLLMIPNMYKIA
                     GELTPFVMHVSARCVATHALSIFGDHSDVMACRQTGFAMLASGSVQEAHDLAAVAHRA
                     TLMSKLPFLHFFDGFRTSSEVAKIERLDDEDLLAMVSEDLVSAQRKRALTPDAPVLRG
                     SAQNPDCFFQAREACNGYYDAAPGLVQEAMDAFAKLTGRAYRLFDYVGHAEAERVIVI
                     MGSGAEVTHETVDHLVARGEKVGVLKVRLYRPFDASAFLGALPKSVKAIAVLDRTKEP
                     GAVGEPLLQDVLTVLRDAEETGTSPFAASPKVVGGRYGLASKEFTPAMVKGVFDELAQ
                     AKPRRRFTVGIVDDVTHLSLKWDAEFDTEPAEVKRAVFFGLGADGTVGANKNSIKIIG
                     EETDNYAQGYFVYDSKKSGGITISHLRFGPKPIRSSYLVSTAGFVACHQFGFLEKYDV
                     LEYAAPGAVFLLNSPYGPDEVWDKLPVEVQAQILEKKLRFFVIDGYEVGAKTGMGRRI
                     NTVMQTCFFAISGVLPREEAIAQIKKSIEKTYGKKGAELVRMNFEAVDQTLENLFEVK
                     VPSAATSKLKRPPIVPQEAPDFVQKITAIMLANKGDLLPVSAFPVDGTFATATARWEK
                     RNIAIEIPVWDEKLCIQCNKCAMVCPHAAIRAKVFDPAVLEKAPGTYKSVDYRAPEFK
                     GMKYTIQVAPEDCTGC"
BASE COUNT          822 a         1985 c         2025 g          738 t
ORIGIN      
        1 gtgcacgttc ttcgcggcca tggctcctcc ctgtgcggga ggaatgctac cgccgagtct
       61 cagtcgggcg ggtcgtacgc ctcccccgcg acgtagctcc cgtcgccggt cctctctcca
      121 ggggcggcgc cgtcgttcag gacgccgtag gcgccgaaaa cgccggcgga ggacgtccga
      181 cggactcgga cgaacccctc ggtgacgccg gcgccgagga ggacgcgatc gacctgcgtc
      241 catccgagcg gcgggaggtc gacaggcgcg gaagaagaga ccttcgcccc ggtcgccccg
      301 tcgtggacgt cgtacgccac ggtgaccggc accgtcgccg acgggttgaa gagcgcgagg
      361 ttcgagcggg tcgtcgcggt ctggcggagg ccgtcgacga ccgcctcgac cgaggccgcc
      421 gaggcggtcg gcaccgcggt gtagaagagg ccgaagctcc cgtttccggc gcacgctccc
      481 gttccggcgg cggaggtctt cgctcccgcg aggccgggaa ccagcgtcgt ggtcggggac
      541 gcgaagaggg cctggagcgt cccggcgtac gacgcggcgc cgcggggccc gagcgggagg
      601 ccgaggtccc ggagcgtgtc gagggcccgc gggaggattc gctgctcgaa cggcgcgagc
      661 tcgatcgaga ctgctccgac ccgtcccttc ggggtcgcga gcgactcggt gtaggccagc
      721 gagacgactg cgggcgccgc ctcggggttg aagaggacca gctcgctctc gaaggccggc
      781 gtctcgacga cgaccggcag gacgagctct cccgcgaacg cgtccgggac ggccggcacg
      841 aaggagccgt cgtgcgagag gttgtcgttg aagacggcgt aggcgccgag cggcgccgtt
      901 ccggccgcgc ggctcacgag ggcggtcgcc tcggcgaagc ccgcggcccg gaggaggtcg
      961 gcgtcgttca cttggcgcca ctcgccgggg gcgagcggcg gcagaagggc ggcggggacc
     1021 tcgtagacgg aggcgccgtc ccccgaccgg aggatgatcc tcggggtcac ggtcccttcc
     1081 gtccccatgt tcaccaccgc gagattcgac cgctccgccg ggccgctccg gaggccgaag
     1141 agccgcagcg gcgcggccgg gccctcaccc agcttccacg cggcatagga gagccccgcg
     1201 gcgccgccgc aggccgcggc tgtcgtccgc acccgggcgg aaacggtctc cgccgcaggc
     1261 gagaggccgg tgaagacgag gcggagcgag ccggcctgcc ccgggcccag gggaatcggg
     1321 agccccttcg ccgcccggag ccacgcgatt gcgtcgggaa tcacgcgctg ctcgcccggc
     1381 gcgagcgcat cggcgacggt gccgcctccc gcggctcccg ggaacatccc cgtggccggg
     1441 gtgtaggtcg cctcgaggcg cgccgcggtc gtcccgcggt tcgtgagcgt cagctccgag
     1501 cggaatcgcg cctcgccgac acccacgacg tcgaggacga cggggacgag gagctcggcc
     1561 cgcgccgccc ctgccggggc cgaccggagc gcggcgagcg cggccggcgc gtcgatccgg
     1621 ttccgggtca cgccgttgcg cgggtcgagg atcgccttcc cggtctcgcg cagcgcggcc
     1681 ttcacctcgg tcggccggcg gtccggcccg gcgccgaaga gaagcgccgc gacgccggcg
     1741 gcgtaagggg ccgcagccga ggtccctccg aaacaggagt agctcccgcc cccgtttcgc
     1801 ggcgccgtcg cgcagtgcgc gggggcgaga acgtcgaggc ggtcgccgga gtcggagtag
     1861 caggcgatcg cgtcggccgc cggcgcggag tcgctgcagc ccgatgggag gcagtgccct
     1921 cgtccgaagg cctgcgggct cacggctgcg tcgtagacgg ccccgacggc gagcgcgttg
     1981 ctcacgcacg cggggaggac gattccgccg gtgcagccgc cgttcccgct cgcggcgacg
     2041 acgaggatcc ccgcctgcgc gaggaggtcg aaggccgcct tgtagtcggg ggcgaggaga
     2101 tcgcagcggc ccggctcggg aggggccgcg tcgtcgaaat agccgccgag ggagaggttc
     2161 acgacgcgga tcgcgtgggg gttcccttcg gcgacgctcg cgaggacggc gtcgaccccc
     2221 tcgaggacct gggcgtccga cccctcgcca cgcgtgtcga ggacccgcac cgcggcaacc
     2281 ttcgcgccgc gcgcgactcc cgtcgtcagg cccgcgatga tccccgccac gggcgtgccg
     2341 tggccgtagc cgtcgcgcgc gtcgccgtcg tggtcgatcg cgtcgtagag gttgacgacc
     2401 ttcgtcccga gaggcagctc ggggtgggtg tagtcgatcc cgctgtcgag gaccgcgatc
     2461 gtgacccccg ctccgtcgac gcccgccgcc cggagctcgg gcacccgcat cagaccttct
     2521 ccctcggcgt ccagcgcccg cacgcgcccg ttcggacgga cgcgctcgat gcgggggtcc
     2581 ccggcgagcg ctgccaccgc ctttcgcggc gcgcggagga cgagcacggg gacatacgcg
     2641 aaggcctgct cgacccgcac cccgaacggc gcgtagtcgg tctcgagctc ccccgcgagc
     2701 cgggcctgga gggccgttct cgcgccggcc cggggcgagc ccgccccgag ggcgagggct
     2761 tcggccggga gccgaagagt cgcgagcacc tcgagatcct cctcgccggc cgcggcgggt
     2821 gcgagcagcc gggggtcggc cttctcgaga ggtccgcgcg cgtcggggcg cgcccggagc
     2881 cactcctcgg gaaacggcgg cgcggcggtc gccggagagg cggcggcgag cgcgaggaac
     2941 gcggcgaccg cgacggtgag gagcggcttc atggtccccg atcgtaagtg acggggacgt
     3001 agctggcctc acccgggggc gacttcgttc cgccgggtac gacgggaacg ccgggaggag
     3061 acggccgcgt gccgattgcc gtccgacagg agcgccgagc cggagcggcc cgtcggtcga
     3121 gggcgacggg caccgccacc gcggcccggc ggaacctgat gaaaacacat cagtcgagat
     3181 tccttcacag gaccattccg gtgcctttac ccgattccac ggaacgcgcg cgcgagagat
     3241 gatgcacggg ccgcggcggg cccttcgctg cccgcgcgcg atcccacagg aggatgtctt
     3301 catgacgacg agaccgatgg tcaccctgga cgggaacgag gcggtcgcct cggtcgccca
     3361 cagggtcagc gaggtgatcg cgatctatcc gatcaccccg tcatccacga tgggcgagtt
     3421 ctccgacgaa tggtccgccc agggccggaa gaacgtgtgg ggcaccgtcc cgctcgtctc
     3481 cgagatgcag tcggaggggg gcgccgccgg cgccgtccac ggggccctcc aggcgggagc
     3541 gctgacgacc accttcacgg cgtcgcaggg cctcctcctg atgatcccga acatgtacaa
     3601 gatcgcgggc gagctgacgc cgttcgtcat gcacgtctcc gcgcgctgcg tggcgacgca
     3661 cgccctgtcg atcttcggcg accattccga cgtcatggcc tgccgtcaga ccgggttcgc
     3721 gatgctggcg tccgggtcgg tccaggaggc gcacgacctc gcggcggtcg ctcaccgcgc
     3781 gacgctgatg tcgaagctcc ccttcctcca cttcttcgac ggcttccgga cgtcgagcga
     3841 ggtggcgaag atcgagcgcc tcgacgacga ggacctcctc gcgatggtga gcgaggacct
     3901 cgtctccgcg cagcgcaagc gcgcgctcac gcccgacgcg ccggtcctcc gaggctcggc
     3961 ccagaacccc gactgcttct tccaggcgcg cgaggcctgc aacggctact acgacgccgc
     4021 gcccggcctc gtccaggagg cgatggacgc gttcgcgaag ctgacgggcc gcgcctatcg
     4081 gctcttcgac tacgtcggcc acgccgaggc ggagcgggtg atcgtgatca tgggctcggg
     4141 cgccgaggtg acgcacgaga cggtcgatca cctcgtcgcc cggggcgaga aggtcggcgt
     4201 cctgaaggtc cggctctacc ggcccttcga cgcctccgcg ttcctcggcg cgctgccgaa
     4261 gagcgtgaag gcgatcgccg tcctcgaccg gacgaaggag ccgggcgccg tcggcgagcc
     4321 gctcctccag gacgtcctca cggtcctccg cgacgccgag gagacgggga cctccccctt
     4381 cgccgcctcg ccgaaggtcg tcggcggccg ctacggcctc gcctcgaagg agttcacccc
     4441 cgccatggtc aagggggtct tcgacgagct cgcgcaggcg aagccgcggc gccgcttcac
     4501 cgtcggcatc gtcgacgacg tgacgcacct ctcgctcaag tgggacgccg agttcgacac
     4561 cgagccggcc gaggtcaagc gggccgtctt cttcggcctc ggcgccgacg ggacggtcgg
     4621 cgcgaacaag aactcgatca agatcatcgg cgaggagacc gacaactacg cccagggcta
     4681 cttcgtctac gactcgaaga agtcgggcgg gatcacgatc tcccacctgc gcttcgggcc
     4741 gaagccgatc cgatcgtcgt acctcgtgtc gaccgccgga ttcgtcgcgt gccaccagtt
     4801 cggcttcctc gagaagtacg acgtcctcga gtacgcggcg cccggcgcgg tcttcctcct
     4861 gaactccccc tacggccccg atgaggtctg ggacaagctc cccgtcgagg tccaggccca
     4921 gatcctcgag aagaagctcc ggttcttcgt catcgacggc tacgaggtcg gcgcgaagac
     4981 cggcatgggc cgccggatca acacggtcat gcagacctgc ttcttcgcga tctccggcgt
     5041 cctcccccgc gaggaggcga tcgcccagat caagaagtcg atcgagaaga cgtacggcaa
     5101 gaagggcgcc gagctcgtga ggatgaactt cgaggccgtc gaccagacgc tcgagaacct
     5161 cttcgaggtg aaggtcccgt cggcggcgac gtcgaagctg aagcggcccc cgatcgtccc
     5221 gcaggaagcg ccggacttcg tccagaagat caccgcgatc atgctcgcga acaagggcga
     5281 cctcctcccc gtctcggcct tcccggtcga cgggacgttc gcgacggcga cggcccgctg
     5341 ggagaagcgg aacatcgcca tcgagatccc ggtctgggac gagaagctct gcatccagtg
     5401 caacaagtgc gcgatggtct gcccgcacgc ggcgatccgc gccaaggtct tcgacccggc
     5461 ggtcctcgag aaggcccccg ggacgtacaa gtcggtcgac taccgggctc ccgagttcaa
     5521 ggggatgaag tacacgatcc aggtcgcccc ggaggactgc acgggctgca
//
LOCUS       DAOTEZ010000287         5564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_10467_length_5564_cov_5.101289, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000287 DAOTEZ010000000
VERSION     DAOTEZ010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10467_length_5564_cov_5.101289"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2920
                     /locus_tag="P5164_14030"
     CDS             <1..2920
                     /locus_tag="P5164_14030"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRY45059.1"
                     /translation="AGSTFWVPVFGEALASFRMTARVPEGWQLVSQGDGTARGADGLA
                     RWDSKGPVDEAYLVGGPLTAYRDRAGDVETLAFLRTKDDALAAKYLAATAQYLEMYRG
                     LIGPYPYGKFALVENFWETGYGMASFTLLGPQVIRFPFILASSYPHEILHNWWGNSVF
                     VDYAKGNWCEGLTAYLADHLIQEQRGKGEEYRRGTLQKYLSYVRDGRDFPLTEFRGRE
                     SAATEAVGYGKALMGFHMVRRHLGDAAFRKTLARFYADFRGKRATFDDFRRTAETVAG
                     KPLSALFDPWVTRPGAAELALSPATVAKTDAGWTVSGTLRQAQNDEPFALEVPVAVVT
                     EKGTVRKVVPLDGREARFEVATDAEPLGLAADPQFDLFRRLDPREIPPSIGQVFGEPK
                     VLAILPAGLSAEEDAAWRALLAGWKTPTHAIEVKLETEVETLPADRAVWIVGKANRFA
                     SLFAKQEGLALGAIDLTALGETVALAGHTFAVVTRHPGDPGKAAGWVFGEPLAALPGL
                     GRKLPHYGKYSYVGFDGTEPVNVLKGQWPESDSPLRADLRSAERRGTPMPAFAPEARK
                     ALAELPPAFSQARLAAHVAALAAPALEGRGLGTPGEKSAAEYVAAEMKAAGLFPGGDD
                     GGFFQRFRVASTASGAPADVVNVVGLIPGTDPAWKDESVVVVAHYDHLGRGWPDARKE
                     FAGQAHPGADDNASGVAVLLELARAMKDEKPRRTVVFLAPTGEEAGRLGSKRWVAGPS
                     SRSAAKTLAAVNLDTVGRLGAGKLQVLGTGTAGEWIHAVRGASWVTGVETRAVPETLE
                     SSDHVSFVEAGVPAVQLFTGAHIDYHRPSDTAEKVDVPGLAKVAAVAKELASWLAGRE
                     QPLAVSLPRKDGTAAAGAAAPQGGAPGQGRRVSFGTMPDFAFEGPGVKVDAVVPGSPA
                     EKAGVRAGDVLLRIDGKEVASLRDYSERLKAARPGQTVKATLRRDGKEIEVDVTLAER
                     "
     gene            2983..4596
                     /locus_tag="P5164_14035"
     CDS             2983..4596
                     /locus_tag="P5164_14035"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRY45060.1"
                     /translation="MHAPRLLLIVSLAGFVLVAPAAAETVPGASDLIGPAAPTPTPAP
                     EAAPDSPRATVAEYLGLARSGRFDEAARFLDLPDTLARRGPELARKLKLVLDRRLWID
                     LGKLSPLPAGHREDGLSADVEQIGEIIGPTGRPEPIRLVKRERGGGPVWLFSRATSAR
                     IPDWYDRLDNRGLLDRIPAPLLRPGPKELLWWQWIVVLALLLPAWWGGKLLGWAVRKV
                     LLRFAARTATTWDEAFLVKMRGPFTLAFTLVLLRAGLPFLGLYAPAEAFALAALKAGS
                     IVAAFWAVLRAVTVSGEVLAAAPWAVASSAARSSLHFAVQVARVLVVIVGLLAGLAAL
                     GVPVNSVIAGLGIGGVAIAFAAQKTVENFFGAVSLGVDQPLRVGDFVKVGDVTGTVER
                     IGLRSTRLRTLDRTLVTIPNGKLADMQIETFAERDRIRLAAILNLEYGTTAAQMREVV
                     SGIEAMLRSHPKIWPDTVVVRFVAFSGSSLDVEVMAWFQTTSFDEFRDIRQEALLAIL
                     EIVEKAGTSFAFPTRKVHLVGPGAASFPR"
     gene            complement(4645..5172)
                     /locus_tag="P5164_14040"
     CDS             complement(4645..5172)
                     /locus_tag="P5164_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45061.1"
                     /translation="MAGAGFASILAIAVPLDGSSRVIAGGPVVPAPEDAPTPTTLPPG
                     WPGETPDLETPRQLDVWSVVHAACRRHGIEDEAVTMYHVLWEESTLRGNVSSPCGRYH
                     GIGQFTLSTFRESVGKMRRLRLIWGDEEWSPFNPVQAIEVMAFMWSRGYHDHWGPYRR
                     VVRQQARALAAANLN"
BASE COUNT          749 a         2038 c         1967 g          810 t
ORIGIN      
        1 cgccgggtcg acgttctggg tccccgtctt cggcgaggcg ctcgcgagct tccgaatgac
       61 ggcgagagtc ccggagggat ggcagctcgt ctcgcaggga gacggcaccg cccgcggggc
      121 cgacggcctc gcgcggtggg actcgaaggg ccccgtcgac gaggcctacc tcgtcggcgg
      181 cccgctcacg gcctaccggg acagggcggg cgacgtcgag acgctcgcct tcctccggac
      241 gaaggacgac gcgctcgcgg cgaagtacct cgccgcgacg gcgcagtacc tcgagatgta
      301 ccgcggcctg atcgggccgt acccgtacgg gaagttcgcg ctcgtcgaga acttctggga
      361 gacgggctac gggatggcct cgttcacgct cctcgggccg caggtgatcc gcttcccctt
      421 catcctggcg tcgtcgtacc cgcacgagat cctccacaac tggtggggga actccgtctt
      481 cgtcgactac gcgaagggga actggtgcga ggggctgacg gcctacctcg ccgaccacct
      541 gatccaggag cagcgcggca agggggagga gtaccgccgc gggacgctcc agaagtacct
      601 cagctacgtc cgcgacgggc gcgacttccc gctcaccgag ttccgcgggc gcgagagcgc
      661 ggcgacggag gccgtcggct acgggaaggc cctcatgggc ttccacatgg tccggcgcca
      721 cctcggcgac gccgctttcc ggaagacgct cgcgcggttc tacgccgact tccgcgggaa
      781 gcgcgcgacg ttcgacgact tccggaggac ggccgagacc gtcgccggca agcccctctc
      841 cgccctcttc gatccgtggg tgacgcgccc cggcgcggcc gagctcgcgc tctctccggc
      901 gacggtcgcg aagacggacg cggggtggac cgtgagcggg acgctccggc aggcgcagaa
      961 cgacgagccg ttcgccctcg aggtgcccgt cgccgtcgtc acggagaagg ggacggtccg
     1021 gaaggtcgtc ccgctcgacg ggcgcgaggc ccgcttcgag gtcgccacgg acgccgagcc
     1081 gctcggcctc gcggccgacc cgcagttcga cctcttccgc cggctcgacc cgcgcgagat
     1141 cccgccctcg atcgggcagg tcttcggcga gccgaaggtc ctcgcgatcc tccccgccgg
     1201 gctctccgcc gaggaggacg cggcgtggcg cgcgctcctc gcggggtgga agacgccaac
     1261 gcacgcgatc gaggtgaagc tcgagaccga ggtcgagacg ctgccggcag atcgcgcggt
     1321 ctggatcgtc gggaaggcga accgcttcgc ctcgctcttc gcgaagcagg agggcctcgc
     1381 cctcggcgca atcgacctga cggcgctcgg cgagacggtc gcccttgcag gacacacgtt
     1441 cgccgtcgtg acgcgccacc cgggcgatcc cgggaaggcc gccggctggg tcttcggcga
     1501 gccgctcgcc gctcttcccg gcctcggccg gaagctcccc cactacggca agtactccta
     1561 cgtcggcttc gatgggaccg aaccggtgaa cgtcctcaag ggtcagtggc ccgagagcga
     1621 ttcgccgctc cgcgccgacc tccgctccgc ggagaggcgc gggacgccga tgccggcgtt
     1681 cgcgcccgag gcgcggaagg cgctcgccga gctcccgccc gccttctcgc aggcgcggct
     1741 cgccgcgcac gtcgcggccc tcgccgcgcc cgctctcgag gggcgcggcc tcgggacgcc
     1801 cggcgagaag agcgctgcag agtacgtggc cgccgagatg aaggccgcag gcctcttccc
     1861 cggcggggac gacggcggct tcttccagcg cttccgcgtc gcctcgaccg cgagcggggc
     1921 gccggccgac gtcgtcaacg tcgtgggcct tattccaggc accgacccgg cgtggaagga
     1981 cgagtcggtc gtcgtcgtcg cgcactacga ccacctgggc cggggctggc ccgacgcgcg
     2041 gaaggagttc gcggggcagg cccatccggg cgcggacgac aacgcgagcg gcgtcgccgt
     2101 cctcctcgag ctcgcgcgcg cgatgaagga cgagaagcct cggcggaccg tcgtcttcct
     2161 cgccccgacg ggcgaggagg cggggcgcct cggctcgaag cgctgggtcg ccggaccgtc
     2221 ctcgcgctcc gcggcgaaga ccctcgccgc ggtgaacctg gacacggtcg ggcgcctcgg
     2281 cgccgggaag ctccaggtgc tcggcaccgg gaccgcgggc gagtggatcc acgccgtccg
     2341 cggcgcgagc tgggtgacgg gcgtcgagac gagggcggtc cccgagacgc tcgagtcgtc
     2401 ggaccacgtc agcttcgtcg aggcgggggt cccggcggtc cagctcttca ccggcgcgca
     2461 catcgactac caccgcccct ccgacacggc ggagaaggtc gacgtccccg gcctcgcgaa
     2521 ggtcgccgcg gtcgcgaagg agctcgcctc ctggctcgcc gggcgggagc agccgctcgc
     2581 tgtctccctc ccgaggaagg acgggactgc cgccgcgggc gccgccgcgc cgcagggcgg
     2641 cgccccaggg caggggcgtc gcgtcagctt cgggacgatg cccgacttcg ccttcgaggg
     2701 acctggcgtg aaggtggatg cggtcgtccc cggatccccc gccgagaagg ccggcgtcag
     2761 ggccggcgac gtcctcctgc ggatcgacgg gaaggaggtc gcgagcctcc gcgactactc
     2821 cgagcggctg aaggccgcgc ggcccgggca gaccgtgaag gcgacgctcc ggcgtgacgg
     2881 gaaggagatc gaggtcgacg tgacgctcgc cgagcgatga cggctcaccg cgcggggcgg
     2941 ccgtggcttg acccggccgg acccgcgtgc tgtattgggc gcgtgcacgc accgcgtctc
     3001 cttctgatcg tctctctggc gggattcgtc ctcgtggccc cggcggcggc cgagaccgtt
     3061 cccggcgcct ccgacctcat cggtcccgcc gctccgacgc ccacgcccgc accggaggcc
     3121 gcccccgact cgccgcgcgc gacggtcgcc gagtacctcg gcctcgcgcg ctccggccgg
     3181 ttcgacgagg cggcgcgttt cctggacctt cccgacacgc tggcccggcg cggccccgag
     3241 ctcgcccgca agctgaagct cgtcctcgac cggcggctct ggatcgacct cgggaagctc
     3301 tccccgctcc cggcaggcca ccgggaggac ggcctctccg ccgacgtcga gcagatcggc
     3361 gagatcatcg gaccgaccgg ccgccccgag ccgattcggc tcgtgaagcg ggagcgcggc
     3421 ggcggcccgg tctggctctt ctcccgtgcg acctccgccc gaatcccgga ctggtacgac
     3481 cggctcgaca accgcggcct gctcgaccgc atccccgcac ccctccttcg cccgggcccg
     3541 aaggagctgc tctggtggca gtggatcgtg gtcctcgccc tcctcctgcc cgcctggtgg
     3601 ggcgggaagc tcctcggctg ggcggtccgc aaggtgctcc ttcgtttcgc ggcgcggacc
     3661 gccacgacct gggacgaggc cttcctcgtc aagatgcgcg ggccgttcac gctcgccttc
     3721 acgctcgtcc tgctccgggc cggtcttccg ttcctcggcc tctacgcacc cgccgaggcg
     3781 ttcgccctcg ccgcgctgaa ggcaggcagc atcgtcgccg cgttctgggc cgtcctccgc
     3841 gcggtgaccg tctccggaga ggtcctggcc gccgccccct gggccgtcgc gagctcggcc
     3901 gcgcgctcct cgctccattt cgccgtccag gtcgcgcggg tgctcgtcgt catcgtcggg
     3961 ctcctggccg gcctcgcggc gctcggcgtc ccggtgaaca gcgtcatcgc cgggctcggc
     4021 atcggcggcg tggcgatcgc gttcgccgct cagaagaccg tcgagaactt cttcggcgcg
     4081 gtctcactcg gcgtggacca gccgctgcgc gtcggggact tcgtcaaggt gggggacgtc
     4141 accgggaccg tcgagcggat cggcctccgc tcgacacggc tgcggacgct cgaccggacg
     4201 ctcgtgacga tcccgaacgg gaagctcgcc gacatgcaga tcgagacgtt cgcggagagg
     4261 gaccggatcc gcctcgccgc gatcctgaac ctcgagtacg ggacgacggc cgcgcagatg
     4321 cgcgaggtcg tctcgggaat cgaggcgatg cttcggtccc atccgaagat ctggcccgac
     4381 accgtcgtcg tccgcttcgt cgccttctcc ggctcctccc tcgacgtcga ggtgatggcc
     4441 tggttccaga cgacgagctt cgacgagttc cgcgacatcc ggcaggaagc cctgctcgcg
     4501 atcctggaga tcgtcgagaa ggccggaacg agcttcgcgt tcccgactcg gaaggtccat
     4561 ctcgtcgggc cgggagccgc ctcgttcccg aggtgagacg aggggccgcg cgaccggctc
     4621 cgagatcgga tcgggagcgg ccggtcagtt caggttcgcg gccgccaggg cccgggcctg
     4681 ctgtcggacg acccgacggt acggccccca gtggtcgtga tacccccggc tccacatgaa
     4741 ggccatgacc tcgatggcct ggaccgggtt gaacggggac cactcctcgt ctccccagat
     4801 cagccggagg cgtcgcatct ttccgacgct ctctcggaac gtcgagaggg tgaactgacc
     4861 gatgccgtgg tagcgcccgc acggggagct gacgttcccc cggagcgtgc tctcctccca
     4921 gaggacgtgg tacatcgtca cggcctcgtc ctcgatcccg tgcctccggc acgccgcgtg
     4981 gacgaccgac cagacgtcga gctggcgagg ggtctcgaga tccggcgttt cgccgggcca
     5041 tccgggcgga agcgtcgtag gggtcggggc gtcctcgggg gccgggacga ccggtccccc
     5101 ggcgatgact ctcgagcttc cgtccagcgg aacggcgatt gcgagaatcg acgcgaagcc
     5161 tgctcccgcc atccggagaa tcgttccccg gctgcgccgt tcgcccgctt cgctcggttc
     5221 ctcatctcgt cggtccataa atctccatct atcagtgatt tgggtgtctc atcggggaaa
     5281 atgccgaacg cacgccagga actgacgact gcgagggatc ccctgaggat gaccggaata
     5341 tggggcatgc cgccggacac gtcaagtgag cgccggcaca gccatggagg ctcggttcaa
     5401 cctcggagcg cgcccgtttc cgaggttccg cccgagcgat tcagtaccgg cgcatgaggc
     5461 cgacgacgat tccctggacc tggacgtcct cttcgcgggc gcggatcgga gccagcctct
     5521 cgttcgaagg ctgcaggcgc acgactccct cgggctcgcg gtag
//
LOCUS       DAOTEZ010000288         5543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_10521_length_5543_cov_7.054118, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000288 DAOTEZ010000000
VERSION     DAOTEZ010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10521_length_5543_cov_7.054118"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1864
                     /locus_tag="P5164_14045"
     CDS             <1..1864
                     /locus_tag="P5164_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45062.1"
                     /translation="LVARFAAEKERMRALRAESPYFPRLLQPDGGHEGTLPALVYDKP
                     SRSFFVPPCAACGRPLELLTDEQFLRERRLPSYLESLHRLVGCASCATTDRVIAVFSY
                     APLPGEADVPVGGPDELLKGLSRALLRDWDESQVKAFASRESAEEALRLRASGGGSLG
                     DFAARWSPFSFCPSPFLVSGLSPVRWDEWADFVGGRTEEDLVSAAAPESLETQSARHR
                     LEWLRTSLPPTGRFLYGHDGSGVDAIEVLYLKMTAFRQLAAGLLSFYRATGQPHLDLH
                     PGHVLLDSYGTGASLPALWTFQARLHGLAAASRTSSFGVEVVVPPPEPMFPYAAPEIL
                     EFRLASRRPGDVYLSEVEPADAGLGVKVEGRLSDPNGLFPRPEEPDWIHVTFPDEALG
                     LGIDALVVRRKPDVPASYEELTFVSEPIELDDAAVKRLRRSFGVRLPGARYKVYPRFG
                     APSDLYSLGVLLLRSLAGGERTELPELLQGVQRAAAAAVRSKDGDPLARVRAALAAEA
                     STATLLDRSRVFWKEEDRVPGRPNAIPPPLFERALLLALRLLTRIPGFSIAIGPGDFV
                     AEDPTGRVEQALRETDEVVAELRALLVHRQPLHFEIQDVLAELLEAESETAPRT"
     gene            1983..4088
                     /locus_tag="P5164_14050"
     CDS             1983..4088
                     /locus_tag="P5164_14050"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRY45063.1"
                     /translation="MSVPPRSLTLEVWLGPMEGLRYAAEARQAMQLVRIGRLPFDRQS
                     KERNHLVLSVSAGVSGSHAEVRFAQGRIFLRDLGSSNGTFAAQQAVTTETEVKSGEIF
                     LVSLTPVQVYLTSPLAAPPPFEPLPVTHWEGAAVEPLLSAAVACAAGRREGYVDTRHL
                     LDAVLALGDPEIEELLSSGGARPAEVRETLFRKPFFRDAIGWIAEILVKPVNLTGSFE
                     TAVVSPRVARLLDGTRTAVPDGVAGDEAVRWAARSVLAGLVADRFGPVGDWLTSCGLT
                     AVPEGFVGEAGARAPAARSAEPELTGRASVKGGVAKGRATTLLTEDVGEATARGGPSP
                     LSRTLPPPPPPPPATSPETSAVAPASVRHREPEPEQRTEAAPAARHEPPREPEPLPAP
                     PPATVAAPFSWGAARTPAEILLDGRARELADELFALAGELRFGTSDDRRRQLKRRVEK
                     ELAPLPKESRARFLELLRRHFPVLPAGAGADPEVARLRRRLEESERKRVEESPSKSVE
                     RRKPPASAAPSGPLPLKQLVHGEDLAQADRNVVVLRRVIEYALAMEGMTLGLIQSLTM
                     PGNETMAFKLPGFKETLRHFLHAIEQGKPVSVQRVEEYLEELKKWQVAILAAFHQAPG
                     EWFDRLWKRINPVTIESAPRSAGWKLRGEAAEWWDLYKIAVRDLSPDLVNDQILQGVA
                     RLSKEELEKLRKTQRGEHS"
     gene            4085..4567
                     /locus_tag="P5164_14055"
     CDS             4085..4567
                     /locus_tag="P5164_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45064.1"
                     /translation="MTKTPSRFLLRIAACGLLLGALPALTGCASKVGTISFRCGAQIN
                     GGLALAVDVIRATEEESRKIQEMGEKWFYDQTRFNIQGQNRIQTVTFTTGEGAGACDK
                     TVSVPFSKGERFLVLIADYKFQNPDPTKHVVVLSREKWVGQKILVSVLERELAVERRW
                     "
     gene            4577..>5543
                     /gene="tssK"
                     /locus_tag="P5164_14060"
     CDS             4577..>5543
                     /gene="tssK"
                     /locus_tag="P5164_14060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HRY45065.1"
                     /translation="MKKTPQVLWAEGMFLRPHHLQTAERYREEALQREIQRIQPFYFG
                     LQSLDVAPDQLENFVFEIRDVSLKLKDGTALSSDSTLRLSPRSFKNELDKAGGRMRVF
                     VGVPVMRENAPNTFAPGEGPAGVDRRYVAENAEALDENTGSNPQPVGVRRCNGKLFFG
                     AESQEGYECLEVAVVERSGSGRNFPVLSREFIPSLTELGASRTIQALCDGVTNRIEAK
                     HRLLLTDVAQGRLAITSDGTSGWQAIIKLQILGSFLFLFQQLTKIPRIHPFHVYTEFA
                     RLAGELSIFDDGKVPVRIPVYDHEKLGPCFHETCRVIEALLEKIVA"
BASE COUNT          741 a         2075 c         1909 g          818 t
ORIGIN      
        1 cctcgtcgcg cgcttcgccg cggagaagga gcggatgcgc gccctgcgcg ccgagagccc
       61 gtacttcccg aggctcctgc agccggacgg cggccacgag gggacgctcc cggccctcgt
      121 ctacgacaag ccctcgcgct cgttcttcgt cccgccgtgc gccgcgtgcg gccggccgct
      181 ggagctcctc accgacgagc agttcctgcg cgagcggcgg ctgccgtcgt acctcgagtc
      241 gctccaccgc ctcgtcggct gcgcgagctg cgccacgacg gaccgcgtca tcgcggtctt
      301 ctcctacgcg cccctcccgg gcgaggccga cgtcccggtg ggcgggcccg acgagcttct
      361 gaaggggctc tcccgggcgc tcctccggga ctgggacgag agccaggtga aggcgttcgc
      421 ctcccgcgaa tcggccgagg aggccctccg tctccgcgcc tcgggcggcg gctccctcgg
      481 agacttcgcc gcgcggtgga gcccgttctc gttctgtccc tccccgttcc tcgtgagcgg
      541 cctctccccg gtgcgctggg acgagtgggc cgacttcgtc ggcggtcgca cggaggagga
      601 cctcgtctcc gccgccgcac cagagagcct cgagacgcag tcggcgcgcc accggctcga
      661 gtggttgcgc acgtcgcttc cgccgaccgg gcgcttcctc tacggacacg acggatcggg
      721 cgtcgacgcg atcgaggtcc tctacctgaa gatgacggcc ttccggcagc tcgccgcggg
      781 cctcctttcg ttctatcgcg ccaccgggca gccgcacctc gacctgcacc cggggcacgt
      841 cctcctcgac agctacggca ccggcgcctc gctcccggcc ctctggacat tccaggctcg
      901 gctgcacggt ctggccgcgg cctcgaggac gtcctcgttc ggcgtcgagg tcgtcgtccc
      961 gccgccggag ccgatgttcc cgtacgcggc tcccgagatc ctcgagttcc gcctcgcctc
     1021 gcgccgtccc ggcgacgtct acctctccga ggtcgagccg gcggacgcgg ggctcggcgt
     1081 gaaggtcgag gggcggctca gcgacccgaa cggcctcttc ccgaggccgg aagagcccga
     1141 ctggatccac gtcacgttcc ccgacgaggc gctcggcctc gggatcgacg ccctcgtcgt
     1201 gaggcggaag cccgacgtcc cggcctccta cgaggagctg acgttcgtct ccgagccgat
     1261 cgagctcgat gacgccgcgg tgaagcggct ccgccgctct ttcggagtgc gcctccccgg
     1321 cgcgcgctac aaggtctacc ctcgcttcgg cgcgccgtcc gacctctact ccctcggcgt
     1381 cctcctcctg cggtccctcg ccggcggcga gcggaccgag ctgccggagc tcctgcaggg
     1441 ggtccagcgg gccgccgcgg cggccgtccg ttcgaaggac ggggaccccc tcgcgcgcgt
     1501 gagggcggcc ctggccgcgg aggcgtcgac cgcgacgctc ctcgatcgct cccgcgtctt
     1561 ctggaaggag gaggaccgcg ttccgggccg gccgaacgcg atcccgccgc ccctgttcga
     1621 gagagcgctc cttctcgcgc tccgtctcct gacgcggatt ccgggtttct cgatcgcgat
     1681 cggtccggga gacttcgtgg cggaggaccc gacgggccgc gtcgagcagg cgcttcggga
     1741 gacggacgag gtcgtcgccg agctccgggc gctcctcgtc caccgccagc cgctgcattt
     1801 cgagatccag gacgtcctcg cggagctgct cgaggcggag tcggagacgg cgccgcgcac
     1861 ctgacgccgg gccgggtcgc ggggtttccg gaccgagggt aatatccggc cactgtgtcc
     1921 gacaggtcgt ccgtctcctc gttcgaggtg ctggcgtgtc gccccttcgc ggaggggcgc
     1981 ccgtgagcgt cccgccgcgc tccctcacgc tcgaggtctg gctcggcccg atggaggggc
     2041 tccgctacgc cgccgaagcg cggcaggcga tgcagctcgt ccgcatcggc cgccttccgt
     2101 tcgaccggca gtcgaaggag cggaaccacc tcgtcctctc cgtctccgcc ggcgtctccg
     2161 gctcgcacgc ggaggttcgc ttcgcgcagg gccggatctt cctcagggac ctcggcagct
     2221 cgaacgggac gttcgcggcc cagcaggcgg tgacgaccga gaccgaggtg aagtcgggcg
     2281 agatcttcct cgtctcgctg acgccggtgc aggtctacct gacgagtccg ctcgcggcgc
     2341 ccccgccctt cgagccgctt cccgtgacgc actgggaagg cgccgccgtc gagccgctcc
     2401 tttccgcggc ggtcgcctgc gcggccggga ggcgggaggg atacgtcgac acgcgccacc
     2461 tgctcgacgc ggtcctcgcg ctcggcgacc cggagatcga ggagctcctc tcgagcggcg
     2521 gcgcgcggcc ggcggaggtc cgcgagactc tcttccggaa gcccttcttc cgcgatgcga
     2581 tcggctggat cgccgagatc ctcgtcaagc cggtcaacct gacggggagc ttcgagaccg
     2641 ccgtcgtctc tccccgcgtc gcgcgcctcc tcgacggcac ccgcaccgcc gtccccgacg
     2701 gggtcgcggg cgacgaggcg gtccgctggg ccgcccgaag cgtcctcgcc gggctcgtcg
     2761 ccgaccggtt cgggccggtc ggcgactggc tgacttcgtg cggcctcacc gccgtcccgg
     2821 aagggttcgt cggggaggcg ggagcgcggg ctccggccgc tcgctccgcg gagccggagc
     2881 tcaccggtcg ggcttctgtg aagggcggcg ttgcgaaggg gcgggccacg acgctcctga
     2941 cggaagacgt cggcgaggcg acggcaaggg gcggaccgtc cccgctctcg cggacgcttc
     3001 cgcctccgcc gccccctccc cccgcgacgt caccggagac ctccgcggtc gctccggctt
     3061 ccgttcggca ccgtgagccg gagccggagc agcggacaga ggccgccccg gccgcgcggc
     3121 acgagccgcc gcgcgagccg gagcccctcc ccgcacctcc gcccgcgacc gttgcggctc
     3181 cgttctcgtg gggcgcggcg cggaccccgg ccgagatcct cctcgacggc cgggcgcgcg
     3241 agctcgccga cgagctcttc gcgctggcgg gggagctgcg gttcggaacc tccgacgacc
     3301 ggcgccgcca gctgaagcgg cgcgtcgaga aggagctcgc cccgctcccg aaggaatccc
     3361 gtgcgcggtt cctggagctc ctccggcgcc atttcccggt gctgccggcg ggcgccgggg
     3421 ccgaccccga ggtggcccgg ctccgtcgca ggctcgagga gtcggagcgc aagcgcgtcg
     3481 aggagagccc gtcgaagagc gtcgagcgcc ggaagccccc agcgtcggcg gcgccctccg
     3541 ggccgcttcc cctcaagcag ctcgtccatg gcgaggacct cgcgcaggcg gaccggaacg
     3601 tcgtcgtcct ccgtcgcgtg atcgagtacg cgctcgcgat ggaggggatg acgctcggcc
     3661 tcatccagtc gctcacgatg ccggggaacg agacgatggc cttcaagctc cccggcttca
     3721 aggagacgct ccgccacttc ctccacgcga tcgagcaggg caagccggtc agcgtccagc
     3781 gggtggagga gtacctcgag gagctgaaga agtggcaggt cgcgatcctc gcggcgttcc
     3841 accaggcccc gggggagtgg ttcgaccgcc tctggaagcg gatcaacccg gtcacgatcg
     3901 agtccgcgcc ccggagcgcc ggctggaagc tccggggcga ggcggcggag tggtgggacc
     3961 tctacaagat cgcggtccgg gacctctctc ccgacctggt caacgaccag atcctccagg
     4021 gcgtggcccg actttcgaag gaagagctcg agaagctcag gaagacacag cgaggagaac
     4081 actcatgacg aagacgccgt cccgcttcct gctccggatc gccgcctgcg gcctgctcct
     4141 cggggcactc cccgcgctca ccggctgcgc gtcgaaggtc gggacgatct cgttccgatg
     4201 cggcgcccag atcaacggcg ggctcgccct cgccgtcgac gtcatccgcg ccacggagga
     4261 ggagtcccgg aagatccagg agatggggga gaagtggttc tacgaccaga cccgcttcaa
     4321 catccagggc cagaaccgga tccagaccgt gacgttcacg accggcgagg gcgccggcgc
     4381 ctgcgacaag accgtctccg tgccgttctc gaagggcgag cggttcctcg tcctgatcgc
     4441 cgactacaag ttccagaacc ccgacccgac gaagcacgtc gtcgtcctct cccgcgagaa
     4501 gtgggtgggg cagaagatcc tcgtctcggt cctcgagcgg gagctcgccg tcgagcgccg
     4561 ctggtaacgg ggccccatga agaagacgcc gcaggtcctc tgggccgagg ggatgttcct
     4621 tcggccccac cacctccaga cggccgagcg ctaccgcgag gaagccctcc agcgcgagat
     4681 ccagaggatc cagccgttct acttcgggct ccagtccctg gacgtggcgc cggaccagct
     4741 cgagaacttc gtcttcgaga tccgcgacgt ctccctgaag ctgaaggacg gcaccgcgct
     4801 gtcgtccgac tccacgctcc gcctctcccc ccggagcttc aagaacgagc tcgacaaggc
     4861 gggcgggcgg atgcgcgtct tcgtcggcgt gccggtgatg cgggagaacg cgccgaacac
     4921 gttcgccccg ggcgaggggc cggcgggcgt cgaccggcgc tacgtcgccg agaacgccga
     4981 ggccctcgac gagaacaccg ggtcgaaccc gcagccggtc ggcgtgcggc gctgcaacgg
     5041 caagctcttc ttcggcgccg agagccagga gggctacgag tgcctcgagg tcgccgtcgt
     5101 ggagcgctcc ggctcggggc ggaactttcc ggtcctctcg cgcgagttca tcccgtcgct
     5161 gaccgagctc ggcgcctccc ggaccatcca ggcgctctgc gacggcgtga cgaaccggat
     5221 cgaggcgaag catcgcctgc tcctgaccga cgtcgcgcag gggcgcctgg cgatcacctc
     5281 cgacgggacc tcgggctggc aggcgatcat caagctccag atcctcggga gcttcctctt
     5341 cctcttccag cagctcacga agatcccgcg catccacccg ttccacgtct acaccgagtt
     5401 cgcgcgcctc gcgggcgagc tctcgatctt cgacgacggc aaggtcccgg tccggatccc
     5461 cgtctacgac cacgagaagc tcggcccgtg cttccacgag acgtgccgcg tcatcgaggc
     5521 cctcctcgag aagatcgtcg cct
//
LOCUS       DAOTEZ010000289         5535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_10536_length_5535_cov_7.701825, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000289 DAOTEZ010000000
VERSION     DAOTEZ010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10536_length_5535_cov_7.701825"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(347..3199)
                     /locus_tag="P5164_14065"
     CDS             complement(347..3199)
                     /locus_tag="P5164_14065"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRY45066.1"
                     /translation="MRHRLLSAASFALGLALGAPPAEAVRSALRVYTSRDGLPQLQVT
                     ALCQDRAGFLWVGTLAGGVGRYDGRRFEVTDAATGLPGSSIESITCGERGEVFVSTTN
                     GGAVFAGGAWSSLPAPPGPAPAVNELLALEDGRLFAAASGGLFVARSPGAPWEKVPAA
                     GGLEGAEAVSLHRSDDGTLWVGTGRGLGRVLPGGALTGFTSNDLPRGSATVVVPGRKD
                     GLLVGLAEVGLFSLDPETGAAARVGDDAVPGRNVSQVVHEPDGEGTWIATSDRGAFRW
                     DGRSGFDRLGAAEGLPDARVWSLLVDREGIVWLGTDSGLAKRGPTAFHTFGPEDGLPE
                     ASPLYGMAETPGGVLWFGAHDRGLLRRSPDGAYRLFTSRDGLPHTEVRSFCVTPDGDL
                     VATTFRGAVRIEGDRVVPFRLPEGAPRAIDKIAYEPDGTLLLGSARQGLFLVRDGALS
                     RIGPPVGDSVSVIHVGRRGTVWVGGPGWGAVALRDDAPPQALGIEEGLPSNVVTSILE
                     DRRGGLWIATDRGIFHRTREGVPRVIDARTGLPDSYVYWVGEEADGHVWAGTNRGAAR
                     LAPSGEVRVFTTNDGLGADECNEDGFFADSKGRVWITTDGLSLFRGLPAPRQPVPPLV
                     AVSEVRVGATRVVPSRLVELPARHEPLTFRFAALSFLDEVATRFRYRLVGLSDSWTTA
                     EPGQSETTYGGLGPGSYLFEVTATTVDGREPDAPASARVSVARPWWGRLPVVLGALAG
                     VLAILAAVLRVRERRLLAARTRLEETVAERTEELRSLNAQLTELAITDPLTGLPNRRS
                     ILDSASDAFSLARRRRIPLALAMIDFDHFKAINDTLGHAEGDRLLVEGTRRMLEGLRS
                     EDDLGRYGGEEFLAVFPMTDAEGGASAGERLRRAVFAVHLSAPVPGATTRDRASVSVG
                     IATIREEDTSLDMLLRRADAALYEAKQAGRNRVVVR"
     gene            complement(3196..4320)
                     /locus_tag="P5164_14070"
     CDS             complement(3196..4320)
                     /locus_tag="P5164_14070"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRY45067.1"
                     /translation="MGSAGRMALFLGIVLSVWSAFHVYALQRAFSIPALSRAVPFAGR
                     VALFALLWLSYPAGRVLAHRAPGAVARVVEAAGATWMGVLFLAVTALAAADLLTGFGK
                     LWPGGAVPARASALGVAVLLSVVALVQTARGPRVVRHEVKVSALPDGRAELRLVQLSD
                     LHLGTLLGERWLARRIAEVEALAPDLVVVTGDLVDGEAQAVAPLVPVLRTLTAPLGVW
                     GVTGNHEYYAGLEKCLSVLAAGGIRVLRDEWAEAAPGLVLSGVDDLTARRQLGLDGRT
                     LDAAFAGIPDPSVVVHLSHSPLLVREAAARGARLMLSGHTHGGQLWPFVFLSAIPYPF
                     QAGRYEVDGMTLVVSRGTGTWGPPMRLFRRSEIVEVVLRR"
     gene            4376..>5535
                     /locus_tag="P5164_14075"
     CDS             4376..>5535
                     /locus_tag="P5164_14075"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY45068.1"
                     /translation="MTDEKQNRFPAVFWTANVTELFERAAYYSMASFVVIYLGQLGLG
                     EYWPSNLNSVLWTLVYFLPILSGTIADQVGFRKSLLVAFVLLAAGYTLMGSPVWFGGA
                     KLSATVGKEFTASSGLVGVLVAGILLIGIGGSVIKPCISGTVQKTSGALATLGFAIFY
                     MIINIGSLFGRGTAYVVRTRPDVVLILAVVAVCGVAAALLTLLVRWNVTHREKPGTIA
                     KTTLGAFAIITGAGLGISQALKLRPSPELAEGSGALSYIFAVATAASIVAFFVVLVFY
                     KEPAVAADAPVKPKRTPGRILLDMVLVLKNPRFALFLLVISGFFFLYNQVYNVMPLYV
                     KRVVETSPAMDLYTAANPFVIVVFQLLVTSLFGKMKPVKSMIVGCVIIGVSMG"
BASE COUNT          823 a         2059 c         1935 g          718 t
ORIGIN      
        1 ccaccgcgag cgccttctcg gtgatcgagc ccgactcggc gacgacgatc ttgacgccgc
       61 cgtcgtcctt ctttcccgag ctcgcgaggg cgtagatccc ggtgacgacg ccgcccagaa
      121 cgacgagccc gagaaggatc ttgaagagct tgcccatgcc cgacctccac gaccccggag
      181 tggggcgatc cgtcccatcg gagacgttcg attcaggggt catgccgcag ttacgatgga
      241 gggccgggaa ggttccgcga cggccggacc tgtgacacaa agtcacggat cgaccgggag
      301 gaatgggccc gcctcccgat cgagggcgga cgggggcgat cgcccttcag cggacgacga
      361 cccgattccg gccggcctgc ttcgcttcgt agagcgccgc gtcggctcgg cgaaggagca
      421 tgtcgaggct cgtgtcctcc tcgcggatcg tcgcgatgcc gacgctgacg gaggcccggt
      481 cgcgcgtcgt cgcgccggga accggcgccg agaggtgcac ggcgaagacc gccctgcgga
      541 gtctctcccc tgccgacgcc ccgccctccg cgtccgtcat cggaaagacg gcgaggaact
      601 cctccccgcc gtacctgccg aggtcgtcct cgctccggag cccttcgagc atgcggcggg
      661 tcccctcgac gagcagccgg tctccttcgg cgtggcccag ggtgtcgttg atcgccttga
      721 agtggtcgaa gtcgatcatc gcgagcgcca gcgggatccg gcggcggcgc gccagcgaga
      781 acgcgtcgga cgccgagtcg aggatcgagc gccggttcgg aaggccggtg agggggtcgg
      841 tgatggcgag ctccgtgagc tgggcgttga ggctgcgcag ctcctccgtc cgctcggcga
      901 cggtctcctc gagtcgcgtg cgggcggcga ggagccgcct ctcgcggacc cggagcacgg
      961 ctgcgaggat cgcgaggacg ccggcgaggg cgccgaggac gaccggcagc ctcccccacc
     1021 acggccgggc cacgctcacg cgggcggaag cgggcgcgtc gggctcgcgc ccgtccaccg
     1081 tcgtcgccgt cacctcgaag aggtacgagc cgggccccag ccctccgtac gtcgtctccg
     1141 actggcccgg ctccgcggtc gtccaggaat ccgagaggcc gacgagccga tagcggaacc
     1201 gcgtcgccac ctcgtccagg aacgagagcg ccgcgaaacg gaacgtgagc ggctcgtggc
     1261 gggccgggag ctccacgagg cgggagggga ccacgcgggt cgccccgacc cggacctccg
     1321 acacggcgac gagcgggggc acgggctgcc gaggggcggg gagcccccgg aagagcgaga
     1381 ggccgtcggt cgtgatccag acccgtccct tcgagtcggc gaagaagccg tcctcgttgc
     1441 actcgtccgc gccgaggccg tcgttcgtcg tgaagaccct cacctcaccc gacggggcga
     1501 ggcgcgcggc gccccggttc gtgccggccc agacgtggcc gtccgcctct tcgccgaccc
     1561 agtagacgta cgagtcggga aggccggtcc gtgcgtcgat cacgcggggg acgccttccc
     1621 gcgtccggtg gaagatcccg cggtccgtgg cgatccagag ccctccccgc cggtcctcca
     1681 ggatcgacgt gacgacgttc gaggggagcc cttcctcgat cccgagcgcc tgcggcggcg
     1741 cgtcgtcccg caaggcgacg gctccccagc ccggccctcc gacccagacg gtcccacggc
     1801 gcccgacgtg gatcacggag accgagtcgc ccaccggagg accgatccgc gagagcgcgc
     1861 cgtcgcgcac caggaagagg ccctgccgcg ccgagccgag caggagggtc ccgtccggct
     1921 cataggcgat cttgtcgatc gccctcggcg ccccttcggg gaggcggaac gggacgaccc
     1981 tgtccccttc gatccgcacc gctccgcgga acgtcgtcgc cacgaggtcg ccatccggcg
     2041 tcacgcagaa cgagcgcacc tccgtgtgcg gcaggccgtc ccgggacgtg aagaggcggt
     2101 atgcgccgtc ggggctccgg cggagcagcc cccggtcgtg cgccccgaac cagaggacgc
     2161 cgcccggggt ctcggccatt ccgtagagcg gcgacgcctc cgggagcccg tcctctggtc
     2221 cgaacgtgtg gaacgcggtc gggcctctct tggcgagacc cgaatcggtc ccgagccaga
     2281 cgattccctc ccggtccacg aggagcgacc agacccgggc gtcggggagc ccctccgccg
     2341 cgccgaggcg gtcgaagccg gagcgcccgt cccagcggaa ggcccctcga tcgctcgtcg
     2401 cgatccaggt cccttcccca tccggctcgt ggacgacctg cgagacgttc cggccgggca
     2461 cggcgtcgtc gccgacccgc gcggcggccc ccgtctccgg atcgagcgag aagaggccga
     2521 cctcggccag cccgacgagg agcccgtcct tccgacccgg gacgacgacg gtcgcggagc
     2581 cccgagggag gtcgttcgac gtgaagcccg tcagcgcgcc gccggggagc actcggccga
     2641 gcccgcgccc cgttccgacc cagagcgtcc cgtcgtccga ccggtgcaac gacaccgcct
     2701 ccgctccctc gagcccgccc gcggcgggga ccttctccca gggagcgccc ggcgaccgcg
     2761 ccacgaagag ccctccggac gcggcggcga agagcctgcc gtcttccaga gcgaggagct
     2821 cgttcaccgc aggcgccggc ccgggcggcg ccgggagcga ggaccaggct ccccctgcga
     2881 agaccgcgcc tccgttcgtg gtgctgacga agacctcgcc cctctcgccg cacgtgatcg
     2941 actcgatcga ggagccaggc aggccggtcg ccgcgtccgt cacctcgaac cgcctcccgt
     3001 cgtatcggcc gaccccgccg gcgagcgtcc cgacccagag gaagccggcg cggtcctggc
     3061 agagcgcggt cacctggagc tggggaaggc cgtcccgcga cgtgtagacg cgcagcgccg
     3121 agcggaccgc ctcggccggc ggggcgccga gcgccaggcc gagcgcgaac gaggcggccg
     3181 agagaagccg gtgcctcacc tccggaggac gacctccacg atctcggatc gacggaagag
     3241 cctcatgggc gggccccacg tcccggttcc gcgcgagacg acgagcgtca tcccgtccac
     3301 ctcgtagcgc cccgcctgga acgggtacgg gatcgccgag aggaagacga acggccagag
     3361 ctgcccgccg tgcgtgtggc cggagagcat gagccgcgcg ccgcgcgccg ccgcctcgcg
     3421 gacgaggagc ggagagtggg agaggtgaac gacgacggag gggtccggaa tgccggcgaa
     3481 cgccgcgtcg agggtccggc cgtcgagccc gagctgccgc cgcgccgtca ggtcgtcgac
     3541 gcccgacagg acgaggcccg gcgcggcctc ggcccactcg tcccggagga cccggatccc
     3601 tccggcggcg aggaccgaga ggcacttctc gagaccggcg tagtactcgt ggttccccgt
     3661 cacgccccag acgccgagcg gcgcggtcag cgtccgcagg acggggacga gcggcgcgac
     3721 cgcctgcgcc tcgccgtcga cgaggtcccc cgtgacgacg acgaggtcgg gtgcgagcgc
     3781 ctccacctcc gcgatccgcc gcgcgagcca gcgctcgccg aggagggtcc cgaggtggag
     3841 gtccgagagc tggacgaggc gcagctcggc tcgaccgtcc gggagcgcgg agaccttcac
     3901 ctcgtgccgg acgacgcgcg ggccgcgcgc cgtctggacg agcgcgacga ccgagaggag
     3961 cacggcgacg ccgagcgccg aggcgcgcgc cgggaccgct ccacccggcc agagcttccc
     4021 gaaccccgtg aggaggtcgg ccgcggcgag agccgtcacc gcgaggaaca ggacgcccat
     4081 ccacgtcgct ccggccgcct cgacgacccg ggcgacagcc ccgggcgccc tgtgcgcgag
     4141 gacgcgaccc gccggatagg agagccagag cagcgcgaag agcgccacgc gcccggcgaa
     4201 cgggacggcc cgcgaaagcg cgggaatcga gaaggcccgc tggagcgcgt agacgtggaa
     4261 cgcgctccag acggagagaa cgattcccag gaagagggcc atccgccccg ccgagcccat
     4321 ccggagaagt ctacggcaga cgccgcgggc cgcgcccctc tagaatcccg aggtcatgac
     4381 cgacgagaag cagaaccgct ttcccgccgt cttctggacc gcaaacgtca ccgagctctt
     4441 cgaacgggcc gcctactact cgatggcgag cttcgtcgtc atctacctcg ggcagctcgg
     4501 gctcggcgag tactggccct cgaacctgaa cagcgtcctc tggacgctcg tctacttcct
     4561 cccgatcctc tccggcacga tcgccgacca ggtcgggttc cggaagtcgc tcctcgtcgc
     4621 gttcgtcctg ctcgcggcag gctacacgct gatgggctcg cccgtctggt tcgggggggc
     4681 gaagctctcg gcgaccgtcg ggaaggagtt cacggcctcg tccggcctcg tcggcgtcct
     4741 ggtcgcgggc atcctcctga tcggcatcgg cggctcggtc atcaagccct gcatctcggg
     4801 gaccgtgcag aagacctcgg gcgcgctcgc gacgctcggc ttcgcgatct tctacatgat
     4861 catcaacatc gggtcgctct tcggccgcgg gacggcgtac gtcgtgcgga cccgccccga
     4921 cgtcgtcctg atcctcgcgg tcgtcgccgt ctgcggcgtc gccgcggcgc tcctgacgct
     4981 cctcgtccgc tggaatgtga cgcaccgcga gaagcccggg acgatcgcga agacgacgct
     5041 cggagcgttc gcgatcatca cgggcgccgg gctcggcatc tcgcaggccc tgaagctccg
     5101 cccctcgccc gagctcgcgg aagggagcgg cgccctctcg tacatcttcg ccgtggcgac
     5161 cgcggcctcg atcgtcgcct tcttcgtcgt cctcgtcttc tacaaggagc ccgccgtcgc
     5221 cgcggacgct cccgtcaagc cgaagcgcac cccgggccgg atcctcctcg acatggtcct
     5281 ggtcctcaag aacccccgct tcgccctctt cctcctcgtc atcagcggct tcttcttcct
     5341 ctacaaccag gtctacaacg tcatgccgct ctacgtgaag cgggtcgtcg agacgagccc
     5401 ggcgatggac ctctacaccg ccgcgaaccc gttcgtcatc gtcgtcttcc agctcctcgt
     5461 cacgagcctc ttcgggaaga tgaagccggt gaagtcgatg atcgtcggct gcgtgatcat
     5521 cggcgtctcg atggg
//
LOCUS       DAOTEZ010000290         5519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_10581_length_5519_cov_6.418741, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000290 DAOTEZ010000000
VERSION     DAOTEZ010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10581_length_5519_cov_6.418741"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..1094
                     /gene="mqnC"
                     /locus_tag="P5164_14080"
     CDS             18..1094
                     /gene="mqnC"
                     /locus_tag="P5164_14080"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HRY45069.1"
                     /translation="MSGGRLTASEAVLLLEEAPLLELGAAAAAVRRRLHPGGVATYQV
                     DRNINYTNVCIYRCAFCAFYRKAGDEEAYVLPLEEIGAKVEETLALGGTGVLLQGGVH
                     ADLPFSFYEELLSYLRDRYPGIHRHAFSAPEIWFLAKKEKLPVAEVLVRLREAGLMSV
                     PGGGAEILEDGVRKRIVALAKAPTERWAEVHREAHRLGMPTTATMMFGVGETYAERVA
                     HLEVVRRVQDAALDGGRESFTAFIPWTFQEENTELDGKVEVSTGHDYLRTLALSRLFL
                     DNVPHVQGSWVTQGKKIGQVSLFFGADDLGSIMIEENVVAAAGAHNRMTRDDMVALIR
                     DAGFTPRQRGTVYERCSAPCCAAL"
     gene            complement(1126..2199)
                     /locus_tag="P5164_14085"
     CDS             complement(1126..2199)
                     /locus_tag="P5164_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45070.1"
                     /translation="MLSASLALALAAAPASGPPVDVTLPTVHDVRMSVDVAPARDVLL
                     LLGGGPDAPAALRRLRASRPFSVAFSRDGGTPEDLLGRLVSVAAGTPEPLLSGYARRA
                     GTFGKVLDALETEGTPGGLVQARRIAALLPASRPVTARLVLVPLLGLGGFDDVVAEED
                     GETTWLFADLPRLAPEGPAEVVPREAVLSLLRASAAEAWKRLFAPFRQPPGWPGETGP
                     DFGTLLARTVNDGPETLFLIPDEFYPLGTMLEEPIERSFARWNAAAETLLDPKSSADA
                     RREAFLSVTTRGDFWRRHAPVVGAKVSETILRRAGTQRFVEALANGPRAVAELYVEVT
                     RKTKEPQLGKAAKKALEAPPAAR"
     gene            complement(2192..2890)
                     /locus_tag="P5164_14090"
     CDS             complement(2192..2890)
                     /locus_tag="P5164_14090"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HRY45071.1"
                     /translation="MHARPSRLVLFDVDGTLLSSGRRGLTAFGEALRRTFGTEGALAS
                     YRFEGKLDPVIVAELMSGAGVPDDVVAARRPAALELYLDLLEEALAAEPPLLKPGAAE
                     VVARVAATDAVVPALLTGNMPRGARIKLSAAGLWEHFRFGVFGDEAGRRVELGPIALA
                     RALETTGRPFSPSETVIVGDARADVECGRALGARVVAVATGRTSAAELREAGADVVFE
                     TFADVASACEAILA"
     gene            3038..3781
                     /locus_tag="P5164_14095"
     CDS             3038..3781
                     /locus_tag="P5164_14095"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY45072.1"
                     /translation="MAEQKALVGKTVIVTGAGRGIGRAIALSAARAGASVVLASRTEE
                     QLQEVVREVAALGGAAVAVPCDVRRAADVERLVARAVAETGRVDAVVSNAGVFVWKPF
                     EKLTEEEWDTVLDTNLKATYLLVRTALPELKKSHGRIVNVASIHGTAGDANVAAHCAA
                     KFGLVGLTKALALELRPAGIAVNAVCPGSTENRSRSLAPAPHEKPLEEKLDAFDVAES
                     VLFLLSPAAATITGAVLDVWGGTRVEVKA"
     gene            3827..4615
                     /locus_tag="P5164_14100"
     CDS             3827..4615
                     /locus_tag="P5164_14100"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRY45073.1"
                     /translation="MPTSRARVFASTSGGADLADLTGPFLMALLGGAEAEAEEFLRGA
                     RSRGADTALLVRDLVVPALAEVGRMWARGELSVAEEHLATALATRVVARSAGAAAPPA
                     GPGRPRILLACLAGEFHDVGIRLLSDVARESGWDAESLGANVPRESLVRFVAQRRPSA
                     LALSVSLAGHVPEAARTISEAREAAPGMTILVGGRAFCEDADRVPLTGADAGFCDAVA
                     FRDWLRARIGKAKGAGATPPGARAATGLPAELPAALRKRCSRAR"
     gene            4656..4732
                     /locus_tag="P5164_14105"
     tRNA            4656..4732
                     /locus_tag="P5164_14105"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4690..4692,aa:Arg,seq:tct)
     gene            complement(4734..>5519)
                     /locus_tag="P5164_14110"
     CDS             complement(4734..>5519)
                     /locus_tag="P5164_14110"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRY45074.1"
                     /translation="VVETFFLPEEWQKLRAAFDDRETWERHRAKVRTFGPVVADARAP
                     RGTGRRHGAGLKPDGDASHAYRERLRKAIDVLEAVLLTGSRLGEIIGLTWDAVDLKRG
                     RVTIWQPKVGRAKVLPITPELRALLEAQPRGIGKTPVFRRPDPPRRPGQPAPKPAPWD
                     KAKLERAFRLARKLAKIRPELRIHDLRHAAASWATAGGASDRHVRDLLGHTSVSMSAR
                     YSHVRPEDLAGAAGIVSRLASGDAKSDAERPVRKRRSGSKPHE"
BASE COUNT          706 a         1960 c         2144 g          709 t
ORIGIN      
        1 cgctgctcga gcgcgcggtg tcgggcgggc ggctcacggc gtcggaggcg gtcctcctcc
       61 tcgaggaggc gccgctcctc gagctgggcg ccgcggccgc cgcggtgagg cggcgcctcc
      121 acccgggagg cgtcgcgacg taccaggtcg accggaacat caactacacg aacgtctgca
      181 tctaccgctg cgcgttctgc gcgttctacc ggaaggcggg ggacgaggag gcctacgtcc
      241 tcccgctcga ggagatcggc gcgaaggtgg aggagacgct cgcgctcggc gggacggggg
      301 tcctcctgca ggggggcgtc cacgccgacc tgccgttctc gttctacgag gagctcctgt
      361 cgtacctccg ggaccgctat cccggcatcc accggcatgc gttctcggcg cccgagatct
      421 ggttcctcgc gaagaaggag aagctccccg tcgccgaggt cctcgtccgg ctgcgcgagg
      481 cggggctgat gtcggtgccc gggggcgggg cggagatcct cgaggacggc gtgcggaaga
      541 ggatcgtcgc gctcgcgaag gcgccgaccg agcggtgggc cgaggtgcac cgcgaggcgc
      601 accggctcgg gatgccgacg acggcgacga tgatgttcgg ggtgggcgag acgtacgcgg
      661 agcgggtcgc gcacctcgag gtcgtgaggc gcgtgcagga cgccgccctc gacgggggcc
      721 gggagtcgtt cacggcgttc atcccctgga cgttccagga ggagaacacc gagctcgacg
      781 ggaaggtcga ggtctcgacg gggcacgact acctccggac gctcgcgctc tcgcgcctct
      841 tcctcgacaa cgtccctcac gtgcagggct cgtgggtgac gcaggggaag aagatcgggc
      901 aggtgtcgct cttcttcggc gccgacgacc tcggctcgat catgatcgag gagaacgtcg
      961 tcgccgcggc gggcgcgcac aaccggatga cgcgggacga catggtcgcg ctgatccggg
     1021 acgccggctt cacgccgagg cagcgcggca cggtctacga gcgctgctcg gccccctgct
     1081 gcgccgcgct ctgaggggcg ctcccggaga gggcgccgcg cccgctcagc gggcggcggg
     1141 aggcgcctcg agcgcctttt tcgccgcctt cccgagctgc ggctccttcg tcttccgggt
     1201 cacctcgacg tagagctcgg cgacggcgcg cggcccgttc gcgagcgcct cgacgaagcg
     1261 ctgcgtcccc gcccgccgga ggatcgtctc ggacaccttc gcgccgacga ccggagcatg
     1321 gcggcgccag aagtcgccgc gggtcgtcac ggagaggaac gcctcccggc gggcgtcggc
     1381 cgacgacttc gggtcgagga gcgtctcggc ggcggcgttc cagcgggcga agctccgctc
     1441 gatcggctcc tcgagcatcg tgccgagcgg gtagaactcg tcggggatga ggaagagggt
     1501 ctcgggtccg tcgttgacgg tccgcgcgag gagcgtgccg aagtcggggc cggtctcgcc
     1561 cggccagccg ggcggctgac ggaacggggc gaagagccgc ttccaggcct ccgcggccga
     1621 cgcgcggagg agggagagga ccgcctcgcg cgggacgacc tccgccggcc cctcgggggc
     1681 gaggcgcggc aggtcggcga agagccaggt cgtctcgccg tcctcctcgg cgacgacgtc
     1741 gtcgaagccg ccgagcccga ggagcgggac gaggacgagg cgcgcggtga ccgggcgcga
     1801 cgccgggagg agggcggcga tgcgccgcgc ctggacgagc cccccgggcg tcccctccgt
     1861 ctcgagcgcg tcgagcacct tcccgaaggt gccggcccgg cgggcgtagc cggagaggag
     1921 cggctcgggg gtcccggcgg cgacggagac gaggcggccg aggaggtcct ccggggtgcc
     1981 gccgtcgcgg gagaacgcga cggagaacgg gcgggaggcg cgcagccgcc ggagcgccgc
     2041 cggcgcgtcg gggcctcccc cgaggaggag gaggacgtcc cgcgccgggg cgacgtcgac
     2101 cgacatccgg acgtcgtgga ccgtcgggag cgtgacgtcg acgggcggtc cggaggccgg
     2161 cgccgcggcg agggcgaggg cgagcgaggc gctaagcaag gatcgcctcg caggccgagg
     2221 cgacgtcggc gaacgtctcg aagacgacgt cggccccggc ctcgcgcagc tccgcggcgc
     2281 tcgtccggcc ggtggcgacc gcgacgacgc gggcgcccag ggcgcgcccg cactcgacgt
     2341 cggcccgcgc gtcgccgacg atcacggtct cggacgggga gaaggggcgg ccggtcgtct
     2401 cgagcgcgcg ggcgagggcg atcgggccca gctcgacccg tcttcccgcc tcgtccccga
     2461 agacgccgaa gcggaagtgc tcccagaggc cggcggccga gagcttgatc cgggctccgc
     2521 gcggcatgtt cccggtgagg agcgccggga cgacggcgtc cgtcgcggcg acgcgggcga
     2581 cgacctcggc ggcccccggc ttcaggaggg gcggctcggc ggcgagcgcc tcctcgagga
     2641 ggtcgaggta gagctcgagc gcggcggggc ggcgggcggc cacgacgtcg tcggggacgc
     2701 cggcgccgct catgagctcg gcgacgatga ccgggtcgag cttcccctcg aagcggtacg
     2761 aggcgagggc gccttccgtc ccgaacgtcc gccgaagggc ctctccgaac gccgtcaggc
     2821 cgcggcggcc cgaggagagg agcgtgccgt cgacgtcgaa gaggacgagt ctcgaggggc
     2881 gtgcgtgcat cggttcggtc gggcctccgg ggccgattgt aggggtccgg cgggcggagc
     2941 tccccggagc gcggcccctc cgctttcccc ggcaacctgc tagcctcccc gcgcgacaca
     3001 cgcggcccgg aggtgcggac gggcggaggg agcggaagtg gcggaacaga aggcgctggt
     3061 cggaaagacc gtgatcgtca cgggtgcggg ccgcgggatc ggccgcgcca tagcgctctc
     3121 ggccgcccgg gcgggggcgt cggtcgtcct cgcctcgcgg acggaagagc agctccagga
     3181 ggtcgtgcgc gaggtcgccg ctctcggcgg cgcggcggtc gccgtcccct gcgacgtccg
     3241 ccgggccgcc gacgtcgaac ggctcgtcgc acgtgccgtc gcggagacgg gacgcgtcga
     3301 cgccgtcgtc agcaacgccg gcgtcttcgt ctggaagccg ttcgagaagc tgaccgaaga
     3361 ggagtgggac accgtcctcg acacgaacct gaaggcgacc tacctcctcg tccggaccgc
     3421 ccttccggag ctgaagaaga gccacgggcg gatcgtgaac gtcgcgtcga tccacgggac
     3481 ggccggcgac gcgaacgtcg ccgcccactg cgccgcgaag ttcggtctcg tcggcctgac
     3541 gaaggcgctc gctctcgagc tccgcccggc cggcatcgcg gtgaacgccg tctgccccgg
     3601 ctcgacggag aaccggtctc gaagcctggc gcccgcgccg cacgagaagc cgctcgagga
     3661 gaagctcgac gccttcgacg tggccgagtc ggtcctcttc ctcctctccc cggcggcggc
     3721 gacgatcacc ggggccgtcc tcgatgtctg gggggggacc cgagtcgagg tgaaggcgtg
     3781 aggaatccgc cccgccgcgg gcggcgtact cgccttggag aacagagtgc cgacgtctag
     3841 agcaagggtc ttcgcgtcca cctcgggagg ggccgacctc gcggacctca ccggcccgtt
     3901 cctgatggcg ctcctgggcg gggccgaggc cgaggcggag gagttcctcc gcggggcccg
     3961 ctcccgtggc gccgacacgg ccctcctcgt gcgcgacctc gtcgtcccgg cgctcgcgga
     4021 ggtcggccgg atgtgggcgc gcggggagct gtcggtcgcc gaggagcatc tcgcgacggc
     4081 gctcgccacg cgcgtcgtgg cgcgctccgc gggggccgcc gccccgcccg cgggacccgg
     4141 ccggccgcgc atcctcctcg cctgcctcgc cggcgagttc cacgacgtcg gcatccggct
     4201 cctttcggac gtcgcgcgcg aaagcggctg ggacgcggag tcgctcgggg cgaacgtgcc
     4261 gcgggagtcg ctcgttcgct tcgtggcgca gaggcggccc tcggcgctcg ccctctccgt
     4321 gtccctcgcg gggcacgtgc ccgaggccgc gcggacgatc tccgaggcgc gcgaggccgc
     4381 tccagggatg acgatcctcg tcggaggccg cgcgttctgc gaggacgccg atcgggtccc
     4441 tctcaccggg gccgacgccg gcttctgtga cgccgtcgcc ttccgcgact ggctccgggc
     4501 ccggatcggg aaggcgaagg gggcgggggc gacgccgccc ggggcgcgcg cggccacggg
     4561 ccttccggcg gagctgcccg ccgccctccg caagcgctgc tcccgcgcgc gctgagccgc
     4621 cgcctgctac agttcctccc ggtccgctcg agcgcctgcc cgtagctcag ctggatagag
     4681 catcggcctt ctaagccgag ggtcgccggt tcgagcccgg ccgggcaggc cagctattca
     4741 tgcggctttg agccgcttcg gcgcttccgg acgggcctct ccgcgtcact cttcgcgtca
     4801 ccggaggcca gcctcgagac gatcccggcc gctccggcga ggtcctccgg gcggacgtgg
     4861 ctgtagcgcg ccgacatcga taccgacgtg tggccgagga ggtcgcggac gtgccgatcc
     4921 gatgcgcccc cggccgtcgc ccagctcgcc gcggcgtgcc ggaggtcgtg aatcctcagc
     4981 tccggccgga tcttcgccag cttgcgcgcg aggcggaacg cccgctcgag cttcgcctta
     5041 tcccacgggg ccggtttcgg cgccggttgc cccggacggc gggggggatc ggggcgccgg
     5101 aagacgggcg tcttgccgat gccgcggggt tgagcctcga ggagcgcgcg cagctcgggg
     5161 gtgatcggga ggaccttcgc gcgcccgacc ttcggttgcc agatcgtgac ccggccgcgc
     5221 ttcaagtcga ccgcgtccca ggtgaggccg atgatctctc cgagccggct cccggtcagc
     5281 aagacggcct cgaggacatc gatcgccttc ctcagccgct cccgataggc gtgggaagcg
     5341 tccccgtcgg gcttcaggcc ggcaccgtgg cgccgcccgg tccccctcgg cgcgcgggcg
     5401 tcggcgacga cggggccgaa ggtgcggacc ttcgcccgat gccgctccca ggtctcccgg
     5461 tcgtcgaaag ccgcccggag cttctgccac tcctcgggga gaaaaaaggt ctcgacgac
//
LOCUS       DAOTEZ010000291         5513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_10605_length_5513_cov_4.599853, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000291 DAOTEZ010000000
VERSION     DAOTEZ010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10605_length_5513_cov_4.599853"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..1913
                     /locus_tag="P5164_14115"
     CDS             189..1913
                     /locus_tag="P5164_14115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY45075.1"
                     /translation="MPRLDCKRVRGFAAEIAALAVLVIVALAAVRGHEKDDSATADEG
                     IHLFAGAEYVENGTFWMNLEHPPLMKALAGLSLKPLRLTPPAGGRSREGTPHNDYSRF
                     LYFNSVPAHEILRAARRPFPLVFGLLIVVVWATARTLANPGAGLLAAGLIALDPGFVA
                     HASIVHTDVGAALTMTAALVLALAAARRNSLALWAVSGLALGVALATKFSAVLLLPLF
                     AAIALLAFLAEGRLRTARSAAKMALGIGMAGVIALGLLAATYAVNMRAMPAGRPSACV
                     AGFLSGRGAPPDVVDRYARLSLAVPSLGHWVAGIKGVALLSTGERENVNFFRGEISRS
                     GFLTYFPAAFALKSTPAFLLLLAAALALGRRELATYWVGGLLLAASFYFAMSMTSSFN
                     IGVRHLFPVYPLLAISGAVVLAGRLPVRRFAPVAAGLVLSAGVSLASAHPLELGYFNF
                     VLGSSERGAEWFADSNVDWGQDMKRLGDYLRDTGTETKTTIVAYSGLATNYYSRSCQL
                     LDPSRPISPGRYAISDSMQAVGPEFLEGLEGKASADQLRSLLSQLRTRGRRLGRVGAS
                     ITIWELPG"
     gene            complement(1945..2781)
                     /locus_tag="P5164_14120"
     CDS             complement(1945..2781)
                     /locus_tag="P5164_14120"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRY45076.1"
                     /translation="MVGLIRRDAAGCRGRGLRVSGDGAAETEAARVNRAYYEAASAGQ
                     QDYWRLMAAPRARVSTILSLVQVEPVRSLVDLGCGGGGLLREIEARLPGVELAGLDLS
                     SRQIEANRRGGSKIRWAVADLERPMAEDESLSGRWDVVVASEILEHLDEPEAFLGNAL
                     RLATPGRGRLLLTTQSGPVRETERRVGHRQHYTREVLESLLVKTGWTEPRVWNAGFPF
                     HDLSKWWANRDPDASFRRFGDRPYGLSERLVCALLRGAFALNSRSRGAQLYAVGRRGL
                     ER"
     gene            complement(2724..4637)
                     /gene="asnB"
                     /locus_tag="P5164_14125"
     CDS             complement(2724..4637)
                     /gene="asnB"
                     /locus_tag="P5164_14125"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRY45077.1"
                     /translation="MCGIHGILAPRGDLSCALEEMGAHLAHRGPDAAGVERLPGALLG
                     HTRLRVLDLSDAASQPLWDARRSVCIVFNGEIYNYREIRDECLRAGLELRSVSDTEAI
                     AGLFLLEGERAFARLDGIFAFCLFDSRSGDAYLVRDPMGVKPLYFAERPEGLVFASEL
                     GALLASGLVPPIVDPAALQAYLQLDFVPGPMSIVRGVTKLREGHLLHLDGAGRRSIRR
                     YAPPPPPVAGGPGAFAADVAEFDRLVHRTVERQLVADVPVGVFLSGGLDSSIVARTAA
                     EVSPSRISTFSIGFEDPSFDEARWAALVASSIGSDHHVEVFGPRALLDLVPSVAEVLS
                     EPVADGSIFPTLLLSRFARRHVTVALSGDGADELFAGYPTYRAARLARGPSHLPAPVR
                     RALAGAADTLLPVNLDNLSVDFRARKFLAGLDPDPVLRNARWLGTFLPEELPFLLRAH
                     DESLQPAFEELLHEPSADVPRREGLEALLRTDQRFYLQDQVLVKVDRASMSCALEVRV
                     PFLAPEMVAFARALPADRKLRGARGKALLKAWAAGRLPAETVERPKKGFGAPLGRWFR
                     GELRELLEDVLSPTSLARHGLFEPAFVARLLGEHAAGRRDHRKRLFNLLMFQLWWGSF
                     VGTRPAAAGGGCA"
     gene            complement(4661..>5513)
                     /locus_tag="P5164_14130"
     CDS             complement(4661..>5513)
                     /locus_tag="P5164_14130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY45078.1"
                     /translation="GSRDGSWDRIRELARGRPWLRALRFLGNQGQTAAMAAGIEHAQG
                     ELIAFLDADLQNDPRDLPRMLTEILDGRADVVCGWRARRKDRALSRIVPSLVANFLIT
                     HALGLRLHDVGCTLKVFRRVYLENVELYGEMHRFIPAYCAAQGGRIVEVEVHHRPRLH
                     GESKYGLSRVYKVLIDLLTVKMLNTYGSKPAYFFGKLGFVFFGLGTAAFLLVAWRALF
                     RGHPESTPMVFFMLLMYVASLVCVTSGLLAEINIRVLHRVGGKRSYRIIDEVGKRAEA
                     GGTPGVG"
BASE COUNT          844 a         1968 c         1892 g          809 t
ORIGIN      
        1 cgttcgtccc gattcgcacc gcgaaggaga gcgacttccg cccggccacg atgcgggtcc
       61 acgacggagc ggagagctct tcgcgggtcg tcctgcgggt gctgccgggg gagaggccgt
      121 aaggccgaga cgccacaccg ggacggccat cgaggaccca gcctccaacg cctgagtaga
      181 ctcccggaat gccacgactc gactgcaaaa gggtcagagg cttcgcggcc gagatcgcgg
      241 ctctcgccgt tctcgtcatc gtcgccctgg cggccgtcag ggggcacgag aaggacgatt
      301 cggccaccgc agacgaaggc atccatctgt tcgcgggagc cgagtacgtc gagaacggca
      361 ccttctggat gaacctcgag cacccccccc tgatgaaggc tctcgcagga ctctccctga
      421 agccgctccg gctgacccct ccagccggag gcaggtcccg cgaggggact ccgcacaacg
      481 attactcccg cttcctctac ttcaacagtg tccccgcgca cgagatcctt cgggcggccc
      541 ggcgtccctt ccctctcgtc ttcggcctcc tgatcgtcgt cgtctgggcg acggcccgca
      601 ccctcgcgaa ccccggcgcc gggcttctcg ccgcggggct gatcgcgctc gaccccggtt
      661 tcgtcgccca cgccagcatt gtccatacgg acgtcggcgc cgcgctcacg atgacggcag
      721 ctctcgtcct tgcgctcgcg gcggcaaggc gcaactccct ggccctctgg gccgtttccg
      781 gcctcgccct cggggtcgcc ctcgcgacga agttctcggc ggtacttctg cttccgctct
      841 tcgccgcgat agcgctcctc gcgttcctcg ccgaaggaag gctccggacg gcccgttccg
      901 cagcgaagat ggccctcggc atcggaatgg cgggcgtgat cgccctcggg ctcctcgccg
      961 cgacctacgc cgtcaacatg agggcgatgc cggcagggag gccctccgcc tgcgtcgcgg
     1021 ggtttctttc cggccgcgga gcaccacccg acgtcgtcga ccggtatgcg cgactctctc
     1081 tcgccgtgcc ttcgctcggg cactgggtcg ccgggatcaa gggcgtcgcg ctcctgagca
     1141 cgggggaacg cgagaacgtc aacttcttcc gcggcgagat ctcgcgctcc gggttcctga
     1201 cctacttccc cgccgccttc gccctgaagt cgacgcctgc cttccttctc cttctcgccg
     1261 ccgcactcgc actcggacgc cgcgagctcg cgacctactg ggtcggcggt ctcctcctcg
     1321 ccgcgtcttt ctacttcgcg atgtcgatga cgtcgtcctt caacatcggc gtccgccacc
     1381 tcttcccggt ctacccgctt ctcgcgatat ccggggccgt cgtcctggcc ggtcggctac
     1441 cggtccgccg tttcgcgccg gtggcagccg gcctcgtcct ctcggcgggg gtctcgctcg
     1501 catcagccca cccgctcgag ctcggctact tcaatttcgt cctcggcagc tccgagcggg
     1561 gcgcggagtg gttcgccgac tcgaacgtcg actgggggca ggacatgaag cgcctcggcg
     1621 actacctccg tgacaccggc acggagacga agacgaccat cgtcgcctac agcggtctcg
     1681 ccacgaacta ctactcgcgt tcttgccagc tcctcgatcc ctcgaggccc atctccccgg
     1741 gccgctatgc gatctcggac tcgatgcagg cggtcggacc ggagttcctc gaggggttgg
     1801 aggggaaagc ctcggcggat cagctccgtt cgctgctctc ccagcttcgg acacgaggaa
     1861 ggcggctggg gcgcgtcggt gcttcgatca cgatctggga gcttccgggc tgacgtcggt
     1921 ccgccttcgc acgcgccccg gccgtcagcg ctcgagtccc cttcgtccga ctgcgtagag
     1981 ctgcgctccc cgcgaacggg agttcagcgc aaacgcgccc ctgaggaggg cgcagaccag
     2041 cctttcgctc aggccatacg gtcggtcgcc gaatcggcgg aacgaggcgt cgggatcccg
     2101 gttcgcccac catttcgaga gatcgtgaaa cggaaatccc gcgttccaca cgcgtggctc
     2161 cgtccagcct gtcttcacga ggagcgattc caggacctct cgcgtgtagt gctggcggtg
     2221 accgacgcgg cgctcggtct cccggaccgg accgctctgg gtcgtcagaa ggaggcggcc
     2281 tcttcccggc gtcgcgagcc ggagagcgtt ccccaggaag gcctccggct catccaggtg
     2341 ctcgaggatc tcggacgcga cgacgacatc ccaccgtccg gagagactct catcctcggc
     2401 catgggcctc tccaggtccg caaccgccca gcggatcttc gacccgccac gccggttcgc
     2461 ctcaatctgc cggctcgaaa ggtcgaggcc tgcgagttcg acaccgggga ggcgggcctc
     2521 gatctctctc aggagccccc ctccgccgca gccgaggtcg acgagcgatc ggaccggctc
     2581 cacctgcacg agggacagga tcgtggagac ccgagcccgc ggcgccgcca tgagacgcca
     2641 gtagtcctgc tggccggcgg acgcggcctc gtagtaggca cggttcaccc gcgccgcctc
     2701 ggtctcggcc gcgccgtccc cgctcacgcg cagccccctc ccgcggcagc cggccgcgtc
     2761 ccgacgaatg agccccacca tagctggaac atcaggaggt tgaagaggcg cttgcggtga
     2821 tcgcgacgcc ctgccgcgtg ctccccgagg agtctcgcca cgaaggccgg ctcgaaaaga
     2881 ccgtggcgcg cgagggaggt cggagagagg acgtcctcga ggagctcgcg gagctccccg
     2941 cggaaccacc tgccgagcgg ggcgccgaag cccttcttcg gccgctccac ggtctcggcg
     3001 gggaggcggc ccgccgccca cgccttgaga agggcctttc cccgcgcgcc gcgcagcttc
     3061 cggtcggcag ggagagcacg cgcgaacgcg accatctcgg gcgcgaggaa ggggacccgc
     3121 acctcgagcg cgcacgacat gctcgcccgg tcgaccttca cgaggacctg gtcctgcagg
     3181 tagaagcgct ggtcggtgcg gagaagcgcc tcgagcccct ctcggcgcgg gacgtccgcc
     3241 gacggctcgt gcaggagctc ctcgaacgcc ggctggaggc tttcgtcgtg cgcacggagg
     3301 agaaacggga gctcctccgg caggaaggtc ccgagccagc gtgcatttct gaggacggga
     3361 tcggggtcga ggcccgcgag gaactttcgg gcccggaagt cgaccgagag gttgtcgagg
     3421 ttcaccggga ggagggtgtc cgccgccccc gcgagcgccc gccggacggg cgccgggaga
     3481 tgcgacggtc cgcgcgcgag cctcgcggcg cggtaggtcg ggtagccggc gaagagctcg
     3541 tccgccccgt ccccggagag cgccacggtg acgtgccgcc gcgcgaacct cgagaggagg
     3601 agcgtcggga agatcgaccc gtcggccacg ggctcggaaa ggacctcggc gaccgacggc
     3661 acgaggtcga gcagcgcgcg gggcccgaag acttcgacgt ggtggtcgct gccgatcgac
     3721 gaggcgacga gggcggccca gcgggcctcg tcgaaggacg ggtcctcgaa cccgatcgag
     3781 aaggtcgaga tccgcgacgg cgagacctcg gccgccgtcc gggcgacgat cgaggagtcg
     3841 aggcctccgg agaggaagac gccgacgggg acgtcggcca cgagctgccg ttccaccgtc
     3901 cggtggacga ggcggtcgaa ctcggcaacg tcggccgcga aggccccggg ccctcccgcg
     3961 acgggcggcg gcggcggcgc gtagcgccgg atcgaccgcc ggcccgcgcc gtccaggtgg
     4021 aggagatgcc cctcgcggag cttcgtgacg ccgcggacga tcgacatcgg gcccggcacg
     4081 aagtcgagct ggaggtaggc ctggagcgcg gccgggtcga cgatgggagg cacgaggccg
     4141 ctggcgagaa gggcgccgag ctccgacgcg aagacgaggc cctcgggccg ctccgcaaag
     4201 tagaggggct tgacgcccat cgggtcccgg acgaggtacg cgtcgccgct gcgggaatcg
     4261 aagaggcaga acgcgaagat gccgtcgagc cgcgcgaagg cccgctcgcc ctcgaggagg
     4321 aacaggccgg cgatcgcctc ggtgtccgag accgagcgga gctccaggcc ggcccggagg
     4381 cactcgtccc gaatctcccg gtagttgtag atctcgccgt tgaagacgat gcaaacggag
     4441 cgccgggcgt cccagagcgg ctgggacgcg gcgtccgaca ggtcgaggac ccgaagcctc
     4501 gtgtgcccca ggagggcccc cggaagccgc tcgacccccg cggcgtccgg accccggtgg
     4561 gcgaggtgcg cgcccatctc ctcgagcgcg caagagaggt cgccgcgtgg ggcgaggatg
     4621 ccgtggatgc cgcacatgaa cgcccctgca ccgtcccgga ctatccgacc ccgggcgtcc
     4681 cgccggcctc cgcgcgcttt ccgacctcgt cgatgatccg gtagctcctc ttcccgccga
     4741 ccctgtgcag gacccggatg ttgatctcgg ccaggaggcc cgacgtgacg cagacgaggg
     4801 aagccacgta catcaggagc atgaagaaga ccatcggggt gctctcgggg tggccgcgga
     4861 agagcgccct ccaggcgacg agcaggaacg ccgcggtgcc aagaccgaag aagacgaacc
     4921 cgagcttgcc gaagaagtag gccggcttgg agccgtaggt gttgagcatc ttgacggtga
     4981 ggaggtcgat caggaccttg tagaccctcg aaaggccgta cttgctctct ccgtggagcc
     5041 ggggccggtg gtggacctcc acctcgacga tccgcccacc ctgcgccgcg cagtacgcgg
     5101 ggatgaaccg gtgcatctcg ccgtagagct cgacgttctc caggtagacg cggcgaaaca
     5161 ccttgagcgt gcagccgacg tcgtggaggc ggaggccgag cgcgtgcgta atgaggaagt
     5221 tcgcgacgag cgaggggacg atccgcgaca acgcccggtc cttccggcgt gctctccagc
     5281 cgcagacgac gtccgcccgg ccgtcgagga tctcggtgag catccgggga aggtcacgcg
     5341 ggtcgttctg cagatccgcg tccaggaacg cgatcagctc tccctgcgcg tgctcgattc
     5401 cggccgccat cgccgcggtc tggccctggt ttccgaggaa ccggagggcc cgcagccacg
     5461 gccggcctct cgccagctcg cgaatccgat cccagctgcc atcccggcta ccg
//
LOCUS       DAOTEZ010000292         5499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_10649_length_5499_cov_6.122704, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000292 DAOTEZ010000000
VERSION     DAOTEZ010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10649_length_5499_cov_6.122704"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1081)
                     /locus_tag="P5164_14135"
     CDS             complement(<1..>1081)
                     /locus_tag="P5164_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRY45079.1"
                     /translation="REAESVAAVRELLHVAYWFARTYGRATKPAPGITFDAAALPKTA
                     PLPRQTVDQLRALAASLKEKDEKLADLLAEKATVDDELQRLRAEIAEVAAAKKANAAR
                     PDPHDYSEAETRDFFIDLLLQEAGWPLDEARDREFPIEGMPNAEGVGYADYVLWGDDG
                     RPLAVIEAKRTRKSPQAGQQQAKLYADGLEARFGQRPVIFFTNGYEHWIWDDAAYPPR
                     RAAGFLKKGELELTIQRRTSRKPLAGAEVNRRIVERHYQTRAIRRVCEAFERDRERKA
                     LLVMATGAGKTRTVIALCDLLMRCGWVKRVLFLADRVALVNQAVGAFKTHLPDSPPVN
                     LVTEKGGEGRVYVSTYPTMMGLIDET"
     assembly_gap    1082..1181
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1372..2442)
                     /locus_tag="P5164_14140"
     CDS             complement(1372..2442)
                     /locus_tag="P5164_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRY45080.1"
                     /translation="MSAFAPRFSISNAAAASLTLIERARGFLDAANLSSEWVARMSQR
                     ALLLEAHATTHIEGTELTLEQAERLWTGQAVEEAKPDDVRELLNYREAFAFVSGYVAS
                     GEPITEGLVREIHRRLVEGVRGGEGSPGAYRTIQNHVVNSATREVVYIPPPPGDVAPL
                     MRELVEWLRSESGVHPVLAAGIAQFQLVHIHPFVDGNGRTSRLLSTLCLYRAGYDFKR
                     LFTLSEFYDRDRSRFYRALQGVRASGMDLSGWLDFFTEGLATQLGEVKARGEVVIRAD
                     LASRAHHLNSRQGAVLEEVLLRGGATLGEVEGRFPGVSRRTIQRDFRFLIGKGLLEEE
                     GKPTDPNRRYLRGKALASYDKL"
     gene            complement(2591..3298)
                     /locus_tag="P5164_14145"
     CDS             complement(2591..3298)
                     /locus_tag="P5164_14145"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HRY45081.1"
                     /translation="MTRRKDIYRGEDPRFLPFYGLREAALCLRLPVATLRQWTMGREY
                     EIKSGRKVFLSLIRSADPERNVLSFVNLLEGQALRAFRREHKLGFKVLRKALASLKVL
                     DGPRAKKHPLAFEDFLTDGLDILVERVGELIGLNPARQIAIRAAFRKHLQRIRRDSTG
                     PTAFFPWVGGEGETTMPVAVDPRVAFGRPVLTGTGIATAVIASFVRAGERIEDIAEDY
                     DLSPEQVETAVTFEEAS"
     gene            complement(3389..4591)
                     /locus_tag="P5164_14150"
     CDS             complement(3389..4591)
                     /locus_tag="P5164_14150"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HRY45082.1"
                     /translation="MRAPLARVGDLAEQIRGVTYAKGDASDEPRDGYVPILRAGNISD
                     EGLSLSDLVFVPAARVSDRQFLKRNDVLVAASSGSLDVVGKAALVNSDVKAGFGAFCK
                     VLRPGERVHPAYFAHFFKTPAYRGRVSALAAGANINNLRNEDLDDLTLPLPSAEEQHR
                     IAGVLDRAEALRAKRRAALAQSDGLTQAIFLEMFGDPVSNPLKWPEHEMEALIDGGPQ
                     NGLYKPASEYGSGVPILRIDAFYDGVVTDLHSLKRVRITEPELALYALRTGDIVVNRV
                     NSIEYLGKSALIPLLPEPIVFESNMMRISLRRDLVDSRWVIEFLQSPFIRGQLLNRAK
                     RAVNQASVNQQDVKSLRLLAPPLHLQRSFAARAAAVDGLKSSHRASLAQLDALFASLQ
                     HRAFRGEV"
     gene            complement(4588..>5499)
                     /locus_tag="P5164_14155"
     CDS             complement(4588..>5499)
                     /locus_tag="P5164_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HRY45083.1"
                     /translation="ERHPELFRDAKLKKHFHEAAFHGFDFDNTMLRIGSMNMLLHGVE
                     SPAIRYRDSLAQDHAGEEERYTLVLANPPFAGSLDYENTAKDLQQVVRTKKTELLFLA
                     LFLRLLKPGGRAAVIVPDGVLFGSSTAHRTLRKILVEEQKLDAVVSLPGGVFKPYAGV
                     STAILLFTKTNSGGTDHVWFYDCDADGWSLDDKRTPLLPDEKLGPVPSEPLSPADHAK
                     NNLPDILARWPERAGKERKRPRTAQSFTVAKADIVAQSYDLSINRYKEVVHEEVAHKA
                     PKEILAELRKLEKEIEEGMKELEGMVG"
BASE COUNT          801 a         1789 c         1891 g          918 t
ORIGIN      
        1 tcgtctcgtc gatgagcccc atcatcgtcg ggtaggtcga gacgtagacg cgcccctcgc
       61 cgcccttctc cgtgacgagg ttcaccgggg gcgagtcggg gagatgcgtc ttgaacgcgc
      121 cgaccgcctg gtttacgagc gcgacccggt cggcgaggaa gaggacgcgc ttcacccagc
      181 cgcagcgcat caggaggtcg cagagggcga tgaccgttcg cgtcttcccg gcgcccgtcg
      241 ccatgacgag gagcgccttt cgctcccggt cgcgctcgaa cgcctcgcag acgcggcgga
      301 tcgcccgcgt ctggtagtgg cgctcgacga tccgccggtt cacctccgcc ccggcgagcg
      361 gctttcggct cgtccgccgc tggatcgtca gctccagctc gcctttcttc aggaagcccg
      421 ccgcgcggcg cggcgggtag gcggcgtcgt cccagatcca gtgctcgtag ccgttcgtga
      481 agaagatgac ggggcgctgt ccgaagcgcg cttcgaggcc gtcggcgtag agcttcgcct
      541 gctgctggcc ggcctgcggc gactttcgcg tccgcttcgc ctcgatgacg gcgagcgggc
      601 gcccgtcgtc gccccagagg acgtagtcgg cgtagccgac cccttcggca ttcggcatcc
      661 cttcgatcgg gaactcccgg tcgcgcgcct cgtcgagcgg ccagcccgcc tcctggagga
      721 gaaggtcgat gaagaagtcg cgcgtctcgg cctcggagta gtcgtgcggg tcgggccgcg
      781 ccgcgttcgc cttcttggct gcggcaactt cggcgatctc ggcgcgcagg cgctggagct
      841 cgtcgtcgac cgtcgccttc tcggcgagca ggtccgcgag cttctcgtcc ttctccttca
      901 gcgaggcggc aagcgcgcgc agctggtcga ccgtctgccg cgggagaggc gccgtcttcg
      961 ggagcgccgc cgcgtcgaac gtgattccgg gcgcgggctt cgtggctcgc ccgtacgtcc
     1021 tcgcgaacca gtacgccacg tggagcagct cgcggacggc cgcgaccgac tcggcctcgc
     1081 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngggtgcgtc acgcggggcc
     1201 gcccgtcatc tcgccgtgcc ttgttcaagc gtcgctatca caagggagcc ggccgaccgc
     1261 atgttcaagg ctggcgagcg catagcgtcc gccgcgttcg gccgaggccg gctcggcgga
     1321 ctcgcggttc ccgtgtcgca gctccgtgtc acagcctgtc acagctccgt gtcacagctt
     1381 gtcatagctc gctaaagcct tgccacggag atatctgcga ttcggatccg tgggctttcc
     1441 ctcctcctcc agaagaccct tcccgatcag gaagcggaaa tccctctgga tcgtcctgcg
     1501 gctcacgccc gggaatcgcc cttcgacctc gcccagcgtg gcgccgcccc ggaggaggac
     1561 ctcttccaga accgctccct gccgcgaatt caggtggtgc gcgcgggagg cgagatcggc
     1621 ccggatgacg acctcgcccc gggccttcac ctcgccgagc tgcgtcgcca gcccctccgt
     1681 gaagaagtcg agccagccgg aaaggtccat cccgctcgcg cgcacgccct ggagcgcccg
     1741 gtagaagcgg ctccgatcgc ggtcgtagaa ctcgctgagc gtgaagaggc gcttgaagtc
     1801 atagccggcc cggtagaggc agagcgtcga gaggagccgc gacgtccggc cgttgccgtc
     1861 gacgaacggg tggatgtgga cgagctggaa ctgggcgatc ccggcggcga ggaccgggtg
     1921 gacgcccgat tcgctccgga gccactccac cagctcccgc atcaggggcg ccacgtcgcc
     1981 gggcggcggc gggatgtaga cgacctccct cgtcgcgctg ttcacgacgt ggttctggat
     2041 cgtccgatac gccccgggcg agccctcccc gccgcggacc ccctcgacga gccgccggtg
     2101 gatctcgcgg acgagcccct ccgtgatcgg ctcgccgctc gcgacgtatc ccgacacgaa
     2161 cgcgaacgcc tcgcggtagt tgaggagctc ccggacgtcg tccggcttcg cctcctcgac
     2221 cgcctggccc gtccagagcc gctcggcctg ctcgagcgtc agctccgtcc cctcgatgtg
     2281 cgtcgtcgcg tgggcctcga ggaggagcgc ccgctggctc atccgtgcga cccattcgga
     2341 cgagaggttc gcggcgtcga ggaagccccg cgcgcgctcg atcagcgtga gcgacgcggc
     2401 ggccgcgttc gagatcgaga agcgaggcgc gaaggcgctc atgtcgaggg ctcctgtgct
     2461 cgacggattc tcgcggcccg agcgtgatct gtcgatcctc tgaccttctc aggttcgagg
     2521 tcgcgtccgg gctcggccgt tgtgaagctc ggggccggag aggtcctcgt cgaggaagag
     2581 ggcggcgagc ctaggacgcc tcctcgaacg tgacggctgt ttcgacctgc tccggggaga
     2641 gatcgtagtc ctccgcgatg tcctcgatcc gctcgcccgc cctcacgaaa ctcgcgatga
     2701 cggccgtggc gattcccgtg ccggtgagga cgggccggcc gaaggcgacc cgaggatcga
     2761 ccgcgaccgg catcgtcgtc tcgccctcgc cgccgaccca ggggaagaac gcagtggggc
     2821 cggtggagtc gcgccggatt ctctggaggt gtttccggaa ggctgcccgg atggcgattt
     2881 ggcgcgcggg attgagcccg atcagctcgc cgacccgctc gacgaggatg tcgaggccgt
     2941 ccgtcaggaa gtcctcgaac gccagcgggt gcttcttcgc gcgtggcccg tcgaggacct
     3001 tcagcgacgc gagagctttt cggaggacct tgaaccccag tttgtgttcc cggcggaacg
     3061 cccgcagcgc ctgaccctcg aggaggttca cgaaggagag gacgttgcgc tcgggatctg
     3121 cggagcggat cagcgacagg aagaccttcc gtccggactt gatctcgtac tcccggccca
     3181 tcgtccactg cctcagtgtg gcaaccggga ggcggaggca gagcgccgcc tcccgtagac
     3241 cgtagaacgg caggaagcgc gggtcctcgc cgcggtagat gtctttccga cgcgtcaaac
     3301 cggggggcct cctggggaat tgtccctgat ggcgaccggc ccgggcggag tggcgctgaa
     3361 ggcggcaccc gggccgcttg tcggggccct acacctcgcc gcggaaggcg cgatgctgga
     3421 gcgaggcgaa gagggcgtcg agctgggcga gcgaggctcg gtgggaggac ttgagcccgt
     3481 cgacggcggc ggcgcgggcg gcgaaggagc gttgcaggtg aagcggcggc gcaagaagcc
     3541 gcagagactt gacgtcctgt tgattgacgc tcgcctgatt cacggcccgc ttcgcgcggt
     3601 tgagaagctg gcctcggatg aacgggctct ggaggaactc gatcacccag cgcgagtcca
     3661 cgagatcgcg ccgaagggag atccgcatca tgttcgattc gaagacgatc ggctcgggga
     3721 gaagcggtat gagcgcgctc ttgccgagat actcgatgct gttcacgcgg ttcacgacga
     3781 tgtcgccggt gcgaagggca taaagagcca gctcgggctc ggtgattcgg actctcttca
     3841 gcgagtgcag atccgtcacg accccgtcgt agaaggcgtc gatcctaagg atcggtacac
     3901 cagagccgta ctcagaagcc ggcttgtaca ggccgttctg cggcccgccg tctatgaggg
     3961 cttccatctc gtgttcgggc cacttcaacg gatttgacac cggatccccg aacatctcga
     4021 ggaagatcgc ctgcgtcagg ccgtcgctct gcgcgagggc cgcccgccgc ttcgcccgca
     4081 gagcctcggc ccgatcgagg acccccgcga tgcggtgctg ctcctcagca gacggcagcg
     4141 ggagagtgag gtcgtcgagg tcctcgttcc tcagattgtt gatgttcgcc ccggccgcga
     4201 gagccgatac tcgaccccga tacgccggcg tcttgaagaa gtgcgcgaag tacgccggat
     4261 ggaccctctc gccaggtcgc agcaccttgc agaaggcccc gaagccagcc ttgacatcgg
     4321 agttgacgag agctgccttc cccacgacgt cgaggcttcc gctcgacgcg gcgacgagga
     4381 catcgttccg cttgaggaac tgtcgatcgg acacgcgcgc tgccggaacg aacacgaggt
     4441 cggagaggct cagcccttcg tcggagatgt ttccggcgcg aaggatggga acgtacccgt
     4501 ccctgggttc atcggaggcg tcgcctttcg cgtaggtgac ccctcgaatc tgctctgcga
     4561 gatctccgac acgcgcgagt ggcgccctca cccgaccatc ccctccagct ccttcatccc
     4621 ctcctcgatc tccttctcca gcttccgcag ctccgcgagg atctccttcg gcgccttgtg
     4681 cgcgacctcc tcgtggacga cctccttgta gcggttgatc gataggtcgt agctctgggc
     4741 gacgatgtcg gccttggcga cggtgaagct ctgggcggtg cggggccgct tgcgctcctt
     4801 gccggcgcgc tcgggccagc gggcgaggat gtcggggagg ttgttcttcg cgtggtcggc
     4861 gggggagagc ggctccgacg gcacggggcc gagcttctcg tcggggagaa ggggcgtccg
     4921 cttgtcgtcg agcgaccagc cgtcggcgtc gcagtcgtag aaccagacgt ggtcggtccc
     4981 gccggagttc gtcttcgtga agaggaggat ggcggtcgag acgcccgcgt agggtttgaa
     5041 gacaccgccg gggagggaga cgacggcgtc tagcttctgc tcctcgacga ggatcttccg
     5101 gagcgtccgg tgggcggtgc tcgacccgaa gaggacgccg tcggggacga tgacggcggc
     5161 gcggccgccg ggcttcagga gccggaggaa gagggcgagg aagaggagct cggtcttctt
     5221 cgtcctcacg acctgctgga ggtccttcgc ggtgttctcg tagtcgaggg agcctgcgaa
     5281 cggagggttc gcgaggacga gggtgtagcg ctcctcctcg cccgcgtggt cctgcgccag
     5341 cgagtcgcgg tagcggatgg cggggctctc cacgccgtgg aggagcatgt tcatgctccc
     5401 gatccggagc atcgtgttgt cgaagtcgaa gccgtggaag gcggcctcgt ggaagtgctt
     5461 cttaagcttc gcgtcgcgga agagctcggg atgccgctc
//
LOCUS       DAOTEZ010000293         5488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_10688_length_5488_cov_5.885146, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000293 DAOTEZ010000000
VERSION     DAOTEZ010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10688_length_5488_cov_5.885146"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /locus_tag="P5164_14160"
     CDS             <1..1226
                     /locus_tag="P5164_14160"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRY45084.1"
                     /translation="LLPHLLLAPPAAAGLAEPFPGSPPRLQLPVELLVPGASAVAIAT
                     LGLVALLFWARDRSRTDLLLYTLLASGLATFLVLGLRSAPSGDSPAAAALGLLLPALA
                     WLLLVRFLDLPLRRGRLSLLALPAVALPFVVALPSARALAAPMEAVALVVFGGAALLD
                     LVRLHRPDAALLFAATAVLLLAALFDAAAAKGVLLPGERLPPLLGPAFLLFTALLLVA
                     VADQGRRLVEKATIDALTGLPNRATFLERARRELDRAQRNGSSVALAMLDVDHFKRFN
                     DRYGHPAGDRVLKDAGRAVASAVRGIDLAGRYGGEELVLLLVDVDAEAALAAVERVRA
                     AIAEVRLPHVPDAVTASAGVALHHGAFDRCGVADLIRRADAALYRSKAEGRDRTTLET
                     RGPERPTSPAEVRYR"
     gene            1259..1738
                     /locus_tag="P5164_14165"
     CDS             1259..1738
                     /locus_tag="P5164_14165"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRY45085.1"
                     /translation="MGGDTGIRDVVTGLYVREYFDDVIARELERSRRHGIHLSVLSVV
                     LANLAELSEAGEETALAAVVEAARALQAIVRETDHLFRWEEDELLLLLFNADAEACAL
                     KVSQLDQLFRPWRDGGGPVACAVKVRVGGATHEEHLVFASVLQAARETARGGASGPA"
     gene            complement(1752..2579)
                     /gene="mazG"
                     /locus_tag="P5164_14170"
     CDS             complement(1752..2579)
                     /gene="mazG"
                     /locus_tag="P5164_14170"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRY45086.1"
                     /translation="MPPEPRSPRPEAAGEAAAALVRLMARLRAECPWDRKQTFADLSG
                     YLLEECYEALDALARGNLHELEGELGDVLFQIVFLAELGRESNAFDLASVAERIHAKM
                     VSRHPHVFGEAEVADAEAVKVQWEKLKEQERSPAGREEPLSPFAGVPAALPALLKAVR
                     VTSRAADLGFDWERDADVLAKLDEEVAEFKVELLAEGATRESVAREIGDILFTVVNVA
                     RRHGVDPEAALQTTNAKFVRRFEEVSRRVRASGRDVRDAPLEELDRTWDEVKRDEGP"
     gene            2646..3107
                     /locus_tag="P5164_14175"
     CDS             2646..3107
                     /locus_tag="P5164_14175"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HRY45087.1"
                     /translation="MSEAPKTIKVVDRRMFTAEGDLRDDVKEELAATPPAPEPPPPAP
                     ETAAPPAPEQTTPTSPAFLRLLDMLAQTASLYLQGVPDPATGRRAVDLNASREIIDTL
                     LALREKTRGRLSFEETDALEGLLGELQLVYSRLATQARGGGAMPPPPRPKG"
     gene            3122..5026
                     /locus_tag="P5164_14180"
     CDS             3122..5026
                     /locus_tag="P5164_14180"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY45088.1"
                     /translation="MRRRTGLGPSRPGALLAALALLFPLLLSGLPASAQVLQAPGEAT
                     ESTFRREVRGQWFAWLAAHEEGDPLLAAQKVEEIVKHARKIGLKRLTDLSLAATLLAR
                     REVTQGSPEKARWALDAAVRLDPDLPEARWARVGLALKADLLAAPRELLGAIRASVVD
                     LEGRRVLYVRTSLLLVLTLAAVGLSFVVLLVAGTSRRLFHDLTELAGRWVPRSAEPVV
                     ALALFLLPLLLALDVGWLLLWLFVLSLGYAERNVRWAAVAGLVPLLFVAPALDRAAVS
                     LSSSASPVLRGAEALEEKRYDQRVLDDLEAVKALFPEDADIRFLLGCLYQQLGQNDRA
                     VAEYTVASQASTAEVRALINRGDIRFVDGDFGAAQEDFQEALRRDPRDVRARYNLSLV
                     YGETFRTVEAQEKLSEARALDNALVTRFLDSPTLVKVVSLGFTPDEARAKIALLQRDP
                     RSRRILGHFHLGSVVATWAVPLAVAIPFALVAAFLLDSFRRKGYGYALACQKCGRTFC
                     RLCKPPGESPLLCSQCVHVYLKKDGVSIETKLQKVEEVKRRQGFEGRLRRVLNLLLPG
                     GEPFLDGRVAKAAAILAVFFFALLAALFREHLALSPRPGAERLLAGTLLWGLVAAGAW
                     VSGQLAARKG"
     gene            5031..>5488
                     /locus_tag="P5164_14185"
     CDS             5031..>5488
                     /locus_tag="P5164_14185"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HRY45089.1"
                     /translation="MALEGTLHDFALADILQLIALQKKTGLLTLRGPADTVILGFDAG
                     LLVSAESQARRIDTRLGTLLVKTRSLSPESLARALEIQGQTLQRLGFILLRNGFCDAD
                     QLRAGLDAQVRKIAYGLFRWTDGDYVFDPQEKIDYDREYVHPIQVESLLM"
BASE COUNT          665 a         2085 c         1920 g          818 t
ORIGIN      
        1 cgttgctccc gcacctcctc ctcgcgccgc cggccgctgc cggtctcgcg gagccgttcc
       61 cgggttcccc gccccggctc cagctccctg tggagctcct cgtgccgggg gcttcggccg
      121 tcgcgatcgc gacgctcggc ctcgtcgcgc tcctcttctg ggcgcgcgac cggagccgga
      181 cggaccttct cctctacacg ctcctcgctt cggggctcgc gacgttcctg gtcctcggcc
      241 tgcggtccgc cccgtccgga gactccccgg ccgcggccgc gctcggcctc ctcctccccg
      301 cgctcgcctg gctcctcctc gtccggttcc tcgaccttcc gcttcggcgc ggtcgtctct
      361 cgctcctcgc cctcccggcc gtggcgctcc cgttcgtcgt ggcgcttccc tccgcccggg
      421 cgctcgcggc gccgatggag gcggtcgcgc tcgtcgtctt cgggggggcg gcgctgctcg
      481 acctcgtccg cctccaccgg cccgacgcgg ccctcctctt cgcggcgacc gccgtcctcc
      541 tcctggccgc gctgttcgac gcggccgcgg cgaagggcgt cctccttccc ggggagcggc
      601 tcccgcccct gctcgggccg gcgttcctcc tcttcacggc gctcctcctc gtcgcggtgg
      661 cggaccaggg gagacgcctg gtcgagaagg cgacgatcga cgcgctgacg gggctcccga
      721 accgggcgac gttcctcgag cgggctcgtc gggagctcga tcgggcgcag cggaacggga
      781 gctccgtggc gctcgcgatg ctcgatgtcg accatttcaa gcgcttcaac gaccgctacg
      841 ggcacccggc cggagaccgg gtcctgaagg acgcgggacg cgccgtcgcc agcgccgtcc
      901 gcgggatcga cctcgccggg cgctacggcg gcgaagagct cgtcctgctc ctcgtcgacg
      961 tcgacgcgga ggcggcgctc gccgccgtcg agcgggtccg ggcggcgatc gcggaggtcc
     1021 gtctcccgca cgtccccgac gcggtcacgg cgtcggcggg cgtcgcgctc caccacggcg
     1081 cgttcgaccg gtgcggggtg gccgacctga tccgccgggc cgacgcggcc ctctaccgga
     1141 gcaaggccga ggggcgcgac cggacgacgc tcgagacgcg cggaccggag cggccgacgt
     1201 ctcccgccga ggtccgctac cgctgaggcg gcttccgcgc tggtaagatc gccgggcgat
     1261 gggcggcgac acggggatcc gggacgtcgt gacaggcctt tacgtacggg agtacttcga
     1321 cgacgtcatc gcgcgggagc tcgagcggtc gcgccgccac gggatccacc tctccgttct
     1381 ctccgtggtc ctcgcgaacc tggcggagct gtcggaggcg ggggaggaga cggcgctcgc
     1441 cgccgtcgtc gaggcggcgc gcgccctcca ggccatcgtc cgggagacgg accacctctt
     1501 ccggtgggag gaggacgagc tcctcctgct cctcttcaac gccgacgccg aggcctgcgc
     1561 gctgaaggtg agccagctcg accagctctt ccgtccgtgg cgcgacggcg gaggcccggt
     1621 cgcatgcgcg gtcaaggtcc gcgtcggcgg tgcgacgcac gaggagcacc tggtctttgc
     1681 gagcgtgctg caggccgcgc gcgagacggc gcgcggcggg gcgtccggcc ccgcctgacc
     1741 ggtccgcccc gtcaggggcc ctcgtcccgc ttcacctcgt cccaggtccg gtcgagctcc
     1801 tcgagagggg cgtcgcgcac gtcccggccg gaggctctca cgcggcggga gacctcctcg
     1861 aaacgcctca cgaacttcgc gttcgtcgtc tggagggcgg cctccgggtc gacgccgtgc
     1921 cgccgcgcga cgttgaccac ggtgaagagg atgtccccga tctcccgagc gaccgattcg
     1981 cgcgtggcgc cctcggcgag gagctcgacc ttgaactcgg cgacctcctc gtcgagcttc
     2041 gcgagcacgt cggcgtcgcg ctcccagtcg aacccgaggt ccgccgcgcg ggacgtgacg
     2101 cggaccgcct tgaggagggc ggggagggcg gccgggacgc cggcgaacgg agagaggggc
     2161 tcctcgcgcc cggccgggga gcgctcctgc tccttcagct tctcccactg gaccttcacg
     2221 gcctccgcgt cggcgacctc ggcctcgccg aagacgtggg ggtgccggga aaccatcttg
     2281 gcgtggatgc gctcggcgac cgaggcgagg tcgaaggcgt tcgattcccg ccccagctcg
     2341 gcgaggaaga cgatctggaa gaggacgtcg ccgagctctc cttcgagctc gtgaaggttg
     2401 ccccgcgcga gggcgtcgag ggcctcgtag cactcctcga ggaggtaccc cgagaggtct
     2461 gcgaaggtct gcttgcggtc ccagggacac tcggcccgca ggcgggccat gaggcggacg
     2521 agcgcggcgg ccgcctcgcc ggcggcttcg ggacgcgggg atctcggctc gggcggcacg
     2581 gaggacctcc tcgggcgggc gtcggcggga acatccccta gaatgaagcg cccgaacgag
     2641 gccttatgtc agaagcaccc aagacgatca aggtcgtgga ccgcaggatg ttcaccgccg
     2701 agggggacct gcgcgacgac gtgaaggagg agctggcggc gacgccgccc gctcccgagc
     2761 cgcccccccc cgcacccgag acggccgctc cccccgcccc cgagcagacg actccgacga
     2821 gccccgcgtt cctccggctc ctcgacatgc tcgcgcagac ggcgtcgctc tacctccagg
     2881 gcgtccccga cccggccacg gggcggcggg cggtcgacct gaacgcctcg cgcgagatca
     2941 tcgacacgct cctggccctc cgggaaaaga cccggggccg gctctccttc gaggagacgg
     3001 acgcgctcga agggctcctc ggcgagctcc agctcgtcta cagccgcctc gcgacccagg
     3061 cccgaggagg cggcgccatg ccgcctccgc cgaggccgaa aggctgaccc tgcgggagcc
     3121 gatgcgccgc cggacggggc tcggcccttc acgaccgggg gcgctgctcg cggccctcgc
     3181 gctgctcttc cccctgctcc tctcgggcct tcccgcgagc gcccaggtgc tccaggcgcc
     3241 gggcgaggcc acggagtcga ccttccgtcg cgaggtgcgc gggcagtggt tcgcgtggct
     3301 cgccgcgcac gaggaagggg accctctcct ggcggcgcag aaggtcgagg agatcgtcaa
     3361 gcacgcccga aagatcgggc tgaagcggct cacggacctt tcgctcgccg cgacgctcct
     3421 cgcgcgccgc gaggtgacgc aggggagccc cgagaaggcc cgatgggcgc tcgacgccgc
     3481 cgtccggctc gatcccgacc tgccggaggc ccgctgggcc cgcgtcgggc tcgcgctgaa
     3541 ggccgacctg ctggccgcgc cgagagagct gctcggggcg atccgcgcgt cggtggtcga
     3601 cctcgagggg aggagggtcc tctacgtccg gacctctctc ctcctcgtcc tgaccctcgc
     3661 cgcggtcggg ctctcgttcg tcgtgctcct cgtggcgggc acgtcgcgcc gactcttcca
     3721 cgacctgacc gagctggccg gacgatgggt tccgaggtcc gccgagccgg tcgtggcgct
     3781 cgccctcttc ctgctccccc tgctcctcgc cctcgacgtc ggctggctcc tcctctggct
     3841 cttcgtcctc tccctcggct acgccgagcg gaacgtgagg tgggcggccg tggcgggtct
     3901 cgtgccgctt ctgttcgtcg ccccggcgct cgaccgcgcg gccgtctccc tctcctcgtc
     3961 cgcctcgccg gtcctccggg gggccgaggc gctggaggag aagcgatacg accagcgggt
     4021 cctggacgac ctcgaggccg tgaaggcgct cttccccgag gacgccgaca tccgattcct
     4081 cctcggctgc ctctaccagc agctcggcca gaacgaccgg gcggtggccg aatacaccgt
     4141 cgcgtcccag gcctcgacgg ccgaggtccg cgcgctgatc aaccggggcg acatccgttt
     4201 cgtcgacggc gacttcggcg cggcgcagga ggacttccag gaggccctcc ggcgcgaccc
     4261 gcgcgacgtg cgcgcccgct acaacctctc cctcgtctac ggcgagacgt tccggaccgt
     4321 ggaggcgcag gagaagctga gcgaggcccg ggccctcgac aacgcgctcg tcacgcgttt
     4381 cctcgacagc ccgaccctcg tcaaggtcgt ctccctcggc ttcacgccgg acgaggcgcg
     4441 ggcgaagatc gcgctcctgc agcgcgaccc gaggagccgg cgcatcctgg gccacttcca
     4501 cctcgggagc gtcgtcgcga cgtgggccgt ccctctcgcc gtcgcgatcc cgttcgcgct
     4561 cgtcgcggcc ttcctgctcg actcgttccg ccggaagggg tacggctacg ccctcgcctg
     4621 ccagaagtgc ggccggacgt tctgccggct ctgcaagccg cccggggaga gcccgctcct
     4681 ctgctcgcag tgcgtccacg tctacctgaa gaaggacggg gtctccatcg agacgaagct
     4741 ccagaaggtc gaagaggtga agcggaggca gggtttcgaa gggcgcctcc ggcgcgtcct
     4801 gaacctcctc ctgcccggcg gggagccgtt cctggacggg agggtcgcga aggccgccgc
     4861 gatcctcgcc gtcttcttct tcgcgcttct cgcagccctc ttccgcgagc acctcgccct
     4921 ctcgccgcgc ccgggcgccg aacggctcct cgcggggacg ctcctctggg gcctcgtcgc
     4981 ggccggagca tgggtctcgg gccagctcgc ggcgcggaag gggtgacgac gtggcgctcg
     5041 aagggaccct gcacgacttc gccctggccg acatcctcca gctgatcgcg ctccagaaga
     5101 agacgggtct cctgaccctg cggggtccgg ccgacacggt catcctcggg ttcgacgcgg
     5161 ggctgctcgt ttctgcggag tcgcaggcgc ggcggatcga cacgcggctc gggaccctcc
     5221 tcgtcaagac gcgcagcctc tcgcccgagt ctctcgcgag ggcgctcgag atccaggggc
     5281 agaccttgca gcgcctcggc ttcatcctcc tgaggaacgg cttctgcgac gccgaccagc
     5341 tccgggccgg cctcgacgcg caggtccgga agatcgccta cggtctcttc cgctggaccg
     5401 acggggacta cgtcttcgac ccccaggaga agatcgacta cgaccgggag tacgtccacc
     5461 cgatccaggt cgagagcctc ctgatgga
//
LOCUS       DAOTEZ010000294         5487 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_10691_length_5487_cov_5.234536, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000294 DAOTEZ010000000
VERSION     DAOTEZ010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10691_length_5487_cov_5.234536"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="P5164_14190"
     CDS             complement(<1..821)
                     /locus_tag="P5164_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRY45090.1"
                     /translation="MAILGRYLRPHRGLVALTLLLAGLAQVLTLVDPLIFGLIVDRYV
                     LAPGGRPESELVRGALFWLLVAAGVALAARLASALQEYVMRLLVQSIGRAMFDDGLRR
                     TLRLAFQEMEERPSGETMALLTKVRTDTERFVNASVTVLYSSLVGVGFLVWYGVTRSW
                     LLIPAFGIGVLVLGSLTGLLSRRIKTVQRQVVRETTRMSGAITESLRNIELVKSLGLT
                     RAEFRRLSGYTQRIFDLEMAKVKRVRGLAFLQAAIINVLRQSILFILLWLIFRKAL"
     gene            complement(906..3062)
                     /locus_tag="P5164_14195"
     CDS             complement(906..3062)
                     /locus_tag="P5164_14195"
                     /inference="COORDINATES: protein motif:HMM:NF019314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRY45091.1"
                     /translation="MPDGAFLALVHNASLLLAMALVYDLATTRWWGDESRLRQIAVGV
                     AIGGLGIALMLSSWVLEPGIIFDTRSILLGISGLFFGTLPTVIAMAMTAALRLWQGGA
                     ATPMGVSVIVASGCIGLAWRYRRRAALADLGAKELYALGVAVHFTMLALTMTLPRGVG
                     LRVLAGIGLPVLVIYPAATAALGLLMVNRLRRKRDADQLRESEERTRAALYGIGDAVL
                     ATDERARITRLNRVAESLTGWREPDAVGRPVREVLRIRIEGPGSEEVDPVADAMRTGE
                     LRSIGRGTVLESREGVTRPVLGSVAPTHAADGAVSGAVVVVRDQTAERAREAAARERE
                     SVFRALFEQRAVGVAHVETPTGRFLRVNDRYAEILGYGRDELVDRTFRDVTHPEDVRA
                     SSELVEDLLEGRRREYTIDKRYVRKDGTLVWVTLSVSPMWAPGETPSTHIAMVLDVTE
                     RKGAEERMADLNAELERRVARRTELLTEANRELEAFSYSVSHDLRAPLRAIDGFSRIL
                     SEEKGSQLDDEGRRLLGIVSGNARKMGRLIDDLLAFSRSSRAEMRRTEVDMTSLARAA
                     FAEARLAAPSGERVELRLANLPPAQGDPDLLRQVWVNLVGNAVKFSALVEAPVVEIEG
                     AVEGRRIVYRVRDNGAGFDMAYAHKLFGVFQRLHAPSEFAGTGVGLALARRIVARHGG
                     DVSAEGAVGRGATFSFWLPLAGPPSGEGRVGPLRPE"
     regulatory      3255..3406
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            3447..4412
                     /gene="moaA"
                     /locus_tag="P5164_14200"
     CDS             3447..4412
                     /gene="moaA"
                     /locus_tag="P5164_14200"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HRY45092.1"
                     /translation="MDQPRAAGAATLRVSVTDRCNLRCAYCMPEAGVPLVPRGEIPRL
                     AELAEAVRWLAARFSVDRVKVTGGEPLVRGGLPAFVSAVAAIPGIREVSMTTNGTRLA
                     GLARELAAAGLARVNVSLDTLDPERFRELTRGGRVEEALEGVDAALDAGLTPLKLNSV
                     LRRSSFRDDVPALLSLASEKGVEVRFIELMQTGTEAGWAIAERVTGAAVREWLVSEGA
                     RVAPLPDRPNAPARRAAIDWNGRSVATGWIDPVSHAFCGDCDRLRLDARGRLRRCLMD
                     DHAFPLVDLLRRGEAGVAAAAALYVGGKRPPDEMTIGSAMASIGG"
     gene            4649..>5487
                     /locus_tag="P5164_14205"
     CDS             4649..>5487
                     /locus_tag="P5164_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45093.1"
                     /translation="MTHLSRSTVVPGACTTRGQSLRRRLAVALSVVGAAIAAATPVEA
                     GDSTITAPRIVYTGVSQTVEFAGTDAISNESRVLTMDASISGSCNPTAGNGWSISLCG
                     RVQISLTDGTGGSLYMAGTTQVQSSPAVYQTASGAIVENPTDPAVWGGPVLTIYMNGT
                     LDQLNGALADLEYIPAPDYEDGTWDEQTPPGSGTPNIDILVNDGSVSSTNATAEIILR
                     VEGPNGGPTVAGPASALTAAAGAENKFPPSTSDPAVFSVVDPELCLRSPNNRCDGAYD
                     AAPA"
BASE COUNT          821 a         2039 c         1918 g          709 t
ORIGIN      
        1 agcgccttgc ggaagatgag ccagaggagg atgaagagga tcgactgccg gaggacgttg
       61 atgatcgccg cctgcaggaa cgcgaggccc cgcacgcgct tcaccttcgc catctcgagg
      121 tcgaagatcc gctgcgtgta gcccgagagg cgccggaact cggcccgggt gaggccgagg
      181 ctcttgacga gctcgatgtt ccggagcgac tcggtgatcg cccccgacat ccgcgtcgtc
      241 tcgcggacga cctgccgctg gacggtcttg atccggcggc tcaggagacc ggtcagcgag
      301 ccgaggacga ggacgccgat cccgaacgcc ggtatcagga gccaggagcg ggtgacgccg
      361 taccagacga ggaagccgac cccgacgagc gaggagtaga ggacggtcac cgaggcgttc
      421 acgaaccgct cggtgtcggt ccgcaccttc gtcaggagcg ccatcgtctc gccgctcggc
      481 cgctcctcca tctcctggaa cgcgagccga agggtccgac ggaggccgtc gtcgaacatc
      541 gcccgcccga tgctctggac gaggagacgc atcacgtatt cctggagggc cgaggcgaga
      601 cgagcggcga gggcgacccc cgccgcgacg aggagccaga agagcgcgcc gcggacgagc
      661 tcggactcgg gacggccgcc gggcgcgagg acgtaacggt cgacgatgag gccgaagatg
      721 agggggtcga cgagcgtcag gacctgggcg agcccggcca ggaggagcgt gagcgccacg
      781 agcccgcggt gcggccgcag gtagcggccg aggatcgcca tcggggtctc gcggccggac
      841 gggttcgcgc tcgggcgggc catcgcgagc aaggtaccac tccgcccgcg gcgagcatcc
      901 ggcgcctact ccggccgaag cggcccgacg cggccctcgc ccgacggggg gccggcgagc
      961 gggagccaga acgagaacgt cgctccccgc cccaccgccc cctcggccga gacgtcgccg
     1021 ccgtgacggg cgacgatccg cctggcgagg gccagcccga cgcccgtccc ggcgaactcg
     1081 gacggggcgt ggaggcgctg aaagaccccg aagagcttgt gggcgtaggc catgtcgaag
     1141 ccggccccgt tgtcgcggac gcgatagacg atccgccgcc cctcgaccgc cccctcgatc
     1201 tcgacgacgg gcgcctcgac gagggccgag aacttcaccg cgttcccgac gaggttcacc
     1261 cagacctgcc gcaggaggtc cgggtcgccc tgggcgggcg gcaggttcgc gagcctcagc
     1321 tcgacgcgct cgcccgaagg ggccgccagc cgggcctcgg cgaacgccgc gcgcgcgagg
     1381 ctggtcatgt cgacctcggt tcgccgcatc tcggcgcgcg aggagcgcga gaaggccaga
     1441 agatcgtcga tgagccggcc catcttccgg gcgttcccgc tcacgatccc gaggaggcgg
     1501 cgcccctcgt cgtcgagctg gctccccttc tcctccgaga ggatccggga gaagccgtcg
     1561 atggcccgga gcggggcgcg caggtcgtgc gagaccgagt aggaaaaggc ctccagctcc
     1621 cggttcgcct ccgtcaggag ctcggtccgc cgggccacgc ggcgctccag ctcggcgttc
     1681 agatcggcca tccgctcttc ggcccccttg cgctccgtga cgtcgaggac catcgcgata
     1741 tgcgtcgagg gcgtctcgcc gggcgcccac atcggggaga cggaaagagt cacccagacg
     1801 agcgtgccgt cctttctcac gtagcgtttg tcgatcgtgt actcgcgccg ccgaccttcc
     1861 agcaggtctt cgaccagctc ggagctggcc cggacgtcct cggggtgcgt cacgtcgcga
     1921 aacgtccggt cgaccagctc gtcgcggccg tagccgagga tctcggcgta ccggtcgttg
     1981 accctcagga aacgtcccgt cggcgtctcc acgtgcgcga cgccgacggc gcgctgctcg
     2041 aagagcgccc ggaagacgct ctcgcgctcg cgcgccgccg cctcccgcgc ccgctcggcg
     2101 gtctggtcgc ggacgacgac gacggccccc gagacggcgc cgtccgcggc gtgggtcggc
     2161 gcgacactcc cgagcaccgg ccgcgtcacg ccctcccggg actcgaggac cgttcccctc
     2221 ccgatcgacc ggagctcacc cgtgcgcatc gcgtcggcca ccggatcgac ctcctcgctt
     2281 cccgggccct cgatccgaat gcgaagcacc tcgcgcaccg gccggccgac cgcgtccggc
     2341 tcgcgccagc cggtcagcga ctcggccacc cggttgagcc gcgtgatgcg cgcccgctcg
     2401 tcggtcgcga ggacggcatc gccgatcccg tagagggccg cccgcgtccg ctcctcgctc
     2461 tcccggagct ggtcggcgtc ccgcttcctc cgaagccggt tgaccatgag gaggcccagc
     2521 gccgccgtcg cggccgggta gatgacgagg acggggaggc cgattccggc caggacacgg
     2581 agccctaccc ccctggggag cgtcatcgtc agggcaagca tcgtgaagtg gaccgcgacg
     2641 ccgagcgcgt agagctcctt cgccccgagg tcggcgaggg ccgcgcggcg ccggtaacgc
     2701 cacgcgaggc cgatgcagcc ggaggccacg atgacgctga cgcccatcgg ggtcgcggcg
     2761 cctccctgcc agagccggag ggccgccgtc atcgccatcg cgatgacggt cgggagggtc
     2821 ccgaagaaaa ggccggaaat gccgaggagg atcgagcgcg tgtcgaagat gatccccggc
     2881 tcgagcaccc aggaggagag catgagggcg atgccgagcc cgccgatcgc caccccgacc
     2941 gcgatctgtc ggaggcggct ctcgtctccc caccagcgcg tcgtcgcgag gtcgtagacg
     3001 agcgccatcg cgaggaggag gctcgcgttg tggacgagcg cgaggaaggc cccgtcaggc
     3061 atgcgcggcc ccgtcgaacg aagccggacg ccggggcgcc gactgcccgc acggccctct
     3121 ctctcccgat tcgggtgcgg accgatcgct caccgctccc ccttctccgg ttcgcttcgt
     3181 tcgccgcggg cgcaccgtac agtctagacc tcgttccgac cgtccggcgc gacgacgcgc
     3241 gaaagcccgc gatagaattg cttcgtcttc gatccccggc ccctgaaggg aggccctccc
     3301 ggggcccggg acggtgggca ggacccggcg acgggttcga cgctccgagg gtcgcctccg
     3361 gaaacgggac ggcgtcccgc ctggaaagga gatcctcgat gccctcgccg acaccccgcg
     3421 cgacccgctc cggctgaggg gctctcgtgg accagcctcg cgccgcgggc gccgcgacgc
     3481 ttcgcgtctc cgtcaccgac cgctgcaacc tccgctgcgc ctactgcatg ccggaggccg
     3541 gggtgccgct cgtgccgcgg ggcgagatcc cgcgcctggc cgagctggcg gaggcggtgc
     3601 gatggctcgc ggcgcgcttc tccgtcgacc gcgtgaaggt gacgggaggc gagcccctcg
     3661 tccgcggtgg cctccccgcg ttcgtctcgg ccgtcgccgc gatccccgga atccgcgagg
     3721 tctcgatgac gacgaacggg acccgcctcg ccggcctggc ccgggagctc gccgcggcgg
     3781 gcctggcacg cgtgaacgtc tcgctcgaca cgctcgaccc ggaacgcttc cgggagctga
     3841 cgcgcggggg ccgtgtcgag gaggcgctcg aaggggtcga cgccgccctc gacgccggac
     3901 tgaccccgct caagctcaac tcggtcctcc gccgcagctc cttccgcgac gacgtccccg
     3961 ccctgctctc cctggcgtcg gagaagggag tcgaggtccg cttcatcgag ctgatgcaga
     4021 ccgggaccga ggccggctgg gcgatcgccg agcgcgtcac cggagccgcc gtccgggaat
     4081 ggctcgtctc cgaaggcgct cgcgtggcgc ccctgccgga ccgcccgaac gccccggcgc
     4141 gccgggcggc gatcgactgg aacggccggt ccgttgcgac cggctggatc gacccggtca
     4201 gccacgcgtt ctgcggcgac tgtgaccgtc tgcgcctcga cgcccgcggc cgccttcgcc
     4261 gctgcctgat ggacgaccac gcctttccgc tcgtcgacct gctccgccgg ggcgaggccg
     4321 gtgtcgcggc ggccgccgcg ctctacgtcg ggggcaagcg cccgccggac gagatgacga
     4381 tcggctccgc gatggcctcg atcggcggct agccggaccg cgcctcagcc cgcggccgtg
     4441 cgccccgacg aagatctgcg aggagccggc gcccgaccga gaccagccgg cgacgccggc
     4501 acgcgtcgag gacgtgacac ttcgcacaat ttgcgtcgtt gacactgacc cctcctcaaa
     4561 tagactcggt ctcaaaacca ctaagtccga atcgacctgc cgggacgacc gggagatcga
     4621 gactccttgt gcgggaggca cgcaactcat gacgcacctg agccgatcaa ccgtcgttcc
     4681 gggagcctgc acgaccaggg gccagagcct gcgccgtcgc ctggcagtcg ccctctccgt
     4741 cgtcggcgcc gcgatcgctg cggcgactcc cgtcgaggcg ggcgactcca ccatcaccgc
     4801 gccgcgaatc gtctacacgg gcgtcagcca gacggtcgag ttcgcgggca ccgacgcgat
     4861 ctcgaacgag agccgtgtgc tcacgatgga cgcgtcgatc tccggctcct gcaatccgac
     4921 ggcgggaaac ggctggtcga tctcgctctg cggccgggtg cagatcagcc tgacggacgg
     4981 caccggcggc tccctctaca tggcgggcac gacccaggtc cagtcctctc ccgccgtcta
     5041 ccagaccgcc tcgggcgcga tcgtcgagaa cccgaccgac cccgccgtct ggggtggccc
     5101 ggtcctgacc atctacatga acggcacgct cgaccagctg aacggagcgc tcgccgacct
     5161 cgagtacatc ccggcgccgg actacgagga cgggacctgg gacgagcaga cgccgccggg
     5221 ctccggcacg ccgaacatcg acatcctcgt caacgacggc tccgtcagct cgacgaacgc
     5281 cacggcggag atcatcctgc gcgtcgaggg gcccaacgga ggcccgacgg tggccggacc
     5341 ggcttcggcg ctcacggcgg ccgccggcgc ggagaacaag ttcccgccga gcacgtcgga
     5401 cccggccgtc ttctcggtcg tcgatcccga gctctgcctc agaagcccga acaaccggtg
     5461 cgacggcgcc tacgacgccg cccccgc
//
LOCUS       DAOTEZ010000295         5478 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_10717_length_5478_cov_5.865941, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000295 DAOTEZ010000000
VERSION     DAOTEZ010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5478
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10717_length_5478_cov_5.865941"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /locus_tag="P5164_14210"
     CDS             <1..1418
                     /locus_tag="P5164_14210"
                     /inference="COORDINATES: protein motif:HMM:NF012882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HRY45094.1"
                     /translation="RPHQVIDEVFDLMVDLGATDHQLDFPIVYTSAKQGFARREVDHA
                     DNDVRPLLDAILVEVPEPGGNPEAPLQILVASLDYDNYLGRLAIGRVVNGVVTPGQNV
                     SVCRHDGSIAKGRVTKLMVFEGLKRTEVPEAAAGEIVVLSGLEEVTIGETVADADFPV
                     ALDPIAVDEPTVSMTFRVNDSPFAGLEGKYVTSRNIWDRLQKELRTNVALRVEPTDGP
                     DAFKVSGRGELHLAILIETMRREGFELGLSRPEVITKEVDGVTCEPAEQLVVDVPEEF
                     VGTVIEKIGRRRGEMTALVNNGTGRARIEFKVPTRGLFGYRNEFLTDTKGEGILHHVF
                     AGYEPWKGDVETRRTGALICKEAGDTTAYAIERLEERSVFFFGPGVACYPGMIVGENA
                     RENDMIVNPCVKKHLTNMRASNSDMAIKLTPPREMPLEAAIEWIEEDELVEVTPRSIR
                     LRKRVLDHSQRKTRKPTTEAA"
     gene            1525..2334
                     /gene="zupT"
                     /locus_tag="P5164_14215"
     CDS             1525..2334
                     /gene="zupT"
                     /locus_tag="P5164_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007930991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HRY45095.1"
                     /translation="MGIAFGLTLAAGLSTGLGSAIAFFARRTNLRLLSVATGFSAGVM
                     LWVSFLEILPKSQAALATAVGARQGAWLSVGAFFLGILLILAIDRLVPGDENPHEVRT
                     ALELASLGDAMALPAAAKLRRTGTAVAVAIAIHNFPEGMATFLSALDDPKVGLAIAIA
                     VALHNIPEGVSVAVPIYYATGRRRAAFGWSFLSGLAEPVGAAVGYLVLRPFLTPGVMG
                     VVFGAVAGIMVFISLDELLPTSRAFGEGHDSLYGLLLGMGAMSLSLLLLMP"
     gene            2345..2998
                     /locus_tag="P5164_14220"
     CDS             2345..2998
                     /locus_tag="P5164_14220"
                     /inference="COORDINATES: protein motif:HMM:NF017966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromophore lyase CpcT/CpeT"
                     /protein_id="HRY45096.1"
                     /translation="MRRARSILPALLLASLPAAGAEPPSRVEEVAALLSGTFDSSAQA
                     ASDPEGFRAVRLVAVRVPASRLGAGAPVLYVEQALLESPDRPYRQRFYRIEETADGGV
                     VSRVFEPKEPVAVSGKWRDPSDLALYGAGDVVERLGCAVRLKRTEGGWGGATEGKGCP
                     SALAGARWATSEVTLRPGRLESWDRGYDVEGRQVWGARSGPYVFERRSDGPPAASPR"
     gene            complement(3003..4391)
                     /locus_tag="P5164_14225"
     CDS             complement(3003..4391)
                     /locus_tag="P5164_14225"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HRY45097.1"
                     /translation="MAEPRIIPRAEHPISRGYIDPDALKVLYRLHRAGYKAYLVGGSV
                     RDLLTGRTPKDFDVGTDARPQDVRRLFRNSRVIGRRFRLAHVLFDGGKLVEVSTFRRR
                     PDPADAEPGPGGDDDLLIRSDNTFGTPAEDAVRRDFTINGLFYDIATFAVIDWVGGVE
                     DLERHVIRTIGDPEIRFREDPVRMMRACEFAARLDFRMTPGVRAAIEENRKEIRRSAA
                     PRVTEELLDPLRRGWGAPTYRLWSETGLLGEIFPALEAEIGGAARGTGLFWKLLTETD
                     RRRGGGEVLSDSILLGVVFLPLLFAAIRARSGGSVDTQKLLLLLEEVVNPEAARLSLP
                     NAAIHDLKQGLFTMGRIAELRPGDPAARRLVTKGYFPVAVGLLSLYSAASGRYRDAAR
                     IWEDVLRRGGRTPRPGEDLRVAIPFPEALIPHEETGPAELLLPDEAPARPRPAPRRRR
                     RRGRGGRAGEAA"
     gene            4494..>5478
                     /locus_tag="P5164_14230"
     CDS             4494..>5478
                     /locus_tag="P5164_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45098.1"
                     /translation="MTEPFPRPSASEPPLVVAVALLGAAIASRFLAIAAAPGEIDEAV
                     FAGAVHWFDMADLSPQAPGFPVWILLGRALLPFASGAFHALAVAATLLSCVALPALYL
                     WGRRLVGGWAALGATVFAAFLPVVWVNGGRAFSDSPSTAFFLAALAALAAASERAASG
                     RAGAVSLAVLAGLAAAAGAGVRPHLVLAFGPLLLVEAILLVRSRSGRPVAAALVASGL
                     AGTAAWLVWLLAQAGGVQGFLAGIDERASFRSEAFEKGRFGGLLDSFLVRDFLAWPLA
                     LGVFLLALLGLGALFRRTPRAAVSLLLVLGPAFWSLWFLHSREMSRYSVPFV"
BASE COUNT          664 a         1941 c         2056 g          817 t
ORIGIN      
        1 cgcggcccca ccaggtcatc gacgaggtct tcgacctgat ggtcgacctc ggcgcgacgg
       61 accaccagct cgacttcccg atcgtctaca cctcggccaa gcagggtttc gcgcggcgcg
      121 aggtggacca cgcggacaac gacgtccgcc cgctcctcga cgcgatcctc gtcgaggtgc
      181 ccgagccggg cgggaacccg gaggcgccgc tccagatcct cgtcgcctcc ctcgactacg
      241 acaactacct cgggcggctc gcgatcgggc gcgtcgtgaa cggcgtcgtg acgcccgggc
      301 agaacgtctc ggtctgccgc cacgacggct cgatcgcgaa gggccgcgtc acgaagctca
      361 tggtcttcga ggggctgaag cggaccgagg tgcccgaggc ggccgccggg gagatcgtcg
      421 tcctctcggg cctcgaggag gtgacgatcg gggagacggt cgccgacgcc gacttccccg
      481 tcgccctcga cccgatcgcg gtcgacgagc cgaccgtctc gatgacgttc cgggtgaacg
      541 actcgccgtt cgccggcctc gaggggaagt acgtcacgag ccggaacatc tgggaccgcc
      601 tccagaagga gctccggacg aacgtggcgc tccgcgtgga gccgacggac ggccccgacg
      661 ccttcaaggt ctcgggccgg ggcgagctcc acctcgcgat cctcatcgag acgatgcgcc
      721 gcgagggctt cgagctcggc ctctcgcgtc ccgaggtcat cacgaaggag gtcgacggcg
      781 tgacgtgcga gcccgcggag cagctcgtcg tcgacgtgcc ggaggagttc gtcgggaccg
      841 tgatcgagaa gatcggccgg cgccggggcg agatgaccgc gctcgtgaac aacggcacgg
      901 ggcgcgcgcg gatcgagttc aaggtgccga cgcgcggcct cttcggctac cggaacgagt
      961 tcctcaccga cacgaagggg gaggggatcc tccaccacgt cttcgccggc tacgagccct
     1021 ggaagggcga cgtcgagacg cgccggaccg gcgccctcat ctgcaaggag gcgggcgaca
     1081 cgacggccta cgccatcgag cgtctggagg agcgctccgt cttcttcttc ggcccgggcg
     1141 tcgcctgcta tcccgggatg atcgtgggag agaacgcccg cgagaacgac atgatcgtca
     1201 acccgtgcgt caagaagcac ctgacgaaca tgcgcgcctc caactccgac atggcgatca
     1261 agctcacccc gccgcgcgag atgcccctcg aggccgccat cgagtggatc gaggaggacg
     1321 agctcgtcga ggtcacgccg cgctccatcc gcctccgcaa gcgcgtcctc gaccactccc
     1381 agcgcaagac gcgcaagccg acgaccgaag ccgcgtagcc ggagggccca aggcccggct
     1441 tccgccccgg gcgagtcgat tccggtcgtg cgaacatgat cggtgcgcct tcggcgccgg
     1501 ggaggggcta cgacggggag caacgtgggg atcgcgttcg gcctgacgct cgcggcgggc
     1561 ctgtcgacgg ggctcgggag cgcgatcgcg ttcttcgccc ggcggacgaa ccttcgcctg
     1621 ctgtcggtcg cgacagggtt ctcggcgggc gtcatgctgt gggtgtcgtt cctcgagatc
     1681 ctcccgaagt cgcaggcggc gctcgcgacc gccgtcggcg cgcggcaggg agcgtggctc
     1741 tccgtcggcg cgttcttcct cggcatcctc ctgatcctcg cgatcgaccg gctcgtgccg
     1801 ggcgacgaga acccgcacga ggtgaggacg gcgctcgagc tcgcttcgct cggcgacgcg
     1861 atggccctcc cggcggccgc gaagctgcgg aggacgggga cggcggtcgc ggtcgccatc
     1921 gcgatccaca acttcccgga agggatggcg accttccttt ccgcgctcga cgaccctaag
     1981 gtcgggctcg cgatcgccat cgccgtggcc ctacacaaca tcccggaagg ggtgagcgtg
     2041 gccgtgccga tctactacgc gacggggcgg aggcgcgcgg cgttcggctg gtcgttcctc
     2101 tcggggctcg cggagccggt cggggccgcc gtcggctatc tcgtcctgcg cccgttcctg
     2161 acgcccggcg tcatgggcgt cgtcttcggc gcggtggcgg ggatcatggt cttcatctcg
     2221 ctcgacgagc tcctgccgac gtcgcgcgcc ttcggcgagg ggcacgactc gctctacggc
     2281 ctcctcctcg ggatgggggc gatgtcgttg agcctcctcc tcctgatgcc gtagagtcgg
     2341 cgcgatgcga cgcgctcgat ccatcctccc cgcgcttctc ctcgcgagcc tcccggcggc
     2401 cggggcggag cccccctccc gggtcgagga ggtcgccgcg ctcctttcgg ggacgttcga
     2461 ctcctccgcc caggccgcct cggacccgga ggggttccgg gccgtccggc tcgtggcggt
     2521 gcgggtcccg gcgagccgcc tcggcgcggg cgcgccggtc ctctacgtcg agcaggcgct
     2581 cctcgagagt ccggaccggc cgtaccggca gcggttctac cggatcgagg agaccgcgga
     2641 cgggggcgtc gtctcgcgcg tcttcgagcc gaaggagccg gtcgcggtgt cggggaagtg
     2701 gcgcgacccg tcggacctcg ccctctacgg ggcgggcgac gtcgtggagc ggctcggctg
     2761 cgcggtccgc ctgaagcgga cggagggcgg ctggggcgga gcgaccgagg ggaagggatg
     2821 tccctcggcg ctcgccgggg cgcgctgggc gacctccgag gtgacgcttc gcccgggccg
     2881 gctggagtcg tgggaccgcg ggtacgacgt cgaaggtcgg caggtctggg gggcccggag
     2941 cggaccgtac gtcttcgagc ggcggtcgga cggtccgccc gccgcttcgc cgaggtaagg
     3001 gctcaggcgg cttcgcccgc tcggccgccg cggccgcggc gacgccgccg gcggggcgcg
     3061 ggccgggggc gcgcgggcgc ctcgtcgggg agcaggagct ccgcggggcc cgtctcctcg
     3121 tgcgggatca gcgcctcggg gaaggggatc gcgacgcgga ggtcttcgcc cggccggggc
     3181 gtccggcctc cccggcgcag gacgtcttcc cagatccgcg cggcgtcgcg gtagcggccg
     3241 gaggcggccg agtagagcga caggaggccg acggcgacgg ggaagtaccc cttcgtgacg
     3301 aggcggcgcg cggccgggtc gccggggcgc agctcggcga tccggcccat cgtgaagagc
     3361 ccctgcttga ggtcgtggat cgccgcgttc gggagcgaga ggcgagccgc ctccgggttg
     3421 acgacctcct cgaggaggag gaggagcttc tgcgtgtcga cggagccccc ggagcgggcg
     3481 cggatggcgg cgaagaggag cgggaggaag acgacgccga ggaggatcga gtccgagagg
     3541 acctcgccgc cgccgcgccg ccggtcggtt tccgtgagca gcttccagaa gaggccggtc
     3601 ccgcgcgcgg cgccgccgat ctcggcctcg agcgccggga agatctcgcc gagcagcccg
     3661 gtctccgacc agaggcggta ggtcggcgcg ccccagccgc gccggagcgg gtcgagcagc
     3721 tcctccgtga cgcggggcgc ggcgctcctt cggatctcct tccggttctc ctcgatcgcg
     3781 gcgcggactc cgggggtcat ccggaagtcg aggcgcgccg cgaactcgca ggcgcgcatc
     3841 atccggacgg ggtcctcccg aaagcgaatc tcggggtctc cgatcgtccg gatgacgtgc
     3901 cgctcgagat cctcgacgcc gccgacccag tcgatcacgg cgaacgtggc gatgtcgtag
     3961 aagaggccgt tgatcgtgaa gtcgcggcgg acggcgtcct cggcgggcgt gccgaacgtg
     4021 ttgtccgacc gaatcaggag gtcgtcgtcg cctcccggtc cgggctcggc gtcggccggg
     4081 tccgggcgcc tgcggaaggt cgagacttcc acgagcttgc cgccgtcgaa caggacgtgg
     4141 gcgaggcgga agcggcgtcc gatgacgcgg ctgttccgga agaggcggcg cacgtcctgc
     4201 ggacgggcgt ccgtaccgac gtcgaagtcc ttcggggtcc gcccggtcag gaggtcgcgg
     4261 acggagcccc cgacgaggta ggccttgtat cccgcccggt ggaggcggta gagaaccttc
     4321 agggcgtcgg ggtcgatgta gccgcgcgag atcgggtgct cggcgcgggg gatgatccgt
     4381 ggctcggcca tccctacagg cgggtcctcg agagcatcgc cccattctag cgcctgtggc
     4441 gggaggcgcc gcgacgcgtg cgggggcgcc gggcacctag aatgcgaacg cccgtgaccg
     4501 agccgttccc gcgcccgtcc gccagcgagc cgccgctcgt cgtggccgtc gccctcctgg
     4561 gagccgcgat cgcgtcgcgg ttcctcgcca tcgcggcggc gccgggagag atcgacgagg
     4621 cggtcttcgc cggggccgtg cactggttcg acatggcgga cctctcgccg caggcgccgg
     4681 ggttcccggt ctggatcctc ctcggccggg cgctcctgcc gttcgcgtcc ggcgcgttcc
     4741 acgccctggc cgtggccgcc acgctcctct cgtgcgtcgc tcttccggcg ctctacctct
     4801 gggggcggcg gctcgtcggg gggtgggcgg cgctgggagc gacggtcttc gccgcattcc
     4861 tcccggtcgt ctgggtgaac ggcggccggg ccttctccga ctcgccgtcg acggcgttct
     4921 tcctcgcggc gctcgcggcg ctcgcagcgg cctcggagcg tgccgcctcc ggccgcgcgg
     4981 gcgccgtgtc gctcgccgtc ctcgccggcc tcgcggcggc cgccggagca ggggtccgcc
     5041 cccacctggt cctggcgttc gggccgctcc tcctcgtgga ggcgatcctc ctcgtccggt
     5101 cgcggtccgg ccgccccgtc gcggcggcgc tcgtcgcgtc cgggctcgcg gggacggcgg
     5161 cgtggctcgt ctggctgctc gcgcaggccg gaggtgtgca gggcttcctc gccgggatcg
     5221 acgagcgggc ctcgttccgg tcggaggcgt tcgagaaggg gcgcttcggg ggcctcctcg
     5281 actcgttcct cgtgcgcgac ttcctcgcgt ggcccctcgc gctgggcgtc ttcctgctcg
     5341 ccctcctcgg tctcggggcc ctgttccggc gaacgcctcg cgcagccgtt tcgctcctcc
     5401 tcgtcctcgg cccggcgttc tggtcgctct ggttcctcca cagccgcgag atgtcgcgct
     5461 actcggtccc gttcgtcc
//
LOCUS       DAOTEZ010000296         5474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_10733_length_5474_cov_5.873408, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000296 DAOTEZ010000000
VERSION     DAOTEZ010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10733_length_5474_cov_5.873408"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="P5164_14235"
     CDS             complement(<1..757)
                     /locus_tag="P5164_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRY45099.1"
                     /translation="MKTRASTVRPAAEKPFAPSLVPAAPDARLLRELAGEAGKLLVAH
                     PELASPRTWIDLWNLFLHRNQSLELDDFGWDESAAKVWEPLFDFLYRVWWRVTLTGVE
                     NVPNEGRALLVINHSGVLPWDGAMLKHGLAREHPARRKARMLALDMFTTLPFLQPWLR
                     QLGEVRACPENGERLLERDELVAVFPEGVKGVGKNFRDRYRVARFGRGGFVRLALKTR
                     SPIVPVSVVGAEEIYPMLLNLAFVGRPLGFPYFP"
     gene            complement(754..1941)
                     /locus_tag="P5164_14240"
     CDS             complement(754..1941)
                     /locus_tag="P5164_14240"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRY45100.1"
                     /translation="MAGPERGGPQRPRRRPGGEVVGFTPAAGAKPKVAVTGGSGYLGA
                     RFVRALAARGTWDVVVLDLAPAANAPPEVRHRFLDLNLPHSDGTVFKILQEERPDVLV
                     HLAGLRSPLRDEVYSHELKSIGTLNVFGAAGEAGVRRLIHYSTTMVYGARGDNPAFLS
                     EEHPMRADVTDRFVRDSVEAERHCRDFARRHPDAKVAVLRFAPILAAERRDYRIRYLE
                     GPIALTMLGYDPLLQWLHPDDASEALLRAVDTPEAHGVFNVAPDGVLPLSSTLLLFGT
                     VSVPLPHAVAYAAAEAAWLAGLATAPGAHAHYVRFPCVADNAKSRRVLGFSPAKTTLE
                     TVVEAARARRGRGRAIDFDAVEEVVRTATWRLEQRLRRPAPPPPEGEAERTVRSDGTR
                     RTA"
     gene            complement(1956..2243)
                     /locus_tag="P5164_14245"
     CDS             complement(1956..2243)
                     /locus_tag="P5164_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45101.1"
                     /translation="MAETLIRQLLGRGEEVVTEVTNRLLSNEAFVEMLKKGIAVKEVV
                     DGQVAIALKRMNVATRRDLARMEARIAALEAELEASKKAPARRKPVRRKAG"
     gene            2365..4215
                     /locus_tag="P5164_14250"
     CDS             2365..4215
                     /locus_tag="P5164_14250"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HRY45102.1"
                     /translation="MAPAEPLDGLSGARLLFVSGKGGVGKTAVASALAARAAASGRRV
                     LLVSTDGRGDAAALYGRADRGYAEAELAPRLRTVTAEFEALLADFVRTIAPVGFVASR
                     ILASPTFRYFTRATPGLPDLLLLGKLRDLLAAKKRRFDLVVVDAPATGHALSLLSMPR
                     TVLRVVPAGPIHRIAAELDALFADERSAALVVVAEPAELAAREAEELVEGARARAGLR
                     AGLLVVNRVGRSGRAGTLPRGELPTIRVPELDVPRGAAESPFALDEAFFGAFRRAFDG
                     SPPPRRRGPAPPPPGPGDLDLDDALRDEPLVVLAGPGGVGKTTLAAAVGIASARSGQR
                     TLVLTVDPARRLAQALGLEGRLDRPVRVDLPGAAAFSVLQIDPRATFDRLLARVAPPA
                     TLARIRSNRLYDGLVDALPGVVEYMGVEALAEHAGDPGIDRIVLDTAPAARGVDFLKS
                     PERMVELLEHDALRWFLRSDSLLSKALSGASRGAAVVLHLADRHLGFSFLADLADFFR
                     AFDGLYDGFRERSVRIRALLDEARYLVVSSADRSALRTAAELAEMLPAGPGKAGLLLN
                     RVSPRFGLPPLPPALRTLPRRNFVEDAGAAETLPFRLADQLVSAEVASPS"
     gene            complement(4216..>5474)
                     /gene="hutH"
                     /locus_tag="P5164_14255"
     CDS             complement(4216..>5474)
                     /gene="hutH"
                     /locus_tag="P5164_14255"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HRY45103.1"
                     /translation="LPTPAVRALMLLRANCLARGFSGVRRQTVELLLAMLAAGIHPRV
                     PSQGSVGASGDLAPLAHLARAVMGEGEVEHRGTVLPAADALALAGLSPAVFEAKEGLA
                     LINGTQLTTSVGGLALARLLSLVKVADLVAALTTDALKGTDAAFDPRIHAARPHPGQA
                     ASARNLRALLAGSPLRESHRHCGTVQDAYALRCAPQVHGTVRDACAHALRVVTTEMNS
                     ATDNPMVFSTGPDGEGELISGGNFHAAPVALVFDLLTAAATDLASVSERRTERLVNPT
                     LSGDLPAFLVKEGGLHSGFMMIHVTAAALVSECKANSFPASVDSIPTSAGKEDHVSMG
                     PIAARKLARNVANLENVLAIELLAAAQALDLRRPLRSSPPLEELHARLRARVAPWDAD
                     RYAAADMDAARDVLVSGIDDLLAPLE"
BASE COUNT          680 a         2047 c         1995 g          752 t
ORIGIN      
        1 ccgggaagta ggggaagccg agcggccgcc cgacgaacgc gaggttgagg agcatcgggt
       61 agatctcctc ggcgccgacg accgagaccg gcacgatcgg cgagcgcgtc ttcagcgcga
      121 ggcggacgaa gccgccgcgt ccgaagcggg cgacccggta gcggtcgcgg aagttcttcc
      181 cgacgccctt cacgccctcg gggaagacgg cgacgagctc gtcccgctcg aggagcctct
      241 cgccgttctc ggggcaggcc cggacctctc ccagctggcg gagccagggc tggaggaacg
      301 gcagcgtcgt gaacatgtcg agcgcgagca tccgggcctt ccgccgcgcg gggtgctcgc
      361 gcgcgaggcc gtgcttcagc atcgcgccgt cccacggaag gacgccggag tggttgatga
      421 ccaggagcgc gcgcccttcg ttcgggacgt tctcgacacc ggtcagcgtc acgcgccacc
      481 agacccggta gaggaaatcg aagagaggct cccagacctt cgccgccgac tcgtcccatc
      541 cgaagtcgtc gagctccagc gactggttgc ggtgcaggaa gaggttccag aggtcgatcc
      601 aggtccgcgg cgaggcgagc tcggggtggg cgacgaggag cttccccgcc tctcccgcga
      661 gctcccggag gagacgcgcg tcgggcgccg ccggaacgag gctgggcgcg aacggcttct
      721 ccgcggcggg gcgcacggtc gaggctctcg tcttcatgcc gtcctccgcg tcccgtcgga
      781 gcggaccgtc cgctccgcct ctccctcggg gggcggcggc gcgggccggc ggagccgctg
      841 ctcgaggcgc cacgtcgccg tccgcacgac ctcctcgacc gcgtcgaagt cgatcgcgcg
      901 cccgcgcccc cgccgggcgc gggcggcctc gacgacggtc tcgagcgtcg tcttcgccgg
      961 cgagaagccc aggacgcggc gcgacttcgc gttgtcggcg acgcagggga accggacgta
     1021 gtgggcgtgc gcaccgggcg ccgtggcgag cccggccagc cacgccgcct cggctgccgc
     1081 gtaggcgacg gcgtgcggca gcgggacgga gaccgtaccg aagaggagga gcgtcgagga
     1141 gagcgggagg accccgtccg gagcgacgtt gaagacgccg tgcgcctccg gcgtgtcgac
     1201 tgcgcggagg agggcctcgc tcgcgtcgtc ggggtggagc cactggagaa gcgggtcgta
     1261 gccgagcatc gtcagggcga tcggcccctc gaggtagcgg atccgatagt cccgcctctc
     1321 ggcggcgagg atcggggcga agcgcaggac ggccaccttc gcgtccgggt ggcgccgcgc
     1381 gaaatcgcga cagtgccgct cggcctcgac ggagtccctc acgaagcggt cggtcacgtc
     1441 ggcgcgcatc gggtgctcct cggagaggaa cgccgggttg tccccccggg cgccgtagac
     1501 catcgtcgtc gagtagtgga tgaggcgccg aacgccggcc tctcccgcgg cgccgaagac
     1561 gttcagcgtg ccgatcgact tcagctcgtg ggagtagacc tcgtcgcgga gcgggctccg
     1621 gaggccggcc agatggacga ggacgtcggg gcgctcctcc tgcaggatct tgaagacggt
     1681 cccgtcggag tgcggcaggt tgaggtcgag gaaacggtgc cggacctcgg gcggcgcgtt
     1741 cgccgcgggc gcgaggtcga gcacgacgac gtcccacgtc ccgcgggccg cgagcgcgcg
     1801 gacgaatcgg gctccgaggt agccgctccc tccggtgacg gcgaccttcg gcttcgctcc
     1861 ggccgcgggc gtgaagccga cgacctcgcc gcccgggcgg cggcggggcc tctgcggccc
     1921 ccctctctcc ggtccggcca aggggcgctc ccctctcagc cggccttccg gcggaccggc
     1981 ttccgccgcg ccggggcctt cttcgacgcc tcgagctccg cctcgagagc ggcgatccgc
     2041 gcctccatcc tcgcgaggtc ccggcgtgtg gcgacgttca tccgcttcaa cgcgatcgcg
     2101 acctggccgt cgacgacctc cttgaccgcg atccccttct tgagcatctc gacgaaggcc
     2161 tcgttcgaaa gcaggcggtt cgtcacctcc gtgacgacct cctcgccgcg tcccaggagc
     2221 tgccggatga gtgtctcggc catcgttcgc ccttccttcc gcgcgatccg gacgcccgtc
     2281 gggaacgggc ctccggggat tcgtccgtgc gagaagccat tatccacgct tcgcgggccg
     2341 ggaatcgagc ggagaatgca gccgatggct ccggccgaac cgctcgacgg actctccggc
     2401 gcgcgcctcc tcttcgtctc cggcaagggc ggcgtcggca agaccgcggt cgcttccgcg
     2461 ctcgccgcgc gggccgccgc ctccggtcgg cgcgtcctcc tcgtctccac cgacgggcga
     2521 ggcgacgccg ccgcgctcta cgggcgcgcc gaccgcggct acgcggaggc ggagctcgcc
     2581 ccgcgcctcc ggacggtcac cgccgagttc gaggcgctcc tcgccgactt cgtccggacg
     2641 atcgcccccg tcgggttcgt ggcgtcgcgg atcctcgcgt cgccgacctt ccgctacttc
     2701 acgcgcgcca cgcccggcct cccggacctc cttctcctgg gaaagctgcg cgacctcctg
     2761 gcggcgaaga agcgccgctt cgacctcgtc gtcgtggacg ccccggcgac gggccacgcg
     2821 ctctcgctcc tctcgatgcc ccgcacggtg ctgcgcgtcg tgcccgccgg ccccatccac
     2881 cgcatcgccg ccgagctcga cgcgcttttc gcggacgaac ggagcgcggc gctcgtcgtg
     2941 gtcgccgagc cggccgagct ggcggcgcgc gaggcggagg agctcgtcga gggagcccgc
     3001 gcccgcgccg ggctgcgggc cgggctcctc gtcgtgaacc gcgtcggccg gagcggccgc
     3061 gccgggacgt tgccgcgcgg agaactcccc accatccggg tccccgagct cgacgtgccc
     3121 cgcggcgccg cggagagccc gttcgccctc gacgaggcgt tcttcggcgc cttccggcgc
     3181 gccttcgacg gctcgccgcc tccgcgccgc cggggcccgg ctcccccgcc tcccggtccc
     3241 ggcgacctcg acctcgacga cgcgctccgg gacgagcccc tcgtcgtcct cgccggcccg
     3301 ggaggagtcg ggaagacgac gctcgcggcc gcagtcggga tcgcgtccgc gcggtcgggg
     3361 cagcggacgc tggtcctgac ggtcgacccc gctcgtcgtc tggcgcaggc gctcgggctc
     3421 gagggccgcc tcgatcggcc cgtccgggtc gacctccccg gcgccgccgc cttctccgtc
     3481 ctccagatcg acccgcgcgc gacgttcgac cgtctcctcg ctcgggtcgc cccgcccgcc
     3541 accctcgcgc gcatccgatc caatcgtctc tacgacgggc tcgtcgacgc cctcccgggc
     3601 gtcgtcgagt acatgggcgt cgaggcgctc gccgagcacg ccggggaccc cggcatcgac
     3661 cggatcgtcc tcgacaccgc gcccgccgcc cgcggtgtcg actttctgaa gtctcccgag
     3721 cggatggtcg agctcctgga gcacgacgct ctccgctggt tcctccggag cgactcgctc
     3781 ctctcgaagg cgctctccgg cgcctcccgg ggagccgccg tcgtcctcca cctggccgac
     3841 cggcacctcg gcttcagctt cctcgccgac ctggcggact tcttccgcgc gttcgacggg
     3901 ctttacgacg gcttccggga gcgaagcgtc cggatccgcg ctcttctcga cgaggctcgc
     3961 tatctcgtcg tctcctcagc cgaccgctcg gccctccgga cggcggccga gctcgcggag
     4021 atgctgccgg cgggacccgg gaaagcgggg ctcctcctca accgcgtctc cccgcggttc
     4081 ggcctccccc cgctccctcc cgcgctccgg acgctcccga ggcggaactt cgtcgaggac
     4141 gccggcgccg cggagacgct ccccttccgc ctcgccgacc agctcgtgtc cgccgaggtg
     4201 gcttccccct cctgactact cgagcggggc gaggaggtcg tcgatccccg agacgaggac
     4261 gtcccgcgct gcgtccatgt cggccgcggc gtaccggtcg gcgtcccagg gagcgacgcg
     4321 ggcccggagg cgcgcgtgga gctcctccag cggcgggctc gagcggagcg gtcgccgcag
     4381 gtcgagggcc tgggcggcgg cgaggagctc gatcgcgagg acgttctcga ggttcgcgac
     4441 gttccgcgcg agcttgcgcg ccgcgatcgg ccccatcgag acgtggtcct ccttgccggc
     4501 cgaggtcggg atcgagtcga cggaggccgg gaaggagttc gccttgcact ccgagacgag
     4561 agccgcggcc gtcacgtgga tcatcatgaa gcccgagtgg agccctccct ccttcacgag
     4621 aaaggcgggc agatcgcccg agagggtcgg gttcacgagg cgctccgtcc tccgctcgga
     4681 gaccgaggcg aggtcggtgg ccgcggcggt caggaggtcg aagacgagcg cgaccggcgc
     4741 ggcgtggaag tttccgcccg agatcagctc cccctcgccg tccggtcccg tggagaagac
     4801 catcgggttg tcggtcgcgg agttcatctc cgtcgtcacg acgcggaggg cgtgggcgca
     4861 ggcgtcgcgc accgtgccgt gcacctgcgg cgcgcagcgg agggcgtacg cgtcctggac
     4921 cgtcccgcag tgccggtggc tctcccggag aggcgagccc gcgaggaggg cgcgaaggtt
     4981 ccgggcggag gcggcctggc cggggtgggg acgcgccgcg tggatccggg ggtcgaaggc
     5041 ggcgtcggtc cccttcaggg cgtccgtcgt cagggccgcc acgaggtccg ccaccttgac
     5101 gagagagagg agccgcgcga gggcgagccc tccgaccgag gtggtcagct gcgtcccgtt
     5161 gatcagcgcg agcccttcct tcgcctcgaa gaccgccggc gagaggccgg ccagagcgag
     5221 ggcgtcggcg gccggcaaaa ccgtcccgcg atgctcgacc tccccttccc ccatgacggc
     5281 gcgggcgagg tgggccagcg gggcgaggtc gcccgaggcc ccgaccgagc cctgggacgg
     5341 cacgcgcggg tgaatcccgg ctgcgagcat cgcgaggagg agctccaccg tctgtcgcct
     5401 caccccggag aagccgcgcg cgaggcagtt ggcccggagc agcatcagcg cgcggacggc
     5461 gggcgtcggc agga
//
LOCUS       DAOTEZ010000297         5472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_10737_length_5472_cov_7.867085, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000297 DAOTEZ010000000
VERSION     DAOTEZ010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10737_length_5472_cov_7.867085"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="P5164_14260"
     CDS             complement(<1..998)
                     /locus_tag="P5164_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HRY45104.1"
                     /translation="MPESNAPSVLASEGIEVLGPIGPQYDEILTPDALRFVASLQRAF
                     GARREELLARRVEVQKRIDSGVLPDFLPETEEIRKGGWTVAPIPPTLLDRRVEITGPV
                     DRKMVINALNSGASVFMADFEDANSPTWANTVEGQRNLRDAVDGTIAYAAPETGKEYR
                     LNERTAVLMVRPRGWHLLEKHVLVDGKPCSGSLFDFGLFLFHGGRRLIARGKTPAFYL
                     PKLEHHLEARLWNDVFTKAQEDLGVPHGTIRATVLIETVLAAFQMDEILWELRDHSAG
                     LNCGRWDYIFSFIKRLGRLPGYVLPDRGLVTMEKRFLASYVTLLIRTCHRRGIHAMG"
     gene            1111..4236
                     /locus_tag="P5164_14265"
     CDS             1111..4236
                     /locus_tag="P5164_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45105.1"
                     /translation="MRPPLASPGSGRRLRRAAGAALLLGALPLTAAPPRGRVEPGFGV
                     TPGGLVWVVEPAARRLVLRETGGVVVGAFPMAADEGYVLGANDAGARVTAPTPDGLRR
                     LPSRTAGEAHVEARYVFRGPDGSIRATARTTALRGAEPAFLGDEVWLVRRAAGSFRLV
                     RVGPDGEAPAGEIREADVRRLAGRATAPRVFAGAPASVLFPGARAEVLWTAATGLVVP
                     DPLEACGEGRSRFLYRHPEGLVRGSIRTAPSADSAWGGEPLAVAELFDAGGRVLRSAP
                     LGRWAELFPLPDGGLLGLDGMEAARFDDRFVEVSRAVFPLEEGSDAEAAARVVEQLRR
                     LERLGARASGAEWAELALLPGAPAGSHVALARRDPAGALGRLARVPDGDPSAPLAARA
                     VAMLLDLVEKEERSGLRRDLRALVEDGGSAWLRRSAAFALLEGPGEAPAWALPAVAEA
                     VASGTAEDSFELPDEAWTIELAELVTAVDRARIDRIAADRPELAEALLAGSLDDAFSS
                     SFEELRYHAPARLFARTLLDCAAGPPSVAGLLALTRVAEAAIEAAPSPAEALFGRSDE
                     AKDSRARGVLAETLLEARESPDASLRAGALALGPLTGLPLDPRRFRAEVLRRPHLGAL
                     AALGLLTDRSLPPRDWAGLFAELLAEARAASADPSACSVGAWPVLQAEGDGSRDRYCN
                     LFAIVRFAALDLGGEDDPAIVSRERVVLLAELARSPAAPPELRAELKLSRALRGTASE
                     EEIAELLDERGLAFSYRRIALEKARPPAARLAAHLERELVSGRVPLAERATWLDALAR
                     LDPGAADRVAAEAWARGSVPLDGDDGEAGAFCRALDADRVRQSEPLRRALREARARPA
                     ASLEAASLLAKAGDPGSARPLADALLERCPACTPASELAELFGPLGDSGRAELARLAE
                     ETLPFGVSPLEALFELDPAGAEALALDHLDAALARGCVPEPLLATLLGSGLDPFPALL
                     SALEERGCDRARLRPGEPVGVGLARPSGTESGDAARSALAAAPTELCRRALALLLGME
                     EERLSDRPEGR"
     gene            4233..>5472
                     /locus_tag="P5164_14270"
     CDS             4233..>5472
                     /locus_tag="P5164_14270"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRY45106.1"
                     /translation="MTAEARPLRVVVVGGGFGGLSAVRGLRGAPVEVTLVDRRNFHLF
                     QPLLYQVATGGLSPANIAAPLRALFKRQKNVRVLLGEATGVDVAGRRLLLADGELPYD
                     VLVVATGARHSYFGRPEWEDLAPGLKTLEDATRIRARVLAAFEEAEREEDPARRREAL
                     TFVVVGAGPTGVELAGALAEIARHTLRDEFRRIDPADARILLVEGTDRVLGSFAPALS
                     ARAARDVARLGIELRTGSLVTGVDPRGVTLRRGDVVEEVPARTVLWAAGVEGSPLGRL
                     VARAAGAGTDRAGRVVVEPDLTVPGRPELFVIGDLALVEPTSGRPLPGVAPVAMQQGR
                     HVARTIRARLEGRPGRPFRYVDKGSLATIGRASAVAEIGGLRFGGYPAWLAWLFVHLL
                     YIVQFGNRVLVLVQWAWSYLT"
BASE COUNT          563 a         2111 c         2061 g          737 t
ORIGIN      
        1 cccatcgcgt ggatgccgcg ccggtggcag gtgcggatga ggagcgtgac gtaggaggcg
       61 aggaagcgct tctccatcgt cacgaggccg cggtcgggga ggacgtagcc ggggagccgc
      121 ccgagcctct tgatgaacga gaagatgtag tcccagcggc cgcagttgag ccccgcggag
      181 tggtcgcgca gctcccagag gatctcgtcc atctggaacg cggcgaggac cgtctcgatg
      241 aggaccgtcg cgcggatcgt cccgtgcggg acgccgaggt cctcctgcgc cttcgtgaag
      301 acgtcgttcc agaggcgagc ctcgaggtgg tgctcgagct tcgggaggta gaaggccggc
      361 gtcttccccc gcgcgatcag gcggcggccg ccgtggaaga ggaagaggcc gaagtcgaag
      421 aggctccccg agcagggctt cccgtcgacg aggacgtgct tctcgaggag gtgccagccg
      481 cgcggcctga ccatcaggac ggcggtcctc tcgttcagcc ggtactcctt gcccgtctcg
      541 ggcgcggcgt acgcgatcgt cccgtcgacg gcgtcgcgga ggttccgctg cccctcgacg
      601 gtgttcgccc aggtcggcga gttcgcgtcc tcgaagtcgg ccatgaagac gctcgcgccc
      661 gagttgaggg cgttgatcac catcttccgg tccacggggc cggtgatctc gacgcggcgg
      721 tcgaggagcg tcggagggat cggcgcgacc gtccagccgc ccttccggat ctcctccgtc
      781 tcggggagga agtcggggag gacgccggag tcgatccgct tctggacctc gacgcgccgg
      841 gcgaggagct cctcgcgccg cgcgccgaag gcgcgctgga gggaggcgac gaagcgaagc
      901 gcgtcggggg tgaggatctc gtcgtactgc gggccgatcg ggccgaggac ctcgatccct
      961 tcgctcgcga ggacggatgg cgcattgctc tcgggcacgg gggcctcctc gttcgatcgt
     1021 ctcgggacgg cttccgcagt ttacggtgcc ccgccgcatt gcggcacgcg gccggagcgc
     1081 cggcggagcg cggatatgct tccgggaccg atgcggcccc ctctcgcatc ccctggctcc
     1141 ggccggcgtc tccgccgcgc ggcgggggcc gcgctcctgc tcggcgcgct cccgctcacg
     1201 gccgccccgc cgaggggccg cgtggagccg ggcttcggcg tgacccccgg cggcctcgtc
     1261 tgggtcgtcg agcccgccgc gcggcggctg gtcctgcgcg agaccggcgg ggtggtcgtc
     1321 ggggcgttcc ccatggcggc cgacgagggc tacgtcctcg gggcgaacga cgcgggcgcg
     1381 cgggtgacgg caccgacgcc cgacggcctc cggcgcctcc cgtcgcggac ggcgggcgag
     1441 gcgcacgtgg aggcacgcta cgtcttccgc ggccccgacg gctcgatccg cgcgaccgcc
     1501 cggacgaccg cgctccgcgg ggcggagccc gccttcctcg gggacgaggt ctggctcgtc
     1561 cggcgggcgg ccgggagctt ccgcctcgtc cgggtgggcc ccgacgggga ggccccggcc
     1621 ggcgagatcc gggaggccga cgtgaggagg ctcgccggca gggcgaccgc cccgcgcgtc
     1681 ttcgcgggcg cccccgcctc cgtcctcttc ccgggagcgc gggccgaggt cctctggacc
     1741 gcggccaccg gcctcgtcgt ccccgacccg ctcgaggcgt gcggcgaggg gcggtcccgg
     1801 ttcctctacc gccaccccga gggcctcgtg cgcgggtcga tacggacggc cccctccgcg
     1861 gactccgcgt ggggcggcga gccgctcgcc gtcgcggagc tcttcgacgc cggcggtcga
     1921 gtcctccgct cggcgccgct cggccgctgg gcggagctct tccctctccc cgacggcggc
     1981 ctcctcggtc tggacgggat ggaggcggcg cggttcgacg accgattcgt cgaggtctcc
     2041 cgcgccgtat tcccgctcga ggaggggtcc gacgcggagg cggccgcacg ggtcgtcgag
     2101 cagctccggc ggctggagcg gctcggggcg cgagcctccg gcgcggagtg ggccgagctc
     2161 gcgctcctgc cgggggcgcc cgcagggagc cacgtcgcgc tcgcgcgtcg cgacccggcc
     2221 ggggcgctcg ggcggctcgc ccgggtcccc gacggggacc cctccgcccc gctcgccgcg
     2281 cgggcggtcg cgatgctcct cgacctcgtc gagaaggagg agcgttccgg cctgcgccgc
     2341 gaccttcgcg ctctcgtcga ggacgggggg tccgcctggc tccgaaggtc cgccgcgttc
     2401 gccctcctcg agggcccggg cgaagcgccc gcctgggcgc ttcccgccgt ggccgaggcg
     2461 gtggcctccg gaaccgcgga agactcgttc gagcttcccg acgaggcctg gacgatcgag
     2521 ctcgcggagc tcgtgacggc ggtggaccgg gcccggatcg accggatcgc cgcggatcgg
     2581 ccggagctcg ccgaggcgct cctcgccgga agcctcgacg acgccttctc gtcctcgttc
     2641 gaggagctcc gctaccacgc gccggcgcgc ctcttcgccc ggacgctcct cgactgcgcc
     2701 gccgggcccc cgtcggtcgc cggcttgctc gcgctcaccc gcgtcgcgga ggccgcgatc
     2761 gaggcggcgc cctcgcccgc cgaggcgctc ttcggccgga gcgacgaggc gaaggactcg
     2821 agggcccggg gcgtcctcgc ggagacgttg ctcgaggcgc gcgagtcgcc cgacgcctcg
     2881 ctccgcgccg gagccctcgc gctcgggcct ctgacgggcc ttcctctcga cccccgccgt
     2941 ttccgcgccg aggtcctccg ccgcccgcac ctcggcgcgc tggccgcgct cggcctcctc
     3001 accgaccgct cgctcccgcc ccgggactgg gccggcctct tcgccgagct cctcgcggag
     3061 gctcgggccg cctcggccga cccgtcggcc tgctccgtcg gcgcgtggcc ggtcctccag
     3121 gcggagggcg acgggagccg ggaccgctac tgcaacctct tcgcgatcgt ccgcttcgcg
     3181 gccctcgacc tcggcggcga ggacgatccg gccatcgtct cccgcgagcg cgtcgtgctg
     3241 ctggccgagc tcgcgcgctc tccggcggcc ccccccgagc ttcgcgcgga gctgaagctc
     3301 agccgggcgc tccgcgggac ggcgtccgag gaggagatcg ccgagctgct cgacgagagg
     3361 gggctcgcct tctcctaccg ccggatcgcg ctcgagaagg cacggccgcc cgccgctcga
     3421 ctcgccgccc acctggagcg ggagctcgtc tcgggccggg tccccctcgc cgagcgcgcg
     3481 acctggctcg acgccctcgc ccgcctcgac ccgggcgcgg ccgaccgcgt cgccgccgaa
     3541 gcgtgggcaa gagggagcgt cccgctcgac ggcgacgacg gcgaggcagg tgccttctgc
     3601 cgggcgctcg acgccgaccg ggtccggcag agcgagccgc tccggagagc gctccgggag
     3661 gcgcgtgccc ggcccgccgc gagcctcgag gccgcctcgc tcctcgcgaa ggcgggtgac
     3721 cccggctccg cccgcccgct cgcggacgcg ctcctcgaac gctgtcccgc ctgcacgccg
     3781 gcgtcggagc tcgccgagct cttcgggccg ctcggagatt cggggcgggc ggagctcgcc
     3841 cgcctggcgg aggagacgct cccgttcggc gtctcgccgc tcgaggcgct cttcgagctc
     3901 gacccggcag gcgccgaggc gctggccctc gaccacctcg acgccgcgct cgcgaggggc
     3961 tgcgtcccgg agccgctcct cgccacgctc ctcgggagcg ggctcgaccc gttccccgcc
     4021 ctcctctccg cgctcgaaga acgcggctgc gaccgcgcgc ggcttcgccc cggggagccg
     4081 gtgggggtcg gcctcgcgag gccttccgga acggagtcgg gcgacgcggc ccggagcgcc
     4141 ctcgccgccg cgccgacgga gctctgccgc cgcgcgctcg ccctgctcct cgggatggag
     4201 gaggagcgcc tctcggatcg cccggagggg cgatgacggc cgaagcgcgc ccactgcggg
     4261 tcgtcgtcgt cgggggcggc ttcggcggcc tgtcggccgt ccgcggcctc cgcggagccc
     4321 cggtcgaggt gacgctcgtg gaccggcgca acttccacct cttccagccg ctcctctacc
     4381 aggtggcgac cggcgggctc tcgccggcga acatcgccgc ccccctccgg gcgctcttca
     4441 agcgccagaa gaacgtccgc gtcctcctcg gcgaggcgac gggcgtcgac gtcgcgggcc
     4501 gccggctcct cctcgcggac ggggagcttc cgtacgacgt cctcgtcgtg gccaccggcg
     4561 cgcggcactc ctacttcggc cgtcccgagt gggaagacct cgcccccggc ctgaagacgc
     4621 tcgaggacgc gacgcgcatc cgcgctcgcg tcctcgccgc gttcgaggag gcggaacggg
     4681 aagaggaccc ggcccggcgg cgggaggccc tcacgttcgt cgtcgtcggg gcgggcccga
     4741 cgggggtcga gctggccggt gcgctcgccg agatcgcgcg gcacacgctc cgggacgagt
     4801 tccggaggat cgacccggcc gacgcccgga tcctcctcgt ggaagggacg gaccgcgtcc
     4861 tcgggtcgtt cgctcccgcg ctctcggccc gggccgcgcg cgacgtcgcc cgcctcggca
     4921 tcgagctccg caccggcagc ctcgtgaccg gagtcgaccc tcgcggcgtc acgctccgcc
     4981 gtggcgacgt cgtggaggag gtccccgccc ggacggtcct ctgggcggcc ggcgtcgagg
     5041 gctcgccgct cggccgcctc gtcgcccggg cggccggagc cgggacggac cgggcggggc
     5101 gcgtcgtcgt ggagcccgac ctgacggtgc cggggcgccc ggagctcttc gtgatcggcg
     5161 acctcgcgct cgtcgagccg acctccggcc gtccgctccc gggcgtcgcg ccggtcgcga
     5221 tgcagcaggg acggcacgtg gcgcgcacga tccgggcgcg gctcgaaggg cggcccggcc
     5281 gcccgttccg ctacgtggac aaggggagcc tcgcgacgat cggccgcgcc tcggccgtcg
     5341 ccgagatcgg cggccttcgc ttcggcgggt atccggcgtg gctcgcctgg ctcttcgtcc
     5401 accttctcta catcgttcag ttcggcaacc gggtcctcgt cctcgtccag tgggcctgga
     5461 gctacctgac ct
//
LOCUS       DAOTEZ010000298         5427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_10867_length_5427_cov_4.638310, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000298 DAOTEZ010000000
VERSION     DAOTEZ010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10867_length_5427_cov_4.638310"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="P5164_14275"
     CDS             complement(<1..265)
                     /locus_tag="P5164_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRY45107.1"
                     /translation="MIRELGKGAMGVVYLGRDPVIGRLVALKTIRAVSEDDVEQREFQ
                     ERFLREAQAAGILSHPNIVTVHDVGEDAATSTSFIAMEYVEGKN"
     gene            495..2585
                     /gene="fusA"
                     /locus_tag="P5164_14280"
     CDS             495..2585
                     /gene="fusA"
                     /locus_tag="P5164_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRY45108.1"
                     /translation="MKQYPATAIRNVAVAGHNGVGKTTLVSALLHEAGAVNRFGRVDD
                     GSAPTDFDPEEIERKISIGLAVASLEWKKHKVNLIDTPGYGIFSSEMICAMRAADAAL
                     IVVDAVAGVEVQTEKAWQVADDFGLPRMIVVNRMDRENADGGRVMDQLQKKFGRSCVC
                     IEAPIGREKSFRGAIDLIGMKGFLTDDAGKTVEGPIPDEFAADAATLHEALVEMVAEG
                     DDALMETFFSQGNLSEEQMLPALRKAIHDRRIVPVLFAAAGDHERGVEKILDEIVDLL
                     PGADSFPAAALGKDGQTDVLPCDPAGPIAAIVFKTISDPFTGRLTLFRVHSGTIKADG
                     SYWNTNRETAERFGPVFSPLGKTMNTVPEVVAGDIGVVAKLKETLTGDTLSTKEKPYR
                     FPAVAIPEPAISFSIEPKSKGDEDKISLALSKLTEEDPSLKFHRDPDTSELLLSGSGQ
                     LHVEIAVARMKRKSNVEVILHPPKVPYRETITKPAEAHGRHKKQTGGHGQFADCKIRV
                     KPLPRGSDFEFVDDIFGGSIPRNYIPAVEKGIQDTRRRGYLAGYPMVDFRVELYDGQY
                     HDVDSSEMAFKIAGSLAFKDAMEKAKPTILEPIMKVGVRAPQEYMGDLMGDLTSRRGR
                     PSGMDQIGDDAVVKAEVPLSEMLSYSAVLRSLTQGRGSFTMELSHYEELPKPLQEKLI
                     AERAKLKKAEVEAE"
     gene            complement(2863..3642)
                     /gene="lpxA"
                     /locus_tag="P5164_14285"
     CDS             complement(2863..3642)
                     /gene="lpxA"
                     /locus_tag="P5164_14285"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRY45109.1"
                     /translation="MPIDRTAIVHETATVHPDAVIGPYAVVGASVVVGARTRIGAHAV
                     VDGPTVLGEDNVVHPHAALGGPPQDLKYRDEPTRLVVGSRNVFREFMTAHRGTVTGHG
                     ETVIGDDNLFMAYSHVAHDCIIGSRTVFANSASLSGHVTVEDDVILGGFVVVKQFLRI
                     GRFAYVGGMTPVNKDVLPFTWTSSDRDTKAYKINGVGLSRKGYSAERVASLNKAYRIL
                     YRNRHDHAAAIAALGELAASAPDVKVLLDFITSSQAGIHGA"
     gene            complement(3643..4119)
                     /gene="fabZ"
                     /locus_tag="P5164_14290"
     CDS             complement(3643..4119)
                     /gene="fabZ"
                     /locus_tag="P5164_14290"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HRY45110.1"
                     /translation="MSEAPTDRGGLDIRAILRTLPHRYPFLLVDRILEIVPGQKVVGL
                     KNVTINEEFFQGHFPENPVMPGVLLVEAMAQVGGFMMLAEVDDPRRRQVRLTGLDAVR
                     FRRPVVPGDQVVFTVEFVARKKSIVKVRGEARVDGQLCVEAEIMSLIQDCGREEGD"
     gene            complement(4116..5147)
                     /gene="lpxD"
                     /locus_tag="P5164_14295"
     CDS             complement(4116..5147)
                     /gene="lpxD"
                     /locus_tag="P5164_14295"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRY45111.1"
                     /translation="MARFPEPTTAAAVASACGGRLVGDAGAVVEGARSLESAGPADLA
                     FAADAKAEKAAAASAVGVLLARSALGFPGRTVVEVPDPAAALASVLEAFLPRRAPSPG
                     IHPTAIVEAGARVAADVSVGPYAVVGGETEIGPGAILEAHVVVGRRCRVGAGARLHPH
                     VVLYDDVSIGPGTEIHAGAVLGADGFGYVASQTGLKKVPQVGGVEVGSGVEIGANTCI
                     DRATLEATRVGDGTKIDDLVMVGHNCEVGAHVVLCGQVGLAGSTTVGDQTVLAGQVGT
                     GGHLRIGRGVKAGGQTGIVSDVPDGASLFGTPHLPHRDAFRVHAEMKKLPETARLVRE
                     LARAGGEKR"
     gene            complement(5155..>5427)
                     /locus_tag="P5164_14300"
     CDS             complement(5155..>5427)
                     /locus_tag="P5164_14300"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRY45112.1"
                     /translation="GRFTVVNAKHDIRRIDAELTELEKKVLPVIEAVSKELGYSLVFN
                     KFQSGLLYADDAVDITAAVITKFNTAIAAAPKTDAKPAAAAPAPKK"
BASE COUNT          857 a         1932 c         1831 g          807 t
ORIGIN      
        1 ggttcttccc ctccacgtac tccatcgcga tgaacgacgt ggacgtggcg gcgtcctccc
       61 cgacgtcgtg caccgtgacg atgttcggat gggagaggat cccggcggcc tgggcttcgc
      121 ggaggaaccg ctcctggaac tccctctgct cgacgtcgtc ctcgctgacc gcccggatgg
      181 tcttgagcgc gacgagacgg ccgatgacgg ggtcgcggcc caggtagacg acgcccatcg
      241 cgcccttccc cagctcgcgg atcacctcgt agcggccgaa cctcttcggg ggccccgagg
      301 ggaacgcacc ggatacgctc ggcagagatc ctcccgtacg cgacggcggc aataggatgc
      361 cgggactttc ccggtgtggc gcagtttagg gccgcgcgaa agagggtgtc aacgcacgaa
      421 acgccgcttc ggcgaagcag cgtcgatccc tataatgcag gcctttcgag agacctcgcc
      481 aagggggacg aagcatgaaa cagtatcccg caaccgccat tcgcaacgtg gccgtcgccg
      541 gccacaacgg cgtcgggaaa accaccctcg tctcggcgct ccttcacgag gcgggagcgg
      601 tgaaccgctt cggccgcgtc gacgacggct cggcgccgac cgatttcgac ccggaggaga
      661 tcgagaggaa gatatcgatc ggcctggccg tggcgtcgct cgagtggaag aagcacaagg
      721 tcaatctgat cgacacgccg ggatacggga tcttctcgtc cgagatgatc tgcgcgatgc
      781 gcgccgccga cgcggccctc atcgtcgtcg acgccgtcgc cggggtcgag gtccagaccg
      841 agaaggcctg gcaggtcgcc gacgacttcg ggcttccgcg aatgatcgtc gtcaaccgga
      901 tggaccgcga gaacgccgac ggcggccgcg tcatggacca gctccagaag aagttcgggc
      961 gctcctgcgt ctgcatcgag gccccgatcg gccgggagaa gtcgttccgc ggcgcgatcg
     1021 acctgatcgg gatgaagggc ttcctgacgg acgacgccgg caagacggtc gaggggccga
     1081 tccccgacga gttcgccgcc gacgcggcga cgctccacga ggcgctcgtc gagatggtcg
     1141 ccgagggcga cgacgccctc atggagacgt tcttctccca ggggaacctc tcggaggagc
     1201 agatgctccc ggcgctgcgc aaggcgatcc acgaccgccg gatcgtcccg gtcctcttcg
     1261 cggccgccgg cgaccacgag cggggcgtcg agaagatcct cgacgagatc gtcgacctcc
     1321 tgcccggcgc cgactccttc ccggccgccg ccctgggcaa ggacggccag acggacgtcc
     1381 tgccgtgcga cccggccggc ccgatcgccg cgatcgtctt caagaccatc tccgacccgt
     1441 tcaccggccg gctcacgctc ttccgcgtcc actccgggac gatcaaggcc gacgggagct
     1501 actggaacac gaaccgggag acggccgagc gcttcggacc cgtcttctcc ccgctcggca
     1561 agacgatgaa cacggtgccc gaggtcgtcg cgggcgacat cggggtcgtc gcgaagctga
     1621 aagagacgct gacgggcgac accctctcga cgaaggagaa gccgtaccgc ttccccgccg
     1681 tcgccatccc ggagcccgca atctccttct cgatcgagcc gaagtcgaag ggcgacgagg
     1741 acaagatctc cctcgcgctc tcgaagctca ccgaggagga cccgtcgctg aagttccacc
     1801 gggaccccga cacgagcgag ctgctcctgt ccggctccgg ccagctccac gtcgagatcg
     1861 ccgtagcccg gatgaagcgc aagtcgaacg tggaggtcat cctccacccg ccgaaggtcc
     1921 cgtaccgcga gacgatcacg aagccggccg aggcgcacgg ccggcacaag aagcagaccg
     1981 gcggccacgg gcagttcgcc gactgcaaga tccgggtgaa gccgctcccg cgcggaagcg
     2041 atttcgagtt cgtcgacgac atcttcggcg gctcgatccc ccgcaactac atcccggccg
     2101 tcgagaaggg gatccaggac acccggcggc gcggctacct cgcgggctac ccgatggtcg
     2161 acttccgcgt cgagctctac gacggtcagt accacgacgt cgattcgtcg gagatggcgt
     2221 tcaagatcgc cggctcgctc gccttcaagg acgccatgga gaaggcaaag ccgacgatcc
     2281 tcgagccgat catgaaggtc ggcgtccggg ccccgcagga gtacatgggc gacctgatgg
     2341 gcgacctgac gagccgccgc ggccgccctt ccggcatgga ccagatcggc gacgacgccg
     2401 tcgtgaaggc cgaggtcccg ctctccgaga tgctctccta ctctgccgtc ctccgctctc
     2461 tcacgcaggg ccgcggctcg ttcacgatgg agctctccca ctacgaggag ctgccgaagc
     2521 ccctccagga gaagctcatc gccgagcgcg cgaagctgaa gaaggccgag gtcgaggccg
     2581 agtagccttt cccgggcctg tccgcagaac ccgaaggggc cgcgcgagcg gccccttctc
     2641 cttcctgctc atcgcccggg gatgacggcg gtccgggatt cccttcgtcc cgtgcgaacg
     2701 cagcgagctg cctctccgcg cggaaggggt gcgggcctcg ccggggtccc acccccggct
     2761 cccccggcgg aacgggtccc cctcagcgcg agcggagcga gctgcctctc cgtgcggagg
     2821 ggggtacggg ggggacccgg cggaacgggc cccccccgtc cactacgccc cgtggatgcc
     2881 cgcttgtgac gaggtgatga agtcgaggag gaccttcacg tcgggggcgc tcgcggccag
     2941 ctctccgagc gccgcgatcg ccgcggcgtg gtcgtgccga ttccggtaga ggatccggta
     3001 cgccttgttg agcgacgcga cgcgctcggc cgaatagccc ttccgcgaca ggccgacgcc
     3061 gttgatcttg tacgccttcg tgtcgcggtc gctcgaggtc caggtgaacg gaaggacgtc
     3121 cttgttgacc ggcgtcatgc cgccgacgta ggcgaaccgg ccgatccgaa ggaactgctt
     3181 cacgacgacg aacccgccga ggatcacgtc gtcttcgacc gtgacgtgtc ccgagagcga
     3241 agccgagttc gcgaagaccg tccggctgcc gatgatgcag tcgtgtgcga cgtgcgagta
     3301 cgccatgaag aggttgtcgt cgccgatgac ggtctcaccg tggccggtga ccgttcccct
     3361 gtgggccgtc atgaactccc ggaagacgtt ccggctcccg acgacgaggc gggtcggctc
     3421 gtcgcggtac ttgaggtcct gcggcgggcc gccgagcgcg gcgtgcggat ggacgacgtt
     3481 gtcctcgccg aggaccgtcg gcccgtcgac gacggcgtgg gcgccgatcc gggtccgggc
     3541 gccgacgacg acggaggcgc cgacgaccgc gtaggggccg atcacggcgt ccgggtggac
     3601 ggtcgcagtc tcgtggacga tcgccgtgcg gtcgatcggc attcagtctc cctcttcccg
     3661 cccgcagtcc tggatcaggc tcatgatctc cgcctcgacg cagagctggc cgtcgacccg
     3721 ggcttcccct ctcaccttca cgatcgactt cttccgggct acgaactcca cggtgaagac
     3781 gacctggtcg cccggcacga cggggcgccg gaaccggacg gcgtcgaggc ccgtcaggcg
     3841 gacctgcctg cgccgcgggt cgtcgacctc ggcgagcatc atgaagccgc cgacctgcgc
     3901 catcgcctcc acgaggagga cccccggcat gaccgggttt tccgggaagt gtccctggaa
     3961 gaactcctcg ttgatcgtca cgttcttgag gccgacgacc ttctgccccg ggacgatctc
     4021 gaggatccgg tcgacgagga ggaacggata acgatgcggg agcgtccgga ggatcgcgcg
     4081 gatgtcgaga ccgccccggt ccgtcggcgc ttcgctcatc tcttctcccc gcccgcccgg
     4141 gcgagctcgc gcacgaggcg cgcggtctcg gggagcttct tcatctcggc gtgcacgcgg
     4201 aaggcgtccc ggtgcggcag gtgcggcgtc ccgaagaggg aggcgccgtc ggggacgtcg
     4261 ctgacgatcc ccgtctggcc gcccgccttc actccgcggc cgatccggag atggcctccg
     4321 gtccccacct gccccgcgag cacggtctgg tctccgaccg tcgtcgaacc ggcgagtccg
     4381 acctgtccgc agaggaccac atgggcgccg acctcgcagt tgtgcccgac catcacgagg
     4441 tcgtcgatct tcgtcccgtc gccgacgcgc gtcgcctcga gcgtcgcccg gtcgatacac
     4501 gtgttcgcgc cgatctcgac accggaaccg acctcgacgc ccccgacctg cgggaccttc
     4561 ttcaggcccg tctgcgacgc gacgtagccg aatccgtcgg ctccgaggac cgcccccgcg
     4621 tggatctccg tgcccggtcc gatcgacacg tcgtcgtaca ggacgacgtg agggtggagc
     4681 cgggctcccg cgccgacacg gcagcgccgt cccacgacga cgtgcgcctc aaggatcgcg
     4741 ccgggtccga tctcggtctc tccgcccacc accgcgtacg gtccgaccga gacgtcggcc
     4801 gcgacgcggg cgccggcctc gacgatcgcg gtcgggtgga tccccgggga cggcgcccga
     4861 cgcgggagga acgcctcgag gacgctcgcc agcgcggccg cggggtccgg cacctccacg
     4921 accgtccggc ccgggaaccc gagcgcggac cgcgccagga ggaccccgac cgccgaggcg
     4981 gccgctgcct tctccgcctt cgcgtcggcg gcgaaagcga ggtcggcggg acccgccgac
     5041 tcgagcgacc tcgccccctc gacgacggct ccggcgtcgc cgacgagccg cccgccgcag
     5101 gcggaagcca ccgcggcggc ggtcgtcggc tcggggaagc gggccatccc cctcttactt
     5161 cttcggcgcg ggcgccgctg cggccggctt ggcgtcggtc ttcggagcgg ccgcaatcgc
     5221 cgtgttgaac ttcgtgatga cggccgcggt gatgtcgacc gcgtcatcgg cgtagaggag
     5281 cccgctctgg aacttgttga agacgagcga atacccgagc tccttggaga ccgcctcgat
     5341 gacggggagg accttcttct cgagctccgt cagctcggcg tcgatccgtc ggatatcatg
     5401 cttcgcgttc acgacggtaa atcgtcc
//
LOCUS       DAOTEZ010000299         5425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_10870_length_5425_cov_5.173371, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000299 DAOTEZ010000000
VERSION     DAOTEZ010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10870_length_5425_cov_5.173371"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="P5164_14305"
     CDS             <1..309
                     /locus_tag="P5164_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRY45113.1"
                     /translation="KWEEMLDVVDATSPYALTGAVFSQDRYALHVMVHHLRHAAGNFY
                     INDKPTGAVVGQQPFGGARMSGTNDKAGSSLNLLRWISPRTIKETFVPATEWRYPYQG
                     "
     gene            complement(257..1861)
                     /locus_tag="P5164_14310"
     CDS             complement(257..1861)
                     /locus_tag="P5164_14310"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRY45114.1"
                     /translation="MPASRAVPSVVGVAALLLITSSAPASSVDPEPPPGSRATAATTP
                     APPLEASDLVGTWSGVASHNGETSPFALRLDLRADGKVDVYVSIPAIHLDDQPLGARP
                     LAIEGDVVRLGSWELRVDRETGTLTGVVPEGLAPLYALPLRLRRGERFAAPPRPGPGG
                     TEVAPLWVTDVKSPVWAGVTFADGLVYAGTDGGVLHALEASTGRTLWTFAAGGALRSP
                     ATVDGDTVYVPADDGVLYALGARNGKERWRQRVNPSPVVRLPFDDPKSRYDRFGSGVA
                     VSGNNLYLGTHDGHVLALDRSTGRRLWSFAADDSVLAAPSLDAGHLYFGSYGGHVYAL
                     DAGAGTLLWKHDTRRPVVSTPAVARGKILVGSRTYDFLALDAKTGVPAWTRYLWFSWV
                     ESSPAVHGAVAYVGSSDAARVYAFDVDSGRTVWETDVHGWAWGRPTVTADRVFVGTSS
                     SPGYLVGHQAAALALERATGRVVWREVVLPAGTGTYGFTGQPAVGAGLVFLGGLDGKV
                     RAYNGSRADVSPGTGTATPSPGRTSP"
     gene            complement(2187..3458)
                     /locus_tag="P5164_14315"
     CDS             complement(2187..3458)
                     /locus_tag="P5164_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45115.1"
                     /translation="MISFATLCFGLVYGVLNVEIRASGDVDRVDLVLDGRQVAELKAP
                     FAAPVDLGWQPAPHELVAIGYDEKGRELGRAKQWINRPRGNAEIFLVVEPGGTERSRL
                     ARLTWSCPTSGTAGPVEVTLDGRKLPVSDPSRIEIPVRDVSRAQILRARVECGGNVTA
                     MTETVIGGDRPSTGSPQLTAVPVHLEKGTKLPSPEGLTGWLEREGVGLRVAAVEEGGF
                     DVVFVCEGGAFEELTRLFERRGAPVRDSSDDDLRFRFLVPVGRTVDDPTIDSSSFAAT
                     AWFQRRWDAIGPTLTHGLRLKPWPRTEQRIADAVAAAGLAAAGWERRRAVVLLLGPGA
                     RNGGDLTSDEAVPFLEALRVPLFVWSIGKSPSSEPWAAATAIRTAADFDVAVARLRAE
                     LRRQRILWVEGAHLPQAVTLTPRAKGVRLAR"
     gene            complement(3532..4791)
                     /locus_tag="P5164_14320"
     CDS             complement(3532..4791)
                     /locus_tag="P5164_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45116.1"
                     /translation="MQLIASEGVSRVELILDGRPVAELRAPFAAPLDLGCEPSPHELV
                     AVAYDAEGKELGRARQWVNRPRPTAEASLVLEGGHGGSGRVAQLTWRALAREAPASVT
                     ATFDGTPIPVSDPARIELPPHEPARIHFLKTVLEFGEGVSATAELIFGGSKTVEAHSE
                     LTATAVELERKASLPPVETMAGWFEVDGIPLEVAAVDAGGIDIVFVAEGSARAGLRQA
                     FRRESLNDAETAANLRNGARFNREDLRFRFLWPVGRLTAQTEMVANVYPSTPWLSRKR
                     SSIGRAAGWLLDWPSWSRDGQRLADAVAVAGLTAAEANRKRAVVLLLGPTAKDGSLLT
                     PDEAMSFLRRLGVRLEVWSIGKRSPETLRWNARTLISAYEHREFDDAVRDLLEEVDRQ
                     RIVWLEGALLPHEVETSRFARGIRRVQ"
BASE COUNT          828 a         1952 c         1905 g          740 t
ORIGIN      
        1 aagtgggagg agatgctcga cgtcgtcgac gcgacgagcc cctacgcgct caccggcgcc
       61 gtcttcagcc aggaccgcta cgccctccac gtcatggtcc accacctgcg ccacgcggcc
      121 ggcaacttct acatcaacga caagccgacc ggcgcggtcg tcggccagca gccgttcggc
      181 ggcgcgcgga tgagcggcac gaacgacaag gcgggctcga gcctgaacct cctccgctgg
      241 atcagcccga ggacgatcaa ggagacgttc gtcccggcga cggagtggcg gtacccgtac
      301 cagggctgac gtccgcccgc gacccgttgt aggcgcggac ctttccgtcc agcccgccga
      361 ggaagaccag ccctgctccg accgcgggct gcccggtgaa gccgtaggta ccggtcccgg
      421 cgggcaacac gacctcgcgc cagacgaccc ggcccgtggc cctctcgagg gcgagggccg
      481 ccgcctggtg gcccacgagg tatcccggac tggacgacgt tcccacgaac acgcggtcag
      541 cggtgacggt cggccggccc caggcccagc cgtgcacgtc ggtctcccag acggtccttc
      601 cggagtccac gtcgaaggcg tagacacggg cggcgtccga cgagccgacg tacgcgaccg
      661 cgccgtgaac ggcgggggaa gactccaccc aggagaacca gaggtagcgg gtccaggccg
      721 gaacgccggt cttcgcgtcg agcgcgagga agtcgtaggt gcggctgccc acgaggatct
      781 tccccctcgc cacggcgggg gtcgagacca cgggccggcg ggtgtcgtgc ttccagagca
      841 gcgtcccggc gccggcgtcg agcgcgtaga cgtggccccc gtagctgccg aaatagaggt
      901 gccccgcgtc gagcgacggg gcggccagga cgctgtcgtc ggcggcgaag ctccacaacc
      961 tccggccggt ggatcgatcc agggcgagga catggccgtc gtgggtgccg aggtagaggt
     1021 tgttcccgga caccgcgaca cccgatccga acctgtcgta tcgtgatttc ggatcgtcga
     1081 acgggaggcg gacgacgggg gagggattga cgcgctgccg ccagcgctcc tttccgttgc
     1141 gcgcgccgag ggcgtagagg acgccgtcgt cggcgggcac gtagaccgtg tcgccgtcca
     1201 ccgtcgcggg tgagcgcagc gcgcctccgg cggcaaacgt ccagagcgtc ctgcccgtgc
     1261 tcgcctcgag ggcgtggagg acgccgccgt ccgtgcccgc gtagacgaga ccgtcggcga
     1321 aggtgacccc cgcccagacc ggggatttca cgtccgtgac ccacagcggg gcgacctccg
     1381 ttccgcccgg gccagggcgc ggcggcgcgg cgaagcgctc tccccgccgg aggcggaggg
     1441 gcagggcgta caggggtgcg aggccttcgg gaacgacccc ggtcagcgtc cccgtctcgc
     1501 ggtcgacccg cagctcccac gagccgaggc gcacgacgtc cccttcgatc gcgagagggc
     1561 gcgcgccgag cggctggtcg tcgaggtgga tcgccgggat gctgacgtag acgtcgacct
     1621 tgccgtcggc ccgcaggtcc agccgcaacg cgaacgggct cgtctcgccg ttgtgcgacg
     1681 ccacgccgga ccaggtgccg acgaggtcgg acgcctcgag gggaggggca ggcgtggtgg
     1741 ccgcggtggc gcgggagccg ggcggcggct cgggatcgac gctcgacgcc ggcgccgagg
     1801 acgtgatcag caggagcgct gcgacgccta cgaccgatgg gaccgctctc gatgccggca
     1861 ttggcctccc ccgtcatcgg gaatacgagg aaccgcgccg gaagttgcag gccgcgtttc
     1921 gacaggggtc gagccgggtc cgtcgtttcc gaacgatccc gggcgacctc acgccagtgc
     1981 ccgggaacag cagaatagac accggggtca ggcgtgcaat cgtgtattgt ccaagccaat
     2041 acacgattgc acgcccgacc cctactcttg ttgaccccta ctcttggccg gctcctgaag
     2101 tttcgtctcc tcgcgacaga gcggggcgga gtccgagacg atcactgtat cgatcggcat
     2161 cttcgggatg tgcccagcga gggcgctcag cgcgcgagac gaacgccctt cgcgcgaggc
     2221 gtcagcgtga cggcctgcgg caggtgagct ccctcgaccc agaggatccg ctgccggcgg
     2281 agctcggcgc gaaggcgggc gacggccacg tcgaagtcgg ccgcggttcg aatcgccgtt
     2341 gccgcagccc acggctccga cgacggtgac ttaccgatcg accagacgaa gaggggaacg
     2401 cgcagggcct cgaggaatgg cactgcctcg tcggaggtca ggtcgcctcc gttccgggcg
     2461 cccggcccga gaagcaggac gacggccctc cggcgctccc atccggcggc cgcgagcccg
     2521 gccgcggcga ccgcgtccgc gattcgctgc tccgttcgcg gccagggttt gagccggaga
     2581 ccatgcgtga gagtcgggcc aatggcgtcc caccttcgct ggaaccaggc tgtcgccgca
     2641 aaggagctcg aatcgatcgt cggatcgtcg accgtccggc cgacgggtac gaggaatcga
     2701 aaccggaggt cgtcgtcgga ggagtcgcga accggggcgc cacgcctctc gaagagtcgc
     2761 gtcagctcct cgaatgcccc cccctcgcag acgaagacga cgtcgaaccc tccctcctcg
     2821 acggcggcga cccggaggcc gactccctcc ctctcgagcc agcccgtgag cccctccggc
     2881 gacgggagct tcgtcccctt ctcgaggtgg acgggaacgg ccgtcagctg tggcgatccg
     2941 gtcgacgggc gatccccgcc gatgacggtc tcggtcatgg cggtgacgtt cccgccacac
     3001 tccacgcggg cgcggagaat ctgcgcccgg ctcacgtccc tgacgggaat ctcgattcgg
     3061 gaggggtccg aaaccgggag ctttcggccg tcgagggtca cctcgacggg cccggccgtg
     3121 cccgacgtcg ggcagctcca cgtcaggcgg gcgagacgac ttcgctccgt gcccccgggc
     3181 tccacgacca ggaagatctc cgcgttcccc cgtggcctgt tgatccactg cttcgcacgg
     3241 cccagctccc tgcccttctc gtcgtatcca atggcgacga gctcgtgagg cgcgggttgc
     3301 catccgagat cgacgggtgc ggcgaacgga gccttcagct cggcgacctg cctgccgtcg
     3361 agcacgaggt cgactcgatc gacgtcgccc gacgctcgga tctcgacgtt gaggacgccg
     3421 tagacgagac cgaagcagag agtggcgaac gagatcacgt gcccgcctcc gactcgatgc
     3481 agcgcacgag acgtcggcgc cgggagcctc gacctacgtc ccgagccgcg ctcattgcac
     3541 ccgccgaatc ccgcgcgcga atcgcgaggt ctcgacctcg tgaggcaaga gggcgccttc
     3601 gagccagacg atcctctgcc ggtcgacctc ttccaggagg tcgcgcacgg cgtcgtcgaa
     3661 ctcccggtgc tcgtaggcgg agatcagagt ccgggcgttc caccgcagcg tctccggcga
     3721 gcgctttccg atcgaccaga cctcgagccg aacgcccaga cgccggagga agctcatggc
     3781 ctcgtcgggt gtcagcaggc ttccgtcctt cgccgtggga ccgaggagga ggacgaccgc
     3841 ccgcttccgg ttcgcctcgg cggccgtgag gcccgccacg gcgacggcgt ccgcgagccg
     3901 ttgcccgtcc cggctccagc tgggccagtc gaggagccag cctgccgcgc ggccgatgct
     3961 gcttctcttc cgcgagagcc acggcgtcga cgggtagacg ttcgcgacca tctcggtctg
     4021 cgcggtcagc cgcccgacgg gccagaggaa tcggaaacga aggtcctctc ggttgaaccg
     4081 cgcgccgttc cggaggtttg cggcggtctc cgcatcgttc agcgactccc gccggaacgc
     4141 ctgcctgagc cccgcccggg cgctcccctc cgctacgaag acgatgtcga tccccccggc
     4201 gtccacggcc gcgacctcga gcggaatccc gtccacctcg aaccatccgg ccatcgtttc
     4261 gacgggaggg agcgacgcct tccgctccag ctcgaccgcc gtcgccgtca gctccgagtg
     4321 cgcctcgacc gtcttcgagc cgccgaagat cagctccgcg gtcgcggaga cgccctcccc
     4381 gaactccagg accgtcttca ggaagtggat ccgggctggc tcgtggggcg gcagctcgat
     4441 gcgagcaggg tcgctcaccg ggatcggcgt cccatcgaac gtcgccgtta ccgaggccgg
     4501 cgcctcccgc gcgagagcgc gccacgtgag ctgcgcgacc cggccgcttc ccccgtgtcc
     4561 cccttcgagg acgaggctcg cctcggccgt cggacgcggg cgattgaccc actgccgcgc
     4621 cctgccgagc tccttcccct ccgcgtcgta ggcgaccgcc acgagctcgt gcggagaggg
     4681 ttcgcacccc aggtcgagcg gcgccgcgaa gggggcacgg agctcggcga caggtcggcc
     4741 gtcgaggatg agctcgaccc ggctcacacc ttcggaggcg atcagctgca cgttgacgac
     4801 tccgataacg aacccgaccc acagagacgc gaatgcgatc atcaggcccc cgcgtaccga
     4861 ataggctacg cctgcgaccg ccaagcgagg cgcgtgccag cgagagatcc aagctcttcg
     4921 tgccaggcga acgctggccg gcgcggtcgt cggtcagcag ccgttcggcg gggcccgcat
     4981 gagcggcacg aacgacaagg ccggctcgag tctgaacctc ctccgctgga tcagcccggg
     5041 gacgatcaag gagaccttcg tcccggcgac ggagtggcgc gacccgtatc tggggtaggt
     5101 cagccgggct cggcccgaca acgggggccc tacggcgggg tcaggcgcga caccgcgtgc
     5161 ctgttgcgac ggtgagcggc ttcccgccct gacccggcac gccggacccc gcacgcccgc
     5221 ggcggccgcc gagggtccgg acgcgtgcgg ccgttccgga gcggaggtcc cggggggggc
     5281 cgtcgccacg gccgcgggct cacgaacggg tctttggtcg gcggcgcagc gaggatcggc
     5341 gcgccttcgg cgagaccggc ggccgggctg cgggcgtcgc ccgccccggc tgctcctcgc
     5401 acacctcctt gacgcagccg cggaa
//
LOCUS       DAOTEZ010000300         5397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_10946_length_5397_cov_4.684201, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000300 DAOTEZ010000000
VERSION     DAOTEZ010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10946_length_5397_cov_4.684201"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="P5164_14325"
     CDS             <1..220
                     /locus_tag="P5164_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45117.1"
                     /translation="YISMAADRIIAQPATLTGLLTALLPDAARRATLGEAARRRAEER
                     YRWDDVTDAYEALLSGRDPNGLRSGPEC"
     gene            214..1653
                     /locus_tag="P5164_14330"
     CDS             214..1653
                     /locus_tag="P5164_14330"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRY45118.1"
                     /translation="MLKQQARSVAASLYAADLTSTLGALAAAYLVRSELLPRFFPERL
                     TPLYPFTWYLVLVGPVVVIWTGLLFLAGAYRSRRTSGLKDEALLVGKIVLGGTVLLTI
                     LVYGLRLDFISRPFLPLFAVLNTVFLVVERLTVRIVARRVRARGFNYRTVVIIGDTPR
                     ARSMARLIHDHPWWGLRLLGVVRERPASSEAGTTAGGLPVLGSLEDFPTILTSLPVDE
                     VILAVDRGDVDQLEDVFLMCEEMGVKTRLILDFFPHVFAKVELEEFDGTPLLTFSTTP
                     AETGALLVKRVVDVGLALLLGIVCLPLAALLALLIKLTSHGPVLFRQVRCGLSGRPFT
                     FYKLRTMTEDAEERLSEVAHLNEHEGPVFKSSHDPRVTPFGRFLRRFSLDEIPQLWNV
                     LKGEMSLVGPRPPLPDEVARYEKWQRRRLSMKPGLTGLWQVSGRSDLPSFDQWMELDL
                     AYIDNWSLTLDTKILLRTIPTVLSGKGAR"
     gene            1745..4855
                     /locus_tag="P5164_14335"
     CDS             1745..4855
                     /locus_tag="P5164_14335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRY45119.1"
                     /translation="MRPSRPLRSGPALASLALAALVVLAPGAPAEPAAATGTTPTGRT
                     TFRAYGVERGLGDFLILTLFQDRRGFLWAGTEDGLFRYDGREFERFGRTEGLPSTYVG
                     AMCEGADGTLWAGTFAGLARWADGAFEPLAGSSGMPAEPVRALQGTPDGRLWVGLRSG
                     LYFGTSTRGFFPAPGWPGGDATALALDGQGRLWAAASPAAEGGRTRVLTLTDSGWREW
                     TLPGLAGERLTALAGDGAGRVWARSSTHLFVLDPGADAFRQAQPPAGVESQLGRLAVD
                     RDGRILVPTETGLLVLHEEGWRLVGRDQGLPTGWAQVALVDREGSLWIGSTGLHRQLG
                     GGAWHGYTTVDGLPSEVVRALFRSTDGTLWVGTEKGLARAGATRFEPLAGTERNGIRT
                     IQEDRAGNLYLAGVPVEVLRVAPRGGRIERFGAESGLRGTRVLRMVLDASGTLWVAAE
                     GGGLRAAPTGPGPLRFEPVVVPGGNETEYFSGLILDGKGRLWAAGEKGLAVLDGGRWT
                     RFTDADGLRETHVAYALSLRSGELVVAYFEGAGLSRVTYDAGRISVADLLAPGSVLAT
                     QKVYMLGEDRSGRVWAGTGVGVTSVENGTERRYRAEDGLVGEDFNNMTFLEDADGSLW
                     FGTMSGLARFDPRVTPPSVPPPATAILETKLGSRSWKGPAPDGERLGYRENVLEVRYA
                     GLSFVRENAVESETRLVGLEPEWRASRIRELRISALPPGGYRFEVRSRAGAGDWGPVA
                     GFDFSIAPAWWQSLWFRALALLALAGLVALAVQRRLRKLQRRTAILESLVGERTRELK
                     AANEALRNQSLTDPLTGLRNRRYLGVSVPEVVAQVNRSHRNVALGRGSRASVNVDLVF
                     LMVDVDHFKSVNDAHGHHAGDEVLRQLAAVLRSATRETDTVVRWGGEEFLVVGRNTCR
                     KECAVLAERIRERVGAHEFRLDDGTVLRRTCSVGFASYPVVGSTPDHVEWEQVVDLAD
                     QALYAAKIGGRNAWVGLVARDDLGVDDVTRSPAQELAALVERGAFDVRTSLPEASVLS
                     WKAHKD"
     gene            complement(4901..>5397)
                     /locus_tag="P5164_14340"
     CDS             complement(4901..>5397)
                     /locus_tag="P5164_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRY45120.1"
                     /translation="ANIGAARAAFFPRISLTAGLGTSSPDLDGLFKSGTRMWTFAPQI
                     VSPLFASGSLFANLKVSKVDREIAVATYEKAIQAAFAEVSDALALRTTLVAQREAQDA
                     LVVALEETLRLSDARYKGGIDAYLPVLLAQQSLFGAQRAAVGVRFAEQANLVTLYKVL
                     GGGV"
BASE COUNT          683 a         2047 c         1890 g          777 t
ORIGIN      
        1 ctacatatcg atggcggccg accgcatcat cgcccagccc gcgacgctca ccgggctcct
       61 caccgccctc ctccccgacg cggcacgacg cgccaccctc ggcgaggcgg cccgccggcg
      121 cgccgaggag cgctaccgct gggacgacgt caccgacgcc tacgaggcgc tcctctccgg
      181 gcgggacccg aacgggctac gatcggggcc ggaatgctga agcagcaggc gaggtccgtc
      241 gccgcctcgc tctacgccgc cgacctgacg tccacgctcg gggccctcgc ggccgcgtac
      301 ctcgtccgga gcgagctcct gccgcgcttc ttcccggagc gcctgacgcc cctctacccg
      361 ttcacctggt acctcgtcct cgtcggcccg gtcgtcgtca tctggaccgg actcctcttc
      421 ctggccggcg cctaccggag ccgccgcacc tcggggctga aggacgaggc gctcctcgtc
      481 gggaagatcg tcctcggagg gacggtcctc ctgacgatcc tcgtctacgg cctgaggctc
      541 gacttcatct cccggccgtt cctcccgctc ttcgcggtcc tcaacacggt cttcctcgtc
      601 gtcgagcggc tcaccgtccg gatcgtggcg cggagggtcc gcgcccgcgg cttcaactac
      661 cggaccgtcg tgatcatcgg cgacacgccg cgcgcccggt cgatggcgcg cctcatccac
      721 gaccatccct ggtgggggct tcggctgctc ggcgtcgtcc gcgagcgccc cgcctcctcg
      781 gaggccggga cgaccgcggg agggctgccc gtcctcggct cgctcgagga cttcccgacg
      841 atcctcacct ccctccccgt cgacgaggtg atcctcgcgg tcgaccgcgg cgacgtcgac
      901 cagctcgagg acgtcttcct gatgtgcgag gagatgggcg tgaagacgcg gctcatcctc
      961 gacttcttcc cccacgtctt cgcgaaggtg gagctcgagg agttcgacgg cacgccgctc
     1021 ctcacgttct cgacgacccc cgccgagacc ggcgcgctcc tcgtcaagcg cgtcgtcgac
     1081 gtcggcctcg cgctcctcct cggcatcgtc tgccttccgc tcgccgcgct tctcgccctc
     1141 ctgatcaagc tgacctcgca cgggcccgtc ctcttccgcc aggtgcgctg cggcctgagc
     1201 gggcgcccct tcacgttcta caagctccgg acgatgaccg aggacgccga ggaacggctc
     1261 tccgaggtgg cgcacctgaa cgagcacgag ggcccggtct tcaagtcgtc gcacgacccg
     1321 cgcgtgacgc cgttcgggcg tttcctccgc cgcttctcgc tcgacgagat cccgcagctc
     1381 tggaacgtcc tgaaggggga gatgtcgctc gtcggcccgc gcccgccgct ccccgacgag
     1441 gtcgcccgct acgagaagtg gcaacgccgg cgcctctcca tgaagccggg cctgaccggc
     1501 ctctggcagg tctcggggcg gagcgacctg ccgagcttcg accagtggat ggagctcgac
     1561 ctcgcctaca tcgacaactg gtcgctcacg ctcgacacga agatcctcct cagaacgatc
     1621 ccgacggtcc tgagcggcaa gggcgcgcgc tgaggcgtcg agctcccggt cccgggacgc
     1681 agagactaaa ctgagcgggg gcgtgccccg gtccccgccc gctgcgcccg cggaggagtc
     1741 ccgcatgcgt ccgtcccgcc cgctccgttc cggccccgcc ctcgcatccc tcgccctcgc
     1801 cgcgctcgtc gtgctcgcgc ccggcgcccc tgcggagccg gcggccgcga ccgggaccac
     1861 ccccaccggg cggacgacgt tccgtgccta cggcgtggag cgcggcctcg gcgacttcct
     1921 catcctgacc ctcttccagg accggcgggg cttcctctgg gcagggaccg aggacggcct
     1981 cttccgctac gacggccgcg agttcgagcg cttcgggcga accgaggggc tcccctccac
     2041 ctacgtcggc gcaatgtgcg aaggagccga cggaacgctc tgggccggga cgttcgcggg
     2101 cctcgcccgg tgggcggacg gcgcgttcga gccgctcgcc ggctcgtcgg gcatgcccgc
     2161 cgagccggtg cgcgccctgc aggggactcc cgacggccgg ctctgggtcg ggctgcggag
     2221 cgggctctac ttcggcacga gcacgcgcgg gttctttccc gccccgggct ggcccggcgg
     2281 cgacgccacc gccctcgccc tcgacggcca ggggcgtctc tgggctgccg cgtcgccggc
     2341 ggcggaggga ggacggacgc gggtcctcac gctcacggat tcgggctggc gcgagtggac
     2401 gctgccgggc ctcgccggcg agcgtctcac cgcgctcgcg ggcgacgggg ccggccgcgt
     2461 ctgggcgcgt tcgtccacgc atctcttcgt cctcgatccc ggcgccgacg cgttccggca
     2521 ggcccagcct cccgccggcg tcgaaagcca gctcgggcgc ctcgccgtcg accgcgacgg
     2581 ccgcatcctc gtccccaccg aaacggggct cctcgtcctc cacgaggagg ggtggcgcct
     2641 cgtcggcagg gaccagggcc ttccgacggg atgggcgcag gtcgccctcg tcgatcgcga
     2701 ggggtccctc tggatcggga gcacgggcct ccacaggcag ctgggcggcg gcgcatggca
     2761 cggctacacg acggtcgacg gcctcccgag cgaagtcgtc cgcgccctct tccggagcac
     2821 cgacgggacg ctctgggtcg ggacggagaa ggggctcgcc cgggccggcg ccacccgctt
     2881 cgagcccctc gccgggaccg aacggaacgg catccggacg atccaggagg accgtgccgg
     2941 caacctctac ctcgccggcg tcccggtcga ggtgctgcgc gtcgcacctc gcggcggccg
     3001 gatcgagcgg ttcggcgccg agtcggggct tcgcgggacg cgcgtcctcc ggatggtcct
     3061 cgacgcctcc ggcacgctct gggtcgcggc cgaaggagga ggcctccgcg cggcgccgac
     3121 gggacccggg ccgcttcgct tcgagccggt cgtcgttccc ggcggcaacg agaccgagta
     3181 cttcagcggc ctcatcctgg acgggaaggg gcgactctgg gccgccggcg agaaaggcct
     3241 cgccgtcctc gacggcggga ggtggacgcg tttcaccgac gcggacgggc tccgcgagac
     3301 gcacgtcgcc tacgccctct ccctccggag cggagagctc gtcgtcgcgt atttcgaggg
     3361 cgcgggcctg agccgggtga cgtacgacgc ggggcggatc tccgtcgccg acctcctcgc
     3421 cccgggaagc gtccttgcga cccagaaggt ctacatgctc ggcgaggacc gttccggacg
     3481 ggtctgggcc ggcacgggcg tcggcgtcac ctccgtcgag aacggcacgg aacgccgcta
     3541 tcgcgccgag gacgggctcg tcggcgagga cttcaacaac atgacgttcc tggaggacgc
     3601 ggacgggagc ctctggttcg ggacgatgtc gggcctcgcc cggttcgacc ctcgcgtgac
     3661 gcccccctcc gtcccgccgc cggcgacggc gatcctcgag acgaagctcg gttcgcgctc
     3721 ctggaagggg cccgctcccg acggcgagcg cctcggctac cgcgagaacg tcctcgaggt
     3781 gcgttacgcc ggcctgagct tcgtccgcga gaacgccgtc gagagcgaga cccggctcgt
     3841 cggcctcgag ccggagtggc gcgcctcgcg catccgggag ctccgcatct cggcgctccc
     3901 cccgggcggg tatcggttcg aggtccgcag ccgcgccgga gcgggcgatt ggggccccgt
     3961 cgccggtttc gacttctcga tcgcccccgc ctggtggcag agcctctggt tccgagccct
     4021 cgcgctcctg gccctggcgg gcctcgtcgc gctcgccgtc cagcggcggc tccgaaagct
     4081 ccagcggcgg acggcgatcc tcgagtcgct cgtcggcgag aggacccgcg agctgaaggc
     4141 cgcgaacgag gctctccgga accagagcct cacggacccg ctgacgggcc tccggaaccg
     4201 gcgctacctc ggagtctccg tccccgaggt cgtggcgcag gtgaaccgga gccaccggaa
     4261 cgtcgccctg ggccggggca gccgtgcctc ggtcaacgtc gacctcgtct tcctgatggt
     4321 cgacgtcgac cacttcaagt cggtcaacga cgctcacggc caccacgcgg gcgacgaggt
     4381 cctccggcag ctcgcggcgg tcctgcgctc ggcgacgcgc gagaccgaca ccgtcgtccg
     4441 gtggggcggc gaggagttcc tcgtcgtcgg gcgcaacacc tgccggaagg agtgcgccgt
     4501 cctggccgag aggatccgcg agcgggtcgg ggcgcacgag ttccgcctcg acgacgggac
     4561 ggtcctgcgc cggacctgct cggtcggctt cgcctcgtat ccggtcgtcg gctcgacgcc
     4621 cgaccacgtc gagtgggagc aggtcgtcga cctcgccgac caggccctct acgccgcgaa
     4681 gatcggcgga cggaacgcct gggtcggcct cgtcgcccgc gacgacctcg gcgtggacga
     4741 cgtcacgcgc agccccgcgc aggagctcgc cgcactcgtc gagcgcggcg ccttcgacgt
     4801 tcggacgagc ctccccgagg cctcggtcct gagctggaag gctcacaagg actgagcccg
     4861 gcccgggccg cccgggccgg ggccggggtc gttcggctgc tcagacgcct ccgccgagga
     4921 ccttgtagag cgtcacgagg ttcgcctgct ccgcgaagcg gacgccgacg gccgcgcgct
     4981 gcgccccgaa gagcgactgc tgggcgagga ggaccggcag gtaggcgtcg atccctccct
     5041 tgtagcgcgc gtcggacagg cggagcgtct cctcgagggc gacgacgagc gcgtcctgcg
     5101 cctcccgctg ggcgacgagg gtcgtccgga gcgcgagcgc gtcgctcacc tcggcgaagg
     5161 cggcctggat cgccttctcg taagtcgcga ccgcgatctc ccggtcgacc ttcgagacct
     5221 tcaggttcgc gaagagcgag ccgctcgcga agagggggga gacgatctgc ggcgcgaagg
     5281 tccacatcct cgtcccggac ttgaagaggc cgtcgaggtc ggggctcgag gtgccgaggc
     5341 ccgccgtgag ggagatccgc gggaagaacg ccgcgcgggc ggcgccgatg ttcgcgt
//
LOCUS       DAOTEZ010000301         5394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_10956_length_5394_cov_5.216333, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000301 DAOTEZ010000000
VERSION     DAOTEZ010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10956_length_5394_cov_5.216333"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="P5164_14345"
     CDS             <1..782
                     /locus_tag="P5164_14345"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRY45121.1"
                     /translation="LQPGVSCGSCEFCRDGERSLCVGYGILGEHRSGTLAERIVVPRE
                     NVWKAPAHLTDEEAAAFPLAAMTAWRMLVTRAALRPGESVLIHGIGGGVSAYALQIAR
                     LAGASLVIATSGDERKLERAKALGADVVLDYRATDVGKKVRELTGKRGVDVVVDSVGA
                     ATWRASLTAAAKKGRIVTCGATSGPNPEEEIRIIFWKQLSILGSTMGTDAEFAALLRA
                     VEGRRIVPVVDAVFPLENVRAAYERLESGAGFGKVVVRVAP"
     gene            complement(789..1301)
                     /locus_tag="P5164_14350"
     CDS             complement(789..1301)
                     /locus_tag="P5164_14350"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRY45122.1"
                     /translation="MRELHAGRYLRLVSRNGWEFVERPGIRGIVLVVAVTPAGDLLLV
                     EQRREAVEAVVLELPAGLAGDDESPADEPLEEAARRELLEETGWAARSMERIAEGPPS
                     AGVTSEIVTLFRARGLERVGPGGGIEGEEITVREVPLAEAEAFLERRAREGVLVDPKV
                     WAGLLFARRT"
     gene            complement(1298..3328)
                     /locus_tag="P5164_14355"
     CDS             complement(1298..3328)
                     /locus_tag="P5164_14355"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY45123.1"
                     /translation="MFAVPAPMPYSPTAASGHFVVRLVGDADAVGWVTTNLRGPGFGV
                     EPMPFESAPGLSELPGPIVWVARPGEPEPGRTLVDDAAVWSFPCLVVIPPGGAPPDII
                     AGHPSVESFEVVREPEDAPVLRMRLERLLLLHRRRAQTEAILHDLPVIVYTRTLDGIV
                     TSLNAEAETFVGRSRADVVGRPLSEILPDVTFAEKSVTEMNEGLLARGRHRVRIIAET
                     AQGRRSALDARALLLRDGHGIPWGAQVVMSDLTDEEEAQERLRVEADRNEILATIATA
                     TRDVTDLGRVFEQAIEILGLRSGARSVAILLPKEGSDAFVTRCSWRVSADLPSTAGSE
                     WHPAEIGPLRSLVSDREPWIIRDGAEPGVHAPAAEALARVGARAGIVLPVFHEEEVLA
                     CLGLSFGEPREFPQDEIRFLTLVADQLALAVRAARLYQSLAEKLRALAEEQRLRERAD
                     NDRQRLLSMLVHDLKNPLAAVLASLELSLERIAGDDRLERLLRTSTASARSLAGMIDD
                     ALLVYRTGDTPASRRAEDVASVLAQPFEEARWLAAARQVDLSIDLPDGLPPVSLDAGR
                     YRRAAANILSNALKFSRRGGDVRVSVDVEEDGGSSWLVLRVADSGPGFPESVRDRIGA
                     AYQRFAGSERIPGTGLGLTVVAAVAAAHEGQMHVEPRARPAGGSVFSLRVPA"
     gene            3381..4247
                     /locus_tag="P5164_14360"
     CDS             3381..4247
                     /locus_tag="P5164_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45124.1"
                     /translation="MLRKAALLLASLVASGAFAEGPRREASYVLSEGRTVVSPAGERA
                     SAVRGTVVVRGGTARWDLESGTFPRSTASTILLGDRGGWLLERDESVAARATAEDLTA
                     LWVPAPTGEEGPFRSSVRGLAIQPPTAAPGPVFEGRPTKRHRLTADWSLETSTPGRVS
                     TARTRLVATVVAVEDVDEEVLSPLDGFDRLLDLPGPVREEIAAALGAIPGWPVSVIVE
                     TESEHSSELPGIQAASPEGRRPLKTRVEAVRKVSALVLRPAGEASSAFTLPERTRVVG
                     IERLVTPRESLR"
     gene            4244..4945
                     /gene="radC"
                     /locus_tag="P5164_14365"
     CDS             4244..4945
                     /gene="radC"
                     /locus_tag="P5164_14365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRY45125.1"
                     /translation="MTQPGAAGGLLAGLAPEDRPREKLVARGAEALTDEELVAILLGT
                     GRAGAGVLETAREVLRGGGLRGLLARSERGRVRELARGIGVAKACRIAAAAELARRLA
                     RAEVAERPLIDAPEAAGAYLAATLAAELREVMGGLLLDAKNRLIHDAVVFRGGVTSAS
                     VQPGPLLRIAVLEGAAALVLYHNHPSGDPTPSPEDRATTARFVAAGEAVGVPVRDHVV
                     VGRGCWFSFRREGLM"
     gene            5006..>5394
                     /locus_tag="P5164_14370"
     CDS             5006..>5394
                     /locus_tag="P5164_14370"
                     /inference="COORDINATES: protein motif:HMM:NF020892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRY45126.1"
                     /translation="MSRYYTTPIYYVNDRPHIGHLYTTLVTDTLTRFHRLQGEDVRFL
                     TGTDEHGQKIEKVAKKEGVAPIAVADRHAPTFRDLWKRFSVANDDFLRTTEPRHRVGV
                     TEMIRRLGAAGDLYVAGHEGWYCVGCEA"
BASE COUNT          733 a         1921 c         2030 g          710 t
ORIGIN      
        1 tcctgcagcc gggagtctcg tgcgggagct gcgagttctg ccgggacggc gagcgcagcc
       61 tctgcgtcgg ctacgggatc ctcggggagc accgctccgg cacgctggcg gagcgaatcg
      121 tcgtgccgcg cgagaacgtc tggaaggctc cggcccacct gacggacgag gaggcggcgg
      181 ccttcccgct cgccgcgatg accgcctgga ggatgctcgt gacccgggcg gcgctccggc
      241 cgggagagag cgtcctgatc cacggaatcg gcggcggggt ctcggcctac gccctccaga
      301 tcgcgaggct cgccggggcg tcgctcgtca tcgcgacctc gggcgacgag cggaagctcg
      361 agagggcgaa ggcgctcggg gccgacgtcg tcctcgacta ccgcgcgacg gacgtcggga
      421 agaaggtccg cgagctgacc ggaaagcgcg gcgtcgacgt cgtcgtcgac tcggtcggcg
      481 ccgccacctg gcgcgcgtcg ctcacggcgg ccgcgaagaa ggggcggatc gtcacctgtg
      541 gggcgaccag cggtccgaac cccgaggagg agatccggat catcttctgg aagcagctct
      601 cgattctcgg ctcgacgatg gggaccgacg cggagttcgc ggctctcctg agggccgtgg
      661 agggacggcg aatcgtcccg gtcgtcgacg cggtcttccc tctcgagaac gtccgcgcgg
      721 cctacgagcg cctcgagtcg ggcgcgggat tcggcaaggt cgtcgtgagg gtcgcgccgt
      781 aggccgtctc aggtccgtcg ggcgaacagg agccccgccc agaccttcgg gtcgacgagg
      841 actccttcgc gcgcacgcct ctccaggaac gcctcggctt cggcgagcgg gacctcgcgc
      901 acggtgatct cctcgccttc gatcccccct ccggggccga cgcgctcgag cccgcgggcg
      961 cggaagagcg tcacgatctc ggaggtgacg ccggccgagg gggggccttc ggcgatccgc
     1021 tccatcgacc gtgcggccca gcccgtctcc tcgaggagct cccggcgggc ggcctcctcg
     1081 aggggctcgt ccgcggggct ttcgtcgtcc cccgcgaggc cggccggtag ctcgaggacg
     1141 acggcctcga ccgcctcgcg ccgctgctcg acgaggagga ggtcgcccgc gggagtcacc
     1201 gcgacgacga ggacgatccc gcggatcccg ggccgctcga cgaactccca gccgtttctc
     1261 gagacgaggc gaaggtaccg gcccgcgtgg agctccctca cgcggggacc cgcagcgaga
     1321 agacggaccc gcccgcggga cgggcccggg gctcgacgtg catctgcccc tcgtgcgccg
     1381 cggcgaccgc cgcgacgacc gtgagcccga gccccgtccc ggggatccgc tccgacccgg
     1441 cgaagcgctg gtaggcggct ccgatgcggt ccctcaccga ttcgggaaag ccgggaccgg
     1501 agtcggcgac ccggaggacg agccaggacg atccgccgtc ctcctcgacg tcgaccgaga
     1561 cccggacgtc gccgccgcgg cggctgaact tcagggcgtt cgacaggatg ttggccgcgg
     1621 cccggcgata gcggccggcg tcgagagaga cgggcggcag gccgtcgggg aggtcgatcg
     1681 agaggtcgac ctgtcgcgcg gccgccagcc atcgagcttc ctcgaacggt tgcgcgagga
     1741 cggaggcgac atcctcggcc cttcggctgg cgggggtgtc tcccgtccgg tagacgagga
     1801 gcgcgtcgtc gatcatcccg gcgagcgagc gggcggaggc cgtcgaggtc cggaggaggc
     1861 gctcgaggcg gtcgtcgccg gcgatccgct cgagcgaaag ctcgagggag gcgaggacgg
     1921 cggccagcgg attcttcagg tcgtggacga gcatgctcag gagccgctgg cggtcgttgt
     1981 cggcgcgctc gcgcaggcgc tgctcctccg cgagggcacg gagcttctcg gcgaggctct
     2041 ggtagaggcg ggcggcgcgg acggcgaggg cgagctggtc ggcgacgagc gtgagaaaac
     2101 ggatctcgtc ttgcgggaac tccctcggct cgccgaagga gagcccgagg cacgcgagaa
     2161 cctcctcctc gtggaaaacg gggaggacga tccccgccct tgccccgacg cgcgcgagcg
     2221 cctctgcggc gggagcgtgg actcccggct cggcgccgtc gcggatgatc cacggttcac
     2281 gatcggagac gaggctgcgg agcggtccga tctccgcggg gtgccactcg gagccggcgg
     2341 tggacgggag gtctgccgat acccgccagg agcagcgggt cacgaaggcg tcgctcccct
     2401 ccttcgggag gaggatcgcg acgctccggg ctccggagcg gagccccagg atctcgatcg
     2461 cctgctcgaa gacccgcccg aggtccgtca cgtcgcgggt cgccgtcgcg atcgtcgcga
     2521 ggatctcgtt ccggtcggcc tcgacccgca gccgctcctg cgcctcctcc tcgtccgtca
     2581 ggtccgacat gacgacttgc gcgccccatg ggatcccgtg cccgtcgcgg aggagcagcg
     2641 cccgcgcgtc gagggccgag cgccggccct gcgccgtctc cgcgatgatc cggaccctgt
     2701 gcctcccccg ggcgaggagg ccctcgttca tctccgtcac gctcttctcg gcgaaggtca
     2761 cgtcggggag gatctcggag agtggacggc cgacgacgtc ggcccggctc cgtccgacga
     2821 aggtctccgc ctcggcattc aggcttgtca cgatgccgtc gagcgtccgc gtgtagacga
     2881 tgaccgggag gtcgtggagg atcgcctcgg tctgggcgcg ccgccggtgg aggaggagga
     2941 gccgctcgag tcgcatccga agaaccggag cgtcctccgg ctcccggacc acctcgaacg
     3001 actcgaccga agggtgcccg gcgatgatgt cggggggcgc gcctccgggc gggatgacga
     3061 cgaggcacgg gaagctccag acggcggcgt cgtcgacgag cgtccgcccc ggctcgggct
     3121 cgcccgggcg ggccacccag acgatcgggc cggggagctc ggagaggccc ggggccgatt
     3181 cgaacggcat cggctccacg ccgaagccgg gtccgcggag gttcgtcgtc acccagccga
     3241 cggcgtcggc gtcgccgacg aggcggacga cgaaatgccc cgacgcggcg gtcggggagt
     3301 acggcatcgg cgctgggacg gcgaacatcg cctcattctc gtccccctca cgcctcccgg
     3361 tccacatgcg aagataaagc gtgctccgaa aagctgcact cctcctcgcc tctctggtcg
     3421 cgtccggagc cttcgcggag gggccgcggc gcgaggcctc ctacgtcctc tccgaaggga
     3481 ggaccgtcgt ctcgcccgcg ggcgagcggg cgagcgccgt ccgcggcacg gtcgtcgtcc
     3541 gcggcgggac ggcgcgatgg gacctcgaga gcgggacctt cccgcgctcg acggcgagca
     3601 cgatcctcct cggcgatcgg ggcggctggc ttctcgagcg ggacgagagc gtggccgccc
     3661 gggcgaccgc ggaggacctc acggcgctct gggtgccggc cccgacggga gaggaagggc
     3721 cgttccggag ctccgtccgg ggactcgcga tccagccccc gacggccgcc cccgggccgg
     3781 tgttcgaggg gcgcccgacg aagcgccatc ggctgacggc ggactggagc ctggagacct
     3841 cgacgcccgg gcgcgtctcg acggcccgca cccgcctcgt cgccacggtc gtcgccgtcg
     3901 aggacgtcga tgaggaggtc ctctcgccgc tcgacggctt cgatcgactg ctcgacctgc
     3961 ccggccccgt gcgagaggag atcgcggccg cgctcggcgc gatccccggg tggccggtct
     4021 cggtcatcgt ggagacggaa tcggagcact cctccgagct ccctggaatc caggcggcgt
     4081 cgcccgaagg acgaagaccc ctgaagacgc gggtcgaggc cgtccggaag gtctcggccc
     4141 tcgtccttcg ccccgcgggg gaggcgagct cggccttcac gcttccggag aggacgcgcg
     4201 tcgtcgggat cgagcgcctc gtcaccccgc gcgagtcgct ccgttgacgc agcccggcgc
     4261 ggcaggcggc cttctcgccg ggctcgcgcc agaagaccgg ccgagggaga agctcgtcgc
     4321 gcgcggcgcc gaggcgctga ccgacgagga gctcgtcgcg attctcctcg gcacgggccg
     4381 ggcgggcgcc ggggtcctcg agacggcgcg ggaggtcctt cgcggaggcg gtcttcgcgg
     4441 attgctcgcc cggagcgaac ggggccgcgt gcgcgagctc gcacggggga tcggggtcgc
     4501 gaaggcgtgc cggatcgcgg cggcggccga gctggcgcgc cggctcgccc gcgccgaggt
     4561 ggcggagcgt ccgctcatcg acgcgccgga ggccgccggg gcgtacctcg cggcgaccct
     4621 cgccgcagag ctgcgggagg tgatgggggg gctcctcctc gacgcgaaga accggctgat
     4681 ccacgacgcc gtcgtgtttc gcgggggcgt cacctccgcg tcggtccagc cgggaccgct
     4741 cctccggatc gccgtcctgg aaggcgcggc ggccctcgtc ctctaccaca accacccgtc
     4801 cggcgacccg acgccgtcgc ccgaggaccg ggcgacgacg gcccgcttcg tcgcggccgg
     4861 ggaggccgtc ggcgtcccgg tccgcgacca cgtcgtcgtc gggcgggggt gctggttctc
     4921 gttccgccgg gaaggcctga tgtagacccg tcgaggccgc ggcggaggcc tgcggcacgg
     4981 gcccgcctga tatcctcgcc ggcccatgtc ccggtactac acgaccccga tctattacgt
     5041 caacgaccgt ccccacatcg gccacctcta cacgaccctc gtcaccgaca cgctgacccg
     5101 gttccatcgc ctccagggcg aggacgtacg gttcctgacc gggaccgacg agcacgggca
     5161 gaagatcgag aaggtcgcga agaaggaggg ggtcgccccg atcgccgtcg ccgaccggca
     5221 cgccccgacg ttccgagacc tctggaagcg cttcagcgtc gcgaacgacg acttcctccg
     5281 gacgacggag cctcgtcacc gcgtcggcgt gacggagatg atccgccgcc tcggggccgc
     5341 gggcgacctc tacgtcgccg ggcacgaggg gtggtactgc gtcggctgcg aggc
//
LOCUS       DAOTEZ010000302         5380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_10992_length_5380_cov_5.585164, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000302 DAOTEZ010000000
VERSION     DAOTEZ010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5380
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10992_length_5380_cov_5.585164"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="P5164_14375"
     CDS             complement(<1..361)
                     /locus_tag="P5164_14375"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRY45127.1"
                     /translation="MIGNRMFVAGVVATAALVALPVSAQSAQKVGVIEVQRIVQDSAV
                     GKESLARIQKVSAAKQEDLTKRQTELRDLEKRIQEQGKSLSEEAMEKLQKDYQQKALD
                     LKRFQDDAQRELEELQRR"
     gene            complement(745..3345)
                     /gene="bamA"
                     /locus_tag="P5164_14380"
     CDS             complement(745..3345)
                     /gene="bamA"
                     /locus_tag="P5164_14380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HRY45128.1"
                     /translation="MCIVPDATTGAPALPFGAWRPAGGARAAVTLAVLLGLAFPASAR
                     AQGEPPAAPAAPPATLDLRPGAAAPQAPASPLAGALPPAPELRTSPGADGRERIAEVA
                     VRGGRTVTAETIAFYLGVKVGDPYDVETLRRAFPKLWESGLFEDLRLERESAPGGARI
                     VAVVVERPRIGDLEFRGNKKLTTSQLKDKLKEGKVEIRVGSPLSLSDVSKGKGVLMEA
                     YRSDGFRSAVVDTALEPMGENEKRVVFLVDEGDKIKIESIDFTGNTVFSDRRLRFAMK
                     KTRQRNFYQFWDSKDVYNQANYEEDIESVKKVYQNAGYKDVVAKDPVIETFVVNPGET
                     RRDKVKRRARIEIPLVEGERFYFGDVKVEGTTVFPGDRLLQFFDWSPGKPLKRSALVD
                     GMKTLDEIYRGRGYIYAFMNPEYTERPGEGNLVDVVVRVTEGDMFRVGRVEFTGNKTT
                     KDKVLRREIPLFEGDVLDMESFKKGLLKLSQLGYFKIEEDPDFRVDPEKKVVDVTIKG
                     LDTNRNEIQFGAGYSQLDGVFGQFQFATRNFLGRGDTISVQFQRGGYSNFFDLSFVEP
                     WFLDKRMSIGGSIFNRSLEYLDVDQQTKGVNMSFGLGLGLFDGLSLFYGYTDTKSRYQ
                     VYPAAPPPGSGIPPAAYADYIGKTSAIAPGYRYDSRNDPFDPNRGRRFNFSTTIAGGP
                     LGGNFSYIKPVGGATSYFRTGRKSHFAVNVEGGFIRAYDGKEIPIFERFRIGGDRSVR
                     GFQYGSIYPLDDDDKAFFTDAGALLGGDKYFVFNAELVHAIAGPLKLVLFFDAGNAWL
                     EGQSFNPLKARASTGAELRMFLPIFQAPLRFIYGINLDPKTIKGPDGSPLNGGAEKSS
                     DFQFSIGTTF"
     gene            complement(3360..>5380)
                     /locus_tag="P5164_14385"
     CDS             complement(3360..>5380)
                     /locus_tag="P5164_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRY45129.1"
                     /translation="EEAIALIKEREASKQKKELPFLAEYARDLTALAAQGTFDPLVGR
                     DKEVERIIQILSRRTKNNPILLGEPGVGKTAIVEGLAQRIVEGAVPLFLASRKILAMD
                     LSLIVAGTKYRGQFEERLKGILKELRENQDLIIFIDEIHSLIGAGSAEGSLDAANILK
                     PALSRGEISCIGATTLKEYRKYIEKDRSLLRRFQAITVNPPSEEETLEILEGVRERYE
                     RFHRVRYAPDAVRTAVYQSSRYIPDRFFPDKAIDLLDEAGAKVKLRMTADTQNLRRLE
                     NEARQIVKDMKKAISDKDFEHAVSLREREVEIKEEIERTRNAQLDEGSWAEVVRGDIE
                     EIIASWTGIPVTSIQVEEAEKLLNMETTLRKRVIGQDRAIDAISRAIRRSRLGVKNPN
                     RPMGSFIFLGPSGVGKTEVARRLAEFLFDAQKALVRFDMSEYMEKHAVSKLIGSPPGY
                     VGHEEGGQLTERIRRQPYSVILLDEIEKAHPDIANLLLQILEDGQLTDAYGNTVDFKN
                     TLIIMTSNIGTKYLVTQARVGFGDGKNAPPAREVEEMVLKELRREFSPEFTNRIDEVI
                     VFNPLTTENLRAICRLLLDDVAQTLKHRGLELVADEKAVDWLLSMSGQDAHSGARPLR
                     RTIQRHVEDAVSELLIGAREQLECLEVTVIDSELKVLVRSTAALAAER"
BASE COUNT          788 a         1806 c         1830 g          956 t
ORIGIN      
        1 cgcggcgctg gagctcctcg agctcccgct gcgcgtcatc ctggaagcgc ttgaggtcga
       61 gggccttctg ctggtagtcc ttctgaagct tctccatcgc ctcttcggag agcgacttgc
      121 cctgctcctg gatccgcttc tcgaggtcgc ggagctccgt ctggcgcttc gtcaggtctt
      181 cctgcttggc ggccgagacc ttctgaatcc gggcgaggct ctccttgccc acggcggagt
      241 cctggacgat ccgctggacc tcgatgacgc cgaccttctg ggcggactgg gccgaaacgg
      301 ggagagcgac gagtgcggcc gtcgcgacga cgcccgcgac gaacatccgg ttcccgatca
      361 tctacttgac tccttcttcc gtgtctggcc ccgccggagg ccctccggag gggcgcgcga
      421 ttctactgca ccaaccgact ccgccaacct gcggatcggg gcccctcgaa ggctcctggc
      481 gccgtgagcg gtcctgccgc gcggaggggg tccgaggggg ccgggggagg ccatcgcgcc
      541 ttccccgctc aaagagctgc cggatcccgg cggtccgggt tcccccggta actccgtgcg
      601 agcccggatt tccgagattc ccgaaagccc cggaactggc cgggggtccg gggggccgcg
      661 cgaagctcag gccccccggg aagcgcgagc tgcctttccg cgcggagggg gtgaggggga
      721 aacccggcgg tccgggttcc ccctctagaa agtcgtcccg atggagaact ggaagtcgct
      781 gctcttctcc gcgccgccgt tgagcggcga cccgtcgggc cccttgatcg tcttgggatc
      841 gaggttgatc ccgtagatga agcgcagcgg cgcctggaag atcggaagga acatccgcag
      901 ctcggccccc gtcgaggcgc gcgccttgag cgggttgaag ctctgcccct cgagccaggc
      961 gttgccggcg tcgaagaaga ggacgagctt cagcggcccc gcgatggcgt ggacgagctc
     1021 ggcgttgaag acgaagtact tgtcgccgcc cagcagcgct cccgcgtcgg tgaagaacgc
     1081 cttgtcgtcg tcgtcgagcg gataaatgct tccgtactgg aagccgcgca ccgagcggtc
     1141 ccctccgatc cggaaccgct cgaagatcgg gatctccttg ccgtcgtagg cacggatgaa
     1201 cccgccttcc acgttcacgg cgaagtggga cttccggccc gtccggaagt acgacgtggc
     1261 cccgccgacc ggcttgatgt acgagaagtt cccgccgagc ggtccgcccg cgatcgtcgt
     1321 cgagaagttg aaccggcgcc cccggttcgg gtcgaacggg tcgttccggc tgtcgtaacg
     1381 gtagccgggc gcgatcgcgg aggtcttccc gatgtagtcg gcgtaggcgg cgggcggaat
     1441 gcccgagccc ggaggcggag cggccgggta gacctggtat cggctcttcg tgtccgtgta
     1501 cccgtagaag agcgacagcc cgtcgaacag cccgaggccg aggccgaagg acatgttgac
     1561 gcccttcgtc tgctggtcga cgtcgaggta ctcgagagag cggttgaaga tcgagccgcc
     1621 gatcgacatc cgcttgtcga ggaaccaggg ctccacgaag gagaggtcga agaagttcga
     1681 gtagccgccg cgctggaact ggacgctgat cgtgtcgccg cgcccgagga agttccgggt
     1741 cgcgaactgg aactgcccga agaccccgtc gagctgcgag tacccggcgc cgaactggat
     1801 ctcgttccgg ttcgtgtcga gccccttgat cgtgacgtcg acgaccttct tctccgggtc
     1861 gacgcggaaa tcggggtctt cctcgatctt gaagtagccg agctgggaga gcttgagaag
     1921 ccccttcttg aacgactcca tgtcgaggac gtcgccctcg aagaggggga tctcccgccg
     1981 gaggaccttg tccttcgtcg tcttgttccc ggtgaactcg acccggccca cgcggaacat
     2041 gtcgccctcg gtcacccgga cgacgacgtc gacgaggttc ccttcgcccg gccgctcggt
     2101 gtactccggg ttcatgaagg cgtagatgta gccccgtcca cggtagatct cgtcgagcgt
     2161 cttcataccg tcgacgagcg ccgaccgctt gagcggcttg cccgggctcc agtcgaagaa
     2221 ctggaggagg cggtccccgg ggaagaccgt cgtcccctcg accttgacgt cgccgaagta
     2281 gaagcgctcg ccttcgacga gcgggatctc gatccgggcc cggcgcttga ccttgtcgcg
     2341 gcgggtctcg ccggggttca cgacgaaggt ctcgatgacc gggtccttcg cgacgacgtc
     2401 cttgtacccg gcgttctggt agaccttctt gacggactcg atgtcctctt cgtagttcgc
     2461 ctggttgtag acgtccttcg agtcccagaa ctggtagaag ttccgctgcc gcgtcttctt
     2521 catcgcgaac cggaggcgcc ggtcggagaa gacggtgttg ccggtgaagt cgatcgactc
     2581 gatcttgatc ttgtcgccct cgtcgacgag gaagacgacg cgcttctcgt tctcccccat
     2641 cggctcgagg gccgtgtcga cgaccgcgga gcggaacccg tcgctccggt acgcttccat
     2701 caggacgccc ttccccttgg agacgtcgct gagggagagg ggcgacccga cgcggatctc
     2761 caccttcccc tccttcagct tgtccttcag ctgggaggtc gtcagcttct tgttcccgcg
     2821 gaactcgagg tcgccgatcc gggggcgctc cacgacgacc gcgacgatgc gcgccccgcc
     2881 cggggcgctc tcccgctcca ggcggaggtc ctcgaagaga cccgactccc agagcttcgg
     2941 gaaggcgcgc cggagcgtct cgacgtcgta cgggtcgccg accttcaccc cgaggtagaa
     3001 cgcgatcgtc tccgcggtga cggtccggcc gccgcgcacg gcgacctcgg cgatccgttc
     3061 cctgccgtcg gcccccggag acgtccggag ctccggagca gggggaaggg cccccgcgag
     3121 aggggaggcc ggagcctgcg gcgccgctgc ccccggccgg aggtcgagcg tggcgggagg
     3181 cgccgccgga gcggcaggcg gctccccctg cgcccgcgcc gaagcgggga acgcgaggcc
     3241 gaggaggacg gcgagggtca cggcggcgcg ggcgccgccc gcgggtcgcc acgctccgaa
     3301 cgggagcgcc ggggcgcccg tcgtcgcgtc agggacgatg cacaactcct gcacgtcgtt
     3361 taccgctcgg ccgccagcgc ggccgtagag cggacgagaa ccttcagttc gctgtcgatc
     3421 acggtgactt cgaggcactc gagctgctcc cgggcgccga taagcaattc ggataccgca
     3481 tcctccacgt ggcgttggat cgtccttcgg agcggccggg cgcccgagtg ggcgtcctgc
     3541 cccgacatcg agaggagcca gtcgaccgcc ttctcgtcgg cgacgagctc gaggccccgg
     3601 tgcttcaggg tctgggcaac gtcgtcgagg aggagccggc agatggcccg gaggttctcc
     3661 gtcgtgagcg ggttgaacac gatgacctcg tcgatccggt tcgtgaactc cggagagaac
     3721 tcgcggcgga gctccttgag gaccatctcc tccacctcgc gggcgggcgg cgcgttcttc
     3781 ccgtcgccga agccgacccg cgcctgcgtg acgaggtact tcgtcccgat gttcgaggtc
     3841 atgatgatca gcgtgttctt gaagtcgacc gtgttcccgt aggcgtcggt cagctgcccg
     3901 tcctcgagga tctggaggag caggttcgcg atgtcgggat gggccttctc gatctcgtcg
     3961 aggagaatga ccgagtaggg ctggcgccgg atccgctcgg tgagctggcc accctcctcg
     4021 tgcccgacgt agccgggcgg ggagccgatg agcttggaga ccgcgtgctt ctccatgtac
     4081 tcggacatgt cgaagcggac gagcgccttc tgggcgtcga agaggaactc cgcgagcctc
     4141 cgcgcgacct cggtcttccc gacgcccgaa gggccgagga atatgaacga gcccatcggc
     4201 cggttcgggt tcttcacgcc gagccgcgac cgccggatcg cgcgcgagat ggcgtcgatc
     4261 gcccggtcct gcccgatgac ccgcttgcga agcgtcgtct ccatgttcag gagcttctcg
     4321 gcctcctcga cctggatcga ggtgaccggg atgccggtcc agctcgcgat gatctcctcg
     4381 atatcgcccc ggacgacctc ggcccacgac ccctcgtcga gctgggcgtt ccgggtccgc
     4441 tcgatctcct ccttgatctc gacctcgcgc tcgcggagcg agacggcgtg ctcgaagtcc
     4501 ttgtcggaga tcgccttctt catatccttg acgatctggc gggcctcgtt ctcgaggcgc
     4561 ctcaggttct gcgtatccgc cgtcatgcgg agcttcacct tcgcgcccgc ctcgtcgagg
     4621 aggtcgatcg ccttgtcggg gaagaagcgg tcggggatgt agcggctcga ctggtagacg
     4681 gcggtccgca ccgcgtcggg ggcgtaccgc accctgtgga accgctcgta ccgctcgcgg
     4741 accccctcga ggatctcgag cgtctcctct tcgctcggcg ggttgaccgt gatcgcctgg
     4801 aagcgccgga ggagcgagcg gtccttctcg atgtacttgc ggtactcctt gagggtcgtc
     4861 gcgccgatgc aggagatctc cccccgcgag agcgccggct tgaggatgtt cgcggcgtcg
     4921 agcgagccct cggccgagcc cgccccgatc agggagtgga tctcgtcgat gaagatgatg
     4981 aggtcctggt tctcccggag ctccttcagg atccccttga gccgctcctc gaactgcccg
     5041 cggtacttcg tcccggcgac gatgagcgag aggtccatcg cgaggatctt ccggctcgcg
     5101 aggaagagcg gcaccgcgcc ctcgacgatc cgctgcgcga gcccttcgac gatcgccgtc
     5161 ttgccgacgc ccggctcccc gaggaggatc gggttgttct tcgtccggcg cgagaggatc
     5221 tggatgatcc gctcgacctc cttgtcgcgg ccgacgagcg ggtcgaacgt cccctgagcg
     5281 gccagcgccg tcaggtcgcg cgcgtactcg gcgaggaacg ggagctcctt cttctgcttg
     5341 gaggcctcgc gctccttgat gagggcgatc gcctcctccc
//
LOCUS       DAOTEZ010000303         5376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_11002_length_5376_cov_5.941176, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000303 DAOTEZ010000000
VERSION     DAOTEZ010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11002_length_5376_cov_5.941176"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="P5164_14390"
     CDS             complement(<1..848)
                     /locus_tag="P5164_14390"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HRY45130.1"
                     /translation="MKGPRERSHLHAAPVACAHCGLAVPEGMLRPGEEKQFCCSGCRQ
                     VHDLIRDWGYEKFYGLVEQQGGALEPARPTGRGFEDLDDPRALEGATEEAPGGRCRTR
                     LYLEGVHCAACVWLVEKLPEALDGVDSVRLNLANAVAEVTWRPERVKLSAVARALDRL
                     GYSPHVHRAGAARDARRAEDRAALVKLGVAVAGAMNLMFLHGALYAGEASGMASPYEA
                     FFRWVSLGIALPVLAFSARPFFQTAWAGLKSRFVHIDLPVAIGIAVAFAASAANVVRG
                     SGPVYF"
     gene            complement(845..1378)
                     /locus_tag="P5164_14395"
     CDS             complement(845..1378)
                     /locus_tag="P5164_14395"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HRY45131.1"
                     /translation="MKLRAFLSEYRWPLYLTGLLTMSLLAYAVLVFVATRPTAPRPIA
                     GFYEASQEWDADEAVLAASRALGWTVRYELPAGVPHVAGAPRPVDVHVADRDGQGVTG
                     LTGRLFALRAAEARRDPPGALVELPHLPGSYRALLRVDEPGAWDFRLDARRDGQRFVH
                     SARVVVGKDAPEGGSAR"
     gene            complement(1375..2844)
                     /gene="ccoG"
                     /locus_tag="P5164_14400"
     CDS             complement(1375..2844)
                     /gene="ccoG"
                     /locus_tag="P5164_14400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HRY45132.1"
                     /translation="MTAPAAAKEERERAAKPAGRERVFEVAPEEELLYSMSADGKRKF
                     IRPVLHRGRYWKVRRAMAWGLFVLFVALPLVPLGGHPAVHLDLGQRLFHVLGTTFHPT
                     DNVLLLLFGFGVIVTVFFVGSTFGRMWCGYACPQTVYLEFFFRPVELFLEGTPPAQRK
                     LNAAPMSARKAGIKTAKWAIWTLLALLLASTFVAYFVSWKGLLAGLASPGEATGALGT
                     VAFLTALMLFDFGWFRDQMCTVACPYGRLQSVMADPDTILVAYDAKRGDPKVKVKDRV
                     AGVAAGDCIDCRSCVNACPTGTDIRRGLQSECIGTAQCIDACDEVMLGQKKPIGLIKY
                     TSEREEAGGTRRLVRPRNLIYLALLTLAWGSLAFLLLTRADALVEIVRGGREPYRLLV
                     TGEVANQQRLRVTNQLDVPQSFSVEVLSPGGARLVLAGGPVDVPPEKVVTVNVVTTVP
                     AEVFRDGQATARYLVTSDRGFRKEVEFLLLGPWGKEAGR"
     gene            complement(2846..3433)
                     /locus_tag="P5164_14405"
     CDS             complement(2846..3433)
                     /locus_tag="P5164_14405"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRY45133.1"
                     /translation="MAEYEDKVLHELDGIKEYDNPLPGWLMAIWWGAIAFGVLYLAFY
                     ALRFGERSMESEYRAETEKALASVQAHFDANPLVAPTAAELLAGAADPVVLDRGAGRF
                     AKSCASCHGEKAQGLIGPNLTDDRWIHGGSVEQVFQSIVKGWPAKGMPPWGRAIPPEE
                     ISALVSYVRSLQGSAPANAKPAEGDPFPPEPIPGR"
     gene            complement(3446..3649)
                     /locus_tag="P5164_14410"
     CDS             complement(3446..3649)
                     /locus_tag="P5164_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45134.1"
                     /translation="MLQELARESAAPGWAIASMLFFLGVWLVLAVRVVRARPDALAAQ
                     ARLPLADDEAASPALAAEPGHGA"
     gene            complement(3655..>5376)
                     /gene="ccoO"
                     /locus_tag="P5164_14415"
     CDS             complement(3655..>5376)
                     /gene="ccoO"
                     /locus_tag="P5164_14415"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HRY45135.1"
                     /translation="PYSWLGSYSAYAGVKDALMQWWYGHNAVAFFLTTPYLGLMYYYL
                     PKAAERPVFSYKLSIMHFWSLVFVYIWAGPHHLHYTALPEWASTLGMLFSLILWMPSW
                     GGMVNGLLTLRGAWNKLRDDPVLKFLVVAVTYYGMSTFEGPMMSIKSVNAVSHYTDWT
                     IGHVHAGTLGWNSFLSFGILYWIVPRLWKTELFSKRLATAHFWIGTIGLILYQVSMWV
                     AGITQWAMWRAFEPDGRLVYPDFIETVLRIVPMYWVRLVGALLFFAGLLVMLYNLVRT
                     MRSAPAGFDEEPEVKAPPLVVDRSAPGATTAPGTWDHAFFRFQHVIRHGVHRAIEGRV
                     VLLTVLTALALSVGSLVEAIPLFFDESNVKTIASVKPYTPLELLGRDVYIREGCYNCH
                     SQMVRPFRHETERYGEYSKAGEFVYDHPFQWGSKRTGPDLHRVGGKYPSLWHVRHMDQ
                     PRSTTPGSVMPRYPHLLKDPIDDSEVEAKMRAMRAVGVPYGDGEIATAKASIAAQAQA
                     IATEVEAQQGPPGLAGREIMALTAYLQRLGTDIRWRRPLVQPPLALPAAPSAGPATAA
                     APVAAER"
BASE COUNT          817 a         1809 c         2044 g          706 t
ORIGIN      
        1 tcgaagtaga ccgggccgct cccgcggacg acgttcgcgg cgctcgcggc gaacgcgacg
       61 gcgatcccga tcgcgaccgg gaggtcgatg tggacgaagc gggacttcag ccccgcccac
      121 gcggtctgga agaacggccg cgccgagaag gcgaggacgg gaagcgcgat gccgagggag
      181 acccagcgga agaacgcctc gtagggggag gccatgccgg acgcctctcc ggcgtagagc
      241 gccccgtgga ggaacatcag gttcatcgcc ccggccacgg cgacgccgag cttcacgagg
      301 gcggcgcggt cttcggcgcg ccgcgcgtcg cgggcggcgc cggcccggtg gacgtgcggc
      361 gagtagccga gccggtcgag ggcgcgggcg acggccgaga gcttcaccct ctccggacgc
      421 caggtcacct cggcgaccgc gttcgcgagg ttcagcctca cggagtcgac gccgtcgagc
      481 gcctcgggaa gcttctcgac cagccagacg caggcggcgc agtggacccc ttcgaggtag
      541 aggcgggtcc ggcagcgtcc cccgggcgcc tcctcggtcg cgccttcgag cgcgcgcggg
      601 tcgtcgaggt cctcgaaccc gcgcccggtc gggcgggccg gctcgagcgc gcccccctgc
      661 tgctcgacga ggccgtagaa cttctcgtag ccccagtcgc ggatcaggtc gtggacctgc
      721 cggcagccgc tgcagcagaa ctgcttctcc tcgccgggac ggagcatccc ctcggggacc
      781 gcgaggccgc aatgcgcgca cgccacgggg gcggcgtgca ggtgcgagcg ctcgcgaggt
      841 cccttcaccg ggcgctccct ccctcggggg cgtccttccc gaccacgacg cgcgccgagt
      901 ggacgaagcg ctggccgtcg cggcgcgcgt cgaggcggaa gtcccaggcg cccggctcgt
      961 cgacgcgcag gagcgcgcgg taggagccgg ggaggtgggg cagctcgacg agggcgccgg
     1021 gcgggtcgcg acgcgcctcg gccgcgcgca gggcgaagag gcggccggtc agccccgtca
     1081 cgccctgccc gtcgcggtcg gcgacgtgga cgtcgacggg gcggggcgct ccggcgacat
     1141 gcggcacgcc ggctggcagc tcgtacctca ccgtccagcc gagcgcgcgg ctcgccgcca
     1201 ggacggcctc gtccgcatcc cactcctggg aggcctcgta gaagccggcg atcgggcgcg
     1261 gcgccgtcgg ccgcgtcgcc acgaagacga ggaccgcgta ggcgaggagc gacatcgtca
     1321 ggaggcccgt caggtagagg ggccagcggt actcggagag gaacgcccgg agcttcatcg
     1381 tccggcctcc ttgccccagg gcccgaggag gaggaactcg acctccttcc ggaagccccg
     1441 gtccgacgtg acgaggtacc gggccgtcgc ctgtccgtcg cggaagacct cggccgggac
     1501 ggtcgtcacg acgttgacgg tgacgacctt ttccgggggg acgtcgaccg gcccgccggc
     1561 caggacgagc cgcgcgccgc cgggggagag gacctcgacg gagaagctct gcggcacgtc
     1621 gagctggttc gtcacgcgga gccgctgctg gttcgcgacc tcgccggtga cgaggagccg
     1681 gtacggctcg cgtccgcccc ggacgatctc gacgagagcg tcggcgcggg tcaggaggag
     1741 gaaggcgagg gagccccagg cgagcgtcag gagcgcgagg tagatcaggt tccggggcct
     1801 cacgaggcgc cgcgtccctc ccgcctcctc gcgctcggag gtgtacttga tcagcccgat
     1861 cggcttcttc tggccgagca tcacctcgtc gcaggcgtcg atgcactggg ccgtcccgat
     1921 gcattccgac tggaggccgc ggcggatgtc ggtgccggtc gggcaggcgt tcacgcagct
     1981 ccggcagtcg atgcagtcgc ccgcggcgac gcccgcgacc cggtccttca ccttcacctt
     2041 cgggtcgccc cgcttcgcgt cgtaggcgac caggatcgtg tcggggtcgg ccatgacgct
     2101 ctggagccgg ccgtagggac aggcgacggt gcacatctgg tcgcggaacc agccgaagtc
     2161 gaagagcatc agcgccgtga ggaaggcgac cgtcccgagc gctccggtcg cctcgccggg
     2221 cgaggcgagg cccgcgagga gccccttcca gctgacgaag taggcgacga acgtggaggc
     2281 gaggaggagc gcgaggagcg tccagatcgc ccacttggcc gtcttgatcc ccgccttgcg
     2341 cgcgctcatc ggcgccgcgt tcagcttgcg ctgcgcgggc ggcgttccct cgaggaagag
     2401 ctccacgggg cggaagaaga actcgaggta gacggtctgc gggcaggcgt agccgcacca
     2461 catccggccg aacgtcgagc cgacgaagaa gaccgtgacg atgacgccga atccgaagag
     2521 gaggaggagg acgttgtcgg tcgggtggaa cgtcgtcccg aggacgtgga agagccgctg
     2581 gccgaggtcg aggtggacgg ccgggtgccc gccgagcggg acgagcggca gcgcgacgaa
     2641 gaggacgaag agtccccacg ccatcgcccg ccggaccttc cagtagcggc cgcggtggag
     2701 gacgggccgg atgaacttcc gcttcccgtc cgccgacatg ctgtagagga gctcctcctc
     2761 gggagcgacc tcgaagacgc gctcccgtcc cgccggcttc gcggcgcgct ctctctcctc
     2821 tttcgcggcg gcgggcgcgg tcacgtcagc gtcccgggat cggctcgggc gggaacgggt
     2881 cgccctcggc gggctttgcg ttcgcggggg cggagccctg gaggctcctc acgtacgaga
     2941 cgagcgccga gatctcctcg ggcgggatcg cccggcccca gggcggcatc cccttggcgg
     3001 gccacccctt cacgatcgac tggaagacct gctcgacgga gccgccgtgg atccagcggt
     3061 cgtcggtcag gttcgggccg atcagcccct gagccttctc tccgtggcag gaggcgcacg
     3121 acttcgcgaa acgcccggct ccccggtcga ggacgaccgg gtccgcggcg ccggcgagga
     3181 gctcggcggc ggtcggcgcc acgagcgggt tcgcgtcgaa gtgcgcctgg accgaagcga
     3241 gcgccttctc cgtctccgcg cggtactccg actccatcga ccgctcgccg aagcggaggg
     3301 cgtagaaggc gaggtagagg actccgaaag cgatcgcccc ccaccagatg gccatgagcc
     3361 agccggggag tgggttgtcg tactccttga tgccgtccag ctcgtgcagg accttgtctt
     3421 cgtattcggc catcgtcccc tccggtcagg ccccgtgacc cggctctgcg gcgagcgcgg
     3481 gggaggcggc ctcgtcgtcg gccagcggca ggcgggcctg cgctgcgagg gcgtcgggcc
     3541 gggcgcggac gacgcggacc gcgaggacga gccagacgcc gaggaagaag agcatcgagg
     3601 cgatcgccca ccccggggcg gcgctctcgc gggcgagctc ctggagcatc gcggctacct
     3661 ctcggccgcg acgggcgcgg cggccgtcgc ggggccggcg gacggcgccg ccgggagcgc
     3721 gagcggcggc tggaccagcg gccggcgcca gcggatgtcg gtcccgagcc tctggaggta
     3781 ggcggtcagc gccatgatct cgcggccggc gaggccgggc gggccctgct gcgcctccac
     3841 ctccgtggcg atcgcctgcg cctgcgcggc gatcgaggcc ttcgccgtcg cgatctcgcc
     3901 gtcgccgtac gggacgccga cggcgcgcat cgcgcgcatc ttcgcctcga cctccgagtc
     3961 gtcgatcggg tccttcagga ggtgagggta gcgcggcatg acggagccgg gcgtcgtgga
     4021 tcggggctgg tccatgtggc ggacgtgcca gagcgacggg tacttcccgc cgacgcggtg
     4081 gaggtcgggc cccgtccgct tcgagcccca ctggaacgga tggtcgtaga cgaactcgcc
     4141 ggccttggag tactcgccgt accgctccgt ctcgtgccgg aacggccgga ccatctggga
     4201 gtggcagttg tagcagccct cgcggatgta gacgtcgcgg ccgaggagct cgagcggcgt
     4261 gtagggcttc acgctcgcga tcgtcttcac gttgctctcg tcgaagaaga gcgggatcgc
     4321 ctcgacgagg gagccgacgg agagcgcgag ggccgtgagg accgtcagga ggacgacgcg
     4381 cccctcgatg gcgcggtgga cgccgtgccg gatcacgtgc tggaagcgga agaaggcgtg
     4441 gtcccacgtc ccgggggccg tcgtcgcgcc gggggccgag cggtcgacga cgagcggcgg
     4501 ggccttcacc tcgggctcct cgtcgaagcc ggcgggagcg ctcctcatcg tccggacgag
     4561 gttgtagagc atgacgagga ggcccgcgaa gaagaggaga gcgccgacga ggcggaccca
     4621 gtacatcggg acgatccgga ggacggtctc gatgaagtcg gggtagacga ggcggccgtc
     4681 gggctcgaag gcgcgccaca tcgcccactg ggtgatgccg gcgacccaca tcgagacctg
     4741 gtagaggatc agcccgatcg tcccgatcca gaagtgggcc gtggcgagcc tcttcgagaa
     4801 gagctccgtc ttccagaggc gggggacgat ccagtagagg atcccgaacg agaggaacga
     4861 gttccagccg agcgtcccgg cgtggacgtg gccgatcgtc cagtcggtgt agtgcgagac
     4921 cgcgttcacc gacttgatcg acatcatcgg cccctcgaac gtcgacatgc cgtagtaggt
     4981 cacggcgacg acgaggaact tcaggaccgg gtcgtcgcgg agcttgttcc acgccccgcg
     5041 gagcgtcagc aggccgttca ccatgccgcc ccaggagggc atccagagga tgagcgagaa
     5101 gagcatcccg agggtggaag cccactcggg gagcgccgtg tagtggaggt ggtgcggccc
     5161 cgcccagatg tagacgaaga ccagcgacca gaagtgcatg atcgagagct tgtagctgaa
     5221 gaccggacgc tcggccgcct tcggcaggta gtagtacatg aggccgaggt agggcgtcgt
     5281 caggaagaag gcgacggcgt tgtgcccgta ccaccactgc atcagggcgt ccttgacgcc
     5341 cgcgtaggcc gagtagctcc cgagccagga gtaggg
//
LOCUS       DAOTEZ010000304         5375 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_11006_length_5375_cov_4.909211, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000304 DAOTEZ010000000
VERSION     DAOTEZ010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11006_length_5375_cov_4.909211"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..1193)
                     /locus_tag="P5164_14420"
     CDS             complement(222..1193)
                     /locus_tag="P5164_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HRY45136.1"
                     /translation="MLPSFRYVRPRTVSEAVKALAAEGARAHAGGTDLLGCLRDGVFD
                     AKSVVSLSGLDDLKGISPTKDGGLKIGALTTLAEVARHPGIAKGWPALAQGAAAAASP
                     QLRNQGTLGGNLCQRPRCWYFRGDFDCARKGGEICYAVEGENTYHAIFGGSGCFIVHP
                     SDTASPLVALRATVKVVGPKGARSLPVEKLFVLPAVDHTRETILAPGEVLTEVLLPPP
                     LEGAKGLYRKVRSRGAWDFALGGVALALSVKDGKVASAGVVLSGVAPTPWRVPEVEKL
                     LVGKALDAKLAAEAAELAVKGAEPMSGNDYKVPLVKGAVEEALLSLA"
     gene            complement(1198..3495)
                     /locus_tag="P5164_14425"
     CDS             complement(1198..3495)
                     /locus_tag="P5164_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRY45137.1"
                     /translation="MTDLLYWLQGPVPETEPPTAEMPPWGETKVVGKPVPRVDAYERV
                     SGSAVYPLDFALPDMLHAAILRSPHAHARVKAIDTSAAEKMPGVRAILTAFSPGADVP
                     WYRSRGRFYSKLFDERVRHEGDEVAAVAAETRAQAFDALKAIRVEYEEFAFVLDPRES
                     LAPDAPLVHDGGNRYGEPARYERGDLAAGFAAADAVVEATYSTACEIHAPMEVHGSVA
                     KWDGNRLTIWDSTQGVFVVQQMVAGALKLPLANVRVIGHYMGGGFGSKLEAGKYTVIA
                     ALLAKTTARPVKLFLTREEVFLNAGNRPANTMKVKIGAKKDGALTAIEFSSIGSGGAY
                     PNGADTSFLATDLYTCPNVAAVDENVAINAGRARAMRAPGFPQGAWALEQAMDELALK
                     LGMDPVALRLANVPKVSQRRGNLPYTSTGLAECLRDGAKAFGWETTRAAKKDDGPIRK
                     GYGVAAGMWGYGGGPPARVVVKLFADGSVNVNAGAADIGTGTKTVMAQTVAEELGVPL
                     EKIQIEHADTGTTQYSGPSGGSKTVPSDMPAARAAAVEVRTKLLAGAAEELKVPAAEL
                     KLEGADVVSTKDPAKKLAVTEVPALQRNQVIVGVGTRGPNVEGKVISMFAAHFAEVEV
                     NVRTGEVSVTKLVAAQDSARVMNRLTFECQVQGGLVMGLGLGRTEERVLDRQTGKMVN
                     ANWHDYKIPTALDVPVAQEVLSAELVDTECNTAGAKGVGEPATIPTAAAIANAVRDAV
                     GLRVHDSPITPPKLAALLAAAGKKG"
     gene            complement(3500..4138)
                     /locus_tag="P5164_14430"
     CDS             complement(3500..4138)
                     /locus_tag="P5164_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRY45138.1"
                     /translation="MADGKDDRNDLDTNGGVSRRGFLGSVVPAAVGVAVAREALSAPA
                     AAPAVAELPAGEPAKLTLVVNGRTHKVLVEPRDTLLSVLREKLGLTGTKPGCERGECG
                     ACTVLIDGVARYSCLTLALEADGKKVTTVEGLMEGEELGPVQKAFVEEDAFQCGYCTP
                     GQVMAAEGLLRENPEPGLDEIRHGMSGNLCRCGAYAHIFKAVDKAARFRKER"
     gene            complement(4339..>5375)
                     /locus_tag="P5164_14435"
     CDS             complement(4339..>5375)
                     /locus_tag="P5164_14435"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose PTS transporter subunit EIIB"
                     /protein_id="HRY45139.1"
                     /translation="LFKMWGLPAAALAIWRSAREDRRARVGAVMLSAAATSFLTGVTE
                     PVEFAFLFLAPFLYALHALLAGLSYLVAELAGMRLGFTFSHGLFDLVLYWPMGTRPWL
                     VPLLGPLWALAYYALFRAAILRFDLRTPGREEPSASAAEPAAERPSAGAGEPSGIAER
                     LVAAFGGALNIESLDACITRVRVTVRDPARIDEGALLTLGAAGVVVLGNGVQAVFGTR
                     SENLKTAMEGVLEGRGGLTAREPSTREGAASPDVAARGRRLLEALGGPSNLRSVEACA
                     RTRVRVVLADPARVDEGRVRAAGANGVMRLPGGVVHVLAGDEAAELAAALGRQSEEAS
                     TFDPGEPKGA"
BASE COUNT          708 a         2014 c         1908 g          745 t
ORIGIN      
        1 ctcggctacc tcgcgctgct cctcctcgaa cggtgggagg cgacggtcgc cgccctgcgc
       61 gccctcggcc tcgccctcgt ccgccgcccg gccctcgcgc gcctccgcgc cgagcgcgac
      121 gccctcgtcg ccgagatggc cgccctcgac gcagactgta gagtgtagac cggaccccca
      181 cctacgaagg ggtcaggtct accatctaca gtctgcgggg atcaggcgag cgagaggagg
      241 gcctcctcca cggcgccctt cacgagcggg accttgtagt cgttgcccga catcggctcg
      301 gcccccttca cggcgagctc ggccgcctcg gcggcgagct tcgcgtcgag ggccttcccg
      361 acgaggagct tctcgacctc cgggacgcgc cacggcgtcg gggcgactcc cgagaggacg
      421 acgccggccg aggcgacctt cccgtccttc acggagaggg cgagggcgac gccgccgagg
      481 gcgaagtccc atgccccgcg cgaccggacc ttgcggtaga ggcccttcgc tccttcgagc
      541 ggcggcggga ggaggacctc ggtgaggacc tcgcccggcg cgaggatcgt ctcgcgggtg
      601 tggtcgaccg ccggaaggac gaagagcttc tcgacgggga ggctgcgagc ccccttcggt
      661 ccgacgacct tcaccgtggc ccggagcgcg acgagcggag aggcggtgtc ggaaggatgg
      721 acgatgaagc agccggagcc gccgaagatg gcgtggtagg tgttctcccc ctccacggcg
      781 tagcagatct cgcccccctt gcgggcgcag tcgaagtcgc cgcggaagta ccagcagcgc
      841 gggcgctggc agaggttccc gccgagcgtc ccctggttgc gaagctgcgg gctcgcggcg
      901 gcggcggcgc cctgtgcgag ggcgggccac cccttcgcga tcccgggatg ccgggcgacc
      961 tcggcgagcg tcgtcagcgc gccgatcttc aggcccccgt ccttcgtcgg cgagatccct
     1021 ttcaggtcgt cgaggcccga gaggctgacg acggacttcg cgtcgaagac gccgtcccga
     1081 aggcagccga ggagatccgt gccgccggcg tgagcgcggg ccccctcggc cgcgagcgcc
     1141 ttcacggcct cggagacggt gcggggacgg acgtagcgga agctcggaag catcgcgtca
     1201 ccccttcttc ccggccgcgg cgaggagcgc ggcgagcttc ggcggcgtga tcggagagtc
     1261 gtggacccgg aggccgacgg cgtcgcggac cgcgttcgcg atcgccgcgg cggtcgggat
     1321 cgtcgccggc tcgccgaccc ccttggcgcc cgcggtgttg cactcggtgt cgacgagctc
     1381 ggcggagagg acctcctgcg ccaccgggac gtcgagcgcc gtcgggatct tgtagtcgtg
     1441 ccagttcgcg ttgaccatct tcccggtctg ccggtcgagg acccgctcct cggtccggcc
     1501 gaggccgagg cccatcacga gcccgccctg gacctggcac tcgaacgtga ggcggttcat
     1561 gacgcgcgcg ctgtcctggg cggcgacgag cttcgtgacg gacacctcgc ccgtccgcac
     1621 gttcacctcg acctcggcga agtgcgctgc gaacatcgag atgaccttcc cctcgacgtt
     1681 cggcccgcgc gtcccgacgc cgacgatcac ctggtttcgc tggagagccg gcacctcggt
     1741 gacggcgagc ttcttcgcgg ggtccttcgt cgagacgacg tcggctccct ccagcttcag
     1801 ctccgcggcg gggaccttca gctcctccgc cgccccggcg aggagcttcg tcctcacctc
     1861 gaccgccgcg gcgcgcgcgg ccggcatgtc ggacggcacg gtcttcgatc cgccgctcgg
     1921 gccgctgtac tgcgtcgtcc cggtgtcggc gtgctcgatc tggatcttct cgagcgggac
     1981 gcccagctcc tcggcgacgg tctgggccat cacggtcttc gtgccggtgc cgatgtcggc
     2041 ggccccggcg ttcacgttga cgctcccgtc ggcgaaaagc ttcacgacga ccctcgccgg
     2101 agggccgccc ccgtagcccc acatcccggc cgcgacgccg taccccttgc ggatcggccc
     2161 gtcgtccttc ttcgccgccc gggtcgtctc ccacccgaac gccttcgcgc cgtcacggag
     2221 gcactccgcg aggccggtgg aggtgtacgg gaggttgccg cgccgctggc tcaccttcgg
     2281 gacgttcgcg aggcggagcg cgaccgggtc catcccgagc ttcaacgcca gctcgtccat
     2341 cgcctgctcg agcgcccagg ccccctgcgg aaagccgggg gcgcgcatcg cgcgggcccg
     2401 ccccgcgttg atcgcgacgt tctcgtccac ggccgcgacg ttggggcagg tgtagaggtc
     2461 cgtcgcgagg aacgacgtgt cggcgccgtt cggataggcg ccgcccgacc cgatcgagga
     2521 gaactcgatc gccgtcaggg cgccgtcctt cttcgcgccg atcttcacct tcatcgtgtt
     2581 cgcggggcgg ttccccgcgt tcaggaagac ctcctctcgc gtcaggaaga gcttcacggg
     2641 gcgggcggtc gtcttcgcga ggagcgccgc gatgaccgtg tacttccccg cctcgagctt
     2701 gctgccgaag ccgcccccca tgtagtgccc gatcacgcgg acgttcgcga gcgggagctt
     2761 cagcgcgccc gcgaccatct gctggacgac gaagacgccc tgggtcgagt cccagatcgt
     2821 caggcggttc ccgtcccact tcgcgacgga gccgtggacc tccatcggcg cgtggatctc
     2881 gcaggccgtc gagtacgtcg cctcgacgac ggcatcggcc gccgcgaacc ccgccgcgag
     2941 gtcgccccgc tcgtaccggg ccggctcgcc gtagcggttc ccgccgtcgt ggacgagcgg
     3001 ggcgtccggg gcgagcgact cgcgcgggtc gaggacgaac gcgaactcct cgtactcgac
     3061 gcggatcgcc ttcagcgcgt cgaacgcctg ggcgcgcgtc tccgcggcga ccgcggcgac
     3121 ctcgtccccc tcgtggcgca cccgctcgtc gaagagcttc gagtagaacc gcccgcggct
     3181 ccggtaccac ggcacgtcgg cgccgggaga gaacgcggtg agaatcgcgc ggacccccgg
     3241 catcttctcg gccgcgctcg tgtcgatcgc cttcacccgg gcgtgcgcgt ggggggagcg
     3301 gaggatcgcg gcgtggagca tgtcggggag ggcgaaatcg agcgggtaga cggccgagcc
     3361 gctgacgcgc tcgtacgcgt cgacccgcgg gacgggcttc cccacgacct tcgtctcgcc
     3421 ccacggtggc atctcggccg tcggcggctc ggtctcgggg acggggccct ggagccagta
     3481 gaggaggtcc gtcacggcgt cacctctcct tccggaaccg ggcggccttg tcgaccgcct
     3541 tgaagatgtg ggcgtaggcg ccgcaccggc agaggttgcc gctcatgccg tggcgaatct
     3601 cgtcgagccc gggctcgggg ttctcgcgca gcagcccctc cgcggccatc acctggcccg
     3661 gcgtgcagta gccgcactgg aaggcgtcct cctcgacgaa ggccttctgg acgggaccga
     3721 gctcctcccc ctccatcagc ccctcgaccg tcgtcacctt cttcccgtcg gcctcgagcg
     3781 cgagcgtgag gcaggaatag cgcgccacgc cgtcgatcag gaccgtgcac gcgccgcact
     3841 cgccccgctc gcagcccggc ttcgtcccgg tgaggccgag cttctcgcgg aggacggaga
     3901 ggagcgtgtc gcgcggctcg acgaggacct tgtgcgtcct cccgttcacg acgagcgtca
     3961 gcttcgccgg ctcgccggcg gggagctccg cgacggccgg cgccgccgcc ggtgcggaga
     4021 gcgcttccct cgccaccgcg acgccgaccg ccgccgggac cacggaaccg aggaagcccc
     4081 ggcgggagac gccgccgttc gtgtcgaggt cgttccggtc gtccttgccg tcggccatgc
     4141 gtcctccccg gggccgggac tcggcgccgc gcggcgtctc cggcccgatc gggcagttta
     4201 cgacgtggcg gagcgccgtt ccgcgtcgcg gaagatgcga cgacctctcg aaagtcatcc
     4261 tccgcaggat cgtccgcacg cacggcgggt tcccctccga gcccgggccc cggcccgccc
     4321 cccccccgac gagaggacct acgccccctt cggctcaccg ggatcgaagg tgctggcctc
     4381 ctccgactgc cggccgagag ccgccgcgag ctccgccgcc tcgtcccccg cgaggacgtg
     4441 gacgacgccg ccagggaggc gcatgacccc gttcgcgccg gcggcccgga ctctgccctc
     4501 gtcgacgcgc gccgggtcgg cgaggacgac gcgcacgcga gtccgcgcgc acgcctcaac
     4561 cgaccggagg ttcgacggcc ctccgagagc ctcgaggagc cggcgcccgc gcgcggcgac
     4621 gtcgggggaa gcggccccct cccgggtcga cggctcccgc gccgtcaggc ccccgcgccc
     4681 ttcgagcacc ccttccatgg ccgtcttgag gttctccgag cgcgtcccga agaccgcctg
     4741 gaccccgttc ccgagaacga cgaccccggc cgcgccgagc gtgaggagcg ccccctcgtc
     4801 gatccgggca ggatcgcgca ccgtcacgcg gacgcgcgtg atgcacgcat cgaggctctc
     4861 gatgttgagg gcgcccccga aggcggcgac gagcctctcg gcgatccccg acggctcgcc
     4921 cgcccccgcg gacggccgct cggccgcggg ctccgccgcg gaggcggagg gctcctcccg
     4981 tcccggagtc cgcaggtcga atcggaggat cgccgcccgg aagagcgcgt agtaggcgag
     5041 ggcccagagc ggcccgagga gcggcacgag ccagggacgc gtccccatcg gccagtagag
     5101 gacgaggtcg aacaggccgt gcgagaacgt gaagccgagc ctcatccccg cgagctccgc
     5161 gacgaggtag gagagccccg cgaggagggc gtgaagggcg tagaggaacg gcgcgaggaa
     5221 gaggaacgcg aactcgaccg gctcggtcac gcccgtcagg aacgaggtcg cggccgcgga
     5281 gagcatcacc gcccccacgc gcgcgcggcg gtcctcgcgg gcggagcgcc agatcgcgag
     5341 cgccgcggcg ggaaggcccc acatcttgaa gagga
//
LOCUS       DAOTEZ010000305         5361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_11049_length_5361_cov_5.431398, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000305 DAOTEZ010000000
VERSION     DAOTEZ010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5361
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11049_length_5361_cov_5.431398"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /gene="asnS"
                     /locus_tag="P5164_14440"
     CDS             <1..984
                     /gene="asnS"
                     /locus_tag="P5164_14440"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRY45140.1"
                     /translation="PKEHGVDFLMSQRHLWLRSAKQVAILRVRSEVESAIHDFYHSRG
                     YTRVDSPILTPNACEGTSNLFETQYTEEEKAYLSQSGQLYLEPAAAALGKVYCFGPTF
                     RAEKSKTRRHLREFWMVEPEVAFLEIDGLKDLAEEFVKELTARVLDRRKEDLKRLERD
                     TTVLEATVAKPFPRIDYRDAIGILEKKGFPVKFGDDLGGDEETALTEGFDTPVMVTRY
                     PAAIKSFYMQPDPNDPEVVLGLDMLAPEGYGEIIGGSQRIHDHDLLLERIRQHELPIE
                     AFQWYLDVRKYGSFPHSGFGMGLERFTAWMCGVPHLRECIPYPRMLYRIYP"
     gene            1073..3130
                     /gene="aroF"
                     /locus_tag="P5164_14445"
     CDS             1073..3130
                     /gene="aroF"
                     /locus_tag="P5164_14445"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRY45141.1"
                     /translation="MTDLPNVRAALDRIDAALLDLLAERAAVVDEVAALKSREHALLL
                     RDLERERDLLSKVGRDAQRRGLNAFHVVKIFRDVVEASVRQQESRLTRAANAIDEEPA
                     LLRVAFQGTEGAYSHLAGKKFFADRAEEPIFVGYRSFRQAVEAVEREECEFAILPIEN
                     SVAGAINETYTLLGTSKLSIVGEEVWPVEHCLLGLSKVPLARVRRVFSHPQALLQCSE
                     FLESLAGATAESWFDTAGSVARVKELGSEENAAIASEEAGERHGLVVLRRSIANQRNN
                     QTRFVVVHRRPFRFDPRIPCRTSLLLVTDHREGALERCLAELAKAGVNLTKLESRSMP
                     ATPWEYQFYVDVEGNVDDPRLAEALEGVGRNARYLRVLGCYPRKADPSREVPKDVDLL
                     TEAAPAEPWPAGTPVPVESPVAYPLAARRATDPGARTPVKVGDVVFGEGFVVIAGPCA
                     VENEAQLFEAARVVREGGGRVLRGGVFKPRTSPYSFQGLGIPGLDLLCRAGLEYHLPV
                     VTEVVAPEDVEKVAEFADMLQVGARNMQNFALLKAVGQSSRPVLLKRGMMSSVEELLL
                     AAEYVLSAGNRAVVLCERGIRTFETSTRNTLDLGAVQVLKARTHLPVIVDPSHAMGVT
                     AFVAPMARAALAAGADGVIVEVHPHPEEALCDGPQALTPELFTAMMGDLRRMAQALGV
                     KMA"
     gene            3228..4580
                     /gene="rimO"
                     /locus_tag="P5164_14450"
     CDS             3228..4580
                     /gene="rimO"
                     /locus_tag="P5164_14450"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRY45142.1"
                     /translation="MPLKLPSVGIVSLGCPKNLVDTEVMLGYLKREGLELGDPDTSRV
                     VIVNTCGFIDQAKEESVNAVLEQVRRKEAGEVDRVVVAGCMVQKYGAELAAEIPEVDH
                     FLGLDELEKAPAAALGLPSLARFAAKPLATRLYDDLAPRVLTGRRGYAYLKVAEGCNN
                     PCTFCTIPQMRGLQRSRSIESLVREAQGLEAQGVSELVLISQDTTRYGEDLGLGREGL
                     STLVAALLRETGFPWVRFLYAYPKTLHDSVLERMAAEPRFVSYVDVPLQHVSRSVLAG
                     MRRGGDPESCLRLVERLRAAVPGIALRSTFIAGFPGEGEAEFAELLAFVKEAGLDHVG
                     VFPYSPEPGSGAEGLGDPVPAPVKGERCAAVMEAQREISRRKNRELRGKVHDAILEGA
                     SPESEMLLEGRLARQAPEIDGKVLVNDVPEGFVPRPGAMVRVRVTGAHDYDLVGRVVP
                     "
     gene            4594..>5361
                     /gene="ribF"
                     /locus_tag="P5164_14455"
     CDS             4594..>5361
                     /gene="ribF"
                     /locus_tag="P5164_14455"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HRY45143.1"
                     /translation="MNVYPDPLRRSDLPRGGVVTIGNFDGVHLGHQAMLQAVVARARE
                     LDAPALVLTFDPHPTTVLHPQRAPKLIQTLRQREEAIAALGVDGLVVVPFTRDFAAMP
                     AEDFVRELLGRSLGVREAHVGAGFAFGCGKRGNVELLSRLGEEAGIRVATLDVVSEGD
                     APVSSTRIRSLVGTGDVAEAGRLLGRPYAMEGVIAKGDRMGRKIGFPTINLRSGNELY
                     PRDGVYVTEVLLRSFERTFSCVTNIGRRPTVYEDYATT"
BASE COUNT          779 a         1886 c         1917 g          779 t
ORIGIN      
        1 ccgaaggagc acggcgtcga cttcctgatg tcgcagcggc acctctggct ccgctcggcg
       61 aagcaggtgg cgatcctgcg cgtgcggagc gaggtcgaga gcgcgatcca cgatttctac
      121 cactcgcgcg gctacacgag ggtcgactcg ccgatcctga cgccgaacgc ctgcgagggg
      181 acctcgaacc tcttcgagac ccagtacacc gaggaggaga aggcgtacct ctcgcaatcg
      241 gggcagctct acctcgagcc ggccgccgcc gcgctcggga aggtctactg cttcggcccg
      301 acgttccgcg ccgagaagtc gaagacgcgc cgccacctgc gcgagttctg gatggtcgag
      361 ccggaggtcg ccttcctcga gatcgacggc ctgaaggacc tcgcggagga gttcgtcaag
      421 gagctgacgg cccgcgtcct cgaccgccgg aaggaggacc tgaagcgcct cgagagggac
      481 acgacggtcc tcgaggcgac ggtcgcgaag ccgtttcccc ggatcgacta ccgcgacgcg
      541 atcgggatcc tcgagaagaa gggcttcccc gtgaagttcg gcgacgacct cggcggcgac
      601 gaggagacgg cgctcacgga ggggttcgac acgccggtca tggtcacgcg ctacccggcg
      661 gcgatcaagt cgttctacat gcagcccgac ccgaacgacc cggaggtcgt cctcggcctc
      721 gacatgctcg cccccgaggg gtacggcgag atcatcgggg gatcccagag gatccacgac
      781 cacgacctcc tgctcgagcg aatccgccag cacgagcttc cgatcgaggc gttccagtgg
      841 tacctcgacg tgaggaagta cggctctttc ccgcactcgg gcttcggaat ggggctggag
      901 cgcttcaccg cctggatgtg cggcgtgccg cacctgcggg agtgcatccc gtacccgagg
      961 atgctctacc gcatctatcc atgatcccgg gaagcccgtt ccagcccggc tctccctgtc
     1021 ccctccgcgg cggaagagcg gccgcgcccg cggcgccgcg acggaggggt gcgtgacgga
     1081 cctcccgaac gtccgggcgg ctctcgaccg gatcgacgct gcgctcctcg acctcctcgc
     1141 cgagcgcgct gcggtcgtcg acgaggtcgc ggcgctcaag agccgggagc acgcgctcct
     1201 cctccgcgac ctcgagcggg agcgcgatct cctctcgaag gtgggtcggg acgcgcagag
     1261 gcgcggcctg aacgccttcc acgtcgtgaa gatcttccgg gacgtcgtgg aggcctccgt
     1321 gcgccagcag gagagccggc tgacgcgggc cgcgaacgcg atcgacgagg agccggcgct
     1381 cctgcgggtc gccttccagg gaacggaagg ggcctacagc cacctcgcgg ggaagaagtt
     1441 cttcgccgac cgggccgagg agccgatctt cgtcggctac cggtcgttcc gccaggcggt
     1501 ggaggcggtc gagcgggagg agtgcgagtt cgcgatcctg ccgatcgaga actcggtggc
     1561 cggagcgatc aacgagacct acaccctcct cggcacgtcg aagctgtcga tcgtcggcga
     1621 ggaagtctgg cccgtggagc actgcctcct cggcctctcg aaggtcccgc tcgcccgggt
     1681 ccggagggtc ttctcgcacc cgcaggcgct tttgcagtgc tcggagttcc tcgagagcct
     1741 cgccggggcg acggccgaga gctggttcga caccgcgggc agcgtcgcga gggtgaagga
     1801 gctcgggagc gaggagaacg ccgccatcgc ctcggaggaa gcgggggagc ggcacgggct
     1861 cgtcgtcctc aggcgttcga tcgcgaatca gaggaacaac cagacgcggt tcgtcgtcgt
     1921 ccaccggcgg ccgttccgct tcgacccgcg tatcccctgc cggacgtcgc ttctcctcgt
     1981 gacggaccat cgcgaggggg cgctcgagcg ctgcctcgcc gagctcgcga aggcgggcgt
     2041 gaacctgacg aagctcgaga gccggtcgat gccggcgact ccgtgggagt accagttcta
     2101 cgtcgacgtc gaggggaacg tggatgaccc gcgcctcgcg gaggccctcg aaggagtcgg
     2161 ccgcaacgcg cgctacctcc gggtcctcgg ctgctacccg cggaaggccg acccgtcgcg
     2221 cgaggtgccg aaggacgtcg acctcctgac ggaggccgcg ccggcggagc cctggccggc
     2281 cggcacgccg gtccccgtcg agagcccggt cgcgtatccc ctcgcggccc ggcgggcgac
     2341 cgacccgggc gcccggacgc cggtgaaggt aggggacgtc gtcttcggcg aggggttcgt
     2401 cgtgatcgcc gggccgtgcg ccgtggagaa cgaggcgcag ctcttcgagg cggcgcgcgt
     2461 cgtgcgggag gggggcggcc gcgtcctgcg gggcggcgtg ttcaagccgc ggacgagccc
     2521 ctattcgttc caggggctcg ggatcccggg cctcgacctc ctctgccgcg cggggctcga
     2581 gtaccacctg ccggtcgtta cggaggtggt cgcgcccgag gacgtcgaga aggtcgccga
     2641 gttcgccgac atgctccagg tcggggcgcg caatatgcag aacttcgccc tcctgaaggc
     2701 cgtcgggcag agcagccggc ccgtccttct caagcgcgga atgatgtcgt ccgtcgagga
     2761 gctcctcctc gccgcggagt acgtcctctc cgcggggaac cgcgccgtcg tcctttgcga
     2821 gcgcggcatc cgaacgttcg agacctcgac gcggaacacg ctcgaccttg gggcggtcca
     2881 ggtcctgaag gcgcggacgc acctgcccgt gatcgtcgac ccgtcccacg cgatgggggt
     2941 caccgcgttc gtcgcgccga tggcccgtgc ggcgctcgca gccggggccg acggggtcat
     3001 cgtcgaggtc cacccgcatc cggaggaggc gctctgcgac ggacctcagg cgctgacccc
     3061 cgagctcttc acggcgatga tgggcgacct gcggaggatg gcgcaggcgc tcggggtgaa
     3121 gatggcgtga acgggagcgc gcccgccccg gaacgagccg gatcggtctc cgacggttat
     3181 aatgaaaggt tcggacggcg gccccctggc gcggtccgtc gacacgcatg cccctgaagc
     3241 tcccctccgt cggaatcgtc tccctcggct gcccgaagaa cctcgtcgac accgaggtga
     3301 tgctcggcta cctgaagcgc gaggggctgg agctgggcga ccccgacacc tcccgggtcg
     3361 tcatcgtgaa cacctgcggc ttcatcgacc aggccaagga ggagtcggtg aacgccgtcc
     3421 tcgagcaggt ccgccgcaag gaggccgggg aggtcgaccg cgtggtcgtc gccggctgca
     3481 tggtccagaa gtacggagcc gagctcgccg cggagatccc ggaggtcgac cacttcctgg
     3541 gcctcgacga gctggagaag gcgcccgccg cggcccttgg cctcccgtcg ctcgcgcggt
     3601 tcgccgcgaa gcccctcgcg acgcgcctct acgacgacct cgccccgcgc gtcctgacgg
     3661 gtcgccgcgg ctacgcctac ctgaaggtcg ccgaggggtg caacaacccc tgcacgttct
     3721 gcacgatccc gcagatgcgc ggcctccagc gctcccggtc gatcgagtcc ctcgtccgcg
     3781 aggcccaggg gctcgaggcg cagggggtct ccgagctcgt cctcatctcg caggacacga
     3841 cccgttacgg cgaggacctc ggcctcggcc gggaggggct ctcaacgctc gtcgccgcgc
     3901 tcctccggga gacgggcttc ccgtgggtcc gattcctcta cgcctacccg aagaccctgc
     3961 acgactccgt cctcgagcgg atggcggccg agccgcggtt cgtctcgtac gtcgacgtcc
     4021 cgctccagca cgtctcgcgc tccgtcctcg cggggatgcg gcgcggcggc gacccggagt
     4081 cgtgtctccg cctcgtcgag cggctccgcg cggccgttcc cggcatcgcc ctccgctcga
     4141 cgttcatcgc cggcttcccc ggagaagggg aggccgagtt cgccgagctc ctcgcgttcg
     4201 tgaaggaggc cgggctcgac cacgtcggcg tcttccccta ctcgcccgag cccggatcgg
     4261 gggcggaagg gctcggagac ccggtcccgg ctccggtgaa gggggagcgg tgcgcggcgg
     4321 tcatggaggc gcagcgcgag atctcccgga ggaagaaccg ggagcttcgc gggaaggtgc
     4381 acgacgcgat cctggagggg gcctcgcccg agtcggagat gctcctcgag gggcgcctcg
     4441 cccggcaggc gcccgagatc gacggcaagg tcctcgtcaa cgacgttccc gagggcttcg
     4501 tcccgcggcc cggcgcgatg gtgcgggtcc gcgtgaccgg ggcgcacgac tacgacctcg
     4561 tcgggcgcgt cgtcccgtga ggcggcggcg gccgtgaacg tctatcccga cccgctccgc
     4621 aggtccgacc ttccgcgagg gggggtcgtg acgatcggca acttcgacgg cgtccacctc
     4681 ggccatcagg cgatgctcca ggccgtcgtc gcgcgcgccc gggagctcga cgcgccggcc
     4741 ctcgtcctga cgttcgaccc gcacccgacg acggtccttc atccgcagcg ggccccgaag
     4801 ctgatccaga cgctccggca gcgcgaggag gcgatcgccg ccctcggcgt cgacggcctc
     4861 gtcgtcgtcc ccttcacgcg cgacttcgcc gcgatgcccg cggaagactt cgtccgcgag
     4921 ctcctcggcc ggtccctcgg cgtgcgggaa gcccacgtcg gcgccggctt cgcgttcggc
     4981 tgcgggaagc gggggaacgt ggagctcctc tcgcgcctcg gcgaggaggc gggaatccgc
     5041 gtggcgacgc tcgacgtcgt ctcggaaggg gacgcccccg tctcctcgac gaggatccga
     5101 tcgctcgtcg ggacggggga cgtcgcggag gccgggcgcc tcctcggccg gccctacgcg
     5161 atggaagggg tcatcgcgaa gggggaccgg atggggcgga agatcggctt cccgacgatc
     5221 aatctgaggt ccgggaacga gctctacccg cgggacggcg tctacgtgac ggaggtcctt
     5281 ctccgctcct tcgagaggac gttcagctgc gtcacgaaca tcggccgccg gccgaccgtc
     5341 tacgaggact acgcgacgac g
//
LOCUS       DAOTEZ010000306         5349 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_11079_length_5349_cov_5.314885, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000306 DAOTEZ010000000
VERSION     DAOTEZ010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5349
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11079_length_5349_cov_5.314885"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="P5164_14460"
     CDS             complement(<1..584)
                     /locus_tag="P5164_14460"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY45144.1"
                     /translation="MHRRLALLAAAIPLALGGCGRPGAVERPYVISVPYDLDSLDPGH
                     RNRLSDFSLLASFYEPLVVTDANLSARPALAQRWSNPDALTWTFELRPGVRFHDGSPL
                     GAEDVVWSFERLRAEPGLELAGHVAAIASVRATGERTIEVRTKQPVAILLNKLRFILV
                     VRKGETGPTLRGRVNGTGPYRLAKLEPGREMRLER"
     gene            637..2280
                     /locus_tag="P5164_14465"
     CDS             637..2280
                     /locus_tag="P5164_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRY45145.1"
                     /translation="MSVPRTPGPRSPSGELESLRSEVEELRRLKAVDDLLETLTGVLD
                     VRQVFGRVSEISRAVLPHDALALGRITSDRQKIRVWAHASVPEGFDVPETVPLPDPTV
                     VQREWDFMVLDDITADETLRRQNAPLLKFGIRSVLRVPLKEEGVIVAALNFLSFRAGA
                     FAARDVLVAKRISAFVHLALSHHDLAEAARHAAEAQERARRLERRVEGLVAALAAHGR
                     ASRVVGVSAPWKEVLRQAAKVSSAETTVLLTGESGTGKEVVARFLHASSPRATGPFVA
                     INCAALPDTLLESELFGFERGAFSGAHAAKPGRIEAAQGGVLFLDEIGETSPSVQAKL
                     LRVLQEKEFQRLGGNRTQRADVRVVAATNRDLASAMARGTFREDLFYRLAVFEIRLPP
                     LRERPDDILPLAGAFLEDIGAELGRPAAGISRDAMDALLRYRWPGNVRELRNVLERAS
                     ILSDGGLITHEHLIVPQPERPHAGTAALAAPSAPERPGLPSPSRTFRAESLAELERRA
                     IEKALAEARFNKSRAARALGLTRGQLYGKLRRLGIDPVD"
     gene            2452..2703
                     /locus_tag="P5164_14470"
     CDS             2452..2703
                     /locus_tag="P5164_14470"
                     /inference="COORDINATES: protein motif:HMM:NF038399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Os1348 family NHLP clan protein"
                     /protein_id="HRY45146.1"
                     /translation="MSQKTVEHIIGRLATDEEIRLRFRTDPVATITAAAGEPEALTNV
                     EREALASIDPDVLDRLADAVDPRLQRVRIPAERGGGGRR"
     gene            2700..4298
                     /locus_tag="P5164_14475"
     CDS             2700..4298
                     /locus_tag="P5164_14475"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRY45147.1"
                     /translation="MKASTLLLVLAALRAGVLNADDPAPELRLTLAEARETALERNVE
                     LRVEREAAAIADAGELRARGAWDPLLRVDVRARERTDPVNSILSGAPAGELAPTVRSA
                     GLSLGVVQLLPTGGSLTLTSTLAREKTDNVFSILSPAWTSSLGLEIRQPLLRNRVIDP
                     ARAALRVARIDRGRSEASLRRIASDTVAAVERAYWSLVAARQAVEAQASAVALAERQQ
                     EDVKARVEAGTLSESDLATPLAELERRKGELLASREAAARAENALKALLLGDPADPAW
                     ASRLVPADEAEPAPAPSAAPDVAAAEERRPEVAEAAARLDRLAIEDEAARDHARPQLD
                     LVAGYTGRGLAGDLNPGVAPPFPSGPVEVPAALDGGPGRSLGTLGEGRYPDASVGLSF
                     ALPLGNRTARAESKQLRSARRQAELALLRARQQVGVEVRNAALAEETAEQRVAAARAA
                     HRAAETQLRAEEERFAAGLTTSFFVLTRQNDLTQALLVETRALADLRTARVERARAEG
                     TLLSDRQIQLHDAAPAAVPERGTR"
     gene            4295..5329
                     /locus_tag="P5164_14480"
     CDS             4295..5329
                     /locus_tag="P5164_14480"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY45148.1"
                     /translation="MNRASRRLAAAVLLPLLLAGCQKAGESGATRLNGRIEAAMVDLA
                     PKVAGRVLEVKVREGDRVKAGDLLVTLDLGETALSVERDRDALGSASARLRDLQSGSR
                     SQEVAAAEADLADKTAAVELARKELARQEALMARRVGIPRDLDRARTELERTLAVRSA
                     SAERLALAREGFRKFQTEQARADVRRAETILKQSETIVREAEIRAPADGVVLHRMAEP
                     GLLLATGQPALTLAFADRLYVRTFVPETRLGRVRPGLSAQVFVDAFPGRAFPARVTEI
                     SPTAEFTPKAVETREERVNLVYAAKVDLDAGWDAALVPGQPAEVVVETGGEAPAGTTK
                     PPAVPSASLR"
BASE COUNT          732 a         2066 c         1855 g          696 t
ORIGIN      
        1 cgttcgagcc gcatctcccg cccgggctcg agcttcgcga gccggtacgg gccggtcccg
       61 ttcacgcgcc cgcggagcgt cggtccggtc tcgcccttgc ggacgacgag gatgaacctc
      121 agcttgttca gcaggatcgc gaccggctgc ttcgtccgca cctcgatcgt ccgctctccc
      181 gtcgcgcgga cgctcgcgat cgccgcgacg tgtccggcca gctcgagccc cggctccgcg
      241 cggaggcgtt cgaacgacca gacgacgtct tccgctccca gggggctccc gtcgtggaac
      301 cggacgcccg gccgcagctc gaaggtccag gtgagcgcgt ccgggttgct ccagcgctgc
      361 gcgagcgcag ggcgcgccga gaggttcgcg tcggtcacga cgagcggctc gtagaagctc
      421 gccaggagcg agaagtcgct gagccggttc ctgtgccccg gatcgagcga atcgaggtcg
      481 taggggacgg agatcacgta gggtcgctcg acggccccgg gccggccgca acccccgagg
      541 gcgagcggga tcgcggcggc gaggagggcg agccggcggt gcacgttcgc gcgagtatag
      601 gagcccgacc tcgcgtggct ccgtagaatc ggcctgatga gcgtgccgcg aacgcccgga
      661 cctcgttcgc cgtccgggga gctcgagagc ctccggtccg aggtggagga gctgcggcgg
      721 ctcaaggccg tcgacgacct cctcgagacg ctcacgggcg tcctcgacgt ccggcaggtc
      781 ttcggccgag tgtcggagat ctctcgcgcg gtcctgcctc acgacgcgct cgccctcgga
      841 cgcatcacgt ccgaccggca aaagatccgg gtctgggctc acgcctccgt cccggaaggc
      901 ttcgacgtcc ccgagacggt ccccctcccc gaccccaccg tcgtccagcg ggaatgggac
      961 ttcatggtcc tcgacgacat cacggccgac gagacgctcc ggcgacagaa cgcccccctc
     1021 ctgaagttcg gaatccgcag cgtcctgcgc gtcccgctga aggaggaggg ggtgatcgtc
     1081 gcggcgctca acttcctgtc gtttcgggcc ggcgccttcg ccgcgcgcga cgtcctcgtc
     1141 gcgaagcgga tctccgcgtt cgtgcacctc gccctctcgc atcacgacct cgcggaggcg
     1201 gcgcgccacg ccgccgaggc tcaggagcgc gcccggcgcc tggaacgccg agtcgaaggg
     1261 ctcgtcgccg cgctcgcggc ccacggccgg gcctcgcgcg tcgtcggcgt ctccgctccc
     1321 tggaaggagg tccttcgcca ggcggcgaag gtctcgtcgg ccgagacgac cgtcctcctc
     1381 acgggggagt cggggaccgg gaaggaggtc gtcgcacggt tcctccacgc ctcctcgccg
     1441 cgcgcgacgg gtccgttcgt cgcgatcaac tgcgccgccc tccccgacac gctcctcgag
     1501 tccgagctct tcggcttcga gcgaggcgcg ttctccgggg cgcacgcggc gaagccgggc
     1561 cggatcgagg ccgcgcaggg gggcgttctc ttcctcgacg agatcggcga gacgagcccc
     1621 tcggtccagg ccaagctcct gcgcgtcctc caggagaagg agttccagcg gctcggcggg
     1681 aaccggacgc agcgggccga cgtccgcgtc gtcgccgcca caaaccgcga cctcgcgtcc
     1741 gccatggcgc gtggcacgtt ccgcgaggac ctcttctacc ggctcgccgt cttcgagatc
     1801 cgtctcccac cgctccggga gcggcccgac gacatcctcc cgctggccgg cgccttcctc
     1861 gaggacatcg gtgccgagct cggccgtccg gcggccggga tctcccggga cgccatggac
     1921 gcgctcctgc gctaccggtg gcccgggaac gtccgcgagc tccggaacgt cctcgagcgc
     1981 gcctcgatcc tcagcgacgg gggcctcatt acgcacgagc acctcatcgt gccgcagccg
     2041 gaacgcccgc acgccggcac ggcggcgctc gccgctccgt cggccccgga acgtcccgga
     2101 ctcccatcgc cctcccgcac gttccgtgcg gaatctctgg ccgagctcga gcgcagggcg
     2161 atcgagaagg cgctcgcgga ggcacgcttc aacaagagcc gcgccgcacg agccctcggc
     2221 ctcacgcgcg gtcagctcta cgggaagctc cgccgactcg gcatcgaccc cgtcgattga
     2281 tccgatcgga gcaacatgct ccgatcggat caactcgatt cgacgacgtc acgccgacct
     2341 ccggccgtcc gggaacctca acacgttccc tggcaacgaa atgcgccatc accacccgag
     2401 tcgtcgacga tggcagaacg cttgcgaccg actcggtgaa ggatgctcga gatgagccag
     2461 aagaccgtcg aacacatcat cggccggctc gccacggacg aggagatccg gctgcgcttc
     2521 cggacggacc ccgtcgcgac gatcacggcg gccgcgggcg agcccgaagc gctcacgaac
     2581 gtcgagaggg aggccctcgc gtcgatcgac cccgacgtcc tggaccggct cgccgacgcc
     2641 gtcgaccctc gcctgcagcg agtgaggatt cccgcggagc gcggcggagg gggaagacga
     2701 tgaaagcgtc taccctgctc ctcgtcctcg cggccctccg ggccggcgtg ctgaatgccg
     2761 atgaccccgc tccggagctg cgcctcacgc tcgccgaggc ccgcgaaacc gcgctcgagc
     2821 ggaacgtcga gctccgcgtc gagcgggaag cggccgccat cgccgacgcg ggcgagctcc
     2881 gcgcgcgggg agcctgggac ccgctcctcc gggtcgacgt ccgggcgcgg gaacggaccg
     2941 acccggtcaa ctcgatcctc tccggggcgc cggccggcga gctcgcgccg accgtccgga
     3001 gcgcgggcct ctccctcggc gtcgtacagc ttctcccgac cggcggctcg ctcacgctca
     3061 cctcgacgct cgcgcgagag aagaccgaca acgtcttctc gatcctctcc cccgcctgga
     3121 cgagctcgct cgggctggag atccggcagc cgctcctgcg aaaccgcgtc atcgatcccg
     3181 cccgcgcagc gctccgcgtc gcgcggatcg accggggccg atcggaggcc tcccttcgcc
     3241 gcatcgcctc cgacaccgtc gccgcggtcg aacgcgccta ctggtcgctc gtcgcggccc
     3301 gccaggcggt ggaggcccag gcgtctgccg tcgctctcgc cgagaggcag caggaggacg
     3361 tgaaggcccg cgtcgaggcg gggacgctct ccgaatcgga cctcgccacg ccgctcgccg
     3421 agctggagcg gcggaagggg gagctgctcg cctctcgcga ggccgccgcg cgcgccgaga
     3481 acgccctgaa ggcgctcctt ctcggcgacc ccgccgatcc ggcctgggcg agccgcctcg
     3541 tccccgccga cgaggccgag ccggcgccag ccccctccgc ggcgcccgac gtcgccgccg
     3601 ccgaggagcg gcgtcccgag gtggccgagg cggccgcccg cctcgaccgg ctggcgatcg
     3661 aggacgaggc tgcccgcgac cacgctcgtc cgcagctcga cctcgtcgcc ggctataccg
     3721 gccgcgggct agccggagac ctcaaccccg gggtcgctcc ccccttcccg agcggtcccg
     3781 tcgaggtccc cgcggcgctc gacggcgggc cgggccgatc gctcggcacg ctcggcgagg
     3841 ggcgctaccc cgacgcctcg gtcggcctct ctttcgcgct gcccctcggc aaccgaacgg
     3901 cgcgggccga gtcgaagcag cttcgctccg cgcgccgcca ggccgagctc gccctcctcc
     3961 gggcgcgtca gcaggtcggc gtggaggtga ggaacgcggc cctggccgag gagacggccg
     4021 agcagcgcgt ggcggccgcc cgcgccgcgc accgggccgc cgagacgcag ctgcgcgccg
     4081 aggaggagcg cttcgccgcg ggcctgacga cgagcttctt cgtcctgacc cgccagaacg
     4141 acctgacgca ggcgctcctc gtcgagacgc gcgccctcgc cgacctccgg acggcgcgcg
     4201 tcgagcgcgc ccgggcggag gggacgctcc tctccgaccg tcagattcag ctccacgacg
     4261 ccgcgccggc cgccgtgccg gagagaggaa cccgatgaac cgcgcttccc gccgcctcgc
     4321 cgcggccgtg ctccttcccc ttctcctcgc cggctgccag aaggccggcg agagcggcgc
     4381 gacccgcctc aacgggagga tcgaggccgc catggtcgat ctcgccccga aggtcgcggg
     4441 ccgggtcctc gaggtgaagg tccgcgaggg cgaccgcgtg aaggcgggcg acctcctcgt
     4501 caccctggac ctcggagaga cggccctctc tgtcgaaagg gaccgcgacg ccctcggctc
     4561 ggcatccgcc cgccttcggg acctccagtc gggaagccgg agccaggagg tcgcggccgc
     4621 cgaggcggac ctcgccgaca agacggcggc cgtggagctc gcccggaagg agctcgcccg
     4681 ccaggaagcc ctcatggcga ggagggtcgg gataccgagg gacctcgacc gcgcccggac
     4741 ggagctcgag aggaccctgg ccgtccggtc ggcgagcgcg gaacgcctcg ccctcgcccg
     4801 cgaaggattc cggaagttcc agaccgagca ggcgcgggcc gacgtccgtc gtgccgagac
     4861 gatcctgaag cagtcggaga cgatcgtccg cgaagccgag atccgcgcgc cggccgacgg
     4921 cgtcgtcctc caccggatgg ccgagccggg tctcctcctc gccacgggtc agcccgcgct
     4981 gaccctcgcg ttcgcagatc ggctctacgt ccgcacgttc gtccccgaga cccggctcgg
     5041 tcgggtccgg cccggtcttt ccgcccaggt cttcgtcgac gccttccccg ggcgggcctt
     5101 ccccgctcgc gtcaccgaga tctctccgac cgcggagttc acgccgaagg cggtcgagac
     5161 gcgcgaggag cgggtcaacc tcgtgtacgc cgcgaaggtc gatctcgacg ccggctggga
     5221 cgcggccctc gtcccgggcc agcccgcgga ggtggtcgtc gagaccggcg gggaggcgcc
     5281 cgccggcacg acgaagccgc cggcggtccc gtcggcttcc cttcgctgag acggcgatga
     5341 tcgaagccc
//
LOCUS       DAOTEZ010000307         5346 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_11090_length_5346_cov_7.266490, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000307 DAOTEZ010000000
VERSION     DAOTEZ010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11090_length_5346_cov_7.266490"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="P5164_14485"
     CDS             <1..483
                     /locus_tag="P5164_14485"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HRY45149.1"
                     /translation="VASTISTISLAIFGGLGNAEAEARGRDLATALQLTNVVRDVGED
                     VGRGRLYLPLEDLARFGVTEADLVSLRATPAAAELVRFEARRALSYFRRAEPVKELVD
                     RECRFAVTMMGGIYADVARAVLARPLDTLRRRISLSTPGKVRAVLARLADRRFDRGED
                     "
     gene            517..897
                     /locus_tag="P5164_14490"
     CDS             517..897
                     /locus_tag="P5164_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45150.1"
                     /translation="MALDEKTRAALRQLGKALNEAVSSSAQVHGILQSLREEGHEPYL
                     VLDATVALDARGRRARAALPSIRRGGPANREEAEGAVFKIDVKDLHFLRSLGIDPTRA
                     PRAPRGRRRTGTAVSLRTTVSKRV"
     gene            952..2040
                     /gene="rlmN"
                     /locus_tag="P5164_14495"
     CDS             952..2040
                     /gene="rlmN"
                     /locus_tag="P5164_14495"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRY45151.1"
                     /translation="MTTPLLGLSLPELAQALAPFARERFRAAQVQRWMTERRARSFDE
                     MTDLPAPLRAALGERFVVGLPEVEARTPSEDGAEKFLFRLADGAKVEAVFIPAPRRRA
                     SGTGERVTVCVSSQAGCALACAFCVTGRMGAGRNLTAGEILGQYLVVAREKGFGPRAA
                     NVVFMGMGEPLLNVANVSRALDLLAAEVSPRRTTVSTAGIVPGIRELAKREHRPNLAV
                     SLTAAEDALRTRLMPINAQWSVRELFEALEEWPLETGRRVTLEVVLIADVNDSPEAAR
                     ALSRLARRVPSKVNLIPLNESPEWLPGLARPADEKVNAFGALLAASGVDVTVRWSKGL
                     GAAAACGQLKGRETPGRVLRVPAPSVRS"
     gene            2066..2857
                     /locus_tag="P5164_14500"
     CDS             2066..2857
                     /locus_tag="P5164_14500"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY45152.1"
                     /translation="MARTFLALTLAAPLLLVAPPAGAQTAPAPTAAPAPPTPAEAARG
                     AERKLDSWMTADARTALTPVVAQAGANVDVAVALGRLLEQEKKYAESAEVLQKAVALA
                     PADPRPSHWLGETLLRARKTAEADAAFRKAAELATKAAEARPADAGAFLAKGAALSRL
                     RRYDEAMTALAKARELDGGSAATLYQMGSTRAFQQKWSDAVDLLTQTLAKDPGVALAY
                     YYRGLAQEKLGRKDQMVLDLDRFVKIAATAPEAERAKALLAAASR"
     gene            complement(2939..3544)
                     /locus_tag="P5164_14505"
     CDS             complement(2939..3544)
                     /locus_tag="P5164_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRY45153.1"
                     /translation="MKKNLLSAALSAVLVVAGAPALAETWTADTAHSAVGFSVRHMMV
                     SNVKGAFNKFTATVEGSPADPASAKINATIEVASVDTREPKRDEHLRSADFFDAAKHP
                     QMTFVSTKVEKVSATKAKVTGNLTLRGVTKPVTLDVEYTAPVKSPWGQTVIGATATGK
                     IDRQDFGVSFSKALETGGVLVGDDVTLLLELELVKQDAPKK"
     gene            complement(3721..>5346)
                     /locus_tag="P5164_14510"
     CDS             complement(3721..>5346)
                     /locus_tag="P5164_14510"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRY45154.1"
                     /translation="FATPLERTELAAGLPSADARLLEGLRALGYPYAVLGGRELLDDG
                     RLVVRVERGEPSLVDSVEIRDVSPEERERFGQLVRLAPGDVADSERTALSALAIEEAL
                     RAEGFATARVRATLSPATPEIPPRLALTLAVERGPAQRIGSVRLEGLARTSTAWAKSV
                     VALSPGDVFRRSDLDAARAGLFSLGPFRSVRGDLTTGPDGRVDVVLSAEELPPVTLAY
                     GVRWENERGVSAVVDASDRNLFGRGIVAGVRGLYDPEDRALRLFAGLPEQVLGAGIDL
                     WVERRRSFREGLFYGQRTDAVEASLQLSRSFGKSLSARLYGRFKETRYLEDDIFFPID
                     VTIRLPYLGVQLVHDTREDPMLGTNGVLATLDLQSSGSWLGSSFAFGRAYGQLNFYRP
                     VFALGSGRVVWAQSVRAGFARAFEGQELIPDVRFYAGGSYSVRGYGTESLGPREDLGG
                     TLYVTGGSTLLVVNEEIRVPLHPRLLGVGFFDAGQVWESSRDFGTGLATSVGLGLRAL
                     TPLGVLRLDGAVPLKRREGDPAWRITFGFGNVF"
BASE COUNT          720 a         1959 c         1968 g          699 t
ORIGIN      
        1 gtggcctcga cgatctcgac gatctccctc gcgatcttcg gcggtctcgg gaacgcggag
       61 gccgaggcgc gcgggcgcga cctcgcgacg gcgctccagc tgacgaacgt cgtgagggac
      121 gtcggcgagg acgtgggccg cggccggctc taccttcctc tcgaggacct cgcgcgcttc
      181 ggcgtgacgg aggcggacct cgtgtcgctc cgcgccacgc ccgccgccgc cgagctcgtc
      241 cgcttcgagg cgcgccgcgc gctctcctac ttccggaggg ccgagccggt gaaggagctc
      301 gtcgaccgcg agtgccggtt cgcggtgacg atgatgggcg gcatctacgc cgacgtggcg
      361 cgggcggtcc tcgcgcgtcc gctcgacacg ctgcgccgcc gcatctcgct ctcgacgccg
      421 ggcaaggtgc gcgccgtgct cgcgcggctc gccgaccgcc gcttcgaccg cggcgaagat
      481 tgacgcgcgc cgctcggccg ctctagagtc gagccgatgg ctctcgacga gaagacccgc
      541 gcggcgctcc gccagctggg gaaggccctg aacgaggccg tctcctcctc ggcccaggtg
      601 cacggcatcc tccagagcct ccgcgaggag gggcacgagc cgtacctcgt cctcgacgcg
      661 accgtcgccc tcgacgcccg cggacgccgc gcccgcgcgg cgctcccgtc gatccgccgc
      721 ggaggccccg cgaatcgcga ggaggcggag ggggcggtct tcaagatcga cgtgaaggac
      781 ctccatttcc tgcgctccct cgggatcgac ccgacgcgcg ccccccgtgc gccgcgcgga
      841 cggcggcgga ccgggacggc cgtctcgctc cggacgaccg tctcgaagcg ggtctgaggg
      901 cgtgcgcgcg ccgcgacgcg ggagagcgcg ccgggccccc gcggatccga cgtgacgacc
      961 ccgctcctcg ggctctcgct ccccgagctc gcacaggcgc tcgctccgtt cgcgcgagaa
     1021 cgctttcgcg cggcgcaggt gcagcgctgg atgacggagc ggcgcgcccg ctcgttcgac
     1081 gagatgaccg acctccccgc cccgctgcgc gcggcgctcg gggagcggtt cgtcgtcggg
     1141 cttccggagg tcgaggcgcg gacgccgtcc gaggacggcg cggagaaatt cctcttccgc
     1201 ctcgccgacg gagcgaaggt cgaggccgtc ttcatccccg ccccgcggcg gcgcgcctcg
     1261 ggcacgggcg agcgcgtcac cgtctgcgtc tcctcgcagg cgggctgcgc gctggcctgc
     1321 gccttctgcg tgacggggcg gatgggagcc ggacgaaacc tcacggccgg cgagatcctc
     1381 gggcagtacc tcgtcgtcgc ccgcgaaaag ggcttcggcc cgcgcgcggc gaacgtcgtc
     1441 ttcatgggga tgggcgagcc gctcctgaac gtcgcgaacg tctcgcgggc gctcgacctc
     1501 ctcgcggcgg aggtctcgcc gcgccggacg accgtctcga cggcggggat cgtcccgggg
     1561 atccgggagc tggcgaagcg ggagcatcgc ccgaacctgg ccgtctccct caccgcggcg
     1621 gaggacgcgc ttcgcacccg cctcatgccg atcaacgcgc agtggtccgt ccgggagctc
     1681 ttcgaggcgc tcgaggagtg gccgctcgag acgggccggc gcgtcacgct cgaggtcgtc
     1741 ctgatcgccg atgtgaacga ctcccccgag gcggcccgcg ccctctcgcg cctcgcccga
     1801 agggttccgt ccaaggtgaa cctcatcccg ctcaacgaat cgcccgaatg gctccccggc
     1861 ctcgcacgcc ccgccgacga gaaggtgaac gccttcggcg cgctcctcgc ggcctcgggc
     1921 gtcgacgtga cggtccgctg gtcgaagggg ctcggcgccg cggccgcttg cggccagctg
     1981 aaggggcgcg agacgcccgg ccgggtgctg cgcgtcccgg cgccgtccgt gcgatcctga
     2041 cggcagttcc gaaccaggag gctccatggc acgaacgttc ctcgccctga ccctcgccgc
     2101 cccgctcctc ctcgtcgcgc cgcccgccgg cgcgcagaca gccccggcgc cgaccgcggc
     2161 tccggcgccg ccgactccgg cggaggccgc gcgcggcgcc gagcggaagc tcgactcctg
     2221 gatgacggcc gacgcgcgga ccgccctcac tccggtcgtc gcccaggccg gcgcgaacgt
     2281 cgacgtggcg gtcgcgctcg gccggctcct cgagcaggag aagaagtacg ccgagtctgc
     2341 ggaggtcctc cagaaggccg tcgcgctcgc ccccgccgat ccccgcccct cccactggct
     2401 cggcgagacg ctcctgcgcg cccggaagac ggccgaggcc gacgccgcgt tccggaaagc
     2461 ggcggagctg gcgacgaagg cggcggaggc gcggccggcc gacgccgggg ccttcctcgc
     2521 gaagggagct gcgctctccc gcctgcgccg ctacgacgag gcgatgacgg cgctcgcgaa
     2581 ggcgcgcgag ctggacggcg ggagcgccgc gacgctctac cagatgggct cgacccgcgc
     2641 gttccagcag aagtggagcg acgcggtgga cctgctcacg cagacgctcg cgaaggaccc
     2701 gggagtggcc ctcgcgtact actaccgcgg cctcgcccag gagaagctcg ggcggaagga
     2761 ccagatggtc ctcgacctcg accgcttcgt gaagatcgcc gcgacggccc cggaggcgga
     2821 gcgcgcgaag gccctcctcg ccgccgcgtc gcgctgacgg gcgctcccgg aacgcgaagg
     2881 agcccgcggt ccctctcggg gaccgcgggc tccgccgctc ggcgcgctcg ccgcgcgctt
     2941 acttcttcgg cgcgtcctgc ttcacgagct cgagctcgag gaggagcgtc acgtcgtcgc
     3001 cgacgaggac gccgcccgtc tcgagcgcct tcgagaagct gacgccgaag tcctgccggt
     3061 cgatcttccc cgtggccgtc gcgccgatga cggtctggcc ccacgggctc ttcaccgggg
     3121 cggtgtactc gacgtcgagc gtgaccggct tcgtcacgcc gcggagcgtg aggttgccgg
     3181 tgaccttcgc cttcgtggcg gagaccttct cgaccttcgt ggagacgaac gtcatctgcg
     3241 ggtgcttcgc cgcgtcgaag aagtcggccg agcggaggtg ctcgtcgcgc ttcggctcgc
     3301 gggtgtcgac ggaggcgacc tcgatcgtcg cgttgatctt cgcggaggcc gggtcggcgg
     3361 ggctcccctc gaccgtcgcg gtgaacttgt tgaacgcccc cttcacgttc gagaccatca
     3421 tgtggcgaac ggagaagccg acggccgagt gggcggtgtc ggcggtccag gtctcggcga
     3481 gcgcgggggc tccggcgacg acgaggacgg cggagagggc ggcggagagc aggttcttct
     3541 tcatcggttc ctcctgggtt ctttctcggg ggcgtgcccc gttctcggat ctgttcgggc
     3601 gccgcgggat ctcgcgcggc atctcgctca tcgcaactcg tgtgccagcc gccaggccgc
     3661 gcaaactgcg atttcagcga tcggatcggg acaggcgcct ctcgaaacga gaggttccgc
     3721 tcagaagacg ttcccgaaac cgaacgtgat gcgccaggcg gggtccccct cgcgccgctt
     3781 cagcgggacg gccccgtcga ggcgcaggac gccgagcggc gtgagcgccc gcaggccgag
     3841 cccgacggag gtcgcgagac cggtcccgaa gtcgcgggac gactcccaga cctgtcccgc
     3901 gtcgaagaag ccgacgccga ggaggcgcgg gtggagcggg acccggatct cctcgttcac
     3961 gacgaggagc gtcgagccgc cggtgacgta gagcgtgccg ccgaggtcct cgcgcgggcc
     4021 gaggctctcc gtcccgtagc cgcgcaccga gtaggacccg ccagcataga agcggacgtc
     4081 ggggatcagc tcctgcccct cgaacgcgcg tgcgaagcct gcgcgcaccg actgcgccca
     4141 gacgaccctc ccgctcccga gcgcgaagac cgggcggtag aagttcagct ggccgtaggc
     4201 acggccgaac gcgaagctgg acccgagcca gctcccgctc gactgcaggt cgagcgtcgc
     4261 gaggacgccg ttcgtcccga gcatcgggtc ctcgcgcgtg tcgtggacga gctggacgcc
     4321 gaggtagggg aggcggatcg tcacgtcgat cgggaagaag atgtcgtcct cgaggtagcg
     4381 cgtctccttg aagcgtccgt agagacgcgc cgagaggctc ttcccgaagc tgcgcgagag
     4441 ctggagcgag gcctcgaccg cgtcggtcct ctgaccgtag aaaaggccct cgcggaacga
     4501 acgccgccgc tcgacccaga ggtcgattcc cgctccgagg acctgttccg gcaggccggc
     4561 gaagaggcgc agggcacggt cctcggggtc gtagaggccg cggacgccgg cgacgatccc
     4621 gcgaccgaag aggttccggt ccgaggcgtc gacgacggcc gagacgcccc gctcgttctc
     4681 ccagcggacg ccgtaggcga gcgtcaccgg ggggagctcc tcggccgaga ggacgacgtc
     4741 gacgcggccg tcgggtcccg tcgtgaggtc gcctcggacg ctccggaacg gaccgagcga
     4801 gaagaggccg gcgcgcgccg cgtcgaggtc ggaacggcgg aagacgtccc cgggggaaag
     4861 tgccacgacg ctcttcgccc acgccgtcga ggtccgtgcg agaccctcga gcctcacgct
     4921 cccgatccgc tgcgcggggc cgcgctcgac ggcgagggtg agggcgaggc gcggagggat
     4981 ctccggcgtc gcgggggaga gcgtcgcgcg gacccgcgcg gtcgcgaagc cctcggcccg
     5041 cagggcctcc tcgatcgcga gcgccgagag cgcggtccgc tccgagtcgg cgacgtcgcc
     5101 cggcgcgaga cggacgagct gcccgaagcg ctcgcgctcc tccggcgaga cgtcccggat
     5161 ctcgacggag tcgacgaggg acggctcgcc tctctccacg cggacgacga gccgcccgtc
     5221 gtcgaggagc tcgcgccccc cgaggacggc gtacgggtag ccgagggcgc ggagcccctc
     5281 gaggagcctc gcgtcggcgc tcggcaggcc ggcggcgagc tcggtccgct cgagcggggt
     5341 cgcgaa
//
LOCUS       DAOTEZ010000308         5345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_11096_length_5345_cov_3.465028, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000308 DAOTEZ010000000
VERSION     DAOTEZ010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11096_length_5345_cov_3.465028"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="P5164_14515"
     CDS             complement(<1..652)
                     /locus_tag="P5164_14515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRY45155.1"
                     /translation="MSKEPAELVPGEEVLPLAADWALAAFENAPIGMVVTSLRGRILR
                     YNRAFAALLGYTPEELGRVSWASVTHPDDVEASHALVARLLAGEASSGRIAKRYLGKG
                     GRVAHAEVSTSLLRDERDRPLYLVTHVVDVTRRREEERERERAAAEILDLYDNAPCGY
                     HSLDGDGIFLRVNATELAWLGYRREELVGRRRFTDLLTPAGLETFRTSFAELKERGF"
     gene            complement(783..1124)
                     /locus_tag="P5164_14520"
     CDS             complement(783..1124)
                     /locus_tag="P5164_14520"
                     /inference="COORDINATES: protein motif:HMM:NF025601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HRY45156.1"
                     /translation="MKGPKVRVEELEVTGEKLLATVRELVRQGNVRRIVIRDAKGVTL
                     LEIPLVVGLAGAVLVPIWAAIGALAALLAKMTLVVERVQDAPAAPPPPEAAERPKPRR
                     RASGRRPVDDD"
     gene            1258..5007
                     /locus_tag="P5164_14525"
     CDS             1258..5007
                     /locus_tag="P5164_14525"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRY45157.1"
                     /translation="MARTPREKDPAKDAGNLGDAGAFRRFWPYAALLVLLGAGLVVVH
                     VGFAKAARRDCQNLLTTLADQQKAAIEAHLDQRIADARVFAAFPSVRALLASGDGLGA
                     EDSAHLGEVLEAGRAEWGARAIQVLGEDGTVRAGSGELLPSGAAALVAGAGEKPVVVG
                     LVGDGEVSRLVVAARVRAPDGALRGWAVFVDDPAGSLWKILRREPVLSRTGEALLVST
                     EEGAPVLLSPARHIVSDQSRSRERRLGPGRGALETLGGSGRFGELVDYRGETVWAAFR
                     RIDRTRWGLVVKIDRAEALQSRASGRLWQSLALVFLALAAAATFRSIRTSERLRTAAE
                     LQRRDERHRLVLEQVRDAVVWHRPGDGRILEANGAATELWGLTREGLLAGSLFDLLAA
                     EDVTEARLKMSGARRVGGLIRTRGKRRDGSLFPADISARAVVLEGEEILVSVVRDVSA
                     AEAALGQIRFLNRVLRTLTAVDQVLVEVHDRETVLRRTCEEIVSTGGFLAAWFGVPDA
                     EGRVVPAASAGALAGYLEEVKITTDAGPLGRGPVGTAFREGRTVVVDDWASDPAVEPW
                     REAATRRGFRSGASCAVRTGEKTIGVLTLYAERAGAFPQEAVLLLEELARDLGLALEL
                     IEAGERRRRAEESLAASEARYRMLFEDNPGPMWVFDVESLRFLAVNEAAVSLYGYSRE
                     EFLEMSLREIRPAEDEAALRADVQVPRSGVRSSGPWRHRTKDGRILLVEIVTHDVTYG
                     GRPARLVLVNDVTERVRAEEKLRAFFDSGMLGAFLSDADGRLTAANDEFLRIVGRTPA
                     EVEEGALTWRGLTPPEWIEVDVARAAEARGRGVCTPYEKEYLRKDGSRVPVLVGFALV
                     GEGRDESVAFVLDRTEGKRAGEELRRSEERFTKFLDSLPAAAFVKAADGRVLWANRFL
                     EALLGAGPLAGRTTYEVLPAGAAAVMTEDDRRAAAGETVERMEHLPDADGRTRVYRTL
                     KFPVPGRDGEPWSGGISIDVTAQVRAEEEVRLLNAELEARVERRTEELVAKSRELESF
                     AYSVSHDLRAPLRAIDGFSRMVEEDHATGLDAEGRRLLGVVRDAARRMGRLIDDLLAF
                     SRAGRQELKRTHVDVAELVRSVVPEVLPEAEREKSDVSIGDLPPVAADASLLRQVFVN
                     LLSNAVKFSATRTRRRIAISGRREAGRVDYDVEDNGVGFDMEYAGKLFGVFQRLHGRE
                     FDGTGVGLALAERIVSRHGGSISAWAEPDRGARFTVSIPETGGEE"
     gene            5010..>5345
                     /locus_tag="P5164_14530"
     CDS             5010..>5345
                     /locus_tag="P5164_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY45158.1"
                     /translation="MQEAPDVLVVEDNPDDVELTLRALRRMHLTNPVRVARDGVEALE
                     VLLGDGTTRPPLPRVVLLDLKLPRVGGLEVLARLRQEERTRTLPVVVLTSSREEPDVK
                     QAYALGVNSY"
BASE COUNT          706 a         1863 c         2032 g          744 t
ORIGIN      
        1 cgaagccccg ctccttgagc tcggcgaagc tcgtgcggaa cgtctcgagg cccgccgggg
       61 tcaggagatc ggtgaacctc ctgcgtccga cgagctcctc ccgacggtag ccgagccagg
      121 cgagctccgt ggcgttgacc cggaggaaga tgccgtctcc gtcgaggctg tggtagccgc
      181 agggagcgtt gtcgtaaagg tccaggatct ccgcggccgc cctctcccgc tccctctcct
      241 cctcgcgccg gcgcgtcacg tccacgacgt gcgtcacgag gtagaggggg cggtcccgct
      301 cgtcgcggag gaggctcgtc gagacctcgg cgtgggcgac ccgccccccc tttccgaggt
      361 atctcttcgc gatccgcccc gaggaggcct cgccggcgag gagcctcgcc acgagcgcgt
      421 gcgaagcctc gacgtcgtcg gggtgcgtca ccgaggccca gctcacccta ccgagctcct
      481 cgggcgtgta gccgagaagg gcggcgaacg cacggttgta ccggaggatt cggccgcgga
      541 gcgaggtgac caccatgccg atcggggcgt tctcgaacgc cgcgagcgcc cagtcggccg
      601 cgaggggaag aacctcctcc ccggggacca gctcggccgg ctccttggac atcgcgcgac
      661 gccctcccgt cggccccatt ctggcaccgg ttcgtgcccg tccggccgga cgcggcatcg
      721 gggaaacaga ggagcgcgcg cgcccggggc cgagaccggt cccgcggcga tcgcggcctg
      781 gctcagtcgt cgtcgacggg ccggcgcccg ctcgcgcggc ggcgcggctt cgggcgttcg
      841 gcggcctcgg gtgggggcgg cgctgcggga gcgtcctgga cccgctcgac gacgagcgtc
      901 atcttcgcga ggagcgccgc gagggcaccg atcgccgccc agatcgggac gagaacggcc
      961 cctgcgagcc cgacgacgag cgggatctcg aggagcgtca cccccttcgc gtcgcggatg
     1021 acgatgcggc ggacgttccc ctggcggacg agctcccgaa ccgtcgcgag cagcttctcg
     1081 ccggtcacct cgagctcctc gacgcgaact ttcggaccct tcatggcgcg cctccgcgac
     1141 gaggtacggc gggaatggcg ggagggttcg cgccggagac gcccgggcgg gctcgaaccc
     1201 gctcgggaag gtccgggatc gcggtactct cgtctcgagg agcaggtggg ctcggccgtg
     1261 gctcgaaccc cgagagagaa ggacccggcg aaagacgccg ggaatctcgg cgacgccggc
     1321 gcgttccgcc gcttctggcc gtacgccgcc ttgctcgtcc tcctcggtgc gggcctcgtg
     1381 gtggtccacg tcgggttcgc gaaggcggcg cggcgggatt gtcagaacct cctgacgacg
     1441 ctggccgacc agcagaaggc ggcgatcgag gcgcacctcg accagcgcat cgcggacgcc
     1501 cgcgtcttcg cagccttccc gagcgtccgc gccctgctgg cctcgggcga cgggctcggc
     1561 gccgaggact ccgcccacct cggggaggtc ctcgaggcgg gccgggccga gtggggagcc
     1621 cgcgccatcc aggtcctcgg cgaggacggg acggtccggg ccggctcggg ggagctgctt
     1681 ccgtccggcg ccgccgcgct cgtcgccggc gccggggaga agccggtcgt cgtcggcctc
     1741 gtcggcgacg gggaggtctc gcgcctcgtc gtggcggcgc gcgtccgcgc gccggacggc
     1801 gccctgcgcg gctgggccgt cttcgtggac gacccggccg ggtcgctctg gaagatcctg
     1861 cggcgtgagc cggtcctctc ccgtacggga gaggcgctcc tcgtctcgac ggaggagggt
     1921 gcgccggtcc ttctctcccc cgcgcgccac atcgtctccg atcagagccg gtcccgcgag
     1981 aggcgtctcg gcccgggccg gggagcgctc gagacgttgg gcggctccgg ccgtttcggc
     2041 gagctcgtcg actaccgcgg cgagacggtc tgggccgcgt tccgccggat cgaccggacg
     2101 cgctggggcc tcgtcgtgaa gatcgaccgg gccgaggcgc tgcagagccg cgcgtcgggc
     2161 cgcctgtggc agtccctcgc tctcgtcttc ctcgctctcg cggccgccgc gaccttccgg
     2221 tcgatccgga cgtcggagcg gctccggacg gcggccgagc tgcagcgccg agacgaacgg
     2281 caccgcctcg tcctggagca ggtgcgtgac gcggtcgtct ggcaccggcc cggagacggc
     2341 cggatcctcg aagcgaacgg cgcggcgacg gagctctggg gcctgactcg tgaagggctc
     2401 ctcgccggaa gcctcttcga cctgcttgcc gcggaggacg tgacggaggc ccggctgaag
     2461 atgtccggcg cgcgccgcgt cggcggcctc atccggacgc gggggaagcg gcgggacgga
     2521 agcctcttcc cggcggacat ctccgcgcgg gccgtcgtcc tcgagggcga ggagattctc
     2581 gtctcggtcg tccgggacgt ctccgccgcc gaggcggctc tcggccagat ccgcttcctc
     2641 aaccgggtcc tccggacgct gacggccgtc gaccaggtcc tcgtcgaggt ccacgaccgc
     2701 gagaccgtcc tccgacggac gtgcgaggag atcgtctcga cgggtggttt cctcgccgcg
     2761 tggttcggcg tcccggatgc ggaagggcgg gtcgttccgg ccgcctcggc cggcgcgctg
     2821 gccggctatc tcgaggaggt gaagatcacg accgacgcgg ggcctctcgg acgcggaccg
     2881 gtcgggaccg cgtttcgcga ggggcggacc gtcgtcgtcg acgactgggc gagcgacccc
     2941 gccgtcgagc cctggaggga agcggcgacg cggagaggtt tccgctccgg agcctcctgc
     3001 gcggtccgga cgggcgagaa gacgatcggc gtcctgacgc tctacgcgga acgcgccggg
     3061 gctttccccc aggaggcggt cctcctcctc gaggagctgg cacgggacct cggactcgcg
     3121 ctcgagctga tcgaggccgg cgagcggcgc cggagggcgg aagagtcgct cgccgcgagc
     3181 gaagcgcgct accggatgct tttcgaggac aaccccggac cgatgtgggt cttcgacgtc
     3241 gagtcccttc gtttcctcgc ggtgaacgag gccgccgtga gcctctacgg ctactcgcgg
     3301 gaggagttcc tggagatgtc gctccgcgag atccggccgg cggaggacga ggcggccctc
     3361 cgggccgacg tgcaggttcc gcggtcggga gtccgctcgt cgggaccgtg gcggcaccgg
     3421 acgaaggacg ggcggatcct gctcgtcgag atcgtcacgc acgacgtcac gtacggggga
     3481 cggccggccc gcctcgttct cgtcaacgac gtgacggagc gcgtccgcgc ggaggagaag
     3541 ctgcgcgcct tcttcgactc cgggatgctg ggcgcgttcc tctccgacgc ggacgggagg
     3601 ctcacggcgg ccaacgacga gttcctccgg atcgtgggac gcaccccggc cgaggtggag
     3661 gagggcgctc tcacctggcg gggcctcacg ccgccggagt ggatcgaggt cgacgtggcg
     3721 agagccgcag aggcgcgggg acgcggcgtc tgcactccct acgagaaaga gtacctgcgg
     3781 aaggacggca gccgggtccc ggtcctcgtc ggtttcgcgc tcgtcgggga gggccgcgac
     3841 gagtcggtgg cgttcgtcct cgaccggacg gaggggaagc gggccggaga ggagctccgg
     3901 cggagcgagg agcggttcac gaagttcctc gactcgcttc cggccgccgc gttcgtgaag
     3961 gcggccgacg ggagggtgct ctgggcgaac cgcttcctcg aggcgctcct cggggccggg
     4021 ccgctggcgg gccggacgac gtacgaggtc ctgccggccg gggcggctgc ggtcatgacg
     4081 gaggacgacc gccgcgccgc cgcgggggag accgtcgagc ggatggagca cctcccggat
     4141 gccgacggcc ggacccgggt ctaccggacg ctcaagttcc cggtgcccgg gcgcgacgga
     4201 gagccgtgga gcggcgggat ctcgatcgac gtcaccgcgc aggtccgcgc cgaggaagag
     4261 gtccgcctcc tgaacgccga gctcgaggcg cgcgtcgagc ggaggaccga ggagctggtg
     4321 gcgaagtcga gggagctgga gagcttcgcc tactcggtct cccatgatct tcgggcgccg
     4381 ctccgcgcga tcgacgggtt ctcccggatg gtcgaggaag accacgccac cgggctcgac
     4441 gcggagggac ggcggctcct cggagtggtc cgggacgccg cgcggaggat ggggcggctc
     4501 atcgacgacc tcctggcgtt ctcccgcgcc gggaggcagg agctcaagag gacgcacgtc
     4561 gacgtcgcgg agctcgtccg aagcgtcgtc ccggaggtcc tccccgaggc ggaacgggag
     4621 aagtccgacg tctcgatcgg cgacctgccg cccgtcgcgg ccgacgcctc gctcctccgg
     4681 caggtcttcg tgaacctcct gtcgaacgcg gtcaagttca gcgcgacgcg aacccggcga
     4741 agaatcgcga tctccgggcg ccgcgaggcg ggccgggtgg actacgatgt ggaggacaac
     4801 ggggtgggtt tcgacatgga gtacgcgggg aagctcttcg gcgtctttca gcgcctccac
     4861 gggagggagt tcgacggaac gggcgtcggg ctcgcgctcg cggagcggat cgtctcccgc
     4921 cacggcggct cgatctcggc ctgggcggaa ccggatcgcg gagcccggtt cacggtgtcg
     4981 atccccgaga caggaggaga ggaatgacgg tgcaggaagc tccggacgtc ctcgtggtcg
     5041 aggacaaccc cgacgacgtc gagctgaccc tccgcgccct gcggcggatg cacctgacga
     5101 atcccgtgcg ggtcgcccgc gacggggtcg aggcgctcga ggtcctcctc ggcgacggca
     5161 cgacccggcc gccgctgccc cgggtcgtgc tgctcgacct caagctcccc cgcgtgggcg
     5221 gcctcgaggt tctcgcccgc ctccgccagg aggaacggac ccggactctc cccgtggtcg
     5281 tcctcacctc ctcgcgcgag gagccggacg tgaagcaggc ctatgccctc ggcgtcaaca
     5341 gctac
//
LOCUS       DAOTEZ010000309         5335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_11122_length_5335_cov_5.621591, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000309 DAOTEZ010000000
VERSION     DAOTEZ010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11122_length_5335_cov_5.621591"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="P5164_14535"
     CDS             <1..399
                     /locus_tag="P5164_14535"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777396.1"
                     /note="catalyzes the formation of glycerone phosphate and
                     D-glyceraldehyde 3-phosphate from D-fructose
                     1,6-bisphosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HRY45159.1"
                     /translation="QANHLGVTIEADIIKQKLPETNGGYLALNSKENPYGKTDKRIYE
                     KLTTDHPIDLTRYQVANCYMGRAGLINSGGASGENDFAEAVKTAVVNKRAGGMGLISG
                     RKAFQRPMAEGVKLLNAIQDVYLCKEVTVA"
     gene            500..1810
                     /locus_tag="P5164_14540"
     CDS             500..1810
                     /locus_tag="P5164_14540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRY45160.1"
                     /translation="MKPFRLAWPPFVAPAEPELLKRQETVLLVLNLTVLAGIAVAYLV
                     FGAVGMAGPPRRTFFAVLMGWFLVQALALGWLAGRGGPIRGAPLLSWVSLSIWLNLAF
                     AFALALSGGMEDTHTAVLLVIPVIAAAFRYTGPGVVLVVAAAVAVTYLQLWVYYSHQP
                     TVAPTEYFEATNAAMVYVVVAIVVASLVRQMRGGAEKLRASLAELEATRDRLVEEEKL
                     AAVGRLAGAIAHEVRNPVTMIVSALGLAADEGTDEAKRAEWHAIAGEEARRLEALTQD
                     FLAYARQKEPDRQSTRLGEEVGYVASLARAKEASTGVRLRVDGPEDAPLSADSFQLHA
                     ALLNLLVNAFDATPPGGEVVLGARPAPGGGATFRVENTGPAIPEEAVPRLFEPFFTTR
                     PRGTGLGLAIARTIARSHGGDVTLAANEEGRVRFELTVGPGPGA"
     gene            1813..3219
                     /locus_tag="P5164_14545"
     CDS             1813..3219
                     /locus_tag="P5164_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRY45161.1"
                     /translation="MARLLVVDDEANMRRVLASILAADGHEVVEADGVRAAQRAFAES
                     PFDLVLTDQRMADGDGLALLASCREVDPTVPVVVMTAYATVELAVEAMKIGAFDFVPK
                     PFQPDAVRAVVRRACERTELVRENERLRGQVRRLAQPAGLLGGSSAMRAVHDLVARVA
                     PTNATVLVQGETGTGKELVARAIHDASPRAARPFVAVNCAGFAETLLESELFGHERGA
                     FTGADRARQGVFEAAHTGALFLDEAGEMGLPLQAKLLRVLTNGEVVRVGATAPRRVDV
                     RIVAATNRDLNAMVAAGTFREDLYYRLAVFPIAVPPLRERREDVPLLAAHFLALAGRE
                     LNTPARSLSAGAIARLAAYGYPGNVRELRNLIERATILARGAVIEADDLPLGPAPEPE
                     AAEEALFRGLPESVELGPTLHLIERRLVQRALASAHGVQAEAARRLGISRSLLAYKLK
                     ALGIAPEAPAAEVSVKKS"
     gene            3396..4037
                     /locus_tag="P5164_14550"
     CDS             3396..4037
                     /locus_tag="P5164_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HRY45162.1"
                     /translation="MKTTSFVRSLPAFALAGAAALVLALPAPAAAPPAASTLDNLQAA
                     YDGESNASAKYAAFAKKADEEGYGQVASLFRAAARAEQIHAANHAAVIKKLGGVPKND
                     VKAPEVKTTKENLQAAIAGESYERDKMYPEFLAKAKKDTNRDAIETFNFAKAAEAEHA
                     KLYSESLAKLDSLKGTKAVTYYVCTVCGFTTTKLDFQKCPVCFNPKDKYTKVG"
     gene            4102..4629
                     /locus_tag="P5164_14555"
     CDS             4102..4629
                     /locus_tag="P5164_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45163.1"
                     /translation="MIELPPAPSWDGLHPLIVHFPVALLLVAPVLVLLALVVRGHRRG
                     LLLAALALMVLGTAASWVAVSTGEAAGKLADRSPEVNAVLEHHEELAETTRLVFTVLT
                     AVFAALVLAPVVLKREPGEVATIGVAVLFLAVYGAGTLVLANTAHNGGRLVHELGVRA
                     LMPPAPLPAAEAEKD"
     gene            complement(4718..>5335)
                     /locus_tag="P5164_14560"
     CDS             complement(4718..>5335)
                     /locus_tag="P5164_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45164.1"
                     /translation="VDLYLSVAEPILVPRTDGPFTLYDQRSCRVQLMVDVPKAVLKSG
                     RLAEVEAALLESVERHETEASARRSEAWNRRERDPYPRDYEETLARHAAWKAEEVNRA
                     LAATRLAALDEAAQALSRVTDDPDYLAGFAAGVEAQRRRSAPGCSSLAGAGFSGEEER
                     APGDRRGSSPAERSFQRGFRDGQVVAWATRVARAVEGCFVPVPGY"
BASE COUNT          751 a         1994 c         1893 g          697 t
ORIGIN      
        1 caggcgaacc acctcggcgt gacgatcgag gcggacatca tcaagcagaa gctccccgag
       61 acgaacggcg ggtacctcgc gctgaactcg aaggagaacc cctacggcaa gaccgacaag
      121 cggatctacg agaagctgac gaccgaccac ccgatcgacc tgacgcgata ccaggtggcg
      181 aactgctaca tgggccgcgc gggccttatc aactcgggcg gcgcctcggg cgagaacgac
      241 ttcgccgagg ccgtgaagac ggccgtcgtc aacaagcgcg ccggcggcat gggcctcatc
      301 tcgggccgca aggccttcca gcgcccgatg gccgaagggg tcaagctcct caacgccatc
      361 caggacgtct acctctgcaa ggaggtcacg gtcgcgtagc gcggtcgcgg cgaactcgtc
      421 gagagccgaa ggcccccggg cgaccggggg ccttcgtgcg taatccgcga tggcccggtg
      481 ctaggctcgc cgacggccca tgaagccctt ccgcctggcg tggcccccgt tcgtcgcccc
      541 ggccgagccc gagctcctca agcgccagga gacggtcctc ctcgtcctca acctgaccgt
      601 cctcgcgggg atcgcggtcg cctacctcgt cttcggcgcg gtcggaatgg ccggccctcc
      661 gcggcggacc ttcttcgccg tcctcatggg ctggttcctc gtgcaggcgc tcgccctcgg
      721 ctggctcgcg gggcggggcg ggccgatccg gggcgccccg ctcctctctt gggtcagcct
      781 ctccatctgg ctcaacctcg ccttcgcctt cgcgctcgcg ctctcgggcg ggatggagga
      841 cacgcacacc gccgtcctcc tcgtcatccc cgtcatcgcg gcagccttcc gctacacggg
      901 gccgggcgtc gtcctcgtcg tcgcggcggc cgtcgcggtc acctatctcc agctctgggt
      961 ctactacagc caccagccga ccgtcgcccc gaccgagtac ttcgaggcga cgaacgcggc
     1021 gatggtctac gtcgtcgtcg ccatcgtcgt cgcctccctc gtgcggcaga tgcggggcgg
     1081 cgccgagaag ctccgggcct cgctcgccga gctcgaggcg acgcgcgacc ggctcgtgga
     1141 ggaggagaag ctcgccgcgg tcgggcgcct cgcgggggcg atcgcgcacg aggtgaggaa
     1201 ccccgtcacg atgatcgtga gcgccctggg cctcgccgcc gacgagggga cggacgaggc
     1261 gaagcgcgcg gagtggcacg cgatcgccgg cgaggaggcc cgccggctcg aggcgctcac
     1321 gcaggacttc ctcgcctacg cgcgccagaa ggagcccgac cggcagtcga cgcgcctcgg
     1381 ggaggaagtg ggctacgtcg cgagcctcgc gagggcgaag gaggcgtcga cgggggtccg
     1441 gctcagggtc gacggaccgg aggacgcgcc gctctcggcc gactccttcc agctccacgc
     1501 cgccctcctc aacctcctcg tcaacgcctt cgacgcgacg cccccgggag gcgaggtcgt
     1561 cctcggggcg cggcccgcgc cgggcggggg cgcgacgttc cgggtcgaga acacgggccc
     1621 cgcgatccca gaggaggccg tccccaggct cttcgagccc ttcttcacca cccgcccgcg
     1681 cgggaccggg ctcggcctcg ccatcgcgcg gacgatcgcg cgctctcacg gcggcgacgt
     1741 cacgctcgcg gcgaacgagg aaggccgcgt ccgcttcgag ctgacggtcg gcccggggcc
     1801 gggggcctga cgatggcgcg cctcctcgtc gtcgacgacg aagcgaacat gcggcgcgtc
     1861 ctcgcgtcga tcctcgccgc ggacggccac gaggtcgtcg aggcggacgg ggtccgcgcg
     1921 gcgcagaggg ccttcgcgga gtcgcccttc gacctcgtcc tgacggacca gcggatggcc
     1981 gacggcgacg gcctcgcgct cctcgcctcg tgccgcgagg tcgacccgac cgtgcccgtc
     2041 gtcgtcatga cggcgtacgc cacggtcgag ctcgcggtcg aggcgatgaa gatcggggcc
     2101 ttcgacttcg tcccgaagcc gttccagccg gacgccgtga gggccgtcgt gaggagggcc
     2161 tgcgagagga ccgagctcgt ccgcgagaac gagcgcctcc gcggtcaggt gcggaggctc
     2221 gcgcagcccg ccggcctcct cggcgggagc tcggcgatgc gggccgtcca cgacctcgtc
     2281 gcgcgcgtcg cgccgacgaa cgcgaccgtc ctcgtccagg gcgagacggg gacgggcaag
     2341 gagctcgtcg cccgggcgat ccacgacgcg agcccgcgcg cggcccggcc gttcgtcgcg
     2401 gtcaactgcg ccggcttcgc cgagaccctc ctcgagagcg aactcttcgg gcacgagcgg
     2461 ggcgctttca ccggggcgga ccgcgcccgg cagggggtct tcgaggcggc gcacacgggg
     2521 gcactcttcc tcgacgaggc gggcgagatg ggcctcccgc ttcaggccaa gctcctccgc
     2581 gtcctgacga acggagaggt cgtccgcgtc ggcgccacgg cgccgcggcg ggtcgacgtg
     2641 cggatcgtcg ccgccacgaa ccgcgacctc aacgcgatgg tcgcggccgg caccttccgg
     2701 gaggacctct actaccgtct cgccgtcttc ccgatcgcgg tgccgccgct ccgcgagagg
     2761 agggaggacg tgccgctcct ggcggcgcac ttcctcgccc tcgcgggccg cgagctgaac
     2821 acgccggccc gctcgctctc cgcgggtgcg atcgcccgcc tcgccgccta cggctatccg
     2881 gggaacgtga gggagctccg aaacctgatc gagcgcgcga cgatcctcgc gcgcggcgcg
     2941 gtcatcgaag ccgacgacct ccccctcggc cccgcgcccg agccggaggc cgccgaggag
     3001 gcgctcttcc gcggcctgcc ggagtccgtc gagctcggcc cgaccctcca cctcatcgaa
     3061 cggcggctcg tgcagcgcgc cctcgcgtcg gcccatggcg tgcaggccga ggcggcccgc
     3121 cgcctcggca tctcgagaag cctcctcgcc tacaagctga aggcgctcgg catcgcgccg
     3181 gaggcgccgg ccgccgaggt gtccgtcaag aaatcttgac gagaacgggc gcccgcgttc
     3241 aagacgtttg acgccggtcg gccgggcgag gcccaccgcg cgaggcatcc ccctcgcgtg
     3301 gaatcacttc tggaatctcc gaagtgtggc accgggcttg cgctccgttc ggagtcggcc
     3361 gggcaagacc tccggctgca cggaggaaat cgaaaatgaa gaccacgtcg ttcgtccgct
     3421 cgctcccggc tttcgccctc gccggcgccg ccgcgctcgt cctcgctctt cccgcgcccg
     3481 ccgccgcccc gccggccgct tccaccctcg acaacctgca ggccgcctac gacggcgaga
     3541 gcaacgcgag cgcgaagtac gccgccttcg cgaagaaggc cgacgaggag ggctacggcc
     3601 aggtcgcgag cctcttccgg gccgccgccc gcgcggagca gatccacgcc gccaaccacg
     3661 cggcggtcat caagaagctc gggggcgtcc cgaagaacga cgtgaaggcc cccgaggtga
     3721 agacgacgaa ggagaacctc caggccgcga tcgcgggcga gagctacgag cgcgacaaga
     3781 tgtacccgga gttcctcgcg aaggcgaaga aggacacgaa ccgcgacgcc atcgagacct
     3841 tcaacttcgc caaggccgcg gaggccgagc acgcgaagct ctactccgag tcgctcgcga
     3901 agctcgattc cctcaagggc acgaaggccg tcacctacta cgtctgcacc gtctgcggat
     3961 tcacaacgac gaagctcgac ttccagaagt gcccggtctg cttcaacccg aaggacaagt
     4021 acaccaaggt cggctgaacg gcccgcgggg cgcggctggc ccaccggcgc cggccgcgct
     4081 tccgccgtcg cggaggacgt gatgatcgag ctcccgcccg ctccgtcctg ggacggactc
     4141 caccccctca tcgtccactt tccggtcgcg ctcctcctcg tcgcgccggt cctcgtcctg
     4201 ctcgcgctcg tcgtccgggg ccaccgccgg ggcctgctcc tcgcggcgct cgcgctcatg
     4261 gtcctcggca cggcggcgtc gtgggtcgcc gtctcgaccg gcgaggccgc cgggaagctc
     4321 gccgaccgga gcccggaggt gaatgccgtc ctcgagcacc acgaggagct cgccgagacg
     4381 acgcggctcg tcttcaccgt cctgacggcc gtcttcgccg ccctcgtcct cgcgcccgtc
     4441 gtgctgaagc gggagccggg cgaggtcgcg acgatcgggg tcgcggtcct cttcctcgcg
     4501 gtctacggcg ccgggacgct cgtcctcgcg aacacggcgc acaacggcgg ccgcctcgtt
     4561 cacgagctcg gcgtccgcgc cctcatgccc ccggcgccgc ttcccgcggc ggaggcggag
     4621 aaggactgac cgggagcccg ggcgcggagc gagggccgct ccgggcgatc gaagatggag
     4681 atgggcgggg ctccggcgcg cccgaaacgg cgggggatca gtagcccggg accggcacga
     4741 agcacccctc gacggcgcgc gcgacgcgcg tcgcccacgc gacgacctga ccgtcgcgga
     4801 agccgcgctg gaagctgcgc tcggcggggg agctgccgcg ccggtcgccc ggcgcgcgct
     4861 cctcctcgcc ggagaagccg gcgccggcga gggaggagca gccgggcgcg gagcgacggc
     4921 gttgcgcctc gaccccggcg gcgaagccgg cgaggtagtc ggggtcgtcg gtcacgcgcg
     4981 agagcgcctg cgcggcctcg tcgagcgcgg cgagccgcgt cgcggcgagg gcacggttca
     5041 cctcctcggc cttccaggcc gcatggcggg cgagagtctc ctcgtagtcg cgcgggtagg
     5101 ggtcgcgttc gcgccggttc caggcctcgg agcgccgagc cgacgcctcg gtctcgtggc
     5161 gttcgacgga ctcgaggagg gccgcctcga cctcggcgag gcggcccgat ttcaggacgg
     5221 ccttcgggac gtcgaccatg agctgcaccc ggcaggagcg ctggtcgtag agcgtgaacg
     5281 gcccgtccgt gcgcggcacg aggatcggct cggcgacgga gaggtagagg tcgac
//
LOCUS       DAOTEZ010000310         5303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_11214_length_5303_cov_7.326791, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000310 DAOTEZ010000000
VERSION     DAOTEZ010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11214_length_5303_cov_7.326791"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1739)
                     /locus_tag="P5164_14565"
     CDS             complement(<1..1739)
                     /locus_tag="P5164_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HRY45165.1"
                     /translation="MHPSKVLPVIPLRNAVLFPGVSFPIAAGRPGTLHAIETAMKSPE
                     QLVFAVTQRQDLEAVTPELLHTVGTIATIGPIQRGVGGARLLLQGKARGIAMRIEEKE
                     GRLEAVVVEAEEMAPLDSTDAAFVALLKELRERSAELAKKLGLPDEAVGNVLTDADDP
                     GRLADLVAGYIDIPTAERQLLLETLPVEDRVRRTLVHVQRQIDVLDAQEEIKSKVQEE
                     LGDRQREMFLRQQLKAIQKELGEGEDDGDDLKALKEKLDALVLTEEVRKEIDREWKKL
                     QRLSRESMEAQVVRNYLELVAELPWSARSEEHLDVVEAQRILDEDHYALKDVKDRILE
                     FLAVRQLLGRKKAEPAAAPSADGAPADAPSAGGAPAPAPAGEKQPNRGRILLFAGPPG
                     VGKTSIAKSIARALGRKYVRISLGGARDEADIRGHRRTYVGAMPGRILQGMRQAGTKN
                     PVFLLDEVDKLGVSFQGDPASALLEVLDPAQNDSFTDHYLNVPFDLSEVLFVATANFV
                     QSIPGPLLDRLEVIEFSGYTEREKTLIARNYLLPRQLAENGLGTDQLEVTDAALAEII
                     SSYTREAGVRQLER"
     gene            complement(1943..2320)
                     /locus_tag="P5164_14570"
     CDS             complement(1943..2320)
                     /locus_tag="P5164_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008085219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HRY45166.1"
                     /translation="MRIARSASFKATVERSRFFAVAFPAATEEDVLHAVKSARSEHRK
                     ANHHCWAFRGRDVAGALVERSKDDGEVGHPGRLLLELLRRDDLEGGLLVSRVFGGVKL
                     GVGGVSRAFREAAEGALESLKSP"
     gene            2360..3955
                     /locus_tag="P5164_14575"
     CDS             2360..3955
                     /locus_tag="P5164_14575"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRY45167.1"
                     /translation="MRRATIPPAAVAALLAFLLAHRGEAAGPPCGTLAQLRSRLAGAP
                     DDAARTTAEAEFSACAARHGTPLLSDGSAPGRVRALFAFRSPAEKVFLAGDMSGWSTS
                     AQPLERLTGTDLHVLEMDVPDGARLDYKLVVSGTEWTLDPWNPKTMTGGYGPNSELRT
                     PAYVPPAGVEPGPGTVKGAWEELKVESRAMGGARSAFVWSSAPAGATGPYPVLYLLDG
                     TDYRDFAKAHVVVGNLVGAGRLPPVVLVLVPPVDRRAEYDDDRSAPGGRGTDRPQRRE
                     ATAIAPTHAAFTRFLVDELVPAVEARWPVRRDAAGRGVMGASLGGFAALSITARRPGT
                     FGRCGAQSTGGGDGGGFEALLADLGRLPAGSLRVHLDAGTFEALFHGFDLLSLTRHLR
                     AELSRRQVVQYREVPEGHSWGSWRARLADALTFFWGDESRAQPLSARPLAAPPSGAAR
                     FAGEPVSLDVKDAELGDVVRRLTEGRGLSVVAPPSFRGTVTASLRDVPWDQALDLVLA
                     GNGWAFLREGTVIRVVRRDDPGR"
     gene            complement(3966..>5303)
                     /locus_tag="P5164_14580"
     CDS             complement(3966..>5303)
                     /locus_tag="P5164_14580"
                     /inference="COORDINATES: protein motif:HMM:NF015148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HRY45168.1"
                     /translation="VFGLGYIMGLDYAAIIMAGSMVSFLVLVPLFAWLSQWVPGAISA
                     GAPALAAMPAEDIFFEYVRPIGIGGIFCAGLLSILKMSPVIVQATRQAFGEVARLARG
                     GGAAAEAERTDRSLPMWVVLAGIVATALAILLYFRLSVLAGAPGATRLALVSTGLTLL
                     ISFLFAAVSAWAVAMISITPVSGMTLTTLIVTAVVLSSLGLSGQDGMLQTLLVGGVVC
                     TALSMSASLVTQYKIGYWLGATPRRIEVWNLLGSVAASAATTAVILLMAKVYGFAPST
                     LHANPLPAPQPNAMAAVLRGVMGDTGAPWFLYAVGAVFAVVVELCGVSGLAFALGMYL
                     PMELNSPLVLGAAVAWLVRRSTPDEPLAKARHEKGTLIASGFIAGGALVGVLAALLRF
                     VEDSTGRTLVPDLTAVPGVGAWLSAWGNWLGLAVFLALAAFVYRSAKRETVEG"
BASE COUNT          747 a         1917 c         1907 g          732 t
ORIGIN      
        1 cgctcgagct ggcggacgcc cgcctcgcgc gtgtaggacg agatgatctc cgcgagcgcc
       61 gcgtcggtca cctcgagctg gtcggtcccc aggccgttct ccgcgagctg cctcgggagg
      121 aggtagttcc gcgcgatgag ggtcttctcc cgctccgtgt agcccgagaa ctcgatcacc
      181 tcgaggcggt cgaggagcgg ccccgggatc gactggacga agttcgccgt cgcgacgaag
      241 aggacctcgg agaggtcgaa cgggacgttc aggtagtggt cggtgaagct gtcgttctgg
      301 gccgggtcga ggacctcgag gagggccgag gccgggtcgc cctggaacga gacgccgagc
      361 ttgtcgacct cgtcgaggag gaagaccggg ttcttcgtcc cggcctggcg catcccctgg
      421 aggatccgcc cgggcatcgc gccgacgtag gtccgccgat ggccgcggat gtccgcctcg
      481 tcgcgcgcgc cgccgaggga gatcctcacg tacttgcgtc cgagggcgcg ggcgatcgac
      541 ttcgcgatcg aggtctttcc gaccccggga gggcccgcga agaggaggat gcgcccgcgg
      601 ttcggctgct tctcgccggc cggcgccggg gcgggcgccc cgccggcgga gggagcgtcc
      661 gccggcgcgc cgtccgcgga cggcgcagct gcgggctccg ccttcttccg accgaggagc
      721 tgacggacgg cgaggaactc gaggatccgg tccttcacgt ccttgagggc gtagtggtcc
      781 tcgtcgagga tccgctgcgc ctcgacgacg tcgaggtgct cctcgctccg cgccgaccac
      841 ggcagctcgg ccacgagctc gaggtagttc cgcacgacct gggcttccat cgactcgcgc
      901 gaaaggcgct ggagcttctt ccactcccgg tcgatttcct tgcggacctc ctcggtcagg
      961 acgagcgcgt cgagcttctc cttcagggcc ttcaggtcgt cgccgtcgtc ctcgccctcc
     1021 cccagctcct tctggatcgc cttcagctgc tggcggagga acatctcgcg ctggcggtcg
     1081 ccgagctcct cctggacctt cgacttgatc tcctcctgcg cgtcgaggac gtcgatctgc
     1141 cgctgcacgt ggacgagcgt ccggcggacc cggtcttcga ccggcagcgt ctcgaggagg
     1201 agctgccgct ccgcggtcgg gatgtcgatg tagccggcca cgaggtcggc gagacggccc
     1261 ggatcgtcgg cgtcggtaag gacgttcccg acggcctcgt ccggcaggcc gagcttcttc
     1321 gcgagctcgg cggagcgctc ccgcagctcc ttcagcagcg cgacgaacgc cgcgtcggtg
     1381 gagtcgagcg gggccatctc ctccgcttcc acgacgacgg cctcgaggcg tccctccttc
     1441 tcctcgatcc gcatcgcgat tccgcgcgcc ttcccctgga ggaggagccg cgcgccgccg
     1501 acgccgcgct ggatcggccc gatcgtggcg atcgtcccga cggtgtggag gagctcgggc
     1561 gtcaccgcct cgaggtcctg cctctgcgtg acggcgaaga cgagctgctc ggggctcttc
     1621 atcgcggtct cgatcgcgtg gagggtcccg gggcggccgg cggcgatcgg gaagctgacg
     1681 ccgggaaaga ggactgcgtt gcggagcggg atgaccggga ggaccttcga tgggtgcatc
     1741 tggacctttc gagccaacct aaagtgctgg actcaagaat aaactttagt aaatacggct
     1801 tagattttca agccacgcgg tgaacggagg tatgtcactc atgttgagta gtttgacctg
     1861 ggcccccgtc gtcggggacg aagtccgttc cacggcagaa ccgagtccac caacgccggt
     1921 attccccggg aagagcccgg cgctagggcg acttcaggct ttcgagcgct ccctcggccg
     1981 cttcgcggaa cgcccgggag acaccgccga cgccgagctt cacgccgccg aagacccggg
     2041 agacgaggag tccgccctcg aggtcgtcgc gcctgaggag ctcgaggaga agtcggcccg
     2101 ggtgcccgac ttccccgtcg tccttcgacc tctcgacgag cgcccccgcg acgtcgcggc
     2161 cgcggaaggc ccagcagtgg tggttcgctt tccggtgctc gctcctcgcg gacttcacgg
     2221 cgtggaggac gtcctcctcc gtcgccgccg ggaaggcgac ggcgaagaag cgcgaccgct
     2281 cgacggtcgc cttgaaggac gcggagcggg cgatcctcac gcgtcgaact tagcaggagc
     2341 ggccggagaa tgggcctgta tgcgcagggc cacgatcccg ccggccgccg tcgcggcgct
     2401 cctcgcgttc ctcctcgccc atcgcggcga ggccgccggc cccccttgcg ggacgctggc
     2461 ccagctccgg tcgcggctcg ccggtgcacc cgacgatgcg gcccgcacga cggccgaagc
     2521 cgagttctcc gcgtgcgccg cccggcacgg gactcccctg ctctcggatg gaagcgcgcc
     2581 gggccgggtc cgcgccctct tcgccttccg ctcgcccgcg gagaaggtct tcctcgcggg
     2641 cgacatgagc ggctggagca cgtcggcgca gcccctcgag aggctcacgg gtaccgacct
     2701 ccacgtcctc gagatggacg tccccgacgg cgcgcgcctc gactacaagc tggtcgtctc
     2761 gggaacggag tggacgctcg acccgtggaa cccgaagacg atgaccggcg gctacggccc
     2821 gaactccgag ctccgcaccc cggcgtacgt cccgcccgcc ggcgtcgaac cgggccccgg
     2881 cacggtcaag ggggcctggg aggagctgaa ggtcgagagc cgcgcgatgg gcggagcgcg
     2941 aagcgccttc gtctggtcgt ccgcccccgc gggcgcgacg ggcccgtacc ccgttctcta
     3001 cctcctcgac ggcacggact accgcgactt cgcgaaggcc cacgtcgtcg tcgggaacct
     3061 cgtcggtgcg gggcggctcc cgcccgtcgt cctcgtcctc gtcccgcccg tcgaccggcg
     3121 cgcggagtac gacgacgacc gctcggcgcc gggcggacgc ggaacggacc ggccgcagcg
     3181 cagggaggcg accgcgatcg ccccgacgca cgcggccttc acgcgattcc tcgtcgacga
     3241 gctcgtcccc gccgtcgagg cccgctggcc cgtgcggagg gacgccgcgg ggcgcggcgt
     3301 gatgggggcc tcgctcggcg gcttcgccgc gctctcgatc acggcacgcc gcccgggcac
     3361 cttcggccgc tgcggtgcgc agtcgaccgg gggcggcgac ggtggaggct tcgaggcgct
     3421 cctcgccgac ctcgggaggc ttccggcggg aagcctccgc gtccacctcg acgccgggac
     3481 gttcgaggcg ctcttccacg gcttcgacct cctctcgctc acgcggcacc tgcgcgccga
     3541 gctctcgcgg cgccaggtcg tccagtaccg cgaggtcccc gaggggcaca gctggggctc
     3601 gtggcgcgcc cgcctcgccg acgcgctgac gttcttctgg ggcgacgaga gtcgggctca
     3661 gcctctctcc gcccgcccgc tcgccgcgcc cccgtccggg gccgcccggt tcgcgggcga
     3721 gcccgtctcg ctcgacgtca aggacgcgga gctcggcgac gtcgtccggc ggctgaccga
     3781 ggggcgcggc ctctccgtcg tcgcgccgcc gtccttccgc gggaccgtca ccgcctcgct
     3841 gcgcgacgtc ccgtgggatc aggcgctcga cctcgtcctc gcggggaacg gctgggcgtt
     3901 cctccgcgag gggaccgtga tccgggtcgt ccgccgcgac gaccccggcc gctgacgtcc
     3961 cgccgtcagc cctccaccgt ctcccgcttc gcgctccggt agacgaacgc cgcgagggcg
     4021 aggaagacgg caaggccgag ccagttcccc cacgcggaga gccacgcgcc gaccccgggg
     4081 accgccgtca ggtcggggac gagcgtccgc cccgtcgagt cctcgacgaa cctcagaagc
     4141 gccgcgagaa ccccgacgag ggcgccgccc gcgatgaagc ccgaggcgat gagcgtcccc
     4201 ttctcgtggc gcgccttcgc gagcggctcg tcgggcgtcg accgcctcac gagccaggcc
     4261 acggccgcgc cgaggacgag cggcgagttc agctccatcg gcaggtacat cccgagcgcg
     4321 aaggcgaggc ccgagacgcc gcacagctcg acgacgaccg cgaagacggc gccgacggcg
     4381 tagaggaacc acggggcccc cgtgtcgccc ataaccccgc ggaggaccgc ggccatcgcg
     4441 ttcggctgcg gcgctgggag cgggttggcg tggagcgtcg acggcgcgaa gccgtagacc
     4501 ttcgccatca ggaggatgac ggccgtcgtc gccgccgagg cggcgacgga gccgaggagg
     4561 ttccagacct cgatccgccg cggcgtcgcg ccgagccagt agccgatctt gtactgcgtg
     4621 acgagcgagg cgctcatcga gagggccgtg cagacgacgc ccccgacgag gagcgtctgg
     4681 agcatcccgt cctggccgga gaggccgagc gaggagagga cgacggccgt gacgatgagc
     4741 gtcgtgagcg tcatccccga gacgggcgtg atcgagatca tcgcgacggc ccaggcggag
     4801 accgccgcga agaggaacga gatgaggagc gtgaggccgg tggagacgag cgcgaggcgc
     4861 gtggcgcccg gcgcgcccgc gaggacggag agccggaagt agaggaggat cgcgagcgcc
     4921 gtcgcgacga tcccggcgag gacgacccac atcgggagcg accggtccgt ccgctcggcc
     4981 tcggccgccg ctccgccgcc ccgtgcgagg cgcgcgacct cgccgaacgc ctgccgcgtc
     5041 gcctggacga tgacgggcga catcttcagg atcgagagga gcccggcgca gaagatgccg
     5101 ccgatgccga tgggccggac gtactcgaag aagatgtcct ccgccggcat cgcggcgagc
     5161 gcgggcgccc ccgccgagat cgcccccggg acccactgcg acagccacgc gaagagcggg
     5221 acgaggacga ggaaggagac catcgacccc gccatgatga tcgccgcgta gtcgagcccc
     5281 atgatgtagc cgaggccgaa gac
//
LOCUS       DAOTEZ010000311         5298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_11227_length_5298_cov_5.989510, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000311 DAOTEZ010000000
VERSION     DAOTEZ010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11227_length_5298_cov_5.989510"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="P5164_14585"
     CDS             complement(<1..231)
                     /locus_tag="P5164_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRY45169.1"
                     /translation="MESRTLGTQGLVVSAQGLGCMGMSEFYAGRDDEESVATIHRALD
                     LGVTFLDTADMYGPRTNEVLVGKAIRGRRSEAV"
     gene            476..1852
                     /locus_tag="P5164_14590"
     CDS             476..1852
                     /locus_tag="P5164_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HRY45170.1"
                     /translation="MKLWLRGRSSRLLAATFLLAAGAVARADDRFTLSGDLRLRHTVL
                     ANVGLRDADPNADRTFQRYRLRLRADAALAGPVRATARLLWEGRHYAKPDGAAWPVSG
                     FESWYGGALLFDLLALEVKAPGGLPFVARVGRQEILLGDGWLVQDGTPIDGSRTGYFD
                     AARLTFGAASAGRTIDLVWLDQSADTGRFPRPLLGGTEDQLEQGERGAVLWAHDERLV
                     KRTKLDAYVVWKDARANAAPGNVRVNNGAPFPSPSDSGEVLTLGGRVEVVPSPGWELK
                     AEGAAQSGRRNGRDLRSFGVTGRVTRRFGRGRKHRAHVGFEHLSGDDPASGRDEAFDP
                     LWGRWPQWSELLIYQWPLDSRVGEATNVRRLGAGLLLQLGADSSLALDLHLLGADERS
                     ARFAAQAANLGGGRDRGLLVTGRWRRAFDAHVSAHLHAEYFRPGDFYAAHRRDESYFA
                     RAELNLAF"
     gene            1926..2168
                     /locus_tag="P5164_14595"
     CDS             1926..2168
                     /locus_tag="P5164_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0106034 - protein maturation by [2Fe-2S]
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HRY45171.1"
                     /translation="MSEPKIAAKEPVRVSLEAGKTYAWCRCGLSKSQPFCDGSHRGTE
                     LAPLVWKAEEAKEAWLCQCKRTGNAPFCDGTHKKLP"
     gene            2246..2731
                     /locus_tag="P5164_14600"
     CDS             2246..2731
                     /locus_tag="P5164_14600"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HRY45172.1"
                     /translation="MGSLDGIEVRTIDGKAETLAKHAGKALLVVNVASKCGFTPQYAG
                     LEALWKKYRDRGLVVLGFPCDQFGHQEPGSEAEIQAFCSRTYGVTFPMFAKVEVNGDG
                     AHPLYRHLKKAAPGLLGTEAIKWNFTKFLVAPGGAPVTRFAPNDAPESLAAAIEKALP
                     G"
     gene            complement(2739..4319)
                     /locus_tag="P5164_14605"
     CDS             complement(2739..4319)
                     /locus_tag="P5164_14605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY45173.1"
                     /translation="MTRVLVVDDESQNRYLLEVLLGGEGLEVESASNGREALAAARRR
                     APDLVISDILMPEMDGFLLCRALKGDASLRNVPFVFYTATYTEVEDERVALEAGGDLF
                     LRKPTEPDTLLAAIRRLLEERAPASDDARTGGLAEEVAFLARHDKALARKLEKKHHEL
                     QESEARYRSYFRSSPVAILVTDDAGRVLDANPAASLLSGYDPVELLGANVSILLGEGG
                     GDTPLRLAAGAPSAPSRTTEWRLRRKDGTPRHVSLTAIRLEDGRVLAFCEDDTERVRA
                     QDAVLKANAELETRVRERTAQLEHLNAELEAFTSSVSHDLRAPLRALDGYAAMLEEEV
                     EDRPESDCRRHVDAIRRNVRRMSELVESLLTLSRAGRQPLRTGPIDMERLARSVLDEL
                     LVPEEREKTEVVFRPLPAAVGDESLVRQVLANLVGNALKFSARKERRRLEIGARNEGG
                     RTCWFVSDDGIGFEPAHAEKIFRIFERLPGAEEFEGTGVGLAVVRRIVERHGGVVRAE
                     GTPGKGATISFSLEEGAG"
     gene            complement(4316..4696)
                     /locus_tag="P5164_14610"
     CDS             complement(4316..4696)
                     /locus_tag="P5164_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY45174.1"
                     /translation="MSGNVLVVEDNEQNLYLVRFLLEGAGWHVVAAPDGPRALEEARS
                     GRFELVLLDIQLPGMDGHAVARELRRIPALDGVPIVAVTSYAMLGDRERCLEAGCTGY
                     VEKPINPDTFLDEVVSYVRPEVRP"
     gene            complement(4693..>5298)
                     /locus_tag="P5164_14615"
     CDS             complement(4693..>5298)
                     /locus_tag="P5164_14615"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY45175.1"
                     /translation="TEEQSRQLGMVKSSAQHLLALINDVLDLSKIEAGQLRVSSAPFA
                     LTEAVEGCLRALAPAAERKGLSLVAEVDPAIGTVRSDRRRVEQVLLNLVSNAVKFTET
                     GSVTVSAVREGPWVVTRVRDTGIGISAEDLPNLFRPFQQLESGLTRRFEGTGLGLSIC
                     RRLAEMLQGEITVESAPGAGSTFSFRLPAGEAPADIGTEGR"
BASE COUNT          734 a         1864 c         1954 g          746 t
ORIGIN      
        1 gacggcctcg ctccgccggc cgcggatcgc cttgccgacg aggacctcgt tcgtgcgcgg
       61 gccgtacatg tcggccgtgt cgaggaacgt gacgccgaga tcgagcgcgc ggtgaatcgt
      121 cgcgaccgac tcttcgtcgt cgcgccccgc gtagaactcc gacatcccca tgcagccgag
      181 gccctgggcg gagacgacga ggccctgcgt cccgagagtg cgtgattcca tgggcgacct
      241 cccgcgcggg ccggagggga cgacgccctc gcccgcgcgg agggaccgta gcacgcggga
      301 cgtgccggag agccgacttc gcccgtcccc gctcgggctg accacggagc cgtccgcgcc
      361 ggtcgggact gcccgccgcg ggagatcgcc gcgccgggag atcctcgctt gtctcgcccg
      421 cttcgcgacc ctatcctgac gcgatggctt gcaggacgcc ggggcggacc cggccgtgaa
      481 gctctggctc cgaggccgct cgagccggct gctcgccgcg acgttcctgc tcgcagccgg
      541 cgccgtggcg agggcggacg acaggttcac gctctcgggc gacctccgcc tgcggcacac
      601 ggtcctcgcg aatgtcggcc tcagagacgc cgacccgaac gccgaccgga cgttccagcg
      661 ctatcggctc cggctccgcg ccgacgcggc gctggccggt ccggtccgcg cgaccgcgag
      721 gctcctctgg gaaggccggc actacgcgaa gcccgacggc gccgcctggc cggtgtcggg
      781 cttcgagtcg tggtacggcg gggcgctcct cttcgacctc ctcgctctcg aagtgaaggc
      841 ccccggcggg ctgccgttcg tcgcgagggt cggtcggcag gagatcctcc tcggcgacgg
      901 ctggctcgtt caggacggga cgccgatcga cggctcgcgg acgggctact tcgacgcggc
      961 ccggctcacg ttcggcgccg cctcggcggg aaggacgatc gacctcgtct ggctcgacca
     1021 gtccgcggac accgggcggt tccctcgtcc gctcctcggc gggaccgaag accagctgga
     1081 gcagggcgag cggggggcgg tcctctgggc gcacgacgaa aggctcgtga agcggacgaa
     1141 gctcgacgcg tacgtcgtct ggaaagacgc acgtgcgaac gcggctccgg ggaacgtccg
     1201 cgtgaacaac ggggctccct tcccttcacc ttccgactcg ggtgaggtcc tcacgctcgg
     1261 ggggcgtgtc gaggtcgtgc cgtcgccggg ctgggagctg aaggccgagg gggcggccca
     1321 gagcggccgg cgcaacggcc gcgacctccg atcgttcggg gtcaccggac gggtgacgcg
     1381 gcgattcggc cgggggcgga agcaccgggc gcacgtgggg ttcgagcacc tctcgggcga
     1441 cgacccggcg agcggccgcg acgaggcgtt cgaccccctc tggggccgct ggccgcagtg
     1501 gagcgagctg ctgatctacc agtggccgct cgactcccgg gtgggcgagg cgacgaacgt
     1561 gcggcggctc ggcgcggggc tcctgctgca gctcggggcg gactcgtcgc tcgcgctgga
     1621 cttgcacctc ctcggcgcgg acgagaggag cgcccgtttc gccgcgcagg cggcgaacct
     1681 cggagggggc cgagaccgcg ggctgctcgt cacggggcgt tggcgcaggg cgttcgacgc
     1741 gcacgtctcg gcgcacctcc acgccgagta tttccgcccg ggcgacttct acgccgctca
     1801 ccggagggac gagtcgtact tcgcccgcgc ggagctgaac ctggcgttct gacccggcgt
     1861 gcgagaacgg ccgggcgcga cgctatgatg tcgcggccac gaccgaagga ccgaggagga
     1921 cgccgatgtc cgagccgaag atcgccgcga aggagcccgt ccgcgtgagc ctcgaggcgg
     1981 ggaagacgta cgcctggtgc cgttgcgggc tctcgaagag ccagccgttc tgcgatggct
     2041 cgcaccgggg gacggagctc gcgcccctcg tctggaaggc cgaggaggcg aaggaggcgt
     2101 ggctctgcca gtgcaagcgg accgggaacg ccccgttctg cgacgggacg cacaagaagc
     2161 tcccctgagg gcgagcggcg ccctcgttcc gggccgcgat ccgacctccg gccgggggcg
     2221 tagggggact cgaaggagga gcggcatggg atctctcgac ggcatcgagg tccggacgat
     2281 cgacgggaag gccgagacgc tcgcgaagca cgcggggaag gcgctcctcg tcgtgaacgt
     2341 cgcaagcaag tgcggcttca cgccgcagta cgcggggctc gaggcgctct ggaagaagta
     2401 ccgcgaccgg ggcctcgtcg tcctcggctt cccgtgcgac caattcggcc accaggagcc
     2461 gggaagcgag gcggagatcc aggccttctg ctcgcggacc tacggcgtga cgttcccgat
     2521 gttcgcgaag gtggaggtga acggggacgg cgcccacccg ctctaccggc acctgaagaa
     2581 ggcggctccg gggctgctcg ggaccgaggc gatcaagtgg aacttcacga agttcctcgt
     2641 cgccccggga ggcgcgccgg tgacccgctt cgccccgaac gacgccccgg agagcctcgc
     2701 ggccgcgatc gagaaggctc tcccgggctg aacgcccgtc agccggcgcc ttcctccaga
     2761 gagaacgaga tcgtcgcgcc cttcccgggt gttccctccg cccggacgac gcccccgtgg
     2821 cgctcgacga tccggcgcac gacggcgagc ccgacgccgg tcccctcgaa ttcttcggcg
     2881 ccggggagcc tttcgaagat gcggaagatc ttctccgcgt gcgcgggctc gaagccgatg
     2941 ccgtcgtccg agacgaacca gcaagtccgc cctccctcgt ttcgggcgcc gatctcgaga
     3001 cgcctgcgct ccttccgtgc gctgaacttg agggcgttcc cgacgaggtt cgcgagcacc
     3061 tggcggacga ggctctcgtc gccgacggcc gcagggagcg ggcggaagac gacctcggtc
     3121 ttctctcgct cctcggggac gagcagctcg tcgaggacgg agcgggcgag tcgctccatg
     3181 tcgatcggtc ccgtccggag cggctggcgc ccggccctcg agagggtgag gaggctttcg
     3241 accagctcgg acatccgacg gacgttgcgg cggatggcgt cgacgtgccg gcgacagtcc
     3301 gactccggac ggtcctcgac ctcctcctcg agcatcgccg cgtagccgtc gagcgcgcgg
     3361 agcggcgcac gcaggtcgtg cgacaccgag gaggtgaagg cctcgagctc ggcgttgagg
     3421 tgctcgagct gcgcggtccg ttcccggacc ctcgtctcga gctcggcgtt cgccttcagc
     3481 accgcgtcct gcgcgcggac ccgctcggtg tcgtcctcgc agaacgccag gacgcgtccg
     3541 tcctccagcc ggatcgccgt gagcgagacg tgacgtggcg tgccgtcctt ccgccgaagg
     3601 cgccactcgg tcgtccggga gggggccgac ggcgcccccg ccgcgagccg gaggggcgtg
     3661 tcgccgccgc cttcgcccag caggatcgag acgttcgcgc cgagcagctc cacggggtcg
     3721 tacccggaaa ggaggctcgc cgcggggttc gcgtcgagca cccgtcccgc gtcgtcggtc
     3781 acgaggatcg ccacgggcga gctccggaag tagctccgat agcgcgcctc gctctcctgg
     3841 agctcgtggt gcttcttctc gagcttccgg gccagcgcct tgtcgtgccg ggccaggaac
     3901 gcgacctcct cggcgagtcc ccccgtccgc gcgtcgtcgc tcgcgggcgc cctctcctcg
     3961 aggaggcgcc ggatcgcggc gagcagggtg tccggctccg tcggcttgcg gaggaagagg
     4021 tcccctcccg cctcgagggc gacccgctcg tcctcgacct cggtgtaggt cgcggtgtag
     4081 aagacgaacg gcacgttccg gagggacgcg tcccccttca gcgcgcggca gaggaggaag
     4141 ccgtccatct cgggcatcag gatgtcggag atgacgaggt cgggagcccg gcgccgggcc
     4201 gcggcgaggg cctcgcggcc gttcgaggcc gactccacct cgagcccctc gcccccgagg
     4261 aggacctcga ggaggtatcg gttctgcgat tcgtcgtcca cgacgaggac gcgggtcacg
     4321 gacggacctc cggtcgcacg tagctcacga cctcgtcgag gaaggtgtcg ggattgatcg
     4381 gcttctcgac gtagccggtg cagccggcct cgaggcaccg ttcgcggtcg ccgagcatcg
     4441 cgtacgacgt gacggcgacg atcgggacgc cgtcgagggc cgggatccgg cgcagctccc
     4501 gcgcgacggc gtgcccgtcc attccgggga gctggatgtc gaggaggacg agctcgaagc
     4561 ggccggagcg cgcctcctcg agcgcacgcg ggccgtccgg cgcggcgacg acgtgccagc
     4621 cggccccctc gagcaggaaa cggacgaggt agaggttctg ctcgttgtcc tcgacgacca
     4681 ggacgtttcc gctcatcgcc cctccgttcc gatgtcggcc ggcgcctctc cggcgggaag
     4741 ccggaagctg aacgtgctcc ctgcgccggg agcgctctcg acggtgatct cgccctggag
     4801 catctcggcg agccgccggc agatggagag cccgaggccg gtcccttcga agcgacgggt
     4861 gaggcccgac tcgagctgct ggaaaggccg gaacaggttc gggaggtcct cggcggagat
     4921 gccgatgccg gtgtcgcgga cgcgcgtgac gacccacggc ccctcgcgga cggcgctcac
     4981 cgtaacgctc cccgtctccg tgaacttcac cgcgttggag acgaggttca ggaggacctg
     5041 ctccacgcgc cgccggtcgc tccggaccgt cccgatggcg gggtcgacct ccgccacgag
     5101 ggagaggccc ttcctctcgg cggccggcgc cagggcgcgc aggcatccct cgacggcttc
     5161 cgtcagcgcg aacggcgcgg acgacacgcg gagctggccc gcctcgatct tcgagaggtc
     5221 gaggacgtcg ttgatcaggg cgaggaggtg ctgcgcgctc gacttcacca tcccgagctg
     5281 gcgggactgc tcctcggt
//
LOCUS       DAOTEZ010000312         5279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_11290_length_5279_cov_6.702718, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000312 DAOTEZ010000000
VERSION     DAOTEZ010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11290_length_5279_cov_6.702718"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="P5164_14620"
     CDS             <1..223
                     /locus_tag="P5164_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY45176.1"
                     /translation="DTGIGIRAEDLDKLFRPFRQVDSGTSRHYDGTGLGLSISRKLAE
                     LLGGSIDVESEPGRGSTFTVVLPLTRKAP"
     gene            220..612
                     /locus_tag="P5164_14625"
     CDS             220..612
                     /locus_tag="P5164_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY45177.1"
                     /translation="MKAKILYIEDNTQNFYLVNFILTAAGSEVVWAKDGPEGVEKAAL
                     GWADLILLDIQLPGMDGYAVAQALRGNPALSSTPIVALTSYAMAGDREKALAAGCTGY
                     IEKPIDPGSFAAQVERHLLSARSEGGAS"
     gene            609..1838
                     /locus_tag="P5164_14630"
     CDS             609..1838
                     /locus_tag="P5164_14630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY45178.1"
                     /translation="MSRVLIVDDIVENRYLLETVLKAGGYEVVSAANGAEALETARIA
                     PPDLLVTDILMPVMDGFELCRRWKADEGLRGIPLVFYTATYTDSKDEALARTLGADRF
                     LVKPLPPDTLLEEVRAALETPRAESPAADAGEVLQEYSEALFRKLQRKVAQLEAEAAT
                     RRRAEEEVRALNAELEERVRTRTAELETSIREMEAFSYSVSHDLRAPLRAIDGYSALL
                     ESEASANLDAEGRELLRKVRASARTMSTLIDELLEFSRTGRSELRRTRLDMCEIVRSA
                     FEQVTTPAERERVELRVDALPPADGDAALVRHVVTNLLSNAVKFSSGRERAVIEVGSR
                     REGDRTAYFVKDNGAGFDMRYADKLFGVFQRLHSPAEFPGVGVGLAIVQRIVTRHGGT
                     VSARGRVGEGAEFTFSL"
     gene            complement(1870..3213)
                     /locus_tag="P5164_14635"
     CDS             complement(1870..3213)
                     /locus_tag="P5164_14635"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRY45179.1"
                     /translation="MNAPDGLPPLGEERPVPMPEVAASRLPNGLAVQVVPWRGVPLAS
                     ARLVVRGGLSDDPSGRPGLARLLAAALREGTASRSGADLADLAQEAGGDLSAGAGPDS
                     LTIGAYGLAARLPLLLDLVADVARRAAFPADGVARARHLAHEELETSESEPSFLAVRA
                     FAREVYGTHPYATIAPTAASIDAVTPGLLAAEARRRLRPERALLLVAGDVKPEEAIAA
                     AGNAFGDWAAGGSAPPPVAAPAPTGGPRRILVLDRPGSVQTNLMVGNLGFPRTDRDAR
                     ALELAMTIYGGSFTSRLVQNLREDKGYTYSPGAAASWLEGCGTVRSFAAVRTEVTGAA
                     LNEVLYEMERMATTEATDEEMDRAFRREAGSLAIALQTAAGVADELARLWVLGLSPDE
                     IARFVPGLAEVTKGEVRRVSGRVFGTGSARIVAVGDAAAIRSELGTFGEVVEGET"
     gene            complement(3210..4559)
                     /locus_tag="P5164_14640"
     CDS             complement(3210..4559)
                     /locus_tag="P5164_14640"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRY45180.1"
                     /translation="MHDRRGDLRDPADGHRAAAPEGLTLRVPVETLVLPNGLTVVVSE
                     NRSAPVFGLCVLYRIGSRLEPRGRSGFAHLFEHLMFEGTPNAPKGVFDRVCEASGGSN
                     NGQTRPDVTLYVVDAPSSALERILFLEADRMSGLAFGQESLDNQRDVVKEEILVNVRN
                     DPYGLFEYGELPQALFTKWENAHDGYGDFHDLDAATLADVEGFFSAFYRPDNAVLAVA
                     GDVGADEVFSLAERRFGAIPRGAPYARPDLHEPPREAPVLLVQEAPLARTPAVAMGWR
                     MPERTHADAAGLLVLGELLHNGRASRLYRGLVEGREIATDLSGGFNPFQGGVWYDGTT
                     LFLSRIGYRREVSAEAVLAAFDEEVARIARDGVGEAELARVKTKVLADTWAGLEPRLG
                     LAIELAQAAAIAGAPEALLDLPRLVEAVSPGDLSRAAAWLAPERRAAIVKVPPKGEA"
     gene            complement(4490..>5279)
                     /locus_tag="P5164_14645"
     CDS             complement(4490..>5279)
                     /locus_tag="P5164_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRY45181.1"
                     /translation="MIGAWGLTEAEAGSDAKGTRTTAVLDGNEWVLNGSKNFITHGSV
                     GDVAVLMAVTDRAAGTHGISAFVVDLHQKGIRAGKKENKLGLRASDTATLVMEDCRVP
                     KEALVGPLGSGFTQAMQVLDGGRISIAALALGMAQGAYDCALAYSRQRVQFGKPISEF
                     QSIQNYLADMATEIDAARLLTWRAGWMKDRGMKVTKESAMCKLYAGEVSVRVANLAVQ
                     VHGGYGFTKDFKAEKFYRDVKLCTIGEGTSEIQRMVIARQLLKA"
BASE COUNT          749 a         1902 c         1929 g          699 t
ORIGIN      
        1 cgacaccggg atcgggatcc gcgcagagga cctcgacaag ctcttccggc cgttccggca
       61 agtcgactcc ggcacctccc gccactacga cgggaccggc ctcgggctct ccatcagccg
      121 gaagctggcc gagctgctcg ggggtagcat cgacgtcgag agcgagccgg gccgcggaag
      181 cacgttcacg gtcgtcctcc cgctgacaag gaaggcccca tgaaggcgaa gatcctttac
      241 atcgaggaca acacccagaa tttctacctc gtcaacttca tcctgaccgc ggcgggaagc
      301 gaggtggtct gggcgaagga cggccccgag ggcgtcgaga aggcggcgct gggctgggcc
      361 gacctgatcc tcctcgacat ccagctcccc ggaatggatg gctacgccgt ggcgcaggcc
      421 ctccgcggga atcccgccct ctccagcacc ccgatcgtcg ccctcacgtc ctacgcgatg
      481 gccggcgatc gggagaaggc tctcgccgcc ggctgcaccg gctacatcga gaagccgatc
      541 gacccgggga gcttcgccgc ccaggtcgag cggcacctcc tctcggcgcg gtcggagggc
      601 ggggcctcgt gagccgggtc ctgatcgtcg acgacatcgt cgagaaccgg tacctcctcg
      661 agacggtcct gaaggcgggc ggctacgagg tcgtctcggc cgccaacggg gccgaggcgc
      721 tcgagacggc ccggatcgcc ccgcccgatc tcctcgtcac cgacatcctg atgccggtca
      781 tggacggctt cgagctctgc cgcaggtgga aggccgacga ggggctccgc gggatcccgc
      841 tcgtcttcta caccgcgacg tataccgact cgaaggacga ggccctcgcg cggacgctcg
      901 gggccgaccg tttcctcgtc aagcctctcc cgccggacac gctgctcgag gaggtccgcg
      961 cggcgctcga gacgcctcgc gcggagagcc cggcggcgga cgccggggag gtccttcagg
     1021 agtacagcga agccctgttc cgcaagctgc agaggaaggt cgcccagctg gaagcggagg
     1081 ccgccacgcg aaggagggcc gaggaggagg tccgggcgct gaacgcagag ctggaggaga
     1141 gggtccggac gaggacggcc gagctcgaga cgagcatccg cgagatggag gcgttctcgt
     1201 actccgtctc gcacgacctc cgcgcccctc tccgcgcgat cgacgggtac tccgcgctcc
     1261 tcgagtcgga ggcgtcggcg aacctcgacg cggagggccg cgagctgctc cggaaggtcc
     1321 gggcgagcgc ccggacgatg tcgacgctca tcgacgagct cctcgagttc tcgcggaccg
     1381 gccgctccga gctccgccgc acccgcctcg acatgtgcga gatcgtccgc tccgccttcg
     1441 agcaggtgac gacgcccgcc gagcgggagc gggtggagct gcgcgtcgac gccctcccgc
     1501 ccgccgacgg cgacgcggcg ctcgtccgcc acgtcgtcac gaacctgctc tcgaacgccg
     1561 tgaagttctc gtccggccgc gagcgcgccg tgatcgaggt cgggagccgc cgcgaaggcg
     1621 accgcaccgc ctacttcgtg aaggacaacg gggccgggtt cgacatgagg tacgccgaca
     1681 agctcttcgg cgtcttccag cgcctccaca gcccggccga gttcccgggc gtcggagtcg
     1741 ggctcgcgat cgtccagcgc atcgtgacgc ggcacggcgg gacggtctcc gccaggggcc
     1801 gtgtgggcga gggagccgag ttcacgttct cgctctgacg tgcgcgcgcg ccggccccgg
     1861 cgcccccggc tacgtctcgc cctcgacgac ctccccgaac gtcccgagct cggagcggat
     1921 tgccgcggcg tcgccgacgg cgacgatccg ggcggagccg gtcccgaaga cgcgccccga
     1981 gacgcggcgg acctctccct tcgtcacctc cgcgaggccc gggacgaacc gggcgatctc
     2041 gtcgggggaa aggccgagga cccagagacg ggcgagctcg tcggcgaccc cggccgccgt
     2101 ctggagcgcg atcgcgaggg agcccgcctc gcggcggaag gcccggtcca tctcctcgtc
     2161 cgtggcctcg gtcgttgcca tccgctccat ctcgtagaga acctcgttga gggcggcgcc
     2221 cgtcacctcc gtccggaccg ccgcgaagga gcggaccgtg ccgcaccctt cgagccagct
     2281 cgccgcagcg ccgggcgagt aggtgtagcc cttgtcctcg cgcaggttct ggacgaggcg
     2341 cgacgtgaag gagccgccgt agatcgtcat cgcgagctcg agggcccggg cgtcccggtc
     2401 ggtcctcggg aagccgaggt tcccgaccat gaggttcgtc tggacggagc cggggcggtc
     2461 gaggacgagg atgcgccgcg gaccgcccgt gggagccggc gccgcgacgg gcggcggcgc
     2521 cgagccgccc gcggcccagt cgccgaacgc gttcccggcc gccgcgatcg cctcctccgg
     2581 cttcacgtcc cccgccacga ggaggagcgc gcgttcgggc cggagacggc gccgggcctc
     2641 ggccgcgagg agcccggggg tgacggcgtc gatcgacgcc gccgtcggcg cgatcgtcgc
     2701 gtacgggtgc gtgccgtaga cctcgcgcgc gaacgcccgg acggccagga acgacggctc
     2761 cgactcgctc gtctcgagct cctcgtgcgc gaggtgccgc gcgcgcgcga ccccgtcggc
     2821 cgggaaggcg gcgcggcggg cgacgtcggc gacgaggtcg aggaggagcg gcaggcgcgc
     2881 ggcgagaccg taggcgccga tcgtgagcga atcgggcccc gcgccggcgg agaggtctcc
     2941 cccggcctcc tgcgccaggt ccgcgaggtc ggccccgctg cgcgacgccg ttccctcgcg
     3001 gagagccgct gcgaggaggc gcgcgagccc gggcctcccg gacgggtcgt ccgagaggcc
     3061 gccgcgcacg acgaggcggg cgctcgcgag cgggacccct cgccacggga cgacctggac
     3121 cgcgaggccg ttcggcaggc gggaagcggc gacctccggc atcgggacgg gacgctcctc
     3181 tccgaggggc gggaggccgt cgggcgcgtt cacgcctccc ccttcggcgg gaccttcacg
     3241 atcgccgccc gccgctccgg cgcgagccac gccgccgcgc gggagaggtc gcccggagag
     3301 accgcctcga cgaggcgggg gaggtcgagg agcgcctcgg gcgcgccggc gatcgccgcc
     3361 gcctgggcga gctcgatcgc gaggccgagg cggggctcga gccccgccca ggtgtcggcg
     3421 aggaccttcg tcttcacgcg cgcgagctcc gcctccccga ccccgtcgcg ggcgatgcgc
     3481 gcgacctcct cgtcgaaagc ggcgaggacc gcttccgccg agacctcgcg ccggtagccg
     3541 atccgggaga ggaagagggt cgtgccgtcg taccagacgc ccccctggaa cgggttgaag
     3601 ccgccggaga ggtccgtcgc gatctcgcgc ccttcgacga ggccgcggta gaggcgcgag
     3661 gcgcgcccgt tgtgaaggag ctcgccgagg acgaggagcc ccgctgcgtc ggcgtgggtc
     3721 cgctccggca tccgccaccc catcgcgacc gccggcgtcc tcgcgagagg cgcctcctgg
     3781 acgaggagga cgggcgcctc gcgcggcggc tcgtgaaggt cgggccgcgc gtacggcgcg
     3841 ccgcgcggga tcgctccgaa gcggcgttcg gcgagcgaga agacctcgtc ggcccccacg
     3901 tctccggcga ccgcgaggac ggcgttgtcg ggccggtaga acgcggagaa gaagccctcc
     3961 acgtcggcga gcgttgccgc gtcgaggtcg tggaaatcgc cgtatccgtc gtgcgcgttc
     4021 tcccacttcg tgaagagcgc ctgcgggagc tcgccgtact cgaagaggcc gtacgggtcg
     4081 ttccggacgt tgacgaggat ctcctccttg acgacgtccc gctggttgtc gagcgactcc
     4141 tggccgaacg ccaggcccga catccggtcg gcctcgagga acaggatccg ctcgagcgcg
     4201 gacgacgggg cgtcgacgac gtagagcgtg acgtccgggc gcgtctgccc gttgttgctg
     4261 ccgcccgagg cctcgcagac cctgtcgaag acccctttcg gggcgttcgg cgtcccctcg
     4321 aacatgaggt gctcgaagag gtgggcgaag cccgagcgcc cgcgcggctc gaggcgcgag
     4381 ccgatccggt agaggacgca gagcccgaag accggcgccg accggttctc cgagacgacg
     4441 accgtcaggc cgttcgggag gacgagggtc tcgaccggga cccgcagcgt caggccttca
     4501 ggagctgccg cgcgatgacc atccgctgga tctcggaggt cccctcgccg atcgtgcaga
     4561 gcttgacgtc gcggtagaac ttctcggcct tgaagtcctt cgtgaagccg tagccgccgt
     4621 ggacctggac ggcgaggttc gcgacgcgga cggagacctc gcccgcgtag agcttgcaca
     4681 tcgccgactc cttcgtgacc ttcatcccgc ggtccttcat ccacccggcc cgccaggtga
     4741 ggaggcgcgc ggcgtcgatc tcggtcgcca tgtcggcgag gtagttctgg atcgactgga
     4801 actcggagat cggcttgccg aactgcacgc gctggcgcga gtaggcgagc gcgcagtcgt
     4861 aggctccctg cgccatcccg agggcgagcg cggcgatcga gatccggccg ccgtcgagga
     4921 cctgcatcgc ctgagtgaag cccgagccga gcggcccgac gagggcctcc ttcgggacgc
     4981 ggcagtcctc catgacgagg gtcgccgtgt cggaggcgcg caggccgagc ttgttctcct
     5041 tcttccccgc gcggatcccc ttctggtgga ggtcgacgac gaaagcggag atgccgtgcg
     5101 tcccggcggc gcggtccgtc acggccatca ggaccgcgac gtcgccgacg gagccgtgcg
     5161 tgatgaagtt cttcgagccg ttgaggaccc actcgttccc gtcgaggacg gcggtcgtcc
     5221 gggtcccctt cgcgtcgctc cccgcctcgg cctccgtgag gccccaggcg ccgatcatc
//
LOCUS       DAOTEZ010000313         5242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_11397_length_5242_cov_7.289184, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000313 DAOTEZ010000000
VERSION     DAOTEZ010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11397_length_5242_cov_7.289184"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="P5164_14650"
     CDS             complement(<1..248)
                     /locus_tag="P5164_14650"
                     /inference="COORDINATES: protein motif:HMM:NF021462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HRY45182.1"
                     /translation="MTTSTLARPVRPAAAALVATAATFLALDLAWLGVVARDLYARTL
                     GDLLRPDAYLPAAALFYTFYVAVTVAWAVLGARSKGDAA"
     gene            329..1684
                     /locus_tag="P5164_14655"
     CDS             329..1684
                     /locus_tag="P5164_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45183.1"
                     /translation="MDGRIARVRKGGRPGPGRGRGRRALGPVLAILLPALAEGAAPRP
                     AVVRESWRKVEAGPVTVVGTASDPRLVETAATLGTLVALLPGIVPGAFGSRPPLVVAL
                     AHEPEHLSRISLPSREGGRIVLAAAGGEEAREALVRAAVVALARRSRPALPEWAATGL
                     AEYLSTASVSGARATLGRLVPQHVSLLRAAGPPAGRPALFVAPSLAGDGPAAALLRAE
                     AWAAVHYLLHALPDGGERLARHAALLGEGRDAESAFAEAFGETERSLLLRARAHAVAG
                     RSLARFLPVASAPPGRVLPLTRTGAAGLLGGAVARGRPAEEPAASLPEPPAPSSPAAR
                     RPSPTLSRDVPAEVDLVNRLIDAGREEEALARLEALHASLGRDPEMQRALGWDVAEVR
                     RVVVHNRLVRRYNDAIALLNAGRLAEALPIFREVAEKAEDPGLRRLAWERATAPDGGR
                     R"
     gene            1779..2858
                     /locus_tag="P5164_14660"
     CDS             1779..2858
                     /locus_tag="P5164_14660"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRY45184.1"
                     /translation="MTERDGTTTGGVERRDLLKLGAAAAAGLVAGRLSAEPATAEAPK
                     PAATGPAGHPWNPRTADAMPTRNLGKTGFRVGIFSLGGQAAIEKAENDAVAVPLIERA
                     LDLGVNYVDTSARYGGEARWSERYFGQVLPKRRSEVFVATKTHDRGRDGSLKLLETSL
                     KLLRTDHVDLWQLHAMSTMDDVEKVTARGGALEAFREAREQKLVRFLGLSGHTDPAVL
                     AEAIRRFPFDTVLLAVNAADPHHLPFASELLPLAVEKEMGIVGMKIPARGRLLAGYVP
                     PPLEEQRGRAKATRTGTLSMAEAMRYVLTLPVSTVIVGCDSIAQLEENVAIARAFTPL
                     SGAQLAALEAKAAPVAQQSLWFRRA"
     gene            2901..4556
                     /locus_tag="P5164_14665"
     CDS             2901..4556
                     /locus_tag="P5164_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HRY45185.1"
                     /translation="MLLPGAPAALVLALVAGLGLALGSVKVRGVGLTTAGVLFVGLLL
                     GHFGVAIEPEVLEFARDFGLILFVTSIGLQVGPGFVSSLRRSGMTLNLLATAVVLLGV
                     GLAAAAHLLLGVELPAVVGILSGATTNTPSLAAAQQALKEVATDTTSLARGATIPPLG
                     YAVAYPFGVAGIILAILVLKAAFRVSMPLEEKLLERSRRSEAPPLHRANLEVTNTNLD
                     GLLLEKLPLVDRAGLVVSRVYHGGELLVPTHETRLAVGDVLLAVGPKKDLDDLRLLVG
                     KESDLDLRSLPTPITSKRLIVTRKEVVGKTLGELSFAARFGVRITRVSRSELEMAAYP
                     GFELRTGDAILAVGGPEEIRKVAAELGDSPRDLNYPHLVPVFLTIALGVAVGLFPIHL
                     QGMPAPVKLGLAGGPLLVAILLSRLGRVGPLVFWLPISANFMLREIGIALFLAAVGLR
                     SGAGFVDTLVKGPGLTWMAIGAVITIVPMLVVGAIGRLVFRVNFLTLTGVMAGSMTDP
                     PALAFANGLAGSDAPTLGYVAVYPLTMILRIVSAEVLVLLLAR"
     gene            4605..>5242
                     /locus_tag="P5164_14670"
     CDS             4605..>5242
                     /locus_tag="P5164_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HRY45186.1"
                     /translation="MSSRARAALFFALAAASLLPWVGPAAALAGGIVFSLLFGNPAPT
                     RTASWSKVLLQASVVGLGFGLSLGAVARVGAASFLYTAVGIALTLALGVLLGRLAGTS
                     GPVSTLISFGTAICGGSAIAAMAPVVKAKSDEIAVSLATVFTLNAAALLVFPAVGHLV
                     GLDPARFGLWAALAIHDTSSVVGAASAYGPEALALATTVKLTRALWIVPCVLG"
BASE COUNT          588 a         2020 c         1898 g          736 t
ORIGIN      
        1 gcggcgtccc ccttcgaccg ggcgccgagg acggcccagg cgacggtgac ggcgacgtag
       61 aacgtgtaga agagcgcggc cgccgggagg taggcgtcgg ggcggaggag gtctcccagc
      121 gtccgcgcgt agaggtcgcg agccacgacg ccgagccacg cgaggtcgag ggcgaggaac
      181 gtcgcggccg tcgcgacgag cgcggcggcg gccggtctca cggggcgggc gagcgtcgag
      241 gtcgtcatcc gccccctcta cgccgggcgg ccccgatcgg acgtggccgg cgcacgggat
      301 ggcggccccg ggcttcatac ttgccgccgt ggacggacga atcgcacgcg tgcggaaggg
      361 cgggaggccg ggacccgggc ggggccgggg gcgccgcgcg ctcgggccgg tcctcgcgat
      421 cctcctgccg gcgctcgcgg agggcgcggc gccccggccc gccgtcgtcc gggagtcgtg
      481 gcggaaggtg gaggcggggc cggtcacggt ggtcgggacc gcctccgacc cgcggctagt
      541 cgagacggcg gcaacgctcg ggacgctcgt cgcgctcctc cccgggatcg ttcccggggc
      601 cttcggctcg cgcccgccgc tcgtcgtcgc tctggcgcac gaaccggagc acctctcccg
      661 gatctccctg ccgtcccgcg agggagggcg gatcgtcctc gcggccgcag gcggcgaaga
      721 ggcgcgcgag gcgctcgttc gtgccgcggt cgtcgcgctc gcgcgacgct cgcgtcccgc
      781 gctcccggag tgggccgcga cggggctcgc cgagtacctt tcgaccgcct ccgtctcggg
      841 cgctcgcgcc acgctcggcc gcctcgtccc gcagcacgtc tcgctcctcc gcgcggccgg
      901 gccgccggcc ggccggccgg cgctcttcgt cgccccgtcg ctcgcgggcg acggaccggc
      961 tgccgccctc ctccgtgccg aggcgtgggc cgccgtccac taccttctcc acgccttgcc
     1021 ggacggcgga gagcgcctcg cccgccatgc ggcgctcctc ggggaggggc gcgacgccga
     1081 gtcggcgttc gcggaggcgt tcggtgagac ggagcggagc ctcctcctcc gtgcgcgcgc
     1141 ccacgccgtc gccggccgct ctctcgctcg cttccttccg gtcgcttcgg cgccgccggg
     1201 gcgtgtcctg cctttgacgc gcacgggcgc cgcggggctc ctcggcgggg ccgtcgcgag
     1261 ggggcgcccc gcggaggagc cggccgcgag cctcccggag ccgcccgctc cgtcctcccc
     1321 cgcggcgcgg cgcccgtccc cgaccctctc ccgcgacgtg ccggccgagg tcgacctcgt
     1381 gaaccggctc atcgacgcgg ggcgcgagga ggaggcgctc gcgcgtctcg aggcgctcca
     1441 cgcctccctc gggcgcgacc cggagatgca gcgcgcgctc ggctgggacg tcgcggaggt
     1501 gcggcgcgtc gtcgtccaca accggctcgt ccgccgctac aacgacgcga tcgcgctcct
     1561 gaatgccggg cgtctcgccg aggcgctccc gatcttccgc gaggtcgcgg agaaggccga
     1621 ggaccccggc ctccgccgcc tcgcgtggga gagggcgacc gcgccggacg gcgggaggcg
     1681 ctgagcgtcg aggggagggc gctcctcggg acccctcgat gcgcgtccgc gagacgcgcc
     1741 atacttcagc cgccgggacc ccggcgggag gtgcggtgat gacggagcgc gacgggacga
     1801 cgacgggcgg cgtggaacgg cgcgacctcc tgaagctcgg ggcggccgcc gccgcggggc
     1861 tcgtcgcggg gcgcctctcg gccgagccgg cgacggccga ggcgccgaag cccgccgcga
     1921 cgggacctgc gggccatccc tggaacccga ggaccgccga cgcgatgccg acgcgcaacc
     1981 tcgggaagac gggcttccgg gtcggcattt tcagcctcgg cgggcaggcg gcgatcgaga
     2041 aggcggagaa cgacgcggtc gccgttcccc tcatcgagcg ggccctcgac ctcggcgtga
     2101 actacgtcga cacctccgcc cgctacggcg gcgaggcgcg ctggagcgag cgctacttcg
     2161 ggcaggtgct gccgaagagg cggagcgagg tcttcgtcgc gaccaagacg cacgaccgcg
     2221 ggcgcgacgg ctccctgaag ctcctcgaga cgtcgctgaa gctccttcgg acggaccacg
     2281 tcgacctctg gcagctccac gcgatgtcga cgatggacga cgtcgagaag gtgacggccc
     2341 ggggaggcgc cctcgaggcg ttccgcgagg cgcgcgagca gaagctcgtc cgcttcctcg
     2401 gcctgtcggg ccacaccgac ccggccgtcc tcgccgaggc gatccgccgc ttcccgttcg
     2461 acaccgtcct cctcgcggtc aacgccgccg acccgcacca cctcccgttc gcgagcgagc
     2521 tcctcccgct cgccgtggag aaggagatgg ggatcgtcgg gatgaagatc ccggcgcggg
     2581 ggcggctcct cgccggatac gtcccgccgc cgctcgagga gcagcgcggg cgcgcgaagg
     2641 cgacgcggac ggggaccctc tcgatggccg aggcgatgcg ctacgtcctg acgctccccg
     2701 tctcgaccgt catcgtcggc tgcgactcga tcgcccagct cgaggagaac gtcgcgatcg
     2761 cgagggcctt caccccgctc tcgggggcgc agctcgccgc cctcgaggcg aaggccgccc
     2821 cggtcgcgca gcagtcgctc tggttccgaa gggcgtagcg ccacgtagac gggccgcacg
     2881 gaccgcgcga gaatccgccg atgctccttc ccggggcccc ggcggccctc gtcctcgccc
     2941 tcgtcgccgg cctcgggctc gccctcggct cggtgaaggt gcggggcgtc ggcctgacga
     3001 cggcgggggt cctcttcgtc ggcctcctcc tcgggcactt cggcgtcgcg atcgagccgg
     3061 aggtgctgga gttcgcgcgg gacttcggcc tcatcctctt cgtcacgtcg atcggcctgc
     3121 aggtcgggcc cggcttcgtc tcctcgctcc ggcgaagcgg gatgacgctg aacctcctgg
     3181 cgacggcggt cgtcctcctc ggcgtcggcc tcgccgccgc ggcgcacctc ctcctcggcg
     3241 tcgagctccc ggccgtcgtc ggcatcctct cgggcgcgac gacgaacact ccgagcctcg
     3301 ccgcggccca gcaggcgctg aaagaggtcg cgaccgacac gacgtccctc gctcgcggag
     3361 cgacgatccc tccgctcggc tacgcggtcg cctacccgtt cggcgtcgcc ggaatcatcc
     3421 tcgcgatcct cgtcctgaag gccgcgttcc gcgtctcgat gccgctcgag gagaagctgc
     3481 tcgagcgctc ccgccggagc gaggcgccgc cgctccaccg cgcgaacctc gaggtgacga
     3541 acacgaacct ggacggcctc ctgctcgaga agctccccct cgtcgaccgg gccggactcg
     3601 tcgtctcgcg cgtctaccac ggcggcgagc tcctcgtccc gacgcacgag acgcgcctcg
     3661 ccgtcggcga cgtcctcctc gcggtcgggc caaagaagga cctcgacgac ctgcggctcc
     3721 tcgtcgggaa ggagagcgac ctcgaccttc ggagcctccc gacgccgatc acgagcaagc
     3781 ggctcatcgt cacgcgcaag gaggtcgtcg ggaagacgct cggcgagctg agcttcgcgg
     3841 cccgcttcgg cgtgaggatc acccgcgtct cgcgctccga gctggagatg gccgcgtacc
     3901 ccggcttcga gctccggacg ggcgacgcga tcctcgccgt cggcggcccg gaggagatcc
     3961 ggaaggtcgc cgccgagctc ggcgactcgc cccgcgacct gaactacccg cacctcgtcc
     4021 cggtcttcct gacgatcgcg ctcggcgtcg ccgtcggcct cttcccgatc cacctgcagg
     4081 ggatgccggc gccggtgaag ctcggcctcg cgggcgggcc gctcctcgtc gcgatcctcc
     4141 tctcgcggct cgggcgcgtc gggccgctcg tcttctggct ccccatcagc gcgaacttca
     4201 tgctccgcga gatcggcatc gcgctcttcc tcgcggcggt cggcctccgc tccggcgcgg
     4261 gattcgtcga cacgctcgtg aaggggccgg ggctgacctg gatggcgatc ggcgcggtga
     4321 tcacgatcgt cccgatgctc gtcgtcggcg cgatcgggcg gctcgtcttc cgcgtcaact
     4381 tcctgacgct gacgggcgtg atggccggga gcatgacgga cccgccggcg ctcgcgttcg
     4441 cgaacggcct cgccggctcg gacgcgccga cgctcggcta cgtcgccgtc tacccgctca
     4501 cgatgatcct ccggatcgtc tcggcggagg tcctcgtcct cctcctcgcg cgctgaagcg
     4561 ccaccaccgc ttgcggcggg ccgcgcggct cgaataatgc tcgggtgtcc tcgcgcgccc
     4621 gcgccgccct cttcttcgcc ctcgccgccg cgagccttct cccatgggtc ggcccggccg
     4681 cggccctcgc gggtggcatc gtcttcagcc tcctcttcgg gaaccccgct ccgacccgga
     4741 cggcctcgtg gagcaaggtc ctcctccagg cctcggtcgt cggcctcggc ttcgggctga
     4801 gcctcggggc ggtcgcccgc gtcggcgccg cgtctttcct ctacacggcg gtcgggatcg
     4861 cgctgacgct cgcgctaggg gtcctcctcg gccgcctcgc cgggacctcg gggccggtct
     4921 cgacgctgat ctcgttcggg acggcgatct gcggcgggag cgcgatcgcc gcgatggccc
     4981 cggtcgtcaa ggcgaagagc gacgagatcg ccgtctcgct cgcgacggtc ttcacgctga
     5041 acgcggcggc tctcctcgtc ttcccggccg tcgggcacct cgtcggcctc gacccggcgc
     5101 ggttcggcct ctgggcggcg ctcgcgatcc acgacacgtc gagcgtcgtc ggcgccgcct
     5161 cggcgtacgg accggaagcg ctggcgctcg ccacgacggt gaagctgacg cgggcgctct
     5221 ggatcgtgcc gtgcgtcctc gg
//
LOCUS       DAOTEZ010000314         5238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_11411_length_5238_cov_5.091067, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000314 DAOTEZ010000000
VERSION     DAOTEZ010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11411_length_5238_cov_5.091067"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /gene="hisH"
                     /locus_tag="P5164_14675"
     CDS             <1..399
                     /gene="hisH"
                     /locus_tag="P5164_14675"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRY45187.1"
                     /translation="GARLLGLCLGFQLLFEASEEFGTTEGLGLLPGRVVPFPSSVRVP
                     HVGWNRLETEGAAEGPLLAGLPRGTYVYFVHSFRPEGADPADVAAFCDHGGRFVAAAR
                     RGNVWGCQFHPEKSSAAGRRILANFLAEAA"
     gene            396..1142
                     /locus_tag="P5164_14680"
     CDS             396..1142
                     /locus_tag="P5164_14680"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HRY45188.1"
                     /translation="MIDLWPAIDLMGGKVVRLLHGDPSQRTVYPERAVEVAVRFAREG
                     ADGIHVVDLDAAFGTGSNAPSIAEIVAASTVPVEVGGGLRDGAGVEAALASGVSRVVL
                     GSLPFRDPALFAALLAEFAERLVVALDCREGRPTVHGWVEDAGAGDAATVAARLAAEG
                     VRSLLVTDVARDGAMTGPNLDLLAAVRADFPGELLASGGMRGEEDLAPVAAALAGGPA
                     GAIFGRALHGGATTVARLAAARAALGEGTR"
     gene            1139..1891
                     /gene="hisF"
                     /locus_tag="P5164_14685"
                     /pseudo
     CDS             1139..1891
                     /gene="hisF"
                     /locus_tag="P5164_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005111203.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
     gene            1865..2593
                     /gene="hisIE"
                     /locus_tag="P5164_14690"
     CDS             1865..2593
                     /gene="hisIE"
                     /locus_tag="P5164_14690"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF002747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HRY45189.1"
                     /translation="MAKAGVPVRPAIPAGLEEVAYDERGLVPVVVRNAGTGEVLTLAW
                     ANDEALALTVETRQTHLFSRSRRSLWKKGETSGNVQRVVRVFLDCDRDAVLYDVEPSG
                     PACHTGAASCFSPISAIPSDAAARVALPATVNGASAEASGADPFGLGPLFDVVAARKA
                     RPEPGSYTNRLLEKGIGKCAQKVGEEGVEVALAAVSRDDAGLAAEVADLMYHVVVLMA
                     ARGLSPEAVAAELAARRGVRREEK"
     gene            2593..3984
                     /locus_tag="P5164_14695"
     CDS             2593..3984
                     /locus_tag="P5164_14695"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HRY45190.1"
                     /translation="MAKNEARTLVVLERPADLTTPVAVMRTLLSDDEPCFLLESVEGG
                     ERVARWSFLGTAPERSFSGAEGDPFTLLRGVVRSETRALPASDGFGDDLPPFIGGAVG
                     FAGYDAVRRLERLPSTNPDVVGLPDAWFGVFETVLAFDHVRHRLLFLGNARPGEEAAV
                     RTRLATLAARVDAGRPHGGRPARLAGPWEETLPRKAYVDRVERAKEHIRAGDVFQVVL
                     SRRWSAPIEGDPFSIYRALRRVSPSPYQYYLRTPDAVIFGASPERLVRAGDDDVETIP
                     LAGTRRRGATPEEDLALARELLADEKERAEHVMLVDLGRNDLGRVAAPGTVKVRDFFT
                     VERFSHVMHLATRVTGRLAEGKDAVDALGATFPAGTLTGAPKIRAMEVIEELEPVRRG
                     AYGGAVGYLDFAGHLDMAIGIRTAVVKDGVLHVQAGAGIVADSRPEAEADECESKAWA
                     LMRAVDAAKEYEP"
     gene            3981..4550
                     /locus_tag="P5164_14700"
     CDS             3981..4550
                     /locus_tag="P5164_14700"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012408079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRY45191.1"
                     /translation="MILVVDNYDSFTFNLVQVLQTLHPEVRVVRNDAMTATEALALKP
                     ARIVISPGPGRPEDAGISEELIRRNEEVPLLGVCLGHQAIGHVFGASIARAPVLMHGK
                     TSEIRHDGEGIYAGLGNPFVATRYHSLVVDRPTVPHCLAITAETSDGVVMGLRHRSLP
                     LVGVQFHPESILTLEGERLLRNFVEKGAS"
     gene            4547..>5238
                     /gene="trpD"
                     /locus_tag="P5164_14705"
     CDS             4547..>5238
                     /gene="trpD"
                     /locus_tag="P5164_14705"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRY45192.1"
                     /translation="MSLPAPGAGDLHRALKLVMEGRTLSREEARLAVDEMLGESFSQV
                     RAAALLAAMARRGEGPEEVAGVVDALRSRAARPPVAEELAARSVDVCGTGGDGQGTFN
                     VSTTAAFVVAGAGVPVAKHGNRAVSSASGSADVLSALGVRIDMPPVVAARALAEANVT
                     FLFAPAYHAAMRSVGPMRRELGIRTAFNLAGPLANPLGVTRQLVGVDRPLRVPVLAHA
                     LAALGAAHALVVS"
BASE COUNT          609 a         1962 c         1961 g          706 t
ORIGIN      
        1 ggcgcgcgcc tcctcggcct ctgcctcggc ttccagctcc tcttcgaggc gagcgaggag
       61 ttcgggacga ccgagggcct cggcctcctc ccggggcgcg tcgtcccctt cccttcgagc
      121 gtccgcgtcc cgcacgtcgg atggaaccgg ctcgagaccg agggcgcggc ggaggggccg
      181 ctcctcgcgg gactgccccg cgggacgtac gtctacttcg tccactcgtt ccgccccgag
      241 ggggccgacc cggccgacgt cgcggccttc tgcgaccacg gcggccgctt cgtcgccgcg
      301 gcccgccgcg gcaacgtctg gggctgccag ttccaccccg agaagtcgtc agccgccggg
      361 cggcggatcc tcgcgaactt cctcgcggag gccgcgtgat cgacctctgg cccgcgatcg
      421 acctgatggg cgggaaggtc gtccgcctcc tccacggcga cccgtcgcag aggacggtct
      481 acccggagcg cgccgtcgag gtcgccgtcc gcttcgcgag ggaaggggcc gacgggatcc
      541 acgtcgtcga cctcgacgcc gccttcggca cggggtcgaa tgctccttcg atcgcggaga
      601 tcgtcgcggc ctcgaccgtc cccgtcgagg tcggcggcgg cctgcgcgac ggcgcgggcg
      661 tcgaggcggc gctcgcctcg ggcgtctccc gcgtcgtcct cgggagcctc ccgttccgcg
      721 acccggcgct cttcgcagct ctccttgccg agttcgccga gcgcctcgtc gtcgccctcg
      781 actgccggga ggggcggccg acggtccacg gctgggtgga ggacgccggg gcgggagacg
      841 cggccacggt cgcggcccgc ctcgcggcgg agggggtccg gtctctcctc gtcaccgacg
      901 tcgcgcgcga cggcgcgatg acgggcccga acctcgacct cctcgccgcg gtccgggccg
      961 acttcccggg cgagctcctc gcctcgggcg ggatgcgcgg cgaggaggac ctcgccccgg
     1021 tcgccgccgc cctcgcgggc ggccccgcgg gggcgatctt cggccgggcg ctgcacggcg
     1081 gggcgacgac ggtcgcccgg ctcgctgcgg cccgcgccgc gctcggggag ggaacgcgat
     1141 gagcctcgcc gcgagggtga tcccgtgcct cgacgtggcg gaaggacgcg tcgtcaaggg
     1201 gctgaggttc gagaacctgc gcgacgcggg agaccccgtc gaggccgcgc gccgctacga
     1261 ggccgagggg gccgacgagc tctgcttcct cgacgtcgcc gcctcgcacg aggggcgcgg
     1321 gacgctcgtg gacctcgtcc gccgcgtcgc cgacgtcctc gcgatcccct tcaccgtcgg
     1381 cggcggcgtc cgctccgtcg aggacgccgg cgtgctcctc tcggcggggg ccgaccgcgt
     1441 taccgtgaac accgcggccg tcctcgaccc cgcgctcgtg acgcgcctcg cggagcgctt
     1501 cggcgcgcag tgcgtcgtcg tcgccgtcga cgcgaagcgg gcgggcgagg gcttcgtcgt
     1561 ctcgacgcac ggcgggcgga ggctgaccga gatcgggctt cccgcctggg tcgccgaggt
     1621 gacggcccgc ggggcggggg agatcctcct cacctcgatg gacgccgacg ggacgaaggc
     1681 gggcttcgac gtcgcgatgc tgaaggcggc ccgcgcggcc acgacgcttc cgatcgtggc
     1741 gtcgggcggc gcgggggagc tcgcgcactt cgcgccggcg gtcctcgagg ggggcgccga
     1801 cgccgtcctc gcggcgtcgg tcttccacga ccgggtcttc agcgtcggaa tggtgaagcg
     1861 ggcgatggcg aaggcgggcg tcccggtccg ccccgcgatc cccgcgggcc tcgaggaggt
     1921 cgcgtacgac gagcgcggcc tcgtccccgt cgtcgtgagg aacgccggca cgggcgaggt
     1981 gctgacgctc gcctgggcga acgacgaggc cctcgcgctc accgtcgaga cgcggcagac
     2041 gcacctcttc agccgctcgc gccgctcgct ctggaagaag ggggagacct cggggaacgt
     2101 ccagcgcgtc gtccgcgtct tcctcgactg cgaccgcgac gccgtcctct acgacgtcga
     2161 gccatcgggg ccggcctgcc acaccggggc cgcgagctgc ttctcgccga tatcggcgat
     2221 cccgtcggac gcggcggcgc gggtcgccct ccccgcgacg gtgaacggcg cgagcgccga
     2281 ggcgtccggc gccgacccgt tcggcctggg tcccctcttc gacgtcgtgg cggcgcggaa
     2341 ggcgcgtccc gagccgggct cgtacacgaa ccgtcttctc gagaagggga tcgggaagtg
     2401 cgcgcagaag gtcggggagg agggggtcga ggtggccctc gcggcggtct cgcgcgacga
     2461 cgcgggactc gcggcggaag tcgccgacct gatgtatcac gtcgtcgtcc tgatggccgc
     2521 ccgggggctc tcccccgagg cggtcgcggc ggagctggcg gcacggcgcg gggtgcgccg
     2581 ggaggagaag tgatggcgaa gaacgaagcc cggacgctcg tcgtcctcga gaggccggcc
     2641 gacctgacga cgccggtcgc cgtgatgcgg acgctcctct cggacgacga gccctgcttc
     2701 ctcctcgagt cggtcgaggg cggagagcgc gtcgcacgct ggtcgttcct cgggactgcc
     2761 ccggagcgct ccttcagcgg cgccgagggc gacccgttca ccctcctccg cggcgtcgtc
     2821 cgctcggaga cgcgcgcgct ccccgcctcc gacggcttcg gcgacgacct gccgccgttc
     2881 atcggcgggg cggtcggctt cgccgggtac gacgcggtgc ggcggctcga gcgcctcccg
     2941 tcgacgaacc ccgacgtcgt cggcctgccg gacgcctggt tcggggtctt cgagacggtc
     3001 ctcgcgttcg accacgtgag gcatcgtctc ctcttcctcg gcaacgcccg gcccggcgag
     3061 gaggcggccg tccgcacccg gctcgcaacg ctcgccgcgc gcgtcgacgc cggccggcct
     3121 cacggcgggc ggcccgcgcg cctcgcgggc ccgtgggagg agacgctccc ccggaaggcc
     3181 tacgtggacc gcgtcgagcg ggcgaaggag cacatccgcg cgggcgacgt cttccaggtc
     3241 gtcctctcgc gccgctggtc ggcgccgatc gagggggacc ccttctcgat ctaccgcgcc
     3301 ctgcggcgcg tcagcccgtc gccctaccag tactacctcc ggacccccga cgccgtgatc
     3361 ttcggagcct ccccggagag gctcgtccgc gcgggggacg acgacgtcga gacgatcccg
     3421 ctcgccggaa cgcgccggcg cggcgcgacc ccggaggagg acctcgccct cgcgcgcgag
     3481 ctgctcgccg acgagaagga gcgagccgag cacgtgatgc tcgtcgacct gggccggaac
     3541 gacctcggcc gggtcgccgc gccggggacg gtgaaggtcc gcgacttctt caccgtcgag
     3601 cgcttctcgc acgtcatgca cctcgcgacg cgcgtcaccg gacgcctcgc cgaaggcaag
     3661 gacgcggtcg acgccctcgg cgcgaccttc ccggcgggga cgctcacggg cgccccgaag
     3721 atccgggcga tggaggtcat cgaggagctc gagccggtgc ggcgcggcgc ctacggcggc
     3781 gcggtcggct acctcgactt cgcggggcac ctcgacatgg cgatcggcat ccggacggcg
     3841 gtcgtgaagg acggcgtcct ccacgtccag gccggcgccg gcatcgtcgc cgactcgcgc
     3901 ccggaggccg aggcggacga gtgcgagagc aaggcgtggg cgctcatgcg cgccgtcgac
     3961 gcggcgaagg agtacgagcc gtgatcctcg tcgtcgacaa ctacgactcg ttcaccttca
     4021 acctcgtcca ggtcctccag acgctccacc ccgaggtccg cgtcgtccgg aacgacgcga
     4081 tgacggccac ggaagcgctc gcgctgaagc cggcgcggat cgtgatctcg cccgggccgg
     4141 ggcgccccga ggacgcgggg atcagcgagg agctgatccg gcgcaacgag gaggtcccgc
     4201 tcctcggcgt ctgcctcggg caccaggcga tcgggcacgt cttcggcgcg tccatcgcga
     4261 gggcgcccgt cctgatgcac gggaagactt ccgagatccg ccacgacggg gaggggatct
     4321 acgcgggcct cgggaacccg ttcgtcgcga cgcgctacca ctccctcgtc gtcgaccggc
     4381 cgacggtccc gcactgtctc gcgatcacgg cggagacgtc ggacggggtc gtcatggggc
     4441 ttcgccaccg ctcgctccct ctcgtcggcg tccagttcca ccccgagtcg atcctcacgc
     4501 tggaagggga gcggctcctc cggaacttcg tcgagaaggg cgcgtcgtga gcctccccgc
     4561 gccgggcgcg ggcgacctcc accgcgcgct gaagctcgtc atggaagggc ggacgctctc
     4621 gcgggaggag gcgcgcctcg ccgtcgacga gatgctcggg gagagcttct cgcaggttcg
     4681 cgccgcggcg ctcctcgcgg cgatggcgcg gcgcggggag gggccggagg aggtcgccgg
     4741 cgtcgtcgac gcgcttcgct cccgggcggc ccggccgccc gtcgcggagg agctcgccgc
     4801 gcgctccgtc gacgtctgcg ggacgggcgg cgacggccag gggacgttca acgtctcgac
     4861 gacggcggcg ttcgtcgtcg cgggagcagg cgtcccggtc gccaagcacg gcaaccgggc
     4921 cgtctcttcg gcgagcgggt cggccgacgt cctctccgct ctcggcgtga ggatcgacat
     4981 gccgccggtg gtcgcggcgc gggcgctcgc ggaggcgaac gtcacgttcc tctttgcccc
     5041 ggcgtatcac gcggcgatga gatccgtcgg gccgatgcgc cgagagctcg gcatccgcac
     5101 ggcgttcaac ctcgccggcc cgctcgcgaa cccgctcggc gtgacgcgcc agctcgtcgg
     5161 cgtcgaccgc ccgctccgcg tcccggtcct cgcgcacgcc ctcgcggcgc tcggcgcggc
     5221 gcacgcgctc gtcgtctc
//
LOCUS       DAOTEZ010000315         5230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_11437_length_5230_cov_8.585507, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000315 DAOTEZ010000000
VERSION     DAOTEZ010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11437_length_5230_cov_8.585507"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1632
                     /locus_tag="P5164_14710"
     CDS             <1..1632
                     /locus_tag="P5164_14710"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HRY45193.1"
                     /translation="VLIPLNQEKLPGCYLHRSDPADVARVEHLTFICTKTKEEAGPNN
                     NWMAPAEAYNKLGQLYDGSMKGRTMYVIPYVMGPIGSPQAKVGVELTDSVYVVLNMRI
                     MTRMGTRALRMLGDSADFNRGLHSMLDLNPERRFICHFPEDNTIWSIGSGYGGNVLLG
                     KKCLALRIGSYLGRQEGWMAEHMLIMGVEEPSGETTYVAAAFPSACGKTNFSMLIPPS
                     SFNGWKVWTVGDDICWMRPGPDGRLWAINPEAGYFGVAPGTNYATNPNAMESIKRDTL
                     YTNVAMTPDGDVWWEGKGSTPPEELTDWRGRPWQRGSTEKAAHPNSRFTAPAANNPAL
                     SPRVNDRNGVPISAIIFGGRRSTTVPLVLQSFNWTHGVYLGATMGSETTAAAVGKEGV
                     VRRDPMAMLPFCGYDMGRYFEHWLNMEGKVATLPKIFMVNWFRKGPDGSFLWPGFGEN
                     MRILKWIIDRARGRAGASESAIGWVPKWADLPLKDLAVPMEDVEDAQTIDYEEWKKEL
                     ADQDTFFEQFSSTMPKEIVYEREIIRSRLNRVTAK"
     gene            complement(2558..3505)
                     /gene="pip"
                     /locus_tag="P5164_14715"
     CDS             complement(2558..3505)
                     /gene="pip"
                     /locus_tag="P5164_14715"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015133302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HRY45194.1"
                     /translation="MPDLFPPIEPFDSFRLRVSDLHELHVEQSGSPDGLPVVFFHGGP
                     GAGISPLHRRFFDPAAYRVILFDQRGSGRSTPLGELRENTTWDLVADAERIRERLGIE
                     KWLVFGGSWGSTLGLAYAETHPERCTGVILRGIFLSRRSEVLWTFGGGAQRVFPDGWA
                     EFLSNLDESEKDDVLAAYHRRLNAEDPVVRRQAALAWNRWEELGSYLVAHNYSVAEAD
                     VPAEVALARIEAHYFVNGAFLSDDEQLLRDVEKVKHLPCVIVQGRYDMVCPAVSAWDL
                     HQAWPGSRLVIVPASGHSADEPGIVSALVDATEEWKKRG"
     gene            3594..>5230
                     /gene="speA"
                     /locus_tag="P5164_14720"
     CDS             3594..>5230
                     /gene="speA"
                     /locus_tag="P5164_14720"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HRY45195.1"
                     /translation="MPTATARKSSARSAKKGPRTPDRFAEAEELYGIDAWGKGFFSIS
                     DDGHLLVHPTREGHRFADLKDVVDEVGGRGITPPMIIRFPQILASSVRELNEAFGKAI
                     KEFGYDGDYRGVFPIKVNQKKVVVHEIIEAGRKYGYGLEAGSKPELIAALSQDLGPEC
                     LITTNGYKDEAFIRLALDGVKMGRNVILTLEKVSELERVLEVAKKRGVKPLVGMRAKL
                     YARGSGKWAKSGGEAAKFGLTTTEMLAAIEILKSKRMLDSLVMLHFHIGSQITDIRKI
                     KDAIREAGRVYAKLSRSGVEVRYLNLGGGLGVDYDGSKTSFDSSMNYTVQEYANDVVY
                     NIKTICEDEKVPVPTLVTESGRAVTAYHSVLVSNVLDVADKIEQDKTVTLNGDENHVV
                     RELWDVFKGVTAKNVRESYHDALQYKEELFTLFNLGYVSLEDRSKGEILFWDVCDKIR
                     RHLKGLREIPEEFEDLERELADKYVVNFSVFQSVPDSWAIDQLFPILPIHRLREKPGE
                     IATLADITCDSDGKIEKFIDLRDIKEFLPLHSWKRSQP"
BASE COUNT          908 a         1811 c         1795 g          716 t
ORIGIN      
        1 gtcctgatcc cgctcaacca ggagaagctc cccggctgct acctccaccg ctccgacccg
       61 gccgacgtcg cgcgggtcga gcacctcacc ttcatctgca cgaagacgaa ggaggaggcg
      121 gggccgaaca acaactggat ggcgccggcc gaggcctaca acaagctcgg ccagctctac
      181 gacggctcga tgaaggggcg gacgatgtac gtcatcccct acgtcatggg cccgatcggc
      241 tcgccgcagg ccaaggtggg cgtcgagctg accgacagcg tctacgtcgt cctgaacatg
      301 cggatcatga cgcgcatggg gacgcgcgcc ctccggatgc tcggcgactc ggccgacttc
      361 aaccgcggcc tccactcgat gctcgacctg aaccccgagc ggcgcttcat ctgccacttc
      421 cccgaggaca acacgatctg gtcgatcggc tcgggctacg gcgggaacgt cctgctcggc
      481 aagaagtgcc tcgccctgcg catcggctcc tacctcggcc gccaggaagg ctggatggcc
      541 gagcacatgc tcatcatggg ggtcgaggag ccgagcggcg agacgacgta cgtcgccgcg
      601 gcgttcccgt cggcctgcgg caagacgaac ttctcgatgc tcatcccgcc gtcgagcttc
      661 aacggctgga aggtctggac cgtcggcgac gacatctgct ggatgcgccc cgggccggac
      721 ggccggctct gggcgatcaa ccccgaggcg ggctacttcg gcgtcgcgcc cgggacgaac
      781 tacgccacga acccgaacgc gatggagtcg atcaagcgcg acacgctcta cacgaacgtc
      841 gcgatgacgc cggacggaga cgtctggtgg gaggggaagg gcagcacgcc gccggaggag
      901 ctgacggact ggcgcggccg gccgtggcag cgcggctcga cggagaaggc ggcccacccg
      961 aacagccgct tcaccgcccc ggccgcgaac aaccccgcgc tctcgccgcg cgtgaacgac
     1021 cggaacggcg taccgatctc cgcgatcatc ttcggcggac gccgctcgac gacggtgccg
     1081 ctcgtcctcc agtcgttcaa ctggacgcac ggcgtctacc tcggcgcgac catgggctcg
     1141 gagacgacgg cggccgccgt cgggaaggaa ggcgtcgtcc gccgcgaccc gatggcgatg
     1201 ctccccttct gcggctacga catggggcgc tacttcgagc actggctgaa catggagggg
     1261 aaggtcgcca cgctcccgaa gatcttcatg gtgaactggt tccggaaggg gcccgacggc
     1321 tcgttcctct ggcccggctt cggcgagaac atgcgcatcc tgaagtggat catcgaccgc
     1381 gcccgcggcc gcgccggcgc ctccgagagc gcgatcggct gggtgccgaa gtgggccgac
     1441 ctcccgctca aggacctcgc cgtcccgatg gaggacgtcg aggacgccca gacgatcgac
     1501 tacgaggagt ggaagaagga gctcgccgac caggacacct tcttcgagca gttctcctcg
     1561 acgatgccga aggagatcgt ctacgagcgc gagatcatcc gctcgcggct caaccgcgtg
     1621 acggcgaagt aggggccggg agaccagaga aacccggccg aggcaccaga gcaccagagc
     1681 accggagcac cggagcacga gggcacgaag gcaccggagg agggagcttc accccggggg
     1741 tgggtcccac ggccgctcgc cgggggggct gagcttcgcg cgccccccgg acccccgctc
     1801 tctcctcaac ttacacttcc attcttcaag cttcctggct gcgcgcggcc gacccgcggg
     1861 gcgtgcggcg ctccgcgctc gggtcgctcg cgaactcgct cgccttcggc gcgctcaaac
     1921 agcgctcgct ttcccccgag cgcctcgcgg cgcgcgccag cgggtccccg gccgtgctcg
     1981 agtcggctcg caggacccac ccccggggct cagcccaccg gagcgagaac cgagtggatg
     2041 ccgcttaaac agggatcttc ggcaggcgtt tccggccagg gcgaacgccg cgcaacacgt
     2101 tgacgtgcga gagccgccga tgagccggaa tctcccgtcc tcggctgtag gacgctcgac
     2161 ctcccccggc cgcggaaggg gcggggtgtc tcgcgagcca cccccgagca cggccgggga
     2221 cccgctcgcg ctcgcgagga ccgggcgggg atcaagcccg cgcgcatgtc tgagcgaagc
     2281 gagttcgcgc gggcgccccg cccggccgca gcgagtgcgg aagcgggtcg gccgcgcgcc
     2341 gacagcatct gaactccaga cgagcgcagc ttggaggagg gggcccgggg ggccgcgcga
     2401 cgctcaggcc ccccggcgag cggtggccgc gagacagccc gcaccggacg cggccccctg
     2461 ccgtggggac gcaacggcga agctggtgtc tcccggtccc ccggtctacc ggtctccggg
     2521 tgccccggcc cctcgcccta ctccgctctc cccccggtca gccgcgcttc ttccactcct
     2581 ccgtcgcgtc gacgagggcg gagacgatgc cgggctcgtc ggcggagtgg ccggaggcgg
     2641 ggacgatcac gaggcgcgag ccgggccagg cctggtggag gtcccacgcg gagacggccg
     2701 gacagaccat gtcgtagcgg ccctggacga tgacgcaggg gaggtgcttc accttctcga
     2761 cgtcacggag gagctgctcg tcgtccgaga ggaaagcgcc gttcacgaag tagtgcgcct
     2821 cgatccgggc gagagcgacc tcggcgggaa cgtccgcctc ggccaccgag tagttgtgag
     2881 cgacgagata cgagccgagc tcctcccagc ggttccaggc gagggccgcc tggcggcgga
     2941 cgacggggtc ctccgcgttc agtcggcgat ggtaggcggc caggacgtcg tccttctccg
     3001 attcgtccag gttcgagagg aactccgccc agccgtcggg gaagacccgc tgcgcgccgc
     3061 cgccgaacgt ccagaggacc tcggagcgac gggagaggaa gatcccgcgg aggatcacgc
     3121 ccgtgcaacg ttccgggtgc gtctcggcgt aggcgagtcc gagcgtggag ccccaggagc
     3181 cgccgaagac gagccacttc tcgatgccga gccgctcgcg gatccgctcc gcgtcggcga
     3241 cgaggtccca ggtcgtgttc tcccgcagct cgccgagagg cgtggaacga ccggagccgc
     3301 gctggtcgaa gaggatcacc cgataggccg ccgggtcgaa gaaccggcgg tgaagaggtg
     3361 agatccccgc ccccgggccg ccgtggaaga agacgacggg caggccgtcc gggctcccgc
     3421 tttgctcgac gtggagctcg tgcaggtcgg agacgcggag gcggaaggag tcgaacggct
     3481 cgatgggggg aaacaggtcc ggcacggcga gactatacgg cggaccggag aattggttcg
     3541 gcggcacgtt tgcagccccc cgacggcccc cgcctttcgt aaactccgtc gccatgccga
     3601 cggccactgc caggaaatcg tccgcccgct ccgccaagaa gggcccccgg acgcccgacc
     3661 gcttcgcgga ggcggaggag ctctacggga tcgacgcgtg ggggaagggg ttcttctcca
     3721 tctccgacga cgggcacctc ctcgtccacc cgacgcggga gggccaccgc ttcgccgacc
     3781 tgaaggacgt cgtcgacgag gtcgggggac gcgggatcac ccctccgatg atcatccgct
     3841 tcccgcagat cctcgcgtcg tcggtgcggg agctgaacga ggccttcggg aaggcgatca
     3901 aggagttcgg ctacgacggc gactaccggg gcgtcttccc gatcaaggtg aaccagaaga
     3961 aggtcgtcgt ccacgagatc atcgaggccg gccggaagta cggctacggc ctcgaggccg
     4021 ggtcgaagcc cgagctgatc gccgccctct cgcaggacct cggccccgag tgcctcatca
     4081 cgacgaacgg ctacaaggac gaggcgttca tccggctcgc cctcgacggc gtgaagatgg
     4141 ggcggaacgt catcctgacc ctcgagaagg tctccgagct cgagagggtc ctcgaggtcg
     4201 cgaagaagcg cggggtcaag ccgctcgtcg gcatgcgcgc gaagctctac gcccgcggct
     4261 ccggcaagtg ggccaagtcg gggggcgagg cggcgaagtt cggcctgacg acgacggaga
     4321 tgctcgcggc gatcgagatc ctgaagtcga agcggatgct cgactcgctc gtcatgctcc
     4381 acttccacat cggctcgcag atcaccgaca tccggaagat caaggacgcg atccgcgagg
     4441 cggggcgcgt ctacgcgaag ctgagccgct ccggcgtcga agtgcgctac ctgaacctcg
     4501 gcggcggcct cggcgtcgac tacgacggct cgaagacctc gttcgactcc tcgatgaact
     4561 acaccgtcca ggagtacgcg aacgacgtcg tctacaacat caagacgatc tgcgaggacg
     4621 agaaggtccc ggtgccgacg ctcgtgaccg agtcggggcg ggccgtgacg gcctaccact
     4681 ccgtcctcgt ctcgaacgtc ctcgacgtgg ccgacaagat cgagcaggac aagaccgtca
     4741 cgctgaacgg cgacgagaac cacgtcgtcc gcgagctctg ggacgtcttc aagggcgtga
     4801 cggcgaagaa cgtccgcgag agctaccacg acgccctcca gtacaaggag gagctcttca
     4861 ccctgttcaa cctcggctac gtctcgctcg aggaccgctc gaagggcgag atcctcttct
     4921 gggacgtctg cgacaagatc cggaggcacc tgaaggggct ccgggagatc cccgaggagt
     4981 tcgaggacct cgagcgcgag ctcgcggaca agtacgtcgt caacttctcc gtcttccagt
     5041 cggtccccga ctcgtgggcg atcgaccagc tcttcccgat cctcccgatc caccggctcc
     5101 gcgagaagcc gggcgagatc gcgacgctcg ccgacatcac ctgcgactcg gacgggaaga
     5161 tcgagaagtt catcgacctg cgcgacatca aggagttcct cccgctccac tcctggaagc
     5221 gctcgcagcc
//
LOCUS       DAOTEZ010000316         5172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_11607_length_5172_cov_6.015830, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000316 DAOTEZ010000000
VERSION     DAOTEZ010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5172
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11607_length_5172_cov_6.015830"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="P5164_14725"
     CDS             complement(<1..250)
                     /locus_tag="P5164_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY45196.1"
                     /translation="MSVAPETDLIVFEEVSKFYGEVLGVDRVTLTIPPGITGLVGPNG
                     SGKTTLMNLATGLIRPTRGRVRVLGIPPDDPESLFSACG"
     gene            complement(247..1107)
                     /locus_tag="P5164_14730"
     CDS             complement(247..1107)
                     /locus_tag="P5164_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45197.1"
                     /translation="MTVRARRFRPFEGTPTSTLTRLLVLPRYAWKEAFAGKLVTGAFT
                     SAFLVPAGALVLVWLKHNIAALVNTPFAGGIPLPIDAQFFGWLLVAQSYFAFALTLFV
                     GPSLVSPDLVNGALPLYFSRPISRWQYVAGKLLVLAALLSAITWAPLLLVFVLQASLA
                     DGSWIAGHAGIAGSILLGSLLLVLFLSLVSMAVSAWVRNRAVARGLLIGLGVVPAVFG
                     QALNATLDVEWGGVLNIGLAWGRVLEGLFGIPVRAGIPLGGAWASLLTATALALLLLR
                     RKLKAFEVVR"
     gene            complement(1104..2024)
                     /locus_tag="P5164_14735"
     CDS             complement(1104..2024)
                     /locus_tag="P5164_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY45198.1"
                     /translation="MTAFLELEGLSVRLGRRDVLRDLTGTLAGRAIGLLGPNGAGKTT
                     LLHTLLGFFRPSSGTARLAGLDVVRESREVRRAIGYMPENDAFVAGISGVRYVAMFAE
                     LSGLPRAEALERAHEALFSVGLGEVRYRNLETYSLGMKQLAKLAQAIAHGPRVLFLDE
                     PTNGLDPPARQRMIRLIKEIREAGEIRVVLSSHLLRDVEECCDEVLILRDGRIAALCD
                     LEAERRANRRLVELEAVGGNGDFAGALAGLGCAVAPGIRRRLKVVLPDGVGPRELYRL
                     AGETGVQLRHLTYQRDSLEEIFQRAMENGG"
     gene            complement(2021..4576)
                     /locus_tag="P5164_14740"
     CDS             complement(2021..4576)
                     /locus_tag="P5164_14740"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRY45199.1"
                     /translation="MRDDIVEKAVEAVRTQEPDSAAVGAALARVAPAVHAAPVEPVAA
                     VEAPAVFRSCADLQALLPAFVARSLSPDRALLVEDHTRSCVPCRRALKNLREGTPSES
                     PAAPAARRRPYGAWALAASVVLAAALGTFWLLSNRAAAGPVARVDVVDGLLLLPAEGR
                     PLAPGAELSGGQPVRTGRSGGAVLVLADGSRVELADRSQVSIARGAGGTTVSLARGRV
                     IVQAAKQRTGHLFVRTDDSLVSVTGTVFAVAKGTKGTRVSVLEGEVHVDDGAGEKVLH
                     GGDQTATQGIVARVSLEEEFSWSRDRVALLELAGEVARVQKQLAAVPAPWSGRTSTRL
                     LDAMPDGTVLYAALPNISGTVADFYDALAPRLAANPVLASWWSEGPGAAERQAEVKKL
                     LDTLRTWGSYLGDEVAVGIAKDASGHPGAPVALTTVVKAGFREFVEAEIARAGAGAKV
                     RIVGSSAEAAEDTLNLWLRDDLLVAAPNAGALARLESALAAPGTFRSGTFHARLAEAY
                     RDGVDLLVGVDAKSLLARAASGADAESGAFLRMSGLADVEHLIVERRSAAGASSGRAA
                     LTFGAERRGMAAWIAPPGPMGALSFVSSSASGAVAFVTKEPSLLVGDLFAMLTDGQPD
                     FPAKLAEAEAKLGFRLRDDLAAALGGDAALAVDGPLLPTPALKLVVEVYDPARLQATI
                     VKLVSLADAEARKAGKPGVSLATETVSGLTVHSLVAGAAPSRLQYAFVNGYLVVAPEK
                     ALLVRAAEAHATGDTLLTAPKLVSLLPKDGPLDFSMLAFQDVGGALGALANAVGAAPG
                     SAASELSGSGASLAWAWAEPTRVVFGSSGAGLTELGSLVAAGAALNAPASGGR"
     gene            complement(4573..5121)
                     /locus_tag="P5164_14745"
     CDS             complement(4573..5121)
                     /locus_tag="P5164_14745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRY45200.1"
                     /translation="MSAARPIPRPVPPTPLEEVFREYGDLVFRTAFRVSGSAADAEDV
                     LQTVFLRLASPGKAERLGAEPRGYLQRAAVNGALDLVRTRRSAAGTVPIEERADAFLA
                     KEPLPDAVSEARELRGRLRRALGKLAPRPAEVFVLRAVEGLTNREVARLLGSSPATVA
                     VQYFRARTQLRKELLAEGGGPS"
BASE COUNT          763 a         1786 c         2047 g          576 t
ORIGIN      
        1 agccgcaggc ggagaagagg ctctcggggt cgtcgggcgg aatgccgagg acgcggacgc
       61 gcccgcgcgt gggacggatc aggcccgtcg cgaggttcat gagcgtcgtc ttaccggagc
      121 cgttcgggcc gacgaggccg gtgatcccgg gagggatcgt gagcgtcacc cggtcgacgc
      181 cgaggacctc gccgtagaac ttcgagactt cctcgaagac gatgaggtcc gtctcgggcg
      241 cgacgctcac cggaccacct cgaacgcctt cagctttctc cgaaggagga ggagggcgag
      301 cgccgtcgcc gtcaggaggc tcgcccaggc ccctccgagc gggattccgg cccggaccgg
      361 aatgccgaag agcccctcca ggacgcgccc ccaggcgagg ccgatgttca gcaccccgcc
      421 ccactcgacg tccagcgtcg cgttcagcgc ctggccgaag acggccggca cgacgccgag
      481 gccgatgagg aggccgcgcg cgaccgcccg gttcctcacc caggcggaaa ccgccatcga
      541 gacgagcgag aggaagagga cgaggaggag cgacccgagg aggatcgacc cggcgatgcc
      601 ggcgtgcccg gcgatccagc tcccgtcggc gagcgaggcc tggaggacga agacgaggag
      661 gagcggggcc caggtgatcg ccgagaggag cgccgcgagg acgaggagct tgccggccac
      721 gtactgccag cgcgagatgg ggcgcgagaa gtagagcggc agcgcgccgt tcacgaggtc
      781 gggcgagacg agggaggggc cgacgaagag cgtgagggcg aaggcgaagt acgactgcgc
      841 cacgaggagc cagccgaaga actgcgcgtc gatggggagc gggatccccc cggcgaacgg
      901 cgtgttcacg agcgcggcga tgttgtgctt cagccagacg aggacgaggg ccccggcggg
      961 gacgaggaac gccgaggtga acgccccggt gacgagcttt cccgcgaacg cctccttcca
     1021 ggcgtagcgc ggcaggacga ggagccgcgt gagcgtcgac gtcggcgtcc cctcgaaggg
     1081 gcggaagcgg cgcgcgcgga ccgtcatccg ccgttctcca tcgccctctg gaagatctcc
     1141 tcgagcgagt cgcgctggta cgtcaggtgc cggagctgga ctcccgtctc gccggcgagc
     1201 cggtagagct cgcgggggcc gacgccgtcg gggaggacga ccttcagccg ccggcggatg
     1261 ccgggcgcca cggcgcagcc gaggcccgcg agcgcgccgg cgaagtcgcc gttcccgccc
     1321 acggcctcga gctccacgag gcgccggttc gcccggcgct ccgcctcgag gtcgcagagc
     1381 gccgcgatcc ggccgtcgcg gaggatgagg acctcgtcgc agcactcctc gacgtcgcgg
     1441 aggaggtggg acgagaggac gacgcggatc tcccccgcct cgcggatctc cttgatgagc
     1501 cggatcatcc gctgccgcgc cggagggtcg aggccgttcg tcggctcgtc gaggaagagg
     1561 acgcgcgggc cgtgggcgat cgcctgcgcc agcttcgcga gctgcttcat cccgagcgag
     1621 taggtctcga ggttccgata ccggacctcg ccgaggccga cggagaagag cgcttcgtgc
     1681 gcccgctcga gcgcctcggc gcgcgggagg cccgagagct ccgcgaacat cgcgacgtag
     1741 cggacgcccg agattcccgc gacgaaagcg tcgttctcgg gcatgtagcc gatcgcccgc
     1801 cggacctccc ggctctcccg cacgacgtcg aggcccgcga ggcgggccgt tcccgacgac
     1861 gggcggaaga agccgagaag ggtgtggagg agcgtcgtct ttcccgcgcc gttcggcccc
     1921 agaaggccga tggcccggcc ggcgagcgtg cccgtcaggt cccggagcac gtcccgccgg
     1981 ccgagccgga cggaaaggcc ttcgagctcg aggaaggcgg tcaccggcct ccgctcgcgg
     2041 gggcgttcag ggcggccccg gccgcgacga gggagccgag ctcggtcagg ccggcgccgg
     2101 aggagccgaa gacgacgcgc gtcggctcgg cccaggccca ggcgagcgag gcgccggagc
     2161 cggagagctc cgaggcggcc gagccgggag cggcgccgac ggcgttcgcg agggccccga
     2221 gcgcgccgcc gacgtcctgg aaagcgagca tcgagaagtc gagcggcccg tccttcggga
     2281 ggagcgagac gagcttcggc gccgtcagga gcgtgtcgcc cgtcgcgtgc gcctcagcgg
     2341 cgcggacgag gagcgccttc tccggggcga cgacgaggta gccgttcacg aacgcgtact
     2401 gtagacgaga gggagcggcg ccggcgacga gcgagtggac ggtcaggccc gagaccgtct
     2461 cggtcgcgag cgagacgccc ggcttcccgg ccttccgcgc ctcggcgtcg gcgagggaga
     2521 cgagcttcac gatcgtggcc tggagccggg cggggtcgta gacctcgacg acgagcttca
     2581 gcgcgggcgt cgggaggagc gggccgtcga ccgcgagcgc ggcgtccccg ccgagcgcgg
     2641 cggcgaggtc gtcccggagg cggaagccga gcttcgcctc ggcctcggcg agcttcgccg
     2701 ggaagtcggg ctggccgtcc gtcagcatcg cgaaaaggtc gccgacgagg agggacggct
     2761 ccttcgtcac gaaggcgacc gcaccggagg cgctcgagga gacgaaggag agggcgccca
     2821 tcgggccggg gggtgcgatc cacgccgcca ttccgcggcg ctcggcgccg aaggtgagcg
     2881 ccgcgcggcc gctcgaggcg cccgccgcgg agcggcgctc cacgatcagg tgctcgacgt
     2941 cggcgagccc gctcatccgg aggaatgcgc cgctctccgc gtccgccccg gacgcggccc
     3001 gggcgaggag cgacttcgcg tcgacgccga cgaggaggtc gacgccgtca cggtaggcct
     3061 cggcgaggcg ggcgtggaac gtcccgctcc ggaacgtgcc gggtgcggcg agcgccgact
     3121 cgaggcgcgc gagcgcgccg gcgttcgggg ccgccacgag gaggtcgtcc cggagccaga
     3181 ggttcagggt gtcctcggcc gcttcggccg agctcccgac gatccggacc ttcgcgcccg
     3241 cgccggcgcg ggcgatctcg gcctcgacga attcgcggaa gccggccttt acgaccgtcg
     3301 tcagcgcgac cggcgcgccg ggatggccgg aggcgtcctt cgcgatcccg acggcgacct
     3361 cgtcaccgag gtaggagccc caggtgcgga gcgtgtcgag gagcttcttc acctcggcct
     3421 gccgctccgc ggcgcccggc ccctcgctcc accaggaagc gaggacgggg ttcgcggcga
     3481 ggcgcggggc gagggcgtcg tagaagtcgg ccacggtgcc ggagatgttc gggagcgcgg
     3541 cgtagaggac ggtgccgtcc ggcatcgcgt cgaggagacg cgtcgaggtc cggcccgacc
     3601 agggggccgg gaccgccgcg agctgcttct gcacgcgggc cacctcgccc gcgagctcga
     3661 ggagggcgac ccggtcgcgg ctccaggaga actcctcctc gagggagacc cgggcgacga
     3721 ttccctgcgt cgccgtctgg tcgccgccgt ggaggacctt ctcgcccgcg ccgtcatcga
     3781 cgtggacctc cccctcgagg acggagacgc gcgtcccctt cgtccccttc gcgacggcga
     3841 agaccgtccc ggtgacggag acgagcgagt cgtcggtcct cacgaagagg tgtccggtcc
     3901 gctgcttcgc cgcctggacg atgacgcgtc cgcgcgcgag ggagacggtc gtgccccccg
     3961 cgccgcgcgc gatggagacc tgggaacgat cggcgagctc gacgcgggag ccgtcggcca
     4021 ggacgaggac ggcgccgccg gagcgccccg tccggaccgg ctggccgccg gagagctctg
     4081 cgcccggcgc gaggggacgc ccctcggccg gcaggaggag gaggccgtcg acgacgtcga
     4141 cgcgggcgac cggcccggcg gcagcgcggt tggagaggag ccagaaggtc ccgagcgccg
     4201 ccgcgaggac cacggaagcc gcgagcgccc aggcgccgta gggccggcga cgcgcggcgg
     4261 gggcagcggg cgactcggac ggggttccct cgcggaggtt cttcagcgcc ctccggcacg
     4321 ggacgcagga gcgcgtgtgg tcctcgacga gaagcgccct gtccggagag aggctccggg
     4381 cgacgaacgc ggggaggagc gcctgaaggt cggcgcagct ccggaagacg gccggcgcct
     4441 cgacggcggc gaccggctcg acgggagccg cgtgaacggc gggcgcgacg cgggcgagcg
     4501 cggcgccgac ggcggcggaa tcgggctcct gggtgcggac ggcctcgacg gccttctcga
     4561 cgatgtcgtc tctcatgacg ggcctccccc ttcggcgagg agctccttgc ggagctgggt
     4621 tcgggcgcgg aagtactgga cggcgacggt cgcgggtgac gacccgagca ggcgggccac
     4681 ctcgcggttc gtgagcccct cgaccgcgcg gagaacgaag acctccgcgg ggcggggggc
     4741 gagcttcccg agcgcgcgcc ggagacggcc ccgaagctcg cgggcctcgg agacggcgtc
     4801 ggggagcggc tccttcgcga ggaacgcgtc ggcgcgctcc tcgatcggga ccgtccccgc
     4861 cgccgaccgc cgggtcctga cgaggtcgag ggccccgttc acggccgcgc gctggaggta
     4921 gccgcggggc tcggcgccga gccgctcggc cttccccggc gaggcgaggc ggaggaagac
     4981 ggtctggagg acgtcctcgg cgtcggcggc gctcccggag acgcggaacg ccgtccgaaa
     5041 gacgaggtcg ccgtactccc ggaagacctc ctcgaggggt gtcggaggaa cgggccgggg
     5101 aatcggtctc gccgcgctca cgggtgcctc ctgtctcggg tcatcaccca tgaaacggac
     5161 ctcctcccga at
//
LOCUS       DAOTEZ010000317         5167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_11625_length_5167_cov_7.010563, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000317 DAOTEZ010000000
VERSION     DAOTEZ010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11625_length_5167_cov_7.010563"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="P5164_14750"
     CDS             complement(<1..578)
                     /locus_tag="P5164_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY45201.1"
                     /translation="MSDPAVRVRDLVKTFEVTKKAPGLSGALKGLFRAERGTLTAVDR
                     VSFELVRGEMVGYIGPNGAGKSTTIKMLTGILTPTSGEVSCLGFVPWRERRAYARSIG
                     VVFGQRTQLWWDIAVVESFQLLREIYGVSGADYERRMKVFDDLLEIGRLLSQPVRKLS
                     LGERMRCDLAASLLHEPPLLFLDEPTIGLDVVA"
     gene            complement(575..1369)
                     /gene="rsmI"
                     /locus_tag="P5164_14755"
     CDS             complement(575..1369)
                     /gene="rsmI"
                     /locus_tag="P5164_14755"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRY45202.1"
                     /translation="MASPPPAAGRLLVVATPIGNLEDLSPRALAALREAALVYCEDTR
                     RTGNLFARHGLSTARVSCHEHNEFRRIPEMLERLARGETLALVSDAGTPGVSDPGRLL
                     AAAAAEAGHRVEPVAGPSAVAAILSVCGFPAVPFSFLGFPPSRRGERARWYARFAARD
                     ETRVLFESPFRVVESLEEMARAWGDPRVCLGRELTKLHEEVLRGTASSVAALLGRRPG
                     VKGEIAIAAAPSAAAVAAHPGVGADLADDAEREGGPEGDDAAGTDE"
     gene            1454..2152
                     /locus_tag="P5164_14760"
     CDS             1454..2152
                     /locus_tag="P5164_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45203.1"
                     /translation="MRWLALLALLALGAAGEAPSRTTGPAVTLDRIAAVVGDEIVLEG
                     EVSRLAAIGFLPRREGEAELAYRDRLLDLRVVELLREKELRLLTGLEPDPAEVDARLD
                     EVAARYEAGAGEPFERALERARTGRDEVRGWIRRGMALESYARERLLPTVRVTEPAMR
                     AFYDGPFRAEAESRGLTTLPPFSEVQDQLRELLRERLLNEEVAKWTEGLREKTRILVY
                     RRPPIASASGSASR"
     gene            complement(2104..4521)
                     /locus_tag="P5164_14765"
     CDS             complement(2104..4521)
                     /locus_tag="P5164_14765"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRY45204.1"
                     /translation="MTGVRGGARLAALAASLLISGAAPAAGPLPEPTVAAAPAKLSGE
                     ALRLLEREAAEAARAEDWVDVLAHLHEIYRRAPDRYMDGRFEFLTARALAGLGRVDEA
                     LVRFERFAAPGDLFDVPARLAAARLRFGRGDGTEALDLLLPLLQRKEGAISRRALRTA
                     LDALETRFDAAALARLVAARPQTPPRERRRLQALRAEALEREGAATEAAALRISLLAE
                     GRRDDAAAALLARELAGRTPADHVDATLRLLVDTARSQRDLELAERLAAERERRAVAG
                     PDAAEAATARFELARLRGSRGRFAEAASGFAALLDATPKGLRPPARKGDDGFGTASFA
                     GRVRFNLGAMKEKLGDLDGAVEEFRRVEAGGAGPVGLSVLQRARLEIRRGNLDRAETL
                     LLRPSLAGEPGRVEALLLLVERRAQSRDGAGATRALAPVAVLAKAHRLPEPWLSELPF
                     WTGLVARARNDVATALSEWAGLLAVDPGSFAAERALARIEELPAASRERFVASARADG
                     RRLLRAGRAAAAKDRLLPAAALGDAEAKEALREAYRALPRYADVLLAPELPDESLPDL
                     CGDAAACRLLQLGLAEDAEPIVREARRLDTLLGSLVAARLAEDAGAGPAALEAAEAVS
                     RKVPRDFLLELAPRTIRRGLAPRPFDRLVAQAAAELGVPRDLLYAIMRQESRFDREAA
                     SPAAARGLMQLTLPAAGEAARELNEAPPAYSDLYDPARSIRLGARTLRSLLDRFDGDA
                     TRAASGYNAGAGQTVLWSGGSDRPAEALLAAISYAETRTYVRRVLANRRAYRLALPEA
                     EAIGGRR"
     gene            complement(4518..>5167)
                     /locus_tag="P5164_14770"
     CDS             complement(4518..>5167)
                     /locus_tag="P5164_14770"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465977.1"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRY45205.1"
                     /translation="VSGAGTETVVVEGVDALSGTPAPHAVLADRIEAGTYAAAAAITG
                     GDVTLGGARETDLPAFVSALRAAGAEVESTDAGLRVARSGPLRAVDVETAPHPGYPTD
                     LQAQWMALMTQAEGTSRIVETIFENRFLHAAELVRLGANVRVDGRTAHVAGPSRLEGA
                     TVTASDLRASAALVLAALVAEGETLVRRIYHLDRGYAAMEEKLSSLGARVARVSG"
BASE COUNT          650 a         1812 c         2130 g          575 t
ORIGIN      
        1 gcgacgacgt cgaggccgat cgtcggctcg tcgaggaaga ggagcggggg ctcgtggagg
       61 agcgaggcgg cgaggtcgca gcgcatccgc tcgccgaggg agagcttccg cacgggctgc
      121 gagagcagcc ggccgatctc gaggaggtcg tcgaagacct tcatccggcg ctcgtagtcg
      181 gcgccggaga cgccgtagat ctcgcggagg agctggaagc tctcgacgac ggcgatgtcc
      241 caccagagct gggtccgctg gccgaagacg acgccgatcg acctcgcata ggcccggcgc
      301 tcgcgccagg gcacgaagcc gaggcagctc acctcgcccg aggtcggcgt caggatgccc
      361 gtgagcatct tgatcgtcgt cgacttgccc gcgccgttcg ggccgatgta gccgaccatc
      421 tcgccgcgca cgagctcgaa ggagacgcgg tcgacggcgg tgagcgttcc gcgctcggcg
      481 cggaagagcc ccttgagcgc cccagagagg ccgggggcct tcttcgtcac ctcgaacgtc
      541 ttgacgaggt cgcggacgcg gacggcgggg tcgctcattc gtccgtgccg gcagcgtcgt
      601 cgccctcggg cccgccctcc cgctccgcgt cgtccgcgag gtcggcgccg acgcccgggt
      661 gcgccgccac ggccgcggcg gagggcgccg cggcgatcgc gatctccccc ttcacgccgg
      721 gccgcctccc gaggagcgcg gcgacggacg aggccgtgcc gcgcaggact tcctcgtgga
      781 gcttcgtcag ctcccggccg aggcagaccc gcggatcacc ccaggcgcgc gccatctcct
      841 cgagcgactc gacgacgcgg aaaggagact cgaagaggac ccgcgtctcg tcgcgcgcgg
      901 cgaagcgggc gtaccagcgg gcgcgctcgc cgcggcgcga gggagggaag ccgaggaagg
      961 agaaggggac ggccgggaag ccgcagaccg agaggatcgc ggcgacggcg gagggaccgg
     1021 ccacgggctc gacgcggtgg ccggcctcgg cggcggcggc ggcgagaagc ctcccggggt
     1081 cggagacgcc cggggttccc gcgtcggaga cgagggccag cgtctcgccg cgcgcgagcc
     1141 gctcgagcat ctccgggatc cggcggaact cgttgtgctc gtggcaggag acgcgcgcgg
     1201 tcgacaggcc gtggcgcgcg aagaggttcc cggtgcgacg cgtgtcctcg cagtagacga
     1261 gggccgcctc gcggagcgcc gcgagggcgc gcggcgagag gtcttccagg ttgccgatcg
     1321 gagtcgcgac gacgaggagc cgcccggcgg cggggggagg cgaggccacg ccgggatgct
     1381 accagcgagg tccgccgggg cgaggcgcgc ccccgccgga gacgctcccg cgggcggcgg
     1441 tacgatcggg gggatgaggt ggctcgctct cctcgcgctc ctcgcgctgg gcgcggccgg
     1501 agaggctccg tcccggacga cggggccggc ggtgacgctc gaccggatcg cggcggtcgt
     1561 cggcgacgag atcgtcctcg agggcgaggt ctcgcgcctc gcggcgatcg ggttcctgcc
     1621 tcggcgcgag ggagaggccg agctcgccta ccgcgaccgg ctcctcgacc tgcgggtcgt
     1681 cgagctgctg cgggagaagg agctgcgcct cctgacgggc ctcgagcccg acccggccga
     1741 ggtcgacgcg cggctcgacg aggtcgccgc gcggtacgag gcgggggcgg gcgagccgtt
     1801 cgagcgggcg ctcgagcggg cccgcacggg ccgcgacgag gtgaggggct ggatccgccg
     1861 ggggatggcg ctcgagagct acgcgcgcga gaggctcctc ccgacggtgc gggtgaccga
     1921 gccggcgatg cgcgcgttct acgacggtcc gttccgggcc gaggccgaga gccggggcct
     1981 gacgacgctt cctccgttct ccgaggtgca ggaccagctg cgcgagctcc tccgcgagcg
     2041 gctcctgaac gaggaggtcg cgaagtggac ggaggggctc cgcgagaaga cgcggatcct
     2101 cgtctaccgg cgtccgccga tcgcctcggc ctcgggcagc gcgagccggt aggcgcggcg
     2161 gttcgcgagg acgcgcctca cgtaggtccg ggtctcggcg tagctgatgg cggccaggag
     2221 cgcctcggcc ggccgatcgc tcccgccgct ccagaggacg gtctgccccg cgccggcgtt
     2281 gtagcccgag gcggcgcgcg tggcgtcgcc gtcgaagcgg tcgaggagcg agcggagcgt
     2341 ccgcgcgccg aggcggatcg agcgagcggg gtcgtagagg tcggagtagg cggggggcgc
     2401 ctcgttcagc tcgcgcgcgg cctcgcccgc ggcggggagc gtgagctgca tcaggccgcg
     2461 tgcggccgct ggcgaggccg cctcgcggtc gaaccggctc tcctggcgca tgatggcgta
     2521 gaggaggtcg cgcgggacgc cgagctcggc ggccgcctgc gcgacgagcc ggtcgaaggg
     2581 gcggggggcg agcccgcggc ggatcgtccg cggagcgagc tcgaggagga agtcgcgcgg
     2641 gaccttcctc gagaccgcct cggccgcttc gagggcggcc gggccggcgc cggcgtcctc
     2701 ggcgagccgg gccgcgacga ggctcccgag gagcgtgtcg aggcggcgag cctcgcggac
     2761 gatcggctcg gcgtcctcgg cgaggccgag ctggaggagg cggcaggcag cggcgtcgcc
     2821 gcagaggtcc gggagggact cgtcgggaag ctccggcgcg aggaggacgt cggcgtagcg
     2881 cgggagcgcg cggtaggcct cgcggagagc ctccttcgcc tccgcgtcac cgagggcggc
     2941 ggccgggagg aggcggtcct tcgccgccgc ggctcttccc gcccggagga ggcgccgacc
     3001 gtcggctcgg gccgatgcga cgaacctctc gcggctcgcg gcggggagct cctcgatccg
     3061 ggcgagagcg cgctccgcgg cgaaggaacc gggatcgacg gcgaggagcc cggcccactc
     3121 ggacagtgcg gtcgccacgt cgttgcgggc ccgggccacg aggccggtcc agaacggcag
     3181 ctcggagagc cacggctccg gcaggcggtg cgccttcgcg aggaccgcga cgggggcgag
     3241 cgcgcgcgtg gcgccggcgc cgtcgcgcga ctgggcgcgc cgctcgacga ggaggaggag
     3301 cgcctcgacg cggcccggct cgccggcgag cgaggggcgg agcagcagcg tctcggcccg
     3361 gtcgaggttc ccgcgccgga tctcgaggcg agcccgctgg aggacggaga ggccgacggg
     3421 gccggctcct ccggcctcga cccggcggaa ctcctcgacg gcgccgtcga ggtcgccgag
     3481 cttctccttc atcgcgccga ggttgaagcg aacgcgcccg gcgaacgagg cggtgccgaa
     3541 gccgtcgtct cccttccggg ccggtggccg gagacctttc ggcgtcgcgt cgagcagagc
     3601 tgcgaagccg ctcgccgcct cggcgaaccg gccgcgcgag ccgcggaggc gggcgagctc
     3661 gaagcgggcc gtggccgctt ccgcggcgtc ggggcccgca acggcccgcc tctcccgctc
     3721 ggcggcgagc ctctcggcca gctcgaggtc cctctgcgac cgggcggtgt cgacgaggag
     3781 gcggagagtc gcgtcgacgt ggtcggcggg ggtccgtccc gcgagctcgc gcgcgagaag
     3841 ggccgcggcc gcgtcgtctc ggcgcccctc ggcgaggagg gagatgcgca gcgccgccgc
     3901 ttccgtggcc gctccctcac gctcgagcgc ctcggcgcgg agcgcctgaa gccggcgccg
     3961 ctcgcgagga ggggtctggg gacgggccgc gacgagccgg gcgagagcgg ccgcgtcgaa
     4021 gcgcgtctcg agggcgtcga gcgcggtccg gagggcccgg cgggagatgg ccccttcctt
     4081 cctctggagg agcgggagga ggaggtccag cgcctcggtc ccgtctcccc tgccgaagcg
     4141 aaggcgtgcg gcggcgaggc gggccgggac gtcgaagagg tcacccggcg ccgcgaagcg
     4201 ctcgaatcgg acgagcgcct cgtcgaccct tccgaggccg gcgagggcgc gcgccgtcag
     4261 gaactcgaag cggccgtcca tgtagcggtc cggcgcccgc cggtagatct cgtggaggtg
     4321 ggccaggacg tcgacccagt cctcggcgcg ggccgcctcg gccgcctccc tctcgaggag
     4381 gcgcagcgcc tcgcccgaga gcttcgccgg cgcggcggcc accgtgggct cggggagcgg
     4441 cccggcagcg ggcgcggccc cggagatgag gagcgaggcg gcgagcgccg cgagccgggc
     4501 gccgccccgc accccggtca ccccgagacg cgggcgaccc gggcgccgag cgaggagagc
     4561 ttctcctcca tcgcggcgta gccccggtcg aggtgataga tgcgccgcac gagcgtctcc
     4621 ccttcggcga cgagcgccgc gaggacgagc gcggcggagg cgcgcaggtc ggaggcggtg
     4681 acggtggcgc cctcgagccg cgaggggccg gcgacgtggg ccgtgcgccc gtcgacgcgg
     4741 acgttcgcgc cgaggcggac gagctcggcg gcgtggagga accggttctc gaagatcgtc
     4801 tcgacgatcc gcgaggtccc ctcggcctgc gtcatgagcg ccatccactg cgcctgcagg
     4861 tcggtcggat agccgggatg gggcgcggtc tcgacgtcga cggcgcggag gggtccgctc
     4921 ctcgcgacac ggaggccggc gtccgtcgat tcgacctcgg cgccggccgc ccgcagcgcc
     4981 gacacgaagg cggggaggtc cgtctcgcgc gcgccgccga gcgtcacgtc gcctccggtg
     5041 atcgcggcgg cggcggcgta ggtccccgcc tcgatccggt cggcgaggac ggcgtgggga
     5101 gcgggcgtcc ccgagagagc gtcgaccccc tcgacgacga ccgtctccgt ccccgccccc
     5161 gagacgc
//
LOCUS       DAOTEZ010000318         5153 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_11673_length_5153_cov_9.533346, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000318 DAOTEZ010000000
VERSION     DAOTEZ010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11673_length_5153_cov_9.533346"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1459
                     /locus_tag="P5164_14775"
     CDS             <1..1459
                     /locus_tag="P5164_14775"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRY45206.1"
                     /translation="EMRAEESKMRAAWRRGEVDDLMKPENKTARYKELEAKFDALVKE
                     QRDLLVKNEFDRIYTKNGASGMNAFTSYDMTAYFITVPKNKLELWFWMESDRLKDPVF
                     REFYAERDVVYEERRLRVESTPTGKLDEAFDSIFWHSTPYTWPVIGYASDVANITKQQ
                     ADDYFATYYAPNNLTAVLVGDFDKAEALKLATEYFGRIPRGKTPPPEVTTLPTKWEYD
                     LRFEGEADTNPTAEIRWRTVPFQHKDSYALSILAELLNGRTGRLYKSMVLPADAVATQ
                     AYAVHNGFVGPAKYAGSFVIGAEAKEGQLPPEVAAKALAEIERLKKEPVPADELRKVK
                     NNVAANSFRRLSSNFPILLQLMVNEAYGDWTEINSGPKKLEAVTAEDVARVAKTYLVK
                     ENQATALVSRKAGSAPSADDAALAEVPEQVRPMIKQALQQLAAEKDAAKLKEGLAQME
                     TQAAQVPPQMKKGFELLVTKARERLAELEKAGDKK"
     gene            1480..2991
                     /locus_tag="P5164_14780"
     CDS             1480..2991
                     /locus_tag="P5164_14780"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRY45207.1"
                     /translation="MKNLARTSALALLGALLLAPAAQGVPPHPDKLTYPKFTYQPPSA
                     KEYRTVLKSGPVAFVAEDRELPLVSINVALRGGTYLSPAGKEGLADLAGYLLARGGTA
                     TKTAEELEERLAFLAASLNSGYGPDRGNVSLNLLAKDLDEGLAILREVLTAPRFQDDK
                     LKLRRDQLLNDMKSRNDDTADIEQRERGFLAFGEGYYTNRYTTKASLESITREDLLAF
                     HRRWVDPRNVTVAVAGDFGKGVSRAQMIAKLDALFAKWPYKGEAAPPVPTPTHAMAPG
                     LYVVDKDVNQGRVSVMLPALARTDPDYDAALVMNDVLGGGGFTSRITNRVRSDEGLAY
                     SAGSGIVGGVWYPATFRASFQSKARTCAYATQIVLEEMTKVRDAEVTDEELQTAKSSF
                     IDTLSRNFATKAQTVNVFLDEEFTGRFAKDPDYYRNYAARIEKVTKADVQRVARRLLA
                     TDKVAVLAVGKKDDLLNPDPKHPVKFPELTGGKLTDLPLRDPFTMKPLAPAAK"
     gene            3082..>5153
                     /locus_tag="P5164_14785"
     CDS             3082..>5153
                     /locus_tag="P5164_14785"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HRY45208.1"
                     /translation="MRATLPVLPAALLALFLGSPGAVLGGPPAGRAALTLERVHAAEP
                     LVEPAPTGLRWRDASRFTYLKRESSEPGARASLWQEDAATGKAGKLLDAIPAEGTGKD
                     GTPRSLPLSDASWNEAGTALVVVAENDLWLYAFGAGPARRLTSDADAEELPVFSPDGT
                     KVAFVKGNDLWFVEVGTGRATRLTTDGSATVLNGRLDWVYEEELAGRNGGRAYEWAPD
                     SSAIAFLRLDVSKVPEYPLVDFLPTNGKLLPQRYPKPGDPNASPSVRVVTWDSSGLAT
                     TSRSVSYDGERDLLGPELAWTPDSSAVAFTRMDRTQTAIEAFLLDRSGSAGPRSLLRE
                     SSASWVNAHEPPIFLPDGSGFLWLSERSGFLHLYRYGRDGTLLGPVTRGEWAITDTPR
                     LDARAGSVTFTATEADPRERHLYRVRLDGTGLTRLTSGKGTHAAIPSPDGRWVVDSWS
                     AVDTPTRVDLARGDGTPLRPVFVPKTSLGDFALATTEMGSFRGSDGTLFYTRLVKPAG
                     FDPARKYPVVVYVYGGPHAQVVQERWGATSLLDHVLAANGFLVWAMDGRGSGGRGHAF
                     ESAILKRLGEVELADQLEGIAELKRLPFVDGSRLGLSGWSYGGYMTLVAATRSPELWK
                     AAVAGAPVTHWKYYDSIYTERYMKLPGENAEKYESTAPLTHAAALGPKLLLLHGTADD
                     NVHMQQSIA"
BASE COUNT          832 a         1954 c         1696 g          671 t
ORIGIN      
        1 cgagatgcgg gccgaggagt cgaagatgcg cgcggcctgg cgccgcggcg aggtcgacga
       61 cctcatgaag cccgagaaca agaccgcgcg ctacaaggag ctcgaggcga agttcgacgc
      121 gctcgtcaag gagcagcgcg acctcctcgt caagaacgag ttcgaccgga tctacacgaa
      181 gaacggcgcc tcggggatga acgccttcac gtcgtacgac atgacggcct acttcatcac
      241 cgtgccgaag aacaagctcg agctctggtt ctggatggag tccgaccggc tgaaggaccc
      301 cgtcttccgc gagttctacg ccgagcgcga cgtcgtctac gaggagcgcc ggctgcgggt
      361 cgagtcgacc ccgaccggga agctcgacga ggcgttcgac tcgatcttct ggcactcgac
      421 cccctacacg tggcccgtca tcggctacgc ctccgacgtc gcgaacatca cgaagcagca
      481 ggccgacgac tacttcgcga cctactacgc cccgaacaac ctgacggccg tcctcgtcgg
      541 cgacttcgac aaggccgagg cgctgaagct cgcgaccgag tacttcggac ggatcccgcg
      601 cgggaagacg cccccgcccg aggtgaccac cctgccgacg aagtgggagt acgacctccg
      661 cttcgaggga gaggccgaca cgaacccgac cgccgagatc cggtggcgca ccgtcccctt
      721 ccagcacaag gactcgtacg ccctctcgat cctcgcggag ctcctgaacg gccggacggg
      781 acgcctctac aagtcgatgg tcctccccgc cgacgccgtc gccacgcagg cctacgccgt
      841 ccacaacggc ttcgtcggcc cggcgaagta cgccggctcg ttcgtgatcg gcgccgaggc
      901 gaaggagggg cagctcccgc ccgaggtcgc cgcgaaggcg ctcgccgaga tcgagcgcct
      961 caagaaggag cccgtccccg ccgacgagct ccggaaggtg aagaacaacg tcgcggcgaa
     1021 ctccttccgc cgcctctcct cgaacttccc gatcctcctc cagctgatgg tcaacgaagc
     1081 ctatggcgac tggacggaga tcaactccgg cccgaagaag ctcgaggcgg tcacggccga
     1141 ggacgtggcg cgggtcgcga agacgtacct cgtgaaggag aaccaggcga cggccctcgt
     1201 ctcgcgcaag gcgggctcgg ccccctcggc cgacgacgcc gccctcgccg aggtcccgga
     1261 gcaggtccgc ccgatgatca agcaggcgct ccagcagctc gccgccgaga aggacgccgc
     1321 gaagctgaag gaaggcctcg cgcagatgga gacgcaggcg gcgcaggtgc cgccccagat
     1381 gaagaagggc ttcgagctcc tcgtcacgaa ggcgcgcgag cggctcgccg agctcgagaa
     1441 ggccggggac aagaagtgag gagccggacg gtgaagacga tgaagaacct cgcgcggacc
     1501 tccgccctcg cgctcctcgg cgccctcctc ctcgcccccg cggcgcaggg cgtgccgccc
     1561 caccccgaca agctgaccta cccgaagttc acctaccagc cgccgtcggc gaaggagtac
     1621 cggaccgtcc tgaagtcggg cccggtcgcc ttcgtcgccg aggaccgcga gctgccgctc
     1681 gtctcgatca acgtcgccct ccggggcggc acctacctca gccccgccgg gaaggaaggg
     1741 ctcgccgacc tcgccggcta cctcctcgcg cgcggcggga ccgccacgaa gacggccgag
     1801 gagctcgagg agcggctcgc tttcctcgcc gcgtccctga actcgggcta cggccccgac
     1861 cgcggcaacg tctcgctcaa cctcctcgcc aaggacctcg acgagggcct cgccatcctc
     1921 cgcgaggtgc tcacggcgcc ccgcttccag gacgacaagc tgaagctccg ccgcgaccag
     1981 ctcctgaacg acatgaagtc gcgcaacgac gacacggccg acatcgagca gcgcgagcgc
     2041 ggcttcctcg ccttcggcga ggggtactac acgaaccgct acacgacgaa ggcctccctc
     2101 gagtcgatca cccgcgagga cctcctcgcc ttccaccgca ggtgggtcga cccgaggaac
     2161 gtgaccgtcg ccgtcgccgg cgacttcggc aagggtgtct cccgcgcgca gatgatcgcg
     2221 aagctcgacg ccctcttcgc gaagtggccc tacaagggag aggcggcgcc tccggtcccg
     2281 acgcccaccc acgccatggc ccccggcctc tacgtcgtcg acaaggacgt caaccagggg
     2341 cgcgtctccg tgatgctccc ggcgctcgcg cggaccgacc ccgactacga cgccgcgctc
     2401 gtcatgaacg acgtcctcgg cggcgggggc ttcacgagcc gcatcacgaa ccgggtccgc
     2461 tccgacgagg ggctcgccta ctcggccggg tccgggatcg tcgggggcgt ctggtacccc
     2521 gcgacgttcc gggcctcgtt ccagtcgaag gcccgcacgt gcgcctacgc gacgcagatc
     2581 gtcctcgagg agatgacgaa ggtccgcgac gccgaggtga ccgacgagga gctccagacc
     2641 gcgaagagct cgttcatcga caccctctcc cgcaacttcg ccacgaaggc gcagacggtc
     2701 aacgtcttcc tcgacgagga gttcaccggg cgattcgcga aggacccgga ctactacagg
     2761 aactacgccg cccggatcga gaaggtgacg aaggccgacg tgcagcgcgt cgcccggcgc
     2821 ctcctcgcaa cggacaaggt cgccgtcctc gccgtcggca agaaggacga cctcctgaac
     2881 cccgacccga agcacccggt gaagttcccc gagctgacgg gcggaaagct gaccgacctg
     2941 ccgctccggg accccttcac gatgaagccc ctcgcccccg cggcgaagta gctccacgca
     3001 cgtccccgcg ggcgacggcc cgcgccccgc gccccccggc accccggggg gcgcttctct
     3061 tttcggctat cgtcccggac gatgcgcgcg acgctccccg tcctcccggc cgccctcctc
     3121 gccctcttcc tcgggtcccc cggcgccgtc ctcggcggac cgccggccgg aagggccgcg
     3181 ctgacgctcg agcgggtcca cgccgccgag ccgctcgtcg agcccgcgcc gaccggcctg
     3241 cgctggcgcg acgcgagccg cttcacgtac ctgaagcgcg agagctccga gccgggcgcg
     3301 cgcgcctcgc tctggcagga ggacgccgcc acggggaagg ccgggaagct cctcgacgcg
     3361 attcccgccg agggaacggg gaaggacggc acgccgcggt cgctcccgct ctcggacgcg
     3421 agctggaacg aggcggggac cgccctcgtg gtcgtcgccg agaacgacct ctggctctac
     3481 gcgttcggcg ccgggccggc gcggcgcctg acgtccgacg ccgacgccga ggagctcccc
     3541 gtcttctccc cggacggcac gaaggtcgca ttcgtgaagg ggaacgacct ctggttcgtc
     3601 gaggtgggca ccggccgggc gacccgcctg acgaccgacg gcagcgcaac cgtgctcaac
     3661 gggcggctcg actgggtcta cgaggaggag ctcgccggcc ggaacggcgg gagggcctac
     3721 gaatgggccc cggactcctc ggccatcgca ttcctgcgcc tcgacgtctc gaaggtcccc
     3781 gagtatccgc tcgtcgactt cctcccgacg aacgggaagc tcctcccgca gcgctacccg
     3841 aagccgggcg atccgaacgc gtctccctcg gtgagggtcg tgacctggga ctcctccggc
     3901 ctcgcgacca cgagccggtc cgtctcctac gacggggagc gcgacctcct cggccccgag
     3961 ctcgcctgga ctcccgactc ctccgcggtc gcgttcacgc ggatggaccg gacgcagacg
     4021 gcgatcgagg ccttcctcct cgaccggtcc ggcagcgccg gccctcggag cctccttcgc
     4081 gagtcgtcgg cctcctgggt caacgcgcac gagccgccga tcttcctccc cgacggctcc
     4141 gggttcctct ggctctcgga gcggagcggc ttcctccacc tctaccgata cgggagggac
     4201 gggacgcttc tcgggccggt cacccgcggg gagtgggcga tcacggacac cccgcgcctc
     4261 gacgcgcggg cgggcagcgt cacgttcacg gcgaccgagg ccgacccgcg cgagcgccac
     4321 ctctaccgcg tccgcctgga cgggaccggc ctcacccggc tcacctccgg gaaggggacg
     4381 cacgccgcga tcccctcccc ggacggccgc tgggtcgtcg actcctggtc ggccgtcgac
     4441 acgccgacgc gcgtcgacct cgcgcggggg gacgggacgc ccctccggcc cgtcttcgtc
     4501 ccgaagacgt ccctcggcga cttcgcgctc gcgacgacgg agatgggctc cttccgcggc
     4561 tccgacggga cgctcttcta cacgcgcctc gtcaagccgg ccggcttcga cccggcgagg
     4621 aagtacccgg tcgtcgtcta cgtctacggc ggaccgcacg cccaggtcgt ccaggagcgg
     4681 tggggggcga cctcgctcct cgaccacgtc ctcgccgcga acgggttcct ggtctgggcg
     4741 atggacgggc gcggctcggg cggccgggga cacgccttcg agtcggcgat cctgaagcgc
     4801 ctcggcgagg tggagctcgc ggaccagctc gaggggatcg cggagctgaa gaggctcccg
     4861 ttcgtcgacg ggtcccgcct cggcctctcc ggctggtcct acggcggcta catgacgctc
     4921 gtcgccgcga cgcgctcgcc ggagctctgg aaggccgcgg tcgccggggc tcccgtgacc
     4981 cactggaagt actacgactc gatctacacc gagcgctaca tgaagctccc gggggagaac
     5041 gcggagaagt acgagtcgac ggcgcccctg acccacgccg cggccctcgg gccgaagctc
     5101 ctcctcctcc acgggacggc ggacgacaac gtccacatgc agcagtcgat cgc
//
LOCUS       DAOTEZ010000319         5125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_11751_length_5125_cov_6.899408, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000319 DAOTEZ010000000
VERSION     DAOTEZ010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5125
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11751_length_5125_cov_6.899408"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..157)
                     /locus_tag="P5164_14790"
     CDS             complement(<1..157)
                     /locus_tag="P5164_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HRY45209.1"
                     /translation="MLTGEPRNLIDSVWNAFAAGGIANPLEVIEQITYLLFLRRLDDL
                     QTLAENKA"
     gene            322..2619
                     /gene="ccsA"
                     /locus_tag="P5164_14795"
     CDS             322..2619
                     /gene="ccsA"
                     /locus_tag="P5164_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRY45210.1"
                     /translation="MKKLVETLSSLKLAVGLLVVLLIGLSIGTILESRTDAATAQRVV
                     YYSWWFLALQGLFALNVACSLANLFPWGKKRAGFVTTHAALLLILAGATLTYFWKVEG
                     QLGLWEGENGATIENHDAQGNVVSRHDLPFSVKLVDFVLDTYPGTMRPAGFASEVVIT
                     DTDTGKSFPARIWMNHPLHHRGYTLFQSSYQQQGGREATVLSVSKDPGQLVAFAGYVA
                     VVLGMILVLVTRVSQARERDRERAALEAKVAQGGMSLPTMGKALLLGLALLPLAAPAA
                     TPELEALRRLPVQHDGRTMPLDTLARETVWNVTGSHAFRGEDPAATFTGWLFDPRAAA
                     TTPVVKVGSKALAAAAGLPPGTKYVSFEQLVGNQRLMGLIRQAGMAAQREEPRRGVLK
                     DAEKLENRLNVLYGLLQRETVRPIPASDAKARWNRPANVTKEALLALANGPRLPGWPT
                     AKQVDREILYNRVNPAKLAWIVLLASLVVAIVAWSKASKALDWTSFALLAIGFGVMTW
                     GIGMRWAAGERIPAANMYESLLFLAWGVGLFAVIAYLLMRNRTVVLNAAAMSALTMAL
                     TDLLPMDRFIHPIAPVLAGTPWLAIHVPIIMVGYAVLALGVVVAHMQLAFAAFGKGKG
                     ALVERLYELLYWYMHVGSILLLAGILTGSVWAASSWGRYWGWDPKEVWSLVAFLAYMA
                     ILHLKVDKVIGTFGAAAISILAFQTILMTYLGVNYVLNVGMHSYGMGDSPIVKWMVLV
                     GLAEVVFLAVAGVAYRDRKRLLAEA"
     gene            complement(2680..3261)
                     /locus_tag="P5164_14800"
     CDS             complement(2680..3261)
                     /locus_tag="P5164_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45211.1"
                     /translation="MKSPAPRIRKVLFAVGLVSLFAFSHPLGAEEPKKAETLPSPASA
                     PAGSGAAALEKLKALAGDWVEIEPKVGEKGAVAVMYRVTGAGSAVVSTLAPGTPHEMV
                     SVWHRDGNDLVMTHYCAAQNQPRFRTKAVPANVVAMEFDGGTNVDPAKDVHIHAVKVE
                     LLGPDEIRETWIGWKGGRTEEPPLVLHLTRKKG"
     gene            complement(3303..4637)
                     /locus_tag="P5164_14805"
     CDS             complement(3303..4637)
                     /locus_tag="P5164_14805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRY45212.1"
                     /translation="MNRTGMVPDEAGPGASVLRAFDVVVCGGGPGGSTAATLLARAGY
                     SVALFERERFPRFHIGESLLPWNVPLFRRLGVLEKLEATGPQVKYGARFYHQGTDHAR
                     LVRFANAYDGAPASAFQVKRAEFDALLLDHARASGVAVREQARVEEVLFEGERAVGVR
                     VKVEGDAPCDVKAKAVVDATGRDALMSRRLGGRRKDPELDRSAAFAHFSGFRRAEGPT
                     GGDIVVVTTADGWWWLIPFSDGTVSVGVVMPSSRFAKRPGTVEELFEARVAATPEVRD
                     LLEGNRRLGEVRAIPDYSYRTPRISGDGFCLVGDAACFLDPVFSSGVLLAMSSGEMAA
                     EAIGRALSRRGRVDAADFRTMERRYGRAIARFSRFVHGFYTPHVLETFYTESPVPLIT
                     GAVTTVLAGAVFRPSLKSRLGASAFHLASLLVRLKQRASGAGPFERATGLIE"
     gene            4827..>5125
                     /locus_tag="P5164_14810"
     CDS             4827..>5125
                     /locus_tag="P5164_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004556311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRY45213.1"
                     /translation="MSDYRIQEHPILAIPGEAVVPFTWKGEALKARPGETIASALFAN
                     GVRIFGHHHKDGSPQGIFCANGQCAQCSVVANGLSVKSCMVPVTEGMAVEPLDGKA"
BASE COUNT          739 a         1783 c         1841 g          762 t
ORIGIN      
        1 gggccttgtt ctcggcgagg gtctggaggt cgtcgaggcg gcggaggaag aggagatagg
       61 tgatctgctc gatgacctcg agcgggtttg cgatgcctcc cgcggcgaag gcgttccaaa
      121 ccgagtcgat caggttgcga ggttcgccgg tgagcacgtt ccgtccccct gcgcggcccg
      181 acggcggaga ggccgaacgg gcgattggaa ggccgaggcg agtctacccg gccccgcctc
      241 gacacccctt ctccgaaatg ggactattca tggcctttta gggcctgccg ctcgagcggc
      301 gggccccagg acggagcccc cttgaagaag ctcgtggaaa ccctctcctc cctcaagctc
      361 gccgtcggcc tcctcgtcgt cctgctgatc gggctgtcga tcgggacgat cctcgagtcg
      421 cggaccgacg cggcgacggc gcagcgggtc gtctactact cgtggtggtt cctcgccttg
      481 caggggctct tcgcgctgaa cgtcgcctgc tcgctcgcga acctcttccc gtgggggaag
      541 aagcgggccg gcttcgtgac gacgcacgcg gcgctcctcc tcatcctggc gggggcgacg
      601 ctcacctact tctggaaggt ggaggggcag ctcggcctct gggaaggtga gaacggcgcg
      661 acgatcgaga accacgacgc gcaggggaac gtcgtctccc ggcacgacct gccgttctcg
      721 gtgaagctcg tcgacttcgt cctcgacacc tacccgggga cgatgcggcc ggccggattc
      781 gcgagcgagg tcgtcatcac cgacaccgac accggaaagt cgttcccggc ccggatctgg
      841 atgaaccacc cgctgcacca ccgcgggtac acgctcttcc agtcgagcta ccagcagcag
      901 ggggggcgcg aggcgacggt gctctcggtc tcgaaggacc cggggcagct cgtcgccttc
      961 gccggatacg tcgcggtcgt cctcgggatg atcctggtcc tcgtgacgcg cgtctcccag
     1021 gcgcgggagc gggaccgcga gagggccgcg ctcgaagcga aggtggcgca gggcgggatg
     1081 tcgctcccga cgatggggaa ggcgctcctc ctcggcctcg cgctcctgcc gctcgcggcg
     1141 ccggcggcga cgccggagct cgaggcgctc cgccggctcc cggtgcagca cgacgggcgg
     1201 acgatgccgc tcgacacgct cgcgcgcgag acggtctgga acgtgacggg gtcgcacgcc
     1261 ttccgcggcg aggacccggc ggcgacgttc accggctggc tcttcgaccc gcgcgcggcc
     1321 gcgacgacgc cggtcgtgaa ggtcggcagc aaggcgctcg cggcggcggc ggggctcccg
     1381 ccggggacga agtacgtctc gttcgagcag ctcgtcggga accagcgcct catggggctg
     1441 atccgccagg cggggatggc cgcgcagcgg gaggagccgc gccggggcgt cctgaaggac
     1501 gccgagaagc tcgagaaccg gctgaacgtc ctctacgggc tcctccagcg ggagaccgtc
     1561 cggccgatcc cggcgagcga cgcgaaggct cgctggaacc gcccggcgaa cgtgacgaag
     1621 gaagcgctcc tcgccctcgc gaacggcccg cgcctgccgg gctggccgac ggcgaagcag
     1681 gtcgaccggg agatcctcta caaccgcgtc aaccctgcga agctcgcctg gatcgtcctc
     1741 ctcgcctcgc tcgtcgtcgc gatcgtcgcc tggtcgaagg cgagcaaggc gctcgactgg
     1801 acctcgttcg cgctcctcgc catcggcttc ggcgtgatga cctgggggat cgggatgcgg
     1861 tgggccgcgg gcgagcggat cccggcggcg aacatgtacg agtcgctcct cttcctcgcc
     1921 tggggcgtcg gcctcttcgc ggtgatcgcc tacctgctga tgcggaaccg gaccgtcgtc
     1981 ctgaacgccg cggcgatgtc ggcgctcacg atggcgctca ccgacctcct cccgatggac
     2041 cgcttcatcc acccgatcgc gcccgtcctg gccgggacgc cctggctcgc gatccacgtc
     2101 ccgatcatca tggtcggcta cgcggtcctc gcgctcggcg tcgtcgtcgc gcacatgcag
     2161 ctcgcgttcg cggcgttcgg gaaggggaag ggggcgctcg tcgagaggct ctacgagctc
     2221 ctctactggt acatgcacgt cggctcgatc ctccttctcg cggggatcct gacgggctcg
     2281 gtctgggccg cctcgtcgtg gggccgctac tggggatggg acccgaagga ggtctggtcg
     2341 ctcgtcgcgt tcctcgccta catggcgatc ctccacctga aggtcgacaa ggtgatcggg
     2401 acgttcggcg ccgcggccat cagcatcctc gccttccaga cgatcctgat gacctacctc
     2461 ggcgtgaact acgtcctgaa cgtcggcatg cactcctacg gcatgggcga ttcgcccatc
     2521 gtgaagtgga tggtcctcgt cgggctcgcc gaggtcgtct tcctcgccgt cgccggcgtc
     2581 gcgtaccgcg accgcaagag gctgctcgcg gaggcctgag gacgggaagc ccgagcggag
     2641 cgccgcggcc cgggagggcg cggcgctccg gcgttccggt cagcccttct tccgcgtcag
     2701 gtggaggacg agcggcggct cctcggtcct gccgcccttc cagccgatcc aggtctcgcg
     2761 gatctcgtcc gggccgagga gctcgacctt cacggcgtgg atgtgcacgt ccttcgccgg
     2821 gtcgacgttc gtgccgccgt cgaactccat cgcgacgacg ttcgccggca ccgccttcgt
     2881 ccggaaccgc ggctggttct gcgcggcgca gtagtgggtc atcacgaggt cgttcccgtc
     2941 gcggtgccag acggagacca tctcgtgggg cgtgccgggg gcgagcgtcg agacgacggc
     3001 cgacccggcg cccgtgaccc ggtacatgac ggcgacggcc cccttctcac cgaccttcgg
     3061 ctcgatttcg acccagtcgc ccgcgagggc cttcagcttc tcgagggcgg cggcgccgga
     3121 tccggcgggg gcggacgcgg gagaggggag cgtctcggcc ttcttcggct cctcggcgcc
     3181 gaggggatga gagaacgcga agagggagac gaggccgacg gcgaagagaa cctttcgaat
     3241 gcggggcgcg ggcgacttca tggagacctc cttcccgatg cggcgggatc gtacctgcgg
     3301 cgctactcga tgagtccggt cgctctctcg aaggggccgg cccccgacgc cctctgcttc
     3361 agccggacga ggagcgaggc gaggtggaac gcggacgctc cgagcctcga cttcaggctc
     3421 gggcggaaga cggctcccgc gaggacggtc gtcacggccc ccgtgatcag ggggaccggc
     3481 gactcggtgt agaaggtctc gaggacgtgc ggcgtgtaga agccgtggac gaaccggctg
     3541 aagcgggcga tcgcccggcc gtagcgcctc tccatcgtgc ggaagtccgc cgcgtcgacc
     3601 cgcccgcggc gcgagagcgc gcggccgatc gcctccgcgg ccatctcccc ggaggacatc
     3661 gcgaggagga cgccgctcga gaagaccggg tcgaggaagc aggccgcgtc gccgacgagg
     3721 cagaagccgt ctcccgagat gcggggcgtc ctgtaggagt agtccgggat ggcgcggacc
     3781 tcgccgagcc gacggttccc ctcgaggaga tcgcggacct cgggcgtcgc ggcgacccgc
     3841 gcctcgaaga gctcctcgac cgttcccggc ctcttcgcga agcgggacga cggcatgacg
     3901 acgccgaccg agacggttcc gtcggagaac gggatgagcc accaccagcc gtcggccgtc
     3961 gtcacgacga cgatgtcgcc tccggtcggc ccctccgccc ggcggaagcc ggagaagtgg
     4021 gcgaacgcgg ctgatcggtc gagctccggg tccttccggc gtccgccgag ccggcgcgac
     4081 atgagggcgt cccgccccgt cgcgtcgacg accgctttcg ccttcacgtc gcagggcgcg
     4141 tcgccttcga ccttcacgcg gaccccgacc gccctctcgc cctcgaagag gacctcctcg
     4201 acccgcgcct gctcgcggac cgcgacgccc gaggcgcggg cgtgatcgag gagaagcgcg
     4261 tcgaactccg cccgcttcac ctggaaggcc gaggccggtg cgccgtcgta ggcgttggcg
     4321 aaacggacca ggcgcgcgtg atcggtcccc tggtggtaga agcgggcgcc gtacttcacc
     4381 tgcggcccgg tcgcctccag cttctcgagc actccgagcc tccggaagag cgggacgttc
     4441 cacggcagga gcgactcgcc gatgtggaag cgcgggaacc tctcccgctc gaagagcgcg
     4501 acggagtagc cggcgcgcgc gaggagcgtc gccgcggtcg agccgcccgg tcctccgccg
     4561 cagacgacga cgtcgaaggc ccgcagaacg gacgcgccgg ggcccgcctc atctgggacc
     4621 atgccagtcc tgttcattcg cggatcatag agagaggacg ggcgaaatcg gggaggaaac
     4681 gacgggagag gagctcctcc tctctggacg cagcgccccg tcctccttat cctcgcctgt
     4741 tgggccgccc ccgcacgggc agcggccgat ccccttcccg gcgaccgcgg tcccgcggcg
     4801 ccggcgagcc ggggacgagg agcctcatgt cggactacag gatccaggaa cacccgatcc
     4861 tcgcgatccc gggcgaggcg gtcgtgccgt tcacgtggaa gggcgaggcg ctgaaggcgc
     4921 ggccgggcga gacgatcgcg tcggcccttt tcgcgaacgg ggtgcgcatc ttcgggcacc
     4981 accacaagga cggctcgccg caggggatct tctgcgcgaa cggccagtgc gcccagtgct
     5041 cggtcgtggc gaacggcctc tccgtcaagt cgtgcatggt gccggtgacg gaggggatgg
     5101 cggtcgagcc gctcgacggc aaggc
//
LOCUS       DAOTEZ010000320         5117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_11780_length_5117_cov_6.360727, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000320 DAOTEZ010000000
VERSION     DAOTEZ010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11780_length_5117_cov_6.360727"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="P5164_14815"
     CDS             complement(<1..1066)
                     /locus_tag="P5164_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45214.1"
                     /translation="MIAFITLFLGIATGVHTVELSAASDVARVELYVDGTKSADVGPP
                     FTAKIDLGPEIAPRELVAVAFDAGGRRLGEARQQVNRATSDAEAGFALERDRTGHVLA
                     ARLFWRCPVSPKPTSISASFDGQPLEVANPERIPIPSHGVGSEHILVADLTFDGGITA
                     TAVASFGGRNRGDETKELTALPVRLSPGARFPKKADRMDGWFQLEGRSLAVAAIEEGP
                     TEIVFVMAGRVPEDLMRLANDLVRADEDRREVERLTAGRIASQVGRPAGEHVAVPPLS
                     RRSPTSVLDLPTGSRFHFTRSTPRMIAGPREVTWAFPTSAVFTPSDGTFLHLGKHVFR
                     SEEPSPPRIAEAIAASGLAAT"
     gene            complement(1141..2994)
                     /locus_tag="P5164_14820"
     CDS             complement(1141..2994)
                     /locus_tag="P5164_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY45215.1"
                     /translation="MAGHLPEDEAQGRGFDRGLLRRLFTLALPYKGYAGGALLVLFLE
                     SAAQLAGPLLTGAAIDLVFTPRGEAAGGAAFVARLLASLGLPVSGLRGLDAIAGLYLL
                     SVLANFGFMFLMVEWTSRMGQGVMYDLRTRIFGHLQRADVALYDRTPVGRLMTRLTTD
                     VDALNELFTSGFVSLLGDLAVLFGIVGVLYALDPTLATITLAVLVPMALVTNWFRKGA
                     RDTYRKVRVRIAKVNSFLQEHLSGIAVVQLFRREAPAQDAFREVNDEHRQANLESIFY
                     YAVFYPALDFLSAVGMAAILWWGGGEVIRGTMTIGAVVTFLQYSKRFYRPLMDLSEKY
                     NILQAAMASSERIFQLLDETPAIAAPPSPRPVPRPLRGEIDFDGVRFSYVPGEEVLKG
                     VSFRAAPGETIALVGATGSGKSTIVSLLLRFYDVTGGAVRVDGHDVRSFAPTEFRDGF
                     GIVLQDTFLFTGTIAENVAFFDPSMPRETVECACRDVGLGPLLDRLPDGLDTRLTERG
                     GGLSVGEKQLVSFARALARDPRILILDEATSSVDPETEAVIEKAVDRLLSGRTSVVVA
                     HRLSTIVRADRILVLSHGEVRESGTHAELLAKGGLYARLYALQLKDREAAA"
     gene            complement(2997..4727)
                     /locus_tag="P5164_14825"
     CDS             complement(2997..4727)
                     /locus_tag="P5164_14825"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY45216.1"
                     /translation="MKDSARFLSYFARRPGAYAAGFGTMALSAALFLAMPRIVRYVLE
                     SLETDPSPRRIALACGAILLLGAADAVLFFFTRRILIGASRDVEFEMREDLFAHLLRL
                     PPRWHRKSRVGDVMSRAVNDLSAVRMMIGPGIMQAANTLVVGSIALTLMAFASPSLTL
                     VALGVLPFVVVATQGMGKVTHRRFTAIQEFFSEISASAQENFNGARLVRAFAREATEE
                     ARFDAKNRELRRRNLRLARLNALYFPLLQALVGSGFAIILWVGGRQILAGELTVARYV
                     EFNLYLMELVWPAIALGWVVNLWQRGTASWNRMAELWAAEPLPEESGEGPLPNGPLEA
                     KGLTFGYDGGPPVLRDVAFTVEPGTTVAVVGRTGSGKSTLLQLLLRLEEPPFGTIRLG
                     GRDLREVPLPLLRRTLVAVPQETFLFSDTLAANVAMGRPGATRAEVERAALEAGLGPD
                     LATFPKGLDTIVGERGITLSGGQKQRTALARALLADAPFLVLDDAFSAVDTETEEKIL
                     EAVKARSGTRTIFLVSHRLSTIQHADFVVVLEKGRVVETGTHEELLRAGGLYAELAER
                     QRLSEEVEAA"
     gene            4809..>5117
                     /locus_tag="P5164_14830"
     CDS             4809..>5117
                     /locus_tag="P5164_14830"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRY45217.1"
                     /translation="MTTALLERIPLFRCLNPAEAKALAQVCRLQVCERGDTVFVEGSR
                     AKEMSFVALGTVKIVKATPTRSVILRVVGAGSPVGIVAAWEGRPYPGTAIAVEPSTVV
                     H"
BASE COUNT          796 a         1676 c         1952 g          693 t
ORIGIN      
        1 gggttgccgc gagcccggag gcggcgatcg cctcggcgat ccgcggcggt gaaggctctt
       61 cgctgcggaa gacgtgcttc ccgagatgca ggaacgtacc gtcactgggc gtaaagaccg
      121 cggaggtggg gaaggcccac gtaacctctc gcggacccgc gatcatccgc ggggtcgagc
      181 gtgtgaagtg gaaccggctc cccgtcggca ggtccagcac cgacgtcggg gacctgcggc
      241 tgagtggggg gactgcgacg tgttcgcccg cgggacgccc gacctgagag gcgatccggc
      301 cggcggtaag ccgctcgacc tctctccggt cttcgtccgc ccggacgagg tcgttggcga
      361 ggcgcatcag gtcctccgga acccggcccg ccatgacgaa cacgatctcg gtcgggccct
      421 cctcgatggc cgccaccgcc aggctccgtc cttccagctg aaaccagccg tccattcgat
      481 ccgccttctt cgggaaccgg gcgccgggtg agaggcggac ggggagggct gtgagctcct
      541 tcgtctcgtc gccacggttc ctccctccga agctcgccac cgccgtcgcg gtgatgcctc
      601 cgtcgaaggt caggtcggcg acgaggatgt gctcggagcc cacgccatgg gaagggatcg
      661 ggatccgctc ggggttcgcc acctcgagcg gctgaccgtc gaaggacgcg gagatcgacg
      721 tcggcttcgg gctcacgggg cagcgccaga agaggcgagc ggccagaacg tggcccgtcc
      781 tgtcgcgctc gagcgcgaag ccagcctcgg cgtcggaggt tgcgcggttg acctgctgtc
      841 tcgcttcacc gagccgtctc ccgcctgcgt cgaacgcaac ggcgacgagc tcccgcggtg
      901 cgatctcggg gcccaggtcg atcttcgccg tgaagggcgg gccgacgtcg gcagacttcg
      961 tcccgtcgac gtagagttcc acccgtgcga cgtcggaggc cgccgagagc tcgaccgtgt
     1021 gtacgcccgt cgcgatgccg aggaagaggg tgatgaacgc gatcaaagat tcctccgggc
     1081 gggggagcgc acgggtcgtg ccagcggcac cgtcccctcg cagggacgta cgctcgcctc
     1141 ctacgccgcc gcctcccggt ccttgagctg aagcgcgtag agacgggcgt agaggccccc
     1201 cttcgcgagg agctcggcgt gcgtcccgga ttcccggacc tcgccgtgcg agaggacgag
     1261 gatccggtca gccctcacga tcgtcgagag gcggtgggcg acgacgacgg aggtgcggcc
     1321 cgagaggagc cggtcgacgg ccttctcgat gacggcttcc gtctcggggt cgacgctgga
     1381 ggtcgcctcg tcgaggatca ggatgcgcgg gtcgcgggcg agggcccggg cgaaggagac
     1441 gagctgcttc tcgccgacgg agaggccgcc gccgcgctcc gtcagccggg tgtcgaggcc
     1501 gtcggggagg cggtcgagga gagggccgag gccgacgtcg cggcaggcgc actcgaccgt
     1561 ctcgcgcggc atcgacgggt cgaagaaggc cacgttctcg gcgatcgtcc cggtgaagag
     1621 gaacgtgtcc tgcaggacga tcccgaagcc gtcgcggaac tccgtcgggg cgaagctccg
     1681 cacgtcgtgc ccgtcgacgc gcacggcgcc gccggtgacg tcgtagaagc ggaggaggag
     1741 gctcacgatc gtcgactttc cggagccggt cgccccgacg agggcgatcg tctcgccggg
     1801 cgcggcgcgg aacgagaccc ccttgagcac ctcttccccc ggcacgtagg agaagcggac
     1861 gccgtcgaag tcgatctcgc cgcggagcgg gcgggggacg gggcggggag aaggcggagc
     1921 cgcgatggcc ggcgtctcgt cgaggagctg gaagatccgc tccgaggagg ccatcgccgc
     1981 ctggaggatg ttgtacttct cggagaggtc catgagcggc cggtagaacc gcttcgagta
     2041 ctgaagaaag gtgacgacgg cgccgatcgt catcgtcccg cggatgacct cgccgccgcc
     2101 ccaccagagg atcgccgcca tgccgacggc cgagaggaag tcgagggcgg ggtagaagac
     2161 cgcgtagtag aagatcgact cgaggttcgc ctgccggtgc tcgtcgttca cctcccggaa
     2221 ggcgtcctgc gcgggcgcct cgcgccggaa gagctggacg acggcgattc cggagaggtg
     2281 ctcctggagg aacgagttca ccttcgcgat cctgacgcgc accttccggt aggtgtcgcg
     2341 cgcccccttc cggaaccagt tcgtcacgag ggccatcggg acgaggacgg cgagtgtgat
     2401 cgtcgcgagc gtcgggtcga gggcgtagag gacgccgacg atcccgaaga ggacggcgag
     2461 gtccccgagg agcgagacga agcccgaggt gaagagctcg ttcagggcgt cgacgtccgt
     2521 cgtcaggcgc gtcatgaggc ggccgacggg cgtccggtcg tagagcgcga cgtcggcgcg
     2581 ctgaaggtgc ccgaagatcc gggtccggag gtcgtacatc accccctgcc ccatccgcga
     2641 cgtccactcg accatcagga acatgaagcc gaagttcgcg aggacggaga ggaggtagag
     2701 gcccgcgatc gcgtcgaggc cgcggagccc ggagaccggg aggccgagcg aagcgaggag
     2761 gcgcgcgacg aaggccgccc ctccggccgc ctcccctctc ggcgtgaaga cgaggtcgat
     2821 cgcggcgccc gtcaggagcg ggccggcgag ctgggcggcc gactcgagga agaggacgag
     2881 gagggcgccc cccgcatacc ccttgtacgg gagagcgagg gtgaagaggc gccgcaggag
     2941 gccgcggtcg aagccgcgtc cctgggcctc gtcctcgggg aggtggccgg ccatctttac
     3001 gcggcctcca cttcctcgga gaggcgctgg cgctcggcga gctccgcgta gaggccgccg
     3061 gcgcgcagga gctcctcgtg ggtccccgtc tcgacgacgc gccccttctc gaggacgacg
     3121 acgaagtcgg cgtgctggat cgtggagagg cggtgagaga cgaggaagat cgtcctcgtc
     3181 ccggagcggg ccttcacggc ctcgaggatc ttctcctccg tctcggtgtc gaccgcggag
     3241 aaggcgtcgt ccaggacgag gaacggggcg tcggcgagga gggcgcgggc gagggccgtc
     3301 cgctgcttct gtcctccgga gagcgtgatg ccgcgctcgc cgacgatcgt gtcgagcccc
     3361 ttcgggaacg tcgcgaggtc ggggccgagg ccggcctcca gggcggcccg ctcgacttcg
     3421 gcgcgcgtcg ccccggggcg tcccatcgcg acgttcgcgg cgagcgtgtc ggagaagagg
     3481 aacgtttcct gcgggacggc gacgagcgtt cggcgcagga gagggagcgg gacctcgcgc
     3541 aggtcgcgtc cgccgagccg gatcgtcccg aacggcggct cctcgaggcg gaggaggagc
     3601 tggaggagcg tcgattttcc cgagccggtc cggccgacga cggcgaccgt cgtccccggc
     3661 tcgaccgtga acgcgacgtc gcggaggacg ggcggcccgc cgtcgtagcc gaaggtcagc
     3721 cccttcgcct cgagcggccc gttcgggagc ggcccctcgc cgctctcctc ggggagcggc
     3781 tcggccgccc agagctcggc catccgattc caggaggcgg tcccgcgctg ccagaggttc
     3841 acgacccagc cgagggcgat cgcgggccag acgagctcca tcaggtagag gttgaactcg
     3901 acgtaccgtg cgaccgtcag ctcgcccgcg aggatctgcc ggccgccgac ccagaggatg
     3961 atggcgaatc cgctcccgac gagcgcctgc aggagcggga agtagagggc gttcagccgc
     4021 gcgaggcgga ggttccggcg gcgcagctcg cggttcttcg cgtcgaagcg ggcctcctcg
     4081 gtcgcctccc gggcgaaggc gcggacgagg cgggccccgt tgaagttctc ctgcgcgctc
     4141 gcggagatct cgctgaagaa ctcctggatc gccgtgaaac ggcggtgcgt gaccttgccc
     4201 atcccctgcg tggcgacgac gacgaacggg aggacgccga gggcgacgag cgtcagggag
     4261 ggggaggcga aggccatgag ggtcagcgcg atcgacccga cgacgagggt gttcgccgcc
     4321 tgcatgatcc ccggaccgat catcatccgg acggcggaga ggtcgttcac ggcgcggctc
     4381 atcacgtcgc cgacgcggct cttccggtgc cagcgcggcg ggagccggag gaggtgggcg
     4441 aagaggtcct cccgcatctc gaactcgacg tcgcgcgaag cgccgatgag gatgcggcgg
     4501 gtgaagaaga agaggacggc gtcggcggcg ccgaggagga ggatcgcccc gcaggcgagg
     4561 gcgatccttc gcggcgaggg gtccgtctcg agcgactcca ggacgtaccg gacgatccgc
     4621 ggcatggcga ggaagagagc cgcggagagc gccatcgtcc cgaaaccggc ggcgtaggcc
     4681 ccgggccttc gggcgaagta ggaaaggaag cgggccgagt ccttcaagga ggggcgatgt
     4741 tacccgtccc ggtccgcggc gcccccgggg atctccgggc cggctgatat cctcgccgtc
     4801 cacgcatcgt gacgaccgca ctgctcgaac ggattcccct gttccggtgc ctgaacccgg
     4861 cggaagcgaa ggcgctcgcg caggtgtgcc gtctccaggt ctgcgagcgg ggggacacgg
     4921 tcttcgtcga ggggtcgcga gcgaaggaga tgtcgttcgt cgccctcggc acggtgaaga
     4981 tcgtcaaggc gaccccgacc cgcagcgtca tcctccgggt ggtcggagcc gggagcccgg
     5041 tcgggatcgt cgcggcgtgg gaggggcggc cctacccggg gacggcgatc gccgtggagc
     5101 cctcgaccgt cgtccac
//
LOCUS       DAOTEZ010000321         5108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_11807_length_5108_cov_3.729072, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000321 DAOTEZ010000000
VERSION     DAOTEZ010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11807_length_5108_cov_3.729072"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="P5164_14835"
     CDS             <1..344
                     /locus_tag="P5164_14835"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY45218.1"
                     /translation="AFSSVAGDRGRRKNYAYGATKAALSTAMEGLSARLAPNGVTVLC
                     VKPGPADSAMTWGQFPASSPLLARPETVARDVLSALSRGRAVVYTPWYWRPIMAVLGA
                     LPAAVFRRLPI"
     gene            344..4333
                     /locus_tag="P5164_14840"
     CDS             344..4333
                     /locus_tag="P5164_14840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HRY45219.1"
                     /translation="MTENRSRRFEILLALLFAGAIAFRLFGLNWDQGHNYHPDERRIV
                     EAVQQLSISPLQLDPKFYAYGSLPFYVTRGTSSLLGNVSPWFGSWDGILLTGRALSAL
                     WGAAGCVLLALLGRKLFGERTGLLAVAFLAIAVFHVQNSHFATNDVPLTTLVLATFLL
                     LARALELRSVPAFLLAGGAAGLALAAKASAATLLLPLAAAPLLAFLPARRFGRGALAL
                     GAAALGLGAAFLLGQPSALTSSRELLAAVAEQGAMVRDAGSVPYTNQYVGTTYLLYEL
                     SEIVLWGLGPALGLAALFGVALRLRETARERERRELLLWLWAVPFLLLTASFEVRFPR
                     YLLPLHPLLLLGAAALLAPRAGEGSGRRFARRAVLGLSGAYLLAFLSIYTRPHSVVSA
                     SRWFHENAAEGATLLVPHWEEGFPFALDGLPAGRFRTADLPLYDPDGAEKTRLMAERL
                     ASADLLVFPTKRHTGAIPRARERYPDTDRFLRLLYSGDLGWKLVRTFSSPPQLFGLRL
                     PTELADESFSVYDHPRVTLFRNAARLDAAALERLLREATPSRVLTRREILRARPDDPH
                     ADSEERIRGTRSTPLALLLWLALLEALGLAAWRLLGPRLPAVPGVYALSKVAGVALFG
                     LAAWALVAWGLFAFVPSFALAAAVAVVLAGALSSRPLPALPRRELLATEGLFLGVFLF
                     FAAVRAFSPEIYWGEKPMDFSFLNALLRSELLPPPEPWLAGTTLSYTYFGHFLVAAFA
                     KALGVHPALAFNLGLAATAALTAAALLAAGAFLGGRLRTGLLAAGLGLFAGNLAGLLE
                     YARRRVVDFDLFWSVSRVLKTQNEINEFPFWSFLFADLHAHLLAFPFLLGLLAVLLLA
                     FRQRTSPELYVPPFGRSLLLALASLFLAVLLVTNGWSAPTAAGLLALLLSASALAATP
                     RPAPPLPARRLGRLLAGVVVPGLAVALPALLVTLPFWRAYTPPPRHLGWERGPGYDAL
                     SLLLVHGLGLALVVPFLLARLRETRHDPARPRRAAALAGGLALLLSLSLVDLPALATG
                     RLAPAPSVRAFLAGLALLALLLAFGRGLPPARRALPAVAAAAFGLLTLCEVVFVWDRM
                     NTVFKFGLDATLLLAVAGAGALDLLLERSPRPAPSRRLAATLAAAALLGALATSLLAL
                     GGHLRTRRVETPRGTLDGLAFLRSHRPGEAAALDFLEMRVPGLPTIVEAWGPSYREYA
                     RFASRSGLPTVLGWDYHLLQRGQPRPELDRRILDVARLYDPLSLYDLPEILSRREVRW
                     VVSGAEEVEAHGETHRALFRAFPGLLVPAFESGGVTLYRVPLRGRYETEHLVSAKPSR
                     RADPAKPND"
     gene            4431..5084
                     /locus_tag="P5164_14845"
     CDS             4431..5084
                     /locus_tag="P5164_14845"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRY45220.1"
                     /translation="MPTGRWAPPGKRRRGFSLIELLVVVVIIGISLTLGLSGILSALK
                     RQRLATAASEVNNLVTKVYSTMQDENLRTFLVFGKYVEGSGTDVAVVVDRNDNGVCDE
                     AIDPNNDGLLHDAPTNLVPWRVRVPEDVALSNVAANGQVFTGAWLRPSGSGPVSAVLL
                     CDFMGRAITPPEAVGGTPAVATGLATVQLSHRDMISGSLSPLVTYTVSIGPLFKSLN"
BASE COUNT          512 a         2216 c         1518 g          862 t
ORIGIN      
        1 gcgcgttctc ctcggtcgcg ggagaccgcg gccgccggaa gaactacgcc tacggcgcca
       61 cgaaggccgc gctctcgacc gcgatggaag gtctgtcggc ccgtctcgcc ccgaacggcg
      121 tcacggtcct ctgcgtcaag cccggaccgg ccgacagcgc gatgacctgg ggacagtttc
      181 cggcttcgtc gccgctcctc gcccggccgg agacggtggc gcgagacgtc ctctcggcgc
      241 tctcccgcgg acgcgcggtg gtctacaccc cctggtactg gcgcccgatc atggcggtcc
      301 tgggggccct cccggctgcc gtcttccgga ggcttccgat ctgatgacag agaaccgctc
      361 gcgccgattc gagatcctcc tcgcgctcct cttcgcgggg gcgatcgcgt tccggctctt
      421 cggcctcaac tgggaccagg ggcacaacta ccacccggac gaacgacgga tcgtcgaggc
      481 cgtccagcag ctttccatct ctcccctgca gctcgacccg aagttctacg cctacgggtc
      541 gctccccttc tacgtgacgc gcgggacgag ttccctcctc gggaacgtca gcccgtggtt
      601 cggctcctgg gacgggatcc tcctgaccgg ccgggccctc tcggctctct ggggcgccgc
      661 cggctgcgtt ctcctcgccc tcctcggacg gaagctcttc ggcgagcgga cggggcttct
      721 cgccgtcgcc ttcctcgcga tcgccgtctt ccacgtccag aactcgcact tcgcgacgaa
      781 cgacgtcccg ctcacgacgc tcgtcctcgc gacgttcctt ctcctcgccc gcgcgctcga
      841 gctgaggagc gtcccggcct tcctcctcgc cggcggcgcc gccggcctcg cgctcgcggc
      901 gaaggcgagc gccgcgacgc tcctcctccc gctcgcggcc gcgcccctcc tcgcgttcct
      961 cccggcacgc cgcttcgggc gcggcgccct cgccctcggg gccgccgcgc tcggcctcgg
     1021 cgcagctttc ctcctcgggc agccctcggc gctcacctcc tcgcgcgagc ttctcgccgc
     1081 cgtggcggag cagggggcga tggtccgcga cgccggctcc gtcccctaca cgaaccagta
     1141 cgtcgggacg acgtatcttc tctacgagct ctccgagatc gtcctctggg ggctcggccc
     1201 cgcgctcggc ctcgccgccc tcttcggcgt cgcgctccgg ctgcgcgaga cggcacgcga
     1261 gagggagagg cgcgagctcc tcctttggct ctgggccgtc ccgtttctcc tcctgaccgc
     1321 ctcgttcgag gttcgcttcc cgcgctacct cctcccgctc cacccgctcc tcctcctcgg
     1381 cgccgccgcg ctcctcgcgc cgcgcgccgg agaggggagc ggccggcgct tcgcccgacg
     1441 cgccgtcctc ggcctctcgg gcgcgtacct cctcgcgttc ctctccatct acacgcggcc
     1501 ccactccgtc gtctccgcct cgcgctggtt ccacgagaac gcggccgagg gggcgacgct
     1561 cctcgtcccc cactgggaag agggattccc cttcgccctc gacggcctcc cggcgggccg
     1621 cttccgcacg gccgacctcc ccctctacga ccccgacggc gcggagaaga cgcgcctcat
     1681 ggccgagagg ctcgcctccg cggacctcct cgtcttcccg acgaagcgcc acaccggcgc
     1741 cattccgcgc gcgcgagagc gctaccccga caccgaccgc ttcctccgcc tcctctactc
     1801 gggcgacctc ggctggaagc tcgtccgcac gttctcttcg cccccgcagc tcttcggact
     1861 gcgcctgccg accgagctcg ccgacgagtc gttctccgtc tacgaccacc cgcgcgtcac
     1921 cctcttccgg aacgccgccc gcctcgacgc ggcggccctc gagcgcctcc tgcgcgaggc
     1981 gacgccctcc cgcgtcctca cccggcggga gatcctccgc gcccgccccg acgacccgca
     2041 cgccgactcg gaggagagga tccggggcac gcgctccacg cccctcgcgc tccttctctg
     2101 gctcgcgctc ctcgaggcgc tcggcctcgc cgcctggcgc ctcctcgggc cgcgcctccc
     2161 cgccgtgccg ggcgtctacg ccctctcgaa ggtggccggc gtcgccctct tcggcctcgc
     2221 cgcgtgggcc ctcgtcgcct ggggcctctt cgcgttcgtc ccctccttcg ccctcgccgc
     2281 cgccgtcgcc gtcgtcctcg cgggcgccct ctcctcccgc ccgctgcccg cgctcccgcg
     2341 ccgcgagctc ctcgccaccg aagggctctt cctcggcgtg ttcctcttct tcgccgcggt
     2401 ccgcgcgttc tcgccggaga tctactgggg tgagaagccg atggacttct cgttcctcaa
     2461 cgcgctcctt cgctcggagc tccttccgcc gccggagccg tggctcgccg gtacgacgct
     2521 ctcgtacacc tacttcggcc acttcctcgt cgcggcgttc gcgaaggccc tcggcgtcca
     2581 cccggcgctc gccttcaacc tcggcctcgc ggcgaccgcc gcgctcaccg ccgccgcgct
     2641 cctcgccgcc ggggcgttcc tcggcgggcg cctccggacg gggctcctcg ccgccggcct
     2701 cggcctcttc gccggcaacc tcgccggcct cctcgagtac gcgcggcgcc gcgtggtcga
     2761 cttcgacctc ttctggtcgg tctcgcgcgt cctgaagacg cagaacgaga tcaacgagtt
     2821 cccgttctgg agcttcctct tcgccgacct ccacgcccac ctcctcgcct tcccgttcct
     2881 cctcggcctc ctcgccgtcc tcctcctcgc atttcggcag cggacgagcc ccgagctcta
     2941 cgtccccccg ttcggccgct cgctcctcct cgccctcgcg tcgctcttcc tcgccgtcct
     3001 cctcgtcacg aacggctgga gcgcgccgac cgccgccggc ctcctcgccc tcctcctctc
     3061 cgcctccgcc ctcgcggcga cgccgcgccc cgcgccgccg ctcccggcgc ggcgcctcgg
     3121 ccgcctcctc gccggcgtcg tcgtcccggg cctcgccgtc gcgctcccgg cgctcctcgt
     3181 cacgctcccg ttctggcgcg cctacacgcc gccgccgcgg cacctcggct gggagcgggg
     3241 cccgggctac gacgccctct cgctcctcct cgtccacggc ctcggcctcg ccctcgtcgt
     3301 cccgttcctc ctcgcccgct tgcgcgagac gcggcacgac ccggcccggc cgcgccgcgc
     3361 cgccgcgctc gccggaggtc tcgcgctcct cctctctctc tccctcgtcg acctgccggc
     3421 gctcgcgacg ggtcgcctcg ccccggcgcc gtccgtccgc gccttcctcg ccggcctcgc
     3481 cctcctcgcg ctcctcctcg cgttcggccg cggcctcccg cccgcgcggc gcgcgctccc
     3541 cgccgtcgcg gcggcggctt tcggcctcct caccctctgc gaggtcgtct tcgtctggga
     3601 ccggatgaac accgtcttca agttcggcct cgacgccacg ctcctcctcg ccgtcgccgg
     3661 ggccggggcg ctcgacctcc ttctcgagcg ctctccgcgg cccgctccct cccgccggct
     3721 cgccgcgacg ctcgccgccg cggcgctcct cggcgccctc gccacctcgc tcctcgccct
     3781 cggcggacac ctccggacgc gccgcgtcga gacgccgcgc gggaccctcg acggcctcgc
     3841 cttcctccgc agccaccgcc ccggcgaggc cgcggccctc gacttcctcg agatgcgcgt
     3901 gccgggcctc ccgacgatcg tcgaggcctg ggggccgtcc taccgcgaat acgcccgctt
     3961 cgcctcgcgc tccggcctcc cgaccgtcct cggctgggac taccacctcc tccagcgcgg
     4021 ccagccccgc cccgagctcg accggcgcat cctcgacgtc gcccgcctct acgatcccct
     4081 ttcgctctac gacctccccg agatcctctc gcgccgcgag gtccggtggg tcgtttcggg
     4141 ggccgaggag gtcgaggcgc acggggagac gcaccgcgct ctcttccgcg ccttccccgg
     4201 cctcctcgtc cccgccttcg agtcgggcgg cgtcacgctc taccgggtgc cgctccgcgg
     4261 gcgttacgaa acggaacacc tcgtttccgc gaaaccatcc cgcagggcgg accccgcaaa
     4321 gccaaacgac tgattcagaa gactttcgcc ctcacacccc agtcccggcc cgcgagttgc
     4381 tctgttcctg ccgcagggtc ccgccgagcg gggcatgagg aggagagcag atgccaacgg
     4441 gtcgttgggc tccgcccggg aaaagacgga ggggcttctc gctcatcgag ctcctcgtcg
     4501 tcgtcgtcat catcgggatc tcgctgacgc tcggcctctc cggcatcctc tccgcgctca
     4561 agcgtcagcg tctcgcgacg gcggcgagcg aggtgaacaa cctcgtcacg aaggtctact
     4621 cgacgatgca ggacgagaac cttcgaacgt tcctcgtctt cggcaagtac gtcgagggct
     4681 cggggaccga cgtcgccgtc gtcgtcgacc gaaacgacaa cggcgtctgt gacgaggcga
     4741 tcgatccgaa caacgacggc ctcctccacg acgcaccgac gaacctcgtc ccgtggcggg
     4801 ttcgcgtccc cgaggacgtc gccctctcga acgtggcggc gaacggccag gtcttcaccg
     4861 gggcctggct ccgcccgagc ggctccggcc cggtctccgc ggtcctcctc tgcgacttca
     4921 tgggccgggc gatcaccccg ccggaagcgg tgggtggaac gcccgccgtc gccacggggc
     4981 tcgcgacggt ccagctcagt caccgggaca tgatttccgg ctccctgtcg cccctggtca
     5041 cctacaccgt ctcgatcggc ccactcttca agtcgcttaa ctaaagtttc tttgcccaaa
     5101 tctaggag
//
LOCUS       DAOTEZ010000322         5097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_11829_length_5097_cov_6.325069, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000322 DAOTEZ010000000
VERSION     DAOTEZ010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11829_length_5097_cov_6.325069"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="P5164_14850"
     CDS             <1..1089
                     /locus_tag="P5164_14850"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HRY45221.1"
                     /translation="AEASHAVTSARLLQRERDSYRFLDRLREVGRAFATTFDGPRIKH
                     TLCEHAVSLLAGESALFWDADAQTKSLRVLAQGGSDPSSDAPRHVPTENAGHPVVRAW
                     SEKSLVLVDGAEIGALYPGEGSSRGLRAIAAPLLFQDEQIGVLTFAFRSPGGPWPAEL
                     AGRLSLLADTAAVALHNSRLMKIIEQQTERDGATGLYNQGAIVKRLESELKRAERSGL
                     PLSVAHVRIDGLEEAARRFGTPYGDALLPKIAAQLVRATRSVNIVGRGKGDRFWILVF
                     DADKPTAQRAAEAIAKNFSSFRDTRLDATGLRFSLTLGLAAYPEDAFDTASLLSRAEE
                     ALDDAARGGPGSIALWGALAGADVADPL"
     gene            1209..3332
                     /locus_tag="P5164_14855"
     CDS             1209..3332
                     /locus_tag="P5164_14855"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRY45222.1"
                     /translation="MAVTPGTLLGRYRLLERAGAGGMSEVWRAEDQTLHRTVAVKVIH
                     DPIAADPTFAERFLREARLVAGLEHPNILAVYDFGTAPLEGKEVSYLVMPLVSGGSLK
                     ERITGPVPPAQAVPWLAAIAAALDHAHAKGILHRDVKPGNVLLDTSGRPLLADFGLAR
                     SADSVSGLTATGTVMGTPSYMAPEQAMGKALDARADQYALACIAFEMLTGRVPFKADT
                     PLGVLHQHVSVPPEPVTSYVGGLPPGVDDVLSRALAKDAWRRYGSCTELVEALAEVFG
                     LSLGPAAAASAPVRAVPPPPPGAPTIHGAPTVLEAGGARVAADLPTVATAGAGVVPSA
                     GISSPDTAMAAAASMPPPPPAAPARAGKSPALLAAAAVAGLVVLGGGGWLAYSRFGRE
                     AAPGEKPAALASLAPGATSSTESAPAQAVPTGTAADRFGSSGSGASTETGPGTPPEAA
                     STPLAAAAPVEESSPMGQVAETRPGAGEAKPAEGVGKGRRGSDSGGVLSGLDSGPRDS
                     GAVVSADPGLPRREALVDGGQWSLDTRLEGAYRALDTTRSKGTLTREALAEAGGLARE
                     IASTSDEPEARFIDLYTRAGSAFLNGDNATAWQQLHEAFRVDASGAAGGRVLRFVRRV
                     MKGKGMNPGPDANWILGLAFGDVRGDLDEELAAAAERAPGNRAVRVARALRAVEVRDG
                     RRAGALLREACADGIEDACELLGRR"
     gene            complement(3407..4309)
                     /locus_tag="P5164_14860"
     CDS             complement(3407..4309)
                     /locus_tag="P5164_14860"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HRY45223.1"
                     /translation="MKHRLVVAAGLLAVLASSTALAAKPRVAVIEFKDKTQHYSWYRA
                     GEAAQDMLVTELVKSGKYRVIEREQLAAIMQEKNLSLSGEIDPKTAVKAGKLLGVEYI
                     VTGALTELGVADRGARVPGVFGIPSVRVGSQKMDAAIDARMINTTTGEIVWADSAKES
                     TSDAQVFVAGAGGGVDDKRKLDRVLRPVVVKLAESMGKVGVSTSGLGGASDASGIAGK
                     IAKVEGGSVFLNVGAEAGVKEGDTFDVYRVGNVIKDPDTGEVLGSDETKVGRVKITKV
                     MGARLSTASVVSGSGFKPGDMIKN"
     gene            4388..4684
                     /locus_tag="P5164_14865"
     CDS             4388..4684
                     /locus_tag="P5164_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRY45224.1"
                     /translation="MDTEEDVPVILPVEDAIDLHPFRPSETASVVEEYLAAAAGHGFA
                     EVRIVHGRGIGAQRRIVRAVLARSPLVVEFRDAPPERGGWGATVARLRTGGAVR"
     gene            4700..>5097
                     /locus_tag="P5164_14870"
     CDS             4700..>5097
                     /locus_tag="P5164_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HRY45225.1"
                     /translation="MSNAPRTTVIRSSGSYVPPQVVTNEMISRIMDTTDEWIVPRTGI
                     RERRFCAPGVGPAELAEHAARRALEKAGLAPSDIGLVVFATMTPDHYFPGSGGLLAAR
                     LGLTTTPALDIRMQCAGFLSGLQTADAFIRS"
BASE COUNT          645 a         1936 c         1830 g          686 t
ORIGIN      
        1 gcggaggcct cccacgcggt cacgtccgcc cgcctgctgc agcgggagcg cgactcgtac
       61 cgcttcctcg accgcctgcg cgaggtcgga cgcgcgttcg cgacgacctt cgacggcccg
      121 cgcatcaagc acaccctctg cgagcacgcc gtctcgctcc tcgccggaga gagcgcgctc
      181 ttctgggacg ccgacgccca gaccaagtcg ctccgggtcc tcgcccaggg cgggagcgac
      241 ccgagctcgg acgctccgcg ccacgtgccg accgagaacg ccggccaccc ggtggtccgc
      301 gcctggagcg agaaatcgct ggtcctcgtc gacggggcgg agatcggggc gctctacccg
      361 ggcgaaggct cgtcccgcgg gctccgggcc atcgccgcgc cgcttctctt ccaggacgag
      421 cagatcggag tcctcacgtt cgcgtttcgc tcgccgggcg ggccgtggcc ggccgagctc
      481 gcgggccggc tctcgctcct cgccgacacc gccgcggtcg cgctccacaa ctcgcggctc
      541 atgaagatca tcgagcagca gaccgagcgc gacggcgcca ccggcctcta caaccagggg
      601 gcgatcgtca agaggctgga gtcggagctg aagcgcgccg agcggagcgg cctgccgctg
      661 tcggtcgccc acgtgaggat cgacggcctg gaggaggccg cgcggcgctt cgggactccc
      721 tacggggacg cgctccttcc gaagatcgcc gcccagctcg tccgtgccac gcggtcggtg
      781 aacatcgtcg gccggggcaa gggcgaccgc ttctggatcc tggtgttcga cgccgacaag
      841 ccgacggcgc agcgggcggc cgaggcgatc gcgaagaact tctcctcgtt ccgcgacacg
      901 cgcctcgacg cgacgggcct ccgcttctcc ctgacgctcg gcctcgccgc ctacccggag
      961 gacgccttcg acacggcgag cctcctctcg cgcgccgagg aggcgctcga cgacgccgcc
     1021 cgcggcggcc ccggctcgat cgccctctgg ggggctctcg ccggggcgga cgtcgccgac
     1081 ccgctgtagc gggcgcccga agcgcccgga aggagccccg gggggccgca ggatcgcccg
     1141 gttccgtgac gcgcttcgca ttcggggcct tccggcggtc gtatccttcc cccagaaacg
     1201 aggacgccat ggccgtgaca cctggaaccc tcctgggccg ctatcgcctc ctcgagcgcg
     1261 ccggcgccgg cggaatgtcg gaagtgtggc gggccgagga ccagacactg caccgcaccg
     1321 tcgccgtgaa ggtgatccac gacccgatcg cggccgaccc gacgttcgcc gagcggttcc
     1381 ttcgcgaggc gcgcctcgtg gcggggctcg agcatccgaa catcctcgcc gtctacgact
     1441 tcgggacggc gcccctcgag gggaaggagg tctcgtacct cgtcatgccc ctcgtctccg
     1501 ggggctccct caaggagagg atcaccgggc ccgtccctcc cgcgcaggcc gtgccgtggc
     1561 tcgcggcgat cgccgccgcg ctcgaccacg cgcacgccaa ggggatcctc caccgcgacg
     1621 tgaagccggg gaacgtcctc ctcgacacct cgggccggcc gctcctcgcc gacttcggcc
     1681 tcgcccgcag cgccgactcc gtgtcggggc tgacggcgac cgggaccgtc atggggaccc
     1741 cgtcgtacat ggcacccgag caggcgatgg ggaaggccct cgacgcgcgg gccgaccagt
     1801 acgccctcgc ctgcatcgcg ttcgagatgc tgacggggcg cgtgcccttc aaggccgaca
     1861 cgccgctcgg cgtccttcac cagcacgtca gcgtcccgcc ggagcccgtc acgtcgtacg
     1921 tcggcggcct tccgccgggc gtggacgacg tcctgtcgcg cgcgctcgcc aaggacgcct
     1981 ggcggcgcta cgggagctgc acggagctcg tcgaagcgct cgcggaggtc ttcggcctct
     2041 ccctcggccc cgcggccgcc gcttccgcgc ccgtccgcgc agtgcccccg cccccaccgg
     2101 gggcgccgac gatccacggc gccccgacgg tcctcgaggc gggaggggcg cgtgtcgcgg
     2161 ccgacctccc gaccgtcgcg acggcgggcg ccggcgtcgt cccgtccgcc ggaatctcca
     2221 gccccgacac ggcgatggcg gcggcggcct cgatgccgcc cccgccgccg gccgcgccgg
     2281 cacgagccgg gaagagcccc gcgcttctcg ccgccgcggc cgtcgccggc ctcgtggtcc
     2341 tcgggggagg cggctggctc gcctactccc gattcggcag ggaggccgca cccggggaga
     2401 agcccgcggc cctcgcgagc ctcgcccccg gggcgacctc gtccaccgag tccgcgccgg
     2461 cgcaggccgt cccgaccggg acggcggccg accgcttcgg cagctcggga agcggagcgt
     2521 cgacggagac gggccccggc acgccgccgg aggccgcgag cactcctctc gcggcggcgg
     2581 cgccggtcga ggagagcagc ccgatgggcc aggtcgccga gacgcgcccc ggtgccgggg
     2641 aagcgaagcc cgcggagggc gtcggcaagg gccggcgggg ctccgactcg ggcggcgtcc
     2701 tctcggggct ggattcgggg ccgcgggata gcggtgccgt cgtgtccgcc gatccgggcc
     2761 ttccgcgccg cgaagcgctc gtcgacgggg gccagtggtc gctcgacacg cggctcgaag
     2821 gggcctatcg cgccctcgac accacccgct cgaaggggac cctcacgcgc gaggcgctcg
     2881 cggaggccgg cggcctcgcg cgcgagatcg cctccacgag cgacgagccg gaggcccgct
     2941 tcatcgacct ctacacgcgg gccgggagcg cgttcctgaa cggcgacaac gccacggcct
     3001 ggcagcagct gcacgaggca ttccgcgtcg acgcgtcggg cgcggccggg ggccgggtgc
     3061 tccggttcgt ccggcgcgtg atgaagggga aggggatgaa cccgggtccc gacgcgaact
     3121 ggatcctcgg cctcgcgttc ggcgacgtgc gcggcgacct cgacgaggag ctcgccgcgg
     3181 ccgcggagcg ggcccccggg aatcgggcgg tccgggtcgc ccgggcgctt cgcgccgtcg
     3241 aggtccggga cgggcgccgg gccggcgcgc tcctccggga ggcctgcgcg gacggcatcg
     3301 aagatgcctg cgagctcctc ggccggcgct aggcgtcctt cggaggcgaa gacgaagagc
     3361 ggcgggcccg aaggcccgcc gctcttcgtc cggatgcgga gcgacgtcag ttcttgatca
     3421 tgtcgcccgg cttgaagccg gagccgctca cgacggatgc ggtggagaga cgggcgccca
     3481 taactttcgt gatcttcacg cggccgacct tcgtctcgtc ggagccgagg acctcgccgg
     3541 tgtccgggtc cttgatgacg ttgccgaccc ggtagacgtc gaacgtgtcg ccctccttca
     3601 cgccggcctc ggcgccgacg ttcaggaaga ccgagccccc ctcgaccttc gcgatcttcc
     3661 cggcgatccc cgaggcgtcg ctcgcgccgc cgaggccgga ggtcgacacg ccgaccttcc
     3721 ccatcgactc ggccagcttg acgacgacgg gacggaggac gcggtcgagc ttccgcttgt
     3781 cgtcgacgcc gccgccggcg cccgcgacga agacctgggc gtcggaggtc gactccttcg
     3841 cggagtcggc ccagacgatc tcgccggtcg tcgtgttgat catccgggcg tcgatcgcgg
     3901 cgtccatctt ctgcgagccc acccgcacgg aggggattcc gaagacgccg gggacacggg
     3961 ccccgcggtc tgcgactccg agctcggtga gggcgccggt gacgatgtac tcgacgccga
     4021 ggagcttgcc cgccttcacg gcggtcttcg ggtcgatctc gccggagagg gagaggttct
     4081 tctcctgcat gatcgccgcg agctgctcgc gttcgatgac gcggtacttc ccggacttga
     4141 cgagctcggt gacgagcatg tcctgcgcgg cctctccggc gcggtaccag gagtagtgct
     4201 gcgtcttgtc cttgaactcg atgacggcga cgcgcggctt ggcggcgagc gccgtggacg
     4261 aggcgaggac cgcgaggagc cccgcggcca cgacgagtcg gtgcttcatg gctttcctcc
     4321 gttcggcggc cattctccgc cgccggacgc gctccttcaa ccggcgtgcc ggccctacac
     4381 tgtgggcgtg gacacggaag aggacgttcc cgtgatcctc ccggtcgagg acgcgatcga
     4441 cctgcacccc ttccggccgt cggagacggc gtccgtcgtc gaggagtacc tcgcggcggc
     4501 ggccgggcac ggcttcgccg aggtccggat cgtccacggg cggggcatcg gcgcgcagcg
     4561 gcggatcgtc cgcgcggtcc tggcccggtc gcccctcgtc gtggagttcc gcgatgcgcc
     4621 cccggagcgc gggggctggg gtgcgaccgt cgcccgcctt cggaccggcg gggccgtccg
     4681 ctagacttcc gccccgccga tgagcaacgc gccgagaacg accgtcatcc gctcctccgg
     4741 aagctacgtg cctccccagg tcgtcacgaa cgagatgatc tcccggatca tggacacgac
     4801 ggacgagtgg atcgtcccgc ggaccggcat ccgcgagcgg cgattctgcg cgcccggcgt
     4861 cggcccggcc gagctcgccg agcacgccgc ccgtcgggcg ctcgagaagg cgggcctcgc
     4921 gccctccgac atcggcctcg tcgtcttcgc gacgatgacg ccggaccact acttcccggg
     4981 gagcggcggc ctcctcgcgg cgcgcctcgg gctgacgacg acgccggcgc tcgacatccg
     5041 catgcagtgc gccggcttcc tctccgggct ccagacggcc gacgccttca tccgctc
//
LOCUS       DAOTEZ010000323         5070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_11910_length_5070_cov_3.802393, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000323 DAOTEZ010000000
VERSION     DAOTEZ010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11910_length_5070_cov_3.802393"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="P5164_14875"
     CDS             <1..366
                     /locus_tag="P5164_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45226.1"
                     /translation="GMVSDEEITEALASARSLEQIVRGLIDLANERGGVDNITAITVE
                     VRNGEAKKADTSREKTSVSLRAAKVDDEDLTPTDEIPVPVVEAAPETPPSGLPAKDEE
                     SSAPAGGEAEGAPGPEPKG"
     gene            550..768
                     /locus_tag="P5164_14880"
     CDS             550..768
                     /locus_tag="P5164_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45227.1"
                     /translation="MSSIKVNPGDTYQAHVGGNGAAGIVAVAITVHRPTGRVVEEWTA
                     EDFAILGARVSDIMDDVYTVTPYTEDGR"
     gene            765..1193
                     /locus_tag="P5164_14885"
     CDS             765..1193
                     /locus_tag="P5164_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45228.1"
                     /translation="MSTAKTTALAAPPASSLFDPRPGFEVLGGVVASVNVLAVLAAGG
                     WTLGDVLSRHLINRNEATWETDLEIPGAGYVRLLTLSGGGVRPSTYLSTDGEEVLPGH
                     LRVAPAVTLSFYGGASPEDFARAKGRRKRLDRFGQEMKEA"
     gene            2062..2334
                     /locus_tag="P5164_14890"
     CDS             2062..2334
                     /locus_tag="P5164_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45229.1"
                     /translation="MPKPNRSGAAPRTRPRPDLVLALRRTDAATALGWTEGYLRQREA
                     DGTAPPHVRTGKVVIYPLNRLEEWIQRNTIDPSVIGPRPVGTRPAA"
     gene            2533..2796
                     /locus_tag="P5164_14895"
     CDS             2533..2796
                     /locus_tag="P5164_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45230.1"
                     /translation="MTPETEVLLRFDVDEVLASPRRRPLPPPEVLAAARRRNKAGAFL
                     FLQAAGRRGVVSDDPRTEALLAAFRRDALRERGVDERLVRGAA"
     gene            2793..4181
                     /locus_tag="P5164_14900"
     CDS             2793..4181
                     /locus_tag="P5164_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45231.1"
                     /translation="MRGAVPYRETDALLDQEVKAGLEELEEKEAARGELLPLPPVTPW
                     PEPLHPAALRGLAGEIVRAVEPHSEADPVGILVSTLVRFAATVGRSPHCMVGSTRHGV
                     NLFALLVGSTSQGRKGTAEADAEGLFRLAGEESLVPTTSGLGSGEGLVNAIRDEVKKS
                     KVVKEKGGSTHVEDYVAEDGVEDKRLLVREAEFAHPLRVAQREGSVLSPVIRLAFDGA
                     DLRITTRNTPLRASNPHVCILGHIPPEEERRLLADAEVESGFANRFAHFAVRRSKLLP
                     NVRPVPRDVAVPLAARLREAVSFGRAVGEMGRDAAAEDLWVRLYVVLAEERPGLAGAL
                     LARAHAHVLRLSLVYALLDQSIVVRRAHLESAAALWAYSESSVRYVFGDMLRDRRAEE
                     LVTALRDAGAKGLKREEIRDLFSRNLSAVRVDMLLGDLARQGLAHARKAAGRGPGRPA
                     VTWYPGPAPEVS"
     gene            4585..4890
                     /locus_tag="P5164_14905"
     CDS             4585..4890
                     /locus_tag="P5164_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45232.1"
                     /translation="MTAAARLERFALAMELLEGLEARLAAAIEEEAVVPGQIPEDVGD
                     ALRRQSENVLSLALRWDDLLEAAQALGLPEPFGGREAYAAYLQSLRLYGAETAETVQ"
BASE COUNT          805 a         1820 c         1756 g          689 t
ORIGIN      
        1 ggaatggtct cggacgagga gatcaccgag gccctcgctt cggctcgctc gctggagcag
       61 atcgtccgcg ggctgatcga cctcgccaac gagcgcggcg gcgtcgacaa catcaccgcc
      121 atcacggtcg aggtgcggaa cggagaggcg aagaaggccg acacgtcccg ggagaagacc
      181 tccgtctcgc tccgggccgc gaaagtcgac gacgaagacc tgacgcccac cgacgagatt
      241 ccggtccccg tcgtcgaggc cgcgcccgag acgcctccct ccggcctccc cgcgaaggac
      301 gaggagtcct cggcccccgc cggcggcgag gccgaagggg cgcccggccc cgagccgaag
      361 ggctgaagcg cctcccggtc cggcggccgc gccggagccc cgtcagacgt tgagcacaga
      421 ctcgctccgt gagtagtgaa aggcgcgtat ctagcggact tcgcaacgga gcgactcgga
      481 cgcgacccgc atagaacgca cctttccggc accgtggcgc cactgacggc gccatcggaa
      541 aggagcgaga tgagcagcat caaggtcaac ccgggcgaca cgtaccaggc tcacgtcggc
      601 ggcaacgggg ccgcgggcat cgtcgccgta gcgatcaccg ttcaccggcc gacggggcgc
      661 gtcgtcgagg agtggacggc cgaggacttc gcgatcctcg gagcccgcgt ctcggacatc
      721 atggacgacg tctacaccgt caccccgtac acggaggacg gacgatgagc accgcgaaga
      781 cgacggcgct tgccgcgccc ccggcatcgt ccctcttcga tccccgcccc ggcttcgagg
      841 tcctcggggg agtcgtcgcg agcgtcaacg tcctcgccgt cctggccgct ggcggatgga
      901 ccctcggcga cgtcctctcc cggcacctca tcaaccggaa tgaggcaacg tgggagaccg
      961 acctcgaaat ccctggggcc ggctacgtcc gcctgctcac cctgagcggc ggcggggtca
     1021 ggccctcaac gtacctcagc acggacggtg aggaggtcct tcccgggcac ctgcgcgtcg
     1081 cgccggccgt cacgctctcg ttctacggcg gcgcgtcccc ggaggacttc gctcgggcga
     1141 agggccggcg gaagcggctc gatcggttcg ggcaggagat gaaggaagcc tgatcattcc
     1201 tccgggccgg cgggcggcgc ggctgatcac cgcgccgaag cgacttcgct cccgcgtccc
     1261 gccggcccgg acctttcctc gaggagcgag cggagcgaca cgtggcgaag acggttcacc
     1321 cgggaacgca ccttgtggag gcggcgtggg cggcaagagg cgaggcggaa aagagggcgc
     1381 gcgcactcat cgcagaacgc gacgtcgacg agccgctcgg gctcctgctc tctctctccc
     1441 tgccgctcta ctacttgagc gacgccctcc agagcgtcga ccccagccgc agggcggagg
     1501 agcttctttc cgaggccctc gcggaggcca tcgctcgccg ggacgcggag cgagttcgcg
     1561 ccccctcgga gggcacctgg gagacctacg ccgcgctcat cgagcactcc gcatttctcg
     1621 ccgcggccaa ggcggcaccg ggcgaccgcg tgagggccgg cgcggaccgg ctgaagagga
     1681 agctcacatc gaaccctcgc gcagcacatc tcttcggcct ctcctgggcc gtctctgccg
     1741 ctctgcgcga agggcgagcg gacctccgaa atgaggcgtc ccgcgagatg gacgaggtcc
     1801 tcacggagcg ctgggaacgg atcgtcgccg cgtgcttcac gggtcgcgga ccccttgacg
     1861 gggaggccgc gtggaggaag tggcaccaca tccgaggcac cttcaagccg ctgcgcaacg
     1921 cgtggaacag gagatcgaag aaggtctcgc gcttcgtcga caccgtgaag gcaacagtta
     1981 gagaccccca atccgttgcc gtggagcctt cgcgaaaggc ccgtgcaaac cgatccttgc
     2041 aacgaaagga gggcacgacc gatgcccaag cccaacagaa gcggagcggc cccgcgaacg
     2101 cggccgcggc ccgacctcgt cctcgccctt cggcggaccg acgccgcgac ggcgctcggc
     2161 tggaccgagg gctacctccg gcagcgcgag gccgacggga cggcgccgcc gcacgtcaga
     2221 acggggaagg tcgtcattta cccgctcaac cgcctcgagg agtggatcca gcgaaacacg
     2281 atcgacccga gcgtcatcgg cccgcggccc gtcggaaccc ggccggcggc ctgaggaaga
     2341 gaaaggaacc accgtgatct caaatgaaga ggccggctcg ggtctgaacc acccgaaccg
     2401 gccgacgcga tcaacgaagc cttgcgaagc ccattttagc gcgtcgcccg atcctaactc
     2461 cagccgcccc gttctcgtcg accgggatct cgcggtcggc cggctcgccc gctgggcggc
     2521 ccgggggccg gcatgacgcc cgagacggag gtcctcctcc ggttcgacgt ggacgaggtc
     2581 ctcgcgtcgc cgcggcgccg gcccctcccg cctccggagg tcctcgcggc ggcgcggcgc
     2641 cggaacaagg ccggcgcgtt cctcttcctc caggccgcag gccggcgcgg cgtcgtgtcc
     2701 gacgacccgc ggaccgaagc tctcctcgcc gccttccgcc gggacgcgct gcgcgagcgc
     2761 ggcgtcgacg agcgcctcgt ccggggtgcg gcgtgagggg cgcggtcccg taccgggaga
     2821 ccgacgccct cctcgaccag gaggtgaagg ccgggctcga ggagctcgag gagaaggagg
     2881 cggcgcgggg tgaactcctt cccctaccac ccgtcacccc gtggccggag cctctccacc
     2941 cggccgcgct ccgaggtctc gccggagaga tcgtgcgcgc cgtcgagcct cactcggagg
     3001 ccgaccccgt cgggatactc gtctcaacgc tcgtccggtt cgccgcgacg gtgggacggt
     3061 ccccccactg catggtcggg tcgacgcgcc acggcgtgaa cctcttcgcg ctcctcgtcg
     3121 ggtcgacgtc gcagggccga aagggcaccg ccgaggccga tgctgagggc ctcttccgcc
     3181 tcgcagggga ggagtccctc gtcccgacga cgtccggcct cggctccggg gagggcctcg
     3241 tcaacgccat ccgggacgag gtgaagaagt cgaaggtcgt caaggagaag ggtggctcaa
     3301 cccacgtcga ggactacgtc gcagaggacg gagtggaaga caagaggctc ctcgtccgag
     3361 aggccgagtt cgctcatccg ctccgcgtcg cgcagagaga gggctccgtg ctctcaccgg
     3421 tgatccgact cgccttcgac ggggccgacc tgaggatcac gacgcggaac acgccgctcc
     3481 gggcgagcaa ccctcacgtc tgcatcctcg gccacatccc gccggaggag gaacgacgtc
     3541 tactcgccga tgcggaggtg gagagcgggt tcgcgaaccg cttcgcgcac ttcgccgtcc
     3601 ggcggtcgaa actcctcccg aacgttcggc cggtgccgcg cgacgttgcc gtgccgctcg
     3661 ctgcgcgact gcgggaggcg gtctccttcg gccgcgccgt cggggagatg gggcgcgacg
     3721 ccgcggcgga ggacctctgg gtccgcctct acgtcgtcct ggcggaggag cggcctgggc
     3781 tcgccggtgc gctcctggcc cgcgctcacg ctcatgtcct gcgcctctcc ttggtctacg
     3841 cgctcctgga ccagagcatc gtcgtccggc gagcgcacct cgagagcgcg gccgccctct
     3901 gggcctactc ggaatcctcc gtgaggtacg tgttcggcga catgcttcgc gaccgccggg
     3961 ccgaggagct cgtgaccgcg ctgcgggacg ccggagcgaa gggcctcaag cgagaggaga
     4021 tccgcgacct cttctcgagg aacctctcgg ccgtccgggt cgacatgctc ctcggtgacc
     4081 tcgcccgaca ggggttggca cacgcccgga aggccgccgg cagggggccg gggaggccgg
     4141 ccgtcacctg gtaccccggg ccagcgccgg aggtgtcgtg atgccgatgc cgcactccaa
     4201 gatcctcgac ctcctcgagg ccgatccggt cgacgcggag aacgcacggg tctacgcgga
     4261 aaacgcggaa aacgcggtat ttcggcccgg cgagccaatt accgcgttta aatcgttttc
     4321 cgcgtctatc cgtgcgcgcg agagcgaacc cgcggggccg gttcgggccg gggtcgtcct
     4381 cacctgctac gcctgccgcg ggaccgactt ctgggtgggg accgggaagg tgacgtgtcg
     4441 ccgctgccac ccgcccgccc cgggggccga ggtcctcacg ctctcgagga cgggctccga
     4501 tgcgtcgcgg cctgtggaaa tcgaagcagg atcgtcggcg cttacaccgg acacccggac
     4561 acatggagtc cgcggaggtg ccgcgtgacg gcggccgcgc gcctcgaacg cttcgccctg
     4621 gcgatggagc ttctcgaggg tctcgaggcc cgtctcgccg cggcgataga ggaggaggcc
     4681 gtcgtcccgg ggcagatccc ggaggacgtc ggcgacgctc tccgccggca gtctgagaac
     4741 gtcctctccc tcgcgctccg ctgggacgac ctcctcgagg cagcgcaagc cctcggcctt
     4801 cccgagccct tcggcgggcg cgaggcttac gccgcctatc tccagagtct ccgcctgtac
     4861 ggcgccgaga ctgcggagac cgtccagtga accccccgtg ctcctcctcc cgtccggcgc
     4921 accggacgat ccgtcggctg gccagcctgc gaaagcctcc ggacgggagg gggactttca
     4981 cccgaaccga gaaagacgcc gcaacgcggc agaggtagaa cgatgttgaa gctctcgacc
     5041 gacaagcaag tggagctcct ttccgcggcg
//
LOCUS       DAOTEZ010000324         5066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_11923_length_5066_cov_6.276591, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000324 DAOTEZ010000000
VERSION     DAOTEZ010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11923_length_5066_cov_6.276591"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="P5164_14910"
     CDS             complement(<1..748)
                     /locus_tag="P5164_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HRY45233.1"
                     /translation="MRVLILGVNGFIGNALTRRILTTTDWQVFGLDVGSDRIEEFLGE
                     KRFRFLEGDIAINREWIEYHVKKCDVVLPLVAIATPATYVKNPLAVFELDFEENLRIV
                     KMAARYGKRVVFPSTSEVYGMCPDAAFDEDASPLVYGPIRKERWIYSASKQLLDRVIW
                     AMGNANGLRFTLFRPFNWYGPHLDDVDAPKEGSSRVLTQFLHNILYGVPIKLVDGGRQ
                     RRCFTYIEDGIDGLMKILVNENGCADGQIFN"
     gene            complement(768..1682)
                     /locus_tag="P5164_14915"
     CDS             complement(768..1682)
                     /locus_tag="P5164_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HRY45234.1"
                     /translation="MGGIVVCGYSDVGYRCTKWLLDAGEPVRFVYTHPDAPGEERWFE
                     SVAELARARNVPVKLVDAIDAAADEAALRAAAPDFLFSFYFRSMIPARVLSIPSKGAL
                     NMHGSLLPAFRGRAPVNWAILKGAPFTGASLHYMTEKPDAGDLVDQEKVPIGPDDDAL
                     AVSRRVSEAAVAVLARSWPKLKAGTALRIPLDLTKGSYFGARRPEDGLIDWLRPAQEV
                     HDLVRAVSRPWPGAFTDSFGPRVTIWRTRLSGYGGHDTFPGKIDMTERSVLVYCGDDR
                     PVEILAARPEGQPDMNEAEFRSWVLARG"
     gene            complement(1760..2719)
                     /locus_tag="P5164_14920"
     CDS             complement(1760..2719)
                     /locus_tag="P5164_14920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY45235.1"
                     /translation="MTSPPTVPRKVSVVVPVYNEEANLPRLLPRLVPVLEGLGRPWEV
                     VFVDDGSRDGSLELLKAAVAERPGRVKAVELLRNAGQHMAIMAAFGEVDGEYVITLDA
                     DLQNPPEEIPKLVGKMDEGFDVVGTIRSKRKDSLFRKAASRIVNRTTGLLTGMRLNDY
                     GCMLRGYHRDVVDVMTASDEVSTFIPALAQLYARRSVEIDVAHEERAEGESKYSLLRL
                     VRLNFDLMTGFSLVPLQLFGPLGFVTALFGVGFGAFLLVRRLVIGSEVEGVFTLFALA
                     FMVMGVIMAGVGMVGEYVGRIYQQVRGRPRFLVRRVHGAKEEP"
     gene            complement(2796..4535)
                     /locus_tag="P5164_14925"
     CDS             complement(2796..4535)
                     /locus_tag="P5164_14925"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY45236.1"
                     /translation="MGAYLTSGSRFRSEPLAPASRALLWALLLLALAVLVAETGHTPF
                     FEPDEARYAEIPREMLATGDLVAPRLNGFRYYEKPPLHYWAVAASMSLFGESEASARL
                     PVKLSAAGMVLVTVLFARRRYGERVALVAGLVVATSLLVVALGRVNIIDGPLSLALSS
                     AAFAFAAFQEHEQSGDRARARVALYLLHFSCAAAVMLKGFVGLVLPGGAILVWALLSR
                     RLGIVPKLFSPGPLLLFLVLVVPWHVAMARRDGEWFDFYVVGEHFRRYFETGHRRTAK
                     PLFFVYVLLGGLVPWTPYLGRLAGALPSLRRGEWRERGAEAYLQVFWVLVLVFFSASR
                     SKLVPYLLPIWPAVAVLLALGLERARQRGATLRAERRAAGLLFGLLAGAAAAYAFGAG
                     LAVRYGVEVQAAVAVGSLALGALLNLLAGRTAARATRRALSTERFVLGMVLPWLGFSG
                     ALVLALPPVARSITPWPLVATLKRELRPDDLLLQRGHYLQGPAFYTKRLTPVADLPWS
                     ELDFGKEEARQRGLYLSDEEFAKKWHGPARVLCVVHFGHLRDFADPAAGLSIPHVLAR
                     SPNGKFLLVSNRP"
     gene            complement(4520..>5066)
                     /locus_tag="P5164_14930"
     CDS             complement(4520..>5066)
                     /locus_tag="P5164_14930"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HRY45237.1"
                     /translation="EALRAESAAPAPAPAGAEATAGPEAEPAPAERARRWGDRSAAID
                     GVRLLDDDGLERYAFRTGEPVTIEIEATPARPLDDFVFGIGLFTPEDVCVHGTNTEID
                     GLAAGRFDAAARVRVTLARCDLGPGTYLVDVAVHARNGTPYDYWRGACRFRVDSPRTE
                     AGVWAPERSWTVEGGATWART"
BASE COUNT          830 a         1744 c         1841 g          651 t
ORIGIN      
        1 ggttgaagat ctggccgtcg gcgcagccgt tctcgttgac gaggatcttc atgaggccgt
       61 cgatcccgtc ctcgatgtag gtgaagcagc gccgctggcg ccccccgtcg acgagcttga
      121 tcgggacacc gtagaggatg ttgtggagga actgggtcag gacgcggctc gagccttcct
      181 tcggggcgtc gacgtcgtcc aggtgcggcc cgtaccagtt gaacgggcgg aagagcgtga
      241 agcgcaggcc gttcgcgttc cccatcgccc agatgacccg gtcgaggagc tgcttggagg
      301 cggagtagat ccagcgctcc ttgcggatcg ggccgtagac gaggggcgag gcgtcctcgt
      361 cgaaggcggc gtccgggcac atcccgtaga cctccgaggt cgacgggaag acgacccgct
      421 tgccgtaccg ggccgccatc ttgacgatcc ggaggttctc ctcgaagtcg agctcgaaga
      481 ccgcgagggg gttcttcacg tacgtcgccg gagtcgcgat cgcgacgagc gggaggacga
      541 cgtcgcactt cttgacgtga tactcgatcc actcgcggtt gatcgcgatg tccccctcga
      601 ggaagcggaa gcgcttctcg ccgaggaact cctcgatgcg gtcgctcccg acgtcgaggc
      661 cgaacacctg ccagtccgtc gtggtgagga tccgccgcgt cagggcgttt ccgatgaatc
      721 cgttgactcc gaggatgagg acgcgcatcg cgcctttccg gccgcgttca gccccgggcg
      781 agtacccagg aacggaactc ggcctcgttc atgtcgggct gcccctcggg gcgcgccgcg
      841 aggatctcca cgggacggtc gtcgccgcaa tagacgagga cggaacgctc cgtcatgtcg
      901 atcttgcccg ggaaggtgtc gtgcccgccg tagccggaga ggcgggtcct ccagatcgtc
      961 acgcgcgggc cgaaggagtc cgtgaacgcc ccgggccagg gccgcgaaac ggcgcggacg
     1021 aggtcgtgga cctcctgcgc cggccggagc cagtcgatca gcccgtcctc ggggcgcctc
     1081 gcgccgaagt aggagccctt cgtcaggtcg agcgggatcc ggagcgccgt tcccgccttc
     1141 agcttcggcc agctccgggc caggaccgcc acggccgcct ccgagacgcg acgcgagacc
     1201 gcgagggcgt cgtcgtccgg cccgatcggc accttctcct ggtcgacgag gtcgcccgcg
     1261 tcgggcttct cggtcatgta gtggagagag gcccccgtga acggggcccc cttcaggatg
     1321 gcccagttca ccggagcccg cccccggaac gccgggagga gggagccgtg catgttgagc
     1381 gcccccttcg acgggatcga gaggacgcgg gcagggatca tcgaccggaa gtagaacgag
     1441 aagaggaagt ccggggcggc ggcgcgcagg gccgcctcgt ccgcggcggc gtcgatcgcg
     1501 tcgacgagct tcaccggcac gttccgggcg cgggcgagct cggcgaccga ctcgaaccag
     1561 cgctcctcgc cgggagcgtc gggatgggtg tagacgaacc ggaccggctc cccggcgtcg
     1621 aggagccact tcgtgcatcg atacccgacg tcgctatacc cacagacgac gattccgccc
     1681 atgacttacc cccttctcca attcgggggg tccggggggt gcaaggcgaa cgcacccccc
     1741 gggtggccga cgattccgct caaggctcct ccttcgcccc gtgcacgcgc cggacgagga
     1801 agcggggccg gccgcgcacc tgctggtaga tccggccgac gtactcgccg accatcccga
     1861 cgcccgccat gatcaccccc atcaccatga aggcgagagc gaaaagagtg aagaccccct
     1921 cgacctccga cccgatcacg agccggcgaa cgaggaggaa ggcgccgaac ccgaccccga
     1981 agagggccgt cacgaacccg agggggccga agagctggag cgggacgagg gagaagcccg
     2041 tcatcaggtc gaagttcagc ctcacgaggc gaagcagaga gtacttcgac tccccttccg
     2101 ctcgctcctc gtgggcgacg tcgatctcca cggatcggcg ggcgtagagc tgggcgagcg
     2161 ccgggatgaa cgtcgagacc tcgtccgacg ccgtcatcac gtcgacgacg tcccggtggt
     2221 acccgcggag catgcagccg tagtcgttca gccgcatccc ggtcaggagg ccggtcgtcc
     2281 ggttcacgat ccgggaggcg gccttccgga agaggctgtc cttccgcttc gagcggatcg
     2341 tcccgacgac gtcgaaaccc tcgtccatct tcccgacgag cttcgggatc tcctcggggg
     2401 gattctgaag gtccgcgtcg agggtgatca cgtactcccc atccacctcg ccgaacgcgg
     2461 ccatgatcgc catgtgctga ccggcgttcc gaaggagctc gacggccttc acccggccgg
     2521 gccgttcggc aacggcggcc ttcaggagct cgagcgagcc gtcgcgtgag ccgtcgtcga
     2581 cgaagacgac ctcccacggc cgtccgagcc cctcgaggac ggggaccagg cgggggagga
     2641 gccgagggag gttcgcctcc tcgttgtaga cggggacgac gacggagacc tttcgcggga
     2701 cggtcggcgg gctcgtcaca ggacgacgat tctaggggac gtgcggggag gggttcgaac
     2761 gggtcccgaa ccggccgaga gcgccctcca cggcctcacg gacggttcga gacgaggagg
     2821 aacttcccgt tcgggcttcg agccaggacg tgcggtatcg agaggccggc cgccgggtcc
     2881 gcgaagtcgc gcaggtggcc gaagtggacc acgcacagga cccgcgccgg accgtgccac
     2941 ttcttcgcga actcctcgtc cgagaggtag aggccgcgct gccgtgcctc ctccttcccg
     3001 aaatcgagct cgctccaggg gaggtccgcg accggcgtga gccgcttcgt gtagaaggcc
     3061 ggcccctgca ggtagtgccc ccgctggagc aggaggtcgt ccggccggag ctcccgcttc
     3121 agagtcgcca ccagcggcca gggcgtgatc gaacgggcga ccgggggaag cgccaggacg
     3181 agcgcgccgg agaaaccgag ccacggcagg accattccga ggacgaaccg ctccgtggag
     3241 agcgcgcggc gggtcgcacg ggcggccgtc cggcccgcga ggaggttcag gagggcgccg
     3301 agagccagcg agccgaccgc gacggcggcc tggacctcca cgccgtaccg gaccgcgagc
     3361 cccgctccga aggcgtaggc ggccgcggct cccgccagca ggccgaacag gagccccgcg
     3421 gcccggcgct cggcgcgcag cgtcgcgccg cgctgccgcg cccgttcgag gccgagggcg
     3481 aggaggaccg cgacggcggg ccagatcggg aggaggtacg gcacgagctt ggagcgcgag
     3541 gccgagaaga agacgaggac gaggacccag aagacctgca ggtacgcctc ggcgccccgt
     3601 tccctccact cgccgcggcg aagcgagggg agcgcgcccg cgaggcgccc caggtagggc
     3661 gtccacggca cgagccctcc cagcagaacg taaacgaaga agagcggctt cgcggtcctc
     3721 cggtgccccg tctcgaagta cctccggaag tgctccccca cgacgtagaa gtcgaaccac
     3781 tcgccgtccc ttcgggccat cgccacgtgc cacgggacga cgaggaccag gaacaggagc
     3841 agcggccccg gggagaagag cttcgggacg atccccaggc gtctcgagag gagggcccag
     3901 acgaggatcg cgccgcccgg gaggaccagg ccgacgaagc ccttgagcat gacggccgcc
     3961 gcgcaggaga agtgaaggag gtagagcgcg actcgcgcgc gcgcccggtc ccccgactgc
     4021 tcgtgctcct ggaacgcggc gaaggcgaac gccgccgagg agagggcgag cgagagcggg
     4081 ccgtcgatga tgttcacgcg cccgagggcc acgacgagga gggaggtcgc cacgacgagc
     4141 cccgcgacga gggccacgcg ctcgccgtat cggcgcctcg cgaagaggac cgtcacgagg
     4201 accatcccgg cggccgagag cttcaccgga aggcgggcgg acgcctccga ctctccgaag
     4261 agagacatcg aggccgcgac ggcccagtaa tggaggggcg gcttctcgta atagcggaat
     4321 ccgttcagcc tcggcgcgac gaggtcgccc gtggcgagca tctcgcgggg gatctccgcg
     4381 tagcgcgcct cgtccggctc gaagaagggc gtgtgccccg tctccgcgac gaggacggcg
     4441 agggcgagga ggaggagcgc ccagagcagg gcccgggacg ccggcgcgag cggctcggac
     4501 cggaagcggc tcccgctggt caggtacgcg cccacgtcgc ccctccttcg acggtccagc
     4561 tccgctccgg ggcccagacg cccgcctccg tccgcggcga gtcgacccgg aaacggcagg
     4621 ccccccgcca gtagtcgtag ggggtgccgt tccgcgcgtg gaccgcgacg tcgacgaggt
     4681 aggtccccgg acccaggtcg cagcgcgcca gcgtgaccct caccctcgcg gcggcgtcga
     4741 atcggccggc cgcgaggccg tcgatctccg tgttcgtgcc gtggacgcag acgtcctccg
     4801 gcgtgaagag cccgatgccg aagacgaagt cgtcgagcgg gcgggcgggc gtcgcctcga
     4861 tctcgatcgt gaccggctcg ccggttcgga aggcgtagcg ttcgaggccg tcgtcgtcga
     4921 ggagcctcac gccgtcgatc gcggcagagc ggtcgcccca gcgccgcgcc ctctcggcgg
     4981 gggccggctc ggcctcgggt ccggccgtcg cctccgcgcc ggccggcgca ggggcggggg
     5041 ccgccgactc ggcgcggagc gcctcc
//
LOCUS       DAOTEZ010000325         5057 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_11957_length_5057_cov_5.186925, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000325 DAOTEZ010000000
VERSION     DAOTEZ010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11957_length_5057_cov_5.186925"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="P5164_14935"
     CDS             complement(<1..722)
                     /locus_tag="P5164_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45238.1"
                     /translation="MLGLADRSRFRGGLLPRRTFLALLAFASLLGPLLVFRETVRDPL
                     ERIPCGRPVPNEQLGDPVIILSVMGRQWARWDAGDFSRRDDRVFAPYADTSTLGEPWL
                     LPAIVGYPWARLFDSPALGYDAPFLFASVVACFGAGLLLADLAGAGWPALLGALLFAW
                     SPARLNNFGVVGTMWGGFVLLILLFSLRYLRAGRPRDVALAGVALLVTGFGSTYPLVM
                     GGVAVAVVAIVSARSIRRLAVLA"
     gene            797..2494
                     /gene="ggt"
                     /locus_tag="P5164_14940"
     CDS             797..2494
                     /gene="ggt"
                     /locus_tag="P5164_14940"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRY45239.1"
                     /translation="MRASFAALLALAVALPLLAEWPPKGRGDASRSPVWAEHGMVASA
                     QPLCTQAGVDTLRKGGSAVDAAIATNACLGLMEPTANGLGGDLFAILWDPAKKAVVGL
                     NASGRAPLALTADKVPPLPDGTIPLYSPYAWSVPGCGDGWLELHAKYGKLPLAEVLAP
                     AIRYAEEGFPVSPVIAGNWAGSVNRFRDKPGFAEVFLPGGLAPKEGERFRNPALAKTL
                     RLLASKKRAGWEGEFADAIVAFSKANGGLFAKEDFVRHRSTWDAPMSTSYRGYDVWEL
                     PPNTQGLAALQMLNLLEGFDLAKMGRESADFWHVMVEAKKVAYADRARYYADPAFAKL
                     PLERLLSKEYAKERAKKIDVKRASREDPPDEELVLNRKETTYLCAADASGLMVSLIQS
                     NYTGFGSGYAVPALGIGIHNRGGLFSLKKGHPNFLEPGKRPFQTIIPALLTKDGQPLM
                     AFGLMGGDMQPQGHVQVVVNLVDFGMNLQEAGDAIRFHHTGSSEPTGTVMKDGGTLFL
                     EEGLPEPLLEELMRRGHRIAREPVGAYGGYQAVARDPATGFLLGATEKRKDGAAAGF"
     gene            2559..3785
                     /locus_tag="P5164_14945"
     CDS             2559..3785
                     /locus_tag="P5164_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008612245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRY45240.1"
                     /translation="MSVSAEKAVPRVSERGRRVPASPIRKLASYADAAKKRGVKVYHL
                     NIGQPDLETPAPMRDRLAQIKDKVYAYTPSNGTAECLDFLVGYYRGRGVELTKSQVIT
                     TTGGSEAIQFAFMACCEVGDEALLVEPFYTNYRAFAAMGGIDLKPIVTRAEDGFHLPS
                     REEWEKALTPKTKAVLLCNPNNPTGTVYSKEELVRVAEFCRDHGLFLIADEVYREFVY
                     DGRQVESALSLEGFEDVVVVVDSLSKRYSACGIRLGCLATRNKDVYQACLHMAQGRLS
                     PPGLAQAIAPGAALLGPDYVEGVVKEYGHRRDILYEELTKIPGIFFRKPEGAFYFVAR
                     IPVKDSEDFAQWLLTDFQLDGATVMVAPADGFYATPGLGKDEVRIAYVLKAEDLKAAC
                     RVLEAAIPAYRAARGL"
     gene            4282..4638
                     /locus_tag="P5164_14950"
     CDS             4282..4638
                     /locus_tag="P5164_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45241.1"
                     /translation="MTAAAVEGFPPLPSYTAKVRNVSNRPIRRVVATVVYLDAAGRPM
                     PGEEHDVSFGSPLKAIDPGLTFESVFLSRVDRAPAVRLVPRAVTVLEKGDGPEPIERE
                     WKNPHYDEELHRAFRP"
     gene            complement(4702..>5057)
                     /locus_tag="P5164_14955"
     CDS             complement(4702..>5057)
                     /locus_tag="P5164_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRY45242.1"
                     /translation="LTSILFLLARGEVSRWHRVLSDEVWHFHEGDPLELLVVDAGFAG
                     ATRHVLGRASQGNEPLAVVPAGAWQAARPLGRYALVACDVGPGFDFADFVLLAELPDE
                     SARLRERLPTLVELL"
BASE COUNT          801 a         1890 c         1691 g          675 t
ORIGIN      
        1 gcgagaacgg cgagccgccg gatgctgcgc gccgagacga tggcgacgac ggcgaccgcg
       61 acgcctccca tgacgagcgg gtaggtgctt ccgaaaccgg taacgaggag cgcgactccc
      121 gcgagggcca cgtcgcgcgg ccggcccgcc cggaggtacc ggagcgagaa gaggaggatg
      181 agcaggacga accctcccca catcgtcccg acgacgccga agttgttgag tcgcgccggg
      241 ctccaggcga agagcagggc gccgagaagc gccggccagc ccgcgccggc gaggtccgcg
      301 aggagcagtc ccgccccgaa gcaggcgacc acggacgcga agaggaacgg cgcgtcgtac
      361 ccgagcgccg gagagtcgaa gagtcgagcc cacggatagc cgacgatcgc cggaagcagc
      421 cagggctcgc cgagagtcga cgtgtcggcg tacggcgcga agacgcggtc gtcgcgccgc
      481 gagaagtctc ccgcgtccca ccgcgcccac tgccggccca tcaccgacag gatgatgacg
      541 gggtccccca gctgctcgtt cgggaccggc cggccgcagg ggatcctctc gagcgggtcg
      601 cgaaccgtct cgcggaagac gaggagcggt ccgaggagcg acgcgaacgc gaggagcgcg
      661 aggaatgtcc ttcgcgggag gagccccccc cggaacctcg accgatcggc cagcccgagc
      721 atcggcgtca gtctagaacg ccctcgaagc cttctgttcc tccgctttcg gcgccggctc
      781 cgcgtacgat cccctcatga gagcgtcctt cgccgccctc ctcgccctcg ccgtcgccct
      841 ccccctcctc gccgagtggc cgccgaaggg gcgcggcgac gcgagccgct cccccgtctg
      901 ggccgagcac ggcatggtcg cgagcgccca gccgctctgc acgcaggcgg gcgtcgatac
      961 gctgcgcaag ggagggagcg ccgtcgacgc ggcgatcgcc acgaacgcct gcctcgggct
     1021 gatggagccg acggcgaacg gcctcggcgg cgacctcttc gcgatcctct gggaccccgc
     1081 gaagaaggcg gtcgtcggcc tgaacgcctc ggggcgggca ccgctcgccc tgacggccga
     1141 caaggtcccg ccgcttcccg acgggacgat cccgctctac agcccgtatg cctggagcgt
     1201 cccgggctgc ggcgacgggt ggctcgagct ccacgcgaag tacggaaagc ttccgctcgc
     1261 ggaagtcctc gcgccggcga tccgctacgc cgaggagggc ttccccgtct cgcccgtcat
     1321 cgcggggaac tgggcggggt cggtgaaccg cttcagggac aagcccggct tcgcggaggt
     1381 cttcctcccg ggcggccttg ccccgaagga gggcgagcgc ttccggaacc cggccctcgc
     1441 gaagacgctc cggctcctcg cgtcgaagaa gcgggccggc tgggaaggcg agttcgccga
     1501 cgccatcgtc gccttctcga aggcgaacgg cggccttttc gcaaaggagg acttcgtccg
     1561 tcaccgctcg acctgggatg ccccgatgtc gacgagctac cggggctacg acgtctggga
     1621 gctcccgccg aacacgcagg ggctcgcggc gctccagatg ctgaacctcc tcgaggggtt
     1681 cgacctcgcg aagatgggga gggagagcgc cgacttctgg cacgtcatgg tcgaggcgaa
     1741 gaaggtcgcc tacgccgaca gggcccgcta ctacgccgac ccggccttcg cgaagctccc
     1801 gctcgagcgg ctcctctcga aggagtacgc gaaggagagg gcgaagaaga tcgacgtgaa
     1861 gcgcgcctcc cgggaggacc ctcccgacga ggagctcgtc ctgaaccgga aggagacgac
     1921 gtacctctgc gcggccgacg cctcgggcct catggtctcc ctcatccaga gcaactacac
     1981 cggcttcggc tcgggctacg ccgtcccggc gctcggaatc gggatccaca accggggcgg
     2041 cctcttctcc ctgaagaagg ggcacccgaa cttcctggag cccgggaagc gccccttcca
     2101 gacgatcatc ccggccttgt tgacgaagga cggtcagccg ctcatggcct tcggcctcat
     2161 ggggggcgac atgcaaccgc aggggcacgt gcaggtcgtc gtgaacctcg tcgacttcgg
     2221 gatgaacctc caggaggcgg gcgacgcgat ccgcttccat cacaccggct cgagcgagcc
     2281 gaccgggacg gtcatgaagg acggcggcac gctcttcctc gaggaagggc tccccgagcc
     2341 gctcctcgag gagctgatga gacgcggtca ccggatcgca cgggagcccg tcggcgccta
     2401 cggcggctac caggcggtcg cccgcgaccc ggccaccggg ttcctcctcg gggcgacgga
     2461 aaagcggaag gacggggccg ccgccggctt ctgattggtc gggcgggccg ctcccacatg
     2521 gcggcggagg cgcggaagcg gggtgtaacc ttcgccccat gagcgtctcc gccgagaagg
     2581 ccgtcccccg cgtctccgag cggggccggc gcgtccccgc ctccccgatc cgaaagctcg
     2641 cctcctacgc cgacgcggcc aagaagcgcg gcgtgaaggt ctaccacctc aacatcggcc
     2701 agcccgacct cgagaccccc gccccgatgc gggaccggct cgcgcagatc aaggacaagg
     2761 tctacgccta caccccgtcg aacggcaccg ccgagtgcct cgacttcctc gtcggctact
     2821 accgcggccg cggcgtggag ctgacgaaga gccaggtcat cacgacgacc ggcgggagcg
     2881 aggcgatcca gttcgccttc atggcctgct gcgaggtcgg cgacgaggcc ctcctcgtcg
     2941 agcccttcta cacgaactac cgcgcgttcg cggcgatggg cggcatcgac ctgaagccga
     3001 tcgtgacccg cgccgaggac ggcttccacc tcccctcgcg cgaggagtgg gagaaggccc
     3061 tcacgccgaa gacgaaggcc gtcctcctct gcaacccgaa caacccgacc gggacggtct
     3121 actcgaagga ggagctcgtc cgggtcgccg agttctgccg cgaccacggc ctcttcctga
     3181 tcgccgacga ggtctaccgc gagttcgtct acgacggacg ccaggtcgag agcgccctgt
     3241 cgctcgaagg gttcgaggac gtcgtcgtcg tcgtcgactc cctctcgaag cgctacagcg
     3301 cctgcggcat ccgcctcggg tgcctcgcca cgcggaacaa ggacgtctac caggcctgcc
     3361 tccacatggc gcaggggcgc ctctccccgc ccggcctcgc gcaggcgatc gcgcccggcg
     3421 ccgcgctcct cggccccgac tacgtcgaag gggtcgtcaa ggagtacgga caccggcgcg
     3481 acatcctcta cgaggagctg acgaagatcc ccgggatctt ctttcgcaag cccgaggggg
     3541 ccttctactt cgtcgcccgg atcccggtga aggacagcga ggacttcgcc cagtggctcc
     3601 tcaccgactt ccagctcgac ggcgcgaccg tcatggtcgc ccccgccgac ggcttctacg
     3661 cgacgcccgg cctcgggaaa gacgaggtga ggatcgccta cgtcctgaag gccgaggacc
     3721 tgaaggccgc ctgccgcgtc ctcgaggcgg cgatcccggc ctaccgcgcc gcgcgaggac
     3781 tctgacccga gacctcgcag agcactctgt tcgcgcgtgt gccggctctt ccgagccggc
     3841 acttgctttt ctgccgtccg ggtcccacct tctcgtcaag ggacagccaa ctccagagag
     3901 ttcgtctcac ctacagacct gatttcccct ggccggcgag gaaccactct ccgccgcccc
     3961 cctccccgcc aggccaggga cgccgggagc gtcgccccat cggcacgctc ccggcagctt
     4021 tctccgagca cgcgacggac ggccggcctc cgaggtaacg tcgggaccgt gctgcgccac
     4081 gtcgcaccct tgatcggact cggccttctc tctgccgcgt gcggcgcccc gcccggtccg
     4141 tcgtccccgc cgcctcgctc cggcccggag gccttccgac ccgttcctcc ccccaggatc
     4201 gcatcggcat ccgcggccgc gacagccgcc gatgcccggc ctctcaaagc gctcgaagcc
     4261 gccggcctct ctcccgtcga gatgaccgcg gccgccgtcg agggcttccc gccgctcccg
     4321 tcctacaccg caaaggtccg gaacgtctcg aaccgcccga tccgccgggt cgtcgccacg
     4381 gtcgtctacc tggacgccgc cggccgcccg atgccggggg aggagcacga cgtctccttc
     4441 ggtagcccgc tgaaggcgat cgacccgggc ctcaccttcg aaagcgtatt cctctctcgc
     4501 gtcgaccgtg cccccgccgt ccgcctcgtc ccgcgtgccg tcacggttct cgagaaggga
     4561 gacggacccg agccgatcga acgggagtgg aagaaccccc attacgacga ggagctgcac
     4621 cgagcctttc ggccctgatc gcgaggcatc ccggcgctcc gggcgcgagg tgcccgcggt
     4681 cgcctctcgc ccggacgccg ctcagagcag ctccacgagc gtcgggagcc gttcccgcag
     4741 ccgcgccgac tcgtccggga gttccgccag gagcacgaag tcggcgaagt cgaagccggg
     4801 ccccacgtcg cacgccacga gggcgtatct cccgagcggc cgcgccgcct gccaggcgcc
     4861 ggccggaacg acggcgagag gctcgttccc ttggctcgcc cgcccgagga cgtggcgcgt
     4921 cgcccccgcg aatcccgcgt cgacgacgag gagctccaga gggtcgccct cgtggaagtg
     4981 ccagacctcg tcggagagga cccggtgcca gcggctcacc tctccccgcg cgaggaggaa
     5041 gaggatcgac gtgagcg
//
LOCUS       DAOTEZ010000326         5030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_12043_length_5030_cov_6.033970, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000326 DAOTEZ010000000
VERSION     DAOTEZ010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12043_length_5030_cov_6.033970"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3727)
                     /locus_tag="P5164_14960"
     CDS             complement(<1..3727)
                     /locus_tag="P5164_14960"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HRY45243.1"
                     /translation="MARGEKKSRSRARRVLLGVVAILVVLAAAAIALLKAPPVGRRLV
                     DAALRKAGDASGLAISARERSLDALEGKLRLGGLVVGLPGEPPFLSVDAAEAELLVGE
                     ALQGRVRLRFVSLTGVRLDLGAPRPASKEPSATELPILSAAQIERFRIEGATVLSGPL
                     PEALRTVALSASASDVRFDGDLRGGKLRLRGETPRIVVERPGGLRLEAKGDVALTASA
                     DGELVLEALHVDGDGLSLSALGGGGLSPEAPLGLRAEATLDPARLAPELQATGSLRIA
                     ARVGGRRAAPTVGATLEGRDLATKALAITLATARVRFADDTLLVESAQADLRDGGRVE
                     GEGRYLLATGDGSWSLRANGLPDTLLARYADPATRERLGIAGATLDAVATVRHGRGEP
                     RPLEVDATATLSRGGGTLAEASADLVARGDAKVDVAATFLPDSPGKRTAEGRVRAASL
                     AGLGSGRIEEGRVALEVPDAAEAVAELGALFPALVPAAPEGIDLAGPLRLDADVSGPI
                     RAPRATIAGRFRPARGGTLALDASADAARGSAAGRLTAAGLGLATFRPGGTGLASADV
                     SFSLSRKARHALVVLDAADVCLSEKAPRVDALHATLALDGPELSILHLAAGSAAVPSP
                     AGPAPARLDASGRLSLDAPFEDADLRALLLAAGLPAEGNAVVRGGVLAFDVPSAGRPG
                     LAGTLAARVPLGALRELPALAGAIPAGLPEGPIELTLDAPGLDACALGPLLPAGTEAP
                     AARGDVRLFATFGLADPLAGTATIEIEGLTLESPAGPVRLRETARLSLAGGRFALAPV
                     TVDGERTSFTVAAGAELLPGARLGGPLESLVAHLSATARGRADAALLTPFLQGGIAKG
                     ELALDASASGPPRALAGRVVVDGTGTRFTWPLAYPTELGDPVLEAELTPGQVELTRAE
                     ALLNGGPLRLSGGLYPGVGATATARFSDVRYRLAYGLASVLSGELTFDQLEDGRRLSG
                     TVVLERGLLERDVDLDREVLALVLAPPESPGTEASFLDTLALDLGITTASGVRIRNNV
                     ADLTASWSRLDVSGTANRPVVRGRIDVERGGLVFAYGQTFRIDRGVVTYSGDPQSDPR
                     LDFVTTSSLEDPSIAAAAGGSDLFASARRSASDATPSDDAAAALAEGLAGYYGNRLAG
                     SLGAALGRVSLSVRPLLLLGETDAAARLTLSRDFSPNVSLAVGIDLKNAQRQTWVVDV
                     HGLRRLPPLSVQAFTEDYGRFGGTLQQRIELGGTRPGA"
     gene            3857..4846
                     /locus_tag="P5164_14965"
     CDS             3857..4846
                     /locus_tag="P5164_14965"
                     /inference="COORDINATES: protein motif:HMM:NF022135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HRY45244.1"
                     /translation="MIALVWLWLGATGATAAQTWLHPVLPESVVKAAPAPRRGGRAAR
                     PAPPARPAPFPTAASGETSFVEVTTGEGYPEPGSAVPPEKIERSFARLGEGRPMPLTG
                     ARVDGPLTRLEATWLGQGLASLGLLLVPEGRTVEAAAFETFLRESGAREALALRAKKK
                     ETRKPARVVTIESARAFAGIVAPPRATRTADPAAGRDEALGLPLELVTGGSPLTLRAG
                     DVLTATLLLDGRPLAGALVRALAPGAEAALEAETDAHGTASIPLEREGRLLLSAAAVR
                     RTEKADRTRGEIWKKADWEVRRTTLELLVLPKAPPKAAPAPTGKRPPAKKPPR"
BASE COUNT          654 a         1806 c         2031 g          539 t
ORIGIN      
        1 cggcgcccgg gcgcgtgccg ccgagctcga tgcgctgctg gagcgtgccg ccgaagcggc
       61 cgtagtcctc cgtgaacgcc tgcaccgaga ggggcggcag gcgccgcagg ccgtggacgt
      121 cgacgaccca ggtctgccgc tgcgcgttct tcaggtcgat cccgaccgcg agagagacgt
      181 tcggcgagaa gtcgcgcgag agcgtgaggc gtgcggcggc gtcggtctcc ccgaggagga
      241 ggagcggccg caccgagagg gagacgcgcc cgagcgccgc tccgagcgat cccgcgagac
      301 ggttcccgta gtagccggcg agcccctcgg cgagcgccgc cgcggcgtcg tccgaggggg
      361 tcgcgtcgga ggcgcttcgg cgcgccgagg cgaagaggtc gctcccgccg gcggccgccg
      421 cgatcgacgg gtcctcgagg gacgaggtcg tcacgaagtc aaggcgcggg tcgctctgcg
      481 ggtcgccgga gtaggtgacg acaccgcggt cgatccggaa ggtctggccg taggcgaaga
      541 cgagcccgcc cctctcgacg tcgatccgcc ccctcacgac gggccggttc gccgtccccg
      601 acacgtcgag gcggctccag gaggcggtca ggtcggcgac gttgttccga atcctcacgc
      661 cggaggcggt cgtgatcccg aggtcgaggg cgagcgtgtc gaggaacgag gcctccgtgc
      721 cgggggactc cggcggcgcg aggacgaggg cgaggacctc gcggtcgagg tcgacgtccc
      781 gctcgaggag gccgcgctcg aggacgacgg tccccgagag cctccgcccg tcctcgagct
      841 ggtcgaacgt cagctctccc gagaggaccg aggcgagccc gtaggcgagc cggtagcgaa
      901 cgtcggagaa ccgggcggtg gcggtcgctc cgacgcccgg gtagagaccg ccggacaggc
      961 ggagcggccc gccgttcagc agcgcttccg cgcgcgtcag ctcgacctgc ccgggcgtca
     1021 gctccgcctc gaggaccggg tcccccagct cggtcgggta ggcgaggggc caggtgaaac
     1081 gcgtccccgt gccgtcgacg acgacgcgcc ccgcgagggc gcggggcggg ccggaggcgc
     1141 tcgcatccag cgcgagctcg cccttcgcga ttccgccctg gaggaacggc gtcaggagag
     1201 ccgcgtcggc ccgtccccgg gccgtcgccg acaggtgcgc gacgaggctc tcgagcggcc
     1261 ccccgagccg tgcgccgggg aggagctccg cgccggcggc cacggtgaag gaggtcctct
     1321 cgccgtcgac cgtcaccggc gcgagcgcga agcggccgcc ggcgagcgag aggcgcgccg
     1381 tctcgcggag ccgaaccggc ccggcgggcg actcgagcgt cagcccctcg atctcgatcg
     1441 tcgccgttcc cgcgagaggg tcggcgaggc cgaacgtggc gaagaggcgc acgtcgccgc
     1501 gcgccgccgg agcttccgtt ccggccggga ggagcggccc gagcgcgcag gcgtcgaggc
     1561 cgggagcgtc gagagtcagc tcgatcgggc cttcggggag ccccgcggga atcgcgccgg
     1621 cgagggcggg cagctcccgg agcgctccga gcggaacgcg agcggcgagc gttccggcga
     1681 ggcccgggcg cccggccgac gggacgtcga aggcgaggac ccccccgcgg acgacggcgt
     1741 tcccctcggc cggaaggccg gcggccagga ggagcgcccg gaggtcggcg tcctcgaacg
     1801 gcgcgtcgag cgagagccgc ccggaggcgt cgaggcgcgc gggcgcgggg ccggccggcg
     1861 acgggaccgc ggcgcttccg gccgcgaggt ggaggatcga gagctcggga ccgtcgaggg
     1921 cgagcgtcgc gtggagcgcg tcgacgcgcg gggccttctc ggagaggcag acgtcggccg
     1981 cgtcgaggac gacgagcgcg tgccgggctt tccgggagag cgagaaggag acgtcggccg
     2041 aggcgagccc cgtcccgccc gggcggaaag tggcgaggcc gaggccggcg gcggtgaggc
     2101 gccccgcggc cgagccgcgc gccgcgtcgg cggacgcgtc cagggcgagc gtcccgccgc
     2161 gcgccggacg gaacctcccg gcgatcgtcg cgcgcggcgc ccggatcgga cccgagacgt
     2221 cggcgtcgag gcggagcggg ccggccaggt cgatcccctc gggcgcggcc gggacgagcg
     2281 cggggaagag cgcgcccagc tccgcgacgg cctctgccgc gtcggggact tcgagggcga
     2341 cgcggccctc ctcgatccgc ccggagccga ggccggcgag agaggcggcg cggacgcgcc
     2401 cttccgccgt tctcttcccg ggagagtccg gcaagaacgt cgcggcgacg tcgaccttcg
     2461 cgtccccccg cgccacgagg tctgcgctgg cctccgcgag cgtccctccg ccgcgggaga
     2521 gggtcgccgt cgcgtcgacc tcgagcggcc gcggctcgcc ccggccgtgc cggaccgtcg
     2581 cgacggcgtc gagcgtcgct cccgcgatcc cgaggcgctc gcgcgtcgcc ggatcggcgt
     2641 atcgggcgag gagcgtgtcg ggaaggccgt tcgcccggag gctccagctg ccgtcgcccg
     2701 tcgcgaggag gtatcgccct tcgccctcga cccggccgcc gtcgcgaagg tcggcctggg
     2761 ccgattcgac gaggagcgtg tcgtccgcga agcggacgcg ggccgtggcg agggtgatcg
     2821 cgagcgcctt cgtcgcgagg tcgcgcccct cgagcgtcgc cccgaccgtc ggtgccgcgc
     2881 ggcgtcctcc cactcttgcg gcgatccgga gcgagccggt cgcctgcagc tccggcgcga
     2941 ggcgggccgg gtcgagggtg gcttccgcgc ggaggccgag cggcgcctcg ggcgagagcc
     3001 ccccgcctcc gagcgcagag agcgagagcc cgtccccgtc gacgtggagc gcctcgagga
     3061 cgagctctcc gtcggccgag gccgtcaggg cgacgtcccc cttcgcttcg aggcgcagcc
     3121 caccgggtcg ttcgacgacg atccgggggg tctctccgcg cagacggagc tttccgccgc
     3181 gcaggtcgcc gtcgaaccga acgtccgacg cggacgccga aagggcgacg gtgcggagcg
     3241 cctcgggcag cgggccggag aggaccgtcg cgccttcgat ccggaaccgc tcgatctggg
     3301 ccgcggagag gatgggaagc tcggtggccg acggctcctt cgacgccggc cggggagcgc
     3361 cgagatcaag gcggactccg gtgagcgaga cgaagcggag gcgcacccgt ccctggagcg
     3421 cctcgccgac gaggagctcg gcctcggccg cgtcgacgga gaggaagggc ggctctcccg
     3481 gcaggccgac gacgaggccg ccgaggcgaa gcttcccttc gagcgcgtcg agcgagcgct
     3541 cgcgcgccga gatcgcgagg cccgacgcgt ccccggcctt ccgcagggcc gcgtcgacga
     3601 gccgccgacc gacgggcggc gccttcagga gggcgatcgc cgcggcggcg aggacgacga
     3661 ggatcgcaac gaccccgagg aggacgcgcc gggcgcggga ccgactcttc ttctcgccgc
     3721 gggccacggt tcgattctag gcgggtgcgg tcgagccctc gggtggaggc gggccgccct
     3781 agaatccgcg gttcggatgg gaggggagat gcggagagag ccgacgcggg aggcgacgat
     3841 cgggaggctc gggcgcgtga ttgccctcgt ctggctgtgg ctcggcgcga ccggggcgac
     3901 ggcggcccag acgtggctcc acccggtcct tccggagagc gtcgtgaaag cggctccggc
     3961 cccccgccgc ggcggaaggg ccgcgcgtcc cgccccgccg gcgcggccgg cgcccttccc
     4021 gacggccgcg tcgggagaga cctccttcgt cgaggtgacg acgggggagg gctaccccga
     4081 gccggggagc gcggtccccc ccgagaagat cgaacgctcc ttcgcccggc tcggcgaggg
     4141 gcggccgatg cccctcaccg gcgcgcgggt cgatggcccc ctcacgcgtc tcgaggcgac
     4201 gtggctcggc cagggcctcg cgtcgctcgg tctcctcctc gttccggagg gccggacggt
     4261 ggaggcggcg gcgttcgaga cgttcctccg cgagtcgggc gcgcgcgagg cgctcgcgct
     4321 ccgagcgaag aagaaggaga cgaggaagcc ggcgcgcgtc gtgacgatcg agagcgcgcg
     4381 ggccttcgcc gggatcgtcg cgccgcctcg cgcgacgcgg acggcggacc cggcggcggg
     4441 gcgcgacgag gctctgggac tcccgctcga gctcgtgacc ggcggttcgc cgctcacgct
     4501 gcgcgcgggg gacgtcctga cggcgacgct tctcctcgac ggccggccgc tcgccggcgc
     4561 cctcgtccgc gcgctcgccc cgggggcgga ggcggcgctc gaggccgaga cggacgcgca
     4621 cggcaccgcc tcgatcccgc tcgagcgcga gggacggctc ctcctgtcgg ccgctgctgt
     4681 ccgccggacg gagaaggccg accggacgcg cggggagatc tggaagaagg ccgactggga
     4741 ggtccggcgg acgaccctcg agctgctcgt cctcccgaag gccccgccga aggcggctcc
     4801 cgcgccgacg gggaagcggc ctccggcgaa gaagccgccg cgctgacgga ggctcgtcag
     4861 gcgggacggt cgatcgcgcg ggccagccgc gcagccccct cgcggaagaa gtcctctccg
     4921 gggaggaggg agacgacgag atacgcctcg ttcgggaagt cgaagaaata cccggggtgg
     4981 acgacgaggt cctcctcgtc gaggagcccg aggacgaaat actcctcccg
//
LOCUS       DAOTEZ010000327         4998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_12153_length_4998_cov_4.828040, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000327 DAOTEZ010000000
VERSION     DAOTEZ010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12153_length_4998_cov_4.828040"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            404..>2974
                     /gene="dnaE"
                     /locus_tag="P5164_14970"
     CDS             404..>2974
                     /gene="dnaE"
                     /locus_tag="P5164_14970"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRY45245.1"
                     /translation="MSSDFVHLHLHTQYSLLDGANRTKDLIEHVLKSGMSAMAVTDHG
                     NMFGAFDIQMEAVKKGIKPILGVEAYIAPGSRHDRETVKTVDGEGNSYHLTLLAESPE
                     GYRNLSYLVSEAFLSGFYYKPRMDWELLEKHHEGLVALSGCVKSEVSQRLLAGDFAGA
                     KETALRYEGLFGKDHYYIELMDHGLPIQRQLMPELLRLSRETGIPVVATNDAHYLRRV
                     DSEAQDVLLCIGMGKTVNDSRRMKFYNSEFYVKDPGEMQDAFREWSLEAVRNSVAVAD
                     RVAPKVIIDTSYKIPTFPLPEEARGPDEYLEELARAGLERRLAPIAELLAAGRTKHPR
                     KDYDDRLEWELGVIRRLGFSGYFLIVWDFIKYAKDNGIPVGPGRGSAAGSLVAWTLGI
                     TDVDPLRYDLLFERFLNPDRVSMPDIDVDLCERRRGEVIEYVRHKYGKENVGQIITFN
                     SMKARAVIRDVGRALDVPLAEVNKLCAMIPANPGKPTTLSEARKSVKELSDTLAGNEG
                     YRRLFDLAERLEGVSRHAGVHAAGVLIAPRPLVEYLPLYKNNNDEITTQFEMKSVEKM
                     GLLKMDFLGLITLTILDDAVKSVVRRTGRSLDLAEIPLNDPEVFRLFSEGRTDGIFQF
                     ESSGMRDLLRRVQPSVFSDLAALNALYRPGALDAGTVEVYIERRRGKRFDYPLPQLEP
                     ILKDTYGVLVYQEQVMLAARAVAKYTPGEADLLRKAIGKKDEAALQAEGEKFVKKAVA
                     EGVPKKKAEELWELILPFGRYGFNKSHSVVYALLAYRTAWMKVHYPVEFMAAVLTAAS
                     GKSEDVVKYVNACREMKIPVLPPDVNASQLSFTPDGESIRFGLGAVKGVGEGAVASVL
                     G"
     assembly_gap    2975..3074
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3075..4098
                     /locus_tag="P5164_14975"
     CDS             <3075..4098
                     /locus_tag="P5164_14975"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45246.1"
                     /translation="GLGAVKGVGEGAVASVLGARAADGPFVSLTDFCCRVDLRLNNRK
                     VIEALIRSGSFDSLKQSRASLAAGVDSAMEVAAAERKSRESGQGGLFADESAVDHADR
                     FPALPEWSPDERIRFEKETLGFYITGHPLARFEDEIALFGDVTAERAGERVDQNVRIV
                     ALLASLKKTQIKKGQNEGKMMAKGVLEDLTGSVPVTVFAALLERLGSWLSEGTPVLVS
                     GVVRSSSAPGAPEPEGEGAPVPVEIIAREIQALEGMREAAATELRLVTPLPGTLDETL
                     ASVKEILESSPGSVPVFLDVRRAGHWEARVRTAGRLAVRPTPELTRGLERLLGPGAVR
                     YVYAHT"
     gene            4095..4991
                     /locus_tag="P5164_14980"
     CDS             4095..4991
                     /locus_tag="P5164_14980"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45247.1"
                     /translation="MTTAPIRPLDSLEVVARRARKAGAQAYLVGGAVRDAALGRTPTD
                     LDVALEGPASTTEKLVHALADEGFMCEARHARFGTATLLSPSGTRIDLAATREEAYPV
                     PGALPVVRCGAPIERDLARRDFSIHAMALALGPDGPEGRAIDPFDGAGDLSRRRIRLL
                     HERSLADDPTRVLRAARYAARLGFSLDAGFVAAMQHSVAEGSFGTISGDRLRRALEEV
                     LSEENPHVALDLLLRLGVPAAIVPGWEGAHFPSPEETTTTASVPDRWRRLLAPAPEAL
                     RAQVARRLAFSRALRAATGCGR"
BASE COUNT          758 a         1638 c         1776 g          726 t
ORIGIN      
        1 tgccgcggcg cgtagctgcg gcgaagggtc gccttcgctc cgcgcggcgt caggggcgca
       61 tgccgataac cgcggttatc ggccccggag cggctgaagt cccgctccgc tgacgctccg
      121 cgggggcgcc cctcccgcct ccgcgtcgcg ccttcggcgc gccgtttgac ataaccgaag
      181 gctatgtcaa accgccgggg gcgagcgggg ctcgctacgc tcggtcgctg aagggcctga
      241 tcacgcggcg gcgtcggcta tgccgacgcg ggttcgaggg cgccgatgac ctcgccccgc
      301 cgaaccgatc caccggcccg ggcatccggc cctggggagc ctgcaggcgg tcaaaccgga
      361 gacccggccg gcgctcgggc cgagacacta gaatccagcc ggcatgtcgt ccgatttcgt
      421 ccatctgcac ctccacaccc agtactccct tctcgacgga gcgaaccgga cgaaggacct
      481 gatcgagcac gtcctgaaga gcgggatgag cgcgatggcg gtcacggacc acgggaacat
      541 gttcggggcc ttcgacatcc agatggaggc ggtgaagaag gggatcaagc cgatcctcgg
      601 cgtggaggcc tacatcgcgc cgggctcgcg ccacgaccgc gagaccgtga agacggtcga
      661 cggggagggg aacagctacc acctcacgct gctcgccgag agcccggagg gctaccggaa
      721 cctctcgtat ctcgtctccg aggcgttcct gagcggcttc tactacaaac cccggatgga
      781 ctgggagctt ctcgagaagc accacgaagg cctcgtcgcc ctctccgggt gcgtcaagtc
      841 ggaggtctcg cagcggctcc tcgcgggcga cttcgccggt gcgaaggaga cggcgctgag
      901 gtacgagggg ctgttcggga aggaccacta ctacatcgag ctgatggacc acgggctccc
      961 gatccagcgg cagctgatgc cggagctcct gcgactctcg cgcgagacgg ggatcccggt
     1021 ggtcgcgacg aacgacgccc actaccttcg ccgggtcgac tccgaggcgc aggacgtcct
     1081 cctctgcatc gggatgggga agacggtgaa cgactcccgg cggatgaagt tctacaactc
     1141 ggagttctac gtgaaggacc ccggggagat gcaggacgcg ttccgggagt ggtcgctgga
     1201 ggcggtgcgg aactccgtgg cggtcgccga ccgcgtcgcg ccgaaggtca tcatcgacac
     1261 gagctacaag atcccgacgt tcccgctgcc cgaggaggcg cgcggccccg acgagtacct
     1321 cgaggagctc gcgcgggccg gcctggagcg gcgcctcgcg ccgatcgccg agctcctcgc
     1381 ggcgggccgg acgaagcacc cgcggaagga ctacgacgac cggctcgagt gggagctggg
     1441 cgtcatccgg aggctggggt tctcgggcta cttcctgatc gtctgggact tcatcaagta
     1501 cgcgaaggac aacggcatcc ccgtcggacc gggacgggga agcgccgcgg gctcgctcgt
     1561 cgcgtggacc ctcggcatca ccgacgtcga cccgctccgg tacgacctcc tgttcgagcg
     1621 gttcctcaac cccgaccgcg tctcgatgcc cgacatcgac gtcgatctct gcgagcgccg
     1681 gcggggcgag gtcatcgagt acgtccggca caagtacggc aaggagaacg tcggccagat
     1741 catcacgttc aactcgatga aggcgcgcgc cgtcatccgc gacgtcgggc gcgccctgga
     1801 cgtccccctc gccgaggtga acaagctctg cgcgatgatc ccggcgaacc ccgggaagcc
     1861 gacgacgctg tcggaggcgc ggaagagcgt caaggagctc tccgacacgc tcgccgggaa
     1921 cgaaggctac cggcgcctgt tcgacctggc cgagcggctc gagggggtct cgcggcacgc
     1981 cggcgtccac gccgccggcg tgctcatcgc cccgcgcccg ctcgtcgagt acctgccgct
     2041 ctacaagaac aacaacgacg agatcacgac ccagttcgag atgaagtccg tggagaagat
     2101 gggtctcctg aagatggact tcctcgggct gatcacgctc accatcctcg acgacgctgt
     2161 caagtccgtc gtgaggagaa cggggaggag cctcgacctc gctgagatcc cgctgaacga
     2221 cccggaggtc ttccggctct tctccgaagg acggacggac gggatcttcc agttcgagtc
     2281 gtcggggatg cgggacctcc tccgtcgcgt ccagccgagc gtcttcagcg acctcgccgc
     2341 gctcaacgcc ctctaccgac cgggtgcgct cgacgccggg acggtggagg tctacatcga
     2401 gaggcgccgg gggaagaggt tcgactaccc gctgccgcag ctcgagccga tcctgaagga
     2461 cacgtacgga gtcctcgtct accaggagca ggtcatgctc gcggcgcggg ccgtcgcgaa
     2521 gtacacgccc ggcgaggcgg acctgctccg gaaggcgatc gggaagaagg acgaggccgc
     2581 gctccaggcc gaaggcgaga agttcgtcaa gaaggccgtg gcggaaggag tgccgaagaa
     2641 gaaggccgag gagctctggg agctgatcct tcccttcgga cggtacggct tcaacaagtc
     2701 gcactcggtg gtctacgccc tcctcgcgta ccggaccgca tggatgaagg tccactaccc
     2761 ggtcgagttc atggcggcgg tgctgacggc ggcgtccggg aagtccgagg acgtcgtgaa
     2821 gtacgtgaac gcctgccgcg agatgaagat tccggtcctc ccgccggacg tgaacgcctc
     2881 gcagctctcg ttcacgccgg acggcgagtc gatccggttc gggctgggag cggtgaaggg
     2941 ggttggcgag ggggcggtgg cttcggtcct cgggnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnncgggct gggagcggtg aagggggttg gcgagggggc ggtggcttcg
     3121 gtcctcgggg cgcgcgctgc cgacgggccg ttcgtttcgc tcaccgattt ctgctgccgg
     3181 gtcgacctgc gcctgaacaa ccggaaggtg atcgaggcgc tgatccgctc gggctccttc
     3241 gactcgctca agcagagccg ggcgagcctc gcggccggag tcgactccgc catggaggtc
     3301 gcggcggcgg agaggaagtc gcgggagtcc ggacaggggg ggctcttcgc ggatgaatcc
     3361 gccgtcgacc acgcggaccg gttcccggcg cttccggagt ggtcgcccga cgagcggatt
     3421 cgattcgaga aggagaccct cggcttctac ataaccgggc atccgctcgc gcggttcgag
     3481 gacgagatcg ctctcttcgg cgacgtgacg gccgagcggg ccggcgaacg cgtcgaccag
     3541 aacgtgcgca tcgtcgccct cctcgcctcg ctgaagaaga cgcagatcaa gaaggggcag
     3601 aacgagggga agatgatggc gaaaggggtg ctcgaggacc tcaccgggtc ggtgccggtg
     3661 acggtcttcg ccgcgctcct cgagcgcctc ggatcgtggc tctcggaggg gacgccggtc
     3721 ctcgtctcgg gcgtcgtccg gtcttcgtcc gctcccggcg cacccgagcc ggaaggggag
     3781 ggggcgcccg tgccggtcga gatcatcgcc cgcgagatcc aggcgctcga agggatgcgc
     3841 gaggcggcgg ccaccgagct ccgcctcgtc acccctctgc cggggacgct ggacgagacg
     3901 ctcgcgtcgg tgaaggagat cctggagtcg tctcccgggt cggtccccgt ctttctcgac
     3961 gtccggaggg ccgggcactg ggaggcacgg gtgcggaccg ccggccggct ggcggtccgg
     4021 ccgacgccgg agctgacgcg cggcctcgag cggcttctcg ggcccggcgc cgtccggtac
     4081 gtctacgccc acacgtgacg actgcgccca tccggccgct cgactcgctc gaggtggtgg
     4141 cgcgtcgggc gcggaaggcg ggcgcgcagg cctatctcgt cggaggggcg gtgcgggacg
     4201 cggcgctcgg gagaacgccg acggacctgg atgtggcgct cgagggcccg gcgtcgacga
     4261 ccgagaagct cgtgcacgcg ctcgccgacg agggcttcat gtgcgaggcg cggcacgcga
     4321 ggttcgggac cgcgacgctc ctctcgccgt cgggcacgag gattgacctg gccgcgacgc
     4381 gggaggaggc ctatcccgtt cccggcgcgc tcccggtcgt gcggtgcggg gccccgatcg
     4441 agcgcgacct ggcccggcgg gacttctcga tccacgcgat ggcgcttgcg ctcggaccgg
     4501 acgggccgga gggcagagcg atcgacccgt tcgacggcgc cggcgatctc tcgcgccgtc
     4561 gcatccgtct ccttcatgaa aggtcgctgg cggacgatcc gacccgcgtg ctccgggcag
     4621 cgcgctacgc cgcccggctc ggcttctcgc tcgacgccgg gttcgttgct gcgatgcagc
     4681 attcggtcgc cgaaggctcg ttcgggacga tctccggcga tcgcctccgt cgcgcgctcg
     4741 aggaggtgct ctcggaggag aatccgcacg tggccctgga tctcctcttg cggctcggcg
     4801 tcccggcggc gatcgtcccg ggctgggagg gagcgcattt cccgtcgccc gaagagacga
     4861 cgaccacggc gtcggtcccg gaccggtggc ggcgcctcct cgcgccggcc ccggaagcgc
     4921 ttcgagcgca ggtcgcgcgg cgtctcgcgt tctcgcgcgc gcttcgcgcc gccacgggat
     4981 gcggacgttg agacgggc
//
LOCUS       DAOTEZ010000328         4977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_12218_length_4977_cov_5.712312, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000328 DAOTEZ010000000
VERSION     DAOTEZ010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12218_length_4977_cov_5.712312"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /gene="acnA"
                     /locus_tag="P5164_14985"
     CDS             <1..695
                     /gene="acnA"
                     /locus_tag="P5164_14985"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240746.1"
                     /note="catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HRY45248.1"
                     /translation="RCLALLGDSITTDHISPAGNIAKTSPAAKYLGDNGVKPADFNQY
                     GARRGNHEVMMRGTFANIRLKNLMLPGTEGGVTVHVPTGEPMSIYDAAMKYQAEGTPL
                     VVFAGAEYGTGSSRDWAAKGTMLLGVKAVIAKSFERIHRSNLAGMGVLPLQFAPGEDA
                     QTLGLTGKETFDVAGVAEGLTPHKKLTVTATAEDGTKKTFTATLRLDTPNEVDYYRHG
                     GILQFVLRQMTA"
     gene            808..2277
                     /locus_tag="P5164_14990"
     CDS             808..2277
                     /locus_tag="P5164_14990"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRY45249.1"
                     /translation="MGLFSDGPKGDSPALREAIEAATKRLKEDPGDAAAVLRLADALT
                     ASGRKADAVRALNRYGPVVQSKGRLEEAIAIYKKATQLDPESELTSSTFLSYLQLKKL
                     VEAEREERAEAPPVVRRPPSGFYTRPGPDPPPPSGAFPKPVAGPPASGSFPLPAPAAS
                     PPPSGSFALPQQAAAPPPSGSFPKPPTEPPADEPMPAPQAASDWSRKKEAVHEAQAGI
                     PLLRDIPPVLLDLVLQKINLVTLAPGEVLFREGSEGSSVFFVVQGSLDVAVRSDAGED
                     VLLRIARNGEAIGEASFLTSLPRYATATAREPANLLELDHNALVPLARRHRPLAEALN
                     RLYDERVLTAALARSRVFGALPEAERRLLTRKLTPVSVSAGTVVVREGRPADAAFVVK
                     RGAFRVTFRSDGREVAVALLRPHELFGDLGDESATPQAETVAAVVDSELLCLAPLELA
                     AFRSRHAGLATALDALRFERAERCVAALRASRRPSPSSR"
     gene            2364..2768
                     /locus_tag="P5164_14995"
     CDS             2364..2768
                     /locus_tag="P5164_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015006685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HRY45250.1"
                     /translation="MAGIKGIEGIGETYGEKMKALGIRSKAALLKAGATKKGRAELAA
                     KAGISEKLILEWVNRADLYRVTGIGTQYADLLENAGVDTVVELAGRKAENLVEKMRQV
                     NAEKKLVRLLPALSKVQGWIAEAKTLPRAVTY"
     gene            2828..4051
                     /locus_tag="P5164_15000"
     CDS             2828..4051
                     /locus_tag="P5164_15000"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRY45251.1"
                     /translation="MVRFFLASALAVLPALSLQAEVRSVRLVRQGAGLSGGRLEAGVT
                     VRADGLHAERNAAYVSPPLDAGLAFSAIGPHWKAAPGAEIEIAVSADGLTWGRWIPAP
                     SEETIDSLREDGTSRLFGGDALGPLVFVSPDSRWVRYRVRLPTRADGEPSLSRIVFHL
                     IDPGEDEAEGPPAIDGTARDARETREPDRSEAPAPRRARAPAPEEPEPVSAAPRPSIL
                     SRSAWGARAPKYAYSYTRAGHVGIHHTASVDDFAAPTRAECAGRLRAIQAFHMDTNGW
                     NDIGYAWVVCRHGDVFQGREDDDDTTDVQGAHDGFNRGSTGLALLGYFHPPYDQRPTE
                     EQLSALVDLIAWISGLRGIDPAGRSLYEAFGAEVDNVYGHREVKETSCPGDHLFARKE
                     LLRAAALEKLLVGAL"
     gene            complement(4082..>4977)
                     /locus_tag="P5164_15005"
     CDS             complement(4082..>4977)
                     /locus_tag="P5164_15005"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HRY45252.1"
                     /translation="AMVRNEIAAFAMRIDRLDWMTEETKAKAKAKLAVLKVGVGYPDR
                     WRDYSGLEVVAGDALGNARRASLFELRRNLGKLGRPVDRGEWMMNPQLVNAVNLPAMN
                     AMNFPAAILAPPDFDPTRPEVMDYGAAGATIGHEISHSFDDQGALFDAEGRLRNWWTK
                     EDFAHFAASAEKLVAQYDAYRPFPDLGVNGRLTVSENIADVAGLAVAYDAYRLALGGR
                     EAPTVHGLTGDQQFFLSFAQSWRTKTREAALRQRIVTDAHAPAEYRADCVRNLDAWYA
                     AFDVKPGQRLWLAPGDRVKVW"
BASE COUNT          706 a         1925 c         1688 g          658 t
ORIGIN      
        1 cccgctgcct cgccctcctc ggcgactcca tcaccaccga ccacatctcc cccgccggca
       61 acatcgcgaa gacctcaccg gcggcgaagt acctgggcga caacggcgtc aagcccgccg
      121 acttcaacca gtacggcgcc cgccgcggca accacgaagt gatgatgcgc ggcaccttcg
      181 ccaacatccg cctgaagaac ctgatgctcc cggggaccga gggcggcgtc accgtccacg
      241 tcccgaccgg cgagccgatg tcgatctacg acgccgcgat gaagtaccag gccgagggga
      301 cgcccctcgt cgtcttcgcc ggcgccgagt acggcaccgg ctcctcgcgc gactgggccg
      361 cgaaggggac gatgctcctc ggcgtgaagg ccgtgatcgc gaagtcgttc gagcggatcc
      421 accgctcgaa cctcgccggc atgggggtcc tcccgctcca gttcgccccg ggcgaggacg
      481 cccagacgct cggcctcacc gggaaggaga ccttcgacgt cgccggcgtc gccgagggcc
      541 tgacgccgca caagaagctc accgtcacgg cgaccgcgga ggacggcacg aagaagacct
      601 tcaccgccac cctccgcctc gacaccccga acgaggtcga ctactaccgc cacggcggca
      661 ttctccagtt cgtcctccgg cagatgacgg cgtaggcgcg ccgcccgaac cggtcacggc
      721 acgagggccg cgggagcgat cccgcggctc tctgctgttc cgccgtctcg cttgcgctcc
      781 ggcctcacac gcgtacagtt cgtagggatg ggcctcttct cggacggtcc gaagggcgac
      841 tcgcccgcgc tgcgggaggc aatcgaggcg gcgacgaagc gcctgaagga ggacccgggc
      901 gacgccgccg ccgtgctgcg cctcgccgac gcgctcaccg cgagcggccg gaaggccgac
      961 gccgtccgcg ccctgaaccg ctacggaccg gtcgtccagt cgaagggacg cctcgaggag
     1021 gcgatcgcca tctacaagaa ggcgacgcag ctcgaccccg agagcgagct cacctcctcg
     1081 acgttcctct cctatctcca gctgaagaag ctcgtggagg cagagcggga ggagagggcg
     1141 gaagcgcctc ccgtcgtccg ccgccccccg tccggcttct acacccgccc cggcccggac
     1201 cctccgccgc cgagcggcgc cttcccgaag ccggtggccg gcccgcccgc gagcgggtcg
     1261 ttcccgctcc ccgcgcccgc cgcctcgcca ccgccgagcg ggtcgttcgc gttgccgcag
     1321 caggccgcgg caccgccgcc gagcggctcc tttccgaagc cgccgacgga gccgcccgcc
     1381 gacgagccga tgccggcccc ccaggcggcg tcggactgga gccgcaagaa ggaggccgtg
     1441 cacgaggcgc aggccgggat ccctctcctt cgcgacatcc cgcccgtcct cctcgacctc
     1501 gtcctgcaga agatcaacct cgtcacgctc gctccgggcg aggtcctctt ccgcgaggga
     1561 agcgaaggga gctccgtctt cttcgtcgtc cagggctctc tcgacgtcgc cgtccggagc
     1621 gacgcgggag aggacgtcct gctccggatc gcccgcaacg gcgaggcgat cggggaggcc
     1681 tccttcctca cgagcctccc gcgctacgcc accgcgacgg cccgcgagcc cgcgaacctc
     1741 ctcgagctcg accacaacgc cctcgtgccg ctcgcccgca ggcaccggcc gctcgccgag
     1801 gcgctgaacc ggctctacga cgagcgcgtc ctgacggccg cgctcgcccg gtcgagggtc
     1861 ttcggagcgc tgcccgaggc cgagcgccgc ctgctgaccc ggaagctcac tcccgtctcg
     1921 gtctccgccg ggacggtcgt cgttcgggag ggccggccgg ccgacgcggc cttcgtcgtg
     1981 aagcgcggcg cgtttcgcgt cacgttccgg tcggacggcc gggaggtcgc cgtcgcgctc
     2041 ctccgcccgc acgagctctt cggcgacctc ggcgacgaga gcgcgacgcc gcaggccgaa
     2101 accgtcgccg ccgtcgtcga ctcggagctg ctctgcctcg cgcccctgga gctcgcggca
     2161 ttccgctcgc gccacgcggg gctcgcgacg gccctcgacg cgctccggtt cgagcgggcc
     2221 gagcgctgcg tggcggcgct ccgggcctcc cgccgcccgt cgccctcctc ccgctgaccc
     2281 cctgcgccgg ccgaaacagg ggccgccggc aggcgcgaac gagcctaaac tgcccgatgc
     2341 agcacagcac gaggaggtgc cctatggcgg ggatcaaggg gatcgaggga atcggcgaga
     2401 cgtacggcga gaagatgaag gcgctcggca tccgctcgaa ggccgccctg ctgaaggccg
     2461 gcgcgacgaa gaaggggcgc gccgagctcg cggcgaaggc cgggatctcc gagaagctca
     2521 tcctcgagtg ggtcaaccgc gccgacctct accgcgtcac ggggatcggg acccagtacg
     2581 ccgacctcct cgagaacgcg ggcgtcgaca ccgtcgtcga gctcgccggc cggaaggcgg
     2641 agaacctcgt cgagaagatg aggcaggtca acgccgagaa gaagctcgtc cggctcctcc
     2701 ccgcgctctc gaaggtgcag gggtggatcg ccgaggcgaa gacgctgccg cgggccgtca
     2761 cgtactgacc acaggccttt cggccgagcc cgccccgacg gagacggacg gccggaggca
     2821 ccgacggatg gtccgtttct tcctcgcttc ggctctggcg gtccttccgg ccctctccct
     2881 tcaagccgag gtccgctccg tccgcctcgt ccgccaggga gccggcctct ccggaggccg
     2941 gctcgaggca ggcgtcacgg ttcgagccga cggccttcac gcggagcgga acgcggccta
     3001 cgtgtcgccc cctctggacg ccggcctcgc cttctccgcg atcggcccgc actggaaggc
     3061 ggcgccgggc gccgagatcg agatcgccgt cagcgcggac ggcctcacct gggggcgctg
     3121 gatcccggcg ccatccgagg agacgatcga ctcgctccgt gaggacggca cgtcccgtct
     3181 cttcggcgga gacgctctcg gcccgctcgt cttcgtctcc cccgacagcc gatgggtccg
     3241 ctaccgcgtc cgcctgccga cccgcgcgga cggcgagccg tcgctctccc gaatcgtctt
     3301 ccacctcatc gacccgggag aggacgaggc cgagggcccc ccggcgatcg acggcacggc
     3361 ccgggacgct cgcgagaccc gggaaccgga tcgttccgag gcgccggcgc cccgccgcgc
     3421 gcgggccccc gcgcccgaag agcccgagcc ggtctcggca gctccgcgcc cgtcgatcct
     3481 cagccgcagc gcctggggcg cgcgagcgcc gaagtacgcc tactcctaca cgcgggccgg
     3541 acacgtcggg atccaccaca cggcctccgt cgacgacttc gcggcgccga cccgcgccga
     3601 gtgcgccggc cgcctgcgcg cgatccaggc gttccacatg gacacgaacg gctggaacga
     3661 catcggctac gcgtgggtcg tctgccgtca cggcgacgtc ttccaggggc gcgaggacga
     3721 cgacgacacg accgacgtcc agggggcgca cgacggcttc aaccgcggga gcacgggcct
     3781 cgcgctcctc ggctacttcc acccgccgta cgaccagcgc ccgacggagg agcagctttc
     3841 ggccctcgtc gacctgatcg cgtggatctc cggcctccgc gggatcgacc cggccggccg
     3901 gagcctctac gaggccttcg gcgccgaggt cgacaacgtc tacggccacc gcgaggtcaa
     3961 ggagacgagc tgcccgggcg accacctctt cgcccggaag gagctcctgc gcgccgccgc
     4021 gctcgagaag ctcctcgtcg gcgcgctctg acccgggcgg gccgtccggc gcggcagccc
     4081 gctaccagac cttcacgcgg tcgccggggg cgagccagag cctctgcccc ggcttcacgt
     4141 cgaacgcggc gtaccaggcg tcgaggttcc tcacgcagtc cgcgcggtac tcggcggggg
     4201 cgtgcgcgtc ggtgacgatc ctctgccgga gcgcggcctc ccgcgtcttc gtccgccagc
     4261 tctgcgcgaa gctcaggaag aactgctggt cgcccgtcag gccgtggacc gtcggcgcct
     4321 cgcggccgcc gagggcgagc cggtaggcgt cgtaggcgac cgcgaggccg gcgacgtcgg
     4381 cgatgttctc gctgacggtg agccggccgt tcacgccgag gtcggggaac gggcggtagg
     4441 cgtcgtactg cgcgacgagc ttctccgccg aggccgcgaa gtgagcgaag tcctccttcg
     4501 tccaccagtt cctcaggcgc ccctcggcgt cgaagagggc cccctggtcg tcgaagctgt
     4561 ggctgatctc gtggccgatc gtcgcacccg cggcgccgta gtccatcacc tcgggcctcg
     4621 tggggtcgaa gtcgggcggg gcgaggatcg cggccgggaa gttcatcgcg ttcatcgccg
     4681 ggaggttcac ggcgttcacg agctgcgggt tcatcatcca ctcgccccgg tcgacgggcc
     4741 ggccgagctt cccgaggttg cgccggagct cgaagagcga ggcccgccgg gcgttcccga
     4801 gggcgtcgcc cgcgacgacc tcgaggccgg aatagtcgcg ccaccggtcc gggtagccga
     4861 cgccgacctt caggaccgcg agcttcgctt tcgccttcgc cttcgtctcc tcggtcatcc
     4921 agtccagccg gtcgatcctc atcgcgaacg ccgcgatctc gtttcgcacc atcgccc
//
LOCUS       DAOTEZ010000329         4974 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_12230_length_4974_cov_6.826387, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000329 DAOTEZ010000000
VERSION     DAOTEZ010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12230_length_4974_cov_6.826387"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="P5164_15010"
     CDS             <1..192
                     /locus_tag="P5164_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HRY45253.1"
                     /translation="LGGRECSAYPACGPDVTRAEGKYMAIPVDAAHVDGNLVTAPAWP
                     AHPAWLAAFLKVLGTKIEP"
     gene            303..2654
                     /locus_tag="P5164_15015"
     CDS             303..2654
                     /locus_tag="P5164_15015"
                     /inference="COORDINATES: protein motif:HMM:NF020928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HRY45254.1"
                     /translation="MNFESWLQGKHPGIPPRSAAAVLALAAEGATVPFIARYRKEATG
                     GLDEVAIQAILDTKEAWDETVKRQAFIVGEIEAQGKLTPELKERLLATFDLATLEDLY
                     LPYKKKRKTKAVIAREAGLQPLADWLWDVGHGAAAPEPPEAKAAAYLNAEAGFADPPA
                     ALQGATEILVERLSETEELRQYVRREAFERGGVASTKGEEAKPASKYEMYFDFRDSVA
                     ALMKPEASHRYLAMRRGWLEKELTLSLGDAPKHPGFEERLLAEFERAAGAGAATPAQP
                     YLRKAARFALKAHVLLSIEAEVHKALKEVADEAAIHVFAENVRKVLLAAPFGSKAVLG
                     VDPGIRTGCKLAVVDASGKFVASTVVYLKGESGLANARTAITECVKTQAIRCVAVGNG
                     TGGRETEAFVKETLRDAGLEVPVVLVNESGASVYSASETAREEFPDLDLTIRGAISIA
                     RRLQDPLAELVKVDPKSIGVGQYQHDVSPHALKKRLDQVVDSAVNQVGVNLNTASPHL
                     LAHVSGIGPALGKAIVARRETKGLFTSRQELLDVPRFSKKTFEQAAGFLRIPGGANPL
                     DATAVHPERYEALGAFAKGVGRELVDLLGPGVEIVKRSKELRESLGTFTFEDVVKELE
                     KPGRDPRETFVPFAFREGVNAVSDLVPGMICPGIVTNVTNFGAFVDVGVHQDGLVHIS
                     QLSSQRFVKDPREVVNPGDRVQVKVLSVDLARQRISLTMRLGEPAAPPAREGPRPEGA
                     RGGASDGRRDGPAGAGGGRPSARPEPPKPEPDRRWAALANLKK"
     gene            2701..3285
                     /locus_tag="P5164_15020"
     CDS             2701..3285
                     /locus_tag="P5164_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010808965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRY45255.1"
                     /translation="MKTLCSALVLLPLLAAFTVSAQSPSPRVAVKTSMGSFTIELHEK
                     EAPETVRNFLAYVDEGFYAGTIFHRVIEDFMIQGGGLDREMSRKKTRDPIANEAMNKL
                     KNRAGTVAMARTSAPNSATSQFFVNVVDNPMLDYRDTSAEGIGYCVFGKVVEGMEVVE
                     KIRSVPTGVKNGMRDVPTTPVVIESITRVKADAP"
     gene            complement(3373..4683)
                     /locus_tag="P5164_15025"
     CDS             complement(3373..4683)
                     /locus_tag="P5164_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013887138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRY45256.1"
                     /translation="MGHATARGGFRDLVDRLNRLPQGAPPSDLLYRILALLFTEREAG
                     LVALLPVKPFTVATAARAWKTTEAEARKTLDALSSRALLLDAVRPDGETRWVLPPPMA
                     GFFEFSMMRVRDDVDQKALAELFYEYLNVEEDFIRELFARGETQLGRTFVNEDALPAP
                     EALEVLDWERASEVARTASHAAVGVCYCRHKMAHVGRACDAPLEVCLTFGNTADSLAR
                     HGFARRIDSAEALDLLAKGRGLGLVQFGENVRERVAFVCSCCGCCCEALLAQKRFGAH
                     RPVHTTGFLPEVDAERCSGCGACAKACPVDAMGLRPAGDPLPARRVASPSVEACLGCG
                     VCVPACPRGALALVRRGARVIPPKDTVQRVVRMAIERGNLADLIFDDRALASHRVLGP
                     LLGAVLRLPPLERALAREQVKSVWLERAAELWEKRHGGAIGAAG"
BASE COUNT          767 a         1732 c         1845 g          630 t
ORIGIN      
        1 ctcggcgggc gcgagtgcag cgcctacccg gcctgcggcc ccgacgtgac gcgcgccgag
       61 gggaagtaca tggcgatccc ggtcgacgcg gcgcacgtcg acgggaacct cgtcacggcc
      121 cccgcctggc ccgcccaccc ggcctggctc gccgcgttcc tgaaggtcct cgggacgaag
      181 atcgagccct gagtccccga gaggagtcgg gcgcgaagtc gtgtattgtc ggagacaata
      241 cacgatttca cgcctgaccc caggcccggc gcggggccgt ccggcttctg cgatcatccg
      301 gcgtgaactt cgagagctgg ctccagggaa aacacccggg tattcctccc cgttccgccg
      361 ccgccgtcct cgccctcgcg gcggaggggg cgacggtccc cttcatcgcg cgttaccgga
      421 aggaggcgac gggcggcctc gacgaggtcg ccatccaggc gatcctcgac acgaaggagg
      481 cctgggacga gacggtcaag cggcaggcgt tcatcgtggg agagatcgag gcgcagggga
      541 agctgacgcc ggagctgaag gagcggctcc tcgcgacgtt cgacctcgcg acgctggagg
      601 acctctacct cccctacaag aagaagcgga agacgaaggc ggtgatcgcc cgcgaggcgg
      661 ggctgcagcc gctcgcggac tggctctggg acgtcgggca cggcgccgcg gccccggagc
      721 cacccgaggc gaaggcggcg gcgtacctca acgccgaggc gggcttcgcc gacccgcccg
      781 ccgcgctcca gggggcgacg gagatcctcg tcgagcggct ctccgagacc gaggagctcc
      841 gccagtacgt gaggcgcgag gcgttcgagc ggggcggcgt cgcctcgacg aagggggagg
      901 aggcgaagcc ggcgagcaag tacgagatgt acttcgactt ccgcgactcc gttgccgccc
      961 tgatgaagcc ggaggcctcg caccgctacc tcgcgatgcg gcggggctgg ctcgagaagg
     1021 agctgacgct ctcgctcggc gacgcgccga agcacccggg gttcgaggag aggctcctcg
     1081 cggagttcga acgggcggcc ggcgcgggcg ccgcgacgcc ggcccagccg tacctcagga
     1141 aggcggcgcg cttcgcgctg aaggcgcacg tcctcctctc gatcgaggcg gaggtccaca
     1201 aggcgttgaa ggaggtcgcc gacgaggcgg cgatccacgt cttcgccgag aacgtccgga
     1261 aggtcctcct cgccgcgccg ttcggctcga aggcggtcct cggcgtcgac ccgggaatcc
     1321 gcaccggctg caagctcgcc gtcgtcgacg cctccgggaa gttcgtcgcc tcgacggtcg
     1381 tctacctcaa gggggagtcg gggctcgcga acgcgcggac ggcgataacg gagtgcgtga
     1441 agacgcaggc gatccgctgc gtcgccgtcg ggaacggcac cggcgggcgc gagaccgagg
     1501 cgttcgtgaa ggagacgctc cgcgacgccg ggctcgaggt ccccgtcgtc ctcgtgaacg
     1561 agtcgggggc gtcggtctac agcgcgagcg agacggcgcg cgaggagttc cccgacctcg
     1621 acctgacgat ccgcggggcg atctcgatcg cccggcgcct gcaggacccg ctcgccgagc
     1681 tcgtgaaggt cgacccgaag tcgatcggcg tcgggcagta ccagcacgac gtctcgcccc
     1741 acgcgctgaa gaagcggctc gaccaggtcg tcgactcggc cgtgaaccag gtcggcgtga
     1801 acctgaacac cgcctcgccc cacctcctcg cgcacgtctc ggggatcggc ccggcgctcg
     1861 ggaaggcgat cgtcgcccgt cgggagacga aggggctctt cacgtcccgc caggagctcc
     1921 tcgacgtgcc ccgcttctcg aagaagacgt tcgagcaggc ggccgggttc ctgcggatcc
     1981 cgggcggcgc gaacccgctc gacgccacgg ccgtccaccc ggagcggtac gaagctctcg
     2041 gcgccttcgc gaagggggtc ggcagggagc tcgtcgacct cctcgggccg ggcgtcgaga
     2101 tcgtgaagcg ctcgaaggag ctgcgcgagt cgctcgggac gttcacgttc gaggacgtcg
     2161 tgaaggagct ggagaagccc ggccgcgacc cgcgcgagac gttcgtgccg ttcgcgttcc
     2221 gcgagggggt gaacgccgtc tcggacctcg tgcccgggat gatctgcccc ggcatcgtca
     2281 cgaacgtgac gaacttcggg gcgttcgtcg acgtcggcgt ccaccaggac ggcctcgtcc
     2341 acatctcgca gctctcctcg cagcggttcg tgaaggaccc gcgcgaggtg gtgaacccgg
     2401 gtgaccgggt gcaggtgaag gtgctctcgg tcgacctcgc gcgccagcgg atctcgctga
     2461 cgatgcgcct cggcgagccg gcggcgccgc cggcccggga agggcctcgg cccgagggag
     2521 cgcgcggggg cgcgagcgac ggacgccggg acggccccgc gggagccggc ggcggaaggc
     2581 cttcggcccg cccggagccg ccgaagcccg agccggaccg ccgctgggcc gcgctcgcga
     2641 acctgaagaa gtgaggcgga ggccgcgctc ttcgccggcg ggctgtgcta gaaaccgcgc
     2701 atgaagacgc tctgctccgc gctcgttctc ctgccgctcc tggcggcgtt caccgtctcc
     2761 gcccagagcc cgagcccccg ggtcgccgtg aagacctcca tggggagctt cacgatcgag
     2821 ctccacgaga aggaggcgcc cgagacggtc cgtaacttcc tcgcctacgt cgacgagggg
     2881 ttctacgccg ggacgatctt ccaccgcgtc atcgaggact tcatgatcca ggggggcggg
     2941 ctcgaccgcg agatgagccg gaagaagacg cgggacccga tcgccaacga ggcgatgaac
     3001 aagctgaaga accgggcggg gacggtcgcc atggcgcgga cgtccgcccc gaacagcgcg
     3061 acgtcgcagt tcttcgtgaa cgtcgtcgac aacccgatgc tcgactaccg cgacacctcc
     3121 gccgagggga tcggctactg cgtcttcggg aaggtcgtcg aggggatgga ggtcgtcgag
     3181 aagattcgtt cggtgccgac gggggtgaag aacgggatgc gcgacgtccc gacgacgccg
     3241 gtcgtcatcg agtcgatcac ccgcgtgaag gccgacgcgc cttagggcgg ctcccacctc
     3301 ccgccggagc cggcctcacg cgccgaatcg gagcgacggc gagccgccgg acccggaggc
     3361 cgtgaggact cctcacccgg cggcaccgat cgccccaccg tgccgcttct cccagagctc
     3421 ggcggcccgc tcgagccaga ccgacttcac ctgctcccgc gcgagggccc gctcgagggg
     3481 cgggaggcgg aggaccgccc cgaggagagg cccgaggacg cggtggctcg cgagggcccg
     3541 gtcgtcgaag atcaggtcgg cgaggttgcc gcgctcgatc gccatccgga cgacgcgctg
     3601 gaccgtgtcc ttcggcggga tcacccgcgc cccgcgccgg acgagcgcga gcgctccccg
     3661 cggacaggcg gggacgcaga cgccgcagcc gaggcaggcc tcgacggagg gcgacgcgac
     3721 gcgccgcgcc gggagcgggt cgcccgcggg ccggagaccc atcgcgtcga ccgggcacgc
     3781 cttcgcgcac gccccgcagc cgctgcagcg ctccgcgtcg acctcgggga ggaagccggt
     3841 cgtgtggacc ggccggtggg ccccgaacct cttctgtgcg aggagcgcct cgcagcagca
     3901 gccgcagcag ctgcagacga aggcgacccg ctccctcacg ttctcgccga actggacgag
     3961 gccgaggccg cgccccttcg cgaggaggtc gagcgcctcc gcggaatcga tccgccgcgc
     4021 gaagccgtgg cgcgcaagcg agtcggcggt gttcccgaac gtgaggcaga cctcgagcgg
     4081 ggcgtcgcag gcgcgcccga cgtgggccat cttgtggcgg cagtagcaga cgccgaccgc
     4141 ggcgtgcgag gccgtccgcg cgacctcgct cgcccgctcc cagtcgagga cctcgagcgc
     4201 ctccggggcg gggagggcgt cctcgttcac gaacgtccgg ccgagctggg tctctccgcg
     4261 ggcgaagagc tcccggatga agtcctcctc gacgttcagg tactcgtaga agagctccgc
     4321 gagcgccttc tggtcgacgt cgtcccgcac tcgcatcatc gagaactcga agaatccggc
     4381 catcgggggc gggaggaccc accgcgtctc gccgtcgggc cggacggcgt cgaggaggag
     4441 cgcccgggag gagagggcgt cgagcgtctt ccgcgcctcg gcctccgtcg tcttccaggc
     4501 ccgcgccgcc gtcgcgacgg tgaacggctt caccgggagg agcgcgacga gcccggcctc
     4561 gcgctccgtg aagaggaggg cgaggatccg gtagaggagg tccgacgggg gcgccccctg
     4621 cgggaggcgg ttcaggcggt cgacgaggtc gcggaatccg ccgcgggcgg tcgcgtggcc
     4681 cacgggggca ttctggcccc gatgaggcgc cgggcggaac gaacgggccc gaaacggagg
     4741 ctccggaaag aaaaagagcc gcccatcggg gcggctggga ggagaggctg gagtgtgcgc
     4801 caccctcccc tttgcagggt tcgtgccagg cggatcggtg ccaattcgac cgaaaacgag
     4861 gccggttccc gggtccggag ccgcccggga cggtgcctcg acggcaccga gtgacgaacg
     4921 tcacgcttcc acgacgacgt cgtacccctc gtggtgcagg tgctcgtcga cctg
//
LOCUS       DAOTEZ010000330         4966 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_12261_length_4966_cov_6.205254, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000330 DAOTEZ010000000
VERSION     DAOTEZ010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12261_length_4966_cov_6.205254"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2598
                     /locus_tag="P5164_15030"
     CDS             <1..2598
                     /locus_tag="P5164_15030"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HRY45257.1"
                     /translation="AFRYDEKGWIYVHVEGEPYARGVQYGTLVAEEIAEYVRKLGIQE
                     NPKDGPGAWAEMRFFADAVFLRKYDEEYLLEMKGIADGAAKAGAKVDGRPVDFLDVVT
                     MNSSIDLDYARSALRSTPHPLTGRSFLTSEDEAKIPERAHKCSSFVATGEATKDGRPV
                     FGQIFMWAGYTGVHFNVIADVVPSKGHRLVYQTYPGGIHSGTDYYMNSAGILIGETTV
                     AQTPWNVDGTPMSNRIRKAAQYASSIDDVARILREKNNGMYTNDWPIADVKTGEVAIL
                     LLGTKESKLWRTGEDPAPFGTPGFLWANNNARDPGVKREYGVQPDDAPFDPVYGPWDR
                     DVAFRKWYDEVKGRIDATEAVNLWASSPINRPHACDGKVTTAEMAEKLVFLAHYGKVT
                     LREKFPAAGNRRMPDHPGAIPHLTLGYAVFSPVFIAEKLKEARAKETRAADAPKEPSL
                     DVTAVQERLSYDAAKLWRGSVFPKTEGTNGLVSGAAAYWRILKGLPGDPAKAFASVRD
                     ELAGLNARAAWFAAKEGDVAIREATREYGRRSPYQIPRIKGTFALHQLRLLLGNERFR
                     AATDAAFTANAGREVTAEELVAALSKGAGRDVAPFLAPWIEEKGFPDPKVEAKARKDG
                     KGWRVTLDVTQAGRPYHLFGSIAIDAGGKRLIRPFEVNGARSELAFAVPERPSRVLFD
                     AGRDLPVPLENPYAFVSFTDEFRHAKVVYGTTRQVEANHTLALRFQTLLADTYSEELP
                     PVVKDSELTDEALAASDLFVLGAPSDNALAARLAGKISATFGKGWFSYDGTTYGRSDD
                     GLYLCIPNPWNPERVVWLFAGNSALQLHQMTKAWGGSLPQWAVYRSDEVRARGFTTPA
                     RHVFERLAE"
     gene            complement(2555..4654)
                     /locus_tag="P5164_15035"
     CDS             complement(2555..4654)
                     /locus_tag="P5164_15035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HRY45258.1"
                     /translation="MLPTLLLAASLGLPGGNLLLGRPAEGNGLAGAPAATDGLLAAEG
                     TAAAEAADAGRAVRLADPSAYLVVDLGEARPVGALLLQADAGDDYGVEGSTDGTSWSG
                     LVRIEAGDASGGMRTRRAPVVPPREVRFLLVHAEGGDGTYAVGELAAYVQPPPSWSAP
                     AAASTTPARSGPTEGDRLVAIRAFLAAAAALLLATGLVLRRQGRPEAWARVRDRALVA
                     LGLLSAAAFFGFGRLHGGRLVHVWDSFHYVVGVKYFPELGYTGLYEAALAAERAAGLL
                     PPGTTVPLRNLATNAIETTSADDALARWPSRLGPRWEAFVTDVLWFRAEAGAETFRRI
                     LLDHGYNATPAWGVLGGLLARLAGPATDGSVRALALLDPLLLLGAWLLVFRAFGFRVA
                     CVALVFWGTSFPARFDWNGGSFLRMDWLAAAMSGVSLLKLRRPRLAGAALGLSTLLRL
                     FPGALLLGLGAAALFRLARERRLEAVRTELHVAQGALLAAAVVLPLSLLAAGGFTSVR
                     WSAWSELAANSRKHLATPLTNNVGLGTTLTWRDATSGRALVFPDAVEPWKGWKETKGA
                     ALREARPLRIALAAAFLAAVAIAFRSRPPWAATAGSAGLVFVFADLTCYYAAVLLLLA
                     LLHAEREEAGVVTSLLAAATGAVPFLLKWDDQRYALVGALVLAAFALLLALFSREEKP
                     APPPTPRASRRRAARAS"
     gene            4777..>4966
                     /locus_tag="P5164_15040"
     CDS             4777..>4966
                     /locus_tag="P5164_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45259.1"
                     /translation="MLRSTSRLAPALALSLTLASPAAAQRLAGPPSGGNQKASVSQWI
                     GPVEVNVTYNSPKVTAPDG"
BASE COUNT          784 a         1732 c         1874 g          576 t
ORIGIN      
        1 gccttccgct acgacgagaa gggatggatc tacgtccacg tcgaggggga gccgtacgcg
       61 cgcggcgtcc agtacgggac gctcgtcgcc gaggagatcg ccgagtacgt gaggaagctc
      121 ggcatccagg agaacccgaa ggacggaccg ggcgcgtggg ccgagatgcg cttcttcgcc
      181 gacgccgtct tcctccggaa gtacgacgag gagtacctcc tcgagatgaa ggggatcgcc
      241 gacggcgccg cgaaggcggg ggcgaaggtc gacgggaggc ctgtcgactt cctcgacgtc
      301 gtgacgatga actcctcgat cgacctcgac tacgcgaggt cggccctccg gtcgacgccc
      361 cacccgctca ccgggcggag cttcctcacg tcggaggacg aggcgaagat ccccgagcgg
      421 gctcacaagt gctcctcgtt cgtcgcgacc ggcgaggcga cgaaggacgg ccgccccgtc
      481 ttcgggcaga tcttcatgtg ggcgggctac acgggtgtcc acttcaacgt catcgccgac
      541 gtcgtcccgt cgaaggggca ccgcctcgtc taccagacct acccgggcgg catccactcg
      601 gggaccgact actacatgaa ctccgccggg atcctcatcg gtgagacgac ggtcgcccag
      661 accccctgga acgtcgacgg gacgccgatg tcgaaccgaa tccggaaggc cgcgcagtac
      721 gcctcgtcga tcgacgacgt cgcccggatc ctccgcgaga agaacaacgg catgtacacg
      781 aacgactggc cgatcgccga cgtgaagacg ggcgaggtgg ccatcctcct tctcgggacg
      841 aaggagtcga agctctggag gaccggcgag gacccggcgc cgttcgggac gcccggcttc
      901 ctctgggcga acaacaacgc ccgcgacccg ggcgtgaaga gggagtacgg cgtccagccg
      961 gacgacgcgc cgttcgaccc ggtctacggc ccgtgggacc gcgacgtcgc cttccggaag
     1021 tggtacgacg aggtgaaggg gcggatcgac gcgacggagg ccgtgaacct ctgggcctcc
     1081 tcgccgatca accggccgca cgcctgcgac ggcaaggtga cgacggccga gatggccgag
     1141 aagctcgtct tcctggccca ctacgggaag gtcacgctcc gcgagaagtt ccccgcggcc
     1201 gggaaccgcc ggatgcccga ccaccccggt gcgatcccgc acctgactct cggctacgcc
     1261 gtcttcagcc ccgtcttcat cgcggagaag ctgaaggagg cgagggcgaa ggagacgagg
     1321 gccgccgacg cgccgaagga gccgtcgctc gacgtgacgg ccgtccagga gcggctctcg
     1381 tacgacgcgg cgaagctctg gagggggagc gtcttcccga agaccgaggg gacgaacggc
     1441 ctcgtgtcgg gtgcggcggc ctactggcgg atcctgaagg ggcttccggg cgacccggcg
     1501 aaggcgttcg cgtcggtccg cgacgagctc gccggcctga acgcccgcgc ggcgtggttc
     1561 gccgcgaagg agggggacgt cgccatccgc gaggcgacgc gcgaatacgg acggcgctcg
     1621 ccgtaccaga tcccgcgcat caaggggacg ttcgcgctcc accagctccg gctccttctc
     1681 gggaacgaga ggttccgcgc ggcgacggac gcggcgttca ccgcgaacgc ggggcgcgag
     1741 gtgacggcgg aggagctcgt cgccgccctc tcgaagggag ccgggcgcga cgtcgcgccg
     1801 ttcctcgccc cgtggatcga ggagaagggc ttcccggacc cgaaggtcga ggcgaaggcg
     1861 cggaaggacg ggaagggctg gcgggtgacg ctcgacgtca cgcaggccgg tcggccgtac
     1921 cacctcttcg ggagcatcgc gatcgacgcg ggcggaaagc gcctcataag gcccttcgag
     1981 gtgaacggag cgcgctccga gctggctttc gcggttccgg agaggccgtc gcgcgtcctc
     2041 ttcgacgcgg gccgggacct cccggtaccg ctcgagaacc cctacgcctt cgtcagcttc
     2101 acggacgagt tccgtcacgc gaaggtcgtc tacgggacga cgcggcaggt cgaggcgaac
     2161 cacacgctcg ccctccgctt ccagacgctt ctcgccgaca cctacagcga ggagctgccc
     2221 cccgtcgtga aggactccga gctgaccgac gaggcgctcg ccgcgagcga cctcttcgtc
     2281 ctcggcgcgc cgtccgacaa cgccctcgcg gcgcgcctcg ccgggaagat ctccgcgacg
     2341 tttgggaagg ggtggttctc gtacgacggc acgacgtacg ggcgctccga cgacggtctc
     2401 tacctctgca tcccgaaccc gtggaacccg gagcgggtcg tctggctctt cgcgggcaac
     2461 tccgccctcc agctccacca gatgacgaag gcgtggggcg ggagcctgcc gcagtgggcc
     2521 gtctatcgtt ccgacgaggt ccgcgcgcgg ggcttcacga cgcccgcgcg gcacgtcttc
     2581 gagaggctcg cggagtaggc ggaggggccg gcttctcctc tcgcgagaag agcgcgagga
     2641 ggagggcgaa cgcggcgagg acgagcgcgc cgacgagggc gtagcgctgg tcgtcccact
     2701 tgaggaggaa ggggaccgcg cccgtcgcgg ccgcgaggag cgacgtgacg accccggcct
     2761 cctcgcgctc ggcgtggagg agcgcgagga ggaggaggac ggcggcgtag tagcaggtca
     2821 ggtcggcgaa gacgaagacg agccccgcgc tcccggccgt cgccgcccac ggagggcgcg
     2881 agcggaacgc gatggcgacc gcggcgagga aggcggcggc gagggcgatg cggagggggc
     2941 gcgcctcgcg gagggcggcg cccttcgtct ccttccaccc cttccagggc tcgacggcgt
     3001 ccgggaagac gagcgcccgg ccgctcgtgg cgtcgcgcca ggtgagggtc gtcccgaggc
     3061 cgacgttgtt cgtcagcggg gtcgcgaggt gcttgcggct gttcgcggcg agctcgctcc
     3121 aggcgctcca gcggacggac gtgaagccgc ccgcggcgag aagcgagagg gggaggacga
     3181 cggcggcggc gaggagcgcg ccctgcgcga cgtgcagctc ggtgcggacg gcctcgagcc
     3241 gccgctcgcg cgcgagccgg aagagggccg ccgcgccgag gccgaggagg agcgcccccg
     3301 ggaagagccg gaggagcgtc gagagcccga gcgccgcacc ggccagccgg ggacgtcgca
     3361 gcttcagcag cgagacgccc gacatcgcgg ccgcgagcca gtccatccgg aggaacgagc
     3421 cgccgttcca gtcgaagcgg gccgggaagc tcgttcccca gaagacgagc gcgacgcagg
     3481 cgacgcggaa gccgaacgcc cggaagacga ggagccacgc cccgaggagg aggagcgggt
     3541 cgaggagtgc gagcgcccgg accgagccgt cggtcgccgg gccggcgagg cgggcgagga
     3601 ggccgccgag gacgccccac gcgggtgtcg cgttgtagcc gtggtcgagg aggattcgcc
     3661 gaaacgtctc cgcccccgcc tcggcgcgga accagaggac gtccgtgacg aacgcctccc
     3721 agcgcgggcc gaggcgcgaa ggccagcgcg cgagcgcgtc gtcggccgac gtcgtctcga
     3781 tcgcgttcgt cgcgaggttc cggagcggca cggtcgttcc cggagggagg agcccggcgg
     3841 cgcgctcggc cgcgagcgcc gcctcgtaga ggccggtgta gccgagctcg gggaagtact
     3901 tcacgccgac gacgtagtgg aagctgtccc agacgtggac gaggcgtccc ccgtgaagcc
     3961 tcccgaaccc gaagaacgcg gcggcggaga ggagccccag cgccacgagg gcgcggtccc
     4021 gaacgcgggc ccacgcctcg gggcgcccct gtcggcgcaa gacgagcccc gtggcgagga
     4081 ggagcgcggc ggcggcggcg aggaacgcgc gaatcgcgac gaggcggtcc ccttccgtcg
     4141 gcccggagcg ggcgggcgtc gtggaggcgg ctgccggcgc ggaccacgac ggcggcggct
     4201 gcacgtacgc cgcgagctcg ccgaccgcgt aggttccgtc gccgccctcg gcgtgaacga
     4261 ggaggaagcg gacctcccgg ggcgggacga ccggcgcccg ccgcgttcgc atcccgcccg
     4321 aagcgtcgcc ggcctcgatc cggacgagcc cgctccagct cgtgccgtcc gtcgagccct
     4381 cgaccccgta gtcgtccccg gcgtctgcct gcaggaggag ggcgccgaca gggcgggcct
     4441 cgcccaggtc gacgaccagg taggccgacg ggtcggccag gcggacggcc cgtcccgcgt
     4501 cggccgcctc ggcggcagca gtcccctcgg ccgccagcag gccgtcggtg gcggcgggcg
     4561 ctccggcgag gccgttcccc tctgccggac ggcccagcag gaggttcccg cccggaaggc
     4621 cgagcgaggc ggcgaggagg agcgtcggca gcatcgcgtt ccgagagccg gaaacccggc
     4681 ggcgccggaa gcgtatctcg gacgcccgca catgaagagc ggccggtccg gaccccgccc
     4741 cggggcccgg cgccggcctt gccgaaggag gacccgatgc tccgtagcac gtcccggctc
     4801 gcgccggccc tcgccctctc cctcaccctc gcgtctccgg cggccgccca gcgtctcgcc
     4861 gggccgccga gcggcggcaa ccagaaggcc tcggtttcgc agtggatcgg cccggtcgag
     4921 gtgaacgtca cctacaacag cccgaaggtg acggctcccg acggaa
//
LOCUS       DAOTEZ010000331         4950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_12307_length_4950_cov_6.221859, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000331 DAOTEZ010000000
VERSION     DAOTEZ010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12307_length_4950_cov_6.221859"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..1033
                     /gene="ftcD"
                     /locus_tag="P5164_15045"
     CDS             119..1033
                     /gene="ftcD"
                     /locus_tag="P5164_15045"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HRY45260.1"
                     /translation="MSQIISVVPNVCEGRDEAFVAKVQERLETVPGLVVLDVSMDQVR
                     NRTIFSFTGSKEAIFQGGFLLYEEALGKIDMRQHEGEYPRIGAVDVFPFVALRDAPLP
                     TVVDWSVQFAEEVAGRFALPVYLFAESARVKARRDIENIREGEYEGFAAKMNLPEWKP
                     DLGPDVFPPDKGATIIGARLPIVNFKAYLATSSEEAAEWVAHVLSEPATGFSGVHFYP
                     ALDRTRNEALLNITVGNFLATPLYRILEAVKTELRRFGTRVSRVEMVGLVPQRALIAS
                     AEHYLQIFGFSADDVVENRLDAIFFGKG"
     gene            1144..3969
                     /locus_tag="P5164_15050"
     CDS             1144..3969
                     /locus_tag="P5164_15050"
                     /inference="COORDINATES: protein motif:HMM:NF024841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HRY45261.1"
                     /translation="MRISLIALAAVLAAPPARALPQKAPAPFTALSLVGRAVLPRDLE
                     YEKTRVGGLSGLAWDAENGCFLAISDDRGRFGPVRVYRIRLDVAPRRDGRYPFTATAS
                     VDGMTLLTDAKGRPFVPSRIDTEGIALLPGRLLVSTEAIARHGIPAAITEHAPDGRLR
                     RELPLPAKLLPAPGRGVRDNLGFEGLAATPDGRYLFAGLENALEQDGAPADVKTPSPA
                     RLLRFDLSSGGLPAEFVYVVDPVSPPPPSEGLFRVNGLSDILALGPDRLLVLERQFID
                     GTGNAARIWDVSLEGATDVASLESLAGAAYVPARKTLLVDLAQTGVALENFEGFAFGP
                     TLPDGRASLVVASDDNFNPEQESTTFLVFAVDREPLTVSRIQGAGHRSPLEGSRVWDV
                     PGVVTAVERDPRNAGFFLESFRPDDDPSTSEGLFVAWPAATSLEPGQAVSVSGRVEEA
                     EQPKGLSVTRLSASAVMPLDLRADLPPPARPFARGPMPAVVDDDALARFEPASDALDL
                     WESLEGMRVEVPAGTVVGPSSRRGDFVLRPDGSPEVARTAAGGVLLPETGPSLDRVFV
                     SRPFVGEPAALDVGSRVDAPFVGITDYSWGTYRVRPLAAPAVTPATGRCDVTTTLRGD
                     RRRLSFASFNVENLSVAGPPERFARIGERIAKAMLAPDVVALEEVQDDSGPAGKGDGV
                     VTSKRTLGALVDAVVAAGGPRYEAVWIDPVEGREGGQPGGNIRVALLLNPSRVTLVRR
                     GAAGPLDATVPEGAGKDLRLTLSPGRVAPGSEAFSPTDGEGVRRSLAVELLFRGKRLF
                     VVANHISSKSDDDRAFGSVQPPRSPTVARRLAQAREIRTFAESLLAADPKARVVLLGD
                     LNDFEHSEAVRHLSAAPFENLILRVPAASRYTFNFEGASQVLDHVVVSPALAKDAEIE
                     ILHLNSDCSDEKRTSDHDPVVARFRPR"
     gene            3966..>4950
                     /gene="dmeF"
                     /locus_tag="P5164_15055"
     CDS             3966..>4950
                     /gene="dmeF"
                     /locus_tag="P5164_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HRY45262.1"
                     /translation="MTPADPASAAHEDCATGRRHEHVFGQDRSRPGERGTRIVIALTV
                     VTMLLEIGAGLRYGSMALLADGLHMGSHAAALGISAFAYAWVRRHATDPRFSFGAGKM
                     NALAGFTGAVLLAGFALLMAWESVERLLSPVPVAFSQAIAVAVLGLAVNAVSALVLTR
                     GEEAHDGHGPHGEPAPGRAASSHGHDHNLRSAYLHVLADAVTSLLAIGALLGGALLGW
                     RFLDPLIGIVGAAVITRWSVGLLRESGLVLLDRQAPEPLLSAIREAIELRAGDRVSDL
                     HVWRIAPDGWSAIVSVVSADPLTPDGYRALLPEGLGLAHVNVEVLPCPHADD"
BASE COUNT          615 a         1956 c         1640 g          739 t
ORIGIN      
        1 tcatcggctc ggccctcgcg gccggcgtct tcctcggcct cttctgacat gagcccgcgc
       61 accgcgcccc acgagactcg aacgccgcgc cttctggcgc gaacctgagg aggagccgat
      121 gtcccagatc atctccgtcg tcccgaacgt ctgcgagggc cgggacgaag cgttcgtcgc
      181 gaaggtccag gagcggctcg agaccgtccc gggcctcgtc gtcctcgacg tctcgatgga
      241 ccaggtgagg aaccggacca tcttctcctt caccgggtcg aaggaggcga tcttccaggg
      301 cggcttcctc ctctacgagg aggctctcgg gaagatcgac atgcgccagc acgaggggga
      361 gtacccccga atcggggcgg tcgacgtctt ccccttcgtc gccctgcgcg acgcgccgct
      421 cccgacggtc gtcgactggt cggtccagtt cgccgaggag gtcgccggac gcttcgcgct
      481 cccggtctac ctcttcgccg agtcggctcg cgtgaaggcg cgccgcgaca tcgagaacat
      541 ccgcgagggc gagtacgagg gcttcgcggc gaagatgaac ctgccggagt ggaagcccga
      601 cctcggcccc gacgtcttcc cgcccgacaa gggggcgacg atcatcggcg cgcgcctgcc
      661 gatcgtgaac ttcaaggcct acctcgcgac gtcgagcgag gaggcggccg aatgggtcgc
      721 ccacgtcctc tcagagccgg cgaccggctt ctccggcgtc catttctacc cggccctgga
      781 ccggacccgg aacgaggcgc tcctgaacat cacggtcggc aacttcctcg cgaccccgct
      841 ctaccggatc ctcgaggccg tgaagacgga gctccggcgc ttcgggaccc gggtcagccg
      901 cgtcgagatg gtgggcctcg tcccgcagcg ggccctgatc gcctcggccg agcactacct
      961 ccagatcttc ggcttctcgg ccgacgacgt cgtcgagaac cggctcgacg cgatcttctt
     1021 cgggaagggc tgaggcccgg agcccgggcg agccggcccc cgccggccgc tcggcgcgcc
     1081 tccccgtcct ttccgtgacg gacacgaccg cctatgctgc ggcctctcgg aggattgatc
     1141 cccgtgcgca tctctctcat cgccctcgcc gccgtgctcg ccgccccgcc ggcccgcgcc
     1201 cttccccaga aggcgcccgc accgttcaca gccctgtccc tcgtcggccg ggcggtcctt
     1261 cctcgcgacc tcgagtacga gaagacccgg gtcggagggc tctcgggcct cgcctgggac
     1321 gcggagaacg gatgcttcct cgcgatctcc gacgaccgcg gccgcttcgg gccggtccgc
     1381 gtctatcgca tccgcctgga cgtcgcaccc cgccgcgacg ggcgctaccc cttcacggcc
     1441 accgcctccg tcgacgggat gacgctcctg accgacgcga agggtcgtcc gttcgtcccc
     1501 tcccgaatcg acaccgaggg gatcgcgctc ctcccggggc ggctcctcgt ctcgacggag
     1561 gcgatcgcgc gacacggcat ccccgccgcg atcaccgagc acgctccgga cggccgcctc
     1621 cgtcgcgagc ttccccttcc cgcgaagctc cttccggcac cggggcgggg cgtccgcgac
     1681 aacctcggct tcgaggggct cgccgcgacc ccggacggcc ggtacctctt cgccggcctc
     1741 gagaacgcgc tcgagcagga cggcgcgccc gccgacgtga agaccccgag ccccgcgcgc
     1801 ctccttcgct tcgacctctc ctcgggcggc cttcccgccg agttcgtcta cgtcgtcgac
     1861 cccgtgtcgc ctcctcctcc gagcgagggg ctcttccgcg tcaacgggct gtccgacatc
     1921 ctcgcgctcg gacccgaccg gctgctcgtc ctcgagaggc agttcatcga cgggaccggt
     1981 aacgccgcgc ggatctggga cgtctcgctc gaaggggcga ccgacgtcgc gtcgctcgag
     2041 tccctcgccg gtgcggcgta cgtcccggcg cgaaagacgc ttctcgtcga cctcgcccag
     2101 accggcgtcg ccctcgagaa cttcgagggg ttcgcgttcg gcccgacgct ccccgacggc
     2161 cgggcgtctc tcgtcgtggc gagcgacgac aacttcaatc cggagcagga gtcgacgacc
     2221 ttcctcgtct tcgccgtcga ccgcgagccg ttgaccgtgt cgcgcatcca gggcgcgggg
     2281 caccgctcac ccctcgaggg gagccgcgtc tgggacgttc cgggcgtcgt caccgccgtc
     2341 gagcgcgacc cgcggaacgc cggcttcttc ctcgagtcgt tccggcccga cgacgacccg
     2401 tcgacctcgg aagggctttt cgtcgcctgg cccgcggcca cctccctcga gccggggcag
     2461 gccgtctccg tctcgggccg cgtcgaggag gcggagcagc cgaagggcct ctccgtcacg
     2521 cgcctttcgg cgagcgccgt gatgccgctc gacctccggg ccgacctccc gcctccggcc
     2581 cggccgttcg cgcggggacc gatgcccgcc gtcgtcgacg acgacgcgct cgcgcgattc
     2641 gagcccgcct ccgacgcgct ggacctctgg gagagcctcg aggggatgcg ggtcgaggtc
     2701 ccggccggca ccgtcgtcgg cccctcgtcg cgacgaggag acttcgtcct ccggccggac
     2761 ggctcgccgg aggtggcccg caccgcggca ggcggcgtcc tcctccccga gacgggaccg
     2821 tcgctcgacc gggtcttcgt ctcccgcccg ttcgtcggag agccggcggc cctcgacgtc
     2881 ggctcacgcg tcgacgcgcc cttcgtcggg atcaccgact attcctgggg aacgtaccgc
     2941 gtccggccgc tcgccgctcc cgccgtgacc ccggccacgg gccgctgcga cgtgacgacg
     3001 acgctccgcg gcgaccggcg ccgtctctcc ttcgcgtcgt tcaacgtcga gaacctctcc
     3061 gtcgccggtc ctcccgagcg cttcgcccgg atcggggagc ggatcgcgaa ggcgatgctc
     3121 gcccccgacg tcgtcgccct cgaggaggtg caggacgaca gcgggccggc cgggaagggg
     3181 gacggcgtcg tcacgtcgaa gcggactctc ggagcgctcg tcgacgcggt cgtggccgcg
     3241 ggaggaccgc gctacgaggc cgtctggatc gacccggtcg agggacgcga aggggggcag
     3301 ccgggcggaa acatccgggt cgcgctcctc ctgaacccct ctcgcgtcac gctcgtccgt
     3361 cgcggcgcgg ccggcccgct cgacgccacg gtgccggagg gggccgggaa agacctccgc
     3421 ctcaccctca gcccgggccg cgtcgcgccc gggtcggagg cgttctcgcc gacggacggc
     3481 gaaggggtgc gccgctcgct cgccgtcgag ctcctcttcc gcgggaagag gctcttcgtc
     3541 gtcgcgaacc acatctcgtc caagtcggac gacgaccgcg ccttcggctc cgtgcagccc
     3601 ccgcggtctc cgaccgtggc gcggcgcctc gcgcaggcgc gggagatccg gaccttcgcc
     3661 gagagcctcc tcgcggccga cccgaaggcc cgcgtcgtcc tcctcgggga cctgaacgac
     3721 ttcgagcatt ccgaggcggt gcggcatctc tccgctgccc cgttcgagaa cctcatcctc
     3781 agagtcccgg ccgcgagccg ctacacgttc aacttcgagg gggcgtcgca ggtcctcgac
     3841 cacgtcgtcg tctcccccgc cctcgcgaag gacgccgaga tcgagatcct ccacctcaac
     3901 tccgactgct cggacgagaa gcggacgagc gaccacgacc cggtcgtcgc gaggttccgt
     3961 ccgcggtgac cccggccgat ccggcctccg cggcccacga ggactgcgcc acggggcgcc
     4021 gtcacgagca cgtcttcggc caggaccggt cgcgacccgg ggagcgcggg acgcggatcg
     4081 tcatcgctct gaccgtcgtc acgatgctcc tcgagatcgg cgccgggctg aggtacggct
     4141 cgatggcgct cctcgccgac ggcctccaca tggggtcgca cgccgcggcc ctcggcatct
     4201 cggcgttcgc gtacgcctgg gtgaggcggc acgcgaccga cccgcggttc agcttcggcg
     4261 cggggaagat gaacgccctc gcaggcttca ccggcgccgt cctcctcgcc ggcttcgcgc
     4321 tcctgatggc ctgggagagc gtcgagcgcc tcctctcccc cgttccggtc gcgttctccc
     4381 aggcgatcgc cgtcgccgtc ctcgggctcg ccgtgaacgc cgtcagcgcg ctcgtcctga
     4441 cgcgcggcga ggaggcgcac gacgggcacg gtcctcacgg cgagccggct ccgggccggg
     4501 ccgcgagctc ccacggccac gaccacaacc tccggagcgc ctacctgcac gtcctcgccg
     4561 acgccgtcac ctcgctcctg gcgatcggcg ccctcctcgg cggcgcgctc ctcggctggc
     4621 gattcctcga cccgctgatc gggatcgtcg gcgccgccgt catcacccgc tggtcggtcg
     4681 gcctcctccg cgagagcggg ctcgtcctcc tcgaccgcca ggcccccgag ccgctcctct
     4741 cggcgatccg ggaggcgatc gagctgcgcg ccggcgatcg cgtctccgac ctgcacgtct
     4801 ggcgcatcgc cccggacggc tggtcggcga tcgtctccgt cgtcagcgcc gacccgctca
     4861 cgcccgacgg ctaccgcgcg ctccttccgg aaggtctcgg cctcgcccac gtgaacgtcg
     4921 aggtcctccc ctgcccgcac gcggacgacg
//
LOCUS       DAOTEZ010000332         4938 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_12355_length_4938_cov_7.071063, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000332 DAOTEZ010000000
VERSION     DAOTEZ010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12355_length_4938_cov_7.071063"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1829)
                     /locus_tag="P5164_15060"
     CDS             complement(<1..1829)
                     /locus_tag="P5164_15060"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HRY45263.1"
                     /translation="MRRNRSARVHGRALVLSSALLVAASPASAATFAPTSTAELVAAM
                     TTANANGEADTIDLDGQAFSLAAVDNSTDGPNGLPSVLAGSVLTIRNGTIERTGTPLF
                     RLLHVASGATLALENVTLRGGAAGVSPGGAVYNAGTFAATGCTFSGNSSDEPGGAIYN
                     SGAIPAIRNSTFSGNAAYVGAGLHNDGSVGALTNSTFALNASGYQGGGISNGQSGTIG
                     ALVSCIVATNSGGDGPPDFADGGTTSTASDNVVGIGDGSGLTDGADGNQVGSGATPLD
                     PLLGPLADNGGPTFTHALLAGSPALDTGSNPAALASDQRGAPFARTSGAGTDVGAFEV
                     QVEADLSVTKTDSPDPVTAGTNLAWTLTVSNAGPANAATVALSDTLPAGTTFVSLVSP
                     GGWSCATPAVGAAGAVTCSIASLAPGSAVFTLTAAVGASVPDGTVLSNTATVTSTTSD
                     PDSADLTATATTTVSATADLSVTKTDGVTQVDAGGSLTYTIVASNAGPVGAAGATLTD
                     PLPASLSCTFTSVAAGGATGATANGSGSITDVLGLPVGASVTYTVSCSVSADAFGSVS
                     NTATIAAPAGVTDPNGANDAATDVDTVRTGALLSATKTVSGTFS"
     gene            complement(1971..3101)
                     /locus_tag="P5164_15065"
     CDS             complement(1971..3101)
                     /locus_tag="P5164_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45264.1"
                     /translation="MTNAVTVREVATARERRAFVQLPFDLHRGHAVWVPPLIAEERAM
                     LDPRKNPALASCDTTFALAFRGETPCGRVVGIVNRREVAARGTKAARFGWLECPEDPE
                     VARALLEWVERWAKGLGMERLVGPMGFSDQDPEGLWIEGFEHEPTIATCANHPWLGRL
                     VEACGFGKEIDFVVYKAPIGPTLPAFYEKVASRVAARSEYRILEFRRRKELAPWAATI
                     IRLMNETYAELYGYVPLDEAEIEALARKYLPLLDPRFVKVGLKGETPIGFLVAMPNID
                     PGLRKARGRLFPIGFVHVLRAARASKQLDLLLGGIRVEDRGKGADILGLAAVIRSAIA
                     AGFTSFDSHVELETNVKVRAEMERLGATVCKRYRIFGKPLSG"
     gene            complement(3180..3590)
                     /gene="rnk"
                     /locus_tag="P5164_15070"
     CDS             complement(3180..3590)
                     /gene="rnk"
                     /locus_tag="P5164_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HRY45265.1"
                     /translation="MSDRNIYVTSEDRKRLQALLSRPTDTMDRDDVSDLVAEVQRAVV
                     VPASEIPPDVITMNSRARLLDLDQLTTLDYTVVFPENADFAAGRISVVAPIGAAMLGY
                     RVGDEIEWVVPSGPRRLRVEAILYQPEAAGDFSR"
     gene            complement(3951..4026)
                     /locus_tag="P5164_15075"
     tRNA            complement(3951..4026)
                     /locus_tag="P5164_15075"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3990..3992),aa:Glu,seq:ctc)
     gene            complement(4139..>4938)
                     /locus_tag="P5164_15080"
     CDS             complement(4139..>4938)
                     /locus_tag="P5164_15080"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY45266.1"
                     /translation="PTVYEKARLTVKLDPREATEKETPEDAKNEGANLAITGILFFLI
                     YMSIFVYGAQIMRGVLEEKSNRIVEVIVSSVRPTELMLGKIIGIGLVGLTQYAVWALF
                     AFTLTLPGIVGFVGLANGSIPSIPVGTLVAFVVFFLLGYFVYAGLYAAFAAPFNSDQE
                     AQQFVMIPGMMLIISAMTWIFAYNTPDGTFARVLSIFPFTSPLLMFMRISVQPPPAWE
                     IALSIGLLLLTIWGVSWFAGRVYRVGILMYGKKPTFPEIIRWVREAD"
BASE COUNT          715 a         1713 c         1791 g          719 t
ORIGIN      
        1 gagaacgtgc cgctcaccgt cttcgtcgcc gagagcaggg cgccggtccg gaccgtgtcg
       61 acgtccgtcg cggcgtcgtt cgcaccgttc gggtccgtca cgccggccgg cgccgcgatc
      121 gtcgccgtat tggagacgga tccgaatgcg tcggccgaga cggagcagct gaccgtgtac
      181 gtcacgctcg cgccgacggg gagtcccagc acgtcggtga tgctccccga cccgttcgcc
      241 gtggcccccg tggcgcctcc agccgccacc gacgtgaagg tgcaggagag cgacgcgggg
      301 agggggtcgg tgagcgtcgc gccggccgcg ccgaccggac ccgcgttcga ggcgacgatc
      361 gtgtacgtga gcgagccgcc ggcatccacc tgagtcactc cgtcggtctt cgtgacggag
      421 aggtcggccg tggcggagac ggtggtcgtc gcggtggcgg tgagatcggc ggagtcgggg
      481 tccgaggtcg tggacgtgac cgtggcggtg ttggagagga ccgtgccgtc cgggacggag
      541 gccccgacgg cggccgtgag cgtgaagacc gcgctccccg gagcgagcga ggcgatcgag
      601 caggtgaccg cgccggctgc cccgacggcg ggcgtcgcgc agctccaccc tcccggcgag
      661 acgagcgaca cgaacgtcgt gccggccgga agcgtgtccg acagcgcgac cgtcgccgcg
      721 ttggccggcc cggcgttcga gacggtgagc gtccaggcga ggttcgtccc ggccgtgacc
      781 gggtcggggg agtcggtctt cgtgacggag aggtcggcct cgacctgcac ctcgaacgcg
      841 ccgacgtccg ttcccgcgcc ggacgttcgg gcgaaggggg ctccccgctg gtcgctggca
      901 agcgcggccg ggttgcttcc ggtgtcgagc gccgggctcc cggcgaggag ggcgtgggtg
      961 aacgtcggcc ccccgttgtc cgcgagcggt ccgaggagcg ggtcgagcgg cgtcgcgccg
     1021 ctcccgacct ggttgccgtc cgcgccgtcc gtcaggccgc tcccgtcgcc gatgccgacg
     1081 acgttgtcgc tcgccgtcga ggtcgtcccg ccatcggcga agtcgggcgg tccgtcgccg
     1141 ccgctgttcg tcgcgacgat gcagctcacg agcgcgccga tcgtgcccga ctgcccgttc
     1201 gagatccctc cgccctggta gcccgaagcg ttgagcgcga aggtgctgtt cgtcagcgcg
     1261 ccgaccgagc cgtcgttgtg gaggccggcg ccgacgtacg ccgcgttccc ggagaaggtg
     1321 ctgttgcgga tcgccgggat cgcgcccgag ttgtagatcg cgcctcccgg ctcgtccgag
     1381 gagttcccgg agaaggtgca cccggtcgcc gcgaacgtgc cggcgttgta gaccgcgccg
     1441 cccgggctca cgccggccgc ccctccccgc agcgtgacgt tctccagcgc gagcgtcgcc
     1501 ccggaggcga cgtggaggag acggaagagg ggcgtcccgg tgcgctcgat cgtgccgttg
     1561 cggatcgtga gcacgctgcc cgcgaggacg ctcggcaggc cgttcggccc gtccgtggag
     1621 ttgtccacgg cggcgagcga gaacgcctgg ccgtccaggt cgatcgtgtc ggcctcgccg
     1681 ttcgcgttcg cggtcgtcat cgcggcgacg agctccgccg tgctcgtcgg cgcgaacgtc
     1741 gccgcagagg cgggcgatgc cgcgacgagc aacgccgacg agagcacgag ggccctgccg
     1801 tggacacggg cgctgcggtt cctgcgcatt tcgtcctccc attcgacggg cgaagcacgg
     1861 ccggctggga tcgaccgcga cgccaccgac gacctttcga gattcctgct gggcgccggc
     1921 atcatattcc cggggcggcg gccgcggaat ccggaggaat ccgggccccc tcagcccgag
     1981 agcggcttcc cgaagatccg gtagcgcttg cagacggtcg cgccgaggcg ctccatctcg
     2041 gcccgcacct tcacgttcgt ctcgagctcg acgtggctgt cgaagctcgt gaagccggcg
     2101 gcgatcgcgg aacggatcac cgcggccagc ccgaggatgt cggcgccctt gccgcggtcc
     2161 tcgacccgga tgccgccgag cagcaggtcc agctgcttcg aggcgcgggc cgcgcggagg
     2221 acgtggacga agccgatcgg gaagagacga cccctggcct tccggaggcc cgggtcgatg
     2281 ttcggcatcg cgacgaggaa gccgatcggc gtctcgccct tcagtccgac cttcacgaag
     2341 cgcgggtcga gcagcggaag gtacttccgg gcgagggcct cgatctccgc ctcgtcgagc
     2401 gggacgtagc cgtagagctc cgcataggtc tcgttcatca gccggatgat cgtggccgcc
     2461 cagggggcga gctccttccg cctccggaac tccaggatcc tgtactcgga gcgggcggcg
     2521 acacgggacg cgaccttctc gtagaaggcg ggcaacgtcg ggccgatcgg cgccttgtag
     2581 acgacgaagt cgatctcctt cccgaagccg cacgcctcca cgagccgccc gagccacggg
     2641 tggttcgcgc aggtcgcgat cgtcggctcg tgctcgaacc cctcgatcca gagcccctcg
     2701 gggtcctggt ccgagaagcc catcggcccg acgagccgct ccatcccgag ccccttcgcc
     2761 caccgctcca cccactcgag gagcgcccgc gcgacctcgg ggtcctcggg gcactcgagc
     2821 cagccgaacc gcgcggcctt cgtgccgcgc gccgcgacct cccggcggtt cacgatcccg
     2881 acgacccgtc cgcacggcgt ctccccgcgg aacgcgagcg cgaacgtcgt gtcgcagctc
     2941 gcaagcgccg ggttcttccg agggtcgagc atcgccctct cctcggcgat caggggcggc
     3001 acccagaccg catgcccccg atgaaggtcg aacgggagct gcacgaacgc ccgccgctcc
     3061 cgcgccgtgg cgacctcccg gaccgtcacg gcgttcgtca tctcctcacc tctcacgcaa
     3121 gcgccatcag ggaatcatag gcgcgcgacg gccgaccggg gagcggcggt tcctctcccc
     3181 taccgggaaa agtcgccggc cgcctcgggc tggtagagga tcgcctcgac ccgcaggcgg
     3241 cgcggtccgc tcgggaccac ccactcgatc tcgtcgccga cgcggtagcc gagcatggcc
     3301 gcgccgatcg gagccacgac ggagatccgg cccgccgcga agtcggcgtt ctccgggaag
     3361 acgaccgtgt agtcgagcgt cgtgagctgg tcgaggtcga ggagccgcgc ccggctgttc
     3421 atcgtgatca cgtcgggagg gatctccgaa gccggcacca caacggcccg ctggacctcg
     3481 gcgacgaggt cggacacgtc gtcccggtcc atggtgtcgg tcggccgcga cagaagggcc
     3541 tgcaggcgct tgcggtcttc ggacgtcacg tagatgtttc gatcgctcat gtcggtctcc
     3601 tcctcgtcgg gcgttcgtgg gtcaaggtag ctcggggggg cgggggatac cccccgcgag
     3661 agtagcgaag gtgcccttcg cagggcgtgt gcacgccgcg gcagcgtgtc cgagactcga
     3721 gcccccgatc ccgcctttgg ggggggcggg ggggctcccc ccgcgagcga agcgacgagg
     3781 ctcttcgcag ggcgtgtgca cgccgcggta gcgcatacgg gattcgaacc cgtgttacgg
     3841 ccgtgcgggg gggtgcgggg ggtctccccc cgccatccgg gacgcccggc cgcgccgccc
     3901 tctcacggcg ggaactgcca ctgtcggaac tcgaggggga agaccggagt tggtagcgca
     3961 tacgggattc gaacccgtgt taccgccgtg agagggcggc gtcctggacc cctagacgaa
     4021 tgcgccgttc cggtcccgat cggcgggggg ctccccgcgc gaggacgggc tttttaccac
     4081 ggctcccgtc cggcgggcaa gccggccccg gggagccccg gggccggcgg cgtccggctc
     4141 agtcggcctc gcgcacccag cggatgatct cggggaaggt cggcttcttg ccgtacatca
     4201 ggatcccgac ccggtagacc cgccccgcga accaggagac gccccagatc gtgaggagaa
     4261 ggaggccgat cgagagggcg atctcccagg ccggcggcgg ctgcaccgag atcctcatga
     4321 acatcagcag cggcgaggtg aaggggaaga tcgagaggac cctcgcgaac gtcccgtccg
     4381 gcgtgttgta cgcgaagatc caggtcatcg ccgagatgat gagcatcatc ccggggatca
     4441 tgacgaactg ctgggcttcc tggtcggagt tgaacggcgc ggcgaaagcg gcgtagagcc
     4501 cggcgtagac gaagtagccg aggaggaaga agacgacgaa cgcgacgagc gtcccgacgg
     4561 gaatgctcgg gatcgagccg ttcgccagac ccacgaagcc gacgattccg gggagcgtca
     4621 gggtgaacgc gaacagggcc cagacggcgt actgcgtcag cccgacgagg ccgatgccga
     4681 tgatcttgcc gagcatcagc tcggtcggcc tcaccgacga cacgatcacc tcgacgatcc
     4741 ggttgctctt ctcctcgagg acgccccgca tgatctgggc gccgtagacg aagatcgaca
     4801 tgtagatcag gaagaagagg atcccggtga tcgcgaggtt cgcgccctcg ttcttcgcgt
     4861 cctcgggcgt ctccttctcg gtcgcctcgc gcggatcgag cttcacggtg agccgggcct
     4921 tctcgtagac cgtcgggt
//
LOCUS       DAOTEZ010000333         4913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_12435_length_4913_cov_5.976739, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000333 DAOTEZ010000000
VERSION     DAOTEZ010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12435_length_4913_cov_5.976739"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="P5164_15085"
     CDS             complement(<1..257)
                     /locus_tag="P5164_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRY45267.1"
                     /translation="MSPETPGWVRDAVFYQVFPDRFARSERVAKPGNLEPWDSPPTPH
                     GFKGGDLLGLAERLDDLSALGVTALYLNPVFASAANHRYHT"
     gene            303..1616
                     /locus_tag="P5164_15090"
     CDS             303..1616
                     /locus_tag="P5164_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRY45268.1"
                     /translation="MRPAAVALAAGLAVPALAAPPAPRLPAILAPLGGEAEPLGELYR
                     ELHAAPELSMQETATAAKLAGRLRSLGFEVTEGVGGTGIVGVLRNGDGPVVMLRTDLD
                     ALPVEEKTGLAFASRATGKADDGTPIPVMHACGHDLHMTAWTGAATLLARAKGSWHGT
                     LVMVGQPAEEKGAGAAKMLKDGLFTRFPRPAFALALHVTPELPSGSVGVTPGPAFASV
                     DSVDLTIHGKGGHGAMPHRTVDPVLIAARTVVALQALVSREKDPFEPAVVSVGSFQAG
                     AKHNVIPPEARLKITVRAYREEVRQRLLDGIRRVATGESIAGGSPLAPDFSIVESTPG
                     VVNDAPLTERLRTAFGSALGAANVASFPPASVSEDFSYFGREGVPASLFWLGVAEPAA
                     LEKAKADGTSLPPLHSAEFAPDAGPAIRTGVTALVAGALELLPAR"
     gene            complement(2396..2767)
                     /locus_tag="P5164_15095"
                     /pseudo
     CDS             complement(2396..2767)
                     /locus_tag="P5164_15095"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
     gene            complement(2764..3192)
                     /locus_tag="P5164_15100"
     CDS             complement(2764..3192)
                     /locus_tag="P5164_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45269.1"
                     /translation="MPTTLAHRLELMRLLYRFKGLVEWELAKLAALDGETIPLELPEP
                     PAFVVRKLERKPPPIAPGREPATVAPAVPDTPSLPGAGAPDGSLADLSRPAGAPSHPL
                     QKTKDPTNLESCCRSADLPTGDYVALVDAATPYVRALAEE"
     gene            complement(3263..>4913)
                     /locus_tag="P5164_15105"
     CDS             complement(3263..>4913)
                     /locus_tag="P5164_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45270.1"
                     /translation="GRETSRVVARPVTSMPGWEIEPAVSPDGGLVAFASNASGSADIW
                     LVESSGGEPRQLTDVGGPGADAPDDLSPSWLPDGRSLLFSSDRVGRRSVFRVSFLGGS
                     PSLVVEDAAEPAVSGDGTLLAFTRTVGSGRYRRIWVAPFADPADARPITTDEDGLWDH
                     ARPAISPDGRRLCYADFRNLWIVDLPGGRPRPLTSDDAFDTEPRFSTDGRHVYFTSCR
                     GDVVAVWAVPASGGEAVRITGEAASETHPSFSRDGSVLVTATRVRDSDVAVLDRVAKT
                     VARISSTLSEETPVLEPDGRAVYFASNRAGRSNLWKQPLQAGRPFGSPIRLTSFDAGG
                     PATPSISRDGRFLAFFRVVSGNRDVWILPTAGGSARPLVGGPDREFHPALSSDARRLA
                     FVSSRGGVEHLYAVPLRDGAADGEPRPVTSGASADAFPAFSPDGSRIAFLRDGEIHLV
                     DAEGRGEPRRLTRDARPNHFAWDAGGDALVVAGRFSGRRLVLRRVRLDGVVEPIEPEV
                     ELGGELSSGYLSVSADGRFVAVQVGRLEADVWVRAPFTGGP"
BASE COUNT          669 a         1721 c         1951 g          572 t
ORIGIN      
        1 caggtgtggt aacggtggtt cgccgcagat gcgaagaccg ggttgaggta gagcgccgtc
       61 acgccgagcg ccgaaaggtc gtcgagcctc tcggcgaggc cgaggaggtc gcctcccttg
      121 aagccgtggg gcgtcggcgg ggagtcccac ggctcgaggt tccccggctt cgccacgcgc
      181 tcgcttcgcg cgaacctgtc ggggaagacc tggtagaaga cggcgtcgcg gacccagccg
      241 ggcgtctcgg ggctcacccc tccactatag tcggaggcgg gagagtctcc atggaggatc
      301 cgatgcgccc agccgccgtt gccctcgccg ccggcctcgc cgtccccgcc ctcgcggcgc
      361 ctcccgctcc gcgcctcccg gcgatcctcg cgccgctcgg cggcgaggcg gagccgctcg
      421 gagagctcta ccgcgagctt cacgccgcgc cggagctctc gatgcaggag acggcgacgg
      481 ccgcgaagct ggccgggcgc ctgcggtcgc tcggattcga ggtgaccgaa ggggtcgggg
      541 ggacggggat cgtcggcgtc ctccggaacg gggacggccc ggtcgtgatg ctccggaccg
      601 acctcgacgc cctgcccgtc gaggagaaga ccggcctcgc gttcgcgagc cgcgcgacgg
      661 gaaaggcgga cgacggcacg ccgatcccgg tcatgcacgc ctgcgggcac gacctccaca
      721 tgaccgcctg gaccggcgcg gcgacgctcc tcgcccgcgc gaaggggagc tggcacggga
      781 cgctcgtgat ggtcgggcag ccggccgagg agaagggggc cggggctgcg aagatgctga
      841 aggacgggct ctttacgcgg ttcccgcggc ccgccttcgc cctcgcgctc cacgtgacgc
      901 cggagctccc gtccggctcc gtcggcgtga cgccggggcc cgccttcgcc tccgtcgact
      961 cggtcgacct gacgatccac ggcaaggggg ggcacggcgc gatgccgcac cggaccgtcg
     1021 acccggtcct catcgcggcc cggacggtcg tcgcgctcca ggcgctcgtc tcccgcgaga
     1081 aggacccgtt cgagcccgcc gtcgtctcgg tcggctcctt ccaggccgga gcgaagcaca
     1141 acgtcatccc gcccgaggcc cgcctgaaga tcacggtccg cgcctaccgc gaggaggtga
     1201 ggcaacggct cctcgacggc atccgccgcg tcgcgacggg ggagtcgatc gccgggggct
     1261 cgccgctcgc gcccgatttc tcgatcgtcg agtcgacgcc gggagtcgtg aacgacgcgc
     1321 ccctgacgga gaggctccga acggccttcg gctccgcgct cggcgccgcg aacgtcgctt
     1381 ccttcccgcc ggcgagcgtg agcgaagact tctcctactt cgggagggaa ggcgtccctg
     1441 cctcgctctt ctggctcggc gtcgcggagc ccgcggcgct cgagaaggcg aaggccgacg
     1501 ggacgtcgct cccgccgctc cactcggccg agttcgcccc cgacgccgga cccgcgattc
     1561 ggacgggggt gacggcgctc gtcgccgggg cgctcgagct gctgcccgcg cgctgatctt
     1621 cggcggcgcg cgggtggcgc gcgcggcggt gccgcgccgt ccgcaggttc cagtcgcacc
     1681 agctcgccgg gacgaggtcc gggtcctgcc cgggcgcgcc gacgatcgcg gcggggcaga
     1741 cgtcgcactc gtcgagcgcg tcgcaggagg cgcaggggcc acgcggtgag cgacgcgccg
     1801 ccgaggcgac gagcgccggg aggccgagcc gacccgccgc gcggagcgcg agacgcgcgt
     1861 cgagcgccgg gtcgtccacc cgcggcccca cgagccgctc ccgaagcgcc tccgcgaggg
     1921 gcggaaggga cggcaggacg gacggggcga aagctccgca cggagcgatg cggccgtcga
     1981 cgtcgacgaa gagaacgtcg gcttcgccga ggctgcacgc ggggacgccg aacgggttcc
     2041 gagagcccgc cgaagggcgg agcggatcga aggccgagtc cccgttcccg ttcggaggga
     2101 gagaggcgac ctcctcgagg gcctggtcga ggaggtccgc ggaggcctcg ttccattccg
     2161 ggtcgggcgt ctggaccggc gcggcccgga tctcgcggac gcccgccgcg cggagctccg
     2221 cgaccgaacg gccgagcgcg ccgacgttcc cggagtcgac ggtcgcgcgc acgacgaggc
     2281 gctccgcgag gaggcgagga cggctccgcg cgaggcggag gagaagggag cggagtgcgg
     2341 cgccggtgcc ggcgccgcgg cgctcctgcg cggcgccgag gccgtcgcag gagagctcga
     2401 tcgtgacgcc cgccgcgtcg aggaacgcgg ccgtctcttc gtcgaggagg aggccgttcg
     2461 tcacgacgtg cacgtcgggc gcgaggcccg ggcgcgcgga tgccgcgagg agcgccagcg
     2521 ccctccggac gagcgccttc gcgaggagcg gctcgccgcc gaagaggacg agcttcggtc
     2581 gcgcgtggcc ggacgtggcc acgaggcgga cggcggcctc gagagcggcg gcgtccatct
     2641 cgcggggcgc gccgcgccgc tgcgggcagt aggcgcacgc gaggttgcac cgcgtcgtga
     2701 ggaggagcgt cacggcgcgc ggacccggga cgagcggccg gacggctcga ccgtcgatgc
     2761 gcgtcactcc tccgcgagcg cgcggacgta cggggtcgcc gcgtcgacga gcgcgacgta
     2821 gtccccggtg ggaaggtcgg ccgagcggca gcagctctcg aggttcgtgg ggtccttcgt
     2881 cttctgcaac gggtgcgacg gcgctccggc cgggcgtgag aggtcggcaa gcgagccgtc
     2941 cggcgcgccg gctcccggaa gggaaggagt gtcgggaacc gccggcgcca ccgtcgcggg
     3001 ctccctcccc ggcgcgatcg ggggaggctt ccgctcgagc ttccgtacca cgaaggccgg
     3061 aggctcgggg agctcgagcg gaatcgtctc gccgtcgagc gcggcgagct tcgcgagctc
     3121 ccactcgacg agtcccttga atcggtagag gagcctcatc agctcgaggc ggtgggcgag
     3181 cgtcgtgggc attccggtct cctttcgtcg tctgcgctcg gtcgagggtg cgccgggggc
     3241 ggtcccggcg cccgttcatc ggtcacggtc cgcccgtgaa gggagcccgg acccagacgt
     3301 cggcctccag gcggccgacc tggacggcga cgaagcggcc gtcggcgctc acggagagat
     3361 agcccgagga gagctccccg ccgagctcga cctccggctc gatcggctcg acgacgccgt
     3421 cgaggcggac gcggcggagg acgagcctgc gcccggagaa acgaccggcc acgaccagag
     3481 cgtcgccgcc ggcgtcccac gcgaagtggt tggggcgggc atcgcgcgtg agacgtcggg
     3541 gctcgccgcg cccctcggcg tcgacgaggt ggatctcccc gtcccggagg aacgcgatcc
     3601 gagagccgtc cggggagaag gccgggaagg cgtccgccga cgcgccggag gtgacgggcc
     3661 gaggctcgcc gtcggcggcc ccgtcgcgaa gagggacggc gtagagatgc tcgacgccgc
     3721 ctcgcgagga gacgaaggcg agccgccgcg cgtcggacga gagggccggg tggaactcgc
     3781 ggtcggggcc gccgacgaga ggacgggcgc tgccgccggc cgtcgggagg atccagacgt
     3841 cgcggttccc ggaaacgacc cggaagaacg cgaggaagcg tccgtcccgg gagatcgacg
     3901 gcgtcgccgg gcccccggcg tcgaaggacg tgaggcggat cggcgacccg aagggccggc
     3961 cggcctggag cggctgcttc cagaggttcg agcggccggc ccggttggag gcgaagtaga
     4021 cggcccggcc gtccggctcg aggacgggcg tctcctcgct cagcgtgctc gagatcctcg
     4081 cgacggtctt cgcgacgcgg tcgaggacgg cgacgtcgga atccctcacg cgcgtcgcgg
     4141 tgacgaggac gctcccgtca cgcgagaacg agggatgcgt ctccgaggcc gcctcgccgg
     4201 tgatccggac ggcttcgccc ccggaggccg ggacggccca gaccgcgacg acgtccccgc
     4261 ggcaggaggt gaaatagacg tgacgaccgt cggtcgagaa gcgcggctcg gtgtcgaacg
     4321 cgtcgtcgga ggtgagcggg cggggcctgc cgccgggcag gtcgacgatc cagaggttcc
     4381 ggaagtcggc gtagcagagc cttcggccgt cgggggagat cgcgggccgg gcgtggtccc
     4441 agagaccgtc ctcgtccgtc gtgatcggcc gggcgtcggc ggggtcggcg aacggggcga
     4501 cccagatccg ccggtatcgg ccgcttccga cggtgcgggt gaacgcgagg agtgtcccgt
     4561 cgccgctgac ggccggctcc gcggcgtcct ccacgacgag gctcgggctc ccgccgagga
     4621 aggagacgcg gaagaccgag cggcggccga cccggtccga ggagaagagg agcgaacgcc
     4681 cgtcggggag ccaggacggg gagaggtcgt cgggggcgtc ggcgccgggg cccccgacgt
     4741 ccgtcagctg gcggggctcg ccgccggagc tctcgacgag ccagatgtcc gcgctgcccg
     4801 aggcgttcga ggcgaacgcg acgagcccgc cgtccggaga cacggccggc tcgatctccc
     4861 agccgggcat cgaggtgacg ggtcgcgcga cgacccggga ggtctcgcgg ccg
//
LOCUS       DAOTEZ010000334         4909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_12446_length_4909_cov_6.865060, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000334 DAOTEZ010000000
VERSION     DAOTEZ010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12446_length_4909_cov_6.865060"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1855
                     /gene="ybaL"
                     /locus_tag="P5164_15110"
     CDS             149..1855
                     /gene="ybaL"
                     /locus_tag="P5164_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010089790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HRY45271.1"
                     /translation="MPHEISLITTIAAGFGLALVFGLIASKLRIPAIVGYLVAGMIIG
                     PGTPGFVADVGLAGQLAEIGVMLLMFGVGLHLSMGELLSVKRIAVPGAIVQIALATLF
                     GMGAAKLWGWSTGASLVFGFSLATASTVVLLRALESRGILESQDGRIAIGWLVVEDLA
                     CVLALVLLPPLATLLGGNAPAGAPAGGSLLAAVAITLGKVALFVVLMLVVGKRVFPRI
                     LFWVARTGSRELFTLCVLATAIGVAYGSAKLFDVSFALGAFFAGMMMRESEFAHRAAD
                     ESLPLRDAFSVLFFVSVGMLFDPRVLLEQPVKVLVVVAIIMIGKTLGAVGLVLALRYP
                     LHTALTVGAALAQIGEFAFIIAVLGRDLGLLPAAGHSLILVGSIISIGLNPVVFSTVE
                     PIAEWLKRHSALARRLAALEHETAALPTTVDEALLTGQVVLVGYGRVGRRIAEALSER
                     RVPCVVAEMSRDAVADLREKGIPAVSGDASDPTVLVQAHVATAAMLVVATPDPVKVRK
                     MVEVARTLNPSIEIVLRTHTEEEAELLREEKAGKVFMGEHELALAMAGHVLARMDHPS
                     AA"
     gene            2056..3042
                     /gene="hflK"
                     /locus_tag="P5164_15115"
     CDS             2056..3042
                     /gene="hflK"
                     /locus_tag="P5164_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014019345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HRY45272.1"
                     /translation="MEIQFPDGTRLRTPSIPVRLLARGALGVVAFVVLFSSWFTIEPE
                     EAGLVLRFGKYARTVSPGLHLKLPSPIERVVKVPVERQLKEEFGFRTDASASPRSTYS
                     QEDLSSESLMLTGDLNVAVVEWTAQYRVRDPYKFLFKVRSVQKTFRDMNETVMREIIG
                     DRSVNEVLTVGRQEIAAEVEQRLQDLCDQYETGIKVEQIVLQDVNPPDPVKPSFNEVN
                     QAQQEREKLINQARADYNQIIPRASGQAQQTIEQAEGFATDRVNRARGDAELFVKVHA
                     AYQRAPEVTRRRMHLETMGEIYPKVKRKVVLDEKLKGVLPLLPLAGAPEVKP"
     gene            3039..3977
                     /gene="hflC"
                     /locus_tag="P5164_15120"
     CDS             3039..3977
                     /gene="hflC"
                     /locus_tag="P5164_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HRY45273.1"
                     /translation="MKEARIASLVLAAVLAVVVLSSSLYTVAETQQVIVTQFGKPVGK
                     PITTPGLKMKAPFIQVVHAFEKRFMEWDGDPNQIPTKDKRFIWVDCYARWRITDPLLF
                     FQRLRDERGAQSRLDDILDGETRNTIAKHELIDIVRSTNRPFVVGDDMADLSQPEPAE
                     KVEYGREKLTEEVLANARKRTHDLGIEILDFRIKQINYVEAVRQEVYARMISERKRIA
                     ERYRSEGAGEAARIAGEKERQLRVIESEAYRKAQEIRGKADAQAADIYAAAYNRDPDF
                     YRFLKSMTTLRESVDPETTLVLSTDGDLLRYLNAAK"
     gene            complement(4006..4608)
                     /locus_tag="P5164_15125"
                     /pseudo
     CDS             complement(4006..4608)
                     /locus_tag="P5164_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738851.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
     gene            4647..>4909
                     /locus_tag="P5164_15130"
     CDS             4647..>4909
                     /locus_tag="P5164_15130"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRY45274.1"
                     /translation="MPRLRLLTAGESHGPLLTALLEGLPAGLRLDVAAIDADLARRQH
                     GYGRGGRMKIERDRVRFTGGLRGGETLGSPLAMTVENLDHGVW"
BASE COUNT          724 a         1773 c         1698 g          714 t
ORIGIN      
        1 cggcgacgag gcgcgccacg cccgcgccct cctcgagacg gtccgcgccg aggcgcagcg
       61 gccggacccg gggacgcgga cgcggtaaag atcccggccg tctccccctc cccgccctcc
      121 gttaccatcc ggcaggaagc acgaacggat gcctcacgag atcagcctca tcacgaccat
      181 cgccgccggc ttcgggctcg cgctcgtctt cggcctgatc gcctcgaagc tgcgcatccc
      241 cgcgatcgtc gggtacctcg tcgcggggat gatcatcggc ccggggacgc cgggcttcgt
      301 cgccgacgtc gggctcgcgg ggcagctcgc cgagatcggc gtgatgctcc tgatgttcgg
      361 ggtcgggctc catctctcga tgggggagct cctctccgtg aagcggatcg cggtgccggg
      421 ggcgatcgtc cagatcgcgc tcgcgacgct cttcgggatg ggggccgcga agctctgggg
      481 ctggagcacc ggcgcctcgc tcgtcttcgg cttctcgctc gcgacggcga gcaccgtcgt
      541 cctcctccgc gccctcgaga gtcgcgggat cctggagtcg caggacgggc ggatcgcgat
      601 cggctggctc gtcgtcgagg acctcgcctg cgtcctcgcc ctcgtcctcc tcccgccgct
      661 cgcgacgctc ctcggcggga acgcgccggc cggcgcgccg gccggcggga gcctcctcgc
      721 ggcggtcgcg atcacgctcg ggaaggtcgc cctcttcgtc gtcctgatgc tcgtcgtcgg
      781 caagcgggtc ttcccgcgaa tcctcttctg ggtggcccgg accggctcgc gcgagctctt
      841 cacgctctgc gtcctggcga cggcgatcgg cgtcgcctac ggctcggcga agctcttcga
      901 cgtctccttc gccctcgggg cgttcttcgc cgggatgatg atgcgcgagt cggagttcgc
      961 gcaccgcgcg gccgacgaat ccctgccgct ccgcgacgcc ttctccgtcc tcttcttcgt
     1021 ctcggtcggg atgctcttcg acccgcgcgt ccttctcgag cagccggtga aggtgctcgt
     1081 cgtcgtcgcg atcatcatga tcggcaagac gctcggagcg gtggggctcg tcctcgcgct
     1141 gcgctacccg cttcacaccg cgctgacggt cggggccgcg ctcgcgcaga tcggcgagtt
     1201 cgcgttcatc atcgcggtcc tcgggcgcga cctcggcctg ctcccggcgg cggggcacag
     1261 cctcatcctc gtgggctcga tcatctccat cggcctgaac cccgtcgtct tctccaccgt
     1321 cgagccgatc gccgagtggc tgaagcggca ctcggccctc gcccgcaggc tggccgccct
     1381 ggagcacgag accgccgcgc tcccgacgac ggtcgacgag gcgctcctca ccggccaggt
     1441 ggtcctcgtc ggctacgggc gggtcgggcg gcggatcgcc gaggcgctct cggagcggag
     1501 ggtcccgtgt gtcgtcgccg agatgagccg ggacgcggtt gccgaccttc gcgaaaaggg
     1561 gatcccggcc gtctcgggcg acgcctccga tccgacggtc ctcgtccagg cccatgtggc
     1621 gacggcggcg atgctcgtcg tcgccacgcc cgacccggtg aaggtccgga agatggtgga
     1681 ggtcgcgcgg actctgaacc cgtcgatcga gatcgtcctc cggacccaca cggaggagga
     1741 ggccgagctg ctacgcgagg agaaggccgg gaaggtcttc atgggcgagc acgagctggc
     1801 gctcgcgatg gcggggcacg tcctcgcccg gatggaccac ccctcggctg cctgagcgcg
     1861 cccggcgtcc gtcccggggc ccggcgacgc ccggggcgtg gggcgggagc ccggtttcgg
     1921 gagaagcccc gtcgaggcgc atcgaaaagc gccgaatccg cacgttccgg ggaatctccc
     1981 ctcgatcggc cgcgttgcat cgtttcggcg tgcgtggcgg caccggccgg ccggcgccct
     2041 gcgaaaggag acccgatgga gatccaattc cccgacggga cgcggctccg gaccccgagc
     2101 atcccggtcc gactcctggc ccgcggagcc ctcggcgtcg tggcgttcgt cgtcctcttc
     2161 tcgtcctggt tcacgatcga gccggaggag gcggggctgg tcctccggtt cgggaagtac
     2221 gcccggaccg tctccccggg cctccacctg aagctcccga gcccgatcga gagggtcgtg
     2281 aaggtccccg tcgagcggca gctgaaggag gagttcggct tccggacgga tgcctccgcc
     2341 tccccccgct cgacctactc gcaggaggac ctctcctccg agtcgctgat gctgacgggc
     2401 gacctgaacg tggcggtcgt ggagtggacg gcccagtacc gggtccgcga cccgtacaag
     2461 ttcctcttca aggtccggag cgtccagaag acgttccgcg acatgaacga gacggtcatg
     2521 cgcgagatca tcggcgaccg gagcgtgaac gaggtcctga ccgtcggccg gcaggagatc
     2581 gccgccgagg tcgagcagcg gctgcaggac ctctgcgatc agtacgagac cggcatcaag
     2641 gtcgagcaga tcgtcctgca ggacgtgaac ccgcccgacc cggtcaagcc ctcgttcaac
     2701 gaggtcaacc aggcccagca ggagcgcgag aagctgatca accaggcccg cgccgactac
     2761 aaccagatca tcccgcgcgc gagcggacag gcccagcaga cgatcgagca ggccgagggc
     2821 ttcgcgaccg accgcgtcaa ccgggcccgc ggcgacgccg agctcttcgt gaaggtccac
     2881 gccgcctacc agcgcgcccc cgaggtgacc cggcgccgga tgcacctcga gacgatgggc
     2941 gagatctacc cgaaggtgaa gaggaaggtc gtcctcgacg agaagctgaa gggggtcctc
     3001 cccctcctgc ccctcgcggg cgccccggag gtgaagccgt gaaggaagcc cggatcgcct
     3061 ccctcgtcct cgccgccgtc ctcgcggtcg tcgtcctctc gagctcgctc tacacggtgg
     3121 ccgagacgca gcaggtcatc gtcacccagt tcggcaagcc ggtcggcaag ccgatcacga
     3181 cgcccggcct gaagatgaag gcgccgttca tccaggtcgt ccacgccttc gagaagcggt
     3241 tcatggagtg ggacggcgac ccgaaccaga tcccgacgaa ggacaagcgg ttcatctggg
     3301 tcgactgcta cgcccgctgg cgcatcaccg acccgctcct cttcttccag cgcctccgcg
     3361 acgagcgggg cgcgcagtcg cgtctcgacg acatcctcga cggcgagacc cggaacacga
     3421 tcgccaagca cgagctgatc gacatcgtga ggtcgacgaa ccgccccttc gtcgtcggcg
     3481 acgacatggc cgacctttcg cagccggagc cggccgagaa ggtggagtac ggccgcgaga
     3541 agctgaccga ggaggtcctc gcgaacgctc ggaagcggac ccacgacctc gggatcgaga
     3601 tcctcgactt tcggatcaag cagatcaact acgtcgaggc ggtgaggcag gaggtctacg
     3661 cccggatgat ctccgagcgg aagcggatcg ccgaacgcta ccgctccgag ggggcgggcg
     3721 aggccgcccg catcgcgggc gagaaggagc ggcagctccg ggtcatcgag tcggaggcgt
     3781 accggaaggc gcaggagatc cggggcaagg ccgacgcgca ggccgccgac atctacgccg
     3841 ccgcctacaa ccgcgacccc gacttctacc ggttcctgaa gtcgatgacg acgctccggg
     3901 agagcgtcga cccggagacg acgctcgtcc tctccaccga cggcgacctc ctccgctacc
     3961 tgaacgccgc gaagtgatcg cggacgacgc cggcccggac ctcctctacc gggccggcgt
     4021 cgcgccctgc ggagccggcg gctccatctc tacccagagg tcggagagga cgatctcgag
     4081 ctcctcgaac ggctcggccc ggacgctctc cgtcccctcg tgcgccgaga ggaggaccca
     4141 ccgcccctcg gcgagccgct agacctcgag cgttcggacg cccggatcga cgagccaggc
     4201 gtgcccgacc ccctcggccg cgaaaacgcg catcttcttc acacggtcga agctcgccgt
     4261 cgacggcgag aggacctcgc agacccagtc gggagcgagg gtgaagaagg cggtcggcgg
     4321 aatcgccggg agccggctcc gccgccagcc ggcgaggtcg ggaacgagga cgttccggcc
     4381 gaggtggatc tcgggctcga agacgatcca ccagccgccg gggccgccgc gcccccgctg
     4441 gtaggcgccg ccgatgtcca tgccgagcgt cgaggaggcc gccgcgtggg gattcgcggg
     4501 gcggggtgtc gcgaagagct cgccatcgac gatctgcccg ttgacgtgcg gggggagcgc
     4561 gaggatgtcc tcgtacgtcg cgggtcgctt ccgggaggtg tccgacacgg cgaggcgaga
     4621 ttaccgctcc gctagactcc ccttccatgc cgcgcctccg ccttctgacc gccggcgagt
     4681 cccacggccc gctcctgacc gcgctcctcg aggggctccc ggccgggctg cgactcgacg
     4741 tcgcggcgat cgacgccgac ctcgcgcgcc gccagcacgg ctacggccgc ggcgggcgga
     4801 tgaagatcga gcgcgaccgg gtgcggttca cgggcggcct gcgcggcggc gagacgctcg
     4861 gctctccgct cgcgatgacg gtcgagaacc tcgaccacgg cgtctggga
//
LOCUS       DAOTEZ010000335         4883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_12533_length_4883_cov_7.490472, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000335 DAOTEZ010000000
VERSION     DAOTEZ010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12533_length_4883_cov_7.490472"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="P5164_15135"
     CDS             complement(<1..185)
                     /locus_tag="P5164_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding-motility protein MglA"
                     /protein_id="HRY45275.1"
                     /translation="MTFINYAAREINCKIVYYGPGLCGKTTNLQYIYDRTNPAAKGKL
                     ISLATETDRTLFFDFLPL"
     gene            complement(262..756)
                     /locus_tag="P5164_15140"
     CDS             complement(262..756)
                     /locus_tag="P5164_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HRY45276.1"
                     /translation="MTDFVLFDEEFQQIKEVIGRLKADAQAKVVFIVDKNGQQIAAQG
                     DLESLDTTSLASLTAGNVAATDGLAKLIGEKEFPVLFHEGEKDNVYIAIVDQRVILVI
                     IFDEHSSLGLVRLRVRKATQDLERIFVSIQRKVEEEKERRHEGVDSPFAEITDEDIDS
                     LFRE"
     gene            complement(807..1400)
                     /gene="recR"
                     /locus_tag="P5164_15145"
     CDS             complement(807..1400)
                     /gene="recR"
                     /locus_tag="P5164_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRY45277.1"
                     /translation="MTSLPALARLVAALERLPGIGPKSARRIAHFLLTAPPDASEGLS
                     AAIFEARERTRSCSVCHALTEQDPCAFCVDPARDGALLAVVEEPFDVEVLERTREFRG
                     RYHVLGGALAPLRGIGPDDLHVASLLERAASGVAEVVLATNPNVEGEATALYLARRLK
                     PLGVRVTRIAFGLPVGAAIEFADEVTLGRSLSGRREV"
     gene            complement(1397..3166)
                     /gene="dnaX"
                     /locus_tag="P5164_15150"
     CDS             complement(1397..3166)
                     /gene="dnaX"
                     /locus_tag="P5164_15150"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRY45278.1"
                     /translation="MTTNAFVPLARKWRPRTFADVVGQAEIVRALTNALTQDRMAHAY
                     LFSGPRGVGKTTSARLLAAGINCRTAAGPTPSPCGTCESCQEVLEGRSIDALEIDGAT
                     HGKVDQARDLIEIVGYAPVRDRRKVFIIDEVHAISAAAFQALLKTLEEPPSHAVFILA
                     TTERHKIPATILSRCQRFDFRRLTDAEVADRLSEIASREGYSVSPDEKPAPWVEPAAL
                     AALAAAATGSLRDGLSLLDQAVAQTGGRVTAADVARLLGSPDRTALVALLASVLAADR
                     AAVLRGAAELDAAGSDPRATAHDLTLLVRGTVRFAADPSAPAIAGISEDGSAALRELA
                     RATPYSTLLRVLTFLTEAEGMLRRSDVPALALEVLLLRLAELPRLVPIEEVLSSGAGL
                     PAVGPVGPIAAPAPAAPPSFTPRTPAPAEVRRAPAPAAKAPAAPEAPRFVGLTPLEVA
                     EPEPEAPADAATAFREAVEKRHGNLGAALEDVSVLVEGRVVRLVLDPPSPPLAKRLGE
                     PAMKKVLDEAAFAVLGAKASVTVESAEPAGGDLNAAAAAADPATLERENLKKRAATDE
                     RVKKILDMFDGEIAEVKPDERKA"
     gene            complement(3188..3286)
                     /gene="ffs"
                     /locus_tag="P5164_15155"
     ncRNA           complement(3188..3286)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="P5164_15155"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3369..3458)
                     /locus_tag="P5164_15160"
     tRNA            complement(3369..3458)
                     /locus_tag="P5164_15160"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3422..3424),aa:Ser,seq:gga)
     gene            3609..4079
                     /locus_tag="P5164_15165"
     CDS             3609..4079
                     /locus_tag="P5164_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45279.1"
                     /translation="MARILPRLLPSALAAILAILATAPAAAAGKLAEETVDVPREHRV
                     PLEFAHEKGAIFALESHNDPKADDLEEARQKDPDDKTWVLLRFYYRNDGWTRQRVKVR
                     ALLLDADGGVLADVGRSTKLAKQKPEGTFNVPMKVKTLDWGKAAKMKLLVTFLD"
     gene            4191..4280
                     /locus_tag="P5164_15170"
     tRNA            4191..4280
                     /locus_tag="P5164_15170"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4225..4227,aa:Ser,seq:cga)
     gene            complement(4330..>4883)
                     /locus_tag="P5164_15175"
     CDS             complement(4330..>4883)
                     /locus_tag="P5164_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45280.1"
                     /translation="CKLDLVAHAFGAVGYAECPRCSGLFLRREAFEAVTKDADTRAKV
                     RLAEPPAEAAAAPLGKAASGVLPAVRYRPCPTCAKLMNRTNYGGGSGIVLDGCRDHGL
                     WFDKGELAAIVDFLEKGGWDRIRQRERERLAEEVRSLEFRKNVGAATALPSSGWEREV
                     QGMRTIGGIVDLAGSLAGWFLKR"
BASE COUNT          685 a         1715 c         1801 g          682 t
ORIGIN      
        1 agcgggagga agtcgaagaa gagcgtccgg tcggtctcgg tcgccaggga gatcagcttc
       61 cccttcgccg cggggttcgt ccggtcgtag atgtactgca ggttcgtcgt cttcccgcag
      121 aggccgggac cgtagtagac gatcttgcag ttgatctctc gggcggcgta gttgatgaag
      181 gtcatcgctt cggcaccctc agacaatcct ccgcggcgcg gggggttccc tcaggacgtc
      241 gtgcgcgcgt gcccgtcatc gtcactcccg gaagaggctg tcgatgtctt cgtccgtgat
      301 ctcggcgaac ggggagtcga cgccctcgtg ccgccgctcc ttctcctcct cgaccttccg
      361 ctggatcgag acgaagatcc gctcgaggtc ctgcgtcgcc ttgcggacgc ggagcctcac
      421 gaggccgagg gacgagtgct cgtcgaagat gatgacgagg atgaccctct ggtcgacgat
      481 ggcgatgtag acgttgtcct tctcgccctc gtggaagagg accgggaact ccttctcgcc
      541 gatgagcttg gcgaggccgt ccgtggcggc gacgttcccg gccgtcaggg aggcgaggga
      601 ggtcgtgtcg agcgactcga ggtctccctg ggcggcgatc tgctggccgt tcttatcgac
      661 gatgaagacg accttcgcct gggcgtccgc cttgagccgc ccgatgactt ccttgatctg
      721 ctggaactcc tcgtcgaaaa ggacgaaatc cgtcatatga cgccgatcct cgctagtgta
      781 accgatgccc ggcaccgcac ttccggctag acctcccgcc gtcccgagag ggagcggccg
      841 agggtgacct cgtcggcgaa ctcgatcgcc gccccgacgg ggaggccgaa ggcgatgcgg
      901 gtcacgcgca ccccgagcgg cttgagccgc cgggcgaggt agagggcggt ggcctctccc
      961 tcgacgttcg ggttcgtggc gaggacgacc tcggcgacgc ccgaggccgc ccgctcgagg
     1021 aggctcgcga cgtggaggtc gtcggggccg atgccgcgaa gcggcgcgag cgccccgccc
     1081 aggacgtgat agcggccccg gaactcgcgc gtccgctcga gcacctcgac gtcgaacggc
     1141 tcctccacga cggccaggag cgctccgtcc cgcgcggggt cgacgcagaa cgcgcacggg
     1201 tcctgctcgg tgagggcgtg gcagacggag caggagcgag tccgctcgcg cgcctcgaag
     1261 atcgcggccg agagcccctc ggacgcgtcg ggaggcgcgg tcaggaggaa gtgggcgatg
     1321 cgccgcgcgc tcttcgggcc gattcccgga aggcgctcga gcgccgcgac gaggcgcgcg
     1381 agcgccggga gcgaggtcac gccttccgct cgtcgggctt cacttcggcg atctcgccgt
     1441 cgaacatgtc gaggatcttc ttcacgcgct cgtccgtcgc ggcccgcttc ttcaggttct
     1501 cgcgctcgag cgtcgccggg tccgcggccg ccgcggcggc gttcaggtcg ccgccggccg
     1561 gctcggcgct ctccacggtg acggacgcct tcgcgccgag gacggcgaag gcggcctcgt
     1621 cgaggacctt cttcatggcc ggctcgccga gccgcttcgc gagcggcggg ctcggcgggt
     1681 cgaggacgag gcggacgacc cgcccctcga cgaggacgga gacgtcctcg agggccgcgc
     1741 cgaggttccc gtggcgcttc tccaccgcct cgcggaacgc cgtggccgcg tcggccgggg
     1801 cctccggctc cggctcggcg acctcgagcg gggtgagacc gacgaaccgg ggcgcctcgg
     1861 gcgccgccgg ggccttcgcc gccggcgcgg gcgccctgcg gacctccgcc ggagcgggcg
     1921 tacggggcgt gaaggagggg ggcgcggcgg gcgcgggcgc tgcgatcggg ccgaccgggc
     1981 cgacggcggg gaggcccgcg ccggaggaga ggacctcctc gatcgggacg aggcgcggga
     2041 gctcggccag gcggaggagc agcacctcga gggccagggc gggaacgtcg ctccgacgga
     2101 gcatcccctc cgcctccgtc aggaacgtca ggacgcggag cagcgtggag tagggcgtcg
     2161 cccgcgcgag ctcgcggagc gcggccgagc cgtcctcgga gatgccggcg atcgcgggag
     2221 ccgacgggtc ggccgcgaag cggaccgtcc cgcggacgag gagcgtcagg tcgtgcgccg
     2281 tcgcccgcgg gtccgacccg gcggcgtcca gctccgccgc gccgcgcagg accgccgctc
     2341 ggtcggccgc gaggaccgag gcgaggaggg cgacgagggc cgtccggtcg ggcgagccga
     2401 ggagtcgggc gacgtcggcc gcggtgacgc gcccccccgt ctgcgcgacc gcctggtcga
     2461 ggagcgaaag gccgtcgcga aggctcccgg tcgccgcggc ggcgagcgcc gcgagcgcgg
     2521 ccggctccac ccagggggcg ggcttctcgt cggggctgac ggagtacccc tcgcgggagg
     2581 cgatctcgga gagccggtcg gcgacctccg cgtcggtgag gcgacggaag tcgaaccgct
     2641 ggcagcgcga caggatcgtg gcgggaatct tgtgccgctc ggtcgtcgcg aggatgaaga
     2701 cggcgtgcga cggcggctcc tcgagcgtct tcaggagcgc ctggaaggcg gccgcggaga
     2761 tcgcgtggac ctcgtcgatg atgaagacct tccggcggtc cctcaccggc gcgtagccga
     2821 cgatctcgat caggtcgcgg gcctggtcga ccttgccgtg cgtggcgccg tcgatctcga
     2881 gcgcgtcgat cgagcgcccc tcgaggacct cctggcacga ctcgcacgtc ccgcacgggc
     2941 tcggcgtcgg tccggcggcc gtccggcagt tgatgccggc cgccaggagt cgcgccgagg
     3001 tcgtcttccc gacgccgcgc ggccccgaga agaggtacgc gtgcgccatc cggtcctggg
     3061 tgagggcgtt cgtcagggcg cgcacgatct cggcctgacc gacgacgtcg gcgaaggtcc
     3121 gggggcgcca cttgcgggcg agcgggacga aggcgttcgt cgtcatcgaa gaagccgagc
     3181 ccctcgatga gtccgggtga tccgcggcgc ccgagccgtt cctcttagtg ctgcttcctt
     3241 ccggacctga cacggttcga gggagctcgt cgcgcgggac ccgaactcac ccaggggccc
     3301 gggccgggga ttctagtcta cggagccggg gcgcggccgg aatcccgggc gcgcccgcgg
     3361 gacgaacgtg gcggacaggg tgggattcga acccacggta gcctttcgac tacacacggc
     3421 ttccaaccgt gctccttaaa ccgctcggac acctgtccac gtccgcgggg acgggaactc
     3481 tgccccgccg gcgccgatcc gtcaagcgcc atgacggcgc cggaccggct ccggccgcgc
     3541 ccggggccgc cgaaaagtcg ccccggtcgg caaacgcggg ggcggccgga gacgtagaac
     3601 ggaacgtgat ggcccgtatc ctcccgcgcc tcctcccgtc cgccctcgcc gcgatcctcg
     3661 cgatcctcgc cacggctcct gccgccgccg ccgggaagct ggccgaagag acggtcgacg
     3721 tcccccggga gcaccgggtc cccctcgagt tcgcccacga gaagggggcg atcttcgccc
     3781 tcgagtcgca caacgacccg aaggcggacg acctcgagga ggcgcgccag aaggacccgg
     3841 acgacaagac gtgggtcctc ctccggttct actaccgcaa cgacggctgg acgcggcagc
     3901 gggtcaaggt ccgggcgctc ctcctcgacg ccgatggggg cgtcctggcc gacgtcggcc
     3961 gctccacgaa gctcgcgaag cagaagcccg aaggcacctt caacgtcccg atgaaggtga
     4021 agacgctcga ctgggggaag gcggcgaaga tgaagctcct ggtcacgttc ctggactagc
     4081 ggctttccgg gagcctctcg agcggccgag gcgggaccct cgcgggccgg ggccttcccc
     4141 gttcggcagg catgcgctat cctctcgccc ccgctccgag gccgaaacgc ggtgaggtgc
     4201 tggagtggtt tatcaggcca gtctcgaaaa ctggtgtagt cgaaaggcta ccgtgggttc
     4261 aaatcccacc ctcaccgcca tctgattccc gggaagcacg acccgccggc gacggcgggc
     4321 gtttccgtct cagcgcttca ggaaccagcc cgcgagcgac cccgcgaggt cgacgatccc
     4381 gccgatcgtc cgcatcccct ggacctcccg ctcccagccc gaggacggca gggccgtcgc
     4441 cgccccgacg ttcttccgga actccaggct ccggacctcc tcggcgagcc tctcgcgctc
     4501 gcgctggcgg atccggtccc agcccccctt ctcgaggaag tcgacgatcg cggcgagctc
     4561 ccccttgtcg aaccagaggc cgtggtcgcg gcagccgtcg aggacgatcc ccgagccgcc
     4621 gccgtagttc gtccggttca tcagcttcgc gcaggtcggg caggggcggt agcggaccgc
     4681 gggaaggacg ccgctggcgg cttttccgag cggggccgcg gcggcctccg cgggaggctc
     4741 cgcgaggcgg accttcgccc gcgtatcggc gtccttcgtc accgcctcga acgcctcgcg
     4801 cctgaggaag aggcccgagc agcgcggaca ctcggcgtag ccgaccgcgc cgaaggcgtg
     4861 ggccacgagg tcgagcttgc agt
//
LOCUS       DAOTEZ010000336         4880 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_12545_length_4880_cov_7.162487, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000336 DAOTEZ010000000
VERSION     DAOTEZ010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4880
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12545_length_4880_cov_7.162487"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /gene="lpxC"
                     /locus_tag="P5164_15180"
     CDS             <1..767
                     /gene="lpxC"
                     /locus_tag="P5164_15180"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HRY45281.1"
                     /translation="RTDVGAVLPALAEEAGRLDYATSLGERGREVGTVEHLLSAAAGL
                     GLDNLTVEIDGPELPILDGSSAPWVAEIHSAGLTTLASAARPFAVPKTLSVHGSDGKW
                     IEIRPAKELRISYSIDFPHPAVGRQSISLVLTPETYAAHLAPARTFGFLAEYEYMKSK
                     GLARGASEDNCIVVGESAVLNGGLRFPDEFVRHKVLDLVGDLALVGRPVVGHVVAHRA
                     GHALHTALAKEIRQALAGEHLRRVEPAPESVALAGR"
     gene            799..1374
                     /locus_tag="P5164_15185"
     CDS             799..1374
                     /locus_tag="P5164_15185"
                     /inference="COORDINATES: protein motif:HMM:NF025692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HRY45282.1"
                     /translation="MRPRPLVVAAALIALAAAFPAPGADPDRLRIPQVETTFQGRDVA
                     VAAGLSRPLPEDVRKRLASGLPTTVTWQVRLFVSRRKWWDDPLDERRYAVTATYRPVG
                     GDFAVERRLDGRLLETVVLPSLAEAEAALSALAGLPCFRMGPALAGPTLAVRVRCVYG
                     SRLALGFLPTAVGTSWRKSPVFRWREPGGTP"
     gene            1371..3569
                     /locus_tag="P5164_15190"
     CDS             1371..3569
                     /locus_tag="P5164_15190"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY45283.1"
                     /translation="MKAAWARDNRVLIGLGLLLGATFAGIYALVLRSRALAAPAATNR
                     VLLFVLTYIVVVLILALLLVIGRSVFRLLLDTRRGVFGSRFQVRIVLTNVGLALLPIA
                     LLILPTTGLLQRSVDVWFAPPVTQTIESGREVVEIVRRRAAERELRVAARLAATLPEA
                     RGDTARLALLAAAREESGLDYVELRPAGNGARPSVLAVSAPRWPLRDVPEPSSEWVSA
                     ARARGSVRRVDSVDGGGLVGRTVIADRAGLLVLGTYEPPAEAAPLRALSRSTSAFAQL
                     EAERGSIQAVQVLLFLLLALLVLLAAVWVGLVLARRVTRPVAALARSVRRVGAGDFTA
                     RVEVEGADEIATLGSAFNAMTEELRGSREQLVSANAALRSAYARMDEERLRIRTILAH
                     LDVGVLAFGPAPGEDGGASRLGGMNDAAMRMLRTPDAPAGTTLAELLPGDWAAPLQEL
                     LAGAFRGTGSREGTLSLSPPGAREPMVLEVHVTGVAGEGPERTAWVVTLEDTTALVRA
                     ERAAAWEEAARRMAHEIKNPLTPVRLAAERIRRKARGSAGCDPELAKVVEEGADTIVQ
                     EVRSLAALVDAFHRFARLPETRLGELDLGAVVAQVVKLYEGTKAGVRVAAEPAPDLPA
                     VRGDAEQVKRALVNLVDNAVAATPPGGAVSVRASVDGGLARVVVADDGPGIPPEERDL
                     VFEPTFSTKANGYGLGLSITSRIAAEHRGRVILEENAPRGCRFVFEWPAA"
     gene            3592..>4880
                     /locus_tag="P5164_15195"
     CDS             3592..>4880
                     /locus_tag="P5164_15195"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRY45284.1"
                     /translation="MSAPGPRGATVLVCDDAAGIRKTLAQILGDEGYRVETFEDGGSV
                     LGRLGEPKLPRPAALFLDVWLPDVDGLSVLDQLRARGHDLPVVMISGHGTVETAVQAV
                     KRGADDFLEKPLALERVLLTLEKALERSRLARERDVLAREVARQRERLEEEETLLGEG
                     PAMTRLREELRRAGASDARVLISGESGVGKEVAARTLHRLSPRAGGPFVDVNGAAIPE
                     DLIESELFGHVKGSFTGATEDRKGKWEAADGGTLFLDEIGDMSPRTQAKILRAIQDGK
                     ISRVGATRTIETDVRIVAATNRDLPKMIAAGTFREDLYFRLAVVPIRVPTLAERPEDI
                     PALVAHFAGKLARRRGRRPPTFSAEALEELKRRPWPGNVRELQNVVERALLLADGAVI
                     GADDLPGDVLRLPRTGGDAGDGLMPGQTLADARDAFER"
BASE COUNT          570 a         1817 c         1853 g          640 t
ORIGIN      
        1 tccggacgga cgtcggcgcc gtcctcccgg cgctcgccga ggaggcgggc cgcctcgact
       61 acgcgacctc gctcggcgag cgcggccgcg aggtggggac ggtcgagcac ctcctctccg
      121 ccgccgccgg gctcggcctc gacaacctga cggtcgagat cgacggcccc gagcttccga
      181 tcctggacgg cagctccgcc ccttgggtcg ccgagatcca ctcggccggc ctcacgacgc
      241 tcgcctccgc ggcgcggccg ttcgccgtcc cgaagactct ctcggtccac ggctccgacg
      301 ggaagtggat cgagatccgt ccggcgaagg agctgcgcat ctcctactcg atcgacttcc
      361 cgcacccggc cgtcggccgg cagtcgatct cgctcgtcct gacgcccgag acgtacgccg
      421 cgcacctcgc gccggcgcgg acgttcgggt tcctggccga gtacgagtac atgaagtcga
      481 aggggctggc ccgcggcgcg agcgaggaca actgcatcgt cgtcggagag agcgccgtcc
      541 tgaacggcgg cctccgcttc cccgacgagt tcgtgaggca caaggtgctc gacctcgtcg
      601 gcgacctcgc cctcgtcggc cgcccggtcg tcgggcacgt ggtggcgcac cgcgccgggc
      661 acgcgctcca cacggcgctt gcgaaggaga tccgccaggc gctcgccggc gagcacctcc
      721 gccgcgtcga gccggccccc gagtcggtcg ccctcgccgg ccgctgagga ttcgggagtt
      781 tccgcgcaaa atgcgcgggt gcgtccgagg cccctcgtcg tcgcagccgc gctgatcgcg
      841 ctcgccgccg ccttcccggc cccgggagcc gatccggatc ggcttcggat cccccaggtc
      901 gagacgactt tccagggccg cgacgtggcc gtggcggccg ggctgtcgcg gccgctcccg
      961 gaggacgtgc ggaagaggct cgcctcgggc ctcccgacga cggtgacgtg gcaggtgcgc
     1021 ctcttcgtct cgcggcggaa gtggtgggac gacccgctcg acgagcggcg ctacgcggtg
     1081 acggcgacct accgcccggt cggcggcgac ttcgccgtgg agcggaggct ggacgggcgg
     1141 ctcctcgaga cggtcgtcct cccgtcgctc gcggaggcgg aagccgcgct gtcggccctg
     1201 gccggcctcc cctgcttccg catggggccc gccctcgcgg ggccgacgct cgcggtgcgc
     1261 gtccggtgcg tctacgggtc gcgcctcgcg ctcggcttcc tcccgacggc ggtcggcacg
     1321 agctggcgga agtcgcccgt cttccgctgg cgggagccgg gaggcacgcc gtgaaggcgg
     1381 cgtgggcgcg cgacaaccgc gtcctgatcg ggctcggcct cctcctcgga gccacgttcg
     1441 ccgggatcta cgccctcgtc ctccgctcgc gggcgctcgc cgcgccggcc gcgacgaacc
     1501 gtgtcctcct cttcgtcctc acctacatcg tcgtcgtcct gatcctcgcg ctcctcctcg
     1561 tcatcgggcg gagcgtcttt cggctcctgc tcgacacgcg ccgcggcgtc ttcggctcgc
     1621 ggttccaggt ccggatcgtc ctgacgaacg tggggctcgc gctcctcccg atcgcgctcc
     1681 tgatcctgcc gacgacgggg ctgctgcagc ggagcgtcga cgtctggttc gcgccgccgg
     1741 tgacgcagac gatcgagtcg gggcgggaag tggtggagat cgtgcggcgg cgcgcggccg
     1801 agcgcgagct ccgggtggcc gcgcggctcg cggccacgct ccccgaggcg cggggcgaca
     1861 cggcgcgcct cgccctcctc gcggccgcgc gcgaggagtc gggcctcgac tacgtcgagc
     1921 tgcgccccgc ggggaacggg gcgcgcccct ccgtcctcgc ggtgagcgcg ccgcgctggc
     1981 cgctgcgcga cgtgccggag ccgtcgtcgg agtgggtctc cgccgcccgc gcgcgcggct
     2041 cggtccgccg cgtcgactcg gtggacggcg gcggcctcgt cggccggacg gtcatcgcgg
     2101 accgcgccgg gctcctcgtc ctcggcacct acgagccccc ggcggaggcg gcgccgctcc
     2161 gggcgctctc gcgttcgacg tcggcgttcg cgcagctgga ggccgagcgg ggctcgatcc
     2221 aggcggtgca ggtgctcctc ttcctcctcc tcgcgctcct cgtcctcctg gcggcggtct
     2281 gggtcggcct cgtcctggcg cgccgcgtca cgcggcccgt cgccgcgctc gcgcgctcgg
     2341 tccggagggt cggcgccggc gacttcaccg cccgcgtgga ggtggaggga gccgacgaga
     2401 tcgcgacgct cgggagcgcg ttcaacgcga tgaccgaaga gctccgcggg agccgcgagc
     2461 agctcgtctc ggcgaacgcc gccctccggt cggcctacgc gcggatggac gaggagcggc
     2521 tgcgcatccg gacgatcctc gcgcacctcg acgtcggggt cctcgccttc gggccggccc
     2581 cgggcgagga cggaggcgcc tcccggctcg gcgggatgaa cgacgccgcg atgcggatgc
     2641 tccgaacgcc ggacgcgccg gccgggacga cgctcgcgga gctcctcccc ggggactggg
     2701 ccgctccgct tcaggagctc ctcgcggggg ccttccgcgg gaccggctcg cgcgagggga
     2761 ctctgtcgct ctcgccgccc ggcgcgcgcg agccgatggt cctcgaggtg cacgtgaccg
     2821 gcgtcgcagg cgagggtccg gagcggacgg cgtgggtcgt gacgctcgag gacacgaccg
     2881 cgctcgtccg ggccgagcgg gccgccgcct gggaggaggc cgcccggcgg atggcccacg
     2941 agatcaagaa cccgctcacg ccggtgaggc tcgccgcgga gaggatccgg aggaaggccc
     3001 gcggctcggc cggctgcgac ccggagctcg cgaaggtcgt cgaggagggc gccgacacga
     3061 tcgtgcagga ggtgcgctcg ctcgcggcgc tcgtcgacgc gttccaccgc ttcgcgcggc
     3121 ttccggagac gcggctcggc gagctggacc tcggcgcggt cgtcgcgcag gtcgtgaagc
     3181 tgtacgaggg gacgaaggcc ggcgtccgcg tcgccgccga gccggctccg gacctcccgg
     3241 cggtccgcgg ggacgccgag caggtgaagc gcgccctcgt gaacctcgtc gacaacgccg
     3301 tcgccgcgac gccgccgggc ggcgccgtgt cggtccgagc ctcggtggac gggggcctcg
     3361 cccgcgtcgt cgtcgccgac gacggccccg gcatcccgcc ggaggagcgc gacctcgtct
     3421 tcgagccgac gttctcgacg aaggcgaacg ggtacgggct cgggctctcg atcacgtcgc
     3481 ggatcgcggc ggagcaccgc gggcgcgtga tcctggagga gaatgccccc cggggatgcc
     3541 gattcgtctt cgagtggccg gccgcctgac gtctcgcccg cggaggaggc ggtgagcgcc
     3601 cccgggccgc ggggagcgac ggtactcgtc tgcgacgacg ccgccgggat ccggaagacg
     3661 ctcgcgcaga tcctgggcga cgagggatac cgcgtcgaga cgttcgagga cggcgggtcg
     3721 gtcctcggac gcctcgggga gccgaagctg ccgcggccgg ccgcgctctt cctcgacgtc
     3781 tggctgccgg acgtcgacgg gctctccgtc ctcgaccagc tccgcgcgcg cgggcacgac
     3841 ctgccggtgg tgatgatctc cgggcacggc acggtggaga cggcggtgca ggcggtcaag
     3901 cgcggcgccg acgacttcct cgagaagccg ctcgcgctcg agcgggtgct gctgacgctc
     3961 gagaaggcgc tcgagaggag ccggctcgcc cgcgagcgcg acgtcctcgc ccgagaggtg
     4021 gcccggcagc gggaacggct cgaggaggag gagacgctcc tcggagaggg acccgcgatg
     4081 acccgcctgc gcgaggagct ccggcgcgcc ggggcgtccg acgcccgggt gctgatctcg
     4141 ggcgagagcg gcgtcggcaa ggaggtcgcg gcacggacgc tccaccgcct ctcgccgcgc
     4201 gcgggagggc cgttcgtgga cgtgaacggc gccgcgatcc cggaggacct gatcgagagc
     4261 gagctcttcg ggcacgtgaa gggctcgttc accggagcga ccgaggaccg caaggggaag
     4321 tgggaggccg ccgacggcgg gacgctcttc ctcgacgaga tcggcgacat gtcgccgcgg
     4381 acgcaggcga agatcctccg cgcgatccag gacgggaaga tctcgcgcgt cggcgcgacg
     4441 cggacgatcg agaccgacgt gcggatcgtc gccgccacga accgcgacct gccgaagatg
     4501 atcgcggccg ggacgttccg ggaggacctc tacttccgcc tcgccgtcgt cccgatccgc
     4561 gtcccgacgc tcgccgagcg cccggaggac atcccggccc tcgtcgccca cttcgcgggg
     4621 aagctggcgc ggcgacgcgg ccggcggccg ccgaccttct cggccgaggc cctcgaggag
     4681 ctgaagcggc gcccctggcc ggggaacgtc cgcgagctgc agaacgtcgt cgagcgggcg
     4741 ctcctcctcg cggacggcgc cgtcatcggc gccgacgacc tccccggcga cgtcctgcgc
     4801 ctgccgcgga cgggaggcga cgcgggagac ggcctgatgc cgggccagac gctcgccgac
     4861 gcgcgcgacg cgttcgagcg
//
LOCUS       DAOTEZ010000337         4860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_12635_length_4860_cov_5.601873, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000337 DAOTEZ010000000
VERSION     DAOTEZ010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12635_length_4860_cov_5.601873"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="P5164_15200"
     CDS             complement(<1..1251)
                     /locus_tag="P5164_15200"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRY45285.1"
                     /translation="MNVLLRPPEFDHYSVSRVLGEGGMGIVFLAEDRRTHLPVAVKVM
                     SRSLTDPELQARFMKENQILASLNHRNIVRCYEITRSREGLPTIVMEYVKAVDFGAFE
                     GRPFPELIPLMVQAAMGLAYLKERNILHRDLSPNNIMVTLQDERRLVKILDFGVAKVL
                     QEGSGGELTQTGEFLGKLAYASHELLTFGQVDFRSDIYSLGVIFFRLLTKRRPINVQN
                     SRNYLEWVMAQENRGPIDFTAPEGNPAIPEPLQALVLKMLAKKSEDRPQGYEEIIDAL
                     VAAQSEAEKSGLVPDPETVSTLPSPEARSSISGSAGTPASPAGAGSGAPLFDDGAFPF
                     TEAETKAPSSASRLAASDFPARAPAPKAAPASQMPDWLEQADYNEQMQVISESKQGIS
                     RSPVVHPVFGGESRSRPIKRDTESI"
     gene            complement(1375..2289)
                     /locus_tag="P5164_15205"
     CDS             complement(1375..2289)
                     /locus_tag="P5164_15205"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRY45286.1"
                     /translation="MRVFVTGATGHIGSAVAAAFARAGHETIGLVRDLRKTAPLEAAE
                     VVPVLGTMEEPDAWREVAGRCSVLVHCAVDSSDRSWDLHRAALGALRDAARDRTDRVL
                     LATSGVWVYGDTGEAAVDESTPLDPPTFSAPRPAVDAAVLSWNGAGLRTLVIRPGCVY
                     GGGGSLTAAWFESAARDGAARIVGDGENRWAMVHRDDLADLYVRAAESGLGGEVLNAT
                     DRSRATVLDCARAASRAAGAEGRVEHVPVEQAVAAMGPFATGLALNQHVASWKAVRRL
                     GWTPRHAGFVDEAPRLFAAWRASRPEGA"
     gene            complement(2336..3337)
                     /locus_tag="P5164_15210"
     CDS             complement(2336..3337)
                     /locus_tag="P5164_15210"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRY45287.1"
                     /translation="MKYTQLGRTGLVVSRLALGCMSYGDPRWRPWVLSEEDARPFFRQ
                     ALEAGINLFDTADMYSLGVSEEVTGRLLGEMGRRDELVIATKVHFPMGPGPNRSGLSR
                     KHVVQACEASLRRLGVETIDLYQIHRFDPATPIAETLEALDLLVRQGKVRYVGASSGE
                     AFRMAKALATADLRGFPRFVSMQNHYNLLYREEEREMLPLCAEEGLGVLPWSPLARGL
                     LAGTRATPADREATPRAATDDYARQLYDHPTDADVIGATRAVASARGVPMARVALAWL
                     LARPAVTAPIVGATKLAHLDDALAALDLELTPEETAALEEPYRPHAVRGFAFAPPAR"
     gene            complement(3534..4718)
                     /locus_tag="P5164_15215"
     CDS             complement(3534..4718)
                     /locus_tag="P5164_15215"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY45288.1"
                     /translation="MKRDFAISGTTLVLAAALGGALLTQAACKKSGPDIKIGVIAELS
                     GDISEIGDACKKAAELAVSEINEAGGVEVAGTKHLLRLVIEDSQGAAQPAAEAAMRLI
                     DQEKVLAIVGPNASLGAVPAAQVAAEKKTLLISPWGTAPRVTRDDAGAPRQWVYRTCF
                     TDAFQGSALAKFAAAYMKATKAAVLYNPASEAPKSQAELMKKEFEGRGGQVVAFETYA
                     TGDKDFTAQWKKIAEAAPDVVFLPAYYGEIPEQIRQARAAGVKAPFLGSDTWTNSELL
                     KSAAEVDGAFFCAHYNPDAMEDKVGIFRGAYENRYNKEVPDDGAALTYDTLYLLKQAL
                     ETATGDDREAVREAFSKISSFDGVTGRIVFKGGAPDPVKSVVIKQFKAGKISFVTNID
                     PD"
BASE COUNT          656 a         1658 c         1819 g          727 t
ORIGIN      
        1 gatcgactcg gtgtcgcgct tgatcggcct cgacctcgac tccccgccga acacggggtg
       61 gacgaccggc gagcgggaga tcccctgctt gctctcgctg atcacctgca tctgctcgtt
      121 gtagtcggcc tgctcgagcc agtcgggcat ctgcgaggcg ggggcggcct tcggggccgg
      181 agcccgagcg gggaaatccg acgccgcgag ccgcgaggcg gaggacgggg ccttcgtctc
      241 ggcttcggtg aaggggaacg cgccgtcgtc gaagagcggc gccccggagc cggccccggc
      301 tggagacgcg ggagtcccgg ccgagccgga gatcgagctg cgcgcctcgg gcgacgggag
      361 ggtcgagacc gtctcggggt ccgggacgag gccggacttc tccgcctcgg actgggcggc
      421 gacgagggcg tcgatgatct cctcgtaccc ctgcggccgg tcctcggact tcttcgcgag
      481 catcttcagg acgagcgcct ggagcggctc cgggatcgcc gggttcccct cgggggcggt
      541 gaagtcgatc ggcccgcggt tctcctgcgc catcacccac tcgaggtagt tcctcgagtt
      601 ctggacgttg atcgggcggc gcttcgtgag gaggcggaag aagatgacgc cgagcgagta
      661 gatgtcgctc cggaagtcga cctgcccgaa cgtcaggagc tcgtgcgagg cgtaggcgag
      721 ctttccgagg aactccccgg tctgagtcag ctccccgccg ctcccctcct ggaggacctt
      781 cgcgacgccg aagtcgagga tcttcacgag gcgccgctcg tcctggaggg tgaccatgat
      841 gttgttcggc gagaggtcgc gatggaggat gttccgttcc ttgaggtacg cgagccccat
      901 cgcggcctgg accatgaggg gaatgagctc ggggaacggc cggccctcga aggcgccgaa
      961 gtcgaccgcc ttcacgtact ccatgacgat cgtcgggagc ccctcgcgcg accgggtgat
     1021 ctcgtagcac cggacgatgt tccggtggtt cagcgaggcc aggatctggt tctccttcat
     1081 gaaccgcgcc tggagctcgg ggtcggtcag gctccgcgac atcaccttga cggccaccgg
     1141 caggtgcgtc cggcggtcct ccgcgaggaa gacgatcccc atcccaccct cgcccaggac
     1201 gcgagagacg gagtagtggt cgaactcggg aggtctgagg aggacgttca acgtgagacc
     1261 gtttcccgga ttgtacggcc cattccggaa ctcagatcgc ggcgccggcg cggaggcgcg
     1321 cggcgtcctc gcccgtgagg tccggccagt cgatcgagcc gaccgcggag gcgctcacgc
     1381 accctccggc cgggaggccc gccaggccgc gaagaggcgg ggcgcctcgt cgacgaagcc
     1441 ggcgtggcgg ggggtccacc cgagccgccg cacggccttc cacgacgcca cgtgctggtt
     1501 cagggcgagg ccggtcgcga acgggcccat cgcggcgacc gcctgctcga ccggcacgtg
     1561 ctcgacgcgc ccctccgccc ccgcggcgcg gctcgccgcg cgggcgcagt cgaggacggt
     1621 ggcccggctc cgatccgtcg cgttcagcac ctcgcctccg aggcccgact ccgcggcccg
     1681 gacgtagagg tccgcgaggt cgtcgcggtg gaccatcgcc cagcggttct cgccgtctcc
     1741 cacgatccgg gcggcgccgt cccgcgcggc gctctcgaac cacgccgcgg tgaggctccc
     1801 gccgcccccg tacacgcacc cgggccggat cacgagcgtc cgcaggccgg cgccgttcca
     1861 ggagaggacg gcggcgtcga cggccggacg gggcgcggag aacgtcggcg ggtcgagcgg
     1921 ggtcgactcg tcgaccgcgg cctcccccgt gtcgccgtag acccagacgc cgctcgtggc
     1981 gaggaggacg cggtcggtgc gatcgcgggc ggcgtcgcgc agggctccga gggcggcgcg
     2041 gtggaggtcc cacgagcggt ccgacgagtc gacggcgcag tggacgagga cggagcagcg
     2101 tccggcgacc tcccgccagg cgtcgggctc ctccatcgtc ccgaggaccg gcacgacctc
     2161 cgcggcctcg aggggcgcgg tcttccgcag gtcccgtacg agaccgatgg tctcgtgccc
     2221 ggcgcgggcg aaggcggccg cgacggccga gccgatgtgt cctgtggctc cggtgacgaa
     2281 gactctcatg agggacctcc tccgggacgg gtccggatga taggccgcgg acgcctcagc
     2341 gggcgggcgg cgcgaacgcg aagccgcgga cggcgtgggg cctgtagggc tcctcgagcg
     2401 cggcggtctc ctccggcgtg agctcgaggt cgagcgcggc gagcgcgtcg tcgaggtgcg
     2461 cgagcttcgt cgcgccgacg atcggcgcgg tgacggcggg acgcgcgagg agccaggcga
     2521 gcgcgacccg ggccatcggc acgccccggg ccgaggcgac cgcgcgcgtc gcgccgatca
     2581 cgtcggcgtc cgtggggtgg tcgtagagct gccgggcata gtcgtccgtg gcggcgcggg
     2641 gcgtcgcctc gcggtctgcc ggcgtcgccc gcgtcccggc gaggagaccc cgggcgagcg
     2701 gcgaccacgg gaggaccccg agcccctcct cggcgcagag cgggagcatc tcccgttctt
     2761 cctcgcggta gaggaggttg tagtggttct gcatcgagac gaagcgcggg aagccgcgga
     2821 gatccgcagt cgcgagggcc ttcgccatcc ggaaggcctc acccgagctc gccccgacgt
     2881 agcggacctt cccctggcgg acgaggaggt cgagcgcctc gagcgtctcg gcgatcggcg
     2941 tcgcggggtc gaagcggtgg atctggtaga gatcgatcgt ttcgacgccg aggcggcgga
     3001 ggctcgcctc gcacgcctgg acgacgtgct tgcgtgagag cccggacctg ttcgggccgg
     3061 ggcccatcgg gaagtggacc ttcgtcgcga tcacgagctc gtcgcggcgt cccatctcgc
     3121 cgaggagacg acccgtgacc tcttcgctca ccccgagcga gtacatgtcg gccgtgtcga
     3181 agaggttgat ccccgcctcg agggcctgcc ggaagaaggg ccgcgcgtcc tcctccgaaa
     3241 ggacccacgg gcgccagcgc gggtctccgt agctcatgca gccgagggcg aggcgggaga
     3301 cgacgaggcc ggtgcggccg agctgggtgt acttcatggc gcgtccgtcc cgacgatcct
     3361 cgcacgccgc cggccattcg gtcccggtcg cccgcggaac gggggaaagc acgaagggcc
     3421 cgcgcgagcg ggcccttcgg ggaatgccgg gaagccgtta cgttcagtcg gggtcgatac
     3481 ggggaagggc cggaggcccg ggtggggctg aacgaggccg agtgtggcct cgttcagtcg
     3541 gggtcgatgt tggtcacgaa ggagatcttc cccgccttga actgcttgat cacgacgctc
     3601 ttcaccgggt cgggggcgcc tcccttgaag acgatccggc ccgtcacgcc gtcgaagctc
     3661 gagatcttcg agaaggcctc gcgcaccgcc tcgcggtcgt cgccggtggc ggtctcgagc
     3721 gcctgcttga ggaggtagag cgtgtcgtac gtcagggcgg cgccgtcgtc cgggacctcc
     3781 ttgttgtacc ggttctcgta ggccccgcgg aagatcccga ccttgtcctc catggcgtcg
     3841 gggttgtagt gggcgcagaa gaacgcgccg tccacctcag cggcgctctt gaggagctcg
     3901 gagttcgtcc aggtgtcgct cccgaggaag ggggccttca cgcccgccgc ccgggcctgc
     3961 cggatctgct cggggatctc cccgtagtag gccggaagga agacgacgtc gggcgccgcc
     4021 tcggcgatct tcttccactg ggccgtgaag tccttgtcgc cggtcgcgta ggtctcgaag
     4081 gcgacgacct ggccgccgcg cccctcgaac tccttcttca tcagctcggc ctggctcttc
     4141 ggggcctcgg acgccgggtt gtagaggacc gcggccttcg tcgccttcat gtaggccgcc
     4201 gcgaacttgg cgagcgccga tccctggaag gcgtcggtga agcaggtccg gtagacccac
     4261 tgccgcggcg ccccggcgtc gtcccgcgtg acacgggggg cggtccccca gggcgagatc
     4321 aggagcgtct tcttctcggc ggccacctgg gcggccggga ccgcgccgag gctcgcgttc
     4381 ggtccgacga tcgcgaggac cttctcctgg tcgatcaggc gcatcgccgc ctcggcggcc
     4441 ggctgggcgg ccccctggct gtcctcgatg acgaggcgga ggagatgctt cgtgccggcg
     4501 acctcgacgc cgccggcttc gttgatctcg gagacggcca gctccgcggc cttcttgcag
     4561 gcgtcgccga tctccgagat gtccccgctc agctcggcga tgacgccgat cttgatgtcg
     4621 ggtccgctct tcttgcaggc cgcctgggtg aggagggcgc cgccgagggc cgcggcgagc
     4681 acgagggtcg ttccggagat cgcaaagtcc cgcttcatag ccggtaagcg tatttccgga
     4741 cggggggcgc ggtcaaacgg tcttccattc tagaatccga ctttcgcccc ggggaggggc
     4801 ggcgcgcgct cgacggacgt ccggaaggag cctcgcggga ggtgcccgga gcggtcccgc
//
LOCUS       DAOTEZ010000338         4859 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_12639_length_4859_cov_6.532057, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000338 DAOTEZ010000000
VERSION     DAOTEZ010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12639_length_4859_cov_6.532057"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..748)
                     /gene="lexA"
                     /locus_tag="P5164_15220"
     CDS             complement(134..748)
                     /gene="lexA"
                     /locus_tag="P5164_15220"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HRY45289.1"
                     /translation="MANRPNLTERQQEIVAFIRDYRTRRGIAPTQREICEHFGYSSFG
                     TLQKHIRLLLDKGVLVRDWNQRRSLDVAEEAASAPALEVPLLGRIAAGSPIEVLPGDD
                     RLHVPESLTRKGENFVLLVSGDSMIDEGIRDGDWVVVHRRTRAENGEMVAALVSGEAT
                     LKRLYREAGGVVRLQPSNERLAPIRAREEDVQVQGIVVGLMRRY"
     gene            1073..1657
                     /locus_tag="P5164_15225"
     CDS             1073..1657
                     /locus_tag="P5164_15225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRY45290.1"
                     /translation="MTNSWADPFEAELGRLPEKLRRFFLSRGVRTEDEACDLVQETLL
                     RVLQRASRGEPLDSPEAFALGVAKNVFFEYCRRVQKANKHEGLSGVLELAGTEDPLSD
                     ALSSSRRLIVRKALELLPDRMKSLLVRRFVEEVPSRTIAREEGITTDAVDMRVYRAKR
                     ELRKKMDSTAARSAVRTQREEEARRGPDDEGGEA"
     gene            1654..2496
                     /locus_tag="P5164_15230"
     CDS             1654..2496
                     /locus_tag="P5164_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45291.1"
                     /translation="MSREPAVDRGRIRGYLLRRLAEDERQSFEESYFADDALLDRVEA
                     EEDLLVTDYVLGRLSEEDRRRFEQALLGTPYYRERVETTTRLRRRIARDQGVRNLGRG
                     AAPRPGGGLFPGRTGIVVAIGLLCVLLLAALVTALRLKQQLATLRAELSTSAPAPVTA
                     AGGGVVPSTPSVVLQPTEGAGVSVRRVERLAGSPLVLVFPRDLLPDDATSWRVVLREE
                     SGAVIWDSGDQTVPAAASSDLAVRLPPGVPAGGRSGVTLWSGGTPVFSALLQIAEPPA
                     AAPR"
     gene            complement(2651..3457)
                     /locus_tag="P5164_15235"
     CDS             complement(2651..3457)
                     /locus_tag="P5164_15235"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRY45292.1"
                     /translation="MSAVRSPRQEDDAMGNGGVSVTVDGRSATVTFHRPPDNVLDRET
                     VVALESRLREVARRRDVRLVALAARGEDFCNGTEILPPGDGRSDSLFEAWHSLVRFLL
                     TCEVPTCALVQGRALGAGAELALACDFVFAETTATFGFPEIHRGSFPPVATVLLERRT
                     GRTKAADLILCGAAMTAEAAERRGLINALVNPGDLIEALDTMRTRLETLSASSLRLAK
                     RALNQGCSGDLLAALSAVERTFSRELMKTEDAAEGAKASLEGRPPVWIDR"
     gene            complement(3512..3588)
                     /locus_tag="P5164_15240"
     tRNA            complement(3512..3588)
                     /locus_tag="P5164_15240"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3552..3554),aa:Pro,seq:cgg)
     gene            complement(3671..>4859)
                     /locus_tag="P5164_15245"
     CDS             complement(3671..>4859)
                     /locus_tag="P5164_15245"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRY45293.1"
                     /translation="VVPEGLSRDLEEERRLLTRWEETRRLLFPRGRPLAPGARLVQPE
                     LAETLARIGRDGPDALHRGEIAARIAAYVRAHGGVLAEGDLEGYAPVWRAPEEIRFGD
                     LVVHTMPLPSSAGLVLRSVLGQLEVARGTGPFRPDAAGYHLLLEAERRAYADRNRWLG
                     DADCVEVPLKELLAPGHLSRHGGSIDPGRATPSASVPGGLPREREETTHLSAATPDGF
                     AVSLTTTLNGSFGNGAIVPGTGVLLNNEMDDFAAAPGTPNLYGLVQGSANAVRAGARP
                     LSSMAPAIVERAGRPLLVVGSPGGSTIPTTVLQVLLRVAAGEDLASAVAAPRLHHQHT
                     PDVVFFERGREASAVLEGLRRRGHVPRERGPIGKVHAVVFAPDGRLVGAADPRGYGAS
                     AAP"
BASE COUNT          726 a         1760 c         1669 g          704 t
ORIGIN      
        1 gtatccccaa ggggtgatcg gaatatgggg catgccgccg gacccgtcaa gtgagcgcca
       61 gcacaacccg ggcgcgtgca ggcgcgcgcg ctcggccgcc gaaacggcca catcccgcgc
      121 gtcccggacc agatcagtac cggcgcatca gtccgacgac gattccctgg acctggacgt
      181 cctcttcccg cgcacgaatc ggggccaggc gctcgttcga gggctgcagc cgaacgacgc
      241 cgcccgcctc acggtagagc cgcttgagcg tcgcttcccc gctcacgagc gcggcgacca
      301 tctccccgtt ctccgccctc gtcctccgat ggacgacgac ccagtcgccg tcccgaatcc
      361 cttcgtcgat catcgagtcg ccgctcacga ggaggacgaa gttctcccct ttccgcgtga
      421 gcgactccgg cacgtgaaga cgatcgtcgc cggggaggac ctcgatcggg ctaccggccg
      481 cgatccggcc gagaaggggg acctccaggg cgggcgccga cgcggcctcc tcggccacgt
      541 cgagagagcg gcgctggttc cagtcgcgta cgagcactcc cttatcgagg agcagccgaa
      601 tgtgcttctg aagggtgccg aaggaggagt agccgaagtg ctcgcagatt tcgcgctggg
      661 tcggcgcgat tccccgccgc gtccgatagt cccggatgaa ggcgacgatc tcctgttgtc
      721 tctccgtaag attcggacga tttgccataa tttattcggc tcccatacca tttctccata
      781 tttcgttcgg aaacgatggg tcgcgctcaa cgaaagtatg gcgcaaatcg cgcgcaagtc
      841 aagagaatcg ccatctccag gatgccagga cgtctctcgc tctcgcgcca ggacttcgct
      901 tcgccccccg ccgccttcga gtgcagcgga cacctgccta attatgttcg gcgaaattgt
      961 gagttctccg aaatgtattc gggaaatttc cacagtgacc gctctcgaca tagaatcccc
     1021 tcgtggtgac gcacggcgcg gcccgccgtt tgcttcgatg atgagaagga cgatgacgaa
     1081 ttcctgggcg gaccccttcg aggccgagct cgggcgactc ccggagaagc tgcggaggtt
     1141 cttcctctcc cgcggcgttc gtacggagga cgaggcctgc gacctcgtcc aggagaccct
     1201 gctccgggtc cttcagaggg ccagccgcgg cgagccgctc gattctcccg aggcgttcgc
     1261 gctcggcgtg gccaagaacg tcttcttcga gtattgccgt cgggtccaga aggcgaacaa
     1321 gcacgaaggc ctctccggcg tcctcgagct cgcaggcacc gaggacccgc tctccgacgc
     1381 gctttcgagc agccggcgcc tcatcgtccg gaaggcgctc gagctcctcc ccgaccggat
     1441 gaagagcctg ctcgtccggc gcttcgtcga ggaggtcccc tcgcggacga tcgcccgcga
     1501 ggaggggatc acgaccgacg ccgtcgacat gcgcgtctac cgcgccaagc gcgagcttcg
     1561 aaagaagatg gactccaccg ccgcgcgatc cgccgtgcgg acccagcgcg aggaggaggc
     1621 ccggcgcggt ccggacgacg aagggggcga ggcgtgagcc gcgagcctgc cgtggatcgc
     1681 ggccggatcc gcggctacct cctcaggcgc ctcgcggaag acgagcgcca gagcttcgaa
     1741 gagtcgtact tcgccgacga cgccctcctc gaccgcgtcg aggccgagga ggacctcctc
     1801 gtcaccgact acgtcctcgg ccgcctctcc gaggaggacc gccgcaggtt cgagcaggct
     1861 ctgctcggga ccccctacta ccgcgagcgc gtcgagacga cgacgcggct ccgacggcgg
     1921 atcgcgcggg accagggagt ccggaacctc ggccgcggcg cggcgccccg gcccggcggc
     1981 ggcttgttcc ccggccgcac gggaatcgtg gtcgcgatcg gcctcctctg cgtgctgctc
     2041 ctcgcggcgc tcgtgacggc gctccggctg aagcagcagc tcgcgacgct ccgggccgag
     2101 ctctcgacct ccgcgcccgc cccggtgacc gcggccggcg gcggcgtcgt gccgtcgacc
     2161 ccctcggtcg tcctgcagcc aaccgagggt gccggggtct ccgttcgccg cgtggagcga
     2221 ctggcggggt cgccgctcgt cctcgtcttc ccgcgcgacc tccttccgga cgacgcgacg
     2281 agctggagag tggtcctgcg cgaggagtcg ggggccgtca tctgggacag cggggaccag
     2341 accgttccgg ccgcggcgtc ttccgacctc gcggtccggc tgccgcccgg cgtcccggcg
     2401 ggaggtcgaa gcggcgtgac gctctggtcc ggcgggacgc ccgtcttctc ggccctcctc
     2461 cagatcgcag agccccctgc cgccgcgccg cgttaggtct cgcggacttc cgtcactacg
     2521 gggcgaaggg tgagttcgat tccttttccg ctctctcctc cctgaccccg gaaggcgccg
     2581 cgcccctccg cggcgccgga ccctccccgc gccccgcccg ggtcctcccc gcggcggggc
     2641 gttcctcttc ttaccggtcg atccagaccg gcgggcgccc ctcgagggac gccttcgccc
     2701 cctccgccgc gtcctccgtc ttcatcagct cgcgggagaa cgtcctctcg accgccgaga
     2761 gcgccgcgag gaggtcgccc gagcagccct gattgagggc tcgcttcgcg agccggagcg
     2821 acgaggccga gagcgtttcc agcctcgtcc tcatcgtgtc gagggcctcg atcaggtcgc
     2881 ccggattcac gagggcgttg atcaggcccc gccgctcggc cgcttcggcc gtcatcgcgg
     2941 cgccgcagag gatgaggtcc gcggccttcg tccttccggt ccggcgctcg aggaggacgg
     3001 tcgcgacggg agggaaagag ccgcgatgga tctccgggaa gccgaacgtc gccgtcgtct
     3061 cggcgaagac gaagtcgcac gccagcgcga gctcggcgcc cgcgccgagg gcgcgcccct
     3121 gcacgagggc gcaggtcggg acctcgcagg tgaggagaaa gcgtacgagg gaatgccagg
     3181 cctcgaagag ggagtcggag cgtccgtcgc cgggcggcag gatctccgtc ccattgcaga
     3241 agtcctctcc ccgcgcggcg agcgccacga gccggacgtc ccgccggcgc gcgacctccc
     3301 ggaggcgaga ttcgagggcg acgacggtct ccctgtcgag gacgttgtcg ggaggcctgt
     3361 ggaacgtgac cgtcgcggac ctgccgtcga ccgtgaccga gacgccgccg ttgcccatcg
     3421 cgtcgtcctc ctgccgcggc gaccggaccg cgctcacaaa gcctaacgga aaaagacccc
     3481 gctcgggagg cggggtcgtc gatcgcgatg atggtcgggg cgagaggatt cgaacctccg
     3541 accccacggt cccgaaccgt gtgcgctacc aggctgcgct acgccccgac ggaagcgcgg
     3601 acgcggaaga tagcacgagc cctcgcggaa aggaagacgg cgaagtgtcc cgcggcgagg
     3661 gccgcgcgcc tcagggcgcc gcgctcgcgc cgtagccccg cgggtccgcc gcgccgacga
     3721 gccggccgtc cggcgcgaag acgacggcgt ggaccttgcc aatcggacct cgctccctcg
     3781 ggacatgccc tcgccgacgg agcccctcga ggaccgcgga agcctcgcgt ccccgttcga
     3841 agaagacgac gtccggcgtg tgctggtggt ggagccgcgg agccgcgacg gccgaggcga
     3901 ggtcctcgcc cgcggcgacc cggagaagca cctggagcac cgtcgtcgga atcgtcgacc
     3961 ctccgggcga accgacgacg aggagcggcc gacccgccct ctcgacgatc gccggagcca
     4021 tcgaggatag aggccgcgca ccggcccgaa ccgcgttcgc gctcccctgg acgagaccgt
     4081 agaggttcgg cgtccccggc gccgccgcga agtcgtccat ctcgttgttc agcagcaccc
     4141 cggttcccgg cacgatcgcc ccgttcccga aggagccgtt cagcgtcgtc gtgagcgaga
     4201 ccgcgaagcc gtccggtgtc gcggcggaga gatgggtcgt ctcctcccgc tcgcgcggga
     4261 ggccgcccgg gacggatgcc gagggcgtcg cccggccggg gtcgatcgaa ccaccgtgcc
     4321 gggagaggtg ccccggcgca aggagctcct tcagcgggac ctcgacgcag tcggcatctc
     4381 cgagccaacg gttccggtcg gcgtaggctc gcctctccgc ctccagcagc agatggtatc
     4441 cggccgcatc cgggcggaac gggcccgttc cgcgcgccac ctcgagctgg ccgaggaccg
     4501 atcgaaggac gaggccggcg gaggatggaa ggggcatggt gtggacgacg aggtctccga
     4561 aacggatctc ctccggcgca cgccagaccg gtgcgtagcc ctcgaggtcc ccttccgcga
     4621 ggacgcctcc gtgcgcccgg acgtaggccg cgatccgtgc cgcgatctcg ccgcggtgga
     4681 gcgcgtcggg cccgtcgcgc ccgatccgcg cgagagtctc ggcgagctcc ggctgcacca
     4741 ggcgcgctcc cggggcgaga ggtcggcccc gcggaaaaag gagccggcgc gtctcttccc
     4801 agcgcgtgag gagtctgcgc tcttcctcga gatcgcgaga gagcccctcg ggaacgacg
//
LOCUS       DAOTEZ010000339         4849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_12681_length_4849_cov_5.721944, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000339 DAOTEZ010000000
VERSION     DAOTEZ010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12681_length_4849_cov_5.721944"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..100)
                     /locus_tag="P5164_15250"
     tRNA            complement(26..100)
                     /locus_tag="P5164_15250"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66..68),aa:Gln,seq:ttg)
     gene            complement(116..976)
                     /locus_tag="P5164_15255"
     CDS             complement(116..976)
                     /locus_tag="P5164_15255"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRY45294.1"
                     /translation="MRLVAEAFAKVNRSLLVLGRRPDGFHELDTVFQAVGLTDRLAFE
                     ESDALSLRVDDPSVPAGPENLVLRAARLLAEEAGVKARAAIGLEKRIPSGGGLGGGSS
                     DAAVTLLGLSALWGLDAPLETLAEIGARLGSDVPFFLFGGSARGTGRGERIAPLPDLP
                     PKGVVLVMPPFPAATPEVFRRLGAPEWDGRRDDAGSGLPDRNDLEPAAEALFPPLRVV
                     REALAGVGAEQARLSGSGSTVFGLFPDLAAAAEAARRLDGLPVGSAIRVVPTWTREEC
                     RFRSGPRRSE"
     gene            complement(973..1611)
                     /locus_tag="P5164_15260"
     CDS             complement(973..1611)
                     /locus_tag="P5164_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45295.1"
                     /translation="MRRTAVAAALLVLGACRSAGPAGVPAGPEETARALASVSAAREA
                     AWRPRRFQALFRGELSPRVGVAVRGYLALQWDGAALSWKVSAPLAGAGREGVLRRAGG
                     GAEGLLPGRLSADDVLAAIFGVPEEAPSAEGASLLRSGRLVLPLPSGEGRAVLVSPAG
                     AITGLLLPDGVRVELTPGAGLPRRIAIRGPEGSAELTLESFAAVPEAGGANG"
     gene            complement(1608..3557)
                     /locus_tag="P5164_15265"
     CDS             complement(1608..3557)
                     /locus_tag="P5164_15265"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY45296.1"
                     /translation="MRFRPAAAALALAILAPPAAAAPPARSEALSPDESARRDAVERF
                     LRARLFAAEGEFQEALKEFRRAVELDPADGHLRREYAEALRDFGILPEAEQQARKAVE
                     LVPGSAAAHRVLGQVLLAAARDRNGIEGAIAPLKTANDLQPMEPAGALALGQAYLRLE
                     RYEDAAAVLSRVQQNVRGPLLPLLYGEALEKSGRPEEAEEPYLGILRMDAQNAAASFG
                     LLRVYQATRKFDKALPILAELVKRQPGNLGLRAQYGLSLLRARRLSEAEEAFQEVLKV
                     DPDHRDALRHYAALLSEKLETARAEELLKKLQGLEPDDADVAFRRALNFLEARRLDEA
                     ETVLRDLRSQLVARKGPAGGIVSVDGQLGYVAYLRKDWAAAREAVRPHLLEEDGSVNM
                     QALNLLTLVARDAKEPAQGLQACREAFAKAPKDLDVRGLLAEFLLRSEKEKERAEGEE
                     MVAALSREGREGALAAADVWQRLERYGRAADAASAGLAAFPDDPDLLFRQAASLERDK
                     KVPESIAAFEKLISLKPDHGAALNYLGYMFADRNENLDRALELVTRAVALEPSNAAYL
                     DSLGWIQFRLGNLDEAEKHLLTAKRLSPDDPTIEDHLGDLEEKRGDLAKARERWSRAL
                     ALGPEDGGAAIRAKLERTAAAAKQP"
     gene            complement(3625..>4849)
                     /locus_tag="P5164_15270"
     CDS             complement(3625..>4849)
                     /locus_tag="P5164_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRY45297.1"
                     /translation="EGIDLGTVTTSMTINSTAPIALAMYIAVAEKQGTPWSKVGGTLQ
                     NDILKEYIAQKEFIFPPRPSMRLVTDQFSFCAKHVPKWNTVSISGYHIREAGSTALQE
                     LAFTLRDGMEYVKYGVDAGLSVDEFAPRLSFFFNAHNDFFEEIAKFRAARRIWAKVMR
                     DRYGAKSPESWKLRFHTQTAGVSLTAQQPYNNVARVAIQALAAVLGGTQSLHTNALDE
                     TLALPTEFAAKVAVRTQQILMHETGVAHTIDPLGGAYFVEEATNRMEEGAFDYFRKID
                     AYGGMVEAIEAGFPQKEIMDAAYRFQRAFDVGDKVMVGVNAFTEEVPEDEVPTLVISE
                     QTAAEQVAQLREVRRTRDGKAVVSTLHALREACRNGANVMPPLVEAVKAYATVGEISD
                     VMREVFETWQEPAIF"
BASE COUNT          674 a         1768 c         1722 g          685 t
ORIGIN      
        1 cctcagcgtc atggctctct catcctggct ggggagggag gattcgaacc tccgaatggc
       61 ggttccaaag accgctgact taccacttgt cgactcccca aagccgggcc ggatcttact
      121 ctgaccgccg cggcccgctc cggaagcggc attcctcgcg ggtccacgtc gggacgacgc
      181 ggatcgcgga gccgacgggc aggccgtcga gcctccgcgc ggcctccgcg gccgccgcga
      241 ggtccgggaa gaggccgaac acggtcgacc cgctccccga gagccgggcc tgctcggccc
      301 cgacccccgc gagcgcctcg cgcacgaccc ggagcggggg gaagagcgct tccgcggccg
      361 gctcgaggtc gttccggtcg ggcagtccgc taccggcatc gtcccggcga ccgtcccact
      421 cgggcgcccc gaggcgacgg aagacctcgg gtgtcgccgc ggggaacggc ggcatcacga
      481 ggacgacccc tttcggcggc aggtcgggaa gcggcgcgat acgctccccc cggccggtcc
      541 cgcgggcgct ccctccgaag aggaagaacg ggacgtcgga gccgaggcgg gcgccgatct
      601 ccgcgagcgt ctcgagcggg gcgtcgaggc cccagagcgc cgagaggccg agaagcgtca
      661 cggccgcgtc cgagctgccg cctccgagcc cgccgccgga agggatcctc ttctccaggc
      721 cgatcgccgc gcgcgccttc acacccgcct cctccgcgag gaggcgggcg gctcgcagga
      781 cgaggttctc cgggccggcg gggaccgagg ggtcgtccac ccggagcgac agcgcgtcgc
      841 tctcctcgaa ggcgagccgg tccgtcagcc cgaccgcctg gaacaccgtg tcgagctcgt
      901 ggaagccgtc gggccgcctc ccgaggacga ggagggagcg attcaccttc gcgaacgcct
      961 cggcgacgag cctcatccgt tcgctcctcc ggcctccggg acggcggcga agctctcgag
     1021 cgtcagctcc gcgctccctt cggggccccg gatcgcgatg cggcgcggga ggccggcccc
     1081 gggggtcagc tccaccctta cgccgtcggg cagtagcagg ccggtgatcg ctcccgcggg
     1141 tgagacgagg accgcccgcc cctcgcccga agggagcggc aggacgagac gtccgctccg
     1201 aagcagcgag gcgccctcgg cggacggcgc ctcctccggg accccgaaga tcgcggcgag
     1261 gacgtcgtct gcggagaggc ggcccggcag gagcccttcg gcgcctcccc ccgccctgcg
     1321 gagcactccc tcccgtcccg cgccggcgag cggcgccgac accttccagg agagcgcggc
     1381 cccgtcccac tgcagggcga ggtagccgcg gacggcgacg cccaccctgg gtgagagctc
     1441 gccgcgaaag agcgcctgaa accggcgagg ccgccaggcc gcctcccgcg ccgcggagac
     1501 cgatgccagg gcccgggcgg tctcctccgg cccggccgga acgccggccg gacccgccga
     1561 gcggcaggcg ccgaggacga ggagcgcggc cgcgacggcc gtccgcctca cggctgcttc
     1621 gccgcggcgg ccgtcctctc gagcttggct cggatcgcag ctccgccgtc ttccggcccg
     1681 agggcgagcg cccgcgacca cctctccctc gccttcgcga gatcgcccct cttctcctcg
     1741 aggtccccga ggtggtcctc gatcgtcggg tcgtccggcg aaaggcgttt ggccgtcagg
     1801 aggtgcttct ccgcttcgtc gaggttgccg agccggaact ggatccatcc gagcgagtcg
     1861 aggtaggccg cgttcgacgg ctcgagggcg accgccctcg tgacgagctc gagggcccga
     1921 tccaggttct cgttacggtc cgcgaacatg tagccgaggt agttgagggc cgcgccgtgg
     1981 tccggcttca gcgagatcag cttctcgaac gccgcgatgg actccgggac cttcttgtcc
     2041 cgctcgagcg acgccgcctg ccggaagagg aggtccgggt cgtcgggaaa ggccgcgagt
     2101 cccgcagagg ccgcatccgc ggcgcgcccg taccgctcga gccgctgcca gacgtcggcg
     2161 gcagcgagcg ccccttcgcg cccctccctc gagagggccg cgaccatctc ctccccttcc
     2221 gcgcgctcct tctccttctc cgagcggagg aggaactcgg ccaggagccc gcggacgtcg
     2281 aggtccttcg gcgccttcgc gaacgcctcg cggcaggcct gaagcccctg cgccggctcc
     2341 ttcgcgtctc gcgcgacgag cgtcaggagg ttcagggcct gcatgttcac cgacccgtcc
     2401 tcctcgagga ggtgcgggcg aacggcctcc cgcgccgccg cccagtcctt ccggaggtag
     2461 gcgacgtagc cgagctggcc gtcgacggag acgatcccgc cggccggccc cttccgcgcg
     2521 acgagctggg agcggaggtc ccggaggacc gtctccgcct cgtcgagcct ccgcgcctcg
     2581 aggaagttga gggcgcgccg gaacgcgacg tcggcgtcgt cgggctcgag gccctggagc
     2641 ttcttcagga gctcctccgc acgcgcggtc tccagcttct cggacaggag cgccgcgtag
     2701 tgccggaggg cgtcgcgatg atccgggtcg accttcagca cctcctgaaa cgcctcctcg
     2761 gcctcggaga gccggcgggc acgaaggagc gagaggccgt actgcgcgcg aaggccgagg
     2821 ttccccggct gccgcttgac gagctccgcc aggatcggca gcgccttgtc gaacttccgg
     2881 gtcgcctggt agacgcgcag gaggccgaag gaggccgcgg cgttctgcgc gtccatgcgg
     2941 aggatcccga gatagggctc ctcggcctcc tcggggcgcc cgctcttctc gagcgcctcg
     3001 ccgtagagga gaggcaggag aggcccccgg acgttctgct ggactcggga gaggacggcg
     3061 gccgcgtcct cgtacctttc gagccgcagg taggcctggc cgagcgcgag ggcgcccgcc
     3121 ggctccatcg gctgaaggtc gttcgccgtc ttcagcggcg cgatcgcccc ctcgatcccg
     3181 ttccggtcac gggccgccgc caggaggacc tgcccgagga cccggtgcgc ggcggcgctc
     3241 ccggggacga gctcgacggc ctttcgggcc tgctgctcgg cctccggcag gatgccgaag
     3301 tcgcgcagcg cctccgcgta ctcgcggcga agatggccgt ccgcagggtc cagctccacg
     3361 gcccgccgga attccttgag ggcctcctgg aactccccct ccgctgcgaa gagccgggcc
     3421 cggaggaatc gctcgacggc gtcgcgccgg gccgactcgt ccgggctgag cgcctcggag
     3481 cgggccggcg gcgccgccgc ggcgggcggg gcgaggatcg caagggcgag ggcggcggcg
     3541 gcggggcgaa atctcatgcc gccgcggagc gtagcaagcg ggaggccagg aagggaaagg
     3601 ccccccgctc acgacttgac gacctcagaa gatcgcaggc tcctgccagg tctcgaaaac
     3661 ctcgcgcatg acgtcggaaa tctcaccgac cgtcgcgtag gctttgaccg cctcgacgag
     3721 agggggcatg acgttcgcgc cgttccggca cgcctcgcgg agggcgtgga gcgtcgagac
     3781 gaccgccttc ccgtcccgcg tccggcgaac ctcccggagc tgcgcgacct gctccgccgc
     3841 cgtctgctcg gagatgacga gcgtcgggac ctcgtcctcc gggacctcct ccgtgaaggc
     3901 attcacgccg accatgacct tgtcgccgac gtcgaaggcc cgctggaagc ggtaggcggc
     3961 gtccatgatc tccttctgcg ggaagccggc ctcgatcgcc tcgaccattc cgccgtaggc
     4021 gtcgatcttc cggaagtagt cgaacgcccc ctcctccatc cggttcgtcg cctcctccac
     4081 gaagtacgcc ccgccgagcg ggtcgatcgt gtgcgcgacg ccggtctcgt gcatgaggat
     4141 ctgctgggtc cgcaccgcga ccttcgcggc gaactcggtc gggagcgcga gcgtctcgtc
     4201 gagggcgttc gtgtggagcg attgggttcc gccgaggacg gcggcgaggg cctggatcgc
     4261 gacgcgggcg acgttgttgt agggctgctg ggccgtgagc gagacgcccg ccgtctgcgt
     4321 gtggaagcgg agcttccacg actcggggct cttcgcgccg taccggtcgc gcatcacctt
     4381 cgcccagatc cgccgcgccg cgcggaactt cgcgatctct tcgaaaaagt cgttgtgcgc
     4441 gttgaagaag aacgacaggc gcggcgcgaa ctcgtcgacg gagaggccgg cgtcgacgcc
     4501 gtacttcacg tactccatcc cgtcgcggag ggtgaaggcg agctcctgaa gagccgtcga
     4561 gcctgcttcc cggatgtggt agccggaaat ggacacggtg ttccacttcg ggacgtgctt
     4621 cgcgcagaag gagaactggt ccgtcacgag cctcatcgaa gggcgcggcg gaaagatgaa
     4681 ctccttctgc gcgatgtatt ccttgaggat gtcgttctgg agcgtcccgc cgaccttcga
     4741 ccagggagtc ccctgcttct ccgccaccgc gatgtacatc gcgagggcga tcggcgccgt
     4801 ggagttgatc gtcatcgacg tcgtcaccgt accgaggtcg atcccctcg
//
LOCUS       DAOTEZ010000340         4826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_12758_length_4826_cov_8.046741, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000340 DAOTEZ010000000
VERSION     DAOTEZ010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12758_length_4826_cov_8.046741"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="P5164_15275"
     CDS             <1..195
                     /locus_tag="P5164_15275"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HRY45298.1"
                     /translation="AEAEKGIREALEARTGTRVRLRTELDPSLLSGFVVRLGSEVFDA
                     SLARRLARARKALEGAAGAA"
     gene            227..1795
                     /gene="atpA"
                     /locus_tag="P5164_15280"
     CDS             227..1795
                     /gene="atpA"
                     /locus_tag="P5164_15280"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRY45299.1"
                     /translation="MTDISAREITQLIKAQLAGLDRGIDTAEVGTVLSVGDGIARVYG
                     LDKVMAGELLEFPNDVFGLALNLEEEDVGCVLMGDTAKIREGHEVRRTKRIISVPVGP
                     AVIGRVVDPLGQPLDGKGPIEAKEYYPIERIAPGVVARQPVKEPMQTGLKAIDAMIPI
                     GRGQRELIIGDRQTGKTSVALDAIINQKGKGVVCIYVAIGQKNSTVAQVVQTLETHGA
                     MEHTIVVAASASDPAPLQYIAPYAGCAMGEYFQFTGKDGKPASKDNPGGHVLVIYDDL
                     SKQAASYREISLLLRRPPGREAYPGDVFYLHSRLLERASKLNDDLGGGSLTALPIIET
                     QANDVSAYIPTNVISITDGQIFLESDLFYSGQRPAINVGISVSRVGGSAQVRTMRTVS
                     GTLRLDLAQYRELAAFAQFGSDLDKATQSQLNRGKRLTEILKQNVFVPMDVALQVASV
                     FAGTKGFLDDLRVEAVLPFEKTLHRTLVSEKAALLDEVRTAKKMTKELEEALAAAVTE
                     AKKLFLADNPSAKA"
     gene            1809..2690
                     /gene="atpG"
                     /locus_tag="P5164_15285"
     CDS             1809..2690
                     /gene="atpG"
                     /locus_tag="P5164_15285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HRY45300.1"
                     /translation="MPNLIDIRRRIRSVKSTQQITKAMKMVAASKLRRSQERVLAARP
                     YARTLGATLASVAGRLPARGDGAPAHPLLAQREEKKVILVVVTGDKGLCGAFNTNVNR
                     VAGGFLREKRAAGVEVSLVTLGRKAVDFWKRRTVPVLDARPGLFQRFGYETAAEVARS
                     LAARFTGGEVDAVYVVHNEFRSVISQVVGTKRLLPIELGELGAAEGAGTDYLFEPGPD
                     VILGRLLPRHVEFQLFRVLLESNAAENAARMTAMESATKNAGEMIDSLTLTYNRARQA
                     KITKELIEIVSGAEALK"
     gene            2753..4198
                     /gene="atpD"
                     /locus_tag="P5164_15290"
     CDS             2753..4198
                     /gene="atpD"
                     /locus_tag="P5164_15290"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRY45301.1"
                     /translation="MAIEGKIVQVIGPVVDVEFPGGHLPAILNAVHVTDPDGLSTVGI
                     DLMTEVAQHLGENRVRCIAMKPADGLVRGMKAVDLGAPISVPVGPETLGRILNVIGEP
                     VDGLGEVATKERWPIHRHAPPYDEQTTAVEMFETGIKVIDLLEPYTKGGKTGLFGGAG
                     VGKTVLIMELINNVAKAAGGYSVFAGVGERTREGNDLWLEMTESGVIDPHDWKKSKAS
                     LIYGQMTEPPGARLRVGLTGLTVAEWFRDTEGKDVLLFIDNIFRFTQAGSEVSALLGR
                     MPSAVGYQPNLATEMGELQERITSTKKGSITSVQAIYVPADDLTDPAPATAFAHLDAT
                     TVLSRQIAELGIYPAVDPLASTSKILSPMVVGEEHYAVARQVQQILQKYKDLQDIIAI
                     LGIDELSEDDKLVVARARKIQRFLSQPFHVAEQFTGIPGKYVKIADTIRSFKEIVEGK
                     HDEVPEQAFLLQGTIDDVLERYERMKKAGEA"
     gene            4203..4616
                     /gene="atpC"
                     /locus_tag="P5164_15295"
     CDS             4203..4616
                     /gene="atpC"
                     /locus_tag="P5164_15295"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HRY45302.1"
                     /translation="MSAPGRLTLTVVTPERSVVERAACDAVSLPGELGELGILPGHTP
                     LVSLLAVGRVVWTDAGRKTSVAVRGGFAEVADDSVRVLADLAAEKDAIDAAEAREELG
                     AGDARRLAVVGQEQLDAVNADVAFAEAKLAVAQGG"
     gene            4621..>4826
                     /locus_tag="P5164_15300"
     CDS             4621..>4826
                     /locus_tag="P5164_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45303.1"
                     /translation="MADETNERVHEAFDAVRRDLDDRLGVEERAAVERLRDAAAEGDA
                     VRAREELAAVKERHGWLHEELVKHP"
BASE COUNT          791 a         1769 c         1632 g          634 t
ORIGIN      
        1 gccgaggccg agaaggggat ccgcgaggcc ctcgaggcgc gcacgggcac gcgcgtgagg
       61 ctccgcaccg aactcgaccc ctcgctcctg tcgggcttcg tcgtccggct cgggagcgag
      121 gtcttcgacg cctccctcgc gcgccgcctc gcgcgggcgc ggaaggccct cgagggcgcc
      181 gcgggcgccg cgtgaccacc acaggacgag gaaaagagac gccgcaatga ccgacatcag
      241 cgcacgcgaa atcacgcagc tcatcaaggc gcagctcgcc gggctcgacc ggggcatcga
      301 cacggccgag gtcgggacgg tcctctccgt cggcgacggc atcgcccgcg tctacggcct
      361 cgacaaggtc atggcgggcg agctgctcga gtttccgaac gacgttttcg gcctcgccct
      421 gaacctcgag gaggaggacg tcggctgcgt cctgatgggc gacaccgcga agatccgcga
      481 ggggcacgag gtccgccgga cgaagcggat catctccgtc ccggtcgggc cggccgtcat
      541 cggccgcgtc gtcgacccgc tcggacagcc gctcgacggc aaggggccga tcgaggcgaa
      601 ggagtactac ccgatcgagc ggatcgctcc cggcgtcgtc gcgcgccagc cggtcaagga
      661 gccgatgcag accgggctga aggcgatcga cgcgatgatc ccgatcggcc gcggccagcg
      721 cgagctgatc atcggcgacc gccagacggg gaagacctcg gtcgccctcg acgccatcat
      781 caaccagaag gggaagggcg tcgtctgcat ctacgtcgcg atcgggcaga agaactcgac
      841 ggtcgcgcag gtcgtccaga cgctcgagac ccacggcgcg atggagcaca ccatcgtcgt
      901 cgccgcgagc gcgtcggacc cggcccccct ccagtacatt gccccctacg ccggctgcgc
      961 gatgggggag tacttccagt tcacgggcaa ggacgggaag cccgcgagca aggacaaccc
     1021 cggcggccac gtcctggtga tctacgacga cctctcgaag caggcggcct cctaccgcga
     1081 gatctcgctc ctcctccgcc ggccgccggg ccgcgaggcg tacccgggcg acgtcttcta
     1141 cctccacagc cggctcctcg agcgcgcctc gaagctgaac gacgacctcg gcggcggatc
     1201 gctcacggcg ctcccgatca tcgagacgca ggcgaacgac gtctccgcct acatcccgac
     1261 gaacgtcatc tcgatcacgg acggacagat cttcctcgag tcggacctct tctactcggg
     1321 ccagcgcccg gcgatcaacg tcggcatctc ggtctcccgc gtcggcgggt cggcgcaggt
     1381 ccggacgatg cggaccgtct ccggcacgct ccgcctcgac ctcgcccagt accgcgagct
     1441 cgccgccttc gcccagttcg gctccgacct cgacaaggcg acccagagcc agctgaaccg
     1501 cggcaagcgc ctcacggaga tcctcaagca gaacgtcttc gtcccgatgg acgtcgccct
     1561 tcaggtcgcc tcggtcttcg ccgggacgaa gggcttcctc gacgacctgc gcgtcgaggc
     1621 ggtcctgccg ttcgagaaga cgctccaccg gaccctcgtc tccgagaagg ccgccctcct
     1681 cgacgaggtc cggacggcga agaagatgac gaaggagctc gaggaggcgc tcgcggccgc
     1741 cgtgacggag gccaagaagc tcttcctcgc cgacaacccg agcgcgaagg cctgacgccg
     1801 gagacgcgat gccgaacctg atcgacatcc ggcggcggat ccgcagcgtc aagtcgacgc
     1861 agcagatcac gaaggcgatg aagatggtcg ccgcgtcgaa gctccgccgg tcgcaggagc
     1921 gggtcctggc ggcacgcccc tacgcgcgca cgctcggcgc gacgctggcc tcggtcgcgg
     1981 gccggctccc ggcgcggggc gacggcgcgc ccgcccatcc gctcctcgcg cagcgcgagg
     2041 agaagaaggt gatcctcgtc gtcgtgacgg gcgacaaggg cctctgcggg gccttcaaca
     2101 cgaacgtgaa ccgcgtcgcc ggcggcttcc tccgggagaa gcgggccgcg ggcgtcgagg
     2161 tttcgctcgt gacgctcggc cgcaaggccg tcgacttctg gaagcgccgg acggtccccg
     2221 tcctcgacgc gcgtcccggc ctcttccagc ggttcggcta cgagacggcc gccgaggtgg
     2281 cccggtccct cgccgcccgc ttcaccggag gcgaggtcga cgccgtctac gtcgtccaca
     2341 acgagttccg cagcgtcatc tcccaggtcg tcggaacgaa gcggctcctc ccgatcgagc
     2401 tcggggagct cggcgcggcc gaaggcgcgg ggacggacta cctcttcgag cccggcccgg
     2461 acgtcatcct cgggcgcctc ctcccgcggc acgtcgagtt ccagctcttc cgcgtcctcc
     2521 tcgagtcgaa cgccgcggag aacgccgcgc gcatgacggc gatggaatcg gcgacgaaga
     2581 acgcgggcga gatgatcgac tcgctgaccc tcacctacaa ccgcgcgcgc caggcgaaga
     2641 tcacgaagga gctcatcgag atcgtctccg gcgccgaggc cctgaagtga ggccctcccc
     2701 cgtctccccg tcccacgagt actcccggcc ccgagggccg cagaggtagc acatggccat
     2761 cgaaggcaag atcgtccagg tcatcggccc cgtcgtcgac gtggagttcc cgggcgggca
     2821 cctgcccgcc atcctgaacg ccgtccacgt caccgacccg gacggcctct ccacggtcgg
     2881 gatcgacctg atgaccgagg tcgcccagca cctcggcgag aaccgggtcc gctgcatcgc
     2941 gatgaagccg gccgacggcc tcgtgcgggg catgaaggcg gtcgacctcg gcgcgccgat
     3001 ctcggtcccc gtcggacccg agacgctcgg gcgcatcctg aacgtcatcg gcgagccggt
     3061 cgacggcctc ggagaggtcg ccacgaagga gcgctggccg atccaccggc acgccccgcc
     3121 gtacgacgag cagacgaccg cggtcgagat gttcgagacg gggatcaaag tcatcgacct
     3181 cctcgagccg tacacgaagg ggggcaagac gggcctcttc ggcggcgcgg gcgtcggcaa
     3241 gaccgtcctc atcatggagc tcatcaacaa cgtcgcgaag gccgcgggcg gctactcggt
     3301 cttcgcgggc gtcggcgaac ggacccgcga ggggaacgac ctctggctcg agatgaccga
     3361 gtcgggcgtc atcgacccgc acgactggaa gaagtcgaag gcctcgctca tctacggcca
     3421 gatgacggag cccccgggcg cgcgcctgcg cgtcggcctg acgggcctta cggtcgccga
     3481 gtggttccgc gacacggaag ggaaggacgt cctcctcttc atcgacaaca tcttccgctt
     3541 cacgcaggcg ggctccgagg tctccgcgct cctcggccgc atgccctccg ccgtcggcta
     3601 ccagccgaac ctcgcgaccg agatgggcga gctgcaggag cggatcacct cgacgaagaa
     3661 ggggtcgatc acgtcggtcc aggcgatcta cgtcccggcc gacgacctga ccgacccggc
     3721 tccggcgacc gcgttcgccc acctcgacgc gacgaccgtc ctctcgcggc agattgccga
     3781 gctcggcatc tacccggccg tcgacccgct cgcctcgacc tcgaagatcc tctccccgat
     3841 ggtcgtcggc gaggagcact acgccgtcgc gcggcaggtc cagcagatcc tccagaagta
     3901 caaggacctc caggacatca tcgcgatcct cggcatcgac gagctctccg aggacgacaa
     3961 gctcgtcgtc gcccgcgccc ggaagatcca gcgcttcctc tcgcagccgt tccacgtcgc
     4021 cgagcagttc accggcatcc cgggcaagta cgtcaagatc gccgacacca tccgctcgtt
     4081 caaggagatc gtcgagggca agcacgacga ggtgcccgag caggcgttcc tcctgcaggg
     4141 gacgatcgac gacgtcctcg agcgctacga gcggatgaag aaggcgggcg aggcctgacc
     4201 cgatgtccgc gccgggccgc cttacgctga cggtcgtcac gccggagcgc tccgtcgtcg
     4261 agcgcgccgc gtgcgacgcc gtgtcgctcc cgggcgagct cggcgagctc ggcatcctcc
     4321 cgggccacac gccgctcgtc tccctcctcg ccgtcggccg ggtcgtctgg accgatgcgg
     4381 ggcggaagac ctccgtcgcg gtccgcggcg gcttcgccga ggtcgcggac gactcggtcc
     4441 gggtccttgc ggacctcgcg gccgagaagg acgcgatcga cgccgccgag gcgcgcgagg
     4501 agctcggagc gggcgatgcg cggcgcctcg ccgtcgtcgg gcaggagcag ctcgacgcgg
     4561 tgaacgccga cgtcgccttc gccgaggcga agctcgccgt cgcgcagggg ggctgacgcc
     4621 gtggccgacg agacgaacga aagagtccac gaggcgttcg acgccgtccg gcgcgacctc
     4681 gacgaccgcc tcggcgtcga ggagcgcgcc gccgtcgagc gcctccgaga cgccgcggcc
     4741 gagggcgacg ccgtccgcgc ccgcgaggag ctcgccgcgg tgaaggagcg gcacggctgg
     4801 ctccacgagg agctcgtgaa gcaccc
//
LOCUS       DAOTEZ010000341         4814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_12815_length_4814_cov_6.319395, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000341 DAOTEZ010000000
VERSION     DAOTEZ010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12815_length_4814_cov_6.319395"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="P5164_15305"
     CDS             complement(<1..802)
                     /locus_tag="P5164_15305"
                     /inference="COORDINATES: protein motif:HMM:NF015148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HRY45304.1"
                     /translation="MAEPKPDAAPAAFEDVCLPDGAFRPLAPGETYEPVVPAGASVPE
                     VTVRSVAQGFLWSVVFSAAATYIALKLGQGIESAIPISILAVGASVLFVKLLAARAST
                     LLENVNVLAVGATSGIIAGGSVFTMPAIHILGLEGRSSFWQIFLVPLLGAVLGVFFLA
                     PFRRYFVRDLHGKLPYPEARATTEILVAGKRGGRSAVVLSWSAGLAAAFDFVGPSMKG
                     WAENFSTAAIPALSGFTSRFKAVFTMNTSAAVFGLGYIMGLDYAAIIMA"
     gene            867..1496
                     /locus_tag="P5164_15310"
     CDS             867..1496
                     /locus_tag="P5164_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018511592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HRY45305.1"
                     /translation="MPFDPAPPRREPPVLLALAFAALVVSGIGPKDRFTWLLEVAPVL
                     IGAPILVATVRRFPLTPLLYRLLFLHALVLMLGGHYTYAEVPLGEWAKEAFGLARNHY
                     DRLGHLMQGFVPAVLAREVFLRRSPLRPGGWLRLAVTSVCLAFSALYELIEWAAALAT
                     GEAATAFLGTQGDVWDTQWDMFLALCGALLAQALLARLHDRQLARLPRA"
     gene            complement(1566..1895)
                     /locus_tag="P5164_15315"
     CDS             complement(1566..1895)
                     /locus_tag="P5164_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45306.1"
                     /translation="MKKTTAVLLVAVLAIVLGSAAVATMDMQKDMNAKYPTAKAKCAT
                     CHVKAMAKKGDAEVNAFGKDMMKFVVDPKAEKKTFDWAKLEPLDSDGDGQKNVDELKA
                     GTNPGAK"
     gene            complement(2139..2330)
                     /locus_tag="P5164_15320"
                     /pseudo
     CDS             complement(2139..2330)
                     /locus_tag="P5164_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954901.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
     gene            2645..3058
                     /locus_tag="P5164_15325"
     CDS             2645..3058
                     /locus_tag="P5164_15325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45307.1"
                     /translation="MRVPTRFLLVALVALVLAPALVAQEGGRIDTAIASDSRETMAER
                     DSFPPTQPKIYVFAMVADAAPDTPVRAVWIAEKCDGQPPNTKFAERTTKFPGGATWIP
                     FSYTKPNPQWASGAYRVELYLGKQLAATVKFSVTK"
     gene            complement(3048..3671)
                     /locus_tag="P5164_15330"
     CDS             complement(3048..3671)
                     /locus_tag="P5164_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017247902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HRY45308.1"
                     /translation="MRHLLRYFLQGLLVFVPSVLTIAIVVFVFRAVDAWMGLPVPGAG
                     FLATIALIVVVGALASNFVTKRLFELVEGVFRRLPFVKLLYSAIRDVTDAFVGERKGF
                     ERPVFVTLDEASGLKAVGFLTRDSLDGAGLADHVGVYLPQSYNFAGQLLVVPRRLVTP
                     VSARGADAMAFIVSGGAAELRGGAQSEAAAPEGRPRADLEAPGGATS"
     gene            3800..>4814
                     /locus_tag="P5164_15335"
     CDS             3800..>4814
                     /locus_tag="P5164_15335"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRY45309.1"
                     /translation="MPETFRSVPQMLLRRVAKTPDREAYLRPVPDGWEALSWREVGDR
                     VRAIACGLRALGLEDEERASILASTRLEWILADLGILSAGGATTTIYPSTTTPDALFI
                     LQDSDTRVLFAENPEQAERIARERSKVPGLKAIVLLEGDAAADGFVLTTETLMGKGRA
                     WDAANPGRFEALVEAVRPDALATLMYTSGTTGRPKGVELTHDCWVYEAEGIDALGLLT
                     EDDRQFLWLPLAHSFGKVLEAAQLRIGFSTAVDGRVEKIVENLGQVRPTFVAAVPRIF
                     EKIYNKIVGGAKEAGGAKWAIFSWAVGVGREVSKLRLAGKEPAGLLALKHKLAHKLVF
                     SKLH"
BASE COUNT          721 a         1718 c         1691 g          684 t
ORIGIN      
        1 ccgccatgat gatcgccgcg tagtcgagcc ccatgatgta gccgaggccg aagacggcgg
       61 ccgaggtgtt catcgtgaag accgccttga agcggctcgt gaagccggag agggccggga
      121 tcgcggcggt cgagaagttc tcggcccacc ccttcatcga cggcccgacg aagtcgaacg
      181 ccgccgccag ccccgccgac caggagagga cgacggcgct ccggccgccc cgcttccccg
      241 ccacgaggat ctccgtcgtc gcccgcgcct ccgggtacgg gagcttcccg tggaggtccc
      301 tcacgaagta gcggcggaac ggcgcgagga agaagacgcc gaggaccgct ccgaggaggg
      361 gcaccaggaa gatctgccag aacgacgacc ggccctcgag gccgaggatg tggatcgccg
      421 gcatcgtgaa gacggagccc cccgcgatga tcccggaggt cgccccgacg gcgaggacgt
      481 tcacgttctc gaggagcgtc gaagcgcgcg cggcgaggag cttcacgaag aggaccgagg
      541 cgccgacggc caggatcgag atcgggatcg cggactcgat cccctgcccg agcttcagcg
      601 cgatgtaggt cgccgcggcc gagaagacga ccgaccagag gaacccctgc gcgacggagc
      661 ggacggtcac ctccgggacg gacgcccccg cggggacgac gggctcgtac gtctcgcccg
      721 gggcgagcgg gcgaaacgcg ccgtcgggga ggcagacgtc ctcgaacgcg gcgggggcgg
      781 catcaggctt cggctcggcc atccgggaat gatgccgcag ggacggtcgc ccggcgccgt
      841 ctggtctaat cggcgcggag gtccccgtgc cgttcgaccc cgcaccgccg cggcgcgagc
      901 cgcccgtcct cctcgcgctc gcgttcgccg cgctcgtcgt ctccggcatc ggcccgaagg
      961 accggttcac ctggctcctc gaggtcgcgc cggtgctgat cggcgccccg atcctcgtcg
     1021 cgacggtccg gcgcttcccg ctcacgccgc tcctctaccg gctcctcttc ctccacgccc
     1081 tcgtcctgat gctcggcggg cactacacct acgcggaggt accgctcggg gagtgggcga
     1141 aggaggcgtt cggcctcgcg cgaaaccact acgaccggct cgggcacctg atgcagggct
     1201 tcgtcccggc ggtcctggcg cgcgaggtct tcctccgccg ctcgccgctc cggcccggcg
     1261 gctggctccg cctcgccgtc acctcggtct gcctcgcgtt cagcgcgctc tacgagctga
     1321 tcgaatgggc ggcggcgctc gcgacgggag aggcggcgac cgccttcctc ggaacgcagg
     1381 gcgacgtgtg ggacacgcag tgggacatgt tcctcgccct ctgcggcgcc ctcctcgcgc
     1441 aggcgctcct cgcgcgtctc cacgaccgcc agctcgcgcg gctcccgcgc gcctgaatcc
     1501 cggaagacga aaaggccggg cgcccgaaag cgtccggccc gttctcgcgc gaggcgcgag
     1561 gggtcttact tggcgccggg gttcgtgccg gccttgagct cgtcgacgtt cttctggccg
     1621 tcgccgtcgc tgtcgagcgg ctcgagcttc gcccagtcga acgtcttctt ctcggccttc
     1681 gggtcgacga cgaacttcat catgtccttg ccgaacgcgt tcacctcggc gtcgcccttc
     1741 ttggccatcg ccttcacgtg gcaggtggcg cacttcgcct tcgccgtggg gtacttcgcg
     1801 ttcatgtcct tctgcatgtc catcgtggcg acggcggccg agccgagcac gatcgcgagg
     1861 acggcgacga ggaggacggc ggtggtcttc ttcatctcgt ggctccttcc ggtggggctg
     1921 agccccttct cgagtccttc gttttcaaga tccctgccat ccgggaaagg ggacgtttcg
     1981 gcgctcccgg ccccccgatc tcccaaacgc gacgcatcga gacggcgtcg cggaatcagc
     2041 cgagcgggat gccacgcccg acccggacga ggccgacgaa gtagaggtcg tcgccggcct
     2101 gccggtcgcc cctacgccac cgccgggacg atcgagccct cggtcgggca gacctcgacg
     2161 cacttcggcg agtcgtagtg cccctggcac tccgtgcact tcgcggcgtc gatgacgtag
     2221 gtcatgtcgc ccgggctgat cgcctggttc gggcactcgg gctcgcaggt cccgcagctg
     2281 atgcagttct cggtgatcat catcgccatg acggcctcct cgtttccggg ggacgccttc
     2341 tccctgcccc ccgcggagcc ggaccacggg cgcggaccgg caaccgcgag aggctagggg
     2401 aggagcgtgg ccggcacaat gatgcgaacg gatcattccg aaacggctcc ggcggagccg
     2461 cgtatcccgt tgacatcacg ccggatcggg ttccaaattc cctgtcgtgg ccgcgcgtcc
     2521 gaaggaccgg caggtggtcg tggcggcaga ggcgtcggcg gggcaggcgg cggagcgagc
     2581 cgccgaaagc gagacggaga gtgccggcgt ccgacccgat ccggaagcac gaaaggagac
     2641 cgaagtgaga gttccgaccc gattcctcct cgtcgcactc gtcgccctcg tcctggcgcc
     2701 ggccctcgtc gcccaggaag gggggcgcat cgacaccgcc atcgcgtccg acagccgcga
     2761 gacgatggcc gagcgcgact cgttccctcc gacgcagccg aagatctacg tcttcgcgat
     2821 ggtcgccgac gcggcgccgg acacgccggt ccgggcggtc tggatcgcgg agaagtgcga
     2881 cggccagccc ccgaacacga agttcgccga gcggacgacg aagttccccg gcggcgcgac
     2941 ttggatcccc ttctcctata cgaagccgaa cccgcagtgg gcctccggcg cctaccgcgt
     3001 cgagctctac ctcggcaagc agctcgcggc gacggtgaag ttctcggtca cgaagtagcg
     3061 cctccgggcg cctcgaggtc cgcgcgcggc cgcccttccg gggcggccgc ttcgctttgt
     3121 gcgccgccgc ggagctctgc ggccccgccc gagacgatga aggccatcgc gtcggccccg
     3181 cgcgccgaga ccggcgtcac gagccgccgc gggacgacga ggagctgccc ggcgaagttg
     3241 tacgactgcg gcaggtagac gccgacgtgg tcggcgaggc cggcgccgtc gagcgaatcc
     3301 cgcgtcagga agccgaccgc cttcaggccg ctcgcctcgt cgagcgtgac gaagaccggc
     3361 cgctcgaacc ccttcctctc gccgacgaac gcgtccgtca cgtcgcggat cgccgagtag
     3421 aggagcttca cgaacgggag gcgccggaag actccctcga cgagctcgaa gaggcgcttc
     3481 gtgacgaagt tcgaggcgag cgccccgacg acgacgatga gcgcgatcgt ggcgaggaac
     3541 cccgccccgg ggacggggag ccccatccat gcgtcgaccg cccggaagac gaagacgacg
     3601 atcgcgatcg tcaggaccga agggacgaag acgaggagcc cctggaggaa gtatcggagg
     3661 aggtggcgca tggcgcgggg agtctacggg tccgcgccgc ctcggcggcg taacgggctt
     3721 ccgggcgcgc tagcgcggtc ggcggccgct ctcgtggtag aaccgtctcc gcccggagaa
     3781 cgaaccgagg aggagtgccg tgcccgagac gttccgctcc gtcccgcaga tgctgcttcg
     3841 gcgcgtcgcg aagacgcccg accgcgaggc ctacctgcgc cccgtcccgg acggctggga
     3901 ggccctttcc tggcgcgagg tgggcgacag ggtccgcgcg atcgcgtgcg gcctgcgcgc
     3961 gctcggcctc gaggacgagg agcgcgcctc gatcctcgcg agcacccggc tcgagtggat
     4021 cctcgccgac ctcgggatcc tctcggcggg cggcgccacg acgacgatct atccctcgac
     4081 gacgacgccc gacgccctct tcatcctcca ggactcggac acccgggtcc tcttcgccga
     4141 gaacccggag caggcggagc ggatcgcgcg ggagcgctcg aaggtgcccg gcctgaaggc
     4201 gatcgtcctc ctcgaggggg atgcggccgc ggacggcttc gtcctgacga cggagaccct
     4261 catggggaag gggcgggcgt gggacgcggc gaaccccggc cggttcgagg cgctcgtcga
     4321 ggccgtccgc cccgacgccc tcgcgacgct catgtacacc tcggggacga cgggccggcc
     4381 gaagggcgtc gagctcactc acgactgctg ggtctacgag gcggaaggga tcgacgcgct
     4441 cggcctcctg acggaggacg accggcagtt cctctggctc ccgctcgccc attccttcgg
     4501 aaaggtcctc gaggcggcgc agctccggat cggcttctcg accgccgtcg acggacgggt
     4561 ggagaagatc gtcgagaacc tcggccaggt ccgcccgacg ttcgtcgcgg ccgtcccgcg
     4621 gatcttcgag aagatctaca acaagatcgt cggcggcgcc aaggaggccg ggggcgcgaa
     4681 gtgggccatc ttctcgtggg ccgtcggcgt gggccgcgag gtctcgaagc tccggctcgc
     4741 cgggaaagag ccggccgggc tcctcgccct gaagcacaag ctcgcgcaca agctcgtctt
     4801 cagcaagctc cacg
//
LOCUS       DAOTEZ010000342         4763 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_13011_length_4763_cov_5.999363, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000342 DAOTEZ010000000
VERSION     DAOTEZ010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13011_length_4763_cov_5.999363"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..1345)
                     /locus_tag="P5164_15340"
     CDS             complement(104..1345)
                     /locus_tag="P5164_15340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HRY45310.1"
                     /translation="MEPPFQVALLLAATAAACAAAFHAGRRAAASPLRRQLLEAERRD
                     ETRAEEDRQLSARLGRANAALTRRAAALERVLRATPELRAHLPLESVLRHTVEAARDS
                     LGHRHVLLSLHDRLEGALVPRAHVGLEEAWASLAEVRVPAGPLAHDLAAPETAEAAPL
                     RSLRRRLGLAPPVAVPLAAGSRVVGLLELAEPGTGAVDAEEDRVVLDLFALQAVQAVR
                     LARAYRTVRLGTLRDALTGVANHGSFQETLRHEIARHGRSAESLVLLMVDLDDFKAVN
                     DRFGHPVGDAVLRGVVARLLESVREMDVVARYGGEEFAVVLPQTDPAQGARVAERLRA
                     AVAAAPLPAGPEEPLPLTVSIGLAVYPEDAVTKGALVECADRALYAAKRTGKNRVVRF
                     TRVPSAADDLLAAPRRAPRDA"
     gene            complement(1359..2843)
                     /locus_tag="P5164_15345"
     CDS             complement(1359..2843)
                     /locus_tag="P5164_15345"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HRY45311.1"
                     /translation="MAITGNLRTMQLSELLQWLSMGQKTGTLAIRGGAGEKRIYFQNG
                     RIISSSSTIEREYLGHFLVSYGYITEEELTKAMEVQEESKILLGKILVMIGAIAEEDL
                     VELIRLKACETIYDIFLWPEGAFQFLDGEIPQRPMVPIAIDVTGIVMEGLRRFDEWQR
                     IRGRIRSLRDVPTIVAPVDVESLAEPHRLILRAIDGVRSLGEIAEATHNSDFPVARLV
                     FDLIESGHVELAPAPAVAAPKSSELTPEDPYETVFEEAATPHFEGVPDTADELPLPPP
                     PQEGRTAPSKDFARFLKRGPDSGSHRARPAGPVTTSFAAPVAAPPAPPPSTSFRPSAA
                     ASAGPPTSGSLRIGAPSAASLLEVGAPPSNVPWPAPVSGAFRVPAPAAGPSSASLRAP
                     APQGPPSSVSLRPPASPPSSQNKLAVPASASGSTFAPTAIPSLTRPMEELMSWSFTPN
                     EAFILSRINGLWDVRSIAKISPFPEGEVLRVFEKLHDGGLIRWR"
     gene            complement(2930..4117)
                     /locus_tag="P5164_15350"
     CDS             complement(2930..4117)
                     /locus_tag="P5164_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRY45312.1"
                     /translation="MAFQGVDFYQLDGLLSEEEIAVRDLVRDFVDNEVLPVIEDCAYE
                     GRFPAELVPKMAAMNLFGPTISEYGLPGLSNVAYGLITQELERGDSGLRSFASVQSSL
                     VMFPIWKYGSKEQKDRWIPALAKGEAIGCFGLTEPDFGSNPGGMRTVAKKDGESWVLN
                     GAKAWITNGTIADVAVVWAKVGGPDGRIHGFLVEKGTPGFTTSEHRMKMSLRASVTGQ
                     LAFEDCRIPAENVLPGVDGLKGPLSCLTQARYGISWGGIGSAMATYTCALDYAKSRVQ
                     FMGRPIAAHQLVQEKLAFMITEITKGQLLALQLGRMKDAGTMKPEHVSMAKRNNVWVA
                     RECAKLAREILGANGIVGEYPVFRHLANIESVFTYEGTHDIHTLVLGQAVTGIPSYNP
                     PAE"
     gene            4224..4475
                     /locus_tag="P5164_15355"
     CDS             4224..4475
                     /locus_tag="P5164_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45313.1"
                     /translation="MANPAWKTASDVTEGYLTLNGVMLRKYEPHELLSLQAELEKAAR
                     ELRGSIVPVDDVDGNQKKNRKLLRISQALTVLQAARSRR"
BASE COUNT          657 a         1647 c         1795 g          664 t
ORIGIN      
        1 ggggaggcgc gaccgctcct cgtcgggccg cgggtcggcg tcgactacgc cggcgaggcg
       61 gcggcctggc ccctcaggtt cgcggtcgag ggctgcccgg ccgtcacgcg tcgcgcgggg
      121 ctcgccgcgg agccgcgagc aggtcgtcgg ccgcggacgg gacgcgcgtg aagcggacca
      181 cgcggttctt ccccgtccgc ttggcggcgt agagggcccg gtccgcgcac tcgacgagcg
      241 ctcccttcgt cacggcgtcc tcgggataga cggcgagccc gatcgagacg gtgagcggga
      301 ggggctcctc gggcccggcc ggcagcgggg ccgcggccac cgccgcgcgg agccgctcgg
      361 ccacgcgggc cccctgcgcc gggtcggtct gcgggaggac gacggcgaac tcctcgccgc
      421 cgtaccgtgc gacgacgtcc atctcccgca ccgattcgag gagccgcgcg acgacgccgc
      481 gcaggaccgc gtcgccgacg ggatgcccga accggtcgtt gaccgccttg aagtcgtcga
      541 ggtcgaccat caggaggacg aggctctcgg cgctccgccc gtgcctcgcg atctcgtgcc
      601 ggagcgtctc ctggaaggat ccgtggtttg ccacgccggt cagcgcgtcg cgcagggtgc
      661 cgagacggac ggtccggtag gcgcgtgcga gccgtacggc ctggacggcc tggagtgcga
      721 agaggtcgag gacgacccga tcctcttccg cgtcgacggc gccggtcccc ggctccgcga
      781 gctccaggag cccgacgacg cgcgaccccg cggcgagcgg cacggcgacg ggcggggcga
      841 gcccgagccg ccgccggagc gaacggagcg gcgccgcctc ggccgtctcc ggggcggcga
      901 ggtcgtgggc gagcgggccg gcgggaacgc ggacttcggc gagcgacgcc caggcctcct
      961 cgaggccgac gtgggcccgc ggcacgagcg ccccctcgag gcggtcgtga agcgagagga
     1021 ggacgtgccg gtggccgagg ctgtcgcgcg cggcctcgac ggtgtggcgg aggaccgact
     1081 cgagcgggag atgcgcccgg agctccggcg tcgcgcggag gaccctctcg agcgcggcgg
     1141 cgcgacgggt cagcgcggcg ttcgcgcgcc cgagccgtgc cgacagctgc cggtcctcct
     1201 cggccctcgt ttcgtctcgc cgctccgcct cgaggagctg ccgccggagc ggggacgcgg
     1261 cggcccggcg accggcgtgg aacgcggctg cgcacgccgc cgccgtcgcg gcgagaagca
     1321 aggcgacctg gaagggcggc tccacggggc gcctccgctc agcgccagcg gatgaggccg
     1381 ccgtcgtgga gcttctcgaa gaccctgagg acctcgccct cggggaacgg gctgatcttc
     1441 gcgatcgagc ggacgtccca gagcccgttg atgcgggaga ggatgaacgc ctcgttcggc
     1501 gtgaacgacc aggacatcag ctcctccatc ggacgggtga gggaggggat cgccgtcggc
     1561 gcgaacgtgc tcccggaggc ggaggccggg accgcgagct tgttctgcga gctcggggga
     1621 gaggccggcg gccggagcga gacggaggag ggcggtccct ggggagccgg cgcgcgaagg
     1681 ctcgccgagg aggggcccgc cgctggagcc ggaacgcgga aagcacccga gaccggcgca
     1741 ggccacggga cgttcgaggg cggggcgccg acctcgagga gcgaggcggc cgagggggcg
     1801 ccgatccgca gggaacccga ggtcgggggg ccggcggagg ccgcggcgga gggccggaag
     1861 ctcgtcgacg gcggcggcgc ggggggcgcg gcgacgggag ccgcgaacga cgtcgtgacc
     1921 ggtccggcgg gccgtgcgcg atgggacccg ctgtcggggc cccgcttgag gaagcgggcg
     1981 aagtccttcg acggcgccgt ccggccctct tgcggcgggg gcggaagcgg cagctcgtcg
     2041 gcggtgtcgg ggacgccctc gaagtgcggc gtcgccgcct cctcgaagac cgtctcgtac
     2101 gggtcctcgg gggtcagctc cgagctcttc ggggcggcga cggcgggggc cggcgccagc
     2161 tcgacgtgcc cgctctcgat caggtcgaag acgaggcggg cgacggggaa gtcggagttg
     2221 tgggtcgcct cggcgatctc cccgagggag cggactccgt cgatcgcgcg gaggatcagc
     2281 cggtgcggct cggcaaggct ctcgacgtcg accggggcga cgatcgtcgg gacgtcgcgc
     2341 agcgaccgga tccggccccg gatccgctgc cactcgtcga accggcggag gccctccatg
     2401 acgattcccg tgacgtcgat ggcgatcggg accatcggcc gttgcgggat ctcgccgtcg
     2461 aggaactgga aggcgccttc gggccagagg aagatgtcgt agatcgtctc gcacgccttg
     2521 aggcggatca gctcgacgag gtcctcctcg gcgatcgcgc cgatcatcac gaggatcttc
     2581 ccgaggagga tcttcgactc ctcctggacc tccatcgcct tcgtcagctc ttcctcggtg
     2641 atgtagccgt acgagacgag gaagtgcccc aggtactcgc gctcgatcgt cgaggacgag
     2701 gagatgatcc ggccgttctg gaagtagatc cgcttctctc ccgcgccgcc ccggatggcc
     2761 agcgtgcccg tcttctgccc catcgagagc cactgcagca gctccgacaa ctgcatcgtc
     2821 cggaggtttc cggtaatggc catccgggag gcattctacg gggaggccgg ccctccgggg
     2881 cggctcgcgg accggcccgt ctccgggcgg gtccgcgggc cgcgtcgctt cactcggcgg
     2941 gcgggttgta ggaggggatg ccggtgacgg cctggcccag gacgagggtg tggatgtcgt
     3001 gcgtcccctc gtacgtgaag accgactcga tgttcgcgag gtgccggaag acggggtact
     3061 cgccgacgat cccgttcgcg ccgaggatct cgcgggcgag cttcgcgcac tcccgcgcga
     3121 cccagacgtt gttccgcttc gccatcgaga cgtgctcggg cttcatcgtc ccggcgtcct
     3181 tcatccgccc cagctggagc gcgaggagct gccccttcgt gatctccgtg atcatgaagg
     3241 cgagcttctc ctggacgagc tggtgggcgg cgatcggacg ccccatgaac tgcacgcgcg
     3301 acttcgcgta gtcgagcgcg caggtgtagg tcgccatggc ggagccgatg ccgccccagg
     3361 agatcccgta gcgggcctgg gtgaggcagg agagcggccc cttcagcccg tccacgcccg
     3421 ggaggacgtt ctccgccgga atccggcagt cctcgaaggc gagctggccc gtcacggagg
     3481 cccggagcga catcttcatc cggtgctcgg acgtcgtgaa tccgggcgtc cccttctcga
     3541 cgaggaagcc gtggatccgc ccgtcgggtc cgccgacctt cgcccagacg acggcgacgt
     3601 cggcgatcgt cccgttcgtg atccaggcct tggcgccgtt caggacccag gactccccgt
     3661 ccttcttcgc gaccgtgcgc atcccgcccg ggttcgagcc gaagtcgggc tcggtgaggc
     3721 cgaagcagcc gatcgcctct cccttcgcca gggccgggat ccagcggtcc ttctgctcct
     3781 tcgagccgta cttccagatc gggaacatca cgagcgagga ctggacggag gcgaacgacc
     3841 tgaggccgga gtcgccccgc tcgagctcct gcgtgatgag cccgtaggcg acgttcgaca
     3901 ggcccgggag gccgtactcg gagatcgtcg gcccgaagag gttcatcgcg gccatcttgg
     3961 ggacgagctc ggccgggaat cgcccttcgt aggcgcagtc ctcgatgacg gggaggacct
     4021 cgttgtcgac gaagtcccgg acgaggtccc ggacggcgat ctcctcttcg ctgaggagtc
     4081 cgtcgagctg gtagaaatcg acgccctgaa atgccacgaa ttcgcctcct gaggctctct
     4141 acggcccctc gccgtgcgcg gacgggagaa tctagcatgc ggccgttgac gggccggcgg
     4201 gcccggcgca tcctgtcgcc ccgatggcga atcccgcctg gaagacggcc tccgacgtga
     4261 ccgagggcta cctcaccctg aacggcgtaa tgctccggaa gtacgagccg cacgagctgc
     4321 tctcgctgca ggccgagctg gagaaggcgg cccgcgagct ccgggggtcc atcgtccccg
     4381 tcgacgacgt ggacgggaac cagaagaaga accggaagct cctccgcatc tcgcaggcgc
     4441 tgacggtcct gcaggcggcc cgttcgcgac ggtagttttc tcggggggac cacgcgcctc
     4501 tggcgcggcg gccggtcccc ccgaaccccc tccgagccgg cttcgcctga agggggacca
     4561 cgcgcctctg gcgcggcggc cggtccccct gaaccgcttc gggaagccgg ggcttgcggc
     4621 gcctgctaaa ttccgctgct atgtcgaccg gacctgtcgc ttccgtctcc gggctccgct
     4681 cccgcatcgg cgagaccgtc accctccggg gctggctcta caaccgccgg gacagcggga
     4741 agatccggtt cctcgtcttc cgc
//
LOCUS       DAOTEZ010000343         4756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_13034_length_4756_cov_7.534354, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000343 DAOTEZ010000000
VERSION     DAOTEZ010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13034_length_4756_cov_7.534354"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="P5164_15360"
     CDS             <1..773
                     /locus_tag="P5164_15360"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HRY45314.1"
                     /translation="VDVCDINMGCPANKILKGCAGAGLMRDLTRARGIIEACRKALPT
                     TPLTVKFRAGITEETTNFVELGKICEGEGVAAVALHPRTAKQFYTGRADWSRIARLKE
                     AVRIPVVGNGDVETPEDVLRMFRETGCDAVMIGRATMKNPWIFRQAADLLAGRPYRVA
                     TLEERRDVILQHFRLIREAEEDVPKRMMGKLKTFTGWYTHGVPKGTELRRRIQSLPTP
                     EAFLEAVESFFALRIDERDGRASAPEGAEAPPVRPVLA"
     gene            796..2673
                     /locus_tag="P5164_15365"
     CDS             796..2673
                     /locus_tag="P5164_15365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRY45315.1"
                     /translation="MRRVVSPLSVLLLLAASAGSAQPVQQTSGKVLGVSVPLEVSEQF
                     LGTSAGRTVVRFTLSFSRTDLREKAGLAPRVYPFFVAGEARSASGEVVDAFREPVDVD
                     LSDAELGKALRASFLRALPPGSITLGLRLEAENGRSVGLRSVTFDVPKMTSEFRADDS
                     GPLSAPAILLEEANREEAPSGGDLIRFLPPQREVPVGLLRIECEAKPPISRVEFHLGD
                     KLILARNRPPFTVEIDLGTIPRKQTLRALGFDKLGRFVDADAWALNEKEARLAVRVLD
                     LPKAKGKADSDVKVAVQSIAGAVAKRVALFLDDRKVAEWTSPPYRASVAAAEVAKATL
                     MRASAWDAEGKEYSDFRMLKGDERLLAKVDVNLVELNVSVFDAAGRFAKGLSKEDFTV
                     LEDGAVQDLAAFEFAESLPMALGLVLDGSGSMDKSMPLVKQAATEFVSNLVREKDQGF
                     VIEFRERPTLLAPLTSSRASLIRAIGETRAGGATAFHDSVILGLYQFRSLAGKKALVV
                     LTDGKDNHSSTDWPTLKRYARTAGVPIFVVGLDLGLFDMGLKSRLKELAEDTGGETFF
                     IGGAKELSDVYRKIETEVRSQYFLSYLTESKKKDDEFRTVEVRLARPGLRAKTIRGYF
                     P"
     gene            complement(2661..3560)
                     /locus_tag="P5164_15370"
     CDS             complement(2661..3560)
                     /locus_tag="P5164_15370"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRY45316.1"
                     /translation="MNPEALAARFAERLLLLDAALGTELARRGAPSAPPLWSARAVLE
                     RPDLVLAVHRENVAAGAHVLTAGTFRTQPALLEAAAREATRRAVALAREAASERPGVL
                     VAGSLAPVADCWRPDLVPPDAELARLHALHAGALAEAGADLLLLETFGTAREWLEAAR
                     AAAATGLAVVACATTREDGALLSGEPLGEAARALLALPAPPAALGVNCVPARRLAASL
                     DLLAAAAPGVPLAAYGNTGLPIDEATGAFAEPISPEEYAAVAERWLSLGARLVGGCCG
                     TGAGHVAALASRPGPPAGARYGK"
     gene            complement(3557..4309)
                     /locus_tag="P5164_15375"
     CDS             complement(3557..4309)
                     /locus_tag="P5164_15375"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRY45317.1"
                     /translation="MRFDALRRLLEKLQRSGKKTLDVEELSGDFTDSELAFGRLVLPL
                     VEDLDAMRKLLEDLSRRARAMQIVLDRLPVAALVLDAEGSLLAMNRAAKDHFGGAAIT
                     SAVLRAARRALQGTEEAAATVEDPRRRGGTLRLVSADVAEEKAEGPSEKPSVVFVVSN
                     DSPPPVDEADLVARFDLTKAEASVVALVAHGLANKEVGESMGLSVETVRSHLASAFRK
                     TGARNRAGLAALAYGARFGQGPISGAFPSLEE"
     gene            4368..>4756
                     /locus_tag="P5164_15380"
     CDS             4368..>4756
                     /locus_tag="P5164_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY45318.1"
                     /translation="MSHPKRNWALVLGASSGFGEAVSIALAKAGRNVFGVHLDRRATL
                     PNVERIAGEIEGAGGKAVFFNVNAADAEKRAEVLGAMKGALAENGGALDVVLHSLAFG
                     TLKPFLGDEKERMTKAQMEMTLDVMAHS"
BASE COUNT          673 a         1622 c         1824 g          637 t
ORIGIN      
        1 gggtcgacgt ctgcgacatc aacatgggct gcccggcgaa caagatcctg aaggggtgcg
       61 ccggagcggg cctcatgcgg gacctgacgc gggcgcgcgg gatcatcgag gcgtgccgga
      121 aggcgctccc gacgacgccg ctgacggtga agttccgggc cgggatcacg gaggagacga
      181 cgaacttcgt cgagctcggg aagatctgcg aaggggaagg ggtcgcggcg gtcgcgctcc
      241 acccgcggac ggcgaagcag ttctacacgg ggcgggccga ctggagccgg atcgcgcggc
      301 tgaaggaagc ggttcggatt cccgtcgtcg gcaacggcga cgtggagacg cccgaagacg
      361 tcctccggat gttccgcgag accggctgcg acgccgtgat gatcggccgg gccacgatga
      421 agaacccctg gatcttccgg caggcggcgg atctcctcgc cggccggcca taccgcgtcg
      481 cgacgctcga ggagcggcgg gacgtcatcc tccagcactt ccggctcatc cgcgaggcgg
      541 aggaggacgt cccgaagcgg atgatgggga agctgaagac gttcaccggc tggtacacgc
      601 acggcgtccc gaaggggacg gagctgcgcc gccggatcca gtcgctcccg actccggagg
      661 cgttcctcga ggcggtggag tcgttcttcg ccctccggat cgacgagcgg gacgggcggg
      721 cgtccgcccc ggagggcgcc gaagccccac ccgttcgtcc ggttcttgcg taagactcga
      781 cgggaggtcc tcgccgtgcg acgtgtcgtt tcccctctct ccgtcctcct cctgctggcg
      841 gcctccgccg gttccgccca gccggtccag cagacgtcgg ggaaggtcct cggcgtctcc
      901 gtcccgctcg aggtctccga gcagttcctc gggacgagcg ccggccggac ggtcgtccgc
      961 ttcacactct ccttctcgcg caccgacctg cgcgagaagg cggggctcgc cccgcgggtc
     1021 tacccgttct tcgtcgccgg ggaggcgcgg agcgcctcgg gcgaggtcgt cgacgcgttc
     1081 cgcgagccgg tcgacgtcga cctctcggac gccgagctcg gcaaggccct gcgcgcctcg
     1141 ttcctgcgcg cgctcccccc cggctcgatc acgctcgggc tgaggctcga ggcggagaac
     1201 ggccggtcgg tggggctgcg gtcggtgacg ttcgacgtgc cgaagatgac gtcggagttc
     1261 cgagcggacg actccggccc gctcagcgcc ccggcgatcc tcctcgagga ggccaaccgc
     1321 gaggaggcgc cgtcgggtgg cgacctgatc cgcttcctcc cgccgcagcg ggaggtgccg
     1381 gtgggtctcc tccggatcga gtgcgaggcg aagcccccga tctcccgggt cgagttccac
     1441 ctcggcgaca agctgatcct ggcccggaac cggccgccgt tcaccgtcga gatcgacctc
     1501 gggacgatcc cgcgcaagca gacgcttcgc gccctcggct tcgacaagct cggccgcttc
     1561 gtcgacgccg acgcctgggc gctgaacgag aaggaggcgc gcctcgcggt ccgcgtcctc
     1621 gacctgccga aggcgaaggg gaaggccgac tcggacgtga aggtggcggt ccagtcgatc
     1681 gccggcgcgg tcgcgaagag ggtcgccctc ttcctcgacg accggaaggt ggcggagtgg
     1741 acctcgcccc cctaccgggc gagcgtggcc gccgcggagg tcgcgaaggc gaccctgatg
     1801 cgggcgtcgg cgtgggacgc ggaggggaag gagtactccg acttccggat gctgaagggg
     1861 gacgagcggc tcctcgcgaa ggtggacgtg aacctcgtgg agctgaacgt ctcggtcttc
     1921 gacgcggcgg gccggttcgc gaaggggctc tcgaaggagg acttcacggt cctcgaggac
     1981 ggcgccgtcc aggacctcgc ggccttcgag ttcgccgagt cgctcccgat ggcgctcggc
     2041 ctcgtcctcg acggctcggg ctcgatggac aagtcgatgc ccctcgtcaa gcaggcggcg
     2101 acggagttcg tgtcgaacct cgttcgcgag aaggaccagg ggttcgtcat cgagttccgc
     2161 gagaggccca ctctcctcgc gcctttgacc tcgagccgcg cctcgctcat ccgggcgatc
     2221 ggcgagacgc gcgccggcgg cgcgacggcg ttccacgact cggtcatcct cggcctctac
     2281 cagttccgct ccctcgcggg aaagaaggcg ctcgtcgtcc tgacggacgg aaaggacaac
     2341 cactcctcga cggactggcc gacgctgaag cgctacgccc ggacggcggg ggtgccgatc
     2401 ttcgtcgtcg ggctcgacct cggcctcttc gacatggggc tgaagtcgcg cctgaaggag
     2461 ctcgcggagg acaccggcgg ggagaccttc ttcatcggcg gggcgaagga gctgtcggac
     2521 gtctaccgga agatcgagac ggaggtccgc tcgcagtact tcctgagcta cctgaccgag
     2581 tcgaagaaga aggacgacga gttccggacg gtcgaggtga ggctcgcccg gccgggcctc
     2641 cgcgcgaaga cgatccgcgg ctacttcccg tagcgcgcgc cggccggggg gcccgggcgc
     2701 gacgcgagcg ccgcgacgtg cccggcgccc gtcccgcagc agccgccgac gaggcgggcg
     2761 ccgagggaga gccaccgctc ggcgaccgcg gcgtactcct ccggcgagat cggctcggcg
     2821 aacgcgccgg tcgcctcgtc gatcgggagg cccgtgttcc cgtacgccgc gagcgggacg
     2881 ccgggcgcgg cggcggcgag gaggtcgagg ctcgcggcga gccgccgcgc cgggacgcag
     2941 ttgacgccga gggcagcggg gggcgcgggg agcgcgagga gcgcccgggc cgcctccccg
     3001 agcggctcgc ccgagaggag cgcgccgtcc tcgcgcgtcg tcgcgcaggc gacgacggcg
     3061 agaccggtcg ccgcggcggc tcgggcggcc tcgagccact cgcgcgccgt gccgaacgtc
     3121 tcgaggagaa gcaggtcggc gccggcttcc gcgagcgccc cggcgtggag cgcgtggagg
     3181 cgggcgagct ccgcgtcggg cgggacgagg tcgggccgcc agcagtcggc gacgggcgcc
     3241 agggagccgg cgacgaggac gccgggccgc tccgaggccg cctcgcgggc gagggcgacg
     3301 gcgcgccgcg tcgcctcgcg ggcggcggcc tcgaggaggg ccggctgcgt gcggaacgtt
     3361 cccgcggtca ggacgtgggc ccccgccgcg acgttctcgc ggtggacggc gaggacgagg
     3421 tcgggcctct cgaggaccgc gcgggcgctc cacagagggg gagccgaggg ggcgccgcga
     3481 cgtgccagct ccgtgccgag ggcggcgtcg aggagcagca gccgttcggc gaagcgcgcc
     3541 gcgagcgctt cggggttcac tcttcgaggc tggggaaggc gccggagatc ggcccctggc
     3601 cgaagcgggc gccgtaggcg agggcggcga ggccggcgcg gttgcgcgcg ccggtcttcc
     3661 ggaacgcgct cgccaggtga gaacggaccg tctcgaccga gagtcccatc gactccccga
     3721 cctccttgtt cgcgaggccg tgcgcgacga gggcgacgac ggaggcttcg gccttcgtca
     3781 ggtcgaaccg cgcgacgagg tcggcctcgt cgaccggggg aggcgagtcg ttcgagacga
     3841 cgaagacgac cgacggcttc tcggacggcc cctcggcctt ctcctcggcg acgtcggcgg
     3901 agacgaggcg gagcgtcccg cctcttcgac gggggtcctc gaccgtggca gccgcctcct
     3961 cggtcccctg gagggcgcgc cgcgccgctc ggaggacggc cgaggtgatc gcggcgccgc
     4021 cgaagtggtc cttcgccgcc cggttcatcg cgaggaggct cccctcggcg tcgaggacga
     4081 gcgcggcgac gggaaggcgg tcgaggacga tctgcatcgc gcgcgcgcgg cgcgagaggt
     4141 cctcgaggag cttcctcatc gcgtccaggt cctcgaccag ggggaggacg aggcggccga
     4201 acgcgagctc gctgtcggtg aagtcgcccg agagctcctc gacgtcgagc gtcttcttgc
     4261 cgctccgctg gagcttctcg aggagacgtc ggagggcgtc gaatcgcatc gaaccgggcc
     4321 tcgagcggca ctatacaggt ggggaggggg tgccgataat ccgcgccatg tcgcatccga
     4381 agcggaactg ggccctcgtc ctcggcgcgt cctcggggtt cggagaggcg gtctcgatcg
     4441 ccctcgcgaa ggcgggacgg aacgtcttcg gcgtccacct cgaccgtcgc gcgacccttc
     4501 cgaacgtgga gcggatcgcg ggcgagatcg aaggcgccgg agggaaggcc gtcttcttca
     4561 acgtgaacgc cgccgacgcg gagaagcgcg cggaggtcct cggcgcgatg aagggggctc
     4621 tcgcggagaa cggcggggcg ctcgacgtcg tccttcactc gctcgccttc gggacgctca
     4681 agccgttcct gggcgacgag aaggagcgga tgacgaaggc gcagatggag atgacgctcg
     4741 acgtcatggc ccactc
//
LOCUS       DAOTEZ010000344         4728 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_13140_length_4728_cov_5.558956, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000344 DAOTEZ010000000
VERSION     DAOTEZ010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13140_length_4728_cov_5.558956"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..729
                     /locus_tag="P5164_15385"
     CDS             40..729
                     /locus_tag="P5164_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY45319.1"
                     /translation="MDAVIATRGLTKVFGLNGNAVHALRGIDLEVRRGEFVALVGPSG
                     SGKSTLMAILGCLDSPTGGTYALDGQAVEGLSGGALARIRNEKVGFVFQTYNLLPKAT
                     VARNVELPMLYAGVGGRERRARALELLEKVGIPEKAGRLPAELSGGQRQRVAIARALA
                     NRPALLLADEPTGALDSKTGAEVLALFRDLHAQGHTVVLVTHDPSIAALAERRVELHD
                     GLIRLPEAKAA"
     gene            731..1981
                     /locus_tag="P5164_15390"
     CDS             731..1981
                     /locus_tag="P5164_15390"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY45320.1"
                     /translation="MRFSGAFRQRVAEAWIEIRENLGRSALQGLGIMVGVASVLGGFA
                     ISDSSQRRSDERWRKLGGIERLNVQPAAVASDGNPTALQRANLGLRNADAVDGEGMNR
                     KAVHATSVQRFARARVRSPYADQERSVSGIGGDFLPLNGYDVEEGRAFSSTELAAGAP
                     VCLLGAEAAAAFFPSGGAVGQTVRVGDVPVVVVGVLRELVFRWRDGEPNAMAWRNRIV
                     AVPAALVASRMNGDRYERVDRVTFKIPELSLMEGFSRDLASLLKANHRQQDDVRIDDI
                     AARIRKRQSQGQVYNMIFMLAGILSLIGGGMVNVNIQLASLGERVREVGVRMAIGASG
                     REVFKSFMTEALLLTGLGSLVGLLLGIAFSKGICVFLDIPLFLAPGSFAAAFFVASAF
                     GFLFALYPAWKASRLSPMEALRYE"
     gene            1974..3272
                     /locus_tag="P5164_15395"
     CDS             1974..3272
                     /locus_tag="P5164_15395"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY45321.1"
                     /translation="MSSTTLGLPLAVAPSRRSRRDSIPARLELGLEVVRGGLLELWSH
                     KLRSILTLTLLMLGVFTLVVLTSVIDGVLDKVSTGFAGMAWDGTAVLQPREPKTTEDQ
                     KRFAMSPGLRFDDLSRLTVPHPKVAAFLPRATKRTTVRVAGGTERVFVTGLTPDYAVF
                     MNRPVSLGRGLTADDARRRSTVAVVGATLASKLFGGADPVGRDVVLEGVPWRIVGVLA
                     PLMVFNEELYYDANGLMVPLETYVVRLDPDRKLTHVAVKLRDKRDLEEVSSLVLSRAR
                     QAHHGIEDTEIKDLDAEAAKAYSQFLEEMRSWRVILSALAGTVLLVGGVGVLSVMLIS
                     YASRRYEIGLRKAMGASDTEILVQFLLEALVLASLGALLGTVGGAFLCQKLSHFFPWG
                     LVVNPVGLLVAWGVALGLALVFGLYPAMRAARLSPMEAMR"
     gene            3329..4306
                     /locus_tag="P5164_15400"
     CDS             3329..4306
                     /locus_tag="P5164_15400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRY45322.1"
                     /translation="MNSLPHSGETIPPELSVVVIVGPLRERAAGCLESLLSQDTVDRL
                     EVLLVDLCSDLPPVEGASHPAVVTLPMPPETSFAEARVTAIRAARGKAVALVEEHSRA
                     LPGWAGAVLAAHEEGWTAVASEMHVANPGIGKADVNGVMNYGLFSPPLARGEGTMAPP
                     FHTSFLRSALLDLGDELPADAFSDICLWEGVRRRGGRLLQEPAARLAHINETSLLEPF
                     LADFHFHRFWVGWRRRTEGWSPARTAAAVLLFPVYPAYYLVLHGRRLRARSPESARVL
                     RRHWLHAWLRQLGPALGQAAGILFGPGRAAARFTRYEIVERRGTANGPL"
     gene            4303..>4728
                     /locus_tag="P5164_15405"
     CDS             4303..>4728
                     /locus_tag="P5164_15405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY45323.1"
                     /translation="MTTRPRVSVVFPAYRSEATVAGCLSALRRQTFTDFEVVLVDSGP
                     GGSTGAIVARDFPEVRCHVSPTRLLPHAARNLGVSMARGELFLLSDPDTYARPDWIER
                     MVSAWERTGGIIVGAIANHGDRWLDTGIHICKFSKVLPGG"
BASE COUNT          615 a         1800 c         1586 g          727 t
ORIGIN      
        1 gacccgcaag aaggtcgaga aggacgagga caactgagca tggacgccgt catcgcgacc
       61 cgcggcctca cgaaggtctt cggcctgaac gggaacgccg tgcacgccct ccgcgggatc
      121 gacctcgagg tgcgtcgcgg ggagttcgtc gcgctcgtcg gcccctccgg ctcggggaag
      181 tcgacgctga tggcgatcct cggctgcctc gactcgccga cgggcggcac ctacgcgctc
      241 gacgggcagg ccgtcgaggg gctctcgggc ggcgcgctcg cgcgcatccg caacgagaag
      301 gtcggcttcg tcttccagac ctacaacctc ctgccgaagg cgaccgtcgc ccgcaacgtg
      361 gagctcccga tgctctatgc cggcgtcggc ggccgcgagc gtcgcgcccg cgccctcgag
      421 ctcctcgaga aggtcgggat cccggagaag gcggggcgcc tccccgccga gctctccggc
      481 ggccagcggc agcgcgtcgc gatcgcccgc gcgctcgcga accgcccggc cctcctcctc
      541 gccgacgagc cgacgggggc cctcgactcg aagaccggcg ccgaggtcct cgccctcttc
      601 cgcgatctcc acgcccaggg gcacaccgtc gtcctcgtga cgcacgaccc ctcgatcgcc
      661 gccctcgccg aaaggcgcgt cgagctccac gacggcctga tccgcctccc cgaggcgaag
      721 gcggcctgac gtgcgtttct ccggcgcctt ccgccagcgg gtcgccgagg cctggatcga
      781 gatccgggag aacctcggcc gctcggccct ccaggggctc ggcatcatgg tcggcgtcgc
      841 ctcggtcctg ggcgggttcg cgatctccga ctcgagccag cggcgctccg acgagcgctg
      901 gcggaagctc ggcggcatcg agcgcctgaa cgtccagccc gcggccgtcg cgagcgacgg
      961 gaacccgacc gccctccagc gggcgaacct cggcctccga aacgccgacg ccgtcgacgg
     1021 cgaggggatg aaccggaagg ccgtccacgc gacgagcgtc cagcgcttcg cccgcgcccg
     1081 cgttcgatct ccgtatgccg accaggagcg ctcggtgagc ggcatcggcg gcgacttcct
     1141 cccgctgaac ggctacgacg tcgaggaggg gcgggccttc tcctcgacgg agctcgccgc
     1201 gggcgccccc gtctgcctcc tcggcgccga ggcggcggcc gcgttcttcc cgtcgggcgg
     1261 cgcggtcggc cagacggtcc gtgtcggcga cgtcccggtc gtcgtcgtcg gcgtcctccg
     1321 cgagctcgtc ttccgctggc gcgacggcga gccgaacgcc atggcctggc ggaaccggat
     1381 cgtcgccgtg ccggccgccc tcgtcgcctc gcggatgaac ggcgaccgct acgagagggt
     1441 cgaccgcgtg acgttcaaga tcccggagct gagcctcatg gagggcttct cccgcgacct
     1501 cgcgtcgctc ctgaaggcga accaccgcca gcaggacgac gtgaggatcg acgacatcgc
     1561 ggcccggatc cgcaagcggc agagccaggg gcaggtctac aacatgatct tcatgctcgc
     1621 cgggatcctc tccctcatcg ggggcgggat ggtcaacgtg aacatccagc tcgcgagcct
     1681 cggcgagagg gtccgcgagg tcggcgtgcg gatggcgatc ggcgcctcgg gccgcgaggt
     1741 cttcaagagc ttcatgacag aagcgctcct cctgacgggc ctcggcagcc tcgtcggcct
     1801 cctcctcggc atcgcgttct cgaaggggat ctgcgtcttc ctcgacatcc cgctcttcct
     1861 cgcgccgggg agcttcgccg cggccttctt cgtggcgagc gccttcggct tcctgttcgc
     1921 cctctacccg gcctggaagg cctcccggct gtcgcccatg gaggcgctgc ggtatgagta
     1981 gcaccaccct cgggctcccc ctggccgtcg cgccgtcccg ccgcagccgg agggactcta
     2041 tccccgcccg cctcgagctc gggctcgagg tcgtccgggg cgggctcctc gagctctggt
     2101 cccacaagct ccgctcgatc ctcacgctca cgctcctgat gctcggcgtc ttcaccctcg
     2161 tcgtcctgac gtccgtcatc gacggggtcc tcgacaaggt ctcgaccggc ttcgccggga
     2221 tggcgtggga cggaacggcc gtcctgcagc cgcgcgagcc gaagacgacg gaggaccaga
     2281 agcggttcgc gatgtcgccg gggctccgct tcgacgacct ctcgcgcctc accgttcctc
     2341 acccgaaggt cgccgcgttc ctcccgcggg cgacgaagcg gacgacggtc cgcgtcgcgg
     2401 gcgggacgga gcgggtcttc gtcaccggcc tgacgcccga ctacgccgtc ttcatgaacc
     2461 gccccgtctc cctcgggcgc ggcctgacgg ccgacgacgc ccggaggagg agcacggtcg
     2521 ccgtcgtcgg cgcgacgctc gcctcgaagc tcttcggcgg ggcggacccc gtcgggaggg
     2581 acgtcgtcct cgagggcgtc ccctggcgga tcgtcggcgt cctggcgccg ctgatggtct
     2641 tcaacgagga gctctactac gacgcgaacg ggctcatggt cccgctcgag acctacgtcg
     2701 tccgcctcga cccggaccgg aagctgacgc acgtcgccgt gaagctccgc gacaagcggg
     2761 acctcgagga ggtctcctcg ctcgtcctct cccgagcgcg ccaggcgcac cacggcatcg
     2821 aggacaccga gatcaaggac ctcgacgcgg aagccgcgaa ggcctactcg cagttcctgg
     2881 aagagatgcg ctcctggcgg gtcatcctct ccgccctcgc ggggaccgtc ctcctcgtcg
     2941 gaggcgtcgg cgtcctctcg gtcatgctca tctcgtacgc atcccggcgg tacgagatcg
     3001 ggctgaggaa ggcgatggga gcctccgaca ccgagatcct cgtccagttc ctcctcgagg
     3061 ccctcgtcct cgcctcgctc ggcgcgctcc tcgggacggt cggcggggcg ttcctctgcc
     3121 agaagctctc tcacttcttc ccgtggggac tcgtcgtgaa cccggtcggc cttctcgtcg
     3181 cctggggcgt cgcgctcggc ctggccctcg tcttcggcct ctatcccgcg atgcgcgcgg
     3241 cgcgcctctc gccgatggag gcgatgaggt agggccgcgg agcctcgcgg cagggccggg
     3301 ataagatccc gccccgctgc gaggctccgt gaactccctt ccccattctg gcgaaacgat
     3361 tcctcccgag ctctccgtcg tcgtcatcgt cggcccgctt agggagcgcg ccgcgggctg
     3421 tctcgagtcc ctgctgtcgc aggacaccgt cgaccggctg gaagttctcc tcgtcgacct
     3481 ctgcagcgac ctgccgcccg tggagggcgc ctctcatccg gccgtcgtca cgctcccgat
     3541 gccgccggag acgagcttcg ccgaggcgcg cgtcaccgcg attcgcgccg cccgcgggaa
     3601 ggccgtcgcg ctcgtcgagg agcacagccg cgccctgccg ggctgggccg gcgccgtcct
     3661 cgccgcccac gaggaagggt ggaccgccgt cgcctccgag atgcacgtgg cgaaccccgg
     3721 aatcggcaag gccgacgtga acggcgtcat gaactacggc ctcttcagcc ccccgctcgc
     3781 gcgcggagaa gggacgatgg cgccgccgtt ccacacgagc ttcctccgtt cggcgctgct
     3841 cgacctcgga gacgagctgc cggcggacgc gttctccgac atctgcctct gggagggagt
     3901 gcgccgccgg ggcggccggc tcctgcagga acccgcggct cggctcgccc acatcaacga
     3961 gacgtcgctc ctcgagccgt tcctcgcgga cttccacttc caccgtttct gggtcggctg
     4021 gcggaggagg acggagggct ggagccctgc ccggacggcc gccgcggtcc tcctcttccc
     4081 cgtctacccg gcctactacc tcgtcctcca cgggcggagg ctccgcgcga ggagcccgga
     4141 atcggcccgg gtcctccgcc gacactggct ccacgcctgg ctccgccagc tcggaccggc
     4201 cctcgggcag gccgccggga tcctcttcgg cccgggccgg gccgcggccc ggttcacccg
     4261 atacgagatc gtcgagcgac gcggcacggc gaacggaccg ttgtgaccac gcgcccccgc
     4321 gtctccgtcg tcttccccgc ctaccgctcc gaagcaaccg tagcgggatg cctttccgcg
     4381 ctccgccggc agacgttcac cgacttcgag gtcgttctcg tcgactccgg gccgggcggg
     4441 tcgaccgggg ccatcgtggc acgcgacttt ccggaggtcc ggtgccacgt ctcaccgact
     4501 cggcttctgc cccacgccgc ccggaacctc ggagtctcga tggcgcgggg agagctcttc
     4561 cttctctccg accccgacac gtatgcccgg ccggactgga tcgagcggat ggtctccgcc
     4621 tgggagcgca ccggcgggat catcgtcgga gcgatcgcga accacgggga ccggtggctc
     4681 gataccggga tccacatctg caagttctcg aaggtcctcc ccggcggc
//
LOCUS       DAOTEZ010000345         4705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_13226_length_4705_cov_5.637849, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000345 DAOTEZ010000000
VERSION     DAOTEZ010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13226_length_4705_cov_5.637849"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="P5164_15410"
     CDS             complement(<1..173)
                     /locus_tag="P5164_15410"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRY45324.1"
                     /translation="MRPIELSIPLGIGTPAWPTYEPLQMKYFKRLAPNGANGQLLTHS
                     NHLGTHMDGEIHF"
     gene            complement(190..1635)
                     /locus_tag="P5164_15415"
     CDS             complement(190..1635)
                     /locus_tag="P5164_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HRY45325.1"
                     /translation="MSATSTSLLDGPIEVVHAGIDAVAEAPRAAGAPVTVVDFRPPAG
                     GEPRLMALLSELLSGPSALEIDAANARALARLLASRPHLVAIGVARDVIPGMADDLFL
                     HAGPPVSWERMCGPMRGAVIGGLLYEGRAKDPAEAERLAASGVVRFEPCHHHDAVGPM
                     AGLVTPSMPVFVIEDRGGERPGARTFCTLNEGLGKVLRYGAFSEEVVARLVFMRDELG
                     PVLAEALARRGPIDLKHLMAQALQMGDEGHNRNRAGTSLLFRELAPAVVRATADRERA
                     ARVLDFVNGNDHFFLNLSMPMGKCMLKAAEGEPLSSLVSVMARNGTDFGIQLASMPGR
                     WFTGPAQMVRGLYFPGRTEADANPDIGDSAITETAGFGGFAMAAAPAIVQFVGGSAED
                     ALAATRAMRRITLGPNPGFAIPALGFSGSPTGIDLRLVEERNLRPQINTGIAHKEPGV
                     GQVGAGLVTPPWEAFHAALEAFAEHVRASRA"
     gene            complement(1632..3167)
                     /gene="fdrA"
                     /locus_tag="P5164_15420"
     CDS             complement(1632..3167)
                     /gene="fdrA"
                     /locus_tag="P5164_15420"
                     /inference="COORDINATES: protein motif:HMM:NF004760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HRY45326.1"
                     /translation="MTTRILVRSGCYHDSARLMQVSRELSALPGIEEAVAMMGTETNR
                     RLLADAGFLGTDVDAATPLDMIVGLRGDPEDAVAAATASLDRLLSGGATSAGGPAAPE
                     RPGTVPEALVARPAANLVSVAVPGPYAAFVAHRALDAGRSVFLFSDNVAVADEIALKR
                     RAASLGLLVMGPDCGTAILAGVGLGFANRVERGPVGIVGASGTGIQELCSLLDRSGVG
                     VSHAIGTGSRDLSAGVGGLMTEAGLGLLAKDPATRALLLVAKHPDAGVAERLHGLLSR
                     LGKPVAVRYLGEGRRGEVDGVLYAGSLDEAAEAAVSRVASTPAVRAGLAPSASDAVAR
                     RGEGEHRLVGLFGGGSLAAEARCVLEGRGIAVRVPEEKLAANGPLPPGNLVVDTGDDA
                     YTVGRPHPMVDQTVRCELIRAAGSDRSVGVLLLDLVLGDGAHPDPAPEIVEAVAAARA
                     ARGSRPLSVVASVCGTRRDPQGTARQEETLRRAGVEVEPSAARATATAADFVLSVRQE
                     VAR"
     gene            complement(3164..3841)
                     /locus_tag="P5164_15425"
     CDS             complement(3164..3841)
                     /locus_tag="P5164_15425"
                     /inference="COORDINATES: protein motif:HMM:NF022834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HRY45327.1"
                     /translation="MRIQDLESLLSPAAATPRPPLSCRDLDPVPGPISRATLVLDLSS
                     LPVPTRTRALLSGEGGAPDSVESRVFRWAHARVGSLVEKLLSGELRVAAYRPDAAGLT
                     GLGPGLTPTGDDLLVGLSAMARRLAGGGLVEIRAAVALSTVLAGLPAEETTPAAHLLL
                     REASEGRFPAVLATVVELIGNPHADAESLRSAGARLVATGAHSGADLLAGALALVQGV
                     VDARESS"
     gene            complement(3838..4452)
                     /locus_tag="P5164_15430"
     CDS             complement(3838..4452)
                     /locus_tag="P5164_15430"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRY45328.1"
                     /translation="MSNTCTPVAAASSYLPEGLLFLDPETSQSAKPVAKWTFGAQAVS
                     IAVDPETGQVTVEKVAACYDVGKVVNPALIKGQTYGGIVQGLGTGTMEELVLDPKTGR
                     AVNASLMDYKIPSTEDVPAEMAVDFVETAQKDGPMGARGIGEHTMIPTPAAIANALWD
                     ACGIRISEMPITAEKVWRALKAKERAERGAKAAGEARVPAGTVA"
     gene            4442..>4705
                     /locus_tag="P5164_15435"
     CDS             4442..>4705
                     /locus_tag="P5164_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45329.1"
                     /translation="MFDTGNASSDSLTGEAMSKRPGWTELAEGDVEHVFKGDAALVND
                     RVACVLRRGSTGAEVYSLGPGRPVMRAVLAPAVRGENAKLISFR"
BASE COUNT          634 a         1691 c         1784 g          596 t
ORIGIN      
        1 tagaagtgga tctcgccgtc catgtgcgtc ccgaggtggt tgctgtgcgt caggagctgg
       61 ccgtttgcgc cgttcggcgc gagccgcttg aagtacttca tctggagcgg ctcgtaggtc
      121 ggccaggccg gggtgccgat gccgaggggg atcgagagct cgatcgggcg catggtgggc
      181 tccttcgttt caggcgcggg aggcgcggac gtgttcggcg aatgcctcga gggcggcgtg
      241 gaacgcctcc cacggggggg tgacgagacc ggctcccacc tgcccgacgc cgggctcctt
      301 gtgggcgatg ccggtgttga tctgcggtcg gaggttccgc tcctccacga ggcggaggtc
      361 gatgccggtc ggcgagccgg agaatccgag cgccgggatc gcgaagccgg ggttcggccc
      421 gagcgtgatc cggcgcatgg cgcgcgtcgc cgcgagcgcg tcctcggcgc tcccgccgac
      481 gaactggacg atcgccgggg cggcggccat ggcgaaaccg ccgaaaccgg ccgtctcggt
      541 gatcgcgctg tcgccgatgt cggggttcgc gtcggcttcc gtgcggcccg ggaagtagag
      601 gccccgcacc atctgcgccg gcccggtgaa ccacctgccc ggcatcgagg cgagctggat
      661 tccgaagtcg gtgccgttgc gggccatgac ggagacgagc gaggagagcg gctccccttc
      721 cgccgccttc agcatgcact tgcccatcgg catcgagagg ttcaggaaga agtggtcgtt
      781 gccgttcacg aagtcgagga cccgcgcggc acgctcccgg tccgcggtgg cccggacgac
      841 ggccggcgcc agctcgcgga agagaagcga cgtccccgcg cggttccggt tgtgcccctc
      901 gtcccccatc tggagcgcct gggccatcag gtgcttcagg tcgatcggac ctcggcgcgc
      961 gagcgcctcc gcgaggacgg ggccgagctc gtcccgcatg aagacgagcc gcgcgacgac
     1021 ctcctccgag aaggcgccgt aacggaggac ctttccgagg ccctcgttca gcgtgcagaa
     1081 cgtccgcgct cccgggcgct cgccgccgcg gtcctcgatc acgaaaaccg gcatcgacgg
     1141 cgtgacgagg cccgccatcg ggccgacggc gtcgtggtgg tggcacggct cgaagcgaac
     1201 gacgccgctc gccgcgagac gctccgcctc ggccgggtcc ttcgcgcgcc cctcgtagag
     1261 gagtccgccg atcacggcac cccgcatcgg cccgcacatc cgctcccacg acacgggagg
     1321 tccggcgtgg aggaagaggt cgtccgccat ccccgggatc acgtcgcgcg cgacgccgat
     1381 cgcgacgagg tgcgggcggc tcgcgagaag ccgggcgagg gcgcgggcgt tcgcggcgtc
     1441 gatctcgagc gcggaggggc cggagaggag ctccgacagg agcgccatga ggcgcggttc
     1501 gcctcccgcc ggaggccgga agtcgacgac cgtgacgggt gcgccggcgg cgcggggcgc
     1561 ttcggcgacg gcgtcgatgc cggcgtggac gacctcgatc gggccgtcga ggagagaggt
     1621 cgacgtcgcg ctcaccgggc cacctcctga cggacggaga ggacgaagtc cgcggccgtc
     1681 gccgtcgcgc gggcggcgga aggctccacc tcgacgcccg cgcgccggag cgtctcctcc
     1741 tgccgggccg tcccctgcgg gtcccggcgc gtgccgcaga cggaggcgac cacggagagc
     1801 ggccgggagc cccgcgcggc gcgcgccgcg gcgacggctt cgacgatctc gggcgccggg
     1861 tcggggtggg cgccgtcgcc gaggacgagg tcgaggagga ggacgccgac ggaacggtcc
     1921 gaccccgcgg cgcggatcag ctcgcagcgg acggtctggt cgaccatcgg gtgcggcctt
     1981 ccgaccgtgt aggcgtcgtc ccccgtgtcg acgacgaggt tcccgggcgg gagcggcccg
     2041 ttcgccgcga gcttctcctc cggcacgcgc acggcgatcc cccgcccttc gaggacgcag
     2101 cgcgcctcgg ccgcgagaga gccgccgccg aagaggccga cgagccggtg ctcgccctct
     2161 cctcgccgcg cgaccgcgtc gctcgccgag ggcgcgaggc cggcccggac ggccggcgtc
     2221 gacgcgacgc gagagaccgc cgcctcggcc gcctcgtcga gcgatcccgc gtagaggacg
     2281 ccgtccacct ctccgcggcg tccctcgccg aggtagcgga cggcaaccgg cttgccgagc
     2341 ctcgagagga gcccgtgaag gcgctccgcg acgcccgcgt ccgggtgctt ggcgacgagg
     2401 aggagggcgc gcgtcgccgg gtccttcgcg aggaggccga gcccggcctc cgtcatcagg
     2461 ccgcccaccc cggcggagag gtcgcggctc ccggtgccga tggcgtgcga gacgccgacg
     2521 ccgctccggt cgaggaggga gcagagctcc tggatcccgg tgcccgaggc gccgacgatc
     2581 ccgaccggcc cgcgctccac gcggttcgcg aagccgagcc cgacgccggc gaggatcgcc
     2641 gtgccgcagt cgggtcccat gacgaggagg ccgagcgagg ccgcccgccg cttcagggcg
     2701 atctcgtccg cgaccgcgac gttgtcggag aagaggaaga cgctccgccc cgcgtccagc
     2761 gcccggtgcg cgacgaacgc cgcatacgga ccgggaaccg cgacggagac gaggttcgcc
     2821 gcggggcgcg ccacgagcgc ctcggggacc gtcccgggcc gctccggagc cgccggcccg
     2881 ccggcgctcg tcgcgccgcc ggaaaggagc cggtcgagag acgccgtggc cgccgcgacg
     2941 gcgtcctccg gatctccgcg aaggccgacg atcatgtcga gcggagtcgc ggcgtcgacg
     3001 tccgtcccga ggaagccggc atcggcgagg agacggcgat tcgtctccgt ccccatcatc
     3061 gcaaccgctt cctcgatgcc gggaagcgcg gagagctcgc ggctcacctg catgagccgc
     3121 gcggagtcgt ggtagcagcc ggagcggacg aggatgcgtg tcgtcatgag ctctcccggg
     3181 cgtcgacgac gccctggacg agggcgagcg ctccggcgag gaggtccgcc cccgagtgcg
     3241 cgcccgtcgc gacgaggcgc gcgccggccg aacggagcga ctccgcgtcg gcgtgggggt
     3301 tcccgatcag ctccacgacc gtcgcgagga ccgccgggaa gcgtccctcg gaggcctccc
     3361 gaaggaggag gtgcgccgcg ggcgtcgtct cctccgccgg cagcccggcg aggaccgtgg
     3421 agagggcgac ggcggcgcgg atctcgacga ggcctccgcc cgcgaggcgg cgcgccatcg
     3481 ccgagagccc gacgagcagg tcgtcccccg tcggcgtcag gcccggcccg agaccggtca
     3541 ggccggccgc gtcgggacgg taggcggcga cgcggagctc ccccgagagg agcttctcga
     3601 cgagcgaccc gacccgggca tgcgcccagc gaaagacccg gctttcgacg gagtcgggcg
     3661 cgccgccctc gcccgagagg agcgcccgcg tgcgcgtcgg gacagggagg gaggagaggt
     3721 cgaggacgag cgtcgcccgg gagatcggcc ccggcaccgg gtcgaggtcg cgacagctga
     3781 gcggaggccg cggcgtcgcc gccgccggag acaggagcga ctcgaggtcc tggatcctca
     3841 cgcgaccgtc cccgccggga cccgagcctc gcccgcggcc ttcgcgccgc gctcggcccg
     3901 ttccttcgcc ttcagggccc gccagacctt ctccgccgtg atcggcatct cggagatccg
     3961 gatgccgcag gcgtcccaga gcgcgttcgc gatcgcggcc ggcgtcggga tcatcgtgtg
     4021 ctcgccgatg ccgcgcgccc ccatcggccc gtccttctgc gccgtctcga cgaagtcgac
     4081 ggccatctcg gccgggacgt cctccgtcga cgggatcttg tagtccatga gcgaggcgtt
     4141 cacggcgcgg cccgtcttcg ggtcgaggac gagctcctcc atcgtgccgg tgccgagccc
     4201 ctggacgatg ccgccgtagg tctgcccctt gatgagcgcc gggttcacga ctttcccgac
     4261 gtcgtagcag gccgcgacct tctccaccgt cacctgcccc gtctccgggt cgaccgcgat
     4321 cgagaccgcc tgcgccccga acgtccactt cgccacgggc ttggcgctct ggctcgtctc
     4381 ggggtcgagg aagaggagcc cctcggggag gtacgaggaa gccgcggcga ccggcgtaca
     4441 cgtgttcgac accggcaacg cttcctctga ttcgctgacc ggtgaagcga tgtcgaagcg
     4501 acccggctgg acggaactcg ccgagggcga tgtcgagcat gtgttcaagg gcgacgccgc
     4561 gctggtgaac gaccgggtag cgtgcgtcct tcgccgaggc tcgacgggcg ccgaggtgta
     4621 ctcgctgggg cccggccggc ccgtgatgcg ggcggtgctg gctccggccg tccgcggcga
     4681 gaacgcgaag ctgatttcat tcaga
//
LOCUS       DAOTEZ010000346         4703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_13238_length_4703_cov_3.727410, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000346 DAOTEZ010000000
VERSION     DAOTEZ010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13238_length_4703_cov_3.727410"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..370
                     /locus_tag="P5164_15440"
     CDS             179..370
                     /locus_tag="P5164_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45330.1"
                     /translation="MQMTDYLLAPVVARKLGVKTATLAKWRRTDRGPRGWVRYSETSV
                     AYPVAEVERWLAERGGEAA"
     gene            367..1491
                     /locus_tag="P5164_15445"
     CDS             367..1491
                     /locus_tag="P5164_15445"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HRY45331.1"
                     /translation="MKTPGLRKGRKAGEWHLEVCVPGTAGRVRKRRTIYADSRAEALH
                     EWSVWRKEIVARGGRSKAWTLRAYVEAHAEAFEARYTTHGARDFAFRCSVLIRELGDI
                     RLDRITAATVRDLAARLQRAYKPSTVNTMLTGLRRLLRDAVDRSELAAYPIRGRLPLA
                     RVEPLRLEMTDEERAAFLAGFDDAARFRRVTETAGPRSDVDFSFHRAMRPVYVVALET
                     GLSRGDLLSLTWADVAGPVIRRPRGKTHVEAVIPVSAACRAALDELKARPIVGKRVFV
                     SPRSGRPLAIDTLQIHFRLAKRIAGITRRLRFHDLRHTFASRLASAGVSLQVIARALG
                     HSSIAMSQRYARPDDAAMRAVLDALTNVPTNAPARSNGGK"
     gene            1492..1818
                     /locus_tag="P5164_15450"
     CDS             1492..1818
                     /locus_tag="P5164_15450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45332.1"
                     /translation="MGPDGYNLDHLTSTRVESHPGTAPDTAEQPNQAGSRSDDGKPGA
                     SSSAATNSARTPDCPACAALREQNRLLSEHNLVLITRVRAFEDRDRLGAEMLLGLAGL
                     QKAAGA"
     gene            1815..3551
                     /locus_tag="P5164_15455"
     CDS             1815..3551
                     /locus_tag="P5164_15455"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRY45333.1"
                     /translation="MTPTLRDYQGREGALGAVEKGLAAGCRRQLVSLATGLGKTVVFA
                     NLPMIGIRRFLVLAHREELLDQAADKLRHWNPGLTVQVDQADRWADVDTLSAFDGALF
                     EGTGYAVCASVATIGRAGSRRLTRYPDGLFDAVVVDECHHATAKSYQSVIDHFHAGRP
                     GGPLLLGFTATPGRADAADLTKVFDKVIYSMDLLEGINRGFLVDVRAIAVSTGVSLDA
                     VGATREDFKTSELADAVNTSERNGRVVKAYLDHAADRKGIVFAADVAHSKALAEAFEA
                     AGVDARHVDGETPSDERAAAFAWLREAGPKVLVNVGIATEGFDEPSVSVILLARPTKS
                     WILYTQMLGRGTRLCDGKVDLLVIDLADLSSKHTLVGAAALFGLPTGLDLAGKSATKA
                     RARVKELVQEGLPFEELAKAKTLADLETIANEYKKVSLFWQPKTAPEVAGVSALSWVS
                     VGDGAIALRAGELRAWITTDMLGHAHLALRRRPVPAPGGLQLPETLELAADFPTLEKA
                     VGAADALITAEMPKVFTDPKARWRKDPASEKQVALLKKFRVPYREPLTKGEAGILLDK
                     VFARRGAVRRSA"
     gene            3548..3952
                     /locus_tag="P5164_15460"
     CDS             3548..3952
                     /locus_tag="P5164_15460"
                     /inference="COORDINATES: protein motif:HMM:NF012974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HRY45334.1"
                     /translation="MSIAFDWAEKPGNVFTSDRAERALLGALLLTGESGLARCERLRP
                     DDFRSPRGAAVLTAIRKLTFLGVTVDALTVVDELERGKVPPPPAGPGWGTAIAALLDH
                     GTVGACDDDSIGAYAQIVVDAAALRRRSAWGA"
     gene            3952..>4703
                     /locus_tag="P5164_15465"
     CDS             3952..>4703
                     /locus_tag="P5164_15465"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HRY45335.1"
                     /translation="MGELHVLTPELLGPQDEVLKVLREDEAKLLGKLALCRGEIARRT
                     GEGNRSTWERPADVLRRQKDVSSRYPTGVPTVDYLAKSGLPRGRIMVMVGRPGAGKTG
                     LAIQIAAFAAEHEPNLVVGLYLADEGLEAGVIRMAQHYGYDREKLEAAELETIRPAAE
                     ALDLLQNVHCMDPDAEDASLEAFLDGMATRADGRPQLWVIDSAQVVRLASAASKKLDR
                     RLTVDSVCRMVKAAARRRGAVALLLSQSNRGA"
BASE COUNT          702 a         1708 c         1614 g          679 t
ORIGIN      
        1 gctcgtctca acgccgcgcc gcttgactgg ccggtccgat acaacgggcg caagcgccgc
       61 atcctccccg gctgcagaga cgggggacgc gggtgaggcg cctcctcgcg tccgccgccc
      121 tcgccaccgt cgcagggctc gggattgggc tggcgctggt cgtcgtgtgg gaggccgcgt
      181 gcagatgacc gactacctcc tcgcccctgt cgtcgcccgc aagctcggcg tgaagaccgc
      241 cacgctcgcc aagtggcgcc gcaccgatcg cgggccgcgc gggtgggtcc gctactccga
      301 gacgtccgtc gcctatccgg tcgccgaggt cgagcggtgg ctggcagagc gcggagggga
      361 ggccgcgtga agacgcccgg tctccgcaag ggccgcaagg ctggcgagtg gcatctggag
      421 gtctgcgtac ccggcacggc cggacgggtc cgcaagcgcc ggacgatcta cgccgactcc
      481 agagcggagg ctctgcacga gtggtccgtc tggcgaaagg agatcgtcgc tcgcggcgga
      541 cgctcgaagg cgtggacgct ccgggcctac gtcgaggcgc acgccgaggc attcgaggcc
      601 cgctacacga cgcacggagc gcgcgacttc gcgttccgtt gctcggtcct gatccgcgag
      661 ctcggggaca tccgcctcga ccggatcacc gcagccacgg tgcgcgacct cgctgctcgc
      721 ctccagcggg cctacaagcc atcgacggtc aacaccatgc tgacgggcct ccgccggctc
      781 ctgcgggacg cggtggaccg ctcggagctg gcggcgtacc cgatacgcgg gaggctcccg
      841 ctcgcccgcg tcgagccgct ccggctggag atgaccgacg aggagcgcgc tgccttcctc
      901 gccgggttcg acgacgcggc tcggttccgg cgggtgaccg agacggccgg gccgaggtcg
      961 gacgtggact tctcgttcca ccgagccatg cgccccgtct acgtcgtcgc gctggagacg
     1021 gggctgtccc gtggcgatct gctctcactg acctgggccg acgtcgccgg gccggtgatc
     1081 cgccggccgc gcgggaagac tcacgtcgag gcggtgatcc cggtctcggc tgcctgccgc
     1141 gccgccctgg acgaactcaa ggcgcggccg atcgtcggca agcgggtctt cgtctcgccc
     1201 cgctcggggc gtcctctggc gatcgacacg ttacagatcc acttccgtct ggcgaagcgg
     1261 atcgccggca tcacccgacg gctgcggttc cacgatcttc gtcacacctt cgcctcccgg
     1321 ctggcgtccg ccggggtctc cctccaggtc atcgcccgcg ctctcgggca ctcctctatc
     1381 gctatgtcgc agcggtacgc ccggccggac gacgcggcga tgagggcggt cctcgatgcc
     1441 ttgacgaacg ttcccacgaa cgcaccggcc cgatccaacg gaggaaagtg aatggggcca
     1501 gacggttaca atctcgacca tctcacctct acgagagtag agagccatcc cggcacggct
     1561 ccggatactg ccgaacagcc taatcaagcg gggtctcgga gcgacgacgg caagccgggg
     1621 gcgtcttcga gcgccgctac gaactcggca cgaactcccg actgccccgc ctgcgccgcc
     1681 ctccgcgagc agaaccgcct cctctccgag cacaacctcg tcctgatcac ccgggtccgg
     1741 gccttcgagg accgcgatcg tctcggggcg gagatgctcc tcgggctcgc ggggctccag
     1801 aaggcggccg gcgcgtgacg cccaccctcc gcgattacca gggccgcgag ggtgccctcg
     1861 gggccgtcga gaagggcctc gccgcgggat gccgccgcca gctcgtctcc ctggcgacgg
     1921 ggttggggaa aacggtcgtc ttcgccaacc tgcccatgat cggcatcagg cgcttcctcg
     1981 tcctcgcgca tcgcgaggag ctgctcgacc aggccgccga caagctccgc cactggaacc
     2041 ccggtctcac ggtccaggtg gaccaggcgg accggtgggc cgacgtcgac acactgtcgg
     2101 cattcgacgg ggcgctcttc gagggcaccg gctacgccgt ctgcgcctcc gtggcaacca
     2161 tcggccgggc gggcagccgg aggctcacgc gctacccgga cggcctcttc gacgccgtcg
     2221 tcgtggacga gtgccaccac gccaccgcga agtcctacca gtcggtcatc gaccacttcc
     2281 atgctgggcg gccgggcggg ccgctgctcc tggggttcac tgcgaccccc gggcgcgcgg
     2341 acgcggcgga cctcacgaag gtcttcgaca aggtcatcta ttcgatggac ctcctggaag
     2401 ggatcaaccg cggtttcttg gtggacgtcc gggccatcgc cgtatcgacg ggcgtcagcc
     2461 tcgacgccgt cggggcgacc cgcgaggact tcaagaccag cgagctggcc gacgcggtga
     2521 acacctcgga gcggaacggc cgggtcgtga aggcctacct cgaccatgca gcggaccgga
     2581 aggggatcgt cttcgcggcc gacgtggcgc actcgaaggc cctggccgag gccttcgagg
     2641 cggccggcgt agacgcccgc cacgtggatg gggagacccc cagcgacgag cgcgcggcag
     2701 cgttcgcctg gctgcgcgag gctggtccga aggtcctcgt caacgtcggc atcgcaaccg
     2761 agggtttcga cgagccgtcg gtatcggtga tcctgctcgc tcggccgacg aaaagctgga
     2821 tcctctacac gcagatgctc gggagaggca cccgcctctg cgacgggaag gtcgacctcc
     2881 tcgtcatcga cctggccgac ctctcctcga agcacacgct cgtcggggcc gcggcgctgt
     2941 tcggcctgcc gaccggtctc gacctcgccg gcaagtccgc gacgaaggcc agggcccggg
     3001 tcaaggagct cgtccaggag ggcctcccgt tcgaggagct cgccaaggcg aagacgctcg
     3061 ccgacctcga gacgatcgcg aacgagtaca agaaggtctc cctcttctgg cagccgaaga
     3121 cggcgccgga ggtcgctgga gtctccgccc tctcgtgggt ctccgtcgga gacggggcga
     3181 tcgcactgcg cgcgggcgag ctccgcgcgt ggatcacgac ggacatgctg gggcacgccc
     3241 acctggcgct ccgccgccgg cccgtacccg cgccaggtgg cctccagctg cccgagaccc
     3301 tcgagctcgc cgccgacttc ccgacgctcg agaaggccgt cggggcggcc gacgccctca
     3361 tcaccgccga gatgccgaag gtgttcaccg acccgaaggc tcgctggcgg aaggacccgg
     3421 ccagcgagaa gcaggtcgcg ctcctgaaga agttccgggt cccgtaccgg gagccgctca
     3481 ctaagggcga ggcggggatc ctgctcgaca aggtgttcgc gcggcgcggg gccgtcagga
     3541 ggtcggcgtg agcatcgcat ttgactgggc cgagaagccc ggcaacgtct tcacgtccga
     3601 tcgcgcggag agagcactgc tcggtgcgct cctcctcaca ggtgagtccg gtctcgcccg
     3661 ctgcgagcgt ctacgtcccg acgatttccg gtcgccgcgc ggcgcagccg tcctgaccgc
     3721 catccggaaa ctcacctttc tcggcgtgac ggtcgacgct ctcaccgtcg tcgacgagct
     3781 cgagcgcggg aaggtgccgc cacctccggc tggcccaggc tggggcaccg ccatcgccgc
     3841 gcttctcgat cacggaacgg tcggggcctg tgacgacgat tcgatcggcg cctacgccca
     3901 aatcgtcgtc gacgccgcgg cactccgccg gcgttccgcg tggggggcct gatgggggag
     3961 ctccacgtcc tcaccccaga gctcctcggg ccgcaggacg aggtgctgaa ggtccttcgc
     4021 gaagacgaag cgaagctcct cgggaagctg gctctctgcc gcggcgagat cgcccgcagg
     4081 acgggcgagg ggaaccggtc gacctgggag cgccctgccg acgtcctgcg ccgccagaag
     4141 gacgtttcct cgcgctaccc gacgggagtc ccgaccgtcg actacctcgc gaagagcggc
     4201 ctaccccgcg gccgaatcat ggtcatggtg ggccgaccag gcgcggggaa gacaggcctc
     4261 gcgattcaga tcgccgcctt cgccgcggag cacgagccga acctcgtcgt cggcctctac
     4321 ctcgccgacg agggcctcga ggccggcgtc atccggatgg cgcagcacta cggctacgac
     4381 cgcgagaagc tcgaggcggc cgagctcgag acgatccgac cagccgcgga ggccctcgac
     4441 ctccttcaga acgtccactg catggacccg gacgccgagg acgcatccct cgaggccttc
     4501 ctcgacggca tggcgacccg ggcggacggc cgcccccagc tctgggtgat cgactccgcc
     4561 caggtcgtca ggctcgcttc tgcggcctcc aagaagctcg accgccgcct gaccgtcgac
     4621 tccgtctgcc gcatggtgaa ggccgccgcg cgccgccggg gggccgttgc gctcctgctc
     4681 tcgcagagca accgcggggc ata
//
LOCUS       DAOTEZ010000347         4659 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_13405_length_4659_cov_5.053432, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000347 DAOTEZ010000000
VERSION     DAOTEZ010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13405_length_4659_cov_5.053432"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="P5164_15470"
     CDS             complement(<1..701)
                     /locus_tag="P5164_15470"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HRY45336.1"
                     /translation="MARLKRGLFMTHTELIARVGDAIKARFSPDPKALEALEEALLAA
                     DVGPATASELVEAVRVEAGKRDAGEADVVRRVLKAEIERRLSVAGPATAAVAAGVPRV
                     VLMVGVNGTGKTTTAAKLAARAAAAGGRPVLAAADTFRAAAIDQLEVWAGRIGIPLVK
                     HRPGADPAAVVFDACAAAKARGADLLLVDTAGRLHTKHNLMEELSKIRRIAGREIPGA
                     PHEVLLVLDAVTGMNG"
     gene            complement(744..1094)
                     /locus_tag="P5164_15475"
     CDS             complement(744..1094)
                     /locus_tag="P5164_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45337.1"
                     /translation="MSFEDLLRPVLAAPGARAVAFLDPQGEAIASLGDHELLETIGAY
                     QSVWTTELSRAAMGGGLGEVAEIDFDFDAGRVLATAVRDGYFLLALFGRDGVPSAARP
                     HLAAARTRLAAEIG"
     gene            complement(1091..1885)
                     /locus_tag="P5164_15480"
     CDS             complement(1091..1885)
                     /locus_tag="P5164_15480"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY45338.1"
                     /translation="MRASRAAVALSSILVLGGCRSTLDAAKKLPEPSPAVADLVDYRQ
                     GLTLLREGRVDEAIQQLGSARAAYPRSAEVANALGLAYLYRKDHRNATKLFAEALALD
                     ENLVEAKNNRGVAAMEAGRFEDAEKDFSEVLAGTRTSEHVKARFNLGLLRARQLRWAD
                     AERELTTVLADDPGYLNAARERGLARMKLEDFRGALEDFLLFLREDGNDPVANYNAAL
                     CLLTTDRRDLAVRYMERTVQAAPESDEARKARRFLGGEPGAAGRER"
     gene            complement(1882..3633)
                     /locus_tag="P5164_15485"
     CDS             complement(1882..3633)
                     /locus_tag="P5164_15485"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRY45339.1"
                     /translation="MAVLPRRPRLRIVTALIATLVVVSIVPLALSAVRLVSINRDALE
                     TAEAQYLTRSAVTLSDGIDAYLRRMLVQVKKIGDGLELARAVAPGEDTFAFVGRTRLI
                     GRYRDAEPSFLVLRALDPDRRGGVVQPAGLDPSLEKELDRAFDAALRGAVYVGDALRV
                     PGIPDPGLVMAAPVPGPDGEAVGVVEAFVSLAPIRSLLEQERKRDLVAYVVDRRGSLV
                     LSSEPAALGGGRPLLDVELVAEFVQHPVRLTRTYSRGEGEAARRVLGTVAPVGPAEDL
                     PDWGVLVEKDVARAFAAATQMTRTSWLVAALAVALAVVAAILAANALSRPVRALAESV
                     HRMSEGDLSQRVEVPGTYELAELAEGFNHMGGELEKSIEKLKLAARENQELFLNSIRA
                     LAAAVDAKDPYTRGHSERVARYSVAIARHLGQPVEEVRKVRIAALLHDVGKIGIDDRI
                     LRKPTALTDDEFEIMKLHPVKGALIMGQIPQLKWIIPGMKYHHEKWDGTGYPDGLKGE
                     EIPMLARIISVADTFDAMTTTRPYQKAMRSDYVVSRIKTFAGTRYDTSVIEALEEALL
                     KNDLEVIGEAARMAEAS"
     gene            complement(3643..>4659)
                     /locus_tag="P5164_15490"
     CDS             complement(3643..>4659)
                     /locus_tag="P5164_15490"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HRY45340.1"
                     /translation="LERLAGALPRIAPGRWLVVSSAPVLAAQGERLRAALARARNLDP
                     DPLLVPDGERAKSWTELGRLLSGMAARGLARDGGIVAFGGGTVGDATGLAASLALRGV
                     PVVQVPTTLLAAADSALGGKTAVNLAAGKNLAGTFHHPRFVCVDTRLLDTLSERDFRS
                     GLAEVAKCALLSVRFWRRLPRLVEGLGSRAPHPLAEAIASSLRLKASIVALDPDEARG
                     LRHVLNLGHTVAHALEGASGYRLRHGEAVAWGLLAALRLSTARGRLPEPLSKDADATL
                     RELLAPPPLSPGVRRGFPRFLPLDKKRDRAGLRDVLLDAPGRPRLARVTEEELAASLP
                     SGDG"
BASE COUNT          622 a         1702 c         1714 g          621 t
ORIGIN      
        1 ccgttcatcc ccgtcaccgc atcgaggacg agcaggacct cgtggggcgc ccccgggatc
       61 tcccgtccgg cgatccgccg gatcttggag agctcctcca tgaggttgtg cttcgtgtgg
      121 aggcgtcccg ccgtgtcgac gaggaggagg tcggcgccgc gcgccttcgc cgcggcgcac
      181 gcgtcgaaga cgacggcggc cgggtcggcg cccggtcggt gcttcacgag cggaatgccg
      241 attcgtccgg cccagacctc gagctggtcg atcgcggccg cccggaaggt gtcggcggcg
      301 gcgaggaccg gccgcccccc ggccgcggcg gcgcgcgcgg cgagcttcgc cgccgtcgtc
      361 gtcttccccg tcccgttcac gccgaccatc aggacgacgc gcggcacccc ggccgccacc
      421 gcagccgtcg cgggacccgc caccgagagc cgccgctcga tctcggcctt caggacgcgg
      481 cggacgacgt ccgcctcgcc cgcgtcgcgc ttccccgcct cgacccggac cgcctcgacg
      541 agctcggagg cggtcgccgg tccgacgtcc gccgcgagga gcgcctcctc gagcgcctcg
      601 agcgccttcg gatcgggcga gaagcgggcc ttgatcgcgt cgccgacgcg cgcgatcagc
      661 tccgtgtggg tcatgaagag cccgcgcttc aggcgggcca tcagcgacgt gcgagctccc
      721 tcggccacgg tcccctgccc ccttcagccg atctcggcag cgagccgcgt gcgggcggcg
      781 gccaggtgcg gacgcgcggc ggacgggacg ccgtcccgcc cgaacagggc gaggaggaag
      841 tagccgtccc ggaccgcggt cgcgagcacc cgccccgcgt cgaagtcgaa gtcgatctcg
      901 gcgacctcgc ccaggccgcc gcccatcgcg gcccgggaga gctccgtcgt ccagacggac
      961 tggtaggcgc cgatcgtctc gagcagctcg tggtcgccga gggaggcgat cgcctcgccc
     1021 tgcgggtcga ggaacgcgac cgcccgcgcg ccgggcgcgg cgaggaccgg acggaggagg
     1081 tcttcgaagc tcatcgttcc ctgcctgccg ctcccggctc gccgccgagg aagcggcgcg
     1141 ccttcctcgc ctcgtcgctc tccggcgcgg cctggaccgt tcgctccatg tagcggacgg
     1201 cgaggtcccg acggtcggtc gtcaggaggc agagggcggc gttgtagttg gcgaccgggt
     1261 cgttcccgtc ctcgcggagg aagaggagga agtcctccag cgcgccgcga aagtcctcga
     1321 gcttcatcct cgcgaggccc cgctcgcgcg cggcgttgag gtagcccggg tcgtcggcga
     1381 ggacggtcgt cagctcgcgc tccgcgtcgg cccagcggag ctgccgcgcc cgcaggagcc
     1441 ccaggttgaa tcgcgccttc acgtgctcgg acgtacgcgt cccggccagg acctcggaga
     1501 agtccttctc ggcgtcctcg aatcgacccg cctccatcgc cgcgacgccc cggttgttct
     1561 tcgcctcgac gaggttctcg tccagcgcga gcgcctccgc gaagagcttc gtcgcgttcc
     1621 ggtggtcctt ccggtagagg tacgccagcc cgagcgcgtt cgccacctcg gcgctccgcg
     1681 gataggccgc ccgggccgag ccgagctgct ggatcgcttc gtcgacgcgc ccctcccgca
     1741 agagggtgag cccctggcgg tagtcgacga ggtcggcgac ggccgggctc ggctccggaa
     1801 gcttcttcgc ggcgtcgagg gtgctccggc acccgcccag gacgaggatc gatgagagcg
     1861 ccacggcggc gcgggaggcc ctcacgacgc ctccgccatc cgcgccgcct ccccgatcac
     1921 ctcaaggtcg ttcttcagga gggcctcctc gagcgcctcg atgacgctcg tgtcgtagcg
     1981 ggtgccggcg aacgtcttga tgcgggagac cacgtagtcg ctcctcatcg ccttctggta
     2041 ggggcgggtc gtcgtcatcg cgtcgaacgt gtcggccacc gagatgatcc tcgcgagcat
     2101 cgggatctcc tctcccttca gcccgtcggg atatccggtc ccgtcccact tctcatggtg
     2161 gtacttcatc ccgggaatga tccacttcag ctgggggatc tgccccatta tgagcgcccc
     2221 cttgaccggg tgcagcttca tgatctcgaa ctcgtcgtcc gtgagcgccg tcggcttgcg
     2281 caggatccgg tcgtcgatcc cgatcttccc gacgtcgtgg aggagcgcgg cgatccggac
     2341 cttccggacc tcctcgaccg gctggccgag gtgccgggcg atcgcgacgg agtagcgcgc
     2401 gacccgctcc gagtggccgc gcgtgtacgg gtccttcgcg tcgacggcgg cggcgagcgc
     2461 ccggatcgag ttgaggaaga gctcctggtt ctcacgggcg gcgagcttca gcttctcgat
     2521 cgacttctcg agctcgcccc ccatgtggtt gaacccttcg gcgagctccg cgagctcgta
     2581 cgtcccgggc acctcgacgc gctgcgagag gtccccttcg gacatccggt ggacggactc
     2641 cgcgagcgcc cggacgggcc tcgagagcgc gttcgcggcg aggatcgccg cgacgacggc
     2701 gagggcgacg gcgagcgccg cgacgagcca cgacgtccgc gtcatctgcg tcgccgccgc
     2761 gaacgcccgg gcgacgtcct tctcgacgag gactccccag tcgggcagat cctcggccgg
     2821 accgaccgga gcgaccgtcc cgaggacccg ccgtgcggcc tctccctcgc cccgcgaata
     2881 ggtccgcgtc agccggaccg gatgctggac gaactcggcg acgagctcca cgtcgaggag
     2941 cggcctgccg cccccgaggg ccgccggctc gctcgagagg acgaggctcc ccctccggtc
     3001 aacgacgtag gcgacgaggt cgcgcttgcg ctcctgctcg aggagcgacc ggatcggcgc
     3061 gagcgagacg aacgcctcga cgaccccgac ggcctccccg tccggcccgg ggacgggcgc
     3121 ggccatgacc aggcccggat cggggatccc aggaacgcgg agggcgtcgc cgacgtagac
     3181 ggcgccgcgc agcgcggcgt cgaaggcccg gtcgagctcc ttctcgaggg acgggtcgag
     3241 gcccgcgggc tgcacgactc cgccgcgccg gtcggggtcg agagccctga ggacgaggaa
     3301 gctcggctcc gcgtcgcggt aacggccgat gaggcgcgtc ctcccgacga aggcgaaggt
     3361 gtcctcgccg ggggcgacgg cccgggcgag ctccagcccg tcgccgatct tcttcacctg
     3421 gacgagcatt cgtctcaggt aggcgtcgat cccgtcggag agcgtgacgg cgctccgggt
     3481 caggtactgc gcctccgcgg tctcgagggc gtcgcggttg atggagacga gccggaccgc
     3541 agaaagcgcg agggggacga tcgagacgac gacgagcgtc gcgatcagcg ccgtgacgat
     3601 gcgcagccgg ggtcggcgag gaaggacggc caccgggaga ttctagccgt cgccgctcgg
     3661 aaggctcgcg gcgagctcct cctccgtcac gcgcgcgagg cgaggccgcc cgggggcgtc
     3721 cagcaggacg tcccggaggc cggcgcggtc ccttttcttg tcgagcggga ggaacctcgg
     3781 gaagccccgg cggacgcccg gcgaaagggg cggcggcgcg aggagctcgc ggagcgtcgc
     3841 gtcggcatcc ttcgagagcg gctcgggcag ccggccccgg gccgtggaaa gtcgaagggc
     3901 ggcgagaagc ccccaggcga ccgcctcgcc atgccggagc cggtagccgg acgccccctc
     3961 gagcgcgtgg gcgaccgtat gcccgaggtt caggacgtgg cgcaggccgc gggcctcgtc
     4021 ggggtcgagg gcgacgatcg acgccttcag ccggagcgag ctcgcgatcg cttccgcgag
     4081 cgggtgcgga gcgcgcgagc cgaggccttc gacgaggcgc ggcaggcgcc gccagaaccg
     4141 gacggagagg agcgcgcact tggcgacctc cgcgagcccc gagcggaagt cgcgctcgga
     4201 gagcgtgtcg aggaggcgcg tgtcgacgca gacgaaccgc gggtggtgaa acgtaccggc
     4261 gaggttcttg ccggcggcga ggttcaccgc cgtcttccct cccagcgcgc tgtcggccgc
     4321 ggcgaggagg gtcgtcggga cctggacgac ggggacgccg cgcagcgcga gcgaagccgc
     4381 gaggcccgtc gcgtcgccga ccgtcccccc gccgaacgcc acgatcccgc cgtcgcgcgc
     4441 gaggccccgc gccgccatcc cggaaaggag ccggccgagc tccgtccagc tcttcgctct
     4501 ctctccgtcg gggacgagca gcggatcggg gtcgaggttc cgcgcccgcg cgagcgcggc
     4561 ccggaggcgc tcgccctgcg ccgcgaggac gggggcggac gacaccacga gccagcgccc
     4621 cggggcgatc cgggggaggg cgcccgcaag gcgctcgag
//
LOCUS       DAOTEZ010000348         4640 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_13468_length_4640_cov_5.317993, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000348 DAOTEZ010000000
VERSION     DAOTEZ010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13468_length_4640_cov_5.317993"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..574)
                     /locus_tag="P5164_15495"
     CDS             complement(119..574)
                     /locus_tag="P5164_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45341.1"
                     /translation="MRPLRLHPRLAKRSRRGFRGFPAATVAFYGPDDARATKAVVTIV
                     PSKEADPTFQETFSSGEGDLRGDSLVGERIVAFVERHEARSVFVAEEILGCPHEEGVD
                     YPEGERCPACPFWTERDRWAATKERLDAARGELLTRAISEVEAERKAGE"
     gene            complement(584..2290)
                     /locus_tag="P5164_15500"
     CDS             complement(584..2290)
                     /locus_tag="P5164_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45342.1"
                     /translation="MILAALLVFRLAGESVSPVAEAGLPRAVGSLQKPWVVAAWGEAH
                     PGEAPPRLVCAADSRCWLPAGHGRVDLARGLAHSCNTYFLALARATPEPLRAEALARA
                     GFSVRRPLSPEASIGLGPLDALPRIAPAALLRAYRDLLRRPWPSRDDLRLAVLDGMRA
                     AARSGTGAALGARSRLVKTGTVPALDGRPLATSGWALAATPGGESLALALLPDGTGAR
                     AAELLGEELAPGVGRPSERAARAGRLPPTHVRVRLLSPLRPESVRVTNAGAGPVRLRR
                     RGGRETWLGPGASTEAVPGLDAGPGLLRLDVAPHGLARFYEGSLEVFGTKSAPRLVLS
                     TTEREWVDGVLRGELGGRTPELREELAAAALRYLRAGARHGADHLCDTTHCAVFAGRG
                     PAVAWVTPRRAALLAGERPAAADGLLDDDAWARAVSLAARPGPEHFTGHCGGAPLSPR
                     EVWGRGPREAPPCPRHSPADDAAWERLLPEGALASAFGGPVVEMGARRVDGVRRTRVA
                     TRERIDDLLYDDLHRRLAPFLGWDALPSPPDSFERVSGGWRARGRGRGHRVGLCLGAG
                     SR"
     gene            complement(2304..3821)
                     /locus_tag="P5164_15505"
     CDS             complement(2304..3821)
                     /locus_tag="P5164_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45343.1"
                     /translation="MKPTPFLVPLLLSLPLAAAEPLLPLPEAPVVVVSTDPAALDRAL
                     AGGFRRALSGTLPEDDPVARGLRQTRVGGKLDDQRALLAADLPLSWTEIAATKPTALG
                     LALLSAGDLEAVLALRTPLAQLPFALPAGEPRSRNGVPYAFVARGAGDERTLDRRMGL
                     AWARLDGTLIVATSERALHLALDRAAAGKGFGAFLPGLVSARLDLAALRADRYFRREF
                     LFAEGVRGADRGILLCALRAEEGRLVEVREGESGSPPAAVRWSLDGRNVAAAGWEADG
                     QRFPEALRRGLLEPLPAPSERPRLARRPLPDPGAAQGERYLVDLTQAVPPEGEGDAAE
                     LPRWAALFGELKLEGWGWEVGADGARRLLARIPKEHEAAFLALARETLARRAGRADSS
                     PAALTVGPALPALAWSRRGDWLWLAADEAHLTKSPAPAAEAGLVRWSRLDLDAVRAEQ
                     GAWRDAEGASFEGNVRPFSDRILGLLGWAPKSSAVAVERRQEGSRFRERIVFSSR"
     gene            complement(3818..>4640)
                     /locus_tag="P5164_15510"
     CDS             complement(3818..>4640)
                     /locus_tag="P5164_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45344.1"
                     /translation="LLAGVDRPAAIRWFRRLPDPAYFSEVRDRSDLLVAMHDANGARG
                     EWVAARARLALTEEQELAAFDAWRALGGGAASAPAWWTTARTFWERDPAGLASWGGEL
                     ARHLVDHPYDRHAARVVTRSLAPAPARFVAPATLASGGADEAAAGWRVARAELSRDVR
                     AARAALRSTWFDADELRGRDRPDAEVDGLLADLARIGAATGEEGLAERALSALEDRRS
                     PSVPALRTEIEALRLAAAPRPEPFLGGSGRPVVRLLPKDLTWDLYARVLNAEDVP"
BASE COUNT          585 a         1648 c         1935 g          472 t
ORIGIN      
        1 gttcgtcccg gagccgctcc catggaaagg cagcgtagca ccggggcgcg tgccgcggcg
       61 cgtcggcgca ccgatcggcc gctcgattcg atccgtcagg cgcggccggt cgcgacggct
      121 actctccggc cttcctctct gcctcgacct ccgagatcgc gcgcgtgagg agctcgccac
      181 gcgcggcgtc gagccgttcc ttcgtcgcgg cccagcggtc ccgctcggtc cagaagggac
      241 acgcggggca gcgctcccct tcggggtagt cgaccccctc ctcgtgcggg cagccgagga
      301 tctcctcggc gacgaagacg ctgcgggcct cgtgcctttc gacgaaagcg acgatcctct
      361 cgccgacgag cgagtccccc cggaggtctc cttccccgga gctgaaggtc tcctggaacg
      421 tcgggtcggc ttccttcgac gggacgatcg tgacgacggc cttcgtggca cgtgcgtcgt
      481 cgggaccgta gaaggcgacg gtcgccgccg ggaagccgcg gaacccgcgg cgcgagcgtt
      541 tcgcgaggcg agggtggagc cggaggggtc tcacgcgcgg agactagcgc gacccggctc
      601 cgaggcagag ccccactcga tgcccccgcc cacggccgcg ggcgcgccat ccgccggaga
      661 ctcgctcgaa cgagtcgggc ggggagggga gcgcgtccca cccgaggaac ggcgcgaggc
      721 ggcggtggag gtcgtcgtag aggaggtcgt cgatcctctc ccgggtcgcg acccgggtcc
      781 gcctcacgcc gtcgacgcgc cgcgcgccca tctcgacgac ggggcctccg aaggccgatg
      841 cgagcgcccc ctcgggcagg aggcgctccc atgctgcgtc gtccgccgga gagtggcgag
      901 ggcagggggg cgcctcgcgc ggaccgcggc cccagacctc gcgcggagac aggggggcgc
      961 caccgcagtg gccggtgaaa tgctcgggcc ccgggcgcgc ggcgagagag acggcgcgcg
     1021 cccacgcgtc gtcgtcgagg agaccgtccg cagcggcggg acgttcgccg gcgaggagcg
     1081 cggcgcgccg cggcgtgacc cacgcgacgg cggggccccg cccggcgaag acggcgcagt
     1141 gggtcgtgtc gcagaggtgg tccgcgccgt ggcgggctcc ggcacgcagg taccggaggg
     1201 ccgccgccgc gagctcctcg cgaagctccg gcgtgcgtcc ccccagctcg ccgcggagga
     1261 cgccgtcgac ccattcccgc tcggtcgtcg aaaggacgag gcgcggggcg ctcttcgtgc
     1321 cgaagacctc gaggcttccc tcgtagaagc gagcgaggcc gtggggcgcg acgtcgagac
     1381 ggaggaggcc gggcccggcg tcgagccccg gcacggcctc cgtgctcgcg cccgggccga
     1441 gccacgtctc gcgcccgccc ctccggcgaa gccggacggg gccggctccg gcgttcgtca
     1501 cgcggaccga ctccggccgg agcggcgaga ggagacggac gcggacgtgc gtcgggggaa
     1561 ggcgcccggc gcgagcggca cgctcggagg ggcggccgac gcccggcgcg agctcttcgc
     1621 cgaggagctc tgcggcgcgg gcgccggtgc cgtcgggaag gagcgcgagc gcgaggctct
     1681 cgccgcccgg cgtcgcggcg agggcccacc cggaggtggc gagagggcgg ccgtcgagcg
     1741 ccgggacggt ccccgtcttg acgaggcggc tgcgcgcgcc gagcgcggcg cccgtgccgc
     1801 tccgcgccgc ggcgcgcatt ccgtcgagga cggcgagccg gagatcgtcg cgcgagggcc
     1861 aggggcggcg gaggagatcg cgatacgccc ggaggagcgc cgccggggcg atccgcggga
     1921 gcgcgtcgag cgggccgagg ccgatcgagg cctcggggga gaggggccgc cgaacggaga
     1981 acccggcgcg ggcgagggct tccgcgcgga gcggctccgg cgtcgcgcgg gcgagggcga
     2041 ggaagtaggt gttgcacgag tgagctagcc cccgcgccag gtcgacgcgg ccgtgccccg
     2101 cggggagcca gcagcgcgag tcggcggcgc acacgaggcg cggaggggcc tcgccggggt
     2161 gggcctcgcc ccaggcggcg acgacccacg gcttctggag cgagccgacg gcgcggggga
     2221 ggcccgcctc cgcgacgggg gagaccgact cccccgcgag gcggaagacg aggagggcgg
     2281 cgaggatcac cggcaggaaa ggatcaccgc gaggagaaga cgatcctctc gcggaagcgg
     2341 ctcccctcct ggcgtctctc gacggcgacg gccgagctct tcggggccca gccgaggagt
     2401 ccgaggatcc ggtcggagaa ggggcggacg ttcccttcga aggaggcgcc ctcggcgtct
     2461 cgccacgccc cttgctcggc tcggacggcg tcgaggtcga gccggctcca gcggacgagg
     2521 ccggcctcgg cggcgggggc aggagacttc gtcaggtgcg cttcgtctgc ggcgagccag
     2581 agccagtcgc cccggcggct ccaggcgagg gccggaaggg cgggcccgac cgtgagggcc
     2641 gccggcgagg agtcggcgcg gccggcgcgt cgcgcgagcg tctcccgcgc gagggcgagg
     2701 aaggccgcct cgtgctcctt cgggatgcgc gcgaggaggc ggcgcgcacc gtcggcgccg
     2761 acttcccagc cccagccttc gagcttcagc tctccgaaga gcgcggccca gcgggggagc
     2821 tccgccgcgt ccccctcgcc ttcgggcggg actgcctggg tcaggtcgac gaggtagcgc
     2881 tcgccctgcg ccgcgccggg gtcggggagc gggcggcgcg cgagccgggg ccgctcggac
     2941 ggcgcgggga gcggctcgag gagtccgcgg cggagcgcct cggggaatcg ctgtccgtcg
     3001 gcctcccacc cggccgcggc gacgttccgg ccgtcgaggc tccagcgtac ggccgcgggc
     3061 ggggagccgc tctcaccctc gcgcacctcg acgagccgcc cctcttccgc gcgcagggcg
     3121 cagaggagga tgcctcggtc ggcgccgcgg accccttccg cgaagaggaa ctcgcggcgg
     3181 aagtagcggt cggcgcggag ggcggcgagg tcgaggcgcg cggagacgag acccggcagg
     3241 aacgccccga agccctttcc cgcggcggcg cggtcgaggg cgaggtggag agcgcgctcg
     3301 ctcgtcgcga cgatcagcgt gccgtcgagg cgcgcccagg cgaggcccat ccgcctgtcg
     3361 agggtccgct cgtcgcccgc gccgcgggcg acgaacgcgt acggcacgcc gttccggctc
     3421 cgcggctcgc cggcggggag cgcgaagggg agctgggcga gcggcgtccg gagggcgagg
     3481 accgcctcga ggtcgcccgc cgagaggagc gcgaggccga gggccgtcgg cttcgtcgcg
     3541 gcgatctcgg tccacgagag ggggaggtcg gccgcgagga gcgcgcgctg gtcgtcgagc
     3601 ttgccgccga cgcgcgtctg ccgaaggcct ctcgccaccg gatcgtcctc cgggagcgtc
     3661 cccgagaggg cgcgtcggaa ccctccggcg agggcgcggt cgagggcggc cgggtcggtg
     3721 gagacgacga cgacgggcgc ctccgggagc ggaaggagcg gctcggccgc ggcgagcggg
     3781 agcgagagga ggagggggac gaggaacggg gtcggcttca cgggacgtcc tccgcgttca
     3841 ggacccgggc gtagaggtcc caggtcaggt ccttgggaag gaggcggacg accgggcggc
     3901 cgctccctcc gaggaagggc tccggcctcg gcgcggcggc gagccgaagc gcttcgatct
     3961 cggtccgaag ggcgggaacc gagggggatc gccggtcctc cagggcggag agggcgcgct
     4021 cggcgagccc ctcctcgccc gtggcggccc cgatccgggc gaggtcggcg aggagcccgt
     4081 cgacctccgc gtcgggacga tcccggccgc gaagctcgtc ggcgtcgaac caggtggagc
     4141 ggagcgccgc gcgggccgcg cggacgtcgc gggagagctc ggcgcgcgcc acgcgccagc
     4201 cggcggcggc ctcgtcggcc ccgccggagg cgagcgtcgc cggggcgacg aatcgggccg
     4261 gggcgggcgc gaggctgcgg gtgacgacgc gcgcggcgtg gcggtcgtac gggtggtcga
     4321 cgaggtgtcg cgcgagctcg cctccccacg aagcgaggcc ggcggggtcc ctctcccaga
     4381 aggtgcgcgc cgtcgtccac cacgccggag cggaggcggc gccgccgccg agcgcgcgcc
     4441 acgcgtcgaa ggcggccagc tcctgctcct cggtgagcgc gagccgggcg cgcgcggcga
     4501 cccactcgcc gcgcgcgccg ttcgcgtcgt gcatcgcgac gaggaggtcg gagcgatcgc
     4561 ggacctcgga gaagtacgcc gggtcgggga gacggcggaa ccagcggatc gccgccgggc
     4621 ggtcgacccc cgcgaggagg
//
LOCUS       DAOTEZ010000349         4630 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_13515_length_4630_cov_5.006339, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000349 DAOTEZ010000000
VERSION     DAOTEZ010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13515_length_4630_cov_5.006339"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1963
                     /locus_tag="P5164_15515"
     CDS             <1..1963
                     /locus_tag="P5164_15515"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY45345.1"
                     /translation="QVTPLRRSLRGRFALIFSALMGALLLVVSGTLILVRVRDHRDLL
                     ETYATGFAKATNNQLCNAWRLYYRSGAYKFRDIVRNTMTLNEDLHRLFILSVSGEVLF
                     DSMESPDLMLLPELPRRRLADPELVTVAGLQDDWIDRRDLAGVGRVMMVSTPYFEEWG
                     RHPYSVLYIFKYDKIWQRVTSDLRSTLLLVLLALATVAVFASWMAGRVARPLLTLTAG
                     VRSFSEGKPGSLPQVRTGDEIETLAEAFNRMAERIQQQVERLETANRELATLDRMKTD
                     LLANVSHELRTPLAAIRGYVEFIQEGQLGPTTDAQRKGLEVCLRNSERLTKTINMLLD
                     FSRMELGRVPIRTAPFQIGRLVAQIVAGIESEARKRSIRLVAVVDRSLKPVDGDRDRL
                     TQVLENLITNALKFTPDGGEVEVGARPARTAGRVEVWVSDTGIGIPPEERARVFEKFY
                     QTDGSATRKFGGIGLGLAIVKSILDAHGADIVVDGRPGGGTVFRFSLPVAETRTESGV
                     FHGLLPAEGDAAPVLAIDDDPDFLLILKETLEKHGFTVRTATTAEEGLAAAREQAPLL
                     VVLDIRLPDRDGLDLLHELREDAATRQTPILIASVVDERLEGLRLGAVEYLVKPIDRG
                     RLVEAAVRALHSRGKRLPSGEIPAAIEPA"
     gene            2039..2422
                     /locus_tag="P5164_15520"
     CDS             2039..2422
                     /locus_tag="P5164_15520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY45346.1"
                     /translation="MSQQKRTILIVDDEEDVLDLLQLVFETSGFLVRRASTGKSAVSS
                     AWEQPPDVVLLDVMMPEMDGWQVLRTLKGDERTRNVPVVMLSARAERRDKMIGLQEGA
                     EGYIAKPFSPAEVVREVQSFLERGS"
     gene            2419..3747
                     /locus_tag="P5164_15525"
     CDS             2419..3747
                     /locus_tag="P5164_15525"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRY45347.1"
                     /translation="MSSREPLPVASFDDAASMRAEWLQNRARLHDVVTGLPTLPAVVD
                     QVRRHLEDHGSIALLAFSLAMERQVEETWGWQLYDDLIRDFVAGLRSRAAEGGFPGGI
                     FCVPGVRSDEVLCFVPTRPRNGADQAPHEWLGALASRMDAFVSSFLAERLTSVDSYSS
                     HVGQALILFDPKVRVERAVYRGLNEARGEVFRQTASAEERGVEILKGILADRRVVSLF
                     QPIHDLVEEKVTGVEALSRGPAGSGLEDAERLFSLAEKAGLVIPLERLCRRRSLEEAA
                     RSGWDRLVFLNMSPAASQDSDFLEGGLVREVLELHLDPRQVVIEVTERTYAENQELFT
                     RVLGELRREGFHVAVDDLGSGYSNLSALADIHPEFLKFDHLFTKDIHRNRIKQDLLGA
                     ILSFAMKMETQVIAEGIESLDELQALRRLGVPLGQGFYLGRPGPMPTRAA"
     gene            complement(3769..>4630)
                     /locus_tag="P5164_15530"
     CDS             complement(3769..>4630)
                     /locus_tag="P5164_15530"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY45348.1"
                     /translation="EDLLSGRSDVVQSNAKATEEALRDRSGYEVRRHGSVSVKFLFLA
                     LNREAPKDVDGGKNPFRDVRVRRALSLAIDRAALARRLSAPAIPMGQLVPPGIFGHDP
                     SAKALRHDPVEARRLLAEAGWPDGFALELAARGLFAEAAGIVAEMLGEVGIRVRVRAL
                     PEGAWFRAMHEGLLTLTISRYGCPTGDASDLFENALRTWDPERRTGLNNYARYSSPEV
                     DRLTDLAGRTLEMSRRQEALRGATARVLEDLPVIPLYVDQDLYAFREGVSWTPRNDNF
                     LIASEIARAR"
BASE COUNT          673 a         1687 c         1577 g          693 t
ORIGIN      
        1 tcaggtgaca ccgctccgcc gctcgctccg gggccggttc gccctgatct tctcggccct
       61 gatgggggct ctcctgctcg tcgtctcggg gacgctcatc ctcgttcggg tccgggacca
      121 cagggacctc ctggagacgt acgcgaccgg gttcgcgaag gcgacgaaca accagctctg
      181 caacgcctgg cgcctctact accgctccgg ggcctacaag ttccgcgaca tcgtgcggaa
      241 cacgatgact ctcaacgagg acctccatcg cctcttcatc ctttccgtct cgggcgaggt
      301 cctcttcgac tcgatggaga gcccggacct catgctcctc ccggagctcc cccggcggcg
      361 cctcgccgac ccggagctcg tgacggtcgc gggcctccag gacgactgga tcgaccgccg
      421 cgacctcgcc ggcgtcggcc gggtgatgat ggtctcgacc ccctacttcg aggaatgggg
      481 acgccacccg tactcggtgc tctacatctt caagtacgac aagatctggc agcgggtcac
      541 ctcggacctc cgctccacgc ttctcctcgt cctcctcgcg ctcgccaccg tcgccgtctt
      601 cgcgtcctgg atggccggcc gcgtcgcacg gcccctgctc acgctgacgg ccggagtccg
      661 ctccttctcg gaggggaagc cggggtcgct cccgcaggtg aggacggggg acgagatcga
      721 gaccctggcc gaggctttca accggatggc ggagcggatc cagcagcagg tcgagaggct
      781 cgagaccgcg aaccgcgagc tcgcgacgct cgaccggatg aagaccgacc tcctcgcgaa
      841 cgtctcccac gagctgcgga cccccctcgc cgccatccgc ggctacgtcg agttcatcca
      901 ggaggggcag ctcgggccga cgacggacgc gcagcgcaag ggcctcgagg tctgcctccg
      961 caactccgag cggttgacga agacgatcaa catgctcctc gacttctccc ggatggagct
     1021 cggccgggtc ccgatccgga cggccccgtt ccagatcggc cgcctcgtcg cccagatcgt
     1081 cgccgggatc gagtcggagg cgcggaagag gagcatccgc ctcgtcgccg tcgtcgaccg
     1141 gagcctcaag ccggtggacg gggaccgcga ccggctcacg caggtcctcg agaacctgat
     1201 cacgaacgcg ctcaagttca cgcccgacgg cggcgaggtc gaggtgggcg cccggcccgc
     1261 gcggaccgcc ggccgcgtcg aggtctgggt ctccgacacc ggaatcggga tcccgcccga
     1321 ggagagggcg cgcgtcttcg agaagttcta ccagacggac ggctccgcca cgcgcaagtt
     1381 cggcggcatc ggcctcgggc tggcgatcgt aaagtcgatc ctcgacgccc acggcgccga
     1441 catcgtcgtc gacgggaggc cgggcggagg gaccgtcttt cgattctcgc tccccgtcgc
     1501 cgagacgcgc accgagtcgg gggtcttcca cgggctcctg cccgccgagg gcgacgcggc
     1561 gcccgtcctc gcgatcgacg acgacccgga cttcctcctc attctcaagg aaacgctcga
     1621 gaagcacggc ttcaccgtcc ggacggccac gaccgccgag gaagggctgg ccgcggcgcg
     1681 agagcaggct ccgctcctcg tcgtcctcga catccgtctg cccgatcgcg acggcctcga
     1741 cctgctgcac gagcttcgcg aggacgccgc gacgcgccag accccgatcc tcatcgcgtc
     1801 cgtcgtcgac gaacggctcg agggcctccg gctcggcgcc gtcgagtacc tcgtcaagcc
     1861 gatcgaccgc ggcaggctcg tcgaagcagc cgtgcgcgcg ctccactcgc gcggcaagcg
     1921 ccttccgtcg ggagagattc cggcggcgat cgagcccgcc tgagcgcccc gcgagggctt
     1981 cgattgctct aagatcgagg cgttcgccag agcttcggac gcggcgagga gggatccgat
     2041 gtcccagcag aaacggacaa tcctgatcgt cgacgacgag gaggacgtcc tcgacctgct
     2101 gcagctcgtc ttcgagacga gcgggttcct cgtccggcgc gcgtcgacgg gcaagtccgc
     2161 cgtgtcgagc gcctgggagc agccgccgga cgtcgtcctg ctcgacgtca tgatgcccga
     2221 gatggacggc tggcaggtgc tgcgcacgct caagggcgac gagcggaccc ggaacgtccc
     2281 ggtcgtgatg ctctccgctc gcgccgagcg gcgcgacaag atgatcggcc tccaggaggg
     2341 cgccgagggc tacatcgcga agcccttctc cccggccgag gtcgttcggg aggtccagag
     2401 cttcctcgag cggggatcgt gagctcgcgc gagccgctcc cggtcgcgtc gttcgacgac
     2461 gccgcctcga tgcgggcgga atggctccag aaccgggccc ggctgcacga cgtcgtcacg
     2521 ggtcttccga cgctccctgc ggtcgtcgac caggtgcgcc gccacctcga ggaccacggc
     2581 tcgatcgccc tgctcgcgtt ctcgctcgcc atggagaggc aggtcgagga gacgtggggc
     2641 tggcagctct acgacgacct catccgggac ttcgtcgccg ggctgcggag ccgggccgcc
     2701 gaaggcgggt ttccgggcgg gatcttctgc gtcccgggag tgcggagcga cgaggtcctc
     2761 tgcttcgtcc ccacgcgtcc gaggaacggc gccgatcagg cgccccacga gtggctcggg
     2821 gcgctcgcgt cgcgcatgga cgccttcgtc tcctcgttcc tcgccgagcg gctgacctcc
     2881 gtcgattcct actcctccca cgtcgggcag gcgctgatcc tctttgaccc gaaggtccgg
     2941 gtcgagcgcg cggtctaccg gggcctgaac gaggcgcggg gcgaggtctt ccggcagacg
     3001 gccagcgccg aggagcgcgg ggtcgagatc ctgaagggga tcctcgccga ccggcgggtc
     3061 gtctcgctct tccagcccat ccacgacctc gtcgaggaga aggtcaccgg cgtggaggcc
     3121 ctctcgaggg ggcccgccgg cagcgggctc gaggacgccg agcggctctt ctcgctcgcc
     3181 gagaaggccg gcctcgtcat ccctctcgag cggctctgcc ggagacggtc gctcgaggaa
     3241 gcggcccgat cgggctggga caggctcgtc ttcctcaaca tgtcccccgc ggcgtcgcag
     3301 gactccgact tcctcgaagg ggggctggtc cgcgaggtcc tcgagctcca cctcgacccg
     3361 aggcaggtcg tcatcgaggt gaccgagcgg acctacgcgg agaaccagga gctcttcacg
     3421 cgagtgctgg gcgagctccg cagggagggt ttccacgtcg ctgtcgacga cctcggctcg
     3481 ggctactcga acctgtccgc gctcgcggac atccaccccg agttcctgaa gttcgaccac
     3541 ctcttcacga aggacatcca ccggaaccgg atcaagcagg acctgctcgg cgccatcctc
     3601 tcgttcgcga tgaagatgga gacccaggtc atcgcggagg ggatcgagtc gctcgacgag
     3661 ctccaggccc tccgcaggct cggcgtcccg ctcggacagg gcttctacct gggccggccc
     3721 ggcccgatgc cgacccgggc cgcctgaccc cccgcgccgg gcgccggctc agcgcgcccg
     3781 cgcgatctcg gaggcgatca ggaagttgtc gtttcgcggc gtccaggaga ccccctcccg
     3841 gaaggcgtag aggtcctggt cgacgtacag cggaatcacg ggcaggtcct cgagcacccg
     3901 cgccgtcgcc ccccgcagcg cctcctgccg cctgctcatc tcgagcgtcc ggccggccag
     3961 gtcggtgagc cggtccacct ccgggctcga gtagcgcgcg tagttgttca gcccggtcct
     4021 ccgctccggg tcccacgtcc ggagggcgtt ctcgaagagg tcggaggcgt cgcccgtcgg
     4081 acagccgtaa cggctgatcg tgagagtgag gagcccctcg tgcatcgccc ggaaccaggc
     4141 tccctccggg agagcgcgca cgcggacgcg aatcccgacc tccccgagca tctccgccac
     4201 gatcccggcc gcctcggcga agagtccccg ggccgcgagc tcgagcgcga agccgtccgg
     4261 ccagccggcc tcggcgagca gccggcgggc ctcgaccggg tcgtgtcgca gcgccttcgc
     4321 cgacgggtcg tggccgaaga tcccgggcgg cacgagctgg cccatcggga tcgcgggggc
     4381 cgagaggcgg cgggcgagcg ccgcgcggtc gatcgcgagg gagagcgccc gacggacgcg
     4441 aacgtcgcgg aacgggttct tccctccgtc gacgtccttc ggggcctccc ggttcagcgc
     4501 gaggaagagg aacttgacgg agacgctgcc gtgccgccgg acctcgtagc cgctccggtc
     4561 gcgcagcgcc tcttccgtcg ccttcgcgtt cgactgcacg acgtcgctcc gaccggagag
     4621 gaggtcctcg
//
LOCUS       DAOTEZ010000350         4629 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_13519_length_4629_cov_5.862921, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000350 DAOTEZ010000000
VERSION     DAOTEZ010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13519_length_4629_cov_5.862921"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="P5164_15535"
     CDS             <1..561
                     /locus_tag="P5164_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRY45349.1"
                     /translation="SLDMDASDKAARFVRTCNIFNIPIVTLVDVPGFLPGVAQEHGGI
                     IRHGAKMLFAYSAATVPKMTVILRKAYGGAYLAMCSKDLGADSVLAWPTAEIAVMGPE
                     GAARVLHRRELEAAKSAKEKNALLAEKAAEYRKLHANPYRAAAALYVDDVIRPSWTRR
                     LLIERLVTLRAKRDQRPQKKHGNIPM"
     gene            571..894
                     /locus_tag="P5164_15540"
     CDS             571..894
                     /locus_tag="P5164_15540"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HRY45350.1"
                     /translation="MSVYVVALICMSVVMCVMALLVGVLYGFQAITRRQAVAAAKATS
                     AAAASEEGVTPELVAVLAAAATAALGRSVVVRHIRLIRPAGQETWSRVGRIDLLRSHR
                     VDPKR"
     gene            897..1313
                     /locus_tag="P5164_15545"
     CDS             897..1313
                     /locus_tag="P5164_15545"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HRY45351.1"
                     /translation="MKKLRITVSGKVYDVEVQVLQDDEAIGSAASLPYPGAAALPMTE
                     AARPASSPTPSSTPAPGGAGDPNVIASPIAGTVQKVFVTKGMHVEGKTPVVLLDAMKM
                     DTYIYAPRSGTIAEVCVEAGASVQVRDPLIRYAPEG"
     gene            1372..2433
                     /locus_tag="P5164_15550"
     CDS             1372..2433
                     /locus_tag="P5164_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HRY45352.1"
                     /translation="MIAVAFLLLWLAIKKEFEPLLLVPIGFGALMTNLPLNGLMNLPA
                     EGAPGGLFHYLFQGVELEIFPPLIFLGLGALTDFGPLLANPKTFLLGGAAQLGVFGTF
                     LAAGWLGFTPKEAASIGIIGGADGPTSIYLTIKLAPHLLGAIAVSAYSYMALVPLIQP
                     PIMRALTTKKERLIRMEANFDVPKWLRISFPIVCTVICALIVPAATSLIGLLMFGNLL
                     RECGVTERLTRSAQNELINLVTIVLGLSVGVTMDGDSFLRAATLKILVLGCVAFAVST
                     AGGVLLGKLMSRLPGTPVNPLIGAAGVSAVPMAARVAHNVGQAEDPDNYLLMHAMGPN
                     VAGVIGTAVAAGSLLALLR"
     gene            2521..3903
                     /locus_tag="P5164_15555"
     CDS             2521..3903
                     /locus_tag="P5164_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017178392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HRY45353.1"
                     /translation="MTVPWYGILPFAALLAAIATFPLMHATKHHWEKRRFQLLVALVL
                     GLPMAAWCWSLGGRTEVIHALVEYASFIALLFSLFVVSGGVFVAGDIQATPRNNTLFL
                     GAGAVLASLIGTTGAAMLLVRPLLNTNRERKHKVHTVVFTVLVVANCGGLLTPLGDPP
                     LFLGLLRGVPFLWTLKLFPEWLFVNGMLLLTYYALDSAAFRKEEMPDLVKDAADVKPL
                     ALHGKGSIALLGVIVAAVALAPSVDLHAIREGHASLAAWIPFREIILLAAAALSFFLG
                     DRKARFEDNRFEWGPIQEVAALFIGIFLTMVPALAYLAQKAPELPLNRVTFFVFAGSL
                     SSVLDNAPTYVTFFELAKALGGEPAVAGVREVFLVSISLGAVFCGAMTYIGNGPNFMV
                     KAIAESDGVAMPSFGGYVARWAFVYLLPILALMVGVFIVETTWVRVTSAVLATFLAGG
                     RVRSILALRR"
     gene            3933..4316
                     /gene="crcB"
                     /locus_tag="P5164_15560"
     CDS             3933..4316
                     /gene="crcB"
                     /locus_tag="P5164_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HRY45354.1"
                     /translation="MKTLALVFLGGGIGASLRHLLGGAAQALSRSATFPWGTFAVNVT
                     GCFAIGLLAGLAEERGVPSGNARSFLFVGVLGGYTTFSSFGNETINLLRGGEALSAAA
                     NAGGQLVLGLLAVALGRASARLLWR"
     gene            4319..>4629
                     /locus_tag="P5164_15565"
     CDS             4319..>4629
                     /locus_tag="P5164_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HRY45355.1"
                     /translation="MTLPEDGALLRVFVGESDRHEGKLLYEWLVLKAREKGLAGATVL
                     RGVMGFGAHSRLHTFKIERLSEDLPVVVEIVDAREKLEAFLEEIEGHVPEGLATMEKA
                     FV"
BASE COUNT          605 a         1739 c         1513 g          772 t
ORIGIN      
        1 tcgctcgaca tggacgcgtc cgacaaggcg gcccgtttcg tccgcacgtg caacatcttc
       61 aacatcccga tcgtgactct cgtcgacgtc cccgggttcc tcccgggcgt cgcgcaggag
      121 cacggcggga tcatccggca cggcgcgaag atgctcttcg cctactccgc cgccaccgtc
      181 ccgaagatga ccgtcatcct ccggaaggcc tacggcggcg cctacctcgc gatgtgctcg
      241 aaggacctcg gggccgactc ggtcctcgct tggccgacgg ccgagatcgc cgtcatgggg
      301 ccggaggggg ccgcccgcgt cctccaccgc cgcgagctcg aggcggcgaa gtcggcgaag
      361 gagaagaacg ccctcctcgc cgagaaggcc gcggagtacc ggaagctcca tgcgaacccg
      421 taccgggccg ccgcggccct ctacgtcgac gacgtcatcc ggccgtcctg gacgcggcgc
      481 ctcctcatcg agcgcctcgt caccctgcgg gccaagcgcg accagcgacc gcagaagaag
      541 cacggcaaca tcccgatgta gggcgcggag atgtcggtct acgtcgtcgc cctgatctgc
      601 atgagcgtcg tgatgtgcgt catggcgctc ctcgtcggcg tcctctacgg cttccaggcc
      661 atcacccgga ggcaggccgt cgcggccgcg aaggcgacct cagcggcggc cgcgtccgag
      721 gagggcgtca cgcccgagct cgttgcggtc ctcgccgcgg cggcgaccgc cgctctcggc
      781 cggtccgtcg tcgtccgcca catccgcctc atccgccccg cgggccagga gacctggtcc
      841 cgcgtcgggc ggatcgacct cctgcggtcc caccgggtgg acccgaagag atagcgatga
      901 agaagcttcg tattaccgtc agcggcaagg tctacgacgt cgaggtccag gtccttcagg
      961 acgacgaggc gatcggctcg gccgccagcc tcccgtaccc cggggccgcc gctctcccga
     1021 tgacggaggc cgcccgtccg gcgtcctcgc cgactccgtc gtccacgccg gcgcccggcg
     1081 gcgccggcga cccgaacgtc atcgcctcgc cgatcgccgg caccgtccag aaagtcttcg
     1141 tgacgaaggg gatgcacgtc gaggggaaga cccccgtcgt cctcctcgac gccatgaaga
     1201 tggacaccta catctacgcc ccgcgcagcg ggacgatcgc ggaggtctgc gtggaggcgg
     1261 gcgcgtccgt ccaggtgcgc gacccgctca tccgctacgc cccggagggg tgacgtggag
     1321 aagctgctcg cgcagaccgg cttcctcgcg ctgacgtggc gcgagggggt gatgatcgcg
     1381 gtcgcgttcc tcctcctctg gctcgccatc aagaaggagt tcgagccgct cctcctcgtc
     1441 ccgatcgggt tcggggcgct catgacgaac ctccccctga acggcctgat gaacctcccg
     1501 gccgaaggcg cgccgggcgg gctcttccac taccttttcc agggggtcga gctcgagatc
     1561 ttcccgccgc tcatcttcct cggcctcggg gcgctgaccg acttcgggcc gctcctcgcg
     1621 aacccgaaga cgttcctcct cggcggcgcc gcccagctcg gcgtcttcgg gacgttcctg
     1681 gcggccggct ggctcggctt cacgccgaag gaggcggcct cgatcgggat catcggcggc
     1741 gcggacgggc cgacgtcgat ctacctgacg atcaagctcg ccccccacct cctcggggcg
     1801 atcgccgtat ccgcctactc ctacatggcg ctcgtgccgc tcatccagcc gccgatcatg
     1861 agggccctca cgacgaagaa ggagcgcctc atccggatgg aggcgaactt cgacgtcccg
     1921 aagtggctcc ggatctcgtt cccgatcgtc tgcacggtca tctgcgcgct gatcgtcccg
     1981 gcggccacct cgctcatcgg cctcctgatg ttcgggaacc tcctccgcga atgcggcgtc
     2041 acggaacgcc tgacccgctc ggcccagaac gagctgatca acctcgtcac gatcgtcctc
     2101 ggcctctcgg tcggcgtcac gatggacggc gactcgttcc tccgggcggc gaccctcaag
     2161 atcctcgtcc tcggctgcgt cgccttcgcc gtctcgacgg cgggcggcgt cctcctcggg
     2221 aagctgatgt cccgcctgcc ggggacgccg gtcaacccgc tcatcggcgc cgcgggcgtc
     2281 tcggccgtcc cgatggcggc gcgcgtcgcc cacaacgtcg ggcaggccga ggaccccgac
     2341 aactacctcc tgatgcacgc gatggggccg aacgtcgccg gggtcatcgg caccgcggtc
     2401 gcggccggga gtctcctcgc gctcctcagg tagcctcttc cgccgcgccg gctcccgggc
     2461 cggcgcggcg gtctcccttc cgattccccc gcccgcaccc ggtgcgggcc cgaggtcgag
     2521 atgacggtcc cctggtacgg aatccttccg ttcgcggcgc tcctcgcggc gatcgcgacg
     2581 ttccccctga tgcacgcgac gaagcaccac tgggagaagc ggcgcttcca gctcctcgtc
     2641 gccctcgtcc tcggtctccc gatggcggcc tggtgctggt cgctcggcgg ccgcaccgag
     2701 gtgatccacg ccctcgtcga gtacgcctcg ttcatcgcgc tcctcttctc cctgttcgtc
     2761 gtgagcggcg gcgtcttcgt ggcgggcgac atccaggcga ccccgcggaa caacaccctc
     2821 ttcctgggcg ccggggccgt cctcgcctcg ctcatcggga cgaccggggc cgcgatgctc
     2881 ctcgtgaggc cgctcctgaa cacgaaccgg gagcggaagc acaaggtcca tacggtcgtc
     2941 ttcacggtcc tcgtcgtcgc gaactgcggc gggctcctga cgccgctcgg cgaccctccg
     3001 ctcttcctcg gcctcctgcg cggcgtgccg ttcctctgga cgctgaagct cttccccgag
     3061 tggctcttcg tgaacgggat gctcctcctg acctactacg ccctcgattc cgccgcgttc
     3121 cggaaggagg agatgcccga cctcgtgaag gacgccgccg acgtcaagcc gctcgccctc
     3181 cacgggaagg ggagcatcgc gctcctcggc gtcatcgtcg ccgccgtcgc gctcgccccg
     3241 tccgtcgacc tgcacgcgat ccgcgagggg cacgcgtccc tcgccgcgtg gatcccgttc
     3301 cgggagatca tcctcctggc ggcggcggcc ctctccttct tcctcggcga ccggaaggcc
     3361 cggttcgagg acaaccgatt cgagtggggg ccgatccagg aggtcgccgc cctcttcatc
     3421 ggcatcttcc tgacgatggt cccggctctc gcctatctcg cccagaaggc gccggagctg
     3481 ccgctgaacc gagtgacgtt cttcgtcttc gccggtagcc tctcctcggt cctcgacaac
     3541 gctccgacct acgtgacgtt cttcgagctc gcgaaggcgc tcggcgggga gccggccgtc
     3601 gcgggcgtga gggaggtctt cctcgtctcg atcagcctcg gggcggtctt ctgcggcgcg
     3661 atgacctaca tcgggaacgg cccgaacttc atggtcaagg cgatcgcgga gtcggacggc
     3721 gtcgcgatgc cgagcttcgg cggctacgtc gcccggtggg ccttcgtcta cctcctgccg
     3781 atcctggcgc tgatggtcgg ggtcttcatc gtggagacga cctgggtgcg cgtgacgagc
     3841 gccgtcctcg cgaccttcct cgccggtgga cgtgtccgga gcatcctggc cctccgccgc
     3901 tgaccagcgc cgatgctagg ctcccccgac cgatgaagac cctcgccctc gtcttcctcg
     3961 gaggcggcat cggcgcctcg cttcgccacc tcctcggcgg cgccgcccag gcgctttcgc
     4021 ggagcgcgac gtttccctgg gggacgttcg ccgtgaacgt caccggctgc ttcgcgatcg
     4081 gcctcctcgc cgggctggcg gaggagcgcg gcgtcccgtc cgggaacgcc cgctccttcc
     4141 tcttcgtcgg ggtcctcggc gggtacacga ccttctcctc cttcggcaac gagactatca
     4201 acctcctccg cggaggcgag gccctctccg ccgctgcgaa cgcggggggg cagctcgtcc
     4261 tcggcctcct cgcggtggcg ctcggccggg cctccgcccg gctcctctgg aggtgacggt
     4321 gacccttccc gaagacggcg ccctcctccg ggtcttcgtc ggcgagagcg atcgccacga
     4381 ggggaagctc ctctacgagt ggctcgtcct gaaggcacgg gagaagggcc tggcgggggc
     4441 gacggtcctc cggggcgtga tgggcttcgg ggcgcacagc cgcctccaca cgttcaagat
     4501 cgagcggctg tccgaggacc tccccgtcgt cgtcgagatc gtcgacgcgc gcgagaagct
     4561 ggaggcgttc ctcgaggaga tcgaggggca cgtccccgag gggctcgcga cgatggagaa
     4621 ggcgttcgt
//
LOCUS       DAOTEZ010000351         4616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_13574_length_4616_cov_5.932032, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000351 DAOTEZ010000000
VERSION     DAOTEZ010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13574_length_4616_cov_5.932032"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="P5164_15570"
     CDS             complement(<1..507)
                     /locus_tag="P5164_15570"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRY45356.1"
                     /translation="MKIRFVVLLVAVALACPACRRGAEAPGGVGTFPDAPVVLVSIDT
                     LRSDRLPAYGFTKVRTPHLDRLAADAWLFEKAFAPTPMTLPSHTSMLTGMIPPEHGVR
                     NNSGFTFRGEAHPSLPKLLKEHGRATGAAVSTWVLRRETGIGALFDDYEDSVPTDPGV
                     ATVRYQRPG"
     gene            complement(504..2705)
                     /locus_tag="P5164_15575"
     CDS             complement(504..2705)
                     /locus_tag="P5164_15575"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRY45357.1"
                     /translation="MRRTGLRAAALGLAAASLLAPACRRPAAPDGGFRASPGAPVFLI
                     VVDTLRSDRLPFYGYVSGVTPALSALREDAILYESAWSPVPLTLPSHSSLFTGVPPGV
                     HRVLDNGGYRLGAAPPTLAELLKREGYATGGAVSCVVLSGSTGISRGFDAWEDRIEPS
                     APGLAANRVQRPGQETARHLEAWLGSVKGERVFGFLHLFEPHAPYEAPEPFRSRFADP
                     YDAEVATADAAVGAFLDALKRRGLYEKSLVVFLSDHGEGLGDHGEEEHGVFLYREAIQ
                     VPLLVKLPSAKPGERPPYAGTRVETPVELLDVLPTVADVVGLPGFTPREGSVSLARLA
                     AGAASPGRRLFAETFYPRIRFGWSELRSVVDGTWHYVDAPRPELYDLATDPGETKNRI
                     EEKAGPLRAMKAEVERRKSPFVEPSAVDEEEAKKLASLGYVTMTTAGGGALPDPKDEI
                     GTLEQLKRGLGLLKGGRTAESVEELRKLLERNPRLVDAWEAYAQGLARLGRADEAVAA
                     IRKTVELSPADRTNYLLSVANLCLQVGRPDEAIRHADLAIARGDTGGHEVKARALLGK
                     GDLAGAEAEARRSLETGEGRKRSWLVLADVLASGGRFAEALAMTEKLRETVGERGLDD
                     LAGYRFLRGNLLGRLGRWEEAEAEILEETRRFPGYVPAWQTLVLLQASRGRGAEARKT
                     VDEMIAAAEGPAAYAAAAELLERLGDAASAARLRAGARVGPGAAPGGRRTP"
     gene            complement(2702..3616)
                     /locus_tag="P5164_15580"
     CDS             complement(2702..3616)
                     /locus_tag="P5164_15580"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY45358.1"
                     /translation="MVVETERRRTPLRAPEAVDAEAAKKLQALGYLTGTARDDGGARP
                     DPKEAIGSLTTLQTATRMHEAGRSAEAVPLLQEVLRANPRLVDAWEVLANAYERLGRM
                     DDALAALKQTVALSPPGRSNYVADVAALAFRAGRLDEARSHAELARTLGERRADVVLA
                     RVALREGKLDEALRVADEGLARGGAPTAGLHVARAEVLGRRERLPEAEREYRAELEAH
                     PTSVEALSGLAIIAAASGDLAEAARRAAAIPRTVPTPDGYVTAIVSLRSFGRPGEAAS
                     LLAEAKRAFPGDFRVARLEAGAKAGGTR"
     gene            complement(3893..>4616)
                     /locus_tag="P5164_15585"
     CDS             complement(3893..>4616)
                     /locus_tag="P5164_15585"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRY45359.1"
                     /translation="ETPALDAFRKEAVLFERAYTHVPLTLPAHASLFTGLEPGGHGVL
                     DNAGYRLPAGAPTLAELLKGRGYATGGAVSAAVLSGASGIGRGFDLWQDRVEARGEVS
                     MLDFVERPGGETAALLTAWIREQAGRPFFAFLHTYEPHAPYAAPEPHRSRWSDPYDAE
                     VAASDEIVGSFFQELKRLGVWDRAIVVFLSDHGEGLGDHGEQQHGIFLYRESIQVPLL
                     MKLPGNALAGGTVAAPVQLTDL"
BASE COUNT          567 a         1711 c         1747 g          591 t
ORIGIN      
        1 gcccggcctc tggtagcgca ccgtggcgac gccggggtcc gtcgggacgg agtcctcgta
       61 gtcgtcgaag agcgcgccga tccccgtctc cctccggagg acccacgtcg agacggccgc
      121 tccggtcgcc cggccgtgct ccttcaggag cttcgggagg ctcgggtggg cctcgccgcg
      181 gaacgtgaag ccggaattgt tccgcacgcc gtgctcggga gggatcatcc ccgtgagcat
      241 cgacgtgtgc gacggaaggg tcatcggcgt cggcgcgaag gccttctcga agagccaggc
      301 gtcggcggcg agcctgtcga ggtgcggcgt tctcaccttc gtgaagccgt aggccgggag
      361 ccggtcggag cggagcgtgt cgatggagac gaggacgacc ggggcgtccg ggaacgtccc
      421 gacgccgccg ggcgcctcgg cgccgcgacg gcaggccggg cacgccaggg cgacggcgac
      481 gaggagaacg acgaagcgaa ttttcacggt gtcctccggc cgcccggcgc ggcgccgggc
      541 cccacgcgcg ccccggcccg cagccgcgcg gcgctcgcgg cgtcgccgag ccgctccagc
      601 agctccgcgg cggcggcgta cgccgccggt ccttccgccg cggcgatcat ctcgtccacc
      661 gtcttccgcg cctcggcgcc ccgcccgcgg ctcgcctgca ggaggacgag cgtctgccac
      721 gccgggacgt agcccgggaa gcggcgcgtc tcctcgagga tctccgcctc ggcctcctcc
      781 catcggccga gccggccgag gaggttcccg cgcaggaagc ggtagccggc gaggtcgtcg
      841 agaccgcgct cgccgaccgt ctcccggagc ttctccgtca tcgcgagcgc ctccgcgaag
      901 cggccgccgc tcgccaggac gtcggcgagg acgagccagg agcgcttgcg cccctcgccg
      961 gtctcgaggg agcgcctcgc ctccgcctcg gcgccggcga ggtctccctt cccgaggagc
     1021 gcccgcgcct tcacctcgtg cccgcccgtg tcgccgcggg cgatcgcgag gtcggcgtgg
     1081 cggatcgcct cgtcggggcg cccgacctgg aggcagaggt tcgcgaccga aaggaggtag
     1141 ttcgtccggt cggccggcga gagctccacc gtcttccgga tcgcggcgac cgcctcgtcg
     1201 gcccgcccca ggcgcgcgag cccctgcgcg tacgcctccc aggcgtcgac gaggcgcggg
     1261 ttcctctcga ggagcttccg gagctcctcg acgctttcgg cggtccgtcc ccccttcagg
     1321 aggccgaggc cgcgcttgag ctgctcgaga gtcccgatct cgtccttcgg gtctgggagc
     1381 gctccgccgc cggccgtcgt catcgtcacg tagccgagcg aagcgagctt cttcgcctcc
     1441 tcttcgtcca ccgccgaggg ctcgacgaac gggctcttcc gccgctcgac ctcggccttc
     1501 atcgcccgga gcggcccggc cttctcctcg atcctgttct tcgtctcgcc cgggtcggtc
     1561 gcgaggtcgt agagctcggg gcgcggggcg tcgacgtaat gccacgtccc gtccaccacc
     1621 gagcgcagct cgctccagcc gaagcggatc cgcgggtaga acgtctcggc gaagaggcgc
     1681 cgcccgggcg aggccgcgcc cgcggcgagg cgggccagcg agacgctccc ctcgcgcggg
     1741 gtgaagcccg ggaggccaac gacgtcggcc accgtcggga ggacgtcgag gagctcgacg
     1801 ggcgtctcca cgcgcgtccc ggcgtacggg ggccgctcgc cgggcttcgc cgacgggagc
     1861 ttcacgagga gcggcacctg gatcgcttcg cggtagagga agacgccgtg ctcctcctcg
     1921 ccgtggtcgc cgagcccctc tccgtggtcg gagaggaaga cgacgagcga cttctcgtag
     1981 agcccgcggc gcttcagcgc gtcgaggaac gcgccgaccg ccgcgtcggc cgtcgccacc
     2041 tccgcgtcgt acgggtcggc gaagcggctc cggaacggct ccggcgcctc gtacggcgcg
     2101 tgcggctcga agaggtggag gaacccgaag acgcgctccc ccttcacgct cccgagccac
     2161 gcctcgaggt gccgcgccgt ctcctgcccg ggccgctgga cgcggttcgc cgcgagcccc
     2221 ggcgccgacg gctcgatccg gtcttcccag gcgtcgaagc cgcgagagat gccggtgctc
     2281 cccgagagga cgacgcacga gaccgccccg cccgtcgcgt agccctctct cttcaggagc
     2341 tcggcgagcg tcggtggcgc ggccccgagg cggtagccgc cgttgtcgag gacgcggtgc
     2401 accccgggcg ggacgccggt gaagagcgag gagtgcgacg ggagcgtgag cgggacgggg
     2461 ctccaggcgc tctcgtagag gatcgcgtct tcgcgcagcg cggagagcgc gggcgtaacg
     2521 cccgagacgt agccgtagaa cgggagtcgg tccgagcgga gggtgtcgac gacgatcagg
     2581 aagacgggcg cgcccggcga ggcgcggaac ccgccgtccg gcgccgccgg gcgacggcac
     2641 gccggagcga ggagcgacgc ggccgcgagg ccgagcgccg cggcgcggag gccggtgcgc
     2701 ctcatcgcgt ccccccggcc ttcgcgcccg cctcgaggcg cgcgacgcgg aaatcgcccg
     2761 ggaaggcgcg cttcgcctcg gcgaggagcg acgcggcctc accgggccgc ccgaagctcc
     2821 gaaggctgac gatcgcggtg acgtagccgt ccggcgtcgg gacggtgcgc gggatcgccg
     2881 cggcgcgccg cgccgcttcg gcgaggtcgc cgctcgcggc cgcgatgatc gcgaggccgg
     2941 agagcgcctc gacgctcgtc ggatgcgctt cgagctccgc ccggtattcc cgctcggcct
     3001 cgggaaggcg ctctcgccgt ccgaggacct cggccctcgc gacgtggagg ccggccgtcg
     3061 gagcgccccc gcgcgcgagc ccctcgtccg cgacccggag cgcctcgtcg agcttccctt
     3121 cgcggagcgc gacgcgcgcg aggacgacgt cggcgcgccg ctcgccgagc gtccgcgcga
     3181 gctccgcgtg gcttcgcgcc tcgtcgagcc gaccggcgcg gaaggcgagc gcggcgacgt
     3241 cggccacgta gttcgaccgg cccgggggcg agagggcgac ggtctgcttc agcgccgcga
     3301 gcgcgtcgtc catccggccg agccgctcgt aagcgttcgc gaggacctcc caggcgtcca
     3361 cgagtcgggg attcgcgcga aggacctcct gcaggagagg caccgcctcc gcgctccgcc
     3421 ccgcctcgtg catgcgggtc gccgtctgga gcgtcgtgag ggagccgatc gcctccttcg
     3481 ggtcgggccg ggcgccaccg tcgtcccgcg ccgtccccgt caggtagccg agggcctgga
     3541 gcttcttcgc cgcctcggcg tcgaccgcct ccggcgcgcg gagcggcgtt cttcgccgct
     3601 cggtctcgac gaccatcgcg cggagcgcgg gcgggcgggt ggatgcgagg ttcgtcttct
     3661 gggccgggtc ggccgcgacg tcgtagagct ccggcgtcgg agcctcgatg tagtgatagc
     3721 ggtccgagac gagggcccgg agctcgctcc agccgagccg gatgcgcggg ctgtagttct
     3781 ccgcgaagac gcgccggtcg ggcgtcgggg cgcctccggc gagcgcggcg agcgagaagg
     3841 tcccgggccg cgccggggcg tccgggacgc ccgcgaggcc gaggagcgtc ggctagaggt
     3901 cggtcagctg gaccggcgcc gcgaccgtcc cgcccgcgag ggcgttcccg gggagcttca
     3961 tcaggagcgg gacctggatc gactcccggt agaggaagat gccgtgctgc tgctccccgt
     4021 ggtcgccgag cccctcgccg tggtcggaga ggaagacgac gatcgcccgg tcccagacgc
     4081 cgagccgttt cagctcctgg aagaacgacc cgacgatctc gtccgaggcg gcgacctccg
     4141 cgtcgtacgg gtcgctccag cgcgagcggt gcggctccgg cgccgcgtac ggagcgtgcg
     4201 gctcgtaggt gtggaggaac gcgaagaagg ggcgtccggc ctgctcccgg atccacgccg
     4261 tcaggagcgc cgccgtctcc cctccgggcc gctcgacgaa gtcgagcatc gagacctcgc
     4321 cgcgcgcctc cacccggtcc tgccagaggt cgaagccgcg cccgatgccg ctcgcaccgg
     4381 aaaggaccgc cgcggagacg gcgccgcccg tcgcgtagcc ccgccccttc aggagctcgg
     4441 caagcgtcgg cgcgccggcc gggagccgat agcccgcgtt gtcgaggacg ccgtgcccgc
     4501 ccggctcgag gccggtgaag agcgaggcgt gcgccgggag ggtgagcggc acgtgcgtgt
     4561 aggcgcgttc gaagaggacg gcctccttcc ggaacgcgtc gagggccggc gtctcg
//
LOCUS       DAOTEZ010000352         4602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_13626_length_4602_cov_6.745986, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000352 DAOTEZ010000000
VERSION     DAOTEZ010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13626_length_4602_cov_6.745986"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="P5164_15590"
     CDS             complement(<1..175)
                     /locus_tag="P5164_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HRY45360.1"
                     /translation="MYTDKSIACIDCGAPFTFTANEQEFYAQKGFSNEPKRCKICRDK
                     RKNSGMGDGGSRQL"
     gene            complement(492..887)
                     /locus_tag="P5164_15595"
     CDS             complement(492..887)
                     /locus_tag="P5164_15595"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HRY45361.1"
                     /translation="MKLTMLKAKIHRATVTRAHLDYEGSVTIDRTLLEAAGILPYERV
                     EIYNVTRGSRLATYAIEGEAGSGAIEINGAAAHLAGPGDLVILAAYCEMTPEEAPHHR
                     PHLVFVDSRNRISETKHAEMPHTLPAGVR"
     gene            complement(890..1735)
                     /gene="panC"
                     /locus_tag="P5164_15600"
     CDS             complement(890..1735)
                     /gene="panC"
                     /locus_tag="P5164_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRY45362.1"
                     /translation="MKTVSTVPDLRSAVASYREKGQRVAFVPTMGALHAGHLALVEQA
                     RRAAGKVVVSIFVNPKQFGPGEDFERYPRDLAADAEKLREAGADLLFAPAVDVMYPPG
                     FSTTVHVAGVSEGMEGARRPGHFDGVATVVARLLGLVLPDVAVFGQKDAQQCAVVMRL
                     VADLGLPVEVLVAPTVRESDGLAMSSRNAYLSPEERKAAPALFRALLATQLVHELGER
                     KAEKLLAAFRLAIGTELRLELDSADLVDAATMRPVEKVDRPVLLAAAVRAGRTRLIDN
                     VVFGG"
     gene            complement(1732..2649)
                     /gene="panB"
                     /locus_tag="P5164_15605"
     CDS             complement(1732..2649)
                     /gene="panB"
                     /locus_tag="P5164_15605"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRY45363.1"
                     /translation="MTRGTTGTATGASSPGTPAPLRLTAPAIRAFKGRRKIAALTAYD
                     APTAALLDAAGVDLLLVGDSIEMVVYGAADTLGASLDRMVAHAKAVSGSAKRALVVGD
                     LPYLTYHTTPEESVRNAARFVVEGGCRAVKLEGGSQRLPMIKAILAAEIPVMGHLGLT
                     PQSVNALGGFKVQGRRATEARRLLDEAKKLADAGVFALVLECVPAELAARITEAIPIP
                     TIGIGAGAGCDGQVLVLHDLVGLAAPGARVPRFVRRYADVGAVIRDAAAAYVADVRSG
                     AFPSEAESFAAEPGEAPPVALYAAGEGRR"
     gene            complement(2810..3469)
                     /locus_tag="P5164_15610"
     CDS             complement(2810..3469)
                     /locus_tag="P5164_15610"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRY45364.1"
                     /translation="MGAPNAEKFPLRHIAIEGPIGVGKTSLAGLLAKRFSGTQFLEDV
                     KNPFLPDFYEGKKGAAFQAQIYFLLSRYGQQQEIAQMDLFKELVVADYTFAKDKIFAN
                     LTLSDEEMRLYEKVWQPLSEALPKPDLVIHLTATVETCLARIKLRGRSFEKSITADYL
                     KQLTEAYNYFFHHYRETPLLVVDTNQIDFVKRKDDLEDLIRQITRPFRGTQFYVPRGS
                     R"
     gene            complement(3482..4333)
                     /locus_tag="P5164_15615"
     CDS             complement(3482..4333)
                     /locus_tag="P5164_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HRY45365.1"
                     /translation="MPSPRDFLSLVVFAHTVFALPFALLSAILAAGGVPDGRTLLWIL
                     LAMVGARSAAMSFNRIVDRHVDAKNPRTSRREIPAGVVSPAAAALFCAASAALFVVAA
                     AQLNRLCLYLSPVALAVVLGYSYAKRFTAAAHLVLGLSLAIAPVGAWVAVTGAFALPP
                     VLLGLAVVFWVAGFDVIYSLQDEDFDRREGLRSIPARFGAPRALQIARLFHGATLVLF
                     YAVFLAVDGGWLFGGAVVVAGAFLVRQHRLVSPGDLSRVDAAFFTANGWLSVAFLVLG
                     AADVLLR"
BASE COUNT          707 a         1475 c         1764 g          656 t
ORIGIN      
        1 acagctggcg ggagccgccg tcgcccatgc cggagttctt ccgcttgtcg cggcagatct
       61 tgcagcgctt gggctcgttc gagaagccct tctgcgcgta gaactcctgc tcgttggccg
      121 tgaaggtgaa gggggcgccg caatcgatgc aggcgatgga cttgtcggtg tacatacctt
      181 ttctaactcc gtggtctcct ggtctcagga gatcgagcga acgtgggacg ggactccgtg
      241 gcgcacacca tcagagcccg cactcgagga acgtggggaa ctgtttaccg gctccgggac
      301 ttcgggaggc ttcggcgggg tcgtgtcccc tgcgggaccc ctgtgactgg gaatggactg
      361 aaccctcctc aaacgcgata tgtcctggag ccgatcgagg attcctgggg tgggaccaag
      421 gaagaccacg ggctttcgcg gactttagac gggtgccgca agcgatttca agcggaaagt
      481 gacttcccgc ctcaccgaac gccggccggg agggtatgcg gcatctcggc gtgcttcgtt
      541 tctgagattc ggtttctgct gtcgacgaag acgaggtgcg ggcggtggtg aggggcctcc
      601 tcgggcgtca tctcgcagta ggcggccagg atgacgaggt ctcccgggcc tgcgaggtgg
      661 gccgccgccc cgttgatctc gatcgccccg gagccggcct ccccctcgat ggcgtaggtg
      721 gcgagtcggg agccgcgggt gacgttgtaa atctccaccc gctcgtacgg gaggatgccg
      781 gccgcttcga ggagggtgcg gtcgatcgtg acggagccct cgtagtcgag gtgagcccgg
      841 gtgacggtgg cccggtggat cttggccttg agcatcgtga gcttcacggt caccctccga
      901 agacgacgtt gtcgatgagg cgcgtccggc cggcgcggac ggccgcggcg aggaggacgg
      961 gacggtcgac cttctcgacg ggccgcatcg tggcggcgtc gacgaggtcg gcggagtcga
     1021 gctcgaggcg cagctcggtg ccgatcgcca ggcggaaggc ggcgaggagc ttctccgcct
     1081 tccgctcgcc gagctcgtgg acgagctgcg tggcgaggag ggcgcggaag agagcgggcg
     1141 cggccttccg ctcctcgggg gagaggtagg cgttgcggga ggacatcgcg aggccgtcgc
     1201 tctcgcgcac cgtcggggca acgaggacct ccaccgggag gccgaggtcg gcgacgaggc
     1261 gcatcacgac ggcgcactgc tgcgcgtcct tctggccgaa gacggccacg tcggggagga
     1321 cgaggccgag gagccgggcg acgacggtgg cgacgccgtc gaagtgcccg gggcggcgcg
     1381 ccccctccat cccctcggag acgcccgcga cgtggaccgt cgtcgagaag ccgggcgggt
     1441 acatcacgtc gaccgccggg gcgaagagga ggtcggctcc cgcctcgcgg agcttctcgg
     1501 cgtcggccgc aaggtcgcgc gggtagcgct cgaagtcctc ccccgggccg aactgcttcg
     1561 ggttgacgaa gatcgagacg acgaccttcc cggcggcgcg gcgggcctgc tcgacgaggg
     1621 cgaggtgccc cgcgtgcagg gcgcccatcg tcgggacgaa agcgacgcgc tgccccttct
     1681 cgcggtacga ggcgacggcg gagcggaggt cgggaacggt cgagacggtc ttcatcgccg
     1741 cccctccccc gcggcgtaga gcgcgacggg cggagcctcg cccggctcgg cggcgaacga
     1801 ctcggcctcg gaggggaagg cgccgctcct cacgtcggcg acgtacgcgg cggcggcgtc
     1861 gcggatgacg gcgccgacgt cggcgtagcg gcggacgaag cgggggacac gcgcgccggg
     1921 cgccgcgagg ccgacgaggt cgtggaggac gaggacctgc ccgtcgcagc ccgcgccggc
     1981 gccgatgccg atcgtcggga tcgggatcgc ctcggtgatc cgcgcggcga gctcggcggg
     2041 aacgcactcg aggacgaggg cgaagacgcc ggcgtcggcg agcttcttcg cctcgtcgag
     2101 gaggcggcgg gcctcggtgg cgcggcgccc ctggaccttg aatccgccga gcgcgtttac
     2161 cgactgcggc gtcaggccga ggtgccccat gaccgggatc tcggccgcga ggatcgcctt
     2221 gatcatcggg aggcgctgcg agcccccttc gagcttcacg gcgcggcagc ccccctcgac
     2281 gacgaagcgg gcggcgttgc ggacggactc ctcgggggtc gtgtggtagg tgaggtacgg
     2341 gaggtcgccg acgacgaggg cgcgcttcgc cgatcccgag acggctttcg cgtgcgcgac
     2401 catccggtcg agcgaggcgc cgagcgtgtc ggccgcgccg tagacgacca tctcgatcga
     2461 gtcgcccacg aggaggaggt cgacgccggc cgcgtccagc agcgcggccg tcggcgcgtc
     2521 gtaggcggtg agggcggcga tcttccggcg ccccttgaaa gcccggatcg ccggagcggt
     2581 gaggcggagg ggggcgggcg ttccggggct cgaggctccc gtcgccgttc cggtggttcc
     2641 tcgggtcatg gctgcctccc gcacgggagg cgacgagcgg agccggagcc cgcgcgcgcg
     2701 gcgttacggg agagcctttc tcgcgacggg ggtcggccgt ccctttcggg atcgtccgct
     2761 tcgccgggtt cggctcctct cgcgccttcc gtgttcgccg tcgcggtccc taccgggatc
     2821 cgcgcggcac gtagaactgg gtcccgcgga acggacgggt gatctggcgg atcaggtcct
     2881 ccaggtcgtc cttccgcttc acgaagtcta tctggttcgt gtcgaccacg agaagcggcg
     2941 tctcgcgata gtggtggaag aagtagttgt aggcctcggt gagctgcttc aggtagtcgg
     3001 ccgtgatcga cttctcgaac gagcggcccc ggagcttgat gcgcgcgagg cacgtctcca
     3061 ccgtggccgt caggtggatg acgaggtccg gcttcggaag cgcttcggaa agcggctgcc
     3121 agaccttctc gtagaggcgc atctcctcgt cggagagcgt gaggttcgcg aagatcttgt
     3181 ccttcgcgaa cgtgtagtcc gcgacgacga gctccttgaa gaggtccatc tgcgcgatct
     3241 cctgctgctg gccgtagcgg gagagcagga agtagatctg cgcctggaac gcggccccct
     3301 tcttcccctc gtagaagtcg gggaggaagg ggttcttcac gtcttccagg aactgcgtcc
     3361 cggagaagcg cttcgcgagg agcccggcga gcgaggtctt cccgacgccg atcggtccct
     3421 cgatcgcgat gtgcctcagc gggaactttt cggcgttcgg ggcgcccacg gagccgagag
     3481 gttaccgcag gaggacgtcg gccgcgccga gaacgaggaa ggcgaccgag agccagccgt
     3541 tcgccgtgaa gaaggcggcg tcgacccggg agaggtcccc cggggagacg aggcggtgct
     3601 gccgcacgag gaaggcgccc gccacgacga ccgctccgcc gaagagccaa ccgccgtcga
     3661 cggccaggaa gacggcgtag aagaggacga gcgtcgctcc gtggaagagg cgcgcgatct
     3721 ggagcgcccg cggcgcgccg aaccgggccg gaatggagcg gagcccctcc cgccggtcga
     3781 agtcctcgtc ctggaggctg tagatcacgt cgaagccggc gacccagaag acgaccgcga
     3841 ggccgaggag gacgggaggg agggcgaacg ctcccgtcac ggccacccag gccccgaccg
     3901 gcgcgatcgc gagcgagagg ccgaggacga ggtgcgccgc ggccgtgaaa cgcttcgcgt
     3961 aggagtaccc gaggacgacc gcgagggcga cgggcgaaag gtagaggcag aggcgattga
     4021 gctgcgcggc ggcgacgacg aagagcgcgg ccgaggcggc gcagaagagc gcggcggccg
     4081 cgggcgagac gacgcccgcg gggatctcgc ggcgggaagt tcgcgggttc ttcgcgtcga
     4141 cgtgccggtc gacgatccgg ttgaacgaca tcgccgccga gcgggcgccc accatggcca
     4201 agaggatcca gaggagcgtc cgcccgtccg gcacgccgcc cgccgcgagg atcgccgaga
     4261 ggagcgcgaa cgggagcgcg aagacggtgt gggcgaagac gacgagggag aggaagtccc
     4321 gcgggctcgg catcggcgcg cgagtctaaa ccgccggacg gcgccctccg ccgaggcgcc
     4381 ccgccccggg gatgcctcct cgatggggcc acccgcgccg gtgccatctc gcgtcggtgc
     4441 ccccgtcggt gccacccccg atagccccgc cgatggtgcc accccgatgg tgccaggcgt
     4501 gaaatcgtgt attgttaaaa acaatacacg atttcacgcc tggcactatt tccgggagaa
     4561 aaagaggggg gcggttgcac cggcccgcgc aaccgccccg tg
//
LOCUS       DAOTEZ010000353         4599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_13640_length_4599_cov_5.897447, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000353 DAOTEZ010000000
VERSION     DAOTEZ010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13640_length_4599_cov_5.897447"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..933
                     /locus_tag="P5164_15620"
     CDS             43..933
                     /locus_tag="P5164_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY45366.1"
                     /translation="MIEARGLEKRFGAHAALRGVDLRIAEGELFALIGPDGAGKTTFF
                     RLVAGLLTPTAGTLRRAEGATFGLVPQRFSLYEDLTVDENLDLRARLYSVPSAEARAR
                     AAELLRRVGMAPFGARLAGALSGGMKQKLALVAALLTKPRLLLLDEPTTGVDPVSRRE
                     FWLVLNELHHEGLTIVVSTPYMDEAEYATRLGFLDEGKLLAVGTRDEVLAAFPRPILE
                     VLSPDRNGVRRRLAGIDAVDDVSLFGTRLHLRGAAGTGTEILDSVRAGLTGLVPADGV
                     RLVPPTLEDVFVLAGDEVET"
     gene            930..1922
                     /locus_tag="P5164_15625"
     CDS             930..1922
                     /locus_tag="P5164_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007950659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY45367.1"
                     /translation="MRAETSSRAPLPPGVFLRAEGLVKKFGDFVAVNGVTFDVPRGKV
                     FGFLGPNGSGKSTTIRMLAGLLNPTSGTARGFDGLDVTDTERWKSRLGYMSQKFSLYL
                     DLTVEENLRFFGRVYGLAGPRLDERIRELSGTLRFERLKDTLTESLSTGMRQRVALAA
                     ALLHEPLLLFLDEPTGGVDPKGRRLFWELIYDLAAEKGMTVLVTTHYMDEAEQCDELA
                     FILNGDLIAHGEPHALKRGISGRLLEVDLESAPFEALAAVRGAAPLEDAYLFGLRLRV
                     LAREGRVDDALSLVSRLGTPAVAEPSLEDVFVDLARGYAERKAAAQPPTRMVTT"
     gene            1919..3034
                     /locus_tag="P5164_15630"
     CDS             1919..3034
                     /locus_tag="P5164_15630"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY45368.1"
                     /translation="MNRLLALMRKEFLQLRRDTITLRMMVFIPVMQTFIFGYAINYDV
                     KHLKTIVLDEDRSFDSRELVAKMAASQYFDVVGTVGSFEELRREMDEGRVSSALVIDR
                     DYGESRRRGAPARALLIVNASDTTTSTQAMSIAAGISNQLSVQTLARRAGWKEAALPV
                     DLRVRPWYNPDLRTATFIIPGLIAIILTFTLIPFTAIAIVRERERGTLEQLQVTPVTR
                     LEIILGKLFPFILIGVFQLTLIIGLMVFLFRVPIAGSVVQLYVSGLVFMAAVLGLGML
                     VSTVARTQMQAMQLSFVFLLPFVFLSGYVFPIDGMPKVFQLITYVIPARYFIEVIRGI
                     VLRGASLPELWKPMAWMTAYTVLIVGAAVLRFKKTND"
     gene            complement(3158..4405)
                     /locus_tag="P5164_15635"
     CDS             complement(3158..4405)
                     /locus_tag="P5164_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HRY45369.1"
                     /translation="MLDLDAIRAKFPALGSGDVVFDNPGGTQVPREVTDRIVRYLHET
                     NANHGGAFPTSHASDAVVAEGRAAVADFLGAARPEEIAFGPNMTTLTFAMARSLSRGW
                     KPGDEVVLTRLDHDANVSPWLLAAADRGATVRWLDFDPADCTWSVGELRKLLSERTKL
                     VAVGWASNSVGTVNDVAGAAKAAHEAGALVYVDAVHYAPHGPIDVAEAGIDFLACSAY
                     KFFGPHLGILWGRHDLLERTFAYKVRPAHDAPPDRWETGTQSFEAVAGTLGALEYLGW
                     LGERSGGPYREALSGRYAGRRLVLKSAMAAIHAYEARLSRALGEALAAVPGLSVRGIS
                     DPALFAARVPTFSFTMEGRHPRAIAEELGRRGFRVWDGNYYAVGVTERLGLEASGGMV
                     RVGPVHYNTVDEIRRLGAALRSL"
BASE COUNT          633 a         1729 c         1529 g          708 t
ORIGIN      
        1 agccgccggc ggtcccgtcg gcttcccttc gctgagacgg cgatgatcga agcccgcggc
       61 ctcgagaagc gcttcggagc gcacgccgcc ctccggggcg tcgacctgcg catcgccgaa
      121 ggggagctct tcgcgctcat cggtcccgac ggcgccggga agacgacgtt cttccgcctc
      181 gtcgcggggc tcctcacccc gacggcgggg acgctccggc gcgcggaagg agccaccttc
      241 ggcctcgtcc cgcagcgctt ctcgctctac gaggacctga ccgtcgacga gaacctcgac
      301 ctgcgcgccc gtctctactc cgtgccgtcc gccgaggcgc gcgcccgcgc cgcggagctg
      361 ctccgccgcg tcgggatggc ccctttcggc gcccgcctcg ccggcgccct ctcgggcggg
      421 atgaagcaga agctcgccct cgttgccgcg cttctcacga agccgcggct cctcctcctc
      481 gacgagccga cgaccggggt cgatcccgtc tcccgacgcg agttctggct cgtcctgaac
      541 gagctccatc acgaagggct gacgatcgtc gtctcgacgc cgtacatgga cgaggccgag
      601 tacgcgaccc gcctcgggtt cctcgacgag ggaaagctcc tcgcggtcgg cacccgcgac
      661 gaggtcctcg cggccttccc ccggccgatc ctcgaggtcc tctcgcccga ccggaacgga
      721 gtccggcggc gcctcgccgg aatcgacgcc gtcgacgacg tctccctctt cggcacgcgg
      781 ctccacctgc ggggcgccgc cggcaccggg acggagatcc tcgactccgt gcgcgccggc
      841 ctcacgggcc tcgtccctgc ggacggggtc cggctcgtcc cgccgacgct ggaagacgtg
      901 ttcgtgctgg ctggcgacga ggtcgagacg tgagagccga gacctcctcc cgcgcgccgc
      961 ttcctccggg ggtcttcctc cgtgccgaag gtctcgtcaa gaagttcggg gacttcgtcg
     1021 ccgtgaacgg cgtgacgttc gacgtgccgc gcgggaaggt cttcgggttc ctcggcccga
     1081 acggctccgg caagtcgacc acgatccgga tgctcgccgg gctcctcaac ccgacgtccg
     1141 ggacggcccg cggcttcgac ggcctcgacg tgaccgacac cgagcgctgg aagagccgcc
     1201 ttggctacat gagccagaag ttctcgctct acctcgacct gaccgtggag gagaacctcc
     1261 gcttcttcgg gcgcgtgtac gggctcgcgg gcccccggct cgacgagcgg atccgggagc
     1321 tctccgggac gctccggttc gagcgcctga aggacaccct cacggaatcg ctctccaccg
     1381 gcatgcgcca gcgcgtcgcg ctcgcggccg cgctcctcca cgagccgctc ctcctcttcc
     1441 tcgacgagcc gaccggcggc gtcgacccga agggccggcg gctcttctgg gagctcatct
     1501 acgacctcgc cgccgagaag gggatgacgg tcctcgtgac gacccactac atggacgagg
     1561 ccgagcagtg cgacgagctc gccttcatcc tcaacggcga cctgatcgcc cacggcgagc
     1621 cccacgcgct caagcgcggc atctccgggc ggcttctcga ggtcgacctc gagagcgccc
     1681 ccttcgaggc gctcgccgcc gtccgcgggg cagcgcctct cgaagacgcc tacctcttcg
     1741 gcctgaggct ccgcgtcctc gcccgcgagg ggcgcgtcga cgacgccctc tccctcgtct
     1801 cgcggctcgg caccccggcg gtcgccgagc cctcgctgga agacgtcttc gtcgacctcg
     1861 cccgcgggta cgccgagcgg aaggccgcag cccagccgcc gacaaggatg gtgacgacgt
     1921 gaaccgcctc ctcgccctga tgcgcaagga gttcctccag ctccggaggg acacgatcac
     1981 tctccggatg atggtcttca tccccgtgat gcagacgttc atcttcggct acgcgatcaa
     2041 ctacgacgtc aagcacctga agacgatcgt cctcgacgag gaccgctcct tcgacagccg
     2101 cgagctcgtc gcgaagatgg cggccagcca gtacttcgac gtcgtcggga ccgtcggctc
     2161 gttcgaggag ctgcggcgcg agatggacga gggccgggtc tcgagcgccc tcgtcatcga
     2221 ccgggattac ggcgagagcc gtcgacgggg agccccggcg cgggcgctcc tcatcgtgaa
     2281 cgcctccgac acgacgacct cgacccaggc gatgtcgatc gccgccggca tctcgaacca
     2341 gctctccgtc cagacgctcg cccgcagggc cggctggaag gaggccgcgc tccccgtcga
     2401 cctccgcgtg cgcccctggt acaacccgga cctccggacc gcgacgttca tcatccccgg
     2461 gctgatcgcg atcatcctga ccttcacgct catcccgttc accgcgatcg ccatcgtccg
     2521 cgagcgggag cgcggcactc tcgagcagct ccaggtcacg ccggtgacgc gcctcgagat
     2581 catcctcgga aagctctttc ccttcatcct gatcggcgtc ttccagctga cgctgatcat
     2641 cggcctgatg gtcttcctct tccgggtccc gatcgccgga agcgtcgtcc agctctacgt
     2701 ctccggcctc gtcttcatgg cggccgtcct cggcctcggc atgctcgtct ccaccgtcgc
     2761 gaggacgcag atgcaggcga tgcagctctc gttcgtcttc ctcctcccgt tcgtcttcct
     2821 ctcgggctac gtcttcccga tcgacggaat gccgaaggtc ttccagctga tcacctacgt
     2881 catcccggcc cgctacttca tcgaggtgat ccgcgggatc gtcctccgcg gcgcgagcct
     2941 ccccgagctc tggaagccga tggcctggat gacggcctac acggtcctca tcgtcggcgc
     3001 tgccgtcctc cgcttcaaga agacgaacga ctagcccagg agcccacggg tcccgctcct
     3061 ggggtcatcc cgctcgtggg gtcaggtcta ccatctacac tctgcgggcc cgcagagtgt
     3121 agatggtaga cctgacccca cccttgggtc ccttccctca gaggctccgt agggcggctc
     3181 cgaggcggcg gatctcgtcg acggtgttgt agtggacggg gccgacgcgg accatgccgc
     3241 cggaggcttc gaggccgagc cgctccgtca cgccgaccgc gtagtagttg ccgtcccaga
     3301 ctcggaagcc gcggcggccc agctcctcgg cgatcgcccg cgggtgacga ccctccatcg
     3361 tgaacgagaa cgtggggacg cgcgcggcga agagcgccgg gtcggagatc ccgcggaccg
     3421 agaggccggg gaccgcggcg agcgcctccc cgagcgcgcg ggagagtcgc gcctcgtacg
     3481 cgtgaatggc cgccatggcg cttttcagga cgaggcggcg accggcgtag cgaccggaga
     3541 gcgcctcgcg gtacggccct ccggagcgct cccccagcca gccgaggtac tcgagcgcgc
     3601 cgagcgtccc ggcgaccgcc tcgaagctct gcgtccccgt ctcccagcgg tccggcgggg
     3661 cgtcatgagc ggggcggacc ttgtaggcga acgtccgctc gaggaggtcg tggcgccccc
     3721 agaggatccc gagatgcggg ccgaagaact tgtaggccga gcaggcgagg aagtcgatcc
     3781 ccgcttccgc gacgtcgatg gggccgtgcg gcgcgtagtg gacggcgtcg acgtagacga
     3841 gcgccccggc ctcgtgcgcc gccttcgccg ccccggccac gtcgttcacc gtcccgaccg
     3901 agttcgaggc ccagccgacg gcgacgagct tcgtccgctc cgacaggagc ttccggagct
     3961 cgccgacgct ccaggtgcag tcggcagggt cgaagtcgag ccagcgcacc gtcgcgccgc
     4021 ggtccgcggc cgcgagaagc cagggcgaga cgttcgcgtc gtggtcgagg cgcgtcagga
     4081 cgacctcgtc gcccggcttc cagccgcgcg agaggctccg cgccatcgcg aacgtcagag
     4141 tcgtcatgtt cgggccgaac gcgatctcct cgggccgcgc cgccccgagg aagtcggcga
     4201 ccgccgcccg cccctcggcg acgacggcgt cggaggcgtg gctcgtcggg aacgccccgc
     4261 cgtggttcgc gttcgtctcg tggaggtagc ggacgatccg gtccgtcacc tcgcgcggga
     4321 cctgcgtgcc gcccgggttg tcgaagacga cgtctcccga gccgagggcg gggaacttcg
     4381 cgcggatcgc atcgaggtcg agcatgggga aaggttaccg cggagcggtc agcggcggtg
     4441 ccgctcccgc cggagcctct cgcgcagctc gcggagctcg tcgaggcggc gcttcgcctc
     4501 ggcctcccgc cccgtcccct tcggccggta gtagcggcgg ccggccagcg cgtcggggag
     4561 gcacgagaga ccggccgtcc cctccgccgt gtcgtgggc
//
LOCUS       DAOTEZ010000354         4591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_13676_length_4591_cov_4.895062, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000354 DAOTEZ010000000
VERSION     DAOTEZ010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13676_length_4591_cov_4.895062"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="P5164_15640"
     CDS             complement(<1..778)
                     /locus_tag="P5164_15640"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY45370.1"
                     /translation="MAASPPRTHFLRTLGIAGWDALDPVFLAAVATESPLLLVGPHGS
                     AKTMVLNRLAETLGLEHRHYNASLLSFDDLVGFPVPENGRVVYLQTPATVWEAESVLV
                     DEVSRCRPEVQNKLFPLVHERVIQGLPLPRLRHRWAAMNPPASLEGDDAGDTAYSGAE
                     PLDVALADRFAFVVTVPSLAGLSPDDQRRVLRPSAWSADEARPVVARLVEEARAGLTP
                     HSRGEAAEAAAATEYSMLLAAQLARAGHPLSTRRAAQVVRN"
     gene            complement(1047..1451)
                     /locus_tag="P5164_15645"
     CDS             complement(1047..1451)
                     /locus_tag="P5164_15645"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRY45371.1"
                     /translation="MIHVDTSFLVDLMREAGRGQEGPATRFLASRPDEVLGASVFVLC
                     ELAAGAELARDPARERERVARLAESLETVSPEGDFAATYGRLLARLQRGGVKVATMDL
                     LIGTAAIEAGAPLLTRNRKDFERIPGLEVLTY"
     gene            complement(1448..1681)
                     /locus_tag="P5164_15650"
     CDS             complement(1448..1681)
                     /locus_tag="P5164_15650"
                     /inference="COORDINATES: protein motif:HMM:NF014723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VapB family protein"
                     /protein_id="HRY45372.1"
                     /translation="MAVKTITVDLEAYELLARRKRPGMSFSQVIKLHLGPRGTARDLL
                     AMTKAAPLAPKTLDAVDGLIRGRRASPARAPKL"
     gene            complement(1825..3090)
                     /locus_tag="P5164_15655"
     CDS             complement(1825..3090)
                     /locus_tag="P5164_15655"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRY45373.1"
                     /translation="MAGWLVPLRDLTPAQLRAVELPADGHRVLSGGPGSGKTLVLLHR
                     ARRLADSLRIPEGRLLVLVYTNALTAFLRSALAELGIPESAVRTFDDWCAAHYERFVS
                     AEKPWDERRGRPRFDAIRDGVLREVRRHPDRPPLYDAVLVDEGQDLDAHAFETLAAVS
                     RHLTVALDPGQRIYDGGAREEEILAALRIPSRSVALLDAWRCSPCVSRLASRLLPEGE
                     ARQAFLRNAPTAAVEREKPLLYLARDPSDEIARLADVSRARLLRGERVAVLLPGRRLV
                     ASIACGLSRHGIPVEVPHRPGTRPGPFPPLDFASDLVKVMPYHSAKGLTFDSVLLPRL
                     STSDVLVRGASSLLRLLFVGITRATSWVYLSAVEGDELPLLRRLAAGAPEVVVQRHGA
                     HGAPPAAPGGTGSEPGAPAGPGAGLTDWL"
     gene            complement(3102..>4591)
                     /locus_tag="P5164_15660"
     CDS             complement(3102..>4591)
                     /locus_tag="P5164_15660"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRY45374.1"
                     /translation="SEIRRLRGLGRWEAADPNEVAKTLPGSYPCRLPDGRDLRLTSPR
                     LTARQLDGVLFPFLDEELLYAKGDEYRTTCSIFAPIEDALSRAGLSADQIDGALLVGG
                     SSLIPQVVDSVARRFPTARVFREETPEAVQSSVARGAALHALSLAASRRGLLTPVTGN
                     ALSVRTASGLVELVARGTPLPFPASGGFAACTELAVPESRTREPLPLRVELRDGAGGP
                     VLNALCLFPPPVRQGEPLRLRYRVDANQLLHLRVDLPARPGAGGHELRLENPLTNVVN
                     SQPKRERLLEIEESLRTGLLSGSKAAAATEEAARLYADLGQREKALSVYRALLRAKGR
                     PDADLLFRMGLLAGELGDDGREERFYLESAEADPTDSEALFNLALARRRRELLPEALT
                     AVTEAIRRDPDAAYLVLRALVHDGLGDVPDRDADLARAFATFAPLPTQGDFALAWYLA
                     ACRLAGDAPRLAAAEAEEKRRRTTKPGLPFVDGLLPVLAAFAQTN"
BASE COUNT          617 a         1428 c         2015 g          531 t
ORIGIN      
        1 cgttcctcac gacctgcgcg gcccggcgcg tggagagcgg gtggccggcg cgggcgagtt
       61 gcgcggcgag gagcatcgag tactcggtgg cggcagccgc ttcggccgcc tcgccgcggg
      121 agtgcggggt gaggcccgcg cgtgcctcct cgacgaggcg cgccaccacg gggcgggcct
      181 cgtcggcgct ccaggccgac gggcggagga cgcggcgctg gtcgtcgggc gagaggccgg
      241 cgagggaggg gacggtgacg acgaaggcga agcggtcggc gagcgcgacg tcgagcggct
      301 cggcgccgga gtaggcggta tcgcccgcgt cgtccccctc gagcgaggcg ggcgggttca
      361 tggcggccca gcggtggcgg aggcgcggga gcgggagccc ctggatgacc cgctcgtgga
      421 cgagggggaa gagcttgttc tgcacctcgg ggcggcagcg cgagacctcg tcgacgagga
      481 ccgactcggc ctcccagacg gtggcgggcg tctggaggta gacgacgcgg ccgttctcgg
      541 ggacggggaa gccgacgagg tcgtcgaagg agaggagcga ggcgttgtag tggcggtgct
      601 cgaggccgag ggtctcggcg aggcggttga gcaccatcgt ctttgccgaa ccgtgcggcc
      661 cgacgaggag gaggggggac tccgtcgcga ccgcggcgag gaagaccggg tcgagcgcgt
      721 cccacccggc gatgccgagc gtgcggagga agtgcgttcg cggaggagat gcggccatgg
      781 gcgccctttc gacgaggaga gttcccgcct cgatcgcgca tgggtcccga cctttcggcc
      841 gggccggctg ggccaccgtg taggactacg gtgtggcgtg cgggtcgtcg agccggtgct
      901 ctcgccgcat ctcttcatgc cggcagcccg aagcggaggg ctgcctgagg ttcaaagaac
      961 ggcgccggaa acgggggccc ggcgcgaggg agattttctc acgggagggc gggggggaga
     1021 aggcgggggg cgaagcccgg agagcctcag taggtgagca cctcgaggcc ggggatccgc
     1081 tcgaagtcct tcctgttgcg agtcaggagc ggcgcgccgg cctcgatggc ggccgtcccg
     1141 atgaggaggt ccatcgtcgc gaccttcacg cctccgcgct ggagccgcgc gagcaggcgg
     1201 ccgtacgtgg ctgcgaaatc gccctccggg ctcaccgtct cgagcgactc ggccagccgc
     1261 gcgacacgct cgcgctcccg cgccgggtcg cgagccagct cggcgcccgc ggccagctcg
     1321 cagaggacga agaccgacgc cccgaggacc tcgtcgggcc gggacgcgag gaaccgcgtc
     1381 gcggggcctt cctggcctcg cccggcttcc cgcatcaggt cgacgaggaa ggaggtgtcg
     1441 acgtgaatca cagcttcggg gcccgtgccg gggaggccct ccggcctcgg atcagcccgt
     1501 ccaccgcgtc gagcgtcttc ggcgctagcg gcgcagcctt cgtcatcgcg aggaggtccc
     1561 gggccgtccc gcgcgggccg aggtggagct tgatgacctg cgagaacgac atcccggggc
     1621 gcttgcggcg cgccaggagc tcgtacgcct cgaggtccac cgtgatcgtc ttgacggcca
     1681 tgcacggatc atgcacggac gggccgcggg cggacaaggg cccgtgagtc cggtcggcga
     1741 ggacatggac cgactgccgc tcactccgcg aaggtcctcc tcgcggcgag gaagaccggg
     1801 tcgggcgcgt ccgaggcgtt cccctcagag ccagtcggtc aagcccgcac ccgggcccgc
     1861 gggcgcgccc ggttcactgc ccgtgccgcc cggcgcggcg ggcggggctc cgtgcgcgcc
     1921 gtggcgctgg acgacgacct ccggcgcgcc ggccgcgagg cggcggagga gcggcagctc
     1981 gtctccctcg accgccgaga ggtagaccca cgaggtcgcg cgggtgatgc cgacgaagag
     2041 gagccggagg agggaggagg cgccccggac gaggacgtcg gaagtcgaga ggcgggggag
     2101 gaggacggag tcgaaggtga ggcccttcgc gctgtggtac ggcatcacct tgacgaggtc
     2161 ggaggcgaag tcgagcggcg ggaaggggcc agggcgcgtg ccggggcggt gcgggacctc
     2221 gacggggatg ccgtgtcggg agaggccgca ggcgatcgag gcgacgaggc ggcggcccgg
     2281 gaggaggacg gcgacgcgct cgccgcggag gagccgcgcg cgggagacgt cggcgaggcg
     2341 ggcgatctcg tcggaggggt cgcgggcgag gtagaggagc ggcttctcgc gctcgaccgc
     2401 cgcggtgggc gcgttccgaa ggaaggcctg gcgcgcctcg ccctcgggga ggaggcggga
     2461 ggcgaggcgc gagacgcagg gcgagcagcg ccaggcgtcg aggagggcga cggagcgcga
     2521 ggggatgcgg agggcggcga ggatctcctc ctcgcgggcg ccgccgtcgt agatccgctg
     2581 gcccgggtcg agcgcgacgg tgaggtggcg ggagacggcg gcgagggtct cgaaggcgtg
     2641 cgcgtcgagg tcctgcccct cgtcgacgag gacggcgtcg tagagcggcg ggcggtcggg
     2701 gtggcggcgg acctcgcgga ggacgccgtc gcggatggcg tcgaagcggg ggcggccgcg
     2761 ccgctcgtcc cacggcttct cggccgagac gaaccgctcg tagtgcgcgg cgcaccagtc
     2821 gtcgaaggtg cggacggcgc tctcgggaat gccgagctcg gcgagggcgg agcggaggaa
     2881 cgcggtgagg gcgttggtgt agacgaggac gaggagccgg ccctcgggaa tgcggaggga
     2941 gtcggcgagg cggcgggcgc ggtggaggag gacgagcgtc ttgcccgagc cggggccgcc
     3001 ggagaggacg cggtggccgt cggcggggag ctcaacggcc cggagctgcg cgggcgtcag
     3061 gtcgcggagg gggacgagcc agccggccaa ctctgatccg ctcagttcgt ctgcgcgaag
     3121 gcggcgagga cggggaggag gccgtcgacg aacggcaggc ccggcttcgt ggtgcggcgc
     3181 cgcttctcct cggcttcggc cgccgcgagg cggggcgcgt cgcccgcgag gcggcaggcg
     3241 gcgaggtacc aggcgagcgc gaagtcgccc tgcgtgggga gcggggcgaa ggtggcgaag
     3301 gcccgggcga ggtcggcgtc gcggtcggga acgtcgccga ggccgtcgtg gacgagggcg
     3361 cggaggacga ggtacgccgc atccgggtcg cggcggatcg cctcggtcac cgcggtgagc
     3421 gcctcgggca ggagctcccg gcgccttcgg gcgagggcga ggttgaagag ggcctcgctg
     3481 tcggtcgggt cggcctcggc gctctcgagg tagaagcgct cctcgcgccc gtcgtcgccc
     3541 agctcgcccg cgaggagccc catccggaag agaaggtcgg cgtcgggccg gcccttcgcg
     3601 cggaggaggg cgcggtagac ggagagcgcc ttctcgcgct ggccgaggtc ggcgtagagg
     3661 cgggccgcct cctccgtcgc cgcggccgcc ttgctcccgg agaggaggcc ggtgcggagg
     3721 ctctcctcga tctcgaggag gcgctcgcgc ttcggctggg agttgacgac gttcgtgagc
     3781 gggttctcga gccggagctc gtgtccgccg gcgccggggc gcgcggggag gtcgacgcgg
     3841 aggtggagga gctggttcgc gtcgacgcgg tagcgcaggc gcaggggctc gccctggcgg
     3901 acggggggcg ggaagaggca gagggcgttc agcacggggc cgccggcgcc gtcgcgcagc
     3961 tcgacgcgga gcgggagcgg ctcgcgcgtg cgcgactcgg ggacggcgag ctcggtgcag
     4021 gcggcgaagc cgccggaggc ggggaagggg aggggcgtgc cgcgtgcgac gagctcgacg
     4081 aggcccgagg ccgtgcggac ggatagggcg ttgccggtca cgggtgtcag gaggccgcgc
     4141 ctcgaggccg ccagcgagag ggcgtggagc gcggcgccgc gggccacgga cgactgcact
     4201 gcctcgggcg tctcctcgcg gaagacgcgg gcggtgggga agcggcgcgc gacggagtcg
     4261 acgacctgcg ggatgaggct gctgccgccg acgaggagcg ccccgtcgat ctgatcggcc
     4321 gagaggccgg cgcgggagag ggcgtcctcg atcggggcga agatcgagca ggtggtgcgg
     4381 tactcgtctc ccttcgcgta gaggagctct tcgtcgagga acgggaagag gacgccgtcg
     4441 agctggcggg cggtgaggcg ggggctcgtg aggcggaggt cgcggccgtc ggggaggcgg
     4501 caggggtacg agccggggag ggtcttcgcg acctcgttcg ggtcggcggc ctcccagcgg
     4561 ccgaggccgc gcaggcgccg gatctcggag c
//
LOCUS       DAOTEZ010000355         4585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_13706_length_4585_cov_7.191832, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000355 DAOTEZ010000000
VERSION     DAOTEZ010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13706_length_4585_cov_7.191832"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            209..1087
                     /locus_tag="P5164_15665"
     CDS             209..1087
                     /locus_tag="P5164_15665"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HRY45375.1"
                     /translation="MPRRFALALLALLVLLLLGLLAGRKVHESRQKRRVANQPVPTRT
                     IPPPPTPIPARLAAIWFESAADGMFRGEVREVPAAVDDVALLRRLGAEVLAGPRHPDL
                     LRPFPEGWTLRAAFRLADGLAVLDLAPPAAPAPTPVPEETTEAKGAGKGAKEPATPTP
                     TPVASEPPKRWEAGSSEELAAVQSLVLTVTKNLPAVTRVILLVGGEPVETLAGHVDLT
                     RPLAPDLSRAADEAPLVAPMPTPLPEPTAPAEPAASPSPGATAPSTVPAPAVSPDPPR
                     AAARPTPPLRKSLDRT"
     gene            1084..1905
                     /gene="murI"
                     /locus_tag="P5164_15670"
     CDS             1084..1905
                     /gene="murI"
                     /locus_tag="P5164_15670"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRY45376.1"
                     /translation="MTDPRPIGLFDSGVGGLTVLDAVARRLPGEDLLYLGDTARLPYG
                     TKSPETVRRYARRAVSLLLSRGVKALVVACNTASALALDAIVDEARGVPVLGVVEPGA
                     RAALRTARGVTGPAAGAPIAVLATEATVASDAYGRAIRSLDPSRAVMNVACPLFVPLA
                     EEGWTENEVAREAAKLYLAPVVAAGARVVVLGCTHYPLLARTIAAALPGGTLLVDSAA
                     STAVEVEALLGTEGRRAGEAGETHLLVTDSSERLRRVAGRFLGREAAALELVDLP"
     gene            1917..2687
                     /gene="rph"
                     /locus_tag="P5164_15675"
     CDS             1917..2687
                     /gene="rph"
                     /locus_tag="P5164_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HRY45377.1"
                     /translation="MSRPDGRSPLQLRPVSLEVGVVPHAEGSCLITQGRTKVLIAATL
                     EQKIPPFLRDSGEGWITAEYGMLPRATLERTPREAAKGKQAGRTLEIQRLIGRALRGV
                     ADRTTFPEWTVTLDCDVLVADAGTRCASVTGAWVALALALESLRKKSALKRPRPFREA
                     VAAISVGVLPAPERPEGVVALDLDYSEDSTAWVDLNVVGTSTGRYVEVQGTAEREPFD
                     DRRLREMLVVGKKGLRRLFRAQRKALLGRVPEDWLPPA"
     gene            complement(2724..3479)
                     /locus_tag="P5164_15680"
     CDS             complement(2724..3479)
                     /locus_tag="P5164_15680"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY45378.1"
                     /translation="MSRTLLLLHGLGGSAEDWKEVAAALPKGLEALAVDFPGFGKSAR
                     PHDSYDPAALARWVIGEMDDHDVETAVVAGHSLGGRVAAEVALLAPKRVAALALVSPL
                     GAVSYGFAETLKWKAMSRAALVAAAPETSLKNALGYGFAVDGAGKKGFVARGMKARTG
                     ANAAAALRALERSVDGLLSSPPLRKRLDGTTVPLLVATGAEDSLAPPRDVKAILEARS
                     DARYVELPGVGHYAPLEAPKAVAKLFAELAKKG"
     gene            3535..3609
                     /locus_tag="P5164_15685"
     tRNA            3535..3609
                     /locus_tag="P5164_15685"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3567..3569,aa:Pro,seq:tgg)
     gene            3851..4099
                     /locus_tag="P5164_15690"
     CDS             3851..4099
                     /locus_tag="P5164_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRY45379.1"
                     /translation="MNEIAARIDTSGRLVIPARIRKDLGLTAGTAVTLRVSDGELRVV
                     AKAEAIRAAQARVRRYVKPGRSLAGELIAERRAEWRRG"
     gene            4092..4475
                     /locus_tag="P5164_15695"
     CDS             4092..4475
                     /locus_tag="P5164_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015148888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRY45380.1"
                     /translation="MGKVVLDASAVLALLNDEPGAAAVAAALEDAVVNAVNLCEVVAK
                     LQEVGMSREEAEEALGGLGLEVQPFGEAAAWAAGTLRGATRRAGLSLGDRACLALARE
                     LGVPAITADAAWLKAATGADVRLVR"
BASE COUNT          525 a         1878 c         1595 g          587 t
ORIGIN      
        1 tgtcccactg gataatcccg ttttcattaa gtttttgaag caaaacctgc tggcgataaa
       61 attcgggacg atcgttatcc tgggcttatt ggcggcgctg ctgtcggcct tcctcggaaa
      121 attatccggg catccccgcg ccggagcgac gccgtcgtcc ccgttccgtt cgcgccgccc
      181 gcagccgagc ggcgctgacg ccggtcccat gccgcgccgc ttcgccctcg cgctcctcgc
      241 gctcctcgtc ctcctcctcc tcggcctcct cgccgggcgg aaggtccacg agtcgcggca
      301 gaagcggcgg gtcgcgaacc agcccgttcc gacccggacg atcccgccgc ccccgacgcc
      361 gattcccgcg cgcctcgcgg cgatctggtt cgagtcggcg gccgacggga tgttccgggg
      421 ggaggtgcgc gaggtgcccg cggccgtcga cgacgtcgcg ctcctccgcc gcctcggcgc
      481 cgaggtcctc gccgggccgc ggcaccccga cctcctccgc cccttcccgg aaggctggac
      541 gctccgcgcc gccttccgcc tcgccgacgg cctcgccgtc ctcgacctcg cgcctccggc
      601 ggcccccgcg ccgacgcccg ttcccgagga gacgacggag gcgaagggcg ccggcaaggg
      661 cgcgaaggag cctgcgacgc cgaccccgac gccggtcgca tccgagccgc cgaagcgctg
      721 ggaggccggg tcctccgagg agctcgccgc ggtgcagtcg ctcgtcctca ccgtcacgaa
      781 gaacctcccc gccgtcaccc gcgtcatcct cctcgtcggc ggggagccgg tggagacgct
      841 cgccgggcac gtcgacctga cgcgcccgct cgctccggac ctctcccgcg cggccgacga
      901 ggcgccgctc gtcgccccga tgccgacgcc gctgccggag ccgaccgcac cggccgagcc
      961 ggccgcctct ccttcccccg gcgcgaccgc gccttcgacc gtcccggcgc ccgcggtctc
     1021 gcccgatccg ccgcgcgccg ccgcgcgccc gactccacct ctccgaaaga gcctcgaccg
     1081 cacatgacag acccgcgtcc catcggcctc ttcgactccg gcgtcggagg gctcaccgtc
     1141 ctcgacgccg tcgcccgccg cctccccggc gaagacctcc tctacctcgg cgacaccgcg
     1201 cgcctcccct acggcacgaa gtcgcccgag accgtccgcc gctacgcgcg gcgcgccgtc
     1261 tcgcttctcc tgtcgcgcgg ggtcaaggcg ctcgtcgtcg cgtgcaacac cgcctcggcc
     1321 ctcgcgctcg acgcgatcgt cgacgaggcg cgcggcgtcc cggtcctcgg cgtcgtcgag
     1381 ccgggcgcgc gcgccgcgct ccgcaccgcg cgcggcgtga cgggcccggc cgccggggcg
     1441 ccgatcgccg tcctcgccac cgaggcgacc gtcgcctccg acgcctacgg ccgcgcgatc
     1501 cgctccctcg acccctcccg cgccgtcatg aacgtcgcct gcccgctctt cgtcccgctc
     1561 gccgaggagg ggtggaccga gaacgaggtc gcccgcgagg cggcgaagct ctacctcgcc
     1621 cccgtcgtcg cggcgggggc gcgcgtcgtc gtcctcggct gcacgcacta cccgctcctc
     1681 gcgcggacga tcgccgccgc gctccccggg gggacgctcc tcgtcgactc cgcggcctcg
     1741 accgcggtcg aggtcgaggc gctcctcggc acggaggggc ggcgcgcggg cgaggcgggg
     1801 gagacgcacc tcctcgtcac cgactcctcc gagcgcctcc ggcgcgtcgc gggacgcttc
     1861 ctcggccgcg aggcggcggc gctcgagctc gtcgacctgc cgtaagctcc ggggcgatgt
     1921 ctcgccccga cggccgctct cccctccagc ttcgccccgt ctccctcgag gtcggcgtcg
     1981 tgccgcacgc cgagggctcg tgcctcatca cccagggccg gacgaaggtc ctgatcgccg
     2041 ccacgctcga gcagaagatc ccgcccttcc tgcgcgactc gggcgagggg tggatcaccg
     2101 ccgagtacgg gatgctcccg cgcgccacgc tcgagcggac gccgcgcgag gcggcgaagg
     2161 ggaagcaggc cggacggacg ctcgagatcc agcgcctcat cgggcgcgcc ctccgcggcg
     2221 tcgccgaccg gacgacgttc cccgagtgga ccgtcacgct cgactgcgac gtcctcgtcg
     2281 ccgacgcggg cacgcgctgc gcgtcggtga ccggcgcctg ggtcgccctc gccctcgccc
     2341 tcgagagcct ccggaagaag agcgctctca agcggccgcg cccgttccgc gaggcggtcg
     2401 ccgcgatctc cgtcggcgtc ctccccgccc ccgagcgccc cgagggggtc gtcgccctcg
     2461 acctcgacta ctccgaggac tcgacggcct gggtcgacct gaacgtcgtc ggcacctcca
     2521 ccggacgcta cgtcgaggtg caggggacgg ccgagcgcga gccgttcgac gaccggcgcc
     2581 tccgcgagat gctcgtcgtc ggcaagaagg ggctccgccg cctcttccgc gcccagcgca
     2641 aggcgctcct cggccgcgtt ccggaggact ggctcccgcc ggcgtgaccc cggcggccgc
     2701 ggtccgcgcc gcgggctcgg ccttcagccc ttcttcgcga gctccgcgaa gagcttcgcc
     2761 accgccttcg gcgcctcgag cggcgcgtag tggccgacgc cgggaagctc gacgtaccgc
     2821 gcgtcggagc gcgcctcgag gatcgccttc acgtcgcggg gcggcgcgag cgagtcctcc
     2881 gcgccggtcg ccacgaggag cggcaccgtc gtcccgtcga gccgcttgcg gagcggaggc
     2941 gacgagagga ggccgtcgac cgaacgctcg agcgcccgga gcgccgccgc ggcgttcgcc
     3001 ccggtccgcg ccttcatccc gcgcgccacg aaccccttct ttcccgcgcc gtcgacggcg
     3061 aagccgtagc cgagcgcgtt cttcagcgac gtctccggcg ccgcggcgac gagcgcggcg
     3121 cgcgacatcg ccttccactt cagcgtctcg gcgaagccgt aggagacggc cccgagcggc
     3181 gagacgagcg cgagggccgc gacccgcttc ggcgcgagga gcgccacctc ggccgcgacc
     3241 cgcccgccga gcgagtgccc cgcgacgacg gccgtctcca cgtcgtggtc gtccatctcc
     3301 ccgatgaccc accgcgcgag cgccgccggg tcgtagctgt cgtgcgggcg cgccgacttg
     3361 ccgaagccgg ggaagtcgac cgcgagcgcc tcgagcccct tcggaagcgc cgcggcgacc
     3421 tccttccagt cctcggccga gccgccgagc ccgtgcagca gcaggagcgt ccggctcacg
     3481 ccgcgcctcc cgcgggtgga catcccctcg ccgcggcccg agaatccggc gcgccggggc
     3541 gtagcgcagt ggcagcgcag ctgctttggg agcagcaggt cggcggttca aatccgcccg
     3601 ccccgaccac tccttccctt ccgaacgcgc gatcgctcac gcgcccctcc cgggcgcccg
     3661 cggcgccctg cccgtcgcat cgtccccggc ggctctcacg gttgcgggat cgtctcagat
     3721 ccgaaggccc ggacgccaca ctccgcgggc gggccgacgg gcgacgccgc ccgcccggcc
     3781 cgccccggcc ctgcgaccga caccgcgccc tctcctcgcg cacggcggta gactcggctc
     3841 gaggtggcag ttgaacgaga tcgcggcacg gatcgatact tcgggcagac tcgtcattcc
     3901 ggcccgcatc cggaaggacc tggggctcac ggcggggacg gcggtcacgc tccgggtctc
     3961 ggacggcgag ctgcgcgtcg tggcgaaggc cgaggcgatt cgcgcggcgc aggcgagggt
     4021 ccgccgttac gtgaaacccg ggcgctcgct cgcgggggag ctcatcgccg agcggcgggc
     4081 ggagtggcgg cgtgggtaag gtcgttctcg acgcctcggc ggtcctcgcc ctcctgaacg
     4141 acgagccggg cgccgcggcc gtggccgccg cgctcgagga cgcggtcgtg aacgcggtga
     4201 acctctgcga ggtcgtcgcc aagctccagg aggtcggcat gtcacgggag gaggccgagg
     4261 aggccctcgg cgggctcggc ctcgaagtgc agcccttcgg cgaagcggcc gcctgggccg
     4321 ccggcacgct ccggggggcg acacggcgcg cgggcctctc tctcggcgac cgggcctgtc
     4381 tcgccctcgc gcgggagctg ggcgtccccg cgataacggc cgacgcggcc tggctgaagg
     4441 cggcgaccgg ggcggacgtc cggctcgtcc gctgacccga ggtcctgcgg cggcctcagc
     4501 ggctcgcggc gcggtaccgc cagggcgcga ccgggtcggg ctccgaccag acgccgaccc
     4561 gcgccgtccg cgcctgccgc tccgc
//
LOCUS       DAOTEZ010000356         4581 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_13719_length_4581_cov_6.392179, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000356 DAOTEZ010000000
VERSION     DAOTEZ010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13719_length_4581_cov_6.392179"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="P5164_15700"
     CDS             complement(<1..677)
                     /locus_tag="P5164_15700"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRY45381.1"
                     /translation="MSRPLSVVTGAALILLLGLSGIASAQGTVTGSIQGTVKDAEGGA
                     LPGTLVTTYSSALVKGRMTTTTDARGGWRFPALPPGNYAVEAELQGFKKVRRDGIRVS
                     LGQAMAIDLVLPLTSVAAEAVVTAEAPIVSVVENKVHTSFGGDFIDKQAVPRNYYQVL
                     GSAPGVNIDSSAGQASSSMLAYGGTTSNQNAFTLDGVNVGDAASGNYWLLPSIQWMEE
                     IQVAGLGA"
     gene            complement(791..1516)
                     /locus_tag="P5164_15705"
     CDS             complement(791..1516)
                     /locus_tag="P5164_15705"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRY45382.1"
                     /translation="MTALCRIYNVENIMQARSGPERHRQLRQWVADRLRAEILEGRRR
                     PGDWLRQESVAREEGVSQTPVREALKQLVAEGLLEHVPYRGIRVVTLSVEDAEDLYAS
                     RAVLEPMAARHAARAISPEEVRELSSLHERMIACEIPARLREYRDLNRRFHEAIIAAS
                     RRPFLTRTLGHLWSAFPTMLWSNVPGIATTSSPEREDPDAAEHAEIVAALGAHDPERA
                     AAAVKRHVEEAGRTLVAAMRAQP"
     gene            complement(1552..3549)
                     /locus_tag="P5164_15710"
     CDS             complement(1552..3549)
                     /locus_tag="P5164_15710"
                     /inference="COORDINATES: protein motif:HMM:NF021639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HRY45383.1"
                     /translation="MSALVETVLSASSPEERADAFVRLLRDSPAPRPGANGPRSRIGA
                     ILDALEASPDLRAGFLSALSALFAEGDGTALFGDVGIPSDKGFFAELGGRVSARLIPS
                     PRDAHDFVEIAARLFRTEADVDAFGDLPLSDLHRLVRLFETGLTREAAASLSRAFADG
                     FRLLLARIHAQGLSRELRSRATPGSVSASPFHRLLRSGDALLEAWLCEREVGAVLQAF
                     NKDSGECRKEVRAIQRRLEEAGVSVDVVFGLEVIDRCLTRTALMADVMTLPPGPDRSR
                     ALHRLLSRLVRHAHEDRSVLHLVRWNLHLLDRKIVDRTGKTGEHYVAASRPEYRHIWL
                     AAAGGGIVTVFTAAFKMLVHEWHLAAFPEGFLYGLNYAISFLVMQAFGLILATKQPAM
                     TAAALASILRETKGDDREEKIAGFFARLTSSQLAAALSNVFVVSAGAAVFVAAWSALA
                     GRPWVDEKTARETFFVLSPFDSGTIFFAALTGVLLWLGSVAGGWFENWSTYHRLPEGI
                     ARHPLGRLVGADRMRRVADALARNASGWATNVALGFLLGFAPAIGRFFGIPFDVRHVT
                     LSTGQLGLAASSLGQGWFAEGAFLRGLAGIGVMFVLNLSVAFSLSLFNAARAYEMPPG
                     ELAGVLRHLFGRALRRPLDFVRPPRPVDREKVESPAPDSGH"
     gene            3605..>4581
                     /locus_tag="P5164_15715"
     CDS             3605..>4581
                     /locus_tag="P5164_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HRY45384.1"
                     /translation="MPANLTPPYHDAEERFKAATTHEERLAAMREMIALLPKHKGTEK
                     LYADLKKRLAKLESEVEHAAHGGRRADPGHVKREGAGQWVLLGEPNAGKSSLLAALTH
                     AHPEIGEYPFTTRVPLPGMMPFEDVQVQLVDTPAVAAGHTEPYLTNLAHNADGVLLVL
                     DVAADDGEESAKLLLELLEKARVWPRVRPLPPDAPPFLAVRPVIALGNKRDLDDGTFA
                     EMAREAIGADLPFHPVSALSGEGLDDLRLLLWRELRRIRVYAKEPGKKPDLAAPFVLP
                     DGATVHDLAERVHKDVAAALKFARIWGHAKFDGQQVDRAHVLADRDVVEL"
BASE COUNT          681 a         1547 c         1715 g          638 t
ORIGIN      
        1 gcgccgaggc cggccacctg gatctcctcc atccactgga tgctcgggag gagccagtag
       61 ttgccgctcg ccgcgtcgcc cacgttgacg ccgtcgagcg tgaaggcgtt ctggttcgac
      121 gtcgtcccgc cgtacgcgag catgctgctg ctggcctgac cggccgacga gtcgatgttg
      181 acgcccgggg ccgacccgag gacctggtag tagttccgcg ggacggcctg cttgtcgatg
      241 aagtcgccgc cgaaggaggt gtggaccttg ttctcgacga cgctgacgat cggcgcctcg
      301 gccgtgacga cggcctcggc ggcgacggag gtgagcggaa ggacgaggtc gatggccatc
      361 gcctgtccga ggctcacccg gatcccgtcg cggcggacct tcttgaagcc ctgcagctcg
      421 gcctcgacgg cgtagttgcc gggggggagc gcggggaatc gccagccgcc ccgggcgtcg
      481 gtggtcgtcg tcatgcggcc cttgacgagg gccgacgagt acgtggtgac gagcgtcccc
      541 ggaagggcgc ccccctcggc gtccttgacg gtgccctgga tgctgccggt cacggtgccc
      601 tgggcgctcg cgattcccga gaggcccagc agcaggatca gggcggctcc tgtgacgacg
      661 gagagcggac gtgacatgac tcctcctctt gctcggcctg gggccgtttc gtgacgccag
      721 ggggtgcgag ttccgggtcg accgttcttg gagcggcgcg gggcgcgccc gggagagcgc
      781 gtcgggcgct tcatggctgg gccctcatgg cggcgacgag ggtccgtccc gcctcctcca
      841 cgtgccgctt cacggcggcg gcggctcgtt cgggatcgtg cgcgccgagc gccgcgacga
      901 tctcggcgtg ctcggcggcg tcggggtcct cccgctcggg cgaggaggtc gtggcgatgc
      961 cggggacgtt gctccagagc atcgtcggga acgccgacca gaggtgcccg agagtgcgcg
     1021 tgaggaaggg ccggcgcgag gccgcgatga tcgcctcgtg gaaacggcgg ttcaggtcgc
     1081 ggtactcgcg gaggcgcgcg gggatctcgc aggcgatcat ccgctcgtgg agcgaggaga
     1141 gctcgcggac ttcctcgggc gagatcgccc gggccgcgtg gcgtgccgcc atcggctcga
     1201 ggacggcgcg gctcgcgtag aggtcctcgg cgtcctcgac ggaaagcgtg acgacgcgga
     1261 tgccgcgata gggaacgtgc tcgaggagcc cctcggcgac gagctgcttg agcgcctcgc
     1321 gaacgggcgt ctggctgacg ccctcttcgc gcgcgacgct ctcctgtcga agccagtccc
     1381 ccggcctgcg gcggccctcg aggatctcgg cccggagcct gtcggcgacc cactggcgga
     1441 gctgccggtg tcgctcgggt cccgatcgcg cctgcatgat gttctctaca ttatatattc
     1501 tgcatagcgc tgtcacgaac tttctatcca attgatctgt agtggtttgt ctcagtgccc
     1561 cgagtccggg gcgggcgatt cgaccttttc ccggtccacc ggtcgcggtg gccggacgaa
     1621 gtccagggga cgcctcaggg ccctgccgaa gaggtgcctc agcaccccgg cgagctcccc
     1681 gggcggcatc tcgtaggccc gcgccgcgtt gaagagcgac agcgagaacg ccaccgagag
     1741 gttcaggacg aacatcacgc cgatcccggc gaggccgcgc aggaacgccc cttcggcgaa
     1801 ccagccctgc ccgaggctcg aggccgcgag cccgagctgt cccgtcgaga gcgtgacgtg
     1861 ccggacgtcg aaggggatgc cgaagaagcg gccgatcgcg ggggcgaacc cgagcaggaa
     1921 cccgagcgcg acgttcgtcg cccagcccga ggcgttccgc gcgagggcgt cggcgacgcg
     1981 ccgcatccgg tcggctccga cgaggcggcc gagcgggtgg cgcgcgatcc cctcgggcag
     2041 ccggtggtag gtcgaccagt tctcgaacca gccccccgcg acgctgccga gccagaggag
     2101 gacccccgtc agcgcggcga agaagatcgt cccgctgtcg aacgggctga ggacgaagaa
     2161 ggtctcccgc gccgtcttct cgtcgaccca ggggcgcccg gcgagcgcgg accacgccgc
     2221 cacgaagacg gccgctcccg cgctgacgac gaagacgttc gagagcgcag cggcgagctg
     2281 cgagctcgtc aggcgggcga agaagccggc gatcttctcc tcgcggtcgt cgcccttcgt
     2341 ctcgcgcagg atgctcgcga gggcggccgc cgtcatcgca ggctgcttcg tcgcgaggat
     2401 gaggccgaac gcctgcatca cgaggaagct gatcgcgtag ttgaggccgt agaggaagcc
     2461 ttcggggaac gccgcgaggt gccactcgtg gacgagcatc ttgaacgccg cggtgaagac
     2521 ggtgacgatc ccgccgccgg cggccgcgag ccagatgtgt cggtactccg ggcgggacgc
     2581 ggcgacgtag tgctccccgg tctttccggt ccggtcgacg atcttccggt cgaggaggtg
     2641 gaggttccac ctcacgaggt ggaggacgct ccggtcttcg tgggcgtgcc tcacgaggcg
     2701 ggaaaggagc ctgtggaggg cccgcgagcg gtcggggccg ggagggagcg tcatcacgtc
     2761 ggccatgagg gccgttcgtg tcaggcagcg gtcgatcacc tcgaggccga agacgacgtc
     2821 gacgctgacg cccgcctcct cgaggcgcct ctggatggcc cggacctcct ttcggcactc
     2881 ccccgaatcc ttgttgaagg cctgcaggac cgcgccgacc tcccgttcgc agagccaggc
     2941 ttcgaggagg gcgtctccgg agcggaggag ccgatggaac ggggacgcgg agacggagcc
     3001 gggcgtcgcg cgcgagcgca gctcccgcga cagcccctgc gcgtggattc gcgccagcag
     3061 gagccggaag ccgtcggcga aggccctcga aagggaagcg gccgcctcgc gggtgagccc
     3121 cgtctcgaag aggcggacga gccggtggag gtcggagagg gggaggtcgc cgaaggcgtc
     3181 gacgtcggcc tcggtccgga agagccgggc ggcgatctcg acgaagtcat gggcgtcgcg
     3241 gggagacggg atcagccgtg cggagacgcg gcccccgagc tctgcgaaga agcccttgtc
     3301 gctcggaatg ccgacgtcgc cgaagagcgc ggtgccgtcg ccctcggcga agagcgccga
     3361 gagggccgag aggaacccgg cccggaggtc gggggaggct tcgagcgcgt cgaggatcgc
     3421 gccgatccgg gagcgcgggc cgttcgcgcc cggccggggg gcgggcgagt ctcgaaggag
     3481 ccggacgaag gcgtcggcgc gctcctcggg cgagcttgcg gagaggaccg tctcgacgag
     3541 cgcgctcacg acgagcggaa ggatagcgtc tcccgattgc ccggtccgga agcataattt
     3601 cgtcgtgccc gcgaacctga cgccgcctta ccacgacgcc gaggagcggt tcaaggcggc
     3661 gaccacgcac gaggagaggc tcgcggcgat gcgcgagatg atcgcgctcc tcccgaagca
     3721 caaggggacc gagaagctct acgcggacct caagaagagg ctcgcgaagc tcgagagcga
     3781 ggtcgagcac gccgcgcacg gcgggcggcg ggcggacccg gggcacgtga agcgcgaggg
     3841 ggccggccag tgggtcctcc tcggcgagcc gaacgcgggg aagtcctctc tcctcgccgc
     3901 gctcacccac gcccacccgg agattgggga gtacccgttc acgacccgcg tcccgctccc
     3961 cggcatgatg ccgttcgaag acgttcaggt gcagctcgtc gacaccccgg cggtcgccgc
     4021 gggtcatacc gagccctacc tgacgaacct cgcccacaac gccgacggcg tcctgctcgt
     4081 cctcgacgtg gccgcggacg acggggagga gtcggcgaag ctcctcctcg agctcctcga
     4141 gaaggcgcgc gtctggccgc gcgtgaggcc cctcccgccc gacgcgccgc cgttcctcgc
     4201 cgtgaggccc gtgattgcgc tcgggaacaa gcgtgacctc gacgacggga ccttcgccga
     4261 gatggcgcgc gaggcgatcg gggcggacct gcccttccac ccggtctcgg ccctctcggg
     4321 cgaagggctc gacgacctgc ggctcctcct ctggcgcgag ctgcggcgga tccgcgtcta
     4381 cgcgaaggag cccgggaaga agcccgacct cgccgccccg ttcgtcctcc ccgacggcgc
     4441 gacggtccat gacctcgcgg agcgggtcca taaggacgtc gccgccgcgc tgaagttcgc
     4501 gcggatctgg ggtcacgcga agttcgacgg gcagcaggtc gaccgggccc acgtcctcgc
     4561 ggaccgcgac gtcgtcgagc t
//
LOCUS       DAOTEZ010000357         4573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_13752_length_4573_cov_6.842629, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000357 DAOTEZ010000000
VERSION     DAOTEZ010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13752_length_4573_cov_6.842629"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..659)
                     /locus_tag="P5164_15720"
     CDS             complement(102..659)
                     /locus_tag="P5164_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45385.1"
                     /translation="MKPIAEVLSLPLAILQPRATARSFELRAGEELAGTLAFRSSFGS
                     LALARTAESAWTFKRVGSFSPRATVRNEGSERDLVVYTPRWSGQSGEIALEGGERLRF
                     AATGFWGTRFTLSDAAGQTLATFGPEPEAHRFSDLFRAQAVVAVDSAASARPDLGLLL
                     LFGWYLVVLHHEDATAATVAVTAAG"
     gene            complement(727..993)
                     /locus_tag="P5164_15725"
     CDS             complement(727..993)
                     /locus_tag="P5164_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45386.1"
                     /translation="MSADRTASGIRSSLPPRRRFRFSLPFLRRADGGPTPPRPPRRIP
                     CARPGCPNLVVDEWEESGLLCAECALEEDLCDREGRWDRMYPGR"
     gene            complement(1004..>1228)
                     /locus_tag="P5164_15730"
     CDS             complement(1004..>1228)
                     /locus_tag="P5164_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006236558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRY45387.1"
                     /translation="LQRSWIDLLAGVVAAAIREGQLRPDSDPDQLAHDAYGAMLAYHH
                     ASRLLDDPAAEERARRSFASLLDARRTPAS"
     assembly_gap    1229..1328
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1329..1435)
                     /locus_tag="P5164_15735"
     CDS             complement(<1329..1435)
                     /locus_tag="P5164_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015345952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRY45388.1"
                     /translation="MGKGESTREAILRHAIVVASRVGLSGLTIGRLAQEL"
     gene            1585..2325
                     /locus_tag="P5164_15740"
     CDS             1585..2325
                     /locus_tag="P5164_15740"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRY45389.1"
                     /translation="MFRNRTSAVPALVLTVLLAGCQKDAGANPPAAAPAAAVEPKTDD
                     EKTLYAMGIVMGRNLTALGLSAAELDMLKVGMTDEALGKEKKVKLEEFGPKIQAFAQG
                     RAGALAAKEKQASESFLATAAAAKGAQKKPSGLIYTEVLAGTGASPTATDTVKVHYTG
                     KLVDGTVFDSSVERGQPAEFRVGGVIPCWQEGVQLMKKGGKAQLVCPSDIAYGDAGSP
                     PQIKPGATLVFDVELLDIVKGPAAPGAK"
     gene            2403..2714
                     /locus_tag="P5164_15745"
     CDS             2403..2714
                     /locus_tag="P5164_15745"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRY45390.1"
                     /translation="MAEIEFHRSPAPDMPHTRPPATLLDLRKGEEAILDRIDLPDDVS
                     LRLMELGFLPGSRVAASFRAPGGDPRVFRVDGTEVALRCETAARLHLRPLPKPGAASA
                     T"
     gene            2711..>4573
                     /gene="feoB"
                     /locus_tag="P5164_15750"
     CDS             2711..>4573
                     /gene="feoB"
                     /locus_tag="P5164_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HRY45391.1"
                     /translation="MNAASTLPVRPAGEAAPPPSRVVAVVGPPNSGKSTLFNRLTGLR
                     QKVANFPGVTVEQHLGKLRIEDEAELDVIDLPGVYSLNPRSEDERVTLDVLMGRMEGV
                     PRPDTILLILDVTNLGRHLVLAAPVLALGLPTLIVLNMADELERRGGDVQVEELARRL
                     GAPVALISAASGEGYDTILQFLRGRIGIPRPVELPALNDLPRCREWAARLGAEAKYLP
                     PAPPVWTRRLDSLFLHPVAGPLVFLAVVVGVFQTIFSAAAPLMDLVDRAVGASGDLVG
                     RLLPDGPLRSLVVEGIWGGVGSVLVFLPQILILFLFIGLLEDSGYLARAALIADRTMA
                     KVGLQGKSFIPLLSAYACAVPAIMATRTIENKRDRIATILIAPFMTCSARLPVYTLII
                     AAFLPETPLLGPFLGTRAAALLGLYVLGFAAAVVTARVLKSSILKSERTPFVLEMPPY
                     RRPTLRQVGLRLFDRSKVFLRRAGTVILAMSILLWGLAHVPTKNGKAPEIADSFAGTI
                     GRTVEPLIEPLGFNWKIGIGLITSLAAREVIVGTLGTIYGIEGEDDGAGLQAALRHDL
                     TPAGAVALLVFFAFAMQCMSTIAVVRRETGGWTWPILQFTYMGVLAWVGAFVAFR"
BASE COUNT          625 a         1670 c         1555 g          623 t
ORIGIN      
        1 tactgcttgg agactttcga gaaggagatc atgcgggcgg cggacgatag caggccgctg
       61 gcccggaggg gccggcgcct ctgcgatcga cgcggggggc ctcagccggc ggcggtgacg
      121 gcgaccgtgg cggcggtggc gtcctcgtgg tggaggacga cgaggtacca gccgaagagg
      181 aggaggaggc cgagatcggg gcgggccgag gccgcggagt cgacggcgac gacggcctgg
      241 gcgcggaaga ggtcggagaa gcggtgcgcc tccggctcgg gtccgaacgt cgcgagcgtc
      301 tgccccgcgg cgtcggagag ggtgaagcgc gttccccaga aacccgtggc ggcgaagcgg
      361 aggcgctcgc ctccttcgag ggcgatctcc ccggactggc cggaccagcg aggcgtgtag
      421 acgacgaggt cgcgctccga accctcgttc cggacggtcg cccggggcga gaaggagccg
      481 acccgcttga aggtccaggc ggactccgcg gtccgggcca gggcgaggga gccgaaggag
      541 cttcggaacg cgagcgtccc ggcgagctcc tctccggcac ggagctcgaa ggaacgcgcc
      601 gtcgctctcg gctgcaggat tgcgagcggg agcgagagga cctccgcgat cggcttcata
      661 gatacctctc ttcggggcgt gcgaccgcgt cggcggcgcg tgccgccgcc accgtcgccc
      721 gagacactaa cgcccgggat acatccggtc ccagcgcccc tcgcggtcgc agaggtcctc
      781 ctcgagggcg cactcggcgc agagcaggcc cgactcctcc cactcgtcca cgacgaggtt
      841 cgggcagccc ggccgcgcgc acgggatgcg gcgcgggggg cgcggcggcg tcgggcctcc
      901 gtcggcacga cggaggaagg ggagcgagaa ccggaacctc cggcgagggg ggaggctcga
      961 gcggatcccg gaggcggtgc ggtcggcgct catcgagctc ctttcaggag gcgggggtcc
     1021 ttcgggcgtc gaggagagag gcgaaggagc gccgtgcgcg ctcttccgcg gccgggtcgt
     1081 cgaggagccg ggaggcatgg tggtaggcga gcatcgcccc gtaggcgtcg tgggcgagct
     1141 gatcggggtc ggaatcgggg cggagctggc cctcgcggat cgcggcggcg acgacgccgg
     1201 cgaggagatc gatccagctc cgctggagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnag ctcctgcgcc aggcgcccga tcgtgagccc cgagagcccg acccggctcg
     1381 cgacgacgat cgcgtgccgg aggatcgcct cccgggtcga ctctcccttt cccatcctct
     1441 caaaatagca cgaccgttcg ttcggtgctt cgaacccgcc ctcccacggc ggcccggttg
     1501 cgcgtagcat ccgccgacgc tcgaccgcac ggcctcccga aggcgccgca tcgcggcttc
     1561 cgggcgcagg agaaaggagt cctgatgttc cgtaaccgta cgtcggccgt cccggccctc
     1621 gtcctcaccg tgctcctcgc aggctgccag aaggacgccg gggccaaccc tccggccgcc
     1681 gcccccgccg ccgcggtcga gccgaagacg gacgacgaga agactctcta cgccatgggg
     1741 atcgtcatgg ggcggaacct cacggccctc ggcctctcgg cggccgagct cgacatgctc
     1801 aaggtcggga tgacggacga ggccctcggc aaggagaaga aggtcaagct cgaggagttc
     1861 ggcccgaaga tccaggcctt cgcccagggg cgcgcgggcg cgctcgccgc gaaggagaag
     1921 caggcgtccg agtcgttcct cgcgacggcc gccgccgcca agggggccca gaagaagccg
     1981 tccggcctca tctacaccga ggtcctcgcc ggcaccggcg caagcccgac cgcgaccgac
     2041 accgtgaagg tccactacac cggcaagctc gtcgacggga cggtcttcga cagctccgtc
     2101 gagcgcggcc agccggccga gttccgcgtc ggcggcgtca tcccctgctg gcaggaaggc
     2161 gtccagctga tgaagaaggg cggcaaggcc cagctcgtct gcccgtcgga catcgcctac
     2221 ggcgacgccg gctcgccccc gcagatcaag ccgggcgcga cgctcgtctt cgacgtcgag
     2281 ctcctcgaca tcgtcaaggg ccccgcggcc cccggcgcga agtaagccgt tccgagcggc
     2341 ggggccggat ctccggcccc gccttcgctt gacggaccgg cgcggcgcag gcatcctacc
     2401 cggtggctga gatcgagttt caccgctcgc cggcccccga catgccgcac acccgccccc
     2461 ccgcgaccct cctcgatctc cgaaaggggg aggaagcgat cctcgaccgg atcgacctcc
     2521 ccgacgacgt ctcgctccgg ctcatggagc tcggcttcct ccccggcagc cgcgtcgccg
     2581 cctcgttccg cgctccggga ggcgatcccc gcgtcttccg cgtcgacgga acggaggtcg
     2641 cgcttcgctg cgagaccgcc gcccgcctcc acctccggcc gctcccgaag cctggcgcgg
     2701 cgagcgccac gtgaacgcgg cctccaccct gccggtccgg ccggccgggg aagcagcccc
     2761 gccgccttcg cgcgtcgtcg ccgtcgtcgg cccgccgaat tccggcaagt cgacgctctt
     2821 caacaggctc acgggcctcc ggcagaaggt tgcgaacttc cccggcgtca ccgtcgagca
     2881 gcacctcggc aagctccgga tcgaggacga agccgagctc gacgtcatcg acctcccggg
     2941 cgtctacagc ctgaacccgc gctccgagga cgagcgcgtc accctcgacg tcctgatggg
     3001 gcggatggaa ggggtcccgc gccccgacac gatcctcctg atcctcgacg tcacgaacct
     3061 cgggcgccac ctcgtcctgg cggcgccggt cctcgcgctc ggcttgccga cgctcatcgt
     3121 cctgaacatg gccgacgagc tggagcggcg cggcggagac gtgcaggtcg aggagctcgc
     3181 ccggcgcctc ggcgcgcccg tcgccctcat cagcgcggcg agcggcgagg ggtacgacac
     3241 gatcctccag ttcctgcgcg ggcggatcgg catcccgcgc cccgtcgagc tccccgccct
     3301 gaacgacctg ccgcgctgcc gcgagtgggc cgcgcgcctc ggcgccgagg cgaaatacct
     3361 cccgccggcg ccccccgtct ggacccgccg gctggacagc ctcttcctcc acccggtcgc
     3421 cgggccgctc gtcttcctcg ccgtcgtcgt cggggtcttc cagacgatct tcagcgccgc
     3481 cgcgcccctc atggacctcg tcgaccgcgc cgtcggagcg tcgggcgacc tcgtcggccg
     3541 actgctgccc gacgggccgc tccggagcct cgtcgtcgag gggatctggg gaggcgtcgg
     3601 ctccgtcctc gtcttcctcc cgcagatcct gatcctcttc ctcttcatcg gcctgctgga
     3661 ggactccggc tacctcgcgc gcgcggcgct catcgccgac cggacgatgg cgaaggtggg
     3721 cctgcagggg aagtcgttca tcccgctcct gtcggcctac gcctgcgcgg tgccggcgat
     3781 catggcgacg cgcacgatcg agaacaagcg cgaccggatc gccacgatcc tgatcgcgcc
     3841 gttcatgacc tgctcggcgc gccttccggt ctacacgctc atcatcgcgg cgttcctccc
     3901 cgagacgccg ctcctcggcc ccttcctcgg gacgcgcgcc gccgccctcc tcggcctcta
     3961 cgtcctcggc ttcgccgccg ccgtcgtcac cgcgcgcgtc ctgaagtcgt cgatcctgaa
     4021 gagcgagcgg actcccttcg tcctcgagat gccgccgtac cggcggccca cgctccggca
     4081 ggtcggcctc cgcctcttcg accgctcgaa ggtcttcctg cgacgcgccg ggaccgtcat
     4141 cctcgcgatg tcgatcctcc tctggggcct cgcccacgtc ccgacgaaga acggcaaggc
     4201 gcccgagatc gccgacagct tcgcagggac gatcggccgg acggtcgagc cgctcatcga
     4261 gccgctcggc ttcaactgga agatcggcat cggcctcatc acctccctcg ccgcgagaga
     4321 ggtgatcgtc gggacgctcg ggacgatcta cggcatcgag ggggaggacg acggcgccgg
     4381 cctccaggcc gcgctccgcc acgacctgac gcccgcgggc gccgtcgcgc tcctcgtctt
     4441 cttcgcgttc gcgatgcagt gcatgtcgac gatcgccgtc gtgaggcggg agacgggcgg
     4501 ttggacctgg cccatcctcc agttcaccta catgggcgtc ctcgcctggg tcggggcgtt
     4561 cgtcgcgttc cgc
//
LOCUS       DAOTEZ010000358         4572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_13754_length_4572_cov_6.417312, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000358 DAOTEZ010000000
VERSION     DAOTEZ010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13754_length_4572_cov_6.417312"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..417)
                     /gene="ortA"
                     /locus_tag="P5164_15755"
     CDS             complement(139..417)
                     /gene="ortA"
                     /locus_tag="P5164_15755"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HRY45392.1"
                     /translation="MRVAKGTWVEIESIVLKPEERAPGLPEDTAKTPYVLRLSGFLEE
                     EAEVGQEVAVKSLVGRTHHGVLRAVNPSYDHSFGATVPELLHVGLREG"
     gene            complement(491..1531)
                     /gene="ord"
                     /locus_tag="P5164_15760"
     CDS             complement(491..1531)
                     /gene="ord"
                     /locus_tag="P5164_15760"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HRY45393.1"
                     /translation="MKRFRVVQWGLGAMGSGMARLMLEKEGLELVGGIDMRPDYVGKD
                     LGEVLGAGRKLGVAVTNDPSSVLDRAKVDLVVIATTSWTKEQMPDLKKILSAGINCIS
                     IAEEMSAPEAQSPALAAEIDALAKANGVSILGTGVNPGFVLDLLVVALSGVCHKVERI
                     EASRVNDLSPYGPTVMKTQGVGTTPEQFAAGVADGSIVGHVGFPESIRMISDALGLGV
                     TRIEESREAIVSKVYRETPHVKVQPGMVAGCAHVAVGYANDKPVVKLVHPQQIHPHLE
                     GQGTGDYINIYGKPEIHMSTGPEIAGGIATMGVAVNMIPHVVAATPGLKSMCDLPVPA
                     ALMGESAYRRRV"
     gene            complement(1840..2148)
                     /locus_tag="P5164_15765"
     CDS             complement(1840..2148)
                     /locus_tag="P5164_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45394.1"
                     /translation="MRRSPRNAEKVVAAIRSFGFDVPALSVELLMKPDQVIRMGLPPL
                     RIEVLTSVTGVEFEECWASRNEMLLGGEPVWLPKLEDLKKNKAASGRPKDLEDLRHLP
                     "
     gene            complement(2222..2437)
                     /locus_tag="P5164_15770"
     CDS             complement(2222..2437)
                     /locus_tag="P5164_15770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45395.1"
                     /translation="MKPSRDAMRIDRTAFEVVTLDAQDDDLAYWLARSVDDRLEAIEV
                     QRQVIYGYDPATDRLRRVFEVASLERG"
     gene            complement(2459..3019)
                     /locus_tag="P5164_15775"
     CDS             complement(2459..3019)
                     /locus_tag="P5164_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011530142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF99 family protein"
                     /protein_id="HRY45396.1"
                     /translation="MPVSHVVGFDDAPFPRGARGNVPVVGCVFSGPVFTGVLSGKVRK
                     DGRNATEVLSRLVTESKWAPQLQLVMLQGIALAGFNVVDLAGLCRLTGLPVLVVARRR
                     PDLDAIRDALLSRVPGGARKWALVEAAGPMEACAGVYVQREGLSLDEAAAAIARTALH
                     GRVPEPLRAAHLVAGGAWGGESRGRA"
     gene            3112..>4572
                     /locus_tag="P5164_15780"
     CDS             3112..>4572
                     /locus_tag="P5164_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45397.1"
                     /translation="MKQSRTSALVGCGLVLLGGSAVAPAAAQERTVVPIVLDVASGAS
                     RFTTELSLANPGSSAVTVALRYTASLGGALGSGTVFETIPAGGQVRYGDAIATLRARG
                     LAIPTTGAQGGTLVVESPGAAIVATARTTTPIASPAGRAGLAYGGLGEADAFAGSASV
                     FGLRTNAADRSNVAVFSTSEQEVTLRVTAFDGESGASAVVDAARTLPPFGWHQYDEVL
                     RMAGFASGWVVVERLVGGAGSFSAYGVVNDNGTSDGSFVPPARFAAPGSALVLPVLVE
                     AGAFLSELVLANGGSEDATFSLTYVESLSPESGPGGTVTIDVPAGRQRIFPDALEELR
                     RRGLAIGPKGAGHAGSVRVVVSGVPLDQVFVAARTASQAPGRGQFGVFTPAVPAGAEA
                     AAEAIVYGLGADAGSRTNVAVLHAGAAGSVPVTLRLQAHDGTAGGAAAGGPLDVTLES
                     GEWAQPDGFFAASGVANGYVRVTRLEGSAPWIAYGVV"
BASE COUNT          600 a         1684 c         1609 g          679 t
ORIGIN      
        1 tagccggtct cggccatcat ccggtcgtag tcgaaggcga cgggggagga gacgtaggtg
       61 tcgtagtcga tcccgaggct cgccttcatg acctcgttct tccgggagag gacggcggcg
      121 tagctcgtgc ccttcatctc agccctcccg gagcccgacg tggagcagct ccggcaccgt
      181 cgcgccgaag ctgtggtcgt aggaggggtt cacggcccgg aggacgccgt ggtgcgtccg
      241 gccgacgagg ctcttcaccg cgacctcctg cccgacctcg gcctcctcct cgagaaagcc
      301 ggagaggcgc aggacgtacg gggtcttcgc ggtgtcctcg ggcagaccgg gggcccgctc
      361 ctcgggtttc aggacgatcg actcgatctc gacccaggtc cccttcgcga ctctcatgct
      421 tcccccgccg cgcgcggcgc ggccggagag ggccgggaac ccggccctct ccccatcggc
      481 tcgtggacgg tcagacgcgg cggcggtacg cgctctcgcc catgagggcg gcgggcaccg
      541 gcaggtcgca catgctcttc aggcccggcg tcgccgcgac gacgtgcggg atcatgttca
      601 cggcgacgcc catcgtcgcg atgccgccgg cgatctcggg gccggtcgac atgtggatct
      661 cgggcttgcc gtagatgttg atgtagtcgc ccgtcccctg cccctcgagg tgcgggtgga
      721 tctgctgcgg gtggacgagc ttgacgaccg gcttgtcgtt cgcgtagccg acggcgacgt
      781 gcgcgcagcc ggcgaccatc cccggctgca ccttcacgtg cggcgtctcg cggtagacct
      841 tgctgacgat cgcctcgcgg ctctcctcga tccgcgtcac gccgaggccg agggcgtcgc
      901 tgatcatccg gatgctctcc gggaagccga cgtgcccgac gatcgagccg tcggccacgc
      961 cagcggcgaa ctgctcgggg gtcgttccga cgccctgcgt cttcatgacg gtcgggccgt
     1021 acggtgagag atcgttcacg cggctcgcct cgatccgctc caccttgtgg cagacgcccg
     1081 agagcgcgac gacgaggagg tcgaggacga agccggggtt gacgccggtg ccgaggatcg
     1141 agacgccgtt cgccttcgcg agcgcgtcga tctccgcggc gagggcgggg ctctgcgcct
     1201 cgggggccga catctcctcg gcgatggaga tgcagttgat cccggccgag aggatcttct
     1261 tcagatcggg catctgctcc ttcgtccagg acgtcgtcgc gatgacgacg aggtcgacct
     1321 tcgccctgtc gaggacgctc gacgggtcgt tcgtcacggc gacgccgagc ttccggccgg
     1381 ccccgagtac ctcgccgagg tccttcccga cgtagtcggg cctcatgtcg atgccgccga
     1441 cgagctcgag gccctccttc tcgagcatca ggcgtgccat gccgctgccc atggcgccga
     1501 ggccccactg gaccacacgg aatctcttca ttggaatcct cctgccggac cgcccgttcg
     1561 aaccgcccgg cggggtctcc gcaggcagga acagtggcgc cgtccgagcg tggattctac
     1621 cgtttcgcac gccgttccgg ccgcctcgga ccagctaacg gccgttccgg agcgggactt
     1681 ggatgggccg cacctcgcgc gcccgtctca gaaccgctcg tcctgacgac gcgtcgcaga
     1741 acggcgcgat ccgcccatcc gcggggggga agggacggga ccccggccgg actgcatccc
     1801 gaggccgcgg cgccgcgcac ggcgggcgga ccctacctct cagggcaggt gcctgagatc
     1861 ctcgaggtcc ttgggccggc cgctcgccgc cttgttcttc ttcaggtcct cgagcttcgg
     1921 aagccagacc ggctcgcctc cgaggagcat ctcgttccgc gaggcccagc actcttcgaa
     1981 ctccactccc gtgaccgagg tcaggacctc gatcctgagc ggcggaagcc ccatccggat
     2041 cacctggtcc ggcttcatca ggagctccac cgagagcgcg ggcacgtcga agccgaagct
     2101 ccggatcgcc gcgacgacct tctcggcgtt ccgtgggcta cgcctcaccc agacatcgat
     2161 gtccgccgtc gcccgcggat agccgtaaag gccgaccgca taccctccga cgaggaggta
     2221 ctcagccccg ttcgagcgaa gcgacctcaa aaactcggcg aagtcggtcg gtagccggat
     2281 cgtagccata gatcacctgc cgctggacct cgatcgcctc gagccggtcg tcgacgctcc
     2341 tcgcgagcca gtaggcgaga tcgtcgtcct gggcgtcgag cgtgacgacc tcgaaggccg
     2401 tccggtcgat cctcattgcg tcgcgcgaag gcttcacgcc cgcattatcc cctctccctc
     2461 acgccctccc ccggctctct ccgccccacg ccccgcccgc gacgaggtgc gccgctcgga
     2521 gcggctcggg gacccggccg tggagggccg tccgcgcgat cgccgccgcg gcctcgtcga
     2581 gcgagagccc ctcgcgctgg acatacacgc ccgcgcacgc ctccatcggt cccgcggcct
     2641 cgaccagcgc ccacttgcgc gcgccgccgg ggacgcggga gaggagggcg tcgcggatcg
     2701 cgtcgaggtc gggccgtctc cgcgcgacga cgaggaccgg gagacccgtg agacggcaga
     2761 ggccggcgag gtccacgacg ttgaagccgg cgagcgcgat cccctggagc atcacgagct
     2821 ggagctgcgg cgcccacttc gactccgtca cgagacgcga gaggacctcc gtcgcgttcc
     2881 ggccgtcctt ccggaccttt cccgaaagga cgccggtgaa gaccggcccg gagaagacgc
     2941 agccgacgac cggcacgttt ccgcgtgcgc cccgcgggaa tggggcgtcg tcgaagccga
     3001 cgacgtggga gaccggcacg ccccgattct ggcggatcgg accgccgcgc gagctcctcc
     3061 ggggctaagc ttccgaggtc ctctctcccc ggaccggccc cggaggtcgt gatgaagcag
     3121 tcgcgtacgt ccgcgctcgt gggctgcggg ctcgtcctcc tcggggggag cgccgtcgcg
     3181 ccggccgccg cccaggaaag gacggtcgtc ccgatcgtcc tcgacgtcgc ctccggcgcg
     3241 tcgcgcttca cgacggagct ctccctcgcg aaccccggga gctccgccgt gaccgtcgcg
     3301 ctccgctata ccgcctcgct gggcggagcg ctcggctcgg gcaccgtctt cgagacgatc
     3361 ccggccgggg ggcaggtgcg ttacggcgac gcgatcgcga cgctccgcgc gagagggctc
     3421 gcgatcccga cgacgggagc gcagggcggg acgctcgtcg tcgagtcgcc cggcgccgcg
     3481 atcgtcgcga cggcgaggac gaccaccccg atcgcctcgc cagccggccg ggccggactc
     3541 gcctacggcg ggctcggtga ggccgacgcg ttcgcgggga gcgcgtcggt cttcggcctt
     3601 cggacgaacg ccgccgaccg ctcgaacgtc gccgtcttct cgacgagcga gcaggaggtg
     3661 acgctccggg tcaccgcgtt cgacggcgag agcggcgcga gcgccgtcgt cgacgcggcg
     3721 cggacgctcc cgcctttcgg ctggcaccag tacgacgagg tcctccggat ggccggcttc
     3781 gcgagcgggt gggtcgtcgt cgaacgactc gtgggggggg ccggctcctt ctcggcctac
     3841 ggcgtcgtga acgacaacgg cacgagcgac ggctccttcg tcccgcccgc ccggttcgcc
     3901 gccccggggt cggccctcgt cctgccggtc ctcgtcgagg cgggcgcgtt cctctcggag
     3961 ctcgtcctcg cgaacggagg gagcgaggac gccacgttct cgctcaccta cgtcgaatcc
     4021 ctctcgccgg agtcgggccc gggcgggacc gtgacgatcg acgtcccggc gggccggcag
     4081 cggatcttcc ccgacgcgct cgaggagctg cggcggcgcg gcctcgcgat cggcccgaag
     4141 ggcgccgggc acgcggggtc ggtacgcgtc gtggtctcgg gagtcccgct cgaccaggtc
     4201 ttcgtcgccg cccggacggc ctcgcaggcg cccgggcgcg gtcagttcgg ggtcttcacg
     4261 ccggccgtcc ccgcaggcgc cgaggcggcc gcggaagcga tcgtctacgg cctcggcgcg
     4321 gacgccggca gccggacgaa cgtcgccgtc ctccacgcgg gcgccgcggg ctcggtcccg
     4381 gtcacgctcc gcctccaggc gcacgacggc accgcgggcg gcgcggccgc cggtggcccg
     4441 ctcgacgtga cgctcgagag cggcgagtgg gcgcagccgg acggcttctt cgcagcgtcc
     4501 ggcgtcgcga acggctacgt ccgcgtgacg cgcctcgagg ggagcgcgcc gtggatcgcc
     4561 tacggcgtcg tg
//
LOCUS       DAOTEZ010000359         4560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_13805_length_4560_cov_5.893452, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000359 DAOTEZ010000000
VERSION     DAOTEZ010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13805_length_4560_cov_5.893452"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1469
                     /locus_tag="P5164_15785"
     CDS             <1..1469
                     /locus_tag="P5164_15785"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRY45398.1"
                     /translation="AGRQASSGTTERIARLSLHSASLDDAALTPDGETLVYLARFEKG
                     SDLWKYVPRKEEVKLVAKLNAERGARLRLDPEGKKAIVLADGKLSSIDLDSGKATPIK
                     ASAKVELKAAEERAALFEHAWRQTLKKFYVTDLHGVDWAAMKTAYARFLPHVVDNRDF
                     AELISEMQGELNASHTGGRYRPQRRDGDATASLAFFPDPAHTGEGVRVLEVIEGGPLT
                     KAGTRVVPGVVIVSIDGAPIAAGANWYPMLNRKAGEPVRLGLLDPKDGKRWEETVRPI
                     APGEQQGLLYDRWVKSRRAEVERLSGGRLGYAHIRGMNDGAYREVFEEVFGRAVDKDG
                     IVLDTRFNGGGNLVEPLTVFLTGQVYYRAVPRGRQIGVEPGNRWTKPSVVVMNEGNYS
                     DAHCFPTAYKALGIGETVGMQVPGTCTSVWWERLQDKTLTFGIPEVGYLDPSGDLTEN
                     KHLDADHVVDNDPALEVTGRDQQLEKAVEVLMKRVPKK"
     gene            1766..2311
                     /locus_tag="P5164_15790"
     CDS             1766..2311
                     /locus_tag="P5164_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45399.1"
                     /translation="MARFRFLIGAALVFGSLPALAQKSGTAGGKGRILYDLSHDAMEK
                     AEVAFSPRVAWATGDESGPGGKRIHVVLSESEAPLLELSQIWSRPTARMKHCAEKKVP
                     FVAVELNPEGGVELLHQCAAGRFATEMVSTFNGLDSVVVTLEVFSAKQVKGTLRGGDG
                     SCGDGVYCEKTEDYLFDAPLN"
     gene            complement(2490..2801)
                     /locus_tag="P5164_15795"
     CDS             complement(2490..2801)
                     /locus_tag="P5164_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45400.1"
                     /translation="METTGLPGAPLIEEGLADLARGLETVPSLLASIGSPRLRLLGIE
                     VPAPLPDAERRLYRLLERDGSGSAHGRYNALVRTLVSFERAAALRRRAASSTRHDCAP
                     S"
     gene            complement(2848..4104)
                     /locus_tag="P5164_15800"
     CDS             complement(2848..4104)
                     /locus_tag="P5164_15800"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRY45401.1"
                     /translation="MRTRALRPALSVAIAFLLLAGCTSSLRLRYRPTLTSPGADPLAA
                     FTIASGNEPVAGNAARLLENGDEIFPAMLEAIASAQSSIHFESYIYRDGKIAREMAAA
                     LAERARAGVSVRLLLDAIGSAGFGEENERLLEEAGASVVFFRPLSVKTLRKVHLRTHR
                     KVLVVDGRIGFVGGVCIADEWMGDADGPGRWRETVVRVEGPVARQLQAAFGRAWLEAT
                     DELLAGRALYPANGHAGEAVCQVMDSTPGFDSNPARLSFLVAVGSATKSIDLTSAYFV
                     LDRTARRALIDAVKRGVRVRLLLQGPHTDHVIVRYAGRNDYSRLLEAGVEVWEYQKAK
                     LHAKTLVIDGLWGSVGSTNVTARSFTWNYESNVHVFDREFARSMVEMFEHDLSSSHRI
                     TLEEWKDRGLGYRILEWFYAILGSQY"
     gene            4185..>4560
                     /locus_tag="P5164_15805"
     CDS             4185..>4560
                     /locus_tag="P5164_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRY45402.1"
                     /translation="MTPSPVYLDYNATTPVDPEVAEAMLPFLTQRFGNPSSGHPYGVE
                     ARAAVEAARRQVASLLGARPEEIVFTSGGTEGSNTVLQGVARLLRGMGRHVVTSAIEH
                     PAVLEPCALLEEDGWAVTRVGVD"
BASE COUNT          703 a         1649 c         1598 g          610 t
ORIGIN      
        1 tggcgggccg ccaggcgtcc tccggaacga cggagcggat cgcccgcctc tccctccact
       61 ccgcgagcct cgacgacgcg gcgctcacgc ccgacggcga gacgctcgtc tacctcgccc
      121 gcttcgagaa gggctccgac ctctggaagt acgtcccgag gaaggaggag gtcaagctcg
      181 tcgccaagct gaacgcggag cggggagccc gcctccgcct cgaccccgag gggaagaagg
      241 cgatcgtcct cgccgacggg aagctctcct cgatcgacct cgactcgggc aaggcgaccc
      301 cgatcaaggc ctccgcgaag gtcgagctga aggccgccga ggagcgggcc gccctcttcg
      361 agcacgcctg gcgccagacg ctgaagaagt tctacgtcac cgacctccac ggcgtcgact
      421 gggcggcgat gaagacggcc tacgcccgct tcctccccca cgtcgtcgac aaccgcgact
      481 tcgccgagct gatctccgag atgcagggcg agctgaacgc ctctcacacc ggcggccgct
      541 atcggccgca gcgccgcgac ggcgacgcca ccgcctctct cgcgttcttc cccgatccgg
      601 cccacaccgg cgagggcgtc cgggtcctgg aagtgatcga gggcggcccg ctcacgaagg
      661 cggggacgag agtcgtcccg ggcgtcgtga tcgtctccat cgacggcgcc ccgatcgccg
      721 ccggcgcgaa ctggtacccg atgctcaacc ggaaggcggg cgagccggtc cgcctcggcc
      781 tcctcgaccc gaaggacggg aagaggtggg aagagacggt ccggccgatc gcccccggcg
      841 agcagcaggg gcttctctac gaccgctggg tcaagtcgcg ccgcgccgag gtcgaacgcc
      901 tctccggcgg ccggctcggc tacgcccaca tccgcggcat gaacgacggc gcctaccgcg
      961 aggtcttcga ggaggtcttc gggcgcgccg tcgacaagga cggcatcgtc ctcgacaccc
     1021 ggttcaacgg cggcggcaac ctcgtcgagc cgctgacggt cttcctcacc gggcaggtct
     1081 actaccgcgc cgtgccgcgc ggccggcaga tcggcgtcga gcccggcaac cgctggacca
     1141 agccctccgt cgtcgtcatg aacgaaggca actactccga cgcccactgc ttcccgacgg
     1201 cctacaaggc cctcggcatc ggcgagaccg tcggcatgca ggtccccggc acctgcacct
     1261 ccgtctggtg ggagcgcctc caggacaaga cgctgacgtt cgggatcccc gaggtcggct
     1321 acctcgaccc ctcgggcgac ctgaccgaga acaagcacct cgacgccgac cacgtcgtcg
     1381 acaacgaccc cgccctcgag gtcacgggcc gcgaccagca gctcgagaag gccgtcgagg
     1441 tgctgatgaa gagggtgccg aagaagtagc gcccgaggat cctctgccgg ccgcgctcgg
     1501 ccgaaggacg ccccgccgcg ggagaccgcg gcggggcgtc ggcgcgagcg gcgccggacg
     1561 aatccggccc cggaccgccc gccggaccgc cggggcgacg gccaccggag cgacgcgggt
     1621 gagcgcggga tcaggtccgc ttcccgtgcg cccggaaggc gctctcggag gccgggcccg
     1681 ccccccttcg cgtacctggc gaccgcgggc tcctcgtgtc gtggcatcct cgcggcacac
     1741 aaactgtaga tcgtgcggag gaaccatggc tcgattccgt ttcctcatcg gcgccgccct
     1801 cgtcttcggg tcgcttccgg ccctggctca gaagagcgga acggcaggcg ggaagggacg
     1861 gatcctctac gacctcagcc acgacgccat ggagaaggcc gaggtcgcct tctcgccgcg
     1921 tgtcgcttgg gcgaccggcg acgagagcgg gccgggcggc aagcggatcc acgtcgtcct
     1981 gtcggagagt gaagctcccc tgcttgagct gtcgcagatc tggagccgcc cgaccgcgcg
     2041 aatgaagcac tgcgcggaga agaaagtccc gttcgtcgcc gtcgagctga accccgaggg
     2101 gggcgtggag ctcctccacc agtgcgcggc cgggcgcttc gccacggaga tggtcagcac
     2161 gttcaacggc ctcgacagcg tcgtcgtcac gctcgaggtc ttcagcgcga aacaggtcaa
     2221 gggaacgctt cgcggcggcg acggctcgtg cggcgacggc gtctattgcg agaagacgga
     2281 ggactacctc ttcgacgcgc cgctgaactg agacgcgagc cgccgcgtct cggtcctcgg
     2341 ggccgcttcc gtcgtccgcg gcagcggggg gcggaacggg ccccctcctc gctcggcgac
     2401 cgtgctggac ctccacccgt tccggacggc cgtcgccccg ccggtcaggt aaacggtcgt
     2461 ttcgccgcgt gcggcttcgc ccagcgcctt caggaaggcg cgcagtcgtg ccgcgtcgac
     2521 gatgccgcac gccgcctcag ggccgccgca cgctcgaagc tgacgagcgt ccggacgagc
     2581 gcgttgtagc ggccgtgagc cgagccggag ccgtctctct cgagaagcct gtacagtctt
     2641 cgctcggcgt ccggcagcgg ggccgggacc tcgattccga ggaggcgcag gcgcggcgag
     2701 ccgatcgagg cgaggagcga cggcacggtc tcgagcccgc gagccaggtc tgcgagtccc
     2761 tcctcgatca gcggcgcccc gggaagtccc gtcgtctcca cgcgcagatc ttgttgaagg
     2821 ccgagcggct cgcgcgcccg ccgcgggtca gtactgcgag ccgaggatcg cgtagaacca
     2881 ctcgaggatc cggtagccga ggccgcggtc cttccactcc tcgagcgtga tccggtgcga
     2941 gctcgaaagg tcgtgctcga acatctcgac catcgaccgc gcgaactccc ggtcgaagac
     3001 gtggacgttc gactcgtagt tccaggtgaa ggagcgggcc gtcacgttcg tcgagccgac
     3061 gctcccccag aggccgtcga tgacgagcgt cttggcgtgg agcttcgcct tctggtactc
     3121 ccagacctcg acccccgcct cgaggagacg gctgtagtcg ttcctcccgg cgtatcggac
     3181 gatcacgtgg tcggtgtgcg gcccctgcag gaggagccgg acgcgaacgc cgcgcttcac
     3241 cgcgtcgatc agggcgcggc gggcggtccg atcgaggacg aagtaggcgc tcgtcaggtc
     3301 gatgctcttc gtcgcggagc cgaccgcgac gaggaacgag aggcgggccg ggttcgagtc
     3361 gaagcccgga gtcgagtcca tcacctggca gacggcctcg cccgcgtggc cgttcgccgg
     3421 gtagagcgcc cgcccggcga ggagctcgtc cgtcgcctcc agccaggcgc gcccgaacgc
     3481 cgcctggagc tgccgcgcca cgggcccttc cacgcggacg acggtctccc gccaccggcc
     3541 gggcccgtcg gcgtcgccca tccactcgtc ggcgatgcag acgccgccga cgaagccgat
     3601 ccgcccgtcg acgacgagga ccttgcggtg ggtccggagg tgcaccttcc ggagcgtctt
     3661 caccgagagc gggcggaaga agacgaccga ggctccggcc tcctcgagga gccgctcatt
     3721 ctcctcgccg aacccggccg agccgatcgc gtcgaggagg agccggaccg agacaccggc
     3781 ccgcgcacgc tccgcgaggg ccgccgccat ctcgcgcgcg atcttcccgt cgcggtagat
     3841 gtacgactcg aagtggatgc tcgactgggc cgaggcgatc gcctcgagca tcgccgggaa
     3901 gatctcgtcg ccgttctcga ggaggcgggc cgcgttcccc gcgaccggct cgttgccgga
     3961 ggcgatcgtg aacgcggcga gggggtccgc tccgggggag gtgagcgtcg ggcggtagcg
     4021 gagacgcagc gagctcgtgc agcccgcgag gagcaggaag gcgattgcga cggaaagggc
     4081 cggtcggaga gctcgggtcc gcactcgatc cacgaggata cgcgctccgg cgctcgcgcg
     4141 ccggaagaca cctccgccgc ccgccccgtg ccgataatca gggcatgacg ccgtctcccg
     4201 tctacctcga ctacaacgcc acgacgcccg tcgacccgga ggtcgccgag gcgatgctcc
     4261 ccttcctcac gcagcggttc gggaacccct cgagcggcca cccttacggc gtcgaggcgc
     4321 gggcggccgt cgaggcggcg cggcggcagg tcgcttcgct actcggggca cggcccgagg
     4381 agatcgtttt cacgagcggc gggaccgaag gaagcaacac ggtgctccag ggcgtcgcgc
     4441 gcctcctccg cgggatgggg cgacacgtcg tcacgtcggc gatcgagcac ccggcggtcc
     4501 tcgagccgtg cgccctcctc gaggaggacg gctgggccgt cacgcgcgtc ggggtcgacg
//
LOCUS       DAOTEZ010000360         4557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_13815_length_4557_cov_6.882719, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000360 DAOTEZ010000000
VERSION     DAOTEZ010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13815_length_4557_cov_6.882719"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="P5164_15810"
     CDS             <1..966
                     /locus_tag="P5164_15810"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HRY45403.1"
                     /translation="PRLLVYRQTWRGQKQDGLVAACSVEDYDADVIKKHEKTRKDKED
                     DRTKHLVVQAAHAEPVFLTYRASDAVDARVAKVTSAPPEIDFEAVDGVRHSLWLVPEE
                     DAAFFVDAFDAIPVLYVADGHHRSKSASRACAALVEKEPIPADHPVRWFPATIFPDRE
                     LRILPYNRLVKDLLGRTPEAFLAEVKKAFHASPADSAEPERKGEVRMYLDGRWYTLTP
                     KEPVPADDPIRSLDVSILQDRLLAPLLGIADPRTDKRIDFVGGIRGTGELEKRVASGE
                     YAAAFSMFPTTIAELMAIADAGETMPPKSTWFEPKLRSGLFVHRF"
     gene            1016..2221
                     /locus_tag="P5164_15815"
     CDS             1016..2221
                     /locus_tag="P5164_15815"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRY45404.1"
                     /translation="MRVLVADKFEETGLEGLRSLGCEVYYEPKLEGEALGWRLRDTAA
                     EVLVVRSTKVTAAEMDQGRNLSLVIRAGAGVNTIDLAAASARAISVSNCPGKNAVAVA
                     ELAWGLILALDRRIAEQTADLKAGLWNKAEYGRARGLAGRTLGIAGFGAIGREVAARG
                     RAFDMEVVAWSPSLTAERAAEAEVVRVASLRELASRSDVVSVHLALTKETRGVLGGAF
                     FDAMRPGALFVNTSRGETVNGAALKEAIATKGIRAGLDVYEREPSGGTGAFDDEVGRL
                     PGVVGTHHVGASTEQAQNAIASEVVRIVAAFKASGEVPNAVNLCGRSPARWLLTVRHR
                     DRVGVLAHVFGALREAGINVQGTENVVFDGAEAASARIQLDSEPTRSLLAKVAEGCRD
                     ILSITLNPL"
     gene            complement(2262..2849)
                     /locus_tag="P5164_15820"
     CDS             complement(2262..2849)
                     /locus_tag="P5164_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRY45405.1"
                     /translation="MSALPVPLDSPREALRAALLAHAPADDAEARDRTEMLALVESEP
                     ACFSRATFAPGHFTGSLFVLSPSDGRVLLHHHRRLDRWLQMGGHDEGEQDPAATALRE
                     GREESGLDDLALLSPAILDLDVHPIPAAKGEPAHAHFDVRYAAVTREPTSARADAAES
                     LGLAWLPLAEAAVRMGEPGAHRALAKAARLLGLAL"
     gene            complement(2846..>4557)
                     /locus_tag="P5164_15825"
     CDS             complement(2846..>4557)
                     /locus_tag="P5164_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRY45406.1"
                     /translation="EEAIAFAAERLLAIRAESGPASILHYRSGGSLGMVKCVVDLFFE
                     SFGPVTVKRGDICSGAGDAAQEADFGEEESHDLFDLERSRTILLWGKNPFISNVHLLP
                     ILKEARSRGARIVLVDPVRHRTATLADTFVAPRPGGDLALALGVAALLLETGRVVPEA
                     PSFCEGFESFRSLALTKGIDAWAREAGVPEDAIELLASALADRPCNVQVGWGMGRRTN
                     GSAIVRAIDALGAISGNLGIPGGGVSFYFKRRGAFDTSFLRKSPPRTLSEPLLGREIL
                     EAKEPPVRAVWVTAANPVAMLPDSTTVARALETREFVVVVDSFLTDTARRAHLVLPTT
                     TLAEDDDLVGAYGNHWLGASVPVVAPPPGVRSDLEIVQALAAEIDRRTAGAREPIAPG
                     VAGTAKEWKARILRKVEPLGLTVERLEKESVRNPLAPTVLFEEGRFPTPSGRMRLLTD
                     LPAPPEEEPGYPLWLFSSSTERSQSSQWAGEPPVVLEATCHPDAAPGLPDGADVVVES
                     AVGSLRARLRLDPSQRTDVLIVPKGGHFDSGTCANALVRARTTDAGEGAAYLDCRVRV
                     RPA"
BASE COUNT          642 a         1620 c         1708 g          587 t
ORIGIN      
        1 ccccgcctcc tcgtctaccg ccagacctgg cggggacaga agcaggacgg cctcgtcgcc
       61 gcctgctcgg tcgaggacta cgacgccgac gtcatcaaga agcacgagaa gacgcgcaag
      121 gacaaggagg acgaccggac gaagcacctc gtcgtccagg ccgcgcacgc cgagcccgtc
      181 ttcctcacct accgggcctc ggacgccgtc gacgcccgcg tcgcgaaggt gacctcggcg
      241 ccgccggaga tcgacttcga ggcggtcgac ggcgtgcgcc actcgctctg gctggtcccg
      301 gaggaggacg ccgccttctt cgtcgacgcg ttcgacgcca tcccggtcct ctacgtggcc
      361 gacgggcacc accgctccaa gtccgcctct cgcgcctgcg cggcactcgt ggagaaggag
      421 ccaatcccgg ccgaccaccc ggtccgctgg ttccccgcga cgatcttccc ggaccgcgag
      481 ctccggatcc tcccctacaa ccggctcgtg aaggacctcc tcgggcggac cccggaggcc
      541 ttcctcgccg aggtgaagaa ggcgttccac gcctctcccg ccgactcggc cgagccggag
      601 cggaagggcg aggtccggat gtacctcgac gggcgctggt acaccctgac gccgaaggag
      661 ccggtcccgg ccgacgaccc gatccgctcg ctcgacgtct cgatcctcca ggaccggctc
      721 ctcgcgccgc tcctcgggat cgccgacccg cgcaccgaca agcggatcga cttcgtcggc
      781 gggatccgcg ggacgggcga gctcgagaag cgcgttgctt cgggggagta cgcggcggcg
      841 ttctcgatgt tcccgacgac gatcgccgag ctgatggcga tcgcggatgc gggcgagacg
      901 atgcccccga agtcgacctg gttcgagccg aagctccgct ccgggctctt cgtccaccgg
      961 ttctgagtct cccggggcca cggccccgta cgacgacccg aaggggaggc cgccgatgcg
     1021 cgtattggtc gccgacaagt tcgaggagac ggggctcgaa gggctccgct ccctcggctg
     1081 cgaggtctac tacgagccga agctcgaggg cgaggccctc ggctggcgcc tccgcgacac
     1141 ggccgccgag gtcctcgtcg tccggtcgac gaaggtgacg gcggccgaga tggaccaggg
     1201 ccggaatctc tctctcgtca tccgggccgg cgccggggtc aacacgatcg acctcgcggc
     1261 ggcctccgcg cgggcgatct cggtgtcgaa ctgccccggg aagaacgccg tcgccgtggc
     1321 cgagctcgcc tgggggctca tcctcgcgct cgaccggcgg atcgccgagc agacggccga
     1381 cctgaaggcg ggcctctgga acaaggcgga gtacggcagg gcccgcggcc tcgcgggccg
     1441 gacgctcggg atcgccggct tcggcgcgat cgggcgggag gtcgcggcgc ggggtcgggc
     1501 gttcgacatg gaggtcgtcg cctggagccc ctccctgacg gcggaacggg ccgccgaggc
     1561 ggaggtcgtc cgcgtcgcgt cgctccgcga gctggcctct cgctccgacg tcgtgtccgt
     1621 ccacctcgcg ctcaccaagg agacccgggg cgtcctcggc ggcgccttct tcgacgcgat
     1681 gcgccccggc gccctgttcg tcaacacgag ccgcggcgaa acggtgaacg gcgcggccct
     1741 gaaggaggcg atcgcgacga aggggatccg ggcgggcctc gacgtctacg agcgggagcc
     1801 gtcgggcggg actggggcct tcgacgacga ggtcgggcgc ctgcccggcg tcgtcgggac
     1861 ccaccacgtc ggcgcgtcca cggagcaggc ccagaacgca atcgcgtccg aggtcgtccg
     1921 gatcgtggcc gccttcaagg cgtcgggcga ggtgccgaac gccgtgaacc tctgcgggcg
     1981 ctcgcccgcg cgctggctcc tgacggtgag gcaccgcgac cgggtcggcg tcctcgccca
     2041 cgtcttcggc gcgctgcgcg aggcggggat caacgtgcag gggacggaga acgtcgtctt
     2101 cgacggcgcc gaggccgcga gcgcacggat ccagctcgac agcgagccga cgcggagcct
     2161 cctcgcgaag gtcgccgagg ggtgccggga catcctttcg atcacgctga acccgctctg
     2221 aacgccccgc gccgagcgcg gcccgtcgac cgtaggaaag ctcagagagc gaggccgagg
     2281 agccgggccg ccttcgcgag cgcgcggtgg gcgcccggct ctcccatcct cacggccgcc
     2341 tccgcgagag gcagccaggc gagaccgagc gactcggccg cgtcggcccg ggcggacgtc
     2401 ggctcgcgcg tgaccgccgc gtagcggacg tcgaagtggg cgtgcgccgg ctcccccttc
     2461 gccgcgggga tcgggtggac gtcgaggtcg aggatcgccg gcgagaggag ggcgaggtcg
     2521 tcgagtcccg actcctcccg cccttcccgg agcgcggtgg cggccgggtc ctgctccccc
     2581 tcgtcgtgcc ctcccatctg gagccagcgg tcgaggcgcc ggtgatggtg gaggaggacg
     2641 cgcccgtcgg agggggagag gacgaagagg ctccccgtga agtgtcccgg ggcgaacgtc
     2701 gcgcgtgaga agcaggccgg ctcgctctcg acgagcgcga gcatttccgt gcggtcgcgg
     2761 gcctcggcgt cgtcggccgg cgcgtgcgcg aggagggccg cgcgcagcgc ctcccgcggg
     2821 gagtcgagag gaacgggaag cgcgctcacg cggggcggac cctcacgcgg cagtcgaggt
     2881 aagcggcccc ctcgccggcg tcggtcgtgc gcgcgcggac gagcgcgttc gcgcaggtcc
     2941 cggaatcgaa gtggccgcct ttcggcacga tcaggacgtc cgtccgctgc gaagggtcga
     3001 ggcggaggcg ggcccggagg ctcccgaccg cgctctcgac gacgacgtcg gcgccgtccg
     3061 gcaggccggg cgccgcgtcg gggtgacagg tcgcctcgag gaccaccggc ggttccccgg
     3121 cccactgcga gctctgggag cgctccgtcg agctcgagaa gagccagagc ggatagcccg
     3181 gctcctcttc gggcggcgcg gggagatcgg tcaggaggcg catgcggccg ctcggggtgg
     3241 ggaagcggcc ctcctcgaag aggacggtcg gcgcgagcgg gttgcgcacc gactccttct
     3301 cgagccgctc cacggtcagg ccgagcggct ccactttccg gaggatccgc gccttccact
     3361 ccttcgccgt gccggcgact cccggcgcga tcggctcccg ggccccggcg gtcctgcggt
     3421 cgatctcggc ggcgagcgcc tggacgatct cgaggtcgct tcgcaccccg ggcggaggag
     3481 cgacgaccgg caccgaggcg cccagccagt ggttgccgta ggcgcccacg aggtcgtcgt
     3541 cctccgcgag cgtcgtggtc ggaaggacga ggtgggcccg gcgggcggtg tcggtcagga
     3601 acgagtcgac gacgacgacg aactcgcgcg tctcgagggc gcgagccacg gtcgtcgagt
     3661 cggggagcat cgcgacgggg ttcgcggcgg tgacccagac ggcgcggacc ggcggctcct
     3721 tcgcctccag gatctcccgg ccgaggagcg gctcggagag ggtcctcggc ggagacttcc
     3781 ggaggaacga cgtgtcgaac gcgccgcgcc gcttgaagta gaagctgacg cctccgcccg
     3841 ggatgccgag gttcccgctg atcgcgccga gggcgtcgat cgcccggacg atcgccgagc
     3901 cgttcgtccg ccggcccatg ccccagccga cctggacgtt gcagggacgg tcggcgagcg
     3961 ccgaggcgag gagctcgatc gcgtcctccg ggacgccggc ctcccgggcc caggcatcga
     4021 tccctttcgt gagcgcgaga gagcggaacg actcgaagcc ctcgcagaag gagggggcct
     4081 cggggacgac tcggcccgtc tcgaggagga gcgccgccac gccgagcgcg agcgccaggt
     4141 cgcctcccgg gcggggcgcg acgaaagtgt cggcgagcgt ggccgtcctg tggcggaccg
     4201 ggtcgacgag gacgatccgg gcgccgcgcg acctcgcctc cttcaggatc ggaaggaggt
     4261 ggacgttcga gatgaacggg ttcttccccc agaggaggat cgtccgcgac cgttcgaggt
     4321 cgaagaggtc gtgcgactcc tcctctccga agtcggcctc ctgcgccgcg tcgcccgcgc
     4381 ccgagcagat gtcgccgcgc ttcacggtga ccgggccgaa cgattcgaag aagaggtcga
     4441 cgacgcactt caccatcccg agcgagccgc cggaccggta gtggaggatc gaggccggcc
     4501 cggactcggc gcggatcgcg aggaggcgct cggccgcgaa ggcgatcgcc tcctcga
//
LOCUS       DAOTEZ010000361         4543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_13873_length_4543_cov_4.087567, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000361 DAOTEZ010000000
VERSION     DAOTEZ010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13873_length_4543_cov_4.087567"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="P5164_15830"
     CDS             complement(<1..190)
                     /locus_tag="P5164_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45407.1"
                     /translation="MSHASPGEGGVIKRIIRFSAENKYLVLSLYVIAVMIAVWVMKRT
                     PLDAIPDQSDTQVIIYSKW"
     gene            complement(202..1683)
                     /locus_tag="P5164_15835"
     CDS             complement(202..1683)
                     /locus_tag="P5164_15835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY45408.1"
                     /translation="MNKRTLSVALAAGLALAAGSVTLLTACSKSGSHAAHVERYQCPM
                     HPEIVKDGPGDCPICGMKLVPIEEEKPAAQAAAPSAPGERRVAFYRSPMDPNETSPTP
                     KKDAMGMDFVPVYQDELDGAAGPAGLAPVTIDATKRQLLGLRTVTVERAPFATSIRTV
                     GRVAYDERRVHHVHTRYEAYVEDVTADFTGKFVRKGEVLAKVYSPDLYATQKEYLLAL
                     AAARALSGSRADGVAKGGEDLLAAARQRLLLWDIRPADIDALERRGEPTRTLNVYAPI
                     SGFVTGRMAFHGMKVMPADTLFDIVDLSTVWVLADVYEYELPRLSVGQKATVTLAYWP
                     GRAWSGRVTYVYPAVDEATRTVKVRLELPNPGGTLKPEMFAQVVLEGAPRQALVVPDD
                     VVIDSGERKIVFLVEGEGRLVPREIAVGDQDEGHVEVTSGLSAGDVIARGASFLVDSE
                     SRLKAALAAMAPAPAVKPAGGPEGARAPAAVPAPPLPPAGHAH"
     gene            complement(1694..3001)
                     /locus_tag="P5164_15840"
     CDS             complement(1694..3001)
                     /locus_tag="P5164_15840"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRY45409.1"
                     /translation="MALFSAALLLLPIQLLHAQTSASTEVDRLVAEALEKNPELRAAR
                     DLASAARERIRPAGALPDPMVSLSYENDGTAFSLGEMQMTRLSLMAEQQLPFPGKLRL
                     AEEIATKDAERAATLPDRAVLSLTGAVRRAYAGLLEARENLRLVEEQLSTWRGIEEVS
                     RARYAAGLATQQDVLRAQAERTRLAQLREADRAAEETALSEVRQLLFRPPDAAVPTEA
                     RLVPGELLLVPSTRESIARALEATPELREAALAKERERLGADLARRNLKPDFVASAGY
                     MNRGSLPLMWSASVGVTVPLWAGRKQRPLIAEAERLTSAAAATEASLRRLVEALTEER
                     LVRIRRLAAEARLDAEGLLEQDRLSVEAALASYRTGSVPFVSVLEALGTFFADRRSAV
                     GRLAGLLRARADLDELSLERGSGAMPPAMPSAGPASSASPSKM"
     gene            3677..4351
                     /locus_tag="P5164_15845"
     CDS             3677..4351
                     /locus_tag="P5164_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY45410.1"
                     /translation="MRILVVEDDPKLLESVRKGLKEAGFGADGASDGREGLQRALEDD
                     YDALVLDVMLPGLSGLDVLRELRKRRRATPVLVLSARSAVEDRVRGLDLGADDYLAKP
                     FSFAELLARLRAITRRPAVEPQTVLSAGDLSVDTVRHEARRGGHLLELTPKEFSLLEY
                     LARRKGVVLTRAMILDHVWDLDYDGGSNLVEVYVNYLRKKVDAGHEVKLIHTVRGAGY
                     VLREPS"
     gene            4348..>4543
                     /locus_tag="P5164_15850"
     CDS             4348..>4543
                     /locus_tag="P5164_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45411.1"
                     /translation="MTRRPVSFRTRLALVYSGILATVLGLSGAGLYFVVRAQMIRHHD
                     GELAETAAAVEKVLAQHEDCV"
BASE COUNT          634 a         1560 c         1720 g          629 t
ORIGIN      
        1 cccacttcga gtagatgatg acctgcgtgt cggactggtc cgggatcgcg tcgagcgggg
       61 tccgcttcat gacccagacc gcgatcatca cggcgatgac gtagagagag aggacgaggt
      121 acttgttctc ggccgagaag cggatgatcc gcttgatgac tccgccttct cccgggctcg
      181 cgtgactcat cacggcctcc ttcagtgcgc gtggccggcg gggggcagcg ggggggccgg
      241 gacggctgcc ggagctcggg cgccctccgg gccgcctgcg ggcttcacgg cgggggcggg
      301 ggccatcgcg gcgagagccg ccttcaggcg cgactcggag tcgacgagga agctcgcgcc
      361 gcgcgcgatc acgtcgccgg ccgaaaggcc cgacgtcacc tcgacgtggc cctcgtcctg
      421 gtcgccgacc gcgatctcac gcggcacgag gcggccctct ccctcgacga ggaagacgat
      481 cttgcgctcg ccgctgtcga tcaccacgtc gtcggggacg acgagggcct ggcggggcgc
      541 accctcgagg acgacctgcg cgaacatctc cggcttgagc gtgccgcccg ggttcggcag
      601 ctcgagacgc accttgacgg tgcgcgtcgc ctcgtcgacc gccgggtaga cgtacgtcac
      661 gcgtcccgac caggcgcgcc ccggccagta cgcgagggtg acggtcgcct tctgcccgac
      721 ggagaggcgc ggcagctcgt actcgtaaac gtcggcgagg acccacacgg tggagaggtc
      781 gacgatgtcg aagagcgtgt cggcgggcat gaccttcatc ccgtggaagg ccatgcggcc
      841 cgtcacgaag cccgagatcg gagcgtagac gttgagcgtc cgggtcggct cgccacgcct
      901 ttcgagcgcg tcgatgtcgg ccgggcggat gtcccagagg aggagccgct gccgggcggc
      961 ggcgaggagg tcctccccgc ccttcgccac gccgtcggcg cgcgatccgg acagcgccct
     1021 cgccgccgcg agggcgagga ggtactcctt ctgcgtcgcg tagaggtccg ggctgtagac
     1081 cttcgcgagg acctctccct tccggacgaa cttcccggtg aagtccgccg tgacgtcctc
     1141 gacgtatgcc tcgtagcgcg tgtggacgtg gtggacgcgg cgctcgtcgt acgcgacgcg
     1201 gccgacggtg cggatcgacg tcgcgaacgg ggcgcgctcg accgtcaccg tcctgaggcc
     1261 caggagctgc cgcttcgtgg cgtcgatcgt gacgggtgcg agcccggccg gccccgcggc
     1321 gccgtcgagc tcgtcctggt agaccgggac gaagtccatt cccatcgcgt ccttcttcgg
     1381 cgtcggggag gtctcgttcg ggtccatcgg cgagcggtag aacgcgacgc gccgctcgcc
     1441 gggggcggag ggagccgcgg cctgggcggc cggcttctcc tcctcgatcg gaacgagctt
     1501 catcccgcag atcgggcagt cgcccgggcc gtccttcacg atctccgggt gcatcgggca
     1561 ctgatagcgc tcgacgtggg ccgcatgcga cccgctcttc gagcaggcgg tcagcagggt
     1621 gacggaaccg gcggccaggg cgagcccggc ggcgagggcg acggagagag tccgcttgtt
     1681 catctttcga ttcctacatc tttgagggag aggccgagga ggccgggccg gcggaaggca
     1741 tggccggagg catcgcgccg gagccgcgtt ccagggaaag ctcgtcgagg tcggcccggg
     1801 cccgcaggag gccggcgagc cggcccaccg cagagcgacg gtcggcgaag aaggtgccga
     1861 gagcctcgag gacggagacg aaggggacgg agccggttcg atagctcgcg agcgccgctt
     1921 cgacggagag ccggtcctgc tcgaggaggc cttcggcgtc gagccgggcc tcggcggcga
     1981 gccggcggat gcggaccagg cgctcttccg tgagggcctc gacgaggcgg cgcagggagg
     2041 cctcggtcgc ggcagcggcc gacgtgagcc gttccgcttc ggcgatgagg gggcgctgct
     2101 tccgtcccgc ccagagcggg acggtgacac cgacgctcgc ggaccacatg agggggagac
     2161 tgccccggtt catgtagccg gcggaggcga cgaaatccgg cttcaggttc cggcgcgcga
     2221 ggtcggcgcc cagccgctcc cgctccttcg cgagcgccgc ctctcgaagc tccggcgtcg
     2281 cctcgagcgc gcgcgcgatc gactcccggg tcgacgggac gagcagcagc tcacccggaa
     2341 cgagccgtgc ctccgtcggg acggccgcgt ccggcgggcg gaagaggagc tggcggacct
     2401 cggagagggc cgtctcctcg gccgcgcggt ccgcctcgcg tagctgggcc agccgcgtcc
     2461 gctcggcctg cgcgcgaagg acgtcctgct gcgtagcgag gcccgcggcg tagcgggccc
     2521 ggctcacctc ctcgatcccc ctccaggtcg agagctgctc ctcgacgagg cggaggttct
     2581 ctcgggcctc gaggaggccg gcgtaggcgc ggcggacggc gcccgtgagg gaaaggaccg
     2641 cccggtccgg gagcgtggcg gcccgctcgg cgtccttcgt ggcgatctct tcggcgagcc
     2701 ggagcttccc tgggaacggg agctgctgct cggccatcag ggagagccgc gtcatctgca
     2761 tctcgcccag cgagaaggcc gtgccgtcgt tctcgtacga gagggacacc atcgggtcgg
     2821 ggagcgcgcc cgcgggtcgg attcgctcgc gcgcggcgga cgcgaggtcc cgcgccgcgc
     2881 gcagctcggg gttcttctcg agggcctccg ccacgaggcg gtccacctcg gtggacgccg
     2941 aggtctgggc gtggaggagc tggatgggga ggagcagcag ggcggccgag aagagcgcca
     3001 agcttcggcc acgcggtcgt acgggcattc gtcacctcgt gttcggtccg ggaacgggcg
     3061 ggggatacgg ccgaagggac gtggagcgat cagttccgca ggacgcagtt ccgaaggtgc
     3121 agaggcccat ccttgccggg agacaccggc aggcgggttc ccgttcgcgc gtttccgtgg
     3181 ggggtgcccg aggcgacgga cgtggaagct gccgccgccg gaccggccga tgcgcgttcg
     3241 acgcctccct ccgtcgcgac gagagcggcg cgcagcggcg gagcgggacc gaccgtgcag
     3301 ccgcacccgc cgacgcgggc cagagaagag cccgggtcct cgccctggcc gtgtccctcc
     3361 gtcgtcggga ggcaccctgg gcatccggag gcgggtcgat cggtcccgtc gcagcaggga
     3421 gaactcgccg ggcagaccga ggcgagcccg ggtgcgacga ggaggagcgt gagaactccc
     3481 gccgtcgcga gggcgagcgc cgcccgaagc acgcttcggc ccgggccggc ctccgaccgc
     3541 cctggctgag gcggggaggc gatcggcgag agggggggcg cgctcatgac ttcctcgccg
     3601 ggaaggatgc tgcgcgaaga ttagaggcgg attagccgga cgattccgtg agagtctcgc
     3661 gccgggaggg accgcggtga ggatcctggt cgtggaggac gacccgaagc tgctcgagag
     3721 cgtccgcaag ggactgaagg aggctggttt cggcgccgac ggggcctcgg acgggcgcga
     3781 aggcctccag cgagccctgg aggacgacta cgacgcgctc gtcctcgacg tcatgctccc
     3841 cggactttcc ggtctcgacg tccttcgcga gctccggaag cggcgccggg cgacgcccgt
     3901 cctcgtcctg agcgcccgct cggccgtcga agaccgcgtc cgcggcctcg acctcggcgc
     3961 ggacgactac ctcgccaagc ccttcagctt cgcggagctg ctggcgcgcc tccgggcgat
     4021 cacgcgccgt cccgccgtcg agccgcagac cgtcctctcg gccggcgacc tgtcggtcga
     4081 caccgtccgg cacgaggcgc ggcggggggg gcacctcctc gagctgaccc cgaaggagtt
     4141 ctcgctcctc gagtacctgg cgcggcgaaa gggggtcgtc ctgacgcggg ccatgatcct
     4201 cgaccacgtc tgggacctcg actacgacgg cggctcgaac ctcgtcgagg tctacgtcaa
     4261 ctacctccgg aagaaggtcg acgcgggcca cgaggtgaag ctcatccaca cggtgcgcgg
     4321 ggccggctac gtcctcaggg agccgtcgtg acccggcgcc cggtctcctt ccggacccgg
     4381 ctcgccctcg tctactcggg gatcctcgcc acggtcctcg gcctcagcgg cgccgggctc
     4441 tacttcgtcg tccgcgccca gatgatccgt caccacgacg gggagctcgc cgagaccgcc
     4501 gccgccgtcg agaaggtcct ggcacagcac gaggactgcg tgg
//
LOCUS       DAOTEZ010000362         4540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_13887_length_4540_cov_6.052843, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000362 DAOTEZ010000000
VERSION     DAOTEZ010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13887_length_4540_cov_6.052843"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="P5164_15855"
     CDS             complement(<1..1292)
                     /locus_tag="P5164_15855"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRY45412.1"
                     /translation="MDLPSLPSLPTAGGAPAAAPLPAGPEVELSVTGMHCASCVGRIE
                     SALGKVPGVSLAVVDLLSGRARVRLSDPALPVERLLEAVVSVGYEARPAASEESTGLE
                     ELVDAEQAERAAQLAALTRKAAFAGVVAVPVALLSMAEVTFPGRDVLFLVLTALVLAV
                     PARDILVSGARAAARLAPDMNTLVALGTTAAFLTSVAATLFPGALHAEGEHAPVYYEA
                     AVVVVALVLLGRFLEERAKGKAGEAIRSLARLGARKARLVRDGVDVEVPLERVRAGDL
                     LRVLPGEKVPTDGVLVEGATAVDESLVTGESIPVEKAPGDALLGATVNGNGAVVMKAT
                     RVGKETALAQVVRLVRQAQATKAPVQRLVDRVAAVFVPVVVVVALLSFAGWMLLGPEP
                     RLLRALLAVTSVLLVACPCALGLATPTALVVGTGRGAR"
     gene            complement(1297..1590)
                     /locus_tag="P5164_15860"
     CDS             complement(1297..1590)
                     /locus_tag="P5164_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HRY45413.1"
                     /translation="MVAIAPAGSPPADLVARLAKIEGQVRGVSRMVEEKRWCPDILRQ
                     IGAVEAALSRVKERLLESHLDHCVRRVLDSGSAEEKRAAVAEVLEAVKRTRER"
     gene            complement(1674..3104)
                     /locus_tag="P5164_15865"
     CDS             complement(1674..3104)
                     /locus_tag="P5164_15865"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRY45414.1"
                     /translation="MTRLLRGIAPALAFLLFAPAVRADDPLPVETRAAVAALARKGSA
                     QTRAVEWVRRLTDEVGPRLAGSPGDRAAVAWGQALLRELGLANVRAEEVPVKAWERGV
                     ETAEVVAPFRQRLVVAALGGSVPTPEGGVAAPIARMESLEALEEAVKADPDAVRGKIV
                     FFGRRTVKDPAGPGYGKNVPMRSSGPAKAAPHGALAVIVRSVGTSTSRFAHTGSTNYE
                     VGGPRIPAAAVSNTDADLLERLLAKGPVTVRLVLGCRPLPDTTSANVIGEVPGRERPD
                     EVVVVSGHLDSWDLGTGAVDDGAGVAIAVEAARLVAELPRRPRRTIRVVLFANEESGL
                     AGGKSYAERHVAELPKHAGAFEADSGTGAPQGFSWNAADTLEPALAAVAKLLEPLGAG
                     ALRKGGAGGADISPMLPSGVPLFGLRQDSATYFDVHHTADDTFDKIEPASLDRAAAAV
                     AAMAYAVADLPEIHGRPAEAPAAKPR"
     gene            complement(3128..>4540)
                     /locus_tag="P5164_15870"
     CDS             complement(3128..>4540)
                     /locus_tag="P5164_15870"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRY45415.1"
                     /translation="DGLRDVDEAWFDGVPIGGLGGFPPRPETAHFVARLYPLPTDRVD
                     EAGPRELVLRVWHGRRDGSVFRSPPVIDRVDRLERRRSLRDQGIVLFVVAALVVAGLL
                     VLFAFHARSPADYLLFAGFAVSLGLWVTTGHSRWGELPFPLSTVFRAGLVALSATAVC
                     YGAAMLRFLGGMRPVHRVLLPALGLLAPLAALLPDAEGLVLPVLFFRWAFGALLLDLL
                     VRFGIAAYRRRRLARTDLLGHVAFVVAAIPVAGLLPGAELPRIDLSGRVLLLGFLFLC
                     LASAVLLRMSEEIRRYRLAGLTDPGTRLWNREALYEEIAERGEAVRRGRGAGFGLLLA
                     DLDRFREWNDAKGRVAGDRVLLCAARALIDASRPNDLVARYGGDEFAVVVGDVDRETL
                     PALASRLRSAIGDAIEKESGGALTSASIGTVLFDPARHRTPEDLLREADRALWEAREA
                     SRAATPAVRRTPSGKWALRL"
BASE COUNT          658 a         1683 c         1703 g          496 t
ORIGIN      
        1 atccgcgcgc cccgccccgt cccgacgacg agcgccgtcg gcgtggcgag cccgagggcg
       61 caggggcatg cgacgagaag gaccgacgtg accgcgagga gcgcccggag gagccgcggc
      121 tcggggccga ggagcatcca ccccgcgaag gagaggagcg cgacgacgac gacgacgggg
      181 acgaagaccg ccgcgacgcg gtcgacgagc cgctgcaccg gcgctttcgt cgcctgcgcc
      241 tgccgcacga gcctcacgac ctgcgcgagc gccgtctcct tcccgacgcg cgtcgccttc
      301 atcacgacgg cgccgtttcc gttcacggtc gcgccgagga gcgcgtctcc cggcgccttc
      361 tcgaccggga tcgactcccc ggtcacgagc gactcgtcga cggccgtcgc cccctcgacg
      421 aggacgccgt ccgtcggcac cttctcgccc gggagcactc ggaggaggtc gcccgcgcgg
      481 acccgctcga gcggcacctc gacgtcgacc ccgtcgcgca cgaggcgggc cttccgcgcg
      541 ccgagccgcg cgagcgagcg gatcgcctcg cccgccttcc ccttcgcccg ctcctccagg
      601 aagcggccga ggaggacgag cgccacgacg acgacggccg cctcgtagta gacgggcgcg
      661 tgctcccctt ccgcgtggag cgcgcccggg aagagagtcg ccgccacgga ggtgaggaac
      721 gcggccgtcg tgccgagcgc cacgagggtg ttcatgtcgg gggcgaggcg cgcggccgcg
      781 cgggcgccgg agacgaggat gtcgcgcgcc ggaaccgcga ggacgagcgc cgtgaggacg
      841 aggaagagga cgtcccgccc cgggaacgtc acctcggcca tcgagaggag cgcgaccggc
      901 acagcgacga cgccggcgaa cgcggctttc cgggtcagcg ccgcgagctg agccgcccgc
      961 tccgcctgct cggcgtcgac gagctcctcg agccccgtcg actcctcgct cgccgccggt
     1021 ctcgcttcgt acccgacgga gacgaccgcc tcgaggagcc gctcgacggg gagcgcggga
     1081 tcggaaagcc gcacgcgcgc ccggcccgag agaaggtcga cgacggcgag cgagaccccc
     1141 gggaccttcc cgagggcgga ctcgatccgc ccgacgcagg aggcgcagtg cattcccgtg
     1201 acggagagct cgacctcggg gccggccggg agcggagcgg ccgcgggggc gccgccggcc
     1261 gtcgggaggg agggcaggga cgggaggtcc atcggctcac ctctcccgcg tccgcttcac
     1321 cgcctcgagg acctcggcga cggccgcgcg cttctcctcc gcgctccccg agtcgaggac
     1381 ccggcgaacg cagtggtcga ggtggctctc gaggaggcgc tccttcacgc gcgagagggc
     1441 ggcctccacg gcgccgatct gacgaaggat gtcggggcac cagcgcttct cctcgaccat
     1501 ccgggagacg ccgcggacct ggccttcgat cttcgcgagg cgggcgacga ggtcggcggg
     1561 gggagatccg gcgggggcga ttgctaccat acccccgcag ggtatgcgcc gggccgcccc
     1621 gcgtcaacac gccccttccg cctcgaggcg gaaggggcgg cggggcagtc cggtcacctc
     1681 ggcttcgccg ccggggcctc cgccgggcgc ccgtggatct cgggcaggtc ggctacggcg
     1741 taggccatcg ccgccacggc ggcggccgcc ctgtcgagcg aggcgggctc gatcttgtcg
     1801 aacgtgtcgt ccgcggtgtg gtggacgtcg aagtaggtcg cggagtcctg ccggaggccg
     1861 aagagcggga cgcccgaagg gagcatcggc gagatgtcgg cgcctcccgc gccccccttc
     1921 cggagcgcgc ccgcaccgag cggctcgagg agcttggcca cggcggcgag ggccggctcc
     1981 agcgtgtcgg ccgcgttcca cgagaagccc tggggcgcgc cggtcccgct gtcggcctcg
     2041 aacgcccccg cgtgcttcgg aagctcggcg acgtgccgct cggcgtagct cttcccgccc
     2101 gcaaggccgc tctcctcgtt cgcgaagagg acgacgcgga tcgtcctccg gggcctccgc
     2161 gggagctccg cgacgagccg cgccgcctcg accgcgatgg cgacaccggc gccgtcgtcg
     2221 accgcgccgg tgccgaggtc ccacgagtcg aggtggcccg agacgaccac gacctcgtcc
     2281 ggcctctcgc gccccgggac ctctccgatc acgttcgccg aggtcgtgtc ggggaggggg
     2341 cggcagccga ggacgagcct caccgtcacc ggacccttcg cgaggagccg ctcgagaagg
     2401 tcggcgtcgg tgttcgagac ggccgcggcc ggaatcctcg gcccgcccac ctcgtagttc
     2461 gtcgagccgg tgtgcgcgaa gcggctcgtc gaggtgccga cggagcggac gatcaccgcg
     2521 agagcgccat gcggcgccgc cttcgctggc cccgaggagc gcatcgggac gttcttcccg
     2581 taaccggggc ccgccgggtc cttcaccgtc ctgcggccga agaagacgat cttcccgcgg
     2641 acggcgtcgg ggtccgcctt cacggcctcc tcgagcgcct cgaggctctc catcctcgcg
     2701 atcggggcgg cgacaccgcc ctccggcgtc gggacgctcc cgccgagcgc ggcgacgacg
     2761 aggcgctggc ggaaaggggc gacgacctcg gccgtctcga cgccccgctc ccaggccttc
     2821 accggcacct cctcggcgcg cacgttcgcg agtccaagct cccggaggag cgcctgcccc
     2881 cacgcgaccg ccgcgcgatc gccgggcgag ccggcgaggc gggggccgac ctcgtcggtg
     2941 agccggcgga cccactcgac ggcgcgcgtt tgcgcgctcc ccttccgagc gagagcggcg
     3001 acggccgccc tcgtctcgac gggaagaggg tcgtcggccc ggacggctgg cgcgaagagg
     3061 agaaacgcga gcgccggcgc gatgccacga aggagtctcg tcacgaggac ggagtctacg
     3121 gaaccgctca gagacgcagc gcccacttcc cggacggagt ccggcgcacc gccggggtcg
     3181 ccgcgcgcga tgcctcccgc gcctcccaga gcgcccggtc cgcctcccga aggagatcct
     3241 cgggcgttcg gtggcgcgcc gggtcgaaca gcaccgtccc gatcgacgcg ctcgtgaggg
     3301 ctccgccgct ctccttctcg atcgcgtccc cgatcgcgct ccgcagccgc gaggcgagcg
     3361 ccgggagcgt ctcgcgatcc acgtccccga cgacgaccgc gaactcgtcg ccgccgtagc
     3421 gcgccacgag gtcgttcggt cgcgaggcgt cgatgagagc ccgggccgcg cagaggagga
     3481 cccggtcgcc cgcgacccgc cccttcgcgt cgttccactc ccggaaccgg tcgaggtcgg
     3541 ccaggaggag gccgaagccg gccccccggc cgcggcgaac ggcttctcct cgctcggcga
     3601 tctcctcgta gagcgcctcg cggttccaga ggcgcgtccc cgggtcggtc agccccgcga
     3661 ggcgatatcg ccggatctcc tccgacatgc gcagaaggac ggccgaggcg aggcacagga
     3721 agagaaagcc cagaaggagg acgcgtcccg agaggtcgat ccggggcagc tccgctccgg
     3781 ggagcaggcc ggccaccggg atcgcggcga cgacgaaggc cacgtgcccg aggaggtcgg
     3841 tccgcgcgag ccgtcggcgc cggtacgccg cgattccgaa gcgaacgagg aggtcgagga
     3901 ggagcgcccc gaacgcccag cggaagaaaa ggaccggcag gacgagccct tcggcgtcag
     3961 gcaggagggc cgcgaggggc gcgagcagcc cgagagccgg aagcaggacg cggtggacgg
     4021 gccgcatccc gcccaggaag cgaagcatcg ccgccccgta gcagacggca gtggccgaga
     4081 gggcgacgag ccccgctcgg aagacggtgg agagtgggaa cgggagctct ccccaccggg
     4141 aatgccccgt cgtcacccag agcccgaggg agacggcgaa tccggcgaag agcaggtagt
     4201 ccgcggggct ccgcgcgtga aaggcgaaga ggacgaggag gcccgcgacg acgagcgcgg
     4261 cgacgacgaa gaggacgatt ccctggtccc ggagcgagcg ccgccgctcc aggcggtcga
     4321 cgcggtcgat gacgggcggt gagcggaaga cgctcccgtc gcgccggccg tgccagaccc
     4381 ggaggaccag ctctcggggc cccgcctcgt ccacgcgatc ggtcggcagc gggtagagcc
     4441 gtgcgacgaa gtgcgccgtc tcggggcggg gcgggaagcc gccgagaccg ccgatcggga
     4501 cgccgtcgaa ccacgcctcg tcgacgtcgc ggaggccgtc
//
LOCUS       DAOTEZ010000363         4514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_13994_length_4514_cov_5.549002, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000363 DAOTEZ010000000
VERSION     DAOTEZ010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13994_length_4514_cov_5.549002"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="P5164_15875"
     CDS             complement(<1..492)
                     /locus_tag="P5164_15875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45416.1"
                     /translation="MKCRSARSTSRDRLCRVAFGTALSLAALAPRAGLPAPQQSILPR
                     DAAARPFLEGRALVEKGRFREAVPKLESALSTGHETPQLRFGASRHQVDYYDPHYWLG
                     RALMELGEEESALAHLRASAAAGSFPDRRETEDRRLRIVELERREAARRAPPPRTPTP
                     TPPP"
     gene            546..3668
                     /locus_tag="P5164_15880"
     CDS             546..3668
                     /locus_tag="P5164_15880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45417.1"
                     /translation="MPRNLASARYALLAGLFLVSPAAAGANGSCPPNRPVVSGPSEVH
                     RLEGYWITWTNVLSPRGSGQPDTFLIERSEDPTFQSGVERLQPTTRTTATWGPPGGAP
                     RTIYHRVAVQSFCAGTSPIRVESTVFAVRVTDQCRVPIALAPPVASPALPPANTTYIV
                     SWDTLGAGEPGPGGGAQGLRFRLRRTAGGDFTESLNDAGAAAFTDAPGEYLYQVRTEN
                     ICGDASAWSEPLRVVVGATKTSALFLVSAPRPVLMPADAQGDVRVAFVVRNAGSEPLD
                     VTASGGGGGLAAEPATFRLESGRDRRVEARLAKAPSAEAPFHGAVVLTANGNVSLRVP
                     IDVATRGLDAGGGPSGEPGVSRPAGWDEDAVDVDKNGRDVTRLLVNPGGVTAYLAASV
                     SADWLVVSSADGKAWDRPLAPGESRAIWLRVDRSRRRAAIGTEIATVTVVTAGHEGEP
                     KSLSVIDDGPQVTGVGSPSASDDIPPLYLMKSRLLFPSLPNAMDARGAGRYTSDLWLS
                     NVDALNAIQVVLTLTPAGQSNSSAGVRQFAFSLGAGETRRFRNVIGTIFGFEGGCSLD
                     VSSPSPTLSATALVNNKPVVPLVAGKTAALDSAIAGTVLSTAEFGFEMRPVAPGEGAS
                     QDDPEHVVSGLWHKAERRTNLILRETTGNETTVQVELVDGEGRPVLRDGAAVKLEVKV
                     PAHGSVQVNDADLFPAEEYSEALHAKVTFLRGSIDPFGQTHGGVVPLATVIDAGTQDA
                     ALRVGVSVKSLVPISAETSAQRQALSNALPFGGEPEPLVFPATHVTGASLSSGAPPRW
                     RSRVTLSNVSGTEQRNVRLRYRPANGVLPSGMLEQTSIVVPPGWGITFDNILEQVFGL
                     PAGAQTWGTIEADNLKREDGSWLFTWTDVDVQTETYTADTEVPSRGEFRTGMEAYSYR
                     HGYSSFQSNLGTVLIDGAEISARNRTNLILQEVGGAPCKLAVGVYRPGALVPLAVRII
                     DLKASEYLSQELFRGLMQLDLDEIVDARVVVRQIDGDGVFMAFVSKINLVTGDPANVF
                     LRPASAGTGR"
     gene            3793..>4514
                     /locus_tag="P5164_15885"
     CDS             3793..>4514
                     /locus_tag="P5164_15885"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRY45418.1"
                     /translation="MGIVYRAVDERDGKTVALKVIRETEVAGDESAPHVAEARVRFTR
                     EAEILQGLLHANVVTFHEIGEEDGTPYLAMEFLEGRPIGSYAGRPWTETLPLLVQAAN
                     GMEYLASRGIVHRDLSPDNVFVVESGGEKTVKLLDFGIAKLFESATSESLTATGFFLG
                     KVAYGSPEQLGSLGQGAPLDWRSDVYSLGVIFYQVLAGRRPFEGRAPVEYIAAHLNLT
                     PPPVAAPPDAPALPMELVRLIG"
BASE COUNT          625 a         1644 c         1613 g          632 t
ORIGIN      
        1 ggggggcggc gtgggagtcg gcgtccgcgg cggaggggcc cggcgggcgg cttcgcgccg
       61 ttcgagctcg acgatccgga gcctccggtc ctcggtctcc cggcgatccg gaaaggagcc
      121 ggccgcggcc gaggcgcgca ggtgcgcgag cgcgctctcc tcctccccga gctccatcag
      181 ggcgcgcccg agccagtagt ggggatcgta gtagtcgacc tggtggcggc tcgctccgaa
      241 gcggagctgg ggcgtctcgt ggccggtgga gagcgcggac tcgagcttcg ggaccgcctc
      301 gcggaaccgc cccttctcga cgagagccct cccctcgagg aacggccggg cggccgcgtc
      361 gcgcggtaag atactttgtt gcggtgccgg gaggcccgcc cgcggtgcga gcgccgccag
      421 gctcagggcg gtcccgaagg cgacccggca gagccggtcg cgtgaggtgg agcgggccga
      481 acgacacttc atgcaggcgc gccgcgtcga gtttaccggc ggggcgcgcg aaggggatga
      541 cgacgatgcc gcgaaacctc gcttccgccc gatacgccct gctggcgggc ctcttcctcg
      601 tctcgcccgc agccgcagga gcgaacggat cgtgcccgcc gaaccggccg gtcgtctcgg
      661 gtccctcgga ggtccaccgg ctcgagggct actggatcac ctggacgaac gtcctctcgc
      721 cgcgcggctc gggacagccc gataccttcc tcatcgagcg atcggaggac ccgaccttcc
      781 agagcggcgt cgagcgcctt cagccgacga cccggacgac ggccacgtgg ggccctcccg
      841 ggggcgctcc gcgtacgatc taccaccggg tcgccgtcca gtccttctgc gccgggacgt
      901 cgccgatccg ggtcgaatcg accgtcttcg cggtccgcgt caccgatcag tgccgcgtcc
      961 cgatcgctct cgcgccgccg gtggcgagcc ccgccctgcc gccggcgaac acgacctaca
     1021 tcgtctcctg ggacaccctc ggcgcggggg agccgggtcc gggcggcggg gcgcaggggc
     1081 tccgcttccg cctccggcgg actgccggcg gcgacttcac cgagagcctg aacgacgccg
     1141 gggcggccgc gttcaccgac gcccccgggg agtacctgta ccaggtccgg accgagaaca
     1201 tctgcggcga cgcgagcgcc tggtccgagc cgctgcgcgt cgtggtcggc gccacgaaga
     1261 cctccgccct cttcctcgtc tcggccccgc ggccggtcct gatgcccgcg gacgcgcagg
     1321 gcgacgtacg cgtggcgttc gtggtccgga acgccgggag cgagccgctc gacgtgaccg
     1381 cctcgggagg ggggggcggc ctcgcggccg agcccgcgac gttccggctc gagtccggcc
     1441 gcgaccgccg ggtcgaagcc aggctcgcga aggcgccttc ggcggaggcg ccgttccacg
     1501 gagccgtcgt cctgacggcg aacgggaacg tttcgctgcg cgtgccgatc gacgtcgcca
     1561 cccgaggcct cgacgcgggc gggggcccgt ccggcgaacc cggtgtctcg cgaccggcgg
     1621 gctgggacga ggacgccgtc gacgtcgaca agaacggccg cgacgtcacc cgcctcctcg
     1681 tgaacccggg gggagtcacc gcctacctcg ccgcgtcggt ctccgccgac tggctcgtcg
     1741 tctcctccgc cgacggaaag gcctgggacc ggccgctcgc tcccggcgag tcgcgcgcga
     1801 tctggctgcg ggtcgaccgg agccgccggc gggcggccat cgggacggag atcgcgacgg
     1861 tcaccgtcgt cacggccggc cacgaaggcg agccgaagag cctcagcgtg atcgacgacg
     1921 ggccccaggt gacgggcgtc ggaagcccct cggcgagcga cgacatccct ccgctctacc
     1981 tgatgaagag ccggctcctc ttcccgtccc tcccgaacgc gatggacgcg cggggcgcgg
     2041 ggcgttacac ctcggacctc tggctctcga acgtcgacgc cctgaacgcg atccaggtcg
     2101 tcctgacgct gaccccggcg gggcagtcga actcctccgc cggggtgcgc cagttcgcgt
     2161 tctcgctcgg cgcgggcgag acccgccggt tccggaacgt gatcgggacg atcttcgggt
     2221 tcgaaggggg ctgctctctc gacgtcagct ccccgagccc gaccctctcg gccacggccc
     2281 tcgtgaacaa caagccggtc gtccccctcg tggccgggaa gacggccgcg ctcgacagcg
     2341 ccatcgcggg aacggtcctc tcgaccgccg agttcgggtt cgagatgcgg ccggtcgctc
     2401 ccggcgaagg ggcgagccag gacgaccccg agcacgtcgt ctccgggctc tggcacaagg
     2461 ccgagcgccg cacgaacctg atcctccggg agacgacggg gaacgagacg accgttcagg
     2521 tcgagctcgt cgacggtgag gggcgcccgg tgctgcgcga cggcgccgcc gtcaagctcg
     2581 aggtgaaggt gccggcccac ggctccgtcc aggtcaacga cgccgatctc ttcccggccg
     2641 aggaatactc cgaggcgctc cacgcgaagg tgacgttcct gcgcggctcg atcgacccgt
     2701 tcggccagac gcacggcggc gtcgtcccgc tcgcgaccgt catcgacgcc gggacgcagg
     2761 acgcggcgct ccgggtcggc gtttccgtga agtcgctcgt tccgatctcg gccgagacgt
     2821 cggcccagag gcaggcgctc tcgaacgccc tgcccttcgg aggggagccg gagccgctcg
     2881 tgttcccagc cactcacgtc acgggggcgt cgctctcgag cggagcaccc ccgcggtgga
     2941 ggagccgcgt caccctttcc aacgtctccg ggaccgagca gcgcaacgtc cggctccggt
     3001 accggcccgc caacggcgtc ctgccttcag ggatgctcga gcagacctcg atcgtcgtcc
     3061 cgccgggctg gggcatcacg ttcgacaaca tcctggagca ggtgttcggc ctccccgcgg
     3121 gtgcccagac ctgggggacg atcgaggccg acaacctcaa gcgtgaggac ggctcgtggc
     3181 tcttcacctg gaccgacgtc gacgtccaga ccgagaccta cacggcggac accgaggttc
     3241 cgtcgagagg cgagttccgc accggaatgg aggcgtactc ctaccgccac ggctattcct
     3301 cgttccagtc gaacctcggg acggtcctga tcgacggcgc cgagatctcg gcccgcaatc
     3361 ggacgaacct gatcctccag gaggtcgggg gggcgccctg caagctcgcg gtcggcgtct
     3421 accggcccgg ggcgctcgtg ccgctcgcgg tccggatcat cgacctgaag gcctcggagt
     3481 acctctcgca ggagcttttc cgcggcctga tgcagctcga cctcgacgag atcgtcgacg
     3541 cgcgggtcgt cgtgcggcag atcgacggcg acggggtctt catggcgttc gtctcgaaga
     3601 tcaacctcgt gacgggcgac ccggcgaacg tcttcctccg tccggcctcg gcggggacgg
     3661 gacgctgacc cggggcgggc gggtgcgcgg taacctcggc ggggaagagg gccccgtgga
     3721 ggagacctga acccgcccgc cgtcgagagg atcgcccact accggatcgt ccgccgcgtc
     3781 ggtcgcggcg ggatggggat cgtctaccgc gcggtggacg agcgggacgg gaagaccgtc
     3841 gccctgaagg tgatccggga gaccgaggtg gccggcgacg agtcggcgcc gcacgtcgcc
     3901 gaggcgcgag tccgcttcac gcgggaggcc gagatcctcc agggcctcct ccacgcgaac
     3961 gtcgtcacgt tccacgagat cggcgaggag gacggcacgc cctacctcgc gatggagttc
     4021 ctcgaggggc gtccgatcgg cagctacgcc ggccgcccgt ggacggagac gctcccgctc
     4081 ctggtccagg ccgcgaacgg catggagtac ctcgcgtcgc gcgggatcgt ccatcgcgac
     4141 ctttcgcccg acaacgtctt cgtcgtcgag agcggcgggg aaaagaccgt caagctcctc
     4201 gacttcggga tcgcgaagct cttcgagtcg gccacgtcgg agtcgctcac ggcgaccggg
     4261 ttctttctcg gaaaggtcgc ctacggctcg cccgagcagc tcggctccct cgggcagggc
     4321 gctccgctcg actggcggag cgacgtctac agcctcgggg tcatcttcta ccaggtcctc
     4381 gcggggcggc gcccgttcga agggcgcgcc ccggtcgagt acatcgccgc gcacctgaac
     4441 ctgacgccgc cgcccgtcgc ggccccgccc gacgctcctg ctctcccaat ggagctcgtg
     4501 aggctgatcg gcca
//
LOCUS       DAOTEZ010000364         4503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_14044_length_4503_cov_5.899056, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000364 DAOTEZ010000000
VERSION     DAOTEZ010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14044_length_4503_cov_5.899056"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /gene="prfB"
                     /locus_tag="P5164_15890"
     CDS             complement(<1..913)
                     /gene="prfB"
                     /locus_tag="P5164_15890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRY45419.1"
                     /translation="MFDVHRAEKEKAELEARAGAPDFWNDAPAAQETLKKLKRADTVL
                     SDDTAFRQKREELEILAEFLREGEAVEADADTALSSIASWARERELRFKLTEPEDELP
                     ALLEINAGTGGTEAQDWAEMLMRMYLRYGERKGWKTDVLEVSYADEAGIKSATIRFEG
                     EYAYGHMKAEHGVHRLVRISPFDAAARRHTSFASVYVTPEVDDTIEIEILDKDLRIDT
                     FRAGGKGGQHVNKTDSAVRLTHLPTGLVVACQNERSQHKNKDFAMKVLKSRLYQRAVE
                     EKRAVEEKRAGVKMEIGFGSQIRSYVLA"
     gene            complement(997..2556)
                     /gene="lnt"
                     /locus_tag="P5164_15895"
     CDS             complement(997..2556)
                     /gene="lnt"
                     /locus_tag="P5164_15895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRY45420.1"
                     /translation="MGFPFGPFRARAAAALVSGLLLGLTFPNASLVLLLPVAIVPFGL
                     ALHGASPGRGALLGGLFGVAFWLTTFPWIFHVVHRFGGLSAPLASLALLIAACIPSVA
                     FAAMGWAAALAAPRTAAARLLVLPAAWAAQELLRTYAFSGFPWALVSYPLAPWPVLTQ
                     TAALGGAALVSFLVVLVNAALAEAIRAKGRARLASATVAAAVLLGATGFGAWRLSRPS
                     PEARDRALRVLIVQPNVAQDVRYAPGAAERIHFDVTGLTRALLRKAPADLVLWPESAT
                     PFAWPWSDGLREDLMRLCREERTALLFNTVWSDAPLDAAAPYFNSALLVDGNGPVGEP
                     YHKLRLVPFGEYVPAPSLFGWVNQVSREAPGAFTPGTRAIPLERGGVRLGGAICYEVV
                     YPWITRAQVAAGADALFTLTNDAWYGDGGAQEQHWQAAVFRAVETGRPMLRAAVTGIS
                     GWVDARGRTLARLGPGARAGIAGTLRVPPRGETTLATRLGDAPAVVCAAALLVAILRA
                     RASARRAPSHV"
     gene            complement(2562..3455)
                     /gene="lipA"
                     /locus_tag="P5164_15900"
     CDS             complement(2562..3455)
                     /gene="lipA"
                     /locus_tag="P5164_15900"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011549674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRY45421.1"
                     /translation="MSRPLPVRGEPALPPWIRERKVRLSGLHELKKLMRTRGLHTVCE
                     EARCPNRTECFERKTATFLVCGDVCTRACGFCNVASGRPAALDPEEPANVARAVAEMG
                     LEHVVLTSVDRDDLPDGGAAHWVRVIEAVKALEPRRAVEVLTPDFQGDETAIDLVADA
                     RPDVFNHNVETVPRLYPTVRPKAVFERSVALLARVKERQPGTVTKSGLMLGLGEEDAE
                     VLEVFRALRAAGVDVVTVGQYLRPAAWNLPVVSYATPERFESLRAAGEALGFSYVFAG
                     PFVRSSYRAEEALAESARRLS"
     gene            complement(3452..4105)
                     /locus_tag="P5164_15905"
     CDS             complement(3452..4105)
                     /locus_tag="P5164_15905"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRY45422.1"
                     /translation="MTEEKDGPVHYQVSVTGRQAAAFFLVLLAALGLSFFFGMKTGAA
                     AKRGPDAVTALAAQSDLPVRLEADEGSDASPTPLPTEAPLGFSSPGPARAKEPAPTPR
                     PEPVVKEEPAPAPVVKELSPEPTAVPKAAPADARGEEGPFWVQLLGTSSAEKADALAK
                     RLKADGFRPDVSLVPGKKGLFRVRIGPYPDRARAEAAMKKAVAAEKLDSKPFLSTGK"
     gene            complement(4102..>4503)
                     /locus_tag="P5164_15910"
     CDS             complement(4102..>4503)
                     /locus_tag="P5164_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRY45423.1"
                     /translation="GQAALDELLSEREKLNESLQEILDKHTDPWGIKVTMVEVKAVDL
                     PIEMQRAMAKQAEAEREKRAKIIHASGEFEASKQLAEAAHVIATEPASMQLRYLQTLT
                     EIAAEKNSTILFPVPIEMMALLGQKMAKDVK"
BASE COUNT          619 a         1608 c         1659 g          617 t
ORIGIN      
        1 gggcgaggac gtaggagcgg atctgcgagc cgaagccgat ctccatcttc acgcccgccc
       61 gcttctcctc caccgcccgc ttctcctcga cggcgcgctg gtagaggcgc gacttcagga
      121 ccttcatcgc gaagtccttg ttcttgtgct gcgagcgctc gttctggcag gcgacgacga
      181 gcccggtcgg gaggtgcgtc agccggaccg cggagtcggt cttgttgacg tgctggcccc
      241 ccttgccgcc cgcacggaac gtgtcgatcc ggaggtcctt gtcgaggatc tcgatctcga
      301 tcgtgtcgtc cacctcgggc gtgacgtaga ccgaggcgaa cgaggtgtgg cggcgagccg
      361 ccgcgtcgaa cggagagatc ctcacgagac ggtggacgcc gtgctcggcc ttcatgtggc
      421 cgtaggcgta ctccccctcg aagcggatcg tggcgctctt gatccccgcc tcgtccgcgt
      481 aggagacctc gaggacgtcg gtcttccacc ccttccgctc gccgtagcgg aggtacatcc
      541 gcatcagcat ctcggcccag tcctgggcct ccgtgccgcc tgtgccggcg ttgatctcga
      601 ggagcgccgg gagctcgtcc tccggctccg tcagcttgaa gcgcagctcg cgctcgcgcg
      661 cccaggacgc gatcgaggag agcgcggtgt cggcgtccgc ttccacggcc tccccctccc
      721 ggaggaactc cgcgaggatc tcgagctctt cgcgcttctg gcggaacgcg gtgtcgtccg
      781 agaggaccgt gtcggccctc ttcagcttct tcagcgtctc ctgggccgcg ggagcgtcgt
      841 tccagaagtc gggcgcgccg gcgcgcgcct cgagctcggc cttctccttc tccgcccggt
      901 ggacgtcaaa gatacccccg gagcgcctcg acctgcgtcg cgagcgccgt cagcttctcg
      961 atccgttcct cgttcatgtg cgggggtcct tcctggtcag acgtgggagg gcgcgcggcg
     1021 agccgacgcc cgggcgcgga ggatagccac gaggagcgcc gcggcgcaaa cgacggccgg
     1081 ggcgtcgccg aggcgcgtcg cgagcgtcgt ctcgccgcgg ggcgggacgc ggagcgtccc
     1141 cgcgatgccg gcgcgtgcgc cggggccgag ccgggcgagg gttcgcccgc gcgcgtcgac
     1201 ccagccgctg atccccgtca ccgccgcgcg gagcatcggg cggcccgtct ccacggcccg
     1261 gaagacggcc gcctgccagt gctgctcctg ggcgccgccg tcgccgtacc aggcgtcgtt
     1321 cgtcagcgtg aagagcgcgt cggcgccggc ggcgacctgc gcccgcgtga tccacgggta
     1381 gacgacctcg tagcagatcg ccccgccgag gcgcacgccg cctcgctcca gcgggatcgc
     1441 ccgcgtcccc ggagtgaagg cgccgggcgc ctcccggctc acctggttca cccagccgaa
     1501 aagggacggc gccggcacgt actcgccgaa cgggacgagg cggagcttgt ggtacggctc
     1561 cccgacgggg ccgttgccgt cgacgaggag cgccgagttg aagtacggag cggcggcgtc
     1621 gaggggggcg tcggaccaga ccgtgttgaa gaggagcgcg gtccgctcct cgcggcagag
     1681 gcgcatgagg tcttcgcgca gcccgtcgga ccagggccag gcgaacggcg tcgcgctctc
     1741 cggccagagg acgaggtcgg ccggcgcctt ccgaaggagg gcccgcgtca ggccggtgac
     1801 gtcgaagtgg atccgctcgg cggcccccgg agcgtacctc acgtcctgcg ccacgttcgg
     1861 ctggacgatc aggacgcgga gcgcgcggtc gcgcgcctcc ggcgacggac gggagagccg
     1921 ccacgccccg aagccggtcg ccccgagaag gaccgccgcc gccacggtgg cgctcgcgag
     1981 ccgcgctcgc cccttcgcgc ggatcgcctc ggcgagggcg gcgttcacga ggacgacgag
     2041 gaacgagacg agcgcggccc cgccgagggc cgccgtctgc gtcaggaccg gccacggcgc
     2101 gagagggtag gaaacgaggg cccacgggaa cccggagaag gcgtacgtcc ggaggagctc
     2161 ctgggcggcc cacgcggccg ggaggacgag gagcctcgcc gcggccgtac gcggcgcggc
     2221 cagggccgcg gcccagccca tcgcggcgaa cgcgacgctc gggatacagg cggcgatcag
     2281 gagcgccagg gaggcgagcg gcgccgagag cccgccgaag cggtggacga cgtggaagat
     2341 ccacgggaac gtcgtcagcc agaaggcgac gccgaagagc ccgccgagga gggcgccccg
     2401 gccgggcgag gcgccgtgaa gggcgaggcc gaacgggacg atcgcgaccg gcaggaggag
     2461 gacgagcgaa gcgttcggga acgtcaggcc gaggaggagt ccggagacga gcgccgccgc
     2521 ggcccgcgcg cgaaagggtc cgaagggaaa ccccatcccg ctcaggagag gcggcgcgcc
     2581 gactcggcga gcgcctcctc ggcccggtag ctcgaacgga cgaacggccc ggcgaagacg
     2641 tacgagaagc cgagcgcttc gcccgcggcg cggagcgact cgaagcgctc gggcgtcgcg
     2701 tacgagacga ccgggaggtt ccaggccgcg ggccggaggt actgccccac cgtcacgacg
     2761 tccacgcccg cggcgcggag cgcgcggaag acctcgagga cctccgcgtc ctcctcgccg
     2821 aggccgagca tcaggcccga cttcgtcacc gtccccggct gccgctcctt cacgcgcgcc
     2881 aggagcgcga cggagcgctc gaagacggcc ttcggcctca ccgtcgggta gaggcgcggg
     2941 accgtctcga cgttgtggtt gaagacgtcg gggcgggcgt cggcgacgag gtcgatcgcg
     3001 gtctcgtccc cctggaagtc gggcgtgagg acctcgaccg ccctcctcgg ctcgagcgct
     3061 ttcaccgcct cgatgacccg cacccagtgg gccgccccgc cgtccgggag atcgtcgcgg
     3121 tcgaccgacg tgaggacgac gtgctcgagc cccatctcgg cgacggcgcg tgccacgttc
     3181 gccggctcct ccgggtcgag ggcggcgggc cggcccgacg ccacgttgca gaagccgcac
     3241 gcgcgcgtgc agacgtcgcc gcagacgagg aacgtcgcgg tcttccgctc gaagcactcg
     3301 gtccggttcg ggcagcgcgc ctcctcgcag acggtgtgga gtccccgcgt ccgcatcagt
     3361 ttcttcagct cgtggagccc ggagagccgg accttccgct cgcggatcca cgggggaagc
     3421 gcgggctcgc cccgcaccgg gagggggcgg ctcatttccc ggtcgagagg aacggcttcg
     3481 aatcgagctt ctcggcggcg acggccttct tcatcgcggc ctcggcccgc gcccggtcgg
     3541 ggtagggtcc gatcctgacg cgaaagaggc ccttcttccc ggggacgagg gagacgtcgg
     3601 gacggaaccc gtcggccttc agcctcttgg cgagcgcgtc ggccttctcg gcgctgctcg
     3661 tgccgaggag ctggacccag aacggcccct cctcgccgcg tgcgtcggcc ggcgccgcct
     3721 tcggcacggc cgtcggctcc ggcgagagct ccttcacgac cggcgccggc gcaggctcct
     3781 ccttcacgac cggctcgggg cgcggtgtcg gagcgggctc tttcgcccgg gccgggccgg
     3841 gcgaggagaa gccgagcgga gcctccgtcg gaaggggcgt cggcgacgcg tccgagccct
     3901 cgtccgcctc cagccgcacg ggaaggtccg actgcgccgc gagcgccgtt acggcgtccg
     3961 gcccccgctt cgccgccgcc cccgtcttca tcccgaagaa gaacgagagg ccgagggcgg
     4021 cgaggaggac gaggaagaac gccgcggcct gccggcccgt caccgagacc tggtagtgga
     4081 ccggcccgtc cttctcctcg gtcacttcac gtccttcgcc atcttctggc cgaggagggc
     4141 catcatctcg atcggcaccg ggaagaggat cgtcgaattc ttctcggcgg cgatctcggt
     4201 cagggtctgg aggtagcgca gctgcatgga ggcgggctcc gtggcgatga cgtgggccgc
     4261 ctcggcgagc tgcttcgacg cctcgaactc gccggaggcg tggatgatct tggctcgctt
     4321 ctcgcgctcg gcctcggcct gcttggccat cgcccgctgc atctcgatcg ggaggtcgac
     4381 ggccttcacc tcgaccatgg tcaccttgat cccccacggg tcggtgtgct tgtcgaggat
     4441 ctcctggagg ctctcgttca gcttctcgcg ctcggagagg agctcgtcga gggcggcctg
     4501 acc
//
LOCUS       DAOTEZ010000365         4490 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_14109_length_4490_cov_5.133709, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000365 DAOTEZ010000000
VERSION     DAOTEZ010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14109_length_4490_cov_5.133709"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /gene="trpA"
                     /locus_tag="P5164_15915"
     CDS             <1..680
                     /gene="trpA"
                     /locus_tag="P5164_15915"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRY45424.1"
                     /translation="GADVIELGVPFSDPIADGPAIQRASERGLAAGASLSAVLDLVAR
                     SRASGLDVPLVLFGYANPVLSMGEAVFAARAAAAGVDGALVTDLPPEEGAAFAATLKA
                     ARLDPVFLLAPTSPPKRAKTVGRLSRGFVYVVSRAGVTGVRADVPSGLEELVGRVRDG
                     VGRLPVAVGFGISTPAQVAAVSCLADGVVVGSAVVLSMEDAVRGGADPVEAAAALVRA
                     LAAATSR"
     gene            688..1488
                     /locus_tag="P5164_15920"
     CDS             688..1488
                     /locus_tag="P5164_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45425.1"
                     /translation="MNEPRRDGPEPGSFTAAISRADRASLAAFVEENLPSLGEAEVLR
                     CLRHPYAGEAVIALVLEAPALLASREVRKAIVLHPGTPRPDALRLLEDLPWRDLVDVG
                     RESRTPMPVRHAANRRLLEALPRLSRGERTAIARLADRALAGALLDDGDVDVLAALLG
                     NPRIVEEDLTAWLLVRQPKEAQLALLARTARWMERTAVRSALLLSRRTPRALALSLLS
                     SSERPVWERLAGDPAADPLLAACARSLLEEAGRARGRVERPQRADGGI"
     gene            1538..2395
                     /locus_tag="P5164_15925"
     CDS             1538..2395
                     /locus_tag="P5164_15925"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY45426.1"
                     /translation="MAVETPQQFGEELRRERELREVTREQLADVTKVSVRQIEALETG
                     RFEQLPARVFARGFVLALARHLGLDAERTAAAFNHVHEEWSVEREKSAASTLASSSDR
                     IRLSRPRKNVSINTTALGVGVALVVAVLTGAAALLKGRTGEPAEEAVRSAAPAEAATP
                     VPQALGLPPEVAAATVPLAADRPLVDPAVARSAGSSAEEGRTLTLTFSDDCWTEVSVD
                     GRIVAAELFRKGTRRQFEGGRTFVLTLGNAGGVTVDVDGAALGAVGQPGQVVRNYVIG
                     EGADASRDG"
     gene            2427..3476
                     /locus_tag="P5164_15930"
     CDS             2427..3476
                     /locus_tag="P5164_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45427.1"
                     /translation="MSADAPELDLLAAIESGQAPRRILEFAARGFVPLAPADLVRAVG
                     SILASADPELAPLCEETFRGFDVESLHGAVRSEGIRTEQLDAIARRSKERRVLETVLQ
                     HRAVSDATLVWLARRIPPELQDVLVTNQARLLAAPAIVENLFENPDLSADIRRRADEF
                     LEEFFLKKERERGGRIEEAEEIADAEEIADAEEIADAEEVAEKGRAAAAPEEESGDVL
                     FRLGSLTVAEKIRVAYRGNREERLFLVRDTNRLVSTAVLKSPKTNEADAEVIANMKSV
                     SEDVLRAIAQKREWMKKYSLMSAIVKNPRSPIDVTLALVTRLSQRDQTTLSTDRNVPE
                     AVRVTAKRIVQRRQL"
     gene            3596..4246
                     /locus_tag="P5164_15935"
     CDS             3596..4246
                     /locus_tag="P5164_15935"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRY45428.1"
                     /translation="MTNYYEILGVSRDSSDEVIRNRFRALARDAHPDRVSDPAKKAEA
                     EARFQLLTEAVNVLTNGQRRKAHDFDLDKGAGASTFDPAAIAKVYLAKGVKAYREGDL
                     ATAFQQFDLSVHHWNKDAKALHYLALVCSRIPGKERRGVEAVEGALRLDGENGAVHRE
                     AARLYLQVGLRTKAERHLQEALRRIPEDGEVQRLAAELKPSSENRGLLGGLFGRKG"
     gene            4256..>4490
                     /locus_tag="P5164_15940"
     CDS             4256..>4490
                     /locus_tag="P5164_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HRY45429.1"
                     /translation="MALTAYGLTDVGRKRKHNEDAYLLDAERGLFVVADGMGGHAAGE
                     VASRLTVESIQEFIAGTEDDHDNTWPFGYNNRYS"
BASE COUNT          618 a         1651 c         1613 g          608 t
ORIGIN      
        1 ccggcgccga cgtgatcgag ctcggcgtgc cgttctccga cccgatcgcg gacgggcccg
       61 cgatccagcg ggcgagcgag cggggcctcg cggccggagc gagcctctcc gccgtcctcg
      121 acctcgtcgc gcggtcgcgg gcctccggtc tcgacgtgcc cctcgtcctc ttcggctacg
      181 cgaaccctgt cctctcgatg ggcgaggccg tgttcgccgc gcgcgccgcg gccgcgggcg
      241 tcgacggggc cctcgtgacc gacctcccgc cggaggaggg cgccgccttc gcggcgacgc
      301 tgaaggctgc gcgcctcgac ccggtcttcc tcctcgcgcc gacctcgcct ccgaagcggg
      361 cgaagacggt cggccggctc tcgcgcggct tcgtctacgt cgtctcgcgc gccggcgtca
      421 cgggcgtgcg agccgacgtg ccgagcggtc tcgaggagct cgtcgggcgc gttcgcgacg
      481 gagtcgggcg gctcccggtc gccgtcggct tcggcatctc gaccccggcc caggtcgccg
      541 cggtttcctg cctggccgac ggagtcgtcg tcgggagcgc cgtcgtcctc tcgatggagg
      601 acgccgtgcg tggcggcgcc gatcccgtcg aggcggccgc ggcgctcgtg agggcgctcg
      661 ccgccgcgac gagccgataa tgaccgggtg aacgagcccc gccgggacgg gccggagccc
      721 ggctccttca ccgccgcgat ctcgcgtgcg gaccgggcct ccctcgccgc gttcgtggag
      781 gagaaccttc ccagcctcgg cgaggcggag gtcctgcgct gcctgcggca cccgtacgcc
      841 ggggaggccg tgatcgcgct cgtcctcgag gcgcccgcgc tcctcgcgag ccgcgaggtc
      901 cggaaggcga tcgtcctcca tccggggacg ccgcgccccg acgcgctccg cctcctcgag
      961 gacctcccct ggagggacct cgtcgacgta gggcgcgagt cgcgaacgcc gatgccggtc
     1021 cgccacgccg cgaaccggcg cctgctcgag gcgcttccca ggctctcgcg cggggagcgg
     1081 acggcgatcg cgcgcctggc ggaccgggcc ctcgcgggcg ccctgctcga cgacggggac
     1141 gtcgacgtcc tcgcggctct gctcgggaac ccgaggatcg tggaggagga cctgacggcc
     1201 tggctcctcg tccggcagcc gaaggaggcg cagctcgcgc tcctcgcgcg taccgcgcgc
     1261 tggatggagc ggacggccgt ccgctcggcg ctcctcctgt cgcggaggac gccgcgcgcg
     1321 ctcgcgctct ccctcctgtc ctcctcggag cggcccgtct gggagcggct cgcgggcgat
     1381 ccggcggccg acccgcttct cgcggcgtgc gcccggtccc tcctggagga ggcgggccgc
     1441 gcccgcggcc gcgtcgagcg gccccagcgg gccgacggag gaatttgaca cctctcccgc
     1501 tggaaatgta atctcttctg ccccggaggt ttatcggttg gccgtcgaga ccccccagca
     1561 attcggagag gagctgcgca gggagcgcga gctccgggag gtcacgcgcg agcagctcgc
     1621 ggacgtgacg aaggtctcgg tccgccagat cgaggcgctc gagaccgggc gtttcgagca
     1681 gcttcccgcg cgagtcttcg cgcgaggatt cgtcctcgcc ctggcgcggc acctcgggct
     1741 cgacgccgag cggaccgccg ccgcgttcaa ccacgtccac gaggagtggt ccgtcgagcg
     1801 cgagaagagc gccgcttcga cgctcgcgtc ctcctccgac cggatccgac tctcgcgtcc
     1861 gcgcaagaac gtctcgatca acacgacggc cctcggcgtc ggcgtcgcgc tcgtcgtcgc
     1921 cgtcctgacg ggagcggccg cgctcctgaa gggccggacg ggtgagcccg cggaggaggc
     1981 cgtgcgttcc gcggccccgg cggaggccgc gacgcccgtc ccgcaggccc tcggccttcc
     2041 gcccgaggtc gccgcggcga cggtcccgct cgccgccgac cggcccctcg tcgacccggc
     2101 cgttgcccgt tccgcgggct cgtccgcgga agaggggcgc acgctcacgc tgacgttcag
     2161 cgacgactgc tggacggagg tgagcgtcga cggacggatc gtcgcggcgg agctcttccg
     2221 caaggggacc aggcggcagt tcgagggggg ccggacgttc gtcctgacgc tcggcaacgc
     2281 cggcggcgtg accgtcgacg tggacggcgc cgccctcggc gccgtcgggc agccgggcca
     2341 ggtcgtccgg aactacgtca tcggcgaagg cgccgacgca tcgcgggacg gctgaccgga
     2401 ccgccctggc gtaagctccc tcccgcatgt ccgcggacgc ccccgagctg gacctcctcg
     2461 ccgcgatcga gagcggccag gccccccgcc gaatcctcga gttcgccgcc cggggcttcg
     2521 tgccgctcgc acccgcggac ctcgtccggg ccgtcggttc gatcctcgcg tccgcagatc
     2581 ccgagctcgc cccgctttgc gaagagactt ttcgcggctt cgacgtggaa tcgctccacg
     2641 gcgccgtccg ttcggagggg atccggaccg agcagctcga cgcgatcgcc cggcggtcga
     2701 aggagcgccg cgtcctggaa acggtcctcc agcaccgggc ggtctcggac gccacgctcg
     2761 tctggctcgc ccgacgcatc ccgcccgagc tccaggacgt tctcgtcacg aaccaggcgc
     2821 gcctcctcgc cgctcccgcg atcgtcgaga acctcttcga gaaccccgac ctctcggccg
     2881 acatccgccg gcgagccgac gagttcctgg aggagttctt cctcaagaag gagcgcgagc
     2941 ggggggggcg aatcgaggag gccgaggaga tcgccgacgc ggaggagatc gccgacgccg
     3001 aggagatcgc cgacgccgag gaggtggcgg agaaggggcg tgcagcggcc gcgcccgagg
     3061 aggagtcggg cgacgtgctc ttccggctcg gctcgctcac cgtggccgag aagatccggg
     3121 tcgcctaccg ggggaaccgg gaggagcggc tcttcctcgt gcgcgacacg aaccggctcg
     3181 tctccacggc cgtcctgaag tcgccgaaga cgaacgaggc cgatgccgag gtcatcgcga
     3241 acatgaagag cgtctcggag gacgtcctcc gggcgatcgc ccagaagcgc gagtggatga
     3301 agaagtactc gctgatgtcc gcgatcgtga agaacccccg ctcgcccatc gacgtgaccc
     3361 tggcgctcgt cacgaggctc tcccagagag accagacgac gctgtcgacc gaccggaacg
     3421 tcccggaggc ggtccgtgtc acggcgaagc ggatcgtcca gcgccgccag ctctgaggct
     3481 tttcggacgg gtgccgggcg ccgacacggc gcttgagttt ggcgtgagga gcatgtgaga
     3541 atttcgtcaa cgtctgcgca agggggctcg gtccgcgcaa gctgacggga aagccgtgac
     3601 gaactactac gagatcctcg gggtgagccg cgactcctcg gacgaggtca tccgcaatcg
     3661 cttccgcgcg ctcgcccggg atgcgcaccc ggaccgcgtc tcggaccccg cgaagaaggc
     3721 cgaagccgaa gcgcgcttcc agctcctgac ggaggcggtc aacgtcctga cgaacgggca
     3781 gcgccgcaag gcgcacgact tcgacctcga caagggggcc ggcgcgtcca cgttcgaccc
     3841 ggcggcgatc gcgaaggtct acctggccaa gggggtcaag gcctaccgcg agggggacct
     3901 ggccacggcg ttccagcagt tcgacctctc ggtccaccac tggaacaaag acgccaaggc
     3961 gctgcactac ctcgccctcg tctgcagccg catcccgggg aaggagcgga ggggcgtcga
     4021 agcggtcgag ggcgccctcc gcctggacgg cgagaacggc gccgtccacc gggaggcggc
     4081 ccgcctctac ctccaggtcg gcctccggac gaaggcggag cgccacctgc aggaggccct
     4141 ccggcggatc cccgaggacg gggaggtcca gcgactcgcc gcagagctca agccgtcctc
     4201 cgagaaccgt gggctccttg gcgggctgtt cgggcggaag ggctagaatc ccgccgtggc
     4261 tctcaccgca tacggtctca ccgacgtcgg ccggaagagg aagcacaacg aggacgccta
     4321 cctgctcgac gccgagcgcg gcctcttcgt cgtcgccgac gggatggggg ggcacgccgc
     4381 tggcgaggtc gcttcccgcc tgacggtcga gtcgatccag gagttcatcg ccggcacgga
     4441 ggacgaccac gacaacacgt ggccgttcgg gtacaacaac cgctactccg
//
LOCUS       DAOTEZ010000366         4484 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_14138_length_4484_cov_8.040415, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000366 DAOTEZ010000000
VERSION     DAOTEZ010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14138_length_4484_cov_8.040415"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..427
                     /locus_tag="P5164_15945"
     CDS             155..427
                     /locus_tag="P5164_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45430.1"
                     /translation="MVQDGNGLRFLLEPTGGRESVRLRNLVLARLRRDEAGNETARDI
                     ARTSLNLRMREPFVLGIGREEGGGAALFLVLTADPVESGPGIAGRR"
     gene            427..1662
                     /locus_tag="P5164_15950"
     CDS             427..1662
                     /locus_tag="P5164_15950"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRY45431.1"
                     /translation="MPEFVCRIGTPDGSVVTRTVEASDEAALRADLARQGSRLFSAKA
                     GNGRGAPAASLGARLGSFRSRGRVGVHEFLVFNQELVALLRAGLPVVSGFEILLERQE
                     NAHFKRILIDVREQLVSGVALSDAFLSHGDTFPRLYATSLKAGERSGEVEKVLRRFLD
                     YQKILGRVKRRVTGAMAYPIVLVSLAVGLIFILMTYVIPKFTEFYAGFGADMPALTVA
                     VVGTATFMRAHLLEILLGTAAAVFAFDRWRRTDAGQRSIDGALLKLPAVGVVLHEFSL
                     SQFARALATLVGAGTPLVTALEISTGSVGNRRVSDAVASVVPKVREGAELWRSLEQTG
                     QFTSLAVEMIKVGEATGALEEMLTNVSDFYDEAIEARLQTIINLIEPVILIVMGGVIA
                     TILLSVYLPMFTILSSIRG"
     gene            1666..3297
                     /locus_tag="P5164_15955"
     CDS             1666..3297
                     /locus_tag="P5164_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRY45432.1"
                     /translation="MTTNDAPAVPAFPRVLDAASEESRAKELGRRLGIPYVDLWTFRV
                     DPALFKSVPLEWMLRHEFVPESEEDGVLAVVLTDPTDVVRRDELEALLRRRVRVKVGS
                     RAAMQEILQKSESTQRVLEEATEEFRMQVVREDEEGQEVLTIDRIAADASPIIKLVDS
                     VLFNAIQRRASDIHIETQENVVVIKYRIDGVLYPAMEPIDKKHHQTIVSRIKVMSELD
                     IAEKRIPQDGRFKIRVKGRTIDFRVSIMPTVHGEDVVIRILDKESANAEFKNLRLDVL
                     GMDAETMKRLRKFIREPYGMVLVTGPTGSGKTTTLYACLSEIQSVEDKIVTIEDPVEY
                     QLRGITQIPVNEKKGLTFARGLRSILRHDPDKVMVGEIRDEETAQIAVQAALTGHLVF
                     TTVHANNVVDVLGRFLNMKVELYNFVSALNCILAQRLVRKICPRCKTPMEPTKQLLDE
                     SALTAAEVANVVFYEGRGCIECNGTGFYGRMAITELLDLSDRIRGLILDRRPAAEIKK
                     AAKEEGMAFLRESALRKVFLGETTLKEINKVTFVD"
     gene            3287..4249
                     /locus_tag="P5164_15960"
     CDS             3287..4249
                     /locus_tag="P5164_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45433.1"
                     /translation="MSTDGLLARVGRALAGSWGYRRPPHAFVLTEERLVHVAAPREAR
                     AGGGLAVRSRELPPGTFQDAPSGGRVAGPGLAQAVAALLPPNQHLAAASLAVPDGFVK
                     AAPVDVEPGVEKNPKELAEVLRWKVGRLWGEPAPALRIGWCRAGEASGGAPRFLVVSA
                     PEETVASCEAAFAARGIRIGALEPASLALSALAAKALPGSGFLVFADGATVSTVWLDD
                     GAPRFLRTRAATADPDEALQEFRLAATFVGGADVEGPGPDLSADVIAVPEASDLAGRL
                     RELRRENGGKEPVSLLAYLAGRGLPVRAGDTAPLVGLGLLEGAE"
     gene            4252..>4484
                     /locus_tag="P5164_15965"
     CDS             4252..>4484
                     /locus_tag="P5164_15965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45434.1"
                     /translation="MAAPRPNLARRPFVDTRPANLVAALLLAAAIGLTIVSVRTVRSY
                     VEGSRTSREAIAGLRSEIGRYEQARREAEAKLAR"
BASE COUNT          640 a         1658 c         1538 g          648 t
ORIGIN      
        1 tgtcgaggcc gtagaggagg aaccgggcgt cggccatccg ggccagggtt tcgccgcctt
       61 gctggcgccg gatgccctcg agcacctcct ggtacacccg ccaagccacc cccgcgaggg
      121 gaccgcgcgg catcggccgc gccgtctcga gctggtggta caggacggaa acggcctccg
      181 gttcctcctc gagccgacgg gagggcggga gagcgtccgc cttcggaacc tggtcctcgc
      241 ccgcctgcgg cgcgacgaag ccgggaacga gacggcccgc gacatcgccc gcacctccct
      301 caacctccgg atgcgcgagc cgttcgtcct cggcatcggg cgcgaggagg ggggcggcgc
      361 ggcgctcttc ctcgtcctga ccgccgatcc ggtcgagtcc ggtcccggga tcgcggggcg
      421 gcgctgatgc ccgagttcgt ctgccgcatc gggacgcctg acggaagcgt cgtcacccgg
      481 accgtggaag cctccgacga ggcggccctg cgggccgacc tcgcgcgcca gggctcgcgc
      541 ctcttctccg cgaaggcggg gaacggccgg ggcgccccgg ccgcgagcct cggagcccgc
      601 ctcggctcgt tccggagccg gggccgggtc ggcgtgcacg agttcctcgt cttcaaccag
      661 gagctcgtcg ccctcctccg ggccgggctc ccggtcgtct ccggcttcga gatcctcctc
      721 gagcggcagg agaacgcgca cttcaagcgg atcctgatcg acgtgaggga gcagctcgtc
      781 tcgggggtgg cgctctcgga cgcgttcctc tcccacggcg acacgtttcc gcgtctctac
      841 gccacctcgc tcaaggcggg ggagcgctcc ggcgaggtcg agaaggtcct ccgccggttc
      901 ctcgactacc agaagatcct cggccgggtg aagcggaggg tgaccggggc gatggcctac
      961 cccatcgtcc tcgtgagcct tgccgtcggc ctgatcttca tcctgatgac ctacgtcatc
     1021 ccgaagttca cggagttcta cgcgggcttc ggtgcggaca tgccggccct gaccgtcgcc
     1081 gtcgtcggca cggcgacctt catgcgcgcg cacctcctcg agatcctcct cggcaccgcg
     1141 gcggcggtct tcgcgttcga ccgctggcgg cggaccgacg ccggccagcg gtcgatcgac
     1201 ggggccctcc tgaagctccc ggccgtcggc gtcgtcctcc acgagttctc cctctcgcag
     1261 ttcgcccgcg ccctcgcgac cctcgtcggg gccggcaccc cgctcgtcac cgcgctcgag
     1321 atctcgaccg gctcggtcgg caaccgccgg gtgtcggacg cggtcgcctc cgtcgtgccg
     1381 aaggtgcggg agggagccga gctctggcgg agcctcgagc agacggggca gttcacctcc
     1441 ctcgccgtcg agatgatcaa ggtgggcgag gccacgggcg cgctcgagga gatgctgacg
     1501 aacgtctcgg acttctacga cgaggcgatc gaggcgcgcc tccagacgat catcaacctg
     1561 atcgagccgg tcatcctgat cgtgatgggg ggcgtcatcg cgacgatcct cctgtccgta
     1621 tacctcccga tgttcacgat cctctccagc atccggggct gacgcatgac gacgaacgac
     1681 gcccccgccg tccccgcttt cccgcgcgtc ctcgacgcgg cctccgagga gagccgcgcc
     1741 aaggagctcg ggcggaggct cggcatcccc tacgtcgacc tctggacgtt ccgggtcgac
     1801 cccgcgctct tcaagagcgt cccgctcgaa tggatgctcc ggcacgagtt cgtcccggag
     1861 agcgaggagg acggcgtcct cgccgtcgtc ctgacggacc ctacggacgt cgtccggcgc
     1921 gacgagctcg aggcgctcct gaggcggcgg gtccgcgtga aggtcggctc gcgcgccgcg
     1981 atgcaggaga tcctgcagaa gtcggagtcg acgcagcgcg tcctcgagga ggcgaccgaa
     2041 gagttccgga tgcaggtcgt gcgggaggac gaggaggggc aggaggtcct gacgatcgac
     2101 cggatcgcgg ccgacgccag cccgatcatc aagctcgtcg actcggtcct cttcaacgcg
     2161 atccagcgcc gcgcctcgga catccacatc gagacccagg agaacgtcgt cgtcatcaag
     2221 taccggatcg acggcgtcct ctacccggcg atggagccga tcgacaagaa gcaccaccag
     2281 acgatcgtca gccgcatcaa ggtcatgtcg gagctcgaca tcgccgagaa gcggatcccg
     2341 caggacgggc gcttcaagat ccgggtcaag gggcggacga tcgacttccg cgtctcgatc
     2401 atgccgaccg tccacggcga ggacgtcgtc atccggatcc tcgacaagga gtcggcgaac
     2461 gccgagttca agaacctccg cctcgacgtc ctcgggatgg acgccgagac gatgaagcgc
     2521 ctccggaagt tcatccgcga gccgtacggc atggtcctcg tcaccggccc gaccggctcg
     2581 ggcaagacga cgacgctcta cgcctgcctg tcggagatcc agtccgtcga ggacaagatc
     2641 gtcacgatcg aggacccggt cgagtaccag ctccgcggca tcacgcagat cccggtcaac
     2701 gagaagaagg ggctcacgtt cgcgcggggg ctccgctcca tcctccggca cgaccccgac
     2761 aaggtcatgg tcggcgagat ccgcgacgag gagacggcgc agatcgccgt ccaggccgcg
     2821 ctcacggggc acctcgtctt cacgacggtc cacgcgaaca acgtcgtcga cgtcctgggc
     2881 cggttcctca acatgaaggt cgagctctac aacttcgtct cggccctgaa ctgcatcctc
     2941 gcccagcggc tcgtccggaa gatctgcccg cgctgcaaga cgccgatgga gccgacgaag
     3001 cagctcctcg acgagtcggc gctgacggcg gccgaggtcg cgaacgtcgt cttctacgag
     3061 gggcgcggct gcatcgagtg caacgggacc ggcttctacg gccggatggc gatcaccgag
     3121 ctcctcgacc tctccgaccg gatccgcggc ctcatcctcg accgccggcc cgccgcggag
     3181 atcaagaagg cggcgaagga ggaggggatg gccttcctcc gcgagtcggc gctccgcaag
     3241 gtcttcctcg gggagacgac tctgaaagag atcaacaagg tgacctttgt cgactgacgg
     3301 actcctcgcc cgcgtcggcc gcgccctcgc cgggagctgg ggctaccggc ggccgccgca
     3361 cgccttcgtg ctgacggagg agcggctcgt ccacgtcgcc gcgccgcggg aggcgcgggc
     3421 ggggggcggc ctcgccgtcc gctcccgcga gctcccgccg ggcacgttcc aggacgcccc
     3481 ctccggcgga cgcgtcgcgg ggcccggcct cgcgcaggcc gttgccgcgc tcctcccgcc
     3541 gaaccagcac ctcgcggccg cgtccctcgc cgtcccggac ggattcgtga aggcggcccc
     3601 cgtcgacgtc gagcccggcg tcgagaagaa cccgaaggag ctcgccgagg tccttcgctg
     3661 gaaggtgggg cggctctggg gcgagccggc gccggcgctc cgtatcggct ggtgccgggc
     3721 gggcgaggcg tccgggggcg ctccccggtt cctcgtcgtc tccgcgcccg aggagaccgt
     3781 cgcctcgtgc gaggcggcgt tcgcggcgcg cgggatccgg atcggcgccc tcgagcccgc
     3841 gtccctcgcc ctctcggccc tcgccgcgaa ggcgctcccg ggctccggct tcctcgtttt
     3901 cgccgacggc gcgaccgtct cgaccgtctg gctggacgac ggggctcccc ggttcctccg
     3961 gacccgcgcc gccacggcgg accccgacga ggcgctccag gagttccgcc tcgcggcgac
     4021 gttcgtcggc ggggccgacg tcgaaggccc cggtcccgac ctctcggccg acgtgatcgc
     4081 cgtccccgaa gcttcggatc tcgccggacg gctccgggag ctccggcgcg agaacggggg
     4141 gaaagagccc gtctccctcc tcgcgtacct cgccggccgg ggactccccg tccgcgccgg
     4201 ggacacggcg cccctcgtcg ggctcgggct cctcgaagga gcggagtgag catggccgcc
     4261 ccgagaccga acctcgcccg ccgccccttc gtcgacaccc ggcccgcgaa cctcgtcgcc
     4321 gcgctcctcc tcgccgccgc gatcggcctg acgatcgtga gcgtgaggac ggtccgctcc
     4381 tacgtcgaag gctcgcggac ctcgcgcgag gcgatcgccg ggctgcgctc cgagatcggc
     4441 cgctacgagc aggcccgccg ggaggccgag gcgaagctcg cccg
//
LOCUS       DAOTEZ010000367         4477 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_14164_length_4477_cov_6.750113, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000367 DAOTEZ010000000
VERSION     DAOTEZ010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14164_length_4477_cov_6.750113"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="P5164_15970"
     CDS             <1..788
                     /locus_tag="P5164_15970"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRY45435.1"
                     /translation="YEEGKKIGFKDALQAVWVILKYARWEAPRGDVGTMTLRRMERLA
                     PYNAWLHDRFDAALGQRILEVGSGVGNQTRFFVERERVVASDVEVHYVRELTARFGNR
                     SNVRVASYRFPLADADRAELVAERLDSIVCLNVLEHIEDDSATLRDFASVLPKGGRLV
                     LLVPSLKALYGTLDVHLHHFRRYERDELVKLVSGAGFEVETARFLNRPGVLGWWLNSR
                     VLKRKVLPKGQLRAFRWLLPLLKLEERRLPSFGMSLLVLARRA"
     gene            785..3097
                     /locus_tag="P5164_15975"
     CDS             785..3097
                     /locus_tag="P5164_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45436.1"
                     /translation="MTPGRLARLEPFAAPAALAGAVALRFHEYLLGGSVVGRDAVFFF
                     VPLREVTSRLLSAGELPLWNDASGAGRALAANPNAAAFWPGTWLVPLLGTTGLFLFHV
                     LLVVLLAYAALRALGLSRVASSAGGLVLLFSGVFQTVPLLFTTVASLAPIPLALAAIG
                     TLDPDDGPGARRRVAAVGLALGLSLLGGEPVVAATGAAGTAAVALAGALVDARRGAPG
                     RAGRRLGALAAAGLLSAGIAAVQLLPTAGELARSARGTQMRAEEGSLFWSVRPTRLLT
                     LLEPRLTGDSRAEDDADYWGAATFDAGNPYFHDLALGLLPLALAAAAAGDPRGRSALG
                     LSGLAALLSFGRFFPPVGMLLGALPVFRYPEKWWLLATLGLAGAAAVGVDRLRDDPRA
                     PRRFARASALLAALLLPAAALAFTAPDRLAALLRAVSLAGDGVSGERVGALLGPLLLA
                     ALATALLLASLPALVRSGRISTRAAAGLAAALFLLDAGRRVTGSAPAGPASFWDSVPA
                     EVAAAKEAAGEGRFYVDGADDVARATALARERAGLDPLRPTTGAAWGLRYGGDNDVDR
                     MSTPESVASSATLARMPWGEEKVRRLRTIGVTAARTDAPGPDPSGVRATARFGSARIV
                     VLDGARPEHHFVRRARPAPGALVVPAPSDPLVEAVVAGLGEERSFGAGEVTLLERTNG
                     RRRLRARVAPPGGLLVAARTFDPSWTATVDGRRVESLCADGFLTAVPVPAGEHEVVFA
                     YRNGRMLAGLAITLLALGAAASLAFVGRRG"
     gene            3094..>4477
                     /locus_tag="P5164_15980"
     CDS             3094..>4477
                     /locus_tag="P5164_15980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45437.1"
                     /translation="MRSLHDLFEERPFAVALLVALVLLLVPYRTLLGPGVPSGRDLLP
                     YFYPLKAHLVEALRAGEMPWIDRFRQGGLPLLASPGVAAFDPGNVLFVLLPTPLAAKA
                     WMLLRVLTGAAGFAVFLRLSGLPPLSSALGGLTWGAAGITASAASFLSTSSAHAALPW
                     FAAALLRVRSRRDARSLVLLAAATALLVVASVPEPLLAASLLAVVLLAGRGPAAGLRE
                     RGATLLLWGSGGLLGALLAAPALAALVIAGFESIRGVRGALLPGFAELGSLPASRLAE
                     LLADGVVADWTKTTVAAEISRYPYFPSLTPGRVAWTLAILGLLAGRGARLRASTLAVL
                     GVLLALGPATPVAGWIAKVVPFAASIRYPEKWLVVFGFGIAWLSALGAAAIEKAAPAR
                     RAFVLGVLGLLLASDRALLTPRLLPMSDAAILTERPSVLATLPSARDDEPPPRILPLP
                     ASCLPELAAQA"
BASE COUNT          406 a         1744 c         1612 g          715 t
ORIGIN      
        1 cgtacgagga agggaagaag atcggcttca aggacgccct ccaggccgtc tgggtgatcc
       61 tgaagtacgc gcgttgggag gcgccgcgcg gcgacgtcgg gacgatgacg ctccgccgga
      121 tggagcggct cgccccgtac aacgcctggc tccacgaccg cttcgacgcc gcgctcggac
      181 agcggatcct cgaggtcggc tccggcgtcg ggaaccagac gcgcttcttc gtcgaaaggg
      241 aacgggtcgt cgcctcggac gtcgaggtcc actacgtccg cgagctgacc gcgaggttcg
      301 ggaaccgctc gaacgtccgc gtcgcgtcgt accgcttccc gctcgccgac gccgacaggg
      361 ccgagctcgt cgccgagagg ctcgactcga tcgtctgcct gaacgtcctc gagcacatcg
      421 aggacgactc cgcgacgctc cgcgacttcg cctcggtgct gccgaaaggc ggccgcctcg
      481 tcctcctcgt gccgtcgctg aaggcgctgt acgggacgct cgacgtccac ctccaccact
      541 tccgccgcta cgagagggac gagctcgtga agctcgtctc gggggcgggg ttcgaggtag
      601 agacggcgag gttcctcaac cgtccgggcg tcctcggctg gtggctcaac tcgcgggtcc
      661 tgaagcggaa ggtcctcccg aaggggcagc tccgcgcctt ccggtggctc ctgccgctcc
      721 tcaagctcga ggagaggcgc ttgccgtcgt tcgggatgtc gctcctcgtc ctggcgcgaa
      781 gggcgtgacg ccggggcgcc tcgcccggct cgagcccttc gccgcgcccg cggcgctcgc
      841 cggggcggtg gccctccggt tccacgagta cctcctcggc ggaagcgtcg tcgggcgcga
      901 cgcggtcttc ttcttcgtcc cgctccggga ggtcacgtcg cgcctcctgt ccgcggggga
      961 gctgccgctc tggaacgacg cctccggcgc gggacgggcc ctggccgcga atccgaacgc
     1021 cgcggcgttc tggcccggca cctggctcgt cccgctcctc ggcacgacgg gcctcttcct
     1081 cttccacgtc ctcctcgtcg tgctcctcgc gtacgccgcg ctccgcgcgc tcggtctttc
     1141 ccgggtcgcc tcctctgccg gcggcctcgt cctcctcttc tcgggcgtct tccagacggt
     1201 cccgctcctc ttcacgacgg tggcctcgct cgccccgatc cctctggctc tcgcggccat
     1261 cgggacgctc gacccggacg acgggcccgg cgcacggcgg agggtcgcgg ccgtcgggct
     1321 cgcgctcggg ctctcgctgc tcggcgggga gccggtcgtg gcggcgaccg gcgcggccgg
     1381 caccgcggcg gtcgcgctcg cgggcgcgct cgtcgacgcc cggaggggag ctcccgggcg
     1441 cgccggacgg cgcctcggcg cgctcgccgc ggcggggctc ctctccgccg gcatcgccgc
     1501 cgtccagctc cttccgacgg cgggcgagct cgcccggtcg gcgcggggga ctcagatgag
     1561 ggcggaagag gggtcgctct tctggtcggt tcgaccgacg cgcctcctga ccctgctcga
     1621 gccgcggctg acgggcgatt cgcgcgccga ggacgacgcg gactactggg gcgcggcgac
     1681 gttcgacgcc gggaacccct acttccacga cctcgcgctc ggcctcctgc cgctcgcgct
     1741 cgcggccgcc gcggcgggcg acccgcgcgg ccggtcggcg ctcggtctct caggcctcgc
     1801 cgcgctcctc tccttcggga ggttcttccc gccggtcggg atgctcctgg gcgcgctgcc
     1861 ggtcttccgc tacccggaga agtggtggct tctcgccacg ctcggcctgg ccggagcggc
     1921 ggcggtcggc gtcgaccggc tccgcgacga tccgcgggct ccgcgccgct tcgcgagggc
     1981 gtcggccctc ctcgccgccc ttctccttcc cgccgcggcg ctcgccttca cggctcccga
     2041 ccggctcgcc gcgctgctcc gggccgtgtc gctcgcgggc gacggggtga gcggggagcg
     2101 ggtcggagcg ctgctcggac cgcttctcct cgcggcgctc gcgacggccc ttctcctcgc
     2161 gtcgctgccg gcgctcgtga ggtcgggacg catctccacg cgggcggcgg cgggcctcgc
     2221 cgcggcgctc ttcctcctcg acgcggggcg tcgggtgacc ggatcggcgc cggcggggcc
     2281 cgcgagtttc tgggactccg ttcccgcgga agtagccgcc gcgaaggagg ccgccggcga
     2341 gggacgcttc tacgtcgacg gggcggacga cgtggcgcgg gcgacggcgc tcgcgcgcga
     2401 gcgcgccggg ctcgatccgc tccgcccgac caccggcgcc gcgtgggggc tgcgctacgg
     2461 cggcgacaac gacgtcgacc ggatgtcgac gcccgagtcg gtggcgtcgt cggcgacgct
     2521 cgcgcggatg ccgtggggcg aggagaaggt ccggcgcctc cggacgatcg gcgtgacggc
     2581 ggcgcgaacc gacgcacccg ggcccgaccc ctccggcgtt cgcgcgacgg cgcgattcgg
     2641 gagcgcgcgg atcgtcgtgc tggacggcgc acggcccgag catcacttcg tgaggcgcgc
     2701 gcgtccggcc cccggcgctc tcgtcgtccc ggccccctcg gatccgctgg tggaggcggt
     2761 cgtcgcgggg ctcggcgagg agcgttcctt cggagccggc gaggtgacgc tcctcgagcg
     2821 aacgaacggt cgtcgccgtc tccgggcgcg cgtggcgccg ccgggcggtc tgctcgtcgc
     2881 cgcacgcacc ttcgacccgt cctggacggc gaccgtcgac ggacggaggg tcgagagcct
     2941 ctgcgccgac ggcttcctga cggccgtccc cgtcccggcc ggcgagcacg aggtcgtctt
     3001 cgcctaccgg aacggacgga tgctcgccgg cctcgcgatc acgcttctcg ccctcggcgc
     3061 cgcggcgtcc ctggcgttcg tcgggaggcg cggatgagga gcctgcacga tctcttcgag
     3121 gagcggccgt tcgcggtcgc tctcctcgtc gccctcgtcc tcctcctcgt tccctaccgg
     3181 actctgctcg ggcccggcgt cccgtcgggc cgcgacctcc tgccgtactt ctatccgctg
     3241 aaggcgcacc tggtcgaggc gctccgggcg ggggagatgc cgtggatcga ccgcttccgc
     3301 cagggcggcc tcccgctcct tgcctcgccg ggcgtcgcgg ccttcgaccc cggaaacgtt
     3361 ctcttcgtcc tccttccgac tcccctcgcc gcgaaggcgt ggatgctcct gcgcgtcctg
     3421 acgggggccg cgggcttcgc cgtcttcctc cggctctcgg gcctcccgcc gctctcgtcg
     3481 gcgctcggcg gcctcacgtg gggcgcggcg gggatcacgg cgtccgcggc gagcttcctg
     3541 agcacctcgt ccgcgcacgc ggcgctgccc tggttcgcgg ccgcgcttct tcgggtccgc
     3601 tcgcggcgcg acgcgcgaag cctcgttctc ctcgccgccg cgaccgcgct cctcgtcgtc
     3661 gcgtcggttc cggagccgct cctcgccgcg tcgctcctcg ccgtcgtcct cctcgccggg
     3721 cggggcccgg ccgcgggcct gcgcgagcgg ggggcgacgc tcctcctctg ggggtcgggc
     3781 gggctcctcg gcgcgctcct cgctgcgccc gcgctcgccg ccctcgtcat cgcgggcttc
     3841 gagagcatcc ggggtgttcg cggggcactc ctccccggct tcgcggagct ggggtcgctg
     3901 cccgcgtcgc ggctcgcgga gctgctcgcg gacggcgtgg tggccgactg gacgaagacg
     3961 accgtcgcgg cggagatctc ccgctacccc tacttcccgt cgctgacgcc cggacgcgtc
     4021 gcatggacgc tcgcgatcct cggtctcctc gcggggcgtg gcgcgcgtct ccgggcgtcg
     4081 accctggccg tgctcggcgt cctgctcgcc ctcgggccag cgactcccgt ggccggctgg
     4141 atcgcgaagg tcgtgccgtt cgccgcgtcg atccggtacc cggagaagtg gctcgtcgtt
     4201 ttcggtttcg ggatcgcctg gctctccgct ctcggcgccg cggcgatcga gaaggcggct
     4261 cccgctcgac gggccttcgt gctcggggtt ctcggtctcc tcctcgcttc cgatcgggcc
     4321 ctcctcacgc cgaggctcct gccgatgtcg gacgcggcga tcctgacgga gaggccgtcc
     4381 gttctcgcca cgctgccgtc cgcccgcgac gacgaaccgc ccccgcgcat cctgcccctg
     4441 cccgcatcgt gcctgcccga actggcggcg caggcgc
//
LOCUS       DAOTEZ010000368         4440 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_14338_length_4440_cov_6.581300, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000368 DAOTEZ010000000
VERSION     DAOTEZ010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14338_length_4440_cov_6.581300"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="P5164_15985"
     CDS             complement(<1..650)
                     /locus_tag="P5164_15985"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY45438.1"
                     /translation="MSEPGATASGETPRGTLSRVAAVAALSFSSGLPLGLVWIAIPDW
                     MRSIGVDIRVVGLFTLAQAPWSFKFLWSPLMDRFRLPFWGRRRGWIAVAQGALVLAGL
                     ALSGVGSRPETPWVALAVTLAVALAGATQDIAIDAYAVDVLRKEEHGVAVGLRMALYR
                     AAMTLSGGLAIWAAASLGWGAVNVLLALSYLPLLLVTWRSPEPEEETVAPKTLRDAV"
     gene            834..1883
                     /locus_tag="P5164_15990"
     CDS             834..1883
                     /locus_tag="P5164_15990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY45439.1"
                     /translation="MTRPALVVLSTVLASLLSGCGPDSGAAAPAAPPPQVRTLRLAPE
                     DVVLSTTVVGTLEPSARVVVAAQEEGVVTAVAVREGDRVAAGDLVVQLDDRRIRAELA
                     EAEARLLDAKAQARRAKALEGDGLISPSDADTIRASEAMAEARAELLRTRTSFTRITA
                     PVAGVVLARRVEVGNLASARTPLVELGAGAGLLLRVPVSELEVVHLRPGDAATVKVDA
                     LPDVSMAARIARIFPAAEGTTRQVTVELSLVDPPAAVRPGFLARARMVVEKRKGVLLV
                     PEAALQRGSETPHFVWVLDGDTARVRPVEPGERVDGRVVVRKGLAAGDEVVVEGLVRL
                     RDGGPARRAATESGS"
     gene            1880..>3196
                     /locus_tag="P5164_15995"
     CDS             1880..>3196
                     /locus_tag="P5164_15995"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRY45440.1"
                     /translation="MSGNGERRGLVHWSITRPVGTSIIALAICILGVSMTGRLAVDLL
                     PRIVYPQIGARVSNPGVDPEVMEQTVTKLLERRLATTENAILITSESSEGDSDVDIHF
                     SYGTDVDVALRDASTKLDQARSALPEEAEPPSIGKRDPSQIPVLNFAVSSPTRDEGWL
                     KRWCEDQLSRQLLTVEGVASVDVAGGLDREIQVRLDPERLRSYRLTVSEVLTRVREEN
                     QDIAAGRLQSTSRDVVSKTKGKFTSPADIAAVRLPLPGGGDVALSDVAEVRDTHLDDR
                     VFARLDGKPAVQVAISKQPDANTVQVVDGCNRVLERLKRDGFFPPDVITQVTQDQAFF
                     VRASVSGVGEAAALGGGLAMLVVFLFLRSVRRTLIIGTAIPISILGCVALMGTSGLTL
                     NIMSLGGLALGIGMLVDNAIVMLENIDRHQREHPDPVEAAHVGAGEV"
     assembly_gap    3197..3296
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3297..>4440
                     /locus_tag="P5164_16000"
     CDS             <3297..>4440
                     /locus_tag="P5164_16000"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRY45441.1"
                     /translation="RLGNEFLPQVDDGRFRVSIELPVGTPVDVTNRATLAAEKVVREL
                     PAVRHVFAAAGGGIWGRGMWVRPRFASMEVEVEPRRSRGMTASAWLQRAQAELSALPE
                     LADARVRVQPPRIRGLRTSTGTEDLEVKVFGDDLPTLQRLGEEVEARLGKLSGLSNVE
                     TSLKETAPELRVVVDRRRAADLGLDVGEVGRTVRTAVGGSVATRLTEGDREFDVRVRF
                     DPAKVRDASEMASLPLFPRSGAALRLRDVADVREAAAPQTIQRENQNRLVTVGASVLP
                     QVWSISEATAEARKALADLKLPDGYTVRFGGQQEAVDENRRVLVTVVLLAVFLVFAVM
                     AVQYESLVNPLVIMAAIPLALVGVVLALTLTGLPMSAPVMLGVILLA"
BASE COUNT          563 a         1630 c         1587 g          560 t
ORIGIN      
        1 accgcgtcgc gcagcgtctt cggggcgacg gtctcctcct ccggctccgg cgaccgccag
       61 gtcacgagga ggagcgggag gtaggagagc gcgaggagga cgttgaccgc gccccagccg
      121 aggctcgccg ccgcccagat cgcgaggccg cccgagaggg tcatcgcggc ccggtagagc
      181 gccattcgca ggccgacggc caccccgtgc tcctccttcc ggaggacgtc gacggcgtag
      241 gcgtcgatcg cgatgtcctg cgtcgcgccc gcgagcgcca cggcgagcgt gacggcgagg
      301 gcgacccagg gcgtctcggg acggctcccg acgccggaga gggcgagccc cgcgaggacg
      361 agcgccccct gcgccacggc gatccagccc cgccgccggc cccagaacgg cagccggaac
      421 cggtccatga gcggcgacca gaggaacttg aaggaccacg gggcctgcgc gagggtgaag
      481 aggccgacga cgcggatgtc gacgccgatc gagcgcatcc agtcggggat cgcgatccag
      541 acgagcccga gcggcagccc ggaggagaac gacagcgccg cgaccgccgc gacccgcgac
      601 aacgtccccc gcggggtctc tcccgacgcc gtcgcgcccg gctcgctcat ggacgggcga
      661 ttgtacgggc aggccgagac cgcgacgcgg ccggaatacc cggcgcccac gcgccgttct
      721 cagccgggtg accgatccgc ttcgacaccc ccgagccgcc ctgctagcct gtccggacgc
      781 cgcgcccgcg ccgcgcccgc cacgggggcg gtgaggagcg gcgcggaggc ccgatgaccc
      841 gtcccgccct cgtcgtcctg tcgaccgtcc tcgcgtccct cctttccggc tgcggacccg
      901 actccggcgc ggccgcgccc gccgccccgc cgccccaggt gcggacgctg cgcctcgccc
      961 cggaggacgt cgtcctctcg acgaccgtcg tcggcacgct cgagccttcg gcgcgcgtcg
     1021 tcgtcgcggc gcaggaggag ggcgtcgtca ccgccgttgc cgtccgcgag ggggaccggg
     1081 tcgccgcggg ggacctcgtc gtgcagctcg acgaccggag gatccgggcc gagctcgcgg
     1141 aggcggaggc gcgccttctc gacgcgaagg cgcaggcccg acgcgcgaag gcgctcgagg
     1201 gagacggcct catctcgccg tccgacgccg acacgatccg ggcctccgag gcgatggccg
     1261 aggcgcgggc cgagctcctc cgcacgcgga cctcgttcac gcggatcacg gcccccgtcg
     1321 ccggcgtcgt cctcgcgcgg cgcgtggagg tggggaacct cgcctccgcg cgcacgccgc
     1381 tcgtcgagct gggggccggc gcgggcctgc tccttcgcgt gccggtctcc gagctggagg
     1441 tcgtccacct ccggcccgga gacgcggcga cggtcaaggt cgacgcgctc ccggacgtct
     1501 ccatggccgc ccggatcgcc cggatcttcc cggccgccga ggggacgacg cggcaggtga
     1561 cggtcgagct ctctctcgtc gatcccccgg ccgccgtccg gcccgggttc ctcgcgcggg
     1621 cccgcatggt cgtcgagaag cggaagggag tcctcctcgt ccccgaggcg gcgctccagc
     1681 gcgggtcgga gacgccccac ttcgtctggg tcctcgacgg cgacacggcg cgggtccgtc
     1741 ccgtcgagcc gggcgagcgg gtggacgggc gggtcgtcgt ccgcaagggc ctcgcggccg
     1801 gcgacgaggt cgtcgtggag gggctcgtcc gcctgcgcga cggcggcccc gcccggcgtg
     1861 cagccacgga gagcgggtcg tgagcgggaa cggggagcgc cggggcctcg tccactggtc
     1921 gatcacccgc cccgtcggca cgtcgatcat cgccctcgcg atctgcatcc tcggcgtgtc
     1981 gatgacgggg aggctcgcgg tcgacctgct gccgcgaatc gtctacccgc agatcggggc
     2041 ccgggtgagc aaccccggcg tcgacccgga ggtgatggag cagacggtca cgaagctcct
     2101 cgagcgcagg ctcgcgacga ccgagaacgc gatcctgatc acctcggagt cgtccgaggg
     2161 ggacagcgac gtcgacatcc acttctccta cgggaccgac gtcgacgtcg ccctgcgcga
     2221 cgcgtcgacg aagctcgacc aggcccggtc ggccctcccc gaggaggccg agccgccgtc
     2281 gatcggcaag cgggacccct cgcagatccc cgtcctgaac ttcgccgtct cctcgcccac
     2341 ccgggacgag gggtggctca agcgctggtg cgaggaccag ctctcccgcc agctcctgac
     2401 cgtcgaaggg gtcgcctcgg tcgacgtggc gggcgggctc gaccgggaga tccaggtccg
     2461 gctcgacccg gagcggctcc gctcctaccg gctgaccgtc tccgaggtcc tcacgcgcgt
     2521 ccgcgaggag aaccaggaca tcgccgcggg gcgcctccag tcgacgagcc gggacgtcgt
     2581 gtcgaagacg aaggggaagt tcacctcgcc cgccgacatc gcggccgtcc gcctgccgct
     2641 gcccggcggt ggcgacgtcg ccctctccga tgtcgcggag gtgcgcgaca cgcacctcga
     2701 cgatcgggtc ttcgcccgtc tcgacggcaa gccggccgtc caggtcgcga tctccaagca
     2761 gcccgacgcg aacacggtcc aggtcgtcga cggctgcaac cgggtcctcg agcggctgaa
     2821 gcgcgacggc ttcttccccc ccgacgtgat cacccaggtc acgcaggacc aggccttctt
     2881 cgtccgcgcc tcggtgagcg gcgtcggcga ggccgcggcg ctgggcgggg gcctggcgat
     2941 gctcgtcgtc ttcctgttcc tccggtccgt ccggcggacg ctcatcatcg ggacggcgat
     3001 cccgatctcg atcctcggct gcgtggcgct gatggggacc tccgggctga cgctcaacat
     3061 catgtcgctc ggcggcctcg cgctcggcat cgggatgctc gtcgacaacg ccatcgtcat
     3121 gctcgagaac atcgaccggc accagcggga gcaccccgac ccggtcgagg cggcgcacgt
     3181 cggcgccggc gaggtcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncccg
     3301 cctcgggaac gagttcctcc cgcaggtgga cgacgggcgc ttccgcgtct cgatcgagct
     3361 gccggtcggc acgcccgtcg acgtgacgaa ccgggccacg ctcgcggcgg agaaggtcgt
     3421 ccgcgagctt cccgccgtga ggcacgtctt cgcggcggcc ggcggcggca tctgggggcg
     3481 cggcatgtgg gtccggcccc gtttcgcgag catggaggtc gaggtcgagc cgcggaggag
     3541 ccgcgggatg acggcctccg cctggctgca gcgcgcccag gccgagctct ccgcgctccc
     3601 cgagctggcc gacgcgcgcg tgcgcgtcca gccgccgcgc atccgcggcc tgcgcacctc
     3661 gacggggacc gaggacctcg aggtgaaggt cttcggcgac gacctcccga cgctccagcg
     3721 cctcggcgag gaggtcgagg cgcggctcgg gaagctctcg ggactctcga acgtcgagac
     3781 gagcctcaag gagacggcgc ccgagctgcg cgtcgtcgtc gaccggcggc gcgccgccga
     3841 cctcggcctc gacgtcggcg aggtcggccg gacggtgagg acggccgtcg gcggctcggt
     3901 cgccacgcgc ctgacggaag gggaccggga gttcgacgtg agggtgaggt tcgacccggc
     3961 gaaggtgcgg gacgcctccg agatggcgtc gctcccgctc ttcccgcgga gcggcgcggc
     4021 gctgcgcctg cgcgacgtgg ccgacgtccg cgaggccgcg gccccgcaga cgatccagcg
     4081 cgagaaccag aaccggctcg tcaccgtcgg agcctcggtc ctcccgcagg tctggtcgat
     4141 cagcgaggcg actgcggagg cgcgaaaggc gctcgccgac ctgaagctgc cggacggcta
     4201 cacggttcgc ttcggcggcc agcaggaggc ggtcgacgag aaccgacgcg tcctcgtcac
     4261 ggtcgtgctc ctggcggtct tcctcgtctt cgcggtcatg gccgtccagt acgagtcgct
     4321 cgtcaacccg ctcgtcatca tggcggcgat cccgctcgcc ctcgtcggcg tcgtcctcgc
     4381 gctgacgctg acggggctcc cgatgtcggc gccggtcatg ctcggcgtca tcctcctcgc
//
LOCUS       DAOTEZ010000369         4438 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_14353_length_4438_cov_6.078029, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000369 DAOTEZ010000000
VERSION     DAOTEZ010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14353_length_4438_cov_6.078029"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..667)
                     /locus_tag="P5164_16005"
     CDS             complement(161..667)
                     /locus_tag="P5164_16005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45442.1"
                     /translation="MNAETKVLYDIRTEPAPVGGTIVSGLGGVLVGVVLLAVVWRHRQ
                     RAARTFAFLWILGWGALSAFTVYGISSRHRDARGWLESRSVEVVEGKVDQLSPATSDQ
                     KGIETFRIGDRLFRIEDGQTKTPGLNRSSVRGGPIRSRATVRLTVHDGTILRVEELAP
                     PPAEPSAP"
     gene            complement(776..2293)
                     /locus_tag="P5164_16010"
     CDS             complement(776..2293)
                     /locus_tag="P5164_16010"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HRY45443.1"
                     /translation="MQTRTKVLSFTALGLAAVVFGMILAGSLDFTRTASADSAAPTTA
                     PAAASAPAHIASASLPSFADIADRAMPAVVSIRSTDIIRPDQNRRGIERFFGDTPFHP
                     FLPDRRQGSGEPEEEQQQSGGTGFLIEADGFVLTNNHVIDGAEKVEVTVGETEIFKAK
                     IVGRDPATDLALLKIEGDRPFPTLPLGDSDRIRVGEWVMAIGDPLQFEKTVTVGVVSG
                     KGRAPRLSEATRSFENLIQTDAAINFGNSGGPLVNAAGEVIGINTAISRFAQNIGFAV
                     PINVARRLIPQLKEGKVVRGYLGVSIGPIDADLQAAMGLPSRDGALVQSVEKGMPADK
                     AGLRHEDVIVRVDQTPVRNNRDLIDYVSSKRPGTKVTLTFLRGGKEMTAVATLGTRAE
                     EGETAEPEPAEDEHREKIGITVEELTPASRRSYSIGSEIEGVVLTHVKNVSAAADAGL
                     REGDVIQEVNGRPVGTVVTFRAELKKAGDARFARFYVHRPGPRPANFVVAVRLKD"
     gene            complement(2394..3611)
                     /locus_tag="P5164_16015"
     CDS             complement(2394..3611)
                     /locus_tag="P5164_16015"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRY45444.1"
                     /translation="MKTSFPGLLGLDPSRPGDQTPVPWPPPGGLDPVHDPFARPAAEE
                     DEAPVPLLALDLEEAVAAEAAPSRWSFTRDRAVFLSVLLHALFGLFVLLVPPAERPAD
                     PDELPPDLLGLNKLWAAPPQNPPIPVHFYPAPGPKASEPGPNPRLSDLDRKAGGGDPR
                     LPKADTPKAVAGPGIRDLAPGRRGQGRGPEDEARAAAEAARLAAAATAVERGAGGGAP
                     NVSEDGKGAPAAKGLAGLSASPLRQIGPDQAARSGAAPARQGSPGGGGDEGGGWESEG
                     GFVDPGPLSFDTKGYDWGAYAAEMIRRIKRNWEIPKLAHYGMKGRLVVRFYLLRDGRV
                     EGLRLLVSSGVPPFDNASMQAIRSSNPFRPLPADFPNPREGVTITFLYNIRPEDEGKP
                     GAPAEPPRPLLPR"
     gene            3804..4286
                     /gene="rimP"
                     /locus_tag="P5164_16020"
     CDS             3804..4286
                     /gene="rimP"
                     /locus_tag="P5164_16020"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HRY45445.1"
                     /translation="MTTLTRPELPIRAETLGRIREALAEKGLELCHVAWVPGARKGRL
                     TLTIDRPEGVTLDDCETASRLSDPILEGDETIPGSWLLEVESPGLDRPLWTLDDCRRF
                     AGRRVRVSTEVPVDGAKRMKGPLEAVEGESLVVMDEDRKRRYTVRFGDVKVVRLIPEF
                     "
BASE COUNT          633 a         1594 c         1553 g          658 t
ORIGIN      
        1 tactacgaga gggtcgtgcg cgacgagggc ggtcgcgtcg tccgggcgaa ccggcacgac
       61 ttcgtcccgg tctcctccgc gaccccggcc ccggccgcgc cgcactcgcc cgcgccgctc
      121 gacccggctc tcgcgtcttc gtagcgcggc ccgagcccct tcagggcgcg gagggctcgg
      181 ccggcggagg cgccagctcc tcgacgcgga ggatcgtgcc gtcgtggacc gtcagcctca
      241 cagtcgcccg gctccggatc ggcccgcccc gcaccgagct ccggttcagc ccgggcgtct
      301 tcgtctgccc gtcctcgatc cggaagagcc ggtcgccgat ccggaacgtc tcgatcccct
      361 tctggtcgga cgtcgccggc gagagctggt cgacctttcc ttcgacgacc tcgacggatc
      421 ggctctcgag ccagccgcgc gcgtcccgat gccgggacga gatcccatac acggtgaagg
      481 ccgagagcgc gccccagccg aggatccaga ggaacgcgaa cgttcgcgcg gcgcgctgcc
      541 tgtgacgcca gacgacggcg aggaggacga ctccgacgag aaccccgccc aggccgctca
      601 cgatcgtccc gccgacgggc gccggctccg tccggatgtc gtagagcacc ttcgtctcgg
      661 cgttcaccgc cccggatgat gcctcgaaac ccgcgtcggc gttccgccgg agacgcgaac
      721 gccccgcgcc ggacggcgcg gggcgttcgg accggccccc ggacggggct gcggctcagt
      781 ccttgaggcg gaccgcgacg acgaagttcg ccggccgcgg cccggggcga tggacgtaga
      841 agcgcgcgaa ccgcgcgtcc ccggccttct tcagctcggc ccggaaggtg acgaccgtcc
      901 cgacgggccg gccgttcacc tcctgaatga cgtcgccttc gcgcaagcct gcgtccgcgg
      961 cggccgagac gttcttcacg tgggtcagga cgaccccctc gatctcgctg ccgatcgagt
     1021 agctcctccg ggacgccggc gtcagctcct cgaccgtaat cccgatcttc tcgcggtgct
     1081 cgtcctcggc cggctccggc tcggccgtct cgccctcctc cgcgcgcgtc ccgagcgtgg
     1141 cgacggcggt catctccttg cctccccgca ggaaggtcag ggtcaccttc gtcccggggc
     1201 gcttgctcga gacgtagtcg atcaggtcgc ggttgttccg caccggcgtc tggtcgacgc
     1261 ggacgatgac gtcctcgtgc cgaagccccg ccttgtcggc cggcattccc ttctcgaccg
     1321 actggacgag cgctccgtcg cggctcggga ggcccatcgc ggcctggagg tcggcgtcga
     1381 tggggccgat cgagactccg aggtagccgc ggacgacctt tccttccttc agctgcggga
     1441 tgaggcggcg cgcgacgttg atcggaacgg cgaagccgat gttctgggcg aaacgggaga
     1501 tcgcggtgtt gatgccgatc acctcgcccg cggcgttgac gagcgggccg ccggagttcc
     1561 cgaagttgat cgccgcgtcg gtctggatca ggttctcgaa ggaacgggtc gcttccgaga
     1621 ggcgcggggc gcgccccttc ccggacacga cgccgaccgt gaccgtcttc tcgaactgga
     1681 gcgggtcgcc gatcgccatg acccactcgc cgacgcggat ccggtcggag tcgccgagcg
     1741 ggagcgtcgg gaaggggcgg tctccttcga tcttcaggag cgcgaggtcg gtggcggggt
     1801 cgcgaccgac gatcttcgcc ttgaagatct cggtctcccc gaccgtcacc tcgaccttct
     1861 cggccccgtc gatgacgtgg ttgttcgtca ggacgaagcc gtcggcctcg atgaggaacc
     1921 cggtcccgcc cgactgctgc tgctcctcct cgggctcgcc ggagccctgg cggcggtccg
     1981 gaaggaacgg gtggaacggc gtgtcgccaa agaagcgctc gatgccccgg cggttctggt
     2041 ccggcctgat gatgtcggtg gatcggatcg agacgacggc gggcatcgcg cggtcggcga
     2101 tgtccgcgaa ggacgggagg ctggcggagg cgatgtgcgc cggcgcgctc gccgcggcgg
     2161 gcgcagtcgt cggagccgca gagtcggccg atgcggtgcg ggtgaaatcg aggcttcccg
     2221 cgaggatcat cccgaagacg accgccgcga ggccgagcgc ggtgaacgag aggaccttgg
     2281 tccgggtctg catggtctct ttctccatcg aaggccttca aagcaaggcc catgcccggc
     2341 aaagcagcgt cgttcaggcc ggggtcgaac tcgagattgt cgcccgccgg aagctatctg
     2401 ggaaggaggg ggcgtggcgg ctccgccggc gcaccaggct tcccctcgtc ttccgggcgg
     2461 atgttgtaca ggaacgtgat cgtcaccccc tcccggggat tcgggaagtc ggcgggaaga
     2521 ggccggaacg ggttcgagct ccggatcgcc tgcatggagg cgttgtcgaa cgggggaact
     2581 ccggacgaca cgaggaggcg cagcccttcg acccgcccgt cccgcagcag gtagaaacgg
     2641 accacgagcc gccccttcat tccgtagtgc gcgagcttcg gaatctccca gttccgcttg
     2701 atcctgcgga tcatctccgc cgcgtacgct ccccagtcgt aacccttcgt gtcgaaggag
     2761 aggggtccgg ggtcgacgaa gcccccttcc gactcccagc cgcctccctc gtctccgcct
     2821 ccgcccggcg agccctgccg cgcgggcgcg gctccggagc gcgcggcctg atccggcccg
     2881 atctgccgga gagggctcgc cgagagtccc gcgagcccct tcgcggcggg cgctcccttc
     2941 ccgtcctcgg agacgttcgg cgccccgcct cccgcccccc gctccaccgc ggtcgccgcg
     3001 gccgcgaggc gggcggcctc ggcggccgcg cgcgcctcgt cttccggccc tcgtccttgc
     3061 ccgcgccggc ccggagcgag atcgcggatt ccgggaccgg cgaccgcctt cggcgtatcc
     3121 gctttcggga ggcgcggatc gcccccaccc gccttccgat cgaggtcgga aagccgcggg
     3181 ttcggccccg gctccgaagc cttcggcccg ggcgccgggt agaaatggac ggggatcggc
     3241 gggttctgag ggggcgcggc ccagagcttg ttgagcccca gaagatccgg cggcagctcg
     3301 tcggggtccg cgggccgctc ggcgggcggg accaggagga cgaacaggcc gaacagcgcg
     3361 tgcaggagaa cggaaaggaa gaccgccctg tcgcgggtaa agctccagcg agacggagcc
     3421 gcctccgccg cgacggcttc ctcgaggtcg agcgccagca gcggtacggg ggcctcgtcc
     3481 tcctcggccg ccgggcgggc aaagggatcg tgaaccggat cgagcccgcc cggcggcggc
     3541 cagggaacgg gcgtctggtc cccgggtcgg gacggatcga ggccgagcag gcctggaaag
     3601 gaggtcttca attctcgttc aggcgacacg aaagcgcgct cggcccggcg tatgggaact
     3661 ccgtgccacg cccggcggct ctccggtgct ccatcttacc cggcaccggc ttgcccggaa
     3721 ggcggcgcag tggtatgaat caccttctgg gagctgactt tcaagggctt gagggtgggc
     3781 ttcgacgccc gcccttttca ttggtgacga cgctgacacg acccgaactt ccgattcgcg
     3841 ccgagaccct cgggcgcata cgagaggccc tcgccgagaa gggcctcgag ctttgtcacg
     3901 tcgcatgggt cccgggcgcc cggaaggggc gcctcaccct gaccatcgac cgccccgaag
     3961 gcgtcacgct cgacgactgc gaaaccgcct cgcgcctctc ggacccgatc ctcgagggcg
     4021 acgagacgat cccgggctcc tggctcctcg aggtcgagtc tccgggcctc gatcggccgc
     4081 tctggacgct cgacgactgc cggcgattcg ccggccgccg cgttcgcgtc tcgaccgagg
     4141 tgccggtcga cggcgcgaag cggatgaagg ggcctctcga agccgtcgaa ggcgagtcgc
     4201 tcgtcgtcat ggacgaagac cgaaagcgcc gttacactgt tcggttcggc gacgtcaagg
     4261 tcgtccgcct gatcccagag ttctgaaccc gagaccgacc ggacaccgcc gcgcatggag
     4321 aaacccgaca cgatgagctc gaccgaggcc ccgacaaccg cctcccggcc caggacggcc
     4381 cgctccgcga aggccgccaa ggcggccaag gccccgcccc cgacaaaggc gatcgaga
//
LOCUS       DAOTEZ010000370         4427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_14409_length_4427_cov_5.779048, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000370 DAOTEZ010000000
VERSION     DAOTEZ010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14409_length_4427_cov_5.779048"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            358..2703
                     /locus_tag="P5164_16025"
     CDS             358..2703
                     /locus_tag="P5164_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45446.1"
                     /translation="MSANDLYRRSVGSAGVFLRNLASVRFLVAAEKTSRGLLLGAIAA
                     GLLSAGDAAAIHLVCKSPSATVPCRIWLVPAEATRPVVELAGGRKASVEPGRYFLRAE
                     SPQGVLPAPAAVVVVPGESEKGEPDEVLLRPGARVEVPDGLVGEAGAVHLLSLDSGAI
                     ETLFLAQRRFTLEPAGRAIAVGLAGPGLVAGVTAPFEVPGSGAVKVPPLQNPAPGTGH
                     VVVSWAYPEGSARPDDGLTPLLRSGERTLEASESRFDRDRLHWAVYYDVPAGRWSAVV
                     RSRRWRSTPREIEVIDRRTSFPDPVPLLRKAELTLGLERSGDLADEPFDVTVYRVPAD
                     ECEPARRRGHLSPALDEERAEVLDRQRSVRGEARVPGVDPGCVAVAFECSGRRTHVVE
                     EVGDEDLAIVGKLAPIHVTGTLRWSGKGGPGSLRFTHLNDGTVEDEAEADRDGAYRAT
                     VWQAGFFSVRIAPADGGPPATKRLRVPPGTESVEADFDLPSETFRFRVVDATSNEPVP
                     GAVACCIPGLEEELARPAADADGRLAFRIPRPERERTAEDPVRLPLRFSAEGYEPSNL
                     EIDPLRPPAGEEVVRLRKEDDRSRFFALLPDGSPAAGASVFLAPAGVASDDVARTAVV
                     ACDRAGECRLGKVYPESQSLAIVHAAAGITTATLGEISRTGTVRLLPRAGGFELRVVR
                     GPQSLGRLLSLSVVLNGREVPVAALQTALLIGGAPVGGGAQVAPGGPPEALLRATLLP
                     PGGVRVGILARVPGSSGPAEIVSPARDVWVPGDAPAEIQLP"
     gene            2940..3635
                     /locus_tag="P5164_16030"
     CDS             2940..3635
                     /locus_tag="P5164_16030"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY45447.1"
                     /translation="MSSDRAVASLQDVSKVFEKGSRTVKALDGVSFTVNRGEAVGLLG
                     PNGSGKTTTVRILLGLRKATSGRASVSAGKVGFVLDLPALYPQLTLARNLEFYADLLQ
                     VPRSAIDRRLGDVGLRGEAESRVGELSLGMKKRAEIARALLTDPDLLLLDEPTSGLDP
                     EAQAALRAVLRAEVERGVSILMTSHDLYNVQKVCGRVLVLRHGRLVADEPAANLDPAG
                     LEALYLHAMGRPA"
     gene            3632..4333
                     /locus_tag="P5164_16035"
     CDS             3632..4333
                     /locus_tag="P5164_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45448.1"
                     /translation="MIGILVRKELRLLTATSAVALVLDVLLVGLGLSAVMTLGRLQAL
                     AGVPQGGDRFALFAVLSFGLASMFLSLTGPTALLERTTGILDNQLGMVASPRPLLVAK
                     AVVLVGLAWASLVLWTGVGVAWAAATGQEFVPAGAWGRSLLVVAVLYPAAVALLSLCH
                     VFVGYMVPKLAPIANIGLFAVTFVLFSNLVNLSGSLSAWSRWDLAATTGVVLAVVAGL
                     VWLMGIVSKERMADP"
BASE COUNT          529 a         1582 c         1626 g          690 t
ORIGIN      
        1 agctctccct cgaggtgcgc gagatccgct gcgagaacgg cgagatccgc gtcgcgttcg
       61 gcccgagccg cctccgcctc gaccgcctcg tcgcctccct cgaccaggac ccgttcgacc
      121 tcgagtgagg cggatcgggg aggctcgccg cgagcctccc cgcggcggtc gccggtcttt
      181 cccctcgaaa gggagctccg tgagcgggct ggtggggttc ggggcgggtt gacgccaatt
      241 tcgggggggg gggacgattc gcttcgtcgg atccgttgac gttcccctcc tgaaagtagt
      301 ctggaggcca ttgtgacggc gacgccgctg ccggaatctc tcgaccccat tttcctcatg
      361 tccgcgaacg acctttaccg ccgatccgtg ggaagcgctg gagtcttcct ccgaaacctc
      421 gcgtccgtcc gcttcctcgt cgcagccgaa aagacgtccc gcgggctgct cctcggagcg
      481 atcgcggccg ggcttctctc cgcgggcgat gccgctgcga tccatctcgt ctgcaagtca
      541 ccctcggcga cggttccgtg ccgcatctgg ctggtgccgg cggaggcgac gcggccggtg
      601 gtcgagctgg cgggcgggcg gaaggcgtcg gtcgagcccg ggcggtactt cctccgggcg
      661 gagtctccgc agggtgtcct gccggccccc gcggccgtcg tcgtcgttcc cggagagagc
      721 gaaaaggggg agccggacga ggtcctcctg cgccccggcg cgagggtcga ggtcccggac
      781 gggctcgtgg gcgaagccgg cgccgtgcac ctcctctcgc tcgactcggg cgccatcgag
      841 acgttgttcc tggcgcagcg acggttcacg ctcgagcctg cggggcgggc catcgccgtc
      901 ggcctcgccg gccccggtct cgtcgccggc gtcaccgctc cgttcgaggt tcccggctcg
      961 ggtgcggtaa aggtcccgcc gctgcagaac ccggcgcccg gcaccgggca cgtcgtcgtc
     1021 tcctgggcgt accccgaggg ctcggcccgg cccgacgacg ggctcacgcc cctccttcgg
     1081 agcggagaac ggaccctgga ggcgtcggag tcccgattcg accgggaccg ccttcactgg
     1141 gccgtctact acgacgtccc cgcgggtcgc tggagcgcgg tcgtgcgctc ccgcaggtgg
     1201 cgttcgacgc cccgggagat cgaggtcatc gaccgccgga cctcgttccc cgatcccgtc
     1261 ccgctccttc ggaaggccga gctgacgctc gggctcgagc ggagcggaga cctcgcggac
     1321 gagccgttcg acgtcaccgt ctaccgcgtg ccggcggacg agtgcgagcc cgcgcggcgg
     1381 cggggccatc tctcgccagc cctcgacgag gaacgcgccg aggtcctcga ccgccagagg
     1441 tccgtccgcg gggaagcccg cgtcccgggc gtcgacccgg gctgtgtggc ggtcgcgttc
     1501 gagtgcagcg ggcgccggac ccacgtcgtc gaggaggtcg gggacgaaga cctcgcgatc
     1561 gtcgggaagc tcgcgccgat ccacgtcacg gggacgttga gatggtccgg gaagggaggg
     1621 ccggggagcc tgcgcttcac gcacctgaac gacgggacgg tggaggacga ggcggaggcg
     1681 gaccgggacg gcgcctaccg ggcgacggtc tggcaggcag ggttcttctc ggtgaggatc
     1741 gcgcctgcgg acgggggacc gccggcgacg aagcggctgc gcgtgcctcc cggcaccgag
     1801 tcggtcgagg cggacttcga cctgccttcg gagaccttcc gcttccgcgt cgtcgacgcc
     1861 acctcgaacg agcccgtgcc gggagccgtg gcgtgctgca tccccggcct cgaggaggag
     1921 ctggcgcggc ctgcggcgga cgcggacggg cgtctcgcgt tccggattcc gcgcccggag
     1981 cgcgagcgaa ccgccgaaga ccccgtccgt ctgccgctcc gcttctctgc ggaggggtac
     2041 gagccgagca acctcgagat cgaccccctc cggccccccg cgggagagga ggtcgtgcgg
     2101 ctccggaagg aggacgaccg ctcccggttc ttcgccctgc tgccggacgg gtcgccggcc
     2161 gcgggggcct cggtcttcct cgcgcccgcc ggcgtcgcct cggacgacgt cgcgcgaacg
     2221 gcggtcgtgg cctgtgaccg cgcgggggag tgccggctcg ggaaggtcta tccggagagc
     2281 cagtcgctcg cgatcgtcca cgccgcggcg gggatcacga ccgcgacgct cggcgagatc
     2341 tcacgaacgg ggacggtccg ccttctcccg cgggccggtg gcttcgagct tcgcgtcgtt
     2401 cggggcccgc agtcgctggg ccggctcctt tccctctcgg tcgtcctcaa cggccgggaa
     2461 gtccccgtgg cggcgcttca gacggctctc ctgatcgggg gcgcgcccgt cggcggcgga
     2521 gcgcaggtgg cgcccggcgg tcctcccgaa gcgctcctcc gggcgacgct tcttccgccg
     2581 gggggcgttc gcgtgggaat cctggcgcgc gttcccggga gcagcggacc ggcggagatc
     2641 gtctcgccgg cacgggacgt ctgggttccg ggggacgccc ccgccgagat ccagctcccc
     2701 tgagagcccg gccggtctga cccgccggag cggcgcgacg gttcgcgccg ctccggcggg
     2761 gaagtcgctc aggtgagatg ggcctccgtg agtgtcccga ctgaatccgg acccgcacgg
     2821 gccgcgtccg aactcgtcaa tggaccagag cacatctcgt cgttagtctc cccgcgcatg
     2881 tccgcacgaa ttcaacgagc ctcgacgcgc gagagggcgg gagcttcgga gacgttccgt
     2941 tgagctccga tcgagcggtg gcctccctcc aggacgtctc gaaggtcttc gagaagggga
     3001 gccgcacggt gaaggcgctc gacggggtga gcttcaccgt gaaccggggg gaggccgtcg
     3061 ggttgctcgg cccgaacggc tcggggaaga cgacgacggt tcgcatcctg ctcgggctgc
     3121 ggaaagccac gtcggggcgg gcctcggtct ccgcagggaa agtgggtttc gtcctcgacc
     3181 tccccgccct ctacccgcag ctgacgctcg cccggaacct cgagttctat gccgacctgc
     3241 tgcaggtgcc gaggagcgcg atcgaccggc ggctgggcga cgtgggcctc cggggagagg
     3301 cggagtcgcg ggtcggggag ctctcgctcg gcatgaagaa gcgggccgag atcgcgcgag
     3361 ctctcctgac cgatccggac ctcctcctcc tcgacgagcc gacctccggg ctcgatccgg
     3421 aggcccaggc cgcgctccgg gccgtcctcc gtgccgaggt cgagcggggc gtctcgatcc
     3481 tcatgacgag ccacgacctc tacaacgtcc agaaggtctg tggccgcgtc ctcgtccttc
     3541 ggcacggccg gctggtggcc gacgagccgg ccgcgaacct cgatccggcg ggactcgagg
     3601 cgctctacct ccacgcgatg gggcggcccg cgtgatcggc atcctcgtcc ggaaggagct
     3661 ccggctcctc acggcgacgt ccgccgtggc gctcgtcctg gacgtcctcc tggtcggcct
     3721 cggcctctcc gcggtgatga cgctgggccg gcttcaggcg ctcgcgggcg tgccgcaggg
     3781 gggcgaccgg ttcgcgctct tcgccgtcct ctccttcggc ctcgcgagca tgttcctgag
     3841 cctgaccggg ccgacggcgc tgctggaacg gacgacgggc atcctcgaca accagctcgg
     3901 gatggtggcc tcgccgaggc cgctcctcgt cgcgaaggcc gtcgtcctcg tcggcctggc
     3961 ctgggcgagc ctcgtgctct ggacgggggt cggcgtcgcc tgggccgcgg cgacggggca
     4021 ggagttcgtc ccggccgggg cctgggggcg gagcctcctc gtcgtggcgg tcctctaccc
     4081 cgcggcggtc gcgctcctct ccctctgcca cgtcttcgtc ggctacatgg tcccgaagct
     4141 ggctcccatc gcgaacatcg ggctcttcgc cgtcacgttc gtcctcttct cgaacctcgt
     4201 gaacctctcc gggagcctct cggcgtggtc gagatgggac ctcgccgcga cgacgggcgt
     4261 cgtcctcgcc gtcgtcgcgg gactcgtctg gctgatgggg atcgtctcga aggagcggat
     4321 ggcggacccg tagagagcgg gagcgcaggg gccgttgtgc ggacggcggc gctcggccgc
     4381 cgtccgtccc gtcctcagtc gatcacgacc ctcacgtggg cgtcgtc
//
LOCUS       DAOTEZ010000371         4418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_14449_length_4418_cov_7.752464, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000371 DAOTEZ010000000
VERSION     DAOTEZ010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14449_length_4418_cov_7.752464"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..1284
                     /locus_tag="P5164_16040"
     CDS             79..1284
                     /locus_tag="P5164_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005036312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRY45449.1"
                     /translation="MAQFVWKGKTRQGVMTAGVLSADSKDLAIADLRRRQIEVASVKE
                     RGKEFALPKMGAGKVAPKRLATFTRMFSVMIDAGLPLVQCLEILGTQQDDKAFQRIIL
                     AVRQDVEAGSSLADALKKHPRAFDDLFVNMVAAGEAGGILDTILRRLANYIEKAVKLA
                     GQVKTALIYPVSVLTVAALVVAVILWKVIPTFAALFAGLGAQLPLPTRVVIALSNFLA
                     DYFLFIIAAIAAVVFALKRYYKTYKGRRVIDGVLLKLPVLGEILKKIAVARFCRTFAT
                     LTSSGVPILDGLEITAKTSGNAIIEDGIMAVRKSVETGRTIAEPLAETKVFPPMVVQM
                     IGVGEQTGALDAMLSKIAEFYEEEVDRAVEGLMKLMEPIMIAMLGTIIGTIVVAMYMP
                     MFDLISKVG"
     gene            1376..3058
                     /locus_tag="P5164_16045"
     CDS             1376..3058
                     /locus_tag="P5164_16045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY45450.1"
                     /translation="MSEPARALPRPAPDVLRGLLVTRVVVVSTLLLSALLIQLTFSLW
                     LPLAPIYYLAAGAYTTSLLSLATLRTLDPGANAVLQLLGDLVVVTGLVWVSGGPDSSF
                     TFLYLAVVLSGTLLFGRGGGLVTAGLASVFYAVLLQLMQSGAVSAPRSGELPPRIWTR
                     GQLAGNVVSNVGAFAATALLVAAASERLRDARLLAERRREEVARLQALHSSVLSSMSS
                     GVVTADLSGTITFANPPAGELLHRRPEELVGRPLFETGLVWGEILDLERPPGNEVLRY
                     EGNRTALGAGTYFGVTVTPLRDGDGRVTGRILIFQNLTELKKLEGEVRLKEKLAAVGE
                     LAASIAHEIRNPLASISGSVQVLSGTTPPGSSERRLMEIAVQESHRLSAILEDFLKYV
                     KPRDQAIETVDAAGALRDVVTLLTHSDELSGRHAVTLTLEPESFALQADPGQLRQVFW
                     NLLRNAVAAMPDGGTLAVAARTDDEGSWTATFSDDGRGMSRDEQDRLFTPFAHATPGG
                     TGLGLAIVYRIVEEHGGRIRVKSAPGEGTTITLTLPAGGPGARTAEREGART"
     gene            3055..>4418
                     /locus_tag="P5164_16050"
     CDS             3055..>4418
                     /locus_tag="P5164_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRY45451.1"
                     /translation="MTGAPHRAAGPRILVVDDEPGITGMLSIVFEREGYEVRTAGSCA
                     EGLRLVDELHPDLVLTDLRMPDGTGFDVLRKTRETDAETPVVMITAYTSTKTAIEALK
                     AGAYDYVSKPFDVDELKHVVGKALERKRLAEENQELRSRVGDSPALGPLVGVSRKMRA
                     VFDLIDRVGRTTSTVLITGDSGTGKGLIARAIHRSSARAGRPFVSINCGALPENLLES
                     ELFGHEKGAFTGAVREKKGLFKEAEGGTLFLDEIGETSLAMQVKLLQVLQERVVRRVG
                     GNVEESIDVRIICATNKDLAEKVADRSFREDFYYRIAVIPLQIPPLRERREDIPLLVR
                     HFLKKVTAEQRIPEKKISAEAIRLLEAYPWPGNVRELENLIERTVALEPGPVITTASL
                     PESFLRPAGVPAEALQTPFDLPTGSFDLEAYLEWIGKRLMQQALERANGVRKEAAKLV
                     GMSER"
BASE COUNT          646 a         1634 c         1495 g          643 t
ORIGIN      
        1 gtggggcagg cggcgccggc ggcgccggcc gcgggagccg ccgcccggcg gccctgacgc
       61 gcaccgggag gctgagagat ggcccagttc gtctggaagg ggaagacccg ccagggcgtc
      121 atgaccgcgg gcgtcctgtc cgcggactcg aaggacctcg cgatcgcgga cctccgccgc
      181 cggcagatcg aggtcgcctc cgtgaaggag cgcggcaagg agttcgcgct cccgaagatg
      241 ggcgcgggca aggtcgcgcc gaagcggctc gcgaccttca cccggatgtt ctccgtcatg
      301 atcgacgccg gcctgcccct cgtgcagtgc ctcgagatcc tcggcacgca gcaggacgac
      361 aaggcgttcc agaggatcat cctcgccgtg cggcaggacg tggaggcggg ctcctccctc
      421 gccgacgccc tgaagaagca cccgcgcgcc ttcgacgacc tcttcgtgaa catggtcgcc
      481 gccggagagg cgggcggcat cctcgacacg atcctccgcc gcctcgcgaa ctacatcgag
      541 aaggcggtca agctggcggg ccaggtcaag accgccctca tctacccggt ctccgtcctc
      601 accgtggcgg ctctcgtcgt cgcggtcatc ctctggaagg tcatcccgac gttcgcggcg
      661 ctcttcgcgg ggctcggcgc gcagctcccg ctccccaccc gggtcgtcat cgcgctgtcg
      721 aacttcctcg cggactactt cctcttcatc atcgcggcga tcgccgcggt cgtcttcgcg
      781 ctgaagcgct actacaagac ctacaagggg cgccgcgtca tcgacggggt cctgctgaag
      841 ctcccggtcc tcggcgagat cctcaagaag atcgccgtcg cccgcttctg ccggacgttc
      901 gccacgctga cctcctcggg cgtcccgatc ctcgacggcc tcgagatcac ggccaagact
      961 tccgggaacg cgatcatcga ggacggcatc atggccgtcc ggaagtccgt cgagacgggc
     1021 cggacgatcg ccgagccgct tgccgagacg aaggtcttcc cgccgatggt cgtccagatg
     1081 atcggcgtcg gcgagcagac gggcgcgctc gacgcgatgc tctcgaagat cgccgagttc
     1141 tacgaggagg aggtcgaccg ggcggtcgag ggcctcatga agctcatgga gccgatcatg
     1201 atcgccatgc tcggcacgat catcggcacg atcgtcgtcg cgatgtacat gccgatgttc
     1261 gacctgatct ccaaggtcgg ctgaagccct ctcctcaccc gctcccccga cgggcgccct
     1321 ccggggcgcc cgtcgcgcgt ccggcccccc gcgcggcccg ctacaatcgc ccgacgtgag
     1381 cgaacccgcg cgcgccctcc cgcgcccggc cccggacgtc ctccggggcc ttctcgtcac
     1441 gcgggtggtc gtcgtctcga cgctcctcct ctccgccctc ctgatccagc tgacgttcag
     1501 cctctggctt ccgctcgcgc cgatctacta cctcgccgcg ggcgcctaca cgacgtccct
     1561 cctctccctc gcgaccctcc ggacgctcga ccccggggcg aacgccgtcc tccagctcct
     1621 cggcgacctc gtcgtcgtga ccggcctcgt ctgggtctcc ggcggccccg actcgagctt
     1681 cacgttcctc tacctcgcgg tcgtcctcag cgggacgctg ctgttcgggc gcggcggcgg
     1741 gctcgtgacg gccggcctcg cctcggtctt ctacgccgtc ctcctccagc tgatgcagag
     1801 cggcgccgtg tcggcccccc gcagcggaga gctcccgccc cgcatctgga cgcgcgggca
     1861 gctcgcgggg aacgtcgtct cgaacgtcgg agctttcgcc gcgacggcgc tcctcgtcgc
     1921 ggccgcctcg gagcggctgc gcgacgcccg gctcctcgcc gagcgccggc gggaggaggt
     1981 cgcgcggctc caggccctcc actcgtcggt cctctcctcc atgtcctccg gcgtcgtgac
     2041 cgccgacctc tccggcacga tcacgttcgc gaaccctccg gccggagagc tcctgcaccg
     2101 gcgcccggag gagctcgtcg gacgcccact cttcgagacg ggcctcgtct ggggggagat
     2161 cctcgacctc gagcgccccc ccgggaacga ggtcctccgg tacgagggga accgtacggc
     2221 gctcggagcc ggcacctact tcggggtgac cgtcacgccg ctccgggacg gggacggccg
     2281 cgtgaccggg aggatcctca tcttccagaa cctcaccgag ctgaagaagc tcgaggggga
     2341 ggtccgcctc aaggagaagc tggccgccgt cggcgagctc gcggcgtcga tcgcgcacga
     2401 gatccggaac cccctggcct cgatctccgg ctcggtccag gtcctctcgg gaacgacgcc
     2461 gcccggctcc tcggagcgtc ggctgatgga gatcgcggtc caggagtcgc accggctctc
     2521 cgccatcctc gaggacttcc tgaagtacgt gaagccccgc gaccaggcga tcgagacggt
     2581 cgacgcggcg ggcgccctgc gcgacgtcgt cacgctcctg acgcacagcg acgagctctc
     2641 cgggcggcac gccgtgacgc tgacgctcga gcccgagtcg ttcgcgctcc aggccgaccc
     2701 cgggcagctc cgccaggtct tctggaacct cctccggaac gccgtcgccg cgatgccgga
     2761 cggcgggacg ctcgcggtgg ccgcccggac cgacgacgag gggtcctgga ccgccacctt
     2821 ctccgacgac ggacgaggca tgtcgcggga cgagcaggac cgcctcttca cgccgttcgc
     2881 ccacgcgacg ccgggcggga ccgggctcgg gctcgccatc gtctaccgta tcgtcgagga
     2941 gcacggaggg aggatccggg tgaagagcgc cccgggagaa gggaccacga tcacgttgac
     3001 gctccccgcc gggggcccgg gtgcccgaac cgccgagaga gagggggccc ggacgtgacg
     3061 ggcgctccgc atcgcgccgc cggcccccgc atcctcgtcg tcgacgacga gcccggcatt
     3121 accgggatgc tctcgatcgt cttcgagcgg gaggggtacg aggtccggac cgccggctcg
     3181 tgcgccgagg ggctgcgcct cgtcgacgag ctccaccccg acctcgtcct gacggacctg
     3241 cggatgccgg acggcaccgg gttcgacgtc ctgcggaaga cgcgggagac ggacgccgag
     3301 accccggtcg tgatgatcac ggcgtacacg tccacgaaga ccgccatcga ggcgctcaag
     3361 gcgggcgcct acgactacgt ctcgaagccg ttcgacgtcg acgagctcaa gcacgtcgtc
     3421 gggaaggcgc tcgagcgcaa gcgcctcgcc gaggagaacc aggagcttcg ttcccgcgtc
     3481 ggggacagcc cggcgctcgg cccgctcgtc ggcgtgtcgc gaaagatgcg cgccgtcttc
     3541 gacctgatcg accgcgtcgg gcggacgacg tcgaccgtcc tgatcaccgg cgattccggg
     3601 accggcaagg gcctcatcgc ccgggcgatc caccgcagct cggcccgggc cggacgcccg
     3661 ttcgtctcga tcaactgcgg cgccctcccg gagaacctcc tcgagtcgga gctgttcggg
     3721 cacgagaagg gagcgttcac gggcgccgtg cgggagaaga aggggctctt caaggaggcc
     3781 gagggcggga cgctctttct cgacgagatc ggggagacct ccctcgccat gcaggtgaag
     3841 ctcctgcagg tcctccagga gcgggtcgtg cgccgcgtcg gcgggaacgt cgaggagtcg
     3901 atcgacgtcc ggatcatctg cgcgacgaac aaggaccttg ccgagaaggt cgccgaccgc
     3961 tcgttccgag aggacttcta ctaccggatc gccgtcatcc cgctccagat cccgccgctg
     4021 cgcgagcgcc gggaggacat cccgctcctc gtgcgccact tcctgaagaa ggtgacggcc
     4081 gagcagcgga tccccgagaa gaagatctcg gcggaagcga tccggctcct cgaggcgtac
     4141 ccctggccgg ggaacgtgcg cgagctcgag aacctgatcg agaggacggt cgccctcgag
     4201 ccggggccgg tcatcacgac ggcctcgctc ccggagagct tcctccggcc cgccggcgtg
     4261 ccggcggagg cgctccagac cccgttcgac ctgccgacgg ggagcttcga cctcgaggcc
     4321 tacctcgagt ggatcggcaa gcgcctcatg cagcaggcgc tcgagcgcgc gaacggagtc
     4381 cggaaggaag ccgcgaagct cgtcgggatg tcggagcg
//
LOCUS       DAOTEZ010000372         4412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_14472_length_4412_cov_5.986688, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000372 DAOTEZ010000000
VERSION     DAOTEZ010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14472_length_4412_cov_5.986688"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2463)
                     /locus_tag="P5164_16055"
     CDS             complement(<1..2463)
                     /locus_tag="P5164_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45452.1"
                     /translation="MGQAAAGTIAFTPDPVQVRVGESSQLVTATIQYVGTPPGGSQTL
                     LFNGGSGLSGAVTSPSPVTFTPVAGGPTTVSFQLVASANAYPGPFEASVATTGATGLM
                     NYEILPFSVAPASVLVGAGNTVTGLTATVSYPDFTPTGPQQLTFSGLPAGATPVPSPV
                     TFTVSGTSAVVGFGIATSPATTPGSYTVTVGTTPTATGTDTFELLVAPGSSIAVAPST
                     TTVLPGGTTPGLTATVAFGPPVPTGPQQLVFTGLPPGATPVPSPVSFSVFQIPTVSVP
                     FSVATSAATPPGSYVVTVGTSPASAGTASFELVVAPPGRLELSLASPSIDVCPGGPAV
                     ENTAAVTPAEGFEGSAEVTFPLLPAELVVTPLSIPVEALPPAQTVSFAVSARPDATPG
                     QKVVVVRVASPFGVAAVATFAVNVAVPGFTPVLAPAALTLTAGGGTGKAVVSLAPSAC
                     PTTAETILVTPSGLPAGVTVTPGSAVLFGPTYPPVEFTFVAASAAPAGAATATFAFEP
                     SGGAAKSLPFPITVQRPGRLAIVAERPTMELCAGGGEAPNTLTVNGLDGYTGTPTVTF
                     PGLPAGITATPATLEVPSVPPARVLSFTLRAAAGLAAGDRAVTVKVADPNGVEATTTV
                     VARVLPPDFAPSVAPSNLVLNAGGAAGTITASLAPGSCPPSTDVTITPSGLPPGVVVT
                     PSSALVSPPDFAPALFTFLAGAGAEPGPSTITFTWVPNGATPQATTATISVCGAPAAP
                     GSPAIRPAGNPEGPVTATDFLDLSWAPPASGNPATRYDWRINGGAWTSAAGTFAAAPP
                     RGAVDPVQLFVRAFACDPEKGPG"
     gene            2661..3590
                     /locus_tag="P5164_16060"
     CDS             2661..3590
                     /locus_tag="P5164_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HRY45453.1"
                     /translation="MARTRRGARALSPLLAVVLFASGCSVRKVAVHSLANALAAGGDS
                     YATDDDPELVAAAIPFGLKTMEALLAETPKHDGLLLAASSGFTQYAFAFVQAEADFVE
                     EKDLARATALRARSRRLYARALRYGLRGLEARHPGFEAGLRDEARRAATLAAATKSDV
                     PLLYWTGAAWGAAISLSKEDAELTADQGLAEALERRALELDEAFGRGSVHDFFISFEG
                     GRPAAAGGSAERARRHLERAIALSDGKRAAPYVSFAESVSVGAQNRTEFEELLKKALA
                     IDPDSVPELRLANTVSQRRAAWLLARADELFLE"
     gene            3597..>4412
                     /gene="dctP"
                     /locus_tag="P5164_16065"
     CDS             3597..>4412
                     /gene="dctP"
                     /locus_tag="P5164_16065"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRY45454.1"
                     /translation="MTARRPQTARRRLLLTALALLLAASFPARAQVLVKMATLAPDGS
                     SYHLILKEMAERWKTATQGRVTVRLYPGSVAGDDADVVRKMRLGSLNAGLLTSSGVSA
                     IDPSVMALQVPMLYASYDEVDHVLAVYGPKLEASIAAKGFVVLNWADAGWVRFFTKSP
                     VRTPSDLKPLKLFAWGNDTDALEIWKGAGFNPVPLPSTEISTALQTGLVSALPTPPTA
                     AVLLQWYQHAKNMTDVKWGLLLGALLVNKATWDKISPEDQKAVRAASAEAGARL"
BASE COUNT          642 a         1589 c         1615 g          566 t
ORIGIN      
        1 ccccggcccc ttctcggggt cgcaggcgaa cgcgcgcacg aagagctgga ccgggtcgac
       61 cgcgcctcgc ggcggtgcgg ccgcgaaggt cccggcggcg gaggtccagg cccctccatt
      121 gatccgccag tcgtagcggg tcgccgggtt gcccgaggcc ggcggcgccc aggagaggtc
      181 gaggaaatcg gtggccgtaa ccggtccctc gggatttccg gcaggccgga tcgcgggcga
      241 gccgggagcc gcgggcgcgc cacagacgga gatcgtcgcc gtcgtcgcct gcggcgtcgc
      301 gccgttcggc acccaggtga aggtgatcgt cgaggggccg ggctccgcgc cggcgcctgc
      361 gaggaacgtg aagagggccg gtgcgaagtc cggcggggaa accagcgccg agctcggcgt
      421 gacgacgacg cccggcggaa ggcccgacgg cgtgatcgtg acgtcggtcg acggcggaca
      481 gcttcccggc gccagggacg ccgtgatcgt cccggcggcg ccaccggcgt tcaggacgag
      541 attcgacggg gcgacggagg gggcgaaatc gggcgggaga acgcgggcca cgaccgtcgt
      601 cgtcgcctcc actccattgg gatcggcgac tttcacggtc accgcgcggt cgcctgccgc
      661 gaggcccgcc gcggcgcgga gcgtgaagga gaggacgcgc gccggcggca cgctcgggac
      721 ctcgagcgtc gccggggtcg ccgtgatccc ggccgggagc cctgggaagg tcacggtcgg
      781 cgtccccgtg tagccgtcga ggccgttgac cgtcagcgtg ttcggcgcct cgccgccgcc
      841 cgcgcagagc tccatcgtcg gccgctcggc gacgatcgcg aggcgccccg ggcgctggac
      901 cgtgatcggg aacggaaggc tcttcgccgc gccgcccgag ggctcgaacg cgaacgtcgc
      961 cgtggccgcg ccggccgggg ccgcggacgc cgccacgaac gtgaactcga cgggaggata
     1021 ggtggggccg aaaaggaccg ccgatcccgg cgtcacggtg acgccggccg ggagccccga
     1081 gggcgtcacg aggatcgtct ccgcggtcgt cgggcaggcc gaaggggcga gcgacacgac
     1141 ggccttcccg gtcccgcccc cggcggtcag cgtcagggcc gcgggcgcga ggaccggcgt
     1201 gaagccgggc accgcgacgt ttacggcgaa cgtcgcgacg gcggcgacgc cgaacgggct
     1261 ggccacccgg acgacgacga ccttctgccc tggcgtggcg tccggccggg ccgaaacggc
     1321 gaacgaaacc gtctgcgccg gaggcagggc ttcgacggga atcgagagcg gggtgacgac
     1381 cagctcggcg ggcaggagcg ggaacgtcac ctccgcgctc ccttcgaatc cctcggcggg
     1441 ggtgaccgcc gccgtgttct cgacggcggg gccgcccggg cagacgtcga tcgagggcga
     1501 cgcgagcgag agctcgaggc gtcccggggg agccacgacg agctcgaacg aggcggtccc
     1561 ggcgctcgcc gggctcgtgc cgacggtcac gacgtagctc ccgggcggag tcgccgcgct
     1621 cgtcgcgacg gagaacggaa cggacaccgt cgggatctgg aagacggaga acgagaccgg
     1681 cgagggcacc ggggtcgcgc ccggagggag accagtgaag acgagctgct gcgggccggt
     1741 cggcaccgga ggcccgaagg cgacggtcgc cgtcagcccc ggcgtcgtgc cgccggggag
     1801 gacggtcgtc gtgctcggcg cgaccgcgat ggagctcccc ggcgcgacga gaagctcgaa
     1861 cgtatcggtc cccgtcgccg tcggcgtcgt tccgacggtg accgtgtagc tgccgggagt
     1921 cgtcgccggg ctcgtcgcga tgccgaatcc gacgaccgcg ctcgtccccg aaaccgtgaa
     1981 cgtgacgggc gaagggacgg gcgtcgcacc cgccggcagg ccggagaacg tgagctgctg
     2041 tgggcccgtc ggcgtgaaat cggggtagga aacggtcgcc gtgagccccg tgaccgtgtt
     2101 tcccgccccg acgaggacgg aggccggggc gacggagaac ggcaggatct cgtagttcat
     2161 cagaccggtg gccccggtcg tcgcgacgct cgcctcgaag ggtcccggat acgcgttggc
     2221 cgacgcgacg agctggaacg agaccgtcgt cggccccccc gccacgggcg tgaacgtgac
     2281 cggcgatggg ctggtcacgg cgccggagag tccgctgccg ccgttgaaga gcagcgtctg
     2341 cgagcccccc ggcggcgttc cgacgtactg gatggtcgcc gtgacgagct gcgagctctc
     2401 cccgacccgg acctggaccg ggtccggcgt gaaggcgatc gtcccggctg ccgcctgacc
     2461 caagatcggc cgcccgtaca ggagagcaag gatgcagagg aaagaaccgc ggagagccgc
     2521 tctctgcgac atcgtcgctc ctttcgcctc cggcatcctc gcggatgctc cgggcgtctc
     2581 gaatccaggg gccggtggca cctactctag cagctacgga gtggacggga acccctccac
     2641 gcggtttatt ctcgtgacgg atggcacgca cccgtcgcgg ggcccgagcc ctctcgcccc
     2701 tcctggccgt cgttctcttc gcctcgggct gctccgtccg aaaggtggcc gtccattcgc
     2761 tcgccaacgc cctcgcggcc ggcggcgaca gctacgcgac ggacgacgat cccgagctcg
     2821 tcgctgccgc gatccccttc ggcctgaaga cgatggaggc tctcctcgcc gagacgccga
     2881 agcacgacgg gctccttctc gcggcctcga gcggattcac gcagtacgcc ttcgcgttcg
     2941 tccaggccga ggccgacttc gtcgaggaga aggacctcgc ccgggcgacg gcgctccgcg
     3001 cccgctcccg gaggctctac gcccgtgcgc tccgctatgg cctccggggc ctcgaagccc
     3061 gccaccccgg tttcgaggcg ggccttcgcg acgaggcccg gcgcgccgca acgctcgccg
     3121 cggcgacgaa gagcgacgtc cccctcctct actggacggg ggccgcctgg ggcgcggcga
     3181 tctccctctc gaaagaagac gccgagctga cggcggacca ggggctcgcc gaggcgctcg
     3241 agcgccgcgc gctcgagctc gacgaggcgt tcggcagagg atcggtccac gacttcttca
     3301 tctcgttcga ggggggccgc cccgccgcag ccggaggctc cgccgagcgc gcgcgcaggc
     3361 acctcgagcg cgcgatcgcc ctttccgacg ggaagcgcgc cgctccgtac gtctccttcg
     3421 ccgagtccgt ctccgtcggg gcgcagaacc gcaccgagtt cgaggagctc ctgaagaagg
     3481 cgctcgcgat cgaccccgac tccgtccccg agcttcggct cgccaacacc gtctcgcagc
     3541 gccgggccgc ctggctcctc gcgcgcgccg acgagctctt cctggagtag accgaaatga
     3601 cagctcgacg cccgcagacc gcccgccggc ggctcctcct caccgcgctc gccctcctcc
     3661 tcgcggcctc gttcccggcc cgcgcgcagg tcctcgtgaa gatggcgacg ctcgctcccg
     3721 acggctccag ctaccacctc atcctcaagg agatggccga gaggtggaag acggcgacgc
     3781 aggggcgcgt cacggtccgc ctctaccccg ggagcgtcgc gggcgacgac gcggacgtcg
     3841 tccggaagat gcgcctcgga agcctgaacg ccggcctcct gacgagctcc ggcgtctcgg
     3901 cgatcgaccc gtccgtgatg gcgctgcagg tgccgatgct ctacgcctcc tacgacgagg
     3961 tggaccacgt cctcgccgtc tacggcccga agctcgaggc atcgatcgcc gcgaaggggt
     4021 tcgtcgtcct gaactgggcc gacgccggct gggttcgctt cttcacgaag tcgccggtcc
     4081 ggacgccctc ggacctgaaa ccgctgaagc tcttcgcctg ggggaacgac acggacgccc
     4141 tcgagatctg gaagggggcc ggcttcaacc cggtcccgct tccctcgacg gagatctcca
     4201 cggcgctcca gaccggcctg gtgtcggccc tcccgacacc gcccaccgca gccgtcctcc
     4261 tccagtggta ccagcacgca aagaacatga ccgacgtgaa gtgggggctc ctgctcggcg
     4321 cgttgctcgt gaacaaggcc acctgggaca agatttcgcc tgaggatcag aaggcggtcc
     4381 gcgccgcctc cgccgaggcg ggggcgcgcc tg
//
LOCUS       DAOTEZ010000373         4409 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_14484_length_4409_cov_6.493569, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000373 DAOTEZ010000000
VERSION     DAOTEZ010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14484_length_4409_cov_6.493569"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..1988)
                     /locus_tag="P5164_16070"
     CDS             complement(60..1988)
                     /locus_tag="P5164_16070"
                     /inference="COORDINATES: protein motif:HMM:NF014565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004576 - oligosaccharyl transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STT3 domain-containing protein"
                     /protein_id="HRY45455.1"
                     /translation="MSPARTGRNERVLLATAFIVALGVRLLSAAAATEGGLRLGSPDA
                     YAHLRRANAIARQFPSVTVRDPWLNHPDGGVFIWPPAFDLLGGGISRLAFGADATRDE
                     VARVLAVLPPLLGALHVVPLFLLARRVLSRRRAALAAGAYSVLPVAAVWSQFGHADQH
                     VAEALLLLLLLLALARTRAAGERRASFGRAFAAGTALAAMVLTWQGAVFAGVLSFAWA
                     AFALRPSAAVTFAVSSAGLTALGTLPYARGLELPFTFVSFGWFQPALLLAGASAVAVI
                     AAVRTRGRPGRSVLAGLGLLLLAAAAPVAPDLARGLFGGVRYVVSHQAGVARDEMARG
                     GYLSYPKDWIGLIAESRPLLRPPFLATLRNAVEESSPGIVLLPVALALWVRRRPDRRL
                     LGLFGAALLLMTLSQQRNEYYLAPFAALALAEGLARLAPRGVMRPRPAAIAAASLLVV
                     LPGLPHYGRIVSYAGAPGTDLVETLERLGTRYPPPFDPAGRPLPEPGSAEGVFCPWSA
                     GHFVTALSGYPAAADPFGYGFRRQARLYTTLDDAEAEGILREARCAWLFTTDLRPVLP
                     AYAAAAGRPPTPLAATFAVRVHESASPRPLPFLELAFDSRTAYRAPDGRLVPRFRVFR
                     VRTATEAAEASSRAPDAP"
     gene            2015..3742
                     /locus_tag="P5164_16075"
     CDS             2015..3742
                     /locus_tag="P5164_16075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY45456.1"
                     /translation="MLRPGRRAAAAGAVLLALLGALLVVDHQRLDSATMDEPFHALAG
                     AEYALSGTYWANLEHPPLLKLLAGASAGFAGARPPRLPKPFQYRTAETPQRFLYENAI
                     PPERLLAAVRLPFPLLFAALVLLAATAAGRSAGPLAALGAALLLAFEPTLVAHAGLLH
                     TDVGAALGFLATVLAADAALRRPSFSMWAVTGLLLGLSLAAKFTAVFLVPILLVLALA
                     RMLADRRSAGRLSVAPLAGVALAGAVALPVLLGVYAVSMRRMPAPEAERSVRLFLSGR
                     LAAPATVERVAGLSRLCPPAGHYVAGLVGIAEQNRRGGGINVLRGELSVDGFPSYFPV
                     AFAVKSSLGFLGALALAAALLVVRRVRAGWGALVVLLPPAYVFLSSVSSSYNIGVRHV
                     LPAVPFLAVGAVVAIVRGLPRGAATAALAALGLLQAAETLAVHPHELSFFNRLAGGSA
                     KGSLWLNDSNLDWGQDLVRLARELERRGASDGATVAYFGGASPLHHLPRARLFDAAST
                     PLTPGLYAVSAYLLAAGPELMALRGDAARAAGYDRLRRTLAERGEPAGRVGYSILLYR
                     IPGGEAAPP"
     gene            3739..>4409
                     /locus_tag="P5164_16080"
     CDS             3739..>4409
                     /locus_tag="P5164_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY45457.1"
                     /translation="MKLSIIMPAYNERKTLREIVGRVLAVDLGGIEKELVIVDDGSTD
                     GTRDILRELDGKDGVRAVFQPRNLGKGAAVWTGMRTSTGDLVVIQDADLEYDPREYSV
                     LLRPILDGEADVVYGSRFLGHPGGHRVLYFWHTVGNRMLTLMSNMFTNMNLTDMETCY
                     KAMTRSVVDRLDLQSKRFGIEPEITCKVARMRARVYEVPISYHGRTYEEGKKIGFKDA
                     LQAVWV"
BASE COUNT          588 a         1592 c         1676 g          553 t
ORIGIN      
        1 cgggagggac ggtgcgaccg cacgggccat gcccccgggc gtccccgtgc cgccgcgtct
       61 cagggagcgt cgggcgctcg agaagacgct tccgccgcct ccgtggccgt tctgacccgg
      121 aaaacgcgga agcgcgggac gaggcgcccg tccggagccc gataggcggt cctcgagtcg
      181 aaggcgagct cgaggaacgg gagcggccgg ggcgaggccg actcgtggac gcggacggcg
      241 aacgtcgcgg cgagaggtgt cggggggcgg ccggcggcgg cggcgtaggc cgggaggacg
      301 ggtcgcaggt cggtcgtgaa gagccatgcg caacgcgcct cgcggaggat cccctcggct
      361 tcggcgtcgt cgagagtcgt gtagagacgg gcctgccggc gaaagccgta gccgaacggg
      421 tcggcagccg ccgggtagcc ggagagggcg gtcacgaagt gccccgccga ccaggggcag
      481 aagacccctt ccgcggaacc cggctcgggg agcgggcggc cggccggatc gaacggggga
      541 gggtagcggg tcccgaggcg ctcgagggtc tcgacgaggt cggtcccggg tgcgccggcg
      601 tagctcacga tccggccata gtggggcagc ccgggaagga cgacgaggag cgacgccgcc
      661 gcgatcgcgg ccggccgcgg ccgcatcacc ccgcggggag cgaggcgggc cagcccctcg
      721 gcgagcgcga gcgccgcgaa cggggcgagg tagtactcgt ttcgctgctg cgagagcgtc
      781 atcagcagga gcgccgcgcc gaagaggccc aggagcctgc gatccgggcg tcgccggacc
      841 cagagagcca gggcgacggg gaggaggacg attccggggg aggactcctc caccgcgttc
      901 cggagcgtcg cgaggaaggg cgggcgaagg agcggacgcg actcggcgat gaggccgatc
      961 cagtccttcg ggtaggagag gtagccgccg cgcgccatct cgtcgcgggc gacgccggcc
     1021 tggtggctga cgacgtatcg gaccccgccg aagagccccc gcgcgaggtc gggcgcgacc
     1081 ggcgcggcgg ccgcgaggag gaggaggccg aggccggcga ggacgctccg tcccggcctt
     1141 cctcgggtac ggacggcggc gatcacggcg acggccgacg ctccggcgag gaggagggcg
     1201 ggctggaacc acccgaagga gacgaacgtg aacggcagct cgaggccccg cgcgtacggc
     1261 agcgttccga gggcggtgag gccggcggag gagacggcga acgtgaccgc ggccgacgga
     1321 cgcagcgcga aggcggccca cgcgaacgag aggaccccgg cgaacaccgc cccctgccac
     1381 gtcaggacca tcgcggcgag cgccgtcccg gcggcgaacg cgcgaccgaa cgaagctcga
     1441 cgctctccgg cggcccgggt cctcgcgagg gcgaggagga ggaggaggag gaggagggcc
     1501 tcggcgacgt gctggtcggc gtgcccgaac tggctccaga ccgccgcgac cggcaggacg
     1561 gagtaggcgc ccgcggcgag ggccgcgcgg cgccgcgaga ggacccgacg agcgaggagg
     1621 aagagcggga cgacgtggag cgcgccgagg aggggcggga ggacggccag gacgcgagcc
     1681 acctcgtccc gcgtcgcgtc ggcgccgaag gcgagccggg agatcccccc gccgaggagg
     1741 tcgaaagcgg gcggccagat gaagacgccc ccgtcggggt ggttcaacca cgggtccctc
     1801 acggtgacgg acgggaactg gcgcgcgatc gcgttcgcgc ggcgcaggtg ggcgtaggcg
     1861 tcgggcgagc cgaggcggag gccgccctcc gtcgcggcgg cggcggagag gagccgcacg
     1921 ccgagagcga cgatgaacgc cgtcgcgagg aggacgcgct cgttccggcc ggtccgggcg
     1981 gggctcacct gccgagtctt acacttcgcg cgccatgttg cgtccgggac gtcgggccgc
     2041 cgcggcgggg gccgtcctcc tcgccctcct cggggcgctc ctcgtcgtcg accaccagcg
     2101 actcgacagc gccacgatgg acgagccgtt ccacgcgctc gccggcgccg agtacgccct
     2161 gtcggggacc tactgggcga acctcgagca ccctccgctc ctgaagctcc tcgcaggagc
     2221 gtccgcgggc ttcgccgggg cccggcctcc gcgtctgccg aagcctttcc agtatcggac
     2281 ggcggagacg ccgcagcgct tcctctacga gaacgcgatc ccgccggagc ggctcctcgc
     2341 cgccgtccgc cttccgttcc cgctcctctt cgccgcgctc gtgctcctgg cggcgacagc
     2401 cgcgggacgg tccgccggtc cgctcgcggc cctcggcgcc gcgctcctcc tcgcgttcga
     2461 gccgacgctc gtcgcgcacg cggggctcct ccacaccgac gtcggcgccg cgctcggctt
     2521 cctcgccacc gtcctcgcgg cggacgccgc cctgcgccgg ccgtccttct cgatgtgggc
     2581 cgtgacgggc ctcctcctcg gcctctcgct cgccgcgaag ttcacggcag tcttcctggt
     2641 ccccatcctc ctcgtcctgg ccctcgcccg gatgctcgcg gaccggagga gcgcggggcg
     2701 tctctccgtc gccccgctcg ccggcgtcgc cctcgcgggc gcggtcgccc tcccggtcct
     2761 cctcggcgtc tatgcggtgt cgatgaggag gatgcccgcg cccgaggccg agcggtcggt
     2821 ccgcctgttc ctttcgggac gcctcgcggc cccggcgacg gtggagcggg tcgccggcct
     2881 ctcgcgcctc tgtccgccgg cggggcacta cgtcgcgggc ctcgtcggga tcgccgagca
     2941 gaaccggcgc ggaggcggga tcaacgtcct ccgcggcgag ctgtcggtcg acggcttccc
     3001 gtcgtacttc ccggtcgcct tcgcggtgaa gtcgtcgctc ggcttcctcg gcgcgctcgc
     3061 gctggcggcg gccctcctcg tcgtgcgccg cgtgcgggcc gggtgggggg cgctcgtcgt
     3121 cctgcttccc cccgcgtacg tcttcctctc gagcgtgtcg agcagctaca acatcggggt
     3181 ccggcacgtc ctcccggcgg tcccgttcct cgcggtcggt gcggtcgtgg cgatcgtgag
     3241 gggtcttccg cgcggcgccg cgacggcggc gctcgcggcc ctcgggctcc tccaggcggc
     3301 ggagaccctc gccgtccatc cccacgagct ctccttcttc aaccggctgg ccggagggtc
     3361 ggcgaagggc tccctctggc tgaacgactc gaacctcgac tgggggcagg acctggtccg
     3421 tctcgcgcgc gagctcgagc ggcgcggcgc gagcgacgga gcgaccgtcg cctacttcgg
     3481 gggcgcctcg ccgctccacc acctcccgcg ggcccgcctc ttcgacgccg cgtcgacgcc
     3541 gctcacgccc ggcctctacg cggtctccgc gtacctcctc gccgcggggc ccgagctgat
     3601 ggcgctgcgc ggggacgcgg cccgggccgc gggctacgac cggctccggc ggactctggc
     3661 cgagcggggc gagcccgcgg ggcgcgtggg atactcgatc ctcctgtacc gcattccggg
     3721 gggagaagcc gcgccgccat gaagctctcg atcatcatgc ccgcctacaa cgagcggaag
     3781 acgctccggg agatcgtcgg ccgcgtcctc gcggtcgacc tcggcgggat cgagaaagag
     3841 ctcgtgatcg tcgacgacgg gtcgaccgac ggcacgcgcg acatcctccg cgagctggac
     3901 gggaaggacg gcgtccgggc cgtttttcag ccccgcaacc tcggcaaggg ggcggccgtc
     3961 tggaccggca tgcggacgtc cacgggcgac ctcgtcgtca tccaggacgc cgacctggag
     4021 tacgaccctc gcgagtactc cgtcctcctc cgccccatcc tcgacggcga ggcggacgtc
     4081 gtctacggct cgcgtttcct cggccacccg ggcggccacc gggtcctcta cttctggcac
     4141 accgtgggca accggatgct caccctgatg tccaacatgt tcacgaacat gaacctgacg
     4201 gacatggaga cctgctacaa ggcgatgacg cgctccgtcg tcgaccggct cgacctgcag
     4261 tcgaagcgct tcggaatcga gccggagatc acctgcaagg tggcgcggat gcgggcccgg
     4321 gtctacgagg tgccgatctc gtaccacggc cggacgtacg aggaagggaa gaagatcggc
     4381 ttcaaggacg ccctccaggc cgtctgggt
//
LOCUS       DAOTEZ010000374         4406 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_14502_length_4406_cov_8.063204, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000374 DAOTEZ010000000
VERSION     DAOTEZ010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14502_length_4406_cov_8.063204"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="P5164_16085"
     CDS             complement(<1..1109)
                     /locus_tag="P5164_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRY45458.1"
                     /translation="MRNAARFAPAFLAFLLASDALPALAATASTKAVDRTGKAEPSFE
                     SRFAGMRFRNIGPFRGGRVTAVSGVRGQRNVHFHGATGGGVWKTTDAGASWEPVSDGF
                     FRTGSVGAVAVAESDPNVVWAGMGEAPIRGNVSHGDGVWRSTDAGRSWKHLGLAATQQ
                     ISALRVHPKDPDTAWVAAQGKLWGPSEERGVYRTRDGGKTWARVLFVDAATGASDLAL
                     DPTNPRVLYAGMWQVVRRPWELVSGGPGSGLYRSDDGGDTWTRLTKGLPEGTWGKVGV
                     SASGARPGRVFAMVEAEKGGLFRSEDWGESWTRVNEENDLRQRAWYYTGVYADPRSAD
                     TVWVSNVQLWRSLDGGKSFTAVPPPHGAHHDLWSDP"
     gene            1240..1410
                     /locus_tag="P5164_16090"
     CDS             1240..1410
                     /locus_tag="P5164_16090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45459.1"
                     /translation="MNGLSTRPRTGEELDRRIRMEWAVRQVQRGFKDVQQAALAARVP
                     VTHLASVVGADA"
     gene            complement(1514..4216)
                     /locus_tag="P5164_16095"
     CDS             complement(1514..4216)
                     /locus_tag="P5164_16095"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRY45460.1"
                     /translation="MTCSPFRPRLARLLLASALAVPMAASAAAPAPKGLPPLLDRELF
                     FGNPEIGGAQLSPDGKWTAFLKPWKETRNVWVKKTGEPYDKARLVTAEPKRPVAGFFW
                     SRDSKYILFVKDNEGDENFNVWAVDPAAKAEAGKDAPPARNLSDAKGVRAQIYAVPKK
                     DPDTVFVGLNDRDAAWHDVYKVKISTGERTLLKKNTEKISGWVFDLEGQLRLAARIAE
                     NGDTEVLRVDPEGFVKIYSCSVFESCGPVRFHKDGKRVYMESNKGAPDLAALFLFDPA
                     TGKEEPVETDPKGRVDFGNAIFSEATDELAATTYEDEKTRIYFRDKAWEADYRLLEKK
                     LPGREVIPAGSTADDQLWMVTAYSDVDPGTRYLFDRKTKKLSVEYVSRERLPREHMAP
                     MTPISYPSSDGLMIPAYLTVPKGVQAKGLPLVVFPHGGPWARDSWGFNNYAQWFANRG
                     YAVLAPNFRGSTGYGKKFLNAGNKEWGLKMQDDITWGVKHLVKEGIADPKRVAIMGGS
                     YGGYATLAGLTFTPDLYAAGVSLVGPSNLLTLLETIPPYWEAIRIIFHERMGNPNTPE
                     GKKLLEAASPLNSAAKIVKPLLVIQGANDPRVKKAESDQIVIALRDRGFPVEYICAPD
                     EGHGFARPVNNMAAMAASEAFLAKHLGGRFQEGGTPEVVARLKEITVDPKTVTLAKKV
                     DAAAVGAPKPVAEPVPGTYAYAGTISAGGQSMQVSATRVIKADGETWVVSDTAKMPMG
                     EATDVTTLAKGTLAPVKRSIRQGPVTIDLAFADGKATGTMAMGAEPKPISADLGGPLF
                     ADGNATYDSIACLPLAAGYAATYRNFDVQKQKATLRQLKVAGTEEVKVAAGTFSAWKI
                     ELTSAEGEPGATTLWVDSASRKVVKTSTTLPQMGGAVVTSELQP"
BASE COUNT          597 a         1532 c         1584 g          693 t
ORIGIN      
        1 gggtcgctcc agaggtcgtg gtgggcgccg tgcgggggcg ggacggcggt gaagctcttc
       61 cccccgtcga gcgagcgcca gagctggacg ttcgacaccc agaccgtgtc ggcgctccgc
      121 gggtcggcgt agacgcccgt gtagtaccag gcgcgctgcc gcaggtcgtt ctcctcgttc
      181 accctcgtcc agctctcgcc ccagtcctcg gagcggaaga ggcccccctt ctccgcctcg
      241 accatcgcga agacgcgtcc cggcctggca ccggaggccg agaccccgac ttttccccac
      301 gtcccctcgg ggagcccctt cgtcagcctc gtccacgtgt cgccgccgtc gtcggagcgg
      361 tagagcccgc tcccgggccc gcccgagacg agctcccacg ggcgcctgac gacctgccac
      421 atcccggcgt agaggacgcg cgggttcgtc gggtcgaggg cgaggtcgct cgccccggtc
      481 gcggcgtcga cgaagaggac gcgcgcccac gtcttcccgc cgtcccgcgt ccggtagacg
      541 ccccgctcct cgctcggccc ccagagcttc ccctgcgcgg cgacccaggc cgtgtcgggg
      601 tccttcgggt ggacgcgcag ggccgagatc tgctgcgtgg ccgccaggcc gaggtgcttc
      661 cagctgcgcc ccgcgtcggt cgagcgccag acgccgtcgc cgtgcgagac gttgcctcgg
      721 atcggcgcct cgcccatccc ggcccagacg acgttcgggt ccgactcggc gaccgcgacc
      781 gcgccgacgg agccggtgcg gaagaacccg tccgacaccg gttcccagct cgcgccggcg
      841 tccgtcgtct tccagacgcc gcccccggtc gccccgtgga agtggacgtt ccgctgcccg
      901 cggaccccgg agaccgccgt gacgcgcccg ccgcggaacg gcccgatgtt ccggaagcgc
      961 attcccgcga agcggctctc gaaggagggc tccgctttcc ccgtcctgtc caccgccttc
     1021 gtgctggccg ttgcggcgag ggccggaagg gcgtccgaag cgagaaggaa cgcgaggaaa
     1081 gccggggcga agcgcgctgc gtttctcacg ggagcctccg gacggagacg ataccggagc
     1141 gcgggagccg cacgggcccg cgcggaatcc cggcgcccgg ggtccggcgt aggagaggtg
     1201 cgcggggcga acgacgctcc cgcgccacgg agacgcgaca tgaacggact ttcgacgagg
     1261 ccccggaccg gagaagagct cgaccgccga atccggatgg agtgggcggt ccggcaggtg
     1321 cagaggggct tcaaggacgt ccagcaggcc gctctcgccg cccgcgtccc agtgactcac
     1381 ctcgcgtccg tcgtcggagc cgacgcctga ggcgtcgcag ccgccgcgca cccgagagag
     1441 ggcgttccgg aaacgaaacg ggggcggcga cccgagcggg tcgccgcccc ctgttgcgat
     1501 ccggggaccg gtcctagggc tgaagctcgg aggtcacgac ggcgccgccc atctgcggca
     1561 gcgtcgtgga ggtcttgacg accttgcggc tcgccgagtc gacccagagc gtcgtcgccc
     1621 cgggctcccc ctcggccgag gtgagctcga tcttccacgc cgagaacgtc cccgcggcca
     1681 ccttcacctc ctcggtgccg gcgaccttca gctgccggag cgtggccttc tgcttctgga
     1741 cgtcgaagtt gcgataggtc gccgcgtacc cggccgcgag cggaaggcac gcgatcgagt
     1801 cgtacgtggc gttcccgtcg gcgaagagcg gcccgccgag gtcggccgag atcggcttcg
     1861 gctcggcccc catcgccatc gtcccggtcg ccttgccgtc ggcgaacgcg aggtcgatcg
     1921 tgaccggacc ctgacggatg ctccgcttga ccggagccag cgtccccttc gcgagcgtcg
     1981 tgacgtcggt cgcctcgccc atcggcatct tcgccgtgtc ggaaacgacc caggtctccc
     2041 cgtccgcctt gatgacgcgc gtcgccgaga cctgcatgct ctggccgccg gccgagatcg
     2101 tcccggcgta ggcgtaggtg ccggggacgg gctccgcgac cggcttcggc gcgccgacgg
     2161 cggccgcgtc gaccttcttc gcaagcgtga ccgtcttcgg gtcgacggtg atctccttca
     2221 gccgggcgac cacctcgggc gtgccgccct cctggaaccg gccgccgagg tgcttcgcga
     2281 ggaacgcctc ggaggcggcc atcgccgcca tgttgttgac ggggcgcgcg aagccgtgac
     2341 cctcgtcggg ggcgcagatg tactccaccg ggaagccgcg gtcgcggagc gcgatgacga
     2401 tctggtccga ctcggccttc ttcacgcggg ggtcgttcgc cccctggatg acgaggagcg
     2461 gcttcacgat cttcgccgcg gagttgagcg gcgaggcggc ctcgaggagc ttcttcccct
     2521 cgggcgtgtt cgggttcccc atccgctcgt ggaagatgat ccggatcgcc tcccagtacg
     2581 gcgggatcgt ctcgaggagc gtcaggaggt tcgacgggcc gacgagcgag acgcccgcgg
     2641 cgtagaggtc gggggtgaag gtgaggccgg cgagggtcgc gtagccgccg taggagccgc
     2701 ccatgatggc gaccctcttc gggtcggcga tcccttcctt cacgaggtgc ttgacgcccc
     2761 acgtgatgtc gtcctgcatc ttcaggcccc actccttgtt gccggcgttc aggaacttct
     2821 tcccgtagcc ggtggagccg cggaagttcg gggcgaggac ggcgtagccg cggttcgcga
     2881 accactgggc gtagttgttg aagccccagg agtcgcgggc ccacgggccg ccgtgcggga
     2941 agacgacgag gggaaggccc ttcgcctgga cgcccttcgg gacggtcagg taggcgggga
     3001 tcatcagccc gtcggacgac gggtagctga tgggcgtcat cggcgccatg tgctcgcgcg
     3061 ggaggcgctc gcgggagacg tactcgacgg agagcttctt cgtcttccgg tcgaagaggt
     3121 agcgcgtccc cgggtcgacg tcggagtagg ccgtcaccat ccagagctgg tcgtcggccg
     3181 tggagccggc ggggatcacc tcgcggccgg ggagcttctt ctcgaggagc cggtagtcgg
     3241 cctcccaggc cttgtcgcgg aagtagatcc gggtcttctc gtcctcgtag gtcgtggcgg
     3301 cgagctcgtc ggtcgcctcg gagaagatcg cgttcccgaa gtcgacgcgc cccttcgggt
     3361 cggtctcgac gggctcttcc ttgccggtcg ccgggtcgaa caggaagagg gcggcgaggt
     3421 ccggcgcgcc cttgttcgac tccatgtaga cccgcttgcc gtccttgtgg aagcggaccg
     3481 ggccgcagct ctcgaagacg ctgcaggagt agatcttgac gaagccttcg gggtcgaccc
     3541 ggagcacctc ggtgtcgccg ttctcggcga tccgcgccgc gagccgaagc tgcccttcga
     3601 ggtcgaagac ccagcccgag atcttctcgg tgttcttctt caggagcgtc cgctcgcccg
     3661 tcgagatctt caccttgtaa acgtcgtgcc aggccgcgtc acggtcgttc aggccgacga
     3721 agacggtgtc ggggtccttc ttcggaacgg cgtagatctg ggcccggacg cccttggcgt
     3781 cggacaggtt ccgcgcgggg ggggcgtcct tgccggcctc cgccttcgcg gccgggtcga
     3841 cggcccagac gttgaagttc tcatcgccct cgttgtcctt gacgaagagg atgtacttcg
     3901 agtcccggct ccagaagaag ccggccaccg ggcgcttcgg ctcggcggtg acgaggcgcg
     3961 ccttgtcgta cggctccccc gtcttcttca cccagacgtt ccgggtctcc ttccagggtt
     4021 tcaggaaggc ggtccacttc ccgtcggggg agagctgggc gccgccgatc tccggattgc
     4081 cgaagaagag ctcccggtcg aggagcggcg ggagcccctt cggggcgggg gcggcggcgg
     4141 acgcggccat cgggacggcg agagccgacg cgagcaggag ccgcgcgagg cgcggccgga
     4201 agggcgaaca ggtcatcgtc gaggcctcct tgagccggtt gagaaggtga agctgcactc
     4261 gatatcgagg tacgtcacgg ggcgcccgaa gttccagtcg gagacggcgt ctccgcgttc
     4321 gtgcccgtta tgagccgggc cgagcgggcc caggtcaggt agctccaggc ccactggacg
     4381 aggacgagga cccggttgcc gaactg
//
LOCUS       DAOTEZ010000375         4364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_14722_length_4364_cov_3.716640, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000375 DAOTEZ010000000
VERSION     DAOTEZ010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14722_length_4364_cov_3.716640"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>4364
                     /locus_tag="P5164_16100"
     CDS             <1..>4364
                     /locus_tag="P5164_16100"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRY45461.1"
                     /translation="APIFVAGVASDANLLEVRVNGQAASLGADGSFQAGPFRPGDGPV
                     TFTAVATDRASRSGTAERFVLVDTTPPEITIRNASTSAPILPGQLSNTPVTLAVSVTD
                     ATTPAQTTHTVTVDGTPYGGGPVSGEGSHLVEVVATDGAGNTATASVGFVVDTIRPVF
                     SALLPEHGSVGRSTPVTVSGRVSADTESVTVSGQPATCAGGAFSLSGVSLSEGPNDLL
                     LVATDRAGNEEKTTLRVVLDTTNPSLTIASPAEGALLGSLSVTVTGAATDANLESVTV
                     EGGAAALQPDGFFSRAGVTLVEGPGTITATARDRAGNTATTSVHVVADSLPPTVRITA
                     PGPGAVLGQSPAAVSGTATDAHLGSVTVNGVAANVDDAGRFTASVPLTEGSNTLTATA
                     SDTLGRTAQASVVVSLDTAAPNVTITSPTDGARFRTTPQTVRGTVDSDLNLEGVTVNG
                     VAATTNGTSFEASVPLIEGTNALTARARKATGKEGTARIDVVFDTTAPTLAASAPADG
                     QSGVALSPVFRLTFSEALDASTVVPSAFVLTAPGASAPSLAATLGGAGSNVVTLVPAA
                     ALAEATEYTLSVAASVADLAGNGLAAPLTIRFTTVDQTPPPAPVLDAVPELLCAEAVT
                     LSGTSEAGAILAVSGGAAGAQGLAGPDGRFSVPVPLAPAATQTLSVTARDASGNVSPA
                     ASVVLTVDCEPPHVVDVTLSSAGLVVALDEPLRPTSVVAGESVRLDTPGGTPIAAAVA
                     HSGGDATLTVTAGGVDLTAVAFTLSLSSGVQDRAGNALVPFVRTFEPQSVATLLLGEA
                     FDDSVGRPLGGATATLLVSAGVETSEPRPYATATAAGGFVLPALPGDALVRVGATGYL
                     DVYRRESVVAGGSGSPVSTTLFDVRLTPIADPASAKPTAGGTLYTATAGSRVATLEAP
                     AASVPPDVVVRLTVRRPQGLPVLAPLGWSVASAARVAISDAAGAPVAPTGAMTLRLPD
                     FYGATTSTALHLARLDPVSLQWQSEGAATLRDGALEAILSAAGDWAILVPDPAPTAPG
                     TLAPGAPLPGVALPAEDPLESASVVADPVDVLPTQASDVSLTVVAPVPVPSGYPVQTL
                     VTEELTLLDGSRLAGPSFLADLSLLRRGDGSLGLSIPVRASEGAGRVALSVGYERFAV
                     KKFPFEVRRGVVVGPTGGTATGSSGWAATLPTGAVSGSTSVALTAIGVSELPASVPQG
                     FDFLAAVRLATGGVAFDLPVVLSKSGAAAPPAGAELLVVAFEERSGFLLTRPVARASY
                     DAASQTLRSSLIDRALFPWPGVRNEGVYAFLAAIDPLAFVAGRVLDVDATALRDTLVS
                     VGGGWTLQAVSETDGTFALAIRAAASTLSAVRTGTGNLGQLTVTPATPGQEITGVELR
                     LAHTAPWVAGVTPAAGSTVLVGSRFTVTFSEAIDPLSLTPASFFLVATVEGQPIVVPS
                     ETLVDPSGLSATLVPAGA"
BASE COUNT          488 a         1781 c         1506 g          589 t
ORIGIN      
        1 ggccccgatc ttcgtagcgg gcgtcgcctc cgacgcgaac ctcctcgagg tcagggtcaa
       61 cggccaggcg gcgagcctgg gcgccgacgg cagcttccag gccgggccgt tccgccccgg
      121 ggacgggccg gtgaccttca cggcggtcgc gacggaccgc gcctcccggt ccggcacggc
      181 ggagcgcttc gtcctcgtcg acacgacgcc gcccgagatc acgatccgga acgcgtcgac
      241 ctcggcgccg atcctcccgg gccagctctc caacacgccg gtgacgcttg ccgtgagcgt
      301 gacggacgcc acgacgccgg cccagacgac gcacaccgtg accgtcgacg ggacgccgta
      361 cggcggcggg ccggtctcgg gcgaggggag ccacctcgtc gaggtcgtcg ccacggacgg
      421 cgccggcaac acggccacgg cctccgtcgg gttcgtggtc gacacgatcc gacccgtctt
      481 ctccgcgctc cttccggaac acgggagcgt cggccggtcg acgccggtca ccgtgtcggg
      541 gcgcgtcagc gccgacaccg agagcgtcac cgtctcgggc cagccggcga cctgcgcggg
      601 cggagccttc tccctgtccg gcgtctccct ctcggaggga ccgaacgacc tcctcctcgt
      661 cgcaacggac cgcgcgggga acgaggagaa gacgacgctc cgggtcgtcc tcgacacgac
      721 gaacccgtcc ctgacgatcg cgtcccctgc ggaaggggcg ctcctcgggt cgctctccgt
      781 caccgtgacg ggcgccgcga ccgacgcgaa cctggagtcc gtcaccgtgg aaggcggcgc
      841 cgccgcgctc cagccggacg gcttcttctc gcgcgcgggc gtcacgctcg tcgagggccc
      901 gggcacgatc accgccacgg cgagggaccg ggccggcaac acggcgacga cgagcgtcca
      961 cgtcgtggcc gactccctgc cccccaccgt ccggatcacg gcgccgggcc ccggggccgt
     1021 cctcggccag agccccgccg ccgtcagcgg aacggcgacc gacgcgcacc tcgggagcgt
     1081 gaccgtgaac ggggtcgccg cgaacgtcga cgacgccggc cgcttcaccg cctccgtccc
     1141 gctcaccgag gggagcaaca ccctcacggc gaccgcgagc gacacgctcg gacggaccgc
     1201 gcaggcgtcc gtcgtcgtct ccctcgacac cgccgccccg aacgtgacga tcacgtctcc
     1261 gaccgacggc gcgcgcttcc ggacgacgcc gcagacggtc cgcgggacgg tcgactcgga
     1321 cctcaacctg gaaggcgtca ccgtcaacgg agtcgccgcg acgacgaacg ggacgtcgtt
     1381 cgaggcgagc gtccccctca tcgagggaac gaacgcgctg acggcccgcg cgcggaaggc
     1441 gaccgggaag gaggggacgg cccgaatcga cgtcgtcttc gacacgacgg ctccgacgct
     1501 ggccgcctcc gccccagccg acggccagtc gggcgtcgcc ctctcgccgg tcttccgcct
     1561 cacgttctcc gaggcgctcg acgcctcgac ggtcgtcccc tcggcgttcg tcctgacggc
     1621 gccgggcgcc tcggccccgt cgctcgcggc gacgctcggc ggcgccggct cgaacgtcgt
     1681 caccctcgtg ccggccgcgg cgctcgccga ggcgacggag tacacgctct cggtcgcggc
     1741 ctcggtcgcg gacctcgccg ggaacggtct cgcggccccg ctcacgatcc gcttcacgac
     1801 cgtcgaccag acgcctccgc cggcgcccgt gctcgacgcc gtcccggagc tcctctgcgc
     1861 ggaagcggtc accctctcgg gaacgtcgga ggcgggagcg atcctcgccg tctcgggcgg
     1921 cgccgccgga gcccagggcc tcgccgggcc ggacggacgc ttcagcgtgc cggtgcccct
     1981 cgcgccggcc gcgacccaga ccctctccgt gacggcccgc gacgcgtcgg ggaacgtctc
     2041 cccggccgcc tcggtcgtcc tcacggtcga ctgcgagccg ccccacgtcg tggacgtcac
     2101 cctgtcgtcc gcgggcctcg tcgtcgcgct cgacgagccg ctccggccga cctcggtcgt
     2161 ggcgggggag tcggtccggc tcgacacgcc cggcggcacc ccgatcgccg ccgccgtcgc
     2221 ccactcgggc ggcgacgcga cgctcacggt cacggcgggc ggcgtcgacc tgacggccgt
     2281 cgcgttcacg ctctccctct cgtcgggtgt ccaggaccgc gcggggaacg ccctcgtccc
     2341 cttcgtccgg acgttcgagc cccagagcgt cgcgacgctc ctcctcggcg aagcgttcga
     2401 cgactccgtc ggccgcccgc tcggcggcgc gacggcgacc ctcctcgtct cggcgggcgt
     2461 cgagacgtcc gagccgcgcc cctacgccac cgcgacggcc gcgggcggct tcgtccttcc
     2521 ggccctgccg ggagacgcgc tcgtgcgggt cggggcgacg ggctacctcg acgtctaccg
     2581 gcgcgagtcg gtcgtggcgg ggggctcggg cagcccggtc tccacgacgc tcttcgacgt
     2641 gaggctgacg ccgatcgccg acccggcctc ggcgaagccg accgccggcg ggacgctcta
     2701 caccgcgacc gcgggatccc gcgtcgcgac gctcgaggct ccggccgcga gcgttccgcc
     2761 cgacgtcgtc gtccgactca cggttcgccg gccgcagggc cttccggtcc tcgctcccct
     2821 cggctggtcg gtcgcctccg cggcgcgggt cgcgatctcc gacgccgccg gcgcccccgt
     2881 ggcgccgacg ggcgcgatga cgctccgcct cccggacttc tacggcgcca cgacctcgac
     2941 ggcgctccac ctcgcccggc tcgatcccgt ctctctccag tggcagagcg agggggcagc
     3001 gacgctccgg gacggcgcgc tcgaggcgat cctctccgcc gcgggcgact gggcgatcct
     3061 cgttcccgac ccggcgccga cggcgcccgg gacgctcgcg ccgggggcgc cgctgccggg
     3121 cgtcgcgctg ccggcggagg acccgctcga gtcggcgtcc gtcgtcgccg acccggtcga
     3181 cgtccttccg acgcaggcct ccgacgtctc cctcaccgtc gtcgctccgg ttccggtgcc
     3241 gtcgggctat ccggtccaga ctctcgtcac cgaggagctg acgctcctcg acggctcccg
     3301 cctcgcggga ccgtcgttcc tggccgacct ctcgctcctc cggcgcgggg acggctcgct
     3361 tggactctcg attcccgtcc gcgcctccga gggcgccggg cgcgtcgctc tctccgtcgg
     3421 ctacgagcgg ttcgccgtga agaagttccc gttcgaggtg aggcgagggg tcgtcgtcgg
     3481 cccgacgggc ggcaccgcga ccggctcctc cggctgggcg gcgacgctcc ccaccggagc
     3541 ggtctcgggc tcgacctcgg tcgcgctgac ggcgatcggc gtctccgagc tgccggcctc
     3601 cgtgccgcag ggattcgact tcctcgcggc ggtgcgtctc gcgacgggcg gcgtcgcttt
     3661 cgacctgccg gtcgtcctct cgaagtccgg agcggccgcg cccccggccg gggcggagct
     3721 gctcgtcgtc gcgttcgagg agcggagcgg cttcctcctg acgcgccccg tggctcgcgc
     3781 ctcgtacgac gcggcgagcc agaccctccg ctcgagcctg atcgaccggg ccctcttccc
     3841 gtggcccggc gtccgcaacg aaggggtcta cgccttcctg gcggcaatcg acccgctcgc
     3901 cttcgtcgcc gggagggtcc tcgacgttga cgcgacggcg cttcgcgaca ccctcgtttc
     3961 cgtcggcggc ggctggactc tccaggccgt cagcgagacc gacgggacct tcgccctcgc
     4021 catccgggcc gcggcctcga ccctctcggc ggtgcggacc gggacgggga acctcggcca
     4081 gctcaccgtc accccggcga ctccgggcca ggagatcacc ggagtcgagc tccggctcgc
     4141 ccacacggcc ccgtgggtcg ccggcgtgac ccccgcggcc ggctcgaccg tcctcgtcgg
     4201 ctcgcgcttc accgtcacct tctccgaggc gatcgacccc ctctcgctca ccccggcgag
     4261 cttcttcctc gtcgccaccg tcgaggggca accgatcgtc gtcccgtccg agacgctcgt
     4321 cgacccgagc gggctctccg cgacgctcgt ccctgccggc gccc
//
LOCUS       DAOTEZ010000376         4356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_14764_length_4356_cov_7.585445, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000376 DAOTEZ010000000
VERSION     DAOTEZ010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14764_length_4356_cov_7.585445"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="P5164_16105"
     CDS             <1..787
                     /locus_tag="P5164_16105"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRY45462.1"
                     /translation="ATDVVFTSLLDWVADKPWELAFDPHRRAFLAATEACFVRNVVTA
                     VPCETRRRRRGRLSVVVAAAQPQGLSSVSWEEEAEAIRAGLAPLVASGLARVRVERAV
                     TAEALHRLVADGPCDVLHFVGHGTWDAERRVGALVLESEGGGVAPVEAPVLRTLLQGR
                     GLALVVLNACQSARGSRGDLARGVAPALVAAGVPAVVANQFSVLDAAALVFARELYRS
                     LALGRALGDAAREARLAVRCDAACGPVDWAVPVVYARDPASAL"
     gene            831..1775
                     /locus_tag="P5164_16110"
     CDS             831..1775
                     /locus_tag="P5164_16110"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY45463.1"
                     /translation="MTIELRPLREDELREADGIYRRAFGTLCGLTDPLAFDGDAERVR
                     CRWASDPGAAIALLEDGALAGTNFVSRWGSVGLLGPLVVDPPRQGRGLAHPLVEEALR
                     LLEQPQVAFRGLYTSVTDPRTVGLYQSHGYWPMSLAVLMQKATAAGREGPAGFEALSR
                     LTPDAQAEALSACRELSDQVFDGLDVTGEVLSVGEQETGDTVLLRDGSSLAGFAVCHA
                     GKGSEAGSGRAAVRFAAVVSGEGGEERLRRLLEAVEAWAASAGAAVVTAGTGSGRVGA
                     WRTLRAAGWLPFQQGVAMHRDRHPGYDREDVFVLDDWR"
     gene            complement(1787..2422)
                     /locus_tag="P5164_16115"
     CDS             complement(1787..2422)
                     /locus_tag="P5164_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HRY45464.1"
                     /translation="MRLPRPLDAVETRVLGALLEKELLTPDVYPMTLPGLLAACNQKT
                     SREPVTELSAAEATAALDRLRELVLVWKVTGGRAEKWEHNLEAKWQAGRPEKAILALL
                     LLRGAQTAAELRARAERMHELASVEAAEALLGAMAAHAEPLVAELPRRPGQKETRWTH
                     LVSGPPPAEASSPRSAAPPRISPMEERLLALEERVAALEAELAALRGSLGG"
     gene            complement(2424..3578)
                     /locus_tag="P5164_16120"
     CDS             complement(2424..3578)
                     /locus_tag="P5164_16120"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRY45465.1"
                     /translation="MSRTTEEQFPALSARIPDAEARALFTWPFLRASEVFDELVDAAS
                     AEVLRRAGAWPVAPGTDPAALVAARGWAPRSLPLVAFVHRKLAAAGHLVASPDGYAHS
                     GTEPGDLSALEAELAAIDADSAVAGEAVRILVEEAGSFLSGERTGEEILFRPDRLPLW
                     FRYFSNRNLLYGVNNSLGALALSRVLPASGARVLEIGGGAGSAAEAALAHLGGRLSRY
                     LFTEVVPTFARRGERAARAAAAEGVAVEAAKLDMTKPWAPQGAAPGAFDAVYSVNCFH
                     VAPDLAAVLAEAREALAPGGSLVVSECVRPLGNDQPIYVELVFDLLESFTRVTLDPLA
                     RPTHGFLTPAAWRESLRRAGFVDVTFLPDAEELARSYPSFFASAVTARKG"
     gene            3614..4267
                     /locus_tag="P5164_16125"
     CDS             3614..4267
                     /locus_tag="P5164_16125"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRY45466.1"
                     /translation="MKIFLVRHGESEGNLGGTLQGCRLDTPLTRRGRRQAEALSVRLA
                     EAEIDDVVASPMARARETAAICAAPHGLAVRLDLELVEFDWGVWTGLPLDDEMDRSVA
                     ELRAKWRSGDVDAAPPRGESPLVAGARAARVLSRLRAAGPAAPLVVAHGRFNRIFAAT
                     LLGRDLARMDEVRQRNGSLSVFEWDGEGAARPLLLDDVSHLGGDVSTPTGKVDWVRA"
BASE COUNT          519 a         1579 c         1714 g          544 t
ORIGIN      
        1 ggcgaccgac gtcgtcttca cgtcgctcct cgactgggtg gccgacaagc cgtgggagct
       61 ggcgttcgac ccgcaccggc gcgcgttcct tgccgcgacc gaggcctgct tcgtgaggaa
      121 cgtcgtcacg gccgtcccgt gcgagacgcg gcggcgaagg cgcggccggc tctcggtcgt
      181 cgtcgcggcc gcgcagccgc agggcctctc gtccgtgtcg tgggaggagg aggccgaagc
      241 gatccgcgcc ggcctcgcgc cgctcgtcgc ctcggggctc gcgcgggtgc gcgtggagcg
      301 ggccgtcacc gccgaggcgc tccaccggct cgtggccgac ggcccgtgcg acgtcctcca
      361 cttcgtcggg cacggcacgt gggacgccga gcgccgggtc ggcgcgctcg tcctcgagtc
      421 ggagggcgga ggggtcgcgc ccgtcgaggc gcccgtcctg aggacgctcc tccagggccg
      481 cgggctcgcc ctcgtcgtcc tgaatgcctg ccagagcgcg cgggggagcc ggggcgacct
      541 cgcccgcggc gtcgcgccgg cgctcgtggc ggccggggtc cccgccgtcg tcgcgaacca
      601 gttcagcgtc ctcgacgctg cggccctcgt cttcgcccgc gagctctacc gctccctcgc
      661 gctcgggcgc gcgctgggcg acgcggcgcg cgaggcgcgc ctggcggtcc gatgcgacgc
      721 cgcgtgcggc ccggtcgact gggcggtccc ggtcgtctac gcgcgcgatc cggcctcggc
      781 gctctgaccg cccccggggg cggtggcaag atggagcagg aggtcgcgtc atgacgatcg
      841 agctccgccc gctccgcgag gacgagctcc gggaggccga cgggatctac cggcgcgcct
      901 tcgggacgct ctgcggcctg accgaccctc tcgccttcga cggagacgcc gagcgcgtgc
      961 ggtgccgctg ggcctccgac ccgggcgccg cgatcgccct cctcgaggac ggcgcgctcg
     1021 ccgggacgaa cttcgtctcc cggtggggaa gcgtcggcct cctcggccct ctcgtggtcg
     1081 acccgccgcg ccaggggcgc ggtctcgcgc atccgctcgt cgaggaggcg ctccggctcc
     1141 tcgaacagcc gcaggtcgca ttccggggcc tctacacctc cgtcacggac ccgaggacgg
     1201 tcggcctcta ccagagccac ggctactggc cgatgagcct cgccgtgctg atgcagaagg
     1261 cgaccgcggc cgggcgggaa gggcccgccg gtttcgaggc gctctcgcgc ctgacccccg
     1321 acgcgcaggc ggaggcgctc tcggcctgcc gggagctctc ggaccaggtc ttcgacggcc
     1381 tcgacgtaac gggggaagtc ctctccgtcg gcgagcagga gacgggggac acggtcctcc
     1441 tccgggacgg gtcgagcctc gcgggcttcg ccgtctgcca cgccgggaag gggagcgagg
     1501 cgggctcggg acgcgcggca gtgcgattcg ccgcggtggt ttcgggcgag ggcggcgagg
     1561 agcgcctgcg gcgcctcctc gaggcggtcg aggcgtgggc ggcctcggcc ggcgcggcgg
     1621 tcgtcaccgc ggggacgggt tcgggacggg tcggagcgtg gcgaacgctc cgggcggccg
     1681 gctggctccc gttccagcaa ggggtggcga tgcaccgcga ccggcacccg ggctacgacc
     1741 gcgaggacgt cttcgtcctc gacgactggc gctaggccgc ggccctctat ccgccgagcg
     1801 agccccggag cgcggcgagc tcggcctcga gcgcggcgac ccgctcctcc agggcgagga
     1861 ggcgttcctc catcggcgag atgcgcgggg gcgcggcgga gcgcggcgag gacgcctctg
     1921 caggcggcgg gcccgagacg aggtgggtcc agcgcgtctc cttctgcccc ggccggcgcg
     1981 ggagctcggc gacgagcggc tcggcgtgcg cggccatcgc tccgaggagc gcctcggcgg
     2041 cctcgacgga ggcgagctcg tgcatccgct cggcgcgcgc gcggagctcg gcggcggtct
     2101 gcgcgccgcg aaggaggagg agggcgagga tcgccttctc cggcctgccg gcctgccact
     2161 tcgcctcgag gttgtgctcc cacttctcgg ctcgcccgcc cgtcaccttc cagacgagga
     2221 cgagctcgcg gagcctgtcg agcgcggcgg tcgcctccgc ggcggagagc tcggtcaccg
     2281 gctcccgcga cgtcttctgg ttgcaggccg cgagcagccc cggaagcgtc atcgggtaga
     2341 cgtcgggggt caggagctcc ttctcgagga gcgccccgag gactcgcgtt tcgacggcgt
     2401 cgagcgggcg cgggagacgc acgtcagccc tttcgcgccg tgacggccga ggcgaagaag
     2461 ctcgggtagc ttcgcgcgag ctcctcggcg tccgggagga acgtcacgtc gacgaagccg
     2521 gcgcgccgga gcgactcgcg ccaggcggcc ggcgtcagga agccgtgcgt cgggcgcgcg
     2581 agagggtcca gcgtgacgcg ggtgaagctc tcgaggaggt cgaagacgag ctcgacgtag
     2641 atcggctggt cgttcccgag cgggcggacg cactcggaga cgacgagtga gccgccgggg
     2701 gcgagcgcct cccgggcctc ggcgaggacg gccgcgaggt cgggcgcgac gtggaagcag
     2761 ttcacggagt agacggcgtc gaacgcgccg ggagcggccc cctgcggcgc ccagggcttc
     2821 gtcatgtcga gcttcgccgc ctcgacggcg acgccctcgg ccgccgcggc gcgcgcggcc
     2881 cgctcgcccc tccgcgcgaa cgtcgggacg acctcggtga agaggtagcg ggagagtcgc
     2941 ccgccgaggt gagccagagc cgcctccgcg gcgctccccg ccccgccgcc gatctcgagg
     3001 acgcgggcgc ccgaggccgg aaggacgcgg gaaagcgcga gggcgccgag cgagttgttg
     3061 acgccgtaga ggaggttccg gttcgagaag tagcggaacc agagcgggag ccggtcgggc
     3121 cggaagagga tctcctcgcc cgttctctcg ccggagagga agctccccgc ctcctccacg
     3181 aggatcctca cggcctcgcc cgcgacggcc gagtcggcgt cgatcgcggc gagctcggcc
     3241 tccagggccg agaggtcgcc cggctcggtg cccgagtgcg cgtagccgtc cggcgaggcg
     3301 acgaggtgcc ccgcggcggc gagcttgcgg tggacgaagg cgacgagcgg caggctcctc
     3361 ggcgcccagc cgcgggcggc gacgagcgcc gccgggtccg tcccgggggc gaccggccag
     3421 gcgccggccc gccgcaggac ctcggccgaa gcggcgtcga cgagctcgtc gaacacttcg
     3481 ctcgcgcgga ggaacggcca ggtgaagagg gcgcgcgcct cggcgtccgg aatccgggcg
     3541 gagagcgccg ggaactgctc ctcggtagtc ctcgacatcg gcgggcatga tacggattcg
     3601 tagaatcgcg cggatgaaga tattcctcgt ccgtcacggc gagagcgaag ggaacctcgg
     3661 cgggacgctc caggggtgcc gcctcgacac cccgctcacg cggcgcggcc gccgccaggc
     3721 cgaagccctc tccgtccgcc tcgccgaggc ggagatcgac gacgtcgtcg cgagcccgat
     3781 ggcccgcgcg cgggagaccg cggcgatctg cgcggcgccg cacggcctcg cggtgaggct
     3841 ggacctcgag ctcgtggagt tcgactgggg cgtctggacc ggcctgccgc tcgacgacga
     3901 gatggaccgg tccgtcgccg agctccgcgc gaagtggcgt tcgggggacg tcgacgcggc
     3961 gccgccgcgc ggcgagagcc cgctcgtcgc gggagcgcgg gcggcgcgcg tcctctcgcg
     4021 ccttcgggcc gccggcccgg ccgcgcccct cgtcgtcgcc cacgggcgct tcaaccggat
     4081 cttcgcggcg accctgctcg gaagagacct cgcccggatg gacgaggtgc gtcagcggaa
     4141 cggctcgctc tccgtcttcg agtgggacgg ggaaggggcc gcgcggccgc tcctcctcga
     4201 cgacgtctcc cacctcggcg gggacgtctc gacgccgacg gggaaggtcg actgggtgcg
     4261 ggcctgagcg ccgcgccgat ccgctgctcc gggttcccaa tcgaggggag gcgccgcagg
     4321 cgccgcgggg aggctcgtgg cgagcctccc cgatcc
//
LOCUS       DAOTEZ010000377         4353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_14779_length_4353_cov_7.418799, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000377 DAOTEZ010000000
VERSION     DAOTEZ010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14779_length_4353_cov_7.418799"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="P5164_16130"
     CDS             complement(<1..207)
                     /locus_tag="P5164_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019980963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45467.1"
                     /translation="MASAAPDAFLPNPPSPGLGGSPRVGALLERAAAGGRLTSSEAVL
                     LLEEAPLLELGAAAAAVRQRLHPGA"
     gene            complement(207..1016)
                     /locus_tag="P5164_16135"
     CDS             complement(207..1016)
                     /locus_tag="P5164_16135"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HRY45468.1"
                     /translation="MTPAIPVSVVDFVNAWPVTWGFLKGAVEGFLPINDVPSVCAERL
                     ARGEVQAGIIPAIEAARIPGVRIVRGVGIAAYERVRSVLLVSRVPFEKVRSVALDTSS
                     RSSAAMARILFADLFGASPEYHRAVPDLPRMLSSADAALLIGDPALTTDVSAYRVLDL
                     AEGWNRLTGLPLVTAVWAVRRDVSPEPFLWSRDYARHRMPEIVDAAMARTGLDRRLLT
                     EYFEMNLHYDLDADDERGLAEFYRRAAAHGLIPSPDLPPFAGVPLVPRSFS"
     gene            complement(1082..2329)
                     /gene="rho"
                     /locus_tag="P5164_16140"
     CDS             complement(1082..2329)
                     /gene="rho"
                     /locus_tag="P5164_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRY45469.1"
                     /translation="MLDIRTLHNTPMPQLLKIAQELEVEGAAALRKQELIFKVLQAQT
                     ERMGLIFSEGVLEVLPDGFGFLRAPEYNYLAGPDDIYVSPSQIRKFGLRTGDTISGQI
                     RPPKEGERYFALIKVEAVNFEHPDVAREKILFDNLTPLYPQERIRLETPNMSSRVLDL
                     IAPVGKGQRGLIVAPPRTGKTMLLQALANSITTNHPEVTLIVLLIDERPEEVTDMQRS
                     VKGEVISSTFDEPATRHVQVAEMVIEKAKRLVEHKRDVVILLDSITRLARAYNTTCPP
                     SGKVLSGGVDANALQKPKRFFGAARNVEEGGSLTIMATALIDTGSRMDDVIFEEFKGT
                     GNMEIHLDRRLVDRRVFPSIDINKSGTRKEELLIEKAELNRIWILRKVLTPLSTVEAM
                     ELLLERLEKSKTNLEFLSSMSAG"
     gene            complement(2440..3147)
                     /gene="yihA"
                     /locus_tag="P5164_16145"
     CDS             complement(2440..3147)
                     /gene="yihA"
                     /locus_tag="P5164_16145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRY45470.1"
                     /translation="MTRKENPVSRTPKAARAVSVRSPLGKSVRLELPAHSAGERPRGL
                     PIPAVAVLGRSNVGKSSLLNALLATKVARVSQTPGRTQALHWYRVDDAFHVVDCPGYG
                     WAKTARASRERFSGLIEELLTGERAPDLALLLVDGRLPPQASDVSMALFLRHAGVPTV
                     VAVTKWDAVKPSQRVRHLRALAAELQDSERPLLPVSSETGENLPTLGRLLKDRLTGRT
                     KDSAAPAAPQGPTQKES"
     gene            complement(3211..>4353)
                     /locus_tag="P5164_16150"
     CDS             complement(3211..>4353)
                     /locus_tag="P5164_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HRY45471.1"
                     /translation="LDEVDKMSMDFRGDPSAALLEVLDPEQNHAFVDHYLDVEYDLSK
                     VMFIATANVIHPIPPALKDRMEIISLSGYTPNEKLAIARQFLVPKQVKENGLTPEQAR
                     FDDSGLYGLIEKYTREAGVRNLEREIASVCRKIARKVVADDSGAAKAGITVTAENLPD
                     FLGKPKHRPAKKGEKAEVGLSTGLAWTEAGGDVLHIETTLMRGKGNLVLTGQLGDVMQ
                     ESARAALSYVRSRAEMYGADADFYEKKDIHVHIPEGAIPKDGPSAGITMATSILSAVT
                     NIACRKDLAMTGEITLRGKVLPVGGIKEKLLAAYRAGITTIVLPRENEKDLDDLPEEI
                     RNVLETHLVDDVDEVVRLALEGSPVAAPPKPVPAEAGTTPPSTLAH"
BASE COUNT          601 a         1457 c         1614 g          681 t
ORIGIN      
        1 ggcgccgggg tggaggcgct gccgcacggc cgcggccgcg gcgccaagct cgaggagcgg
       61 cgcctcctcg aggaggagga ccgcctccga cgacgtgagc cgtccgcccg ccgcggcgcg
      121 ctcgagcagc gcgccgacgc gcggcgagcc tccgaggccg ggggacggag ggttcgggag
      181 gaacgcgtcc ggcgcggcgg aggccatcag gagaacgacc tcgggacgag cggcacgccc
      241 gcgaacggcg ggaggtcggg ggaggggatc aggccgtggg cggcggcgcg ccggtagaac
      301 tccgcgaggc cgcgctcgtc gtcggcgtcg aggtcgtagt ggaggttcat ctcgaaatac
      361 tccgtgagga gcctgcggtc gagccccgtc cgggccatcg cggcgtcgac gatctcgggc
      421 atccggtggc gcgcgtaatc gcgcgaccag aggaacggct cgggggagac gtcccggcgg
      481 acggcccaga ccgccgtcac gagcgggagc cccgtcagcc ggttccaccc ctcggcgagg
      541 tcgaggacgc ggtacgccga gacgtccgtc gtcagcgccg ggtctccgat cagcagcgcg
      601 gcgtcggcgc tcgagagcat ccgcggcagg tcggggacgg cccggtggta ctcgggcgag
      661 gcgccgaaga gatcggcgaa gaggatcctc gccatcgcgg ccgaggagcg cgagctcgtg
      721 tcgagcgcga cgctccgcac cttctcgaac gggacccggg agacgaggag gacgctcctc
      781 accctctcgt acgcggcgat cccgacgccc cggacgatcc gcacgccggg gatccgcgcc
      841 gcctcgatcg ccgggatgat ccccgcctgc acctcacccc gcgcgagccg ctccgcgcag
      901 accgacggca cgtcgttgat cgggaggaag ccctcgaccg cccctttcag gaagccccag
      961 gtgaccggcc aagcgttgac gaagtcgacg acggagacgg ggatcgcggg cgtcatgtcc
     1021 acgccgatct tacgatgccg cccggcgggc cggacgggga cggggacgcc cgccgatcgg
     1081 gctaccccgc cgacatcgac gagaggaact ccaggttcgt cttcgacttc tcgagcctct
     1141 cgaggagcag ctccatcgcc tcgaccgtcg agagcggcgt gaggaccttg cggaggatcc
     1201 agatccggtt gagctccgcc ttctcgatga ggagctcctc cttccgcgtc cccgacttgt
     1261 tgatgtcgat cgacgggaag acgcggcggt cgacgagccg gcgatccagg tggatctcca
     1321 tgttgcccgt ccccttgaac tcctcgaaga tgacgtcgtc catccgcgag ccggtgtcga
     1381 tgagggccgt cgccatgatc gtgagcgagc ccccctcctc gacgttgcgc gccgcgccga
     1441 agaagcgctt cggcttctgg agcgcgttcg cgtcgacgcc gcccgacagg accttccccg
     1501 acggcgggca ggtcgtgttg taggcgcgcg cgaggcgggt gatcgagtcg agcaggatga
     1561 cgacgtcgcg cttgtgctcg acgagccgct tcgccttctc gatgaccatc tcggccacct
     1621 ggacgtggcg cgtcgccggc tcgtcgaacg tcgagctgat gacctcgccc ttcaccgacc
     1681 gctgcatgtc ggtgacctcc tcgggccgct cgtcgatgag gaggacgatg agcgtcacct
     1741 cggggtggtt cgtcgtgatc gagttcgcga gcgcctgcag gagcatcgtc ttgccggtcc
     1801 gaggcggcgc gacgatgaga ccgcgctgcc ccttgccgac cggcgcgatc aggtcgagga
     1861 cgcgcgagga catgttcggc gtctcgagcc ggatccgctc ctgagggtag agcggcgtca
     1921 ggttgtcgaa gaggatcttc tcgcgcgcga cgtcggggtg ctcgaagttg accgcctcga
     1981 ccttgatcag ggcgaagtag cgctcccctt ccttcggcgg gcggatctgc cccgagatcg
     2041 tgtcgcccgt ccggaggccg aacttgcgga tctgggaagg cgagacgtag atgtcgtcgg
     2101 gcccggcgag gtagttgtac tcgggggcgc gcaggaagcc gaagccgtcc ggcagcacct
     2161 cgaggacccc ttccgagaag atcaggccca tccgctcggt ctgcgcctgc aggaccttga
     2221 agatgagctc ctgctttcgg agcgccgcgg ccccctcgac ctccagctcc tgcgcgatct
     2281 tcaggagctg cggcatcggg gtgttgtgca gcgtccggat gtcgagcatc ggcgcgtcct
     2341 cgaagtcggg cgcggccggc tcctcgacgt cgttcgggag gtcgttcggg tcgatcgtcg
     2401 ggctgacgta gtcggttccg gggaccttgc ggcggggcat caggactcct tctgtgtcgg
     2461 accctgcggc gccgcgggcg cggcgctgtc cttggtcctt ccggtgagcc ggtccttgag
     2521 aagacggccg agggtgggca gattttcgcc cgtttcggac gaaacgggca ggagggggcg
     2581 ttcggaatct tgaagctccg cggcgagggc ccggaggtgc cggaccctct gcgacggctt
     2641 cacggcgtcc cacttcgtca cggccacgac ggtcgggacc ccggcgtgcc ggaggaagag
     2701 ggccatcgag acgtccgacg cctgcggggg gagccggccg tcgacgagga gcagcgcgag
     2761 gtcgggcgcc ctctcccccg tcaggagctc ctcgatgagc cccgagaagc gctcgcgcga
     2821 ggcgcgcgcc gtcttcgccc agccgtagcc ggggcagtcg acgacgtgga aggcgtcgtc
     2881 cacccggtac cagtggagcg cctgcgtccg gccgggggtc tgcgagacgc gggccacctt
     2941 cgtggcgagg agggcgttca ggaggctcga cttcccgacg ttcgaccggc cgaggacggc
     3001 gaccgccggg atcggcaggc cgcgcggccg ctcgccggcg ctgtgggccg ggagctccag
     3061 gcggaccgac ttcccgagcg gactcctcac ggagaccgcc cgcgcggcct tgggcgtgcg
     3121 ggagacgggg ttctctttcc gggtcataga cgaagctgaa ggaatcgtgt gccggtccgg
     3181 ccggcccccg tgcgggagcc ggccggcgga tcagtgagcc agggtggagg ggggcgtcgt
     3241 ccccgcctcg gccgggaccg gcttcggcgg ggcggcgacc ggggagcctt cgagggcgag
     3301 gcggaccacc tcgtcgacgt cgtcgacgag gtgcgtctcg aggacgttcc ggatctcctc
     3361 ggggaggtcg tcgaggtcct tctcgttctc gcgcgggagg acgatcgtcg tgatccccgc
     3421 gcggtaggcg gcgaggagct tctccttgat gccgcccacg gggaggacct tcccgcgcag
     3481 ggtgatctcg ccggtcatgg cgaggtcctt gcggcaggcg atgttcgtga cggccgagag
     3541 gatcgaggtc gccatcgtga tcccggccga ggggccgtcc ttcgggatcg ccccctccgg
     3601 gatgtggacg tggatgtcct tcttctcgta gaagtcggcg tcggcgccgt acatctcggc
     3661 ccggctccgc acgtacgaga gcgccgcgcg ggccgactcc tgcatcacgt cgccgagctg
     3721 gcccgtcagg acgaggttcc ccttgccgcg catcagggtc gtctcgatgt ggaggacgtc
     3781 gccgccggcc tcggtccacg cgagcccggt ggagaggccg acctcggcct tctccccctt
     3841 cttcgcgggg cggtgcttcg gcttgccgag gaagtcggga aggttctccg ccgtgacggt
     3901 gatccccgcc ttcgccgctc ccgagtcgtc cgcgacgacc ttccgcgcga tcttccggca
     3961 gaccgaggcg atctcgcgct cgaggttccg cacccccgcc tcgcgggtgt acttctcgat
     4021 gaggccgtag aggccggagt cgtcgaagcg ggcctgctcg ggcgtcagcc cgttctcctt
     4081 gacctgcttc gggacgagga actgccgggc gatcgccagc ttctcgttcg gcgtgtagcc
     4141 cgagaggctg atgatctcca tccggtcctt cagggcgggc gggatcgggt ggatcacgtt
     4201 cgccgtcgcg atgaacatga ccttcgagag gtcgtactcc acgtcgaggt agtggtcgac
     4261 gaacgcgtgg ttctgctcgg ggtcgaggac ctcgaggagc gccgcggacg ggtcgccgcg
     4321 gaagtccatc gacatcttgt cgacctcgtc gag
//
LOCUS       DAOTEZ010000378         4340 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_14843_length_4340_cov_4.922987, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000378 DAOTEZ010000000
VERSION     DAOTEZ010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14843_length_4340_cov_4.922987"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..585)
                     /locus_tag="P5164_16155"
     CDS             complement(109..585)
                     /locus_tag="P5164_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HRY45472.1"
                     /translation="MSDYPDLVDEPEETSAEVPAEEASLPPPPSDADPYFAFLAAEGF
                     RPEVDEDGDVRFRHEGRTLYLFRDGKDPEYFRAALPGAWECDGPEEESRALAAVNSVN
                     RDLKTVKCVLVDGVVWVSVEQFFDPPEAFRPVFTRLLDVIGSAAWQVRERMRASST"
     gene            complement(582..1943)
                     /locus_tag="P5164_16160"
     CDS             complement(582..1943)
                     /locus_tag="P5164_16160"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY45473.1"
                     /translation="MLLARVSPDPVERCTVWSYPVPDGPGWDSAARVRRALLCSAHAY
                     VSADGPASADPLALLLREAGATTVPEAALLVLLLCRGEDDGPLAWGLLEPALGSPAAS
                     LPGPSSPLVAAGLVEVRPSTGSPQLSTCRATADARLRAVPSGMPAPRAARAIGSEVPA
                     SPALVERIEPRLNLDGLVLPLATRRRLTEALAVPAALASLSETGWGVEETLLGAPNVA
                     LLLYGPPGTGKTLCAEAIAGQLGKTLWRLRTDQLLSKFVGETEKRLTEVFAEARRTGD
                     VVLLDEADSFLTTRERAARSWEATMTNVLLQEIERFRGVVVLTTNRDAVLDPALERRL
                     AARLAFPLPGAAERRLLWERHLPPRAPRAADVDLESIAERFALSGSAIRTAALYALAR
                     AASRAGDARVLTRADLEDAARVETARAASHRPAVGFGVATTPSPRLALVAEHRDAARK
                     ERP"
     gene            complement(2006..2506)
                     /locus_tag="P5164_16165"
     CDS             complement(2006..2506)
                     /locus_tag="P5164_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45474.1"
                     /translation="MKRARTAIEPFTSGEECLEAWFLALGPMTGTGGDHPTGIVPIDV
                     RLPHLRALAARSAATRDAGVPTPVLDAIEGLRLDLLDRLLLLALLRDDLDARTNGGLR
                     IATLCDAAGAATWSHQALVCSRLDETGALRRHGLVESDGDPAPGERLYRLAPRWSGSF
                     QRGARR"
     gene            complement(2557..2691)
                     /locus_tag="P5164_16170"
     CDS             complement(2557..2691)
                     /locus_tag="P5164_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45475.1"
                     /translation="MTTLFLVGLVLLLLGSFSESGSKAARRLRRLDPGGRDDFGGRLR
                     "
     gene            2788..3759
                     /locus_tag="P5164_16175"
     CDS             2788..3759
                     /locus_tag="P5164_16175"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HRY45476.1"
                     /translation="MAFKDRPYFRRTLDLDDQLRSGKKVTATTLAARWETSTKTVQRL
                     VDGVRDEYGAPIEWDAHRGTYVYSDPSWHLPWLAMKGADLFAVGVAMKVLQMYEGTPV
                     AKDMRAIYERLSELMPAEVRISPSSFVEKLYIRPQAQRPVDPNVWDAVATGLREKVAI
                     EADYRKPAGGGGWRELEPLHLVLFGTDWFLLARDPEGEAVKTFYLSRMKAVRVGTRRF
                     SPPRGFRPEEHFGDTIGLYVGKPRFRFRVRFGREIAEWITEVRWHEKQKLVRRSDGDV
                     ELELPAGSLLEARRFVLSFGMHAEVLGPEELVEDVKAHVRSLAERYG"
     gene            complement(3892..>4340)
                     /locus_tag="P5164_16180"
     CDS             complement(3892..>4340)
                     /locus_tag="P5164_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diheme cytochrome c-553"
                     /protein_id="HRY45477.1"
                     /translation="LVGIMGCDDCHTPMKMGAKGPEPDFARRLSGHPQAVKVTKPAPI
                     DAKAWIWAGNATNTAFAGPWGISYAPNLTSDATGIGAWTEANFVKAIKEGKHLGVGRP
                     IAPPMPWPAYRHATDEDLKAVFAYLKTVPPVKNQAPDYEPPAMARK"
BASE COUNT          600 a         1424 c         1751 g          565 t
ORIGIN      
        1 gctcgccggt gcggcgatcg ccggacggca aggccgggcg ggccggtgaa cgagcgtgcg
       61 tggaccacgc catcctgggt ccgataccgc agcggcgcag cctcgtcgtc aggtcgagga
      121 cgcccgcatc cgctccctca cctgccaggc cgccgacccg atgacgtcga gaaggcgggt
      181 gaagacggga cggaacgcct cgggcgggtc gaagaactgc tcgacgctga cccagacgac
      241 gccgtccacg aggacgcact tgacggtctt caggtcgcgg ttgacggagt tgaccgcggc
      301 gagcgcgcgg ctctcctctt cggggccgtc gcactcccag gcgccgggaa gggcggcgcg
      361 gaagtactcc gggtctttcc cgtcgcggaa gaggtagagc gtccgccctt cgtgccggaa
      421 gcggacgtcg ccgtcttcgt cgacctcggg gcgaaagccc tcggcggcga ggaaagcgaa
      481 gtacggatcg gcgtcggagg ggggcggcgg cagggaagcc tcttcggccg gcacctcggc
      541 cgaagtctcc tcgggctcgt cgacgaggtc ggggtagtcg ctcacggtct ctcctttcgt
      601 gcggcgtcgc ggtgctccgc gacgagggcg agcctggggg acggggtcgt cgcgacgccg
      661 aagccgacgg cggggcggtg ggaggcggcg cgcgcggtct cgacgcgggc ggcgtcctcg
      721 aggtcggccc gggtcaggac gcgggcgtcg cccgcgcgcg aggccgcacg ggcgagggcg
      781 tagagggccg ccgtgcggat ggccgagccc gagagcgcga accgctcggc gatcgactcc
      841 aggtcgacgt cggcggcgcg cggagcgcgc ggcgggaggt gcctttccca gaggagccgc
      901 cgctcggcgg cgccgggaag cgggaaggcg aggcgcgcgg cgaggcggcg ctcgagcgcc
      961 gggtcgagga cggcgtcgcg gttcgtcgtg aggacgacga cgccgcggaa gcgctcgatc
     1021 tcctggagga ggacgttcgt catcgtcgcc tcccaggagc gcgcggcccg ctcgcgcgtc
     1081 gtcaggaagg agtcggcctc gtcgaggagg acgacgtctc ccgtccggcg cgcctcggcg
     1141 aagacctcgg tgagccgctt ctcggtctcg ccgacgaact tggagaggag ctggtcggtc
     1201 cggaggcgcc agagggtctt cccgagctgg ccggcgatcg cctcggcgca gagcgtcttc
     1261 cccgtgccgg gcggtccgta aaggaggagg gcgacgttcg gcgccccgag gagcgtctcc
     1321 tccacgcccc agccggtctc ggagagagac gcgagcgcgg cggggacggc gagcgcctcc
     1381 gtgagacgcc gacgcgtcgc gaggggcagg acgaggccgt cgaggttcag ccgcggctcg
     1441 atccgctcga cgagagcggg gctcgcgggc acttccgacc cgatggcccg ggccgcgcgc
     1501 ggcgccggca tcccggaagg gacggcccgc agtcgggcgt cggccgtggc ccggcaggtc
     1561 gagagctgcg gcgaaccggt cgacggccgg acctccacga ggcccgcagc gacgagaggg
     1621 gaggaggggc cggggaggga agcggcgggc gacccgaggg ccggctcgag aagcccccac
     1681 gcgaggggtc cgtcgtcctc gccccggcag aggaggagaa cgaggagcgc cgcctcgggg
     1741 acggtcgtgg ctccggcctc gcggaggagg agggcgaggg gatcagccga cgcgggtccg
     1801 tcggccgaga cgtaagcgtg ggccgagcag aggagcgccc gacggacgcg ggccgcggag
     1861 tcccagccgg gcccgtcggg cacgggatag gaccagaccg tgcagcgctc gacggggtcc
     1921 ggagagaccc gggcgaggag catggccgcg gcgctgaagg cggtttgcag gcgcccgcag
     1981 ggcgtcgggg ggatctcgag cgcctctacc gcctcgctcc ccgctggaag gagccgctcc
     2041 agcggggagc gaggcggtag aggcgctcgc cgggcgcggg gtcgccgtcg gactcgacga
     2101 ggccgtggcg ccggagcgcg cccgtttcgt cgagcctcga gcagacgagc gcctggtggg
     2161 accaggtggc cgcgccggcg gcgtcgcaga gcgtcgcgat gcggaggccc ccgttcgtgc
     2221 gggcgtcgag atcgtcgcgg aggagcgcga ggaggaggag ccggtcgagg aggtcgaggc
     2281 ggaggccctc gatcgcgtcg aggacgggcg tcgggacccc cgcatcccgg gtggcggcgc
     2341 tgcgcgcggc gagggcgcga aggtggggaa ggcgcacgtc gatcgggacg atcccggtcg
     2401 ggtggtctcc tcccgtgccg gtcatcgggc cgagcgccag aaaccaggct tcgaggcact
     2461 cctcgccgga cgtgaagggt tcgatcgccg ttcgcgcgcg cttcatggtc gcctccagac
     2521 ggcacccctc ccggcgggtg cccgttcgcg gatcggtcag cgcagccggc cgccgaagtc
     2581 gtcccggccg ccggggtcga ggcggcgcag gcggcgggcc gccttcgagc cgctctcgga
     2641 gaagctcccg aggaggagga gcacgaggcc gacgaggaag agcgtggtca tggccggtct
     2701 cctttcgcct cgcagtgtcg gcccgagggt cagacaaacg gtgtccatgg atcggcgggt
     2761 cgcgcatact cctcccggga ggagccgatg gccttcaagg accgcccgta ctttcgacgg
     2821 accctcgacc tggacgacca gctgcgctcg gggaagaagg tcaccgcgac gacgctcgcc
     2881 gcgaggtggg agacgtcgac gaagacggtc cagcgtctcg tcgacggcgt gcgcgacgaa
     2941 tacggagctc cgatcgagtg ggacgcgcac agggggacgt acgtctactc ggacccgtcc
     3001 tggcacctcc cctggctcgc gatgaagggg gcggacctct tcgccgtcgg cgtggcgatg
     3061 aaggtgctgc agatgtacga ggggacgccc gtcgcgaagg acatgcgggc gatctacgag
     3121 cggctctcgg agctgatgcc cgccgaggtc cggatctcgc cctcctcgtt cgtcgagaag
     3181 ctctacatcc gcccgcaggc ccagcggccg gtcgacccga acgtctggga cgcggtcgcg
     3241 accggcctcc gggagaaggt ggcgatcgaa gccgactacc ggaagccggc cggcggcgga
     3301 ggctggcgcg agctcgagcc gctccacctc gtccttttcg ggaccgactg gttcctcctc
     3361 gcgcgggacc ccgaaggaga ggcggtgaag accttctacc tctcccggat gaaggcggtc
     3421 cgcgtcggca cgcggcgctt ctcgccgccg aggggcttcc ggccggagga gcacttcggc
     3481 gacactattg ggctctacgt cggcaagccg cgcttccgct tccgggtccg cttcggccgc
     3541 gagatcgccg agtggatcac ggaggtccgc tggcacgaga agcagaagct cgtccgccgg
     3601 agcgacggcg acgtggagct ggagctgccg gccgggtcgc tcctcgaggc gcggcgcttc
     3661 gtcctctcgt tcgggatgca cgccgaggtc ctcgggccgg aggagctcgt cgaagacgtg
     3721 aaggcgcacg tccgttcgct ggcggaacgc tacgggtagg gtgccgcgcc tcgcccgggg
     3781 cgtcggcgcc gggaagagga cgaaacggcc cggcgcgtcg tcgcgcgccg ggccgtcgtc
     3841 cgaaggctcg aggtgagatg aggtggggtg aggtgaggcg ggaggaggct tctacttccg
     3901 cgccatcgcg gggggctcgt agtcgggggc ctggttcttc acgggcggga cggtcttgag
     3961 gtaggcgaag acggccttca ggtcctcgtc ggtcgcgtgg cggtaggcgg gccagggcat
     4021 cggcggggcg atcgggcggc cgacgccgag gtgcttccct tccttgatgg ccttgacgaa
     4081 gttcgcctcg gtccaggcgc cgatcccggt ggcgtcggag gtgaggttcg gggcgtagct
     4141 gatcccccac ggcccggcga aggcggtgtt cgtggcgttg ccggcccaga tccacgcctt
     4201 cgcgtcgatc ggcgccggct tcgtcacttt caccgcctgc gggtggccgg agagccggcg
     4261 tgcgaagtcg ggctcgggcc ccttcgcgcc catcttcatc ggcgtgtgac agtcgtcaca
     4321 gcccatgatg ccgacgaggt
//
LOCUS       DAOTEZ010000379         4335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_14872_length_4335_cov_7.128738, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000379 DAOTEZ010000000
VERSION     DAOTEZ010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14872_length_4335_cov_7.128738"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="P5164_16185"
     CDS             <1..896
                     /locus_tag="P5164_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45478.1"
                     /translation="DEALEELTAVAIRLGRGARLPKDGRLEGWFRAGGEPLKVAAVEE
                     GPSEIVFVVAGAALDDLRRLRQGVLDPRTYGRRGRSLLLDDKLAQDRVFGPESAPAVV
                     LPNLPDEARYRFVETIPRVVRSPVEVASRFLSSVDYSASTDRFFSVLGRVGLEQRPGK
                     PQVAEAVAIAGLSAAEKSRRRAVVLLLGSGAEEAGTISAERARRCLSKLGVPLFVWDL
                     SRVREEAWKKPAPPTEGLADVRSHWPEAVRVDDSSSILGVFRSLAKELSSQRIVWLKG
                     RVNPEKVELTASAGKGVSLVP"
     gene            1011..1208
                     /locus_tag="P5164_16190"
     CDS             1011..1208
                     /locus_tag="P5164_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HRY45479.1"
                     /translation="MTSLTVRLTDAQAEHLALEAQKLGVTPEDLLRAAVIDLIEGRDE
                     AFLSAARHVIEKNAELYQRLA"
     gene            1205..1600
                     /locus_tag="P5164_16195"
     CDS             1205..1600
                     /locus_tag="P5164_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HRY45480.1"
                     /translation="MMRYLTIAEVLYLHRMVIAQSGSSGIRDLGGLESAIAQPLATFA
                     GSDLYSSVAEKASALCFSLVLNHPFVDGNKRVGHAAMETFLVLNGFDLSAPVSEQEEL
                     MLALAGGSLSREGLLRWVDAHIRPRVRPS"
     gene            1693..3030
                     /locus_tag="P5164_16200"
     CDS             1693..3030
                     /locus_tag="P5164_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45481.1"
                     /translation="MIAFVTLFLGLFYGPAEIELSAAPGVARIELYVDGDLVSELTGP
                     WVAKLDLGQEIVPRELVAVAKDAEGRRVGEVRQWINRPRPGAEASFVVEKEPSGAGRT
                     ARLLWRSVTRSKAKSIRVTFDGQPLEVPDPNRIPLPAHSPGTTHILVADVDFEDGLAA
                     TAVASLGGEKLDEALEELTAVPIRLDPGGSLPKDKRLEEWFRAGEKPLTVAAVEEGPS
                     DVVFVVAGRALDDLEKLLEDVTRQRSWLAVRSQANLPVGARFRFVETIPRIVRSPAEV
                     ASRFFSSEDYSAKDDYFFSAIRKVAIGNRSGDPRIADAVAIAGLSAAEKSRRRAVVLL
                     LGSGATEAGPITAERARRCLAKLGVPLFVWDVSRAPAKSEQKLDPGAEAQADARAHWP
                     EAVRVSGLTSLLGVFRSLEKELASQRIVWLKGRLNPAKVELTASAGKGVSLVQ"
     gene            3027..4070
                     /locus_tag="P5164_16205"
     CDS             3027..4070
                     /locus_tag="P5164_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRY45482.1"
                     /translation="MRAASRPLSVLLAALGLLAGRPAAAEHVTLPLGARLHLEPDVKS
                     HSLALLDAEALVEVLERKDGWILVRYDAFRGWVREGAPRSLVEERGNSYSAQGVVHEG
                     LLARGRVLLRGGGRELPMDGRTLLTDVSDEALLKWLVAMPAAARYIFEKKWGRTRAPA
                     PGSVVFLFESAEAEREFSSVACGATELLGERIASLALSGKGRAWDRARLLHQIGHVYA
                     RDVLGEGVPAWIEEGFADEFARAGIPVEIVEGWRAVHYPKRSLPSPPVATVPEILAAG
                     AEIFGREPAAEARRLEALLFFRYLAGDRKFLPFRDFVTSGGKLDPPALEEALRRKIGA
                     IERGYLSARPGIR"
     gene            4107..>4335
                     /locus_tag="P5164_16210"
     CDS             4107..>4335
                     /locus_tag="P5164_16210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45483.1"
                     /translation="MIAFVTLFLGLVYGPVTVELSAAPGVARIELSVDGEIVAELEAP
                     WTARLDLGPEIAPRELVAVAKDAEGRRVGEVR"
BASE COUNT          587 a         1513 c         1555 g          680 t
ORIGIN      
        1 tcgacgaggc gctcgaggag ctgacggcgg tggcgatccg cctcggccgc ggcgcccggc
       61 tcccgaagga cgggcggctc gaagggtggt tccgggcggg cggagaacca ctgaaggtcg
      121 ccgcggtcga ggaagggccg tcggagatcg tcttcgtcgt ggcgggggcg gccctcgacg
      181 acctgcgccg gcttcggcaa ggcgttctgg acccccgcac atacggtcgg cgcggccgtt
      241 cgttgctcct cgacgacaag ctcgcgcagg accgcgtttt cgggccggag agcgcccctg
      301 ccgtcgtcct ccccaacctt ccggacgagg cgcgctatcg cttcgtggag acgatcccgc
      361 gtgtcgtccg cagccccgtc gaggtcgcga gccgcttcct ctcctccgtc gactactccg
      421 cgagcaccga ccgattcttc tccgtcctcg gcagggtggg cctcgagcag cggcccggaa
      481 agccgcaggt cgccgaggcc gtggcgatcg ccggcctttc ggccgcggag aagagccggc
      541 gccgggccgt cgtcctcctc ctcgggagtg gcgccgagga agcggggacg atctcggccg
      601 agcgcgcccg gcgctgcctc tcgaagctcg gggtcccgct cttcgtctgg gacctctcgc
      661 gggttcggga agaggcctgg aagaaacccg cccctccgac ggaggggctc gcggacgtgc
      721 gctcccactg gccggaggcg gtgcgcgtgg acgactcgtc gtcgatcctg ggcgtgttcc
      781 ggtccctcgc gaaggagctc tcgtcccagc ggatcgtctg gctgaagggg cgcgtcaacc
      841 cggagaaggt cgagctgacc gcgtcggcgg ggaagggggt ctcgctcgtg ccatgagagc
      901 cgtctcgcga ccggcgggtt cgcgaagcgg ctcgtcaggt gacggggcac ggcccgtcct
      961 cgccgcctca gagggtcagt ggccaggggc ctgagcgcta gaatcgtgtc gtgacgagcc
     1021 tgaccgtgcg cctcaccgat gcccaggccg aacatctcgc gctcgaggcg cagaagctgg
     1081 gtgtcactcc tgaggatctc ctccgagccg cggtcataga cttgatagag ggtcgagacg
     1141 aggcgttcct gtccgccgcg cgccacgtca tcgagaagaa cgccgagctg taccaacggc
     1201 tcgcctgatg cggtatctca ccatcgcgga ggtgctctac ctccatcgca tggtgatcgc
     1261 tcagtccggc tcctcgggca ttcgtgatct gggcggcctc gagtcggcga tagcccagcc
     1321 cttggcgacg ttcgcaggct cggacctcta ctcgagcgtg gcggagaagg cctcggcgct
     1381 ctgcttctcg ctcgtgctca atcacccgtt cgtggacggc aacaagcgcg tggggcacgc
     1441 ggcgatggag acgtttctcg ttctgaacgg ttttgacctc tcggccccgg tgtccgagca
     1501 ggaggagctg atgctcgccc tcgcaggcgg ttccctgtcc cgcgaagggc tgctccgctg
     1561 ggtcgacgcg cacatccgtc cccgagttcg accctcctga ttcgccgcgc ctgccccgct
     1621 ccgcctctgt ccccgcccgg agacggcccc cggggaacgc gaattgcttg aactctgccg
     1681 ggaggagtcc gcgtgatcgc tttcgtgacg ctgttcctcg gcctcttcta cgggccggcc
     1741 gagatcgagc tctccgccgc gccgggcgtg gcgcggatcg agctctacgt cgacggcgac
     1801 ctcgtctcgg agctgacggg gccgtgggtc gcgaagctcg acctcgggca ggagatcgtt
     1861 ccgcgggagc tcgtcgccgt ggcgaaggac gccgaggggc gccgggtcgg agaggtccgc
     1921 cagtggatca accgcccgcg gcccggcgcg gaggcgagct tcgtcgtcga gaaggagccg
     1981 agcggcgcgg gccggacggc gcggctcctc tggcggagcg tcacgcggtc gaaggcgaag
     2041 tcgatccgcg tcaccttcga cggccagccg ctcgaggttc ccgacccgaa ccggatcccg
     2101 cttccggccc actctccggg gacgacgcac atcctcgtcg ccgacgtcga cttcgaggac
     2161 ggcctcgcgg ccaccgccgt cgcgagcctc ggaggcgaga agctcgacga ggcgctcgag
     2221 gagctgacgg cggtcccgat ccgcctcgac ccgggcggca gcctcccgaa ggacaagcgg
     2281 ctcgaagagt ggttccgggc gggagagaag ccgctgacgg tcgccgcggt ggaagagggg
     2341 ccttccgacg tcgtcttcgt cgtggcgggc cgcgcactcg acgacctgga gaaactcctt
     2401 gaagacgtca cccgccagag gtcgtggttg gcggtgcgct cgcaggccaa cctgcctgtc
     2461 ggagcgcgat ttcgcttcgt cgagacgatc ccgcgcatcg tccgcagccc cgcggaggtc
     2521 gcgagccgct tcttctcctc cgaggactat tccgcgaagg acgactactt cttctccgcc
     2581 atccgcaagg tcgccatcgg gaatcgatcc ggggatcccc gcattgccga cgccgttgcg
     2641 atcgccggcc tctcggcggc ggagaagagc cggcggcggg ccgtcgtcct cctcctcggg
     2701 agcggcgcca ccgaagcggg gccgattacg gccgagcgcg cccggcgctg cctcgcgaag
     2761 ctcggagtcc ccctcttcgt ctgggacgtc tcgcgggcgc cagcgaagag cgagcagaaa
     2821 ctcgaccctg gagcggaggc gcaggccgac gcgcgcgccc actggccgga ggcggtgcgc
     2881 gtctcgggcc tgacgtcgct cctgggcgtc ttccggtcgc tcgagaagga gctcgcgtcc
     2941 cagcggatcg tctggctgaa gggacgcctc aacccggcga aggtcgagct gacggcgtcg
     3001 gccggaaagg gagtctcgct cgtccaatga gagccgcatc gcgccctctc tccgtcctcc
     3061 tcgccgcgct cgggctcctc gccgggcgtc ccgcggccgc ggaacacgtc accctgccgc
     3121 tgggcgcccg gctccacctc gagcccgacg tgaagtcgca ttccctcgcc ctcctcgacg
     3181 cggaggcgct cgtcgaggtg ctcgagagga aggacggctg gatcctcgtc cggtacgacg
     3241 cgttccgcgg ctgggtgagg gagggtgcgc cgagatcgct cgtcgaagaa cgcggcaatt
     3301 cgtactcagc gcagggggtg gttcacgagg gactcctcgc gcgcggccgg gtcctcctgc
     3361 gcggcggcgg gcgcgagctc ccgatggacg gccggaccct tctcaccgac gtctccgacg
     3421 aggcgctcct gaagtggctc gtcgcgatgc cggcggcggc gcggtacatc ttcgagaaga
     3481 aatgggggag gacccgagcc ccggcgccgg ggtcggtcgt cttcctcttc gaatcggccg
     3541 aggccgagag ggagttctct tccgtcgcct gcggagcgac ggagctgctt ggcgagagga
     3601 tcgcgtcgct cgccctttcc ggaaaggggc gggcctggga ccgggcgcgc ctccttcacc
     3661 aaatcgggca cgtctacgcg agagacgtac tgggggaggg cgtccccgcc tggatcgagg
     3721 aaggtttcgc cgacgagttc gcccgggcgg gaatccccgt cgagatcgtc gagggctgga
     3781 gggccgtcca ctacccgaag cgctcgctcc cgtcgccccc ggtcgcgacg gtccccgaga
     3841 ttctcgccgc cggcgcggag atcttcggga gggagcccgc ggccgaggcg cggcgcctcg
     3901 aggcgctcct cttcttccgc taccttgcgg gagaccgcaa gttcctcccg ttcagggact
     3961 tcgttacgtc gggagggaag ctcgatcccc cggcgctcga ggaggctctt cgacggaaga
     4021 tcggagcgat cgagcggggc tacctctccg cccggccggg gatccgatag tcgcccgagg
     4081 tccgcgcgaa gcacggagga gtccacgtga tcgctttcgt gaccttgttc ctcggcctcg
     4141 tctacggccc cgtcaccgtg gagctctccg ccgcgcccgg cgtcgcacgg atcgagctct
     4201 cggtcgacgg cgagatcgtc gcggagctcg aggctccctg gaccgcccgg ctcgacctcg
     4261 ggcctgagat tgccccgcgc gagctcgtcg ccgtggcgaa ggacgccgag gggcgccggg
     4321 tcggagaggt ccggc
//
LOCUS       DAOTEZ010000380         4332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_14889_length_4332_cov_6.255553, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000380 DAOTEZ010000000
VERSION     DAOTEZ010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14889_length_4332_cov_6.255553"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..782)
                     /gene="nadD"
                     /locus_tag="P5164_16215"
     CDS             complement(120..782)
                     /gene="nadD"
                     /locus_tag="P5164_16215"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HRY45484.1"
                     /translation="MLSRTHLLGVYGGSFDPIHLGHLVPVEEARAATGLDEILYVPAF
                     APPHKPQGPSASAFHRFGMCATALEAYPHLLLSDFEVSRGGTTYTIETLRHVRANHPE
                     SEVFLVLGSDSMAQLHTWREWKEIVSSHRIVVVYREGVDYPDLREGLPPELAARLAPL
                     GARPHDELSEQQTIFWAGNAPVTISSTWIRSALPSVQSLSESLPPAVERYARRHRLYV
                     AP"
     gene            complement(775..1836)
                     /gene="obgE"
                     /locus_tag="P5164_16220"
     CDS             complement(775..1836)
                     /gene="obgE"
                     /locus_tag="P5164_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRY45485.1"
                     /translation="MFLDEASIQVSAGDGGNGCVAFRRAKGEPHGGPNGGDGGDGGSV
                     RIVADEGLNTLYPFRFKKRFAAGRGQHGMGSNCTGKKGGDIEITVPVGTVVKDADSGE
                     VLGDLAENGQVLLVARGGRGGRGNQHFATPTHRTPRRAEPGTEGERRSLALELKLLAD
                     VALVGFPNAGKSTLIAAISAARPKIADYPFTTLVPNLGVVSWERYKTFVAADVPGLIE
                     GASDGHGLGIRFLRHVERSRLLCHLVDAAALDPEDPEGAAEKAVSVIEGELEAFAPEL
                     AARERVLCANKIEIASEAQVAAVRAAASRRGLRFFAISGAAHKGLDPLVSFLGGRLEA
                     MSVRPAPVLPVPAGEAPDA"
     gene            complement(1891..2178)
                     /gene="rpmA"
                     /locus_tag="P5164_16225"
     CDS             complement(1891..2178)
                     /gene="rpmA"
                     /locus_tag="P5164_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRY45486.1"
                     /translation="MAHKKGQGSSRNGRDSNAQRLGVKRFAGQWVTGGSILVRQRGTP
                     LKPGENVGRAKDDSLFAMVDGVVDFKDKGRLGRYVSVTPGPRPGSEAAAAS"
     gene            complement(2200..2520)
                     /gene="rplU"
                     /locus_tag="P5164_16230"
     CDS             complement(2200..2520)
                     /gene="rplU"
                     /locus_tag="P5164_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006598024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRY45487.1"
                     /translation="MYAVIRTGGKQVRVAKGDVIQIERFHNKTFTKGDALELPEVLAV
                     GEGETLRLGSPLVTGATVHGVVVREMRGQKLIVYKKKKRKGYQRSHGHRQNLVEVRID
                     SISV"
     gene            complement(2677..3615)
                     /gene="trxB"
                     /locus_tag="P5164_16235"
     CDS             complement(2677..3615)
                     /gene="trxB"
                     /locus_tag="P5164_16235"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRY45488.1"
                     /translation="MPNKSHYAVAIIGSGPAGLTAAIYASRASLEPVVFEGVQPGGQL
                     TTTTEVENYPGFEHGIQGPELMDVTRRQAVRFGTEIANDTVVDADLSARPFTLALESG
                     AKVTCDALIVASGASAKYLGLPSEKKLMGHGVSACATCDGFFFRGKEVAIVGGGDTAM
                     EEANFLTKFATKVWLVHRRNEFRASKIMAQRTLDNPKIEVVWDSTITEVLGEPETGVS
                     GIRVKNVKTSEERVIPVQGYFSAIGHEPNTKIFRGKLDMDDVGYLKIQHPTTYTSVEG
                     VFAAGDVADHIYRQAISAAGEGCKAAIDAERWLAGK"
     gene            3784..4272
                     /gene="sixA"
                     /locus_tag="P5164_16240"
     CDS             3784..4272
                     /gene="sixA"
                     /locus_tag="P5164_16240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HRY45489.1"
                     /translation="MLLYLLRHAEAEALSSSGLDADRALTGAGQKRMKAVGRAIARME
                     PGFDAVLVSPLLRAKETAGFVTSACRFEGEAVVTESLLPGSDPELLLEELSASEWRTV
                     LLVGHEPHLGRLFGRLVSGRRETEVPMRKASLAVFEVHGHLGPDRAELKSYLPARLLE
                     RL"
BASE COUNT          592 a         1452 c         1600 g          688 t
ORIGIN      
        1 cggagggggc gggagcgcgg gcttgcgccg cggagccggg ggccgcgcgc cggtggcctt
       61 cgcctggacc gggcccttgg ccttcgcggc gggacgcttc gcggcgggct tcgcgccctt
      121 cacggcgcga cgtagagccg gtgccgcctc gcgtaccgct ccaccgcggg aggaagactt
      181 tcgctcaggc tttggacgct cgggagtgcg cttcttatcc acgtggagga gattgtaacc
      241 ggggcgttcc ccgcccagaa gatggtctgc tgctcggaga gctcgtcgtg aggccgcgcg
      301 ccgagcgggg cgagccgggc cgcgagctcg ggcgggagcc cctcgcgcag gtccgggtag
      361 tcgaccccct cgcggtagac gaccacgatg cggtgggagg agacgatctc cttccactcg
      421 cgccaggtgt gcagctgggc catcgagtcg gagccgagga cgaggaagac ctcgctctcg
      481 ggatggttgg cgcggacgtg ccggagcgtc tcgatggtgt aggtcgtccc gccgcgcgag
      541 acctcgaagt ccgagaggag gaggtgcggg tacgcctcga gcgcggtcgc gcacatcccg
      601 aagcggtgga acgccgaagc ggaggggccc tgcggcttgt ggggcggggc gaaggcgggg
      661 acgtagagga tctcgtccag gccggtcgcc gcacgcgcct cctcgacggg aacgaggtgg
      721 ccgaggtgga tcgggtcgaa cgagccgccg tagacgccga ggaggtgggt tcggctaagc
      781 atccggggcc tcccctgccg gcacgggcag gacgggggcc ggcctcacgc tcatcgcctc
      841 gaggcggccg cccaggaagg agacgagcgg gtcgaggccc ttgtgggccg cgcccgagat
      901 cgcgaagaag cggaggccgc ggcgcgaggc cgccgcgcgg acggcggcga cctgcgcctc
      961 ggaggcgatc tcgatcttgt tcgcgcagag gacgcgctcg cgcgccgcga gctccggcgc
     1021 gaacgcctcg agctctccct cgatgaccga gacggccttc tcggcggccc cctcggggtc
     1081 ctccgggtcg agggccgcgg cgtcgacgag gtggcagagg aggcgcgagc gctcgacgtg
     1141 gcgaaggaag cggatgccga ggccgtgccc gtcgctcgct ccctcgatca ggccggggac
     1201 gtcggccgcg acgaacgtct tgtagcgctc ccaggagacg acgccgaggt tcgggacgag
     1261 cgtggtgaag gggtagtcgg cgatcttcgg gcgcgccgcg gagatggcgg cgatgagcgt
     1321 cgacttgccg gcgttcggga agccgacgag ggcgacgtcg gcgaggagct tcagctcgag
     1381 cgcgagcgag cgccgctcgc cctcggtgcc gggctcggcg cgccgcggcg tgcggtgggt
     1441 gggcgtggcg aagtgctggt ttccgcgtcc gccgcggccg ccccgggcca cgaggaggac
     1501 ctggccgttc tcggcgagat cgccgaggac ctcgccggag tcggcgtcct tgacgaccgt
     1561 cccgacgggg acggtgatct cgatgtcgcc gccctttttc ccggtgcagt tcgagcccat
     1621 gccgtgctgg ccgcgcccgg cggcgaaccg cttcttgaag cggaacgggt agagggtgtt
     1681 gaggccctcg tccgcgacga tgcggacgct gcccccgtcg ccgccgtcac cgccgttggg
     1741 accgccgtga ggctcgccct tggcacgccg gaacgcgacg cagccgttgc cgccgtcacc
     1801 ggccgagacc tgtatcgacg cctcgtcgag gaacatatcg tccgtgccgg agggtcgggt
     1861 ccggggcgcc cgagggcgcc ccgcccggaa tcagctcgcg gccgcggcct cggagccggg
     1921 acgcggaccc ggcgtcacgc tgacgtaacg accgaggcgg cccttgtcct tgaagtcgac
     1981 gacgccgtcg accatcgcga agagcgagtc gtccttggcg cggccgacgt tctcacccgg
     2041 cttgagcggg gtgccccgct gccggacgag gatcgagccg ccggtgaccc actgacccgc
     2101 gaagcgcttc acgccgaggc gctgggcgtt cgagtcgcgg ccgttgcggg aagaaccctg
     2161 accttttttg tgagccatag cagcttcctc ggaaggctct cagaccgaga tcgagtcgat
     2221 ccggacttcg acgaggttct ggcggtgccc gtggctccgc tggtacccct tgcgcttctt
     2281 cttcttgtag acgatgagct tctggcccct catctcgcgg acgacgacac cgtggacggt
     2341 ggctccggtc acgagaggag agccgaggcg gagggtctcc ccctcgccga cggccaggac
     2401 ctcgggcagc tcgagcgcat cgcccttggt gaaggtcttg ttgtggaagc gctcgatctg
     2461 gatgacgtcg cccttggcga ccctgacctg cttccctccg gtgcgaatga cggcgtacat
     2521 cgattcctcc cccgacctcg ggtcaaaagg gtcggcaaac atacccgccc ccgccccgaa
     2581 tgtcaaagcc cgcgagagcg aggggggtct ggtctacact ctacactctg cttgccggca
     2641 aagcgtagag tgtcgacgag ccccccccgc gtcgccctac ttcccggcca gccagcgctc
     2701 ggcgtcgatg gcggccttgc agccctctcc cgctgcgctg atggcctgac ggtagatatg
     2761 gtcggcgacg tctcctgccg cgaagacccc ctcgaccgag gtgtacgtcg tcgggtgctg
     2821 gatcttcagg tagccgacgt cgtccatgtc gagcttgccc cggaagatct tcgtgttcgg
     2881 ctcgtggccg atggccgaga agtagccctg gacggggatg acgcgctcct cgctcgtctt
     2941 cacgttcttc acgcggatgc cgctcacgcc cgtctcgggc tcgccgagga cctcggtgat
     3001 ggtcgagtcc cagacgacct cgatcttcgg gttgtcgagc gtgcgctggg ccatgatctt
     3061 cgaggcgcgg aactcgttcc ggcggtggac gagccagacc ttcgtcgcga acttcgtcag
     3121 gaagttcgcc tcctccatcg cggtgtcgcc gccgccgacg atcgcgacct ccttgccgcg
     3181 gaagaagaag ccgtcgcacg tggcgcaggc cgagacgccg tgccccatga gcttcttctc
     3241 gctcgggagg ccgaggtact tggccgaggc cccggaggcg acgatgaggg cgtcgcaggt
     3301 caccttcgcg ccgctctcga gcgcgagcgt gaaggggcgg gcggagaggt cggcgtcgac
     3361 gacggtgtcg ttcgcgatct cggtgccgaa gcggaccgcc tgcctgcggg tgacgtccat
     3421 cagctccggg ccctggattc cgtgctcgaa gccggggtag ttctccacct ccgtcgtcgt
     3481 cgtgagctgg ccgccgggct ggaccccctc gaagacgacc ggctcgaggc tcgcgcggga
     3541 agcgtagatc gcggcggtca ggcccgcggg gcccgatccg atgatggcga cggcgtaatg
     3601 gctcttgttc ggcatctctt cctcggttcg gtgcggcgcc ggggcgtcgc aaagtgtaga
     3661 tagtagacct gaccccaagg aggataccaa ggatcgaagg gccgcaggga tcgtccccgg
     3721 ggcgagcgat tctgcacccg gggggcggcc cggggcgcgc ggggcgtgtg atagcgtttt
     3781 cccatgcttc tctacctgct gcgtcacgcc gaggcggagg ccctctcctc gagcggcctg
     3841 gacgccgacc gggcgctcac cggggcgggt cagaagcgga tgaaggcggt gggccgggcg
     3901 atcgcacgca tggagcccgg attcgacgcg gtcctcgtgt cgcccctcct gcgggcgaag
     3961 gaaacggccg ggttcgtgac gtccgcctgc cggttcgagg gcgaggccgt cgtgaccgag
     4021 tcgctcctgc cgggatccga cccggagctc ctgctcgagg agctctccgc gtcggagtgg
     4081 cggacggtcc tcctcgtcgg acacgagcct cacctcggac gtctcttcgg ccgtctcgtc
     4141 tcgggccgga gggagacgga ggtgccgatg aggaaggcgt ctctcgccgt cttcgaggtc
     4201 cacggccacc tcggccccga tcgtgccgag ctgaagtcct acctccccgc gcgcctcctc
     4261 gagcgcctct gaccggcgct tctcgaggca gaggccaggg gtctggtcta cactctacag
     4321 tctgctgagc cg
//
LOCUS       DAOTEZ010000381         4303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_15014_length_4303_cov_7.153249, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000381 DAOTEZ010000000
VERSION     DAOTEZ010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15014_length_4303_cov_7.153249"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="P5164_16245"
     CDS             <1..552
                     /locus_tag="P5164_16245"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HRY45490.1"
                     /translation="LVLHPGSHGGDGEGVGLDRIARGLDEAAAKCPSSGTTVLLETAA
                     GQGACLGHDFAHLAAIFERADSRPRLALCFDTCHVHAAGYDVVTKDGWRKTLDELDAS
                     CGLGLVKVIHANDSKKERGCRVDRHERIGHGAIGLKGFANLMTERAFEEVPKILETPK
                     DEEGTWDRDGLAALRKLARRKAG"
     gene            complement(757..1737)
                     /locus_tag="P5164_16250"
     CDS             complement(757..1737)
                     /locus_tag="P5164_16250"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HRY45491.1"
                     /translation="MMDLQTTWFLVVGVLFAGYSVLDAIDGGVGTLVLSRPGDDETRR
                     RRLGTLGPATLGNELWLVAGLAALFAAFPPVRAAVTSAFGGLLALLALVFVLRAVALG
                     RGRGPEPADRSRDVAFGALSAPAAFLLGLMLGNVLLGLPLDEAGRSTASFGDLLSPFA
                     LVVAADAVALFALQGACWLNLRAGEGEDVVLRAWVTAVALHGLTGVSSLFAAPHLWEP
                     YHHPLTWIAPVVMLASLVALPYFVKAGRPALALGASSLSIAALWGIVGQGLYPALVPG
                     RGGAGESLTIASSAASPDVLTSLLVVLLLALTLVAGYSAFVFLRFRRSAA"
     gene            complement(1846..3498)
                     /locus_tag="P5164_16255"
     CDS             complement(1846..3498)
                     /locus_tag="P5164_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013374605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRY45492.1"
                     /translation="MRSTRLALVVPMALAALTCGSSPSEREVVPAPSRAASAGWDHEW
                     ARGAVFYEVFVRSFQDSDGDGIGDLPGLISRLDDLNDGDPATTTDLGVDALWLMPVFE
                     SPSYHGYDTTDYLKIEPDYGTNADFLRLLDEAHRRGMRVIVDLVVNHSSSQHPWFVAA
                     SSSPASPYRDWYVWSATDPGWKQPWGGNAGTWHRSGDAFYYGVFWSGMPDLNWRSASL
                     RGEMRRISALWLERGVDGFRLDATRYLVEAGAGGGQADTPETHAALKAFAAHVRRVKP
                     QAFVVGENWADTSVIATYYGSKSVPGGDELPASFNFPLSDLVLKAVSSGNAAPVAAKL
                     EEVGRVYPAGAIDAPFLTNHDQVRLATQLGRNAGRLRSAAAILLTLPGAPFLYYGEEV
                     GLENGTTGGDESKRTPMPWDGTPGGGFTTGTPWFPFAPGRETANVAALKNDPSSLHSR
                     YRNLIRARASSEALRKGETTLVSRTDGVTPFLAFLRRSAGETVLVVHNLSDSFQSAGP
                     FVVPGSTFERVFADPGAADPSGAPEAIRVYLPGRGTGIWRVR"
     gene            3549..>4303
                     /locus_tag="P5164_16260"
     CDS             3549..>4303
                     /locus_tag="P5164_16260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45493.1"
                     /translation="MSGFDAARLGALVHDLLAVVRRRPADLLPFEEVRERLKLSRVVD
                     RGTSDVPLEKIVGTFGRRGEFNRAFLPRSEALRERWEDVKDLAEGAAGFPPVELYLVG
                     DAYFAVDGHHRVSVARSLGARTIEAHVLEFLTPVPLGPDASLEDVLRKEGLADFLEAT
                     GLVPSSPDEFRVTHPGDYAEMLEHIAVHRYFLGQEHSRCASWEEAVRSWYETFFRPMT
                     ETIRASGVLADFPARTEADLYLFVARHLHALRER"
BASE COUNT          624 a         1558 c         1590 g          531 t
ORIGIN      
        1 ctcgtcctcc accccggctc gcacggcggc gacggcgagg gggtcggcct cgaccggatc
       61 gcccgcggcc tcgacgaggc cgcggcgaag tgcccgtcct cggggaccac cgtcctcctc
      121 gagacggccg ccggccaggg ggcctgtctc ggccacgact tcgcgcacct cgcggcgatc
      181 ttcgagcgcg ccgacagccg cccgcgcctc gccctctgct tcgacacctg ccacgtccac
      241 gccgccggct acgacgtcgt cacgaaggac ggctggcgga agacgctcga cgagctcgac
      301 gcctcctgcg ggctcggcct cgtgaaggtg atccacgcga acgactcgaa gaaggagcgc
      361 ggctgccgcg tcgaccgcca cgagcggatc ggccacggag cgatcggcct gaagggtttc
      421 gcgaacctga tgaccgagcg cgccttcgag gaggtcccga agatcctcga gaccccgaag
      481 gacgaggagg ggacgtggga ccgcgacggg ctcgccgccc tgaggaagct cgcgcgccga
      541 aaggccggct gaaaggggcc ggggaccgga gaccgcgggc cgccccgcgc cgacctttcg
      601 tcgattcctc gggggccgct cccggggtcg cctgtgagac gccccgcccc tcctgctgcc
      661 ccccggggat gggggtctgg tctacacttt acagtctgct ggccagggga caccgagcgc
      721 gaacgccctc cgcggggatc ggcgtgcgcg ggaccctcag gccgccgagc gccggaagcg
      781 gaggaagacg aacgcgctgt agcccgcgac gagcgtgagg gcgaggagga ggacgacgag
      841 gagcgaggtc aggacgtccg ggctcgccgc ggagctcgcg atcgtcaggc tctcgccggc
      901 gccgccccgc cccgggacga gcgcgggata gagcccctgc ccgacgatcc cccagagggc
      961 ggcgatcgag agcgaggagg ccccgagcgc gagggccggc cgccccgcct tcacgaagta
     1021 cgggagcgcg acgagggacg cgagcatcac gacgggcgcg atccaggtga gcgggtggtg
     1081 atacggctcc cagaggtggg gcgcggcgaa gagcgaggag acgccggtga ggccgtggag
     1141 cgcgaccgcg gtcacccagg cgcgcaggac gacgtcttcg ccctcgcccg cgcggaggtt
     1201 cagccagcag gccccctgga gcgcgaagag ggcgacggca tccgccgcca cgacgagcgc
     1261 gaacggcgac agcaggtctc cgaacgaggc cgtgcttcgc cccgcctcgt cgagcgggag
     1321 gccgaggaga acgttcccga gcatcaggcc gaggaggaag gcggcggggg cgctcagcgc
     1381 cccgaacgcg acgtcgcgag acctgtcggc gggctccggc ccccttcccc tgccgagcgc
     1441 cacggcccgg aggacgaaga cgagcgcgag gagggcgagc aggccgccga aggccgacgt
     1501 caccgccgcg cggaccggcg ggaacgcggc gaagagggcc gcgagccctg cgacgagcca
     1561 gagctcgttc ccgagcgtgg ccgggccgag agtgccgagc cgcctccggc gcgtttcgtc
     1621 gtcccccggc cgcgacagca cgagcgtccc gacgccgccg tcgatcgcgt cgagcaccga
     1681 gtacccggcg aagaggacgc cgaccacgag gaaccacgtc gtctgcaggt ccatcatcgg
     1741 agcacctcca cccggctgcg gtttcgccct caggttagcg ccggcgtcgg cgcggggaca
     1801 gagagaagcg cggttcccgg gccgagctct ccccggggcg tcccctcagc ggacgcgcca
     1861 gatccccgtg ccgcgccccg gcaggtagac gcgaatcgcc tcgggggcgc cgctcgggtc
     1921 cgccgcgccc ggatcggcga agacccgctc gaacgtgctc cccggcacga cgaacgggcc
     1981 ggccgactgg aaggaatccg acaggttgtg gacgacgagg acggtctccc cggcggacct
     2041 ccggaggaac gcgaggaagg gcgtgacccc gtccgtcctc gagacgaggg tggtctcgcc
     2101 cttccggaga gcctccgacg aggcccgcgc gcggatgagg ttccggtatc gcgagtggag
     2161 cgaggagggg tcgttcttca gcgcggcgac gttcgcggtc tcgcgccccg gagcgaacgg
     2221 gaaccagggc gtcccggtcg tgaaaccgcc gccgggtgtc ccgtcccacg gcatcggcgt
     2281 ccgcttcgac tcgtcgcccc cggtcgtccc gttctcgagg ccgacctcct cgccgtagta
     2341 gaggaacggc gcgcccggca gcgtcaggag gatcgccgca gcgctccgga gacgccccgc
     2401 gttccggccg agctgcgtcg cgaggcgcac ctggtcgtgg ttcgtcagga acggggcgtc
     2461 gatcgcgccg gccggataga cccggcccac ctcctcgagc ttcgccgcga ccggcgcggc
     2521 gttgccggag gagaccgcct tgaggaccag gtcggagagc gggaagttga agctcgcggg
     2581 gagctcgtcc ccgcccggga cggacttcga gccgtagtag gtcgcgatca cggaggtgtc
     2641 ggcccagttc tctccgacga cgaacgcctg cggcttcacg cgccggacgt gcgccgcgaa
     2701 cgccttcagc gccgcgtgcg tctcgggcgt gtcggcctgc cctccccccg cgcccgcctc
     2761 gacgaggtag cgcgtcgcgt cgaggcggaa tccgtcgacg ccccgctcga gccagagggc
     2821 cgagatccgc cgcatctccc cgcggaggct cgcgctccgc cagttcaggt cgggcatgcc
     2881 gctccagaag actccgtagt agaaggcgtc gccgctccgg tgccaggtcc cggcgttgcc
     2941 gccccacggc tgcttccagc ccgggtccgt tgcgctccag acgtaccagt cgcggtaggg
     3001 cgacgccggc gaggaggagg ccgccacgaa ccacgggtgc tgcgagctgc tgtggttgac
     3061 gacgaggtcg acgatgaccc gcatcccccg ccggtgcgcc tcgtcgagga gccgcaggaa
     3121 gtcggcgttc gtgccatagt ccggctcgat cttcaggtag tcggtggtgt cgtagccgtg
     3181 gtagctcggg gactcgaaga ccggcatgag ccagagggcg tcgacgccga ggtcggtcgt
     3241 cgtcgccggg tcgccgtcgt tgaggtcgtc gaggcgcgag atcaagcccg gcaggtcgcc
     3301 gatgccgtcg ccatcggagt cctggaagct ccggacgaag acctcgtaga agacggcgcc
     3361 gcgggcccac tcgtggtccc agcccgccga ggccgcgcgg gagggagccg ggacgacctc
     3421 cctctcggac ggcgagctgc cgcaggtgag ggcggcgagc gccatcggga caacgagcgc
     3481 gagtcgcgtc gaacgcatgg ggacctccgg gccgtggccc gcgggaagca tatcggaggg
     3541 gcgagaccgt gagcggcttc gacgcggccc gcctcggcgc cctcgtgcac gacctcctcg
     3601 ccgtcgtgag gcggcgcccg gccgacctcc tcccgttcga ggaggtccgc gagcgcctca
     3661 agctcagccg cgtcgtcgac cgcgggacgt ccgacgtgcc gctcgagaag atcgtcggaa
     3721 cgttcgggcg gcgcggcgag ttcaaccggg cgttccttcc ccgttccgag gcgctccggg
     3781 agcggtggga ggacgtgaag gacctcgccg agggggccgc ggggttccct cccgtcgagc
     3841 tctacctcgt cggggacgcc tacttcgccg tcgacgggca ccaccgcgtc tccgtggccc
     3901 gctcgctcgg cgcgcggacg atcgaggcgc acgtcctcga gttcctcacg cccgtccctc
     3961 tcggccccga cgcctcgctc gaggacgtcc tccggaagga ggggctggcc gacttcctcg
     4021 aggcgaccgg cctcgtcccg tcgtctcccg acgagttccg ggtgacccac ccgggcgact
     4081 acgcggagat gctcgagcac atcgcggtcc accgctactt cctcggccag gagcactcgc
     4141 ggtgcgcgtc gtgggaggag gccgtgcgct cctggtacga gacgttcttc cgcccgatga
     4201 cggagacgat ccgcgcgagc ggcgtcctcg cggatttccc cgctcgaacg gaggcggacc
     4261 tctacctctt cgtcgcccgg cacctccacg cgctccggga gcg
//
LOCUS       DAOTEZ010000382         4299 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_15038_length_4299_cov_5.691800, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000382 DAOTEZ010000000
VERSION     DAOTEZ010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15038_length_4299_cov_5.691800"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="P5164_16265"
     CDS             complement(<1..496)
                     /locus_tag="P5164_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HRY45494.1"
                     /translation="MAYTAKSYDHLLGTPGFSDALMKNHFTLYQGYVTNTNKVNDELA
                     ALAADGKAATPAFAEMKRRLGWEWNGMRLHEYYFGNMKNGGAALDGGSALAKKLAADF
                     GSVEAWEKNFRAVGAMRGIGWTALYQDPVSGQLMNMWIGEHDGGHPAGGTLLMIMDVF
                     EHAFM"
     gene            complement(582..2186)
                     /locus_tag="P5164_16270"
     CDS             complement(582..2186)
                     /locus_tag="P5164_16270"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HRY45495.1"
                     /translation="MPPVSFVLVLHGHMPAVLGHGVWPHGADWLHEAAAETYLPFVAA
                     ARALEAEGVPWKATVGLTPILCEQLRDPRFPRSFRRYVTHRLRAAGRDAREFARDGHV
                     AEPLARAWRAHYEEGLALFDAFGGDVVAPFARLAEAGRIEAMASAATHGFLPLLPSDA
                     AAERQVDVGLAAHRRHFGRAAAGFWLPECATAPAGTLATPGYGRRVARRSTSSLLASR
                     GLAYTFVDTHLVAGPSAGTAAGGSAGRRWPAAGGGPAARSPYRVHRVRTDAGSLALLA
                     RDPRSSEQVWSGEVGYPGDPAYLEFHRKAARGGLRYWSVTDARGPLDAKALYEPEETR
                     ERVVAHATHFCDLLEETARAERGARVIVAMFDFELFGHWWFEGIAFLGAVFRELARRD
                     GPVRAETAGEAVREAGADAPEVEPGAGSWGRGGDFRVWDDAETKPFWQSVEEAEAQLR
                     RIEARDPGLLPAALRQALLLQASDWPFLVDGGTARDYAERRIEEHAGALRALAELAGR
                     RRSRADALFLARLARLDRAFRPELGG"
     gene            complement(2250..3530)
                     /locus_tag="P5164_16275"
     CDS             complement(2250..3530)
                     /locus_tag="P5164_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015176220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRY45496.1"
                     /translation="MPNRRVHTVILGGGQGKRLFPLTRDRAKPAVPLGGKYRLIDVPL
                     SNAINSGFRDIAVLTQFNSASLNNHISLTYRFDSFARGQVELIAAQQTDEGGEWFEGT
                     ADAVRKHLRRLGTRDYDHVLILAGDHLYRMDYREMFDAHVQSSADVTVGVLPVGVEEC
                     SGFGILLTGSDGQIRAFREKPKTEEELGPLAPSKELRDRWGLQPGQYLASMGVYVFRM
                     ETLREALASPANMDFGKDIIPGHIGSHKVQAFPFRGYWRDIGTIRSFYEANIALTDDD
                     PPFHFHHPNAPIYTRPRFLPSSSLIDVKVDHCRLADGVRIYGAEVTRSVIGLRARIEK
                     GGRIVESILMGADEFEDEEIAVSALNEGLPPVGIGANCVIERAIVDKNARIGAGCVLR
                     GDPSRPDQDGDGWCVRDGIVIVPKNAVLRPGTVV"
     gene            3538..>4299
                     /locus_tag="P5164_16280"
     CDS             3538..>4299
                     /locus_tag="P5164_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRY45497.1"
                     /translation="MIDGILGAKRPHPGGTISCRVPRGTSEAERQESRIRTLGPSRLR
                     HLAGSAVLVLAGASAACTSVPPAGPAPSASPAPRAAVRPAAPPLLEDWRDAVLYFVVL
                     DRFADGDEASNVDVDPAKKGYFHGGDAKGLTAQLDEIASLGVNALWITPIVRNTPGFV
                     TGAGFPDWAYHGYWADDFLSLDPRFGSENDFKFLVAACHARGIRVLLDVVYNHAGYES
                     KYTKDPKTKGWFRTNEKGNCGTDDITSCVAGLPDFR"
BASE COUNT          588 a         1584 c         1510 g          617 t
ORIGIN      
        1 gcatgaaggc gtgctcgaag acgtccatga tcatcaggag cgtgccgccg gccgggtgcc
       61 cgccgtcgtg ctcgccgatc cacatgttca tcagctggcc ggagacgggg tcctggtaga
      121 gcgccgtcca gccgatgccg cgcatcgcgc cgaccgcccg gaagttcttc tcccacgctt
      181 cgaccgaccc gaagtcggcc gcgagcttct tcgcgagcgc cgagcctccg tcgagggccg
      241 cgcccccgtt cttcatgttg ccgaagtagt actcgtggag ccgcatgccg ttccactccc
      301 agccgaggcg gcgcttcatc tcggcgaagg cgggagtggc ggcctttccg tcggccgcga
      361 gggcggcgag ctcgtcgttc accttgttcg tgttcgtgac gtagccctgg tagagcgtga
      421 agtggttctt catgagggcg tcggagaagc ccggggtgcc gaggaggtgg tcgtagctct
      481 tcgccgtgta ggccatggat cttcctttct cggggatgtc tgacggaacg tctccgattc
      541 tacgttgtgc gtcggcagac ggattcccgc ccgggcccgc ctcagcctcc gagctccggc
      601 cgaaaggccc ggtcgagccg ggcgagccgc gcgaggaaaa gggcgtcggc acggcttcgc
      661 ctgcgccccg cgagctccgc gagcgcccgg agcgccccgg cgtgctcctc gatcctccgc
      721 tcggcgtaat cccgcgccgt cccgccgtcg accaggaacg gccagtcgga ggcctggagg
      781 aggagcgcct gccggagcgc cgccggcagg agccccggat cccgcgcctc gatgcgccgc
      841 agctgcgctt ccgcctcttc cacgctctgc cagaacggct tcgtctccgc gtcgtcccag
      901 acgcggaagt cgccgccccg cccccacgag ccggcccccg gctcgacctc gggcgcgtcg
      961 gctcccgctt cccgcaccgc ctctccggcg gtctccgccc ggaccggacc gtcccgccgc
     1021 gccagctcgc ggaagaccgc cccgaggaac gcgatcccct cgaaccacca gtggccgaag
     1081 agctcgaagt cgaacatcgc gacgatgacg cgggccccac gctccgcccg cgccgtctcc
     1141 tcgaggaggt cgcagaagtg ggtcgcgtgg gccacgacgc gctcgcgcgt ctcctcgggc
     1201 tcgtagagcg ccttggcgtc gagcggtccc cgcgcgtccg tcaccgacca gtagcggagg
     1261 cccccgcgcg ccgccttccg gtggaactcg aggtaggccg ggtctcccgg gtagccgacc
     1321 tcgcccgacc agacctgctc ggagctgcgc gggtcgcgcg cgaggagggc gaggctcccg
     1381 gcgtcggtcc ggacgcggtg gactcggtag ggcgaacgcg ccgccggccc cccccctgcg
     1441 gccggccagc ggcgcccggc gctgccgccg gcggccgttc ccgccgacgg cccggccacg
     1501 aggtgcgtgt cgacgaacgt gtaggcgagt ccccgcgacg cgaggagaga cgaggtcgag
     1561 cggcgcgcga cccgccggcc gtacccgggc gtcgcgagcg ttccggcggg cgcggttgcg
     1621 cactcgggga gccagaagcc ggccgcggcc cggccgaagt gccgccggtg ggccgcgagg
     1681 ccgacgtcga cctgccgctc ggcggccgcg tcggagggaa ggagcggaag gaagccgtgc
     1741 gtcgccgccg aagccatcgc ctcgatccgc cccgcctccg ccagccgggc gaacggggcg
     1801 acgacgtccc cgccgaaagc gtcgaagagc gcgagcccct cctcgtagtg ggcgcgccag
     1861 gcgcgcgcga gcggctcggc gacgtggccg tcccgtgcga actcgcgcgc gtcccgcccc
     1921 gccgcccgga gtcggtgcgt cacgtagcgg cggaacgagc gcgggaagcg cgggtcgcgc
     1981 agctgctcgc agaggatcgg cgtcagcccg accgtcgcct tccacgggac cccctcggcc
     2041 tccagcgccc gcgccgccgc gacgaacggc aggtaggtct ccgccgccgc ctcgtgcagc
     2101 cagtccgctc cgtgcggcca gaccccgtgg ccgaggaccg ccggcatgtg gccgtggagg
     2161 acgaggacga aggagacggg gggcacgggc tcctccgcgg ggacacgaag ggggccgggg
     2221 aagggatcgc ccctcccccg gccctcggtt cagacgacgg tcccgggccg caggacggcg
     2281 ttcttcggga cgatgacgat cccgtcgcgg acgcaccagc cgtccccgtc ctggtcgggg
     2341 cggctcgggt cgccgcggag gacgcacccc gccccgatcc gggcgttctt gtcgacgatg
     2401 gcccgctcga tgacgcagtt ggccccgatc ccgaccggag ggagcccctc gttgagggcg
     2461 ctgaccgcga tctcttcgtc ctcgaactcg tccgccccca tcaggatcga ctcgacgatc
     2521 cggcccccct tctcgatccg ggcgcggagg ccgatgacgc tccgggtcac ctcggcgccg
     2581 tagatcctca ccccgtccgc caggcggcag tggtccacct tcacgtcgat gagcgaggag
     2641 gaggggagga agcgcgggcg cgtgtagatc ggcgcgttcg ggtggtggaa gtggaacggc
     2701 gggtcgtcgt ccgtcagggc gatgttcgcc tcgtagaagc tccggatcgt cccgatgtcg
     2761 cgccagtagc cccggaacgg gaacgcctgc accttgtggc tcccgatgtg ccccgggatg
     2821 atgtccttgc cgaagtccat gttcgcgggg ctcgcgaggg cctcgcgaag cgtctccatc
     2881 cggaagacgt agacccccat gctcgcgagg tactgcccgg gctggaggcc ccaccggtcg
     2941 cgcagctcct tggagggggc gagcgggccg agctcctctt ccgtcttcgg cttctcgcgg
     3001 aaggctcgga tctggccgtc ggagccggtc aggaggatcc cgaaccccga gcattcctcg
     3061 acgccgaccg ggaggacgcc gaccgtcacg tcggcgctcg actggacgtg ggcgtcgaac
     3121 atctcccggt agtccatccg gtagaggtgg tcccccgcca ggatcaggac gtggtcgtag
     3181 tcccgcgtcc cgagccgccg gaggtgcttc ctcaccgcgt ccgccgtccc ctcgaaccac
     3241 tcccccccct cgtcggtctg ctgcgccgcg atcagctcca cctggccccg ggcgaagctg
     3301 tcgaagcggt acgtcaggga gatgtggttg ttcagcgagg cggagttgaa ctgcgtcagg
     3361 accgcgatgt cccggaagcc cgagttgatc gcgttcgaga gcgggacgtc gatcagccgg
     3421 tacttcccgc cgagcgggac ggccggcttg gcgcggtcgc gggtaagggg gaagaggcgc
     3481 ttgccctggc ccccgccgag gatcacggtg tggacgcgtc ggttcggcac ttctcgcatg
     3541 atcgatggaa ttctaggcgc gaaacggccg catcccggtg ggacgatttc ctgtagagtt
     3601 ccgcgaggaa catcggaagc cgaacgacag gagagccgta ttcgcacgct cggtccgtcc
     3661 cgactccgtc acctcgcggg gagcgccgtc ctcgtcctcg cgggtgcctc ggccgcctgc
     3721 acctcggtcc cgcccgcggg gccggcgccc tccgcatcgc cggccccgcg ggccgccgtc
     3781 cggccggcgg cgcccccgct cctcgaggac tggcgcgacg ccgtcctcta cttcgtcgtc
     3841 ctcgaccgtt tcgcggacgg cgacgaggcg agcaacgtcg acgtcgaccc ggcgaagaag
     3901 gggtactttc acggcggcga cgcgaagggg ctgacggctc agctcgacga gatcgcctcc
     3961 ctcggcgtga acgctctctg gatcacgccg atcgtgagga acacccccgg cttcgtcacc
     4021 ggggccggct tccccgactg ggcctaccac ggctactggg ccgacgactt cctctccctc
     4081 gacccccgtt tcggcagtga aaacgacttc aagttcctcg tggccgcctg ccacgcgcgc
     4141 ggcatccgcg tcctcctcga cgtcgtctac aaccacgccg gctacgagtc gaagtacacg
     4201 aaggacccga agacgaaggg gtggttccgg acgaacgaga aggggaactg cggaacggac
     4261 gacatcacgt cgtgcgtcgc cggcctcccc gacttcaga
//
LOCUS       DAOTEZ010000383         4280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_15133_length_4280_cov_5.134201, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000383 DAOTEZ010000000
VERSION     DAOTEZ010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15133_length_4280_cov_5.134201"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="P5164_16285"
     CDS             complement(<1..335)
                     /locus_tag="P5164_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRY45498.1"
                     /translation="MSMPGTGRSLIEELQERAKELQCLYRVHAICARAEAPLDELLRE
                     VASVLPAGWEHPAETVVRIVVGSSVFVSSEAEPTPWVQAAPIRVEGQEVGRLEVSYRE
                     PFPVSDEGP"
     gene            complement(492..1775)
                     /locus_tag="P5164_16290"
     CDS             complement(492..1775)
                     /locus_tag="P5164_16290"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRY45499.1"
                     /translation="MKTPFNPFEMAQAQFDKAADVLGLDPATRDLLRFPLREYHVAIP
                     VRMDDGSVKVFRGFRVQHNDSRGPCKGGIRFHPHETADTVRALAMWMTWKCAVVDIPL
                     GGGKGGVICDPHHLSAREQEGICRGWIRQVAKNVGPVNDVPAPDVMTTPQHMLWMLDE
                     YETLAGGKYPGFITGKPVGSGGSLGRTEATGYGVVYTIREALKQLGIDITKTKASVQG
                     FGNVAQYAIKLYTQLGGKVVCCSSWDQADQTSYSFRRLSGVDADELLGITDRFGGIDK
                     DKAKGLGYEVLPGDAWIEQDVEILIPAALENQVTGETVKKIKPSVKVIAEGANGPTTP
                     EADKVIQERGIYVIPDFLANAGGVTCSYFEQVQCNMNFFWEKDEVLQKLDSKMTAAYH
                     AVHELAKKRNLYMRDAAYVIAVSRVAKACKDRGWA"
     gene            2010..>4280
                     /gene="hrpB"
                     /locus_tag="P5164_16295"
     CDS             2010..>4280
                     /gene="hrpB"
                     /locus_tag="P5164_16295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HRY45500.1"
                     /translation="MKTTLPIDPFLPEIAATLRRRRTLVLLAPPGAGKTTRVPRALLD
                     AGLLDAGALWVLQPRRIAARAAVRRLAAELGEEVGGTVGYQVRDEGAHSERTRILVVT
                     EGILARRLLDDPALPGIAGVMIDEFHERSRHADLALAMLREVREALREDLSLLVASAT
                     LDPEPVARFLADGRGPAPVLSVPGRPFPVEVRHAAGPDPRPLEVRVASAVATALAEGP
                     PGDVLVFLPGAAEIRRARDSIASLDARLDLRILHGDLPSAAQDAALASPADGRRRVVL
                     ATNVAETSLTIEGVCAVVDSGLARTLRFDPRSGLDRLLLGRIPRASADQRAGRAGRLG
                     PGLAIRLFTEHEERGLRPFEEPELLRADLSAPLLDILLWSPRDPSRFGWFEAPPASSV
                     ERALRLLRRLGAVEERSFQPTPLGRRMARLPLHPRLGALVLAGHAAGLAREAATLAAL
                     VEERDVLAGSRAFGRAAEAPEHDSDLLLRLELLEEAERAGLSRPRLESFGLDPGAAHA
                     VLRLRDTLLRRLPSAPRPPRAREEDLLRLVLHGWPDRVARRRRGRPSEAVLAGGTPLA
                     FDPGCAVRKADLVAVVETVAGTNGPDRIRLASAVERSWLDALPGAGVLSETVRSWDAA
                     AGRVVATQRVVFDGLVLEEAEISAPPDAETARLVLATVLVKKSGSDQSGTVYAMRDIG
                     EDRARFRDSALANSQTMVDVWEVYYETADLGRRVCCFCPQFPDLVLSDRPWTWVALIG
                     VESEPPVREWPFVQFRN"
BASE COUNT          512 a         1605 c         1505 g          658 t
ORIGIN      
        1 aacggccctt cgtccgagac cgggaacggc tcgcggtagg agacctcgag gcgtccgacc
       61 tcctggccct cgacccggat cggcgccgcc tgcacccagg gggtcggctc cgcctcggac
      121 gagacgaaga cggaggagcc gacgacgatc ctcacgacgg tctcggccgg gtgctcccag
      181 cccgccggca ggacgctcgc gacctcccgg aggagctcgt cgagcggagc ctccgcccgc
      241 gcgcagatcg cgtggacccg gtagagacac tggagctcct tcgcccgctc ctggagctcc
      301 tcgatcagcg aacgacccgt accgggcatc gacacctcct gcggctcgcc gtgcggcgcg
      361 agagtgccac agggccggtc gttcagggag cggtcagcgg gaacgccgcc cgaggcggga
      421 gaagcgggcg gcccggtcgc ggggaagggc cgggggagcc cctctcggag cccccccggc
      481 cggggaaccg atcaggccca gccgcggtcc ttgcaggcct tggccacgcg ggagaccgcg
      541 atgacgtagg ccgcgtcgcg catgtagagg ttgcgcttct tcgcgagctc gtggacggcg
      601 tggtacgccg ccgtcatctt cgagtcgagc ttctggagga cctcgtcctt ctcccagaag
      661 aagttcatgt tgcactgcac ctgctcgaag tagctgcagg tgacgccgcc ggcgttcgcc
      721 aggaagtcgg ggatgacgta gatgccgcgc tcctggatca ccttgtcggc ctcgggcgtc
      781 gtcggcccgt tggctccctc ggcgatgacc ttcacgctcg gcttgatctt cttgaccgtc
      841 tcgcccgtca cctggttctc gagggcggcc gggatgagga tctcgacgtc ctgctcgatc
      901 caggcgtcgc cggggaggac ctcgtagccg aggcccttcg ccttgtcctt gtcgatcccg
      961 ccgaagcggt cggtgatgcc gaggagctcg tcggcgtcga cgccggagag ccggcggaag
     1021 gagtacgagg tctggtcggc ctggtcccac gacgagcagc agacgacctt gccgccgagc
     1081 tgcgtgtaga gcttgatcgc gtactgcgcg acgttgccga agccctggac ggaggccttc
     1141 gtcttcgtga tgtcgatccc gagctgcttc agcgcctcgc ggatcgtgta gacgacgccg
     1201 tagcccgtcg cctcggtccg gccgagcgag ccgcccgagc cgaccggctt gccggtgatg
     1261 aagccggggt acttgccgcc cgcgagcgtc tcgtactcgt cgagcatcca gagcatgtgc
     1321 tgcggggtgg tcatgacgtc gggcgccggg acgtcgttga cggggccgac gttcttggcg
     1381 acctggcgga tccagccgcg gcagatcccc tcctgctcgc gcgcgctcag gtggtgcggg
     1441 tcgcagatga cgccgccctt gccgccgccg agcgggatgt cgacgaccgc gcacttccag
     1501 gtcatccaca tcgcgagggc gcggaccgtg tcggccgtct cgtgcgggtg gaagcggatg
     1561 ccgcccttgc aggggccgcg ggagtcgttg tgctggacgc ggaacccgcg gaagaccttc
     1621 acgctcccgt cgtccatccg gaccgggatc gcgacgtggt actcgcggag ggggaagcga
     1681 aggaggtcgc gcgtcgccgg gtcgaggccg aggacgtcgg ccgccttgtc gaactgcgcc
     1741 tgggccatct cgaaggggtt gaagggagtc ttcatggaac gacgaatctc ctctcgtggg
     1801 tggtgggcgg tttgtggtgg gccgggtctt ctctgttcgc gagcggaccc ggaatacgcc
     1861 tccgctccgc gcctgggact cgggagaacg gagccgcgaa ccggcgggcc atcaacgggt
     1921 gattctagca ccgcggaggg ctttccgagc cgcctgaagc ccagctccgg cgcggtggcg
     1981 gccggccccc cgtatcctgt ccgcccctgg tgaagacgac gcttccgatc gacccgttcc
     2041 ttcccgagat cgccgcgacg ctccggcggc gccgcacgct cgtcctcctc gccccccccg
     2101 gcgccggaaa gacgacccgc gtgccgcgag cgcttctcga cgcgggcctc ctcgacgcgg
     2161 gcgccctctg ggtcctccag cctcgccgga tcgcggcgcg ggccgccgtg cggcgcctcg
     2221 ccgccgagct gggagaagag gtcggaggca ccgtcggata tcaggtccgc gacgaagggg
     2281 cgcattcgga gaggacccgc atcctcgtcg tcaccgaggg gatcctcgcg cggcgcctcc
     2341 tcgacgaccc cgcgctcccc gggatcgccg gcgtgatgat cgacgagttc cacgagcgga
     2401 gccgccacgc cgacctcgcc ctcgcgatgc tccgggaggt ccgggaggcg cttcgcgaag
     2461 acctgtctct cctcgtcgcc tccgcgacgc tcgatccgga gcccgtcgcg cggttcctcg
     2521 ccgacgggcg gggaccggcg ccggtcctct ccgtgccggg ccgtcccttc ccggtcgagg
     2581 tccggcacgc ggccggtccc gatccgcgcc ccctcgaggt ccgtgtcgcc tccgccgtcg
     2641 cgacggccct cgcggagggg ccgccggggg acgtcctcgt cttcctccct ggagcggccg
     2701 agatccgccg ggcgcgcgat tcgatcgcct cgctcgacgc gcgcctcgac ctccggatcc
     2761 tccacgggga cctcccttcc gccgcccagg acgcggcgct cgcatctccc gccgacggac
     2821 gccgtcgcgt cgtcctcgcg acgaacgtcg ccgagacctc cctgacgatc gagggggtct
     2881 gcgccgtcgt cgactccggc ctcgcgcgga cgctccggtt cgacccgcgc tccggcctcg
     2941 accgcctcct cctcggccgg atcccccgcg cgagcgccga ccagcgggcc ggtcgcgccg
     3001 ggcgcctcgg ccccggcctc gcgatccggc tgttcacaga gcacgaggag cgcgggctgc
     3061 gtccgttcga ggaaccggag ctcctccggg ccgacctctc cgcgcctctg ctcgacatcc
     3121 tcctctggtc gccccgcgac ccgtctcgct tcggctggtt cgaggcgccg cccgcctcct
     3181 ccgtcgagag ggcgctccgc ctcctccgcc ggctgggggc cgtcgaggag cggtcgttcc
     3241 agccgacccc gctcggccgc cggatggccc gcctcccgct tcacccgcgt ctcggcgccc
     3301 tcgttctcgc aggccatgcg gccggcctcg cgcgcgaggc ggcgacgctc gcggcgctcg
     3361 tcgaggagcg cgacgtcctc gccgggagcc gcgccttcgg ccgggcggcc gaggcgccgg
     3421 agcacgactc cgacctcctt ctccgcctcg agctcctcga ggaggccgag cgggcggggc
     3481 tctcccgccc gcggctcgag tcgttcggcc tcgatccggg cgccgcccac gccgtcctgc
     3541 gcttgaggga cactctcctc cgtcgcctcc cctctgctcc gcggcctccg cgcgcccgcg
     3601 aggaggacct cctccggctc gttctccacg gatggcccga tcgcgtcgca cggaggaggc
     3661 gaggccgccc atccgaggcg gtcctcgccg gcgggacacc gctcgccttc gacccgggct
     3721 gtgccgttcg gaaggccgat ctcgtcgccg tcgtcgagac cgtcgcgggg acgaacggcc
     3781 ccgaccggat ccgcctcgcg agcgccgtgg aacgctcgtg gctcgacgcg ctgcccggcg
     3841 cgggcgtcct ctcggagacg gtccgctcct gggacgctgc cgccggccgc gtcgtcgcga
     3901 cgcagagggt cgtcttcgac ggtctcgtcc tcgaggaggc cgagatctcc gcgccccccg
     3961 acgcggagac cgcgcggctc gtgctcgcga cggtgctcgt caaaaagtcc ggctcggatc
     4021 agagcggcac ggtctacgcg atgcgcgaca ttggcgagga ccgggcgcgg ttccgcgact
     4081 cggcgctggc caactcgcag accatggtgg acgtctggga agtctactac gagaccgccg
     4141 acctcgggcg gcgcgtctgc tgcttctgcc cgcagtttcc ggatctcgtg ctgtccgacc
     4201 gcccctggac ctgggtcgcg ctgatcggcg tggagtccga accgccggtg cgtgaatggc
     4261 cgttcgtgca gttccgcaac
//
LOCUS       DAOTEZ010000384         4271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_15161_length_4271_cov_6.832780, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000384 DAOTEZ010000000
VERSION     DAOTEZ010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15161_length_4271_cov_6.832780"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..159)
                     /locus_tag="P5164_16300"
     CDS             complement(<1..159)
                     /locus_tag="P5164_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRY45501.1"
                     /translation="MPVINSQIPEFKVQAYHNGAFKEVSNKDLAGKWSVFFFYPADFT
                     FVCPTELGD"
     gene            389..465
                     /locus_tag="P5164_16305"
     tRNA            389..465
                     /locus_tag="P5164_16305"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:423..425,aa:Met,seq:cat)
     gene            944..1291
                     /locus_tag="P5164_16310"
     CDS             944..1291
                     /locus_tag="P5164_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HRY45502.1"
                     /translation="MPQYLVAGYLPDDFDTSQMDEAVGREIHALNEEMIAAGVRKFAC
                     GLGAAKSLRSRADGGVLVTDGPYLETKEHIGGFWILECADMDEAVAWARKGVAAMRGQ
                     VEVREIFFVPTPE"
     gene            1383..1766
                     /locus_tag="P5164_16315"
     CDS             1383..1766
                     /locus_tag="P5164_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HRY45503.1"
                     /translation="MPQYLVAIQHPEDYDATQEGEAMIRDIAALNEEMDAVGVRFFAG
                     GLESARGARSLRRQPGGGIAITDGPYLEAKEHVGGFWILECADMDEALAWGRKAVVAC
                     RVPVEVRAFLRTRPKRESPGRGGSE"
     gene            1794..3062
                     /locus_tag="P5164_16320"
     CDS             1794..3062
                     /locus_tag="P5164_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRY45504.1"
                     /translation="MSDVHQAIEAVWRLESTRLIAAIARVVRDVGLAEELAQEALVTA
                     LEVWPEEGIPEKPGAWLMTAAKRRAIDSLRRGRMLERKHGEIGRELEEQQQRLGDALD
                     HARDEVIDYDVLRLVFTACHPVLAVEGRIALTLRLVGGLTTAEIARAFLVPEKTMGQR
                     ITRAKRTLSEAHVPFETPRGDELRLRVESVLLVVYLIFNEGYTATAGEEWMRAVLADE
                     ALRLGRMLVRLLPGEPEVHALVALMELQASRNAARRGPNGEAVLLSDQDRSQWDRAQI
                     RRGLAALERAQRLGGGARPYALQAALAACHARARAAEETDWERIVLLYDALLQVLPSP
                     VVALNRAVAVGMARGPAAGLDALDAATALAGESAFAGYHLFPSVRGDLLMKLGRLSEA
                     RDEIRRALRMTKNVRERELLEKRLRQWETA"
     gene            3075..3998
                     /locus_tag="P5164_16325"
     CDS             3075..3998
                     /locus_tag="P5164_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45505.1"
                     /translation="MPVFHAGRDRVRLEAPEPAVPELAASLWPEVPDADARADELRLR
                     IEVSDGAVEGDPEEALLWDVSGDETRLLAPGIELTVRPRARRASATVARALVSSRPDL
                     VTRLLLEAPVTAMKMVMMQLLHAGAVVGPAGAVVVRGAAGAGKSTLVAACRAAGLGFL
                     GDESLVVDRDDADRLEATLRDLVLTPESARLLGLEGASVPAFSGGERKRRVPLPEPLV
                     PADRLARRVATLLLGPREPGPARLVPVAGAAFLEAFAAGEIPQERMYGGDPAAIARAW
                     AGRETYFLTGAVDLGGAVDQIRDLVHGFGAR"
     gene            4022..>4271
                     /locus_tag="P5164_16330"
     CDS             4022..>4271
                     /locus_tag="P5164_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HRY45506.1"
                     /translation="MSAAGPPVLLAHSCFLTHDPKQVRKMRPYSPLATLLAASVVRRS
                     GREVALFDAMLSAGEEEFEAVLARVAPRVVAILEDNFNF"
BASE COUNT          608 a         1536 c         1514 g          613 t
ORIGIN      
        1 gtcgccgagc tcggtcgggc agacgaacgt gaagtcggcc gggtagaaga agaagaccga
       61 ccacttgccg gcgaggtcct tgttcgagac ctccttgaag gcgccgttgt ggtaggcctg
      121 aaccttgaat tcgggaatct ggctgttgat gacgggcatg ctcgttctcc ttgcgttctc
      181 tgcgggcgag tcggcccgct ctttagactt tttctaattt acgacaggat cgaagaaaag
      241 gcaagccccg ttcgaccgat tcgttttccc ggctcgaggc ggaccccggc ccgggaccgc
      301 tcctcgcctc agtcccttca cgaagcacct gcgcccgcga cttcgcttga cttccctcca
      361 tacccccggt atcttccccc tcccctgacg cggggtggag cagcctggta gctcgttggg
      421 ctcataaccc aaaggtcgca agttcaaatc ttgcccccgc aaccaaccat aagatccgca
      481 gaatcaagcg gttagaaggc ccggcggaaa cgccgggctc ttcgttttcg ggagcctccc
      541 cgggcctcgg gctcgcccgg ggccccgcga gtcgcacgga ggcgcgcgag cggacgggga
      601 acgaacccgc ccgcggcggg cccggcgcgg ggcgccgctc gcacctcgct gccccgcccc
      661 gcgaccggga cggaacccgt cccctccgtc gggcccacgt gcgcgccggg acgagctcag
      721 gccgagatca cgtggcggca acgaggtccg ctccgttccg gcgggaccgt gccgaggtcg
      781 cgggatggga ccgggcggct ccgacgacct ctggcggtcg aatcgggcat cgcccgcccg
      841 gcaggaatgg attctctcgt cggctcgtag aattccgccc tcgccgttcg tcgttggagt
      901 gaaggccggg cagacagcct cgcccgaggg tcaggagaag accatgccgc agtacctcgt
      961 tgccggttac ctccccgacg atttcgacac gtcgcagatg gacgaggcgg tgggccgcga
     1021 gatccacgcg ctcaacgagg agatgatcgc cgccggcgtc cggaagttcg cctgcggcct
     1081 cggggccgcg aaatcgctcc ggtcccgggc cgacggcggg gtcctcgtca ccgacggacc
     1141 gtacctcgag acgaaggagc acatcggggg gttctggatc ctggagtgcg ccgacatgga
     1201 cgaggccgtg gcgtgggcgc gaaagggcgt cgccgccatg cgggggcagg tcgaggtgcg
     1261 ggagatcttc ttcgttccga cgcccgaata gcccgacgcg cgccccgccc gacgacgcag
     1321 aagccgcccg acctcgaggc gggcatccga gagagctgtc gcccgacctg gccggaggaa
     1381 acatgccgca gtacctggtc gccatccagc atcccgagga ctacgacgcg acgcaggagg
     1441 gcgaggcgat gattcgcgac atcgccgcgc tcaacgaaga gatggacgcc gtcggcgtcc
     1501 gcttcttcgc cggcggcctc gaatcggcga gaggcgcccg atcgctgcgc aggcagcccg
     1561 gcggcgggat cgccatcacc gacggcccct acctcgaagc caaggaacac gtcggcggct
     1621 tctggatcct cgagtgcgcc gacatggacg aagccctggc ctggggtcga aaggccgtcg
     1681 tcgcctgccg tgttcccgtc gaggtgcggg ccttcctgag gacccggccg aagcgggaat
     1741 cgcccggccg cggaggctcg gagtgaggaa gcggacccgg tagcctcacg agcatgagcg
     1801 acgtccacca ggcgatcgaa gccgtgtgga ggctcgagtc cacacggctc atcgccgcga
     1861 tcgcccgcgt cgtccgcgac gtcggcctcg ccgaggagct cgcgcaggaa gcgctcgtga
     1921 cggccctcga ggtctggccc gaggagggca tccccgagaa gcccggcgcc tggctgatga
     1981 cggccgcgaa gcggcgggcg atcgattcgc tgcggcgcgg ccgcatgctc gagcggaagc
     2041 acggggagat cggccgcgag ctggaggagc agcagcagcg gctcggagac gccctggacc
     2101 acgcgcggga cgaggtgatc gactacgacg tgctgcggct cgtcttcacc gcgtgccatc
     2161 ccgtgctcgc cgtggaaggg cgcatcgcgc tcacgttgcg cctcgtcggc ggcctcacga
     2221 cggcggagat cgcgcgcgcg ttcctcgtcc cggagaagac catggggcag cggatcactc
     2281 gcgccaagag gacgctctcg gaggcccacg tccccttcga gacgccgcgc ggggacgagc
     2341 tgcgcctccg ggtcgagtcg gtgctcctgg tcgtctacct gattttcaac gagggctaca
     2401 ccgcgacggc gggcgaggag tggatgcgcg cggtcctcgc cgatgaggcg ctgcgcctgg
     2461 gccgcatgct cgtccgcctc ctccccggcg agccggaggt ccacgcgctc gtggccctga
     2521 tggagctgca ggcctcgcgc aacgcggcac gccggggccc gaacggcgag gccgtcctcc
     2581 tctcggatca ggaccgctcc cagtgggacc gcgcgcagat ccggcgaggg ctcgccgccc
     2641 tcgagagggc gcagaggctc ggcggcggcg cgcggcccta cgctctgcag gccgccctcg
     2701 ccgcctgcca cgcgcgcgcc cgcgccgccg aggagacgga ctgggaacgc atcgtccttc
     2761 tctacgacgc cctgctgcag gtgcttccct ctcccgtcgt ggcgctcaac cgcgcggtcg
     2821 ccgtcggcat ggcccgggga ccggcggcag gtctcgatgc gctcgacgcg gcaaccgctc
     2881 tcgccggcga gtccgcgttc gccggctacc acctcttccc cagcgtccgc ggggaccttc
     2941 tgatgaagct gggccggctc tccgaggcac gcgacgagat ccggcgcgct ctcaggatga
     3001 cgaagaacgt gcgggaacga gagctcctgg agaagaggct gaggcagtgg gagacggcct
     3061 aagatcacgc ctccatgccc gtcttccacg ctggccgcga tcgcgtccgg ctcgaggcgc
     3121 cggagcccgc cgttcccgag ctcgccgcgt ccctctggcc tgaggtcccc gacgccgacg
     3181 cccgcgccga cgagctccgc ctccggatcg aggtgagcga cggtgcggtc gagggcgacc
     3241 cggaggaagc gctcctctgg gacgtgagcg gcgacgagac gcgcctcctc gcgccgggaa
     3301 tcgagctaac ggtccggccg cgggcgcgtc gcgcctcggc gaccgtggct cgcgcgctcg
     3361 tctcctcgcg cccggacctc gtgacacggc tccttctcga ggcgcccgtg accgcgatga
     3421 agatggtgat gatgcagctc ctccacgcag gcgcggtcgt cggccccgcg ggcgccgtcg
     3481 tcgtgcgcgg agcggcgggg gcgggcaagt cgactctcgt ggccgcctgc cgcgcggccg
     3541 gcctcggatt cctcggcgac gagagcctcg tcgtcgaccg ggacgacgcc gaccgcctcg
     3601 aggcgacgct ccgcgacctc gtcctcacgc cggagtccgc ccgcctgctc ggcctcgaag
     3661 gggcgtcggt cccggccttc tcgggcggcg agcggaagcg ccgcgtgccg ctgccggagc
     3721 cgctcgtgcc ggccgaccgc ctcgcgcgcc gcgtggcgac gctgctcctc gggccgcgcg
     3781 agccgggccc cgcgcggctc gtcccggtcg cgggagccgc gttcctggag gcgttcgccg
     3841 ccggcgagat cccgcaggag cggatgtacg ggggagatcc cgcggcgatc gcccgggcgt
     3901 gggccggccg ggagacgtac tttctgacgg gagccgtcga cctcgggggc gccgtcgacc
     3961 agatccgtga tctggtgcac gggttcggcg cccgctgaat tcctagactg cgtctcaaag
     4021 aatgagcgca gccggacccc ccgtcctcct cgcccactcc tgcttcctca cgcacgaccc
     4081 gaagcaggtg aggaagatgc gtccctactc gccgctcgcg acgctcctcg ccgcctcggt
     4141 cgtccggcgc tcggggcggg aggtcgccct cttcgacgcg atgctctcgg cgggcgagga
     4201 ggagttcgag gcggtcctcg cgcgcgtcgc cccgcgggtc gtcgcgatcc tcgaggacaa
     4261 cttcaacttc c
//
LOCUS       DAOTEZ010000385         4259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_15225_length_4259_cov_5.611798, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000385 DAOTEZ010000000
VERSION     DAOTEZ010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15225_length_4259_cov_5.611798"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="P5164_16335"
     CDS             complement(<1..693)
                     /locus_tag="P5164_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45507.1"
                     /translation="MRRRAAGAALALFPALLAAGCGLPERVRGLVARPTPTALPRPTP
                     VPAPPLELSFLGIREDRVRGGAAGPAGTTIEVALVGTKRGDVETARVVVETAFDDLGT
                     SLVPAGAEDAAHEPPRGDDAAAPVVLSVPLKLASRAASSLREVSGSIELYVPDADPGA
                     VVTVPRFRSRAGSTLASPSLAEHAVAIAIATAQEAEALPADPDDLVLRVADPNGRVEG
                     FYFVDPEGTAWAT"
     gene            complement(690..1457)
                     /locus_tag="P5164_16340"
     CDS             complement(690..1457)
                     /locus_tag="P5164_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45508.1"
                     /translation="MKRQDAVSPVLLATAALALAAAAARVDAQGTFRLVVDAVAESRH
                     RSASNEAEGRLTLQPKLEGDGLADAKAFRLRLATAVDATGRDVLPEEPEPPRWEERPS
                     GPDLWIVLASPARRAAWVTVSGTIDLWIPSRDPGAEATVPRALARPGAVLAAPGLRSA
                     GLTLRLAGRGESSPTVVSLRGRSADVERVRSVRVLRADGTELSTSGLQVTTAGEKGSL
                     ELLLAEPPPADATLVLGLLTKRSVVAVPFELVEVALP"
     gene            complement(1643..3070)
                     /locus_tag="P5164_16345"
     CDS             complement(1643..3070)
                     /locus_tag="P5164_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRY45509.1"
                     /translation="MKKTSNLAAAVALLGAAALLAPAARAAEPAPLPKELPPYGADAP
                     LPVPAIAKSTLPNGLTAWIVKRPGFPKVSAVLAVRGGTAADPAGMEGVAGLLAATMKE
                     GTARRSSRRIAEELQAVGGDLSVAASADAFYLRGSALASGLPALLDLLADVARNPAFP
                     PGEVDLAKENALQELEAQEATPEYLAARAFAKAVYGSHPYHVVSPTRETVGKVAPALL
                     RAEHARRFRPDAALLVLAGDLDEAAAKAAVAKAFGGWAATGSPVPSTPPSPPARGREI
                     FLVDRPGSVQSTVVAGRPGPDVSDPDWYDVLVANTIYADAFGSRLVRNIREEKGYTYS
                     PSGDFATRRAGSLLEMRADVRNEVTAATLLEVFYELDRMAATTPTPDELAGAKRYQAG
                     LYLLRNQVQEAVSGTLARNWVNGLPPESLGEFVTKVNAVTADGVRAAGRKHFLSSSQT
                     VVVVGDAKEARAQLELFGKVVDVNP"
     gene            complement(3081..>4259)
                     /locus_tag="P5164_16350"
     CDS             complement(3081..>4259)
                     /locus_tag="P5164_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRY45510.1"
                     /translation="RTGFAHLFEHMMFQGTPNAPKGTYDRVIEGGGGINNGSTRYDYT
                     NYIVSAPVSALDAILWLEADRMKMLDFSEKNLQNQKDVVKEEIRVNVKNRPYGLFFWT
                     DVAGAAFTKWENAHDGYGSFADLDAASSADVKAFFESFYGPNNAVLAIVGDVAAEEAF
                     AKVEKYFGSIPARATPPKPDVDEEPNSKERLVEQTDPLANVPAVAVGWKLPSRGTPDD
                     VPVAVLADLLAGGEASRLYQGLVKGKELLLGIEGGVNWPLGDPWTYEGPTLLTLFGLY
                     KPDTTAKAVVAAIDGEIAKVARGDVGVAELERTKTKMVSDLYAQLEMPMDRAVALSLA
                     QLFTGDAASVNEIPRKLSAVTRADLARVASTYLAARNRTVVDRRPAPQKAEPPAEAGK
                     "
BASE COUNT          559 a         1496 c         1638 g          566 t
ORIGIN      
        1 ggtcgcccag gccgtcccct cgggatcgac gaagtagaag ccctcgacgc gtccgttcgg
       61 gtcggcgacg cgcaggacga ggtcgtccgg atcggccgga agcgcctcgg cctcctgagc
      121 cgtcgcgatc gcgattgcga cggcgtgctc ggcgagcgag ggagacgcca gggtcgaccc
      181 ggcccgggaa cggaaacggg ggacggtgac gacggcgccg ggatcggcat cgggcacgta
      241 gagctcgatc gacccagaga cctcccggag cgacgacgcg gcccgggacg cgagcttcaa
      301 cgggaccgag aggacgaccg gcgccgccgc gtcgtccccg cgcgggggct cgtgcgccgc
      361 gtcctcggcg cccgccggga cgaggctcgt cccgaggtcg tcgaaggcgg tctcgacgac
      421 gacgcgcgcc gtctcgacgt cgcccctctt cgtccccacg agggcgacct cgatcgtcgt
      481 cccggccggt ccggcggccc cgccccgcac ccggtcctcg cggatgccga ggaagctcag
      541 ctcgagcgga ggcgccggga ccggcgtcgg ccgcgggaga gcggtcggcg tcggcctcgc
      601 gacgaggccc cgcacgcgtt cgggaagtcc gcacccggcc gcgaggagcg cggggaagag
      661 ggcgagggcg gcgcccgcgg ctcgcctcct cacggtaggg cgacctccac gagctcgaac
      721 ggaacggcca ccacggagcg cttcgtgaga aggccgagga ccagcgtcgc gtcggcgggt
      781 ggcggctccg cgaggaggag ctcgagcgac cccttctctc ccgccgtcgt cacctggagg
      841 cccgaggtcg agagctccgt cccgtcggcg cgaaggacgc gaacgctgcg gaccctctcg
      901 acgtccgcgg agcggccgcg cagggagacg accgtcggag agctctcccc gcgccccgcc
      961 agccggagcg tgaggccggc ggagcgcagg ccgggcgccg cgaggacggc ccccggccgc
     1021 gcgagggccc tcgggaccgt cgcctcggcg cccgggtccc gcgaggggat ccagaggtcg
     1081 atcgtcccgg agacggtgac ccaggccgct cggcgcgcgg ggctcgcgag gacgatccag
     1141 aggtccgggc cgctcgggcg ctcttcccac cggggcggct cgggctcctc ggggaggacg
     1201 tcgcgccccg tggcgtcgac ggcggtcgca agccggaggc ggaacgcctt cgcgtcggcc
     1261 aggccgtcgc cctcgagctt cggctggagc gtgagccgcc cctcggcctc gttcgaggcg
     1321 gagcgatggc gcgattccgc cacggcatcg accacgagcc ggaacgtccc ctgcgcgtcg
     1381 acgcgcgcgg ccgcggcggc gagggccagc gccgccgtcg cgaggagaac cggggagact
     1441 gcgtcctgtc gcttcacgtc gagctcgttc cgaacagccc ggcaagtgta cgtcgaaggg
     1501 tccttcggcc gctcgctggg cgcccgcttc ccggaaccca cggaagtcgg cgtcccgaaa
     1561 gggctcacgg agggcccggg tgctcgggag gccactggcc ctgcgccggg ccgccccgcg
     1621 cgagcggggc ggccccgggg cttcaaggat tcacgtcgac gaccttcccg aagagctcga
     1681 gctgagcgcg agcctccttc gcgtcgccca cgacgacgac ggtctggctg ctcgagagga
     1741 agtgcttcct ccccgccgcg cggacgccgt cggccgtcac ggcgttcacc ttcgtcacga
     1801 actcgccgag cgactcgggc gggaggccgt tgacccagtt gcgggcgagg gttccggaga
     1861 cggcttcctg gacctggttg cggaggagat agagccccgc ctggtagcgc ttcgcgccgg
     1921 cgagctcgtc cggcgtgggc gtcgtcgccg ccatccggtc gagctcgtag aagacctcga
     1981 gaagcgtcgc ggccgtcacc tcgttgcgga cgtcggcccg catctcgagg aggctcccgg
     2041 cgcggcgggt cgcgaagtcg cccgacgggg agtaggtgta ccccttctcc tcgcggatgt
     2101 tcctcacgag gcggctcccg aaggcgtcgg cgtagatcgt gttcgcgacg aggacgtcgt
     2161 accagtcggg gtcgctcacg tccgggccgg ggcgccccgc gacgacggtc gactggacgg
     2221 agccggggcg gtcgacgagg aagatctcgc gcccgcgcgc cggcggcgac ggaggcgtcg
     2281 aaggcaccgg cgagcccgtc gcggcccatc cgccgaacgc cttcgcgacg gcggccttcg
     2341 ccgccgcctc gtcgaggtcg cccgcgagga cgaggagcgc cgcatccggg cggaagcggc
     2401 gcgcgtgctc ggctcggagg agcgccggcg cgaccttgcc gaccgtctca cgcgtcggcg
     2461 agacgacgtg gtacgggtga gagccgtaga cggccttcgc gaaggccctg gccgcgaggt
     2521 actcgggcgt cgcctcctgc gcctcgagct cctggagggc gttctccttc gcgaggtcga
     2581 cctcgcccgg cgggaaggca gggttgcggg cgacgtcggc gaggaggtcg aggagggccg
     2641 ggaggcctga ggcgagcgcg ctcccgcgca ggtagaaggc gtcggccgag gccgcgacgg
     2701 agaggtcccc gccgacggcc tgaagctcct cggcgatccg ccgggaggaa cgccttgccg
     2761 ttccctcctt catcgtcgca gcgaggaggc ccgcgacgcc ctccatgccg gccgggtcgg
     2821 cggccgtgcc accgcggacg gcgaggaccg ccgagacctt cgggaagccg gggcgcttca
     2881 cgatccaggc ggtgaggccg ttcggaagcg tcgacttcgc gatcgccggg acggggagcg
     2941 gcgcgtcggc cccgtaggga gggagctcct tggggagcgg ggccggctcg gccgcgcggg
     3001 cggcgggggc gaggagcgcg gcggcgccga ggagcgccac tgcggcggcg aggttcgagg
     3061 tcttcttcac ggagcgctcc tcactttccg gcttcggccg gaggctcggc cttctgcggc
     3121 gcgggccggc ggtcgacgac ggtccggttc cgagccgcga ggtacgtgga ggcgacgcgc
     3181 gcgaggtcgg cgcgcgtcac ggcggagagc ttccgcggga tctcgttcac gctcgcggcg
     3241 tcgcccgtga agagctgcgc gagggagagc gcgacggcgc ggtccatcgg catctcgagc
     3301 tgcgcataga ggtcggagac catcttcgtc ttcgtcctct ccagctcggc gaccccgacg
     3361 tcgccgcgcg ccaccttcgc gatctccccg tcgatcgccg cgacgacggc cttcgccgtc
     3421 gtgtcgggct tgtagagacc gaagagggtc agaagcgtgg gcccctcgta ggtccacggg
     3481 tcgccgagcg gccagttcac gcccccctcg atcccgagga ggagctcctt ccccttcacg
     3541 agcccctggt agaggcgcga cgcctcgccg ccggcgagga ggtcggcgag gacggcgacg
     3601 ggaacgtcgt ccggggtgcc gcgggagggg agcttccacc ccaccgccac ggcagggacg
     3661 ttcgcgagcg ggtcggtctg ctcgacgagg cgctccttcg agttgggctc ctcgtcgacg
     3721 tcgggtttcg gaggggtggc gcgcgccggg atcgacccga agtacttctc caccttcgcg
     3781 aacgcctcct cggccgcgac gtcgccgacg atcgcgagga cggcgttgtt cgggccgtag
     3841 aagctctcga agaacgcctt cacgtcggcg gaggacgccg cgtcgaggtc ggcgaaggag
     3901 ccgtagccgt cgtgcgcgtt ctcccacttc gtgaaggcgg ccccggcgac gtcggtccag
     3961 aagaagagcc cgtaggggcg gttcttgacg ttgacgcgga tctcctcctt gacgacgtcc
     4021 ttctggttct ggaggttctt ctcggagaag tcgagcatct tcatccggtc ggcctcgagc
     4081 cagaggatcg cgtcgagggc cgacaccggg gcgctcacga tgtagttcgt gtagtcgtac
     4141 cgggtggagc cgttgttgat gccgccgccc ccctcgatga cccggtcgta ggtccccttc
     4201 ggggcgttcg gcgtcccctg gaacatcatg tgctcgaaga ggtgggcgaa gccggtccg
//
LOCUS       DAOTEZ010000386         4256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_15246_length_4256_cov_5.791240, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000386 DAOTEZ010000000
VERSION     DAOTEZ010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15246_length_4256_cov_5.791240"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="P5164_16355"
     CDS             complement(<1..214)
                     /locus_tag="P5164_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRY45511.1"
                     /translation="MSWLFLSSGLFLGWSLGANDAANVFGTAVATKMVRFRTAALVAG
                     LFVVLGSVAGVSGAAGTLGRLGAIDAP"
     gene            complement(237..1328)
                     /locus_tag="P5164_16360"
     CDS             complement(237..1328)
                     /locus_tag="P5164_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013456668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRY45512.1"
                     /translation="MSWLFLTSGLFLGWSLGANDAANLFGTAVATRMLKFRTAALVGG
                     VFVVIGAWAGAHGTSKTLGRLGTIDAPAGAFVVALAAAAAVVLLLTRSGLPVSITQAI
                     VGAILGWNLFNGKPTDLKTLTQLLSSWLLSPLLSGIIAAGLFLVARRLLQRRRVHLLE
                     VDAWTRTALLVAGAFGAFSLGLNDIANVMGVFVRVSPFPAETALGPLRFTSVELLFLL
                     GGVAIAAGIATYSGRVMVRLGSGLTSLSPLAGLIVVLAAALVLFLFGSPALQRLLRAA
                     GLPGWPLVPVSATQAVVGAIVGISLVRRSGIRLKPLGQIAIGWVAAPAAALLFALVGL
                     FVVQNVFELPVSATPRPAAGASAVAGSAS"
     gene            complement(1345..1989)
                     /locus_tag="P5164_16365"
     CDS             complement(1345..1989)
                     /locus_tag="P5164_16365"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HRY45513.1"
                     /translation="MTFLFRRTRALEAQIDQFLDAVSEGCLVFEKGVDDYLRHDEPSF
                     EERRRAIGVFEGKADALRREVETRLYAESLIPDHRGDVLGLLENLDNVIDQAKQTLGQ
                     FEVERPEIPEALAPDYLALARACVQATQHLVDSCRAFFREITAVRSHIHKVYFFEKEA
                     DRLADGLKRKIFATDLELARKIHLRYFCLHVETLSDRAEGVADRLAIYTIKRTI"
     gene            complement(1986..2129)
                     /locus_tag="P5164_16370"
     CDS             complement(1986..2129)
                     /locus_tag="P5164_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45514.1"
                     /translation="MRGPDPARSPGRPAHVKWTPESGRPPLLLVVVAFLLLLAAVLAG
                     GVP"
     gene            complement(2329..2931)
                     /locus_tag="P5164_16375"
     CDS             complement(2329..2931)
                     /locus_tag="P5164_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45515.1"
                     /translation="MSNAAPVIIPRWEYRTFGESFGEAESNIRSHGEAKARSSAETYI
                     VSAESPNNTKIRDGLMDIKKLENVNGDTLEQWNPVLKSGFPLTMDVLAQVAAAWGVEL
                     PARAREACSLDEFLAEVVGAHPKLRAIAVAKERFGLTVDGCIVELANLTFDGTPIRTA
                     AVEHEDPALVIATVRKLGLDRFPNTNYIKALKRFAGLPVG"
     gene            complement(3046..>4256)
                     /locus_tag="P5164_16380"
     CDS             complement(3046..>4256)
                     /locus_tag="P5164_16380"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRY45516.1"
                     /translation="AAEEKAKSAKGTTLRSVSDKGKLEWASADGETSFRLVGRVQIDG
                     VAFSGNENRLASAVAIRRARIGYKARIGKDWVSEFDVDFAENGVDIKDAWIGYEGFKD
                     GLVQAGHFKVPFGMDTLTSSKDIWFVERAYVDAWSPDRRFGLAYATSGDRFSLKGDLF
                     AQTISVDATGIDQGWGWAARGTWAPVMKSERNAIHVGAAVNWRKPDAGSTNYGVPLAY
                     EVDFSSRPECSKASKAKFLNSPKFAQTDWVQQYAGELAGVWDAFAWQAEYQKTKVTRR
                     DGNPKLVDHDFDGWYGQVSYVLNGKRKYAADEGLVDKVSPGKGGAYEIVARYSTLDLN
                     DVTELDPAKGGSAKNFTLGANWYPNNSFRVMLNWTLVDNDEYAKPKSAYGGFTNDDFN
                     EYQLRLQFSF"
BASE COUNT          654 a         1462 c         1531 g          609 t
ORIGIN      
        1 ccggagcgtc gatcgccccg agccgcccga gcgtcccggc cgcgccggag acgcccgcca
       61 ccgagccgag gacgacgaag aggccggcga cgagagccgc cgtccggaag cggaccatct
      121 tcgtggcgac ggccgttccg aagacgttcg cggcgtcgtt cgcgccgagc gaccagccga
      181 ggaagaggcc gctcgaaagg aagagccagc tcaacgcgcg gggacctccg agggcttcag
      241 gaggccgagc cggccacggc gctcgccccg gcggccggcc gcggcgtggc gctcacgggg
      301 agctcgaaga cgttctggac gacgaagagg cccacgagcg cgaacaggag ggccgccgcg
      361 ggcgcggcga cccagccgat cgcgatctgc ccgagaggct tgagccggat gccgctcctc
      421 ctcacgaggg agatcccgac gatcgcgccg acgacggcct gggtcgccga aaccgggacg
      481 agcggccagc cgggtagccc cgccgcccgg aggaggcgct ggagcgcggg agagccgaag
      541 aggaagagga cgagggccgc cgcgaggacg acgatcagcc cggcgagcgg agagagggac
      601 gtcagccccg atccgagccg gaccatgacc cggcccgagt aggtcgcgat ccccgcggcg
      661 atcgcgactc ctccgagcag gaagagcagc tcgacgctcg tgaatcggag cggcccgagc
      721 gccgtctccg caggaaaggg cgagacgcgg acgaagaccc ccatcacgtt cgcgatgtcg
      781 ttcagcccga ggctgaaagc gccgaaggct ccggcgacga ggagagccgt gcgggtccag
      841 gcgtccacct cgaggaggtg gacccgtcgc cgctgaagga gccgacgggc gacgaggaag
      901 aggcccgcgg cgatgatccc cgacaggaga gggctgagga gccacgagga gaggagctgc
      961 gtcagcgtct tcaggtccgt cggcttcccg ttgaagaggt tccagccgag gatcgcgccg
     1021 acgatcgcct gggtgatcga gacggggagc cccgaccgcg tcaggaggag gacgaccgcg
     1081 gccgccgccg cgagggcgac cacgaaggcg cccgccgggg cgtcgatggt accgagccgg
     1141 ccgagcgtct tcgaggtccc gtgagcgccg gcccacgccc cgatcacgac gaagactcca
     1201 ccgaccaggg ccgccgttcg gaacttcagc atccgggtcg cgacggcggt cccgaagagg
     1261 ttcgccgcgt cgttggcgcc gagcgaccag ccgaggaaga ggccgctcgt caggaagagc
     1321 caggacatcc cgtccgcctc ccgctcagat cgtccgcttg atcgtgtaga tcgcgaggcg
     1381 gtcggcgacc ccttccgccc ggtccgacag cgtctcgacg tggaggcaga agtagcggag
     1441 gtggatcttc cgcgcgagct cgaggtccgt cgcgaagatc ttccgcttca ggccgtccgc
     1501 gaggcgatcc gcctccttct cgaagaagta gaccttgtgg atgtggctcc tcaccgccgt
     1561 gatctcgcgg aagaaggccc ggcaggaatc gacgaggtgc tgcgtcgcct ggacgcaggc
     1621 ccgggccagg gcgaggtagt ccggggcgag cgcctccggg atctcgggcc gctccacctc
     1681 gaactgcccg agggtctgct tggcctggtc gatcacgttg tcgaggttct cgaggaggcc
     1741 gaggacgtcg ccccggtggt ccgggatgag cgactcggcg tagagccgcg tctcgacctc
     1801 gcgccgcagc gcgtccgcct tcccctcgaa gacaccgatc gccctccggc gctcctcgaa
     1861 gctcggctcg tcgtggcgga ggtagtcgtc gacccccttc tcgaagacga ggcacccctc
     1921 gctcacggcg tcgaggaact ggtcgatctg cgcttccagc gcgcgcgtcc gtcgaaagag
     1981 gaacgtcacg ggacacctcc ggccagcacg gcggcgagga gcagcaggaa ggcgacgacg
     2041 acgaggagga gcggcgggcg cccggactcc ggcgtccact tcacgtgggc cggcctgccg
     2101 ggagagcgcg ccgggtcggg gccccgcacg tcagacccgg cgcccgcgcg cagacaccgc
     2161 cgcccggggc ccttcgccgc ctcgatcggg cgtccagcgc tctctctgtc tgagcatctc
     2221 ggcctccgat gccccccgtc ggaagaagag gcgggcgcgg acgacccgcg cccgccggaa
     2281 ggaggaggag cggaggggcg acgacccccc tccgccggaa cgcgccgatc agccgaccgg
     2341 cagaccggcg aaccgcttga gcgccttgat gtagttcgtg ttcgggaacc tgtcgaggcc
     2401 gagcttcctc accgtcgcga tgacgagcgc ggggtcctcg tgctccacgg cggccgtccg
     2461 gatcggcgtg ccgtcgaacg tcaggttcgc gagctcgacg atgcagccgt cgaccgtgag
     2521 gccgaaccgc tccttggcga ccgcgatcgc gcggagcttc gggtgggcgc ccacgacctc
     2581 cgcgaggaac tcgtcaagcg agcacgcctc gcgggcgcgg gcggggagct cgacgcccca
     2641 ggccgcggcc acctgggcga ggacgtccat cgtgagaggg aaacccgact tcaggaccgg
     2701 attccactgc tcgagcgtgt cgccgttgac gttctcgagc ttcttgatgt ccatcaggcc
     2761 atcgcggatc ttggtgttgt tcgggctctc ggccgagacg atgtacgtct cggcgctcga
     2821 gcgcgccttc gcttcgccgt gggaccgtat gttcgactcg gcctcgccga agctctcgcc
     2881 gaaggtcctg tactcccagc gggggatgat gacgggggcc gcgttgctca tgtcgatgct
     2941 cctggcacgg gcggcgggag ggcccgaccg cgggttcgtc gatggggcga aggccgcgag
     3001 acgccctccc ggaggaggtc ctcccgcggc cggtccgccg ggtgactaga agctgaactg
     3061 gagccggagc tggtactcgt tgaagtcgtc gttcgtgaac ccgccgtagg ccgacttcgg
     3121 cttggcgtat tcgtcgttgt cgacgagcgt ccagttcagc atcacgcgga agctgttgtt
     3181 cgggtaccag ttcgctccga gggtgaagtt cttcgcgctg ccccccttcg ccgggtcgag
     3241 ctccgtgacg tcgttcaggt cgagcgtgga gtagcgggcg acgatctcgt acgccccgcc
     3301 cttgcccggg gagaccttgt cgacgagccc ctcgtcggcc gcgtacttcc gcttgccgtt
     3361 gaggacgtag gagacctgcc cgtaccagcc gtcgaagtcg tggtcgacga gcttcgggtt
     3421 gccgtcgcgc cgcgtcacct tcgtcttctg gtactccgcc tgccaggcga aggcatccca
     3481 gacgcccgcg agctcgccgg cgtactgctg cacccagtcg gtctgcgcga acttggggct
     3541 gttgaggaac ttcgccttgc tcgccttcga gcactcgggg cgcgaggaga agtcgacctc
     3601 gtaggcgagc gggaccccgt agttcgtcga gccggcgtcc ggcttcctcc agttcacggc
     3661 cgcgccgacg tggatcgcgt tccgctcgga cttcatcacg ggcgcccagg tgccgcgcgc
     3721 cgcccagccc cagccctggt cgatgccggt cgcgtcgacg gagatcgtct gggcgaagag
     3781 gtcgcccttc agggagaagc ggtcgcccga ggtggcgtac gcgaggccga agcggcggtc
     3841 gggagaccag gcgtcgacgt acgcgcgctc gacgaaccag atgtccttgg aggacgtcag
     3901 cgtgtccatg ccgaacggca ccttgaagtg gccggcctgg acgaggccgt ccttgaaccc
     3961 ttcgtagccg atccaggcgt ccttgatgtc gacgccgttc tcggcgaagt cgacgtcgaa
     4021 ctcggagacc cagtccttcc cgatccgggc cttgtagccg attcgggccc ggcggatcgc
     4081 cacggcgctc gcgaggcggt tctcgttgcc gctgaacgcg accccgtcga tctgtacgcg
     4141 gccgacgagg cggaaggagg tctcgccgtc ggcgctcgcc cactcgagct tgcccttgtc
     4201 gctcacggag cggagcgtcg tccccttcgc ggacttcgcc ttctcctcgg cggccg
//
LOCUS       DAOTEZ010000387         4175 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_15671_length_4175_cov_4.143932, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000387 DAOTEZ010000000
VERSION     DAOTEZ010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15671_length_4175_cov_4.143932"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="P5164_16385"
     CDS             <1..181
                     /locus_tag="P5164_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006635013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HRY45517.1"
                     /translation="DLRIHGPELWRRFNAGRDETLWYLESLVAAFREAGSTPIVEELA
                     RTVAELRFVSGNGHG"
     gene            298..2784
                     /gene="ptsP"
                     /locus_tag="P5164_16390"
     CDS             298..2784
                     /gene="ptsP"
                     /locus_tag="P5164_16390"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HRY45518.1"
                     /translation="MLKAPLSGVVVPIEKVPDPVFAHRLLGDGVAIDPVSAILLSPCD
                     GVVATVHPSGHAVAIAADAGVEVLLHVGLDTVALGGRGFVPRVAQGRRVAAGEPLLAF
                     DADLVARSARSLVTPVVVTSRERVTSARFATGEVTAGVDDLMVLTLVPSAFPLVAPVA
                     GERTRDVRVMRRLGLHARPAAALASAARRFRAEVLVETGTSMASARSIVGLLSLGVVH
                     GEVVRLRATGPDAGKALAALARVLEAEEGHEAAAPLPSAPRPARSDARVFPGIPSAPG
                     CATGPVVRLAREEPEVVEEGSDGRTERRDLDFALEEAKVQLRALEARLGAEAGSEHAD
                     IFEAHVALLEDPLLLSEAEHEIAAGKSAAFAFREAVRRYSDRIASLPNPALAARAADL
                     RDAGGRVLRILAGSRSSAPAPPEGAIVVSEDLTPSEAATLGRGGVAGFCTVAGGATSH
                     VALLARSMGIPAVAGIDARALEVEPGVPGVLDGDAGELRLAPDDAERESVRERCARRA
                     ARRTADRLAALEPAWTRDGVRVTVLGNAGSLADAEDAIAAGAEGIGLLRSELLFLERA
                     NVPTEDEQEAVYRAVAEAVGPGRPLVVRTFDVGGDKPLRWLPLPEEENPALGERGLRV
                     FIDRPDLFRAQVRALLRVPLEYDVRVTFPMVATLDEWRGALADVEEERAALGAPPRVA
                     GMTVEVASAAILADAFLREAGFATIGTNDLTQFTLAMDRGHPRLSPRADALDPAVLHL
                     VARTAAAGRKRGVPVAVCGALAGDLAAVPLLLGLGVGELSVGAAQVPSVKARVRELSL
                     EECRSLAVGALEAGTAAEVRALLAAARGAA"
     gene            2781..>4175
                     /locus_tag="P5164_16395"
     CDS             2781..>4175
                     /locus_tag="P5164_16395"
                     /inference="COORDINATES: protein motif:HMM:NF014437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRY45519.1"
                     /translation="MTRGGALARLQTVGRALMLPVSVLPVAGLLLGLGNARLGLLPAG
                     AAHLLAEAGGAVFAALPLVFAIAVALGLSGNDGAAAVAAVVGYVVFVATLGAGANALG
                     LPTVRVLGFESEETGVFGGILVGLLAAALVRRFSRSDLPAALGFFGGPRLVPILTGLA
                     ATLLGLALSVVWPPASDAVRLLSDRVLEAGPAAAVFAYGVVDRLLLPFGLHHIWNVPF
                     FFEIGSFVDATGKTVHGDITRFFAGDPAAGILSGGFLFKMWGLPAAALAIWRCARPEN
                     RARVGGIMVSAAATSFLTGITEPIEFSFLFLAPFLYALHALLAGLAFVVANLAGMKLG
                     FTFSHGFFDLALYWPMDTRPWLVLLLGPIWGLVYYGVFRAAILRFDLKTPGREEPAAA
                     PEPGPVAGRPAPVDAGGLAERLVAAFGGARNIESLDACITRLRVTVRNLDAADEKALR
                     ALGAAGVVVIGNAVQ"
BASE COUNT          416 a         1533 c         1607 g          619 t
ORIGIN      
        1 ggacctccgg atccacgggc cggagctctg gcggcgcttc aacgccgggc gcgacgagac
       61 gctctggtac ctcgagtctc tcgtcgccgc gttccgcgag gcggggtcga cgccgatcgt
      121 cgaggagctc gcgcggaccg tcgccgagct ccgcttcgtt tccggcaacg ggcacggctg
      181 agggccgcgc ccgaccgtcg ccgcccggcc tccgcaccct atactggccg cgtcccccgt
      241 gccatgaacg agccgtcgac aggcggccct cgggccgcgt ctcccgagcc gacgctggtg
      301 ctgaaggctc cgctttcggg cgtcgtcgtg ccgatcgaga aagtccccga cccggtcttc
      361 gcgcaccggc tcctcgggga cggcgtggcg atcgaccccg tctcggcgat cctcctgtcg
      421 ccgtgcgacg gcgtcgtcgc gacggtgcac ccgtccgggc acgcggtcgc gatcgcggca
      481 gacgcggggg tcgaggtgct gctccacgtc gggctggaca ccgtggcgct cggcgggcgc
      541 gggttcgtgc cgcgcgtcgc gcaggggcgg cgcgtcgccg ccggcgagcc gctcctggcg
      601 ttcgacgccg acctcgttgc ccgttcggcc cggagcctcg tgacgccggt cgtcgtgacg
      661 agccgcgagc gggtgacgtc ggcacgcttc gccacgggag aggtgacggc gggggtggac
      721 gacctcatgg tactgacgct cgtcccgtcg gcgttccccc tcgtggcgcc cgtggccggc
      781 gagcggacgc gcgacgtccg cgtgatgcgc cggctcggcc tccacgcgcg gcccgccgcg
      841 gcgctcgcct cggcggcgcg ccgtttccgg gcggaggtgc tcgtcgagac ggggacgtcg
      901 atggcgagcg cgcgaagcat cgtcgggctc ctctctctcg gcgtcgtcca cggcgaggtg
      961 gtgcgccttc gcgcgacggg gccggacgca ggcaaggcgc tcgcggccct cgcccgggtc
     1021 ctggaggccg aggaggggca cgaggccgcg gcgccgctcc cctcggcgcc gcgcccggcc
     1081 cgttcggacg cccgggtctt cccggggatc ccgtcggcgc ccggctgcgc gaccgggccg
     1141 gtcgtccgcc tcgcgcgcga ggagccggag gtcgtcgagg aggggagcga cgggcggacc
     1201 gagcgccgcg acctcgactt cgcgctcgaa gaggcgaagg tgcagctccg tgccctcgag
     1261 gcgcggctcg gcgccgaggc ggggagcgag cacgccgaca tcttcgaggc gcacgtggcg
     1321 ctgctggagg acccgctcct cctctcggaa gccgagcacg agatcgccgc cggcaagagc
     1381 gcggcgttcg cgttccggga ggcggtccgg cgctactcgg acaggatcgc gagccttccg
     1441 aacccggcgc tggcggcgcg ggccgccgac ctgcgcgacg cgggaggccg cgtcctccgg
     1501 atcctcgcgg gatcgcgctc gtccgccccg gcccctccgg agggtgcgat cgtcgtgagc
     1561 gaggacctga cgccgtccga ggcggccacg ctcggccggg gtggcgtcgc cggtttctgc
     1621 accgtcgccg gaggggccac gtcgcacgtg gcgctgctcg cgcggtcgat ggggatcccg
     1681 gcggtcgccg ggatcgacgc ccgcgccctc gaggtggagc cgggcgtgcc gggcgtcctc
     1741 gacggggacg ccggagagct gcgcctcgct cccgacgacg ccgagcgcga gtccgttcgg
     1801 gagcgctgcg cgcggcgcgc ggcgcgacgg acggccgacc gcctggccgc gctcgagcct
     1861 gcctggacgc gggacggcgt gagggtgacg gtcctgggca acgcggggag cctcgccgac
     1921 gcggaggacg cgatcgcggc gggcgcggag gggatcgggc tgctccgctc cgagctgctg
     1981 ttcctcgaga gggcgaacgt cccgacggag gacgagcagg aggcggtcta ccgggccgtc
     2041 gcagaggcgg tggggccggg gcggccgctc gtcgtccgga cgttcgacgt ggggggcgac
     2101 aagccgctcc gctggcttcc gctgccggag gaggaaaacc cggccctcgg ggagcgcggg
     2161 ctgcgcgtct tcatcgaccg tcccgacctc ttccgcgccc aggtgcgggc tctcctcagg
     2221 gtgcctctgg agtacgacgt gagggtgacg ttcccgatgg tcgcgacgct cgacgagtgg
     2281 cgcggggcgc tcgcggacgt cgaggaggag cgcgcggcgc tgggggcgcc gccgagggtg
     2341 gccgggatga ccgtggaggt ggcctcggcc gcgatcctcg cggacgcgtt cctgcgcgag
     2401 gcgggcttcg ccacgatcgg aacgaacgac ctgacgcagt tcacgctcgc gatggaccgc
     2461 gggcacccgc gcctctcgcc gcgggccgac gccctcgacc cggctgtcct ccatctcgtg
     2521 gcgcgcaccg ccgccgcggg gcggaagcga ggagttccgg tggcggtctg cggcgcgctg
     2581 gcgggagacc tcgcggcggt gccgctcctc ctcggcctcg gggtcggaga gctctcggtc
     2641 ggggcggccc aggtcccgtc cgtgaaggcg cgggtccgcg agctctcgct cgaggagtgc
     2701 cggtctctgg ccgtgggggc gctggaggcg gggacggcgg ccgaggtgcg cgctctcctc
     2761 gcggccgcgc ggggcgcggc gtgacgaggg gcggcgccct cgcgcggctc cagacggttg
     2821 gccgggcgct catgctcccg gtctccgtcc tccccgtcgc cgggctgctt ctcggcctgg
     2881 ggaacgcccg gctcggactc ctcccggccg gcgcggcgca cctcctcgcg gaggccgggg
     2941 gcgccgtctt cgcggctctc ccgctcgtct tcgcgatcgc ggtggcgctc ggcctctccg
     3001 ggaacgacgg cgccgcggcg gttgctgcgg tcgttggcta cgtcgtcttc gtcgccactc
     3061 tcggcgcagg cgcgaacgcg ctcggcctgc cgacggtgcg ggtcctcggc ttcgagtcgg
     3121 aggagacggg cgtcttcggc gggatcctcg tcggactcct cgccgccgcg ctcgtccgga
     3181 ggttctcgcg gtcggacctt ccggcggcgc tcggattctt cggcgggccc cggctcgtcc
     3241 cgatcctgac gggcctcgcg gcgacgctcc tcggcctggc gctctccgtc gtctggcctc
     3301 cggcgagcga cgcggtccgc ctcctctccg accgcgtcct cgaggcgggg cccgcggccg
     3361 cggtcttcgc ctacggcgtc gtcgaccggc tcctcttgcc gttcggcctc caccacatct
     3421 ggaacgtccc gttcttcttc gagatcggct cgttcgtcga cgccacgggg aagaccgttc
     3481 acggcgacat cacgcgcttc ttcgcggggg acccggcggc gggaatcctc tcgggcggct
     3541 tcctcttcaa gatgtggggt ctccctgcgg ccgctctcgc catctggcgc tgcgcccgtc
     3601 ccgagaaccg cgcgcgcgtt ggcgggatca tggtctcggc cgcggcgacg tcgttcctga
     3661 cggggatcac cgagccgatc gagttctcgt tcctcttcct cgcgccattc ctctacgcgc
     3721 tccacgcgct cctcgcgggc ctggccttcg tcgtggcgaa cctcgccggg atgaagctcg
     3781 ggttcacgtt ctcgcacggc tttttcgacc tcgccctcta ctggccgatg gacacgcggc
     3841 cctggctggt gcttctcctc gggccgatct ggggtctcgt ctactacggc gtcttccggg
     3901 cggcgatcct gcgtttcgac ctgaagacgc cagggaggga ggagccggcg gctgcgcccg
     3961 aacccggccc ggtagccggg cgtccggcgc ctgtcgacgc gggtggcctc gccgaacgtc
     4021 tcgtcgccgc cttcggaggg gcacggaaca tcgagagcct cgatgcgtgc atcacgcgac
     4081 tccgggtcac cgtccgaaac ctggacgccg ctgacgagaa ggcgctccgg gcgctcgggg
     4141 cggcgggcgt cgtcgtcatc ggcaacgcgg tccag
//
LOCUS       DAOTEZ010000388         4174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_15676_length_4174_cov_6.074047, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000388 DAOTEZ010000000
VERSION     DAOTEZ010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15676_length_4174_cov_6.074047"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..552
                     /locus_tag="P5164_16400"
     CDS             112..552
                     /locus_tag="P5164_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45520.1"
                     /translation="MTAAQLLGLISAALLIQLAVGVGVVLRRRRIDGRTVPAEAPATM
                     PLLVPTQAPPRLDGPLAALAAGDFETTVERVRAERRRTPAARELDLVEAAALGARYVL
                     EGRRDGSLLDRARASLASFRSKGGSARAEAALLSPALRALLETR"
     gene            complement(560..1744)
                     /locus_tag="P5164_16405"
     CDS             complement(560..1744)
                     /locus_tag="P5164_16405"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRY45521.1"
                     /translation="MPEGSHRYVLVVDGREIELAAGEVTLGRSRSSTVRVDHESVSRS
                     HALLTLSDGKAVLKDLNSSNGTFVAGRRILNETILRSGDRLQLGAAVLEYRLLEPTGS
                     LEKTAHLTTTAAEALSAVAAAHGDPAARPDAGPEPSASAALEISAHDLFRETDENARK
                     AGLAPVGAAAALAAVHGSGVRAEPPVAPPPPAEAPKGGPAPAVPEATLSQFPIRSDGR
                     PSAVRPAPAVKVRRPRSSTSLATVAETAGLFPRLLATLVDGVILLALDLLLLSPVFLV
                     LLFREAFQSADLTRDWAFLAIAGGCAVLVLGANLWYVVGGWARTGRTPGKALLRLSVV
                     PVASGRTAPGIGLGPAFGRFLAWNLSGAILGIGHLVALFRKDRRTLHDLMAGTRVVRG
                     RS"
     gene            complement(3538..3834)
                     /locus_tag="P5164_16410"
                     /pseudo
     CDS             complement(3538..3834)
                     /locus_tag="P5164_16410"
                     /inference="COORDINATES: protein motif:HMM:NF036483.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          558 a         1538 c         1608 g          470 t
ORIGIN      
        1 ggcagcagac ttgatgccac tcaagcgggc tacgcgggcg acctcccaga attcccttgg
       61 ctcccgacgc gctcaacgac gcgacgcctc cgggcgcatg gaatcccgta cgtgaccgct
      121 gcacaacttc tcggactcat ctcggctgcc ctgctgatac agctggccgt gggcgtcggt
      181 gtggtcctgc gacgtcggag gatcgacggg cggacggtgc cggccgaggc tcccgcgacg
      241 atgccgctcc tcgtcccgac gcaggcccct ccccggctcg acgggccgct cgcggccctc
      301 gccgccggcg acttcgagac gacggtcgag agggtccgcg cggagcgccg gcggactccc
      361 gccgcccggg agctggacct cgtcgaggcg gccgccctcg gcgcgcgcta cgtcctggaa
      421 ggccggcggg acggctcgct cctcgaccgc gcccgggcga gcctcgcctc gttccgcagc
      481 aagggaggct cggcccgcgc ggaggcggct ctcctctctc cggcgctccg ggctctcctc
      541 gagacgcgct gaggccgggc tacgaccggc cgcggacgac gcgcgtcccg gccatcaggt
      601 cgtgcagcgt ccggcggtcc tttcggaaaa gcgccacgag atggccgatg ccgaggatcg
      661 ctccggagag gttccaggcg aggaaccgcc cgaacgcggg gccgaggccg atccccgggg
      721 ccgtgcggcc ggatgcgacg gggacgaccg agagacgcag gagcgccttg cccggcgtcc
      781 gccccgtccg cgcccagccg cccacgacgt accagaggtt cgccccgagg acgaggaccg
      841 cgcagccgcc cgcaatggca aggaaggccc agtcccgcgt caggtcggcc gactggaacg
      901 cctcgcgaaa aaggaggacg agaaagacgg gcgagaggag caggaggtcc agcgccagga
      961 ggatgacgcc gtcgacgagg gtcgcgagga gccgcgggaa gaggccggcg gtctcggcca
     1021 ccgtcgcgag cgacgtcgag gagcgcggcc ggcggacctt cacggcggga gcgggacgga
     1081 ccgcgctcgg acggccgtcg ctccgtatcg ggaactggga gagcgtcgcc tccggaacgg
     1141 cgggcgccgg tccacccttc ggcgcctccg ccggcggcgg cggggcgacc ggcggctcgg
     1201 cccggacccc cgagccgtgg acggccgcga gcgcggccgc ggccccgacc ggcgcgaggc
     1261 ccgcctttcg ggcgttctcg tccgtctccc ggaagaggtc gtgggccgag atctcgagcg
     1321 cggcgctcgc cgacggctcc gggccggcgt cggggcgtgc cgcagggtcg ccgtgcgccg
     1381 ccgcaacggc cgagagcgcc tcggcggccg tcgtcgtcag gtgcgccgtc ttctcgagcg
     1441 acccggtcgg ctccaggagc cggtactcca ggacggcggc gccgagctgg agccggtcgc
     1501 cgctccggag gatcgtctcg ttcaggatcc tccggccggc gacgaacgtc ccgttcgagg
     1561 agttcaggtc cttgaggacc gccttcccgt ccgagagcgt gaggagcgcg tggctcctcg
     1621 agacggactc gtggtcgacg cggacggtcg acgagcggct ccggccgagc gtgacctctc
     1681 cggccgcgag ctcgatctcc cggccgtcga cgacgaggac gtagcggtga ctcccttcgg
     1741 gcacggcggc gccgattgta ggcgagcggg agcgcccgag gcggcccgga gcgcctcggc
     1801 cgtcgccgtg gtcccgcccg agagcagccc cgcgacgttc ccggcgtaga cgacccgtcc
     1861 cccttcggcc ccgccgccgg ggcccagctc gaggagccag tcgcaggcga ggaggaaggc
     1921 ggggttgtgc tccaccgcga ggacggagtg tcccgccgcg aggagccggc ggaagacggc
     1981 gaggaggacg tcgacgtcgc cggggtggag ccccgtcgtc ggctcgtcga agaggaagag
     2041 cgccggcttc ccggggcccg gcttcaggaa cgaggcgacc ttcagccgct gcgcctcccc
     2101 tcccgagagc gtcgcggtcg cctgcccgag ccggaggtag ccgagaccgg cctcggagag
     2161 cggcgcgagg cgcgaggcga tccccggcag gtccgcgaag agctcgatcg cctcgtcgac
     2221 cgtcgtcccg agaagctcgt ggacgttccg gccgcgaacc ctcacgccca ggacctcggg
     2281 ggtgaagcgt cgcccgtcgc aggcgtcgca gacgaccgtg acgtcggcca ggaactgcat
     2341 gtcgaccgtg acgacaccgg agccctcgca ccgctcgcag cggccgccgg gcgcgttgaa
     2401 gctgaagctc cccttcgtca gccccgcggc gcgggccgcc gggctccggg cccagagggc
     2461 gcggacctcg tcccacgcct tcgtgtacgt cgcgggattc gagcgggccg agcggccgag
     2521 cggcgactgg tcgacgagga cgacgtcgtg gaaggccgcg agcccctcga tccgatcgtg
     2581 cgctccccgc tcgagggcgt ccgcctcctt ccggccgagg gcgcggagcg ccccggaggc
     2641 gaggacgtcg acgacgaggg acgacttgcc ggagccggac ggcccgcaga tccccgcgag
     2701 gaccccggtc gggacctcga cggtgacttc ggcgaggttg ttcgcccgcg cgccgacgat
     2761 gcgaatcgcg tcgcgcgggt aggccgccgg gcgctcggcc acggcgctcg aggccgccgt
     2821 ccggagcgcc gcacccgtcg ccgtcgcggc gcgggcgagg gcccgcggcg ttccctcgaa
     2881 gacgaggctc ccgccgtgcg ccccggcgcg cggcccgagg tcgacgacgt ggtccgcggc
     2941 gcggatgacg tcgaggtcgt gctccacgac gcagacggcg ttccccgcgt ccgcgaggcg
     3001 gcggaggacg gcgaggaggc gggccgtgtc ggccgggtgg aggcccaccg tcggctcgtc
     3061 gaggacgtag agcgttccgg tcagagagtt cccgagcgcg gcggcgagct ggacccgctg
     3121 cgcctcgccc cccgagaggg tgcgcgtcgt gcgcgacagc gtcaggtacg agagcccgac
     3181 gtcgacgagc gtcgcgacgc gcctgccgag gtcatcgacg agcgccccgc cccgggccct
     3241 ctcgaccggg tcgagcggga gcgagcgcag ccgctcgagc agtgcgccga gagggaggtc
     3301 gcagagctcg gggagcgtcg cgccgccgac gcggaccgcg cgcgcctcgg gcgtcagccg
     3361 cgccccgagg cactccgggc agcgctcgta gccgcggtag cgggcgatca tgacgcggac
     3421 ctggaccttg tagcgcttcg tctcgagcca gtcgaagagc cctttcaccc cgtaccagtc
     3481 tccgtcgccg tcccagacga gctcccgctc ccgcggcgtc atctcccgcc acggcacgtt
     3541 cttccggatc ccgagccgct tgcaggcgcg ccagaggtcg ggataggcgc tcgcgtaggc
     3601 gggcgtgttg aacggcgcga aggggcgctc gaggagcgtc ctcgacgggt cgggcaggat
     3661 cttctccgcg tcgaggccgg cgatccgccc gaagccctcg caggcgcgac aggcgccgcg
     3721 cggcgagttg aacgagaagt gggcgggcgt cggctcctcg aacgtccggc cgcagccgtt
     3781 gcaggcgagg ccgcggagga agcgaagctc gcgccccgtc gccgcgtcgc gcacgagaag
     3841 cgtcccctcg ccgaccgcga aggccccgtc gagcgaggcg gcgagctcgg cgtccccggt
     3901 gcggaggacg tggcggccga cgacgaggcg gacgagaccg tccgctccgg cgggcggggg
     3961 cgcctccccg tcgaacgaga cgacgctccc gtcggagtcg accgcccgga agaggccgga
     4021 gcggacccag agcggcgcgc gctcgcggcg ttccgcctcg ggtcgcgggg ccagcacgag
     4081 gaggcgcacc ccctcgccga gctcggaccg gagcgcctcc gcgacgctcg ccggcgagtc
     4141 gcgcttcacc gcgcggccgc aggcggggca gaca
//
LOCUS       DAOTEZ010000389         4167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_15714_length_4167_cov_7.863570, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000389 DAOTEZ010000000
VERSION     DAOTEZ010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15714_length_4167_cov_7.863570"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="P5164_16415"
     CDS             complement(<1..242)
                     /locus_tag="P5164_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     N-terminal domain-containing protein"
                     /protein_id="HRY45522.1"
                     /translation="MATKVGINGFGRIGRQVLKAIRDKYPTELEVVAVNDIGDLKTMA
                     HLLKYDSTYGRFDGTVEVAGEDLLVDGRHLKMLKET"
     gene            complement(328..885)
                     /locus_tag="P5164_16420"
     CDS             complement(328..885)
                     /locus_tag="P5164_16420"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRY45523.1"
                     /translation="MARPASRSASAPSPRATLTLEEDGARHTFHLDHAATVGRDPACD
                     ILLSSPTVSRRHALVFPDGEGWTLRDLGSGNGTFLDARRVDEAPLPNGVALRFGSVPA
                     WFEEEPAEPLTPSQKLQRALTQTLSIRPAKRARKKAAVAALAGGVALLLGTTFWNRGC
                     SARGAAPEQTTEASAPAAGSTERPG"
     gene            complement(889..2283)
                     /locus_tag="P5164_16425"
     CDS             complement(889..2283)
                     /locus_tag="P5164_16425"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HRY45524.1"
                     /translation="MDPRSFLRALYRAAVAAVEPGRLVSEALSRRGSAVVVRSVEDPG
                     RREFVFVPRRVVLLAVGKAAPAMAAAALRAMGDLVDEAVVVAPAGVLPEELPPGGRFL
                     PSAHPVPDERSLAAGREALALAAGLGRDDLLVVLLSGGGSSLLALPPEEVPLADKVRT
                     VSLLAAAGAPATDVNLVRGALSRLKGGRLAAAARQADVVTLVLSDLGDTGWHLVASGP
                     TFGVPPSRRTAAALLETWRIQPLVPSSVRSFLASPSANEEVRDGGQRWGVLLGDVRTA
                     LEGARREALRFGADVRVLPELLSGEARAAARRLAVAGASAGNLVRRAGAPPRRLVTLF
                     GGETTVTVKGRGQGGRCRELALATAFHVDGIAGANLLVAGTDGVDHTPDAAGAFVDHA
                     TLARAAALGLDPAAALAGNDSGPFFAALGDAFAPGPTGTAVGDVAFVLAPGGEDVPAA
                     LDADEEGIPLPDRI"
     gene            2365..2655
                     /locus_tag="P5164_16430"
     CDS             2365..2655
                     /locus_tag="P5164_16430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45525.1"
                     /translation="MPQVDVDRIVGSIRDGAGAVREKTQDAVEDVADKIEDALEKAGT
                     EGRKIQREVKEELARRWRQVDRVGRENAFLMAAGALAVGVLVGWLISRDRRG"
     gene            2663..3913
                     /locus_tag="P5164_16435"
     CDS             2663..3913
                     /locus_tag="P5164_16435"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY45526.1"
                     /translation="MDLVARFSELKSGFARSFWVANVLELFERFSFYGSKIVLAVFLA
                     ETVGLGPFGVSLVGLYGFAVYFLPILAGPVVDRFGFKRSLAACFAIFSFGYFLIGLSG
                     MPMGQPLVQAVGTKTWVVCALLITAVGGSLIKPCIVGTVARTTTHETKSLGYSIYYTL
                     VNIGGWLGPVLASQVRVSLGIAQVLVGASLVSFLLFLATLAFFKEPERDPAAEGRTLG
                     KVLRDAGLVLANGRFIGFLVIFTGFWVMFWQVYDLFPFYVRDVLKVERFEFIASLESF
                     SVIFLTVPVAALMKKVRAVPQMTLGLAVGSCSWLLLVVSQTWQAAAAAMFLLALGEVL
                     QAPRYYEYIADLAPKEQVGTFMGFAFLPIALGALLAGPLGGFLLQHYLKDTMQPAKAW
                     TILSCLGFVSTAALLAYDRWVARR"
     gene            complement(3986..>4167)
                     /locus_tag="P5164_16440"
     CDS             complement(3986..>4167)
                     /locus_tag="P5164_16440"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY45527.1"
                     /translation="DGLMPGQTLADARDAFERRLVVRVLEECRGNVSRAAERLGLDRT
                     TLHRRLRAWGLTDDK"
BASE COUNT          531 a         1494 c         1546 g          596 t
ORIGIN      
        1 tcggtctcct tcagcatctt caggtgccgg ccgtcgacga ggaggtcctc gccggcgacc
       61 tcgaccgtcc cgtcgaagcg tccgtaggtc gagtcgtact tgaggaggtg ggccatggtc
      121 ttgaggtcgc cgatgtcgtt gacggcgacg acctcgagct cggtggggta cttgtcgcgg
      181 atcgccttga ggacctgccg gccgatgcgg ccgaacccgt tgattccgac tttcgtggcc
      241 atcttgggcg tctccttccg ggggctttcc ccgcgggtga tgctccgtta tacgacggcc
      301 ggccggctgc gggagacccg aaagccctca gcccggccgt tccgtggagc cggcggccgg
      361 ggcggaagcc tcggtcgtct gctccggggc ggcgccccgg gccgagcagc cgcggttcca
      421 gaacgtcgtg ccgaggagga gcgccacccc gcccgccagg gccgcgaccg ccgccttctt
      481 ccgcgcccgc ttcgccggcc ggatcgagag ggtctgcgtg agcgccctct ggagcttctg
      541 gctcggcgtg agcggctccg ccggctcctc ctcgaaccag gccgggacgc tgccgaaccg
      601 gagcgcgacg ccgttcggga ggggcgcctc gtcgacgcgg cgggcgtcga ggaacgtccc
      661 gttgccgctc ccgaggtccc gcaaggtcca cccttcgccg tcggggaaga cgagcgcgtg
      721 gcggcgggag accgtcggcg aggagaggag gatgtcgcac gccgggtcgc gccccacggt
      781 cgctgcgtgg tcgaggtgga acgtgtgccg ggcgccgtct tcctcgagcg tcagggtggc
      841 ccggggagaa ggcgcggagg cggagcggga ggcggggcgg gccatcgttc agatccggtc
      901 ggggaggggg atcccctcct cgtccgcgtc gagggccgcg gggacgtcct cccctcccgg
      961 ggcgaggacg aaggcgacgt cgccgacggc ggtcccggtc ggcccggggg cgaaggcgtc
     1021 cccgagcgcg gcgaagaacg ggcccgagtc gttcccggcg agcgccgcgg ccgggtcgag
     1081 gccgagcgcc gcggcgcggg cgagggtcgc gtggtcgacg aaggcgccgg cggcgtcggg
     1141 ggtgtggtcg accccgtccg tgccggccac gaggaggttc gcgccggcga tcccgtcgac
     1201 gtggaacgcg gtcgccaggg cgagctcgcg gcagcgcccg ccctgccccc gccccttcac
     1261 ggtcacggtc gtctcccctc cgaagagggt gacgagcctc ctcggcggcg cgccggcgcg
     1321 gcggacgagg ttcccggcgg aagcgccggc gacggcgagc cggcgggcgg cagcgcgcgc
     1381 ctcgcccgag aggagctcgg gaaggacgcg aacgtcggcc ccgaagcgga gcgcctcgcg
     1441 ccgcgccccc tcgagggccg tccggacgtc gccgaggagg acgccccagc gctggcctcc
     1501 gtcgcggacc tcctcgttcg cggacggcga ggcgaggaag gagcggacgg aggagggaac
     1561 gagcggctgg atgcgccacg tctcgagcag ggcggcggcg gtgcgccgcg agggcgggac
     1621 cccgaaggtc ggaccggagg cgacgaggtg ccaccctgtg tcgccgaggt cggagaggac
     1681 gagcgtcacg acgtcggcct gccgggccgc ggcggcgagc cgccccccct tcaggcgcga
     1741 gagcgcgccg cgcacgaggt tcacgtccgt ggccggcgct cccgcggcgg cgaggaggga
     1801 gacggtccgc accttgtcgg cgagcggcac ctcctccggg ggcagggcga gaaggctcga
     1861 gcccccgccc gacaggagga cgaccaggag gtcgtcccgc ccgaggccgg cggcgagagc
     1921 gagcgcctcg cgcccggccg cgaggctcct ctcgtccggc acggggtgcg ccgaggggag
     1981 gaagcgtccg ccgggcggaa gctcttcggg gaggactccg gccggggcga cgacgaccgc
     2041 ctcgtccacg aggtccccca tcgcccggag ggcggcggcc gccatcgccg gagccgcctt
     2101 cccgaccgcg aggaggacga cgcgccgggg gacgaagacg aactctcgcc ggccggggtc
     2161 ctcgaccgaa cggacgacga cggcgctgcc ccggcgggag agggcctccg acacgagcct
     2221 ccccggctcg acggccgcga ccgccgcgcg gtagagggcc ctgaggaagg agcgggggtc
     2281 caaggaggag aattctagcg aaccggggag cgccccgcct ctgcgagaat ccggccgggc
     2341 cccaccgggc aatggaggtg actcatgcct caggtcgacg tcgaccgaat cgtcggttcc
     2401 atccgcgacg gagcgggagc cgtccgcgag aagacccagg acgccgtgga ggacgtggcc
     2461 gacaagatcg aggacgccct cgagaaggcc gggaccgagg gtcggaagat ccagcgcgag
     2521 gtgaaggaag agctcgcgag gcggtggagg caggtcgacc gcgtcggccg cgagaacgcc
     2581 ttcctcatgg ccgccggtgc gctagccgtc ggtgtcctcg tcggctggct catctcccgc
     2641 gaccgccgcg gctgacgacc gcgtggacct cgtcgcacgg ttctcggagc tgaagtccgg
     2701 cttcgcccgc tccttctggg tcgcgaacgt cctcgagctc ttcgagcgct tctccttcta
     2761 cggctcgaag atcgtcctcg ccgtcttcct cgcggagacg gtgggcctcg ggccgttcgg
     2821 cgtcagcctc gtcgggctct acgggttcgc cgtctacttc ctcccgatcc tcgccggacc
     2881 cgtcgtcgac cgcttcggct tcaagaggag cctcgcggcc tgcttcgcga tcttctcctt
     2941 cggctacttc ctcatcggcc tctcggggat gccgatgggg cagccgctcg tccaggccgt
     3001 cgggacgaag acctgggtcg tctgcgccct gctgatcacg gccgtcggcg gctcgctcat
     3061 caagccctgc atcgtcggca ccgtcgcgcg gaccacgacg cacgagacga agtcgctcgg
     3121 ctactcgatc tactacacgc tcgtgaacat cggcggctgg ctcgggccgg tcctcgcgag
     3181 ccaggtgcgc gtctccctcg gcatcgcgca ggtcctcgtc ggggcctcgc tcgtctcgtt
     3241 cctcctcttc ctcgcgacgc tcgcgttctt caaggagccc gagcgtgacc ccgccgccga
     3301 ggggcggacg ctcgggaagg tcctccgcga cgcggggctc gtcctcgcga acggccgctt
     3361 catcggcttc ctcgtcatct tcaccggctt ctgggtgatg ttctggcagg tctacgacct
     3421 cttccccttc tacgtccgcg acgtcctgaa ggtggagcgg ttcgagttca tcgcctcgct
     3481 cgagtcgttc tcggtcatct tcctgaccgt cccggtcgcg gcgctgatga agaaggtccg
     3541 ggccgtgccg cagatgacgc tcggcctcgc ggtcgggagc tgctcctggc tcctcctcgt
     3601 cgtctcgcag acgtggcagg cggcggccgc ggcgatgttc ctcctcgccc tcggcgaggt
     3661 gctccaggcg ccgcgctact acgagtacat cgcggacctc gccccgaagg agcaggtcgg
     3721 cacgttcatg ggcttcgcct tcctcccgat cgccctcggc gccctcctcg cgggccccct
     3781 cggaggattc ctcctccagc actacctcaa ggacaccatg cagcccgcga aggcctggac
     3841 gatcctctcc tgcctcggct tcgtctcgac cgccgccctc ctcgcctacg accgctgggt
     3901 cgcccggcga tagaaacccg ggggtcaggt cttgcgatga ggcattcgcg atcgcgaatg
     3961 cctcatcgca agacctgacc cccggctact tgtcgtcggt gaggccccag gcgcggaggc
     4021 ggcggtggag ggtcgtgcgg tcgaggccga ggcgctcggc ggcgcgggag acgttgcccc
     4081 ggcactcctc cagcacccgg acgacgaggc ggcgctcgaa cgcgtcgcgc gcgtcggcga
     4141 gcgtctggcc cggcatcagg ccgtctc
//
LOCUS       DAOTEZ010000390         4164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_15727_length_4164_cov_6.620346, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000390 DAOTEZ010000000
VERSION     DAOTEZ010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15727_length_4164_cov_6.620346"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /locus_tag="P5164_16445"
     CDS             complement(<1..1196)
                     /locus_tag="P5164_16445"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRY45528.1"
                     /translation="MKRTAIERAREGRAADVPSRAGGKVLHILALSFLLPCARLLGEE
                     PLTDPRQWMVELEGESVIESWLKAGEAGRTSALGASVSRAAELERAQARVEELMEVPE
                     IGARVQYRTQRVFNGIAVFVDPSRVEKIRSLPGVKSVTPLVLHTPAVSTSVPFLHVPG
                     QVWQAHGNAGEGIRIGIIDSGIDYQHAAFGGSGTEADYKANDRTKAPDAFFPNARVVG
                     GYDFVGDEYGQGAPPKPDPDPMDCGGHGSHVAGIAAGSGVRVDGTTYPGPYDGTIPFS
                     SLRIGPGVAPKASLYALRVFGCSGGTGLVTQALEWAVDPNKDWDFSDRLDVVNLSLGS
                     VYGGAFDSAAKAADVAAKAGVVVVCAAGNAGDTHFVTGSPGSSDYSLSVAASVDSGVT
                     ASMLRVL"
     gene            1605..2261
                     /locus_tag="P5164_16450"
     CDS             1605..2261
                     /locus_tag="P5164_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRY45529.1"
                     /translation="MPRLRVASFSISIDGFGAGPDQGLENPLGFGGAQLHAWAYPTRT
                     FRRKVFGHDDGTTGLDDEFVARGFENVGAWILGRNMFGPVRGPWPDESWKGWWGEEPP
                     YHVPVFVLTHHARPPLEMEGGTTFHFVTGGIYEALERAREAAGERDVRLGGGPATIRQ
                     YLKEGLVDELHLAIAPVLLGAGEPLLAGLDLRALGYACTRHVASERATHVVLEGTPRR
                     "
     gene            2294..2743
                     /locus_tag="P5164_16455"
     CDS             2294..2743
                     /locus_tag="P5164_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005269398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRY45530.1"
                     /translation="MPSTILLHRVLKSPPDRVYRAFLDPDAMVKWLPPNGFTGKVHSM
                     DARVGGSHRMSFTNFSTGASHSFGGTYLELVPNEKLRYTDAFDDPSLPGGMEVTVTLK
                     KVSCGTELQITQEGIPDVIPPEACYLGWQESLVLLGKLVEAEIPDGA"
     gene            2817..3539
                     /locus_tag="P5164_16460"
     CDS             2817..3539
                     /locus_tag="P5164_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006528936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY45531.1"
                     /translation="MGVTNTASGTRVDEVADGIYRISTPFPAVPGGFSFNQYLVADDE
                     PLLFHTGPRTIFPLVREAIASVLPPERLRFVGYSHFESDECGSMPSLLALAPDAVPLS
                     GGVSAMINGDAFDRPPRALADGETLPLGRHRVRWIDAPHLPHAWECGYLFEETTATLF
                     CGDLFTQGGADNPPLTTSDILGPSEAFRRPMDYFSHARNVRTLLAPLVATRPATLACM
                     HGSAWRGDGARLLEALADALDA"
     gene            3583..>4164
                     /locus_tag="P5164_16465"
     CDS             3583..>4164
                     /locus_tag="P5164_16465"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HRY45532.1"
                     /translation="MDYARILAEIAEEVRPLVGQGRTADYIPPLADVPRDRFGMVLRT
                     VGNETFSVGHAQERFSIQSIAKVFSLTLATRLEGDAVWERVSREPSGTAFNSLVQLEV
                     ERGIPRNPFLNAGALVVLDMVLSHQREGEDLVLDFVRRAGANPAVGYDPVVAAAERAT
                     GHRNRALAHFLKSFGNLRNDPDRVLDAYFLLCSI"
BASE COUNT          613 a         1543 c         1394 g          614 t
ORIGIN      
        1 aggacccgga gcatggaggc cgtcacgccc gagtcgacgg aggccgcgac ggagagcgag
       61 tagtcgctgg aaccgggcga gcccgtcacg aagtgcgtgt ccccggcgtt ccctgccgcg
      121 cagacgacga cgaccccggc cttcgcggcg acatccgcgg ccttcgcggc gctgtcgaac
      181 gccccgccgt agacggagcc gagcgagagg ttgacgacgt cgagccggtc ggagaaatcc
      241 cagtccttgt tcgggtcgac ggcccactcg agcgcctggg tcacgaggcc cgtccctccc
      301 gagcagccga agacccggag cgcgtagagg ctcgccttcg gggcgacacc gggcccgatg
      361 cgcagcgagg agaaggggat cgtcccatcg tagggacccg gataggtcgt cccgtcgacc
      421 cgcacgcccg agccggcggc gattccggcc acgtggctcc cgtggcctcc gcagtccatc
      481 gggtcggggt cgggcttcgg cggcgcgccc tgcccgtact cgtccccgac gaagtcgtat
      541 ccgcccacga ccctcgcgtt cgggaagaag gcgtccggag ccttcgtccg atcgttggcc
      601 ttgtagtccg cttcggttcc gcttcccccg aacgcggcgt gctggtagtc gatgccgctg
      661 tcgatgatcc cgatccggat cccttccccc gcgttcccgt gcgcctgcca gacctggccg
      721 ggtacgtgga ggaaggggac gctcgttgaa acggccggag tgtggaggac cagaggcgtc
      781 accgacttca cacccggaag ggaacggatc ttctcgaccc gcgacgggtc gacgaagacc
      841 gcgatgccgt tgaagacccg ctgggtccgg tactggacgc gcgcgccgat ctccggcacc
      901 tccatcagct cctcgaccct ggcctgcgcc cgctcgagct ccgcagcgcg agagacggag
      961 gcgccgaggg ccgacgtccg ccccgcttcg ccggccttca gccagctctc gatcacgctc
     1021 tccccctcga gctcgaccat ccattggcgt gggtccgtca ggggctcctc gcccaggaga
     1081 cgggcacacg gcaggaggaa agacagggcc aggatatgca ggaccttccc gccggcccga
     1141 ctcggcacgt ccgcagcacg cccttcgcgc gccctctcga tcgcggttct cttcacttcg
     1201 atccgctcct tcgttctcga gacggctgga gtcgcgagct tctggggccc cggaggtcct
     1261 ggcttccgcg aaggccccgg cgtccgccct cctccgagcc gaagccggcg gctccctggt
     1321 gtcgtctcgc tcagagtacg tgctgctgca ccggcgactc tgcactcacg atgtgtgcca
     1381 cgcgatcgcg gcccctcacg aggacggtcg ggatcctttc gttcgtccct ctcatcgacg
     1441 ccgtccgccc ccgccaggtc cgcttcgccc cctgccagaa ggcgggcacg gcacgcctct
     1501 ccagtcaccg cctcctgaga tactctcgca acaaccagga cgacaggcgg cggtgggact
     1561 tcccggggac ggctccccgg cgggcacccc atggaggtga ggttgtgccg aggctgaggg
     1621 tcgcgagctt ctcgatctcg atcgacggct tcggcgcggg ccccgaccag ggcctcgaga
     1681 acccgctcgg gttcgggggc gcccagctcc acgcgtgggc ctacccgacc cggacgttcc
     1741 ggcggaaggt cttcgggcac gacgacggca cgacggggct cgacgacgag ttcgtcgcgc
     1801 gagggttcga gaacgtcggc gcgtggatcc tcggccggaa catgttcggc ccggtccgcg
     1861 gtccgtggcc cgacgagagc tggaaaggat ggtggggcga agagccgccc taccacgtcc
     1921 cggtcttcgt cctgacgcat cacgcccgtc cccccctcga gatggagggc gggacgacgt
     1981 tccacttcgt gacgggcggc atctacgagg cgctcgagcg ggcgcgcgag gccgcaggag
     2041 agcgggacgt ccgcctcggc ggcggaccgg caacgatccg gcaatacctg aaggaagggc
     2101 tcgtcgacga gctccacctc gccatcgccc ccgtcctcct cggcgccggg gagccgctcc
     2161 tcgcgggcct cgatctccgg gcgctcgggt atgcctgcac ccgccacgtc gcgtccgagc
     2221 gcgcgacgca cgtcgtcctg gaggggacgc cgcgccgctg agccgcgcgt ccgtcacgga
     2281 aaggaggccc gccatgccga gcacgatcct tctccaccgc gtcctcaagt ccccgcccga
     2341 ccgggtctac cgcgccttcc tcgaccccga cgcgatggtg aagtggctcc cgccgaacgg
     2401 cttcaccgga aaggtccact cgatggacgc gcgcgtggga ggctcccacc ggatgtcgtt
     2461 cacgaacttc tcgaccgggg cgagccactc cttcggcggc acctacctcg agctcgtccc
     2521 caacgagaag ctccggtaca cggacgcctt cgacgacccg agcctcccgg gcggaatgga
     2581 agtgaccgtg accctgaaga aggtctcctg cgggacggag cttcagatca cgcaggaagg
     2641 gatccccgac gtcatcccgc ccgaagcctg ctacctgggc tggcaggagt cgctcgtcct
     2701 tctcggaaag ctcgtcgagg ccgagatccc cgacggggcg tgagagcgat gacgcgcgag
     2761 agacctcgca cggacagagg cccggaccgc gcccgccgcg ggacgaagga ggaaacatgg
     2821 gcgtgacgaa caccgcttcc ggcacccggg tcgacgaggt cgcggacggc atctaccgga
     2881 tcagcacgcc gttccctgct gttcccggcg gcttctcttt caaccagtac ctcgtcgcgg
     2941 acgacgagcc gctcctcttc cataccgggc cgaggacgat cttcccgctc gtccgcgagg
     3001 cgatcgcgag cgtcctcccg ccggaacggc tccgtttcgt cggctactcc cacttcgagt
     3061 cggacgagtg cgggtcgatg ccgtccctcc tcgcgttggc cccggacgcg gtgccgctct
     3121 ccggaggggt gagcgccatg atcaacggcg acgccttcga ccggccgccg cgggccctcg
     3181 cggacggcga gacgctcccc ctcggtcggc accgcgtccg ctggatcgac gccccgcatc
     3241 tcccgcacgc gtgggagtgc gggtatctct tcgaggagac gaccgcgacg ctcttctgcg
     3301 gcgacctctt cacccagggg ggcgcggaca acccgcccct gacgacgtcc gacatcctcg
     3361 ggccgtccga ggccttccgg aggccgatgg actacttctc gcatgcgcgg aacgtccgca
     3421 cgctcctcgc gcccctcgtg gcgacacggc cggcgacgct cgcgtgcatg cacgggagcg
     3481 cgtggcgggg cgacggcgcg cggctcctcg aggcgctcgc cgacgccctg gacgcctgac
     3541 gcgcgaggcg accgacccgc ccgccgccgg taagatcgac ccgtggatta cgcgcggatc
     3601 ctcgccgaga tcgcagaaga ggtgcggcct ctcgtcgggc aggggcggac cgcggactac
     3661 atcccccctc tcgccgacgt cccgcgcgac cggttcggga tggtcctccg aacggtcggg
     3721 aacgagacct tctccgtggg tcacgcacag gagcggttct cgatccagag catcgccaag
     3781 gtcttcagcc tgacgctcgc gacgcggctc gagggagacg ccgtctggga acgcgtcagc
     3841 cgcgagcctt cgggaacggc cttcaactcg ctcgtccagc tcgaggtcga gcgcgggata
     3901 ccgaggaacc cgttcctcaa cgcgggggcg ctcgtcgtcc tcgacatggt cctcagccac
     3961 cagcgggagg gcgaggacct cgtgctcgac ttcgtccggc gcgccggggc gaaccccgcc
     4021 gtaggttacg accccgtggt ggccgcggcc gagcgggcga ccgggcaccg gaaccgcgcc
     4081 ctcgcccact tcctgaagtc gttcgggaac ctgaggaacg acccggaccg cgtcctcgac
     4141 gcctacttcc tcctctgctc gatc
//
LOCUS       DAOTEZ010000391         4155 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_15773_length_4155_cov_6.462683, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000391 DAOTEZ010000000
VERSION     DAOTEZ010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15773_length_4155_cov_6.462683"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="P5164_16470"
     CDS             <1..353
                     /locus_tag="P5164_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRY45533.1"
                     /translation="GARMQEGILSLMQMAKVSAALARLSEARVPYVTILTDPTTGGVT
                     ASFAMLGDVTLAEPKALIGFAGPRVIEQTIRQTLPEGFQRSEFLLDHGFVDVVVPRKE
                     LKATLANVLRLLAA"
     gene            350..1555
                     /locus_tag="P5164_16475"
     CDS             350..1555
                     /locus_tag="P5164_16475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRY45534.1"
                     /translation="MSPGAGLRWLDDLGPQKIRPGLSRTRALLASLGSPQASFRTLLV
                     AGTNGKGSTAATAAAVLEATGLRVGLTTSPHLVRVNERVRLPGGRDVSDEGLDSALSL
                     VAAVSGEGPLRPTYFEALVVAACELFRRARVEVAVVEVGIGGRLDATNVLDPEVSVVT
                     NVAADHLETLGPTLEDVAREKAGIFRPGRLALTTERGGTLGVLRAEAARVGATLVEVE
                     PSTEWESATPLVGAHQRENVALGVAAARALVPADDATVRRGVAAIRWPGRLQTVPRPG
                     ARPLLVDGAHNPAGAAAVAAYLDAAGLAGRVDLVFGALGDKAVEAMFAPLAERARRVV
                     LVAPDSPRAIPADALAARLGREELRRGASLAAALALLEGAEDDAPILVAGSLVLAGEA
                     LALVSRRDA"
     gene            1587..2951
                     /gene="dgt"
                     /locus_tag="P5164_16480"
     CDS             1587..2951
                     /gene="dgt"
                     /locus_tag="P5164_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HRY45535.1"
                     /translation="MSAGRPPQAVAYTKRELEAFEGERLAIYASRAAASTRRRPVAPE
                     ADGHDYRTEFQRDRDRIVHSKAFRRLKHKTQVFIPFEGDHYRTRLTHTLEVTQVARTV
                     ARTLGLNEDLAESCALAHDLGHTPFGHSGEKALNRILIGGEPSVSLSPKAVAAVGAFK
                     HNYQSVRVVDLLEKRYEEPGLNLTDETREGILKHTTWKRDFPFPVLDPEGLHLGSGCH
                     LEGQAVGWADEIAQQAHDLEDGLGLVGIGKAEEVPLAREIVAALGVAWRDASDPAVKR
                     ALLIRGLIHYLVTDLVVASRAAIETWLTAQKVTTRAEFAAHRKKLPGSLVAPSRGGEK
                     RYLELKEFVYRHIIHSQAVSQHDARAQLVVNRLFDAYAKNPRLLPDAVLARVAELSGL
                     PFLREVPLKKVEGVISGRWREEPAVLRCIADHIAGMTDSYALAEYDALFAPYPRRPAG
                     TPAG"
     gene            2961..3419
                     /gene="rpiB"
                     /locus_tag="P5164_16485"
     CDS             2961..3419
                     /gene="rpiB"
                     /locus_tag="P5164_16485"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HRY45536.1"
                     /translation="MRIAFGADHAGFELKTLLAGRAAELGHEVLDLGTSSTASVDYPD
                     FGYAVGEAVASGRADRGVVVCGTGIGISIAANRVRGARAGVATDLFSAQLMREHNDAN
                     VLGLGARVTAVPLATAILEAFLITPFAGGRHQRRVDKLDAPPPSAQGESR"
     gene            3416..>4155
                     /locus_tag="P5164_16490"
     CDS             3416..>4155
                     /locus_tag="P5164_16490"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRY45537.1"
                     /translation="MTTTQTPNPRRMGRPLAAVDPEIAAAVDAEFARQRDGLELIASE
                     NYASRAVLEATGSVLTNKYAEGYPGRRYYGGCENVDVAESLAIARAKKLFSPANPDAI
                     HANVQPHSGSQANTAVYLACLKPGDTLLGMSLAHGGHLTHGHPLSISGKQYRVVSYGV
                     TREAETIDYDELARIAEAEKPKLIIAGASAYPRVLDFPRFRQVADSVGALLMVDMAHI
                     AGLVAAGLHPSPVGHAHFVTSTTHKTLRG"
BASE COUNT          550 a         1648 c         1421 g          536 t
ORIGIN      
        1 gcggcgcgcg gatgcaggag gggatcctct ccctcatgca gatggcgaag gtctccgcgg
       61 cgctcgcgcg gctctccgag gcgcgcgtcc cctacgtgac gatcctgacc gacccgacga
      121 ccggcggcgt caccgcctcc ttcgccatgc tcggcgacgt cactctcgcc gagccgaagg
      181 ccctcatcgg gttcgcgggg ccgcgggtca tcgagcagac gatccggcag accctcccgg
      241 aaggattcca gcgctccgag ttcctcctcg accacggctt cgtcgacgtc gtcgtccccc
      301 gcaaggagct caaggcgacg ctcgcgaacg tccttcgtct cctcgccgcg tgagccccgg
      361 cgcgggcctc cgctggctgg acgacctcgg cccgcagaag atccggcccg gcctctcgcg
      421 cacacgggcg ctcctcgcct ccctcgggag cccgcaggcg tccttccgga ccctcctcgt
      481 cgccgggacg aacgggaagg gctcgaccgc ggcgacggct gcggcggtcc tcgaggcgac
      541 gggcctccgg gtcgggctca cgacctcgcc gcacctcgtc cgcgtgaacg agcgggtccg
      601 cctccccggc gggcgcgacg tctccgacga ggggctcgac tcggccctct ccctcgtcgc
      661 cgccgtctcg ggagaggggc ccctccgccc gacctacttc gaggcgctcg tcgtcgccgc
      721 ctgcgagctc ttccggcgcg cgcgggtcga ggtcgcggtc gtcgaggtag ggatcggcgg
      781 gcgcctcgac gcgacgaacg tcctcgaccc cgaggtctcc gtcgtcacga acgtcgcggc
      841 ggaccacctc gagacgctcg gtccgacgct cgaggacgtg gcgcgagaga aggcgggcat
      901 cttccggccg ggccgcctcg ccctcacgac ggagcggggc gggacgctcg gagtcctccg
      961 ggccgaggcg gcccgggtcg gcgcaaccct cgtcgaggtg gagccgtcga cggagtggga
     1021 gagcgccacg cctctcgtcg gcgcccacca gcgcgagaac gtcgccctcg gcgtcgcggc
     1081 ggcgcgcgcg ctcgtcccgg cggacgacgc gaccgtccgg cgcggcgtcg ccgcgatccg
     1141 ctggccgggg cggctccaga cggtcccgcg gccgggggcc cggccgctcc tcgtcgacgg
     1201 agcgcacaac ccggcggggg ccgccgccgt cgccgcgtac ctcgacgccg cgggcctcgc
     1261 agggcgcgtc gacctcgtct tcggggccct cggcgacaag gccgtcgagg cgatgttcgc
     1321 gccgctcgcc gagcgcgccc gccgcgtcgt cctcgtcgcc ccggattcgc cccgcgcgat
     1381 cccggccgac gccctcgccg cccggctcgg gcgggaggag ctccggcgcg gcgcctcgct
     1441 cgccgcggcg ctcgccctcc tcgagggggc ggaggacgac gcccctatac tcgtcgccgg
     1501 gtcgctcgtc ctcgccggtg aggctctggc gctcgtctcg cgccgggacg cctgagccgt
     1561 cggcgggcgg acgcccggag gttcggatga gcgcagggag gcccccgcag gccgtcgcct
     1621 acacgaagcg cgagctcgag gcgttcgagg gcgagcgcct cgcgatctac gcctcccggg
     1681 cggcggcctc gacgcgccgc cgcccggtcg ctcccgaggc ggacggccac gactaccgga
     1741 ccgagttcca gcgcgaccgc gaccggatcg tccactccaa ggcgttccgg cgcctgaagc
     1801 acaagacgca ggtcttcatc ccgttcgagg gggaccacta ccggacgcgc ctgacgcaca
     1861 cgctcgaggt gacgcaggtc gcgcggacgg tggcgcgcac cctcggcctc aacgaggacc
     1921 tcgcggaatc gtgcgccctc gcgcacgacc tcggccacac gccgttcggc cactccggcg
     1981 agaaggcgct gaaccggatc ctgatcgggg gcgagccgag cgtctcgctc tctccgaagg
     2041 ccgtggccgc cgtcggcgcg ttcaagcaca actaccagtc ggtccgcgtc gtcgacctcc
     2101 tcgagaagcg gtacgaggag ccggggctga acctgaccga cgaaacgcgc gaggggatcc
     2161 tcaagcacac cacctggaag cgcgatttcc cgttcccggt cctcgacccg gagggcctcc
     2221 acctcggctc gggctgccat ctcgaggggc aggcggtcgg ctgggccgac gagatcgccc
     2281 agcaggcgca cgacctcgag gacggcctcg gcctcgtcgg gatcgggaag gccgaggagg
     2341 tccccctcgc gagggagatc gtggcggcgc tcggcgttgc gtggcgcgac gcttccgacc
     2401 cggccgtgaa gcgggcgctc ctgatccgcg gcctgatcca ctacctcgtc accgacctcg
     2461 tcgtcgcctc gcgcgcggcg atcgagacgt ggctgacggc gcagaaggtg acgacgcggg
     2521 ccgagttcgc ggcccaccgt aagaagctcc ccggctccct cgtcgccccc tcgcgcggcg
     2581 gggagaagcg gtacctcgag ctgaaggagt tcgtctaccg ccacatcatc cacagccagg
     2641 ccgtctcgca gcacgacgcg cgcgcccagc tcgtcgtcaa ccgcctcttc gacgcctacg
     2701 cgaagaaccc caggctcctc cccgacgccg tcctcgcgcg cgtcgcggag ctctcgggcc
     2761 tccccttcct ccgggaggtg ccgctcaaga aggtcgaagg ggtcatctcc gggcgctggc
     2821 gcgaggagcc ggcggtcctt cgctgcatcg ccgaccacat cgccggcatg acggacagct
     2881 acgccctcgc ggagtacgac gccctcttcg ctccctaccc gaggaggccg gcggggacgc
     2941 ccgcggggta ggatcggccg atgcgcatcg ccttcggcgc cgaccacgcc ggcttcgagc
     3001 tgaagaccct cctggccggg cgggccgcgg agctcggcca cgaggtgctc gacctcggga
     3061 cctcttccac cgcctccgtc gactaccccg acttcggcta cgccgtcggc gaggccgtgg
     3121 cgtccggacg ggccgaccgc ggcgtcgtcg tctgcggcac cgggatcggc atctccatcg
     3181 cggcgaaccg cgtgcggggc gcgcgggccg gcgtcgcgac cgacctcttc tccgcgcagc
     3241 tgatgcgaga gcacaacgac gcgaacgtcc tcggcctcgg cgcccgcgtc accgccgtcc
     3301 cgctcgcgac ggcgatcctc gaggccttcc tgatcacccc gttcgccggc gggcggcacc
     3361 agcggcgcgt cgacaagctc gacgcccccc cgccgagcgc ccaaggagag tcccgatgac
     3421 gacgacccaa accccgaacc cccgacggat ggggcggccc ctcgccgccg tcgaccccga
     3481 gatcgccgcg gccgtcgacg ccgagttcgc ccgccagcgc gacggactcg agctgatcgc
     3541 ctcggagaac tacgcctccc gcgccgtcct cgaggcgacg ggctccgtcc tgacgaacaa
     3601 gtacgccgaa ggctacccgg gccgccgcta ctacggcggc tgcgagaacg tcgacgtcgc
     3661 cgagtcgctc gcgatcgctc gcgcgaagaa gctcttctcg cccgcgaacc ccgacgcgat
     3721 ccacgcgaac gtccagcccc actcgggctc gcaggcgaac acggccgtct acctcgcgtg
     3781 cctgaagccg ggcgacacgc tcctcggcat gtccctcgcg cacggcggcc acctgacgca
     3841 cggccacccg ctctcgatct cgggaaagca gtaccgcgtc gtctcctacg gcgtcacgcg
     3901 cgaggccgag acgatcgact acgacgagct cgcccggatc gccgaggccg agaagccgaa
     3961 gctgatcatc gcgggggcct cggcctaccc gcgcgtcctc gacttcccgc gcttccggca
     4021 ggtcgccgac tccgtcggag cgcttctcat ggtcgacatg gcccacatcg ccggcctcgt
     4081 cgcggcgggc ctccacccgt cgcccgtcgg ccacgcccac ttcgtcacgt cgacgacgca
     4141 caagacgctc cgcgg
//
LOCUS       DAOTEZ010000392         4144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_15834_length_4144_cov_7.448520, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000392 DAOTEZ010000000
VERSION     DAOTEZ010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15834_length_4144_cov_7.448520"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="P5164_16495"
     CDS             <1..855
                     /locus_tag="P5164_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013665962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY45538.1"
                     /translation="TRLYWRAGWRLLFSRRFPAYLAELARRLVDELALQRFIWKRSPK
                     RGLAHVLLAWGCLSAFAITIPLVFGWVHFTLKSGTIDVYEAHLLGFHAFDFPLGGFVA
                     LNLFHALNWSSVLVLLGVFGFLRRRITDAGQISTQTFEMDLMPLVLLVLISVTGLGLT
                     WDYELMQGRAHAFMAISHAITVILFLLWLPFGKFFHVFQRPAQLGVALYQREGEAGAQ
                     AVCPHTGEAFASRLHVEDLKQVTSELGYDYRLTGGGSHLDLSPQGKRAALAKAHLAAR
                     SESGAFFG"
     gene            870..>2266
                     /locus_tag="P5164_16500"
     CDS             870..>2266
                     /locus_tag="P5164_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRY45539.1"
                     /translation="MARLPVSPEALVAQYGPRLGFPPKEGFPGRDEPDRVVPTHCCFC
                     GMQCGIKLLVKDNRVVGFDPWEEFPFNEGRLCPKGVQRYLQNNHPDRLLSPLERVEGV
                     GFRPVAWDAALDRTASEIRRIQEKHGKDAFAMLSGVSLTNEKSYLVGKFARLAIGTRN
                     LDYNGRLCMVSAGAGNKKAFGLDRASNTYADLAKAEVILVAGANVSETFPTLTHWIWQ
                     ARDNGAKLIVVDPRMTPLARTADLHLPVRPGRDSALFGAILKQLVDRDQLDHDFIANH
                     TSGFEEAVEAVKGYDLAWAEEVTGIPRRKIEAAAEWWGTAKTSFLLHARGIEHHTKGV
                     ENVVSAINLVLATGRIGKPYCGYGTITGQGNGQGGREHGHKCDQLPGNRDIENPKHRE
                     WIAGVWGVPEPEIPGKGLSAYEIVEAIHRGEIKGLLSICFNPLVSLPNASFVREALEK
                     LEYFAAIDFFLSETAR"
     assembly_gap    2267..2366
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2367..3283
                     /locus_tag="P5164_16505"
     CDS             <2367..3283
                     /locus_tag="P5164_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HRY45540.1"
                     /translation="EKLEYFAAIDFFLSETARHADVVLAGSLHEEEEGTVTTAEGRVT
                     RVRAAIDPPADAWRDTDVLLDLARRLGKGELFDFSAPAPLAGTREGSLFTARRIFDEL
                     RAASKGGTADYFGITYEKIEANLGVFWPCPSLDHPGTPRLWEDRKFATPDGKAHFNAV
                     RYRDPGEVTDPDYPVILTTGRVVSQYLSGTQTRRIGKLVEQYPEPLLEIHPSLAKSLG
                     IADRDLVRVETRRGAAEFPAQLVTTIREDTVFIPYHWPGRKSANLLTSGLLDPVSKIP
                     EFKVSACRLVPTGRKAPSAAEAKAHASV"
     gene            3297..3881
                     /locus_tag="P5164_16510"
     CDS             3297..3881
                     /locus_tag="P5164_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRY45541.1"
                     /translation="MTTTAYRSQEEFFLDPQRCIGCHACEMACAECETNGQLAMIHVD
                     YVDRAESPQTSVQVCMHCEEPTCARVCPADAIAKDELGVVHSANAARCIACSNCVLAC
                     PFGVPKKVEPMKLMMKCNLCYDRTSAGRRPMCATVCPSGALWYGTRERMAAMRPNSTP
                     VNEFRFGLQRVKTRVNVMMPKGSTELVVDGTEAP"
     gene            3878..>4144
                     /locus_tag="P5164_16515"
     CDS             3878..>4144
                     /locus_tag="P5164_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45542.1"
                     /translation="MSDRPDGLPARPAWEVDFPLERVEAQHVTRREFAKFLVLASGGL
                     AVGSGWVAAKDALFPPATIPGPVRLASLSEIPVGGMTQFTIPGLD"
BASE COUNT          623 a         1492 c         1347 g          582 t
ORIGIN      
        1 acgcggctct actggcgggc cgggtggcgg ctcctcttct cccgccgctt ccccgcctac
       61 ctcgccgagc tcgcccgccg cctcgtcgac gagctcgcgc tccagcgctt catctggaag
      121 cggagcccga agaggggcct cgcgcacgtc ctcctcgcgt gggggtgcct ctcggccttt
      181 gcgatcacga tcccgctcgt cttcggctgg gtccacttca cgctgaagag cggcacgatc
      241 gacgtctacg aggcgcacct cctcggcttc cacgccttcg acttcccgct cggcggcttc
      301 gtcgcgctga acctcttcca cgccctgaac tggtcgtcgg tcctcgtcct cctcggagtc
      361 ttcgggttcc tccgtcgccg gatcaccgac gccgggcaga tctcgacgca gaccttcgag
      421 atggacctga tgccgctcgt cctcctcgtc ctgatctccg tgacggggct cggcctgacg
      481 tgggactacg agctgatgca ggggcgggcc cacgccttca tggcgatcag ccacgcgatc
      541 acggtgatcc tcttcctcct ctggctcccc ttcgggaagt tcttccacgt cttccagcgc
      601 ccggcgcagc tcggcgtcgc cctctaccag cgcgagggcg aggcgggcgc gcaggccgtc
      661 tgcccgcaca ccggcgaggc cttcgcgtcg cggctgcacg tggaggacct caagcaggtg
      721 acgtcggagc tcggctacga ctaccggctc acgggcggcg gctcgcacct cgacctctcc
      781 ccgcagggaa aacgggccgc gctcgcgaag gcccacctcg ccgcccgcag cgagtcgggc
      841 gccttcttcg gctgagagga gcgaacgaaa tggcacgcct ccccgtctcc cccgaggccc
      901 tcgtcgcgca gtacgggccg cgcctcggct tcccgccgaa ggaggggttc cccggccgcg
      961 acgagcccga ccgcgtcgtc ccgacccact gctgcttctg cgggatgcag tgcggcatca
     1021 agctcctcgt caaggacaac cgcgtcgtcg gcttcgatcc gtgggaggag ttcccgttca
     1081 acgaggggcg cctctgcccg aagggggtcc agcggtacct ccagaacaac caccccgacc
     1141 ggctcctctc tcccctcgag agggtcgagg gagtcgggtt ccgccccgtc gcgtgggacg
     1201 ccgccctgga ccgcaccgct tccgagatcc ggcggatcca ggagaagcac gggaaggacg
     1261 ccttcgccat gctctcgggc gtctcgctga cgaacgagaa gagctacctc gtcgggaaat
     1321 tcgcccgcct cgcgatcggg acgcggaacc tcgactacaa cggccggctc tgcatggtga
     1381 gcgccggggc cgggaacaag aaggccttcg gcctcgaccg ggcgagcaac acgtacgccg
     1441 acctcgcgaa ggccgaagtg atcctcgtcg ccggcgcgaa cgtcagcgag acgttcccga
     1501 cgctcacgca ctggatctgg caggcgcgcg acaacggggc gaagctgatc gtcgtcgacc
     1561 cgcggatgac gccgctcgcg cggacggccg acctccacct ccccgtccgc cccggacgcg
     1621 actcggccct cttcggggcg atcctcaagc agctcgtcga ccgcgaccag ctcgaccacg
     1681 acttcatcgc gaaccacacc tccggattcg aggaggccgt cgaggcggtg aaggggtacg
     1741 acctcgcctg ggccgaggag gtcacgggga tcccgcggcg gaagatcgaa gccgcggcgg
     1801 agtggtgggg gacggcgaag acgagcttcc tcctccacgc gcgcggcatc gagcaccaca
     1861 cgaagggggt cgagaacgtc gtctcggcga tcaacctcgt cctcgcgacg gggcggatcg
     1921 ggaagcccta ctgcggctac gggacgatca ccggccaggg gaacgggcag ggggggcgcg
     1981 agcacggaca caagtgcgac cagctccccg gcaaccgcga catcgagaac ccgaagcacc
     2041 gcgagtggat cgccggcgtc tggggcgtgc cggagccgga gatcccgggg aaggggctct
     2101 cggcctacga gatcgtcgag gcgatccacc ggggcgagat caaggggctc ctctcgatct
     2161 gcttcaaccc gctcgtctcg ctcccgaacg cgagcttcgt ccgcgaggcg ctcgagaagc
     2221 tcgagtactt cgccgcgatc gacttcttcc tctccgagac ggcccgnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnntcga gaagctcgag tacttcgccg cgatcgactt
     2401 cttcctctcc gagacggccc ggcacgccga cgtcgtcctc gccgggtcgc tccacgagga
     2461 ggaggaaggg acggtcacga cggccgaggg gcgcgtgacg cgcgtgaggg ccgcgatcga
     2521 cccgcccgcg gacgcctggc gcgacaccga cgtcctcctc gacctcgccc gccgcctcgg
     2581 gaagggcgag ctcttcgact tctccgcgcc ggccccgctc gccgggacac gggagggctc
     2641 gctcttcacg gcgaggcgga tcttcgacga gctccgcgcc gcctcgaagg gaggaacggc
     2701 cgactacttc ggcatcacct acgagaagat cgaggcgaac ctcggcgtct tctggccctg
     2761 cccgtccctc gaccaccccg gcacgccccg cctctgggag gaccggaagt tcgcgacgcc
     2821 ggacgggaag gcgcacttca acgccgtccg gtaccgcgac ccgggcgagg tgaccgaccc
     2881 cgactacccc gtgatcctga cgacggggcg cgtcgtctcg cagtacctct cggggacgca
     2941 gacccgtcgc atcggcaagc tcgtcgagca gtatccggag ccgctcctag agatccaccc
     3001 gtcgctcgcg aagagcctcg ggatcgccga ccgcgacctc gtgagggtcg agacgcggcg
     3061 cggcgccgcc gagttcccgg cgcagctcgt gacgacgatc cgcgaggaca ccgtcttcat
     3121 tccgtaccac tggccgggcc ggaagtcggc gaacctcctc acgtcgggcc tcctcgatcc
     3181 ggtctcgaag atccccgagt tcaaggtgag cgcctgccgg ctcgtgccga cgggccgcaa
     3241 ggccccttcc gccgccgagg cgaaggcaca cgcgagcgtc tgaggcggga agagcgatga
     3301 cgacgaccgc ctaccgaagc caggaggagt tcttcctcga cccgcagcgc tgcatcgggt
     3361 gccacgcctg cgagatggcc tgcgccgagt gcgagacgaa cggccagctc gcgatgatcc
     3421 acgtcgacta cgtcgaccgc gccgagagcc cgcagacgtc ggtccaggtc tgcatgcact
     3481 gcgaggagcc gacctgcgcc cgcgtctgcc ccgccgacgc gatcgcgaag gacgagctcg
     3541 gcgtcgtcca ctcggcgaac gccgcccgct gcatcgcctg cagcaactgc gtcctcgcct
     3601 gcccgttcgg cgtcccgaag aaggtggagc cgatgaagct gatgatgaag tgcaacctct
     3661 gctacgaccg gacgagcgcg gggcgaaggc cgatgtgcgc gacggtctgc ccgagcgggg
     3721 cgctctggta cggcacgcgc gagcggatgg ccgccatgcg cccgaacagc acgcccgtga
     3781 acgagttccg gttcggcctg cagcgggtga agacgcgcgt gaacgtgatg atgccgaagg
     3841 gctcgaccga gctcgtcgtc gacggaacgg aggcgccatg agcgaccgcc ccgatggcct
     3901 ccccgcccgc cccgcgtggg aggtcgactt ccccctcgag cgcgtcgagg cgcagcacgt
     3961 cacgcggcgc gagttcgcga agttcctcgt cctcgcctcc ggcggcctcg cggtcgggag
     4021 cggctgggtc gccgcgaagg acgctctctt cccgcccgcg acgatcccgg gaccggtccg
     4081 cctcgcgtcc ctctccgaga tcccggtcgg cgggatgacg cagttcacga tcccgggcct
     4141 cgac
//
LOCUS       DAOTEZ010000393         4144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_15835_length_4144_cov_6.159452, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000393 DAOTEZ010000000
VERSION     DAOTEZ010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15835_length_4144_cov_6.159452"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1267)
                     /locus_tag="P5164_16520"
     CDS             complement(<1..1267)
                     /locus_tag="P5164_16520"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRY45543.1"
                     /translation="MSERPLSVHKLGGTSLGDAERIVRAARLLAGASRRRRVVAVASA
                     MGGATDLLLRAAHEAERGRLRPALRSVAELRSRHEAAAAGIAAATGASVDFGLAPFVE
                     LETALSGIALLRERTPRVIDLVASFGERLSVPLLAAALEATGVPARAVDARTFLVTDA
                     AHGAATCDRRRTDPKARRLLGRLLAEGVVPVVTGFVGRTPSGETTTLGRSGSDTTAAL
                     LGAALGAREVVIWTDVDGVMTADPRIVPGARLLPRLSYREAAEMTYFGAKVLHFPAVL
                     PAVAARIPLVIRSSFAPDGPGTTISEKGDPRPGIRAVTAVSGLCLLSLDGKGMTGVPG
                     IAARVFQTTARLGVSVVMFSQASSEQNIAIVLPEADRARTAAALVQEFRYEKLVGAID
                     GVAAKSPIAVVAAVGDGMRGTVGIAAKVFA"
     gene            complement(1260..2204)
                     /locus_tag="P5164_16525"
     CDS             complement(1260..2204)
                     /locus_tag="P5164_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45544.1"
                     /translation="MSRPPRIELPGALHHVSWRALERSPLFRDDVDRERFLADLGRTA
                     SERGWIVHAYALLGSSVEVLVETPAPNLSPGMRALGGRYAQAFNRRHGRGGPLFDGRF
                     RSLLIDPGTAFLDAVRVVALAPVRARLARAALDWRWSSAPATAGLAERPSWLTVDATL
                     RRLSPARPRARERYRRLVSDTRNIRPLRERAVSRVVVGSAEFAREVRIRLAAPLPPSD
                     RPRPGAVELSRVRAAVASRFGVPEPALVRAGAHEAKVAAIWLSRRLTGLSGREVGEAF
                     GVRPARVSNVLGDVRTGRWRALRPTLEEMEKRLLAENE"
     gene            2291..>4144
                     /locus_tag="P5164_16530"
     CDS             2291..>4144
                     /locus_tag="P5164_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45545.1"
                     /translation="MANERAGEVRCEAPHGWAEAPARRAAPDPLARRLTEGARLDLLR
                     TFRLAPRPGAPDGTARVVLPARRGEDLLVVLRQPSGALSIHRPVGATARRGSGEVVFE
                     LPLRRPPGRRGPAGEAVVLVLRAAARVEARAAATLARTFEEAAFRRAGLAEGLVHVSP
                     EGLRDGHLAPASPALFAPPPARNLLLLHGTFSSTASAFADLARSGFFAALEPIYGERI
                     VGFDHFTLSRTPAENARALAAALPGEEPFLFDVVAHSRGGLALRTLVERPEEVGPVAS
                     RIATGRTVLAGVPNLGTPLASPDRWELALSGWLNLVDLLPKAPAPLLAAFVVEALLWL
                     ARRVTTLLPGLSAMDPAGETVAALGKRALPGAYSALAARHEATGAVARRLLDAGADRF
                     FGEPNDLVVPTEGCFVLDRSSGARVPAERVALLGSGGSARPVPAHHFDLFSRDEAIAF
                     LAATLGGEPAAHAGAEERRRGRAAASARVADEGAKPVPRAARRAAAPRIGEARREALE
                     LTLLPGDRRDLTALLFARYGTARVVAEVPRKGEAWREIIRGHERLRAHFDGDGRPLPS
                     EAALERWGAVLFETLFPGEVRRLWDAARGAASGGATDVVFTSLLDWVADKPW"
BASE COUNT          485 a         1551 c         1641 g          467 t
ORIGIN      
        1 cggcgaagac cttcgccgcg atgccgacgg tgccgcgcat cccgtccccg acggcggcga
       61 cgacggcgat cggcgacttc gccgccacgc cgtcgatcgc gccgacgagc ttctcgtatc
      121 ggaattcctg cacgagggcc gccgcggttc gcgcgcggtc ggcctcgggc aggacgatgg
      181 cgatgttctg ctcggacgac gcctgggaga acatgacgac ggagacgccg agccgcgccg
      241 tcgtctggaa gacgcgcgcc gcgatccccg gcacgcccgt catccccttc ccgtcgagcg
      301 agaggaggca gaggccggag acggcggtga ccgcgcggat gccggggcgg gggtccccct
      361 tctccgagat cgtcgtcccc ggcccgtccg gtgcgaagga gctccggatg acgagcggga
      421 tccgcgcggc gaccgccggg aggaccgccg ggaagtggag caccttcgcg ccgaagtagg
      481 tcatctccgc ggcctcgcgg taggagaggc gcggcaggag gcgggccccg gggacgatcc
      541 gggggtcggc cgtcatcacg ccgtcgacgt cggtccagat gacgacctcg cgcgccccga
      601 gcgccgcgcc gaggagggcg gccgtcgtgt cggagccgga gcggccgagc gtcgtcgtct
      661 cgccggacgg cgtccgcccg acgaagccgg tgacgaccgg gacgaccccc tcggccagga
      721 ggcggccgag caggcggcgc gccttcgggt cggttcgcct ccggtcgcac gtcgccgcgc
      781 cgtgggcggc gtcggtgacg aggaacgtcc gggcgtcgac ggcgcgggcg gggacgcccg
      841 tcgcctcgag ggcggcggcg aggagcggca ccgagaggcg ctcgccgaac gaggcgacga
      901 gatcgatgac gcgcggcgtt cgctcgcgca ggagcgcgat ccccgagagc gccgtctcga
      961 gctcgacgaa aggggccagg ccgaagtcga cggacgctcc ggtcgccgcc gcgatcccgg
     1021 ccgcggcggc ctcgtgccgg gacctcagct cggcgacgct ccggagcgcc gggcggagcc
     1081 gccccctttc ggcctcgtgc gcggcgcgca ggaggaggtc ggtggcgccg cccatcgcag
     1141 aggcgaccgc gacgacacgg cggcggcggg aggcgccggc gaggaggcgc gcggcccgga
     1201 cgatccgctc cgcgtcgccg agcgaggtgc cgccgagctt gtggacggag agggggcgct
     1261 cactcatttt ccgcgaggag cctcttctcc atctcctcga gcgttggacg gagcgccctc
     1321 cagcgccccg tccggacgtc gccgaggacg ttgctgaccc gcgcggggcg gacgccgaag
     1381 gcctctccca cctcgcgtcc cgagaggccc gtgaggcggc gcgagagcca gatcgcggcc
     1441 accttcgcct cgtgcgcgcc ggcccggacg agggccggct cggggacgcc gaaacgcgaa
     1501 gcgaccgcgg ccctcacgcg cgaaagctcg acggcgccgg ggcgcggccg atcggatggc
     1561 gggagcggcg cggcgaggcg gatccggacc tcgcgcgcga actccgcgct cccgacgacg
     1621 acgcgcgaga cggcccgctc gcgaagcggc cggatgttgc gcgtgtcgga gacgagccgc
     1681 cggtagcgct cgcgggcgcg cggccgggcc ggggagaggc gccggagcgt ggcgtcgacg
     1741 gtgagccagc tcgggcgctc ggcgagaccg gcggtggcgg gcgcgctcga ccagcgccag
     1801 tcgagcgcgg cccgcgcgag ccgggcccgc acgggggcga gggcgacgac gcggacggcg
     1861 tcgaggaaag cggtcccggg gtcgatcagg agcgagcgga agcgcccgtc gaagagcggc
     1921 ccgccccggc cgtgtcgccg gttgaaggcc tgcgcgtagc ggccgccgag ggcgcgcatc
     1981 ccgggggaga ggttcggagc gggcgtctcg acgaggacct cgacggagct cccgaggagc
     2041 gcgtaggcgt ggacgatcca gccccgttcc gacgcggtcc gcccgaggtc ggcgaggaaa
     2101 cgctcccggt cgacgtcgtc ccggaagagc gggctccgct cgagcgcccg ccaggagacg
     2161 tgatgcaggg cccccgggag ctcgatgcgg ggcggacgcg acatgtgccg cgattgtaga
     2221 ggcggggggc cccgcctccg cggcgcacct gcgccggggg cggtacgatg ccgccttcgg
     2281 gagacggccg atggcgaacg aacgcgcggg cgaggtgcgg tgcgaggcgc cgcacgggtg
     2341 ggcggaggcg cccgcccgcc gcgcggcgcc cgacccgctt gctcgccggc tgacggaggg
     2401 agcgcgactc gacctccttc ggacgttccg gctcgcgccc cggccgggag ccccggacgg
     2461 gacggcgcgg gtcgtcctgc cggcgcgccg cggagaggac ctcctcgtcg tcctccgtca
     2521 gccatcgggc gcgctctcga tccaccggcc ggtcggcgcg acggcgcggc gcggctccgg
     2581 cgaggtcgtc ttcgagctgc cgctccgacg gccgcccggg cggcgcggcc cggccggcga
     2641 ggccgtcgtc ctcgtcctcc gtgcggccgc gcgcgtcgag gcgcgtgcgg ccgcgacgct
     2701 ggcccggacg ttcgaggagg cggccttccg gcgcgcaggc ctcgccgagg ggctcgtcca
     2761 cgtgagcccg gaagggctgc gggacggcca tctcgcgccc gcgtctcccg cgctcttcgc
     2821 gccgccgccg gcccggaacc tcctcctcct ccacgggacg ttctcgagca cggcctcggc
     2881 gttcgccgat ctcgcccgga gcggcttctt cgccgcgctc gagccgatct acggagaacg
     2941 catcgtcggc ttcgaccatt tcacgctgag ccggacgccc gccgagaacg cgcgcgcgct
     3001 cgccgcggcg ctgcccggag aggagccgtt cctcttcgac gtcgtcgccc actcccgcgg
     3061 cggccttgcc ctgcgaacgc tcgtcgagcg gcccgaggag gtcggccccg tcgcgagccg
     3121 gatcgccacg ggccggaccg tcctcgcggg ggtgccgaac ctcgggacgc cgctcgcctc
     3181 gcccgaccgc tgggagctcg ccctctcggg ctggctgaac ctcgtcgacc tgctgccgaa
     3241 ggcgcccgcg ccgctcctcg cggccttcgt cgtcgaggcg ctcctctggc tcgcgcggcg
     3301 cgtgacgacg ctccttccgg gcctctcggc gatggacccg gcgggcgaga cggtcgccgc
     3361 cctcgggaag cgcgcgctcc cgggagccta ctcggccctc gccgcgcggc acgaggcgac
     3421 gggggcggtc gcgcgccgcc ttctggacgc gggagccgac cgcttcttcg gcgagccgaa
     3481 cgacctcgtc gtcccgaccg agggctgctt cgtcctcgac cggtcgagcg gcgcccgcgt
     3541 cccggcggag agggtcgccc tcctcgggtc gggcgggtcc gcgcggcccg tgcccgcgca
     3601 ccacttcgac ctcttctctc gcgacgaggc gatcgcgttc ctcgccgcga cgctcggggg
     3661 cgagccggca gcccacgccg gagcggagga gcgccgtcgc ggccgagcgg ctgcttccgc
     3721 ccgcgttgcg gacgagggag cgaagcccgt gccccgcgcg gcacgccggg ccgcggcccc
     3781 ccggatcgga gaagcacggc gcgaggcgct cgagctgacg ctcctgccgg gcgaccggcg
     3841 cgacctgacg gcgctcctgt tcgcccggta cggcacggcc cgggtcgtgg ccgaggtgcc
     3901 gcggaaggga gaggcctgga gggagatcat ccgcgggcac gagcgcctcc gcgcccactt
     3961 cgacggcgac ggacggcctc tgccgtccga ggccgcgctc gagcgctggg gcgccgtcct
     4021 cttcgagacg ctcttccccg gggaggtccg gaggctctgg gacgcggcgc gcggggccgc
     4081 atcgggcggg gcgaccgacg tcgtcttcac gtcgctcctc gactgggtgg ccgacaagcc
     4141 gtgg
//
LOCUS       DAOTEZ010000394         4132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_15893_length_4132_cov_6.557027, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000394 DAOTEZ010000000
VERSION     DAOTEZ010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15893_length_4132_cov_6.557027"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..838)
                     /gene="pgi"
                     /locus_tag="P5164_16535"
                     /pseudo
     CDS             complement(1..838)
                     /gene="pgi"
                     /locus_tag="P5164_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941965.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; incomplete;
                     partial in the middle of a contig; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
     gene            1036..2013
                     /locus_tag="P5164_16540"
     CDS             1036..2013
                     /locus_tag="P5164_16540"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRY45546.1"
                     /translation="MADSRQLVLGIDVGGTKVLAGAVDEGGRVLGRGKVKSPFRGGPE
                     EMTGALVAAADAALAEAGARRADVAALGLGVPGPLDAERTTLLRAMNLGVERWPVAAS
                     LEPHFPGVPKRLENDVRAAALGEARFGAAKGERLVVAIWVGTGIGGAVLLDGDLWLGR
                     NRNAGEIGQSFVDLKKAGSFDGTLEGIGAKVGMTAFLRRRIERGEKTVVARAVLEKDG
                     RLKGSEVRKALAAGDALVERALARSARAVGVAIGTLWNVFSPDLFVLGGGIAEDVGEP
                     YVEQVRAAAGRYAFFTDLAEVRVVRSALGDDAGILGGAVLAREGHRSRG"
     gene            2083..2877
                     /gene="ricT"
                     /locus_tag="P5164_16545"
     CDS             2083..2877
                     /gene="ricT"
                     /locus_tag="P5164_16545"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HRY45547.1"
                     /translation="MSCATCSIGTLVNPGGNYVGARLADATDGKVTVVFTGEVAVRPG
                     ERIVVETEEGPEFAEVTTLSPVLAKACSQKKAKRFLRLASDSESADFAERLELQDEAA
                     RWASKRIAELRLPVSLVKCVVPFDRRRITLLYSSDERANLRDLCREMSDRFHLRVESR
                     MVGVRDETKIVGGIGPCGLTLCCSTWLKGFHPVTIRMAKLQGITPNPSKLTGMCGRLK
                     CCLAYELEGGIEAARKMGSKGPDRKDADRKAPPAPPVPPAPVPPAA"
     gene            2951..3376
                     /locus_tag="P5164_16550"
     CDS             2951..3376
                     /locus_tag="P5164_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HRY45548.1"
                     /translation="MAAVRKPPSPASPAGAREPESELGPRKLIRPALSDLRRDMGPRG
                     LRKGAPPEQTNAESFYYLKQMQARTPVVVLLVDGEELRGWIEWYDKDAIKLNREHAPN
                     LLLQKHAIKYLYKEEEERPFRRRKVRSEPDGAPPEAGGR"
     gene            3433..>4132
                     /locus_tag="P5164_16555"
     CDS             3433..>4132
                     /locus_tag="P5164_16555"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRY45549.1"
                     /translation="MKTEKLTREALTAYAAENREAFEALLKDFVEIPSVSVDPTKKSA
                     IRENAKLAADVIRRFGGESRILETDGHPIVAGAWGKNPLWPTVTVYNHLDVQPASIET
                     EPWKTEPFTFTKQGDRYFGRGTTDDKGPALTALYGIKAALDAELPVNLRVLWEFEEEI
                     GSPSFEKAIAAHADLLATDSVLVSDTIWIARGKPACPAGLRGLQGFLFTLETGETDQH
                     SGVCGGAARNPMAEL"
BASE COUNT          618 a         1456 c         1537 g          521 t
ORIGIN      
        1 actccatgac gagctgctgg aggtagcggg agaggaggag gagccggtcc ttgtacggaa
       61 ggacgaccat gtccttcagg ccgcgccccc ccgtctcgcg ataccacgcg agcgccagga
      121 gggcggccgg gttcctcccg acgtcgggga cccgggtcgc cgcgtcgcag gcgcgcgccc
      181 cctcgagcag gccgtcgacg tcgaagccct ggagcgccgc cgggaggagg ccgacggccg
      241 agagctgcga ggtccgcccg ccgacccagt cccacatggg gaagcggtcg agccagcgct
      301 gccgccgggc gagcttgtcg aggtcgctcc cgtcctgcgt cacggcgacg gcgtgccggc
      361 cgaagtcgag ccccttcgcc gtgtagacgg cctgcgcgac gagcatcccg ttgcgcgtct
      421 ccttcgtccc gcccgacttg gagacgacga cgacgagcgt cctcccgacc ccgccggccg
      481 cggcgatccg gtcgagctcc cgggccatgc cgtcggggtc ggtgttgtcg aagaagaacg
      541 ggcggagggg gtccgaggcg ccgccgagcg cgtcggcgac gagctgcggt ccgagcgccg
      601 acccgccgat cccgacgagg aggacgttct ggaacctccc gcccgactcg ggcgcgatct
      661 tcccggagtg gacgtctgcc gcgaacgcct tcacacgggc gaccatcgcc tcgatcgccc
      721 gcttcacctc ggggcccggg gcgagctcgg gggcgcggag ccagtagtgc ccgacccgtc
      781 gcccctcgtc gggattcgcg acggcccccg actcgagcgc ggccatcgcc gagaacgcgg
      841 acgggaacgc ctcgagcagg agcgggtcgc tcgtcttcgc gtgcgtggcg aggcacgtcc
      901 gcgagacgtc gaccaggagc gggacggcgg ggacgtgggc ggaaaggctc cggagacggg
      961 agagcgattc ggtcaacttt cacctcgggc cgggcgcgat gcgatcctcg tagactaacc
     1021 gcaaaggagc gcccgatggc cgacagccgg cagctcgtcc tcggaatcga cgtgggggga
     1081 acgaaggtcc tcgcgggagc ggtcgacgag ggcgggcgcg tcctcggtcg gggaaaggtg
     1141 aagagcccgt tccgcggcgg gccggaggag atgacggggg cgctcgtcgc cgcggccgac
     1201 gcggcgctcg cggaggcggg cgcccgccgg gcggacgtcg cggccctcgg cctcggcgtg
     1261 ccggggccgc tcgacgccga gcggacgacg ctcctccgcg cgatgaacct cggcgtggag
     1321 cggtggccgg tcgccgcctc gctcgagccg cacttccccg gggtcccgaa gcggctcgag
     1381 aacgacgttc gggccgcggc gctcggcgag gcgcgcttcg gcgcggcgaa gggcgagcgg
     1441 ctcgtcgtgg cgatctgggt cgggacggga atcggcggcg cggtcctcct cgacggcgac
     1501 ctgtggctcg gacgcaaccg gaatgccggc gagatcgggc agagcttcgt cgacctgaag
     1561 aaggccggga gcttcgacgg gaccctcgag gggatcgggg cgaaggtcgg gatgacggct
     1621 ttcctgcgcc gccggatcga gcgcggggag aagaccgtcg tggcgcgcgc cgtcctcgag
     1681 aaggacggcc gcctgaaggg ctccgaggtg aggaaggcgc tggccgcggg ggacgcgctc
     1741 gtcgagaggg cgctggcccg gtcggcccgc gccgtcggcg tggcgatcgg gacgctctgg
     1801 aacgtcttct cgcccgacct cttcgtcctc ggcggcggga tcgcggagga cgtcggcgag
     1861 ccgtacgtgg agcaggtccg ggcggcggcg ggccggtacg cgttcttcac ggacctcgcc
     1921 gaggtgcgcg tcgtccgatc ggcgctcggc gacgacgcgg ggatcctcgg cggcgcggtc
     1981 ctggcccgcg agggacaccg aagccggggc tgaggctcgc gaaccggcgc cgtccggccc
     2041 cggtttgacc ggacgcggcc ggatcgtgac aatcaaaggt tcgtgagctg cgccacctgc
     2101 tccatcggca ccctcgtgaa ccccggcggc aactacgtcg gggcccgcct cgccgacgcg
     2161 acggacggga aagtcacggt cgtcttcacc ggcgaggtcg ccgttcgccc gggcgagcgg
     2221 atcgtcgtcg agaccgagga ggggcccgag ttcgcggagg tgacgaccct ctcgcccgtc
     2281 ctggcgaagg cctgctccca gaagaaggcg aagcgcttcc tgcgcctcgc ttccgactcc
     2341 gagtcggccg atttcgccga gcggctcgag ctgcaggacg aggcggcccg gtgggcctcg
     2401 aagcggatcg ccgagctgcg cctgccggtg agcctcgtga agtgcgtcgt gccgttcgac
     2461 cggcgccgga tcacgctcct ctattcctcc gacgagcggg ccaacctccg cgacctctgc
     2521 cgggagatgt ccgatcgctt ccacctccgg gtcgagtcgc gaatggtcgg cgtgcgggac
     2581 gagacgaaga tcgtcggcgg gatcgggccg tgcggcctga cgctctgctg ctccacctgg
     2641 ctgaagggct tccacccggt cacgattcgg atggcgaagc tccaggggat caccccgaac
     2701 ccctcgaagc tgaccggcat gtgcggccgg ctgaagtgct gcctcgccta cgagctcgag
     2761 gggggcatcg aggcggcccg gaagatgggg agcaagggcc ccgaccggaa ggacgccgac
     2821 cgaaaggccc cgccggcccc gcccgtcccg ccggctccgg tcccgcccgc cgcctgacgg
     2881 cgtcgccgtc gggtagactt cgcgcacggc tcgattcccg aaacggcccc ctcccccgga
     2941 aggagcattc atggctgcag ttcgcaagcc gccgtcgccg gcctcgccgg cgggcgcccg
     3001 cgagcccgag agcgagctcg ggccgcgaaa gctgattcgc cccgccctgt cggacctccg
     3061 ccgcgacatg gggccgcgcg gcctccggaa aggggcgccg cccgagcaga cgaacgccga
     3121 gtcgttctac tacctcaagc agatgcaggc ccgcacgccg gtcgtcgtcc tcctggtcga
     3181 cggcgaggag ctgcgcggct ggatcgagtg gtacgacaag gacgcgatca agctgaaccg
     3241 ggagcacgcc ccgaacctcc ttctccagaa gcacgccatc aagtacctct acaaggagga
     3301 ggaggagagg ccgttccggc gccggaaggt ccggtcggaa cccgacggcg ccccgccgga
     3361 ggccggaggc cgctgaaggg ccgccgcggc gcagcacggg caccgcacgg gaagcggagc
     3421 tagactcgcc ccatgaagac cgagaagctc acgcgcgaag ccctgacagc gtatgccgcg
     3481 gagaatcggg aggctttcga ggcccttctg aaggacttcg tcgagatccc gagcgtctcc
     3541 gtggacccga cgaagaagag cgcgatccgc gagaacgcca agctcgcggc ggacgtgatc
     3601 cgccgcttcg gcggcgagtc gcgaatcctc gagaccgacg gccacccgat cgtcgccggc
     3661 gcctggggga agaacccgct ctggccgacc gtcacggtct acaaccacct cgacgtccag
     3721 cccgcctcga tcgagacgga gccgtggaag acggagccgt tcacgttcac gaagcagggc
     3781 gaccgctact tcggccgggg cacgaccgac gacaaggggc cggcgctgac ggcgctctac
     3841 ggcatcaagg ccgccctcga cgccgagctc ccggtgaacc tccgggtcct ctgggagttc
     3901 gaggaggaga tcggctcccc gagcttcgag aaggcgatcg ccgctcacgc cgacctgctc
     3961 gccaccgatt cggtcctcgt ctccgacacg atctggatcg cgcgcggcaa gccggcctgc
     4021 ccggccgggc tccgcggcct ccagggcttc ctcttcacgc tcgagacggg cgagacggac
     4081 cagcactcgg gcgtctgcgg cggcgcggcg cgcaacccga tggccgagct ga
//
LOCUS       DAOTEZ010000395         4125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_15943_length_4125_cov_4.804914, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000395 DAOTEZ010000000
VERSION     DAOTEZ010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15943_length_4125_cov_4.804914"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            368..1864
                     /locus_tag="P5164_16560"
     CDS             368..1864
                     /locus_tag="P5164_16560"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY45550.1"
                     /translation="MIRLHETVEVPRPIGEAFAYASDFANIEQWDPGVSGSVRLKDEP
                     VGPGSRFRLDVDFGPFTTPMVYVVTLFEAPHRVVLEGQGDRIRAVDDIRFEGIPGGTR
                     IDYTADLTFLGPSSLAEPFLRSALEGVGRRAVEGLKNALSREPKVPKASLVTTAIDHL
                     VLPGLLRFTRLGYRLRKGSFAPLAVSLRGRTAVVTGATSGLGRAAAAGLARLGARVVL
                     VGRDPRKAERAREEIRAATGNADLAVEVADLSLLSEVRALARRLLAKEKALHILVNNA
                     GILENERRTTPEGLEVSLVTNLLAPYVLTRRLRTRLLASAPARVVNVVSGGMYLAGLG
                     PAVFGGAEGAWDGTLAYARHKRALTVLTELWSDELFPHGVVVHAMHPGWAETPGVVRS
                     LPAFHRLTRPFLRNAEEGADTIVWLAAAPEVALSSGGLWLDRERHPAAVLPGTAGSAE
                     ERKRFVEELERMTGEPSRAPRASRTSPKARTGSRHASRRRAAASGVLA"
     gene            1861..2910
                     /locus_tag="P5164_16565"
     CDS             1861..2910
                     /locus_tag="P5164_16565"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRY45551.1"
                     /translation="MNPLPADAAVFESHLPQLLALVEKRLELQRGFHGRADLPALDPD
                     LFDLARELGLLLRVVYRYGLAEALEEESAWYASALSSRGPGRDALTLLLDSWIVAIHG
                     VVKPPECDRLARPLQEVRSRVDRILSRIATPPSTLLPSGVHELVDRLVRGEKARAQGL
                     LESRIAAGLAPHEAITDLLLPAMVEVGRRWEVGASGIWEEHLATETALGLLAGLAASA
                     RPREALPRLGLVGCVPNDEHQLAPAALSAYLELRGWNAPSLGRTLPAEQVVLAAEKLA
                     PHAVFLSMNLLARLHDAVGLVGALRRSRPGCPVFVGGRRAATARTWLEAAGARVVGSF
                     EEAHREATGELPDHA"
     gene            2903..4024
                     /locus_tag="P5164_16570"
     CDS             2903..4024
                     /locus_tag="P5164_16570"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY45552.1"
                     /translation="MRSLTAWRMVRHDRASTAGALLGVVSIVFLVGQQLSVLFGLLNY
                     MSVLVDHSGADAWVTSADPRNVDAANLVPGRIVDRVRGLREVEWAEPVLVGSGLFRTP
                     DGSFESVRVVGTRRPRLAGGPWSYAQADEGALLDLDAVTLDRLDLPKLGRPALGSGTE
                     IGDRRVRIGAITDGARGFQGTLVFASIDKVAEIAKTPPGRYSYVLVKLREGTDHATAL
                     ARLRLLLPEYSVFDGPELARRTRAYYFANTGIGGSFGFSTVIAVLIGAVIISLTMYTA
                     VLGRARDFAVLRAIGGRKRDVAVVVVAEALIIAGIGIFIGFVMLAALLNATGGSAIPS
                     FFPSRVAPILGAVTLAVSLLGSLVALRVALRAEPASVFH"
BASE COUNT          521 a         1552 c         1429 g          623 t
ORIGIN      
        1 ccggcctcct ctactggtac ggcatctacc cgctccacgt cctcgtcttc cggggaatgc
       61 tccgggagat cgcggcccgc gcgcggagaa acggccgacc cgaggccgcc cgggccggcc
      121 cggaaagcgg gatgccgcac gagccggaaa agcgctgagc cgagttggtg accacggcgt
      181 ggcaggtgta gccgggcagc tcgatgagtc tgcccggccg cgtcgagacg ttcggccagc
      241 acttcgcgga cgacgacgag acgccggacc gtcgaccagc cggggggggc gctagaatct
      301 ccgacgactg gtcgacgcgc tgcctgcttg cgtcactcgt cgcccgggtt ctcggaggga
      361 ggaccgcgtg atccggctgc acgaaaccgt cgaggtccct cgacccatcg gggaggcgtt
      421 cgcctatgcg agcgactttg cgaacatcga gcagtgggac ccgggcgtct cgggttcggt
      481 ccggctgaag gacgagccgg tcggtcctgg ctcgcgcttc cggctcgacg tcgacttcgg
      541 gccgttcacg acgcccatgg tgtacgtcgt gaccctcttc gaggcaccgc acagggtcgt
      601 actcgagggg cagggagacc gcatccgggc ggtggacgac atccgattcg aggggatacc
      661 cggcgggacc cggatcgact acaccgccga cctgaccttc ctcggaccgt cgtcgctcgc
      721 cgagccgttt ctgcgatccg cgctcgaagg cgtcggccgg agggctgtgg agggcctgaa
      781 gaacgccctc tctcgcgagc cgaaggtgcc gaaggcttcc ctcgtcacca cagccataga
      841 ccatctcgtc ctgccgggac tcctgcgctt cacgcggctc ggataccgct tgcggaaggg
      901 ctccttcgcc ccgctcgccg tctcgctccg ggggcgcaca gctgtcgtga cgggggcgac
      961 gtcggggctc ggccgggccg cagccgccgg cctcgcccgc ctcggggccc gcgtcgtcct
     1021 cgtcggacgg gacccgcgga aggcggagcg ggcgcgggag gagatccgcg ccgcgaccgg
     1081 gaacgccgac ctggccgtcg aggtggccga cctctcgctt ctctcggagg tgcgagccct
     1141 cgcacggcgc ctcctcgcga aggagaaggc cctccacatc ctcgtgaaca acgcgggcat
     1201 cctcgagaac gagcggcgga cgacgccgga aggcctcgag gtctccctcg tcacgaacct
     1261 cctcgcgcca tacgtcctga cgcgtcggct ccggacgcgg cttctcgcct cggccccggc
     1321 gcgcgtcgtg aacgtcgtct ccggcggcat gtatctcgcc ggcctcgggc ccgcggtctt
     1381 cggcggcgcc gaaggggcgt gggacggaac gctcgcctac gcgcgacaca agcgggcgct
     1441 gacggtgctg acagaactct ggtcggacga gctcttcccg cacggcgtcg tcgtccacgc
     1501 catgcaccca ggctgggccg agacgcccgg cgtggtgcgc tccctccccg cgttccaccg
     1561 cctcacgcgg ccgttcctga ggaacgccga ggagggggcg gacacgatcg tctggctcgc
     1621 cgccgccccg gaggtggcgc tctcgagcgg aggcctgtgg ctcgaccgcg aacggcaccc
     1681 ggcggctgtt ctcccgggca cggcggggtc agccgaggag cggaagcggt tcgtcgagga
     1741 gctcgagagg atgaccgggg agccctcccg cgcccctcgc gcctcccgga cgagcccgaa
     1801 ggcccggacc ggatcgcggc acgcatcgcg ccgtcgcgcc gcggcctccg gggtgctcgc
     1861 gtgaaccctc tcccggccga cgccgccgtc ttcgagagcc acctgccgca gcttctcgct
     1921 ctcgtcgaga agcgcctcga gctgcagcgg gggttccacg gaagggccga cctgccggcc
     1981 ctcgaccccg atctcttcga cctcgcacga gagctcggcc tccttctgcg cgtcgtctac
     2041 cgctacggcc tcgcagaggc gctggaggag gagtcggcct ggtacgcctc cgcgctctcc
     2101 tcccgtggcc cggggcggga cgccctgact ctccttctcg acagctggat cgtcgcgatc
     2161 cacggggtcg tcaagccccc cgagtgcgac cggctggccc ggccccttca ggaggtccgt
     2221 tcgcgcgtcg accggatcct ctcgagaatc gcgacccctc cctcgacgct cctcccttct
     2281 ggagtgcacg agctcgtgga ccggctcgtc cggggagaga aggcgcgcgc gcaggggctt
     2341 ctcgaatcgc ggatcgccgc aggcctcgcc cctcacgagg cgatcacgga cctcctgctc
     2401 cccgcgatgg tggaagtcgg ccgccgctgg gaggtcggcg cgtccgggat ctgggaggag
     2461 cacctcgcca cggagacggc tctcgggctc ctcgccgggc tcgccgcttc cgcccggccg
     2521 agggaagcgc tcccgcgcct cggcctcgtc ggctgcgtac cgaacgacga gcatcagctc
     2581 gccccggcgg ccctgagcgc ctacctcgag ctgcgtgggt ggaacgctcc ctcgctcggc
     2641 cgcacgctcc cggcagagca agtcgtcctc gcggcggaga agctcgcgcc ccacgccgtc
     2701 tttctctcca tgaacctcct cgcccggctg cacgacgccg tcgggctcgt cggagcgctc
     2761 cggcgctcgc ggccgggctg ccccgtcttc gtgggcggac gcagggccgc gacggcgcgg
     2821 acctggctcg aggcggccgg ggcgcgcgtc gtcggcagct tcgaggaggc ccatcgcgag
     2881 gcgacggggg agctgccgga ccatgcgtag tctcaccgcc tggcggatgg tccgccacga
     2941 ccgcgcctcc accgcgggcg ccctcctcgg ggtcgtctcg atcgtcttcc tcgtggggca
     3001 gcagctgtcg gtccttttcg gcctcctgaa ctacatgtcg gtcctcgtcg accacagcgg
     3061 cgcggacgcc tgggtgacga gcgccgaccc gaggaacgtg gacgccgcca acctcgtccc
     3121 gggtcggatc gtcgaccgcg ttcgggggct gagggaggtg gagtgggccg aacctgtcct
     3181 cgtcggaagc ggccttttcc ggacgccgga cggctcgttc gagtcggtcc gggtcgtcgg
     3241 gacccgccgc ccccggctcg cagggggacc gtggagctac gcgcaggccg acgagggagc
     3301 gctcctcgac ctcgacgccg tcacactcga ccggctcgac ctcccgaagc tcggccggcc
     3361 cgccctcggt tccgggacgg agatcgggga ccgccgcgtc cgcatcgggg cgatcaccga
     3421 cggggcccgc ggattccagg ggaccctcgt cttcgcctcg atcgacaagg tcgccgagat
     3481 cgcgaagacg ccccccgggc gctactccta cgtcctcgtg aagctccgcg aagggaccga
     3541 ccacgcgacg gctctcgccc ggctgcggct cctcctgccg gagtactcgg tcttcgacgg
     3601 ccccgagctg gcccggagga cccgggccta ctacttcgcg aacacgggga tcggcggcag
     3661 cttcggcttc tcgaccgtca ttgccgtcct catcggcgcc gtcatcatct cgctcacgat
     3721 gtacacagcc gtcctcggcc gggcccggga ttttgccgtc ctccgggcta tcggcggccg
     3781 caagcgcgac gtcgccgtcg tcgtcgtcgc cgaggccctc atcatcgcgg ggatcggcat
     3841 cttcatcggc ttcgtcatgc tcgccgcgct cctcaacgcg acggggggct cggcgattcc
     3901 gagcttcttc ccctcgcggg tcgcaccgat cctcggtgcc gtcacgctcg ccgtcagcct
     3961 cctcggttcg ctcgtcgcac tgcgggtcgc gctccgggcc gagcccgcct cggtgttcca
     4021 ctgaaaccgt acgagcccgg ccccgccccg gctcgccgtg cggcccgact ttcgtcttcg
     4081 cggaggccga cgtggcgacg ctgacgctcg aggcgatcgc gcgcg
//
LOCUS       DAOTEZ010000396         4114 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_16010_length_4114_cov_5.652624, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000396 DAOTEZ010000000
VERSION     DAOTEZ010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16010_length_4114_cov_5.652624"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2167)
                     /locus_tag="P5164_16575"
     CDS             complement(<1..2167)
                     /locus_tag="P5164_16575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRY45553.1"
                     /translation="MTPSRRVPASPEPAADISCANAVLPVAGMTCASCARLIEWKVGR
                     LEGVRSVRVSVVTDSLHVDFDASRLDVARIAACVRRAGFGTPGFGADDSDGSAGLETG
                     GAARTRLVLGLVLTGPLIVYSMGRDLGLPAFPHDQLLLLLAATIVQVVVGVPFYVRSW
                     RSLRSGSSSMDVLVALGSTVAWGASAGVTLGLLPGSGVYFETGAAIVTFVTLGRFLEA
                     RARGRASRALQGLLDLRPLRARVVREGVEVEVPSEEVRAGETVVVRPGERVPVDGLVR
                     EGRTACDESMVTGESMPVSKGPGDEVIGATVNRDGLVRVEATRVGADSTLSRIVRLVE
                     EAQARKAPVEKVADAVGRVFVPLVLVAALATLVLWLFVARIDVPGALMNAVAVLIIAC
                     PCAIGLATPMAVLVGTTRGASRGILFRTGEALERVGAATVVVFDKTGTLTRGEPGLTD
                     VVASRPGEEPEVLRLAASAELGSEHPVGRALARAGAARGLALSSPGELRAVAGAGIRA
                     RVEGREVLVGSLWLMREAGVSLEALEPEIDRLQAEGKTAMVVAAAPPDGGAPQAVGVL
                     AVADAVKPEARQAVAELSRLGLDVVMLTGDNLRTAEAIGREVGIERVRAEVRPEEKAE
                     AIRNLREAPRPPGTPPPVVVMVGDGINDAPALAQADVGIALGTGTDVAMEAAAVTLMS
                     GDLRGVGRAIALSRASLQTVHQNLVWALFYNVALVPVAAY"
     gene            complement(2190..2633)
                     /locus_tag="P5164_16580"
     CDS             complement(2190..2633)
                     /locus_tag="P5164_16580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY45554.1"
                     /translation="MTERPGAARRGTPFERLLLVDDEEPLVELLTLSLGELGYQVSGF
                     TDARAALDAFLEAPEAFDALISDISMPHLPGLELAGRILRVRSGMPVVLTSGWVREED
                     EAAGRSLGIIGFAAKGSSVEEIVHVIDRLLRGEVGPGDPSGAEPS"
     gene            complement(2846..>4114)
                     /locus_tag="P5164_16585"
     CDS             complement(2846..>4114)
                     /locus_tag="P5164_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY45555.1"
                     /translation="LSCSVAMFGNYYVFDALYPVTPLLEKAFGFTGAQVGLLDTAYNV
                     AALLTLIAGGILIDRMGVAASAVLFGAIGAAGSVAIAVLPALLPDAPWVGMMLGRFLL
                     GVGSECFIVAATTVVGRWFKGKEISFALAIQLLVGRLGSWAADKSPDLAKSLFETWQP
                     PLLLAAVLGVVWLVFAIVYAGLEKRAARVYAVKKAGATDKLVWKDLVRFDLGYWWVVG
                     LCVAFYSTIFPFRTFANLYFIEAKGLLPEDAGNLKSILPLLSMIGMPLFGLLVDKIGK
                     RALLMAAGSALLVPPFFLLAFSSAPPTLLMAMLGLAFALVPAVLWPAVTYLVPEARLG
                     SAYALMTFCQQVGWAGMSWGLGLLKDASGASAAHPAGWAPVMVALGALASVGFVFSFL
                     LWRSERGAKGHGLDAVTPTTAEEAKPVEPA"
BASE COUNT          653 a         1539 c         1463 g          459 t
ORIGIN      
        1 cgtaggcggc gaccgggacg agcgcgacgt tgtagaagag cgcccagacg aggttctggt
       61 ggacggtttg cagcgacgcc cgcgagaggg cgatcgcccg tcccacgcct cggaggtcgc
      121 cgctcatcag ggtgacggcg gccgcctcca tcgcgacgtc cgtcccggtc ccgagggcga
      181 tgccgacgtc ggcctgcgcc agggcgggcg cgtcgttgat cccgtcgccc accatcacga
      241 cgacgggcgg cggcgtgccg ggcggccgcg gggcctcccg caggttgcgg atcgcctccg
      301 ccttctcctc cgggcggacc tccgcgcgca ccctctcgat cccgacctcg cggccgatcg
      361 cctcggcggt ccgcaggttg tcccccgtca gcatgacgac gtcgagcccc aggcgcgaga
      421 gctccgcgac ggcctgacgg gcctccggct tcacggcgtc ggcgacggcc aggacgccga
      481 cggcctgcgg cgctccgccg tccggcggcg ccgcggccac gaccattgcg gtcttgccct
      541 ccgcctggag ccggtcgatc tcgggctcga gcgcctcgag ggacaccccc gcctcgcgca
      601 tgagccagag cgagccgacg aggacctcgc gcccttcgac gcgcgcgcgg atccccgcgc
      661 cggccaccgc ccgcagctcc ccgggcgacg acagggcgag cccgcgggcc gccccggccc
      721 tcgccagtgc ccggccgacg gggtgctccg agcccagctc ggcgctcgcc gcgaggcgca
      781 gcacctcggg ctcctccccc ggtctcgagg cgacgacgtc ggtcaggccc ggctcgccgc
      841 gggtgagcgt gcccgtcttg tcgaagacga cgaccgtcgc cgcgccgacg cgctcgagag
      901 cctcgccggt ccggaagagg attccgcgcg aggcgcctcg cgtcgtcccg acgaggacgg
      961 ccatcggcgt agcgaggccg atcgcgcacg ggcaggcgat gatgaggacg gccaccgcgt
     1021 tcatcagcgc cccgggcacg tcgatccgcg cgacgaagag ccagagaacg agcgtggcga
     1081 gcgcggcgac gaggaccagg gggacgaaga cgcgtccgac ggcgtcggcg accttctcga
     1141 ccggggcttt ccgagcctgg gcttcctcca cgaggcggac gattcgggaa agcgtggagt
     1201 cggccccgac ccgcgtcgcc tcgacccgca cgagaccgtc gcggttgacg gtcgcgccga
     1261 tcacctcgtc gcccggcccc ttggagacgg gcatcgactc gccggtgacc atcgactcgt
     1321 cgcaggcggt ccgtccttca cggaccaggc cgtcgaccgg gacccgctcg ccgggccgga
     1381 ccacgaccgt ctccccggca cggacctcct cagaaggcac ctcgacctcg actccttcgc
     1441 ggacgacgcg ggcccgcagg ggccgcaggt cgaggagccc ctgaagcgcc cgcgaggccc
     1501 ggccccgggc gcgggcttcc aggaaccgcc cgagcgtcac gaaggtgacg atcgcggccc
     1561 ccgtctcgaa atagacgccg gagcccggga ggagcccgag cgtgaccccc gcgctcgcgc
     1621 cccaggcgac ggtggagccg agcgccacga ggacgtccat gctcgacgag ccggagcgga
     1681 ggctccgcca ggaacggacg tagaacggca ccccgacgac gacctggacg atcgtcgcgg
     1741 ccagcagcag aaggagctgg tcgtggggga aggccggcag gccgaggtcc cggcccatgc
     1801 tgtagacgat cagcgggccg gtgaggacga gtccgaggac gagccgggtc ctcgcggccc
     1861 ccccggtctc gagccccgcg gagccgtcgg agtcgtcggc tccgaagccg ggcgtgccga
     1921 agcccgcccg cctcacgcag gcggcgatcc gggcgacgtc gaggcgggag gcgtcgaagt
     1981 caacgtgcag cgaatcggtc accacgctga cgcgcacgga gcgcacccct tcgagccgtc
     2041 ccaccttcca ctcgatcagc cgggcgcagc tcgcacaggt catccccgcc accgggagaa
     2101 cggcgttcgc gcaggagatg tcggcggcgg gctccggtga ggccgggacg cggcgggacg
     2161 gcgtcatcgc actcctcccc cgctgggcgt caggacggct cggcgcccga ggggtcgccc
     2221 gggcccacct cgccgcgcag gagccggtcg atcacgtgaa cgatctcctc gaccgaggag
     2281 cccttggccg cgaacccgat gatgcccagg gaccgaccgg ccgcctcgtc ttcctcccgg
     2341 acccagccgg aggtcaggac gaccggcatt ccggagcgca cgcggaggat tcgacccgcg
     2401 agctccagcc ccggcaggtg aggcatcgag atgtcggaga tcagggcgtc gaacgcctcc
     2461 ggcgcctcga ggaaggcatc gagagccgct cgcgcgtccg tgaagcccga cacctggtac
     2521 cccagctcgc cgagggagag cgtcaggagc tcgacgagcg gctcttcatc gtcgacgaga
     2581 aggagccgct cgaacggcgt cccccggcgc gcggcccccg gcctttccgt cataaccggc
     2641 cttcccgccg gctgcatacg gccaagctca ccggcacctc gcggccggaa ccgccgtccc
     2701 tgctcatcac accgacattc tctctccctc gacccggaat gtctcgcgtg acgggatcga
     2761 ggcgacgcag tctcgacgga gcgcagagcc gcccgcccgc gaacgcccgt cgaccggggc
     2821 gaagcgccgg cactcccggg gccgatcacg ccggctcgac gggcttcgcc tcctccgccg
     2881 tcgtcggcgt caccgcatcc aggccgtgcc ccttcgcccc ccgctcgctc ctccagagga
     2941 ggaacgagaa gacgaagccg accgaggcga gagcgccgag cgcgaccatc acgggcgccc
     3001 agccggcggg gtgcgcggcg ctcgcacccg aagcgtcctt caggaggccg aggccccagc
     3061 tcatccccgc ccagccgacc tgctggcaga acgtcatgag cgcgtaggcc gagccgaggc
     3121 gcgcctcggg aacgaggtag gtcaccgccg gccagaggac cgccgggacg agcgcgaacg
     3181 ccagcccgag catcgccatc aggagcgtcg gcggggccga ggagaacgcg agcaggaaga
     3241 acggcgggac gaggagcgcc gatccggccg ccatcaggag cgccctcttc ccgatcttgt
     3301 cgacgagcag gccgaagagc ggcatcccga tcatcgagag gagcgggagg atcgacttca
     3361 ggttcccggc gtcctcgggc aggagcccct tcgcctcgat gaagtagagg ttcgcgaagg
     3421 tccggaacgg gaagatcgtc gagtagaacg cgacgcagag gccgacgacc caccagtagc
     3481 cgaggtcgaa ccggaccagg tccttccaga cgagcttgtc ggtcgcgccg gccttcttca
     3541 ccgcgtagac gcgggccgcc cgcttctcga ggccggcgta gacgatcgcg aagacgagcc
     3601 agacgacgcc gaggacggcc gcgaggagga ggggcggctg ccaggtctcg aagagcgact
     3661 tcgcgaggtc gggcgacttg tcggcggccc acgagccgag ccgcccgacg agaagctgga
     3721 tcgcgagggc gaaggagatc tccttcccct tgaaccagcg gccgacgacc gtcgtcgccg
     3781 cgacgatgaa gcactccgag ccgaccccga ggaggaaccg gcccagcatc atccccaccc
     3841 acggcgcgtc cgggaggagc gcggggagta cggcgatcgc gacgctcccg gccgcgccga
     3901 tcgccccgaa gagaacggcc gaggccgcga cgcccatccg gtcgatcagg atcccgcccg
     3961 cgatcagcgt caggagcgcc gcgacgttgt aggccgtgtc gaggagcccg acctgcgctc
     4021 ccgtgaaccc gaacgccttc tcgaggagcg gcgtgaccgg gtagagggcg tcgaagacgt
     4081 agtagttgcc gaacatcgcc acgctgcacg agag
//
LOCUS       DAOTEZ010000397         4091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_16129_length_4091_cov_5.407086, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000397 DAOTEZ010000000
VERSION     DAOTEZ010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16129_length_4091_cov_5.407086"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="P5164_16590"
     CDS             complement(<1..363)
                     /locus_tag="P5164_16590"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HRY45556.1"
                     /translation="MTANPLPSAEREALVAAATAARENAAAPYSNFHVGAALLAGDGR
                     IFGGCNVESASYGLTCCAERTAVFKAISEGVRSFRAVAVVTGADEPTSPCGACRQVLW
                     DQCRDIAVVMATTSGKLEE"
     gene            complement(483..1301)
                     /locus_tag="P5164_16595"
                     /pseudo
     CDS             complement(483..1301)
                     /locus_tag="P5164_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008879723.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
     gene            complement(1318..2604)
                     /locus_tag="P5164_16600"
     CDS             complement(1318..2604)
                     /locus_tag="P5164_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HRY45557.1"
                     /translation="MDRFTGFIGLAVILLTCFALSRNRKAIRWATIFWGVVLQFALGW
                     LVISWKAGANALKSFSDGITGAIAYSDEGAKFVFGWLADPAIGGAISAKTGIDFSPFL
                     FAFKVMPIVIFIASFFTILYYLGVMQRIVQVFAVVMTKLLRISGAESLAVSANIFIGQ
                     TEAPIIIAPYIPKMTNSELLTMMTGGMAHISGAVLLAYAAMGIPASYLITASVMAAPA
                     TIVIAKLLRPEVDTPETSGAVKVKIEHEHANVIEAAAGGASQGVSLAINIVGMLIAFI
                     ALVSLFNGLLGWVGTFIGFATPGPGVDPATTLSVQWLFSKLLWPLAWVMGVPAADCSK
                     VARLIGFKTVINEFVAYAEMTKIPLSEWTEKGRLIASFALCGFANFSSIGIQIGGIGG
                     LAPSRKSDIARLGLWALLGGSLATFMSATIAGILLG"
     gene            complement(2864..3583)
                     /locus_tag="P5164_16605"
     CDS             complement(2864..3583)
                     /locus_tag="P5164_16605"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY45558.1"
                     /translation="MSKDKISRKELKENELEHALVEARDYVVSHEATAKRWTAIVVGA
                     VVLVGLVWGGFALRSRRLDARLSTALATFDAALATEGSGTLPGVTVYKDAAERLEAAK
                     SLLRSLADDAPGSRAGQAARTMLLALEGGATPPARLVDGARDLAKRSPGSIASGVAAL
                     AGLEADAAGGRVKEAISSAKGYLEAAKPPLPKDVLLFTLARLYEKDGQAAEARSSYQR
                     VVADFPDSPLRFEAQQKAQSL"
     gene            complement(3608..3928)
                     /locus_tag="P5164_16610"
     CDS             complement(3608..3928)
                     /locus_tag="P5164_16610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45559.1"
                     /translation="MEKAPEETFRVTDRALAALRAAGLDPARTFVVVQHVLGCGGAGY
                     RLVFRETPLDGGVVLDGPEGLRISLDAYSRRRLEGAALDYDLEKETEGFRLDHPDAAF
                     ASFC"
BASE COUNT          619 a         1410 c         1495 g          567 t
ORIGIN      
        1 ctcctccagc ttcccgctcg tcgtcgccat cacgacggcg atgtcgcggc actggtccca
       61 gaggacctgg cggcaggcgc cgcacggaga ggtcggctcg tcggcgcccg tcacgacggc
      121 gacggcccgg aacgagcgga cgccttcgga gatcgccttg aagaccgccg tcctctccgc
      181 gcagcaggtg aggccgtagc tcgcagactc gacgttgcag cctccgaaga tccggccgtc
      241 cccggccagg agcgccgctc cgacgtggaa gttcgagtag ggggcggccg cgttctcccg
      301 cgccgccgtg gccgccgcga cgagggcctc gcgctcggcc gaggggagag gattcgccgt
      361 catcgcgccg ctcgtccccg ccgcaccgcg ggcttcgcgc cccggcgcgg ccccgaggtc
      421 gcctttcgtg ccgggcgctt cgcgcgccgc gccggctcga cgacgggccg gtcggacgag
      481 cgtcccttcc ccatcgcctc gacgagggcc gagaggagcc gcacgaagcg gtccttcacg
      541 cgcctcgtcg tctcgaggac ctcgtcgtga cggagcggct gcttcaggac gccggccgcc
      601 atgttcgtga tgcaggagac gcccaggacg ccgacgccca tgtgggagag ggcgatgacc
      661 tcggggacgg tcgacatgcc gacggcgctc gcgccgagcg tccggaacgc gcggatctcg
      721 gccggcgtct cgtaggacgg cccggtgacg ccgacgtaga ccccctcccg gagggggaca
      781 cggatcttcc gggccacggc cttcgcgagc acccgcaggt cggcgcggta cgcgtcggac
      841 atgtcgggga atcgagcgcc gagcgaatcc tcgttcgggc cgatcagggg gttcgtcccg
      901 aagaggttga cgtggtccga gatgagcatc aggtcgccgg cggagaagcg cgtgtcgatc
      961 ccccccgccg cgttcgtcac gatgacgtcg cggcagccga ggaggccgag gacgcgggcc
     1021 ggggtgacga cgagcgacat cggatgcccc tcgtagtagt ggatacggcc cgcgaggacg
     1081 acgatcggga ctccggcgag cgtcccggcg acgagcgtcc ccgcgtggcc cacgaccgca
     1141 gaggccggga agcccgggat ctgcgcgtac gggatctcga cccggtcctc gaccgccgcg
     1201 gcgaagtcgc ccaggcccga gcccaggatg acgccgacga cgggcgggac ggggatccgc
     1261 gcccggagga actcgacggt ttcggcgaag cgagggtcca tctctcgtct ccttccgtca
     1321 gccgaggagg attccggcga tcgtcgcgct catgaaggtc gcgagcgagc cgccgaggag
     1381 cgcccagagc ccgagccggg cgatgtcgct cttgcgcgac ggcgcgaggc cgccgatgcc
     1441 gccgatctgg attccgatcg aggagaagtt cgcgaagccg cagagggcga aggacgcgat
     1501 caggcgtccc ttctccgtcc actccgagag cgggatcttc gtcatctcgg cgtaggccac
     1561 gaactcgttg atgacggtct tgaagccgat caggcgggcg accttcgagc agtcggccgc
     1621 ggggacgccc atgacccagg cgagcggcca gaggagcttc gagaagagcc actggaccga
     1681 gagcgtcgtg gccgggtcga cgcccgggcc gggcgtcgcg aagccgatga aggtgccgac
     1741 ccatccgagg aggccgttga agagcgacac gagcgcgatg aaggcgatca gcatcccgac
     1801 gatgttgatc gcgagcgaga cgccctggga ggcgccgccc gcggccgcct cgatgacgtt
     1861 cgcgtgctcg tgctcgatct tcaccttcac ggctcccgaa gtctccggcg tgtcgacctc
     1921 cggccgcagc agcttcgcga tgacgatcgt cgccggcgcg gccatgacgg acgccgtgat
     1981 gaggtagctc gccgggatcc ccatcgccgc gtacgcgagc aggaccgccc ccgagatgtg
     2041 ggccatcccg cccgtcatca tcgtcaggag ctcggagttc gtcatcttcg ggatgtacgg
     2101 cgcgatgatg atcggcgctt ccgtctgccc gatgaagatg ttcgccgaga ccgcgaggga
     2161 ctccgccccc gagatgcgga ggagcttcgt catcacgacc gcgaagacct gcacgatccg
     2221 ctgcatcacg ccgaggtagt agaggatcgt gaagaacgac gcgatgaaga tgacgatcgg
     2281 catcaccttg aaggcgaaga ggaacggcga gaagtcgatc ccggtcttcg ccgagatcgc
     2341 gccgccgatc gccgggtcgg cgagccaccc gaagacgaac ttcgcccctt cgtccgagta
     2401 ggcgatcgcg cctgtgatcc cgtcggagaa cgacttcagc gcgttggcgc ccgccttcca
     2461 ggagatgacg agccagccga gcgcgaactg gaggacgacg ccccagaaga tcgtcgccca
     2521 gcggatggcc ttcctgttcc gcgagagcgc gaagcaggtg aggaggatga cggcgagtcc
     2581 gatgaaaccg gtgaaacggt ccaaggaggc ctcctgaggg ctctttcaga gctcgtgggg
     2641 ttctttgccg cgaccccgcg cccgccggcg gctcaccccg cggccttcgc ggctccgggg
     2701 acggtccgga gcgcggagac ggcgacgggc gcctggcggc ggatggatgt cgcgatccgg
     2761 gaaggatacc ccgagaccgg ggctcccggc cgctccgaaa cgtcgcttcg aggatccccg
     2821 gaacggaccg gggcggccga gagccgcccc ggtcccgaga ggatcagagc gactgggcct
     2881 tctgctgggc ctcgaaacgg agcggggagt cggggaaatc cgcgacgacg cgctggtagc
     2941 tcgatcgcgc ctcggcggcc tgtccgtcct tctcgtacag acgcgccagc gtgaagagga
     3001 ggacgtcctt cggcagcggg ggcttcgccg cctcgaggta gcccttcgcg gaggagatcg
     3061 cctccttgac ccgccctccg gccgcgtcgg cctcgaggcc ggcgagcgcg gcgacgcccg
     3121 aggcgatgga gcccggggac cgcttcgcga ggtcgcgcgc tccgtcgacg agccgcgccg
     3181 gcggcgtggc gcctccttcg agggcgagga gcatcgtgcg cgccgcctgg ccggcacgcg
     3241 agcccggggc gtcgtccgca agcgagcgga gcagcgactt cgcagcctcg agccgttcgg
     3301 cggcgtcctt gtagaccgtg acgccgggga gcgtcccgga gccttcggtc gcgagcgccg
     3361 cgtcgaacgt cgccagcgcc gtcgagagac gggcgtccag cctccgggag cggagcgcga
     3421 agccgcccca gacgagcccg acgaggacga cggcgccgac gacgatcgcc gtccacctct
     3481 tcgccgtcgc ctcgtggctc acgacgtagt cgcgcgcctc gacgagagcg tgctcgagct
     3541 cgttttcctt cagttccttc cgggagatct tgtccttgga catgcgtgcg gtcggggccg
     3601 ggccggatca gcagaacgag gcgaacgccg cgtccggatg gtcgagccgg aagccctcgg
     3661 tctccttttc gaggtcgtaa tcgagcgccg ccccttcgag gcggcgccgg gagtatgcgt
     3721 cgagagagat tcggagtccc tccggcccgt cgagaacgac tccgccgtcg agcggcgtct
     3781 cccggaagac gagccggtag ccggcccccc cgcagccgag gacgtgctgg acgacgacga
     3841 acgtccgggc agggtccagg cccgcggcgc gcagcgccgc gagggcccgg tcggtgaccc
     3901 ggaacgtctc ctcgggggcc ttctccatct agcggcgggc ggccttcctc cggggcgcct
     3961 ttttcggcgc gggcttccgg acgggtgcgg ccttcttcgg cgccggcttg cgggcgggct
     4021 tgcgggcggg cttgcgagga gaagccttct tgcgggaagc ccacttcggg gccgccttcg
     4081 ccggcgtcac g
//
LOCUS       DAOTEZ010000398         4082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_16186_length_4082_cov_4.106531, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000398 DAOTEZ010000000
VERSION     DAOTEZ010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16186_length_4082_cov_4.106531"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..894)
                     /locus_tag="P5164_16615"
     CDS             complement(109..894)
                     /locus_tag="P5164_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY45560.1"
                     /translation="MKRLAVANQKGGVGKTTTAISLAAALSVLEKRVLLVDFDPQGNT
                     TGGLGVEKGGLERTVYDWILGNASLGEVARPTELAGLKLIPASRDLVGAEIELVGVEE
                     REFLLARKLDGLAEAFDYVFIDCPPSLGLLTLNALTAADGVLVPIQCEYYALEGVSEL
                     VSTIERVREGLNPSLAIAGVVATMLDERLNLSRQVLEEIRRYFGATFLEPPVPRNVRL
                     GEAPSFGKPIFLYDIRSRGAEAYLAIARELLRRDLAGGNGGQP"
     gene            complement(1022..1672)
                     /locus_tag="P5164_16620"
     CDS             complement(1022..1672)
                     /locus_tag="P5164_16620"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070043 - rRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRY45561.1"
                     /translation="MTGSAATVLAVLEGAGFPIGVAAAGRIAAYLDLLLRRNEVVNLV
                     SRKEATVETLLTRHVLDALEGLRLLPVPAGRPLRLLDIGSGGGFPAIPLLLARGDLEG
                     VLVESTGKKARFLEEAIGALELTARVVNARFPDPAIDRMKSAPPCDLLTSRAVADAGG
                     ILRQARPVMGPGAVALFWTTEPLVGELRRDLPRAVVSFSRSAGAERRGIARVECFT"
     gene            complement(1669..3567)
                     /gene="mnmG"
                     /locus_tag="P5164_16625"
     CDS             complement(1669..3567)
                     /gene="mnmG"
                     /locus_tag="P5164_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRY45562.1"
                     /translation="MRERYEVVVVGGGHAGAEAAAAASRLGKRTLLLTRERAAIGRMS
                     CNPAIGGLAKGQVVREIDALGGVMARVADRTGIQFKVLNASRGPAVQGLRCQSDRRAY
                     ASAVVDHLSRQPNLTIAEGTAAGFLVRGGRLAGLRLEDGAEILCLSAVVTSGTFLRGL
                     VHVGERREEAGRWGEAPCSALSEGLRALGLRTGRMKTGTPPRVHGKSIDRTKMLRSAG
                     DERPRRFSLRSRSEPFPSLPQVDCWLSHTNERTHDLIRTNLGRSPLYSGRIEGRGPRY
                     CPSIEDKVVRFPERERHTVFVEPEGHGTEWTYLGGLSMSLPADLQVEIVRSLPGLERA
                     EILRPAYAVEYDVVYPEQLEDTLETRALPGLFLAGQVNGTSGYEEAAGQGLIAGANAA
                     AVAEGRIGPPFTLGRHEAYIGVMIDDLVTLGADEPYRLLTSRAEHRLLLGADSAYARL
                     VPRAVSAGLLSEEEAEPLLEREARQAVAREALGGLRVVPDRETVAALGRLAVSLAEES
                     TGAGLLRRPDSPGELRGWLEERLPGPLGGLDEEEWSRLVNDLRYEGFLRREREALERV
                     RRSEEKKIPAGFRYAGIPGLSAEAAEKLERHRPRTLGQAARIPGVTAAAVTLLLARLA
                     IRGGEAAA"
     gene            complement(3564..>4082)
                     /locus_tag="P5164_16630"
     CDS             complement(3564..>4082)
                     /locus_tag="P5164_16630"
                     /inference="COORDINATES: protein motif:HMM:NF024045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45563.1"
                     /translation="RGERVRLIDTAGLRQAEELVERLGVETARRAAASADLLLLVRAP
                     DASPGEQERLPEGVPVLLVATKADLGAACEEGALPVSARTGRGLETLREEISRRLGAG
                     PEAGVWGPLPRQGEALRRAAEALRPLGEELPAELAAAGVRAALHALGEITGETATEEL
                     LERIFERFCVGK"
BASE COUNT          573 a         1542 c         1402 g          565 t
ORIGIN      
        1 ggtcgaggtc gatcatctgg accgagctcc ccgggcgggg ctcggccggg gccggccggg
       61 tggagggaat gagggccgag aggcccttcc cgagcgcggg tttcttggtc acggctgccc
      121 tccgttccct ccggccaggt cgcgccgcag gagctcgcgg gcgatcgcga ggtaggcctc
      181 ggccccgcgc gagcggatgt cgtaaaggaa gatcggcttg ccgaacgagg gagcctcgcc
      241 gagccttacg ttccgcggga ccggcggctc caggaaggtc gccccgaagt agcgacggat
      301 ctcttcgagg acctggcggg agaggttcag ccgctcgtcc agcatcgtcg cgacgacgcc
      361 ggcgatcgcg aggctcgggt tgagcccctc ccggacccgc tcgatcgtcg agacgagctc
      421 ggagacgcct tcgagggcgt agtactcgca ctggatcggc accaggacgc cgtcggccgc
      481 ggtcaaggcg ttgagtgtca ggagaccgag agagggcggg cagtcgataa agacgtagtc
      541 gaacgcttcg gcgaggccgt ccagcttccg ggcgaggagg aactcccgct cctcgacgcc
      601 gacgagctcg atctccgcgc cgacgaggtc gcgcgaggcc gggatgagct tcagcccggc
      661 gagctccgtc gggcgggcca cctcgcccag ggaggcgttc ccgaggatcc agtcgtagac
      721 cgtcctctcg aggcccccct tttccacgcc gagcccgccc gtcgtgttcc cctgcgggtc
      781 gaagtcgacg agaagcaccc gcttctcgag gaccgagagc gccgcggcga ggctgatcgc
      841 cgtcgtcgtc ttaccgacgc cccccttctg gttcgcgacc gcgagccgtt tcacgtggaa
      901 ctcccccttc gccggtccct gcggccggcc tttccgcggc tctgtccggc gcccggatcg
      961 cgcggcttcg tgacgacgac ggggcggatc ggggcgctgt ggatctccct gtggaaatgt
     1021 ttcacgtgaa acactctacc cgggcgatcc cgcgccgctc cgcgccggcg ctcctggaga
     1081 acgaaaccac ggccctcggc aggtcgcggc gaagctcccc gacgagcggc tccgtcgtcc
     1141 agaaaagtgc gacggctccc ggccccatca cgggacgggc ctggcgaagg atccctccgg
     1201 cgtcggcgac ggcgcggctc gtcaacaggt cgcagggggg ggcgctcttc atccgatcga
     1261 tcgcgggatc ggggaatcta gcgttcacga cacgggccgt caattcgagg gccccgatcg
     1321 cctcctccag gaaccgcgcc ttcttccccg tcgactcgac gaggacaccc tccaggtctc
     1381 cgcgcgcgag gagcagggga atcgccggga agccccctcc cgacccgatg tccaggagcc
     1441 gcaggggccg cccggccggg accggtagaa gtcgcaggcc ttccagcgcg tcgagaacgt
     1501 gccgtgtgag gagcgtttcg accgtcgctt ccttccgcga gaccaggttg acgacctcgt
     1561 tccgccgaag gagcaggtcg aggtaggcgg cgatcctccc ggccgcggcg acgccgatcg
     1621 gaaaccccgc cccctcgagc accgcgagga ccgtcgccgc cgaccccgtc acgcggccgc
     1681 ctcgccaccc cggatcgcga ggcgggcaag gagaagcgtg accgccgcgg cggtcacgcc
     1741 cggaatccgc gccgcctgtc cgagcgttct gggtcggtgc cgctccagct tctcggccgc
     1801 ctcggcggag aggcccggga tcccggcata ccgaaacccg gccgggatct tcttctcctc
     1861 ggagcggcgg acccgctcga gcgcctcccg ctcgcgacgc agaaaacctt cgtaccggag
     1921 gtcgttcacg agcctcgacc actcctcctc gtcgagccca ccgagcggac ccgggagtcg
     1981 ctcttcgagc cagccccgga gctcgcccgg ggagtccggc cgccggagga gccccgcgcc
     2041 ggtcgattcc tcggcgagag agacggcgag ccgtccgagc gcggcgacgg tctcccgatc
     2101 gggaacgacc cgcaggccgc cgagcgcctc gcgcgccacc gcctgccggg cctcccgctc
     2161 gaggagcggc tccgcctcct cctccgagag gaggccggcg gagaccgccc tcgggacgag
     2221 ccgggcgtac gccgagtcgg cgccgaggag gagccgatgc tccgcccggg aggtgaggag
     2281 gcgatagggt tcgtccgcgc cgagggtcac gaggtcgtcg atcatcacac cgatgtaggc
     2341 ctcgtgccga cccagcgtga acgggggccc gatccggccc tccgcgacgg cggccgcgtt
     2401 cgccccggcg atcaacccct ggcccgccgc ctcctcgtag ccggaggtcc cgttcacctg
     2461 gcccgcgagg aagaggcccg ggagcgcgcg cgtctcgagc gtgtcctcga gctgctccgg
     2521 gtagacgacg tcgtactcga cggcataggc cggccggagg atctccgccc gctcgagccc
     2581 cggaaggctc cggacgatct cgacctgaag atcggccggc agcgacatcg acaacccgcc
     2641 gagataggtc cactccgtac cgtgcccttc gggttcgacg aagacggtgt gccgttccct
     2701 ctccggaaag cggacgacct tgtcctcgat agaggggcaa taccgtggcc cacgcccctc
     2761 gatccgcccg gagtagagcg gggaacgccc gaggttcgtc cggatcagat cgtgcgtccg
     2821 ttcgttcgtg tggctcaacc agcaatcgac ctgcggcagg ctcgggaacg gttcgctccg
     2881 gcttcggagc gagaaccggc ggggccgctc gtctccggcc gaccgaagca ttttggtgcg
     2941 atcgattgat ttgccgtgta ccctcggcgg agtgcccgtc ttcatccgcc ccgtccgaag
     3001 gccgagcgcc cggagtcctt cggaaagcgc ggagcagggc gcctctcccc accgccccgc
     3061 ctcctctcgc cgctccccga cgtggacgag cccgcgcagg aacgttccgc tcgtcacgac
     3121 ggcggagagg cagaggatct cggcgccgtc ctcgaggcgc agcccggcga ggcgcccgcc
     3181 ccggacgagg aatccggcgg ccgtcccctc cgcgatcgtc aggttcggct gccgcgagag
     3241 gtgatcgacg accgccgatg cgtaggcccg ccggtccgac tggcagcgaa ggccctggac
     3301 ggccggtccg cgcgaggcgt tcaggacctt gaactggatc ccggtccggt cggccacccg
     3361 ggccatcacc ccgccgagcg cgtcgatctc gcggacgacc tgtcccttcg cgagaccgcc
     3421 gatcgccggg ttgcagctca ttctcccgat cgcggcccgc tcgcgcgtca ggaggagcgt
     3481 cctcttcccg aggcgagagg cggccgcggc cgcttccgct ccggcgtgcc cgcccccgac
     3541 gacgacgacc tcgtaccgct ccctcatttc ccgacgcaga acctttcgaa gatcctctcg
     3601 aggagctcct ccgtcgccgt ctcgcccgtg atctctccga gcgcgtggag cgcggcgcgg
     3661 accccggcgg ccgcgagctc tgccgggagc tcctcgccca gcggccggag cgcctccgcc
     3721 gcccgccgaa gcgcttcgcc ctggcgcggc agcggccccc agactcccgc ttccggaccg
     3781 gccccgagcc gccgcgaaat ctcctcccgg agcgtctcca gcccgcgtcc cgtgcgggcg
     3841 ctcaccggca gagcaccctc ctcgcacgcc gcgcccaggt cggccttcgt cgcgaccaga
     3901 agcaccggaa ctccctccgg gagccgctcc tgctcgcccg gggatgcgtc gggcgcgcga
     3961 acgaggagga ggaggtctgc gctcgcggcc gcgcgccggg ccgtctccac gccgagccgc
     4021 tccaccagct cctcggcctg ccggagcccg gcggtgtcga tcaggcggac cctctcgccg
     4081 cg
//
LOCUS       DAOTEZ010000399         4075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_16214_length_4075_cov_6.190547, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000399 DAOTEZ010000000
VERSION     DAOTEZ010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16214_length_4075_cov_6.190547"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="P5164_16635"
     CDS             <1..634
                     /locus_tag="P5164_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017850755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRY45564.1"
                     /translation="YTDPEKKHKGMSGFLVPRDTPGITVGKKEQNLGQRASDTRGLTF
                     EEVKVPAKYRLGNEGDGFRIAMAAFDHTRPPVASGAVGLAQRAMDESVKYAKERKTFG
                     LPIAAYQSISFMIAEMAMNIEAGRHLVRLAAWAIDAGKRNTKYAAMAKAFCADMAMKV
                     ATDAVQVHGGYGYSHEYPVEKLMRDAKIYQIYEGTSQIQRLIIAKETFER"
     gene            649..2130
                     /locus_tag="P5164_16640"
     CDS             649..2130
                     /locus_tag="P5164_16640"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HRY45565.1"
                     /translation="MGALGERAAVVDRSGRTTYGGLLASSDAVASALLAGRASLEGER
                     VAVLAEAGAPFVAALLGAWRAGGVAVPLALPHPPAEHAHVLDDSGATLALADVENAGR
                     LAPLAAERGARLLPIEAAARPAAFRRSGRSPSPGDAALILYTSGTTGKPKGAVHTHGS
                     LAAQVASLSAAWEWSADDAISNVLPLHHTHGLVNVTLCALANGARVEMLPGFDAEACW
                     RRLEEGGLTLFMAVPTVYARLAAAWEAADAAARARRSDACRGLRLMVSGSAALPVPLF
                     ETWERLSGHRLLERYGMTEIGMALGNPLRGERVPGTVGAPMPSVEVALVDESGGACPD
                     GAPGELRVKGPTLFREYHGRPEETANAFADGWFLTGDVAACEAGRFRILGRASSDILK
                     TGGYKVSALEIEDALRLHPVVMDAAVVGLPDEEWGERVAAAVVTGGAPLSLDSLRSFA
                     RERLAPYKLPTRLLVLDDLPRNAMGKVTKAAVRTLFGAPSSGR"
     gene            2145..3746
                     /locus_tag="P5164_16645"
     CDS             2145..3746
                     /locus_tag="P5164_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRY45566.1"
                     /translation="MPYLVRSSSAGAPDERFPLKPGGNTVGRSRENDVVLLDASLSRV
                     HARVDVEPDGSSVLDLGSRNGTFVGGLRVLQCRLKHGDTLQLGELPLRFEEETSPARL
                     GYSSPEMTLHGLIGAGAEIDKTSAIQLRGALPMQRSEAKLKILLTVSQILSSPEPVDA
                     VLPRIVELLLQILDVHRVALLLQEDGSDEPVAKVVRSRKTMAESDSFFSRSIVRHVFE
                     RGEGLVSDDALVDPRFAGSATVAGDSIRSSMAAPLKAGERVIGVLYVDHRSIPNRYSR
                     EDLEFLGAFGSQAAQAIENARLTAKLQEEAVRRSSLLRFFPPSVVGPLMGSADFGARV
                     RDADVTVLFSDISGFTAMSSELAPREVVDLLNRYFPVMADIVFRHEGTLEKYIGDALM
                     AIWGVPAPHPDDADRALSAALEMRRALERLNAAWGEGRRLEIHVGLNSGPVAFGNIGS
                     PQYVQFAAIGDTTNVSARVCTAAQPGEVLISEATRRRLTTSRFALEPLPPVAVKGKAE
                     PLLLHRARWAAAEETREIERGVPGA"
     gene            complement(3796..>4075)
                     /locus_tag="P5164_16650"
     CDS             complement(3796..>4075)
                     /locus_tag="P5164_16650"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRY45567.1"
                     /translation="LEELTGREVRTVVLGHLLRGGTPSSFDRLLALRFGAAAVRALEE
                     GQSGIMVALAPPTVNYVPLEDATSRMKSVPLDCDTIKTARDLAISFGD"
BASE COUNT          551 a         1456 c         1510 g          558 t
ORIGIN      
        1 ctacacggac cccgagaaga agcacaaggg gatgtccggc ttcctcgtcc cgcgcgacac
       61 gcccggaatc accgtcggca agaaggagca gaacctcggg cagagggcga gcgacacgcg
      121 cggcctgacg ttcgaggagg tgaaggtccc cgcgaagtac cgcctcggga acgaggggga
      181 cggcttccgg atcgcgatgg cggcgttcga ccacacccgc ccgcccgtcg cgtccggggc
      241 cgtcggcctg gcgcagcggg cgatggacga gtcggtgaag tacgcgaagg agcggaagac
      301 gttcgggctc ccgatcgccg cctaccagtc gatcagcttc atgatcgcgg agatggcgat
      361 gaacatcgag gcggggcgcc atctcgtccg cctcgccgcc tgggcgatcg acgccgggaa
      421 gcggaacacg aagtacgcgg cgatggcgaa ggccttctgc gccgacatgg cgatgaaggt
      481 cgcgacggac gccgtgcagg tccacggcgg ctacggctac tcgcacgagt acccggtcga
      541 gaagctgatg cgcgacgcga agatctacca gatctacgag gggacgagcc agatccagcg
      601 gctcatcatc gccaaggaga cgttcgagcg ctgagcctcc tcgagaggat cggcgcgctc
      661 ggggagcggg ccgccgtcgt cgaccgcagc gggcggacga cgtacggcgg cctcctcgcg
      721 tcgtcggacg ccgtcgcctc ggcgctcctg gccgggcgcg cctcgctcga gggggagcgc
      781 gtcgcggtcc tcgccgaggc cggcgccccc ttcgtcgcgg cgctcctcgg cgcctggcgc
      841 gccgggggcg tcgcggtccc gctcgcgctc ccgcacccgc cggccgagca cgcgcacgtc
      901 ctcgacgact cgggcgccac gctcgcgctc gccgatgtcg agaacgcggg acgcctcgcg
      961 cccctcgcgg cggagcgggg agcccggctc ctcccgatcg aggcggcggc gcggcccgcc
     1021 gcgtttcggc ggtcggggcg ttccccgagc ccgggagacg ccgccctgat cctctacacg
     1081 agcgggacga cggggaagcc gaagggggcc gtccacacgc acgggagcct cgcggcgcag
     1141 gtcgcgtcgc tctccgcggc gtgggagtgg agcgcggacg acgcgatctc gaacgttctc
     1201 ccgctccacc acacgcacgg cctcgtgaac gtaacgctct gcgcgctcgc gaacggggcc
     1261 cgcgtcgaga tgctccccgg cttcgacgcg gaggcgtgct ggcggcgcct cgaggagggc
     1321 ggcctcacgc tcttcatggc cgtcccgacc gtctacgcga ggctcgccgc ggcgtgggag
     1381 gcggccgacg ctgcggcgcg cgcgcgccgc agcgacgcct gccgcgggct ccggctgatg
     1441 gtctccggct ccgcggcgct ccccgtcccg ctgttcgaga cgtgggagag gctctcgggt
     1501 caccggctcc tcgagcgcta cggcatgacc gagatcggga tggcgctcgg gaacccgctc
     1561 cggggcgaga gggtcccggg gacggtcggg gcgccgatgc catccgtcga ggtggctctc
     1621 gtggacgagt cgggcggggc gtgccccgac ggcgcgcccg gcgagctgcg cgtgaagggg
     1681 ccgacgctct tccgggagta ccacgggcgg ccggaggaga cggcgaacgc gttcgccgac
     1741 ggctggttcc tcacgggcga cgtcgcggcg tgcgaggcgg ggcggttccg gatcctcggc
     1801 cgcgcctcga gcgacatcct gaagacgggg ggctacaagg tctcggcgct cgagatcgag
     1861 gacgccctgc ggcttcaccc tgtcgtgatg gacgccgcgg tcgtgggcct ccccgacgag
     1921 gagtggggcg agcgcgtcgc ggctgcggtc gtgacgggcg gcgcgccgct ctccctcgac
     1981 tccctccggt cgttcgcccg cgagcggctc gctccctaca agctcccgac gcgcctgctc
     2041 gtcctcgacg acctcccgcg gaacgcgatg gggaaggtga cgaaagcggc cgtccggacg
     2101 ctcttcggcg ctccgtcctc cggccgctag aatccctcgc agcgatgccc tatctcgtac
     2161 ggtcctcgag cgccggtgct cctgacgagc gctttccgct gaagccgggc ggcaacaccg
     2221 tcgggcgttc ccgcgagaac gacgttgtcc tcctcgacgc gagcctgtcg cgggtccacg
     2281 cccgggtcga cgtcgagccg gacgggtcga gcgtcctcga cctcgggagc cggaacggca
     2341 cgttcgtcgg cgggctccgc gtcctccagt gccggctcaa gcacggagac acgctgcagc
     2401 tcggggagct ccctctccgt ttcgaggagg agacgtcccc ggcccggttg ggctactcct
     2461 ccccggagat gacgctccac ggcctgatcg gggcgggtgc ggagatcgac aagacgtcgg
     2521 cgatccagct ccgaggagcg ctgccgatgc agcgctccga ggcgaagctg aagatcctgc
     2581 tgaccgtctc gcagatcctc tcctcgcccg agccggtcga cgcggtcctg ccgcggatcg
     2641 tcgagctcct cctccagatc ctcgacgtcc accgcgtcgc gctcctcctc caggaggacg
     2701 ggtcggacga gccggtcgcg aaggtcgtgc ggtcgcggaa gacgatggcg gagagcgatt
     2761 cgttcttcag ccggagcatc gtgcggcacg tcttcgagcg gggggagggg ctcgtcagcg
     2821 acgacgcgct cgtcgacccg cgtttcgccg gctcggcgac cgtcgccggc gactccatcc
     2881 gctcctcgat ggcggcgccg ctgaaggcgg gcgagcgggt gatcggcgtc ctctacgtcg
     2941 accaccgctc gatcccgaac cgctactcga gggaggacct cgagttcctc ggcgcgttcg
     3001 gcagccaggc cgcccaggcg atcgagaacg cgcgcctgac ggcgaagctc caggaggagg
     3061 ccgttcggcg ctcgagcctc ctccgcttct tcccgccgtc cgtcgtcgga cccctcatgg
     3121 gctcggccga cttcggcgcc cgggtgcgcg acgccgacgt gaccgtactc ttctcggaca
     3181 tcagcggctt cacggcgatg tcctcggagc tggcgccccg ggaggtcgtc gacctcctga
     3241 accgctactt ccccgtcatg gccgacatcg tcttccgcca cgaggggacg ctcgagaagt
     3301 acatcggcga cgcgctgatg gcgatctggg gggtgccggc gccgcatccg gacgacgccg
     3361 accgggccct ctcggccgct ctcgagatgc gccgcgcgct cgagaggctg aacgccgcct
     3421 ggggcgaggg gcggcgtctc gagatccacg tcggcctgaa ctccggcccg gtcgcgttcg
     3481 ggaacatcgg ctcgccgcag tacgtccagt tcgcggcgat cggcgacacg acgaacgtct
     3541 cggcgcgcgt ctgcacggcg gcgcagccgg gggaggtgct gatctcggag gcgacccgcc
     3601 ggcggctgac gacgtcgagg ttcgcgctcg agccgctgcc tccggtcgcg gtcaagggga
     3661 aggcggagcc gctcctcctc caccgggccc gatgggcggc ggcggaggag acgcgggaga
     3721 tcgagcgggg cgttccggga gcgtgacccg ggccggccgc ggacggcacg gagccgccgc
     3781 gctgccgccc gcggcctagt ctccgaagct gatggcgagg tcccgcgcgg tcttgatcgt
     3841 gtcgcagtcg agcgggacgg acttcatccg gctcgtcgcg tcctcgagcg ggacgtagtt
     3901 caccgtcggc ggggcgaggg cgaccatgat gccgctctgc ccctcctcga gcgcgcggac
     3961 ggcggccgcg ccgaagcgga gcgcgaggag acggtcgaag gaggacggag tcccgccgcg
     4021 caggaggtgg ccgaggacga cggtccgcac ctcgcgtccc gtcagctcct cgagc
//
LOCUS       DAOTEZ010000400         4056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_16311_length_4056_cov_5.388903, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000400 DAOTEZ010000000
VERSION     DAOTEZ010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16311_length_4056_cov_5.388903"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="P5164_16655"
     CDS             <1..245
                     /locus_tag="P5164_16655"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRY45568.1"
                     /translation="FLAIWGLVVVSTLADNVLKPILIGGGTQLSTLVVFLGVFGGLAA
                     FGILGLFIGPMVLAFALTLVEILREAVHPKAEPDAA"
     gene            complement(253..1395)
                     /locus_tag="P5164_16660"
     CDS             complement(253..1395)
                     /locus_tag="P5164_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45569.1"
                     /translation="MRRDVLLLLSAAALLGPQACDSARPPAADVTTAPSPAAQDAPAR
                     RAGPWEGVLSPLQWPGERHLANVRQLTYGGENAEAYWSADGKKLVFQATRPPFRCDQI
                     FAIDADDPGEPRLVSTGKGRTTCAYWFPDGERILFSSTHLGGGPLCPPPPDMSSGYVW
                     SVNPDYEIFSARPDGSGLRRLTASAGYDAEATISTDGRRVVFTSARDGDLDLYSMNAE
                     GGDVRRLTRTPGYDGGAFFSDDGEWIVYRASRPETPQLVEEFQSLLRRHLVRPSRLEI
                     RMMRADGSDDRQVTSHGVASFAPFFFRGGHDRILFVSNLADPGGRDFDIWAVRSDGTD
                     LERVTYNPTFDGFPMFSPDGRRLAFCSNRHNAKPGETNVFVADWVP"
     gene            1419..>1792
                     /locus_tag="P5164_16665"
     CDS             1419..>1792
                     /locus_tag="P5164_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45570.1"
                     /translation="MTNDRRGDAAGLARELAAQLAALATPGPNTPLSSRHGPSPQGVP
                     SLAGPRAILENASRFAQPVLPADAPFRSLKALVLRALRIVTRDQGVFNSALHEALRTA
                     LLEIDGALGALAKAQQESRAEL"
     assembly_gap    1793..1892
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1893..2859
                     /locus_tag="P5164_16670"
     CDS             <1893..2859
                     /locus_tag="P5164_16670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY45571.1"
                     /translation="DGIGREAGAREALGREVTGSQARIDLLERRSDGVLGRLQESTSR
                     LEAAEARLERLQADLSRASEDLRAARLEWTTLRTELRSGAPLVSPDGEPASGPESRSP
                     SDPISAGLYADFERAFRGAESEIRRRQDGDVALFAGSRLPIADLGCGRGEFLEALRAR
                     GLSGLGCDTNPVMVARCREKGLAVDEADLFGWLRGRAVDSLGGVTAYQVVEHLPADAL
                     LPFVELAVSRLAPGGRLLLETVNAESVYAMKWFWMDLTHVRPVPAPSLGRLMEACGLR
                     SVTIAHRSPVPREESGGGVEPSDPLVAALARLVFAPQDVAVTGVK"
     gene            2860..3897
                     /locus_tag="P5164_16675"
     CDS             2860..3897
                     /locus_tag="P5164_16675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY45572.1"
                     /translation="MANVLVCTAQVPFASGGAERHAAGLVRELRARGHRVDLVQLPFK
                     WYPREEILLSAAAWRLVDVTEADGTKVDLVVPMKFPSYLVRHPNKVVWLIHQFRQAYD
                     RFGTEQSDFTASPEDTRWRELIAEADATGLGEARKVFTNARNTADRLMRFNGIEAEAL
                     YHPPPLAGRYRDDGPGRWALVVGRLDPWKRMDLAIEGAASGGFPLVIAGCGPDEERLR
                     KVAARTGADVDFRGAVSDDELLALYAGCGAVLFTPADEDYGYIALEAFLSKKPVVTCT
                     DSGGPLEFVVDGETGRVAPPSGAAVGEAASALLAAPEAARRMGERGYDAVRGISWDAV
                     VTSLLRAGGGG"
BASE COUNT          509 a         1419 c         1478 g          550 t
ORIGIN      
        1 tcttcctcgc gatctggggc ctcgtcgtcg tctcgacgct ggccgacaac gtcctgaagc
       61 cgatcctgat cggcggcggg actcagctct cgacgctggt cgtcttcctc ggcgtcttcg
      121 gaggcctcgc cgccttcggg atcctcggcc tcttcatcgg gccgatggtc ctcgccttcg
      181 cgctgacgct cgtcgagatc ctccgcgagg cggtccaccc gaaggcggag ccggacgccg
      241 cgtgacgcga cgtcacggca cccagtcagc gacgaagacg ttcgtctccc cgggcttcgc
      301 gttgtggcgg ttcgagcaga acgcgagcct tcggccgtcg ggggagaaca tcgggaagcc
      361 gtcgaacgtc gggttgtagg tgacccgctc gaggtcggtc ccgtcggagc ggacggccca
      421 gatgtcgaag tcacggccgc cggggtcggc gaggttcgac acgaagagga tccggtcgtg
      481 ccctccccgg aagaagaacg gcgcgaagct cgcgacgccg tggctcgtca cctgccggtc
      541 gtcggagccg tcggcgcgca tcatccggat ctcgaggcgg ctcggccgga cgaggtgccg
      601 gcgaaggaga gactggaact cctcgacgag ctgcggcgtc tcggggcgcg aggcgcggta
      661 gacgatccac tcgccgtcgt cgctgaagaa cgccccgccg tcgtacccgg gcgtccgggt
      721 cagccgacgg acgtcgccgc cttcggcgtt catggagtag aggtcgaggt ccccgtcccg
      781 cgccgacgtg aagacgaccc tccggccgtc cgtcgagatc gtcgcctcgg cgtcgtatcc
      841 ggcgctggcc gtgaggcgcc ggagcccgga gccgtcgggg cgggcgctga agatctcgta
      901 gtccggattc acgctccaga cgtagcccga ggacatgtcg ggcggagggg ggcagagcgg
      961 cccgccgccg aggtgggtcg acgagaagag gatccgctcc ccgtcgggga accagtacgc
     1021 gcaggtcgtg cgccccttgc cggtcgagac gaggcggggc tcgcccgggt cgtccgcgtc
     1081 gatcgcgaag atctggtcgc agcggaacgg ggggcgagtc gcctggaaga cgagcttctt
     1141 cccgtcggcc gaccagtacg cctcggcgtt ctcccctccg tacgtcagct ggcggacgtt
     1201 cgccaggtgc cgctctcccg gccactggag cggcgagagg acgccctccc agggacccgc
     1261 ccggcgtgcc ggagcgtcct gagcggcggg cgacggggcg gtggtcacgt cggcggccgg
     1321 cggccgcgcc gagtcgcacg cctgagggcc gaggagagcg gcggcggaga ggaggagcag
     1381 gacgtcgcgg cgcatgggtg ggccgtaata taaggcggat gacgaacgac cgacgcggcg
     1441 acgcggcggg cctcgcgcgc gagctcgccg cgcagctcgc cgcgctcgcg acacccggac
     1501 cgaacacgcc cctctcttcg cggcacggac cgtcgccgca gggcgtcccg agcctcgcgg
     1561 gtccgcgagc gatcctcgag aacgcctcgc gcttcgccca gccggtcctc ccggcggacg
     1621 ccccgttccg aagcctgaag gcgctcgtcc tgcgcgccct ccggatcgtc acgcgcgacc
     1681 agggggtctt caactcggcg ctccacgagg cgctgcggac ggcgctcctc gagatcgacg
     1741 gggcgctcgg cgcgctcgcg aaggcgcagc aggagagccg cgcggagctc gannnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggacggga tcggccggga ggcgggcgcc
     1921 cgggaggccc tcggccgcga ggtgacggga agccaggcgc ggatcgacct cctcgagaga
     1981 cggtccgacg gcgtgctcgg acgccttcag gagtcgacct cccgtctcga ggcggcggag
     2041 gcacggctcg agaggctcca ggcggacctc tctcgcgcct cggaagacct ccgggcggcg
     2101 cgcctcgagt ggacgacgct ccggaccgag ctccgaagcg gcgcccctct cgtctctccg
     2161 gacggcgagc cggcgtccgg gccggagtcg cggtccccgt ccgacccgat ctccgcgggt
     2221 ctctacgcgg acttcgagag ggcgttccgg ggagcggaat cggagatccg gcggcggcag
     2281 gacggggacg tggccctctt cgccggaagc cggctcccga tcgccgacct cggctgcggt
     2341 cggggagagt tcctcgaggc gctgcgcgcg cgcggtctgt ccggcctcgg ctgcgacacg
     2401 aacccggtca tggtcgcgcg ctgccgcgag aaggggctcg cggtcgacga ggccgatctc
     2461 ttcggatggc ttcgcgggcg cgcggtcgac tccctcggcg gcgtgacggc gtaccaggtc
     2521 gtcgagcatc tcccggccga cgcgctcctg ccgttcgtcg agctcgccgt ctcgcgcctc
     2581 gcgccgggcg ggcggctcct tctcgagacg gtgaacgccg agtcggtcta cgcgatgaag
     2641 tggttctgga tggacctcac gcacgtccgg cccgtcccgg caccgtcgct cggacgcctg
     2701 atggaggcct gcggcctgcg aagcgtcacg atcgcccacc ggtcccccgt tccccgcgaa
     2761 gagtcgggcg gcggcgtgga gccctccgac ccgctcgtcg ccgccctggc gcggctcgtc
     2821 ttcgcgccgc aggacgtcgc ggtgacgggg gtgaagtagg tggcgaacgt cctcgtctgc
     2881 acggcccagg tcccgttcgc ctcgggaggc gccgagcgac acgccgccgg cctcgtccgg
     2941 gagctgcggg cccgcggaca tcgcgtcgac ctcgtccagc tcccgttcaa gtggtacccg
     3001 cgggaggaga tcctcctttc cgccgcggcg tggcggctcg tcgacgtcac ggaggccgac
     3061 gggacgaagg tggacctcgt cgtcccgatg aagttcccgt cgtacctcgt tcggcatccg
     3121 aacaaggtcg tctggctgat ccaccagttc cgccaggcct acgaccgctt cgggacggag
     3181 cagagcgact tcacggcgag ccccgaggac acgcgctggc gggagctgat cgccgaggcc
     3241 gacgcgacgg ggctcggcga ggcgcgcaag gtcttcacga acgcgcgcaa caccgccgat
     3301 cgcctgatgc gcttcaacgg catcgaggcc gaggcgctct accatccgcc gccgctcgcg
     3361 gggcgctacc gcgacgacgg cccgggccgc tgggctctcg tcgtcggccg cctcgacccc
     3421 tggaagcgga tggacctcgc gatcgaggga gcggcgtccg gagggttccc gctcgtgatc
     3481 gcgggctgcg gccccgacga ggagcgcctg cggaaggtcg ccgcccggac cggcgccgac
     3541 gtcgacttcc ggggggccgt ctccgacgac gagctcctgg cgctctacgc cggctgcgga
     3601 gccgtcctgt tcacgccggc cgacgaggac tacggctaca tcgctctcga ggcgttcctc
     3661 tcgaagaagc cggtcgtcac ctgcacggac tcgggcgggc cgctcgagtt cgtcgtcgac
     3721 ggtgagacgg ggcgcgtcgc gccgccgagc ggcgcggccg tcggcgaggc ggcgtccgcg
     3781 ctcctcgccg cgccggaggc cgcgcggcgg atgggggagc gcgggtacga cgcggtccgc
     3841 ggcatctcct gggacgcggt ggtgacgtcg ctcctccgcg cgggcggcgg cgggtgaacg
     3901 tcgcgccgct cggcttcgtc agcccgctcc cgccggttcg ctcgggcatc gcggactact
     3961 cggccgacct cctcgcggcc cttcgcaagg aggtggagct cgagacctac ggcccgtccg
     4021 aggccgccgc ggccttccga cacgggcacg gtgccg
//
LOCUS       DAOTEZ010000401         4040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_16390_length_4040_cov_5.557089, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000401 DAOTEZ010000000
VERSION     DAOTEZ010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16390_length_4040_cov_5.557089"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="P5164_16680"
     CDS             complement(<1..935)
                     /locus_tag="P5164_16680"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HRY45573.1"
                     /translation="MRLAILGTRGVPAAYGGFETLAEELSARLAERGHEVTVYARRGA
                     VRERVDAWRGVRVVFAPTLRHKYLDTFVHGVTSGLHAAVAAYDAVLFCNGANALACRL
                     PRLLGAGTRVLLNVDGLERNRRKWNRLGRAVYALSERLSCVLPDVVVTDAEEIRRYYR
                     ERYGAESAFVPYGSDLPEPEDETVLGSLGLEPGRYLLYVSRFEPENNPDAVVRAYRDV
                     PGEVPLLLVGSAPYAEGFIARLKEEAARDPRVVLPGAIYGEGYRALLANAAAYVHATE
                     VGGTHPALIEAMGYGRPVLVHDTPENREVAGDAAL"
     gene            complement(932..>1432)
                     /locus_tag="P5164_16685"
     CDS             complement(932..>1432)
                     /locus_tag="P5164_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY45574.1"
                     /translation="CVALYRAAALADAKISTGFFDDDFFVYREDVDLAWRLRGRGWTA
                     RCVPSAVAWHRRRNLPERRREMSAVANLHSVKNRFLLRINNAGPDHLRATFPLTFARD
                     VVVVGACVFRERTSLEALRWLARNRERLLVKRREVLDRRTVSDRALLRWFGPDPGGAR
                     IPDVDA"
     assembly_gap    1433..1532
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1533..1816)
                     /locus_tag="P5164_16690"
     CDS             complement(<1533..1816)
                     /locus_tag="P5164_16690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY45575.1"
                     /translation="MPRVVVSLVTHNESRDAERLLPTLFAQTFRDFALVAVDNRSEDG
                     TRSALAAAQKTAPVPMEIVASRENLGFAGGHNVGIAKAIERGAEWVLVLNA"
     gene            complement(1817..2776)
                     /locus_tag="P5164_16695"
     CDS             complement(1817..2776)
                     /locus_tag="P5164_16695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY45576.1"
                     /translation="MSGSLARVRAIVVSWNSAGHVGRAVASVPPPTPVVVVDNASTDG
                     SAEEAERAGAVVLRRTTNAGFGPACNLGAGPVDGLPDAEALLFLNPDAALVDGPAALA
                     ALLAELDADPAVAAVAPALEGDGQEEFQLRKLPTLASLSREAFLVNRLFPSNRGFRRE
                     RYLDRDRTRPFDVEQPAAAALLVRRTAFERVGGFDPAFAPAWFEDVDLSAKLLESGYR
                     IRFVPAARASHVGGTTMRALPYDDYLPLYTRNLLRYLGRHSGPATRAGARSVLAAGAL
                     LRLALLPIVRGDHARPEAARAYVRVLRGLAGLGFRSALLPRES"
     gene            complement(2773..3696)
                     /locus_tag="P5164_16700"
     CDS             complement(2773..3696)
                     /locus_tag="P5164_16700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY45577.1"
                     /translation="MERLPVGVVVVNWNGGALLDSCLDALSDEPASRVLVVDNGSEAE
                     ELRRLAARPGVFVLPLGVNRGFASAANGGAVDARLKSLPYLAFVNNDAVLEPGYLASC
                     VAALEADPGLAAVQGVILDGEGRLADGLGIAWNRRLEAVQLGHGEPPPPAGAPPFPIP
                     GVSGTAPVFRRRAFEEAGGFAASFFAWYEDADLALRLLRSGARFACVPAARARHVGSA
                     TGRRVPERKWRLLFGNRLRTLRRNLSASARLSAFLTHPVPLPALRDAVRELGAGRALR
                     TFAAATAAVTRRSAEDRAARRGPPLLRSLPR"
     gene            3735..>4040
                     /locus_tag="P5164_16705"
     CDS             3735..>4040
                     /locus_tag="P5164_16705"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF014458.2"
                     /note="catalyzes a two-step reaction, first charging a
                     serine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRY45578.1"
                     /translation="MLARDFLRTRFPDLPHLLRNRTYDAGALARWGELDAERRRLLTS
                     VEALKAERNTLSAEVGRKKKAKEDATAEMERAKALGGEVAAGEKRVEEIEGEFFAIEQ
                     "
BASE COUNT          568 a         1376 c         1562 g          434 t
ORIGIN      
        1 aagagggcgg cgtcgcccgc cacctcgcgg ttctcgggag tgtcgtgaac gaggaccggc
       61 cggccgtagc ccatcgcctc gatgagcgcg gggtgcgtcc cgcccacctc ggtcgcgtgg
      121 acgtaggccg ccgcgttcgc gaggagggcg cggtagccct cgccgtagat cgcgccgggg
      181 aggacgaccc gggggtccct cgccgcctct tccttcaggc gcgcgatgaa cccctcggcg
      241 tacggggcgg agccgacgag gaggaggggg acctctcccg ggacgtcgcg gtaggcgcgg
      301 acgacggcgt cggggttgtt ctccggctcg aagcgggaga cgtagaggag gtaccgcccc
      361 ggctcgagac cgaggctccc gagcaccgtc tcgtcttccg gctcgggaag gtcggagccg
      421 tacgggacga acgccgactc ggcgccgtag cgctccctgt agtaccgccg gatctcctcc
      481 gcgtcggtca cgacgacgtc ggggaggacg caggagagcc gctccgagag cgcgtagacc
      541 gctctcccga ggcgattcca cttccgccgg ttcctctcga ggccgtcgac gttcagaagg
      601 acccgcgtcc cggcgccgag gaggcggggg aggcggcagg caagcgcatt cgcgccgttg
      661 cagaagagga cggcgtcgta cgccgcgacc gcggcgtgga gccccgacgt cacgccgtgg
      721 acgaacgtgt cgaggtactt gtgccggagc gtcggcgcga agacgacgcg gacgccgcgc
      781 cacgcgtcga cccgctcgcg gaccgcaccc cttcgggcgt acaccgtgac ctcgtgcccg
      841 cgttccgcga ggcgcgcgga gagctcctcg gcgagcgtct cgaacccgcc gtacgccgcc
      901 gggacgccgc gcgtcccgag gatcgcgagc ctcacgcgtc cacgtccggg atccgcgcgc
      961 ctcccgggtc cggcccgaac cagcggagga gcgcccggtc ggagacggtc cggcggtcga
     1021 gcacctcgcg gcgcttcacg aggagccgct cccggttccg ggcgagccag cggagcgcct
     1081 cgagggaggt cctctcccgg aagacgcagg ctccgacgac gaccacgtcc cgcgcgaacg
     1141 tgagggggaa tgtcgcacgg aggtggtccg ggccggcgtt gttgatccgg aggaggaacc
     1201 ggttcttcac cgagtggagg ttcgcgacgg ccgacatctc ccgccgccgc tccgggaggt
     1261 tccggcgccg gtgccaggcg acggccgagg ggacgcagcg ggccgtccag ccccgcccgc
     1321 ggaggcgcca ggcgaggtcg acgtcctccc ggtagacgaa gaagtcgtcg tcgaagaagc
     1381 cggtcgagat cttcgcgtcg gcgagggccg cggcgcggta gagggcgacg cannnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcgttcag gacgaggacc cactcggccc
     1561 cccgctcgat cgccttcgcg atcccgacgt tgtgcccccc tgcgaagccc aggttctcgc
     1621 gcgaggcgac gatctccatc ggcacggggg ccgttttctg cgcggccgcg agcgcggagc
     1681 gcgtgccgtc ctccgagcgg ttgtcgacgg cgacgagggc gaagtcgcgg aacgtctgcg
     1741 cgaagagcgt cgggaggagc cgctcggcgt cgcgggactc gttgtgcgtg acgagggaga
     1801 cgacgacgcg cggcactcag ctctcccgcg gcaggagcgc gctccggaag ccgagcccgg
     1861 cgaggcctcg caggacgcgg acgtacgccc gcgccgcctc cgggcgggca tggtccccgc
     1921 ggacgatcgg gaggagggcg aggcggagga gggcgcccgc ggcgaggacg gaccgcgccc
     1981 cggcacgcgt ggccggcccg gaatggcgcc ccaggtaccg gaggaggttc cgcgtgtaga
     2041 gcggaaggta gtcgtcgtag ggaagcgcgc gcatcgtcgt cccgcccacg tggctcgcgc
     2101 gagccgcggg gacgaagcgg atacggtacc ccgattcgag gagcttcgca gagaggtcga
     2161 cgtcctcgaa ccaggcgggg gcgaacgcgg ggtcgaagcc gcccacccgc tcgaacgcgg
     2221 tgcgacgcac gagaagcgcg gcggccgcgg gctgctccac gtcgaacggg cgcgtccggt
     2281 cccggtcgag gtagcgctcc ctgcggaagc cccggttcga cgggaagagg cgattcacga
     2341 ggaacgcctc ccgggagagc gacgcgagcg tcggcagctt ccggagctgg aactcctcct
     2401 gcccgtctcc ttccagggcg ggcgcgacgg ccgcgacggc cgggtcggcg tcgagctcgg
     2461 cgagcagcgc ggcgagcgcg gccgggccgt cgacgagggc ggcgtcgggg ttcaggaaga
     2521 ggagcgcctc ggcgtcgggg agcccgtcca cgggcccggc cccgaggttg cacgccgggc
     2581 cgaagccggc gttcgtcgtc cggcgaagga cgacggcgcc cgcccgctcg gcctcctcgg
     2641 ccgagccgtc ggtcgaggcg ttgtcgacga cgacgaccgg cgtcggcggc gggaccgacg
     2701 cgacggcccg gccgacgtgc ccggccgagt tccaggagac gacgatcgcc ctcacccggg
     2761 cgagagagcc gctcaccgcg gaaggctccg caggagggga gggcctctcc gcgcggcacg
     2821 gtcctcggcc gagcggcgtg tcacggcggc cgtggcggcg gcgaacgttc ggagggcgcg
     2881 ccccgcgccg agctcgcgaa cggcgtcgcg gagggcggga agcggcacgg ggtgggtgag
     2941 gaaggcggag agccgcgcgg aggccgacag gttgcgccgg agcgtccgga ggcggttccc
     3001 gaagaggagc cgccacttcc gctcggggac ccggcgtccc gtcgccgagc cgacgtgacg
     3061 ggcccgggcg gcggggacgc aggcgaatcg cgctccggag cggaggagcc gcagggccag
     3121 gtcggcgtcc tcgtaccagg cgaagaagga ggcggcgaag ccgccggctt cctcgaacgc
     3181 ccggcgccgg aagacgggcg ccgtgccgga gacgccgggg atcgggaagg ggggcgcgcc
     3241 ggccggcggc ggcggctcgc cgtggccgag ctggacggcc tcgaggcgcc ggttccacgc
     3301 gatcccgagg ccgtcggcga ggcgcccctc tccgtccagg atcacgccct ggacggccgc
     3361 cagccccggg tcggcctcca gcgcggcgac gcacgaagcg aggtagcccg gctcgaggac
     3421 ggcgtcgttg ttgacgaagg ccaggtaggg gagcgacttc agccgggcgt cgaccgcccc
     3481 gccgttcgcg gcggaggcga agccccggtt caccccgagc gggaggacga agactccggg
     3541 gcgcgccgcg agccgtcgga gctcctccgc ttcggagccg ttgtcgacga cgaggacccg
     3601 cgacgccggc tcgtcggaga gcgcgtcgag gcaggaatcg aggagcgccc ccccgttcca
     3661 gttcacgacg acgaccccga cggggagcct ctccatccgg cggattatcg gggccgccgt
     3721 aaaatccgcc tcccatgctg gcccgagact tcctccgcac ccgcttcccc gacctgccgc
     3781 acctcctccg gaaccggacg tacgacgccg gcgccctcgc ccgctggggc gagctcgacg
     3841 ccgagcgccg caggctcctg acgtccgtcg aggcgctgaa ggccgagcgg aacacgctct
     3901 cggccgaggt ggggcggaag aagaaggcga aggaggacgc gaccgccgag atggagcggg
     3961 cgaaggccct cggcggggag gtcgccgccg gcgagaagcg ggtcgaggag atcgaggggg
     4021 agttcttcgc gatcgagcag
//
LOCUS       DAOTEZ010000402         4031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_16450_length_4031_cov_6.722082, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000402 DAOTEZ010000000
VERSION     DAOTEZ010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16450_length_4031_cov_6.722082"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1563)
                     /locus_tag="P5164_16710"
     CDS             complement(<1..1563)
                     /locus_tag="P5164_16710"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRY45579.1"
                     /translation="MSRTRPLLAPLLLRPLREDRGRFARTVAGIAVGVATMAAILLAN
                     ASVLSSFTETVDFVAGKAGLTVLADGPGIPEETLERLAWLRMKGVAIVPAVSATVALA
                     GGEGDVLELLGIDPVSDPSVRDYRFVAGTAGGTSLEKLFGIFRAGAVLVPEPLAERLG
                     LAPGAELDVLSGGATHRLRVAGVLRMDGAARAAAGSVLFADLATAQEVLGREGFLDRI
                     DLVLPAGLADADRTALEASVRDSLPAGVTVGRPERRTETVDRMVRSFRVNLTALGLVA
                     LLVGTYFVYNTLSISVLRRRSDIGTVRALGASARSVFLVFLAEGAALGALGGALGVAL
                     GALLARIALAAVGGTATDFYLPQAAPVFTLAPSALATSFLAGLFASILSALAPAVEAA
                     RVEPAATIRHGSVEASRRRAVAPLAVAGLLLLAFAALATRPGPVNGLPLFGFASVFLV
                     AAGVSLLAPLSVVTVARLADGPAVRLLGVEARIARANLSGSLSRTAVAVAALSMGISM
                     MVAVAFIVGSFRA"
     gene            complement(1560..2249)
                     /locus_tag="P5164_16715"
     CDS             complement(1560..2249)
                     /locus_tag="P5164_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY45580.1"
                     /translation="MGARVDVVEVRKSWGEGGSGGDGTVAEALAGVSFVLEPGELVAL
                     LGPSGCGKSTLLHLLGGLDRPTSGQVTVNGHDLARLSEADLDLYRRRTAATVFQLFNL
                     LPTMTALENVALPLLLDGVPASEADGRAERLLGEVGLAAKSGSFPWQLSGGQMQRVAV
                     ARALVASPALLLADEPTGSLDSRSGAQVLDLIAELASARGVTVLLATHSEPAAGRARR
                     VLRLRDGLLER"
     gene            2324..3130
                     /locus_tag="P5164_16720"
     CDS             2324..3130
                     /locus_tag="P5164_16720"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY45581.1"
                     /translation="MERLRDLWRHRALVGVLVTREVKARYRGSVLGFFWSLLNPLLML
                     AVYTLVFQLLLPNRSPSTSPYALFLFCGLLPWNWLSSAITDAASSLLTHGALLRKILF
                     PAEVLPAVAVLAQGVHFLLALPVLLAALVAGALGAFGAKVPLGWALLQVVPLLVLQAI
                     LLLGIGLFLAALTVHFRDVRDLLQTVLSVLFFATPILYTLNDLPKTKLTRILALNPFA
                     PLFAGWHDALFYGRVSPLQTWAAAAAISVAAFAVGFVFFDRLRDSFPEAV"
     gene            3037..3615
                     /locus_tag="P5164_16725"
                     /pseudo
     CDS             3037..3615
                     /locus_tag="P5164_16725"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT          550 a         1457 c         1491 g          533 t
ORIGIN      
        1 ggcgcggaac gagccgacta tgaaggcgac ggcgaccatc atcgagatcc ccatcgagag
       61 ggccgcgacg gcgacggccg tccgcgacag gctcccgctc aggttcgcgc gggcgatccg
      121 ggcctcgacg ccgaggagcc ggacggccgg gccgtccgcc aggcgcgcga cggtcacgac
      181 ggagagcggc gcgaggaggg agacgccggc ggcgacgagg aagaccgacg cgaacccgaa
      241 gagggggagg ccgttcaccg gtccgggccg ggtcgcgagc gcggcgaacg cgaggaggag
      301 gaggccggcc accgcgagcg gcgccacggc gcgccggcgc gaggcctcca ccgagccgtg
      361 ccggatcgtc gccgcgggct ccacccgggc ggcctcgacc gccggcgcga gggcggagag
      421 gatcgacgcg aagaggccgg cgaggaacga cgtcgcgagc gccgacgggg cgagcgtgaa
      481 gaccggcgcg gcctgcggga ggtagaagtc ggtcgcggtc ccgccgacgg cggcgagcgc
      541 gatccgggcc aggagcgctc cgagagcgac gccgagcgcc cctccgaggg cgccgagcgc
      601 ggccccttcc gcgagaaaga ccaggaagac cgagcgggcc gaggcgccaa gcgcacggac
      661 ggtgccgatg tcgctccgcc ggcgcaggac cgagatcgag agcgtgttgt agacgaagta
      721 ggtgccgacg aggagcgcga cgaggcccag ggccgtcagg ttgacccgga aggaacggac
      781 catccggtcg accgtctccg tccgccgctc ggggcgcccg acggtgaccc ctgccgggag
      841 cgaatcgcgg acggaggcct cgagggccgt tcggtcggcg tcggcgaggc cggccggaag
      901 gacgaggtcg atccggtcga ggaagccctc gcgcccgaga acttcctgcg ccgtcgcgag
      961 gtcggcgaag aggacggagc cggcggcggc acgcgccgcg ccgtccatcc gcaggacgcc
     1021 ggccacgcgc agccgatgcg tggcgccccc cgagaggacg tcgagctccg cgcccggagc
     1081 gaggccgagg cgctcggcga gcggctcggg gacgaggacc gctccggccc ggaagatccc
     1141 gaacagcttc tcgagcgacg tcccgcccgc cgtcccggcg acgaagcggt agtcccggac
     1201 ggaagggtcg gagaccgggt cgatgccgag gagctcgagg acgtcgccct cgcctccagc
     1261 gagggcgacc gtcgccgaga ccgccgggac gatcgcgacc cccttcatcc ggagccacgc
     1321 gagccgctcg agcgtctcct ccggaatccc cggtccgtcg gcgaggacgg tcagcccggc
     1381 cttcccggcc acgaagtcga ccgtctccgt gaaggacgag aggaccgagg cgttcgcgag
     1441 gaggatcgcg gccatcgtgg cgacgccgac ggcgattccg gcgacggtcc gcgcgaatcg
     1501 cccgcggtcc tcccggagcg gccggaggag aagcggggcg aggagcggcc gggtccggct
     1561 caccgctcga gcaggccgtc gcggaggcgc aggacgcgcc gcgcgcgccc ggctgcgggc
     1621 tccgagtgcg tcgcgaggag gaccgtcacg ccccgggccg acgcgagctc cgcgatcagg
     1681 tcgaggacct gcgcgccgct tcgcgagtcg aggctcccgg tcggctcgtc cgcgaggagg
     1741 agcgccggcg aggcgacgag ggcacgcgcg acggcgaccc tctgcatctg gccgcccgag
     1801 agctgccacg ggaaggagcc cgacttggcc gcgagcccga cctccccgag gagcctctcg
     1861 gcgcgtccgt cggcttcgga ggccgggacc ccgtcgagga gcagcggcag ggcgacgttc
     1921 tcgagagccg tcatcgtcgg gaggaggttg aagagctgga agacggtggc ggccgtccgc
     1981 cggcggtaga ggtcgaggtc cgcctcggag aggcgcgcga ggtcgtggcc gttcaccgtc
     2041 acctgccccg aagtcggccg gtcgaggccg ccgaggaggt ggaggagcgt cgacttgccg
     2101 caccccgacg gtccgaggag ggcgacgagc tccccgggct cgaggacgaa cgagactccc
     2161 gcgagcgcct cggcgacggt tccgtcgccc ccgctgccgc cttctcccca cgactttcgg
     2221 acctccacga cgtcgacgcg cgcgcccatg cgccgactct acggcgatcc ggtgcgcgga
     2281 gatggggagg cccgcgcggc gccgcgtatc atcggcggtc ggaatggagc ggctccgaga
     2341 cctctggcgc catcgggcgc tcgtcggcgt cctcgtgacg cgcgaggtca aggctcgcta
     2401 tcgcgggagc gttctcggct tcttctggtc gctcctcaac cccctcctga tgctggcggt
     2461 ctacacgctc gtcttccagc tcctcctccc gaaccgcagc ccctcgacct ccccctacgc
     2521 cctcttcctc ttctgcggcc tcctgccctg gaactggctc tcctcggcga tcaccgacgc
     2581 ggcttcttcg ctcctgacgc acggtgcgct cctgcgcaag atcctcttcc cggccgaggt
     2641 ccttccggcg gtggcggtcc tcgcgcaggg agtccacttc ctcctcgcgc tcccggtcct
     2701 cctggcggcc ctcgtcgccg gggcgctggg agctttcggc gcgaaggtcc cgctcggatg
     2761 ggcgctcctc caggtggtcc ctctcctcgt ccttcaggcg atcctcctgc tcgggatcgg
     2821 gctcttcctg gccgcgctca cggtccactt ccgggacgtg cgggacctcc tccagacggt
     2881 gctctccgtg ctcttcttcg cgacaccgat cctctacacc ctgaacgacc tcccgaagac
     2941 gaagctgacc cgcatcctcg cgctgaaccc gttcgcaccg cttttcgccg gctggcatga
     3001 cgccctcttc tacggacggg tcagccctct ccagacgtgg gccgcggcgg cggcgatctc
     3061 cgtcgcggcg ttcgccgtcg ggttcgtctt cttcgaccgg ctgcgcgact cgttcccgga
     3121 ggcggtctga gcgcgcgcgc ccgatccgcg gtcgaggcgc gcgacctcgc gaaggtctac
     3181 cggcgctggg gccgcacgcg cgccttcggg acgctgaagt cggcgctcct cggccgcggc
     3241 ctcggccgcg cgctggagcc ctcggagacg gtcgcggcgc tctccggcgt ctccttcgac
     3301 gtccgccgcg gggagacgtt cggcgtcatc ggagcgaacg gctcggggaa gtcgacgctg
     3361 ctcaagctgg tcgccgggct cttcaagccg acctccggga cgctccgcgt cgacggcaag
     3421 gtctcggccc tcatcgagct cggcgccggc ttccaccccg agatctcggg gcgcgagaac
     3481 gtcgtgatca acggggtcat gctcgggctg acgcggaagg agatcgagaa gaagctcccc
     3541 gcgatcgtcg agttctcggg cctctcggag ttcatcgacg agcccgtgaa gacctactcg
     3601 tcggggatgt acgtgaggct cggcttcgcg gtcgccgtcc acgtcgaccc ggaggtcctc
     3661 gtcgtcgacg aggtcctggc cgtcggggac gaggcgttct cgcggcgctg cctcgacacg
     3721 atcaaggagt tctcgacgcg cgggaagacg atcttcttcg tctcgcactc cctcgccctc
     3781 gtcgaggagc tgtgcgatcg cgtcctctac ctctcggggg gccgggtcgg cgggcttggc
     3841 gagccgaggg cgatgctcgc ggcctaccgg ctcgacgtcg cggcggggga agggacgcgc
     3901 cttctcgccg agcacgccgt cgggcaggat catttcgacg ccggtcacgg tggtcagcgg
     3961 cctggccgca gcggcccggc gcggcatcct gatcaagggc ggcgtgttcc tcgaacaggc
     4021 gcgcggcctg c
//
LOCUS       DAOTEZ010000403         3995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_16662_length_3995_cov_6.844670, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000403 DAOTEZ010000000
VERSION     DAOTEZ010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16662_length_3995_cov_6.844670"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..1203)
                     /locus_tag="P5164_16730"
     CDS             complement(31..1203)
                     /locus_tag="P5164_16730"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRY45582.1"
                     /translation="MDADVVVVGAGVVGLASAAELSRAGLSVVVAERHTGPGRETSSR
                     NSQVLHAGLYYPPGSRKARLCVAGNRSLAAFCEAHGVPFQRTGKWLLALEPGEEERLA
                     EIVATGRANGAPLEEVPLARFREEEPHVRAAAAVLSPSTGILDVHGLFRVFRAIAEEN
                     GAAFAFRHELRAAAPAPGGYELVFDDPSGDEVRLSTPRVVNAAGLDADVVAALPGLDV
                     DAAGYRQTWTKGSYFRVRSSRRHLARRLLYPVMPHGFGSVLGIHLTVDLDGELRLGPD
                     VEPLEERRADYAVDESRLGAFLAAARRYLPALVEDDLAPDQSGIRARLQAIGAPFRDF
                     VVAEESERGLAGWVNLVGIESPGLTCCLELAREVRALLAPASEGRTVAAGPTAVRA"
     gene            1290..2546
                     /locus_tag="P5164_16735"
     CDS             1290..2546
                     /locus_tag="P5164_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY45583.1"
                     /translation="MSPTSPDAPGAVTARLAARGLAVLTLVNLFNYLDRFVVASLVES
                     LRNSELRLTDTQAGALMTGFIVVYMVASPFFGVLGDRGSRPRLLALGVFVWSLATAAA
                     GLARSFASLFAARAAVGIGEAAYGTIAPSLLADYFPREKRGRVYAIFFCAIPIGSALG
                     YVVGGLADTHLGWRAAFYVAGLPGLLLALLALTLPDPPRGVTDAGEAPPPGTPKESGW
                     ASYLALARNRPFRIAVLGYAAYTFGLGAMAFWMPAFLERVRGVPKETATIRFGLVVVV
                     TGFVGTFAGGFLGDALLKRTKEAYLWVSGVATLLAVPFAVVALTAAAPGVYFPALVVA
                     QLLVFASTGPINSAIVNDVLPWQRAAAVAACNFTIHVLGDVPSPPLLGAVSDASTLAN
                     AVLLVPAAFLVGGAIWTWGALSGRRA"
     gene            2543..3853
                     /locus_tag="P5164_16740"
     CDS             2543..3853
                     /locus_tag="P5164_16740"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRY45584.1"
                     /translation="MIRTLATAVSRLVLRVFYRNVEAAGLDRVPAAGPTILVLNHPNG
                     LMDPLLLLCLSPRPVSFLAKSPLFTMPFVSLFVKAFDSIPVYRPQDADADVRRNRETF
                     AKAREMLKRGGILALFPEGVSHDEPRLMPLKSGAARIALGAAAEGGVTIVPAGLTYEA
                     RDLFRSSALLRVAEPFTVEPIPLDEKGEPPRTAVKALTERLRVALEGATLQADDAATL
                     ELAAFAESLLPAGDGSLEARVAVRRLLLERAALLRDRAPERLASLVARLERFRAVLSS
                     ARLAPGALDSGTSRAELAASTLAALLVALFLAPPALLGLLAHAPAYNLVGPLAKRLSR
                     GNTDIVATIKVFGAFLFFPLTWIALGTAAGLAWGLPLGLLTGLALPVLGYLALLLLER
                     WEATVAALRALGLSLLRRPALARLRAERDALVAEMAALDADCRV"
BASE COUNT          448 a         1634 c         1332 g          581 t
ORIGIN      
        1 cggcgccgcc cggcgggcgg cgggccgccg tcacgccctg acggctgtcg gcccggccgc
       61 gaccgtccgg ccttccgaag ccggggccag gagcgcccgc acctcgcgcg cgagctcgag
      121 gcagcaggtg agcccgggcg actcgatgcc gacgaggttc acccagccgg ccagcccgcg
      181 ctccgactcc tcggcgacga cgaagtcgcg gaacggcgcg ccgatcgcct ggaggcgcgc
      241 gcggatgccg gactggtcgg gcgcgaggtc gtcctcgacg agcgcgggaa ggtaacggcg
      301 ggccgcggcg aggaaggccc cgagccgcga ctcgtcgacc gcgtagtccg cgcggcgttc
      361 ctcgagcggc tcgacgtcgg gtccgagcct cagctccccg tcgaggtcga cggtgaggtg
      421 gatgcccagg acgctcccga atccgtgcgg catcaccggg tagaggaggc ggcgggcgag
      481 gtggcggcgg gaggatcgga cgcggaagta gctccctttc gtccaggtct ggcggtagcc
      541 cgcggcgtcg acgtcgagcc ccggcagcgc ggcgacgacg tccgcgtcga gtccggcggc
      601 gttcacgacg cgcggcgtcg agaggcggac ctcgtcgccc gacgggtcgt cgaagacgag
      661 ctcgtacccg cccggagcgg gcgccgcggc ccggagctcg tggcggaagg cgaacgccgc
      721 gccgttctcc tccgcgatcg cccggaacac ccggaagagg ccgtggacgt ccaggatccc
      781 ggtcgagggc gagaggacgg ccgccgccgc gcggacgtgc ggctcctcct ctcggaaacg
      841 cgcgagcggc acctcctcga ggggagcgcc gttcgcccgg cccgtggcga cgatctccgc
      901 gaggcgctcc tcctcacccg gctcgagcgc gaggagccac ttccccgtcc gctgaaacgg
      961 cacgccgtgc gcctcgcaga aggccgcgag gctccggttc ccggcgacgc agagccgcgc
     1021 cttccgcgac ccgggcgggt agtagagccc cgcgtggagg acctggctgt tgcgggagct
     1081 cgtttcgcgc ccgggtcccg tgtggcgctc ggccacgacg accgagagcc ccgcgcgcga
     1141 aagctccgct gcagaggcga ggccgaccac cccggcgccg acgacgacga cgtccgcgtc
     1201 cacctccccg atcgtctcac cccttccgcg ccccgtcccg cacggaagag cagtccgcgc
     1261 cgggatcgtg taaaacgtcg ccgccccgca tgagcccgac ctctccggac gcccccggcg
     1321 ccgtgaccgc ccgcctcgcc gcgcggggcc tcgccgtcct cacgctcgtc aacctcttca
     1381 actacctcga ccggttcgtc gtcgcctccc tcgtcgagag cctgcggaat tccgagctgc
     1441 ggctgaccga cacgcaggcc ggcgcgctga tgaccggctt catcgtcgtc tacatggtgg
     1501 cctcgccgtt cttcggcgtc ctcggcgacc gcggctcccg cccgcgcctc ctcgcgctcg
     1561 gcgtcttcgt ctggagcctc gccaccgcgg ccgcgggcct cgcgcggagc ttcgcctcgc
     1621 tcttcgcggc gcgggcggcc gtcggcatcg gcgaggcggc ctacggcacg atcgccccga
     1681 gcctcctcgc cgactacttc ccgagggaga agcgggggcg cgtctacgcg atcttcttct
     1741 gcgcgatccc gatcggctcc gcgctcggct acgtcgtcgg cggcctcgcc gacacgcacc
     1801 tcggctggcg cgccgcgttc tacgtcgccg gcctccccgg cctcctcctc gcgctcctcg
     1861 cgctgacgct ccccgacccg ccgcgcggcg tgacggacgc cggagaggcg ccgcccccgg
     1921 ggaccccgaa ggaatccggc tgggcctcgt acctcgccct cgcgaggaac cgccccttcc
     1981 ggatcgccgt cctcggctac gccgcctaca cgttcggcct cggcgcgatg gcgttctgga
     2041 tgcccgcgtt cctcgagcgg gtccgcggcg ttccgaagga gacggccacc atccgcttcg
     2101 gcctcgtcgt cgtcgtcacc ggcttcgtcg gcacgttcgc cggaggcttc ctcggcgacg
     2161 ccctcctgaa acggacgaag gaggcgtacc tctgggtctc cggcgtcgcc acgctcctcg
     2221 ccgtcccgtt cgccgtcgtc gcgctgacgg ccgcggcgcc gggcgtctac ttcccggcgc
     2281 tcgtcgtcgc ccagctcctc gtcttcgcct ccacggggcc gatcaactcc gcgatcgtca
     2341 acgacgtcct cccgtggcag cgggcggcgg cggtcgcggc gtgcaacttc acgatccacg
     2401 tcctcggcga cgtcccgtcc cccccgctcc tgggcgccgt ctccgacgcc tccaccctcg
     2461 cgaacgccgt cctcctcgtc ccggccgcgt tcctcgtcgg gggcgcgatc tggacctggg
     2521 gcgccctctc ggggcggcgc gcgtgatccg cacgctcgcg accgccgtct cgcgcctcgt
     2581 cctgcgcgtc ttctaccgaa acgtcgaggc ggccggcctc gaccgggtcc ccgccgcggg
     2641 gccgacgatc ctcgtcctga accacccgaa cggcctcatg gacccgctcc tcctcctctg
     2701 cctctcgccg cgtcccgtct cgttcctcgc gaagtcgccg ctcttcacga tgccgttcgt
     2761 ctcccttttc gtgaaggcgt tcgactcgat ccccgtctac aggccgcagg acgccgacgc
     2821 cgacgtgcgg cgcaaccgcg agaccttcgc gaaggcgcgc gagatgctga agcggggcgg
     2881 aatcctcgcc ctcttccccg aaggcgtctc gcacgacgag ccgcgcctca tgccgctcaa
     2941 gtcgggcgcc gcgcggatcg ccctcggcgc cgcggccgag gggggcgtga ccatcgtccc
     3001 cgccggcctc acctacgagg cgcgcgacct cttccggagc tcggccctcc tgcgggtcgc
     3061 cgagcccttc accgtcgagc cgatcccgct cgacgagaag ggggagccgc cgcgaaccgc
     3121 cgtgaaggcg ctgacggagc gcctccgcgt cgcgctggag ggggccacgc tccaggccga
     3181 cgacgcggcg acgctcgagc tggcggcctt cgccgagtcg ctccttccgg ccggggacgg
     3241 cagcctcgag gcgcgcgtcg ccgtccgccg tctcctcctc gagcgggccg cgctccttcg
     3301 cgaccgcgcg ccggagcggc tcgcgtccct cgtcgcccgc ctcgagaggt tccgcgccgt
     3361 cctctcctcc gcgcgcctcg cgcccggagc gcttgattcc ggcacctcgc gcgccgagct
     3421 cgccgcctcc acgctcgccg cgctcctcgt cgctctcttc ctcgccccgc cggcccttct
     3481 cgggctcctc gcccacgctc cggcctacaa cctcgtcggt ccgctcgcga agcgcctctc
     3541 ccgcgggaac accgacatcg tcgcgacgat caaggtcttc ggcgcgttcc tcttcttccc
     3601 cctcacgtgg atcgcgctcg gaacggcggc cgggctcgcc tggggcctcc cgctcggcct
     3661 cctcaccggc ctcgcgctcc ccgtgctcgg ctacctcgcg ctcctcctcc tggagcgatg
     3721 ggaggcgacg gtggccgccc tgcgcgcgct cggcctctcc cttctccgcc gcccggccct
     3781 cgcgcgcctc cgcgccgagc gcgacgcgct cgtcgccgag atggccgccc tcgacgcaga
     3841 ctgtagagtg tagaccagac cccacctacg aaggggtcag atctaccatc tacagtctac
     3901 ggggatcagg cgagggcgag gagggcctcc tccacggcgc cccgaacgag cgggactttg
     3961 tagtcgtttt ccgacatcgg ctcggccccc ttcac
//
LOCUS       DAOTEZ010000404         3993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_16677_length_3993_cov_5.372016, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000404 DAOTEZ010000000
VERSION     DAOTEZ010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16677_length_3993_cov_5.372016"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="P5164_16745"
     CDS             <1..1058
                     /locus_tag="P5164_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020600764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY45585.1"
                     /translation="LRQSILFILLWLIFRKALSPGELIAFQFILNTIFGPLQQMGNVL
                     LNHREAEASLAAFGHLMATPVEERPEEPVDVGEIESLRFEDVSFRYRGAAEDALDHIG
                     FEARLGDTIAFVGPSGSGKSTLVKLLVGLYRPTAGVVTIDGVPINELRYNRVRRQIGF
                     VTQDPQLFSGTIRENLQLVKEDATDEEMLEALRRASATPLLTRSGQGLDTRVGESGLR
                     VSGGERQRLSIARALLRRPRLLIFDEATSALDSITEQEIAASVREVSGSRQHMVILIA
                     HRLSTIAHAGTIHVLERGRIVETGSHDELVERRGLYYAMWRQQIGEREPWGAPPRAAA
                     PPLDEAAIAEDALVAEA"
     gene            complement(1105..2358)
                     /locus_tag="P5164_16750"
     CDS             complement(1105..2358)
                     /locus_tag="P5164_16750"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HRY45586.1"
                     /translation="MTEQTRIVSFRVDADGRLVADPSDSRQLGLRPGDALRIETGDPL
                     WVARRPVSNLARVYVEPTNACNLTCSTCVRNVWSAESGFMTEETFDALLRGLAALPRP
                     PVLFFGGFGEPLAHPRILPMLEEARSAGAEVELITNGILLGDEVRRALVSLGLRRLWV
                     SIDGATPAAYRDVRIGDALPEILANLAAFRALREASPGRSTRLGLVFVAMRRNLGQLP
                     DVVKLGRAAGMDRLLVTNVLAYTDDLKDETLYDEGLAPGDGDSLERTAILLPRLEPSG
                     AVREALAAVVDGGPVDLRPRLSAVRGRNFCPFVERGTTSVRWDGTVSPCLALLYDHDY
                     WLGDRRRHSHASAVGNVRDRSLADVWADPAYVELRRRAQAFDFSPCSSCNTCEMADDN
                     SRDCSGSPLPACGGCLWAQGLIQCP"
     gene            complement(2400..2828)
                     /locus_tag="P5164_16755"
     CDS             complement(2400..2828)
                     /locus_tag="P5164_16755"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015402800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HRY45587.1"
                     /translation="MRKKVLVLCTGNSCRSQMGEGWLRHDLGESVEAFSAGTTPSFVH
                     PRAIQVMAEAGVDLSTHSSKGVESLRGVAFDLVVTVCDSAREACPVFPGAKRTVHHAF
                     EDPAHAGTGNEALPAFRRVRDEIRSVLVPLVARELGLPGR"
     gene            complement(2837..3922)
                     /gene="arsB"
                     /locus_tag="P5164_16760"
     CDS             complement(2837..3922)
                     /gene="arsB"
                     /locus_tag="P5164_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HRY45588.1"
                     /translation="MSEAAAPKRLNVFERWLTLWVALCMVAGVALGKLFPGATDAVRK
                     LEFGEGSQINVAIAVLIWLMVYPMMLKIDFTSLAGVGRRPKGLLVTLFVNWLVKPFSM
                     AFLAWLFFKNVFAAWIPAGLADQYIAGAIILAAAPCTAMVFVWSYLTDGDPAYTLVQV
                     SVNDLIMLVLFAPIVTFLVAGASNLTVPFKVLLLSVLVFIVIPLALGSLSRTLLIRAR
                     GRAWFEGTFLPVFHPVTVVALLATLVLIFAFQADNLLTNWFHVVLIAVPLLIQVYFNS
                     GLAYGLMKLLKVPHAVAAPGALIGASNFFELAVATAIALYGPGSGAALATVVGVLVEV
                     PVMLSVCSFCNRTRGWFPAPAATAPSS"
BASE COUNT          630 a         1357 c         1501 g          505 t
ORIGIN      
        1 tcctccggca gtcgatcctc ttcatcctcc tctggctcat cttccgcaag gcgctctcgc
       61 cgggcgagct gatcgcgttc cagttcatcc tgaacacgat cttcgggccg ctgcagcaga
      121 tggggaacgt ccttctgaac caccgggagg ccgaggcctc gctcgcggcg ttcgggcacc
      181 tgatggcgac gccggtcgag gagcgtccgg aggagccggt cgacgtcggc gagatcgaga
      241 gcctccgctt cgaggacgtc tccttccgct atcgcggggc cgcggaggac gccctcgacc
      301 acatcgggtt cgaggcgagg ctcggcgaca cgatcgcgtt cgtcggcccg tcgggctccg
      361 ggaagtcgac gctcgtcaag ctcctcgtcg ggctctaccg gccgaccgcg ggggtcgtca
      421 cgatcgacgg cgtgccgatc aacgagctgc gctacaaccg cgtgcggcgg cagatcggct
      481 tcgtcaccca ggacccgcag ctcttctcgg ggacgatccg cgagaacctc cagctcgtga
      541 aagaggacgc gaccgacgag gagatgctcg aggccctccg gcgcgcgtcg gcgacgccgc
      601 tcctgacgcg ctcgggccag gggctcgaca cgcgcgtcgg ggagtcgggc ctccgggtct
      661 ccggcgggga gcgccagcgc ctctcgatcg cccgcgcgct cctgcgccgc ccccgcctcc
      721 tcatcttcga cgaagcgacg tcggcgctcg actcgatcac cgagcaggag atcgccgcgt
      781 cggtccgcga ggtctcgggg agccggcagc acatggtgat cctgatcgcg caccgcctca
      841 gcacgatcgc gcacgccggg acgatccacg tcctcgagcg cgggcggatc gtcgagacgg
      901 ggagccacga cgagctcgtc gagaggcggg gcctctacta cgcgatgtgg cgccagcaga
      961 tcggcgaacg cgagccgtgg ggggcgccgc ctcgcgcggc ggcgccccct ctcgacgagg
     1021 cggcgatcgc cgaggacgcg ctcgtcgcgg aggcctgagg gcgggccgag gcggggcgga
     1081 cggaggcgcc gcgtccgggg agcgtcacgg gcactggatc agcccctgcg cccagaggca
     1141 gccgccgcag gcggggaggg gagagcccga gcagtcgcgg gagttgtcgt cggccatctc
     1201 gcacgtgttg caggagctgc acggcgagaa gtcgaacgcc tgggcgcgcc gccggagctc
     1261 gacgtacgcg gggtcggccc agacgtcggc gaggctcctg tcccggacgt tcccgacggc
     1321 gctggcgtgc gagtgccgcc ggcggtctcc gagccagtag tcgtggtcgt agaggagggc
     1381 gaggcagggg ctgacggtcc cgtcccacct cacggaagtc gtcccccttt cgacgaaggg
     1441 gcagaagttg cgaccgcgga cggcggagag gcgagggcgc aggtcgaccg gtccgccgtc
     1501 gaccacggcg gcaagggcct cgcggacggc acccgacggc tcgaggcgcg ggaggaggat
     1561 cgccgtcctc tccagggagt ccccgtcgcc cggggcgagc ccctcgtcgt agagcgtctc
     1621 gtccttcagg tcgtcggtgt acgcgaggac gttcgtcacg aggaggcggt ccattcccgc
     1681 cgcgcgtccc agcttcacga cgtcggggag ctgcccgagg ttccggcgca tcgcgacgaa
     1741 gacgaggccg agccgggtgc tccggccggg cgaggcctcg cgcagcgccc ggaacgcggc
     1801 gaggttcgcc aggatctccg ggagggcgtc gccgatccga acgtcgcgat acgcggcggg
     1861 cgtcgccccg tcgatcgaga cccagagccg gcgaagcccg agcgagacga gggcgcgacg
     1921 gacctcgtcg ccgaggagga tgccgttcgt gatgagctcg acctcggcgc cggcgctgcg
     1981 ggcctcctcc agcatcggga ggatccgggg gtgcgcgagc ggctcgccga agccgccgaa
     2041 gaagaggacc gggggacggg ggagcgccgc gagcccgcgg aggagggcgt cgaacgtctc
     2101 ctccgtcatg aagcccgact cggcgctcca gacgttccgc acgcacgtcg agcaggtcag
     2161 gttgcaggcg ttcgtcggct cgacgtagac gcgcgcgagg ttcgagaccg ggcgtcgggc
     2221 gacccagagc gggtcgcccg tctcgatccg gagcgcgtcg cccggccgga ggccgagctg
     2281 ccgggagtcg gacgggtcgg cgacgaggcg gccgtcggcg tcgacgcgga aggagacgat
     2341 tcgcgtctgc tccgtcaccg gcccgcccct tccgcgcggc gtcgcctcat cgcggggcct
     2401 cagcgcccgg gcagccccag ctcccgtgcg acgagcggga cgaggacgga ccggatctcg
     2461 tcccggacac ggcggaacgc ggggagcgcc tcgttcccgg tcccggcgtg ggccgggtcc
     2521 tcgaaggcgt gatggacggt ccgcttcgcg cccgggaaga ccgggcatgc ctcgcgcgcc
     2581 gagtcgcaca cggtgacgac gaggtcgaac gcgaccccgc ggaggctctc gacccccttg
     2641 ctcgagtgag tagagaggtc gacgcccgcc tccgccatga cctggatcgc ccgcgggtgg
     2701 acgaaggacg gcgtcgtgcc ggcggaaaac gcctcgacgc tctcgccgag gtcgtgccga
     2761 agccacccct cgcccatctg cgagcggcag gagttcccgg tgcagaggac gaggaccttc
     2821 ttcctcaccg ggctcctcag gaggagggtg ccgtggcggc gggcgccggg aaccatcccc
     2881 gcgtccggtt gcagaaggag cagaccgaaa gcatgaccgg cacctccacg aggacgccga
     2941 cgacggtcgc gagcgcggcg cccgagcccg ggccgtagag cgcgatcgcc gtcgcgacgg
     3001 cgagctcgaa gaagttgctc gcgccgatga gggcgccggg cgccgcgacg gcgtgcggca
     3061 ccttcaggag cttcatcagg ccgtaggcga ggccggagtt gaagtagacc tggatcagga
     3121 gcgggaccgc gatcaggacg acgtggaacc agttcgtcag gaggttgtcg gcctggaacg
     3181 cgaagatgag gacgagcgtc gcgaggagcg cgacgacggt gacggggtgg aagacgggga
     3241 ggaaggtccc ctcgaaccac gcccgccctc tcgcccgaat caggagcgtc cggctgaggc
     3301 tcccgagggc gagcggaatg acgatgaaga cgaggacgga gaggaggagg accttgaacg
     3361 gcaccgtcag gttgctcgcg ccggcgacga ggaacgtcac gatcggcgcg aagaggacga
     3421 gcatgatcag gtcgttcacc gagacctgga cgagcgtgta ggccgggtcc ccgtccgtca
     3481 ggtacgacca gacgaagacc atcgccgtgc agggcgccgc ggcgaggatg atcgcccccg
     3541 cgatgtactg gtcggcgagg ccggccggga tccaggccgc gaagacgttc ttgaagaaga
     3601 gccaggcgag gaaggccatc gagaacggct tgacgagcca gttcacgaag agcgtgacga
     3661 ggaggccctt cggccgcctc ccgacccccg cgaggctcgt gaagtcgatc ttcagcatca
     3721 tggggtagac catgagccag atcaggacgg cgatcgcgac gttgatctgg ctcccctcgc
     3781 cgaactcgag cttccgcacg gcgtcggtcg ccccggggaa gagcttcccc agggcgaccc
     3841 cggcgaccat gcagagcgcg acccagaggg tcagccagcg ctcgaagacg ttgaggcgct
     3901 tcggggcggc ggcctcgctc actcgaacct cctacgcggc gggcttcacc gcgcggacga
     3961 aggcgctccg gaacttcccg tcgacctccg cgc
//
LOCUS       DAOTEZ010000405         3990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_16696_length_3990_cov_5.730623, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000405 DAOTEZ010000000
VERSION     DAOTEZ010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16696_length_3990_cov_5.730623"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="P5164_16765"
     CDS             <1..258
                     /locus_tag="P5164_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45589.1"
                     /translation="QLGRIGKRLMENHGATFSWDEAVVNEIGSRCKEVESGARNVDSI
                     LTRTVLPDLSGEFLARMAQGESISKVHVRVGEGGAFAYDIA"
     gene            507..2750
                     /gene="tssI"
                     /locus_tag="P5164_16770"
     CDS             507..2750
                     /gene="tssI"
                     /locus_tag="P5164_16770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HRY45590.1"
                     /translation="MGSYVQQNRKIRVTTPIGEDVLLLKSLEGTDELSRPFRYRLEMI
                     AENKAKVPFDGILGKKVTVHLQLPDESTEHHVNGVCTRFSQAGRDEHFTLYRAEIVPE
                     VQLLARKARSRVFQRKSVPEILKEVMKGVTAEWKLQGSYEKRDYCVQYRETDFSFVSR
                     LMEEEGILYFFRQEDGKHTMVLADASSEFPELPGESELVYEELEGGTRDENRVWAWER
                     AQEARSVRYVLWDHCFELPHKHLEAEEPILASVSAGREPHALAVEGSGEWELYDWPGE
                     YAQRFDGINRSGGEQQDELQKIFRDNARTVGLRMDEEAALAVEIRGSSNVKHLAAGHT
                     ITLKRHFSGDGAYVLASVTHRATFGADYRSGKDEELAYANEFTCFPAALPFRPRRRTP
                     KPVVPGSQTAVVVGPKGEEIFTDRYGRVKVQFHWDRDGKHDEDASCWVRVAQLAAGRR
                     WGSSFWPRIGQEVIVDFLEGDPDRPIVVGTVYNAEQMPPYLGQGPDGKHRNDNRVSGI
                     KTSTTTGGNGFNELRFDDAKGAEQLFVHAERDLDVRVKNDARESVLGDRHVTIGHAGE
                     GGGTGEYREHVRKGKHVAVEGGLAARYGGAKVLVGGGDEEGDLDLYVEGEMKTTVDGS
                     ANTHVKGDRVAKVDGNAGVKVMLNAGLLVDGRLSLRANEETHLSSAGKVVITAPEITV
                     GRSDSSFIKIDASGVTILGPQVRINSGGAKDAGWQLDPPEPAEAAKAAPAAPAEADDA
                     KTGQKSS"
     gene            2762..3052
                     /locus_tag="P5164_16775"
     CDS             2762..3052
                     /locus_tag="P5164_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HRY45591.1"
                     /translation="MAMAARLGDMHNCPLTNPDGSPHTGGPIQTSEGSVLIEYMPAAR
                     LGDLATCVGASDAISTGDATCLIGYMPAARVGDATAHGGVIVAGGTSVLIGG"
     gene            complement(3104..>3990)
                     /locus_tag="P5164_16780"
     CDS             complement(3104..>3990)
                     /locus_tag="P5164_16780"
                     /inference="COORDINATES: protein motif:HMM:NF017730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HRY45592.1"
                     /translation="MRTILGNGAFRGLEASWRGVDFLVRRLDVDGPLTLHLVDLSTEE
                     LRADLLAGDDLGRTGLHRLLVEKTIGTPGGQAWAAFALLDSFGPSRQDAEALGRLARI
                     AAKAGTAVLAGAEPALVGCASLAETPDPDGWVDPSGEGADAWEALRRLPEARSVGLAL
                     PRLLLRLPYGKETNAVDAFDFEELASPPEHEQYLWGSPALATALLLGEAFLEDGWSLR
                     PGSAQEISGLPFALVAEDGEKRAVPCAEALFTVRAMQAVAEKGLMPLLTMKGTDTVRL
                     AIFQSIAEPPAPLAGLWG"
BASE COUNT          651 a         1315 c         1531 g          493 t
ORIGIN      
        1 cagctcggcc ggatcggcaa gcgcctcatg gagaaccacg gcgcgacgtt cagctgggac
       61 gaggccgtcg tgaacgagat cggcagccga tgcaaggagg tcgagagcgg ggctcgcaac
      121 gtcgactcga tcctcacgag gaccgtcctc cccgacctct cgggcgagtt cctcgcgcgg
      181 atggcgcagg gagagtcgat ctcgaaggtc cacgtccgcg tcggggaggg cggcgccttc
      241 gcctacgaca tcgcctgacg tccccgcgcg gaagcggttc gagggccggg gccgcgcagg
      301 cagtcccggc ccttcgtccg cccccttggc gcgcgagacg cggtcgggcg gacggcgctg
      361 ctgagaagcc gtcgctctgc cgggccaaga acgtcgcccg ccgtgacgcg cggcacgtcc
      421 tcgcggcgtc gaaggcgtcg cgcgcgagcc gatccgggac catattggag ggaacgggac
      481 gggggcggtg ggcacggggg tatcgcgtgg gcagctacgt ccagcagaac cgcaagatcc
      541 gcgtcacgac cccgatcgga gaggacgtcc tcctcctgaa gtcgctcgag ggtacggacg
      601 agctctcccg gccgttccgc taccggctcg agatgatcgc ggagaacaag gcgaaggtcc
      661 ccttcgacgg catcctcggc aagaaggtga ccgtccacct ccagctgccg gacgagtcga
      721 ccgagcacca cgtcaacggc gtctgcaccc ggttctcgca ggcgggtcgc gacgagcact
      781 tcaccctcta ccgggccgag atcgtccccg aggtccagct cctcgcgcga aaggcgcgga
      841 gccgcgtctt ccagcggaag tctgtgcccg agatcctcaa ggaggtgatg aagggcgtca
      901 ccgccgagtg gaagctccag gggtcgtacg agaagaggga ctactgcgtc cagtaccggg
      961 agaccgactt cagcttcgtg tcccgcctga tggaggagga ggggatcttg tatttcttcc
     1021 ggcaggagga cgggaagcac acgatggtcc tggcggacgc ctcgtccgag tttcccgagc
     1081 tgccgggcga gagcgagctc gtctacgagg agctggaagg gggcacccgg gacgagaacc
     1141 gggtctgggc ctgggagcgg gcgcaggagg cgcggtcggt ccggtacgtc ctgtgggacc
     1201 actgcttcga gctgccgcac aagcacctgg aggcggagga gccgatcctc gcgtccgtct
     1261 ccgccggccg cgagccgcac gcgctcgcgg tggagggctc gggcgagtgg gagctctacg
     1321 actggccggg cgagtacgcg cagcggttcg acgggatcaa ccggtcgggc ggggagcagc
     1381 aggacgagct gcagaagatc ttccgggaca acgcgcggac ggtcggcctc cggatggacg
     1441 aggaggccgc cctggccgtg gagatccgcg gctcgagcaa cgtcaagcac ctcgcggcgg
     1501 gccacacgat cacgctcaag aggcacttct cgggcgacgg agcgtacgtc ctcgcgtcgg
     1561 tgacgcaccg ggcgacgttc ggtgccgact acaggtcggg gaaggacgag gagctcgcct
     1621 acgcgaacga gttcacctgc ttcccggcgg cgctcccttt ccggccgcgg cggcggactc
     1681 cgaagccggt cgtgccggga agccagacgg ccgtcgtcgt cgggccgaag ggggaggaga
     1741 tcttcaccga caggtacggg cgggtgaagg tccagttcca ctgggaccgg gacgggaagc
     1801 acgacgagga cgcgtcctgc tgggtccggg tcgcccagct cgcggcgggg cggcggtggg
     1861 ggtcgtcgtt ctggccgcgg atcggccagg aggtgatcgt cgacttcctc gagggagacc
     1921 cggaccggcc gatcgtcgtc ggaaccgtct acaacgcgga gcagatgccg ccctacctcg
     1981 gacaggggcc ggacggaaag caccggaacg acaaccgggt ctcggggatc aagacgagca
     2041 cgacgacggg gggaaacggc ttcaacgagc tccgcttcga cgacgcgaaa ggggcggagc
     2101 agctcttcgt ccacgccgag cgggacctcg acgtgagggt gaagaacgac gcgcgggaga
     2161 gcgtcctcgg cgaccggcac gtgacgatcg ggcacgcggg ggagggcggg gggacgggcg
     2221 agtaccgcga gcacgtccgg aaggggaagc acgtggcggt cgagggtggc ctggcggcgc
     2281 gctatggcgg ggcgaaggtc ctcgtcggag gcggcgacga agagggggac ctcgacctct
     2341 acgtcgaggg ggagatgaag acgaccgtcg acggctccgc gaacacgcac gtcaaggggg
     2401 accgggtcgc caaggtcgac gggaacgcgg gcgtgaaggt catgttgaac gcggggctcc
     2461 tggtggatgg ccgcctgtcg ctccgggcga acgaggagac tcacctctcg agcgcgggaa
     2521 aggtcgtcat caccgccccg gagatcaccg tcggccgctc ggacagcagc ttcatcaaga
     2581 tcgacgcgag cggcgtgacg atcctcgggc cgcaggtgag gatcaacagc ggcggcgcga
     2641 aggacgcggg ctggcagctc gacccgcccg agcccgcgga ggcggcgaag gccgcgccgg
     2701 ccgcgccggc cgaggcggac gacgcgaaga ctgggcagaa gtcgagctga ggagggcgcc
     2761 gatggcgatg gcggcccggc tgggcgacat gcacaactgt ccgttgacga acccggacgg
     2821 gagcccccac accggcgggc cgatccagac cagcgagggc agcgtgctga tcgagtacat
     2881 gccggcggcg cgcctgggcg acctggcgac ctgcgtcgga gcttcggacg cgatctccac
     2941 gggcgacgcc acctgcctga tcggctacat gccggccgcg cgcgtgggcg acgcgaccgc
     3001 gcacggaggg gtgatcgtgg ccggcggcac gtcggtcctc atcggaggct gaccgccgat
     3061 cccttcgggc cagcgaccga cctccgcgag gacggccggc cgctcagccc cagagcccgg
     3121 ccagcggggc cggcggctcg gcgatcgact ggaagatcgc gagcctcacg gtgtcggtcc
     3181 ccttcatcgt caggagcggc atcagcccct tctccgcgac ggcctgcatc gcccggacgg
     3241 tgaagagcgc ctcggcgcac ggcacggcgc gcttctcgcc gtcctccgcc acgagggcga
     3301 acgggaggcc ggagatctcc tgcgccgacc cggggcggag gctccagccg tcctcgagga
     3361 acgcctctcc gagaaggagg gccgtcgcga gcgccgggct tccccagagg tactgctcgt
     3421 gctcgggcgg cgaggcgagc tcctcgaagt cgaacgcgtc gacggcgttc gtctccttcc
     3481 cgtaagggag cctgagcagg aggcgcggca gcgcgaggcc gacggagcgc gcctcgggga
     3541 gccgtcggag cgcctcccag gcgtcggcgc cctcgccgga ggggtcgacc cagccgtccg
     3601 ggtcgggcgt ctcggcgagc gaggcgcagc cgacgagtgc cggctcggct ccggcgagga
     3661 cggccgtccc cgccttcgcc gcgatccgag cgaggcgccc gagcgcctcg gcgtcctgac
     3721 gcgacggccc gaacgaatcg aggagggcga aggcggccca cgcctgcccg ccgggcgtgc
     3781 cgatggtctt ctcgaccagg aggcggtgga gaccggtccg cccgaggtcg tctcccgcga
     3841 ggaggtcggc gcggagctcc tccgtcgaga ggtcgacgag gtggagcgtg agcgggccgt
     3901 cgacgtcgag ccgccggacg aggaagtcga cgccgcgcca gctcgcctcc agtcctcgga
     3961 acgcgccgtt cccgaggatc gtccgcatca
//
LOCUS       DAOTEZ010000406         3984 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_16736_length_3984_cov_5.771952, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000406 DAOTEZ010000000
VERSION     DAOTEZ010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16736_length_3984_cov_5.771952"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..666)
                     /locus_tag="P5164_16785"
     CDS             complement(88..666)
                     /locus_tag="P5164_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRY45593.1"
                     /translation="MTRPDPVVALAEEYPRFLAFLAKRTGDRAVAEEILQEAFARAVT
                     KAHGLREDSSAVAWFYQVLRNALVDHHRRSASEGRAMAAFAAEQEGEARDEELEREVC
                     RCVGRIAEDLKPEYAEVLRGVEVEGLSLKDFAGKAGITPNNAAVRVHRAREALRRRLV
                     ATCSTCAAKGCTDCTCQSHERREESEGHAGRS"
     gene            complement(692..1135)
                     /locus_tag="P5164_16790"
     CDS             complement(692..1135)
                     /locus_tag="P5164_16790"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HRY45594.1"
                     /translation="MSFTDSIRNFLGTGPGARPAEAGPSCCSGGDACERPSSEGSKAP
                     ASTAAGAASPAGSLPAPAQRLEVTVEKGYRPARLTARAGSPLRVTFLRNETSGCSKEV
                     VFPGLGIRRALPTGERVSIDLPPLAAGIVPFTCGMGMMKGGIDVR"
     gene            1270..2670
                     /locus_tag="P5164_16795"
     CDS             1270..2670
                     /locus_tag="P5164_16795"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRY45595.1"
                     /translation="MLRVPRRTLLSLFLPILLASSGSASLAEELPTAGGAGRLGAPEP
                     SGKAARRATVDPLTPESAVRLSLLADGSLRSRLLEVEASAGRLAEARRLPDPELEVEL
                     RRPSREEEGAGPRVEASLGWDLSAVLGRRPLVAAARADLDAARARADAALARHALEVE
                     SAFHGLVAARRKLALARARTELAELGADAARAVADAGGAALLSPLREGARREAATLAL
                     AEAGLSVVEAEERLVALLGGGAVPGVHDAVAGVGAEAAPPAPQAPELSGLEESAVAAS
                     LELAELRARLGAAEARARLARADRHRPGLEIRLLAEREDGAWSAGAGLSLRLPVFDRG
                     RGRVAALEAEVRSIRALLGGAEVLVRSSARVTAARLSAAHARAARLTGPVRAFHERMT
                     SETLLQANAMQVGIPALLSARDSALRAEADAADALRDLAVADAAARALRAGVRPDAPA
                     TRPDSPTGIALAEGDH"
     gene            2670..3965
                     /locus_tag="P5164_16800"
     CDS             2670..3965
                     /locus_tag="P5164_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HRY45596.1"
                     /translation="MDRRSLLRFAGLAALGSALPLSADGPNAASRPFGARRGPTRPSR
                     SGVGSVVTPNGSTLPLHRSGHVLVGHLVAAETEHEFAPGLTALVWGYNGGTPGPTIEA
                     TEGDRLRIYVTNRLPEPTTVHWHGLVLPNGMDGVSGLNQAPIPVGETWVYELDLHRPG
                     TFMYHSHYDEMTQVALGLVGMIVVHPRVPKGPPVDRDFVLMTHEWSLPVGARRPDPGA
                     MSDFNLLTFNGKAYPGTAPLVVGTGERVRIRIGNLSPMDHHPIHLHGLSFEVTGTDGG
                     DVPPSARWPETTVLVPTGSARVIELVPTEPGDWAVHCHMTHHVMTQMGHGLPNVLGAD
                     LASVDERLSRLVPGAMVMGTTGMGGMGEMEMPVPPNSLPMKGAHGPFGTIDRGGMFTV
                     LKVRDDPASADTDGWFRHSEGTVAGPADAARLAEDGIVP"
BASE COUNT          479 a         1583 c         1392 g          530 t
ORIGIN      
        1 ccgacggagg aggagcggga cgcgatccgc gggctcatga aggggggctc cgcgacccgg
       61 gccgcgtagc cggggcctgc gggcgcttca gctgcgtccc gcgtgtccct cgctttcttc
      121 gcgccgttcg tggctctggc aggtgcagtc ggtgcagccc ttcgccgcgc aggtcgagca
      181 ggtcgcgacg agccgccttc ggagcgcctc gcgcgcccgg tggacgcgga ccgccgcgtt
      241 gttcggcgtg attccggcct tcccggcgaa gtccttcagg gaaagccctt cgacctcgac
      301 gccccgcaac acctcggcgt actcgggctt caggtcttcg gcgatccgcc cgacgcagcg
      361 acacacctct cgttccagct cctcgtccct cgcctcccct tcctgctccg cggcgaacgc
      421 agccatcgcg cgcccctcgg acgcgctccg ccgatggtgg tcgacgagag cgttgcggag
      481 gacctggtag aaccaggcca cggcggagga gtcctcgcgc aggccgtgcg ccttcgtcac
      541 ggcccgcgcg aatgcctcct ggaggatctc ctcggccacg gcccggtccc ccgtccgctt
      601 cgcgaggaac gcgaggaaac gcgggtactc ttccgcgagg gcgacgaccg ggtcggggcg
      661 ggtcacccgc gcattctgac aaggtccgtc gtcagcgcac gtcgatcccc cccttcatca
      721 tccccatccc gcaggtgaac gggacgattc cggcggccag cggggggagg tcgatcgaga
      781 ccctctcccc cgtcgggagc gcccggcgaa tgccgagccc gggaaagacg acctccttcg
      841 agcagccgga ggtctcgttc cggaggaacg tgacgcgcag gggagagccg gcgcgggccg
      901 tgagcctcgc gggacggtag cctttctcca cggtcacctc gagccgctgc gccggcgcgg
      961 ggagggagcc tgcgggcgac gcagctcccg cggctgtgga cgcgggggct ttcgatccct
     1021 cggaggacgg gcgctcgcag gcgtcgccgc cgctgcagca ggaaggcccc gcttcggccg
     1081 gccgggcgcc gggtcccgtg cccaggaaat tgcggatgct gtcggtgaag ctcatgtcgg
     1141 tctcctttcg tcggtcgcaa gaggagacga cgggcctcgc cggaccttac agggcctccc
     1201 ccgttcccca cgccggcccc ctgtaaggcc ccgggcgacc ccgcgtctcc gctcccggag
     1261 gccgacgaca tgcttcgagt cccgcgacgg acgctcctct cgctcttcct tccgatcctc
     1321 ctcgcgagct ccggctccgc atcgctcgcc gaggaactcc cgactgcggg cggagccggg
     1381 cgcctcggcg cgcccgagcc ctccggcaag gccgctcgac gggccacggt cgatcccctc
     1441 acgcccgaga gcgccgtccg gctctcgctg ctcgccgacg ggtcgctccg ttcgcgcctc
     1501 ctcgaggtgg aggcgtcggc cgggaggctc gccgaggcgc ggcgcctccc ggatcccgag
     1561 ctcgaagtcg agctccggcg gccttcgcgg gaggaggagg gcgcggggcc ccgcgtcgag
     1621 gcctctctcg gctgggacct gtcggcggtc ctcggcaggc gcccgctcgt cgccgccgcg
     1681 cgggccgatc tcgacgcggc ccgcgcgcgc gcggacgccg cgctcgcccg gcacgccctc
     1741 gaggtcgaga gcgcgttcca cggactggtc gctgcgcggc ggaagctcgc gctcgcccgg
     1801 gcccgcaccg agctggccga gctcggcgcc gacgccgccc gggccgtcgc cgacgcagga
     1861 ggcgcggcgc tcctctcgcc gctccgggaa ggggcccgac gcgaggccgc cacgctcgct
     1921 ctcgccgagg ccggcctctc ggtcgtcgag gccgaggagc ggctcgtcgc cctcctcggc
     1981 ggcggcgccg tcccaggggt ccacgacgcc gtcgccggcg tcggagccga agcggccccc
     2041 cccgcaccgc aggctccgga actctccggc ctcgaggaat cggccgtggc ggcgagcctc
     2101 gagctcgcag agcttcgggc gcgcctcggc gctgcggagg cgcgagcccg cctcgccagg
     2161 gccgaccgcc acagaccggg cctcgagatc cgcctcctcg ccgagcggga ggacggcgcc
     2221 tggagcgccg gggcgggcct ctcgctccgc ctccccgtct tcgaccgcgg ccgcgggcgg
     2281 gtcgccgcgc tcgaggccga ggtccggtcg atccgcgcgc tcctcggcgg cgccgaggtc
     2341 ctcgtccgtt cctccgcccg cgtcacggcg gcccgtctct ccgccgccca cgcgcgcgcc
     2401 gcgcggctca ccggcccggt ccgcgccttc cacgagcgga tgacgtcgga gacgctcctc
     2461 caggccaacg ccatgcaggt ggggatcccc gcgcttctct ccgctcgcga ctcggccctc
     2521 cgtgcggaag cggacgccgc ggacgccctg cgagacctcg cggtcgccga cgccgcggcg
     2581 cgcgccctgc gcgcgggcgt ccgccccgac gctcccgcga cccgccccga ttccccgacc
     2641 gggatcgcac tcgccgaagg agatcactga tggaccgtcg ctctctcctc cgcttcgccg
     2701 gcctcgccgc cctcggcagc gccctgcctc tctccgcgga cgggccgaac gcggcctccc
     2761 gtcccttcgg ggcacggcgc ggcccgaccc ggccctcccg gtccggcgtc ggctccgtcg
     2821 tcactccgaa cggctcgacg ctgccgctcc accgctccgg ccacgtcctc gtcggccacc
     2881 tcgtggccgc ggagaccgag cacgaattcg cgccgggcct gaccgccctc gtctggggct
     2941 acaacggcgg cacaccgggg ccgacgatcg aggcgaccga gggggaccgc ctccggatct
     3001 acgtcacgaa ccggctcccc gagccgacga ccgtccactg gcacggcctc gtcctgccga
     3061 acggaatgga cggcgtctcg ggcctcaacc aggcgccgat cccggtcggc gaaacgtggg
     3121 tctacgagct cgacctccac cgccccggga ccttcatgta ccactcgcac tacgacgaga
     3181 tgacgcaggt cgccctcggc ctcgtcggga tgatcgtcgt ccacccgcgc gtcccgaagg
     3241 gcccgccggt cgacagggac ttcgtcctga tgacgcacga gtggagcctc ccggtcggcg
     3301 cgcgccgccc cgacccgggc gcgatgagcg acttcaacct cctgacgttc aacgggaagg
     3361 cgtaccccgg gacggcgccg ctcgtcgtcg gtaccgggga acgggtccgc atccggatcg
     3421 ggaacctctc gccgatggac caccacccga tccacctcca cggcctctcg ttcgaggtga
     3481 cggggaccga cggcggcgac gttcccccgt ccgcacgctg gcccgagacg acggtcctcg
     3541 tcccgaccgg ctcggcgcgg gtcatcgagc tcgtcccgac cgagccgggc gactgggccg
     3601 ttcactgcca catgacgcac cacgtcatga cgcagatggg gcacggcctc ccgaacgtcc
     3661 tcggcgccga cctcgcgtcc gtcgacgagc gcctctcgcg cctcgtcccg ggggcgatgg
     3721 tcatggggac gaccggcatg ggtgggatgg gcgagatgga gatgccggtt cccccgaaca
     3781 gcctcccgat gaagggggcg cacgggccgt tcggcaccat cgacaggggc gggatgttca
     3841 ccgtcctgaa ggtgcgcgac gacccggcgt ccgccgacac cgacgggtgg ttccgccact
     3901 ccgaggggac cgtggccggg cccgccgacg ccgcccgcct cgcggaggac gggatcgttc
     3961 cctgaagctc gggaggatcg gatg
//
LOCUS       DAOTEZ010000407         3981 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_16757_length_3981_cov_5.909068, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000407 DAOTEZ010000000
VERSION     DAOTEZ010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16757_length_3981_cov_5.909068"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="P5164_16805"
     CDS             <1..279
                     /locus_tag="P5164_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRY45597.1"
                     /translation="ALGASRAAVDAGWIGHEHQVGQTGKVVSPTLYIACGISGAIQHM
                     AGMGSSKVIVAINKDADAPIFKAATYGIVGDLFQIVPAMTEAVKKAKA"
     gene            complement(459..2279)
                     /locus_tag="P5164_16810"
     CDS             complement(459..2279)
                     /locus_tag="P5164_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45598.1"
                     /translation="MRKTSSFLVLTVLLGLAALTPAVLHGALAAPQGTELWGGPGAKG
                     TGNQSARFDTVVYVTGPAPAVGAVEFLAGGKVLATVPFTFAANGVALVPAPDEVAEKG
                     AFLYRVRSDVPVSAWSETYNEASSGRYGVSLGAAAPSELLGPGDEATGGGAEASSETG
                     AGHARTNAGVVCVDTASQPCRLEVALFSSGALVGSGTIETAPGSAAQASLATLVPAAV
                     GKAGLALRLRLLSGQGYPYAIRNDNQTSDGTAIPLAVKRNAFSTAPTIASFTASPATG
                     CAPLETTFAWETSGAAKVSISGVGNDLPATGSVKRTVHATGDFILTATSSTGQSTVRP
                     LRITITPSTPPPTPAPASATLPTSGVVTGILPVGTGPVTVEFVSRESTGSTFTVQGTS
                     FIYTAGTSPGTDVVKITATGSCGGASAEFTAHVVLPGAPRILSFEAIPERGCAPSTNF
                     LLTWRTENAIGVNVAGIEENFVANGGVEVTLTATTSFVLTAFSLDGTETTATVTVPID
                     PQLYVPTITPGAVAVAGGTQVVIDVNPASVPSLDGVRFAVVQSQSRGMFSRDLNVPGR
                     FIYRAGPYTGIDIIRILWVNGCGIGYTDFTANVTGETPEP"
     gene            2415..>3981
                     /locus_tag="P5164_16815"
     CDS             2415..>3981
                     /locus_tag="P5164_16815"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRY45599.1"
                     /translation="MPTEASWLGVPGTLVFALVLIGAIAAFAYTASRRWQLLTIGGPD
                     EVRWDRPLERLKGLFELGIFQKKMWWDGYAGLYHMLIFSGFVVLSVRTLSLVFEGLFP
                     KAGMPFLPAGAWQAYLLLKDVVLVTTLVGVLLALGRRYLFRKERLDPSFDAGLILVLI
                     GFLMATDLFAGAAKFALAPEHASAWEPFTAGLSGLLAGAAPATLQGVFSWCWWLHLVA
                     ILVFLNYLPFAKHFHVITALPNIFLRKLDAPGKLPTVDIEKAAEAEKFGVARVEDFSW
                     KQRLDLFTCTECGRCREVCPTHLTGKPLTPKGFLKDVRTALYTVAGDLVDTAEGRADA
                     GAEKRLEERKPLIGGWVDEETIWACTTCRYCESACPVTITYTDKIVEMRRHLVLEKSE
                     FPKEAQTAFNGMERQGNPWNLPAADRDAWTGELDFEVPVLGAMDEAERASVEVLFWVG
                     CAGSYEDRGKKVSRALARLLHDGGVKFAILGAEEACNGDSARRLGNEYLFQTLAQQNV
                     ETLNGYGVKRIVTN"
BASE COUNT          580 a         1318 c         1493 g          590 t
ORIGIN      
        1 gcgctcggcg cctcgcgcgc cgcggtggac gccggctgga tcgggcacga gcaccaggtg
       61 gggcagacgg gcaaggtcgt ctcgccgacg ctctacatcg cctgcggcat ctcgggcgcg
      121 atccagcaca tggccggcat gggctcctcg aaggtgatcg tggcgatcaa caaggacgcc
      181 gacgcgccga tcttcaaggc cgcgacctac ggcatcgtgg gcgacctgtt ccagatcgtc
      241 ccggcgatga cggaagcggt gaagaaggcg aaggcgtgag aagaatcgct ggggggtccg
      301 ggggggcgtg cgaagcccag cccccccggc gagcgaaagc gaaagcgtaa gaggacaggt
      361 gggggtccgg ggggccgcgt gcgcggcctc cggacctcgg cgaaaacgaa gagggcggcc
      421 gcgaggccgc cccttttcgt tccggcgggt ccggagcgct acggctcggg agtctctccc
      481 gtcacgttgg cggtgaagtc ggtgtagccg atgccgcagc cgttcaccca gaggatccgg
      541 atgatgtcga tcccggtgta cgggccggcc cggtagatga agcgcccggg gacgttcagg
      601 tcccgcgaga acatcccgcg gctctgggac tgcacgacgg cgaagcggac gccgtcgagc
      661 gagggaaccg aggcggggtt gacgtcgatg acgacctgcg tcccgccggc gacggcgacc
      721 gcgcccggcg tgatcgtcgg gacgtagagc tgcgggtcga tcgggacggt gaccgtggcc
      781 gtcgtctcgg tgccgtcgag gctgaaggcg gtgaggacga acgaggtcgt cgcggtgagc
      841 gtcacctcga cgccgccgtt cgcgacgaag ttctcctcga tgccggcgac gttgacgccg
      901 atggcgttct cggtccgcca ggtgaggagg aagttcgtgg agggggcgca gccgcgctcc
      961 gggatcgcct cgaagctgag gatccgcggc gctccgggga ggacgacgtg ggccgtgaac
     1021 tccgcgctcg ccccgccgca ggagccggtc gccgtgatct tcacgacgtc ggtccccggg
     1081 gaggtcccgg cggtgtagat gaaggaggtc ccctggacgg tgaaggtgga gccggtcgac
     1141 tcgcgggaga cgaactcgac ggtgaccggg cccgtcccga cggggaggat cccggtgacg
     1201 acgccgctcg tcgggagcgt ggccgaggcg ggcgccggcg tcggcggcgg cgtcgacggc
     1261 gtgatcgtga tccggagggg ccggaccgtc gactggcccg tggaggaggt ggccgtgagg
     1321 atgaagtcgc cggtggcgtg gacggtcctc ttcacggagc cggtggcggg gaggtcgttc
     1381 ccgacgccgc tgatgctcac cttcgcggcg cccgacgtct cccacgcgaa ggtcgtctcg
     1441 agcggagcgc agccggtggc cgggctcgcc gtgaagctgg cgatcgtcgg ggccgtggag
     1501 aaggcgtttc gcttgacggc gagcgggatc gccgtcccgt cgctcgtctg gttgtcgttc
     1561 cggatggcgt acggatagcc ctggccggag aggagacgca ggcggagcgc gaggcccgcc
     1621 ttgccgacgg cggcggggac gagcgtcgcg agcgaagcct gggccgcgct tccgggggcg
     1681 gtttcgatcg tgcccgaccc gacgagggcg cccgaggaga agagggcgac ctcgaggcgg
     1741 cagggctggg aagcggtgtc gacgcagacg acgccggcgt tcgtccgggc gtggccggcg
     1801 ccggtttcgg aggaggcctc ggcgccgccg ccggtcgcct cgtcgcccgg tccgagcagc
     1861 tcggagggcg cggccgcccc gaggctgacg ccgtagcgcc cggaggaggc ttcgttgtac
     1921 gtctccgacc aggccgagac ggggacgtcg gagcggacgc ggtagaggaa cgcgcccttc
     1981 tcggcgacct cgtcgggagc cggcacgagc gcgactccgt tcgccgcgaa cgtgaagggc
     2041 accgtggcga ggaccttgcc gccggccaga aactcgaccg ctcctacggc gggcgcgggc
     2101 ccggtgacgt agacgaccgt gtcgaaccgc gcgctctggt ttcccgtccc cttcgcgccc
     2161 ggcccgcccc agagctcggt cccctgcggt gcggcgaggg cgccgtgcag cacggccggc
     2221 gtcagcgcag cgagcccgag cagtacggtg agaacgagga acgacgatgt ctttctcatc
     2281 agcctggctc cgatcccgtc ggattcgttg ggccttcgaa acttagccgg catcatacac
     2341 cccgcgacgc ggccgacggg ctccgggaac gcgggtccgg cggttcccct cctgagctat
     2401 cctttccttt gatgatgccg accgaagcct cctggctcgg ggtcccgggg accctcgtct
     2461 tcgccctcgt cctcatcggc gcgatcgcgg cgttcgccta cacggcctcc cggagatggc
     2521 agctcctgac gatcgggggc cccgacgagg tccgctggga ccggcctctc gaaaggctga
     2581 aggggctctt cgagctcggg atcttccaga agaagatgtg gtgggacggc tacgccgggc
     2641 tctaccacat gctcatcttc agcggcttcg tcgtcctctc ggtgcggacc ctctctctcg
     2701 tcttcgaggg gctcttcccg aaggccggga tgccgttcct gccggccggg gcgtggcagg
     2761 cgtacctcct cctgaaggac gtcgtcctgg tgacgacgct cgtcggcgtc ctcctggcgc
     2821 tcggacggcg ctacctgttc cgcaaggagc ggctcgaccc gtcgttcgac gcggggctga
     2881 tcctcgtcct gatcgggttc ctcatggcga cggacctctt cgcgggggcg gcgaagttcg
     2941 cgctcgcccc ggagcacgcc tcggcgtggg agccgttcac ggcgggcctg tcggggctgc
     3001 tcgcgggggc cgcgcccgcg acgctccagg gcgtcttctc gtggtgctgg tggctccacc
     3061 tcgtcgcgat cctcgtcttc ctgaactacc tcccgttcgc gaagcatttc cacgtcatca
     3121 cggcgctccc gaacatcttc ctccgcaagc tcgacgcccc cggaaagctt ccgacggtcg
     3181 acatcgagaa agcggccgag gcggagaagt tcggcgtcgc cagggtcgag gacttcagct
     3241 ggaagcagcg cctcgacctc ttcacctgca cggagtgcgg gcgctgccgc gaggtctgcc
     3301 cgacgcacct gacgggaaag ccgctgacgc cgaaaggttt cctgaaggac gtccggacgg
     3361 cgctctacac ggtcgcgggc gacctcgtcg acacggcgga agggcgcgcg gacgccgggg
     3421 ccgagaagag gctcgaggag aggaagcccc tcatcggcgg ctgggtcgac gaggagacga
     3481 tctgggcctg caccacctgc cgctactgcg agtcggcctg ccccgtcacg atcacgtaca
     3541 ccgacaagat cgtcgagatg cgccggcacc tcgtcctcga gaagagcgag ttcccgaagg
     3601 aggcgcagac ggcgttcaac gggatggagc ggcaggggaa cccctggaac ctcccggccg
     3661 ccgaccgcga cgcctggacg ggcgagctcg acttcgaggt cccggtcctc ggcgcgatgg
     3721 acgaggccga gcgggcctcc gtcgaggtcc tcttctgggt cggctgcgcc ggctcgtacg
     3781 aggaccgcgg caagaaggtc tcccgtgcgc tcgcgcggct cctccacgac ggcggggtga
     3841 agttcgcgat cctcggcgcc gaggaggcgt gcaacggcga ctcggcccgc cggctcggaa
     3901 acgagtacct cttccagacg ctcgctcagc agaacgtcga gacgctgaac ggctacggcg
     3961 tgaagcggat cgtcacgaac t
//
LOCUS       DAOTEZ010000408         3977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_16783_length_3977_cov_6.104793, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000408 DAOTEZ010000000
VERSION     DAOTEZ010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16783_length_3977_cov_6.104793"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="P5164_16820"
     CDS             complement(<1..693)
                     /locus_tag="P5164_16820"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY45600.1"
                     /translation="MARRSALAVVFLAASAAACGGEAPPRTLLVAMEQDVRTLDPHRH
                     DDSVTHSVLANVFEPLVTFDPQMRVVPALAEGWSNPNDLTWRFRLRPRVRFHDGQPLT
                     SRDAKFSLERARRLRAAPHLESLVRIDAVDDETLEVGTSVPEPVLLNKLAGVGIVPAG
                     SPEELSVPVGTAAYSFVSWEPGKDLRLVANDRYWGGAPPLPEARFRVLPDAAGRAAAL
                     AKGELHLARELKR"
     gene            complement(754..1518)
                     /locus_tag="P5164_16825"
     CDS             complement(754..1518)
                     /locus_tag="P5164_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRY45601.1"
                     /translation="MSQEDQKLALFIDIDNVIIGLRESGHPKFDVKLLLSRLLDKGKI
                     IIKKAYADWSRYAEYKRAFHESAFELIDIPQHKMTGKNSADIRMVVDAMDLASNKEHI
                     TTFVIGSGDSDFSPLVSKLKENNKTVLGFGVKGSTSELLIENCDEFIYYEDLVREQVK
                     VPAVAGLPEKSREVFSLLIESMQALQRENKEILWGSMVKQTMQRKNPSFSESYYGFRA
                     FSELLESAQKHKVITLRRDAKSGSYVITGYGPGTAR"
     gene            1744..2739
                     /locus_tag="P5164_16830"
     CDS             1744..2739
                     /locus_tag="P5164_16830"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HRY45602.1"
                     /translation="MPLNESPAEGVEVLHAAAVAADLTGRGALAVSGPDAAELLHGLV
                     TNEVKGLRPGAGRHAALLTPKGRMRAELAVLRVSESELLLDCDAPLAGPLLAILQGYV
                     PFSRSVLEDRTGETGVVHLEGPAAAELLLAAALPEPGPEEYAHAAGFLDGVPLRVVRV
                     SRAGEPGFDLRTSRDAVPALLTLLSSRGAAVVSPDVLEAGRIEAGLPRWGAELTETTL
                     PNEAGLERTAISYTKGCYLGQETVARIRTYGHVNRRLVALLLPIGCAAAPGDDVRAGE
                     ETVGNVTSAVDSSRRAARVALAYVKKGHDEPGTALAVATKEGAAAGRVAAVPLER"
     gene            2729..3646
                     /locus_tag="P5164_16835"
     CDS             2729..3646
                     /locus_tag="P5164_16835"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY45603.1"
                     /translation="MSAETRAREPGAAPYVALLIVQLFFGTLPVFGKIAMREMEPFAL
                     AAFRAVFGALFLSLAARAFSGPEPRQTLREKGEVALLSLLGVVANQVLFITGLSLTTA
                     THATLLVATVPVFTILVGLLSRRERPSPRRLLAVPIAFGGVLFLLDPANLSFRHGTFV
                     GDLLVTLNSVAYAWFLVRARDVLVRRPALSFVSQAFRYGALPILLLALPGLSRLRPAE
                     LSGPAVGALVGVVLLGTVGSYALNAWALARTSASTTAFFIYVQPVFAGAFAYFALGEE
                     PSPRIFGAAALIFAGVALAIWPARKPPAS"
     gene            3731..>3977
                     /locus_tag="P5164_16840"
     CDS             3731..>3977
                     /locus_tag="P5164_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY45604.1"
                     /translation="MLSTLNLRALGWALLASALLAASVVAGSRNLQNFDGALAAYLFA
                     TLFAAFGIVYRYAVWLQRPPTRLYWRAGWRLLFSRRFP"
BASE COUNT          478 a         1498 c         1351 g          650 t
ORIGIN      
        1 cctcttcagc tcccgagcga ggtggagctc ccccttcgcg agcgccgcgg cccgcccggc
       61 cgcgtcggga aggacgcgga accgcgcctc cgggaggggg ggcgcaccgc cccagtaccg
      121 gtcgttggcc acgaggcgga ggtccttccc cggctcccac gagacgaagg agtaggccgc
      181 cgtcccgacc ggcacggaca gctcctcggg cgaaccggcc gggacgatgc cgactcccgc
      241 gagcttgttc aggaggacgg gctccgggac ggacgtgccg acctcgagcg tctcgtcgtc
      301 gacggcgtcg atcctcacga gcgactcgag atgcggcgcc gcgcggagcc gccgggcgcg
      361 ctcgaggctg aacttcgcgt cccgcgacgt cagcggctgc ccgtcgtgga agcggacgcg
      421 ggggcgcagc cggaatcgcc acgtcaggtc gttcgggttg ctccacccct cggcgagcgc
      481 cgggacgacg cgcatctgcg ggtcgaacgt gacgagcggc tcgaagacgt tcgcgaggac
      541 cgagtgcgtc acgctgtcgt cgtgccggtg cgggtcgagc gtccggacgt cctgctccat
      601 cgcgacgagg agcgtgcgcg gcggcgcctc gccgccgcac gccgcggccg atgcggcgag
      661 gaagacgacg gccagagccg accggcgcgc catgcccgat tctagagcgc ggacgcgccg
      721 gccggagggc cggtcggccg gcgcgtcacg tcttcagcgc gccgtccccg gcccgtaccc
      781 cgtgatcacg tacgagccgc tcttggcgtc ccggcggagc gtgatgacct tgtgcttctg
      841 ggccgattcg aggagctcgg agaacgcgcg gaacccgtag taggactccg agaagctggg
      901 gttcttgcgc tgcatcgtct gcttgaccat cgagccccag aggatctcct tgttctcgcg
      961 ctggagcgcc tgcatcgact cgatgaggag cgagaagacc tcgcggctct tctccgggag
     1021 cccggcgacc gccgggacct tcacctgctc gcggacgagg tcctcgtagt agatgaactc
     1081 gtcgcagttc tcgatgagga gctcggaggt cgagcccttc acgccgaagc cgaggaccgt
     1141 cttgttgttc tccttcagct tcgagacgag gggcgagaag tccgagtcgc ccgagccgat
     1201 cacgaacgtc gtgatgtgct ccttgttcga ggcgaggtcc atcgcgtcga cgaccatccg
     1261 gatgtcggcc gagttcttcc ccgtcatctt gtgctgcggg atgtcgatca gctcgaaggc
     1321 cgactcgtgg aaggcccgct tgtactccgc gtaccgggac cagtcggcgt aggccttctt
     1381 gatgatgatc tttcccttgt cgaggaggcg cgagaggagg agcttgacgt cgaacttcgg
     1441 gtgtccgctc tctcggaggc cgatgatgac gttgtcgatg tcgatgaaga gggcgagctt
     1501 ctggtcttcc tggctcatgc tcactggggg cgcggcgggg acgacggtct cgacgatcgt
     1561 cgtcgttgcg ggagagaagg cctcggtctc gaagaacgtg acgtcggctg cggggatggc
     1621 ggccgcctcc gcggcggccg tggcgcccga cttcgtcgac cggcgacggc ggcgcgtggg
     1681 acggggtttc tggctggttt cgttcaagcg tctcatatta cgacacatgc gagaatcgcc
     1741 cggatgccgc tcaacgaatc accggccgaa ggagtcgagg tcctgcacgc cgcggccgtg
     1801 gccgccgacc tgaccggccg gggcgccctc gcggtctcgg gtcccgacgc cgcggagctt
     1861 ctccacggcc tcgtcacgaa cgaggtgaag ggcctgcgcc ccggggcggg ccgtcacgcg
     1921 gcgctcctca cgccgaaggg gaggatgcga gcggagctcg ccgtcctccg agtctcggag
     1981 tccgagctcc ttctcgactg cgacgcgccc ctcgccgggc cgctcctcgc gatcctccag
     2041 ggctacgttc cgttcagccg gtcggtcctg gaggaccgga ccggcgagac cggcgtggtc
     2101 cacctcgagg gtccggccgc ggccgagctc ctgctcgccg ccgccctgcc cgagcccggg
     2161 ccggaggagt acgcgcacgc ggccggcttc ctcgacggcg tccctctccg ggtcgtccgc
     2221 gtctcgcggg ccggcgagcc gggcttcgac ctgcgcacct cgcgagacgc cgtccccgcg
     2281 ctcctcaccc tcctctcctc ccgcggggcc gcggtcgtct cccccgacgt cctggaggcg
     2341 ggacggatcg aggccggcct cccccgctgg ggcgccgagc tgacggagac gacgctcccg
     2401 aacgaggccg gcctcgagcg gacggcgatc tcctacacga aggggtgcta cctcgggcag
     2461 gagaccgtcg cgcggatccg gacctacggc cacgtgaacc ggcgcctcgt cgccctgctc
     2521 cttccgatcg gctgcgccgc cgccccggga gacgacgtgc gcgccggcga agagaccgtt
     2581 ggaaacgtga cgagcgcggt cgactcctcc cggcgcgcag cgcgggtcgc tctcgcctac
     2641 gtgaagaagg ggcacgacga gcccggaacg gcgctcgccg tggcgacgaa ggagggcgcg
     2701 gccgcgggac gcgtggccgc cgtcccgctt gagcgctgag acgcgcgccc gcgagccggg
     2761 cgccgccccc tacgtcgcgc tcctcatcgt ccagctcttc ttcgggaccc ttcccgtctt
     2821 cggcaagatc gcgatgcggg agatggagcc gttcgcgctc gcggccttcc gggccgtctt
     2881 cggggccctc ttcctctcgc tcgcggcccg cgccttctcg ggccccgagc cgcgccagac
     2941 gctccgggag aagggagagg tggcgctcct ctcgctcctc ggcgtcgtgg cgaaccaggt
     3001 cctcttcatc accggcctct cgctcacgac cgccacccac gcgacgctcc tcgtcgcgac
     3061 cgtccccgtc ttcacgatcc tcgtcgggct cctctcgcgg agagagcgcc cgtcgccgcg
     3121 acggctcctg gccgttccga tcgcgttcgg cggcgtcctc ttcctcctcg accccgcgaa
     3181 cctctcgttc cgtcacggga cgttcgtcgg cgatctcctc gtcacgctca actccgtcgc
     3241 ctacgcctgg ttcctcgtcc gggcgcgcga cgtcctcgta cgccgcccgg ccctctcgtt
     3301 cgtctcgcag gcgttccggt acggggccct gccgatcctc ctcctcgctc tcccggggct
     3361 ctcgcgcctc cgccccgccg agctctccgg cccggccgtc ggggcgctcg tcggcgtcgt
     3421 cctcctcggg acggtcggct cctatgccct gaacgcctgg gccctcgccc ggacgagcgc
     3481 ctcgacgacg gccttcttca tctacgtcca gccggtcttc gcgggcgcgt tcgcctactt
     3541 cgcgctcgga gaggagccct cgccccggat cttcggcgcg gccgccctca tcttcgccgg
     3601 cgtcgccctc gcgatctggc cggcccggaa gcccccggcc tcctgatccg gcccggaatc
     3661 cctcccgccg catgacccgc gtcatacgcg ggcccttctc agcggactcg ggcctcccta
     3721 gagtccgccc atgctctcga cgctgaacct ccgcgccctc ggctgggccc ttctcgcctc
     3781 ggccctcctc gcggcctcgg tggtcgcggg ctcgaggaac ctgcagaact tcgacggcgc
     3841 cctcgcggcg tacctcttcg cgacgctctt cgccgcgttc ggcatcgtct accgctacgc
     3901 cgtctggctc cagcgtccgc ccacgcggct ctactggcgg gccgggtggc ggctcctctt
     3961 ctcccgccgc ttccccg
//
LOCUS       DAOTEZ010000409         3966 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_16851_length_3966_cov_4.752749, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000409 DAOTEZ010000000
VERSION     DAOTEZ010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16851_length_3966_cov_4.752749"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="P5164_16845"
     CDS             complement(<1..680)
                     /locus_tag="P5164_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRY45605.1"
                     /translation="MATPIPQNTARFTPAEVARATGGVLVRDGRPLAGVSTDTRTLAP
                     GALYVALRGETFDGHRFLPQATAAGAGGVLISTAGDLPPNGAVVRVPDTRAALGDLAR
                     FHRDRWASTDGRKVVAVGGSAGKTTTTRAVAALLDVLRPGEVQSTPGNLNNDVGLPMT
                     LLLLDEHHHLAAVEIGTSHRGEIARLAAIARPDVAVLTLIAPEHTEGLGTIEDVAEEE
                     GDLFAALPP"
     gene            709..1746
                     /gene="hemE"
                     /locus_tag="P5164_16850"
     CDS             709..1746
                     /gene="hemE"
                     /locus_tag="P5164_16850"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HRY45606.1"
                     /translation="MSVPGPSRQRFVDAALGRPVDVPPVWLMRQAGRYLPEYREVRKR
                     LAFLDLCADVPSAVEVSMQPFRRFGMDGVILFSDILIPLPPMGIEVKLDEGGPKLPTP
                     VRTAAQVETLRGFDPTVGTGFVPAILRELKKEVAGRAAVIGFCGAPWTLATYAVEGGG
                     SKSFQHTKTMMWKEPKLLEALLGKLADSVGDYLAAQIEAGADIVQVFDSWAGELSKAD
                     YDRFARPATERLLSRLPKRGEVPVIHFASGSAHLIDSYAEMASDVISVDWKLPLDEAR
                     KRSRGKALQGNVDPGVLLGSPDDVKAAVRAAKSAAGEGGHVVNLGHGILPPTPPENVA
                     AFVEAAREARA"
     gene            complement(1755..3617)
                     /locus_tag="P5164_16855"
     CDS             complement(1755..3617)
                     /locus_tag="P5164_16855"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRY45607.1"
                     /translation="MPEESRPPFSWVNVPTTEPPRSVAARGLLRALCDPDRLETVFQP
                     VFRARTGELEGCEALLRVPAGSGFAGPGEAFGAALAEGLGGDLEAAAIQRALRDAEPF
                     AGDRLVFLNLLATSLADPRLGARWLVARAVAHGRVPAQLILELGQISRIVDFPALARA
                     LEPYRAEGFRIAIDDFGAGYTNLRMITDLAPDFVKLDSVFVKDVFVHARKRILVESVV
                     SLCHRINCGVIAEGIETTDDLETCLGAGVDLLQGFLLARPGAPEEAFVAEDLTVGAAG
                     GHGRGEIAAAVSHGPTLAVEAPLTAADDLFRREPELDVVPVLQHGRAVGLLARNRAET
                     FRFVSSGSGGDTVAAAIADAPWDQIEEGTPVEEVAAFVARRPRARRFEPIVVIGPGRV
                     FRGLLRLDDLLGLFARLYVENGLESHPLTDLPGRGRLEAEVDRRLTRGAPFALARLNL
                     LRLRAFNDRYGVRRGDRLLAHVAAVLRTLATEEPGSFLAHYGADDFGFLLPPERVVET
                     LRRAIQGIAATAGEFYDPEDLAAGGIAGHDARGGAILVAPAALAAGVAFWAGGGAPTL
                     RAIVTAAEAALREARRGGTQPVVRTVGPAATPAAAPERSDEPPRTQASLPKPFR"
     gene            3732..>3966
                     /locus_tag="P5164_16860"
     CDS             3732..>3966
                     /locus_tag="P5164_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HRY45608.1"
                     /translation="MPAIPIRLQGETLPMTTELLDKYNVQGPRYTSYPTAPEWTDAIG
                     PADFEAACERANAAGRPISLYVHLPFCDEQCWFC"
BASE COUNT          530 a         1458 c         1472 g          506 t
ORIGIN      
        1 ggcggaagcg ccgcgaagag gtccccctcc tcctcggcca cgtcctcgat cgtgccgagc
       61 ccctcggtgt gctcgggggc gatgagcgtc aggacggcga cgtcgggcct cgcgatcgcc
      121 gcgaggcggg cgatctcccc gcggtggctc gtcccgatct cgacggcggc gaggtggtgg
      181 tgctcgtcga ggaggaggag cgtcatcggg aggccgacgt cgttgttcag gttgccgggg
      241 gtcgactgga cctcgccggg gcggaggacg tcgaggagcg cggcgacggc gcgggtcgtc
      301 gtcgtctttc ccgcggaacc gccgacggcg acgacctttc tcccgtcggt cgaggcccag
      361 cggtcgcggt ggaagcgggc gaggtcgccg agggcggcgc gcgtgtcggg gacgcggacg
      421 acggcgccgt tcggcggcag gtcgccggcc gtcgagatca ggacgccgcc cgcgccggcc
      481 gcggtcgcct gcggaaggaa gcggtgcccg tcgaacgtct cgccgcggag cgcgacgtag
      541 agggcgccgg gggcgagcgt gcgggtgtcc gtggagactc cggcgagggg acggccgtcg
      601 cggacgagga cgccgccggt ggcgcgggcg acctcggcgg gggtgaatcg ggcggtgttc
      661 tgggggatcg gcgtagccat cgaggggccg atgctaacct cccgcgccat gtcggttccc
      721 ggcccctccc gccagcgctt cgtcgacgcc gccctcggac gccccgtaga cgtccctccc
      781 gtctggctga tgcgccaggc cggtcgctac ctccccgagt accgcgaggt ccgcaagcgc
      841 ctcgcgttcc tcgacctctg cgccgacgtc ccgtcggccg tcgaggtctc gatgcagccc
      901 ttccggcgct tcgggatgga cggcgtcatc ctcttctccg acatcctgat cccgctaccg
      961 ccgatgggga tcgaggtgaa gctcgacgaa ggggggccaa agctcccgac gccggtccgg
     1021 acggcggcgc aggtcgagac gctccgaggc ttcgacccga ccgtcgggac cgggttcgtc
     1081 ccggcgatcc tccgcgagct gaagaaggag gtcgcggggc gcgcggccgt catcggcttc
     1141 tgcggagcgc cgtggaccct cgcgacctac gcggtcgagg ggggcggctc gaagtcgttc
     1201 cagcacacga agacgatgat gtggaaggag ccgaagctcc tcgaggcgct cctcggcaag
     1261 ctcgcggact ccgtcggcga ttacctcgcc gcgcagatcg aggccggggc cgacatcgtc
     1321 caggtcttcg actcgtgggc cggcgagctc tcgaaggccg actacgaccg attcgcgagg
     1381 cccgcgacgg aacggctcct ctcgcggctc ccgaagcgag gggaggtccc cgtcatccac
     1441 ttcgcctcgg gctcggcgca cctcatcgac tcgtacgcgg agatggcctc ggacgtcatc
     1501 tccgtcgact ggaagctccc gctcgacgag gcgcggaagc gctcgcgggg caaggccctg
     1561 caggggaacg tcgacccggg cgtcctcctc ggctcgcccg acgacgtgaa ggccgcggtt
     1621 cgtgcggcga agtcggccgc cggagagggc gggcacgtcg tgaacctcgg gcacggcatc
     1681 ctcccgccga cgccgcccga gaacgtcgcc gccttcgtcg aagccgcccg cgaagcccgg
     1741 gcctgacgcc cccctcagcg gaacggcttc ggaaggctcg cctgcgtccg cggcggctcg
     1801 tccgaccgct ccggggccgc cgccggcgtc gcggccggtc cgaccgtccg gacgaccggc
     1861 tgcgtccctc cgcggcgcgc ctcccgaagc gccgcttccg ccgcggtgac gatcgcccgg
     1921 agcgtcggcg cgccgccgcc ggcccagaac gcgaccccgg cggcgagcgc ggcgggcgcc
     1981 acgagaatcg cccccccgcg cgcatcgtgc ccggcgatcc cgccggcagc caggtcttcc
     2041 gggtcgtaga actcgcccgc cgtggcggcg atcccctgga tcgcccggcg aagcgtctcg
     2101 acgaccctct cgggcggcag caggaagccg aagtcgtcgg ccccgtagtg ggcgaggaac
     2161 gagcccggct cctccgtcgc gagggtccgg aggacggccg cgacgtgcgc gaggagacgg
     2221 tcgccgcgcc ggaccccgta gcggtcgttg aacgcccgca gccggaggag gttgagccgc
     2281 gcgagcgcga agggcgcccc ccgcgtcagg cgccggtcca cctccgcctc gaggcggccg
     2341 cgtcccggaa ggtcggtgag cgggtgcgac tccagcccgt tctcgacgta gagcctcgcg
     2401 aagaggccga ggaggtcgtc gaggcgcagc agcccgcgga agacgcgccc cggaccgatg
     2461 acgacgatcg gctcgaagcg tcgcgcgcgc ggcctccgcg cgacgaacgc ggcgacctcc
     2521 tcgacgggcg ttccctcctc gatctggtcc cagggcgcgt cggcgatcgc ggcggcgacg
     2581 gtgtcgcccc cggagcccga cgagacgaac cggaacgtct ccgcgcggtt ccgggccagg
     2641 agcccgaccg cccggccgtg ctggaggacc gggacgacgt cgagctcggg ctctctccgg
     2701 aagaggtcgt ccgcggccgt gagaggcgcc tcgaccgcca gcgtcggtcc gtgcgagacg
     2761 gcggcggcga tctcgccccg tccgtgtccg cccgcggccc cgaccgtcag gtcctccgcg
     2821 acgaacgcct cctccggcgc tcccggacgc gcgaggagga acccctgcag gaggtcgacc
     2881 cccgccccga ggcacgtctc gaggtcgtcc gtggtctcga ttccctcggc gatcacgccg
     2941 cagttgatcc ggtggcagag ggagacgacc gactcgacga ggatccgctt ccgggcgtgg
     3001 acgaagacgt ccttcacgaa gacgctgtcg agcttcacga agtcgggggc gaggtcggtg
     3061 atcatccgga ggttcgtgta gccggcgccg aagtcgtcga tggcgatccg gaacccctcc
     3121 gcgcgatacg gctcgagggc acgtgcgagc gcggggaagt cgacgatccg gctgatctgg
     3181 ccgagctcca ggatgagctg cgcggggacg cgcccgtgag ccaccgcccg cgcgacgagc
     3241 cagcgtgctc cgaggcgggg gtccgcgagg gaagtcgcga ggaggttcag gaagacgagg
     3301 cggtcgccgg cgaacggctc cgcatcccgg agggcacgct ggatcgcggc ggcctcgagg
     3361 tcgccgccca gcccctccgc gagcgcggcg ccgaacgcct ctcccgggcc cgcgaagccg
     3421 gagccggcgg gaacgcgcag gagggcctcg cacccctcga gctccccggt ccgggcgcgg
     3481 aagaccggct ggaagaccgt ctcgagccgg tcgggatcgc acagggcccg gaggagcccg
     3541 cgggccgcca ccgaccgtgg cggctccgtg gtcgggacgt ttacccagga gaacggaggg
     3601 cggctctctt ccggcattgt cctgcgcctt tcccggcgtc cccgtcgccg atggggaatt
     3661 ctaggacggc cgaaccgtcc ccgacgcgcg ccgcccgcga ccggcccctc ccgtccgtgt
     3721 aatctccgcc cgtgcccgcg attccgatcc gcctccaggg cgagacgctc ccgatgacga
     3781 cggagctcct ggacaagtac aacgtccagg gcccccgcta cacctcttac ccgacggcgc
     3841 ccgagtggac ggacgcgatc gggcccgccg acttcgaggc cgcctgcgag cgcgccaacg
     3901 cagccggccg cccgatctcg ctctacgtcc acctcccgtt ctgcgacgag cagtgctggt
     3961 tctgcg
//
LOCUS       DAOTEZ010000410         3964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_16870_length_3964_cov_5.575851, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000410 DAOTEZ010000000
VERSION     DAOTEZ010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16870_length_3964_cov_5.575851"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..465
                     /locus_tag="P5164_16865"
     CDS             16..465
                     /locus_tag="P5164_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HRY45609.1"
                     /translation="MTALTDLYQEVILDHNRSPRNFRAIEGATHSGLGHNPLCGDQLT
                     LYVKVEDGTIREIGFQGKGCAISKASASLMTEAVKGKTLAEAEELFGRFHDLLTGPSD
                     VPAEAKGFGKLAVFSGVREFPVRVKCATLAWHTLHSALAGGSPASTE"
     gene            518..811
                     /locus_tag="P5164_16870"
                     /pseudo
     CDS             518..811
                     /locus_tag="P5164_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834173.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
     gene            complement(837..1631)
                     /locus_tag="P5164_16875"
     CDS             complement(837..1631)
                     /locus_tag="P5164_16875"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY45610.1"
                     /translation="MSGATLAAAREPLPLARVLRAYLQEARFEVLAALRTTGLALPFL
                     AIPVAIYLLFGVVISGSAGGGELGPGIHDYLFVGFAVLAVAMPGVFTGVTVATEREGN
                     LLKLKRALPLPPGATIVAKVAMAMVVAGIALTLVVIAALLAARVTLSPGQLLIVWAVL
                     FAGTIPFSAIGLLLGTFGSASAAPAWGNLVFLPMMWLSGLFIPLPDSLKPWVIVWPTF
                     HLDQLALGLAGVRQFTYIPPELAAAVLTGVTVLCGGLAIRRLARRG"
     gene            complement(1628..2491)
                     /locus_tag="P5164_16880"
     CDS             complement(1628..2491)
                     /locus_tag="P5164_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY45611.1"
                     /translation="MNVTKRYGTTAALDGLHLEVRPGELLAVLGPNGAGKSTAIGLWL
                     GTLQPDEGTALVMGGPPLEAASRLGVGVMMQEVALAPTLTASEHVALTSSCYRDPLSV
                     EETLGLTGTSALANRRYGRLSAGQKRQVQFATAVCGRPRLLFLDEPTVGLDVPAREAM
                     WKAIAKLRDDGCSVVLTTHYLEEAEALADRVVVLARGRIVAEGSVDEMRALVTRKRIR
                     CASSVDVGAMRSWPGVVEATRDADLVRVTASDAETVVRRLLAEDPGLRQLEVQQASLA
                     EAFTEITREAA"
     gene            complement(2687..3775)
                     /locus_tag="P5164_16885"
                     /pseudo
     CDS             complement(2687..3775)
                     /locus_tag="P5164_16885"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
BASE COUNT          642 a         1432 c         1428 g          462 t
ORIGIN      
        1 cgaggtattc ggcgcatgac ggccctgacc gacctctacc aggaggtcat cctcgaccac
       61 aaccggtcgc cccggaactt ccgcgcgatc gaaggggcga cccactccgg cctggggcac
      121 aacccgctct gcggcgacca gctgacgctc tacgtgaagg tggaggacgg gacgatccgc
      181 gagatcggct tccaggggaa gggctgcgcg atctcgaagg cctcggcctc cctgatgacc
      241 gaggccgtga aggggaagac gctcgcggag gccgaagagc tcttcggccg cttccacgac
      301 ctcctcaccg gcccgtccga cgtcccggcc gaggcgaagg gcttcggaaa gctcgccgtc
      361 ttctcgggcg tccgcgagtt ccccgttcgc gtgaagtgcg cgacgctcgc ctggcacacg
      421 ctccactcgg ccctcgccgg cggctcgccc gcctccacgg agtgacgcga tgtccgccgc
      481 cacccccgag ccgacggccg ccgcaccggc ccccgacctc gagcagagcg tcatcggcgc
      541 gctgaagaag gtctacgacc ccgagatccc ggtcaacatc tacgacctcg gcctcgtcta
      601 ctccgtcgtc gtcggggacg gcggcgccgt gaacgtcacg atgactctca ccgcgcccgc
      661 ctgcccggtg gcggggacgc tccccggcga ggtggagcgc gtcgtccgct ccgtcgaggg
      721 cgtctccgag gcgaaggtcg acctcgtctg ggacccgccg tggaaccccg ggatgatgtc
      781 gaagatggcc cgggtgatgc tggggatgtg accgcggccc gggcctccgg cccgggctat
      841 ccccgtcgcg ccaggcggcg aatcgcgagg ccgccgcaga gcaccgtcac cccggtgagg
      901 acggccgcgg ccagctcggg cgggatgtag gtgaactgtc ggacgccggc gaggccgagc
      961 gcgagctggt cgaggtggaa ggtcggccag acgatcaccc agggcttcag cgagtcgggc
     1021 agcgggatga agaggcccga gagccacatc atcggcagga agacgaggtt cccccaggcc
     1081 ggagccgccg aggccgatcc gaacgtcccc aggagcaggc cgatggcgct gaacggaatc
     1141 gtgccggcga agagcacggc ccagacgatc aggagctggc ccggtgagag ggtgacccga
     1201 gcggcgagaa gcgcggcgat gacgacgagc gtgagggcga tgccggcgac gaccatcgcc
     1261 atggcgacct tcgcgacgat cgtcgccccg ggcgggagcg gcagggcccg cttgagcttc
     1321 agcaggttcc cctcgcgctc ggtcgccacc gtcacgcccg tgaagacccc cggcatcgcc
     1381 acggcgagca ccgcgaagcc gacgaacagg tagtcgtgga tccccggacc gagctcgccg
     1441 ccgcccgcgc tgccggagat cacgacgccg aacagcaggt agatcgccac cgggatcgcg
     1501 aggaacggca gcgcgagacc cgtcgtgcgc aacgcggcga gcacctcgaa cctcgcctcc
     1561 tggagatagg cgcggaggac ccgggcgagc gggaggggct cgcgggccgc ggcgagcgtc
     1621 gcgccgctca cgcggcctcc cgggtgatct cggtgaacgc ctccgcgagg ctcgcctgct
     1681 ggacctcgag ctggcgcagg ccggggtctt ccgcgagcag ccggcgaacg accgtctcgg
     1741 cgtcggacgc cgtgaccctc acgaggtcgg cgtcgcgggt cgcctcgacg acgcccggcc
     1801 agctcctcat cgcgccgacg tcgacgctcg aggcgcagcg gatccgcttg cgcgtcacca
     1861 gggcgcgcat ctcgtcgacg ctcccctccg cgacgatccg cccccgggcc agcacgacga
     1921 cacggtcggc gagcgcctcg gcctcctcga gatagtgggt cgtgagcacg accgagcagc
     1981 cgtcgtcgcg cagcttcgcg atcgccttcc acatggcctc gcgcgccggg acgtcgagcc
     2041 cgaccgtcgg ctcgtcgagg aacaggagcc gcggccggcc gcagacggcc gtcgcgaact
     2101 ggacctgccg cttctggccc gccgagagcc tgccgtacct ccgattcgcg agcgcgctcg
     2161 tgccggtgag accgagcgtc tcctcgacgg agaggggatc gcggtagcag ctcgacgtga
     2221 gggcgacgtg ctcgctcgcc gtgagcgtcg gagcgagcgc cacctcctgc atcatcacgc
     2281 ccacgccgag ccggctcgcg gcctcgagcg gcggaccgcc catcacgagc gccgtccctt
     2341 cgtccggctg cagcgtgccg agccagaggc cgatcgccgt cgacttgccg gcgccgttcg
     2401 gaccgagcac cgcgaggagc tccccggggc ggacctcgag gtggaggccg tcgagcgccg
     2461 ccgtcgttcc gtacctcttc gtcacgttca cgagcgcggc gaccgcgccc gcatccgagg
     2521 cacggccttc gcgccggctc tccacgtctc gaggagccgg cggcagcgcg gcctcgcgcc
     2581 cttcggcgct cgacccccgc cgcggatggc gccttccgtt caggagctcg ccgagcgcgg
     2641 tcgatcgcac gaggacgtgg tagctcgaaa ggagcagcgc catcgtcacg acgaggatcg
     2701 ccgggagctt gatcgaccag tggagcggca catccatcag catcacctga aggccgaaga
     2761 cgatcggcgg atgcaccagg tagagccagt acgacgcgtc ggagaggtac cggcgcacga
     2821 cgctttcccc ggacaggaag cggaccgcag cccccaggag cccgaacgtc cacgtccaga
     2881 tcgagagcgc gtacgcggcc gcataggccc atcgcatcgg cgccccgccc tcgatcccgc
     2941 cggtcgtcgc cgccgcggag aggtccggcc ggaggccggc gatggcgaga cagaccgacg
     3001 tgagagccgc cgccagggcg agattcgccg gccactgccg cgcgagcgcc tcgagccggt
     3061 cgacgcgccg gttcagcagc cacccgagcc cgaacgccgt accgtaggcg accatcgccg
     3121 gaagctgcgg cgcgaggccg atgtcgggcg tcgggatccc gaaccaggcc acccagtccg
     3181 ccttctgcga gaggaccgcg accgtcggcg ccgcgaggag cagcggcgcg agacggctcg
     3241 cgagcgcgcg gtcggcccac cggtcgattc tggcccggag cttctcccct tcgtcgagcg
     3301 ccacgacccc ggcgcgcagc gcgagcgcca ggacatagaa gatcgcgagg tcgtagagga
     3361 accagagatg gacgagcggg acgccccgtg gcccggcggc cccgcccggg tcgccgtcgg
     3421 gaaacgtccg cgtcagcccc caggccacgg ccgcgatcgt cggcggcgcc aggatcagcc
     3481 agccggcggt gagcggcacc aggactcgcc gggcgcggtc cttcacgaag ccgcggacgc
     3541 cgcggcgatg gaggagcagg cgggcgaaga agccggcgat gacgaagaag agcgtcatcc
     3601 ggaacgtgtg gatgacgaag aacgcgacgg cgggcgccgt tccctgcgag acgtcccggg
     3661 ccgggacgac gaagaagaac gacatcgtcg cgtggaggac gatgccgagg aggagcgccc
     3721 cggcgcgaac ggcgtccaat gcgtgcaggc ggtcgggatt cgtcgggcga ttcatggccg
     3781 gctcctcaga acgcgtctct gcgacgcgac ggaacgtaga aggcgagggc cagagacagc
     3841 aggatcaggg cgacctcgac cgccagcacg gtcaggattt cgggagtcca ggtcgcgacg
     3901 gcctccttcg agcgcccggc gacacccgtg agccgggcga gccgggacag gaagatcgtc
     3961 accg
//
LOCUS       DAOTEZ010000411         3954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_16918_length_3954_cov_6.586561, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000411 DAOTEZ010000000
VERSION     DAOTEZ010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16918_length_3954_cov_6.586561"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="P5164_16890"
     CDS             <1..857
                     /locus_tag="P5164_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45612.1"
                     /translation="MSDAAILTERPTVLASLASARGDEPPPRLLPLPGSELPPGSTSS
                     DPVESGPSMTAWAIPWTFTRFGVASVHEADYDAALPRAQLEWAMLLEKAPPAGTLAAA
                     LARSAGAVAVVAPEASPGGRPVARLLPVASPVPPVRFVSRVVREPGPLAASTRFLRDR
                     APVDAAVLSAPGPPLVPSAGRVLRVSDRPDRLEVEVEVAGPAPGYLYVARPFVAARRA
                     TLDGRPAVVEDANVGYTGLAVPPGRHVVRLRPSGGWLMIAAVMTILGLLALVALLRRN
                     PSPRAASA"
     gene            854..2281
                     /locus_tag="P5164_16895"
     CDS             854..2281
                     /locus_tag="P5164_16895"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRY45613.1"
                     /translation="MSLSRNTRRRLALYGGAALVAAAALAGPKLVRSRAPRVEMRSLD
                     LAEMRRHEATRLLREYVGIDSQNPPGRTVETARFWAEKLGCEGIPFEIVGDDPERPIV
                     VARLSGRTRGDALLLLHHMDVFPVGDLSAWDLPPFAAADGVGDLVQYVCGRGTLDMKG
                     QGIADFLAIASLRRDGIVPERDVVFVAEPGEETYTPEIGIGWVLENRPDLLDGVTDVL
                     NEGGVNEVSGDRIARFGIEVLQKSNVIATAHARDEAALRALRDLLERKMSEEPFRVLD
                     EVQDFLSFVGPSRSDIWGHQMNDARAAVASGRLAEGIPEVYRSLFRDMYYWGEPKRAA
                     TGGGFSMDLVATLLPGRSARARWEEMERWAAPLGVRLTLRHVTPDSVPAERRGRAWET
                     LHTVLALDPDEDADVGPYVLTGSYTNLSYLRARGIRSYGVSPFAVNLLDAVTIHSKNE
                     RIRLPAYLEGVERTKRIVREYALQP"
     gene            2365..2634
                     /locus_tag="P5164_16900"
     CDS             2365..2634
                     /locus_tag="P5164_16900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45614.1"
                     /translation="MLEKTKARVTEERSAKATPLKDADIRALLAAVDEVVIAKGKKAA
                     SRSAREVVLDDLRGPTGNYRAPMLRVGRRLLVGFHPEALADLLGS"
     gene            complement(2747..3241)
                     /locus_tag="P5164_16905"
     CDS             complement(2747..3241)
                     /locus_tag="P5164_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005363854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HRY45615.1"
                     /translation="MKKTIAASAAALLLAVSVAAPARADHHPGKDIVETAVAAGSFST
                     LATALKAAGLVETLKGKGPFTVFAPTDDAFKKLPAGTLEKLLADKARLTKVLTYHVVS
                     GKVMAADVVKLSEAKTVEGSSVKIAVKDGKVKLNDANVVKTDVGASNGVIHVIDTVIL
                     PPAK"
     gene            3356..>3954
                     /locus_tag="P5164_16910"
     CDS             3356..>3954
                     /locus_tag="P5164_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRY45616.1"
                     /translation="MEKVHIDGVEIALAHPDEFPLEWKGRRDLLDQLLAAWLVVEKGD
                     LPLHPRLVGKPGVGKTTLAYAAAKSIGRPAWVFQATMDTRPEDLLVTPVVAGEGKIRY
                     VASGVVTAMLKGGVAILDEGNRMSEKSWASLAPLLDARRTVESIVAGVKIRAHPDFRF
                     VTTMNDDASTFELPDYVNSRLTPTILVDFPDEEEELAILA"
BASE COUNT          523 a         1412 c         1422 g          597 t
ORIGIN      
        1 cgatgtcgga cgcggcgatc ctgacggagc ggccgaccgt gctcgcctcg ctggcgtcgg
       61 cccggggcga cgagccgccg ccgcgactcc tgcccctgcc cgggtcggag cttccacccg
      121 gctccacgtc gagcgatccg gtcgaaagcg gcccctcgat gacggcctgg gcgatcccgt
      181 ggacgttcac ccgcttcggc gtcgcctccg tccacgaggc ggactacgac gcggcccttc
      241 cgcgcgccca gctcgagtgg gcgatgctgc tcgagaaggc cccgcccgcc ggaactctcg
      301 ccgcggcgct tgcccgttcc gccggcgcgg tcgccgtcgt cgcccccgag gcatcgccgg
      361 gcgggcggcc cgtcgcgcgg ctccttcccg tcgcgagccc ggtcccgccc gtccggttcg
      421 tgtcgcgtgt cgtgcgcgag cccgggccgc tcgcggcctc gacgcgcttc ctccgcgatc
      481 gggccccggt cgacgcggcc gtgctctccg ctcccgggcc tccgctcgtc ccttcggccg
      541 ggcgggtcct gcgcgtctcg gaccgtcccg accggctcga ggtcgaggtc gaggtcgccg
      601 gccccgcgcc cggctacctg tacgtcgccc gtcccttcgt cgccgcccgg cgcgccacgc
      661 tcgacggtcg gccggccgtc gtcgaagacg ccaacgtcgg ttacacgggc cttgcggttc
      721 cgcccggccg gcacgtcgtc cgtttgcggc cttcgggggg atggctgatg atcgcggccg
      781 tgatgacgat cctcgggctc ctcgccctgg tcgctctcct tcgccggaac ccgtcgcccc
      841 gcgcggcgag cgcatgagcc tctcccggaa cacgcgtcgc cgcctcgcgc tctacggcgg
      901 cgccgcgctc gtcgcggccg ccgcgctcgc cgggccgaag ctcgtccggt cccgcgcgcc
      961 gagggtggag atgcggtcgc tcgacctcgc cgagatgcgg cggcacgagg cgacgcgcct
     1021 ccttcgcgag tacgtcggga tcgactcgca gaaccccccg ggccggacgg tcgagacggc
     1081 gcgcttctgg gccgagaagc tcggctgcga ggggatcccg ttcgagatcg tcggagacga
     1141 cccggagcgt ccgatcgtcg tggcgcggct ctcgggtcgc acccggggag acgcgctgct
     1201 tctcctccat cacatggacg tcttccccgt cggggacctc tctgcctggg acctcccccc
     1261 cttcgccgcc gcggacggcg tcggcgacct cgtccagtac gtctgcgggc gcgggacgct
     1321 cgacatgaag ggacagggga tcgccgattt cctcgcgatc gcgtcgctcc gcagggacgg
     1381 aatcgtcccc gagcgggacg tcgtcttcgt cgccgagccg ggggaggaga cctacacgcc
     1441 cgagatcggg atcggatggg tcctggagaa ccgtcccgac ctcctcgacg gagtgaccga
     1501 tgtcctcaac gaaggcggcg tgaacgaggt cagcggggac aggatcgccc ggttcggcat
     1561 cgaggtcctc cagaagtcga acgtcatcgc cacggcgcat gcccgggacg aagccgccct
     1621 tcgggcgctt cgggacctcc tcgagcggaa gatgtccgag gagccgttcc gggtcctcga
     1681 cgaggttcag gacttcctct ccttcgtcgg cccctcgcga tccgatatct ggggacacca
     1741 gatgaacgac gcccgggcgg ccgtcgcgtc ggggaggctg gccgagggga tcccggaggt
     1801 ctaccggtcg ctctttcgcg acatgtacta ctggggcgag ccgaagcgcg cggcgacggg
     1861 gggcggattc tccatggacc tggtcgcgac gctgctgccg ggccgttcgg cacgtgcccg
     1921 ctgggaggag atggagcgat gggcggcgcc gctcggcgtg cgcctgacgc tgcggcacgt
     1981 cacccccgac agcgtcccgg cggagcggcg aggtcgcgcg tgggagacgc tccacacggt
     2041 cctcgcgctc gatccggacg aggacgcgga cgtcggaccg tacgtcctga cggggagcta
     2101 cacgaacctc tcctacctgc gggcgcgcgg catccgctcc tacggcgtct cgccgttcgc
     2161 ggtgaacctc ctggacgccg tgacgatcca cagcaagaac gagaggatcc gccttcccgc
     2221 gtacctggaa ggggtcgaga gaacgaaacg gatcgtccgc gagtacgccc tgcagccttg
     2281 agggcgcgga aggaggaggg tatgccgacc gtcggcttct attaccagcg cccggggtga
     2341 acgagctgcg gacgcacccg tgaggtgctc gagaagacga aggcccgggt tacggaggaa
     2401 cgttccgcga aggcgactcc tctgaaggac gccgacatcc gcgcgctcct cgccgcggtc
     2461 gacgaggtcg tgatcgcgaa ggggaagaag gccgcctcgc gaagcgcgcg ggaggtcgtc
     2521 ctcgacgacc tcaggggtcc gaccgggaac tacagggcgc cgatgcttcg cgtcggccga
     2581 aggctcctcg tcggcttcca tccggaggct ctcgcggacc tgctggggag ctgaccggga
     2641 gcgggccggg cgctcccgcg ccgtccgggg gaacgggaga aggcggccgc gggatgccgc
     2701 ggccgccctc cgtcgtacgg ggccgtccgg ccgggaaggg ccgcggctac ttcgcgggcg
     2761 ggaggatgac cgtgtcgatc acgtggatga cgccgttgga ggccccgacg tcggtcttca
     2821 cgacgttggc gtcgttcagc ttcaccttgc cgtccttcac ggcgatcttc accgaggagc
     2881 cttcgacggt cttcgcctcg gagagcttga cgacgtcggc ggccatcacc ttgccggaga
     2941 cgacgtggta ggtgaggacc ttcgtgagcc gggccttgtc ggcgaggagc ttctcgagcg
     3001 tgccggcggg gagcttcttg aacgcgtcgt cggtcggcgc gaagacggtg aagggacctt
     3061 tccccttcag cgtctcgacg aggccggcgg ccttcagcgc cgtggcgagc gtgctgaagc
     3121 tgccggcggc gacggcggtt tcgacgatgt ccttgcccgg gtggtggtcc gcgcgagcgg
     3181 gggccgcgac ggagacggcg agcagaaggg cagcggcgga cgcggcgatg gtcttcttca
     3241 ttgtctttct ccggtacggg cgcctcgggc gcccttcgcg gcaatctacg gaggggcgcg
     3301 ccccggcgga ttgcggcggc gtcgcggagg ccggctgcgc ccctagaatc cgtccatgga
     3361 aaaggtccac atcgacggcg tcgagatcgc cctcgcgcac cccgacgagt tcccgctcga
     3421 gtggaagggg cggcgcgacc tcctcgacca gctcctcgcc gcgtggctcg tcgtcgagaa
     3481 gggggacctc ccgctccacc cccgtctggt cggaaagccc ggggtcggca agaccacgct
     3541 cgcctacgcc gccgcgaaga gtatcgggcg ccccgcctgg gtcttccagg cgacgatgga
     3601 cacccgcccc gaggacctgc tcgtcacgcc ggtcgtcgcg ggcgaaggaa agatccgcta
     3661 cgtcgcctcc ggcgtcgtca cggcgatgct gaaggggggc gtcgcgatcc tcgacgaggg
     3721 gaaccggatg agcgagaagt cgtgggcctc gctcgcgccg ctcctcgacg cgcgccggac
     3781 cgtcgagtcg atcgtcgcgg gcgtgaagat ccgggctcac cccgacttcc gcttcgtcac
     3841 gacgatgaac gacgacgcct cgacgttcga gctgcccgac tacgtcaact cgcgcctcac
     3901 gccgacgatc ctcgtcgact ttcccgacga ggaggaggag ctcgcgatcc tcgc
//
LOCUS       DAOTEZ010000412         3947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_16962_length_3947_cov_6.600206, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000412 DAOTEZ010000000
VERSION     DAOTEZ010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16962_length_3947_cov_6.600206"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="P5164_16915"
     CDS             <1..939
                     /locus_tag="P5164_16915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45617.1"
                     /translation="LKAFEGTSLYVQAGKAPRFAKQTTRRLESYGLWGTAVNRFEEIG
                     VEAGGSLGRHVYWRAAAANGNPLFLRDTNSLAGDNGTPERVDLARLGEAPYNSGFPIF
                     YDAQANDTRLSGKAQAGGGLGVRFVSDDGRRGIDVLGWAFHRKLADRVDVEGSLYGGD
                     LDLLDDGMAGQVNLLDGRDKTEAGVNVEARWERLSLFGQYVRQEVAGLVRKGFEVEAS
                     YRIPLNGLFAVGDQPWLNWVQPAVRVSRIENDFSVPAGSFSPSFAWDWTKIDFGFRVG
                     IVRNVDLTAEYARHDMEVWGWTLHPDELLVTLRAGF"
     gene            965..1357
                     /locus_tag="P5164_16920"
     CDS             965..1357
                     /locus_tag="P5164_16920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45618.1"
                     /translation="MKALAYLVGLLLSAPVLAFALGVLALGHAIATRNVFTLLYQLVQ
                     VFAWALPLTALAAVALVALVACAFFPRGRLAGAGMLVALNVAALVVVLRATGLPREGG
                     EALFFLPTVVAVGLHGTLLLRRHRPRAA"
     gene            complement(1403..1978)
                     /gene="efp"
                     /locus_tag="P5164_16925"
     CDS             complement(1403..1978)
                     /gene="efp"
                     /locus_tag="P5164_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRY45619.1"
                     /translation="MASLIEAIEIKKKTFFEHEGDPYACLDVTVSTPTARGGQTLVRL
                     KMRNLKTRAVFDKTFKAGDKFKEPDLGKVQATFLYSDADGFHFMDQESYDTLTLSAEL
                     IGDNRFLLVENTVVGLLKFNGSPIDLDLPPHVELTVTYTEPGHRGDTSSGGVTKAATL
                     ETGMEIRVPLFVKEGDRVKVHTETREFSGRA"
     gene            2295..>3947
                     /locus_tag="P5164_16930"
     CDS             2295..>3947
                     /locus_tag="P5164_16930"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HRY45620.1"
                     /translation="MPGRRDPLLEKVAAAPTVAGLLSRLEARVLEDAAGGVSLVGAPQ
                     GLVPYLFGAASAALGLKWVVVCAHERDAAALQRDAAAVFGAERIAWFPAPSLTPYQGI
                     PPSLKVRREEYGALSRLAEGSVELLVVPARALLRILPSPEDVRRRSLAVAAGDRLDVG
                     RLVASLVAEGYARADLVTECGDLAVRGGLVDVFPPNLPEPVRIELGFEEVESVRTFDP
                     DTQRSTGLLDRVLLPPMAATPDTHRSRAQALEVLAACRTEDDEPPVPRDPDAPRKQDG
                     LEELLPLLAGGASTASILDHAPSFALAVDDPVAVAEELARAADLLRLDYEKVRKQGKA
                     LPDPIRLAGDPDRTAELVRSRALVAFAPTVPEGESVTIGAEAVLSFEDRLPDAPREIE
                     RARRDGFAVVLSAPSKGEREHLARLLAEYEVDFAGADGDVTAPLAPGACRIVEGGPAS
                     GFLFRSAGLLLLTAVDVLGEPRAAAPRRRAASEAFLSDLRDLKPGDVVVHRDYGLGLF
                     RGLVTIRDGEVDREMVDLRYAGDAKLLVPVERLDLLQKYQSGG"
BASE COUNT          486 a         1442 c         1431 g          588 t
ORIGIN      
        1 ctgaaggcgt tcgaggggac gtcgctctac gtccaggcag ggaaggctcc ccgattcgcg
       61 aagcagacga cccggcgcct cgagagctac ggcctctggg gcaccgcggt caaccggttc
      121 gaggagatcg gcgtcgaggc gggcgggagc ctcgggaggc acgtctactg gagggcggcc
      181 gcggcgaacg ggaacccgct cttcctgcgc gacacgaact cgctcgccgg agacaacggg
      241 acgccggaac gggtggacct cgcgcgcctc ggcgaggcgc cctacaactc cggcttcccg
      301 atcttctacg acgcccaggc gaacgacacg cgcctctccg ggaaggcgca ggccgggggc
      361 ggcctcggcg tccgcttcgt gtcggacgac ggacggaggg ggatcgacgt cctcgggtgg
      421 gccttccacc ggaagctggc ggaccgggtc gacgtcgagg ggagtctcta cggcggcgac
      481 ctcgacctcc tcgacgacgg gatggcgggg caggtgaacc tgctcgacgg acgcgacaag
      541 accgaggcgg gcgtgaacgt cgaggcgcgc tgggagcgcc tctcgctctt cggccagtac
      601 gttcggcagg aggtcgccgg cctcgtgagg aaggggttcg aggtcgaagc gtcgtaccgc
      661 atcccgctga acgggctctt cgccgtcggc gatcagccgt ggctgaactg ggtccagccg
      721 gccgtccgcg tctcgcggat cgagaacgac ttctcggtcc ccgccggctc gttctcgccg
      781 tccttcgcgt gggactggac gaagatcgac ttcggcttcc gcgtcggaat cgtccggaac
      841 gtcgacctga cggccgagta cgcgcggcac gacatggagg tctggggatg gacgctccac
      901 cccgacgagc tcctcgtcac gctccgcgcc ggcttctgac tccgccgccc cgagccgtgg
      961 gcgtgtgaag gccctcgcct acctcgtcgg gctcctcctc tcggcgccgg tcctggcttt
     1021 cgcgctggga gtcctcgcgc tcggccacgc gatcgcgacc cgcaatgtct tcacgctcct
     1081 ctaccagctc gtccaggtgt tcgcgtgggc ccttccgctc acggcgctcg cggccgtcgc
     1141 gctcgtcgcg ctcgtcgcgt gcgcgttctt cccccggggg cggctcgccg gcgcggggat
     1201 gctcgtcgcg ctgaacgtcg cggccctcgt cgtcgtactg agggccacgg gcctgccccg
     1261 cgagggaggt gaggcgctct tcttcctgcc gacggtcgtc gccgtcggtc tgcacggcac
     1321 gctgctgctg cggcgccacc ggccccgcgc ggcgtgagcc ccgcgatccg ggccgccatc
     1381 ccccgcggcc cgcgcttccg gctcaggccc ggccggagaa ctcgcgggtc tcggtgtgga
     1441 ctttgacccg gtccccttcc ttgacgaaga gagggacccg gatctccatt cccgtctcga
     1501 gcgtcgccgc cttcgtcacg ccgccgctcg acgtgtcgcc gcggtggccg ggctcggtgt
     1561 acgtgacggt gagctccacg tgcggcggga ggtcgaggtc gatcgggctc ccgttgaact
     1621 tcagcagccc gacgacggtg ttctccacga ggaggaaccg gttgtcgccg atcagctccg
     1681 ccgagagcgt gagcgtgtcg tacgactcct ggtccatgaa gtggaagccg tcggcatcgg
     1741 agtagaggaa cgtggcctgg accttcccga ggtcgggctc cttgaacttg tcccccgcct
     1801 tgaacgtctt gtcgaagacg gcgcgggtct tcaggttgcg catcttcagc cggacgagcg
     1861 tctgcccgcc gcgcgccgtc ggggtcgaga ccgtcacgtc gaggcaggcg tacgggtcgc
     1921 cctcgtgctc gaagaacgtc ttcttcttga tttcgatcgc ttcgatgaga ctcgccatgt
     1981 gtcctcttcg ccgcgccggg actggacccg cggccggccg gggcgggatt ctagggcctc
     2041 ggccattccg gcgccggacg gcagggacga ccggggagac cggcccacag ccggggccgg
     2101 ccggaaaccg tgaggtcgac gaacctcggt gccaggcgtg aaatcgtgta ttgtcataga
     2161 caatacacga tttcacgcct ggcaccgcga gggtggcgcc gcgagggggg caccgagggg
     2221 gggcgccgcg gggggccctg gaggggcgga gggggtgccg gagcggggga cgggggcgcc
     2281 gcataatcgg cgccgtgccg ggccggaggg accctcttct cgaaaaggtc gccgccgcgc
     2341 ccacggtggc ggggctcctc tcgcgtctcg aggcccgggt cctcgaggac gccgcgggag
     2401 gggtgtcgct cgtcggcgcc ccccagggcc tcgtcccgta cctcttcggc gccgcctccg
     2461 cggccctcgg cctgaagtgg gtcgtcgtct gcgcgcacga gcgggacgcc gccgccctcc
     2521 agcgcgacgc ggccgccgtc ttcggcgcgg agcggatcgc gtggttcccg gcgccgtcgc
     2581 tgacgcccta ccaggggatc ccgccgtcgc tgaaggtccg gcgggaggag tacggggcgc
     2641 tctcgcgcct cgcggagggg agcgtcgagc tcctcgtcgt ccccgcccgc gccctcctcc
     2701 ggatccttcc gagccccgag gacgtgcggc ggagaagcct cgcggtcgcg gccggagaca
     2761 ggctcgacgt cggccggctc gtcgcctcgc tcgtcgccga ggggtacgcg cgggcagacc
     2821 tcgtcaccga gtgcggcgac ctcgccgtcc gcggcggcct cgtcgacgtc ttccctccga
     2881 acctcccgga gcccgtgcgg atcgagctcg gcttcgagga ggtcgagtcg gtccggacgt
     2941 tcgaccccga cacgcagcgc tccacggggc tcctcgaccg ggtcctcctc ccgccgatgg
     3001 cggcgacgcc cgacacgcac cggagccgcg cgcaggcgct cgaggtcctg gccgcctgcc
     3061 ggacggagga cgacgagcct cccgtcccgc gcgaccccga cgcgccccgg aagcaggacg
     3121 gcctcgagga gctcctcccg ctcctcgcgg ggggcgcgtc gaccgcgtcg atcctcgacc
     3181 acgcgccgtc gttcgccctc gcggtcgacg accccgtggc ggtcgccgag gagctcgccc
     3241 gcgcggccga cctcctccgg ctcgactacg agaaagtacg caagcagggg aaggcgcttc
     3301 ccgacccgat ccgcctcgcc ggcgacccgg accggacggc ggagctcgtc cggtcccgcg
     3361 ccctcgtcgc cttcgcgccg accgtgccgg aaggggagtc ggtcacgatc ggggccgaag
     3421 cggtcctctc cttcgaggac cgtctccccg acgcgccgcg cgagatcgag cgcgcccggc
     3481 gtgacggctt cgccgtcgtc ctctcggcgc cgtcgaaggg ggagcgcgag cacctcgcgc
     3541 ggctcctcgc cgagtacgag gtcgacttcg cgggggcgga cggggacgtc acggcgcccc
     3601 tggcgccggg ggcctgccgc atcgtggagg gaggaccggc gagcggcttc ctctttcgct
     3661 ccgcgggcct tcttctcctc accgccgtcg acgtcctcgg cgagccgagg gccgccgctc
     3721 cgcgccgaag ggccgcgtcc gaggcgttcc tctcggacct gcgcgacctg aagccggggg
     3781 acgtcgtcgt ccaccgcgac tacggcctcg gcctcttccg cggcctcgtg acgatccggg
     3841 acggcgaggt ggaccgcgag atggtcgacc tccgctacgc gggggacgcc aagctcctcg
     3901 tcccggtcga gcggctcgac ctcctccaga agtaccagag cggcggc
//
LOCUS       DAOTEZ010000413         3940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_17009_length_3940_cov_6.429601, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000413 DAOTEZ010000000
VERSION     DAOTEZ010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17009_length_3940_cov_6.429601"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3940)
                     /locus_tag="P5164_16935"
     CDS             complement(<1..>3940)
                     /locus_tag="P5164_16935"
                     /inference="COORDINATES: protein motif:HMM:NF012433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HRY45621.1"
                     /translation="SDNVVVGTNLFVSDLVLAVRPDPETGAVTARVLSGATGRAVAGA
                     EVTLWRFDWREGHRRAEAKASDSDGRVSFALREQGGAFHLLAKKGRDLTFDGQQVGFH
                     RESKPGLRSDSLLYTDRSIYRPGQTLHWKVLAYRGSAEDARFRVADRTPVTVWLVDPN
                     GERVETRTVSTNDFGTAAGEMVVPTGRLLGAWRLESSLSGSTSVRVEEYKRPTFEATF
                     LEPKEPMRLNRKASLVGVARYYFGLPVTNGSVKWRVTRETLRPWWWAFWGFGSVAKPQ
                     VVAAGETTLDAEGTFRVSFTPEADETKADSKETTWRYVVQADVLDEGGETRSATRAVR
                     LGFVSVEADVKFPSGFGRAGEAMSARITRTNLDGAPRPGNGTWRLIALAPPAETPLPA
                     DLPRRVEQAEEGEGASVRTPGDALRPRWDTAFRVEAVLRDLPDGAEKARGEARHGADG
                     VAALDLGTLPAGAWRIRYETVDDFGAKYETSRDVIVAGGKAAPPVPLVLAAESGSVVV
                     GGTARLLAASGLPGQEMLLERFRAGKLLSRTVLTAGKDPTVVEFPVTEADRGGFGVTL
                     TALRDHQWMSQAAAVYVPWDDRELKLEFATFRDRIRPGTRETWRVKVTSPSGKPAEAG
                     AAELLAYMYDRSLDVFAPHTPPSILSLYPSRTGTAWTRATLGAGRAQWVRGSFAPTPS
                     GPSFEPDRLLELDRYGIGGPGARGRKFAMRGGMGVTMVSEAALERDGVPAPAPAAAPQ
                     MAKSVEMKVEDAAANEARTEAGVAPAAEAPALRSNFAETAFFTPQLLTGADGSASIEL
                     TVPDSVTSWNVWLHAVTKDLRGASLKRETKSVKELMVRPYVPRFLREGDRAELKVVVN
                     NASEGTLAGSLVLQVLDPETNEDLTARFGLPVADARRAVAVPAGGGVNETFHLIAPPG
                     VRTVAFKVTATSGNLSDGELRPLPVLPSRMHLVQSRFVTLREGQRKEMRFEDLAKGGD
                     PTLLNEQMVVTVDAQLFYAVLDALPYLVNYPYECTEQTLNRFVSTGIVSSMFEQYPSV
                     AAMAKELSKRDVRLETWDAADPNRKMTLEESPWLVDAKGGKDAGEGLAKVLDPRVAKA
                     EREAALAKLAKAQTSIGGFPWWPGGQPSPYMTVYILHGLAKATEFGVEVPKEMVQRAW
                     GYVAKHYRDDLLSLMRKDCCWELLTFVNYVASAFPDPSWSENGLTHAERREILAFSFK
                     HWKEHSPYLKGYLALTLKRANRLKDAQLVFASVMDSAKTTEELGTFWAQEDRSWLWYN
                     DTIETHAFALRTLTELTPKDPRRDGLVQWLFLNKKLNQWKSTRATAEVLYS"
BASE COUNT          535 a         1415 c         1413 g          577 t
ORIGIN      
        1 gagtagagga cctcggccgt cgcccgggtc gacttccact ggttcagctt cttgttcagg
       61 aagagccact ggacgaggcc gtcgcgccgc gggtccttcg gcgtcagctc cgtgagcgtc
      121 cgaagggcga aggcgtgggt ctcgatcgtg tcgttgtacc agagccagga gcggtcctcc
      181 tgcgcccaga aggtcccgag ctcctcggtc gtcttcgccg agtccatgac gctcgcgaag
      241 acgagctggg cgtccttcag ccggttcgcc cgcttcagcg tcagcgcgag gtagcccttg
      301 aggtagggcg agtgctcctt ccagtgctta aaggagaacg cgaggatctc cctgcgctcc
      361 gcgtgcgtga ggccgttctc ggaccaggaa ggatcgggga aggcgctcgc gacgtaattg
      421 acgaacgtca ggagctccca gcagcagtcc ttccgcatca gggagaggag gtcgtcgcgg
      481 tagtgcttcg cgacgtagcc ccaggcccgc tggaccattt ccttcgggac ctcgacgccg
      541 aactccgtcg ccttcgcgag gccgtggagg atgtagaccg tcatgtaggg ggacggctgg
      601 ccgcccggcc accaggggaa cccgccgatc gaggtctggg ccttcgcgag cttcgcgagg
      661 gccgcctcgc gctccgcctt cgcgacgcgc gggtcgagga ccttcgcgag gccttcgccc
      721 gcgtccttcc cgcccttcgc gtcgacgagc cacgggctct cctcgagcgt catcttccgg
      781 ttcgggtcgg ccgcgtccca cgtctcgagg cggacgtccc tcttcgagag ctccttcgcc
      841 atcgcggcga ccgaggggta ctgctcgaac atcgaggaga cgatgccggt cgagacgaag
      901 cggttgagcg tctgctcggt gcactcgtag gggtagttca cgaggtacgg gagcgcgtcg
      961 aggacggcgt agaagagctg ggcgtcgacg gtgacgacca tctgctcgtt gaggagcgtc
     1021 gggtctcccc ccttcgccag gtcctcgaag cgcatctcct tccgctgccc ttcgcggagc
     1081 gtgacgaagc gcgactggac gaggtgcatc cggctcggaa ggaccggcag cggccggagc
     1141 tcgccgtcgg agaggttccc ggacgtggcc gtcaccttga acgcgaccgt tcggacgccg
     1201 ggaggcgcga tcaggtggaa cgtctcgttc accccgccgc cggccgggac cgcgaccgcc
     1261 cggcgcgcgt cggcgaccgg gagaccgaag cgggccgtga ggtcctcgtt cgtctccggg
     1321 tcgaggacct ggagcacgag gctccccgcg agcgtgccct cggacgcgtt gttgacgacg
     1381 accttcagct cggcccggtc cccctcgcgc aggaagcgcg ggacgtaggg gcggaccatc
     1441 agctccttca cgctcttcgt ctcgcgcttg agcgaggcgc cgcggaggtc cttcgtcacg
     1501 gcgtggagcc agacgttcca ggaagtgacc gagtcgggga cggtgagctc gatcgacgcg
     1561 gagccatcgg ctccggtcag gagctgcggc gtgaagaacg cggtctcggc gaagttgctc
     1621 cggagggcgg gcgcctcggc cgccggcgcc acgccggcct ccgtccgcgc ctcgttcgcc
     1681 gcggcgtcct ccaccttcat ctcgaccgac ttcgccatct gcggggcggc ggcgggcgcg
     1741 ggggccggga cgccgtcccg ctccagggcc gcctcggaca ccatcgtcac gcccattccg
     1801 ccgcgcatcg cgaactttcg tccgcgcgcg ccgggcccgc cgatgccgta gcgatcgagc
     1861 tcgagcagcc gatcgggctc gaaggaaggg ccggacgggg tcggggcgaa gctgccccgg
     1921 acccactggg cccggcccgc gccgagcgtc gcacgcgtcc aggccgtccc cgtccgggag
     1981 gggtagaggg agaggatcga cgggggggtg tgtggagcga agacgtcgag gctccggtcg
     2041 tacatgtacg cgaggagctc ggccgcgccg gcctccgccg gcttccccga gggagacgtc
     2101 accttcacgc gccacgtctc gcgcgtcccc gggcggatcc ggtcgcggaa cgtcgcgaac
     2161 tccagcttca gctcgcggtc gtcccacggg acgtagaccg cggccgcctg gctcatccac
     2221 tggtggtcgc gcagggccgt gagcgtgacg ccgaagccgc cgcggtcggc ctccgtcacc
     2281 gggaactcga cgacggtcgg gtccttcccc gccgtcagga ccgtgcggga gaggagcttc
     2341 ccggcgcgga agcgctcgag gagcatctcc tgccccggca gccccgacgc cgcgaggagg
     2401 cgcgccgtcc cgccgaccac gaccgagccc gactcggcgg cgaggacgag cgggaccggc
     2461 ggcgccgcct tcccgccggc gacgatcacg tcgcgggagg tctcgtactt cgctccgaag
     2521 tcgtcgacgg tctcgtaccg gatccgccac gcccccgccg ggagggtgcc gaggtcgaga
     2581 gcggcgacgc cgtcggcgcc gtgccgtgcc tccccgcgcg ccttctcggc gccgtcgggg
     2641 aggtcgcgaa ggaccgcctc cacccggaac gccgtgtccc agcgcgggcg gagcgcgtct
     2701 ccgggtgtcc ggacggacgc tccctcccct tcctccgcct gctcgacgcg gcgcgggagg
     2761 tcggccggga gcggcgtctc ggccggcggg gcgagagcga tcagccgcca cgtcccgttc
     2821 ccggggcgag gcgcgccgtc gaggttcgtc cgcgtgatcc ttgccgacat cgcctccccg
     2881 gcccggccga atcccgacgg gaacttgacg tcggcctcga cggagacgaa gccgagccgg
     2941 acggcgcggg tcgcgctccg ggtctcgccc ccttcgtcga ggacgtcggc ctggacgacg
     3001 tagcgccagg tcgtctcctt cgagtccgcc ttcgtctcgt ccgcttccgg cgtgaaggag
     3061 acccggaacg tcccctccgc gtcgagcgtc gtctcgcccg cggcgacgac ctgcggcttc
     3121 gcgacggagc caaagcccca gaaagcccac caccagggtc ggagcgtctc gcgcgtgacg
     3181 cgccacttca ccgagccgtt cgtgacgggg aggccgaagt agtaccgcgc cacgccgacg
     3241 agcgacgcct tccggttgag gcgcatcggc tccttcggct cgaggaacgt cgcctcgaac
     3301 gtcggccgct tgtactcctc gacccgcacg gaggtgctcc cggagaggga gctctcgagg
     3361 cgccaggctc cgaggaggcg tcccgtgggg acgaccatct ccccggccgc ggtgccgaag
     3421 tcgttcgtcg agacggtccg cgtctcgacc cgctcgccgt tcgggtcgac gagccagacc
     3481 gtcaccggcg tccggtctgc gacgcggaaa cgggcgtcct cggcggaacc gcgataggcg
     3541 aggaccttcc agtggagcgt ctgccccggc cggtagatgc tccggtcggt gtagaggagc
     3601 gagtcgctcc ggagccccgg cttcgactcg cgatggaagc cgacctgctg gccgtcgaac
     3661 gtgaggtcgc ggcccttctt cgcgagcagg tggaacgcgc cgccctgctc ccgcagggcg
     3721 aacgagaccc gcccgtcgga gtccgaggcc ttcgcttcgg cgcgccggtg cccctcgcgc
     3781 cagtcgaacc gccagagcgt cacctcggct cccgcgaccg cccgccccgt cgcgcccgaa
     3841 aggacgcgcg ccgtaacggc gcccgtctcc ggatcggggc gcacggcgag gacgaggtcg
     3901 gagacgaaga ggttcgtccc gacgacgacg ttgtccgact
//
LOCUS       DAOTEZ010000414         3895 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_17280_length_3895_cov_4.284115, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000414 DAOTEZ010000000
VERSION     DAOTEZ010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17280_length_3895_cov_4.284115"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /locus_tag="P5164_16940"
     CDS             <1..1183
                     /locus_tag="P5164_16940"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRY45622.1"
                     /translation="AAVYRLRERRHPQAARLERLRERLPQTISTLLIGNNLVNIAAGS
                     IGTVLAIDALGARWGVLAATVVTTLLLLVFGEVTPKTLAARNADRVACFAAPVIELLM
                     TVFAPLAGLLSAIAAVLLRPFGGRATAASDVTEEDVKSLISLSHEHGQLEREEKEILH
                     AVLEFGDQPVVDAMVPRAKMVLLPADASFEKVEAIGRVWRYSRLPVYRDGPEDIVGIL
                     HLKDLFDVTDAEERTFSLSRYLRPAIFVPEQKRSGDLFREMRRRRFHMAIVVDELGAV
                     SGLVTLEDLVEQVLGDIDDEHDEDAALPVLDGASILIEGSYPVASLERDLGLPLGETE
                     METVAGFLLKKFGRIPRTGARTWLGDVEFVVERASPRAIERIRITRKPRAPEGAGDEK
                     V"
     gene            1185..>1406
                     /locus_tag="P5164_16945"
     CDS             1185..>1406
                     /locus_tag="P5164_16945"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRY45623.1"
                     /translation="MPKVVFLDQGVRGELPEGTTVLEAARHLGARLSSICGGAGTCST
                     CRVECVVNPQNLSPVEPLEVDGGEVLGVHD"
     assembly_gap    1407..1416
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1523..2197
                     /gene="trmB"
                     /locus_tag="P5164_16950"
     CDS             1523..2197
                     /gene="trmB"
                     /locus_tag="P5164_16950"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF014447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRY45624.1"
                     /translation="MRRALKRAGLSPAPKEFLESPGVPPPPRVALPGEAPGTESPIDW
                     AAFFGRVAPVELEIGSGRGRFLTDAAAERPGTSFLGAEIELEYTRIAQARAEKMGLSN
                     VRFARLDGKELVLRRVPGASLAAIHVFFPDPWPKKRHAKRRLFSPDFARAAAAALAPG
                     GLLRVATDNLPYFETIREVLDAEPGLEKVPGSESGWSSGTDYERKYEKEGRPMARGIW
                     RRPPRA"
     gene            complement(2205..2810)
                     /gene="pgsA"
                     /locus_tag="P5164_16955"
     CDS             complement(2205..2810)
                     /gene="pgsA"
                     /locus_tag="P5164_16955"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HRY45625.1"
                     /translation="MPNLPNALTLFRIFLVPLLVVILLTRMPAKEYVALGVFLVAALT
                     DFLDGWIARRYKKVTTLGMLLDPIADKILVSAALISLVDLQEADAWAVCIIVGREFAV
                     SGMRAIAAGEKITIAASKLGKWKMAAQVVGISLMILGARLDDIGLWRMTGRAALYVMT
                     VMALVSGVDYFRKYLPVLLARDAGAAEGAAPGADAPAAPKG"
     gene            2916..>3895
                     /locus_tag="P5164_16960"
     CDS             2916..>3895
                     /locus_tag="P5164_16960"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HRY45626.1"
                     /translation="MTSPIVFGTDGWRGRIGRDLTFDSLARVVDAVASWTASPGNVDP
                     GDASTLPLVHDTRFLSRELAAESAALLARRGFRVLLTDRPVPTPCASWHVKSRALRGG
                     VAITASHNPPEWNGVKYKSWFGGSATAETYASIAACAGRPLPERPGGTVEKVDLLSSY
                     SDAIAACVDLDAIRRSGLRVLWDSMHGASGDLLARILGDGNATRVTTIRSEVNPSFGG
                     VHPEPIPEHLGASVSRLAAEPFDVALASDGDGDRLGVLAPDGTFVTPHRVLALLAESL
                     ARRGRIAGGIAKTFSTSLLVDRVAARLGVPLHVTPIGFKWIAEKMLSGEVG"
BASE COUNT          543 a         1458 c         1334 g          550 t
ORIGIN      
        1 ggccgccgtc taccggctga gggagaggcg gcacccccag gcggcccgcc tcgagcggct
       61 ccgcgagcgg cttccccaga cgatctcgac gctcctgatc gggaacaacc tcgtgaacat
      121 cgccgccggc tcgatcggga ccgtcctcgc gatcgacgcc ctcggcgcgc gctggggagt
      181 cctcgccgcc acggtcgtca cgacgctcct gctcctcgtc ttcggcgagg tgacgccgaa
      241 gacgctggcg gcgcgcaatg ccgaccgcgt ggcctgcttc gccgcccccg tcatcgagct
      301 cctgatgacg gtcttcgcgc cgctcgccgg gctcctctcg gccatcgccg ccgtccttct
      361 ccgccccttc ggaggacggg cgaccgccgc ctcggacgtc acggaggagg acgtgaagag
      421 cctcatctcg ctctctcacg agcacggcca gctcgaacgc gaggagaagg agatcctcca
      481 cgcggtcctg gagttcggcg accagcccgt cgtcgacgcg atggtgccgc gcgcgaagat
      541 ggtcctcctc ccggccgacg ccagcttcga gaaggtcgag gcgatcggcc gagtctggcg
      601 ctacagccgg ctgccggtct atcgcgacgg ccccgaggac atcgtcggca tcctccacct
      661 gaaggacctc ttcgacgtca cggacgccga ggagcgcacg ttcagcctct cccggtacct
      721 tcgcccggcg atcttcgtcc cggagcagaa gcggtccggc gacctcttcc gggagatgcg
      781 gcggcgccgg ttccacatgg cgatcgtcgt cgacgagctc ggcgcggtct cgggcctcgt
      841 cacgctcgag gacctggtcg agcaggtcct cggcgacatc gacgacgagc acgacgagga
      901 cgccgccctc ccggtcctcg acggggcctc gattctgatc gagggctcct atcccgtcgc
      961 ctcgctcgag agagacctcg gcctcccgct cggggagacc gagatggaga cggtggcggg
     1021 cttcctcctg aagaagttcg gccggatccc ccgcacgggc gcccggacct ggctcggcga
     1081 cgtggagttc gtcgtcgagc gcgcctcgcc gcgcgcgatc gagcggatcc ggatcacgag
     1141 aaagccccgc gctcccgaag gagccgggga cgagaaggtc tgacatgccg aaggtcgtct
     1201 tcctcgacca gggcgtgcgc ggggagcttc ccgaggggac gacggtcctc gaagcggccc
     1261 ggcacctcgg agcgcgcctc tcctcgatct gcgggggcgc cgggacctgc tcgacgtgcc
     1321 gcgtcgagtg cgtcgtgaac ccccagaacc tctcccccgt cgagccgctg gaggtcgacg
     1381 ggggagaggt tctgggggtt cacgacnnnn nnnnnnacat ggggtccggc gtgcgcctcg
     1441 gctgccaggc gcggatcctc ggagacgtcg gcctgcggac cgtccggatc ccgaaggcgg
     1501 tgctcgattg agccggccgg ccgtgcgccg cgcgctcaag cgcgccggcc tctcgcccgc
     1561 gccgaaggag ttcctcgagt ctcccggcgt tccgccgccg ccccgcgtgg cgctccccgg
     1621 cgaggccccg ggcaccgaaa gcccgatcga ctgggcggcg ttcttcggcc gcgtcgcgcc
     1681 ggtcgagctc gagatcggga gcggccgggg acgcttcctg accgacgcgg ccgcggagag
     1741 acccgggacg tcgttcctcg gggcggagat cgagctcgaa tacacgcgga tcgcgcaggc
     1801 gcgcgcggag aagatgggcc tttccaacgt gaggttcgcg cggctcgacg ggaaggagct
     1861 cgtcctgcgg cgggtgcccg gggcctccct cgcggcgatc cacgtcttct tccccgaccc
     1921 ctggccgaag aagcggcacg cgaaaaggcg gctcttctcg cccgacttcg cgcgcgcggc
     1981 ggccgcagcg ctcgccccgg gcgggctcct ccgcgtggcg acggacaacc tcccctactt
     2041 cgagacgatc cgggaggtcc tcgacgccga gccggggctc gagaaggtcc ccgggagcga
     2101 gagcgggtgg agctccggaa ccgactacga gcggaagtac gagaaggaag ggcgcccgat
     2161 ggcgcgcggg atctggcgcc ggccgccgcg agcctgagac gccgtcagcc cttcggggcc
     2221 gccggcgcgt ccgctccggg agccgcccct tccgccgctc cggcgtcccg cgcgagcagg
     2281 acgggcaggt acttgcggaa gtagtcgacg cccgacacga gcgccatgac cgtcatgacg
     2341 tagagcgccg ctcgccccgt catccgccag agaccgatgt cgtcgagccg cgccccgagg
     2401 atcatgagcg agatcccgac gacctgggcg gccatcttcc acttcccgag cttcgacgcg
     2461 gcgatcgtga tcttctcgcc cgcggcgatc gcgcgcatcc ccgaaaccgc gaactccctc
     2521 cccacgatga tgcagacggc ccacgcgtcg gcctcctgga ggtcgacgag ggagatgagc
     2581 gcggccgaga cgaggatctt gtcggcgatc ggatcgagca gcatcccgag cgtcgtgacc
     2641 ttcttgtacc ttcgggcgat ccagccgtcc aggaagtccg tgagcgcggc gacgaggaag
     2701 acgccgagcg ccacgtattc cttggccggc atccgcgtca ggaggatgac gacgaggagc
     2761 ggaacgagga agatgcggaa gagcgtgagc gcgttcggga ggttcggcac cgctgcgcat
     2821 tctaggagac cgctcgggca aatgctcctc ccggcccgag catgcgcggg ccgggccttg
     2881 cactccggtc gcgtcccgcc gagaatcggc gtcccatgac gtcgcccatc gtcttcggga
     2941 cggatggctg gcgcggccgg atcggacgcg acctgacctt cgactccctc gcccgcgtcg
     3001 tcgacgcggt cgcctcctgg accgcatcgc ccgggaacgt cgatcccggc gacgcttcca
     3061 cgctcccgct cgtccacgac acacgcttcc tctcccgcga gctcgccgcc gagagcgcgg
     3121 cgctcctcgc gcggcgcggc tttcgcgtcc tcctgaccga ccgccccgtc cccacgccgt
     3181 gcgcctcgtg gcacgtgaag tcccgcgcgc tccggggggg cgtggcgatc accgcctccc
     3241 acaacccgcc cgagtggaac ggcgtcaagt acaagtcgtg gttcggaggg agcgcgacgg
     3301 cggagaccta cgcgtccatc gccgcgtgcg ccggccgccc gctccccgaa cgccccggcg
     3361 gaacagtcga gaaagtcgac ctcctttcct cgtactcgga cgcgatcgcc gcctgcgtcg
     3421 acctcgacgc gatccgccga tcgggcctcc gggtcctctg ggactcgatg cacggcgcct
     3481 cgggcgacct cctcgcccgg atcctcggcg acgggaacgc cacccgcgtc acgacgatcc
     3541 ggtccgaggt gaatccgtcc ttcggcggcg tccaccccga gccgatcccc gagcacctcg
     3601 gcgcctccgt ctcccgcctc gccgcggagc cgttcgacgt cgccctcgcc agcgacgggg
     3661 acggagaccg cctgggcgtc ctcgccccgg acgggacgtt cgtcacgccc caccgcgtcc
     3721 tcgccctcct cgcggagagc ctcgcgcggc ggggccggat cgcgggcggg atcgcgaaga
     3781 cgttctcgac ctccctcctc gtcgaccgcg tcgccgcgcg gctcggcgtc ccgctccacg
     3841 tcacgccgat cggcttcaag tggatcgccg agaagatgct ctcgggcgag gtcgg
//
LOCUS       DAOTEZ010000415         3885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_17338_length_3885_cov_5.881723, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000415 DAOTEZ010000000
VERSION     DAOTEZ010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17338_length_3885_cov_5.881723"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1743
                     /gene="rnr"
                     /locus_tag="P5164_16965"
     CDS             <1..1743
                     /gene="rnr"
                     /locus_tag="P5164_16965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HRY45627.1"
                     /translation="VTELIGFPGEEGVDVEVVARKWGIPREFPKAVLDDAKRAIRDVG
                     EAERASRRDLTCDVVVTIDGESARDFDDAISVEELPGGGFRLGVHIADVSHYVRPGTA
                     LDAEAFERGTSVYFPDRAVPMLPERLSTDLCSLRPHEERRTVTALLTLDEKGETRKAE
                     FFRSVIRSSARLIYTDVAAFFSGEEPGEEKLPASVRPMLLAARKAAFALRWMRRGRGA
                     LDFDLPDADLILGEKGAVVDIVRAVRNEAHRLVEEFMLAANEAVAKHLLFVPTPAMYR
                     VHDRPDERKLADLRAVLEPLGYEIPAEDDAVSPGVFQSILDQAEGRPEERFVSDLVLR
                     AQKKALYAPECRGHYALAAPYYAHFTSPIRRYPDLVVHRALCEWIASGRPPATAEAES
                     RERWLSDASAQCSERERRAESAEREAQSWKKFVFLSKKVGEEFEAYVSAVVPFGLFIT
                     LEDVYADGLLPMDALGDDWYRYEDVEHRLVGESSGRVFRLGDHLRVKLVRADFDKRLL
                     DFQLHGVPPARRDDRVAPPVWTPAPAPRRFAGGEPKGAPKERAGAPRGGRKAPARGGP
                     PTKTSRGAPRGRRK"
     gene            1740..3077
                     /gene="der"
                     /locus_tag="P5164_16970"
     CDS             1740..3077
                     /gene="der"
                     /locus_tag="P5164_16970"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRY45628.1"
                     /translation="MTEAARGARIWSVALVGRPNVGKSALFNRLSGTRKALVHDRPGM
                     TRDALALDLATETGRRWRLVDTGGLDLDAAGGFAAWTSEKALEAVVDADLLVLVLDGA
                     DGVLPEDERIATRLRALGKPVVVAWNKVDRKEAEAGLEQAYRLGFEDVFGVSAVHGLG
                     SDELLEALETRLPEDLDPETRAEGLPIAIVGRPNVGKSSIVNRIVGAERVMVSEIAGT
                     TRNPVDVELVRGGKSYRLVDTAGIRRKGKTSDSAELLSVVAARKAMERARIAVVIFDA
                     SEGITSQDATIAGYAEEARRSLVLVANKWDLLADDPGAQKTLREDVRRRFVFTRGAAF
                     LTVSAKTGAGVSKILPEADAVARRSTLKIPTGELNRVLKKAFEAQLPKGRSGRELKIR
                     YAVQIASEPPLIRLFSDRDEPLHFTFERFLQNRLREKWDLAGVPIRFVVRKGE"
     gene            3124..3783
                     /locus_tag="P5164_16975"
     CDS             3124..3783
                     /locus_tag="P5164_16975"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRY45629.1"
                     /translation="MSETKSASEARYKVAEVCRLADVQPYVLRYWESEFPVLAAPKGA
                     SGPRLYSALELKIIERIKKLLYDEGYTIAGAKKRLEAELKEGEPPAAEPEPLVLTAPA
                     PEPSESTGTRRRRTKSAPPPEPEPPAASPEPVLELSEAPILATHAVAPSPEPSIPLEI
                     DESPAAAVDVDDAPPVKTAPVVRKIDPAPAPMAQSPDPRVAMALAELKEIASLLSRED
                     A"
     gene            3808..3884
                     /locus_tag="P5164_16980"
     tRNA            3808..3884
                     /locus_tag="P5164_16980"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3842..3844,aa:Pro,seq:ggg)
BASE COUNT          550 a         1514 c         1310 g          511 t
ORIGIN      
        1 gtgaccgagc tgatcggctt cccgggcgag gaaggggtcg acgtcgaggt cgtcgcccgc
       61 aagtggggca tcccccgcga gttcccgaag gcggtcctcg acgacgcgaa gcgggcgatc
      121 cgcgacgtcg gcgaggccga gcgcgcttcg cgccgcgacc tgacgtgcga cgtcgtcgtg
      181 acgatcgacg gcgagtcggc ccgcgacttc gacgacgcga tctccgtcga ggagcttccg
      241 ggagggggct tccggctcgg cgtccacatc gccgacgtct cccactacgt gaggccgggc
      301 accgccctcg acgccgaggc gttcgagcgc ggcacctccg tctacttccc cgaccgcgcc
      361 gtcccgatgc tccccgagcg cctctcgacc gacctctgct ccctccgccc gcacgaggag
      421 cggcggacgg tgacggcgct cctgacgctc gacgagaagg gcgagacccg gaaggcggag
      481 ttcttccgga gcgtcatccg ctcgagcgcg cgcctcatct acaccgacgt cgcggccttc
      541 ttctccggcg aggagccggg ggaggagaag ctccccgcga gcgtgaggcc gatgctcctg
      601 gccgcccgca aggcggcgtt cgccctccgc tggatgcgcc gcggccgcgg cgcgctcgac
      661 ttcgacctcc ccgacgccga cctgatcctc ggcgagaagg gggccgtcgt cgacatcgtc
      721 cgggccgtcc gaaacgaggc gcaccggctc gtcgaggagt tcatgctcgc cgccaacgag
      781 gccgtggcga agcacctcct cttcgtcccg accccggcga tgtaccgggt ccacgaccgc
      841 cccgacgagc gcaagctcgc cgacctgcgc gccgtcctcg agccgctcgg ctacgagatc
      901 ccggccgagg acgacgccgt cagcccgggc gtcttccagt cgatcctcga ccaggccgag
      961 ggcaggcccg aggagcggtt cgtctccgac ctcgtcctcc gcgcgcagaa gaaggcgctc
     1021 tacgcgcccg agtgccgcgg ccactacgcg ctcgccgctc cgtactacgc gcacttcacc
     1081 tcgccgatcc ggcgctaccc cgacctcgtc gtccaccgcg ccctctgcga gtggatcgcg
     1141 tccgggcgac ccccggccac ggccgaggcc gagagccgcg agcgctggct ctccgacgcc
     1201 tccgcccagt gctccgagcg cgagcgtcgc gccgagtcgg ccgagcgcga ggcgcagtcg
     1261 tggaagaagt tcgtcttcct ctcgaagaag gtcggcgagg agttcgaggc ctacgtctcc
     1321 gccgtcgtcc cgttcggcct cttcatcacc ctcgaggacg tctacgccga cggcctcctc
     1381 ccgatggacg ccctcggcga cgactggtac cgctacgagg acgtcgagca ccgcctcgtg
     1441 ggcgagtcga gcgggcgcgt cttccgcctc ggcgaccacc tgcgcgtcaa gctcgtccgc
     1501 gccgacttcg acaagcgcct cctcgacttc cagctccacg gcgtcccgcc ggcccggcgc
     1561 gacgaccgcg tcgcgccccc cgtctggacg ccggcgcccg cgccgcggcg cttcgccggc
     1621 ggcgagccga agggggcgcc gaaggagcgt gcgggcgccc cacgcggcgg ccggaaggcc
     1681 ccggcccgcg ggggcccgcc gacgaagacc tcccgcggcg ccccgagggg gaggcggaag
     1741 tgacggaggc ggcgcgcggc gcgcgcatct ggtcggtcgc cctcgtcggc cgcccgaacg
     1801 tcggcaagtc ggccctcttc aatcggctct ccggcacccg gaaggccctc gtccacgacc
     1861 gtcccggcat gacccgcgac gccctcgcgc tcgacctcgc gaccgagacc ggccgccgct
     1921 ggcggctcgt cgacaccggc ggactcgacc tcgacgcggc cggcggcttc gccgcctgga
     1981 cgagcgagaa ggcgctcgag gccgtcgtcg acgccgacct cctcgtcctc gtcctcgacg
     2041 gcgccgacgg cgtcctcccg gaggacgagc ggatcgccac ccgcctgcgg gcgctcggca
     2101 agccggtcgt cgtcgcctgg aacaaggtcg accgcaagga ggccgaggcg ggcctcgagc
     2161 aggcctaccg cctcgggttc gaggacgtct tcggcgtctc ggccgtccac gggctcggca
     2221 gcgacgagct cctcgaggcg ctcgagacgc gcctccccga ggacctcgat cccgagaccc
     2281 gcgccgaggg gctccccatc gccatcgtcg gccggccgaa cgtcggcaag tcctcgatcg
     2341 tcaaccggat cgtcggcgcc gagcgggtca tggtctcgga gatcgccggc accacccgga
     2401 atcccgtcga cgtggagctc gtccgcggcg gcaagagcta ccgcctcgtc gacaccgcgg
     2461 gcatccggcg caaggggaag acgtccgact cggccgagct cctctccgtc gtcgccgcgc
     2521 gaaaggcgat ggagcgcgcg cggatcgccg tcgtcatctt cgacgcctcc gaggggatca
     2581 cctcccagga cgccaccatc gccggctacg cggaggaggc gcgccgcagc ctcgtcctcg
     2641 tcgcgaacaa gtgggacctc ctcgccgacg accccggcgc ccagaagacc cttcgcgagg
     2701 acgtccgccg ccgcttcgtc ttcacgcgcg gcgccgcctt tctcaccgtc tccgcgaaga
     2761 ccggcgcggg ggtctcgaag atcctccccg aggccgacgc cgtcgcccgc cgctccacgc
     2821 tgaagatccc gacgggggag ctgaaccggg tcctgaagaa ggccttcgag gcccagctcc
     2881 cgaaggggcg ctccgggcgc gagctgaaga tccgctacgc cgtccagatc gcgtcggagc
     2941 cgcccctcat ccggctcttc tccgaccggg acgagccgct ccacttcacg ttcgagcgct
     3001 tcctccagaa ccggctgcgg gagaagtggg acctcgccgg cgttccgatc cggttcgtcg
     3061 tccgcaaggg cgagtaggcg tttgctttag actcgggacc gcttccagga gggaccgcac
     3121 ggcgtgagcg agaccaagtc ggcttcggag gcccgctaca aggtggcgga ggtctgccgt
     3181 ctggcggacg tccagcccta cgtcctccgc tactgggaga gcgagttccc cgtcctcgcg
     3241 gccccgaaag gggcgagcgg cccgcgcctc tacagcgcgc tcgagctgaa gatcatcgag
     3301 cggatcaaga agctcctcta cgacgagggc tacaccatcg ccggcgccaa gaagcgcctc
     3361 gaagccgagc tgaaggaagg ggagcccccg gccgccgagc cggagccgct cgtcctcacc
     3421 gccccggcgc cggagccgag cgagtcgacg gggacgcgcc ggaggaggac gaagtccgcg
     3481 ccgccgcccg agcccgagcc gccggccgcc tctcccgagc ccgtcctcga gctctccgag
     3541 gcgccgatcc tcgccacgca cgcggtcgcc ccgtcgcccg agccgtcgat ccccctcgag
     3601 atcgacgaga gcccggcggc cgccgtcgac gtcgacgacg ctccgcccgt aaagaccgcg
     3661 cccgtcgtcc gcaagattga ccctgccccg gctccgatgg cacaatcccc ggacccccgg
     3721 gtggcgatgg ccctcgcgga actgaaggag atcgcaagcc tcctttcccg cgaagacgcc
     3781 tgatcctccc gtcctttcgt ccagggtcgg gacgtggcgc agcctggtag cgtacttgaa
     3841 tggggttcaa ggggtcgcgg gttcgaatcc cgccgtcccg accat
//
LOCUS       DAOTEZ010000416         3874 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_17404_length_3874_cov_4.757266, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000416 DAOTEZ010000000
VERSION     DAOTEZ010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17404_length_3874_cov_4.757266"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="P5164_16985"
     CDS             <1..248
                     /locus_tag="P5164_16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRY45630.1"
                     /translation="KNVFEGIQVPAGEIYSYTEGANGELGYSIVSDGSGRPYRVKVRP
                     PCFPLFAAFEKIIVGGMMADAVATLGTLNIIAGELDR"
     gene            259..936
                     /locus_tag="P5164_16990"
     CDS             259..936
                     /locus_tag="P5164_16990"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HRY45631.1"
                     /translation="MPGKVVILEPPAETLAVRLYLPLLAGMALTFRKFVANVLSRKAR
                     PTIEYPEERRDYSDRFRGRHILKAREDGTLKCVACYMCATACPADCIHIEAGEHPNLA
                     YEKYPTVFEIDLLRCVMCGFCVDACPKDAIWMTKDYELSFFTRKDAVYGIPELTEKPS
                     DTAPDGPGYGYRPYYGESPAKKAGQFEPSGLAVLPMTLARNAAINRMPPPPPKPPAPA
                     PAPAKPA"
     gene            complement(1012..1146)
                     /locus_tag="P5164_16995"
     CDS             complement(1012..1146)
                     /locus_tag="P5164_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45632.1"
                     /translation="MLKLIPPDPRVRPEDTRSRAGGDWQPVDATGRPYQLRLGARLVW
                     "
     gene            complement(1520..2317)
                     /locus_tag="P5164_17000"
     CDS             complement(1520..2317)
                     /locus_tag="P5164_17000"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY45633.1"
                     /translation="MNAATPPAAPPLLDLEGRIALVTGASRGIGAATARLLGRCGARV
                     AIHYGHSRESAEAVAEGIALAGGPPAALFQAELASDDERRRLHREVASTLGDPLLLVH
                     NAGIYVRNPFEETDDAAFLSRWRTTQAVNLEAAAHLTLLALPAMRAARFGRVVMVASR
                     AAFRAETDAASYAVSKAGMVSLARCLARNEGKHGITANAICPGWVDTAMARPDVAARK
                     AEIEGEVPLGRIATPEDCARAILFYLSPLASYANGTALGLNGGSFLH"
     gene            complement(2349..3209)
                     /locus_tag="P5164_17005"
     CDS             complement(2349..3209)
                     /locus_tag="P5164_17005"
                     /inference="COORDINATES: protein motif:HMM:NF015586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45634.1"
                     /translation="MSLWDVGVDLGATLAKAVAVPSGRPLEAFESFVAPAGDERLLDA
                     FLLRHSLRHLAATGGGATALADRLGEGRPVTIVDEFAAWGAGEPLLTRRAGLELHSPH
                     LLVSLGTGTSILRMGARGSVARVGGTALGGGTLRGLGRLLLGTDDHERLVALAAAGDR
                     RRVDLLVGDLYRPGGIALMPDLTAANFGKAHEPRAVDVAHALLGLVGENVALLAGQIA
                     VALATPSGGGPPRRRPDVLYAGSTLRNNPLLVEILGNVTALVGARASFLPFGEFTGAL
                     GALARARAGT"
     gene            3363..>3874
                     /locus_tag="P5164_17010"
     CDS             3363..>3874
                     /locus_tag="P5164_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRY45635.1"
                     /translation="MHDSVLDAISDSFGDNAAFALELYAQYRLNPGTVGDDWRRTFEK
                     LEKAARVAAASGPGTAPEAAAPAPATPAPAVVSRPAPSLRPGETLQPLVGGSATIARN
                     MDASLSVPTATSQRVIPVKAMEENRRILNLHREAVRQSKVSFTHLVAWAVVRALEKHP
                     GMNDAYAEAEG"
BASE COUNT          552 a         1370 c         1478 g          474 t
ORIGIN      
        1 tcaagaacgt cttcgagggg atccaggtcc ccgcgggaga gatctactcg tacacggagg
       61 gggcgaacgg cgagctcggc tactcgatcg tctccgacgg gagcggacgc ccttaccgcg
      121 tgaaggtgcg ccccccctgc ttcccgctct tcgcggcgtt cgagaagatc attgtcggcg
      181 gcatgatggc cgacgccgtc gccacgctcg gcacgctcaa cattatcgcc ggcgagctgg
      241 accggtagga gagacgcgat gcctggaaag gtcgtcatcc tcgagccccc ggccgagacc
      301 ctcgccgtcc ggctctacct tccgctcctc gcgggcatgg ccctgacgtt ccggaagttc
      361 gtcgcgaacg tcctctcgcg caaggcgcgc ccgacgatcg agtaccccga ggagcgccgc
      421 gactactccg atcgcttccg cggccggcac atcctgaagg cccgcgagga cgggacgctg
      481 aagtgcgtcg cctgctacat gtgcgcgacg gcctgcccgg cggactgcat ccacatcgag
      541 gcgggggagc acccgaacct cgcctacgag aagtacccga ccgtcttcga gatcgacctc
      601 ctccggtgcg tgatgtgcgg cttctgcgtc gacgcctgcc cgaaggacgc gatctggatg
      661 acgaaggact acgagctctc gttcttcacg aggaaggacg ccgtctacgg catccccgag
      721 ctgaccgaga agccgtcgga cacggcgccg gacgggccgg gctacggata tcgcccctac
      781 tacggcgaga gcccggcgaa gaaggccggc cagttcgagc cgtcgggcct cgccgtcctg
      841 ccgatgacgc tcgcgcggaa cgcggcgatc aaccggatgc cgccccctcc gccgaagccg
      901 ccggccccgg cgcccgcccc ggccaagccg gcgtagcggg cgaacgttct cctcgaaaaa
      961 ccgaacgccc cgggaccttc ccggggcgtt cgcgcttcgg ggcgtccccg cctaccagac
     1021 gaggcgggcg ccgaggcgga gctggtaggg gcgtccggtc gcgtcgaccg gctgccagtc
     1081 gccgccggcc cggctccgag tgtcttccgg gcggacccgc gggtcgggcg ggatcagctt
     1141 cagcaccgcc tcgttcacct cgatggcgaa acggagccga ggctcgccgg acgggacggg
     1201 tcccccgagc gtcaggacgg agtccgggtc cccggggagg cggatgaggc tctcgtcgct
     1261 gaagacgccg agctcggggg ggagaggcgt ctaacggaag aggccggtgg ggaggacgag
     1321 ccggaagccg gaaccctcgg cggcgcgcgc cgtcactccg ggatcggcgg ccgcgaggcc
     1381 ggcgaggagg gacgctacca gcaccagggc tctcccgcag ggaacgagca cgagggcatt
     1441 ctctcccgaa agcgtgctcg gccgcaccaa ctatctcgcc ggccccgttc ggagggaatc
     1501 cggaagccga gcagccgggt cagtgaagga acgagccccc gttcagcccg agcgccgtcc
     1561 cgttcgcata ggaagcgagg ggggagaggt agaagaggat cgcacgcgcg cagtcttcgg
     1621 gcgtcgcgat ccgcccgagc gggacctctc cttcgatctc ggccttgcgg gcggcgacgt
     1681 ccgggcgcgc catcgccgtg tcgacccacc cggggcagat cgcgttcgcc gtgatcccgt
     1741 gcttcccttc gttccgggcg aggcagcggg cgaggctcac catccccgcc ttcgagacgg
     1801 cgtagctcgc ggcgtcggtc tcggcacgga acgcggcgcg ggaggcgacc atcacgacgc
     1861 gcccgaagcg ggcggcgcgc atcgcgggaa gcgccaggag cgtgaggtgc gcggccgcct
     1921 cgaggttgac ggcctgcgtc gtccgccagc gcgagaggaa cgcggcgtcg tcggtctcct
     1981 cgaacgggtt ccggacgtag atgccggcgt tgtggacgag gaggagcggg tcgccgagcg
     2041 tgctcgcgac ctcgcgatgc aggcgcctcc gctcgtcgtc gctcgcgagc tcggcctgga
     2101 agagcgcggc gggcggcccg ccggcgagcg cgatcccctc cgcgacggcc tcggcgcttt
     2161 cgcggctgtg gccgtagtgg atcgcgacgc gcgccccgca gcgtccgagg aggcgggccg
     2221 tggccgcgcc gatgccgcgg gaagcgccag tgacgagggc gatccggcct tcgaggtcga
     2281 ggagaggagg cgcggcaggg ggcgtcgcgg cgttcatccc gggagtttcg cacccgcggg
     2341 gccgggcgtc aggttcccgc gcgcgcccgg gcgagggcgc cgagcgctcc cgtgaactcc
     2401 ccgaacggca ggaacgaggc gcgggcgccg acgagggccg tgacgttccc gaggatctcg
     2461 acgaggaggg ggttgttccg gagggtcgag cccgcgtaca ggacgtcggg gcggcggcgg
     2521 ggcggtccgc cgcccgacgg cgtcgcgagg gcgacggcga tctggcccgc gaggagcgcc
     2581 acgttctcgc cgacgaggcc gaggagggcg tgggcgacgt cgacggcgcg cggctcgtgc
     2641 gccttgccga agttggcggc ggtcaggtcg ggcatcaggg cgatcccgcc cgggcggtag
     2701 aggtcgccga cgaggaggtc gacgcgccgc cggtcgccgg cggccgcgag cgcgacgagc
     2761 cgttcgtggt cgtccgtgcc gaggaggagg cggccgagtc cgcggagcgt gccgccgccg
     2821 agggccgttc cgccgacgcg ggccacgctc ccgcgggcgc ccatccggag gatcgaggtg
     2881 ccggtcccga ggctcacgag gaggtggggc gagtggagct cgaggccggc ccgtcgcgtc
     2941 aggagcggct cgccggcgcc ccaggcggcg aactcgtcga cgatcgtcac cggccggccc
     3001 tctccgaggc ggtcggcgag cgccgtggcg ccgcccccgg tcgccgcgag gtggcggagg
     3061 gagtggcgca ggaggaaggc atcgaggagg cgctcgtcgc cggccggcgc gacgaacgac
     3121 tcgaaggcct cgagcgggcg gccggacgga accgccacgg ccttggcgag ggtcgcgccg
     3181 aggtcgacgc cgacgtccca gaggctcatc gcggagcatt gtcgcccgtt cggggcgcgg
     3241 acggagcggg cgtcggcgtt cgggggtcgg ggtaacatgc ccgcgctgcc ggcagcccgg
     3301 ccgacagagg ggccgatgac gcccgccggg cgcagacgac ccggccgaca gggagacccc
     3361 gaatgcacga ctccgtcctc gacgccatct ccgattcgtt cggcgacaac gccgcgttcg
     3421 ctctcgagct ctacgcccag taccggctga acccggggac ggtcggtgac gactggcggc
     3481 gtacgttcga gaagctcgag aaggccgcgc gcgtcgccgc ggcctccggg cccggaacgg
     3541 cgcccgaggc cgcggcgccg gcgcccgcga caccggcgcc cgccgtcgtc tcccgccccg
     3601 cgccgtcgct ccgcccgggc gagacgctgc agccgctcgt cggcgggtcc gccacgatcg
     3661 cgcgcaacat ggacgcctcc ctctccgtcc cgaccgcgac gtctcagcgc gtgatcccgg
     3721 tcaaggcgat ggaggagaac cggcgcatcc tgaacctcca ccgcgaggcg gtccggcagt
     3781 cgaaggtctc cttcacgcac ctcgtcgcgt gggccgtcgt ccgggcgctc gagaagcacc
     3841 ccgggatgaa cgacgcgtac gccgaggcgg aggg
//
LOCUS       DAOTEZ010000417         3864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_17470_length_3864_cov_4.541612, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000417 DAOTEZ010000000
VERSION     DAOTEZ010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17470_length_3864_cov_4.541612"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="P5164_17015"
     CDS             <1..349
                     /locus_tag="P5164_17015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="amine oxidase"
                     /protein_id="HRY45636.1"
                     /translation="PKADVFHSIVTRDSVPDADWRAFSFHFRPGLARDERLARAAQVL
                     GLTRGDLEEASERRTVLPSPVLGHETVVAEVDRLSAGKPLAILGNWFAGLSIEDCVDR
                     SRQEWARLGALTG"
     gene            470..3058
                     /locus_tag="P5164_17020"
     CDS             470..3058
                     /locus_tag="P5164_17020"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRY45637.1"
                     /translation="MGEVYRARDSKLRRDVAVKVLPATLAADAAALARFEREALAVAA
                     LSHPNILSIYDFGSAGGVTYAVMELVEGETLRSLLGDRPVPVPRAVDLALQIARGLAA
                     AHGKGIIHRDLKPENVMVSTDGHVKILDFGIAKQTEPRRVDETSAETMTERTEPGAMV
                     GTLSYMSPEQVRGHPVDGRSDLFSLGAVLYEMLSGKRAFRKESAAETTAAILLLEPPE
                     PAASLPGVSPALARVVERCLEKEPEKRFASARELIAALAEAQTGTATGVVTGAADLTG
                     TRKGARLPLVAGLVVSVVAALSAAWLARRPGPDTDESASSRRIAVLPFENVGPAEDDY
                     LADGIADEVRGKLTSVPGLEVIARGSSTPYRRTPKTPGTVARELDVRYLLTATVRWER
                     AKERSRVLVSPELVEVTGRGEPVSRWQQPFEAELTDVFAVQAEIAAQVVRELGIALGA
                     GTEKRLLERPTRDVAAYDAFLRGEAASASFGVTDPASLRRAVALYEEAVALDPAFAQA
                     WARLARACAHLHTNSVPTPAMLDRARSAAERALALAPRSPEAHLAQAAYVGLAFADPN
                     LELDLLEKARRLAPDDPEVLTAAALVEQSLGRWDDTLAHLKRAERLDPRSVLTKRRLA
                     LAYLLLRRQSEALETIELGRKIAPSSLVLVELKVVALLALGDAPGARAVLDTVPSEVS
                     PDRLLAHLAGFQDLGWLFDERRLRLLLDLPPAAFDGDRGTWGLRLSRASARAGNDARA
                     RALAGEARRAFEEQLRASPDDPERLASLGLALAYLGRAAEAARAGERAVALVPVEKDA
                     YRGPLLLHQLARIRAATGRNEEAIDALERLLAVPYTLTPAWLRVDPTFDGLRANPRFR
                     KLAAAG"
     gene            complement(3091..>3864)
                     /locus_tag="P5164_17025"
     CDS             complement(3091..>3864)
                     /locus_tag="P5164_17025"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRY45638.1"
                     /translation="PPPPPPKPELIPQEIPLSILFEDDHLLVLDKPPGLVVHPAVGHR
                     DGTLVNALLFRSKDWAGEADRPGLVHRLDKDTSGVLVVAKTEAAHAGMAKAMKARTLV
                     KEYLAVVYGRTAVAKGKVDLKILRDPHDRKRMSTSKTEGRDSTTLYERLAESTGERSG
                     LSLLLCTLVTGRTHQIRVHLKAIHHPIVGDATYGSPRWKGIADPSLQAACRDFPRQAL
                     HARRLAFDHPVTRARVDAIAPIPADISALLEAAGLAAPG"
BASE COUNT          495 a         1394 c         1464 g          511 t
ORIGIN      
        1 gccgaaagca gacgtctttc actcgatcgt gacgcgcgac tcggtgcccg acgccgactg
       61 gcgcgcgttc tcgttccact tcaggccggg gctcgcgagg gacgaacgcc tcgcgagggc
      121 tgcgcaggtg ctcgggctga cgcgcggcga cctcgaggag gcgtcggagc gacggacggt
      181 cctgccgtcg cccgtcctcg ggcacgagac ggtcgtcgcg gaggtcgacc gcctctcggc
      241 cggcaagccg ctcgcgatcc tcgggaactg gttcgccggg ctctcgatcg aggactgcgt
      301 ggaccgctcg cgacaggagt gggcgcggct cggggcgctc acggggtagc cccgcaaccg
      361 tagggaacgg tccggttccc ccggtatcat ggcggacgca tcccatgcat ctgccgcccg
      421 ggaccacgct cggcacgtac gagatcggcc cgcctctggg cgccggcgga tgggggaggt
      481 ctaccgggcc cgcgactcga agctccggcg ggacgtggcg gtgaaggtcc tgccggcgac
      541 cctcgcggcc gacgccgcgg cgctcgcgcg attcgaacgc gaagcgctcg cggtcgcggc
      601 gctctcgcac ccgaacatcc tgtcgatcta cgacttcggg agcgcgggcg gggtgacata
      661 cgccgtcatg gagctcgtcg agggggagac gctccggagc ctcctgggcg acaggcccgt
      721 tcccgttccg cgggcggtcg acctcgccct ccagatcgcg cgggggctcg cggccgcgca
      781 cgggaagggg atcatccacc gcgacctgaa gcccgagaac gtgatggtct cgacggacgg
      841 gcacgtgaag atcctcgact tcgggatcgc gaagcagacc gagccgcgcc gggtcgacga
      901 gacgagcgcc gagacgatga ccgagcggac cgagcccggc gcgatggtcg ggacgctcag
      961 ctacatgtcg cccgagcagg tccggggaca cccggtggac gggcggagcg acctcttctc
     1021 cctcggcgcc gtcctctacg agatgctctc gggcaagcgg gccttccgga aggagtcggc
     1081 cgcggagacg acggccgcga tcctcctcct ggagccgccc gagccggcgg cgtcgcttcc
     1141 cggcgtctcg cccgcgctcg cccgggtcgt cgaacgctgc ctcgagaagg agccggagaa
     1201 gaggttcgcg tcggcgcggg agctgatcgc ggcgctggcc gaggcgcaga cggggacggc
     1261 cacgggcgtc gtgacgggcg ccgcggacct cacggggacg cggaagggcg ctcgcctgcc
     1321 tctcgtcgcg ggcctcgtcg tctccgtcgt cgcggcgctc tcggcggcct ggctcgcgcg
     1381 gcggcccgga ccggacacgg acgagagcgc ctcatcacgg cggatcgcgg tccttccgtt
     1441 cgagaacgtg ggccccgccg aggacgacta cctcgccgac gggatcgccg acgaggtccg
     1501 cgggaagctg acctcggtcc cggggctcga ggtgatcgcc cggggcagct cgacgccgta
     1561 tcggcggacc ccgaagacgc ccgggacggt cgcgcgggag ctggacgtcc ggtacctcct
     1621 gacggcgacg gtgcgatggg agagggcgaa ggagcggagc cgcgtcctcg tcagccccga
     1681 gctcgtcgag gtgacggggc ggggggagcc ggtgtcccgc tggcagcagc cgttcgaggc
     1741 ggagctgacc gacgtcttcg cggtccaggc ggagatcgcg gcgcaggtcg tccgcgagct
     1801 cgggatcgcg ctcggcgccg ggacggagaa gcggcttctc gagcggccga cgcgggacgt
     1861 ggcggcgtac gacgcctttc tgagggggga ggcggcctcg gcgagcttcg gggtgaccga
     1921 cccggcaagc ctccgcaggg ccgtcgccct ctacgaggag gccgtcgcgc tcgaccccgc
     1981 cttcgcccag gcctgggcgc ggctcgcccg cgcgtgcgcg cacctccaca cgaacagcgt
     2041 cccgacgccc gcgatgctcg accgcgcgcg ctccgcggcg gagcgcgcgc tggccctcgc
     2101 cccccgcagc ccggaggccc acctcgcgca ggccgcctac gtcggcctcg cgttcgcgga
     2161 cccgaacctc gagctcgacc tcctggagaa ggcccggcgg ctcgcgccgg acgaccccga
     2221 ggtcctgacg gccgccgcgc tcgtcgagca gtcgctcggc cggtgggacg acacgctcgc
     2281 gcacctgaag cgggccgagc ggctcgaccc gcggtcggtc ctgacgaagc gccgcctcgc
     2341 gctcgcctat ctcctcctgc ggcgccagag cgaggctctc gagacgatcg agctcggccg
     2401 gaagatcgcc ccctccagcc tcgtcctcgt cgagctgaag gtcgtcgcgc tcctcgcgct
     2461 cggggacgcg cccggcgccc gtgcggtgct cgacaccgtg ccttcggagg tctcgcccga
     2521 ccggctcctc gcccatctcg ccggtttcca ggacctcggc tggctcttcg acgaacggcg
     2581 gctccggctt ctgctcgacc tcccgccggc cgcgttcgac ggggaccgcg ggacgtgggg
     2641 cctgcgcctt tcgcgcgcct ccgcgcgggc cggaaacgac gcccgtgccc gcgccctcgc
     2701 cggggaggcc cggagggcgt tcgaggagca gctccgcgcc tcgcccgacg acccggagag
     2761 gctcgcctcc ctcggtctcg ccctcgcgta cctcgggcgg gccgcggagg ccgcgcgggc
     2821 gggagagcgt gccgttgccc tcgtccccgt ggagaaggac gcctaccgcg gtccgctcct
     2881 cctccaccag ctcgcgcgca tccgcgccgc gacgggacgg aacgaggagg cgatcgacgc
     2941 cctcgagagg cttcttgcgg tcccgtacac gctcaccccc gcgtggcttc gcgtcgaccc
     3001 gacgttcgac gggctccgcg cgaatccgag gttccggaag ctcgcggcgg ccgggtagga
     3061 agagcccctg cgccggcccg gaccgtcgac ctagcccggg gccgccagac ccgcggcctc
     3121 gagcagcgcc gagatgtcgg cgggaatcgg cgcgatcgcg tcgacgcgcg cacgggtgac
     3181 cgggtggtcg aacgcgagcc ggcgggcgtg aagcgcctgg cgcgggaagt cgcggcaggc
     3241 cgcctgcagc gaggggtcgg cgatcccttt ccagcgcggc gagccgtagg tcgcgtcgcc
     3301 gacgatcgga tggtggatcg ccttcaggtg gacgcggatc tggtgcgtcc ggccggtgac
     3361 gagcgtgcag aggaggagcg agagccccga ccgctcgccg gtcgactcgg cgagacgctc
     3421 gtagagcgtc gtcgagtcgc gcccctcggt cttcgaggtc gacatccgct tccggtcgtg
     3481 cgggtcgcgg aggatcttca ggtcgacctt tcccttcgcg acggccgtcc gaccgtagac
     3541 gacggcgagg tactccttca cgagagtccg cgccttcatc gccttcgcca tcccggcgtg
     3601 cgcggcctcg gtcttcgcga cgacgaggac gcccgaggtg tccttgtcga ggcggtggac
     3661 gaggccgggg cggtcggcct cgccggccca gtccttcgag cggaacagga gcgcattcac
     3721 gagggtgccg tcgcggtggc cgaccgcggg gtggacgacg aggccgggcg gcttgtcgag
     3781 gacgaggagg tggtcgtcct cgaagaggat cgagaggggg atctcctgcg ggatcagctc
     3841 cggcttcggc ggcgggggag gcgg
//
LOCUS       DAOTEZ010000418         3858 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_17517_length_3858_cov_3.940836, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000418 DAOTEZ010000000
VERSION     DAOTEZ010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17517_length_3858_cov_3.940836"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="P5164_17030"
     CDS             <1..345
                     /locus_tag="P5164_17030"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HRY45639.1"
                     /translation="DRAHFIRAVCLAMRRVLVDYARKRAQAVQAEVSLAEGEVEIPGE
                     SGASVVSAAALSQALDHLAAEDPKASEVATLRLFAELTLEEIAEVTGGTVSTVHRKWV
                     YSKAFLRKELSS"
     gene            558..3011
                     /locus_tag="P5164_17035"
     CDS             558..3011
                     /locus_tag="P5164_17035"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRY45640.1"
                     /translation="MGKAFGPYTVQRLLGGGGFSRVFLATRSFEGQERTYAIKVPLWG
                     SFAEEVARYRLEIAVLSQLRHPNIAGLVDVVPGPDDRPLLVMDYVDGARPITLAAVET
                     SIPIRQRIELFLKVLSALSAAHERGVIHCDLSAGNVVLRADGEPVIIDFGIAKVSWER
                     SGTVTVGPRPLTYEYASPEQYRGEPLSVRTDIYSAGVLLYELLAGALPYEVDRKEPEA
                     CRRAVCDVPAERASQRALRSSGLASGQTRSAAKELRGDLDAILGTALEKDPARRYESA
                     NSFAEDLRAHLELRPVVAKAPGGFERSWRWARRHPAISATAALAVILSVGFGTFHVVR
                     LEREQARARKIAAAFRDVLRGFDPAAVAKNPVTVRDLLDQAAAKAEDQLKDEPLVRGA
                     VLETLGTTYSSLGDLDRAEKLLREAVKLRKGGSLAERAESLIALGATLQRRGQLKEAE
                     PVLREALTLRIKDRGEFDKAVAECMTTLAIVLKDQGNTREAISLIERSLAIEEKVAPG
                     SDAVLESRNELANLLIESTSDYQKAIALHAQNLVDLRKYYGESHPYVALALNNIARAE
                     TDAGRYADADRHFREALALQRKIYGGPHPETALTLHNYGFLRLEEGRLEEAEALTRES
                     LEMRRSVLPKGHSDLAVSLNNLGLIVKRRGHLDEAEACYRESLEITRAADGEDSPEIP
                     VTLMNLGGIYRDKGEFAKAEPLLEDALARDRKFFLSPNVSLVRDMTSLAQTKTGLKKY
                     PEAEALLKEATAALGKTGVPPDHRAYADVQEVYAAVLLKTGRVVEARSVIETALQTFA
                     AKPAVRAGFEKLQASLPSQ"
     gene            3119..3622
                     /locus_tag="P5164_17040"
     CDS             3119..3622
                     /locus_tag="P5164_17040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45641.1"
                     /translation="MELTTAAGQTGSRPTRLLTATCEDAWFRCPSCRTDLLRLPWVLD
                     LPGTTETVTIDVWPAGVRAPYELPVVPRLGVRQIAAILGRTIRSTYALTQDFDDAGLR
                     LPGRDLFVRTVPFFEWLHSHPRLRSRAETLADRDQRREANGGVLRPGPRSNRPQLTGS
                     RGGALDH"
BASE COUNT          651 a         1359 c         1281 g          567 t
ORIGIN      
        1 gaccgcgctc acttcatccg ggcggtttgt ctcgcgatgc ggcgagtcct tgtcgactac
       61 gccaggaagc gggcccaggc ggtgcaagcg gaggtctccc tcgccgaggg cgaggtcgag
      121 atccccgggg agtccggagc gtccgtcgtc tctgccgccg ctctgagcca ggcgctcgac
      181 cacctcgcgg cggaggatcc aaaggcctcg gaagtcgcga cgcttcgcct gttcgcggag
      241 ctcacgctcg aggaaatcgc cgaagtgact gggggcacgg tttcgaccgt ccacaggaag
      301 tgggtctact ccaaggcatt ccttcggaaa gagctctcct cgtgaccgac gctcgcctcg
      361 tgagaaagca agagctcctg gcttcgctgg ccctcgcgtc gaccgaagac cgactccgac
      421 tccttgccga gtcctgcgga gacgatccga gcctccggac cgaagtagag tctcttctcc
      481 cctacctcgg agccccgacg ccatccccgg caggagacct cgatgctgcc ctcgaggaga
      541 aactcgccga gaggttcgtg ggaaaggcct tcgggcccta caccgtccag cggctcctcg
      601 gcggaggcgg gttctcccgc gtgttcctgg cgacccggtc gttcgagggg caggagcgga
      661 cgtacgcgat aaaggtgcct ctctggggct ccttcgccga ggaggtagcg cgctaccgcc
      721 tcgagatcgc agtcctgagc cagctccgcc acccgaacat cgcgggcctc gtcgacgtgg
      781 tccctggccc ggacgaccgg ccgctcctcg tcatggacta cgtggatggg gcgcggccga
      841 tcacgctcgc cgccgtcgag accagcattc cgatccgaca aaggatcgag ctcttcctga
      901 aggtcctctc ggcgctcagc gccgcgcacg agcgcggcgt catccactgc gacctctcgg
      961 ctgggaacgt cgtgcttagg gcggacggcg agccggtgat catcgacttc ggcatcgcga
     1021 aggtttcgtg ggagcgctcc ggcaccgtca cggtcggtcc caggccgctc acgtacgagt
     1081 acgcgagccc cgagcagtac cgcggagagc ccctctcagt ccgcaccgac atctactcag
     1141 caggggttct cctctacgag cttctcgctg gtgccctgcc ctacgaggtc gatcggaagg
     1201 agcccgaggc gtgccggcgg gcggtctgcg acgtgccggc cgaacgggca agccagcggg
     1261 cgctccggtc atcgggactc gcctccgggc agaccaggtc cgccgcgaaa gaactccgcg
     1321 gggacctcga cgcgattctg ggcacggcgc tcgagaaaga ccccgcccgt cgatacgagt
     1381 cggcgaactc ctttgcagag gacctccgtg cgcacctgga gctccgcccc gtcgtggcga
     1441 aggcgccggg cggcttcgag cggtcctggc gctgggcgag gcgccacccg gcgatctcgg
     1501 ccacggcggc tctcgcggtc atcctctccg tcggcttcgg caccttccac gtcgtccgcc
     1561 tcgaacggga gcaggcgcgc gcccggaaga tcgcagcggc gtttcgggac gtcctgcggg
     1621 gattcgaccc ggcggccgtc gcgaagaacc cggtgaccgt gcgagacctg ctggaccagg
     1681 ccgccgcgaa ggcggaagac cagctgaagg acgaaccgct cgtccgcggg gcggtcctcg
     1741 agacgctcgg gacgacgtac tcgtcgctcg gagacctgga tcgagctgag aagctcctcc
     1801 gggaagcggt caaacttcgg aagggcgggt cgctcgccga gcgggcggag agcctcatcg
     1861 cactgggtgc gaccctccag cggcgtgggc agctcaagga agcggagccg gttctccggg
     1921 aggctctgac gctgcggatc aaggaccgag gggagttcga caaggcggtc gccgagtgca
     1981 tgacgacgct cgccatcgtc ctgaaggacc aggggaacac gcgggaggcg atctcgctca
     2041 tcgagcgctc gctcgcgatc gaagagaaag tcgcccctgg gagcgacgcg gtgctcgagt
     2101 cgcgcaacga gctcgcgaac ctcctcatcg aatcgacgag cgactaccag aaggccatcg
     2161 ctctccacgc gcagaacctc gtcgacctgc ggaagtacta cggagagagc cacccgtacg
     2221 tcgctctggc tctgaacaac atcgcccggg ccgagacgga cgcgggccgc tacgcggacg
     2281 cagaccggca cttccgggaa gcgctcgcgc tccagcggaa gatctacggc ggaccgcacc
     2341 ccgaaacggc gctcacgctg cacaactacg gcttcctccg tctggaggag ggacgactcg
     2401 aagaggccga ggcgctgaca cgcgaatcgc tcgagatgcg ccggagcgtc ctgccgaaag
     2461 ggcactcgga cctcgcggtg agcctcaaca acctcggcct catcgtgaag cgccgcgggc
     2521 acctcgacga ggccgaggcg tgctaccgcg agtcgctcga gatcacccgg gcggcggacg
     2581 gcgaggacag cccggagatc cccgtcacgc tcatgaacct cggcgggatc taccgcgaca
     2641 agggcgagtt cgcgaaggcg gaacctctcc tcgaagacgc cctcgcccgg gacaggaagt
     2701 tcttcctctc cccgaacgtg tccctcgtgc gcgacatgac ctcactcgcc cagacgaaga
     2761 cagggctcaa gaagtacccc gaggcggagg cgctcctgaa ggaggcgacc gccgccctcg
     2821 ggaagaccgg cgtcccgcca gaccaccgcg cgtacgcgga cgtgcaggag gtctacgcgg
     2881 cggtgctcct caagaccggt cgcgtcgtcg aggcccggtc agtcatcgag acggcgctcc
     2941 agacattcgc ggccaagccc gccgtgcgcg ccgggttcga gaagctccag gcctccctcc
     3001 cgagccaata acgagtattt cgtccgtctc gcgagaaaaa cgactccatt tccgcgctct
     3061 acggggtagc gagctacgga cgtcccgaga catccgcaga cgcgagaagg agagcgccat
     3121 ggagctgacc accgcggccg gacagaccgg ctcccgcccc acgcgtctgc tgaccgccac
     3181 ctgtgaagac gcatggttcc gctgcccgtc gtgccgaacg gacctcctgc gcctgccgtg
     3241 ggtcctggac ctgcccggga cgacggagac cgtcacgatc gacgtctggc cggccggcgt
     3301 ccgcgccccc tacgagctgc cggtcgtgcc ccgcctcggg gtccggcaga tcgcggcgat
     3361 cctcggccgg acgatccgtt ccacgtacgc cctcacccag gacttcgacg acgccggact
     3421 ccgcctcccg ggccgggacc tcttcgtccg gacggtcccc ttcttcgagt ggcttcacag
     3481 ccacccgcgg ctgcggtccc gggcggagac gctggccgat cgagaccagc ggcgagaggc
     3541 caacggaggg gtcctccgtc ccggtccgcg ttcgaatagg ccccagctca ccgggtctcg
     3601 gggaggcgca ctcgatcact gaggtccctg gtatagcggg actgtagaaa acgactctga
     3661 gcgcccactt cgtagcggct gctgtactgc tgacagaccg agacggagga cgagggcgct
     3721 ccgagtttcg cctcacgggc ggcatggcgc cgtccgtgag gctctccgga tttccctact
     3781 tcccgaccga cgcgtcgttc acacgattga ggacattggc cacctcgccg aggacccgct
     3841 cgtcccctcg agcgtagc
//
LOCUS       DAOTEZ010000419         3854 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_17539_length_3854_cov_5.937352, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000419 DAOTEZ010000000
VERSION     DAOTEZ010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17539_length_3854_cov_5.937352"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..1198)
                     /locus_tag="P5164_17045"
     CDS             complement(29..1198)
                     /locus_tag="P5164_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRY45642.1"
                     /translation="MAEDTHAPTPLAAGAPAPPFTLPGRSGSVVSLADFAGKGHVLVY
                     FYPKDDTPGCTKEACSLRDGFSDLRAAGVTVLGISPDGAASHAAFAEKYSLPFELLTD
                     ADHSVHEAYGVWGKTPWGIGTIRRSFLVGPDGAIVRVFDDVDVERHAGQVLAALKPTP
                     VAEAFAAVEAAVEAAGAAVSTAVSESRKAVKGAMKSPEVKDAVAAAKKAVADVKKQPA
                     VKKAVATAEKAVSDVKGAVAAAKKKPAVKKAVAEAKKAVAGAKRAVADVKRKPAVKRA
                     VAKAKKAVADAKKRPVVKKAVASAKKAVARAKTAAAKKPKGALAKLLALVTPAKAAPK
                     GASRKKASPRKPARKPAPKKAAPVRKATPRPAVKKATPARKPAPRKAPRRTAARR"
     gene            1255..2001
                     /locus_tag="P5164_17050"
     CDS             1255..2001
                     /locus_tag="P5164_17050"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRY45643.1"
                     /translation="MTVPSALSVSAADPAGGLLGADLLAFGALGVAGSGVVTAVTAGG
                     LRSPGPAQEVPVALLRASLDAAFAASRFAAVKVGVVPSAPAARAIARALAPLRAPRVL
                     HPGFAPASEVRLVRATALDAVVRDLFPGAALVVLGQAEASALAGFPIREENDVKAAAR
                     RVQALGPAAVLVTGGSAAGDRVVDGLLDGRTWHRFDAERLPPPSDLTAGDRLSAAVTA
                     LLATGETLPDAVARARSWGSGLPSTLYGNP"
     gene            complement(1986..2948)
                     /locus_tag="P5164_17055"
     CDS             complement(1986..2948)
                     /locus_tag="P5164_17055"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY45644.1"
                     /translation="MPTPFPVGPINTHLVLGEPITLVDTGPLTEDAWAALRAGLSRHG
                     LGVGDVERVLLTHGHQDHFGLAARIEREGRAQLFGGRLDREHFRGRRNRRLLLDALAR
                     AGFGALERLTMVAMTAAVDHFADPLPAWDELSGGEVLPGDGWSVVVRSVPGHTPGSLT
                     FEVPEAGVLFTGDTVLRDITPNAIVDEDPERPGEAFRSVSRYFASLDSIEAGESALLT
                     GHGGRIVDFAAHRARLEGRYVFRVGQIERALSGRPKSVSELCRRIFPTVRPMDLFLAY
                     SEVLGFLMYLEDLGRAERIPGRAVDRWTRSEKGAELPSSLYGFP"
     gene            complement(3067..>3854)
                     /locus_tag="P5164_17060"
     CDS             complement(3067..>3854)
                     /locus_tag="P5164_17060"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRY45645.1"
                     /translation="EMTAHTYVLESMVYMTAGLVDKKVDYSLEGACCKVWGTELLWKT
                     INESLQIAGGNGFMNEYPYGRGLRDSRINMIFEGTNEILRLFIALTGMKEAGDVLKDF
                     QGALSHPVAQYGVLVDHARMWMRGTVTSYKLDKVHPSLAPEADQVGKYAAVLRNACET
                     MVYRNRKNVVNRQYLLHRIADVVMDLYAQIATLSRVTAAIEKKGEEKARPEIEIVRWF
                     SFQARHRMVANLKALDKHKDAEATAISNVVYEAGGYPFDLWGW"
BASE COUNT          500 a         1401 c         1344 g          609 t
ORIGIN      
        1 ggaacgcctc ctcgggggcc ttctccatct agcggcgggc ggccgtcctc cggggcgcct
       61 ttctcggcgc gggcttccgg gcgggtgtcg ccttcttcac ggccggcctg ggggtcgcct
      121 tccggacggg cgcggccttc ttcggcgccg gcttgcgggc gggcttgcga ggagaagcct
      181 tcttgcggga agcccccttc ggggccgcct tcgccggcgt cacgagcgcc aggagcttcg
      241 cgagcgcgcc cttcggcttc ttcgccgcgg ccgtcttcgc gcgggcgacc gccttcttcg
      301 cggaggccac ggccttcttc accacggggc gcttcttcgc gtccgcaacg gccttcttcg
      361 ccttcgcgac ggcccgcttc acggcgggct tcctcttcac gtcggcgacg gctctcttcg
      421 ccccggcgac ggccttcttc gcctccgcga ccgccttctt cacggccggc ttcttcttcg
      481 ccgcggcgac ggcgcccttc acgtcggaga cggccttctc cgccgtcgcg acggccttct
      541 tcacggccgg ctgcttcttc acgtccgcga cggctttctt cgcggccgcg acggcgtcct
      601 tcacctcggg cgacttcatc gcgcccttca cggccttccg agactccgag acggccgtcg
      661 agacggcggc gccggcggcc tcgaccgcgg cctcgaccgc ggcgaaggcc tcggcgaccg
      721 gagtcggctt caacgccgcg aggacctgcc cggcatggcg ctcgacgtcg acgtcgtcga
      781 agacgcggac gatcgcgccg tccggaccga cgaggaacga gcggcggatc gtgccgatcc
      841 cccagggggt cttcccccag acgccgtacg cctcgtgcac cgagtgatcg gcgtccgtca
      901 ggagctcgaa cgggagggaa tacttctccg cgaacgcggc gtgcgacgcg gcgccgtccg
      961 gagagatccc gagaaccgtc acgccggcgg cgcgcaggtc cgagaagccg tcgcggaggc
     1021 tgcaggcctc cttcgtgcat cccggcgtgt cgtccttggg gtagaagtag acgaggacgt
     1081 gtcctttccc ggcgaaatcc gccagggaga cgacgcttcc ggagcgcccg ggaagcgtga
     1141 acggaggggc cggggcgccg gccgcgagcg gagtcggggc gtgcgtatct tcagccactt
     1201 tgtgtcttcc tccggacgtc gaggatagca ggaggagcgc ctaaactgac ggggatgacc
     1261 gtgccgtccg ccctctccgt ctcggccgcc gaccctgccg gaggactcct cggggccgac
     1321 ctcctcgcct tcggggccct gggcgtcgcc gggtcgggcg tcgtcaccgc cgtgaccgca
     1381 ggtgggctgc gctccccggg ccctgcccag gaggtgcccg tggccctcct ccgggccagt
     1441 ctcgacgccg ctttcgcggc gagccggttc gcggccgtga aggtcggggt cgtcccttcc
     1501 gccccggccg ctcgggccat tgcccgcgcg ctcgctccgc ttcgtgcccc gagggtcctc
     1561 caccccggct tcgccccggc ctccgaggtc cggctcgtcc gggcgaccgc cctcgacgcg
     1621 gtcgtaaggg acctctttcc gggcgccgcg ctcgtcgtcc tcggacaggc agaggcctcc
     1681 gcgctcgcgg ggttcccgat ccgcgaggag aacgacgtga aggctgcggc ccgccgggtc
     1741 caggccctgg gaccggcggc cgttctcgtg accggcggca gcgcggccgg cgaccgggtc
     1801 gtcgacggtc tcctcgacgg acggacgtgg caccgcttcg atgccgaacg cctcccgcct
     1861 ccatccgacc ttacggccgg cgaccgcctc agcgccgcgg tgacggcgct cctcgccacc
     1921 ggcgagaccc tccccgacgc cgttgcccgc gcccgcagct gggggtcagg tctaccatct
     1981 acactctacg gaaatccgta gagcgaggag ggtagctctg cccccttctc tgaacgtgtc
     2041 cagcggtcga cggcgcggcc gggtatccgc tccgcgcggc cgaggtcctc gaggtacatc
     2101 aggaagccga gcacctccga gtaggcgagg aagaggtcca tcggccggac ggtcggaaag
     2161 atgcggcggc agagctccga cacggacttc ggccttcccg agagcgctct ctcgatctgg
     2221 ccgacacgaa agacgtagcg cccctccagc ctcgcgcggt gggcggcgaa gtcgacgatc
     2281 cggccgccgt ggcccgtgag gagggccgac tcccccgcct cgatcgagtc gagcgaggcg
     2341 aagtagcgcg acacgctccg gaacgcctct cccggccgct ccgggtcttc gtcgacgatg
     2401 gcgttcggcg tgatgtcgcg gaggacggtg tcgcccgtga agaggacgcc ggcttccggg
     2461 acctcgaacg tcaggctccc gggcgtgtgg ccgggaacgg accggacgac gacggaccac
     2521 ccgtcgcccg ggaggacctc tccacccgaa agctcgtccc aggcgggaag cgggtcggcg
     2581 aaatggtcga cggcggccgt catcgccacc atcgtaaggc gctccagcgc gccgaagccg
     2641 gctcgggcga gcgcatccag gaggagccga cgattccggc gacctcggaa gtgctcccgg
     2701 tcgagccgcc cgccgaagag ctgcgccctg ccctcccgct cgatgcgcgc cgcgaggccg
     2761 aagtggtcct ggtgcccgtg cgtcaggagg acgcgctcga cgtcccccac gcccagaccg
     2821 tgccgggaga ggccggcgcg gagcgcagcc caggcgtcct cggtgagcgg tcccgtgtcg
     2881 acgagagtga tcggctcgcc gaggacgagg tgagtgttga tggggccgac gggaaacggt
     2941 gtcggcaccg ggatcgtgac gacacgaggc gcacgcgcgc cgccgtgaag ggcaggatgg
     3001 tccgccgatc ggcgggcggg agggacccgg cggggggccg gacgcgtccg gccccccgcc
     3061 cgcggctcac cagccccaga ggtcgaaggg gtacccaccc gcctcgtaga cgacgttgct
     3121 gatcgccgtc gcctcggcgt ccttgtgctt gtcgagggcc ttcaggttcg cgaccatccg
     3181 gtgccgggcc tggaacgaga accagcggac gatctcgatc tccggcctcg ccttctcttc
     3241 gcccttcttc tcgatcgcag ccgtcacgcg cgagagcgtg gcgatctggg cgtagaggtc
     3301 catgacgacg tcggcgatcc ggtggaggag gtactgccgg ttcacgacgt tcttccggtt
     3361 ccggtagacc atcgtctcgc aggcgttccg gaggacggcc gcgtactttc cgacctggtc
     3421 cgcctccgga gcgagggacg gatggacctt gtcgagcttg tagctcgtca cggtgccgcg
     3481 catccacatc ctcgcgtggt cgacgaggac gccgtactgc gcgacggggt ggctgagcgc
     3541 cccctggaag tccttcagca cgtcgcccgc ctccttcata ccggtcaggg cgatgaagag
     3601 gcgaaggatc tcgttcgtcc cctcgaagat catgttgatg cggctgtcgc gaaggccccg
     3661 gccgtacggg tactcgttca tgaagccgtt gccgccggcg atctggagcg actcgttgat
     3721 cgtcttccag aggagctccg tcccccagac cttgcagcac gccccctcga gcgaatagtc
     3781 gaccttcttg tccacgagcc cggcggtcat gtagaccatc gactcgagga cgtaggtgtg
     3841 cgccgtcatc tcgg
//
LOCUS       DAOTEZ010000420         3847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_17579_length_3847_cov_6.020833, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000420 DAOTEZ010000000
VERSION     DAOTEZ010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17579_length_3847_cov_6.020833"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="P5164_17065"
     CDS             complement(<1..323)
                     /locus_tag="P5164_17065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45646.1"
                     /translation="MRRVTEVPEVTEPPPALPAPSPRVERAIVLALVVLSAVLALRHL
                     SGDAATADEPVHVAAAVEIAREGTGRWNPEHPPLAKALAGLALTGLPLRPAEDPLRTP
                     AGPARL"
     gene            342..995
                     /locus_tag="P5164_17070"
     CDS             342..995
                     /locus_tag="P5164_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HRY45647.1"
                     /translation="MSTVRMHSLTLPGPAGDLEALLRVPVRPRGAAVVAHPHPLFGGT
                     MHTKVVHRAAKVFSDRLHLSALRFNFRGVGASEGSYDEGRGETDDLVAAVHWARQRQP
                     SGPLALAGFSFGSLCAARAAVSVAPHVLLLVGVPLDRWDEEDARALEGRNVVWVQGER
                     DSFGAGALALETAARHGFRAASVPGADHFFTGRLEGFEEAALRLLGEAGFGPPVGPA"
     gene            992..1570
                     /gene="amrA"
                     /locus_tag="P5164_17075"
     CDS             992..1570
                     /gene="amrA"
                     /locus_tag="P5164_17075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HRY45648.1"
                     /translation="MTERGETMLTDAERDDLLRRARHAAARTLGLPEGKRELPLPAGR
                     LAAPGASFVTWKRDGRLRGCIGSVEPWRPLAEDVERNAVAALVRDPRFAPAQARDLRW
                     LSVEVSVLGPLEEVSEPLGQLEIGVHGVVVKKGSRSGLLLPQVAPEWGWDVPTLLAQV
                     CLKAGLPETAWREGSPPATVYRFSAEVFGESA"
     gene            complement(1531..2316)
                     /locus_tag="P5164_17080"
     CDS             complement(1531..2316)
                     /locus_tag="P5164_17080"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRY45649.1"
                     /translation="MSVRIDSPSNPRIAAAARAVREGEALLLEGARTLEDALDAGVVP
                     DVVFAAPDLVPVDASAAERAREAGAAVVEVSARVLARLSELGSTRALVSLAPVPSAAL
                     GSLSGVPLVLLLDGLQDPANVGAILRAAEAFGAGGVILTPGTASPYAPKAFRASAGSA
                     LRVPVVRNVLSTDAVRWVRSTGALLAGADAHGGEGPGALEGAAPLVLAIGSEGHGLSA
                     PLVAALDRRVTIPVSPRVESLNAAVATGILLYALSPKTSAEKR"
     gene            complement(2313..2894)
                     /locus_tag="P5164_17085"
     CDS             complement(2313..2894)
                     /locus_tag="P5164_17085"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HRY45650.1"
                     /translation="MPPPLPIPESYWVVPGLLLAGEYPYSLVEDDGRERLRAFLDAGI
                     RLFVDLTEPRESGRLGALEPYAAPLLEEAAARGIAVRVVRHAVKDMSVPRDGSLERIV
                     EELEGAVARGEPAYVHCWGGIGRTGTAVAAFLSRRLGLTGDEALDLLDALWQEVPKSA
                     IFPESPQTDEQRRAVRAFAGPRGSQATEGRGPE"
     gene            3071..3493
                     /locus_tag="P5164_17090"
     CDS             3071..3493
                     /locus_tag="P5164_17090"
                     /inference="COORDINATES: protein motif:HMM:NF024246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRY45651.1"
                     /translation="MKQLRIDVGRCDYGRHCEHECEHACAEKVFKLKDPAMAALHIRE
                     LPDGKGVAALCDQCGDCVVVCPADALRRNKPGVILLDKKLCVGCYMCVGFCEKDVFER
                     NPEWLEPYKCISCGICVKVCPRAALEIVDVPEPAPRII"
     gene            3510..>3847
                     /locus_tag="P5164_17095"
     CDS             3510..>3847
                     /locus_tag="P5164_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRY45652.1"
                     /translation="MSQVVFDPAKVMDVDYTKRTEYHAELEAMRVSRRLLKEVAFTPS
                     LPERGYNGRTLHVDVGTLAVTEKPVTPQMKDLFVGGRGFGLWHLWNAVTPRTRWNDPE
                     NEIVISPGPV"
BASE COUNT          512 a         1376 c         1496 g          463 t
ORIGIN      
        1 agccgcgcgg ggccggcggg ggtgcggagc gggtcctcgg ccgggcggag cggaaggccg
       61 gtcagcgcga ggccggcgag cgccttggcg agcggcgggt gctccgggtt ccagcgcccg
      121 gtcccctcgc gcgcgatctc gacggcggcc gcgacgtgga ccggctcgtc ggccgtggcg
      181 gcgtctcccg ataggtgccg gagcgccagg acggcggaaa ggacgacgag cgcgaggacg
      241 atcgcgcgtt ccacgcgggg cgacggcgcg ggcaacgccg gcgggggctc ggtcacctcg
      301 ggcacctcgg tcactctgcg catgctaact tcgggaacgc cgtgagcacc gtccgaatgc
      361 actccctcac gctgcccggg cccgccgggg acctggaggc cctcctgcgc gttccggtcc
      421 ggccgcgcgg ggccgcggtc gtggcgcacc cgcatcctct atttggcgga acgatgcaca
      481 cgaaggtcgt ccatcgggcc gcgaaagtct tctcggaccg gctccacctc tcggccctcc
      541 gcttcaactt ccggggcgtc ggcgcttcgg aagggagcta cgacgagggg cgcggcgaga
      601 ccgacgacct cgtcgccgcc gtccactggg cgcggcagcg ccagccgtcc gggccgctcg
      661 cgctcgccgg cttctcgttc gggtcgctct gcgcggcgcg ggcggccgtc tcggtcgcgc
      721 cgcacgtcct cctcctcgtc ggggtgccgc tcgaccggtg ggacgaggag gacgcccgcg
      781 cgctcgaagg ccggaacgtc gtctgggtcc agggcgagag ggactcgttc ggcgcgggcg
      841 cgctcgccct cgagacggcg gcgcgccacg gcttccgggc ggcgtccgtc ccgggagcgg
      901 accatttctt caccggccgc ctggaggggt tcgaagaggc ggcgctccgc ctcctcggcg
      961 aggcgggctt tggcccgccg gtcgggccgg cgtgacggaa aggggcgaga cgatgctcac
     1021 cgacgccgaa agggacgacc tcctccggcg ggcgcgccac gccgccgcgc ggacactcgg
     1081 cctcccggag ggaaagcgcg agctgccgct cccggccggg cgcctcgccg ccccgggcgc
     1141 gtcgttcgtc acctggaagc gggacggccg gctccgcggc tgcatcggga gcgtcgagcc
     1201 gtggcggccg ctcgccgagg acgtcgagag gaacgccgtc gcggcgctcg tgcgcgaccc
     1261 gcgattcgcg ccggcccagg cgcgggacct ccgctggctc tccgtcgagg tctccgtcct
     1321 cggtccgctc gaggaggtct cggagccgct cgggcagctc gagatcgggg tccacggcgt
     1381 ggtcgtgaag aagggctcgc gctccggcct gctcctgcct caggtcgccc ccgagtgggg
     1441 ctgggacgtc cccaccctcc tcgcgcaggt ctgcctgaag gcgggcctcc ccgagacggc
     1501 ctggcgggag gggagccctc ccgcgacggt ctaccgcttc tccgccgagg tcttcgggga
     1561 gagcgcgtag aggaggatcc cggtcgcgac cgccgcgttg agcgactcga cgcgcggcga
     1621 gacgggaatc gtcacacgac gatcgagcgc ggcgacgagc ggcgccgaga gcccgtgccc
     1681 ctcggagccg atcgcgagga cgagcggagc ggcgccctcg agagcgccgg gcccctcgcc
     1741 gccgtgcgcg tcggcgcccg cgaggagagc gccggtggaa cggacccacc gcaccgcgtc
     1801 ggtcgacagg acgtttcgga cgaccgggac gcgaagggcg ctcccggcgg aggcgcggaa
     1861 cgccttcggg gcgtacgggg aggcggtccc cggggtcagg atcactccgc ccgccccgaa
     1921 ggcctcggcc gcgcgaagga tcgcgccgac gttcgccggg tcctggaggc cgtcgaggag
     1981 gagcacgagc ggcacgcccg agagggagcc gagggccgcg gacgggaccg gcgcgagcga
     2041 gacgagggcc cgcgtcgagc cgagctccga gaggcgcgcg aggacgcgtg cggagacctc
     2101 gacgaccgcg gccccggcct cgcgggcgcg ctcggccgcg gacgcgtcga cgggcacgag
     2161 gtcgggggcg gcgaagacga cgtcggggac gacgccggcg tcgagggcgt cctcgagggt
     2221 cctcgccccc tcgaggagga gcgcctctcc ctctcgcacg gcgcgggcgg cggccgcgat
     2281 gcgagggttc gagggcgagt cgatccgcac gctcattccg gcccccgtcc ttcggtcgcc
     2341 tgggagccgc gcggcccggc gaacgcgcgg acggcccggc gctgctcgtc cgtctgcggc
     2401 gactcgggga agatcgcgct cttcgggacc tcctgccaga gcgcgtcgag gaggtcgagg
     2461 gcctcgtcgc cggtcagccc gagtcgccgc gagaggaacg cggcgacggc cgtgccggtc
     2521 cggccgatgc ctccccagca gtggacgtag gccggctccc cgcgcgcgac cgcaccctcg
     2581 agctcctcga cgatccgctc gaggctcccg tcgcgcggca cgctcatgtc cttcacggcg
     2641 tggcggacga cgcggaccgc gatgccgcgc gcggcggcct cctcgaggag gggggcggca
     2701 taaggctcga gggcgccgag gcgtccgctc tcgcgcggct ccgtgaggtc gacgaacagg
     2761 cggatgccgg cgtccaggaa cgcccggagg cgctcccgcc cgtcgtcctc cacgagcgag
     2821 taggggtact cgcccgccag gaggagcccc gggacgaccc agtacgactc gggaatcggc
     2881 aggggagggg gcatccggcg cgagtctagt ccgatccggg gcgcgggagg ccgggcgggg
     2941 gaagcgtcac gcgacccggc gaggacgtgg ctttgaagcc ctccgcccgg atggtaccgt
     3001 tgaaggtcgg ccgacggggc cgggcttcgc ggcccgaggc ccgacgcggc cgcgggacgg
     3061 gagaccgggc atgaagcagc tgcggatcga cgtgggcagg tgcgactacg ggcgccactg
     3121 cgagcacgag tgcgagcacg cgtgcgccga gaaggtcttc aagctgaagg acccggcgat
     3181 ggcggcgctc cacatccgcg agctgcccga cgggaaaggg gtcgccgcgc tctgcgacca
     3241 gtgcggcgac tgcgtcgtcg tctgccccgc ggacgcgctc cggcggaaca agccgggcgt
     3301 catcctgctc gacaagaagc tctgcgtcgg ctgctacatg tgcgtcggct tctgcgagaa
     3361 ggacgtcttc gagcggaacc cggagtggct cgagccgtac aagtgcattt cctgcggcat
     3421 ctgcgtgaag gtctgcccga gggcggccct cgagatcgtc gacgtccccg agcccgcgcc
     3481 gcggatcatc tgaaggaagg gcccgagcca tgtcccaagt cgtcttcgac ccggccaagg
     3541 tgatggacgt cgactacacg aagcggaccg agtaccacgc ggagctcgag gcgatgcgcg
     3601 tgtcgcgccg gctcctgaag gaggtcgcct tcacgccgag cctccccgag aggggctaca
     3661 acggccgcac cctccacgtc gacgtcggga cgctcgcggt gacggagaag cccgtcaccc
     3721 cgcagatgaa ggacctcttc gtcggcgggc gcggcttcgg cctctggcac ctctggaacg
     3781 cggtcacccc gaggacgcgc tggaacgacc ccgagaacga gatcgtcatc agcccggggc
     3841 cggtctg
//
LOCUS       DAOTEZ010000421         3772 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_18114_length_3772_cov_6.927361, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000421 DAOTEZ010000000
VERSION     DAOTEZ010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18114_length_3772_cov_6.927361"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1940)
                     /locus_tag="P5164_17100"
     CDS             complement(<1..1940)
                     /locus_tag="P5164_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HRY45653.1"
                     /translation="MGRDLAAVVGANRRGDGCLVGDGWVVTWAVGHLVGLAEPGRMNP
                     EWQAWRRDRLPMIPSTWPLVVLDRAADQFAAVRRVLSDPDVSEVVCATDAGREGELIF
                     RQLYESARCTKPVRRLWISSLTPEAIRDGLARLKPARDYDRLADAARGRSRADWLVGM
                     NFSRAFTLAHGEPLTVGRVQTPTLALLVERELAIRAFVPEDYREVVATFEGETGRYCG
                     VWFDPAMKEDGGRRLPRDGSAAAAIVARVRDGGARVASVESKTRRTPPPALFDLTELQ
                     RHANRLLGWSAKKTLDVAQRLYEERKAITYPRTDSRHLSKDVAATLPALLGALEPEWG
                     RLYAKGAGERPLGARFVDDAKVSDHHAIVPTTSVPRGLAGDEKALYEIVVRRLLGAWH
                     DDHVAATTTVVTEVSGNGGAEGPAAGALAGRTVDRFRSAGTMVVEEGWKAAEPDAPRR
                     RRNEDDEEGDLPAGLAEGQAKRVVDAASVEKRTRPPKRFTDASLLTAMESAGRTLDEK
                     ELAEAMKENGLGTPATRAEVLENLLARGYAERDGKALVATEKGIRLVGLVPESVKSPA
                     LTGRWEAELARLQKGDGDLASFLRGIEAYVTSVVADAFRAPAVRPASEPADSSGLRKV
                     PRPATEPAGTVPGATETTAVSPP"
     gene            complement(2129..>3772)
                     /locus_tag="P5164_17105"
     CDS             complement(2129..>3772)
                     /locus_tag="P5164_17105"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRY45654.1"
                     /translation="RLVAGRGFTDEELVLGARLPLEGSASGRAVRERRIVSLGEVRIE
                     GREVHPVLDALARRHVASGLCIPLAHGGRPLGTVNVIFPEGRPLRPEEAETFEGVARA
                     VSLAVANAGHRSELEERAFRDALTGLPNRAGFHRRFGTLAETAGGGRAALVLLDLDWF
                     REINDTLGHNFGDELLVQVGARLAERCADGMEVFRLGGDEFAIVVPGAERREDGEAAA
                     RKVLAWLGEPFRVAGMGLELGASAGVAHYPAHARVSHGLLRCADVALHVAKKGSGGVA
                     CYEPDLDEHTPERLSILSDLGRAIREGQLVLHFQPKVALQYDFIEGFEALVRWRHPRL
                     GLLGSSQFVPFAEATDGMVALTSWVVRRALEQLSRWNRQLPKLTMAVNLSARNLVDRS
                     CPEKLEEIVRSVGVDPGLVEFELTESAVMSDPETALKMLGRLHDSGARLAIDDFGTGY
                     SSLAYLTRLPVDVLKIDRSFVAGMAAGGKDLAVVRSTVQLAHSLDLGVVAEGVEDAAT
                     ARSLREMGCDLGQGFLFAPPAPAEGFERLLAGGGFLAGR"
BASE COUNT          495 a         1472 c         1308 g          497 t
ORIGIN      
        1 gggggggaga ccgcagtcgt ctcggtcgcg ccgggaaccg tgccggcggg ctcggtcgcc
       61 ggccggggca cctttcgaag accggaggaa tccgcaggtt ccgaggccgg gcgcacggcc
      121 ggcgcgcgga aggcgtcggc gacgaccgat gtcacgtacg cctcgattcc gcgcagaaag
      181 gaggcgaggt cgccgtcgcc cttctgtagg cgggcgagct cggcctccca gcgccccgtc
      241 agggcagggc tcttcaccga ttccgggacg aggccgacga ggcgaatccc cttctccgtc
      301 gccacgaggg ccttcccgtc tcgctccgcg tagccgcgcg cgagcaggtt ctcgaggacc
      361 tcggcgcgcg tcgccggcgt cccgaggccg ttctccttca tcgcctcggc gagctccttc
      421 tcgtcgagcg tccggccggc ggactccatc gccgtcagga gcgatgcatc ggtgaaccgc
      481 ttcggcgggc gggtccgctt ctcgacgctc gccgcgtcga cgaccctctt cgcctgcccc
      541 tccgcgaggc ccgccgggag gtcgccctcc tcgtcgtcct cgttcctcct ccgccgcggc
      601 gcatcgggct cggccgcctt ccacccttct tcgacgacca tcgtcccggc ggaccggaat
      661 cgatcgacgg ttcgcccggc gagcgccccg gcggccgggc cctccgcgcc cccgttcccc
      721 gacacttccg tcacgacggt cgtcgtcgcg gcgacgtggt cgtcgtgcca cgcgccgagg
      781 aggcggcgga cgacgatctc gtacagggcc ttctcgtctc ccgccaggcc gcgcggaacg
      841 ctcgtcgtcg ggacgatggc gtggtggtcg ctcaccttcg cgtcgtcgac gaagcgtgcg
      901 ccgagcggcc gctcgcccgc ccccttcgcg tagagccgcc cccattccgg ctcgagcgcc
      961 ccgaggagcg ccgggagcgt cgccgcgacg tccttggaaa gatggcggct gtcggtcctc
     1021 gggtaggtga tcgccttccg ctcttcgtag agacgctgcg ccacgtccag cgtcttcttc
     1081 gcgctccagc cgaggaggcg gttcgcgtga cgctgcagct ccgtcaggtc gaagagggcc
     1141 ggcggcggag tgcgccgcgt cttcgactcg accgacgcga cccgcgcccc cccatccctc
     1201 acgcgcgcga cgatcgccgc cgcggcggac ccgtccctcg ggaggcggcg cccgccgtcc
     1261 tccttcatcg ccgggtcgaa ccagacgccg cagtagcgcc ccgtctcccc ctcgaacgtg
     1321 gcgacgacct cccggtagtc ctcggggacg aacgcccgga tcgcgagctc gcgctccacg
     1381 aggagcgcga gggtgggcgt ctggacgcgc cccaccgtca gcggctcgcc gtgcgcgagc
     1441 gtgaaggccc gcgagaagtt catccccacg agccagtcgg cccggctccg tccgcgcgcg
     1501 gcgtcggcga gccggtcgta atcccgtgcc ggcttcagcc tcgcgagccc gtcgcggatc
     1561 gcctcgggcg tgagcgacga gatccacagc cgcctcaccg gcttcgtgca gcgcgccgac
     1621 tcgtagagct ggcggaagat gagctccccc tcccgtccgg cgtccgtggc gcagacgacc
     1681 tccgacacgt ccgggtcgga gaggacccgg cgcaccgccg cgaactgatc cgccgcgcgg
     1741 tcgaggacga cgagcggcca cgtcgagggg atcatcggga gacggtcgcg cctccaggcc
     1801 tgccattcgg ggttcatccg gcccggctcg gcgagcccga cgaggtggcc gacggcccag
     1861 gtcacgaccc acccgtcgcc gacgaggcag ccgtccccgc gccggttcgc cccgacgacg
     1921 gccgcgaggt ccctccccac gctcggcttc tccgcgacga tcgctacggt ccggccgtct
     1981 cgccccggag cccctccgcc cggcggcccc ggctctcgcc ggacgcgccc tctctcgacc
     2041 aacaccggcc tccctcggcc ctctgccagg ccgccggagg acgatagagc atccgaaggg
     2101 cgcgccgagg ggtccgagcc ggagggcgct accgtcccgc caggaatccg ccaccggcga
     2161 ggagacgttc gaacccctcg gccggcgccg gaggcgcgaa gaggaatccc tgcccgaggt
     2221 cgcagcccat ctcccggagg ctccgcgccg tggcggcgtc ctccaccccc tcggcgacga
     2281 ccccgaggtc gaggctgtgg gcaagctgga ccgtcgagcg gacgacggcg agatccttgc
     2341 cgcccgccgc catcccggcg acgaaggacc ggtcgatctt caggacgtcg acggggagcc
     2401 gtgtgaggta ggcgagggaa gagtaccccg tgccgaagtc gtcgatcgcg agccgggccc
     2461 ccgagtcgtg cagccggccg agcatcttca gcgccgtctc cgggtcggac atgacggccg
     2521 actccgtcag ctcgaactcc acgagccccg ggtcgacgcc caccgacctc acgatctcct
     2581 ccagcttctc cggacagctc cggtcgacga ggttcctcgc ggagaggttc acggccatcg
     2641 tcagcttcgg cagctgacgg ttccagcggg agagctgctc gagcgcccgc cggacgaccc
     2701 acgaggtgag ggcgaccatg ccgtcggtcg cctcggcgaa cggcacgaac tggctcgagc
     2761 cgaggagccc gagccgcggg tgccgccacc ggacgagcgc ctcgaatccc tcgatgaagt
     2821 cgtactggag cgccactttc ggctggaaat ggaggacgag ctgcccctcc cgaatggccc
     2881 tcccgaggtc cgagaggatg gacagccgct cgggcgtgtg ctcgtcgagg tcgggctcgt
     2941 agcaggcgac gccgccggaa cccttcttcg cgacgtgcag cgccacgtcg gcgcagcgga
     3001 ggagtccgtg gctcacgcgg gcgtgggcgg ggtagtgcgc gaccccggcg ctcgcgccga
     3061 gctcgagccc catccccgcg acccggaacg gctcgccgag ccaggcgagg accttccggg
     3121 cggccgcctc gccatcctcc cggcgctccg cccccggcac gacgatcgcg aactcgtcgc
     3181 ccccgagccg gaagacctcc atgccgtcgg cgcacctctc cgcgaggcgc gcgccgacct
     3241 ggacgaggag ctcgtcgccg aagttgtgcc cgagcgtgtc gttgatctcg cggaaccagt
     3301 cgaggtcgag gaggacgagc gccgcccggc cgcctccggc cgtttcggcc agcgtcccga
     3361 agcgccggtg gaacccggcc cggttcggga ggccagtgag cgcgtcccgg aaggcccgct
     3421 cctcgagctc gctccggtgt cccgcgttcg cgaccgccag cgagaccgca cgcgcgacac
     3481 cttcgaacgt ctcggcctcc tcgggccgga gcggccgtcc ctcgggaaag atgacgttca
     3541 ccgtcccgag aggccgcccc ccgtgcgcga gcgggatgca gagcccggag gcgacgtggc
     3601 gccgggcgag cgcgtccagg accggatgca cctcgcgccc ctcgatgcgg acctcgccga
     3661 gcgagacgat ccgccgctcc cggacggccc gcccgctcgc gctcccctcg agcgggagcc
     3721 gggcgccgag gacgagctcc tcgtcggtga agccgcgccc cgccacgagc cg
//
LOCUS       DAOTEZ010000422         3771 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_18120_length_3771_cov_4.675996, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000422 DAOTEZ010000000
VERSION     DAOTEZ010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18120_length_3771_cov_4.675996"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2320)
                     /locus_tag="P5164_17110"
     CDS             complement(<1..2320)
                     /locus_tag="P5164_17110"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRY45655.1"
                     /translation="MRDLEPRQRRIVLFALGAALVVSLAWTTWLAHIGVERGALLRAR
                     LWFALVVFGVHYLGAVVVLLKRPFDPANQALFPFLTLLGVLNAGRILGVVTGESSLQD
                     RVAFAGSTAVHFVAIAWILVTIGGFFPVTRLHRPGRAYFVGTLLAMGPLALYLRRWWL
                     DGGGAAPGGAWRTGVALVALAVDLFVFVYFLSLAWVARKEDVRRRARVVLVAVLFYLG
                     LYIFLRDVPAVAGRGALLPVPALSGLSILLPLALIVAATRFRLFEVDRLIRRSASYAI
                     ASGALVVLLLAGVPALTALVALVSPALQGTLTTALVAVLLFLVFDPLRKRAQVALDRK
                     FRQGAYDPAQLVTDVVGDLRDAGEDLAGVVLHRIASALRPDRQAFWVFALEVPGAAVR
                     PVTTGALADVPGLDRAAVLPMSDPLESRNTFHALTADGGDPPYAEGELANGLRAGGFA
                     AALPLFSGDGEPLGLIALGAKRTGETYVPEEQRILGTVAGHVALALERAALTRQVDED
                     RRVREKVLGHLQEGGSGALYECELCGRVADEDDLVCTADGHAVKMTQAVPRLLAGRYR
                     LDKRLGEGGMGTVYAARDIRGNRDAAVKVIRAEHLADRSAVARFRREARLTARLGHPN
                     AVAVYDYGELRPGGAFLVMELLSGGSLRNELILRKPIPFLETAWALDRALDVLAAAHA
                     AGLVHRDVKPDNLFVTRGPGGTTRLKLLDFGLARETKAEGGAESGGAISSASILMGTP
                     GYIAPEIVSGAEATPASDQWAIAATGVELLTGV"
     gene            complement(2332..>3771)
                     /locus_tag="P5164_17115"
     CDS             complement(2332..>3771)
                     /locus_tag="P5164_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45656.1"
                     /translation="IATSAALLVVGLGSPYPLAMGTVALSVVATASARTAGRLGFLAA
                     VAGGALVALAFVYAPYLRLARDFDAPVGLRSIEAHAADLLSILHTGVFAGPLRDLLDA
                     LLPIFPEGASALFPTLTALFVLSVGTILLRRHPRALPRPRSARHAARSLVPWLLLASL
                     SFAFALGPTIRFAGRVVVPGPYVLLTGLPGFSGMRGIHRWDQWFGLGVIVAATLVLGR
                     LLRALPARRGRLLLAVVVPLLALDVWTRPVPAQAVPAPSPFDADIRALPRDAIVAIWP
                     FERGTSERSWAEQLSHRRRVLNGFQTFPPPIHLWLDSFLSDKPFEAAFAAYTELGASA
                     IEVDLEAAPPGSGERLGTLLASGALPGVRAAKGSRSRLLLLLEPKAPILVDPRTIEGL
                     RFDGDAAVVARPEGRLAFRLGSERLPVVVETSAGPVSDVLRIPVVGAGELSARLSFAP
                     PPGAVVRSRDGGHEIGRAASAAAAPPAGG"
BASE COUNT          637 a         1304 c         1445 g          385 t
ORIGIN      
        1 gcaccccggt cagaagctcc acgccggtcg ccgcgatcgc ccactggtcc gaagcggggg
       61 tcgcctccgc gccggagacg atctcgggag cgatatagcc gggcgtcccc atcaggatgc
      121 tcgcggagga gatcgcccct ccgctctcgg cccctccctc cgccttcgtc tcccgcgcga
      181 ggccgaagtc gaggagcttc agacgcgtcg ttccccccgg cccgcgagtg acgaagaggt
      241 tgtcgggctt gacgtcccgg tggacgagac cggccgcgtg ggccgccgcg aggacgtcga
      301 gcgcgcggtc gagcgcccag gccgtctcga ggaaggggat cggcttccgg aggatcagct
      361 cgttccgaag cgatccaccg gacaggagct ccatcacgag gaacgcgccg ccgggccgga
      421 gctcgccgta atcgtagacg gcgacggcgt tcgggtgccc gaggcgggcc gtcaaccggg
      481 cctcccgccg gaagcgggcg acggcgctcc ggtcggcgag gtgctcggcc cggatcacct
      541 tcaccgccgc gtcgcgattg ccccggatgt cccgcgcggc gtagacggtc cccatccctc
      601 cctcgccgag ccgcttgtcg agccggtagc gcccggcgag aagtcgaggc acggcctgcg
      661 tcatcttcac cgcgtggccg tcggccgtgc agacgaggtc gtcctcgtcc gcgacccggc
      721 cgcagagctc gcactcgtag agcgccccgc tccctccctc ctggagatgc ccgagcacct
      781 tctcgcgaac gcgacggtcc tcgtccacct gccgggtcag ggccgctctc tcgagggcga
      841 gggcgacgtg cccggcaacg gtcccgagga tccgctgctc ctcgggaacg tacgtctctc
      901 ccgtccgctt tgcgccgagc gcgatcaggc cgagcggctc cccatcgccc gagaaaagcg
      961 gcagcgccgc ggcgaatccg ccggcccgga ggccgttcgc gagctcgcct tcggcgtagg
     1021 gcgggtcccc tccgtcggcc gtgagggcat ggaacgtgtt ccgcgactcc agcgggtccg
     1081 acatcgggag gacggccgcc cggtccagcc cggggacgtc cgcgagggcg cccgtcgtca
     1141 ccggccgcac ggcggccccc ggcacttcga gcgcgaagac ccagaatgcc tgtcggtcgg
     1201 gccgaagcgc ggacgcaatc cggtggagga cgacgcccgc caggtcctcc ccggcgtcgc
     1261 gaaggtcccc gacgacgtcg gtcacgagct gcgccggatc gtacgcgccc tgccggaact
     1321 tccggtcgag cgcgacctga gccctcttgc gaagcgggtc gaagacgagg aagaggagca
     1381 cggcgacgag agccgtcgtg agcgtcccct gcagggcggg cgagacgagg gcgacgagcg
     1441 cggtgagcgc cggcaccccc gccaggagga ggacgacgag ggcgcccgag gcgatcgcat
     1501 acgaggcgct gcgacggatg agccggtcga cctcgaagag gcggaagcgc gtggccgcga
     1561 cgatgagggc gagcggcagg aggatcgaga gcccggagag ggccggcacc ggcaggagcg
     1621 cgcctcgtcc ggcgacggcg gggacgtcgc gcaggaagat gtagaggccg agatagaaga
     1681 ggacggcgac gaggacgacc cgtgcacgcc tccgcacgtc ctccttgcgc gcgacccacg
     1741 ccagggaaag gaagtacacg aagacgaaga ggtcgaccgc gagcgcgacg agcgcgacgc
     1801 cggtcctcca ggcgccgccg ggcgccgccc cgccgccgtc gagccaccac cgccgcaggt
     1861 agagggcgag gggccccatg gcgaggagcg tgccgacgaa gtaggcccgc cccggcctgt
     1921 gaaggcgcgt caccggaaag aacccgccga tcgtcacgag gatccaggcg atcgcgacga
     1981 aatggacggc cgtcgacccc gcgaaagcga ccctgtcctg cagcgaggac tcgccggtga
     2041 cgacgcccag aatcctcccc gcgttcagga cccccaggag cgtcaggaag gggaacaggg
     2101 cctggttcgc cgggtcgaag gggcgcttga ggaggacgac gacggccccg aggtagtgga
     2161 cgccgaagac cacgagcgcg aaccagagcc gggcgcgaag gagcgctcct ctctccaccc
     2221 cgatgtgggc gagccaggtc gtccacgcca gcgagaccac gagggccgcc ccgagggcga
     2281 agaggacgat ccgcctctgt cgaggctcga ggtcacgcac gggctcgggg attatccgcc
     2341 ggccggcgga gcggccgccg ccgatgcggc gcggccgatc tcgtgcccgc cgtcgcgcga
     2401 gcgtacgacg gcgccgggcg ggggtgcgaa ggagaggcgc gcgctcagct cgcccgcgcc
     2461 gacgaccgga atgcggagga cgtcggaaac ggggcccgcg gacgtctcga ccacgaccgg
     2521 aagccgctcg ctcccgaggc ggaaggcgag ccgcccctcc ggcctggcga ccacggcggc
     2581 gtccccgtcg aatcgaagtc cctcgatcgt ccgaggatcg acgaggatcg gagccttcgg
     2641 ttcgaggagg aggagaagcc gcgaccggct gcccttcgcg gcccggaccc ccggaagcgc
     2701 ccccgaagcc aggagcgtcc cgagccgctc gcccgacccg ggcggcgccg cttcgaggtc
     2761 gacctcgatc gcggaagcac cgagctcggt gtaggccgcg aaggccgcct cgaagggctt
     2821 gtccgagagg aaggagtcga gccagaggtg gatcggcgga gggaaggtct ggaagccgtt
     2881 caggacgcgg cggcggtgag agagctgctc cgcccaggag cgctccgagg taccacgctc
     2941 gaagggccag atcgccacga tggcatcgcg cggcagggca cgaatgtccg cgtcgaacgg
     3001 cgacggcgcc gggaccgcct gggccggcac gggcctcgtc cagacgtcga gggcgaggag
     3061 cgggacgacg accgcgagga ggaggcggcc gcgtcgcgcg gggagcgccc ggaggagccg
     3121 gccgaggacg agggtcgccg cgacgatcac gcccaggccg aaccactggt cccaccggtg
     3181 gatcccgcgc attccgctga aaccagggag ccccgtgagg agcacatacg gtccggggac
     3241 gacgacgcgg cccgcgaatc ggatcgtcgg gccgagcgcg aaggcgaagg aaaggctggc
     3301 gaggagaagc cacgggacca gcgaccgggc ggcgtgacgc gcgctccgtg gccgcgggag
     3361 cgcccgagga tgacgacgga gaaggatcgt tccgacggag agaacgaaca gggcagtaag
     3421 ggtcggaaag agcgccgagg ccccttcggg gaagatcggc aggagcgcgt cgagcaggtc
     3481 ccggagcggc cccgcgaaga cccccgtgtg gaggatcgag aggaggtcgg ccgcgtgggc
     3541 ctcgatcgac cggaggccga cgggagcgtc gaagtcgcgg gcgaggcgga ggtagggggc
     3601 gtagacgaag gcgagcgcga cgagcgcccc gccggcgacg gccgcgagga agccgagcct
     3661 gccagcggtc cgggccgacg cggtcgcgac gacggagagc gcgaccgttc ccatcgcgag
     3721 cggatatggg ctcccgagac cgacaacgag aagggccgcg gacgtcgcga t
//
LOCUS       DAOTEZ010000423         3767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_18142_length_3767_cov_6.452856, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000423 DAOTEZ010000000
VERSION     DAOTEZ010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18142_length_3767_cov_6.452856"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2728
                     /locus_tag="P5164_17120"
     CDS             <1..2728
                     /locus_tag="P5164_17120"
                     /inference="COORDINATES: protein motif:HMM:NF026115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY45657.1"
                     /translation="QAELFSAVREEYGIPRDDGRKLRDYPTLGHVVRFVYDNRPDLAA
                     GGTAAAPAAPAPDGPSASVAPAASRSPLGSFAEAAKVPRRVVVPVVRPALPACRATGV
                     ALGAGARVAVMFDRGGAAEALVERLRSRGADVLTLAHGASADALAERLAEWTAGGPVH
                     GLFWLPALDAEGDLRAMDLAAFREALRVRVKSLYTTARALYGALGSPGTFLVSATRLG
                     GRHGYDAAGAVAPLGGAVSGFTKAFKREKAEATVKVVDFGVDAAPSVVADLLIEEALR
                     DPGCVEVGYADGLRTSVGMRELPVEDGTPGMPLGKESVFLVTGAAGSIVSAITTDLAA
                     ASGGTFHLLDLLPEPDRNDPDLARVATDREGLKKDVFERLKAKGERATPALVERELTR
                     LERLAAALEAIRAVEAAGGTVHWYAGDLRDAARVREIVSDVLARSPRVDVLLHGAGLE
                     ISRFLADKEPKEFDLVFDVKADGFFNLLQALGDTPIGATVAFSSVAGRFGNGGQTDYS
                     SANDLLCKLSSSFRTTRPGTRGIALDWTAWGDIGMATRGSIPKMMELAGIDMLPAASG
                     IPIIRRELTSSSFAGEIVEGLRLGILTKEWDETGGLDPAAAPALPAGPMTGRLAAFRL
                     HSGLLVETELDPKAQPFLFDHRIDGTAVLPGVMGIEAFAETAAALLPGFRVASVEDVD
                     FLAPFKLYRDEPRSVAVEAVLRPAGDAVEAECRLVGRRTLPGQAEPQETVHFRARVRL
                     ERGETAPETFAAALENPGAPLTAADIYSVYFHGPAYQVLESAWKNGSGVVGLFADPLP
                     PNHAPDAPTVVAPRLVELAFQTAGVFELGTAGRMGLPLRVERVRPVRPLEAGSGRLHA
                     VVTPSASGDGTFDARVVDANGNVHLVVEGYRTVELPGGAPADRLAPFRAAMA"
     gene            2795..>3767
                     /locus_tag="P5164_17125"
     CDS             2795..>3767
                     /locus_tag="P5164_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HRY45658.1"
                     /translation="MNPDFQRVAIVNRGEAAMRFINAVREFNQEHGTAIRTVALYTDP
                     DRRAMFVREADEAYSLGPATVSSGDGERKVGYLDYARLETALAGAGVDAVWTGWGFVS
                     EHAGFAELCDRLGIVFIGPPPDAMRRLSDKVAAKTLAAEAGVPVVPWSGTSVAGPAEA
                     AEAATALGYPVVVKAAAGGGGRGIRVVGSPEEMPAAFESARNEAYRAFGDATVFVEKL
                     LLGVRHVEVQVLGDSWGTTWAVGVRDCTVQRRHQKVLEESPAPTLSDETVEELKTAAL
                     RIARTAGYRNAGTVEFLFDPKDATFAFLEVNARLQVEHTVTEVTTGLD"
BASE COUNT          466 a         1469 c         1324 g          508 t
ORIGIN      
        1 gcaggccgag ctcttcagcg cggtgcgcga ggagtacggc atcccgcgcg acgacggccg
       61 caagctccgc gactacccga cgctcggcca cgtcgtccgc ttcgtctacg acaaccggcc
      121 cgacctcgcc gccggcggga cggccgccgc gccggccgct ccggcgccgg acggcccgtc
      181 tgcgtcggtc gcgcccgccg cgtcccgatc gccgctcggc tctttcgccg aggcggcgaa
      241 ggtgccgcgc cgggtcgtcg tccccgtcgt ccgtcccgcc ctgccggcct gcagggcgac
      301 cggcgtcgcg ctcggcgccg gcgcgcgcgt cgccgtcatg ttcgaccgcg gcggcgccgc
      361 cgaggcgctc gtcgagcggc tccgctcccg cggcgccgac gtcctgacgc tcgcgcacgg
      421 cgcctccgcc gacgcgctcg ccgagcgcct cgccgagtgg accgcgggcg gccccgtcca
      481 cggtctcttc tggctcccgg cgctcgacgc cgagggagac cttcgcgcga tggacctcgc
      541 ggctttccgc gaggcgctcc gcgtgagggt caagagcctc tacaccaccg cccgggcgct
      601 ctacggcgcc ctcggctctc ccggcacgtt cctcgtctcg gcgacccgtc tcggcggccg
      661 gcacggctac gacgccgccg gcgccgtcgc gccgctcggc ggcgcggtct ccggcttcac
      721 gaaggccttc aagcgcgaga aggccgaggc gaccgtgaag gtcgtcgatt tcggcgtcga
      781 cgccgcgccg tccgtcgtcg cggacctcct gatcgaggag gcgcttcgcg acccgggctg
      841 cgtcgaggtc ggctacgccg acggcctccg gacctccgtc ggcatgcggg agctccccgt
      901 cgaggacgga acgcccggga tgccgctcgg caaggagagc gtcttcctcg tcacgggcgc
      961 ggcggggagc atcgtctcgg cgattacgac cgacctcgcc gccgcctcgg gcggcacgtt
     1021 ccacctcctc gacctcctcc ccgagccgga ccgcaacgat cccgacctcg cgcgcgtcgc
     1081 gaccgaccgc gaaggactga agaaggacgt cttcgagcgc ctgaaggcga agggggagcg
     1141 cgccacgccg gccctcgtcg agagggagct gacgcgactc gagcggctcg ccgccgccct
     1201 cgaggcgatc cgcgcggtcg aggccgcggg cggcaccgtc cactggtacg cgggcgacct
     1261 ccgcgacgcg gcgcgcgtgc gggagatcgt ctccgacgtc ctcgcgcgga gcccgcgcgt
     1321 cgacgtcctt ctccacgggg cggggctcga gatcagccgt ttcctcgccg acaaggagcc
     1381 gaaggagttc gacctcgtct tcgacgtgaa ggccgacggc ttcttcaacc tcctgcaggc
     1441 gctcggcgac acgcccatcg gcgccaccgt cgccttctcc tcggtcgccg ggcgcttcgg
     1501 caacggcggc cagaccgact acagctccgc gaacgacctc ctctgcaagc tctcctcgag
     1561 cttccggacg acgcgccccg gcacgcgcgg gatcgcgctc gactggaccg cctggggcga
     1621 catcggcatg gcgacccgcg gctcgatccc gaagatgatg gagctggccg gcatcgacat
     1681 gctccccgcc gcctcgggca tcccgatcat ccggcgcgag ctgacgagct cttccttcgc
     1741 gggggagatc gtcgaagggc tccggctcgg catcctgacg aaggagtggg acgagacggg
     1801 cggcctcgac ccggcggcgg cgccggccct cccggccggc ccgatgacgg ggcgcctcgc
     1861 ggcgttccgc ctccattccg gcctcctcgt cgagacggag ctcgacccga aggcgcagcc
     1921 cttcctcttc gaccaccgca tcgacggcac cgccgtcctg ccgggcgtga tggggatcga
     1981 ggcgttcgcc gagacggccg ccgcgctcct ccccggcttc cgggtcgcgt ccgtcgagga
     2041 cgtcgacttc ctcgccccct tcaagctcta ccgcgacgag cctcgctccg tcgccgtcga
     2101 ggcggtcctt cgcccggcgg gcgacgccgt cgaggccgag tgccgcctcg tcggtcgccg
     2161 gaccctgccg ggacaggccg agccgcagga gacggtccac ttccgggcgc gcgtccgcct
     2221 cgaacgaggc gagacggcgc ccgagacctt cgccgccgcg ctcgagaacc ccggcgcgcc
     2281 cctcaccgcc gccgacatct actccgtcta cttccacggt ccggcctacc aggtgctcga
     2341 gagcgcctgg aagaacggct ccggcgtcgt cggcctcttc gccgacccgc ttccgccgaa
     2401 ccacgccccc gacgcgccga ccgtcgtcgc gccgcgcctc gtcgagctcg ccttccagac
     2461 ggccggcgtt ttcgagctcg gcacggccgg gcggatgggc ctgccgctgc gcgtcgagcg
     2521 ggtccgcccc gttcggcccc tcgaggccgg gagcggccgg cttcacgccg tggtgacgcc
     2581 gtcggcctcg ggcgacggga ccttcgacgc ccgcgtcgtc gacgcgaacg ggaacgtcca
     2641 cctcgtcgtc gaggggtacc ggacggtcga gctgccgggg ggagccccgg ccgaccggct
     2701 cgcgccgttc cgcgccgcga tggcctgaga cgtccctttc tccccggagg gcggtcctcg
     2761 gaccgccctc cgggggttcc ggtctggagg agagatgaat cctgatttcc agcgggtcgc
     2821 catcgtcaac cgcggcgagg cggcgatgcg cttcatcaac gccgtccggg agttcaacca
     2881 ggagcacggg acggcgatcc ggacggtcgc cctctacacc gaccccgacc ggcgcgcgat
     2941 gttcgtgcgc gaggccgacg aggcgtactc cctcggaccg gcgacggtct cctcgggaga
     3001 cggcgagcgg aaggtcgggt acctcgacta cgcccgcctc gagaccgccc tcgccggcgc
     3061 gggggtggac gccgtctgga caggctgggg cttcgtctcc gagcacgccg gcttcgcgga
     3121 gctctgcgac cggctcggga tcgtcttcat cggcccgccg ccggacgcga tgcggaggct
     3181 gagcgacaag gtcgccgcga agacgctcgc cgccgaggcg ggcgtcccgg tcgtcccgtg
     3241 gagcgggacc tcggtcgccg gacccgcgga ggcggcggag gccgcaaccg cactcggcta
     3301 cccggtcgtc gtcaaggccg ccgccggcgg gggaggacgc gggatccgcg tcgtcggttc
     3361 cccggaggag atgcccgccg cgttcgagag cgcgcggaac gaggcgtacc gcgcgttcgg
     3421 cgacgcgacc gtcttcgtcg agaagctcct tctcggcgtg cggcacgtcg aggtgcaggt
     3481 cctcggcgac tcctggggga cgacctgggc cgtcggcgtc cgggactgca ccgtccaacg
     3541 gcgtcaccag aaggtcctcg aggagtcgcc cgcgccgacg ctctccgacg agaccgtcga
     3601 ggagctgaag accgccgcgc tccggattgc ccgcacggcc ggataccgga acgccggcac
     3661 ggtcgagttc ctcttcgacc cgaaggacgc cacgttcgca ttcctcgagg tgaacgcgcg
     3721 cctgcaggtc gagcacaccg tcaccgaggt gacgaccggc ctcgacc
//
LOCUS       DAOTEZ010000424         3764 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_18158_length_3764_cov_5.224050, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000424 DAOTEZ010000000
VERSION     DAOTEZ010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18158_length_3764_cov_5.224050"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..89
                     /locus_tag="P5164_17130"
     CDS             <1..89
                     /locus_tag="P5164_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014920852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY45659.1"
                     /translation="VVSIPVVALFLFLQKYLVSGLTAGSVKG"
     gene            116..928
                     /locus_tag="P5164_17135"
     CDS             116..928
                     /locus_tag="P5164_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HRY45660.1"
                     /translation="MKRWLFPTFVLLAVALVAGTAFAQEVSFKDPTGDDKGPGTYVYP
                     TDKVYKAGSFDLVEFKMKASGDKATFSVTLGSALEDPWGMGGGFATQMVFVFIDTDGK
                     EGSGFTKGLPGLNVAFAPADAWDKCVILSPQPQNRVVSEVETKAAWAKGAVLVPTRTR
                     GAGRTISGTVSLKELGEGDPAKWGYQVVVQSNEGFPDKADLLTRKVNEFEGQHRFGGG
                     TDGDCDPHVMDILAGKGTGDAAEVEEQKKALAYECEADGTPKKTATLPMVRK"
     gene            968..3163
                     /locus_tag="P5164_17140"
     CDS             968..3163
                     /locus_tag="P5164_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45661.1"
                     /translation="MRTTKTLGLVLLLVAVAVSGTAWAQQGPQLTISGTTYTKWLWGN
                     SRDQGALYNFKDAPGEGYGDNGQGTEIELLLNAKVSKAVEVNARIHSRFYQNFWTNMG
                     GWGKPSDDCSGSGTSVGCGEFDPRSNQYIKLRGVTVTLTPGYSWLDSATIGSSDWGMF
                     DPYVVGRIRYIDRDNVQGLLFQGSAMDKALTWDAARISLPRLWAGPEYKTGNFHVADG
                     AYVGQFKYSGGSTWDIGGLVQWVNDIEVDSRDINWDDGKDTRTRFRNTVFGAKLGIHP
                     SAAVDAKAAFYYSTYESASDLAPASFGMNGYSPVPAGQHNDWSGKLNLDFADLGGTGF
                     GVNVEAFHIGAEYASAMAARRESDVLLTEGHDSTWALPGPGNATYGIWGGNPTVIGYG
                     GWQGNAQQVATINVDNQFTDFNEPMAETAIGWEGITITPTFQSGNLELAGEYTYLTYD
                     TNWQAWGDDSRAITNSPFPAMDMQVGMASYRSAYAPFQDKTTHIALLKGKYVIEAGKG
                     IDLFGKIKLISETDKRLNDAKYLPYLPGDCATSGTLGCKNQVNKYNGTNSTADFFNNP
                     GVITGANGQVGYQWKPFSDVSDDDRDLSYVMVQAGAGYQWTRDLYGSLEYIYYDADLE
                     DGNTAFQAYNMMEMASGKHQKNQLIAKFRYTLGGLSECGLVYEYNFGTFKPDYGDGFV
                     PTTASEETARNVHVPVGSLGFFNRYGGWNTMEKREFDQQRLKAYLKILF"
     gene            3259..>3764
                     /locus_tag="P5164_17145"
     CDS             3259..>3764
                     /locus_tag="P5164_17145"
                     /inference="COORDINATES: protein motif:HMM:NF021500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HRY45662.1"
                     /translation="MTSRPPCLLRGAAVLAALLPLFSPRTAAGAAEGALFVVADPEKD
                     DHGDGHLTYPVRSRNDMLPGHLDLVSFAARREGDGTMFEATFARPVVKTEKVPLDGGG
                     TLMTDVMRLGFYTFNVDVYIDVDRVPGSGNTETLPGRNAEVDPAGAWEKAICLTPLPG
                     PAETLLRR"
BASE COUNT          682 a         1347 c         1211 g          524 t
ORIGIN      
        1 tcgtcgtctc catccccgtc gtcgccctgt tcctcttcct ccagaagtac ctcgtctccg
       61 gcctcacggc cgggagcgtc aagggctgaa tccaaagcag aaagggaggc tccaaatgaa
      121 gagatggctt ttcccgacgt tcgttctcct cgccgtcgcc ctcgtggccg gcaccgcctt
      181 cgcccaggag gtctccttca aggacccgac gggcgacgac aagggaccgg gcacctacgt
      241 ctacccgacc gacaaggtct acaaggccgg atcgttcgac ctcgtcgagt tcaagatgaa
      301 ggcctcgggc gacaaggcca ccttctcggt cacgctcggc agcgccctcg aggacccgtg
      361 ggggatgggc ggcggcttcg cgacccagat ggtcttcgtc ttcatcgaca ccgacggcaa
      421 ggaggggagc ggcttcacga agggcctccc cggcctgaac gtcgcgttcg cgcccgccga
      481 cgcgtgggac aagtgcgtga tcctctcgcc gcagccccag aaccgcgtgg tcagcgaggt
      541 cgagacgaag gccgcctggg ccaagggcgc cgtcctcgtc ccgacgcgga cgcggggcgc
      601 cggccgaacg atcagcggca ccgtgagcct caaggagctc ggcgaaggcg acccggcgaa
      661 gtggggctac caggtcgtcg tccagtcgaa cgaggggttc ccggacaagg ccgacctcct
      721 cacccggaag gtgaacgagt tcgaggggca gcaccggttc ggcggcggga ccgacggcga
      781 ctgcgacccg cacgtcatgg acatcctcgc gggcaagggg accggcgacg ccgcggaggt
      841 cgaggagcag aagaaggcgc tcgcctacga gtgcgaggcg gacggtacgc cgaagaagac
      901 ggcgaccctc ccgatggtcc gcaagtaggc gcgtcccgtt ccgattcgaa ggaaggagag
      961 tagagacatg cgcacgacga agaccctcgg gctcgtgctg ctcctcgtgg ccgtggccgt
     1021 ctccggcacg gcctgggcgc agcagggccc gcagctcacg atcagcggca cgacctacac
     1081 gaagtggctc tggggcaaca gccgggacca gggggccctc tacaacttca aggacgcccc
     1141 cggcgagggc tacggcgaca acggccaggg aacggagatc gagctcctcc tgaacgcgaa
     1201 ggtctcgaag gccgtcgagg tcaacgcccg catccacagc cgcttctacc agaacttctg
     1261 gacgaacatg ggcggctggg gcaagccgtc ggacgactgc agcggctcgg gcacctcggt
     1321 cggctgcggc gagttcgacc cgcgctcgaa ccagtacatc aagctccgcg gcgtcaccgt
     1381 gacgctgacc ccgggctact cctggctcga ctcggccacg atcggctcga gcgactgggg
     1441 gatgttcgac ccgtacgtcg tcggccgcat ccgctacatc gaccgcgaca acgtccaggg
     1501 gctcctcttc cagggctccg cgatggacaa ggctctcacg tgggacgcgg cgcgaatctc
     1561 cctcccgcgt ctctgggccg gccccgagta caagacgggg aacttccacg tcgccgacgg
     1621 ggcgtacgtg ggccagttca agtactccgg cggctcgacg tgggacatcg gcggcctcgt
     1681 ccagtgggtg aacgacatcg aggtcgactc ccgcgacatc aactgggacg acggcaagga
     1741 cacgcggacg cggttccgca acaccgtctt cggcgcgaag ctcggcatcc acccgagcgc
     1801 cgccgtcgac gcgaaggccg ccttctacta ctcgacctac gagtcggcga gcgacctcgc
     1861 ccccgcgagc ttcggcatga acggctactc gccggtcccc gcgggccagc acaacgactg
     1921 gagcgggaag ctgaacctcg atttcgccga cctcggcggc accggcttcg gcgtcaacgt
     1981 cgaggcgttc cacatcggcg ccgagtacgc gtctgccatg gcggcccgtc gcgagtcgga
     2041 cgttctcctc accgaggggc acgactccac ctgggcgctc ccgggccccg gcaacgcgac
     2101 ctacggcatc tggggcggca acccgaccgt catcggctac ggcggctggc aggggaacgc
     2161 ccagcaggtc gcgacgatca acgtcgacaa ccagttcacc gacttcaacg agccgatggc
     2221 cgagaccgcg atcgggtggg aggggatcac gatcaccccg accttccaga gcgggaacct
     2281 cgagctcgcc ggcgagtaca cctacctcac ctacgacacg aactggcagg cctggggcga
     2341 cgactcccgg gcgatcacga actcgccctt cccggcgatg gacatgcagg tcggcatggc
     2401 gagctaccgc tcggcctacg cccccttcca ggacaagacg acgcacatcg cgctcctcaa
     2461 gggcaagtac gtcatcgagg cgggcaaggg gatcgacctg ttcggaaaga tcaagctcat
     2521 cagcgagacc gacaagcggc tcaacgacgc gaagtacctc ccgtacctcc cgggagactg
     2581 cgcgacgtcc gggacgctcg gctgcaagaa ccaggtcaac aagtacaacg ggacgaactc
     2641 gacggccgac ttcttcaaca accccggcgt gatcaccggc gcgaacgggc aggtcggcta
     2701 ccagtggaag ccgttcagcg acgtctccga cgacgaccgg gacctgagct acgtgatggt
     2761 ccaggccggc gccgggtacc agtggacgcg cgacctctac ggctccctcg agtacatcta
     2821 ctacgacgcg gacctcgagg acggcaacac cgccttccag gcctacaaca tgatggagat
     2881 ggcctcgggc aagcatcaga agaaccagct catcgcgaag ttccgctaca cgctgggcgg
     2941 cctctccgag tgcggcctcg tctacgagta caacttcggc acgttcaagc ccgactacgg
     3001 cgacggcttc gtcccgacga cggcgagcga ggagaccgcc cggaacgtcc acgtcccggt
     3061 cggctccctc ggcttcttca accgctacgg cggctggaac accatggaga agcgcgagtt
     3121 cgaccagcag cggctgaagg cctacctcaa gatcctcttc taggaccccg tcccgcaagg
     3181 accgaagggc ccggcagctt cggctgccgg gccctttttt cctgctccga cccccgcctc
     3241 ctgcggcaag atcccggcat gacctcccgc ccgccgtgcc tcctccgggg cgccgccgtg
     3301 ctcgccgcgc tcctccctct cttctccccg cgaacggccg ccggcgcggc cgagggcgcg
     3361 ctcttcgtcg tggccgatcc cgagaaggac gaccacggcg acgggcacct cacctatccg
     3421 gtccggagcc ggaacgacat gctccccggc cacctcgacc tcgtctcctt cgccgcccgg
     3481 cgcgaagggg acgggacgat gttcgaggcc accttcgccc gccccgtcgt gaagacggag
     3541 aaggtgccgc tcgacggggg cgggacgctg atgaccgacg tcatgcgcct cggcttctac
     3601 accttcaacg tcgacgtcta catcgacgtc gaccgggtcc cgggctccgg aaacaccgag
     3661 acgctcccgg gccggaacgc cgaggtcgac ccggccggcg cctgggagaa ggcgatctgc
     3721 ctcaccccgc tccccgggcc cgccgagacg ctgctccggc ggag
//
LOCUS       DAOTEZ010000425         3763 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_18167_length_3763_cov_6.215750, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000425 DAOTEZ010000000
VERSION     DAOTEZ010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18167_length_3763_cov_6.215750"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..595)
                     /gene="atpH"
                     /locus_tag="P5164_17150"
     CDS             complement(35..595)
                     /gene="atpH"
                     /locus_tag="P5164_17150"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HRY45663.1"
                     /translation="MRAAGAAFTRPYVDALFEVAGSADAVEALLPSLETVARALDASE
                     ELRAFSTNPAIPRAVKRALVASLAGNAGAPELAGRLLRTLLDRGRVLALSAVLDAIRE
                     RLDEARGVVEATVRSVAPLGADAETAIREALEARSGTRVRLRTELDPSLLSGFVVRLG
                     SEVFDASLSRRLARARKALEGASGAA"
     gene            complement(592..1134)
                     /gene="atpF"
                     /locus_tag="P5164_17155"
     CDS             complement(592..1134)
                     /gene="atpF"
                     /locus_tag="P5164_17155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRY45664.1"
                     /translation="MSSGPIALLLAGGGEGAHTFLGLPYPFWQTLNLLGFLGLLVWLL
                     RKPLVQFFENRRTQVAESLARAEADRDRAEAVAKEVGERLTRIEAEIEAMRRNALEQA
                     RAEESEIEARAAEEAARISARTAAELDARVRTARNDLTAYAADLAVELARELVAKNVT
                     PDDEKRLVAEGVKNLAGEGR"
     gene            complement(1134..1562)
                     /locus_tag="P5164_17160"
     CDS             complement(1134..1562)
                     /locus_tag="P5164_17160"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HRY45665.1"
                     /translation="MQLPDLSLILVVVIFWAAYVVLRRFLLAPLGAVLAARERRVDEA
                     RASSEAALARQREVAADLDRRLTEARREALAHREAVRAAAGERRQAILDEARENARSA
                     AAQAQARLERDALAAREQLAAEAKAIAAEIANYALGRKVA"
     gene            1750..2370
                     /locus_tag="P5164_17165"
     CDS             1750..2370
                     /locus_tag="P5164_17165"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRY45666.1"
                     /translation="MISAFEIASRLWDEASTRTAPGEVACRAGCFGCCIGLFEISLAE
                     AVLVRSGVASLPDEVRADVLSRARRIVAESARAFPGDAERGLLDPDRTEAADDAYFEV
                     VADRACPMLELPSGRCRIYAARPITCRTYGFAWAKDGAMIHPPCGLNLPGASAGRQLQ
                     ASIDIGRIDEGLGVAGEIAEELDLDPDVESTIAHAVVGSAFGPRLG"
     gene            complement(2372..2788)
                     /locus_tag="P5164_17170"
     CDS             complement(2372..2788)
                     /locus_tag="P5164_17170"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRY45667.1"
                     /translation="MIGGKKGGVLNGFLDRGARFEGTLSFEDTFRIDGVFKGKVVTKN
                     ELVIGDGASIDAEIHVGRLSVSGNVRGVVYARERVEVHAGARLQAEIHTPSLLVEDGA
                     IIQGPVQTGPQVGRAPGPPSDPALPVAGVAAPTKKG"
     gene            complement(2785..3654)
                     /locus_tag="P5164_17175"
     CDS             complement(2785..3654)
                     /locus_tag="P5164_17175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRY45668.1"
                     /translation="MTKKPALGKGLSALIPTNRPAPAEPRPGSSVQMIDLDHLEANRR
                     QPRRRFDDDGIEELARSIKESGILQPILVTRDGERFRILAGERRARAARVAGLSRVPV
                     LVREGLEDRDHLLLALVENLQRRDLTAVEEAEGYQHLKDSFGMTQEEIAERVGKDRTT
                     VANAMRLLKLPAEIRTQVEDGLLTAGHVRPLLALSSAADQETLAREFLRRGLSVRAAE
                     ARVQEFLAGGPKKPAPPEGDPDTRDAERRLQRALGTKVEIRRRKKGGEVRLSFYSEEQ
                     LIGLFERLMGGEG"
BASE COUNT          487 a         1366 c         1367 g          543 t
ORIGIN      
        1 cattgcggcg tctctttcct cgtcctgtgg tgggtcacgc ggccccggag gccccctcca
       61 gagccttccg cgcccgagcg agccggcgcg aaagggaggc gtcgaagacc tcgctcccca
      121 gccggacgac gaagcccgac aggagcgagg ggtcgagttc ggtgcggagg cggacgcgcg
      181 tcccgctgcg cgcctcgagg gcctcgcgga tcgccgtctc ggcgtccgcg ccgagcggcg
      241 cgacggagcg gacggtcgcc tcgacgacgc cccgcgcctc gtcgaggcgc tcgcggatcg
      301 cgtccaggac ggcggagagc gccaggacgc gtccccggtc caggagcgtt cggaggagcc
      361 gcccggcgag ctcgggggct cccgcgttcc cggccagcga ggcgacgagc gcccgcttga
      421 ccgcgcgcgg gatcgccggg ttcgtggaga aggcccgaag ctcctcggag gcgtccaggg
      481 cgcgggcgac cgtctcgagg gacgggagga gcgcctctac ggcgtcggca gagccggcga
      541 cctcgaagag cgcgtcgacg taggggcgcg tgaaggcggc tccggccgct ctcatcgacc
      601 ctctccggcg aggttcttca cgccttcggc gacgagccgc ttctcgtcgt cgggcgtgac
      661 gttcttcgcg acgagctcgc gggcgagctc caccgcgaga tcggcggcgt acgcggtcag
      721 gtcgtttcgc gcggtcctca cgcgggcgtc cagctcggcc gcggtgcgcg ccgagatccg
      781 ggccgcttcc tcggcggcgc gggcctcgat ctcggactcc tcggcccggg cctgctcgag
      841 tgcgttccgg cgcatcgcct cgatctccgc ctcgattcgc gtgagccgct ccccgacctc
      901 cttcgcgacc gcttcggcgc ggtcccggtc cgcctcggcc ctggcgagcg attcggcgac
      961 ctgcgtgcgc cggttctcga agaactggac gagcggcttt cggaggagcc agacgagaag
     1021 cccgaggaac ccgaggaggt tcaacgtctg ccagaagggg tacgggaggc cgaggaaggt
     1081 gtgggctccc tcccctccgc cggcgaggag aagcgcgatc ggtccggacg acatcaggcc
     1141 accttccggc cgagggcgta gttcgcgatc tcggcggcga tcgccttcgc ctcggcggcg
     1201 agctgctcgc gggcggcgag cgcgtcgcgc tcgagccggg cctgcgcctg ggccgcggcg
     1261 ctccgcgcgt tctcgcgcgc ttcgtcgagg atcgcctgcc ggcgctcgcc ggcggcggcg
     1321 cggaccgcct cccgatgcgc gagcgcctcg cggcgcgcct cggtcagccg gcggtcgagg
     1381 tcggccgcga cctcgcgctg gcgcgcgagc gccgcctcgg acgaggcccg ggcctcgtcg
     1441 acgcgccgct cgcgcgccgc caggacggcg ccgagcggcg cgagcaggaa tctcctcagg
     1501 accacgtagg cggcccagaa gatgacgacg acgagaatca gggagaggtc gggcagttgc
     1561 atcgctcgtt ccgctctcga gggcgcgctg cggactccgc ccccgggcgc accgcgagcc
     1621 ggaagtccgc acgggggccg cgtttatgcc acagacccgc cggggcggtc aacgcgagca
     1681 cgccggccgc gctacgattc ccgccgggat gacggcgaag cggtgagaac agacaaaaca
     1741 accgaacgct tgatttcggc tttcgaaatc gcttcccggc tctgggacga ggcctcgacc
     1801 cggaccgcgc cgggagaggt cgcctgccgc gccggctgct tcggctgctg catcgggctc
     1861 ttcgagatct cccttgccga ggcggtgctc gtccgatccg gagtggcatc tctcccggac
     1921 gaggtccgcg cggacgtcct ctcgcgcgcc cgccggatcg tcgccgagag cgcccgagcg
     1981 ttcccgggcg acgcggagcg ggggctgctc gatcccgatc gcaccgaagc ggccgacgac
     2041 gcctatttcg aggtggtcgc cgatcgggcc tgcccgatgc tggagcttcc ttccggccgc
     2101 tgccgcatct acgccgcacg cccgatcacg tgccggacct acgggttcgc ctgggcgaag
     2161 gacggggcga tgatccatcc cccgtgcggg ctgaacctcc cgggcgcgtc tgccggaagg
     2221 cagctccagg cgtcgatcga catcggccgg atcgacgagg gcctgggcgt ggccggggag
     2281 atcgcggagg agctcgacct cgacccggac gtcgagtcga cgatcgcgca cgccgtcgtc
     2341 ggaagcgcct tcggcccccg cctcggctga ttcagccctt cttcgtcgga gccgccacac
     2401 cagcgaccgg cagggccggg tcggagggcg gccccggagc ccggccgacc tgcggtccgg
     2461 tctggacggg accctggatg atcgccccgt cctcgacgag aagcgacggg gtgtggatct
     2521 cggcctgaag acgcgcgccg gcgtggacct cgacccgctc ccgggcatag acgacgcccc
     2581 gaacgtttcc cgacacggag aggcgtccga cgtgaatctc ggcgtcgatc gacgccccgt
     2641 ccccgatgac gagctcgttc ttcgtcacga cctttccctt gaaaacgccg tcgatccgga
     2701 acgtgtcctc gaaggagagg gtcccctcga atcgggcccc cctgtcgagg aacccgttca
     2761 ggacgccccc cttcttcccg ccgatcaccc ttcccctccc atgaggcgct cgaagaggcc
     2821 gatcagctgc tcctccgagt agaacgagag gcggacctcg ccccccttct tccggcggcg
     2881 gatctcgacc ttcgtcccga gggcccgctg caggcggcgc tcggcgtccc tggtatcggg
     2941 atccccctcg ggaggggccg gcttcttcgg cccgccggcc aggaactcct ggacgcgggc
     3001 ctcggccgcg cggacggaga ggccgcgccg gaggaactcg cgggcgagcg tctcctggtc
     3061 cgcggccgag gagagggcga gcagcggccg gacgtggccc gcggtgagga gaccgtcctc
     3121 cacctgggtt cggatctcgg ccgggagctt caggaggcgc atcgcgttcg cgacggtcgt
     3181 cctgtctttc ccgaccctct ccgcgatctc ttcctgggtc atcccgaacg agtccttgag
     3241 atgctggtat ccctccgctt cctcgacggc cgtcaagtcg cgccgctgga ggttctcgac
     3301 gagggccagg aggaggtggt cgcgatcctc caacccttcc cggacgagca ccgggacgcg
     3361 ggagaggccg gccacccggg cggcccgggc gcggcgttcc ccggccagta tccggaaccg
     3421 ctccccgtcc cgggttacga ggatcggctg gaggatcccg gactccttga tcgagcgggc
     3481 cagctcctcg atgccgtcgt cgtcgaaccg gcgccggggt tgacggcggt tcgcctcgag
     3541 gtggtcgagg tcgatcatct ggaccgagct ccccgggcgg ggctcggccg gggccgggcg
     3601 attcgtgggg atgagggccg agaggccctt cccaagcgcg ggcttcttcg tcacggctgc
     3661 cctccgttcc cgccggccag gtcccgccgc aggatctctc gggcgatggc gaggtaggcc
     3721 tcggccccgc gcgagcggat gtcgtaaagg aagatcggct tgc
//
LOCUS       DAOTEZ010000426         3739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_18376_length_3739_cov_3.715255, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000426 DAOTEZ010000000
VERSION     DAOTEZ010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18376_length_3739_cov_3.715255"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="P5164_17180"
     CDS             complement(<1..585)
                     /locus_tag="P5164_17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HRY45669.1"
                     /translation="MTPAGLQTTLLAISRMDCPSEERLVRMALDGVEGVDDLRFDLRS
                     RTLSVTHRGEPGALLAKLEPLGLGARVVESVAAGPATPAAPKVHEEASSLRLLLAINA
                     VLFLVESVAGWLAQSTGLIADSLDMLADAFVYGLALWAVGRASAAKLRAAHASGVLQA
                     LLAAGVFFDVARRWVTGSAPEPPAMIGISALALAG"
     gene            642..1007
                     /locus_tag="P5164_17185"
     CDS             642..1007
                     /locus_tag="P5164_17185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45670.1"
                     /translation="MVEARPAPAALSFISSREEEPGRTARIVFLADAHAARPESAVVE
                     VTLLVGPEGTTVKAAARPLAEYGMRAPDAEGTASGCTPCQVTRTKLGFVPYSRGLATG
                     NSIFAVRCLAEALGGQPGK"
     gene            complement(1042..1731)
                     /locus_tag="P5164_17190"
     CDS             complement(1042..1731)
                     /locus_tag="P5164_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HRY45671.1"
                     /translation="MTPDLAPAAATALWLGVLTSISPCPLATNIAAVSYLARRADRPR
                     LVLLSALSYSLGRALAYAAVSAVVVLGLLSVPGVSQFLQRYMNKLLGPLLVLVGMYLL
                     GLISAGLSTTAGSEELRKKAAEGGAVGALFLGALFALSFCPVSAALFFGSLVPLSLQH
                     GSKVVLPVVYGFGTALPVAVLALVAAGGTKALGAALKKVGAFEVWARRLTGVVFVAVG
                     VYLSVRFVFLG"
     gene            complement(1728..2336)
                     /locus_tag="P5164_17195"
     CDS             complement(1728..2336)
                     /locus_tag="P5164_17195"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HRY45672.1"
                     /translation="MSIEKKHLVTGALLAFVAVTGVTFAVKETRHAQAVAAADAARPA
                     EPAPSAAIAEPPAPPAAAHVPTAPPAATPRPAPPAAKAPASRPAPEPSTVYVTYFHTT
                     ARCASCLKIEDLTNATMTTRFAGPIAEKRIVWRLVNVDEPVHAHFVKDYGLYTKSVVV
                     SEVKDGKEVRWKNLDQVWHLLGNPGSFQDYVEREVQAFLGTA"
     gene            complement(2333..3520)
                     /locus_tag="P5164_17200"
     CDS             complement(2333..3520)
                     /locus_tag="P5164_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRY45673.1"
                     /translation="MKERTKLLLIVGVFLGAYFLPIESARVQKALPEGFLLLRDYARE
                     HVLLCLVPAFFIAGAIGQFVSQGAVMKYLGSTAKRPVAYGVGSVSGAILAVCSCTVLP
                     LFSSIYRRGAGLGPATAFLYSGPAINVLAMILTARVLGLQLGLARAIGAVVFAIVIGL
                     LMAAIFRKEEAERVKNGPDPFAAAGAAASGRTREGWQDAAFLGSMIAILVFANWGASN
                     QAVGFFAAVYRVHWYLAGAALAALVFMMVRWYDRDELRLWVLGTWEFAKQILPLLLGG
                     VLVAGWLMGRPGHDSGLIPDAWVAGAVGGNSLAANLFASVAGAFMYFATLTEIPILQG
                     LLGSGMGKGPALALLLAGPALSLPSMIVINSYLGTKKTVVYVALVVVMSTITGLVFGA
                     FAS"
BASE COUNT          616 a         1219 c         1452 g          452 t
ORIGIN      
        1 cccggcgagg gcgagggcgg agatcccgat catcgcgggc ggctcgggcg cgctcccggt
       61 gacccagcgc cgcgcgacgt cgaagaacac acccgccgcc agaagcgcct ggaggacacc
      121 ggaggcgtgc gcggcccgca gcttcgcggc ggaggcccgg ccgacggccc agagggcgag
      181 gccgtagacg aaggcgtccg cgagcatgtc gagggagtcg gcgatgaggc cggtcgactg
      241 cgcgagccag ccggcgacgc tctcgacgag gaagaggacg gcgttgatgg cgaggaggag
      301 gcggagggag gaagcctcct cgtggacctt cggcgcagcg ggtgtcgcgg gaccagcggc
      361 gaccgactcg acgacgcgag ccccgaggcc gagaggttcg agcttcgcga ggagcgcacc
      421 gggctctccc cggtgggtca cggagagggt ccggctccgc aggtcgaacc tcaggtcgtc
      481 gacgccttcg actccgtcga gcgccatccg gacgagccgc tcctcggacg ggcagtccat
      541 cctcgagatg gcaaggaggg tcgtctgcag gccggcgggc gtcatcggac gggcatcgta
      601 gccgcttggc gggtcggatc ggcgaggccg gacccggacg catggtcgaa gcgaggcccg
      661 ccccggccgc tctctcgttc atcagctcgc gggaggagga gccgggacgg acggcacgta
      721 tcgtcttcct cgccgacgcg cacgcggccc ggccggagag cgccgtcgtc gaggtcacgc
      781 ttctcgtcgg accggagggg acgacggtaa aggccgccgc gcgccctctg gccgagtatg
      841 ggatgagggc gccggatgcg gagggcaccg cgtccggctg cacgccgtgt caggtcaccc
      901 ggacaaagct cggattcgtc ccgtacagcc gtgggttggc gacgggcaac tcgatcttcg
      961 ccgtccgctg cctcgctgag gccctgggcg ggcagcccgg gaagtgagcg gggcgcgggg
     1021 tcgtctcgac cgcgcggcgc ctcagcccag gaagacgaac ctcaccgaga ggtagacccc
     1081 gacggcgacg aagacgacgc cggtgaggcg ccgcgcccag acctcgaatg cccccacctt
     1141 cttcagcgcc gcgccgagcg ccttcgtccc tcccgcggcg acgagggcga ggacggcgac
     1201 cgggagcgcg gtgccgaagc cgtagacgac ggggaggacg accttcgagc cgtgctggag
     1261 cgacagcggg acgaggctgc cgaagaagag ggccgccgag accgggcaga acgagagggc
     1321 gaagagggct ccgaggaaga gcgcgccaac ggcgccccct tcggccgcct tctttcgcag
     1381 ctcctcgctc ccggccgtcg tcgagagtcc ggccgagatt aggccgagga ggtacatccc
     1441 gacgaggacg aggagaggcc ccaggagctt gttcatatag cgctggagaa actgcgagac
     1501 gccgggcacc gaaaggaggc cgaggacgac gacggcgctc acggcggcgt aggcgagggc
     1561 gcggccgagg gagtaggaga gggccgaaag gaggaccagg cgcgggcggt cggcgcggcg
     1621 cgcgagatac gagacggcgg cgatgttcgt cgcgagggga caggggctga tcgacgtcag
     1681 gacgccgagc cagagcgccg tcgcggcggc cggagcgagg tcgggcgtca agccgtgccc
     1741 aggaaggcct ggacctcccg ctcgacgtag tcctggaacg acccggggtt cccgaggagg
     1801 tgccagacct ggtcgaggtt cttccagcgg acctccttgc cgtccttcac ctcggagacg
     1861 acgacggact tcgtgtagag gccgtagtcc ttcacgaagt gggcgtggac gggctcgtcc
     1921 acgttcacga ggcgccagac gatccgcttc tcggcgatgg ggccggcgaa gcgcgtcgtc
     1981 atcgtggcgt tcgtgaggtc ctcgatcttc aggcacgagg cgcagcgcgc cgtggtgtgg
     2041 aagtaggtga cgtagaccgt ggacggctcg ggagcgggcc gcgaggcggg ggctttcgcg
     2101 gccggcggcg cggggcgggg cgtggccgcg ggaggcgcgg tcggaacgtg agcggcggcg
     2161 gggggcgccg ggggctctgc gatcgcggcc gaaggagcgg gctccgcggg ccgggcggcg
     2221 tccgctgcgg cgacggcctg cgcgtgccgc gtctccttca ccgcgaacgt gactcccgtg
     2281 acggcgacga aggcgaggag ggccccggtg acgaggtgct tcttctcgat gctcatgacg
     2341 cgaacgctcc gaagaccaga ccggtgatcg tggacatgac gacgacgagg gcgacgtaga
     2401 cgaccgtctt cttcgtcccg aggtacgagt tgatgacgat catgctcggc agggagaggg
     2461 ccgggccggc gaggaggagc gccagcgcgg gccctttgcc cattccggac ccgaggaggc
     2521 cctggaggat cgggatctcg gtgagcgtcg cgaagtacat gaaggcgccg gccacggagg
     2581 cgaagaggtt cgcggcgagg gagtttcccc cgaccgcccc tgcgacccac gcgtcgggga
     2641 tcagcccgga gtcgtgcccg gggcggccca tgagccaccc ggcgacgaga acgccgccga
     2701 ggaggagcgg gaggatctgc ttggcgaact cccaggtccc gaggacccag agccgcagct
     2761 cgtcccggtc gtaccagcgg accatcatga agacgagggc cgcgagggcc gcgccggcga
     2821 gataccagtg gacgcggtag accgcggcga agaagccgac ggcctggttc gaggcgcccc
     2881 agttcgcgaa gacgaggatc gcgatcatcg atccgaggaa ggcggcgtcc tgccacccct
     2941 cgcgcgtccg tccgctcgcc gcggcgccgg ccgccgcgaa cggatcgggc ccgttcttta
     3001 cccgctcggc ctcctccttc cggaagatcg cggccatgag cagcccgatg acgatggcga
     3061 agaccacggc cccgatcgcc cgggcgaggc ccagctggag gccgaggacc ctggcggtga
     3121 ggatcatcgc gaggacgttg atcgcgggtc cggagtagag gaacgccgtg gccgggccga
     3181 ggcccgctcc gcgcctgtag atgctcgaga agagtggaag aaccgtgcac gagcagacgg
     3241 cgaggatcgc gcccgagacg gagccgaccc cgtaggcgac cggccgcttt gccgtcgagc
     3301 cgaggtactt catcaccgcg ccctggctca cgaactgccc gatggcgccg gcgatgaaga
     3361 aggccggcac caggcagagc aggacgtgct cgcgggcgta gtcgcggaga aggaggaagc
     3421 cttccggaag cgccttctgg acccgcgcgc tctcgatcgg caggaagtac gcgccgagga
     3481 agaccccgac gatcaggagg agcttcgtcc gctccttcat cgcggggctc cggaggaccc
     3541 gggcgagctc cgccgggccg ccgccggcca gagcatcaga gggccgccag ggcggcccgg
     3601 agctcttcgg ccttcgggat ccggccggag aagatcacct tcccgtcgac ggcgatcccg
     3661 ggcgtggcga ggaggccgag ctcgatcatc cgctccattc cctcgacctt ctcgacggtg
     3721 aacgggagct tctcggtct
//
LOCUS       DAOTEZ010000427         3720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_18508_length_3720_cov_6.647203, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000427 DAOTEZ010000000
VERSION     DAOTEZ010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18508_length_3720_cov_6.647203"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="P5164_17205"
     CDS             complement(<1..303)
                     /locus_tag="P5164_17205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45674.1"
                     /translation="MSGDLPRVLFRTKRGLCPSCGVPLELDEDAPEVTCRFCGAPAVL
                     ERRLRRVEPEVEGAPFRLYLDAASAEAGGDPSATPWVRSKGFRQSVVARTLCPGCGE"
     gene            complement(320..1396)
                     /locus_tag="P5164_17210"
     CDS             complement(320..1396)
                     /locus_tag="P5164_17210"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRY45675.1"
                     /translation="MPDLADVLELVDATGDLLAARVPAEGPAVIRWGSQLVVREGQSA
                     LFLRDGRAMQLFEPGRHVLTTQNVAGLTGFVAGLAYGGETPFRAEVVFVGRQLFRDLR
                     WGTPEPVYIPDPVLLQVPVRANGRFAIRVADPALFVPKVVGTRPDFRQRDLEEFLRAQ
                     YVVSALTDALASLGKPFVELPRFTRELGTGVRAILAPEFAALGLELTDLSVNSVTTAE
                     EIQGTLNGNAKIASEAFAKAKGIQYDLQARAAGAEALKKAGTSYREVGATDALKELAA
                     NVGEGGGGSALDAGVSLGAAMLVPQMMQGMLRTEAAPGGPAAHAAEEVDPVARLKQLK
                     ELLDLGAITQEEYDAKKAEWLKRL"
     gene            complement(1396..1884)
                     /locus_tag="P5164_17215"
     CDS             complement(1396..1884)
                     /locus_tag="P5164_17215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45676.1"
                     /translation="MSGSARALLEKLGSLVPGYAGYADRETRRECDQALRLAVASRLG
                     AARAALDRRTADCARAGRLDLLEPLDSLSRRVAALADALRHAPAGYAALFDAATVDAA
                     QLDRIYAFDLEMKEAGERVFDVAERVRAGADAEALDPLEAAVAAAEESLRRRGEVLRE
                     VR"
     gene            2190..>3720
                     /locus_tag="P5164_17220"
     CDS             2190..>3720
                     /locus_tag="P5164_17220"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HRY45677.1"
                     /translation="MTGLVVLGAVILVLVATAVHDRLQKTHTIVSNFPVLGRFRYWFE
                     ALGGPLRQYIVTGNDEERPFSRDQRRWIYASAKKENSYFGFGTDNDLEKETGYVVVRH
                     STFPLHSPVPGEEGYDPGHPLPSAKVLGGARGRRHAFRPTSLVNTSAMSYGSLSHAAV
                     EAINRGVALAGAMQNTGEGGISDYHRKGGDIVWQIGTGYFGCRAENGRFSMERFLENV
                     ASARVRAVEVKLSQGAKPGLGGVLPAAKVTPEISRIRHVPVGKDCISPSAHSAFSDVS
                     SLLDFVERLADATGLPVGIKSAVGDLGFFEELARQIEATGRAPDFVTIDGGEGGTGAA
                     PLVFTDHVAMPFKLGFASVYRAFAERGVAERVVFVGSGRLGFPEQALLALALGCDMVN
                     VAREAMLSIGCIQAQVCHTGRCPTGVATQDPWLVGGLDPALKSVRLANYLVTLRKYLL
                     ALAHACGEVHPALVPLERIDILDGLSSRPAREVFGYQSGWGLPTEEERDAIRGLMKGG
                     SA"
BASE COUNT          494 a         1395 c         1334 g          497 t
ORIGIN      
        1 ctcgccgcag ccggggcaga gcgtccgcgc cacgacggac tgccggaagc ccttcgacct
       61 cacccagggc gtcgccgacg ggtccccccc cgcctcggcc gaggcggcgt cgaggtagag
      121 ccggaacggc gccccctcga cctccggctc gacccgccgg aggcgcctct cgagcacggc
      181 cggcgcgccg cagaagcggc acgtcacctc gggggcgtcc tcgtcgagct cgagggggac
      241 gccgcacgac gggcagaggc cgcgtttcgt ccggaagagg acgcgcggca ggtcgccgct
      301 caaggggcgc gtccgccgct cagagccgct tcagccactc ggccttcttc gcgtcgtact
      361 cctcctgcgt gatcgcgccg aggtcgagga gctccttgag ctgcttgagg cgcgcgacgg
      421 ggtcgacctc ttccgccgcg tgcgcggccg ggccgcccgg cgcggcttcc gtccgcagca
      481 tcccctgcat catctgggga acgagcatgg cggcgccgag cgagactccg gcgtcgagcg
      541 ccgagccacc gcccccctcg cccacgttcg ccgccagctc cttcagggcg tcggtcgctc
      601 ccacctcgcg gtagctcgtc ccggccttct tcagcgcctc cgccccggcg gcgcgcgcct
      661 ggaggtcgta ctggatcccc ttcgccttcg cgaaggcctc ggaggcgatc ttcgcgttcc
      721 cgttcagcgt cccctggatc tcctcggccg tcgtcaccga gttgacggag aggtccgtca
      781 gctcgaggcc gagcgcggcg aactcggggg cgaggatcgc gcgcacgccg gtgccgagct
      841 cccgcgtgaa ccgcggcagc tcgacgaacg gcttcccgag cgaggcgagg gcgtcggtga
      901 gggccgacac gacgtactgc gcgcgcagga actcctcgag gtcgcgctgg cggaagtccg
      961 gccgcgtgcc gacgaccttc gggacgaaga gggcggggtc ggcgacgcgg atcgcgaagc
     1021 gcccgttcgc gcgcaccggg acctgcagga ggaccgggtc ggggatgtag accggctccg
     1081 gcgtccccca gcgcaggtct cggaagagct gacgcccgac gaagacgacc tccgcgcgga
     1141 acggggtctc cccgccgtag gcgaggccgg cgacgaaccc ggtcagcccc gcgacgttct
     1201 gcgtcgtcag gacgtgccgc cccggctcga agagctgcat cgcgcgaccg tcgcggagga
     1261 agagcgcgct ctggccttcg cggacgacga gctgggagcc ccagcggatg acggcgggcc
     1321 cctcggccgg gacccgcgcg gcgaggaggt cgcccgtcgc gtcgacgagc tcgaggacgt
     1381 cggccaggtc gggcatcacc tcacctcccg gaggacttcg ccgcggcgac ggagggactc
     1441 ctccgcggcc gcgacggccg cttccagcgg atcgagagcc tcggcgtcgg caccggcccg
     1501 aacgcgctcc gccacgtcga acacccgctc gcccgcctcc ttcatctcga ggtcgaacgc
     1561 gtagatccgg tccagctgcg ccgcgtcgac cgtcgccgcg tcgaagagcg ccgcatagcc
     1621 cgcgggggcg tgtcgcaacg cgtcggccag agccgcgacc cggcgggaga gcgaatcgag
     1681 cggctcgagc aggtcgagcc ggcccgctcg ggcgcagtcg gccgtcctgc ggtcgagggc
     1741 ggcgcgggcc gcgccgaggc gcgaggcgac ggcgagccgc agcgcctggt cgcactcccg
     1801 gcgcgtctcc cggtccgcgt agcccgcgta gcccggcacg agggagccga gcttctcgag
     1861 gagcgctctc gccgagccgc tcatcggggt ctcctcgcgc cgaggaccgg gaccgcgccg
     1921 tcgcgttccc gcgccggccc cacgcccccg gggcgtcgtg cagacagccg ttgcctcatc
     1981 ttccggacct cctcgtgcgg gtcgatggcg ggagggtaat tcagagcgcc cctgttcggc
     2041 gaacggacgg cgcgccccgc cgccgaaggg tgtccgccgt cacccggacg ccgcggacct
     2101 tcctccgcga gcccgtcgcg gtgctagcct cgcccccttc cgtcaacccg gagggcgggc
     2161 gcgccgggcg gcgcgcgaag gggggaacca tgacgggact cgtcgttctc ggcgccgtga
     2221 ttctcgttct cgtcgcgacc gcggtccacg accgcctcca gaagacgcac acgatcgtct
     2281 cgaacttccc ggtcctcgga cgcttccgct actggttcga ggcgctcggc ggaccgctcc
     2341 ggcagtacat cgtcacgggg aacgacgagg agcggccctt cagccgcgac cagcgccgct
     2401 ggatctacgc gtcggcgaag aaggagaaca gctacttcgg gttcgggacc gacaacgacc
     2461 tcgagaagga gacgggctac gtcgtcgtcc gtcactccac gttcccgctc cactcgcccg
     2521 tcccgggcga ggaggggtac gaccccggcc atccgcttcc ctcggcgaag gtcctcggcg
     2581 gggcgcgcgg acgccgccac gccttccggc cgacgagcct cgtgaacacg tcggcgatga
     2641 gctacggctc cctgagccac gccgcggtcg aggcgatcaa ccggggcgtg gccctcgcgg
     2701 gcgcgatgca gaacaccggc gaaggaggca tctccgacta ccaccggaag ggaggcgaca
     2761 tcgtctggca gatcggcacc ggctacttcg gctgccgggc cgagaacggc cgcttctcga
     2821 tggagcgctt cctcgagaac gtcgcgagcg cgcgcgtgcg cgccgtcgag gtcaagctct
     2881 cgcagggggc caagccgggg ctcggcggcg tccttccggc cgcgaaggtg acgcccgaga
     2941 tctcgcggat ccggcacgtg cccgtcggga aggattgcat cagtccgtcg gcccattcgg
     3001 ccttctcgga cgtctcgtcc ctcctcgact tcgtcgagcg cctcgcggac gcgaccggcc
     3061 tccccgtcgg gatcaagagc gccgtcggcg acctgggctt cttcgaggag ctcgcccggc
     3121 agatcgaggc gaccgggcgc gcgcccgact tcgtgacgat cgacggaggc gagggaggga
     3181 ccggcgcggc gccgctcgtc ttcaccgacc acgtcgcgat gcccttcaag ctcggcttcg
     3241 cgagcgtcta ccgcgccttc gccgagcgcg gcgtcgccga gagggtcgtc ttcgtcggct
     3301 cggggaggct cggcttcccc gagcaggcgc tcctcgcgct cgcgctcggc tgcgacatgg
     3361 tgaacgtcgc ccgcgaggcg atgctctcga tcggctgcat ccaggcccag gtgtgccaca
     3421 cgggccgctg cccgacgggc gtcgcgacgc aggacccgtg gctcgtcggg gggctcgacc
     3481 cggcgctgaa gtccgtgcgc ctcgcgaact acctcgtgac gctccgcaag tacctcctcg
     3541 ccctggccca cgcgtgcggc gaggttcacc cggcgctcgt cccgctcgag aggatcgaca
     3601 tcctggacgg cctctcctcg aggcctgcgc gcgaggtgtt cggctaccag tccggctggg
     3661 gcctcccgac ggaggaggag cgggacgcga tccgcgggct catgaagggg ggctccgcga
//
LOCUS       DAOTEZ010000428         3696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_18669_length_3696_cov_6.033507, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000428 DAOTEZ010000000
VERSION     DAOTEZ010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18669_length_3696_cov_6.033507"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..3236
                     /locus_tag="P5164_17225"
     CDS             96..3236
                     /locus_tag="P5164_17225"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRY45678.1"
                     /translation="MRPNETSPRPEAGVDRREFLRLGSALAAAGALASAGCQPPQEAT
                     IPFHDMPESLAEGIGRARFFHTVVDGTPVLVRTREGRPILVAPSPTDASGRGLTLRHQ
                     AALMDLYDPDRARGPLSVRRSQGPTVASSWKTVGAEVASRLPKAGSGAVLLTGPVASP
                     ALSEAIAAISAGTGLRHVAWTPLGGGDAAAAAHRAAFGDERLPRPLLGKADLILGLGA
                     EFLDRPADGLERDFALRRSPDGPDAKMSRFVQLEGRLSLTGANADERVRVRDTQLASV
                     AAALAHELVVTRKTGPLAGNPEVAAALAPFAPEDVAKKAGLDPALLPRLAGELLAARG
                     KAIVLAGGAASGSAAGEALELAALLLNVTLDAYSAGLFDEAAAELPAAGGAAALAALV
                     EEMNAGKVSLLLVAGANPVYDAPPALRFAEALAKVPFVVSLNDRLDETSLLADLFAPA
                     SHTFECWGDAALPKGLLAIQQPVIQPLHDTRGLLDLLVEWGAAAGAGGELAAAVTAAT
                     AALAKLPLPASAAPAPSPSFAWHFLRAAWAPRLGAAPGSPELEKAWTELLRSGAFQGP
                     APAAPPARSLAPAALALLSSIPEAPEGLELQLYPHLALHDGRAGNNAWLQEFPDPITR
                     ISWGGALSIAPRRFDEMGLANGDLVEVDAGHGKLVAPAYRHAGMHADEVALPLGLGRA
                     SCGPIGAGVGPNAFALRLLSGARLLSAGLPVTLRKVKGHEELAFAQGSDVIDRDRRPL
                     VPVTTLTAYEANPKSGTEQQHGGPSAWPEHAYPNARWAMAIDLSKCNGCGKCVLGCQA
                     ENNIPVVGRRGIVDGREMSWMRIDRYYDAPKKEGRWGAEVWDGPLPVVEEPKVLFEPM
                     LCQHCENAPCETVCPFVATMHSEDGLNQQIYNRCVGTRYCANNCPFKVRRFNFWEYGK
                     AQESALFRALEPRIKRNAELNTRAPMQMKNNPEVTVRSRGVMEKCSFCVQRIREARAA
                     ATRDGKEKTHFPDGAVVPACMEACPTGAITFGDVNAPGSQVASLAAHPRAMRLLDALG
                     VKPSISYLTKVRNDKA"
     gene            3223..>3696
                     /locus_tag="P5164_17230"
     CDS             3223..>3696
                     /locus_tag="P5164_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014020318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45679.1"
                     /translation="MTRPEASPYATVMDDSQLTGRPIVLAGAGPYGGDLEVPEPLVTG
                     GKKWSDVDQDICMHAERFPTKRWWAAFAVALTLLGVLVVSLVTLLYAGMGIVGVNQPV
                     GWGSFIVNFVFWIGIGHAGTLISAVLYLFRQQWRTGINRAAEAMTLFAVACAGIFP"
BASE COUNT          484 a         1491 c         1241 g          480 t
ORIGIN      
        1 ccttcaacat gcagtggtgc ctcgactgcc accgcaagcc cgagatgaag gcctcgaccg
       61 actgcgtcgc ctgccaccgc tagagggaga tgacgatgag accgaacgag acctctcccc
      121 ggcccgaggc aggcgtcgac cggcgcgagt tcctccgcct cgggagcgcg ctcgcggcgg
      181 ccggcgccct cgcgagcgcg ggctgccagc cgccgcagga agcgacgatc ccgttccacg
      241 acatgcccga gagcctcgcc gaggggatcg ggcgggcccg cttcttccac accgtcgtcg
      301 acggcacgcc cgtcctcgtg aggacgcgcg aggggcgccc gatcctcgtc gccccgagcc
      361 cgaccgacgc gagcggccgg ggcctgacgc tccgccacca ggcggcgctg atggacctct
      421 acgaccccga ccgggcccgc ggcccgctct cggtccgcag gagccagggg ccgaccgtcg
      481 cctcgagctg gaagacggtc ggcgccgagg tcgcctcccg cctcccgaag gccggaagcg
      541 gcgccgtcct cctgacgggg cccgtcgcga gtcccgccct ctccgaggcg atcgccgcga
      601 tcagcgccgg cacgggcctc cgccacgtcg cctggacgcc cctcggcggc ggcgacgccg
      661 ccgcggcggc gcaccgcgcc gcgttcggcg acgagcgcct cccgcggccg ctcctcggga
      721 aggccgacct gatcctcggc ctgggcgccg agttcctcga ccgtccggcc gacggcctcg
      781 agcgcgactt cgcgctgcgg cgctcccccg acggccccga cgcgaagatg agccgcttcg
      841 tccagctcga aggacgcctc tcgctcaccg gcgcgaacgc cgacgagcgg gtgcgcgtcc
      901 gcgacacgca gctcgcctcc gtcgcggccg ccctcgccca cgagctcgtc gtgacccgga
      961 agacggggcc gctcgccggc aaccccgaag tcgccgccgc cctcgccccg ttcgcccccg
     1021 aggacgtcgc gaagaaggcg ggcctcgacc cggcgctcct tccccgcctc gccggcgagc
     1081 tcctcgccgc gcgcgggaaa gcgatcgtcc tcgcgggcgg cgcggcgagc ggctcggccg
     1141 cgggcgaggc gctcgagctc gccgcccttc tcctgaacgt gacgctcgac gcctactccg
     1201 ccggtctctt cgacgaggcg gccgccgagc tccccgccgc cggcggcgcc gccgccctcg
     1261 cggccctcgt ggaagagatg aacgccggga aggtctccct cctcctcgtc gccggcgcga
     1321 acccggtcta cgacgctccg cccgcgctcc gcttcgcgga ggcgctcgcg aaggtcccgt
     1381 tcgtcgtctc gctgaacgac cggctcgacg agacgagcct cctcgccgat ctcttcgccc
     1441 cggcgagcca tacgttcgag tgctggggcg acgcggcgct cccgaagggg ctcctcgcga
     1501 tccagcagcc ggtgatccag ccgctccacg acacgcgcgg cctcctcgac ctcctcgtcg
     1561 agtggggcgc cgccgccggg gcgggcggag agctcgccgc cgccgtcacc gccgccacgg
     1621 ccgcgctcgc gaagctcccg ctccccgcct ccgcagcccc cgccccgagc cccagcttcg
     1681 cctggcactt cctccgcgcc gcgtgggcgc cccggctcgg agctgctccc ggctcgcccg
     1741 agctcgagaa ggcctggacc gagctcctcc gctccggcgc cttccagggg cctgcccccg
     1801 cggcgcctcc cgcgcgttcg ctcgcccccg ccgccctcgc gcttctctcc tcgattccgg
     1861 aggcccccga gggcctcgag ctccagctct acccgcacct ggcgctccac gacgggcgcg
     1921 ccgggaacaa cgcctggctc caggagttcc ccgatccgat cacgcgcatc tcctggggcg
     1981 gggcgctctc gatcgccccg cgccgcttcg acgagatggg cctcgcgaac ggcgacctcg
     2041 tcgaggtcga cgcggggcac ggcaagctcg tcgcgcccgc ctaccgccac gccgggatgc
     2101 acgccgacga ggtggccctc ccgctcggcc tcggccgcgc ctcctgcggc ccgatcggtg
     2161 ccggcgtcgg cccgaacgcc ttcgccctcc gcctcctctc gggcgcccgc ctcctctcgg
     2221 ccggcctccc ggtcacgctc cggaaggtga agggtcacga ggagctcgcg ttcgcgcagg
     2281 ggtccgacgt catcgaccgc gaccgccggc cgctcgtccc ggtcacgacg ctcacggcgt
     2341 acgaggcgaa tccgaagtcg gggaccgagc agcagcacgg cggcccttcc gcctggcccg
     2401 agcacgccta cccgaacgcc cgctgggcga tggcgatcga cctttcgaag tgcaacggct
     2461 gcggcaagtg cgtcctcggc tgccaggccg agaacaacat cccggtcgtc gggcgccgcg
     2521 ggatcgtcga cgggcgcgag atgtcgtgga tgcggatcga ccgctactac gacgccccga
     2581 agaaggaggg ccgctggggc gcggaggtct gggacggccc gctgccggtc gtcgaggagc
     2641 cgaaggtcct cttcgagccg atgctctgcc agcactgcga gaacgcgccg tgcgagaccg
     2701 tctgcccgtt cgtcgccacg atgcacagcg aggacggcct gaaccagcag atctacaacc
     2761 gctgcgtcgg gacccgctac tgcgcgaaca actgcccgtt caaggtgcgg cgcttcaact
     2821 tctgggagta cggcaaggcg caggagagcg ccctcttccg cgccctcgag ccccgcatca
     2881 agcggaacgc ggagctgaac acccgcgccc cgatgcagat gaagaacaac cccgaggtga
     2941 ccgtgcggag ccgcggcgtg atggagaagt gctccttctg cgtccagcgg atccgcgagg
     3001 cgcgggccgc ggcgacgcgc gacgggaagg agaagacgca cttccccgac ggcgccgtcg
     3061 tgccggcctg catggaggcc tgcccgaccg gcgcgatcac gttcggcgac gtgaacgccc
     3121 cgggatcgca ggtcgcctcg ctcgccgccc acccgcgggc gatgcgcctc ctcgacgcgc
     3181 tgggcgtcaa gccctccatc tcctacctga cgaaggtgcg caatgacaag gcctgaggcg
     3241 agcccctacg ccacggtgat ggacgacagc cagctcacgg gccggccgat cgtcctcgcg
     3301 ggagccggcc cctacggcgg cgacctcgag gtccccgagc cgctcgtcac cggcgggaag
     3361 aagtggtccg acgtcgacca ggacatctgc atgcacgccg agcgcttccc cacgaagagg
     3421 tggtgggccg cgttcgccgt ggcgctgacg ctcctcggcg tcctcgtcgt ctcgctcgtc
     3481 accctcctct acgccggaat ggggatcgtc ggggtcaacc agccggtcgg ctggggctcc
     3541 ttcatcgtca acttcgtctt ctggatcggc atcggccacg ccgggacgct catctcggcg
     3601 gtcctctacc tcttccgcca gcagtggcgg acggggatca accgggccgc cgaggcgatg
     3661 acgctcttcg ccgtcgcctg cgccggcatc ttcccg
//
LOCUS       DAOTEZ010000429         3687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_18749_length_3687_cov_6.534967, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000429 DAOTEZ010000000
VERSION     DAOTEZ010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18749_length_3687_cov_6.534967"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2509)
                     /locus_tag="P5164_17235"
     CDS             complement(<1..2509)
                     /locus_tag="P5164_17235"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRY45680.1"
                     /translation="MIRTRPALLLAGALLATPPAAASLRGKVLLDGRPVAGATVSAIP
                     QEESLDAARREARREPEPAALASVATSATGEFDLRVAAPEGRNVLFRLRVSAAGALPV
                     ELPETRDAAEAEDLGDLVLRGAEPLAGRVRGPDGAPVAGALVRLRAFARTDGTAAGTA
                     RTQETTTGADGQFRFDAAAAEGNELFVQAAGFAPARVRSAKPGALPRAISLEPGLSLP
                     GVVRRKDGRPAAKALVRYEADDAESRWVETGEDGSFRLADLPKRRGTVVADGGEDGFA
                     EAGGAMPGSAAPLVLSLAPPAILEGRTLDATTLKPVPRVRLEVASGSGRFTARSGSDG
                     RYRLRGLRPGSATVRAEEPRHVPWTRRPVPIEKGATRALDVLLTRGATLSGRVVDEDG
                     RPVAGAGVSLLSSSESPIDAAVRLRSGAASRLRTRPDGSFTATRLPPGENQRLSVQHP
                     EFERGTVGGISLVAGGTRSGVVVTIRRGVVLTGRVSDPDGNPIAGAEVSAGRSFSVRS
                     SSGGSLTRVRAEMMEAPRTVRSGGDGAFELRGLAPGDVALVVRAPGRATETVDPVRLV
                     RGGRPDPVEIVLGPGATISGRIVRRTGEGASGYYVLARNPRKPSDGPGNTSATPEADG
                     TFRIEGLRAGETYDLQLFGGGAGFGPGPRKAGILAPADGVEWVVEGTGRIEGSAVDGK
                     TGQPLGAFEVSYQADGPGMGGVFRVGRGGGRFTGGAGEAQLVESPDGRFALEEVPAGK
                     WQVVVTAKGYQVGRAAGIVVEEATTTEGVEVRVAPGSVLRGVVKDARTRRGIPDARVR
                     VRGETGDAGPSQGSGGLFTDAEGRFEAEGLAPGKVTVT"
     gene            complement(2633..>3687)
                     /locus_tag="P5164_17240"
     CDS             complement(2633..>3687)
                     /locus_tag="P5164_17240"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRY45681.1"
                     /translation="DAALAEHGLLFPIDLGADPSIGGMVATNTGGTRLLRYGDIRQNL
                     LGLEAVLADGTVLDLTRPLRKNNTGLDARQLFVGTSGVFGVVTKVVMRVVPRPTQRAT
                     ALVGLSSGSAVLGLLGHLESRLGELLTAFEAIGAAALAPVFRHQPRLRDPFAGRPPAY
                     AVLVELSSTLGAERLALDDLLESALAERLEAADGGITDVFPGKPSELWEIRHGVSESL
                     RQEGETLAFDVSVPRSSLAAFVDAARETVAAGWPFVRFCDYGHWGDGGVHVNLIWNPA
                     EAPRPAADLRAELQPRLYDLAVVRFGGSYSAEHGVGPHNQAFYDLYTPELVKEVCRLL
                     KQRLDPAGLLGTVRLG"
BASE COUNT          470 a         1408 c         1396 g          413 t
ORIGIN      
        1 ccgtgaccgt caccttcccc ggggcgaggc cttccgcctc gaaccggccc tccgcgtcgg
       61 tgaagaggcc gcctgatccc tgcgacggcc cggcgtcccc cgtctccccg cggacgcgaa
      121 cccgcgcgtc cggaatgccg cggcgggtgc gcgcgtcctt cacgacgccc cggaggacgc
      181 tccccggcgc cacgcggacc tcgacgccct cggtcgtcgt cgcctcctcg acgacgatgc
      241 cggcggcccg gccgacctgg tagcccttcg ccgtcacgac gacctgccac ttcccggcgg
      301 gcacctcctc gagcgcgaag cgcccgtccg gcgattcgac gagctgcgcc tcgccggcgc
      361 cgcccgtgaa ccggcccccg ccccggccga cgcggaagac gccccccatc cccggcccgt
      421 cggcctggta cgagacctcg aacgcgccga gcggctgccc ggtcttcccg tcgacggccg
      481 agccttcgat ccggccggtc ccctcgacga cccactcgac gccgtcggca ggcgcgagga
      541 ttccggcctt gcggggcccc ggaccgaacc cggcgccgcc cccgaagagc tggaggtcgt
      601 acgtctcgcc ggcgcgaagc ccctcgatcc ggaacgtgcc gtcggcctcc ggcgtcgccg
      661 acgtgttccc cgggccgtcc gagggctttc gcgggttccg cgcgaggacg tagtagcccg
      721 aggcgccttc ccccgtccgg cggacgatcc ggccggagat cgtcgccccc gggccgagga
      781 cgatctccac cgggtccggc cggccgccgc gcacgaggcg gaccgggtcg acggtctcgg
      841 tcgcccgccc cggcgccctc acgacgaggg cgacgtcgcc cggagcgagg ccgcgcagct
      901 cgaaggcccc gtcgcctccg ctccggaccg tgcgcggcgc ctccatcatc tcggcccgca
      961 ctcgcgtcag cgatccgccg ctggaggaac gaacggagaa cgaccggccc gccgagacct
     1021 cggctccggc gatcgggttc ccgtccgggt ccgagacccg gcccgtgagg acgacgcctc
     1081 ggcgaatcgt gacgacgacg ccgctgcgcg tccctccggc gacgagggag atcccgccaa
     1141 cggtccccct ctcgaactcg ggatgctgga cactcaggcg ctggttctcg ccgggcggga
     1201 ggcgggtcgc cgtgaaggag ccgtcgggcc gcgtccggag gcggctcgcg gctccgctcc
     1261 ggagccggac cgcggcgtcg atcgggctct cggacgagga gaggagcgag acgccggcac
     1321 cggccaccgg tcggccgtcc tcgtcgacga cccgccccga gagcgtcgcg ccgcgcgtca
     1381 ggaggacgtc gagcgcgcgg gtcgccccct tctcgatcgg caccggacgg cgcgtccagg
     1441 ggacgtgccg cggctcctcg gcccgcacgg tcgcgcttcc ggggcgaagg ccgcgaagcc
     1501 ggtagcggcc gtcggacccc gagcgcgccg tgaaacggcc ggagcccgag gcgacctcga
     1561 gccgaacgcg cgggaccggc ttcagggtcg tcgcgtcgag ggtccgcccc tcgaggatcg
     1621 ccgggggcgc gagggaaagg acgagcggcg ccgcggagcc cggcatcgcc ccgcccgcct
     1681 cggcgaagcc gtcctcgcct ccgtccgcga cgacggtgcc gcggcgcttc gggaggtcgg
     1741 cgaggcggaa gctcccgtcc tcccccgtct ccacccagcg agactcggcg tcgtcggcct
     1801 cgtagcggac gagggccttc gccgcggggc gtccgtcctt cctccgcacg acgcccggga
     1861 gcgagaggcc cggctcgagc gagatcgcgc gcggcaacgc gcccggcttc gcgctgcgga
     1921 cgcgggccgg cgcgaagccg gccgcctgca cgaagagctc gttcccttcc gcggcggccg
     1981 cgtcgaaccg gaactgcccg tcggcccccg tcgtcgtctc ctgggtccgc gccgtcccgg
     2041 ccgcggtgcc gtccgtccgg gcgaacgcgc ggagacgcac gagagccccg gcgacggggg
     2101 ctccgtccgg tccgcggaca cggcccgcga gcggctccgc gccccggagg acgaggtcgc
     2161 cgagatcctc cgcctccgcc gcgtcgcgcg tctccggcag ctccaccgga agggcgccgg
     2221 ccgccgacac ccggagacgg aagaggacgt tgcggccctc gggcgcggcg acccgcaggt
     2281 cgaactcccc cgtcgccgag gtggcgaccg acgcgagcgc cgcaggctcc ggctctcggc
     2341 gcgcctcgcg gcgcgccgcg tcgagcgact cctcctgcgg gatcgcggag accgtcgcgc
     2401 cggcgacagg ccggccgtcg aggaggacct ttccgcgaag ggaagcggcg gcgggcggcg
     2461 tcgcgagcag ggcgcctgcg aggaggagag cgggtcgagt gcggatcaag cggcctccgt
     2521 gcgttcaccg gagcggccgg gcgcggcccc ggggagcggg tgcgtgttac cgacagtgta
     2581 cggattcgga cggggggtcg tcgcgccacg acgggtggcg acgaatccgc cctcagccca
     2641 gccggaccgt cccgaggaga cccgcggggt cgaggcgctg cttgagcaga cgacagactt
     2701 ccttcacgag ctccggcgtg tagaggtcgt agaaggcctg gttgtgcggg ccgacgccgt
     2761 gctcggcgct gtagctgccg ccgaagcgga cgacggcgag gtcgtagagc cgcggctgca
     2821 gctcggccct caggtcggcc gccggccgcg gcgcctccgc cgggttccag atcaggttca
     2881 cgtggacgcc gccgtcgccc cagtgaccgt agtcgcagaa gcggacgaac ggccagccgg
     2941 cggcgaccgt ctcgcgcgcg gcgtcgacga aggcggcgag cgaggagcgc gggacggaga
     3001 cgtcgaaggc gagcgtctcg ccctcctgcc ggaggctctc ggagacgccg tggcggatct
     3061 cccagagctc cgacggcttc ccgggaaaga cgtccgtgat cccgccgtcc gcggcctcga
     3121 ggcgctcggc gagcgccgac tcgagaaggt cgtcgagcgc gagccgctcc gcgccgagcg
     3181 tcgaggagag ctccacgagg accgcgtagg ccgggggtcg gccggcgaac gggtcgcgca
     3241 ggcgcggctg gtgccggaag acgggcgcga gcgcggcggc gccgatcgcc tcgaaggcgg
     3301 tgagcagctc gccgaggcgc gactcgagat gcccgaggag gccgaggacg gcggaacccg
     3361 aggagaggcc gacgagcgcc gtcgcccgct gggtcggccg cgggacgacg cgcatcacga
     3421 ccttcgtcac gactccgaag accccggacg tgccgacgaa gagctggcgc gcgtccagcc
     3481 ccgtgttgtt cttccggagc gggcgcgtca ggtcgaggac ggtcccgtcg gcgaggaccg
     3541 cctcgaggcc gaggaggttc tgccggatgt cgccatagcg caggagacgc gtgccgccgg
     3601 tgttcgtcgc gaccatgccg ccgatcgagg ggtcggcccc gaggtcgatc gggaagagga
     3661 ggccgtgctc ggcgagcgcg gcgtcga
//
LOCUS       DAOTEZ010000430         3682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_18781_length_3682_cov_5.674938, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000430 DAOTEZ010000000
VERSION     DAOTEZ010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18781_length_3682_cov_5.674938"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..85)
                     /locus_tag="P5164_17245"
     tRNA            complement(1..85)
                     /locus_tag="P5164_17245"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50..52),aa:Leu,seq:taa)
     gene            complement(178..253)
                     /locus_tag="P5164_17250"
     tRNA            complement(178..253)
                     /locus_tag="P5164_17250"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(218..220),aa:Cys,seq:gca)
     gene            complement(309..1490)
                     /locus_tag="P5164_17255"
     CDS             complement(309..1490)
                     /locus_tag="P5164_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRY45682.1"
                     /translation="MPTADAAAPVVPPLSILTESEREIAENVHQFAVETIGPRSLAMD
                     LANRMDPELVRQFFDLDLMGIEIPEEYGGLGQNFSTAIAVIEALARVDAACAVCVDVQ
                     NTLVDNAVLRWGSPGLKAKYLPQLAKEKVGSYALSEAESGSDAFALKTRAEKKGDRWV
                     LNGGKLWITNGLEAEIFLVFANVDFSKGYKGITAFVVESGWKGFTKGKKEDKLGIRAS
                     STLALAFEDLEVPEENVLGKVGEGYKIAIETLNEGRIGIGAQMLGVARAALDYSAKYV
                     QTRVQFGKPLSEFQAVQHELGRMDMELECARLAVYNAARLKEAGKPFAREGAIAKLKA
                     SEVAEYCTSKGLELLGGYGYVREYPLEKMYRDAKIGKIYEGTSYMQLSTIAKDVLKRN
                     A"
     gene            1833..>3682
                     /locus_tag="P5164_17260"
     CDS             1833..>3682
                     /locus_tag="P5164_17260"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRY45683.1"
                     /translation="MARLSRSRVRWASTVLTVVLALGTATVAMAQETTARLFGAVTMK
                     SDQSPLPGVSVEAIHVPTGTRYTALTQANGRYTILNARVGGPYTITATISGFRPSTQK
                     DISLALGEGRQVNFGLDLESVTETVVVTAEGTPLITPDRMGSGSALPEETLKALPTIN
                     RQFQDMARTNPYVNVAAYDESGTNISVSGKNNRYNTIQIDGAVNNDLFGLAATGTPGG
                     QSNTQPISMDSIQELQVVVSPYDVKQGGFTGGAINAITRGGTNTLSASVYGSTRNEDF
                     IGSKVPRATGPASDRPYAMFDYDQYGARIGGPILKDKLFFFVSGEKNKRSEPTSGSAD
                     GSTSNAYKDPAGAARFRNILINKYGYDPGGLGDYNGATESNLLFGRLDFNVNESHNAT
                     LRHNYVDAVRDATPARNFNVYGFETAGYGFADETNSTVLQVNSVFGSSSFNTGRIGYQ
                     TIVDSRDVPVLFPSVYICNSPNSFCTGNNTYTLLAGTERSSGANGLDQKILEVTDDFT
                     LIKGNHTITIGTHNEFFDFENLFIQDITGTYFFRSLDALENGIADRYQVKFANGADPR
                     RPTAFGAQQWGIYAGDQWRVNDKLTLNLGLRFDVPRLTDKPTYNAAVESA"
BASE COUNT          625 a         1330 c         1138 g          589 t
ORIGIN      
        1 tgccggagga gggactcgaa ccctcacggc ccttcggggc ccggggcttt taaggcccct
       61 gcgtctgcca ttccgccact ccggcccagt cgtcccggac ggtcgatcgt agaccggggc
      121 gcgcccggcc tcgaaaagag aaccgccgcg cccggagacg cggcggcggt ttcattctgg
      181 aggcgacggt cggattcgaa ccgacgaatg aggcttttgc agagccttcc cttaaccact
      241 tgggtacgtc gccctgggag gcggattcta gcagcgggcg gtccggcggg ccagacgcct
      301 tcccggggtc aggcgttccg cttcaggacg tccttcgcga tcgtcgagag ctgcatgtac
      361 gacgtcccct cgtagatctt cccgatcttc gcgtcgcggt acatcttctc gagcgggtac
      421 tccctcacgt agccgtagcc gccgaggagc tcgagcccct tcgacgtgca gtactccgcg
      481 acctccgagg ccttcagctt cgcgatcgcc ccctcgcgcg cgaacggctt ccccgcctcc
      541 ttcaggcggg cggcgttgta gacggcgagg cgggcgcact cgagctccat gtccatccgg
      601 ccgagctcgt gctggaccgc ctggaactcc gacagcggct tgccgaactg gacgcgggtc
      661 tggacgtact tcgccgagta gtcgagggcc gcacgggcga cgccgagcat ctgcgcgccg
      721 atcccgatcc gcccctcgtt cagcgtttcg atggcgatct tgtacccctc gccgaccttc
      781 ccgaggacgt tctcctccgg gacctccagg tcctcgaacg cgagcgcgag ggtggaggag
      841 gcgcggatgc cgagcttgtc ctccttcttc cccttcgtga agcccttcca gcccgactcg
      901 acgacgaagg cggtgatccc cttgtacccc ttcgagaagt cgacgttcgc gaagacgagg
      961 aagatctcgg cctcgaggcc gttcgtgatc cagagcttgc cgccgttcag gacccagcgg
     1021 tcccccttct tctcggcgcg cgtcttcagc gcgaaggcgt cggagcccga ctcggcctcc
     1081 gagagggcgt aggagccgac cttctccttc gcgagctgcg gcaggtactt cgccttcagc
     1141 ccgggcgagc cccagcggag gaccgcgttg tcgacgagcg tgttctggac gtcgacgcag
     1201 acggcgcagg cggcgtcgac gcgcgcgagc gcctcgatca ccgcgatcgc ggtcgagaag
     1261 ttctgcccga ggccgccgta ctcctccggg atctcgatcc ccatcaggtc gaggtcgaag
     1321 aactggcgga cgagctccgg gtccatccgg ttcgcgaggt ccatggcgag agagcgcggg
     1381 ccgatcgtct cgacggcgaa ctggtggacg ttctcggcga tctcccgctc ggactccgtc
     1441 aggatcgaca ggggcgggac gacgggggct gccgcatcgg ctgtgggcat cggtcactcc
     1501 ttgcgggacg aggatagaac gaatcgccgg agcgccggcc ccgagcggtc gtcgggcgaa
     1561 gggagcgacc ggcgagccgc ccgccggcca ccaccgagcc cggcggaaag tgcggagatg
     1621 cgcgctccga cctctttccg tcgttgacgc tccccttccc gctcctatag actcctccgc
     1681 ggcttgcgga gggctccacg gagccgctct gggggtgcct gattttcctc gctcgccgtc
     1741 tcaaagcttt ttcatcgaat tcacaatcat cgggtgtgct tcctgacagg atgtacgtgt
     1801 cctcaatcga tttgaggagg aggaaaggcc atatggcaag actcagtcgt tcccgggtcc
     1861 gttgggcgtc gaccgtcctg acggtcgtcc tcgccctggg caccgccacg gtggcgatgg
     1921 cccaggagac cacggcccgc ctcttcggcg cggtcacgat gaagtccgac cagagcccgc
     1981 tcccgggcgt ctcggtcgag gcgatccacg ttccgacggg gacgcgctac acggcgctca
     2041 cccaggcgaa cggccggtac acgatcctga acgcccgcgt cggcggaccg tacacgatca
     2101 ccgcgaccat cagcggcttc cgcccgtcga cccagaagga catctccctc gccctcggcg
     2161 agggccgtca ggtgaacttc gggctcgacc tcgagtccgt caccgagacg gtcgtcgtca
     2221 cggccgaggg gacgcccctc atcactcccg accgcatggg ctccggctcg gcgctccccg
     2281 aggagacgct caaggcgctg cccacgatca accggcagtt ccaggacatg gcgcggacga
     2341 acccgtacgt caacgtcgcg gcctacgacg agtcgggaac gaacatctcg gtctcgggca
     2401 agaacaaccg gtacaacacg atccagatcg acggcgcggt caacaacgac ctcttcggtc
     2461 tcgccgccac cggcacgccg ggcggccagt cgaacacgca gccgatctcg atggactcca
     2521 tccaggagct ccaggtcgtc gtctccccct acgacgtcaa gcagggcggc ttcacgggcg
     2581 gcgcgatcaa cgccatcacc cgcggcggga cgaacacgct ctccgcctcc gtctacggct
     2641 cgacgcgtaa cgaggacttc atcggcagca aggtcccgcg cgccaccggc cccgcgtccg
     2701 accggccgta cgcgatgttc gactacgacc agtacggcgc ccgcatcggc ggcccgatcc
     2761 tgaaggacaa gctcttcttc ttcgtcagcg gcgagaagaa caagcgctcc gagccgacga
     2821 gcggctccgc cgacggctcc acgtcgaacg cctacaagga cccggcgggc gccgcccgct
     2881 tccggaacat cctgatcaac aagtacgggt acgacccggg cggactcggc gactacaacg
     2941 gcgcgacgga gagcaacctg ctcttcggac ggctcgactt caacgtcaac gagagccaca
     3001 acgcgacgct ccggcacaac tacgtcgacg ccgttcgcga cgcgacgccg gcccggaact
     3061 tcaacgtcta cggcttcgag accgcgggct acgggttcgc cgacgagacg aactccaccg
     3121 tcctgcaggt caacagcgtc ttcggctcga gctccttcaa cacgggccgc atcggctacc
     3181 agacgatcgt cgacagccgc gacgtcccgg tgctcttccc ctcggtctac atctgcaact
     3241 cgccgaactc gttctgcacc ggcaacaaca cctacacgct cctcgccggc accgagcgct
     3301 cctccggggc gaacggcctc gaccagaaga tcctcgaggt caccgacgac ttcacgctga
     3361 tcaagggcaa ccacacgatc acgatcggga ctcacaacga gttcttcgac ttcgagaacc
     3421 tcttcatcca ggacatcacc ggcacgtact tcttccgcag cctcgacgct ctcgagaacg
     3481 gaatcgccga ccgctaccag gtgaagttcg cgaacggcgc cgacccgcgc cggccgaccg
     3541 cgttcggtgc ccagcagtgg ggcatctacg cgggcgacca gtggcgcgtc aacgacaagc
     3601 tgaccctgaa cctcggcctc cgcttcgacg tcccgcgcct gaccgacaag ccgacctaca
     3661 acgcggccgt cgagtcggcc tt
//
LOCUS       DAOTEZ010000431         3665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_18900_length_3665_cov_6.205540, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000431 DAOTEZ010000000
VERSION     DAOTEZ010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18900_length_3665_cov_6.205540"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="P5164_17265"
     CDS             <1..810
                     /locus_tag="P5164_17265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45684.1"
                     /translation="PKAFAMGVRIEHPQDDVDRIQYGRLASSCGVMPADYKMTAQTSV
                     GRAVYTFCMCPGGEVIACSSEAGGVVTNGMSRYKRASGFANSGLVVQTQPGDFAGEGG
                     PEVLRGIAFQRRVERAAFAAGGGTYAAPAVRVTDFLAGRTPRALPRGTYAPACVPARL
                     AGVYPDGYLVALAEALRFFDRKMRGFVSEEAVLIAPESRTSSPVRIERNEACESPSLP
                     GLFPAGEGAGFAGGIVSAALDGLRVARAVRARLAGEVPPPSSARPVEAPEY"
     gene            complement(815..1609)
                     /locus_tag="P5164_17270"
     CDS             complement(815..1609)
                     /locus_tag="P5164_17270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45685.1"
                     /translation="MKTTTTRRATAEELAKLEGYLNPKPKRNPAWAATVMVTVPLAWA
                     LFVVLNMLLDGWVPAAVRLLLAVAIAGTGAFLFGKRLKAQHEASARPSEPVLGFLQRD
                     LESGRVVVTRWEVDAVVKVASDPSRFVSTTWFAKLTDGTVALLVPPDLEEAELSGDFP
                     AASFEIATGEESRHVLSIRRTGERLEPAALRGPLSDGEWEELGEEADAPVPFGWEEVL
                     ARAAANPLRTRAKGEARSRQEALEREILKAREEEAGRATPDPTRPT"
     gene            1690..3597
                     /gene="selB"
                     /locus_tag="P5164_17275"
     CDS             1690..3597
                     /gene="selB"
                     /locus_tag="P5164_17275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HRY45686.1"
                     /translation="MARVLVGTAGHIDHGKSALVRALTGTDPDRLPEEKARGITIDLG
                     FAHAAWGGTTFSFVDVPGHEKLVRTMVAGAAGIDVALLVVASDDSVMPQTREHLAILS
                     LLDVRCGVVARTKADLVDEETGAVVEAEIADLVSGTFLEGAPVVAVSSLTGRGLDALR
                     EALLREASASDARDPAARPARLYVDRVFTMKGFGPVVTGTLDSGRVRVEDRLALLPEG
                     REVRVRRLEVHGEERDSAEAGERTSLNLAGVETADLGRGRALVAPESLEAATLLTVEV
                     TALPDLGAPIEDGLAVRLHTGTADVAARLHLLGGGDGAPTLAPGERAVAQVSALEPVA
                     ARRGDRFVLRRPSPQETIGGGRVLDVGRPRVRRRAPLSAHAASVLAGDDERAVAALLL
                     SEAGPAGIDVGMLARRLGVPAATAAAHLDALVASGAALRLTPALAVSASASVEMATRA
                     DAFFAERRKAGVPTLLVGRRELLGKIARGLPEATAEAWLATLAAARRLVVTADQAGPP
                     GAAASAVADEAGAFASRIEEAWRTAGFDAPGNLDLAKALGTKPQVVAGLVQHLLKSGA
                     LVRLSPEVFVHAELLGGIEAKVAEQKGRTMSVGDFRDLLGLSRKYLIPLLEHLDRKKV
                     TRRVGDARVVV"
BASE COUNT          436 a         1406 c         1337 g          486 t
ORIGIN      
        1 ccgaaggcgt tcgcgatggg ggtgcggatc gagcacccgc aggacgacgt cgaccgaatc
       61 cagtacgggc ggctcgcctc ctcctgcggc gtcatgccgg ccgactacaa gatgacggcc
      121 cagacctccg tcgggcgtgc cgtctacacg ttctgcatgt gccccggcgg cgaggtgatc
      181 gcctgctcgt cggaggcggg gggcgtcgtg acgaacggga tgtcccgcta caagcgcgcc
      241 tccggcttcg cgaactcggg cctcgtcgtc cagacgcagc ccggcgactt cgcgggcgag
      301 ggcggacccg aggtcctccg cggcatcgcg ttccagcgtc gggtcgagcg ggccgctttc
      361 gcggcgggag gcgggacgta cgccgccccg gctgtccgcg tgacggactt cctcgccggc
      421 cgcacgcccc gcgccctccc gcgcggcacc tacgcgcccg cctgcgtccc ggcccgcctc
      481 gcgggggtct atcccgacgg ctacctcgtc gcgctcgccg aggcgctccg cttcttcgac
      541 cggaagatgc gcggcttcgt ctcggaagag gcggtcctga tcgcgccgga gtcgcggacc
      601 tcctcccccg tgaggatcga gcggaacgag gcgtgcgagt cgccctcgct ccccggcctc
      661 ttccccgcgg gcgagggggc cggcttcgcc ggagggatcg tctcggcggc gctcgacggg
      721 ctgcgcgtgg cccgcgcggt acgggcgcgg ctggccggcg aggtgccgcc gccgtcctcg
      781 gcccggcccg tcgaagcgcc cgagtactga agcgctacgt cggacgggtc gggtcgggcg
      841 tcgcccttcc cgcctcttcc tcgcgcgcct tcaggatctc gcgctcgagc gcctcctgcc
      901 gggagcgggc ctcgcccttc gcccgcgtgc ggagcgggtt cgccgccgcg cgcgcgagaa
      961 cctcctccca gccgaagggg accggggcgt cggcctcctc gccgagctcc tcccactcgc
     1021 cgtcggaaag cggcccgcga agcgccgccg gctcgagcct ctcaccggtc cgccggatgg
     1081 agaggacgtg ccggctctcc tcgccggtcg cgatctcgaa ggaggccgcc gggaagtcgc
     1141 cggagagctc cgcctcctcg aggtcgggcg ggacgagaag ggccacggtt ccgtccgtca
     1201 gcttcgcgaa ccaggtcgtc gagacgaagc ggctcggatc ggaggcgacc ttcacgaccg
     1261 catcgacctc ccagcgcgtg acgacgacgc gccccgattc gaggtcccgc tgcaggaacc
     1321 ccaggaccgg ctcggaggga cgggccgatg cctcgtgctg cgccttcagc cgcttcccga
     1381 agagaaaggc cccggtgccg gcgatcgcga cggcgaggag gagccgaacg gcggccggga
     1441 cccaaccgtc gagcagcatg ttcaggacga cgaagagggc ccacgcgagc ggcacggtga
     1501 ccatcacggt cgcggcccac gccggattcc tcttcggctt cgggttcagg tacccctcga
     1561 gcttcgcgag ctcctccgcc gtggcccgtc gcgtcgtcgt cgtcttcatg aacgccgaat
     1621 gatgccaccc gcccctgggc gccgccccgc cacggtgacg ctctcccggc gatccctgat
     1681 actcctccca tggcgcgcgt cctcgtcggc accgcgggac acatcgacca cggcaagagc
     1741 gcgctcgtcc gggcgctgac cgggacggac ccggaccgcc tccccgagga gaaggcgcgc
     1801 gggatcacga tcgacctcgg cttcgcgcac gcggcgtggg gcggcacgac gttctcgttc
     1861 gtcgacgtcc cggggcacga gaagctcgtc cggacgatgg tcgccggcgc cgccgggatc
     1921 gacgtcgcgc tcctcgtcgt cgcctccgac gactcggtca tgccccagac gcgcgagcac
     1981 ctcgcgatcc tctcgctcct cgacgtgcgc tgcggcgtcg tcgcgaggac gaaggccgat
     2041 ctcgtggacg aggagacggg agcggtcgtc gaggccgaga tcgccgacct cgtctccggg
     2101 acgttcctcg agggggctcc tgtcgtggcc gtctcttcgc tgaccgggcg cggcctcgac
     2161 gcgctgaggg aggccctcct ccgggaagcc tcggcctccg acgcgcgcga cccggccgcg
     2221 cggccggccc ggctctacgt cgaccgggtc ttcacgatga aggggttcgg gcccgtcgtc
     2281 accggcacgc tcgactccgg ccgggtgcgg gtcgaggacc gcctggcgct ccttcccgag
     2341 gggcgcgagg tgcgcgtccg gcgcctcgag gtgcacggcg aggagcgcga ctcggcggaa
     2401 gccggggagc ggacgagcct gaacctggcc ggcgtcgaga ccgcggacct cgggcgcggg
     2461 agggcgctcg tcgccccgga gagcctcgag gcggcgacgc tcctgacggt cgaggtgacc
     2521 gcgctcccgg acctgggcgc cccgatcgag gacgggctgg ccgtgcgcct ccacaccggc
     2581 accgccgacg tcgcggcgcg gctgcacctc ctcggcggag gggacggcgc cccgacgctc
     2641 gcgccggggg agcgcgccgt cgcgcaagtc tccgctctcg agcccgtcgc cgcccgtcgc
     2701 ggggaccgct tcgtccttcg aaggccgtcc ccgcaggaga cgatcggcgg cggccgggtc
     2761 ctcgacgtcg ggcgcccgcg cgtgcggcgg cgcgcgccgc tctccgctca tgccgcctcc
     2821 gtcctggccg gcgacgacga gcgcgccgtc gccgcgctcc tcctctccga ggccggtccc
     2881 gccgggatcg acgtcggcat gctggcccgg cgcctcggcg tccccgccgc gaccgcggcg
     2941 gcccacctcg acgccctcgt cgcgagcggc gcggcacttc ggctcacacc cgcgctcgcc
     3001 gtctccgcgt cggcctcggt cgagatggcg acccgcgccg acgccttctt cgccgagcgg
     3061 cggaaggccg gggtcccgac cctcctcgtc ggccggcgcg agctcctcgg aaagattgcg
     3121 cgcggcctcc cggaagcgac ggccgaggcg tggctcgcga cgctcgccgc cgcgaggcgg
     3181 ctcgtcgtca ccgccgacca ggccggaccg ccgggcgccg ccgcctcggc cgtggcggac
     3241 gaggccgggg ccttcgcgtc ccggatcgag gaggcgtggc ggacggcggg gttcgacgcc
     3301 cccgggaacc tcgacctcgc gaaggcgctc ggcacgaagc cgcaggtcgt cgccgggctc
     3361 gtgcagcacc tcctgaagag cggggcgctc gtccgcctct ctcccgaggt tttcgtccac
     3421 gccgagctcc tcggcgggat cgaggcgaag gtggcggagc agaaggggcg gacgatgagc
     3481 gtcggcgact tccgcgacct tctcggcctc tcgcggaaat acctgattcc gctcctcgag
     3541 cacctcgacc ggaagaaggt cacgcggcgc gtcggcgacg cgcgcgtggt cgtctgatca
     3601 gcggcccgga acgcgcccct cctcctcgag ccgtccgagg aggcggagga ggccgtcgag
     3661 gatcg
//
LOCUS       DAOTEZ010000432         3648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_19046_length_3648_cov_4.185917, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000432 DAOTEZ010000000
VERSION     DAOTEZ010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19046_length_3648_cov_4.185917"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="P5164_17280"
     CDS             <1..215
                     /locus_tag="P5164_17280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45687.1"
                     /translation="KLATSIRERAPEDLEALAAAGVVAAGVFPTFEEMTEAAPRLGLP
                     VPTRERWAELRRDAFAAAALANQTEH"
     gene            506..1516
                     /locus_tag="P5164_17285"
     CDS             506..1516
                     /locus_tag="P5164_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45688.1"
                     /translation="MPINWETDLIVPQSVLDAIRVLQSQRSPFFSSGVAVLEEIDAFG
                     RGGNFVKRKYSAEDSVHGTRITGDEQVPSRLGAHADVAPVLRRLRLRTFIDGEGAAEG
                     GLMSEDPGGRILQATISWWTRETDEVLLACLAGAFGTGGCLEATHLHDVTGSGVAPGK
                     LTYSGVMDAAARLGDAAADLALVVMHSRQYLDVVKEAGARASSVPVGSEPFMTETFVG
                     PYKVVVSDAVPVEGTAGAADAIYTAILCGAGSFWSGVQQDLREFGGWNARVPRFELSQ
                     SRHVAVGITGLSASSAMPVNPTNEQLATAANWEPTVSLATEAGRKSVRVVGIKSGASP
                     AA"
     gene            1831..2925
                     /locus_tag="P5164_17290"
     CDS             1831..2925
                     /locus_tag="P5164_17290"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRY45689.1"
                     /translation="MPLKQDARGRWVFRYYADGVRTGRRVQVTLPPGTTHAEALTEYH
                     KRMAGAAARRGKPSLVRLTLEQAVDEFLRERKPHLSPVTLARTEQILRREWLPRFGQK
                     RVDALTAADVARYQTERLADGVKASTINLEVYRLKNVLVRLVAWGWIEQNPLRPKAVQ
                     MLPAPERRTDFFSPEEWKEFIGKVSAEAVPILRAALYTANRIGELCALRWGQVDLEGR
                     RIRVEMPKVRRTKVLMISRALYDVLSALPKGTPLAHVFTRADGSPWEPHSVGRAFRMA
                     RPRPELSVHSIRHTAASWLAIAGTPLRTIAAILGHTQLAMTFRYAHLSPEAVSDAVDV
                     IPEMERGGVGTPRRHPDAGKTLAAEGLLPS"
     gene            complement(2931..>3648)
                     /locus_tag="P5164_17295"
     CDS             complement(2931..>3648)
                     /locus_tag="P5164_17295"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HRY45690.1"
                     /translation="LDEELVLSDYVLLEKRREKLEKDRKKTKSAELDAEWAAIERCLP
                     ALESGTPLRAAGLTAEEEKRLRGFTLLSLKPLLIVVNVSDADASAEPVARFGLESFAA
                     QPHVAVARAAAKIEMELNDLPDEDVPVFLADLGLTERGVDRILRASYRLLGVQSFFTA
                     GEDECRAWTVPVGATAVECAGTIHSDLARGFIRAEVVPVEDLLDLGSLAECRTKGKLR
                     LEGKEYVVRDGDVVHVRFNV"
BASE COUNT          527 a         1340 c         1274 g          507 t
ORIGIN      
        1 ggaagctcgc gacgtcgatc cgcgagcggg cgcccgagga cctcgaggcc ctcgcagcgg
       61 ccggcgtggt cgcggccggc gtcttcccga cgttcgagga gatgaccgag gcggcgcccc
      121 ggctcggtct ccctgtccct acgcgcgagc gctgggccga gctccggcgc gacgccttcg
      181 ccgcggccgc cctcgcgaac cagacggagc actgacgatg ccttgcgccc ggaccccggc
      241 gcctgccggg tccattcctt cccggccgcg atccaacacc gagtcgtcct ttctcggcgc
      301 gatccaggtc ccggggtccg ggcctttctc tctcgggtcc ggttgtgccg ggccggtgtc
      361 cttcggcggt cggttgtgcc gaccgcgcgc gagctccgga ggggcggttg tgccgcccgt
      421 gcgccggggc ggaccgcgct cgaccgaaac cccgatcgac aacccgcacg aacccggcgg
      481 cctgacggcc gcctgaggac acaccatgcc catcaactgg gaaaccgacc tcatcgtccc
      541 gcagagcgtc ctcgacgcga tccgggtcct tcagagccag cgctcaccgt tcttctcgtc
      601 cggcgtcgcc gttctcgagg aaatcgacgc cttcggccgg ggcggaaatt tcgtcaagcg
      661 caagtactcc gccgaggaca gcgtccacgg cacccgcatc acgggagacg agcaggtgcc
      721 gtcccggctc ggagcacacg ccgacgtcgc tccggtcctt cggcgcctcc gcctccggac
      781 cttcatcgac ggcgagggcg cggccgaggg cgggctcatg tccgaggacc cgggcggccg
      841 aatcctccag gcgaccatca gctggtggac gcgcgagacg gacgaggtcc tcctcgcctg
      901 cctggccggt gcgttcggca cgggcggctg cctcgaggca acgcacctcc acgacgtgac
      961 cggctccggg gtcgccccgg gcaagctcac ctactccggc gtcatggacg cggccgcccg
     1021 cctgggcgac gccgcggccg acctggcgct cgtcgtcatg cactcgcggc agtacctcga
     1081 cgttgtgaag gaggccggcg cccgcgcgtc gtccgtgccg gtcggctccg agccgttcat
     1141 gaccgagacc ttcgtcggcc cgtataaggt cgtcgtgagc gacgcggtcc ccgtcgaggg
     1201 gacggccggc gcggcggacg cgatctacac cgcgatcctc tgcggggcgg gcagcttctg
     1261 gtccggtgtg cagcaggacc tccgggagtt cggcggctgg aacgcgcggg ttccccggtt
     1321 cgagctctcg cagtcccggc acgtcgccgt gggcatcacc ggcctctcgg cgtcgtccgc
     1381 catgccggtc aacccgacaa acgagcagct cgcgacggcc gcgaactggg agccgaccgt
     1441 gagcctcgcg accgaggccg gccggaagtc cgtccgggtc gtcggcatca agtcgggcgc
     1501 ctcgcccgcg gcctgacctt caccggccgg gggcggtccg ccgtccccgg ccttcccctt
     1561 catcgaggag gcgtgccgtg gggcgagcga ccgacgagac gacctcgggc ccggcccgcg
     1621 cgccgcgggg ctcctcgagg cgcctggggc ccccggcccc cccccccagg aaggccccga
     1681 gggggtccga aggaagcgcc ccagccctcc acgcgcgcgt ttcgaaacac gatgacgcca
     1741 aacccccagc gtatcgaaac gagccgcgct cccgcgggaa cccttgagca cgtaacgcct
     1801 tgagcgtttc gaaatgagaa ggaggaaccg atgcccctga agcaggacgc gcgcggccga
     1861 tgggtcttcc gctactacgc ggacggcgtc cggacgggcc ggcgcgtcca ggtgacgctc
     1921 ccgcccggga cgacccacgc ggaggccctg acggagtacc acaagcggat ggccggggcc
     1981 gcggcacgac gggggaagcc gagcctcgtc cggctcacgc tggaacaggc cgtggacgag
     2041 ttcctccggg agcggaagcc gcacctctcg cccgtgacgc tcgcccgaac ggaacagatc
     2101 ctccggcggg agtggctccc gcgtttcggc cagaagcgag tggacgcgct cacggccgcc
     2161 gacgtcgccc gataccagac ggagcgcctc gccgacggcg tgaaggcgtc gacgatcaac
     2221 ctcgaggtct accggctgaa gaacgtcctc gtccgtctcg tcgcctgggg atggatcgag
     2281 cagaacccgc tccggcccaa ggccgtccag atgctcccgg cgcccgagag gaggacggac
     2341 ttcttctcgc cggaggagtg gaaggaattc atcggcaagg tctcggccga ggccgttccg
     2401 atcctgcggg ccgccctcta cacggcgaac cgcatcggcg agctttgcgc gctccgctgg
     2461 ggtcaggtcg accttgaagg ccggcgaatc cgcgtcgaga tgccgaaggt ccggaggacg
     2521 aaggtcctca tgatctcgag ggccctctac gacgtcctgt ccgcgctccc gaagggaacc
     2581 ccgctggcac acgtcttcac tcgggccgat gggtcgccct gggagccgca cagcgtcggg
     2641 agggccttcc ggatggcgcg gccgcggccg gagctcagtg tccattcgat cagacacacg
     2701 gccgcgtcct ggctcgcgat cgcggggacg ccgctccgga cgatcgccgc catcctcggg
     2761 cacacccagc tggcgatgac gttccggtac gcgcacctct caccggaggc cgtttccgac
     2821 gcggtcgacg tcatcccgga gatggaaaga gggggggtcg ggacacctcg tcgccacccc
     2881 gacgccggga aaacccttgc ggctgagggc cttcttccct cttgacgtcg tcagacgttg
     2941 aagcggacgt ggacgacgtc gccgtcgcgg acgacgtact ccttcccctc cagccgcagc
     3001 ttccccttcg tacggcactc ggccagcgag cccaggtcga ggaggtcctc caccggcacg
     3061 acctccgcgc ggatgaagcc gcgcgccagg tcggagtgga tcgtcccggc gcattcgacg
     3121 gcggtcgcgc cgacggggac ggtccaggcg cggcactcgt cctccccggc cgtgaagaac
     3181 gactggacgc cgaggaggcg gtaggaggcg cggaggatcc ggtcgacgcc cctctcggtg
     3241 aggccgaggt cggcgaggaa gaccggcacg tcctcgtcgg ggaggtcgtt cagctccatc
     3301 tcgatcttcg cggcggcgcg ggccacggcg acgtgcggct gcgcggcgaa ggactcgagg
     3361 ccgaagcgcg cgaccggctc cgccgaggcg tcggcgtccg agacgttcac gacgatcagc
     3421 agcggcttga gcgagaggag cgtgaagccg cggagccgct tctcctcctc ggcggtcagg
     3481 cccgccgccc ggagcggagt cccggactcc agggcgggca ggcaccgctc gatcgccgcc
     3541 cactcggcgt cgagctcggc ggacttcgtc ttcttccggt ccttctcgag cttctcgcgc
     3601 cgcttctcga ggaggacgta gtccgagagg accagctcct cgtcgagc
//
LOCUS       DAOTEZ010000433         3634 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_19150_length_3634_cov_6.589271, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000433 DAOTEZ010000000
VERSION     DAOTEZ010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19150_length_3634_cov_6.589271"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="P5164_17300"
     CDS             <1..497
                     /locus_tag="P5164_17300"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY45691.1"
                     /translation="KVAVYTSTLGKAMGSAAGGFTTGPAPLVELLRQRSRTTLFSNAL
                     PPAVTASSLEAFRMLMEDPSPVKRLRANAAHFRTKMTEAGFNIPKGIHPIVPVIVGDT
                     AKALAMSAALFEKGVYVSGFGFPVVPQGHARLRCQISAVHSTADLDEAVDAFVAVGKE
                     FGVI"
     gene            complement(758..1699)
                     /locus_tag="P5164_17305"
     CDS             complement(758..1699)
                     /locus_tag="P5164_17305"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRY45692.1"
                     /translation="MSENPEVPSPEAPGPAPPDPSRADRARKGLLVFFALVALGSGIF
                     QGLILRSGKPIGESPWLVYSLMWTPGIASIVARLVFREGFRDVSFRIGGAAGWKAIGL
                     AWLLPPLVGFVAYGLAWGTGLAPFGIPALAEVGLSALPPAARFGVLFALSMTLVSLLS
                     FLSAVGEEIGWRGYLLTRLVAAEVPRPVLLSGVVWAVWHLPLVVSGQYAAGPSRALSA
                     VVFVLTVVGIGFVMGWMRLTTGSVLPAVVLHAVWNAVIQGTFDTTTKGPSMWVGEAGI
                     LVAAAALAAAWLLARKPYAARRAPGEEPFATIPGLAG"
     gene            complement(2021..>3634)
                     /locus_tag="P5164_17310"
     CDS             complement(2021..>3634)
                     /locus_tag="P5164_17310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HRY45693.1"
                     /translation="FPESVAASEIHKAGQRGADAAIQLFKAMGVGALIKILGDSGVFR
                     ASNEFHVAVGKFKESFVRLGLSKDANAIPAGGVTTIAAPAATPAYMGVGYIIGPELGA
                     LNFAGGLLAWGLFVPLLVFFLGPSLIDKYTDAATGAQDWAGLAGHLYRYIVRPIAVGG
                     MLVGACFTLWKMRKNLISGIKRGVADVKKSAAAAEATERTEKDLSFKVVLLGIAAVFV
                     LMVALYYYFTGVVGGALLAAVVMIITGFFFAAVSGNLVGLIGSSNNPISGLTLATTIV
                     AALTMVIIGVKGAEGVAAVLAVAAVVCVSSAVAGEMLQDLKVGHILGGTPWKMQVGDV
                     IGVVIAGAVMFFPLYLLHTSDLASNPGVGGFGGKNLPAPQAGLMAALSQGIVGGEMAW
                     PLVLVGIAMGITLILVRVKSPMLFSVGMYLPLETTFAIFIGGLIRGFVDKLAGKRDLN
                     VAQKARVENAGVLAASGLIAGEALVGLLVAGVVAFRSDNKFPQIEAFAGIAPWLAIPV
                     VALLAAYLIYVPLGKAGAADEPAPPTAVM"
BASE COUNT          587 a         1312 c         1280 g          455 t
ORIGIN      
        1 ggaaggtcgc cgtctacacc tcgacgctcg gcaaggcgat gggctcggcc gccgggggct
       61 tcacgaccgg cccggcgccg ctcgtcgagc tcctccgcca gcgctcgcgg acgacgctct
      121 tctcgaacgc cctcccgccc gccgtcaccg cctcctcgct cgaggcgttc cggatgctca
      181 tggaagaccc gtctcccgtg aagcgtctcc gagcgaacgc cgcgcacttc cggacgaaga
      241 tgaccgaggc gggcttcaac atcccgaagg ggatccaccc gatcgtcccg gtcatcgtcg
      301 gcgacacggc gaaggccctt gcgatgtcgg ccgccctctt cgagaagggg gtctacgtct
      361 ccggcttcgg cttccccgtc gtcccgcagg ggcacgcccg cctccgctgc cagatctcgg
      421 ccgtccactc caccgccgac ctcgacgagg ctgtcgacgc cttcgtcgcc gtcggaaagg
      481 agttcggagt catctgacga ctctcgcggg gggaccacgg cccgccgggc cagcggccgg
      541 tccccccgca caccctccga gccgacagcc cccgtgcggt cgcgcccatg gtggtggcac
      601 cgtgaggggc ccccttctgg ggccacaccc cttctggcgc caggcgtgaa atcgacccct
      661 cgccctctgg tgccaggcgt gaaatcgtgt attgtctaag acaatacacg atttcacgcc
      721 tggcaccaat cccggggcac caatcccgga ccccgggtca gccggcgagg ccggggatcg
      781 tcgcgaacgg ctcttcgccg ggcgcgcggc gggcggcgta gggcttccgg gcgaggagcc
      841 aggcggcggc gagcgccgcg gcggcgacga ggatccccgc ctccccgacc cacatcgacg
      901 gccccttcgt cgtcgtgtcg aacgtcccct ggatcaccgc gttccagacc gcgtggagga
      961 cgaccgccgg caatacgctc ccggtcgtca gccgcatcca gcccatcacg aacccgattc
     1021 cgacgaccgt gaggacgaag acgaccgccg agagcgcccg gctcggcccc gccgcgtact
     1081 gcccgctcac gacgagcgga aggtgccaga cggcccagac gacaccgctc aggaggaccg
     1141 gccgcggcac ctcggccgcg acgaggcgcg tcaggaggta gccgcgccat ccgatctcct
     1201 ctccgaccgc cgagaggaag gacaggagcg agacgagagt catcgagagg gcgaagagga
     1261 ccccgaaccg ggccgcgggc gggagggccg agagcccgac ctccgcgagc gccgggatcc
     1321 cgaacggcgc gaggccggtc ccccaggcga ggccgtacgc gacgaagccg acgagcggcg
     1381 gaaggagcca cgcgagtccg atcgccttcc accccgcggc gccgccgatc cggaacgaga
     1441 cgtcgcggaa gccctcccgg aagacgagcc gggcgacgat cgaggcgatc cccggcgtcc
     1501 acatcaggct gtagacgagc cacgggctct ccccgatcgg cttccccgac cggaggatca
     1561 gcccctggaa gatgccgctt ccgagcgcca cgagcgcgaa gaagacgagg agccccttgc
     1621 gggcccggtc ggcgcgggac ggatcgggcg gcgcggggcc gggcgcctcg ggcgacggga
     1681 cttccgggtt ctcgctcacc cggcgagtct cccacgacac cgggttcggt gccaggcgtg
     1741 aaatcgtgta ttaccggagg gcgcctcacc tgcagccgcg accggcgatc actcttccgc
     1801 ggccgcggcc gcgagcgcct ccgcgtcgag gaggtccttc ggccggccgg tggcccgcgg
     1861 gacgccccgc ggagagagcg atccgcttca cgggccagga cgacctctcc gcgcggccgc
     1921 tcacgaagac gattccattc cccggcgtcg tgagcgccca tcgacacgaa ggccgcggct
     1981 tgcgccgcgg ccttcgaccg gaacgtgggg aaaaccgggg ctacatgacc gccgtcggcg
     2041 gggccggctc gtcggccgcg cccgccttgc cgagcgggac gtagatcagg tacgccgcga
     2101 ggagggcgac gaccgggatc gcgagccagg gggcgatccc cgcgaacgcc tcgatctgcg
     2161 ggaacttgtt gtcggagcgg aacgcgacga cgccggccac gaggaggccg acgagcgcct
     2221 cgcccgcgat caggccggac gccgcgagga cgcccgcgtt ctccacgcgc gccttctgtg
     2281 ccacgttgag gtcgcgcttg ccggcgagct tgtcgacgaa gccgcggatg aggccgccga
     2341 tgaagatcgc gaacgtcgtc tcgagcggca ggtacatgcc gaccgagaag agcatcgggc
     2401 tcttgacgcg gacgaggatc agcgtgatcc ccatcgcgat gccgacgagg acgagcggcc
     2461 aggccatctc gccgccgacg atcccctgcg agagggccgc catgaggccg gcctgcgggg
     2521 ccgggaggtt cttgccgccg aagccgccga cgcccgggtt cgacgccagg tccgacgtgt
     2581 ggaggaggta gagcgggaag aacatgacgg cgccggcgat gacgacgccg atgacgtcgc
     2641 cgacctgcat cttccacggg gtgccgccga ggatgtgccc gaccttcagg tcctggagca
     2701 tctcgcccgc gacggcggag gagacgcaga cgaccgcggc cacggcgagg accgccgcga
     2761 cgccctcggc gcccttgacg ccgatgatga ccatcgtgag cgcggcgacg atcgtcgtcg
     2821 cgagcgtgag gcccgagatc gggttgttcg acgagccgat gagcccgacg aggttccccg
     2881 agacggccgc gaagaagaag ccggtgatga tcatgacgac ggccgccagg agcgcgccgc
     2941 cgaccacgcc ggtgaagtag tagtagaggg cgaccatcag gacgaagacg gccgcgatcc
     3001 cgaggaggac gaccttgaag gagaggtcct tctcggtccg ctcggtcgcc tcggccgccg
     3061 ccgccgactt cttcacgtcg gccacgccgc gcttgatgcc cgagatgagg ttcttccgca
     3121 tcttccagag cgtgaagcag gcgccgacga gcattccgcc gaccgcgatc ggccggacga
     3181 tgtagcggta gaggtggccc gcgaggcccg cccagtcctg ggcgccggtc gccgcgtcgg
     3241 tgtacttgtc gatcaggctc gggccgagga agaagacgag gagcgggacg aagagccccc
     3301 acgcgaggag gccccccgcg aagttcagcg cgccgagctc cggcccgatg atgtagccga
     3361 cgcccatgta ggcgggcgtg gcggccgggg ccgcgatcgt cgtcacgcct ccggccggga
     3421 tcgcgttcgc gtccttcgag aggccgagcc tcacgaagct ctccttgaac ttcccgaccg
     3481 cgacgtggaa ctcgttcgag gcgcggaaga ccccggagtc gccgaggatc ttgatgaggg
     3541 cgccgacgcc catcgccttg aagagctgga tcgccgcgtc ggccccgcgc tgacccgcct
     3601 tgtggatctc gctcgcggcg accgactcgg ggaa
//
LOCUS       DAOTEZ010000434         3600 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_19419_length_3600_cov_5.506629, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000434 DAOTEZ010000000
VERSION     DAOTEZ010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19419_length_3600_cov_5.506629"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="P5164_17315"
     CDS             <1..551
                     /locus_tag="P5164_17315"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HRY45694.1"
                     /translation="GLLIGLLVVGVQWWVEASRLKEQQEKNRIAQQEVTRLEAVLKEV
                     ADFEDKKAKLQKKVDLINALKANQRGPVRLMDEVSNALPDLLWIERMQVTGNSITIDG
                     KALNPPAVANYLENLKRVSAFQEPKVTAITAASAGASNLYNFGLTFVFADLDRTQFVS
                     GAPAGGEPAPAPAAPAAPAAGN"
     gene            562..1233
                     /gene="pilO"
                     /locus_tag="P5164_17320"
     CDS             562..1233
                     /gene="pilO"
                     /locus_tag="P5164_17320"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HRY45695.1"
                     /translation="MAIELKQLEDKPWYYGLGIGLALAVGLWAVANWQYPNFKEMQQR
                     LAGLKAEYGQLQEKIERGRVAERRLPQLREEVRRIELDLNRLLEILPTKRNTEELIKK
                     IEALTRQGDFFLKDFRPKEYVNKDFYAEWPIDVVLDGTYHNLALFFDKMARFSRIINV
                     ESLTMTGYPDARLGRTLGATFTLKTFIYLGDQASGPGATAPPGGAAPAAPAAIPSGAS
                     REGGV"
     gene            1233..1739
                     /locus_tag="P5164_17325"
     CDS             1233..1739
                     /locus_tag="P5164_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45696.1"
                     /translation="MKLPCSSAGTILAAASLLVLASPALGQEPAPPPTTPPAETGIAV
                     PVPGEQDTSAPAYTYEVRGRRDPFRSLLLRTQDDKDRQRPPGIAGMLVEELELQGTIR
                     TRQGWIAMMRGGDNRSYLLRKGTTVFDGEVMEIGPTEIVFRQNVNDPTSPKPFRDVVK
                     ALALQAKP"
     gene            1826..>3600
                     /locus_tag="P5164_17330"
     CDS             1826..>3600
                     /locus_tag="P5164_17330"
                     /inference="COORDINATES: protein motif:HMM:NF023169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HRY45697.1"
                     /translation="MFRTGRFSAPGLAVILVAALAAAGCSSQAGPAPTTENLASASAV
                     PSVLTSAALLIDGDAPRLLLAADGPLRPMVYAREGSKTLVVDLPGTVVTPGLEPPRAD
                     GTLLTALEMRSFSELGQPQVRFELTGRGALDAQLAGDPAARAMSIALLPRIEEPVVAQ
                     STPPAPSEAAPQAAAPVAAAPAATSTEAVVVARHEAAGAAATRLARVATQARDGALSV
                     RLEADGEISYEAFTLPSPPRFVVDLSGVRNASRFRAEDVDAAGVARVRVSQFRTEPTA
                     VTRVVFDLEKESLPVLRQSGSTVSISFGGATEPPVQVASAVPVAPAAGSYEAHEADDA
                     SEPVVPPAAVPEPVAATPTAPEPVVPVAEAPEVRVAETAPAAPPAAAEPPAASEPVAT
                     AALSAHEPVVVSEPEPARVASAPAPTVTVASPEVAVAAPAATRPARRKTSADDRALLE
                     AAETLAAEQQGSGQLKDISNPYESRAIGQTERQYTGEPLTLNLKDADIKDTLQKFSEL
                     TNLNIVLDPDVRGTVTVSLTDIPWDQALELILKINGLGYVLEGNVMRIAATGKLASEE
                     AARQSLLKAQESNRPTRTVIQKLSYA"
BASE COUNT          552 a         1352 c         1225 g          471 t
ORIGIN      
        1 gagggctcct catcgggctc ctcgtcgtcg gcgtccagtg gtgggtcgag gcgtcgcgcc
       61 tgaaggagca gcaggagaag aaccggatcg ctcagcagga ggtcacccgg ctggaggccg
      121 tcctgaagga ggtcgccgac ttcgaggaca agaaggcgaa gctccagaag aaggtcgacc
      181 tgatcaacgc cctcaaggcg aatcagcgcg ggccggtccg gctgatggac gaggtctcca
      241 acgccctccc ggacctcctc tggatcgaga ggatgcaggt gacgggcaac agcatcacga
      301 tcgacggaaa ggcgctcaac ccgccggccg tcgcgaacta cctcgagaac ctcaagcggg
      361 tctcggcgtt ccaggagccg aaggtcacgg cgatcaccgc cgcgagcgcc ggcgcctcga
      421 acctctacaa cttcgggctg accttcgtat tcgcggatct cgacaggacc cagttcgtat
      481 ccggcgcgcc ggccgggggc gagcccgctc ccgcgcctgc cgctccagcc gcgccggccg
      541 cggggaactg agggggccga cgtggcgatc gaactgaagc agctcgaaga caagccctgg
      601 tattacggcc tcggcatcgg cctcgcgctc gcggtcgggc tctgggccgt cgcgaactgg
      661 cagtacccga atttcaagga gatgcagcag cggctcgccg gcctgaaggc cgagtacggg
      721 cagctgcagg agaagatcga gaggggccgg gtggccgagc ggaggctccc gcagctgcgc
      781 gaggaggtcc ggcggatcga gctcgacctg aaccgcctcc tcgagatcct gccgacgaag
      841 cggaacaccg aggagctcat caagaagatc gaggccctca cgcggcaggg cgacttcttc
      901 ctgaaggact tccggcccaa ggaatacgtc aacaaggact tctacgccga gtggccgatc
      961 gacgtggtcc tcgacgggac gtaccacaac ctggcgctct tcttcgacaa gatggcccgc
     1021 ttctcccgga tcatcaacgt cgagtcgctg acgatgacgg ggtatccgga cgcgaggctc
     1081 ggccgcacgc tcggggcgac cttcactctg aagacgttca tctacctcgg cgaccaggcg
     1141 tccgggcccg gggccaccgc acctccgggc ggggcggcac cggccgctcc cgctgcgatc
     1201 ccgtccggcg cgagccgcga gggaggcgtc tgatgaagct gccgtgctcc tcggcgggga
     1261 cgatcctcgc cgccgcctcg ctcctcgtcc tcgcctcgcc cgcgctcggg caggagcccg
     1321 cgcccccgcc gacgacgccg ccggcggaga cggggatcgc cgtcccggtt ccgggtgagc
     1381 aggacacctc cgccccggcc tatacctacg aggtccgcgg gcgccgcgac ccgttccgat
     1441 ccctcctgct tcggactcag gacgacaagg accggcagag gccccccgga atcgccggga
     1501 tgctcgtcga ggagctggag ctccagggga cgatccggac ccgtcagggg tggatcgcga
     1561 tgatgagggg cggcgacaac cgctcgtacc tgctccggaa gggaaccacg gtcttcgacg
     1621 gcgaggtcat ggagatcggc ccgaccgaga tcgtgttccg ccagaacgtc aacgacccga
     1681 cgagcccgaa gcccttccgg gacgtcgtca aggcgctggc cctccaggcg aagccgtgag
     1741 cgaaggaagt tcgatgacga tcaagacgac ttgcctcgaa cccggtcggc ccctcggcac
     1801 ctcgaacctc tcgtggggag gaaccatgtt tcgaactgga aggttctcgg cacccgggct
     1861 cgcggtcatc ctcgtcgcgg ccctcgcggc cgcgggctgc tcttcgcagg ccggacccgc
     1921 accgacgacg gagaacctcg cctccgcgtc ggcggtcccg tcggtcctga cgagcgccgc
     1981 gctcctgatc gacggggacg cccccaggct cctgctggcg gcggacggcc cgctgcggcc
     2041 gatggtctac gctcgcgaag ggtcgaagac cctcgtggtc gacctccccg gcacggtcgt
     2101 cacgcccggc ctcgagccgc cgcgcgccga cggcacgctc ctgaccgcgc tcgagatgcg
     2161 gtcgttctcg gagctcggcc agccccaggt ccgcttcgag ctgaccgggc gcggcgcgct
     2221 cgacgctcag ctcgccgggg acccggcggc ccgcgcgatg tcgatcgcgc tcctcccgcg
     2281 gatcgaggag ccggtcgtcg cgcagtccac gccgccggcg ccttccgagg ccgctccgca
     2341 ggccgccgcc ccggtcgccg ccgcacccgc ggcgacgtcg acggaagcgg tcgtcgtcgc
     2401 ccgccacgag gcggctggcg ccgccgcgac ccgcctcgcg cgcgtcgcga cgcaggcccg
     2461 ggacggcgcg ctctccgtcc ggctcgaggc cgacggagag atctcctacg aggcgttcac
     2521 gcttccgagc ccgccgcgct tcgtcgtcga cctctcgggc gtgcggaacg cgtcgcgctt
     2581 ccgtgccgag gacgtcgatg ccgccggcgt cgcccgggtc cgcgtctcgc agttccggac
     2641 cgagccgacg gcggtcacgc gcgtcgtctt cgacctcgag aaggaatccc tcccggtcct
     2701 ccgtcagagc ggttcgaccg tctcgatctc gttcggcgga gcgacggagc ccccggtgca
     2761 ggtcgcgtcc gccgttcccg tcgctccggc cgcgggctcg tacgaggcgc acgaggccga
     2821 cgacgcctcc gagccggtcg tcccgcccgc cgcggtcccc gagccggtcg cggcaacacc
     2881 gacggctccc gagcccgtcg tcccggtcgc cgaggccccc gaggtccgcg tcgccgagac
     2941 ggcgccggcc gccccgccgg ccgccgccga gccccccgcc gcttcggagc cggtcgcgac
     3001 ggcggcgctt tcggcccacg agcccgtcgt cgtgtccgag ccggagccgg cgcgcgtcgc
     3061 ctctgcgccc gctccgaccg tgacggtcgc ctcccccgag gtcgccgtcg cggcgcccgc
     3121 cgcgacgcgc cccgcacgac ggaagacctc ggcggacgac cgcgcgctcc tggaggccgc
     3181 cgagaccctg gccgcggagc agcagggcag cggccagctg aaggacatct cgaacccgta
     3241 cgagtcccgg gcgatcggcc agacggagcg tcagtacacc ggcgagcccc tgaccctgaa
     3301 cctcaaggac gccgacatca aggacacgct ccagaagttc agcgagctga cgaacctgaa
     3361 catcgtcctc gacccggacg tgcgcggcac cgtcaccgtc tcgctgaccg acatcccctg
     3421 ggaccaggcc ctcgagctga tcctcaagat caacgggctc ggctacgtcc tcgaggggaa
     3481 cgtcatgcgg atcgccgcca cgggcaagct cgcctccgag gaagcggccc gccagagcct
     3541 cctcaaggcg caggagtcca accggccgac ccggaccgtg atccagaagc tctcgtacgc
//
LOCUS       DAOTEZ010000435         3599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_19426_length_3599_cov_7.073363, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000435 DAOTEZ010000000
VERSION     DAOTEZ010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19426_length_3599_cov_7.073363"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="P5164_17335"
     CDS             complement(<1..343)
                     /locus_tag="P5164_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008717848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRY45698.1"
                     /translation="MKKTITLDVNGVAETAEVEVGQTLLRFLRDGLDLTGTKEGCNEG
                     ECGSCMVLLDGRPVNGCLVLAVEADGRSVTTVEGLSKEGLLHPLQRAFLSQAAVQCGF
                     CTPGMVVSAAAL"
     gene            complement(354..1193)
                     /locus_tag="P5164_17340"
     CDS             complement(354..1193)
                     /locus_tag="P5164_17340"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HRY45699.1"
                     /translation="MPVDLDYLSPSAVDDAVALRASRPSAAFLLGGTDLLPQMRSGRR
                     SPETLIDLKRIPELHEIRENEDGGLSVGAAVPLADVETHPVVLSRFPLLAECSKTVGA
                     WPLRQRATLAGNVCNASPAADTAVALLALDAVVNVASTTGRLAMAISELFLGPGQTAL
                     MAGDLVTEIVLPGAAAGFRGSYQRLSRRKGMDLATVGVLVGKRDGAPASWRVALAAVA
                     PTPLRVAAAEELLAAKGASAAREAAELAVAACRPITDLRGSAEYRREMVGVLVRRGVE
                     SLG"
     gene            1450..2745
                     /gene="uraA"
                     /locus_tag="P5164_17345"
     CDS             1450..2745
                     /gene="uraA"
                     /locus_tag="P5164_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HRY45700.1"
                     /translation="MERRTIDVDERLPLLQTVPLSLQHLFAMFGATVLVPFLFKVNPA
                     TSLLLNGVGTLVYLAVCRGKVPAYLGSSFAFISPVFAVLAHPELGGYAAAQGGFIAFG
                     LFFVLVSQVVRVAGTRWLDVVFPPAAMGAIVAVIGLELAPVATSMAGLTGDGAAQPGR
                     GTAVAVAMVTLAVTILGSVLFRGFLGVIPVLIGVAAGYGAALAAGIVDLSPVAAAPWL
                     EVPTLYAPAFSPQAILMILPAGLVVLAEHVGHLVVTGNVVGRDFVKDPGLHRSLLGDG
                     LSNVLSGLAGATPNTTYGENIGVLAITRVFSTWVIGGAAVLAVVISFVGKLAAAIRSI
                     PTPVMGGVCILLFGVIAAAGIRMLVEKKVDYTRPRNLVLTSVVLVSGASGAAVKLGAV
                     ELKGMALGTFVAVALSLLLALFDRLGWTNDGAATADAEG"
     gene            2749..>3599
                     /locus_tag="P5164_17350"
     CDS             2749..>3599
                     /locus_tag="P5164_17350"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HRY45701.1"
                     /translation="MRRAAALCCAAIALGSSVWPRAGPQPPSELRAVRPAVAADEAPF
                     YAEEFLNEESPGRMCHVASLCELSDGRLASVWYSGSAECAPDVTIEFASRAAGDGAAW
                     SAPRTIVTRESASRESGRFVKAVGNPVVFADDRDRLWLLHSAILLGGHGGSSLDVATS
                     DDGGATWGRRRRLTLSPFLNVSELARGRPVPLEGGGFAIPVYHELLGKFPEMLWLSAA
                     DSPSGLAWRKTRLAGGRSYLQPVVVPADAKNAVAFLRDRSRRRAVTMLRARDGRLHLV
                     YSWQMKR"
BASE COUNT          429 a         1409 c         1220 g          541 t
ORIGIN      
        1 ggagcgccgc ggcgctcacg accatccccg gcgtgcagaa gccgcactgg accgcggcct
       61 gcgagaggaa cgccctctgg agggggtgga ggaggccctc cttcgagagc ccctcgaccg
      121 tcgtcacgga gcgcccgtcc gcctccaccg cgaggacgag gcagccgttc acggggcggc
      181 cgtcgaggag gaccatgcag gagccgcact ccccctcgtt gcacccttcc ttcgtccccg
      241 tcaggtcgag gccgtcgcgg aggaagcgga ggagcgtctg cccgacctcg acctccgccg
      301 tctcggcgac gccgttcacg tcgagcgtga tcgtcttctt catctccgtc cccctacccg
      361 aggctctcga cgccgcggcg gacgaggacg cccaccatct cgcggcggta ctccgcgctt
      421 ccgcggaggt cggtgatcgg gcggcaggcg gcgacggcga gctccgccgc ctcccgcgcc
      481 gcggaggccc ccttcgccgc gaggagctcc tcggcggcgg cgacgcggag cggcgtcggc
      541 gccacggccg cgagcgccac gcgccacgag gccggcgctc cgtccctctt cccgacgagg
      601 acgccgaccg tcgcgaggtc catccccttc cggcgcgaga ggcgctggta cgacccgcgg
      661 aagcctgctg cggcgcccgg gaggacgatc tccgtcacga ggtcgcccgc catgagcgcc
      721 gtctggcccg gtcccaggaa gagctccgag atcgccatcg cgagccgccc cgtcgtcgag
      781 gcgacgttca cgacggcgtc gagcgcgagg agcgcgacgg ccgtgtcggc cgcgggggac
      841 gcgttgcaga cgttccccgc gagcgtcgcc ctctggcgga gcggccaggc cccgaccgtc
      901 ttcgaacatt ccgcgaggag cgggaagcgc gagaggacca ccgggtgcgt ctccacgtcg
      961 gcgagcggca cggccgcgcc gacggagagc ccgccgtcct cgttctcccg aatctcgtgg
     1021 agctcgggga tcctcttcag gtcgatgagc gtctcggggc tccgccgccc gctccgcatc
     1081 tgcggaagca ggtcggtccc gccgaggagg aaggccgcgg aggggcggct cgcccgaagc
     1141 gcgacggcgt cgtccacggc ggacggggag aggtagtcga ggtcgacggg catcagacga
     1201 tcccttccgc gacggaggcg accggacggc ccctcgcgcc cgctgtgcga tcgacggcgt
     1261 tcgttgaaaa cggcggtccc gaggggctcc cggaggagcc gcgcctcgca ggtgtcatcc
     1321 ggtgatcgta gtcccgcgta ccgggccggg ggaagcgccg accgccttcg ccgccgccgc
     1381 acgacccttc cgttctggcc tagaatggaa ggttgcgccg gggacggccg gcccgcgagg
     1441 aggacctcca tggagcgacg caccatcgac gtcgacgagc ggctccccct gctccagacg
     1501 gtccccctct ccctccagca cctcttcgcg atgttcggcg cgacggtcct cgtgccgttc
     1561 ctcttcaagg tcaacccggc gacctcgctc ctcctgaacg gcgtcgggac gctcgtctac
     1621 ctcgccgtct gccgcgggaa ggtgcccgcc tacctcggct cgagcttcgc cttcatctcc
     1681 cccgtcttcg ccgtcctcgc ccaccccgag ctcggcggct acgccgcggc gcagggaggc
     1741 ttcatcgcct tcggactctt cttcgtcctc gtgtcgcagg tcgtccgcgt cgccgggacg
     1801 cgctggctcg acgtcgtctt ccctcccgcc gcgatggggg cgatcgtcgc cgtcatcggt
     1861 ctcgagctcg cccccgtcgc cacgagcatg gccggactga ccggcgacgg cgccgcgcag
     1921 ccggggcgcg gcaccgcggt cgccgtcgcg atggtcactc tcgccgtcac gatcctcggg
     1981 tccgtcctct tccgcggctt cctcggcgtc atcccggtcc tgatcggcgt cgccgccggc
     2041 tacggcgcgg ccctcgcggc cgggatcgtc gacctctccc ccgtcgccgc ggcgccgtgg
     2101 ctcgaggtgc cgacgctcta cgcccccgct ttctcgccgc aggcgatcct gatgatcctc
     2161 cccgccggcc tcgtcgtcct cgccgagcac gtcgggcacc tcgtcgtcac ggggaacgtc
     2221 gtcgggcgcg acttcgtgaa ggaccccggc ctccaccgct ccctcctcgg cgacggtctc
     2281 tcgaacgtcc tctcgggcct cgcgggcgcc acgccgaaca cgacctacgg cgagaacatc
     2341 ggcgtcctcg cgatcacccg cgtcttctcc acgtgggtca tcggcggcgc cgcggtcctc
     2401 gccgtcgtca tctccttcgt cggcaagctc gccgcggcga tccggagcat cccgaccccc
     2461 gtcatggggg gcgtctgcat cctcctcttc ggcgtcatcg ccgcggccgg catccggatg
     2521 ctcgtcgaga agaaggtcga ctacacgagg ccccgcaacc tcgttctcac ctctgtcgtc
     2581 ctcgtcagcg gcgcgagcgg tgccgccgtg aagctcggcg ccgtcgagct gaaggggatg
     2641 gccctcggca ccttcgtcgc cgtcgccctc tcccttctcc tcgccctctt cgacaggctc
     2701 ggctggacga acgacggggc cgccacggcc gacgccgaag gctgacgcgt gaggcgagcc
     2761 gcggccctct gctgcgccgc gatcgccctc ggctcgagcg tctggccgcg agccggcccg
     2821 cagccaccct cggagctccg cgccgtgcgt cccgccgtcg cggcggacga agcccccttc
     2881 tacgcagagg agttcctgaa cgaggagtcc cccgggagga tgtgtcacgt cgcctcgctc
     2941 tgcgagctct ccgacggccg cctcgcctcc gtctggtact ccggcagcgc cgagtgcgct
     3001 cccgacgtca cgatcgagtt cgcgtcgcgc gctgccggcg acggcgccgc ctggtcggcg
     3061 ccccgcacga tcgtcacgcg cgagtcggcc tcgcgggagt cggggcgttt cgtcaaggcc
     3121 gtcggcaacc ccgtcgtctt cgccgacgac cgtgaccggc tctggctgct ccactcggcg
     3181 atcctgctcg gcggccacgg agggagctcg ctcgacgtcg cgacgtccga tgacggcggc
     3241 gcgacgtggg gccggaggcg ccggctcacg ctcagcccgt tcctgaacgt cagcgagctg
     3301 gctcgcggcc gcccggtccc gctcgaagga ggcggcttcg ccattcccgt ctaccacgag
     3361 ctcctcggca agttccccga gatgctctgg ctctccgccg cggactcgcc gtcgggcctg
     3421 gcgtggcgga agacgcgcct ggccggcgga cgctcgtacc tccagccggt cgtcgtcccg
     3481 gccgacgcga agaacgccgt cgcgttcctt cgcgatcgct cgcggagacg ggcggtgacg
     3541 atgctccgcg cgcgcgacgg ccgcctgcac ctcgtctact cgtggcagat gaagcgcat
//
LOCUS       DAOTEZ010000436         3598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_19441_length_3598_cov_5.907705, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000436 DAOTEZ010000000
VERSION     DAOTEZ010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19441_length_3598_cov_5.907705"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="P5164_17355"
     CDS             complement(<1..915)
                     /locus_tag="P5164_17355"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase family protein"
                     /protein_id="HRY45702.1"
                     /translation="MAVADALRALVLRPKAELHLHLEGAVRPATLVRLARRAASPLFP
                     DLAAVRARGRPGSLAGFLSLYRDVCRLLREPDDFALLARDLTARLRRGRIVYAEVYVS
                     PAVVERVGFPWPAVRDVVERVFAAHERAGHGRIRVLLDAVRHFGPDAAHRVLDLQSLH
                     PWPRAVGFGLGGDELAVPARDFVSVYRRLAGLGLAPVVHAGETGGPDSVAEALRWLRP
                     RRIAHGVGAAADPSLVRRLARSGVALDVCLTSNAATGTHPLAGHPVLRLLRSGVAVTL
                     STDDPGLFGTTLLGEYRLLARLGATEREL"
     gene            859..1362
                     /locus_tag="P5164_17360"
     CDS             859..1362
                     /locus_tag="P5164_17360"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRY45703.1"
                     /translation="MELGLRAEHEGAEGVGDGHGIASYGRRAAEPTAVPSERLWLRAA
                     AFGLDLICLAGGPLLLATVVVFLVGLIAPDPPAGLPLVFRAAQALFVLLFLFRDVRGA
                     SPGKALLGLAVVRTDRGPLRARDSVLRNLPLLVPGLNLLEALSVVRRPDSRRLGDRLA
                     GTAVGES"
     gene            1415..2233
                     /locus_tag="P5164_17365"
     CDS             1415..2233
                     /locus_tag="P5164_17365"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HRY45704.1"
                     /translation="MDRFPRLPRHFLPLAALAVAALALTGGALAGQALEEPFEKTYDG
                     NGIERVRLQNVNGPVRVGTWERGHVRVSAVKRAKGSRAEEAIAQTEIRVTKRAGAIEI
                     ETVLPKPTRSWGFFSWGNRGGAEVAYELLLPAALPVDVETVNGRVTAEKRLGPVTLNT
                     VNGSVRVEEQGGPLHVNTVNGSVEVVFSGSLQTADLETVNGSVTVTCARDSSFRFDLQ
                     TVNGKIRSDFPDVTVEGKWGPKEARGEIAGGRERLTVETVNGEVRVLSGDAAAR"
     gene            2281..2652
                     /locus_tag="P5164_17370"
     CDS             2281..2652
                     /locus_tag="P5164_17370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45705.1"
                     /translation="MPEEVGAPAPPPPPPPPSVPPASSNRSVMLVLSYLGILALIPLL
                     TEKNDREVQWHAKHGLVLLVAYLVLMVGIMIASFVVGFFGMLQIPLWLGYLVVIILGI
                     TNALNGKRFVIPGVSEFAEKL"
     gene            complement(2742..2826)
                     /locus_tag="P5164_17375"
     tRNA            complement(2742..2826)
                     /locus_tag="P5164_17375"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2790..2792),aa:Leu,seq:gag)
     gene            complement(2895..3338)
                     /gene="secG"
                     /locus_tag="P5164_17380"
     CDS             complement(2895..3338)
                     /gene="secG"
                     /locus_tag="P5164_17380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRY45706.1"
                     /translation="MIYVLVPIYVIVCVFLILVVLLQQGKGADVASALGASSSQTTFG
                     ARGATTVLEKVTTWSFVAFSVLAVTISLIQARPKSSVIKQLGSQPVKSAPAAPAAPAP
                     ANAVPVAPAPGGAVPVAPAPASAVPAAPAAADAAPAAPAPAPAQK"
     gene            complement(3438..>3598)
                     /locus_tag="P5164_17385"
     CDS             complement(3438..>3598)
                     /locus_tag="P5164_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRY45707.1"
                     /translation="AAAAATRILYGGSVTPANAADLFAKPDIDGGLVGGASLVPADFA
                     ALAVAAGA"
BASE COUNT          524 a         1250 c         1358 g          466 t
ORIGIN      
        1 cagctcccgc tcggtcgccc cgaggcgggc gaggagccgg tactcgccga ggagcgtcgt
       61 cccgaagagg ccggggtcgt cggtcgagag ggtcaccgcg acgccggacc ggagcagccg
      121 gaggaccgga tgccccgcca gcggatgggt gccggtcgcc gcgttcgagg taaggcagac
      181 gtccagcgcg acgcccgacc gggcgaggcg tcggacgagc gacgggtcgg ccgcggcgcc
      241 gacgccgtgg gcgatccgtc gcggcctcag ccagcggagc gcctcggcga cggagtcggg
      301 accgcccgtc tcgccggcat gtacgacggg ggcgaggccg aggccggcga ggcgacggta
      361 gacggagacg aagtcccgcg ccgggacggc gagctcgtcc cccccgaggc cgaagccgac
      421 cgcgcgcggc caggggtgga gcgactgaag gtcgaggacc cggtgcgccg cgtcggggcc
      481 gaaatgccgg accgcgtcga ggaggacgcg gatgcggcca tgtcccgccc gctcgtgggc
      541 ggcgaagacc cgctcgacga catcgcggac ggcgggccac ggaaagccga cgcgctcgac
      601 gacggccggc gagacgtaga cctcggcgta gacgatccgg ccgcggcgga ggcgggccgt
      661 caggtcgcgc gcgaggaggg cgaagtcgtc cggctcgcgc aggaggcgac agacgtcgcg
      721 gtagagcgag agaaagccag cgagcgagcc ggggcgcccc cgggcgcgga cggccgcgag
      781 gtcggggaag aggggcgagg cggcgcggcg ggcgaggcgg acgagcgtcg cggggcggac
      841 ggccccttcg agatggagat ggagctcggc cttcgggcgg agcacgaggg cgcggagggc
      901 gtcggcgacg gccacgggat cgcatcctac gggaggagag cggcggagcc gacggcggtc
      961 ccgtccgagc gtctctggct gcgagcggcg gcgttcggcc tcgatctgat ctgcctcgcc
     1021 ggcggtccgc tccttctcgc gaccgtcgtc gtcttcctcg tcggcctgat cgcccccgac
     1081 cccccggccg gcctgcctct cgtcttccgg gcggcgcagg cgctcttcgt ccttctcttc
     1141 ctcttccgcg acgtccgcgg cgcgagcccc gggaaggcgc tcctggggct tgcggtcgtc
     1201 cggaccgacc gcggcccgct ccgggcgcgc gattcggtcc tccggaacct ccccctcctc
     1261 gttccgggcc tgaacctcct cgaggccctc tcggtcgtcc gccggcccga ttcccgccgc
     1321 ctcggcgacc ggctggccgg gaccgcggtc ggggaatcgt gaccggcgcg aaacttccgg
     1381 ccggtttcgt tcgtttctgc tggtggaggt cgccatggac cgcttcccga gactccctcg
     1441 ccacttcctc ccgctcgcgg ccctcgccgt ggcggccctc gccctgacgg gcggggcgct
     1501 cgccggccag gcgctcgagg agccgttcga gaagacctac gacgggaacg gcatcgagcg
     1561 cgtccggctc cagaacgtga acggtcccgt ccgggtcggc acatgggaga gagggcatgt
     1621 ccgcgtctcg gccgtgaagc gggcgaaggg ctcgcgcgcg gaggaggcga tcgcccagac
     1681 cgagatccgg gtgacgaagc gggccggagc gatcgagatc gaaacggtcc ttccgaagcc
     1741 gacgcggtcc tgggggttct tcagctgggg gaaccggggc ggagccgagg tcgcctacga
     1801 gctcctcctc ccggctgctc ttccggtcga cgtcgagacc gtcaacgggc gggtcacggc
     1861 cgagaagcgg ctcgggccgg tgacgctgaa cacggtgaac ggctccgtac gcgtcgagga
     1921 gcagggcggg ccgctgcacg tcaacacggt gaacggctcc gtcgaggtcg tcttctcggg
     1981 ctcgctccag acggccgacc tcgagaccgt gaacggctcc gtgaccgtca cctgcgcgcg
     2041 cgactcctcc ttccggttcg accttcagac ggtcaacggg aagatccggt ccgacttccc
     2101 cgacgtgacc gtcgagggga aatggggccc gaaggaagcc cgcggcgaga tcgccggggg
     2161 ccgtgagcgg ctgaccgtcg agacggtcaa cggcgaggtc cgcgtccttt ccggggacgc
     2221 ggcggcccgc tgagagggcg gattcccgta cactccggcc atcaccgaaa ggagatctcg
     2281 atgccggaag aagtcggagc tccggctccg ccgcccccgc ctcctccccc gtccgtcccg
     2341 ccggcgagct cgaaccggtc cgtaatgctc gtcctctcgt acctcgggat cctggccctg
     2401 atcccgctcc tcacggagaa gaacgaccgc gaggtgcagt ggcatgccaa gcacgggctc
     2461 gtgctgctcg tcgcctacct cgtcctgatg gtcggcatca tgatcgcgag cttcgtcgtc
     2521 ggcttcttcg gaatgctcca gatccccctc tggctcggct acctcgtcgt catcatcctc
     2581 gggatcacga acgccctgaa cggcaagcgg ttcgtcatcc ccggggtgtc ggagttcgcc
     2641 gagaagctct gagcggacgg gttcgcgaga ccccggaaac cgcggacgcc tcggaaagat
     2701 cgaaggcccg cctttcggcg ggccttcgcg ttcctgaaaa gtggtgccct gggccagact
     2761 cgaactggca cggattgctc cacacgcccc tcaaacgtgc gcgtctacca attccgccac
     2821 cagggcacgg tgggggaggg agatgatacc gatccgcgcc gtggaatcaa gcctcgaagc
     2881 ggatcgggcc gggattactt ctgcgccggc gcgggggccg gagcggcggg ggccgcgtct
     2941 gctgcggccg gggcggccgg caccgcgctc gccggtgccg gagcgacggg tacggcgccc
     3001 cccggagcgg gagcgacggg tacggcgttc gccggggcgg gggccgccgg tgcggccggg
     3061 gcgctcttga cgggctggct cccgagctgc ttgatgacgg acgacttcgg ccgggcctga
     3121 ataagcgaga tcgtcacggc gaggaccgag aacgcgacga acgaccaggt cgtcaccttc
     3181 tcgaggaccg tcgtcgcgcc gcgcgctccg aaggtcgtct gcgaggacga ggcgccgagc
     3241 gcgctcgcga cgtcggctcc cttcccctgc tggaggagga ccaccaggat gaggaagacg
     3301 cagacgatga cgtagatcgg gacgaggacg taaatcatgc gcgagtctcc ggcccgccgc
     3361 ccggaggcga aacgggccgg tgaggatacc ccaccggccc gaaaaggcca aaaccggccg
     3421 ggcggcgggc ccggggctca ggccccggcg gcgacggcga gagcggcgaa gtcggccggg
     3481 acgagcgagg cgcccccgac gaggccgccg tcgatgtcgg gcttcgcgaa cagatctgcc
     3541 gcattcgccg gggtcaccga cccgccatag aggatccgcg tcgcggccgc ggccgcgg
//
LOCUS       DAOTEZ010000437         3590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_19525_length_3590_cov_5.540594, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000437 DAOTEZ010000000
VERSION     DAOTEZ010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19525_length_3590_cov_5.540594"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="P5164_17390"
     CDS             complement(<1..235)
                     /locus_tag="P5164_17390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45708.1"
                     /translation="MKQETPEGEYVHVLVPSVVAIHRGKTWVGEGAKRLRARAGELGL
                     SPNRDLFWDTKNDIGVRKTYHRAPEGCRNASEIG"
     gene            653..1120
                     /locus_tag="P5164_17395"
     CDS             653..1120
                     /locus_tag="P5164_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014261374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRY45709.1"
                     /translation="MKRKTEPDRTEQRLERLRTAAREAGLKLTPQRLEVFREIAGTDE
                     HPDVETVFRAVQERMPTVSLDTVYRTLWALHDLGLVSTLGPQRNGVRFDANLERHHHF
                     SCVRCGLVRDFESPKLDDLPLPADLKGLGSVLNAHVEVRGLCTRCEAEKRKNA"
     gene            1173..3368
                     /gene="katG"
                     /locus_tag="P5164_17400"
     CDS             1173..3368
                     /gene="katG"
                     /locus_tag="P5164_17400"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HRY45710.1"
                     /translation="MSESGKCPFTGASGAPVAGRGTTNRDWWPDQLNLGILHQHDPAS
                     SPMDPGFRYAEEFGRLDYAALKKDLFALMTDSQEWWPADWGHYGGLFIRMSWHSAGTY
                     RVSDGRGGGGTGNQRFAPVGSWPDNGNLDKARRLLWPIKQKYGNRISWADLMILAGNC
                     ALESMGFRTFGFGGGREDVWQPEEEINWGREKTWLGDERYSGDRELASPLAAVQMGLI
                     YVNPEGPNGNPDPVASGRDVRETFARMAMNDEETVALVAGGHTFGKAHGAGDPKLVGP
                     EPEAAPLEQQGLGWKNALGTGKGAHTTTSGIEGAWKPNPTKWDMGYFDMLFGYEWELV
                     KSPAGAWQWLAKDVKPEHMIPDAHDPSKKHRPMMTTADLSLRFDPAYEKISRRFHADP
                     AAFADAFARAWFKLTHRDMGPRSRYLGPEVPAEVLIWQDPVPPVDHPLVDAADVAALK
                     AKVLASGLGVSELVATAWASASTFRGSDKRGGANGARVRLAPQKDWEANDPAQLAKVL
                     GVLEGIQREFDAAATGGKKVSLADLIVLAGGAAVEAAAKAAGCVVEVPFTPGRTDATP
                     EQTDAESFAVLEPKADGFRNYRKAGCASIEEHLLVDRAQLLGLGAPEMTVLVGGLRVL
                     GANSGGSKHGVFTERPGVLSNDFFVNLLDMGTVWKPTSEARETFEGSDRKTGAPRWTA
                     SRVDLVFGSNSQLRALAEVYAQDDAKEKLVRDFVAAFAKVMDLDRFDLA"
BASE COUNT          541 a         1280 c         1284 g          485 t
ORIGIN      
        1 cgccgatctc ggaggcgttc cggcacccct cgggggcgcg gtggtacgtc ttccggacgc
       61 cgatgtcgtt cttcgtgtcc cagaagaggt cgcggttcgg ggagaggccg agctcgccgg
      121 cgcgggcgcg aaggcgcttc gctccctcgc ccacccacgt cttcccgcgg tggatcgcga
      181 cgacggacgg gacgaggacg tggacgtact cgccctcggg cgtctcctgc ttcacctcga
      241 gggtcgtgat cgtccggccg ccgggcccga cgaccgcttc ggtgacggtg gagttggtgg
      301 tgccgaggtc gattccgagg accctcccgt gtcgtgcctc atcggaagtc cctccgccgg
      361 gaaggatcgg gccggaggga ggacagttgg tgtccctgga tgcgggggat gcgcgacggc
      421 gccgtcactt tcggcgcttc gcgaggcgcg cctcgaggaa ggcgatgtcc tcggcgtcgc
      481 ccggtgcaga gcggttctcc gaccctcccc cgcgcccgac ccctccggtg agacccctcc
      541 ggtgccaggc gtgaaatcgt gtattgttac aacaatacac gatttcacgc ctggcaccaa
      601 agcttcggtc gttggcgata gacttctggt tgcggatgat ccgcaactag gaatgaaacg
      661 caagaccgag cccgaccgga ccgagcagcg gctcgagcgc ctccggacgg cggcgcgaga
      721 ggcggggctg aagctgacgc cccagcgcct cgaggtcttc cgggagatcg cggggacgga
      781 cgagcacccc gacgtggaga ccgtcttccg cgccgtgcag gagcggatgc cgacggtctc
      841 gctcgacacg gtctaccgga ctctctgggc tctccacgac ctcggcctcg tctcgacgct
      901 cgggccccag cgaaacggcg tgcggttcga cgcgaacctg gagcggcacc atcacttctc
      961 gtgcgtccgc tgcgggctcg tgcgcgactt cgagagcccg aagctcgacg accttccgct
     1021 gccggccgac ctgaagggcc tcggcagcgt cctgaacgcg cacgtcgagg tgcgcggcct
     1081 ctgcacacgg tgcgaggccg agaagaggaa gaacgcgtag cccctgaccc gagcccggtc
     1141 ccgagcaacc cgccccacga aggagaacga cgatgagcga aagcggcaag tgcccgttca
     1201 ccggtgcttc cggcgccccc gtggccggcc ggggcacgac gaaccgagac tggtggcccg
     1261 accagctgaa cctcgggatc ctccaccagc acgacccggc ctcgagcccg atggaccccg
     1321 gcttccggta cgccgaggag ttcggcaggc tcgactacgc ggccctgaag aaggacctct
     1381 tcgccctgat gaccgactcg caggagtggt ggcccgccga ctggggccac tacggcggcc
     1441 tcttcatccg gatgtcgtgg cacagcgccg gcacgtaccg ggtctcggac gggcgcggcg
     1501 gcggcggcac cggcaaccag cgcttcgcgc cggtggggag ctggcccgac aacgggaacc
     1561 tcgacaaggc ccgccgcctc ctctggccga tcaagcagaa gtacgggaac cggatctcct
     1621 gggccgacct gatgatcctc gccggcaact gcgcgctcga gtcgatggga ttccggacgt
     1681 tcggcttcgg gggcggccgc gaggacgtct ggcagcccga ggaggaaatc aactggggcc
     1741 gggagaagac ctggctcggc gacgagcgct acagcggcga ccgcgagctc gccagcccgc
     1801 tcgcggcggt ccagatgggc ctcatctacg tgaaccccga agggcccaac ggcaaccccg
     1861 accccgtggc ctcggggcgc gacgtgcgcg agacgttcgc gcggatggcg atgaacgacg
     1921 aggagacggt cgcgctcgtc gccggcgggc acacattcgg gaaggcgcac ggcgcgggcg
     1981 acccgaagct cgtcgggccg gagccggagg cggccccgct cgagcagcag gggctcggct
     2041 ggaagaacgc gctcgggacg ggcaagggag cccacacgac gacgagcggc atcgaggggg
     2101 cctggaagcc gaacccgacg aagtgggaca tgggctactt cgacatgctc ttcggctacg
     2161 agtgggagct cgtgaagagc ccggccggcg cctggcagtg gctcgcgaag gacgtgaagc
     2221 ccgagcacat gatccccgac gcccacgacc cctcgaagaa gcaccggccc atgatgacga
     2281 cggccgacct ctcgctccgc ttcgacccgg cctacgagaa gatctcgcgc cgcttccacg
     2341 cggacccggc cgcgttcgcc gacgcgttcg cccgcgcctg gttcaagctg acgcaccgcg
     2401 acatggggcc gcgctcgcgc tacctcggcc cggaggtccc ggccgaggtt ctgatctggc
     2461 aggacccggt cccgccggtc gaccacccgc tcgtcgacgc ggccgacgtc gccgcgctga
     2521 aggcgaaggt cctcgcgtcg ggcctcggcg tctccgagct cgtcgcgacg gcctgggcct
     2581 cggcgtcgac gttccgcggc tccgacaagc gcggcggcgc caacggcgcc cgcgtgcgcc
     2641 tcgcgcctca gaaggactgg gaggcgaacg acccggcgca gctcgcgaag gtgctcggcg
     2701 tcctcgaggg gatccagcgc gagttcgacg ccgccgcgac gggcgggaag aaggtctcgc
     2761 tcgccgacct gatcgtcctc gccggcggag ccgccgtcga ggcggccgcg aaggcggccg
     2821 gctgcgtcgt ggaggtgccg ttcacgccgg gccgcaccga cgcgacgccc gagcagacgg
     2881 acgccgagtc gttcgccgtc ctcgagccga aggccgacgg gttccgcaac taccggaagg
     2941 ccggttgcgc ctccatcgag gagcacctgc tcgtcgacag ggcgcagctc ctcgggctcg
     3001 gcgcgccgga gatgacggtc ctcgtcggcg gcctgcgcgt cctcggggcg aactcgggcg
     3061 gttcgaagca cggagtcttc acggagcgcc cgggcgtcct gtcgaacgac ttcttcgtga
     3121 acctcctcga catggggacg gtctggaagc ccacgtccga ggcgcgggag acgttcgagg
     3181 ggagcgaccg gaagacgggc gcgccccgct ggaccgcgag ccgcgtcgac ctcgtcttcg
     3241 gctcgaactc gcagctgcgc gccctcgccg aggtctacgc gcaggacgac gcgaaggaga
     3301 agctcgtccg cgacttcgtg gcggccttcg cgaaggtgat ggacctcgac cgcttcgacc
     3361 tcgcctgatc gaggtccgga gatccccggc gggccgtcga gcccgctccc gccccggttc
     3421 ggcggccggc cagaggccgg tcgccgggcc ggggcgcttc tctcgtccct cgtccgcgga
     3481 cgctcgggag agccggcctg cgcggccgtc gtaccatcgg cggccatgcg tccccagcct
     3541 caccagagcg gccggtgcac ctcccggcgt tccctgcgcc ggcggctcgc
//
LOCUS       DAOTEZ010000438         3575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_19649_length_3575_cov_7.092614, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000438 DAOTEZ010000000
VERSION     DAOTEZ010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19649_length_3575_cov_7.092614"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1425
                     /locus_tag="P5164_17405"
     CDS             <1..1425
                     /locus_tag="P5164_17405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRY45711.1"
                     /translation="DDGLVHVTRDGGATWKNVTPKGLPEWTQVNSIEASPHDAGTAYL
                     AATLYKLGDLTPHLWKTADYGATWTRIDAGIARDAFTRVVREDPVRKGLLFAGTETGL
                     FVSLDDGKGWHRFQRNLPAVPITDLAVKDGDLVVATQGRAFWILDDLSPLRQWKDDVA
                     AAKLHLFAPPAAVRFPGGGDLAKPPKGAGQNAPAGALVSFWLKEAPKETDLVTLEVLD
                     GETVLRRFTNEKRDEKAATEAEEPLEKRLDLSAGLNRFSWDLRTLGPILVPKAVLWGS
                     KQGPLVAPGSYAVRLTAFGETKAAPLEVVPNPNVPFGKEELARQAAFLAELNETLSHI
                     HGTVRNLRDARAQVSALAERTKKIGVRPSVLEAAKELDARLLAVEEKLVNPRLKSGQD
                     VLNFVPALDHQVVGLVSAASSADAPPTAGALAYWAELKKTVAGALSDASRMLVDEVAA
                     WNARLADAGVPPVVVPVRRIPTLR"
     gene            complement(1447..1812)
                     /locus_tag="P5164_17410"
     CDS             complement(1447..1812)
                     /locus_tag="P5164_17410"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRY45712.1"
                     /translation="MHVEVRKKGDVVVADLTGRLVGGIGDEILREVVNELLAEGWKKI
                     LLNLSGVQVVDSAGIGELVASHKLCNKLGAQLRLLRLPKRVKETLHLAALLPVFHVFE
                     DEKDALHDLAAPAGPSAPG"
     gene            complement(1920..>3575)
                     /locus_tag="P5164_17415"
     CDS             complement(1920..>3575)
                     /locus_tag="P5164_17415"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRY45713.1"
                     /translation="ELPVVMASALGDTRNLVDALRLGANDYVIKPLDMQVVLARLGTQ
                     LALRRANEDVKRLASQLRAAQDQISTLLATGGEAFADFGRWARSMSSGVAGTLGAREI
                     SVFLLQEDAFVSVEGTRAEAPSPSAVALLPAGDGLSVEEERAVAPVRGPRGDLLGAVV
                     ADGKGEWDDVERGLLLGFAHQLGGALELQRMKEQLAAARQRSSRLGGATQSLEILYVC
                     PTCSRCWGPEVRACGADGTPLTEPRGFPHRVLDRYALRFLLGEGGMGAVFEAYDEKLD
                     RVVALKVVKPEHFRNATVRRRFVQEANAIAAIRHPNVVELHDSGDLGDGSMFLVMERL
                     HGRDLADLHRLSGAGTPVQVASLLRQGAAALGAAHRAGIVHRDVKPENVFLCPEDGGF
                     RVRILDFGLAKRVDGDRNLTQSGLIVGTPAWMPPEQISGREVDERADVYSFAAVCYLA
                     LTGRGVTEERDLDKVFVDVVRNPPPPVSALLPAAPAAVDAAFRAALAKKPEARPRDVV
                     AWATALAAALEGLPSSGAGWVFPAGGGPPGSPTAPTLVTRSPA"
BASE COUNT          517 a         1272 c         1329 g          457 t
ORIGIN      
        1 gacgacggcc tcgtccacgt cacgcgcgac ggcggggcga cctggaagaa cgtcacgccg
       61 aagggtctcc ccgagtggac ccaggtcaac tcgatcgagg cctcgccgca cgacgcgggg
      121 acggcttacc tcgcggcgac gctctacaag ctcggcgacc tgacccctca cctctggaag
      181 acggcggact acggggcgac ctggacccgg atcgacgccg gaatcgcgcg ggacgccttc
      241 acgcgcgtcg tgagggagga cccggtgcgg aaggggcttc tcttcgccgg gaccgagacg
      301 gggctcttcg tctcgctcga cgacgggaag gggtggcacc gcttccagcg gaacctcccc
      361 gccgtcccga tcaccgacct cgcggtgaag gacggagacc tcgtcgtcgc gacgcagggg
      421 cgggcgttct ggatcctgga cgacctctcg ccgctccggc agtggaagga cgacgtcgcc
      481 gcggcgaagc tccacctctt cgcgccgccc gccgccgtcc gcttccccgg cggcggcgac
      541 ctcgcgaagc cgccgaaggg agccgggcag aacgccccgg ccggcgcgct cgtctcgttc
      601 tggctgaagg aggcgccgaa ggagacggac ctcgtgacgc tcgaggtcct cgacggggag
      661 acggtcctcc ggcgcttcac gaacgagaag cgggacgaga aggccgcgac ggaggcggag
      721 gagccgctcg agaaacgact cgacctctcc gccggcctca accgattctc ctgggacctc
      781 cggacgctgg ggccgatcct cgtcccgaag gcggtcctct gggggtcgaa gcaggggccg
      841 ctcgtcgccc ccgggagcta cgccgtccgc ctcaccgcct tcggcgagac gaaggcggcg
      901 cccctcgagg tcgtcccgaa tccgaacgtg ccgttcggaa aggaggagct tgcgcggcag
      961 gccgcgttcc tcgccgagct gaacgaaacg ctctcgcaca tccacggcac cgtccggaac
     1021 ctgcgcgacg cacgcgcgca ggtctcggcc ctggcggagc ggacgaagaa gatcggagtc
     1081 cggccgtccg tgctcgaggc cgcgaaggag ctcgacgcgc ggctcctcgc cgtcgaggag
     1141 aagctcgtca atccgaggct gaagagcgga caggacgtcc tgaacttcgt cccggcgctc
     1201 gaccaccagg tcgtcggcct cgtcagcgcg gcctcgagcg ccgacgcccc gccgacggcg
     1261 ggcgccctcg cctactgggc cgagctgaag aagacggtgg ccggggcgct ctcggacgcg
     1321 agccggatgc tcgtcgacga ggtcgcggcg tggaacgccc ggctcgcgga tgccggcgtc
     1381 ccgccggtcg tcgtccccgt tcggcggatt ccgacgctgc gttgaggggt ccggaggcgg
     1441 cgccggtcag cccggggcgg agggtccggc cggagccgcc aggtcgtgca gggcgtcctt
     1501 ttcgtcctcg aagacgtgga agacggggag gagggccgcc aggtggagcg tctccttcac
     1561 cctcttcggg agccgcagga gccgcagctg ggcgccgagc ttgttgcaga gcttgtggct
     1621 cgcgacgagc tcgccgatcc cggcgctgtc gacgacctgg accccggaga ggttcagcag
     1681 gatcttcttc cacccctcgg cgaggagctc gttcacgacc tcgcggagga tctcgtcccc
     1741 gatccccccc acgagccgtc cggtcaggtc ggcgacgacg acgtcgccct tctttcggac
     1801 ctcgacgtgc atggtgggac gatcctagca gccctgccgg gaacagtgtg ctctacctcg
     1861 gacccggggg gtccgggggg ccgagaccag ccctccggcg aggcgagcag gctgccggat
     1921 caggcggggg agcgcgtgac gagggtcggc gccgtggggg agccgggggg gccgccaccc
     1981 gccgggaaaa cccagccggc tccgctcgag gggaggccct cgagcgctgc ggcaagcgcc
     2041 gtggcccagg cgacgacgtc gcgcgggcgg gcctcgggct tcttggcgag cgcggcccgg
     2101 aacgcggcgt cgaccgccgc gggcgcggcg gggaggagcg cggagacggg cgggggcggg
     2161 ttccgcacga cgtcgacgaa gactttgtcg aggtcccgct cctccgtgac gccgcgcccc
     2221 gtcagagcga ggtaacagac ggcggcgaag gagtagacgt cggccctctc gtccacctcc
     2281 cgcccggaga tctgctcggg cggcatccag gcgggggtcc cgacgatgag gccgctctgc
     2341 gtgaggttcc ggtcgccgtc gaccctcttg gcgaggccga agtcgaggat gcggacgcga
     2401 aagccgccgt cctccggaca gaggaagacg ttctccggct tgacgtcgcg gtggacgatc
     2461 ccggcccggt gggccgcgcc gagggcggcg gctccctgcc ggaggagcga ggcgacctgg
     2521 accggggtcc ccgcgcccga aaggcgatgg aggtccgcga ggtcccgccc gtggagccgc
     2581 tccatgacga ggaacatgga gccgtccccg aggtccccgg agtcgtggag ctcgacgacg
     2641 ttcgggtggc ggatcgcggc gatggcgttc gcctcctgga cgaaccggcg cctcaccgtg
     2701 gcgttccgga aatgctcggg cttgacgacc ttcagggcga cgaccctgtc cagcttctcg
     2761 tcgtaggcct cgaagaccgc gcccatccct ccttcgccca ggaggaaccg gagcgcgtag
     2821 cggtcgagga cacggtgcgg gaagccgcgg ggctcggtca gcggcgtgcc gtcggccccg
     2881 cacgcacgca cctccggccc ccagcagcgc gagcaggtcg ggcagacgta gaggatctcg
     2941 aggctctgcg tcgccccgcc gagccgggag gagcgctgcc gcgccgccgc cagctgctcc
     3001 ttcatccgct gcagctcgag cgccccgccg agctggtggg cgaagccgag gaggaggccg
     3061 cgctcgacgt cgtcccattc ccccttcccg tccgcgacga cggctccgag gaggtccccg
     3121 cgcggcccgc ggaccggcgc aacggccctc tcctcctcga cggagaggcc gtctcccgcc
     3181 ggcaggagcg cgaccgcgga gggcgagggc gcctcggccc gcgtcccttc cacggagacg
     3241 aacgcgtcct cctggagcag gaagacggag atctcccgcg ctccgagcgt tcccgcaacc
     3301 ccggacgaca tcgagcgggc ccagcggccg aagtcggcga aggcctcgcc gccggtggcg
     3361 agaagggtgg agatctggtc ctgcgcggcg cgcagctgcg acgccaggcg cttcacgtcc
     3421 tcgttcgccc gccggagcgc gagctgcgtg ccgagacgcg cgaggacgac ctgcatgtcg
     3481 agcggcttga tgacgtagtc gttcgccccg agccggagcg cgtcgacgag gttccgggtg
     3541 tccccgaggg cgctcgccat gacgacgggg agctc
//
LOCUS       DAOTEZ010000439         3565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_19743_length_3565_cov_4.898291, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000439 DAOTEZ010000000
VERSION     DAOTEZ010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19743_length_3565_cov_4.898291"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="P5164_17420"
     CDS             <1..858
                     /locus_tag="P5164_17420"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRY45714.1"
                     /translation="PAPPKGAPSITGDWGVEMGEGEKASRLLATFRQKGGEASGTLLG
                     PTGDYGPMSGTWDGGALVLSVFDGVFIYRIDAKADGPDGLSGTFRSRNGVPSRWTARR
                     LDAAGAAAWLPGGGSIVRAKEPGARVSFSYPSAPGKPVSLDDPEFANRPVLVTISGTW
                     CPNCHDEAPVLEELWRKYRGKGLAVVALSYEYTEDAERSFRQIARFRERHGLTYPVLF
                     AGTTKGAAASAPVSLLDGWKGYPTTLFLDRSHRIVKAHSGFDGPSTGARFSVQKKELE
                     TAVKEILAR"
     gene            complement(899..2374)
                     /gene="guaB"
                     /locus_tag="P5164_17425"
     CDS             complement(899..2374)
                     /gene="guaB"
                     /locus_tag="P5164_17425"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRY45715.1"
                     /translation="MPSSAPGIPLALTFDDVLLVPSRSEVHPSSVDLKTHLTADITLN
                     VPVISAAMDTVTEAPLAIAIAQNGGLGIVHKNLPIASQAAEVDKVKRSESGMIVDPVT
                     CRQDQKISEALDVMARFRISGVPVVDAAGKLAGILTNRDLRFETRMDLPISARMTKEN
                     LVTCAPGTTLEQAKQLLHEHRIEKLLVVDKAGNLKGLITVKDIQKQIKYPNACKDSLG
                     RLRVGAAVGAGADLVDRARELIAAKVDVLCLDSSHGHSEGVLQALKLLKKTFPSIPVI
                     AGNVGTAEATRDLIRLGADAVKIGIGPGSICTTRVVTGVGVPQIHAILECVKGRGKSR
                     VPLIADGGIKFSGDVTKALAFGAQSVMIGSLFAGTDESPGETILYQGRTFKAYRGMGS
                     LGAMTKGSADRYFQNSGDEPRKMVPEGIEGMVPHKGSLSVLLAQIAGGVRAGMGLSGA
                     RTLPELRKKAKFVRITSAGLKESHVHDVIITKEAPNYRQEP"
     gene            complement(2451..>3565)
                     /locus_tag="P5164_17430"
     CDS             complement(2451..>3565)
                     /locus_tag="P5164_17430"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HRY45716.1"
                     /translation="DNQLRGRAGRQGDPGSSRFYLSLEDDLMRIYGSDRLSGLMKRLG
                     MEEGVPIEHRWVTKSIERAQKQIEARNFDTRKHLLQYDDVMNKQREEIYRLRKEILTG
                     ALSHEYVVNLAEDVAEDFVDRHCPAGKDHSEWDWKALTSVLGESFGMSPEEAGLSQES
                     DNNAALKDKLKAALRSTYEEKTARLGADVMKDFEKFFMLQTVDALWKDHLLALDHLKE
                     GIGLRGYGQRDPLVEYKKESFQLFEAMKEAIEEQILQNLFRFEVVRPEEAEARWASSA
                     PAASAPPAPSEEGAAPSGGVSAPALSTRASAELERRAQRQRREMSFQGTSDPTSGGDF
                     RVDTVKAAGPKVGRNDPCPCGSGKKYKKCHGATSAA"
BASE COUNT          484 a         1197 c         1296 g          588 t
ORIGIN      
        1 ccggcgccgc cgaagggagc gccctcgatc acgggcgact ggggcgtcga gatgggagaa
       61 ggggagaagg cttcccgcct cctcgcgacg ttccgccaga aggggggcga ggcgtccggc
      121 acgctgctcg ggccgaccgg cgactacggc ccgatgagcg ggacctggga cgggggggcg
      181 ctggtcctct cggtctttga cggtgtcttc atctaccgga tcgacgcgaa ggccgacggt
      241 cccgatgggc tctcgggcac gttccggagc cggaacgggg tcccgtcgcg ctggacggcc
      301 cgccgcctcg acgcggccgg agccgcggcc tggctcccgg ggggcggctc gatcgtccgc
      361 gcgaaggagc cgggggcgcg ggtctcgttc tcgtatccct cggcgccggg gaagccggtc
      421 tcgctcgacg acccggagtt cgcgaacagg ccggtcctcg tcacgatctc ggggacctgg
      481 tgcccgaact gccacgacga ggcgcccgtc ctcgaggagc tctggaggaa gtaccgcggc
      541 aaggggctcg cggtcgtcgc gctctcctac gagtacaccg aagacgccga gaggagcttc
      601 cggcagatcg cccggtttcg cgagcgccac ggcctgacct atccggtcct cttcgccgga
      661 acgacgaagg gggcggcggc ctcggcgccg gtttccctgc tggacggttg gaagggctac
      721 ccgacgacgc tcttcctcga ccggagccac cggatcgtga aggcgcactc cggcttcgac
      781 ggaccgtcca ccggcgcccg gttctcggtg cagaagaagg agctggagac ggcggtgaag
      841 gagatcctgg cccgctagcc ggtcggcctc cgggcgcgac gcgcccggag gctccgcgtc
      901 agggctcctg gcggtagttc ggggcctcct tcgtgatgat gacgtcgtgg acgtggctct
      961 ccttgagccc ggccgacgtg atccgcacga acttcgcctt cttccggagc tcggggagcg
     1021 tccgcgcgcc cgagaggccc atgcctgccc gcacgccgcc ggcgatctgc gcgaggagga
     1081 ccgagaggga gcccttgtgg ggcaccattc cttcgatccc ctcggggacc atcttgcggg
     1141 gctcgtcccc ggagttctgg aagtagcggt ccgccgaccc cttcgtcatc gcgcccagcg
     1201 agcccattcc gcggtacgcc ttgaacgtgc gtccctggta gaggatggtc tctcccggcg
     1261 actcgtcggt gccggcgaag aggctcccga tcatgacgga ctgcgcgccg aaggcgagcg
     1321 ccttcgtgac gtcgcccgag aacttgatcc cgccgtcggc gatgaggggg accctcgact
     1381 tcccgcgtcc cttcacgcac tcgaggatcg cgtggatctg cgggacgccg acgccggtga
     1441 cgacgcgggt cgtgcagatc gagcccggcc cgatcccgat tttcaccgcg tcggccccga
     1501 ggcggatcag gtcgcgcgtc gcctcggcgg tgccgacgtt gccggcgatg acggggatcg
     1561 acgggaaggt cttcttcagg agcttcagcg cctggaggac cccctcgctg tggccgtgcg
     1621 aggagtcgag gcagaggacg tcgaccttcg cggcgatgag ctcgcgcgcc cggtcgacga
     1681 ggtcggcgcc cgcgccgacc gccgcgccga cgcggaggcg gccgagcgag tccttgcagg
     1741 cgttcgggta cttgatctgc ttctggatgt ccttgacggt gatcagcccc ttgaggttgc
     1801 ccgccttgtc gacgacgagg agcttctcga tccggtgctc gtgaaggagc tgcttggcct
     1861 gctcgagcgt cgtcccgggc gcgcaggtga cgaggttctc cttcgtcatc cgggccgaga
     1921 tcggaaggtc catccgggtc tcgaaccgga ggtcccggtt cgtcaggatc cccgcgagct
     1981 tgcccgccgc gtcgacgacc gggacgccgg agatccggaa tcgggccatc acgtcgagcg
     2041 cctcggagat cttctggtcc tggcggcagg tgacggggtc gacgatcatc ccgctctccg
     2101 agcgcttcac cttgtcgacc tcggccgcct ggctcgcgat ggggaggttc ttgtggacga
     2161 tcccgaggcc gccgttctgg gcgatggcga tcgcgagcgg cgcctcggtg accgtgtcca
     2221 tcgcggcgga gatcaccggg acgttcaggg tgatgtcggc cgtgagatgg gtcttcaggt
     2281 cgaccgacga gggatggacc tccgagcgcg acgggacgag gaggacgtcg tcgaacgtga
     2341 gggcgagggg gatgccggga gcggaagacg gcatgccgat tcatagcacg tccggggccg
     2401 ctccgctggg agcggccccg gacggggcga aacgggcgtc gagaaggggg tcaggcggcc
     2461 gacgtggccc cgtggcactt cttgtacttc ttgccggagc cgcaggggca ggggtcgttc
     2521 cggcccacct tcgggccggc cgccttcacg gtgtcgaccc ggaagtcgcc gccggacgtc
     2581 gggtcggacg tcccctggaa ggacatctcc ctccgctgcc tctgggctct ccgctcgagc
     2641 tccgcggagg cgcgcgtcga gagcgccggc gccgagaccc cgcccgaggg ggccgcccct
     2701 tcttccgagg gggccggggg ggcggacgcg gcgggcgcgg aggaagccca gcgggcctcg
     2761 gcttcctccg gcctcacgac ctcgaagcgg aagaggttct ggaggatctg ctcctcgatc
     2821 gcctccttca tcgcctcgaa gagctggaac gactccttct tgtactcgac gagggggtcg
     2881 cgctggccgt agccgcgcag gccgatcccc tccttcaggt ggtcgagggc gaggagatgg
     2941 tccttccaca gggcgtcgac ggtctggagc atgaagaact tctcgaagtc cttcatgacg
     3001 tcggcgccga gccgggccgt cttctcctcg taggtgctcc ggagagccgc cttgagcttg
     3061 tccttcaggg ccgcgttgtt gtccgactcc tgcgagagcc cggcctcctc gggcgacatc
     3121 ccgaaggact ccccgaggac cgacgtgagg gccttccagt cccactcgga gtggtccttc
     3181 ccggcggggc agtgccggtc gacgaagtcc tcggcgacgt cctcggcgag gttcacgacg
     3241 tactcgtgcg acagcgcgcc cgtgaggatc tccttccgga gccggtagat ctcctcgcgc
     3301 tgcttgttca tgacgtcgtc gtactgaagg aggtgcttgc gcgtgtcgaa gttccgcgcc
     3361 tcgatctgct tctgggcccg ctcgatcgac ttcgtcaccc agcggtgctc gatcgggacc
     3421 ccctcctcca ttccgagccg cttcatgagg cccgagaggc ggtcggaccc gtagatccgc
     3481 atcaggtcgt cctcgagcga caggtagaag cgcgaggagc cggggtcgcc ctggcggccg
     3541 gcgcggccgc ggagctggtt gtcga
//
LOCUS       DAOTEZ010000440         3563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_19759_length_3563_cov_5.495154, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000440 DAOTEZ010000000
VERSION     DAOTEZ010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19759_length_3563_cov_5.495154"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..1349
                     /locus_tag="P5164_17435"
     CDS             21..1349
                     /locus_tag="P5164_17435"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRY45717.1"
                     /translation="MNTSRNAVVIVGGQWGDEGKGKVVDLISPAFDVVARYQGGHNAG
                     HTVKFEDRHFALRLIPSGICRKGVLNVIGNGLVVDPRALVAEIASLRAAGVPVGENLK
                     LSDRAHVILPTHALLDGARENALGGANIGTTSRGIGPAYESKANRSGIRVADLVDPDR
                     FVSLARPLLSHHAAVLSHFYGVEAPSVDETIDTYLECGRDLAAHVADTRALLHERLAR
                     GARLLCEGAQGVMLDVDHGTYPFVTSSSCGPGGACTGLGIPPSAIGAVVGIMKAYTTR
                     VGSGPFPTELFDQTGERIRNRGNEFGTVTGRPRRVGWFDAVVARTSLKVGGLDAIALT
                     KMDVLDEHDEVRICVAYEIGGRTVTEVPARSDHYEAARPVYRTFPGWNRSIVGLDDVA
                     KLPEAARAYVAALEETVGAPITLLSTGPKREETIIRPGTVLDTWLSAARR"
     gene            1378..1962
                     /locus_tag="P5164_17440"
     CDS             1378..1962
                     /locus_tag="P5164_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006893926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRY45718.1"
                     /translation="MAIAGLLLAAGSSRRFGGAKLLAPLPDGTPVGVRSARTLAGAVE
                     RALAVVRPDDTVLARLLAEQGLEVRAFAGAAEGMGASLAFGVAATPEADGWLVALADM
                     PWVSAETVRAVADLLRDGALIAAPCRTGRRGHPVGFGYSLISELIGLRGDRGAREILS
                     RHADRITLLEVDDDGILLDIDELSDLQQSITRMA"
     gene            2139..>3563
                     /locus_tag="P5164_17445"
     CDS             2139..>3563
                     /locus_tag="P5164_17445"
                     /inference="COORDINATES: protein motif:HMM:NF019307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HRY45719.1"
                     /translation="MASTLDARIAELAAKYLPLAKEILSEAIRIPADYVDKPLEKGGD
                     PLCGTSNHEFPRLEYLRKKIVEIQAVRKPEDVFFDGFGNLVWQVEDPNDGIPSAQKKV
                     VYIDGHTDTVKPLRAQWKERVVGADCFDGLVDASKLNKEFLRKELGWTPPESEWEHLL
                     FGRGSADQLGGVISAIIATKILVELTDEGALRGAIVRSYGTACEEDNDGAGPMYLNRE
                     VFPTAGAEVIPDVVILTDGTGCSKRGALGIYRGQRGRMQIEVTVTGKSCHGSMPWEGK
                     NPLEYGGAIVKEAAEAYEKRDGFLDDPFLGHGTRTASWARLDTPSDCAVPEKLVFRFD
                     RRLTIGETPEQAVADVERLSSVAAARKAGLSVEVSIPTYTDASWKGYVLNNPQVYLGW
                     VTPEEHPSIRAAVETYRGVVSPHVDGKIGEGGVLTKEPRVDRWVFSTDGVGFPVPKDA
                     KIPGAAGKGWIESGPYKYPAMLGFG"
BASE COUNT          558 a         1291 c         1200 g          514 t
ORIGIN      
        1 ccggcgggta gaatcgacag gtgaacacgt ctcgcaacgc cgtcgtgatc gtcggtggac
       61 agtggggcga cgaggggaag gggaaggtcg tcgacctgat ctccccggcc ttcgacgtcg
      121 tcgcgcgcta ccagggcggc cacaacgccg gccacaccgt caagttcgag gaccgccact
      181 tcgccctccg gctcattccc tccggcatct gccggaaggg cgtgctgaac gtcatcggga
      241 acggcctcgt cgtcgacccg agggcgctcg tcgccgagat cgcgagcctt cgcgccgccg
      301 gcgtcccggt cggcgagaac ctgaagcttt ccgaccgggc tcacgtgatc ctcccgacgc
      361 acgcgctcct cgacggcgcg cgggagaacg cgctcggcgg ggcgaacatc gggacgacct
      421 cgcgcggtat cggcccggct tacgagtcga aggcgaaccg gagcgggatc cgggtggcgg
      481 acctcgtcga ccccgaccgg ttcgtctcgc tggcccgccc gctcctgagc catcacgcgg
      541 ccgtcctctc gcatttttac ggcgtcgagg cgccctcggt cgacgagacg atcgacacct
      601 atctcgagtg cggacgcgac ctcgcggcgc acgtcgccga cacgcgggcg ctcctgcacg
      661 agaggctcgc tcgcggggcc cggctcctct gcgagggggc ccagggcgtg atgctcgacg
      721 tcgaccacgg gacctacccg ttcgtcacgt cctcctcctg cggcccgggc ggggcctgca
      781 ccggcctcgg gatccccccc tccgcgatcg gcgccgtcgt ggggatcatg aaggcctaca
      841 cgacccgcgt cgggtccggg cccttcccga ccgagctctt cgaccagacc ggcgagcgga
      901 tccggaaccg cgggaacgag ttcggcaccg tgaccgggcg gccccggcgc gtcggctggt
      961 tcgacgcggt ggtggcgcgg acgagcctga aggtcggcgg cctcgacgcc atcgccctca
     1021 cgaagatgga cgtcctcgac gagcacgacg aggtccggat ctgcgtggcc tacgagatcg
     1081 gcggccggac ggtgaccgag gtcccggccc gctccgacca ctacgaggcg gcccggccgg
     1141 tttatcgcac cttccccggc tggaatcgct ccatcgtcgg tctcgacgac gtcgcgaagc
     1201 tccccgaggc ggcccgggcc tacgtcgccg ccctcgagga aaccgtcggc gccccgatca
     1261 ccctgctgtc gacgggcccg aagcgcgagg agacgatcat ccgccccggg accgtcctcg
     1321 acacctggct ctccgccgcg cggcgctgag cttcgacgga ttcggcggga ggaggacgtg
     1381 gccatcgcgg gcctcctcct cgccgcgggg agctcccgcc gcttcggcgg cgccaagctc
     1441 ctcgccccac ttcccgacgg cactccggtc ggcgtccggt cggcgcgcac gctggccggc
     1501 gctgtcgagc gcgccctcgc ggtcgtccgg cccgatgaca ccgtgctcgc ccgcctcctc
     1561 gccgagcaag ggctcgaggt gcgagcgttc gcgggcgccg ccgaggggat gggggcgagc
     1621 ctcgcgttcg gcgtcgctgc gacgccggag gcggacggct ggctcgtcgc cctcgccgat
     1681 atgccgtggg tgagcgccga gacggtccgc gccgtcgcgg atcttcttcg ggacggggcc
     1741 ctgatcgcag cgccgtgccg cacgggtcgt cggggtcacc ccgtcgggtt cggatacagt
     1801 ctcatttcgg agctcattgg gcttcgaggc gaccgcggag cgcgcgagat cctttcccgc
     1861 catgccgatc ggatcacgct cctggaagtg gacgacgacg gtatcctact ggatatagat
     1921 gaattaagtg atcttcaaca atcgataaca cgtatggcgt aaaaattttc gctttagaca
     1981 aaaattttct tgactctcgc ccccggcggc ccgatattga tgcgggaaga cggaatggac
     2041 tcgaaccccg agaccccgac ccgcagcgaa ccaggcaaca ccgcctgtct cgacaccccg
     2101 ccggaggaca agatgccgac ctcacttcgc ccgtcgccgt ggcttccacc ctcgacgccc
     2161 gcatcgccga gctcgccgcg aagtacctcc cgctcgccaa ggagatcctc tcggaggcga
     2221 tccgaatccc ggccgactac gtcgacaagc cgctcgagaa gggcggcgac ccgctctgcg
     2281 gcacttccaa ccacgagttc ccgcgcctcg agtacctccg gaagaagatc gtcgagatcc
     2341 aggcggtgag gaagcccgag gacgtcttct tcgacggctt cggcaacctc gtctggcagg
     2401 tcgaggaccc gaacgacggc atcccctccg cgcagaagaa ggtcgtctac atcgacggcc
     2461 acaccgacac cgtcaagccc ctccgggcac agtggaagga gagggtcgtc ggcgccgact
     2521 gcttcgacgg cctcgtcgac gcgtcgaagc tcaacaagga gttcctgagg aaggagctcg
     2581 gctggacgcc gcccgagtcc gagtgggagc acctcctctt cggccgcggc tcggccgacc
     2641 agctcggcgg cgtcatctcc gcgatcatcg cgacgaagat cctcgtggag ctcacggacg
     2701 aaggggcgct tcgcggcgcg atcgtccgct cctacggcac ggcctgcgag gaggacaacg
     2761 acggcgccgg tccgatgtac ctgaaccgcg aggtcttccc gacggcgggc gccgaggtga
     2821 tccccgacgt cgtcatcctc acggacggca ccggctgcag caagcgcggc gccctcggca
     2881 tctaccgcgg ccagcgcggg cggatgcaga tcgaggtcac cgtcacgggc aagtcgtgcc
     2941 acggctctat gccctgggag gggaagaacc cgctcgagta cggaggcgca atcgtcaagg
     3001 aggccgccga ggcctacgag aagcgcgacg gcttcctcga cgacccgttc ctcggccacg
     3061 gcacccggac cgcctcgtgg gccaggctcg acacgccgag cgactgcgcc gtccccgaga
     3121 agctcgtctt ccgcttcgac cgccgcctga cgatcggcga gacgcccgag caggcggtcg
     3181 ccgacgtcga gcgcctctcc tcggtcgccg ccgcccgcaa ggccggcctc tccgtcgagg
     3241 tctccatccc gacctacacc gacgcgagct ggaagggcta cgtcctgaac aacccgcagg
     3301 tctacctcgg ctgggtcacg ccggaagagc acccttcgat ccgggcggcg gtggagacgt
     3361 acaggggcgt cgtcagcccg cacgtcgacg ggaagatcgg cgaggggggc gtcctgacga
     3421 aggagccgcg cgtcgaccgc tgggtcttct cgacggacgg cgtcggcttc ccggtcccga
     3481 aggacgcgaa gatcccgggc gccgcgggaa agggctggat cgagagcggc ccctacaagt
     3541 accccgcgat gctcggcttc ggc
//
LOCUS       DAOTEZ010000441         3559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_19794_length_3559_cov_6.837043, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000441 DAOTEZ010000000
VERSION     DAOTEZ010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19794_length_3559_cov_6.837043"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..618
                     /locus_tag="P5164_17450"
     CDS             115..618
                     /locus_tag="P5164_17450"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HRY45720.1"
                     /translation="MTNRVRSSTRREAGKAVLFGALLLAACSGGRGAPPAAPAPAAGP
                     RVVLPSGAVFALELARTPEQIAQGLMFRESLAPSSGMVFLFDAPEPRSFWMKNCHFPL
                     DMVFTLADGAVVDVLENVPPCEADPCPSYPSRAPADTVVELTGGEAKRHGVVPGARLS
                     FLSVPGR"
     gene            complement(599..2446)
                     /locus_tag="P5164_17455"
     CDS             complement(599..2446)
                     /locus_tag="P5164_17455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45721.1"
                     /translation="MRSRRADLAAALLAVVLAPALDASDGRPVTAPGGTWLRYPLPGA
                     EVKSLVAEPRSPGLFYAGTALGGVYRSTDGGRTWSAPPGGAPFPGYSVTSLLPDPQRV
                     GTVWAGLTGVVKGGLLVRSDDAARTWSVVRRWEERAEARVVAVAVLRGRKVVAVGGDT
                     GLEISEDGGLTWRPSQPPLDPGSGVAFLAFHPLESGVLYSGSYRHPFRSTDLGRSWKR
                     IAGGMVEDTQVFHLDFSPTDPNDIWAATCGWVYRTTDGAATWRRYKDGLADRRTHVVA
                     RDPRDPDRVLAGTTGGLFESPDAGKSFRRISRESTVVNALVFDPANPKSLLVATEAEG
                     ILRSEDGGLTLLESNAGLSEARVSAVAVTASGRVAVARAADGGSGGLWDLDPVSGKAE
                     RIASAPAATIRALLASGERLLAGTPDGVWLAVEPGAPFGRVLGLPVHGFAAGPGRLFA
                     ATDGGAFESRDGGSTWGRVGDLRTRVETVMWSRSPGTGAETLAVRSSGRTLWWNGRDF
                     GLDARSGANPRPLSGGFGRPRASAYRPAEPIGLDVDSEKGRLLYRVPGPGADVALALP
                     ERGLHVAGWAGDPLSARGLYLATIGRGLFRFVPFAAGAAAAGELSAPAR"
     gene            complement(2427..2786)
                     /locus_tag="P5164_17460"
     CDS             complement(2427..2786)
                     /locus_tag="P5164_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRY45722.1"
                     /translation="MPLYEFRCESCGHTMETILAFSDPLPTACESCGGALKKLLSAPA
                     VQFKGSGFYLTDYGRSGAAGPKGRDSGGDAEPKPAGESKPTGESKASAETKAGGDAKT
                     TAAASGSGTGGDAKPAG"
     gene            complement(2806..>3559)
                     /locus_tag="P5164_17465"
     CDS             complement(2806..>3559)
                     /locus_tag="P5164_17465"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HRY45723.1"
                     /translation="PEVLQTVHHYRVPGVVVVRREAVEKKVYIWNGDVIFATSNDREE
                     SLGNVLLADGKLTREQFDESVRRLVEARSTEGARRHGAVLVDMGLLTAEELFAAVTRQ
                     VRGILFSLFQWDEGEVTFTVGRFRTDEIIQLEIPTRQAILAGVKDLRDARKTVSLLGP
                     SWTIFDPSWKPAELGDLALEPGELRLLQQVDGVKTLRELVGLGPSDAAHNAKLLYAFY
                     VLKLIGRRDMTSRTAVKKIQWSTTGGGYAPPS"
BASE COUNT          480 a         1324 c         1297 g          458 t
ORIGIN      
        1 agcgcgcgaa cggagtccgg aaggaagccg cgaagctcgt cgggatgtcg gagcgttcgt
       61 tccgctacta cgccaagaag tacaacctcg gccacgacga cgaaggagag ccttatgacg
      121 aaccgggtcc ggtcgtcgac gaggcgtgaa gccgggaagg ccgtcctctt cggcgccctc
      181 cttctcgcgg cctgctccgg gggacgcggc gcgccgcccg ccgcaccggc cccggccgcc
      241 gggccgcgag tcgtcctccc gtcgggcgcc gtcttcgccc tcgagctcgc gcggacgccg
      301 gagcagatcg cacaggggct catgttccgc gagtcgctcg cgccgtcgag cgggatggtc
      361 ttccttttcg acgcgcccga gccgcggtcg ttctggatga agaactgtca cttcccgctc
      421 gacatggtct tcacgctcgc ggacggggcc gtcgtcgacg tcctggagaa cgtcccgccg
      481 tgcgaggccg acccgtgccc gagctatccg tcgcgcgcgc ccgccgacac ggtggtcgag
      541 ctgacgggcg gagaggcgaa gcgccacggg gtcgtcccgg gcgcacgcct ctcgttcctc
      601 agcgtgccgg ggcgctgagc tccccggcgg cggcggcgcc cgcggcgaac gggacgaagc
      661 ggaagagccc ccgcccgatc gtcgcgaggt agaggccccg cgcgctgagc gggtcccccg
      721 cccagccggc gacgtggagg ccccgctccg gaagcgccag ggcgacgtcg gcgccgggcc
      781 ccgggacgcg gtagaggagg cgccccttct cggagtcgac gtcgagcccg atcggctcgg
      841 ccggccggta ggccgaggcg cgcgggcggc cgaaccctcc cgaaagcggg cgcgggttcg
      901 cgccgctccg cgcgtcgagg ccgaagtccc gtccgttcca ccagagggtc cggcccgagg
      961 agcgcacggc gagcgtctcg gccccggtcc ccggcgagcg gctccacatc acggtttcca
     1021 cgcgcgtccg gagatcgccg acgcggcccc aggtcgaccc cccgtcgcgc gactcgaacg
     1081 caccgccgtc ggtcgcggcg aagagacgtc ccggtccggc cgcgaagccg tggaccggaa
     1141 ggccgaggac cctcccgaac ggcgcgcccg gctccacggc gagccagacc ccgtccggcg
     1201 tcccggcgag cagccgctcg ccgctcgcga ggagggcgcg gatcgtcgcg gcgggcgcag
     1261 acgcgatccg ctcggccttc ccggacacgg ggtcgaggtc ccacagcccg ccgctcccgc
     1321 catccgcggc ccgcgcgacg gcgacccgcc ccgacgcggt gacggcgacg gccgagaccc
     1381 gcgcctccga gaggcccgcg ttcgactcga ggagcgtcaa cccgccgtcc tcgctccgga
     1441 ggatcccctc cgcttcggtc gcgacgagga gcgatttcgg gttcgccggg tcgaacacga
     1501 gcgcgttgac gacggtcgac tcccgcgaga tgcgccggaa gctcttcccc gcgtccgggc
     1561 tctcgaagag cccgccggtc gtcccggcga ggacgcggtc cgggtcccgc gggtcgcggg
     1621 cgacgacgtg ggtccggcgg tcggcgaggc cgtccttgta ccggcgccag gtcgccgcgc
     1681 cgtccgtcgt ccggtagacc cagccgcagg tcgccgccca gatgtcgttc gggtcggtcg
     1741 gcgagaagtc gaggtggaag acctgcgtgt cctcgaccat cccccccgcg atccgcttcc
     1801 agctccggcc caggtccgtc gagcggaaag gatgccggta cgagcccgag tagaggacgc
     1861 cgctctcgag cgggtggaac gcgaggaacg cgacgccgct tcccggatcg agcgggggct
     1921 gggagggccg ccacgtgagg ccgccgtcct cggagatctc gagacccgtg tcgcctccga
     1981 ccgcgacgac cttccgtccg cgcaggacgg cgaccgccac gacgcgggcc tcggcccgct
     2041 cctcccaccg tcgcacgacg ctccaggtgc gggcggcgtc gtccgatcgg accaggagcc
     2101 cccccttgac gacgcccgtc agaccggccc agaccgtccc gacgcgttgc gggtcgggga
     2161 gcagagaggt gacggaatag ccggggaacg gggcgccgcc cggaggagcg ctccaggtcc
     2221 ggccgccgtc ggtcgatcgg tagacgccgc cgagggcggt cccggcgtag aagaggcccg
     2281 gcgagcgcgg ctccgccacg agcgacttca cctcggctcc cggaaggggg tagcgcagcc
     2341 acgtccctcc gggggccgtg accggccgcc cgtcggaggc gtcgagagcg ggcgcgagga
     2401 cgaccgcgag gagcgcggcc gcgaggtcag cccgccggct tcgcatcgcc ccccgtgccg
     2461 gaaccgctcg ccgccgcggt ggtcttcgcg tcgccccccg ccttcgtctc ggcggaggcc
     2521 ttcgactcgc cggtcggctt cgactctccc gcgggcttcg gctccgcgtc gccgccggag
     2581 tccctcccct tcggcccggc ggcgcccgac cggccgtagt cggtgaggta gaagccggag
     2641 cccttgaact ggacggcggg agccgagagg agcttcttca gcgcgccgcc gcacgactcg
     2701 caggcggtgg ggaggggatc ggagaacgcg aggatcgtct ccatggtgtg accgcacgac
     2761 tcgcaccgga actcgtagag gggcatctcg aaacggacct ttctctcacg acggcggggc
     2821 gtagccgccg cccgtcgtgc tccactggat cttcttcacg gccgtccggc tcgtcatgtc
     2881 gcgccggccg atcagcttga ggacgtagaa ggcgtacagc agcttcgcgt tgtgcgccgc
     2941 gtcggagggc ccgagcccga cgagctcgcg gagggtcttc acgccgtcga cctgctggag
     3001 gaggcggagc tctcccggct cgagcgcgag gtcgccgagc tccgccggct tccacgacgg
     3061 atcgaagatc gtccaggagg ggccgaggag cgagacggtc ttccgggcgt cgcgcaggtc
     3121 cttcacgccg gcgaggatcg cctgccgcgt cgggatctcg agctggatga tctcgtcggt
     3181 ccggaagcgg cccaccgtga aggtgacctc cccctcgtcc cactggaaga gcgagaagag
     3241 gatcccgcgg acctgccgcg tcaccgcggc gaagagctcc tcggccgtca ggaggcccat
     3301 gtcgacgagg acggcgccgt gccgccgggc cccttcggtc gacctcgcct cgacgagccg
     3361 gcggaccgac tcgtcgaact gctcgcgcgt cagcttcccg tctgcgagca ggacgttccc
     3421 gagggactcc tcccggtcgt tcgaggtcgc gaagatgacg tcgccgttcc agatgtagac
     3481 cttcttctcg accgcttcgc ggcggacgac gaccacgccg ggcacccggt agtggtgcac
     3541 cgtctggagg acctcggga
//
LOCUS       DAOTEZ010000442         3557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_19814_length_3557_cov_6.159623, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000442 DAOTEZ010000000
VERSION     DAOTEZ010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19814_length_3557_cov_6.159623"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="P5164_17470"
     CDS             <1..719
                     /locus_tag="P5164_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45724.1"
                     /translation="LKVAAVEEGPSEVVFVVAGEALDDLRRLMPYSSRSQVANAAAER
                     IDLPRGTRYRFLGTVPKTFDSPTEVVSLFAASEDFTPRDGSFFRVVGRLAIAKRPEDP
                     RVAESVAVAGLSAAARSRRRAVVLLLGTGAAEEGLVEAPQTRRFLSRLGVPLHVWDVS
                     RPSAGAAPDLPPPAVERADARPHWPEAVRITDLSSLLSAFRGVEKELSSQRIVWLHGR
                     LNPAKVELTAAAGKEVALVP"
     gene            716..1771
                     /locus_tag="P5164_17475"
     CDS             716..1771
                     /locus_tag="P5164_17475"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRY45725.1"
                     /translation="MRAASRSLAVLFAGLLLLAARSAAAEHVTLPAGARLHLEPDPSS
                     PALTMLDEETLVEVLERKDDWVRVGYGTWRGWAREAAPVSLLSERGGPNVPQEWVAGT
                     LLARARSLLRDGGREVPFARRALVTDVRDDALLARLEEAVLAATASLGDRWKLRVPVK
                     SGSYVFLFESVEAERKLGDVACGPIGPAGSRVVSLALAGDGAAPTLERLLHEVGHLYA
                     RELLGDGVPNWVEEGMADAFVEGGTSPSRSRWSRRPLPVRPASSLPPPSVAGLIRAGP
                     ELFERGPAGEALRAETISFFRYLSDPWLPRFKPFRSYLRRGSGGKPLDQPALEKALGE
                     SLAGLERTWRAYRGRGE"
     gene            1846..3132
                     /locus_tag="P5164_17480"
     CDS             1846..3132
                     /locus_tag="P5164_17480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45726.1"
                     /translation="MIAFLTLFLGIVVGPHPVELSAEPGVVRVELSLDGALVASLGPP
                     WATTLDLGREIAPRELVAVAYDAAGKRLGEASQWINRARPEAEAGFALKRDGSGRVSA
                     ARLFWRCPVSPDPESIAVTLDGVPLEAKDPARIRIPSRSPDTAHILIADLTFPGGIVA
                     TAVTTFDGRTRKESVRELSAVPIRILPGARLPKQVDGMRGWFESNGQALSVVAVEEGQ
                     AEVVFVQAGTIGPDFERLLVESFQAPPGARPVDLRKDSRFLFLSSNPRASVAPNAVTR
                     LFPISRDYPSRGGGFLEVGATPFAREPRTPPRIAEAVATAGLSATRRERRRAVVLLLG
                     AGARDTGTVDAPTVRRYLARLRVPLYVWRLALEEPPAAADWPEAVDASTIDRLGEAFG
                     ALRDDLKSQRVVWLEDRFQPSTVDVTAKAADLVVAR"
     gene            3271..>3557
                     /locus_tag="P5164_17485"
     CDS             3271..>3557
                     /locus_tag="P5164_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HRY45727.1"
                     /translation="MDARGPLPEEGLTRREVLALIGAGSAALVLPGCSVSPPLSSART
                     AAIMDAEAWGGLAVSLDGDKAYAARVEGTLPKGLRGTLYRNGPGLFDRGGKR"
BASE COUNT          408 a         1340 c         1295 g          514 t
ORIGIN      
        1 cgttgaaggt cgccgcggtg gaagaagggc cgtcggaagt ggtcttcgtc gtggcgggag
       61 aggccctcga cgacctgcgc cgcctcatgc cctacagcag tcgctcgcag gtggcgaacg
      121 ccgccgcgga gcggatcgac ctgccgcgcg ggacgcgcta ccgcttcctg gggacggtcc
      181 cgaagacgtt cgactcgccg acggaggtcg tgagcctctt cgccgcctcc gaggacttca
      241 cgccgagaga cggctcgttc ttccgcgtcg tcggccggct cgcgatcgcg aagcgcccgg
      301 aggacccccg cgtcgccgag tccgtcgcgg tcgccggcct ctcggcggcg gccaggagcc
      361 ggcggagggc cgtcgtcctc ctcctcggca ccggcgcggc cgaggagggc ctggtcgaag
      421 ctccgcagac gcggaggttc ctctcgaggc tcggcgtccc cctccacgtc tgggacgtct
      481 cccggccgtc ggcgggggcc gcgccggacc ttccgccccc cgcggtggag cgggccgacg
      541 cgcgccccca ctggccggag gcggtccgca tcaccgacct gtcgtccctg ctgtcggcgt
      601 tccggggtgt cgagaaggag ctctcgtccc agcggatcgt ctggctgcac ggtcgcctca
      661 acccggcgaa ggtcgagctg accgcggccg ccgggaagga ggtcgcgctc gtcccatgag
      721 agccgcctcg cgttccctcg ccgtcctctt cgccggcctg ctgctcctcg ctgcgcgctc
      781 cgcggccgcc gagcacgtca cgctgccggc aggcgcgcgg ctccacctcg agcccgatcc
      841 gagctcgccc gccctcacga tgctcgacga ggaaaccctc gtcgaggtcc tcgagaggaa
      901 ggacgactgg gtccgcgtcg ggtacggcac ctggcgcggc tgggcgcggg aggccgctcc
      961 ggtctccctc ctctccgaga gaggcggacc gaacgtgccg caggagtggg tcgccggtac
     1021 gctcctcgcc cgcgcccgct ccctcctgcg ggacggcggc cgcgaggtgc cgttcgcgag
     1081 gagggccctc gtcaccgacg tgcgggacga cgcgctcctc gcgcgcctcg aggaggccgt
     1141 tctcgccgcc acggcctcgc tcggagaccg gtggaagctg cgcgtccccg tgaagtcggg
     1201 ctcctacgtc ttcctcttcg agtcggtcga ggccgagcgg aagctcggcg acgtcgcctg
     1261 cggccccatc ggcccggcgg ggtcgcgcgt cgtctcgctt gccctcgcgg gcgacggtgc
     1321 ggctccaacg ctcgagaggc tcctgcacga ggtgggccac ctctacgcgc gcgagctcct
     1381 cggggacggc gtgcccaact gggtcgagga ggggatggcc gacgccttcg tcgagggggg
     1441 cacgtcccct tcgcgctctc gctggtcgcg gcggccgctc ccggtccgcc ccgcttcgtc
     1501 ccttccgccg ccgtccgtcg cggggctgat ccgcgccggc ccggagctgt tcgagagagg
     1561 gccggcgggc gaggccctgc gcgccgagac gatctcgttc tttcgctacc tctcggaccc
     1621 ctggcttccg cgcttcaagc ccttccggag ctatctccgg agaggcagcg gcggaaagcc
     1681 tctcgaccag cccgcgctcg agaaggcgct cggggagagc ctcgccgggc tcgagcgaac
     1741 ctggcgggcg taccgggggc ggggggagta gggccgggcg tctccgcgag cggacgcccc
     1801 aggcgcgcac ggttcgtgca tcctcttgcc gagaggccga ttcaggtgat cgcgtttctg
     1861 accctctttc tcggcatcgt cgtgggtccg catcccgtcg agctctcggc cgaaccgggc
     1921 gtcgtccgcg tcgagctctc cctcgatgga gcgctggtcg cgagcctggg cccgccctgg
     1981 gcgaccacgc tcgacctcgg ccgggagatc gcgccgcggg agctcgtcgc tgtcgcctac
     2041 gacgccgccg ggaagcgcct cggcgaggcg agccagtgga tcaaccgcgc ccggccggaa
     2101 gcagaggccg gcttcgccct caagcgcgac gggtccggcc gcgtgtcggc ggcgcgcctc
     2161 ttctggcgct gccccgtgag ccccgatccg gagtcgatcg cggtcacgct cgacggggtg
     2221 ccgctcgagg cgaaggaccc cgcccgaatc cggatcccat cccgctcccc ggacaccgcg
     2281 catatcctca tcgccgacct gaccttcccc ggaggaatcg tcgcgacggc ggtcacgacc
     2341 ttcgacggac ggacgagaaa ggagtcggtg cgggagctct ccgccgttcc gatccgaatc
     2401 ctgcccgggg cccggctccc gaaacaggtt gacgggatgc gcggctggtt cgagtcgaac
     2461 gggcaggcgc tctcggtcgt cgccgtcgag gagggccagg ccgaggttgt cttcgtccag
     2521 gcggggacga tcggcccgga cttcgagcgc cttctcgtcg agagcttcca ggccccgccg
     2581 ggagcgcggc ccgtcgacct gcgaaaggac tcccggttcc tcttcctctc ctcgaatccg
     2641 cgcgcgagcg tcgccccgaa cgccgtgacg cgcctcttcc cgatctccag ggactacccc
     2701 tcccgcggcg gaggcttcct cgaggtcggg gcgaccccct tcgcgaggga gccgcggacg
     2761 cctccgcgga ttgccgaggc cgtcgccact gccggtctct ccgcgacccg gcgcgagcgg
     2821 cgccgagccg tcgtcctcct cctcggcgcc ggcgcgcggg acacgggcac cgtcgacgcc
     2881 ccgaccgtcc gccgctacct cgcgcgcctc cgggttcccc tgtacgtctg gagactcgcg
     2941 ctcgaggagc ctccggccgc cgcggactgg ccggaagccg tcgacgcctc gacgatcgac
     3001 cgcctcggcg aagccttcgg cgccctccgc gacgacctga agtcccagcg ggtcgtctgg
     3061 ctcgaggacc gcttccagcc ttccacggtc gacgtgacgg cgaaggcggc ggacctcgtc
     3121 gtggcgcggt gagcccgggt ttcgctcgcc gccttcctcc gcgccccggc cctcacgagc
     3181 cggggcgcgg gcgggctcgg cggcaggggc cgggaggcga agccgagtgt ggctgccgcg
     3241 cggcatgagc aagctgctaa cgtttccgcg atggacgctc gcgggccgct cccggaggag
     3301 ggtctcaccc gccgggaggt cctcgccctg atcggggcgg gctcggcggc gctcgtgctg
     3361 ccgggctgct cggtgagccc gccgctgtcg tcggcgcgga cggcggcgat catggacgcc
     3421 gaggcgtggg gcgggctcgc ggtgagcctc gacggagaca aggcctacgc cgcgcgtgtc
     3481 gaggggacgc tcccgaaggg gctccgcggg acgctctacc ggaacgggcc ggggctcttc
     3541 gaccggggcg ggaagcg
//
LOCUS       DAOTEZ010000443         3543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_19941_length_3543_cov_6.973624, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000443 DAOTEZ010000000
VERSION     DAOTEZ010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19941_length_3543_cov_6.973624"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="P5164_17490"
     CDS             <1..270
                     /locus_tag="P5164_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HRY45728.1"
                     /translation="PADRVVQVHLAGHTNLGTHLLDTHSDHVRDEVWALFARLVRRTG
                     PVTTLVEWDEEIPPLDVVLAEARKARAVAEAALGDRDGRREREAA"
     gene            267..1121
                     /locus_tag="P5164_17495"
     CDS             267..1121
                     /locus_tag="P5164_17495"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HRY45729.1"
                     /translation="MTAAGLPLAEFQEWMQAVIVHPGGVDAGLRSRAARRRLPPSRLG
                     AVVRAYGALSPVERLGIYASMYPLRTVEALRSDYPALATLLGERRFGALVREYVAAHP
                     STSFTLARLGDRLPSFAATWGPERGRGLRADVARLERAAAAVFDAADVALLDAPALAA
                     ALASEGESLRLVTAPAFAILAVRPGAVAVLDAVLEDASAPTSAERGLVHVALYRRDFT
                     VLRRTLDPVPGRLLAALVSGLPLAEALSRAARRRPGARIVSGWLAEWSALGAFARAGV
                     APDPSAAR"
     gene            complement(1118..1585)
                     /locus_tag="P5164_17500"
     CDS             complement(1118..1585)
                     /locus_tag="P5164_17500"
                     /inference="COORDINATES: protein motif:HMM:NF024953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRY45730.1"
                     /translation="MTRRLLAARSFVLRVSSSLSWLPLAATRVALGTAFVQSGWGKLH
                     DLPRVVGYFESLGIPAPQLQAPFVAGVELAGGALLLAGLFTRLASVPLAATMVVALLT
                     ARRADLASASDLFGAVELLYLLLLGSLAGFGAGALSLDALLVRRFAPAEEPKG"
     gene            1680..2705
                     /locus_tag="P5164_17505"
     CDS             1680..2705
                     /locus_tag="P5164_17505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45731.1"
                     /translation="MRVTIGAVGRGGTRAVGGVRAAVALLALLAACPAATQELTLFGG
                     ASTARDLDESTYAWKIEYRQGLGEHFEVTYSWLNEGHVTGHHRDGHGLQLWARERLRG
                     GRLSLAVGAGAMRFYDTVDSHPWRPYANEHGFAGLFSADAAYYFGNRWVVRAEANVAW
                     APPSSTDAWNVLAGLGYQLAPPEAPGSRPWPARQEEPTTRNEVTAFVGQTVVNGFESA
                     KALAWAVEFRKGFGRFFDASVSYVHEGDSRLVRRAGFGVQGWLGRAFLDRRLTVSLGV
                     GVYLATDEYKPLHTEGDSRTVAGLITPSVSYRFGSSALARFSWNRMASSYHRDTDVFL
                     LGVGRRF"
     gene            complement(2733..>3543)
                     /locus_tag="P5164_17510"
     CDS             complement(2733..>3543)
                     /locus_tag="P5164_17510"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRY45732.1"
                     /translation="TNEVLVGKAIRGRRSEAVLATKFGNMRGADGAFLGVNGRPEYVR
                     SCCEGSLARLGVAVIDLYYQHRVDKSVPIEETVGAMSDLVREGKVRFLGLSEASPATI
                     RRAHAVHPISALQTEYSLWTRDPEEGLLAVCRELGIGFVAYSPLGRGFLTGRFRRFED
                     LPEGDYRRLAPRFQGDNFAKNLDVLAKVEEISREKGCTPAQLALAWLHAQGADVVPIP
                     GTKSRARLEENVGALDVSLSLADLSRIEAVAPKGFTAGPRYPEASMKAIDA"
BASE COUNT          441 a         1307 c         1327 g          468 t
ORIGIN      
        1 ccggccgacc gggtcgtcca ggtccacctc gcggggcaca ccaacctcgg gacgcacctc
       61 ctcgacacgc actccgacca cgtgagggac gaggtctggg cgctcttcgc gcggctcgtc
      121 cggcgcaccg gccccgtgac gacgctcgtc gagtgggacg aggagattcc tccgctcgac
      181 gtcgtcctcg ccgaggcgcg aaaggcccgg gccgtggccg aggccgccct cggcgaccgc
      241 gacgggcgtc gcgaacggga ggcggcgtga cggcggccgg cctccccctc gcggagttcc
      301 aggagtggat gcaggcggtg atcgtccacc ccggcggggt cgacgcgggg ctccgttcgc
      361 gcgccgcgcg gcggcgcctc ccgccgtcgc ggctcggtgc cgtcgtccgc gcgtacggag
      421 cgctctctcc cgtcgagcgc ctcgggatct acgcgtcgat gtaccccctc cggacggtcg
      481 aggcgctccg ctccgactac ccggccctcg cgacgctcct cggcgaaagg aggttcggcg
      541 cgctcgttcg ggagtacgtc gcggcgcatc cctccacgag cttcacgctc gcccggctcg
      601 gcgaccggct cccgtcgttc gctgcaacgt gggggccgga gcgtgggcga ggccttcgcg
      661 cggacgtcgc ccgcctcgag cgcgccgcgg cggccgtctt cgacgccgcc gacgtcgcgc
      721 tcctcgacgc ccccgcgctg gcggccgcgc tcgcgtcgga gggggagtcg ctccgcctcg
      781 tcaccgcgcc cgccttcgcg atcctcgccg tccggcccgg cgccgtcgcg gtcctcgacg
      841 ccgtcctcga ggacgcttcg gcccccacct ccgcggagcg aggcctcgtc cacgtcgctc
      901 tctaccggag ggacttcacc gtcctccgcc gcaccctcga cccggtgcca ggtcggctcc
      961 tcgcggctct cgtgtcggga ctcccgctcg ccgaggcgct ctcccgcgcg gcgcgcaggc
     1021 gcccgggggc ccggatcgtc tcgggctggc tcgcggagtg gagcgcgctc ggcgccttcg
     1081 ctcgggccgg ggtcgccccc gacccgagcg ccgcgcgcta acccttcggc tcctccgccg
     1141 gcgcgaagcg ccggacgagg agcgcgtcga gcgagagcgc ccccgccccg aaccccgcga
     1201 gcgacccgag gaggaggagg tagaggagct cgaccgcccc gaagaggtcg gaggcgctcg
     1261 cgaggtcggc gcgcctcgcg gtcaggaggg cgacgaccat cgtcgccgcg agcgggacgg
     1321 aggcgagacg cgtgaagagc ccggcgagga ggagcgcgcc tccggcgagc tcgacgcccg
     1381 ccacgaacgg cgcctgcagc tgcggggccg ggatgccgag gctctcgaag tagccgacga
     1441 cgcgcggcag gtcgtgcagc ttcccccagc ccgactggac gaaggccgtc ccgagcgcca
     1501 cgcgcgtggc ggcgagcggg agccaggaga gggaggagga gacacggagg acgaacgagc
     1561 gggcggcgag caggcggcgg gtcatcggat ctccttcacg gctccgatgc cggaggaggc
     1621 cgcgcggttt ccggcgcggg gccggcggga cggcgccggg tggcatgatc cggactctca
     1681 tgcgcgtcac aatcggggcg gtcggccgtg gaggcacgcg cgccgtcggc ggcgttcggg
     1741 ccgccgtcgc gctcctcgcg cttctcgccg cgtgccccgc ggccacccag gagctcacgc
     1801 tcttcggcgg cgcgtcgacc gcgcgcgacc tcgacgagag cacctacgcc tggaagatcg
     1861 agtaccgcca ggggctcggc gagcacttcg aggtcacgta ctcgtggctg aacgaggggc
     1921 acgtgacggg tcaccaccgg gacggtcacg ggctgcagct ctgggcccgc gagcggcttc
     1981 gcggcggtcg gctctcgctc gcggtcgggg ccggggcgat gcggttctac gacacggtgg
     2041 actcacaccc ctggcggccc tacgcgaacg agcacgggtt cgccgggctc ttcagcgccg
     2101 acgcggccta ctacttcggg aatcgctggg tcgtacgggc cgaggcgaac gtcgcctggg
     2161 cgcctccgtc gagcaccgac gcctggaacg tcctcgcggg cctcggctac cagctcgcgc
     2221 ctccggaagc gcccggatcg cgtccctggc ctgcgagaca ggaggagccg acgacccgca
     2281 acgaggtgac ggcgttcgtg ggccagaccg tcgtcaacgg cttcgagagc gcgaaggcgc
     2341 tcgcctgggc cgtggagttc cggaaggggt tcggccgctt cttcgacgcc tccgtctcat
     2401 acgtccacga gggggactcg cggctcgtcc gtcgggcggg gttcggcgtg cagggctggc
     2461 tcggacgggc gttcctcgac cggaggctca cggtctcgct cggcgtcggc gtctacctcg
     2521 cgaccgacga gtacaagccg ctgcacacgg agggtgattc gcggacggtc gccggcctga
     2581 tcacgccgag cgtgagctac cggttcggct cgagcgccct cgcccgcttc agctggaacc
     2641 ggatggcgag ctcgtaccat cgcgacacgg acgtcttcct cctcggcgtc ggccggcggt
     2701 tctgagcggc accccggtcg gcgccccgcg cctcaggcgt cgatcgcctt catcgacgcc
     2761 tccgggtagc gcggccccgc ggtgaagccc ttcggggcga cggcctcgat ccgcgagagg
     2821 tcggcgaggg agagcgagac gtcgagcgcg ccgacgttct cctcgaggcg ggcgcggctc
     2881 ttcgtgccgg ggatcgggac gacgtcggcc ccctgcgcgt ggagccaggc gagggcgagc
     2941 tgcgccggag tgcacccctt ctcgcgggag atctcctcga ccttcgcgag gacgtcgagg
     3001 ttcttcgcga agttgtcgcc ctggaagcgc ggggcgagtc ggcggtagtc cccctccgga
     3061 aggtcctcga agcgccggaa gcgccccgtg aggaaccccc gcccgagcgg gctgtaggcg
     3121 acgaacccga tgccgagctc gcggcagacg gccaggaggc cctcctcggg gtcgcgcgtc
     3181 cagagggagt actcggtctg gagcgcggag atcgggtgga cggcgtgcgc ccggcggatc
     3241 gtggcggggg aggcctcgga gagcccgagg aagcggacct tcccctccct cacgaggtcg
     3301 gacatcgcgc cgacggtctc ctcgatcggg acggacttgt cgacgcggtg ctggtagtag
     3361 aggtcgatca ccgcgacccc gagccgcgcg agggagccct cgcagcacga ccggacgtac
     3421 tcgggccggc cgttcacgcc gaggaacgcg ccgtcggccc cgcgcatgtt cccgaacttc
     3481 gtcgcgagga cggcctcgct ccgccggccg cggatcgcct tgccgacgag gacctcgttc
     3541 gtg
//
LOCUS       DAOTEZ010000444         3519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_20145_length_3519_cov_5.502887, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000444 DAOTEZ010000000
VERSION     DAOTEZ010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20145_length_3519_cov_5.502887"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /gene="rnc"
                     /locus_tag="P5164_17515"
     CDS             <1..581
                     /gene="rnc"
                     /locus_tag="P5164_17515"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRY45733.1"
                     /translation="TLEFLGDSVLGFLVAEMLLERFPDFREGQLSKARSHLVSEGSFA
                     RIARGLGVGELLLLAAGESRSGGRVKESLLADAFEAVFAAITLDAGLPAAREVARRLF
                     EGPVSELDPGELSFHDYKTALQELVQGEGKPLPVYRLVSESGPDHRKEFVFEVVFDDG
                     LSATGSGGTKKEAQRQAAKTALAALLGRATRA"
     gene            complement(634..2427)
                     /locus_tag="P5164_17520"
     CDS             complement(634..2427)
                     /locus_tag="P5164_17520"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRY45734.1"
                     /translation="MTPRLARRLAAALATLLLCLPAAAQIGAPPEVLSLRLAADTEKI
                     VAGSSFRVALVATIAPGWHVNANTPLEDYLIPTEGALAPAEGLKFGPPAYPAYREAKL
                     PFSDKPLALYDGETVIVFEGTADAAAAPGPRTLRATLDYQPCNDAQCLAPNQSEATLE
                     IEVVPAGTPAAPANAALFPLAGARSPEEGGATEPAAGTAPAPESGPFSGRSLPAILGL
                     VFLAGLALNLTPCVYPLIPITVGFFSRQSEGKTSRTFGLAVAYVLGMSVTYSALGVFA
                     ALSGSLFGSWLQKPAVLVVIALVVLALALSMFGLYELQAPHFITDRTGSKGGVLGALT
                     MGLFVGFVAAPCIGPFVLSLLTYVAQQGSAPLGFALFFTLAMGLGLPYLVLGTVSGSL
                     KAMPRSGEWMIAVRKVFGFVLVALAAWFLRPLLPERAFAWAVALPLLAGGVWFLFFEK
                     AGAGVRWFRGLKVALGLALLAVGAWFVVPSKHAEGLAFQPYSDAALASASAAGKPVVI
                     DFFADWCLPCKELEKFTFTDPAVAKALDGWVLLKADLTKSASPEVAALRTRWNVQGVP
                     TIVFLGPDGKESLPRVVQFEKPAAFLSRFPK"
     gene            complement(2439..3320)
                     /locus_tag="P5164_17525"
     CDS             complement(2439..3320)
                     /locus_tag="P5164_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45735.1"
                     /translation="MRELRRTLLAPALLFAPLLLAQGPQTAFPSEGTDAPLDWATTMP
                     DALKRAGALDGGRLLVEIRDAQCPECERMEKLLYPSASFRGFLKDKVPVRLLRGTPEA
                     DQLLARYRLRTTPAWLVLSTDLMLCGKQEGDTNQSTWIERFFETEKAWAAFLRSIDDE
                     KANPADPARAFAVGEEAYRRYGDVMAERRFRKVSEDEQAPSELRAKSLAYLATFALEA
                     RRFDDAAKTLEKVLATSADPALLERAELRLADVEIGRGNREKAAARLNAFVEKHPQSP
                     LKAQAEELLRLLATARP"
     gene            3441..3516
                     /locus_tag="P5164_17530"
     tRNA            3441..3516
                     /locus_tag="P5164_17530"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3474..3476,aa:Phe,seq:gaa)
BASE COUNT          521 a         1122 c         1414 g          462 t
ORIGIN      
        1 agacgctcga gttcctgggc gacagcgtcc tcgggttcct cgtcgccgag atgctcctgg
       61 agcgcttccc cgacttccgc gagggccagc tctcgaaggc ccggtcgcac ctcgtgtcgg
      121 aggggagctt cgcgcggatc gcgcgcgggc tcggcgtggg cgagctgctc ctcctcgcgg
      181 cgggagagag ccggtcgggg ggccgcgtga aggagtcgct cctcgccgac gcgttcgagg
      241 cggtcttcgc ggcgatcacg ctcgacgccg gccttccggc ggcgcgcgag gtggcgcggc
      301 ggctcttcga ggggccggtc tccgagctcg atccgggcga gctgtcgttc cacgattaca
      361 agaccgcgct ccaggagctc gtgcagggcg aggggaagcc gcttcccgtc taccggctcg
      421 tctccgagtc cggccccgac caccggaagg agttcgtctt cgaggtggtc ttcgacgacg
      481 ggctctctgc gaccgggagc ggcgggacga agaaggaagc ccagcggcag gcggcgaaga
      541 ccgcgctcgc ggcgctcctc ggccgagcga cgcgcgcctg agaagcgggg cgcgcgggag
      601 agggccgtcc ggtcccctcc cgcgcgcggg agctcacttc gggaagcgcg agaggaacgc
      661 cgccggcttc tcgaactgga cgacgcgcgg gagcgactcc tttccgtccg ggccgaggaa
      721 gacgatcgtc gggacgccct gcacgttcca ccgcgtccgg agggcggcca cctcggggga
      781 ggcgctcttc gtgaggtcgg ccttcaggag gacccacccg tcgagggcct tcgcgacggc
      841 cgggtcggtg aacgtgaact tctcgagctc cttgcagggg aggcaccagt cggcgaagaa
      901 gtcgatgacg accggcttcc cggcggccga ggccgaggcg agcgccgcgt cggaataggg
      961 ctggaacgcg agtccctccg cgtgcttcga cgggacgacg aaccaggcgc cgacggcgag
     1021 gagcgcgagg ccgagcgcga ccttcaggcc gcggaaccag cggaccccgg cgcccgcctt
     1081 ctcgaagaag aggaaccaga cgccgccggc gaggagcggg agggcgacgg cccaggcgaa
     1141 cgcgcgctcg gggaggagcg ggcggaggaa ccaggctgcg agggcgacga ggacgaagcc
     1201 gaagaccttc ctcacggcga tcatccactc gccggagcgg ggcatcgcct tgaggctccc
     1261 cgagaccgtc ccgaggacga ggtaggggag gccgagcccc atcgcgagcg tgaagaagag
     1321 ggcgaagccg agcggggcgg agccctgctg ggcgacgtag gtgaggagcg agaggacgaa
     1381 cggcccgatg cagggcgccg ccacgaagcc gacgaacagg cccatcgtga gcgcgccgag
     1441 gacgcccccc ttgctccccg tccggtcggt gatgaagtgg ggagcctgga gctcgtagag
     1501 gccgaacatc gagagcgcga gcgcgaggac gacgagcgcg atgacgacga ggacggccgg
     1561 cttctggagc cacgagccga agagcgaccc ggagagcgcg gcgaagacgc cgagcgcgga
     1621 gtacgtgacg ctcattccga ggacgtaggc caccgcgagg ccgaacgtgc ggctcgtctt
     1681 cccctcgctc tggcgcgaga agaagccgac cgtgatcggg atgagggggt agacgcaggg
     1741 ggtcaggttg agcgcgaggc ctgcgaggaa gacgaggccg aggatcgcgg ggagagagcg
     1801 gccggagaac gggccggact cgggcgccgg ggcggtcccc gcggccggtt ccgtcgcgcc
     1861 tccttcctcg ggcgaccggg ctccggcgag cgggaagagc gcggcgttcg ccggggcggc
     1921 gggcgtgccg gccgggacga cctcgatctc gagcgtcgcc tcggactggt tcggcgcgag
     1981 gcactgcgcg tcgttgcagg gctggtagtc gagagtcgcg cggagcgtcc gcgggcccgg
     2041 cgcggcggcc gcgtcggccg ttccctcgaa gacgatgacg gtctcgccgt cgtagagggc
     2101 gagcggcttg tcggagaagg ggagcttcgc ctcgcggtag gcggggtagg cgggcgggcc
     2161 gaacttcagc ccttcggccg gcgcgagggc gccctccgtc gggatgaggt agtcctcgag
     2221 gggggtgttc gcgttgacgt gccatcccgg cgcgatcgtg gcgacgaggg ccacgcggaa
     2281 cgagctcccg gcgacgatct tctcggtgtc ggcggcgagt cgcagagaaa ggacctcggg
     2341 gggggcgccg atctgggccg cggcggggag gcagaggagg agggttgcga gcgccgctgc
     2401 gaggcggcgg gcgaggcgtg gcgtcattcg ggttcccttc acggtcgggc ggtggccagg
     2461 aggcggagga gctcctcggc ctgggccttc aacggggact gcgggtgttt ctcgacgaag
     2521 gcgttcagtc gcgcggccgc cttctcgcgg tttccccgtc cgatctccac gtcggcgagg
     2581 cgaagctcgg cccgctcgag gagcgcaggg tcggccgagg tggcgaggac cttctcgagg
     2641 gtcttcgcgg cgtcgtcgaa gcggcgggcc tcgagcgcga acgtggcgag gtaggcgagc
     2701 gacttcgcgc ggagctcgga aggggcctgc tcgtcctcgg agaccttgcg gaagcgccgt
     2761 tcggccatca cgtcgccgta gcgccggtag gcctcctcgc cgacggcgaa ggcgcgagcc
     2821 gggtcggccg ggttcgcctt ctcgtcgtcg atcgaacgga ggaacgccgc ccacgccttc
     2881 tcggtctcga agaagcgctc gatccaggtc gactggttcg tgtcgccttc ctgcttcccg
     2941 cagagcatca ggtcggtcga gaggacgagc caggccggag tcgtccgcag gcggtagcgc
     3001 gccaggagct gatcggcctc gggcgtcccg cggaggaggc ggaccgggac cttgtccttc
     3061 aggaagcccc ggaaggacgc ggacgggtag aggagcttct ccatccgctc gcactccgga
     3121 cactgcgcgt cgcggatctc cacgagcaga cgcccgccgt cgagggcgcc ggcgcgcttg
     3181 agggcgtcgg gcatggtcgt cgcccagtcg aggggcgcgt ccgtgccttc ggacgggaag
     3241 gccgtctgcg gtccctgggc gaggagcagc ggcgcgaaga ggagggcggg ggcgagaagg
     3301 gtccggcgca gttctctcat ttcatctcgg tcggggagcg gcggcgcgaa gggccgcgtt
     3361 gactattcta gtgggcgccg gtataacgcc cggcgtcgcg ctgaggggcg gaccgcaagg
     3421 tgcgtgccgc ggcgccgggt gcccaggtag ctcagctggt agagcacgcg cctgaaaagt
     3481 gcggtgtcga cggttcaact ccgcccctgg gcaccatct
//
LOCUS       DAOTEZ010000445         3514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_20193_length_3514_cov_4.490604, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000445 DAOTEZ010000000
VERSION     DAOTEZ010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20193_length_3514_cov_4.490604"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="P5164_17535"
     CDS             complement(<1..586)
                     /locus_tag="P5164_17535"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRY45736.1"
                     /translation="MPLAPGTRLGRYEVIAPLGAGGMGEVFRARDSKLGRDVALKVLP
                     DRFAGDRRMLARFESEAKAVAALSHPNILALFDVGEESGVPFAVAELLEGETLRALLM
                     RGPVPVKRALEVAREIAEGLAAAHAKGIVHRDVKPENVFVTKDGHAKVLDFGLARHET
                     AFRSEDESRSPTLSALTDAGAVVGTVAYMSPEQAR"
     gene            805..1083
                     /locus_tag="P5164_17540"
     CDS             805..1083
                     /locus_tag="P5164_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRY45737.1"
                     /translation="MKFVSVRDLRGRSGQVWKRLDDGGDVVVTSNGKPIAVLSPVREG
                     DLERSLATIRRARALDAVADLQAGAASRKVSPREVEAEIAAVRKGRSR"
     gene            1080..1520
                     /locus_tag="P5164_17545"
     CDS             1080..1520
                     /locus_tag="P5164_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HRY45738.1"
                     /translation="MRIVLDTNVLVSGLLSPFGPPADVVRLVAAGALRLGVDARILEE
                     YRLVLARPRFGFDAARVAELLSQVDADGVRGATQPLRLRLPDPDDEPFLEVALAVAAD
                     ALVTGNVRHFPSSRREGARVLSPRAFVEEYRGRQGRSRSPTTRA"
     gene            complement(1578..>3514)
                     /locus_tag="P5164_17550"
     CDS             complement(1578..>3514)
                     /locus_tag="P5164_17550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45739.1"
                     /translation="ERWDSTRDLARDLAIWGHRGAERSSATGAAAGADAGIPRTSRRT
                     RILLASFGALAVVTALVGGFQAGRKFEGSKRPDATSPGRLTQLTWEPGMELEPCISAD
                     GGSFVYDAGPPGNRDIFFRRVGGENPVNLTRDHTGNDIRPALSPDGSTIAFRSERDGG
                     GIFLMGASGESPRRLTDFGYDPAFSPDGKEIVFATGTATGFSGDRSVLWIADVATGKT
                     RKLFDGGGAFPTWSPSGIRIAFEQADRGTSLGGRRTLSTIPASGGAPTPVVELPSTMA
                     SCPDWSLTGITFDTSVAGQTNVWRIEVDEATGSPLGEEQPVLVSPAESLRSSSTRDGR
                     RMLFMTWEGANSIDRFDFDPVKGQLVGERETVASGPRELRPNGLSPDGQWLATLFLER
                     GGRKDIVLLRVGTGETRRLTDDPPQKDFLRWAPDGSKLYFGVAPEGLNEVWSIRPDGS
                     GRQCEIRAPKEGGVSPSVVSPDGRTLYVDVGKEVRPHRVDLIVPPERRSLVPMPRLPD
                     GRHFDTQQLSPDGRWLVGRSRAPAGDLVRDPLYLFDVERETYAALPSQPGASVLGWLP
                     DSRRLLLAVGGELRILDRVTRNVSPAGLPGAGRFTAAPRLTRDGRSIYGFRQRNEGDI
                     WMIDFADPPDRSARTARSGN"
BASE COUNT          490 a         1214 c         1303 g          507 t
ORIGIN      
        1 cgcgggcctg ctcgggggac atgtaggcga cggtgccgac gacggcgccg gcgtccgtga
       61 gggcggagag cgttggggag cggctctcgt cctcgctccg gaacgctgtc tcgtggcggg
      121 cgaggccgaa gtcgaggacc ttggcgtggc cgtcctttgt cacgaagacg ttctcgggct
      181 tgacgtcgcg gtggacgatt cccttcgcgt gggcggcggc gagcccctcg gcaatctcgc
      241 gggcgacctc gagggcgcgc ttcacgggga ccgggccgcg catcaggagg gcgcggagcg
      301 tctccccctc gaggagctcg gcgacggcga acgggacgcc gctctcttcg ccgacgtcga
      361 agagggcgag gatgttgggg tgggagaggg cggcgacggc cttcgcctcg ctctcgaatc
      421 gggcgagcat gcggcggtcg ccggcgaaac gatcggggag gaccttgagg gcgacgtcgc
      481 ggccgagctt cgagtcgcgg gcgcggaaga cctcgcccat gccgcccgcg ccgagcggcg
      541 cgatcacctc gtagcggccg aggcgggtgc cgggagcgag aggcatgcgc cggcctccgg
      601 aaatgctcga gctcggatcg ttccggcgag aagtctacga cggcggaagg gcggccggtg
      661 tcggtcgcgt atcaccctcc gccgctgtct cgtgacgccg gacgcgcggc cggctaccgc
      721 tggcagggga gcccgttcac gataacgacc atctctgagg cacgatcgtg tcacgagcgc
      781 gtatcatcgg gatggaggct cgtcgtgaag ttcgtctccg tccgcgatct caggggccgc
      841 tccgggcagg tctggaagcg gctcgacgac ggcggcgacg tcgtcgtcac ctcgaacggg
      901 aagcccatcg ccgtcctctc cccggtgcgt gagggcgacc tcgagcggtc gctcgccacg
      961 atccgcaggg cccgggcgct cgacgcggtc gcggacctcc aggccggcgc cgcgagccgg
     1021 aaggtctcgc cccgcgaggt cgaggccgag atcgcggccg tccggaaggg ccgcagccgg
     1081 tgaggatcgt cctcgacacg aacgtcctcg tgtcggggct cctctcgccg ttcgggccgc
     1141 cggccgacgt cgtccggctc gtggcggcgg gcgcgctgag gctcggcgtc gacgcgagga
     1201 tcctcgagga gtacaggctc gtcctcgcgc ggccgcgctt cgggttcgac gcggcgcggg
     1261 tcgccgagct gctttcccag gtcgatgccg acggagtccg gggcgcgacg cagcctcttc
     1321 gcttgcgcct gccggaccct gacgacgagc cgttcctcga ggtggcgctc gccgtcgccg
     1381 cggacgccct cgtgaccggc aacgtccggc acttcccgtc gtcccgccgc gagggggccc
     1441 gggtcctctc gccccgggcg ttcgtggagg agtaccgggg gaggcaggga aggagtcgtt
     1501 cgccgacgac acgggcgtga cgacccggcg acacgcggct ccggggaagc atcgactgga
     1561 actcggagga ggccgcctca attccccgac cgggccgtcc gggcggagcg gtccggcggg
     1621 tccgcgaagt cgatcatcca gatgtcgccc tcgttccgct ggcgaaagcc gtagatcgac
     1681 cgcccgtccc gggtaaggcg cggcgcggcg gtgaagcggc cggccccggg caggccggcg
     1741 ggcgagacat tcctggtgac acgatccagg atccgaagct ccccgccgac cgccagaagg
     1801 aggcgccgag agtcggggag ccagccgagc acgctcgcgc ccggctgtga cgggagtgcc
     1861 gcgtacgtct ccctctcgac gtcgaagaga tacaaggggt cgcgaacgag atcgccagcc
     1921 ggggcccgcg agcgcccgac gagccacctg ccgtccggtg acagctgctg cgtgtcgaag
     1981 tgccgcccgt cgggaaggcg cggcatcggg acgagcgacc ggcgctccgg gggaacgatg
     2041 agatcgacgc ggtgcgggcg gacctccttc ccgacgtcca cgtagagcgt ccgaccatcc
     2101 ggggagacga cactcggaga gactccaccc tccttcggcg cccggatctc gcactgtcgt
     2161 ccgctgccgt cggggcggat gctccagacc tcgttcagac cctccggcgc gacaccgaaa
     2221 tagagcttcg aaccgtcggg cgcccagcgg aggaagtcct tctggggcgg gtcgtcggtg
     2281 aggcggcggg tctctcccgt cccgaccctg aggaggacga tgtcttttcg ccctcctcgc
     2341 tcaaggaaga gcgtcgcgag ccattggccg tccggcgaga ggccattggg ccgaagttcg
     2401 cgagggccgc tcgcgacggt ctcccgctcg ccgacgagct ggcccttcac tgggtcgaag
     2461 tcgaaccggt cgatcgagtt cgccccctcc caggtcatga agagcattcg ccgcccgtcg
     2521 cgcgtcgagg acgagcggag cgattcggcc ggcgagacga ggaccggttg ctcctcgccg
     2581 agaggcgatc cggtcgcctc gtcgacctcg atcctccaga cgttcgtctg accggcgacc
     2641 gacgtgtcga acgtgatccc cgtgaggctc cagtccgggc aggaggccat cgtcgacggc
     2701 agctcgacga ccggcgtcgg ggcgcctccc gaggcgggaa tcgtcgagag cgtcctcctt
     2761 ccgccgaggg aggtcccccg gtcggcctgc tcgaaggcga ttcggatgcc cgaaggagac
     2821 cacgtcggaa atgctccgcc gccgtcgaag agctttctcg tcttccccgt ggcgacgtca
     2881 gcgatccaaa ggacgctccg gtcccccgag aagcccgtcg ccgtgccggt cgcgaagacg
     2941 atctccttcc cgtcgggcga gaaggccggg tcgtagccga agtccgtcag gcggcggggg
     3001 ctctctccgc tggcgcccat caggaagatc ccgccgccgt cgcgctcgga gcggaaggcg
     3061 atcgtggagc cgtcagggga gagcgccggt cggatgtcgt tccccgtgtg gtcgcgggtc
     3121 aggttcacgg ggttctcccc gccgacgcgc cgaaagaaga tgtcccggtt cccgggcggg
     3181 ccggcgtcgt agacgaagct cccgccgtcg gccgatatgc acggttcgag ctccattccg
     3241 ggttcccacg tcagctgcgt cagccttccc ggcgaagttg cgtccggccg cttcgacccc
     3301 tcgaatttcc gtccggcctg aaatccgccg acgagggcgg tgacgacggc gagggcgccg
     3361 aacgaggcga ggaggattcg cgtccgacga ctcgtccgcg ggatccctgc gtccgccccg
     3421 gcggcggctc cggtcgccga ggatcgctcc gcgccccggt gcccccagat ggcgaggtcg
     3481 cgcgccaggt cgcgggtcga gtcccagcgc tcgg
//
LOCUS       DAOTEZ010000446         3512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_20215_length_3512_cov_5.792884, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000446 DAOTEZ010000000
VERSION     DAOTEZ010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20215_length_3512_cov_5.792884"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="P5164_17555"
     CDS             complement(<1..928)
                     /locus_tag="P5164_17555"
                     /inference="COORDINATES: protein motif:HMM:NF018067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HRY45740.1"
                     /translation="MTLADVLLPRKGAILVKWGFEKYRSGFRRITRRSARHFEAADWH
                     GVQQDMVERLALYTSVVRRVVASLGEVFGEDRRTPGLWSAMKREYSWLMSGHGDLELA
                     ETFFNSVTRKVFATVGVDPKREFVHLGSDMGLPPAGAVPVRTYPGALSTGTVVGEVLD
                     DYACAVPWEDPARDAARVAEEVDRAVAAAWGAPGFDFVELLEPVFYRGKGAYVVGRVV
                     RAERSLPLVLALANPGGRVVVDAVLLDEDDVSIVFSFTRSYFHVDVEAPAGIVRFLKS
                     ILPRKPLAELYVSLGYNKHGKTELYRDLLRHLE"
     gene            complement(954..1292)
                     /locus_tag="P5164_17560"
     CDS             complement(954..1292)
                     /locus_tag="P5164_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011531289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HRY45741.1"
                     /translation="MPWLFLVLAGLLEVGWAVGLKFTDGFRRPLPSLLVAASMAGSVV
                     LLAWAVRSIPIGTAYAVWVGIGALGAVVLGVLLFDEPLPPLRALFVALLVVAIAGLKW
                     TSGPAGDAGS"
     regulatory      complement(1305..1370)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01763"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="guanidine-III (ykkC-III) riboswitch; guanidine
                     riboswitches control genes whose products are involved in
                     modification or pumping out guanidine as a toxic compound
                     from bacteria; this is a third class of guanidine
                     riboswitches based on ykkC-III RNA motif; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF01763"
     gene            complement(1441..3213)
                     /locus_tag="P5164_17565"
     CDS             complement(1441..3213)
                     /locus_tag="P5164_17565"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HRY45742.1"
                     /translation="MTPNTGLPTPAQTVVPGLPAGSRPHGVAYFGSDNALLSDFNQGR
                     VFNVQISTASLVSTITTSPSYGGMGSIAVAPGLAHALACGGSTTLSVLHAPFIAGATI
                     TTVALPGQVASYQTQAIGFAPSGRAFVYTTAGISVLDPPYSSIAFTIPVSGNANSGAL
                     AVSPDGNTLLVTKLTTVVEMFTAPFTAASTGSTLTVAGAQGLDGIQITPDGTKALVAG
                     VFASLQAYAISAPFTSGSLVEALPIPAACTGGFEDVGISADGQLAVFTGNSSGSRLPA
                     AFVRAPFTAAGATSFAVQLGSAGPPDTRGRGTGAVRFLPPGLAPGLTVAKSAPATVAP
                     GAQLTYTVTYGNTGGAAASGVVIRETLPAGTSFVTATGGGTESGGVVTWNVGSLGAGV
                     TGQTVSFTVTVTAQSGSIVNGTYTIEGAGIAPIFGPPVTTAVQASPAVLAIQKTASLA
                     TPVLGAPFSFRIDVTNSGGSPAAGVQVTDEVPAGLAIGTVTIVPSGSCNVAGRLVSCS
                     IPSLAAGSVATITIPVTASAAGLLTNTATAAFTAGASVQDDASVTVQDREAVATVPVA
                     SAWGLTILGIALAAAGWLAIRRGV"
BASE COUNT          563 a         1229 c         1268 g          452 t
ORIGIN      
        1 gctcgaggtg ccggaggagg tcccggtaga gctccgtctt gccgtgcttg ttgtagccca
       61 gcgagacgta gagctcggcc agcggcttgc gcgggaggat cgacttgagg aaccggacga
      121 tccccgccgg cgcctcgacg tcgacgtgga agtaggagcg ggtgaagctg aagacgatcg
      181 agacgtcgtc ctcgtcgagg aggacggcgt cgaccacgac cctcccgccc gggttcgcga
      241 gcgcgaggac caggggcagc gagcgctccg ccctgacgac gcgcccgacg acgtacgctc
      301 ccttgccgcg gtagaagacc ggctcgagga gctcgacgaa gtcgaagccg ggggcgcccc
      361 acgccgcggc gacggcccgg tcgacctcct ccgccacccg ggcggcgtcg cgcgccgggt
      421 cctcccacgg aaccgcgcag gcgtagtcgt cgagcacctc gccgacgacc gtcccggtcg
      481 agagcgcgcc gggataggtc ctcaccggaa ccgcccccgc gggggggagg cccatgtcgc
      541 tcccgaggtg gacgaactcc cgcttcgggt cgaccccgac ggtggcgaag accttccgcg
      601 tgaccgagtt gaagaacgtc tccgcgagct cgaggtcgcc gtgtccgctc atcagccacg
      661 agtactcgcg cttcatcgcc gaccagaggc cgggcgtccg gcggtcttcc ccgaacactt
      721 ccccgagcga cgcgacgacg cgccggacga cgctcgtgta gagcgccagg cgctcgacca
      781 tgtcctgctg gaccccgtgc cagtcggccg cctcgaagtg gcgcgccgag cgccgggtga
      841 tcctccggaa gcccgagcgg tacttctcga agccccactt cacgaggatc gctcccttgc
      901 gcgggaggag gacgtccgcg agggtcatcg tgccgcgagg atacggcagg ggctcagctc
      961 ccggcgtccc ctgcgggacc cgaggtccac ttcagcccgg cgatcgcgac gacgaggagc
     1021 gccacgaaga gcgcccggag aggcggaagc ggctcgtcga agagaaggac tccgagcacg
     1081 accgcgccga gcgcgccgat cccgacccag acggcgtagg ccgtcccgat cgggatcgag
     1141 cggaccgccc acgccaggag gacgacgctc ccggccatcg aggcggcgac gaggagcgac
     1201 ggaagggggc ggcggaagcc gtcagtgaac ttcaggccga cggcccaccc cacctcgagg
     1261 agaccggcca ggacgaggaa gagccagggc atgggaggct cctttccgcg tcgtcttgtc
     1321 ctgaccgggt acggcgcgtc tcgtccgggc gacacggctc gcggccgcgc gcggcgcgaa
     1381 ccgtagcatc gcggcccggg gcgttcaact ccgccccggg ccgcggcgcc cggggccgct
     1441 tcagactcct cgccggatcg ccagccagcc ggcggcagcg agcgcgattc cgagaatcgt
     1501 cagtccccag gccgacgcga cgggcaccgt cgccaccgcc tcgcggtcct ggaccgtcac
     1561 cgaggcgtcg tcctgcaccg aggcgccggc cgtgaaggcg gccgtggccg tgttcgtcag
     1621 caggcccgcg gcggaggcgg tcaccgggat cgtgatcgtt gcgacgcttc ccgcggcgag
     1681 ggaggggatc gagcagctga cgagccgtcc ggcgacgttg cagctgcccg atggcacgat
     1741 cgtcaccgtg ccgatcgcga gcccggcggg gacctcgtcc gtgacctgga ccccggcggc
     1801 cggcgaaccg cccgagttcg tgacgtcgat ccggaagctg aacggggcgc cgagaaccgg
     1861 cgtcgcgaga gaggccgtct tctggatcgc gagcacggcc ggagacgcct gcaccgccgt
     1921 cgtcaccgga gggccgaaga tcggagcgat gcccgcccct tcgatcgtgt aggtgccgtt
     1981 cacgatgctc ccgctctgcg cggtgaccgt gaccgtgaac gacaccgtct ggcccgtgac
     2041 accggccccg agggagccga cgttccacgt gacgactccg ccgctctccg tccctccgcc
     2101 ggtggccgtg acgaacgacg taccggcggg aagcgtctcc cggatcacga cgccgctcgc
     2161 ggccgcgcct cccgtgttcc cgtaggtgac cgtgtaggtg agctgcgcgc ccggtgcgac
     2221 cgtcgcgggc gcgctcttcg cgaccgtcag gccgggcgcg agacccggcg gcaggaatcg
     2281 tacggcgccc gtcccgcggc cccgggtgtc cggcggacct gccgagccga gctggacggc
     2341 gaacgaagtc gccccggccg ccgtgaacgg agcccgtacg aacgcggccg ggagccggct
     2401 ccccgagctg ttgcccgtga agacggcgag ctgaccgtcc gcgctgatgc cgacgtcctc
     2461 gaatccgccg gtgcaggccg ccggaatcgg aagcgcctcc acgagcgacc cggaggtgaa
     2521 gggggccgag atcgcgtacg cctgcaagga cgcgaacacg cccgccacga gcgccttcgt
     2581 cccgtccgga gtgatctgga tcccgtcgag cccctgtgcc ccggcgaccg tcagggtgct
     2641 tccggtggag gccgccgtga agggggccgt gaacatctcg acgacggtcg tcagcttcgt
     2701 gaccaggagc gtgtttccgt cgggcgagac ggcgagagcg ccgctgttcg cgttccccga
     2761 gaccgggatc gtgaacgcga tggacgagta gggggggtcg aggaccgaga tcccggccgt
     2821 cgtgtagacg aacgctcgac ccgaaggggc gaagccgatc gcctgcgtct gataggaggc
     2881 cacctggccg ggcagggcga cggtcgttat cgtcgctccg gcaatgaacg gggcgtgcag
     2941 gacgctcagg gtggtcgacc cgccgcaggc cagcgcgtgc gcgagccccg gcgccacggc
     3001 gatcgaaccc atcccgccat aggacggcga ggtcgtgatc gtcgagacga gggaggccgt
     3061 cgagatctgg acgttgaaga cgcgcccctg gttgaagtcc gagaggagcg cgttgtccga
     3121 gccgaagtac gcgacgccgt gcggtctcga gcccgcggga aggcccggca ccacggtctg
     3181 agccggcgtc ggaaggcccg tgttcggcgt catcagaacg atcgcgacgt tcccgtcgct
     3241 ttccgtcccg aagattgcct ggccgtgaac gggcatcgct ccgagcacac ccgtcacaag
     3301 gaccggcaag agccggccaa agagacccac tcgcatttgt tcccccttgc tgaaacggtt
     3361 aaggatagca atctatcggc tcctccacaa gaggattctt gcgaggcgag gggaaggagt
     3421 ccccggagtg tccagggatg agccacaccg gcattggatc ggtgatggaa aggaccaccc
     3481 ccgacgcgga gcccgcgcta gcccggcggg ta
//
LOCUS       DAOTEZ010000447         3510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_20227_length_3510_cov_7.659045, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000447 DAOTEZ010000000
VERSION     DAOTEZ010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20227_length_3510_cov_7.659045"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /locus_tag="P5164_17570"
     CDS             complement(<1..1466)
                     /locus_tag="P5164_17570"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRY45743.1"
                     /translation="MRRLPAAALAASLPLLASCTASLPRDLRLVPASEPLARRVAGLA
                     EESGARMGVVALHVESGRELRWRDTETFEGASVVKLALLVEALARSREGTLELDERWP
                     MPPAAVAAGSGILDEFGPWLAPTQRDLLRIMMVLSDNTAANRFIDLFGAEAVNCRMAS
                     IGLAGIELVGRIPDAGSPEDDAPWKPLGRMTPRDTAELWRRAATGQLLDRDASRIAMR
                     LAARPRTANRIPVQLSSRPGYAWTGKSGTMRGVRADSGVLVTPKGTFVFAMFADHLPD
                     RAEAGPAANAAMGAIAREIVDDWSRTLPDRAPIDLSAERPVQPALARIERTPLEARAG
                     APDLERVFRETDRTFWELWERAGGDLSDACLRPMPNSWWEGFQPERIEPLSALVLHHT
                     AGDEDEYALSIFEDPARFVSSHFLVGKDGRLWQLVSLEHRAWHAGSSWLHGRSVLNRS
                     SVGVEITGDGNRYPFTRAQIETVVRLVGVLTAQLGLEAP"
     gene            complement(1463..3256)
                     /locus_tag="P5164_17575"
     CDS             complement(1463..3256)
                     /locus_tag="P5164_17575"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HRY45744.1"
                     /translation="MEIANGPVPTGELPARVFDLAGIALALADASGRIEWANEAFRAL
                     HRVPADGGPGLSLRDLVVGRPGTPPPLPEPLTLGFQGVVRHRRLDGTLFFGELTVRPA
                     GSGVSVVVRDASEDRRAAAVHDCLLALLRTAHENPSPGELYPVAHALVGRLLPATGFA
                     VVGLDPATARVELLYSAPEKLGQDVREAVWSVAGEVIASRAPLSLGSAGRDGEATARR
                     AGPAGVPGTSWLGAPLGDDLGALVVWASGGAGHDEADGALLGTLAPLVGEALRRGREE
                     ARRQGELRAKSALMEALSDAGQALVTLRDGFVVSANDAALRLAGTTPEELFGRRLLLE
                     LAPPAERTHLARLLASPPQEPFETALALPGGERREVQMAIRAVDLPDGLLHLAVFHDV
                     TALVRSARTDWLTSLPNRRSTEDVLAREWERLRRRSGAFALSRGELTDARPVTPLSVV
                     MIDIDDFSVFNDRHGHHTGDEMLRRTALVLRSALRSCDIVGRWGGEEFLAILPETDDV
                     GAVAVAERLRHAVEADAFYDLPRFPSGEGTTSEAVRLKVTVSLGVATETRPAAATAQG
                     LVRRADAALYAAKAAGKNRVVPAPPEADSAG"
     gene            3309..>3510
                     /locus_tag="P5164_17580"
     CDS             3309..>3510
                     /locus_tag="P5164_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HRY45745.1"
                     /translation="MSESQPPAGRLVALDLFRGATVAAMLFVNNPGSWAHVLPPFGHA
                     EWHGCTPTDLVFPFFLFIVGTAS"
BASE COUNT          464 a         1239 c         1381 g          426 t
ORIGIN      
        1 ggcgcctcga ggccgagctg ggccgtcaga acgccgacga gcctcacgac ggtctcgatc
       61 tgggcccgtg tgaacggata gcgattgccg tcgccggtga tctccacgcc gaccgagctt
      121 cggttcagga cggagcggcc gtggagccag gaggagccgg cgtgccaggc ccggtgctcg
      181 agggagacga gctgccagag ccggccgtcc ttcccgacga ggaagtgaga ggagacgaag
      241 cgggccggat cctcgaagat cgagagggcg tactcgtcct cgtcccccgc cgtgtggtgg
      301 aggacgagcg cggaaagcgg ctcgatccgc tccggctgga acccctccca ccaggaattc
      361 ggcatcggcc ggaggcaggc gtcggagaga tccccgcccg cacgttccca gagctcccag
      421 aaggtccggt cggtctcgcg gaagacccgc tcgaggtcgg gggcccccgc tcgcgcctcg
      481 agaggcgtcc gctcgattcg tgcgagcgcc ggctggacgg gacgctcggc cgagaggtcg
      541 atcggtgcgc ggtcggggag ggtccgggac cagtcgtcga cgatctctct ggcgatcgcc
      601 cccatcgccg cgttcgcggc ggggccggcc tcggcgcggt cgggaaggtg gtcggcgaac
      661 atcgcgaaga cgaacgtccc cttcggcgtg acgaggacac cggagtcggc gcggacgccg
      721 cgcatcgtcc ccgacttccc ggtccaggcg tacccgggcc gagaggagag ctggaccggg
      781 atccggttcg cggtccgggg gcgggcggcg agccgcatcg cgatccggct cgcgtcgcga
      841 tcgaggagct gcccggtcgc cgcgcggcgc cagagctcgg cggtgtcgcg cggggtcatc
      901 cgcccgagcg gcttccaggg cgcgtcgtcc tcgggcgacc cggcgtcggg gatccggccg
      961 acgagctcga tcccggcgag gccgatcgac gccatccggc agttcacggc ctcggctccg
     1021 aagaggtcga tgaaccggtt ggccgcggtg ttgtcggaga ggaccatcat gatccgcagc
     1081 aggtcgcgct gcgtcggcgc gagccacggc ccgaactcgt cgaggatgcc ggagccggcc
     1141 gcgacggcgg ccgggggcat cggccagcgc tcgtcgagct cgagcgtccc ctcgcggctc
     1201 cgcgcgagcg cctcgacgag gagcgcgagc ttcacgacgc tcgccccttc gaaggtctcc
     1261 gtgtcgcgcc accggagctc gcgcccgctc tcgacgtgga gggcgacgac ccccattcgc
     1321 gcgccgctct cctccgcgag ccccgcgacg cgtcgcgcga gcggctccga ggccgggacc
     1381 agccggaggt cgcgggggag ggacgcggtg caggaggcga ggagcgggag cgacgcggcc
     1441 agcgccgccg cggggagacg tctcatcccg cggagtctgc ctccggagga gccgggacga
     1501 cacggttctt tccggccgcc ttcgccgcgt agagcgcggc gtcggcccgc cggacgaggc
     1561 cctgggccgt cgcggcggcg ggccgggtct cggtggcgac gccgaggctg acggtcacct
     1621 tcaggcggac ggcctcggag gtcgtgccct cccccgacgg gaagcgcggg aggtcgtaga
     1681 aggcgtcggc ctcgacggcg tgccggagcc tctcggcgac ggcgacggcg ccgacgtcgt
     1741 cggtctcggg gaggatcgcg aggaactctt ctccgcccca tcgcccgacg atgtcgcagg
     1801 agcggagcgc ggagcgcagg acgagcgccg tccgccggag catctcgtcg ccggtgtggt
     1861 gcccgtgccg gtcgttgaag accgagaagt cgtcgatgtc gatcatgacg acggagagcg
     1921 gcgtcacggg gcgggcgtcg gtgagctccc cgcgggagag ggcgaatgct cccgaccggc
     1981 ggcgcaggcg ctcccactcc cgggcgagca cgtcctccgt ggaccggcgg ttcggcaggg
     2041 aggtgagcca gtcggtccgc gccgaccgga cgagcgcggt gacgtcgtgg aagacggcga
     2101 ggtggaggag gccgtcgggc aggtcgacgg cccggatcgc catctgcacc tcgcgccgtt
     2161 cgccgccggg aagggcgagg gccgtctcga acggctcctg cgggggagac gccaggaggc
     2221 gcgccaggtg cgtccgctcg gcgggaggtg cgagctccag gaggagccgg cggccgaaga
     2281 gctcttccgg cgtcgtcccg gcgaggcgga gggcggcgtc gttcgcggag acgacgaagc
     2341 cgtcgcggag cgtcacgagc gcctggccgg cgtccgagag cgcctccatc agggcggact
     2401 tcgcgcggag ctcgccctgc cgccgcgcct cctcccggcc gcggcggagc gcctcgccga
     2461 cgagcggcgc gagcgtcccg agcagcgccc cgtcggcctc gtcgtggccg gctccgccgg
     2521 aggcccagac gacgagcgcg ccgaggtcgt ccccgagcgg ggcgccgagc caggaggtcc
     2581 ccgggacgcc ggcggggccg gcccttcgcg cggtcgcctc cccgtcgcgg ccggcggacc
     2641 cgagactgag gggcgcgcgg gaggcgatca cctcgcccgc gacgctccag acggcctcgc
     2701 ggacgtcctg tccgagcttc tcgggcgccg agtagaggag ctcgacccgc gcggtcgccg
     2761 ggtcgaggcc gacgacggcg aaccccgttg cgggcaggag ccggccgacg agggcgtgcg
     2821 ccaccgggta gagctctccg ggggaggggt tctcgtgcgc ggtccgaagg agcgcgagga
     2881 ggcagtcgtg gacggccgcc gcccggcgat cttcggaggc gtcccggacg acgaccgaga
     2941 cgccgctccc cgcgggccgg acggtcagct ctccgaagaa gagcgtgccg tccaggcgcc
     3001 ggtgccggac gaccccctgg aagccgagtg tgagcggctc cggaaggggc gggggcgtcc
     3061 cggggcggcc gacgacgagg tcgcggagag agaggcccgg cccgccgtcg gcggggacgc
     3121 ggtgaagggc gcggaaagcc tcgttcgccc attcgatccg gccggacgcg tcggccagag
     3181 cgagggcgat cccggcgagg tcaaagactc gggcgggcag ctctcccgtc ggcaccggac
     3241 cgttcgcgat ctccatgccc gtcgaggata gatcgtccgc ggtccgcacg gttccctaga
     3301 atcgggccct gtccgaatcg cagcctcccg cgggccggct cgtcgccctc gacctcttcc
     3361 ggggagcgac cgtcgccgcg atgctcttcg tcaacaaccc ggggagctgg gcgcacgtcc
     3421 tcccgccctt cggccacgcg gagtggcacg gctgcacgcc gaccgacctc gtcttcccgt
     3481 tcttcctctt catcgtcggg acggcctcgg
//
LOCUS       DAOTEZ010000448         3509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_20236_length_3509_cov_6.390562, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000448 DAOTEZ010000000
VERSION     DAOTEZ010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20236_length_3509_cov_6.390562"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1347
                     /locus_tag="P5164_17585"
     CDS             <1..1347
                     /locus_tag="P5164_17585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRY45746.1"
                     /translation="WLLVEALTRDLRVEVTAVATSVGKDVVRVLQRRDGKLLELGGDA
                     GVRRIVVRDEAHVVTLPPPSAAEAPAKDRTAGPEAGPKEAEGPRFVVTTVRTEGSGTT
                     SSTKTWTTKGGTGFLFLEGPVEKVKIPIPGTGVKEAVGRFRTRLVLGSLGIVAFGLLA
                     AAFVAHRVTRPLAELSRAARTVGEGAFGTRVETREGGEVGAAIAAFNQMSSHLLALER
                     EALALKEREHLGEMGEVARGMAHALRNPLHAVGLSVDELAARLPDDPDAAAMAAAARR
                     QIAGVDGTIRSFLALAAGGGAEEEVDVRALAEEVALAALQEARGRVRVSVAPGGAARL
                     RAVAPELKAALHALVVNAVEASPDGGEVVVSVAGEEGRTVVRVEDEGPGLPEEVRARL
                     FTPHVTTKPSGSGMGLFLAHRIATTRYGGSLALEARVPLGTRAVLTAGPRGGGLRA"
     gene            1340..2707
                     /locus_tag="P5164_17590"
     CDS             1340..2707
                     /locus_tag="P5164_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011866138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRY45747.1"
                     /translation="MPEARVLLVEDEIDQRKIVAGILRGEGYSVAEAGSVDAALAELA
                     ASPVDLVLSDWKLPGRDGTELLAEVKARFPETGFVMATAYGTIAHAVEAVRAGADDYL
                     TKPFERAALLLAIERTLRARRLADENRRLSEEVRDRDRLVDLVGKSPSMARLYRQVEK
                     LAGTDATILLTGESGTGKELVARALHALSRRRAGAFVAVNAAAIPESLVEAEFFGSEK
                     GSFTGADRTRKGKLEQATGGTLFLDEVGELPLPLQPKLLRALQEGVVTRIGGAVEVPT
                     DARILAATNRDLAREVAEGRFREDLYYRLNVVSVTLPPLRERREDIPLLVRHFVARTE
                     RRYGIRVEPFPPPLLARLVDHSWPGNVRELANVVERLVLLSEGGRVSAGDLPEPLGGR
                     PAPDAGWKLPPGGLSWEEHEKDALRQAVELAGGNRARAARLLELPYKAFLYRLEKFGL
                     AAVEE"
     gene            2860..>3509
                     /locus_tag="P5164_17595"
     CDS             2860..>3509
                     /locus_tag="P5164_17595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45748.1"
                     /translation="MSISRNLSRAALGALTAGAVITLVAPLLSYERAAQAGDREEETT
                     ITIATSKDKEATVLKLDPMKPGETKTLTSEAGKPVLVTRTEDGYTLKIGEKELTVKTL
                     ADGEGPRVLLPGGKEVHVVKVGEGDGPTMVFTGDDEKKVVMKKHAFAYTIGEGADTPR
                     AADVLEKAAPKSLESLDPRTRGAVEQVLQELLEAGDVLAPRTRPMVWHAKEGEDGER"
BASE COUNT          506 a         1264 c         1311 g          428 t
ORIGIN      
        1 tggctcctcg tggaggcgct cacccgcgac ctgcgcgtcg aggtgacggc cgtcgcgacc
       61 tcggtcggaa aggacgtcgt gagagtcctc cagcgccgcg acggaaagct cctcgagctg
      121 ggcggcgacg cgggcgtccg gcggatcgtc gtccgcgacg aggctcatgt cgtgaccctg
      181 cctccgccct cggcggcgga ggcgccggcg aaagaccgga cggccggacc ggaggccggc
      241 ccgaaggagg ccgaggggcc gcgattcgtc gtcacgacgg tccggaccga agggagcggg
      301 acgacctcct cgacgaagac gtggacgacg aaaggcggga ccggcttcct cttcctggag
      361 gggccggtgg agaaggtgaa gatcccgatc ccgggaaccg gcgtgaagga ggcggtcggt
      421 cgcttccgga ctcggctcgt cctcggctcc ctcgggatcg tcgccttcgg cctcctggcg
      481 gccgcgttcg tcgcgcaccg cgtgacgcgc ccgctcgcgg agctctcccg cgccgcacga
      541 acggtcggcg aaggggcgtt cggcacgcgg gtcgagacgc gcgagggggg cgaggtgggg
      601 gcggcgatcg ccgcgttcaa ccagatgtcc tcgcaccttc tggccctcga gcgcgaggcg
      661 ctcgccctca aggagcgcga gcacctcggc gagatgggcg aggtcgcgcg cgggatggcg
      721 cacgcgctcc ggaacccgct ccacgccgtc ggcctctcgg tcgacgagct cgccgcacgc
      781 cttcccgacg accccgacgc cgccgcgatg gctgccgcgg cccggcgcca gatcgccggc
      841 gtcgacggga cgatccgctc gttcctcgcg ctcgccgccg gcggcggcgc cgaggaggag
      901 gtcgacgtcc gcgccctcgc agaggaggtg gccctcgcgg cgctccagga ggcgcgcgga
      961 cgggtccggg tgtcggtggc gccgggcggc gccgcgcgcc tcagggccgt cgcgccggag
     1021 ctgaaggcgg cgctccacgc cctcgtcgtc aacgccgtcg aggcgagccc cgacggcggc
     1081 gaggtcgtcg tctcggtcgc cggagaggaa gggcgcaccg tcgtccgcgt ggaggacgag
     1141 gggccgggtc tccccgagga ggtccgcgcc cgcctcttca cgccccacgt gacgacgaag
     1201 ccgagcggct ccgggatggg gctcttcctc gcgcaccgga tcgcgacgac gcgctatggc
     1261 gggagcctcg cgctcgaggc ccgggtcccg ctcgggacgc gggccgtcct gacggccggc
     1321 ccccgcggcg gagggctccg tgcctgaggc gcgggtcctc ctcgtcgagg acgagatcga
     1381 ccagcggaag atcgtggccg ggatcctgcg cggcgagggg tactccgtcg ccgaggcggg
     1441 ctccgtcgac gccgccctcg ccgagctcgc ggcctcgccc gtcgacctcg tcctctccga
     1501 ctggaagctc cccgggcgag acgggacgga gctcctcgcg gaggtgaagg cccgcttccc
     1561 ggagaccggg ttcgtgatgg cgacggccta cgggacgatc gcccacgcgg tcgaggcggt
     1621 gcgcgcggga gccgacgact acctgacgaa gccgttcgag cgcgcggccc tcctcctcgc
     1681 gatcgagcgg acgcttcgtg cgcggcgcct cgccgacgag aaccggcggc tctccgagga
     1741 ggtccgcgac cgcgaccgcc tcgtcgacct cgtcgggaag agcccgtcga tggcgcggct
     1801 ctaccggcag gtcgagaagc tcgcggggac cgacgcgacg atcctcctga cgggggagag
     1861 cggcacgggg aaggagctcg tcgcgcgggc tctccacgcc ctctcgcggc ggcgggcggg
     1921 ggccttcgtc gcggtgaacg ccgcggcgat cccggagagc ctcgtggaag ccgagttctt
     1981 cggctcggag aagggttcgt tcacgggtgc cgaccggacg cgaaaaggga agctcgagca
     2041 ggcgacgggc gggaccctct tcctcgacga ggtcggggag ctgccgctcc cgctccagcc
     2101 gaagctcctc cgcgcgctcc aggaaggcgt cgtgacgcgg atcggcgggg cggtcgaggt
     2161 cccgaccgat gcccgcatcc tggccgccac gaaccgcgac ctcgcgcggg aagtcgccga
     2221 ggggcgcttc cgcgaggacc tctactaccg gctgaacgtc gtctccgtga cgctccctcc
     2281 gcttcgcgaa aggcgcgagg acataccgct cctcgtccgc cacttcgtcg cccggacgga
     2341 gcggcgctac ggcatccgcg tcgagccgtt ccctccgccg ctcctcgcga ggctcgtcga
     2401 ccactcctgg cccgggaacg tccgcgagct cgcgaacgtc gtcgagcggc tcgtcctcct
     2461 ctccgagggg ggacgtgtct cggcggggga cctccccgag ccgctcggcg gacggcccgc
     2521 gcccgacgcg ggctggaagc tcccgccggg aggcctgtcg tgggaggagc acgaaaagga
     2581 cgccctccgg caggcggtcg agctcgcggg ggggaaccgg gcccgcgcgg cccgcctcct
     2641 cgaactcccc tacaaggcct tcctctaccg cctcgagaag ttcgggctcg ccgccgtcga
     2701 ggagtaggtc ccccgggcag gcgagcaccc gctttcggga gcggtttccc catttgggga
     2761 gccgaccccc ccgccaggcg ccccgatctg ctggcccggc gcttgcgcca ggacgaggaa
     2821 agccggcgcg aagccggaaa aagcgaacgg aggaccccga tgagcatctc tcgcaacctc
     2881 tcccgcgccg ccctcggcgc gctcaccgcc ggtgccgtga tcaccctcgt cgccccgctc
     2941 ctctcgtacg agagggccgc ccaggcgggc gacagagagg aagagacgac gatcacgatc
     3001 gcgacctcga aggacaagga ggcgaccgtc ctgaagctcg acccgatgaa gccgggcgag
     3061 acgaagacgc tgacctccga ggcgggcaag ccggtcctcg tgactcgtac cgaggacggc
     3121 tacacgctga agatcggcga aaaggagctc accgtgaaga ccctcgcgga cggcgagggg
     3181 cctcgcgtcc tcctgccggg cggcaaggag gtccacgtcg tgaaggtcgg cgaaggggac
     3241 ggtccgacga tggtcttcac gggcgacgac gagaagaagg tcgtcatgaa gaagcacgcc
     3301 ttcgcctaca cgatcggcga gggcgccgac acgccgcgag ccgcggacgt cctcgagaag
     3361 gccgccccga agagcctcga gagccttgac ccccgcacgc gcggcgccgt cgagcaggtc
     3421 ctccaggagc tgctcgaggc gggcgacgtc ctcgcgccgc gaacgagacc gatggtctgg
     3481 cacgcgaaag agggggaaga cggcgagcg
//
LOCUS       DAOTEZ010000449         3508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_20244_length_3508_cov_6.241819, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000449 DAOTEZ010000000
VERSION     DAOTEZ010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20244_length_3508_cov_6.241819"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="P5164_17600"
     CDS             <1..437
                     /locus_tag="P5164_17600"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45749.1"
                     /translation="LKEKRRRAVLDALLALNPVDAPETMVTAEVDSSLQQYARFLASQ
                     GMDLKEAKMDWNKLREEARPAAVRRVKEYLLLDAIAEAETLTVSDTELDADLKRRARS
                     MGAAFGELKSQLVKNDRLEGIREELRIDKALELLLTEAVAAS"
     gene            465..1049
                     /gene="clpP"
                     /locus_tag="P5164_17605"
     CDS             465..1049
                     /gene="clpP"
                     /locus_tag="P5164_17605"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HRY45750.1"
                     /translation="MLVPMVIEQTNRGERAYDIYSRLLKDNVIFLGTAINDEVANLVI
                     AQMLFLEAENPEKDITLYINSPGGSVTAGLAIYDTMQYVKPDVATYCIGQAASMGAVL
                     LAGGKKGKRAALPNARVLIHQPLAYGLEGQASDIEIHARDLVRMKKRLNEILSAHTGQ
                     PFERIEQDTDRDNIFEAEAAKEYGLIDNVLRNRD"
     gene            1120..2364
                     /gene="clpX"
                     /locus_tag="P5164_17610"
     CDS             1120..2364
                     /gene="clpX"
                     /locus_tag="P5164_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRY45751.1"
                     /translation="MTKKNGSGEVLRCSFCNKSQRDVKKLIAGPTVYICDECVDICLD
                     IIAEDRVLDQAKPESKLPKPREIKEFLDEYVVGQEQAKKKLAVAVYNHYKRIDYFERN
                     RKLDVELQKSNILLIGPTGTGKTLLAQTLAKMLSVPFTIADATTLTEAGYVGEDVENI
                     ILKLYQAAGQDKDKTERGIVYIDEIDKIARKGENPSITRDVSGEGVQQALLKILEGTV
                     TNVPPQGGRKHPHQEFIQIDTTNILFICGGAFVGLEDLIARRLTEKQMGFGAKIESKK
                     QKRSGATLEKVHPEDLIKFGMIPEFIGRVPVTATLHDLDKAALVAILKEPKNSLLKQY
                     QTLLEFENVGLRFTDDAVEEVADEAMKRTLGARGLKVILEELMLDVMYRVPSEEEIEE
                     CVITKDVVQRRAAPILSLRKVG"
     gene            2440..>3508
                     /locus_tag="P5164_17615"
     CDS             2440..>3508
                     /locus_tag="P5164_17615"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HRY45752.1"
                     /translation="MTNPATVPQRSATRTLPLVPLRDMVVFPRMMAPFVVGRASSIAA
                     LEAALLTSDKQIFLAAQRDPKIDEPAAGDIHDVGVVATVVQSLKLPNGHIKVMVEGVS
                     RGVIRSFATTGGHKEVEVAAAEARPLPGGAAELGPYMQKVLGVFEQYAKLSHQLAYEG
                     LLASLTLDDPDVFADMLAGHLPASIPTPEKQGLLETVNPLERLQKLQDLLEIEIEKIN
                     IDRRINNKVKKQMERAQKEYYLNEKIKAIHQELGRKDDRSDELEELRQKIDAAGMPKD
                     VKEKALAELKRLEAMPNVSAEATVSRNYIDWLVNVPWKKASRESKDIKNAEKVLNEDH
                     YGLDKVKERILEFLAVRQLVTE"
BASE COUNT          703 a         1185 c         1164 g          456 t
ORIGIN      
        1 cgctgaagga gaagcggcgc cgcgccgtcc tcgacgcgct cctcgccctg aaccctgtcg
       61 acgcgcccga gacgatggtg accgccgagg tcgattcctc tctccagcag tacgcgcgct
      121 tcctcgcctc ccaggggatg gacttgaagg aagcgaagat ggactggaac aagctccgcg
      181 aggaggcccg cccggcagcc gtccggcggg tgaaggagta cctcctcctc gacgcgatcg
      241 ccgaggccga gacgctcacc gtcagcgaca cggagctgga cgccgacctc aagcgcaggg
      301 cccgctcgat gggcgccgcc ttcggcgagc tcaagtcgca gctcgtgaag aacgaccgcc
      361 tcgaggggat ccgggaggag ctccggatcg acaaggccct cgagctgctc ctgacggaag
      421 ccgtcgcggc gtcgtgaccg tataaaaggg acaggaggag accgatgctg gtcccgatgg
      481 tcatcgagca gacgaaccgc ggggagcggg cgtacgacat ctactcccgc ctcctgaagg
      541 acaacgtcat cttcctcggc acggcgatca acgacgaggt ggcgaacctc gtgatcgccc
      601 agatgctctt cctcgaggcg gagaaccccg agaaggacat cacgctctac atcaacagcc
      661 ccggcgggtc ggtgacggcc ggcctcgcga tctacgacac gatgcagtac gtcaagcccg
      721 acgtcgcgac ctattgcatc gggcaggcgg cctcgatggg ggcggtcctc ctcgccggcg
      781 ggaagaaggg gaagcgggcg gcgctcccga acgcgcgcgt cctgatccac cagccgctcg
      841 cctacggcct cgaggggcag gcgtccgaca tcgagatcca cgcccgcgac ctcgtccgga
      901 tgaagaagcg gctgaacgag atcctctcgg cccacacggg ccagccgttc gagcggatcg
      961 agcaggacac ggaccgcgac aacatcttcg aggccgaggc cgcgaaggag tacgggctca
     1021 tcgacaacgt gctccggaac cgggactgac cccggctccc gagacggccc ccggagagcc
     1081 gggggcgcta gaattccccc ggaaagggtc tattcaggga tgacgaagaa gaacggctcc
     1141 ggcgaggtcc tccgttgcag cttctgcaac aagtcgcagc gcgacgtgaa gaagctgatc
     1201 gcggggccga cggtctacat ctgcgacgag tgcgtcgaca tctgcctcga catcatcgcg
     1261 gaggaccgcg tcctcgacca ggccaagccc gagtcgaagc tcccgaagcc gcgcgagatc
     1321 aaggagttcc tcgacgagta cgtcgtcggc caggagcagg cgaagaagaa gctcgccgtc
     1381 gcggtctaca accactacaa gcggatcgac tacttcgagc ggaaccgcaa gctcgacgtc
     1441 gagctccaga agtcgaacat cctcctgatc ggcccgacgg gcacgggcaa gacgctcctc
     1501 gcccagacgc tcgcgaagat gctctcggtg ccgttcacga tcgccgacgc gacgacgctc
     1561 accgaggcgg gctacgtcgg cgaggacgtc gagaacatca tcctgaagct ctaccaggcg
     1621 gcggggcagg acaaggacaa gaccgagcgc ggcatcgtct acatcgacga gatcgacaag
     1681 atcgcccgca agggggagaa cccgtccatc acgcgcgacg tctccggcga gggggtccag
     1741 caggcgctgc tgaagatcct cgaggggacg gtcacgaacg tcccgccgca gggcggccgc
     1801 aagcacccgc accaggagtt catccagatc gacacgacga acatcctctt catctgcggg
     1861 ggggcgttcg tcggcctcga ggacctcatc gcccgccggc tgaccgagaa gcagatgggc
     1921 ttcggcgcga agatcgagtc gaagaagcag aagcgctcgg gggccacgct cgagaaggtc
     1981 cacccggagg acctgatcaa gttcgggatg atccccgagt tcatcggccg cgtcccggtg
     2041 acggcgacgc tccacgacct cgacaaggcg gccctcgtcg ccatcctgaa ggagccgaag
     2101 aactccctcc tgaagcagta ccagaccctc ctcgagttcg agaacgtcgg cctccggttc
     2161 accgacgacg ccgtggagga ggtcgccgac gaggcgatga agcgcacgct cggcgcgcgc
     2221 ggcctcaagg tgatcctcga ggagctcatg ctcgacgtga tgtaccgcgt cccctccgag
     2281 gaggagatcg aggagtgcgt catcacgaag gacgtcgtcc agcggcgcgc cgcgccgatc
     2341 ctgtcgctcc ggaaggtcgg ttgacggccg tccccggacg ggcgagcggc cccgcgcccc
     2401 gcgcgcgagg ccgaagcccc cagccacgaa ggtgaccgca tgacgaaccc cgcgacagtg
     2461 ccccaaaggt ccgcgacgcg gacgctgccg ctcgtgcccc tccgggacat ggtcgtcttc
     2521 ccccggatga tggccccctt cgtcgtcggc cgcgcctcgt cgatcgcggc gctcgaggcg
     2581 gcgctcctga cgagcgacaa gcagatcttc ctcgccgccc agcgcgaccc gaagatcgac
     2641 gagcccgccg cgggcgacat ccacgacgtc ggcgtcgtgg cgacggtcgt ccagtcgctc
     2701 aagctcccga acggccacat caaggtgatg gtggaggggg tctcgcgcgg cgtcatccgc
     2761 tccttcgcga cgacgggcgg gcacaaggag gtcgaggtcg ccgcggcgga ggcgaggccg
     2821 ctcccgggcg gcgcggccga gctcggcccg tacatgcaga aggtgctcgg cgtcttcgag
     2881 cagtacgcca agctctcgca ccagctcgcc tacgaggggc tcctcgcctc gctcacgctc
     2941 gacgaccccg acgtcttcgc cgacatgctc gcgggccacc tcccggcgtc gatcccgacg
     3001 cccgagaagc aggggctcct cgagacggtg aaccccctgg aacggcttca gaagcttcag
     3061 gacctgctgg agatcgagat cgagaagatc aacatcgacc ggcgcatcaa caacaaggtc
     3121 aagaagcaga tggagcgggc ccagaaggag tactacctca acgagaagat caaggcgatc
     3181 caccaggagc tcgggcggaa ggacgaccgc tccgacgagc tggaggagct gcggcagaag
     3241 atcgacgccg cggggatgcc gaaggacgtc aaggagaagg ccctcgccga gctgaagagg
     3301 ctcgaggcga tgccgaacgt ctcggccgag gcgacggtct cgcgcaacta catcgactgg
     3361 ctcgtgaacg tcccctggaa gaaggcctcg cgcgagtcga aggacatcaa gaacgccgag
     3421 aaggtcctga acgaggacca ctacgggctc gacaaggtga aggagcggat cctcgagttc
     3481 ctcgcggtcc gccagctcgt caccgaga
//
LOCUS       DAOTEZ010000450         3508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_20245_length_3508_cov_5.514625, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000450 DAOTEZ010000000
VERSION     DAOTEZ010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20245_length_3508_cov_5.514625"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="P5164_17620"
     CDS             <1..1102
                     /locus_tag="P5164_17620"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF025376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HRY45753.1"
                     /translation="PDHPGVIRQFARADGVTMQGIRADDPAAAQRLAAAFRAAVASPA
                     PSPAMTAADRDALGKLLESGVKAHTETLLRIAPLLAPFGGLVPQTRVRIRATGSVNYG
                     RSIPSYPEEWGGGTTLPDNRDLRDAWPEGVTLPRAIVYNLGGTTLGLPAVTSDLAVLD
                     RRGAVLLERHVPGYREIVATELPAFVKEAAALVFGKKLAETTARRCLRAAAALWVDAR
                     TREDFVFPTCLFAMEYLRWLAEDSESWDETKEHESRTTRAEELIRETSSAAFSALCAE
                     HPGDRWPVLQGLVDAEDATRLLLARELTLEEKREFVDLRIEGWLRALPEPLARELRRE
                     WARLRELGKDDLELDAIERLVALEELRGSRGA"
     gene            1308..2255
                     /locus_tag="P5164_17625"
     CDS             1308..2255
                     /locus_tag="P5164_17625"
                     /inference="COORDINATES: protein motif:HMM:NF039296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5924 family protein"
                     /protein_id="HRY45754.1"
                     /translation="MIVGRDRPELLRWGLASIGAVWGSSLALPAFLSARWLAAERKEP
                     ARRLVLWAQKWLLQGLAFFLLPLYHRSAAYTPGQTAFMALLAAAALAATIDVVYDDVV
                     TPRRPLHGTLLAFITFATVNVALPMVWRTGGLWSLGASGLLATAVFVSFVASSGSDRG
                     LGRPAVVGALFLALVTLGRPAVPPAPLRLVSAHVGRSLAPNGLDVASPLPSLPAGTGG
                     PLVAVAAVFAPAGLDEGVRHVWSLNGRPLLRSRVIGITGGRKDGFRSRSGVTVGRLAA
                     GQRLRLDVETAHGQLVGRVELVVTGAAPRDRTSSGAQPL"
     gene            2266..2721
                     /locus_tag="P5164_17630"
     CDS             2266..2721
                     /locus_tag="P5164_17630"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRY45755.1"
                     /translation="MRSVAREIVLNASPDRVFTAFTDVPEVLAWLADAAVIGPRVGGN
                     WALGWYADEDSDDGYHVFGTFEVFEPSSRLVVRDLRFSTPEGELLEGMRLSIEIGAEN
                     GGTRVSLRHEGLGEGASWDGYTAGLGPSWERSLADLRGWLEEGRKLPGR"
     gene            2869..3210
                     /locus_tag="P5164_17635"
     CDS             2869..3210
                     /locus_tag="P5164_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007955303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRY45756.1"
                     /translation="MGDGAWVKELKVGVTVCDRDGIALEMNDRAAETFAADGGRDLVG
                     KSILDCHPPRARALFEELLRAGGTNAYTIEKNGVKKLIYQAPWYEDGVYRGIVELSLP
                     IPFDLPHFVRG"
     gene            complement(3285..>3508)
                     /locus_tag="P5164_17640"
     CDS             complement(3285..>3508)
                     /locus_tag="P5164_17640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45757.1"
                     /translation="VRKIDGRLTTPVRVLGGALLEVKGVRLLYETREVLPGLWAPCRE
                     EISITATAAMVLPYRRELRFEFGRYRPAT"
BASE COUNT          445 a         1313 c         1250 g          500 t
ORIGIN      
        1 ccccgaccac ccgggcgtga tccggcagtt cgcgcgggct gacggcgtca cgatgcaggg
       61 gatccgggcc gacgacccgg ccgccgcgca gcgcctcgcc gccgccttcc gagctgccgt
      121 cgcctcgccg gctccgtcgc ccgcgatgac cgccgcggac cgcgacgccc tcgggaagct
      181 cctcgagtcc ggcgtgaagg cccacaccga gacgctcctg cgcatcgcgc cgctcctcgc
      241 gccgttcggc ggcctcgtgc cgcagacgcg cgtgaggatc cgcgcgaccg gctccgtgaa
      301 ctacgggcgt tcgatcccgt cctaccccga ggagtggggc ggcgggacga cgctccccga
      361 caaccgcgac ctgcgcgacg cctggcccga gggggtcacc ctcccgcgcg ccatcgtcta
      421 caacctcggc ggcacgacgc tcggcctccc cgccgtcacg agcgacctcg ccgtcctcga
      481 ccggcgcggg gccgtcctcc tcgagcggca cgtccccggc taccgcgaga tcgtcgccac
      541 cgagctcccc gccttcgtga aggaggccgc ggccctggtg ttcgggaaga agctcgccga
      601 gacgaccgcg cgccggtgcc tgcgcgccgc ggcggcgctc tgggtcgacg cgcggacacg
      661 ggaggacttc gtcttcccga cctgcctctt cgcgatggag tacctccgct ggctcgccga
      721 ggactcggag agctgggacg agacgaagga gcacgagagc cggacgacgc gcgcggagga
      781 gctgatccgc gagacctcga gcgccgcctt ctccgcgctc tgcgccgagc atccgggcga
      841 ccgctggccg gtcctccagg ggctcgtcga cgccgaggac gcgacgcggc tcctcctcgc
      901 ccgcgagctc acgctggagg agaagaggga gttcgtcgac ctgcggatcg aggggtggct
      961 ccgcgcgctt cccgagcccc tcgctcgcga gcttcgccgc gagtgggctc gcctgcgcga
     1021 gctcggcaag gacgacctcg agctcgacgc gatcgagcgg ctcgtcgcgc tcgaggagct
     1081 gcgcgggagc cggggagcct gagctcgcgc caggtgccgc ctccggcccg cgcaggcgga
     1141 aggcgggtat cctctcgcgc ctgcggtgcg ccgcggggga gggaggcgga gacctgcgcc
     1201 gtcgctggga agagaggatc gggacgttcg cccgcttcgc ggcgcgccat caggccctgt
     1261 tctggacgct gcactccctc tgggccctcg gctggggagt cgcgttcgtg atcgtggggc
     1321 gggaccgccc cgagctgctt cgctgggggc tcgcctcgat cggagcggtc tggggaagca
     1381 gcctcgcgct ccccgcgttc ctctccgcgc ggtggctcgc cgcggagcgg aaggagccgg
     1441 cgcgccggct cgtcctctgg gcccagaagt ggctcctgca ggggctcgcg ttcttcctcc
     1501 ttcctctgta tcaccggagc gcggcctaca cgccggggca gacggccttc atggcgctcc
     1561 tcgccgcggc cgctctcgcg gcgaccatcg acgtcgtcta cgacgatgtc gtaacgcccc
     1621 ggcgtccctt gcacgggacg ctcctcgcgt tcatcacgtt cgcgaccgtc aacgtcgctc
     1681 ttccgatggt gtggcgcacg ggcggcctct ggagcctcgg ggcgagcggc ctcctcgcca
     1741 cggccgtctt cgtctcgttc gtcgcctcct ccggatcgga ccgggggctc ggccgcccgg
     1801 cggtcgtcgg ggccctcttc ctcgccctcg tcaccctcgg acgcccggcc gtcccgcccg
     1861 ccccgctgcg gctcgtgagc gcgcacgtcg gccgctcgct cgccccgaac ggcctcgacg
     1921 tcgcctcgcc gctcccgtcg ctccccgcag gaaccggcgg gcctctcgtc gccgtcgcgg
     1981 ccgtcttcgc ccccgcgggg ctcgacgaag gggtccggca cgtctggtcg ctgaacggcc
     2041 ggcccctcct ccgatcgcgc gtcatcggga tcaccggcgg ccggaaggac gggttccgct
     2101 cccgttccgg cgtgaccgtg ggccggctcg ccgccgggca gcggctccgg ctcgacgtcg
     2161 agacggcgca cggacagctc gtcggcaggg tcgagctcgt cgtgacgggc gcggctccgc
     2221 gagatcggac gtcgagcgga gcgcagcctc tatgatccgg cgtcgatgag atccgtcgct
     2281 cgcgagatcg tgctgaacgc gtcgcccgat cgcgtcttca cggcattcac cgacgtgccg
     2341 gaggtcctcg cgtggctcgc cgacgcggcg gtcatcggtc cgcgcgtcgg cgggaactgg
     2401 gccctcggct ggtacgccga cgaggactcc gacgacggtt accacgtctt cggcacgttc
     2461 gaggtcttcg agccgtccag ccgcctcgtc gtgagggacc tccgcttctc cactccggag
     2521 ggagagctgc tcgaggggat gcgtctctcg atcgagatcg gcgccgagaa cggcggaaca
     2581 cgcgtctcac tgcggcacga gggcctcggc gaaggagcct cctgggacgg ctacaccgcc
     2641 gggctcggac cgagctggga gcgctccctc gcggatctcc gcggctggct cgaggagggg
     2701 cggaagctcc ccggacgctg accggacccg gtccgcttcg agccgggatt ccccccttcg
     2761 agccgaaccc gcccatcggc ccgatcctgc agggctgaag gacgcctcgc gattccgtac
     2821 catccctcct tgcgccccgc ggcgggacgc gaggagagag gaggcgccat gggcgacgga
     2881 gcctgggtga aggagctgaa ggtcggggtc accgtctgcg accgcgacgg aatcgccctc
     2941 gagatgaacg accgggcggc cgagacgttc gccgcggacg gcgggcgcga cctggtcggg
     3001 aagagcatcc tcgactgcca tccgccgaga gcacgggcgc ttttcgagga gctcctccgc
     3061 gccggcggga cgaacgccta cacgatcgag aagaacgggg tgaagaagct catctaccag
     3121 gccccctggt acgaggacgg cgtctaccgc ggaatcgtcg agctctcgct cccgatcccg
     3181 ttcgacctcc cccacttcgt ccgaggctga gatccggggg ggtctggtct gcacccgacg
     3241 ctctgctcgg gagcagagcg tcgggtgcag accagacccc aggctcaggt ggcggggcgg
     3301 tagcggccga actcgaagcg gagctcgcgg cggtaaggga ggaccatcgc ggcggtcgcc
     3361 gtgatcgaga tctcctcgcg gcagggggcc cagagacccg gaaggacctc gcgcgtctcg
     3421 tagaggaggc gcacgccctt cacctcgaga agggccccgc cgaggacccg gaccggcgtc
     3481 gtcagccgcc cgtcgatctt ccgcacct
//
LOCUS       DAOTEZ010000451         3498 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_20328_length_3498_cov_7.309323, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000451 DAOTEZ010000000
VERSION     DAOTEZ010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20328_length_3498_cov_7.309323"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="P5164_17645"
     CDS             complement(<1..1109)
                     /locus_tag="P5164_17645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HRY45758.1"
                     /translation="MAKTICLLQYVQSVPRTAENIAACLHASIDADSCLAPVREALVA
                     LEKAHLVRQGDDGYRIPTPAEDDWEKTRNGLSPRPGDVHRLHSETVSAFWKPQPSYTY
                     LETKAFKAGLAIHGRADTDGDVVFHLHFAEDGQAFERLAAELRQRSREERRNVFWAVA
                     LNEAIDRETVELYRSKEMLDRKERDARTADQSALVGEEKRRLKRYQDELRRLLRAACL
                     SGSVSFRGNDRSPRDGSDVNKAAAEILGATLPELFERFGEAAARTAELKRALDALLAA
                     TSLEGLPGTFHALHLLRDEKGKTLFHLDDGPLAEVLRRIEERSGYGDTTSGRWLADEF
                     EKEPFGWDFEAVRLFALCLLRAGKVEMTSAGQTIDS"
     assembly_gap    1146..1245
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1246..2363)
                     /gene="brxC"
                     /locus_tag="P5164_17650"
     CDS             complement(<1246..2363)
                     /gene="brxC"
                     /locus_tag="P5164_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HRY45759.1"
                     /translation="MTNAAIESLFASNIRRQIEEVIKVDQTDETILREEIDEYVVTDA
                     IRKHYTEVLDRYSETPGKPHEGIGVWVSGFFGSGKSSFAKILGLALSNRPIAGGHAGR
                     EFGERTGDTKLKVLLATIAEKVPTHAVIFDVSTDRGIRSGNQTLTEIMYGLFLQSLGY
                     AKDLDLSELEIALEEKGELAKFESEYARIYKKSWSAEKGKVAFALSQASNVLHELDSV
                     TFPMADSWVKAVKDKADITPGKLAERATELMKRRKPGYSLVFVVDEVGQFVARDVQKM
                     LDLQAIVQNLGRVGRGRHWVVVTSQEKLDQIVSGLDDKKIELARLMDRFPLQVHLEPS
                     DITEVTSRRVLAKNAAAQTALGKRFDEYRSRLAACTRVS"
     gene            complement(2394..2969)
                     /locus_tag="P5164_17655"
     CDS             complement(2394..2969)
                     /locus_tag="P5164_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HRY45760.1"
                     /translation="MSDLEQRIKTVLEPILELPDPRTRLSAYHDMPYAIFRYDPEEEL
                     EMRRLVSLLATRLAQKGKRVSRISLAECLDEALRSQNPLEDWIRAEREQGPSTLVDTI
                     HEVLSSYAPLVDLVASRMPAEPDPLRDVVFLLRTGALFPVYRTFSLLEQLKGRVRVPS
                     ILFYPGTLDGAAGLRFMGVLDAEHNYRPKIL"
     gene            complement(2966..>3498)
                     /locus_tag="P5164_17660"
     CDS             complement(2966..>3498)
                     /locus_tag="P5164_17660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HRY45761.1"
                     /translation="WGLDEWKPALLWHMTRDEFLLRDFLVGWLYPNWAAGAHRLRPEE
                     LYDFLREVGARGGVTEHAWGEATMERVAVGLLRMAADFGLLKGTAVREFASYHLPERS
                     FLYLLHAFRDTEPNPRRLIESPEWRMFLMEPADVERELLRLHQFQKLHYDVAGSLSQL
                     TLPCASALDYAGRMAA"
BASE COUNT          504 a         1063 c         1242 g          589 t
ORIGIN      
        1 gagtcgatcg tctgcccggc gctcgtcatc tcgaccttgc cggccctgag gaggcagagg
       61 gcgaagagcc ggacggcctc gaagtcccac ccgaagggct ccttctcgaa ctcgtccgcc
      121 agccagcgcc cgctcgtcgt gtcgccgtac ccggaccgct cctcgatgcg gcggaggacc
      181 tcggcgaggg ggccgtcgtc gaggtggaag agcgtcttcc ccttctcgtc ccggaggagg
      241 tggagggcgt ggaaggtccc gggcaggccc tcgaggctcg tggcggcgag gagggcgtcg
      301 agtgccctct tcagctcggc cgtccgggcg gcggcctcgc cgaaacgctc gaagagctcg
      361 ggcagagtgg ccccgaggat ctcggcggct gccttgttca cgtctgaccc gtctcgcggg
      421 cttcgatcgt ttccgcggaa gctgacgctg cccgagaggc acgcagccct gaggagccgg
      481 cgaagctcgt cctggtagcg cttcaggcgg cgcttctcct ccccgacgag ggccgactgg
      541 tcggccgtcc gggcgtcccg ctctttcctg tcgagcatct ccttcgaccg gtagagctcc
      601 acggtctccc ggtcgatggc ctcgttcaga gcgacggccc agaagacgtt ccggcgctcc
      661 tcccggctcc tctggcgaag ctcggcggcg agcctctcga aggcctgtcc gtcctcggcg
      721 aagtgaaggt ggaaaacgac gtcgccgtcg gtatcggccc gaccgtgaat tgccaggccg
      781 gccttgaagg ccttcgtctc gagataggtg taggacggct gaggtttcca gaatgcggag
      841 accgtctccg agtggagccg gtggacgtcg ccgggtcggg ggctgaggcc gttccgggtc
      901 ttctcccagt cgtcctcggc cggcgtgggg attcggtagc cgtcgtctcc ctgccggacg
      961 aggtgggcct tctcgagggc gacgagcgcc tcgcggacgg gggcgaggca ggagtccgcg
     1021 tcgatcgacg cgtgaaggca ggcggcgatg ttctcggcgg tccttggaac gctctggacg
     1081 tactggagga ggcagatggt cttggccacg gcctgcgcga ggggctggtc gaccctctgc
     1141 gggatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngagac gcgcgtgcag
     1261 gcggcgaggc ggctgcggta ttcgtcgaaa cgcttcccga gagccgtctg ggcggcggcg
     1321 ttcttcgcca ggacccggcg gctcgtgacc tcggtgatgt cggaaggctc gaggtggacc
     1381 tggagcggga agcggtccat caggcgggcc agctcgatct tcttgtcgtc gaggccggag
     1441 acgatctgat cgagcttctc ctgcgaggtg acgacgaccc agtgcctgcc gcggccgacg
     1501 cggccgaggt tctggacgat ggcctggagg tcgagcatct tctggacgtc gcgggccacg
     1561 aactgcccca cctcgtcgac gacgaagacg agggagtagc ccggcttgcg gcgcttcatc
     1621 agctcggtcg ctcgctcggc gagcttgccg ggggtgatgt cggccttgtc cttgacggcc
     1681 ttcacccacg agtcagccat cgggaaagtg accgagtcga gctcgtggag gacgttgctc
     1741 gcctggctca gggcaaaggc gaccttcccc ttctcggcgc tccagctctt cttgtagatg
     1801 cgggcgtact cgctctcgaa cttcgcgagc tcccccttct cctcgagcgc gatctcgagc
     1861 tcggagaggt ccaggtcctt cgcgtagccg aggctctgga ggaagagccc gtacatgatc
     1921 tcggtgaggg tctggttgcc gctccggatg ccgcggtcgg tggagacgtc gaagatgacg
     1981 gcatgggtgg ggaccttctc ggcgatcgtg gcgaggagga ccttcagctt cgtgtcgccg
     2041 gtgcgttctc cgaactccct gccggcatgc ccccccgcga tcggacggtt cgagagggcg
     2101 aggccgagga tcttcgcgaa actcgacttg ccggacccga agaagccgga gacccacacg
     2161 ccgattccct cgtgaggctt gcccggggtc tcggagtacc ggtcgaggac ttcggtgtag
     2221 tgcttccgga tggcgtcggt gacgacgtac tcgtcgatct cctcccggag gatcgtctcg
     2281 tcggtctggt cgaccttgat gacctcttcg atctggcgcc ggatgttgct cgcaaacagg
     2341 gactcgatag cggcgttcgt catagctggg gccttttcat ctcgggggcc tcttcagagg
     2401 atcttcggtc ggtagttgtg ctcggcgtcg aggacgccca tgaagcgaag gccggcagcg
     2461 ccgtcgagcg tccccgggta gaagaggatc gacgggacgc gtacgcggcc cttgagctgc
     2521 tcgaggagcg agaaggtgcg gtagacggga aaaagggcgc ccgtccggag aaggaagacg
     2581 acgtcgcgga gcgggtcggg ctcggccggc atccgcgagg cgacgaggtc gacgagcggg
     2641 gcgtacgagg agaggacttc gtggatggtg tcgacgagcg tcgagggccc ctgctcgcgc
     2701 tcggcacgga tccagtcttc gagcgggttc tgcgagcgaa gggcctcgtc gaggcactcg
     2761 gcgagggaaa tccgcgagac gcgcttcccc ttctgggcga gacgggtcgc caggaggctc
     2821 accaggcggc gcatctcgag ctcctcttcc gggtcgtacc ggaagatcgc gtacggcatg
     2881 tcgtggtagg cgctgaggcg ggtccgcggg tccgggagtt ccaggatcgg ctcgaggacg
     2941 gtcttgatgc gctgttcgag gtcgctcatg cggccatcct cccggcgtag tcgagggcgc
     3001 tcgcacacgg aagcgtcagc tgcgagaggc tcccggcgac gtcgtagtgc agcttctgga
     3061 actggtgcag ccggaggagc tcccgctcca cgtcggcggg ctccatgagg aacatccgcc
     3121 actccggcga ctcgatgagg cggcgagggt tcggctcggt gtcgcgaaat gcgtgaagga
     3181 ggtagaggaa gctccgctcc ggaaggtggt acgaggcgaa ttcccggacg gccgtgccct
     3241 tcaggaggcc gaagtcggcg gccatgcgga ggaggccaac ggcgacccgc tccatagtgg
     3301 cctctcccca ggcatgctcc gtgacgcctc cccgggctcc gacctcccgg aggaagtcgt
     3361 agagctcctc gggccggagc cgatgagccc ccgcggccca gttcgggtag agccagccga
     3421 cgaggaagtc ccggaggagg aactcgtcgc gggtcatgtg ccagaggagc gccggcttcc
     3481 actcgtcgag gccccagc
//
LOCUS       DAOTEZ010000452         3480 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_20490_length_3480_cov_5.723504, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000452 DAOTEZ010000000
VERSION     DAOTEZ010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20490_length_3480_cov_5.723504"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="P5164_17665"
     CDS             complement(<1..495)
                     /locus_tag="P5164_17665"
                     /inference="COORDINATES: protein motif:HMM:NF017409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HRY45762.1"
                     /translation="MSKPFSFGAIDVRLESGAEPRPARAEDDAPFRILLAGDFSGRGA
                     RGLVEAGAALGERKVYRVDLDSLDATIARLEPQVRLRDGDVLRFRELDDFLPDTLFSR
                     VARFGALKDAAAAESRRREAARPAAGLLDRILDGAPQDEAPLAEAGGKGLAAKMDSFK
                     VMIDR"
     gene            579..1559
                     /locus_tag="P5164_17670"
     CDS             579..1559
                     /locus_tag="P5164_17670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY45763.1"
                     /translation="MIRFLKRVGRRLPLLKEIHRYILALEAERDELRRTVATQREELV
                     RARETTRKLWVPPGDPRSPIPDLDDLRRRESELFGRPPDELPGIDLLVEPQLTFVREM
                     KRWGADPSLPPPGEGRRSDADDPAYGRADVALLHAFLRKLRPRRVVCAGAGAFVPSLL
                     DLNEHVCGRSIAITVVDPDAERLRPLLGPEDSEKVRLVPSRVHEVPYDTFTSLGPSDV
                     LCVETSHVSKTGSDLNHLLFRVLPRLKAGVHVHVDGVFWPFEYPSAWVTEGRAFNEAY
                     LWRAFLLNSSAWEIVLFASFLEASHRGALLKELPQWERSRGGGLWLRRKG"
     gene            complement(1618..2394)
                     /locus_tag="P5164_17675"
     CDS             complement(1618..2394)
                     /locus_tag="P5164_17675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45764.1"
                     /translation="MPTISTRKFIALRKLTRALAETLRADVRSTLVTIEPLLRPRAVF
                     GEHVQGHGKELVKGADRAFKDLAADFAEVAGSKPFHLPKELKSPFEVMSASLEMQPVE
                     YSYTARGEREGKTVRITSPLKWVVNYRDYGLDELKRVLADRNRTEEQLRRFVLHFLLV
                     EHVFTRQAGIREMLDALRFPVVVERRPEFGNLPITCVTTAVSTYRPDDDVIIETTEIS
                     GSEEFEEFVKVSDVSGMKDALKEKLVELVRTQGPELLDEA"
     gene            complement(2495..3253)
                     /locus_tag="P5164_17680"
     CDS             complement(2495..3253)
                     /locus_tag="P5164_17680"
                     /inference="COORDINATES: protein motif:HMM:NF015709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HRY45765.1"
                     /translation="MSKVPIAVASAALAFALTSCAPAMRPPAAAPAETAVRPNRPTDE
                     LHWVRASAEYRAVTIQTFRAALEAAEEASAGRPAGSWAVCVDADDTIIDNSGYEAELQ
                     AAGRTHTDEAWFAWVKRRARPAVPGAATFLAGIRALGGRVAVVTNTQASLCDDVAANL
                     VLAGLPYDVLVCRPDDAGDRKEPRFRLVTEGAARPDLGPLEILVWVGDNIQDFPEQSQ
                     KLRDEPESAFGDFGVRYFALPNPIYGTWEKNGPR"
BASE COUNT          464 a         1243 c         1258 g          515 t
ORIGIN      
        1 gcggtcgatc atcactttga agctgtccat cttggccgcc agacccttgc cgccggcctc
       61 ggcgagcggc gcctcgtcct gcggcgctcc gtcgaggatc cggtcgagga ggcccgcggc
      121 cgggcgcgcc gcctcccgcc ggcgcgactc tgccgcggcg gcgtccttca gcgcgccgaa
      181 gcgcgcgacg cgcgagaaga gcgtgtccgg gaggaagtcg tccagctctc ggaagcggag
      241 gacgtctccg tccctgagcc gtacctgcgg ctcgagccgg gcgatcgtcg cgtcgagcga
      301 gtcgaggtcg acgcggtaca ccttcctctc tccgagcgcg gcgcccgcct cgacgagacc
      361 gcgcgctccc cgtcccgaga agtccccggc gaggaggatc cggaacggag cgtcgtcctc
      421 cgcgcgcgcg gggcggggct cggcgccgga ttcgagacgg acgtcgatcg caccgaagga
      481 gaacggcttc gacatcggcg gggacctcct cgacctttgg tgggctcgag tataggggcg
      541 acgcgctaga ctgggcccct cgccgcaagg aggtctcgtt gatccgcttc ctgaagaggg
      601 tggggcggcg gctgccgttg ctgaaggaga tccaccgcta catcctcgcg ctcgaggcgg
      661 agcgggacga gctccggcgg accgtcgcga cgcagcgcga ggagctcgtc cgggcgcggg
      721 agacgacgcg caagctctgg gtaccgcccg gcgacccgcg ctcgccgatc cccgacctcg
      781 acgacctgcg ccggcgcgag agcgagctct tcggccgccc gcccgacgag ctgccgggga
      841 tcgacctgct cgtcgagccg cagctgacgt tcgtccgcga gatgaagcgg tggggagccg
      901 acccgtccct cccgccgccg ggcgagggga ggaggagcga cgccgacgac cccgcctacg
      961 gccgggccga cgtggcgctc cttcacgcgt tcctccggaa gctgcgcccc cggcgcgtcg
     1021 tctgcgcggg ggccggggcc ttcgtccctt cgctcctcga tctgaacgag cacgtctgcg
     1081 gacgttcgat cgcgatcacc gtcgtcgacc ccgacgccga gcgcctccgc ccgctcctcg
     1141 gcccggagga ctcggagaag gtccggctcg tcccctcccg cgtccacgag gtcccctacg
     1201 acaccttcac ctcgctcggc ccgagcgacg tcctctgcgt cgagacgagc cacgtgtcga
     1261 agacggggag cgacctgaac cacctcctct tccgcgtcct gcctcggctg aaggcggggg
     1321 tgcacgtcca cgtcgacggc gtcttctggc ccttcgagta cccgagcgcg tgggtgacgg
     1381 aggggcgggc cttcaacgag gcgtacctct ggcgcgcgtt cctcctcaac agctccgcgt
     1441 gggagatcgt cctcttcgcg agcttcctcg aggcgagcca ccggggagcc ctcctgaagg
     1501 agctcccgca gtgggagagg agccgcggag gaggtctctg gctgcgccgg aagggctgac
     1561 cggcgggggg agcccgccgg cggaggaggc gccccgcccg ccggccggcc tggtgcgtca
     1621 ggcctcgtcc aggagctccg gcccctgcgt ccggacgagc tcgacgagct tctccttcag
     1681 ggcgtccttc atccccgaga cgtcggagac cttcacgaac tcctcgaact cctcgctccc
     1741 ggagatctcc gtcgtctcga tgatcacgtc gtcgtcgggg cggtaggtcg agacggccgt
     1801 cgtgacgcag gtgatcggga ggttcccgaa ctcggggcgc cgttccacga cgaccgggaa
     1861 gcggagcgcg tcgagcatct cccggatccc cgcctgccgt gtgaagacgt gctcgacgag
     1921 gaggaagtgg aggacgaagc gccgcagctg ctcctcggtc cggttgcggt cggcgaggac
     1981 gcgcttgagc tcgtcgagcc cgtagtcgcg gtagttcacg acccacttca gaggcgaggt
     2041 gatccggacg gtcttgccct cgcgctctcc gcgggcggtg taggagtact cgaccggctg
     2101 catctcgagg gaggcgctca tcacctcgaa cggcgacttc agctccttgg gaaggtggaa
     2161 cggcttgctt ccggcgacct ccgcgaagtc ggccgcgagg tccttgaagg cccggtcggc
     2221 gcccttcacg agctccttcc cgtgcccctg tacgtgctcg ccgaagaccg cccgcggacg
     2281 gagcagcggc tcgatcgtga cgagcgtgct gcgcacgtcg gcgcggagcg tctccgccag
     2341 ggcgcgggtc agcttacgaa gggcgatgaa cttccgcgtg ctgatcgtcg gcattcgcgt
     2401 cccctctccc ggcgcgccgg gccgcgccgc ggctctccgg atggtcgttt ccggcgagtc
     2461 tggagccgat tcgacggggc gtcaaaccgc ccgctcagcg ggggccgttc ttctcccagg
     2521 ttccgtagat cgggttcggc agggcgaagt accggacccc gaagtcgccg aacgcgctct
     2581 cgggctcgtc gcgcagcttc tggctctgct cggggaagtc ctggatgttg tcgccgaccc
     2641 agacgaggat ctcgagcggc cccaggtcgg gccgcgcggc cccctcggtg acgagccgga
     2701 agcgaggctc cttccggtcc cccgcgtcgt ccggccggca gaccaggacg tcgtacggca
     2761 ggcccgcgag gacgaggttc gccgccacgt cgtcacagag ggacgcctgg gtgttcgtca
     2821 cgacggcgac ccggccgccg agcgcgcgga tcccggcgag gaaggtggcg gcgccgggga
     2881 cggccggcct cgcgcgcctc ttcacccagg cgaaccacgc ctcgtcggtg tgcgtgcggc
     2941 ccgccgcctg cagctcggcc tcgtagccgg agttgtcgat gatcgtgtcg tcggcgtcga
     3001 cgcagacggc ccagctcccg gcggggcggc ccgcgctggc ctcttcggcg gcctccagcg
     3061 cggcccggaa ggtctggatc gtcacggccc ggtactcggc cgaggcgcgg acccagtgaa
     3121 gctcgtcggt ggggcggttc gggcgtacgg ccgtctcggc cggtgccgcg gccggcggcc
     3181 gcatcgcggg agcgcaggac gtcagggcga aggcgagagc ggcggaagcg acggcgatcg
     3241 ggaccttgct catcggaatt cccgagtcta gcagcgcccg gcctcgcgta ctctcccggg
     3301 ccatgggaaa gaggatcgag ccgtgccgga agagccccga agagcgcctc gacgacctcc
     3361 tcgcggggca tcgcgaggcg tctctgcgga acgagggggc gaagtacgcc gcgcgcgccc
     3421 tctcctcgtc cgactcgatg ccgaacggcg tgaagttctt cgcgtacgcc ctcctggcgg
//
LOCUS       DAOTEZ010000453         3445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_20810_length_3445_cov_4.917699, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000453 DAOTEZ010000000
VERSION     DAOTEZ010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20810_length_3445_cov_4.917699"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="P5164_17685"
     CDS             <1..439
                     /locus_tag="P5164_17685"
                     /inference="COORDINATES: protein motif:HMM:NF036831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRY45766.1"
                     /translation="ALGYPGAEEERALLVSGGADRALSTMRPVLTLTELKQAQAMVES
                     VTVAEKLLDYVMALVQRTRASRDLVLGVSPRGAQGLFRAVQAHALVAGRPFATPDDVK
                     KVAPAVLAHRVLASATGRYEGTGAGRRERETVQKILDEVAVPL"
     gene            462..1478
                     /locus_tag="P5164_17690"
     CDS             462..1478
                     /locus_tag="P5164_17690"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRY45767.1"
                     /translation="MKLSVLRLRLKVPWDETILVRVTNLGLGYVLTALVVAIGATNTG
                     NNGLYVLLSLLLGVLLVSGVVSRRNVDAVGVSLDGPAEVFAGEPARFRVRLENPTKRA
                     KTALLVKVSGKAAPLLFPQVEAGGEAERGVDLVFPRRGRQRVESLLVFSGYPIGLFRK
                     GRLHPVNEERIVFPSPADAAAPAPDHRDTSDEGREPRMRGRGAEILKLREAVPGDDPR
                     DIHWPQTARQGRPIIKERASELGGEVVVHLDTFRPASAGPSWDAAFEEAVRRATGLVL
                     TMLARGEKVGLLCGGTFAPPASGPYHRTTLLTALALVAPGAEAPLPLSLPAGTTLYHV
                     RARG"
     gene            1482..>3445
                     /locus_tag="P5164_17695"
     CDS             1482..>3445
                     /locus_tag="P5164_17695"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HRY45768.1"
                     /translation="MARKPRPDPALLPFRRERILAVGLVSALAPIPLAFTGALPPTAL
                     LVYLAALGVVLGFVKAGRVPCLADRWLNLAGVAYFALFWFGVRFGGRSLLRTALHILL
                     FTAVLKLASIKRERDFSVSLVLATFLLVASMATSTHSTILLFLGGYAVVAWPILSRWA
                     LWRDLAAAPDEWRRDERAREIPSRRAVAVSLGAAFALAIPMFVLFPRLKTSYIQGLPG
                     GDAGESGFSDTVDTNLYGRLRQSDKVFLRVVVEEGPLLDDPPFLRIRLLAHTRFSEGV
                     WRPPADRGRVVAMRDGNRLSLRPWGGPRPEATHRLSLEVAPLGIRFLPYPVTALSASA
                     SPELTRRWGVVPFVRDGDGNFRLTVEPPPLMRFEVLSAAEPLVDRTPPEPGDEATRPV
                     GSATIRDWGREVAAGVDPRADPWLFARRIEEHLATRYFYTLDSARWGPSAVEEFLTSR
                     RSGHCETFATAMALVLREHGIPTRLVTGFAGGERGPFGSYYLVRGREAHAWLEAWCGP
                     ERGWVSFDPTPASGRPQVTRLDLVRSVGQFFGNLEFLYGRWILGFAQADQASIATAVR
                     EAVAAIEEKAKAVARAVSSIAAGGGPARLLLGVLGAVALAALVLLIGRLARGVGGFGM
                     RGLPPASAAYRRLQRLLRRRGGALTPASAPS"
BASE COUNT          369 a         1294 c         1282 g          500 t
ORIGIN      
        1 cgcgctcggc tacccgggcg cggaggagga gcgggcgctc ctcgtctccg gcggcgccga
       61 ccgggcgctc tcgacgatgc gcccggtcct gacgctgacg gagctgaagc aggcgcaggc
      121 gatggtcgag tcggtcacgg tcgccgagaa gctcctcgac tacgtcatgg ccctcgtgca
      181 gcggacgcgc gcgagccgcg acctcgtcct cggggtctcg ccgcgcggcg cgcaggggct
      241 cttccgggcg gtgcaggcgc acgcgctcgt ggcggggcgg ccgttcgcga cgcccgacga
      301 cgtgaagaag gtggcccccg cggtcctcgc ccaccgggtc ctcgcgagcg cgacggggcg
      361 gtacgagggg acgggcgcgg ggcggcgcga gcgcgagacg gtccagaaga tcctggacga
      421 ggtcgccgtc ccgctctgag cgcggggcgg cccgaggcga cgtgaagctg tcggtcctcc
      481 ggctccggct gaaggtcccg tgggacgaga cgatcctcgt ccgcgtcacg aacctcggcc
      541 tcggatacgt cctgacggcc ctcgtcgtcg cgatcggcgc gacgaacacg gggaacaacg
      601 gcctctacgt cctcctctcg ctcctcctcg gcgtcctcct cgtctccggc gtcgtctcgc
      661 ggcggaacgt ggacgccgtg ggcgtctcgc tcgacggccc ggccgaggtc ttcgcgggcg
      721 agcccgcccg gttccgggtg cggctcgaga acccgacgaa gcgcgcgaag acggcgctcc
      781 tcgtgaaggt ctcggggaag gcggcgccgc tcctcttccc gcaggtcgag gccggaggcg
      841 aggccgagcg gggggtcgac ctcgtctttc cccgccgcgg gcggcagcgc gtcgagtcgc
      901 tcctcgtctt cagcggctat ccgatcggcc tcttccggaa ggggcggctc cacccggtga
      961 acgaggaacg gatcgtcttt ccgagccccg ccgacgcagc cgcgcccgcg ccggaccacc
     1021 gggacacgag cgacgaagga cgggagccgc ggatgcgcgg ccgcggggcg gagatcctga
     1081 agctccgcga ggcggtgccg ggggacgacc cccgggacat ccactggccc cagacggcgc
     1141 ggcaggggag gccgatcatc aaggagcggg cctcggagct gggcggagag gtcgtcgtcc
     1201 acctcgacac gttccgcccc gcgtccgccg ggccgtcctg ggacgcggcg ttcgaggagg
     1261 cggtccggcg cgcgaccggg ctcgtcctga cgatgctcgc gcggggggag aaggtcggcc
     1321 tcctctgcgg cgggacgttc gcgccgcccg cgagcggccc gtaccaccgg acgaccctcc
     1381 tgacggcgct cgcgctcgtc gcgccggggg ccgaggcccc tctcccgctc tcgctccctg
     1441 cgggaacgac gctctaccac gtgcgggcca ggggctgacc gatggcgagg aagccgcgcc
     1501 ccgacccggc gctcctcccg ttccggcggg agcggatact ggccgtcggc ctcgtctccg
     1561 cgctcgcccc gatcccgctg gcgttcacgg gggcgttgcc gcccaccgcg ctcctcgtct
     1621 acctcgccgc gctcggagtc gtcctcgggt tcgtgaaggc ggggcgggtc ccctgcctcg
     1681 cggaccgctg gctgaacctc gccggcgtcg cctacttcgc cctcttctgg ttcggcgtcc
     1741 gcttcggggg gcgctccctc cttcggacgg cgctccacat cctcctcttc acggccgtcc
     1801 tgaagctcgc gtcgatcaag cgggagcgcg acttctcggt ctcgctcgtc ctcgcgacgt
     1861 tccttctcgt cgcctcgatg gcgacctcga cgcactcgac gatcctcctc ttcctcggcg
     1921 gctacgcggt ggtggcgtgg ccgatcctgt cgcgatgggc gctctggcgg gacctcgcgg
     1981 ccgccccgga cgagtggcgg cgcgacgagc gggcccgcga gatcccctcc cggcgcgcgg
     2041 tcgcggtctc gctcggcgcc gccttcgcgc tcgcgatccc gatgttcgtc ctcttcccgc
     2101 ggctgaagac ctcgtacatc caggggctgc cgggaggcga cgccggggag agcggcttct
     2161 ccgacacggt cgacacgaac ctgtacggcc ggctccggca gagcgacaag gtcttcctcc
     2221 gcgtcgtcgt ggaggagggg ccgctcctcg acgacccgcc gttcctccgg atccggctcc
     2281 tcgcgcacac gcgcttctcg gagggggtct ggcggccgcc ggccgaccgg gggcgcgtcg
     2341 tggcgatgcg ggacgggaac cgcctttccc ttcgcccctg gggcggcccg cgaccggagg
     2401 cgacgcaccg tctctcgctc gaggtcgcgc cgctcggcat ccggttcctg ccgtatcccg
     2461 tcacggcgct ctcggcgtcc gcctcgcccg agctgacgcg ccggtggggc gtcgtgccgt
     2521 tcgtgaggga cggcgacggc aacttccgcc tcacggtgga gccgccgccg ctgatgcgct
     2581 tcgaggtcct ctcggcggcc gagcccctgg tcgacaggac gccgcccgag cccggagacg
     2641 aggcgacgcg tccggtcggg tcggcgacga tccgggactg gggccgcgag gtcgcggccg
     2701 gggtcgaccc gcgcgccgac ccgtggctct tcgcccggcg gatcgaggag cacctcgcga
     2761 cccgctactt ctacacgctc gactcggccc gctggggccc ctccgccgtc gaggagttcc
     2821 tgacgagccg gcgatcgggg cactgcgaga cgttcgcgac ggcgatggcg ctcgtcctgc
     2881 gcgagcacgg cattccgacg cggctcgtga ccggattcgc gggaggcgag cgggggccct
     2941 tcggctccta ctacctcgtc cgcggccggg aggcgcacgc ctggctcgag gcgtggtgcg
     3001 gcccggagcg gggctgggtc tcgttcgacc cgacgccggc ctcggggcgg ccgcaggtga
     3061 cgcgcctcga cctcgtccgg agcgtggggc agttcttcgg gaacctcgag ttcctctacg
     3121 gccgctggat cctcggcttc gcccaggccg accaggcgtc gatcgcgacg gccgtccgcg
     3181 aggcggtcgc ggcgatcgag gagaaggcga aggccgtcgc gcgggccgtc tcgtcgatcg
     3241 cggcgggcgg agggccggct cgcctcctcc tcggcgttct cggcgcggtg gcgctcgccg
     3301 cgctcgtcct cctcatcggg aggctcgccc gaggtgtcgg aggcttcggc atgcgggggc
     3361 tgccgcccgc ctcggcggcc tatcgacggc tccagcggct ccttcggcgg aggggcggcg
     3421 ccctgacgcc cgcctcggcc ccgtc
//
LOCUS       DAOTEZ010000454         3421 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_21020_length_3421_cov_4.987819, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000454 DAOTEZ010000000
VERSION     DAOTEZ010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21020_length_3421_cov_4.987819"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="P5164_17700"
     CDS             complement(<1..1080)
                     /locus_tag="P5164_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRY45769.1"
                     /translation="MKERTKLLLIVGFFLGAYFLPVESSRFQRAVPEGFVLLRDYARE
                     HVLLCLVPAFFIAGAIGQFVSQGAVMKYLGATAKRPVAYGVGSVSGAILAVCSCTVLP
                     LFSSIYKRGAGLGPATAFLYSGPAINVLAMILTARVLGLQIGLARAIGAVAFAVVIGL
                     IMAAIFREEEAERAKNGPDPFAAAGAAASGRTRAGWQDASFLGSMVAILVFANWGASN
                     RAVGFFAAVYEAHWYLAGAALAALVFMMVRWYDRDELRLWVLGSWEFAKQILPLLLGG
                     ILVAGWLMGRPGHDSGLIPDAWIAGAVGGNSLAANLFASVAGAFMYFATLTEVPILQG
                     LLGSGMGKGPALALLLAGPALSLPSM"
     gene            complement(1136..1387)
                     /locus_tag="P5164_17705"
     CDS             complement(1136..1387)
                     /locus_tag="P5164_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010879737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH895/ArsE family thioredoxin-like protein"
                     /protein_id="HRY45770.1"
                     /translation="MDTAPRKIEVLGPGCTRCKETFRVVSHVVETERLPFTVEKVEGM
                     ERMIELGLLATPGVAVDGKVIFSGRIPKAEELRAALAAL"
     gene            1615..2631
                     /locus_tag="P5164_17710"
     CDS             1615..2631
                     /locus_tag="P5164_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY45771.1"
                     /translation="MNRRTFLAALAAFPGARLLAAADAGPRMRVFGSLPRPERVRRVF
                     AAGPPAAVLAHVLSPERLIGWPMPLSPEARAFLAPAVRERPFLGRLSGRGSTIPLEKL
                     LALEPDLILDTGTVDATYLSAAENVHERTGIPYVLVPGRLADSPWQLSEAGRLLGVPE
                     RGATLAREAERLLTEARGALRAGARGPRVYVARGPDGLETVKEGSLNAESLALAGGRS
                     VADGPGGGVARVSFEQVAAWQPEVVLTQDAGFFASARSDDRWSRLPALRAGRLHLVPS
                     LPFGWLDGPPGVNRLVGLPWLASRLHGSPLGKEEVERVRAFHARFYGFSPERATLERM
                     LAGA"
     gene            2631..>3421
                     /locus_tag="P5164_17715"
     CDS             2631..>3421
                     /locus_tag="P5164_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRY45772.1"
                     /translation="MPRWPWLLLGLLLTLAVLAGLAAGPIALGPGELLRALLGGPSAE
                     GQATMVVWSLRLPRLGAALLVGAALSAAGTAYQAMFRNPLVSPDILGASAGAGMGAAL
                     AILFGLPTLGIQGAAFAGGLTAVGLVTLVAGRVRRHDPVLVLVLAGIAIGTLFSAGIS
                     LAKVLADPTVQLPSITFWLLGGLSASSPADVGRAAPLVAAGLLPLLLLRHRVDLLTLA
                     DEEAAALGEDVSRLRFTLIVSATLVTGASVAIAGIVGWVGLVVPHA"
BASE COUNT          428 a         1273 c         1260 g          460 t
ORIGIN      
        1 catgctcggc agggagaggg ccgggccggc gaggaggagc gccagcgcgg gccccttgcc
       61 catgcccgac ccgaggaggc cctgcaggat cggcacctcg gtgagcgtcg cgaagtacat
      121 gaaggcgccc gccacggagg cgaagaggtt cgcggcgagg gagttgccgc cgaccgcccc
      181 ggcgatccag gcgtccggga tcagccccga gtcgtggccg gggcggccca tgagccaccc
      241 ggcgacgagg atgccgccga ggaggagcgg gaggatctgc ttggcgaact cccacgagcc
      301 gaggacccag agacggagct cgtcccggtc gtaccagcgg accatcatga agacgagggc
      361 cgcgagggct gcgccggcga gataccagtg cgcctcgtag accgccgcga agaagccgac
      421 ggcgcggttg gaggcgcccc agttcgcgaa gacgaggatc gcgaccatcg agccgaggaa
      481 ggaggcatcc tgccaccccg cgcgcgtccg gccgctcgcg gcagcgcctg ccgcggcgaa
      541 cgggtcgggg ccgttcttcg cgcgctcggc ctcctcctca cgaaagatcg ccgccatgat
      601 caggccgatg acgacggcga aggcgacggc gccgatcgcg cgggcgaggc cgatctggag
      661 gcccagcacc cgcgccgtga ggatcatcgc gaggacgttg atcgcggggc ccgagtagag
      721 aaacgccgtg gccgggccga ggcccgcgcc gcgcttgtag atgctcgaga agaggggcag
      781 gaccgtgcag gagcagaccg cgaggatggc gccggagacc gagccgaccc cgtaggcgac
      841 cgggcgcttc gccgtcgcgc cgaggtactt catgacggcg ccctggctca cgaactggcc
      901 gatcgcgccc gcgatgaaga acgccgggac gagacagagg aggacgtgct cgcgcgcgta
      961 gtcgcggagc aggacgaacc cctcgggaac ggcccgctgg aagcgcgagc tctcgaccgg
     1021 caggaaatac gcgccgagga agaacccgac gatcaggagg agcttcgtcc tctccttcat
     1081 ggatggcctc cgggcgggcg ccgcgtccgc cgcagccacg ccccgtcgcg gggactcaga
     1141 gggcggcgag cgccgcgcgg agctcctcgg ccttcgggat gcggccggag aagatcacct
     1201 tcccgtcgac ggcgacgccg ggagtggcca ggaggccgag ctcgatcatc cgctccatcc
     1261 cctcgacctt ctcgacggtg aaggggaggc gctccgtctc gacgacgtgg gagacgacgc
     1321 ggaacgtctc cttgcagcgc gtgcagccgg ggccgaggac ttcgatcttt cggggggcgg
     1381 tgtccatgga acggtccttt cttcgggagg gaggggccgg cacccggccc tcgattcggc
     1441 tgatcacgaa tcatagttta ttgatggatg ggcgtccagg acgtcgccgg gaggaccggc
     1501 ggggacgccg cgggccggcg gctatcatcg ggtcgtgagc ctgcgcagaa cggcctcccg
     1561 gcgcgtcgac gtggcgccgc gaccccggtt cctctcgccg tcggccccgc cgcgatgaac
     1621 cgccgcacgt tcctcgccgc cctcgccgcg ttcccgggcg cacgcctcct cgccgcggcc
     1681 gacgccggac cccggatgcg cgtcttcggg agcctcccgc gccccgagcg ggtccgccgc
     1741 gtcttcgccg ccggccctcc ggcggccgtc ctcgcccacg tcctttcgcc ggagcgcctg
     1801 atcggctggc cgatgcctct ctcgccggag gcgcgcgcgt tcctcgcgcc cgcggtccgc
     1861 gaacgcccgt tcctcgggcg gctctcgggg cgcggcagca cgatcccgct cgagaagctc
     1921 ctcgcgctcg agcccgacct gatcctcgac accgggacgg tcgacgcgac ctacctctcc
     1981 gccgcggaga acgtccacga gcggaccggc atcccgtacg tcctcgtgcc ggggcgactc
     2041 gccgacagcc cgtggcagct ctcggaggcg gggcgcctcc tcggcgtgcc ggagcgcggc
     2101 gcaacgcttg cgcgcgaggc ggagcggctg ctcaccgagg cgcgcggggc gctccgcgcg
     2161 ggcgcgcgag ggccgcgcgt ctacgtcgcc cgcggcccgg acggcctcga gacggtgaag
     2221 gagggctcgc tcaacgccga gtcgctcgcc ctcgccgggg gacggagcgt cgcggacggc
     2281 ccggggggag gcgtcgcgcg cgtctcgttc gagcaggtgg cggcctggca gccggaggtc
     2341 gttctgacac aggacgccgg cttcttcgcg agcgcgcgga gcgacgaccg ctggtcgcgc
     2401 cttcccgccc ttcgtgcggg gcggctccac ctggtcccga gcctgccgtt cgggtggctc
     2461 gacggccctc cgggcgtcaa ccgcctcgtc ggcctcccgt ggctcgcctc gcgcctccac
     2521 ggctcgccgc tcgggaagga ggaggtcgag agggtccgcg cgttccacgc tcggttctac
     2581 ggcttctccc ccgagagggc gacgctcgag aggatgctgg cgggggcctg atgccgcggt
     2641 ggccctggct cctcctcgga ctcctcctca cgctggccgt cctcgccggg ctcgccgcgg
     2701 gcccgatcgc gctcgggccc ggcgagctcc tgcgcgcgct cctcggaggc ccctccgcgg
     2761 aggggcaggc gacgatggtc gtctggagcc ttcgcctccc gcggctcggc gcggccctcc
     2821 tcgtcggcgc ggccctctcg gccgccggga cggcctacca ggcgatgttc cgcaacccgc
     2881 tcgtctcgcc cgacatcctc ggagcctccg caggggcggg aatgggagcg gccctcgcga
     2941 ttctcttcgg tctgccgacg ctcgggatcc agggggcggc cttcgccggc ggcctcaccg
     3001 ccgtcggcct cgtcacgctc gtcgcggggc gcgtgcggcg gcacgacccg gtcctcgtcc
     3061 tcgtcctcgc cgggatcgcg atcgggaccc tcttctcggc cgggatctcg ctcgcgaaag
     3121 tcctcgcgga cccgacagtg cagctcccgt cgatcacctt ctggctcctc ggagggctca
     3181 gcgcctcctc tccggccgac gtggggcgcg cggcgccgct cgtcgcggcg gggctcctcc
     3241 cgctcctcct cctccgtcat cgggtcgacc tcctgacgct cgccgacgag gaggccgctg
     3301 cgctcgggga ggacgtctcc cgcctgcgct tcacgctcat cgtctccgcg acgctcgtga
     3361 cgggcgcctc cgtcgcgatc gcggggatcg tcggctgggt ggggctcgtc gtgccgcacg
     3421 c
//
LOCUS       DAOTEZ010000455         3404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_21191_length_3404_cov_5.552404, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000455 DAOTEZ010000000
VERSION     DAOTEZ010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21191_length_3404_cov_5.552404"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2581)
                     /locus_tag="P5164_17720"
     CDS             complement(<1..2581)
                     /locus_tag="P5164_17720"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HRY45773.1"
                     /translation="MRNDRPTSAAENAVSGLSRRALATPEPYVRLFSRRVQQEGALDL
                     TQGDYKNADFAPHPEVVRAAQRITRNTVHSYGPAIGRMDVRGEVADFFNRDGLLDYPQ
                     SPVRFLPDEVLFTPGTRSGLAMVLEVLGADGSGVVVPRPSWEYDWYVERAGKTLVELP
                     TTPPDFLPDPKELDRLLGKGGISSVILNNPHNPTGRVYPRELVEAIVRVAVKHRCYVL
                     YDSVYQRLDYVGWFVNPAFADPEWRDWVVSLSGLSKMDMFGASTGARACWMVLSDQIR
                     ANGVRAREILANLSAWLVATPSTLAQDWALAALQSPLAALRRPSPYMRERRDYMVKAA
                     DDLAPLGVTRTDFGGTFYAPLAFPGLVGEPFDRLRNGVTEKAVVKDSVDAFELLLSGG
                     VGGIPFVAFAGSDAARYGTWQRLSYGSKDVKELTVFIDRVRARIEAQGKLGSSAPAAG
                     AAATPLAAVWESVCTTSGYDALDGLDPKAFAKERKQFLKAPRHEELRLTGTKHPDSTA
                     HRAEQLARALNAAPAGPGRAKRALKHLEWNPLVDARLEYEETIADLLGPCSEVLLDFE
                     GRQISPEWLDLPEKVVLALLRHGVVPGEDRHLLFRVPNPFLEQDEEKIVRILASVARA
                     NALFHLASEKVGLTPSQNALPELTVPQVNSGAEIGAVVKIGALYRAAIASLFADDAHG
                     DAFLRARVPETRRAEMAAKLERVRLVPLCENVGALARLPEFLEAFYVALERGRSLPDV
                     PTAAVFRTRFDRDEAVVRVFVAMSDTAEQSGKVATDAAVTLALAGREEAEKRLAALAS
                     TAGEPAPRVTFLIGAGRAGFRGGFDPDHPGVIRQFSRADGVTMQGIRADDPAAAERLA
                     AAFR"
     gene            complement(2824..>3404)
                     /locus_tag="P5164_17725"
     CDS             complement(2824..>3404)
                     /locus_tag="P5164_17725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HRY45774.1"
                     /translation="GPQKAIGAPPGLALCLFSERALARRKARAAVPAYYADVLRWLPV
                     MEDPGKYFSTPCVNEIVALAEALRILHAEGLETRFARHARIARAFRAGLAALDLALFT
                     DAGARADTLSVVLLPEGVADGDFRREVTARGVVVAGGLGPIAGRAFRLGHMGNIGPAE
                     VATTLTAIEGALAALGRPVSPGSALAAAAAHL"
BASE COUNT          476 a         1140 c         1345 g          443 t
ORIGIN      
        1 cgcggaacgc ggcggcgagg cgttcggcgg cggccgggtc gtccgcgcgg atcccctgca
       61 tcgtgacgcc gtcggcccgt gaaaactgcc ggatcacgcc cgggtggtcg gggtcgaagc
      121 cgccccggaa gccggcccgc ccggcgccga tgaggaacgt gacgcgcggc gccggctcgc
      181 cggccgtgct cgcgagcgcg gcgaggcgct tctcggcctc ctcgcgtccg gcgagggcaa
      241 gcgtgacggc ggcgtcggtc gccaccttgc cgctctgctc ggcggtgtcg gacatggcga
      301 cgaagacgcg gacgaccgcc tcgtcgcggt cgaaccgagt ccggaagacc gcggccgtcg
      361 ggacgtcggg gagagagcgc ccccgctcga gggcgacgta gaacgcctcg aggaattcgg
      421 ggaggcgcgc gagggcgccg acgttctcgc agagggggac gaggcggaca cgctcgagct
      481 tcgcggccat ctccgcacgg cgcgtctcgg ggacccgcgc gcggaggaag gcgtcgccgt
      541 gcgcgtcgtc ggcgaagagg gaggcgatcg ccgcgcggta gagcgcaccg atcttcacga
      601 ccgcgccgat ctcggcgccg gagttcacct gcgggacggt cagctccggg agcgcgttct
      661 gcgacggcgt cagcccgacc ttctcggagg cgaggtggaa gagggcgttc gcgcgcgcga
      721 ccgaggcgag gattcggacg atcttctcct cgtcctgctc gaggaacggg ttcgggaccc
      781 ggaagaggag gtggcggtcc tcgcccggga cgacgccgtg gcggaggagg gcgaggacga
      841 ccttctccgg gaggtcgagc cactcggggc tgatctggcg tccctcgaag tcgagcagga
      901 cttcgctgca cggcccgagg aggtcggcga tcgtctcctc gtactcgagg cgcgcgtcga
      961 cgagcgggtt ccactcgagg tgcttgagcg cccgcttcgc gcggccgggg ccggcgggcg
     1021 cggcgttgag ggcgcgggcg agctgctcgg cgcggtgcgc cgtcgagtcg gggtgcttcg
     1081 tcccggtgag ccggagctcc tcgtggcgcg gcgccttcag gaactgcttc ctctccttcg
     1141 cgaacgcctt cgggtcgagg ccgtcgagcg cgtcgtagcc cgaagtcgtg cagacgctct
     1201 cccagacggc cgcgagcggc gtcgccgcgg ccccggcggc cggggcgctc gacccgagct
     1261 tcccctgcgc ctcgatccgc gctctcacgc ggtcgatgaa gacggtcagc tccttgacgt
     1321 ccttcgagcc gtaggagagc cgctgccacg ttccgtagcg cgcggcgtcg gagccggcga
     1381 aggcgacgaa cgggatcccg cccacgccgc ccgacaggag gagctcgaag gcgtcgaccg
     1441 agtccttcac cacggccttc tccgtcacgc cgttcctcag gcggtcgaac ggctcgccga
     1501 cgaggccggg gaaggcgagc ggggcgtaga acgtcccgcc gaagtcggtc ctcgtgacgc
     1561 cgagcggcgc gaggtcgtcg gcggccttca ccatgtagtc gcgccgctcg cgcatgtagg
     1621 gcgaggggcg ccggagggcc gcgagcgggc tctggagcgc ggcgagcgcc cagtcctgcg
     1681 cgagcgtcga gggggtggcg acgagccagg cggagaggtt cgcgaggatc tcgcgggccc
     1741 tcacgccgtt cgcgcggatc tggtcggaga ggaccatcca gcaggcgcgc gcgccggtcg
     1801 aggccccgaa catgtccatc ttcgagaggc cggagagcga gacgacccag tcgcgccact
     1861 cggggtcggc gaacgcgggg ttcacgaacc agccgacgta gtcgagccgc tggtagacgg
     1921 agtcgtagag gacgtagcag cggtgcttca ccgcgacccg gacgatcgcc tcgacgagct
     1981 ccctcgggta gacgcggccc gtcgggttgt gggggttgtt caggatgacg gaggagatgc
     2041 cgcccttgcc gaggagccgg tcgagctcct tcgggtcggg gaggaagtcg gggggcgtcg
     2101 tcgggagctc gacgagcgtc ttgccggccc gctcgacgta ccagtcgtat tcccagctcg
     2161 gccgcgggac gacgacgccc gagccgtcgg ccccgaggac ctcgaggacc atcgcgagcc
     2221 ccgagcgggt ccccggcgtg aagaggacct cgtcggggag gaagcggacg ggcgactgcg
     2281 ggtagtcgag gaggccgtcg cggttgaaga agtcggcgac ttcgccgcgg acgtccatcc
     2341 gcccgatggc cggcccgtag ctgtggacgg tgttccgggt gatccgctgg gccgcccgga
     2401 ccacctccgg gtgcggcgcg aagtcggcgt tcttgtagtc gccctgggtc aggtcgaggg
     2461 ccccttcctg ctggacgcgc cgggagaaga ggcggacgta aggctcgggc gtcgcgaggg
     2521 cgcgcctcga gaggccggag acggcattct ccgccgcgga cgtcggacgg tcgttcctca
     2581 tggcgtgccc tccctgtgcc tcgcacggag ccgggatcgc tccccggccg gatcgtctgc
     2641 ctcgcttcgg tgcggcggga gtctgcctga aaccccgtcc agtcgcagca aacctcgcag
     2701 agtgtagaag gtagacctga ccccggggcc agatctaccg tctacactct gcggtcgtac
     2761 gaggggacgc gggaacgccg cgacggctcc gggagggagg ccgacctccg gagcggccga
     2821 acgtcagagg tgtgcggcgg cggcggcgag ggccgagccg ggagagacgg ggcggccgag
     2881 ggcggcgagg gccccctcga tcgcggtgag cgtcgtcgcg acctcggccg ggccgatgtt
     2941 ccccatgtgc ccgaggcgga aagccctccc ggcgatcggg ccgaggccgc ccgcaacgac
     3001 gacgccccgc gcggtcacct cgcgtcggaa atcgccgtcg gcaacgccct cgggaaggag
     3061 gacgacggag agcgtgtcag cgcgggcgcc cgcgtcggtg aagagcgcga ggtcgagcgc
     3121 cgcgaggccg gcacggaagg cgcgcgcgat ccgcgcgtgg cgcgcgaaac gggtctcgag
     3181 cccctcggcg tgaaggatcc ggagcgcctc ggcgagcgcg acgatctcgt tcacgcaggg
     3241 cgtcgagaag tactttccgg ggtcctccat gacggggagc cagcggagta cgtcggcgta
     3301 gtaggccgga accgcggcgc gggccttccg ccgggccagc gctcgctccg agaagaggca
     3361 gagcgcgagg ccgggagggg caccgatcgc cttctgcggc ccgg
//
LOCUS       DAOTEZ010000456         3399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_21243_length_3399_cov_4.926734, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000456 DAOTEZ010000000
VERSION     DAOTEZ010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21243_length_3399_cov_4.926734"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="P5164_17730"
     CDS             <1..532
                     /locus_tag="P5164_17730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45775.1"
                     /translation="KDILKNYFGKTKTFGLLEVRGSYPIMVTSNTYNVAGAIAGTYGQ
                     YSPGQPDRNALGFDDSIRGNLYINGLTNDPVLRTNAVVMNPSGKRLEAGVQLVDAMGQ
                     VLGTRIYEVEPYSLHQINDVFGSEFRSFNPPAGGPWRLNLFVNLDNGALILGYATVTD
                     TRTGDPYLIPAEAMGP"
     gene            529..1185
                     /locus_tag="P5164_17735"
     CDS             529..1185
                     /locus_tag="P5164_17735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRY45776.1"
                     /translation="MTRALLVDLGKVLVGFDHQVTCEKMSKATGVPAGRLRGVLFGEL
                     EGDFDRGRLTPGAFFRACEARLGILPVPDETWIAAWRDIFTPLPEAIAALGRVRPGIR
                     RVLVSNTNVLHWEGVLSVFRPDALLDDVVLSFRVGATKPEPAFWDAALAAAGCGPAAC
                     VYADDRPELVAAASALGIPGFVVDSPASLARGLARFDLLAPSSTASGNSPAATDVPRR
                     "
     gene            1187..1648
                     /locus_tag="P5164_17740"
     CDS             1187..1648
                     /locus_tag="P5164_17740"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HRY45777.1"
                     /translation="MTILTRRAPRLLAVALLALLPTAACSRSEAPAETSISSGDGIAF
                     SAGGFEEALTRARTEKRLLLVDVYTDWCGWCKKLDREVFADGRVGSAAKDLVAVKVNA
                     EKGGEEIARRYRVRGYPTILFLDGEGKVVERIDGYVDAGEMVKIVSALPKG"
     gene            complement(1994..>3399)
                     /locus_tag="P5164_17745"
     CDS             complement(1994..>3399)
                     /locus_tag="P5164_17745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY45778.1"
                     /translation="RRGPRHLLGRGGVVAPPGGRPRVSVAPLAFVSPLPPVRSGIADY
                     SADLLAALREEVELETYAPSEAATAFRRGHGAVLLQVGNDPLHLPSVEALRENAGRVP
                     AILVLHDFSLHHLFAAGYLDVARVEDYARELERSHGAKGRALGDRARAGERVPVWDLA
                     PWDWPMSLGVVGDAAAVIVHSSLVRGALLRRGVRAPVFEVPHLVAPAPEHDRADARAA
                     LDLPPDRVVAATLGIVTPAKRIGRLLEGLAILPPAERPFLVVGGAVADDDPLRDLVRE
                     HGLAGDVRFTGYLAEEDFWRLARAADVSVNLRFPTVGETSGAVCRLAGTGLPVVVSDV
                     GWFRELPDSFAAKVPIGAGEVEAIAAVLGELVRDAGVRRARAAAAAAWGVERSPARIA
                     AAYARVVRAVLEGRAPALALPSRLAGALSAVGIGRPGVHGSVERSPDGGLVATAASRA
                     IGVLPRPLVPFLRTSDA"
BASE COUNT          491 a         1274 c         1190 g          444 t
ORIGIN      
        1 gaaggacatc ctcaagaact acttcggcaa gacgaagacg ttcgggctcc tcgaggtgcg
       61 gggcagctac ccgatcatgg tgacgagcaa cacctacaac gtcgcgggag cgatcgccgg
      121 gacttacggc cagtactctc ccggccagcc cgaccggaac gcgctcgggt tcgacgattc
      181 gatccgcggg aacctctaca tcaacggcct cacgaacgac cccgtgctcc gcacgaacgc
      241 cgtcgtcatg aacccgagcg gaaagaggct cgaggccggc gtgcagctcg tcgacgcgat
      301 ggggcaagtc ctgggaacgc gtatctacga ggtggagccc tattccctcc accagatcaa
      361 cgacgtcttc ggttccgagt tccgctcgtt caatcctccc gcgggcggcc cctggcgcct
      421 caacctcttc gtcaacctcg acaacggcgc gctcatcctc ggctacgcga ccgtcaccga
      481 cacccggacg ggcgacccgt acctgatccc cgccgaagcc atggggccgt gacgcgggcc
      541 cttctcgtcg acctcgggaa ggtcctcgtc gggttcgacc accaggtcac gtgcgagaag
      601 atgtcgaagg cgaccggcgt gcccgccggt cgccttcgcg gcgttctctt cggggagctc
      661 gagggagatt tcgaccgcgg acgcctgacg cccggcgcgt tcttccgggc ctgcgaggcg
      721 aggctcggga tcctccccgt cccggacgag acctggatcg cggcctggcg tgacatcttc
      781 acaccgctgc ccgaggcgat cgcggcgctc gggcgcgtcc gaccggggat ccgtcgcgtc
      841 ctggtctcaa acacgaacgt tctccactgg gagggcgtcc tctcggtctt tcgccccgac
      901 gcgctgctcg acgacgtcgt cctctccttc cgcgtcggcg ccaccaagcc ggagccggcc
      961 ttctgggacg ccgcactcgc ggccgcgggc tgcgggcccg ccgcgtgcgt ctacgccgac
     1021 gaccggcccg agctcgtcgc ggccgcatcc gccctcggga tacccggctt cgtcgtggac
     1081 agccccgcgt ccctcgcccg gggtctcgcc cggttcgacc ttctcgcccc atcctccacc
     1141 gcttccggga attccccggc ggcgaccgac gttccgagac gctgatatga cgatccttac
     1201 ccgtcgtgcg ccccgcctcc tcgccgtcgc cctgctcgcc cttctcccga cggccgcgtg
     1261 cagccggtcc gaggctcccg ccgagacgtc cataagctcc ggggacggga tcgcgttctc
     1321 cgccggcgga ttcgaagagg cgctcacccg cgccaggacg gagaagcgcc tccttctcgt
     1381 cgacgtctac acggactggt gcggctggtg caagaagctc gaccgggagg tcttcgcgga
     1441 cggccgcgtc ggctctgccg cgaaggacct cgtcgccgtg aaggtgaacg ccgagaaggg
     1501 gggcgaggag atcgcccgcc gctaccgcgt ccgcggctac ccgacgatcc tcttcctcga
     1561 cggcgagggg aaggtcgtcg agcggatcga cggctacgtc gacgcgggcg agatggtgaa
     1621 gatcgtctcg gccctcccga agggctgacg attcctctcg accccccgtc gggctctctc
     1681 gcccgcggac tcgacgcgtt ccaggaggga cgctggttcg aggctcacga ggagtgggag
     1741 acggcctgga gggccgcggt cgatccggac cggcggcgcc ttcaggggct gatccaggcc
     1801 gcggccgccg cccttcacct cgaggcggga cggcgcgccc cggcggagag gctcctcgcg
     1861 ctcgcccttt cgaagctcga ggacgccccg gacgacgtcg gaggcctccc tgcgcgacgg
     1921 atccgcgacg gggcgtttcg tatcgcctcc gcgctcgccg caggcgagga ccctctcccc
     1981 gccgtcggcg acctcaggcg tctgaggtcc tgaggaacgg aacgagcggc cgcggaagga
     2041 ctccgatcgc ccgcgaggcc gccgtggcga cgagccctcc gtccgggctc cgctccaccg
     2101 acccgtggac gccgggacgg ccgattccga ccgcggagag ggctcccgcg agccgggacg
     2161 ggagagcgag ggcgggcgcc cggccttcga ggaccgcccg gacgacccgg gcgtaggccg
     2221 ccgcgatccg ggccgggctc cgctcgacgc cccaggccgc cgccgccgcc gcacgggcac
     2281 ggcgcacccc ggcgtcccgg acgagctctc cgagcacggc ggcgatcgcc tccacctcgc
     2341 cggctccgat cggcaccttc gccgcgaagg aatcaggcag ctcgcggaac caaccgacgt
     2401 cgctgacgac gaccgggagg cccgtcccgg cgagccggca gaccgcgccg gaggtctctc
     2461 cgacggtcgg gaagcggagg ttcaccgaga cgtccgctgc gcgggcgagc cgccagaagt
     2521 cctcttccgc gaggtagccg gtgaaacgga cgtcgccggc gagcccgtgc tcgcggacga
     2581 gatcgcgcag agggtcgtcg tccgcgacgg cgcctccgac gacgaggaac ggccgttccg
     2641 ccgggggcag gatcgcgagc ccctcgagga gccgcccgat ccgcttggcg ggcgtgacga
     2701 tgccgagcgt cgccgccacg acgcgatcgg gcgggaggtc cagcgcggcc cgcgcatcgg
     2761 cgcggtcgtg ctcgggggcc ggtgcgacga ggtgcgggac ctcgaagacc ggcgcgcgga
     2821 cgccccgtcg caggagcgcg ccgcgcacga ggctcgagtg gacgatgacg gcggcggcgt
     2881 ccccgacgac gccgagcgac atcggccagt cccacggcgc caggtcccag acggggaccc
     2941 gctcccccgc acgcgccctg tcgccgagcg cgcggccctt cgccccgtgc gaccgctcga
     3001 gctcgcgcgc gtagtcctcc acgcgcgcca cgtcgaggta gcccgcggcg aagagatggt
     3061 ggagcgagaa gtcgtggagg accaggatcg cgggcaccct ccccgcgttc tctcggagcg
     3121 cctcgaccga cggaaggtgg agcgggtcgt tcccgacctg caggaggacg gcgccgtgcc
     3181 cgcgccggaa ggccgtggca gcctccgacg gagcgtaggt ctcgagctcc acctcctctc
     3241 gaagggccgc gagaaggtcg gccgaatagt ccgcgatgcc cgagcggacc ggcgggagcg
     3301 ggctgacgaa ggcgagcggc gcgacgctca cccgaggccg cccgcccgga ggagcgacga
     3361 caccaccgcg tcccaggaga tgccgcggac cgcgtcgta
//
LOCUS       DAOTEZ010000457         3391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_21297_length_3391_cov_10.019784, whole genome shotgun
            sequence.
ACCESSION   DAOTEZ010000457 DAOTEZ010000000
VERSION     DAOTEZ010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21297_length_3391_cov_10.019784"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="P5164_17750"
     CDS             <1..454
                     /locus_tag="P5164_17750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45779.1"
                     /translation="RRPRRVNPARGGRPRRGRREALVLLDSFWVLVVLFGLGWCLALV
                     GAAVVDALIYRGSLNRTKLYELSSPSRPGGAVFSRVERRGGGTGFLRVEIERWLRGFP
                     VRDKEKAAGASGDQPKGKAEIRRLDASVSTVTPGRAPVKVAAGREGNP"
     gene            451..1446
                     /locus_tag="P5164_17755"
     CDS             451..1446
                     /locus_tag="P5164_17755"
                     /inference="COORDINATES: protein motif:HMM:NF021128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HRY45780.1"
                     /translation="MNAVETRALEPGSPEWHAERRKGIGGSDWKDVLNLEPYGCRRRL
                     YFEKTATPPDFPDRDNGRLRRGHALEPIVVEEYVRITGNRVTGPTPRPTQEWLPGWWV
                     GNPDRIIAAPDARRGPGVLECKTANEWVYWQAVSEGASEAWVAQLHHYLVLGSYRWGE
                     IAVLEVDSFRLFREELERDPELVDLMLRRGEEFWAAVQTRQTPGALDPSDKRCRTCPW
                     RITCHGAELYDPEFAPEPTDWEDSDDPEIGRLLEDYADLREKAKEADKALEEQRVRLH
                     NRLGWRPRKIKAGPWKVELRRTVRRTLDADRIKADHPELVKTHRNESVSFSLLVK"
     gene            1458..2492
                     /locus_tag="P5164_17760"
     CDS             1458..2492
                     /locus_tag="P5164_17760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45781.1"
                     /translation="MSAPQPATQEPGAAIVRQPEGVLTPRGIVPRNFNELWSFARVVA
                     GSRLAPRGLESAEAVFVAVETGLELGFSPIQALSSIAVVNGRAALLGMAAKALVLGSG
                     VCERWTEEALHVTVEEEEHLPDGVFGFRVRSKRVGLEDWTESTFTLADARRAGLWPGK
                     ADSNWAKYPARMLKMRAVGFVARDLYPDVLKGLHLAEEMEGTEPEPAAPPSNVARLKD
                     RLAEPIEDPRFEPPTCVETVTPDPGSIDIETISGNAVDAETVEPGDGLTDEELAQLLP
                     KKTGNWHRTCQKLLADGIVALGSARAYYKAALFVFGSHGATAADEITPADRKDVQAEI
                     EAAFAQAVKP"
     gene            2489..3217
                     /locus_tag="P5164_17765"
     CDS             2489..3217
                     /locus_tag="P5164_17765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45782.1"
                     /translation="MSAIVELTEAQRSVSTQALTWPEQAAAIRVVDEQTYTMAGTMLR
                     GVKGLMKEADNVFDPAVKKAHEAHKAIVAAKNAVVAPLREAETILKRGLADYTAEQER
                     RRLEEERRLQLEADRKAAEERKRLEDERKAELAKAALEGAAKLNAVAAQPAPPPPLAP
                     VVRLAPQTPKVSGVSTRTIWRAEVVDFAALVRYVADHAAEDPNVLSFLTVNLTPLTGI
                     ARGSEGKAAIPGVTFRAETVVASR"
BASE COUNT          572 a         1219 c         1150 g          450 t
ORIGIN      
        1 gcgacggccc cgccgtgtca atccagcgag gggaggtcgg ccgcgacgcg gccggcggga
       61 ggccctcgtg ttgctggatt cgttctgggt gctggtcgtt ctgttcggtc tggggtggtg
      121 cctggcgctc gttggcgccg cggtcgttga cgccctgatc taccggggct ccctcaaccg
      181 gacgaagctc tacgagctct ccagcccgag ccgcccggga ggggcagttt tctcccgggt
      241 cgagcggcgc ggcgggggaa ctggattcct ccgcgtcgag atcgagcgct ggcttcgggg
      301 attccccgtc cgagacaaag aaaaggccgc cggggcgtcc ggcgaccaac ccaaaggaaa
      361 ggccgagatc cgtcgactcg acgcttccgt ctcgaccgta accccgggcc gcgcccctgt
      421 caaggtcgcg gccggaaggg agggcaaccc gtgaacgccg tcgaaacccg agccctcgaa
      481 cccgggtcgc ccgagtggca cgccgaacgc cggaagggga tcgggggctc ggactggaag
      541 gacgtcctca acctcgagcc ctacggctgc cggcgccgcc tgtacttcga gaagacggcg
      601 accccgccgg acttcccgga tcgtgacaac ggccggctcc gccggggcca cgccctcgag
      661 ccgatcgtcg tcgaggagta cgtccggatc accgggaacc gtgtcaccgg cccgaccccg
      721 aggccgaccc aggagtggct ccccggatgg tgggtcggga acccggaccg gatcatcgcc
      781 gctcccgatg cgcggcgagg cccgggcgtc ctcgagtgca agaccgcaaa cgagtgggtc
      841 tactggcagg ccgtgtcgga gggggcctcg gaggcctggg tcgcccagct ccaccactac
      901 ctcgtcctcg gctcctaccg ctggggagag atcgccgtcc tcgaggtcga ctccttccgg
      961 ctcttccgcg aggagctcga gcgcgatccg gagctcgtcg acttgatgct ccggcgcggc
     1021 gaggaattct gggcagccgt ccagacccgc cagaccccgg gagccctcga cccctccgac
     1081 aagcgctgcc ggacgtgccc gtggcggatc acctgtcacg gcgccgagct ctacgacccc
     1141 gaattcgccc cagagccgac cgactgggag gactcggacg acccggagat cggacgactc
     1201 ctcgaggact acgccgacct ccgcgagaag gcgaaggagg ccgacaaggc cctcgaggag
     1261 cagagggtcc ggctccacaa ccggctcggc tggcgccccc ggaagatcaa ggccggcccg
     1321 tggaaggtcg agctccgccg gaccgtccgc cggaccctcg acgccgaccg gatcaaggcc
     1381 gaccaccccg agctcgtgaa gacccaccgg aacgagagcg tgagcttctc gctcctcgtc
     1441 aagtgaaggg atagaccatg agcgcaccgc aaccagcaac ccaggaaccg ggcgcggcga
     1501 tcgtccgcca gcccgagggc gtcctcacgc cccgggggat cgtcccccga aacttcaacg
     1561 agctctggag cttcgcccgc gtggtcgccg gctcccgact ggccccgcgg gggctcgaga
     1621 gcgccgaggc cgtcttcgtc gccgtcgaga ccgggctcga gctcggcttc tcgccgatcc
     1681 aggccctctc gtcgatcgcc gtcgtcaacg gtcgggcggc cctcctcggc atggccgcga
     1741 aggccctcgt cctcggctcc ggcgtctgcg agcgctggac cgaggaagcc ctccacgtca
     1801 cggtcgagga ggaggagcac ctccccgacg gcgtcttcgg cttccgcgtc cgctcgaagc
     1861 gcgtcggcct cgaggactgg accgaatcca ccttcaccct cgccgacgcc cggagggccg
     1921 gcctgtggcc ggggaaggcc gactcgaact gggcgaagta cccggcccgg atgctcaaga
     1981 tgagggccgt cggcttcgtc gcccgcgacc tctaccccga cgtcctgaag ggcctccacc
     2041 tcgccgaaga gatggagggg acagagccgg agcccgcggc cccaccctcg aacgtcgccc
     2101 gcttgaagga cagactcgcc gagccgatcg aggacccgcg cttcgagcct ccgacctgcg
     2161 tcgagaccgt gacgccggac cccggctcga tcgacatcga gaccatctcg gggaacgccg
     2221 ttgacgccga gacggtggag cctggggacg gcctgaccga cgaggagctc gcccagctcc
     2281 tcccgaagaa gaccgggaac tggcaccgga cgtgccagaa gctcctcgcc gacgggatcg
     2341 tcgccctcgg ctccgcccgc gcctactaca aggccgccct cttcgtcttc ggctcgcacg
     2401 gcgccaccgc tgccgacgag atcacgccgg ccgatcggaa ggacgtccag gccgagattg
     2461 aagcagcgtt cgcccaggcg gtgaagccat gagcgcgatc gtcgagctca ccgaagccca
     2521 gcgctccgtc tcgacccagg ccctcacctg gcccgaacag gcggcggcga tccgcgtcgt
     2581 cgacgagcaa acctacacga tggccgggac gatgctccgg ggcgtcaagg ggctgatgaa
     2641 ggaagccgac aacgtcttcg acccggccgt caagaaagcc cacgaggccc acaaggcgat
     2701 cgtcgccgcg aagaacgcgg tcgtcgcccc gctccgtgag gccgagacca tcctcaagcg
     2761 ggggctcgca gactacacgg ccgagcagga acgccgccgg ctcgaggagg agcgccggct
     2821 ccagctcgag gccgaccgga aggccgccga ggagcggaag cggctcgagg acgagcgcaa
     2881 ggccgaactg gcgaaggccg cgctcgaggg ggccgcgaag ctcaacgcgg tagcagcaca
     2941 gccggccccg cccccgccgc tcgcgccggt cgtccggctc gccccgcaga ctccgaaggt
     3001 ctccggcgtc tcgacgagga cgatctggag ggccgaggtc gtcgacttcg ctgcgctcgt
     3061 ccgctacgtc gcagaccacg ccgccgagga cccgaatgtc ctctcgttcc tgaccgtcaa
     3121 cctcacgcca ttgaccggga tcgcccgagg gtcggaaggg aaggccgcga tccctggcgt
     3181 caccttccgc gccgaaaccg tggtggcgag ccgatgaatt gaggaaggag acgtcatgaa
     3241 ctatcgcgat gtctttacgc aaggggattt gatgaaggcg atcactgacg gattgaaatg
     3301 gattcgcctg cgtggtactg gagtctttga gatcgactcc gcccaggtga gggcctacgg
     3361 ctccgcccag gtgaaggcct gcggctccgc c
//
LOCUS       DAOTEZ010000458         3388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_21323_length_3388_cov_6.920492, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000458 DAOTEZ010000000
VERSION     DAOTEZ010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21323_length_3388_cov_6.920492"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..780)
                     /gene="purQ"
                     /locus_tag="P5164_17770"
     CDS             complement(61..780)
                     /gene="purQ"
                     /locus_tag="P5164_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HRY45783.1"
                     /translation="MSSGARPRVSVVLFPGSNCDHDAVHAVETAIGADARLVWHDGTD
                     LGRPDLVVLPGGFSYGDYLRCGALARFSPVMDSVRDFAASGGPVLGICNGFQVLCEAG
                     LLPGALLANSGQRFLCQDVHVRVETIRTPFTSDLTKGDVLRLPIAHGDGNWRAEEATW
                     DDVVAHDQVVFRYCDAAGERVASANPNGSHDDVAGVCNRARNVVGLMPHPERASEEIL
                     GNADGARLFRSFASGLLGRSA"
     gene            complement(777..1019)
                     /gene="purS"
                     /locus_tag="P5164_17775"
     CDS             complement(777..1019)
                     /gene="purS"
                     /locus_tag="P5164_17775"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HRY45784.1"
                     /translation="MKVQVTVVPKSGVLDPQGKAIAGALTRLGFPGVGDVRAGKVFRV
                     EVEAKDAAEAKELAAQMAVKLLCNPVIEEFEAEVLG"
     gene            complement(1096..>1350)
                     /locus_tag="P5164_17780"
     CDS             complement(1096..>1350)
                     /locus_tag="P5164_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45785.1"
                     /translation="GAYPAFLKKRILSDVGHLSNEAAADGLERLVGERTRTLVGLHLS
                     QNNNTPTLAERALRRGLERLGAQVALELAGPSAPTGWFRA"
     assembly_gap    1351..1450
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1473..1934)
                     /gene="dut"
                     /locus_tag="P5164_17785"
     CDS             complement(1473..1934)
                     /gene="dut"
                     /locus_tag="P5164_17785"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRY45786.1"
                     /translation="MTPGASVTVRFRLRAECADLGPPLYASDLAAGADLRAALEEPLT
                     LAPGARAVVPTGLTLEIPPGFEAQVRARSGLALKKGLALANGVGTIDADYRGEVGVIV
                     VNLGAAPVTIERGERIAQLVVAPVVRAVFERAPELNGTARGAGGFGSTGTA"
     gene            complement(1931..3214)
                     /locus_tag="P5164_17790"
     CDS             complement(1931..3214)
                     /locus_tag="P5164_17790"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRY45787.1"
                     /translation="METRTLGDGLTVWLDPMRDVRSVALGVYVAGGSADEEASDLGST
                     HFLEHLLFRRARRRTGADIARTTDRLGGDCDAYTTKEWMAVHARTPSERLPDALSLLL
                     DLTEAPAFTADDVETERKVILEEMAEASDVPEDRLHETFVRSYWPDHPLGAPILGTEE
                     TVGALTRARLVRRFREVFRPERTLLVAVGAFEPEAFLKLLSTERRRRRHAAAPLGPVR
                     QAGPSPRGNRCAFHIPRPDLNQTHLLVGTPAPAYANRLVPAAWLLSVVLGGGVSSRLW
                     RRVRERHGLAYQVGAGLSLHRDGGMALIEAATAPKKLPKLVRATGAVLRELRQTGVTR
                     AELRRAKDQVRAEVAMSLESTSSRRESAVRGWFYRGRPWEADETIATMEAVTSADVDD
                     AIAALFGEPDASGFGVTGPTLVGATVDDLAGELAA"
BASE COUNT          418 a         1188 c         1291 g          391 t
ORIGIN      
        1 cccggacgag gggccccggg gctcgccgtc gcggagtccg cgcgggccgc gcccgccccg
       61 tcaggccgag cggccgagca gccccgaggc gaaggagcgg aagagcctcg ccccgtcggc
      121 gttgccgagg atctcctcgg aggcgcgctc ggggtgcggc atcaggccga cgacgttccg
      181 cgcgcggttg cagacgccgg cgacgtcgtc gtgcgagccg ttggggttcg cggaggcgac
      241 ccgctcgccg gccgcgtcgc agtagcggaa gacgacctgg tcgtgggcga cgacgtcgtc
      301 ccacgtcgcc tcctcggcgc gccagttccc gtcgccgtgc gcgatcggca gccggaggac
      361 gtcccccttc gtcaggtcgg aggtgaacgg ggtccggatc gtctcgacgc ggacgtggac
      421 gtcctggcag aggaagcgct ggccggagtt cgcgaggagc gcgcccggca ggagccccgc
      481 ctcgcagagg acctggaaac cgttgcagat gccgaggacc gggccgccgc tcgccgcgaa
      541 gtcccgaacc gagtccatca cgggggagaa gcgggcgagc gccccgcagc ggaggtagtc
      601 gccgtaggag aagccgcccg ggaggacgac gaggtcgggg cggccgaggt cggtgccgtc
      661 gtgccagacg aggcgcgcgt cggcgccgat cgccgtctcg acggcgtgga cggcgtcgtg
      721 gtcgcagttc gagccgggga agaggacgac cgagacgcgc ggccgcgcgc cggagctcaa
      781 ccgagcacct cggcctcgaa ctcctcgatg accgggttgc agaggagctt gacggccatc
      841 tgcgccgcaa gctccttcgc ctcggcggcg tccttcgcct cgacctcgac ccggaagacc
      901 ttccccgccc gcacgtcccc gacgcccggg aagccgaggc gcgtgagcgc gccggcgatc
      961 gccttcccct gcggatcgag gacgccggac ttcgggacga ccgtgacctg gaccttcatg
     1021 cgttcctcct ggggcgggcg gccggtcgcg gccctccccg atcgttctgc gcgccgcacc
     1081 gcgccggtcc gagcctcacg cccggaacca cccggtcggg gcggacggcc ccgcgagctc
     1141 gagggcgacc tgcgccccga gccgctcgag accccggcgg agcgcgcgct ccgcgagggt
     1201 cggcgtgttg ttgttctggg agaggtggag gccgacgagc gtccgcgtcc gctccccgac
     1261 gaggcgctcg agcccgtcgg cggccgcttc gttcgagagg tgccccacgt cggagaggat
     1321 cctcttcttc aggaaggccg ggtaggcccc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn gagccgagga aggcgatctc gatcacgccg tccccgtcga gccgaagccc
     1501 cccgcgccgc gggcggtgcc gttcagctcg ggcgccctct cgaagacggc gcggacgacc
     1561 ggagcgacga cgagctgggc gatccgctct ccgcgctcga tggtgaccgg cgcggcgccg
     1621 aggttcacga cgatcacgcc gacctcgccg cggtagtcgg cgtcgatcgt cccgacgccg
     1681 ttcgcgagcg cgagcccctt cttcagcgcg aggccggagc gggcgcgcac ctgcgcctcg
     1741 aagccgggcg ggatctcgag cgtcaggccg gtcgggacga cggcgcgcgc gccgggggcg
     1801 agcgtcagcg gctcctcgag cgccgcgcgc aggtcggccc cggcggcgag gtcgctcgcg
     1861 tagaggggcg ggccgaggtc cgcgcactcc gcgcggagcc ggaagcggac cgtgacgctc
     1921 gcgccggggg tcacgccgcg agctcgcccg cgaggtcgtc gacggtggcg ccgacgagcg
     1981 tggggccggt gacgccgaag ccggaggcgt cgggctcccc gaagagggcg gcgatcgcgt
     2041 cgtcgacgtc ggcggaagtg acggcctcca tcgtggcgat cgtctcgtcg gcctcccagg
     2101 gacgcccgcg atagaaccag ccgcgaacgg ccgactcgcg ccgcgaggag gtcgactcga
     2161 gcgacatcgc gacctcggcg cggacctggt ccttcgcccg gcggagctcg gcgcgcgtga
     2221 cgccggtctg ccggagctcg cggaggaccg cgccggtcgc ccggacgagc ttcgggagct
     2281 tcttcggcgc ggtcgccgcc tcgatgagcg ccatcccccc gtcccggtgg agcgagaggc
     2341 ccgcccccac ctggtaggcg aggccgtgcc gctcgcggac gcgccgccag aggcgcgagg
     2401 agacgccgcc gccgaggacg acggagagga gccacgccgc ggggacgagc cggttcgcgt
     2461 aggcgggcgc cggggtgccg acgaggaggt gcgtctggtt caggtcgggc ctcgggatgt
     2521 gaaaggcgca gcggttcccg cgcggggagg gcccggcctg gcggacgggg ccgagcggag
     2581 ccgcggcgtg ccggcgcctg cgccgctcgg tcgagaggag cttcaggaac gcctccggct
     2641 cgaacgcgcc gacggcgacg aggagcgtcc gttcggggcg gaagacctcg cggaagcggc
     2701 ggacgagccg ggcgcgcgtg agcgcgccga ccgtctcctc cgtcccgagg atcggggcgc
     2761 cgagcggatg gtcgggccag taggagcgga cgaacgtctc gtggaggcgg tcctccggga
     2821 cgtcgctcgc ctcggccatc tcctcgagga tcaccttccg ctccgtctcg acgtcgtcgg
     2881 cggtgaaggc gggggcctcc gtcaggtcga ggaggagcga gagcgcgtcg gggaggcgct
     2941 cggacggggt ccgggcgtgg acggccatcc actccttcgt cgtgtaggcg tcgcagtcgc
     3001 cgccgagccg atcggtcgtc cgggcgatgt cggcgcccgt ccggcgccgg gcacgccgga
     3061 agaggaggtg ctcgaggaaa tgggtcgagc cgaggtcgga ggcctcctcg tcggccgagc
     3121 ctcccgccac gtaaacgccg agcgcgacgc ttcgcacgtc gcgcatcggg tcgagccaga
     3181 cggtcaggcc gtcgccgagg gtccgcgttt ccacggcgcg gatcttaccc gcgggccgtc
     3241 ggcgtcacgg cgtcccgccg gaaccgccgg gcggcggcat cggcgcccct ccgacgccgg
     3301 ggcccggccc tccgcccgag ctgatgcccg gagccccgcc cgcgaggctc gcctccgtga
     3361 acttccactc gttgtaggcc tcgcgccc
//
LOCUS       DAOTEZ010000459         3381 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_21388_length_3381_cov_6.101624, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000459 DAOTEZ010000000
VERSION     DAOTEZ010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21388_length_3381_cov_6.101624"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="P5164_17795"
     CDS             <1..415
                     /locus_tag="P5164_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005036861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY45788.1"
                     /translation="AECLALVRLDGIEGKRPSELSGGMRRRVGFARAIALKPEILLFD
                     EPTTGLDPITTDALARVMIRLRDRLNPTMVTITHDLKVAFAVAERVALLDAGKIQADL
                     PPSEFEASDDPRVAAFVRGDGSEDAGPARREEVRE"
     gene            412..1854
                     /locus_tag="P5164_17800"
     CDS             412..1854
                     /locus_tag="P5164_17800"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRY45789.1"
                     /translation="MNATAKIGLFAMAVLIALAVLVLKIEDLPFGGRARAATAEAVFK
                     DVAGLDDKSTVRIAGVRVGRIDGIRLLPDGTAVARLLFDSDVELREGAYGQVKNLGLL
                     GDKYVDLYAGDPAKPVLPKGARIPGSVPVEFDQLTKLAEEIGNDVKQLTGALSSSLGG
                     ETGEAKINRIVDNVGALAEELRALVAENRANVDVTVANLKEFSSSIRETLARLDRILD
                     ENRPGVKGSVENIEELSAKLKTSADNLSSITGKIDKGEGTLGKLVNDETSHKNLNEAL
                     TSIKTGVEELRTTLTRINRIELDLGFYGEYATRTEESKGAFRIDVTPREGKFYRVELL
                     GLPNGRRYDETYRYETSVDGGPPSVVTTKVTRTEDEFGLSAQLGYRFKDTTLRAGLIE
                     SRGGFAIDQHLLKDRLQLTAEAWDFSRDDGDAQVKLYGQWQSRGAMFFRAGVTDVINP
                     AGRSFFLGAGLRWKDEDIKTLLPAAIGMLP"
     gene            1854..3248
                     /gene="radA"
                     /locus_tag="P5164_17805"
     CDS             1854..3248
                     /gene="radA"
                     /locus_tag="P5164_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRY45790.1"
                     /translation="MAKRAGRVFTCTACDAQSPRWLGRCPECGGWNTFVEGEPERPVR
                     PGERAPAAAGSTVVRMKDVDAADTPRLLTGLSDVDRVLGGGLVPGSVVLLGGEPGIGK
                     STLLLQVAARLAGLAGEVLYVSAEESARQVRLRADRLGAVVDGLHLLAETSVDRVLEA
                     AASRPWAAVVVDSIQTVSSPDLPSTPGSVSQVRDGAARLSVFAKTGGTPVVLVGHVTK
                     DGTLAGPKTLEHLVDTVLSFEGERFQAHRVLRALKNRFGPVHELGVFEMTGTGLVEVT
                     NPSALYLGGRTGGRPGSAILAAVEGTRPLLLEVQALTVPVKYGTPRRTAIGFDGTRLA
                     LLLAVLERHGGLPMSGHDVFLNIAGGAESEEPAADLAVLAAVAGSARETALPKGAVAF
                     GEVGLLGEVRAVTDATLRLKEAVSLGFTEAFLPAGNAAEAAAFPDLGVTPVASVEELL
                     ARTAVRPAGPGRRP"
BASE COUNT          490 a         1154 c         1277 g          460 t
ORIGIN      
        1 cgcggagtgc ctggcgctcg tccggctcga cgggatcgag gggaagcgcc cgtcggagct
       61 ctcggggggg atgcgccgcc gcgtcggctt cgcgcgggcg atcgcgctga agccggagat
      121 cctgctcttc gacgagccga cgacggggct cgacccgatc acgacggacg ccctggcccg
      181 cgtgatgatc cggctccggg accggctgaa cccgacgatg gtcacgatca cgcacgacct
      241 gaaggtcgcc ttcgccgtgg cggagcgcgt cgcccttctc gacgccggga agatccaggc
      301 ggacctcccg ccgtcggagt tcgaggcgtc ggacgacccg cgggtcgcgg cattcgtacg
      361 cggggacggg agcgaggacg cgggtcccgc ccgccgcgag gaggttcggg aatgaacgcg
      421 accgccaaga tcgggctctt cgcgatggcc gtcctgatcg ccctggccgt cctcgtcctg
      481 aagatcgagg acctcccctt cggcggtcgg gcgagagcgg cgaccgccga ggccgtcttc
      541 aaggacgtgg ccgggctgga cgacaagtcg acggtgcgaa tcgcgggcgt gcgggtcggc
      601 cgcatcgacg ggatccgcct cctccccgac gggacggcgg tggcgcggct gctgttcgac
      661 tccgacgtcg agctgcgcga gggcgcctac ggccaggtga agaacctggg cctcctcgga
      721 gacaagtacg tcgacctcta cgcgggcgac ccggcgaagc cggtcctgcc gaagggggcg
      781 cggatccccg gctcggtccc ggtcgagttc gaccagctga cgaagctcgc ggaggagatc
      841 gggaacgacg tcaagcagct gaccggggcg ctctcgtcct cgctcggcgg cgagacgggc
      901 gaggcgaaga tcaaccggat cgtcgacaac gtcggcgcgc tcgccgagga gctgcgggcc
      961 ctggtcgcgg agaaccgggc gaacgtcgac gtgacggtcg cgaacctgaa ggagttctcg
     1021 tcctcgatcc gcgagaccct cgcgcgcctc gaccggatcc tggacgagaa ccgcccgggc
     1081 gtgaagggct cggtcgagaa catcgaggag ctctccgcga agctgaagac ctcggccgac
     1141 aacctcagct ccatcacggg gaagatcgac aagggggagg ggacgctcgg gaagctcgtc
     1201 aacgacgaga cctcccacaa gaacctgaac gaggcgctca cgtcgatcaa gacgggcgtc
     1261 gaggagctcc ggacgacgct gacccggatc aaccggatcg agctcgacct cgggttctac
     1321 ggcgagtacg cgacgcggac ggaggagtcg aagggggcgt tccgcatcga cgtgacgccg
     1381 cgggagggga agttctaccg cgtcgagctg ctcgggcttc cgaacggccg ccgatacgac
     1441 gagacgtacc gctacgagac gagcgtcgac ggcgggccgc cgagcgtcgt gacgacgaag
     1501 gtgacgcgga cggaggacga gttcggcctc tcggcccagc tcggctaccg gttcaaggac
     1561 acgacgctcc gggccggcct catcgagtcg cgcgggggct tcgcgatcga ccagcacctc
     1621 ctgaaggacc ggctccagct caccgcggag gcgtgggact tcagccgcga cgacggggac
     1681 gcgcaggtga agctctacgg ccagtggcag agccgcggcg cgatgttctt ccgcgccggg
     1741 gtcaccgacg tcatcaaccc ggcggggcgt tcgttcttcc tcggcgcggg gctccgctgg
     1801 aaggacgagg acatcaagac gctcctcccg gccgcgatcg gaatgcttcc ctgatggcga
     1861 agcgcgcggg gcgcgtcttc acgtgcacgg cgtgcgacgc ccagagcccg cgctggctcg
     1921 gccgctgtcc ggagtgcggc ggctggaaca cgttcgtcga aggagagccg gagcgaccgg
     1981 tgcgtccggg ggagagggct ccggccgccg cgggctccac cgtcgtccgg atgaaggacg
     2041 tcgacgccgc ggacacgccg cgcctcctga cgggcctctc cgacgtcgac cgggtcctcg
     2101 gcggaggcct cgtcccgggc tccgtcgtcc tcctcggggg ggagccgggg atcggcaagt
     2161 cgacgctcct gctgcaggtc gccgcccgcc tggccgggct tgccggggag gtgctctacg
     2221 tctcggccga ggagtcggcg cggcaggtcc gcctgcgcgc ggatcgcctc ggcgcggtcg
     2281 tggacggcct ccatctcctc gcggagacct ccgtcgaccg ggtcctcgag gcggcggcgt
     2341 cgcggccctg ggccgccgtc gtcgtcgact cgatccagac cgtctcctcg cccgacctgc
     2401 cgtcgacgcc gggctccgtg tcgcaggtgc gggacggggc ggcgcggctc tccgtcttcg
     2461 cgaagacggg cgggacgccg gtcgtcctcg tcgggcacgt cacgaaggac ggcacgctcg
     2521 ccgggccgaa gaccctcgag cacctcgtcg acacggtcct ttcgttcgag ggggagcgct
     2581 ttcaggccca cagggtgctg cgcgcgctga agaacaggtt cggccccgtc cacgagctcg
     2641 gcgtcttcga gatgacgggg accggtctcg tggaggtgac gaatccctcg gcgctctacc
     2701 tcggaggccg cacgggcggg cggcccggct cggcgatcct cgcggcggtg gaagggacgc
     2761 ggccgcttct cctcgaggtt caggcgctga cggtgccggt gaagtacggc acgccgcggc
     2821 gaacggcaat cggcttcgac ggcacccgtc tcgctctcct cctggccgtc ctcgagaggc
     2881 acggcggtct cccgatgtcg gggcacgacg tcttcctgaa catcgccgga ggcgcagaga
     2941 gcgaggagcc cgcggcggac ctcgccgtcc tcgccgcggt cgcggggagc gctcgggaga
     3001 cggcgctgcc gaaaggggcc gtcgcgttcg gcgaggtggg gctcctcggc gaggtccggg
     3061 ccgtcacgga cgcgacgctg cgtctgaaag aggccgtgag cctcggcttc acggaagcgt
     3121 tcctccccgc ggggaacgcg gcggaggccg cggccttccc ggacctcggc gtgacgccgg
     3181 tcgcgagcgt cgaggagctc ctcgcgcgga cggccgtcag gccggcagga ccgggtcgac
     3241 gcccgtgagc gtgcggtacg cctcgacgta gcgcgcgagc gtgccggcga cgacgttcgg
     3301 cggtaggagc ggggcgggcg gcgtcttgcc ccagccggtc gtctcgagcc agtccctcac
     3361 gaactgcttg tcgtaggacg g
//
LOCUS       DAOTEZ010000460         3367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_21509_length_3367_cov_7.312500, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000460 DAOTEZ010000000
VERSION     DAOTEZ010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21509_length_3367_cov_7.312500"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="P5164_17810"
     CDS             complement(<1..1019)
                     /locus_tag="P5164_17810"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRY45791.1"
                     /translation="MATLPRSIRDLELSSRRVFLRVDFNVPIKEGVVRDDTRIAEALP
                     TIRLAREKGARLILASHLGKAKGTPDPKYSLAPVAKKLEELLGAPVAFAADCVGEAAE
                     TAAKGLADGGVLLLENTRFHAGEEGNDPAFAKALASLAEVYVNDAFGTAHRAHASTAG
                     MAVHFAPGARGVGLLMEKELNALGKVVDDIDRPFVGILGGAKILGKIAALEALVERAD
                     TLLVGGGMANHFVVALGLGVGKSLLEPEGVPIARKVIDRCKERSVNLVLPSDFLVASS
                     LEAAAQAKAVAMNAIPADQAALDIGPKTLEMFARMTKGAKTIFWNGPMGVFETKPFDA
                     GTRGVA"
     gene            1105..2202
                     /gene="mtnA"
                     /locus_tag="P5164_17815"
     CDS             1105..2202
                     /gene="mtnA"
                     /locus_tag="P5164_17815"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HRY45792.1"
                     /translation="MTKTPLGTRVTPIDAAPGRLRLLDQRRLPREEAWIDCGSADAAA
                     EAIRSLAVRGAPLIGVAAAWGLAAEARRLGGAGGSFDATWEAAAARLEKARPTAVNLR
                     SGVERMRGVFRSAAGVPAAERVARLEAEAARFEAEDREACAAMGRHGAEWVAARLGGD
                     PKAPLRVLTHCNAGALATAGIGTALGVVRALAAVRPVAVFADETRPFWQGARLTAWEL
                     AKDGIDVTILPDVAAASVIASGKVAVAVVGADRIAANGDVANKVGTYGVALACRAHGV
                     PFVVAAPRTTLDPACPSGRDIPIEWRDGAEVLLLDPSGETPLSVAPEGVPALYPAFDV
                     TPAELVDAIVTEAGVSTAPHAEGLARLLALP"
     gene            2199..3305
                     /gene="tsaD"
                     /locus_tag="P5164_17820"
     CDS             2199..3305
                     /gene="tsaD"
                     /locus_tag="P5164_17820"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRY45793.1"
                     /translation="MSYVLGLETSCDETSVALLREDGSIVASVVSSQIDMHRRYGGVV
                     PEIAARAHLTNLPPLLDEALERAGARLEEVGLVVATAGPGLVGALLVGLAEGRGIALG
                     LGVPFVPGHHIEGHALSPFLDLDGGPARAVPARFAALVVSGGHSHLFSFSPSGIVVLA
                     KTRDDAAGEAFDKVAKMAGLGYPGGPVVDRLARSGRVVEPFAVPRFKAGAHDLSFSGL
                     KTAVRDRLAALGFSPNTGVALRGPALAEEDAPGALCDLLAAFQEAVVAQLEDRLERLH
                     ETERFPLLTVSGGVAANSLLRERLAAWGKRRGVEVLLAPRALTSDNAAMVAFAGLLRH
                     RAGAPHEGFSAPARSRWPLEGMPWAPPAGTPPFR"
BASE COUNT          392 a         1285 c         1217 g          473 t
ORIGIN      
        1 gcgacgccgc gcgtcccggc gtcgaacggc ttcgtctcga agacccccat cggaccgttc
       61 cagaagatcg tcttcgcccc cttcgtcatc cgggcgaaca tctcgagcgt cttcggaccg
      121 atgtcgagcg ccgcctggtc ggcggggatc gcgttcatcg cgaccgcctt cgcctgggcc
      181 gccgcctcga gcgaggaggc gacgaggaag tcggagggaa ggacgaggtt cacgctccgc
      241 tccttgcagc ggtcgatgac cttccgggcg atcgggaccc cctcgggctc caggagcgac
      301 ttcccgaccc cgaggccgag cgcgacgacg aagtggttcg ccatcccgcc cccgacgagg
      361 agcgtgtcgg cccgctcgac gagcgcctcg agggccgcga tcttcccgag gatcttcgcg
      421 ccgccgagga tgccgacgaa cggccggtcg atgtcgtcga cgactttccc gagcgcgttc
      481 agctcctttt ccatcaggag gccgacgccg cgggcgccgg gagcgaagtg gaccgccatg
      541 ccggccgtcg aggcgtgggc gcggtgcgcc gtgccgaacg cgtcgttcac gtagacctcg
      601 gcgagggagg cgagggcctt cgcgaaggcg gggtcgtttc cctcttcccc ggcgtggaag
      661 cgggtgttct cgaggaggag gacgccgccg tcggcgagcc ccttcgcggc cgtctcggcc
      721 gcctcgccga cgcagtcggc ggcgaaggcc accggtgcgc ccaggagctc ctccagcttc
      781 ttcgcgacgg gggcgagcga gtacttcggg tcgggggtcc ccttcgcctt gccgagatgc
      841 gaggcgagga tgagccgcgc ccccttctca cgggcgaggc ggatcgtcgg gagcgcctcg
      901 gcgatccggg tgtcgtccct gacgaccccc tccttgatcg ggacgttgaa gtcgacgcgg
      961 aggaagacgc ggcgggagga gagctcgagg tctcggatgc tgcgggggag cgtggccatc
     1021 ggaagactcc tcgggtccgt ccgctttggc gcgagccggg acggaccggc acctcgtggc
     1081 gaggatcgat tctatagtcc gcgcgtgacg aagacccctc tcggaacgcg ggtgacgccg
     1141 atcgacgccg cgcccggccg cctccgcctc ctcgaccaga gacgcctccc gcgggaggag
     1201 gcgtggatcg actgcggctc cgccgacgcg gcggccgagg cgatccgaag cctcgccgtc
     1261 cgcggcgcgc ctctcatcgg cgtcgccgcg gcgtgggggc tcgcggcgga ggcgcggcgg
     1321 ctcggggggg ccggcgggag cttcgacgcc acgtgggaag ccgccgcggc gcgcctcgag
     1381 aaggctcggc cgaccgccgt gaacctccgc tccggcgtgg agcggatgcg tggggtcttc
     1441 cgctcggcgg cgggcgtgcc cgccgccgag cgcgtcgcgc gcctcgaggc cgaggcggcc
     1501 cgcttcgagg cggaggaccg cgaggcctgc gccgcgatgg ggaggcacgg ggccgagtgg
     1561 gtcgccgccc ggctcggcgg cgacccgaag gcccccctcc gggtcctgac ccactgcaac
     1621 gccggcgccc tcgcgacggc gggcatcggg acggcgctcg gcgtcgtccg ggcgctcgcg
     1681 gccgttcgcc ccgtcgccgt cttcgccgac gagacccgcc ccttctggca gggggcgcgc
     1741 ctcaccgcgt gggagctcgc gaaggacggg atcgacgtga cgatcctccc cgacgtcgcc
     1801 gcggcctcgg tgatcgcctc ggggaaggtc gccgtcgccg tcgtcggcgc cgaccggatc
     1861 gcggcgaacg gcgacgtcgc gaacaaggtc ggcacctacg gcgtcgcgct cgcctgccgg
     1921 gcccatggcg tccccttcgt cgtcgcggcc ccccggacca cgctcgaccc cgcctgcccc
     1981 tcggggcgcg acatcccgat cgaatggcgc gacggcgccg aggtcctcct cctcgacccg
     2041 tcgggcgaga ccccgctctc cgtcgcgccc gagggcgtcc cggccctcta tcccgccttc
     2101 gacgtgacgc ccgcggagct cgtcgatgcg atcgtcacgg aggcgggcgt ctcgaccgcg
     2161 ccgcacgccg aagggctcgc gcgccttctc gccctcccgt gagctacgtc ctcggcctcg
     2221 agacctcctg cgacgagacc tcggtcgccc ttctccgcga ggacggatcg atcgtcgcgt
     2281 ccgtcgtctc gtcgcagatc gacatgcacc gccgctacgg cggcgtcgtc cccgagatcg
     2341 ccgcccgggc gcacctgacg aacctcccgc ccctcctcga cgaggcgctc gagcgggccg
     2401 gagcacgcct cgaggaggtc ggcctcgtcg tcgcgacggc gggtccgggg ctcgtcggcg
     2461 ccctcctcgt cggcctcgcg gagggacgcg ggatcgcgct cggcctcggc gtcccgttcg
     2521 tcccggggca ccatatcgag gggcatgccc tctcgccctt cctcgacctc gacggcggcc
     2581 cggcgcgggc cgtccccgcg cgcttcgcgg ccctcgtcgt gtcggggggc cactcccatc
     2641 tcttctcctt ctcaccgtcg gggatcgtgg tcctcgcgaa gacgcgggac gacgcggcgg
     2701 gcgaggcttt cgacaaggtc gcgaagatgg ccgggctcgg ctacccgggc gggcccgtcg
     2761 tcgatcgcct ggcccggagc ggccgtgtcg tcgagccgtt cgccgtcccc cgcttcaagg
     2821 cgggcgcgca cgacctctcc ttctccggtc tgaagacggc cgtgcgcgac cggctcgccg
     2881 ccctcggctt ctcgccgaac accggcgtcg ccctccgggg cccggccctc gcggaggagg
     2941 acgcgccggg agcgctctgc gacctcctcg ccgcgttcca ggaggccgtc gtcgcccagc
     3001 tcgaggaccg gctcgagagg ctccacgaga cggagcgctt cccgctcctg acggtctccg
     3061 gaggcgtcgc ggcgaactcc ctgctccgcg aacggctcgc ggcgtggggg aaacggcgcg
     3121 gcgtggaggt cctcctcgct ccgcgggcgc tcacctccga caacgccgcg atggtcgcct
     3181 tcgccggcct cctgcggcat cgcgcgggag ctccccacga agggttctcc gctcccgccc
     3241 gctcccgctg gccgctggag gggatgccgt gggccccacc ggccgggacg ccgccgttcc
     3301 gctagaatag aaggttcggg cgcgaggccc gactcacacc atgcccgatc cctcccgcat
     3361 ccgaaat
//
LOCUS       DAOTEZ010000461         3356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_21615_length_3356_cov_3.914571, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000461 DAOTEZ010000000
VERSION     DAOTEZ010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21615_length_3356_cov_3.914571"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..752)
                     /locus_tag="P5164_17825"
     CDS             complement(231..752)
                     /locus_tag="P5164_17825"
                     /inference="COORDINATES: protein motif:HMM:NF025710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HRY45794.1"
                     /translation="MNEKSFNGRTGWTVAAIALALVATASLSNPAQAAATASASIGVR
                     ILAPADVAIASGAVAVSGLSSLEWTGTPELRNGQAVAVNVSPRRADSGAAQWLVRGGR
                     NAARTMQVPSEAVVRNGDAELTVSGLTLQSGDARPELGGAVAVALGGNVEIGAGAAGG
                     SYVGNVLVVTAWE"
     gene            complement(887..>3356)
                     /locus_tag="P5164_17830"
     CDS             complement(887..>3356)
                     /locus_tag="P5164_17830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45795.1"
                     /translation="GELARLLALAVEVDVARGTAEGFVLAENRRFSLRAREASVTVSG
                     EESRFDRTRVEVHEDDLYADVAFLSKWFPVDLLVDPYSARLTVRPREPLPVQQRAERE
                     RVAARLATARRAPEGFPDAPNPHRLASVPSIDANLRLLVSPDDGGGQRLGFQHSALLT
                     GDLLGAEASLFLNGNDSEAVSDAWGSLARRDPEGRLLGKLGLREAVLGEVASPGFELV
                     SDAQTGPGLLLSSFPLQRPAEWGRTSLSGPLLPGWEVELYGNDSLLGYQASRADGLYE
                     FADVPLLPGANELRLVFYGPQGQRQEEVRRINAAETFTPTGELRWRAVANDPRQVGRR
                     GHLLAEYGLSKGLSVSGGVAGLRTERGRELGYGRLGAQLLRGILLARVDGAVSGGSGT
                     AVRGELQARTATFGFSAAYSLLDGFESEVYRPEFGTVSSRLELRFDVRPHLGAGIRLP
                     ISGQLRRDSLEGGGDLVRATGQLSTNVSRLYVSSLWSWSRVRRPGSSGEAIGFGTVLV
                     SRLVRRTSLRGELQYDFDPGTSLRSAGLNAEFPIGPRLLGRGGLTYLRREGETLLFAG
                     LSRRTGVVGVSFEASWGRTSRLNAALSVGIGLGRDPRSGRFHAQAPPFAALGAASARV
                     FLDTDGDGAWNAGEPPVEGAGFFVNRGSVRTTTGPDGLALLPTLPTLARTEVGLSTAT
                     LEDPLWVAKQRGWGFVPRPGVVTELDFPVTVTGEITGTIRTRRGEQERESAGIRLQLV
                     SPGGEVVAETRSAYDGFYDLPRIPPGRYVLRVDPAQVERLGLSGELEKAVEILPSGSI
                     LDGVDLLLEVRPAAPVAPRAEDPA"
BASE COUNT          474 a         1238 c         1237 g          407 t
ORIGIN      
        1 cgtcgtcccg ctcccgccgg cccgccaggc gatcatcgac gccggcggcc tcatcgccta
       61 cacgcggaag aagatgctgg aggcgcaggc gaggggctga gcgtcccgct gtcccgcccg
      121 ccccgagacc cgaagggccg cgcgatcgcg cggcccttcg ttcgtccgtg tcgccccccg
      181 tgccgaaggg gcccgtctcg cccggggatc gcgggaaccc tcctctcgtc ttactcccag
      241 gcggtgacga cgaggacgtt gccgacgtag gagccgccgg ccgcgccggc gccgatctcg
      301 acgttgccgc cgagcgcgac ggcgaccgcg ccgccgagct ccggccgcgc gtcgcccgac
      361 tgcagcgtga ggccgctgac cgtgagctcg gcgtcgccgt tgcggacgac cgcttcgctc
      421 gggacctgca tggtgcgggc ggcgttgcgg ccgccgcgga cgagccactg cgcggcgccg
      481 gagtccgccc tgcgggggga gacgttgacc gcgacggcct gcccgttcct aagctcggga
      541 gtgccggtcc actcgaggga ggagaggccg gagacggcga ccgcgcccga ggcgatcgcg
      601 acgtcggccg gggcgaggat ccggacgccg atcgaggccg aggcggtggc ggcggcctgc
      661 gccgggttcg agagagaagc cgtggcgacg agagcgagag cgatcgctgc gaccgtccaa
      721 cccgtcctgc cgttgaagct cttctcgttc atgcctcccg tgattgcagg actccggcca
      781 gagcacagga caccacaaaa ccattgttat caatcattta agaatcggat gccacctccg
      841 ctccgtctcc ggacacaccc ctggtgtgtc acggacacac gcgggatcag gccgggtctt
      901 cggcccgggg cgcgaccggg gcggccggcc ggacctcgag gaggagatcg acgccgtcga
      961 ggatcgagcc ggaggggagg atctcgaccg ccttctcgag ctctcccgag agcccgagcc
     1021 tctcgacctg cgccgggtcg accctcagga cgtagcggcc cgggggaatt ctcggcaggt
     1081 cgtagaagcc gtcgtaggcg ctccgcgtct cggcgacgac ctctccgccg ggcgagacga
     1141 gctggaggcg aattccggcc gactcgcgct cctgctcccc gcggcgggtc cggatcgtcc
     1201 cggtgatctc ccccgtgacc gtcaccggga agtcgagctc ggtcacgacg ccgggcctcg
     1261 ggacgaagcc ccagccgcgc tgcttcgcca cccagagcgg gtcctcgaga gtcgcggtcg
     1321 aaagtcccac ctccgtccgc gcgagggtcg gcagggtggg gaggagcgcc agcccgtccg
     1381 gaccggtcgt cgtccggacg ctcccgcggt tgacgaagaa gcccgcccct tcgaccggcg
     1441 gctcgccggc gttccacgcc ccgtccccgt ccgtgtcgag gaagacccgg gccgacgcgg
     1501 cgccgagtgc ggcgaacggg ggggcctgcg cgtggaaccg cccgctccgc gggtcccggc
     1561 cgaggccgat cccgacggag agcgccgcgt tgagccggga cgtgcgcccc cagctcgcct
     1621 cgaacgagac gccgacgact cccgtccggc ggctgaggcc ggcgaagagg agcgtctccc
     1681 cttcgcgtcg gaggtacgtg aggccgccgc ggccgagcag ccgcgggccg atcgggaact
     1741 ccgcgttcag gcccgcggat cggagggagg tcccggggtc gaagtcgtac tggagctcgc
     1801 cgcgaaggct cgtccgccgg acgaggcggc tcacgaggac cgtcccgaac ccgatcgcct
     1861 ccccggacga gcccggcctc cggacgcgcg accagctcca gagggacgag acgtagaggc
     1921 gggagacgtt cgtcgagagc tgtccggtcg cgcggacgag atcgccaccc ccctcgaggg
     1981 aatcccgccg gagctgcccg gagatcggga gccggatgcc cgcgccgagg tggggacgga
     2041 cgtcgaaccg gagctccagc cggctcgaga ccgtgccgaa ctccggccgg tagacctcgc
     2101 tctcgaaccc gtcgagcagc gagtaggccg ccgagaatcc gaacgtcgcc gtccgggcct
     2161 gcagctcgcc gcggaccgcg gtcccgctcc cgccggagac cgcgccgtcc acgcgggcga
     2221 ggagaattcc gcggaggagc tgggcgccca gccggccgta cccgagctcc cgtccgcgtt
     2281 cggtccgcag gccggcgacc ccgcccgaca cggagagccc cttcgaaagg ccgtactccg
     2341 ccaggaggtg gccgcgccgg cccacctgcc gcgggtcgtt cgcgacggcc ctccagcgga
     2401 gctccccggt cggcgtgaac gtctcggccg cgttgatccg cctcacctct tcctggcgct
     2461 ggccctgcgg accgtagaag acgaggcgca gctcgttcgc gcccgggagg agcgggacgt
     2521 cggcaaactc gtagaggccg tcggcgcgcg aggcctggta gccgaggagc gagtcgttcc
     2581 cgtagagctc gacctcccac ccggggagga gcggccccga gagcgaggtc cggccccact
     2641 cggccggccg ctgcagcggg aagctcgaca ggagcagccc cgggcccgtc tgcgcgtccg
     2701 agacgagctc gaagcccggc gacgcgacct cgcccaggac cgcctccctc aggccgagct
     2761 tcccgagaag cctcccctcc gggtcgcgcc gggcgaggga gccccaggcg tcggagaccg
     2821 cctcgctgtc gttgccgttc aggaagaggc tcgcctcggc cccgaggagg tcgcccgtca
     2881 ggagggcgga gtgctggaag ccgagccgct gccctccccc gtcgtccggc gagacgagga
     2941 ggcggaggtt cgcgtcgatg gacggtacgg aggcgaggcg gtgggggttc ggcgcgtcgg
     3001 ggaacccttc cggcgcgcgc cgggcggtcg cgagccgggc cgcgactctc tcccgctcgg
     3061 ctctctgctg gaccggcagc ggctcgcgcg ggcgaacggt gaggcgcgcg gagtaggggt
     3121 cgacgagaag gtcgaccggg aaccacttcg agaggaacgc gacgtcggcg tagaggtcgt
     3181 cctcgtggac ctcgacccgc gtcctgtcga accggctctc ctcgccggag accgtgacgg
     3241 acgcctccct cgcacggagc gagaaccgcc ggttctccgc gaggacgaac ccctccgccg
     3301 tgccgcgcgc cacgtccacc tccacggcga gggcgaggag gcgggcgagc tcaccc
//
LOCUS       DAOTEZ010000462         3348 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_21695_length_3348_cov_7.227756, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000462 DAOTEZ010000000
VERSION     DAOTEZ010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21695_length_3348_cov_7.227756"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2663
                     /locus_tag="P5164_17835"
     CDS             <1..2663
                     /locus_tag="P5164_17835"
                     /inference="COORDINATES: protein motif:HMM:NF026115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyketide synthase dehydratase
                     domain-containing protein"
                     /protein_id="HRY45796.1"
                     /translation="PVYEMKTVFGFFPPAALASQAGLPVPDAQRELLERPGNVLVDLT
                     TRAAAFFDPRRPRIAGPMLRMIDRVDGLWPGAGKAGLGQLRAVKDVDAGEWFFKAHFF
                     QDPVQPGSLGIEAMIQALQLFMLEAGMDDGIVAPRFEPLATGADLTWKYRGQVLPHHR
                     AVHTTIEITAKGRDERGAWAICEASLWADGQRIYEATNLGMRIVPGNPADAPRTNEIV
                     LDPESDRWLLDHRPTWTVPALPMMSIVDLLARGSTGDGPVTGLRDVRVGGWFLVDEPR
                     RLRTEESGGVVRLLAPTPEGEKEVARARVVRGEYAPRPEPLPEARGADVPLPYETGEL
                     FHGPAFQVAERLVMGKGESSITLRAESGVPVGLLNPGLLDGATHGIPHAELHGWNDAS
                     DPGKVAFPAFIPEIDFFGPTPVAGSIRCEVRQQPYLGSPDYPVFRIQLVGPDGVWCQL
                     RLVESCFPKGPLGSTDPASRRAFLRDRTPVEDLRLSRVENGATVLTDADVAATDWLPG
                     TVAAVYGSRETEEIARAEHAAAAHALHPGRVFAQLPLTRFDLAAERAGGEVRVSGDGR
                     GRLDLTPVRRFWTRWFDRGPWPVEDLYYGLVGRFVGRVVVGDPAAYAALRGRSVLYLA
                     NHQTGVESLLFSIVASALNEVPTVTLAKIEHRETWLGRLIALSFAYPGVADPRLMTFF
                     DREDKASLPRVIGELAAEMKGPGRSVMVHVEGTRSLTCRKPVEKMSGAFVDMALAVGA
                     PIVPVRFVGGLPAEPLEKRLEFPLGMGRQDIHLGEPLLPERLAGLHYGARKALVVAAI
                     NALGVPNALEQPYPGDPDFERRVNAWQAARDVSHEHATLREVLAELDSPGDEVRRLLA
                     PPNAAALAGDETPAGRWLRELGRRLLGEGP"
     gene            complement(2660..>3348)
                     /locus_tag="P5164_17840"
     CDS             complement(2660..>3348)
                     /locus_tag="P5164_17840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HRY45797.1"
                     /translation="VADDVGYLNSLGRKQTAALDKSARVAEAQAHAEASVREAENNLS
                     ARLALVDAEGAIAQAEAEKRITQCVTGQEAMIAEEVGKVTAAIAKAEAELKVQKARLE
                     QVRLKLEADVVAPARADMEKKQAEARGRAAKIVEDGRATAAVLTQMIETWKAAGPNAR
                     DIFLMQKLQSVMSSLVETIQSVKVDRVAYLPASAKVAQGVQLVEQVKAAFGVDLAKVV
                     GRLGAPEEKG"
BASE COUNT          418 a         1249 c         1204 g          477 t
ORIGIN      
        1 ggccggtcta cgagatgaag accgtcttcg gcttcttccc gccggccgcg ctcgcgagcc
       61 aggccgggct cccggtcccc gacgcgcagc gcgagctgct ggagcggccg gggaacgtcc
      121 tcgtcgacct gacgacgcgg gccgccgcgt tcttcgaccc gcgccggccg cggatcgcgg
      181 ggccgatgct ccggatgatc gaccgcgtcg acggcctctg gcccggggcg gggaaggcgg
      241 gcctcggcca gctgcgcgcc gtcaaggacg tcgacgcggg cgagtggttc ttcaaggccc
      301 acttcttcca ggacccggtg cagcccggct ccctcgggat cgaggcgatg atccaggcgc
      361 tgcagctctt catgctcgag gcgggaatgg acgacgggat cgtcgcgccc cgcttcgaac
      421 cgctcgcgac cggtgcggac ctcacctgga agtaccgcgg gcaggtcctc ccgcaccacc
      481 gggccgtcca cacgacgatc gagatcacgg cgaaggggcg cgacgagcgg ggcgcctggg
      541 ccatctgcga ggcgagcctc tgggccgacg ggcagcggat ctacgaggcg acgaacctcg
      601 gcatgcggat cgtcccgggc aacccggcgg acgcgccgcg gacgaacgag atcgtcctcg
      661 acccggagag cgaccgctgg ctcctcgacc accgtccgac ctggaccgtg ccggcgctcc
      721 cgatgatgag catcgtcgac ctcctcgccc gaggctccac gggcgacggc cccgtgaccg
      781 gcctgcgcga cgtgcgcgtc ggcggctggt tcctcgtgga cgagccgcgg cgcctccgca
      841 ccgaggagtc gggcggggtc gtccggctcc tcgcgccgac gccggaaggg gagaaggagg
      901 tcgcgcgggc ccgcgtcgtt cgcggcgagt acgcgccccg ccccgagccg ctccccgagg
      961 cgcgcggcgc ggacgtcccg ctcccgtacg agacgggcga gctgtttcac ggacccgcgt
     1021 tccaggtcgc cgagcgcctc gtgatgggga agggcgagtc gtcgatcacg ctgcgcgccg
     1081 agagcggcgt tcccgtcggt ctcctgaacc cgggcctcct cgacggtgcg acgcacggca
     1141 tcccgcacgc ggagctccac ggatggaacg acgcctccga cccgggcaag gtcgcgttcc
     1201 ccgcgttcat tcccgagatc gacttcttcg gcccgacgcc tgtcgccgga agcatccggt
     1261 gcgaggtccg gcagcagcca tacctcggca gccccgatta cccggtcttc cggatccagc
     1321 tcgtcggacc ggacggcgtc tggtgccagc tccgcctcgt cgagtcgtgc tttccgaagg
     1381 ggcctctcgg ctcgaccgac ccggcgtcgc gccgcgcctt cctgcgcgac cggacgccgg
     1441 tcgaagacct tcgtctctcg cgcgtcgaga acggcgcgac agtcctgacg gacgcggacg
     1501 tcgcggcgac cgactggctg cccggcaccg tcgcggcggt ctacggctcc cgcgaaacgg
     1561 aggagatcgc ccgcgccgag cacgcggccg cggcgcacgc gctccacccg ggacgggtct
     1621 tcgcgcagct cccgctcacg cgcttcgacc tcgccgccga gcgggcgggc ggcgaggtgc
     1681 gcgtctcggg cgacggccgg ggaaggctcg acctgacgcc cgtccgccgg ttctggacgc
     1741 gctggttcga ccgcgggccg tggcccgtgg aggacctcta ctacggcctc gtcggccgct
     1801 tcgtcgggcg cgtcgtcgtc ggagacccgg cggcgtacgc ggcgctccgc ggccggagcg
     1861 tcctctacct cgcgaaccac cagacgggcg tcgagtcgct cctcttctcg atcgtcgcgt
     1921 cggcgctgaa cgaggtcccg accgtcacgc tcgcgaagat cgagcaccgc gagacctggc
     1981 tcggaaggct catcgcactt tccttcgcct acccgggcgt ggccgacccg cgcctcatga
     2041 ccttcttcga ccgcgaggac aaggcgagcc tcccccgcgt catcggcgag ctcgccgccg
     2101 agatgaaggg gccgggccgg tcggtcatgg tccacgtgga ggggacacgg tccctgacct
     2161 gccgcaagcc ggtcgagaag atgagcggcg cgttcgtcga catggcgctc gcggtcggcg
     2221 ccccgatcgt cccggtgagg ttcgtcgggg gcctgccggc cgagccgctc gagaagcgcc
     2281 tcgagttccc gctcggcatg ggccgccagg acatccacct cggagagccc ctgttgcccg
     2341 agcgcctcgc cggcctccac tacggcgccc gaaaggcgct cgtcgtcgcg gcgatcaacg
     2401 ctctcggcgt cccgaacgcc ctggagcagc cctaccccgg cgaccccgac ttcgagagac
     2461 gcgtgaacgc ctggcaggcc gcgcgcgacg tctcccacga gcacgcgacg ctccgcgagg
     2521 tcctcgcgga gctggactcg ccgggcgacg aggtccggcg gctcctcgcg cccccgaacg
     2581 ccgccgccct cgccggggac gagaccccgg cggggcggtg gctgcgggag ctgggccgga
     2641 ggctcctggg agagggaccc tagcccttct cttccggcgc gccgagcctc ccgacgacct
     2701 tcgcgaggtc gacgccgaag gcggccttga cctgctcgac gagctggacg ccctgcgcga
     2761 ccttcgcgga ggcggggagg taggcgacgc ggtcgacctt gacgctctgg atcgtctcga
     2821 cgagggacga catgacgctc tggagcttct gcatgaggaa gatgtcgcgg gcgttcgggc
     2881 cggcggcctt ccacgtctcg atcatctggg tcaggacggc ggcggtcgcc cggccgtcct
     2941 ccacgatctt cgcggcccgg ccgcgcgcct cggcctgctt cttctccatg tcggcgcggg
     3001 cgggggcgac gacgtcggcc tcgagcttca ggcggacctg ctcgaggcgc gccttctgga
     3061 ccttcagctc ggcctcggcc ttggcgatcg cggcggtcac cttgccgacc tcctcggcga
     3121 tcatcgcctc ctggcccgtc acgcactggg tgatccgctt ctcggcctcg gcctgggcga
     3181 tcgccccctc ggcgtcgacg agggcgaggc gggcggagag gttgttctcg gcctcgcgca
     3241 cggaggcctc ggcgtgggcc tgcgcctcgg cgacgcgggc gctcttgtcg agggcggcgg
     3301 tctgcttgcg gccgagggag ttcaggtagc cgacgtcgtc ggcgacgt
//
LOCUS       DAOTEZ010000463         3333 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_21840_length_3333_cov_6.352349, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000463 DAOTEZ010000000
VERSION     DAOTEZ010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21840_length_3333_cov_6.352349"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..991)
                     /locus_tag="P5164_17845"
     CDS             complement(113..991)
                     /locus_tag="P5164_17845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45798.1"
                     /translation="MITGFNTDIKHGDRVFHVQTEDRGAGNPIVESLVYVGGQILLSK
                     RSPYADLIVDGKVDEPAVRQLMDLQHRRIIEAIRRGRFDGAVSGTPETGPVRAESALS
                     PAAAAAAAAILAGGPASGAIPKAPAPRPPASAALPKPAPASAASRPPTSGIISTTAPS
                     PERSLDQVIIEYLAAEAASERLELNLVKGGELVSGEPVALTVRASTSLTDRPVSGAQI
                     TLRIVSSISPTQVLYRGASDADGLVKVTAALPDIGNGNAALIISATSSLGTQEIKQLI
                     RRNPAAATSGAPRPGA"
     gene            complement(1042..>3333)
                     /gene="uvrA"
                     /locus_tag="P5164_17850"
     CDS             complement(1042..>3333)
                     /gene="uvrA"
                     /locus_tag="P5164_17850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRY45799.1"
                     /translation="ELDKQKKHTIEVIVDRLVVRRDDEAHRRLTDSLETAVRVADGLV
                     TISVVGGKGETLSEETLSTSYACPDCGTSLPEITPRLFSFNTPYGACAACSGLGSVPR
                     VDPDRLVPDPTLSIADGAIALWKKGSSNWRLRQLEQLSKAAKFSMDVPWKKLPAGVRD
                     MILHGSKEQEIKWKWKSEKGEYVWSSSYKGLVPLLTEKYKEVESEEARQELEAFMSLT
                     PCSECGGRRLKAEALAVKVRGQAIDALAEMTLEDAKAFFASFGPEPREREIAGKILKE
                     IEDRLGFLNDVGIGYLSLGRASATLSGGEAQRIRLATQIGSKLMGVLYVLDEPSIGLH
                     PKDNRKLIETLMAMRDLGNTVVVVEHDEETIRAADRVVDLGPGAGVHGGEVVGEGAAD
                     ELGRFPRSLTGKYLSRELTIPVPEERRAGSGKAITVVGARANNLRDVTARFPLGCLTA
                     VTGVSGSGKSTLVNDILFRAAARLLHGAHEKPGEHERIEGLEHVDKVIDIDQSPIGRT
                     PRSNPATYTNLFGPIRDLMAQVPDARMRGYGPGRFSFNVKGGRCEACGGGGQTAIEMH
                     FLPDVYVTCDVCGGRRYNRETLEVRYKGLNVAEVLDLTAEQAAEVFANVPTIRNVAAT
                     LVEVGLGYIKLGQPATTLSGGEAQRVKLARELARRATGRTLYILDEPTTGLHFDDVRK
                     LLDVLHALVDRGNTVIVIEHNLDVVKTADRIVDLGPEGGARGGRVVAEGTPEEVAASA
                     SSATGAWLARTLGAGPAPRRARR"
BASE COUNT          437 a         1265 c         1127 g          504 t
ORIGIN      
        1 ccgaacccag agattaagag tctcttgctc taccaattga gctaacggcc cgtggcgtgc
       61 ggccggccga cccggacgcg ggatggggat tatacggaga gcgcgccgag attcaagccc
      121 ccggccgggg cgctcccgaa gtcgccgcgg ccggattccg gcggatcagc tgcttgatct
      181 cctgggtccc gagggacgag gtggccgaga tgatcagcgc cgcgtttccg ttcccgatgt
      241 cggggagcgc tgccgtcacc ttcacgaggc cgtcggcgtc gctcgcgcct cggtagagga
      301 cctgcgtcgg ggagatgctc gagacgatcc ggagcgtgat ctgcgccccg gagacgggcc
      361 ggtcggtgag cgacgtcgag gcgcggaccg tgagcgccac cggctcgccc gagacgagct
      421 cgcccccctt caccaagttg agctcgaggc gctcggacgc cgcctcggcc gcgaggtact
      481 cgatgatgac ctggtcgagc gagcgctccg ggctcggtgc cgtcgtggag atgatccccg
      541 acgtcggcgg ccgggaggcc gccgacgcgg gggccggctt cggcagcgcg gcggaggcgg
      601 gcgggcgggg cgccggcgcc ttcgggatcg cgcccgacgc gggccctccg gccaggatcg
      661 ccgccgcagc cgccgccgcg gccggggaga gggccgattc cgcccggacc ggtccggtct
      721 ccggcgtccc cgacaccgcc ccgtcgaacc gtccccgccg gatcgcctcg atgatccgcc
      781 ggtgctgcag gtccatcagc tgccggaccg cgggctcgtc caccttcccg tccacgatga
      841 ggtccgcgta ggggcttctc ttcgagagca ggatctgccc gccgacgtag acgagcgact
      901 ccacgatcgg gttcccggcc ccccgatcct ccgtctggac gtggaagacc cggtccccgt
      961 gcttgatgtc ggtgttgaag cccgtaatca cgacagaccc gggattctag cccggacccc
     1021 gcacccccgg cctcgccgcg cctagcggcg cgcgcgcctc ggcgccggtc ccgctcccag
     1081 cgtccgggcg agccaggcgc ccgtggcgga ggacgcggag gccgccacct cctccggggt
     1141 cccctccgcg acgacgcgcc cgccgcgggc gcccccctcc ggccccaggt cgacgatccg
     1201 gtcggccgtc ttcacgacgt cgaggttgtg ctcgatgacg atcaccgtgt tgccgcggtc
     1261 gacgagggcg tggaggacgt cgaggagctt cctcacgtcg tcgaagtgca ggccggtcgt
     1321 cggctcgtcg aggatgtaga gggtccgccc cgtagcccgc cgcgccagct cgcgggcgag
     1381 cttcacccgc tgcgcctcgc cccccgagag cgtcgtcgcc ggctggccga gcttgatgta
     1441 gccgagcccg acctccacga gcgtcgcggc gacgttgcgg atcgtcggca cgttcgcgaa
     1501 gacctcggcc gcctgctcgg ccgtcaggtc gagcacctcg gccacgttca gccccttgta
     1561 gcgcacctcg agcgtctcgc ggttgtaccg ccggccgccg cagacgtcgc acgtcacgta
     1621 gacgtcgggg aggaagtgca tctcgatggc ggtctgcccg ccgccgccgc acgcttcgca
     1681 gcgccccccc ttcacgttga acgagaagcg ccccggcccg tagccgcgca tccgcgcgtc
     1741 cggcacctgc gccatcaggt cgcggatcgg cccgaagagg ttcgtgtagg tcgccgggtt
     1801 cgagcggggc gtccgcccga tcggcgactg gtcgatgtcg atcaccttgt cgacgtgctc
     1861 gagcccctcg atccgctcgt gctcccccgg cttctcgtgc gcgccgtgga ggaggcgcgc
     1921 cgcggcgcgg aacaggatgt cgttcacgag cgtcgacttc cccgaccccg agacgcccgt
     1981 caccgccgtc aggcagccga gcgggaagcg ggccgtcacg tcccgcaggt tgttcgcccg
     2041 cgcgccgacg accgtgatcg ccttcccgct cccggcgcgg cgctcctccg gcaccgggat
     2101 cgtcagctcg cgcgagaggt acttccccgt cagcgagcgc gggaagcggc ccagctcgtc
     2161 cgccgcccct tcgcccacca cctcgccgcc gtggacgccc gcccccgggc cgaggtccac
     2221 gacccggtcc gcggcccgga tcgtctcctc gtcgtgctcc acgacgacga cggtgttccc
     2281 gaggtcgcgc atcgccatca gcgtctcgat cagcttccgg ttgtccttcg ggtggaggcc
     2341 gatcgagggc tcgtcgagga cgtagagcac gcccatcagc ttcgagccga tctgcgtcgc
     2401 gaggcggatc cgctgcgcct cgcctcccga gagcgtcgcc gacgcgcggc cgagcgagag
     2461 atagccgatc ccgacgtcgt tcaggaagcc gagccggtcc tcgatctcct tcaggatctt
     2521 cccggcgatc tcacgctccc gcggctccgg cccgaagctc gcgaagaagg ccttcgcgtc
     2581 ctcgagcgtc atctccgcga gcgcgtcgat cgcctgcccg cgcaccttca ccgcgagcgc
     2641 ctcggccttc agccgccgcc cgccgcactc cgagcacggc gtgagcgaca tgaacgcctc
     2701 cagctcctgc cgcgcctcct cgctctcgac ctccttgtac ttctccgtca ggagcgggac
     2761 gagccccttg taggaggacg accagacgta ctcccccttc tccgacttcc acttccactt
     2821 gatctcctgc tccttcgagc cgtggaggat catgtcccgc acgccggccg ggagcttctt
     2881 ccaggggacg tccatcgaga acttcgccgc cttcgagagc tgctcgagct gccggaggcg
     2941 ccagttcgac gaccccttct tccagagcgc gatcgcgccg tcggcgatgg agagcgtcgg
     3001 gtcggggacg aggcggtcgg ggtcgacgcg cggcaccgag ccgagccccg agcaggccgc
     3061 gcacgccccg tacggcgtgt tgaacgagaa aaggcgcggc gtgatctccg ggagcgacgt
     3121 cccgcagtcg gggcaggcgt acgacgtgga gagcgtctcc tccgagagcg tctccccctt
     3181 gccgcccacg accgagatcg tcacgaggcc gtccgccacg cgcaccgccg tctcgagcga
     3241 gtcggtcaga cggcggtgcg cctcgtcgtc ccgccggacc accaggcggt cgacgatcac
     3301 ctcgatcgtg tgcttcttct gcttgtcgag ctc
//
LOCUS       DAOTEZ010000464         3318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_22004_length_3318_cov_4.390745, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000464 DAOTEZ010000000
VERSION     DAOTEZ010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22004_length_3318_cov_4.390745"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="P5164_17855"
     CDS             complement(<1..560)
                     /locus_tag="P5164_17855"
                     /inference="COORDINATES: protein motif:HMM:NF027583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HRY45800.1"
                     /translation="MTEPTAPDPAPAPETEPATPAEAPPVDGDRAEIEEIPGYDGHAK
                     VDRPSQDPIAPPPEDPDVPETPFEKVLRELPYLPTRYDETYVYLVPCDPKSLYVIWEV
                     ATEAREALKARFGADFFEKNALLLRVYDVTGVSFDGFNASSFFEVDDYLADKSEYWVA
                     VAGGRDYVAELGYRALGTTYFEKVARS"
     gene            559..921
                     /locus_tag="P5164_17860"
     CDS             559..921
                     /locus_tag="P5164_17860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45801.1"
                     /translation="MAWVASFFLVGFLAGSLRAGFGSGFAAGLEAGALRASAAGFAAS
                     ALRAGAFRLTRRIGAVSFGAFAPGVTAAAGSACRAGTASGALFGVLGPAFVFRGPGPS
                     FSFFRFDFSSMVLVPFGS"
     gene            complement(1042..2163)
                     /locus_tag="P5164_17865"
     CDS             complement(1042..2163)
                     /locus_tag="P5164_17865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY45802.1"
                     /translation="MRIETLAVHAAYSVDPASGAVAPPIHLSTTFGRDEAGAPLAGHT
                     YIRESSPTQDLLEEALARLEGGEAALVFGSGMAAGLAVVQALPPGSHVVLPDDAYYGF
                     RVAARDFFPQWGISATFSPLDRTGALAAALRPETRLVWLESPSNPLLKVTDLERAASI
                     AKRAGALVLVDGTFATPILQRPIELGADVVLHATTKGMGGHSDVAGGALVFAARGELL
                     EKVRHVRTVLGAVSSPFSSWLVLRGLRTLAVRARVQSETALAVACALEAHPGVSRVNY
                     PGLASHPGHEVARRQMSAFGSMISFHVAAGREAAIAAVGRARLFTRATSLGGVESLIE
                     HRATSEGDASTAPPDLVRLSIGLEHADDLVADLDAALRA"
     gene            complement(2193..>3318)
                     /locus_tag="P5164_17870"
     CDS             complement(2193..>3318)
                     /locus_tag="P5164_17870"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY45803.1"
                     /translation="MQRVAQGWLVLTLTGSPFWLGVDAFLGEAPFLAFSLFGGVLADR
                     AERRRILLASQLVQMASALVLMTLIATGRITLGAILAVSVVVGLAQAFGGPAFQALVP
                     MLVPPADLPRAILLNSIQFNFARVVGPAVGGLAFARFGAAGCMGLNGLSFLAVVAALA
                     AIPRRPGTGSDGATVLEGLREGIGTVWRRPSLRGLVGLAFLGTFCSNPLLTFLPVFAR
                     DVFHAGAERYSVLLAAFGCGAVFGGLAVAGAARLPRRGLLSALGLVLYGVLMVAFALN
                     REPILSLVLLVAAGACQMVVFSGFMTLVQTSVGDALRGRVVSIYSLAFRGGMPLGNLS
                     AGTAAAAVGAPAVLVVLGGALAIAGGIVAHRRGRDGVASI"
BASE COUNT          475 a         1186 c         1239 g          418 t
ORIGIN      
        1 gagcgggcga ccttctcgaa gtaggtcgtc ccgagggccc ggtagccgag ctcggcgacg
       61 tagtctcgcc cccccgccac ggccacccag tactcggact tgtcggcgag atagtcgtcc
      121 acctcgaaga aggagctcgc gttgaacccg tcgaaggaga cgccggtgac gtcgtagaca
      181 cggaggagaa gggcgttctt ctcgaagaag tcggcgccga atcgcgcctt gagcgcctcg
      241 cgggcctccg tcgcgacctc ccagatcacg tagaggctct tcgggtcgca cggcacgaga
      301 tagacgtagg tctcgtcgta cctcgtcggc aggtacggca gctcgcgcag gaccttctcg
      361 aacggagtct cggggacgtc cgggtcctcg gggggaggcg caatcgggtc ctgcgacggg
      421 cggtcgacct tcgcgtgccc gtcgtagccg gggatctcct cgatctcggc gcgatcgcca
      481 tccacgggcg gcgcttcggc gggcgtcgcc ggttcggtct cgggagcggg agcaggatcc
      541 ggggcggtcg gctcggtcat ggcctgggtc gcctccttct tcctcgtggg cttcctcgcg
      601 gggtccttgc gggccggctt cggttccggc ttcgcggccg gcctcgaagc cggggccttg
      661 cgagcctccg cggccggctt cgcggcgtcc gccttgcgcg ccggggcctt ccggcttacc
      721 cgtcggatcg gggccgtctc cttcggagcc ttcgccccgg gggtcacggc ggcggcgggc
      781 tcggcctgcc gggccggcac ggcttcgggg gcgctcttcg gcgtcctcgg tcccgctttc
      841 gtcttccggg gcccggggcc gtccttctcg ttcttccgct tcgacttctc gtccatggtc
      901 ctcgttccgt tcggatcgtg atcccggccc cgcgaggaca caacgtcccg ccttcgggcc
      961 agaggccggt tcgtggctcc cggtctcggg tccttgtccg gcggcgtccc gatcatacgg
     1021 gatcgaccgt gaagaccgcg ttcaggcgcg gagcgccgcg tcgaggtcgg ccacgaggtc
     1081 gtccgcgtgc tcgaggccga tcgagagccg gacgaggtcc ggcggtgccg tcgaggcgtc
     1141 cccctcgctt gtcgcccggt gctcgatgag gctctcgact ccgccgagcg aggtcgcgcg
     1201 ggtgaagagc cgcgcccgcc ccaccgcggc gatcgccgcc tcgcgccccg cggcgacgtg
     1261 gaacgagatc atcgagccga aggcgctcat ctgccggcgc gcgacctcgt ggccgggatg
     1321 ggaggccagt cccgggtagt tcaccctcga gacgccgggg tgcgcctcga gcgcgcaggc
     1381 caccgcaagg gccgtctccg actgaacccg cgcccggacc gcgagcgtcc gcagtccccg
     1441 gaggacgagc caggagctga acggcgagga gaccgccccg aggaccgtcc gcacgtgccg
     1501 caccttctcg aggagctcgc cccgggccgc gaagacgagc gcgccccccg cgacgtcgct
     1561 gtgcccgccc atccctttcg tcgtcgcgtg gaggaccacg tcggccccga gctcgatcgg
     1621 ccgctggagg atcggcgtgg caaaggtgcc gtcgacgagg acgagcgctc ccgcccgctt
     1681 cgcgatcgag gctgccctct cgaggtccgt gaccttcagg agcgggttcg aagggctctc
     1741 gagccagacg agacgggtct ccgggcggag cgccgccgcg agggcgcccg tccggtcgag
     1801 cggtgagaac gtcgccgaga tcccccactg cgggaagaag tcgcgcgcgg cgacccgaaa
     1861 gccgtagtag gcgtcgtccg ggaggacgac gtggcttccg ggcgggaggg cctggacgac
     1921 ggcgagcccg gccgccattc ccgagccgaa gacgagcgcc gcctcgcccc cttccaggcg
     1981 tgcgagcgcc tcctcgagca ggtcctgcgt cgggctcgac tcccggatgt aggtgtggcc
     2041 ggcgagcgga gcgcccgcct cgtccctccc gaacgtcgtc gagaggtgga tcggcggcgc
     2101 gaccgcgccg gaggcggggt cgacggagta cgcggcgtgg acggcgagcg tctcgatcct
     2161 catgcgccgc agcctacagg agccgccgcc cctcagatcg aggcgacgcc gtcccgtccc
     2221 cgccggtgcg cgacgatccc gcccgcgatc gcgagcgccc cgccgaggac gacgaggacg
     2281 gcaggcgcac cgaccgccgc cgcggccgtc cccgccgaga ggttcccgag cggcatgccg
     2341 ccccggaagg cgaggctgta gatgctgacg acccggcccc ggagcgcgtc gccgacgctc
     2401 gtctggacga gcgtcatgaa cccggagaag accaccatct ggcacgcccc ggcggcgacg
     2461 aggaggacga gagagaggat cggctcgcgg ttgagcgcga aggcgaccat gaggacgccg
     2521 tagaggacga gcccgagggc tgagagcagg ccccgccgcg gcagccgggc cgcacccgcg
     2581 accgcgagcc cgccgaagac cgccccgcag ccgaacgccg cgaggaggac gctgtagcgc
     2641 tccgctccgg cgtggaagac gtcccgggcg aagaccggga ggaacgtcag gagggggttc
     2701 gagcagaacg tgccgaggaa ggcgaggccg acgagacccc gcagcgaggg ccgccgccag
     2761 acggtcccga tcccctcgcg aaggccctcc aggaccgtcg ccccgtcact gcccgttccg
     2821 gggcggcggg ggatcgcggc gagcgccgcc acgaccgcga ggaaggagag accgttgagc
     2881 cccatgcagc ccgcggcgcc gaagcgtgcg aaggcgaggc cgccgaccgc cgggccgacg
     2941 acgcgcgcga agttgaactg gatggagttc aggaggatcg cccgcggcag gtcggccgga
     3001 gggacgagca tcggaacgag ggcctggaac gcgggcccgc cgaacgcctg ggcgagcccg
     3061 acgacgaccg agaccgccag gatcgccccg agcgtgatcc gcccggtggc gatcagcgtc
     3121 atcaggacga gcgcggaggc catctggacg agctgcgagg cgaggaggat ccgccgccgc
     3181 tccgctcggt cggcgaggac tccgccgaag agcgagaagg cgaggaacgg ggcctcgccg
     3241 aggaaggcgt cgacacccag ccagaagggc gagccggtca gggtcaggac gagccacccc
     3301 tgggcgaccc gctgcatc
//
LOCUS       DAOTEZ010000465         3309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_22091_length_3309_cov_6.598955, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000465 DAOTEZ010000000
VERSION     DAOTEZ010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22091_length_3309_cov_6.598955"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..715)
                     /locus_tag="P5164_17875"
     CDS             complement(38..715)
                     /locus_tag="P5164_17875"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRY45804.1"
                     /translation="MTRSRPLLLLVVASASLLPACTPKNALRMAIEVVSEPDAAIVSF
                     KGKDVGTTPASLRVKTFDELMTIAARKDDAPVAEKRIRILSPDHAQLVFRFGAEPSEV
                     AKRLGLARVVVFDYSEKVSFDVSRSELKPEGLPILDRQAEILARYFPDVDVYVCGHTD
                     ATGGDDFNLQLSVDRARVVADRLSGKGVPPGRLKVQGFGKEFPNDTNETPAGRANNRR
                     TELILPQ"
     gene            complement(765..2369)
                     /locus_tag="P5164_17880"
     CDS             complement(765..2369)
                     /locus_tag="P5164_17880"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRY45805.1"
                     /translation="MERDLESLRALLADRYRIDGELGRGGYAVVYRAWNLRLERAEAL
                     KVLSPKRGDDDDFAPRFMQEVRLAAGLEHPSIVKVYDYGESGGTIWYSMQLVDGQSLA
                     VRLRRAGAFSEPEAARLAVPLLDALAYSHERGIVHRDIKPENVLLDADGRPYLTDFGI
                     AKSAGSLVKTQTGFLMGTPAYVSPEQAQAKPLDGRTDLYALGVTLYRIVSGRYPFEAP
                     DPLGAVVLRLTQPPRPLVEVLPSVEPGFAEIVMRALERDPALRWGSARAMGDAFAGFL
                     AGKPAGSGRLRVPKASATPQSAEVATAPDEPSEATRLGPAGQVGVPISPSATVPPAER
                     PGRRPRRLGVLGLAGAVVGLIVLLGIVTLLVAPRRDRETDPSRTPPSPHSTPSAPTSA
                     APTPVVADRPITEASSKERATSTAAPNTESAPAAPSRRRAPAETLPEPPPIRRPVNTP
                     ERLDPEPPFAGPPPAGCPGARVRVGFAVDETGKITAPRLFPSEAPPECGRFVLDALPG
                     WRWRPAVDASGAPVPSARLIVEIQLP"
     gene            complement(2392..3078)
                     /locus_tag="P5164_17885"
     CDS             complement(2392..3078)
                     /locus_tag="P5164_17885"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HRY45806.1"
                     /translation="MSKPIPEGLWELLPLLFRPHPWHGISVGEEAPDKVTAYIEIVPT
                     DPVKYELDKTTGYLKIDRPQKFSNVYPSLYGFIPQTFCGEENAAFCGQRTGRDGIVGD
                     GDPLDVCVLSEKDISHGDVLMNVVPIGGLRMIDNHEADDKIVAVLEGDAVFGQLREIY
                     QLPPTLVDRLKHYFLTYKQAPDSLQPHRVEITHVYGREEAHEVIRRSRADYDKRYGKL
                     RELLTAALRA"
BASE COUNT          475 a         1093 c         1269 g          472 t
ORIGIN      
        1 ggagggagcg acggaagagc cgtccgtccc ggctcctcta ctgcggcagg atcagctccg
       61 tccgcctgtt gttcgcgcgg cccgcggggg tctcgttcgt gtcgttcggg aactccttcc
      121 cgaaaccctg gaccttcagg cgcccgggcg ggactccctt gccggagagg cggtcggcga
      181 cgacccgcgc gcggtcgacg gagagctgca ggttgaagtc gtcgccgccg gtcgcgtcgg
      241 tgtggccaca gacgtacacg tcgacgtccg ggaagtagcg ggcgaggatc tcggcctggc
      301 gatcgaggat cggaagcccc tccggcttca gctcgctcct gctgacgtcg aaggagacct
      361 tctccgagta gtcgaacacg acgacgcgag ccagtccgag gcgcttcgcc acctccgacg
      421 gctccgcgcc gaagcggaag acgagctggg cgtggtcggg cgagaggatg cggatccgct
      481 tctcggcgac cggcgcgtcg tcctttcggg ctgcgatcgt catcagctcg tcgaacgtct
      541 tcacgcggag ggaggcaggt gtcgtcccga cgtccttccc cttgaaagag acgatcgccg
      601 cgtccggctc cgagacgacc tcgatcgcca tccgaagggc gttctttggg gtgcaggccg
      661 ggaggagcga agccgaggcg acgacgagga gaaggagggg ccgggaccgt gtcatctggg
      721 cgtctccctt cggatcgaga tcagccgatg atagtcgcgc aagctcaggg cagctggatt
      781 tcgacgatga gccgggccga cgggaccggg gcgccggagg cgtccaccgc gggccgccac
      841 ctccagcccg ggagcgcgtc gaggacgaaa cggccgcact ccggcggcgc ctccgaggga
      901 aagagccgcg gggcggtgat cttccccgtc tcgtccacag caaagccgac cctcaccctt
      961 gcccccgggc agcctgccgg gggcggcccg gcgaaggggg gctccgggtc gaggcgttcg
     1021 ggggtgttga cggggcggcg gatcgggggc ggttccggaa gggtctcggc tggcgcccgc
     1081 cgtcgggaag gtgcggcggg cgccgattcg gtgttcggcg ccgccgtcga cgtcgcacgc
     1141 tccttcgaag aagcctcggt gatcggccgg tcggcgacga cgggcgtcgg agccgccgag
     1201 gtcggagcgg acggggtcga gtgcggcgaa gggggcgttc gggaggggtc cgtctcgcga
     1261 tcgcggcggg gggcgacgag gagcgtcacg atcccgagga ggacgatgag accgaccacg
     1321 gcgccggcga gcccgaggac gccgagcctg cgtggccgtc gtccgggcct ctcggcgggg
     1381 ggcaccgtcg ccgaaggcga gatcgggacg ccgacctgtc cggccgggcc gagccgggtc
     1441 gcctcggaag gttcgtcggg cgccgtggca acctccgcgg actgcggcgt cgcggaggcc
     1501 ttcgggacgc ggagccgccc gctccccgcc ggcttcccgg cgaggaagcc ggcgaacgca
     1561 tcccccatcg cgcgagcgga cccccatcga agggccgggt cccgctcgag ggcccgcatg
     1621 acgatctccg cgaacccggg ttcgaccgaa gggaggacct cgacgagggg acgaggcggc
     1681 tgggtgaggc ggaggacgac cgccccgagc gggtcgggcg cctcgaacgg gtagcggccc
     1741 gagacgatcc ggtagagcgt gacgccgagg gcatagaggt cggttcggcc gtcgagcggc
     1801 ttcgcctggg cctgctcggg ggagacgtag gcgggtgtcc ccatcaggaa gccggtctgg
     1861 gtcttgacga gcgacccggc cgatttcgcg atgccgaaat ctgtgaggta cggccggccg
     1921 tccgcgtcga ggaggacgtt ctcgggcttg atgtcacggt ggacgatgcc gcgctcgtgg
     1981 ctgtaggcga gggcgtcgag gagcgggacg gcgaggcgcg ccgcctccgg ctcggagaag
     2041 gcgcccgcgc ggcgaagccg cacggcgagc gactgcccgt cgacgagctg catcgagtac
     2101 cagatcgtcc cgcccgattc gccgtagtcg tagaccttca cgatcgacgg gtgctcgagg
     2161 cccgccgcga ggcgcacctc ctgcatgaag cgcggggcga agtcgtcgtc gtcccccctc
     2221 ttcggcgaga ggaccttcag ggcctccgcg cgctcgagcc gcaggttcca ggcccggtag
     2281 acgaccgcgt atcccccccg cccgagctcg ccgtcgatcc gatagcgatc ggcgaggagc
     2341 gcgcgaaggc tctcgaggtc tctctccatc tggaggcgcg acgggggtgc gtcaggcccg
     2401 aagcgcggcg gtcaggagct cgcggagctt cccgtatctc ttgtcgtagt cggcccggct
     2461 gcggcggatc acctcgtgcg cctcctcgcg gccgtagacg tgcgtgatct ccacgcggtg
     2521 cggctggagg gagtcgggcg cctgcttgta ggtgaggaag tagtgcttca gccggtcgac
     2581 gagcgtcggc gggagctggt agatctcccg cagctgtccg aagacggcgt ccccctcgag
     2641 gacggcgacg atcttgtcgt ccgcctcgtg gttgtcgatc atccggaggc ccccgatcgg
     2701 gacgacgttc atcaggacgt cgccgtggga gatgtccttc tcggagagga cgcagacgtc
     2761 gagcgggtcg ccgtcgccga cgatcccgtc ccggcccgtc cgctgcccgc agaaggcggc
     2821 gttctcctcg ccgcagaacg tctgcgggat gaagccgtag agcgacgggt agacgttcga
     2881 gaacttctgc ggccggtcga tcttcaggta gccggtcgtc ttgtcgagct cgtacttgac
     2941 cgggtcggtc gggacgatct cgatgtaggc ggtcaccttg tcgggggctt cttcgcccac
     3001 cgagatcccg tgccaggggt ggggccggaa gaggagcggg aggagctccc agaggccctc
     3061 ggggatcggc ttgctcatgc ggcaacgcta ccagaatcgc cgggaggcgg acggccggag
     3121 gggcccgggc cgcgacgcgc cccggcgcga gatactccgc cccgatgccg cccgaggccc
     3181 cgccccccgc cgcgaagcgc tacgtcctcc ggaaggcggg gccggcgaag agctatcgga
     3241 tcgactaccg gcgggagctg aacgccgagc agcaggcggt cgtcttcgcc cccgacggcc
     3301 cgaccgtcg
//
LOCUS       DAOTEZ010000466         3306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_22110_length_3306_cov_5.659182, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000466 DAOTEZ010000000
VERSION     DAOTEZ010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22110_length_3306_cov_5.659182"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="P5164_17890"
     CDS             <1..553
                     /locus_tag="P5164_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019641775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HRY45807.1"
                     /translation="LSLDGPVERLRARASFLRRAMPEVDLPPLDDEVLRSLLAELVVG
                     RRRLSELRAVPLEPLLRARLGSRALRLLDLHAPAALPVPTGRDVPLEYGADGPPVLAV
                     KLQELFGLSESPRVADGRVPVLLHLLSPAGRPVQVTSDLASFWSRTYPEVRKELRGRY
                     PKHPWPEDPRGAPPRRGTTRSGR"
     gene            584..1150
                     /locus_tag="P5164_17895"
     CDS             584..1150
                     /locus_tag="P5164_17895"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HRY45808.1"
                     /translation="MKVLRVLLAPLLALPLTAAEPPAPEALASQARGLAGNLMLSLQG
                     ELFAAMKSGGPVKALEVCRSRAPEIAAATAMGTPWKIARTSQRVRNGANAPDEWEARK
                     LEELATRLSAGEELSALEAWEVVEVDGRPAFRYMKAIGTASPCLTCHGSALKPEIASK
                     VRELYPDDRAVGFAAGDLRGAFTLSRPL"
     gene            1188..2702
                     /locus_tag="P5164_17900"
     CDS             1188..2702
                     /locus_tag="P5164_17900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HRY45809.1"
                     /translation="MTTRIRNRALLDRRVSAEEAAAMIRPGDTVGMSGFTGAGYPKAI
                     PVALAKRIMDASLRGEKFRVGIWTGASTAPELDGALAMVDGVELRLPYQSDPTCRKKI
                     NAGDMEYLDIHLSHVGAFVRQGFLGKLDVALVEVTGIREDGALIPASSIGNNKVWLDS
                     AERVILEVNSWQSESLEGVHDVYQGFALPPNRKPIPLVGPADRIGTPYLQCPLEKIVA
                     IVETNAPDRNAPFSAPDEASKRIAGHILEFLGDEVKKGRLPKNLLPLQSGVGNIANAV
                     LFGLEDGPFTDLTAYTEVLQDGMLSLIASGKLRVASATAFSLSPQKVEELNSQMDRYR
                     DSLILRPQEISNHPELIRRLGCLAMNGMIEADIYGNVNSTHVNGTAIMNGIGGSGDFA
                     RNAYISFFMTPSTAKKGAVSCIVPMVTHVDHTEHDVHVLVTEQGLADLRGLSPKQRAE
                     RVIEKCAHPDYRPALRDYVRRAGTASFGRHTPHLLDEAFAWHRHLLRTGTMKGA"
BASE COUNT          497 a         1263 c         1108 g          438 t
ORIGIN      
        1 cctctcgctc gacggacccg tggagcgtct tcgcgcccgc gcctccttcc tccggagggc
       61 gatgcccgag gtggacctgc ctcctctcga cgacgaggtc ctgcggtcgc tcctcgccga
      121 gctcgtcgtc ggacgccggc gcctctccga gctgcgcgca gttcctctcg aaccgctcct
      181 ccgcgcccgg ctcgggtccc gcgcgctccg cctcctcgac ctccacgccc cggccgcgct
      241 cccggttccg accggccggg acgtcccgct cgagtacggc gccgacgggc cgccggtcct
      301 cgcggtgaag ctccaggagc tcttcggcct ctccgagtcg ccgcgcgtgg cggacgggcg
      361 cgtcccggtc ctcctccacc tcctctcccc ggcgggacgc ccggtacagg tcacctccga
      421 cctcgcgagc ttctggagcc gcacgtaccc cgaggtgagg aaggagctga ggggacgcta
      481 cccgaagcac ccctggccgg aggacccgcg cggcgccccg ccgcgccgcg gcacgacccg
      541 ctcgggccgc tgacgcgcta ccctcccttc gacggaggtc gagatgaagg tcctgagagt
      601 tctcctcgcc cctctcctcg cgcttcccct caccgcggcc gagcccccgg ccccggaggc
      661 gctcgcctcg caggcgcgcg gcctcgccgg gaacctgatg ctctcgctcc agggcgagct
      721 cttcgccgcg atgaagtccg gcggccccgt gaaggccctc gaggtctgcc gaagccgcgc
      781 gcccgagatc gccgcggcga ccgcgatggg gaccccctgg aagatcgcgc gtacgtcgca
      841 gagggtccgc aacggcgcga acgcgccgga cgagtgggag gcgcggaagc tcgaggagct
      901 cgcgacgcgg ctctccgcgg gcgaagagct ctccgccctc gaggcctggg aagtcgtcga
      961 ggtcgacggg cgtccggcgt tccgctacat gaaggcgatc ggcaccgcct cgccctgcct
     1021 cacttgccac ggctcggcgc tcaagcccga gatcgcgtcg aaggtgcgcg agctctaccc
     1081 ggacgaccgg gccgtcggct ttgcggcggg agacctccgc ggcgccttca ccctctcccg
     1141 gcctctctga tcccggccgc cgcccggctc gaaggaggat ccccgccatg acgacgcgca
     1201 tccggaaccg cgccctgctc gacaggcgag tctcggccga agaggccgcg gcgatgatcc
     1261 gacccggcga caccgtcggg atgagcggct tcaccggggc cggctacccc aaggccatcc
     1321 cggtcgccct ggcgaagcgg atcatggacg cgagcctccg cggcgagaag ttccgcgtcg
     1381 ggatctggac gggcgcgtcg accgcgcccg agctcgacgg cgccctcgcg atggtcgacg
     1441 gcgtggagct gcggctcccc taccagtccg acccgacctg ccggaagaag atcaacgcgg
     1501 gcgacatgga gtacctcgac atccacctct cgcacgtcgg ggcgttcgtg aggcagggct
     1561 tcctcggcaa gctcgacgtg gcgctcgtcg aagtgaccgg catccgcgaa gacggcgcgc
     1621 tcatccccgc ctcctcgatc gggaacaaca aggtctggct cgactcggcc gagcgggtca
     1681 tcctggaggt caactcctgg cagtcggaga gcctcgaagg ggtgcacgac gtctaccagg
     1741 ggttcgcgct cccgccgaac cgcaagccga tcccgctcgt cggcccggcg gatcggatcg
     1801 gcacgccgta cctccagtgc ccgctcgaga agatcgtcgc gatcgtcgag acgaacgcgc
     1861 ccgaccgcaa cgcgcccttc tcggcgcccg acgaggcgtc gaagcggatc gcggggcaca
     1921 tcctggagtt cctcggcgac gaggtgaaga aggggcgtct cccgaagaac ctcctcccgc
     1981 tccagtcagg cgtcggcaac atcgcgaacg cggtcctctt cggcctcgag gacggcccgt
     2041 tcaccgacct gacggcctat accgaggtgc tccaggacgg gatgctctcg ctcatcgcct
     2101 ccgggaagct gcgcgttgct tccgcgaccg ccttctccct cagcccgcag aaggtcgagg
     2161 agctgaactc ccagatggac cgctaccgcg acagcctgat cctccgcccc caggagatct
     2221 cgaaccaccc cgagctgatc cgccgcctcg gctgcctcgc gatgaacggg atgatcgagg
     2281 ccgacatcta cgggaacgtc aactcgacgc acgtgaacgg caccgcgatc atgaacggca
     2341 tcggcggctc gggcgacttc gcgcgcaacg cctacatctc cttcttcatg acgccctcca
     2401 cggccaagaa gggcgccgtc tcctgcatcg tcccgatggt cacgcacgtc gaccacacgg
     2461 agcacgacgt ccacgtcctc gtcacggagc aggggctcgc cgacctgcgg ggcctctcgc
     2521 cgaagcagcg cgccgagcgc gtcatcgaga agtgcgccca ccccgactac cgccccgccc
     2581 ttcgcgacta cgtgagacgc gcgggcaccg cctccttcgg ccgccacacg ccccacctcc
     2641 tcgacgaagc cttcgcctgg caccgccacc tcctccgcac cggcacgatg aagggagcct
     2701 gaggccgccg gggcaccaga gcacgggaga acgggaacac gagagcacga gagcacgaga
     2761 gcacgagagc acgagagcac gagagcacga gagcacggga gcacgagagc acgggagcac
     2821 gagagcaccg gggcacgagg gagggagcct cgccgcggga tgggtcccac ggccgctggc
     2881 tgcgcgcggc cgacccgctg acgcgcccgc tgcggccggg cggggcgccc gcgcaagctc
     2941 gcttcgctcg aatcggtgcg cgggcttctt ccccgcccgg tcctcgcgat cgcgagcggg
     3001 tccccggccg tgctcgaggc ggctcgcggg acccatcccg cggctcggcc caccggagca
     3061 ggaccaagga gcgcggatcg gctctcgttc cgaggtctgt cttcgtaggg caggccgact
     3121 gccattcagg cccgatcgcg aagcgccgag tcttgccctc gctggttctc gctgacctgc
     3181 gcgtggcgtg agcggacggg aggggcgtcc cgcgagcgag cgttcgagca cgcgcgggga
     3241 ccccgcacgg gtccgcgagc aggccatcgg gagaagcgga cgcgctgttt gacgagcgaa
     3301 gcgagg
//
LOCUS       DAOTEZ010000467         3301 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_22173_length_3301_cov_5.349661, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000467 DAOTEZ010000000
VERSION     DAOTEZ010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22173_length_3301_cov_5.349661"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2778
                     /locus_tag="P5164_17905"
     CDS             <1..2778
                     /locus_tag="P5164_17905"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRY45810.1"
                     /translation="IIGQVAEALDYAHRRGIVHRDVKPANIIITPEGQVKITDFGIAK
                     IEKSNLTSTGQFLGTPNYMSPEQVTGDAVDGRSDLFSLGVVLYELLTRKKPFTAENLT
                     SISYKIVHERFVPPETYDAGIPTEFVGVLDKALAKDPAERFQRGNDFALALFEFKARE
                     EERQMLRDLGQMVAEAEKLGPVATVTAPPSTLPTPAPAAAPAMPAPPAMGRSPFEILA
                     AAPVVPAPPAAPSLRAPDDDLATREVHRPLPATAYVAGPAPASAGGGEGARSGSSEEW
                     QLDEPTRSKRKGAPPPPPPVAGDVDVEASLPGAEVVEAAVPAPVPPSPAASGLAVSPF
                     DDAPAERRPDGATEILRIDAIPGLPAPVADRPTEILKPIVPPTPPAPAAPPVPSAAPP
                     PVADRPTEILRVVPPPPRVGAPATPTDQPTEILRPIAPSPAPATPPAPVMAPPASAPS
                     TPAPFADRPTEILKPIAPPAAAAPPAVTAPPAPAAPSIPAAATAASAPAPAVSDRPTE
                     ILRSPFPSPAPVARPPAPQAPGDAAGEEGATERIVDAMKLARSGAPSTPAAPKAPPSP
                     KTPPSSKTPPPVATGAPAASKAVPAPSPAALPPLEPAPIAESRPAPPAPPAEPLMKRS
                     VNLRFVALIIALVAVAGAVVTGVLWSQKKKVEAQAAVDELREKEVREQKALMDEGTRL
                     LDAGQPSAALEKFRELVRRKPDSEAVRTAIARAEELVRAQQSGEELSREVETRMATAR
                     EATESGEFDRALDQLAAVLLLQPENAEALALRESVRTAQAEQTAAQKKAAAEAARRKR
                     PTPVPTATPLPRVALPAEAPRAATPTPGVARLRIVFQSPAPEGYVMIRRNDKEVFRRN
                     FDFGRKSAGGLVEGTIEVPSGGASYKAWAIPTNRSFNGYKAVDLVVPGGETQTLLLEL
                     NAEKELVVRLR"
     gene            2744..>3301
                     /locus_tag="P5164_17910"
     CDS             2744..>3301
                     /locus_tag="P5164_17910"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRY45811.1"
                     /translation="MRKKSSSSGSVRPLLLALASFPLFVAVARGDERASFGGAGHQAP
                     APVLTSSRGVVVSAAPEASQAGAEVLAAGGNAMDAALAAAFALAVVYPQAGNLAGGGF
                     LVVRTPDGVVRALDFRETAPAGATREMFLGPDGRPVTGSSTETMLAVATPGSVRGYAE
                     AHRLLGRLPWAKVVAPAERLAREGFV"
BASE COUNT          442 a         1281 c         1165 g          413 t
ORIGIN      
        1 atcatcggcc aggtcgccga ggcgctcgac tacgcccacc gccgcgggat cgtccaccgc
       61 gacgtcaagc cggcgaacat catcatcacg cccgaggggc aggtgaagat caccgacttc
      121 gggatcgcga agatcgagaa gtcgaacctc acctccaccg ggcagttcct cggcacgccg
      181 aactacatgt cgcccgagca ggtgacggga gacgccgtcg acggccggag cgacctcttc
      241 tccctcggcg tcgtcctcta cgagctcctg acgcggaaga agccgttcac cgcggagaac
      301 ctgacgtcga tctcgtacaa aatcgtccat gagcggttcg tcccgcccga gacctacgac
      361 gccgggatcc ccaccgagtt cgtcggagtc ctcgacaagg cgctcgccaa agacccggcg
      421 gagcgattcc agcgggggaa cgacttcgcc ctcgccctct tcgagttcaa ggcccgggaa
      481 gaggagcgcc agatgctccg cgatctcggg cagatggtcg cggaggcgga gaagctcggc
      541 cccgtcgcga cggtgacggc acctccgtcg acgctgccga cgccggctcc ggccgcggcg
      601 ccggcgatgc ccgcgccccc ggcgatgggg cgttcgcctt tcgagatact ggcggcggcg
      661 cccgtggtcc ctgctccgcc cgcagccccg agtctgcggg cgccggacga cgacctcgcc
      721 acccgcgagg tccacagacc tcttcccgcg acggcctacg tggccggccc ggctccggcg
      781 agcgccggcg ggggcgaggg cgcgcgttcg ggaagctcgg aggagtggca gctcgacgag
      841 ccgacgcgct cgaagaggaa gggagctcct cctccgcccc cgccggtcgc gggcgacgtc
      901 gacgtcgagg cttcgctccc cggcgccgag gtcgtggagg cggccgtgcc ggcacctgtg
      961 ccgccgtctc cggcggcgag cggcctcgcg gtttctccgt tcgacgacgc gccggcagaa
     1021 cgacggccgg acggcgcgac ggagatcctc cggatcgacg cgatcccggg gcttcccgcg
     1081 cccgtcgccg atcgcccgac ggagatcctg aagccgatcg tcccgccgac accgcctgct
     1141 ccggcggcgc caccggttcc gtcggcggcc cctcccccgg tcgcggaccg tccgacggag
     1201 attctgcggg tcgtaccgcc gccgccgcgc gtcggggctc cggcgacgcc gaccgatcag
     1261 ccgacggaga tcctcaggcc gatcgctcct tcgccggccc cggcgacgcc gccggcgccc
     1321 gtgatggcgc cgccggcctc cgcgccctcg acgcccgcgc ccttcgccga tcgtccgacg
     1381 gagatcctca agccgatcgc gccgccggcc gcggcggcac cgccggccgt gacggcccct
     1441 cctgcgccgg ccgccccctc gattccggcc gcggcgacgg cggcttccgc ccctgcgccg
     1501 gcggtctcgg atcgcccgac ggagatcctc cgcagcccgt tcccgagccc tgcgcccgtc
     1561 gcgcggcccc cggcaccaca ggctcccgga gacgcggccg gcgaagaggg cgcgaccgag
     1621 cggatcgtcg acgccatgaa gctcgcgcgc tccggggcgc cttcgacacc ggctgctccg
     1681 aaggcgccgc cgtcccccaa gacgccgcct tcctcgaaga cgccgccccc ggtcgccacg
     1741 ggcgcgccgg ccgcttcgaa ggccgttccg gcgccgtcgc ccgccgcgct cccgccgctg
     1801 gagcccgctc cgatcgcgga gtcccggccc gcgccccccg cgcctccggc ggagcccctg
     1861 atgaagcgat cggtgaacct gaggttcgtc gcgctgatca tcgccctcgt cgccgtcgcc
     1921 ggcgccgtcg tgacgggcgt cctctggtcc cagaagaaga aggtcgaggc ccaggcggcc
     1981 gtcgacgagc tccgagagaa ggaagttcgg gagcagaagg cgctcatgga cgaggggacc
     2041 aggctcctcg acgccggcca gccgtcggcg gccctcgaga agttccggga gctcgtccgg
     2101 cgcaagccgg attccgaggc ggtccggacg gccatcgcca gggccgagga gctggtccgg
     2161 gcgcagcagt ccggagagga gctctcccgc gaggtcgaaa ctcggatggc gaccgcgcgg
     2221 gaggcgacgg agagcggcga gttcgaccgg gcgctcgacc agctggctgc ggtcctgctt
     2281 ctccagccgg agaacgccga ggcgctcgcc ctccgcgagt ccgtccgcac ggcgcaagcc
     2341 gagcagacgg ccgcgcagaa gaaggcggcc gccgaagccg cccgccgcaa gcgccccacc
     2401 cccgtcccga cggccacacc gctcccgcgg gtcgccctcc cggccgaggc gcccagggcc
     2461 gcgaccccga cgccgggtgt cgcgcgcctg cgcatcgtct tccagagccc ggcccccgag
     2521 gggtacgtca tgatccggcg gaacgacaag gaggtcttcc gcaggaactt cgacttcggg
     2581 cgcaagagcg ccggcggcct cgtcgaaggg acgatcgagg tgccttcggg cggcgcgagc
     2641 tacaaggcct gggccatccc gacgaaccgg tcgttcaacg ggtacaaggc ggtcgacctc
     2701 gtcgttccgg gcggcgaaac gcagacgctc ctcctcgagc tgaatgcgga aaaagagctc
     2761 gtcgtccggc tccgttaggc cgctccttct cgcgctcgcg tccttcccgc tcttcgtcgc
     2821 ggtcgcccgc ggcgacgagc gggcgtcgtt cggaggcgcc gggcatcagg cgccggcccc
     2881 cgtcctgacc tcgagccgcg gcgtcgtcgt ctccgcagcg ccggaggcgt cgcaggcggg
     2941 cgccgaggtc ctcgcggcgg gcggaaacgc gatggacgcc gctctggcgg cggcgttcgc
     3001 tctcgcggtg gtctatcccc aggcgggcaa cctcgccggg gggggcttcc tggtcgtccg
     3061 gacgccggac ggagtcgttc gcgcgctcga cttccgggag acggccccgg ccggcgcgac
     3121 gcgcgagatg ttcctcggac cggacggccg gccggtgacc ggatcgtcga cggagacgat
     3181 gctcgcggtc gccacgcccg ggtccgtccg tggctacgcg gaggcgcatc gcctcctcgg
     3241 acgccttccc tgggccaagg tcgtcgcgcc cgcggagcgt ctcgcgcgcg aaggattcgt
     3301 c
//
LOCUS       DAOTEZ010000468         3273 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_22456_length_3273_cov_5.044748, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000468 DAOTEZ010000000
VERSION     DAOTEZ010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22456_length_3273_cov_5.044748"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="P5164_17915"
     CDS             complement(<1..806)
                     /locus_tag="P5164_17915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY45812.1"
                     /translation="MSATAAARTERLTVRYGRTIAVDAVSVDVPKGSVFALLGRNGAG
                     KSSLLRCLLGWQKATDGRCELLGDDPWKRRARLMERVGVVPEEPDAPREMTPRQLGAF
                     CARLYPSWDAKGYDARLARFGVPPSTAFGRLSKGQKGLTQLSLALASSPDLLLLDDPT
                     LGLDPVARHAFFDEIVAELADRGTTVFLTTHDLAGVERIADRVGILKDGRLLLHEETE
                     ALKSRFRAVRYRSEVTEGRTDFGTELDELEPVRVKAEATASAFSDERFER"
     gene            complement(803..1213)
                     /locus_tag="P5164_17920"
     CDS             complement(803..1213)
                     /locus_tag="P5164_17920"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRY45813.1"
                     /translation="MTPRGLRVDPRDAVPIWKQIEEGMRRLVASGALAPGTAVPSVRD
                     LARDLQVNPATVVRAYQRLVEAGLFVVRRGEGTFVAEAPPSLPKPERARALAEGALRF
                     ASTAVTVGASRDEAARALESAFARLAPTKEGEKK"
     gene            complement(1459..>3273)
                     /locus_tag="P5164_17925"
     CDS             complement(1459..>3273)
                     /locus_tag="P5164_17925"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRY45814.1"
                     /translation="DNFFLEGQYSTRKFTFEGSGSRFTDKIFGTLMVNNANGRRWWSP
                     TFCGVCEDEKRDADQALLKGTYFLSTKSLGSHSIVLGGEMYDDQRFSNNHQSGSDYRI
                     YVTNVLASGGQIYPVVNTTASGNSLSFIRWTPILQGSQGNSFKTWSVFLNDVWALNKN
                     LSFNVGVRWDKNDGQDGTGRKTVDSDAISPRLGGTWDVKGDGDMIVSAAYARYVAGIN
                     NSQGDASSLGGQPATVDFDYRGPTFNLDPNGPLTSTEEVIRQVFAWFEANGGTSRPVR
                     GIPSIPGINPNIEGTLYSPNTDEFSLGLKKNLGTRGMVRGDIVYRTWNDFYGLRVDMG
                     TGTVSGTLAGVTRTFNKQLVVNTDEEERKYWGFTLSAAYRPIDGLQLQGNWTWSRLRG
                     TFDGENAASGSLQSLAPYYPEYKQASWNRPAGDLGADQRHKIRLWAIYDLPLGIDWMQ
                     TSVSLLQNFDSGLPYGAVGAVVSTPYVTNPGYLSVPANHTYYFTARDAFKTDDVWRTD
                     LSLNLSFRPFGSVEVFASPQVLNVFNQQAQTGVTTTIETAQNTTGYAAFNPFTTAPVQ
                     GARNTGANWNYSSSFGAPVSPASFQQPRTFRFSVGLRF"
BASE COUNT          473 a         1026 c         1269 g          505 t
ORIGIN      
        1 aagcgctcga agcgctcgtc ggagaaggcc gaggccgtcg cctcggcctt cacgcggacc
       61 ggctcgagct cgtcgagctc ggtcccgaag tcggtccgcc cctcggtcac ctcgctccgg
      121 tagcggacgg cgcggaaccg ggacttcagc gcctccgtct cctcgtggag gaggagccgc
      181 ccgtccttca ggattccgac gcggtcggcg atccgctcga cgcccgcgag gtcgtgcgtc
      241 gtcaggaaga ccgtcgtgcc ccggtcggcg agctccgcga cgatctcgtc gaagaacgcg
      301 tgccgcgcga ccgggtcgag gccgagcgtc ggatcgtcca ggaggagaag gtcgggggag
      361 gaggcgaggg cgagagagag ctgcgtcagc cccttctgcc ccttggagag ccggccgaag
      421 gcggtcgagg gcggcacgcc gaatcgcgcc agccgggcgt cgtagccctt tgcgtcccac
      481 gacgggtaga ggcgcgcaca gaaagcgccc agctgccggg gcgtcatctc gcgcggggcg
      541 tccggctcct cggggacgac gccgacgcgc tccatgagcc gcgcacggcg cttccagggg
      601 tcgtcgccga gaagctcgca gcggccgtcc gtcgccttct gccagccgag gaggcagcgg
      661 agaagagacg acttgccggc gccgttccgc ccgaggagcg cgaagacgga gcccttcggg
      721 acgtcgacgg agaccgcgtc gacggcgatc gtccggccgt agcggaccgt cagccgctcg
      781 gtgcgggccg cggcggttgc gctcacttct tctctccttc cttcgtgggg gcgaggcggg
      841 cgaaggcgct ctcgagggcg cgcgcggcct cgtcgcgcga ggcgccgacg gtgacggccg
      901 tcgaggcgaa ccgaagcgcc ccttcggcga gggctcgggc gcgctccggc ttcgggaggg
      961 acggaggcgc ctcggcgacg aacgtcccct cgccgcggcg gaccacgaag aggcccgcct
     1021 cgacgagccg ctggtaggcg cggacgaccg tcgcggggtt cacctggagg tcgcgggcga
     1081 ggtcgcggac ggacgggacc gccgtgccgg gcgcgagcgc gcccgaggcg acgagccgcc
     1141 gcatcccttc ctcgatctgc ttccagatcg ggacggcgtc gcgcgggtcg acgcggaggc
     1201 cgcggggggt cacggggagc tcctggcgct tcggtgtatc acaacaccga tacggtagga
     1261 cggctccggg gggctgtcaa gggaaacgtg cgagtccggg gcggcgtgtc gctccgggac
     1321 cgagccggcg gaccggccga gcgttcccgg gcggggccga gaaggagagg acgccccgaa
     1381 agcgaagagc cccggccgcg ggccggggct ctttcgggaa gccggagcgc cggacgagcc
     1441 ggcgctccgg acgagtcgct agaagcggag gccgacggag aagcggaacg tccgcggctg
     1501 ctggaagctc gccgggctga cgggggcgcc gaacgaggag gagtagttcc agttcgcgcc
     1561 ggtgttcctc gcgccctgga cgggggcggt cgtgaagggg ttgaaggcgg cgtatccggt
     1621 cgtgttctgg gcggtctcga tcgtcgtcgt gacgcccgtc tgcgcctgct ggttgaagac
     1681 gttcaggacc tgcggcgagg cgaagacttc gacggagccg aagggccgga aggagaggtt
     1741 cagggagagg tcggtcctcc agacgtcgtc ggtcttgaag gcgtcgcgcg ccgtgaagta
     1801 gtaggtgtgg ttcgccggga cgctcaggta gcccgggttc gtgacgtagg gggtcgaaac
     1861 gaccgccccg acggcgccgt acgggaggcc ggagtcgaag ttctggagga ggctgacgga
     1921 ggtctgcatc cagtcgatcc cgagcggcag gtcgtagatc gcccagaggc ggatcttgtg
     1981 gcgctggtcg gcgccgaggt cgccggcggg gcggttccac gaagcctgct tgtactcggg
     2041 gtagtagggg gcgaggctct ggagcgagcc gctcgcggcg ttctcgccgt cgaacgtgcc
     2101 gcgcaggcgg gaccaggtcc agttcccctg gagctggagg ccgtcgatcg gacggtaggc
     2161 cgcgctcagc gtgaagcccc agtacttccg ctcttcctcg tcggtgttga cgacgagctg
     2221 cttgttgaat gtgcgcgtca cgccggcgag cgtcccggag acggtcccgg tccccatgtc
     2281 gacccgcagg ccgtagaagt cgttccaggt ccggtagacg atgtcgccgc ggaccatgcc
     2341 gcgagtgccg aggttcttct tcaggccgag ggagaactcg tcggtgttcg gcgagtagag
     2401 cgtcccctcg atgttcgggt tgatgccggg gatggacggg atgccgcgga cggggcggct
     2461 cgtcccgccg ttcgcctcga accaggcgaa gacctggcgg atgacctctt cggtcgaggt
     2521 gagcgggccg ttcgggtcga ggttgaacgt cggcccgcgg tagtcgaagt cgaccgtggc
     2581 gggctggccg ccgagggagg aggcgtcgcc ctgcgagttg ttgatgccgg cgacgtagcg
     2641 ggcgtacgcg gcgctgacga tcatgtcgcc gtcacccttg acgtcccagg tcccgccgag
     2701 gcgggggctg atcgcgtcgc tgtctacggt cttccggccc gtgccgtcct ggccgtcgtt
     2761 cttgtcccaa cggacgccga cgttgaagga gaggttcttg ttgagggccc agacgtcgtt
     2821 caggaagacg gaccaggtct tgaacgagtt cccctggctc ccctggagga tcggcgtcca
     2881 gcgaatgaag gagagcgagt tgccgctggc cgtggtgttc accaccgggt agatctgacc
     2941 gccggaggcg aggacgttcg tgacgtagat ccggtagtcg cttcccgact ggtggttgtt
     3001 cgagaagcgc tggtcgtcgt acatctcgcc gccgaggacg atgctgtgcg acccgaggct
     3061 cttcgtggag aggaagtagg tccccttcag gagggcctgg tcggcgtccc gcttctcgtc
     3121 ctcgcagacg ccgcagaacg tgggcgacca ccagcggcgg ccgttcgcgt tgttcaccat
     3181 gagggtgccg aagatcttgt cggtgaagcg cgagccggag ccctcgaacg tgaacttccg
     3241 cgtcgagtac tgcccctcga ggaagaagtt gtc
//
LOCUS       DAOTEZ010000469         3272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_22462_length_3272_cov_6.256139, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000469 DAOTEZ010000000
VERSION     DAOTEZ010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22462_length_3272_cov_6.256139"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="P5164_17930"
     CDS             complement(<1..394)
                     /locus_tag="P5164_17930"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45815.1"
                     /translation="MRRRIGDVLVGGKAISQELRDRALAQQVKQKGRLCTNLLELGGV
                     REDLLLRALAVQYGVATCTAADLAEIRPDILRLVPAKLAANLFVVPFKRLGRNLSLAM
                     RDPKDLPAIDEVSFLTGLAITPFVALDIR"
     gene            446..865
                     /locus_tag="P5164_17935"
     CDS             446..865
                     /locus_tag="P5164_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HRY45816.1"
                     /translation="MTGRPLRVLIAKPGLDGHDRGAKVVARALRDAGMEVIYTGLRQT
                     PEQVAAAAVQEDVDVVGLSILSGAHNVLVPEVISALGRRGAEGVSVVVGGIIPEKDIP
                     GLTAAGVKAVFPPGTTTSAVIESVRGLGRPLEAAAAR"
     gene            936..2549
                     /locus_tag="P5164_17940"
     CDS             936..2549
                     /locus_tag="P5164_17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRY45817.1"
                     /translation="MSDVLVSRVDSTSEDYLQNRAHHEGLARELRERVARAREGGGTK
                     GAARQAELGKLSVRGRIERLLDRETAFLELSPLAADGMFDGQAPGAGLVTGIGRVSGR
                     DVMVVANDPSVKGGTYFPITVKKHLRAQEVALENRLPCIYLVDSGGAFLPLQAEVFPD
                     RDHFGRIFYNQARMSAAGIPQVSAVLGSCTAGGAYVPAMSDEVVIVKGQGTIFLAGPP
                     LVKAATGESVTAEELGGGDVHTRISGVADFLASSDAEALEMVREIVARLNTVKTFSGD
                     VAQPEEPLYAPEELYGVLPRDLKRPLEIREVLARLLDGSRLHEFKPRYGPTLVCGFGR
                     LHGMPVGVLANNGVLFSESALKGTHFIEMACQRRVPLLFVQNITGFMVGKAYEHGGIA
                     KDGAKMVHAVANAAVPKLTLLVGGSFGAGNYGMCGRAYQPRFLFSWPGSRISVMGGEQ
                     AAGVLSTVKNEQRERQGQAPMTPEEEAAFKKPTLEKYESEGSPYYATARLWDDGILDP
                     AETRKVLSLALSVCHNAPIPETSFGVFRM"
     gene            2558..>3272
                     /locus_tag="P5164_17945"
     CDS             2558..>3272
                     /locus_tag="P5164_17945"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HRY45818.1"
                     /translation="MKRACSVPLLLVSLLAALSVPAAGTPKGRVRSVEVTSLTRQKAF
                     LFVRTTLPSAARGSTQKFRGTATAWGVSVPLVQPVKVAVQRSGEGFDAVFLAEIDLAA
                     VPKGIVEKGSATALPVALKGTLAGEAGTAVTVDAAGALRPGTADLVAHRELGDGFVRF
                     AGSRISGVGLAETKGEARLAVFNPFRFDVSLRGLRYELASGGRAVARGSRQGVIVRPT
                     RENEIALPLTVSNAAALAAA"
BASE COUNT          456 a         1170 c         1172 g          474 t
ORIGIN      
        1 tccggatgtc gagggcgacg aagggtgtga tcgcgagccc cgtcaggaag gagacctcgt
       61 cgatcgccgg gaggtccttc gggtcgcgca tggcgaggga gaggtttctc ccgagcctct
      121 tgaacgggac gacgaagagg ttcgcggcga gcttggccgg gacgagccgg aggatgtcgg
      181 ggcggatctc ggccaggtcg gcggccgtgc aggtggcgac tccgtactgc acggcgagcg
      241 cccggaggag caggtcctcg cggacgccgc ccagctcgag gaggttcgtg cagaggcgcc
      301 ccttctgctt cacctgctgg gccagggcgc ggtcccgcag ctcctgggag atggcctttc
      361 ccccgacgag aacgtccccg atccggcgcc gcatgcggag aatctagcag gaaccggacc
      421 ggaggccggt tagaattcca tcgccatgac gggaagacca ctccgagtcc tcatcgccaa
      481 gcccggcctc gacggccacg accgcggcgc caaggtcgtc gcgcgtgccc tgcgcgacgc
      541 cgggatggaa gtcatctaca ccggcctccg gcagaccccc gagcaggtgg ccgccgcggc
      601 cgtccaggaa gacgtcgacg tcgtcggcct ctccatcctc tccggcgctc acaacgtcct
      661 cgtccccgag gtgatctcgg cgctcgggcg gcgcggcgcc gaaggcgttt cggtcgtcgt
      721 cggcgggatc atccccgaga aggacatccc cggcctgacg gccgccggcg tgaaggcggt
      781 cttcccgccc ggaacgacca cgagcgccgt catcgagtct gtccgaggcc tcggccggcc
      841 gctcgaagcc gccgccgctc gctgacgacg gcggatgccg ggcgcgccgc ggaggcgtgc
      901 cccgggtccg acggtccggc cgctagaatc gcccggtgag cgacgttctc gtcagccggg
      961 tcgattccac gtccgaggac taccttcaga accgggccca ccacgagggc ctcgcccgcg
     1021 agctgcgcga gcgggtcgcc cgggcgcgcg aagggggcgg cacgaagggc gccgcgcgcc
     1081 aggcagagct cgggaagctc tccgtgaggg ggcggatcga gaggctcctc gatcgcgaga
     1141 ccgcgttcct cgagctgtcg cccctcgccg cggacgggat gttcgacggg caggcgcccg
     1201 gcgcggggct cgtcaccggg atcgggcggg tctcggggcg ggacgtgatg gtcgtcgcga
     1261 acgacccgtc ggtgaaggga gggacctact tcccgatcac cgtgaagaag cacctccgcg
     1321 cccaggaggt cgccctcgag aaccgcctcc cgtgcatcta cctcgtcgac tcgggcgggg
     1381 cgttcctgcc gctgcaggcc gaggtcttcc cggatcgcga ccacttcggc cggatcttct
     1441 acaaccaggc ccgcatgtcg gcggccggca tcccgcaggt ctccgccgtc ctcggctcct
     1501 gcaccgcggg gggcgcctac gtcccggcga tgtcggacga ggtcgtcatc gtgaaggggc
     1561 aggggacgat cttcctcgcg ggtccgcccc tcgtgaaggc ggcgacgggc gagtcggtca
     1621 cggccgagga gctcggcggg ggagacgtcc acacgcggat ctcgggcgtg gccgacttcc
     1681 ttgcctcctc ggacgccgag gcgctcgaga tggtccgcga gatcgtcgcg cgcctgaaca
     1741 cggtcaagac gttctcgggc gacgtcgcgc agccggagga gccgctctac gcgcccgagg
     1801 agctctacgg cgtcctcccg cgcgacttga agcggcctct cgagatccgc gaggtcctcg
     1861 cgcgtctcct cgacggctcg cgcctccacg agttcaagcc ccgctacggg ccgacgctcg
     1921 tctgcggctt cgggcgcctc cacgggatgc cggtcggcgt cctcgcgaac aacggagtcc
     1981 tcttctccga gtcggccctc aaggggacgc acttcatcga gatggcctgc cagcggcggg
     2041 tcccgctcct cttcgtccag aacatcaccg gcttcatggt cgggaaggcg tatgagcacg
     2101 gcggcatcgc gaaggacggc gcgaagatgg tccacgccgt cgcgaacgcc gccgtcccga
     2161 agctgacgct gctcgtcggc ggctcgttcg gcgccggcaa ctacggcatg tgcggccgcg
     2221 cctaccagcc gcgcttcctc ttcagctggc ccggctcgcg gatcagcgtc atgggggggg
     2281 agcaggccgc cggagtcctc tcgaccgtca agaacgagca gcgcgagcgc caggggcagg
     2341 ccccgatgac tccggaggag gaggccgcgt tcaagaagcc gacgctcgag aagtacgagt
     2401 ccgaggggag cccgtactac gccacggccc ggctctggga cgacgggatc ctcgacccgg
     2461 ccgagacccg gaaggtcctc tccctcgccc tctcggtctg ccacaacgcg ccgatcccgg
     2521 agacgtcgtt cggcgtcttc cggatgtgag gagcgacgtg aagcgagcct gctccgttcc
     2581 gctcctcctc gtctctctcc tcgcggcact ttccgtgccg gccgcgggca cgccgaaagg
     2641 gagggtccgt tcggtcgagg tcacctccct cacgcggcag aaggccttcc tcttcgtccg
     2701 gacgacgctc ccgagcgcgg cgcgggggtc gacgcagaag ttccggggca cggcgacggc
     2761 ctggggcgtt tccgttcctc tcgtccagcc cgtgaaggtg gccgtgcagc gttcgggcga
     2821 aggcttcgac gccgttttcc tcgcggagat cgacctcgcg gccgtgccga agggaatcgt
     2881 cgagaagggg agcgcgacgg ccctgcccgt cgcgttgaag gggacgctcg ccggcgaggc
     2941 cgggaccgcc gtgaccgtcg acgcggccgg cgcgctccgg cccggcaccg cggacctcgt
     3001 cgcgcaccgc gagctcggcg acgggttcgt ccgattcgcc gggtcccgca tctcgggcgt
     3061 cggcctggcc gagacgaaag gcgaggcccg gctggcggtc ttcaacccgt tccgcttcga
     3121 cgtctccctc cgggggctcc gctacgagct ggcttcgggc ggccgagcgg tcgcgcgcgg
     3181 aagccgtcag ggggtcatcg tccgcccgac tcgcgagaac gagatcgccc ttccgctgac
     3241 cgtctcgaac gccgccgcgc tcgccgccgc gg
//
LOCUS       DAOTEZ010000470         3271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_22473_length_3271_cov_4.725435, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000470 DAOTEZ010000000
VERSION     DAOTEZ010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22473_length_3271_cov_4.725435"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="P5164_17950"
     CDS             <1..331
                     /locus_tag="P5164_17950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45819.1"
                     /translation="GRVAERNALDAPWIRSLDFHYDFELPISVVKTQITFDVLNLINL
                     VDNNSGLLRYVSNQTYSALNYSGIDSATGKPRYTVNANALDEGRQYSTNDLRSRWQIR
                     LGARLTY"
     gene            complement(432..956)
                     /locus_tag="P5164_17955"
     CDS             complement(432..956)
                     /locus_tag="P5164_17955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HRY45820.1"
                     /translation="MSAASKGGAFPEEGGVRLLDGGVLDALSAEARSRPRRRANGNLH
                     AMEDPVHRLLNAVEPGTYIRPHRHLHPPKDETYVVVRGALGLVLFDASGGVTAARRLS
                     VGTGEAFGADIPAGVLHTVVALEEGTVFFEVKVGPYVPSAGDDVPAWAPAEGEPAAVA
                     YEESLRALFAPVAR"
     gene            complement(953..1816)
                     /locus_tag="P5164_17960"
     CDS             complement(953..1816)
                     /locus_tag="P5164_17960"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HRY45821.1"
                     /translation="MNARALPLVAAGLALAALAPSAAAQPRLPFPSPAATVSQEIGTS
                     LVTIEYHRPAVRGREIWGALVPYGETWRLGANEATTLTLSHGAKVAGHDVPAGTYALF
                     AVPGKERWTLVLNRKAKQWGAYFTDSKEDLLRFEVTPAAAPPVERMTFALEPVEPGTV
                     RVSWAWEKLAFSFPVEFDVAAIVWAGVDAALKEKPDDMGTLLQAARWALQRKERLAEG
                     RAWIDRSVAVKESFWNRELLANYLRHEGKTAEAVVAMKRAIELSKGKAPDGYWKALEA
                     TVAAWEAEKAP"
     gene            complement(1813..>3271)
                     /locus_tag="P5164_17965"
     CDS             complement(1813..>3271)
                     /locus_tag="P5164_17965"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRY45822.1"
                     /translation="PRKADVINLETRQFETVDLETLFREAGDALPGLEEMVSVHEEGR
                     IHRPLMVDWQERARDLVVTFEGLVAGTPFVARIRALLRLLQEKLGGPVDVEFASDGKD
                     LYLLQCRPQSFADESQAAAIPTDVPAERIVFSANRFVSNGRVPDVTHVVYVDPEAYAA
                     IPSLDTLREVGHAVGRLNKLLPRRQFVLMGPGRWGSRGDIKLGVAVTYSEINNAAVLI
                     EIARRKGSYVPDLSFGTHFFQDLVEAQIRYLPLFPDDPGVAFNERFLLDSPNLLPSLV
                     PEAAHLAKTLRVIDVPAATGGLVLKVRMNADLDKAVAILAEPGAPEKGAGGVRQAAIG
                     IPGSAGADDDRRWRIHLAERIAAETDPERFGVKGMWLVGSSHDGTAGPGSDVDLVVHT
                     DDDPARREALATWLEGWSLCLGEVNYLRTGLRTKGLLDVRFISDSDISRGTSWAAKVS
                     AVKDPARPLPLHASVRTGPVPRRGRGAAKNEGGPR"
BASE COUNT          458 a         1193 c         1157 g          463 t
ORIGIN      
        1 cggccgcgtg gctgagcgca acgcgctcga cgccccctgg atccgcagcc tcgacttcca
       61 ctacgacttc gagctcccga tctcggtcgt caagacgcag atcacgttcg acgtcctcaa
      121 cctcatcaac ctcgtcgaca acaactccgg gctcctccgc tacgtctcca accagacgta
      181 cagcgccctc aactactccg gcatcgacag tgccaccggc aagccgcgct acacggtcaa
      241 cgccaacgcg ctcgatgaag gccggcagta cagcacgaac gacctccgct cgcgctggca
      301 gatccgcctc ggcgcgcgcc tgacctactg atccttccgg agcgtccgga acgagcgggg
      361 ggccgaaagg ccccccgttt cctttgcccc ccggctgcga cggggcccgc gcggacccgg
      421 gcgtgaacgc cttaccgcgc gaccggcgcg aagagggccc ggagcgactc ctcgtaggcc
      481 acggcggccg gctccccttc cgcgggcgcc cacgccggca cgtcgtcgcc ggccgaaggg
      541 acgtacgggc cgaccttcac ctcgaagaag accgtcccct cctcgagcgc gacgacggtg
      601 tggaggactc ccgcggggat gtccgctccg aaagcctccc ccgtcccgac ggagagccgt
      661 cgggcggccg tcaccccgcc cgacgcgtcg aagagaacaa ggccgagggc gccccggacg
      721 acgacgtagg tctcgtcctt cggcgggtga aggtgccggt gggggcggat gtaggtgccg
      781 ggctccaccg cgttgagcag gcggtggacc gggtcctcca tcgcgtggag gttcccgttc
      841 gcgcggcgcc gcgggcggct ccgggcctcc gccgagagcg cgtcgaggac gcctccgtcg
      901 aggaggcgga cgcctccctc ctcgggaaag gccccaccct tcgacgccgc gctcacggcg
      961 ccttctccgc ttcccacgcc gcgacggtcg cctcgagcgc cttccagtag ccgtcggggg
     1021 ccttcccctt cgagagctcg atggcccgct tcatcgcgac gaccgcctcg gccgtcttcc
     1081 cctcgtgccg gaggtagttc gcgaggagct cgcggttcca gaagctctcc ttcacggcga
     1141 cggagcgatc gatccaggcc cgtccctccg cgagccgctc cttccgctgg agcgcccagc
     1201 gggcggcttg caggagcgtc cccatgtcgt ccggcttctc cttcaacgcg gcgtcgacgc
     1261 cggcccagac gatcgcggcg acgtcgaact cgaccgggaa ggagaaggcg agcttctccc
     1321 aggcccagga gacgcggacc gtgcccggct cgacgggctc gagcgcgaag gtcatccgct
     1381 cgacgggcgg cgccgcggcc ggcgtcacct cgaagcggag gaggtcctcc ttcgagtctg
     1441 tgaagtaggc gccccactgc ttcgccttgc ggttcaggac gagcgtccag cgctcctttc
     1501 ccgggacggc gaagagggcg tacgtgccgg ccggcacgtc gtgaccagcg accttcgcgc
     1561 cgtgagagag agtcagcgtc gtcgcctcgt tcgcgccgag tcgccacgtc tcgccgtacg
     1621 ggacgagggc gccccagatc tcgcgtccgc ggacggcggg ccggtggtac tcgatcgtga
     1681 cgagggacgt cccgatctcc tgggagaccg tcgccgcggg gctcgggaac gggagccgcg
     1741 gctgcgccgc ggcggacggg gcgagcgccg ccagcgcgag gccggcggcg acgagcggaa
     1801 gtgcgcgtgc gttcatcgag ggcctccttc gttcttcgcg gcgccgcgtc ccctccgggg
     1861 cacgggaccg gtccggaccg aggcgtggag cggcagcggc cgcgccgggt ccttcacggc
     1921 ggacaccttc gcggcccagc tcgtcccgcg ggagatgtcg gagtcggaga tgaagcggac
     1981 gtcgaggagc cccttcgtcc ggagtcccgt ccgcaggtag ttcacctccc cgaggcaaag
     2041 gctccacccc tcgagccacg tcgcgagggc ctcgcgccgg gccgggtcgt cgtcggtgtg
     2101 gacgacgagg tcgacgtcgc tccccggccc cgccgtcccg tcgtgcgagg agccgacgag
     2161 ccacatcccc ttaaccccga agcgctccgg gtcggtctcg gcggcgatcc gctcggcgag
     2221 gtggatcctc cagcgccggt cgtcgtccgc gccggccgat ccggggattc cgatcgcggc
     2281 ctggcggacc cctcccgccc ccttctccgg cgctcccggc tcggcgagga tcgcgacggc
     2341 cttgtcgagg tcggcgttca tccgaacctt caggacgagg ccgcccgtcg ccgccggcac
     2401 gtcgatcacg cggagggtct tcgcgaggtg cgcggcctcg gggacgagag acggcaggag
     2461 gttcggcgag tcgagcagga agcgctcgtt gaacgcgacg ccggggtcgt ccgggaagag
     2521 cgggagatag cggatctggg cctcgacgag gtcctggaag aagtgggtgc cgaaggagag
     2581 gtcggggacg tagctcccct tgcggcgggc gatctcgatc aggaccgccg cgttgttgat
     2641 ctcggagtag gtcaccgcga cgccgagctt gatgtcgccg cggctccccc accgccccgg
     2701 ccccatcagg acgaactggc gccgcggaag gagcttgttc agccgcccga cggcgtgccc
     2761 gacctcgcgg agggtgtcga ggctcgggat cgcggcgtag gcctcggggt cgacgtagac
     2821 gacgtgggtg acgtcgggaa cgcggccgtt cgagacgaag cggttcgccg agaagacgat
     2881 ccgttccgcc gggacgtcgg tcgggatcgc cgccgcctgg ctctcgtcgg cgaagctctg
     2941 cgggcggcac tggaggaggt agaggtcctt cccgtccgag gcgaactcga cgtcgaccgg
     3001 gcccccgagc ttctcctgca ggagcctcag gagcgcccgg atccgcgcga cgaacggggt
     3061 ccccgcgacg agcccctcga acgtgacgac gaggtcgcgc gcccgctcct gccagtcgac
     3121 catgagcggg cggtggattc gcccctcctc gtggacggag accatctcct cgaggcccgg
     3181 cagggcgtct cccgcctcgc ggaagagggt ctcgaggtcg accgtctcga actgccgcgt
     3241 ctcgaggttg atgacgtccg ccttccgggg c
//
LOCUS       DAOTEZ010000471         3259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_22584_length_3259_cov_7.135144, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000471 DAOTEZ010000000
VERSION     DAOTEZ010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22584_length_3259_cov_7.135144"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1701)
                     /locus_tag="P5164_17970"
     CDS             complement(<1..1701)
                     /locus_tag="P5164_17970"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRY45823.1"
                     /translation="MAKTILLIEYEPRYLDRIKGYLAGKDFELVVAKDGEDGLEAYRR
                     AKPDLVLISTVLPKLRTRDVIRGMQGLGATPPILLMASGYKGKDKKADAAREGASGIL
                     EKPFSEEALLGEISSALGGGAAEPFAAAGAQPLLSSDDIFSDVISGVEAEEKARPGTP
                     AGTDPGINRKLEQTLSGILSRPGAKPAPAPAPAPKPAAPPAAVPAPRVEANPPSQPIP
                     APKPKVDTAVDRMLTETLSGLRPVKPAEKPAPAPAPVAAPAPAPKPAPAPAPKPEPAP
                     APKAAPAPKAKPAAGGPGSFGRYQLLEKIAAGGMAEVYKARMRGEEGFEKIVAIKRIL
                     PHMADNDDFITMFIDEAKLAAQLTHNNIIHIYDLGKEDAYHYIAMEYVEGKDLRSILK
                     QGSEKGFPLPVELALFVASKVANALDYAHRRMGMDGKELNLVHRDVSPQNVLISFEGD
                     IKLCDFGIAKAATKVQQTQAGALKGKLQYMSPEQAWGKKVDRRTDIFSLGVVLYEMLT
                     GERLFSGDTDLTILEQVRDARSTPPSAKNADVPAKVDQIVLKALAKNPQDRYQNASEM
                     E"
     gene            1779..3131
                     /locus_tag="P5164_17975"
     CDS             1779..3131
                     /locus_tag="P5164_17975"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HRY45824.1"
                     /translation="MSLSTKFGFAERVGKRSGRAYAVHVLGCKVSQIEADGIARTLES
                     EGYEPASPGEPAEVVVLHACTVTARADRDALRQLRRLRRENPAALLAVTGCLAERDPE
                     GLSRRPEVDVVAGHGSRLALPRLLEDAAAGLLPGKVADRTSSPAEVLASPFAAGGPDR
                     TRAFLKIQDGCGRRCAFCIVPSLRGAERSAPDGEVEDAIRSLGEAGVPEVVLCGVHLA
                     AFGHDRGGSLLGLLRRLEARPPACRLRLSSLEPMEAGEDLVDLVASSRVVAPHLHLPL
                     QSGSDAVLRRMRRGMTRARYRALLLRAARANPRVHLATDVIAGFPGETDAEFAETEAL
                     LAELPLASLHVFPFSPRSGTAAAALAETGSVPAPLRTRRAARLRALDAALRRRFAEAH
                     AGRAADVVALRGGVGLTETYVEVALPPGGPPPGSRFAALLALRPDGRLEARPAVPFSC
                     "
BASE COUNT          374 a         1200 c         1167 g          518 t
ORIGIN      
        1 ctccatctcc gaggcgttct ggtagcggtc ctgcgggttc ttcgcgagcg ccttgaggac
       61 gatctggtcg accttcgccg ggacgtccgc gttcttcgcc gacgggggcg tcgagcgcgc
      121 gtcgcggacc tgctcgagga tcgtcaggtc ggtgtcgccg ctgaagagcc gctcgcccgt
      181 cagcatctcg tagaggacga cgccgagcga gaagatgtcg gtgcggcggt cgaccttctt
      241 cccccacgcc tgctcggggg acatgtactg gagcttgccc ttgagggcgc ccgcctgcgt
      301 ctgctggacc ttcgtcgcgg ccttcgcgat gccgaagtcg cagagcttga tgtccccctc
      361 gaagctgatg aggacgttct gcggggagac gtcgcggtgg acgaggttca gctccttgcc
      421 gtccatcccc atgcgacggt gggcgtagtc gagggcattg gcgaccttcg aggcgacgaa
      481 gagcgcgagc tcgaccggga gcgggaaccc cttctccgag ccctgcttca ggatggagcg
      541 gaggtccttg ccctcgacgt actccatcgc gatgtagtgg taggcgtctt ccttccccag
      601 gtcgtagatg tggatgatgt tgttgtgggt cagctgcgcc gcgagcttgg cctcgtcgat
      661 gaacatcgtg atgaagtcat cgttgtcggc catgtgcggg aggatccgct tgatcgcgac
      721 gatcttctcg aaaccctcct cgccgcgcat gcgcgccttg tagacctcgg ccattccgcc
      781 ggcggcgatc ttctcgagga gctggtagcg cccgaagctg ccggggccgc cggcggccgg
      841 cttcgccttc ggcgcggggg cggccttcgg cgccggggcg ggctcgggct tcggcgccgg
      901 ggccggcgcc ggcttcgggg ccggcgcggg agcggcgacg ggagcggggg ccggtgcggg
      961 cttctcggcc ggcttgaccg gccggagacc ggagagcgtc tcggtgagca tacggtcgac
     1021 ggcggtgtcg accttcggct tcggagccgg gatcggctgc gagggcgggt tcgcctccac
     1081 gcgcggcgcc gggacggccg cggggggcgc ggccggcttc ggggccgggg cgggcgccgg
     1141 ggcgggcttc gcgccgggac gggagaggat gccggagagc gtctgctcga gcttccggtt
     1201 gatccccggg tcggttccgg ccggggttcc ggggcgggcc ttctcctcgg cttccacgcc
     1261 ggagatgaca tccgagaaga tgtcgtcgga ggagaggagc ggctgcgcgc cggccgcggc
     1321 gaacggctcc gcggctcctc cgccgagcgc ggaggagatc tccccgagga gggcctcctc
     1381 ggagaacggc ttctccagga tcccgctcgc gccctcgcgg gccgcgtcgg ccttcttgtc
     1441 cttccccttg tacccggagg ccatcaggag gatcggaggc gtcgcgccga gcccctgcat
     1501 cccgcggatc acgtcgcgcg tgcggagctt cggcaggacg gtggagatca ggacgaggtc
     1561 gggcttcgcc cgcctgtaag cctcgagccc gtcctcgccg tccttcgcga cgaccagttc
     1621 gaagtccttc ccggcgagat agcccttgat gcggtcgagg tatctcggct cgtattcgat
     1681 caggagaatc gtcttcgcca tacgtcgtgg ggcctgcggc ccggtccccc ccgcactccg
     1741 aagtctcctc cggaaagtca cttattatcg ccccggaagt gagcctgtca acgaagtttg
     1801 gcttcgccga gcgcgtcggc aagcgctccg ggcgagccta cgccgtccac gttctcggct
     1861 gcaaggtgag ccagatcgag gccgacggga tcgctcgaac gctcgagtcc gaggggtacg
     1921 agcccgcgag tcccggcgag ccggcggagg tcgtcgtcct ccacgcctgc accgtgaccg
     1981 cccgcgcgga ccgcgacgcc ctccgccagc tccggcgcct tcggcgcgag aacccggcgg
     2041 ctctcctcgc ggtgaccggc tgtctcgccg agcgggaccc ggagggcctc tcccgccgtc
     2101 cggaggtcga cgtcgtcgcc ggtcacggca gccgcctcgc cctcccccgc cttctcgagg
     2161 acgcggccgc ggggctcctg cccggaaagg tcgcggatcg gacctcctcc cccgccgagg
     2221 tcctcgcgtc gcccttcgcc gcgggcggcc ccgacaggac gcgcgcgttc ctcaagatcc
     2281 aggacgggtg cggacggcgc tgcgccttct gcatcgtccc ctcgctccgg ggcgccgagc
     2341 ggagcgcgcc ggacggcgag gtcgaggacg cgatccggtc cctcggtgag gccggcgtcc
     2401 ccgaggtcgt cctctgcggc gtccacctcg ccgccttcgg gcacgaccgc ggggggagcc
     2461 tcctcggcct ccttcgccgg ctcgaggcgc gcccgcccgc ctgccggctc cggctctcct
     2521 ccctcgagcc gatggaggcg ggggaggacc tggtcgacct cgtcgcctcc tcccgcgtcg
     2581 tcgctcccca cctccacctg ccgctccagt cgggctccga cgccgtcctc cggaggatgc
     2641 ggcgcgggat gacccgagcg cggtaccggg cgctcctcct gcgggccgcc cgggcgaatc
     2701 cgcgagtcca cctcgcgacg gacgtcatcg ccggcttccc cggagagacg gacgcggagt
     2761 tcgccgagac cgaggctctc ctcgcggagc tccccctcgc gtcccttcac gtcttcccgt
     2821 tctcgccccg gtccggcacg gccgccgccg cgctcgcgga gaccggctcc gtcccggcgc
     2881 ctctccgcac ccgccgcgcg gcccggctgc gcgcgctcga cgccgcgctc cggcggcgct
     2941 tcgcggaggc ccacgccgga cgcgccgccg acgtcgtcgc gctgcggggg ggcgtcgggc
     3001 tgaccgaaac gtacgtcgag gtcgcgctcc ctccgggcgg gccgcccccc gggagccggt
     3061 tcgccgccct cctggcgctt cgtccggacg gccgcctcga ggcccgcccg gccgttccgt
     3121 tctcctgcta gactccgcgg ctcctgaatc cttggcccgc gcggcccgca ctcctcgagg
     3181 tgagccatgt ccggacacag caaatggagc acgatcaagc acaagaaggg cgccgccgac
     3241 gccaagcgcg gccggctct
//
LOCUS       DAOTEZ010000472         3252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_22651_length_3252_cov_4.574601, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000472 DAOTEZ010000000
VERSION     DAOTEZ010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22651_length_3252_cov_4.574601"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..1564
                     /locus_tag="P5164_17980"
     CDS             83..1564
                     /locus_tag="P5164_17980"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRY45825.1"
                     /translation="MSGFSSLDWVVVLLYMGGIAAFGLAKGGRQASAKDYFLSEKKIG
                     WGAVTASIVATETSALTFISVPGMAWKGAWGFLELSFGYLLGRIAVAWVLLPRYFEGE
                     LTTSYALLERSFGTGVRRLASGTFILTRIFADGVRLYATAIPLVVIFRGYNLFSGVGD
                     PTLYVGAILLTAAVTVIYVQVGGVRAVIWTDVIQLGVYVVGGFVALAVLVPKVPALGA
                     RLAEAAAAGKLTIFQTDGSFLTSPYHVLTAILGGAVLSMASHGTDYIIVQRLFATESL
                     RDGRRALVASGVVVILQFALFLAVGTLLFLFYGDLPLKGDAVFPKFIVEQVPSGLSGL
                     IIAGLLAAAMSTVSGSISSISSATVFDLWASTKRGRLASEEEKLRLSRRVSFGWAVLL
                     TGAALVFIGTGQAVVELALSIASFTYGGFLGLFLLALRGGTSRRAGTAGFLSGVGVMV
                     FVVFGTPLAWPLYTLAGSLTTVLVAAAVDRLRPAAPAEGKAGA"
     gene            1561..2232
                     /locus_tag="P5164_17985"
     CDS             1561..2232
                     /locus_tag="P5164_17985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mpv17/PMP22 family protein"
                     /protein_id="HRY45826.1"
                     /translation="MSRGSFAFCGTLASVVLLLVVPSSQEWIVETTRAHPYAMGFLKF
                     AILASLGELLAIRVVAGAWKLPAGPAWRAVVWGLLGAALALLFPVYAAGVPAAMKSGL
                     LPSLPGASGAKVATAFWTSAVMNLSWGVALMLVHRLTDTWLDLAGGALARVPSVGIGA
                     VASAIDWRGFLGFVLLKTIPFFWIPAHTVTFLLPPELRVLFAAFLSLALGAILAFAKR
                     RAAAG"
     gene            complement(2424..>3252)
                     /locus_tag="P5164_17990"
     CDS             complement(2424..>3252)
                     /locus_tag="P5164_17990"
                     /inference="COORDINATES: protein motif:HMM:NF014428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRY45827.1"
                     /translation="SDLSIDTAGPKQLTAASVGLTSAVSSAFSITSATATQVAFVQQP
                     TDALGAATIAPAVTVQLRDAAANPVAIAGVTITLALSSGTGVLSGTTSQVTNASGLAS
                     FADLSIDLAGGKQLTAASAGLASAISGAFTITPGPAAAIAVTAGSLQSAPASGAFPAP
                     LVVTVTDAGGNPVSGVTVTFLAPSSGPSALLSNGGVATTNAAGQASVTAMANGIPGGP
                     YFVTASVGAPPAAQFALTNLSADAIANVPVLGGVGIGVFALLLAAVGALASRGAFKV"
BASE COUNT          381 a         1148 c         1199 g          524 t
ORIGIN      
        1 aggagatcga ggggcacgtc cccgaggggc tcgcgacgat ggagaaggcg ttcgtgaggt
       61 tctaccgggg cgggaaggcg aggtgagcgg cttctcgtcg ctcgactggg tcgtcgtcct
      121 cctctacatg gggggcatcg ccgcgttcgg cctggcgaag ggagggcggc aggcctccgc
      181 gaaggactac ttcctctccg agaagaagat cggctgggga gcggtcaccg cctcgatcgt
      241 cgcgaccgag acctccgccc tcacgttcat ctccgtgccg ggaatggcct ggaagggggc
      301 gtggggcttc ctcgagctct ccttcggcta cctcctcggt cggatcgcgg tcgcctgggt
      361 cctcctgccg cgctacttcg agggggagct gacgacctcg tacgcgctcc tcgagcggtc
      421 gttcggaacg ggcgtgaggc gcctcgcgtc ggggacgttc atcctgaccc ggatcttcgc
      481 cgacggcgtg aggctctacg ccacggcgat cccgctcgtc gtcatcttcc gcggctacaa
      541 cctcttctcc ggggtcggcg acccgacgct ctacgtcggg gcgatcctcc tgacggcggc
      601 cgtgacggtg atctacgtcc aggtcggagg cgtccgcgcc gtcatctgga ccgacgtcat
      661 ccagctcggc gtctacgtcg tcggcggctt cgtcgccctc gcggtcctcg tcccgaaagt
      721 cccggcgctc ggcgcccggc tcgccgaggc ggccgcggcg ggaaagctga cgatcttcca
      781 gaccgacggc tcgttcctca cctcgccgta ccacgtcctg accgcgatcc tgggcggggc
      841 cgtcctctcc atggcctcgc acggcaccga ctacatcatc gtccagcgcc tcttcgcgac
      901 ggagagcctc cgcgacggcc gccgggcgct cgtcgcgagc ggcgtcgtcg tcatcctcca
      961 gttcgcgctc ttcctcgccg tcgggacgct cctcttcctc ttctacggcg acctgccgct
     1021 gaagggcgac gccgtcttcc cgaagttcat cgtcgagcag gtcccgtcgg ggctctcggg
     1081 cctcatcatc gcggggctcc tcgccgcggc gatgtcgacg gtgtcgggct cgatcagctc
     1141 gatctcgagc gccaccgtct tcgacctctg ggcctcgacg aagaggggcc gcctcgcgag
     1201 cgaggaggag aagctccgcc tctcccgccg cgtcagcttc ggctgggccg tcctcctgac
     1261 gggggcggcg ctcgtcttca tcgggacggg tcaggccgtc gtggagctcg cgctgtcgat
     1321 cgcctcgttc acctacggcg gattcctcgg cctcttcctc ctcgcgctgc ggggcgggac
     1381 gagccgccgc gcggggaccg ccgggttcct gtcgggggtc ggcgtcatgg tcttcgtcgt
     1441 cttcgggacg cccctcgcgt ggccgctcta cacgctcgcg gggagcctga cgaccgtcct
     1501 cgtggcggcg gccgtcgacc ggctccggcc ggcggccccg gccgaaggaa aggccggcgc
     1561 atgagccggg gcagcttcgc cttctgcggc acgctcgcct cggtcgtcct cctcctcgtc
     1621 gtcccgtcgt cgcaggagtg gatcgtggag acgacgcgtg cgcacccgta cgcgatgggc
     1681 ttcctgaagt tcgcgatcct ggcgagcctg ggagagctcc tcgcgatccg ggtcgtggcg
     1741 ggcgcgtgga agctccctgc cgggccggcc tggcgggcgg tcgtctgggg gctcctcggc
     1801 gcggcgctcg cgctcctctt cccggtctac gccgcggggg tcccggcggc gatgaagtcc
     1861 gggctcctgc cgtcgctccc gggcgcttcg ggggcgaagg tcgcgaccgc gttctggacc
     1921 agcgccgtca tgaacctgag ctggggcgtc gcgctgatgc tcgtccatcg cctcacggac
     1981 acctggctcg acctcgcggg cggggcgctc gcgcgggtgc cgtcggtcgg gatcggcgcg
     2041 gtggcctcgg cgatcgactg gaggggcttc ctcggcttcg tcctcctgaa gacgatcccg
     2101 ttcttctgga ttcccgcgca caccgtgacg ttcctgctgc cgccggagct gcgcgtcctc
     2161 ttcgcggcgt tcctctcgct cgcgctcggc gcgatcctcg cgttcgcgaa gcgccgcgcg
     2221 gccgcgggct gaggcgcggt ccggcccgct cggaaggacc ccggatgcgc tcggggtcga
     2281 atgcggcgca ggaagcgttc ccgcggcggg agccgcccat tcgccgtgac gccgggggcg
     2341 cgatcggtgg acgaggtcgc cgcaccccga aggcgcggag ggaaccgggc gagcccggtt
     2401 ccctcagacg cctccgtccg cgatcagacc ttgaacgcgc ctctcgacgc cagggcgccg
     2461 acggcggcga gcaggagcgc gaagacgccg atgccgacac cgccgagcac ggggacgttc
     2521 gcgatcgcgt ccgccgagag gttcgtcagg gcgaactggg ccgccggcgg cgcgcccacg
     2581 ctggccgtca cgaagtacgg cccgcccggg atgccgttcg ccatcgccgt gaccgaggcc
     2641 tgcccggccg cgttcgtcgt ggcgaccccg ccgttgctca ggagggcgct cggcccggaa
     2701 gacggagcga ggaacgtgac ggtcactccg ctcacggggt tgccgccggc atcggtcact
     2761 gtcaccacga gcggtgccgg gaaggccccg gaggccggag cgctctgaag cgatccggcg
     2821 gtcaccgcga tcgcggcggc cggaccgggc gtgatcgtga aggcgccgga aatggcggag
     2881 gccaggccgg cggaggcggc ggtgagctgc ttgccgcccg cgaggtcgat cgagaggtcg
     2941 gcaaaggagg cgaggcccga ggcgttcgtg acctggctcg tcgtgcccga gaggacgccg
     3001 gttccgctcg agagcgcgag ggtgatcgtc acccccgcga tggcgacggg gttggcggcg
     3061 gcatcccgca gctgcacggt caccgcgggg gcgatcgtgg ccgcgccgag cgcatccgtc
     3121 ggctgctgga cgaaggcgac ctgggtggcg gtcgccgaag tgatcgagaa ggcgctggag
     3181 acggcggagg tcaggccgac gctggcggcg gtgagctgct tcgggccggc cgtgtcgatc
     3241 gaaaggtcgc tg
//
LOCUS       DAOTEZ010000473         3245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_22726_length_3245_cov_4.717555, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000473 DAOTEZ010000000
VERSION     DAOTEZ010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22726_length_3245_cov_4.717555"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1676
                     /locus_tag="P5164_17995"
     CDS             <1..1676
                     /locus_tag="P5164_17995"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HRY45828.1"
                     /translation="HVVVNNQIGFTTNPKDARSSPYCTDVAKMVQAPILHVNGDDPEA
                     VVQAVDLAMEYRKAFGHDVVIDMVCYRRYGHNEGDEPSYTQPLLYAKIRNHSSVARLY
                     GDVLVREGVLTAAQVEEVWVDAKQALDRAYDASAATRTRGAFSDSLVAPAAPRYPVEG
                     SVRERLTEVVRAVSTLPPGFEVHPKLKPLLKKRADYASGRPEIDWAGGEMLAFGLLLQ
                     EGTPVRLSGQDSGRGTFSHRHSVLADPRTGAEWIPLNAIRQPQARFEVIDSFLSEAGV
                     MGFEFGYAVADPSTLVLWEAQFGDFANGAQVIIDQFISGSEQKWSQTCDLVLLLPHGQ
                     EGQGPEHSSARLERFLTLCAEDNMRVANVTTPAQYHHLLLRQMRGDVRRPLVVMSPKS
                     LLRHPKAVSRFDELAEGSFRPVLDDAAFEAGGADGVRRVVIASGKLVYELLAAREKAG
                     RSDVAIVRLEELYPFPGEELGRVIARYPKDAALVFAQEEPKNMGAWRFVMEQVLDGAV
                     PAAGGRPLRYAGRRPSASPAPGSHHVFATEQEAIAADALGVEVEVPARS"
     gene            complement(1780..2412)
                     /locus_tag="P5164_18000"
     CDS             complement(1780..2412)
                     /locus_tag="P5164_18000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45829.1"
                     /translation="MIRRKLPLLPGFALFVLVFAAAGCASTATVVPGKSLALVEVTYP
                     EMRMSGVVLDTGEATPMTRMADSLLHQFKTVGVYELKDARGSMHVSNLATDAAARAAL
                     RQKFPADAYLGVQLLGCGAGQQRETERRGTGASAVEVTVYFFRGECTAELTAFDPEGK
                     TIATLQKTGRWDSPRQDRPDGSQMGSQALSLAIEDTARRLAREIRPTAGK"
     gene            complement(2472..>3245)
                     /locus_tag="P5164_18005"
     CDS             complement(2472..>3245)
                     /locus_tag="P5164_18005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRY45830.1"
                     /translation="TGALLANPELVGIGVSGDGDTASIGIGQFVHLVRRNIPLVYVVE
                     NNGVYGLTKGQFSATADVGSTLKGGGANELVPIDLCGLAIELGCGFVARSFSGDMKQL
                     QAILQAAIAHRGTALLDVVSPCVTFNDHEGSTKSYKYAKDHEEPLHEIGFVPYFDDTL
                     AEIPPGETKDVPFPDGSLVRFRSVGRDYDPTDKDQVLATLHRSRTEKEFLTGILYLEP
                     EKAAFAKVLKTVEEPLTALGAERLRPSRETLAAILEEYR"
BASE COUNT          463 a         1125 c         1185 g          472 t
ORIGIN      
        1 tccacgtcgt cgtgaacaac cagatcggct tcacgacgaa cccgaaggac gcccgttcct
       61 cgccgtactg cacggacgtc gccaagatgg tccaggcgcc gatcctccac gtgaacgggg
      121 acgacccgga ggcggtggtc caggccgtcg acctcgcgat ggaataccgg aaggcgttcg
      181 ggcacgacgt cgtcatcgac atggtctgct acaggaggta cggccacaac gaaggggacg
      241 agccgagcta cacccagccg ctcctctacg cgaagatccg gaaccactcc tccgtcgccc
      301 gcctctacgg cgacgtcctc gtccgcgagg gggtcctgac ggcggcccag gtggaggagg
      361 tctgggtcga tgcgaagcag gcgctcgaca gggcttacga cgcctccgcg gcgacgcgga
      421 cccgaggggc cttctccgat tcgctcgtgg cgccggccgc cccccgctac ccggtcgagg
      481 ggagcgtgcg cgaacgcctc acggaggtcg tgcgggccgt ctccacgctc ccgcccgggt
      541 tcgaggtcca cccgaagctg aagccgctgc tgaagaagag ggccgactac gcgagcggcc
      601 gccccgagat cgactgggcg ggcggcgaga tgctcgcgtt cgggctgctc ctccaggaag
      661 ggacgccggt ccgcctctcg gggcaggact ccgggcgcgg gacgttcagc caccgccact
      721 ccgtcctcgc cgacccgagg acgggggccg agtggatccc gctgaacgcg atccgccagc
      781 cgcaggcgcg cttcgaggtc atcgactcct tcctctcgga ggccggcgtc atggggttcg
      841 agttcggcta cgccgtggcg gacccgtcga ccctcgttct ctgggaggcg cagttcggcg
      901 acttcgcgaa tggcgcacag gtcatcatcg accagttcat ctcgggctcc gagcagaagt
      961 ggagccagac ctgcgacctc gtcctcctcc tgccgcacgg ccaggaaggg caggggcccg
     1021 agcactcgtc cgcgcgcctc gagcgtttcc tgacgctctg cgccgaggac aacatgcggg
     1081 tcgcgaacgt gacgactccc gcccagtacc atcacctgct gctgcggcag atgcggggcg
     1141 acgtgaggcg gccgctcgtc gtcatgtcgc cgaagagcct cctgcgtcat ccgaaggccg
     1201 tctcccgttt cgacgagctc gcggagggct cgttccggcc ggtcctcgac gacgcggcgt
     1261 tcgaggcggg cggggcggac ggcgttcgcc gcgtcgtgat cgcgagcggc aagctcgtct
     1321 acgagctgct cgcggcgcga gagaaggccg gccgttccga cgtggcgatc gtgaggctcg
     1381 aggagctcta cccgttcccc ggggaggagc tgggccgcgt gatcgcgcgc tacccgaagg
     1441 acgccgctct cgtcttcgcg caggaagagc ccaagaacat gggcgcctgg cgtttcgtga
     1501 tggagcaggt cctcgacggc gccgtgccgg cggccggagg ccggccgctt cgctacgcgg
     1561 gccggcgccc ctcggccagc ccggctcccg gctcgcatca cgtcttcgcg acggagcagg
     1621 aggcgatcgc ggccgacgcg ctcggcgtcg aggtggaggt cccggcccgc tcctgaccgg
     1681 gccggcggac cgcgggccgc ccggcccgcg gtccggcacg aggcggcgag ggccggcccg
     1741 gacagggccg gccgcgggcc gcctcgggag cccggcgtgc tacttcccgg ccgtcggccg
     1801 gatctcgcgg gcgaggcgcc gcgccgtgtc ctcgatggcg aggctcaggg cctgcgaccc
     1861 catctgcgag ccgtccggcc ggtcctgccg cgggctgtcc cagcggccgg tcttctgcag
     1921 cgtcgcgatc gtcttcccct ccgggtcgaa cgcggtcagc tcggccgtgc attcgccccg
     1981 gaagaagtag accgtcacct ccacggcgct cgccccggtc ccgcgccgct cggtctcccg
     2041 ctgctgcccg gcgccgcacc cgaggagctg gacgcccagg taggcgtcgg cggggaactt
     2101 ctgccgaagg gcggcgcgcg ccgccgcgtc cgtcgcgagg ttcgagacgt gcatcgagcc
     2161 gcgcgcgtcc ttcagctcgt agacgccgac ggtcttgaac tgatggagca gcgagtccgc
     2221 catccgggtc atcggggtcg cctcgcccgt gtcgaggacg acgccgctca tccgcatctc
     2281 ggggtaggtg acctcgacga gcgcgagaga cttgccgggg acgacggtcg cggtggaagc
     2341 gcagcccgcc gcggcgaaga cgaggacgaa aagggcaaag ccgggaagga gaggcagctt
     2401 tcggcgaatc atccgttgat tatgccgcgg ggggcgggcg gaagacagcc gatcgaacgc
     2461 gcgggcccgc ctcaccggta ctcctccagg atcgccgcga gggtctcccg ggaggggcgc
     2521 aggcgctccg cgccgagcgc cgtgagcggc tcctcgaccg tcttcaggac tttcgcgaag
     2581 gccgccttct ccggctcgag gtagaggatg ccggtcagga actccttctc cgtccggctc
     2641 cggtggagcg tcgcgaggac ctggtccttg tcggtcgggt cgtagtcgcg gccgaccgag
     2701 cggaagcgga cgaggctccc gtcggggaac ggaacgtcct tcgtctcgcc gggcgggatc
     2761 tcggcgaggg tgtcgtcgaa gtacgggacg aagccgatct cgtggagcgg ctcctcgtgg
     2821 tccttggcgt acttgtagct cttcgtcgag ccctcgtggt cgttgaacgt gacgcagggg
     2881 gagacgacgt cgaggagcgc cgtgccgcgg tgggcgatcg cggcctggag gatcgcctgg
     2941 agctgcttca tgtcgccgga gaaggagcgg gcgacgaagc cgcagccgag ctcgatcgcg
     3001 aggccgcaga ggtcgatcgg gacgagctcg ttcgcgccgc cccccttgag cgtcgagccg
     3061 acgtcggcgg tcgcggaaaa ctgccccttc gtcaggccgt agacgccgtt gttctcgacg
     3121 acgtagacga gagggatgtt gcgccgcacg aggtggacga actgtccgat cccgatcgag
     3181 gcggtgtcgc cgtcgccgga gacgccaatc ccgaccagct cggggttcgc gaggagcgcg
     3241 cccgt
//
LOCUS       DAOTEZ010000474         3236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_22831_length_3236_cov_5.809180, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000474 DAOTEZ010000000
VERSION     DAOTEZ010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22831_length_3236_cov_5.809180"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /gene="pstC"
                     /locus_tag="P5164_18010"
     CDS             <1..634
                     /gene="pstC"
                     /locus_tag="P5164_18010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRY45831.1"
                     /translation="LIAVPFGLGAAIYLSEFCKPRLRETLKVVIELLSAIPSVVWGFI
                     GLTVMSRLVVSVTGAPVGVNLLNGGILLALMSVPVIVSIGEDALKAVPDSYREAGVAL
                     GATRWQLVWRVLLPAAKNGLLAAVLLGVGRAVGETMAVLMATGHAVNIPGGILEPVRT
                     LTANIAAELGEAPSGSPHYRVLFLTGTLLFLITFVVNLAADFAVRGVRRK"
     gene            637..1530
                     /gene="pstA"
                     /locus_tag="P5164_18015"
     CDS             637..1530
                     /gene="pstA"
                     /locus_tag="P5164_18015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRY45832.1"
                     /translation="MAGRFAKTPHGLRKERTEALAKAVFGLMASAMILPLLLIVGYLV
                     VLAWPALGWEFLVDVPKNGMRDGGIWPAFVGTLYLVGISLVVSTPIGVLAAIYLNEYA
                     KDGWFNRLVNLAVINLAGVPSIVHALFGLGAFVYAAKFGYSILSASLTLAIMTLPVII
                     ASTREALASVPMAFREACWNVGATRWQTIRTVVLPNSISGILTGVILQVSRAAGETAP
                     IMFTGAVFFKAVERGDLFPYGLGEQCMALSMHLYTLATQVPNVKEALPYATAVVLLAT
                     VLLVNATSIAVRIWLRGRKKW"
     gene            1524..2297
                     /locus_tag="P5164_18020"
     CDS             1524..2297
                     /locus_tag="P5164_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HRY45833.1"
                     /translation="MVAGSAAPPEIEVHDLRLSYSGREVIHGISFDLRKNEILAVIGP
                     AQSGKTSMLRCLNRTIDFIPTASVSGTVKVHGEDVRKSKDVFGLRRRIGMVAPLPVGL
                     PLSIYDNVAFAPRCAGVKDRADLDPVVEKCLRQAALWDEVKDRLGSLGTALSGGQQQR
                     LTIARALSHRPEILCLDEFSIAIDPVTTMRIEDVLKELKEEITIVLVTNLTQQARRLS
                     DRTMFLLEGEIVELDDSEVIFSGEPKSPKTRDYVAGVFG"
     gene            2294..3082
                     /locus_tag="P5164_18025"
     CDS             2294..3082
                     /locus_tag="P5164_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HRY45834.1"
                     /translation="MSGVPAAGPDHAIETRGLSLWYGAFQALFDVSADIKKGIITALI
                     GPSGCGKTTLLRCFNRVNERYGNVRTAGEIRVLGESIYAPEVSLVELRKAVGMVFQRP
                     NPLPISVYENVVFGLRVHTPRTELKRSVLDEAVEKALTEVGLWKDLKDRLNAKATTLL
                     LEQQQKLCLARLLPLKPEVILMDEPASALDVGGTRAIEELMFELAGRYTIVIVTHNMA
                     QARRASDECIFMLMGKMVEHARTEDLFLNPTDPRTAEYIEGRYA"
BASE COUNT          483 a         1179 c         1050 g          524 t
ORIGIN      
        1 cctcatcgcc gtcccgttcg gcctcggggc cgcgatctac ctctccgagt tctgcaagcc
       61 gcgcctccgc gagacgctca aggtcgttat cgagctcctc tcggcgatcc cgagcgtcgt
      121 ctgggggttc atcggcctca ccgtcatgag ccggctcgtc gtctcggtga cgggcgcccc
      181 ggtcggggtg aacctcctga acggcgggat cctcctcgcg ctcatgagcg tccccgtgat
      241 cgtctcgatc ggcgaggacg cgctgaaggc ggtccccgac tcgtaccgcg aggcaggcgt
      301 cgccctcggg gcgacgcggt ggcagctcgt ctggcgggtc ctcctgccgg ccgcgaagaa
      361 cggcctcctc gcggccgtcc tcctcggcgt cggccgggcc gtcggcgaga cgatggccgt
      421 cctgatggcg accggccacg cggtgaacat cccgggcggg atcctcgagc ccgtgcggac
      481 gctgaccgcc aacatcgccg ccgagctcgg cgaggccccg tcgggctcgc cccactaccg
      541 cgtcctcttc ctcaccggga cgctcctctt cctcatcacg ttcgtggtca acctcgccgc
      601 cgacttcgcc gtccgcggcg tgcggaggaa atgacggtgg caggccgctt cgcgaagacc
      661 ccgcacggcc tgcggaagga gcggaccgag gcgctcgcga aggccgtctt cggcctgatg
      721 gcctcggcga tgatcctgcc gctcctcctc atcgtcggct acctcgtcgt cctcgcctgg
      781 ccggccctcg ggtgggagtt cctcgtcgac gtcccgaaga acgggatgcg cgacggcggg
      841 atctggcccg ccttcgtcgg gacgctctac ctcgtcggga tctcgctcgt cgtctcgacc
      901 ccgatcgggg tcctcgcggc gatctacctg aacgagtacg cgaaggacgg ctggttcaac
      961 cgcctcgtga acctggccgt catcaacctg gccggcgtcc cgagcatcgt ccacgcgctc
     1021 ttcggcctcg gcgcgttcgt ctacgccgcg aagttcggct actcgatcct ctccgcctcc
     1081 ctaacgctcg cgatcatgac gctccccgtc atcatcgcct cgacgcgcga ggcgctcgcc
     1141 tcggtgccga tggccttccg cgaggcgtgc tggaacgtcg gcgcgacccg ctggcagacg
     1201 atccggaccg tcgtcctccc gaactccatc agcggcatcc tgaccggcgt catcctccag
     1261 gtgagccgcg cggcggggga gacggcgccg atcatgttca ccggcgccgt cttcttcaag
     1321 gcggtcgagc ggggcgacct cttcccgtac ggcctcggcg agcagtgcat ggcgctctcg
     1381 atgcacctct acacgctcgc gacccaggtc ccgaacgtga aggaggcgct cccgtacgcg
     1441 acggcggtcg tcctcctggc gaccgtcctc ctcgtgaacg cgacgtcgat cgccgtccgc
     1501 atctggctga ggggtcggaa gaagtggtag ccgggagcgc cgccccgccc gagatcgagg
     1561 tccacgacct ccggctctcg tactccgggc gcgaggtgat ccacgggatc tccttcgacc
     1621 ttcgaaagaa cgagatcctc gccgtcatcg ggcccgcgca gtcggggaag acgtcgatgc
     1681 tccgctgcct gaaccggacg atcgacttca tcccgacggc ctccgtctcg ggaaccgtga
     1741 aggtccacgg ggaggacgtg aggaagtcga aggacgtctt cggcctgcgc cgccggatcg
     1801 ggatggtcgc cccgctcccg gtcggcctcc ccctgtcgat ctacgacaac gtcgccttcg
     1861 ccccccgctg cgccggcgta aaggaccggg cggacctcga cccggtcgtc gagaagtgcc
     1921 tccgccaggc ggccctctgg gacgaggtga aggaccgcct cgggagcctc gggaccgccc
     1981 tctccggcgg ccagcagcag cgcctgacga tcgcgagggc cctctcccac cgccccgaga
     2041 tcctctgcct cgacgagttc tcgatcgcga tcgacccggt gacgacgatg cggatcgagg
     2101 acgtcctgaa ggagctgaag gaggagatca cgatcgtcct cgtcacgaac ctgacgcagc
     2161 aggcgcgccg cctctcggac cggacgatgt tcctgctgga gggcgagatc gtggagctgg
     2221 acgactccga ggtgatcttc tcgggcgagc cgaagagccc gaagacgcgc gactacgtcg
     2281 ccggggtgtt cggatgagcg gcgtccccgc ggccgggccc gaccacgcga tcgagacccg
     2341 cgggctctcg ctctggtacg gcgccttcca ggcgctcttc gacgtgtcgg ccgacatcaa
     2401 gaaggggatc atcaccgccc tcatcgggcc gagcggctgc gggaagacga cgctcctccg
     2461 ctgcttcaac cgggtcaacg agcgctacgg caacgtccgg acggccggcg agatccgggt
     2521 cctcggcgag agcatctacg cgcctgaggt ctcgctcgtc gagctccgca aagccgtcgg
     2581 gatggtcttc cagcggccga acccgctccc gatctccgtc tacgagaacg tcgtcttcgg
     2641 cctgcgcgtc cacacgccgc ggaccgagct gaagcggagc gtgctcgacg aggcggtcga
     2701 gaaggcgctc accgaggtcg gcctctggaa ggatctgaag gaccgcctga acgcgaaggc
     2761 gacgacgctc ctcctcgagc agcagcagaa gctctgcctc gcccgcctcc tcccgctcaa
     2821 gcccgaggtc atcctgatgg acgagcccgc ctccgcgctc gacgtcggcg ggacgcgcgc
     2881 catcgaggag ctgatgttcg agctggccgg gcgctacacc atcgtcatcg tgacgcacaa
     2941 catggcgcag gcgcggcggg cgagcgacga gtgcatcttc atgctcatgg ggaagatggt
     3001 cgagcacgcc cgcaccgaag acctcttcct gaacccgacg gacccgagga ccgcggagta
     3061 catcgaaggg cgctacgcgt gagcggggtc cgcgcggcac tttcgctttc cgtacgctcc
     3121 gggatcggta gactgcctcg accatgtgcc agtcggaccg cgctgcgacg aactccattg
     3181 ccggatcccg attccctctc acacaataca ttattcgaga cctgacccca tccctg
//
LOCUS       DAOTEZ010000475         3229 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_22898_length_3229_cov_6.427851, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000475 DAOTEZ010000000
VERSION     DAOTEZ010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22898_length_3229_cov_6.427851"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="P5164_18030"
     CDS             <1..227
                     /locus_tag="P5164_18030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRY45835.1"
                     /translation="GFKVQSIPGIGPWLDANLPTFITQGLGVPFYFGGTSLLIVVGVA
                     MDTVQQIESQLVMRNYDGFLKKGRIRGRRS"
     gene            232..822
                     /locus_tag="P5164_18035"
     CDS             232..822
                     /locus_tag="P5164_18035"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF011105.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRY45836.1"
                     /translation="MPVDVVFFGPPGSGKGTQASRLASTLGIPQVSTGDLLRSNVARG
                     TDLGKVARPIMESGALVPDDLVTRMLKERLEEPDAATGALFDGYPRTVPQAEELDRLL
                     RIEGRTLTAVLFIDVPDPVIVGRLVKRAEIEGRADDTPETVAARLRVYRDKTEPLAEL
                     YRKLGLFHRIDGDRPVDEVAFDVLATLNRVTGRVLA"
     gene            819..1679
                     /gene="map"
                     /locus_tag="P5164_18040"
     CDS             819..1679
                     /gene="map"
                     /locus_tag="P5164_18040"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRY45837.1"
                     /translation="MITCKGRAELLKMERATWIVQQTLADCVAACRPGVTTEEIDRIA
                     ADGIRKRGGKAAFPGYRGYPKTVCSSVNDEVVHGIPTSKRKLRDGDIVGIDLGVIVDG
                     YFGDAARTAVVGEVPAPVAELVRQTWLALVAGVSAVRVGGRIGDIGAAVEAVANEKGF
                     GVVREYVGHGIGTALHEEPQVPNYGPAGKGERIREGMTLAIEPMFNLGTARVRLGPDG
                     WTVRTADGKPSAHFEHTVVATADGPVVLGFGRYTSDGLVTGVPDETEYPVPAASPRGE
                     PAETPGESRV"
     gene            1703..1921
                     /gene="infA"
                     /locus_tag="P5164_18045"
     CDS             1703..1921
                     /gene="infA"
                     /locus_tag="P5164_18045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRY45838.1"
                     /translation="MSKEDAIEVTATVIEPLPNAMFKVELENKHQVLAHISGKMRKHF
                     IRILPGDKVLVELSPYDLNRGRIIYRYK"
     gene            1983..2096
                     /gene="rpmJ"
                     /locus_tag="P5164_18050"
     CDS             1983..2096
                     /gene="rpmJ"
                     /locus_tag="P5164_18050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRY45839.1"
                     /translation="MKVRASVKKICSKCKVIRRAGVVRVICENAKHKQRQG"
     gene            2109..2492
                     /gene="rpsM"
                     /locus_tag="P5164_18055"
     CDS             2109..2492
                     /gene="rpsM"
                     /locus_tag="P5164_18055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRY45840.1"
                     /translation="MARISGVDLPPNKRVEIGLTYIFGIGRPTARMILKDTGVGPDTR
                     IKDLSEDEVAKIRKKIQDDVKVEGDLRKETAQDIKRLMDIGCYRGLRHRRGLPVRGQR
                     THTNARTRKGPRRGAVAGKKKATKK"
     gene            2508..2909
                     /gene="rpsK"
                     /locus_tag="P5164_18060"
     CDS             2508..2909
                     /gene="rpsK"
                     /locus_tag="P5164_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRY45841.1"
                     /translation="MAAAEAKGRKKGPKKEKKNVPHAVATVQASFNNTIVAITDPSGN
                     VLAWSSAGRIGFKGSRKGTPFAAQLAAGNAAQLAQEHGVRTLDVRVQGPGAGRESAIR
                     ALAAAGLDIRSIKDATPIPHNGCRPRKRRRV"
     gene            3111..>3229
                     /locus_tag="P5164_18065"
     CDS             3111..>3229
                     /locus_tag="P5164_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRY45842.1"
                     /translation="MARYRESVCRLCRREAMKLFLKGDRCFTNKCAIERRNFPP"
BASE COUNT          555 a         1094 c         1161 g          419 t
ORIGIN      
        1 tgggcttcaa ggtccagagc atcccgggaa tcggaccgtg gctcgacgcg aacctcccga
       61 cgttcatcac ccagggcctc ggcgtgccgt tctacttcgg agggacgtcg ctcctgatcg
      121 tcgtcggcgt cgcgatggac accgtgcagc agatcgagtc gcagctcgtg atgcggaact
      181 acgacgggtt cctgaagaag ggccgcatcc gggggcggcg gagctaggac gatgccggtc
      241 gacgtggtgt tcttcggccc gccgggctcg ggaaagggga cccaggcctc gcgcctggcg
      301 tccacgctcg ggattcccca ggtgtccacc ggagacctcc tccgctcgaa cgtggcccga
      361 ggcaccgacc tcgggaaggt cgcccggccg atcatggagt cgggggcgct cgtccccgac
      421 gacctcgtca cacgcatgct gaaggagagg ctcgaggagc ccgacgcggc gaccggcgcc
      481 ctcttcgacg gctatccgcg caccgtgccg caggccgagg agctcgaccg gcttctccgg
      541 atcgagggcc gcaccctgac ggcggtgctc ttcatcgacg ttcccgaccc cgtcatcgtc
      601 ggcaggctcg tgaagcgggc cgagatcgag ggacgggcgg acgacacgcc ggagaccgtc
      661 gcggcgcgcc tccgggtcta ccgcgacaag accgagccgc tcgccgagct ctaccggaag
      721 ctcgggctct tccaccggat cgacggcgac cggcccgtcg acgaggtcgc gttcgacgtc
      781 ctggcgacgc tgaaccgcgt caccgggagg gtcctggcgt gatcacctgc aagggccggg
      841 cggagctcct gaagatggag cgggcgacgt ggatcgtcca gcagacgctg gccgactgcg
      901 tcgccgcctg ccgacccggc gtgacgacgg aggagatcga ccggatcgcc gcggacggca
      961 tccggaagag gggcggaaag gccgccttcc cgggctaccg cggctacccg aagaccgtct
     1021 gctcgtcggt gaacgacgag gtcgtccacg ggatcccgac gtcgaagcgg aagctccgcg
     1081 acggggacat cgtcgggatc gacctcggag tcatcgtcga cggctacttc ggcgacgcgg
     1141 cgcggacggc ggtcgtgggc gaggtgcccg cgccggtcgc ggagctcgtc cgccagacgt
     1201 ggctcgctct cgtggccggc gtctcggcgg tccgcgtcgg cggccgcatc ggcgacatcg
     1261 gcgcggcggt cgaggccgtc gcgaacgaga agggcttcgg cgtcgttcgc gaatacgtcg
     1321 ggcacggcat cgggacggcc ctccacgaag agccccaggt gccgaactac gggccggcag
     1381 ggaagggcga gcggatccgc gaggggatga ccctcgcgat cgagccgatg ttcaacctcg
     1441 ggacggcccg ggtccggctc gggcccgacg gctggaccgt gaggactgcc gacggcaagc
     1501 cgtcggcgca cttcgagcac acggtcgtgg cgaccgccga cgggccggtc gtcctcggct
     1561 tcggccgcta cacgtccgac ggtctcgtga cgggggtccc ggacgagacg gaatacccgg
     1621 tccccgcggc ttcgccgcgg ggggagccgg ccgagacccc gggcgagagc cgggtctgag
     1681 aggagagggt ttcgaggcgc agatgtcgaa ggaagacgcc atcgaggtga ccgcgacggt
     1741 catcgagccc ctgccgaacg cgatgttcaa ggtggagctc gagaacaagc accaggtcct
     1801 ggctcacatc tccgggaaga tgcggaagca cttcatccgc atcctgcccg gggacaaggt
     1861 cctcgtggag ctctctccgt acgacctgaa ccggggtcgc atcatctacc ggtacaagta
     1921 gtaaactgta aggctccgcc ggctcccccg ggaggccggg ggcgaaacga tcgagggatc
     1981 acatgaaagt ccgcgcgtcc gtgaagaaga tctgctccaa gtgcaaggtg atccgcaggg
     2041 cgggggtcgt ccgggtcatc tgcgagaacg ccaagcacaa gcagcggcag ggttagaggg
     2101 aagagggaat ggctcgaatc tccggcgtcg atctcccccc gaacaagcgt gtcgagatcg
     2161 gtctcaccta catcttcggg atcgggcgcc ctacggcccg catgatcctg aaggacacgg
     2221 gcgtgggacc cgacacgcgg atcaaggacc tgtccgagga cgaggtcgcg aagatccgca
     2281 agaagatcca ggacgacgtg aaggtcgagg gcgacctccg caaggagacc gcccaggaca
     2341 tcaagcgtct gatggacatc ggctgctacc gcgggcttcg tcaccgccgc ggcctgccgg
     2401 tccgtgggca gcggacgcac acgaacgccc gcacccggaa gggcccgcgt cgcggcgccg
     2461 tggccggcaa gaagaaggcg acgaagaagt aaggatgagg aacgccgatg gctgccgctg
     2521 aagctaaggg ccggaagaag ggccccaaga aggaaaagaa gaacgtcccg cacgccgtgg
     2581 ccacggtcca ggcgtcgttc aacaacacga tcgtggcgat caccgacccg tcggggaacg
     2641 tcctcgcctg gtcgtccgcc ggccggatcg gcttcaaggg ctcccggaag gggacgccgt
     2701 tcgcggcgca gctcgccgcc ggcaacgccg cgcagctcgc gcaggagcat ggcgtgagga
     2761 cgctcgacgt ccgcgtccag ggtcccggcg ccggccgcga gagcgcgatc cgcgccctcg
     2821 ccgcggccgg gctcgacatc cgatccatca aggacgcgac cccgatcccg cacaacggct
     2881 gccgcccccg caagcggcgc cgggtctagt ccctcacccc gttccggggg agcgccgctc
     2941 ccccggcggg tcggcgagag agggaggccc gcgggcctcc cgacccccct ccgagggggg
     3001 cgactccggc gcgcggagag cgcgccggcg cccgtaaacc ggctcccgat tccgggccgg
     3061 gacgaagggc ggaacgcaac gacggagtcg gccaggaggt atccgttccc gtggcgcgtt
     3121 atcgcgaatc cgtctgccgg ctctgccggc gcgaggccat gaagctcttc ctgaagggcg
     3181 accgctgctt cacgaacaag tgcgccatcg agcgccgcaa cttcccgcc
//
LOCUS       DAOTEZ010000476         3227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_22921_length_3227_cov_5.598676, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000476 DAOTEZ010000000
VERSION     DAOTEZ010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22921_length_3227_cov_5.598676"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2398
                     /gene="drmD"
                     /locus_tag="P5164_18070"
     CDS             <1..2398
                     /gene="drmD"
                     /locus_tag="P5164_18070"
                     /inference="COORDINATES: protein motif:HMM:NF038317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DISARM system SNF2-like helicase DrmD"
                     /protein_id="HRY45843.1"
                     /translation="SATPHNGHSNSFSSLLELLDPLRFCRGVPVKGPKLLDAVMVRRL
                     KGDLREIVGDLPRRDVVQVDVDGLPPDAPELRLAELLDRYATLRAERLSGARKTAQAA
                     AKLVTTHLQKRLFSSVEAFARTLKVHRASLEKAEGKARRDDEIALASLTLLAEPPSAD
                     DDRAELSEEELSAATDAEVDRATRAALPPAGAALVSAEERALLDEMAAIAEEARYVPG
                     PRAKLLVEWIRANLLDSSSGAPAWNRRRLLVFTEFTDTMRYLAGQLRSALAAIDRDGA
                     AEVRIEQRIAVFHGGLGDETREELKAAFNAEPADHPLRILIATDSAREGVNLQNHCAD
                     LFHFDVPWNPGRMEQRNGRIDRQLQREKSVRCHYFFFRQRPEDKVLKTLVEKTETIAK
                     ELGSLSAVLEKRLGALLEGGIRRSEADALARRLELESPTDRERLAAEELEEARERKEA
                     LAAQLDDLRGVMEASRRALGFDEDAFREALSSSLELGKAPPLALTSVKGRPAYRFPAL
                     DQRAGADPTWATTLDTLRRRRKKDERPWDWRREAPPRPVVFADPGTLDDEFVHLHLEH
                     RVARRLLGRFLSQGFVHDDLSRACCAQTRDPVPRVVLLGRLSAWGDGAARLHDEVIAL
                     AARWVEPDARKGPLRPYAETAEERALALLEESLLASAGTPVPDSTRSRLLATSARDLE
                     DLLPALRERGEETLRRVAERLADRGRREADELRAVLLAQKARIEAERAKHADPQLELG
                     FADDEKRQLAADRRHQERRLAELGRELTSEPDRIRRSFEVRATRLEPVGLAYLWPVSG
                     "
     gene            2413..>3227
                     /locus_tag="P5164_18075"
     CDS             2413..>3227
                     /locus_tag="P5164_18075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HRY45844.1"
                     /translation="MASDAALRAHQEWLGYIQPVGLVVSPAALSAAGCVPDRNVAAQQ
                     ETLRGLLSGDPPRLLDFPAFTREFLGWQEGDLAGAPGGPPLPESLFVALPNEHDVLRP
                     TYAVPDPDSVGRFLLLVSVVPPGADLDDAGARNERGPADGWHASPQARLERLLRETGV
                     PAGLLVNGDALRLVAAPKGESAGWLHFPLASLCEVAGRPLCSALLMLLSAERLFTLPT
                     NERLPALLRESRKYQNLVSTRLAGQVLDALHELVRGFQAAHEASGGALLADVL"
BASE COUNT          405 a         1405 c          997 g          420 t
ORIGIN      
        1 ctcggccacc ccccacaacg gtcactcgaa cagcttctcc tcgctcctcg agctcctcga
       61 cccgctccgc ttctgccgcg gcgtcccggt gaaggggccg aagctcctcg acgccgtcat
      121 ggtgcggcgc ctgaagggcg acctccgcga gatcgtcggt gacctcccgc ggcgtgacgt
      181 cgtccaggtc gacgtcgacg gcctcccgcc cgacgccccc gagctgcgcc tcgccgagct
      241 cctcgaccgc tacgccacgc ttcgggccga gcgcctctcc ggagcgcgaa agacggccca
      301 ggccgcggcg aagctcgtca cgacgcacct ccagaagcgg cttttctcct ccgtcgaggc
      361 cttcgcccgc acgctcaagg tccaccgcgc ctcgctcgag aaggccgagg gaaaggcccg
      421 ccgcgacgac gagatcgccc tcgcctcgct cacgctcctc gcagagcctc cctcggccga
      481 cgacgaccgg gccgagcttt ccgaggagga gctttccgct gccaccgacg ccgaggtcga
      541 ccgcgccacg cgcgcggccc tcccgcccgc gggtgcggcc ctcgtctccg ccgaggagcg
      601 cgccctcctc gacgagatgg ccgccatcgc cgaggaggcc cgctacgtcc ccggcccgcg
      661 cgcgaagctc ctcgtcgagt ggattcgcgc gaacctcctc gactcctcct ccggcgctcc
      721 cgcctggaac cggcgccgcc tcctcgtctt caccgagttc accgacacga tgcggtacct
      781 cgccgggcag ctccgctcgg ccctcgccgc catcgaccgc gacggcgccg ccgaggtccg
      841 catcgagcag cgcatcgccg ttttccacgg cggcctcggc gacgagacgc gcgaggagct
      901 gaaggccgcc ttcaacgccg aaccggccga ccacccgctc cgcatcctca tcgcgaccga
      961 ctcggcccgc gagggcgtca acctccagaa ccactgcgcc gacctcttcc acttcgacgt
     1021 cccctggaac cccggccgca tggagcagag gaacggccgc atcgaccgcc agctccagcg
     1081 cgagaagagc gtccgctgcc actacttctt cttccggcag cggccggaag acaaggtcct
     1141 gaagaccctc gtcgagaaga ccgagacgat cgcgaaggag ctggggagcc tctcggccgt
     1201 cctcgagaag cggctcggcg ccctcctcga ggggggcatc cgccgctccg aggccgacgc
     1261 cctcgcgcgc cgtctcgaat tggagtcgcc caccgaccgc gagcggctcg ccgccgaaga
     1321 gctcgaggaa gcgcgcgagc gaaaggaagc cctcgccgcc cagctcgacg accttcgcgg
     1381 cgtgatggag gcctcccgcc gcgccctcgg cttcgacgag gacgccttcc gcgaggccct
     1441 ctcctcctcg ctcgagctgg ggaaggcccc gccgctcgcg ctcacgtccg tcaagggccg
     1501 ccccgcgtac cgcttcccgg ccctcgacca gcgggccggc gcggacccca cctgggccac
     1561 gacgctcgac acgcttcgcc gccgccggaa gaaggacgag aggccgtggg actggcgccg
     1621 cgaggccccg ccgcgccccg tcgtcttcgc cgaccccggc accctcgacg acgagttcgt
     1681 ccacctccac ctcgagcacc gcgtcgcccg ccgcctcctc ggccgctttc tctcgcaggg
     1741 cttcgtccac gacgacctct cccgcgcctg ctgcgcgcag acgcgcgacc cggtgccccg
     1801 cgtcgtcctc ctcggccggc tctcggcctg gggcgacggc gccgcgcgcc tccacgacga
     1861 ggtcatcgcc ctcgccgccc gctgggtcga gcccgacgcc cgcaagggcc ctctccgccc
     1921 ctacgccgag accgccgagg agcgcgccct cgccctcctc gaggagtcgc tcctcgcctc
     1981 ggccggcacc cccgtccccg actccacgcg ttcccgtctc ctcgccacct cggcccgcga
     2041 cctcgaagac cttctccccg cgctccgcga aaggggcgag gagacccttc gccgcgtcgc
     2101 cgagcgcctc gccgaccgcg gccggcgcga ggccgacgag ctgcgcgccg tcctccttgc
     2161 gcagaaggcc cgcatcgagg ccgagcgcgc gaagcacgcc gacccgcagc tcgagctcgg
     2221 gttcgccgac gacgagaagc gccagcttgc cgccgaccgc cgccaccagg agcgccgcct
     2281 cgccgagctg ggacgggagc tgacctccga gcccgaccgc atccgtcgtt ccttcgaggt
     2341 ccgcgccacg cgcctcgagc ccgtgggcct cgcctacctc tggccggtgt cgggataggg
     2401 aggggagcga cgatggcttc cgacgccgcc ctccgcgccc atcaggagtg gctcggctac
     2461 atccagccgg tcgggctggt cgtctcgccg gccgccctct ccgccgccgg gtgcgtcccc
     2521 gaccgcaacg tcgccgcgca gcaggagacc ctccgcggcc tcctctcggg cgatccgccc
     2581 cgcctcctcg atttcccggc cttcacgcgc gaattcctcg gctggcagga gggcgacctc
     2641 gccggcgcgc ccggcggccc gccgctcccc gagtcgcttt tcgtcgccct cccgaacgag
     2701 cacgacgtcc ttcgccccac gtacgccgtc cccgaccccg actccgtcgg ccgcttcctc
     2761 ctcctcgtct ccgtcgtccc gcccggcgcc gacctcgacg acgccggggc gcggaacgag
     2821 cgcggcccgg ccgacggctg gcacgcgagc ccccaggcgc gcctcgagcg cctcctccgc
     2881 gagacgggcg tccccgcggg ccttctcgtc aatggcgacg ccctccgcct cgtcgccgcg
     2941 ccgaaggggg agtcggccgg ctggctccac ttcccgctcg cgagcctctg cgaggtggcg
     3001 gggcgccccc tctgctcggc cctcctcatg ctcctctcgg ccgagcggct cttcacgctc
     3061 cccacgaacg agcgcctccc ggccctcctc cgcgagagcc gcaagtacca gaacctcgtc
     3121 tccacccgcc tcgccggcca ggtgctcgac gccctccacg agctcgtccg cggcttccag
     3181 gcggcccacg aggcctccgg cggcgccctc ctcgccgacg tcctcga
//
LOCUS       DAOTEZ010000477         3221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_22986_length_3221_cov_5.134239, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000477 DAOTEZ010000000
VERSION     DAOTEZ010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22986_length_3221_cov_5.134239"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3221
                     /locus_tag="P5164_18080"
     CDS             <1..>3221
                     /locus_tag="P5164_18080"
                     /inference="COORDINATES: protein motif:HMM:NF019927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase subunit L"
                     /protein_id="HRY45845.1"
                     /translation="NARLQVEHTVTEVTTGLDLVKLQIHVARGGRLVGDPPAPSGHAI
                     EVRLNAEDPDAGFAPSPGKFLHFRLPTGPGLRIDRGVTEGDTVAPEFDPMIAKLIARG
                     ADRAEALARLRRALSESAVVLDGGTTNKAFLLELLDRPEVRSARVDTGWLERWTAERA
                     GEERRHAAIALLAAAVEAYDDELRVEEEKFYQSAARMRPETRKEVGRTIEFRHRGANY
                     KLRAFRLDRHDYRVELDGKKCEVRVEPLSPYERWLHVGGVRYRVLCVVQGLEHLVEVD
                     GVPHRFSRDDLGILRAPSPAVVVSLGVAAGDRVSRGQRIATLEAMKMEMPIPAPFDGV
                     VRTVFVVPNVQVGPGAPLVQLDPAAQADETSPTASRIALPNGPAAPVAGSLRPLAILD
                     EIRRLVLGFDVDPADARRMTREYVDACPALATDPECLEAEERILRLFADVSGLFRRQT
                     GSEDPDAEGVVSTGEYLLTYLRTYDTQTPDLPPRFVDRLSRAAGHYGVEGTRKGPALR
                     EALLWIWKSRQRAEHQVEPLMAILERRLAAASSAPAGGSPELRSLLDRLVAVTEGRWP
                     ALGDVARDLRYRVFDQPAFEAARRAVYAEMERVFESLAADPSAPGREDGVRRLVECPQ
                     PLQSLYMSRFEGASPAVQGLMLEVLTRRYYRIRTLEGFREVSFGGRAGALAEYAFEGV
                     RHLVVTTWCPDDELDAALGDVAAYLAAHPSEHDVLVDVYSWRPGPLGDADAKAAEVKR
                     VADAAGLGPRVVRVVLALSAAGAGLGMGGMQHFTFRPGDGGFVEEKLYRAMHPMIGKR
                     LDLWRLEGFDIERLPSAEDVYLFKGVARENPKDERLFALVEVRDLTPAFDAQGRFVGL
                     PQMERMLLEAFEAIRHVQAHRRPEDRLQWNRVFVYVWPPFTYGATVVRAFAKHLAPAA
                     EGLGMEKVAIQARIPDPAIPGGLKEVVFSVSQPGRTGITVSVTPPSRKPLRTLSEYEQ
                     KVVRLRQRGLTYPYEILRMLTPPKDDAHADFPPGDFVEHDLDEAGALVPVSRPPGKNR
                     ANIVAGLLTSYTAKVPEGMTRVVLLGDPMGAMGSLAEPECRRIL"
BASE COUNT          418 a         1268 c         1095 g          440 t
ORIGIN      
        1 aacgcgcgcc tgcaggtcga gcacaccgtc accgaggtga cgaccggcct cgacctcgtc
       61 aagctccaga tccacgtggc gcgcgggggc cgcctcgtcg gcgacccgcc cgccccgtcg
      121 ggccacgcca tcgaggtccg cctcaacgcc gaggacccgg acgccgggtt cgccccgtcg
      181 ccggggaagt tcctccactt ccggctgccg accgggccgg gcctccgaat cgaccgcggg
      241 gtgaccgagg gcgacacggt ggcgccggag ttcgacccga tgatcgcgaa gctgatcgcg
      301 cgcggagccg accgggccga ggcgctcgcg cgcctccgcc gcgcgctctc ggagagcgcc
      361 gtcgtcctcg acggggggac gacgaacaag gcgttcctcc tcgagctcct cgaccgtccc
      421 gaggtccgga gcgcgcgggt cgacaccggc tggctcgagc gctggacggc cgagcgggcc
      481 ggggaggagc gtcgccatgc cgcgatcgcg ctcctcgccg cggccgtcga ggcctacgac
      541 gacgagctcc gggtcgagga ggagaagttc taccagtcgg ccgcgcgcat gcgccccgag
      601 acccgcaagg aggtcgggcg gacgatcgag ttccggcacc gcggggcgaa ctacaagctc
      661 cgcgccttcc gcctcgaccg tcacgactac cgcgtggagc tggacggcaa gaagtgcgag
      721 gtgcgcgtcg agccgctctc gccctatgag cgctggctcc acgtcggcgg cgtccgctac
      781 cgcgtcctct gcgtcgtgca ggggctggag cacctcgtcg aggtcgacgg cgttccccat
      841 cgcttctcgc gcgacgacct cggcatcctc cgcgcgccct ccccggccgt cgtcgtcagc
      901 ctcggcgtcg cggcggggga ccgcgtctcc cgcgggcagc ggatcgccac gctcgaggcg
      961 atgaagatgg agatgccgat cccggcgccg ttcgacggcg tcgtccggac ggtcttcgtc
     1021 gtcccgaacg tccaggtcgg ccccggcgcc cctctcgtcc agctcgatcc ggccgctcag
     1081 gcggacgaaa cctcgcccac ggcgtcccgc atcgccctcc cgaacgggcc ggccgcgccg
     1141 gtcgccggtt cgctcaggcc cctcgccatc ctcgacgaga tccgcaggct cgtcctcggc
     1201 ttcgacgtcg accccgccga cgcgcggagg atgacgcgcg aatacgtgga cgcgtgtccg
     1261 gccctggcca ccgacccgga gtgcctcgag gcggaagaac ggatcctccg cctcttcgcc
     1321 gacgtctccg gcctcttccg ccggcagacc ggctccgagg accccgacgc cgagggcgtc
     1381 gtctcgacgg gcgagtacct cctcacctac ctccggacct acgacaccca gacccccgac
     1441 ctgccgcccc gcttcgtcga ccggctctcc cgggccgccg ggcactacgg cgtggaaggg
     1501 acgcgcaagg gccccgcgct ccgcgaagcg ctcctctgga tctggaagtc ccgccagcgg
     1561 gccgagcacc aggtcgagcc gttgatggcg atcctcgagc gccgcctcgc cgcggcctcc
     1621 tcggcgccgg ccggcggctc gccggagctc cgctcgctcc tcgaccgtct cgtcgccgtg
     1681 accgaggggc gctggccggc gctcggcgac gtggcgcgcg acctgcgtta ccgcgtcttc
     1741 gaccagccgg ccttcgaggc ggcgcgccgc gcggtctacg ccgagatgga gcgcgtcttc
     1801 gagagcctcg ccgccgaccc ctccgcgccc gggcgcgagg acggcgtccg ccggctcgtg
     1861 gagtgccctc agcctctcca gagcctctac atgagccgct tcgagggggc ttcgccggcc
     1921 gtccaggggc tcatgctcga ggtcctgacc cgccgctact accggatccg gacgctcgaa
     1981 ggtttccgcg aggtctcctt cgggggccgc gccggggccc tcgccgagta cgcgttcgaa
     2041 ggggtccgcc acctcgtcgt cacgacgtgg tgcccggacg acgagctcga cgccgccctc
     2101 ggcgacgttg ccgcctacct cgcggcgcac ccgtcggagc acgacgtcct cgtcgacgtc
     2161 tattcctggc gtcccggccc gctcggcgac gccgacgcga aggccgccga ggtcaagcgc
     2221 gtggccgacg cggccggcct cggcccacgc gtcgtccggg tcgtcctcgc cctctcggcg
     2281 gccggcgccg ggctcggcat gggcgggatg cagcacttca ccttccggcc cggcgacggc
     2341 ggcttcgtcg aggagaagct ctaccgggcg atgcacccga tgatcggcaa gcgcctcgac
     2401 ctctggcgac tcgagggctt cgacatcgag cggctcccgt cggcggagga cgtctacctc
     2461 ttcaagggcg tcgcccgcga gaacccgaag gacgagcgcc tcttcgccct cgtcgaggtg
     2521 cgcgacctga cacccgcctt cgacgcgcag gggcgcttcg tcggccttcc ccagatggag
     2581 cggatgctcc tcgaggcgtt cgaggcgatc cgccacgtcc aggctcaccg gcggcccgag
     2641 gaccggctcc agtggaaccg ggtcttcgtc tacgtctggc cccccttcac ctacggcgcg
     2701 acggtcgtcc gggccttcgc caagcacctc gcgccggcgg ccgaggggct cggcatggag
     2761 aaggtcgcca tccaggcgcg catccccgac cccgcgattc ccggcggcct caaggaggtc
     2821 gtcttctcgg tctcgcagcc gggccggacc ggaatcaccg tctccgtcac gccgccgagc
     2881 cgcaagccgc tccggaccct ttccgagtac gagcagaagg tcgtgcgcct ccgccagcgc
     2941 ggcctcacct acccttacga gatcctccgg atgctgacgc ccccgaagga cgacgcgcac
     3001 gccgacttcc cgccgggcga cttcgtcgag cacgacctcg acgaagcggg cgccctcgtc
     3061 cccgtgagcc ggccgcccgg gaagaaccgg gcgaacatcg tggccggcct cctgacgagc
     3121 tacacggcga aagtcccgga gggaatgacg cgcgtcgtcc tcctcggcga cccgatgggg
     3181 gcgatgggct ccctcgccga gcccgagtgc cgccgcatcc t
//
LOCUS       DAOTEZ010000478         3219 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_23008_length_3219_cov_4.298040, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000478 DAOTEZ010000000
VERSION     DAOTEZ010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23008_length_3219_cov_4.298040"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..163)
                     /locus_tag="P5164_18085"
     CDS             complement(<1..163)
                     /locus_tag="P5164_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRY45846.1"
                     /translation="MTDPKHALAAEIARRRTFAIISHPDAGKTTLTEKLLLYGGAIHL
                     AGSVKSRKAA"
     gene            284..>3219
                     /locus_tag="P5164_18090"
     CDS             284..>3219
                     /locus_tag="P5164_18090"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45847.1"
                     /translation="MRGLSAKLIASSVCMMFILGATLRSGGARAEGSRSLYPSSYPAA
                     GFRANLDLQPGPANKYVSRVMRRGFLYVYAQAGEYIVLGSRNRSNGGNISVYDPQSFG
                     TPGDETIPGTASFTCSGGSAAPGPHYFGGTRGTISTRAQELAGPNSADNSATVTNGFQ
                     PCAYRAPSTGIYGVLFAAATSGGSGPDGSIATPNVGNNSVSAWDVTVRANATSTVDLN
                     GRLFTYAFVGFTGGNSRPVYSTHYYVTADGYRYKEDLSGPMNLGLDPNGYAVYANALG
                     FLDNLQPLYKDIRGADALVTTNVPPGVTTQIAQYPLFFSDVSPGANDTELSRVLGALG
                     IPVTPPSPTVANVSFSGHVGGSTTTTGAGGTFTFTTTDTITFQIVVSRDGVDFSTATL
                     ANAVVTGVAGTGTHTITWDGKDQGMTNFPSSATPYSFRVYGRAGEIHFPIIDAEGNPG
                     AGPIVTRLNGTGAPSTTVFFDDRGYVTSSGTTVGVLNGTLCNATPPTPPSPQFALDGV
                     DSSSTAGGGYRDWLGNGNSNSDCAATAGWGDAKALNIWTYYSAPFETKTLVVDPIIID
                     VATTVSAPASAVAGSTVQGAFSFGNNGNSTALGVTYGMTLTPGLGTVTFGNLPGGVTA
                     TYNNVTGAVTFTGLPTSLTPGQAISGMTFSYTAPASGPVVATTSVGTTSTDSYLPNNT
                     ATVSTGIGAVDVLTTVSVPATATTGSTVSGSFQFANFGANAAGSVTYSATIGAPGNYP
                     ASVSFTALPFGVTATYNPVNGQVTFTGMPTTLASGQQLGFGFSYPAPGPGTVPVATTI
                     TTASSDANPLNNAAADATTIAAPDLTITKSDGGATFTVGLNGTYTLTVTNTGTAETTG
                     DITVTDTLTAGLTFVSGMGPSFSCSAALQVVTCTRLAANPIAIGTSVPITLVVSVGPA
                     ALPGVTNSATVSTPNESNTGNNTGSDTTPVAATAHLSITKTDGVATVVPGSSVTYTIV
                     ASNAGPS"
BASE COUNT          516 a         1177 c         1055 g          471 t
ORIGIN      
        1 gcgccgcctt gcgcgacttc accgagccgg cgaggtggat cgccccgccg tagagcagca
       61 gcttctccgt cagcgtcgtc ttgccggcgt cgggatggct gatgatcgcg aaagtccggc
      121 ggcgggcgat ctcggccgcg agggcgtgct tcgggtcggt catggctcgg gccgccgcga
      181 gggccgtggg gccgggcggc ggggggcgga ctgtagcatc gggtgcgccg ggccgtcgtg
      241 agagaatttc cgtttgggcc tggcgaggcc tgcgaggtgt ggagtgagag gtctttctgc
      301 gaagctcatc gcttcgtccg tctgcatgat gttcatcctc ggcgcaaccc tcaggtccgg
      361 cggggcgcgg gccgagggga gtcgttccct gtacccgtcg agctacccgg cggcggggtt
      421 ccgcgcgaac ctggatctcc agcccggccc ggcgaacaag tacgtcagcc gggtcatgcg
      481 gcgcggcttc ctctacgtct acgcccaggc gggtgagtac atcgtcctcg gctcgaggaa
      541 caggtcaaac ggcgggaaca tctcggtcta cgacccccag agcttcggga cccccggcga
      601 cgagacgatt cccggcacgg cgagcttcac ctgctccggg ggctcggcgg ctcccggccc
      661 ccactacttc ggcgggacgc gcgggaccat ctcgacccgg gctcaggagc tggccggccc
      721 gaacagcgcg gacaactcgg cgaccgtcac gaacggattc cagccgtgcg cctaccgcgc
      781 cccctcgacc gggatctacg gcgtcctctt cgcggccgcg acgtccggcg gctcgggtcc
      841 ggacgggtcg atcgcgacgc cgaacgtcgg caacaacagc gtctcggcct gggacgtgac
      901 cgtccgggcg aacgcgacgt cgacggtgga cctgaacggc cgcctcttca cgtacgcctt
      961 cgtcgggttc acgggaggca actcccggcc ggtctactcg acgcactact acgtgacggc
     1021 cgacggctac cgctacaagg aagacctctc tggaccgatg aacctcggtc tcgacccgaa
     1081 cgggtacgcc gtctacgcga acgcgctcgg cttcctcgat aacctccagc ccctctacaa
     1141 agacatccgg ggcgccgacg ccctcgtgac gacgaacgtg ccgcccggcg tgacgacgca
     1201 aatcgcccag tacccgctct tcttcagcga cgtctcgccc ggcgcgaacg acaccgagct
     1261 ctcccgcgtc ctcggcgcgc tcgggatccc ggttactccc ccctcgccga ccgtcgcgaa
     1321 cgtcagcttc agcggtcacg tcggcgggtc cacgacgacg acgggcgccg gcgggacgtt
     1381 cacgttcacc acgaccgaca cgatcacctt ccagatcgtc gtcagccggg acggcgtcga
     1441 cttcagcacg gcgacgctgg cgaacgcggt cgtcacgggt gtcgccggga cggggacgca
     1501 cacgatcacc tgggacggga aggaccaggg catgacgaac ttcccgtcga gcgcgacacc
     1561 ctattccttc cgagtctacg gccgcgccgg cgagatccat ttcccgatca tcgacgccga
     1621 gggaaacccc ggcgccgggc cgatcgtcac tcgtctcaac ggaaccgggg ccccgagcac
     1681 gaccgtcttc ttcgacgacc gcggctacgt cacctcgagc gggacgaccg tcggcgtcct
     1741 caacggcacg ctctgcaacg cgacgccgcc gactcctccc agcccgcagt tcgctctgga
     1801 cggcgtcgac tcctcgtcca ccgcgggcgg cggataccgc gactggctcg ggaacggcaa
     1861 ctcgaacagc gactgcgcgg cgaccgccgg gtggggcgac gcgaaggctc tcaacatctg
     1921 gacctactac tcggctccgt tcgagacgaa gacgctcgtc gtcgacccga tcatcatcga
     1981 cgtcgcgacg acggtctcgg cgcctgcctc ggccgtggcc gggagcaccg tccagggggc
     2041 gttcagcttc gggaacaacg ggaactccac ggcactcggc gtcacgtacg gcatgaccct
     2101 gacgcccggg ctcgggacgg tcaccttcgg caacctcccg ggaggcgtca cggcgacgta
     2161 caacaacgtc accggggccg tcaccttcac ggggcttccg acgtcgctga cgcccggcca
     2221 ggcgatctcg gggatgacgt tcagctacac ggcacccgcg agcggcccgg tcgtcgcaac
     2281 gacgtcggtc ggaacgacgt cgacggattc gtacctcccg aacaacacgg cgacggtctc
     2341 gacggggatc ggcgccgtcg acgtcctgac gacggtgagc gttccggcga cggcgacgac
     2401 ggggagcacg gtgagcggct ccttccagtt cgcgaacttc ggcgcgaatg ccgcgggctc
     2461 ggtgacgtac tccgccacga tcggcgcgcc gggcaactac cccgcgtccg tctccttcac
     2521 ggccctcccg ttcggcgtga cggcgaccta caaccccgtc aacggccagg tgactttcac
     2581 ggggatgccg acgaccctcg cgagcggcca gcagctgggc ttcggcttca gctatccggc
     2641 gccggggccc ggaaccgttc cggtcgccac gacgatcacg acggcctctt cggacgcgaa
     2701 ccccctgaac aacgcggcgg ccgacgcgac gacgatcgcc gcgcccgatc tcacgatcac
     2761 gaagagcgac ggcggggcga cgttcactgt cggcctgaac ggcacctaca cgctcactgt
     2821 cacgaacacg gggacggccg aaaccacggg cgacatcacg gtcacggaca ccttaacggc
     2881 ggggctcacg ttcgtctccg gcatggggcc ctccttctcc tgttcggcgg ccctccaggt
     2941 cgtcacgtgc acgcgactcg ccgcgaatcc catcgccatc ggcacgtccg ttccgatcac
     3001 gctcgtcgtg tcggtcgggc cggcggccct gcccggcgtg acgaactcgg ccacggtctc
     3061 gacgccgaac gagtcgaaca cgggcaacaa cacgggaagc gacacgactc cggtcgcggc
     3121 gacggcccac ctctcgatca cgaagaccga cggggtcgcg acggtcgtgc cgggtagcag
     3181 cgtcacctac accatcgtcg cttcgaacgc cggcccgtc
//
LOCUS       DAOTEZ010000479         3214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_23063_length_3214_cov_6.501425, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000479 DAOTEZ010000000
VERSION     DAOTEZ010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23063_length_3214_cov_6.501425"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2365
                     /locus_tag="P5164_18095"
     CDS             <1..2365
                     /locus_tag="P5164_18095"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRY45848.1"
                     /translation="VAVALFLLTLRASRPKSPDLVAAKEALRAGDFGRAGELYLKLGD
                     VDEAKRAFRKARLPARVAALELRQGNARAAAELWEEAGDAWSWEAARAWEAAGEPAKF
                     ESARKRALAEARQAARWDRLAEVAEAVGDRAGLADATRKIAEAAKPGGGRSALWKRAA
                     EAARAAGRTLEAAEAFRFAGEPLEAGALFLEADRPQDAARELERAGDLAGAAEAWSRA
                     GQTKTAAELRARDLEGQADWSGAADAWAEAANWEKAALMYERAIRPADAARAFGRAGR
                     HERAAALFQKAGLLAEAAAAFEAAAKPEAAAALYKDLREWERSISLYRASGRFAEAAQ
                     LLQEQGSFEEATSLFLRAGRNLDAARSALRNGSREKAWDLLTTVRRADAGVAEVFLEL
                     GEAHLANGEPKDAVHVLRELLGHKPVDAATIEAQEAFARALEASGDLAGAHAKLTQIA
                     DVQPGFRNAFSRAVALDRRLAEGGVPVAPAVETSAPVAAWPAPSSLAPVPPSAPGRDP
                     SGTFQAMATPGGIAAADRPEARYEIVAEVGRGGMGIVHKAFDHKLERHVALKILPGQL
                     WGDETAMRYFIREARAIAALKHPNIVGLYDFGEGFGSAYLAMEFLEGPNLQSLLKNDP
                     ERLKKSWRDYFVQSARGLAAAHAKGILHRDLKPANLVLDEHGILRILDFGLARPEADS
                     GSTSKLIGTPAFFPPEVLRGEAASPASDVYSLGATFYTLAAGRWPYVGEDVLVARLER
                     DPDDPRPFAPWLHEDEVLVLLRSISRHRPERYQDGGELLGALLSLEA"
     gene            complement(2349..2870)
                     /locus_tag="P5164_18100"
     CDS             complement(2349..2870)
                     /locus_tag="P5164_18100"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRY45849.1"
                     /translation="MPSLPKLPTLAAAPDPATVCPPCGKCCRYVAVGIDGPVTVSGVS
                     TALWLVYHRNVSVYQSHDGDWFVLFPADCENLLPSGLCGVYENRPTLCRDYDVDGCEG
                     TSAEAPEKVRFDDGAALGAWLRSRRPALFARCLAKGILPPAVRGDAPAQPSGAASRGN
                     PASGRRAPVRPRG"
     gene            2904..>3214
                     /locus_tag="P5164_18105"
     CDS             2904..>3214
                     /locus_tag="P5164_18105"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRY45850.1"
                     /translation="MKAALLTSHGGNEVLSVGSRPDPAPGPGEVLVRVSAAALNRLDL
                     FVRNGMPGVPVAFPHVPGADGCGTIEAVGDGVPGISVGERVVLQPGVSCGSCEFCRDG
                     ER"
BASE COUNT          432 a         1138 c         1249 g          395 t
ORIGIN      
        1 cgtcgccgtc gcgctctttc tcctcacgct ccgcgcgtcc cggccgaaga gcccggacct
       61 ggtcgccgcg aaggaggccc ttcgagccgg cgacttcggc cgtgcgggcg agctctacct
      121 gaagctcgga gacgtggacg aagcgaagag ggcgttccgc aaggcgaggc ttccggcgcg
      181 ggtcgccgcc ctcgagctcc ggcaggggaa cgctcgcgcc gcggccgagc tgtgggagga
      241 ggcgggcgac gcctggtcct gggaggccgc ccgcgcctgg gaggcggccg gggagcctgc
      301 gaagttcgaa tccgcccgga agcgcgccct cgccgaggcg cgccaggcgg cgcgctggga
      361 ccgtctcgcc gaggtggcgg aggcggtcgg ggatcgcgcc gggctcgcgg acgcgacgcg
      421 aaagatcgcg gaggccgcga agcccggtgg cggacggagc gcgctctgga aacgtgccgc
      481 ggaggcggcc cgagccgccg gtcggacgct tgaggccgcc gaggcgtttc gtttcgcggg
      541 ggagcctctc gaggcgggcg cgcttttcct cgaggccgac aggccgcagg acgcggcacg
      601 cgagctggag cgggccgggg acctcgccgg cgccgccgag gcctggtctc gagccgggca
      661 gacgaagacg gccgccgagc tgcgggcccg ggacctcgaa gggcaggccg actggagcgg
      721 tgcggccgac gcctgggccg aggccgcgaa ctgggagaag gccgcgctca tgtacgaacg
      781 ggcgatccgg cctgcggacg cggcccgggc gttcggccgt gcgggccggc acgagcgggc
      841 ggcggcgctg ttccagaagg cgggcctcct ggccgaggcg gccgcggcgt tcgaggcggc
      901 ggcgaagccg gaggcggccg cggcgctcta caaggacctc cgcgagtggg agcgctccat
      961 ctccctctac cgcgcctcgg ggcgcttcgc cgaggcggcc cagctcctgc aggagcaggg
     1021 gagcttcgag gaggcgacct cgctcttcct ccgcgccggc cggaacctcg acgcggcgcg
     1081 cagcgcgctc cggaacggct cgcgggagaa ggcctgggac ctcctgacga cggtccgccg
     1141 ggccgacgcg ggggtggccg aggtcttcct cgagctcggc gaggcgcacc tcgcgaacgg
     1201 cgagccgaag gacgcggtcc acgtcctccg ggagctcctc gggcacaagc cggtcgacgc
     1261 ggcgaccatc gaggcgcagg aggcgttcgc ccgggccctc gaggcgtcgg gcgacctggc
     1321 cggcgcgcac gcgaagctga cccagatcgc ggacgtgcag ccggggttcc ggaacgcctt
     1381 ctcgcgggcg gtcgcgctcg atcggcgcct cgccgaaggg ggcgtcccgg tcgccccagc
     1441 cgtcgagacc tccgcaccgg tcgcggcgtg gccagcgccg tccagcctcg cccccgtgcc
     1501 gccgtcggcg ccgggaagag acccgtcggg taccttccag gcgatggcga cccccggcgg
     1561 aatcgcggcg gcggaccggc ccgaggcgcg ctacgagatc gtcgccgagg tgggccgcgg
     1621 cggcatgggg atcgtccaca aggccttcga ccacaagctc gagcgccacg tcgcgctcaa
     1681 gatccttccc gggcagctct ggggcgacga gacggcgatg cgctacttca tccgcgaggc
     1741 gcgggcgatc gcagcgctca agcacccgaa catcgtcggc ctctacgact tcggggaggg
     1801 gttcggaagc gcctacctcg ccatggagtt cctggaagga ccgaacctcc agtcgctcct
     1861 caagaacgac ccggagcggc tgaagaagtc ctggcgggac tacttcgtcc agtcggcgcg
     1921 gggcctcgcg gccgcgcacg ccaaggggat cctccaccgc gacctgaagc cggcgaacct
     1981 cgtgctggac gagcacggga tcctccggat cctggacttc ggactggctc ggcccgaggc
     2041 cgattccggc tcgacctcca agctgatcgg gacgccggcc ttcttccctc cggaggtcct
     2101 gcggggggag gcggcgtccc ccgcctcgga cgtctactcg ctcggcgcca cgttctacac
     2161 gctcgctgca ggccgctggc cgtatgtcgg cgaggacgtc ctggtcgcgc gactcgagcg
     2221 cgacccggac gacccgcggc cgttcgcgcc ctggctccac gaggacgagg tcctcgtcct
     2281 ccttcgctcc atctcccgac accgccccga gcgctaccag gacggcggcg agctgctcgg
     2341 cgcgctcctc agcctcgagg cctgacgggg gcgcggcggc ccgacgcggg attcccacgg
     2401 ctcgccgcgc ccgacggctg ggccggagcg tcgccccgca ccgcaggcgg gaggatcccc
     2461 ttggcgaggc accgcgcgaa gagggcgggg cgccgcgaac gcagccaggc gccgagggcg
     2521 gctccgtcgt cgaagcggac cttttccgga gcctccgcgc tcgtcccctc gcagccgtcg
     2581 acgtcgtagt cgcggcagag ggtcgggcgg ttctcgtaga cgccgcagag gccgctcggc
     2641 aggaggttct cgcagtcggc ggggaagagg acgaaccagt cgccgtcgtg cgattggtag
     2701 accgagacgt tcctgtggta aacgagccag agcgccgtcg agacgccgct gacggtcacg
     2761 gggccgtcga tgccgaccgc gacgtaacgg cagcacttgc cgcagggcgg gcagacggtg
     2821 gcgggatcgg gggcggcggc gagggtcggc agcttcggca ggctcggcat cgaaggcggg
     2881 agtttccacg ggaaggggga gaaatgaaag cggctcttct gacgtcccac ggcggcaacg
     2941 aggtcctctc cgtcgggtcg cgtccggacc cggcccccgg ccccggcgag gtcctcgtga
     3001 gggtctcggc cgccgcgctg aaccggctcg acctcttcgt gaggaacggg atgcccggcg
     3061 tcccggtcgc gttcccgcac gtccccgggg ccgacggatg cgggacgatc gaagcggtcg
     3121 gcgacggcgt cccggggatc tcggtcgggg agcgagtcgt cctgcagccg ggagtctcgt
     3181 gcgggagctg cgagttctgc cgggacggcg agcg
//
LOCUS       DAOTEZ010000480         3207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_23159_length_3207_cov_5.852475, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000480 DAOTEZ010000000
VERSION     DAOTEZ010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23159_length_3207_cov_5.852475"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1802)
                     /locus_tag="P5164_18110"
     CDS             complement(<1..1802)
                     /locus_tag="P5164_18110"
                     /inference="COORDINATES: protein motif:HMM:NF018662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 domain-containing protein"
                     /protein_id="HRY45851.1"
                     /translation="MTRSAALAALAAAFLAPMARTASAIDGKQGDLSWSWDTTISYGL
                     FTRLEKRDPAIVGLRSGGTAFSANGDDGNLNYDTGLASSAFKATTELELTYKRIGAFV
                     RAYGFYDIENEERDRDRTPLSPEALDRVGSRAEIRDAFLWAKFDLGPVPGEIRAGWQV
                     INWGESTFIQGGINAINPVDVSALRVPGSELRDALLPVGAVLLSVKPSKNTSLEGFWQ
                     YAWTQTKIDPVGSYFSTTDLAGAGATKVMLGFGSAPDSIPVGFNIPNNPVGVAVPRAG
                     DREASDSGQYGAAFRLFVPALGGTEFGLFYMNYHSRLPLIMARTGTQAGLLARGNYAA
                     SASYFLSYPEDIKLYGLSFNASLGSTGVALQGEVSHRKDVPLQVDDVEILYAALTPLR
                     LLPAAPQLAPLRQLGGLLAATNQAGAYGFDEEIRGYRLFDTTQVQLTATRVFGRVLGA
                     DQLVLVAEGAWNKVHDLPEQSVLRLEAPGTYTSGNPVHQQAGVQPGTEPSTAFPTSSS
                     WGYVVAGRLDYSNAIGAINLAPRFSFAHDVNGVSPGPGGSFIEDRMALTLGLGFQYRI
                     NVEWDLSYTRYFGAGRYNLINDRDFLAGNVKYSF"
     gene            complement(2005..>3207)
                     /locus_tag="P5164_18115"
     CDS             complement(2005..>3207)
                     /locus_tag="P5164_18115"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HRY45852.1"
                     /translation="YRFEVLDPATGRLLYARGYSSIYAEWETIPEAKAAHRTFRESLR
                     FPWPLRPVQVVLKKRDRRNLFREVWGTLVDPASPDVNPAPPPRSGTVWTVFESGPAAE
                     KVDLLVLGEGYAEKDLPKFRADVKRMVDTLFRYEPFRSRRADFNVRALDLPSGEGGAH
                     RPQSKIFRRTPLQVQYNVFGSERYVLTYDDRALRDAAAQAPYDALEILVNDAQYGGGG
                     IYNHQATASADTAFAEYVFVHEFGHHFAALADEYYTSDVAYETGAADLPEPWEMNVTA
                     LKDPANPKWKELVEAGTPLPTPWSKEAFEKASREFQARRKALLAAGKPPAEIDALFRE
                     QQKAEAAFFAKERWAGTVGAFEGAAYEAKGLYRSSADCIMFTRTTGGFCPVCRKAIEK
                     VIDSHARP"
BASE COUNT          457 a         1034 c         1208 g          508 t
ORIGIN      
        1 tagaaggagt acttcacgtt gcccgcgagg aagtcgcggt cgttgatcag gttgtaccgg
       61 cccgcgccga agtagcgggt gtagctcagg tcccactcga cgttgatccg gtactggaag
      121 ccgagaccga gcgtcagcgc catccggtcc tcgatgaagg agccgcccgg cccgggcgag
      181 acgccgttca cgtcgtgggc gaaggagaag cgcggggcga ggttgatcgc gccgatggcg
      241 ttgctgtagt cgagccggcc ggccacgacg tagccccagg acgagctcgt cgggaaggcg
      301 gtcgagggct cggtgcccgg ctggacgccc gcctgctggt ggaccgggtt gccgctcgtg
      361 taggtacccg gagcctcgag ccggaggacg gactgctcgg ggaggtcgtg gaccttgttc
      421 caggcgccct cggcgacgag gacgagctgg tcggcgccga ggacgcggcc gaagacccgc
      481 gtcgcggtga gctgaacctg ggtcgtgtcg aagaggcggt agccgcggat ctcctcgtcg
      541 aagccgtagg cgccggcctg gttcgtggcg gccaggaggc ctccgagctg ccggagcggc
      601 gccagctgcg gcgccgccgg gagcagccgg agcggcgtga gcgccgcgta caggatctcg
      661 acgtcgtcga cctggagggg gacgtccttg cgatgggaga cctctccctg gagcgcgacg
      721 ccggtgctgc cgaggctcgc gttgaagctg aggccgtaga gcttgatgtc ctccggatag
      781 gagaggaagt agctcgcgct cgccgcgtag ttgccccggg cgaggaggcc cgcctgggtc
      841 ccggtacgcg ccatgatgag cggcagccgg ctgtggtagt tcatgtagaa gaggccgaac
      901 tcggtcccgc cgagggcggg gacgaagagc cggaacgcgg cgccgtactg gccggagtcg
      961 ctcgcctcgc ggtcgccggc gcgcgggacc gcgacgccga ccgggttgtt cgggatgttg
     1021 aagccgacgg ggatcgagtc cggggcggag ccgaacccga gcatgacctt cgtcgctccg
     1081 gcgcccgcga ggtcggtcgt ggagaagtag ctcccgaccg ggtcgatctt cgtctgggtc
     1141 caggcgtact gccagaatcc ttcgagggag gtgttcttcg agggcttgac gctcaggagg
     1201 acggccccga cggggaggag ggcgtcgcgc agctccgagc cggggacgcg cagggcgctg
     1261 acgtccacgg ggttgatcgc gttgatgccc ccctggatga acgtgctctc gccccagttg
     1321 atgacctgcc agccggcgcg gatctctccc gggacgggcc cgaggtcgaa cttcgcccag
     1381 aggaaggcgt cccggatctc cgcccggctg ccgacccggt cgagggcttc ggggctcagc
     1441 ggggtgcggt cgcggtcgcg ctcctcgttc tcgatgtcgt agaagccgta ggccctgacg
     1501 aacgcgccga tccgcttgta cgtcagctcg agctccgtcg tcgccttgaa ggcgctgctc
     1561 gcgaggcccg tgtcgtagtt gaggttcccg tcgtcgccgt tcgcggagaa ggccgttccc
     1621 ccggacctga ggccgacgat cgcggggtct ctcttctcga gccgcgtgaa gaggccgtac
     1681 gagatcgtcg tgtcccagct ccaggagaga tccccctgct ttccgtcgat cgcggacgcc
     1741 gtccgggcca tgggggccag gaaggccgcg gcgagagccg caagtgcggc actccgtgtc
     1801 atctgtcgaa atgcgctcat caggtccccc ttcgccaatc tgcgggcttg gcgcggaggg
     1861 taacccagag gacgcggatt tcgcacagaa accgtgctcg gcgtcacaaa cgcgctaacg
     1921 cgcggccagg caagtgtact gcgtttacgc aacgaggaga cgcggtccgg gccgccgggt
     1981 gcggcccgga tccggctgcc cggctcaggg ccgcgcgtgg ctgtcgatga ccttctcgat
     2041 cgccttccgg cagaccgggc agaagcctcc cgtcgtccgc gtgaacatga tgcagtcggc
     2101 cgaggagcgg tagagcccct tcgcctcgta ggcggccccc tcgaaggcgc cgaccgttcc
     2161 ggcccacctc tccttcgcga agaaagccgc ctcggccttc tgctgctcgc ggaagagggc
     2221 gtcgatctcc gccgggggct tgccggcggc gaggagcgct ttcctccggg cctggaactc
     2281 gcgcgaggcc ttctcgaacg cttccttcga ccagggggtc gggagcggcg tccctgcctc
     2341 gacgagctcc ttccacttcg ggttcgccgg gtccttcagc gccgtcacgt tcatctccca
     2401 cggctcgggg aggtcggcgg cgcccgtctc gtaggcgacg tcggaggtgt agtactcgtc
     2461 ggcgagggcg gcgaaatggt ggccgaactc gtggacgaag acgtactcgg cgaacgcggt
     2521 gtcggcggag gcggtcgcct ggtggttgta gatgccgccg ccgccgtact gggcgtcgtt
     2581 cacgaggatc tccagggcgt cgtacggcgc ctgggccgcc gcgtcgcgca gggcgcggtc
     2641 gtcgtaggtg aggacgtagc gctcggagcc gaacacgttg tactgcacct ggagcggcgt
     2701 ccgccggaag atcttcgact gcgggcggtg cgcgccgccc tcgccggacg ggaggtcgag
     2761 ggcgcggacg ttgaagtcgg ccctgcgcga tcggaacggc tcgtagcgga agagggtgtc
     2821 gaccatccgc ttcacgtcgg cgcggaactt cgggaggtcc ttctcggcgt atccctcgcc
     2881 gaggacgagg aggtcgacct tctccgcggc gggaccgctc tcgaagacgg tccagaccgt
     2941 cccgctcctc ggcggcggcg ccgggttcac gtcgggcgag gcggggtcga cgagcgttcc
     3001 ccagacctcc cggaagaggt tccgccggtc gcgcttcttc aggacgacct ggacgggacg
     3061 gagcggccag gggaagcgga gcgactcgcg gaacgtccgg tgcgccgcct tcgcctcggg
     3121 aatcgtctcc cactcggcgt agatcgagga gtagccgcgc gcgtagagga ggcggcccgt
     3181 cgccgggtcg aggacctcga agcggta
//
LOCUS       DAOTEZ010000481         3179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_23490_length_3179_cov_4.205826, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000481 DAOTEZ010000000
VERSION     DAOTEZ010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23490_length_3179_cov_4.205826"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1679
                     /locus_tag="P5164_18120"
     CDS             <1..1679
                     /locus_tag="P5164_18120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY45853.1"
                     /translation="DDVRAALARITAEVDERLEWSSGSGRYRLESVAAGATHEGTLPA
                     GQSPATLLSSELVFVARGDAADLPPAHLETIAATAEAEGLGVVAVAAPQGDPLSSQSV
                     LMRRELWSTVRPPTGPILGKVVPALGPPSPSATRSLAESFRRLGVASARGVDRYAVDA
                     PAGGGAIPHTIAPIGATLGAAARPDGRTTVLLLLPYVAVGGVEKLTLDMMRSLGDGYR
                     WVVVSLVPHDPLLGNRLEEFRAITPWVYLLGEALPPQLYFSAISRLLESHRVDVLFSV
                     NGTTWFFEALGTLRQSFPRLRIASQLYDHEVGWIEHYHGDVGKQIDVTVAINKRIART
                     LVEKHRIPADRVVVNFGGIDLSVFEPGRIPPERREALRRELGVPGEAILVTLAARMHA
                     QKRPLDFVALARRFRDRPEYFFLLAGGGPLEASVDARIAEAPENVKRVPFVEGMAELW
                     AASDVGCLVSEYEGLPLVVLEALAMGKPFLSTDVGGVREILEEGPCGVVVRKPGDLDG
                     LERGLLELGPAEARKRMGREGRRIVERDFTIEAAGRIYAEVLRPSTERAG"
     gene            1679..2617
                     /locus_tag="P5164_18125"
     CDS             1679..2617
                     /locus_tag="P5164_18125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY45854.1"
                     /translation="MARVSVVIPSYQHEHFLGAALDSLEAQTRPPDEIVVVDDGSKDG
                     SVALVKARLRPGLRLLEQENRGAHAALNRGIEEATGEILFLLNSDDRFAPERVEKLAS
                     LLEEGPGAGLVSSWLRLIDAKGKALGVKEAWRNMHPWPARRPERTFVATPNARWNLLQ
                     GNYLATTSNFAFRRELWEKAGPFRPLRFAHDWDFALTAALDAAPTVVEEPLVEYRIHE
                     KNTIRTDPKEMELEALWVVARHLGRFAARETTGTPEGSREFLDRLLESFHAFGKERLF
                     TLLVALAGDDSPGSAFSSLLERESPLRRWALEELSR"
     gene            complement(2622..>3179)
                     /locus_tag="P5164_18130"
     CDS             complement(2622..>3179)
                     /locus_tag="P5164_18130"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY45855.1"
                     /translation="CRRRFAGEKHLEYHVNDGRSLEMVEDGSVDFAFSFDSLVHVELD
                     VLDGYLRQLAKKLKPEGVAFIHHSNIGEHVEYLEKLKRWGVKDGHPLVKWGLAHPADH
                     WRARSVTAAKFRSAAEGAGLRCLTQEIVNWGTPRLIDCLSTLALPGSGWRKETVVVRN
                     PRFMDEAEEIRQKAPLYARTGLRAG"
BASE COUNT          431 a         1130 c         1153 g          465 t
ORIGIN      
        1 ccgacgacgt gcgcgccgcg ctcgcccgga ttaccgccga ggtcgacgag cggctcgagt
       61 ggagctccgg cagcggccga taccggctcg agagcgtcgc ggcgggcgcg acccatgagg
      121 ggacgcttcc ggccgggcag tcccccgcta cgctcctctc gtcggagctc gtcttcgtcg
      181 ctcgcggcga cgccgcggac ctgcccccgg cccacctcga gacgatcgcc gcgacggcgg
      241 aggccgaggg actcggcgtc gtcgccgtcg ccgcgccgca gggcgatccg ctctcgtcgc
      301 agtcggtcct catgcgacga gagctctgga gcacggtgcg tccgcccacc ggcccgatcc
      361 tcggaaaggt cgttcccgcc ctcggtccgc cgagcccctc ggcgacccgg tcgctcgcgg
      421 agtcgttccg ccggctcggc gtggcctcgg cccgcggggt cgatcggtac gccgtcgacg
      481 ccccggcggg cggcggagcg attccgcaca cgatcgcccc gattggcgca accctcggcg
      541 cggcggctcg tccggacggc cgcacgaccg tcctcctcct ccttccgtac gtcgccgtcg
      601 gaggcgtcga gaagctcacg ctcgacatga tgcggtcgct cggcgacggc taccggtggg
      661 tcgtcgtgtc gctcgtaccg cacgacccgc tcctcgggaa ccggctcgag gagttccggg
      721 cgatcacgcc ctgggtctac ctgctcgggg aagcgctccc cccgcagctc tacttctcgg
      781 cgatctcgcg gctcctcgag agccaccgcg tcgacgtcct cttcagcgtg aacgggacga
      841 cctggttctt cgaggcgctc gggacgctcc ggcagagctt cccccgcctg agaatcgcga
      901 gccagctcta cgaccacgag gtgggctgga tcgagcacta ccacggcgac gtcggaaagc
      961 agatcgacgt cacggtggcg atcaacaaga ggatcgcccg gacgctcgtc gagaagcacc
     1021 gcatcccggc ggatcgtgtc gtcgtgaact tcggcgggat cgacctctcg gtcttcgaac
     1081 cgggccgcat cccgcccgag cggcgcgagg cgctccgccg cgagctcggc gtccccgggg
     1141 aggcgatcct cgtcacgctc gcggcccgga tgcacgccca gaagcgcccg ctcgacttcg
     1201 tcgcgctcgc ccggcgcttc cgcgaccggc cggagtactt cttcctcctc gccggcggcg
     1261 gccccctcga ggcgagcgtc gacgcgcgga tcgccgaggc cccggagaac gtaaagaggg
     1321 tcccgttcgt ggaggggatg gcggagctgt gggccgcgag cgacgtcggg tgcctcgtct
     1381 cggagtacga agggctgccg ctcgtcgtcc tcgaggcgct cgcgatgggg aagccgttcc
     1441 tcagcaccga cgtcggcggg gtgagggaga tcctggaaga ggggccgtgc ggggtcgtcg
     1501 tccggaagcc cggcgacctc gacgggctcg agaggggcct cctcgagctg gggccagccg
     1561 aggcccggaa gcggatgggc cgggaggggc ggcggatcgt ggagcgcgat ttcacgatcg
     1621 aggcggcggg gcggatctac gcggaggtcc tgcgcccctc gacggagcgg gcgggctgat
     1681 ggcgcgggtc tccgtcgtca tcccgtcgta ccagcacgag cacttcctcg gcgccgcgct
     1741 cgacagcctg gaggcgcaaa cgaggccgcc cgacgagatc gtcgtcgtgg acgacggctc
     1801 gaaggacggc tccgtcgccc tcgtgaaggc acggcttcgc cccgggctgc gtctcctcga
     1861 gcaggagaac cgcggcgccc acgcggcgct gaaccgggga atcgaggagg cgacgggcga
     1921 gatcctcttc ctcctgaact ccgacgaccg gttcgcgccg gagcgcgtgg agaagctggc
     1981 gagcctcctc gaagaggggc cgggtgcggg tctcgtttcc tcctggctcc gcctgatcga
     2041 cgcgaagggg aaggcgctcg gcgtgaagga ggcctggcgg aacatgcacc cctggcccgc
     2101 ccggcggccg gagaggacgt tcgtcgccac gccgaacgcg cgctggaacc tcctccaggg
     2161 gaactacctc gcgacgacct cgaacttcgc gttccgccgg gagctctggg agaaggccgg
     2221 cccgttccgt ccgctgcggt tcgcccacga ctgggacttc gccctgaccg ccgccctcga
     2281 cgccgcgccg acggtggtcg aggagccgct cgtcgagtac cggatccacg agaagaacac
     2341 gatccggacc gacccgaagg agatggagct ggaggccctc tgggtcgtcg cgcggcacct
     2401 cgggcgcttc gcggcgcgcg agacgaccgg gacgcccgag gggagccgcg agttcctcga
     2461 ccggctcctg gaatcgttcc acgccttcgg gaaggagcgg ctcttcacgc tcctcgtcgc
     2521 cctcgcgggc gacgattcgc ccggctcggc gttctcctcg ctcctcgagc gcgagagccc
     2581 gctccggcgc tgggcgctgg aggagctctc gcgctgacgc gtcagccggc ccgcaggccg
     2641 gttctggcgt aaagcggcgc cttctggcgg atctcctcgg cctcgtccat gaagcgggga
     2701 ttgcggacga cgacggtttc cttccgccag ccggagcccg ggagcgcgag ggtgctgagg
     2761 cagtcgatga ggcggggcgt gccccagttc acgatctcct gggtgaggca gcggaggccg
     2821 gccccttcgg cggccgagcg gaacttcgcc gccgtgacgc tccgggccct ccagtggtcg
     2881 gccgggtggg cgaggcccca cttcacgagc ggatggccgt ccttcacgcc ccagcgcttc
     2941 agcttctcga ggtactcgac gtgctcgccg atgttcgagt ggtggatgaa cgcgacgccc
     3001 tcgggcttca gcttcttcgc gagctggcgg aggtagccgt cgaggacgtc gagctcgacg
     3061 tggacgaggg agtcgaagct gaaggcgaag tcgacggagc cgtcctcgac catctcgagc
     3121 gagcggccgt cgttgacgtg gtactcgagg tgcttctcgc ccgcgaagcg ccggcggca
//
LOCUS       DAOTEZ010000482         3165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_23651_length_3165_cov_6.844051, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000482 DAOTEZ010000000
VERSION     DAOTEZ010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23651_length_3165_cov_6.844051"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /locus_tag="P5164_18135"
     CDS             complement(<1..1361)
                     /locus_tag="P5164_18135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRY45856.1"
                     /translation="MHPGPSGSSERAPGRARPAHALGAAFPVLVLALLFAAPTSRASV
                     DPARSLAQSTLDSWETGQGLPQNTVQAIAQTPEGYLWIGTQEGLVRFDGTQFTLFDQR
                     TNAELSVSSVFALLADLDGALWVGTNGGGLLRMTGSRFERFGPETGLTGLKIQALARD
                     GRGALWVGTVDRGLFRRVGERFERVRVGAGPHEMPVRTLCVDRSGAVWAGTMGGGVSR
                     LDPDGPVSFTTREGLPNDTVVALAAHPAGGVWAGTAGGGLARIADGKVLASGAPALPT
                     GQVSALLVDDAGNLWAGTWGRGVCLVSGEKVACQSARTGDLPSDHVWSLFQDRERSVW
                     IGTWTDGLLRLRHGVFASFGRREGLAGENVRAVAEDASGTIWATVASGGLQRISGTSI
                     RSFGTADGLPSDQTSALLPARDGRLWVGTYTDGLAVGRDGVFRRFALPPPEWASRDVR
                     ALV"
     gene            complement(1411..>3165)
                     /locus_tag="P5164_18140"
     CDS             complement(1411..>3165)
                     /locus_tag="P5164_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRY45857.1"
                     /translation="GGRHMSNHFARHDLYVWNVSSCVSNHAQHAAGLGRAIRSYGLQS
                     LVYCSMGESSTSEGYFYEAVNGSSLGKLPVIFVIQDNGYGISVPKRQQTANHYASDNF
                     ASFPNLKVVHCDGTDVLDSVRALREAAAFAQSGAGCVLVHADCVRIGSHSNSDRHELY
                     RDDEELAAARERDPLPRFRRFLLESGGFSEEELRAIEDENARTYESAADRARTAPDPD
                     PRSIHDFVLPEPWVPDGDGLADVPRTPPASADGRANRESTLIHAINETMKDLFRENSD
                     TFIWGQDVASKEKGGIFNVTKGMEKEFGSHRVFNAPIAEDYILGMADGFSRVCDGVRV
                     IVEGAEFADYFWPAAEQMVEMSHEYWRTNGQFVPNVTVRLASGGYIGGGLYHSQNVEG
                     WLTTLPGIRIVVPAFADDAAGLLRTAVRSRGITLYLEPKFLYNSPLAKAFVPDGFAVP
                     FGKARLRRPGTDLTIVAYGTPVHWALEAAGTLAKEGKEVEVIDLRSLAPLDFEAIADS
                     VRKTSRLVIAHEDKVQGGFGGEIAARVTSELFEYLDAPVERVGSTFTPVGFNRILERA
                     ILPDTARVLAAARKVLAY"
BASE COUNT          459 a         1089 c         1183 g          434 t
ORIGIN      
        1 tcgacgaggg cgcgcacgtc gcgcgacgcc cactccggcg gcgggagggc gaaccgccgg
       61 aagacgccgt cgcggccgac tgcgagcccg tcggtgtagg tgccgaccca gagcctcccg
      121 tcgcgggccg gaaggagggc ggaggtctgg tcggagggga ggccgtccgc cgtcccgaag
      181 gagcggatcg aggtccccga gatccgctgg agccctccgc tcgccaccgt cgcccagatc
      241 gtccccgagg cgtcctccgc gacggcgcgg acgttctctc cggcgagccc ctcccgacgc
      301 ccgaagctcg cgaagacccc gtgacgcagg cggaggagac cgtccgtcca ggtgccgatc
      361 cagacgctcc tctcgcggtc ctggaagagg ctccagacgt ggtcggacgg gaggtcgccg
      421 gttcgggccg actggcaggc gactttctct cccgagacga ggcagacgcc gcgaccccag
      481 gtaccggccc agaggttgcc ggcgtcgtcg accagaagcg cggagacctg tccggtcggg
      541 agggccggcg cgccgctcgc gaggaccttc ccgtcggcga tccgcgcgag gccgccgccg
      601 gccgtgccgg cccagactcc tcccgccgga tgggccgcca gggcgacgac ggtgtcgttc
      661 ggaaggccct ctctcgtcgt gaacgagacc ggtccgtccg gatcgagtcg ggagacgccg
      721 ccccccatcg tcccggccca gacggctccg gagcggtcga cgcagagcgt gcggaccggc
      781 atctcgtgcg gaccggcgcc gacccggacc ctctcgaagc gttcgccgac gcgccggaag
      841 agccctcgat cgacggtccc gacccagagc gcgccccgtc cgtcgcgggc gagggcctgg
      901 atcttcagtc ccgtcagacc ggtctccggg ccgaacctct cgaaccggct gccggtcata
      961 cggaggaggc cgcctccgtt cgtcccgacc cagagcgctc cgtcgaggtc ggcgaggagc
     1021 gcgaagacgg agctgaccga gagctcggcg ttcgtccgct ggtcgaacag ggtgaactgc
     1081 gtcccgtcga accggacgag gccttcctgg gtcccgatcc agagatagcc ctccggggtc
     1141 tgcgcgatcg cctggaccgt gttctgcggg aggccctggc ccgtttccca cgagtcgagc
     1201 gtcgactggg cgagggaacg cgccgggtcg acgctcgccc gggacgtggg cgcggcgaag
     1261 agaagcgcca gcacgaggac cgggaaggcg gcgccaagcg cgtgcgccgg ccgcgcccgt
     1321 cccggcgcgc gctcagagga gccgctcggc cccggatgca tccgccgatg atagagcggc
     1381 ccggccccga aggggcgggc cgccccgcgg tcagtaggcg aggaccttcc gggccgccgc
     1441 caggacgcgc gccgtgtcgg gaaggatcgc cctctcgagg atcctgttga agccgaccgg
     1501 cgtgaacgtc gagccgaccc gctcgacggg agcgtcgagg tactcgaaga gctcggacgt
     1561 gacccgcgcg gcgatctcgc cgccgaagcc cccctggacc ttgtcctcgt gggcgattac
     1621 gaggcgcgag gtcttccgga cggagtcggc gatcgcctcg aagtcgagcg gggcgaggga
     1681 gcgcaggtcg atcacctcga cctccttccc ctccttcgcg agcgttccgg ccgcctcgag
     1741 cgcccagtgg accggggtcc cgtacgccac gatcgtcagg tccgtgccgg gccgccggag
     1801 gcgcgccttc ccgaacggca cggcgaagcc gtcggggacg aaggccttcg cgagcgggga
     1861 gttgtagagg aacttcggct cgaggtagag ggtgatgccg cgggagcgga cggccgtccg
     1921 gaggaggccg gcggcgtcgt cggcgaaggc cgggacgacg atccggatcc cgggaagggt
     1981 cgtgagccag ccctcgacgt tctgcgagtg gtagaggccg cccccgatgt agccgcccga
     2041 ggcgagacgg acggtgacgt tcgggacgaa ctgcccgttc gtccgccagt actcgtggga
     2101 catctcgacc atctgctcgg cggcgggcca gaagtagtcg gcgaactccg ccccctcgac
     2161 gatgaccctc accccgtcgc agacgcgcga gaacccgtcg gccatcccga ggatgtagtc
     2221 ctcggcgatc ggggcgttga agacgcggtg gctcccgaac tccttctcca ttcccttcgt
     2281 gacgttgaag atcccgccct tctccttcga ggcgacgtcc tgtccccaga tgaaggtgtc
     2341 ggagttctcc cggaagaggt ccttcatcgt ctcgttgatc gcgtggatca gggtcgactc
     2401 gcggttcgcg cggccgtccg ccgaggcggg cggggtccgg gggacgtccg cgaggccgtc
     2461 cccgtccggc acccacggct cgggaaggac gaagtcgtgg atcgacctcg ggtcgggatc
     2521 gggcgcggtc cgggcccggt ccgccgcgga ctcgtaggtc cgggcgttct cgtcctcgat
     2581 cgcgcggagc tcctcctcgg agaagccgcc cgactcgagg aggaagcggc ggaagcgcgg
     2641 gagggggtcc cgctcgcggg cggcggcgag ctcctcgtcg tcgcggtaga gctcgtgccg
     2701 gtcggagttc gagtgcgagc cgatccggac gcagtcggcg tgcaccagga cgcagccggc
     2761 gccgctctgc gcgaaggctg cggcctcccg gagcgcgcgg acggagtcga ggacgtcggt
     2821 gccgtcgcag tggacgacct tcaggttcgg gaaggaggcg aagttgtccg aggcgtagtg
     2881 gttcgcggtc tgctggcgct tcgggaccga gatgccgtag ccgttgtcct ggatgacgaa
     2941 gatgaccggg agcttcccga gcgaggaccc gttcaccgcc tcgtagaagt aaccttccga
     3001 ggtcgaggac tcgcccatcg agcagtagac caggctctgg aggccgtagg agcggatcgc
     3061 gcggccgagg cccgcggcgt gctgcgcgtg gttcgagacg caggaggaga cgttccagac
     3121 gtagaggtcg tgccgggcga agtggttcga catgtgccgg ccgcc
//
LOCUS       DAOTEZ010000483         3165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_23656_length_3165_cov_4.165916, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000483 DAOTEZ010000000
VERSION     DAOTEZ010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23656_length_3165_cov_4.165916"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="P5164_18145"
     CDS             complement(<1..318)
                     /locus_tag="P5164_18145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF520 family protein"
                     /protein_id="HRY45858.1"
                     /translation="MADPSFDIVSEVAMPEVVNAVAQAQKEVAQRFDLKGTAAGIELK
                     EKERQIVLTANDDFGLKAVNDVLQGKLIKRGVHLKSLVYGTVEPAAKGTARQVVTIQQ
                     GIET"
     gene            complement(335..991)
                     /locus_tag="P5164_18150"
     CDS             complement(335..991)
                     /locus_tag="P5164_18150"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HRY45859.1"
                     /translation="MRRSFALPFLAAALASALVAAAPLTGDQTPDAPALLRAIVNTYK
                     ARPAFSMTFVQGYAPAGFPDTTPETGTLTIQAPASLRFDYDGAEGKVYTFDGRAARQY
                     VAMDKQIVLKTLTGAEKARLPLLFLQPASEVLDRFTAVATAAANGLVDLTLTPRAEGD
                     LKSVAVLATPSGEVRRLVVLDGEGNRTTFTFTNLAARAKRPLSDFALVPPKGTRVVGE
                     "
     gene            complement(1024..2568)
                     /gene="purH"
                     /locus_tag="P5164_18155"
     CDS             complement(1024..2568)
                     /gene="purH"
                     /locus_tag="P5164_18155"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRY45860.1"
                     /translation="MSTKRALLSVSDRSGLLDLANALLRHGFELVSTGGTGKVLSDAG
                     LPVTQVSDVTGFPEVFDGRVKTLHPALFGGILYDRSVGKHVEQAEANGVGPIDVVVVN
                     LYPFEETVAAVETTFAQGIEKIDVGGPSLLRAAAKNHAHVAVLCDPADYAAFAAELDA
                     NAGASTLGTRKRLAAKVFRRTAAYDAAISTWLSARTEEAPFAERLTLSFELGGTLRYG
                     ENPHQAAAFYRDPAAPPSALAGFVLHQGKELSYTNLLDADSALHTSRLLPRGAMTIVK
                     HRIPCGIAREESAAASFEAAWASDPVSGFGGVLAYTGVLDRAVAEAMTSKFLEVVVCE
                     GVTDEARAAFAKKPNLRVLTVAPDPAPRARRELRGIDGGVLVQEADVLPADAATWKVV
                     TKRAPTDAEMRALLFLERAVSGVISNAIVVGGETATYGIGGGRTSRVDAAGDAVSKAA
                     ARAKGACAASDAFFPFPDGLLLLADAGVTACVEPGGSVKDAEVIAAADERGMALLFSG
                     RRHFRH"
     gene            complement(2565..>3165)
                     /locus_tag="P5164_18160"
     CDS             complement(2565..>3165)
                     /locus_tag="P5164_18160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45861.1"
                     /translation="ALLVPLALLAAAPSPAAEVARWPLPAAAVMSADAAVNAPPDLKR
                     QLVKHRTRLMEGVKAGLAASPARDAAARRAALASGARRIAQAIRDHWRFEDVAFEVGG
                     LVAEVALLCGPPGPEPFDGDLVYFASERSVFRGFTARPFDAPETLVAAPLRTDNPREA
                     YDSGVTLATRLLAWIWNAAGGDASLALARPESKGPYPVR"
BASE COUNT          430 a         1056 c         1227 g          452 t
ORIGIN      
        1 ggtttcgatc ccctgctgga tcgtgacgac ctgccgggcc gtccccttcg ccgccggctc
       61 gaccgttccg tagacgaggg atttcaggtg gacgcctctc ttgatgagct ttccctggag
      121 gacgtcgttc accgccttga ggccgaagtc gtcgttggcc gtcaggacga tctggcgctc
      181 cttttccttc agctcgattc ccgcggcggt tcccttcagg tcgaagcgct gggcgacttc
      241 cttctgggcc tgagcgaccg cgttgacgac ctcgggcatg gcgacctcgg acacgatgtc
      301 gaagctggga tcggccatct ctttcctccg tccctcattc tccgacgacg cgggtcccct
      361 tcgggggaac cagggcgaaa tcggagaggg ggcgctttgc gcgtgcggcc aggttcgtga
      421 aggtgaaggt cgtccggttc ccctcgccgt cgaggacgac gaggcgccgg acctcgccgg
      481 agggggtcgc caggaccgcg accgacttca gatctccctc cgcgcggggg gtcagcgtca
      541 ggtcgacgag gccgttcgcg gccgctgtcg ccacggccgt gaaccggtcg aggacttcgg
      601 aagcgggctg gaggaagagg agggggaggc gagccttctc ggctcccgtg agggtcttca
      661 ggacgatctg tttgtccatc gcgacgtact gccgggccgc ccggccgtcg aacgtgtaga
      721 cctttccttc cgccccgtcg tagtcgaacc ggagcgaggc cggagcctgg atcgtcaacg
      781 tgccggtttc gggagtcgtg tcggggaagc cggcgggtgc gtagccctgg acgaacgtca
      841 tcgagaacgc gggcctcgcc ttgtaggtgt tcacgattgc ccggaggagc gccggggcgt
      901 cgggtgtctg gtccccggtc agcggcgcag cggcgacgag cgcggaggcg agcgccgcgg
      961 ccaggaaggg aagagcgaag gaccttctca ttctctcggg taagcgggtt ccgtgccgcc
     1021 gcgtcagtgc cggaagtgac gccggccgct gaagaggagg gccatccccc gctcgtcggc
     1081 cgcggcgatg acctcggcgt ccttcacgga gcctcccggc tcgacgcacg cggtcacgcc
     1141 cgcgtcggcg aggaggagga ggccgtcggg gaaggggaag aaggcgtcgg aggccgcgca
     1201 ggcccccttc gcgcgcgcgg ctgccttgga gacggcgtcg cccgcggcgt cgacgcggct
     1261 cgtccggccg ccgccgatcc cgtaggtcgc ggtctcgccg ccgacgacga tcgcgttcga
     1321 gatgacgccg gagacggctc tctcgaggaa gaggagcgcg cgcatctcgg cgtcggtcgg
     1381 cgcgcgcttc gtgacgacct tccacgtcgc cgcgtcggcc gggaggacgt ccgcctcctg
     1441 cacgaggacc ccgccgtcga tgccgcgcag ctctcgccgc gcgcgcggcg ccgggtcggg
     1501 ggcgacggtc aggacgcgga ggttcggctt cttcgcgaac gcggcacgcg cctcgtcggt
     1561 gaccccttcg cagacgacga cctcgaggaa cttcgacgtc atcgcctccg cgacggcccg
     1621 gtcgaggacg ccggtgtacg cgaggacgcc gccgaagccc gagaccgggt cggaggccca
     1681 cgccgcctcg aacgaggcgg cggcgctctc ctcgcgcgcg atgccgcacg ggatccggtg
     1741 cttgacgatc gtcatcgccc cgcgcgggag gaggcgcgaa gtgtggagcg ccgagtcggc
     1801 gtcgaggagg ttcgtgtagg agagctcctt cccctggtgg aggacgaagc cggcgagggc
     1861 cgacggcggc gccgccgggt cgcggtagaa ggcggcggcc tggtgggggt tctcgccgta
     1921 gcggagcgtc ccgcccagct cgaacgagag cgtcagccgc tcggcgaaag gagcctcctc
     1981 cgtccgggcc gagagccagg tcgagatcgc cgcgtcgtag gcggcggtcc ggcggaagac
     2041 cttcgccgcg aggcgcttcc tcgtgccgag ggtcgaggcg ccggcgttcg cgtcgagctc
     2101 ggcggcgaac gcggcgtagt cggccgggtc gcagaggacc gcaacgtgcg cgtggttctt
     2161 cgcggcggcg cgcaggagcg agggcccgcc gacgtcgatc ttctcgatcc cctgcgcgaa
     2221 cgtcgtctcg acggccgcga ccgtctcctc gaacgggtag aggttcacga cgacgacgtc
     2281 gatcggcccg acgccgttcg cctccgcctg ctccacgtgc ttcccgaccg aacggtcgta
     2341 gagaatgccg ccgaagagag cggggtggag cgtcttgacg cgcccgtcga agacctcggg
     2401 gaagccggtc acgtcggaga cctgcgtcac ggggaggccg gcgtcggaga ggaccttccc
     2461 ggtgccgccg gtcgaaacga gctcgaagcc gtgccggagg agggcgttcg ccaggtcgag
     2521 gaggccgctg cggtcggaga cggagaggag ggcgcgcttc gtgctcatcg gacggggtac
     2581 ggtcctttcg actcggggcg ggcgagggcg agcgaggcgt cgcctccggc cgcgttccag
     2641 atccaggcga ggaggcgcgt tgcgagcgtc accccggagt cgtacgcctc gcgcgggttg
     2701 tcggtccgga gcggcgcggc gacgagcgtc tccggcgcgt cgaacgggcg ggcggtgaag
     2761 ccgcggaaga ccgaacgctc ggaggcgaag tagacgaggt cgccgtcgaa cggctcggga
     2821 ccgggcggcc cgcagaggag cgccacctcc gcgacgaggc cgcccacctc gaacgcgacg
     2881 tcctcgaagc gccaatggtc gcggatcgcc tgggcgatcc gtcgcgctcc cgacgcgagc
     2941 gcggcgcggc gagcggcggc gtcgcgggcg ggggaggccg cgaggcccgc cttcaccccc
     3001 tccatcaggc gcgtccggtg cttcacgagc tggcgtttca ggtcgggcgg cgcgttcacg
     3061 gcggcgtccg cggacatgac ggcggcggcg ggtaggggcc atcgggcgac ctccgcggcg
     3121 ggcgacggcg cggccgcgag gagcgccagc gggacgagga gggcg
//
LOCUS       DAOTEZ010000484         3116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_24269_length_3116_cov_6.552107, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000484 DAOTEZ010000000
VERSION     DAOTEZ010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24269_length_3116_cov_6.552107"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..1155
                     /locus_tag="P5164_18165"
     CDS             85..1155
                     /locus_tag="P5164_18165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45862.1"
                     /translation="MARLAWYALGPASLSGESPSIAVDPGRKALRFTGLSAEPFELDF
                     DGFDTRSRTALRPEVRREARTATWILLQRAGRLEQAGDPVEAAAVAAEAAAAISPAGD
                     RSLLEWARRCEARLRVAAGQVEDGERRFREIAREPVAAISVAYDAARALHLAGEPSRS
                     IPWYRDAVLGDGRLEEPWLVGDALADAVLALSEVRRFDEALSLVEAAPRLGWSHRQEL
                     RALVLWRAGRPVPPPSGVPPATALRRTWLLEERLASGADPAALLAETVAERRRGSDVD
                     ELLALLEAELLLRTRKAADAWRIASPAFATLWEKRFRDVMVRGHLALAADRAARAAEA
                     AGAPADGLRIRSDVRRFLAAAR"
     gene            1290..2459
                     /gene="sucC"
                     /locus_tag="P5164_18170"
     CDS             1290..2459
                     /gene="sucC"
                     /locus_tag="P5164_18170"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HRY45863.1"
                     /translation="MKVHEYQAKEVLRRYGVTTPKGHVTDSPEEARQIAKELGGKAVV
                     KAQIHAGGRGKGGGVKLAKSPDEAYEHAKAIIGMQLVTHQTGPEGRKVKKVLVEEAAN
                     IAQELYLGITLDRAIGKPVLMASKFGGMEIEEVAHRDPSAILKEPFDAIAGLQPFQAR
                     KVAYALGLTGDTHKKAVPFLLALAKAYVETDCSLAEINPLMITAEGDVVALDAKMNFD
                     DNALFRHLDIKEYRDLDEEDPLEVEASKFNLNYIKLDGNVGCMVNGAGLAMSTMDIIQ
                     YAGGSPANFLDVGGGANEEQVKNAMRIILSDPHVKGVLINIFGGIMRCDIIASGVVKA
                     VKEIGCTVPVVVRLEGTNVEEGKQILRDSGLAVIPADGMKDAAEKIIAAVKKGEN"
     gene            2461..>3116
                     /locus_tag="P5164_18175"
     CDS             2461..>3116
                     /locus_tag="P5164_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HRY45864.1"
                     /translation="MASWLSTDTKVVVQGLTGREGKFHALAGRDYGTQVVAGVTPGKG
                     GTTVEGIPVFDTVHEAVEKTGANASLLFVPPPAAADGIMEAADAGVALVIAITEGIPV
                     NDMIRVKAFLADRPNVRLVGPNCPGVIRPSAKVKLGIMPARIHRPGKVGIVSRSGTLT
                     YEAVGQTTALGLGQSTCVGIGGDPVNGTNFIDCLKMFKDDPETEAIIMIGEIGGSAEE
                     "
BASE COUNT          493 a         1133 c         1097 g          393 t
ORIGIN      
        1 gagcaagacc cgggctccgg agcaagagat cagacggccc ccagtccgcc gacgtccccg
       61 gacctccctc ttttcgatcg ccagatggcg cgcctcgcgt ggtacgcgct cggcccggcc
      121 tcgctttccg gggagtcccc ctcgatcgcg gtcgaccccg ggcggaaagc cctccggttc
      181 acgggactct cggctgagcc attcgagctc gacttcgacg ggttcgacac gaggagtcgg
      241 acggcgctca ggcccgaggt ccggcgggag gcacgaacgg cgacctggat cctcctccag
      301 cgcgccgggc gcctcgagca ggcgggggac ccggtcgaag ccgccgccgt ggccgcggaa
      361 gccgccgccg cgatctcgcc cgcgggagac cggtcgctcc tcgagtgggc gcgccggtgc
      421 gaggcgcgcc tgagggtcgc cgcggggcag gtcgaggacg gcgagcggcg cttccgggag
      481 atcgcgcgcg agccggtggc ggcgatctcc gtcgcctacg acgcggcccg cgccctccac
      541 ctcgccggcg agccgtcgcg gtcgatcccg tggtatcgcg acgccgtcct cggcgacggc
      601 cgcctcgagg agccgtggct cgtcggcgac gcgctcgccg acgcggtcct cgccctctcc
      661 gaggtccgtc gcttcgacga ggccctctcc ctcgtcgagg cggcgcctcg actgggctgg
      721 agccatcggc aggagctccg ggccctcgtc ctctggcggg cggggcgccc ggtcccgccg
      781 ccgtcgggag tcccgccggc gaccgccctt cgccgcacgt ggctcctcga ggagcgcctc
      841 gcgtcgggag cggaccccgc cgcgctcctc gccgagaccg tcgccgagag gaggcgggga
      901 tcggacgtcg acgagctcct cgcgctcctc gaggccgagc tcctgcttcg gacgcgaaag
      961 gccgccgacg cctggcggat cgcctcgccc gccttcgcga ccctctggga gaagcgcttt
     1021 cgcgacgtca tggtccgggg gcacctcgcc ctcgccgcgg accgggccgc gcgggccgcc
     1081 gaggccgcgg gcgccccggc ggacgggctc cgcatccgct ccgacgtccg tcgcttcctc
     1141 gccgcggccc gctgagcggg cgccgcggag agcctcccgg ggcgccctcc cgggcgcccc
     1201 tcggccccgc gatggcccgc tcctggggcg cggactagaa tccgccccgc ccgaccgggc
     1261 gaatcgagat ccgaagaggg gacggacgaa tgaaagtcca cgagtaccag gcgaaggagg
     1321 tcctccgccg ctacggcgtc acgaccccga aggggcacgt caccgacagc ccggaggagg
     1381 cccgtcagat cgccaaggag ctcggcggca aagccgtcgt gaaggcccag atccacgccg
     1441 gcgggcgcgg caaggggggc ggcgtcaagc tcgcgaagag ccccgacgag gcctacgagc
     1501 acgccaaggc gatcatcggg atgcagctcg tcacgcacca gaccggcccc gaggggcgca
     1561 aggtcaagaa ggtgctcgtc gaggaggcgg ccaacatcgc ccaggagctc tacctcggca
     1621 tcacgctcga ccgcgccatc gggaagcccg tcctcatggc ctcgaagttc ggcggcatgg
     1681 agatcgagga ggtcgcccac agggacccgt ccgcgatcct caaggagccg ttcgacgcga
     1741 tcgccggcct tcagccgttt caggcccgca aggtcgccta cgcgctcggc ctgacgggcg
     1801 acacgcacaa gaaggcggtg ccgttcctcc tcgcgctcgc gaaggcctac gtcgagaccg
     1861 actgctccct cgccgagatc aaccccctca tgatcacggc cgaaggcgac gtcgtcgcgc
     1921 tcgacgcgaa gatgaacttc gacgacaacg ccctcttccg ccacctcgac atcaaggagt
     1981 accgggacct cgacgaggag gacccgctcg aggtcgaggc gtcgaagttc aacctgaact
     2041 acatcaagct cgacggcaac gtcggctgca tggtgaacgg ggcgggcctg gcgatgtcga
     2101 cgatggacat catccagtac gccggcggga gccccgcgaa cttcctcgac gtcggcggcg
     2161 gcgccaacga ggagcaggtc aagaacgcga tgcggatcat cctctcggac ccgcacgtga
     2221 agggcgtcct catcaacatc ttcggcggca tcatgcggtg cgacatcatc gcgagcggcg
     2281 tcgtgaaggc ggtgaaggag atcggctgca cggtccccgt cgtcgtccgc ctcgagggga
     2341 cgaacgtcga ggagggcaag cagatcttga gggactccgg cctcgccgtc atcccggccg
     2401 acgggatgaa ggacgccgcc gagaagatca tcgccgcggt gaagaagggg gagaactgag
     2461 atggcctcct ggctgagcac cgacacgaag gtcgtcgtcc agggcctcac ggggcgcgag
     2521 gggaagttcc acgccctggc cggccgtgac tacggcacgc aggtcgtcgc gggcgtcacc
     2581 cccggcaagg gaggcacgac ggtcgagggg atccccgtct tcgacacggt ccacgaggcg
     2641 gtcgagaaga cgggggcgaa cgcctcgctc ctcttcgtcc cgccgccggc cgcggccgac
     2701 gggatcatgg aagccgccga cgccggcgtc gccctcgtca tcgcgatcac ggaagggatc
     2761 ccggtcaacg acatgatccg ggtcaaggcg ttcctcgccg atcggccgaa cgtgaggctc
     2821 gtcggcccga actgccccgg cgtcatccgc ccctcggcga aggtgaagct cggcatcatg
     2881 ccggcgcgca tccaccgccc cggcaaggtc ggcatcgtct cccgctcggg gacgctcacc
     2941 tacgaggcgg tcggccagac gacggcgctc ggcctcggcc agtcgacctg cgtcggcatc
     3001 ggcggcgacc ccgtgaacgg cacgaacttc atcgactgcc tgaagatgtt caaggacgac
     3061 ccggagaccg aggcgatcat catgatcggc gagatcggcg ggagcgccga ggaaga
//
LOCUS       DAOTEZ010000485         3101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_24455_length_3101_cov_4.666776, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000485 DAOTEZ010000000
VERSION     DAOTEZ010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24455_length_3101_cov_4.666776"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="P5164_18180"
     CDS             complement(<1..398)
                     /locus_tag="P5164_18180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HRY45865.1"
                     /translation="MTAERLQKVLAAAGVASRRAAEEMILEGRVTVNGTVVRELGTKA
                     DPAVDHVKVDGKRIRPAASHRYILMNKPRGVVVTRDDPQHRPTVYHLLGTRVSEPVVP
                     VGRLDFDSEGLLLLTNDGALAHRIAHPSGG"
     gene            complement(388..1296)
                     /gene="scpB"
                     /locus_tag="P5164_18185"
     CDS             complement(388..1296)
                     /gene="scpB"
                     /locus_tag="P5164_18185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRY45866.1"
                     /translation="MTDDRDETNPEPTVPEAGPEPSPEAAPTAAEALPEDEPAAAAVP
                     GDAELEEALPLVEALLFASSKPIALDKLAEAAELSEELVARALEALEAACTSAGRGVR
                     LDRVAGGVRLVSRPEYDYPVRRLLGLDGKTKLSMAALETLAIVAYRQPVTGPEIAELR
                     AVNSSSSIRTLLDRKLITTAGRKEVVGTPFLYKTTKEFLVHFGLKGLGDLPKPEELEA
                     IYGLEAPKPDPAQVELFHVDEATGEVEEVGESEPAGASAAEGVPPGPGEEAPAREPAI
                     GGAEPAEEAGPVPGDETARGEERHDG"
     gene            complement(1301..2107)
                     /locus_tag="P5164_18190"
     CDS             complement(1301..2107)
                     /locus_tag="P5164_18190"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HRY45867.1"
                     /translation="MAEGIRIPIPPGAELVQPYPVHLPQFEGPLDLLLHLIRKDEIDL
                     RNIPVADICRQYHEYLVLMQELDLEVAAEFLYVEALLVQIKSQMVLPRNSSVEGGDAP
                     DPREELVNRLLQYRKFKGVAETLHVYEAERIGMHPRPPYRPEAEPEDEETDLSEVSLF
                     DLLTLFKGAVDRFRALHPPAMAIAAQKYSIREKMQEMAGRVRETGRVALTEVFRGLTG
                     RAEAIAVFLAVLELLRLLLVRALQTEEFGEIYLEPTGENLTLDGYEEEYR"
     gene            complement(2172..>3101)
                     /gene="trpS"
                     /locus_tag="P5164_18195"
     CDS             complement(2172..>3101)
                     /gene="trpS"
                     /locus_tag="P5164_18195"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRY45868.1"
                     /translation="NYWGAVKNWVDLQDRYECRYFVADLHALTTDYADTSAIRESTLE
                     AVTDWLSVGLDPEKAVVFVQSQVPQTAELALVFGMFTPLGWLERVPTYKEQLQELREK
                     DLGTYGFLGYPVLMTADIALYRGHYVPVGKDQESHLEICREIVRRFNGLYGEVFPEPQ
                     ALFTETPKVPGLDGRKMSKSYENTIALSDSADDVAKKVMSMVTDPRRARRSDPGNPDE
                     CNLFPFHRLYSSREECAEIDRGCRTAALGCVDCKKWLIRNMNAALEPVRAKRAEITAK
                     PGYVREVLEEGGVKARALATETMKAVSGAMKLL"
BASE COUNT          417 a         1103 c         1071 g          510 t
ORIGIN      
        1 cagccgcccg acgggtgcgc gatccggtgc gcgagcgccc cgtcgttcgt caggaggagg
       61 agaccctccg agtcgaagtc gagccgcccc accgggacga ccggctccga cacgcgcgtc
      121 ccgagcaggt gatagacggt gggccggtgc tgcgggtcgt cccgggtgac gacgacgccc
      181 cggggcttgt tcatcaggat gtagcgatgg gacgcggcgg gccggatcct cttcccgtcc
      241 accttcacgt ggtcgaccgc gggatcggcc ttcgtgccga gctcgcggac caccgtcccg
      301 ttcacggtga cccggccctc gaggatcatc tcctcggccg cgcggcggga cgcgacgccc
      361 gccgcagcga ggaccttctg gagccgttca gccgtcatgg cgctcctctc ctcgtgccgt
      421 ctcgtcgccg ggcacgggtc cggcttcctc ggcgggttct gcgccgccga tcgcgggctc
      481 ccgcgcgggg gcctcttcgc ccggtccggg cggcacccct tccgcggcac tcgcgcccgc
      541 aggctcgctc tccccgactt cctccacctc tccggtcgcc tcgtcgacgt ggaacagctc
      601 cacctgggcc gggtcgggct tcggcgcctc gaggccgtag atcgcctcca gctcctcggg
      661 cttcggaagg tcgccgaggc ccttgagccc gaagtggacg aggaactcct tcgtcgtctt
      721 gtagaggaac ggggtcccga cgacctcttt cctcccggcc gtcgtgatca gcttccggtc
      781 gaggagcgtt cggatcgagg aggaggagtt cacggcgcgc agctcggcga tctccggtcc
      841 ggtcaccggc tgccgatacg ccacgatcgc gagggtctcg agcgccgcca tcgagagctt
      901 cgtcttcccg tccaggccaa ggagccggcg gacggggtaa tcgtactcgg ggcgcgacac
      961 gaggcggacg cctccggcga cccggtcgag ccgcacgccg cgtccggcag aggtgcacgc
     1021 cgcctcgagc gcctcgagcg cccgcgcgac gagctcctcg gaaagctccg cggcctcggc
     1081 cagcttgtcg agcgcgatcg gcttggagga ggcgaagagg agagcctcga cgaggggcag
     1141 ggcttcctcc agctccgcgt cgccggggac ggccgcggcc gccggctcgt cctcgggaag
     1201 agcctccgcc gccgtcggcg ccgcctcggg cgacggctcg ggtcctgctt cgggcacggt
     1261 gggctccgga ttcgtctcgt ctctgtcgtc cgtcatcgtc tcagcggtat tcctcttcgt
     1321 acccgtcgag cgtcaggttc tcgcccgtcg gctcgaggta gatctctccg aactcctccg
     1381 tctggagcgc ccggaccagg aggaggcgga ggagctcgag gacggcgagg aagaccgcga
     1441 tcgcctccgc acgccccgtc agcccccgga agacctccgt cagcgcgacg cgtcccgtct
     1501 cgcggacccg tcccgccatt tcctgcatct tctcccgaat ggaatacttc tgggcggcga
     1561 tcgccatcgc cggcgggtga agcgcccgga agcggtcgac ggctcccttg aagagggtga
     1621 ggagatcgaa gagcgagacc tcggacaggt ccgtctcctc gtcttcgggc tcggcctcgg
     1681 gccggtaagg gggcctcggg tgcatcccga tccgctccgc ctcgtagacg tggagcgtct
     1741 cggcgacccc cttgaacttc cggtactgca gcaggcggtt gacgagctct tcccgcgggt
     1801 cgggagcgtc cccgccttcg accgacgagt tccgcggcag gaccatctgg ctcttgatct
     1861 ggacgaggag cgcctcgacg tagaggaact ccgcggcgac ctcgaggtcc agctcctgca
     1921 tcaggacgag gtactcgtgg tactgccggc agatgtcggc gaccgggatg ttccggaggt
     1981 cgatctcgtc cttgcggatc aggtggagca gcaggtcgag ggggccctcg aactgcggca
     2041 ggtggaccgg gtagggctgg acgagctccg cgccgggggg aatcggaatc cggatgcctt
     2101 cggccacggt gccctctagc gtgccgccgc gcgggacgca ccgggacggc gggtcgggct
     2161 cggcgacacc ctcaaagcag cttcatcgcg ccggaaacgg ccttcatcgt ctcggtcgcc
     2221 agggcgcgcg ccttcacgcc cccttcctcg aggacctctc ggacgtagcc cggcttcgcc
     2281 gtgatctccg cccgcttcgc gcggaccggc tcgagcgcgg cgttcatgtt ccggatcagc
     2341 cacttcttgc agtccacgca gccgagcgcc gccgtccggc agccgcggtc gatctccgcg
     2401 cactcctccc tcgaggagta gaggcggtgg aacgggaaga ggttgcactc gtcggggttg
     2461 cccgggtccg accggcgcgc gcgtcgcggg tcggtcacca tcgacatcac cttcttcgcc
     2521 acgtcgtccg ccgagtcgga gagcgcgatc gtgttctcgt agctcttcga catcttccgg
     2581 ccgtcgaggc cgggcacctt cggagtctcg gtgaagagcg cctgcggctc cgggaagacc
     2641 tccccgtaga ggccgttgaa gcgccgcacg atctcgcggc agatctcgag gtggctctcc
     2701 tggtccttcc ccacggggac gtagtggccg cggtagaggg cgatgtcggc cgtcatcagg
     2761 accgggtagc cgaggaaccc gtaggtgccg aggtccttct cgcgcagctc ctggagctgc
     2821 tccttgtacg tcggcacccg ctcgagccag ccgagggggg tgaacatccc gaagacgagc
     2881 gcgagctccg ccgtctgggg cacctgcgac tgtacgaaga cgaccgcctt ctccgggtcg
     2941 agccccaccg agagccagtc cgtcacggcc tcgagggtcg actcccggat cgccgacgtg
     3001 tcggcgtagt cggtcgtgag ggcgtgcagg tcggccacga agtaccggca ctcgtaccgg
     3061 tcctgcaggt cgacccagtt cttcaccgcg ccccagtagt t
//
LOCUS       DAOTEZ010000486         3079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_24722_length_3079_cov_4.781085, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000486 DAOTEZ010000000
VERSION     DAOTEZ010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24722_length_3079_cov_4.781085"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="P5164_18200"
     CDS             complement(<1..381)
                     /locus_tag="P5164_18200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HRY45869.1"
                     /translation="MSSSVAPDLPRVGMLATVRNRRGVVSAVEPFGSGAKEGLLHLVT
                     LEWADAGGNTEETVVWEREPFARLLEPKALPDVEGEPAMRPDDFDALVRSARWTAMRP
                     FLDPDGGEGPLSRLPVSAPFHGALQ"
     gene            complement(378..2759)
                     /locus_tag="P5164_18205"
     CDS             complement(378..2759)
                     /locus_tag="P5164_18205"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRY45870.1"
                     /translation="MRPDGMSVEEWQRTLRRQYGTSQPFVLKRVGKEKVFSDFLVTNP
                     ASRRTWRVAIRGEEPGVSFCSCPDFATNLLGTCKHVEFVLAALRRRRGAARLLREGFR
                     PAWSSVSLVHGSRRRLRLRLADGAPRGLRKVAVSLVDPDGFIRDEAYGRLEALERAAR
                     RAGHELRVYDDAAQFARTVLEGEERRRRLDMAFPAGAASPAFDGLLKTPLPGYQREGA
                     LFACRAGRAILADEMGLGKTVQAIAAAEIAARWLGVERVLVVCPASLKHQWQDEVERF
                     STRPVAVVGGGRKAREAAWSAPGFLKVTGYDAIHRDLDAIARWAPQLVVLDEAQRIKN
                     WATRVAVAVKRIESPLALVLTGTPLENRLEELISIVQFVDQHRLGPTWRVLHDHQVVD
                     EGSTRVVGYRDLDRLGESLAPVLLRRRKSEVLAQLPERSVKRLFLPLTEAQRELHEEN
                     AEAVAKLVAKWRRHRFLSDADQKRLRIALQAMRMACDDAYLLDGKTRSGRKAAEVDAL
                     LGDLLASEGTKVVVFSQWVRMHELLIERLEAAGRGFAFLHGGVPSGDRGALVRRFRTD
                     PHCRVFLSTDAGGTGLNLQAASAVVNVDLPWNPAVLEQRIGRVHRLGQSRPVQVFDLV
                     AEDSIEQRMLGLLGFKRALFAGVLDGGEKEVRLGGSRLARFLEAVGEVAEPLPGGDPG
                     NERPGKPAPSSEDAAGEASAGGATGASPVPLPAAAPTALPPRADVASLLRAGATLLGQ
                     LAAAAEAASSGAPGGGLQIENDPATGRSVLRLELADAEAFLGALASLAGGRTA"
BASE COUNT          397 a         1149 c         1145 g          388 t
ORIGIN      
        1 ctggagcgcg ccgtggaacg gggccgagac ggggaggcgc gagagcggcc cttcgccgcc
       61 gtccgggtcg aggaacggcc gcatcgccgt ccagcgcgcg gagcggacga gggcgtcgaa
      121 gtcgtcgggc cgcatcgccg gctctccctc gacgtcggga agcgccttcg gctcgaggag
      181 ccgcgcgaac ggctcccgct cccagacgac ggtctcctcg gtgttcccgc cggcgtcggc
      241 ccattcgagc gtgacgagat ggaggagccc ttccttcgca cccgatccga acggctcgac
      301 cgcgctgacg acgccgcgac ggttgcggac cgtcgcgagc atcccgacgc gggggaggtc
      361 cggtgcgacg gacgagctca cgcggtccgg ccccccgcga gagaggcgag agcgccgagg
      421 aaggcctcgg cgtccgcgag ctcgagtcgg aggaccgaac ggccggtcgc cgggtcgttc
      481 tctatctgaa gcccgccccc gggcgcgccg gacgaggccg cctccgcggc ggccgcgagc
      541 tgcccgagga gggtggcgcc ggcgcgcagg agcgacgcga cgtccgcgcg cgggggaagc
      601 gccgtcggtg cggcggccgg gagcgggacc ggggaggctc cggtcgcgcc gcctgccgac
      661 gcctcccccg cggcgtcttc cgacgacggc gccggcttgc cgggccgctc gttcccgggg
      721 tcgccgccgg gcagcggctc cgcgacctcg ccgacggcct cgaggaaacg cgcgaggcgc
      781 gacccgccga ggcggacctc cttctctccc ccgtcgagga cgcccgcgaa gagcgcccgc
      841 ttgaagccga gaagtccgag catcctctgt tcgatcgagt cctccgccac gaggtcgaag
      901 acctggaccg ggcggctctg ccccagcctg tggacccgcc cgatccgctg ctcgaggacg
      961 gcggggttcc aggggaggtc gacgttgacg acggccgagg cggcctgaag gttgaggccg
     1021 gtccccccgg cgtcggtcga gaggaagacc cggcagtgcg ggtcggtccg gaaccgcctc
     1081 acgagggcgc cgcggtcgcc cgacgggacg ccgccgtgga ggaaggcgaa gccgcgcccc
     1141 gcggcctcga gccgctcgat gaggagctcg tgcatccgca cccactggct gaagacgacg
     1201 accttcgtcc cctccgacgc gaggaggtcg ccgaggaggg cgtcgacctc ggcggccttc
     1261 cggccgctcc gggtcttccc gtcgaggagg taggcgtcgt cgcaggccat ccgcatcgcc
     1321 tggagcgcga tccggaggcg cttctggtcg gcgtcggaga ggaaccggtg ccgccgccac
     1381 ttcgcgacga gcttcgcgac ggcctcggcg ttctcctcgt gcagctcccg ctgggcctcg
     1441 gtgagcggca ggaagagcct cttgacgctc cgctcgggga gctgcgcgag gacctcggac
     1501 ttccgcctcc ggaggaggac cggcgcgagg ctctcgccga ggcggtcgag gtcgcggtac
     1561 cccacgaccc gcgtcgagcc ctcgtcgacg acctggtggt cgtggagcac ccgccacgtc
     1621 ggcccgagcc ggtgctggtc gacgaactgg acgatcgaga tcagctcctc gagccggttc
     1681 tcgagcggcg tccccgtcag gacgagcgcg agcggcgact cgatccgctt gacggccacg
     1741 gcgacgcgcg tcgcccagtt cttgatccgc tgcgcctcgt cgaggacgac gagctgcggc
     1801 gcccaccggg cgatggcgtc gaggtcccgg tggatcgcgt cgtagcccgt gaccttcagg
     1861 aagcccgggg cgctccaggc cgcctcgcgc gccttccgcc cgccgccgac cacggcgacc
     1921 ggccgcgtgg agaagcgctc gacctcgtcc tgccactggt gcttgagcga ggccgggcag
     1981 acgacgagga cgcgctcgac gccgagccac cgggcggcga tctcggcggc ggcgatcgcc
     2041 tgcaccgtct tgccgaggcc catctcgtcg gcgaggatcg cccgccccgc gcggcaggcg
     2101 aagagcgccc cctcgcgctg gtagcccggc agcggcgtct tcaggagccc gtcgaaggcg
     2161 gggctcgcgg cgcccgccgg gaaggccatg tcgagccggc ggcgccgctc ctcgccctcg
     2221 aggaccgtgc gcgcgaactg ggccgcgtcg tcgtagaccc tcagctcgtg cccggcacgc
     2281 cgggcggctc tctcgagcgc ctcgaggcgc ccgtacgctt cgtcgcggat gaagccgtcc
     2341 gggtcgacga ggctcaccgc gaccttccgg aggccgcgcg gcgcgccgtc ggcgagccgg
     2401 agcctcagcc gacgccggga gccgtggacg agcgagacac tgctccaggc gggacgaaac
     2461 ccctcgcgga ggaggcgcgc cgcgccccgc cgccttcgga gcgcggcgag gacgaactcc
     2521 acgtgcttgc acgtgccgag gaggttcgtg gcgaagtcgg ggcacgagca gaagctgacg
     2581 cccggctcct cgccgcggat cgcgacccgc caggtcctcc ggctcgccgg gtttgtgacg
     2641 aggaagtcgg agaagacctt ctccttcccg accctcttca ggacgaacgg ctgcgaggtc
     2701 ccatactgcc ggcgcagggt gcgctgccat tcctcgaccg acatcccgtc ggggcgcacg
     2761 tggcgggaga ctctcggcgg cgacgcggct ccccgcgcgg cggcgctcgc cgtccgccgg
     2821 gctccggacc ggctctcccc tgtcccccgc ctccgatccg ccatcgctcg cctccgcgtg
     2881 gggaggctac acgagcccct gccattcagg gaccgcggtt cggataccgg ggctccctgg
     2941 cctacgccgc ctgctccgcg gcccggtcgc tgacgatccg gccgtcgcgg atctcgacga
     3001 tccggctcgc gcggcgggcg agcaccatgt cgtgcgtgat gacgacgagc gtcgagccct
     3061 gcttccagag gtcgaggaa
//
LOCUS       DAOTEZ010000487         3056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_24990_length_3056_cov_5.251250, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000487 DAOTEZ010000000
VERSION     DAOTEZ010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24990_length_3056_cov_5.251250"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="P5164_18210"
     CDS             <1..249
                     /locus_tag="P5164_18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007476059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRY45871.1"
                     /translation="GAKAVITGDITQIDLPAGRVSGLKEAGRILSGVEEIRFIRFDHR
                     DVVRHPIVQAIVNAYDTFEGGKPAPDVLGRVRAASEEA"
     gene            256..747
                     /gene="ybeY"
                     /locus_tag="P5164_18215"
     CDS             256..747
                     /gene="ybeY"
                     /locus_tag="P5164_18215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRY45872.1"
                     /translation="MGKEPPARRADRVPRATPLVEVTSTVRAGPSVRRVAAWTHAVLA
                     AAAPRRAATVSVLLCGDGRMRRLNREFRRIDRPTDVLSFPADGPGFLGDVAIDVPYAG
                     RQARKRGHALDREVQLLLAHGVLHLLGHDHETDDGEMFRLQRRLVARAFGPGPDGVPG
                     DEA"
     gene            744..2018
                     /locus_tag="P5164_18220"
     CDS             744..2018
                     /locus_tag="P5164_18220"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRY45873.1"
                     /translation="MTDFAIGTGLAFFLSAAFVLLYAILSAFAHALESLSAIRRRSLL
                     EGQEAKYGRLLARENVLISRVAVRLTAQGAVLAGLLSLGTGLSGLSVPEPFLVSAVSI
                     LLGWFVVETLVIRAVARRGAEDLLHDFSWLIPVVLLFATPLYPLLSRLVHPADEDEDE
                     EPVSAAEKEAEKDADVRALLDVAREEGILEKHEEELVSRAVDFGDRTVGEVMTSRPDM
                     VVAEADLPFSAVADLFERTKHTRIPLVEGGVEKPIGVVHVKDVFSALRAPVPPVNARP
                     LARPVLFVPETQTITTLLSDFRRRRQHLAIVVDEWGAVAGVVTLEDLLEELVGEIADE
                     HEDSIDPVIPLAEGAFTVAGRVRVTEIAQLFDVDLAPGDYDTVAGLLSARLGHIPRPG
                     EEVEEGGLLFVVEDADRKRVHRVKVARPRGIA"
     gene            2015..2899
                     /gene="era"
                     /locus_tag="P5164_18225"
     CDS             2015..2899
                     /gene="era"
                     /locus_tag="P5164_18225"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRY45874.1"
                     /translation="MSRRAGTVAVAGRPNAGKSTLVNALVGTKVAIVSDKPQTTRRRV
                     LGMLDRPEGQIVFVDTPGLHKPLHRLNRVMLDEAMEAILDVDLVLLVIDAAEAEGAGD
                     RHALELVGKAKPPRIAVLNKIDLVSKPKLLPRMAVLSATGLFDEIVPVSAATGDGLEG
                     LPELLLRYLPEGDDLYPAGTVTTSDDRTRVAELIREKFLERTREEIPYGLGVVVEEWV
                     VDEAKGMTRVTATLVVDKESHKRIVIGLGGQLIRDAGTAARLEIEKTFGRRFFLDLHV
                     VARPGWREDPRFLGTLSS"
BASE COUNT          412 a         1134 c         1080 g          430 t
ORIGIN      
        1 ggcgcgaagg cggtcatcac gggggacatc acccagatcg acctccccgc gggtcgggtc
       61 tccgggctca aggaggcggg gcggatactc tccggcgtcg aggagatccg cttcatccgc
      121 ttcgaccacc gcgacgtcgt ccggcacccg atcgtccagg ccatcgtgaa cgcctacgac
      181 accttcgagg gcgggaagcc ggctcccgac gtcctcggcc gggttcgcgc cgcctccgag
      241 gaggcgtaga cggccgtggg caaagagcct ccagcgcggc gcgccgaccg cgtcccccga
      301 gccacgcccc tcgtcgaggt gacctccacg gtccgcgcgg gaccgtccgt ccggcgggtc
      361 gccgcgtgga cgcatgccgt actcgcggct gccgcgccgc gtcgggcggc gaccgtctcc
      421 gtcctgctct gcggcgacgg gcggatgcgc cggctgaacc gcgagttccg gcggatcgac
      481 cgacccaccg acgtcctctc gttcccggcg gacggccccg gcttcctcgg agacgtggcg
      541 atcgacgtcc catacgccgg ccggcaggcg cgcaagcgag gccacgcgct cgaccgcgag
      601 gtgcagctcc tcctggcgca cggggtcctc cacctcctcg gtcacgacca cgagacggac
      661 gacggcgaga tgttccgtct ccagcggcgc ctcgtggcgc gggccttcgg ccccggcccc
      721 gacggagtcc cgggggacga ggcgtgaccg acttcgccat cggaacgggc ctcgcgttct
      781 tcctctcggc cgcgttcgtc ctcctctacg cgatcctctc ggccttcgct cacgcgctcg
      841 agagcctctc cgccatccgg cgccggtccc tcctcgaagg gcaggaggcg aagtacggcc
      901 ggctcctggc ccgcgagaac gtcctgatct cgcgcgtggc ggtccggctc acggcgcagg
      961 gggcggtcct cgcgggcctc ctttcgctcg gcacgggcct ctccggcctc tccgtgccgg
     1021 agccgttcct cgtctcggcg gtctccatcc tcctcggctg gttcgtcgtc gagacgctcg
     1081 tcatccgcgc cgtcgcccgc cgcggggccg aggacctgct ccacgacttc tcgtggctca
     1141 tcccggtcgt cctcctcttc gcgacgccgc tctacccgct cctctcgcgc ctcgtccacc
     1201 cggccgacga ggacgaggac gaggagccgg tctccgcggc cgagaaggag gccgagaagg
     1261 acgccgacgt ccgcgccctc ctcgacgtcg cacgcgagga agggatcctc gagaagcacg
     1321 aggaggagct cgtctcccgg gcggtcgact tcggcgaccg gaccgtgggc gaggtcatga
     1381 cgtcgcgccc ggacatggtc gtggccgagg cggacctccc gttctccgcg gtcgccgacc
     1441 tcttcgagcg gacgaagcac acccgcatcc cgctcgtgga gggaggcgtc gagaagccga
     1501 tcggcgtcgt ccacgtgaag gacgtcttct cggccctccg ggcgcccgtc cccccggtca
     1561 acgcgcggcc gctcgcgcgg ccggtcctct tcgtcccgga gacgcagacg atcacgacgc
     1621 tcctctccga cttccggcgc cgccgccagc acctggcgat cgtcgtcgac gagtggggcg
     1681 cggtcgcggg cgtcgtcacg ctggaagacc ttctggagga gctggtggga gagatcgccg
     1741 acgagcacga ggactccatc gaccccgtca tcccgctcgc ggagggcgcc ttcacggtgg
     1801 ccggccgggt ccgggtcacc gagatcgccc agctcttcga cgtcgacctc gcgccgggcg
     1861 actacgacac cgtcgcgggg ctcctctcgg cgaggctcgg gcacatcccg cggcccggcg
     1921 aggaggtcga ggaggggggg ctcctcttcg tcgtggagga cgccgaccgc aagagggtcc
     1981 accgggtgaa ggtggcgcgc ccgaggggga tcgcatgagc cggcgcgccg ggaccgtcgc
     2041 cgtcgcgggg cggccgaacg cggggaagtc gacgctcgtg aacgccctcg tcgggacgaa
     2101 agtcgcgatc gtctccgaca agccgcagac gacgcggcgg cgcgtcctcg ggatgctcga
     2161 ccgccccgag gggcagatcg tcttcgtcga cacgccgggc ctgcacaagc cgctccaccg
     2221 cctgaaccgg gtgatgctcg acgaggcgat ggaggcgatc ctggacgtcg acctcgtcct
     2281 cctcgtcatc gacgccgccg aggcggaagg cgcgggcgac cgccatgccc tcgagctcgt
     2341 cggaaaggcg aagcctcccc ggatcgccgt cctcaacaag atcgacctcg tctccaagcc
     2401 gaagcttctc ccccggatgg cggtcctctc cgcgacgggg ctgttcgacg agatcgtccc
     2461 ggtctccgcg gcgaccggcg acgggctgga aggccttccg gagctcctcc tgcgatacct
     2521 tccggaaggg gacgacctct atccggcggg caccgtcacg acctccgacg accggacgcg
     2581 cgtggccgag ctgatccggg agaagttcct ggagcggacg cgcgaggaga tcccgtacgg
     2641 tctcggcgtc gtcgtcgagg agtgggtcgt cgacgaggcg aaggggatga cgcgcgtgac
     2701 ggcgacgctc gtcgtcgaca aggagagcca caagcggatc gtgatcggcc tcggcgggca
     2761 gctgatccgc gatgccggca cggcggcccg gctcgagatc gagaagacgt tcggccggag
     2821 gttcttcctc gacctgcacg tcgtcgcccg gccgggctgg cgggaggacc cgcgcttcct
     2881 cgggaccctc tccagctgac ggccgcaccc ccgaaggcgg cccggaaggg ccggcgctcg
     2941 gggagagcgg ccggcgaagg accggccggc gaaggaccgg ccggcggccg atcttcccgt
     3001 cctggcttga cgccgcgcgg cgaacgccgg atcgttcccg gtgcggagga atccgt
//
LOCUS       DAOTEZ010000488         3031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_25293_length_3031_cov_4.885753, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000488 DAOTEZ010000000
VERSION     DAOTEZ010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25293_length_3031_cov_4.885753"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2287)
                     /locus_tag="P5164_18230"
     CDS             complement(<1..2287)
                     /locus_tag="P5164_18230"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HRY45875.1"
                     /translation="MTRAALVAASLVLLGTAPPPKATPLGGVGWTAAPAPGVFLRVSP
                     AVGEAVRLDFDFGGKAGWAAATRKVDLVLPERWEIRMRLRGEALPNDLEVKLADPSGE
                     NVWWFRKRNATLPAAFEPLRVKQRQVEFAWGPAGGGAPSRIGSIEVTVTAGKGGKGWL
                     EVADLVLLELPPEPPAGPPSQAASSSAPGHDAALAADGDPATSWRAAKSGPAEWRVDL
                     GALRELGGLVVEHGASFASRYVVDLSEDGATWRTVREVAGGNGGRDLLALPESVARAI
                     RLRLLEGPRPGPFELKEVSVKPLAFAATPNAFLAEVAKESPRGAWPRYLAGEQSYWTV
                     VGVDGDPDEGLLSEDGALEAGSGLFSIEPFLVAGGRLLTWADVTARHALVDGDLPIPS
                     VTWDAGETRLETTALATGLPGASALLARYRVANLTKEERRVRLVLAIRPLQVNPPWQF
                     LGTPGGASEIRSLAFEGARALAFEGARALVNGERVVAALVPPSAAGATTLDAGDVSDH
                     LRAGGLPPAASASDPAGFASGAFAFDLVVPAGGSREVVLATPFDMSRVPTELAAALGA
                     EPWRGLPPADSSALFESLLARERSAWRSRLDRVAFDVPPAAERWVKVLRSQLAFVLVN
                     RDGPAIQPGSRSYARSWIRDGSLTSEALLRLGQGGVARSFLMWFSGYHGEDGRVPCCV
                     DARGADGVPENDSHGQLLFLIGETVRYTRDRGLAARMWPNVVSAVAYVDALRQRRRTP
                     EYETPEKRVFYGLLPESISHEG"
     gene            complement(2284..>3031)
                     /locus_tag="P5164_18235"
     CDS             complement(2284..>3031)
                     /locus_tag="P5164_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRY45876.1"
                     /translation="LWMLSASFMTTGEANASPPPLLPAKATLAQYRELFVRLDLGRSF
                     VNSSILALCAALLSVVVNAAAGYAFAKLRFPGRGRLFAGLLAAMVIPGQVAMLPVFLL
                     LRSVGLVNSFAGVILPGVASIFGIFLVRQFASSVPDELLDAARIDGAGELRIFWSIVL
                     PLCRPILVTLGVLTFLGTWNDFMWPLIVLTDDAKWTLTVSLASLAGEHVQDTELMMAG
                     AVVTVLPALLLFLFLQRSYIDGITAGSIKE"
BASE COUNT          464 a         1123 c         1112 g          332 t
ORIGIN      
        1 acccctcgtg gctgatcgac tcggggagga ggccgtagaa gacccgcttc tccggcgtct
       61 cgtactccgg cgtccgccgc ctctgccgga gcgcgtcgac gtacgcgacg gcggagacga
      121 cgttcggcca catccgcgcc gcgagccccc ggtcgcgcgt gtatcggacc gtctctccga
      181 tcaggaagag gagctgcccg tggctgtcgt tctcggggac gccgtcggcg ccgcgcgcgt
      241 cgacgcagca tgggacgcgc ccgtcctcgc cgtggtagcc ggagaaccac atgaggaacg
      301 agcgtgcgac tcccccctgc ccgagcctga ggagcgcctc cgacgtcagc gagccgtccc
      361 ggatccagga acgggcgtag gagcgcgatc cgggctggat cgccggcccg tcgcggttca
      421 cgaggacgaa cgcgagctgg gagcggagga ccttcaccca ccgctccgcc gcgggcggca
      481 cgtcgaaggc gacccggtcg aggcggctcc tccaggcgct ccgctcgcgc gcgaggagcg
      541 actcgaagag ggccgaggaa tcggcgggcg ggaggccgcg ccacggctcc gccccgagag
      601 ccgccgcgag ctccgtcggg acgcgcgaca tgtcgaacgg agtcgcgagg acgacctcgc
      661 gcgaacctcc cgccgggacg acgagatcga aggcgaacgc gcccgaggcg aagccggccg
      721 gatcgctcgc cgacgccgcc ggagggagcc ccccggcgcg gagatggtcg gagacgtctc
      781 ccgcgtcgag cgtcgtcgcc ccggccgccg acggcggcac gagcgcggcg acgacccgct
      841 cgccgttcac gagggcgcgc gctccctcga acgcgagggc gcgcgctccc tcgaacgcga
      901 gggagcgaat ctcggaggct ccgcccggcg ttccgaggaa ctgccagggc gggttcacct
      961 ggagcggccg gatcgcgagg acgagccgga ccctccgctc ctccttcgtc aggttcgcga
     1021 cccggtagcg cgcgaggagg gcggaagcgc ccgggagccc cgtcgcgaga gcggtcgtct
     1081 cgaggcgcgt ctcccccgcg tcccacgtta ccgaggggat cgggaggtcg ccgtcgacga
     1141 gggcgtgccg cgccgtcacg tcggcccagg taaggagccg gcctccggcc accaggaacg
     1201 gctcgatcga gaagagaccg gagcccgcct cgagcgcgcc gtcctccgag aggagcccct
     1261 cgtccgggtc gccgtcgacg ccgacgacgg tccagtacga ctgctcgccg gcgaggtacc
     1321 gcggccaggc accgcgcggc gactccttcg cgacctcggc gaggaacgcg ttcggcgtcg
     1381 ccgcgaaggc gagcggcttc accgagacct ccttcagctc gaacggcccc ggccgcggcc
     1441 cctcgaggag gcgaagacgg atcgcccgcg ccacgctctc ggggagcgcg aggaggtccc
     1501 gcccgccgtt cccgcctgcg acctcgcgaa ccgtccgcca cgtggcgccg tcttccgaga
     1561 gatccacgac gtagcggctc gcaaacgacg cgccgtgctc gacgacgagg ccgcccagct
     1621 cgcgaagcgc tccgaggtcg acccgccact cggccgggcc ggacttcgcc gcgcgccacg
     1681 acgtcgccgg gtcgccgtcc gccgcgagcg cggcgtcgtg ccccggggcg gacgaggagg
     1741 cggcctggct cggaggcccg gcgggcggct cgggcgggag ctcgaggagg acgaggtcgg
     1801 ccacctcgag ccaccccttc ccccctttcc cggccgtcac cgtcacctcg atcgacccga
     1861 tccgcgacgg cgccccgccc cccgcggggc cccacgcgaa ctccacctgc cgctgcttca
     1921 cgcggagcgg ctcgaaggcc gccgggagcg tcgcgttccg cttccggaac caccagacgt
     1981 tctcgcccga ggggtcggcg agcttcacct cgaggtcgtt ggggagcgcc tcgccgcgga
     2041 ggcgcatccg gatctcccac ctctccggga ggacgaggtc gaccttccgc gtcgccgcgg
     2101 cccaccccgc cttccccccg aagtcgaagt cgaggcgaac ggcctcgccg accgccggcg
     2161 agacccgcag gaagacgccc ggcgccggcg cggcggtcca gcccaccccg ccgagaggcg
     2221 tcgccttcgg cggcggcgcg gtcccgagga gaacgagcga ggcggcgacg agggcggctc
     2281 gcgtcattcc ttgatgctcc cggccgtgat gccgtcgatg tacgaacgct ggaggaacag
     2341 gaagaggagg agcgcgggga ggaccgtgac gaccgccccg gccatcatca gctccgtgtc
     2401 ctggacgtgc tcccccgcga ggctcgcgag ggagaccgtc agcgtccact tcgcgtcgtc
     2461 cgtcaggacg atgagcggcc acatgaagtc gttccaggtg ccgaggaacg tcaggacgcc
     2521 gagcgtgacg aggatcggcc ggcagagcgg gaggacgatg gaccagaaga tccggagctc
     2581 ccccgccccg tcgatccgag ccgcgtcgag gagctcgtcc gggaccgagg aggcgaactg
     2641 ccggacgagg aagatcccga agatgctcgc gactcccgga aggatcaccc cggcgaagga
     2701 gttgacgagc cccaccgacc gcagcaggag gaagaccggg agcatcgcga cctggcccgg
     2761 gatcaccatc gccgcgagga gccccgcgaa gaggcgcccc cgccccggga agcggagctt
     2821 ggcgaacgcg tagccggcgg cggcgttcac gacgaccgag aggagcgccg cgcagagggc
     2881 gaggatcgag ctgttcacga agctccgccc gaggtcgagc ctcacgaaga gctcgcggta
     2941 ctgcgcgagc gtcgccttcg ccgggaggag cggcggcggg gaggcgttcg cttcgcccgt
     3001 cgtcatgaac gaggccgaca gcatccagag g
//
LOCUS       DAOTEZ010000489         3017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_25471_length_3017_cov_6.471641, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000489 DAOTEZ010000000
VERSION     DAOTEZ010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25471_length_3017_cov_6.471641"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..2226
                     /gene="pheT"
                     /locus_tag="P5164_18240"
     CDS             160..2226
                     /gene="pheT"
                     /locus_tag="P5164_18240"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRY45877.1"
                     /translation="MKFRLEWLGQYLPGDLPDLATLRRRLTDVGFIVEGTEGEGKDAV
                     LEVELTANRPDGMNHRGLAREAACALGRAFRDVPAPAPPEGPAAASSLAAVTIEAPDL
                     CSRYSARVVEGLRVREASAAVQERLRGLGMNPISAPVDATNHVLWDIGQPLHAFDLDT
                     LAKKADGLPAIVVRRARSGETLVTLDGVERTLSPEDLVIADAEKPVALAGVMGGLATA
                     ITPSTTRVLLESAHFTATAVRKTARRHGMHTDASHRFERGTDPETTLEALDRVTALLL
                     ADCGGTVARGVIDAHGTPRPRKSVTLRHARLVSFLGMEVPLERCVEILSSLAFGVREA
                     APGVLEVSVPTWRVDVEQECDLIEEVIRVVGYAALPETLPHPYNPTWSEPVLAREERA
                     RDLLAAAGFAEACTYSFVSAEENSPFASCVDGTPPVVSNALGEPFTTMRSTPVVGLLR
                     AARHNVRRGLGDLALFEVGRSYALVEGRPVESRRAAILLRGRRGVHWASEARPADFHD
                     GSGVVAALFAGFGAPAPAFRPAALPFLAPGRSAEVVVDGKVVGWVGVLSGTLAAEWDL
                     GEVVAADVDLAAIPVPPPPSSVEMPSRFPGSEVDLTVTHPFSLSWDTLAAAVRAGAPS
                     ELLSVDAKYLWRGAGVPEGHVKTTLTVAFGSAERSLERDEINRWRDDAARRLLAVPGT
                     AVDGIA"
     gene            2238..2576
                     /locus_tag="P5164_18245"
     CDS             2238..2576
                     /locus_tag="P5164_18245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45878.1"
                     /translation="MSGKKDKAPLEETAPEAVSELEQLLGTMEEKVARLLSAAKESAG
                     ENARLRAALEETAAERDRLRAELTDSREGAVHVAELNEKLSRLATEREDVRARIERLV
                     KSLEEPAAIA"
     gene            2627..2983
                     /locus_tag="P5164_18250"
     CDS             2627..2983
                     /locus_tag="P5164_18250"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRY45879.1"
                     /translation="MENRPTVIQVEIFGQTYSVRAAGEEEYIRDLAAFVDRKMREVAA
                     HAPTVDSTKIAILAALNVSDEFHQYRTKTRMGDPGRLVPVAGRLVEKLDEILGILPAE
                     LPADAGTEWRGTLRGA"
BASE COUNT          415 a         1091 c         1089 g          422 t
ORIGIN      
        1 cacggcatcc ccgacctgcg cctcctcttc gagaacgacg tccggttcct cgaccggacg
       61 tggggctgag cgtgcgctct gcgcacgacg agctgaccgt gcgctccgcg cgcggcgaac
      121 acgggagccg cggtcgcggc ttcccgggga gctgacccga tgaagttccg cctcgagtgg
      181 ctcgggcagt acctgcccgg cgacctcccc gacctcgcca cgctccgccg ccgcctcacg
      241 gacgtcggct tcatcgtgga gggaacggaa ggggagggga aggacgcggt cctcgaggtc
      301 gagctgacgg cgaaccgccc cgacgggatg aaccaccgcg ggctggcccg cgaggccgcc
      361 tgcgcgctcg gacgggcgtt ccgcgacgtc cctgcgcccg cgccgccgga gggtccggcg
      421 gcggcgtcct ccctcgcggc ggtgacgatc gaggcccccg acctctgctc gcgctactcc
      481 gcgcgcgtcg tggaagggct gcgcgtgcgg gaggcgtcgg cggcggtgca ggaacgcctt
      541 cggggcctcg gcatgaatcc gatctcggcg ccggtcgacg cgacgaacca cgtcctctgg
      601 gacatcggcc agcccctcca cgccttcgac ctcgacacgc tcgcgaagaa agccgacgga
      661 ctccccgcga tcgtcgtccg ccgcgcccgc tccggggaga cgctcgtcac cctcgacggc
      721 gtggaacgga cgctctcgcc ggaggacctg gtcatcgcgg acgccgagaa gcccgtcgcc
      781 ctcgcgggcg tcatgggcgg cctcgcgacg gcgatcacgc cctccacgac gcgcgtcctc
      841 ctcgagtcgg cgcacttcac ggccaccgcg gtccggaaga cggcccgccg gcacgggatg
      901 cacacggacg cctcccaccg cttcgagcgg gggaccgacc cggagacgac gctcgaagcg
      961 ctcgaccgcg tcacggcgct cctcctcgcc gactgcggcg ggacggtcgc gcgcggggtg
     1021 atcgacgcgc acgggacgcc gcggccgcgg aagagcgtga ccctccggca cgcgcgcctc
     1081 gtctcgttcc tcgggatgga ggtgccgctc gagcgctgcg tcgagatcct ttcctccctc
     1141 gcgttcggcg tgagagaggc ggcgcccgga gtcctcgagg tctccgtgcc gacctggcgg
     1201 gtcgacgtcg agcaggagtg cgacctgatc gaggaggtca tccgggtcgt cggctacgcg
     1261 gcgctcccgg agacgctccc gcacccgtac aacccgacct ggtccgagcc ggtcctcgcc
     1321 cgcgaggagc gcgcccggga cctcctcgcc gcggcgggct tcgccgaggc gtgcacctac
     1381 tcgttcgtct cggccgagga gaactctcct ttcgcgtcct gcgtcgacgg gacgcccccg
     1441 gtcgtctcga acgcgctcgg ggagcccttc acgacgatgc gctcgactcc tgtggtcggg
     1501 ctgctccgcg ccgcccggca caacgtccgg cgcggcctcg gcgacctcgc cctcttcgag
     1561 gtcgggcgct cctacgcgct cgtcgaagga cgtccggtcg agtcccggcg tgcggcgatc
     1621 ctcctgcgag gccgacgggg cgtccactgg gcgtccgagg cgcgccctgc cgacttccac
     1681 gacggttcgg gcgtcgtggc cgccctcttt gccggcttcg gcgcgcccgc accggcgttc
     1741 cgaccggcgg cgctcccgtt cctcgccccg ggccggtcgg ccgaggtcgt tgtggacggg
     1801 aaagtcgtcg gctgggtcgg ggtcctctcc gggacgctcg ccgccgagtg ggacctcggg
     1861 gaggtcgtcg cggccgacgt cgacctcgcg gcgatcccgg tcccaccccc cccttcgtcc
     1921 gtggagatgc cgtcgcgctt ccccgggagc gaggtggacc tgacggtgac ccacccgttc
     1981 tcgctttcct gggacacgct cgccgcggcc gttcgggccg gcgccccgtc cgagctcctc
     2041 tcggtcgacg cgaagtacct gtggcgcggc gcgggcgtcc ccgaggggca cgtcaagacc
     2101 accctcaccg tggccttcgg gtccgcggag cgctccctgg agcgcgacga gatcaatcgc
     2161 tggcgcgacg acgccgcccg gcgcctcctc gccgtccccg ggacggcggt ggacggaatc
     2221 gcctgaagga gaaccagatg agcgggaaga aggacaaggc tcctctcgaa gagacggcac
     2281 cggaggccgt ctcggagctg gagcagctgc ttggaacgat ggaggagaag gtggcccggc
     2341 tcctctcggc ggcgaaggag tccgcagggg agaacgcccg gctgcgtgcc gcgctcgagg
     2401 agacggcggc cgagcgggac cggctccggg ccgagctcac ggacagccgg gaaggggccg
     2461 tgcacgtggc cgagctgaac gagaagcttt cgcgcctcgc gacggagcgg gaggacgtcc
     2521 gagcccgtat cgagcgtctg gtcaagagct tggaggagcc ggccgcgatc gcctgagtcg
     2581 gcgcgtgcta tcctcggaac ggcagcagag aaggagagct gccgggatgg agaacaggcc
     2641 gaccgtcatc caggtggaga tcttcgggca gacctattcg gtccgcgcgg cgggcgagga
     2701 ggagtacatc cgtgacctcg cggcgttcgt ggaccggaag atgagggagg tcgcggcgca
     2761 cgccccgacc gtcgactcga cgaagatcgc catccttgcg gccctgaacg tctccgacga
     2821 attccatcag taccgaacga agacccggat gggtgatccg gggcggctcg tcccggtggc
     2881 gggccgtctc gtcgagaaat tggatgagat cctcggaatc ctgcctgcgg agctcccggc
     2941 ggacgccgga acggaatggc gtggcaccct gcgcggtgcg tgaatgagca catgtccttg
     3001 aaccgatatt tgaaccc
//
LOCUS       DAOTEZ010000490         3015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_25501_length_3015_cov_5.291554, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000490 DAOTEZ010000000
VERSION     DAOTEZ010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25501_length_3015_cov_5.291554"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="P5164_18255"
     CDS             <1..608
                     /locus_tag="P5164_18255"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY45880.1"
                     /translation="RMVGAWERTGGIIVGAIANHGDRWLDTGIHICKFSKVLPGGAPR
                     PVDNAPTANAMVDRATWERVGPIPEDVLQGDASWSRRALQRGVQLTFAPDAVVEHHHL
                     ETARGFLRERHRRGLELGTLRCEWERGHPLRLALVAAATVVPVRALRIGALVTGQCAR
                     GGGLGALAVCSPIVALGHLSSLAGEARAYVRAALGARSRPR"
     gene            complement(735..2675)
                     /locus_tag="P5164_18260"
     CDS             complement(735..2675)
                     /locus_tag="P5164_18260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45881.1"
                     /translation="MRANRFLLAGLLPALLLAGAASAQDVPLDVEIGYRFTDIKGNEE
                     MFKSQLDEEDGLQLRSLSWGLGDIRDTNFVDHFRIDAADLGAGPNGLLRVETGRTGYW
                     KLNASWRRSERYSNLPTIANPFLGNGNLDSQHSYDRTRNSVDVDLELLPGKKIRPLLG
                     FSYSRYSGPGLTTYHVGQDDFQLQQDLKERDTEYRVGVAFDAGPVSGQVVQGWRTFKS
                     DENLKLLAGSGNESGTILGVPVSLDSLTRRSKAEFDTPVTSAVVSGKLGSAIRLTGTY
                     VRADADGDDTSDETLSGNLVSYEILRYFKGLAESSAATAEALSWRGSIRADVHLAAGF
                     DLSTGFTRRNRELEGYSLVNDLWGTTTTFSGFDPRDVSTMLEARTRLERNEDIFDIRA
                     SLKLGKAVTLRLGFVDNRTDVAVENDEAEVVVPGNQGGTYDRCIQGMEGGLAFKLGGL
                     SLGVDYIREQADAAVVRTDFRERDRLRLRGAWQAAKWLRLGGNAEIVDTSNDDFGYGF
                     EGDMNTYGGDVEVSPWEFLTFRVGAGRFEGDHSIAYRVPQTWADADSVYRERGDSLEG
                     GLTLNLKPFTLEALYRTFENDGSFAYELDRARLRAAWDFTELFGIAAEWDLDDYAETA
                     RTYGTGADFKANRYGLYLRIRK"
     gene            complement(2688..>3015)
                     /locus_tag="P5164_18265"
     CDS             complement(2688..>3015)
                     /locus_tag="P5164_18265"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRY45882.1"
                     /translation="LTRQGEAPCLTCHTEKRGPFVYEHVGPTVGGMTGGCLSCHEPHG
                     SNNPKQLTRSRVDLLCLECHSTLAAGALGSQPPATHNISLPRWRNCTTCHVAVHGSNR
                     SPKLLK"
BASE COUNT          415 a          996 c         1139 g          465 t
ORIGIN      
        1 agcggatggt cggcgcctgg gagcgcaccg ggggaatcat cgtcggagcg atcgcgaatc
       61 acggggaccg atggctcgat accgggatcc acatctgcaa gttctcgaag gtcctccccg
      121 gcggcgcgcc tcgacccgtc gacaacgccc cgacggcgaa cgcaatggtg gaccgcgcca
      181 cctgggagag ggtcggcccg atcccggagg acgttctgca gggcgacgcg agctggagcc
      241 gtcgcgcgct ccagcgcggc gtgcaactca cgttcgcccc ggacgcggtc gtcgagcacc
      301 accacctcga gaccgcccgc ggcttcctcc gggagcgcca tcgccggggc ctcgagctcg
      361 gcacgctccg ctgcgaatgg gaacggggcc accccctccg cctcgccctc gtggcggccg
      421 caaccgtggt gcccgtgcgc gcgctccgga tcggtgcgct cgtcaccggg cagtgtgcgc
      481 gcggaggagg cctcggagct ctcgcggtct gcagcccgat cgtcgccctc gggcacctgt
      541 cctcgctcgc cggcgaggcc cgggcgtacg tccgggcggc tctcggcgcc cgctcgcgac
      601 cgcggtaggc ctcaggcgtc cgccgccctc gggaagacgg acgaccccgc ccgccgggac
      661 tccggtgcgc ccatgaaaaa cgggcgccct cgcctttcgg cgagggcgcc cttcccggtt
      721 ccgtttccgg gctgctactt gcggatacgg aggtagaggc cgtagcggtt cgccttgaag
      781 tccgctccgg tcccgtaggt gcgggcggtc tcggcgtagt cgtcgaggtc ccactccgcg
      841 gcgatgccga agagctccgt gaagtcccag gcggcgcgca ggcgcgcccg gtcgagctcg
      901 taggcgaagc tgccgtcgtt ctcgaaggtc cggtagaggg cctcgagcgt gaagggcttg
      961 aggttcaggg tgaggccgcc ctcgagcgag tcgccccgct cgcggtagac ggagtcggcg
     1021 tcggcccagg tctggggcac ccggtaggcg atcgagtggt cgccctcgaa ccggccggct
     1081 ccgacgcgga acgtgaggaa ctcccacggg gagacctcga cgtcgccgcc gtaggtgttc
     1141 atgtcgcctt cgaagccgta gccgaagtcg tcgttggacg tgtcgacgat ctcggcgttg
     1201 ccgccgaggc ggagccactt cgcggcctgc cacgcgccgc ggaggcggag ccggtcgcgc
     1261 tcccggaagt cggtcctcac gacggcggcg tccgcctgct cgcgaatgta gtcgacgccg
     1321 agggagaggc cgccgagctt gaaggcgagg ccgccctcca tcccctggat gcagcggtcg
     1381 taggtgccgc cctggttgcc cgggacgacg acctcggcct cgtcgttctc gacggcgacg
     1441 tcggtccggt tgtcgacgaa gccgaggcgg agggtgacgg ccttgccgag cttgaggctc
     1501 gcgcggatgt cgaagatgtc ctcgttccgc tccaggcgcg tccgcgcctc gagcatcgtc
     1561 gagacgtcgc gcgggtcgaa cccgctgaac gtcgtcgtcg tcccccagag gtcgttgacg
     1621 aggctgtagc cctcgagctc gcggttgcgg cgggtgaagc cggtggagag gtcgaagccc
     1681 gccgcgaggt ggacgtcggc ccggatgctg ccgcgccagg agagcgcctc ggccgtcgcc
     1741 gccgaggact cggccaggcc cttgaaatag cggaggatct cgtacgagac gaggttgccg
     1801 gagagggtct cgtcgctcgt gtcgtcgccg tcggcatcgg cccggacgta ggtgccggtg
     1861 agccggatcg ccgagccgag cttgccgctc acgaccgccg aggtgaccgg cgtgtcgaat
     1921 tcagccttcg agcggcgcgt gagcgagtcg agcgagacgg gaacgccgag gatggtgccc
     1981 gactcgttcc cggagcccgc gaggagcttc aggttctcgt cggacttgaa cgtccgccag
     2041 ccctggacaa cttggccgga gacggggccg gcgtcgaagg cgacgccgac gcgatactcc
     2101 gtgtcgcgct ccttgaggtc ctgctggagc tggaagtcgt cctgcccgac gtggtaggtc
     2161 gtcagcccgg ggccggaata ccggctgtag ctgaagccga ggagaggacg gatcttcttg
     2221 cccgggagga gctcgaggtc gacgtcgacg gagttccggg tccggtcgta ggagtgctgg
     2281 ctgtcgaggt tgccgttccc caggaacggg ttcgcgatgg tcgggagatt cgagtagcgc
     2341 tccgagcgcc gccaggaggc gttcagcttc cagtagccgg tgcggccggt ctccacgcgg
     2401 aggaggccgt tcgggccggc gccgaggtcg gcggcgtcga tgcggaagtg gtcgacgaag
     2461 ttcgtgtcac ggatgtcgcc gaggccccac gacagggagc ggagctggag tccgtcctcc
     2521 tcgtcgagct ggctcttgaa catctcttcg ttgcccttga tgtcggtgaa gcggtagccg
     2581 atctcgacgt cgagcggcac gtcctgggcg gacgcggcgc cggcgaggag gagcgccggc
     2641 aggaggccgg cgaggaggaa tcgattcgcg cgcatctccg gctcctccta cttcagcagc
     2701 ttcggcgacc ggttggagcc gtggaccgcc acgtggcagg tcgtgcagtt ccgccagcgc
     2761 gggagggaga tgttgtgcgt cgccgggggc tgcgagccga gggcgcccgc cgcgagcgtc
     2821 gagtggcact cgaggcagag gaggtcgacg cgcgaacggg tcagctgctt gggattgttc
     2881 gagccgtggg gctcgtggca ggagagacag ccgcccgtca tgccgccgac ggtcgggccg
     2941 acgtgctcgt agacgaaggg cccgcgcttc tcggtgtggc aggtcaggca cggcgcctcg
     3001 ccctgccgcg tgagc
//
LOCUS       DAOTEZ010000491         3009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_25572_length_3009_cov_6.528097, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000491 DAOTEZ010000000
VERSION     DAOTEZ010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25572_length_3009_cov_6.528097"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="P5164_18270"
     CDS             <1..288
                     /locus_tag="P5164_18270"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRY45883.1"
                     /translation="SPWDGRQLQRLFYRVRDAAGLRKALHPHSIRHTAASWAVQSGVP
                     LLSVKETLGHANISQTVRYSHLETEHLRGAVEAVSTVEKSGRRHRGATDRG"
     gene            complement(329..1078)
                     /gene="mtgA"
                     /locus_tag="P5164_18275"
     CDS             complement(329..1078)
                     /gene="mtgA"
                     /locus_tag="P5164_18275"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HRY45884.1"
                     /translation="MTAARGASRRGRLLVLGGAALAVLLFGVVVPIPRIVALRFGPPS
                     TTAFLEARRERLLSEGRDARIDRRPVPLEKVSPHLVTAVLVSEDARFFEHHGIDWDAV
                     KAARTRNRRFPRRRPLGASTITQQLAKNLFLSPSRSWLRKGREAAVATAMELLLPKRT
                     ILEHYLSGIEWGERVWGCEAAARTYFGRPASRLTREQAAWLAAMIPGPRLFLSRPARH
                     QRRAARILARMRRAGDVRPLLLEEADDPPAR"
     gene            complement(1098..2447)
                     /gene="lat"
                     /locus_tag="P5164_18280"
     CDS             complement(1098..2447)
                     /gene="lat"
                     /locus_tag="P5164_18280"
                     /EC_number="2.6.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0045484 - L-lysine 6-transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine 6-transaminase"
                     /protein_id="HRY45885.1"
                     /translation="MSSSVIPGIGPKNVLESLGRHMLVDGYHVVMDLERSHGSFIHDS
                     RHGVEILDLFSHFATIPVGYNHPKLKDPAFLEELGRAALTKPANSDIYTEEMARFVET
                     FARIAVPPSHSEHLFFVEGGSVGVENALKAAFDWKVRKNFRKGYRREVGHQIVHFEQA
                     FHGRTGYALSLTNTADPRKTMYFPKFRWPRIVNPKLRFPVDAAEVERVEKVEALAIAQ
                     VKQALVENRDDLAALIVEPIQAEGGDNHFRTEFLAALRQLCDESEMLLICDEVQTGIG
                     LTGRMWGFQTAGVVPDLFAFGKKMQVCGFASNGRIFDEEENVFTVSSRINSTWGGSLV
                     DMVRARKYLEIIEEERLVENARVVGEVLLGELGALAADFPGRVTNVRGKGLFIAFDLP
                     DGATRGEVLSTMLQKHHVMALSSGERSIRLRPALTLGRDEALLGVKRLRAALAEVFA"
     gene            complement(2512..>3009)
                     /locus_tag="P5164_18285"
     CDS             complement(2512..>3009)
                     /locus_tag="P5164_18285"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRY45886.1"
                     /translation="GDLTLLFLDCDHLKAVNDEHGHLAGSQILREVAFILQRQLAQTT
                     SLPCRYGGDEFTVILVDCELARGMEIAERIRAAIADWTFLPMAIGPGEPALNLKGVIT
                     MSIGVASFRRHCTPGRPPSELKNEILKKADAALYAAKEAGKDCVVAARRESGPPSISN
                     VVLKT"
BASE COUNT          445 a         1007 c         1106 g          451 t
ORIGIN      
        1 tcgccctggg acggtcgcca gctacagcgg ctcttctacc gggtccgaga cgcggcgggc
       61 ctccggaagg cgcttcaccc gcattcgatc cgtcacaccg ccgcgagctg ggccgtacag
      121 tccggagtcc cgctcctctc cgtgaaggag accctcgggc acgcgaatat ctctcagacg
      181 gtccgctaca gccacctgga gacggagcac cttcgaggtg ccgtcgaggc ggtttctact
      241 gtggaaaagt cgggacggcg ccaccgcggc gccaccgatc gaggataacc cgtgacgctg
      301 ccacgctacg gctcattgcc ccgaaatctt accgcgcggg aggatcgtcg gcctcctcga
      361 gaaggagagg ccggacgtcc ccggcgcggc gcatccgggc gaggatgcgg gcggcccggc
      421 gctgatgccg ggccgggcgg gagaggaaga ggcgcgggcc ggggatcatc gcggcgagcc
      481 aggcggcctg ctcgcgcgtg agccgggacg ccgggcgccc gaagtacgtg cgggcggccg
      541 cctcgcaccc ccagacgcgc tccccccact cgatccccga caggtagtgc tcgaggatcg
      601 tccgcttcgg gaggaggagc tccatcgcgg tcgcgacggc cgcctcgcgc cccttccgga
      661 gccaggatcg cgagggggag aggaagaggt tcttcgcgag ctgctgcgtg atcgtcgagg
      721 ccccgagggg ccggcgcctc gggaagcggc ggttgcgcgt ccgggcggcc tttacggcgt
      781 cccagtcgat cccgtggtgc tcgaagaacc gggcgtcctc ggagacgaga acggcggtca
      841 cgaggtgggg ggagaccttc tcgaggggga cggggcgccg gtcgatccgg gcgtcccggc
      901 cctcggagag gagcctctcg cggcgggctt ccaggaaagc cgtcgtcgac ggggggccga
      961 agcggagggc gacgatccgg gggatcggga ccacgacgcc gaagagcaga acggcgagcg
     1021 ccgccccgcc gaggacgagg agcctcccgc ggcgcgaagc tcctcgggcg gccgtcattc
     1081 gagggcgggc ggccggctca ggcgaagact tccgcgagcg cggcgcggag gcgcttcacg
     1141 ccgaggaggg cctcgtcccg gccgagcgtc agcgcgggcc ggaggcggat cgagcgctcg
     1201 ccggaggaaa gcgccatgac gtggtgcttc tgcagcatcg tcgagaggac ctccccgcgc
     1261 gtcgcgccgt ccggcaggtc gaacgcgatg aagaggccct tcccgcggac gttcgtgacc
     1321 ctgccgggga aatcggcggc gagggcgccg agctccccga ggaggacctc cccgacgacc
     1381 cgggcgttct ccacgagcct ctcctcctcg atgatctcga ggtacttccg ggcgcggacc
     1441 atgtcgacga ggctcccgcc ccaggtggag ttgatccgcg aggagaccgt gaagacgttc
     1501 tcctcttcgt cgaagatccg gccgttcgac gcgaagccgc agacctgcat cttcttgccg
     1561 aaggcgaaga ggtcggggac gaccccggcc gtctggaacc cccacatgcg gcccgtcagg
     1621 ccgatccccg tctgcacctc gtcgcagatc agcagcatct cgctctcgtc gcagagctgc
     1681 cggagcgccg cgaggaactc ggtccggaag tggttgtcgc ccccttccgc ctggatcggc
     1741 tcgacgatga gcgccgcgag gtcgtcgcgg ttctcgacga gggcctgctt cacctgggcg
     1801 atcgcgagcg cctcgacctt ctcgacgcgc tcgacctcgg ccgcgtcgac ggggaaccga
     1861 agcttcgggt tgacgatccg cggccagcgg aacttcggga agtacatcgt cttccgcggg
     1921 tcggccgtgt tcgtcaggga gagcgcgtag ccggtccttc cgtggaaggc ctgctcgaag
     1981 tggacgatct ggtggccgac ctcgcggcgg tagcccttcc ggaagttctt ccgcaccttc
     2041 cagtcgaagg cggccttcag cgcgttctcg accccgacgg agccgccctc gacgaagaag
     2101 aggtgctcgg agtggctcgg cgggaccgcg atgcgggcga aggtctcgac gaagcgggcc
     2161 atctcctcgg tgtagatgtc gctgttcgcc ggcttcgtga gggcggcccg tccgagctcc
     2221 tcgaggaacg cggggtcctt cagcttcggg tggttgtagc cgaccggtat ggtggcgaag
     2281 tgggagaaga ggtcgaggat ctcgacgccg tgccggctgt cgtgaatgaa gctcccgtgc
     2341 gagcgctcga ggtccatcac gacgtggtag ccgtccacga gcatgtggcg gccgagcgat
     2401 tcgaggacgt tcttcgggcc gattccgggg atgacgctcg acgacattgt ccctccgccg
     2461 tgcggccccg tcccggggcc ggccccggat gttagcagcg gagcgcgccg ctcacgtctt
     2521 caggacgacg ttcgagatcg agggcgggcc gctctcgcgg cgggccgcga cgacgcagtc
     2581 tttcccggcc tccttggccg cgtagagggc cgcatcggcc ttcttcagga tctcgttctt
     2641 cagctcgctc ggaggccggc ccggcgtgca gtgccgccgg aaggacgcga cgccgatcga
     2701 catcgtgatg acgcccttca ggttcagggc aggctcgccc ggcccgatcg ccatcgggag
     2761 gaaggtccag tcggcgatcg cggcgcggat gcgctccgcg atctccatcc cgcgggcgag
     2821 ctcgcagtcg acgaggatga ccgtgaactc gtcgcctccg tagcggcagg ggagggacgt
     2881 cgtctgggcg agctgtcgct ggaggatgaa ggcgacctcc cgcaggatct ggctcccggc
     2941 gaggtgcccg tgctcgtcgt tcaccgcctt gaggtggtcg cagtcgagga agaggagcgt
     3001 caggtcgcc
//
LOCUS       DAOTEZ010000492         2990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_25798_length_2990_cov_5.382283, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000492 DAOTEZ010000000
VERSION     DAOTEZ010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25798_length_2990_cov_5.382283"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="P5164_18290"
     CDS             complement(<1..1049)
                     /locus_tag="P5164_18290"
                     /inference="COORDINATES: protein motif:HMM:NF014789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRY45887.1"
                     /translation="MSTVKTDKTVKAETEAPSFLKSLYQGRFDESLVFPYPEIPEDTK
                     ELVTAFADAYRDFDAANIDSEKIDADHFFPRDVVKGLGDLGAMGMAIPEEYGGSGFSA
                     AAYCKMMEVIGPLDASAAIVIGAHQSIGIKPLLLFGTEEQKKKWLPDLAVAKMVGAFC
                     LTEPEAGSDAASLKTTAVYDAATDEYVLTGTKQWISNGGFARFFTVFARIPEAGVEAG
                     SHKEIACFAVVANEDGTLPGLSRGVEEKKLGLCGSSTCQIILDGCRVPAFNLIGGKGN
                     GFRVAVETLNTGRTSLGAGAVGGCKQMIKLAVEHATQRKQFKTRIADFEMIREKISEM
                     TAHTYVLESMVYMTAG"
     gene            complement(1052..>2990)
                     /locus_tag="P5164_18295"
     CDS             complement(1052..>2990)
                     /locus_tag="P5164_18295"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRY45888.1"
                     /translation="ASDPAAIANGVRRGQEIFGKLANLPFPTVAVIEGACVGGGCETI
                     LAMDWRLASDSKKTQIGLPEIKLGILPAWGGTARLPRVVGLTGALDVILAGKVVDSRR
                     AKRMGLVDELVPAPILLEAARKFARGKFGTPKRANAAPDGGPLRAVPAKGMEKLLEGV
                     GKSVVFSKARSSVLRETHGNYPAPLAALAAVERGFGKPLKEALELEVEAAGKLVGSPE
                     MKALVNLFFRMEEVKKETGVAAGVSPRPVKRVGVLGAGAMGGGIAQLSADKGWPVRMK
                     DIRHEALALGYAQAAKIWQEKVKKRRMTKVEFAGKMSLLGGGLGWEGFDACDVTIEAV
                     LEKLAVKQAVLKEWEAVAAPDAIFASNTSTLPITEIAKAAARPERIVGMHFFNPVHKM
                     PLVEVIFGEKTAPEVTATIFELAKKMGKTPVVVKDAPGFLVNRILAPYLGEGVRLLLE
                     GVSMEAIDKGMRKFGMPVGPIELLDDVGIDVAAKGAETLSAAWPERMPIDPAFGRLVT
                     ATRLGRKTKKGFYRYENDKRVGPDAQAYVDLGVASPGSVSITPEALEARLIYPMVNEA
                     AFCLAEAIVPGPDKLDLAMIFGIGFPPFRGGLCAHAEARGLAAVVDALNRLASEKGPR
                     FKPAPLLADLAKQGTTFFGKE"
BASE COUNT          427 a         1080 c          994 g          489 t
ORIGIN      
        1 ccggcggtca tgtagaccat cgactcgagg acgtaggtgt gcgccgtcat ctcggagatc
       61 ttctcccgga tcatctcgaa gtcggcgatc cgggtcttga actgcttgcg ctgcgtcgcg
      121 tgctcgacgg ccagcttgat catctgcttg cagccgccga cggcgcccgc tccgagcgac
      181 gtccggcccg tgttcagcgt ctcgacggcg acccggaagc cgttcccctt cccgccgatc
      241 aggttgaagg ccggcacccg gcagccgtcc aggatgatct ggcaggtcga ggagccgcag
      301 aggccgagct tcttctcctc gacgccgcgg gagaggccgg ggagcgtccc gtcctcgttc
      361 gcgacgacgg cgaagcaggc gatctccttg tgcgagcccg cctcgacgcc cgcctcgggg
      421 atgcgcgcga agacggtgaa gaaccgcgca aaaccgccgt tcgagatcca ctgcttcgtc
      481 ccggtcagga cgtactcgtc cgtggccgcg tcgtagacgg ccgtcgtctt cagcgaggcg
      541 gcgtcggagc ccgcctccgg ctccgtgagg cagaaggcgc cgaccatctt cgcgacggcg
      601 aggtcgggga gccacttctt cttctgctcc tccgtcccga agagcagaag cggcttgatg
      661 ccgatcgact ggtgcgcccc gatgacgatg gcggccgagg cgtcgagcgg cccgatgacc
      721 tccatcatct tgcagtaggc ggctgcgctg aagccgctcc cgccgtactc ctcgggaatc
      781 gccatcccca tcgcgccgag gtccccgaga cccttcacca cgtcgcgcgg gaagaagtgg
      841 tcggcgtcga tcttctcgga gtcgatgttc gcggcatcga agtctcggta ggcgtcggcg
      901 aacgccgtga cgagctcctt cgtgtcttcc gggatctccg ggtaggggaa gacgagcgac
      961 tcgtcgaatc gcccctggta gagcgacttc aggaacgagg gggcctcggt ctcggctttc
     1021 acggtcttgt cggtcttcac ggtcgacatg gctactcctt cccgaagaac gtcgtcccct
     1081 gcttcgccag gtcggcgagg agcggggcgg gtttgaagcg ggggcccttc tcggaggcga
     1141 ggcggttcag ggcgtcgacg acggcggcga ggccgcgtgc ctccgcgtgg gcgcagaggc
     1201 cgccccggaa cggcgggaag ccgatcccaa agatcatcgc gaggtcgagc ttgtcgggac
     1261 ccggcacgat cgcctcggcg aggcagaacg ccgcctcgtt gaccataggg tagatgagcc
     1321 gcgcctcgag cgcctcgggg gtgatcgaga ccgagccggg cgaggcgacg ccgaggtcga
     1381 cgtaggcctg cgcgtccggg ccgacgcgct tgtcgttctc gtagcggtag aagcccttct
     1441 tcgtcttccg gccgaggcgc gttgcggtga cgagccgccc gaaggccggg tcgatcggca
     1501 tccgctcggg ccacgccgcc gagagcgtct cggccccctt cgccgcgacg tcgatcccga
     1561 cgtcgtcgag gagctcgatc ggcccgaccg gcatcccgaa cttccgcatc cccttgtcga
     1621 tcgcctccat cgagaccccc tcgaggagga gccggacccc ctcgccgagg tacggggcga
     1681 ggatccggtt cacgaggaag cccggcgcgt ccttcacgac gaccggcgtc ttccccatct
     1741 tcttcgcgag ctcgaagatc gtcgccgtca cctcgggcgc cgtcttctcc ccgaagatca
     1801 cctcgacgag cggcatcttg tggaccgggt tgaagaagtg catcccgacg atccgctcgg
     1861 ggcgcgcggc cgccttcgcg atctcggtga tcggcagcgt cgacgtgttc gaggcgaaga
     1921 tcgcgtcggg agcggcgacc gcctcccact ccttcaggac ggcctgcttg acggcgagct
     1981 tctcgaggac ggcctcgatc gtcacgtcgc aggcgtcgaa gccctcccag ccgagccccc
     2041 cgccgaggag cgacatcttc ccggcgaact caaccttcgt catccgacgc ttcttcacct
     2101 tctcctgcca gatcttcgcg gcctgcgcgt agccgagcgc gagcgcctcg tgccggatgt
     2161 ccttcatccg cacgggccac cccttgtcgg ccgagagctg cgcgatgccg ccccccatcg
     2221 ccccggcgcc gaggacgccg acacgcttca ccggccgcgg ggagacgccc gcggcgacgc
     2281 ccgtctcctt cttgacctcc tccatccgga agaagaggtt cacgagcgcc ttcatctcgg
     2341 gcgagccgac gagcttcccc gccgcctcga cctcgagctc cagcgcctcc ttcagcggct
     2401 tcccgaagcc cctttccacg gcggcgaggg ccgcgagcgg agccgggtag ttgccgtgcg
     2461 tctcgcggag gacggaggag cgcgccttgc tgaagacgac cgacttcccg accccctcca
     2521 ggagcttctc catccccttc gcggggacgg cgcggagcgg gccgccgtcg ggcgcggcgt
     2581 tcgcgcgctt cggcgtcccg aacttcccgc gcgcgaactt ccgtgcggct tcgaggagga
     2641 tcggcgccgg gacgagctcg tcgacgagtc ccatcctctt cgccctcctc gagtcgacga
     2701 ccttccccgc gaggatcacg tcgagcgccc cggtcaagcc gacgacgcgc ggcaggcggg
     2761 ccgtcccgcc ccaggccggg aggatcccga gcttgatctc ggggaggccg atctgggtct
     2821 tcttcgagtc ggaggcgagg cgccagtcca tcgcgaggat cgtctcgcag ccgccgccga
     2881 cgcacgcccc ttcgatcacc gccaccgtcg ggaacggcag gttcgcgagc ttcccgaaga
     2941 tctcctgccc ccggcggact ccgttcgcga tggcggccgg atccgaggcc
//
LOCUS       DAOTEZ010000493         2990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_25799_length_2990_cov_4.982283, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000493 DAOTEZ010000000
VERSION     DAOTEZ010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25799_length_2990_cov_4.982283"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1522
                     /locus_tag="P5164_18300"
     CDS             <1..1522
                     /locus_tag="P5164_18300"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRY45889.1"
                     /translation="AKVTDFGIGKLLTGDTDLTTTGQMIGSPAYMSPEQVRGDKLDVR
                     SDIFSLGVVLYQTLTLRKPFPADTLTTLVYQILHDEPLDPLRHNEELPPEISEIARKC
                     LAKNREERYGDAAELADDLRALMGIAPLASTTGLSESKVGRARAAAAAAAATAAAAPT
                     PVPGSAPQKAAPPPPVDLSASRRLSRSGGRTSMTSSQHSPAALAGAAVGGLVLIGVLV
                     GGFFFLRSQGLFRSVAVPLPEPAATPTATPTPDAEPDAPTPAPSPGASGPEASPATGA
                     VLPPPSPAPGRTAVPTRTPRPRATPTPTPLPTATPMPTPTPTPLVRPDATYITRRLVK
                     LSVSPDQARVYLDGRYVGISDDWDDAGGGSLLAFVADGRHRLRFAFPGRKDLLVDILV
                     TGNAVEERVEIERELERGSPDGPTGPDGKIPRPDYQTAGLARLAVEPPFARVTVDGHD
                     MGTAGRFVEQEMQFREPGVYDVVLTAPGYQPKRLRVVVSSVVGKDRVVLREKLKKL"
     gene            complement(1565..2494)
                     /locus_tag="P5164_18305"
     CDS             complement(1565..2494)
                     /locus_tag="P5164_18305"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRY45890.1"
                     /translation="MNETVPRDAALLAAGRAPIPLDADAPVTDGSDDVLLLTSGSVVA
                     HVLTAFGPHPIGTPAAPSLLSLHGALAGAYDLCRYDPRGGAAMVVFPGEEARSLLFAG
                     TPAGAAFRRIALSSVTTALREANATLARFFDDIVPEPGWKPKPAPAATPLPAAGPADP
                     RRVRDLFDAAGLDAAALPDLGLTQRRLPSGSRLLSAGEEAGEAYVVAEGRLRVSIRIP
                     GVGEEALAFLGPGEVVGEMALVDDAPRSADVVAQDGAATVFVLSREVFRRLLDEGTPG
                     GAPLLGGIVVALVRRFEESIRKAATFRVLSGPF"
     gene            2588..>2990
                     /locus_tag="P5164_18310"
     CDS             2588..>2990
                     /locus_tag="P5164_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRY45891.1"
                     /translation="MNAPDSPSLLRTPLFDCHKALHGKLVPFGGWEMPVQYSGLVEEH
                     AAVRTKAGLFDVSHMGEIRVRGPKAFEVVQKITCNDVAALVDGRIQYSAFLTEKGTFV
                     DDLLTYRIAEDDYLLVVNASNTPKDVAWAKSF"
BASE COUNT          425 a         1154 c         1040 g          371 t
ORIGIN      
        1 cgcgaaggtg accgacttcg ggatcgggaa gctcctgacg ggcgacaccg acctgacgac
       61 gacgggacag atgatcggct cgccggcgta catgtccccc gagcaggtgc gcggcgacaa
      121 gctcgacgtg cggagcgaca tcttctcgct cggcgtcgtc ctctaccaga ccctgaccct
      181 ccgcaagccc ttcccggccg acacgctgac gacgctcgtc taccagatcc tgcacgacga
      241 gccgctcgac ccccttcggc acaacgagga gctgccgccc gagatctccg agatcgcccg
      301 aaagtgcctc gcgaagaacc gcgaggaacg ctacggcgac gccgcggagc tggccgacga
      361 cctccgggcg ctgatgggga tcgccccgct cgcctccacg acggggctct ccgaaagcaa
      421 ggtggggcgg gcgcgggcgg cggccgccgc ggcggccgcc acggccgctg cggcgccgac
      481 gcccgtcccg ggcagcgcgc ctcagaaggc cgcgcctcct cctcccgtcg acctttccgc
      541 ctcgcgccgc ctgtcgcgat cggggggacg gacctcgatg acgtcctccc agcactcgcc
      601 ggcggcgctc gccggcgccg ccgtgggagg gctcgtcctg atcggggtcc tcgtcggcgg
      661 cttcttcttc ctgcgctcgc aggggctctt tcgctccgtc gccgtgccgc tgccggagcc
      721 ggcggccact ccgacggcga cgccgacgcc ggacgccgag ccggacgcac cgactcccgc
      781 gccctctccc ggcgcctccg gcccggaggc atcgccggcg accggcgccg tcctcccgcc
      841 gccgtcgccg gcgccggggc gaaccgccgt cccgacccga acgccccgcc cgcgagcgac
      901 gccgacgccg acgccgctcc cgacggcgac cccgatgccc accccgaccc cgactccgct
      961 cgtccgaccc gacgcgacct acatcacgcg ccggctcgtg aagctgagcg tctcgcccga
     1021 ccaggcccgc gtctacctcg acggccgcta cgtcggcatc tccgacgact gggacgacgc
     1081 cggcggggga tcgctcctcg ccttcgtcgc cgacggccgg caccggctcc gcttcgcctt
     1141 ccccggccgg aaggacctcc tcgtggacat cctcgtcacc ggcaacgccg tggaggagcg
     1201 ggtcgagatc gagcgcgagc tcgagagagg cagcccggac gggccgaccg gccccgacgg
     1261 gaagatcccg cgtcccgact accagacggc gggcctcgca cgcctcgcgg tcgagccgcc
     1321 gttcgcccgc gtcaccgtcg acggacacga catggggacg gccggccgct tcgtcgagca
     1381 ggagatgcag ttccgcgagc ccggggtcta cgacgtcgtc ctgacggctc cgggctacca
     1441 gccgaagagg cttcgggtcg tcgtttcctc tgtcgtgggg aaagaccgag tcgttctccg
     1501 ggagaagctc aagaagctct gaggagtcgg ccccgcggtg ccgagcgcgc cgacacggga
     1561 ggcgtcagaa gggcccggac aggacgcgga aggtggcggc ctttcggatc gactcctcga
     1621 accggcgaac gagcgcgacg acgatccccc cgagcagcgg cgcccctccc ggggtcccct
     1681 cgtcgagcag gcgccggaag acctccctcg agaggacgaa gacggtcgcc gccccgtcct
     1741 gcgccacgac gtctgccgag cgcggcgcgt cgtccacgag agccatctcg ccgaccacct
     1801 ctccgggccc gaggaacgcg agcgcctcct cccccacgcc cgggatccgg atggagaccc
     1861 ggagcctccc ctccgcgacg acgtacgcct cgcccgcctc ctccccggcc gagaggaggc
     1921 ggctccccga ggggaggcgc cgctgggtca ggccgaggtc ggggagagcc gcggcgtcga
     1981 ggcccgcggc atcgaagagg tcccggaccc tccgcgggtc ggccgggccg gccgcgggaa
     2041 gcggcgtcgc cgcgggcgcc ggcttcggct tccagcccgg ctccggcacg atgtcgtcga
     2101 agaatcgtgc gagggtcgcg ttcgcctcgc ggagcgcggt cgtcacgctc gaaagggcga
     2161 tccgccggaa ggcggcgccg gcaggcgtgc cggcgaagag caggctccga gcctcctccc
     2221 ccgggaagac gaccatcgcc gccccgccgc gcgggtcgta gcggcagagg tcgtacgctc
     2281 cggcgagggc gccatgcagg gagaggagcg acggggcggc gggcgtcccg atcgggtgcg
     2341 gtccgaacgc ggtcagcacg tgcgcgacga ccgagcccga agtcaggaga aggacgtcgt
     2401 cggagccgtc cgtgacgggg gcatccgcgt cgagggggat cggcgcgcgc ccggccgcga
     2461 ggagggcggc gtcccgcggg accgtttcgt tcatggctgg ccgggagtct agcgggaacg
     2521 gtcgacaccg gcctcccccc tcggttgacg ccctccactc cgggccgtat cgtgcccggc
     2581 cgggcctatg aacgctcccg attccccctc cctcctccgc acgccgctgt tcgactgtca
     2641 caaggcgctc cacggaaagc tcgtcccctt cgggggatgg gagatgcccg tccagtactc
     2701 gggcctcgtc gaggagcacg ccgccgtccg gacgaaagcc ggcctcttcg acgtctcgca
     2761 catgggcgag atccgtgtcc gcgggccgaa ggcgttcgag gtggtgcaga agatcacctg
     2821 caacgacgtc gccgccctcg tcgacggccg gatccagtac tcggccttcc tgacggagaa
     2881 ggggacgttc gtcgacgacc tcctgaccta ccggatcgcc gaggacgact acctcctcgt
     2941 cgtgaacgcc tcgaacaccc cgaaggacgt cgcctgggcg aagtccttcg
//
LOCUS       DAOTEZ010000494         2980 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_25937_length_2980_cov_4.454359, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000494 DAOTEZ010000000
VERSION     DAOTEZ010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25937_length_2980_cov_4.454359"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="P5164_18315"
     CDS             complement(<1..621)
                     /locus_tag="P5164_18315"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF21 domain-containing protein"
                     /protein_id="HRY45892.1"
                     /translation="MNDVLLLCLFALCIVLSSFFSGSETALTTLSEAAVYRLREKRHP
                     QAARLERLRERLPQTISTLLIGNNLVNIAAGSIGTVLAIDALGARWGVVAATTVTTLL
                     LLVFGEVTPKTLAARNAERVACVAAPVIDLLMVVFAPLARLLSAVAAVLLGPARGGVE
                     IDLLPEQVLGRRVRRVREPGRGAGRGFAGIGPQRRRCIKGAWAEQKV"
     gene            complement(618..1049)
                     /locus_tag="P5164_18320"
     CDS             complement(618..1049)
                     /locus_tag="P5164_18320"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HRY45893.1"
                     /translation="MSGTLLVVAGAAFRAGRLLLTRRPAGGAHAGLWELPGGKVEPGE
                     TPEDALAREWREELGVDVDRVEPWAFSSGAPHGRHVTLLVFRVPSMRGEPEPIGVAEV
                     RWTRPDDALALPLLPADRSLVERLVRGEEGLLPASPDGVGG"
     gene            complement(1046..2017)
                     /gene="queG"
                     /locus_tag="P5164_18325"
     CDS             complement(1046..2017)
                     /gene="queG"
                     /locus_tag="P5164_18325"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HRY45894.1"
                     /translation="MPGFRSRRSGPRFPAAPPPDDPVALSRAAVRAALDRGFVAAGVV
                     AGSRAEGWDGFRAFLDRGHQGGMDWLVRDAEARRTVESILPFAKAVLCVAREVPAGGP
                     GNVARYARGEDYHQAVRRDLKAVVAAIRRLSPAGAHFRVCVDTAPVLEREWAARAGLG
                     FVGKNGMLIVPGAGSHVVLGEVLTDVALAPTAASVDPTWERCGGCSACLEACPTAAIL
                     APRVVDARLCLSYLTIEKRGPFTPEEESWLGGRLFGCDDCQDACPYNAGPRWSEPAAE
                     PSASLDPAAAATLDDEGFARSFAGSAIRRATPEGLRRNARAALEAAR"
     gene            2079..>2980
                     /locus_tag="P5164_18330"
     CDS             2079..>2980
                     /locus_tag="P5164_18330"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRY45895.1"
                     /translation="MSDTPASRLYPTAVFALVAFAVASLTVRILAPFGAAIAWAIVLA
                     VAFAAPWRFLEGRLPRRRNLAAAILTTGIGLLVLLPAGFLLGVLVNEAIGVFGRASAA
                     LAARNLSELEDLLRIPAVANALVWVESRTGLTQSELLAKAGELAAGVSGLLARFSGGV
                     VKGLFDAVVSFLVTLFVLFFLFRDGRAMARAALDLVPLEETRRVAVFASLRAMLQSIF
                     RGSLLCALIQGATGGIGWAIAGLPSPVLAGAAMAVLSLLPVGGTALVWGPGAVACWLQ
                     GRHGMAVFLAIWGLVVVSTLADNVL"
BASE COUNT          377 a         1099 c         1117 g          387 t
ORIGIN      
        1 gaccttctgc tcggcccagg cgcctttgat gcagcgccgc cgctgcgggc cgattccagc
       61 gaaaccgcga ccggcacccc gaccgggttc gcgaacccgc cgtactcggc gcccgagcac
      121 ctgctcgggc aggaggtcga tctcgacgcc gccgcgggct gggccgagaa ggacggcggc
      181 gacggccgaa aggagccggg cgagcggcgc gaagaccacc atcaggagat cgatgaccgg
      241 cgctgcgacg caggcgacgc gctcggcgtt ccgcgccgcg agcgtcttcg gcgtcacctc
      301 tccgaagacg aggaggagga gcgtcgtgac ggtcgtcgcg gccacgacgc cccaccgcgc
      361 gccgagggcg tcgatcgcga ggaccgtacc gatcgagccg gccgcgatgt tcacgaggtt
      421 gttcccgatc aggagggtcg agatcgtctg cgggagccgc tcgcggagcc gctcgaggcg
      481 agccgcctgc gggtgccgct tctccctcag ccggtagacg gcggcttccg acagggtcgt
      541 cagcgcggtc tccgagcccg agaagaagct ggagaggacg atgcagagcg cgaacaggca
      601 gaggaggagg acgtcgttca tccgccgact ccgtccggcg aggccggcag gagaccttcc
      661 tcgcccctca cgagccgctc gacgagggaa cggtcggccg gaaggagcgg gagggcgagc
      721 gcatcgtccg gacgggtcca gcgcacctcg gcgacgccga tcggttcggg ctcgccccgc
      781 atcgacggga cgcggaagac gaggagcgtc acgtggcggc cgtgcggcgc gcccgaggag
      841 aaggcccagg gctcgacgcg gtcgacgtcg acgccgagct cctccctcca ctcgcgggcg
      901 agcgcgtcct cgggagtctc gcccggctcc acctttccgc ccggcagctc ccagaggccg
      961 gcgtgagcgc cgccggcggg tcgccgcgtc aggaggaggc ggcccgcgcg gaaggcggct
     1021 ccggcgacga cgaggagggt cccgctcacc gggccgcctc gagcgccgcg cgggcgttcc
     1081 ggcggagccc ctccggcgtc gcgcggcgga tcgcggagcc ggcgaaggag cgcgcgaagc
     1141 cctcgtcgtc gagagtcgcc gcggcggccg ggtcgagcga ggcggaaggc tcggcggccg
     1201 gctcgctcca gcgcggcccg gcgttgtagg ggcaggcatc ctggcagtcg tcgcagccga
     1261 agagccggcc gccgagccac gactcctcct ccggcgtgaa cggccccctc ttctcgatcg
     1321 tgaggtacga gaggcagagg cgggcgtcga cgacgcgggg ggcgaggatc gcggccgtcg
     1381 ggcacgcctc gaggcaggcg gagcagccgc cgcaacgctc ccaggtcggg tcgacggacg
     1441 cggcggtcgg cgcgagcgcc acgtcggtca ggacctcgcc gaggacgacg tgcgatcccg
     1501 ccccggggac gatgagcatc ccgttcttcc cgacgaaccc gaggccggct cgcgccgccc
     1561 actccctctc gaggacgggg gccgtgtcga cgcagacgcg gaagtgcgcg ccggcagggg
     1621 agaggcgccg gatcgcggcg acgacggctt tgaggtcgcg acggaccgcc tggtgatagt
     1681 cctcgccgcg cgcgtagcgg gcgacgttcc cggggccgcc ggcggggacc tcccgggcca
     1741 cgcagaggac cgccttcgcg aaaggaagga tcgactcgac ggtccggcgc gcctcggcgt
     1801 cgcggacgag ccaatccatc ccgccctggt gcccccgatc gaggaaggcc cggaaaccgt
     1861 cccagccctc ggcccggctc cccgcgacca cgcccgcggc gacgaagccg cggtcgaggg
     1921 ccgcgcgaac tgccgcgcgg gagagggcga cggggtcgtc gggcggaggt gcagccggga
     1981 accggggccc cgaccggcgc gagcggaaac cgggcatcgg cggcgagtct agcaacgggc
     2041 cgctatgatc tccggcgatt cccggccgag gagacgtcat gtccgacacc cccgcgtcgc
     2101 gcctctaccc gacggcggtc ttcgcgctcg tcgcgttcgc cgtcgcgtcc ctgaccgtcc
     2161 ggatcctggc cccgttcggc gccgccatcg cgtgggcgat cgtcctcgcg gtcgcgttcg
     2221 ccgctccctg gcggttcctc gagggtcggc tgccgcgccg ccggaacctc gccgcggcga
     2281 tcctgacgac ggggatcggc ctcctcgtcc tcctgccggc cggcttcctc ctcggcgtcc
     2341 tggtgaacga ggcgatcggc gtgttcggcc gggcctcggc cgcgctcgcg gcccggaacc
     2401 tctcggagct cgaggacctc ctccggatcc ccgcggtcgc gaacgcgctc gtctgggtcg
     2461 aatcccggac gggcctgacg cagtccgagc tgctggcgaa ggcgggagag ctggcggccg
     2521 gcgtctcagg cctcctcgcg cgcttctccg gcggcgtggt gaaggggctc ttcgacgccg
     2581 tcgtctcgtt cctcgtcacg ctcttcgtcc tcttcttcct cttccgcgac ggacgcgcga
     2641 tggcccgcgc ggccctcgac ctcgtcccgc tggaggagac ccgccgggtc gccgtcttcg
     2701 cgtcccttcg cgcgatgctc cagtcgatct tccggggctc gctcctctgt gcgctcatcc
     2761 agggcgcgac gggaggcatc ggctgggcga tcgcaggcct cccctctccg gtcctcgccg
     2821 gtgcggcgat ggcggtcctc tcgctcctcc cggtcggcgg cacggcgctc gtctggggac
     2881 cgggggccgt cgcctgctgg ctccaggggc gccacggaat ggcggtcttc ctcgcgatct
     2941 ggggcctcgt cgtcgtctcg acgctggccg acaacgtcct
//
LOCUS       DAOTEZ010000495         2976 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_25985_length_2976_cov_7.855871, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000495 DAOTEZ010000000
VERSION     DAOTEZ010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25985_length_2976_cov_7.855871"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="P5164_18335"
     CDS             complement(<1..326)
                     /locus_tag="P5164_18335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRY45896.1"
                     /translation="MRILQINASARSEGASSTRVANAVVERLRAANPDASLKVRDLAK
                     EPVPVLDAAELGALFTPAERRSPEQAASVAARDAVIAEVQQADAIVLGVPMYNLGNPV
                     QLKAWI"
     gene            423..1352
                     /locus_tag="P5164_18340"
     CDS             423..1352
                     /locus_tag="P5164_18340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRY45897.1"
                     /translation="MKQLDPNDLLIFARVADGGSFSRAADRLGLPKSTVSRRISLLEE
                     TLGERLMVRTTRRLRLTELGERVLEHARQVATEVEDVAALAEHRKEAPVGRLRVAMPS
                     DLAILFLADALAAFTAMHPAVSLELDLSARRVDLLGENVDVAVRIGTPPDDAALATRR
                     VGVFPIGLYASPTYLAERGDPASPDDLARHDALRLLGRDGEPARWRLLQGERSWEGVP
                     PGRVAANSPELLIRLARAGAGIASVPDLLAAPSVKRGELRRVLPAWCLPPHTVWVLFP
                     GRRLMPPKVRAFVDMLAAALPGAIPPADPAADP"
     gene            1373..>2976
                     /locus_tag="P5164_18345"
     CDS             1373..>2976
                     /locus_tag="P5164_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRY45898.1"
                     /translation="MDHAQRVLFVDPSTGFYRLRRYRVGDYFGPVDLGIHLSDRWRSL
                     NFGAGLFAGSILPGSNRLVVTGFSPAWRGFYVSSLGGAALVFDNLGLNMLSLVGKAPT
                     PSILYLNRRHGEEVDVEVVPVELGKAFARGRGGFYGLMEEVLERFGSRYETDPRVLAV
                     GPAAASTDFGAIGSAPVVKGKLTHADTWAGRGGMGSKMLQEHGVAAVIYGGTFVDEDF
                     RDRTVADSWFRERYEKKLATKDFEATTKYRFDPKFETGGTFGVNFATVKGRLMAFNYR
                     SQALPESERTALWERLVVPHFLAQFNEETIAKRQQSTCGEPCAAVCKKLRDEYKKDFE
                     PYQTMGPLCGVFDQRAAELLNGAADAAGFDAISVGGVLAWLMECLLAGELTPEELGVT
                     RLPRWEAATFDAVADSMHNAELGVELISSILSGRVDLTEGPRKWGRRVAREKGKRVLD
                     RFVYLASGRRGWMVPNQYWTPGALAPMPMMGKYYMFYGPEFFPPRTLGKVCAERMLAE
                     LAIDDLGVCRFHRGWAEEMLPEIVGSV"
BASE COUNT          406 a         1110 c         1004 g          456 t
ORIGIN      
        1 tcgatccacg ccttcagctg caccgggttc ccgaggttgt acatcgggac cccgaggacg
       61 atcgcgtcgg cctgctggac ctcggcgatg accgcgtccc tcgccgcgac cgacgcggcc
      121 tgctccggcg accgccgctc ggccggggtg aagagggcgc ccagctccgc ggcgtcgagg
      181 accgggaccg gctccttcgc caggtccctc accttcagcg aggcatcggg gtttgccgcg
      241 cgcaggcgct cgacgacggc gttcgcgaca cgggtggagc tcgccccctc gctccgggcg
      301 ctcgcgttaa tctggagaat tctcatcttc gtctccttgc ggccctgtct cagtgccaca
      361 gagaagatag ggcccaattc tgcggaacga tagacgtcga atcggatatc attattccgt
      421 aaatgaaaca attagacccg aacgacctcc tgatcttcgc ccgggttgcc gacgggggga
      481 gcttcagccg cgccgccgat cggctcggac tgccgaagtc gacggtctcg aggcggattt
      541 cgctcctcga ggagacgctc ggcgaacggc tgatggtccg gacgacgcgc cgcctccgcc
      601 tcaccgagct cggcgagcgg gtcctcgagc acgcgcggca ggtggccacc gaggtcgagg
      661 acgtcgccgc cctcgccgag caccgaaagg aggcgcccgt cggccggctg cgcgtcgcca
      721 tgccgagcga cctcgcgatc ctcttcctcg ccgacgcgct tgccgcgttc acggcgatgc
      781 atccggcggt ctcgctggag ctggacctct ccgcgcgccg cgtcgacctc ctcggcgaga
      841 acgtcgacgt cgccgtccgg atcggcacgc cgcccgacga cgcggctctg gccacgcgcc
      901 gcgtcggcgt ctttccgatc ggcctctacg cttcccccac gtatctcgcg gagcgaggag
      961 accccgcctc gcccgacgac ctcgcgcggc acgacgccct ccgtctcctc gggcgcgacg
     1021 gcgagccggc ccgctggagg ctcctccagg gagagcggag ctgggaaggg gtgccgccgg
     1081 gccgggtcgc cgcgaactcg cccgagctcc tgatccggct cgcgcgcgcc ggagccggta
     1141 tcgcgtccgt ccccgacctc ctcgcggcgc cctccgtgaa gcgcggcgaa ctgcgccgcg
     1201 tcctccccgc ctggtgcctg ccgccgcaca ccgtgtgggt cctcttcccg ggccggcggc
     1261 tcatgccgcc gaaggtccgc gcgttcgtcg acatgctcgc ggctgctctt ccgggcgcca
     1321 tccctcccgc agaccccgcc gcggacccct agaatcgagg acgaggctcc cgatggacca
     1381 cgcccagcgc gtcctcttcg ttgacccgtc gacagggttc taccggctgc ggcgctaccg
     1441 cgtcggcgac tacttcgggc cggtcgacct cggcatccac ctctcggacc ggtggcggag
     1501 cctcaacttc ggcgccggcc tcttcgccgg ctcgatcctc cccggctcga acaggctcgt
     1561 cgtcaccggc ttctcgcccg cctggcgcgg cttctacgtc tcgagcctcg gcggcgccgc
     1621 gctcgtcttc gacaacctcg gcctcaacat gctgagcctc gtcgggaagg cgccgacgcc
     1681 gtcgatcctc tatctgaacc gccgccacgg cgaggaggtc gacgtcgagg tcgtccccgt
     1741 cgaactcggg aaggccttcg cgcgcgggcg cggcggcttc tacggcctga tggaggaggt
     1801 cctcgagcgg ttcggctccc gctacgagac cgacccgcgc gtcctcgccg tcggccccgc
     1861 ggcggcgtcg accgacttcg gcgcgatcgg cagcgccccc gtcgtcaagg ggaagctcac
     1921 gcacgccgac acctgggccg ggcgcggcgg gatgggctcg aaaatgctcc aggagcacgg
     1981 cgtcgccgcg gtcatctacg gcggcacgtt cgtcgacgag gacttccgcg accggaccgt
     2041 cgccgactcc tggttccggg agcgctacga gaagaagctc gcgacgaagg acttcgaggc
     2101 gacgacgaag taccgcttcg acccgaagtt cgagacgggc gggacgttcg gcgtcaactt
     2161 cgcgacggtg aaggggcgcc tgatggcgtt caactaccgc tcgcaggccc ttccggagag
     2221 cgagcgaacg gccctctggg agcggctcgt cgtcccgcac ttcctcgcgc agttcaacga
     2281 ggagacgatc gcgaagcgcc agcagtcgac ctgcggcgag ccgtgcgccg ccgtctgcaa
     2341 gaagctgcgc gacgagtaca agaaggactt cgagccgtac cagacgatgg ggccgctctg
     2401 cggcgtcttc gaccagcgcg ccgccgagct cctcaacggc gcggccgacg ccgcggggtt
     2461 cgacgcgatc tccgtcggcg gcgtcctcgc ctggctcatg gagtgcctcc tcgccgggga
     2521 gctgactccg gaggagctcg gcgtcacgcg gctcccccgc tgggaggccg cgacgttcga
     2581 cgccgtcgcc gactcgatgc acaacgccga gctcggcgtc gagctgatct cttcgatcct
     2641 ctcggggcgc gtcgacctca ccgaagggcc gcggaagtgg gggcggcgcg tcgcgcgcga
     2701 gaaggggaag cgggtcctcg accggttcgt ctacctcgcc tccggccgcc gcggctggat
     2761 ggtgccgaac cagtactgga cccccggcgc cctcgccccg atgccgatga tgggcaagta
     2821 ctacatgttc tacggccccg agttcttccc gccgcggacg ctcgggaagg tctgcgccga
     2881 gcggatgctg gccgagctcg cgatcgacga cctcggcgtc tgccgcttcc accgcggctg
     2941 ggccgaggag atgctcccgg agatcgtcgg ctcggt
//
LOCUS       DAOTEZ010000496         2968 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_26090_length_2968_cov_7.054240, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000496 DAOTEZ010000000
VERSION     DAOTEZ010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26090_length_2968_cov_7.054240"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /gene="phoU"
                     /locus_tag="P5164_18350"
     CDS             complement(<1..632)
                     /gene="phoU"
                     /locus_tag="P5164_18350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRY45899.1"
                     /translation="MKEHFSELLERLRRQLIGMGAEVESQIRVAVEALVEGDAAKARS
                     VCERDPVVNDLEVRNEEAVIQLLATQQPVASDLRLLVAAVRINADLERIGDHAVNIAQ
                     AAERLAGVRPFKPWIDIPHMAEVVRGMLRDALDAFVRRDTVLAKKVLERDDVVDAKNL
                     SLIRELLTYMAENPALISRSIEIMLISRNLERVADLATNVAEETIFIAEGL"
     gene            complement(629..2008)
                     /locus_tag="P5164_18355"
     CDS             complement(629..2008)
                     /locus_tag="P5164_18355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY45900.1"
                     /translation="MKPSVPVRLRPAAEALALGAITVAVVGFEPRALLAGALVAAAFF
                     AIRLRRMRELDRVRQGVAVAATGTRPEEWRGLSPETAEVLEALHRFADLVSREREGSE
                     RARVLARTVFEEVPVGLVVVDRGLAVLDVNPAARVLFGVPPTATCAALHDLVRAPEVH
                     ALFRSLDGRAEDGAAPVVVRIEGREGGERFLEVAARAVPGRPVAGEPAAIGVARDVTE
                     RERTERMRRQFVADVSHELRTPLATIRAAAETLSGEDGLGEDATRLVGIVERQASQMQ
                     DLVSDLTDLSQIEAGSVTIQLEAVPVAGLLASVAKDLEAAARAREVEVSIEAPQDLAV
                     HGDRRRLAQVFRNLLDNAVKFSPAGARVELRAEAATEAGAQLARIHVVDRGIGIPASE
                     QVKIFQRFYRIDPSRAKTVPGTGLGLSIVKHLLILHGGTIRVESQPLCGSRFTVSLPA
                     AALALKEAS"
     gene            complement(2005..2709)
                     /locus_tag="P5164_18360"
     CDS             complement(2005..2709)
                     /locus_tag="P5164_18360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY45901.1"
                     /translation="MKEQGGAPARWRIAVVEDDEDLAFTLVYSLRKDGRFEPVRFAGG
                     LVALDEMTERPPDLVLLDLNLPDVDGLTICRELRKHDATRRVPILMLTARAEERDKLL
                     GFDLGADDYVTKPFSMKELLARIAARLRRGDGGASSPDELFDDGKVRVDRGRHLVERG
                     GKSVHLTKKEFDLLWLLIRSGGRVVSRETILSRLWDYAADVETRTVDVHVRSIRKKLG
                     DDVVETVVGVGYRYPG"
     gene            complement(2861..>2968)
                     /locus_tag="P5164_18365"
     CDS             complement(2861..>2968)
                     /locus_tag="P5164_18365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008685487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HRY45902.1"
                     /translation="GFFMLGKLVEFDRTDVIFTRPKEKMTEDYITGRFG"
BASE COUNT          402 a         1104 c         1050 g          412 t
ORIGIN      
        1 agcccctcgg cgatgaagat cgtctcctcc gcgacgttcg tcgcgaggtc ggccacccgc
       61 tcgaggttcc gcgagatcag catgatctcg atcgaccggc tgatgagggc ggggttctcg
      121 gccatgtagg tcagcagctc gcggatgagc gagaggttct tcgcgtcgac gacgtcgtcg
      181 cgctcgagga ccttcttggc gaggacggtg tcgcgccgca cgaacgcgtc gagggcgtcg
      241 cggagcatcc cgcggacgac ctcggccatg tgcgggatgt cgatccacgg cttgaacggc
      301 cggacgccgg cgagccgctc ggcggcctgc gcgatgttca cggcgtggtc gccgatccgc
      361 tccaggtcgg cgttgatccg cacagccgcg acgaggagcc gcaggtcgct cgcgaccggc
      421 tgctgcgtgg cgaggagctg gatgaccgcc tcctcgttcc gcacctcgag gtcgttgacc
      481 accgggtcgc gctcgcagac ggagcgcgcc ttggcggcgt cgccctccac gagcgcctcc
      541 acggcgacgc ggatctggct ctccacctcc gcgcccatcc cgatcagctg gcggcggagc
      601 cgctcgagga gctccgagaa atgttccttc atgaggcctc cttgagggcg agggcggcgg
      661 ccggcaggga gacggtgaag cggctcccgc agagcggctg ggactccacg cggatcgtgc
      721 cgccgtggag gatgaggagg tgcttgacga tggagaggcc gagcccggtc cccggcacgg
      781 tcttcgcccg cgacgggtcg atccggtaga agcgctggaa gatcttcacc tgctcgctcg
      841 cggggatccc gatgccgcgg tcgacgacgt gtatccgggc gagctgcgcg cccgcctccg
      901 tggcggcttc cgcccgcagc tcgacccgcg cccccgccgg cgagaacttg acggcgttgt
      961 ccagaaggtt ccggaagacc tgcgcgaggc gccgccggtc gccgtggacc gcgaggtcct
     1021 gcggcgcctc gatcgagacc tcgacctccc gcgcccgggc ggccgcctcg aggtccttcg
     1081 ccacgctcgc gaggaggccc gcgaccggga cggcctcgag ctggatcgtc accgagcccg
     1141 cctcgatctg ggagaggtcg gtcaggtcgg agacgaggtc ctgcatctgc gaggcctgcc
     1201 gctccacgat cccgacgagg cgcgtcgcgt cctctccgag cccgtcctcg ccggagaggg
     1261 tctcggccgc cgcgcggatc gtcgcgagcg gcgtccggag ctcgtgcgag acgtcggcga
     1321 cgaactgccg cctcatccgc tccgtccgct cccgctccgt cacgtcgcgg gcgacgccga
     1381 tcgcggccgg ctccccggcg accggccggc ccggcaccgc gcgcgcggcc acctcgagga
     1441 agcgctcgcc cccctccctg ccctcgatcc ggacgacgac cggcgcagcg ccgtcttccg
     1501 cccgcccgtc cagagaccgg aagagggcgt gcacctccgg ggcccgcacg aggtcgtgga
     1561 gggcggcaca cgtggccgtg gggggcacgc cgaagaggac gcgcgccgcc gggttcacgt
     1621 cgaggacggc gagcccccgg tcgacgacga cgaggccgac ggggacctcc tcgaagaccg
     1681 tccgggcgag gacgcgcgcc cgctcgctcc cctccctctc gcgcgagacg aggtcggcga
     1741 agcggtggag cgcctcgagg acctcggccg tctcgggcga gaggccccgc cactcctccg
     1801 gacgcgtccc cgtggccgcc acggccaccc cctggcggac ccggtcgagc tcccgcatcc
     1861 ggcgaagccg gatcgcgaag aacgccgccg cgacgagagc gcccgcgagg agggcgcgcg
     1921 gctcgaagcc gacgaccgcc accgtgatcg ctccgagcgc gagggcctcg gcggccgggc
     1981 ggagccggac cgggacggaa ggcttcatcc cgggtacctg tagccgacgc cgacgaccgt
     2041 ctccaccacg tcgtccccga gcttcttccg gatcgagcgg acgtggacgt cgaccgtccg
     2101 cgtctcgacg tcggcagcat agtcccagag ccgcgagagg atcgtctccc ggctgacgac
     2161 ccgccccccg ctccggatca ggagccagag gaggtcgaat tctttcttcg tcaggtggac
     2221 ggacttcccg ccgcgctcga cgaggtgacg cccccggtcg acccggacct tcccgtcgtc
     2281 gaagagctcg tccggcgacg aggcgccgcc gtcgccgcgc cggagccgcg ccgcgatccg
     2341 ggcgaggagc tccttcatcg agaacggttt cgtgacgtag tcgtccgcgc cgaggtcgaa
     2401 gccgaggagc ttgtcgcgct cctcggcgcg ggccgtcagc atgaggatcg gcacgcggcg
     2461 cgtcgcgtcg tgcttccgga gctcccggca gatcgtcagg ccgtcgacgt cgggcaggtt
     2521 caggtcgagg aggacgaggt ccggcgggcg ctccgtcatc tcgtcgagcg cgacgagccc
     2581 ccccgcgaac cggaccggct cgaagcgtcc gtccttccgc aggctgtaga cgagcgtgaa
     2641 ggcgagatcc tcgtcgtcct cgacgacggc gatccgccag cgcgccggcg cgcctccttg
     2701 ctccttcaac cgaacctccc cgttatgtag tcgctcgccg ggggggcttc ctcgccgggg
     2761 ggctgagctt cgcgcgcccc ccggaccccc gccctcgctc gaaggccgct gctgaggcaa
     2821 cggtgcctgc gcgcgccccc ccggaccccc caccctctca tcagccgaat ctcccggtga
     2881 tgtagtcctc ggtcatcttc tccttcgggc gggtgaagat gacgtcggtc cggtcgaact
     2941 cgacgagctt gccgagcatg aagaagcc
//
LOCUS       DAOTEZ010000497         2967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_26105_length_2967_cov_3.268544, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000497 DAOTEZ010000000
VERSION     DAOTEZ010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26105_length_2967_cov_3.268544"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..503)
                     /locus_tag="P5164_18370"
     CDS             complement(120..503)
                     /locus_tag="P5164_18370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45903.1"
                     /translation="MRKPGTKRTPAEAPARKTTATLDLDSHQTRMVAFLAERLGGTTD
                     DVLEFLVTAGLYVAQLDPMAEGRVADGLDRIFAGTDIATEVMNELRDIECNRQWQVEA
                     FVASFTRPRVVGDWRGEPRPVQAEA"
     gene            complement(652..849)
                     /locus_tag="P5164_18375"
     CDS             complement(652..849)
                     /locus_tag="P5164_18375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45904.1"
                     /translation="MMPRYGGRKPLTEAGPGLHVAARLPRELVDDLKAMAEEIGEPVS
                     EIVRRGAASEVKKWKKRTGRA"
     gene            complement(846..1406)
                     /locus_tag="P5164_18380"
     CDS             complement(846..1406)
                     /locus_tag="P5164_18380"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRY45905.1"
                     /translation="MAGAVDPAEHVGLAVMLARKYGPGVRDPEGVLEVAVLALLGAAR
                     RFDPALGAWGAYASTSVVAAVRGEVGRQLRAPRETSLYYVTADGDEVERSDLPHVEPL
                     DGTRLLAASVRSAVAGLPEREARIVRLRYGLDGEPWALDAIGADLGLSRSRVAQLEAR
                     ALERLRRSLRYAPLDTGHKRATIGEP"
     gene            complement(1391..>2967)
                     /locus_tag="P5164_18385"
     CDS             complement(1391..>2967)
                     /locus_tag="P5164_18385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45906.1"
                     /translation="REKQMSVEQPPFVTLLKNTRNDPAGFAELALRIPEWTDTYGRPR
                     RLRRWQEDLCEEIRVRLARGERQLVVTVRSCHGAGKTFLGALLVNWFLFTRTDSRVLT
                     TAPTWAGVEGLIWPEIKRLYLGSLLPSIPLGRCLTTRLDVSDTWFAIGASSDRPENLE
                     GHHSPTAALRLVDEAKAVPVEVFDSTEGLLDAPESLDLWISTPALRSGRFYEREARGE
                     EDVVRAVVTVDDLIADGIPGKAEWKARRLKEWGEQSAEYQSRAMASYIDQAEGALFPY
                     SWIDRAMSAPFEVLGAPVAGFDVAGSKDGDESVVALAFGPDEEGRHAVTFAGRWKETD
                     TMVSKGRALALAREAGASVVRVDCIGLGKGVHDALAADVATEEYRASSRPADPTRFVN
                     RKAEDLWHVRELLEKGLLRLPAMQDLKAQLLGMRYSINAAGKVQAIDPADSPDLVDAL
                     TIALAGHGSSPHAGFLAYMDRCLEERSKGEAPAPVSPGPIGACGIPMATAVHDMSSWG
                     QAITNAARQMSGWPGR"
BASE COUNT          391 a         1089 c         1034 g          453 t
ORIGIN      
        1 cgatcgtctt cgtccggcgc ttcagagtgc ccggcacgac gattgagaac gtggccgtct
       61 tcgtcttccc ggcgtaccgg aaacccggcg tgccgctctt cttctggcct ctcagcacat
      121 cacgcctccg cctggacggg cctcggctcg ccgcgccagt cgccgacgac ccggggccgg
      181 gtgaaggaag cgacgaacgc ctcgacctgc cactgccggt tgcactcgat gtcccgcagc
      241 tcgttcatca cctcggtcgc gatgtcggtt cccgcgaaga tgcggtcgag gccgtcggcc
      301 acgcgcccct cggccatcgg gtcgagctgc gcgacgtaca ggcccgcggt gacgaggaac
      361 tcgaggacgt cgtcggtcgt cccgccgagc cgctcggcga ggaacgcgac catccgcgtc
      421 tggtgcgagt cgaggtcgag agtcgcggtc gtcttccgcg cgggcgcctc ggccggggtt
      481 ctcttcgttc cgggcttcct catggcttgc tccttctgcc cgcttcatcg gggcgggcgt
      541 cccgttcgat acatcgttcg ccgttcccct cgagccggtc cgccaggtcg tgccggccag
      601 cggctcggag tcggccgatc gcgtgtcgcc agtcgccggg cgtccacggg gtcaggcgcg
      661 gcccgttctc ttcttccact tcttcacctc gcttgcggca ccccgccgga cgatctccga
      721 gacgggctcg ccgatctcct cggccatcgc cttgaggtcg tcgaccagct cgcgcggcag
      781 gcgcgccgcc acatggaggc cggggccggc ttcggtcagg ggttttcgtc ctccgtatcg
      841 cggcatcatg gctcccctat cgttgcacgt ttgtgtcccg tgtcaagagg agcgtaccgg
      901 aggctgcggc ggagcctctc gagcgcccgg gcctcgagct gcgcgacacg agagcgggac
      961 agaccgaggt cggcgccgat cgcgtcgagc gcccacggct cgccgtcgag cccgtaccgg
     1021 agccggacga tccgggcctc gcgctcgggc aggcccgcca cggcggagcg gaccgacgcg
     1081 gccaggaggc gcgtcccgtc gagcggctcg acgtgcggca ggtcggagcg ctcgacctcg
     1141 tcgccgtcgg ccgtgacgta gtagagcgac gtctcacgcg gcgcccgcag ctgccgccct
     1201 acctcgcccc ggacggccgc cacgacgctc gtggacgcat aggcgcccca cgccccgagg
     1261 gccggatcga accgcctggc ggccccgagg agcgcgagga cggccacctc gaggacgccc
     1321 tccggatcgc ggacgccggg gccgtacttc ctcgcgagca tcacggccag cccgacgtgc
     1381 tcggcggggt ctaccgcccc ggccatcccg acatctgccg cgcggcgttc gtgatggcct
     1441 gcccccacga gctcatgtcg tgaaccgcgg tcgccatcgg gattccacag gcgccgatcg
     1501 gaccgggcga cacaggcgcg ggcgcctcgc ccttgctccg ctcctcgagg cacctgtcca
     1561 tgtaggccag gaaccccgcg tgcggggagc tgccgtggcc ggcgagcgcg atcgtcagcg
     1621 cgtcgacgag gtccggcgag tcggcggggt cgatcgcctg caccttcccc gcggcgttga
     1681 tgctgtagcg catcccgagg agctgcgcct tgagatcctg catcgcgggg aggcggagca
     1741 gccccttctc gaggagctcg cggacgtgcc acagatcctc cgccttgcgg ttcacgaacc
     1801 tcgtggggtc cgccggacgc gaggaggccc ggtactcctc ggtcgcgacg tcggccgcca
     1861 gagcgtcgtg tacgcccttc ccgaggccga tgcagtcgac ccggacgacc gacgccccgg
     1921 cctcccgggc gagggcgagc gcccgcccct tcgataccat cgtgtcggtc tccttccagc
     1981 ggccggcgaa ggtgacggcg tggcggccct cctcgtcggg gccgaacgcg agggccacga
     2041 ctgactcgtc cccgtccttc gagccggcca cgtcgaaccc cgcgaccggg gcgccgagga
     2101 cctcgaacgg ggcggacatc gcgcggtcga tccacgagta cgggaacagg gcgccttcgg
     2161 cctggtcgat gtaactcgcc atcgcgcgcg actggtactc ggccgactgc tcgccccact
     2221 ccttcagacg gcgggccttc cactccgcct tgccggggat gccgtcggcg atcaagtcgt
     2281 cgaccgtgac gacggcgcga acgacgtcct cttccccgcg ggcctcgcgc tcgtagaagc
     2341 gaccggaccg gagggccggc gtggagatcc acaggtcgag actctcgggc gcgtcgagca
     2401 gcccctcggt cgagtcgaat acctcgacgg gaaccgcctt ggcttcgtcg acgaggcgca
     2461 acgcggccgt cggggaatgg tggccctcga ggttctcggg ccggtcgctc gaggcgccga
     2521 tcgcgaacca cgtatcggac acgtccagtc gcgtcgtgag gcaccttccg agcgggatcg
     2581 acggcaggag cgacccgagg tagaggcgct tgatctcggg ccatatgagg ccctctacgc
     2641 ccgcccacgt gggcgcggtc gtgaggaccc gggagtcggt ccgggtgaac aggaaccagt
     2701 tgacgaggag cgcgccgagg aacgtcttcc ccgccccgtg gcagctgcgg accgtcacga
     2761 cgagctgccg ctcgccccgg gcgaggcgga cccggatctc ctcgcagaga tcctcctgcc
     2821 acctccggag gcgccggggc cggccgtacg tgtcggtcca ctcggggatg cgaagggcga
     2881 gctcggcgaa ccccgccggg tcgttccgcg tgttcttcag gagggtgacg aaaggaggct
     2941 gctcgacgct catctgtttc tcccggg
//
LOCUS       DAOTEZ010000498         2960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_26194_length_2960_cov_5.568675, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000498 DAOTEZ010000000
VERSION     DAOTEZ010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26194_length_2960_cov_5.568675"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1969)
                     /locus_tag="P5164_18390"
     CDS             complement(<1..1969)
                     /locus_tag="P5164_18390"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 36"
                     /protein_id="HRY45907.1"
                     /translation="MSQPPARRVDAANAYGRFTDDGLEFVVTDPRTPRPWVNVVANRR
                     AGLVVSQTGSGFTWVDNSQLAVVTRWEQDLTRDSSGRFLWLRDVETGEAWPLAPAPSL
                     PAYDRFECRHGIGSTTFRAALHGVGSEWTLFVDPEATAELWRLRLTNESGAPRRLELV
                     AFVEWNCGVTPSPRREFTKLFLECERDAERNAVLARSHMWEVGSARWGHWNTSFPYVS
                     AFACTQRVAEAEGDKAAFLGRLGSFAAPAALSGAPWPGLFGRHGDAVGALRVPVELAA
                     GASLDLGFTIATSETEENVRALLARYDAVAAFDASLAAAREGWRRLLAAHRVETPDAT
                     FDAFLNDWVRYQAISARIQGRCGYYQQSGAFGFRDQLQDSQVWLTIDPARCREQIALH
                     ATHQFADGSVYHWWHPLSELGHVTKMTDDLLWLAFVAAAYVKETGDFSILADRTPFLD
                     DPQPAPLSEHVLRAFERVFRRTSPRGLPYIGAGDWNDGLSAVGLEEKGESVWLAHFLA
                     GLLADWSELWRRTGEPDRASDFARRREALVGAINEHAWDGEWYWRATLDSGEKLGSLE
                     SARGAIYLNAQTWAVLNDVAPPGRAAAAMAAVKERLVSDAGALLLAPAYDRPVREIGY
                     ITRYAPGMRENGGVYTHAATWAIAAAAKAKDP"
     gene            complement(2010..>2960)
                     /locus_tag="P5164_18395"
     CDS             complement(2010..>2960)
                     /locus_tag="P5164_18395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="coagulation factor 5/8 type domain-containing
                     protein"
                     /protein_id="HRY45908.1"
                     /translation="PEKRVFYGLLPESISHEGYSAKPMHSYWDGFFALRGLKDAVFLA
                     TFLGKEDEAKAFAAIRDELAGDLHASIRLVMKRHGIDYVPGCAELGDFDATSTTAALS
                     PGGEEARLPRAALERTFGRYLESLAKREAGEGDSYTPYEWRNVGALVRLGRRDEALRV
                     AESLLRDLRPDEWNQWGEVVWRDPRTPKFVGDMPHTWVGSDFARSFLDLFAYEREEDS
                     SLVVGAGLPARWVRSERGVSVRGLGTPYGPLDLSLAAEGAGVRVRLSGLREVPPGGLV
                     LKPPLPAPPRTATVDGRPLPLSPAGEVVVRTLPAEVVVRP"
BASE COUNT          414 a         1060 c         1117 g          369 t
ORIGIN      
        1 cggggtcctt cgccttcgcc gcggccgcga tcgcccatgt cgccgcgtgc gtgtagacgc
       61 cgccgttctc gcgcatcccc ggcgcgtagc gggtgatgta gccgatctcc cggacggggc
      121 ggtcgtaggc cggcgcgagg aggagcgcgc ccgcatcgct cacgagccgc tccttcacgg
      181 ccgccatcgc cgccgcggcc cgccccggcg gggcgacgtc gttcaggacg gcccacgtct
      241 gcgcgttcag atagatcgcg ccgcgcgcgc tctccaggct cccgagcttc tcgccggagt
      301 cgagcgtcgc gcgccagtac cactccccgt cccaggcgtg ctcgttgatc gcgccgacga
      361 gcgcctcgcg gcgtcgtgcg aagtccgacg cccggtccgg ctcccccgtc cgccgccaga
      421 gctcgctcca gtcggcgagg agccccgcga ggaaatgggc gagccagacc gactccccct
      481 tctcctcgag tccgacggcg gagaggccgt cgttccagtc gcccgccccg atgtacggca
      541 ggccgcgcgg gctcgtccgg cggaagaccc gttcgaaggc gcggaggacg tgctcggaga
      601 gcggcgccgg ctgagggtcg tcgaggaacg gcgtccggtc cgcgaggatc gagaagtccc
      661 ccgtctcctt cacgtacgcc gccgcgacga acgcgagcca gaggaggtcg tccgtcatct
      721 tcgtgacgtg gcccagctcc gagagcgggt gccaccagtg gtagacggag ccgtcggcga
      781 actggtgcgt cgcgtggagc gcgatctgct cccggcagcg cgccgggtcg atcgtcagcc
      841 agacctgcga gtcctggagc tggtcgcgga agccgaaggc gccgctctgc tggtaatagc
      901 cgcagcgccc ctggatccgg gcggagatcg cctggtagcg cacccagtcg ttcaggaagg
      961 cgtcgaacgt cgcgtcgggc gtctcgacac ggtgcgccgc gaggaggcgg cgccacccct
     1021 cgcgtgccgc cgcgagcgag gcgtcgaacg ccgcgacggc gtcgtagcgc gcgaggagcg
     1081 cgcggacgtt ctcctccgtc tcggaggtcg cgatcgtgaa gccgaggtcg agcgaggcgc
     1141 ccgcggcgag ctccaccggc acgcggagcg cgccgacggc gtcgccgtgc cggccgaaga
     1201 ggccgggcca gggggcgccc gagagcgccg cgggcgcggc gaacgagccg agccgtccga
     1261 ggaaggcggc cttgtcccct tccgcctcgg cgacgcgctg ggtgcaggcg aacgcgctca
     1321 cgtacgggaa ggaggtgttc cagtgccccc agcgggccga cccgacctcc cacatgtgcg
     1381 accgggcgag gacggcgttc cgctccgcgt cccgctcgca ctcgaggaag agcttcgtga
     1441 actcccgccg gggcgacggc gtcaccccgc agttccactc cacgaaagcg acgagctcga
     1501 ggcggcgcgg agcgccgctc tcgttcgtca gccggagcct ccagagctcg gccgtggcct
     1561 ccgggtcgac gaagagcgtc cactccgagc cgacgccgtg gagcgcggcg cggaacgtcg
     1621 tggagccgat cccgtgccgg cactcgaagc ggtcgtaggc ggggagcgag ggcgcggggg
     1681 cgagcggcca cgcctccccc gtctcgacgt cccgcagcca gaggaagcgc ccggaggagt
     1741 cgcgcgtcag gtcctgctcc cagcgggtga cgacggcgag ctgggagttg tcgacccacg
     1801 tgaagccgga gccggtctgg ctgacgacga ggccggcgcg gcggttcgcg acgacgttca
     1861 cccaggggcg cggcgtgcgc gggtcggtga cgacgaactc gagaccgtcg tccgtgaagc
     1921 gcccgtaggc gtttgcggcg tcgacgcggc gcgcgggggg ctggctcatg agtcctccga
     1981 aagagcgttc gcgggccgcc ggggccgcgt cacggccgga cgacgacctc ggcgggaagg
     2041 gtgcggacga cgacctctcc ggccggggag agaggcaggg gccggccgtc gacggtcgcc
     2101 gtccgcgggg gcgcgggaag gggcggcttc aagaccaggc cgccgggagg gacctcgcgc
     2161 aggcccgaga ggcggacgcg gacgcccgcc ccttcggccg cgagggagag gtcgagggga
     2221 ccgtacgggg tgccgagccc cctcaccgag acgccgcgtt ccgagcggac ccaccgcgcc
     2281 gggagcccgg cgccgacgac gagagacgag tcttcctccc gctcgtacgc gaagaggtcg
     2341 aggaacgaac gggcgaagtc ggagccgacc caggtgtgcg gcatgtcgcc gacgaacttc
     2401 ggggtccgcg ggtcacgcca gacgacctcg ccccactggt tccattcgtc cgggcgcagg
     2461 tcgcggagga ggctctcggc gacgcgaagc gcctcgtccc gccggccgag ccgcacgagg
     2521 gcgccgacgt tccgccactc gtacggcgtg taggagtccc cctcccccgc ctcgcgcttc
     2581 gcgaggctct cgaggtaccg cccgaacgtt cgctcgagcg cggcgcgcgg cagacgcgcc
     2641 tcctcgccgc ccggcgagag ggctgccgtc gtcgacgtcg cgtcgaagtc gccgagctcg
     2701 gcgcagcccg gcacgtagtc gatcccgtgc ctcttcatga cgaggcggat cgaggcgtgg
     2761 aggtcgccgg cgagctcgtc gcggatcgcg gcgaacgcct tcgcttcgtc ctccttaccg
     2821 aggaacgtcg cgaggaagac ggcgtccttc aagccacgga gggcgaagaa gccgtcccag
     2881 taggagtgca tcggcttcgc cgagtacccc tcgtggctga tcgactcggg gaggaggccg
     2941 tagaagaccc gcttctccgg
//
LOCUS       DAOTEZ010000499         2953 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_26272_length_2953_cov_5.845756, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000499 DAOTEZ010000000
VERSION     DAOTEZ010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26272_length_2953_cov_5.845756"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="P5164_18400"
     CDS             <1..790
                     /locus_tag="P5164_18400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRY45909.1"
                     /translation="DLLKLAPGLDANGLLGGTLSPEDGSASPMLSSAAFHRRAVDLGV
                     ECRFNERVTAIMRRKGAVVGVRTDKGGYATSCVVNAAGAWARSLAHLGGQDVPVNPDC
                     HEAGITEPARRFLEPMVVDIRRTPGAANYYFYQAHSGQVIFCVTPDPPLLGTDRRETS
                     EFLPLIARRMVGLMPKLANLKVRRTWRGLYPMTPDGSPVVGKSESLRGYVDAVGMCGQ
                     GYMLGPGVGALVARMVTETTTGEDAAILHELRPEREFAGVEKLK"
     gene            890..1201
                     /locus_tag="P5164_18405"
     CDS             890..1201
                     /locus_tag="P5164_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015186314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRY45910.1"
                     /translation="MNASVAERFPLSESEIEAFCRRHHIRSLALFGSVLREDFGPESD
                     VDVLVEFEEGRVPGFDFVRIQDELSRLLGGRKVDLVTPRALSPFLRERVLRSAVTRYA
                     A"
     gene            1191..1526
                     /locus_tag="P5164_18410"
     CDS             1191..1526
                     /locus_tag="P5164_18410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRY45911.1"
                     /translation="MRHDDQVYLEHMLDVAVRAHAKVTGLSEEAWEADEDVRLAVTHL
                     VQTLGEAARNVSDEGRSANPEIPWREAVGMRNVIVHDYLGVDDRIVRAVALRDLPALI
                     EALRRILGA"
     gene            complement(1566..>2953)
                     /locus_tag="P5164_18415"
     CDS             complement(1566..>2953)
                     /locus_tag="P5164_18415"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45912.1"
                     /translation="STDGGASWTVALPRKAAHSVATGDGLVLVGTNGDGVLRSTDGGK
                     SWEETTTDAKWVGNIVYAIVASTATPGLCYASIPNRVLRSTDGGATWKTFSRGFNWVN
                     FRSLALDEKSGAVWAGSGRDGVAKTTDGGATWTTGAGFLALEVKAILLDPSAPKRLFV
                     GTTQGGVHLSEDGGATWALSNEGLTNRAVHSLAADPSAPGTFLAGTRKGAFRSTDGGR
                     SWTHVLKGGCEPEVQHLRYAPSDPKRVYAHSGRDFCQVARSDDGGLTWRDLKAPRDAS
                     TLLGHFAFHVDAADADRVLYSDDRHLYLSADGGATWTTGTGIPPTSRIQAIVAGSGAG
                     ELFAATNKGIYRSPDGGKTWATSGPGTEKWNVRRILLDPASGTLYAGAWKEGVRRSKD
                     GGKSWTRIGGEPPHPDVVALALESPASLLVGFEGNGVWRLDLATAASAASSPAAPKPA
                     APRARPAAGKK"
BASE COUNT          399 a         1037 c         1080 g          437 t
ORIGIN      
        1 cgacctcctg aagctcgcgc ccggcctcga cgcgaacggg ctcctcggcg ggaccctctc
       61 cccggaggac ggctcggcct cgccgatgct ctcctccgcg gcgttccacc gtcgcgccgt
      121 cgatctgggc gtcgagtgcc ggttcaacga gcgcgtcacc gcgatcatgc gaaggaaggg
      181 cgccgtcgtc ggcgtgagga ccgacaaggg gggctacgcc acctcctgcg tcgtgaacgc
      241 cgcgggggcc tgggcgcgaa gcctcgcgca cctcggcggg caggacgtgc cggtaaaccc
      301 cgactgtcac gaggcgggga tcaccgaacc ggcccggcgg ttcctcgagc cgatggtcgt
      361 cgacatccgg cggacgccgg gcgcggcgaa ctactacttc taccaggcgc actcggggca
      421 ggtgatcttc tgcgtcacgc ccgacccgcc gctcctcggg acggaccggc gcgagacgtc
      481 ggagttcctg ccgctcatcg cgcggcggat ggtcggcctg atgccgaagc tcgcgaacct
      541 gaaggtccgc cgcacgtggc gcggcctcta cccgatgacg cccgacggct cgccggtcgt
      601 cgggaagagc gagtcgctcc gcggctacgt cgacgccgtc gggatgtgcg ggcagggcta
      661 catgctcggc ccgggcgtcg gcgcgctcgt cgcgcggatg gtgacggaga cgacgacagg
      721 ggaggacgcg gcgatcctcc acgagctgcg gcccgagagg gagttcgcgg gggtcgagaa
      781 gctgaagtag ggggagccgt gcgggcgcga gcggcgggct ggggggttcg agggagcgac
      841 ccgttcgggt cgctccccgg acgcgctccc tctcgccgat aatcggcccg tgaacgcgtc
      901 cgtggccgag cggtttccgc tcagcgagag cgagatcgag gccttctgca ggaggcacca
      961 cattcggtcg ctggccctgt tcggctcggt gctccgggag gacttcgggc cggaaagcga
     1021 cgtcgacgtc ctcgtggagt tcgaggaggg gcgagtcccc ggattcgact tcgtccggat
     1081 ccaggacgag ctctcccggc tcctgggagg acgaaaggtg gacctcgtga cgccccgggc
     1141 gctcagccca ttcctgaggg agagggtcct tcgatccgcc gtcacccgct atgcggcatg
     1201 acgaccaggt ctacctcgag cacatgctcg acgtggccgt ccgggcgcac gcgaaggtca
     1261 cgggcctgtc cgaggaggcg tgggaggcag acgaggatgt ccgcctcgcg gtgacccatc
     1321 tcgttcagac cctcggcgag gccgcgcgaa acgtctcgga cgaaggacga tcggcgaatc
     1381 ccgagatacc ctggcgagag gcggtcggga tgcggaacgt gatcgtccac gactatctcg
     1441 gcgtggacga ccgaatcgtc cgcgccgtcg cgctccggga cctgcccgcg ctgatcgagg
     1501 cgctccgcag aatcctcgga gcgtgacgac tcggtccgtc ccaccccgcc cctcgacctc
     1561 gtcgtctact tcttccccgc cgccggccgc gcgcgcggtg ccgccggctt cggcgccgcg
     1621 ggcgacgagg cggccgacgc ggcagtcgcc aggtcgagac gccagacgcc gttcccttcg
     1681 aagccgacga gaaggctggc cggcgactcg agggcgaggg cgacgacgtc ggggtgcggc
     1741 ggctcgccgc cgatccgcgt ccagctcttg ccgccgtcct tcgagcggcg gactccctcc
     1801 ttccacgccc cggcgtagag ggtgccggag gccgggtcga ggaggatccg cctcacgttc
     1861 cacttctcgg tccccgggcc ggaggtcgcc cacgtcttcc ctccgtccgg ggagcggtag
     1921 atccccttgt tcgtcgccgc gaagagctcg ccggcgccgc tgccggcgac gatcgcctgg
     1981 atgcgggacg tgggcggaat tccggttccg gtcgtccagg tcgcgccgcc gtcggcggag
     2041 aggtagaggt gccggtcgtc gctgtagagg acgcggtcgg cgtccgccgc gtcgacgtgg
     2101 aacgcgaagt ggccgaggag cgtgctcgcg tctcgcggcg ccttgaggtc gcgccacgtg
     2161 aggccgccgt cgtcgctccg cgccacctgg cagaagtcgc ggccgctgtg cgcgtagacg
     2221 cgcttcgggt cggacggcgc gtagcggagg tgctgcacct cgggctcgca gccacctttc
     2281 aggacgtgcg tccagctccg gcctccgtcc gtcgaccgga acgcgccctt gcgcgtgccg
     2341 gcgaggaacg ttccgggagc ggagggatca gcggcgagcg agtggaccgc gcggttggtg
     2401 agcccctcgt tggagagcgc ccacgtcgcg ccgccgtcct cggagaggtg gacgcctccc
     2461 tgagtcgttc ctacgaagag acgtttcgga gcggacggat cgaggaggat cgccttcacc
     2521 tcgagcgcga ggaacccggc gccggtcgtc caggtcgccc cgccgtccgt cgtcttcgcg
     2581 acgccgtccc ggccgctccc ggcccagacg gcgccgctct tctcgtcgag cgcgagcgag
     2641 cggaagttca cccagttgaa gccgcgggag aacgtcttcc acgtggcgcc gccgtccgtc
     2701 gaacggagga cgcggttcgg gatgctcgcg tagcagagcc cgggcgtcgc cgtcgaggcg
     2761 acgatcgcgt agacgatgtt gccgacccac ttcgcgtcgg tcgtcgtctc ttcccagctc
     2821 ttcccgccgt cggtcgagcg gagcaccccg tcgccgttcg tccccacgag gacgagaccg
     2881 tctcccgtcg cgacggaatg cgcggccttc ctcgggaggg cgaccgtcca ggacgccccg
     2941 ccgtccgtgg aga
//
LOCUS       DAOTEZ010000500         2932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_26539_length_2932_cov_4.203684, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000500 DAOTEZ010000000
VERSION     DAOTEZ010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26539_length_2932_cov_4.203684"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="P5164_18420"
     CDS             complement(<1..722)
                     /locus_tag="P5164_18420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRY45913.1"
                     /translation="MSDKIRTGIAGCGYLGRHHARILTELPASEPVGFVEANDATAAE
                     IEAKHGASGLVRCRSVAELLERGATAVIVATPTSTHAEVAEELLAGGADVMVEKPMTV
                     TLEEADRLISIAREKGRVLQVGHIERFNPAVVAVLPLVTEPKFIEAHRLGVFVPRALD
                     VDVVLDLLVHDLDIALEIVKKPVTAIHAVGVPILSRKVDIASIRIEFEGGCVANLTAS
                     RVSTEKTRKFRFFQPDWYFSIDT"
     gene            complement(722..1360)
                     /locus_tag="P5164_18425"
     CDS             complement(722..1360)
                     /locus_tag="P5164_18425"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRY45914.1"
                     /translation="MPAPVRAVLFDMDGVLVFSEDAWFSVFNETLTGFGHRPVSRAEF
                     DAIYGNGAAADRDAYMPERRVEEIDAAYARLFEAHLGEIRPNAEAVPVLAELRRRGIR
                     TSVATNTTAPLARRVLALHGLLPLLDATASADEAGAGKPDPAVVRLAAARVGEPLEAC
                     LFVGDSRYDEAAGAAAPVRFVGLAHGTGTRIEQLGELLALVPAAAAPLNSPV"
     gene            complement(1411..2373)
                     /locus_tag="P5164_18430"
     CDS             complement(1411..2373)
                     /locus_tag="P5164_18430"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRY45915.1"
                     /translation="MRLLVVKLSSLGDVAHAVPSVAALARRFPRAEIDWLVEPPAAPF
                     VAELPWVAETVVVDVRRAFSAGWAELFAERERVAALLVARRYELVVDLQGLLRSSLWT
                     VWSGADHRVGRGRWPWLHRRVPMYDRRRTPHAIENTARAVEALGASVWEGWEEAQRGL
                     APLAARLRAAGSREAERLGLPRRFTAAFPMSSGQARSIPPEWLESWPGPPLVLLGDER
                     LADHRPGREGILNCAGAMPLLGSLGLALAAERVVSADTGPGHMAALLGAESLSILGPT
                     KPARAGLRGPRARSIGAPCGGCEGKRCRRAARCLADAFEIARTR"
     gene            2434..>2932
                     /locus_tag="P5164_18435"
     CDS             2434..>2932
                     /locus_tag="P5164_18435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRY45916.1"
                     /translation="MPRVAVVIATHRRPALLAEALSSVAAQTFRDVETLVAEDGGTEE
                     TARVVAASGAPGARHVPLAFCGRPGPVRNEALRRVSSPLVAFLDDDDLWLPRRLEAHV
                     VALEAVPAAGLVFGPVRRFGDAEGTWPRSVPPRVDLARLLRGNCVPLSSVLARREALE
                     RAGLFP"
BASE COUNT          389 a         1103 c         1072 g          368 t
ORIGIN      
        1 gtgtcgatcg agaagtacca gtcgggctgg aagaagcgga acttccgcgt cttctccgtc
       61 gagacgcgcg aggcggtcag gttcgcgacg cagcccccct cgaactcgat ccggatcgag
      121 gcgatgtcga ccttcctgga gaggatcggg acgccgaccg cgtggatcgc cgtgaccggc
      181 ttcttcacga tctcgagcgc gatgtcgagg tcgtggacga ggaggtcgag gacgacgtcg
      241 acgtcgagcg cgcgggggac gaagacgccg agccggtgcg cctcgatgaa cttcggctcc
      301 gtgacgagcg gcaggaccgc gacgacggcc gggttgaacc gctcgatgtg ccccacctgc
      361 aggacacgcc ccttctcccg cgcgatcgag atgagccggt ccgcctcctc gagcgtcacc
      421 gtcatcggct tctcgaccat cacgtcggct ccgccggcga ggagctcctc ggcgacctcc
      481 gcgtgcgtcg acgtcggggt cgcgacgatg accgcggtgg cgcccctctc gagcagctct
      541 gccacggacc ggcaccggac gaggccggac gcgccgtgct tcgcctcgat ctcggcggcc
      601 gtggcgtcgt tcgcctcgac gaaaccgacg ggctcggagg cgggaagctc ggtgaggatt
      661 cgggcgtggt ggcgacccag gtagccgcag ccggcgatgc cggtcctgat cttgtcggac
      721 atcaaaccgg ggagtttagc ggagccgccg cggccggcac gagcgcgagg agctccccga
      781 gctgctcgat ccgtgtcccg gtgccgtgcg cgagcccgac gaagcggacg ggggccgccg
      841 cgccggccgc ctcgtcgtag cgggagtcgc ccacgaacag gcacgcctcg agcggctcgc
      901 cgacgcgcgc cgcggcgagg cgcacgacgg ccgggtcggg ctttcccgcc cccgcttcgt
      961 ccgccgaggc cgtcgcgtcg aggagcggaa ggaggccgtg gagcgcgagg acgcgccggg
     1021 cgagcggcgc cgtcgtgttc gtcgcgaccg aggtccggat cccccggcgg cgaagctccg
     1081 cgagcacggg gacggcctcg gcgttcggcc ggatctcccc gagatgggcc tcgaagagcc
     1141 gcgcataggc cgcgtcgatc tcctcgacgc gccgctcggg catgtaggcg tcccggtcgg
     1201 ccgccgcgcc gttgccgtag atcgcgtcga attcggcccg cgagacgggc cggtggccga
     1261 agcctgtgag cgtctcgttg aagaccgaga accaggcgtc ctccgagaag acgaggacgc
     1321 cgtccatgtc gaagaggacc gcgcggacgg gagcgggcaa gggcacctcc gggcggcgga
     1381 tcatcgcaga gggagggcgg ccggcgcgct tcagcgcgtg cgtgcgatct cgaacgcatc
     1441 cgcgagacaa cgcgctgccc tccggcatcg cttcccctcg cagccgccgc acggcgcgcc
     1501 gatcgatcgg gcccgtggcc cacggagccc ggcgcgggcg ggtttcgtcg gtccgaggat
     1561 cgacagcgac tcggcgccga ggagggccgc catgtgcccg ggccccgtgt ccgccgagac
     1621 gacccgctcc gccgcgaggg cgagccccag gctccccagg agcggcatcg ccccggcgca
     1681 gttcaggatc ccctcccgcc cgggccgatg atccgcgagc cgttcgtctc cgaggaggac
     1741 gagcggcggg ccgggccagc tctcgagcca ctccggcggg atcgaccggg cctgcccgga
     1801 tgacatcggg aacgcggccg tgaaccgccg cgggaggccg agccgctcgg cttcccggga
     1861 cccggcggcc cgcaggcgcg cggccagcgg ggcgagtccg cgctgcgcct cctcccagcc
     1921 ttcccagacc gacgccccga gcgcctcgac cgcgcgagcc gtgttctcga tcgcgtgggg
     1981 cgtccgccgg cggtcgtaca tcggcacccg gcggtgcagc cacggccagc ggccccgacc
     2041 gacgcggtga tccgccccgc tccagacggt ccagagcgag gagcggagga gcccctggag
     2101 gtcgacgacg agctcgtagc gccgcgcgac gaggagcgcg gcgacgcgct ctcgctcggc
     2161 gaagagctcg gcccagcccg ccgagaaggc ccgccggacg tccacgacga ccgtctccgc
     2221 gacccacggc agctcggcga cgaacggcgc cgccggcggc tccacgagcc agtcgatctc
     2281 cgcgcgcggg aagcgccgcg caagggccgc caccgacggg acggcgtgcg cgacgtcccc
     2341 gagagaggag agcttgacga cgaggagcct caagggcctc attttcgcct cgcgggcggc
     2401 aggcgtcgtc gacctctcgg ggtaacctcg gccgtgcccc gcgtcgccgt cgtcatcgcc
     2461 acgcatcgcc gtccggcgct cctcgccgag gcgctgtcct ccgtcgcggc gcagacgttc
     2521 cgcgacgtcg agacgctcgt cgcggaagac ggcggcaccg aggaaaccgc gcgcgtcgtc
     2581 gccgcgtcgg gcgcgcccgg cgcccggcac gttcccctcg ccttctgcgg aaggcccggc
     2641 cccgtgcgaa acgaggccct ccgccgcgtc tcgtcccccc tcgtcgcgtt cctcgacgac
     2701 gacgacctct ggcttccccg caggctcgag gcacacgtcg tcgctctcga ggccgttccg
     2761 gccgccggcc tcgtcttcgg ccccgtgcgg cgcttcggtg acgcggaggg cacgtggccc
     2821 cggagcgttc ccccgcgcgt cgacctcgcc cggctcctgc gggggaactg cgtcccgctc
     2881 tcctccgtcc tcgcgcgccg ggaagcgctc gagcgggccg gtctctttcc cg
//
LOCUS       DAOTEZ010000501         2900 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_26991_length_2900_cov_5.740598, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000501 DAOTEZ010000000
VERSION     DAOTEZ010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26991_length_2900_cov_5.740598"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="P5164_18440"
     CDS             complement(<1..667)
                     /locus_tag="P5164_18440"
                     /inference="COORDINATES: protein motif:HMM:NF036509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45917.1"
                     /translation="MTTNTPESRVLAALSPRGQRILSFRDLAKGLLGPSADEASLEAL
                     RAEVDALERKGLVVRTRGEKLSLIEFTNLKAGRIAFRGEGRAWLLSGRPGVPDLPIAR
                     GGGGSALDGDFVLVRVEKSKGHAGYRGPAEVGVVVKVLVRRRETVVGRIQRRPDGVVL
                     VPYDTKIDALLRIPDGMDLDAPDGIYVDARITTWPDEHRLAQAEVTELIGFPGEEGVD
                     VEVV"
     gene            complement(726..815)
                     /locus_tag="P5164_18445"
     tRNA            complement(726..815)
                     /locus_tag="P5164_18445"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(779..781),aa:Leu,seq:cag)
     gene            complement(903..1244)
                     /locus_tag="P5164_18450"
     CDS             complement(903..1244)
                     /locus_tag="P5164_18450"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRY45918.1"
                     /translation="MIILRERRERTTVLRIQGVVKLGESARQFSELLEKVLEEDEGPV
                     LLDFEKIDTLDSTGLGELIGYLQRFEERKRKMAIVRPKDRVVALLKLTRLDTLIRIFP
                     DDEAALAYLSG"
     gene            complement(1241..1954)
                     /locus_tag="P5164_18455"
     CDS             complement(1241..1954)
                     /locus_tag="P5164_18455"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRY45919.1"
                     /translation="MTSPGGIRHIALAGPGSAWIARAEEVLDQAGYLVTRVETPEALD
                     AIPSVPAVELVVLDEAFGPEGGLRAARQLRSVPRWRSVSIMVVVPSGSTHLEECLVSG
                     INDFLLAPFPEGELLDKARRLTEIAARREVNTMLRVHEVREAGVTVHGKTLNVSVNGL
                     LAEVDAELSVGRVVDVEFYLPDDPASVRSRGQVARRAYEASTFHPVFGLRFLELSARD
                     RTRIERYVASRERSGEKPA"
     gene            complement(1951..2361)
                     /locus_tag="P5164_18460"
     CDS             complement(1951..2361)
                     /locus_tag="P5164_18460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45920.1"
                     /translation="MTPVPEPLPHAYPFRFVERTVEKRGPAEGRVRALVTVNGRRTEA
                     GEYLPPLTLVEVVAQAALLLEGGDPGIGRTGFLAGVSDFEFDREAGSGDLLDVDVRIA
                     GVLGPIVRFEGTVTDGEGRRVCSGAVTVKKGSPA"
     gene            complement(2358..2627)
                     /locus_tag="P5164_18465"
     CDS             complement(2358..2627)
                     /locus_tag="P5164_18465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006282146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HRY45921.1"
                     /translation="MEQVEELKGRVKSLIVRQLKLEIDPATIKDDAPLFGDDPSGLGL
                     DSIDALELVLGIEKEFGIKVQDEEVGVKAFASVDALCEFVRSKAA"
     gene            complement(2693..>2900)
                     /locus_tag="P5164_18470"
     CDS             complement(2693..>2900)
                     /locus_tag="P5164_18470"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY45922.1"
                     /translation="VNCVAPGFVETEMISFLSKEAREDFTKRIPVRRFGVPEDVAPLV
                     AFLCSDGAAYVSGQTIRVDGGFVG"
BASE COUNT          420 a         1015 c         1058 g          407 t
ORIGIN      
        1 cgacgacctc gacgtcgacc ccttcctcgc ccgggaagcc gatcagctcg gtcacctccg
       61 cctgcgcgag ccggtgctcg tcgggccagg tggtgatccg ggcgtcgacg tagatgccgt
      121 cgggggcgtc gaggtccatg ccgtcgggga tccggaggag ggcgtcgatc ttcgtgtcgt
      181 acgggacgag gacgacgccg tcggggcgcc tctggatccg gccgacgacg gtctcccgcc
      241 gccgcacgag gaccttgacg acgacgccga cctcggcggg gccgcggtag cccgcgtgcc
      301 ccttcgactt ctcgaccctc acgaggacga agtcgccgtc gagcgccgag ccgccacctc
      361 cccgggcgat ggggaggtcg gggacccccg gtctgcccga gaggagccag gcgcgcccct
      421 cgccccggaa cgcgatccgg cccgccttca ggttcgtgaa ctcgatgagg gagagcttct
      481 cgccgcgggt gcggacgacg aggcccttgc gctcgagggc gtcgacctcg gcgcggaggg
      541 cctcgaggga ggcctcgtcg gccgaggggc cgaggagccc cttcgcgagg tcgcggaacg
      601 agaggatgcg ctggccgcgc ggcgagaggg ccgcgaggac gcggctttcg ggggtgttcg
      661 tggtcacggt gtcctttccg gggggcccgc cgcggcaggc ctccgccgcg cggggcgggg
      721 aggagtggtg cgaaaggggg gactcgaacc cccacggctt gttaagagcc acaaggtcct
      781 gagccttgcg cgtctaccag ttccgccact ttcgcacgag cggggagcgg gaggatacga
      841 cgcggccctt tcggcgtcaa acacgcgaga ctccggaacg cggccggaac gcccgcccgc
      901 ggtcaccccg aaagatacgc gagcgcggcc tcgtcgtccg ggaagatccg gatcagggtg
      961 tcgagccgcg tcagcttcag gagcgcgacg acgcggtcct tcgggcggac gatcgccatc
     1021 ttcctctttc gctcctcgaa gcgctggagg tagccgatca gctccccgag gccggtggag
     1081 tcgagcgtgt cgatcttctc gaagtcgagg agcaccggcc cctcgtcttc ctcgagcacc
     1141 ttctcgagga gctcggagaa ctggcgggcg ctctccccca gcttcacgac cccctggatc
     1201 cggaggacgg tcgtcctctc gcggcgttcg cggaggatga tcacgcgggc ttctccccgg
     1261 accgctcgcg cgaggcgacg tagcgctcga tccgcgtccg gtcgcgcgcg gagagctcga
     1321 ggaagcggag gccgaagacg gggtggaacg tcgacgcctc gtacgctcgc cgcgcgacct
     1381 ggccgcggct ccgcaccgag gcagggtcgt cggggaggta gaactcgacg tcgacgacgc
     1441 gcccgacgct cagctcggcg tccacctccg ccaggagccc gttcaccgag acgttgagcg
     1501 tcttgccgtg gaccgtgacg cccgcctccc ggacctcgtg gacgcggagc atcgtgttca
     1561 cctcgcggcg cgcggcgatc tcggtgaggc ggcgcgcctt gtcgaggagc tcgccctcgg
     1621 ggaagggggc gaggaggaag tcgttgatcc cggagacgag gcactcctcg aggtgcgtcg
     1681 agcccgaggg gacgacgacc atgatcgaga cggacctcca ccgcgggacc gaccggagct
     1741 gccgggcggc gcggagcccc ccctcggggc cgaacgcctc gtcgaggacg acgagctcga
     1801 cggcggggac ggacgggatg gcgtccagcg cctcgggggt ctcgacgcgc gtcacgaggt
     1861 agccggcctg gtcgaggacc tcctccgcgc gggcgatcca cgccgatccc ggccccgcga
     1921 gggcgatgtg ccggattccg ccgggagagg tcacgccggg cttcccttct tcacggtgac
     1981 ggccccggag cagactcttc gcccctcgcc atcggtgacg gtcccctcga agcgcacgat
     2041 cgggccgagg acgccggcga tccggacgtc gacgtcgagc aggtcgccgc tcccggcttc
     2101 cctgtcgaac tcgaagtccg agaccccggc gaggaagccg gtccggccga tgcccggatc
     2161 gcccccctcg aggaggagcg ccgcctgcgc gacgacctcg acgagcgtga gagggggaag
     2221 gtactccccc gcctccgttc gccgtccgtt cacggtcacg agcgcccgga cccgtccctc
     2281 cgcggggccg cgcttctcca cggtccgctc gacgaagcgg aacgggtacg cgtgcgggag
     2341 gggctcggga acgggtgtca cgccgccttc gaccggacga actcgcagag ggcgtcgacg
     2401 gaggcgaagg ccttgacccc gacctcctcg tcctggacct tgatgccgaa ctccttctcg
     2461 atgccgagga cgagctcgag ggcgtcgatc gagtcgagcc ccaggccgct cgggtcgtcc
     2521 ccgaagaggg gtgcgtcgtc cttgatcgtc gcggggtcga tctccagctt cagctggcgg
     2581 acgatcagcg acttgacgcg ccccttcagc tcttcaacct gctccattcc gcctccttcc
     2641 gggcgatggc cgggagtata tcgagggcga cgtcccgggg aaggcgcgcg gctcagccga
     2701 cgaagccgcc gtcgacgcgg atcgtctggc cggagacgta cgccgcgccg tcggagcaca
     2761 ggaacgccac gagcggggcg acgtcctccg ggactccgaa gcgccggacg gggatccgct
     2821 tcgtgaagtc ctcgcgggct tccttcgaga ggaaggagat catctcggtc tcgacgaagc
     2881 cgggcgcgac gcagttcacc
//
LOCUS       DAOTEZ010000502         2899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_27007_length_2899_cov_3.793249, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000502 DAOTEZ010000000
VERSION     DAOTEZ010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27007_length_2899_cov_3.793249"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /gene="murB"
                     /locus_tag="P5164_18475"
     CDS             <1..682
                     /gene="murB"
                     /locus_tag="P5164_18475"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRY45923.1"
                     /translation="AVSVEAPLVRAGGGASLMSLAVTARNAGLSGTEGLSGIPSSVGG
                     AVRINAGAYGAEIFDLLEEVTLVSRAGALRVVSASTIPHGYRWSALCEGDDVVASAML
                     RLRPAPREEIDARLAEVRERRKKALPTEPNAGSVFKNPPGDHAGRLLEACGLKGARVG
                     GAVVSERHANVIVNAGGATAADVRALMARMREVVRDRFGVDLVPEVEDLGLAVTNRND
                     AASVESGR"
     gene            679..1731
                     /locus_tag="P5164_18480"
     CDS             679..1731
                     /locus_tag="P5164_18480"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRY45924.1"
                     /translation="MTVRALILAGGSGTRLWPLSTDDRPKPFLPLSGKASLLRETHER
                     AARLCGESNVLYSARASHAPHLAKEFPGLPPTRLVLEPERRNTAPAIALAALGVADAD
                     PDGVLVVLPSDQAVRDVDELHRALSLAVESARSGDAFLTLGIPPTRPETGFGYLEAGR
                     ETTPGVREVVRFVEKPPLADAERYAVSGNHYWNAGIFAFSVRLLFSEMERLCPDVLAS
                     MRAAHAARRAGDDAAFEAAFRACRSISIDYAVMEKATAVRTVPSSCGWSDLGSWEAVL
                     EFRGGADGRSVLSGPAIEEGGSGNLVLAGDRPVRLLGLSDVVVVDAPDGLLVMKRGGS
                     DLLRASVEKSLAKGRT"
     gene            1728..2690
                     /locus_tag="P5164_18485"
     CDS             1728..2690
                     /locus_tag="P5164_18485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRY45925.1"
                     /translation="MSAAVPDVVLPAVSRLVSAVRTVYRGPAIAVEHAVVCLLSRGHL
                     LIEDVPGVGKTTLAMALGKALGLGVHRLQFTPDTLPSDILGLTIFNQQSQAFEFHPGP
                     IFTPILVADEINRATPKAQAALLEAMNERSVTVDGETRPLPEPFLVLATQNPIEYLGT
                     YPLPESQLDRFLMRISLGYPAVEEERRLLISGGADRALASVRPVLTLDELKRAQEAVE
                     RVTVAEKLLDYLMALVQKTRSTRDLVLGVSPRGAQGFFRAVQAHALVAGRPFATPDDV
                     KRVAPAVLAHRILASATGRYEGTGAGRREREALQKILDEVAVPL"
BASE COUNT          379 a         1077 c         1028 g          415 t
ORIGIN      
        1 ggcggtgtcg gtcgaggctc ctctcgtccg cgccggcggc ggcgcctcgc tcatgtcgct
       61 cgcggtcacg gcgcggaacg ccggcctgtc ggggaccgag gggctctccg ggatcccgtc
      121 ctcggtcggc ggggccgtgc ggatcaacgc cggggcgtac ggggcggaga tcttcgacct
      181 cctcgaggag gtgacgctcg tctcgcgcgc gggagcgctc cgcgtcgttt cggcctcgac
      241 gatcccgcac ggctaccgct ggtcggcgct ctgcgaaggg gacgacgtcg tcgcctcggc
      301 gatgctccgc ctgcgccccg cgccgcggga ggagatcgac gcgcggctcg ccgaggtgcg
      361 ggagaggcga aagaaggctc tcccgacgga gccgaacgcc ggctcggtct tcaagaaccc
      421 ccccggcgac cacgcggggc ggctcctcga ggcgtgcggg ctgaaggggg cgcgggtcgg
      481 cggagccgtc gtctcggagc gccacgcgaa cgtgatcgtg aacgccggcg gcgccaccgc
      541 ggccgacgtg agggccctga tggcccggat gcgcgaggtg gtccgcgacc gattcggggt
      601 cgacctcgtt ccggaagtgg aagacctcgg gctcgccgtc accaaccgga acgacgccgc
      661 ctccgtagaa tcgggacgat gacggtccgc gcgctgatcc tcgccggcgg gagcggcacc
      721 cgcctctggc ccctctccac cgacgaccgc ccgaagccgt tcctcccgct ctcggggaag
      781 gcctccctcc tgcgggagac gcacgagcgc gccgcccgcc tctgcggcga gtcgaacgtc
      841 ctctattccg cccgggcctc gcacgcgccc cacctggcga aggagttccc ggggctgccg
      901 ccgacgcgac tcgtcctcga gccggagcgg cggaacaccg cgccggcgat cgccctcgcc
      961 gcgctcggcg tggcggacgc ggaccccgac ggcgtgctcg tcgtcctccc gtccgaccag
     1021 gccgtccggg acgtcgacga gctccaccgc gccctctccc tcgccgtgga gagcgcccgc
     1081 tcgggcgacg cgttcctgac gctcggcatc ccgccgacgc gacccgagac cggattcggc
     1141 tacctcgagg cggggaggga gacgaccccc ggagtccgcg aggtggtccg cttcgtcgag
     1201 aagccgccgc tcgccgacgc cgagcgctac gccgtctccg ggaaccacta ctggaacgcc
     1261 gggatcttcg cgttctccgt ccgcctcctc ttctcggaga tggagcgcct ctgccccgac
     1321 gtcctcgcct cgatgcgcgc ggcccacgcg gcgcggcggg caggggacga cgcggcgttc
     1381 gaggccgcgt tccgcgcctg ccggtcgatc tcgatcgact acgcggtgat ggagaaggcg
     1441 acggccgtcc ggacggttcc ctcctcctgc ggctggagcg acctcgggtc gtgggaagcc
     1501 gtcctcgagt tccgcggggg cgccgacgga cgttccgtcc tctccggtcc ggcgatcgag
     1561 gaggggggct ccgggaacct cgtcctcgcg ggcgaccggc cggtccgcct cctcggcctc
     1621 tccgacgtcg tcgtcgtcga cgcgccggac ggcctcctcg tcatgaagcg gggaggctcg
     1681 gacctcctcc gcgcgagcgt ggagaagagc ctcgcgaagg ggcggacgtg agcgcggcgg
     1741 tcccggacgt cgtcctgccg gcggtctcgc gcctcgtctc ggccgtccgg acggtctatc
     1801 gcggtccggc gatcgcggtg gagcacgccg tcgtctgcct cctctcccgc gggcacctcc
     1861 tgatcgagga cgtgccgggc gtcggaaaga cgacgctcgc gatggcgctc ggcaaggcgc
     1921 tcggtctcgg cgtccaccgg ctccagttca cgcccgacac gctcccgtcc gacatcctcg
     1981 gcctgacgat cttcaaccag caatcgcagg cgttcgagtt ccacccgggg ccgatcttca
     2041 cgccgatcct cgtcgccgac gagatcaacc gcgcgacgcc gaaggcgcag gctgccctgc
     2101 tggaggcgat gaacgagcgg agcgtgacgg tggacggcga gacccgtccg ctgcccgagc
     2161 ccttcctcgt cctcgcgacg cagaacccga tcgagtacct cggcacgtac ccgctccccg
     2221 agtcgcagct cgaccgcttt ctcatgcgga tctcgctcgg ctacccggct gttgaggagg
     2281 aaaggcggct cctcatctcg ggaggtgccg accgggcgct cgcctccgta cggcccgtcc
     2341 tgaccctcga cgagctgaag cgggctcagg aggcggtgga gcgggtgacc gtggcagaga
     2401 agctcctcga ctacctgatg gccctcgtcc agaagacccg gtcgacccgc gacctcgtcc
     2461 tcggcgtctc gccgcgaggt gcgcagggat tcttccgggc agtccaggcg cacgcactcg
     2521 tggcggggcg ccccttcgcc acgcccgacg acgtgaagag ggtcgcgccg gccgtcctcg
     2581 cccaccggat cctcgcgagc gccaccgggc gctacgaggg aacgggcgcc ggccgccgcg
     2641 agcgcgaggc gctccagaag atcctggacg aggtcgccgt cccgctctga cgggagggcg
     2701 cgcggaacgc atggtcgagg cagaggcgtc gtcgaggtag atgcaccgtg aagctgtcgg
     2761 tcctcaggct tcggctgaag atcccgtggg acgacaagat cctcgtccgg gtcacgaacc
     2821 tcggcctggg tttcgtcttc acgacgctcg tcgtcgccat cggcgcgacg aacacgggga
     2881 acaacggcct ctacgtcct
//
LOCUS       DAOTEZ010000503         2882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_27255_length_2882_cov_7.716661, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000503 DAOTEZ010000000
VERSION     DAOTEZ010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27255_length_2882_cov_7.716661"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="P5164_18490"
     CDS             complement(<1..410)
                     /locus_tag="P5164_18490"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HRY45926.1"
                     /translation="MTLHRRRPGTAVLALLAVLALPASAEVLEQILVHVNGRIVTQSQ
                     LDDRVRAVLAEAPPDLDQGRRDDIRRAALKELVDESLLEDRARDLDLVATDAEVEEQV
                     KHLKEQNGIASEEEFVRALAASSLTPARLREQLKR"
     gene            complement(407..1273)
                     /locus_tag="P5164_18495"
     CDS             complement(407..1273)
                     /locus_tag="P5164_18495"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRY45927.1"
                     /translation="MRRAAILLALLVTAPLAVPGCARKGPRAATRASVVAMVGDEPVE
                     EEDFENYVRAAAGAGLGQVSPQVASSLLDQYLDERLLERAVAEAEPPPSGKTAAGRRA
                     EVLARAARLEEITDADLRAEYDAEPARWEHPPLVRISQMILPDRAAAETARRRVLAGE
                     AWEKVSREVSRAPNAVSGGGLGWLSRTDLPGEFEKAIWGLPAGGVSPPQPAGHGVHLF
                     RVEERADGSVVPFEEARPALRLALSEKRSSEALERLLADVRKRHRVAVIEEHLPFPYV
                     GGAARFSLDSAP"
     gene            complement(1270..>2882)
                     /locus_tag="P5164_18500"
     CDS             complement(1270..>2882)
                     /locus_tag="P5164_18500"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRY45928.1"
                     /translation="RLLCGDVGYGKTEVAMRAVFKCVLDGKQAAILAPTTILADQHHR
                     TLRRRFAAFPVTIDLLSRFRSSDEQKEVVRRLAEGTLDVVVGTHRILSKDVSFKDLGL
                     VVIDEEQRFGVAQKEKLKELRASVEVLSMSATPIPRSLNLALGGIRDLSVIETPPKDR
                     LAISTHVVPQGDEVVREAIRAELDRGGQVYLVHNRIEGLGVWRERLAELVPECRVVVG
                     HGQMSEGELERTMRAFTTRAADLLLATTIVENGLDIPTANTMIIDRADLYGLSQLYQL
                     RGRVGRSDKPASCWLLVPPGAPLSDDARRRLRAIQEFSDLGAGFRIAAKDLEIRGAGN
                     VLGGEQSGHIDAVGFETYVSLLEEAIGELKGEPVAEKREVTLQLGLPLGLPKKWIGEE
                     SLRMALYKRLATADGIDALDLLEKEATDRYGAPPPEFARLLGLGRLRLLALDLGVRAL
                     QRRGAELAVTLDKGHRLDPDRLLGAIRAKSLAASGPESFRAPELFRGLPPDAEEVCGV
                     AGRFLVSLARPAAAFAPGLLAAFGGGPR"
BASE COUNT          389 a         1051 c         1059 g          383 t
ORIGIN      
        1 cgcttcagct gctcgcgaag ccgcgcggga gtgaggctgg aggcggccag cgcccggacg
       61 aattcctcct ccgacgcgat gccgttctgc tccttgaggt gcttcacctg ctcctcgacc
      121 tcggcgtcgg tcgcgacgag gtcgaggtcc cgcgcccggt cctcgaggag cgactcgtcg
      181 acgagctcct tgagcgcggc gcgccgaatg tcgtcgcggc gcccctggtc gaggtccggc
      241 ggagcctcgg cgaggaccgc gcgcacccgg tcgtcgagct ggctctgcgt gacgatccgc
      301 ccgttgacgt ggacgaggat ctgttcgagg acctcggcgc tcgcggggag cgcgaggacg
      361 gcgaggaggg cgaggacggc ggtgccggga cggcgtcggt ggagcgtcac ggcgcggagt
      421 ctagcgaaaa gcgggccgcc cccccgacat acgggaacgg gaggtgctcc tcgatgaccg
      481 cgacgcggtg ccgtttgcgg acgtcggcga ggagccgctc gagcgcttcg ctgctccgtt
      541 tctcggaaag cgccagccgg agggccggac gggcctcctc gaacggaacg acgctcccgt
      601 cagctcgctc ctcgacccgg aagaggtgga cgccgtgccc ggccggctgg ggcgggctca
      661 cccccccggc cggaaggccc cagatcgcct tctcgaactc gcccggaaga tcggtccgcg
      721 agagccagcc gagccctccg cccgacaccg cgttcggggc gcggctcacc tcccgggaga
      781 ccttctccca ggcctctccc gcgaggacac ggcgccgcgc cgtctccgcg gcggcccgat
      841 cggggaggat catctgcgag atcctcacga gcggggggtg ctcccaccgc gccggctccg
      901 cgtcgtactc ggcccggagg tcggcgtcgg tgatctcctc gaggcgcgcg gcccgggcga
      961 ggacctcggc ccgcctcccg gccgccgtct tccccgacgg cggcggctcc gcctcggcga
     1021 cggcccgctc gaggaggcgc tcgtcgaggt actggtcgag aaggctcgag gcgacctggg
     1081 gggagacctg cccaaggccg gcccccgcgg ccgcgcggac gtagttctcg aagtcctcct
     1141 cctccaccgg ctcgtcgccg accatcgcca cgacggaggc ccgcgtcgcg gcccgaggcc
     1201 ccttccgggc gcagccggga acggccaggg gcgccgtgac gaggagggcc aggaggatcg
     1261 cggcccgcct caacggggac ctcccccgaa ggcggcgagg agcccgggcg cgaaggccgc
     1321 ggcgggccgc gcgagggaga cgaggaagcg tcccgcgacg ccgcagacct cctccgcgtc
     1381 gggaggaagc ccccggaaga gctccggcgc gcggaacgac tccggccccg aagccgcgag
     1441 actcttcgcc cggatcgccc cgaggagccg gtcgggatcg aggcggtgcc ccttgtcgag
     1501 cgtcacggcg agctcggcgc cacgccgctg gagcgcgcgg acgccgaggt cgagcgcgag
     1561 gagccgcagg cgcccgaggc cgaggagccg cgcgaactcg ggcggcggcg cgccgtaccg
     1621 gtccgtcgcc tccttctcga ggaggtcgag ggcgtcgatc ccgtcggccg tggcgagccg
     1681 cttgtagagc gccatccgaa ggctctcctc tccgatccac ttcttcggca ggccgagcgg
     1741 caggccgagc tgaagcgtca cctcccgctt ctcggcgacc ggctcgccct tcagctcgcc
     1801 gatcgcctcc tcgagaagcg agacgtacgt ctcgaacccg acggcgtcga tgtgtcccga
     1861 ctgctcccct ccgaggacgt tccccgcgcc ccggatctcg aggtccttcg ccgcgatccg
     1921 gaatcccgcc ccgaggtccg agaactcctg gatcgcccgg aggcgccggc gcgcgtcgtc
     1981 gctcagcggc gcgccgggcg ggacgaggag ccagcaggaa gccggcttgt cgctccggcc
     2041 gacgcgcccg cggagctggt agagctgcga gaggccgtac aggtcggccc ggtcgatgat
     2101 catcgtgttc gcggtcggta tgtcgaggcc gttctcgacg atcgtcgtcg ccaggaggag
     2161 gtcggcggcg cgcgtcgtga aggcgcgcat cgtccgctcg agctcccctt ccgacatctg
     2221 cccgtggccg acgacgacgc ggcactccgg aacgagctcg gcgagccgct cgcgccagac
     2281 gccgagcccc tcgatccggt tgtggacgag gtagacctgc ccgccccggt cgagctcggc
     2341 gcggatcgcc tcccgcacga cctcgtctcc ctgcgggacg acgtgcgtcg agatcgcgag
     2401 gcggtccttc ggcggcgtct cgatgaccga gaggtcgcgg atccctccga gcgcgaggtt
     2461 cagcgagcgc gggatcggcg tcgccgacat cgagaggacc tccaccgagg cgcgcagctc
     2521 cttcagcttc tccttctggg cgacgccgaa gcgctgctcc tcgtcgatga cgacgaggcc
     2581 gaggtccttg aaggagacgt ccttcgagag gatccggtgc gtcccgacga cgacgtcgag
     2641 cgtcccttcg gcgaggcgcc gcacgacctc cttctgctcg tcggacgagc ggaagcggga
     2701 gagcaggtcg atcgtcaccg ggaaggcggc gaagcggcgg cgcagcgtcc ggtggtgctg
     2761 gtcggcgagg atcgtcgtcg gagcgaggat cgccgcctgc ttgccgtcga ggacgcactt
     2821 gaagaccgcc cgcatcgcga cctcggtctt cccgtagccg acgtcgccgc agaggagccg
     2881 gt
//
LOCUS       DAOTEZ010000504         2874 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_27369_length_2874_cov_7.457254, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000504 DAOTEZ010000000
VERSION     DAOTEZ010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27369_length_2874_cov_7.457254"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /locus_tag="P5164_18505"
     CDS             <1..1320
                     /locus_tag="P5164_18505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRY45929.1"
                     /translation="CDILIGTPGRLLDYEGQGATSFGSVESLVIDECDRLFDMGFLPD
                     LRRILRRCPPPRRRQSMMFSATLSWKVTELAWEHMNEAVRVEIAPEHVTADRVVQSLF
                     HVGNSEKLSLLVGLLQREGNATRTMLFVNTKRFAERLVERLERHGFRCGAISGDIPQA
                     KRLKILAEFKSGKLPILVATDVASRGLHIEGVTHVINVDLPMDPEDYVHRIGRTARAG
                     HSGRAISLACENYVQSLSSIEKLIGMKIPVEHPDDALFVRRPAPLREPVRVSGGPRSA
                     APAAGDEDADAKAPPPPRASRPRRERAAAPARPEPAPAAPAPRVEKPQGPAVASPAPA
                     VPDVPVAPAVLAEAPVLVAKDAPPAVAEVGTREANVRVEPAADLPAAEEPSPGDVAPT
                     DFWTCAAFELEIPEGGFGVSASPAAPGGASRRKRHRRRKPASAAPAS"
     gene            1369..2178
                     /locus_tag="P5164_18510"
     CDS             1369..2178
                     /locus_tag="P5164_18510"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRY45930.1"
                     /translation="MPFAGVRDQRFYYEVGGEGELVILLHGALANADLMEAPATGLAS
                     GFRALRFDRRGCGRTTPPTEGPVPLPEEADDVLGILDWFSVEKAHFLAHDEGAEVALE
                     FALRNPGRTGSIALLAPSLEGFSSSPEVAAASADLRAALRSDPPKALAEKLFPSPIFD
                     AAREREGVFERITDIFRRFPQSPGRFERLPRAGAALAGRLGAVSARTFVLVGERDEED
                     RLRSARTIAAGIPGAELLVFPDTSRFLHVEESRAVMRRLTDFFLPEPEIEP"
     gene            complement(2329..>2874)
                     /locus_tag="P5164_18515"
     CDS             complement(2329..>2874)
                     /locus_tag="P5164_18515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020475780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRY45931.1"
                     /translation="AHHIAVAEATFERAKRLVEIGKDVVIFCDSLTRMSRAYNNEQRG
                     SGKILSGGIDARTMEKPRRFFGGARNHEDGGSLTIIATCLVDTGSRMDDVIFEEFKGT
                     GNCEIILDRGLFDRRVFPCINIPASGTRKEEKLYTAEELPKIHKLRRALSSVKPMDAM
                     ELLLSKLTRTKTNAEFLKSIA"
BASE COUNT          369 a         1099 c          972 g          434 t
ORIGIN      
        1 tgcgacatcc tgatcggcac ccccggccgg ctcctcgact acgaggggca gggggcgacc
       61 tcgttcgggt cggtcgagtc gctcgtcatc gacgagtgcg accgcctctt cgacatgggg
      121 ttcctccccg acctccggcg catcctccgg cgctgccccc cgccgcgccg gcgccagtcg
      181 atgatgttct cggcgactct ctcctggaag gtcacggagc tcgcttggga gcacatgaac
      241 gaggccgtcc gggtcgagat cgccccggag cacgtgacgg ccgaccgcgt cgtccagagc
      301 ctcttccacg tcgggaactc cgagaagctt tcgctcctcg tcggcctcct gcagcgcgag
      361 gggaacgcga cccggacgat gctcttcgtg aacacgaagc gctttgccga gcggctcgtg
      421 gagcgcctcg agaggcacgg cttccgctgc ggcgcgatct cgggcgacat cccccaggcg
      481 aagcggctga agatcctcgc cgagttcaag tcggggaagc tcccgatcct cgtcgccacg
      541 gacgtggcgt cccgcgggct ccacatcgaa ggcgtcacgc acgtcatcaa cgtcgacctg
      601 ccgatggacc cggaggacta cgtccaccgg atcggccgga cggcgcgggc cgggcactcg
      661 ggccgggcca tctccctggc ctgcgagaac tacgtccagt cgctctcctc catcgagaag
      721 ctgatcggga tgaagatccc ggtcgagcat cccgacgacg cgctcttcgt caggcgcccc
      781 gcgccgcttc gcgagccggt tcgcgtctcg gggggccctc gctccgccgc ccccgccgcg
      841 ggggacgagg acgcggacgc gaaggctccg cccccgccgc gggcctcgcg cccgcggcgg
      901 gagcgggccg ccgcacccgc gcgtcccgag cccgcacccg ccgcaccggc cccgcgcgtt
      961 gagaagccgc agggcccggc ggtcgcatcc cccgcgcctg ccgtgcccga cgtccccgtc
     1021 gcgcccgccg tgctcgcaga agcgccggtc ctcgtcgcca aggacgctcc gccggccgtc
     1081 gcggaagtcg ggactcgcga ggcgaacgtc cgcgtcgagc ccgcggccga tctccctgcg
     1141 gccgaggagc cgtccccggg cgacgtcgcg ccgaccgatt tctggacctg cgccgccttc
     1201 gagctcgaga tccccgaggg agggttcggc gtctcggcct cccccgcggc gcccggaggc
     1261 gcctcgcggc gaaagcgcca tcggcggcgc aagcccgcct ccgcggcccc cgcctcctga
     1321 cccccggcgt ctcccgcggc ttatgattcc tcttccggga ggaacgccat gccgtttgcc
     1381 ggagttcggg accagcgttt ctactacgag gtcggaggcg agggagagct cgtcatcctc
     1441 ctccacggcg ccctcgcgaa cgccgacctg atggaggccc ctgcgacggg cctggcgtcg
     1501 gggttccgcg cgctgcgatt cgaccggcgc ggctgcggcc ggacgacgcc gccgaccgag
     1561 ggccccgtgc cgctccccga ggaggccgac gacgtcctcg ggatcctcga ctggttctcc
     1621 gtcgagaagg cccacttcct cgcgcacgac gagggggccg aggtcgccct cgagttcgcc
     1681 ctccgcaacc ccggccgcac cgggtcgatc gcgctgctcg cgccgtcgct cgagggcttc
     1741 tcctcctcac ccgaggtggc ggcggcctcg gcggaccttc gggccgctct ccggagcgac
     1801 cccccgaagg cgctcgcgga gaagctcttt ccctccccga tcttcgacgc ggcccgcgag
     1861 agggaagggg tcttcgagag gatcaccgac atcttccgcc gcttcccgca gagcccgggc
     1921 cgcttcgagc gccttccccg cgccggcgcc gcgctcgccg ggcgcctcgg cgccgtctcg
     1981 gcgcggacgt tcgtcctcgt cggcgagcgc gacgaggagg accggctccg ctcggcgcgg
     2041 acgatcgccg ccgggatccc gggcgcggag ctcctcgtgt tccccgacac ctcccgcttc
     2101 ctccacgtcg aggaaagccg cgcggtcatg cgcaggctga ccgacttctt cctccccgag
     2161 cccgagatcg aaccctgacc cgcccctctc cccccgccca ccgccctcat gcgcgcgcgc
     2221 cgttggcgcg cgggctgcga cgaagatggg agaggggtgc aagcgaacgg tggacaacgc
     2281 gaaacggggg cctcgcggcc cccgtttcgg ttcacgtgct ccctgggact aggcgatcga
     2341 cttcaggaac tcggcgttcg tcttcgtccg ggtcagcttc gagaggagga gctccatcgc
     2401 gtccatcggc ttgaccgacg agagcgcacg ccggagcttg tggatcttcg gcagctcctc
     2461 ggcggtgtag agcttctctt ccttccgcgt tcccgaggcg gggatgttga tgcacgggaa
     2521 gacgcgccgg tcgaagaggc cgcggtcgag gatgatctcg cagttccccg tccccttgaa
     2581 ctcctcgaag atgacgtcgt ccatccggct gccggtgtcg acgaggcagg tcgcgatgat
     2641 cgtgagcgac ccgccgtcct cgtggttgcg cgcgccgccg aagaagcgcc gcggcttctc
     2701 catcgtccgc gcgtcgatgc cgcccgagag gatcttgccg ctcccgcgct gctcgttgtt
     2761 gtaggcgcgg ctcatccggg tgagcgagtc gcagaagatg acgacgtcct tgccgatctc
     2821 gacgaggcgc ttggcccgct cgaacgtcgc ctcggcgacg gcgatgtggt gcgc
//
LOCUS       DAOTEZ010000505         2867 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_27485_length_2867_cov_8.705548, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000505 DAOTEZ010000000
VERSION     DAOTEZ010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27485_length_2867_cov_8.705548"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="P5164_18520"
     CDS             complement(<1..451)
                     /locus_tag="P5164_18520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45932.1"
                     /translation="MSANAPIGARLAAGRRRLPSLSLLAAFLVLLPSCGKMLSPPPQP
                     RTDGYSGLLTVRAGGAELAKFRVDVRGEALRRTTADAKDAPYFVRESAAGPVWEVDPA
                     SKSYREATEEALFAHLDDFPLGADFNHAAEANRRGITKYHRESDAVFA"
     gene            complement(448..1383)
                     /locus_tag="P5164_18525"
     CDS             complement(448..1383)
                     /locus_tag="P5164_18525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45933.1"
                     /translation="MKPRPPRELPAGAYPLPLSTLDWLLSRENPAARYVAFRDLLHRP
                     PKDIERRKARQSLLRDPFLRDALPHLRGMLPVGAPADFAAPVDAGTSFVLLLLEIGCD
                     ADVPELQHAADLFLARWERVFVEIERGESPTVGSGFVAACRALLRLGYAEDDRLHSAA
                     EHLARRRVGSDSASEGVAGDLLVLAGVPEAKRSALLQNAIAFCVERATAVELPALSVE
                     AQKTGIGVGDASDLLEILGALVAAGAPRTPGLDEALGRLAARADHRGRFKLERPVGVP
                     LPIPLEREGELSRWVTLRALVVMQHFLGLTITGVP"
     gene            2490..>2867
                     /locus_tag="P5164_18530"
     CDS             2490..>2867
                     /locus_tag="P5164_18530"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRY45934.1"
                     /translation="MNVFRSGPPLVVARRGSAREAVENTVEAFDLAEAEGADALHLDV
                     RLTADGEVVVAADPEVVVGNQRVAVASLCVPDLLRAELVKGDRRYALPLLRDVLMRYG
                     SDGSYLVELKPGPSPRPGLLEFRV"
BASE COUNT          339 a         1070 c         1149 g          309 t
ORIGIN      
        1 ccgcgaagac ggcgtccgac tcgcggtggt acttcgtgat cccgcgccgg ttggcttcgg
       61 ccgcgtggtt gaagtcggcg ccgagcggga agtcgtcgag gtgcgcgaag agcgcctcct
      121 ccgtcgcctc ccggtagctc ttcgaggccg ggtcgacctc ccaaaccgga cccgccgccg
      181 actcccggac gaagtacggc gcgtccttcg cgtccgccgt cgtccgccga agcgcctcgc
      241 cgcggacgtc gacgcggaac ttcgcgagct ccgccccgcc cgcccggacc gtcagcagcc
      301 cggagtagcc gtccgtcctg ggctgcggcg gcgggctcag catcttcccg caggagggaa
      361 ggaggacgag gaacgcggcg aggagggaga gcgacgggag gcgcctcctc cccgccgcga
      421 ggcgggcgcc gatcggtgcg ttcgcgctca tgggaccccc gtgatcgtca ggccgaggaa
      481 gtgctgcatc acgacgagcg cgcggagcgt cacccaccgg gagagctccc cctcgcgctc
      541 gaggggaatc ggaagaggca cgccgaccgg acgctccagc ttgaagcgcc cgcgatggtc
      601 cgcgcgcgcg gcgagccggc ccagcgcctc gtcgaggccc ggcgtgcgcg gcgcgccggc
      661 cgcgacgagg gccccgagga tctccagcag gtcggacgcg tctccgacgc cgatcccggt
      721 cttctgggcc tccacggaaa gcgcgggaag ctccaccgcc gtggcccgct cgacgcagaa
      781 cgcgatcgcg ttctgcagga gcgccgaccg cttcgcctcc gggaccccgg cgaggacgag
      841 gaggtccccg gccacgccct cggaggcgct gtcggagccg acccggcgcc gcgcgaggtg
      901 ctcggccgcc gagtggagcc ggtcgtcctc cgcgtagccg aggcggagga gggcccggca
      961 ggcggcgacg aagccgcttc ccaccgtcgg cgactcgccc ctctcgatct ccacgaagac
     1021 ccgctcccag cgggcgagga agaggtcggc cgcgtgctgc agctcgggca cgtccgcgtc
     1081 gcagccgatt tcgaggagga ggaggacgaa cgacgtcccg gcgtcgaccg gcgcggcgaa
     1141 atcggccggg gcgccgacgg ggagcatccc gcgcaggtgc gggagcgcgt cgcgcaggaa
     1201 cgggtcgcgc agcaggctct ggcgcgcctt ccgccgctcg atgtccttcg gcgggcggtg
     1261 caggaggtcg cggaaggcga cgtagcgggc ggccgggttc tcgcgcgaaa ggagccagtc
     1321 gagcgtggag agcgggagcg ggtaggcccc cgccgggagc tcgcgcggcg gccgcggctt
     1381 catctcgcgg cgcacctccg cgggcatcgc gtcggcgacg acctccgcgc gcggcgcggg
     1441 acccgtcgcg gggggccggt ccgggcgtgg ccgcggggcg accgtccgct cgcccggtcc
     1501 cgcctcccgc cgcggccgct cgggccgcgg cgggagatct ccgccgcgga acggagggcg
     1561 ctcgggacgc gcgcttcccg gcccgtcctc gcgccggaaa ggcggacggt cggggcgagg
     1621 ccgcggcggc ccgtcgctga aacggcgcgg cggacggtcg tcgcgcaggg aggacgggcg
     1681 ctcgtcgcgc ccgcggaacg gaggtcggtc cgggcgcggc gaacgctcgt cgcgccggaa
     1741 cggcgggcga tcgccgcggg gagccgcgga cctgtcgtcg cgaccccgga agggcggacg
     1801 gtcgggacgc gggaacggcg gacggtcgtc gcgcgaagac ggcgcgcggt cgtcccggcc
     1861 ccggaacggc ggacggtcgc cacgcgggaa ggacggacga tcctcgcgcc ggaacggcgg
     1921 tcgatcgtcg cggccgcgga acgccggacg gtcgtcgcgc gcagacggcg ggcggtcgtc
     1981 ccggccccgg aacggcggac ggtccggccg cgggaacggc gggcggtcgc ctcgcccccg
     2041 agatgccggg cggtcctcgc gcccgccggc ggtccggtcg tcccgccgga acggtggacg
     2101 ctcctcgcgg cggaacggtg gccgatcgtc ccggccgcgg aacgccggcc ggtcgtcgcg
     2161 cggaggcgcg ggccggtcgt cccggccccg gaacggcgga cggtccgcac gcgggaacgg
     2221 cggacggtcg tcgcgccccc ggaagggggg acggtccggg cgcggggagg acgggccgtc
     2281 ggggcggggc ccctgcggcc tgtcgccggg cggcctcggc gcgaaacggc ccggcccgcc
     2341 ctcgccggcg tgccaggtag gacggtcgcc ggtccggaac ggaggacgcg cgccgggccg
     2401 ggccggcggg cgtcctgtgg gccgtcgcgg cggcccggag cgtgagcccc ctcgcggttt
     2461 catcgggcgc gaggataatc cctcgcggca tgaacgtctt ccgatccggc ccgcccctcg
     2521 tcgtcgcccg ccgcggctcg gcccgcgagg cggtcgagaa caccgtcgag gccttcgacc
     2581 tcgccgaggc cgagggagcc gacgcgctcc acctcgacgt ccggctgacg gccgacgggg
     2641 aggtcgtcgt cgcggccgac ccggaggtcg tcgtcggcaa ccagcgcgtc gcggtcgcct
     2701 cgctctgcgt gcccgacctc ctccgcgccg agctcgtgaa gggggaccgg cgttacgccc
     2761 ttccgctcct gcgcgacgtc ctgatgcgct acggctccga cgggagctac ctcgtcgagc
     2821 tcaagccggg cccctcgccg cgcccgggcc tcctcgagtt ccgcgtc
//
LOCUS       DAOTEZ010000506         2860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_27603_length_2860_cov_8.972193, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000506 DAOTEZ010000000
VERSION     DAOTEZ010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27603_length_2860_cov_8.972193"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..1176
                     /locus_tag="P5164_18535"
     CDS             10..1176
                     /locus_tag="P5164_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007281030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRY45935.1"
                     /translation="MKQQIGSQFIEIIQWLDESDDSLVYRFPVYNQEIKMGAQLTVRE
                     NQAALFVNEGKAADLFLPGRYELSTQNIPILTTLRGWKYGFQSPFKAEVYFFNTRTFT
                     DLKWGTTNPVMMRDSDFGMIRIRAFGTYAMKIADPKVFFNTIVGTQGLTTTDEITGQL
                     RSIVLSKLSDAIAESKIPALDIAAKYDELSAFGKEKIAPEFGSFGLDLTKFFIENVSL
                     PEEVEQAIDQRTKLGVLGDRMGQFTQMQAAEAIKIAAANPSGGLAGAGAGLGAGMAIG
                     GVMGQAFQPMMQPQAAPPPAYGAAAPPPAPGAGAPPPPPAPAAPRWSLAIDGKTYGPY
                     TDEAMKQMIAAGQVAPTTLAWHPGAAGWAPLQTFPGFEGTSPGVVSPPPPPPAR"
     gene            1190..2320
                     /locus_tag="P5164_18540"
     CDS             1190..2320
                     /locus_tag="P5164_18540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRY45936.1"
                     /translation="MTPPPPPIPASPPRPETGGEPVSARARKFPCGACGADVVWHPGA
                     SALKCPYCGSETALPTSSDEVVERPLEEALAAPRSLGWGAERKSVRCTKCGATTTFDP
                     GVSASRCAFCATPAVVEAPSETDHVRPAGLLPFSVDRASAAQRFRSWISGLWFRPNDL
                     KEKASVTAMQGVYVPFWTFDALTHNRWTAEAGHRYTVQVSTLVNGRSVMRSETRIRWE
                     PAAGFLEKVFDDVPVPASKGLPPGLARGIEPFPTGGLVPYDPQYLSGFLAEEYAVDLP
                     QAVEAARERMTREIHAACGAQVPGDTFRNLSVSTAWSGLTCKNALLPVWISAYEYGGR
                     PFRFLVNGVTGKVDGNAPFSAAKITLAILAVLAVILAFAAAQ"
     gene            complement(2402..>2860)
                     /locus_tag="P5164_18545"
     CDS             complement(2402..>2860)
                     /locus_tag="P5164_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRY45937.1"
                     /translation="PDANSTLRVTFGRVMGVDPRDGMRYLPQTTLQGVLDKHTGEGEF
                     DAPDGLLEAAKALRAGKKTPYFDEKLGDVPVDFMSDVDTTGGNSGSAVLNAKGELVGL
                     LFDGTLETVASDFLFDTEKTRSIQVDSRYLLWVWDAVEGAKNLLDEVGIR"
BASE COUNT          414 a         1067 c          966 g          413 t
ORIGIN      
        1 ctcgacaaca tcaagcagca gatcggctcg cagttcatcg agatcatcca gtggctggac
       61 gagtcggacg actcgctcgt ctaccggttc ccggtctaca accaggagat caagatgggc
      121 gcgcagctga cggtgcgcga gaaccaggcc gccctcttcg tgaacgaggg caaggcggcc
      181 gacctgttcc tgcccgggcg ctacgagctc tccacgcaga acatccccat cctgacgacg
      241 ctccgcgggt ggaagtacgg cttccagtcc ccgttcaagg cggaggtcta cttcttcaac
      301 acgcggacct tcaccgacct gaagtggggg acgacgaacc cggtcatgat gcgggactcc
      361 gacttcggga tgatccgcat ccgcgccttc gggacctacg cgatgaagat cgccgacccg
      421 aaggtcttct tcaacacgat cgtcgggacg caggggctga cgacgacgga cgagatcacc
      481 ggccagctcc ggtcgatcgt cctctcgaag ctctccgacg cgatcgccga gtcgaagatc
      541 ccggcgctcg acatcgccgc gaagtacgac gagctctcgg ccttcggcaa ggagaagatc
      601 gcccccgagt tcgggagctt cgggctcgac ctgacgaagt tcttcatcga gaacgtctcc
      661 ctccccgagg aggtcgagca ggcgatcgac cagcggacga agctcggcgt cctcggcgac
      721 aggatggggc agttcaccca gatgcaggcg gccgaggcga tcaagatcgc cgcggcgaac
      781 ccttccgggg gcctcgccgg cgccggcgcc ggcctcggcg ccgggatggc gatcggcggc
      841 gtgatggggc aggccttcca gccgatgatg cagccgcagg ccgccccgcc gccggcctac
      901 ggcgccgcgg ctccgccccc cgccccgggc gccggcgcgc ccccgccgcc ccccgcgccg
      961 gccgctccac gctggtcgct cgcgatcgac gggaagacct acggacccta caccgacgag
     1021 gcgatgaagc agatgatcgc cgcggggcag gtcgctccga cgacgctcgc ctggcatccg
     1081 ggcgcagccg ggtgggcccc gctccagacg ttccccggct tcgaggggac gagccccggc
     1141 gtcgtctcgc cgcccccgcc gccgccggcc cgctgaggag gggcgtccgt tgacgccccc
     1201 tcctcccccg atcccggcct cccctccccg cccggaaacg ggcggggagc ccgtttccgc
     1261 ccgcgcgcgc aagttcccgt gcggcgcctg cggcgccgac gtcgtctggc acccgggcgc
     1321 ctccgcgctg aagtgcccgt actgcggaag cgagacggcg ctgccgacct cctcggacga
     1381 ggtcgtcgag cggccgctcg aggaggcgct cgccgcgccg cgcagcctcg gctggggggc
     1441 cgagaggaag agcgtccgct gcacgaagtg cggcgccacg acgacgttcg accccggcgt
     1501 ctccgcctcc cgctgcgcgt tctgcgcgac ccccgccgtc gtcgaggcgc cgtccgagac
     1561 ggaccacgtc cgccccgccg gcctcctccc cttctccgtc gaccgcgcct ccgccgcgca
     1621 gcgcttccgg agctggatct cgggcctctg gttccggccg aacgacctga aggagaaggc
     1681 ctcggtcacc gcgatgcagg gggtctacgt cccgttctgg acgttcgacg ccctcaccca
     1741 caaccgctgg actgccgagg cgggccaccg ttacaccgtc caggtgtcga ccctcgtgaa
     1801 cggcaggtcc gtcatgcgct ccgagacgcg catccgctgg gagccggccg ccggcttcct
     1861 cgagaaggtc ttcgacgacg tcccggtccc cgcctcgaag gggcttcccc ccggcctcgc
     1921 gcgagggatc gagccgttcc cgacgggcgg cctcgtcccc tacgacccgc agtacctctc
     1981 cggcttcctg gccgaggagt acgccgtcga tctcccgcaa gcggtcgagg cggcgcggga
     2041 gcggatgacg cgcgagatcc acgcggcctg cggggcgcag gtcccgggtg acacgttccg
     2101 gaacctctcc gtctcgacgg cctggtcggg cctgacgtgc aagaacgcgc tgctgccggt
     2161 ctggatctcg gcctacgagt acggcggccg gccgttccga ttcctcgtga acggggtcac
     2221 cggaaaggtg gacgggaacg cgccgttctc cgcggcgaag atcacgctcg ccatcctcgc
     2281 ggttctcgcg gtcatcctcg ccttcgccgc cgcgcagtag gcgttccctc gaaaaggaag
     2341 cggcccgcgt ccccccggcg ggggacgcgg gccgctcgag tctccggcgg agccgggcga
     2401 cctaccggat cccgacctcg tcgaggaggt tcttcgcccc ctcgaccgcg tcccagaccc
     2461 agaggaggta gcggctgtcg acctggatcg accgcgtctt ctccgtgtcg aagaggaagt
     2521 cggaggcgac ggtctcgagc gtcccgtcga agaggaggcc gacgagctcg cccttcgcgt
     2581 tcaggaccgc cgagccggag ttgccgcccg tggtgtccac gtcggacatg aagtcgaccg
     2641 ggacgtcgcc cagcttctcg tcgaagtacg gggtcttctt cccggcccga agcgccttcg
     2701 ccgcctcgag gagaccgtcg ggggcgtcga actccccctc gcccgtgtgc ttgtcgagaa
     2761 cgccctggag cgtcgtctgc gggaggtagc gcatgccgtc gcgcgggtcg acgcccatga
     2821 cgcggccgaa cgtgacgcgg agcgtggagt tcgcgtcggg
//
LOCUS       DAOTEZ010000507         2853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_27735_length_2853_cov_5.437098, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000507 DAOTEZ010000000
VERSION     DAOTEZ010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27735_length_2853_cov_5.437098"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="P5164_18550"
     CDS             complement(<1..1067)
                     /locus_tag="P5164_18550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRY45938.1"
                     /translation="MNRTEMAPLEGQVSSPAPGEFDVVVIGGAIAGASTAILLRRRNP
                     GVRVLVVEKSDAFDWKVGESTVEISAYFLTRVLKLYDHLSREHLPKQAFRYWFHNEKV
                     ACLREASEVGPTQLARTPGFQLDRAVLDEHVLKLAAEEGSEVWRPAKVVDVALGGEAG
                     SSVTVEKADGTRATVRARWVVDASGRQALLARKRGGIRPVEGHPTSSIWVRYRGVKDL
                     DGKEVVGTDPADPFVRSVVASRRLATNHFTGWGYWVWFIPLRGGETSVGLVWDTRLVR
                     PEGSTPLEKLASVLSANPLTREMLEHAEPVAGDCRYYGTLPYLVDRFIDHGWTCVGDA
                     GGFLDPFYSPGLDQLSFSVHMR"
     gene            1220..2413
                     /locus_tag="P5164_18555"
     CDS             1220..2413
                     /locus_tag="P5164_18555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45939.1"
                     /translation="MDHARRFAPRCLVVAALLALPAPPAFASGPAPTPPPAASDGRWF
                     DRGMELHEDQRWDEAIAAFRRAIDAGERVGVSSFNVACAYARKGEKDLAFEWLGRAEA
                     AGFELRRHLDDDDLDSLRTDPRFTALKAKARTARSGRSAEKCRSAVVRFDQLSGRGSK
                     DGRALFDVGQDLLSCGELDRAARAFVAASEAGRREGTSLYNAACARALESRNAEALDL
                     LERAVAAGFDGAGHLRRDDDLDGLRAEPRFERIAKDAEALSLGDFPSLGARLLRSQMV
                     AEADEAAARFEAFLRTNPRSGRAWWSIGKVRLTAGQDREAVHAFTRALSLGYRRGATA
                     YDLACAHARLKERDAAFARLFEAIDLGAATADHVEEDDDLWNLRRDPRFEKAVARARA
                     TRGGR"
     gene            2675..>2853
                     /locus_tag="P5164_18560"
     CDS             2675..>2853
                     /locus_tag="P5164_18560"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRY45940.1"
                     /translation="MKLYVGNLSYNTTDATLNDLFAPFGQVESARVITDRDSGTSKGF
                     GFVEMSNSDAQKAMGA"
BASE COUNT          417 a         1040 c         1013 g          383 t
ORIGIN      
        1 cgcatgtgga ccgagaagct cagctggtcg aggccggggg agtagaacgg gtcgaggaag
       61 ccgcccgcgt cgccgacgca ggtccagccg tggtcgatga agcggtcgac gagatagggg
      121 agcgtgccgt agtagcggca gtcccccgca acgggctcgg cgtgctcgag catctcgcgc
      181 gtcagcgggt tcgcggagag gacgctcgcg agcttctcga gcggggtcga cccctcgggg
      241 cggacgagcc gcgtgtccca gacgagcccg acgctcgtct cgcctccgcg gagcgggatg
      301 aaccaaaccc agtagcccca tccggtgaag tggttcgtgg cgagccgacg ggaggcgacg
      361 acgctccgga cgaacggatc ggccgggtcg gtgccgacga cctccttgcc gtcgaggtcc
      421 ttgacgccgc ggtaccggac ccagatcgac gaggtcggat gcccctcgac cggccggatg
      481 ccgccgcgct tgcgggcgag aagcgcctgg cgcccggagg cgtcgacgac ccacctcgcg
      541 cggacggtcg cgcgcgtccc gtcggccttc tccaccgtga ccgagctccc cgcctcgccg
      601 cccagggcga cgtcgacgac cttcgcgggg cgccagacct cgctcccctc ctcggcggcg
      661 agcttcagga cgtgctcgtc gaggacggcc cggtcgagct ggaacccggg ggtccgcgcg
      721 agctgcgtcg ggccgacctc ggacgcctcg cgcaggcagg cgaccttctc gttgtggaac
      781 cagtagcgga aagcctgctt cgggaggtgc tcgcgcgaga ggtggtcgta gagcttcagg
      841 acgcgcgtga ggaagtacgc ggagatctcg acggtcgact ccccgacctt ccagtcgaac
      901 gcgtccgact tctcgacgac gaggacgcgg acgcccgggt tgcggcggcg aagcagaatg
      961 gcggtcgacg cgccagcgat cgcgcctccg atcacgacga cgtcgaactc gcccggagcg
     1021 ggcgaagaga cctgtccttc caggggagcc atttccgtcc gattcatgcg cggatgatat
     1081 tgaatggggt ccgcgggccc cgaagggcgc cgaaccggct acggtgcaat cacttatgcg
     1141 acgtgccccc aggtcccccg cgtcgaacgg cgggacctga ccgggaaaca ccccttgcgc
     1201 cgctccgtat ggagggggca tggaccacgc ccgacgcttc gccccgcgat gtctcgtcgt
     1261 cgccgcgctc ctcgcccttc ccgcgccccc cgcgttcgca tcggggccgg ccccgacgcc
     1321 gcccccggcc gcgagcgacg gccgctggtt cgaccgcggg atggagctcc acgaggacca
     1381 gaggtgggac gaggcgatcg ccgcgttccg ccgggcgatc gacgcgggcg agcgcgtcgg
     1441 agtctcgtcg ttcaacgtcg cctgcgctta cgcgcgcaag ggcgagaagg acctcgcctt
     1501 cgaatggctc ggtcgggccg aggcggccgg gttcgagctc cggcggcacc tcgacgacga
     1561 cgacctcgat tcgctccgca cggacccccg gttcacggcg ctgaaggcga aagcgcgcac
     1621 ggcccggtcg gggcggagcg ccgagaagtg ccggtcggcc gtcgtccgct tcgaccagct
     1681 ttccgggcgg ggatcgaagg acggccgagc cctcttcgac gtcgggcagg acctcctttc
     1741 gtgcggggag ctcgaccgcg ccgcccgggc cttcgtggcg gcctcggagg ccggccggcg
     1801 cgaggggact tcgctctaca acgcggcctg cgcccgcgcc ctcgagagcc ggaacgccga
     1861 agccctcgac ctcctcgagc gcgccgtcgc cgcgggtttc gacggggccg ggcacctgcg
     1921 gcgcgacgac gacctcgacg gactccgcgc cgagcctcgc ttcgagagga tcgcgaagga
     1981 cgccgaggcg ctctccctgg gggacttccc gagcctcggg gcccggctcc tccgctccca
     2041 gatggtcgcg gaggccgacg aggccgcggc gcggttcgag gcgttcctgc ggacgaaccc
     2101 gcggagcggg cgggcgtggt ggagcatcgg caaggtccgc ctgaccgcgg gccaggaccg
     2161 ggaggccgtc catgccttca cccgcgccct ctccctcggc taccggcgcg gcgcgacggc
     2221 ctacgacctg gcctgcgccc acgcgcgtct gaaggagcgc gacgcggcgt tcgcccgcct
     2281 gttcgaggcg atcgacctcg gcgcggccac cgccgaccac gtcgaggagg acgacgacct
     2341 ctggaacctc cggcgcgacc cccgcttcga gaaggcggtc gcccgggcgc gggcgactcg
     2401 gggcggacgg tgaggagact cggttctctc ccggccggga acggccggcc ccgcgaggga
     2461 gccgtccccc gggagggctg tggccgcccc tccgcgaagt cgtgatattc tgcctctggc
     2521 ccattcgcca ctctccgcct cctcgctctc cgaacttcgg gcagacggcg cggcaaagcg
     2581 taacgagcag gccccgtctc ccgcgcgctt ccgactcacc tatctcccca ccagatcgtc
     2641 ttcggacgac cgagacaaga cacgaggact cacgatgaag ctctacgttg gaaacctctc
     2701 ctacaacacg accgacgcca ccctcaacga cctgttcgcg ccgttcggcc aggtcgagtc
     2761 cgcccgcgtc atcaccgacc gggacagcgg cacctcgaag ggcttcggct tcgtcgagat
     2821 gtcgaactcc gacgcgcaga aggcgatggg cgc
//
LOCUS       DAOTEZ010000508         2850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_27784_length_2850_cov_5.562791, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000508 DAOTEZ010000000
VERSION     DAOTEZ010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27784_length_2850_cov_5.562791"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="P5164_18565"
     CDS             <1..287
                     /locus_tag="P5164_18565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRY45941.1"
                     /translation="DARMTAHVNGIQVSDGNVAQMDWTFAEIVQRAADGVTLEPGEVI
                     GSGTVGTGCFLELNGSGITKDQWLRPGDVVSLAIDGLGTLAHTIVEDPAK"
     gene            284..796
                     /locus_tag="P5164_18570"
     CDS             284..796
                     /locus_tag="P5164_18570"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRY45942.1"
                     /translation="MKDDLLALLDYNLWANRLLARSLAPLSHEDYVREMGGGWPSLRA
                     TLVHLAGATRAWAERFDGRDATSLPTVEELPNLADALRTLDEADARVAAFLGTLGPER
                     LAAPLVWKNLKGEPKAAPLWAVLRHVVNHASYHRGQAAAMLRRLGGTPAPTDLVRWAI
                     ERHEAGAFEA"
     gene            802..1500
                     /locus_tag="P5164_18575"
     CDS             802..1500
                     /locus_tag="P5164_18575"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRY45943.1"
                     /translation="MIETSFERLVRRAMILGIGNLVTPVYFRWLNEVRAEGDGLLKEL
                     PRQNVVFLSNHQTYFTEAIAFYDLLYIVHGLPYEDPHLRFSAATETMKKNLLTKVLTY
                     AGGVTFKRSFREAGKEVNRPVDLEGVSKIEEAIATGWLLHFPTGTTQKNAPIRPGMAQ
                     LLHRTKPIAVPMRVDGFRDLLLHKQFPGRTGKKATIRLFPPMDLADFYAQPFDKERGR
                     KVIEELGKFILDPA"
     gene            complement(1481..1765)
                     /locus_tag="P5164_18580"
     CDS             complement(1481..1765)
                     /locus_tag="P5164_18580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45944.1"
                     /translation="MPVRKFRSIEDMKAARGYERTDPRLSRIIEGLWDFGRKTARLRF
                     PPGVYRYRNVEEMNARTAEWAEENFRAFRARRDAESEDAPAGPDSTPGRG"
     gene            complement(1765..2235)
                     /locus_tag="P5164_18585"
     CDS             complement(1765..2235)
                     /locus_tag="P5164_18585"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRY45945.1"
                     /translation="MNYAASRRILSAFEEEGVRYLVFGGAALNLHGLPRFTEDLDVFI
                     APTRENVEAVKRALRKVYDDPSIDEISADELVGEYPALQYVPPEGDFHVDILTRLGEA
                     FRFEDLEPDETTLDGVKVRLVSPRTLYLMKKDTVRPKDRLDADGLRAKYGFEEP"
     gene            complement(2240..2629)
                     /locus_tag="P5164_18590"
     CDS             complement(2240..2629)
                     /locus_tag="P5164_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45946.1"
                     /translation="MEIKGAGGTPGGVGTFLGGLAMIVVGGYLLLQQVQVHSGFWDWF
                     GPSTFGLTLLPLLVGIGILFFDGKSVAGWLLAGAGALIIVAGIVANLQIWFRPTSLYN
                     TLIMLGLLAGGVGLVARSLKARPEAKG"
     gene            complement(2660..>2850)
                     /locus_tag="P5164_18595"
     CDS             complement(2660..>2850)
                     /locus_tag="P5164_18595"
                     /inference="COORDINATES: protein motif:HMM:NF014456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRY45947.1"
                     /translation="HSKNTKELVRIAEESKRTVFVGTAADLRAEDFAGVRKVGVTAGA
                     STPDYDVDAVVERLRAMG"
BASE COUNT          451 a         1047 c          937 g          415 t
ORIGIN      
        1 tcgacgcgcg gatgaccgcg cacgtgaacg ggatccaggt ctccgacggg aacgtcgcgc
       61 agatggactg gacgttcgcc gagatcgtgc agcgcgccgc cgacggcgtg acgctcgagc
      121 cgggcgaagt gatcggctcc gggaccgtcg gcaccggctg cttcctcgag ctgaacggct
      181 cggggatcac gaaggaccag tggctccgcc ccggcgacgt cgtgagcctc gcgatcgacg
      241 gcctcgggac gctggcccac accatcgtcg aggaccccgc gaagtgaagg acgacctcct
      301 cgccctgctc gactacaacc tctgggcgaa ccggctcctc gcccgctcgc tcgccccgct
      361 ctcccacgag gactacgtcc gcgagatggg gggcgggtgg ccctccctgc gggccacgct
      421 cgtccacctc gccggagcca cgcgcgcctg ggccgagcgc ttcgacgggc gcgacgcgac
      481 ctcccttccg accgtcgagg agctgccgaa cctcgccgac gccctccgga cgctcgacga
      541 ggccgacgcc cgcgtcgcgg cgttcctcgg gacgctcggc ccggagcgcc tcgccgcccc
      601 gctcgtctgg aagaacctga agggggagcc gaaggccgcg cccctgtggg ccgtcctccg
      661 ccacgtcgtg aaccacgcct cctaccaccg cgggcaggcc gccgcgatgc tacggcggct
      721 cggcgggacc ccggccccga ccgacctcgt ccgctgggcc atcgaacgcc acgaggcggg
      781 cgccttcgag gcctgacagc cgtgatcgag acctccttcg agcgcctcgt ccggcgcgcg
      841 atgatcctcg gcatcggaaa cctcgtgacc ccggtctact tccgatggct gaacgaggtg
      901 agggcggaag gagacgggct cctgaaggag cttccgcgcc agaacgtcgt cttcctctcg
      961 aaccaccaga cctacttcac cgaggcgatc gcgttctacg accttctcta catcgtccac
     1021 ggcctcccgt acgaggaccc gcacctgcgc ttctcggcgg cgacggagac gatgaagaag
     1081 aacctcctga cgaaggtcct cacctacgcg ggaggcgtca cgttcaagcg ctccttccgg
     1141 gaggcgggca aggaggtcaa ccggcccgtc gacctggagg gggtctcgaa gatcgaggag
     1201 gcgatcgcga cggggtggct cctccacttc ccgaccggga cgacgcagaa gaacgcgccg
     1261 atccgccccg ggatggcgca gctcctccac cggacgaagc cgatcgccgt cccgatgcgg
     1321 gtcgacggct tccgcgacct cctcctgcac aagcagttcc ccgggagaac ggggaagaag
     1381 gccacgatcc gcctcttccc gccgatggac ctcgcggact tctacgcgca gccgttcgac
     1441 aaggagcggg gccggaaggt gatcgaggag ctcgggaagt tcatcctcga cccggcgtag
     1501 agtcgggccc cgcgggagcg tcctcgctct ccgcgtcccg acgggcacgg aaggcgcgga
     1561 agttctcttc cgcccactcc gccgtccgcg cgttcatctc ctcgacgttc cggtatcgat
     1621 agacgccggg cggaaaccga agcctggccg tcttccggcc gaagtcccag agcccctcga
     1681 tgatgcgaga gagccgggga tcggtccgct cgtagccgcg agccgctttc atatcctcga
     1741 tggaccggaa cttccggacg ggcatcacgg ctcctcgaac ccgtacttcg cccgaaggcc
     1801 gtcggcgtcg agccggtcct tcgggcggac cgtgtccttc ttcatgaggt agagcgtccg
     1861 cggcgagacg agcctcacct tcacgccgtc gagcgtcgtc tcgtccggct cgaggtcctc
     1921 gaaccgaaat gcctctccca ggcgcgtgag gatgtcgacg tggaagtccc cttcgggagg
     1981 cacgtactgc agcgccgggt actcgcccac gagctcgtcc gccgagatct cgtcgatgct
     2041 cgggtcgtcg tacaccttcc ggagcgcacg cttgaccgcc tcgacgttct cgcgggtcgg
     2101 cgcgatgaag acgtcgagat cctcggtgaa gcgcgggagt ccgtggaggt tcaatgcggc
     2161 ccctccgaaa acgaggtagc ggaccccctc ctcctcgaac gccgagagga tcctccggct
     2221 cgccgcgtag ttcacccgcc taccccttcg cctccggcct cgccttcagc gaccgcgcga
     2281 cgaggcccac gcctccggcg aggaggccga gcatgatcag ggtgttgtag aggctcgtcg
     2341 gccggaacca gatctgcagg ttcgcgacga tgccggcgac gatgatgagc gccccggccc
     2401 ccgccaggag ccagccggcg accgacttcc cgtcgaagaa gaggatcccg atcccgacga
     2461 ggagcgggag cagcgtgagg ccgaacgtgc tcggcccgaa ccagtcccag aagccgctgt
     2521 ggacctgcac ctgctggagg aggaggtagc ccccgacgac gatcatcgcg aggccgccga
     2581 ggaaggtccc gacgccgccc ggcgtcccgc ccgcgccctt gatctccatg cgcgggagtc
     2641 tacgccgggc gcgggccgtt cagcccatcg cccggagccg ctcgacgacc gcgtccacgt
     2701 cgtagtctgg agtggaggcg ccggcggtga cgccgacctt ccgcaccccc gcgaagtcct
     2761 ccgcgcggag gtcggcggca gtcccgacga agacggtccg cttcgactcc tccgcgatcc
     2821 ggacgagctc cttcgtgttc ttcgagtgac
//
LOCUS       DAOTEZ010000509         2832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_28055_length_2832_cov_4.418797, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000509 DAOTEZ010000000
VERSION     DAOTEZ010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28055_length_2832_cov_4.418797"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="P5164_18600"
     CDS             complement(<1..1028)
                     /locus_tag="P5164_18600"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRY45948.1"
                     /translation="MLERSARLGPYEILDPLGAGGMGEVYRARDGRLGREVAIKVLRG
                     AFARDGYRIARFEKEARALAALSHPNVLAIHDFGRAGDVVYAVTELLDGETLRDRLRR
                     DRPSWRKSAEIAAAAADGLAAAHARHLVHRDLKPENVFLTSDGRVKVLDFGLAKVVEP
                     ILPEASTLTSPASTAEGRLLGTVAYMSPEQARGLGVDSRSDVFSLGTVLYEMLSGRRP
                     FERATAADTISAILHEDPDPLDADAAIPPALAAIVARCLEKQPEERFHSAHDLAIALR
                     SLSAESHSGARPVRSRGRPARARPVLLAGAAVAVLASAALLVGDRSRGRPGRETSRVV
                     ARPVTSMPG"
     gene            1164..1754
                     /locus_tag="P5164_18605"
     CDS             1164..1754
                     /locus_tag="P5164_18605"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HRY45949.1"
                     /translation="MENPGGLAPLARALERCRAAPDESVPADRKAAVAAILRERSGEV
                     ELLFIRRAEHPRDPWSGQMGLPGGRVEPADASPLDAALRETREEIGLDLLAVGRPLGR
                     LSEVRTHLPLGSVPHSVVPFAFSVDGDPLLTPNEEVQEALWVPLAFLADGRNRSSFTW
                     VRKGLPLPMPCLRYEGRVIWGLTLRIVDELVLVAGA"
     gene            complement(1768..2550)
                     /locus_tag="P5164_18610"
     CDS             complement(1768..2550)
                     /locus_tag="P5164_18610"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRY45950.1"
                     /translation="MYFRLVRLKVRPESFCAFRDYYESRILPGLESAGGCLYAALLRP
                     VRPAEETETAETACDSLTLWESEERADAYVDSGRYDELLDGADPFLAGATDWSADLAR
                     LEVGARPPLPDPKVETGSVEALREADDGPRRPALFLRVVDHRVDPGGQAALRRTWEEE
                     VAPALLATPGCLAAYLVEGLHGRAQALSFTFWSDEESAVRYEMSGRFDDLARKLAPHL
                     SGLYRWRLALDPDGAVRDVRGSDLAVSPFRVVVGRKLGPPEG"
     gene            complement(2561..>2832)
                     /locus_tag="P5164_18615"
     CDS             complement(2561..>2832)
                     /locus_tag="P5164_18615"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HRY45951.1"
                     /translation="GLIPGAKYGEGELVMEPGDVLLVYTDGITEAADPDDEEFGEARL
                     LEAALRGRMLPLREMATVLERDLDAFARGVPFHDDRTLVLVRRSA"
BASE COUNT          377 a          989 c         1099 g          367 t
ORIGIN      
        1 ccgggcatcg aggtgacggg tcgcgcgacg acccgggagg tctcgcggcc gggtcgtccg
       61 cgagaccgat cgcccacgag cagcgcggcg gacgcgagga cggcgacggc ggcccccgcg
      121 agcagaacgg gacgggcgcg cgccgggcgc ccccgggagc gcaccggccg ggcgccggag
      181 tggctctcgg ccgagaggct gcggagggcg atcgccaggt cgtgtgccga gtggaagcgc
      241 tcctccggct gcttctcgag gcagcgagcg acgatggcag cgagcgccgg cgggatcgcc
      301 gcgtcggcgt cgaggggatc ggggtcctcg tgcaggatcg ccgagatcgt gtcggccgcc
      361 gtcgcgcgct cgaaggggcg ccggcccgag agcatctcgt agaggaccgt cccgagcgag
      421 aagacgtcgg aacgggagtc gacgccgagg ccgcgggcct gctccggcga catgtaggcc
      481 acggtcccga ggaggcgccc ctccgcggtg ctcgcgggcg acgtgagcgt ggacgcctcg
      541 gggaggatcg gctcgacgac cttcgcgagg ccgaagtcga ggaccttcac gcggccgtcc
      601 gaggtgagga agacgttctc gggcttcagg tcgcggtgga cgaggtgccg ggcatgggcg
      661 gcggcgagcc cgtctgcggc ggccgcggcg atctcggccg acttgcgcca ggacgggcga
      721 tcgcggcgga ggcggtcccg gagagtctcg ccgtcgagga gctcggtgac ggcgtagacg
      781 acgtcgccgg cgcggccgaa gtcgtggatc gcgaggacgt tcgggtgcga gagcgccgcg
      841 agcgcccggg cctccttctc gaaccgggcg atccggtagc cgtcgcgcgc gaaggcgccg
      901 cgcaggacct tgatcgcgac ctcgcgcccg agccgcccgt cgcgcgcccg gtaaacctcg
      961 cccatccccc cggccccgag cgggtcgagg atctcgtacg gaccgagcct cgcgctcctt
     1021 tcgagcatgt cgaagtccgt tcgttccggg ctgttccagg agaactgtgg tcttccggga
     1081 cggccgcgtc aacgggaatc ggggggacgc ccccgcccgg cccttcggcg cggatccgca
     1141 ggcaccgatg ccagaatcgg ggcgtggaga atccaggcgg tctcgccccc ctcgcgcggg
     1201 cgctcgaaag gtgccgcgcc gccccggacg agagcgtccc ggcggaccgg aaggcggccg
     1261 tcgccgcgat tctccgggag cgttcgggcg aggtggagct cctcttcatc cgccgggccg
     1321 agcacccgcg cgacccgtgg tcgggccaga tggggcttcc gggcggacgc gtcgagccgg
     1381 cggacgcctc cccgctcgac gcggccctcc gggagacgcg ggaggagatc ggcctcgacc
     1441 tcctcgcggt cgggcgtcct ctcgggcgcc tctccgaggt ccggacgcac cttccgctcg
     1501 gctccgtccc ccacagcgtc gtgccgttcg ccttctcggt cgacggagac ccgctcctca
     1561 cgccgaacga ggaggtccag gaggccctct gggtcccgct cgcgttcctc gcggacggaa
     1621 ggaaccggag ctcgttcacc tgggtgagga aggggctgcc gcttccgatg ccctgcctcc
     1681 gctacgaggg gcgggtcatc tggggtctca cgctccggat cgtcgacgag ctcgtcctcg
     1741 tcgcgggggc gtgaggcgcc gacgcgttca cccctccggc gggccgagct tccggcccac
     1801 gacgacccgg aatggggaga ccgcgaggtc gcttccgcga acgtcgcgga cggcgccgtc
     1861 cgggtcgagg gcgagccgcc agcggtagag gccggaaaga tggggagcga gctttcgcgc
     1921 gagatcgtcg aaccggccgc tcatctcgta tcggaccgcg ctctcctcgt cggaccagaa
     1981 ggtgaaggag agcgcctgcg cgcgtccgtg cagcccttcg acgaggtagg cggcgaggca
     2041 gccgggtgtc gccagaaggg cgggggcgac ctcctcctcc caggtccgcc ggagcgcggc
     2101 ctggccgccg gggtcgaccc tgtggtcgac gacgcgcagg aagagcgcgg gacgcctcgg
     2161 tccgtcgtcg gcctcccgaa gcgcctcgac cgagccggtc tcgaccttcg ggtcgggcag
     2221 gggaggcctg gcgcccacct cgagccgcgc gaggtcggcc gaccagtccg tcgcgccggc
     2281 gaggaagggg tcggcgccgt cgaggagctc gtcgtagcgg ccggaatcga cgtaggcgtc
     2341 ggccctctcc tcgctctccc agagcgtgag agagtcgcag gccgtctcgg ccgtctcggt
     2401 ctcctcggcc ggccggacgg ggcggaggag cgcggcgtag aggcatccgc cggccgactc
     2461 gaggccgggg aggatccggc tttcgtagta gtcgcggaac gcgcagaagc tctcgggccg
     2521 gaccttcagg cggacgaggc ggaagtacat ggccggctcc tcacgccgac cttcggacga
     2581 ggacgagagt ccggtcgtcg tggaacggga cgccgcgagc gaaggcgtcg aggtcgcgct
     2641 cgaggacggt cgccatctcg cgaagcggga gcatgcggcc ccggagggcc gcttcgagga
     2701 gccgcgcctc gccgaactcc tcgtcgtcgg gatcggcggc ctccgtgatc ccgtcggtgt
     2761 agacgaggag gacgtctccc ggctccatca cgagctcgcc ttccccgtac ttcgcccccg
     2821 ggatgagccc ga
//
LOCUS       DAOTEZ010000510         2818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_28265_length_2818_cov_6.777416, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000510 DAOTEZ010000000
VERSION     DAOTEZ010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28265_length_2818_cov_6.777416"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            239..649
                     /locus_tag="P5164_18620"
     CDS             239..649
                     /locus_tag="P5164_18620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRY45952.1"
                     /translation="MFARNAARTFALALGLVALLAGASSAAAEETAPAKKSVVNVNQA
                     SADELARLPRVGPSLAGKIVAHREQHGPFKRAQDLMEVKGIGEKMFAILQPYLVVSGP
                     TTLTEKVSSKGTTARKAPKPAKEKAPAITEKAGR"
     gene            726..1331
                     /locus_tag="P5164_18625"
     CDS             726..1331
                     /locus_tag="P5164_18625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45953.1"
                     /translation="MPHVRRPFRGITLPEVLTTLAMLSLAAGFSASAVHALAGQTSLR
                     AAAHEVASVFTQARGRAIHRSIQCGVKWIARDGDLTLEIHDDGDGDGVRSDDIESGTD
                     PLVYGPVSVRKRWPKVTVGFIPGFVARDPKGNPVGDLSDPVRFGRSDIASFSPLGDCS
                     PGSVWLGDTRSRQSLVRLSPTTATITIYEWAAARQGWIRVW"
     gene            complement(1356..2084)
                     /locus_tag="P5164_18630"
     CDS             complement(1356..2084)
                     /locus_tag="P5164_18630"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRY45954.1"
                     /translation="MRALVPAAGFGTRFRPATLSTPKPLLPLLGVPILVRLFRHLKAQ
                     GITSAVLNAHHLAETLMAAVGGSCEGIPVAFSPEDPILGTAGAIRRASERGLLGDGPF
                     LVVNGDLFTTLPFAPLVSALGAPGVVSALGVLPHAAPGETALWGDAEGRLVSLGNERP
                     FPEATGPWLFTGLQCAGPALVGRIPPGVSELARDVLRPSTERRDGAFALVPYRVPEDG
                     LWFDLGSPAKLAAAEEAIAAAGLA"
     gene            complement(2081..2647)
                     /locus_tag="P5164_18635"
     CDS             complement(2081..2647)
                     /locus_tag="P5164_18635"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HRY45955.1"
                     /translation="MSEGPREIEAKFRVADRPELEARLAALGAVPGAREDEVNLLLDD
                     DDASLRSRGCALRVRTVGGRGLLTYKGPASFDGGVKARLELESGVDAPESLLPLLDAL
                     GFRPRFRYDKRRTPWRFVDAARPLVVVDETPLGLFAEIEGEAAAVRTLAAELGVAEES
                     FLRASYQGLWQAAREAEPDLPEDMVFPE"
BASE COUNT          425 a         1018 c         1040 g          335 t
ORIGIN      
        1 tgcgcgaggc aagtcacttt gctgacgcga tttgagtcga gcgaacgccg tacggcgggt
       61 ggtttgcgac gtccgggcca gaccacgaga cacccgagac gagaggagga gagggatgac
      121 gaaggaagcc tgaccggccg cgatcgcccg cacgaagaag agacgaaacg aacgaagccc
      181 cgatcgaaac gccccgaacc ggggcagatc ccccgaaaga gaagagaagg agaacacgat
      241 gttcgctcgc aacgctgccc gcacgttcgc cctcgccctc ggcctcgtcg ccctcctcgc
      301 cggcgcctcc tccgcagcgg cggaagagac cgctcccgcg aagaagagcg tcgtcaacgt
      361 gaaccaggcc tccgccgacg agctcgcgcg tctgccgcgc gtcggcccgt cgctggccgg
      421 gaagatcgtc gcccaccggg agcagcacgg cccgttcaag cgggcgcagg acctgatgga
      481 ggtgaagggg atcggcgaga agatgttcgc gatccttcag ccgtacctcg tcgtctccgg
      541 ccccacgacg ctgacggaga aggtctcctc gaagggaacg accgcccgga aggcgccgaa
      601 gcccgcgaag gagaaggctc ctgccatcac ggagaaggcc ggccgctgag gcgccgcgcg
      661 ggccgggggc tcgccccccg gcccgcgccc gtcttcgaga cgacccgaga tctcgaggtg
      721 ccgccgtgcc gcacgtccgc cgccccttcc gcgggatcac cctccccgag gtcctgacga
      781 ccctcgcgat gctttcgctc gccgccggct tctcggcatc cgccgtccac gcgctcgccg
      841 gccagacctc gctgcgcgcc gcggcccacg aggtcgcgtc cgtcttcacg caggcacgcg
      901 gccgcgcgat ccaccggagc atccagtgcg gcgtgaagtg gatcgcccgc gacggcgacc
      961 tgacgctcga gatccacgac gacggggacg gagacggagt gaggagcgac gacatcgaga
     1021 gcgggaccga cccgctcgtc tacggcccgg tctccgtccg caagcgctgg ccgaaggtga
     1081 cggtcggctt catccccggc ttcgtcgcgc gcgacccgaa ggggaacccc gtgggcgacc
     1141 tctccgaccc ggtccgtttc ggccgctccg acatcgcgag cttctcgccg ctcggcgact
     1201 gctctcccgg ctcggtctgg ctgggtgaca cgaggagccg gcagtcgctc gtccgccttt
     1261 ccccgacgac cgcgacgatc acgatctacg agtgggccgc cgcgcggcag gggtggattc
     1321 gcgtctggtg atggctcttg ggggtacccg ccggctcagg cgaggcccgc cgcggcgatc
     1381 gcctcctcgg ccgcggcgag cttcgccggg gagccgaggt cgaaccagag gccgtcctcc
     1441 gggacccggt acgggacgag cgcgaaggcg ccgtcccgtc gttccgtcga ggggcggagg
     1501 acgtcgcgcg cgagctcgga gacgccgggc gggatccgac cgacgagcgc gggaccggcg
     1561 cattgcaggc ccgtgaagag ccacgggccg gtcgcctccg ggaacggacg ctcgttgccg
     1621 agggagacga gccgcccctc ggcgtcgccc cagagcgccg tctcgcccgg cgcggcgtgc
     1681 gggaggacgc cgagcgccga gacgacgccg ggcgcgccga gcgccgagac gagcggcgcg
     1741 aaggggagcg tcgtgaagag gtcgccgttg acgacgagga acggcccgtc gccgagaagg
     1801 ccgcgctcgg acgcgcggcg aatcgcgcct gccgtcccga ggatcgggtc ctccggcgag
     1861 aaggcgacgg gaattccctc gcaggaaccg ccgaccgccg ccatgagcgt ctcggcgagg
     1921 tggtgcgcgt tgaggaccgc cgaggtgatg ccctgcgcct tcaggtgccg gaagagccgg
     1981 acgaggatcg ggacgccgag gagcgggagg agcggcttcg gggtggagag cgtggcgggg
     2041 cggaagcgcg tgccgaagcc cgcggccggg acgagcgctc tcattccggg aagaccatgt
     2101 cctcggggag gtccggctcg gcctcgcggg ccgcctgcca caggccctgg tacgacgcgc
     2161 gcaggaacga ctcctccgcg acgccgagct ccgcggcgag ggtccggacg gccgcggcct
     2221 ccccctcgat ctcggcgaag aggccgagcg gcgtctcgtc gacgacgacg agggggcgcg
     2281 cggcgtcgac gaagcgccac ggcgtccgcc gcttgtcgta ccggaagcgg gggcggaagc
     2341 cgagggcgtc gagaagcggg aggaggcttt ccggcgcgtc gacgccgctc tccagctcga
     2401 gtcgcgcctt gacgcccccg tcgaaggagg ccggtccctt gtaggtcagc aggccgcggc
     2461 cgccgaccgt ccggacgcgc agcgcgcagc cacgcgagcg aagagaggcg tcgtcgtcgt
     2521 cgaggaggag gttcacctcg tcctcgcggg ccccggggac ggcgccgagc gcggcaaggc
     2581 gcgcctcgag ctccggcctg tcggcgaccc ggaacttcgc ctcgatctcg cgggggccct
     2641 cgctcacctc cctagaatag cggccgtgcc gctctaccgt ctctcgggca tccggctccg
     2701 cgccccgcac gcgccggacg aggcgctcct cgcccgtctc ctcggcgtcc cgcccgggga
     2761 cgtctcggag gtggcggtcg tccgccgctc gctcgacgcg cggaagaagc ccgacctc
//
LOCUS       DAOTEZ010000511         2816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_28302_length_2816_cov_5.559580, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000511 DAOTEZ010000000
VERSION     DAOTEZ010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28302_length_2816_cov_5.559580"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1201
                     /locus_tag="P5164_18640"
     CDS             113..1201
                     /locus_tag="P5164_18640"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HRY45956.1"
                     /translation="MAAPRIVLHEVGPRDGLQVEKTVVPTETKLRWISALCEAGLDVV
                     QVGSFVHPEKVPQMADTDALFRALTAPGAERKGHALYSGLVLNEKGLDRALACGVEMV
                     CLGVSASETHSRKNTGMGTAEATERIVATARKAVEAGRKVQVSVQSAFGCGFEGNVPE
                     ERVIDIARAYVDAGLLTIALADTAGHAYPEQVERLYGRLLALHPGIEAACHLHDTYGA
                     GMANVYAALRAGAVSLESSFAGLGGCPFTKVAAGNVATEDLVHTLQRDGKRTDVDLEG
                     LIAVAKDVEAFFGREMPGRVHRTGPVPQLRREARAEGFGEPGVRVGSAFASQPGCSAV
                     LREHNEPTDGAPALSAIDGRPQPGGRLI"
     gene            1213..2412
                     /locus_tag="P5164_18645"
     CDS             1213..2412
                     /locus_tag="P5164_18645"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRY45957.1"
                     /translation="MTKKALDGVVVLDLTNVLSGPFATLHLALLGAEVIKVENPTDGD
                     LARKLGNVPKLNKELMGTSFLAQNANKKSLTLNLKKPEAKEIFKKLAATADVVVENFR
                     PGVMTRLGVGWEVLREINPRLVYCAISGFGQDGPDADKPAYDQIIQGLSGEMDVNGDE
                     RLHPLRAGFPVCDTVGGMNAAFAILAALYHRQRTGEGQAIDVALLDSIMPLMGWVAAN
                     LLIGGAPPVAIGNDNFTAAPSGMFKTKDGHINIAANKQEQWESVCDVLGVPELKADPR
                     FAERDTRKKNRHALTPLLEAKLAERPTSEWVDLLNAKDVPSGDILTLEAALKQPQVVH
                     RKTLESVETPGVGTLPLFNLTAKLEKTPGSIDAPPPRLGAHTAEILGRIGYAAEDVAR
                     LKAEGVV"
     gene            2421..>2816
                     /locus_tag="P5164_18650"
     CDS             2421..>2816
                     /locus_tag="P5164_18650"
                     /inference="COORDINATES: protein motif:HMM:NF012550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HRY45958.1"
                     /translation="MGKTVVEKILARAAGLPEVTAGDVVEPRVDVAMSHENAALVINQ
                     FLEIYEGTGVTPHPWDVSKIAIIFDHRVPAESPKTASNQKKIREFVDAHGIARFHDVR
                     GDVGGICHQILPEYGYVRPGQVVVGTDSHT"
BASE COUNT          470 a         1021 c          961 g          364 t
ORIGIN      
        1 ctcgaggcgg ccgtggcgaa ggtcatcgtc gaggggaagg tccgcacgta cgacatgggc
       61 ggctcggcga cgaccctcga gatgggcgag gccgttgccc gggcgctctg acgtggcggc
      121 gccccgcatc gttctccacg aggtcggccc cagggacggc ctccaggtcg agaagacggt
      181 cgtcccgacg gagacgaagc tccgctggat ctcggccctc tgcgaagcgg gcctcgacgt
      241 cgtccaggtc ggctccttcg tccaccccga gaaggtgccg cagatggccg acaccgacgc
      301 gctcttccgc gcgctgacgg cgccgggcgc ggagcggaag gggcacgcgc tctactcggg
      361 cctcgtcctg aacgagaagg ggctcgaccg cgcgctcgcc tgcggcgtcg agatggtctg
      421 cctcggcgtc tcggcctccg agacccacag ccggaagaac accggcatgg ggacggccga
      481 ggcgacggag cggatcgtcg cgacggcgag gaaggccgtc gaagcgggcc ggaaggtcca
      541 ggtctccgtc cagtcggcct tcggctgcgg cttcgagggg aacgtcccgg aggagagggt
      601 gatcgacatc gcccgtgcct atgtcgacgc cggcctcctg acgatcgccc tcgccgacac
      661 ggccgggcat gcgtaccccg agcaggtcga gcggctctac gggaggctcc tcgcgctcca
      721 tcccgggatc gaggcggcct gccacctcca cgacacctac ggcgccggca tggcgaacgt
      781 gtacgccgcg ctgcgcgcgg gcgccgtctc cctcgagtcc tccttcgccg gcctcggcgg
      841 ctgccccttc acgaaggtcg ccgccgggaa cgtcgcgacc gaggacctcg tccacaccct
      901 ccagcgagac gggaagcgga ccgatgtcga cctcgaaggc ctgatcgccg tcgcgaagga
      961 cgtcgaggcg ttcttcggac gcgagatgcc cggccgcgtc caccgcaccg gccccgtccc
     1021 ccagctgaga agagaagccc gcgcggaggg gttcggcgaa cccggcgtcc gggttggctc
     1081 cgccttcgcc tcgcagccag gctgctcggc ggtcctccga gaacataacg agccgaccga
     1141 tggtgccccg gcgctctccg cgatcgacgg tcggccacaa cccggtggac gactcatatg
     1201 aaagaccccg gcatgacgaa gaaggcgctc gacggcgtcg tcgtcctcga cctgacgaac
     1261 gtcctctccg ggccgttcgc gacgctccac ctcgccctcc tcggcgccga ggtgatcaag
     1321 gtcgagaacc cgacggacgg cgacctcgcg cgcaagctcg gcaacgtccc gaagctcaac
     1381 aaggagctga tggggacgag cttcctcgcg cagaacgcga acaagaagtc gctcaccctg
     1441 aacctcaaga agcccgaggc gaaggagatc ttcaagaagc tcgcggcgac cgccgacgtc
     1501 gtcgtcgaga acttccgccc cggcgtcatg acgcggctcg gcgtcggctg ggaggtcctc
     1561 cgcgagatca acccgcgcct cgtctactgc gcgatctccg gcttcggcca ggacggcccc
     1621 gacgccgaca agcccgccta cgaccagatc atccaggggc tttcgggcga gatggacgtg
     1681 aacggcgacg agcggctcca cccgctccgg gccggcttcc cggtctgcga cacggtcggc
     1741 gggatgaacg ccgcgttcgc gatcctggcg gcgctctacc accgccagcg gacgggcgag
     1801 gggcaggcga tcgacgtcgc gctcctcgac tcgatcatgc cgctcatggg gtgggtcgcc
     1861 gcgaacctcc tcatcggggg cgccccgccc gtcgcgatcg gcaacgacaa cttcaccgcc
     1921 gccccgtcgg gaatgttcaa gacgaaggac gggcacatca acatcgccgc gaacaagcag
     1981 gagcagtggg agtcggtctg cgacgtcctc ggcgtcccgg agctgaaggc cgacccgcgc
     2041 ttcgccgagc gcgacacgcg gaagaagaac cgccacgcgc tgacgccgct cctcgaggcg
     2101 aagctggccg agcgccccac gtccgagtgg gtcgacctcc tgaacgcgaa ggacgtcccc
     2161 tcgggcgaca tcctgacgct cgaagccgcg ctgaagcagc cgcaggtcgt ccaccggaag
     2221 accctggaga gcgtcgagac cccgggcgtc gggacgctcc ccctcttcaa cctgacggcg
     2281 aagctcgaga agacccccgg gtcgatcgac gcgccgccgc cgcgcctcgg cgcgcacact
     2341 gcagagatcc tgggccggat cggctacgcg gcggaggacg tcgcgcggct gaaggccgag
     2401 ggagtcgtct aggaggcccc atgggcaaga ccgtcgtcga gaagatcctg gcgcgcgccg
     2461 cgggcctccc cgaggtgacc gccggggacg tcgtcgagcc gcgcgtcgac gtggcgatga
     2521 gccacgagaa cgccgcgctc gtcatcaacc agttcctcga gatctacgag gggacgggcg
     2581 tgaccccgca cccctgggac gtctcgaaga tcgcgatcat cttcgaccac cgcgtcccgg
     2641 ccgagtcgcc gaagacggcg tcgaaccaga agaagatccg cgagttcgtc gacgcccacg
     2701 gcatcgcccg cttccacgac gtgcggggcg acgtcggcgg catctgccac cagatcctcc
     2761 ccgagtacgg ctacgtgcgg cccgggcagg tcgtcgtcgg caccgactcg cacacg
//
LOCUS       DAOTEZ010000512         2810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_28385_length_2810_cov_5.184755, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000512 DAOTEZ010000000
VERSION     DAOTEZ010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28385_length_2810_cov_5.184755"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="P5164_18655"
     CDS             <1..446
                     /locus_tag="P5164_18655"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRY45959.1"
                     /translation="TGKLLALDEAKGQAEVEVGGKRMKVGTADLRPATGAVASTPASK
                     WAPIPRASVGEAKVALATRAAAPRGEIVLVGLRVDAALPEVERAINDALLSGKGSLRI
                     VHGFGSGRLAAAVREFLADHPGVSSHRPGDDTEGGDAATIAELRD"
     gene            570..2366
                     /gene="dnaG"
                     /locus_tag="P5164_18660"
     CDS             570..2366
                     /gene="dnaG"
                     /locus_tag="P5164_18660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRY45960.1"
                     /translation="MDASYEITDDLKHDVLAATDIVALIGAVTGLKKAGNSWKGLCPF
                     HGEKTPSFHVHPDRGFYYCFGCGAKGDAITFVRETEKLEFHEAVAYLARRAGIPLPVR
                     RTGTRADRAKESRAAEALVAAARFFREQLPKSRAAVAVLEGRGVPREEWDPLGFGAAP
                     DSWDGLLRALSGSFTEEVLLEAGLLQKNADSGRVYDRFRNRLTLEIRDGRGEVLAFGG
                     RALGDDPAKYINSPETSRFTKGRVLYGLDRAREGARKSERLVLCEGYFDRIAFERAGV
                     PWAVASMGTALTTTQADLLARHVPSVVVAYDGDAAGLAAAWKAFPLLVERGVRVFHVA
                     FPDGHDPDSFLRARGPEALRTSVESARPILDVLAASIPPASGDAAERAARINEAKAIL
                     SVAPDRVLRHELLAAFSRASGVPLDLLHDRSRRTPLRPEAAASPSVARPVPEAEERVL
                     ESLLSTWPAGAEIVPRIPLELFSHPDVREVAEKLKSLAPKADALDFSELQSHLGGGAG
                     ALVARLLLSDSRQTDEIPAAPDLEKLRKPLLQLKIRRLEERGVELQPLIAQAEARHDR
                     DGRTALLAEKQKLSEEVRRMKQELRRPDEGRG"
     gene            2378..>2810
                     /locus_tag="P5164_18665"
     CDS             2378..>2810
                     /locus_tag="P5164_18665"
                     /inference="COORDINATES: protein motif:HMM:NF015912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HRY45961.1"
                     /translation="MTVEEKHPELRGLIALGKERGYLLYDEIFQALPEEISNTPDELD
                     DVYIRLGDFGIEIVDVPEKATPKKDGAAQTEAVLPDLTTVEKTNDPVRMYLREMGTVK
                     LLDREGEVEIARRIERGEARIYQALSTNRIVLEEILRIVEAA"
BASE COUNT          427 a         1016 c          984 g          383 t
ORIGIN      
        1 cgacggggaa gctcctcgcg ctcgacgagg cgaaggggca ggccgaggtc gaggtgggcg
       61 ggaagcggat gaaggtcggc acggccgacc ttcggccggc caccggcgcc gtggcgtcga
      121 cgcccgcctc gaagtgggct cccatcccgc gcgcttccgt cggggaggcg aaggtcgcgc
      181 tcgccaccag ggccgccgct ccccgcgggg agatcgtcct cgtaggcctc cgcgtcgacg
      241 cggccctgcc ggaggtggag cgcgcgatca acgatgccct cctctccggc aaggggagcc
      301 tgcggatcgt ccacggcttc ggctccggcc gcctcgccgc agccgtccgg gagttcctcg
      361 ccgaccaccc cggcgtctcc tcccaccgcc ccggcgacga cacggaaggt ggcgacgcgg
      421 cgaccatcgc cgaattgagg gactgatgag cgcggactgg gggtgggggg tccggggggc
      481 gcgcgcagct caggcccccc ggcgagcgcc gagatgaggg actagaggaa tgaaggcggg
      541 gcccgatccg ttccgggttt gagagccgcg tggacgcttc ctacgagatc accgacgacc
      601 tgaagcacga cgtcctcgcc gcgacggaca tcgtcgccct catcggcgcc gtcaccggcc
      661 tgaagaaggc cggcaactcc tggaaggggc tctgcccgtt ccacggcgag aagaccccct
      721 cgttccacgt ccatcccgat cgcggcttct actactgctt cggatgcggg gcgaaggggg
      781 acgcgatcac cttcgtccgt gagaccgaga agctcgaatt ccacgaggcg gtggcctatc
      841 tcgcccgtcg cgccggcatc ccgctcccgg tccgccgcac ggggacgcgc gccgaccggg
      901 cgaaggagag ccgcgcggcc gaggcgctcg tcgccgccgc gcgcttcttc cgtgagcagc
      961 tcccgaagag ccgtgcggcg gtcgccgtcc tcgaggggcg cggcgtaccg cgagaggagt
     1021 gggacccgct cgggttcggg gcggccccgg actcctggga cgggctcctg cgcgctctct
     1081 cggggagctt caccgaagag gtcctcctcg aggccggact cctccagaag aacgccgact
     1141 cgggtcgcgt ctacgaccgc ttccgcaacc gcctgacgct cgagatccgg gacggccgcg
     1201 gggaagtgct cgcgttcggg ggccgcgcgc tcggcgacga cccggccaag tacatcaaca
     1261 gccccgagac gtcacgcttc acgaaagggc gcgtcctcta cggcctcgac cgggcgcgcg
     1321 agggggcgcg aaagagcgag cggctcgtcc tctgcgaggg ctacttcgac cggatcgcct
     1381 tcgagagggc cggcgtcccg tgggccgtcg cctcgatggg gaccgccctc acgacgaccc
     1441 aggccgacct gctggcccgg cacgttccct ccgtcgtcgt cgcctacgac ggcgatgccg
     1501 ccggcctcgc cgcggcctgg aaggccttcc ccctcctcgt cgagcggggc gtccgcgtct
     1561 ttcacgtggc cttccccgac gggcacgacc ccgactcgtt cctgcgcgcc cgcgggccgg
     1621 aggcgctccg gacgtcggtc gagtccgcgc ggccgatcct cgacgtcctc gccgcgtcga
     1681 tcccgcccgc ctccggcgac gcggcggagc gggcggcacg aatcaacgag gcgaaggcga
     1741 tcctctccgt cgcccccgac cgggtcctcc ggcacgagct cctcgcggcc ttctcgcgtg
     1801 cgagcggcgt accgctcgac ctccttcacg accggagtcg ccggaccccg ctccgccccg
     1861 aggccgcggc ctccccttcc gtcgcccgac cggtgccgga ggccgaggag cgcgtcctcg
     1921 aatccctcct ctcgacctgg ccggccgggg cggagatcgt cccccgcatt cccctggagc
     1981 ttttctcgca ccccgacgtg cgggaggtcg ccgagaagct caagtccctg gctccgaaag
     2041 cggatgccct tgatttttcc gaacttcaat cccatcttgg gggcggcgcg ggggcgctgg
     2101 tggcccgact cctcctatcg gactcccggc aaacggacga gatccccgca gcccccgacc
     2161 tggagaagct acgcaagcca ctcctccagc tcaagataag gcgactcgag gagcgagggg
     2221 tcgaactgca gccgttgatc gcacaggccg aggcccggca tgaccgggac ggccggactg
     2281 cccttctcgc cgagaagcag aagctttccg aggaggtgcg tcgtatgaag caggagctac
     2341 gccgcccgga cgaaggtcgg ggctgattgg ggaagacgtg acggtcgaag aaaagcaccc
     2401 ggagctgcgc gggctgatcg ccctcgggaa ggagcgggga tacctgctct acgacgagat
     2461 cttccaggcg cttcccgagg agatctcgaa cacccccgac gagctggacg acgtctacat
     2521 acggctcggg gacttcggga tcgagatcgt ggacgttccc gagaaggcga cgccgaagaa
     2581 ggacggggcc gcgcagacgg aggcggtcct tcccgacctg acgaccgtcg agaagacgaa
     2641 cgaccccgtc cggatgtacc tgcgcgagat ggggaccgtg aagctgctcg accgtgaggg
     2701 cgaggtcgag atcgcccggc ggatcgagcg gggcgaggcc cggatctacc aggcgctctc
     2761 cacgaaccgg atcgtcctcg aggagatcct ccggatcgtc gaggccgcca
//
LOCUS       DAOTEZ010000513         2808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_28415_length_2808_cov_5.628769, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000513 DAOTEZ010000000
VERSION     DAOTEZ010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28415_length_2808_cov_5.628769"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="P5164_18670"
     CDS             <1..265
                     /locus_tag="P5164_18670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45962.1"
                     /translation="GGAVVCAARTGRPLRPVGAAARRAPVLKRSWSRIWLPLPFSRVV
                     VVVGEPLPVPTGVGRTEWERLAGELTARLAAVRSRALEEVAGA"
     gene            262..1038
                     /locus_tag="P5164_18675"
     CDS             262..1038
                     /locus_tag="P5164_18675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45963.1"
                     /translation="MRRFLGYWLLLSPAYLVAAALSSWLLLNLVDVTYEAFVAWLLVP
                     VAQALALAATSRRAPLLPAGRPPLLALALLASVLAVVAAGFGRPFDARWGFLGPGQLQ
                     GNVPRILAILAGAAFVVTCARDRKGRPAVASLGALLLVLGLDAVQPFLASLPGALLPG
                     RGLFLGGLVTWGVLVALVFAAALAAQRALEETSAWAARLLGASLGVLFGALHGALLQL
                     FLHPWLEKPWSLLVPAAVSVAAALAAAAGLAAFVDRRESA"
     gene            1035..2036
                     /locus_tag="P5164_18680"
     CDS             1035..2036
                     /locus_tag="P5164_18680"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45964.1"
                     /translation="MTKGAPLRALVLARDLLLTLVALPFLVPLRVLPWNAAGRLGSLY
                     GLAAWVFLGTSRRAGLVNLRRAFGPSMSRAEAGRAVRAVFASLGRSLAEGIRFSALPG
                     RADGSWRALYDAEDPGLEARILADPRPKVFVTGHLGSWEVAMQMLDLALEGRGAAVAR
                     RVDNPFLDALLRRVRRGDGALIEKRGAVVPALAALRSGTSVAVLLDENGGRSGPFPLF
                     FGRPASTRKTAALLSARTGAPIVVGAAVRRGERFLFRLAFFEPPGDGVPTPSDVLETT
                     EKIVRVWEAWVREDPLQWRWIHWRWKTRPGGHEETYTRRDVNAAFAAQGPAAVEVSA"
     gene            2033..2788
                     /locus_tag="P5164_18685"
     CDS             2033..2788
                     /locus_tag="P5164_18685"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY45965.1"
                     /translation="MSTPFPDLRGRVAVVSGGTRGLGRTISLALAASGARLVLGYRAD
                     AAAAEETARACQDLGAEVRTVAANLVHPEEARALVAAAGDRVDVLVHSAALGSFKPLL
                     DVKPAQWDLTLGVNARAFLLLAREASERMPDGGRLVALSSLGGSRFIPDYGAIGPSKA
                     ALESVVRTLAVELGPRGILVNAVSAGLVPTTSVGLHPRFEELAAHARTASPLGRLGTP
                     EDVASAVLLLVSPLAGWITGQTLVVDGGASLTT"
BASE COUNT          260 a         1127 c          976 g          445 t
ORIGIN      
        1 gggcggagcc gtcgtctgcg cggcgcggac gggtcgcccc ctgcgccccg tgggagcggc
       61 ggcccggcga gcccccgtcc tgaagcgaag ctggtcgaga atctggctcc cgctcccctt
      121 ctcgcgcgtc gtcgtcgtcg tcggcgagcc tctgcccgtg ccgacagggg tgggccgcac
      181 ggagtgggaa cgcctcgccg gggagctcac cgcgcgtctc gcggcggtac ggagccgtgc
      241 cctggaggag gtggccgggg catgaggcgg ttcctggggt actggctcct cctctccccc
      301 gcgtacctcg tcgccgcggc cctctcgtcg tggctcctcc tgaacctcgt cgacgtgacg
      361 tacgaagcct tcgtggcgtg gctcctcgtc cccgtcgcgc aggccctcgc cctcgcggcg
      421 acctcccggc gcgcgcccct cctcccggcc ggccgccctc cgctcctcgc gctcgcgctt
      481 ctcgcgtctg ttctcgccgt cgtcgccgcg gggttcgggc ggccgttcga cgcgaggtgg
      541 ggcttcctcg gtcccggaca gctccagggg aacgtcccgc gcatcctcgc gatcctcgcc
      601 ggggccgcgt tcgtcgtgac ctgcgcccgc gaccggaagg ggcgccccgc cgtcgcgtcg
      661 ctcggcgcgc tcctgctcgt cctcgggctc gacgccgtgc agccgttcct cgcgtcgctc
      721 cccggcgctc tcctcccggg ccgagggctc ttccttggcg gcctcgtcac ctggggagtc
      781 ctcgtcgccc tcgtcttcgc cgcggcgctc gcggcgcagc gggcgctcga ggagacgagc
      841 gcctgggcgg cccgcctgct gggcgcctcg ctcggcgtgc tgttcggcgc ccttcacggt
      901 gcgctcctcc agctcttcct ccacccctgg ctcgagaagc cctggtcgct cctcgtcccc
      961 gccgccgtct ccgtcgccgc cgcgcttgcg gcggccgccg gcctggcggc cttcgtcgac
     1021 cggagggaga gcgcatgacg aagggcgctc ccctccgggc cctcgtcctg gcgcgcgatc
     1081 tcctcctcac gctcgtggcc ctgccgttcc tcgtgcccct gcgcgtcctt ccgtggaacg
     1141 ccgcgggccg cctcgggagc ctctacggcc tggcggcctg ggtcttcctg gggacgtcgc
     1201 ggcgggccgg cctcgtcaac ctccggcgcg ccttcggacc gtcgatgtcg cgcgccgagg
     1261 cgggccgcgc cgtccgagcc gtcttcgcga gcctcgggcg gagcctcgcg gaggggatcc
     1321 gcttctcggc cctgcccggc cgggccgatg ggtcctggag ggctctctac gacgccgagg
     1381 atcccgggct cgaggcgcgg atcctcgccg acccgcgtcc gaaggtcttc gtcacggggc
     1441 acctcggctc ctgggaggtc gcgatgcaga tgctcgacct ggcgctcgaa gggcgcggcg
     1501 cggccgtggc ccggcgggtc gacaacccct tcctcgacgc ccttctccgc cgcgtgcggc
     1561 gcggcgatgg cgccctcatc gaaaagagag gcgcagtcgt gcccgctctc gctgcgctcc
     1621 gctcgggcac gagcgtggcg gtcctcctcg acgagaacgg cggccgttcc ggtccgttcc
     1681 ctctattctt cgggcgcccc gcctcgaccc gcaagaccgc ggcgctcctc tccgcgcgca
     1741 ccggtgcgcc gatcgtcgtc ggcgccgccg tccgtcgcgg ggagcggttc ctcttccgcc
     1801 tcgcattctt cgagcctccc ggagacggcg tcccgacccc gagcgacgtc ctcgaaacga
     1861 cggagaagat cgtccgggtc tgggaagcct gggttcggga ggaccccctg cagtggcgtt
     1921 ggattcactg gcgctggaag acccgccccg gcgggcacga ggagacctac actcggcgcg
     1981 acgtgaacgc ggccttcgcg gcccagggcc cggccgccgt ggaggtctcc gcgtgagcac
     2041 gccctttccc gacctgcgcg gccgtgtcgc cgtcgtctcc ggcgggaccc gcggcctcgg
     2101 tcgcacgatc tctctcgcgc tcgccgcttc cggggctcgc ctcgtcctcg gctatcgcgc
     2161 cgacgccgcc gcggcggagg agaccgcccg cgcctgccag gacctcggcg ccgaggtccg
     2221 gaccgtcgcg gcgaacctcg tccaccccga ggaggcgagg gcgctcgtcg cggcggccgg
     2281 cgaccgggtc gacgttctcg tccattccgc cgccctcggc tccttcaagc cgctcctcga
     2341 cgtgaagccc gcgcagtggg acctgacgct cggcgtgaac gcccgggcgt tcctcctcct
     2401 cgcgcgcgag gcgtcggaac ggatgccgga cggagggagg ctcgtcgccc tctcgagcct
     2461 cggcggctcg cgcttcatcc cggactacgg cgcgatcggt ccgtcgaaag ccgccctcga
     2521 gtccgtcgtc cgcactctcg ccgtagagct gggtccgcgc ggcatcctcg tcaacgcggt
     2581 ctcggcgggg ctcgtgccga cgacgtcggt gggtctccac ccgcggttcg aggagctcgc
     2641 cgcccacgcg cgcaccgcat ctcccctcgg ccgcctcgga acgccggaag acgtggcctc
     2701 ggccgtcctc ctcctcgtct cgccgctcgc cggatggatc acggggcaga cgctcgtcgt
     2761 ggacggaggc gccagcctca cgacctgaac ggacacgacc gggcgaac
//
LOCUS       DAOTEZ010000514         2792 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_28655_length_2792_cov_7.323712, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000514 DAOTEZ010000000
VERSION     DAOTEZ010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28655_length_2792_cov_7.323712"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="P5164_18690"
     CDS             complement(<1..280)
                     /locus_tag="P5164_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRY45966.1"
                     /translation="MRTRSGRMRAFGAAVAVLSFALPAAAAPKGAGWQVPTSVRKLPN
                     GLTVVVSEDRSSPTFGLCVVYRIGFRLEPKGRTGFAHLFEHMMFQGTPN"
     gene            727..1848
                     /gene="metK"
                     /locus_tag="P5164_18695"
     CDS             727..1848
                     /gene="metK"
                     /locus_tag="P5164_18695"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRY45967.1"
                     /translation="MTSETDFLLTSESVTEGHPDKVCDLIADAVLDANIGPDENARVA
                     CEVLCKKDKVVLAGEITSKAGLKKADYEELVRGVIEGIGYVDPAEAFNAKGVKVLNFL
                     TRQSKEIGGAVKKATDAKKKDQGAGDQGIMFGYATKETDELLPLPIVLAHRLTRGLAE
                     ARRDFVPWLRPDGKSQVTVRYEGGLPKQVESVLVSTQHSPDVTREQIAAWVEKDLAPK
                     ALGEWMRDGIRFIANPSGSFVLGGPTADAGVTGRKIIVDSYGGAARHGGGAWSGKDPS
                     KVDRSGAYFCRWAARQVVKAGLARRAEIQVSYAIGEAKPMSVTVETFGTGDAKAAAAL
                     VSSFDFRPAAISERLGLRKPIYRKTTNYGHFKPGLPWEE"
     gene            1969..>2792
                     /locus_tag="P5164_18700"
     CDS             1969..>2792
                     /locus_tag="P5164_18700"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HRY45968.1"
                     /translation="MAVKDRPLFRRILEFDDLLRRRRVVNATNLATRWKTSTKTVQRF
                     VAFMRDEMDAPIVFDRKSGSFRYSDPAWHLPWIPVEGPGLFAIGVAAKVLQLYEGTPV
                     AEGLREAFERLSDVMPPEIRVSPGAFVERLYIHPQPIRLVDPAVWNAVATAIREKTVL
                     AVRYQKPSGETSARRLAPYGLVLAAGDWLVAAQDRDDDPGVVKTFYVSRIRGAETTPE
                     PFSVPRDFDLKRHFGETIGIFVGKEPFRFRVRFTKAMAAWVAEVRWHPKQKLTTLPS"
BASE COUNT          451 a          992 c          978 g          371 t
ORIGIN      
        1 cgttcggcgt cccctggaac atcatgtgct cgaagaggtg ggcgaagccg gtccgaccct
       61 tcggctcgag gcggaacccg atccggtaga cgacgcagag gccgaacgtc ggggacgaac
      121 ggtcctccga gacgacgacc gtcaggccgt tcggcagctt cctcaccgac gtcgggacct
      181 gccagccggc acccttcggg gccgcggcgg cgggaagggc gaaggacagg accgccacgg
      241 cggccccgaa agcgcgcatc cgtccggaac gggttctcat cgttcctcct tgcatgggaa
      301 gcccacggca gtccgatacg cgccccgcgg ccccggggtt cggacggggg cccgcccccc
      361 ccgggaggag ggtgtgcgag aatccgcccg cgcggcgcgg ccgcgcggtt ctttgagcga
      421 ctggcagccc tgcgcttcgg gctgccggca tgaagagtgg cggagagagc accggctcga
      481 cgacccgccc gccagaccgt agtccctgcg gtggcaacgc cggcccgacc cgcctggatc
      541 gggagccatg cgcgatcgaa gcgtgacctg catcgtcgaa aggacgctcc tgaacggatc
      601 cggcacgtcc caagaaccga catttcgatg ttgcgacgga gtttctcgac ggggcgcgtc
      661 cggacgcgcc ccgtcgcggg agcgcgcgcc cgacagacga atcgatacga acgaaaggag
      721 atccaaatga ccagcgaaac cgacttcctc ctgacttccg agtccgtcac cgaggggcac
      781 cccgacaagg tctgcgacct gattgccgac gcggtcctcg acgcgaacat cggccccgac
      841 gagaacgccc gcgtcgcgtg cgaggtcctc tgcaagaagg acaaggtcgt cctggccggc
      901 gagatcacct cgaaggcggg cctgaagaag gccgactacg aggagctcgt ccggggcgtc
      961 atcgagggga tcgggtacgt cgacccggcc gaggccttca acgcgaaggg cgtcaaggtc
     1021 ctcaacttcc tgacccgcca gtcgaaggag atcggcggcg ccgtgaagaa ggcgacggac
     1081 gcgaagaaga aggaccaggg ggccggggac caggggatca tgttcggcta cgcgacgaag
     1141 gagacggacg agctgctgcc gctcccgatc gtactcgcgc accgcctcac gcgcggcctc
     1201 gcggaggccc ggcgcgactt cgtgccgtgg ctcaggcccg acggcaagtc gcaggtgacc
     1261 gtccgctacg agggggggct gccgaagcag gtcgagtcgg tcctcgtctc gacgcagcac
     1321 agccccgacg tgacgcgcga gcagatcgcc gcctgggtgg agaaggacct cgccccgaag
     1381 gccctcggcg agtggatgcg cgacggcatc cgcttcatcg cgaacccctc cggctcgttc
     1441 gtcctcggcg ggccgaccgc cgacgccggc gtaaccggcc ggaagatcat cgtcgacagc
     1501 tacggcggcg cggcccggca cggcggcggc gcctggagcg gcaaggaccc ctcgaaggtc
     1561 gaccggagcg gcgcctattt ctgccggtgg gccgcccggc aggtcgtgaa ggcgggcctc
     1621 gcccgccgcg ccgagatcca ggtctcgtac gcgatcggcg aggcgaagcc gatgtcggtg
     1681 acggtggaga cgttcggaac gggcgacgcg aaggccgctg cggccctcgt ctcgtcgttc
     1741 gacttccgcc ctgccgcgat cagcgagcgg ctgggactgc ggaagccgat ctaccggaag
     1801 acgacgaact acggccactt caagccgggg ctcccctggg aggagtgatc gcgtcctgac
     1861 gcggaggccg cggcgccgcc ctcgggcggg ccgcggcctc ccgggccttc ccctgcgcgg
     1921 ggctcgggga ggcggcgacc tcgcgtcccc gggcgctacc atgcccgcat ggcggtgaag
     1981 gacaggcccc tcttccggcg gatcctcgag ttcgacgacc tcctccggcg gcgccgcgtc
     2041 gtgaacgcga cgaatctcgc gacccgctgg aagacctcga cgaagaccgt gcagcgcttc
     2101 gtcgcgttca tgcgcgacga gatggacgcg ccgatcgtct tcgacaggaa gagcggctcg
     2161 ttccgctact ccgaccccgc ctggcacctc ccctggatcc ccgtcgaagg gcccggcctc
     2221 ttcgcgatcg gcgtcgccgc caaggtgctc cagctctacg aggggacgcc cgtcgccgag
     2281 gggctccgcg aggcgttcga gcgactctcc gacgtgatgc cgccggagat ccgcgtcagc
     2341 cccggcgcgt tcgtcgagcg gctctacatc cacccgcagc cgatccgact cgtcgacccg
     2401 gccgtctgga acgccgtggc gacggcgatc cgcgagaaga cggtcctcgc cgtccgctac
     2461 cagaagcctt ccggcgagac gagcgcgcgc cggctcgcgc cgtacggcct cgtcctcgcg
     2521 gcgggcgact ggctcgtcgc cgcgcaggac cgcgacgacg accccggagt cgtgaagacg
     2581 ttctacgtct cgcgcatccg cggcgccgag acgacccccg agccgttctc cgtcccccga
     2641 gacttcgacc tgaagcgcca cttcggcgag acgatcggga tcttcgtcgg gaaggagccg
     2701 ttccgcttcc gcgtccgctt cacgaaagcg atggcggcgt gggtggccga ggttcgctgg
     2761 catccgaagc agaagctgac gacgctcccc tc
//
LOCUS       DAOTEZ010000515         2778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_28866_length_2778_cov_5.746236, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000515 DAOTEZ010000000
VERSION     DAOTEZ010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28866_length_2778_cov_5.746236"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="P5164_18705"
     CDS             <1..830
                     /locus_tag="P5164_18705"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY45969.1"
                     /translation="PGDEVVVPQGIFRMFPVAVGRAGGTVVEVPTKAGYVPDLDALKR
                     RIGPATKVVAIANPNNPTGAYVPKAELERFFEGLPEHVVAVVDEAYHEFAREVADYPN
                     ALDELRKGRNVLVLRTFSKAYGLAGFRIGYGFTTPEIAAAINKVREPFNTTVVAQVAA
                     IAALDDDEHCERTVSLVRTERAFLATELARRQAVVNPSLGNFLLVELPVPFGPLEPEF
                     ARRGVILRPMGGWGFPLAFRVSVGTHEENLRFLAAFDDLLAAGKLGLSTAAVPVGAG"
     gene            827..1501
                     /gene="cmk"
                     /locus_tag="P5164_18710"
     CDS             827..1501
                     /gene="cmk"
                     /locus_tag="P5164_18710"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRY45970.1"
                     /translation="MIVVAIDGPSGVGKSTVGKALAASLGVPYLDTGAMYRAVGLLAR
                     RKGLALPITEADDAGALAEQAEILVTGSGGDVRTFLDGEDVSAEIREPEISRYASAVA
                     AIPRVRRRLAARQRELALAGGGVLEGRDIGTRVVPEAAAKFFLTARPEVRARRRYEEL
                     VRKGTPQELVAVRAEMEARDAADSSRADSPLSCDGTYVVVDTSDLSAGEVVERLRQLV
                     LGPGAG"
     gene            1594..>2778
                     /locus_tag="P5164_18715"
     CDS             1594..>2778
                     /locus_tag="P5164_18715"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRY45971.1"
                     /translation="MPSEDQKITPIRKTEEAGDGNGEFARLLAESERSLAEGELIRGR
                     VIQVTNDEILVDIGYKSEGMIPRSEFNHSPDRLPQAGQEIDAIVDRREDASGYVVLSH
                     EKARRIRAWDAIEVAFKDNVAVKGKVLERVKGGLSVDIGVRAFLPGSLADIRPLKNLD
                     VLRGRELEFRIVSFDKKRGNIVLSRKALMEEVAEAKKSEAAQALADGRTLHGVVKNLT
                     EYGAFVDLGGIDGLLHVTDISWGRLGHPSEALHVGQEVEVKVLKFDAESGRISLGMKQ
                     LKPDPWQDLPAHYPVGARVRGKVVSLTDYGAFIELEEGVEGLIHVSEMSWTKKVKNPA
                     KLLSVGDTVEAVIADVNRIYGSSFTHADAFCPARSQRIAALYLAHYAPRARAAAPAGM
                     SDA"
BASE COUNT          421 a          990 c          963 g          404 t
ORIGIN      
        1 acccgggcga cgaggtcgtc gtcccgcagg ggatcttccg gatgttcccg gtcgccgtag
       61 gccgggccgg cggcacggtc gtggaggttc ccaccaaggc cggatacgtc ccggacctcg
      121 acgcgctgaa gcggcggatc ggtcccgcca cgaaggtcgt cgcgatcgcg aacccgaaca
      181 acccgaccgg ggcctacgtt ccgaaggccg agctggaacg cttcttcgaa ggcctgccgg
      241 agcacgtcgt cgccgtcgtc gacgaggcct accacgagtt cgcgcgggag gtcgccgact
      301 atccgaacgc cctcgacgag ctccggaagg ggaggaacgt cctggtcctc cggacgttct
      361 ccaaggcgta cggtctcgcc ggcttccgga tcggctacgg attcaccacg cccgagatcg
      421 ccgcggcgat caacaaggtg cgggagccgt tcaacacgac cgtcgtggcc caggtcgccg
      481 ccatcgccgc cctcgacgac gacgagcact gcgagcggac ggtctcgctc gtccggacgg
      541 agcgggcgtt cctcgcgacc gagctcgccc ggcggcaggc cgtcgtcaac ccgtcgctcg
      601 gcaacttcct cctggtcgag ctcccggtcc cgttcgggcc gctcgagccg gagttcgccc
      661 gccggggcgt gatcctgcgc ccgatggggg gatggggctt tccgctggcg ttccgcgtct
      721 cggtggggac tcacgaagag aacctccgct tcctcgcggc gttcgacgac ctcctcgcgg
      781 ccggcaagct cgggctgtcg acggccgccg tccccgtcgg cgccggatga tcgtcgtcgc
      841 catcgacggt ccctccggcg tcggcaagtc gacggtgggg aaggccctgg ccgcgagcct
      901 cggcgttccc tacctcgaca ccggcgcgat gtaccgtgcg gtcggccttc tcgcgagacg
      961 gaaggggctg gccctcccga tcaccgaggc ggacgacgca ggcgccctgg cggagcaggc
     1021 cgagatcctc gtgaccgggt ccggaggcga cgtccggacg tttctcgacg gggaggacgt
     1081 ttccgccgag atccgggagc cggagatctc gcgctacgcc tcggccgtcg ccgcgatccc
     1141 ccgggtccgg cggcgcctcg cggcccggca gcgcgagctg gccctcgccg gaggcggcgt
     1201 cctcgaaggg cgggacatcg ggacgcgggt cgtccccgag gcggccgcga agttcttcct
     1261 cacggcgcgg cccgaggtgc gggcccgaag gcggtacgag gagctcgtcc ggaaggggac
     1321 tccccaggag ctggtcgccg tccgcgccga gatggaggcg cgcgacgcgg ccgactcgtc
     1381 ccgtgccgac tcgccccttt cctgcgacgg gacctacgtc gtcgtcgaca cctccgacct
     1441 ctcggccgga gaggtcgtgg agaggctccg gcagctcgtc ctcggccccg gagccggttg
     1501 acctgtcgaa gcctcccgtc taacattccc ggttcgggcc gctaactcaa gacccttgag
     1561 ccgtctccgc gagccctcga gaaggtggaa ttcatgccgt cggaggacca gaagatcacg
     1621 cccatccgga agacggagga ggctggcgac gggaacggag agttcgcgcg cctgctcgca
     1681 gagagcgagc gttccctcgc cgagggagag ctgatccgcg ggcgtgtcat ccaggtcacg
     1741 aacgacgaga tcctcgtcga catcggctac aagtcggagg ggatgatccc gcggtcggag
     1801 ttcaaccact cgccggatcg cctcccgcag gccggacagg agatcgacgc gatcgtcgac
     1861 cgtcgcgagg acgcttcggg ctacgtcgtc ctctctcacg agaaggcgcg ccgcatccgc
     1921 gcctgggacg ccatcgaggt ggccttcaag gacaacgtgg cggtcaaggg caaggtcctc
     1981 gagcgcgtga agggcggcct ctcggtcgac atcggcgtgc gggccttcct gccggggtcg
     2041 ctcgccgaca tccgccccct gaagaacctc gacgtcctcc gcggccgcga gctcgagttc
     2101 cgcatcgtca gcttcgacaa gaagcgcggg aacatcgtcc tctcgcgcaa ggctctgatg
     2161 gaggaggtcg ccgaggcgaa gaagtccgag gccgcccagg cgctcgccga cggccgcacg
     2221 ctccacggcg tcgtgaagaa cctgaccgag tacggcgcgt tcgtcgacct gggcgggatc
     2281 gacgggctcc tgcacgtgac cgacatctcg tggggccggc tcgggcatcc gtccgaggcc
     2341 ctgcacgtcg gccaggaggt cgaggtgaag gtcctcaagt tcgacgcgga gtcgggccgg
     2401 atctccctcg gcatgaagca gctcaagccc gatccgtggc aggacctccc cgctcactac
     2461 ccggtcgggg cccgcgtccg cggcaaggtc gtctccctca ccgactacgg cgccttcatc
     2521 gagctggagg agggggtcga aggcctcatc cacgtcagcg agatgtcctg gacgaagaag
     2581 gtcaagaacc ccgccaagct cctctcggtg ggggacaccg tcgaagcggt catcgccgac
     2641 gtcaaccgca tctacggctc ctcattcacc cacgccgacg ccttctgccc ggcccggtcg
     2701 cagcggatcg cagcgcttta cctggcgcac tacgcgcccc gcgccagggc ggccgcgccg
     2761 gcaggcatga gcgacgcc
//
LOCUS       DAOTEZ010000516         2775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_28904_length_2775_cov_6.148162, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000516 DAOTEZ010000000
VERSION     DAOTEZ010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28904_length_2775_cov_6.148162"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="P5164_18720"
     CDS             complement(<1..195)
                     /locus_tag="P5164_18720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45972.1"
                     /translation="MHKRVVSAAAGLALVLGCAGIAVAADEAKPLYVQTIQKYRAPVL
                     DDVGGREYKFLIDPAKTKGKP"
     gene            complement(408..1757)
                     /locus_tag="P5164_18725"
     CDS             complement(408..1757)
                     /locus_tag="P5164_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012075434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRY45973.1"
                     /translation="MPEPKVLLIEDDPAVRHGMAAFLRANGLDVDEADTCQKAIERFR
                     AVGHDVVVADYSLPDGTSLDILPQVKKLSEETPFVILTAHGSIDLAVRAIKDGAEQFL
                     TKPVESKALLVLVKRLLQQQRLRKRQELVARTQPGILDPFVGTSPQVRRLDERARLML
                     EWDSPVLILGETGSGKGVLARWIHAHGPRREEAFVNLNCAGLTRELLESELFGHERGA
                     FTGAVNPKPGLLEVGHRGIVFLDEIGDMDPAIQPKLLKALEEKTFRRLGDVRDRHVDI
                     RLVASTNLDLEAMVREKKFRDDLYYRISTLTLRIPPLRERPEDILVLARNILAGVCGR
                     MGRAPARLTQDAERVLQRYPWPGNIRELANVIERSMILVKGETLDAAGLGLDAVAAAP
                     PREHDRLLTMKEMERLHVERVLAKTGGNVTEAAAILGMARRTLYDRMRSLGLGGARE"
     gene            complement(1750..>2775)
                     /locus_tag="P5164_18730"
     CDS             complement(1750..>2775)
                     /locus_tag="P5164_18730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HRY45974.1"
                     /translation="GLWDWDLSTGKVYRSPRWFGMIGLTPGGVEDTFDAGLRLIHSDD
                     RRQIEAAMSAHLEGRVPRFEAEYRLRTRTGEWIWVFDAGKVVAWDGDRPARVAGMCTD
                     ITERRRAEEALRALVVGVVHEIRNPVYGILINLDALEATFGEDPRYVSFVAALRESAE
                     RIASLVNDLRDYGEPRTLNPEPHLVRSLVENAVRACDKLAADRSCGVAVELEDPSLVL
                     PLNPRRMHQVFRNLLENALLHSPEDSTVRIAGRRTFSDGFYWWTFDVEDSGGGFDAET
                     LSRGFEPFFTRRKGGTGLGLSIVKRVVEEHGGSVEAANRPEGGARVTVRLPAPHQPLQ
                     AAGGARA"
BASE COUNT          369 a         1006 c          978 g          422 t
ORIGIN      
        1 gggcttcccc ttcgtcttcg ccgggtcgat caggaacttg tactcgcgcc cgcccacgtc
       61 gtcgagcacc ggggcccggt acttctggat ggtctggaca tagagcggct tcgcctcgtc
      121 ggcggcgacg gcgatgccgg cgcagccgag gacgagcgcc aggcccgctg cggccgagac
      181 gactctcttg tgcattccca accctcctcg cgactctctc ccggcctccc gcggccccgg
      241 gcgattcccg gctcccggaa ggcatccggt ctcccggtcc gtttgcaacc gacgggccat
      301 ccgcccggcg gcgccgggac cgcgcagctc tgccctcccg tacgcagccg cgcgatccgg
      361 aacgcgcgcc gcgtgcggga tcccgcacgc ggcgtgcgga gaaccgctca ctccctcgcg
      421 ccgccgagac cgaggctcct catccggtcg tagagcgtcc ggcgggccat cccgaggatc
      481 gcggcggcct ccgtgacgtt ccctcccgtc ttcgcgagga ctcgctcgac gtggagccgc
      541 tccatctcct tcatcgtcag gagccggtcg tgttcgcgcg gcggggccgc cgcgacggcg
      601 tcgagaccga ggcccgccgc gtcgagcgtc tcgcccttca cgaggatcat cgagcgctcg
      661 atgacgttcg cgagctcgcg gatgttcccg ggccacgggt agcgctgcag gaccctctcc
      721 gcgtcctgcg tgaggcgggc cggcgcgcgg cccatccggc cgcagacgcc cgcgaggatg
      781 ttccgagcca ggacgaggat gtcctcgggc ctctcgcgga gcggcgggat ccggagcgtg
      841 agcgtcgaga tgcggtagta gaggtcgtct cggaacttct tctcgcggac catcgcctcg
      901 aggtcgaggt tcgtgctcgc gacgagccgg atgtcgacgt gccggtcgcg gacgtcgccg
      961 aggcggcgga acgtcttctc ctcgagcgcc ttcaggagct tcggctggat cgccgggtcc
     1021 atgtcgccga tctcgtcgag gaagacgatc ccgcggtgcc ccacctcgag gagcccgggc
     1081 ttagggttca ccgcgcccgt gaaggcgccg cgctcgtggc cgaagagctc cgactcgagc
     1141 agctcgcgcg tcagccctgc gcagttgagg ttgacgaagg cctcctcgcg gcgcggcccg
     1201 tgggcgtgga tccagcgcgc gaggacgccc ttgcccgagc cggtctcgcc gaggatgagg
     1261 accgggctgt cccactcgag catgaggcgg gcgcgctcgt cgagccgcct cacctgcggg
     1321 ctcgtcccga cgaacgggtc gaggatcccg ggctgcgtcc gcgcgacgag ctcctgccgc
     1381 tttcggaggc gctgctgctg caggagccgc ttgacgagga cgaggagcgc cttcgactcg
     1441 accggcttgg tgaggaactg ctccgcgccg tccttgatcg cgcggacggc gaggtcgatc
     1501 gagccgtgcg ccgtcaggat gacgaacggc gtctcctcgg agagcttctt gacctgcggc
     1561 aggatgtcga gcgaggtgcc gtcggggagg ctgtagtcgg cgacgacgac gtcgtgcccc
     1621 acggcgcgga agcgctcgat cgccttctgg caggtgtccg cctcgtcgac gtcgaggccg
     1681 ttcgcccgga ggaaggcggc catcccgtgc cgcacggcgg ggtcgtcctc gatcaggagg
     1741 accttgggct caggcacgcg cgcctcccgc cgcctggagc ggctggtgcg gggcgggcag
     1801 gcgaaccgtg acacgggccc cgccctccgg gcggttcgcc gcctcgacgc tcccgccgtg
     1861 ctcctcgacg acccgcttga cgatggagag cccgagcccg gtccccccct tcctccgggt
     1921 gaagaacggc tcgaagcccc gcgagagggt ctccgcgtcg aagccgccgc cggagtcctc
     1981 cacgtcgaac gtccaccagt agaagccgtc cgagaacgtc ctgcggccgg cgatgcgcac
     2041 cgtcgagtcc tccggcgagt ggaggagcgc gttctcgagc aggttccgga agacctggtg
     2101 cattcgccgg ggattgagag ggaggacgag cgacggatcc tcgagctcga cggcgacgcc
     2161 gcacgagcgg tcagcggcga gcttgtcgca cgcccgcacg gcgttctcga cgaggctcct
     2221 cacgaggtgg ggctccgggt tcagcgtccg cggctcgccg tagtcgcgaa ggtcgttcac
     2281 gaggctcgcg atccgctccg cgctctcccg gagcgccgcg acgaaggaga cgtagcgcgg
     2341 gtcctcgccg aacgtcgcct cgagcgcgtc gaggttgatg aggatgccgt agacggggtt
     2401 gcggatctcg tggaccacgc cgacgacgag cgcacgcagc gcctcctcgg cgcgccgcct
     2461 ctccgtgatg tcggtgcaca tgccggcgac tcgggcggga cgatcgccgt cccaggcgac
     2521 gaccttcccc gcgtcgaaga cccagatcca ctcgccggtc cgcgtcctca ggcggtactc
     2581 cgcctcgaat cgagggaccc gtccctcgag gtgcgcgctc atcgccgcct cgatctgcct
     2641 ccggtcgtcg gagtggatca gccggagccc ggcgtcgaac gtgtcctcga cgccgccggg
     2701 cgtcaggccg atcatcccga accagcgggg actcctgtag accttcccgg tcgacaggtc
     2761 ccagtcccac agccc
//
LOCUS       DAOTEZ010000517         2757 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_29198_length_2757_cov_5.009993, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000517 DAOTEZ010000000
VERSION     DAOTEZ010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29198_length_2757_cov_5.009993"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /locus_tag="P5164_18735"
     CDS             <1..1132
                     /locus_tag="P5164_18735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRY45975.1"
                     /translation="KALAKNPQDRYQNASEMERDINSVLYSFQPAPGPADLAIFLHRL
                     VEATPVASDAQIDAAFAQAAATAPAPEEKKKGKGLVISKKEKVPEPLPVPEPVEVSPP
                     AISVDEEAGKSRTGLFAGIGVAAVVLIAAAVFLTRGKKEEPAPAPVAAPTAAAEPAPA
                     TTEAPAAPEKVIDAKALEAELKKATAEEAKKLREAAQKAAAEQQAKPASGAPAAAPAV
                     SLAPSQPLQPPKPEATATPVPAPTRAPEPTPVPVRPTEPPRAVEAPRPAAPAPAAPAA
                     AAVREGDLVGAGPGVQEARLVKLGAFPRLPAQARRVTGPAAIMALVDENGRVTSTRVV
                     KSSGNPIADEAAVSALKGAQIEPATKTGVRVKMWKTFSITVK"
     gene            1152..1373
                     /locus_tag="P5164_18740"
     CDS             1152..1373
                     /locus_tag="P5164_18740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45976.1"
                     /translation="MSLSDDQRKFYENTLRVTKKEISDLEANIQEELAKVKERLAELQ
                     NGQKAARQMYAAACLRLGIQNDLEESEES"
     gene            1468..2322
                     /locus_tag="P5164_18745"
     CDS             1468..2322
                     /locus_tag="P5164_18745"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HRY45977.1"
                     /translation="MRKPRLLLAAVAVACAFVAPPLAAAPPRRVVVLAPSAAEIVFAL
                     GAADRVVGVSDFAAGLPGAEGKERLGGFVPDLERIAALAPDLAVVSRDGTDRRAAERL
                     AALGVRVVVTDAASLDGVYADVRRVGAALGRAAEAERIVDRMQRRVALAEERVRLRRG
                     ARRPTALAVIWPDPPVVAGRATFVGDLLERAGLDNVVPRSAGEWPRVSHETLVGWNPR
                     LLVRPDTRENRAAFEAALAPASRWQLLPAVRAGRVVVLDGDWLERPGPRLVDALEALV
                     EELRAGTP"
     gene            2319..>2757
                     /locus_tag="P5164_18750"
     CDS             2319..>2757
                     /locus_tag="P5164_18750"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HRY45978.1"
                     /translation="MRRTGRFLALLALLAFGAVAASLLVGSVPIAPADALAALAGDGD
                     AATRTIIRDLRLPRALLGLLVGGALALSGAALQALLGNPLADPYLLGVSGGAACGTLG
                     AVVLGLGTAGLFGSFALPLASLAGALGAVLLVGLGAAVGGRLDR"
BASE COUNT          334 a         1093 c          996 g          334 t
ORIGIN      
        1 caaggcgctc gcgaagaacc cgcaggaccg ctaccagaac gcctcggaga tggagcggga
       61 catcaactcg gtcctctact ccttccagcc ggcgccggga ccggccgacc tcgcgatctt
      121 cctccaccgc ctcgtcgagg cgaccccggt cgcctccgac gcgcagatcg acgcggcgtt
      181 cgcgcaggcg gccgcgacgg cgcccgcgcc cgaggagaag aagaagggga aggggctcgt
      241 catctccaag aaggagaagg tccccgagcc gcttcccgtc ccggagcccg tcgaagtctc
      301 cccgccggcc atcagcgtgg acgaagaggc ggggaagagc cgcacggggc tgttcgccgg
      361 catcggcgtc gccgccgtcg tcctcatcgc cgcggccgtc ttcctgacgc gtggcaagaa
      421 ggaggagccg gccccggccc ccgtcgccgc tccgaccgcg gcggccgagc ccgccccggc
      481 gaccaccgag gcgccggccg cccccgagaa ggtcatcgac gccaaggcgc tcgaggccga
      541 gctgaagaag gcgacggccg aggaagcgaa gaagctgcgc gaggccgcgc agaaggcggc
      601 ggccgagcag caggcgaagc cggcttccgg cgccccggcg gcggctccgg ccgtttccct
      661 cgcgccgagc cagccgctcc agccgccgaa gcccgaggcg acggcgaccc cggtcccggc
      721 gccgacgcgg gcccccgagc cgaccccggt ccccgtccgc ccgaccgagc ctccgcgcgc
      781 cgtcgaggcg ccgcgccccg cggctccggc cccggcggcc cccgccgcgg ctgccgttcg
      841 cgagggcgac ctcgtcggcg cgggcccggg cgtccaggag gcccggctcg tcaagctcgg
      901 ggcgttcccg cggctccccg cccaggcgcg gcgcgtgacg ggtccggccg cgatcatggc
      961 cctcgtggac gagaacggac gcgtcacctc gacacgtgtc gtcaagtcgt ccggcaaccc
     1021 gatcgcagac gaagcggccg tctcggccct caagggggct cagatcgagc ccgccacgaa
     1081 gaccggggtc cgcgtgaaga tgtggaagac gttctccatc acggtgaagt agctcgagaa
     1141 ggagttccgg catgagcctc agcgacgatc agaggaagtt ctacgagaac acgctccgcg
     1201 tcacgaagaa ggagatcagc gacctggaag ccaacatcca ggaagagctg gccaaggtga
     1261 aggagcgcct cgccgagctt cagaacggcc agaaggccgc gcggcagatg tacgccgccg
     1321 cgtgcctccg cctcgggatc cagaacgacc tcgaggagtc cgaggagtcc tgacgggcgg
     1381 cgggcgcccg ccgtccgatc gaccgcggac cggccccggc gactccctcg cgtgagggcg
     1441 ccggggcctt cgccttctct ctcgaccttg cgcaagcctc gactcctgct cgccgcggtc
     1501 gccgtcgcgt gcgccttcgt cgcgccgccc ctcgccgcgg cgccgccccg ccgcgtcgtc
     1561 gtcctcgccc cgtccgcggc ggagatcgtc ttcgccctcg gggcggcgga cagggtcgtc
     1621 ggcgtttccg acttcgcggc cggccttccc ggcgcggagg ggaaggagcg gctcggcggc
     1681 ttcgtcccgg acctcgagcg aatcgcggcg ctcgcacccg acctcgcggt cgtctcgcgg
     1741 gacggcaccg accgtcgagc cgccgagcgg ctcgccgcgc tcggcgtccg cgtcgtggtc
     1801 accgacgcgg cctctctcga cggcgtctac gccgacgtcc ggagggtcgg cgccgccctc
     1861 ggccgggccg ccgaggccga gcggatcgtc gaccggatgc agcgacgggt cgcgctggcg
     1921 gaggagcgcg tccgccttcg ccgtggcgcg cgccggccga cggcgctcgc ggtgatctgg
     1981 cccgacccgc cggtcgtggc ggggcgggcg acgttcgtgg gcgatctcct cgagcgcgcg
     2041 ggcctcgaca acgtcgtccc gaggagcgcc ggcgaatggc cgcgggtctc gcacgagacg
     2101 ctcgtgggct ggaacccccg cctcctcgtc cggcccgaca cacgggagaa ccgggccgcg
     2161 ttcgaggcgg cgctcgcgcc ggccagccgc tggcagctcc tcccggcggt ccgggcgggg
     2221 cgtgtcgtcg tcctcgacgg cgactggctc gagcgccccg ggccgcgcct cgtcgacgcc
     2281 ctcgaagcgc tcgtcgaaga gctccgggcg gggacgccgt gaggaggacg ggccgcttcc
     2341 tcgccctcct cgcgctcctc gccttcggcg cggtcgccgc gtccctcctc gtcgggagcg
     2401 tcccgatcgc gccggccgac gccctcgccg ccctcgcggg ggacggtgac gcggccacgc
     2461 ggacgatcat tcgcgacctg cgccttcccc gggccctcct ggggctcctc gtcggcgggg
     2521 cgctcgccct ctcgggcgcc gcgctccagg cgctcctcgg caaccccctc gccgatccgt
     2581 acctcctcgg cgtctcgggg ggagccgcct gcggcacgct cggcgccgtc gtcctcggcc
     2641 tcggcaccgc ggggctcttc gggtcgttcg ccctcccgct cgcctccctc gcgggggcgc
     2701 tcggcgccgt cctcctcgtc ggcctcggcg ctgccgtcgg gggccggctc gaccggg
//
LOCUS       DAOTEZ010000518         2747 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_29340_length_2747_cov_4.898217, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000518 DAOTEZ010000000
VERSION     DAOTEZ010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29340_length_2747_cov_4.898217"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..>2747
                     /locus_tag="P5164_18755"
     CDS             149..>2747
                     /locus_tag="P5164_18755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45979.1"
                     /translation="MRLAVPLLLAASLAAPAAARVVSYAPVTNRYATPIHQPRASDRF
                     VLAESDVPSTWYYSSTPRGRLVIHDATGREEPRVVLPTTGPEVLFSAAGARPGSDGIL
                     RILALVDRSPQGVEDHSKKQLLFSRDGGETWVEVLLPSGVLPVVAVATSPVADFGGPN
                     ARGRQPSLRLGSDTTPFFLCLQKSAYGDDRAVVAVDDDGAARLLTESSMVTWPDGQGS
                     QLGYFPRFVGTSHDGASVLMLGGLPVPGAPGQWSAGLWRVGASGGVERLAELPGGLPS
                     IEAWLTPGGLAYLDVDPWSGPTPAAPFHEGQGLYLVEGGTVKRVAGPGGDPGGSGQTG
                     TELIAIPTSDFEGAWISRRGLGRPTTLARHTPGGGLVEMWSDPTAPEVEALHAGASGR
                     RLLVQVHRPRPQMDQRLILDPALAVWEAGQPAPARYDELFLNEQPTKGFVHLDVEAVA
                     SGQPFVFDAGPLEGWCCVGGGPSGDGGSGGADVFQEWGVVKGSLRQRLVIPAVARTGG
                     EGGAFWKTDLVLGNPGPDPLSVDLRFVPADEGDSANATLTLDPNEIRVVADVLDSLFG
                     RNAGSGALFLTPEGTRVVSATTRTYTASEEGSYGMSLGAVDLFAASSARFPLAFSGAF
                     PGWGYRTNAGAVDVAGRGATVGLRFASESGWTGRSDLFVEARPDGTAQLNRLGTWLGV
                     EPWRAGALKYAPARGESIPFVTSIDETTNDPTAWRPDLPAPVARAIPALVHADGRNGA
                     RFRSDLFLYNDSDAPASVTLAAKRWDSTAPEKLVTLTLFPREAKAVRDALAAVFQLDG
                     VARLRFLSGEGGAGLGVRVTSRAYTVRPDGGTYGVPLPPLNAFQMAGAGEALELFGVL
                     GGPSFRTNLA"
BASE COUNT          336 a          980 c         1018 g          413 t
ORIGIN      
        1 tggaagaagc gggagctgcc ggggccgggg gcgcccgggg tggaggtcgc cgcggcggcg
       61 tgagaagcct cgcgccgaag tcacttcagg cgtaggtcac cggccgggcc cagcttcgtc
      121 tggtcccggc cgggtccggg gaggttccgt gcgccttgct gttccgctgc tcctcgcggc
      181 ctctctcgcg gcgcccgcag ccgcgcgcgt cgtttcgtac gcccctgtca cgaacaggta
      241 cgcgacgccc atccaccagc cccgggcgag cgaccgcttc gttctggccg aatcggacgt
      301 tccgtccacc tggtactact cctcgactcc ccgcgggcgc ctcgtgattc acgacgcgac
      361 gggccgggag gaaccgcggg tcgtcctgcc gacgaccggg cccgaggtgc tcttctcggc
      421 cgccggcgcg aggccgggtt ccgacgggat cctccgaatc ctggcgctcg tcgatcggag
      481 cccccagggg gtcgaggacc actcgaagaa gcagctcctc ttcagccgcg acgggggcga
      541 gacctgggtc gaggtcctcc ttccctccgg agtcctcccc gtggtcgcgg tggcgacaag
      601 cccggttgcc gatttcgggg gaccgaacgc tcggggccgg cagccctcgc tgaggctcgg
      661 ctcggacacg acgccgttct tcctctgcct ccagaagtcg gcgtacggag acgaccgggc
      721 cgtcgtggcc gtcgacgacg acggagcggc gcggctcctg accgagtcgt ccatggtcac
      781 ctggccggac ggacagggtt cgcagctcgg gtacttcccg cgcttcgtcg gcacgagcca
      841 cgacggcgcc tcggtcctga tgctcggagg cctcccggtt cccggagcgc cgggccaatg
      901 gtcggcgggt ctctggcgcg tcggcgcctc cggcggcgtg gaacggctcg ccgagcttcc
      961 gggcgggctc ccatcgatcg aggcctggct cacgcccggc ggcctcgcct acctcgacgt
     1021 cgatccgtgg tccggtccga cgccggcggc gccgttccac gaagggcagg ggctgtacct
     1081 ggtcgagggc gggactgtga agcgggtcgc cgggcccggc ggcgatcccg gcggctcggg
     1141 ccagacgggc acggagctga tcgcgattcc gacgtccgac ttcgagggcg cctggatctc
     1201 acgccgcggc ctcgggcggc cgaccacgct ggcccggcac acgcccggcg gcgggctcgt
     1261 cgagatgtgg agcgacccga cggcccccga ggtcgaggcg ctgcacgcgg gcgcctccgg
     1321 tcggaggctc ctcgtgcagg tccaccggcc gcgcccgcag atggaccagc gcctcatcct
     1381 cgatccggcc ctggccgtct gggaggcggg gcagcccgca ccggcccgct acgacgagct
     1441 cttcctgaac gagcagccga cgaaggggtt cgtccacctc gacgtcgagg cggtggcgtc
     1501 ggggcaaccg ttcgtcttcg atgccggacc cctggagggg tggtgttgcg tcgggggcgg
     1561 accgtccggc gacggaggga gcggcggagc cgacgtgttc caggagtggg gcgtcgtcaa
     1621 ggggtcgctc cggcagcgcc tcgtcattcc ggccgtcgcc cgaaccgggg gcgaaggcgg
     1681 cgcattctgg aagacggacc tggtcctcgg gaatccgggc ccggacccgc tgtccgtgga
     1741 ccttcgtttc gttcccgccg atgaggggga ttctgcgaac gcgacgctga ccctcgaccc
     1801 gaacgagatc cgggtcgtcg cagacgtcct cgattcgctc ttcgggagga acgccggctc
     1861 gggggcgctc ttcctcactc ccgaggggac ccgtgtcgtc tccgcgacga cccgcaccta
     1921 cacggcctcc gaggaggggt cctacgggat gtcgctcggt gcggtcgacc tcttcgccgc
     1981 gtcgagcgcc cggttcccgc tcgcgttttc gggagccttt cccggctggg gatacaggac
     2041 gaacgcgggg gcggtcgacg tcgccggacg gggcgcgacc gtcggcctga ggttcgcttc
     2101 ggagtcggga tggacgggac ggtccgacct cttcgtcgaa gcccggcccg acggaacggc
     2161 gcagctgaac cggctgggaa cgtggctggg ggtcgagccg tggcgagcgg gcgccctgaa
     2221 gtacgcacct gcgcgcggcg agtccattcc gttcgtcacg tcgatcgacg agacgacgaa
     2281 cgacccgacc gcctggcgac ccgaccttcc cgcgccggtg gcgcgggcga ttccggcgct
     2341 cgtccacgcg gacggccgga acggcgcgcg cttccggagc gacctcttcc tctacaacga
     2401 ctcggacgcg cccgcgagcg tgacgctggc ggcgaagcgc tgggattcga cggcgcccga
     2461 gaagctcgtg acgctcacgc tctttccgcg cgaggcgaag gccgttcgcg acgcgctcgc
     2521 cgccgtgttc cagctggacg gggtcgcccg gctccgtttc ctgtcgggag agggcggggc
     2581 cgggctcggc gtccgggtca cgtcgcgggc ctatacggtg aggccggacg gcgggacgta
     2641 cggcgtcccg ctccctcctc tgaacgcgtt ccagatggcc ggggccggag aggcgctcga
     2701 gctcttcggc gtcctcggcg gaccctcctt ccggacgaac ctcgcgc
//
LOCUS       DAOTEZ010000519         2743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_29400_length_2743_cov_6.555804, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000519 DAOTEZ010000000
VERSION     DAOTEZ010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29400_length_2743_cov_6.555804"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(402..1175)
                     /locus_tag="P5164_18760"
     CDS             complement(402..1175)
                     /locus_tag="P5164_18760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009334092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45980.1"
                     /translation="MTFTRALVRPPASTFADGLTTAGLGSPDLPLALAQHEAYVKALE
                     GCGLSVTRLPADDRFPDSTFVEDTAVLARGLAVLCRPGAPSRAGEVEAIRTALESFFP
                     SPVAIEAPGTVDGGDVCEAGDHVFVGISERTNEAGARQLAAHLATRGLGTTLVDVRGI
                     PGLLHLKSGIAWLGERTLALARPFAGRPEFAGWDVLDVPAGEEYAANAVLVNGRVLLA
                     SGYPRFETALRALGLRTVAFDMSEFRKLDGGLSCLSLRF"
     gene            complement(1178..2488)
                     /locus_tag="P5164_18765"
     CDS             complement(1178..2488)
                     /locus_tag="P5164_18765"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HRY45981.1"
                     /translation="MTSPDGATPSASSEGFLSNRILLGLLLGAICGVGANLLLVPRFP
                     DAVEAANRWLFVPVGQIFLRMLFMVVIPLVFSSLALGVASLGDLKRLGRVGGRTMLYF
                     LSVTAVSVAIGLVLVNVLRPGEGLPAETREKLLETYRGEAASRMESAGKADFGPSLLV
                     NIVPRNPVAAAANGEMLPVIFFALLFGIGVTKLSAEKNALWMKLLEGLADVTVVIIGM
                     ALKLAPFGVFGLIFSVTSRFGFDILRQLLFFVVTVLLGLSLLILGVYPLYLKFRAGVS
                     PVAFLRKMRPVMITAFSTSSSNATLPTTIEVSERDLGVPPQIAGFVLPLGATMNMNGT
                     ALFEGITCVFLAQVFGIHLSLGQQLFVIVLAVLTAIGTAGVPGGSIPLLVLVLTSVGV
                     PAEGIAVIIGVDRLLDMARTVPNVIGDVTCAVYVAKKEGFELKI"
     gene            2566..2641
                     /locus_tag="P5164_18770"
     tRNA            2566..2641
                     /locus_tag="P5164_18770"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2599..2601,aa:His,seq:gtg)
     gene            2665..2742
                     /locus_tag="P5164_18775"
     tRNA            2665..2742
                     /locus_tag="P5164_18775"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2700..2702,aa:Arg,seq:ccg)
BASE COUNT          473 a          858 c         1062 g          350 t
ORIGIN      
        1 gagagacgga gagacggaga gacggagaga cggagagacg gagagacgga gagacggaga
       61 gaccccgcag gccatctcgc aggggttcac gagtcgcgga cgggaggaac gtcccacggc
      121 gagctctgcg cgcgcgcgac ccgcatcggc cccgctgcgc gggccgccgg cgcggccgcg
      181 aactcctcgc tgcgctcgtc aaacagcgcg gccgcttctt ccggcggcct gctcgcggag
      241 ccgtgcgggt gccccgcacg tgctcggacg ctcgctcgcg ggacgcccct cccgtccgct
      301 cgacgttcgt gtcggatcga gcgatccgcg agggcgggaa tcgcgttgag cgggactcgg
      361 gactgtcgct tgagggagcg aacgcgcagt ttgcggatcg gctagaaccg cagcgagagg
      421 cagctcaggc caccgtcgag cttgcggaac tccgacatgt cgaaggcgac tgtacggagg
      481 ccgagtgcgc ggagggcggt ctcgaagcgg gggtagcccg aggcgaggag gacccggccg
      541 ttcacgagga cggcgttcgc ggcgtactcc tcgccggccg ggacgtcgag gacgtcccag
      601 cccgcgaact cggggcggcc ggcgaagggg cgcgcgagcg cgagggtccg ctcgccgagc
      661 cacgcgattc cgctcttgag gtggaggagt cccgggatcc cgcggacgtc gacgagcgtc
      721 gtcccgaggc cgcgggttgc gaggtgggcg gcgagctggc gggcgcccgc ctcgttcgtc
      781 cgctccgaga tgccgacgaa gacgtggtcg ccggcctcgc agacgtcgcc gccgtcgacc
      841 gtcccggggg cttcgatggc gacgggagag gggaagaacg actcgagggc ggtgcgaatc
      901 gcttcgacct cgccggcccg gctcggcgcg cccggccggc agaggacggc caggccgcgg
      961 gcgaggacgg ccgtgtcctc gacgaacgtc gagtccggga agcggtcgtc ggccgggagc
     1021 cgcgtgacgg agagcccgca cccctcgagg gccttcacgt aggcctcgtg ctgggcgagg
     1081 gcgagcggaa ggtccggaga tccgaggccg gccgtcgtca ggccgtcggc gaaggtcgag
     1141 gccgggggcc tcacgagggc gcgcgtgaac gtcatggtca gatcttcagc tcgaagcctt
     1201 ccttcttcgc gacgtagacg gcgcaggtca cgtccccgat gacgttcggg acggtacggg
     1261 ccatgtcgag gaggcggtcg actccgatga tgacggcgat cccctcggcg gggacgccga
     1321 cggaggtgag gacgaggacg aggagcggga tggagccgcc gggaacgccc gcggttccga
     1381 tcgccgtgag gacggcgagg acgatcacga agagctgctg gccgagcgag aggtggatcc
     1441 cgaacacctg ggcgaggaag acgcaggtga tcccctcgaa gagcgccgtc ccgttcatgt
     1501 tcatcgtggc gccgagcggc aggacgaagc cggcgatctg cggcgggacg ccgaggtctc
     1561 gctcggagac ctcgatcgtc gtcggcagcg tcgcgttcga cgagctcgtg gagaaggccg
     1621 tgatcatcac cggccgcatc ttccggagga acgcgaccgg ggagactccg gcgcggaact
     1681 tgaggtagag cgggtagacg ccgaggatga ggagggaaag tccgaggagg accgtgacga
     1741 cgaagaagag gagctgccgg aggatgtcga agccgaagcg ggacgtgacc gagaagatga
     1801 ggccgaagac gccgaacggt gcgagcttca gcgccatccc gatgatcacg accgtgacgt
     1861 cggcgagccc ttcgaggagc ttcatccaga gggcgttctt ctccgcggag agcttcgtga
     1921 cgccgatgcc gaagaggagc gcgaagaaga tcaccgggag catctccccg ttcgccgccg
     1981 ccgcgacggg gttgcgcggg acgatgttca cgaggagcga cgggccgaag tcggcctttc
     2041 cggccgattc catccgcgag gccgcctcgc cgcggtaggt ctcgaggagc ttctcgcgtg
     2101 tctcggccgg gagcccctcg ccgggccgga ggacgttcac gaggacgagc ccgatcgcga
     2161 cggagacggc ggtgacggag aggaagtaga gcatcgtccg cccgccgacg cgcccgagcc
     2221 gcttcaggtc gccgaggctc gccacgccca gcgcgagcga cgagaagacg agcgggatga
     2281 cgaccatgaa gagcatccgg aggaagatct ggccgacggg gacgaagagc caccggttcg
     2341 cggcctccac cgcgtccggg aagcgcggca cgaggaggag gttcgcgccg acgccgcaga
     2401 tcgccccgag cagcagcccg agcaggatcc ggttcgaaag gaagccctcg gacgaggcgg
     2461 aaggcgttgc gccgtcggga gatgtcatga cgcggaagga tgccaccccg aggggggtgg
     2521 cggtgcgggg cgtttctgat atcctccgcc gtccgaatga gcgcggtgag agtagctcag
     2581 tcggtagagc gccggattgt ggttccggtg gtcgcgggtt cgacccccgt ctctcacccc
     2641 atcatcaccg ctctttcgaa attcgcgccc gtagctcagc ccggatagag cgttagactc
     2701 cggatctaaa ggtcccgcgt tcgaatcgcg gcgggcgcac cac
//
LOCUS       DAOTEZ010000520         2733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_29588_length_2733_cov_4.492532, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000520 DAOTEZ010000000
VERSION     DAOTEZ010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29588_length_2733_cov_4.492532"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="P5164_18780"
     CDS             complement(<1..1080)
                     /locus_tag="P5164_18780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRY45982.1"
                     /translation="MSTETTATPLTADEERIARAKEEWRRRVAESFGKRPAWKRDFTT
                     VSSAEVNPLATPDDVAGFDYARDLAFPGEFPYTRGVQTTMYRGKPWTIRQFAGFGTAR
                     QTNERFKVLLAHGQTGLSTAFHLPTLYGYDSDHEMSAGEVGKCGVAVDTLRDFEVLFD
                     GIDLGSVTTSMTINSTAPIALAMYLAVAEKQGTPWSKVGGTLQNDILKEYIAQKEFIF
                     PPRPSMRLVTDQFAFCAKHVPKWNTVSISGYHIREAGSTALQELAFTLRDGMEYVKYG
                     VDAGLPVDEFAPRLSFFFNAHNDFFEEIAKYRAARRVWAKVMRDRYGAKNPESWKLRF
                     HTQTAGVSLTAQQPYNNVARVAVQAL"
     gene            complement(1179..2126)
                     /locus_tag="P5164_18785"
     CDS             complement(1179..2126)
                     /locus_tag="P5164_18785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HRY45983.1"
                     /translation="MSGARAGSGSAERGGVVLSDEVQAALAERRGVVALESTILCHGL
                     PYPKSLETHRAAEAAVRREGAVPALVAVVDGVPRAGFDDAGAEALARAGSRVRKATTR
                     DLGLLAARGESGATTVAATMAIAAAAGIRVFATGGIGGVHRGAEETFDVSADLLELSR
                     TRVVVVAAGAKAVLDLPKTLEALETLGVLVVGFGTAELPAFWMRGSGLPLEVVCDDAA
                     EVSRLAAAHWAWPSSGGILVANPVPAAAALPREVVEGAIERSLAAACAEGVVGKAVTP
                     FLLARLAEETGGRTLAANEALVVSNAGVAARIAAALAGR"
     gene            2209..>2733
                     /locus_tag="P5164_18790"
     CDS             2209..>2733
                     /locus_tag="P5164_18790"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRY45984.1"
                     /translation="MNGPPLPRFVPNPDRPVYGETPRPEWLKVRLVDNEDTRGILDLV
                     RRKHLNTVCQEAQCPNIFECWGRERTATFMLMGDVCTRRCGFCAVTQGIGRPLDPEEP
                     RHVAEAVKELGVRHAVVTSVNRDELPDGGAAHFAATIRAIRTTTPGVTVEVLIPDFLG
                     NDAALRTVLEEAPEV"
BASE COUNT          379 a         1016 c          966 g          372 t
ORIGIN      
        1 gagggcctgg acggcgacgc gcgcgacgtt gttgtagggc tgctgcgccg tgagggagac
       61 ccctgcggtc tgcgtgtgga accggagctt ccacgactcg gggttcttcg cgccgtagcg
      121 gtcgcgcatg accttcgccc agacccggcg cgcagcccgg tacttcgcga tctcctcgaa
      181 gaagtcgttg tgcgcgttga agaagaacga gaggcgcggc gcgaactcgt cgaccggcag
      241 gccggcgtcc acgccgtact tcacgtactc catcccgtcg cggagcgtga aggcgagctc
      301 ctggagagcc gtcgagcccg cctcgcggat gtggtacccc gagatcgaca cggtgttcca
      361 cttcgggacg tgcttcgcgc agaaggcgaa ctggtcggtc acgagccgca tcgaggggcg
      421 cggcgggaag atgaactcct tctgggcgat gtactccttg aggatgtcgt tctggagcgt
      481 cccgccgacc ttcgaccagg gggtcccctg cttctcggcg acggcgaggt acatcgcgag
      541 ggcgatcggc gccgtggagt tgatcgtcat cgacgtcgtc acggagccga ggtcgatccc
      601 gtcgaagagg acctcgaagt cccggagcgt gtcgacggcg acgccgcact tccccacctc
      661 gccggccgac atctcgtgat cggagtcgta gccgtagagg gtcgggaggt ggaaggcggt
      721 cgagaggccc gtctggccgt gcgcgaggag gaccttgaag cgctcgttcg tctgccgggc
      781 ggtgccgaag ccggcgaact ggcggatcgt ccagggcttg ccccggtaca tcgtcgtctg
      841 gacgccgcgg gtgtagggga actcgcccgg gaaggcgagg tcgcgcgcgt agtcgaagcc
      901 ggcgacgtcg tcgggcgtcg cgagcgggtt cacctcggcc gaagagaccg tcgtgaagtc
      961 gcgcttccag gcgggccgtt tcccgaacga ctcggcgacc ctgcggcgcc actcctcctt
     1021 ggcccgggcg atcctctcct catcggcggt cagcggcgtg gcggtcgtct ccgtgctcat
     1081 gcggtcacct cggcgggatc cggatcggcc ctcggggccg cccgaaaaac cggtaactgt
     1141 accagcagcc ccgccgggac gcgcggcacc tcgccccgtc agcgcccggc gagggcggcg
     1201 gcgatccgcg cggcgactcc cgcgttcgag acgacgagcg cctcgttcgc ggccagcgtc
     1261 cgcccgcctg tctcctccgc gagccgcgcc aggaggaacg gggtgacggc ctttccgacg
     1321 accccctccg cgcacgccgc cgcgaggctc cgctcgatcg cgccttccac gacctctcgc
     1381 gggagcgccg ccgcggccgg gacgggattg gcgacgagga tcccgccgct cgacggccag
     1441 gcccaatgcg cggccgcaag gcgggacacc tcggccgcgt cgtcgcaaac gacctcgagc
     1501 ggcaggcccg agccgcgcat ccagaacgcc gggagctccg cggtcccgaa gccgacgacc
     1561 aggacgccga gggtctcgag cgcctcgaga gtcttcggca ggtcgaggac cgccttggcc
     1621 cccgccgcga cgacgacgac gcgggtgcgc gagagctcga ggaggtcggc cgagacgtcg
     1681 aacgtctctt cggcgccgcg gtggactccg ccgatcccgc ccgtcgcgaa gacgcggatg
     1741 ccggcggccg cggcgatcgc catcgtcgcc gcgaccgtcg tcgcgccgct ctccccccgc
     1801 gcggcgagga ggccgaggtc gcgcgtcgtc gccttcctca cgcgcgagcc ggcgcgtgcc
     1861 aatgcctcgg ccccggcgtc gtcgaagccg gcgcgcggga cgccgtcgac gacggcgacg
     1921 agcgccggga ccgccccctc gcgccgcacg gccgcctcgg cggcgcggtg cgtctcgagg
     1981 ctcttcgggt acggcaggcc gtggcagagg atcgtcgact cgagcgcgac gactccgcgc
     2041 ctctccgcga gggccgcctg cacctcgtcc gaaaggacga cgccgccccg ctccgcgctc
     2101 ccgcttcccg ccctcgctcc gctcacgaag ccctccttgc cgggtcccgg cccgccgggc
     2161 tcaccgcgcc tccggcccgt tgccccccga aggctacact ccgcccccgt gaacggaccg
     2221 ccccttcccc gattcgtccc gaaccccgac cggccggtct acggcgagac gccgcgcccc
     2281 gagtggctga aggtccgcct cgtcgacaac gaggacacgc ggggcatcct cgacctcgtc
     2341 cgccggaagc acctcaacac cgtctgccag gaagcgcagt gcccgaacat cttcgagtgc
     2401 tggggccgcg agcggacggc gacgttcatg ctcatgggcg acgtctgcac gcgccgctgc
     2461 ggcttctgcg ccgtgaccca gggaatcggc cggccgctcg acccggaaga gccgcgccac
     2521 gtagccgagg cggtaaagga gctgggcgtc cggcacgcgg tcgtcacctc ggtcaaccgc
     2581 gacgagcttc ccgacggcgg cgcggcccac ttcgccgcga cgatccgcgc gatccgcacg
     2641 acgacgccgg gggtgaccgt cgaggtgctg atccccgact tcctcgggaa cgacgcggcg
     2701 ctccggacgg tcctcgagga agcccccgag gtc
//
LOCUS       DAOTEZ010000521         2726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_29694_length_2726_cov_4.490827, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000521 DAOTEZ010000000
VERSION     DAOTEZ010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29694_length_2726_cov_4.490827"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="P5164_18795"
     CDS             <1..1093
                     /locus_tag="P5164_18795"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRY45985.1"
                     /translation="WEVLDDVVREAGRSLRRLVGTLFSTGDLLAVRRPGSTAMVAFVG
                     LPPGLGEGEAATRLDRKARQVAESVGALLGERFLGRVHRRLQVTAAASRLRYSPQVRL
                     ERLVERALAEASAVASTREGERAAVQKAEFDALLRGGRLEVVFQPIRELASMNVVARE
                     ALTRGPEGSSFESPEVLFEYAARHGRVLSLEQACLSLSATRFVPAGEELLFVNVETHV
                     VNELAHGGLEVLNPLVALGPSVVLEITERGAIADFEAFRGGLSALRRAGFRIALDDAG
                     SGHASLHALAELRPDYLKISQFLVSGLHRDGIKSEIVAMLVRLGERIGAVTVAEGIET
                     EEELAAVRELGVALGQGFLLGRPEAANRR"
     gene            1161..1562
                     /locus_tag="P5164_18800"
     CDS             1161..1562
                     /locus_tag="P5164_18800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45986.1"
                     /translation="MTDHEDGGPELPDGFEKVEATVCRRGGELLVFGTLEPEGPFVFR
                     LQLAKKPEDFGPPPPGFEWTGEERPPKKGEWFWSELRGRAVQAIKDETGVNQFGEPTG
                     GRRILRPAGTAGRPTPTPKHGLRLVRNDEDP"
     gene            complement(1566..1967)
                     /locus_tag="P5164_18805"
     CDS             complement(1566..1967)
                     /locus_tag="P5164_18805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45987.1"
                     /translation="MHAVSPHLAVFRLADVAEVETLVDGEPADIRIEVFEDAANPGQF
                     RCRTWRLVEVGLHPAAPADEDGGLDTEWETLFVPWWVPGGFVTDEPFFAGSKESALEA
                     VLDDLRVYLAVTRHAEDGSEPPAGSPPPASA"
     gene            complement(2003..2079)
                     /locus_tag="P5164_18810"
     tRNA            complement(2003..2079)
                     /locus_tag="P5164_18810"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2043..2045),aa:Arg,seq:cct)
     gene            complement(2126..2356)
                     /locus_tag="P5164_18815"
     CDS             complement(2126..2356)
                     /locus_tag="P5164_18815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY45988.1"
                     /translation="MDRKHLHTFQLVVEGLADPREFLMPFESDDVIQATDACSRYIAR
                     QEDNFLPLGADQAVRANRVLLVKHLSVGPFEE"
     gene            2421..>2726
                     /locus_tag="P5164_18820"
     CDS             2421..>2726
                     /locus_tag="P5164_18820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate-mannose--protein
                     mannosyltransferase"
                     /protein_id="HRY45989.1"
                     /translation="MDRRLLRLALALTLAAAATYALAAFSRDFWAPDEPDFADAVREM
                     NARGSWLLPWQNGKPYSEKPILYYWTMAATTAFTGGEVRPGALRLPSVMAGAFLVLGA
                     "
BASE COUNT          362 a          937 c         1035 g          392 t
ORIGIN      
        1 ctgggaggtc ctcgacgacg tcgtgcgcga ggcggggcgg tcgctccgac ggctcgtcgg
       61 tacgctcttc tcgacggggg acctgctcgc cgtccgccgg ccgggctcga ccgcgatggt
      121 ggcgttcgtc ggcctcccgc ccggacttgg cgagggggag gccgcgacgc ggctcgaccg
      181 gaaggcgcgg caggtggcgg agtcggtcgg ggcgctcctg ggcgagcgct tcctcggacg
      241 cgtccatcgc cgcctgcaag tcacggcggc ggcgtcccgg cttcggtaca gcccgcaagt
      301 gaggctcgag cgcctcgtcg agcgggcgct cgcggaggcc tcggccgtgg cttcgacgcg
      361 ggagggtgaa cgggcggcgg tccagaaggc ggagttcgac gccctcctcc gcgggggccg
      421 gctcgaggtg gtcttccagc cgatccggga gctggcgtcg atgaatgtcg tcgcccggga
      481 ggctctgacg cgcggacccg aagggtcgag cttcgagagc cccgaggtcc tcttcgagta
      541 cgcggcgcgg cacggtcgcg tcctttccct cgagcaggcg tgcctctcgc tctccgcgac
      601 ccgcttcgtc ccggccggcg aggagctcct cttcgtcaac gtggagaccc acgtcgtcaa
      661 cgagctcgcg cacggggggc tcgaggtcct gaatccgctc gtcgccctcg gcccgtccgt
      721 cgtcctcgag atcacggaga ggggcgcgat cgccgacttc gaggcgttcc gcggggggct
      781 ctccgcgctc cggcgagccg gcttccggat cgcgctcgac gacgccggct ccgggcatgc
      841 ctcgctccac gccctggccg agcttcggcc cgactacctg aagatctccc agttcctcgt
      901 ctcgggcctc caccgcgacg ggatcaagag cgagatcgtg gcgatgctcg tccggctcgg
      961 cgagcggatc ggcgccgtga cggtggccga ggggatcgag accgaagagg agctcgccgc
     1021 cgtccgcgag ctcggcgtcg cgctgggcca gggcttcctc ctcgggcggc ccgaagccgc
     1081 gaatcgccgc tgacggaagc ccgcgctcgc tccgagcgcc gtccgggctt tcgggcgatg
     1141 atccgcgcgg ggagacgccg atgacggatc acgaggacgg cgggccggag cttccggacg
     1201 gattcgagaa ggtcgaggcg accgtctgcc gtcgcggggg agagctcctc gtcttcggca
     1261 cgcttgagcc cgagggtccg ttcgtctttc ggctccagct cgcgaagaag cccgaggact
     1321 tcggtccgcc tccccccgga ttcgagtgga ccggagagga gcgtccgccg aagaaggggg
     1381 agtggttctg gtcggagctg cgcgggcgcg cggtgcaggc gatcaaggac gagacgggcg
     1441 tgaaccagtt cggggagccg acgggcggga gacggatcct ccggccggcg ggaacggccg
     1501 gccggccgac gccgaccccg aagcacggcc ttcgcctcgt ccggaacgac gaggacccgt
     1561 aggggtcagg ctgacgcggg cggcggcgag cccgccggcg gctcgctccc gtcctcggcg
     1621 tggcgcgtga cggcgaggta gacgcgaagg tcgtcgagga ccgcctcgag ggcggactcc
     1681 ttcgacccgg cgaagaacgg ctcgtccgtc acgaacccgc cggggaccca ccacggcacg
     1741 aagagcgtct cccactcggt gtcgaggccg ccgtcctcgt cggcgggagc ggcagggtgg
     1801 agcccgacct cgacgaggcg ccaggtccgg caccggaact gcccggggtt cgccgcgtcc
     1861 tcgaagacct cgatccggat gtcggcgggc tctccgtcga cgagcgtctc gacctcggcc
     1921 acgtcggcga ggcggaagac ggcgaggtgg ggagagacgg catgcatcgg gagaggagtc
     1981 tatccggcgc ggcgaagggg agtggtgggc ccggggagac tcgaactccc gacacgcggt
     2041 ttaggaaacc gctgctctat ccacctgagc tacgggcccg tccggagggc ggcgccttcg
     2101 ggaggcggga gcggagtgta ccggatcact cctcgaacgg cccgacggag aggtgcttca
     2161 cgaggaggac gcggttcgcg cggaccgcct ggtccgcgcc gagcgggagg aagttgtcct
     2221 cctgccgcgc gatgtaacgc gagcaggcgt cggtcgcctg gatgacgtcg tcggactcga
     2281 acggcatcag gaactccctc gggtcggcga gcccctcgac gacgagctgg aaggtgtgca
     2341 ggtgctttct gtccatgtgc tcctcggagc cgcggcgaac gggttcgggc ggctcgccga
     2401 cacatcgtat cctccgccgg gtggaccgtc gcctcctccg cctcgcgctc gcgctgacgc
     2461 tcgccgccgc cgcgacgtac gccctcgcgg ccttctcgcg cgacttctgg gcccccgacg
     2521 agcccgactt cgccgacgcc gtccgggaga tgaacgcgcg gggctcgtgg ctgctgccct
     2581 ggcagaacgg gaagccctac tcggagaagc cgatcctcta ctactggacg atggcggcga
     2641 cgacggcctt caccggggga gaggtccgtc cgggggctct ccggctcccg tccgtgatgg
     2701 ccggggcgtt cctcgtcctc ggggcg
//
LOCUS       DAOTEZ010000522         2717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_29840_length_2717_cov_7.257701, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000522 DAOTEZ010000000
VERSION     DAOTEZ010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29840_length_2717_cov_7.257701"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1279
                     /locus_tag="P5164_18825"
     CDS             164..1279
                     /locus_tag="P5164_18825"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004042.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HRY45990.1"
                     /translation="MRETPAPPTRSASRRSGAAALLAAGLALAAGFAACGPSSSLAGP
                     PAGKQTKPEKVEKMKTVLSKSGHDITPLSREEVARLAKKLDPESYRVTQKSGTEAPFC
                     GNLLDNKKEGTYLCVVCGLPLFSSEHKFDSGTGWPSFFREFDPAHVTRVTDTSHGMKR
                     VEINCARCGAHLGHVFEDGPAPTGERHCLNSASLTFLENGAPVPPESRPVKAETAYFA
                     AGCFWGVQHWFSRGPGVLEATSGYMQGRTEKPTYRQVCEGDTGHAETVKVVYDANRIT
                     YRRLVEAFFSMHDPTQLNRQGPDVGDQYRSGIWTVDAGQEKVAREILEGLAARKEYGE
                     KRIVTLVEPAKTFWPAEEYHQEYLAKNGAACHVTNPW"
     gene            1357..1914
                     /locus_tag="P5164_18830"
     CDS             1357..1914
                     /locus_tag="P5164_18830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRY45991.1"
                     /translation="MSENEARERLAKLMVAYQGGDESAFESLYGELAGPVRAFLASLT
                     RNAVRADDLLQETFFHVHRARQTYDPARSAKAWIYAIAHNVFLMACRTDRRRGRNEEL
                     PTDELPDLPVPAEAESLASRDLVSRALATLPVDRREALLLHHVEGLSFQEVGAVLGVT
                     TMAAKLRSHRAMGQLRDLLAREGAR"
     gene            1911..2624
                     /locus_tag="P5164_18835"
     CDS             1911..2624
                     /locus_tag="P5164_18835"
                     /inference="COORDINATES: protein motif:HMM:NF018264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HRY45992.1"
                     /translation="MSGTPLPPALRAAVASDLRPVRPLASPARRALTLLAWAAVAATL
                     VLALRGLRSDVAELGVLLTWGVVLAEVAAGAGLVALALAEAVPARGPGRGAALLALGG
                     AAALFVALAAATRRASRGMEVDDPLLSFGPSCAGLQGVIGLTAFAVAALLVLRAAPLR
                     ARLAGLLAGAGTGFLAEGVYHLDCPITHLSHVLVWHGSAILLLAVFGFGFGLVLEARE
                     RARMERRRAGTAARKGGEE"
BASE COUNT          374 a         1031 c          943 g          369 t
ORIGIN      
        1 cgtttcggcc ggccgcgcgg gccggtccgc gggtctctgc acccgggatg ccgccccgcc
       61 ggaaaggttt cagccggcgg aaacgaatcg atcgacggtc tccggaccga tccattcgcg
      121 aaaccggagc gtacctgcga gcatcacgga gagcgtgacg acgatgcgcg agacccccgc
      181 acccccgacc cgatccgcct cccgccgttc gggagccgcc gccctcctcg ccgccggcct
      241 cgcgctcgcg gccggcttcg ccgcctgcgg cccctcgagc tcgctcgcgg ggcctccggc
      301 cggaaagcaa acgaaacccg agaaggtgga aaagatgaag acggtcctct cgaagtccgg
      361 ccacgacatc acgcccctct cccgcgagga ggtcgcccgc ctcgcgaaga agctcgaccc
      421 ggagtcgtac cgggtgacgc agaagtccgg gacggaagcc ccgttctgcg ggaacctcct
      481 cgacaacaag aaggagggga cgtacctctg cgtcgtctgc ggcctccccc tcttctcgag
      541 cgagcacaag ttcgactcgg gaacgggctg gccgagcttc ttccgtgagt tcgacccggc
      601 tcacgtgact cgtgtgaccg acacgtctca cggcatgaaa cgggtcgaga tcaactgcgc
      661 ccgctgcggc gcccacctcg ggcacgtgtt cgaggacgga ccggccccga cgggcgagcg
      721 ccactgcctg aactccgcct cgctgacgtt cctcgagaac ggagcgcccg tgccgccgga
      781 gagccgcccc gtgaaggccg agaccgccta cttcgccgcc ggctgcttct ggggggtcca
      841 gcactggttc tcgcgcgggc cgggcgtcct cgaggcgacg agcggctaca tgcagggacg
      901 gacggagaag ccgacctacc ggcaggtctg tgagggagac actgggcacg ccgagacggt
      961 gaaggtcgtc tacgacgcga accgcatcac gtaccgtcgg ctcgtcgagg cgttcttctc
     1021 catgcacgac ccgacgcagc tgaaccgcca ggggcccgac gtcggcgacc agtaccgctc
     1081 ggggatctgg accgtcgacg ccgggcagga gaaggtggcg cgggagatcc tcgaggggct
     1141 cgccgcgcgg aaagagtacg gcgagaagag gatcgtgacg ctcgtcgagc cggcgaagac
     1201 gttctggccc gccgaggagt accaccagga gtacctcgcg aagaacggcg ccgcctgtca
     1261 cgtgacgaac ccgtggtgat cggcgagtga gcggcgcgtg accgcgagcg agccgcgatt
     1321 tccccggggg cggtccgggc ggctacgatc cgcgcgttgt ccgagaacga ggcacgggag
     1381 aggctggcga agctgatggt ggcgtaccag ggcggggacg agtccgcgtt cgagagcctc
     1441 tacggcgagc tcgcgggccc cgtgagggcg ttcctcgcct cgctgacgcg gaacgcggtc
     1501 cgcgcggacg acctcctcca ggagacgttc ttccacgtcc accgcgcgcg gcagacgtac
     1561 gacccggcgc gctccgcgaa ggcgtggatc tacgcgatcg cgcacaacgt cttcctgatg
     1621 gcctgccgca cggaccgaag gcgcggccgg aacgaggagc tcccgacgga cgagctgccc
     1681 gacctccccg tcccggccga ggccgagtcg ctcgcttcga gggacctcgt ctcgcgcgcc
     1741 ctcgcgacgc tccccgtcga ccgccgcgag gcgctcctcc tccaccacgt ggaggggctc
     1801 tcttttcagg aggtgggggc cgtcctcggc gtcacgacga tggccgccaa gctccgctcg
     1861 caccgcgcca tgggacagct ccgggacctc ctcgcgcggg aaggagcgcg atgagcggca
     1921 caccgctccc gcccgcgctt cgcgccgccg tcgcgagcga cctccggccg gttcgcccgc
     1981 tcgcctcgcc cgctcggcgc gccctcaccc tcctcgcctg ggccgccgtc gccgcgacgc
     2041 tcgtcctcgc cctccggggc ctccgctccg acgtcgccga gctcggcgtc ctcctgacgt
     2101 ggggcgtcgt cctcgccgag gtcgccgcag gcgccgggct cgtcgcgctc gccctcgccg
     2161 aggccgttcc cgcgcggggg ccggggcgcg gcgccgcgct cctcgccctc ggcggcgccg
     2221 cggccctctt cgtcgcgctc gccgccgcga cgcgccgcgc gagccgcggg atggaggtcg
     2281 acgacccgct cctgagcttc ggcccctcct gcgccggcct tcagggcgtc atcggcctga
     2341 cggccttcgc cgtcgccgcc ctcctcgtcc tccgggcggc gccgctccgc gcccggctcg
     2401 cggggctcct cgccggcgcc gggacgggtt tcctcgccga gggggtctac cacctcgact
     2461 gccccatcac gcacctctcg cacgtcctcg tctggcacgg ctccgccata ctcctcctcg
     2521 cggtcttcgg cttcggcttc ggcctcgtcc tcgaggcgag ggagcgggcg cggatggaac
     2581 gacggcgcgc cggcacggcc gcgcggaaag gaggcgagga atgagcgacg aggtcttccg
     2641 gaagctcacg agcctcagct cctgcgcggg gtgagcgtcg aagctgcccc ccgggcagct
     2701 cgcgcaggtc ctgcgcg
//
LOCUS       DAOTEZ010000523         2714 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_29903_length_2714_cov_5.362166, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000523 DAOTEZ010000000
VERSION     DAOTEZ010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29903_length_2714_cov_5.362166"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="P5164_18840"
     CDS             <1..1093
                     /locus_tag="P5164_18840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRY45993.1"
                     /translation="ILLHVRTTKGKGYALAEKDPVFWHGPSPFKVETGEVAKKAAPPS
                     YTAVFADTLADLARKDERVVAITAAMPDGTGLDRFAKEFPARTFDVGICEQHAVLFAA
                     GMATQGMKPVCAIYSTFLQRAYDQVFHDVCLMDLPVVFALDRGGVVGADGPTHHGAYD
                     LSYLRVFPNMHVAAPKDENELRGLVATALALGRPVAIRYPRGAGHGVALDAEPKLLPV
                     GKGEVLRVGRDGVVVAIGDPVLPALGAAERLAAEGGASLSVLNARWVKPLDTELLAQF
                     VKPGTTVFTVEENAVAGGFGSAVSEALDALGLAARVHRLGIPDRFVPHATQAEQRAEL
                     GIDEEGLLRAFRTGTAGAGVVPLRSRVAS"
     gene            1107..2123
                     /locus_tag="P5164_18845"
     CDS             1107..2123
                     /locus_tag="P5164_18845"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRY45994.1"
                     /translation="MRALVCERPGHVALRDLPEPVPGPGEVVVRVESALTCGTDLKLI
                     RRGHPKVPFPTVLGHEYCGRVVGTGAGVSFTVGERVVSGVSGPCGECADCREGRPNHC
                     PTAFDHAVWGTWAEQVRIPARVVASTLRRVPEALSSDAAALLDPLASVLRGLGRLPDP
                     SGRTVLVLGSGPIALMFTALLLRRGAARVVVAGRNAGRLAAHASLGADTISARESLGP
                     QVRDRSGSRGAELVVETTGAPEVAEEAPGLAARGGTVLLFAGLAREARLAVLAHRVHY
                     DEVTLAGSFHYTPEDARTALDLLARGELPVDLLVTSESPLEKHPFVLERLSRGEEMKV
                     AFRP"
     gene            2120..>2714
                     /locus_tag="P5164_18850"
     CDS             2120..>2714
                     /locus_tag="P5164_18850"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRY45995.1"
                     /translation="MIPSAMKAALVRAPGQVEIVEMETPRPGPGEILVAMKAVVVCGS
                     DVHPWYVASKAPAVLGHETAGVVAETGPGVVHVRSGDRVFVHHHVPCGACRSCEAGEP
                     VMCPEWKPSRLHPGGLAEYVRVEAPSVARDALLLPPHVSFTGGAFVEPVACALKAVDR
                     ALVRPGDAALVVGLGSNGLLLGWLARLAGAALLVGSDP"
BASE COUNT          313 a         1034 c          990 g          377 t
ORIGIN      
        1 gatcctcctc cacgtccgga cgacgaaggg gaaggggtac gccctcgccg agaaggaccc
       61 ggtcttctgg cacgggcctt cgccgttcaa ggtcgagacg ggcgaggtcg cgaagaaggc
      121 cgcgcctccg agctacacgg ccgtcttcgc cgacacgctc gccgacctgg cccggaagga
      181 cgagagggtc gtcgcgatca cggcggcgat gcccgacggc accggcctcg accgcttcgc
      241 gaaggagttc ccggcgcgca cgttcgacgt cgggatctgc gagcagcacg ccgtcctctt
      301 cgcggccggg atggcgacgc aggggatgaa gccggtctgc gccatctact cgacgttcct
      361 ccagcgcgcc tacgaccagg tcttccacga cgtctgcctc atggaccttc cggtcgtctt
      421 cgcgctcgac cggggcgggg tcgtcggcgc ggacgggccg acgcaccacg gcgcctatga
      481 cctctcctac ctccgcgtct tcccgaacat gcacgtcgcc gcgccgaagg acgagaacga
      541 gctgcggggc ctcgtcgcca cggcgctcgc cctcggccgg ccggtcgcca tccgctaccc
      601 gcgcggcgcc gggcacggcg tggcgctcga cgccgagccg aagctcctgc cggtcggaaa
      661 gggcgaggtc ctccgcgtcg gccgggacgg cgtcgtcgtg gcgatcggcg acccggtcct
      721 gccggcgctc ggcgcggccg agcggctcgc ggccgagggg ggggcgtccc tctccgtcct
      781 gaacgcccgc tgggtgaagc cgctcgacac cgagctcctc gcccagttcg tgaagcccgg
      841 gacgaccgtc ttcaccgtcg aggagaacgc cgtcgccggc ggcttcggct ccgcggtttc
      901 cgaggcgctc gacgccctcg ggctcgcggc ccgcgtccac cggctcggga tcccggaccg
      961 cttcgtcccg cacgccacgc aggccgagca gcgggcggag ctcgggatcg acgaggaggg
     1021 gctcctccgc gccttccgga cggggaccgc cggagcgggc gtcgtcccgc tccggtcccg
     1081 ggtcgcgagc tgacgtcacg aggacgatgc gcgcgctcgt ctgcgagcgg cccgggcacg
     1141 tcgcccttcg ggacctcccc gagcccgtgc cgggaccggg agaggtcgtc gtccgggtgg
     1201 aatcggcgct gacctgcgga accgacctga agctgattcg acgcggtcat ccgaaggtgc
     1261 cgttcccgac ggtcctcggg cacgagtact gcggacgcgt cgtgggcacc ggcgcggggg
     1321 tctcgttcac ggtcggcgag cgcgtggtct cgggcgtgag cggaccgtgc ggggagtgcg
     1381 cggactgccg cgaaggccgc ccgaaccact gtccgaccgc cttcgatcac gcggtctggg
     1441 ggacctgggc cgagcaggtc cggatccccg cgcgcgtcgt cgcctcgacg ctccggcgcg
     1501 tccccgaggc gctttcgtcc gacgccgcgg cgctcctcga cccgctggcc tccgtcctgc
     1561 gcggcctcgg ccggctgccc gacccgtcgg ggcggacggt cctcgtcctc gggtcgggac
     1621 cgatcgccct gatgttcacg gccctgctcc tgcgccgcgg ggcggcccgc gtcgtcgtcg
     1681 ccggacggaa cgccggccgg ctcgcggcgc atgcctcgct cggcgccgac acgatctccg
     1741 cgcgggagag cctcggcccg caggtccgcg accggagcgg ctcgcgcggg gccgagctcg
     1801 tcgtcgagac gaccggcgcg cccgaggtgg ccgaggaggc cccgggcctc gccgcacgcg
     1861 gcgggacggt cctcctcttc gccggcctcg cccgggaggc gcgcctggcg gtcctggccc
     1921 accgcgtcca ctacgacgag gtgacgctgg ccggatcgtt ccactacacg ccggaggacg
     1981 ctcggacggc cctcgacctc ctcgcccggg gagagcttcc ggtcgacctc ctcgtcacgt
     2041 cggaaagccc cctggagaag cacccgttcg tgctcgagcg gctctcgcgc ggcgaggaga
     2101 tgaaagtggc gttccggccg tgatcccgtc cgcgatgaag gccgcgctcg tccgtgctcc
     2161 cgggcaggtc gagatcgtcg agatggagac cccgcggccc gggccgggag agatcctcgt
     2221 cgccatgaag gcggtcgtcg tctgcgggag cgacgtccat ccctggtacg tcgcctcgaa
     2281 ggccccggcc gtcctggggc acgagaccgc gggagtcgtg gccgagacgg gacccggcgt
     2341 cgtccacgtc cggagcggcg accgggtctt cgtccaccac cacgtcccgt gcggcgcctg
     2401 ccgctcctgc gaggcaggag agccggtcat gtgtccggag tggaagccgt cgcgcctcca
     2461 tcccgggggg ctcgccgaat acgtccgcgt cgaggcgccc tcggtcgccc gcgacgcgct
     2521 cctccttccg ccgcacgtct cgttcaccgg gggcgcgttc gtcgagccgg tcgcgtgcgc
     2581 gctcaaggcg gtggaccgcg ctctcgtccg gcccggggac gcggcgctcg tcgtcggcct
     2641 cggctccaac ggcctcctcc tcggctggct cgcacggctc gccggcgccg cgctcctcgt
     2701 cgggagcgac cccg
//
LOCUS       DAOTEZ010000524         2708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_30022_length_2708_cov_5.797965, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000524 DAOTEZ010000000
VERSION     DAOTEZ010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30022_length_2708_cov_5.797965"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /gene="hslV"
                     /locus_tag="P5164_18855"
     CDS             complement(<1..360)
                     /gene="hslV"
                     /locus_tag="P5164_18855"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HRY45996.1"
                     /translation="MSDTPAFRHTTVLAVRKGGSVALGSDGQVTLGTTVLKHGASKIR
                     RLAGGSVLAGFAGSTADAFALFTRFERKLEEFSGQLERAAIELATDWRTEKTLRALEA
                     LLVVADREKTLLLSGTGD"
     gene            complement(583..1497)
                     /gene="xerC"
                     /locus_tag="P5164_18860"
     CDS             complement(583..1497)
                     /gene="xerC"
                     /locus_tag="P5164_18860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HRY45997.1"
                     /translation="MSLAEALGAWDRHLADERAVSPHTRKAYVDDVGRFLEFLSAFSG
                     EEPGSIEPGDVDALAVRSWLASLRSGGQARTSIGRRLSALRTFFTFLQREGLAEGNPA
                     RGVATPRTDKNLPVTLSVAEAAAVVEAPASDTELGLRDRALLELLYATGLRVSELVGL
                     SLPDVDLPGRQVRTLGKGRKERIVPFGEKAAEALAAWLPARLALLRGRRVRGEPLFLN
                     ARGGRLTDRSVRRVLDQALSAAEVARHASPHALRHSFATHLLSAGADLRTIQELLGHA
                     SLATTQKYTHLDVERLMEVYRKAHPKAE"
     gene            complement(1494..2699)
                     /gene="trmFO"
                     /locus_tag="P5164_18865"
     CDS             complement(1494..2699)
                     /gene="trmFO"
                     /locus_tag="P5164_18865"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HRY45998.1"
                     /translation="MVCSNSFRSDNPGNAVGLLKREMELVGSLVMEEARRAAVPAGDA
                     LAVDRELFARAVTARVEADPAIELVREEAPALPDPGDGFVVVATGPLTSEALAGSLLA
                     RLGGDRLYFYDAVAPIVEGSSLDLSALYAASRWGKGSGDDYLNVPLTREAYEAFVAAL
                     LAGEKVPYHDFEKAVHFEGCLPIEEMAARGPETLRHGPMKPFGLRDPRTGREPYAAVQ
                     LRQDDLAKSHWNLVGFQTKLKVGEQRRVFRTLPGFANAEFVRYGMLHRNTFLNGPAHL
                     DRFFRFKGDPRVLFAGQVTGVEGYLESAATGLMVGATLAQLLEGREPLPLPFSTALGA
                     LSRHVSESDPEHYGPMNVTFGLIDDSALPPIRDRAKRREEIVRRALAEVAAWRDAALG
                     PAPEASGAA"
BASE COUNT          369 a          941 c         1038 g          360 t
ORIGIN      
        1 gtcgcccgtc ccggagagga ggagcgtctt ttcccggtcg gcgacgacga ggagcgcttc
       61 gagggcccgg agggtcttct cggttcgcca gtcggtcgcg agctcgatgg cggctcgctc
      121 gagctgaccc gagaactctt ccagctttcg ctcgaaccgg gtgaagaggg cgaaggcgtc
      181 agccgtcgac ccggcgaagc cggcgaggac ggatccgccg gcgaggcggc ggatcttgga
      241 ggcgccgtgc ttcaagaccg tcgtcccgag cgtcacctgc ccgtcgctcc cgagggcgac
      301 ggagcccccc ttgcggacgg cgaggacggt cgtatggcgg aaggcgggcg tgtcgctcac
      361 ggcggggagt ctacccgccg ctcactcccg gcccttcctc gttgtacacc cgcctgcgac
      421 tcgtgatcgc ggaaagcgcc cgggggccat cggtcggctg tcatgttctc ggaggaccgc
      481 cgagcggcct ggccgcgagg tgaaggcgga gccaacccgg acgccgggtt gatttcgtct
      541 tcgcttcgcg gtccccgaac ccctccgcgc gggcttctcc tcttactcgg ccttcgggtg
      601 ggccttccgg tagacctcca tgagccgctc gacgtcgagg tgggtgtact tctgggtcgt
      661 cgcgagcgag gcgtggccga ggagctcctg gatcgtccgc aggtcggcgc cggccgagag
      721 gaggtgggtc gcgaaggagt gccgaagggc gtggggcgag gcgtgacggg cgacctcggc
      781 ggccgagagc gcctggtcga ggacgcggcg gacgctccgg tcggtgagcc gccccccccg
      841 ggcgttcagg aagagcggct cgccccggac cctccggccg cggaggagcg cgaggcgcgc
      901 cgggagccag gccgcgagcg cctccgccgc cttctcgccg aacgggacga tccgctcctt
      961 gcgtcccttg ccgagcgtcc ggacctggcg gccgggaagg tcgacgtcgg ggagcgagag
     1021 gccgacgagc tccgacacgc ggagccccgt cgcgtagagg agctcgagga gcgcccggtc
     1081 acgcaggccg agctccgtgt cggaggccgg cgcctcgacg acggccgccg cctccgcgac
     1141 ggagagcgtg acggggaggt tcttgtcggt ccgcggggtc gcgacgccgc gcgccgggtt
     1201 cccctcggcg agcccctccc gctggaggaa cgtgaagaag gtccggagcg ccgagaggcg
     1261 tctcccgatc gaggtccgcg cctgcccccc ggagcggagc gaggcgagcc aggagcggac
     1321 ggcgagcgcg tccacatctc ccggctcgat cgagccgggc tcctcgccgg aaaacgcgga
     1381 gaggaactcg aggaagcggc cgacgtcgtc cacgtaggcc ttccgtgtgt ggggcgagac
     1441 ggcccgctcg tcggcgaggt ggcggtccca ggcgccgagg gcctcggcga ggctcacgcc
     1501 gcccccgacg cctccggcgc gggcccgagc gccgcgtcgc gccaggcggc gacctcggcg
     1561 agcgcccggc ggacgatctc ctcgcgccgc ttcgcccggt cgcggatcgg cgggagcgcg
     1621 gagtcgtcga tgaggccgaa cgtgacgttc atcgggccgt agtgctcggg gtcgctctcg
     1681 gagacgtgcc gggagagggc gccgagcgcc gtcgagaagg ggagcgggag cggctcgcgc
     1741 ccctcgagga gctgcgcgag cgtcgccccg accatcaggc cggtcgccgc cgactcgagg
     1801 tatccctcga cgcccgtcac ctgcccggcg aagaggacgc gcgggtctcc cttgaagcgg
     1861 aagaagcggt cgaggtgcgc cgggccgttc aggaacgtgt tgcgatggag catcccgtag
     1921 cggacgaact cggcgttcgc gaagccggga agggtccgga agacgcgccg ctgctccccg
     1981 accttcagct tcgtctggaa gccgacgagg ttccagtggc tcttcgcgag gtcgtcctgg
     2041 cggagctgga ccgccgcgta cggctcgcgg ccggtgcgcg ggtcgcgcag tccgaacggc
     2101 ttcatcggcc cgtgccggag cgtctccggg ccgcgcgcag ccatctcctc gatcggaagg
     2161 cacccctcga aatggaccgc cttctcgaag tcgtggtacg gcaccttctc gccggcgagg
     2221 agcgcggcga cgaacgcctc gtacgcctcg cgcgtcagcg ggacgttcag gtagtcgtcc
     2281 ccgctcccct ttccccagcg gctcgcggcg tagagggcgg agaggtcgag cgacgagccc
     2341 tcgacgatcg gggcgacggc gtcgtagaag tagagccggt ctccgccgag ccgcgcgagg
     2401 agagagccgg ccagggcttc ggaggtgagc ggcccggtcg cgacgacgac gaaaccgtcg
     2461 cccgggtcgg gaagggccgg cgcctcctcg cggacgagct cgatcgccgg atcggcctcg
     2521 acgcgcgcgg tgacggcccg ggcgaacagc tcccggtcga ccgcgagggc gtcaccggcc
     2581 ggcacggccg cgcgacgcgc ctcttccatc acgagcgacc cgacgagctc catctcgcgt
     2641 ttcaggaggc cgacggcgtt ccccgggttg tccgaccgga aggagttcga gcagaccatc
     2701 tcggcgag
//
LOCUS       DAOTEZ010000525         2708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_30026_length_2708_cov_5.327931, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000525 DAOTEZ010000000
VERSION     DAOTEZ010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30026_length_2708_cov_5.327931"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="P5164_18870"
     CDS             <1..624
                     /locus_tag="P5164_18870"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY45999.1"
                     /translation="FPGTVVLVVLFAAIFSTISVIEDRREGFLQAVVVSPASPLSVAA
                     GKIAGGTLLALAQGGVLLALAPLLGFAPSVVALAPALLVLALVALALTGLGLAIAWML
                     ESTQGFHAIMNLVLIPMWFLSGSFFPAEGAPAWLRAAMAVNPLTYGVASLRRALDPAT
                     SAGPSATLSVAVTLAFALGAALAAAAVIARFPAGPDHPARPSRRKQP"
     gene            621..1265
                     /locus_tag="P5164_18875"
     CDS             621..1265
                     /locus_tag="P5164_18875"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRY46000.1"
                     /translation="MSPEIPRRRVSPALAVVLVLLLLAAVAGGLYLLLHRPTAGPDLP
                     RLGGAPAFALVSESGKTVTAAEYAGTVWIADFIFTRCGGSCPILSSRMLALSRKTADV
                     PGIRYVSFGVDPEHDTPEVLAAYARDLGADLSRWSFLHGGRPVIRTLIKDGFKLAIED
                     GPADSVEPILHSTRFVLVDGEGTIRGYYDGMEQPPVDVLERDARALAAALPERR"
     gene            1262..1681
                     /locus_tag="P5164_18880"
     CDS             1262..1681
                     /locus_tag="P5164_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HRY46001.1"
                     /translation="MTFSDLPTVNAILNGAAASLLVAGFVLIRAGRREAHRRAMTAAF
                     ACSVLFLVSYLVYHFEAGSVRFQGTGPVRTFYLTVLLTHTVLAAVVPFLAVTTLVLAR
                     KERFEAHRRLARVTLPIWLYVSVTGVVIYLMLYRMGF"
     gene            1739..2581
                     /gene="prmC"
                     /locus_tag="P5164_18885"
     CDS             1739..2581
                     /gene="prmC"
                     /locus_tag="P5164_18885"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRY46002.1"
                     /translation="MPTWGQLLAEGRSLLDSPAFAPFEAEELLASAAGRPRGWFHARR
                     RDEAHDGDAARYRDLVARRAAGEPLQYLLGEWEFLGRTFRVDPRALIPRGETEAIVEI
                     AREAAPAARSILDGGTGSGVLAVSLAIERPEARVVAVDRSEAALALARANAALHGVAA
                     RVSFLASDWLSALAPRPLFDLAVANPPYVPRVDAPHVEKTVSDHEPHLALFGGDDGLD
                     AVRVLLAELPPRLLPGAPFLFEIGYGQAREVCGLVEASPAFTLEDVRLDPAGIPRTVV
                     ARRR"
BASE COUNT          273 a         1167 c          842 g          426 t
ORIGIN      
        1 ttcccgggca ccgtcgtcct cgtcgtcctc ttcgccgcga tcttctcgac gatctccgtc
       61 atcgaggacc ggcgagaagg gtttctccag gccgtcgtcg tctcccccgc ttcgccgctt
      121 tccgtcgcgg ccggaaagat cgccggggga acgctcctcg ccctcgcgca ggggggcgtc
      181 ctcctcgcgc tcgcgccgct cctcggcttc gctccctcgg tcgtcgcgct cgcgccggcc
      241 ctcctcgtcc tcgcgctcgt cgccctcgcg ctcaccggcc tcggcctcgc gatcgcgtgg
      301 atgctcgagt cgacgcaggg gttccacgcc atcatgaacc tcgtcctgat cccgatgtgg
      361 ttcctctccg gctccttctt ccccgccgag ggggcgcccg cgtggctccg cgccgcgatg
      421 gccgtgaacc cgctcaccta cggcgtcgcc tcgctccggc gcgcgctcga cccggccact
      481 tcagccggtc cgtccgcgac gctctccgtc gccgtcacgc tcgccttcgc cctcggcgcc
      541 gccctcgcgg ccgccgccgt catcgcgcgc ttcccggccg gccccgacca tcccgcgcgc
      601 ccttcccgga ggaagcagcc gtgagccccg agatcccccg ccgccgcgtt tcgcccgccc
      661 tcgccgtcgt cctcgtcctc ctgctcctcg cggcggtcgc cggagggctc tacctcctcc
      721 tccatcgccc gaccgccggg cccgacctcc cgcgcctcgg cggcgccccc gccttcgcgc
      781 tcgtctccga gagcgggaag acggtgacgg ccgcggagta cgccggtacc gtctggatcg
      841 ccgacttcat cttcacgcgc tgcggcggca gctgcccgat cctctcctcg cgaatgctcg
      901 ccctctcgag gaagacggcc gacgtccccg ggatccggta cgtctcattc ggcgtcgacc
      961 ccgagcacga cacgccggag gtcctcgccg catacgcgcg cgacctcggc gccgacctct
     1021 cccgctggag cttcctccac ggcggccggc cggtgatccg cacgctgatc aaggacggct
     1081 tcaagctcgc gatcgaggac ggccccgccg acagcgtcga gccgatcctg cactccaccc
     1141 gcttcgtcct cgtcgacggc gaagggacga tccgcggcta ctacgacggg atggagcagc
     1201 ctcccgtcga cgtcctcgag cgggacgccc gggccctcgc cgccgcgctc ccggagcgcc
     1261 gttgaccttc tccgacctgc ccaccgtcaa cgcgatcctg aacggagccg cggcgtcgct
     1321 cctcgtcgcc ggcttcgtcc tgatccgcgc gggccggcgc gaggcgcacc ggcgggcgat
     1381 gaccgccgcc ttcgcctgtt ccgtcctctt cctcgtctcc tacctcgtct accacttcga
     1441 ggcgggcagc gtccgcttcc aggggacggg ccccgtccgg accttctacc tgacggtcct
     1501 cctgacgcac accgtcctcg ccgccgtcgt cccgttcctc gccgtgacga ccctcgtcct
     1561 cgcgcggaag gaacgcttcg aggcgcaccg gcgcctcgcg cgcgtgacgc tcccgatctg
     1621 gctctacgtc tccgtcaccg gcgtcgtcat ctacctgatg ctctaccgca tgggcttctg
     1681 aggcggacgc gcggcgcccg cgcgagaggt cctgccctcg tggtggcaag atccacccgt
     1741 gccgacctgg ggccagctcc tcgccgaggg acgatccctc ctcgactctc ccgcgttcgc
     1801 cccgttcgag gccgaggagc tcctcgcctc ggccgccggg cgtccgcgcg gctggttcca
     1861 cgcgcggcgg cgcgacgagg cccacgacgg cgacgcggcc cgctaccgcg acctcgtcgc
     1921 ccgacgcgcc gcgggagagc cgctccagta cctcctcggc gagtgggagt tcctgggacg
     1981 gacgttccgc gtcgacccgc gcgccctgat cccgcgcggc gagaccgagg cgatcgtcga
     2041 gatcgcgcgc gaggcggccc ccgcggcccg ctcgatcctc gacggaggga cggggagcgg
     2101 cgtcctcgcc gtctcgctcg cgatcgagcg gccggaggcg cgcgtcgtcg cggtagaccg
     2161 gtccgaggcg gccctcgcgc tcgcgcgggc gaacgccgcg ctccacggcg tcgccgcgcg
     2221 cgtctccttc ctcgcctccg actggctctc ggccctcgcg ccgcgccccc tcttcgacct
     2281 cgcggtcgcg aaccccccgt acgtcccgcg cgtcgacgcg ccccacgtcg agaagaccgt
     2341 ctcggaccac gagccgcacc tcgccctctt cggcggcgac gacggcctcg atgccgtccg
     2401 cgtcctcctg gcagagctcc cgccgcgcct cctcccgggc gcgccgttcc tcttcgagat
     2461 cggctacggc caggcgcgcg aggtgtgcgg cctcgtcgaa gcgagccccg cgttcacgct
     2521 cgaggacgtc cgcctcgacc cggccggcat cccgcgcacc gtcgtcgccc gccgccgctg
     2581 aggggccacg gcggccccgg gccgcgtact acactccgcc cgctttggac gccttcctcg
     2641 tctccggccc ctcccgcctc tcgggcactc tcgccgtctc cggcgcgaag aacgccgccc
     2701 ttcccgac
//
LOCUS       DAOTEZ010000526         2705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_30087_length_2705_cov_7.007925, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000526 DAOTEZ010000000
VERSION     DAOTEZ010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30087_length_2705_cov_7.007925"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="P5164_18890"
     CDS             complement(<1..730)
                     /locus_tag="P5164_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HRY46003.1"
                     /translation="MDVLTLEVGSTITKANGFVRFPGGGFDHVAQGFAPTSVVQGDVG
                     IGVDAALADLRARSGLDPAGAETFVNSSAAGGLRMTVHGLTMSMTARAAREAALGAGG
                     IVKLVTAGELESWELDEIRAISPNLVLLAGGVDHGEKAIVLKNAEKLAALIADGLRVP
                     VLYAGNAALRRPVTELFRLAGVELHVAENVFPEVDVLNVGPLRRLVHEAFNRHIVNAP
                     GMHRLAELTRHEVLPTPGAVLRGAE"
     gene            complement(819..>2705)
                     /gene="oraE"
                     /locus_tag="P5164_18895"
     CDS             complement(819..>2705)
                     /gene="oraE"
                     /locus_tag="P5164_18895"
                     /EC_number="5.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002515917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine 4,5-aminomutase subunit OraE"
                     /protein_id="HRY46004.1"
                     /translation="IRTTGQSHIDGLLEGTPEGIGGVPITRKQIRASRKACDEIEKEV
                     GRPINFHSYVSGVAGPEVAVLFAEEGINGAHQDPQYNVLYRNVNMYRSFVDAAEAKKV
                     MAGARIFQIDGAHNANATAKAGWLVMPELMVQHGINCAFSVAVGMEKDLIGLSTVPPN
                     APPAPKLWYDLPYAVALRDFFSDYRMRAQQNTRYIEADIEEAVRTHTVDTLISMLTRA
                     DIQSTITPDEGRNVPWHYNNVRGVQTVKQTWAALDGIKELVSLNHDGPLGEMVRDLKE
                     RAVAFLEEVLEVGGYFAAVEKGFFVDSAKYPERSGDGIARDGKGGVGAGSLVPRDADY
                     LAPVCDHFGTNHLPASAAKACDPIGGCTLCDPSKIVFIDELDPEDNCHRRLEKTLPYR
                     KGTAIRPEAEWAGDGTVLVQLFVPENEEIAPVYAVEMAKKMGLVDPEVINTMVLHPAE
                     GCFVEVKGKVAFDVDRTTLKIPEKEVILPEQELRDAVKKVGLKVVAGTVGEDEHSVGL
                     REILDIKHGGIEKYGVKYHYLGTSVPVGKLVDAAVETGAHAILISTIISHNDVHRSQM
                     RKLAELCREKGIRDRVVLVAGGTQVNRDMAKETGLDATFGRGTKGIHVVNAMVKALRA
                     RGTA"
BASE COUNT          353 a          933 c          961 g          458 t
ORIGIN      
        1 gctcggcgcc gcggaggacg gctcccgggg tcgggaggac ttcgtggcgc gtcagctccg
       61 cgaggcggtg catgcccggc gcgttcacga tgtggcggtt gaaggcctcg tggacgaggc
      121 ggcggagcgg gccgacgttc aggacgtcga cctcggggaa gacgttctcg gcgacgtgga
      181 gctcgacgcc ggccaggcgg aagagctcgg tgaccgggcg ccggagcgcc gcgttcccgg
      241 cgtagaggac cgggacgcgg agaccgtccg cgatcagcgc cgccagcttc tcggcgttct
      301 tcaggacgat ggccttctcg ccgtggtcta cccctccggc gaggaggacg aggttcgggg
      361 agatcgcgcg gatctcgtcg agctcccagc tctcgagctc gcccgccgtc accagcttca
      421 cgatcccgcc ggcgccgagc gccgcctcgc gcgcggcgcg ggccgtcatc gacatcgtca
      481 ggccgtggac ggtcatccgg aggcccccgg ccgccgagct gttgacgaac gtctcggcgc
      541 cggcggggtc gaggccggac cgggcccgga ggtccgcgag cgccgcgtcg acaccgatcc
      601 cgacgtcgcc ctgcacgacg ctcgtcgggg cgaagccctg ggcgacgtgg tcgaagcctc
      661 cgcccgggaa gcggacgaag ccgttcgcct tcgtgatcgt cgagccgacc tcgagcgtca
      721 ggacgtcgat cttcatctcc ggcccagggc cgcgcctccc gatcggtgga tcgcgggacg
      781 cggccgggaa acggccgcgc cccgccgctg cgttccgctc aggccgttcc gcgcgcccgg
      841 agcgccttca ccatcgcgtt gacgacgtgg atccccttcg tgccgcgtcc gaacgtcgcg
      901 tcgaggccgg tctccttcgc catgtcacgg ttcacctggg tgccgccggc gacgagcacg
      961 acgcggtcgc ggatcccctt ctcccggcag agctcggcga gcttccgcat ctgggaacgg
     1021 tggacgtcgt tgtggctgat gatcgtggag atcaggatgg cgtgcgcccc ggtctcgacg
     1081 gcggcgtcga cgagcttgcc gacggggacc gacgtcccga ggtagtgata cttcacgccg
     1141 tacttctcga tgccgccgtg cttgatgtcg aggatctcgc gaaggccgac gctgtgctcg
     1201 tcctcgccga cggtcccggc gacgaccttg agaccgacct tcttcacggc gtcccgaagc
     1261 tcctgctcgg ggaggatcac ttccttctcg gggatcttca gcgtcgtccg gtcgacgtcg
     1321 aacgcgacct tccccttcac ctcgacgaag cacccctccg ccgggtggag gaccatcgtg
     1381 ttgatgacct ccgggtcgac gaggcccatc ttcttcgcca tctcgacggc gtagaccggg
     1441 gcgatctcct cgttctcggg gacgaagagc tggacgagga ccgtcccgtc gcccgcccac
     1501 tcggcctcgg ggcggatcgc cgtccccttc cggtagggca gcgtcttctc gagccgccgg
     1561 tggcagttgt cctccgggtc gagctcgtcg atgaagacga tcttcgaagg gtcgcagagc
     1621 gtgcagccgc cgatcgggtc gcaggccttc gccgcgctcg cggggaggtg gttcgtcccg
     1681 aagtggtcgc agacgggggc caggtagtcc gcgtcgcgcg ggacgagcga gccggctccg
     1741 acgcccccct tcccgtcgcg ggcgatgccg tcgccgctcc gctcggggta cttcgccgag
     1801 tcgacgaaaa agcccttctc gaccgccgcg aagtagccgc ccacctcgag gacctcctcg
     1861 aggaaggcga cggcgcgctc cttcaggtcg cgcaccatct cgccgagcgg cccgtcgtgg
     1921 ttgagcgaga cgagctcctt gatcccgtcg agggcggccc aggtctgctt gaccgtctgg
     1981 acgccgcgga cgttgttgta gtgccacggc acgttccgcc cctcgtcggg ggtgatcgtg
     2041 ctctggatgt cggcgcgggt gagcatcgag atgagggtgt cgaccgtgtg ggtcctcacc
     2101 gcctcctcga tgtcggcctc gatgtagcgg gtgttctgct gcgcccgcat ccggtagtcg
     2161 ctgaagaagt cgcggagggc gaccgcgtac ggcaggtcgt accagagctt cggagcgggc
     2221 ggggcgttcg gcgggacggt ggagaggccg atcaggtcct tctccatccc gacggcgacc
     2281 gagaaggcgc agttgatccc gtgctggacc atcagctcgg gcatgacgag ccagcccgcc
     2341 ttggccgtgg cattggcgtt gtgggcgccg tcgatctgga agatccgcgc tccggccatc
     2401 accttcttcg cctccgccgc gtcgacgaag gagcggtaca tgttcacgtt ccggtagagg
     2461 acgttgtact gcgggtcctg gtgggcgccg ttgatcccct cctcggcgaa gaggacggcg
     2521 acctcggggc cggcgacgcc ggagacgtac gagtggaagt tgatcgggcg gccgacctcc
     2581 ttctcgatct cgtcgcacgc cttgcggctc gcccggatct gcttgcgcgt gatcgggacg
     2641 ccgccgatcc cctcgggcgt cccctcgagg aggccgtcga tgtggctctg ccccgtcgtc
     2701 cggat
//
LOCUS       DAOTEZ010000527         2687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_30416_length_2687_cov_6.801672, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000527 DAOTEZ010000000
VERSION     DAOTEZ010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30416_length_2687_cov_6.801672"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="P5164_18900"
     CDS             complement(<1..662)
                     /locus_tag="P5164_18900"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46005.1"
                     /translation="MSRPAAPRAAQSFPGAILVALSLLAPTTAQASAWSPVGPPGGAA
                     YGLALDPKDANVLYAGAFGGGVWKSTDGGARWGRLSGLPAEETVNAVAVHPTDSRTVL
                     AAGFTALWRSADGGSTWKKVLDQKVQQPTMTGFAFDPTAPATVYASADNDGFPTGVFK
                     STDSGATWKAANVGINPNSRVRGVAVSRDGKSVFAASEDGVYLSTDGGASWTVALPRK
                     AA"
     gene            complement(978..1508)
                     /locus_tag="P5164_18905"
     CDS             complement(978..1508)
                     /locus_tag="P5164_18905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46006.1"
                     /translation="MDAARVIALLRPLLEATGTRWMLAGGLALVAWGSTRTTADTDLL
                     VDDAFRSALLARLGAAGFEVLLDSDGFTNLLHSEREIGRLDLIWVDGETSRRLFAAAV
                     ERTGPDAIPLLVPAPEHLVAMKVKAMKEQPTRVFRDAEDLRLLLSLPDLDEDSVRATF
                     ERAGLGELHERIKSAS"
     gene            complement(1591..2061)
                     /locus_tag="P5164_18910"
     CDS             complement(1591..2061)
                     /locus_tag="P5164_18910"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRY46007.1"
                     /translation="MTATARSFRVELDVRFPEVDSYRVVWHGNYVLYGEVARNALCAA
                     AGLTPAEALARGYRVPITRFDVAVRRPARLDDRLEVSCTLRSPETAKLEMDYEVRRLP
                     DRLLLATAFTQQVVLSPQDELLLTFPKLLKELVERILAYQRGERELTGEKIPVG"
     gene            complement(2058..>2687)
                     /locus_tag="P5164_18915"
     CDS             complement(2058..>2687)
                     /locus_tag="P5164_18915"
                     /inference="COORDINATES: protein motif:HMM:NF014819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46008.1"
                     /translation="QKDPAGLNALFYARFLLEDGRARAIVAGAVDEWNLTYHLSYERL
                     GVLATATRAGYAMTEGCSVVFLETAESARARGARPWARLAGAASSGSPVSPQARRARP
                     GDVAATLRAALGDAGVEPAAVGLVHASANGVPWVDAAEAAALADVFGTSPLPRVERVK
                     LLAGENPCSGALQLALAARALRDDPSLGAVVVNSLGAGGNQIAAVFTAP"
BASE COUNT          381 a          895 c         1080 g          331 t
ORIGIN      
        1 tgcgcggcct tcctcgggag ggcgaccgtc caggacgccc cgccgtccgt ggagaggtag
       61 acaccgtcct cggaagcggc gaagacggac ttcccgtccc gcgagacggc gacgccgcga
      121 acgcgcgagt tcgggttgat cccgacgttc gccgccttcc acgtggcgcc ggagtcggtc
      181 gacttgaaga cgcccgtcgg gaagccgtcg ttgtcggcgc tcgcgtagac ggtcgccggc
      241 gcggtcggat cgaacgcgaa gccggtcatc gtgggctgct gcaccttctg gtcgaggacc
      301 ttcttccagg tcgacccgcc gtcggccgag cgccagaggg cggtgaagcc ggctgcaagg
      361 accgtgcggg agtcggtcgg gtggaccgcg acggcattca cggtctcctc tgcgggaaga
      421 cccgagaggc ggccccagcg cgcccctccg tcggtcgact tccagacgcc gccgccgaac
      481 gcccccgcgt agaggacgtt cgcgtccttc gggtcgagcg cgagcccgta ggctgccccg
      541 cccggtgggc cgacggggct ccaggcggaa gcctgtgccg tggtgggcgc gagcagggag
      601 agagcgacga ggatcgctcc ggggaagctc tgcgcggctc tcggggcggc gggtcggctc
      661 atgcggcctc cttgcggcag tgccgcgggg gaggtctctc cccgcgggcg tacggttacc
      721 ggacgaagga cgcggagagt acgtggcctg gatgcggagg ggaaggggcc cgtgcgcgga
      781 cgtgtcgggc ggctcagccg tcgagtgtga accgggggcc ccgcgcgagg gggatcgccc
      841 ggagctgctc cgcggtccat cggaactgcc tcaggaagcg ggcgtactcc ggcagcgaca
      901 tcggcccggg gcgatgcctc tcgagcgatt cggcgacggc gggagacgcc gacggaagcg
      961 cgtcgagatc gaggggctca ggacgcgctc ttgatccgct catgaagctc gccgaggccg
     1021 gcccgctcga acgtcgcgcg gacggaatcc tcgtccagat cgggaaggga gaggagcagc
     1081 cgaaggtcct cggcgtcgcg gaacacccgg gtcggctgct ccttcatcgc cttgaccttc
     1141 atggcgacga ggtgctccgg ggcagggacg aggaggggga tcgcgtccgg gccggtcctc
     1201 tcgacggcgg cggcgaacag ccggcggctc gtctcgccgt cgacccagat caggtcgagc
     1261 cggccgatct cgcgctcgga gtgcaggagg ttcgtgaaac cgtcggagtc caggaggacc
     1321 tcgaatcccg ctgctccgag gcgcgcgagg agggcggagc ggaaggcgtc gtcgacgagg
     1381 aggtccgtgt cggccgtcgt gcgtgtggag ccccaggcca cgagggccag gccccccgcg
     1441 agcatccacc gcgtcccggt cgcctcgagg aggggcctca ggagcgcaat aacgcgtgcc
     1501 gcgtccatcc gggcggactc tacgccgccg gagcgtcgcc gggctaccgg cgccggacct
     1561 cggggaggac gagaagtccc cccgcgggac ctacccgacc gggatcttct cccccgtaag
     1621 ctccctctcc ccgcgctggt acgcgaggat ccgctcgacg agctccttca ggagcttcgg
     1681 gaacgtcagc aggagctcgt cctgcgggga gaggacgacc tgctgcgtga aggccgtggc
     1741 gaggaggagc cggtcgggaa ggcggcggac ctcgtagtcc atctcgagct tcgccgtctc
     1801 gggcgagcgg agcgtgcagg agacctcgag gcggtcgtcg aggcgcgcgg ggcggcgaac
     1861 ggccacgtcg aaccgcgtga tcggcacccg gtagccgcgg gcgagcgcct cggctggcgt
     1921 gaggcccgcc gcggcgcaga gggcgttgcg cgccacctcg ccgtacagga cgtagttgcc
     1981 gtgccagacg acgcgatagg agtcgacctc cgggaaccgg acgtcgagct cgacacggaa
     2041 ggagcgcgcg gtcgccgtca cggcgcggtg aagacggcgg cgatctggtt gccgccggcc
     2101 ccgagagagt tgacgacgac ggccccgagc gacgggtcgt cgcggagggc gcgcgcggcg
     2161 agggcgagct ggagggcgcc ggagcagggg ttctcgcccg cgaggagctt gaccctctcg
     2221 acgcgcggca gcggcgaggt tccgaagacg tcggcgaggg ccgccgcctc ggcggcgtcg
     2281 acccagggga cgccgttcgc ggaggcgtgg acgagtccga cggccgccgg ctcgaccccg
     2341 gcgtcgccaa gggcggcgcg gagggtcgcg gcgacgtcgc cggggcgggc gcggcgcgcc
     2401 tgaggcgaga cgggagagcc ggaggaggcg gcgccggcga gccgcgccca ggggcgggcg
     2461 ccgcgcgcgc gggccgactc cgcggtctcg aggaagacga cggagcaccc ttcggtcatc
     2521 gcgtagcccg cccgggtggc ggtcgcgagg acgccgaggc gctcgtacga gaggtggtag
     2581 gtcaggttcc actcgtcgac cgcgccggcg acgatcgccc gcgcccggcc gtcctcgagg
     2641 aggaagcggg cgtagaagag ggcgttcagg ccggccgggt ccttctg
//
LOCUS       DAOTEZ010000528         2687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_30417_length_2687_cov_6.647036, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000528 DAOTEZ010000000
VERSION     DAOTEZ010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30417_length_2687_cov_6.647036"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1417
                     /locus_tag="P5164_18920"
     CDS             <1..1417
                     /locus_tag="P5164_18920"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HRY46009.1"
                     /translation="TGMRRRGEEAREGSRPSPGRGALLPRLGWLLAALLFLAFVAGLV
                     RRGRIEQAGEQVIRMLFVPSVEQGTLARRGNELAEFVRKDTGLVIRSAVPTSYAAVVQ
                     ALGVGQADIAWVPAFAYVVANARFGAEARLQVVREAERYAVVVTRNGPGEPAKLGDLA
                     GKRIAIPKSFSPELRATLVPELDKWSPGWREVPAEDDREAVRQLLDRADVVDAAASSW
                     VFSGPKDLVGDGRKLLEYERPGTLEKTRVAFKTAEPARERVTVYYGSVMTRSDSGVSR
                     LEDLAGRPFAFSDATSTSGYIFPLNLLNATGVKLGHVYYAGGHANVVQAVADGKVAGG
                     SSFYSPPGKVNEVERTFVADARHLLMKRIPTDEGRLAFLDEVRVLALTDPIPNDVCCV
                     RRGFPKATWDRFERSLRKFIATPEGLRAYLDLVASVDARSCDDGTFDGFRATLRSTGV
                     SAVQLLEAEEEKLRKKREGKK"
     gene            1414..2193
                     /gene="phnC"
                     /locus_tag="P5164_18925"
     CDS             1414..2193
                     /gene="phnC"
                     /locus_tag="P5164_18925"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014322876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HRY46010.1"
                     /translation="MTPPVAVAVERLSKVWPDGIKALDDVSLEVREGEFLAVLGLSGS
                     GKSTLLRCINRLVDPTEGKISIFGRDVTGAEGADLRALRREVGMIFQQFNLVKRHTVR
                     DNVLSGALGRTGTLRSLLLLFPEADRAEAQACLERVGMGDRGDSRADALSGGQQQRVA
                     IARALMQRPKLILADEPVASLDPALRNSVMRHVEALNREEGITVICSLHDLDLMKRYA
                     TRAVALREGKLVAEGKPEEFDLPTLRSIYGEEAEFGFEAAS"
     gene            2190..>2687
                     /locus_tag="P5164_18930"
     CDS             2190..>2687
                     /locus_tag="P5164_18930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46011.1"
                     /translation="MRRAAPPPPGPGARLLALVVDAALLATFLLMGELLVRALVFPMQ
                     EGTLRTAQEILEKLHYAVLGGAGLLLALAWNGAAGLVGGTPGQAFAGALPQEPAERPW
                     DRTLRGWFTLLFVELTFFTGWLVTEVDLGTFFTNFDKASGVLRGLLHPSGAVLFEGLI
                     LLAQTL"
BASE COUNT          359 a          985 c          951 g          392 t
ORIGIN      
        1 gaccgggatg aggaggcgag gagaagaggc ccgggagggg tcccgtccgt cgccgggtcg
       61 cggagcgctc ctcccgaggc tcggctggct cctcgccgct ctcctcttcc tcgcgttcgt
      121 cgcgggcctc gtgagacgcg gccggatcga gcaggccggg gagcaggtca tccggatgct
      181 cttcgtcccc tccgtcgagc agggaacgct cgcgcggcgc gggaacgagc tcgcggagtt
      241 cgtgaggaag gacaccggcc tcgtcatccg ctccgccgtc ccgacgagct acgccgcggt
      301 cgtccaggcg ctcggcgtcg ggcaggccga catcgcctgg gtccccgcct tcgcctacgt
      361 cgtcgcgaac gcccgcttcg gcgccgaggc gcggctccag gtcgtccgcg aggccgagcg
      421 atacgcggtc gtggtgacgc gcaacggccc cggcgagccg gccaagctcg gcgacctcgc
      481 cgggaagagg atcgcgatcc cgaagagctt ctcgcccgag cttcgcgcca cgctcgtccc
      541 cgagctcgac aagtggtcgc cgggctggag agaggtgccg gcggaggacg accgcgaggc
      601 cgtccggcag ctcctcgacc gggccgacgt cgtcgacgcg gcggcctcga gctgggtctt
      661 ctccggcccg aaggacctcg tcggcgacgg gaggaagctc ctcgaatacg agcggcccgg
      721 gaccctcgag aagacgcgcg tcgcgttcaa gacggccgag cccgcccgag agcgggtgac
      781 ggtctactac ggctcggtga tgacccgctc cgactcgggc gtctcgcgcc tcgaggacct
      841 cgccggccgg ccgttcgcgt tctccgacgc gacctccacc tccggctaca tcttccctct
      901 caacctcctg aacgcaacgg gcgtgaagct cgggcacgtc tactacgccg gcgggcacgc
      961 gaacgtcgtc caggcggtcg cggacgggaa ggtcgccggc ggctcctcgt tctactcgcc
     1021 ccccgggaag gtgaacgagg tggagcggac gttcgtcgcc gacgcgcggc acctcctgat
     1081 gaagcggatc ccgaccgacg aggggcgcct cgcgttcctc gacgaggtcc gcgtcctcgc
     1141 tctgacagac ccgatcccga acgacgtctg ctgcgtgcgg cgcggcttcc cgaaggcgac
     1201 gtgggaccgc ttcgagcgat cgctccggaa gttcatcgcg acgcccgaag ggcttcgcgc
     1261 ctacctcgac ctcgtcgcct cggtcgacgc ccgctcctgc gacgacggga cgttcgacgg
     1321 cttccgcgcg acgctccggt cgaccggcgt cagcgccgtc cagctcctcg aggccgagga
     1381 ggagaagctc cggaagaagc gggaggggaa gaagtgaccc cgcccgtcgc cgtcgcggtc
     1441 gagcgcctgt cgaaggtctg gcccgacggc atcaaggccc tcgacgacgt gtcgctcgag
     1501 gtccgcgagg gggagttcct ggccgtcctc ggactctccg gctcggggaa gtcgacgctc
     1561 ctgcgctgca tcaatcgcct ggtcgacccg acggagggga agatctcgat cttcggccgc
     1621 gacgtgaccg gcgccgaggg ggccgacctg cgcgcgctgc gccgcgaggt cgggatgatc
     1681 ttccagcagt tcaacctcgt gaagcggcac acggtgcggg acaacgtcct ttcgggggcg
     1741 ctcgggagga ccggcacgct ccggagcctc ctcctcctct tcccggaggc cgatcgggcc
     1801 gaggcgcagg cgtgcctcga gcgggtcggg atgggggatc ggggcgacag ccgcgccgac
     1861 gccctctcgg gcgggcagca gcagagggtc gccatcgccc gcgcgctgat gcagcggccg
     1921 aagctgatcc tcgccgacga gccggtcgcg tcgctcgacc cggccctccg gaactccgtc
     1981 atgcggcacg tcgaggcgct gaaccgggag gaggggatca ccgtgatctg ctccctccac
     2041 gacctcgacc tgatgaagcg ctacgcaacg cgcgccgtcg cgctccgcga ggggaagctc
     2101 gtcgccgagg ggaagcccga ggagttcgac ctcccgaccc tccggtcgat ctacggcgaa
     2161 gaggccgagt tcggcttcga ggcggcctcg tgaggcgcgc cgctccgcct ccgcccgggc
     2221 cgggcgcccg gctcctcgcg ctcgtcgtcg acgccgcgct cctcgcgacg ttcctcctga
     2281 tgggcgagct cctcgttcgc gccctcgtct tcccgatgca ggaggggacc ctccggaccg
     2341 cccaggagat cctcgagaag ctccactacg cggtcctcgg cggggcgggg ctcctcctgg
     2401 ccctggcctg gaacggggcc gccggactcg tcggcgggac gcccggccag gccttcgccg
     2461 gggcgctccc gcaggagccg gccgagcggc cgtgggaccg aacgcttcgt ggctggttca
     2521 cgctcctctt cgtcgagctg acgttcttca cgggctggct cgtcaccgag gtcgacctcg
     2581 gcacgttctt cacgaacttc gacaaggcct cgggcgtcct gcgcggtctc ctccacccgt
     2641 cgggcgcggt cctcttcgag gggctgatcc tcctcgccca gacgctc
//
LOCUS       DAOTEZ010000529         2682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_30503_length_2682_cov_5.855348, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000529 DAOTEZ010000000
VERSION     DAOTEZ010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30503_length_2682_cov_5.855348"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="P5164_18935"
     CDS             complement(<1..749)
                     /locus_tag="P5164_18935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RND family transporter"
                     /protein_id="HRY46012.1"
                     /translation="MTKERITRFLESMVFGHRPVVLGLFAVATLFMAWSASHLRVDAG
                     FQKNLPHAHPYMETFLKHQQEFGGANRVLVAMMAREGDIFTAEFLDALKKATDEVFFI
                     PGVDRSRVQSLFTPNVRYTEVVEDGIAAGNVIPDDFEPNAEGLAQVRRNVLKAGIVGR
                     LVANDFSGTIISAELLEVDPSTGQKLDFIAVSNALEEKVRRQFDAEFVPGSKVDVRII
                     GFAKVVGAISAGTRRVILFFLVAYALTGLLV"
     gene            complement(806..1765)
                     /locus_tag="P5164_18940"
     CDS             complement(806..1765)
                     /locus_tag="P5164_18940"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HRY46013.1"
                     /translation="MREKSLPAFGAALLALATLAPAGAAEPPAAPERSMPARLAARSL
                     LLDAADREGLAVAVGERGHVLVSRDGGASWTQAEAPTRALLTGVWLHDARLGWAVGHD
                     ETILRTRDGGATWQRVRHAPEEEKPLLDVWFRDERQGFAIGAYGLLLATGDGGDTWES
                     RTVVEGHDTHLNAIAEAGGSLFLAGEAGSLYRSDDGGTSWQALPSPYEGSFFGLLPLS
                     DGGLLAFGLRGHLFRSEDRGRTWTGVASGTEATLMAGRELGGGKVVLAGLAGVLLHSE
                     DGGRTFRARELGDRKASLAILPGAGGGIVLLGEGGVRTIEKPF"
     gene            complement(1782..>2682)
                     /locus_tag="P5164_18945"
     CDS             complement(1782..>2682)
                     /locus_tag="P5164_18945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HRY46014.1"
                     /translation="GIPFPIPKSGIEVIWNHLLRYRAEIAGRTVAQAAPTRGGQYTLV
                     QFEEETMFVYHLPGTTEASMKNRLLNFKQAVTAPARLAGGILLVHESMNQVAQPRDAW
                     LYNPGQRRVRRAPQVAYDNPGTAADNMRTSDQLDMFNGAPDKYDWTLVGKKEIYVPYN
                     AYRLQDPKVKYKDILTPLHMNPDHTRYELHRVWVVDATLKKGERHIYKRRTFYIDEDS
                     WQILVVDQYDNRDQLWRVSEGHAIAYYDLPTIWTSAESHTDLQAGRYLVMGLNSENAP
                     HDFKIKRTEGDFTPDALRREGVR"
BASE COUNT          377 a          897 c          988 g          420 t
ORIGIN      
        1 tagacgagga ggcccgtgag ggcgtaggcg acgaggaaga agaggatgac gcggcgggtg
       61 ccggcggaga tggccccgac gaccttcgcg aagccgatga tcctcacgtc caccttcgag
      121 cccgggacga actccgcgtc gaactgtcgg cggaccttct cctcgagggc gttcgagacg
      181 gcgatgaaat cgagcttctg accggtcgaa gggtcgacct cgaggagctc ggcgctgatg
      241 atcgtccccg agaagtcgtt cgccacgagc cggccgacga tcccggcctt caggacgttg
      301 cggcggacct gcgcgagccc ctcggcgttc ggctcgaagt cgtccgggat gacgttgccg
      361 gcggcgatcc cgtcctcgac gacctcggtg tagcggacgt tcggcgtgaa gagcgactgg
      421 acgcggctcc ggtcgacgcc gggaatgaag aagacctcgt cggtcgcctt cttcagcgcg
      481 tcgaggaact cggcggtgaa gatgtccccc tcgcgcgcca tcatcgccac gagcacgcgg
      541 ttcgcccctc cgaactcctg ctggtgcttc aggaaggtct ccatgtaggg gtgggcgtgg
      601 gggaggttct tctggaaccc ggcgtcgacc ctgaggtgcg acgcggacca ggccatgaag
      661 agcgtcgcca cggcgaacag cccgaggacg accggccgat ggccgaagac catggattcc
      721 agaaagcgcg tgatccgttc cttcgtcatc ttctttccct cacccctccg ccggcgtccc
      781 gggcgcgagc ccgggagcgc ccgccctaga acggcttctc gatcgtccgg accccaccct
      841 cgccgaggag gacgatcccg cccccggccc ccgggaggat cgccagggag gccttccggt
      901 cgcccagctc ccgagcccgg aacgtgcggc ctccgtcttc gctgtggagg aggacgcccg
      961 ccaggcccgc gaggacgacc ttcccgccgc cgagctcccg gccggccatc agcgtcgctt
     1021 cggtgccgga agcgacgccc gtccaggtcc ggccccgatc ctcgctgcgg aagaggtgcc
     1081 cccgaagccc gaaggcgagg aggcccccgt cagagagagg aagcaggccg aagaacgagc
     1141 cctcgtaggg cgaggggagc gcctgccagc tcgtgccgcc gtcgtcggag cggtagaggg
     1201 agcccgcctc ccccgcgagg aagagcgacc ctcccgcctc cgcgatggca ttgaggtggg
     1261 tgtcgtgacc ctcgacgacc gtccggctct cccacgtgtc gccgccgtcc ccggtcgcga
     1321 ggaggaggcc gtaggcgccg atcgcaaagc cctgtctttc gtcgcggaac cagacgtcga
     1381 ggagcggctt ctcctcctcg ggggcgtggc ggacgcgctg ccaggtcgcc ccgccgtcgc
     1441 gcgtccggag gatggtctcg tcgtgcccca cggcccaccc gaggcgggcg tcgtgcagcc
     1501 agacgccggt cagcagggcc cgcgtcggcg cctcggcctg ggtccacgac gctccgccgt
     1561 cgcgggagac gaggacgtgc ccgcgctcgc cgaccgcgac ggcgaggccc tcgcggtcgg
     1621 cggcgtccag gaggagcgaa cgggcagcga gccgggcggg catcgaccgc tcgggcgcgg
     1681 cgggaggctc cgccgcgccc gctggggcca gtgtcgcgag ggcgagcagg gccgcgccga
     1741 acgcgggaag ggacttctca cgcatggcgc ggccctccgg gtcagcggac gccttcgcgc
     1801 cggagagcgt ccggcgtgaa gtcgccctcg gtccgcttga tcttgaagtc gtgcggtgcg
     1861 ttctcgctgt tcaggcccat gacgaggtag cgccccgcct ggaggtcggt gtgggactcg
     1921 gcggaggtcc agatcgtcgg caggtcgtag taggcgatgg cgtgcccttc cgagacgcgc
     1981 cagagctggt cgcggttgtc gtactggtcg acgacgagga tctgccagct gtcctcgtcg
     2041 atgtagaacg tccggcgctt gtagatgtgc ctctcgccct tcttgagggt cgcgtcgacg
     2101 acccagaccc ggtgcagctc gtagcgggtg tggtcggggt tcatgtggag gggcgtgagg
     2161 atgtccttgt acttgacctt cgggtcctgg agccggtagg cgttgtaggg gacgtagatc
     2221 tccttcttgc cgacgagcgt ccagtcgtac ttgtccggcg cgccgttgaa catgtcgagc
     2281 tggtcgctcg tccgcatgtt gtcggcggcc gtgcccgggt tgtcgtaggc cacctgcggg
     2341 gcgcggcgga cgcggcgctg gccggggttg tagagccagg cgtcgcgcgg ctgggcgacc
     2401 tggttcatcg actcgtgaac gaggaggatg ccgcccgcga ggcgcgccgg ggccgtgacc
     2461 gcctgcttga agttgaggag gcggttcttc atcgaggcct cggtcgtgcc ggggaggtgg
     2521 tagacgaaca ttgtctcctc ctcgaactgc acgagcgtgt actgcccgcc gcgggtcggg
     2581 gccgcctgcg cgaccgtgcg gccggcgatc tccgcccggt accggaggag gtggttccag
     2641 atgacctcga tgccgctctt cgggatcggg aacgggatgc cg
//
LOCUS       DAOTEZ010000530         2681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_30514_length_2681_cov_6.081493, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000530 DAOTEZ010000000
VERSION     DAOTEZ010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30514_length_2681_cov_6.081493"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            398..>2681
                     /locus_tag="P5164_18950"
     CDS             398..>2681
                     /locus_tag="P5164_18950"
                     /inference="COORDINATES: protein motif:HMM:NF024295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HRY46015.1"
                     /translation="MPPRFCTSRRPWFVVGLLVMAASGLLASPAFAQLPVYTDSLQSG
                     FADWSWGTRNLAQTAVVHGGTAAISFEPDGWAGLFLHRDAGFSGAEYSAIELWVRGAG
                     SGGQQVTVAILSGGSPAGSAPLAGFVAGGSIPAGSWAKATVPFSALGLPASGSWDGLW
                     LQDATGGNQATLYVDDVRLLPNDSPPPSGGPVTVSVDPALDRRTVSPHIFGVNFGTAA
                     QAAQLRWPARRWGGNSVTRYNWQNDTSNKGMDWFFMNVPEDNPDPSTLPSGSASDRFL
                     DETRAAGGEPILTVPLIGWTPKARQKDWGFSVAKYGAQTQTECTATGGAFWCQPDAGN
                     GVRAAGGNVTGNDPLDTSIAIGPSFVTAWMDHLAARYGTAGNGGIRWYALDNEPALWN
                     STHRDVHPEPLTYDELWTKTRDVAAAIKAKDPAAVVFGPVSWGWCEYFYSAADDCSPN
                     GADHVAHGRVPLTQWYLRQVRQYEEQFGVRLVDVLDVHYYPQASGVALSDDESAATSA
                     RRLRSLRSLWDPSYVDESWIPDAVRLIPRMKEWIAAELPGAKLAITEYSWGNDDGISS
                     ALAQVEALGIFAREGVDVATRWVAPAAGSRVEDAFRLWMNYDGAGARVTGASVRAASG
                     APDAVSGYAVEAAGGLLRVVLVNRDTVQRPVTVSLAGDGSRGVRLFGFDGTNRLAALG
                     STAFGSGQLTLDVPARAARLLEVAPEGGTASIGTPVITAPTAGQVLDVAGVNLSWNAV
                     TNATGYDLRLLSSTSGATVFS"
BASE COUNT          380 a          947 c          963 g          391 t
ORIGIN      
        1 acgacgattg cctgttgtct cgggcggatt ccgggaaagc ttcacatcct ctgagcgggg
       61 cccgcgctcg gaaggaaagc gacccacaga cacactccgc gcaggagaaa gcgggtggag
      121 atccccacgt gcttcatctc attcctcccc ggcggtcctt gtccaagggc cgggccgcat
      181 ttccgtggac ttgggaagtc tattcctcgc ggagcgacca gacctcccaa actcgaggaa
      241 tcacaggcat gagaagccgt cccaagtgcg gtctcgccgg gaggccacgg ccaggccggg
      301 gagagggcga acctcgacct gaggcgagtc gactccgcgg cgctcgcggc ggtacggttg
      361 tctccaacag atgtacgcga ttcgaacgag acccccgatg cccccgcggt tctgcacttc
      421 tcgacggccc tggttcgtcg tcggcctcct cgtgatggcg gcgtccggtc tcctcgcctc
      481 acccgccttc gcgcagctcc ccgtctacac cgactcgctc cagagcggct ttgccgactg
      541 gtcctggggg acgcggaacc tcgcgcagac ggccgtcgtc cacggcggga cggccgcaat
      601 ctctttcgag ccggacggct gggccgggct cttcctccac cgggacgccg gcttcagcgg
      661 cgcggagtac tccgcgatcg agctctgggt gcgtggggcg ggctccggcg ggcaacaggt
      721 gacggtggcg attctgagcg gcgggagccc ggccggctcc gcgccgctcg ccggattcgt
      781 ggcggggggc tcgatccctg ccgggagctg ggcgaaggcg acggtgccgt tctccgcgct
      841 cggcctgccg gcctccggga gctgggacgg tctttggctg caggacgcca cgggcgggaa
      901 ccaggcgacg ctctacgtcg acgacgtccg ccttctgccg aacgactcac cgcccccttc
      961 gggcgggccg gtgaccgtct ccgtcgaccc ggcgctcgac cgccggacgg tcagcccgca
     1021 catcttcggc gtgaacttcg gaacggcggc tcaggcggct cagctgcggt ggcccgcgcg
     1081 gcgctggggc ggcaactcgg tgacccgcta caactggcag aacgacacct cgaacaaggg
     1141 gatggactgg ttcttcatga acgtccccga ggacaacccc gacccgtcga ctcttccgtc
     1201 cggatcggcc tccgaccgtt tcctcgacga gacgcgcgcg gcggggggcg agccgatcct
     1261 taccgtgccg ctcatcggct ggacgccgaa ggcgcggcag aaggactggg gcttctcggt
     1321 cgcgaagtac ggcgcgcaga cgcagaccga atgcacggcg acgggcggcg cgttctggtg
     1381 ccaacccgac gcgggaaacg gagtgcgcgc cgcgggcggg aacgtgaccg ggaacgaccc
     1441 gctcgacacc tccatcgcga tcgggccgtc gttcgtgacg gcctggatgg accacctcgc
     1501 cgcgcggtac gggacggcgg ggaacggcgg catccgctgg tacgcgctcg acaacgagcc
     1561 ggcgctctgg aactcgacgc atcgcgacgt ccatcccgag ccgctgacgt acgacgagct
     1621 ctggacgaag acgcgcgacg tcgccgcggc gatcaaggcg aaggacccgg ccgcggtcgt
     1681 cttcgggccg gtctcgtggg gctggtgcga gtacttctac tccgcggccg acgactgctc
     1741 gccgaacggc gcggaccacg tcgctcacgg tcgcgtgccg ctcacgcagt ggtacctgcg
     1801 gcaggtgcgg cagtacgagg agcagttcgg cgtccgcctc gtcgacgtcc tcgacgtcca
     1861 ctactacccg caggcctcgg gcgtggccct ctcggacgac gaatccgcgg cgacgtcggc
     1921 gcggcggctc cgctcgctcc ggagcctctg ggacccgtcc tacgtcgacg agtcgtggat
     1981 ccccgacgcg gtccgcctca tcccgcggat gaaggagtgg atcgccgcgg agcttcccgg
     2041 ggcgaagctc gcgatcacgg agtacagctg ggggaacgac gacgggatct cgagcgcgct
     2101 cgcgcaggtg gaggcgctcg gcatcttcgc ccgcgagggg gtcgacgtgg cgacgcgctg
     2161 ggtcgcgccc gccgccgggt cgcgcgtcga ggacgcgttc cggctttgga tgaactacga
     2221 cggcgccggc gcgcgcgtca ccggggcgag cgtccgcgcc gcctccggtg ccccggacgc
     2281 cgtgagcggc tacgccgtgg aagcggccgg agggctcctc cgcgtcgtcc tcgtgaaccg
     2341 cgacacggtg cagcggccgg tgacggtctc gctcgccggc gacgggtcgc ggggcgtccg
     2401 cctctttggc ttcgacggca cgaaccgcct cgccgcgctc ggcagcaccg ccttcggcag
     2461 cggtcagctg acgctcgacg tgccggcacg cgcggcgcgg ctcctggagg tcgccccgga
     2521 aggaggcacg gcgtcgatcg ggacgcccgt gatcacggcg ccgacggcag gacaggtgct
     2581 ggacgtggcg ggcgtgaacc tctcgtggaa cgccgtgacg aacgctacgg gctacgacct
     2641 gcgcctcctc tcctcgacga gcggggcgac cgtcttcagc g
//
LOCUS       DAOTEZ010000531         2665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_30807_length_2665_cov_3.993870, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000531 DAOTEZ010000000
VERSION     DAOTEZ010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30807_length_2665_cov_3.993870"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2214
                     /locus_tag="P5164_18955"
     CDS             <1..2214
                     /locus_tag="P5164_18955"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HRY46016.1"
                     /translation="NQSYPLTVGEDGFITIPDGGGRVYTNGVSLKEFRGMVLRSLSQA
                     YSTYINAQDPAQSTAFVDVKLGKLRRLFVFVVGEVRKQGALSVSPGAATVLNLLNNAG
                     GVKETGSLRQVRIRRSSGTVETVDLYDFLLNGKLDTKSIQVRVGDYVMVPLKAKSVTV
                     RGEVKRAGVYEAIGREGLKDLVRFAGGLGSNALLTRVQVRRYEVNAGERIIDLNLEEI
                     YEGKRADFVLEDGDQVTIFPNVLVRRRLVEILGEGVRRPGIYELTPGMRLDDLVQKAE
                     GLKEGVYLDRVDFIRMNDDLSRTLTQLSFRDLYTEFPPGQYRRSGPDEKNPELRGMDI
                     VQTYSSYEMKGRDKKVYLEGRVKEPGVYVIADNATLYDVLFARGGFQDEEFRRRTYLP
                     LAHIFRKKSGETAEMLIPFSLEKLLEGDARENRTLQSDDRIVIYSFEELASRSTVSIE
                     GLVKKPGSFRFADRMTVQDLILAAGGLTPDAYKVEAVIARSGRGGDAGNGEEAGGEEK
                     LQEKTIVVPIDATVGLQTGEKKTRLEPFDKVTIRNLADWEPLPVVAVTGEALYPGNYT
                     LTSREERLASVVSRAGGFKPDALPEGAVLYRKRNVIDESPAAAAAAAAAPVSINLVEA
                     MRRPGGEHDLILKDGDRLYIPTNPGIVEVRGAVRRPTVLQYQEGAGIRYYVELCGGYL
                     EDADRGRAVVLEPNGTAARVKSVVGFRVSPSVLPGSVIEVPRRPRPEPASEEGSE"
     gene            2214..>2665
                     /locus_tag="P5164_18960"
     CDS             2214..>2665
                     /locus_tag="P5164_18960"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HRY46017.1"
                     /translation="MATPEPGRSEADDYGYVEEPAPIHLIDLLNVVLRRRRLIVVGVF
                     FFVLLAGVVSKRMTPTFTTSAKFLPSKDPDLSSRMTDFVGANDIGSFEKNATSPFYLE
                     LLRSDTFLERIVDRKFRSTRLGGETDLIGFWQVEGASDGERRAKATERL"
BASE COUNT          462 a          816 c         1001 g          386 t
ORIGIN      
        1 aaccagtcct atccgctgac ggtgggggaa gacggcttca tcacgatccc cgacgggggc
       61 gggcgggtct acacgaacgg cgtcagcctg aaggaattcc ggggaatggt cctccggagc
      121 ctctcgcagg cctactcgac gtacatcaac gcccaggatc cggcgcagtc gacggctttc
      181 gtcgacgtga agctcgggaa gcttcggcgg ctcttcgtct tcgtcgtcgg cgaggtgcgc
      241 aagcagggag cgctctcggt cagcccgggc gcggcgacgg tcctcaacct cctgaacaac
      301 gcgggggggg tgaaggagac gggctcgctg cggcaggtgc ggatccgccg gtcgtccggg
      361 acggtcgaga ccgtagacct ctacgacttc ctcctgaacg ggaagctcga cacgaagtcg
      421 atccaggtgc gcgtgggtga ctacgtgatg gtgccgctga aggcgaagtc ggtgacggtg
      481 cgcggcgagg tgaagcgggc aggagtgtac gaggcgatcg ggcgcgaggg gctgaaggac
      541 ctcgtccgct tcgccggagg cctcggctcg aacgcgctcc tcactcgggt ccaggtgcgg
      601 cgctacgagg tgaacgccgg ggagcggatc atcgacctga acctcgagga gatctacgag
      661 gggaagcggg ccgacttcgt tctcgaggac ggagaccagg tgacgatctt cccgaacgtc
      721 ctcgtccgga ggaggctcgt ggagatcctc ggggaaggcg tgcgtcggcc cggcatctac
      781 gagctcacgc cggggatgag gctcgacgac ctcgtccaga aggccgaagg gctgaaggag
      841 ggggtctacc tcgaccgggt cgacttcatc cggatgaacg acgacctctc gcggacgctg
      901 acccagctct cgttccggga cctctacacc gagttcccgc ccgggcagta tcgccgctcg
      961 gggccggacg agaagaaccc cgagctgcgc gggatggaca tcgtgcagac ctactcgtct
     1021 tacgagatga aggggcgcga caagaaggtc tacctggagg gaagggtcaa ggagcccggc
     1081 gtctacgtca tcgccgacaa cgccacgctc tacgacgtcc tcttcgcgcg gggaggtttc
     1141 caggacgagg agttccggcg ccggacgtac ctgccgctcg cgcacatctt ccggaagaag
     1201 agcggggaga cggccgagat gctgatcccc ttcagcctcg agaagctcct cgagggagac
     1261 gcgagggaga accggaccct tcagtccgac gaccggatcg tcatctactc gttcgaggag
     1321 ctggcgtccc gctcgacggt ctcgatcgaa gggctcgtga agaagccggg gagcttccgg
     1381 ttcgcggatc ggatgacggt gcaggacctg atcctcgccg cgggcgggct gacgccggac
     1441 gcctacaagg tggaggcggt catcgcgcgc tcgggccgcg gcggggacgc ggggaacgga
     1501 gaagaggccg gcggcgaaga gaagctccag gagaagacga tcgtcgtccc gatcgacgcg
     1561 acggtgggcc tgcagaccgg agagaagaag acgcggctgg agccgttcga caaggtgacg
     1621 atccggaacc tggccgactg ggagccgctg cccgtggtgg cggtgacggg cgaggcgctc
     1681 taccccggca actacacgct gacctcgaga gaggagcggc tcgcttcggt cgtgtcgcgg
     1741 gcggggggct tcaagccgga cgcgctgccc gagggggcgg tcctttacag gaagcgaaac
     1801 gtcatcgacg aatccccggc ggccgcggcc gcggccgcgg ccgcgcccgt ctctatcaac
     1861 ctggtggagg cgatgcggcg cccgggcggc gagcacgacc tgatcctgaa ggacggcgac
     1921 cggctttaca tcccgacgaa cccggggatc gtcgaggtgc ggggcgcggt gcgccggccg
     1981 accgtgctgc agtaccagga aggcgccgga atccgctact acgtggagct ctgcggcggc
     2041 tacctggagg acgcggaccg ggggcgcgcg gtcgtgctgg agccgaacgg gacggccgca
     2101 cgggtgaaga gcgtcgtcgg cttccgggtg agcccgagcg tcctgccggg cagcgtgatc
     2161 gaggtgccgc gacggccgag gccggagccg gcgagcgagg agggatcgga gtagtggcga
     2221 cgcccgagcc gggccgcagc gaggccgacg actacggcta cgtcgaggag ccggcgccga
     2281 ttcacctcat cgacctgctg aacgtcgtcc ttcggaggcg gcggctcatc gtcgtcgggg
     2341 tcttcttctt cgtcctgctg gccggggtcg tctcgaagcg gatgacgccg acgttcacga
     2401 cgtcggcgaa gttcctcccg tcgaaggacc ccgacctgag ctcgcggatg acggacttcg
     2461 tcggtgcgaa cgacatcggg tcgttcgaga agaacgcgac gtcccccttc tacctcgagc
     2521 tgctccggag cgacacgttc ctcgagcgga tcgtggaccg gaagttccgg agcacccggc
     2581 tcggtgggga gacggatctc atcgggttct ggcaggtcga gggggccagc gacggcgagc
     2641 ggcgggcgaa ggcgacggag aggct
//
LOCUS       DAOTEZ010000532         2663 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_30846_length_2663_cov_4.150690, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000532 DAOTEZ010000000
VERSION     DAOTEZ010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30846_length_2663_cov_4.150690"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..1280)
                     /locus_tag="P5164_18965"
     CDS             complement(36..1280)
                     /locus_tag="P5164_18965"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRY46018.1"
                     /translation="MQTLAALLRERAPGDQLALKDRALDVAAEGVTIADARLPDRPLI
                     YVNEGFERVTGYPAAEALGRNCRFLQGPGTDLVEVERIRAAVAGERECVVEILNYRKD
                     GTTFWNRLSITPVRDDRGEVTHFIGVQSDVTARRLAEEGLRSAKEALEHDLRMAARVQ
                     QSLLPPPEMHLPGLRVARAFHPCTDLGGDAVGVVPLPHDSVGLYLADVSGHGVGAALL
                     SFTLNHLLSPSRDGSVLVEDAGEGPAVVSPARLAGRLNRQFPMGRTRQYFTLVYGAYD
                     GSRGRLRYVVSGHPPPILLPVARPPAPLPGQGLPIGMFEDASWQDETVTLEPGDRIYF
                     YTDGVTEAHDDSEVEFGSARLMAELERVRGLPLREGLDLVADAVREWSGGGPKDDVSL
                     LAIERVNRRTGVEGARAPAGAW"
     gene            complement(1411..2190)
                     /locus_tag="P5164_18970"
     CDS             complement(1411..2190)
                     /locus_tag="P5164_18970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY46019.1"
                     /translation="MDQKKKSVVVAGGTGGIGSEVARGVVREGGNVLLVARDEAKLAE
                     RVAELDALAGRGGAAISFVADAGSQATLNAAAAEAVSRFGRLDGWVHAVGSILLKPLS
                     ATGEEDFARQLDLNAGSAFRALHAALGPMRKQRGGSIVLFGSAAASLGLPNHAAISAA
                     KAAVVGLARSAAMDLARYGIRVNVVSPGLVRTPMSAAITSSEASLKVSQAMHPTGRIS
                     EPADVAAAVLYLLSDGAANVTGAVLPVDGGMAAGRPPALAS"
     gene            complement(2227..>2663)
                     /locus_tag="P5164_18975"
     CDS             complement(2227..>2663)
                     /locus_tag="P5164_18975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46020.1"
                     /translation="RVDGLPPSGIRILRDGGLASRIVENERLGGTSRLSVYGRETFAA
                     ARRDAPDEGVAAELEEAARAALGFPPAAVLERSLKRWRYARAEQPFAGDDPVVAVGGS
                     RLLFAGEAFGDELGVPTAGNTGLERAYLSGLLAAGRVAGFRG"
BASE COUNT          360 a         1036 c          922 g          345 t
ORIGIN      
        1 gctcgacatg gacgtgatct cctgtcggaa aaaggctacc acgcgcccgc gggcgccctc
       61 gcgccctcga cgccggtccg ccggttcacc cgttcgatcg cgaggagcga gacgtcgtcc
      121 ttcgggccgc cgcccgacca ctcccgcacg gcgtccgcga cgaggtcgag cccttccctc
      181 agaggcagcc ccctcacccg ctccagctcg gccatcagcc gtgcggagcc gaactcgacc
      241 tccgaatcgt cgtgcgcctc cgtgacgccg tccgtgtaga agtagatccg gtctccgggc
      301 tcgagcgtca ccgtctcgtc ctgccaggac gcatcctcga acatgccgat cggcagtccc
      361 tggccgggaa gcggcgcggg aggcctggcc acgggcagga ggatgggcgg cgggtgcccc
      421 gagacgacgt accggagccg cccgcgggag ccgtcgtacg ccccgtagac gagcgtgaag
      481 tactgccggg tccggcccat cgggaactgc cggttcaggc gcccggccag gcgcgcgggc
      541 gagacgacgg ccggcccttc gcccgcgtcc tccacgagga ccgacccgtc ccgcgagggc
      601 gagaggaggt ggttcagcgt gaacgagagg agcgcggctc cgacgccgtg cccgctcacg
      661 tccgcgaggt agagccccac cgaatcgtga gggagcggga cgacgccgac ggcgtcgccg
      721 ccgaggtccg tgcaggggtg gaacgcgcgc gcgacccgaa ggcccggaag atgcatctcc
      781 gggggcggga ggagcgactg ctggacgcgg gccgccatcc gcaggtcgtg ttcgagcgct
      841 tccttcgcgc tccggagccc ctcctcggcc agccggcgcg cggtgacgtc cgactgcacc
      901 ccgatgaagt gggtgacctc gccccggtcg tctcgcaccg gcgtgatcga cagccggttc
      961 cagaacgtcg tcccgtcctt ccggtaattc aggatctcga cgacgcactc gcgctccccg
     1021 gcgacggcgg cgcggatcct ctcgacctcg accaggtcgg tgccggggcc ttgcaggaac
     1081 cggcaattcc tccccagcgc ctcggccgcg gggtagccgg tcacgcgctc gaagccctcg
     1141 ttcacgtaga tgaggggccg gtccgggagg cgggcgtcgg cgatcgtgac cccctccgcc
     1201 gcgacgtcga gggcgcggtc cttcagcgcg agctggtccc cgggggctcg ctcccggagg
     1261 agcgccgcga gcgtctgcac tccggcgggt tcgggcggtt cgtgtgagat gcgcgctccc
     1321 tgctctcgat ccgccccgat cataggcggc gcgccgccaa agcggcccgt cgagccgacg
     1381 agctcgttct tcccgcgccg atctctccgt tcaggaggcc agcgccggag gccggcccgc
     1441 cgccatgcct ccgtccaccg gaaggaccgc ccccgtgacg ttcgcggccc cgtccgagag
     1501 gaggtagaga acggccgcgg cgacgtcggc gggctcggag atccgccccg tcgggtgcat
     1561 cgcctgcgac accttcaggc tcgcttcgct cgaggtgatc gccgccgaca tcggcgtccg
     1621 cacgagcccc ggggagacga cgttcacgcg gattccgtag cgggcgaggt ccatcgccgc
     1681 cgagcgggcg aggcccacga ccgccgcctt cgccgccgag atcgcggcgt ggttcggcag
     1741 gccaagggac gccgcggcag acccgaagag gacgatcgac ccgcccctct gcttccgcat
     1801 cgggccgaga gccgcgtgga gcgcgcggaa cgccgagccc gcattgaggt cgagctgccg
     1861 tgcgaagtcc tcctctcccg tcgccgagag cggcttcagc aggatcgagc cgacggcgtg
     1921 gacccagccg tcgagccttc cgaaccggga cacggcctcg gccgcggccg cgttcagggt
     1981 cgcctgcgag cccgcgtccg cgacgaacga gatcgcggcc ccgccgcgtc ccgcgagggc
     2041 gtccagctcc gcgacgcgtt ccgccagctt cgcctcgtcc cgcgccacga ggaggacgtt
     2101 ccctccctcg cgcacgaccc cgcgggccac ctcgctcccg atccctccgg tcccaccggc
     2161 gacgacgacg gacttcttct tctggtccat gtcgtcccct acgccgccgc cactccggcg
     2221 gacgcttcag ccgcggaatc cggcgacccg gccggccgca aggagaccgg acagatacgc
     2281 tcgctcgagt ccggtgttcc ccgccgtcgg caccccgagc tcgtctccga acgcctcgcc
     2341 ggcgaagaga agacgcgagc cgccgaccgc gacgaccggg tcgtcccccg cgaacggctg
     2401 ctccgcccgc gcgtagcgcc agcgcttcag gctccgctcg aggacggcgg ccggcgggaa
     2461 tccgagcgcc gcacgcgcgg cctcctccag ctccgcggcg actccctcgt cgggcgcgtc
     2521 ccggcgggcc gccgcgaacg tttcccgtcc gtagaccgac aggcgcgacg tcccgccgag
     2581 cctctcgttc tcgacgatcc gcgaggccag gcccccgtcg cgcaggatcc ggatgccgga
     2641 tggcggcagg ccgtccacgc gcc
//
LOCUS       DAOTEZ010000533         2657 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_30976_length_2657_cov_5.853958, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000533 DAOTEZ010000000
VERSION     DAOTEZ010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30976_length_2657_cov_5.853958"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="P5164_18980"
     CDS             complement(<1..139)
                     /locus_tag="P5164_18980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019442347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRY46021.1"
                     /translation="MSTKYVFVTGGVVSGLGKGIAGASLAALLEARGFKVTLQKLDPY
                     LN"
     gene            complement(136..906)
                     /gene="kdsB"
                     /locus_tag="P5164_18985"
     CDS             complement(136..906)
                     /gene="kdsB"
                     /locus_tag="P5164_18985"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018869573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HRY46022.1"
                     /translation="MIPSRMGAQRFPGKPLHLIAGVPLVVRVLRRAMESRRADAVLVA
                     TDDERIAAAVREAGGTAVLTPSELPSGTDRVRAAVAGTDAEIVVNVQGDEPLMDPENV
                     DRVIAHLLAHPEVPLATVAKPIDDPAAIADPNVVKVVRGDDGRALYFSRSPIPFRRNA
                     EPGLPTWKHLGLYGYRREALETWTSLPPHPLERAESLEQLRALAAGLVMTVLPAVGDS
                     IGVDTPADAEAVERILLEEAATREAAGARVEREAGGER"
     gene            complement(1206..1640)
                     /locus_tag="P5164_18990"
     CDS             complement(1206..1640)
                     /locus_tag="P5164_18990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46023.1"
                     /translation="MSFQRLLRSLRPAAVRTQAEVTLEDEIALYRRRAEMALREERWN
                     DALVFLAKILRLNPYDVAARMAVAQTYHRGLEEPTKAILTYEKVIAAAGYDESNPYCA
                     SAREGIRELSTSMEEPPFVLNDLVTEEAEQEEIGGLPQTIAL"
     gene            complement(1893..>2657)
                     /locus_tag="P5164_18995"
     CDS             complement(1893..>2657)
                     /locus_tag="P5164_18995"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRY46024.1"
                     /translation="AVLELIHTATLVHDDIIDGADTRRGRVSVNRRWGNELTVLFGDY
                     LYMKGMKVALEEGDVRVLDLLADVTLAMTEGEILGAERRGSADLTIEDYLEVVRRKTA
                     LLFAAACRIPAFLVPGSEKHAEALWGFGLALGTAFQLQDDLLDYTASEEELGKPVLSD
                     LREGKLTLPLILSLADATSEERGAVETVMSERGFVSVPATAILGLVERVGGLEKARGV
                     LEAHAGRARQHALSLPPGDARDALLLAAEYAANRRK"
BASE COUNT          390 a          918 c          946 g          403 t
ORIGIN      
        1 cgttgaggta ggggtcgagc ttctggagag tgaccttgaa gccgcgggcc tcgagcaggg
       61 ccgccaggga agcccccgcg atccccttgc ccaggcccga cacgaccccc ccggtgacga
      121 aaacgtactt cgtgctcatc gctcccctcc cgcctctctc tccacgcgcg cgccggcggc
      181 ctcccgggtc gccgcctctt ccagaaggat ccgctcgacc gcctcggcgt ccgccggcgt
      241 gtcgaccccg atcgagtcgc cgacggcggg gaggaccgtc atcacgagcc ccgcggccag
      301 ggcgcggagc tgctcgaggc tctccgcccg ctcgagcggg tggggcggga ggctcgtcca
      361 ggtctcgagg gcctcgcggc gatagccgta gagcccgagg tgcttccagg tcgggagccc
      421 cggctccgcg ttccgccgga acgggatcgg gctccgcgag aagtagagcg cgcggccgtc
      481 gtcgccgcgg acgaccttga cgacgttcgg gtccgcgatc gcggccgggt cgtcgatcgg
      541 cttcgccacg gtggcgagcg ggacctccgg gtgcgcgagc aggtgcgcga tgacccggtc
      601 gacgttctcg gggtccatga gcggctcgtc gccctgcacg ttcacgacga tctccgcgtc
      661 ggtccccgcc acggcggccc gcacccggtc ggttcccgac gggagctcgg acggcgtcag
      721 caccgcggtc ccgcccgcct cgcggacggc ggcggcgatc cgctcgtcgt ccgtcgcgac
      781 gaggacggcg tcggcccggc gcgattccat cgcgcgccgc aggacgcgca cgacgagcgg
      841 aacgcccgca atgaggtgaa ggggctttcc ggggaagcgc tgggcgccca tccgggacgg
      901 gatcacgacg accgatctac cggggtggcg cacggcgaat ggtaacccat cgattcgcgg
      961 tgccgggtcc cttccccggg tgagccatca cccgaccggg tcgagcggtc ggagcttccc
     1021 cgtccgggcg tcgaggagag cctcgcccct cgtcgccgct tctgcccggc ccccggaatg
     1081 gcccgccacg atgcggatgc gcctcgaagg acccccggaa aagggagcgg cgtcgtcgcc
     1141 gacccgggct ggggggccga cgacgacgcc tcgaggatcc gggaccgggg gcgaaaggga
     1201 gcccgttaca gggcgatggt ctgggggagg ccgccgatct cttcctgctc ggcctcctcg
     1261 gtcacgagat cgttgaggac gaacgggggc tcttccatcg acgtcgagag ctcacggatt
     1321 ccctcacgag cggaggcgca gtaggggttc gactcgtcgt accccgccgc ggcgatcacc
     1381 ttttcgtacg tcaggatggc cttcgtcggc tcctcgaggc cgcggtggta ggtctgggcg
     1441 accgccatcc gcgcggcgac gtcatagggg ttcaaccgga ggatcttcgc caggaagacg
     1501 agggcgtcgt tccagcgctc ctcccggagc gccatctcgg cccgccggcg atagagggcg
     1561 atctcgtcct cgagggtgac ctcggcttgc gtccgcaccg ccgccggccg cagagacctg
     1621 agaagtcgct gaaagctcat cgcttttacc tcgcctggaa tcgcggaccc aaggtaagcc
     1681 agccccggat cgaaatcaag aggatttcca gaatttttta ggctgaaatc gactttcgcc
     1741 ttatttcgat cggagctgta ggcgagcata cggctggtag agcccaggat tcggcgggtg
     1801 ggcgccgatt gccatacgaa attcggagaa gatccggtgg gccgaacaga gatggctccg
     1861 cccaggctgt gccagaagtc cttcgtacgg aactacttgc gtcggttcgc ggcgtactcg
     1921 gccgcgagga ggagggcgtc ccgcgcgtcg ccaggcggca gcgacagggc gtgctgccgc
     1981 gcccggcccg cgtgcgcttc gagaacccca cgagccttct cgaggccacc gactctctcc
     2041 acgaggccga ggatcgcggt ggccgggacg gagacgaatc cccgctcgct catgaccgtc
     2101 tccaccgcgc cccgctcctc ggacgtggcg tccgcgaggc tcaggatcag cggcagcgtc
     2161 agcttccctt cgcgcaggtc ggaaaggacg ggcttcccga gctcctcttc ggacgcggtg
     2221 tagtcgagga ggtcgtcctg gagctggaag gcggtcccga gcgccaggcc gaagccccag
     2281 agagcctcgg cgtgcttctc ggagccgggg acgaggaacg cgggaatccg gcaggccgcg
     2341 gcgaagagga gggcggtctt ccgccgcacg acctcgaggt agtcctcgat cgtcaggtcg
     2401 gccgacccgc gccgctcggc gccgaggatc tctccctcgg tcatggcgag cgtgacgtcg
     2461 gcgaggaggt cgaggacgcg gacgtccccc tcctcgagcg cgaccttcat ccccttcatg
     2521 tagaggtagt cgccgaagag gacggtcagc tcgtttcccc agcggcggtt cacgctgacg
     2581 cggccgcgcc gggtgtcggc gccgtcgatg atgtcgtcgt ggacgagcgt cgccgtgtgg
     2641 atcagctcca ggacggc
//
LOCUS       DAOTEZ010000534         2652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_31070_length_2652_cov_4.943011, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000534 DAOTEZ010000000
VERSION     DAOTEZ010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31070_length_2652_cov_4.943011"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..>2652
                     /locus_tag="P5164_19000"
     CDS             11..>2652
                     /locus_tag="P5164_19000"
                     /inference="COORDINATES: protein motif:HMM:NF040127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HRY46025.1"
                     /translation="MKVFSFEDPCGSTPGTDSSVDDPRLVATYPPAAGSLGNTPISVD
                     PDTGIVYGSSTGSGSSVVHGFDLVPPPLYAVADSDDDGRLERLTEVIPLGVPNPGKRE
                     AVPDPGAPAAGHATYTPESFSVLAFLPGGELETGETIHVDVFATTVTGGRLPESAPGF
                     PKSALRDLVLRRQSDDPSDAAYNRYQSDPIVVVADPRAQYAYARTVAENDPEKPDACR
                     NCVPAADVAAKLRTQPAPDERLEILSGEKIRIRFSESLKASRPYLAEVDLERSALDLT
                     SVRADLTPGLKQAPVLSGNSVLGVATHSREYSASAVDLSLAGRMLDLSIDRTYRSQVL
                     HDGPLGRNQDSALFERLRPLPSGDVEYYDGMARRHTFAYEKDSASYDSPAGIASVLSK
                     SSEGEYVLLHPDRSSTLFDATGRKRAYRDRHATAQSTTDGNRHLFEYDGEGRLASIVD
                     DAGRRTRLVWDEGRLEQVKDFDGRTVDYTLVSARLETVTGPDPQDPSSERPVSRYTWG
                     TASGDLRGRLFSSGRIETVSDGGTNAPATFTVGYAEDGAVSSVTTVDGTTGFTAPSAS
                     TVDVTDPKSATTTFTHQESGQVVKVKDAAGYETEFEYRGSVTKKGAPRADGLPWKTRL
                     PEGQVIENTYGDLGDIDRIRHFNLTSTERRPKPGSSDAPLRTEISYDWRSLPTSVIRR
                     DASTTPRPPLVTTIGRKSNGDVTSISEPGTASVGITTDNFGRPLVVGDSSGRSETRTY
                     DDRPGYTGQLKSTTISGGETAPVSTVYERDARGNVLSTQDGAGRRVTYDVNKLDQTWR
                     ETRGGASPGVTSYRFDATGQVKEQDRVLFTPQGQEPDRSITGYVYDPLGRLKNRSASD
                     SGTTTYGYDGQGNLKTVTTPQGTTT"
BASE COUNT          447 a         1024 c          853 g          328 t
ORIGIN      
        1 gggggaggga atgaaggtct tctccttcga ggacccgtgc ggctcgaccc ccggcacgga
       61 cagctccgtc gacgacccgc gcctcgtggc gacctatcct cccgccgcgg gcagcctggg
      121 gaacacgccg atcagcgtcg accccgacac cggcatcgtc tatggcagct ccaccgggag
      181 cggcagctcg gtcgtccacg gcttcgacct cgttccgccg ccgctctacg cggtggccga
      241 ctcggacgac gacggccggc tcgagcgcct gaccgaggtc atcccgctcg gcgtcccgaa
      301 ccccgggaag agggaagccg ttccggatcc gggtgcgccg gcggccgggc acgcgacgta
      361 cacccccgag tcgttcagcg ttctcgcctt cctcccgggc ggcgagctcg agacgggaga
      421 gaccatccac gtcgacgtct tcgcgacgac cgtcacgggc gggcgcctgc ccgagagcgc
      481 tcccggcttc ccgaagagcg cgcttcgcga cctcgtcctc cggcggcaga gcgacgatcc
      541 ctccgacgcg gcctacaacc gctaccagtc ggacccgatc gtcgtcgtcg ccgacccgcg
      601 ggcgcagtac gcctacgccc gcaccgtcgc cgagaatgac cccgagaagc ccgacgcctg
      661 ccggaactgc gtccccgcgg cggacgtcgc cgcgaagctc cggacgcagc cggctccgga
      721 cgagcgcctc gagatcctct ccggcgagaa gatccggatc cggttctcag agtcgctcaa
      781 ggcctcccgg ccgtacctcg cggaggtcga cctcgagagg agcgctctcg atctgacctc
      841 cgtccgggcc gacctcaccc ccggcctgaa gcaggccccg gtcctctcgg ggaacagcgt
      901 cctcggcgtc gcgacccact cccgcgagta ctcggcctcg gccgtggacc tgagcctcgc
      961 cggccggatg ctcgacctct cgatcgaccg cacctaccgc agccaggtcc tccacgacgg
     1021 ccctctcggc cgcaaccagg acagcgccct cttcgagcgc cttcggccgc tcccctcggg
     1081 cgacgtcgag tactacgacg gcatggcgcg gagacacacc ttcgcctacg agaaggactc
     1141 ggcgtcgtac gactcgccgg cgggaatcgc gtcggtcctc tcgaagagct cggagggcga
     1201 gtacgtcctc ctccaccccg acaggtcgag caccctcttc gacgccacgg gccgcaagcg
     1261 ggcctaccgc gaccggcacg ccacggcgca gagcacgacc gacggcaacc gccacctctt
     1321 cgagtacgac ggcgaagggc gcctcgcctc gatcgtcgac gacgccgggc ggcggacgcg
     1381 gctcgtctgg gacgaggggc gactggagca ggtgaaggac ttcgacggcc ggaccgtcga
     1441 ctacaccctc gtttccgcgc gcctcgagac cgtgaccggc cccgacccgc aggatccctc
     1501 gagcgagcgg ccggtgagcc ggtatacgtg gggcacggcg tcgggagacc tgcgcgggcg
     1561 gctcttcagc tcgggccgca tcgaaaccgt ctccgacggt gggacaaacg ctccggcaac
     1621 gttcacggtc ggctatgccg aggacggggc cgtctcgagc gtcacgaccg tcgacggcac
     1681 gacgggcttc accgcacctt ccgccagcac cgtcgacgtc accgacccga agagcgcgac
     1741 gaccaccttc acccatcagg agtctggaca ggtcgtgaag gtcaaggacg cggccggcta
     1801 cgagaccgag ttcgagtaca ggggcagcgt cacgaagaag ggcgcgccga gagcggacgg
     1861 cctcccgtgg aagacgaggc tccccgaggg ccaggtcatc gagaacacat acggcgacct
     1921 cggcgacatc gaccggatcc gccacttcaa cctgacgtcg accgagcgcc ggccgaagcc
     1981 gggctcgtcc gacgcccctc ttcggaccga gatctcctac gactggagga gcctcccgac
     2041 ctccgtcatt cggcgcgacg cctcgacgac gccccgcccg ccgctcgtca cgacgatcgg
     2101 ccgcaagtcg aacggcgacg tcacctcgat cagcgagccg ggcacggcga gcgtcggcat
     2161 cacgaccgac aacttcggcc ggcctctcgt cgtcggcgac tcctccggcc ggagcgagac
     2221 ccgcacctac gacgaccgcc ccggctacac cggccagctg aagtcgacga ccatctccgg
     2281 gggagagacg gcgcccgtct cgaccgtcta cgagcgcgac gcccgcggca acgtcctctc
     2341 cacgcaggac ggagccggcc gacgcgtcac ctacgacgtc aacaagctcg accagacctg
     2401 gcgggagacc cgcggaggcg cctcgccggg ggtcacgagc taccgcttcg acgccacggg
     2461 ccaggtgaag gagcaggacc gggtcctctt cactccccag ggccaggagc cggaccgctc
     2521 catcacgggc tatgtatacg accccctcgg ccggctgaag aaccgctcgg cctccgactc
     2581 cggcaccacc acctacggct acgacggcca gggcaacctg aagaccgtga cgaccccgca
     2641 ggggacgacg ac
//
LOCUS       DAOTEZ010000535         2650 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_31110_length_2650_cov_7.314836, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000535 DAOTEZ010000000
VERSION     DAOTEZ010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31110_length_2650_cov_7.314836"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..942
                     /gene="lepB"
                     /locus_tag="P5164_19005"
     CDS             214..942
                     /gene="lepB"
                     /locus_tag="P5164_19005"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRY46026.1"
                     /translation="MAISPEKRKQILKDVRQLVLMAAIILTGRSVLADWYVVPTGSMK
                     PTILEGDRVFVWKSAYQIRVPFSKIRLMTTGEPRRGDVVVVRNPDGGSVPFVKRLIGL
                     PGDVVELRNEALLVNGKPQKVEYLEPLTLPDGDIEILGTERIDGRTHPIRILPERPAL
                     RNFGPITVPEGEVFLMGDNRDESRDARFFGTRPVVDLLGRAVGVMWSWSPARTDGSAL
                     SRLGRPRFSRFGRAFITSAPAARE"
     gene            1015..1509
                     /locus_tag="P5164_19010"
     CDS             1015..1509
                     /locus_tag="P5164_19010"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRY46027.1"
                     /translation="MAPPALECLLRARGLRVTEPRLAVLAYLAGTDRHPTAEEVESAV
                     NAAGPVLSRASVYNVLHALVDVGLVTGLSVKDGPVRYDAHLGPHHHLVCRGCGRVEDV
                     EWKDFAVAERGVLPDGRAVRTLFLTLDGLCTGCGEKEEGRGEAASPLPSRRPGDAQRF
                     STGT"
     gene            complement(1485..>2650)
                     /locus_tag="P5164_19015"
     CDS             complement(1485..>2650)
                     /locus_tag="P5164_19015"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HRY46028.1"
                     /translation="MTYDPAFLNTASCKSKITFIDGDKGILEYRGYPIEQLAEKSSFL
                     EVAWLLWNGELPTKGELDEFTRHVTHHTYLHENIKKLMEGFRHDAHPMGMLQSTVGAL
                     STFYPSAKQVNDPRQRAHHTIRLIAKMPTIAAFAYRHSIGFPYVYPDNELSYVGNFLS
                     MLRKMSEPKYKVNPVLERALDVLFILHADHEQNCSTNAMRAISSAQTDPYSSLSGAIS
                     ALYGPLHGGANEAVLTMLREIGTINNVPAFVKEVKEGQGEKKLMGFGHRVYKNYDPRA
                     KVIKGLADQVFEVTGKNPLLTIAVELERIALQDDYFVKRKLYPNVDFYSGLIYEAMGF
                     PPDYFTVLFAIGRTAGWLAQWHEMVNDPDQKIARPRQVFLGPARRDYVPVEKR"
BASE COUNT          397 a          854 c          941 g          458 t
ORIGIN      
        1 agtcggggag tccggtcttc gggcagatcg aggtgaactc cgggatcgtg atcgtgacct
       61 cgtagtcgtc ctcgtactgc gagggccacg tctcgaggga cgggagctcc gccttcgtcc
      121 ccgaggcggc gtggtcgtcc gtgtagccgg tcgtcatggc ggaaggatag cccggccgtc
      181 ccgcggcacg gaatgtgcga cactccgcgg ccgatggcca tttcgcccga gaaacgcaag
      241 cagatcctga aggacgtgag gcagctcgtc ctcatggcgg cgatcatcct gacggggcgt
      301 tcggtcctcg cggactggta cgtcgtcccg accgggtcga tgaagccgac gattctggag
      361 ggggaccgcg tcttcgtctg gaagtccgcc taccagatcc gcgttccgtt ctcgaagatc
      421 cggctcatga cgaccggcga gccgcgacgg ggagacgtcg tcgtcgtccg caacccggac
      481 ggcgggagcg ttcccttcgt caagaggctg atcgggctcc cgggcgacgt cgtcgagctg
      541 cggaacgagg cgctcctcgt caacggaaag ccccagaagg tggaatacct cgagccgctc
      601 acgctgccgg acggcgacat cgagatcctc ggcaccgagc ggatcgacgg acgcacgcac
      661 ccgatccgga tcctgccgga acgccctgcc ctccggaact tcggcccgat cacggtgccg
      721 gaaggggagg tcttcctgat gggagacaac cgcgacgaga gccgcgacgc ccgcttcttc
      781 gggacgcggc cggtcgtcga cctcctcggg cgggccgtcg gcgtcatgtg gagctggagc
      841 cccgcccgga ccgacggatc agcgctctcc cgactcggcc ggccccggtt ctcccgcttc
      901 ggccgggcct tcatcacgtc cgctcccgcc gcccgggagt aggcccggcg tccggctccg
      961 gggcggagcc gcttgtcatc gccgtcgttt ggatttattc taaatagcgt gaagatggcg
     1021 ccgcccgccc tggaatgcct gctccgcgcc cgggggcttc gggtgaccga gcctcggctg
     1081 gcggtgctcg cctacctcgc cgggacggac cgtcacccga ccgctgaaga ggtggagagc
     1141 gccgtgaacg cggcggggcc ggtcctctcg cgcgcctcgg tctacaacgt cctccatgcg
     1201 ctcgtggacg tcgggctcgt gacgggcctc agcgtgaagg acgggccggt ccgctacgac
     1261 gcccacctcg ggccgcacca ccacctcgtc tgccggggct gcggccgggt cgaggacgtc
     1321 gagtggaagg acttcgcggt ggccgagcgc ggcgtcctgc ccgacggccg cgcggtccgc
     1381 accctcttcc tgaccctcga cgggctctgc acgggctgcg gcgaaaagga agaggggaga
     1441 ggcgaagccg cctcccccct cccttcccgg cgacccgggg acgctcagcg cttctcgacg
     1501 ggaacgtagt cgcgccgggc cggtccgagg aagacctggc gcggccgggc gatcttctgg
     1561 tcggggtcgt tgaccatctc gtgccactgg gcgagccagc cggcggtccg tccgatcgcg
     1621 aagaggacgg tgaagtagtc gggggggaag cccatcgcct cgtagatgag gcccgagtag
     1681 aagtcgacgt tcggatagag cttcctcttg acgaagtagt cgtcctggag cgcgatccgc
     1741 tcgagctcga cggcgatcgt caggagaggg ttcttcccgg tgacttcgaa gacctggtcg
     1801 gcgagcccct tgatgacctt cgcgcgcggg tcgtagttct tgtagacccg gtgcccgaag
     1861 cccatcagct tcttctcgcc ctgcccctcc ttgacctcct tgacgaaggc cgggacgttg
     1921 ttgatggtcc cgatctcgcg aagcatcgtg aggacggcct cgttcgcgcc gccgtggagc
     1981 gggccgtaga gcgcggaaat ggcgccggag aggctggagt acgggtcggt ctgcgcggag
     2041 ctgatcgccc gcatcgcgtt cgtcgagcag ttctgctcat ggtcggcgtg gaggatgaag
     2101 aggacgtcga gcgcccgctc gaggaccggg ttcaccttgt acttcggctc cgacatcttc
     2161 cggagcatcg agaggaagtt gccgacgtag gagagctcgt tgtcggggta gacgtagggg
     2221 aagccgatgg agtgccggta ggcgaacgcg gcgatcgtcg gcatcttcgc gatgagccgg
     2281 atcgtgtggt gggcgcgctg gcgcgggtcg ttcacctgct tggcagaggg gtagaacgtc
     2341 gagagcgcgc cgaccgtcga ctggagcatc cccatcgggt gcgcgtcgtg acggaaaccc
     2401 tccatgagct tcttgatgtt ctcgtggagg taggtgtggt gggtgacgtg gcgggtgaac
     2461 tcgtcgagct cgcccttcgt cggcagctcg ccgttccaga ggagccaggc gacctccagg
     2521 aagctcgact tctcggcgag ctgctcgatc gggtagccgc ggtactcgag gatccccttg
     2581 tcgccgtcga tgaacgtgat cttcgacttg cacgacgccg tgttcaggaa cgcggggtcg
     2641 tacgtcatca
//
LOCUS       DAOTEZ010000536         2617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_31768_length_2617_cov_4.752927, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000536 DAOTEZ010000000
VERSION     DAOTEZ010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31768_length_2617_cov_4.752927"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="P5164_19020"
     CDS             complement(<1..325)
                     /locus_tag="P5164_19020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY46029.1"
                     /translation="MASERVRGAGAVPADRGAALLVVDDNEVNRDLLSRRLVAAGFAV
                     AGAASGPEALTALDEGRFDLVLLDVMMPEMDGLEVLRRIRARFGVGELPVVMASALGD
                     TRNLVD"
     gene            complement(376..1527)
                     /locus_tag="P5164_19025"
     CDS             complement(376..1527)
                     /locus_tag="P5164_19025"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRY46030.1"
                     /translation="MPRPPRLAPIPLSMKGSVYSTVLDRLAACPGETYPLHVGDTYLE
                     PASLCRMEDLAVADHPGMHRYAPPQGLPALVDAVVERLRARTGVPLERANVFVAGGAT
                     SGLACVAGAIVAPGDEVAILAPHWPLIEGHVRMAGGVPVDVPFFGAVDSRESAVAAVE
                     AALTPRTVALYVNTPSNPTGHVVPRAWLEGLVDVARRHDLWLLFDEVYEDYVYEGEHV
                     PGLSLAPERSFSVHSCSKAFGMAGNRVGWIAGPAAPIVEALKLSTHTAYAAPTAAQLA
                     ALRVLRGPGDAWVERTRAAYASAGAAAAERLGVPRPQGSTFLFLDVAPRLDARGLTGF
                     LEDAADRGLAVAPGPSFGPCPTHVRVCYTSAPPDVVRRGIEVLAALVGR"
     gene            1600..>2617
                     /locus_tag="P5164_19030"
     CDS             1600..>2617
                     /locus_tag="P5164_19030"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRY46031.1"
                     /translation="MTHEEPLPPFRPAPALGQRHVQTFWGRLTRPRLLVPLRRELLAT
                     PDGDELVLDRLDGPPGAPLVVLLHGLEGSAHSVYVQGLLALLARRSLRAVALNFRSCA
                     RDLPSLRRNLPNRGRRLYHSGETADLDHLLRTLAAAEPGVALGAIGVSLGGNVLLKWL
                     GEHPEERLVAAASTISVPFDLEAGARELEGGLGPVYTATFLRGLRTKARQLLRRHPDL
                     ASRIDLGRLRRARTFFEFDDAVTGPLHGFRGAKHYYDESSAIGWVGRVTTPTLCLSAE
                     DDPFLPAEALDRVAARASGAVRLVRTRQGGHTGFIAGASPLRVRYWAEERAAEFVAAR
                     LASPL"
BASE COUNT          325 a         1012 c          971 g          309 t
ORIGIN      
        1 cgtcgacgag gttccgggtg tccccgaggg cgctcgccat gacgacgggg agctctccga
       61 cgccgaagcg cgcgcggatg cggcgcagga cctcgaggcc gtccatctcc ggcatcatca
      121 cgtccaggag gacgaggtcg aagcgcccct cgtcgagcgc cgtcagggcc tcgggccccg
      181 aggccgcgcc cgcgacggcg aaaccggcgg cgacgaggcg gcgcgacagc aggtcccggt
      241 tcacctcgtt gtcgtcgacg acgaggaggg ccgccccccg gtccgccggg acggctccgg
      301 ccccgcggac cctttcggac gccatcgcgc gcgctccttc cgttcggagc gggaacgata
      361 gcaccggaga gcccgtcagc ggccgacgag cgcggcgagg acctcgatgc cgcgccggac
      421 gacgtcgggc ggcgcggacg tgtagcagac cctcacgtgc gtcgggcagg gcccgaagct
      481 gggtccgggg gcgacggcga ggccgcggtc ggccgcgtcc tccaggaacc ccgtcagccc
      541 ccgcgcgtcg aggcgcgggg cgacgtcgag gaagaggaac gtcgagccct gcggccgcgg
      601 gacgccgagc cgctccgcgg ccgcggcgcc ggccgaggcg tacgccgcgc gggtcctctc
      661 gacccaggcg tcgcccgggc cccggaggac tcggagggcc gcgagctgcg ctgcggtcgg
      721 cgcggcgtag gccgtgtgcg tcgagagctt cagcgcctcg acgatgggcg cggccgggcc
      781 ggcgatccag ccgacccggt tccccgccat gccgaacgcc ttcgagcagg agtggacgga
      841 gaaggaacgc tccggcgcga gcgagaggcc cggcacgtgc tctccctcgt agacgtagtc
      901 ctcgtagacc tcgtcgaaga ggagccagag gtcgtgccgg cgggcgacgt cgacgagccc
      961 ctcgagccac gcgcgcggga cgacgtgccc ggtcgggttg ctcggcgtgt tcacgtagag
     1021 cgccacggtc cgcggcgtga gcgccgcctc gacggcggcg accgccgact cccgcgaatc
     1081 gacggccccg aagaaaggga cgtcgacggg gaccccgccc gccatccgga cgtgcccctc
     1141 gatcagcggc cagtgcggcg cgaggatcgc cacctcgtcc cccggcgcga cgatcgcccc
     1201 ggcgacgcag gcgaggcccg acgtggcgcc gccggcgacg aagacgttcg cccgctcgag
     1261 cgggacgccc gtccgcgcgc ggagccgctc gaccaccgcg tcgacgaggg ccgggagccc
     1321 ctgcggcggg gcgtaccggt gcatccccgg gtggtccgcg acggcgaggt cctccatccg
     1381 gcagagggag gccggctcga ggtacgtgtc gccgacgtgc agcgggtagg tctccccggg
     1441 gcaggccgcg agccgatcga ggacggtgga gtagacggag cccttcatcg agagcgggat
     1501 cggggcgagg cggggcgggc gcggcatgga ggcgggatgt tagccgggag ccgcccgcac
     1561 gtccccggcc taaactccgc gacgccgcct cgcccgccga tgacgcacga agagccgctc
     1621 cccccgttcc gccctgcccc cgcgctcggg cagaggcacg tccagacctt ctggggccgg
     1681 ctgacgcgcc cgcgcctcct cgtgcccctc cggcgcgagc tgctcgcgac gcccgacggc
     1741 gacgagctgg tcctcgaccg cctcgacggg ccgcccggcg cgccgctcgt cgtcctcctc
     1801 cacggcctcg aggggagcgc ccactccgtc tacgtgcagg ggctcctcgc cctcctcgct
     1861 cgtcgctcgc tccgggcggt tgcgctgaac ttccgctcct gcgcgcgcga cctcccctcg
     1921 ctccgccgga acctcccgaa ccgcgggagg cgcctctacc actcggggga gacggcggac
     1981 ctcgaccacc tcctccgtac gctcgccgcc gccgagccgg gcgtcgccct gggggcgatc
     2041 ggcgtctcgc tcggcgggaa cgtcctcctc aagtggctcg gcgagcatcc ggaggagcgg
     2101 ctcgtcgctg ccgcgtcgac gatctcggtc ccgttcgacc tcgaggcggg cgcgcgggag
     2161 ctggaggggg ggctcggccc cgtctacacc gcgacgttcc tccgcggcct ccggacgaag
     2221 gcgcggcagc tcctccgccg tcatcccgac ctcgcctccc ggatcgacct cggccggctc
     2281 cggagggccc gcacgttctt cgagttcgac gacgccgtca ccggccccct ccacggcttc
     2341 cgcggagcga agcactatta cgacgagtcg agcgcgatcg gctgggtggg ccgggtcacg
     2401 acgccgacac tctgcctctc ggccgaggac gacccgtttc tccccgccga ggcgctcgac
     2461 cgcgtggccg cgcgcgcctc gggcgcggtc cgcctcgtcc ggacgcgcca gggagggcac
     2521 accggcttca tcgcgggggc gagcccgctc cgggtccgtt actgggccga ggagcgcgcg
     2581 gcggagttcg tcgccgcgcg cctcgcgtct cccctca
//
LOCUS       DAOTEZ010000537         2616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_31788_length_2616_cov_5.392034, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000537 DAOTEZ010000000
VERSION     DAOTEZ010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31788_length_2616_cov_5.392034"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="P5164_19035"
     CDS             complement(<1..461)
                     /locus_tag="P5164_19035"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HRY46032.1"
                     /translation="MFEKYNEKARRALFFARYEASKLGSKVIESEHVLLGILREGEDV
                     TREIFARFNVKPDDVRKEIEGDRVFVERVSSSQELPLSEESKKILAYASHEAESMMHP
                     YVGTEHLLIGILRVDPCVASRLLTGHGFNLYGVREEAIALIKEREASKQKK"
     gene            complement(548..1231)
                     /locus_tag="P5164_19040"
     CDS             complement(548..1231)
                     /locus_tag="P5164_19040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY46033.1"
                     /translation="MPAEPLLSAESLAKGFGEGEARVELFSGLSLELGAGEAAAIVGP
                     SGCGKSTLLHLLAGLDSPDAGRVLVDGRDWTTLPPEPRAKWRGEAVGFVFQLHHLLPE
                     LTAEENVALPLLLAGSPEGPARSAARELLSRVGLSRQAGAFPRVLSGGERQRVAIARA
                     VVRSPRLLLCDEPTGSLDAAHAADVLDLLLGAARERAGALLLVTHDPGVASRCGSVRT
                     LTPAGLSAG"
     gene            complement(1221..2312)
                     /locus_tag="P5164_19045"
     CDS             complement(1221..2312)
                     /locus_tag="P5164_19045"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY46034.1"
                     /translation="MGVVALAAFVGLVLGVAALVGALALLSGFQSHVLSRLAEETPHV
                     LVTPAGRPDFAGEEEAGQALAALPGVEGVVPVVRGRGWITARGQAVPAVLAGREGADG
                     ITLDPSQARQLSALPGEEVVLVSSRSRLSPIGPVPITASLPVTDVAVVSTGRHQPEAV
                     LPADTARRLFGLPAAGATGYELRLADPDAAGRVAARAREALGAAGTATTTWQEANRPL
                     LLALKLERLAIFATVFLVVVVAGLNLAATSAVLAATRRSDAAVLTVLGATPALLARVF
                     LLAGLLLGAAGTLCGLAVGALLAIVLDATKALPLPAQLFSLAHVPFRVTPRDLLAVGT
                     FSLIWSFVASLVPARMAARVDVAEALRAG"
     gene            complement(2366..>2616)
                     /locus_tag="P5164_19050"
     CDS             complement(2366..>2616)
                     /locus_tag="P5164_19050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014993876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRY46035.1"
                     /translation="FSELNDPDEQAEKFRAQVAERTKGDDEAMLFDEDYVEALSYGMP
                     PTAGEGVGIDRLAMLFTNSRSIRDVILFPLMRPKAAGR"
BASE COUNT          393 a          936 c          987 g          300 t
ORIGIN      
        1 tccttcttct gcttggaggc ctcgcgctcc ttgatgaggg cgatcgcctc ctccctcact
       61 ccgtagaggt tgaagccgtg cccggtcagg aggcgggagg cgacgcacgg gtcgacccgg
      121 aggatcccga tgaggaggtg ctccgtcccg acgtaggggt gcatcatcga ctccgcctcg
      181 tgggaggcgt aggcgaggat cttcttcgac tcctcggaga gcgggagctc ctggctcgag
      241 gagacgcgct cgacgaagac ccggtctccc tcgatctcct tccggacgtc gtcgggcttc
      301 acgttgaagc gggcgaagat ctcgcgggtg acgtcctccc cctcccgcag gatgccgagg
      361 aggacgtgct ccgactcgat caccttcgag ccgagcttcg aggcctcgta tcgggcgaag
      421 aagagggcgc ggcgcgcttt ctcgttgtac ttctcgaaca tggctcgacc tcgacgaggc
      481 cgctcggaaa gcgggcttcg accgtctccc gatcctagca cagggggtgc catcgaccgg
      541 gcgccgctca gccggcggag aggcccgccg gggtcagcgt ccgcaccgat ccgcaccgcg
      601 acgccactcc ggggtcgtgc gtgacgagga ggagcgcacc cgcccgctcg cgcgccgcgc
      661 cgaggaggag atcgaggacg tcggcggcat gcgccgcgtc gaggctcccg gtcggctcgt
      721 cgcagaggag gagccgcggg ctcctcacga cggcccgggc gatcgcgacg cgctggcgct
      781 cgccgcccga gaggacgcgc gggaaggcgc ccgcctgacg ggagagcccg acccgggaga
      841 ggagctcgcg tgccgcggag cgggccgggc cctccggcga gccggcgagg aggagcggga
      901 gggcgacgtt ctcctcggcg gtgagctcgg gaaggaggtg gtggagctgg aagacgaaac
      961 cgacggcttc gccgcgccac ttcgccctcg gctccggggg cagggtggtc cagtcccgcc
     1021 cgtcgacgag gacgcggccg gcgtccgggc tgtcgaggcc cgcgaggagg tggaggagcg
     1081 tcgacttgcc gcagcccgac ggtccgacga tcgccgccgc ctcgcccgcg ccgagctcca
     1141 gcgagaggcc ggagaagagc tcgacgcggg cctcgccctc gccgaagccc ttcgcgagcg
     1201 actcggccga gagcagcggc tcagccggca cgaagagcct ccgcgacgtc gacgcgcgcc
     1261 gccatccgcg ccgggacgag cgaagcgacg aacgaccaga tcagcgagaa cgtcccgacc
     1321 gcgaggaggt ccctcggggt cacgcggaac ggcacgtgcg cgagagagaa gagctgcgcg
     1381 gggagcggga gcgccttcgt cgcgtcgagg acgatcgcga ggagcgcgcc cacggcgagg
     1441 ccgcagagcg tcccggccgc gccgaggagg aggccggcga ggaggaagac gcgcgcgagg
     1501 agcgcgggcg tcgcgccgag caccgtgagg acggcggcgt cgctccggcg ggtcgccgcg
     1561 aggacggccg acgtggccgc caggttcagg ccggcgacga cgacgacgag gaagaccgtc
     1621 gcgaagatcg ccagcctctc gagcttcagc gcgagcagga gcggccggtt cgcctcctgc
     1681 cacgtcgtcg tcgccgtgcc cgccgcgccg agcgcctccc gtgcgcgagc cgcgacccgg
     1741 cccgccgcgt cggggtcggc gagccggagc tcgtaccccg tggcgccggc cgccgggaga
     1801 ccgaagagac ggcgtgccgt gtcggcagga aggaccgctt ccggctggtg ccgccccgtc
     1861 gacacgaccg ccacgtccgt gaccggaaga gaggccgtga tcgggaccgg gccgatcgga
     1921 gagagccggg aacgcgagga gacgaggacg acctcctcgc ccgggagagc cgaaagctgc
     1981 cgggcctggg acgggtcgag cgtgatcccg tccgccccct cccgccccgc gaggacggcg
     2041 ggaaccgcct gtccgcgggc cgtgatccag ccgcgccccc tcacgaccgg cacgacgccc
     2101 tcgacgcccg gcagcgcggc gagcgcctgc cccgcctctt cctcccccgc gaaatcggga
     2161 cgccccgccg gcgtgacgag gacgtgcggc gtctcctcgg cgagcctcga gaggacgtgc
     2221 gactggaagc ccgagaggag cgcgagcgcg ccgacgaggg ccgcaacgcc caggacgagc
     2281 ccgacgaacg cggccagcgc gacgacgccc acctgcgcat cccgcctcga ggcgaggagg
     2341 tagcggcggg cgagcagggc ttcggtcagc gccccgccgc cttcggccgc atgagcggga
     2401 agaggatcac gtcccggatc gagcgcgagt tcgtgaagag catcgcgagg cggtcgatcc
     2461 cgaccccttc ccccgccgtc gggggcatgc cgtacgagag cgcctcgacg tagtcctcgt
     2521 cgaagagcat tgcctcgtcg tcccccttcg tccgctcggc gacctgcgcg cggaacttct
     2581 ccgcctgctc gtcggggtcg ttcagctccg agaacc
//
LOCUS       DAOTEZ010000538         2603 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_32050_length_2603_cov_5.725275, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000538 DAOTEZ010000000
VERSION     DAOTEZ010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32050_length_2603_cov_5.725275"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="P5164_19055"
     CDS             complement(<1..473)
                     /locus_tag="P5164_19055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HRY46036.1"
                     /translation="MAKGAGSPGKAIRDAWERCRRIPAGERLFSWALGRMVPYSGSIG
                     AVVRDLSPGRARVELADRRRVRNHLDSIHAVALMNLAELSTGLALNTALPDDARAILV
                     RLTIEYVKKARGTLTSECFLSPVTTNERREVEVAAEIRDAAGEVVARAAALWLVGP"
     gene            541..1500
                     /locus_tag="P5164_19060"
     CDS             541..1500
                     /locus_tag="P5164_19060"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRY46037.1"
                     /translation="MLLPPTPAAIRRAAAALRRGEPVAFPTETVYGLGANALDPVAVR
                     RVFAAKGRPDDNPLIVHVTGLPMLRGLVRSLPPAARALAERFWPGPLTLVVPKTRAVP
                     DAVTAGLPTVAVRVPDHPVALALIEAAGVPVAAPSANRSGRPSPTRAAHVAADFPGLL
                     VLDGGAARHGLESTVVSPGDPPRVLRLGAIPVEELRAVLPGLVVATRTRARAESPGMR
                     HRHYAPARPLVLFRRSRAAALREALAGDPKALLLCADADAPLFPGRRPVRLGGTPAQI
                     AHGLFDALRSERRGSVLFAYALPRRGLLRAVMDRLERAASRVV"
     gene            complement(1516..>2603)
                     /locus_tag="P5164_19065"
     CDS             complement(1516..>2603)
                     /locus_tag="P5164_19065"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HRY46038.1"
                     /translation="EKLDSRDVADLYTVEWADAREVLLSEHRDAIERERPGFRRGLLK
                     VSALLHGLVRRLSPVRRLVVLFVLLGVLLSTIKLVGRGWPADAKGTVLSLLLCLFLLL
                     VLLGLELVDKLRFRDELVLARQLQAELVPVSPPVVPGWELGAFNRVANTVGGDIYEFQ
                     PLSDGRLAVLFGDASGHGMAAGLVMAVAHTAWRLQVERDASPAAVLATLNRILCRAGS
                     CRVAGPRQFFAGVAILLGPDGSFEVAVAGHPPMLRVDARGAVVEKFGRGAYPVGIKEA
                     QAWAVETGAVAPGESLVLHSDGLPEARNVLGQEFGDERILAVLARTPGRGAAEIVAAL
                     SGEVLSFLGRTAIGDDVSIAVLKRRSS"
BASE COUNT          330 a         1022 c          942 g          309 t
ORIGIN      
        1 ggcccgacga gccagagggc cgccgctctc gccacgacct cccccgcggc gtcgcggatc
       61 tccgccgcga cctccacctc gcgccgctcg ttcgtcgtaa caggcgaaag gaagcactcg
      121 gacgtgagcg tgccgcgcgc cttcttcacg tactcgatcg tcagcctcac gaggatcgcg
      181 cgggcgtcgt cggggagggc ggtgttcagc gccaggccgg tcgagagctc cgcgaggttc
      241 atcagcgcga ccgcgtggat cgagtcgaga tggttcctca cgcggcggcg gtcggcgagc
      301 tccacgcggg cgcgtcccgg ggagaggtcg cggacgaccg cgccgatcga gccggagtag
      361 gggaccatcc ggccgagcgc ccagctgaag agcctctctc ccgccgggat cctccggcag
      421 cgttcccacg cgtcccggat cgccttcccc ggcgagcccg cccctttcgc catcccgcct
      481 cctccgcgcg ccggcgcgcg ggcccattct gcccgaaccc gcgtagactc cccgcccgcc
      541 gtgctcctcc cgcccacgcc cgccgcgatc cgccgcgccg ccgccgcgct ccgaaggggc
      601 gagccggtcg cgttcccgac ggagacggtc tatggcctcg gcgcgaacgc tctcgacccg
      661 gtcgcggtcc ggcgggtctt cgcggcgaag gggcgcccgg acgacaaccc gctcatcgtc
      721 cacgtcacgg gcctcccgat gctccgcggc ctcgtccgct ccctcccgcc cgcggcccgc
      781 gcgctggcgg agaggttctg gcccggcccg ctcacgctcg tcgtcccgaa gacgcgcgcg
      841 gtccccgacg cggtcacggc cgggctcccg acggttgcgg tccgggtccc cgaccaccct
      901 gtggcgctcg cgctcatcga ggccgcgggg gtccccgtcg cagcgccgag cgcgaaccgc
      961 tccgggcgcc cgagcccgac ccgcgccgcg cacgtcgcag ccgacttccc cggccttctc
     1021 gtcctggacg gcggggcggc gcgccacggc ctcgagtcga ccgtcgtctc ccccggcgat
     1081 cctccccgtg tccttcggct cggggcgatc ccggtcgagg agctccgggc cgtcctcccc
     1141 ggcctcgtcg tggccacgag gacgcgcgca cgcgccgaga gcccggggat gcggcaccgg
     1201 cactacgcgc cggcccggcc gctcgtcctc tttcggcgct cgcgcgccgc cgcgctccgc
     1261 gaggcgctcg ccggcgaccc gaaggcgctc ctcctgtgcg ccgatgccga cgcgccgctc
     1321 tttcccggcc ggcggcccgt ccgcctcggc gggacgcccg cccagatcgc gcacggcctc
     1381 ttcgacgcgc tccggtcgga gcggcgcggg agcgtcctct tcgcctacgc cctcccgcgg
     1441 cggggcctgc tgcgggcggt gatggaccgg ctggagcgcg cggcgtcccg cgtcgtctga
     1501 cgcggtcacc ctccgtcagg aggaccggcg cttcaggacg gcgatcgaga cgtcgtcccc
     1561 gatcgccgtc cgccccagga acgagaggac ctcgcccgag agcgctgcga cgatctcggc
     1621 cgctccacgc ccgggcgtcc gcgcgagcac cgcgaggatt cgctcgtccc cgaactcctg
     1681 gccgagcacg ttccgggcct cgggaaggcc gtcggagtgg aggacgagcg actcgcccgg
     1741 cgcgacggct cccgtctcga cggcccaggc ctgcgcctcc ttgatgccga ccgggtaggc
     1801 gccccggccg aacttctcga cgacggcccc gcgcgcgtcg acccgcagca tcggaggatg
     1861 cccggccacg gcgacctcga acgacccgtc agggccgagg aggatcgcga ccccggcgaa
     1921 gaactggcgg ggcccggcga cacggcagct cccggcccgg cagaggatcc ggttgagcgt
     1981 cgccaggacc gcggcgggag acgcgtcacg ctcgacctgc agccgccagg cggtgtgggc
     2041 gacggccatg acgaggccgg cggccatccc gtgcccggaa gcgtcgccga agaggacggc
     2101 gagccgaccg tcggacaggg gctggaactc gtagatgtcc cccccgacgg tgttcgccac
     2161 gcggttgaag gccccgagct cccagccggg gacgacgggc ggggagaccg ggacgagctc
     2221 ggcctggagc tggcgcgcca ggacgagctc gtcccggaag cgaagcttgt cgacgagctc
     2281 gaggccgagg aggacgagga gcaggaagag gcagaggagg agggaaagga ccgtcccctt
     2341 cgcgtcggcg ggccagcccc ggccgacgag cttgatcgtc gagaggagga cgccgaggag
     2401 gacgaagagg acgacgagcc gccggaccgg cgagaggcgg cgcacgagcc cgtggaggag
     2461 ggcgctcacc ttcaggaggc cgcgccggaa cccgggcctc tcgcgctcga tcgcgtcgcg
     2521 gtgctcggag aggaggacct cccgcgcgtc ggcccactcg acggtgtaga ggtcggccac
     2581 gtcccgcgag tcgagcttct cga
//
LOCUS       DAOTEZ010000539         2596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_32189_length_2596_cov_6.123573, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000539 DAOTEZ010000000
VERSION     DAOTEZ010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32189_length_2596_cov_6.123573"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /gene="tssC"
                     /locus_tag="P5164_19070"
     CDS             <1..635
                     /gene="tssC"
                     /locus_tag="P5164_19070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014994539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HRY46039.1"
                     /translation="TRLTEAFAMYGWCVAIRGVEGGGLVQGLPVHTFQTDEGDVALKC
                     PTEIAITDRREKELSDLGFIPLVHCKNTDYAAFFGAQSAQKPKKYDLESANANARLST
                     MLPHIFAVSRFAHYLKAMMRDKVGSFMSRKDCEMFLNQWISNYVSPDDTASADTKARY
                     PLRDAKIEVAEIPGKPGAYRAVAFLRPHFQLDELQVSLRLVADLPASARK"
     gene            709..1191
                     /gene="tssE"
                     /locus_tag="P5164_19075"
     CDS             709..1191
                     /gene="tssE"
                     /locus_tag="P5164_19075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HRY46040.1"
                     /translation="MSRFDNEIRVHPSVLDRLIDYEPEYSREAPASRVKNLRILKQSV
                     RRDLEWLLNTRATAVVPPELKEVWSSIVVYGLPDISALSGRSPADKAKLRRAVERVLE
                     TFEPRLEGVVVTVDPEPGPGLSLKFRIDGRLKVDPAPEPVSFDTLLQIGSGEFRVKGE
                     "
     gene            1191..>2596
                     /gene="tssF"
                     /locus_tag="P5164_19080"
     CDS             1191..>2596
                     /gene="tssF"
                     /locus_tag="P5164_19080"
                     /inference="COORDINATES: protein motif:HMM:NF017733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HRY46041.1"
                     /translation="MRDELLGYYERELSFLRQMGAEFAEKYPKIAGRLQLEADTCEDP
                     HVERLIEAFAFLASRVHLKVDDEFPEITESLLNVLYPHLLAPIPSMSIVQFVLDPSQV
                     NLQSGQRIPRGSMLYSRSVAGTSCRFRTAYPVRIWPVEMRQARFELPIPGVGPEEGVR
                     TVLRLELKTFGGAPLRELREKVSESEEKPLDSLRFFLQGEGKLVYALHELIHNHLVAV
                     ELRPGGPRDVPSPVRLPKSSVAGVGFGRDEGLLPYTDRSFLGYRLLAEYFAFPEKFFF
                     FDVNGLAAAAREDFDDVLEILLYFDRDFPLERNVTAQTFRLNCTPVANLFRQVAEPIR
                     LTQLQPEYRVIPDVRRQDVMEVYSVEAVTSVAKGGDKVTSYEPFYSYRHGFEKGQAPA
                     FWYMSRRPSARKDDDGTDAYLSLVDLGFDPAEVDADTLTVETLCTNRDLPSRLPFEGG
                     EADFQLEGAGVFSGIRCLR"
BASE COUNT          391 a          934 c          861 g          410 t
ORIGIN      
        1 ggacccgcct cacggaggcg ttcgcgatgt acggctggtg cgtcgcgatc cggggcgtgg
       61 agggaggcgg cctcgtccag gggctcccgg tccacacgtt ccagaccgac gaaggggacg
      121 tcgccctgaa gtgcccgacg gagatcgcca tcaccgaccg gcgcgagaag gagctctcgg
      181 acctcgggtt catcccgctc gtccactgca agaacaccga ctacgccgcg ttcttcggcg
      241 cgcagtcggc gcagaagccg aagaagtacg acctcgagtc ggcgaacgcg aacgcccgcc
      301 tctcgacgat gctcccgcac atcttcgcgg tctcccgttt cgcgcactac ctgaaggcca
      361 tgatgcgcga caaggtcggc agcttcatgt cgcggaagga ctgcgagatg ttcctcaacc
      421 agtggatctc gaactacgtc tccccggacg acacggcgtc cgcggacacg aaggcccggt
      481 atccgctgcg cgacgcgaag atcgaggtcg ccgagatccc cggcaagccg ggcgcctacc
      541 gcgcggtggc gttcctcagg cctcacttcc agctcgacga gctgcaggtc tcgctccggc
      601 tcgtggccga cctgcccgcc tcggcgcgca agtagcccct cccggccgga cggcggcctc
      661 cgccgtccgg ccgatccgcc gcccgtccgc ccgaggagtc cgcccgagat gtcgcggttc
      721 gacaacgaga ttcgtgtcca cccgtcggtc ctcgaccggc tgatcgacta cgagcccgag
      781 tactcccgcg aggcaccggc ctcgcgcgtg aagaacctgc gcatcctcaa gcagtcggtg
      841 cggcgcgacc tcgagtggct cctgaacacg cgggcgacgg cggtcgtgcc gccggagctg
      901 aaggaggtct ggtcctccat cgtggtctac ggcctcccgg acatctcggc cctctcgggg
      961 cggagcccgg ccgacaaggc gaagctccgg cgcgcggtgg agcgggtcct cgagaccttc
     1021 gagccgcgcc tcgagggggt cgtcgtgacg gtcgacccgg agcccgggcc gggcctctcg
     1081 ctgaaattcc ggatcgacgg gaggctcaag gtcgaccccg cgccggagcc ggtctcgttc
     1141 gacaccctgc tccagatcgg gagcggggag ttccgcgtga agggggaatg atgcgcgacg
     1201 agctcctcgg atactacgaa cgcgagctct cgttcctccg ccagatgggg gccgagttcg
     1261 cggagaagta cccgaagatc gcgggtcgcc tgcagctcga ggccgacacg tgcgaggacc
     1321 cgcacgtcga gcggctcatc gaggcgttcg cgttcctcgc ctcgcgcgtc cacctgaagg
     1381 tcgacgacga gttccccgag atcaccgagt cgctcctcaa cgtcctctac ccgcacctcc
     1441 tcgcgccgat cccgtcgatg tcgatcgttc agttcgtcct cgatccctcg caggtgaacc
     1501 tgcagtcggg gcagcggatc ccgcgcgggt cgatgctcta ctcccggtcc gtcgcgggga
     1561 cgtcgtgccg tttccggacg gcgtacccgg tccgcatctg gccggtcgag atgcggcagg
     1621 cgcgcttcga gctgccgatc ccgggcgtcg gacccgagga gggcgtccgg accgtccttc
     1681 gcctcgagct gaaaacgttc ggcggcgcgc ccctgcgcga gctgagggag aaggtctccg
     1741 agtcggagga gaagccgctc gattcgctcc gcttcttcct ccagggggag gggaagctcg
     1801 tctacgcgct ccacgagctg atccacaacc acctcgtcgc ggtcgagctc cggcccggcg
     1861 ggccgcgcga cgtgccgtcg cccgtgcgcc tgccgaagag ctccgtcgcc ggcgtcggat
     1921 tcggccgcga cgagggcctt cttccctaca cggaccgctc cttcctgggg tatcgcctcc
     1981 tcgcggagta cttcgcgttc ccggagaagt tcttcttctt cgacgtgaac ggcctggcgg
     2041 cggcggcgcg agaggacttc gacgacgtcc tcgagatcct cctctacttc gaccgcgact
     2101 tcccgctcga gcggaacgtc acggcgcaga ccttccgcct caactgcacc cccgtcgcga
     2161 acctcttccg ccaggtcgcc gagccgatcc ggctgacgca gctccagccc gagtaccgcg
     2221 tgatccccga cgtccggcgc caggacgtca tggaggtcta ttccgtcgag gcggtcacga
     2281 gcgtcgcaaa ggggggcgac aaggtcacga gctacgagcc cttctactcg taccggcacg
     2341 gcttcgagaa gggccaggct cccgcgttct ggtacatgag ccgccggccg tcggcccgca
     2401 aggacgacga cgggaccgac gcctacctct cgctcgtcga cctcgggttc gacccggcgg
     2461 aggtcgacgc ggacacgctg acggtcgaga ccctctgcac gaaccgggac cttccctcgc
     2521 ggctcccgtt cgaagggggg gaggccgact tccagctgga gggggccgga gtcttctcgg
     2581 gcatccggtg cctgcg
//
LOCUS       DAOTEZ010000540         2595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_32214_length_2595_cov_6.191339, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000540 DAOTEZ010000000
VERSION     DAOTEZ010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32214_length_2595_cov_6.191339"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2566)
                     /locus_tag="P5164_19085"
     CDS             complement(<1..2566)
                     /locus_tag="P5164_19085"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HRY46042.1"
                     /translation="MVRTLARWLALVAAALAATGAPAQAPPATPAPADPSRGVDVTNA
                     APDPANALRAEALTLFGRADDDRTAAWVASGGGPDARRIVACPNLFGRIEVWTYLTHR
                     LLGPNARLIFFPEPGTGVGRYWTLLDGEAVLLAAKSEAGGPIGELADGAFGCADAALV
                     RDAVRSIRTRQGDTAGGLRERAALAAPISGPEQAAPAAGPAPLVLGGKRLSGKERKQA
                     LEALPERYQFFLRDVEPILTDVEETTFLRLGSDYQRDKFIEEFWKRRSVDADGQRVAF
                     KDIYELRLRVVKERYRGVHTDQGRIFIVNGPPDGMRRIDCQDIYNPIELWYYERLESL
                     RMSKVTLLFYQPFGTGDFRLWTPFDGQQAVLVGGVAGLAGISPGRRIDVTYCQEWREV
                     QGAMATAAGVFGTMGAQKMIDSLKSGPKPDVEGVDRILLMTTDVDPSAAKLPVQRVFR
                     FPEMVASKIRMEVSLLLERESLGTKALGEETFYDLDVVGEVVKGGKLVDNFRYRFDFP
                     ASTVKGAFLPLSIERFLYPGEYRLKVKVADANRNAAALVDEKVVVPEAADQSLSPEEK
                     AAREEAKAALARLVAEPNDRGVLTFLPLAREFATGVVRFETRASSDDVAYAEFFLDGR
                     KVVTDRRPPFEADFDLGELPRKRTVKVAGYAKDGRLLSEDEMILNEGREAFRVLITSP
                     EKGVPLEGPVRVVADLAVPQTKKLQQVEFFVNESRAATLYQPPFQQVVDVPRQKEIGF
                     LRVVATLVDGSVSEDVRYFNAPKYLSEVDVQAVELYTSVFRKGRPVTGLSRESFRVFE
                     DGAEQTIDGFEVVTNLPLALGLAVDTSGSMEETLPEAQKAANDFLQSVMTPRDRCFL"
BASE COUNT          359 a          909 c          943 g          384 t
ORIGIN      
        1 cgaggaagca gcggtcgcgg ggcgtcatga cgctctggag gaagtcgttc gcggctttct
       61 gcgcctcggg gagcgtctcc tccatcgagc ccgacgtgtc gaccgcgagc ccgagcgcga
      121 gggggaggtt cgtgacgacc tcgaagccgt cgatcgtctg ctccgcgccg tcctcgaaga
      181 cgcggaagct ctcccgcgac aggcccgtga ccgggcggcc cttgcggaag accgaggtgt
      241 agagctcgac ggcctggacg tcgacttccg aaaggtactt cggggcgttg aagtagcgca
      301 cgtcttcgct gacgctgccg tcgacgagcg tcgccacgac gcggaggaac ccgatctcct
      361 tctgccgcgg cacgtcgacg acctgctgga acgggggctg gtagagcgtc gccgcgcgcg
      421 actcgttgac gaagaactcc acctgctgga gcttcttcgt ctggggaacg gcgaggtcgg
      481 cgacgacgcg gaccggcccc tcgagcggca cgcccttctc cggggaggtg atcaggaccc
      541 ggaacgcctc gcgcccctcg ttgaggatca tctcgtcctc ggagaggagc cggccgtcct
      601 tcgcgtagcc ggcgaccttc accgtccgct tgcgcggcag ctccccgagg tcgaagtcgg
      661 cctcgaacgg cgggcggcgg tcggtgacga ccttccgccc gtcgaggaag aactcggcgt
      721 acgcgacgtc gtccgaggag gcgcgcgtct cgaagcggac gaccccggtc gcgaactcgc
      781 gcgcgagcgg gaggaaggtg aggacgccgc ggtcgttcgg ctcggcgacg agccgggcca
      841 gggccgcctt cgcctcctcg cgcgccgcct tctcctcggg cgagaggctc tggtcggccg
      901 cctcggggac gacgaccttc tcgtcgacga gcgcggccgc gttccggttc gcgtcggcga
      961 ccttgacctt cagccggtac tcgcccgggt agaggaaccg ctcgatgctg agcgggagga
     1021 acgccccctt caccgtcgag gcggggaagt cgaagcggta acggaagttg tcgacgagct
     1081 tccccccctt gacgacctcg cccacgacgt cgaggtcgta gaaggtctcc tcgccgaggg
     1141 ctttcgtgcc gagcgactcg cgctcgagga ggagcgagac ctccatccgg atcttgctcg
     1201 cgaccatctc gggaaagcgg aagaccctct ggaccgggag cttcgccgcg gacgggtcga
     1261 cgtcggtcgt catcaggagg atccggtcga ccccttccac gtccggcttg gggcccgact
     1321 tcagggagtc gatcatcttc tgcgcgccca tcgtgccgaa gacgccggcc gcggtcgcca
     1381 tggcgccctg gacctcgcgc cactcctggc agtaggtgac gtcgatgcgg cggccgggcg
     1441 agatgcccgc gaggccggcg acgcccccca ccaggacggc ctgctggccg tcgaacggcg
     1501 tccagagccg gaagtcgccc gttccgaacg gctggtagaa gaggagcgtg accttgctca
     1561 tccggagcga ctcgagccgc tcgtagtacc agagctcgat cgggttgtag atgtcctggc
     1621 agtcgatgcg ccgcatcccg tcgggcgggc cgttgacgat gaagatccgc ccctggtcgg
     1681 tgtgaacgcc gcggtacctc tccttcacga cccggaggcg cagctcgtag atgtccttga
     1741 acgcgacgcg ctgcccgtcc gcgtcgaccg agcgccgctt ccagaactcc tcgatgaact
     1801 tgtccctctg gtagtcggag ccgagccgca ggaacgtcgt ctcctcgacg tcggtcagga
     1861 tcggctcgac gtcccggagg aagaactggt agcgctcggg cagcgcctcg agcgcctgct
     1921 tccgctcctt tccggagagg cgcttgcctc cgaggacgag cggcgccggg ccggcggccg
     1981 gggccgcctg ctccggcccg gagatcgggg cggcgagcgc cgcgcgctcg cggagccccc
     2041 cggccgtgtc tccctggcgg gtccggatcg agcggacggc gtcgcgcacg agcgccgcgt
     2101 cggcgcatcc gaaggccccg tcggcgagct cgccgatcgg tccgccggcc tcggacttcg
     2161 ccgcgaggag caccgcctcc ccgtcgagga gggtccagta cctgccgacc ccggtccccg
     2221 gctccgggaa gaagatcagc cgggcgttcg ggccgagcag ccggtgcgtg aggtaggtcc
     2281 agacctcgat ccggccgaag aggttcggac aggcgacgat cctgcgggcg tcgggccctc
     2341 ccccggaggc gacccaggcc gcggtccggt cgtcgtcggc ccggccgaac agggtgagcg
     2401 cctccgcgcg gagggcgttg gccgggtcgg gcgccgcgtt cgtgacgtcg accccccggg
     2461 aggggtccgc cggggccggc gtcgcgggcg gagcctgggc cggcgcgccg gttgcggcga
     2521 gcgcggcggc cacgagggcg agccatcgtg cgagcgtccg gaccatcaga tgcgcctcct
     2581 gggcgtcccc tcctt
//
LOCUS       DAOTEZ010000541         2593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_32254_length_2593_cov_5.380221, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000541 DAOTEZ010000000
VERSION     DAOTEZ010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32254_length_2593_cov_5.380221"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="P5164_19090"
     CDS             complement(<1..203)
                     /locus_tag="P5164_19090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46043.1"
                     /translation="MIELADSHGHLTMVFPGPEARWPTREATDAEVGELVARARDAGV
                     TRVLVPATTLEDAPRAVEVAERF"
     gene            complement(225..1094)
                     /locus_tag="P5164_19095"
     CDS             complement(225..1094)
                     /locus_tag="P5164_19095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020041135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRY46044.1"
                     /translation="MAGRPVPPVPEDPAVRENLEKILASPSTLRAHEDLAWLARDELR
                     GTRLELELERAEAIISEAGVTGTIVVFGGTRVVEKPEALRRLAEAEAAAAAAPGDAEA
                     LRVVERARRVLAKARYYDEAREFARIASTTCLKNGRCDFVVVTGGGPGVMEAANRGAF
                     DVGATSLGFNIVLPHEQFPNPYITPGLCIYFHYFATRKMHFLIRAKALVAFPGGYGTL
                     DELFETLTLIQTRKMPPVPVVLFGREFWEKAIDLDYLVEEGTIAPEDARLVSYAETAA
                     EAWEAIARFHGIG"
     gene            complement(1094..>2593)
                     /gene="metG"
                     /locus_tag="P5164_19100"
     CDS             complement(1094..>2593)
                     /gene="metG"
                     /locus_tag="P5164_19100"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF020892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HRY46045.1"
                     /translation="SVPGIPFVFPETRLNEVRSFVAAGLKDLSVSRTAIEWGIPFPGH
                     PGHVVYVWLDALTNYLTALGLGSGDESLVARYWPAGDDRRVVHLVGKDILRFHAVYWP
                     AFLMSAGLPLPTQVVSHGFWLKDARKMSKSVGNVVRPDALVDAFGVDALRWHLISEMS
                     FGQDASFSDEAFLVRYNSDLANGLGNTLSRAVRMAADAFSGKTPSERCDDNEVKRAAE
                     TAVAAWDAAFRACRLHEAAEAVRVLLGAIDGYITAKEPWKKVKAEGVTPALHRIHHNA
                     LEGLRVAACLLAPIAPGAASEVLARLGAPKAAEAIGPSDLAWGGLPLGAPLAPAAPLF
                     PRADAKAYFAEPSEEKPVTEETKPAVPAVATVPTVPAEPAPAAAPAPAPAAAPAEAAR
                     ITVDDFFKADLRVAEIVAAEKVEKSKKLMKMKVRIGEEERTIVAGIATAYTGEQLVGR
                     KVVVVANLLPAKLMGIESNGMVLAASLPGTGEPSLLAVDPSVPSGTKVK"
BASE COUNT          368 a          902 c          962 g          361 t
ORIGIN      
        1 tcgaaccgct cggcgacctc gacggcgcgc ggggcgtcct cgagcgtcgt cgccgggacg
       61 aggacccgcg tcaccccggc gtcgcgtgcg cgtgcgacga gctccccgac ctcggcgtcg
      121 gtcgcctcac gcgtcggcca ccgggcctcg ggccccggga agaccatcgt caggtgcccg
      181 tgggagtcgg cgagctcgat cacgccgggg aggatagcgg accgtcaccc gatcccgtgg
      241 aaccgggcga tcgcctccca cgcttcggcc gccgtctcgg cgtacgagac gaggcgggcg
      301 tcctccgggg cgatcgtccc ctcctcgacg aggtagtcga ggtcgatcgc cttctcccag
      361 aactcgcgcc cgaagaggac gacgggaacg gggggcatct ttcgcgtctg gatcagcgtg
      421 agggtctcga agagctcgtc gagcgtcccg tagcctcccg gaaaggcgac gagcgccttc
      481 gcccggatca ggaaatgcat cttccgggtc gcgaaatagt ggaagtagat gcagaggccg
      541 ggcgtgatgt acggattcgg gaactgctcg tgcggcagga cgatgttgaa gccgaggctc
      601 gtggcaccga cgtcgaaggc gccccgattc gcggcctcca tgacacccgg cccgcccccg
      661 gtgacgacga cgaaatcgca gcggccgttc ttcaggcagg tcgtcgaggc gatcctcgcg
      721 aactcgcgcg cctcgtcgta gtaacgcgct ttcgcgagga cgcggcgggc ccgctcgacg
      781 acgcgaaggg cctctgcgtc gcccggcgcg gccgcggccg ccgcttcggc ctcggcgagg
      841 cggcggagcg cctcgggctt ctcgacgacg cgcgtgccgc cgaaaacgac gatcgtcccc
      901 gtgacgcccg cctccgagat gatcgcctcg gcccgctcga gctcgagctc gaggcgcgtg
      961 ccgcgcagct cgtcccgcgc gagccaggcc aggtcctcgt gcgcccgcag ggtgctcggc
     1021 gaggcgagga tcttctcgag gttctctcgg acggccgggt cctccggaac gggcgggacg
     1081 ggccggccgg ccactacttc accttcgtcc cggaggggac cgacgggtcg acggcgagga
     1141 gagaaggctc ccccgtcccc gggagcgagg ccgcgaggac catcccgttc gactcgatcc
     1201 ccatcagctt cgcggggagg aggttcgcca cgacgacgac cttccggccg acgagctgct
     1261 cccccgtata cgccgtggcg attccggcca cgatcgttcg ctcctcctcg ccgatccgca
     1321 ctttcatctt catcagcttc ttcgacttct cgaccttctc cgcggccacg atctcggcga
     1381 cgcggaggtc ggccttgaag aaatcgtcga cggtgatgcg cgcggcctcg gccggggcgg
     1441 cggcgggagc cggggcggga gcggccgccg gggccggctc ggcgggaacg gtcgggacgg
     1501 tggcgacggc gggaacggcg ggcttcgtct cttcggtcac gggcttctcc tcggaaggtt
     1561 cggcgaagta cgccttcgcg tcggcgcggg ggaagagcgg ggcggcgggc gcgagcgggg
     1621 caccgagcgg cagcccgccc caggcgaggt cggaggggcc gatcgcctcg gcggccttcg
     1681 gtgcgccgag ccgcgcgagg acctcggatg cagcccccgg cgcgatcggc gcgaggaggc
     1741 aggccgcgac gcgcagcccc tcgagcgcgt tgtggtggat ccggtgaagc gcgggggtga
     1801 ccccttccgc cttcaccttc ttccacggct ccttcgccgt gatgtagccg tcgatggcgc
     1861 cgaggaggac gcgcaccgcc tcggccgcct cgtggagccg gcaggcgcgg aaggcggcgt
     1921 cccacgccgc gacggccgtc tcggccgcac gcttcacctc gttgtcgtcg cagcgctccg
     1981 agggcgtctt ccccgagaag gcgtcggcgg ccatccgcac cgcgcgcgag agcgtgttcc
     2041 cgaggccgtt cgcgaggtcg ctgttgtagc ggacgaggaa ggcctcgtcc gagaacgacg
     2101 cgtcctggcc gaagctcatc tccgagatca ggtgccagcg gagcgcgtcg acgccgaacg
     2161 cgtcgacgag ggcatcgggg cggacgacgt tcccgaccga cttcgacatc ttccgcgcgt
     2221 ccttcagcca gaagccgtgg gagacgacct gcgtcgggag cgggaggccg gccgacatca
     2281 ggaacgcggg ccagtagacg gcgtggaagc ggaggatgtc ctttccgacg aggtggacga
     2341 cgcgccggtc gtccccggcc ggccagtagc gcgcgacgag cgactcgtcg ccgctcccga
     2401 ggccgagggc ggtgaggtag ttcgtcaggg cgtcgagcca gacgtagacg acgtgtcccg
     2461 ggtggcccgg gaaggggatc ccccactcga tcgcggtgcg cgagacggag aggtccttca
     2521 gccccgcggc gacgaacgag cggacctcgt tgagccgcgt ctcggggaag acgaacggga
     2581 tccccgggac gct
//
LOCUS       DAOTEZ010000542         2590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_32322_length_2590_cov_5.448521, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000542 DAOTEZ010000000
VERSION     DAOTEZ010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32322_length_2590_cov_5.448521"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..1022)
                     /locus_tag="P5164_19105"
     CDS             complement(60..1022)
                     /locus_tag="P5164_19105"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRY46046.1"
                     /translation="MSRPLRLDHAGALWHVTSRGNERREVFRDDADRREMLHVLGRVV
                     DLFGWQLHAWVLMGNHYHLLLGTPEATLSRGMRQLNGDYAGHFNRRHGRDGHVFQGRF
                     KAILVQREAHLLEVARYVVLNPVRAGLAASAADWPWSSFRATAGLEPAPEWLDTSFLL
                     DSFGSRRGPAAAKYREFVEAGTTGSYDPWSQVVGQIYLGSASWAESARAGQAAPARGR
                     EVPRRQREPVTPKPEEVVARFEEAFGLGLEELRHRPRLLSRERALLAAALRRHARATL
                     PVIAETFGVELSRASMLARRDGLDGATREQRRKVAAFEKLLAER"
     gene            complement(1061..1648)
                     /locus_tag="P5164_19110"
     CDS             complement(1061..1648)
                     /locus_tag="P5164_19110"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRY46047.1"
                     /translation="MPIRINRVYTRGGDKGETSLVGGQRIAKDSIRIESYGTVDELNA
                     ILGLARVANRDLPGASEADRARLDALLFRLQNELFNLGSDLATLPADRHPKQPVVEPR
                     HVAALEADIDGLNEGLPELTSFVLPGGGLVGAHLHQARTVCRRAERLVTTLARQEAIG
                     TLGLVYLNRLSDLLFVLSRWAARARSEPETLWRPE"
     gene            1700..2509
                     /locus_tag="P5164_19115"
     CDS             1700..2509
                     /locus_tag="P5164_19115"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HRY46048.1"
                     /translation="MRATDYHVHTARCGHAGGATRDYVLRAISRGLDEIAITDHVPLW
                     FLPGEDPEPGLAMSRAELPGYVEEVLALREEFRGRIDVLLGIEADYAEGHEEGLRALL
                     APYPWDVVLGSVHFVAGGWIDAPGAAARHEREGSANLWNEYYRLVAKAAGSGIYDVMT
                     HLDLPKKFGHRRPAECVAAEEAAVGSIRAAGVAVEVSSAGLRKPVGEEYPGPALLSKL
                     VVAGVPLVFSSDGHAPAEVAWGLEQVVAAAKAAGAIANATFFRRVRTLHPL"
BASE COUNT          351 a          892 c          996 g          351 t
ORIGIN      
        1 gctgagccgc cggagtcccg cgcgcatggc gcgacgacgc gcccgcgcca cgctcgtgtt
       61 catcgctcgg cgaggagctt ctcgaaggcg gcgactttgc ggcgctgctc gcgggtcgcc
      121 ccgtcgaggc cgtcgcgccg cgcgagcatc gacgccctcg agagctcgac tccgaacgtc
      181 tcggcgatga ccgggagcgt agcccgtgca tggcgccgga gggcggcggc gagcagggcg
      241 cgctcgcgcg agaggagacg cggccgatgg cggagctcct cgaggccgag gccgaacgcc
      301 tcttcgaagc gggcgacgac ctcctcgggc ttcggcgtca ccggctcgcg ctgccgccgc
      361 ggcacctcgc gtccccgggc cggggcggcc tgccccgcgc gcgccgactc ggcccacgag
      421 gcgctgccga ggtagatctg accgacgacc tggctccacg gatcgtacga gccggtcgtt
      481 cccgcctcga cgaactcgcg gtacttcgcg gcggccggac cgcgccgcga gccgaaggag
      541 tcgaggagga acgaggtgtc gagccactcg ggcgccggct cgaggcccgc cgtggcgcgg
      601 aagctgctcc acggccagtc ggccgcggag gccgcgaggc ccgcgcggac ggggttcagg
      661 acgacgtagc gcgccacctc gagaaggtgc gcctcgcgct ggacgaggat cgccttgaac
      721 cgcccctgga agacgtgccc gtcccgcccg tgccgccggt tgaagtggcc ggcgtagtcg
      781 ccgttgagct ggcgcatccc gcgcgagagc gtcgcctccg gcgtcccgag gaggaggtgg
      841 tagtggttcc ccatcaggac ccaggcgtgg agctgccagc cgaagaggtc cacgacccgg
      901 ccgaggacgt gcagcatctc gcgccggtcc gcgtcgtcgc ggaagacctc gcggcgctcg
      961 ttgccgcggg aggtgacgtg ccagagggcg ccggcgtggt cgaggcgaag ggggcggctc
     1021 atgccgtcag tatagtgtag atggtagacc tgaccccatt ctattccggg cgccagaggg
     1081 tctcgggctc ggagcgggcg cgggcggccc agcgggagag gacgaagagg aggtcggaga
     1141 ggcggttcaa gtagacgagg ccgagcgtgc cgatcgcctc ctgccgcgcg agcgtcgtga
     1201 cgagccgctc cgcgcgacgg cagacggtgc gcgcctggtg gaggtgggcg ccgacgaggc
     1261 ctccgcccgg gaggacgaag gacgtgagct ccgggagccc ctcgttcagc ccgtcgatgt
     1321 cggcctcgag cgcggcgacg tgccgcggct cgacgacggg ctgcttcggg tgacggtcgg
     1381 ccggaagcgt cgcgaggtcg gagccgaggt tgaagagctc gttctggagc cggaagagga
     1441 gcgcgtcgag gcgcgcgcgg tcggcctccg aggcgcccgg gagatcgcgg ttcgcgacgc
     1501 gcgccaggcc caggatggcg ttcagctcgt cgacggtgcc gtagctctcg atccggatcg
     1561 agtccttggc gatccgctgc ccgccgacga ggctcgtctc gcccttgtcg cccccccggg
     1621 tgtagacacg attgatccgg atcggcattc caccctcctc ccgctcccgc cctccggcgg
     1681 cctgctaccc tccgccgcga tgcgcgcgac ggattaccac gtccacaccg cccggtgcgg
     1741 gcacgcgggc ggcgcgaccc gagattacgt cctccgcgcg atctcgcggg ggctcgacga
     1801 gatcgcgatc accgaccacg tgccgctctg gttcctgccc ggcgaggacc cggagcccgg
     1861 cctcgccatg tcgcgcgcgg agctgcccgg gtacgtcgaa gaggtgctcg cgctgcgcga
     1921 ggagttccgc ggccggatcg acgtcctcct cgggatcgag gcggactacg cggaggggca
     1981 cgaggaaggt ctccgcgcgc tcctcgcccc gtacccgtgg gacgtcgtcc tcgggtcggt
     2041 ccacttcgtg gcgggagggt ggatcgacgc ccccggcgcc gccgcgcgcc acgagcgcga
     2101 ggggtcggcg aacctctgga acgagtacta caggctcgtg gcgaaggcag cgggaagcgg
     2161 gatctacgac gtgatgacgc acctcgacct gccgaagaag ttcgggcacc gccgcccggc
     2221 cgagtgcgtg gcggccgagg aggccgcggt cggatcgatc cgcgcggcgg gagtggcggt
     2281 ggaggtctcg agcgccggcc tccgcaagcc ggtcggcgag gaatacccgg ggcccgcgct
     2341 tctctcgaag ctcgtcgtcg ccggggttcc tctcgtcttc tcgtccgacg ggcacgctcc
     2401 ggccgaggtg gcgtggggcc tcgagcaggt cgtcgcggcg gcgaaggccg caggagcgat
     2461 cgcgaatgcg accttcttcc gccgcgtacg gacgctccac ccgctctgag ggtcggggag
     2521 ggggttcaac ggggaggagg ggggtcaggt ctaccatcta cactctgctc tcgagcagag
     2581 tgtagatggt
//
LOCUS       DAOTEZ010000543         2579 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_32567_length_2579_cov_3.093899, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000543 DAOTEZ010000000
VERSION     DAOTEZ010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32567_length_2579_cov_3.093899"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..300)
                     /locus_tag="P5164_19120"
     CDS             complement(73..300)
                     /locus_tag="P5164_19120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46049.1"
                     /translation="MSRHSLYQPRIQDRNVRRLYRLKVEHHERTGRKLPMTAIVNRIL
                     DDFFDAEDHDSAVASARSLPITADHAEEVLT"
     gene            complement(309..587)
                     /locus_tag="P5164_19125"
     CDS             complement(309..587)
                     /locus_tag="P5164_19125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46050.1"
                     /translation="MPFFPSDFLEPDGRYRNLLPADFVPILYLAADGGVLCPACVNDG
                     RPKHLDPNCPDDAQWTLVGYDLLYEGPPESCDHCGRDTATLYGDPDDG"
     gene            complement(592..789)
                     /locus_tag="P5164_19130"
     CDS             complement(592..789)
                     /locus_tag="P5164_19130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46051.1"
                     /translation="MRALPRIDCVRCGQDGAVERFSLGAYYSRLCDPCWDAATVRKEG
                     PEGFHPLDAGESLEAEDGEDW"
     gene            complement(789..1025)
                     /locus_tag="P5164_19135"
     CDS             complement(789..1025)
                     /locus_tag="P5164_19135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46052.1"
                     /translation="MPDATRRGCAECGSTADVRTYDDPETDAPVDLCEACARALCDDV
                     EDVTVGLVPADDDPDSTFADTCITAADYYREGDY"
     gene            complement(1038..1322)
                     /locus_tag="P5164_19140"
     CDS             complement(1038..1322)
                     /locus_tag="P5164_19140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46053.1"
                     /translation="MILDLSSLALSSAGAHLGTRPDAWLRASLDLAGIQHFVDLVAVL
                     VDRTGVQRAASRELDAMVRLHHLAYGADGPFATVTVRGQPFVLFVTPSSQ"
     gene            complement(1319..2143)
                     /locus_tag="P5164_19145"
     CDS             complement(1319..2143)
                     /locus_tag="P5164_19145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HRY46054.1"
                     /translation="MSPPVSPRWAELLAEAVSRPGLILEAYRRFHGYSLGNQLAASFQ
                     CLVRHIPPGPIATLQRWNELNRSVKPGEKALVLCMPVSVRVKEDDARSPVSTGNRPQL
                     KTIFVWKPRWFVLAQTEGAPLVDAVEIPTWDEGRALDALAVRRVPFALTNGNVQGYAE
                     GRAVAVSPLADLPAKTLFHELGHILLNHTGEPGTDRADLPKTLTEAEAEAVALLCLEA
                     LGLPGADYARGYIQSWYGAGQPLPEHSAQRIFKAADTILRAGRGDAQRQPAAREAA"
BASE COUNT          371 a          768 c         1071 g          369 t
ORIGIN      
        1 cggcgtcagt gaggaagccg aggtacgagc ggagcgcggc gctgagagaa gattcggagg
       61 ggtggatgcc gctcacgtca gcacttcctc ggcgtggtca gcggtgatcg ggagggagcg
      121 ggcggaggcg acggccgagt cgtggtcctc ggcgtcgaag aagtcgtcga ggatccggtt
      181 cacgatggcg gtcatcggga gcttccgtcc ggtgcgctcg tggtgctcga ccttgaggcg
      241 gtagaggcgg cggacgttgc ggtcctggat gcggggctgg tagaggctgt ggcgcgacac
      301 ggtgggcctc agccgtcgtc cgggtcgccg tagagggtcg ccgtatcccg gccgcagtgg
      361 tcgcagctct cgggcggtcc ctcgtagagg aggtcgtacc cgacgagcgt ccactgggcg
      421 tcgtcggggc agttggggtc gaggtgcttc ggccgcccgt cattcacgca cgcggggcag
      481 aggaccccgc cgtcggcggc gaggtacagg atcggaacga agtcggcggg aaggaggttg
      541 cggtagcggc cgtcaggctc gaggaagtcg ctgggaaaga agggcatggc ctcaccagtc
      601 ctccccgtcc tcggcctcga gcgactcgcc ggcgtcgagc gggtggaagc cctccggccc
      661 ctccttccgg acggtggccg cgtcccagca cgggtcgcag aggcgggagt agtaggcgcc
      721 gaggctgaac cgctcgacgg cgccgtcctg gccgcaccgg acgcagtcga tcctcgggag
      781 ggcgcgcatc agtagtcccc ctcgcggtag tagtcggcgg cggtgatgca ggtgtcggcg
      841 aacgtgctgt ccggatcgtc gtccgccggg acgaggccga cggtcacgtc ctcgacatcg
      901 tcgcagagcg cgcgggcgca ggcctcgcag aggtcgacgg gggcatcggt ctcggggtcg
      961 tcgtaggtgc ggacgtcggc ggtggagccg cactcggcac agccgcgcct cgtggcgtcg
     1021 ggcatggggg cctcctttca ctgggacgag ggagtaacga agaggacgaa gggctgtccg
     1081 cggaccgtga ccgtggcgaa ggggccatcg gccccgtatg cgaggtggtg gagccggacc
     1141 atggcgtcga gctcccgcga ggccgcgcgc tggaccccgg tacggtcgac gaggacggcg
     1201 acgaggtcga cgaagtgctg gatgccggcg aggtccagcg acgcgcggag ccaggcgtcg
     1261 ggtcgggtcc cgaggtgtgc gccggcggac gagagcgcga gggaggagag gtcgaggatc
     1321 atgcggcctc ccgggcggcc ggctgccgct gggcgtcacc gcgaccggcg cggaggatgg
     1381 tgtcggcggc cttgaagatc cgctgcgcgg agtgctccgg gaggggctgg ccggcgccgt
     1441 accaggactg gatgtagccg cgggcgtagt ccgcaccggg gagcccgagg gcctcgaggc
     1501 agaggagcgc gaccgcttcg gcctcggctt cggtgagcgt cttcgggagg tcggcgcgat
     1561 cggtgccggg ttcgccggta tggttcagga ggatgtgacc gagctcgtgg aagagggtct
     1621 tggccgggag gtccgcgagc gggctcacgg cgacggcgcg gccctcggcg tagccctgga
     1681 cgttgccgtt ggtgagggcg aacgggacgc gacgaacggc gagcgcgtcg agtgcgcgac
     1741 cctcgtccca ggtgggaatc tcgacggcgt cgacgagggg cgccccctcg gtctgggcga
     1801 ggacgaacca gcgcggcttc cagacgaaga tcgtcttgag ctgcggacgg tttccggtgg
     1861 agacgggact cctggcgtcg tcttccttca cgcggacgct gacgggcatg cagaggacga
     1921 gcgccttctc gcccggcttc accgagcggt tgagctcgtt ccagcgctgg agcgtggcga
     1981 tcgggccggg cgggatgtgg cggacgaggc actggaagct ggcggcgagt tggttcccga
     2041 ggctgtagcc gtggaagcgg cggtacgctt cgaggatgag gccggggcgg gagaccgcct
     2101 cggcgaggag ttctgcccag cgaggcgaga cgggtgggga cattgagacc tccttcgtag
     2161 ctggagcccg ggcgcacgcg cgcccgggac gcggtggcat ccggggcggg gcgaacgacg
     2221 ggagacggtg ggctcagtgc ttgaggtagg tcactggcac ttgcggctcg tcccagcagc
     2281 gccgacagtc accgcaggac ggcgggctcc ggagcgactt cgggcacgtg gtgctggatc
     2341 ggtcggtcgt gacggtgcta ccggccgcga gacccggaac ctccggtggg tcgccaccga
     2401 tgaagatggc gctcggtcgg accgtgacat tcgggagggc ggcgaggtgg cgtagcgaga
     2461 cgaggagcgg atcgctcccg gggcgacact gtcccgccat cgaccagctt cgcgtcggag
     2521 cccagaaggc gaccgccggg agggaccggg cgacctctcg ccacgcatcg acgtagcgc
//
LOCUS       DAOTEZ010000544         2574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_32661_length_2574_cov_4.680826, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000544 DAOTEZ010000000
VERSION     DAOTEZ010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32661_length_2574_cov_4.680826"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="P5164_19150"
     CDS             <1..776
                     /locus_tag="P5164_19150"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY46055.1"
                     /translation="LSTSDDRDPWYASWFGEEYLALYPNRDDLEAARQAQFAYGLLSP
                     YAAKGKGGILDLACGTGRHAVVLSRGGFPVTGVDLSAPLLSRARARRAEPDAGFVRAD
                     MRRLPFGSGSFGAVVNFFTSFGYFDDPADDLHVVAEVGRVLATGGAFLSDVFNSPRVL
                     ATLVSHEEKTVAGERVSIRRRFDAGTRRLEKEITMGSGSAARTFRERVRVYLRDELES
                     IHRSAGLRVVASFGDFDGSPFDVRRSPRLILLAVSGGSR"
     gene            773..2335
                     /gene="bshC"
                     /locus_tag="P5164_19155"
     CDS             773..2335
                     /gene="bshC"
                     /locus_tag="P5164_19155"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis BshC"
                     /protein_id="HRY46056.1"
                     /translation="MTIPLETLPGIPLLARGHATGAPDVSRFLGARASADSIAARAAE
                     VLHAFRPRAGADASLAALASGERAAVLTGQQVGLLTGPLLTAVKALATVKVAEALTAA
                     GTPLAPVFWCASEDHDLVEVTRVPLPGPEGPVEAGPDPEPLARNRRPVGELAVDVDVA
                     AILERARADAGALADGECVEALRAAHAGATYRSAFVTSLSWLLGEPALLFADAARAEE
                     KPFLVPLAVRLVREREVVRRRLETRAAELAAAGHPLQVVSEPGALPLFALVDGERLLL
                     REREGALELKGRDGERFAEEDVVARFEAGEWLPSFSALSRPLAASTLYPVAATVLGPA
                     ELAYWAQMLPLFEWAGLVPPILVPRPMAAPVTAAERRALGRLGLGLADLLAGVDALLA
                     RRGAVRAGELLDELVALRDGALSGLDALAPRLAAVEAGLGKNVAATRENVRFAFEKLA
                     DRARAAAGRADEAEARQLRRLAASLAPGGRPAERLYPPLPWLLRHGRAGLVGPLKEQL
                     RWDVAGLQEIDL"
     gene            2332..>2574
                     /locus_tag="P5164_19160"
     CDS             2332..>2574
                     /locus_tag="P5164_19160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRY46057.1"
                     /translation="MNETVEILAVGAHPDDVELGCGGTLALLARAGRSVGILDLTRGE
                     AGTRGTPETRAAEADEAAHLLGARFRDRLDLGDGNLR"
BASE COUNT          267 a          991 c          971 g          345 t
ORIGIN      
        1 ggttgagcac ctccgacgac cgggacccct ggtacgcgag ctggttcgga gaggagtacc
       61 tcgccctcta cccgaaccgg gacgacctgg aggcggcgcg ccaggcccag ttcgcctacg
      121 ggctcctctc gccgtacgcg gcgaagggga agggcgggat cctcgacctc gcctgcggca
      181 cggggcggca cgccgtcgtc ctctcgcggg gcggcttccc ggtgacgggc gtcgacctct
      241 cggcacccct cctgtctcgc gcgcgggcca ggcgggccga gccggacgcg ggcttcgtcc
      301 gcgccgacat gcgccgcctc ccgttcggga gcgggagctt cggcgccgtc gtcaacttct
      361 tcacctcgtt cggctatttc gacgacccgg ccgacgacct ccacgtcgtc gccgaggtgg
      421 ggcgggtgct ggcgacggga ggggccttcc tctccgacgt cttcaactct ccgcgcgtcc
      481 tcgcgacgct cgtctcgcac gaggagaaga cggtggcggg ggagagggtc tccatccggc
      541 gacggttcga cgcggggacg cggcggctcg agaaggagat caccatggga agcggctccg
      601 cggcgcgcac cttccgcgag agggtgcgcg tctacctgag ggacgagctg gagtcgatcc
      661 accggagcgc cgggctgcgg gtcgtcgcct cgttcggcga cttcgacggc tcccccttcg
      721 acgtgcggcg ctcgcctcgc ctgatcctcc tcgccgtctc cggagggagc cggtgacgat
      781 cccgctcgag acgcttcccg gaatcccgct cctcgcccgc ggtcacgcga ccggcgcacc
      841 cgacgtctcg cgattcctcg gggcgcgggc ctcggccgac tcgatcgcgg cccgggctgc
      901 ggaggtcctc cacgccttcc ggccccgggc cggcgccgac gcttcgctcg cggcgctcgc
      961 ctcgggggag cgggccgcgg tcctgaccgg acagcaggtc ggcctcctca ccgggccgct
     1021 cctgacggcg gtgaaggccc tcgcgaccgt gaaggtggcg gaggcgctga cggccgcggg
     1081 gacgccgctc gcgccggtct tctggtgcgc atccgaggac cacgacctcg tcgaggtgac
     1141 gcgcgtccct ctcccgggtc cggaaggtcc cgtcgaggcc ggccccgacc cagagccgct
     1201 cgcgcgcaac cgccggcccg tcggcgagct cgcggtcgac gtggacgtcg cggcgatcct
     1261 cgagcgggcg cgcgccgacg ccggggctct cgccgacggg gagtgcgtcg aggcgctgcg
     1321 cgcggcgcac gccggagcga cgtaccggag cgccttcgtg acgtccctct cgtggctcct
     1381 cggcgagccg gcgcttctct tcgcggacgc cgcgcgggcc gaggagaagc cgttcctcgt
     1441 ccctctcgcc gtccgcctcg tgcgggagcg ggaggtcgtg cgtcggcgtc tcgagacgcg
     1501 cgcggcggag ctggccgccg cgggccaccc gctccaggtg gtctcggagc ccggggcgct
     1561 cccgctcttc gcgctcgtcg acggggaacg gctcctcctc cgcgaacggg agggggctct
     1621 cgagctgaag ggccgggacg gcgagcgctt cgcggaggag gacgtcgtcg cccgcttcga
     1681 ggcgggcgaa tggctcccct ccttctccgc gctctcgcgc ccgctcgccg cgtcgacgct
     1741 ctatcccgtc gcggcgaccg tcctcgggcc ggcggagctc gcctactggg cccagatgct
     1801 gccgctcttc gagtgggccg ggctcgtgcc gccgatcctc gtgccgcggc cgatggccgc
     1861 tcccgtgacg gccgccgaac gccgggcgct cggccggctc ggcctcggcc tcgccgacct
     1921 cctcgccggc gtcgacgccc tcctcgcgag gcgcggcgcc gtccgggccg gggagctcct
     1981 cgacgagctc gtcgcgctac gcgacggggc gctctcgggg ctcgacgcgc tcgcgccgcg
     2041 gctcgccgcc gtcgaggcgg ggctcgggaa gaacgtcgcc gccacgcgcg agaacgtccg
     2101 gttcgcgttc gagaagctcg ccgaccgggc gcgggccgcc gccgggcgcg ccgacgaggc
     2161 cgaggcgcgc cagctccgcc gcctggcggc gtcgctcgcg ccgggcgggc gtcccgccga
     2221 gcgcctctat ccgccgcttc cgtggctcct ccggcacggc cgggccggcc tcgtcggccc
     2281 gctgaaggag cagctccgct gggacgtcgc ggggctgcag gagatcgacc tgtgaacgag
     2341 accgtggaga tcctggccgt cggggcccac ccggacgacg tcgagctggg ctgcgggggg
     2401 acgctcgccc tcctcgcccg cgccgggcgg agcgtcggga tcctcgacct gacgcgcggc
     2461 gaagcgggga cccgcggcac gcccgagacg cgggcggcgg aggccgacga agcggcgcac
     2521 ctcctcggcg cgcggttccg cgaccggctc gacctcggcg acgggaacct ccgg
//
LOCUS       DAOTEZ010000545         2570 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_32748_length_2570_cov_7.102187, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000545 DAOTEZ010000000
VERSION     DAOTEZ010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32748_length_2570_cov_7.102187"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="P5164_19165"
     CDS             complement(<1..773)
                     /locus_tag="P5164_19165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HRY46058.1"
                     /translation="MIRALLERISQKHRTMAWPDGPPPALPSRYRGLPAIDASRCPDG
                     CRACVDVCPTEAIAVVSGRPSLDLGRCLFCPECEAACPEKAISFTSDYRLAVRAREDL
                     SLGGAALPLARALEGKLLSLFGRSLKLRQVSAGGCNACEADVNVLGTIGWDLGRFGIQ
                     VVASPRHADGLLITGPVTRNMELALRKTWDAVPPPKIVIAVGACAISGGPFYDRPEQL
                     GGAAAVVPVDLFVPGCPPHPLTILDGLLRLLGRLEEEGRV"
     gene            complement(784..2307)
                     /locus_tag="P5164_19170"
     CDS             complement(784..2307)
                     /locus_tag="P5164_19170"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRY46059.1"
                     /translation="MSGAFARIRNGGALPLGKLPEADFDAFRGALLAELAAGRRLVAF
                     FAAPVDGAGADLWAVVAADGPGTLAIARTRLPDDRFPSLTPAAPQAHLFEREMAEQLG
                     LKPQGHPWLKPVRYRPSWRPGHDAWGRSEGEPILPAVGDFFRVEGTEVHEVAVGPVHA
                     GVIEPGHFRFQCHGEKVFHLEIALGFQHRGVEESLLGGPHRTTAARMETLAGDTTVGH
                     STAFAQALEALSGTAVPPRAQALRGIALELERLANHTGDLGALAGDVGFLPTASFCGR
                     LRGDFLNMTAVLCGNRFGRGLVRPGGTLVDADAALVADLSRRLEETMKDLRGAAELLF
                     ATATVTARFEGTGRVDPATAEALGLVGPAARACGVDQDVRRDHPSGVFRFVHVPVMTA
                     HDGDVNARAYVRWLEAQRSYEFVRAQLAALPEGIVRNAPKPLRPEHVAVSLVEGWRGE
                     VAHVAVTGVDGRFSRYKVVDPSFHNWTGLAMALRNEEISDFPLCNKSFNLSYCGHDL"
     gene            complement(2304..>2570)
                     /locus_tag="P5164_19175"
     CDS             complement(2304..>2570)
                     /locus_tag="P5164_19175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HRY46060.1"
                     /translation="AIGGGHAVAGTLLLVLLGIVFIGMGSTVLSAVQGRPGTASAGTP
                     YKDDLARTAPILVAAALVLLLGLWLPEPLVSLLERAAEGLEARP"
BASE COUNT          361 a          873 c         1048 g          288 t
ORIGIN      
        1 acgcgcccct cctcctcgag ccgtccgagg aggcggagga ggccgtcgag gatcgtgagc
       61 gggtggggcg ggcagcccgg gacgaagagg tcgaccggga cgacggcggc cgcgccgccg
      121 agctgctcgg gacggtcgta gaacgggccg cccgagatcg cgcaggcgcc gacggcgatg
      181 acgatcttcg gcggcgggac ggcgtcccac gtctttcgga gcgcgagctc catgttcctc
      241 gtgacggggc cggtgatcag gaggccgtcg gcgtgccgcg gcgaggcgac gacctggatc
      301 ccgaagcggc cgaggtccca gccgatcgtc ccgaggacgt tcacgtcggc ctcgcaggcg
      361 ttgcagccgc cggcgctcac ctgccggagc ttcagcgacc gtccgaagag cgagaggagt
      421 ttcccctcga gcgcccgcgc cagcgggagc gccgcgccgc cgagcgagag gtcctcgcgc
      481 gcgcggacgg cgaggcggta gtcggaggtg aaggagatcg ccttctcggg gcaggcggcc
      541 tcgcactcgg ggcagaagag gcagcggccg aggtcgagcg acggccttcc ggagacgacc
      601 gcgatcgcct cggtggggca gacgtcgacg caggcgcggc agccgtcggg gcagcgggac
      661 gcgtcgatcg cggggaggcc gcggtagcgg gaggggagag ccggcggcgg gccgtcgggc
      721 caggccatcg tccggtgctt ctgggagatc cgctcgagga gggccctgat catcggctgt
      781 ccctcacagg tcgtgcccgc agtaggagag gttgaagctc ttgttgcaga gcgggaaatc
      841 ggagatctcc tcgttccgga gggccatcgc gaggcccgtc cagttgtgga acgaggggtc
      901 gacgaccttg tagcgcgaga agcggccgtc gacgcccgtc acggcgacgt gggcgacctc
      961 gccccgccaa ccctcgacga gggagacggc gacgtgctcg ggccggagcg gcttcggggc
     1021 gttccgcacg atcccctccg ggagcgccgc gagctgcgcc cgaacgaact cgtacgagcg
     1081 ctgcgcctcg agccagcgga cgtaggcgcg ggcgttcacg tcgccgtcgt gcgccgtcat
     1141 gacggggacg tggacgaagc ggaagacccc ggaggggtgg tcgcgccgca cgtcctggtc
     1201 gacgccgcag gcgcgcgcgg ccggcccgac gaggccgagc gcctcggccg tcgccgggtc
     1261 gacgcgcccg gtcccctcga agcgggccgt caccgtggcg gtcgcgaaga ggagctccgc
     1321 ggcgccgcgc aggtccttca tcgtctcctc gagtcggcgc gagaggtccg cgacgagcgc
     1381 ggcgtcggcg tcgacgagcg tcccgccggg gcggacgagg ccgcgcccga agcggttgcc
     1441 gcagaggacg gccgtcatgt tcaggaagtc gccgcgcagg cggccgcaga acgaggcggt
     1501 cggcaggaag ccgacgtccc ccgcgagggc gccgaggtcg ccggtgtggt tcgcgagccg
     1561 ctcgagctcg agggcgatgc cgcgcagcgc ctgcgcgcgc ggcgggacgg cggtccccga
     1621 gagggcttcg agcgcctgcg cgaaggcggt ggaatgcccg acggtcgtgt cgcccgcgag
     1681 cgtctccatc cgggcggccg tcgtccggtg gggcccgccg agaagcgact cctcgactcc
     1741 gcggtgctgg aatccgaggg cgatctcgag gtggaagacc ttctcgccgt ggcactggaa
     1801 ccggaagtgc cccggctcga tgacgccggc gtggaccggg ccgacggcga cctcgtggac
     1861 ctcggtgccc tcgacgcgga agaagtcgcc gaccgcgggg aggatcggct ctccctccga
     1921 acggccccag gcgtcgtgcc ccggccgcca cgaggggcgg tagcggaccg gcttcagcca
     1981 cgggtgcccc tgcggcttca ggccgagctg ctcggccatc tcccgctcga agaggtgcgc
     2041 ctgcggcgcg gcaggggtga gcgacgggaa gcggtcgtcc ggaaggcgcg tccgcgcgat
     2101 cgcgagcgtc ccggggccgt cggcggcgac gaccgcccag aggtcggcgc cggcaccgtc
     2161 gacgggagcg gcgaagaagg cgacgaggcg ccgcccggcc gcgagctcgg cgaggagcgc
     2221 gccgcggaag gcgtcgaagt ccgcttccgg gagcttgccg agcgggagag cgcccccgtt
     2281 gcggatgcgg gcgaaggcgc cgctcacggc cgcgcctcca gcccctcggc ggccctttcg
     2341 aggagggaga cgagcggctc ggggagccag agcccgagga ggaggacgag cgcggcggcg
     2401 acgagaatcg gggccgtccg cgcgaggtcg tccttgtacg gcgtcccggc cgaggcggtg
     2461 ccgggccgcc cctggacggc cgagaggacc gtcgagccca tgccgatgaa gacgatgccg
     2521 aggaggacga ggaggagcgt cccggcgacg gcgtggccgc cgccgatcgc
//
LOCUS       DAOTEZ010000546         2569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_32774_length_2569_cov_6.188146, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000546 DAOTEZ010000000
VERSION     DAOTEZ010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32774_length_2569_cov_6.188146"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1613
                     /gene="kdpA"
                     /locus_tag="P5164_19180"
     CDS             <1..1613
                     /gene="kdpA"
                     /locus_tag="P5164_19180"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HRY46061.1"
                     /translation="ERTFLDPVLLPVERALYRLGGVDPKKEQDWRAYASSMLVFSVLG
                     ILGVTAIERLQHLLPLNPDRLPAVPPALAWNTAVSFVTNTNWQAYAGEATMSHLTQMA
                     ALAVQNFLSAATGIAIAVAVIRGIARTESRTIGSFWVDLTRATLWVLLPLSLLAAVVL
                     TSQGVVQSFSPSAKVTTLDGATQTVPLGPVASQEAIKELGTNGGGFFNANSSHPYENP
                     TPATDLLEMLLIFAIPAGLTYTLGSMTGKTRHGWAVFWAMGLLFLVGAGAAAWAETHG
                     NPIHHAAGVDPSAGNMEGKEVRFGAGDTALFATVTTAASCGAVNGMHDSFTPLGGLVP
                     LVNIQLGEVVFGGVGAGLYGAFVFIVLSVFIAGLMVGRTPEYLGKKIEGGEVKLAMLS
                     VLVLTFCILVGTAAAVTTKAGLSSLSNAGPHGLSQALYAFTSAAGNNGSALAGLNANT
                     DFYNVLLACAMFFGRFLMIVPILAMAGALAAKKKLADSAGTFPVDGPIFTLLLAGVIV
                     VVGALTYFPVLSLGPVVEQLLMAAGRTF"
     gene            1622..>2569
                     /locus_tag="P5164_19185"
     CDS             1622..>2569
                     /locus_tag="P5164_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRY46062.1"
                     /translation="MTKHAESRLLDPSLLGRAAADSFRKLHPRHMARNPVMLVVEAGA
                     VLATLPLVGLGTRGSLSFQLQVVLWLWATVLFANFAEAVAEGRGKAQADALRKTKTDA
                     VARRLVGAGRREEQVPASSLGKGDLVVVETGQLIPGDGTVVEGIASVDESAITGESAP
                     VIREAGGDRSAVTGGTRVLSDRIVVEITSEPGSTFLDRMIRLVEGAERQKTPNEIALD
                     ILLSGLTIVFLLATVTLRPFALYSGSGLSPTVLVALLVCLIPTTIGGLLSAIGIAGMD
                     RVLRHNVLAMSGRAVEASGDVNVLLLDKTGTITLGNRQAT"
BASE COUNT          341 a          982 c          841 g          405 t
ORIGIN      
        1 gcgagcggac gttcctcgac cccgtcctcc tcccggtgga gagggccctc taccgtctcg
       61 gcggcgtcga cccgaagaag gagcaggact ggagggccta cgcctcctcg atgctcgtct
      121 tcagcgtcct cggcatcctc ggcgtcaccg cgatcgagcg cctccagcac ctcctgccgc
      181 tgaaccccga ccgcctcccg gccgtcccgc ccgccctcgc ctggaacacg gcggtctcgt
      241 tcgtgacgaa cacgaactgg caggcctacg cgggcgaggc gacgatgtcg cacctgaccc
      301 agatggccgc gctcgcggtc cagaacttcc tctcggccgc gaccgggatc gcgatcgccg
      361 tggccgtcat ccgggggatc gcgcgcaccg aatcccggac gatcggcagc ttctgggtcg
      421 acctgacgcg ggcgacgctc tgggtgctcc tcccgctctc gctccttgcg gcggtcgtcc
      481 tgacgtcgca gggggtcgtc cagagcttct ctccttccgc gaaggtgacg acgctcgacg
      541 gggcgacgca gacggtcccg ctcgggccgg tcgcctcgca ggaggcgatc aaggagctcg
      601 ggacgaacgg aggcggcttc ttcaacgcga actcctccca cccgtacgag aacccgacgc
      661 ccgcgacgga cctcctcgag atgctcctca tcttcgcaat cccggcaggt ctgacgtaca
      721 cgctcgggtc gatgaccggc aagacgcgcc acggctgggc cgtcttctgg gcgatggggc
      781 tcctcttcct cgtcggggca ggggccgccg cgtgggccga gacccacggc aacccgatcc
      841 accacgccgc gggcgtcgac ccgagcgccg ggaacatgga ggggaaggag gtccgcttcg
      901 gcgcgggcga caccgccctc ttcgcgacgg tcacgacggc cgcctcctgc ggcgccgtga
      961 acgggatgca cgactcgttc acgccgctcg ggggcctcgt cccgctcgtg aacatccagc
     1021 tcggcgaggt ggtcttcggc ggcgtcggcg cggggcttta cggggccttc gtcttcatcg
     1081 tcctctcggt cttcatcgcg gggctgatgg tcggccggac ccccgagtac ctcggcaaga
     1141 agatcgaagg cggcgaggtg aagctcgcga tgctctcggt cctcgtcctc accttctgca
     1201 tcctcgtcgg tacggccgcg gccgtgacga cgaaggcggg cctctcgagc ctttcgaatg
     1261 ccgggcccca cggcctctcg caggcgctct acgccttcac gagcgcggcc gggaacaacg
     1321 gcagcgccct cgccggcctg aacgcgaaca ccgacttcta caacgtgctg ctggcctgcg
     1381 cgatgttctt cgggcggttc ctgatgatcg tcccgatcct cgcgatggcc ggagcgctcg
     1441 ccgcgaagaa gaagctcgcc gactccgcgg ggacgttccc cgtcgacggc ccgatcttca
     1501 cgctcctcct cgccggtgtc atcgtcgtcg tcggcgcgct cacctacttc cccgtcctct
     1561 ccctcggccc ggtcgtcgag cagctgctga tggccgcggg ccggaccttc tgaggccccc
     1621 cgtgacgaag cacgccgagt cccggctcct cgacccgtcc ctcctgggcc gcgccgcggc
     1681 cgactccttc cgcaagctcc acccccgcca catggcgagg aacccggtga tgctcgtcgt
     1741 cgaggcgggc gccgtcctcg cgacgctccc gctcgtcggc ctcggcacgc gcggctcgct
     1801 ctcgttccag ctccaggtcg tcctctggct ctgggccacc gtcctcttcg cgaacttcgc
     1861 cgaggcggtc gccgaagggc gggggaaggc gcaggccgac gcgctccgga agacgaagac
     1921 cgacgccgtc gcccgccgcc tcgtcggcgc cgggcggcgc gaggagcagg tcccggcctc
     1981 gagcctcggc aagggcgacc tcgtcgtcgt cgagacgggc cagctgatcc ccggcgacgg
     2041 gaccgtcgtc gaggggatcg cctcggtcga cgaatcggcc atcacgggcg aatcggcccc
     2101 ggtcatccgt gaggcgggcg gcgaccgctc cgccgtgacg ggagggacgc gggtcctctc
     2161 cgaccggatc gtcgtcgaga tcacctccga gcccggctcg acgttcctcg accggatgat
     2221 ccggctcgtc gagggggccg agaggcagaa gacgccgaac gagatcgccc tcgacatcct
     2281 cctctcgggg ctgacgatcg tcttcctcct cgcgacggtc acgctccgcc cgttcgcgct
     2341 ctactcgggg agcggcctgt cgccgaccgt cctcgtcgcc ctcctcgtct gcctcatccc
     2401 gacgacgatc ggcggcctcc tctcggcgat cggcatcgcc ggcatggacc gggtcctccg
     2461 tcacaacgtc ctcgcgatgt cggggcgcgc cgtcgaggcc tcgggcgacg tcaacgtcct
     2521 cctcctcgac aagaccggga cgatcaccct cggcaaccgc caggcgacg
//
LOCUS       DAOTEZ010000547         2553 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_33096_length_2553_cov_5.144516, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000547 DAOTEZ010000000
VERSION     DAOTEZ010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33096_length_2553_cov_5.144516"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..2410)
                     /locus_tag="P5164_19190"
     CDS             complement(50..2410)
                     /locus_tag="P5164_19190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46063.1"
                     /translation="MALRGRAREALAGAAPAAILVAAFATLFFDVLVRGQTFLGSDPA
                     RHHFPLLSLVRPLWSLEGGLPLWNPLLAAGQPWAANPEQAAFHPLTWLFLVFPPTLAF
                     RLQVLVPVAAAMAGAAFLGRALRLPPAAAGALALAWGAGGAFLSSTHYFPALFAGAAL
                     PWVLGFAARVARGGGPSAVAGLAVAEAFLCATGEPTQMLLSGPLAILAAWHALRAYAE
                     ADAPPRAWRPVAARAAGLAAGLALGALIAAPTLLPGAFHARKTVRADGLPEHVASYWS
                     ATPWRAGELLVPSVLGRESDPAGGEAARDTWRDRLYPGRGEPYLASVYPGLVATLLAA
                     FAWGRGAPGARAWGGAGLLAALVAAGRHGPLWAHLRELPLLDGIRYPEKLLLAAASGV
                     AVSAALGLAALVEAPARTRRALGLSAAALALLLLAGWGLGTRSDGAYPAQLLAEQALP
                     QAALAAAAAALLLLVPETGRGRTALVGLLLVLLGADLVLRGRPVLRTGSPRHLVELPP
                     QFEALRRNPPRGYLVHAAAWTPELWKVEAIGEPPAMARFGIASALDYDFGMTELAWSA
                     RATETIFSTIARAPDAAGPVLRRRSVAGVIRFRPGAPRSRKEADRIPRAELLELLALR
                     DPLPFAFLATEARPVRDAAEWSRTVVALGEGAATTACVDAAYAGALPSRLSGGGAAVI
                     ERRPGRIRIEVAAVGPEPALLAVNQTWDPGWRATVDGRPVRLLRTDLSLSGLVVPPGR
                     HEVVLRYEDPAVTAGLALGACGLAACVALALRGRRRLRTGAVSSEG"
BASE COUNT          373 a          945 c         1007 g          228 t
ORIGIN      
        1 tcctctccga accagctcgc gtaccagggg tcccggtcgt cggaggtgct caaccttccg
       61 atgatacggc ccccgttcgg agcctccgcc gcccccgcag ggccagcgcg acgcaggcgg
      121 cgaggccgca ggcgccgagc gcgaggccgg ccgttacggc cgggtcctcg taccggagga
      181 cgacctcgtg ccgacccgga gggacgacga ggcccgagag cgagaggtcc gtccgcagga
      241 gccgcaccgg ccggccgtcg acggtcgccc gccagccggg gtcccacgtc tggttcaccg
      301 cgagaagggc cggctccggc cccacggcgg cgacctcgat ccggatccgc cccggccggc
      361 gctcgatcac ggcggctccg ccccccgaga ggcgcgacgg cagcgccccg gcgtaagccg
      421 cgtcgacgca cgcggtcgtc gcagccccct cgcccagggc gacgacggtc cggctccact
      481 ccgccgcgtc gcgcacgggg cgggcctccg tcgcgaggaa cgcgaagggg agcgggtcgc
      541 ggagcgcgag gagctcgagg agctccgccc gcgggatgcg gtcggcctct ttccgcgaac
      601 gcggagcgcc gggccggaag cggatgacgc ccgcgaccga tctccgccgc aggaccgggc
      661 ccgccgcgtc gggcgcgcgg gcgatcgtgg agaagatcgt ctccgtcgcg cgcgcgctcc
      721 aggcgagctc cgtcattccg aagtcgtagt cgagcgccga cgcgatcccg aagcgcgcca
      781 tcgcgggcgg ctcgccgatc gcctcgacct tccagagctc cggcgtccag gcggccgcgt
      841 ggacgaggta gccgcgcggc ggattccggc gaagcgcctc gaactgaggc gggagctcca
      901 cgaggtgccg gggggatccc gtccggagaa ccggccgccc acgaaggacg aggtcggcgc
      961 cgagcagcac gaggaggagg ccgacgagcg ccgtgcgtcc tcttccggtc tccgggacca
     1021 ggagaagaag cgcggcggcc gcggccgcga gcgcggcctg cgggagcgcc tgctccgcga
     1081 ggagctgcgc cgggtacgct ccatcggacc gcgtgccgag cccccatccg gccaggagca
     1141 ggagggcgag ggcggccgcg gacaggccga gggcgcgccg cgtgcgcgcc ggcgcctcga
     1201 ccagggcggc gaggccgagc gccgccgaga ccgccacccc ggacgcggcg gcgaggagga
     1261 gcttctccgg gtaacggatg ccatccagca ggggaagctc gcgaagatgc gcccagagcg
     1321 gaccgtggcg gcccgcggcc accagcgcgg cgagaaggcc ggcgccgccc caggcccggg
     1381 ctccgggggc gccgcgcccc caggcgaatg cggcgaggag cgtcgcgacg aggcccgggt
     1441 agacggatgc gaggtacggc tcgccgcgcc ccgggtagag ccggtcccgc cacgtgtcgc
     1501 gagcagcttc gccgccggcc ggatcggatt cccggccgag gacgctcggg acgaggagct
     1561 cccccgctct ccagggcgtc gccgaccagt aggacgccac gtgctccggg agcccgtcgg
     1621 cccggaccgt cttgcgtgcg tgaaacgcgc ccgggagaag cgtcggcgcc gcgatcaggg
     1681 cgccgagggc gaggccggcc gcgaggcccg ccgccctcgc cgcgacgggc cgccaggcac
     1741 ggggcggcgc gtcggcttcg gcgtacgctc ggagcgcgtg ccacgcggcg aggatcgcga
     1801 gcggtccgga gaggagcatc tgggtcggct cgccggtcgc acagaggaag gcctccgcga
     1861 cggcgaggcc ggcgacggca gacggccccc cgccgcgcgc cacccgggcg gcgaaaccga
     1921 ggacccaggg gagggctgcg cccgcgaaga gggccgggaa gtagtgggtc gacgagagga
     1981 acgcccctcc cgctccccag gccagggcga gggccccggc cgctgcgggc ggaagccgca
     2041 gggctcgccc cagaaaggcc gcacccgcca tcgcggccgc tacgggaacg aggacctgca
     2101 ggcggaaggc gagggtgggc gggaagacga ggaagagcca cgtcaacggg tggaaggccg
     2161 cctgctcggg gttcgcggcc cacggctgcc ccgcagcgag aagcgggttc cagaggggga
     2221 gccccccctc gaggctccag agcggccgga cgaggctcag caggggaaag tggtggcggg
     2281 ccgggtcact tccgaggaac gtctgccccc ggacgaggac gtcgaagaag agcgtcgcga
     2341 aggcggcgac gagaatcgcg gccggagccg ctcccgcgag agcctccctc gcccgcccgc
     2401 gaagcgccat ccctcgatgc tacccgaggc gcggaacgcg ccggctcggg cctcagaacc
     2461 cccagagcgc gagctcgtcg atgacggcgg ccaggcccgg gtgcttcacg agctcctcgt
     2521 ggagccagcc gtgccgctcc ttcaccgcgg cga
//
LOCUS       DAOTEZ010000548         2535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_33449_length_2535_cov_4.635081, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000548 DAOTEZ010000000
VERSION     DAOTEZ010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33449_length_2535_cov_4.635081"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="P5164_19195"
     CDS             <1..901
                     /locus_tag="P5164_19195"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRY46064.1"
                     /translation="LGLTLAGEVAAAAAGKPWAELVKAGILGPLGLTGTEVEHLDRWR
                     GNRLATGYTAPRRDGTREKVPPYEVRGIGPAAGMTSTVEDLGRFASWQLRLLGTGKTE
                     LLDVDSLREMQRVQFMDPAWKLSWGLGFQVWRSGEKTFVGHNGYCPGYQSQLLVQTEE
                     KLATVFMTNTNGIDAQRYAQVAYDVVAPAAKKALEDPKGAKAHDPAFVRYAGRYDNWL
                     AGEANVFPWDDGLALLPTPSEKPLDALVKLKPAGEHRFRRLRDDGTLGEEIRFEVDAT
                     GRAVRLWRFSNAMERAADPLPAK"
     gene            complement(938..1534)
                     /locus_tag="P5164_19200"
     CDS             complement(938..1534)
                     /locus_tag="P5164_19200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRY46065.1"
                     /translation="MSTSRSPKKEATYADLLALPENVIGEIVDGELIASPRPAAPHAR
                     ASSVLAGDLGPPFDRGRGGPGGWWIVFEPEVHLGRQVLVPDLAGWRRERMPAFPTDAF
                     FTLAPDWVCEVLSPSTQRLDRVRKLAVYLEEGVTWAWLVDPLARTLEVFESRGSRWTL
                     AGAWEGDAVVRAVPFDAVEIEIGGWWVPGAEPAGEPGS"
     gene            complement(1590..>2535)
                     /locus_tag="P5164_19205"
     CDS             complement(1590..>2535)
                     /locus_tag="P5164_19205"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY46066.1"
                     /translation="KREILERECFGLVEFVEPGHDFSVVGGMDEVKKDLLVLADNIRE
                     GRRSRVPMGMLFTGPMGTGKTFVAEAFAKECGLPTIKLKNFRSKWVGATEGNLERILT
                     VIKAIGQVVVIIDEGDRAFGNTDGEGDGGTSSRVIARIKEFMSDTQNRGRILFLVMTN
                     RPDKLDVDLKRAGRLDRKIPFLYSQTPEEVEAVAKALVKKNRVKSDVDLSTIRDTFSA
                     KLVGYSNADVEAVVLMANDDAAREAGGDAPVTAEHFVTAATDYFPSRDAELLEYMELL
                     AVFEASSRRLLPPKYAHMTPEELDVRLRLLRATVGTRR"
BASE COUNT          361 a          924 c          896 g          354 t
ORIGIN      
        1 cctcggcctg acgctcgcgg gggaggtcgc cgccgccgcg gccgggaagc cctgggccga
       61 gctcgtgaag gccgggatcc tgggtccgct cgggctgacg gggaccgagg tcgagcacct
      121 cgatcgctgg cgcggaaacc gcctcgcgac gggctatacc gcgccccgca gggacgggac
      181 gcgggagaag gtgccgccct acgaggtccg cggcatcggt ccggccgccg ggatgacctc
      241 gacggtcgag gacctcggcc ggttcgcctc ctggcagctc cgcctcctcg gcaccgggaa
      301 gacggagctc ctcgacgtcg actctctgcg cgagatgcag cgggtccagt tcatggaccc
      361 cgcctggaag ctgagctggg ggctcggctt ccaggtctgg cggagcggcg agaagacgtt
      421 cgtcgggcac aacggctact gccccggcta ccagagccag ctcctcgtgc agacggagga
      481 gaagctcgcg accgtcttca tgacgaacac gaacgggatc gacgcgcagc gctacgcgca
      541 ggtcgcgtac gacgtcgtcg ccccggccgc gaagaaggcg ctggaggacc cgaagggggc
      601 gaaggcgcac gacccggcct tcgtccgcta cgcgggccgg tacgacaact ggctcgcggg
      661 cgaggcgaac gtcttcccgt gggacgacgg cctcgcgctc ctcccgaccc cctccgagaa
      721 gccgctcgac gccctcgtga agctgaagcc cgccggcgag catcgcttcc ggcgcctccg
      781 cgacgacggc acgctcggcg aggagatccg tttcgaggtg gacgcgacgg gccgcgccgt
      841 ccgactctgg cgcttcagca acgcgatgga gcgggccgcc gacccgctgc cggcgaagta
      901 gccggggagc gccgcgacgg ccggcggacc ggccggatca gctccccggc tcgcccgccg
      961 gctcggcgcc gggcacccac cagccgccga tctcgatctc caccgcgtcg aacgggacgg
     1021 cgcggacgac ggcgtccccc tcccaggccc ccgcgagcgt ccagcgactc ccgcggctct
     1081 cgaagacctc gagagtccgc gcgagcgggt cgacgagcca cgcccaggtc accccttcct
     1141 cgaggtagac cgcgagcttg cgcacccggt cgaggcgctg cgtggaaggg gagaggacct
     1201 cgcagaccca gtcgggggcg agggtgaaga acgcgtccgt cgggaaggcg ggcatccgct
     1261 cccgccgcca gccggcgagg tcggggacga ggacctgccg cccgaggtgg acctccggct
     1321 cgaagacgat ccaccagccg cccggcccgc cccgtccccg gtcgaacggc ggcccgaggt
     1381 cccccgcgag aaccgaggag gcccgcgcgt gcggcgccgc cgggcgggga gaggcgatca
     1441 gctccccgtc gacgatctcg ccgatcacgt tctccggcag ggcgaggagg tcggcgtacg
     1501 tcgcctcctt cttcgggctc cggctcgtgg acacggggga agtctagcgc ccccgccgat
     1561 ctcccccgcg tccggcacgc cggcgcggtc taccttctcg ttccgaccgt tgcccggagc
     1621 agccggagcc gcacgtcgag ctcctcgggc gtcatgtggg cgtacttggg cgggaggagg
     1681 cggcggctgg acgcctcgaa gacggcgagc agctccatgt actcgaggag ctcggcgtcg
     1741 cgcgagggga agtagtcggt cgccgccgtc acgaagtgct ccgcggtgac gggcgcgtcg
     1801 ccccccgcct cgcgggccgc gtcgtcgttc gccatcagga cgaccgcctc gacgtcggcg
     1861 ttgctgtagc cgacgagctt cgccgagaag gtgtcccgga tcgtcgagag gtccacgtcg
     1921 ctcttcaccc tgttcttctt cacgagcgcc ttcgcgaccg cctcgacctc ctcgggggtc
     1981 tgcgagtaga ggaacgggat cttccggtcg agccgcccgg cgcgcttgag gtcgacgtcg
     2041 agcttgtcgg ggcggttcgt catgacgagg aagaggatcc ggccccggtt ctgggtgtcg
     2101 gacatgaact ccttgatccg cgcgatgacg cggctcgagg tccccccgtc cccctcgccg
     2161 tcggtgttcc cgaaggcgcg gtctccctcg tcgatgatga cgacgacctg cccgatcgcc
     2221 ttgatgaccg tcaggatccg ctcgaggttc ccctccgtcg ccccgaccca cttggagcgg
     2281 aagttcttga gcttgatcgt cgggaggccg cactccttcg cgaaggcctc ggcgacgaac
     2341 gtcttccccg tccccatcgg gccggtgaag agcatcccca tcgggacgcg gctccgccgc
     2401 ccctcgcgga tgttgtcggc gaggacgagg aggtctttct tcacctcgtc catcccgccg
     2461 acgacggaga agtcgtggcc cggctcgacg aactcgacga ggccgaagca ctcccgctcg
     2521 aggatctcgc gcttc
//
LOCUS       DAOTEZ010000549         2534 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_33470_length_2534_cov_4.751916, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000549 DAOTEZ010000000
VERSION     DAOTEZ010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33470_length_2534_cov_4.751916"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="P5164_19210"
     CDS             <1..791
                     /locus_tag="P5164_19210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46067.1"
                     /translation="ASDIVKAILFRKLRIVFSPSSPFYTQVKDPRTTTLLVNDLLDEV
                     EDVYEPGLPNLDAFLVDLAAALGDHPVLEQVARTVEHFRVHYVLNSFNGVDEAFDTAV
                     KPFVENLVRTVSERMTLTNLGWVVRSDEIHQGNCRKRPVLLAPPDGRPAARPTRGERE
                     LDALIRETTGLTADPRPRKPVSPVPVRPDPESALARELDALNRMYHRRGNRDDPRDNF
                     DYLTARALHLLDRARPSEFGTARLVGPAEILRSFFPGGAARPSR"
     gene            788..1048
                     /locus_tag="P5164_19215"
     CDS             788..1048
                     /locus_tag="P5164_19215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46068.1"
                     /translation="MKGSARAAAVGVAILAAAAVLASRGRLRELGLRARLAFLAIVTL
                     LGASLLVRGLAADGDRRLYALVAFAVVAPCVVLAWRDLLRRR"
     gene            complement(1053..>2534)
                     /locus_tag="P5164_19220"
     CDS             complement(1053..>2534)
                     /locus_tag="P5164_19220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46069.1"
                     /translation="LAWPLALGAILLAVAGLGVTFRRRPRGAVALLLVLAPAFYSLWF
                     LHSREMSRYSVPFVLVLALPLAAGVEALLRRPPLGLAAMVAGAALAGRASWGEVRYGA
                     TSPTPPTAAIDAVALYGHPGRETIVSDGIFNAFLRLERWERRLRVWPLSDDVLLTKGV
                     RRNRRLVRLADFTTEGESPDRTDPAWRVFRREGRVSRALGNGRLFVVAVRDPAPPLFG
                     PGFGPRESVPGQPSVRWSGPEARLIVPGLQGPPVSLLRGVRYDHEGATTLRARDAETG
                     QIVLERRIEPGAFEVALLAHPVYGPLPKPRELVLSCDRPEAVPAAPGLVRPSRGCFLF
                     REATFSLPPEEVWERLGEERLLDLGRPRDAWADLDGFYDREVHERSGASWRWTSGRAA
                     FTWVPVPGLEPRELVLRARTPGEASVRVEVAFDGRVVGSVEVPSGDFAEVPLSLEPEA
                     RARLGAGDPVRIVLSSPVVVPRDAGLGGDPRRLGIALDRVLVR"
BASE COUNT          349 a          996 c          896 g          293 t
ORIGIN      
        1 ccgcctccga catcgtcaag gcgatcctct tccggaagct ccggatcgtc ttctcgccgt
       61 cgagcccttt ctacacgcag gtgaaggacc cgcgcacgac gacgctcctc gtgaacgacc
      121 tcctcgacga ggtcgaggac gtctacgagc cgggcctccc gaacctcgac gcgttcctcg
      181 tcgacctcgc cgcggcgctc ggcgaccacc ccgtcctcga gcaggtcgcc cggaccgtcg
      241 agcacttccg cgtccactac gtcctgaact ccttcaacgg cgtcgacgag gcgttcgaca
      301 cggccgtgaa gcccttcgtc gagaacctcg tccgcaccgt ttcggaacgg atgacgctca
      361 cgaacctcgg gtgggtcgtg cgctcggacg agatccacca gggcaactgc cggaagcgcc
      421 ccgtcctcct cgcccctccc gacggccgcc ccgcggcgcg cccgacccgc ggcgagcgcg
      481 agctcgacgc cctgatccgg gagacgaccg ggctcacggc cgacccgagg ccgcgcaagc
      541 cggtgtcgcc cgtcccggtg cgccccgacc ccgagagcgc cctcgcgcgg gagctcgacg
      601 cgctcaaccg gatgtaccac cgccgcggga accgcgacga cccgcgggac aacttcgact
      661 acctcacggc gcgcgccctg cacctcctcg accgggcccg cccgtcggag ttcgggacgg
      721 ctcgcctcgt cggccccgcc gagatcctcc gttccttctt tcccggcggg gcggcgcgcc
      781 cgtcccggtg aagggctcgg cccgggccgc ggccgtcggc gtcgcgatcc tcgccgccgc
      841 ggccgtcctc gcctcgcgcg ggcgcctgcg ggagctcggc ctccgggccc ggctcgcgtt
      901 cctcgcgatc gtcaccctcc tcggagcctc gctcctggta cgcgggctgg ccgcggacgg
      961 ggaccgccgg ctctacgccc tcgtggcgtt cgccgtcgtc gcgccgtgcg tcgtcctcgc
     1021 ctggcgggac ctcctccgcc gccggtagac ggctatcgga ccaggacgcg gtcgagggcg
     1081 atcccgaggc gccgcgggtc ccctccgagg ccggcgtccc gcgggacgac gaccggcgag
     1141 gagagaacga tcctcaccgg atctccggcg cccaggcgcg cgcgcgcctc cggctcgagg
     1201 gagaggggga cctcggcgaa gtcgcccgac ggcacctcga cgctccccac gacgcgcccg
     1261 tcgaacgcga cttcgacgcg gacgctcgcc tcgccgggcg tccgtgcccg gaggacgagc
     1321 tcgcgcggct cgagacccgg cacggggacc cacgtgaacg cggctcggcc ggaggtccag
     1381 cgccagctcg cgccgctcct ctcgtggacc tcccggtcgt agaacccgtc gagatcggcc
     1441 caggcgtcgc gcgggcgacc gaggtcgagg aggcgctcct cgccgagccg ctcccagacc
     1501 tcctcggggg ggagggagaa cgtcgcctcc cggaacagga agcagcctcg cgagggccgc
     1561 acgaggccgg gcgcggccgg gactgcctcc ggccgatcgc acgacaggac gagctcgcgc
     1621 ggcttcggga gaggcccgta gaccggatgc gcgaggaggg cgacctcgaa cgcgcccggc
     1681 tcgatccgcc gctcgagaac gatctgtccc gtctcggcgt cccgcgccct gagcgtcgtc
     1741 gccccttcgt gatcgtagcg gacgcctcgc aggagcgaga ccggcgggcc ctggagcccc
     1801 gggacgatca gccgcgcctc cggtccggac cagcgcaccg agggctgccc cgggacgctc
     1861 tccctcggcc cgaatccggg cccgaagagc ggcggcgcgg gatcgcgcac ggcgaccacg
     1921 aagagccggc cgttcccgag cgcccgagag accctccctt cccgccggaa gacccgccag
     1981 gcggggtcgg tccggtccgg ggactccccc tccgtcgtga agtcggcgag gcggacgagg
     2041 cggcggttcc gccgcacgcc cttcgtcagc aggacgtcgt ccgagagcgg ccagacacgg
     2101 aggcgcctct cccagcgctc cagccgaagg aacgcgttga agatcccgtc ggagacgatg
     2161 gtctcccggc ccgggtggcc gtagagggcg acggcgtcga tcgccgccgt gggcggagtc
     2221 gggctcgtcg ccccgtaccg gacctcgccc cacgaggcgc gaccggcgag ggccgccccg
     2281 gccaccatcg ccgcgagccc gagcggaggc ctccggagca gagcttccac tcccgcggcc
     2341 aggggaagcg ctaggacgag gacgaacggg accgagtagc gcgacatttc ccggctgtgg
     2401 aggaaccaga gcgagtagaa cgccggggcg aggacgagga ggagcgcgac ggcacctcgc
     2461 gggcgccggc ggaacgtgac cccgaggccg gcgacggcga gcaggatggc tcccagcgcg
     2521 agcggccacg cgag
//
LOCUS       DAOTEZ010000550         2524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_33676_length_2524_cov_5.639530, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000550 DAOTEZ010000000
VERSION     DAOTEZ010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33676_length_2524_cov_5.639530"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="P5164_19225"
     CDS             <1..174
                     /locus_tag="P5164_19225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013851004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRY46070.1"
                     /translation="GDLGEGGVEAGGAHAAGLNFGDCFAYALARSLGEPLLFKGDDFA
                     KTDLATAGARPGA"
     gene            427..>2524
                     /locus_tag="P5164_19230"
     CDS             427..>2524
                     /locus_tag="P5164_19230"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HRY46071.1"
                     /translation="MRCRRSVVSWIVLGLAVQLAALPAAAASVAAAPLGPLVDAPGGR
                     GEMELVRFDDALAPMLLAADPEQEVEIRDWPVTPGRRATVRLRRFDVYAPDARIVVIE
                     DGMSRDEPRSRLAFFRGAVADGLEAGSRLVTWLDPETGRVGGFVAATSGTVLLEPARR
                     DDREVTRVLMSDTLPESGPGLAWSCGVKEEGGDFSWREEMRRAREAAAAAHPSSVAAL
                     GTLTKTAVIAFDTDAEYLDLRFDNSASAATSHIAQLVAGMSLIYERDVASVSGDGVRI
                     LQGYTILRIGEASDPYTDTVGSADASKLIEFRDYWNANYPTTVVRRALAALLSGKQGS
                     EFSASGIAAVGALCSTSGYSVDQLFTYPVSVTTDLGILAHEVGHNFGAYHTHSCYYGT
                     PPIDTCVTPEGSCTPATGCPGSATFNGVTTNGTLMSYCHVVGCNPRNLVFHTRSVTDN
                     PADPYFVDSLLQEVANAGCLASLSGGVVLPAPTVSSVSPASGPLTGGSSVTITGTNFV
                     NGATVAFVELPTNDVFGTPSAKAASTTFVNSTQLTVTTPSAALAGAVDVVVMNPDRQT
                     VKRASGFTYASGPPAPAVTSVSPAVGTTSGGTAVTITGSSFVAGATVRFDGVLATGVG
                     FVSSTTLTATTPAHAAGAVNVTVTNPDLQVGTLVNGFSYGAPLISTFTANPVLIASGG
                     ASTLTWATSGATSVTLN"
BASE COUNT          357 a          922 c          858 g          387 t
ORIGIN      
        1 ggcgacctgg gcgaaggggg cgtcgaagcc gggggcgcgc acgcggccgg cctcaacttc
       61 ggagactgct tcgcctacgc gctcgcgcgc tctctcggcg agcccctgct cttcaagggg
      121 gatgacttcg cgaagacgga cctcgcgaca gcaggggccc gccccggagc gtgagctcga
      181 tggcgttgaa cggttcgacg cggagggcct cgtcgccgcc gcgacggcga cgtcggcgct
      241 gggcacgccg cctcctggcg gcctgccgtg gggtggcttc acccccatag ggcgtcgcct
      301 gttcgcgagg cgccgcgaag gccgcgaggt ccttccggcg tgactggatc ggcgctcgca
      361 gaaggactag attggccctg gggcggggct ctccggagca gcccacctgc ccctcggagg
      421 cccggaatgc ggtgtcgcag atcggtcgtt tcgtggatcg tcctcggcct cgccgtccag
      481 ctggcggcgc tgccggccgc ggcggcgtcc gtggccgcgg ctccgctcgg accgctcgtc
      541 gatgcgccgg gcggccgtgg cgagatggag ctggttcgct tcgacgacgc cctcgcgccc
      601 atgctgctcg cggcggatcc ggagcaggag gtcgagatcc gggactggcc ggtgacaccc
      661 ggacggcgag cgaccgtgcg ccttcgccgg ttcgacgtct acgcgcccga cgcgaggatc
      721 gtcgtcatcg aggacggcat gtctcgcgac gagccccgct cgaggctggc gttcttccgg
      781 ggcgccgtcg ccgacggtct ggaggcgggg agccgcctcg tcacgtggct cgacccggag
      841 accgggcgcg tcggaggttt cgtcgccgcc acgagcggca ccgtcctcct cgagccggcc
      901 cgccgcgacg atcgcgaggt cacccgggtc ctgatgtccg acacgctgcc ggaatccggg
      961 ccgggcctcg cctggagctg cggcgtgaag gaggaaggag gggacttctc gtggcgggag
     1021 gagatgagac gggcgcgcga ggcggcagca gccgcccacc cgtcgtccgt cgcggccctc
     1081 gggaccctca cgaagaccgc cgtcatcgcc ttcgacaccg atgccgagta tctcgatctg
     1141 cgattcgaca acagcgcgag cgccgcaacg agccacatcg cccagctcgt cgccgggatg
     1201 tcgctgatct acgagcggga cgtggcctcc gtctctggag acggggtccg gatccttcag
     1261 ggatacacga tccttcgcat cggcgaggcg agcgacccgt acacggacac cgtcggctcg
     1321 gccgacgcct cgaagctgat cgagttccgg gactactgga acgcgaatta ccccaccacc
     1381 gtcgtcaggc gggcgcttgc cgccctcctc tccggaaagc aggggagcga gttctcggcg
     1441 tcgggtattg ccgccgtcgg cgctctctgc tccacgtccg gctacagcgt cgaccagctc
     1501 ttcacttatc ccgtctccgt cacgacggac ctcggcatcc tcgcgcacga ggtcgggcac
     1561 aatttcggcg cctaccacac gcacagctgc tattacggca ccccccccat cgacacctgc
     1621 gtgaccccag aggggagctg tacgcccgcc accgggtgcc ccggctccgc cactttcaac
     1681 ggcgtgacga cgaacggcac cctcatgagc tactgccacg tcgtcggctg caacccacgg
     1741 aacctggtgt tccacacccg gtcggtcacc gacaacccgg ccgatccgta tttcgtcgac
     1801 tcgctgctcc aggaggtggc caacgccggc tgcctcgcct ccctcagcgg cggcgtcgtc
     1861 ctcccggcgc ctacggtctc gagcgtctcg ccggcttcgg gtcccctgac gggaggctcg
     1921 agcgtcacca ttaccggaac gaacttcgtg aacggggcta ccgtcgcctt cgtcgagctt
     1981 ccgacgaacg acgtcttcgg gactccgagc gcgaaggcgg cctccaccac tttcgtcaac
     2041 tcgacgcagc tgacggtgac gacgccctcg gcggctctcg ccggcgcggt ggacgtcgtc
     2101 gtcatgaacc cggaccggca gacggtgaag cgcgcgagcg gcttcaccta cgcgtcgggc
     2161 ccgccggccc ctgccgtcac ctccgtgagt cccgcggtcg gcactacctc cggcggaacg
     2221 gccgtcacga tcacgggctc gagcttcgtc gccggggcga ccgtccgatt cgacggcgtg
     2281 ctggccaccg gtgtcgggtt cgtttcgtcc accacgctca cggcgacgac cccggcgcac
     2341 gcggccggcg ccgtcaacgt caccgtcacg aaccccgacc ttcaagtcgg cacgctcgtg
     2401 aacggcttca gctacggtgc gcctctcatc tccaccttca cggcgaaccc ggtgctgatc
     2461 gcgagcggag gagcgtcgac gctgacgtgg gcgacgagcg gagcgacgag cgtgacgctg
     2521 aacg
//
LOCUS       DAOTEZ010000551         2517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_33826_length_2517_cov_3.688871, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000551 DAOTEZ010000000
VERSION     DAOTEZ010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33826_length_2517_cov_3.688871"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="P5164_19235"
     CDS             <1..186
                     /locus_tag="P5164_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46072.1"
                     /translation="VGAGSDVMKRIAAPMVGGLATSFLLELLVYPAIYMLWKQRSLPK
                     PGPDHSEPSLEGAALPA"
     gene            226..705
                     /locus_tag="P5164_19240"
     CDS             226..705
                     /locus_tag="P5164_19240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46073.1"
                     /translation="MNHTSPLPRWTAALAAAVLSASALAGPAAPPKAASKTPVFKDVK
                     AQTLEFMRYTDMSLAPEQEKIKDDVLSAIPTVCCKKFSMKTCCCPCNMAMTIWGLSNY
                     MLVVKGANAAELKSAVLDWVKFIGPAGYTGDACFKGGCSRPFSKNGCGGMDHKNVVF"
     gene            complement(1008..1952)
                     /locus_tag="P5164_19245"
     CDS             complement(1008..1952)
                     /locus_tag="P5164_19245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46074.1"
                     /translation="MRSLRLRLRAWVVVTTTVAVGAVTAAGVLDERRQIRRAESAYIG
                     AFLEHLAAMPELRSDSSTVQAHLGALSGSLARAGGKVALVALKPSDPVPLGAGAVACQ
                     RLSLSDGEWALHYWSDGRFVRAQIRRALLTRLLLAVGLVLAIVAGLEWMLRRNLLRPL
                     STISRQIERITEGGGWLAHVPETDEELQRLTRALRALGPGLEAQTRQWIETERRVTAA
                     SVLARIREATLEPLRSAQLELSQAEAAFTTDAATKTRLRRAARSVSRLTETLRGAEAT
                     WFRDRGGARAASDEGTGKQADDGAVIHRSPTSTGVAAG"
     gene            complement(1949..>2517)
                     /locus_tag="P5164_19250"
     CDS             complement(1949..>2517)
                     /locus_tag="P5164_19250"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRY46075.1"
                     /translation="RTLSLLRPEPGERVLDLGCGTGSLTVRLKQEFPRVEVIGCDVDP
                     GALGLARRKAAVAGVSIRWCLGPAHDPPLPDRSVDLVTSTLLFHHLRPDGKRAALAAA
                     LRLLEPGGRLVVVDFTRPSDFLRRIAFSGVRLLDGLEVTADHAAGRFAEIVESAGFER
                     VTEADRLDTPVGTIAFLTAARRREGRGG"
BASE COUNT          385 a          903 c          891 g          338 t
ORIGIN      
        1 gtcggggccg gctccgacgt gatgaagcgg atcgccgcac cgatggtggg cggcctcgcg
       61 acgtcgtttc tcctggaact cctcgtctat cccgccatct acatgctctg gaagcagcga
      121 agcctcccga aacccgggcc cgatcactcg gagccgagcc tcgagggcgc ggcacttccc
      181 gcctgacacc ggtcgaaccg aactgacgga agcaggagtc acgacatgaa ccacacctcc
      241 cccctccctc gctggaccgc cgctctcgcg gccgccgtcc tttccgcttc ggccctggcc
      301 ggcccggccg ccccgccgaa ggccgcctcg aagacgcccg tcttcaagga cgtcaaggcc
      361 cagacgctcg agttcatgcg gtacaccgac atgagcctcg cgccggagca ggagaagatc
      421 aaagacgacg tcctctccgc catcccgacg gtctgctgca agaagttctc gatgaagacc
      481 tgctgctgcc cgtgcaacat ggcgatgacg atctgggggc tctcgaacta catgctcgtc
      541 gtgaaggggg cgaacgccgc cgagctgaag tcggcggtcc tcgactgggt gaagttcatc
      601 gggccggccg gctacacggg agacgcctgc ttcaagggcg gctgcagccg cccgttctcg
      661 aagaacggct gcggcggcat ggaccacaag aacgtcgtct tctgaggagg cgaacgacga
      721 ttggtgtccc gacgagcctc cctaccttgc tcctggcgag cggctcggcg ccggcacggg
      781 agcacgtcca cggcggtccg ggtcccgccc cgcaggcggg gccccggccc acctctgctc
      841 ggagtgtccg ggaggtgctg cacgaggagg ctcgcgccga gctcggggca gcaaggacgc
      901 tcgagggatt ttggcttcac ctgccgtctg cggtcgccac gctgacgcgg aagctcgcgc
      961 tcgctgaatc gcgcctcgcc gagcggcgag ggcagggagc cgcgagctca gccggcggcc
     1021 acgcccgtcg aagttggcga gcggtggatg acggccccgt cgtcggcctg cttccccgtc
     1081 ccctcgtccg acgcagcgcg ggcgcccccg cgatcgcgaa accacgtcgc ctccgcgccg
     1141 cggagcgttt ccgtgagccg gctgacggag cgggcggcgc gcctgagccg cgtcttcgtc
     1201 gcggcgtcag tggtgaaagc cgcctcggcc tggctcagct cgagctgcgc ggaccgaagc
     1261 ggctcgagcg tggcttcgcg gatcctcgcg aggaccgatg ccgcggtgac gcgccgctcg
     1321 gtctcgatcc actgacgggt ctgcgcctcg agcccggggc cgagggcgcg gagcgctcgc
     1381 gtcagccgct ggagctcctc gtccgtctca gggacgtgcg cgagccagcc cccgccctcc
     1441 gtgatccgct cgatctggcg cgagatcgtc gagagcggtc ggaggaggtt tcgacggagc
     1501 atccattcga gccctgcgac gatggcgagg acgaggccga cggcaagcag gagccgcgtg
     1561 agcaacgccc gccggatctg cgcccggacg aatcggccgt cgctccagta gtgcagcgcc
     1621 cactcgccgt ccgagaggga aagacgctgg caagcgacgg cgccggcgcc gagcgggacc
     1681 ggatcggagg gcttcaaggc tacgagcgcg acctttcccc cggccctcgc gagggagccc
     1741 gacagggcac cgaggtgcgc ttgcaccgtg gaagagtcgc tccgaagctc cggcatcgca
     1801 gccaggtgct cgaggaacgc accgatgtag gccgactcgg ctcgccggat ctggcgccgc
     1861 tcatcgagga cgccggccgc cgtcaccgct ccgacggcga cggtcgtcgt caccacgacc
     1921 caggcgcgga ggcggaggcg gaggctcctc atccgccgcg accctcgcgg cggcgggccg
     1981 ccgtgaggaa ggcgatcgtg ccgacgggcg tgtcgaggcg atcggcctcc gtcacgcgtt
     2041 cgaacccggc ggactccacg atctccgcga accgccccgc cgcgtggtcc gccgtgacct
     2101 cgagaccgtc gaggagccgg actccggaga aggcgatccg acgaaggaag tccgatggcc
     2161 tcgtgaagtc gacgacgacg agccgtccgc ccggctcgag cagcctcagg gcggcggcca
     2221 gcgcagcgcg cttgccgtcc ggccggaggt ggtggaagag cagcgtcgag gtgacgagat
     2281 ccaccgatcg gtccgggagc ggcgggtcgt gggcggggcc gaggcaccac cgtatcgaga
     2341 ccccggccac ggcagccttc cgccgggcca ggccgagcgc tcccgggtcc acgtcgcaac
     2401 cgatcacttc gaccctcggg aactcctgct tgagccggac cgtgagggag cccgtgccgc
     2461 agcccaggtc gaggaccctc tctccgggct ccggccggag gaggctcagg gtgcggc
//
LOCUS       DAOTEZ010000552         2504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_34107_length_2504_cov_6.859943, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000552 DAOTEZ010000000
VERSION     DAOTEZ010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34107_length_2504_cov_6.859943"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1749
                     /locus_tag="P5164_19255"
     CDS             <1..1749
                     /locus_tag="P5164_19255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HRY46076.1"
                     /translation="ELDPADVARMTKAFIDEAGWSEVGLLSLSSADYSQIEPLVACLA
                     PELAKTKVSISLPSLRAEAFSVALADAVSEVRKSGFTFAPETGSDRLRRVINKTFTND
                     DMVKAAGVAFERGWDLIKVYTMIGLPSETEADLDELVTLVADILAEGRKKGVRATVHV
                     SVGSFVPKSFTPFQWSAFDGVEQLLQKLAYLKEKLRPVRGAKLKWHEPKEAEIEALLS
                     LGDRRMSSALLEVWRRGGRFDGWSEHFSWPRWTEALEAAGIPKERHLRQKDLKETLPW
                     DVVDASIRKPFLVVEWKKALQEMATEDCKWGRCTACGVPGNGEDTVLANGMPAAFSFA
                     LPPLVAAAPAAPGPAEAGSGASLPSAAGVASGVADARDEGRGSAYADKAKGAAYRMKA
                     TPDLLPLRERRTSRGGASASAAVAKSWRVSFEKLGDARYLSHRNVMDVLERALRAARV
                     PVRYTEGFNPHIRLSMGPALPLGSESRHELFDLDVVEPLTPEHLAAVNDRLPPGMRLL
                     GWTELPPGAPSLGKAATEGVYRFTLPNGEVIVERLRLSGEGATTPKRHIETTWGLAPE
                     DQHAIRVVREETVLTR"
     gene            1773..2147
                     /locus_tag="P5164_19260"
     CDS             1773..2147
                     /locus_tag="P5164_19260"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRY46077.1"
                     /translation="MPFTIRCHATFEAAHHLRDYVGGPEPVHGHSWKVEVALGTDALG
                     DYDLSVDFVPTERLVKELAARLHDRDLNGVPPFDVRNPTAENVALWFADEVRRAGVLS
                     GGTRLEEVTVWEGPRNSVTWRP"
     gene            complement(2197..>2504)
                     /locus_tag="P5164_19265"
     CDS             complement(2197..>2504)
                     /locus_tag="P5164_19265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46078.1"
                     /translation="NYDFRRTEMCIIPYGTQLGEISFCAYNTGAGWRQIIEEMHQTAT
                     LADWYRDKGRHPIYAGGKAVPLPTYRPRPAVVPEELPEAARAAVGMGLPSLGTASGR"
BASE COUNT          357 a          920 c          883 g          344 t
ORIGIN      
        1 gagctcgacc cggccgacgt cgcgcggatg acgaaggcgt tcatcgacga ggcgggctgg
       61 tcggaggtcg ggctgctttc gctctcctcc gccgactact cgcagatcga gccgctcgtc
      121 gcctgcctcg cccccgagct cgcgaagacg aaggtctcca tctcgctccc ttcgcttcgc
      181 gccgaggcct tctccgtcgc gctcgccgac gccgtctccg aggtgcggaa gtcgggcttc
      241 acgttcgccc ccgagaccgg ctcggatcgc ctccgccgcg tgatcaacaa gacgttcacg
      301 aacgacgaca tggtcaaggc cgcgggcgtc gcgttcgagc gcggctggga cctcatcaag
      361 gtctacacga tgatcggcct cccctccgag acggaggccg acctcgacga gctcgtcacc
      421 ctcgtcgcgg acatcctcgc cgagggacgg aagaagggcg tgagggccac ggtccacgtc
      481 tccgtcggct ccttcgtccc gaagagcttc acgccgttcc agtggtccgc gttcgacggc
      541 gtggagcagc tcctgcagaa gctcgcgtac ctgaaggaga agctccggcc cgtccgcggg
      601 gcgaagctga agtggcacga gccgaaggaa gccgagatcg aggcgctcct ctccctcggc
      661 gaccggcgga tgtcgagcgc gctcctcgag gtctggagga ggggcgggag gttcgacggc
      721 tggtcggagc acttctcctg gccgcgctgg accgaggcgc tcgaggccgc cggcatcccg
      781 aaggagcggc acctgcggca gaaggacctg aaggagacgc tcccctggga cgtcgtcgac
      841 gcctcgatcc gcaagccctt cctcgtcgtg gagtggaaga aggccctgca ggagatggcg
      901 acggaagact gcaagtgggg ccgctgcacg gcctgcggcg tccccggcaa cggcgaggac
      961 accgtcctgg cgaacgggat gccggccgcg ttctcgttcg cgctcccccc gctcgtcgcc
     1021 gcggcgcccg ccgccccagg acctgcggag gccggttccg gcgcttccct cccgtccgcc
     1081 gccggcgtcg cgagcggcgt cgccgacgcg cgcgacgagg gacgcggctc ggcctacgcc
     1141 gacaaggcga agggggctgc gtaccggatg aaggcgacgc ccgacctgct cccgctccgc
     1201 gagcggcgaa cctcgcgggg cggcgcgagc gcgagcgcgg cggtcgcgaa gagctggcgg
     1261 gtctcgttcg agaagctcgg cgacgcccgc tacctctcgc accggaacgt catggacgtc
     1321 ctcgaaagag cgctccgcgc cgcgcgcgtc cccgtccgct ataccgaggg cttcaacccg
     1381 cacatccgcc tctcgatggg gccggcgctc ccgctcggct ccgagtctcg ccacgagctc
     1441 ttcgacctcg acgtcgtcga gccgctgacg cccgagcacc tcgccgccgt caacgaccgc
     1501 ctcccgcccg gcatgcggct cctcggctgg acggagctgc cgcccggcgc accgtccctc
     1561 gggaaggcgg cgacggaagg ggtctaccgc ttcacgctgc cgaacggcga ggtgatcgtc
     1621 gagcggctgc gcctctcggg cgagggggcg acgacgccga agcggcacat cgagacgacg
     1681 tggggcctcg cccctgagga ccagcacgcc atccgcgtcg tccgcgagga gacggtcctg
     1741 acgcgctgag cgcgcgacgg agagggagcg ccatgccgtt cacgatccgc tgccacgcga
     1801 cgttcgaggc cgcccaccac ctgcgcgact acgtcggagg tccggagccg gtccacgggc
     1861 actcctggaa ggtcgaggtc gccctcggga ccgacgcgct cggcgactac gacctctccg
     1921 tcgacttcgt cccgacggag cggctcgtga aggagctggc cgcgcgcctc cacgaccgcg
     1981 acctcaacgg cgtcccgccg ttcgacgtga ggaacccgac cgccgagaac gtcgcgctct
     2041 ggttcgcgga cgaggtgcgg agagcgggcg tcctgtcggg gggcacgcgc ctggaggagg
     2101 tcacggtctg ggagggcccg cgaaactccg tcacctggag gccctgacca ccccgggagg
     2161 cgggccgcgc tccggcccgc ccccctccgc cggcccctac ctgcccgagg cggttcccag
     2221 ggacggcagc cccatcccga cggccgccct cgcggcctcg gggagctcct cgggcacgac
     2281 cgcgggacgg gggcggtacg tcgggagcgg gaccgccttg ccgcccgcgt agatcgggtg
     2341 gcgccccttg tcgcggtacc agtcggcgag cgtggccgtc tggtgcatct cctcgatgat
     2401 ctgcctccag cccgcgccgg tgttgtaggc gcagaaggag atctcgccaa gctgcgtgcc
     2461 gtacgggatg atgcacatct ccgtccggcg gaagtcgtag ttga
//
LOCUS       DAOTEZ010000553         2500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_34199_length_2500_cov_4.118609, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000553 DAOTEZ010000000
VERSION     DAOTEZ010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34199_length_2500_cov_4.118609"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="P5164_19270"
     CDS             <1..976
                     /locus_tag="P5164_19270"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY46079.1"
                     /translation="ENRLLQVAANVKGRIASIPVDLGSSVAKGQPLLVIESVDLGRAR
                     EEFVRELSAFSVSARAFERARKLVEEKAISAGEFQSREGDYLARKASLASAERTLHLY
                     GDDDEALRRLRAAAASDAHTAEDGAALTLRAPFAGRVVERKVTPGALFEALQPLLTLA
                     DLSTVWVFLDVYEKDLSQLHEGLAVTLRTEAYPQETFPGKIDFLGGVIDAQTRSTRVR
                     ATVMNRSGRLRPGLFVKALVDVPRPKSESHPVVAVPQGAIQSLEGRPTVFVQKEPGVF
                     VRHAVETGHTFEGFTEVLSGVKSGDVVVTEGSFVLKSEFAKASLAEEH"
     gene            978..>2500
                     /locus_tag="P5164_19275"
     CDS             978..>2500
                     /locus_tag="P5164_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRY46080.1"
                     /translation="MLRKIVDLSLENRALVLLASLLLVVGGGYALTQLPVDAVPDITN
                     VQVQVLTKAPALGPVEMEQFVTYPVEAAMNGLPRLKEIRSVSRYGLSAVTIVFEDGVN
                     IFFARQLVAERLVQAREAIPEGLGNPEMGPVTTGLGDVFQFTVEGEGVSAMERRSILD
                     WMIAPRLRAVPGVTEVNTWGGLPKQYQVLVDPARLRAHGLSLREVFEAVERGNANAGG
                     GYIEHNREQYIVRGEGLVGSISDIEKIVLKADEHGTPVTVGSIAQVREGAMMRIGVAT
                     KDGRGETVIGLVQMLAGENALDVATRARKAVEELQPTLPKGVTIVPFYDRASFVRRVI
                     RTVETNLLEGSILVVAVLFLFLGNVRAGLIVASAIPLSMLLAFTGMVETRISANLMSL
                     GAIDFGLIVDGAVVLVENVVRRLSEPEGREKTVRQLTAEAAHEVVRPITFGIGIIALV
                     YLPILSLEGVEGKMFKPMAWTVVFALAGSLLLTLTLTPVLASLFLKKTGHDHEPRFVG
                     "
BASE COUNT          374 a          902 c          830 g          394 t
ORIGIN      
        1 cgagaaccgc ctcctccagg tcgccgcgaa cgtgaagggg cgcatcgcgt cgatcccggt
       61 cgacctcggc tcgagtgtcg cgaagggtca gcccctcctc gtcatcgaga gcgtcgacct
      121 cggccgggcg cgcgaggagt tcgtgcggga gctctccgca ttcagcgtct cggcgcgcgc
      181 tttcgagcgg gcccgcaagc tcgtggagga gaaggcaatc agcgccgggg agttccagtc
      241 ccgcgaaggg gattacctcg cccggaaggc gagtctcgcc tccgccgaaa ggaccctcca
      301 cctctacggc gacgacgacg aggcgctccg ccgcctccgc gctgccgccg cttcggacgc
      361 gcacaccgcc gaggacggcg ccgccctcac gcttcgcgcg ccgttcgccg ggcgcgtcgt
      421 cgagcgcaag gtcacccccg gcgcgctctt cgaggcgctt cagccgctcc tgacgctcgc
      481 cgacctctcc acggtctggg tcttcctcga cgtctacgag aaggacctct cgcagctcca
      541 cgaggggctc gcggtcacgc ttcggaccga ggcgtacccg caggagacct tccctgggaa
      601 gatcgacttc ctcggcgggg tcatcgacgc ccagacgcgg agcacccgcg tccgtgcgac
      661 cgtcatgaac cgctccggaa ggctgcggcc cggcctcttc gtcaaggccc tggtcgacgt
      721 cccccggccg aagagcgagt cccatcccgt cgtcgcggtc ccgcaggggg cgatccagtc
      781 gctcgagggc cggccgacgg tcttcgtaca gaaggagccc ggtgtcttcg tccggcacgc
      841 cgtcgagacg ggccacacgt tcgagggttt cacggaggtc ctctccggcg tgaagtcggg
      901 agacgtcgtc gtgacggaag ggagcttcgt cctgaagtcg gagttcgcga aggcctcgct
      961 cgccgaggag cactgagatg ctccggaaga tcgtcgacct cagcctcgag aaccgcgccc
     1021 tcgtcctcct cgcctcgctc ctcctcgtcg tcggcggagg gtacgccctt acacagctcc
     1081 cggtcgacgc cgtccccgac atcaccaacg tccaggtcca ggtcctgacg aaggccccgg
     1141 ccctcggccc ggtcgagatg gagcagttcg tcacctaccc ggtcgaggcg gccatgaacg
     1201 gcctcccgcg cctgaaggag atccgctcgg tctcacgcta tggcctctcc gccgtgacga
     1261 tcgtcttcga ggacggggtg aacatcttct tcgcccgcca gctcgtcgcc gaacgcctcg
     1321 tacaggcgcg cgaagcgatt cccgaagggc tcgggaaccc ggagatgggg ccggtgacga
     1381 ccggcctcgg cgacgtcttc cagttcacgg tcgaggggga gggggtctcg gccatggagc
     1441 gccggtcgat cctcgactgg atgatcgcgc ctcgcctgcg tgccgtcccc ggcgtcaccg
     1501 aggtgaacac ctggggcggc ctcccgaagc agtaccaggt cctcgtcgac ccggcccggc
     1561 tccgggcgca cggcctttcg ctccgcgagg tcttcgaggc ggtggagcgc gggaacgcga
     1621 acgccggagg cgggtacatc gagcacaacc gcgagcagta catcgtgcgg ggcgaggggc
     1681 tcgtcggctc gatctccgac atcgagaaga tcgtcctgaa ggccgacgag cacgggaccc
     1741 ccgtgaccgt cgggagcatc gcgcaggtgc gcgaaggcgc gatgatgcgg atcggcgtcg
     1801 cgacgaagga cggtcgcggc gagacggtca tcggcctcgt ccagatgctg gcgggcgaga
     1861 acgccctgga tgtcgccacc cgggcgcgca aggcggtcga ggagctccag ccgacgctgc
     1921 cgaagggcgt gacgatcgtc cccttctacg accgggcgtc gttcgtgcgc cgcgtcatcc
     1981 gcacggtcga gacgaacctc ctcgagggga gcatcctcgt cgtcgccgtc ctcttcctct
     2041 tcctcggcaa cgtccgcgcg ggactgatcg tcgcctcggc gatcccgctc tcgatgctcc
     2101 tcgccttcac gggaatggtc gagacgcgca tctccgcgaa cctcatgagc ctcggcgcca
     2161 tcgacttcgg gctgatcgtc gacggcgcgg tcgtcctcgt cgagaacgtc gtccgccgcc
     2221 tatcggagcc ggaggggcga gaaaagacgg tccgacagct gacggcggaa gccgcccacg
     2281 aggtcgtccg gccgatcacc ttcggcatcg gcatcatcgc cctcgtctac ctccccatcc
     2341 tcagtctcga gggtgtcgag gggaagatgt tcaagcccat ggcctggacg gtcgtcttcg
     2401 ccctggccgg ctccctcctc ctcacgctca cgctcacgcc ggtcctcgcg tcgctcttcc
     2461 tgaagaagac ggggcacgac cacgagcctc gcttcgtcgg
//
LOCUS       DAOTEZ010000554         2498 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_34249_length_2498_cov_4.604585, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000554 DAOTEZ010000000
VERSION     DAOTEZ010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34249_length_2498_cov_4.604585"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="P5164_19280"
     CDS             complement(<1..1202)
                     /locus_tag="P5164_19280"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRY46081.1"
                     /translation="MAEDDAVRIGSNLERMAKLGRLVASSAEPLARRLTDSARIPAAV
                     ATSDVLGLARLLSEYGTLLSRLAAGTIDHPLWAGRTFDPAARAPRGAVAATLPEDGAA
                     TAFGLLFAPTFLCGADLAVHPAPRTRSLFQTLEALAAEEGLGSLLKRRDGASLFSRAD
                     RPAFLAEHLDPTCRTAVLALHVGSGRVEEATRQSVLRKKEEFRLLVEGPAMDRFVVAA
                     PLADPAPAARALVDAALAGGGRARGRAEVVDVHESVLPQLLPLVVELAATAVVGDPYE
                     APTRVGPFPLERARAVAARVDDALARGATLAYATERAGKLPLLGGGGDRPSFLLPPYR
                     GSEPFAFLPFVVLAGLDDGALLASEPAGGPVLAIRAFREDRELVPRILGSPCGHGATL
                     FGDDAEADA"
     gene            1305..2075
                     /locus_tag="P5164_19285"
     CDS             1305..2075
                     /locus_tag="P5164_19285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011749182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY46082.1"
                     /translation="MELNGAVVIVSGGASGLGEATARRLVARGARAGILDLPSSRGEE
                     VAASLGGAALFVPADVTDGAGVEGAVGRVVSAFGRLDVAVTCAGVGTSGRVLDKTGAP
                     HALDLFERIVRVNLVGTFNVLRIAASAMYRNAPNADGERGVVVATASVAAYDGQVGQV
                     AYAASKGGVVSMTLPVARDLAQWGIRCVTIAPGIFDTPMLATVKEEFRARLAAGVPFP
                     SRLGDPAEYARLVEQIVGSPYLNGEVVRLDGALRMAPR"
     assembly_gap    2166..2265
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          317 a          862 c          934 g          285 t
ORIGIN      
        1 gcgtcggcct cggcgtcgtc gccgaagagc gtggctccgt ggccgcaggg cgacccgagg
       61 atccggggaa ccagctcgcg gtcctcgcgg aaggcccgga tcgcgaggac gggcccgccc
      121 gcgggctcgg aggcgaggag cgccccgtcg tcgaggccgg ccaggacgac gaacgggagg
      181 aacgcgaacg gctcgctgcc gcgataggga ggaaggagga acgacggccg gtcccctccg
      241 ccgccgagga gcggcagctt ccccgccctc tccgtggcgt acgcgagcgt ggcgccgcgc
      301 gcgagagcgt cgtccacccg ggcggcgacg gcgcgggccc tctcgagcgg gaagggcccg
      361 acccgcgtcg gcgcctcgta cgggtcgccg acgaccgcgg tcgcggcgag ctcgacgacg
      421 agcgggagga gctgcgggag gaccgactcg tggacgtcga cgacctcggc ccgcccccgg
      481 gcgcgcccgc ccccggcgag cgccgcgtcg acgagggccc gggccgccgg cgccgggtcg
      541 gcgagcgggg cggcgacgac gaagcggtcc atggccggcc cttcgacgag gagccggaac
      601 tcctccttct tccggaggac gctctggcgc gtcgcctcct cgacccggcc cgatccgacg
      661 tggagcgcca ggaccgccgt ccggcaggtc gggtcgaggt gctcggcgag gaacgcgggc
      721 cgatccgcgc ggctgaagag gctcgcgccg tcgcgccgct tcaggaggct cccgaggccc
      781 tcctcggccg cgagcgcctc gagcgtctgg aagaggcttc gggtccgcgg cgccgggtgg
      841 acggcgaggt ccgcgccgca gaggaacgtc ggagcgaaga ggagcccgaa ggccgtcgcc
      901 gctccgtcct caggaagggt ggccgcgacg gcgccgcgcg gcgcgcgcgc ggccggatcg
      961 aaggtccgac ccgcccagag cggatggtcg atcgtcccgg cggcgaggcg cgagaggagc
     1021 gtcccgtact ccgagaggag gcgggcgagg ccgaggacgt cgctggtcgc gaccgcggcc
     1081 gggatccgcg ccgagtccgt gaggcggcgg gcgagcggct cggcgctcga ggcgacgaga
     1141 cgccccagct tcgccatccg ttcgaggttc gacccgattc gtacggcatc gtcttcggcc
     1201 atgtccgtct ccctccctcg cgcaggacca ccgggcatct tacggctcgc cggagcgggc
     1261 gggacccttc gaccctgcgg tagccttcgc ggggaggacg tgccatggag ctgaacggag
     1321 cggtcgtcat cgtcagcggg ggcgcctcgg ggctcggcga ggccacggcg aggcgcctcg
     1381 tcgcgcgcgg cgcgcgggcc gggatcctcg acctcccgtc ctcgcgcggc gaggaggtcg
     1441 ccgcctccct cggcggcgcc gccctcttcg tccccgcgga cgtcaccgac ggggcggggg
     1501 tcgagggggc cgtgggacgc gtcgtatcgg cgttcggccg cctcgacgtc gcggtcacct
     1561 gcgcgggcgt cggaacttcg gggcgcgtcc tcgacaagac gggcgcgccg cacgcgctcg
     1621 acctcttcga gcggatcgtc cgcgtcaacc tcgtcggcac gttcaacgtc ctgcgcatcg
     1681 ccgcgtccgc gatgtaccgc aacgccccga acgccgacgg cgagcggggc gtcgtcgtcg
     1741 cgaccgcctc cgtcgcggcg tacgacggcc aggtgggcca ggtcgcctac gccgcgtcga
     1801 agggaggcgt ggtctcgatg accctccccg tcgcgcgcga cctcgcgcag tgggggatcc
     1861 gctgcgtgac gatcgcgccg gggatcttcg acacgccgat gctcgcgacg gtgaaggaag
     1921 agttccgcgc gaggctcgcg gccggcgtcc ccttcccgag ccgcctcggc gacccggcgg
     1981 agtacgcccg cctcgtcgag cagatcgtcg gaagccccta cctcaacggc gaggtcgtcc
     2041 gcctcgacgg ggcgctccgc atggcgccgc gctgacgaaa cctggggtca ggtcttgtga
     2101 cgaaacctgg cgaaacctgg ggtcaggtct tgtgacgaaa cctggcgaaa cctggggtca
     2161 ggtctnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntgcga tgaggcattc
     2281 gcggctcgcg aatgcctcat cgcaagacct gaccccagcc tcagggctgg cccgtcgccg
     2341 gtgcgcgctt ccagccggac gcgacggctc cgagcgcgga gacgaccgcg cagccgacga
     2401 agagggcgcg gtagccgccg aggtcgatca gggggccgcc gacggcgggg ccgacgaaga
     2461 agccggcctg gaagacggcc atgccgaggt tcgtgttg
//
LOCUS       DAOTEZ010000555         2490 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_34401_length_2490_cov_5.130595, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000555 DAOTEZ010000000
VERSION     DAOTEZ010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34401_length_2490_cov_5.130595"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1673)
                     /locus_tag="P5164_19290"
     CDS             complement(<1..1673)
                     /locus_tag="P5164_19290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46083.1"
                     /translation="MISLASLALASALAAPPERPHLTPWVVVRPEATAPPAAGPAPGS
                     LSANAPGLLVEARAADVGVPATPERVRRLFAEARAAGRRAGLLVELPEVKVPENAREA
                     EAVTPDSLVPGLGALLASAREADLFVLSLPDLSGEVRSRRWLLMKAAAIARSVGPTAR
                     IAVLFHQPAGGGLFPPGAQEILSEEAAAFVDLLGLDLDDASTTPEAVRQAADGLSFAR
                     PLLVTAPQVADAGALVDLAARFSTVDSPVVAAPLADDSGAPLLDRLGALLSGDVSRDG
                     RSATAKTPDGKALSVLRLAKGTDLGGVVLVTSADETGSPARGPFVLELDGPSYTTAEV
                     RELATGRSRSFDLPKSGTPRLSLTTANGPLALVLTAREKAPEEATRASTDVTATRGLT
                     VEEILARHQAWRAGRDARWTRFTAVNRTSYRFRLEGFPTSFELTFEGPFFYERGKGFD
                     WAWQTAYVNGVRWKGKKIPELPLLQPEKVSELPLELTFNEAYRYVLDGEDEVGGVPCW
                     VVAFEPSAGFKAKAIYSGKVYIGKADFSLRRVAAKQLNLTGEVQSVDETT"
     gene            complement(1757..>2490)
                     /locus_tag="P5164_19295"
     CDS             complement(1757..>2490)
                     /locus_tag="P5164_19295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46084.1"
                     /translation="GHSARRSLVKNVLRRTLTGLFGPEGPYEVPVPRPMTLGPLESGQ
                     LLTSNDRDIVQFAVIRAPENAVVRAEVERRQLVSSGCGSGAGSGRSADLGEGRAPLPV
                     FREPFPAGATTIMGPVDLGSPRVVTDCVSGYQQHGSRRVNVTLFNAGGEEAVFLIRSF
                     RMQGDPEPVLEVPVTLGPGRVHQVNGLPIFAAIAESGAPWRPQELAWVTVEATQPFLV
                     YTSTVFIDPEPATLPFQVFPPTRIR"
BASE COUNT          370 a          861 c          932 g          327 t
ORIGIN      
        1 gtcgtctcgt cgaccgactg gacctcgccc gtcaggttca gctgcttcgc ggcgacgcgc
       61 cggagcgaga agtcggcctt gccgatgtaa accttacccg agtagatcgc cttcgccttg
      121 aaaccggcgc tcggctcgaa cgcgacgacc cagcagggga cgccgccgac ctcgtcctcg
      181 ccgtcgagga cgtagcggta cgcctcgttg aacgtcagct cgagcgggag ctcggagacc
      241 ttctcgggct gcagcagcgg cagctcgggg atcttcttcc ccttccaacg cacgccgttg
      301 acgtaggccg tctgccaggc ccagtcgaag cccttcccgc gctcgtagaa gaacggcccc
      361 tcgaacgtca gctcgaagga ggtggggaag ccctcgaggc ggaatcggta cgacgtcctg
      421 ttcacggcgg tgaagcgggt ccagcgggcg tcgcgcccgg cgcgccaggc ctggtgccgc
      481 gcgaggatct cctccaccgt caggccgcgg gtcgcggtga cgtccgtcga ggcgcgggtc
      541 gcctcctccg gcgccttctc gcgggcggtc aggacgagcg ccagcgggcc gttcgcggtc
      601 gtcagcgaga ggcggggcgt tccggacttc ggcaggtcga aggagcgcga gcggccggtc
      661 gcgagctcgc ggacctcggc cgtcgtgtac gaggggccgt ccagctcgag gacgaagggg
      721 ccgcgggcgg gggagccggt ctcgtccgcg ctcgtcacga ggacgacgcc gccgaggtcg
      781 gtccccttcg cgaggcgcag gaccgacagc gccttcccgt ccggggtctt cgccgtcgcg
      841 ctccggccgt cgcggctgac gtcgcccgag aggagcgcgc cgagccggtc gaggagcggg
      901 gcgccggagt cgtccgcgag cggcgccgcg acgaccgggg agtcgacggt cgagaagcgg
      961 gcggcgaggt cgacgagcgc accggcgtcc gcgacctgcg gcgccgtcac gaggagcggg
     1021 cgcgcgaacg agaggccgtc cgcggcctgc ctcaccgctt ccggcgtcgt cgaggcgtcg
     1081 tcgaggtcga ggccgaggag gtcgacgaac gccgccgctt cctcggagag gatctcctgc
     1141 gcccctggcg ggaagagccc gccgcccgcg ggctgatgga agaggacggc gattcgggcc
     1201 gtcggcccga ccgaccgcgc gatcgcggcc gccttcatca ggagccagcg gcgcgagcgg
     1261 acctcgcccg agaggtcggg aagcgagagg acgaagaggt ccgcctcacg ggccgaggcg
     1321 aggagcgcac cgagccccgg cacgagggag tcgggggtca cggcctccgc ctcgcgcgcg
     1381 ttctcgggca ccttcacctc gggcagctcg acgaggagcc cggcccgccg gccggccgcc
     1441 cgcgcctcgg cgaagaggcg gcggacgcgt tccggcgtcg ccggcacgcc cacgtcggcg
     1501 gcccgggcct cgacgaggag gcccggcgcg ttcgcggaga ggcttccggg agccggtccg
     1561 gcggcgggcg gcgccgtcgc ctccgggcgg acgacgaccc agggggtgag gtggggccgc
     1621 tcgggagggg cggcgagggc cgacgcgagc gccagcgagg caaggctgat catggcgcga
     1681 gttatacgca atcgccgggc cgtgcgttcg gaacgtgcgt cacgtcggga cgcgtcccaa
     1741 aaatggaacg ggacgactat cggattcggg tggggggaaa gacctggaac gggagcgtcg
     1801 ccggctcggg atcgatgaag accgtgctcg tgtacacgag gaacggctgg gtggcctcca
     1861 cagtcaccca tgcgagctcc tgtggcctcc agggagcgcc gctctctgcg atcgcagcga
     1921 agatcgggag cccgttgacc tggtggaccc tcccgggacc gagcgtcacg gggacctcca
     1981 gcacgggttc ggggtctccc tgcatgcgaa agctccggat caggaagacg gcctcctcgc
     2041 cgcccgcgtt gaagagtgtc acgtttactc gcctgctacc gtgctgctgg tacccgctga
     2101 cgcagtccgt gacgacgcgc gggctcccca ggtcgaccgg acccatgatc gtcgtcgccc
     2161 ccgccggaaa cggctccctg aagacgggaa gcggagcccg tccctcgccg agatccgcgc
     2221 tgcgtcccga accagctccc gagccacaac cagaagacac caactggcgt cgttcgacct
     2281 ccgcgcggac gaccgcattc tcgggtgccc ggatcacggc gaactgaacg atgtccctgt
     2341 cgtttgacgt caggagctgt ccgctctcga gcgggcccag cgtcatcggt ctcggcactg
     2401 gcacttcgta cggcccctcc ggaccgaaga ggccggtaag cgtccgacga agtacgttct
     2461 tgacgagcga gcgacgggcg gagtggccga
//
LOCUS       DAOTEZ010000556         2482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_34567_length_2482_cov_5.915946, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000556 DAOTEZ010000000
VERSION     DAOTEZ010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34567_length_2482_cov_5.915946"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="P5164_19300"
     CDS             <1..341
                     /locus_tag="P5164_19300"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRY46085.1"
                     /translation="REMVSAGVAVALGTDCNPGSSYTESLPAVAFFATIGMGLTVEEA
                     LTAMTLNAAASVGEAARRGSLEPGKAADVVLVDGRNLEHLVYHYGVNTVTDVVVAGSP
                     VVRDGRRVRA"
     gene            387..1025
                     /locus_tag="P5164_19305"
     CDS             387..1025
                     /locus_tag="P5164_19305"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HRY46086.1"
                     /translation="MTDLVSLAVRDFSAALASDAPAPGGGSASAAAGAMGAALLGMVC
                     RLTLGKEKYRESWEELERVASAMDANRDTLLGLVDEDTRAYDAIVAARKLPKETPEEK
                     AARKKAIDEATILATTVPMQTAFFSMEALAKAPVVLEKGNPNCASDAYVAALLLSSAL
                     EGALANVRINLPGISDASLAEGFRNDAELAAKKAAEAMEKVRALAKAKELAG"
     gene            1295..2086
                     /locus_tag="P5164_19310"
     CDS             1295..2086
                     /locus_tag="P5164_19310"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HRY46087.1"
                     /translation="MPLAGWLTWWRKEEGASFHFREEGGLGRRSYAGGADKASHFVMA
                     AIAQDGAEAIYRSVGKDERTARLLGIALVSTAGLVIEIGDGLTKYGASWEDAAANVLG
                     AAAAAGVNGLRLEDTIGFRFGVVKARVPDPCCRATAYGGDYSRWVHSVDLKLDGLLPR
                     LGVSPGPARFLLVSLTYGSKGYRFSPAEVRQRNVGVDLGLNVEQLLRAAGVRDDRWWG
                     RTLLLLARTYRIPWTAFGWRYDLNSGRWHGPDTGDRFDPGAVIYD"
     gene            complement(2153..>2482)
                     /locus_tag="P5164_19315"
     CDS             complement(2153..>2482)
                     /locus_tag="P5164_19315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017478127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRY46088.1"
                     /translation="SIEAKGKAMAEFNKLVETENTINKIALAKAPVPAAPTPAAGAVV
                     TGGAPAAAAAGAGKVHVVEKGDTLSAIAKKYYGKANAYMKIFEANKDVLTDPDKIKPG
                     QRLRIPD"
BASE COUNT          317 a          917 c          893 g          355 t
ORIGIN      
        1 cgcgcgagat ggtctcggcc ggcgtcgcgg tcgccctcgg gaccgactgc aacccgggct
       61 cctcctacac cgagtcgctc cccgccgtcg ccttcttcgc gacgatcggg atggggctga
      121 cggtcgagga ggcgctcacg gcgatgacgc tcaacgccgc cgcctcggtc ggagaggcgg
      181 cgcgtcgcgg ctcgctcgag ccggggaagg cggcggacgt cgtcctcgtc gacggccgaa
      241 acctcgagca cctcgtctac cactacggcg tcaacaccgt gaccgacgtc gtcgtcgccg
      301 ggagtcccgt cgtccgcgac gggcgccgcg tccgggcctg acggaccggg cgcgcccccc
      361 accaacgaac agagcaggag gaacccgtga ccgacctcgt ctccctcgcc gtccgcgact
      421 tcagcgccgc tctcgcctcc gacgcccccg cccccggcgg cggctcggcc tcggccgcgg
      481 cgggagcgat gggcgccgcc ctcctcggaa tggtctgccg cctcaccctg ggcaaggaga
      541 agtaccgcga gtcgtgggag gagctggagc gcgtcgcctc ggcgatggac gcgaaccgcg
      601 acacgctcct cggcctcgtc gacgaggaca cgcgcgccta cgacgcgatc gtcgccgcgc
      661 ggaagctccc gaaggagacg cccgaggaga aggcggcgcg gaagaaggcg atcgacgagg
      721 cgacgatcct cgcgacgacc gtcccgatgc agaccgcgtt cttctcgatg gaggcgctcg
      781 cgaaggctcc cgtcgtcctc gagaagggga acccgaactg cgcctccgac gcctacgtcg
      841 ccgccctcct cctctcgtcg gcgctcgagg gggcgctcgc gaacgtgagg atcaacctcc
      901 cgggcatctc cgacgccagc ctcgccgagg gcttccgaaa cgacgcggag ctggcggcga
      961 agaaggcggc cgaggcgatg gagaaggtcc gggccctcgc gaaggcgaag gagctcgcgg
     1021 gctgaggagc ctccgccgcc gcggggccgc tctcctcgcc ttcctcctct gtgcggcggc
     1081 tccggctctc ggggccaccg ccggggaggc gaagcccgcc ttccggctcg aaggggcccg
     1141 cctcatcgcg gcgcctctct ccgggcttcc cgctgcttct ccgcttccgg gacccgggga
     1201 gagcccggct tcgggggccg ccgcgccgac ggcccccccg gaaaggctcc tcgacgcccg
     1261 gaccgcgcgg gtctcggcgg ccgtcctcgc ggcggtgccg ctcgccgggt ggctcacctg
     1321 gtggcggaaa gaggaaggcg cgtccttcca cttccgggaa gagggggggc tcgggcgccg
     1381 cagctacgcc ggcggcgcgg acaaggcgtc gcacttcgtg atggcggcga tcgcgcagga
     1441 tggcgcggaa gcgatctatc gatccgtcgg gaaagacgag cggacggcgc gcctcctcgg
     1501 aatcgccctc gtgtcgaccg cgggcctcgt catcgagatc ggcgacggtc tcacgaagta
     1561 cggcgcctcc tgggaggacg ccgcggcgaa cgtcctcggc gcggccgccg ccgcgggcgt
     1621 gaacggtctc cggctcgagg acacgatcgg cttccgcttc ggcgtcgtga aggcacgggt
     1681 ccccgacccg tgctgccggg cgacggccta cggcggcgac tactcccgct gggttcactc
     1741 cgtcgacctg aagctcgacg gtctgctgcc gcgcctcggc gtctcgcccg gcccggcgcg
     1801 cttcctcctc gtctcgctca cctacggctc gaaggggtac cgcttctctc ccgccgaggt
     1861 gaggcagcga aacgtcgggg tcgatctcgg cctgaacgtc gagcagctcc tccgggcggc
     1921 gggagtccgg gacgacaggt ggtggggccg gacgctcctc ctcctggccc gcacctaccg
     1981 gattccctgg acggccttcg gctggcggta cgacctgaac tcgggtcggt ggcacggacc
     2041 cgacaccggc gaccgattcg acccgggcgc agtgatctac gactgacgcg gcgccgcgaa
     2101 cgggcggccg ggccgggggg cggaagcccg cggcccggcc tgagcccggc gatcagtccg
     2161 ggatgcggag ccgctgcccc ggcttgatct tgtccgggtc cgtcaggacg tccttgttcg
     2221 cctcgaagat cttcatgtag gcgttcgcct tgccgtaata cttcttggcg atcgcgctca
     2281 gcgtgtcgcc cttctcgacg acgtggacct tcccggcgcc ggcggcggcc gccgcggggg
     2341 ccccgccggt cacgacggcg ccggcggcgg gcgtcggcgc ggcggggacg ggcgccttcg
     2401 cgagcgcgat cttgttgatg gtgttctcgg tctcgaccag cttgttgaac tcggccatgg
     2461 ccttgccctt ggcctcgatg ct
//
LOCUS       DAOTEZ010000557         2482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_34568_length_2482_cov_5.760198, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000557 DAOTEZ010000000
VERSION     DAOTEZ010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34568_length_2482_cov_5.760198"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="P5164_19320"
     CDS             complement(<1..475)
                     /locus_tag="P5164_19320"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY46089.1"
                     /translation="MPETPGRLTGKKALVTGASRGIGRAIALRLAAEGATVACGVRSE
                     ADGAALVAEIAAAGGAAVPVLLDVTDAASVATGVERAARPDRRLDVVVNNAGYTSPTP
                     LSGDERSDQAFERSWEVNVAGTWRVLRAALPFLAEGGRIVNLSSVLGRFGAPGQAG"
     gene            533..787
                     /gene="moaD"
                     /locus_tag="P5164_19325"
     CDS             533..787
                     /gene="moaD"
                     /locus_tag="P5164_19325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018947155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HRY46090.1"
                     /translation="MKVRILFFASLRDELGDSRSVEAVEALPGETTVAALREAVAALS
                     PAAARLGRRLLVAVNERYATDGDPVRPGDSVAFLPPLAGG"
     gene            790..1209
                     /locus_tag="P5164_19330"
     CDS             790..1209
                     /locus_tag="P5164_19330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HRY46091.1"
                     /translation="MPHLSREPLDPGALLEAARRDGDGGLALFVGVVRDNNDGRRVTE
                     MEYEAYEPMAEAEMERIEAELAGRFPVVRVLLRHRIGRLRIGEVAVVVAASAPHRDEA
                     FAACRAGIEEIKARVPVWKRERGPDGSVWVDPGAPLG"
     gene            1341..1985
                     /locus_tag="P5164_19335"
     CDS             1341..1985
                     /locus_tag="P5164_19335"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRY46092.1"
                     /translation="MFVALRRVHVAASDFPRSRRFYGETLGLPEVGGFESDWVEFGLG
                     PILVKVTPLRAGEVSARNQVALMLSTGDIEACLTTLKERGVTVTRGPLEEFTGRSAEI
                     LDPDGYRIVVFQPSDLHPDEEWVPMSEVDEKVKEKIAAWRAKEKTKAAAAPKRSVPPA
                     AKAAPKKAAPKKPASRKPAPKKPAPKKAVRPAPKKTAPKKAAPKKPAPKKAKKK"
     gene            2055..>2482
                     /locus_tag="P5164_19340"
     CDS             2055..>2482
                     /locus_tag="P5164_19340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020469451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRY46093.1"
                     /translation="MSTALIDYCTSEIEALKEARTFKKERVLEGPTGARVRVDGREVL
                     MLTSNNYLGFANHPRVVEAARKALERWGNGLGSVRFICGTQELHKELERTISSFFGTQ
                     DTILYMSCWNANEGLFQPLLGEEDAILTDGLNHASIIDGIR"
BASE COUNT          379 a          922 c          839 g          342 t
ORIGIN      
        1 aacccgcctg cccgggcgcg ccgaagcgcc ccagcaccga cgacaggttc acgatccgcc
       61 ctccctcggc gaggaacggc agggcggccc gaaggacgcg ccacgtgccg gccacgttca
      121 cctcccacga ccgctcgaag gcctggtcgg agcgctcgtc cccggagagc ggcgtcggcg
      181 acgtgtagcc ggcgttgttg acgacgacgt cgaggcgccg gtccggccgc gccgcccgct
      241 cgacgcccgt cgcgaccgag gccgcgtccg tcacgtcgag gaggaccggg accgccgcgc
      301 cgccggccgc cgcgatctcg gcgacgagcg cggcgccgtc ggcctcggag cgaacgccgc
      361 aggcgacggt cgccccttcc gccgcgaggc ggagggcgat cgcccggccg atgccgcgcg
      421 acgcccccgt cacgagagcc ttcttccccg tcagccgtcc cggtgtctcg ggcatggaac
      481 ctcctgattc ggcgggcatt ctagaactcc gccgacgtta agatccggac cgatgaaggt
      541 ccggatcctc ttcttcgcgt ccctccggga cgagctcggc gattcgcgga gcgtcgaggc
      601 cgtcgaggcc cttcccggcg agacgaccgt cgccgcgctc cgcgaggccg tcgcggccct
      661 ctcccccgcc gcggctcggc tcgggcgccg gctcctcgtc gccgtgaacg agcggtacgc
      721 cacggacggc gacccggtcc gtccgggcga ttccgtcgcc ttcctccccc ctttggccgg
      781 aggctgagcg tgccccacct ttcccgcgag cccctcgatc ccggcgccct cctggaggcc
      841 gcccgtcggg acggcgacgg aggcctcgcc ctcttcgtcg gggtcgtccg cgacaacaac
      901 gacgggcgtc gcgtcaccga gatggagtac gaggcctacg agccgatggc cgaggccgag
      961 atggaacgga tcgaggcgga actggcaggg cgctttcctg tcgtccgcgt ccttctccgg
     1021 catcggatcg gtcggctccg gatcggggag gtcgccgtcg tcgtcgccgc ctcggccccc
     1081 catcgggacg aggccttcgc ggcctgccgc gcgggaatcg aggagatcaa ggcacgggtc
     1141 cccgtctgga agcgggagcg gggcccggac ggaagcgtct gggtggaccc tggagcgcct
     1201 ctcggataag cggaaagttg ttgaaaagtc aaccactttc tggtaaacgg gcgcttcagg
     1261 ggaacgctcc ccggatttcg tccgtgtcac cggcggcgga tctccttccc ggccgccggg
     1321 aaaccaatcg ggaggttgag atgttcgttg ccctccggcg ggtgcacgtc gccgccagcg
     1381 actttccgcg gtctcgccgt ttctacgggg aaacgctcgg ccttcccgag gtcgggggct
     1441 tcgagagcga ctgggtcgag ttcgggctcg gaccgatcct cgtcaaggtc accccgctcc
     1501 gcgcgggcga ggtctcggcc cgcaatcagg tcgccctcat gctctcgacg ggggacatcg
     1561 aggcctgcct gacgaccctc aaggagcgcg gcgtcaccgt gacccggggc cccctcgagg
     1621 agttcaccgg gcgctcggcg gagatcctcg atcccgacgg ctaccggatc gtcgtcttcc
     1681 agccttccga ccttcacccc gacgaggagt gggtcccgat gtcggaggtc gacgagaagg
     1741 tgaaggagaa gatcgcggcc tggagggcca aggagaagac caaggccgcc gccgcgccga
     1801 agcggtccgt tccgcccgcc gcgaaggcgg ccccgaagaa ggcggccccg aagaagcccg
     1861 catcccgcaa gccggctccg aagaagcccg ccccgaagaa ggccgtccgg cccgctccga
     1921 agaagacggc cccgaagaaa gcggccccga agaagcccgc cccgaagaag gcgaagaaga
     1981 agtagctcgt tcgcccccgg gaaggccgtt ccgagcgccc cgctccccgc ggggcgctgg
     2041 tattctggcc ggtcatgagc accgccctca tcgactactg cacgtccgag atcgaggccc
     2101 tgaaggaggc ccgcaccttc aagaaggagc gcgtcctcga ggggcccacc ggggcccgcg
     2161 tccgcgtcga cggccgcgag gtcctgatgc tcacctccaa caactacctc ggcttcgcga
     2221 accacccccg ggtcgtcgag gcggcccgca aggccctcga gcgctggggc aacggcctcg
     2281 gctccgtccg cttcatctgc ggcacccagg agctccacaa agagctcgag cggacgatct
     2341 cctcgttctt cggcacccag gacacgatcc tctacatgtc gtgctggaac gcgaacgagg
     2401 ggctcttcca gccgctcctc ggcgaggagg acgccatcct gacggacggc ctcaaccacg
     2461 cctccatcat cgacggcatc cg
//
LOCUS       DAOTEZ010000558         2465 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_34938_length_2465_cov_5.217427, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000558 DAOTEZ010000000
VERSION     DAOTEZ010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34938_length_2465_cov_5.217427"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="P5164_19345"
     CDS             complement(<1..1430)
                     /locus_tag="P5164_19345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY46094.1"
                     /translation="MTQAPAPADPGLVLLTGATGYVGGRLLRALAARGRRVRCLARRP
                     EALASRVPAGTEVVSGDCLKRETLDPALAGVDAAYYLVHSMAAGAEFEEQDRLAARNF
                     GEAARAAGVRKIVYLGGLGEAGEGLSAHLRSRQETGEVLRSASGLPVVELRASIVLGS
                     GSLSFELIRSLVERLPAMICPRWVETKAQPIAIEDLVAYLLAALDVDPGEGLTVEIGG
                     ADQVTYGDLMREYARQRGLKRWLIPVPVLTPRLSSLWLGLTTPVYARVGRKLIEGVRN
                     PTVVRDDAARRLFPAIVPMGLEKSMERALANEDEEFAATRWSDALSSAGTAPGGYGGL
                     RVGQRLVDSRTARVATTAAQAFAPIRRIGGESGWYYASFLWRLRGFLDLLVGGVGVRR
                     GRRHPDLLAVGDALDFWRVEAIEPDRRLRLAAEMKLPGRAWLEFEVREDEGGSTIRQT
                     ATFDPAGLAGLLYWYGIYPLHVLVFRG"
     gene            complement(1427..2233)
                     /locus_tag="P5164_19350"
     CDS             complement(1427..2233)
                     /locus_tag="P5164_19350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HRY46095.1"
                     /translation="MNLALLAGNAALVLAMMTVLWIVSVRLRDASIVDPWWSTAFLLV
                     AANTVLRTGLTPGKTLLLATVAAWALRLWLHLLLRSLGQPEDPRYAGFRAKYGPERYW
                     WFSFFQVFLLQAVLAVVISAPLQVAGAAAAPDPVSITDLLGLALFAAGFGIEAVADAQ
                     LTRYRKARASGGPGAPGPVLDTGLWRYSRHPNYFGEALLWWGFWLFALDIPWGWATVF
                     APALMTFLLVKVSGVAMLDAHMAAKRPEYAAYIGRTSGFLPLPPRGRSAA"
     gene            complement(2230..>2465)
                     /locus_tag="P5164_19355"
     CDS             complement(2230..>2465)
                     /locus_tag="P5164_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007248624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HRY46096.1"
                     /translation="AVTVAWAVLGARSKGDAARRGAGLGFVAYATYDLTNWAVIRDWP
                     ALLVPVDLAWGVVLTTLAALAGRAAHDAVRGNR"
BASE COUNT          365 a          841 c          988 g          271 t
ORIGIN      
        1 ccccggaaga cgaggacgtg gagcgggtag atgccgtacc agtagaggag gccggcgagc
       61 ccggcggggt cgaacgtggc ggtctggcgg atcgtcgagc ctccttcgtc ctcccgcacc
      121 tcgaactcga gccacgcccg tccggggagc ttcatctcgg cggcgaggcg cagccgccgg
      181 tcgggctcga tcgcctcgac ccgccagaag tcgagcgcgt cgccgacggc gaggaggtcc
      241 gggtggcgcc ggccccggcg gacgccgacg ccgccgacga ggaggtcgag aaagccgcgg
      301 agccgccaga ggaagctggc gtagtaccag ccgctttcgc cgccgatccg gcggatcggc
      361 gcgaaggcct gcgccgccgt cgtcgcgacc cgcgccgtgc gcgagtcgac gagcctctgt
      421 ccgacccgca gccctccgta gccgccgggc gccgtgccgg ccgaggagag ggcgtcggac
      481 cagcgcgtcg cggcgaactc ctcgtcctcg ttcgcgaggg cgcgctccat ggacttctcg
      541 aggcccatcg ggacgatggc ggggaagagg cggcgggcgg cgtcgtccct cacgaccgtc
      601 gggttgcgga ccccctcgat cagcttgcgc cccacgcgcg cgtagaccgg cgtcgtcagg
      661 ccgagccaga ggctcgagag gcggggcgtc aggacgggca ccgggatcag ccagcgcttc
      721 agtccgcgct gcctcgcgta ttcccgcatc aggtcgccgt acgtcacctg gtcggcgccg
      781 ccgatttcca cggtgagccc ctcgccgggg tcgacgtcga gcgcggcgag gaggtaggcg
      841 acgaggtcct cgatcgcgat cggctgggcc ttcgtctcca cccagcgggg gcagatcatg
      901 gcggggaggc gttcgacgag cgaccggatg agctcgaacg agaggctccc cgagccgagg
      961 acgatcgagg cgcgcagctc gacgaccggg aggccggagg cggaccggag cacctcgccc
     1021 gtctcctgcc ggctgcgcag gtgggccgag aggccctctc cggcctcgcc gaggccgccg
     1081 aggtagacga tctttcgcac gcccgcggcg cgggccgcct cgccgaagtt gcgcgcagcg
     1141 aggcggtcct gctcctcgaa ctccgcgccc gcggccatcg agtggacgag gtagtacgcg
     1201 gcgtcgacgc cggcgagcgc cgggtcgagc gtctcgcgct tcaggcagtc gcccgaaacg
     1261 acctcggtcc ccgcggggac gcgggacgcg agggcttccg gccgtcgcgc gaggcagcgg
     1321 acgcgccggc cgcgcgccgc gagcgcgcgc aggagccggc cgccgacgta gcccgtcgcg
     1381 ccggtgagga ggacgaggcc ggggtcggcg ggggcagggg cttgcgtcac gcggcgcttc
     1441 gcccgcgcgg cgggagcggg aggaaccccg aggtccgacc gatgtaggcg gcgtactcgg
     1501 gccgcttcgc ggccatgtgg gcgtcgagca tcgcgacgcc cgagaccttc acgaggagga
     1561 acgtcatgag cgcaggggcg aagacggtgg cccaacccca cggaatgtcg agcgcgaaga
     1621 gccagaagcc ccaccagagg agcgcttctc cgaagtagtt cgggtggcgg ctgtaacgcc
     1681 agagcccggt gtcgaggacc gggccgggcg cgcccgggcc gccggaggcg cgcgccttgc
     1741 ggtagcgggt gagctgggcg tcggcgacgg cctcgatccc gaagcccgcg gcgaagaggg
     1801 cgaggccgag gaggtcggtg atcgagaccg ggtcgggcgc ggcggcggct ccggcgacct
     1861 gcagcggcgc ggagatgacg acggcgagga cggcctggag gaggaagacc tggaagaagc
     1921 tgaaccacca gtagcgctcc ggcccgtact tcgcgcggaa cccggcatag cgcgggtcct
     1981 cgggctgtcc gaggctgcgg agaaggaggt ggagccagag acggagcgcc caggcggcga
     2041 cggtggcgag gaggagcgtc ttccccggcg tgagccccgt ccggaggacg gtgttcgcgg
     2101 cgacgaggag gaacgccgtc gaccaccacg ggtcgacgat gctggcgtcg cgcaggcgga
     2161 cgctgacgat ccagaggacc gtcatcatcg cgaggacgag cgcggcgttg ccggcgagga
     2221 gcgcgaggtt caccgattcc cccggacggc gtcgtgcgca gcgcggccgg cgagagcggc
     2281 gagggtcgtc aggacgacgc cccaggcgag gtcgaccggg acgaggagcg ccggccagtc
     2341 gcggatcacc gcccagttcg tcaggtcgta ggtcgcgtag gcgacgaacc cgaggccggc
     2401 gccgcgccgg gcggcgtccc ccttcgaccg ggcgccgagg acggcccagg cgacggtgac
     2461 ggcga
//
LOCUS       DAOTEZ010000559         2463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_34986_length_2463_cov_3.425664, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000559 DAOTEZ010000000
VERSION     DAOTEZ010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34986_length_2463_cov_3.425664"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="P5164_19360"
     CDS             complement(<1..433)
                     /locus_tag="P5164_19360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46097.1"
                     /translation="MVAPELIDPAALLRGPDLEFGVRKGDRFEIRVSGSGWTYLGEST
                     GQEGIAYEHRRFQDGDAVFALSADRAGDYLLDFRRLDPLLGVPESKVARIVVADAAVP
                     IGAPSSGLGTGIMGSVTGSRIAAPSPVTPSAGSVPAAASGVV"
     gene            complement(1045..1578)
                     /locus_tag="P5164_19365"
     CDS             complement(1045..1578)
                     /locus_tag="P5164_19365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46098.1"
                     /translation="MLAAAGRYAKEVRARFSKDAKAPPPANDDDAAVSALGYDDDAAH
                     VAADALKSVRVKPGELLKEGVSGLRERPVLLFALGLFIAFVLALALVFALAARPPRPV
                     EAPALPEKRWLELAGRLEPPEPMPDAAVFPLDRPRRSVFSEEDLLLLMPDLEWVDTRD
                     IESRVREKVDTMLDGLE"
     gene            complement(1618..>2463)
                     /locus_tag="P5164_19370"
     CDS             complement(1618..>2463)
                     /locus_tag="P5164_19370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRY46099.1"
                     /translation="NLMAVEEYAEVKERYDFLSTQLADLEKAREDLKRITAEIRAESA
                     ELFLETYNKIKKNFHNLFRRLFGGGRGELRMVDPDHVLESGIEIYAQPPGKRLEAISL
                     LSGGEKSMTAIGLLFATYMVKPSPFCFLDEIDAALDEQNVVRFVTLLREFGRMSQFIV
                     ITHNKKTVTGADALLGVTMEESGVTKAIAMRLERSDGAPIVPPKPIVAMEDEEEVPWE
                     DGRELSLGLDDAASAPGIADGAFPDPVSEPLPDPETGTEPREADIGSDGNDEDADSAD
                     GEGQS"
BASE COUNT          398 a          763 c          937 g          365 t
ORIGIN      
        1 gaaccacgcc cgaagccgcg gcggggacag agcccgcgct tggagtgaca ggcgagggcg
       61 cggcgatccg gctgcccgtg acgctgccca tgatgccggt acccaggcca ctcgaaggag
      121 caccgatcgg tacagccgcg tccgccacca cgatgcgggc caccttcgat tcgggcaccc
      181 cgagaagcgg atcgagcctg cggaaatcga gcaggtaatc gcccgcgcga tcggcgctca
      241 aggcgaagac cgcgtcgccg tcctgaaagc ggcgatgctc gtaggctatg ccctcctgcc
      301 ccgtagactc accgaggtag gtccaacccg atccggatac gcggatttcg aagcgatcgc
      361 ctttacggac gccgaattcc agatcgggcc cccggagaag cgccgcggga tcgataagct
      421 cgggcgcgac catgggtatc acggcaatcg aggtcgagtc cggagctggg gacagggctg
      481 gcgccgggga cggagcgggt gcagaggacg gagcgggtgc agaggacgga gtgggcgcag
      541 aggacggagc ggcggccgga gacggagcgg gcgcagagga cggagcggga gccggagacg
      601 gagcgggcgc aggggacagg gctggggccg gggacggagc gggcacgggg gtaggcttaa
      661 gctcaggagg ttttgccgtc gggggagtcg gagccggctt cggcgtttcg acgggaggca
      721 cggcggattc gggcgcagcg ggggcgaccg gaaccggagc ggcgggagca ggcacgggag
      781 cggcgggctc gtagagaggc acgccgacag ggtcgaccgc ctgtggggtc gcgaggtagg
      841 tgaccgccgg tcccatttca ggcaccggtg cgcccgggaa ggcctcggaa accggatcga
      901 tggcgttgac ttcggcaacc ggctccgtct cagggccggt cggctccgga gcggtcgcgc
      961 aggacgcgag gccgaacgag acgccgagcg cgagcagcgg ccagatccag cgtatcccag
     1021 cgtccccgcg tctcggcata tcggctactc cagaccgtcc agcatcgtat cgactttctc
     1081 gcgcactcgc gactcgatat cccgagtatc gacccattcc aggtccggca tgaggaggag
     1141 gaggtcctcc tcggagaaga ccgatcgtcg ggggcggtcg agcgggaaca cggcggcgtc
     1201 cggcatgggc tcgggcggct cgaggcggcc ggccagttcg agccagcgct tttcggggag
     1261 cgccggcgcc tctacgggtc gaggggggcg ggcggcgagc gcgaaaacga gggcgagagc
     1321 caatacgaag gcaatgaaga ggcccaaggc gaacaggaga accggtcgct ccctgagtcc
     1381 cgaaacgcct tccttgagga gctcgcccgg tttcacgcgg acgctcttga gggcgtccgc
     1441 ggcgacatgg gccgcgtcgt cgtcgtatcc gagcgcggag accgccgcgt cgtcgtcatt
     1501 cgccggaggc ggggctttcg cgtccttcga gaagcgggcg cggacttcct tcgcgtagcg
     1561 tcccgccgcc gcgagcaatt tcgcgggtcc gttccccgaa ggagccttgc cggccatcta
     1621 gctctgtccc tctccgtcgg cggagtcggc gtcttcgtcg ttcccgtcgg atccgatatc
     1681 ggcctcacgg ggctccgtgc cggtttccgg gtcgggaagc ggctccgaca cggggtccgg
     1741 gaaagcgccg tccgcgatgc ccggggccga tgcggcgtcg tcgaggccga gcgagagctc
     1801 gcggccgtct tcccagggca cctcctcctc gtcctccatg gccacgatgg gcttgggcgg
     1861 gacgatgggc gcgccgtcgg agcgctcgag gcgcatggcg atggccttgg tgacgccgga
     1921 ttcctccatg gtcacgccga gcagggcgtc ggcgccggtc accgtcttct tgttgtgcgt
     1981 gatgacgatg aactggctca tgcggccgaa ctcgcggagc aaggtgacga agcgcacgac
     2041 gttctgctcg tcgagggccg cgtcgatctc gtcgaggaag cagaagggcg aaggcttgac
     2101 catgtacgtg gcgaagagga ggccgatggc ggtcatcgac ttctcgccgc ccgaaagcag
     2161 gctgatggcc tcgaggcgct tcccgggcgg ctgggcgtag atctcgatgc ccgactcgag
     2221 cacgtggtca gggtcgacca tgcggagctc gccgcggccg ccgccgaaga gccggcggaa
     2281 gaggttgtgg aagttcttct tgatcttgtt gtaggtctcg aggaagagct cggcgctctc
     2341 ggcgcggatc tccgcggtga tgcgcttcag gtcctcgcgc gccttctcga ggtccgcgag
     2401 ctgggtcgag aggaagtcgt agcgctcctt gacctcggcg tactcctcga cggccatcag
     2461 gtt
//
LOCUS       DAOTEZ010000560         2458 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_35095_length_2458_cov_6.786933, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000560 DAOTEZ010000000
VERSION     DAOTEZ010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35095_length_2458_cov_6.786933"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..462)
                     /locus_tag="P5164_19375"
     CDS             complement(112..462)
                     /locus_tag="P5164_19375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRY46100.1"
                     /translation="MDVRETPAWRKAIGLAHEVCATLEDAADLPGDSGQRLRRAALAV
                     PSLVADAFLDTGPADCRERLSAAAARVAELRQLLRAPETRLFVPGPEADALLAEAESL
                     ALEIRALGGRCPEA"
     gene            610..1536
                     /gene="mdh"
                     /locus_tag="P5164_19380"
     CDS             610..1536
                     /gene="mdh"
                     /locus_tag="P5164_19380"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRY46101.1"
                     /translation="MRRKITVIGAGNVGATAAQRIVEKELGDVVLVDVVDGVPQGKGL
                     DMYQSGAVEGFDMKIVGSNGYEETADSDLCIMTAGLPRKPGMSRDDLLKTNEAIVADC
                     IAKVTRYSRDLTIIVVTNPLDAMCEVARRVSKLPKNKVIGMAGILDSARMRAFIAMEM
                     NVAMSNIHATVLGGHGDTMVPLPRYSTVAGVPITELLPPEKVEEIVKRTANGGAEIVK
                     LLKTGSAYYAPSAAAVEMADAIFNDKKKILPCAALLEGEYGISGLFVGVPVVLGKDGV
                     EKIIELKLTPEEAAALRKSADSVAELCGMLTV"
     gene            1544..2212
                     /locus_tag="P5164_19385"
     CDS             1544..2212
                     /locus_tag="P5164_19385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HRY46102.1"
                     /translation="MSAIPSTAERPVFGSITLKAVMALTGIVLYGFVFVHMVGNIQLY
                     QGPEKINGYAAFLKAVPPLLWGTRITLLAAVVLHGLAAFTLWRRNLAARPVGYAHQDF
                     QAAGVTSRTMYWTGPVIALFVIYHLLHFTLGSAHPQFSHTDVYTNVVVGFSNVWVSGF
                     YVLAMVALGFHLFHGGFSLFQTLGLRTPKYEKPIKAVLAVLCTVIVVVNVSFPIAVLA
                     GLVK"
     gene            2224..>2458
                     /locus_tag="P5164_19390"
     CDS             2224..>2458
                     /locus_tag="P5164_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012929488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HRY46103.1"
                     /translation="MELDGKCPTGPMESRWTRHKAAIKLINPANKRKYSLIMVGSGLA
                     GASGAASLAELGYNVSCFCYQDSPRRAHSIAAQG"
BASE COUNT          400 a          866 c          819 g          373 t
ORIGIN      
        1 ccgtgaggag gacgagggcc gccaggatcg cggcgagcgg gcggaacgtc atcggatctc
       61 ctttccttcc agcgggggaa cgctagcagg aggaggggaa atcgggaagg gtcaggcctc
      121 ggggcagcgt ccgccgagcg cccggatctc gagggccaac gactcggcct cggccagcag
      181 ggcgtcggct tccggcccgg ggacgaagag ccgggtctcg ggcgcccgca ggagctggcg
      241 gagctcggcg acccgggcgg cggcggcgct cagacgctcc cggcagtcgg cgggcccggt
      301 gtcgaggaag gcgtcggcga cgagagacgg cacggccagc gcggcgcgcc ggaggcgctg
      361 ccccgaatcg ccggggaggt ccgccgcgtc ctcgagcgtc gcgcagacct cgtgggcgag
      421 gccgatggcc ttccgccagg caggggtctc ccgaacgtcc atcttcaacc tgaagcatat
      481 acggacgagg gggcccggtc gttccgttcg ggactcccct ttcgggggct tcccccccct
      541 gctacgctta ccggtcggat cggtacagac ccggcggaga ccggaatctt cgagagcagg
      601 gggagaagca tgcgacggaa gatcactgtc atcggcgccg ggaacgtcgg cgcgaccgcc
      661 gcccagagga tcgtcgagaa ggagctcgga gacgtcgtcc tcgtggacgt cgtcgacggc
      721 gtgcctcagg gcaagggtct cgacatgtac cagtcgggcg ccgtcgaagg cttcgacatg
      781 aagatcgtcg ggtcgaacgg ctacgaggag acggccgact ccgacctctg catcatgacg
      841 gcgggcctcc cccgcaagcc cgggatgtcc cgtgacgacc tcctgaagac gaatgaggcg
      901 atcgtcgccg actgcatcgc caaggtgacg cgctactccc gcgacctgac gattatcgtc
      961 gtcacgaacc ccctcgacgc gatgtgcgag gtcgcccgcc gcgtctcgaa gctcccgaag
     1021 aacaaggtca tcggcatggc cggcatcctc gactcggccc ggatgcgcgc cttcatcgcg
     1081 atggagatga acgtcgcgat gtcgaacatc cacgcgacgg tcctcggcgg gcacggcgac
     1141 acgatggtcc cgctcccgcg ctactccacg gtcgccggcg ttccgatcac cgagctgctg
     1201 ccgcccgaga aggtcgagga gatcgtgaag cggaccgcca acggcggcgc cgagatcgtc
     1261 aagctcctga agaccggctc ggcctactac gccccgtcgg ccgcggccgt cgagatggcc
     1321 gacgcgatct tcaacgacaa gaagaagatc ctcccctgcg cggcgctcct cgagggggag
     1381 tacggtatca gcggcctctt cgtcggcgtc ccggtcgtcc tcggcaagga cggcgtcgag
     1441 aagatcatcg agctgaagct gacgcccgaa gaggccgcgg ccctccggaa gtcggccgac
     1501 tccgtcgccg agctctgcgg gatgctgacc gtctgatcga acgatgagcg cgatcccgtc
     1561 cacggcggag agaccggtct tcggctccat caccctcaag gcggtgatgg ccctgaccgg
     1621 catcgtcctc tacggcttcg tcttcgtcca catggtcggc aacatccagc tttaccaggg
     1681 cccggagaag atcaacggct acgcggcgtt cctcaaggcg gtcccgccgc tcctctgggg
     1741 gacccggatc accctcctcg cggccgtggt gctccacggc ctcgcggcgt tcacgctctg
     1801 gcggcgcaac ctcgcggccc gcccggtggg ctatgcccac caggacttcc aggcggccgg
     1861 ggtcacctcc cggacgatgt actggaccgg gccggtgatc gccctcttcg tgatctacca
     1921 cctcctccac ttcacgctcg gctcggccca cccgcagttc agccacaccg acgtctacac
     1981 gaacgtcgtc gtcggcttct cgaacgtctg ggtgtcgggc ttctacgtcc tcgcgatggt
     2041 cgccctcggc ttccacctct tccacggcgg cttcagcctc ttccagacgc tgggcctccg
     2101 gaccccgaag tacgagaagc cgatcaaggc ggtcctcgcc gttctctgca ccgtgatcgt
     2161 cgtcgtgaac gtctccttcc cgatcgcggt cctcgcgggc ctggtgaagt aggaggaagc
     2221 gggatggaac tcgacggcaa gtgccccacc ggccccatgg agtcgcgctg gacgcggcac
     2281 aaggcggcca tcaagctgat caacccggcc aacaagagga agtacagcct catcatggtc
     2341 ggctccgggc tcgcgggcgc ctccggcgcc gcgtcgctcg ccgagctcgg ctacaacgtc
     2401 tcctgcttct gctaccagga cagcccccgc cgcgcgcact ccatcgccgc gcagggcg
//
LOCUS       DAOTEZ010000561         2453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_35203_length_2453_cov_6.815263, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000561 DAOTEZ010000000
VERSION     DAOTEZ010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35203_length_2453_cov_6.815263"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1766)
                     /gene="pnp"
                     /locus_tag="P5164_19395"
     CDS             complement(<1..1766)
                     /gene="pnp"
                     /locus_tag="P5164_19395"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRY46104.1"
                     /translation="MKESVTALVGGRALTIENGKVAKQADGSAVVRLGDTMVLVTACY
                     ARDPKDVDFVPLTVEYKEYQYAAGRIPGGFFKREGRPTEKEILTCRMIDRPIRPLFPA
                     GYAFETQVVALVLSADGENDPDVLAINGTAAALAVNPIPFTEVLGAVRVGRIGDRFVF
                     NPTKKEREESVMDLVVVGTRQAVSMVEAGAKECSEELMLEAILEGHRELQKVIDAIEE
                     LQRRQGVVKQAFTPPAPVPAEVMAEVRRNWDGPMMEALTWKGKIESYAKIKEVKKAAV
                     SVVPDDQPEKKAQVKRAVSELVEVMTRETILRDRKRLDGRAFDEVRPIEVEIGVLPRT
                     HGSALFTRGETQALVTVTLGTSDDTQLIEDLEGEFEKKFLLHYNFPPFSVGEVKRFGS
                     PGRREIGHGRLAWRSIDAVLPKEFPYTIRVVSDILESNGSSSMATICGGSLALMDAGV
                     PMESAVAGVAMGLVAEGDQWAVLTDIAGQEDHYGDMDFKVAGTRKGVTALQMDIKVSG
                     ISREVFEKALAQAKAGRMHILDIMEKAIPTARPEISAYAPRLLTIHVPEEKIRDVIGS
                     GGKTIRALQKEYTVKIDVQDDGS"
     gene            complement(1822..2079)
                     /gene="rpsO"
                     /locus_tag="P5164_19400"
     CDS             complement(1822..2079)
                     /gene="rpsO"
                     /locus_tag="P5164_19400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRY46105.1"
                     /translation="MTPKTETIKAFERHEGDTGSPEVQVALLSQRITALTEHFKTHAK
                     DHHSRRGLLQMVGQRRRLLDYLKKRDVTRYKALIERLGLRK"
BASE COUNT          332 a          805 c          874 g          442 t
ORIGIN      
        1 gagccgtcgt cctggacgtc gatcttgacc gtgtactcct tctggagcgc ccggatcgtc
       61 ttgccgccgg agccgatgac gtcgcggatc ttctcctcgg ggacgtggat cgtcaggagg
      121 cgcggcgcgt aggccgagat ctcggggcgg gccgtcggga tcgccttctc catgatgtcg
      181 aggatgtgca tccggccggc cttcgcctga gcgagcgcct tctcgaagac ctcgcggctg
      241 atgccggaga ccttgatgtc catctggagg gcggtgaccc ctttgcgcgt cccggcgacc
      301 ttgaagtcca tgtcgccgta gtggtcttcc tggccggcga tgtcggtcag gacggcccac
      361 tgatcgcctt ccgcgacgag gcccatcgcg acccccgcga cggccgactc catcgggacg
      421 ccggcgtcca tgagggcgag ggagccgccg cagatcgtcg ccatcgagga ggagccgttc
      481 gactcgagga tgtcggagac gacccggatc gtgtagggga actccttcgg caggacggcg
      541 tcgatggagc gccaggcgag gcggccgtgg ccgatctcgc gccggccggg ggagccgaag
      601 cgcttcacct cgccgacgga gaacggcggg aagttgtagt ggaggaggaa cttcttctcg
      661 aactcgccct cgaggtcctc gatgagctgc gtgtcgtccg aggtcccgag cgtgaccgtg
      721 acgagcgcct gcgtctcgcc gcgcgtgaag agcgcggagc cgtgggtgcg ggggaggacg
      781 ccgatctcga cctcgatggg gcggacctcg tcgaacgcgc ggccgtcgag gcgcttgcgg
      841 tcgcggagga tcgtctcgcg cgtcatcacc tcgacgagct cggagaccgc ccgcttgacc
      901 tgggccttct tctccggctg gtcgtccggc acgacggaga cggcggcctt cttgacctcc
      961 ttgatcttcg cgtacgactc gatcttcccc ttccaggtga gggcctccat catcgggccg
     1021 tcccagttgc ggcggacctc ggccatgacc tcggccggga ccggcgcggg aggcgtgaag
     1081 gcctgcttga cgacgccctg gcggcgctgg agctcctcga tcgcgtcgat gaccttctgg
     1141 agctcgcggt gcccttcgag gatcgcctcg agcatcagct cctcggagca ctccttcgcg
     1201 cccgcctcga ccatcgagac ggcctggcgg gtgccgacga cgacgaggtc catcacggac
     1261 tcctcgcgct ccttcttcgt cgggttgaag acgaagcggt cgccgatgcg cccgacgcgg
     1321 accgcgccga ggacctccgt gaacgggatc gggttcacgg cgagggccgc ggccgtgccg
     1381 ttgatggcga ggacgtcggg gtcgttctcg ccgtcggccg agaggacgag ggcgacgacc
     1441 tgcgtctcga aggcgtatcc ggccgggaag agcggccgga tcggccggtc gatcatccgg
     1501 caggtcagga tctccttctc ggtggggcgt ccctcgcgct tgaagaagcc tccggggatg
     1561 cgcccggcgg cgtactggta ttccttgtac tcgaccgtga gcgggacgaa atcgacgtcc
     1621 ttcgggtcgc gcgcgtagca cgcggtgacg aggaccatgg tgtcgccgag acggacgaca
     1681 gccgagccgt cggcctgctt ggccaccttt ccgttctcga tcgtgagggc acggcctccc
     1741 accagggcgg tgacggattc cttcatcgtt tccttctttc ggccggggag gccgcggcgc
     1801 ggccccgcgc cggcacggct cctacttgcg gagcccgagg cgctcgatca gggccttgta
     1861 gcgcgtcacg tcgcgcttct tcaggtaatc gagcaggcgg cgccgctggc cgaccatctg
     1921 gagcagtccg cggcgggagt ggtggtcctt cgcgtgcgtc ttgaagtgct cggtcagggc
     1981 cgtgatccgc tgcgagagga gcgccacctg cacctcgggg gagccggtgt cgccctcgtg
     2041 cctctcgaag gccttgatcg tttccgtctt gggagtgatg gtctgggcca cggttctcta
     2101 tccttctctt ctcaatctcg aacggcagag gataaggtcc gccgtcgatc gggtcaattt
     2161 cggggccaag tctctgtccg gtgagaagat gccgtctggc ccgttgtcgg tccgcgtgtc
     2221 tggaaaggga gccggagccg ggccgacagg ccccggttcc cccgggaaca cgattctatc
     2281 tgtccgcccg cgcggagggg gtccggggga accggcgtcg ggcctgccgg ccccggttcc
     2341 cccgggaaca cgattctatc tgtccgcccg cgcggagggg gtccggggga accggcgtcg
     2401 ggccgacagg ccccggttcc cccgggaaca tttcagtctt gtagcacgat ctt
//
LOCUS       DAOTEZ010000562         2446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_35369_length_2446_cov_6.498536, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000562 DAOTEZ010000000
VERSION     DAOTEZ010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35369_length_2446_cov_6.498536"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..707
                     /locus_tag="P5164_19405"
     CDS             147..707
                     /locus_tag="P5164_19405"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HRY46106.1"
                     /translation="METLTPPYLLLAAPVLSDPNFEKAVVLMGHHTEEGAVGWVVNRL
                     VEGGAKALLPDEVARTIHPDTPLRIGGPVATPGLIVVHREPVDGVESIAMAPGLVVSA
                     TPEVLPHVFASAPDASPVDGLLVFGYAGWGPGQLEHEMEDGSWLVLPYDPDFAFPADI
                     AGLWERSLARLGVTPGRVSSPPGGVN"
     gene            complement(853..>2446)
                     /locus_tag="P5164_19410"
     CDS             complement(853..>2446)
                     /locus_tag="P5164_19410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46107.1"
                     /translation="TVYLLVEPHEPMAEAARLTLENGVALHLVDRDDGSYPLRREALP
                     DPYALTKTGPAPFLSALLDAFPPSDDEADLLRERAMAVRLRRLADAGERVLWVGGVAH
                     ARGILRALDEPLAEPFGRVRREGVRLAALSAESSREVMSEIPFVAAAYESARSSGGAF
                     RFDAGTDTLRVVDRLLREAAERYRKSRDEEVPRTAFRVLRQFSRNLALTENVLTPGFY
                     ELVVAARGVVDDDYAWEVFDLGATWPWPDTTASLPVVTLKGEDLHLDGKPVRFRRRFP
                     GKERRLRDLPLRRRPKEPKPGDWRKLKFGDGICSYPPEDVAVERFGNRLRRRAIRLLT
                     EDSRRLAPFTTSLLDGVDVRETLRRHHEGRLWVFEEARVRGGAGSVVVVFDEEAEKYP
                     WKTTWLGEHGQESDMAFYATPLGEKLVGPGISECTYGAFLMTMPPGRLYDVWRDPDYR
                     GPFTAPEVLLLAALDYAREPRVVYAAPSPPRPLLKRFASRLGKRIVYLPLGSLASLSL
                     TKLRRFHVLANREARGWAKDFV"
BASE COUNT          332 a          839 c          958 g          317 t
ORIGIN      
        1 gacaaggacc gcgtccgcga ggccgaggcg aagtcgatcg cgaaggactt cgaggtcgcc
       61 ctctcgcact acacctacct cgaggagtag cccgggcggg atcgccgccg ctggcggggc
      121 gagtcgcggc ggcgtacgct tccggcgtgg agaccctgac cccgccctac ctgctgctcg
      181 ccgcgccggt cctctcggat ccgaacttcg agaaggccgt cgtcctgatg gggcaccaca
      241 ccgaggaggg ggcggtcggc tgggtcgtga accgcctcgt ggaggggggg gcgaaggcgc
      301 tcctccccga cgaggtcgcc cggacgatcc acccggacac acccctccgg atcggcggac
      361 cggtcgcgac gccggggctc atcgtcgtcc accgcgagcc ggtcgacgga gtcgagtcga
      421 tcgccatggc ccccgggctc gtcgtatcgg cgacacccga ggtccttccc cacgtcttcg
      481 cctccgcgcc cgacgcgtcc ccggtcgacg ggctgctcgt cttcggctac gccggctggg
      541 ggcccgggca gctggagcac gagatggaag atggctcctg gctcgtcctc ccctacgacc
      601 ccgacttcgc gtttcccgcc gacatcgccg gcctctggga gcggtcgctc gcccgcctcg
      661 gcgtcacgcc cgggcgcgtc tcgagcccgc ccggcggcgt gaactgaagt ggatcgggga
      721 gggtcgccac gcccctcccc gcgccgcttt cggcggaccc aggcgggcca ggacccaggg
      781 ggtctacctt ctataccctg ctcgggcgca gagggtgggg gataggcccg acgcgccgtt
      841 ccggacgcgc gcctagacga agtccttcgc ccagccgcgg gcctcgcgat tcgcgaggac
      901 gtggaagcgc cggagcttcg tcagcgagag ggaggcgagg gagcccagcg ggaggtagac
      961 gatcctcttc ccgaggcggg aggcgaagcg cttcaacaga gggcgcggcg ggctcggcgc
     1021 cgcgtagacg acgcgcggct cgcgggcgta gtcgagggcg gcgaggagga ggacctccgg
     1081 ggcggtgaac gggccgcggt agtcggggtc gcgccagacg tcgtagaggc ggccgggggg
     1141 catcgtcatc aggaacgcgc cgtaggtgca ctcggagatg ccggggccga cgagcttctc
     1201 gccgagcggc gtggcgtaga aggccatgtc gctctcctgg ccgtgctcgc cgagccaggt
     1261 cgtcttccac gggtacttct ccgcctcctc gtcgaagacg acgacgacgg agccggcgcc
     1321 gccgcggacc cgcgcctcct cgaagaccca gaggcggccc tcgtggtggc ggcggagcgt
     1381 ctcgcggacg tcgacgccgt cgaggaggct cgtcgtgaac ggggcgaggc ggcgcgagtc
     1441 ctcggtgagg agtcggatcg cgcggcggcg gaggcggttg ccgaagcgct cgacggcgac
     1501 gtcctcgggc gggtaggagc agatgccgtc gccgaacttc agcttcctcc agtcgcccgg
     1561 cttcggctcc ttcgggcggc gtcggagcgg caggtcgcgc aggcgcctct ccttccccgg
     1621 gaagcggcgg cggaagcgga ccggcttgcc gtcgaggtgg aggtcctcac ccttgagcgt
     1681 gacgacgggg aggctcgcgg tcgtatcggg ccagggccag gtggcgccaa ggtcgaagac
     1741 ctcccaggcg tagtcgtcgt cgacgacgcc gcgcgccgcg acgacgagct cgtagaagcc
     1801 cggcgtcagg acgttctccg tgagggcgag attccgggag aactgccgga ggacccggaa
     1861 ggccgtccgc ggcacttcct cgtcgcggct cttccggtag cgctccgccg cctcgcggag
     1921 gagccggtcg acgacgcgga gggtgtcggt gccggcgtcg aagcggaagg cgccgccaga
     1981 agagcgggcg gactcgtagg ccgccgcgac gaacgggatc tcgctcatca cctcgcggct
     2041 cgactcggcc gagagcgccg cgaggcggac cccttcgcgc cgcacgcggc cgaagggctc
     2101 ggcgagaggc tcgtcgagcg cgcggaggat gccgcgggcg tgcgcgacgc cgcccaccca
     2161 gaggacgcgc tcgcccgcgt cggcgaggcg gcggaggcgg acggccatcg cccgctcgcg
     2221 aaggaggtcg gcctcgtcgt cggacggcgg gaaggcgtcg aggagcgcgg agaggaacgg
     2281 cgccgggccg gtcttcgtga gagcgtacgg gtcggggagc gcctcgcggc ggagcgggta
     2341 gctgccgtcg tcccggtcga cgaggtggag cgcgacgccg ttctcgaggg tgaggcgcgc
     2401 ggcctcggcc atcggctcgt gcggctcgac gaggaggtag accgtc
//
LOCUS       DAOTEZ010000563         2444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_35404_length_2444_cov_5.860193, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000563 DAOTEZ010000000
VERSION     DAOTEZ010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35404_length_2444_cov_5.860193"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2444
                     /locus_tag="P5164_19415"
     CDS             <1..>2444
                     /locus_tag="P5164_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRY46108.1"
                     /translation="ALVPVAAYGLLSPMVAAGAMVFSSLFVVTNSLRLRRAPLEAFVP
                     REAAWRRGLALAPRVLAPAAALAALIVVPMLAMADGAEIRGALSGTMSPALMMVMAIA
                     NGVIAVSYASIPIFLVVFKVRRKDLPFSWVLVLFGLFILACGTTHFVHVLGLWWPVGW
                     WQATVDSICAAASLATAVVLWPLLPRILALPSPAQLRVVNRELEDEKVSLERTRDELR
                     RAYAEVERRVEERTAELAAANAALVAEVAVREAAEEELRRSRNMLAHVMDAIPQSVFW
                     KDRESVYLGCNRQMALRAGLRSPSEIVGRTDFDLPWTRSEAEAYRADDRDVVASRRPK
                     LHILETQRLPDGSTTWLDTSKVPLTNGGGEVYGVLGIFEDVTDRREAEARLHEYRKVI
                     ESSTDLICVLDGSRRFVLANGALLSFRGAPGEPVAGRGVGDVFGEEAVASLGPALDEC
                     FAGRERELELIVESTGRGRRDLSALLTPVLEGDAVTRVACVLRDVTEQRKLEAQLLQA
                     QKMEAIGQLAGGVAHDFNNILSVILGYCHLLGRGLSDDAKQAERLGQVVAAAERASQL
                     TRALLAFSRKQAMSPKALNLNDVVLKVQKLLLRIIGEDVRLRTVTNDATLAVFADGGQ
                     LEQVLINLATNARDAMPTGGLLTIETGLQVLDERFVALHGWGAPGRYAVLSVADTGCG
                     MDEETRKRIFEPFFTTKATGKGTGLGMSIVYGIVKQHRGFLSVYSEPGAGSVIRIYLP
                     VHEAGPAADEEPAETPAVRGGNETILVAEDEASVRRLLSTVLTEAGYDVVLAEDGQDA
                     VEKLSARRGDVHLVLL"
BASE COUNT          331 a          847 c          897 g          369 t
ORIGIN      
        1 cgcgctcgtc ccggtcgccg cctacggcct cctcagcccg atggtggccg ccggcgcgat
       61 ggtcttcagc tctctcttcg tcgtgacgaa cagcctgcgg ctccggcgtg cgccgctcga
      121 ggcgttcgtc ccgcgggagg ccgcctggcg gcgaggtctc gcgctcgcgc cgcgcgtcct
      181 cgcgcccgcg gcggcgctcg cggcgctgat cgtcgtcccg atgctcgcga tggcggacgg
      241 ggcggagatc cggggggccc tctccgggac gatgtcgccg gcgctgatga tggtcatggc
      301 gatcgccaac ggcgtgatcg ccgtctcgta cgcctcgatc ccgatcttcc tcgtcgtctt
      361 caaggtccgc cggaaggacc ttccgttctc gtgggtcctc gtcctcttcg ggctcttcat
      421 cctcgcgtgc gggacgacgc acttcgtcca cgtcctcggc ctctggtggc ccgtgggctg
      481 gtggcaggcg accgtggatt cgatctgcgc cgccgcgtcg ctcgcgaccg cggtcgtcct
      541 ctggccgctc ctcccgcgga tcctcgcgct tccgagcccc gcgcagctgc gcgtcgtgaa
      601 ccgggagctc gaggacgaga aggtctccct cgagaggacg cgggacgagc ttcgtcgggc
      661 gtacgccgag gtggagcgcc gcgtcgagga acggacggcc gagctggccg ccgcgaacgc
      721 ggctctcgtc gccgaggtgg ccgtccggga ggcggcggag gaggagctgc gccggagccg
      781 aaacatgctc gcgcacgtga tggacgcgat cccgcagtcg gtcttctgga aggacaggga
      841 gagcgtctac ctcggctgca accggcagat ggctctgcgg gccggcctgc ggtccccttc
      901 ggagatcgtc ggccggacgg acttcgacct cccctggacg cggagcgagg ccgaggccta
      961 ccgcgccgac gaccgcgacg tggtcgcctc ccggcggccg aagctccaca tcctcgagac
     1021 ccagcggctg ccggacgggt cgaccacgtg gctcgacacg tcgaaggtcc ctctcacgaa
     1081 cggaggcggc gaggtgtacg gggtcctcgg gatcttcgag gacgtgacgg accggcggga
     1141 ggcggaagcg cggctccacg aataccggaa ggtgatcgag agctcgacgg acctgatctg
     1201 cgtcctcgac ggctcccgcc ggttcgtcct cgccaacggc gccctcctct cgttccgcgg
     1261 cgctccgggc gagcccgtcg cgggacgggg cgtcggagac gtgttcggcg aggaggccgt
     1321 cgcgtctctc ggtccggcgc tggacgagtg cttcgccggg cgggagcgcg agctcgagct
     1381 gatcgtggag tctaccggga ggggccggcg cgatctctcg gccctcctga cgccggtcct
     1441 cgagggcgac gccgtgacgc gcgtggcctg cgtcctccgg gacgtcacgg agcagcgtaa
     1501 gctcgaggcg cagctcctgc aggcgcagaa gatggaggcg atcggccagc tcgccggggg
     1561 cgtcgcccac gacttcaaca acatcctctc cgtgatcctc ggctactgcc atctgctcgg
     1621 ccgcgggctc tcggacgacg cgaagcaggc cgagcgcctc ggccaggtcg tcgcggccgc
     1681 ggagagggcc tcccagctga cgcgggctct cctcgcgttc agccgcaagc aggcgatgtc
     1741 gccgaaggcc ctgaacctga acgacgtcgt cctcaaggtc cagaagctcc tcctccggat
     1801 catcggcgag gacgtgcggc tccgcacggt gacgaacgac gcgacgctcg ccgtcttcgc
     1861 cgacggcggc cagctcgagc aggtgctgat caacctcgcg acgaacgcga gggacgcgat
     1921 gccgacgggc gggctcctga cgatcgagac ggggctgcag gtcctcgacg agaggttcgt
     1981 ggcgctccac ggctggggcg ctccgggacg ctacgcggtc ctctccgtgg ccgacacggg
     2041 ctgcgggatg gacgaggaga cgcggaagag gatcttcgag ccgttcttca cgacgaaggc
     2101 gacggggaag gggaccggcc tcgggatgtc gatcgtctac gggatcgtga agcagcaccg
     2161 gggattcctg agcgtctaca gcgagcctgg cgccggaagc gtgatccgga tctatctccc
     2221 ggtccacgag gccggcccgg cggcggacga ggagcctgcg gagacgccgg ccgtccgcgg
     2281 cgggaacgag acgatcctgg tcgcggagga cgaggcgagc gtccgtcgcc ttctctcgac
     2341 ggtcctgacc gaggcaggct acgacgtcgt cctcgcggag gacggccagg acgcggtcga
     2401 gaagctttcg gcccgccgcg gcgacgtgca cctcgtcctc ctgg
//
LOCUS       DAOTEZ010000564         2439 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_35534_length_2439_cov_6.782718, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000564 DAOTEZ010000000
VERSION     DAOTEZ010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35534_length_2439_cov_6.782718"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1957)
                     /locus_tag="P5164_19420"
     CDS             complement(<1..1957)
                     /locus_tag="P5164_19420"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRY46109.1"
                     /translation="MRRWASLAATLALLGSPARALDPSGPPQEFAKTIFREELPQASV
                     LALLQSSDGYLWLGTYEGLVRFDGVEFQVWDRQTYPAMRTNVAQCLAEDASGGIWFGT
                     SRGGVGRLKDGVVEVFGHGSGLPSDDVTSVFRERSGAVWIGTDRGVARWSNGRFEAFG
                     ADHGFGPGAVLALAESSDGTVWAGGRGLRVFREGRWSDTEVPPGVELPITALAADRDG
                     TLWIGSISGVTRTDRAGSRTFRIGDGLAGGWVRCLLRDRSGTTWAGTEGRGLFRLRGE
                     DFEAFSPVVEEKATDFVISLLEDREGSLWLGTRGGLIRMSEGVFTSLGAEQGLVGELA
                     RTVFQDSSGRIWVGTDGGGLHLVEGGRCTPMNARLRIGSRRVRTVGQEPGGALWVGTS
                     GEGVFRVGDGRTERIGRSEGLADEDVRVIVRAHDGTMWVGTRTGLAAFRDGRALPASE
                     LSRRAGGTNALLEARDGSLWVGTAGRGVLVARGGAIAAITAAAGLAGDTVFALHEDRD
                     GTVWVGTADGLSRVTGGKPTTLRAPEALFRDQVFSILEDDAGGLWMSNNRGVFRFPAG
                     SLAAAGETSQGPLGRLAFGVADGMPSRQCNGTTQPAALRARDGRLWYPTANGVAIADP
                     ELLRPSAPPPPVVLLRALVDGAAVPISG"
     gene            2178..>2439
                     /locus_tag="P5164_19425"
     CDS             2178..>2439
                     /locus_tag="P5164_19425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY46110.1"
                     /translation="MSSIPPATYDVVVIGSGPGGYVAAIRAAQLGLKTAIVEKDAKTP
                     GIGLGGTCLHRGCIPTKAMLKSATLFDEVKHASDFGVRTTGVE"
BASE COUNT          352 a          981 c          794 g          312 t
ORIGIN      
        1 cccccgagat cggcaccgcg gccccgtcga cgagcgcccg cagaaggacg accggaggcg
       61 gcggcgcgct cgggcgaagc agctccgggt cggcgatcgc gaccccgttc gcagtcgggt
      121 accaaagcct tccgtctcgc gcgcggaggg ccgcgggctg cgtcgtcccg ttgcactgac
      181 ggctcggcat gccgtcggcg acgccgaagg cgagccggcc gaggggcccc tgcgacgtct
      241 cgccggcggc cgcgaggctt cctgccggga acctgaagac gcccctgttg ttgctcatcc
      301 agagcccgcc cgcatcgtcc tcgaggatcg agaagacctg atcccggaag agcgcctccg
      361 gcgcccgcag cgtcgtgggc tttccccccg tcacgcggct cagcccgtcc gccgttccga
      421 cccagaccgt cccgtcccgg tcctcgtgaa gcgcgaagac agtgtctccc gcgagcccgg
      481 ccgcagccgt gatcgctgcg atggctcctc ctcgcgcgac gaggacgcct cgcccggccg
      541 ttccgaccca gaggctcccg tcgcgcgcct ccagaagcgc attcgtgccg ccggctctcc
      601 gcgagagctc cgaggccggc agcgcccggc cgtcgcggaa cgccgccagg ccggtccgtg
      661 tccccaccca catcgtcccg tcgtgcgccc ggacgatgac gcgcacgtcc tcgtcggcga
      721 gcccctcgct ccggccgatt cgctccgtcc gaccatcccc gacccggaag accccctccc
      781 cggaggtccc gacccagagc gcgccgccgg gctcctggcc gaccgtccgg acgcggcggc
      841 tcccgattcg gagccgggcg ttcatcggcg tgcatcggcc accctcgacg aggtgcagcc
      901 cgccgccgtc cgtgccgacc cagatgcggc cggaggagtc ctggaacacg gtgcgcgcga
      961 gctcgcccac gagcccctgc tcggccccga gcgacgtgaa gaccccttcg ctcatccgga
     1021 tgagcccgcc tcgcgtcccg agccagaggc tcccttctcg gtcctcgagg agcgagatga
     1081 cgaagtccgt cgccttctcc tccacgacgg gcgagaaggc ctcgaagtcc tccccgcgaa
     1141 gccggaagag cccgcgcccc tccgttccgg cccaggtcgt tccggagcgg tcccggagga
     1201 ggcagcggac ccagccaccc gcgaggccgt cgccgatccg aaacgtccgg ctcccggcgc
     1261 gatccgtccg cgtcacgccg gagatcgagc cgatccagag ggtcccgtcc cgatcggcgg
     1321 cgagcgcggt gatcgggagc tcgactcccg ggggaacttc cgtgtcgctc catcggccct
     1381 cccggaacac ccgcagcccg cgcccgccgg cccagacggt cccgtcggag gactccgcca
     1441 gggcgagcac ggctccggga ccgaacccgt gatcggcgcc gaacgcttcg aagcgtccgt
     1501 tcgaccagcg ggccacgccc cgatccgtgc cgatccagac agccccggac cgttctcgga
     1561 agacgctcgt cacgtcgtcg gacggcaggc ccgacccgtg tccgaagacc tcgaccacgc
     1621 cgtccttcag gcggcccacg cctccccggc tcgttccgaa ccagattcct cccgacgcgt
     1681 cttccgcgag acactgggcg acgttcgtcc gcatcgcagg gtacgtctgg cggtcccaga
     1741 cctggaactc cactccgtcg aaccggacga gcccctcgta ggtccccagc cagagatagc
     1801 cgtcggaaga ctggagcagg gcgaggacgg aggcctgcgg aagctcctcc cggaagatcg
     1861 tcttcgcgaa ctcttgcggc ggcccggacg gatcgagcgc ccgggcgggc gagccgagca
     1921 gagccagcgt cgccgccagg ctcgcccacc tgcgcatccc ccgaggtcgc accgatccgc
     1981 gtcggaccgt ccacgcgcgc atccttcgag ttcctgctct ttccgacgac cgcctgcagc
     2041 cgacccggcg attatcgacc cggcggccgg gctccggcgc gccgcgaggc cctccggagg
     2101 cgccccctat aatcgccgct cacgaaagcg cccgggaccc gtgctcgtac cggcgaggcg
     2161 cccaggagac acgagagatg agcagcattc cccccgcgac gtacgacgtc gtcgtcatcg
     2221 gttctggccc cggcggctac gtcgccgcca tccgcgccgc gcagctcggc ctgaagaccg
     2281 cgatcgtcga gaaggacgcg aagacgcccg gcatcggcct cggcggcacg tgcctccacc
     2341 gcggctgcat cccgacgaag gcgatgctca agtcggcgac cctgttcgac gaggtcaagc
     2401 acgcctccga cttcggcgtc cggacgacgg gcgtcgagc
//
LOCUS       DAOTEZ010000565         2438 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_35557_length_2438_cov_7.347881, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000565 DAOTEZ010000000
VERSION     DAOTEZ010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35557_length_2438_cov_7.347881"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="P5164_19430"
     CDS             complement(<1..197)
                     /locus_tag="P5164_19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRY46111.1"
                     /translation="MTRREEDLGRFEEALGFRFGDRSLLERALTHSSYANEASRAGAV
                     RDNETLEFLGDSVLGFLVAEML"
     gene            complement(257..988)
                     /locus_tag="P5164_19435"
     CDS             complement(257..988)
                     /locus_tag="P5164_19435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46112.1"
                     /translation="MSFRLLALVPALALALPAFAQSKLLVVLRNGEVRTFELAEVERI
                     EFAGTSLTGGVDPARPVFRDPVGTWDWVDGQTLVLRADSQFALHRSDGTLISQGRWEL
                     LESATQRYRLVHARGGYIDTVALSADGWTLSGASNTGYRLEGRRRSDAATGWGGASEA
                     QLAGTWDWADGRVLVLRTDRTFLAYSGSRQTDEGRWQVLDAAARRIQLTHRLAGTTDT
                     LVLSADGRMLEGTNNRGARVRGQKR"
     gene            complement(1057..>2438)
                     /locus_tag="P5164_19440"
     CDS             complement(1057..>2438)
                     /locus_tag="P5164_19440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46113.1"
                     /translation="GPRRAVRVRVPPPADAREATGPVALSTRAILAFWLPLAATWLMM
                     SVEGPYLSAIVARLVDPVPNLAAFGVAFTLAWLAESPIIMLLTATTALAKDRASFLAF
                     RRFATRLNGAVTAALLVLVLPPVFRFVAERLLALPPEVARLAHLATALLVPWPAAIGY
                     RRFYQGVLVRNRMTRRVAYGTVVRLATMSATAAALATATRLPGALIAAAALSVGVVAE
                     AAASRFMARHLVAGLLSENGDASPPPQRELARFYFPLALTSMISMTTAPLLTFFMSHG
                     PNPVPSLAAWPVVSALVFLFRSGGVGFQETSVALSGEAGENVPAVRRTGALLGAGASL
                     LLAAVAFSPLLGLWYRVVVGLGPELIPVAVVPTRILVLLPALEYALSFLRSRWVLARQ
                     TRPVTLATALEAVGLLSGMVLLVVVARLPGTIAAALALLLGRLVSCAFLVWLGRTARA
                     RRAFVSARP"
BASE COUNT          385 a          899 c          889 g          265 t
ORIGIN      
        1 agcatctcgg cgacgaggaa cccgaggacg ctgtcgccca ggaactcgag cgtctcgttg
       61 tcgcgcacgg cgccggcgcg cgaagcctcg ttcgcgtacg aggagtgcgt cagcgcgcgc
      121 tcgaggaggg accggtcccc gaaccggaac ccgagcgcct cctcgaaccg cccgagatcc
      181 tcttctcgtc tcgtcatctc ggctctccgg ccgccgggcc gcgggcgtcg ccaccctcga
      241 cccggcgcgt cgcgttctac ctcttctgcc cccggacgcg ggcgccgcgg ttgttcgtcc
      301 cttcgagcat ccgcccgtcc gccgacagga ccagcgtgtc ggtggtcccg gccagccggt
      361 gcgtcagctg gatgcggcgg gcggcggcgt cgagcacctg ccagcggccc tcgtccgtct
      421 ggcggctccc cgagtaggcc agaaacgtcc ggtcggtccg cagcaccagg acgcggccgt
      481 ccgcccagtc ccacgtcccg gcgagctgcg cctcggaggc gccgccccag cccgtcgccg
      541 cgtcggagcg acgccgtccc tcgaggcggt agcccgtgtt cgaagccccg gagagcgtcc
      601 agccgtcggc ggaaagcgcg acggtgtcga tgtagccgcc gcgcgcgtgg acgaggcggt
      661 agcgctgcgt ggcgctctcg aggagctccc agcgcccctg gctgatcagc gtcccgtcgc
      721 tcctgtggag cgcgaactgg gagtcggcgc gaaggacgag cgtctgcccg tcgacccagt
      781 cccacgttcc gaccggatcg cgaaagaccg gacgggccgg atcgacgcct ccggtcagcg
      841 acgtgccggc gaactcgatc cgctccacct cggccagctc gaaagttcga acctctccgt
      901 tcctgaggac gacgaggagc ttcgactgag cgaacgcagg cagagcgagg gcgagcgccg
      961 ggaccagggc gaggagacgg aaggacattg cgacctcctt gaccggccgg aataccacgc
     1021 cggacgtttc cgcgccaccg tctcgggccg ggcgtctcag gggcgggcgg acacgaaggc
     1081 cctccgcgcc cgcgccgtcc ggcccagcca gacgaggaac gcgcaggaca cgaggcgtcc
     1141 gagcaggagg gcgagcgccg ccgcgatcgt ccccgggagc ctcgccacga cgacgaggag
     1201 caccatgccc gagaggagcc cgacggcctc gagggcggtg gccagagtca cggggcgcgt
     1261 ctgccgcgcg aggacccacc gcgagcggag gaacgacagc gcgtactcga gcgccgggag
     1321 gaggacgagg atccgcgtcg ggacgacggc gaccgggatc agctcgggcc ccagcccgac
     1381 gacgacgcgg taccagagcc cgaggagcgg cgagaacgcg accgcggcga ggaggagcga
     1441 cgctcccgct ccgaggagcg cccccgtccg gcgaaccgcc gggacgttct ctcccgcctc
     1501 cccgctcaat gccacgctcg tctcctggaa cccgacgccc ccgctccgga agaggaagac
     1561 gagagcgctc acgaccggcc acgccgcgag cgacggcacc gggttcgggc cgtggctcat
     1621 gaagaacgtc agtagcggtg ccgtcgtcat cgagatcatc gacgtgaggg cgagcgggaa
     1681 gtagaagcgc gcgagctctc gctgcggcgg aggagaggcg tccccgttct ccgacaggag
     1741 ccccgccacg agatgacgcg ccatgaaccg gctcgccgcc gcctcggcga cgaccccgac
     1801 gctcagcgcc gccgccgcga tcagcgctcc ggggagccgg gtcgccgtcg cgagcgcggc
     1861 cgccgtcgcg gacatcgtcg cgagccggac gaccgtgccg tacgcgaccc gccgcgtcat
     1921 ccggttccgc acgaggaccc cctggtagaa gcggcggtac ccgatcgcgg cgggccacgg
     1981 cacgaggagc gccgtcgcca ggtgcgcgag gcgtgcgacc tcgggcggca gcgcgaggag
     2041 cctctccgcg acgaagcgga agaccggcgg gaggacgagg acgaggaggg ccgccgtcac
     2101 ggcgccgttg aggcgcgtcg cgaagcgccg gaacgcgagg aacgaagccc ggtccttcgc
     2161 cagcgccgtc gtcgccgtca ggagcatgat gatcggcgac tccgcgagcc acgcgagcgt
     2221 gaacgcgacg ccgaacgcgg cgaggttcgg caccgggtcg accaggcgcg cgacgatcgc
     2281 ggagaggtac ggcccctcca ccgacatcat cagccacgtc gccgccagcg gcagccagaa
     2341 cgcgaggatc gcccgcgtcg agagggcgac cggcccggtc gcctctcgag cgtctgccgg
     2401 aggagggact cgaaccctca cggcccttcg gggcccgg
//
LOCUS       DAOTEZ010000566         2426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_35869_length_2426_cov_7.913960, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000566 DAOTEZ010000000
VERSION     DAOTEZ010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35869_length_2426_cov_7.913960"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1850)
                     /locus_tag="P5164_19445"
     CDS             complement(<1..1850)
                     /locus_tag="P5164_19445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HRY46114.1"
                     /translation="MRLRVLAPAAALAFAALGARAAEPPAPLAAFVRESYTKYEHLVP
                     MRDGTRLFTAVYVPKDAGPAKRYPILMNRTPYSVAPYGVDAYPASLGPSEAAAREKFV
                     FVYQDVRGRMMSEGTFVDVRPVLPTKGPKDVDETTDAFDTVDWLVKNVPHNNGKVGTW
                     GISYPGFYAALAAIDAHPALVAVSPQAPVADWFAGDDFHHNGALFLAAAFNFYVRFGQ
                     PREGPTTKWAPRFDHGTMDGYRFFLEAGSAVGLSKHMKGVAFWDDLMAHETYDAYWQA
                     RNTRPHLKGIRPAVLTVGGWYDAEDAFGALETYRAVERQSPGASNRLVVGPWWHGGWA
                     RSDGESHGRVTFGQKTSVRYREEVELPFFLHHLKGAPDPKLPEATVFETGRNVWRSFD
                     AWPPREAKATAFRLGAGGVLSADAPPSGSAVAAEWVSDPAKPVPYVESVDIGMDAEYM
                     TADQRFAARRPDVAVWQTPPLEADLTVAGPIEVSLSVSTSGTDADWVVKLIDVWPDDV
                     PVEAWEWTPRNTWDEKPTRSKLGGNQQLVRGEPFRGRFRKSLSSPEPFTPEKVEAVAF
                     RMPDVLHTFRRGHRVMVQVQSSWFPLVDRNPQTFVPIRTAKESDFRPATMR"
     gene            1936..>2426
                     /locus_tag="P5164_19450"
     CDS             1936..>2426
                     /locus_tag="P5164_19450"
                     /inference="COORDINATES: protein motif:HMM:NF013560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HRY46115.1"
                     /translation="MQKTLPTLLLLLLVPMTGAAQAPTPADAKAFVEKAEEQLLKASI
                     AAERANWVASNFITEDTERIAADGNQRRMELAAGLAKEATRFDGLALDAETARKLKLL
                     KLSLVLAAPSDPAKSEALARIAAGMEAAYGRGKWTPPGGEPLSLEEISQRLATSRDPK
                     ELAA"
BASE COUNT          354 a          848 c          885 g          339 t
ORIGIN      
        1 cgcatcgtgg ccgggcggaa gtcgctctcc ttcgcggtgc gaatcgggac gaacgtctgc
       61 gggtttcggt cgacgagcgg gaaccagctg ctctgcacct gcaccatcac gcggtgcccg
      121 cgccggaacg tgtgaaggac gtcgggcatc cggaaggcga ccgcctccac cttctccggc
      181 gtgaacggct cgggcgagga gaggctcttc cggaaccgtc cgcggaacgg ctcgccgcgg
      241 acgagctgct ggttgccgcc gagcttcgag cgggtcggct tctcgtccca ggtgttgcgc
      301 ggcgtccact cccacgcctc gacgggaacg tcgtcgggcc agacgtcgat gagcttcacg
      361 acccagtcgg cgtcggtccc gctcgtcgag acggagaggc tcacctcgat cggtccggcg
      421 acggtgaggt ccgcctcgag cggcggcgtc tgccagacgg cgacgtcggg acggcgcgcg
      481 gcgaagcgct ggtcggccgt catgtattcg gcgtccatcc cgatatcgac cgactcgacg
      541 tacggcaccg gcttcgccgg gtcgctcacc cactcggcgg cgaccgcgct cccggaaggc
      601 ggggcgtcgg cggagaggac gccgcccgcg ccgaggcgga aggcggtcgc cttcgcttcg
      661 cgcggcggcc aggcgtcaaa ggagcgccag acgttccttc cagtctcgaa gacggtcgcc
      721 tcggggagct tcgggtcggg ggcccccttg aggtgatgga ggaagaaggg gagctcgacc
      781 tcctcgcggt agcgaacgga ggtcttctgc ccgaaggtga cccgcccgtg ggattcgccg
      841 tcgcttcgcg cccagccgcc gtgccaccag gggccgacga cgagccggtt gctcgcgccg
      901 gggctctgcc tctcgacggc ccggtaggtc tcgagcgcgc cgaaggcgtc ctccgcgtcg
      961 taccagcctc cgaccgtcag gacggccggc cggatcccct tcaggtgcgg tcgggtgttc
     1021 cgggcctgcc agtaggcgtc gtacgtctcg tgggccatca ggtcgtccca gaaggcgacc
     1081 cccttcatgt gcttcgagag gccgacggcc gagcccgcct cgaggaagaa gcgatagccg
     1141 tccatcgtcc cgtggtcgaa gcgcggcgcc cacttcgtcg tcggtccctc gcggggctgg
     1201 ccgaagcgga cgtagaagtt gaacgccgcc gcgaggaaga gcgcgccgtt gtggtggaag
     1261 tcgtcgcccg cgaaccagtc ggcgaccggg gcctgcggcg agacggcgac gagcgccggg
     1321 tgcgcgtcga tcgcggccag ggccgcgtag aagccggggt aggagatccc ccacgtcccg
     1381 accttcccgt tgttgtgcgg gacgttcttc acgagccagt cgacggtgtc gaatgcgtcc
     1441 gtcgtctcgt cgacgtcctt cggtcccttc gtcgggagga cgggccgcac gtcgacgaac
     1501 gtcccttccg acatcatccg gccccgaacg tcctggtaga cgaagacgaa cttctcgcgc
     1561 gccgcggcct ccgacggccc gagggaggcc ggatacgcgt cgacgccgta gggggcgacg
     1621 gagtacggcg tccggttcat caggatcggg taacgcttcg cggggcccgc gtccttcggg
     1681 acgtagaccg ccgtgaagag gcgcgtcccg tcgcgcatcg gaacgaggtg ctcgtacttc
     1741 gtgtagctct cgcggacgaa cgcggcgagg ggcgccgggg gctcggcggc ccgagcgccg
     1801 agagcggcga aggcgagggc tgcggccggg gcgaggacgc gaaggcgcat gacgggacct
     1861 ccctgggggg cggtggggcg aacgttagca gcaggcggac cgcgtgggat gatcctcggc
     1921 cggagcgatg agcgcatgca gaagaccctc cccaccctcc tccttctcct cctcgtcccg
     1981 atgacgggcg ccgcccaggc gccgaccccc gccgacgcga aggccttcgt cgagaaggcc
     2041 gaagagcagc tcctgaaggc ctcgatcgcc gccgagcgcg cgaactgggt cgcctcgaac
     2101 ttcatcaccg aggacaccga gcggatcgcc gcggacggga accagcgccg gatggagctc
     2161 gcggccggcc tcgcgaagga ggcgacccgc ttcgacggcc tcgccctcga cgccgagacg
     2221 gcccggaagc tgaagctcct gaagctctcc ctcgtcctgg ccgcgccgtc cgatcccgca
     2281 aagagcgagg cgctcgcccg gatcgccgcc gggatggaag cggcctacgg ccgcggcaag
     2341 tggacgccgc ccggcggcga gccgctctcc ctcgaggaga tctcccagcg cctcgcgacg
     2401 agccgcgacc cgaaggagct cgccgc
//
LOCUS       DAOTEZ010000567         2425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_35895_length_2425_cov_5.237131, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000567 DAOTEZ010000000
VERSION     DAOTEZ010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35895_length_2425_cov_5.237131"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /gene="miaE"
                     /locus_tag="P5164_19455"
     CDS             complement(<1..582)
                     /gene="miaE"
                     /locus_tag="P5164_19455"
                     /inference="COORDINATES: protein motif:HMM:NF017939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 -
                     tRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl
                     adenosine)-hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase
                     MiaE"
                     /protein_id="HRY46116.1"
                     /translation="MITLRTKTPDAWAERAAASLPDLVADHAACELQAAVFALALVGR
                     YPGDPLLVDRLSALAAEELRHFRKAMREARRLGAKVGTRRANAYVSTLRAACRAGREP
                     EQGLDLLLCGALVEARSHERFLALVPRLAAEPRLAKLYVELAAAEERHGPAYVELAER
                     HAGSEATAARLEELLDVEARAIAGAPASPVAVHS"
     gene            complement(579..1550)
                     /locus_tag="P5164_19460"
     CDS             complement(579..1550)
                     /locus_tag="P5164_19460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46117.1"
                     /translation="MKGEALPVLWARGLLSAARTWKALLVALALNAGLALVVTAPLAS
                     RLHDLLDDRPTAAPLAKGRDLVLEAHLFRAEPGLLGDPARLDALGRGSLPLAELPSLS
                     GPLAGLLAAGLLHSALSGLLAGGFVGRFASERDRGSLSAFFSDVVRLGLPSLLLGLLG
                     LGGILGAFYVFVELPRAATLSPDLRWDWERTGLSLLRLGAFLLAAGAIRAVVAQSRAA
                     LGLSRSVNPLAALGAGLGFVLGRPVKALLLEVAFGATALLPLALWLLLAPSFRGGDLR
                     LLAALVAGQQVVVLLRIASRAAHLGAASAWMRGAREASRPAPAKIES"
     gene            complement(1547..>2425)
                     /locus_tag="P5164_19465"
     CDS             complement(1547..>2425)
                     /locus_tag="P5164_19465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46118.1"
                     /translation="EAHLDEGLATWAAARAVTRLQEPPSPVLEAFGVPFALRLSRPLP
                     EGEAQDALDQAVFSRSDPTLRESWRALDDAAVRTNAYSRTALLLESVARTTGEQPLTA
                     AVAEYARRFAFRHPTTRDFLDVVGEVASVEARSLLERGWASAGTADFAVTKADTARMR
                     PPAGFLGEGPRRAWAAPVPGGGAWESTVVVQRLGELPWPVEVELRFEGGHVVKRTWDG
                     HGEWIRYRATGPRLLSAVVDPGRACLLDVNRLNDGLATEPDPTAARAWGHRLRFLAQN
                     VLELFALVAALPGAVR"
BASE COUNT          341 a          866 c          992 g          226 t
ORIGIN      
        1 ggagtggacg gcgaccggcg aggccggcgc tcccgcgatc gcgcgcgcct cgacgtcgag
       61 gagctcctcg agccgggccg ccgtagcctc gctccccgca tgccgctcgg cgagctcgac
      121 gtacgccggc ccgtgccgct cttcggcggc ggcgagctcg acgtagagct tcgcgaggcg
      181 cggctcggcg gcgagccgcg gcacgagcgc gaggaacctc tcgtggctcc gcgcctcgac
      241 gagggccccg cagaggagga ggtcgagccc ctgctcgggc tcgcgtccgg cgcggcaagc
      301 ggcccgcagc gtcgagacgt aggcgttcgc gcggcgcgtc ccgactttcg cgccgagccg
      361 ccgcgcctcc cgcatcgcct tccggaagtg gcgcagctcc tccgcggcga gggccgagag
      421 gcggtcgacg aggagcggat cgccggggta gcgcccgacg agggcgaggg cgaagacggc
      481 ggcctgcagc tcgcacgccg cgtgatcggc gacgaggtcg gggaggctcg ccgcggcccg
      541 ctcggcccag gcgtcggggg tcttcgtccg gagcgtgatc acgactcgat cttcgcaggc
      601 gcggggcgcg aggcctcgcg cgcgccgcgc atccaggccg aggcggcccc gaggtgagcg
      661 gcgcgcgacg cgatccggag gaggacgacg acctgctgcc cggcgacgag cgccgcgagg
      721 aggcggaggt cgccgccgcg gaaggacggg gcgaggagga gccagagcgc gagcggaagg
      781 agagccgtcg cgccgaaggc gacctcgagg aggagcgcct tgacggggcg gcccaggacg
      841 aacccgagcc ccgcgccgag cgcggcgagc gggttcacgg agcgcgagag gccgagcgcg
      901 gcgcgcgact gggcgacgac cgcccggatc gcgccggccg cgaggaggaa ggcgccgagg
      961 cggaggagcg agagcccggt cctttcccag tcccaccgga ggtcgggcga gagcgtcgcg
     1021 gcgcgcggga gctcgacgaa gacgtagaac gcgccgagga tcccgccgag gcccaggagg
     1081 ccgaggagaa gcgacgggag gccgagcctc acgacgtccg agaagaacgc ggagaggctc
     1141 ccgcggtccc tctccgaagc gaagcggccg acgaagccgc ccgcgaggag gcccgagagc
     1201 gcggagtgca gcaggcccgc ggcgaggagg cccgcgaggg gtcccgagag gctcggcagc
     1261 tccgcgaggg gcagcgagcc ccgcccgagc gcgtcgaggc gagccgggtc tccgaggagg
     1321 cccggctcgg cgcggaagag atgcgcttcg aggacgaggt cccggccttt cgcgaggggc
     1381 gcggccgtcg gccggtcgtc gaggaggtcg tggaggcgcg aggcgagcgg cgcggtcacg
     1441 acgagggcga gcccggcgtt cagcgcgagg gccacgagga gcgccttcca ggtccgcgcg
     1501 gccgagagga gtcctctcgc ccagagtacg gggagcgcct cgcccttcac cgtacggctc
     1561 ccggaagcgc cgcgacgaga gcgaagagct cgaggacgtt ctgtgccagg aagcggagcc
     1621 ggtgccccca ggcgcgcgcg gccgtcgggt cgggctccgt cgcgaggccg tcgttcaggc
     1681 ggttcacgtc gaggaggcag gcacggcccg ggtcgacgac ggccgagagg agcctcggcc
     1741 ccgtggcgcg atagcggatc cactcgccat gcccgtccca cgtccgcttc acgacgtggc
     1801 ccccctcgaa tcggagctcc acctccacgg gccacggcag ctcgccgagg cgctggacga
     1861 cgaccgtcga ctcccacgcg cccccgcccg ggaccggcgc cgcccacgcc cggcggggcc
     1921 cttcgccgag gaaaccggcg ggagggcgca tccgggcggt gtcggccttc gtgacggcga
     1981 agtcggcggt gccggccgag gcccagcccc gttcgaggag gctccgcgcc tcgacgctcg
     2041 ccacctcgcc gacgacgtcg aggaagtccc gcgtcgtcgg gtggcggaac gcgaagcgcc
     2101 gggcgtactc ggccacggcg gccgtcaggg gctgctcgcc cgtcgtccgg gcgaccgact
     2161 cgaggaggag cgccgtccgc gagtaggcgt tcgtccggac cgcggcgtcg tcgagggcgc
     2221 gccacgactc gcgcagcgtc gggtcggagc gggagaagac ggcctgatcg agggcgtcct
     2281 gcgcctcccc ctcggggagc ggccgcgaga gccggagcgc gaacggcacc ccgaacgcct
     2341 cgagaaccgg gctcggcggc tcctggagcc gcgtgacggc gcgggcggcg gcccaggtgg
     2401 ccagcccctc gtcgaggtgc gcctc
//
LOCUS       DAOTEZ010000568         2420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_36025_length_2420_cov_4.322199, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000568 DAOTEZ010000000
VERSION     DAOTEZ010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36025_length_2420_cov_4.322199"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="P5164_19470"
     CDS             <1..363
                     /locus_tag="P5164_19470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HRY46119.1"
                     /translation="DSGFKVVLSARVPDVVREHVALDIDAFLAEHGLDRSAIRHWIAH
                     TGGPRVLQAFEGALSLPETALARSWTSLREIGNLSSASVLFVLGDLLASGEPAAGDRG
                     LLLAMGPGFCSELVLLEW"
     gene            377..955
                     /locus_tag="P5164_19475"
     CDS             377..955
                     /locus_tag="P5164_19475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRY46120.1"
                     /translation="MTVPDSAALYLVLLALLGCERGAELVVSRRNARRAFAAGGVETG
                     RRAYAIMVAVHALFPLACAAEVLLLPRSFPGAAGFAALAVALGAQALRWWAVATLGER
                     WSTRVIVVPGAAPVTGGPYRFLRHPNYLAVVLEMAAVPLIHGAWLTALVFSALNAALL
                     AHRIPAEERALGRAYAEVFSERRRLVPGGPRG"
     gene            948..1244
                     /locus_tag="P5164_19480"
     CDS             948..1244
                     /locus_tag="P5164_19480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRY46121.1"
                     /translation="MAELREEVLAEIRRTLAADLDFRGPVELHHGLATDLQVDSLGAL
                     VLAVALEDRFRVKLTGIEAASVTSVEELVGVVERAVREAGGGGASRDADGGEGP"
     gene            1241..>2420
                     /locus_tag="P5164_19485"
     CDS             1241..>2420
                     /locus_tag="P5164_19485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRY46122.1"
                     /translation="MNGPPLPRPKHGTVNETLAASATSGAGLTFVDLAERETFHSYAD
                     VHARARRTAGALRARGVAAGDRVALVFPTSPAFVDCFFGVLLAGAVPVPLYPPVRLGR
                     LAEYLAATSRMIAAAGARLVLADAAARRLLGKAIEASRPALGCLTPEELGEGGELEAA
                     VGPDELGLIQFSSGSTAEPRPVALSHASLLAQLAALRALLPAEGAVPQKGVSWLPLYH
                     DMGLIGCLLQAVYYPGPLVLIPPELFLAKPALWLRAIARHRATISVAPSFAYALCSRK
                     VRDEELSGADLSSWRHALCGAEPVSPESLDDFARRFEPFGFDPRSLRPVYGLAEAALA
                     VTFTPAGRRVRSLRVDPARLAATGDVVDGTRAIASVGTPVPGTDVEVRDPDGLALEER
                     R"
BASE COUNT          233 a          957 c          863 g          367 t
ORIGIN      
        1 gactcgggct tcaaggtggt cctctcggcc cgggtgcccg acgtcgtgcg cgagcacgtg
       61 gccctcgaca tcgacgcgtt cctcgccgag cacggcctcg accgctcggc gattcgccac
      121 tggatcgcgc acaccggcgg cccgcgggtg ctccaggcgt tcgagggcgc gctctctctg
      181 cccgagacgg ccctcgcccg ttcctggacg tcgctccggg agatcgggaa cctctcctcc
      241 gcgtccgtcc tcttcgtcct cggagatctc ctcgcgtcgg gcgagcccgc ggccggggat
      301 cgcggactcc tcctcgcgat gggccccggc ttctgctcgg agctggtcct cctcgagtgg
      361 tagaggttcg gtcgacgtga cggtccccga ctccgctgcc ctctacctgg tcctgctcgc
      421 cctcctcggc tgcgagaggg gcgcggagct cgtcgtctcg cgccggaacg cccgccgggc
      481 gttcgcggcc ggcggcgtcg agacggggcg ccgggcctac gcgatcatgg tcgcggtcca
      541 cgcgctcttc ccgctcgcgt gcgcggccga ggtcctcctc ctgccgcggt ccttcccggg
      601 cgccgcgggc ttcgccgcgc tggcggtggc cctcggggcc caggcgctgc gctggtgggc
      661 cgtcgcgacg ctcggagagc gctggagcac gcgggtcatc gtcgtcccgg gcgcggcgcc
      721 ggtgactggc ggcccctatc gcttcctccg gcatccgaac tacctcgcgg tcgtcctcga
      781 gatggccgcc gtccccctga tccacggcgc ctggctcacc gcgctcgtct tctcggcgct
      841 gaacgcggcg ctcctcgccc accgcattcc cgcggaggag cgggccctcg gaagggcgta
      901 cgccgaggtc ttctccgagc gaagacggct cgtgccggga ggtccgcgtg gctgagcttc
      961 gcgaggaggt cctcgccgag atccgccgga ccctcgcggc cgacctcgat ttccgcggtc
     1021 cggtcgagct tcaccacgga ctcgcgaccg acctccaggt cgactctctc ggcgccctcg
     1081 tcctggccgt ggcgctcgag gaccgcttcc gggtgaagct cacgggcatc gaggccgcct
     1141 cggtcacgtc cgtcgaggag ctcgtcggcg tcgtcgagcg cgccgtccgc gaggcggggg
     1201 gcggaggcgc gtcgcgggac gccgacggcg gggaaggccc gtgaacgggc cgccgctccc
     1261 gcggccgaag cacggcaccg tcaacgagac cctcgccgcg agcgcgacga gcggagcggg
     1321 tctcacgttc gtcgacctcg ccgagcgcga gacgttccat tcgtacgcgg acgtccacgc
     1381 gcgcgcgcgc cggacggccg gggcgctccg ggcccgaggg gtcgcggccg gcgaccgcgt
     1441 cgctctcgtc ttcccgacct cgcccgcgtt cgtcgattgc ttcttcggcg tcctcctcgc
     1501 cggcgccgtg cccgtcccgc tctacccgcc ggtccgcctc gggcggctcg ccgagtacct
     1561 cgccgcgacg tcgaggatga tcgccgcggc cggcgcgcgc ctcgtcctcg ccgacgcggc
     1621 ggcccgcagg ctcctcggga aggcgatcga ggcctcccgc ccggcgctcg ggtgcctcac
     1681 gccggaggag ctcggcgaag gcggcgagct cgaggcggcc gtcgggcccg acgagctcgg
     1741 gctgatccag ttctcgtccg ggtcgaccgc ggagcccagg cccgtcgccc tctcgcacgc
     1801 gagcctgctg gcccagctcg cggcccttcg ggcgctcctt cccgcggagg gtgcggtgcc
     1861 gcagaagggc gtctcctggc tgccgctcta ccacgacatg ggcctgatcg gctgcctcct
     1921 ccaggccgtc tactacccgg gcccgctcgt cctgattccc cccgagctct tcctggcgaa
     1981 gccggccctc tggctgcggg caatcgcccg ccaccgcgcg acgatctccg tggcgccgag
     2041 cttcgcgtac gcgctctgct cgcggaaggt gcgggacgag gagctctccg gcgccgacct
     2101 ctcgtcgtgg cggcacgccc tctgcggcgc cgagcccgtc tcgcccgagt cgctcgacga
     2161 tttcgcacgg cgtttcgagc cgttcggctt cgacccgcgg agcctgcggc ccgtctacgg
     2221 cctggccgag gccgcgctcg ccgtcacgtt cacccccgcc ggccgccggg tgaggtcgct
     2281 ccgcgtcgac ccggcgcggc tcgcggccac gggcgacgtc gtggacggga cgcgggcgat
     2341 cgcctcggtc gggacgccgg tcccgggcac ggacgtcgag gtgcgcgacc ccgacggcct
     2401 cgcgctcgag gagcgccgcg
//
LOCUS       DAOTEZ010000569         2414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_36160_length_2414_cov_5.658754, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000569 DAOTEZ010000000
VERSION     DAOTEZ010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36160_length_2414_cov_5.658754"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..472)
                     /locus_tag="P5164_19490"
     CDS             complement(152..472)
                     /locus_tag="P5164_19490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HRY46123.1"
                     /translation="MASIEFNGKTVELNPEGFLADPSIWTEELALLLARQEEGLESLG
                     DQHWTVIRFIREHWLEHDSAPMVRSICKTAGIPLKTVYELFPSGPAKGACKLAGLPKP
                     DGCV"
     gene            551..1924
                     /locus_tag="P5164_19495"
     CDS             551..1924
                     /locus_tag="P5164_19495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRY46124.1"
                     /translation="MTSWIPELSGSKGPTYLAVADAIGAAVRRGELRPGDRLPTHRLL
                     ADLLGVNVSTVTRAYREAARRRLLGGEVGRGTYVLGVASEAALFALEQRPEGGVLDLS
                     PNTPPPLARDPDLSRALGALDDAEAAHLLHYPTAADTLVHARAASAWLGRRGVEAEPE
                     RIVVCAGAQHAMDTAVGAFPEIEEVACEALVYPGLKAVARRLRRRLHAMEMDREGVLP
                     GAFEAACRAGVRLAVLSPTLHNPTSATMGAERRRRIVETARRFDVLLVEEDVYGLLPE
                     GAPPPLAALAPERVLYVTGLSKTVAPGLRLGYLAVPGDLRERWQGASHHTSWYVSPLS
                     SALAARWLTDGTAWRRLVAQRKELAARHRVCARALSGIDWRGARHGPHVWVPLERGSG
                     DAFAKRALSAGVVVVPASVFAAGRSPAGDGVRVSLGAATDRGSLAEALVRLRRLLPAT
                     GRPARCS"
     gene            complement(1965..2384)
                     /locus_tag="P5164_19500"
     CDS             complement(1965..2384)
                     /locus_tag="P5164_19500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HRY46125.1"
                     /translation="MPPSNLAVRLRGRRAHALLALGAFFLVLAVLVFVDPRTARFAPV
                     CPLHYTTGLHCPGCGTGRALHALAHGDLARAVRLNVLAVATIPVFLAVSIRAVLRPAE
                     RLPIPPPWLRIAVYVLLAGYFVARNLPFEPFASLAPR"
BASE COUNT          298 a          855 c          935 g          326 t
ORIGIN      
        1 gccggcggcg gcgtcgccgg ccggcggcgc gtggagcgcg cgccgccccc acgcgcgcgt
       61 ccgaagcgcg aggagggcga gggcgaggag cgcccacgcc gccgcggcgc cgagggcgag
      121 ctccaggggc ccgggcgcgg acatggcttg cctagacgca gccgtcgggc ttcgggaggc
      181 ccgcgagctt gcaggcgccc ttggccgggc cgctcgggaa gagctcgtag accgtcttca
      241 gcggtattcc tgcggtcttg cagatcgagc ggaccatcgg ggccgagtcg tgctcgagcc
      301 agtgctcgcg gatgaagcgg atgaccgtcc agtgctggtc gccgaggctc tccagcccct
      361 cctcctgccg ggcgaggagc agtgcgagct cctcggtcca gatcgacggg tcggcgagga
      421 atccctcggg gttcagctcg acggtcttgc cgttgaattc gatgctcgcc atcgctcctc
      481 cgtgccggcc cctgctctcg ccgggcccga cggccgctat gatgtgtgca ttgaaacagg
      541 gattgtgtgc atgacaagct ggattccgga gctcagcgga tcgaagggcc cgacctacct
      601 cgcggttgca gacgccatcg gcgcggctgt tcgccggggg gagctgcggc ccggagaccg
      661 cctcccgacg caccgtctcc tcgcggacct cctcggcgtc aacgtcagca cggtcacgcg
      721 cgcctaccgc gaggcggcgc gacggcgtct tcttggcggc gaggtgggcc gagggacgta
      781 cgtcctcggc gtggcctccg aggcggctct cttcgcgctc gagcagcggc cggagggggg
      841 cgttctcgac ctctctccga ataccccgcc tccgctcgcc cgcgaccccg atctctcccg
      901 cgcgctcggc gcgctcgacg acgccgaggc ggcgcacctt ctccactacc cgacggcggc
      961 cgacacgctc gtccacgccc gggccgcctc ggcctggctc gggcgccgcg gcgtcgaagc
     1021 ggagccggag aggatcgtcg tctgcgccgg ggcccagcac gcgatggaca cggcggtcgg
     1081 ggcgttcccg gagatcgagg aggtcgcctg cgaggcgctc gtctacccgg gactgaaggc
     1141 ggtcgcccgc cgcctccgcc ggaggctcca cgcgatggag atggatcgcg agggagtcct
     1201 tcccggcgcg ttcgaggcgg cgtgccgcgc cggagtccgg ctcgcggtgc tttcgccgac
     1261 gctgcacaac ccgacgtcgg cgacgatggg ggccgagcgg cgccggagga tcgtcgagac
     1321 ggcccgccgt ttcgacgtcc tcctcgtcga ggaggacgtc tacggcctcc tgccggaggg
     1381 ggcgccgccg ccgctcgcgg cgctcgcccc ggagcgggtg ctctacgtga cgggcctctc
     1441 gaagacggtc gcgcccggcc tgcgcctcgg ctacctcgcc gttccgggtg acctgaggga
     1501 gcgatggcag ggggcgtccc accacacgag ctggtacgtc tcgccgctct cgtccgccct
     1561 cgcggcgcgc tggctgacgg acggcacggc gtggcgccgg ctcgtcgcgc agagaaagga
     1621 gctcgccgct cgacaccgcg tctgtgcccg cgcgctctcg ggcatcgatt ggcgtggagc
     1681 gcggcacgga ccgcacgtct gggttccgct ggagagaggc tccggagacg ccttcgcgaa
     1741 gcgggcgctg tcggccggcg tcgtcgtcgt cccggcgagc gtctttgccg cggggcggtc
     1801 ccccgccggg gacggggtcc gcgtctcgct cggtgccgcg accgaccgcg ggtcgctcgc
     1861 cgaagctctc gtgcgcctgc gaaggctgct ccccgcgacc ggccggcccg ctcgctgctc
     1921 ctgagcggcg acgctgtcgc cggtcgccgc cgggacgcgg cgacctacct cggcgccagg
     1981 ctcgcgaagg gctcgaacgg caggttccgc gcgacgaagt agccggcgag gaggacgtac
     2041 acggcgatcc ggagccaggg tggcgggatc ggcagccgtt cggcggggcg gagcacggcg
     2101 cgaatcgaga ccgcgaggaa gaccgggatc gttgccaccg cgaggacgtt cagccggacg
     2161 gcgcgggcga ggtcgccgtg ggcgagcgcg tggagcgcgc ggccggtgcc gcagccggga
     2221 cagtggaggc ccgtcgtgta gtggaggggg cagacgggcg cgaatcgcgc ggtcctcggg
     2281 tcgacgaaga cgaggacggc gaggacgagg aagaaggctc cgagggcgag gagcgcgtgc
     2341 gcgcgccgcc cccggagccg gacggcgagg ttcgacgggg gcactacttc ttctgcagct
     2401 cgttcccctg agcg
//
LOCUS       DAOTEZ010000570         2391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_36743_length_2391_cov_4.826199, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000570 DAOTEZ010000000
VERSION     DAOTEZ010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36743_length_2391_cov_4.826199"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..1257
                     /locus_tag="P5164_19505"
     CDS             91..1257
                     /locus_tag="P5164_19505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HRY46126.1"
                     /translation="MPALRTVYADHHATTPLRPEALEAMRPWLAGLAANASSLHAPGR
                     AARAAVEGARERIATLLGVRSEELVFTSGGTESDALAVCGGARAARDGEPLRTRVAYS
                     AAEHAAVREAARSLRREGFEPLELPVDGAGLPRQDGLAAANDGRTALLSLILAGNETG
                     ARNGAIAASAAAARAAGALVHTDAVQAVGKVPVRPSELGVDLLSFTGHKLGGPKGAGV
                     LWVRRGVRLSPLFTGGGQERGRRGGTENVAAIAGLAAALETALSRLEEEGRRLAVLRD
                     SFEEGLRERVPGVRVNGLGPAGRLPTASSVTFEGIDGETLLVALDLEGVAASSGSACS
                     SGTPAPSRVLLACGLPEAEVRSTLRFSFGWTTTEEEVERLLEVLPRLVARVRAA"
     gene            1324..1998
                     /locus_tag="P5164_19510"
     CDS             1324..1998
                     /locus_tag="P5164_19510"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRY46127.1"
                     /translation="MIIQCSACNTRYHYDEARFAGAPAKKIRCTKCSTVFEIRNPALD
                     APPPAGFQADETSSISAPMPGSDDFNFDTTVMGPHRKRPVVARPPEPAAAPAPSPRPP
                     AAEADKPPVAAAAAAGRRLRLPDWEKLSLACIAGPEAGRIFEIGKARVVLGRAGVDVL
                     LNDPECSRSHAAIEVSDDKVFLVDLGSTNGTYVADKRISQVELENRSEFDVGSSTLML
                     IRTRKD"
     assembly_gap    2124..2133
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2134..>2391)
                     /locus_tag="P5164_19515"
     CDS             complement(<2134..>2391)
                     /locus_tag="P5164_19515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRY46128.1"
                     /translation="HGLAICPNSAVALAAAGRLRREGAIGRDETVVVVATAHAIKFSG
                     AAVRYHGSGARLANPPRSLPASLEAVAAALGEWEPSTLSGSG"
BASE COUNT          292 a          875 c          910 g          304 t
ORIGIN      
        1 aagcaaggga gagaccgtac cggagtgtac ggccccggcc gggagcgggg tttgcggcgg
       61 cggcagtccc cgctcctaga attcgcgccg atgcccgccc ttcgaacggt ctacgccgac
      121 caccacgcca cgaccccgct ccggccggag gctctcgagg cgatgcgccc ctggctcgcc
      181 gggctcgcgg cgaacgcatc ctcgctccac gccccggggc gtgccgcccg ggcggccgtg
      241 gagggggcga gggagaggat cgcgaccctc ctcggcgtgc gctccgagga gctggtcttc
      301 acctccggcg ggacggagtc ggacgccctg gccgtctgcg gcggggcgcg cgcggcgcgg
      361 gacggagagc cgctccggac ccgcgtcgcg tactcggcgg ccgagcacgc ggcggtgagg
      421 gaggcggcgc ggtccctgcg gcgcgagggg ttcgagccgc tcgagctgcc ggtcgacggg
      481 gcggggttgc cgcgccagga cgggctcgcc gccgcgaacg acggccggac ggccctcctc
      541 tcgctgatcc tcgccggcaa cgagacgggg gcgcggaacg gcgcgatcgc cgcgtcggcc
      601 gccgccgcgc gggccgcggg cgcgctcgtc cacaccgacg ccgtccaggc ggtcggcaag
      661 gtcccggtcc gtccctcgga gctcggagtc gacctcctct ccttcacggg ccacaagctc
      721 ggcggaccga agggggccgg cgtcctctgg gtgagacgcg gcgttcgcct gtcgccgctc
      781 ttcacgggcg gcggccagga acgggggcga cggggcggga cggagaacgt ggccgccatc
      841 gcggggctcg ccgcggcgct cgaaacggcc ctctctcgac tcgaggagga ggggcggagg
      901 ctcgccgtgc tgcgggattc cttcgaggag gggctccggg agagggtccc cggggtgagg
      961 gtcaacggac tcgggccggc cgggaggctc ccgaccgcga gctccgtgac gttcgagggt
     1021 atcgacggcg agacgctcct cgtcgccctc gacctcgagg gggtcgccgc ctcgtccggc
     1081 tcggcctgct cctcggggac gccggcgccg agccgggtcc tcctcgcgtg cggcctgccg
     1141 gaggccgagg tccgctcgac gctccggttc tcgttcggct ggacgacgac ggaggaggag
     1201 gtcgagcgcc tcctggaggt ccttccccgc ctcgtcgcgc gcgtgcgggc cgcttgatcc
     1261 ggggggcgcg ggcgtgtcta atggagcagg tcttcaatcc ggatcagccg cgggggagat
     1321 gcgttgatca tccagtgctc tgcctgcaac acgcgctacc actacgacga ggcccgcttc
     1381 gccggcgctc ccgcgaagaa gatccgttgc acgaagtgct ccacggtctt cgagatccgg
     1441 aacccggccc tggacgcgcc gccgcccgcg gggttccagg ccgacgagac gagctcgatc
     1501 agcgcgccga tgccgggctc cgacgacttc aacttcgaca cgaccgtgat gggcccgcac
     1561 cggaagcggc ccgtggtcgc ccggccgccc gagccggccg ccgccccggc tccgtctccc
     1621 cgccctccgg cggccgaggc ggataagccc ccggtggcgg ccgcggccgc ggccggccgc
     1681 cgcctccgcc tgccggactg ggagaagctg tcgctcgcct gcatcgcggg gccggaagcg
     1741 gggcggatct tcgagatcgg caaggcgcgg gtcgtcctcg gtcgcgccgg ggtcgacgtc
     1801 ctcctgaacg acccggagtg ctcgaggagc cacgccgcga tcgaggtctc cgacgacaag
     1861 gtcttcctcg tcgacctcgg ctcgacgaac gggacgtacg tcgccgacaa gaggatttcc
     1921 caggtcgagc tcgagaaccg ctcggagttc gacgtcggct cctcgacgct gatgctcatc
     1981 cggacgcgga aggactagcc tcccgccccc gaggcgcggc gggcacgccg tgcctcctca
     2041 gcgctccagc tgctcggcga ccgcctcgag ggaggcgggg aggctcctcg ccgggttcgc
     2101 gtgccgcgcg ccgctcccgt ggtnnnnnnn nnnccggacc cggaaagagt agacggctcc
     2161 cactccccga gcgccgcggc gactgcctcg agcgaggccg gaaggctccg tggcgggttc
     2221 gcgaggcgcg cgccgctccc gtggtagcgg acggcggccc ccgagaactt gatggcgtgg
     2281 gcggtcgcga cgacgacgac cgtctcgtcc ctcccgatcg ccccctcgcg ccggagcctc
     2341 ccggccgcgg cgagggcgac ggcggagttg gggcagatcg cgaggccgtg g
//
LOCUS       DAOTEZ010000571         2388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_36824_length_2388_cov_7.354908, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000571 DAOTEZ010000000
VERSION     DAOTEZ010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36824_length_2388_cov_7.354908"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /gene="selD"
                     /locus_tag="P5164_19520"
     CDS             <1..1008
                     /gene="selD"
                     /locus_tag="P5164_19520"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:10..12,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HRY46129.1"
                     /translation="CAGUASKLPPGQLAQVLRVLGDPFPAASRPDVLVGLAEPDDAAV
                     LDLGDGRALILTTDFFTPVVDEPYDFGAIAAANAMSDVWAMGGEPALALNLVAFPPQL
                     SAATLAEVLRGGAEVVRSAGAVIAGGHSIQDKEPKYGLCVVGFGEAGRLLTKGGARPD
                     DGLWLTKPLGTGTVTTALKRGLATEGEVAAAVDSMKTLNRGAARAARAAGAKAATDVT
                     GFGLLGHALEMAEASGVRFRLDWDALPFLPGAARLAADMVFPGGAFGNLEHFGPRLSR
                     TRALSDAEATLLADPQTSGGLLASVPAAGERTFLATLELEGGRAWRIGEVAEGSGIEV
                     R"
     gene            1232..2128
                     /locus_tag="P5164_19525"
     CDS             1232..2128
                     /locus_tag="P5164_19525"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRY46130.1"
                     /translation="MRKLALGAMALLLATVPARAQEDYEGCKDHPLFTRFPNSYIVDC
                     ASSQFDLRKFPVGQMKPGEEVLRSVEVEGPTWRVNFQIKEGTTPPSGLQLMRNFEAAA
                     KRAGGTIEGQWPGWCKANYDSEGMPDMGNSCTSFGLTMKFVKGGKETWAFFQASESDG
                     SYLMTVSEREAMKQEVAVNEIADQLRKDGFVSLYVTFDTGKATISPDSAKLLDDAAAA
                     LKAASELKVEVGGHTDAVGGAEANRKLSQERAQAVMAALVKRGIAAGRMTAKGYGQTV
                     PVADNRTEDGRAKNRRVELVKK"
     gene            2157..>2388
                     /locus_tag="P5164_19530"
     CDS             2157..>2388
                     /locus_tag="P5164_19530"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HRY46131.1"
                     /translation="MDAVTLSRIQFAVSIGFHYIFPLLTIGLGVVLVWLEGWFLKTKD
                     PIYETAARFWTKVFALNFAIGVATGIVMEFQFG"
BASE COUNT          356 a          835 c          873 g          324 t
ORIGIN      
        1 tgcgcggggt gagcgtcgaa gctgcccccc gggcagctcg cgcaggtcct gcgcgtcctc
       61 ggcgacccct tccccgccgc ctcccgcccc gacgtcctcg tcgggctcgc cgagccggac
      121 gacgccgccg tcctcgacct cggggacggg cgcgcgctca tcctgacgac cgacttcttc
      181 acgccggtcg tcgacgagcc gtacgacttc ggcgccatcg ccgcggcgaa cgcgatgagc
      241 gacgtctggg cgatgggggg cgagccggcg ctcgccctga acctcgtcgc gttcccgccg
      301 cagctctccg ccgcgacgct cgccgaggtc ctccgcgggg gagccgaggt cgtgaggtcc
      361 gccggggccg tgatcgcggg cggccactcg atccaggaca aggagccgaa gtacgggctc
      421 tgcgtcgtcg gcttcggcga ggccggccgt ctcctgacga agggaggcgc gcggccggac
      481 gacgggctct ggctgacgaa gccgctcggg acggggacgg tcacgacggc cctcaagcgc
      541 ggcctcgcca cggaaggaga ggtcgccgcg gcggtcgact cgatgaagac gctgaaccgg
      601 ggcgcggcgc gcgccgcgcg ggcggcgggc gcgaaggccg cgaccgacgt caccggcttc
      661 ggcctcctcg gccacgcgct cgagatggcc gaggcgtccg gcgtccgctt ccgcctcgac
      721 tgggacgcgc tccccttcct tcccggagcg gcgcgcctcg cggccgacat ggtcttcccc
      781 ggcggcgcct tcgggaacct cgagcacttc gggccgcgcc tctcgcgcac gcgcgccctc
      841 tccgacgccg aggcgaccct cctcgccgat ccgcagacgt cgggcggcct cctcgcctcc
      901 gtgcccgcgg ccggcgagag gacgttcctc gcgacgctgg agctcgaggg agggcgagcc
      961 tggaggatcg gcgaggtggc ggagggctcc gggatcgagg tccgctgaac cttcggcgcc
     1021 gccttcacgt cccgcggacc gcagctcgtc gtctgcgggg gcgacatctg cgggtgcgct
     1081 ccttgaccct cgcgctccgc gatcggagaa tcgccgacgc gttcgccacg actcgagccg
     1141 aagctctccg ggcccgtcca ggcgcccggg gcggcgattc ggccggcgtg gccgcggacg
     1201 tccccggaag ggacgatcga ggaggaagga aatgagaaag ctcgctctcg gcgccatggc
     1261 gctgctgctc gcgaccgttc ccgcccgggc ccaggaggac tacgagggct gcaaggacca
     1321 cccgctcttc acgcggttcc cgaactcgta catcgtcgac tgcgcgagca gccagttcga
     1381 cctccggaag ttccccgtcg ggcagatgaa gcccggcgag gaggtgctcc ggtccgtcga
     1441 ggtcgagggg ccgacgtggc gcgtcaactt ccagatcaag gaggggacga cccctccgag
     1501 cggcctgcag ctgatgcgca acttcgaggc ggctgcgaag agagccgggg gcacgatcga
     1561 gggccagtgg cccggctggt gcaaggccaa ctacgactcc gaggggatgc ccgacatggg
     1621 gaacagctgc acgagcttcg gcctgacgat gaagttcgtg aaggggggca aggagacctg
     1681 ggccttcttc caggcgagcg agagcgacgg gagctacctg atgaccgtct cggagcgcga
     1741 ggcgatgaag caggaggtcg ccgtcaacga gatcgccgac cagctgagga aggacggctt
     1801 cgtctcgctc tacgtcacgt tcgacacggg caaggcgacg atctcgcccg actcggcgaa
     1861 gctcctcgac gacgccgccg cggcgctgaa ggcggcgtcg gagctgaagg tcgaggtcgg
     1921 cggccacacg gacgccgtcg ggggcgccga ggccaacagg aagctctccc aggagcgcgc
     1981 gcaggcggtg atggctgccc tcgtgaagcg cgggatcgcg gccggccgga tgacggcgaa
     2041 ggggtacggg cagacggtcc cggtcgccga caaccgcacg gaggacggcc gcgcgaagaa
     2101 ccggcgcgtc gagctcgtga agaagtagag gccgcaccgc tccaccgagg tcgccgatgg
     2161 acgccgtcac tctctcgcgc atccagttcg ccgtcagtat cgggttccac tacatcttcc
     2221 cgctgctgac gatcgggctc ggcgtcgtcc tcgtctggct ggaaggatgg ttcctgaaga
     2281 cgaaggaccc gatctacgag acggccgccc ggttctggac gaaggtcttc gcgctcaact
     2341 tcgcgatcgg cgtcgccacc gggatcgtca tggagttcca gttcggga
//
LOCUS       DAOTEZ010000572         2385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_36894_length_2385_cov_6.089270, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000572 DAOTEZ010000000
VERSION     DAOTEZ010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36894_length_2385_cov_6.089270"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1624
                     /locus_tag="P5164_19535"
     CDS             <1..1624
                     /locus_tag="P5164_19535"
                     /inference="COORDINATES: protein motif:HMM:NF021499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY46132.1"
                     /translation="GGRTMRGPRRLSQKLILSLTVVVSLVVVANGVLAVRQAEKQLQA
                     TMLQGAEQISGAIASSTWHAMLADERESAYQVMRTIAAKQGIRSVRIFNKEGRVMFST
                     PPSPVKVVDKKAEACFLCHASEQPLVRVDTPTRARVVKRTDGGRTLAMVTPIYNEPAC
                     SNAACHAHPESQNVLGVLDVGLDMKLVDQQVRRIEIRTALFTSLAVLLVGLCAAAFTR
                     RFVTTPLTRLSEATRQVAEMKLDRPIALTSSEELEALASSFNEMRRRLAVAMAELNEL
                     TRGLEEKVDERTEQLKVAQQKLMQADRLASLGQLSASVAHEINNPLSGVLNLSMLMQR
                     ILKDDGIPKGREAEFRRYLEQVVAETARTGRIVSDLLAFSRRGTPVRSETDLNAIVGR
                     TLSLVSHKLKLMNVEVECALDAALPRVEADSSQLQQVVMNLVLNAGEATRKRETGHVR
                     VSTRATALKKEVVLEVRDDGEGMPPELKERIFEPFFTTKDEGKGVGLGLAVVYGIVRA
                     HDGDIEVESAPGEGTLFRVTLPVHGEAAAAKA"
     gene            1621..2184
                     /locus_tag="P5164_19540"
     CDS             1621..2184
                     /locus_tag="P5164_19540"
                     /inference="COORDINATES: protein motif:HMM:NF033823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin family Zn-dependent
                     metalloprotease"
                     /protein_id="HRY46133.1"
                     /translation="MSVLAVVPIGPSDEEVLAAVEGVVASAFGRETARLAPIPEPAGA
                     FDAARGQWSAPEMLKALHAARPPEADRVLGVTGRDLFVPVLSFVFGQAQMGGPTAVIS
                     LARISQAFSGLPEDRPLLLARARKEAVHELGHTFGLVHCPDPACPMSLSTGVVQVDRK
                     GESLCASCAALVKERIPAAGPPEGRTG"
     gene            2181..>2385
                     /locus_tag="P5164_19545"
     CDS             2181..>2385
                     /locus_tag="P5164_19545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY46134.1"
                     /translation="MKATWRILVIDDEAVMRESLAAWLREDGYEVDTAGSGREGIEKA
                     RGADYAIYFVDLKMPGGIDGIETM"
BASE COUNT          365 a          830 c          864 g          326 t
ORIGIN      
        1 gggcggacgg acgatgcgcg gaccgaggcg actctcccag aagctcatcc tctcgctgac
       61 ggtcgtcgtc tcgctcgtcg tcgtggcgaa cggtgtcctc gccgtccggc aggccgagaa
      121 gcagctccag gcgacgatgc tccagggggc ggagcagatc tcgggggcca tcgcgagctc
      181 gacctggcac gcgatgctcg ccgacgagcg cgaatcggcc taccaggtga tgaggacgat
      241 cgccgccaag caggggattc ggagcgtcag gatcttcaac aaggaggggc gcgtcatgtt
      301 ctcgacgccc ccctccccgg tgaaggtcgt cgacaagaag gcggaggcct gcttcctctg
      361 tcacgcctcc gagcagccgc tcgtgagggt cgacacgccg acccgcgccc gcgtcgtgaa
      421 gcgcacggac ggcggccgga ccctcgcgat ggtcaccccg atctacaacg agccggcgtg
      481 cagcaatgcc gcctgccacg cccacccgga gagccagaac gtcctcggcg tcctggacgt
      541 cggcctcgac atgaagctcg tcgatcagca ggtgcgccgg atcgagatcc ggaccgcgct
      601 cttcacctcg ctcgcggtcc tcctcgtcgg cctctgcgcg gcggcgttca cgcggcggtt
      661 cgtcacgacg ccgctcacgc ggctctcgga ggcgacccgg caggtggcgg agatgaagct
      721 cgaccggccg atcgcgctca cctcgagcga ggagctggag gcgctcgcga gctccttcaa
      781 cgagatgcgt cgccggctcg ccgtggcgat ggcggagctg aacgagctca cgcgggggct
      841 cgaggagaag gtcgacgaga ggaccgagca gctgaaggtg gcccagcaga agctgatgca
      901 ggccgaccgg ctcgcctccc tcggccagct ctcggccagc gtcgcccacg agatcaacaa
      961 cccgctctcc ggcgtcctga acctctcgat gctgatgcag cggatcctca aggacgacgg
     1021 catcccgaag gggcgggagg cggagttccg gcggtacctc gagcaggtcg tcgccgagac
     1081 ggcccggacc gggcgcatcg tctccgacct cctcgccttc tcccggcggg ggacgcccgt
     1141 ccgctccgag accgacctga acgcgatcgt gggccggacc ctctcgctgg tctcccacaa
     1201 gctcaagctg atgaacgtcg aggtcgaatg cgccctcgac gccgcgctcc cgcgcgtcga
     1261 ggccgactct tcgcagctcc agcaggtcgt catgaacctc gtcctgaacg cgggcgaggc
     1321 gacccggaag agggagacgg gccacgtccg cgtctccacg cgggcgacgg cgctgaagaa
     1381 ggaggtcgtc ctggaggtgc gcgacgacgg cgaggggatg ccgcccgagc tgaaggagcg
     1441 gatcttcgag ccgttcttca cgacgaagga cgaggggaag ggagtcgggc tcgggctcgc
     1501 cgtcgtctac ggcatcgtcc gggcccacga cggcgacatc gaggtcgaga gcgcgcccgg
     1561 ggaggggacc ctcttccgcg tcacgctccc ggtccacgga gaggcggcgg ccgcgaaggc
     1621 gtgagcgtcc tcgccgtggt cccgatcggc ccctccgacg aagaggtcct ggccgccgtc
     1681 gaaggcgtcg tcgcgtccgc cttcgggcgc gagacggcgc ggctcgcgcc gatcccggag
     1741 ccggccgggg cattcgacgc cgcgcggggc cagtggagcg ctccggagat gctcaaggcg
     1801 ctccacgcgg cccggccgcc ggaggccgat cgggtcctgg gcgtgaccgg gcgggacctc
     1861 ttcgtcccgg tcctcagctt cgtcttcggt caggcgcaga tgggcggccc gacggccgtc
     1921 atctccctcg cccggatctc gcaggcgttc tccgggctcc ccgaggaccg gccgctcctc
     1981 ctcgcgcggg cccgcaagga ggccgtccac gagctcgggc acacgttcgg cctcgtccac
     2041 tgcccggacc cggcctgccc gatgtcgctc tcgaccggcg tcgtccaggt cgatcgcaag
     2101 ggagaatcgc tctgcgcctc gtgcgcggcg ctcgtgaagg aacggatccc ggcggcgggg
     2161 ccgccggaag ggaggacggg atgaaggcga cgtggcggat cctcgtcatc gacgacgagg
     2221 cggtcatgcg cgagtcgctc gcggcctggc tccgcgagga cgggtacgag gtcgacacgg
     2281 cgggctcggg ccgggagggg atcgagaagg cgcgcggggc cgactacgcg atctacttcg
     2341 tcgacctgaa gatgccgggg gggatcgacg gcatcgagac gatga
//
LOCUS       DAOTEZ010000573         2362 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_37482_length_2362_cov_6.373645, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000573 DAOTEZ010000000
VERSION     DAOTEZ010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37482_length_2362_cov_6.373645"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..581)
                     /locus_tag="P5164_19550"
     CDS             complement(147..581)
                     /locus_tag="P5164_19550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46135.1"
                     /translation="MTVKWIGDAAQELGLSPLDAVEILASRQEYPMNGLLDEDRFKTL
                     RLMRGSKRLEASGSQPMPVPDRLASPSTVPIPTPPPQAHAAPPPARPNTTPPPVPEPF
                     APAPPPVRPPVGERTSVTAPLQTIPGREGEKTVILSPIEPKE"
     gene            complement(594..1514)
                     /locus_tag="P5164_19555"
     CDS             complement(594..1514)
                     /locus_tag="P5164_19555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46136.1"
                     /translation="MGLLKDFLRIDADRRSALATVRQEAQSHRLRIDELGAEARRKRD
                     EIGMLEEELRRLAEDVARTEELLLDLEARREAHDRDTHERKVDAVRSGLAHDRADGYR
                     IAEEFRQLRSGFEGERARLLAEADTGRMMDNFFQIEAFLKDTGTPIPDAARKALMKER
                     QDLMARIGPLVAPPPAPDGVFKATVVYSTLEEGEPTAVVAVGLPDEAEPSGPHDLAAL
                     LLYGSYASVVEKIGPGVPRPRRADGVVVYEQPAGSRPADEVALDLFLAVEDGLRKAAA
                     AAGVPCELTGVFLEPEIASAVFPRGDARGI"
     gene            1649..2044
                     /locus_tag="P5164_19560"
     CDS             1649..2044
                     /locus_tag="P5164_19560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006341091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HRY46137.1"
                     /translation="MDGKPLGEAEIIARKLEKAGVDVARRHVFLCADRENPKCADAER
                     AMAAWAHLKDGLKRRGLSEKGGVLRTRVGCLRICEGGPIAVVYPEGIWYRACDPPVLD
                     RIMDEHLVGGRPVEEFVIARHALTDGGEE"
     gene            complement(2022..>2362)
                     /gene="miaE"
                     /locus_tag="P5164_19565"
     CDS             complement(2022..>2362)
                     /gene="miaE"
                     /locus_tag="P5164_19565"
                     /inference="COORDINATES: protein motif:HMM:NF017939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 -
                     tRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl
                     adenosine)-hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase
                     MiaE"
                     /protein_id="HRY46138.1"
                     /translation="RAGREPEQGLDLLLCGALVEARSHERFLALVPRLAAEPRLAKLY
                     VELAAAEERHGPAYVELAERHAGKETTAARLEALLDVEARAIAGAPASPVAVHSGEAP
                     TPAVTPRRRP"
BASE COUNT          300 a          835 c          912 g          315 t
ORIGIN      
        1 ggcacacgtt cgaccggaaa cagatcgagc tcgcacgcga ggtgcgggag cttctgctcc
       61 ccgctccggg acgtctcgcc ggccgggccg ggacggctgc cttcaggtcg tgacggcggc
      121 ccgccgcccg ccgggcggcg ccgggctcac tccttcggct cgatcggcga gaggatgacc
      181 gtcttctccc cctcgcgccc cggaatggtc tggagcggcg ccgtcacgga ggtcctctca
      241 ccgaccggcg gccggacggg cggaggggcc ggagcgaacg gctcgggaac cggcggcggg
      301 gtcgtgttgg gacgcgcggg gggaggagcg gcgtgcgcct gcggcggagg cgtcgggatc
      361 gggaccgtcg agggggaggc gaggcggtcg ggaaccggca tcggctgaga cccggacgcc
      421 tcgagccgct tgctcccgcg catcagccgg agggtcttga accggtcctc gtcgaggagc
      481 ccgttcatgg ggtactcctg gcgcgacgcg aggatctcga cggcgtcgag aggggagagg
      541 ccgagctcct gggcggcatc gccgatccac ttgacggtca tggcagcctc cggtcagatg
      601 ccgcgggcgt cgccgcgggg aaagaccgcc gaagcgatct cgggctcgag gaagactccg
      661 gtcagctcgc acgggacgcc ggccgccgcg gcggccttcc gcaggccgtc ctcgacggcg
      721 aggaagaggt cgagcgcgac ctcgtcggcg gggcgggagc cggccggctg ctcgtagacg
      781 acgacgccgt ccgcgcggcg aggccgcggc actccggggc cgatcttctc gacgaccgac
      841 gcgtaagacc cgtacaggag aagggccgcg aggtcgtgcg gcccggacgg ctcggcctcg
      901 tcgggaaggc cgaccgccac gacggcggtc ggctcgccct cctcgagagt cgagtagacg
      961 acggtcgcct tgaagacgcc gtcgggcgcc ggaggcgggg cgacgagcgg gccgatccgc
     1021 gccatcaggt cctgccgctc cttcatcagc gccttgcggg ccgcgtcggg gatcggcgtc
     1081 ccggtgtcct tgaggaacgc ctcgatctgg aagaagttgt ccatcatccg gccggtgtcg
     1141 gcctcggcca ggaggcgtgc gcgctcgccc tcgaagccgc tgcgcagctg ccggaactcc
     1201 tccgcgatcc gatacccgtc ggcgcggtcg tgcgcgaggc ccgagcggac cgcgtcgacc
     1261 ttccgttcgt gggtgtcccg gtcgtgggcc tcgcgtcgcg cctccaggtc gaggaggagc
     1321 tcttccgtcc gggccacgtc ctcggcgagc cggcgcagct cctcttccag cattccgatc
     1381 tcgtcccgct tgcgtcgcgc ctccgcgccg agctcgtcga tccggagccg gtgggactgc
     1441 gcttcctggc gcaccgtcgc gagggcggaa cgccggtcgg cgtcgatccg gaggaagtcc
     1501 ttcaggaggc ccatcgccat ctccttgagt cggaagtccc ggggagccgg ggaagtctat
     1561 accggggcgc cgggagactt cgccgggtgc gcctccgctg cgcggcgctc cggcgctcgc
     1621 ctacacttgc cgcccgacgg agggcgccat ggacggcaag ccgctcggag aggccgagat
     1681 catcgcccgg aagctcgaga aggccggcgt cgacgtcgcc cgccgccacg tcttcctctg
     1741 cgcggaccgc gagaacccga agtgcgccga cgcggagcgc gcgatggccg cgtgggcgca
     1801 cctgaaggat ggcctgaagc ggcggggcct ttcggaaaag gggggcgtcc tccggacccg
     1861 ggtcggctgc ctccggatct gcgaaggggg gccgatcgcc gtcgtctacc ccgaggggat
     1921 ctggtaccgg gcctgtgatc cgccggtcct cgaccgaatc atggacgagc acctcgtcgg
     1981 cgggcgtccc gtcgaggagt tcgtcatcgc ccggcacgcg ctcacggacg gcggcgagga
     2041 gtgacggccg gggtcggcgc ctctccggaa tggacggcga ctggcgaggc cggggcgccc
     2101 gcgatcgcgc gcgcctcgac gtcgaggagc gcctcgagcc gggccgccgt cgtctccttc
     2161 cccgcgtgcc gctcggcgag ctcgacgtac gccggcccgt gccgctcctc ggccgccgcg
     2221 agctcgacgt agagcttcgc gaggcgcggc tccgcggcga gccgcgggac gagcgcgagg
     2281 aacctctcgt ggctccgcgc ctcgacgagg gcgccgcaga ggaggaggtc gagcccctgc
     2341 tcgggctcgc gcccggcgcg gc
//
LOCUS       DAOTEZ010000574         2347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_37881_length_2347_cov_4.376963, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000574 DAOTEZ010000000
VERSION     DAOTEZ010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37881_length_2347_cov_4.376963"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1533
                     /locus_tag="P5164_19570"
     CDS             <1..1533
                     /locus_tag="P5164_19570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY46139.1"
                     /translation="LAGFALTGLPLRPAEDPLRSPAGPGRLLRFLFANETPAETILFR
                     ARLPFVALLAALLLAVRGEARRRWGPWAGVAALAFAALEPNLNAHAGVVHTDLAVTLF
                     VVLSLSPLARLARPEAGRAAPLLGLLWGLAFLSKYSAPLLALATLPFLAADTTPGRQD
                     LGRLLRRLAAAAAIALAVTLAGFAWACRGQSAEDRRAIAVERLELKGRSPAAARLAVR
                     AGDLLPPLGNVLTGALSVALQSEVGAGVNYFLGAVSREGSPWYFPVALAVKTPLGLLL
                     GVGVALSARAGRRTAAAAGASLLLFLLFSSRTTYNIGVRHALLAFPLLAIVAGAAASG
                     LTDLSARRSARLRLGATLGALLAASLEAATAHPHPFSFFNALAGGPDGGRRFFADSSL
                     DWGQDLLRLKALAPSLAPDGLPTVAFGGDLPSRHAPALRPPAAGEEDRPGAVFAIGEA
                     PFALGPELLRAKGATGDAERLESIRTALRERGRRIGTVGGSIGLWRIESPPARREPAR
                     AS"
     gene            complement(1530..>2347)
                     /locus_tag="P5164_19575"
     CDS             complement(1530..>2347)
                     /locus_tag="P5164_19575"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY46140.1"
                     /translation="DLEGAGKSVRFVSHPGLVVVLLGARFDLAGPLRSREVRQAIYFA
                     IDPRELAEESGIEATPVDQVVPPSVFGYLPDGGEPRPDRERARELLRRAGLGDGFDVT
                     LDMAEAFAPNVGRVLAEQLSRVGIRTTVVGLPWSVLSARLERGESPFFSIGWSCNGDA
                     SHLFDALLHTRQGRSWGVSNFGGYANPRLDEAIERAGAILSPARRLEALHEAMRIALD
                     DLPLIPLFNRRRTYGVDARLRFEPRLNGQVILRELSWGGVPDATPASAGLGGF"
BASE COUNT          246 a          957 c          820 g          324 t
ORIGIN      
        1 ctcgccggct tcgcgctcac cggccttccg cttcgcccgg ccgaggaccc gctccgctcc
       61 cctgccggcc ccgggcgcct cctccgcttc ctcttcgcga acgagacccc ggccgagacg
      121 atcctcttcc gggcccggct ccccttcgtc gctctcctcg cggcgctcct gctcgccgtc
      181 cggggcgagg cgcggagacg ctgggggccc tgggcggggg tcgcggccct cgccttcgcc
      241 gccctcgagc cgaacctgaa cgcccacgcc ggggtcgtcc acaccgacct cgccgtcacg
      301 ctcttcgtcg tcctctccct ctcgccgctc gcgcgcctcg cccgtcccga agccggacgc
      361 gccgcgcctc tcctcggcct cctctggggg ctcgccttcc tctcgaagta cagcgccccg
      421 ctcctcgccc tggcgacgct cccgttcctc gccgccgaca cgacgcccgg acggcaggac
      481 ctcgggcgcc tcctccgccg cctcgcggcc gccgccgcga tcgccctcgc ggtgaccctc
      541 gccggcttcg cctgggcctg ccgcggccag tcggccgagg accggcgggc gatcgccgtc
      601 gagaggctcg agctgaaggg gcgctccccg gctgcagcgc gcctcgcggt ccgcgccgga
      661 gacctcctgc cgccgctcgg gaacgtcctg acgggcgcgc tctcggtcgc gctccagagc
      721 gaggtcggtg cgggcgtgaa ctacttcctg ggcgccgtgt cgcgcgaggg ctcgccctgg
      781 tacttccccg tcgccctcgc ggtcaagacg ccgctcggcc tcctcctcgg cgtcggcgtc
      841 gcgctctccg cgcgcgcggg gcgccgcacc gcggccgccg ccggcgcctc gctcctcctg
      901 ttcctcctct tctcgtcgcg gacgacgtac aacatcggcg tgaggcacgc gctcctcgcc
      961 ttccctctcc tcgcgatcgt ggcgggcgcc gcggcgtcgg gcctgacgga tctgtcggcc
     1021 cgtcgctccg cgaggctccg gctcggagcg acgctcggcg cgctcctcgc cgcctccctc
     1081 gaggcggcga cggcgcaccc gcacccgttc tcgttcttca acgccctcgc gggagggccc
     1141 gacgggggcc ggcgcttctt cgccgacagc agcctcgact ggggacagga cctcctccgg
     1201 ctgaaggccc tggccccctc gctcgctccg gacggcctcc cgacggtcgc cttcggcggc
     1261 gacctcccct cgcgccacgc gccggccctt cgcccgccgg ctgcgggtga ggaggaccgt
     1321 cccggcgccg tcttcgcgat cggagaggcg ccgttcgcgc tcggtcccga gctgctccgg
     1381 gcgaaggggg cgactggaga cgccgagcgg ctcgagtcga tccggacggc tctccgcgag
     1441 cgcggccgcc gcatcgggac cgtgggaggg tcgatcgggc tctggcggat cgagagccct
     1501 cccgcgcgcc gggagcccgc tcgggcgtcc tagaacccgc cgagcccggc cgacgccggg
     1561 gtggcgtccg ggacgcctcc ccaggagagc tcgcggagga tgacctgccc gttcaggcgc
     1621 ggctcgaagc gaagccgcgc gtcgaccccg taggtcctcc gccggttgaa gagcgggatg
     1681 aggggcaggt cgtcgagggc gatcctcatc gcctcgtgca gcgcctccag cctccgggcc
     1741 ggggagagga tcgccccggc ccgctcgatc gcctcgtcga gccgggggtt cgcgtagccg
     1801 ccgaagttcg agactcccca gctccggccc tgccgcgtgt ggaggagggc gtcgaagagg
     1861 tgcgacgcgt cgccgttgca cgaccagccg atcgagaaga aggggctttc cccgcgttcg
     1921 agccgggcgg agaggaccga ccacgggagc ccgacgaccg tcgtccggat gccgacccgg
     1981 gagagctgct cggcgaggac gcggccgacg ttcggggcga acgcctccgc catgtcgagc
     2041 gtcacgtcga agccgtctcc cagcccggcc cggcgcagca gctcccgcgc ccgctcgcgg
     2101 tccgggcgcg gctcgccgcc gtccggcagg tagccgaaga ccgacggcgg aacgacctgg
     2161 tcgacgggcg tcgcctcgat cccggattcc tcggcgagct cccgggggtc gatggcgaag
     2221 tagatcgcct gccgcacctc gcgcgaccga agagggcccg cgaggtcgaa ccgcgccccg
     2281 aggaggacga cgacgaggcc cggatggctg acgaaccgga cgctcttccc ggcgccttcg
     2341 aggtcct
//
LOCUS       DAOTEZ010000575         2344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_37957_length_2344_cov_5.197029, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000575 DAOTEZ010000000
VERSION     DAOTEZ010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37957_length_2344_cov_5.197029"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..787
                     /locus_tag="P5164_19580"
     CDS             65..787
                     /locus_tag="P5164_19580"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRY46141.1"
                     /translation="MSWNTPAGRVVWAGPPGWSRLPNVTRALLVLTAAGYLGGVLRLA
                     PMRLLSLFPERIWPALELWRLVTYPLVVLGIFNVLFGLLILWMFGWELEVTFGSRKFG
                     LFVLSAVVVSALLGTAVALLFPAAGAMAGAGLSGLLTAMIVAWALLGPRLPANLFGIL
                     PMTRRGFALLALVLVVFGELETSRSLPQLLFLLGGLPVAWLFVRSTRRGGGGSVRPFR
                     RRWNPFRRRRFTVVEDRSGRVH"
     gene            835..1350
                     /locus_tag="P5164_19585"
     CDS             835..1350
                     /locus_tag="P5164_19585"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HRY46142.1"
                     /translation="MRPSITPLEIRKRTFPIRFRGLDRNEVAQYLELVGDDLEELMRT
                     LDDLERENSRLKDEVAHHRQTEAVLKETLVMAQRNAEALRADSEREADRILAEANRQG
                     EKLVQQALEKAAEKEKRIRELRVERKNFHLKLQGMLDMFQQVLNFDKEEEDLDASVSV
                     MRPKRKDGEAV"
     gene            1347..>2344
                     /gene="hutI"
                     /locus_tag="P5164_19590"
     CDS             1347..>2344
                     /gene="hutI"
                     /locus_tag="P5164_19590"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HRY46143.1"
                     /translation="MSRLVVRHLSQLATPQGKAARTGAAQGRISRVEDAALVCEDGRI
                     SWIGTDADFVAAHGEAPRADDVVLDGRGKTALPGFVDPHTHIPWAGYRESEFNERLKG
                     KTYAEIAAAGGGIVSTVAATRAASVEELAAVTGERLDRMLLHGTTFCEAKTGYGLDLA
                     SERKQLAALKAAAAGHPVGVVATALPGHEVPLERRGSEAQKARYVAECCDEILPALAA
                     DGARFVDVFCEHGVFDLAESRRILEAGKAVGLAPRLHADELTPLGGARLAAEVGALSA
                     DHLLFAQPDGIHAMAKAGVVATLLPGTSVFLRMNRYAPAREMVSAGVAVALGTDCNP"
BASE COUNT          312 a          869 c          817 g          346 t
ORIGIN      
        1 acgatcgtga gggtcgggac ggcccttttc ggctcccgct cctgaccctg ctacgctcgc
       61 gtcgatgagc tggaacacgc ccgcggggcg cgtcgtctgg gccggccctc ccggctggtc
      121 gcgcctcccc aacgtcacgc gcgcgctcct cgtcctgacg gcggcggggt acctcggcgg
      181 cgtcctgcgc ctggcgccga tgcgcctcct ctcgctcttc ccggagagga tctggccggc
      241 gctcgagctc tggcggctcg tcacctatcc gctcgtcgtc ctcgggatct tcaacgtcct
      301 gttcgggctc ctcatcctct ggatgttcgg gtgggagctg gaggtgacgt tcgggtcgcg
      361 gaagttcggc ctcttcgtcc tctcggccgt cgtcgtctcc gccctcctcg ggacggccgt
      421 cgcgctcctc tttcccgcgg cgggggcgat ggcgggggcg gggctctcgg gcctcctgac
      481 ggcgatgatc gtcgcctggg cgctcctcgg cccgcgactc cctgcgaacc tcttcggcat
      541 cctcccgatg acgcggcgcg gcttcgccct cctcgcgctc gtcctcgtcg tcttcggcga
      601 gctcgagacg tcgcgctccc tcccccagct cctcttcctc ctcggcggcc tcccggtcgc
      661 ctggctcttc gtccgctcga cgcgccgcgg cggcggcggc tccgtccgcc cgttccggcg
      721 ccgctggaac cccttccggc gccgccgctt cacggtcgtg gaggaccgga gcggacgcgt
      781 tcactagctc cgcgtgagga gggggcgcgc cgcaaagggc ttagaatgaa cgcgatgaga
      841 ccctccatca cccctctgga gatccgcaaa cggacgttcc cgatccggtt ccggggcctc
      901 gaccggaacg aggtcgccca gtacctcgag ctcgtcggcg acgacctcga ggagctcatg
      961 cggacgctcg acgacctgga gcgcgagaac tcgcgcctga aggacgaggt ggcgcaccac
     1021 cgtcagaccg aagccgtcct gaaggaaacc ctcgtgatgg cgcagcggaa cgcggaggcg
     1081 ctccgcgccg attccgagcg cgaggcggac cggatcctcg cggaggcgaa ccgccagggc
     1141 gagaagctcg tgcagcaggc gctcgagaag gccgccgaaa aggagaagag gatccgcgag
     1201 ctccgggtgg agcggaagaa cttccacctg aagctccagg ggatgctcga catgttccag
     1261 caggtactga acttcgacaa ggaagaggag gacctcgacg cctcggtctc cgtcatgcgg
     1321 ccgaagagga aggacggaga ggccgtttga gccgcctcgt cgtccggcac ctctcccagc
     1381 tcgccacgcc ccaggggaag gccgcccgga ccggagccgc ccaggggcgg atctcgaggg
     1441 tcgaggacgc cgccctcgtc tgcgaggacg gtcgcatctc ctggatcggt acggacgccg
     1501 acttcgtcgc cgcgcacggc gaggcgcccc gcgccgacga cgtcgtcctc gacgggcgcg
     1561 ggaagaccgc gcttcccggc ttcgtagacc cgcacacgca catcccgtgg gccggctacc
     1621 gcgagagcga gttcaacgag cggctgaagg ggaagacgta cgccgagatc gcggcggcgg
     1681 gcggcggcat cgtctcgacc gtggcggcga cgcgggccgc ttccgtcgag gagctcgcgg
     1741 ccgtcaccgg cgagcggctc gaccggatgc tcctccacgg gacgaccttc tgcgaggcga
     1801 agacgggata cggcctcgac ctcgcgagcg agcggaaaca gctcgcggcg ctgaaggccg
     1861 cggccgccgg ccacccggtc ggcgtcgtcg cgacggcgct cccggggcac gaggtgccgc
     1921 tcgaacgccg cggctccgag gcgcagaagg cacgttacgt cgccgagtgc tgcgacgaga
     1981 tcctgccggc cctcgcggcc gacggcgcac gcttcgtcga cgtcttctgc gagcacggcg
     2041 tcttcgacct cgcggagtcc cgccggatcc tcgaggcggg gaaggccgtc ggcctcgcgc
     2101 cccgcctcca cgccgacgag ctgacgcctc tcggcggggc gcgcctcgcg gcggaggtcg
     2161 gcgccctctc ggccgaccac ctcctcttcg cgcagcccga cggcatccac gcgatggcga
     2221 aagcgggggt cgtcgcgacg ctcctccccg ggacgtcggt cttcctccgg atgaaccgct
     2281 acgcgccggc gcgcgagatg gtctcggccg gcgtcgcggt cgccctcggg accgactgca
     2341 accc
//
LOCUS       DAOTEZ010000576         2329 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_38315_length_2329_cov_6.254178, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000576 DAOTEZ010000000
VERSION     DAOTEZ010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38315_length_2329_cov_6.254178"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="P5164_19595"
     CDS             <1..1380
                     /locus_tag="P5164_19595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRY46144.1"
                     /translation="FKGMKYTIQVAPEDCTGCTLCAVVCPAKDKGNPRHKALDMTPQA
                     PLREAERENFSFFLSLPEPDRTKLKIDVKGSQFMQPLFEFSGACLGCGETPYIKLMTQ
                     LYGDRLLIANATGCSSIYGGNLPTTPYTVNADGRGPAWANSLFEDNAEFGFGMRLALD
                     KQAEHAAELVKGLAGTVGDELVAGLLTADQSTEQGIVHQRERVAALKAKLAGAKDLPS
                     RRLLDVADYLVKKSLWIVGGDGWAYDIGYGGLDHVLAMGRNVNVLVLDTEVYSNTGGQ
                     ASKSTPIGAAAKFAMAGKSAGKKDLALMAIAYGNVYVARIAFGAKDAQTVKAFQEAES
                     YDGPSLLLAYSHCIAHGYDLSLGLEQQKTAVDSGYWPIFRWDPRRVAAGQPGLVLDCS
                     APKTDVGAFMANETRFRMLDKTNPERAKMLLELAREQAASRWAVYENLARGVEPAAAV
                     AAAQPAPKA"
     gene            1402..>2329
                     /locus_tag="P5164_19600"
     CDS             1402..>2329
                     /locus_tag="P5164_19600"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HRY46145.1"
                     /translation="MDLSTTYLGLKLPHPVMPGASPMVDKIDLVKRMEDAGASAIVMH
                     SLFEEQLMREELATYHHIDVHADSFAEAMTYLPRPDEFNLGPDQYLEQLVRIKQSVDL
                     PVIASLNGFTSGGWIRYAKLMQDAGANALELNVYYLATNADEGGEEVEQRTIDILKAV
                     KQAVTIPVSIKLSPFFSSMASMARRLDEAGADGLVLFNRFYQPDIDVESLEVEPALKL
                     SDSSELNLRLRWLAVVSGRVRASLAVSGGVHTHLDAVKAVMTGAHAVQVVSAILQQGP
                     DVLRRIREGLTQWLEEHEYDSLEQMRGSMNLLR"
BASE COUNT          389 a          856 c          756 g          328 t
ORIGIN      
        1 ttcaagggga tgaagtacac gatccaggtc gccccggagg actgcacggg ctgcacgctc
       61 tgcgccgtcg tctgcccggc gaaggacaag gggaacccgc gtcacaaggc gctcgacatg
      121 acgccccagg cgccgctccg cgaggccgag cgcgagaact tcagcttctt cctctcgctg
      181 cccgagcccg accggacgaa gctgaagatc gacgtgaagg gctcgcagtt catgcagccc
      241 ctcttcgagt tctccggcgc ctgcctcggc tgcggcgaga cgccctacat caagctgatg
      301 acgcagctct acggcgaccg gctcctgatc gcgaacgcca cgggctgctc ctcgatctac
      361 ggcggcaacc tcccgacgac gccctacacg gtcaacgccg acgggcgggg cccggcctgg
      421 gcgaactccc tcttcgagga caacgccgag ttcggcttcg gcatgcgcct cgccctcgac
      481 aagcaggccg agcacgccgc cgagctcgtg aaggggctcg ccggcaccgt gggcgacgag
      541 ctcgtcgcgg gcctcctgac ggccgaccag tcgaccgagc aggggatcgt ccaccagcgc
      601 gagcgggtcg ccgccctgaa ggcgaagctc gccggcgcga aggacctgcc gtcccgccgc
      661 ctcctcgacg tcgccgacta cctcgtgaag aagagcctct ggatcgtcgg cggcgacggc
      721 tgggcctacg acatcggcta cggcggcctc gaccacgtcc tcgccatggg gcggaacgtg
      781 aacgtcctcg tcctcgacac cgaggtctac tccaacaccg gcggccaggc ctcgaagtcg
      841 acgccgatcg gcgccgccgc gaagttcgcg atggcgggca agtcggccgg caagaaggac
      901 ctcgccctga tggcgatcgc ctacgggaac gtctacgtcg cgaggatcgc gttcggcgcg
      961 aaggacgccc agacggtcaa ggccttccag gaggccgaga gctacgacgg gccgtcgctc
     1021 ctcctcgcct acagccactg catcgcccac ggctacgacc tgtcgctcgg cctcgagcag
     1081 cagaagacgg ccgtcgattc cggctactgg ccgatcttcc ggtgggaccc gcgccgggtc
     1141 gcggcgggcc agccggggct cgtcctcgac tgctccgccc cgaagaccga cgtcggcgcg
     1201 ttcatggcca acgagacgcg cttccggatg ctcgacaaga cgaaccccga gcgggccaag
     1261 atgctcctcg agctcgcccg cgagcaggct gcgtcgcggt gggccgtcta cgagaacctc
     1321 gcccgcgggg tcgagccggc cgccgcggtg gccgccgccc agcccgcgcc gaaggcctga
     1381 cgaaaggacg aggagcagag aatggacctc tccacaacct acctcggtct gaagctcccc
     1441 cacccggtga tgccgggcgc ctcgccgatg gtcgacaaga tcgacctcgt caagcggatg
     1501 gaggacgccg gggcgtcggc catcgtcatg cactccctct tcgaggagca gctcatgagg
     1561 gaggagctcg cgacctacca tcacatcgac gtccacgccg actcgttcgc cgaggcgatg
     1621 acgtacctcc cccggcccga cgagttcaac ctcgggccgg accagtacct cgagcagctc
     1681 gtgcggatca agcagtccgt cgacctgccc gtcatcgcct cgctcaacgg cttcacctcg
     1741 ggcggctgga tccgctacgc gaagctgatg caggacgccg gggcgaacgc cctcgagctg
     1801 aacgtctact acctcgccac gaacgccgac gaggggggcg aggaagtcga gcagcggacg
     1861 atcgacatcc tcaaggcggt caagcaggcc gtcacgatcc cggtctcgat caagctctcc
     1921 cccttcttct cgtcgatggc cagcatggcg aggaggctcg acgaggcggg cgccgacggc
     1981 ctcgtcctct tcaaccggtt ctaccagccc gacatcgacg tcgagagcct cgaggtggag
     2041 ccggcgctga agctctccga ctcctccgag ctcaacctgc ggctccgctg gctggccgtc
     2101 gtctcggggc gcgtccgcgc ctcgctcgcg gtctcgggcg gcgtccacac ccacctcgac
     2161 gcggtcaagg cggtcatgac cggcgcccac gccgtccagg tcgtctcggc gatcctccag
     2221 caggggcccg acgtcctccg gcggatccgc gaggggctga cgcagtggct cgaggagcac
     2281 gagtacgact cgctcgagca gatgcgcggt tcgatgaacc tcctccgct
//
LOCUS       DAOTEZ010000577         2323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_38488_length_2323_cov_6.800705, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000577 DAOTEZ010000000
VERSION     DAOTEZ010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38488_length_2323_cov_6.800705"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="P5164_19605"
     CDS             complement(<1..236)
                     /locus_tag="P5164_19605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HRY46146.1"
                     /translation="MTSSTLSVRPSSARPVPGTVRRAAFAGAAAALLAATTLHAGEAP
                     KKPLTFGASPQVARGQYLVGIMGCDDCHTPMKMGA"
     gene            423..899
                     /locus_tag="P5164_19610"
     CDS             423..899
                     /locus_tag="P5164_19610"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRY46147.1"
                     /translation="MAVPGKLASLLEGLVAVHVATRDAALVPDEVMAAAALLELDGRS
                     LTVYLPDATAGTALANLRDNGEVAVVLSQPLTHRTVQLKGRVETLRAAREEEREAVER
                     FAAAFDAEVAAIGLPPAALRRRTRWPCAAATFAVREAYEQTPGPRAGEPLAPEGPR"
     gene            896..2266
                     /locus_tag="P5164_19615"
     CDS             896..2266
                     /locus_tag="P5164_19615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRY46148.1"
                     /translation="MIAKLPALARCFQGILPGALATCAPDGTPNVTYLSQIRQVDERH
                     VAISRQFFNKTSRNLEANPRATATVFDPVTFEQWKLGLRYVRSETDTPLFVEMALRVE
                     AIASHTGMKGIFRLLSADVCEVEWVRPVTGFLAPLPEGACADGPTTGPMTEIRALSLV
                     SCRINRAADLESLLEDVLAALDEVFGFRHSMLLLLDETGRSLTTIASRGYGESGVGAE
                     VAVAQGLIGTVAATGAPMRVSGMEALLRYSRAVRGTVEAAGGGAGLRREIPLPGLPHA
                     ESQLALPLLVEERLLGVLALESGDPLAFEEWHESFLLVLANQIAMGIERLSEEAEKEP
                     APPARAGTAAPAAAAHAFLYYEADDCVFVDGEYLIRNVPGRILRKLLRAARDEGRREF
                     TNRELRLDASLGLPTVKDNLETRLILLRRRLEEKCPGVRLVPVARGRFALEMDGAFTL
                     EERRGA"
BASE COUNT          296 a          817 c          898 g          312 t
ORIGIN      
        1 gcgcccatct tcatcggcgt gtgacagtcg tcacagccca tgatgccgac gaggtactgc
       61 ccgcgcgcga cctggggcga ggcgccgaac gtgaggggct tcttcggcgc ctcgcccgcg
      121 tggagagtcg tcgcggcgag gagcgccgcc gcggcgcctg cgaaggcggc tcggcggacg
      181 gtccccggga ccggtcgggc ggacgaaggg cggacggaga gcgtggaaga ggtcatcgga
      241 gtctcccttt cggtcggttg cggcgaaccg ggtttggtac gacggaaggg ggctgaggtt
      301 tcgctcgggc cgttccgccg gggagaaggt gcggcgccgt tcggcgggcg gccacctaag
      361 aactgactaa cgctccctaa gaattcacta atcgcccggc cctgcggtac cctccgcccg
      421 ccgtggccgt tccgggaaag ctcgcgtccc tcctcgaggg gctggtcgcc gttcacgtcg
      481 cgacgcgcga cgccgcgctc gtccccgacg aggtgatggc cgccgccgcc ctcctcgagc
      541 tcgacggacg gagcctgacc gtctaccttc ccgacgcgac ggcggggacg gcgctcgcga
      601 acctgcgtga caacggcgag gtcgcggtcg tcctctcgca gccgctcacg caccggacgg
      661 tgcagctgaa ggggcgcgtg gagacgctgc gcgcggcgcg cgaggaagag cgggaggcgg
      721 tggagcgctt cgccgcggcg ttcgacgcgg aggtggcggc gatcgggctc ccccccgcgg
      781 cgctgcgccg gcggacccgc tggccgtgcg cggcggcgac gttcgcggtg cgcgaagcct
      841 acgagcagac gccgggcccg cgcgccggcg agccgctcgc gccggagggg ccgcggtgat
      901 cgcgaagctc ccggcgctcg cccgctgctt ccaggggatc ctcccgggcg cgctcgcgac
      961 gtgcgcgccc gacgggacgc cgaacgtgac ctacctgagc cagatccgcc aggtggacga
     1021 gcggcacgtc gcgatctcgc gtcagttctt caacaagacg agccggaacc tcgaggcgaa
     1081 cccgcgggcc acggcgacgg tcttcgaccc ggtgacgttc gagcagtgga agctcggcct
     1141 gcgctacgtg cgctccgaga ccgacacgcc cctcttcgtc gagatggcgc tgcgggtcga
     1201 ggcgatcgcc tcgcacacgg ggatgaaggg gatcttccgg ctcctctcgg ccgacgtctg
     1261 cgaggtggag tgggtccgcc cggtgacggg cttcctcgcg ccgcttcccg agggagcctg
     1321 cgcggacggg ccgacgacgg ggccgatgac ggagatccgg gcgctctcgc tcgtctcctg
     1381 caggatcaac cgggcggccg acctcgagtc gctcctcgag gacgtcctcg ccgcgctcga
     1441 cgaggtcttc ggcttccgcc actcgatgct cctgctcctc gacgagacgg gacggagcct
     1501 gacgacgatc gcgagccgcg gctacggcga gagcggcgtc ggcgcggagg tcgcggtcgc
     1561 gcaggggctg atcgggaccg tcgccgcgac gggagcgccg atgcgcgtgt cggggatgga
     1621 ggcgctcctg cgctactcgc gcgcggtccg cgggacggtc gaggcggcgg gcggcggcgc
     1681 ggggctccgg cgcgagatcc cgctcccggg gcttccccac gccgagagcc agctcgcgct
     1741 cccgctcctc gtggaggagc ggctcctcgg cgtcctcgcg ctcgagagcg gagaccctct
     1801 cgcgttcgag gagtggcacg agtcgttcct cctcgtcctg gcgaaccaga tcgcgatggg
     1861 gatcgagcgc ctctcggagg aggcggagaa ggagccggcg ccgccggcgc gggccgggac
     1921 ggcggccccc gcggcggccg cccacgcctt cctctactac gaggccgacg actgcgtctt
     1981 cgtcgacggg gagtacctga tccggaacgt gccggggagg atcctgcgca agctcctgcg
     2041 cgccgcgcgc gacgaggggc ggcgcgagtt cacgaaccgc gagcttcgcc tcgacgcgtc
     2101 gctcggcctg ccgacggtga aggacaacct cgagacgcgg ctgatcctcc tgcgccggcg
     2161 gctcgaggag aagtgccccg gggtgcggct cgtgccggtg gcgcgcggcc gcttcgcgct
     2221 cgagatggac ggcgcgttca ccctcgagga gcgtcgcgga gcctgacggc gccgcgggct
     2281 tccccgcggc cggtcagggg gcgatcgcgt tcggccgggc gag
//
LOCUS       DAOTEZ010000578         2322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_38522_length_2322_cov_5.253639, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000578 DAOTEZ010000000
VERSION     DAOTEZ010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38522_length_2322_cov_5.253639"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1587)
                     /locus_tag="P5164_19620"
     CDS             complement(<1..1587)
                     /locus_tag="P5164_19620"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY46149.1"
                     /translation="MSSQDVDTMKQRPLSPADRRRRLLSSERATETFARGGRHRVALG
                     FPNSYEVGMSNLGFQWVYRLLNREEDVSCERFFYDEAELGGRGPRTLEGEQPLASFPL
                     VAFSVSWEMDYVHFLRMLPAARIPLLREDRGGDDPIVLVGGDCARINPLPLTPYVDAF
                     AMGDGERLVPGIAATLRADLPRRDALERLAALKGFYVPAVHGERAEAAEDGKVVVDQL
                     RAADGPGREPPHTTVLTPHTELSDKLLVEVARGCSEMCRFCWAAYAMAPQVRVPASSI
                     LAVAERCRPLTSRVGLIATAVADHPEILPILRGLSGMGFHIALSSIKIDAISEGLLEV
                     LARQGEKSLAIAPEAGNERLRRAINKKVSDEMLREKVRLVARAGFTNLKLYLQVGLPG
                     ETEEDVDDLVRMVDDLRLVMLEEGRKRGRLGTLVPSVNAFIPKPHTPFETEPLEDPDE
                     LARKLKKLEGAFRAMPNVTFRGMPVAEAIWEAWLAKMGLESGPILAEAAAGAPVRRLV
                     REHRETLLSVARPAARIDAAR"
     gene            1723..2163
                     /locus_tag="P5164_19625"
     CDS             1723..2163
                     /locus_tag="P5164_19625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012833384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HRY46150.1"
                     /translation="MYYVIGADGQQYGPIDEATVRGWLGEGRVNATSLSFKTGEAQWI
                     PLRDRPELGDVLAAPAAVAPPYAPPAGPMPVTPDAPKDWLVTLLLSIFLGSFGVDRFY
                     LGHVGLGIGKLLTFGGCGIWWLIDVILIALGNVTDAKGRPLVKS"
BASE COUNT          330 a          827 c          843 g          322 t
ORIGIN      
        1 ccgggcggcg tcgatgcgcg ccgcggggcg cgcgacggag aggagcgtct cgcggtgctc
       61 ccggacgagc cgtcggacgg gggcgccggc cgccgcctcg gccaggatcg gtcccgactc
      121 gagccccatc ttcgcgagcc aggcctccca gatcgcctcg gcgaccggca tcccgcggaa
      181 cgtcacgttc ggcatcgcgc ggaaggcccc ctcgagcttc ttcagcttcc gggcgagctc
      241 gtcggggtcc tcgagcggct cggtctcgaa cggcgtgtgc ggcttcggga tgaaggcgtt
      301 gacgctcggg acgagcgttc cgaggcgacc gcgcttccgc ccttcctcga gcatcacgag
      361 ccggaggtcg tcgaccatcc ggacgaggtc gtccacgtcc tcctcggtct ctccggggag
      421 gccgacctgg aggtagagct tcaggttcgt gaagccggcc cgcgcgacga ggcgcacctt
      481 ctcgcggagc atctcgtcgg agaccttctt gttgatcgcg cgccggaggc gctcgttccc
      541 ggcctccgga gcgatcgcga gcgacttctc tccctgccgc gcgaggacct cgaggagccc
      601 ctcggagatc gcgtcgatct tgatcgagga gagcgcgatg tgaaagccca tccccgagag
      661 cccccggagg atcgggagga tctcgggatg gtcggcgacg gccgtcgcga tcagtccgac
      721 gcgggaggtg agcgggcggc aacgctcggc gacggcgagg atcgaggagg cgggcacgcg
      781 cacctgaggg gccatcgcgt acgcggccca gcagaagcgg cacatctcgg agcagccgcg
      841 cgcgacctcg acgaggagct tgtcggagag ctcggtgtgg ggcgtcagga ccgtcgtgtg
      901 gggcggctcg cgacccggcc cgtcggccgc ccggagctgg tcgacgacga ccttcccgtc
      961 ctcggccgcc tcggcccgtt cgccgtgcac cgcggggacg tagaagccct tcagcgcggc
     1021 gagccgctcg agcgcgtcgc gtcgcggcag gtcggcccgg agcgtcgccg cgatcccggg
     1081 gacgaggcgc tcgccgtccc ccatcgcgaa ggcgtcgacg tacggcgtca gcgggagcgg
     1141 gttgatccgg gcgcagtcgc cgccgacgag aacgatcggg tcgtcgcctc cgcggtcctc
     1201 ccgcaggagc gggatgcgtg cggcggggag catccggagg aagtggacgt agtccatctc
     1261 ccacgagacg gagaaggcga cgagagggaa ggaggcgagc ggctgctccc cctcgagcgt
     1321 ccgcgggccg cgtccgccga gctcggcctc gtcgtagaag aaacgctcgc aggaaacgtc
     1381 ctcctcccgg ttgaggagcc ggtagaccca ctggaagccg aggttcgaca tcccgacctc
     1441 gtaggagttc gggaagccga gcgcgacacg gtgcctcccg ccgcgcgcga atgtctccgt
     1501 cgctcgctcc gaggagagga ggcggcgtcg gcggtcggcc ggggagaggg ggcgctgctt
     1561 catcgtgtcg acgtcctgcg aggacatctc atgctaaccc ggcgctccga aggccccgcc
     1621 ggacggttcc cggtccccgg cccgctcaca tccgttacga ttcgtcaccc gcgcggaaac
     1681 cttcggggcg cattcgacgt ttcggggagt gagagggacg ccatgtacta cgtcatcggc
     1741 gcagacggac agcagtacgg accgatcgac gaggccaccg tccgcggctg gctcggagag
     1801 gggcgcgtca acgcgacgag cctctcgttc aagaccggcg aggcccagtg gatcccgctg
     1861 cgcgaccgtc ccgagctcgg ggacgtcctc gcggccccgg ccgccgtcgc gccgccctac
     1921 gcgcctcccg ccggcccgat gcccgtgacg cccgacgcgc cgaaggactg gctcgtcacg
     1981 ctcctcctgt cgatcttcct cggctccttc ggcgtcgacc gcttctacct cgggcacgtc
     2041 gggctcggca tcggcaagct cctgacgttc ggcggctgcg gcatctggtg gctcatcgac
     2101 gtcatcctga tcgcgctcgg aaacgtcacc gacgcgaagg gccgcccgct cgtcaagagc
     2161 tgaggggaga cacgaggcgc gcggcgacga actccgccgc gcgctcttcg gcccagtacc
     2221 ggacccggaa cgggctcgcc cccgcgatga aacccgtgtg ccctccgcgg cgcgtccgga
     2281 cgagccggac ggctcccgag gcgcgcgccg cgacccggtc ga
//
LOCUS       DAOTEZ010000579         2309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_38853_length_2309_cov_4.669033, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000579 DAOTEZ010000000
VERSION     DAOTEZ010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38853_length_2309_cov_4.669033"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="P5164_19630"
     CDS             complement(<1..620)
                     /locus_tag="P5164_19630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HRY46151.1"
                     /translation="MTLARTLFTSESVTEGHPDKIADQISDGILDAILADDPEGRVAC
                     ETLVTTGTAFIAGEITTRTYVDIPQVVRETIRDVGYTRAKYGFDYQTCAVLSSIDKQS
                     PDIAMGVDPGGAGDQGLMFGFAVRETDELMPLPITLAHRLTMRLAEARKSHELEWLRP
                     DGKSQVTVEYEGSRPVRVDAVVVSTQHAETVPTLELREAIREEVIRR"
     gene            complement(670..1278)
                     /gene="rsmD"
                     /locus_tag="P5164_19635"
     CDS             complement(670..1278)
                     /gene="rsmD"
                     /locus_tag="P5164_19635"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HRY46152.1"
                     /translation="MIRIVAGEFRGRRLGVGPGVRPTTERAREALFSILGDRVRDARV
                     LDAAAGSGALGFEALSRGAASAVFVEADRRVARVIDANVALVGAGDRAAVVVRTVAGF
                     LRIDRPTGFDLVFFDPPWAEPVASELDGLWRAVSPGGIFVLERGSGGADPWPEAPRTP
                     TTRRYGTTTLYLYSRSLETQADPDGAKSGEDGPRIDLDPAGK"
     gene            complement(1275..2012)
                     /locus_tag="P5164_19640"
     CDS             complement(1275..2012)
                     /locus_tag="P5164_19640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46153.1"
                     /translation="MRRAAAGLVLMSAGLASWGVTPSGEPPVTTAPGPPVVAPTETPR
                     PEDKTLVAALDVEQGDGKDRVSLYRDGTLVLVRTYLGVRTVKKRVLSEVEVDVIRRVA
                     AEALRVDVPEYRIDVLGATKPRRFRIEVGRDGDLPKVFVVDELARVPLALGRTRGALE
                     ELLGRFDESSAEHWQRWDPAAVKAGDVLVRRDDGQRYRVVRDDAFVRSLELLEADRGL
                     QRLVVLREELPKLFLPPGPDASEESRR"
     gene            complement(2009..>2309)
                     /locus_tag="P5164_19645"
     CDS             complement(2009..>2309)
                     /locus_tag="P5164_19645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46154.1"
                     /translation="PLEAPRLFHETLRRALEEVLSEENRGVALTLLLRLDVPAAIVPG
                     WERAHLPEPEELEASSPVPAMWRRLLSPAPDALRARIARRLAFSRALRAASGCGR"
BASE COUNT          337 a          871 c          785 g          316 t
ORIGIN      
        1 cgccggatga cctcctcgcg gatcgcctcg cgcagctcga gcgtcggcac ggtctccgcg
       61 tgctgggtcg acacgacgac ggcgtcgacg cgcaccgggc ggctcccctc gtactccacc
      121 gtgacctggc tcttgccgtc gggccgcagc cactcgagct cgtgcgactt ccgggcctcc
      181 gcgagacgca tcgtgagccg gtgcgcgagc gtgatcggga ggggcatgag ctcgtccgtc
      241 tcgcggaccg cgaatccgaa catcagcccc tggtcccccg cacccccggg gtcgacgccc
      301 atcgcgatgt cgggcgactg cttgtcgatc gacgagagga ccgcgcacgt ctggtagtcg
      361 aaaccgtact tggcgcgggt gtagccgacg tcgcgaatcg tctcccggac gacctgcggg
      421 atatcgacgt acgtgcgcgt cgtgatctcg cccgcgatga acgcggtccc ggtcgtcacc
      481 agcgtctcgc aggcgacccg gccttcggga tcgtcggcca ggatcgcgtc gaggattccg
      541 tcggagatct ggtcggcgat cttgtccggg tgtccctcgg tgaccgactc ggacgtgaaa
      601 agcgtacgcg ccagcgtcat ctcgcggcct cccctgcgtg tcccttcgaa gggcacgaaa
      661 tctgaaagtc tatttcccgg ccggatcgag gtcaattcgc gggccgtcct cccccgactt
      721 cgcgccgtcg ggatcggcct gcgtctcgag cgaccgcgaa tagaggtaga gcgttgtcgt
      781 cccgtagcgc ctcgtcgtcg gggtccgtgg cgcctccggc cacgggtccg cgccgccgct
      841 ccccctctcg aggacgaaga tcccgcccgg agagaccgcg cgccagagtc cgtcgagctc
      901 gctcgcgacc ggctccgccc agggcgggtc gaagaagacg aggtcgaagc cggtcggccg
      961 gtcgattctc aggaaccccg cgacggtgcg gacgaccacg gcggcccggt ccccggcccc
     1021 gacgagcgcc acgttcgcat cgatcaccct tgcgactcgc cggtccgcct ccacgaagac
     1081 cgccgacgcc gctccccggg acagcgcctc gaagccgagc gctccggagc cggccgccgc
     1141 atcgaggacg cgcgcatcgc ggacgcggtc cccgaggatg ctgaagagcg cctcgcgggc
     1201 ccgctccgtc gtcggccgaa cgcccggacc gacgcccagg cgccgccctc ggaactcgcc
     1261 cgcgacgatc cggatcaacg ccggctctcc tcgctcgcgt ccggcccggg cggaaggaac
     1321 agcttcggca gctcctcgcg caggacgacg agccgctgca gaccccgatc ggcttcgagg
     1381 agctcgaggc tccggacgaa cgcatcgtcg cggacgaccc gatagcgctg gccgtcgtcg
     1441 cggcggacga ggacgtcccc ggccttcacg gccgccgggt cccacctctg ccagtgctcc
     1501 gcgctgctct cgtcgaaacg gccgaggagc tcctcgagcg cgccgcgcgt tcggccgagg
     1561 gcgagcggca cgcgcgcgag ctcgtcgacg acgaagacct tcgggaggtc cccgtctcgg
     1621 ccgacctcga tccggaaacg gcgcggtttc gtcgcgccga gcacgtcgat ccgatactcc
     1681 gggacgtcga cccgcagcgc ctcggcagcg acccgacgga tgacgtcgac ctccacctcg
     1741 gaaaggaccc tcttcttcac cgtccggacc ccgagatacg tccggaccag gaccagcgtc
     1801 ccgtcgcggt agagggaaac cctgtccttc ccgtcgccct gctccacgtc gagcgccgcg
     1861 accagggtct tgtcctcggg ccgcggggtc tcggtcggcg ccaccacggg cggacccgga
     1921 gcggtcgtga ccggcggctc tccggagggc gtgacgcccc acgaggccag gcccgccgac
     1981 atgaggacga ggcccgcggc ggcccgtctc atcggccgca ccccgacgcg gcccgaagcg
     2041 cgcgagagaa cgcgaggcgc cgcgcgatgc gggcacgcag cgcatccggc gccggcgaga
     2101 ggagtcgtct ccacatcgcc ggaaccggcg acgacgcctc cagctcttcg ggctcgggga
     2161 ggtgcgctct ttcccagccc ggaacgatcg ccgccggaac gtcgagccga aggaggagag
     2221 tcagcgccac ccctcgattc tcctccgaga gcacttcctc gagcgcgcgg cggagcgtct
     2281 cgtgaaagag acgcggagcc tcgaggggg
//
LOCUS       DAOTEZ010000580         2274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_39835_length_2274_cov_4.300586, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000580 DAOTEZ010000000
VERSION     DAOTEZ010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39835_length_2274_cov_4.300586"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="P5164_19650"
     CDS             <1..497
                     /locus_tag="P5164_19650"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRY46155.1"
                     /translation="GKWSIKEIVAHLADGEVILGSRYRLVAAHEKPAIAAYDQDLFVE
                     RLGADAASAEELVADFEMARAVNLGLLVRLPDEAFARVGIHSERGEESLGTMVAMYAG
                     HDRVHLDQIETIRTGLFPPKKKARKAAPKAAARSAARKPAAAKRAAPKRGAKAPAKKA
                     AKRK"
     gene            complement(507..1655)
                     /locus_tag="P5164_19655"
     CDS             complement(507..1655)
                     /locus_tag="P5164_19655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRY46156.1"
                     /translation="MRRRVPTAGPPLHAVVVGASLAGSAAALVLSRSGGRVALVDRAT
                     FPRSKTCGELLSPDGVAALVRLGLDGAVRAAGAATIRRFELARPDGSRVAGRLPGPAL
                     SLSRERLDLLVLDGARVAGATAHLGETVVSIDGTLPKGFVVRTTARTLEARTVLGAWG
                     RYSALDGRLGRPFFGSPASLFGFKRFLAGGAGRRFEDRAVLHVFRGGYLGLSLVEEGR
                     VSLGALATPEVAKEAHHDLDRLLARLGKECPGLAADLEGLAPEPGPTLVSEPVHLGRR
                     GAVLGDVLLAGDAAGVVDPYTGSGMALALRTGEAAGSLLAELAAGRVGAEALARSWER
                     RWRKVTGSVYFFSRLFRPVFLGGFATRLVVPATAPLAGLAARLTRGAA"
     gene            1777..>2274
                     /locus_tag="P5164_19660"
     CDS             1777..>2274
                     /locus_tag="P5164_19660"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HRY46157.1"
                     /translation="MSDSRFEFRGDLSKTPLPEVLQTVHHYRVPGVLVVRRGEVEKKI
                     YLWNGDVIFATSSDREESLGNVLLRAGVLTREQFDESVKRLVEARAAEETRRHGAVLV
                     DMGLLTQEALTESVMRQVRDILYSVFDWEEGSVTFAVGHFRTDEIIQLEIPTRQAIVA
                     GVKAVR"
BASE COUNT          340 a          819 c          849 g          266 t
ORIGIN      
        1 cggggaagtg gtcgatcaag gagatcgtcg ctcacctggc cgacggcgag gtgatcctcg
       61 gctctcgcta ccgcctcgtc gcggctcacg agaagcccgc gatcgcggcc tacgaccagg
      121 acctcttcgt cgagcgcctg ggcgcggacg ccgcttccgc cgaggagctc gtcgccgact
      181 tcgagatggc ccgcgcggtg aacctcgggc tcctcgttcg gctgccggac gaggcgttcg
      241 cacgggtcgg gatccactcc gagaggggcg aggagtctct cggcaccatg gtcgcgatgt
      301 acgccgggca cgaccgcgtc cacctcgacc agatcgagac gatccggacc ggcctcttcc
      361 cgccgaagaa gaaggcgcgg aaggccgccc cgaaggcggc cgccaggagc gcggcgcgta
      421 agccggcggc cgcgaagcgg gccgcgccga agcgcggagc gaaggccccc gcgaagaagg
      481 ccgcgaagag gaagtaggcg gctccgtcag gcggcgccgc gcgtgaggcg ggccgcgagg
      541 cccgccagcg gagccgttgc cgggacgacg aggcgggtgg cgaacccccc gaggaagacg
      601 gggcggaaga gccgcgagaa gaagtagacg ctcccggtca ccttccgcca ccgtcgctcc
      661 cagctccgcg cgagcgcctc cgccccgacg cgtcctgccg cgagctcggc caggagagaa
      721 cctgcggcct cgccggtccg gagcgcgagg gccattccgg acccggtgta cgggtcgacg
      781 acgcccgcag cgtcgccggc gaggaggacg tcgccgagga ccgcccccct ccggccgagg
      841 tggaccggct cgctgacgag ggtcggaccg ggctccggcg cgaggccctc gaggtcggcg
      901 gcgaggcccg ggcactcctt cccgaggcgc gcgaggagcc ggtcgaggtc gtgatgcgcc
      961 tccttcgcga cctccggcgt cgcgagagct ccgaggctga ctcgcccctc ctcgacgagg
     1021 gagaggccga ggtagccgcc gcggaagacg tgcaggacgg cccggtcctc gaaccgcctc
     1081 cccgcccccc cggcgaggaa ccgcttgaag ccgaagagcg aggccggcga cccgaagaac
     1141 ggccttccga gacgtccgtc gagcgccgag tagcgccccc aggcgccgag gaccgtacgc
     1201 gcctcgagtg tgcgggccgt cgttcggacg acgaatccct tcgggagcgt cccgtcgatc
     1261 gagacgacgg tctcgccgag gtgcgccgtc gccccggcca cccgggcgcc gtcgaggacg
     1321 aggaggtcga ggcgctcccg gctcagcgaa agggccggtc cgggaaggcg tcccgcgacg
     1381 cggcttccgt cggggcgcgc gagctcgaag cggcggatgg tcgcggcccc cgcggcgcgg
     1441 accgcgccgt cgagcccgag ccgcacgagg gctgcgacgc cgtccgggga gaggagctcc
     1501 ccgcaggtct tcgagcgggg gaaggtcgcc ctgtcgacga gggcgacgcg cccgcccgag
     1561 cgggagagga cgagcgcggc ggcgctcccc gcgaggctcg cgccgacgac gacggcatga
     1621 agcggcgggc cggccgtcgg gacgcggcgc ctcatcgggc cccgttcccg agccgtcgtc
     1681 ctcccccttc cggagcggcc gccgtctgcc gtcggcagat caaacgccgg catcgtagca
     1741 gccggtccgg ggccagagtt ccgtacaatc cggggggtgt cggacagccg cttcgagttc
     1801 cgcggcgacc tctcgaaaac cccgcttccc gaggtcctcc agacggtgca ccactaccgt
     1861 gtgcccggcg tgctggtcgt gcgccggggc gaggtggaga agaagatcta cctctggaac
     1921 ggcgacgtca tcttcgccac ctccagcgac cgggaagagt ccctcggcaa cgtcctcctc
     1981 cgggccgggg tgctcacgcg ggagcagttc gacgagtcgg tgaagcggct cgtcgaggcg
     2041 cgcgccgccg aggagacccg ccggcacggg gcggtcctgg tcgacatggg cctcctgacg
     2101 caggaagcgc tcaccgagtc cgtcatgcga caggtccgag acatcctcta ctccgtcttc
     2161 gactgggagg aggggtcggt cactttcgcc gtcggacact tccgcaccga cgagatcatc
     2221 cagctggaga tcccgacgcg tcaggcgatc gtcgcgggcg tcaaggcggt tcgc
//
LOCUS       DAOTEZ010000581         2274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_39837_length_2274_cov_3.517801, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000581 DAOTEZ010000000
VERSION     DAOTEZ010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39837_length_2274_cov_3.517801"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="P5164_19665"
     CDS             <1..1184
                     /locus_tag="P5164_19665"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRY46158.1"
                     /translation="GKPVTLTRDVALEEWKRFRDEVMTGPAAPPPEPLTFGRYVELHW
                     KDIIRSASERTRRGYAVHMNHIILPELGPMLLEKVNPATLRDLAGRMKDGENVVATGK
                     PKAGGYSANTINHCAVLVRRVLLDAVDRGDLPRYPFLRREKKEKPVLLKLELNPEERE
                     AFLASFDDEQGFRRLWTARRDADAKRRAEVPGRPGGPWEPTGEAVAFHFERLRASYPF
                     FAVALETGLRRGDLLELRWTSIDLREGVVIVQMRKTKEEAVIPMSGRLRDALNVLRFG
                     AADDGYVFESEVEIDGQRRRQRWSWTVLERYFRLAKTIAGITRRFRFHDIRHSFCSTM
                     VSAGVPLEVLQKMTGHTTLQMLQRYARPDLATLRRAMATAEMSRGMSIKPVRMDRGAA
                     S"
     gene            1492..2193
                     /locus_tag="P5164_19670"
     CDS             1492..2193
                     /locus_tag="P5164_19670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46159.1"
                     /translation="MARPRKDSFYALAEVMRQNPKDVLKTAGAIQALYLLPKETKPED
                     VAAVVRRIQRDKVPRLMAALLRGAAEQVLAPKRKGGRPRKQPDPFDALLLLRQRRELD
                     LVAGAIEGGDEKEMKKVANSFPAKRSRPVALKAMARYRAEIDEAEDDEKMTGRLRVYA
                     ARVILEARGGAAATEAEGSALLRRVQRGGALRTSAGIVKTWGLSLGREESAEGLLAAL
                     ERADASREKEVPRKR"
BASE COUNT          404 a          740 c          804 g          326 t
ORIGIN      
        1 acgggaagcc cgtcaccctc acccgcgacg tggccctcga ggagtggaag cgcttccgcg
       61 acgaggtgat gaccgggccg gccgcgcctc cgcccgagcc cctcacgttc ggccgctacg
      121 tcgagctcca ctggaaggac atcatccgtt cggcgagcga gcggacccgg cgcgggtacg
      181 ccgtccacat gaaccacatc atcctgcccg agctcgggcc gatgctcctc gagaaggtga
      241 acccggcgac gctccgcgac ctcgcggggc ggatgaagga cggcgaaaac gtcgtcgcca
      301 ccggcaagcc gaaggcgggg ggctactccg cgaacaccat caaccactgc gccgtgctcg
      361 tccgtcgggt cctcctcgac gccgtcgacc gcggcgacct cccgcgctac ccgttcctcc
      421 gccgcgagaa gaaggagaag ccggtcctcc tgaagctcga gctgaacccg gaggagcgcg
      481 aggcgttcct cgcgtcgttc gacgacgagc agggcttccg gcgcctctgg acggcccgta
      541 gggacgccga cgcgaagagg cgggccgagg tcccgggtcg ccctggcggg ccctgggagc
      601 cgactggcga ggccgtggcg ttccacttcg agcgcctccg cgcgtcctac ccgttcttcg
      661 ccgtggccct cgagacgggg ctccgccgcg gcgacctcct cgagctccgc tggacgtcga
      721 tcgacctccg cgaaggggtc gtgatcgtcc agatgcggaa gacgaaggag gaggcggtca
      781 tcccgatgtc ggggcggctg cgcgacgcac tgaacgtcct acgctttggc gcggccgacg
      841 acggctacgt cttcgagagc gaggtcgaga tcgacgggca gcgccgccgg cagcgctggt
      901 cgtggacggt cctcgaacgc tacttccggc tcgcgaaaac gatcgcgggt atcacccgga
      961 ggttccggtt ccacgatatc cggcatagct tctgttcgac gatggtgtcg gccggcgtgc
     1021 cgctcgaggt tctgcagaag atgaccggcc acacgacctt gcagatgctc cagcgctacg
     1081 cgcggcccga cctcgcgacg ctgcgtcgcg cgatggcgac cgccgaaatg tcgaggggaa
     1141 tgtcaatcaa gccggttcgg atggataggg gagcggcctc ttgaggccgg aagtgattgg
     1201 ctcgccgtcg gataagtggt gggcccgggg agactcgaac tcccgacaca cggtttagga
     1261 acgaagttat tgactatcaa cgagttagaa tcaatgagtt acgagggttc taatgcgttc
     1321 taactcgcag tttagtgggt cgcggctccc gaatgtcaac ggaatgtcaa cgctaacgta
     1381 ggacgatagc cgtgccgggc cgccacccgt gggagtagct acccacgggc gcgacctccg
     1441 ccgcaaggcg gcccggccct ttcccctggg gcggaggaga gcggaggacc gatggcgaga
     1501 ccgaggaagg acagcttcta cgccctggcc gaggtcatgc ggcagaaccc gaaggacgta
     1561 ctgaagacgg cgggcgcgat ccaggcgctc tatctcctgc cgaaggagac gaagcccgaa
     1621 gacgtcgcgg cggtcgtccg gagaatccag cgagacaagg tcccgcgtct catggccgcc
     1681 cttcttcgcg gggccgccga gcaggtgctc gcgccgaaac ggaaaggggg ccggccgaga
     1741 aagcagcccg accccttcga cgctctcctt ctcctccggc agcgtcgcga gctcgacctc
     1801 gtggccggcg cgattgaggg cggcgacgag aaggagatga agaaggtcgc gaacagcttc
     1861 ccggcgaagc ggagtcgccc cgtcgctctc aaggcgatgg cgcggtacag ggcagagatc
     1921 gacgaggccg aggacgacga gaagatgacc ggccgtcttc gcgtctacgc cgctcgcgtg
     1981 atcctcgagg cccgcggcgg cgccgcggcg accgaagcgg aagggagcgc actcctgcgg
     2041 cgtgtccaac gtggcggtgc cctccgtacg tcggcgggga tcgtcaagac gtggggcctc
     2101 tcgttggggc gggaggagag cgcggaaggt ctgctcgcgg cgctggaaag ggcagatgca
     2161 agccgcgaga aagaggtccc ccggaagcgg tagaaacccc ctcccataaa cccgggtttc
     2221 gtggagcgcc gacgaggcgc ttctgacggg cgcggcgggg agcttcggga caca
//
LOCUS       DAOTEZ010000582         2260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_40225_length_2260_cov_6.294785, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000582 DAOTEZ010000000
VERSION     DAOTEZ010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40225_length_2260_cov_6.294785"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1010)
                     /gene="hemH"
                     /locus_tag="P5164_19675"
     CDS             complement(12..1010)
                     /gene="hemH"
                     /locus_tag="P5164_19675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HRY46160.1"
                     /translation="MTAGKTGILLVQLGGPTKREELKGFLYRLFADPEILGIPFAPLR
                     KAIAWTIATTREAESAKTYEKIGWSPIRCWTERSAMLLEAALAPSWPGEPPIVRSAMT
                     CSAPLVEEVLPSLRAAGVTRLFVLPLYPQYSVTTTKGSFARVDEALSRMGWSPERVNA
                     PDSWYAEPRFLDAHADRILAAAALLPDPEPAATVLLYSAHSLPVSTVEKKKDPYPKQI
                     EECCALVDERLGRRFRSRLGYQSKLGPVAWLGPSTGEVLAELAREGVKQVVVCPIAFV
                     SDHVETLYEIRMLFAEEARAAGITHYVAADGLNDHPAFVDALADVCRKALLAPGAR"
     gene            complement(1010..>2260)
                     /gene="hemN"
                     /locus_tag="P5164_19680"
     CDS             complement(1010..>2260)
                     /gene="hemN"
                     /locus_tag="P5164_19680"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HRY46161.1"
                     /translation="PISLYVHLPFCDEQCWFCGCFMKVVPKPDRQGEERGEVEGYLAD
                     LHREIDLLAARVDKGRPVVQVHWGGGTPTYLTPKQADALAEHLLAAFRPAPGAEISVE
                     IDPRVTTPEHLAVLRRHGWNRVSMGVQDFDPEVQELVNRVQPFEMTKALVDASRELGY
                     ESLNLDLIYGLPGQKLAGFSRSVEQVLTLRPDRIAMYSYAHVPWLKKPQRVLAAHLPE
                     GTEKFAIFVSGIEKFSEAGYVYIGMDHFALPHDEIARAQVERTLWRNFQGYTTHAGVD
                     LYALGVSAIAQIDDCYVQNTKDYAAYHAACAEGRPSTIKGWRLTEEDHLRRTVITNLL
                     CHCVIDKREIARQFGLASFDETFAREVAALPAFVADGLVEPRTDEIRVTPLGRIFIRN
                     VAMLFDPYLLKRPADQPKVFSRTL"
BASE COUNT          336 a          756 c          832 g          336 t
ORIGIN      
        1 gacgatccgc ctcaacgagc tcccggcgcg aggagcgcct tgcggcagac gtcggcgagc
       61 gcgtcgacga acgcggggtg gtcgttcagc ccgtccgcgg cgacgtagtg cgtgattccg
      121 gccgccctcg cctcctcggc gaagagcatc cggatctcgt agagcgtctc gacgtggtcg
      181 gagacgaagg cgatcgggca gacgacgacc tgcttcaccc cctcgcgcgc gagctcggcc
      241 aggacctccc cggtggacgg tccgagccag gcgaccgggc cgagcttcga ctggtagccg
      301 aggcgcgagc ggaagcgccg cccgaggcgt tcgtcgacga gagcgcagca ctcctcgatc
      361 tgcttcgggt aggggtcctt cttcttctcc accgtggaga cggggaggga atgggccgag
      421 tagaggagga ccgtcgccgc cggctcggga tcggggagca gggccgccgc cgcgaggatc
      481 cggtccgcgt gcgcgtcgag gaacctcggc tcggcgtacc aggagtccgg cgcgttcacc
      541 ctctcgggcg accaccccat ccgcgagagc gcctcgtcga cacgcgcaaa ggagcccttc
      601 gtcgtcgtca cggagtactg cgggtagagc gggaggacga agaggcgcgt cacgcccgcg
      661 gcgcgcaggg agggcagcac ctcctcgacg agcggggcgc tgcacgtcat cgcggagcgg
      721 acgatcggcg gctcgccggg ccaggagggg gcgagcgccg cctcgaggag catcgccgac
      781 ctctccgtcc agcaccggat cggcgaccag ccgatcttct cgtaggtctt cgccgactcg
      841 gcttcgcgcg tcgtcgcgat cgtccaggcg atcgccttcc ggagcggcgc gaaggggatg
      901 ccgaggatct ccgggtcggc gaagagccgg tagaggaagc ccttcagctc ctcccgcttc
      961 gtcggaccgc cgagctggac caggaggatc ccggtctttc cggcggtcat cagagcgtcc
     1021 tggagaagac cttcggctgg tcggccggac gcttcaggag gtacgggtcg aagagcatcg
     1081 ccacgttccg gatgaagatc cggccgagcg gcgtcacgcg gatctcgtcc gtccgcggct
     1141 cgacgagccc gtccgcgacg aacgcgggga gggccgcgac ctcgcgggcg aacgtctcgt
     1201 cgaaggaggc gaggccgaac tgccgggcga tctcccgttt gtcgatgacg cagtggcaga
     1261 ggaggttcgt gatgaccgtc cgccggaggt ggtcctcctc cgtcagccgc caccctttga
     1321 tcgtcgaggg gcgcccctcg gcgcaggcgg cgtggtaggc ggcatagtcc ttcgtgttct
     1381 ggacgtagca gtcgtcgatc tgggcgatcg ccgagacgcc gagggcgtag aggtcgacgc
     1441 ccgcgtgcgt cgtgtagccc tggaagttcc gccagagggt ccgctccacc tgcgcgcgcg
     1501 cgatctcgtc gtgcggcagg gcgaagtggt ccatcccgat gtagacgtag cccgcctcgg
     1561 agaacttctc gatccccgag acgaagatcg cgaacttctc cgtcccttcc ggcaggtgcg
     1621 ccgcgaggac gcgctgcggc ttcttcagcc acgggacgtg ggcgtaggag tacatcgcga
     1681 tccggtcggg ccggagcgtc agcacctgct cgacgcttcg cgagaagccg gcgagcttct
     1741 ggcccggcag gccgtagatg aggtcgaggt tgagcgactc gtagccgagc tcgcgcgagg
     1801 cgtcgacgag cgccttcgtc atctcgaacg gctggacgcg gttgacgagc tcctggacct
     1861 cggggtcgaa gtcctggacg cccatcgaga cccggttcca gccgtgccgg cggaggaccg
     1921 cgaggtgctc gggcgtcgtg acgcgcgggt cgatctcgac cgagatctcc gccccgggcg
     1981 ccggccggaa cgccgcgagg aggtgctccg cgagggcgtc ggcctgcttc ggcgtcaggt
     2041 aggtcggcgt cccgcctccc cagtggacct ggacgacggg gcggcccttg tcgacccgcg
     2101 cggcgaggag gtcgatctcg cggtggaggt cggcgaggta cccctcgacc tccccccgct
     2161 cctccccctg ccggtcgggc ttcgggacga ccttcatgaa gcagccgcag aaccagcact
     2221 gctcgtcgca gaacgggagg tggacgtaga gcgagatcgg
//
LOCUS       DAOTEZ010000583         2248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_40558_length_2248_cov_4.781578, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000583 DAOTEZ010000000
VERSION     DAOTEZ010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40558_length_2248_cov_4.781578"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..856)
                     /gene="surE"
                     /locus_tag="P5164_19685"
     CDS             complement(80..856)
                     /gene="surE"
                     /locus_tag="P5164_19685"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRY46162.1"
                     /translation="MRALVSNDDGIHSPGIEALERAAKAAGFETWVVAPDREQSATSH
                     ALTLTRPLRVVKTGERSWVVDGTPTDCVNLAICRILKDVRPDFVFSGINLGPNLGDDV
                     TYSGTVAAAFEGTLLGVPSIAFSLDCRKDGGAGPDFSEAEKVAEEVIRLALSRPMAED
                     VLWNVNVPEGRPTGIRATRMGRRRYGESVVEKVDPRGRPYYWIGGSHVDTHSDGSDLT
                     TVAEGLVSVTPLHLDLTAYRSLEDLAARLAGSRANGPDRS"
     gene            complement(856..1293)
                     /locus_tag="P5164_19690"
     CDS             complement(856..1293)
                     /locus_tag="P5164_19690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRY46163.1"
                     /translation="MTETTAAQPLTRITTTATFSAAHRLHNPSRDDEWNRVTFGKCNN
                     PHGHGHTYTVEVTVEGTIDPVTGWVLDFSHLKRIVDEKVVRRCDHRNLNVDVDFLAGV
                     VPTAENLAVCFWNELAPHVAPARLVKLSLHETERNRVVYRGGA"
     gene            complement(1393..>2248)
                     /locus_tag="P5164_19695"
     CDS             complement(1393..>2248)
                     /locus_tag="P5164_19695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46164.1"
                     /translation="IPQSEVGSPFRPDVFVVARDFQNPRTVAASVGIEREVMKDYGVL
                     VKYNYAKTTHLTRFINRNDALLGSPWSSGLNGGPNGIGTLTVVESSARSLYQGVTFGV
                     SKRWSDDYQFQLNYTLSWDKSDDDNERDPFSFRYAKVTDLDAEYGYSDRDQRHRLNGV
                     FVWKAPGDVNVNLRYSYRSAQPKSITRTGADANTPQERINPDGTVTQRNLGRKDNQFS
                     SLDLRLSRSFELGPVQVEPILECFNVFNSKNLKRPEVTNLIFNFDGTVQSGLGDPRQV
                     QLGLRVVF"
BASE COUNT          309 a          764 c          802 g          373 t
ORIGIN      
        1 caaccatggc gcgccgcgcc tcggcgcccg cccgcgcggc gcgcgcgacc tcgggatcct
       61 ccggcctcac gccgggatct tagcttcggt ccggcccgtt cgcgcggctc cccgcgagcc
      121 gcgcggcgag gtcctcgagc gacctgtagg ccgtgaggtc gaggtgaagc ggcgtcaccg
      181 acacgagccc ctcggcgacg gtcgtcaggt cgctcccgtc ggagtgggtg tcgacgtggg
      241 agccgccgat ccagtagtag ggcctcccgc gggggtccac cttctccacg acgctttcgc
      301 cgtagcggcg cctccccatg cgggtcgccc ggatccccgt cggccgcccc tccgggacgt
      361 tcacgttcca gaggacgtcc tccgccatcg ggcgggagag cgccagccgg atgacctcct
      421 ccgcgacctt ctccgcctcc gagaaatcgg ggcccgcgcc gccgtccttc cggcagtcga
      481 gggagaacgc gatggacgga acgccgagaa gggtcccctc gaaggccgcc gcgaccgttc
      541 ccgaataggt gacgtcgtcg ccgaggttcg gtccgaggtt gatcccggag aagacgaagt
      601 ccggccggac gtccttcagg atccggcaga tcgcgaggtt cacgcagtcc gtcggcgtcc
      661 cgtcgacgac ccacgagcgc tcccccgtct tcaccacccg caggggccgg gtgagcgtca
      721 gcgcgtggga ggtcgcgctc tgctcccgat cgggggcgac gacccaggtc tcgaagccgg
      781 cggccttcgc cgcccgctcg agcgcctcga ttcccgggct gtggatcccg tcgtcgttcg
      841 agacgagggc gcgcatcagg cgccgccccg gtagacgacc cggttccgct ccgtctcgtg
      901 gagggagagc ttcacgaggc gcgccggggc gacgtgcggg gcgagctcgt tccagaagca
      961 gacggcgagg ttctcggccg tcgggacgac ccccgcgagg aagtccacgt cgacgttcag
     1021 gttccggtgg tcgcagcgcc ggacgacctt ctcgtcgacg atccgcttca ggtgcgaaaa
     1081 gtcgaggacc cacccggtga ccgggtcgat cgtcccctcg acggtcacct ccacggtgta
     1141 ggtatggccg tgcccgtgag ggttgttgca cttgccgaac gtgacgcggt tccactcgtc
     1201 gtcccgcgag gggttgtgga ggcggtgcgc cgcgctgaac gtggcggtcg tggtgatccg
     1261 ggtgagcggc tgggccgccg tcgtctctgt cacggtaaag gctccggccc cgggaggggc
     1321 gagggcgccc gtcgattccg ccgggcgccc gtcccggaat ctagcacgcg tcgatcgctc
     1381 ctcccggacg tctcagaaga cgactcggag gccgagctgg acctgcctcg ggtcgccgag
     1441 gccgctctgg accgtcccgt cgaagttgaa gatcaggttc gtcacctcgg gcctcttgag
     1501 gttcttcgag ttgaagacgt tgaagcactc gaggatcggc tcgacctgga ccggcccgag
     1561 ctcgaaggag cgcgagagcc gcaggtcgag cgaggagaac tggttgtcct ttcgcccgag
     1621 gttccgctgg gtgacggtgc cgtcggggtt gatcctctcc tgcggcgtgt tcgcgtcggc
     1681 gccggtccgg gtgatcgact tcggctgcgc cgaccggtag gagtagcgga ggttgacgtt
     1741 cacgtcgccg ggcgccttcc agacgaacac gccgttcagg cggtggcgct ggtcgcggtc
     1801 ggagtagccg tactcggcgt cgaggtcggt caccttcgcg tagcggaacg agaacgggtc
     1861 ccgctcgttg tcgtcgtccg acttgtccca cgagagggtg tagttcagct ggaactggta
     1921 gtcgtccgac caccgcttgc tcacgccgaa cgtgaccccc tggtagaggc tccgtgcgga
     1981 cgactcgacg accgtcagcg tcccgatgcc gttcggcccg ccgttcaggc ccgaggacca
     2041 gggcgagccg aggagcgcgt cgttgcggtt gatgaagcgg gtcaggtggg tcgtcttggc
     2101 gtagttgtac ttgacgagga ccccgtagtc cttcatcacc tcgcgctcga tgccgacgct
     2161 cgccgcgacc gtccgcgggt tctggaagtc cctcgcgacg acgaagacgt cgggccggaa
     2221 cggggagccg acctccgact gcgggatg
//
LOCUS       DAOTEZ010000584         2245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_40653_length_2245_cov_5.652968, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000584 DAOTEZ010000000
VERSION     DAOTEZ010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40653_length_2245_cov_5.652968"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2245
                     /locus_tag="P5164_19700"
     CDS             <1..>2245
                     /locus_tag="P5164_19700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005038009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HRY46165.1"
                     /translation="AAGTSLSGQAVTDGLLVEVARHWRTVKVEDGGKRFRAQPGVIGA
                     SVNNALRPYRVKMGPDPASINTCTMGGILSNNSSGMCCGVTQNAYHTLESLTFLLPSG
                     TRIDTAAPDADARFRAAEPALWQGLLDLKATLEADPALVARIRSKYRIKNTTGYSLNA
                     FLDFERPVEIFRNLLVGAEGTLAFISEAVLSTVPDLPVKYTGLLLFPSMHAACAAIVP
                     LRDSGAKALELLDRASLRSVETQPGVPPSIRTLPEGAAALLVEYQAGAEAERTGLEAV
                     ASAAVAPLALLEPARFTHDATEQALLWKVRQGTFPSVGAARKSGTTVLIEDVAFPIEH
                     LADAAVDLTRLFAKHGYPEAILFGHAKDGNLHFVITQSFNDPAAVEKYARLIDDVVEL
                     VVKRYDGALKAEHGTGRNMAPFVETEWGPEAYAVMKRLKALCDPEGILNPGVILNGDP
                     LCHLKDVKPMPTVEEEVDKCIECGYCEPKCPSRELTLTPRQRIVLRREMTRLEAEGTN
                     APLLAALEAEFPYAAVDTCAVDGLCATACPVSIDTGQLTKRLRKARHSARARKAALSV
                     ARRFATVEPLLRTGLRAGHLVQSVLGAGAMAGITRAIRAVVGKPFPQWSAEMPRAAKA
                     SRPATTKEGASAVYFPACISRTMGALPGEPSETSLMEAFVAVAQRAGTPVFIPEDVEG
                     VCCGVPFSSKGYTEANAFAANRAIERFWAWSDGGRLPVVVDTSPCTYGVKGSRPSLTP
                     ENQARFDR"
BASE COUNT          328 a          840 c          778 g          299 t
ORIGIN      
        1 gcggcgggaa cctcgctctc ggggcaggcc gtcacggacg gcctcctcgt cgaggtcgcc
       61 cgccactggc ggaccgtgaa ggtcgaggac ggcgggaaga gattccgggc gcagcccggc
      121 gtcatcggcg cctccgtgaa caacgcgctc aggccctacc gcgtcaagat ggggcccgac
      181 cccgcctcca tcaacacgtg cacgatgggc gggatcctct cgaacaactc gagcggcatg
      241 tgctgcggcg tgacgcagaa cgcctaccac acgctcgagt cgctcacgtt cctcctcccc
      301 tcggggacgc ggatcgacac ggcggccccc gacgccgacg cgcgcttccg cgcggccgag
      361 ccggccctct ggcaggggct cctcgacctc aaggcgacgc tcgaggcgga cccggctctc
      421 gtcgcccgca tccggtcgaa gtaccggatc aagaacacga ccggctactc gctgaacgcc
      481 ttcctcgatt tcgagaggcc cgtcgagatc ttccggaacc tcctcgtggg ggcggaaggg
      541 acgctcgcgt tcatctccga ggcggtcctc tccacggtcc cggacctccc ggtcaagtac
      601 accggcctcc tcctcttccc ctcgatgcac gcggcctgcg cggcgatcgt cccgctccgc
      661 gactcgggcg cgaaggcgct cgagctcctc gaccgcgcct cgctccgttc cgtggagacg
      721 cagccggggg tcccgccctc catccggacg cttcccgaag gcgccgcggc gctcctcgtc
      781 gagtaccagg cgggggccga ggccgagcgg acggggctcg aggccgtcgc gtccgcggcg
      841 gtcgcgccgc tcgccctcct cgagccggcc cgcttcacgc acgacgccac ggagcaggcg
      901 ctcctctgga aggtgaggca ggggacgttc ccgtccgtcg gcgccgcccg gaagagcggg
      961 acgaccgtcc tcatcgagga cgtcgcgttc ccgatcgagc acctcgccga cgccgccgtc
     1021 gacctgacgc ggctcttcgc gaagcacggc tacccggagg cgatcctctt cggccacgcg
     1081 aaggacggga acctccactt cgtcatcacg cagtcgttca acgacccggc ggccgtcgag
     1141 aagtacgcgc ggctcatcga cgacgtcgtc gagctcgtcg tgaagaggta cgacggggcg
     1201 ctgaaggcgg agcacggcac gggccggaac atggcgccgt tcgtcgagac ggagtggggc
     1261 ccggaggcct acgccgtcat gaagcggctg aaggcgctct gcgaccccga gggcatcctg
     1321 aacccgggcg tcatcctgaa cggcgacccg ctctgccacc tgaaggacgt caaaccgatg
     1381 ccgaccgtcg aggaagaggt cgacaagtgc atcgagtgcg gctactgcga gccgaagtgc
     1441 ccgagccgcg agctgacgct cactccccgc cagcggatcg tcctccgccg cgagatgacc
     1501 cgcctcgagg cggaggggac gaacgcgccg ctcctggcgg ccctcgaggc cgagttcccg
     1561 tacgccgcgg tcgacacctg cgcggtcgac ggactctgcg cgacggcctg cccggtctcg
     1621 atcgacacgg gccagctgac gaagcgactc cggaaggcgc gccactcggc gcgcgcccga
     1681 aaggccgcgc tctccgtcgc ccgccgcttc gcgacggtcg agccgctcct ccgaaccggc
     1741 ctccgcgccg ggcacctcgt ccagtcggtg ctcggggcgg gcgcgatggc ggggatcacg
     1801 cgcgcgatcc gggcggtcgt cgggaagccc ttcccgcaat ggagcgccga gatgccgcgg
     1861 gccgcgaagg cctcgcgccc ggcgacgacg aaggagggcg cctccgcggt ctacttcccg
     1921 gcctgcatct cgcggacgat gggggcgctc cccggcgagc cgtcggagac gtcgctcatg
     1981 gaggcgttcg tcgcggtcgc gcagcgggcc gggacgccgg tcttcatccc ggaggacgtc
     2041 gaaggcgtct gctgcggcgt ccccttctcc tcgaaggggt acacggaggc gaacgcgttc
     2101 gccgcgaacc gggcgatcga gcgtttctgg gcctggtccg acggcgggcg cctgccggtc
     2161 gtcgtcgaca cgagcccctg cacctacggc gtgaagggga gccgcccgag cctcacgccc
     2221 gagaaccagg cgcgcttcga ccggc
//
LOCUS       DAOTEZ010000585         2236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_40913_length_2236_cov_5.636864, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000585 DAOTEZ010000000
VERSION     DAOTEZ010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40913_length_2236_cov_5.636864"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="P5164_19705"
     CDS             <1..544
                     /locus_tag="P5164_19705"
                     /inference="COORDINATES: protein motif:HMM:NF017733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HRY46166.1"
                     /translation="RDLPSRLPFEGGEADFQLEGAGVFSGIRCLRRPTPTVRPPLRRG
                     LQWRLLSHLSLNLLSLVERAEGAGPEALQEILRLYDFADSSVTRQQIAGITKVGARRV
                     LRSIGVLQPSFVRGVEVSIELDENQFVGTGIFLFAQVLERFLGLYSSVNSFSQLVLSS
                     RQREGVVRRWEPRAGEQIVL"
     gene            551..1546
                     /gene="tssG"
                     /locus_tag="P5164_19710"
     CDS             551..1546
                     /gene="tssG"
                     /locus_tag="P5164_19710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HRY46167.1"
                     /translation="MKDLLFAEPYRFSFFQAVRLLERTSPGKEGVGRAVRPGNEVVRF
                     RSHPSLTFPASEIADLKAPPPGAEDRPPEMTVAFIGLTGPLGILPHPYTELVRERASY
                     KDTALWSFLDLFHHRFTSLFYRAWEKYRFPIAFERSGEDSFTEYVFDLIGMGTPGLRG
                     RMTVRDQALLLYAGLIAQKPHSGESIAAVLRDYFGVPAEIVQFQGQWFPMEEENVTRL
                     GAANSELGRTVVAGSSVFVSQSKFRVRLGPLPLSAFVSFLPVGPAFRPLTELVRFLAG
                     LEFDYDVQLVLRKEEVPPCSLDSSAALPPMLGWTTWITTETPREDAGDVVLPVEN"
     gene            1581..>2236
                     /locus_tag="P5164_19715"
     CDS             1581..>2236
                     /locus_tag="P5164_19715"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HRY46168.1"
                     /translation="MNLKSLIGRLNDTCRQALEGAAGLCLSRTNYDVEVEHLLLKLLE
                     APDTDLAKILRRFEIDASRFAKDVVRALDRLKTGNARTPALSPRLPRLIEKAWVVASI
                     EYGASRVRSGHLVHVLLTDDDLSRLAREISKDLAGISVEQLTKTFPDVVKGSAEDREA
                     QALSEAPGAGGGPDVAMGVPGKTKSLDQYTEDLTAKARAGKIDPILGRDFEIRQVIDI
                     L"
BASE COUNT          306 a          836 c          737 g          357 t
ORIGIN      
        1 ccgggacctc ccctcgcgcc tcccgttcga agggggggag gccgacttcc agctggaggg
       61 ggccggggtc ttctcgggca tccgctgcct gcgtcgaccg acgccgaccg tgcgtccgcc
      121 gctccggagg gggctccagt ggcggcttct ctcgcacctc tcgctgaacc tcctctccct
      181 cgtcgagcgg gcggaaggcg cggggcccga ggcgctgcag gagatcctcc ggctctacga
      241 cttcgccgac tcgtccgtca cgcggcagca gatcgccggg atcacgaagg tcggcgcccg
      301 gcgcgtcctc cgctcgatcg gggtcctgca gccgagcttc gtccgcggcg tcgaggtctc
      361 gatcgagctg gacgagaacc agttcgtcgg caccgggatc ttcctcttcg cccaggtcct
      421 cgagcgcttc ctcggcctct actcttccgt aaactccttc agccagctcg tcctgtcgag
      481 ccgtcagcgc gagggcgtcg tccgccgctg ggagccgcgc gccggcgagc agatcgtcct
      541 ctgaggccgg atgaaggacc tcctcttcgc cgagccgtac cgcttctcct tcttccaggc
      601 ggtccgcctg ctcgaaagga cgtcgccggg gaaggagggc gtcggccggg ccgtccggcc
      661 cgggaacgag gtcgtccggt tccgctccca cccgtcgctc acgttccccg cgagcgagat
      721 cgccgacctg aaggctccgc ctccgggcgc ggaggaccgg ccgcccgaga tgacggtcgc
      781 gttcatcggc ctcacgggcc cgctcggaat cctcccgcac ccctacacgg agctcgtgcg
      841 ggagcgggcc tcgtacaagg acacggccct ctggtcgttc ctcgacctct tccaccaccg
      901 tttcacctcc ctcttctacc gcgcgtggga gaagtaccgg ttcccgattg ccttcgagag
      961 gtcgggcgag gactccttca ccgagtacgt cttcgacctg atcggcatgg ggacgccggg
     1021 cctgcgcggc cggatgacgg tccgggacca ggcgctcctc ctttacgccg gcctcatcgc
     1081 gcagaagccg cactcgggcg agtcgatcgc ggcggtcctg cgcgactact tcggcgtccc
     1141 ggcggagatc gtccagttcc aggggcagtg gttcccgatg gaggaggaga acgtcacgcg
     1201 gctcggcgcg gcgaactccg agctcgggcg gacggtcgtc gccggctcct cggtcttcgt
     1261 ctcccagtcg aagttccgcg tccgcctcgg gccgctgccg ctttcggcct tcgtctcctt
     1321 cctgccggtc ggcccggcct tccgccccct gacggagctc gtccggttcc tcgccgggct
     1381 cgagttcgac tacgacgtcc agctcgtcct ccggaaggag gaggtccccc cctgctccct
     1441 cgactcgagc gccgcgctcc ccccgatgct cgggtggacg acgtggatca ccacagaaac
     1501 gccccgggag gacgcgggag acgtcgtcct tccggtcgaa aactgaagag acccctctgc
     1561 gggaggacga cgagatgagc gtgaacctga aatccctgat cggccggctg aacgacacct
     1621 gccgccaggc gctcgagggg gcggcgggcc tctgcctctc ccggacgaac tacgacgtgg
     1681 aggtcgagca cctcctcctg aagctcctcg aggcgccgga caccgacctc gcgaagatcc
     1741 tccggcgctt cgagatcgac gcctcgcgct tcgcgaagga cgtggtgcgc gccctcgacc
     1801 ggctcaagac ggggaacgcc cggacgccgg ccctctcgcc gcgcctgccg cgcctgatcg
     1861 agaaggcgtg ggtcgtcgcg tccatcgagt acggcgcctc gcgcgtgagg tcgggccacc
     1921 tcgtccacgt cctcctcacc gacgacgacc tttcgcgcct cgcgcgggag atctcgaagg
     1981 acctcgccgg gatctccgtc gagcagctga cgaagacgtt ccccgacgtc gtcaagggct
     2041 ccgcggagga ccgcgaggcg caggccctct cggaggcgcc cggcgcgggc ggcgggcccg
     2101 acgtcgcgat gggcgtcccc ggcaagacga agtccctcga ccagtacacc gaggacctga
     2161 ccgcgaaggc ccgcgccggg aagatcgacc cgatcctggg ccgcgacttc gagatccgcc
     2221 aggtcatcga catcct
//
LOCUS       DAOTEZ010000586         2230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_41084_length_2230_cov_5.594943, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000586 DAOTEZ010000000
VERSION     DAOTEZ010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41084_length_2230_cov_5.594943"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="P5164_19720"
     CDS             complement(<1..352)
                     /locus_tag="P5164_19720"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRY46169.1"
                     /translation="MKFLRYDDGGLPRWGRLEAGRVTPLDGAPWSGGEAVGPARPYEE
                     LDLLPPVPPGGKVLCIGRNYLAHAKELGNEVPAEPLVFLKPSTSLLPHGGTVVLPPES
                     ERVDFEGELALVIGR"
     gene            complement(349..1215)
                     /gene="dapF"
                     /locus_tag="P5164_19725"
     CDS             complement(349..1215)
                     /gene="dapF"
                     /locus_tag="P5164_19725"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRY46170.1"
                     /translation="MSAETIGGLPRTFWKMAGAGNDFLVFEGAAAIGPREAETIRRLC
                     RRGTGVGADGVLFVSAREASGARREVRADYFNADGGPARFCANGTRCASRFAVLRGLA
                     DAELVTHTGWGAVAATVRADGTVTIRLPEPVALAGEVATFDPEGRVVEPRAAHLTVGV
                     PHLVVFCANGLEVETVDVAALGPPLRRHPDLTDGANVNFVSVRGGSRLSVRSFERGVE
                     AETLSCGSGVVASAVVAGAARGQLAPVSVATRSGSALVVDFRLEGSLAHDVTLTGDAR
                     VVFRGELTEEAV"
     gene            complement(1212..>2230)
                     /locus_tag="P5164_19730"
     CDS             complement(1212..>2230)
                     /locus_tag="P5164_19730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46171.1"
                     /translation="IAPEAPLQVIPARPEPLRVSQEGSDVVLRFPFPSKTIQGSPLTN
                     LTRVTVYREIVPAPPGARAPEPADGAAEREREEKLFRQRAEAIRDLPREGLDEVTVGS
                     ELVVRDPMLPLFVEKRLGRVFLRYGVTATRDRKRVSPLSPLVSIVPKVPPAEPLGLSA
                     TVEEGRVCLEWLPPDGMLDGSTPASVAGYAVYRREEGEESYGEWLGVAGRSPFVDTAV
                     VAGKRYVYTVRAAPTADLPPVLGPAADEVVADTRDVFAPAVPEGLLVLSEEGGNRLVW
                     NPGLARDLAGYSVWVRDGEGTWRRLAERLTDPAFFDRGAPAGRRYAVTAVDAAGNESG
                     RAER"
BASE COUNT          295 a          862 c          814 g          259 t
ORIGIN      
        1 gccgcccgat cacgagggcg agctccccct cgaagtcgac ccgctccgat tccgggggga
       61 ggacgaccgt cccgccgtgg ggcaggagcg acgtcgacgg cttcaggaag acgagcggct
      121 cggccgggac ctcgttcccg agctccttcg cgtgcgcgag gtagttccgg ccgatacaga
      181 ggaccttgcc tcccggcggg accggcggca ggaggtcgag ctcctcgtac ggccgggccg
      241 gaccgacggc ctcgcctccg gaccaggggg cgccgtcgag cggggtgacc cggccggcct
      301 cgagccggcc ccagcggggg agccccccgt cgtcgtatcg caggaacttc atacggcctc
      361 ctccgtcagc tcgccccgga agacgacgcg ggcgtcgccc gtcagcgtca cgtcgtgcgc
      421 gaggctcccc tcgagccgga agtcgacgac gagcgcgctc cccgagcgcg tcgccacgga
      481 gacgggcgcc agctgcccgc gcgcggcccc cgcgacgacc gcggaggcga cgacgcccga
      541 gccgcaggag agcgtctcgg cttcgacgcc ccgctcgaag ctccgcaccg agaggcgcga
      601 gcctcccctc acggagacaa agttcacgtt cgcgccgtcg gtgaggtccg ggtgccgtcg
      661 aagcggcggg ccgagcgcgg ccacgtcgac cgtctcgacc tcgaggccgt tcgcgcagaa
      721 gacgacgagg tgcgggacgc cgaccgtcag gtgggcggcg cgcggctcga cgacgcgccc
      781 ctccgggtcg aacgtcgcga cctcccccgc gagcgcgacc ggctcgggca gccggatcgt
      841 caccgtgccg tcggctcgga cggtcgcggc gaccgccccc cagccggtgt gcgtcacgag
      901 ctccgcgtcg gcgaggccgc ggaggacggc gaaccgcgac gcgcagcggg tcccgttcgc
      961 gcagaatcgc gccggcccgc cgtccgcgtt gaagtagtcg gcccggacct cgcggcgcgc
     1021 gccggaagcc tcgcgcgcgg agacgaagag gacgccgtcg gctccgacgc cggtccccct
     1081 ccggcagagg cgccggatcg tctcggcctc gcgcggcccg atcgcggcgg ccccctcgaa
     1141 gacgaggaag tcgttccccg cgcccgccat cttccagaac gtccgaggca ggccgccgat
     1201 cgtctccgcg ctcatcgctc cgccctcccg ctctcgttcc cggcggcgtc gaccgccgtc
     1261 acggcatagc gcctcccggc gggggcgccc cggtcgaaga aggcggggtc ggtcagtctt
     1321 tcggccagcc gccgccacgt cccctctccg tcccggaccc agacgctgta cccggcgagg
     1381 tcccgcgcga ggccgggatt ccagacgagg cggttccccc cctcttccga aaggacgagc
     1441 agcccctccg ggacggccgg cgcgaagacg tcacgcgtgt cggcgacgac ctcgtcggcc
     1501 gccgggccga ggaccggggg gaggtccgcc gtcggcgcgg cgcggaccgt gtagacgtac
     1561 cgctttcccg cgacgacggc ggtgtcgacg aaaggcgacc ggccggcgac gccgagccac
     1621 tcgccgtagg actcctctcc ctcctcgcga cggtagaccg cgtagccggc gacggaggcc
     1681 ggcgtcgacc cgtcgagcat cccgtcgggc gggagccact cgaggcagac ccgtccctcc
     1741 tcgaccgtcg ccgagaggcc gaggggctcc gccggcggga ccttcgggac gatcgagacg
     1801 aggggcgaga gcggcgagac gcgctttcgg tcccgcgtcg cggtgacccc gtagcggagg
     1861 aagacgcgcc cgagccgctt ctcgacgaag aggggaagca tcgggtctcg cacgacgagc
     1921 tcgctcccga ccgtgacctc gtcgagcccc tcccgcggca ggtcccggat cgcttccgcg
     1981 cgctgccgga agagcttctc ctcccgctcc cgctccgccg ccccgtccgc cggctccggc
     2041 gcccgcgccc ccgggggcgc gggaacgatc tcgcggtaga cggtcacgcg cgtcaggttc
     2101 gtcagcgggg agccctggat cgtcttcgac gggaacggga agcggaggac gacgtcgctc
     2161 ccctcctggg agacccggag cggctcgggg cgcgcgggga tcacctgcag gggcgcttcc
     2221 ggggcgatct
//
LOCUS       DAOTEZ010000587         2221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_41351_length_2221_cov_6.414589, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000587 DAOTEZ010000000
VERSION     DAOTEZ010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41351_length_2221_cov_6.414589"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..>2221)
                     /locus_tag="P5164_19735"
     CDS             complement(15..>2221)
                     /locus_tag="P5164_19735"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HRY46172.1"
                     /translation="GGGAGLSGNPAGPGSVPENVLRLLRGPLRAAFLALPLLLPLPAA
                     AQRPEEPLSALVAEREVRLDGRLGDPLWADAPLLDRFTQREPSEGAPATERTEVRVIV
                     TPRALVVGVVCLDREAATVAARNYERDGDFDADDWVMVVLDGFLDRRNGALFGVNPLG
                     TQWDALVTNEGPEEIVWDAIWESRTSVGPDGWRAEIAIPLSALRYRAGATEIGFNLVR
                     MLRRRQETSSWGAWRREAGPTRISMAGTLTGLRLERRLPLVIKPFLLTGYDRDVRKAG
                     GGFRTGGTFDAGLDAKVGVTPTLVLDATFRTDFAQVEADTQRVNLTRFPIFFPEKRDF
                     FLERIGFTRFGQQEFGEVFYSRRIGLSADGEPVPIVGGARLTGNVGRTEVGVIAIRQD
                     ASAGLPETDFYVARVRHPLGARSSFGAIFTDREGGPPGTEWNRAAGLDLDWKPAETLA
                     FTAFWATTRDPQGKPDTDGWRVSGSYDDGAWNAYSSLKRYDEDFDPGIGFVPRTGITN
                     AFGRAGRRFYPAKGFVREWEVGGELDYFEDTEGDPVGREIRVELRAEGRDASYLEAEP
                     FSSEWDRLDTPFEIRPGIVIPSGGYTNRRRGVEIGTSPAGVLSAAAGVDWGSFYGGQG
                     EKVALEASYRPNPHLRVELTGEYNDVRLPAGSFTASLAGLRTTWNFSRALLLTAYGQV
                     NSDSDVTSLNARLRWMWRPGSDVYVVFNRATGRGLEKRTWQLMLKATWAFLP"
BASE COUNT          309 a          799 c          822 g          291 t
ORIGIN      
        1 ggcctattcc ggcgctacgg caggaacgcc cacgtcgcct tgagcatcag ctgccaggtc
       61 cgcttctcga gccccctgcc ggtggcccgg ttgaagacga cgtagacgtc ggagccgggg
      121 cgccacatcc accggagccg ggcgttgagg ctcgtgacgt cggagtcgga gttgacctgg
      181 ccgtaggcgg tcaggaggag cgcccgggag aagttccagg tcgtccggag gccggccagc
      241 gaggcggtga aggagccggc ggggagccgg acgtcgttgt actcgccggt cagctccacc
      301 cggaggtggg gattcgggcg gtaggaggct tcgagggcga ccttctcccc ctgcccgccg
      361 taaaacgacc cccagtcgac gccggccgcg gcggagagga cgccggccgg gctcgtcccg
      421 atctccacgc cgcgccgccg gttcgtgtag ccgccggacg ggatgacgat cccggggcgg
      481 atctcgaacg gcgtatcgag ccggtcccac tcgctcgaga agggctccgc ctcgaggtag
      541 ctcgcgtcgc gcccttcggc ccggagctcg acgcggatct cccggccgac ggggtcgccc
      601 tccgtgtcct cgaagtagtc gagctccccg cccacctccc actcccgaac gaagcccttc
      661 gccgggtaga agcggcgccc cgcgcggccg aacgcgttcg tgatcccggt ccgcgggacg
      721 aagccgatgc cggggtcgaa gtcctcgtcg tagcgcttca ggctcgagta ggcgttccag
      781 gcgccgtcgt cgtagctccc cgagacgcgc cagccgtcgg tgtcgggctt tccctgcggg
      841 tcgcgcgtcg tggcccagaa cgcggtgaag gcgagcgtct cggccggctt ccagtcgagg
      901 tcgaggcccg cggcgcggtt ccactccgtg ccgggcggcc ccccttcgcg gtccgtgaag
      961 atcgcgccga acgaggagcg cgccccgagc gggtgcctca cgcgggcgac gtagaagtcg
     1021 gtctcgggaa ggcccgcgga ggcgtcctgc cggatggcga tgacgcccac ctcggtccgg
     1081 ccgacgttcc ccgtcaggcg cgccccgccg acgatcggca ccggctcgcc gtcggccgag
     1141 aggccgatcc gccgggagta gaagacctcg ccgaactcct gctggccgaa gcgggtgaag
     1201 ccgatccgct cgaggaagaa gtcgcgcttc tcggggaaga agatcgggaa gcgcgtcagg
     1261 ttcacgcgct gcgtgtcggc ctccacctgg gcgaagtcgg tccggaacgt cgcgtcgagg
     1321 acgagcgtcg gcgtgacgcc gaccttcgcg tcgagccccg cgtcgaacgt cccgccggtc
     1381 cggaagccgc ctccggcctt ccgcacgtcg cggtcgtagc cggtcaggag gaacggcttg
     1441 atcacgagcg gaaggcgccg ctccagccgg aggccggtga gcgtgccggc catcgagatg
     1501 cgcgtcgggc ccgcctccct ccgccaggcg ccccaggagg aggtctcctg acggcgcctc
     1561 agcatgcgga cgaggttgaa gccgatctcc gtcgcccccg cccggtagcg gagcgccgag
     1621 agcggaatcg cgatctcggc gcgccacccg tcggggccga ccgacgtgcg cgactcccag
     1681 atcgcgtccc acacgatctc ctccggcccc tcgttcgtca cgagcgcgtc ccactgcgtg
     1741 ccgagcgggt tgacgccgaa gagcgcgccg ttccgccggt cgaggaaccc gtcgaggacg
     1801 accatcaccc agtcgtcggc gtcgaagtcc ccgtcccgct cgtagttccg ggcggcgacg
     1861 gtcgccgcct cgcggtcgag gcagacgacg ccgacgacga gggcgcgcgg cgtgacgatc
     1921 acgcggacct cggtccgctc cgtcgcgggc gccccctcgg acggctcgcg ctgcgtgaag
     1981 cggtcgagga gaggggcgtc ggcccagagc gggtcgccga ggcggccgtc caggcggacc
     2041 tcgcgctcgg cgacgagcgc ggagagcggc tcctccggcc gctgggccgc ggccggaaga
     2101 gggaggagaa gcgggagggc gaggaacgcg gcccggagcg ggcctcggag gagccgcaag
     2161 acgttctctg gtacggagcc cggtccggcc gggtttccgc tcagcccagc accgcctcca
     2221 g
//
LOCUS       DAOTEZ010000588         2187 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_42436_length_2187_cov_6.189493, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000588 DAOTEZ010000000
VERSION     DAOTEZ010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42436_length_2187_cov_6.189493"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..144
                     /gene="rpsU"
                     /locus_tag="P5164_19740"
     CDS             <1..144
                     /gene="rpsU"
                     /locus_tag="P5164_19740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRY46173.1"
                     /translation="KCEKSGILSELKKRQHYEKPSTKRKRKAIAARKKMLRKLAEERR
                     AGV"
     gene            161..>2187
                     /locus_tag="P5164_19745"
     CDS             161..>2187
                     /locus_tag="P5164_19745"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46174.1"
                     /translation="MIHTEVDWPGVLRLAGRFCSSERGRERLLAATPSGDPAEVRRRA
                     GVTRDLVARTGLRPPVRIFGLDEGVPLVSRLGAAGQALAPEELLDLFALVERSEEARV
                     AVPLEGLELPDLTALLAPLGDLEDLLGERDYTFEPDGRVRDTASARLASIRAAIVRLR
                     RDVVRRLEELVRARPDALADGYVTEKGGRYCLPVRSDRRESVAGIVHEKSGSGQTLFV
                     EPLAVVEANNALSEAFEEEREEIHRILVALTARFAARKRELSAAVEVLTELDGYQARA
                     ELARRSQGVFPETDGPLVLKKARHPLLDRRLAPLREEVFGERAEERGSDAVPLDLVLD
                     AGQRLVLLSGPNAGGKTVAMKTVGLFALLAQSGFAVPADPGTSLPVFDRLLVVAGDAQ
                     DLLGDLSSFAGAMTRTAAALAAATPRSLVLLDELGSGTDPDEGGAIAMSVLEEDLRRG
                     GLTVATTHLAAVKEWAHDRPDVLSAAMEFDESLGRPTFRVRPGATGRSRALAVAERSG
                     IPRRVLEAARERLGSGWAAADAALERLESETRAARAEAERLRAEAAALASRRDALERE
                     AAALATEKQRLRERAKTEIDRAIEALRERTRRELDRIREEARAGRAVSRGALMTVVGE
                     ARKDAEALFEMAAPPEPSGPVVVGERVKVVPFGTTGKLLALDEAKGQAEVEV"
BASE COUNT          277 a          827 c          815 g          268 t
ORIGIN      
        1 aagtgcgaga agtcggggat cctgtcggag ctcaagaagc gccagcacta cgagaagccg
       61 tcgaccaagc ggaagcgcaa agcgatcgcc gcgaggaaga agatgctgcg gaagctggcc
      121 gaggagcgcc gcgcgggagt ctgatcccga gacccctttc atgattcaca cggaagtcga
      181 ctggcccggc gtgcttcggc tcgccgggcg tttctgctct tcggagcggg gacgcgagcg
      241 gctcctcgct gcgaccccct cgggcgaccc cgccgaggtg cggcgccgtg cgggagtcac
      301 ccgggatctc gtcgcccgca ccggccttcg cccgcccgtc cggatcttcg gcctcgacga
      361 gggggtcccg ctcgtctcgc gcctcggcgc cgccgggcag gcgctcgcgc cggaggagct
      421 cctcgacctc ttcgcgctcg tcgagcgttc cgaggaggcc cgggtcgccg tccctctcga
      481 aggcctcgag ctccccgacc tgaccgccct cctcgccccc ctcggcgacc tcgaggacct
      541 cctcggcgag cgcgactaca ccttcgagcc cgacggcagg gtccgcgaca ccgcttcggc
      601 gcgcctcgcc tcgatccgcg ccgcgatcgt ccgcctccgg cgcgacgtcg tgaggcgcct
      661 cgaggagctc gtccgcgcgc ggcccgacgc gctcgccgac ggctacgtca cggagaaggg
      721 ggggcgctac tgcctcccgg tccgctccga ccggcgcgag agcgtcgccg ggatcgtcca
      781 cgagaagtcg ggctcggggc agacgctctt cgtcgagcct ctcgcggtcg tcgaggcgaa
      841 caacgcgctc tcggaggcct tcgaggagga gcgcgaggag atccaccgga tcctcgtcgc
      901 gctcacggcg cggttcgccg cgcgaaagag ggagctctcc gcagccgtcg aggtcctcac
      961 ggagctggac ggctaccagg cgcgtgccga gctcgcccgc cggtcgcagg gagtcttccc
     1021 cgagacggac ggcccgctcg tcctgaagaa ggcccggcac ccgctcctcg accggcgcct
     1081 cgcgccgctc cgcgaggagg tcttcggcga gagggccgag gagcgcggct ccgacgccgt
     1141 cccgctcgac ctcgtcctcg acgcggggca gcgcctcgtc ctcctctcgg gcccgaacgc
     1201 gggcggcaag acggtggcga tgaagaccgt cggcctcttc gcgctcctcg cgcagtcggg
     1261 cttcgccgtc ccggccgacc ccggcacctc cctccccgtc ttcgacaggc tcctcgtcgt
     1321 cgcgggcgac gcccaggacc tcctcggaga cctctcgtcc ttcgccggcg cgatgacgcg
     1381 caccgcggcg gcgctcgccg cggcgacgcc ccggagcctc gtcctcctcg acgagctcgg
     1441 gagcggcacc gaccccgacg aggggggcgc catcgcgatg tcggtcctcg aggaggacct
     1501 gcgtcggggc gggctgacgg tcgccacgac gcacctcgcc gcggtgaagg agtgggcgca
     1561 cgaccgcccc gacgtcctct cggccgcgat ggagttcgac gagagcctcg gccgcccgac
     1621 gttccgcgtg cggcccggag ccaccgggcg ctcacgcgcc ctcgccgtcg cggagcgctc
     1681 cggcatcccg cggcgcgtcc tcgaggcggc gcgcgagcgg ctcggctcgg ggtgggccgc
     1741 ggccgacgcg gcgctcgagc gcctcgagtc ggagacgcgg gcggcgcggg cggaggcgga
     1801 gaggctccgc gccgaggctg cggccctcgc ctcgcggcgc gacgccctcg agcgggaggc
     1861 ggccgcgctc gcgaccgaga agcagcgcct gcgcgagcgg gcgaaaaccg agatcgaccg
     1921 ggcgatcgag gcgctccgcg agcggacgcg ccgcgagctg gaccggatcc gcgaggaggc
     1981 gcgcgcgggg cgcgccgtct cccgcggcgc gctgatgacc gtcgtcggcg aggcgcggaa
     2041 ggacgccgaa gcgctcttcg agatggcggc gccgccggag ccgtcgggcc ccgtcgtcgt
     2101 cggggagagg gtgaaggtcg tcccgttcgg gacgacgggg aagctcctcg cgctcgacga
     2161 ggcgaagggg caggccgagg tcgaggt
//
LOCUS       DAOTEZ010000589         2179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_42711_length_2179_cov_3.759416, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000589 DAOTEZ010000000
VERSION     DAOTEZ010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42711_length_2179_cov_3.759416"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="P5164_19750"
     CDS             complement(<1..505)
                     /locus_tag="P5164_19750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRY46175.1"
                     /translation="MDFRALAATRYSVRAYRPDPVPDALLEELLETVRLAPSACNRQP
                     FRVVVLPTRGRDGDLLRIYPKPWFVEAPLVLAVCAVPAEAWTRRRYDGWGAAETDATI
                     AMDHLLLAAADRGLGTCWVAAFDPAAAREVLALPDGIVPVAFTPLGWPADAPGPKERR
                     PLTELVIR"
     gene            complement(536..1177)
                     /locus_tag="P5164_19755"
     CDS             complement(536..1177)
                     /locus_tag="P5164_19755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46176.1"
                     /translation="MSPPLVAALVLLALAATLGYFRGVRKNRAIAASIAGGLEEALRP
                     ASTEYVNVGGAIGHNFTFRLAGEWTEARGVFTLSPRHSLLYLPLSRLLGVRDRLFLNV
                     FTARPFRGEGHVVRDSDERRAGIEGRSEMERREVEIAGLRFVLLWRGADLSGALEELL
                     RGMPDPARLRHFCAYPGTGTFFLRTEPAPGSVAGDVSSVLLRTGPFLAASPES"
     gene            complement(1174..2103)
                     /locus_tag="P5164_19760"
     CDS             complement(1174..2103)
                     /locus_tag="P5164_19760"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HRY46177.1"
                     /translation="MARNAFFLGKGGTGKTTLSAALAVSLARAGRRVLAVSLDPAHNL
                     GDLLGVLLGEVPVRVEERLEALEVDLGAWVERHLAASREELKATYAYTSALNVDSLFD
                     LLRLSPGTEEHAVLLAIDHVDRVLAPSYDVVVWDTPPTGLTLRFLAMPSVSARWVAEL
                     RGLRERILGRRQAIARVNTESAAAAGAVTPADDPVHARLGAMRERLGRLQDLFARRSL
                     LAVVVNPDLLSVAEASRISEALARLDIPLSAVCLNSRDGAGSPTSLAPPLAHLPRVET
                     GYLAGPSRSRADLLLLDLAPLAARLFARPEAAP"
BASE COUNT          318 a          716 c          909 g          236 t
ORIGIN      
        1 tccgaatcac gagctctgtc agcgggcgcc tctccttcgg acccggggca tcggccggcc
       61 agccgagggg tgtgaaggcg acggggacga tcccgtccgg gagcgcgagg acctcgcgcg
      121 ccgcggccgg gtcgaatgcg gcgacccagc aggtcccgag gccgcggtcg gccgccgcga
      181 ggaggaggtg gtccatcgcg atcgtcgcgt cggtctcggc ggcgccccag ccgtcgtacc
      241 ggcgccgggt ccacgcctcc gccggcacgg cgcagaccgc gaggacgagc ggcgcctcga
      301 cgaaccaggg tttggggtag atccggagga gatcgccgtc gcggccgcgc gtcggaagga
      361 cgacgacccg gaacggctgc cggttgcagg ccgaaggagc gagccggacc gtctcgagga
      421 gctcctcgag gagggcgtcc gggacggggt cgggacggta ggcgcggacg ctgtagcggg
      481 ttgcggcgag ggcgcggaag tccatcggcc gatcgtagcg ccggacgcgc gaacgctacg
      541 actcgggcga cgccgcgagg aaggggccgg tccgcaggag cacggaggag acgtccccgg
      601 cgacggagcc cggtgccggc tccgtccgga ggaagaaggt gcccgtcccg gggtaggcgc
      661 agaagtgccg gaggcgcgcc gggtcgggca tcccgcggag gagctcctcg agggcgccgg
      721 agaggtcggc gccgcgccag aggaggacga accgcaggcc cgcgatctcg acctcacgcc
      781 gctccatctc ggaacgtcct tcgattccgg cgcgacgctc gtcggagtcg cggacgacgt
      841 gcccctcgcc ccggaacggg cgcgcggtga agacgttcag gaagagccgg tcgcggacgc
      901 cgaggaggcg ggagaggggg aggtagagga gcgagtggcg cgggctcagg gtgaagacgc
      961 cgcgcgcctc cgtccactcg cccgcgaggc ggaacgtgaa gttgtggccg atggcccctc
     1021 cgacgttcac gtactcggtc gaagccggcc ggagcgcctc ctcgaggccg cccgcgatcg
     1081 aggcggcgat cgcgcggttc ttccgcacgc ctcggaagta gccgagcgtg gcggcgaggg
     1141 cgaggaggac gagggccgcg acgagcgggg ggctcacggg gcggcctcgg ggcgtgcgaa
     1201 cagccgtgcg gcgagcggcg cgaggtcgag gagaaggagg tcagcccggg agcgggacgg
     1261 cccggcgagg tatccggtct cgacgcgggg caggtgcgcg agcggcggag cgaggctcgt
     1321 cggagagcct gccccgtccc ggctgttgag gcagacggcg gagagcggaa tgtcgaggcg
     1381 ggccagcgcc tccgagatcc gcgacgcttc ggcgacggag aggaggtcgg ggttgacgac
     1441 gacggcgagg aggctccgcc gcgcgaagag gtcctggagc cgcccgagcc gttcccgcat
     1501 cgctccgagc ctcgcgtgga cggggtcgtc cgccggcgtc acggcccctg ccgcggcagc
     1561 cgattccgtg ttcacgcggg cgatcgcctg ccgacggccg aggatcctct cgcgcaggcc
     1621 gcggagctcg gcgacccagc gggccgagac gctcggcatg gcgaggaatc ggagcgtgag
     1681 gccggtcggc ggcgtgtccc agacgacgac gtcgtaggac ggggcgagga cgcggtcgac
     1741 gtgatcgatc gcgaggagga cggcgtgctc ctccgttccg ggcgagaggc ggaggaggtc
     1801 gaagagagag tcgacgttca gcgcggaggt gtaggcgtag gtcgccttca gctcctcgcg
     1861 gctcgcggcg aggtggcgct cgacccaggc ccccaggtcg acctcgagag cctccaggcg
     1921 ctcctcgacc cgaaccggca cctcgccgag caggacgccg aggaggtcgc cgaggttgtg
     1981 ggccgggtcg agcgagacgg cgaggacgcg ccgtcccgcg cgggcgaggg agaccgcgag
     2041 cgccgcggag agcgtcgtct tgccggtgcc ccccttcccg aggaagaacg cgtttcgcgc
     2101 cacgcgccgc tccggcctca gcccgcgtcg agcgtgccgg cgagcgcggc gagcgggtcg
     2161 tgcgcgaggt cggcgcgcg
//
LOCUS       DAOTEZ010000590         2178 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_42744_length_2178_cov_7.110692, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000590 DAOTEZ010000000
VERSION     DAOTEZ010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42744_length_2178_cov_7.110692"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..>2178
                     /locus_tag="P5164_19765"
     CDS             185..>2178
                     /locus_tag="P5164_19765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HRY46178.1"
                     /translation="MRAHATAHAHAHQDHSDTPAPAAPAAGGAVRWICPMDPEVVSDG
                     PGDCPVCGMALEPEMPTAEVESPELADMTRRLKVATALGLPVLVLAMSDMVPGQPLQH
                     AVSPWLLTLAQALLATPVVAWAGAPFFRRAWASVVNRSPNMFTLIGLGVAAAWLYSAV
                     ALAVAALRPDLVPEVYRGHGGAPLVYFEAAAVITALALLGQVLELKARSRTSSAIRAL
                     LDLSPKTARRIEAEGREVDVPVEHVAAGDRLRVRPGERVPVDGTIEDGTSWVDESMIT
                     GEPIPVEKGAGSAVTGGTVNGAGGFVMVAERVGEGTTLARIVKLVAEAQRSRAAIQQL
                     ADRVSAWFVPAVVVASFVTFALWMALGPEPRFVHALASAIAVVIIACPCALGLATPLS
                     IMVATGRGALSGVLVKNADALERLAAVDVLVVDKTGTLTEGRPAVAALEAVAPFTKDE
                     VVRLAAALERASEHPLGAAIVAAAGAGPLPLVSEFRSVTGRGVAGLVSGHDVLVGSLR
                     FLEEAGVETSALAALATAHQEEGRTAVTVAVDGRPAGLVAVADPVKASAPAALAALRA
                     DGIEIVLLTGDARRSAAAVAKSLGIARFEAEVLPEEKGAVVERLRAEGRVVAMAGDGV
                     NDAPALAAADVGIAMGTGTDVAIESASVTLLHGDLAGLVRA"
BASE COUNT          249 a          867 c          781 g          281 t
ORIGIN      
        1 cgccgacacc gacgggtggt tccgccactc cgaggggacc gtggccgggc ccgcggacgc
       61 cgcccgcctc gcggcggacg ggatcgagcc gtaaggcgtg aactgacggc ggagcggggc
      121 gggccggact gtaaggtccc ggcccgcccc gcgtctcctc ctgtgaaacc gacaggaggc
      181 gagcatgaga gcccacgcaa ccgcccacgc ccacgcccac caggaccatt ccgacacgcc
      241 cgccccggcc gcccccgcgg ccggcggcgc ggtccggtgg atctgcccga tggaccccga
      301 ggtcgtctcc gacggcccgg gcgactgccc ggtctgcggc atggcgctcg agcccgagat
      361 gccgacggcg gaggtcgagt ctcccgagct cgccgacatg acgcggaggc tgaaggtcgc
      421 gaccgcgctc ggcctgccgg tcctcgtcct cgcgatgtcg gacatggtcc cgggccagcc
      481 gctccagcac gccgtctcgc cctggctcct cacgctcgcc caggccctcc tcgcgacgcc
      541 ggtcgtcgcc tgggccgggg cgccgttctt ccggcgcgcg tgggcctccg tcgtgaaccg
      601 cagcccgaac atgttcacgc tcatcggcct cggcgtcgcg gccgcgtggc tctacagcgc
      661 cgtcgccctc gccgtcgccg ctctccgtcc cgacctcgtc cccgaggtct accgcgggca
      721 cggcggcgcg ccgctcgtct acttcgaggc ggcggccgtc atcacggcgc tcgccctcct
      781 cggccaggtc ctcgagctga aggcccggag ccggacgagc tcggccatcc gcgccctcct
      841 cgacctctcg ccgaagacgg cgcggaggat cgaggccgag ggccgcgagg tggacgtccc
      901 cgtcgagcac gtcgcggcgg gcgaccgcct ccgcgtgcga cccggcgagc gcgtcccggt
      961 ggacgggacg atcgaggacg ggacctcctg ggtcgacgag tcgatgatca ccggcgagcc
     1021 gatcccggtc gagaagggcg cggggagcgc cgtcacgggc gggaccgtaa acggcgccgg
     1081 cggcttcgtc atggtcgccg agcgggtcgg cgaggggacg acgctcgcgc ggatcgtgaa
     1141 gctcgtcgcc gaagcccaga ggagccgcgc cgcgatccag cagctcgcgg accgtgtctc
     1201 ggcctggttc gtcccggcgg tcgtggttgc gtcgttcgtg accttcgcgc tctggatggc
     1261 cctcgggccc gagccccggt tcgtccacgc cctcgcctcg gcgatcgcgg tcgtcatcat
     1321 cgcctgcccg tgcgccctcg gcctcgcgac gcccctctcg atcatggtcg ccacgggccg
     1381 cggcgctctc tccggcgtcc tcgtgaagaa cgccgacgcc ctcgagcggc tcgccgccgt
     1441 ggacgtcctc gtcgtcgaca agaccggcac gctgaccgaa gggcggcccg ccgtcgcggc
     1501 cctcgaggcc gtcgcgccgt tcacgaagga cgaggtcgtc cgtctcgccg ccgccctcga
     1561 gcgcgcgagc gagcacccgc tcggcgcggc catcgtggcc gccgcgggcg ccggtccgct
     1621 tcccctggtc tcggagttcc gatccgtcac gggccgcggc gtcgcgggcc tcgtctcggg
     1681 ccacgacgtc ctcgtcggga gcctgcgctt cctcgaggaa gccggggtcg agacgtcggc
     1741 gctcgccgcc ctcgcgacgg cccaccagga agaaggccgg accgccgtca ccgtcgccgt
     1801 cgacggccgc cccgcgggcc tcgtcgccgt cgccgacccg gtgaaggcgt cggccccggc
     1861 cgccctcgcg gccctccgcg ccgacggcat cgagatcgtc ctcctgacgg gagacgcgcg
     1921 ccgctcggcc gccgccgtcg cgaagtcgct cgggatcgca cggttcgagg ccgaggtcct
     1981 tcccgaggag aagggggccg tcgtcgagag gctccgcgcc gaaggccggg tcgtcgcgat
     2041 ggcgggcgac ggcgtgaacg acgccccggc cctcgccgcg gccgacgtcg gcatcgcgat
     2101 ggggaccggg accgacgtcg ccatcgagag cgcctccgtg acgctcctcc acggcgacct
     2161 cgccggcctc gtccgcgc
//
LOCUS       DAOTEZ010000591         2132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_44262_length_2132_cov_6.131440, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000591 DAOTEZ010000000
VERSION     DAOTEZ010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44262_length_2132_cov_6.131440"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="P5164_19770"
     CDS             <1..926
                     /locus_tag="P5164_19770"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY46179.1"
                     /translation="RHDRPSRPAPWLDEVVFRRFGSTAEGLRLMRRGVPVAVADPSAA
                     MVAQARSDPRLRVVTTDAGSLTYLAVGFSAGAGPLGDPRVRRALRLSLDLPALVAEGT
                     IAGGTPAGRVVPPGSFGFDPSRRPPRRDLAAARRLLEEAGYPNGFELDLDVGPNSRRA
                     GRALADQVAEAGIRLRVVVRSPDDFVSRIDGRSPLYLYSWFVGRDAGQALRNAFHTRD
                     AARGLGSMNRTGYSNAAVDAALVGLAAAAQAEERLARLREVSDLLDGDLPWIPLYSAR
                     QVRLLPAWLDLGWRPDGLLVIADARPAGGGR"
     gene            complement(952..>2132)
                     /locus_tag="P5164_19775"
     CDS             complement(952..>2132)
                     /locus_tag="P5164_19775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRY46180.1"
                     /translation="DEVWRRYRDFFYVRNMHGYDWKAIGERYRALLPHVSHRSDLTYL
                     LGEMVAELNVGHAYVEEGDVERPARAKVGLPGARFELDEKAGLYRIAKVFRGHNEEPK
                     YRAPLTEVGVDARVGDYVLAVDGEPLAASDNPYRLLQHKTHPVTLTLNAKPTAGGARK
                     VTYRPVESEASLLYLDWVLGNVEKVSKLSSGRVGYLHVPDMGAPGIYEFLKWYYPQIR
                     KEGLVVDVRSNGGGNVSQWILERLDSKLLGTRFGFSSDLPMTYPYAVFHGHLAALINE
                     TSASDGDIFPARFRKAGLGPLIGKRTWGGVVGISGRGPLIDGGQVFVPQQATNDVDGR
                     YIIEGEGVSPDIEVENDPASVAAGRDPQLERAVAEVLKAIEKEPKTLPKRPADPVKTK
                     "
BASE COUNT          258 a          798 c          760 g          316 t
ORIGIN      
        1 cgcgccacga tcggccctcc cgccccgcgc cctggctcga cgaggtcgtc ttccggcggt
       61 tcgggagcac cgccgagggc cttcgcctca tgcgacgcgg cgtcccggtc gcggtcgccg
      121 acccgtcggc cgcgatggtc gcccaggcgc ggagcgaccc gcgactccgc gtcgtcacga
      181 cggacgccgg gagcctcacc tacctcgcgg tcggcttctc ggccggggcc ggcccgctcg
      241 gagacccgcg cgtccgtcgg gcgctccgcc tctccctcga cctccccgcc ctcgtggcgg
      301 aggggacgat cgcgggaggg accccggccg gacgcgtcgt cccgccgggc agcttcggct
      361 tcgacccatc gcgtcgaccg ccccggcggg acctggcggc ggcgcgccgg ctgctcgagg
      421 aggccggcta cccgaacggc ttcgagctcg acctcgacgt cggcccgaac tcgcggcggg
      481 ccggccgggc tctcgcggac caggtggccg aggccggaat ccggctccga gtcgtggtcc
      541 gaagccccga cgacttcgta tcgcggatcg acggccggag cccgctctac ctctacagct
      601 ggttcgtcgg ccgcgacgcc gggcaggcgc tccggaacgc cttccacacg agggacgccg
      661 cgcgcggcct cggctcgatg aacaggaccg gatactcgaa cgccgccgtc gacgcggccc
      721 tcgtcggcct cgccgccgcg gcgcaggcgg aggaacgcct cgctcgcctc cgggaggtct
      781 cggacctcct cgacggggac ctcccctgga ttccgctcta ctccgcccgc caggtccggc
      841 tcctcccggc ctggctcgat ctcggatgga ggcccgacgg cctcctcgtg atcgccgacg
      901 cgcggccggc cggcggcggg cgctgacccg ccgccggccc ccggcggaga cctacttcgt
      961 cttgaccggg tccgcgggcc gcttcgggag cgtcttcggc tccttctcga tcgccttcag
     1021 gacctccgcg accgctcgct cgagctgcgg gtcgcggccg gccgcgacgg aggccgggtc
     1081 gttctcgacc tcgatgtcgg gcgagacgcc ttcgccttcg atgatgtagc ggccgtcgac
     1141 gtcgttcgtc gcctgctgcg ggacgaagac ctggccgccg tcgatcagcg gcccgcgccc
     1201 cgagatgccg acgacgccgc cccacgtcct cttcccgatc agggggccga ggcccgcctt
     1261 ccggaagcgc gcggggaaga tgtcgccgtc cgacgccgac gtctcgttga tgagcgcggc
     1321 caggtggccg tggaagaccg cgtacgggta ggtcatcggg aggtcgctcg agaagccgaa
     1381 gcgggtcccg aggagcttcg agtcgagcct ctcgaggatc cactgcgaga cgttcccgcc
     1441 gccgttcgag cggacgtcga cgacgagccc ctccttccgg atctgcgggt agtaccactt
     1501 gaggaactcg tagatccccg gcgcccccat gtcggggacg tgcaggtatc cgacgcgccc
     1561 gctcgagagc ttcgagacct tctccacgtt gccgagcacc cagtcgaggt agaggaggct
     1621 cgcctcgctc tccaccggcc ggtaggtgac cttcctggcc ccgccggcgg tcggcttcgc
     1681 gttgagcgtg agggtgacgg ggtgggtctt gtgctgcagg agacgatagg ggttgtcgct
     1741 cgcggcgagc ggctcgccgt cgacggcgag gacgtagtcg ccgacgcgcg cgtcgacccc
     1801 gacctcggtc aggggcgcgc ggtacttcgg ctcctcgttg tggccgcgga agaccttcgc
     1861 gatccggtag aggcccgcct tctcgtcgag ctcgaagcgg gcgcccggga ggccgacctt
     1921 ggcgcgcgcc gggcgctcga cgtccccctc ctcgacgtac gcatggccga cgttcagctc
     1981 cgcgaccatc tcgccgagga ggtacgtcag gtcggagcgg tgggagacgt gcggcaggag
     2041 cgcccggtag cgctcgccga tcgccttcca gtcgtagccg tgcatgttgc ggacgtagaa
     2101 gaagtcgcgg tagcggcgcc agacctcgtc ga
//
LOCUS       DAOTEZ010000592         2129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_44375_length_2129_cov_5.500482, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000592 DAOTEZ010000000
VERSION     DAOTEZ010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44375_length_2129_cov_5.500482"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="P5164_19780"
     CDS             <1..897
                     /locus_tag="P5164_19780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HRY46181.1"
                     /translation="DNVTYFGDGTDEAQMVYNFALPPLVLHAFVTGSAEALSSWAAEL
                     APPSETTAFFNFLDSHDGIGLMGARGILPEEEIGKLCARVKAVGGLVSMRTGEGGAPS
                     PYELNVTWWSALNDAEADGPLDRKVDRFVASRAIALSLRGVPGIYLPSFFGARNDHEA
                     VKRDGQARSINRSAMREEWLFDQFSDPSSIPSRTASRFIDLLGRRAAEPAFHPAAGQR
                     VLRLDPRLFALVRTPRAGGAPIVVVVDVSGTEVEASLPLGDAGLAATELVDLVSGRTF
                     ASDGGVVPLRLRPYQLLWLRPR"
     gene            complement(986..1732)
                     /locus_tag="P5164_19785"
     CDS             complement(986..1732)
                     /locus_tag="P5164_19785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008821421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HRY46182.1"
                     /translation="MTTPVRPYGDTLDDGAVQLSFTLPVPFGPRGREAARLLVEKLGF
                     RHAEVVHSAPLAEGFSFYVAYGRTDVSVDFEAVRVEEATGEQVYTMDEACAVIREKLG
                     RKLVVVGACTGFDAHTVGIDAIMNMKGYNHHYGLERYSEVEAHNLGAQVPNERLIDYA
                     VSVNADAILVSQIVTQKDVHIRNLTELIELLEAKGLRERFVVCAGGTRIGNKLAVELG
                     YDAGFGPGTYADHVATFVIERLVERAATRA"
     gene            complement(1729..>2129)
                     /locus_tag="P5164_19790"
     CDS             complement(1729..>2129)
                     /locus_tag="P5164_19790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HRY46183.1"
                     /translation="FNFASKLTGQGIHLLGMLTEAIHTPFMQDRYLALENAKYVMGSM
                     ASLGDEITFRPDGVVVRRANQVLEEAVAFLEKVEGTGLMDAIATGLFADVKRPRDGGK
                     GLDGLRKKGEGYWNPFETHLTRELGLGGGR"
BASE COUNT          277 a          773 c          743 g          336 t
ORIGIN      
        1 gacaacgtca cctacttcgg cgacgggacg gacgaggcgc agatggtcta caacttcgcg
       61 ctgccgcccc tcgtcctcca cgccttcgtg accggctcgg ccgaggccct ctcgagctgg
      121 gccgcggagc tcgccccgcc gtcggagacg acggcgttct tcaacttcct cgactcgcac
      181 gacggcatcg gcctcatggg cgcgcgcggg atcctcccgg aagaggagat cgggaagctc
      241 tgcgcccgcg tgaaggccgt cggcggcctc gtctcgatgc ggacgggcga aggcggggcg
      301 ccgagcccct acgagctgaa cgtcacctgg tggagcgctc tgaacgacgc ggaggcggac
      361 gggccgctcg accgaaaggt cgaccggttc gtcgcctcgc gcgcgatcgc cctctcgctg
      421 cggggcgtcc ccggtatcta cctcccgagc ttcttcggcg cgcggaacga ccacgaggcg
      481 gtgaagcggg acgggcaggc gcgctcgatc aatcgctccg cgatgaggga ggagtggctc
      541 ttcgaccagt tctccgaccc ctcctcgatc ccctcgcgca ccgccagccg gttcatcgac
      601 ctcctcggcc gccgggcggc cgagccggcc tttcacccgg cagcggggca gcgcgtgctc
      661 cgcctcgacc cgcggctctt cgccctcgtg cggacgccgc gcgccggcgg ggccccgatc
      721 gtcgtggtcg tcgacgtctc cgggacggag gtcgaggcct ccctcccgct cggcgacgcc
      781 ggcctcgccg cgacggagct cgtcgacctc gtctcgggcc ggaccttcgc gtcggacgga
      841 ggcgtcgtcc ccctccgcct ccgcccctac cagctcctct ggctccgccc tcgatagagc
      901 ggggaggggc tctgaagggg gtctggtcta cgttctacgc tcggcgcgag cggccggggc
      961 aggcccggcc gctcgcgggg ccacgtcagg cgcgggtggc ggcccgctcg acgagccgct
     1021 cgatcacgaa cgtcgcgacg tggtcggcgt aggtcccggg gccgaagccg gcgtcgtagc
     1081 cgagctcgac cgcgagcttg ttcccgatcc gggtgccgcc cgcgcagacg acgaaccgct
     1141 cccggagccc cttcgcctcg aggagctcga tcagctcggt caggttccgg atgtggacgt
     1201 ccttctgcgt gacgatctgg ctgacgagga tcgcgtcggc gttcaccgag acggcgtagt
     1261 cgatcagccg ctcgttcggc acctgggcgc cgaggttgtg cgcctcgacc tccgagtagc
     1321 gctcgaggcc gtagtggtgg ttgtacccct tcatgttcat gatggcgtcg atcccgacgg
     1381 tgtgggcgtc gaagccggtg caggcgccga cgacgacgag cttgcgcccg agcttctccc
     1441 ggatgacggc gcaggcctcg tccatcgtgt agacctgctc gcccgtcgcc tcctcgacgc
     1501 ggacggcctc gaagtcgacg gagacgtcgg tccgcccgta ggcgacgtag aagctgaagc
     1561 cctccgcgag cggggcggag tggacgacct cggcgtgccg gaagccgagc ttctcgacga
     1621 ggagccgggc cgcctcgcgc ccgcgcgggc cgaacgggac cgggagcgtg aaggagagct
     1681 ggacggcgcc gtcgtccagg gtgtcgccgt acggtctcac gggtgtcgtc atcgcccgcc
     1741 tccgagcccc agctcgcgcg tcaggtgcgt ctcgaacggg ttccagtacc cctcgccctt
     1801 cttccggagg ccgtcgagcc ccttgcctcc gtcgcggggg cgcttcacgt cggcgaagag
     1861 ccccgtggcg atggcgtcca tcagcccggt cccttcgacc ttctcgagga acgccaccgc
     1921 ctcctcgagg acctggttcg cccgcctcac gacgacgccg tccggccgga acgtgatctc
     1981 gtcgccgagg ctcgccatgc tccccatgac gtacttcgcg ttctcgaggg cgaggtaacg
     2041 gtcctgcatg aacggcgtgt ggatcgcctc ggtgagcatc ccgaggaggt ggatcccctg
     2101 gccggtcagc ttcgaggcga agttgaaga
//
LOCUS       DAOTEZ010000593         2114 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_44880_length_2114_cov_7.140845, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000593 DAOTEZ010000000
VERSION     DAOTEZ010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44880_length_2114_cov_7.140845"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="P5164_19795"
     CDS             complement(<1..448)
                     /locus_tag="P5164_19795"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HRY46184.1"
                     /translation="MSGAPALSAARRENRALTHEAGANFSVGFRFLPKERRHAVYAAY
                     AWCRVADDAVDEGDPAGAPARLDAWEEELDAVYGGSPASAAGVALAGSLPRFPIPKAA
                     FQGLLAGCRQDLVKTRYATFHELLGYCDLVASTISTISLAIFGGLGN"
     gene            complement(445..1581)
                     /locus_tag="P5164_19800"
     CDS             complement(445..1581)
                     /locus_tag="P5164_19800"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HRY46185.1"
                     /translation="MSAPPPYSIAGAGPAGLTAATLLARAGRKVVVHERNATVGARFV
                     GDLQVIEDASDPRETVPAMFARLGLAAPAWVPATTAEFFDAKLRRSVVSSKEPYAWFV
                     RRGPDAGTLDTTLLGGALEAGAEVRFRSRLEPKDADLVATGPQTPDGLARETAFESDG
                     ATRVRVLFDPAVAPGGYAYLFTVGGRGTFGCAIVRDLGGIDRYFERAREKLLSVEKVP
                     MEGARDAYSFMNFALAPEPLREGRPAAGEAGAFQDYLFGLGMRYAISSGALAAEALLA
                     GGDFAALARASFARRQRVSVANRFLYEAGGAFGLSLFARLAAHADFRALLAGFYRPGA
                     LRLALAPIVAALWRNRGRCAHRLPEHWCRRREREIREVALGRIG"
     gene            1732..>2114
                     /locus_tag="P5164_19805"
     CDS             1732..>2114
                     /locus_tag="P5164_19805"
                     /inference="COORDINATES: protein motif:HMM:NF040068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HRY46186.1"
                     /translation="MRFDSLRPRLEPFLLRVERPGRYLGLERNVVRKDLSASELTLCL
                     AFPDAYEIGMSHTGTKILYELVNRRDGWTCERAYAPWVDLEEVMRREGIPLFSIESCA
                     PVADFDVLGFSLQSELNYTNVPNML"
BASE COUNT          304 a          768 c          779 g          263 t
ORIGIN      
        1 cgttcccgag accgccgaag atcgcgaggg agatcgtcga gatcgtcgag gccaccaggt
       61 cgcagtagcc gaggagctcg tggaaggtgg cgtaacgcgt cttcacgagg tcctgccggc
      121 agccggcgag gagcccctgg aacgccgcct tcgggatcgg gaagcgcgga agcgacccgg
      181 cgagcgcgac gcccgcggcg ctcgccgggc ttccgccgta gacggcgtcg agctcctcct
      241 cccacgcgtc gaggcgcgcg ggggcgcccg ccgggtcccc ctcgtcgacg gcgtcgtcgg
      301 cgacgcggca ccaggcatag gccgcgtaga cggcgtggcg ccgctccttc gggaggaagc
      361 ggaagccgac ggagaagttc gcgccggcct cgtgcgtgag cgcccggttc tcgcggcggg
      421 ccgcggagag ggcgggagcc ccgctcaacc gatcctcccg agcgcgacct cgcggatctc
      481 ccgctcgcgc ctccggcacc agtgctcggg aagcctgtgc gcgcagcgcc ctctgttccg
      541 ccagagggcc gccacgatcg gcgcgagggc gaggcggagc gcgcccggcc ggtagaagcc
      601 ggcgaggaga gcgcggaagt cggcgtgcgc ggcgaggcgc gcgaagagcg agagcccgaa
      661 ggcgcctccg gcctcgtaga ggaagcggtt cgcgacggag acgcgctggc ggcgggcgaa
      721 actcgcgcgc gcgagggccg cgaagtcgcc cccggcgagg agcgcctcgg ccgcgagagc
      781 gccggaggag atcgcgtagc gcatcccgag gccgaagagg tagtcctgga aggcgccggc
      841 ttcccccgcc gccgggcgcc cctcgcggag cggctctggc gcgagcgcga agttcatgaa
      901 cgagtaggcg tcgcgcgctc cctccatcgg gaccttctcg acggagagga gcttctccct
      961 cgccctctcg aagtagcggt cgatgccgcc gaggtcgcgg acgatcgcgc agccgaacgt
     1021 cccgcgccct ccgacggtga agaggtaggc gtagccgccg ggggcgaccg cggggtcgaa
     1081 gaggacccgg acgcgcgtcg cgccgtcgct ctcgaacgcc gtctcgcggg cgaggccgtc
     1141 gggcgtctgc ggccccgtcg ccacgaggtc ggcgtccttc ggctcgagcc gcgagcggaa
     1201 gcggacttcc gcgcccgcct cgagggcgcc gccgaggagc gtcgtgtcga gcgtccccgc
     1261 gtccggtccg cgcctcacga accaggcgta cggctccttc gaggagacca cggaccggcg
     1321 gagcttcgcg tcgaagaact cggccgtcgt cgccgggacc caggcgggcg ccgcaaggcc
     1381 gaggcgcgcg aacatcgccg ggacggtctc gcgcgggtcc gaggcgtcct cgatgacctg
     1441 caggtcgccg acgaatcggg cgccgaccgt ggcgttccgc tcgtggacga cgaccttccg
     1501 cccggcccgg gcgaggaggg tcgcggcggt caggccggcg ggaccggcgc cggcgatcga
     1561 gtacggaggc ggggcgctca cggggacggc cattattgcg gatcgagcgt cgggccctgc
     1621 gcgcggcgcg ccgctcctca cgacacgccc cgaatccgtc ggacgatcgg ccgaagagac
     1681 gccctcgcag ctctccggac taaactctgg ccctctcgga aaggcctcca gatgcgcttc
     1741 gattcccttc gcccccgact cgagcccttc ctcctccgcg tcgagcgtcc cggccgctac
     1801 ctcgggctgg agcggaacgt cgtccgtaag gacctctccg cctcggagct cacgctctgc
     1861 ctcgccttcc cggacgccta cgagatcggg atgagccaca cggggaccaa gatcctctac
     1921 gagctcgtca accgccgcga cggatggacc tgcgaacgcg cctacgcccc gtgggtcgac
     1981 ctcgaggagg tgatgcgccg cgaggggatc ccgctcttct cgatcgagtc gtgcgccccg
     2041 gtcgcggact tcgacgtcct cggcttctcc ctccagagcg agctgaacta cacgaacgtc
     2101 ccgaacatgc tcga
//
LOCUS       DAOTEZ010000594         2091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_45722_length_2091_cov_5.993615, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000594 DAOTEZ010000000
VERSION     DAOTEZ010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45722_length_2091_cov_5.993615"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="P5164_19810"
     CDS             <1..1013
                     /locus_tag="P5164_19810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019190155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRY46187.1"
                     /translation="EPDKSGRRRPVPTGEHETFACDTVIYALGTRANPVIARSAPDLG
                     VRGEGYVNADAKTQATNLPGVFAGGDIVTGGATVILALGAGRRAARAIETYLKTKEWP
                     VVLEDEAAGAGVPVPAPAAAACAKCRRPFEPGEEDHLCCGGEKVTWTCTSCQKVSEGF
                     AFPYGLCPACGGTLELGHDTEVTSEAAVAAIRHAFEIELGGVNFYSRGAKELEAKDPD
                     LAKLFRDLAEMERGHMDVLARRYHVEAPAEAFSESLSPAQVAVFAGADVKDFTGASLL
                     RLAVHLEKRARAFFLDAGRKFPTGSHEWRLYRELEAEEREHVDMLSTVLARIVAEKPV
                     VL"
     gene            1119..>2091
                     /locus_tag="P5164_19815"
     CDS             1119..>2091
                     /locus_tag="P5164_19815"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRY46188.1"
                     /translation="MRSRRLSAFGLLAALAVPAAAADGPRADGLRPPVVRLAWIEGDV
                     SIATGDAPGERRAGAVNDALTTDDRVRTGKGGRAELQLRGVVLFLAPGTDLAFPASAQ
                     DSRLLALSSGTMSIRVFGVRGVDDLAISTPNASVALELPGVYRFDVSGAGDTAISVAR
                     GRARVATADGGARLGWGDRMRVAGLVRPEYDVVDLGQGDAWDRWVERRSSRFRSVRSA
                     AHVHADVCGVDDLDTWGEWERTSERGWAWFPRVSGADWMPYRSGRFVWRDPWGWTWRS
                     DEPWGWAPYHHGRWTIVRDRWAWLPDGRQGPGPAWLPAVVAFVGSGPG"
BASE COUNT          246 a          800 c          761 g          284 t
ORIGIN      
        1 gcgagcccga caagtcgggc cggcgccgcc ccgtcccgac gggcgagcac gagacgttcg
       61 cctgcgacac ggtcatctac gcgctcggca cgcgcgccaa tcccgtcatc gcgcgctcgg
      121 cgccggacct cggcgtccgc ggcgaggggt acgtcaacgc cgacgcgaag acgcaggcga
      181 cgaacctccc cggcgtcttc gcgggaggcg acatcgtcac cggcggcgcg acggtcatcc
      241 tcgcgctcgg agcgggccga agggcggcgc gggccatcga gacctacctg aagacgaagg
      301 agtggcccgt cgtcctcgag gacgaggccg ccggcgccgg cgttcccgtc cccgcgccgg
      361 ccgcggccgc ctgcgcgaag tgccgccgtc ccttcgagcc gggcgaggag gaccacctct
      421 gctgcggcgg cgagaaggtc acctggacct gcacctcctg ccagaaggtc tccgagggct
      481 tcgccttccc gtacggcctc tgtcccgcct gcggcgggac gctcgagctc gggcacgaca
      541 ccgaggtcac ttcggaggcc gccgtcgcgg cgatccgcca cgccttcgag atcgagctcg
      601 gcggcgtcaa cttctactcg cgcggggcga aggagctcga ggcgaaggac cccgacctcg
      661 cgaagctctt ccgcgacctc gccgagatgg agcgcgggca catggacgtc ctcgcccggc
      721 gctaccacgt cgaggcgccc gcggaggcct tctccgagag cctctcgccg gcccaggtcg
      781 ccgtcttcgc cggggccgac gtgaaggact tcacgggggc ctcgctcctc cgcctcgcgg
      841 tccacctcga gaagagggcc cgcgccttct tcctcgacgc cggccggaag ttcccgaccg
      901 gctcgcacga gtggcgcctc taccgcgagc tcgaggccga ggagcgcgag cacgtcgaca
      961 tgctctcgac cgtcctcgcg cggatcgtcg cggagaagcc ggtcgtcctg tagcgccacg
     1021 gtcgcggccg ccccggaggg gtccccctcc ggggcggccg ccgcagtatc ccgtcgtcac
     1081 cggcgccgtt tcgaacgatc atgctccggg aggtctccgt gcgctctcga cgcctctctg
     1141 ccttcggtct gctggccgcc ctcgccgttc cggccgcggc tgcggacgga ccccgcgccg
     1201 acggcctccg gccgcccgtc gtgcgcctcg cctggatcga gggcgacgtc tcgatcgcga
     1261 cgggcgacgc cccgggcgag cgccgggccg gggcggtcaa cgacgctctg accacggacg
     1321 accgtgttcg caccgggaag gggggccgcg cggagctgca gctccgcggc gtcgtcctct
     1381 tcctcgcgcc cgggacggac ctcgcgttcc cggcgtccgc gcaggactcc cgcctcctcg
     1441 ccctctcgtc ggggacgatg tcgatccgcg tcttcggcgt gcgcggcgtg gacgacctcg
     1501 cgatctcgac gccgaacgcg tccgtcgccc tcgagctccc cggggtctac cgtttcgacg
     1561 tgagcggagc gggtgacacg gcgatcagcg tcgcgcgcgg ccgggcccgc gtcgcgaccg
     1621 cggacggagg agcccggctc ggctggggcg atcggatgcg cgtcgccggc ctcgtccggc
     1681 ccgagtacga cgtcgtcgac ctcggccagg gggacgcctg ggaccgctgg gtcgagaggc
     1741 gctcctcccg cttccggtcc gtccggtccg ccgctcacgt gcacgccgac gtctgcggcg
     1801 tcgacgacct cgacacctgg ggcgagtggg agagaacgtc cgagcggggg tgggcctggt
     1861 tcccgagggt gagcggcgcg gactggatgc cgtaccgctc gggccgcttc gtctggcgcg
     1921 acccgtgggg ctggacctgg cgctcggacg agccctgggg ctgggccccg taccaccacg
     1981 gccgatggac gatcgtccgt gaccgctggg cctggctccc ggacggacgg cagggccccg
     2041 gcccggcctg gcttcccgcc gtcgtcgcgt tcgtcggaag cgggccggga g
//
LOCUS       DAOTEZ010000595         2090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_45757_length_2090_cov_5.275184, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000595 DAOTEZ010000000
VERSION     DAOTEZ010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45757_length_2090_cov_5.275184"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..742
                     /locus_tag="P5164_19820"
     CDS             107..742
                     /locus_tag="P5164_19820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46189.1"
                     /translation="MTAAVASLVGTALLLAAAFRGGAGLVRGTAPGRRPSLLVLAFAT
                     AFSIVPVAGHPVHRWLATFYPILGVPLAALLFDVTWSGTTGRPVFRDADRRAFWAFGS
                     AAGLFLYPMSLGLGPFDPYALGWSFSWLFPSIGAVAAVLILRGNRFGYALLLALAAWH
                     GGLLESTNLWDYLVDPAYFLMSVGALSGRAFRHAAPAAPLSPSRFARRAPE"
     gene            complement(705..1937)
                     /locus_tag="P5164_19825"
     CDS             complement(705..1937)
                     /locus_tag="P5164_19825"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRY46190.1"
                     /translation="MELSRKVRGTLDLGETLDRLLDLVRSIVEYDAAGIFVLNEDVLA
                     GERRRGMIAGVTQRGFPARPLEADPMLFRGEGITGHVIRTGECVVASDVRLDSRYVVG
                     RASTLAEIAVPITVEGRSIGALNLESDRLGAFDEQHLDALRFFAEAAAVAIDKAMLHR
                     GLLEKQKLEAQLRVAHQVQSRLLPQAPPALDGWDVAAVYIPSSELAGDYYDFLRLPDG
                     RVGLVIADVAGKGVPAALIMATFRALLRAHVASGAGVAEIATRLNGLLRDECRPHGFV
                     TGFLAFLEPSTGRLEYANCGHVPPLVVAPAGEPVELAEGGPVLGVFAEAAYGSREVTL
                     APGQVLVLHTDGVSEARRAEGEELGSARLAEVVRAGLSRGATSLAGEVVRVARAFTGT
                     EAFEDDFTLVLVARIAKG"
BASE COUNT          260 a          788 c          724 g          318 t
ORIGIN      
        1 tccgcgcgcg cgacggccgc ctgcacctcg tctactcgtg gcagatgaag cgcatccggc
       61 acgtcacgtt caacgaggcg tggctgcgcg cgcgggaagc cgcgagatga cggctgccgt
      121 cgcctcgctc gtcggcaccg cgcttctcct ggccgccgcc tttcggggag gggcgggcct
      181 cgtccggggg accgctcccg gccgacggcc atcgctcctc gtcctcgcct tcgcgaccgc
      241 cttttcgatc gtgcccgtcg ccgggcatcc ggtccaccgg tggcttgcga ccttctatcc
      301 gatcctcggc gtgcccctgg ccgcgctcct gttcgacgtc acctggagcg gcacgacggg
      361 gcgccccgtg ttccgcgatg ccgaccgcag ggcgttctgg gcgttcggat cggccgccgg
      421 gctcttcctc tatccgatgt cgctcggcct cggccctttc gatccgtatg cgctcgggtg
      481 gagcttctcg tggctgttcc cctcgatcgg ggccgtcgcg gcggtcctga tcctgcgcgg
      541 caaccgcttc ggctacgcgc tgctcctggc cctcgccgcc tggcacggcg gcctcctcga
      601 gtcgacgaac ctctgggact acctggtcga ccccgcatat ttcctcatga gcgtcggcgc
      661 cctttcgggc cgcgcattcc ggcacgcggc tccggccgct cccctcagcc cttcgcgatt
      721 cgcgcgacga gcaccagagt gaagtcgtcc tcgaaggcct cggtgcccgt gaatgcgcgt
      781 gccacgcgga cgacttctcc ggccagggag gtcgcgcccc gcgagagtcc cgcacggacg
      841 acctcggcga gcctcgcgga gccgagctcc tcgccctccg cccggcgggc ctcgctgacg
      901 ccgtccgtgt ggagcacgag gacctgcccg ggggcgagcg ttacctcacg cgagccgtag
      961 gccgcctccg cgaagacccc cagcacgggg cctccctccg cgagctcgac cggctcgccc
     1021 gccggcgcga cgacgagcgg cggcacgtgg ccgcagttcg cgtactcgag ccttcccgtc
     1081 gagggctcga ggaaggcgag gaaccccgtc acgaagccgt gcggacggca ctcgtcgcgc
     1141 aggaggccgt tcagccgcgt cgcgatctcc gcgacgccgg cccccgacgc cacgtgagcc
     1201 cggaggagcg cgcggaaggt cgccatgatg agagccgccg gcaccccctt gccggcgacg
     1261 tcggcgatca cgaggccgac ccggccgtcc ggcaggcgga ggaagtcgta gtagtcgccc
     1321 gccagctccg agctcgggat gtagaccgcg gcgacgtccc agccatcgag ggccggcggc
     1381 gcctgcggga ggagccgcga ctggacctgg tgagcgaccc tcagctgcgc ttcgagcttc
     1441 tgcttctcga ggagtccccg gtggagcatc gccttgtcga tcgcgacggc cgccgcctcg
     1501 gcgaagaatc ggagcgcgtc gaggtgctgc tcgtcgaagg ccccgaggcg atcgctctcg
     1561 aggttgaggg cgccgatcga cctcccttcg acggtgatcg gcacggcgat ctcggcgagg
     1621 gtcgacgcgc ggcctacgac gtaccgggag tcgaggcgca cgtcggacgc cacgacgcac
     1681 tcccccgtcc ggatcacgtg gccggtgatc ccctcgcccc ggaagagcat cgggtcggcc
     1741 tcgagcggcc gcgcggggaa accgcgctgc gtgacgcccg cgatcatccc ccgccgacgc
     1801 tcgccggcga ggacgtcctc gttcaggacg aagatccccg ccgcgtcgta ctcgacgatg
     1861 gagcggacca ggtcgaggag ccggtcgagc gtctcgccga ggtcgagcgt gccgcggacc
     1921 ttcctcgaga gctccaggag gagaccgtac cgcgcttcgg ccgtgagccc ctcggcgtgc
     1981 tcggggcgat cgggcgggcg tcttctcggt tcatcggcac ctcgggacgg cgcgggtcga
     2041 tgctaacagc ccgctcacga cgtctcccgg ttcgccgcgg gcgtacactt
//
LOCUS       DAOTEZ010000596         2074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_46331_length_2074_cov_8.124814, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000596 DAOTEZ010000000
VERSION     DAOTEZ010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46331_length_2074_cov_8.124814"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="P5164_19830"
     CDS             complement(<1..629)
                     /locus_tag="P5164_19830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRY46191.1"
                     /translation="MTRKRPDGAGQPALAFDAEIALPEEARRRYLNYALSVITARALP
                     DVRDGLKPVQRRILFAMYQNLHLTPDAKFKKSASVVGDVIGKYHPHGDVAIYDAMVRM
                     AQPFSLRSPLVEGHGNFGSLDGDSAAAYRYTEARLRPLAVELLSEIRKRTVDWKPNFD
                     GVHFEPVVLPARFPNLLVNGATGIAVGMATSIPPHNPCEVLDAAIAAID"
     gene            complement(716..>2074)
                     /locus_tag="P5164_19835"
     CDS             complement(716..>2074)
                     /locus_tag="P5164_19835"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRY46192.1"
                     /translation="IAGITPDVVLVSIGAKSEALMDLHELEGEKVGDRIDAVVIKAGP
                     DIRLSRKLAVARRAKAELRAAWETGIPVLGKVLSRNKGGFEVAIGGPGGPRAFCPVSQ
                     IDVGRHDEPGLLQFVGQSFDFRVIEYAEDGRRVVVSRAAILREALEKSLGEIRERIVP
                     GAVLTGHVRSITDFGAFVDLGGVDGLVHVTEISRRRVAHAKDVLAIGQEVQVKVTKVE
                     NEGKRISLSMKELEKDPWEGLAQRIPPGTPFTKPVARHAEFGLFIEIEPGIDGLVHVS
                     QLPPGIDLKDPSVAAGQEVSGWVREIDEEHRRLSLSLREASATDPWDGLAEKMPEGSV
                     VPGQVENVAPFGVFVRLEIGLTGLIPNSETGLPHGTPASKTFSPGQKVEVKVIGLDAQ
                     RRRISLSATGALAEAERGEIRRYREDSAKRERSEPGRPTNFGASLMAALSSPNKPAKS
                     AR"
BASE COUNT          284 a          713 c          771 g          306 t
ORIGIN      
        1 tcgtcgatgg cggcgatcgc cgcgtcgagg acctcgcacg ggttgtgcgg cgggatcgag
       61 gtcgccattc cgaccgcgat gcccgtcgcg ccgttgacga ggaggttcgg gaagcgggcc
      121 ggcaggacga ccggctcgaa gtggaccccg tcgaagttcg gcttccagtc gacggtccgc
      181 ttccggatct ccgaaaggag ctcgaccgcg agcgggcgga ggcgcgcctc ggtgtaccgg
      241 taggccgcgg cggagtcccc gtcgagcgag ccgaagttcc cgtgcccctc cacgagcggc
      301 gagcggagcg agaacggctg cgccatcctc accatcgcgt cgtagatcgc cacgtcgccg
      361 tgcgggtggt acttgccgat cacgtcgccg acgaccgacg ccgacttctt gaacttcgcg
      421 tcgggcgtca ggtggaggtt ctggtacatc gcgaagagga tccgccgctg gaccggcttc
      481 aggccgtccc tcacgtcggg gagggcccgc gccgtgatga ccgagagggc gtagttcagg
      541 tagcgccggc gggcctcctc ggggagggcg atctcggcgt cgaaggcgag cgccggctgg
      601 ccggcgccgt cggggcgttt ccgcgtcatg agtgcctcaa aagagaatcc ccccggcccg
      661 cggggccggg gggacggatg ttacggtctg ccgcggcccc gatggggccg ccccctcacc
      721 gcgccgactt ggccggcttg ttcggagagc tgagcgccgc catcagggag gcgccgaagt
      781 tcgtcggacg gcccggctcg ctcctctcgc gcttggcgga gtcctcgcgg tagcgccgga
      841 tctcgccccg ctcggcctcg gcgagggcgc cggtggcgga gagggagatg cggcgccgct
      901 gggcgtcgag gccgatcacc ttcacctcga ccttctggcc gggcgagaac gtcttcgagg
      961 ccggcgtccc gtgcgggagc cccgtctcgg agttcgggat gaggccggtg aggccgatct
     1021 cgaggcggac gaagacgccg aacggagcga cgttctccac ctggccgggg acgaccgagc
     1081 cctccggcat cttctcggcg agcccgtccc acgggtcggt cgcggaggcc tcccgcaggg
     1141 agagggagag ccggcggtgc tcctcgtcga tctcgcggac ccagccggag acctcctggc
     1201 cggccgcaac ggacgggtcc ttcaggtcga tgccgggcgg gagctgggag acgtggacga
     1261 gcccgtcgat gccgggctcg atctcgatga agaggccgaa ctcggcgtgg cgcgccacgg
     1321 gcttcgtgaa gggcgtcccg ggcgggatcc tctgggcgag gccctcccac gggtccttct
     1381 cgagctcctt catggaaagg gagatccgct tcccttcgtt ctccaccttc gtgaccttga
     1441 cctggacctc ctggccgatc gccaggacgt ccttcgcgtg ggccacgcgg cggcgggaga
     1501 tctccgtgac gtggacgagg ccgtcgacgc cgccgagatc gacgaaggcg ccgaagtcgg
     1561 tgatcgagcg gacgtggccc gtcaggacgg cgccggggac gatcctctcg cggatctcgc
     1621 cgaggctctt ctcgagcgcc tcgcgcagga tcgccgcccg gctgacgacg acccgccggc
     1681 cgtcctcggc gtactcgatg acgcggaagt cgaacgactg gccgacgaac tggaggaggc
     1741 cgggctcgtc gtgacggccg acgtcgatct gcgagacggg gcagaaggcg cgcggcccgc
     1801 cggggccgcc gatggcgacc tcgaacccgc ccttgttgcg ggacaggacc ttgccgagga
     1861 ccgggatccc ggtctcccac gcggcacgca gctccgcctt cgcgcggcgc gcgacggcga
     1921 gcttcctcga gagccggatg tcgggcccgg ccttgatgac cacggcgtcg atccggtcgc
     1981 cgaccttctc gccctccagc tcgtggaggt ccatcagcgc ctcggacttg gccccgatcg
     2041 agacgaggac cacgtccggc gtgatgccgg cgat
//
LOCUS       DAOTEZ010000597         2065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_46676_length_2065_cov_6.483582, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000597 DAOTEZ010000000
VERSION     DAOTEZ010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46676_length_2065_cov_6.483582"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..2065
                     /locus_tag="P5164_19840"
                     /pseudo
     CDS             170..2065
                     /locus_tag="P5164_19840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
BASE COUNT          280 a          752 c          778 g          255 t
ORIGIN      
        1 acccactcag gtgccaccca ctcaggtgcc aggcgtgaaa tcgtgtattt ctacagaaat
       61 acacgatttc acgcctggca cccatccggg gggcaccgac ccggggggag gggccgcgag
      121 aggagcacga ggccggagag ggccggtgac ggtcgagagg ctcggcgagc tcctgacgga
      181 ggtcttcggg cacgagtcct tccggccgca ccaggaggcc gtctgccggg cggtcgtcga
      241 ggggcgcgac gggctcctcg tgatgccgac gggggcgggg aagtcgctct gctaccagct
      301 gccggggctc gcgcgcggag ggacgacgct cgtcgtgtcg cctctcatcg cgctcatgga
      361 ggaccaggtc gcgaagatgc aggcgctcgg cctcgccgcc gagcggatcc actcggggcg
      421 cgaccggggc gcttcgcggc aggtctgcct cgactacctc gccgggcgcc tcgacttcct
      481 cttcatcgct cccgagcggc tgtcggtacc gggcttcccg gagatgctcg cgagacggac
      541 gccggccctc gtcgcggtcg atgaagcgca ctgcatctcg cactgggggc acgacttccg
      601 tccggactac cggctcctcg gcgcgcgcct cccgcagctg cggcccgccc ccgtcctcgc
      661 cctcaccgcc accgcgacgc cgcgggtcca ggaagacatc gccgcgcagc tcgggctcgt
      721 gaagccgggg cgcttcatcc acggcttccg ccggacgaac ctcgcggtcg aggcggcgga
      781 ggtgccgccg agccggcgcg gcgcgctcgt ggcggagctc ctcgccgacc cggcgcggcg
      841 gccggcgatc gtctacacgc cgacgcggaa ggaggcgacg gcgctcgcgt cggagctgaa
      901 gggggaggtc cgcgccgtcg cctaccacgc cgggatgacg gcgcgggacc gcgacgacgt
      961 gcaggcgcgc ttcctcgcgg ggcgcgcgga ggtgatcgtc gcgacgatcg cgttcgggat
     1021 ggggatcgac aagccggacg tgcggaccgt gatccacacc ggcctccccg ggagcgtcga
     1081 gggctattac caggagatcg ggcgcgcggg gcgcgacggg ctgccgtcgc gcgcgatcct
     1141 cctccactcc tggggcgaca tccggacgca cgagttcttc ctcgagcggg actacccgga
     1201 ggcgcacgtc ctcggcgacg tcttccgcgc gctgaaggaa gcgccggttc cgacggaggg
     1261 cctcgcccag ctcgtcccgc tcgagcccga cgtcttccag aaggcgctcg agaagctctg
     1321 ggtcttcgga ggcgcggtca tcaccccgga aggggacgtc tcgcgcggga aggacggatg
     1381 gcagcgggcc tacgacgagc agcgagagca ccgcaagacg cagctcgtcc tcatgcgccg
     1441 cttcgccgag gggccgatct gccggatgct ccagctcgtc cgccacttcg gcgacgagga
     1501 ggacgacgga gcgccgtgcg gcctctgcga cgtctgcgcc ccgggcgacg gcctcgccgc
     1561 cgacacccgg aggacgacgc cgatggagga ggccgcgctc cgccgcgccc tcgagctcct
     1621 ccggccgcgc gacgggcaga cgacggggca gctccacgcg gaggtcgtcc ggcagttccc
     1681 ggcggtcgac cggcgcgcct tcgaggagct cctcggcggc ctcgtgcggg ccggcctcgc
     1741 gcggctcgag gacgacgcgt tcgagaagga ggggcgcgtc atccgcttcc gccgcgtctt
     1801 cctgacgggc gcggggagcc gtcccggcgc ggccgtcgag gcccgcgtcg cggtgccgcc
     1861 accgagcacg acgaaggcga ggaaggcgaa gggcgcgggg ccgaagcggg agaggctccc
     1921 gaagctccct atcccgaagc cgtcggcggg gcgtccctcg ctcccccacg ggggcgcgcg
     1981 aggaaagccc ggccccgagg cgccgggcgc cctcccggcg ctcgtcgacg ccctccgccg
     2041 ctggcgcctc ggcgaggcga agaag
//
LOCUS       DAOTEZ010000598         2060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_46875_length_2060_cov_5.286783, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000598 DAOTEZ010000000
VERSION     DAOTEZ010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46875_length_2060_cov_5.286783"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="P5164_19845"
     CDS             <1..353
                     /locus_tag="P5164_19845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HRY46193.1"
                     /translation="ATTVKLTRALWIVPCVLGAALLARSGERPKFPLFIAGFVAAAAL
                     RTVAPQLEPLWTGLAAVARQLLVLTLFLIGTGLTREVLVRVGLRPLAQGLFLWLGVGS
                     ATLAAILSGLIPPL"
     gene            1860..>2060
                     /locus_tag="P5164_19850"
     CDS             1860..>2060
                     /locus_tag="P5164_19850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46194.1"
                     /translation="MAVRFEPAQDAKGGVAFPGGELQVPVERGSWEAVIPEGRFDLVL
                     RSRGFGARYLFGVAVGPGTPRNL"
BASE COUNT          295 a          668 c          757 g          340 t
ORIGIN      
        1 tcgccacgac ggtgaagctg acgcgggcgc tctggatcgt gccgtgcgtc ctcggtgcgg
       61 cgctcctcgc ccgctcgggc gagaggccga agttcccact cttcatcgcg ggcttcgtcg
      121 ctgcggctgc cctccggacc gtcgctccgc agctcgagcc gctctggacc gggctcgcgg
      181 cggtggcgcg gcagctcctc gtcctcaccc tcttcctgat cgggaccggc ctgacgcggg
      241 aggtcctggt gcgggtcggc ctccgcccgc tcgcccaggg tctcttcctc tggctcggcg
      301 tcggctcggc gacgctcgcc gcgattctct cgggcctgat tccgccgctc tgaaaggggt
      361 ccggaccgcg cctccttcgg tgttcacggc ggcgtcgcga gcgaacggcg cgcgaaatgg
      421 ccggatacgc caggtcgaac cggggaggag cgagctgccg cgagcggttc gtgaactttt
      481 tcttcggagg aggggcaatt cggggggtct gaatccctca ttagtagtag gagggcctct
      541 cttgctcccg aattccgaga tctgttctct atccgatgtc ggattcctgc cggtcgggag
      601 gcctggccgg gatggccccg atcgggtcct cgagggctcg ggcttcgaac gactcgccgc
      661 gcggctcgcc caggcgggga cgaaggccgc cgcagtcgag gccggggtgt ccgtccgaac
      721 ccttcatcgg aggctcgcga aacgagggac gatggcgcag gcgctcgttc gcaggttccg
      781 gagggaacga gctctccgcc tcctcggcga tgcggccagg ctcgacgcgg tcgccgaggc
      841 gctcggcttc tccggtccga gcgccctcgc ccgcttctgc aggaaggagc tcggcaggac
      901 cgccggcgag ctgcgggagg aactggtccg gaagggccgg cttccgtggg tacgatgcac
      961 gaaatgtggc acgaaatgtc aatagacgag accggcgcgg agcgcggatc atccgtggaa
     1021 gatcgatggt ccgcgggaca acgaggagga gtcggcgaca gtgggggaac aggaagcggc
     1081 tccccatcgc gcctctcgct caccaggaag cccgtcgagg acgagaaacc gaaggaggtt
     1141 cgggatgaag cgcctcatcg tggtggtcgc gatatcactc ggaattctct ggagcccatc
     1201 cgcatgggcg gcacgggacc ccatgtgcca ggacgagaac ggacagacga tcccatgtga
     1261 gaaggacggc ggcggaggtc tcgctctccc cggtcccccc tgctcgcatc ccacctgctg
     1321 gggttgcggc gccgaacagt ccggcggata ccggccgtgt gtgccgtacg ccggcctcgc
     1381 ctgcaactgc ttgtacgacg acaacggcgg caagaatccg gatcacaagt gctacccgta
     1441 cggcccgtgc acgttcacgg gaccgtaggc ccgtgaaggc aacggggaac ctcgtgagag
     1501 caactcttct tgcacttctg accgcgggtc ttgccccgat gctcgcgacc gcctcgccgg
     1561 tgcgggtcgt cgtgggccct ggcgggccga tcgacctccc gcgcggctcc gaggtcgtcg
     1621 cgatcgggcc ggcgggagtc ctcgcgcgcg cgccggttgt ttcgtgggag gaggaggtcc
     1681 ggttcgatgt ccccgaggga gcggaccgga tctccgctga ggccgaaggg atgtggtgca
     1741 gctcgacgcc attcgaagag cgtgctccgg ccgacgcagc gaggagagtc tcctgctgga
     1801 ggtccgggaa gatcaggggc acggtcgcct ccgcgacgcg ggggggcgag gtgaccgcgt
     1861 tggcggttcg cttcgagccc gcgcaagatg cgaagggcgg ggtcgctttt cccggcggcg
     1921 agctccaggt gcccgtcgag cgggggagct gggaggccgt gattcccgag ggtcgcttcg
     1981 acctcgtcct gcgcagccgt ggattcggcg cgcgatacct gttcggcgtg gcggtgggtc
     2041 cggggacgcc gagaaacctc
//
LOCUS       DAOTEZ010000599         2050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_47280_length_2050_cov_3.434085, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000599 DAOTEZ010000000
VERSION     DAOTEZ010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47280_length_2050_cov_3.434085"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            339..1592
                     /locus_tag="P5164_19855"
     CDS             339..1592
                     /locus_tag="P5164_19855"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRY46195.1"
                     /translation="MGIRSHGRWMLALAGLVGTLEAHADDGLRMDDYVRLVLQSHPAA
                     GAAAALDASASAESKAARLLPDPTFEVSLGKGQASDGSGSAKTEKGFSVSQSIPWLPA
                     RSASIEAADHEARALRAEGLATRWGLELDARVAYARLLFARAQVEVSRASTADAASLL
                     DLMTRRESLGEAREVDRIKSRVEWLRQDRELSAAYREELAAEGILRTLAGAPLPDPLR
                     LEGELPRHTDAMRALLDAKPENLERSPALIRARAEASRASSLLSSARRGRIPDLGFSF
                     FRQNELDREAWGGALGLSLPLWNARRGDVARAEAESRLRAADAERVRLSLFADLATRR
                     RDVETLAVQVASLEGDLLPSAAESVRLARLLFEEGETSLLDLLDAQRTAREARREEIR
                     ARFELMTAIAELQRLLGPNDAPTGR"
     gene            1596..>2050
                     /locus_tag="P5164_19860"
     CDS             1596..>2050
                     /locus_tag="P5164_19860"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY46196.1"
                     /translation="MTKTLTASWLLAAILGLTLALSGCGKTKPAPGKGEEAGHPAESG
                     EETHAAEIPDEVRITAAAISEAGIATWKVQVVDLAHLLSLNGTVGYDENRLLQVAANV
                     KGRIASIPVDLGSPVAKGQSLLVIESVDLGRAREEFVRELSAFNVSARA"
BASE COUNT          293 a          777 c          692 g          288 t
ORIGIN      
        1 cgcaagtccc gccccgcgcc cgtgccgccc ctcgttcgag gcgaccgtgc ctcctgcctc
       61 ccggcacatc ggcgagatcc tgcacgtccc gaaaccggcc ctcgcgtagc agcggctgag
      121 ggaggcccca tccggaagcg tcgcgcaaag agcgacgctc gccggaggcg gcattccctc
      181 ggacctcttc gctgacgggt cggccatccc ggccggcacg ccgccaccgc ggcgggtcga
      241 cgcacgcgaa tcccgggcgg attcactcga atcccgacct gggccctcgg tcaagaccgg
      301 ggagcgcgct tctctcccgc cgcttccagg aggacggaat gggaattcga tcgcatggcc
      361 gctggatgct cgcgctcgca ggtctggtcg gcactctcga agcgcacgcg gacgacgggc
      421 tccgaatgga cgactacgtc cggctcgtcc tgcagtcaca cccggccgcg ggggcggcgg
      481 cggcgctcga cgcgagcgcc tcggcagagt cgaaggcggc acgtctcctc cctgacccga
      541 cgttcgaggt gagcctcggg aaggggcagg cctcggacgg ctccggctcc gcgaagacgg
      601 agaagggctt ctccgtctcg cagtcgatcc cgtggcttcc ggcccgttca gcgtcgatcg
      661 aggccgcgga tcacgaggcc agggcgctgc gtgccgaggg actcgcgacg cggtggggcc
      721 tcgagctcga cgcgcgagtc gcgtacgccc gtctcctctt cgcccgggcg caggtcgagg
      781 tgagccgggc ctccacggcg gacgctgcgt ccctcctcga cctgatgacc cggcgtgaat
      841 cgctgggcga ggcgcgcgag gtggaccgga tcaagtcgcg cgtcgagtgg ctccggcagg
      901 atcgtgagct gagcgccgcc tacagggaag agctcgccgc ggaaggcatc ctgcggacgc
      961 tcgcgggagc gccgctcccc gatccgctac gcctcgaggg agagctgccg cggcacaccg
     1021 acgccatgcg cgccctcctc gacgcgaaac ccgagaacct cgagagaagc ccggcgctga
     1081 tccgtgcccg cgccgaggct tcccgcgcct cgtcgctcct ctcgagcgcc cgccgaggtc
     1141 ggatcccgga cctcggcttc tccttcttcc ggcagaacga gctcgatcgc gaggcatggg
     1201 gcggcgcgct cggcctctcc ctgccgctct ggaacgcacg gcgtggagac gtcgcgcgcg
     1261 ccgaggccga gagccgcctc cgggccgccg atgccgagcg cgtgcgcctt tctctcttcg
     1321 ccgacctcgc gacacgccgc cgcgacgtcg aaaccctggc cgtccaggtt gcctcgctcg
     1381 agggagacct ccttccctcc gctgccgaga gcgtccgtct cgcccgcctc ctcttcgagg
     1441 aaggcgagac ctccctcctc gacctcctcg acgcacagcg cacggcccgc gaggcccgcc
     1501 gcgaggagat ccgcgcccgc ttcgagctga tgaccgcgat cgccgagctt cagcggctcc
     1561 tcggtcccaa cgacgccccc accgggagat agacgatgac gaagactctc actgcctcat
     1621 ggcttctcgc cgcgatcctc ggcctcaccc tcgcgctctc cgggtgcggg aagacgaagc
     1681 cggctcccgg gaagggggaa gaggccgggc accctgcgga atccggagag gagacccacg
     1741 ccgccgagat tcctgacgag gtccggatca ccgccgccgc catctccgag gcgggaatcg
     1801 cgacctggaa ggtccaggtc gtcgacctcg cgcatctcct ctccctcaac gggacggtcg
     1861 gctacgacga gaaccgcctc ctgcaggtcg cggcgaacgt gaaggggcgc atcgcgtcga
     1921 tcccggtcga cctcggctcg ccggtcgcca agggccagtc gctcctcgtc atcgagagcg
     1981 tcgacctggg ccgggcgcgc gaggagttcg tgcgggagct ctccgcattc aacgtctcgg
     2041 cgcgcgcctt
//
LOCUS       DAOTEZ010000600         2046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_47433_length_2046_cov_3.883476, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000600 DAOTEZ010000000
VERSION     DAOTEZ010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47433_length_2046_cov_3.883476"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..525
                     /locus_tag="P5164_19865"
     CDS             82..525
                     /locus_tag="P5164_19865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006853791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRY46197.1"
                     /translation="MSRVALLDVNVLVALFDPDHVHHEAAHAWFAPNRGVGWATCPIT
                     ENGVVRVLSNPAYGAEPERPGALLARLRSFCASGGHVFWPDDVSLRSARALRAGAALS
                     HGTLTDVYLLALAVAHGGRLATFDRRIPVAAVAGAEKEHLELIPA"
     gene            complement(805..>2046)
                     /locus_tag="P5164_19870"
     CDS             complement(805..>2046)
                     /locus_tag="P5164_19870"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY46198.1"
                     /translation="QHEDCVDLTPGQVQELNRYSKLVLFHSAGGQPDVFYTSPDVASV
                     PGARELAARPELLEESPGYRMFVEGHDQVRVYSVPYRSRAGRRGVIRVMERMGDVELP
                     LRNLRLGLLFLAPAAIALAALVTWSLARRALAPVVEVTALAREIEATQLSRRLPAPAA
                     PDEIGRLVETFNQMIARLESSFEAMKRFTADASHELRSPLANVKSTVELALGRPRSDE
                     EYRSALASVGEEAERLRRIVEDLLLLARADSGRLPFEKEEVRLDVLAREVVESFGPRA
                     LEAGLRVEAVAESDVVVLGDERWLRQLVQNLVENAVQFSPARGSGKPPALVAIGVKAD
                     GEDALLVVEDEGPGIPEAERDRIFERFYRVDASRQRGAGTGAGLGLAICSWIVDAHRG
                     RIDASGRADGGTAFRVRLPRA"
BASE COUNT          281 a          754 c          721 g          290 t
ORIGIN      
        1 cggggtcccc cttctcccgc gaaggaagaa ggggagcccg cgcccgacga tggcgctcgt
       61 caaccgtctg cgggacgaag ggtgagccgg gtcgcgctcc tcgacgtcaa cgtcctcgtc
      121 gccctcttcg atccggacca cgtccaccac gaggccgcgc atgcctggtt cgcgccgaat
      181 cgcggcgtcg gctgggcgac ctgcccgatt acggagaacg gggtcgtccg ggtcttgtcg
      241 aacccggcgt acggcgccga gccggagcgc ccgggtgcgc tcctcgctcg cctccggtcg
      301 ttctgcgctt cgggcgggca cgtcttctgg cccgacgacg tctcgctccg gagcgcgcga
      361 gcgctccgag cgggcgcggc gctgtcgcac ggcacgctga cggacgtcta tctcctcgcg
      421 ctcgccgtgg cgcacggcgg gaggctcgcg acgttcgacc ggcggatccc cgtcgcggcc
      481 gtcgcgggag cggagaagga gcacctggag ctgattccgg cctgatcggg ccggcgcccg
      541 tccgagcccc tccgctagtc tcccccccgc gcttccgcgc tcttcatgga catcgtcgcc
      601 agcgatgccc gctggctcgg gaaagaggag gggaacgggc caacaagaac ccgtttctga
      661 caggcctgcg ccggcgcggt cgcttcctgg tggaggccgc gcagcactca gacacgacga
      721 agatcgtgcg gacgtgtcgc tggtcggcgg agggagctgc ccgacggctc cctccatttc
      781 gcatctctgc gatttgccag tcgctcaggc gcgaggaagc cgaacgcgga aggccgtccc
      841 gccgtcggct cgcccggagg cgtcgatccg tccccggtgc gcgtccacga tccacgagca
      901 gatggcgagt ccgaggccgg cccccgttcc ggcgcctcgt tgccgggagg cgtcgacgcg
      961 gtagaagcgc tcgaagatcc gatcccgctc cgcctcggga attccgggtc cctcgtcctc
     1021 gacgacgaga agcgcgtcct cgccgtcggc cttcacgccg atcgcgacga gggccggcgg
     1081 cttcccggac ccgcgcgccg gcgagaactg gacggcgttc tccacgaggt tctgtacgag
     1141 ctgtctcagc cagcgttcgt cgcccaggac gacgacgtcg gattccgcga ccgcctcgac
     1201 cctgagcccg gcctcgagag ctcgcggccc gaacgactcc acgacctccc gggcgaggac
     1261 gtcgagccgg acctcctcct tctcgaacgg gagccggccg gagtcggccc gggccaggag
     1321 gagcaggtcc tcgacgatcc ggcggagccg ctcggcctcc tcgcccacgc tcgcgagcgc
     1381 ggaacggtac tcctcgtccg agcgcggccg tccgagcgcg agctcgacgg tgctcttcac
     1441 gttcgcgagc gggctgcgaa gttcgtgcga ggcgtcggcg gtgaatcgct tcatcgcctc
     1501 gaacgacgac tcgagcctcg cgatcatctg gttgaacgtc tcgacgagac ggccgatctc
     1561 gtccggcgcg gccggggcgg ggagccgccg gctgagctgc gtcgcctcga tctcacgcgc
     1621 gagcgcggtc acctcgacga cgggggcgag cgcgcgtcgg gcgagcgacc acgtcacgag
     1681 cgccgcgagc gcgatcgccg cgggagcgag gaagaggagc cccagccgga ggttccggag
     1741 cggcagctcc acgtcgccca tccgctccat cacccggatc accccgcggc gtcccgcccg
     1801 cgagcggtac gggacggagt agacgcggac ctggtcgtgc ccctccacga acatccggta
     1861 gcccgggctc tcctcgagga gctccggccg cgccgcgagc tccctcgcgc cggggaccga
     1921 ggcgacgtcc ggggacgtgt agaagacgtc gggctgcccg ccggccgagt ggaagaggac
     1981 gagcttcgaa taccggttca gctcctgcac ctgcccgggc gtcaggtcca cgcagtcctc
     2041 gtgctg
//
LOCUS       DAOTEZ010000601         2043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_47542_length_2043_cov_4.928571, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000601 DAOTEZ010000000
VERSION     DAOTEZ010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47542_length_2043_cov_4.928571"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1059)
                     /gene="recA"
                     /locus_tag="P5164_19875"
     CDS             complement(7..1059)
                     /gene="recA"
                     /locus_tag="P5164_19875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRY46199.1"
                     /translation="MPPIEKEKLKALEQALQQIDRQFGKGALVRLGDKPQEAIQAIST
                     GSINLDAALGIGGVPRGRVTEIYGPESSGKTTLTLHIIAEAQRLGGLAAFIDAEHALD
                     AEYARKLGVDVDNLMVSQPDSGEQALEIAEALVRSAAVDIVVVDSVAALVPKAELEGE
                     MGDAMVGLQARLMSQALRKLTAVVSKSGTCLIFINQIREKIGVMFGNPETTTGGRALK
                     FYSSVRLDIRRINSIKDGDAVVGSRTKVKVVKNKVAPPFKVAEFDIGYGEGISKTGEL
                     IDLGVEHKVVEKSGAWFSYGDLRIGQGRENTKAWLRDNPDVALEIENKIREKLGISPV
                     EPPAPPAAAAPAEKKK"
     gene            1172..1717
                     /locus_tag="P5164_19880"
     CDS             1172..1717
                     /locus_tag="P5164_19880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HRY46200.1"
                     /translation="MGDASAAPAEAQGVPWGEVFRLSPGASLLATGLGSGLLPKAPGT
                     WGSLLAVLLAEGLFALYGVGAVALLAALATAFGVPASARVAALRGRKDPSEIVVDEVA
                     GQALALLLLHLALPTGASPILRWGLVAAAFGLFRLLDVVKPGPIDRVQSLPGGWGVMA
                     DDLLAGLGAGILAAAAAFSLR"
     gene            1714..>2043
                     /locus_tag="P5164_19885"
     CDS             1714..>2043
                     /locus_tag="P5164_19885"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRY46201.1"
                     /translation="MIDTSPLTGLRLRLRPEEPLAPRTTWRIGGPARFFAEVEDAPAL
                     AALLRWARAERLPAIPLGKGSNVLVPDEGLDAVVFVLAGDLASVSVEAPVVRAGGGAS
                     LMSLAVTA"
BASE COUNT          223 a          716 c          758 g          346 t
ORIGIN      
        1 ggggagctac ttcttcttct cggccggggc ggcggccgcc gggggagcgg gcggctcgac
       61 gggggagatg ccgagcttct cgcggatctt gttctcgatc tcgagggcga cgtcggggtt
      121 gtcgcggagc cacgccttcg tgttctcgcg cccctggccg atgcgaaggt cgccgtagct
      181 gaaccaggcg ccgctcttct cgacgacctt gtgctcgacg ccgaggtcga tcagctcgcc
      241 ggtcttcgag atcccctcgc cgtagccgat gtcgaactcg gcgaccttga acggcggggc
      301 gaccttgttc ttgacgacct tgaccttcgt ccggctcccg acgacggcgt cgccgtcctt
      361 gatggagttg atccggcgga tgtcgaggcg gaccgaggag tagaacttga gggcccggcc
      421 gcccgtcgtc gtctccgggt tgccgaacat gacgccgatc ttctcccgga tctggttgat
      481 gaagatgagg caggtgcccg acttggagac gacggcggtg agcttgcgga gcgcctgcga
      541 catgaggcgg gcctgcaggc cgaccatcgc gtcgcccatc tctccctcga gctcggcctt
      601 cgggacgagg gccgcgacgg agtcgacgac gacgatgtcg acggcggcgg agcggacgag
      661 cgcctcggcg atctcgagcg cctgctcgcc ggagtcgggc tgggagacca tcaggttgtc
      721 gacgtcgacg ccgagcttgc gggcgtactc ggcgtcgagg gcgtgctcgg cgtcgatgaa
      781 ggcggcgagg ccgcccagcc gctgggcctc ggcgatgatg tgaagcgtga gggtcgtctt
      841 gcccgacgac tcggggccgt agatctcggt gacgcggccg cgcgggacgc ccccgatgcc
      901 gagcgcggcg tcgaggttga tggagccggt cgagatcgcc tggatcgcct cctgcggctt
      961 gtcgccgagg cggacgaggg cgcccttgcc gaactggcgg tcgatctgct ggagcgcctg
     1021 ctccagggcc ttgagctttt ccttctcgat ggggggcatg cgggtcctcg ctttctgcgt
     1081 gaagacggcg gattatagga accgggtggc gggcgcgcgg acagttgatg tcctttcgag
     1141 cgccgcggcc gcccgttcac gaagaggaga cgtgggagac gctagcgcgg cgcccgccga
     1201 ggcgcaaggg gttccgtggg gagaggtctt ccgcctctcg cccggcgcct cgctcctggc
     1261 gacgggtctc ggctcgggcc tcctgccgaa ggcccccggg acgtggggga gcctcctggc
     1321 cgtcctcctc gccgaggggc tcttcgccct ctacggcgtc ggtgctgtgg ccctgctcgc
     1381 ggcgctcgcg acggccttcg gcgtcccggc ctcggcacgc gtcgccgcgc tccggggccg
     1441 aaaggacccg tccgagatcg tcgtcgacga ggtggccggg caggcgctcg ccctcctcct
     1501 cctccacctc gcgctcccca ccggcgcctc gccgatcctc cgctggggcc tcgtggccgc
     1561 ggcgttcggc ctgttccggc tcctcgacgt cgtcaagccc ggcccgatcg accgggtgca
     1621 gtcccttccg gggggctggg gcgtgatggc cgacgacctc ctcgccggac tcggcgccgg
     1681 aatcctggcg gccgccgccg cgttctccct gcggtgatcg acacctcgcc cctcaccggc
     1741 ctccgcctcc ggctccgtcc ggaggagccg ctcgcgccgc ggacgacgtg gcggatcggc
     1801 ggcccggcgc gcttcttcgc cgaggtggag gacgccccgg ctctcgccgc gctcctccgg
     1861 tgggcgcggg cggagcggct cccggcgatc ccgctcggga aggggtcgaa cgtcctcgtc
     1921 cccgacgagg ggctcgacgc cgtagtcttc gtcctcgccg gcgacctcgc gtcggtgtcg
     1981 gtcgaggctc ccgtcgtccg ggccggcggc ggggcctcgc tcatgtcgct cgcggtcacg
     2041 gcg
//
LOCUS       DAOTEZ010000602         2040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_47677_length_2040_cov_6.334005, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000602 DAOTEZ010000000
VERSION     DAOTEZ010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47677_length_2040_cov_6.334005"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="P5164_19890"
     CDS             complement(<1..1118)
                     /locus_tag="P5164_19890"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRY46202.1"
                     /translation="MSRPLLAPLLLVLLARPAAAQAPAGLSDPDALVRERAAAALATT
                     GAAAVDPLAAALADADPYVAGAAADALARIGEASVPALVRALADPREDVRTGAAIALG
                     KLGPEAGGAVPALSKALADPKEVVRWTVANALGAVGRAASPALPALRDALWDRDEDVR
                     RGAALALERIDPAAWLRASSWETTLATIERLVPLLMKEHHVPAVSIALVKDRKVAFSK
                     AWGVADAKTGAPATTATLFEAASMTKPAFAYVALKLVEEGKLDLDRPIAEVVDLPSVP
                     GQAELARITPRRVLSHTSGLPNWRPGGEERDGPLPVLFEPGSRFGYSGEAIYQLQRAV
                     EKVAGAPLEEYAKGALFAPLGMERASFGWAPELDAALAT"
     gene            complement(1115..>2040)
                     /locus_tag="P5164_19895"
     CDS             complement(1115..>2040)
                     /locus_tag="P5164_19895"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRY46203.1"
                     /translation="LLVKRDDLTGLAMGGNKARKLEYDLAPTASGEFDVVLTVGGPQS
                     NHAVLTAAAARRLGLEAKLVLGGPDVAHRKGNLLLGALYGAETRYLLDDDANDSLAAA
                     MEAWACELRATGRRPFLVPLGGSTPNGALGYVRAMRELAAQLGPGKVQLVAAVGSCGT
                     LAGLALGARLFLPAARVVGISVSREADPIAARAAELAAGSAALLGLPPQLGSADVEVH
                     DAFRGEYGVATEAGREAILESARLEGLLLDPVYTGKAMAGFFGLARSGVLDPAVPVVF
                     LHTGGLPILFSFDDDFDDAAPFTKVFPRSAR"
BASE COUNT          263 a          718 c          824 g          235 t
ORIGIN      
        1 gtcgcgagcg cggcgtcgag ctccggggcc cagccaaagg aggcccgctc catgccgaga
       61 ggcgcgaaga gggcgccctt cgcgtactcc tcgagcggcg ctcccgccac cttctcgacg
      121 gcgcgctgga gctggtagat cgcctcgccc gagtagccga agcgggaacc cggctcgaag
      181 aggacgggga gagggccgtc gcgctcctcg cctcccggcc tccagttcgg caggcccgag
      241 gtgtgcgaga ggaccctccg cggcgtgatc cgcgcgagct cggcctggcc cgggacggag
      301 ggaaggtcga cgacctccgc gatcggccgg tcgaggtcga gcttcccttc ctcgacgagc
      361 ttcagcgcga cgtacgcgaa ggccggcttc gtcatcgagg cggcctcgaa gagcgtcgcc
      421 gtcgtggcgg gcgcgcccgt cttcgcgtcg gcgacgcccc aggccttcga gaaggcgacc
      481 ttgcggtcct tcacgagggc gatcgatacg gcagggacgt ggtgctcctt catgaggaga
      541 ggcacgagcc gctcgatcgt cgcgagcgtc gtctcccacg acgacgcgcg aagccacgcc
      601 gcggggtcga tccgctcgag cgcgagagcg gccccgcgcc ggacgtcctc gtcgcggtcc
      661 cagagggcgt cgcggagggc gggaagcgcg ggggaggcgg cgcggccgac ggccccgagg
      721 gcgttcgcca cggtccagcg gacgacctcc ttcgggtcgg cgagcgcctt cgagagcgcg
      781 gggacggccc cgcccgcctc cggcccgagc tttccgaggg cgatcgcggc tccggtccgg
      841 acgtcctcgc gggggtctgc gagcgcgcgg acgagggcgg ggacgctcgc ctcgccgatc
      901 cgtgccaggg cgtcggctgc cgcgccggcg acgtacgggt cggcgtcggc gagggccgcg
      961 gcgagcgggt cgacggccgc cgcgccggtc gtcgcgagag cggccgcggc gcgctcgcgg
     1021 acgagggcgt ccgggtcgga gaggcccgcg ggggcctgcg cggcggccgg gcgggcgagg
     1081 agcacgagga gaagcggggc gaggagaggc cggctcatcg cgcgctccgc gggaagacct
     1141 tcgtgaaggg cgcggcgtcg tcgaaatcgt cgtcgaagga gaagaggatc gggagcccgc
     1201 ccgtgtggag gaagacgacg gggacggccg gatcgaggac gccgcttcgg gccaggccga
     1261 agaagcctgc catcgccttg ccggtgtaga cggggtcgag gaggagcccc tcgagcctcg
     1321 cgctctcgag gatcgcctcg cgccccgctt ccgtcgcgac gccgtactcg ccgcggaagg
     1381 cgtcgtggac ctcgacgtcc gcggagccga gctgcggcgg caggccgagg agcgcggcgc
     1441 ttccggccgc gagctcggcc gcccgggcgg cgatcgggtc ggcctcccgc gaaacgctga
     1501 tcccgacgac gcgcgcggca gggaggaaga gcctcgcgcc gagcgcgagg ccggcgagcg
     1561 tcccgcacga gccgaccgcc gcgacgagct ggaccttccc cgggccgagc tgcgcggcga
     1621 gctcgcgcat cgcccggacg tagccgagcg cgccgttcgg cgtgctcccg ccgagcggga
     1681 cgaggaacgg ccgccgcccc gtggcccgca gctcgcacgc ccaggcctcc atcgccgccg
     1741 cgagggagtc gttcgcgtcg tcgtccagga ggtagcgcgt ctcggcgccg tagagcgcgc
     1801 cgaggaggag gttccctttc cggtgcgcga cgtcggggcc gccgaggacg agcttcgcct
     1861 cgaggccgag gcggcgcgcg gcggcggcgg tcaggacggc gtggttcgac tgcgggccgc
     1921 cgacggtgag gacgacgtcg aactcgccgg aggccgtcgg ggcgaggtcg tactccagct
     1981 ttcgggcctt gttccccccc atcgcgaggc cggtcaggtc gtcgcgcttg acgaggaggc
//
LOCUS       DAOTEZ010000603         2032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_47978_length_2032_cov_6.062721, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000603 DAOTEZ010000000
VERSION     DAOTEZ010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47978_length_2032_cov_6.062721"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="P5164_19900"
     CDS             complement(<1..783)
                     /locus_tag="P5164_19900"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY46204.1"
                     /translation="MGTGFVSCEICRELKTVETSLTKHGWEENDRLLPAAYRRLVEVR
                     VAGREDEPRSPRYCPLCGTFYSYESRYEYFANGSEDEEELRRLTGEEARAWLGDAVVD
                     SFPKPLFSPLSATVVDEHVVVFSTGGEDLASGWGANAVAILGEGETLLVDPLVAPVYA
                     RELARLLAARGAGPVRRVVTTHHHTDHSVGASVFAEAGAEVIAHADSAARMAAEQPAL
                     LAERKSAWPVGTLFFDAVPAAPTRLVTEPLELSVGGVRAVVRP"
     gene            873..>2032
                     /locus_tag="P5164_19905"
     CDS             873..>2032
                     /locus_tag="P5164_19905"
                     /inference="COORDINATES: protein motif:HMM:NF022664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3011 domain-containing protein"
                     /protein_id="HRY46205.1"
                     /translation="MTLSSALARARRGVLAGALLLASPLAGQGPADDYDLVLECRGAL
                     WKATFDDATAAGLDPGAAPVSFHGSEARRTRGDGVVTFRGTEEYQPSHRTRAIPVRYE
                     CVVDTATRKVGSITYEAVDASGAPIAKRPVDLVRDALLLGACRESLEDEVRDEARAGG
                     ASTEGNDLEPDPASVARTEKGKLVELTGTGRARLSRDYEWEPVTFCCRWDPRKEEVTK
                     AWYAPVGARKLGLLTPEKREAIDACRRAIRDAVWDDATARGYRWPRDQVVVELSRRGG
                     FASSGDRVEVKGEGWFKSDARHSQSTPIAFRCAFDANLRAVVDASFEALDASRTPTGE
                     IASGKTGTLVCESFGKAQKVCPASIRGNVRVIRQLGSVPCKAYENWIYSLSG"
BASE COUNT          277 a          770 c          730 g          255 t
ORIGIN      
        1 ggggcggacg accgcccgga cgcccccgac ggagagctcg agcggttccg tcacgagtcg
       61 cgtcggggcg gccgggacgg cgtcgaagaa gagcgtcccc acgggccacg ccgacttcct
      121 ctcggcgagg agcgccggct gctccgcggc catccgcgcg gcggagtccg cgtgggcgat
      181 cacctccgcc cccgcctccg cgaagacgga ggcgccgacg gagtggtcgg tgtggtggtg
      241 ggtcgtcacg acccgccgta ccggcccggc tccccgcgcc gcgaggagac gtgcgagctc
      301 ccgcgcgtag acgggcgcga cgagcgggtc gacgaggagc gtctccccct cgccgaggat
      361 cgccacggcg ttcgcgcccc agcccgaggc gaggtcctcg ccgcccgtcg agaagacgac
      421 gacgtgctcg tccacgaccg ttgcggagag cggggagaag agcggcttcg ggaacgagtc
      481 gacgaccgcg tcgccgagcc aggcgcgcgc ctcctcgccc gtcagccgcc gcagctcctc
      541 ctcgtcctcg ctcccgttcg cgaagtactc gtagcggctc tcgtacgagt agaacgtgcc
      601 gcagagcggg cagtaccggg gcgagcgcgg ctcgtcctcc cgtcccgcga cccgcacctc
      661 gacgaggcgg cggtacgcgg ccgggagcag ccggtcgttc tcctcccagc cgtgcttcgt
      721 gagcgacgtc tcgaccgtct tcagctcgcg gcagatctcg caggagacga acccggtgcc
      781 catcccggga atcgtacgac gcggcgcagc gcgtctcccg ccccgtgcgc ggggcggcgg
      841 cgacggtacg atccgccgca caaggagccg ccatgaccct ctcctccgcg ctcgcgcggg
      901 cgaggcgggg cgtcctcgcg ggcgccctcc tcctcgcctc tccccttgcc gggcagggcc
      961 cggccgacga ctacgacctc gtcctcgagt gccgcggcgc gctctggaag gccaccttcg
     1021 acgacgccac ggccgccggc ctcgacccgg gcgccgcccc ggtctccttc cacggctcgg
     1081 aggcgcggcg aacgcgagga gacggcgtcg tgaccttccg cgggaccgag gagtaccagc
     1141 cgtcgcaccg gaccagggcc atcccggtcc ggtacgagtg cgtcgtcgac acggcgacgc
     1201 ggaaggtcgg ttcgatcacc tacgaggccg tcgacgcctc gggcgcgccg atcgcgaagc
     1261 gccccgtcga cctcgttcgc gacgccctcc tcctcggcgc ctgccgcgag tcgctcgagg
     1321 acgaggtgcg cgacgaggcc cgcgccggcg gcgcgagcac cgaggggaac gacctcgagc
     1381 ccgacccggc ctcggtcgcg cggacggaaa aggggaagct cgtcgagctg accggcaccg
     1441 gccgggcccg gctcagccgg gactacgagt gggagccggt caccttctgc tgccgctggg
     1501 accctcggaa ggaggaggtg acgaaggcct ggtacgcgcc ggtcggcgcc cggaagctcg
     1561 ggctcctgac gcccgagaag agggaggcga ttgacgcctg ccggcgcgcg atccgcgacg
     1621 ccgtctggga cgacgcgacc gcccgcggct atcggtggcc ccgcgaccag gtcgtcgtcg
     1681 agctctcccg tcgcggcggc ttcgcctcct cgggcgaccg cgtcgaggtg aagggcgagg
     1741 ggtggttcaa gtccgacgcc cgccactcgc agtcgacgcc gatcgccttc cgctgcgctt
     1801 tcgacgcgaa cctccgggcg gtcgtcgacg cctccttcga ggcgctcgac gcctcccgca
     1861 cccccacggg cgagatcgcc tcgggaaaga ccggcacgct cgtctgcgag tcgttcggga
     1921 aggcgcagaa ggtctgcccg gcgtcgatcc gcggaaacgt ccgggtgatc cgccagctcg
     1981 gctccgtccc ctgcaaggcc tacgagaact ggatctacag cctctcggga at
//
LOCUS       DAOTEZ010000604         2030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_48056_length_2030_cov_5.237975, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000604 DAOTEZ010000000
VERSION     DAOTEZ010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48056_length_2030_cov_5.237975"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /gene="norR"
                     /locus_tag="P5164_19910"
     CDS             <1..1196
                     /gene="norR"
                     /locus_tag="P5164_19910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HRY46206.1"
                     /translation="CPLTADGEVVGALTADALDPEAFAGLDGPLLATLGALAGAALRT
                     AGLIEALERKAERGTRVARELQRSADETGGAAILGTSASVAALLSEIAVVAASDLPVL
                     VTGETGVGKELVARHVHSGSRRKDEALIHVNCAALPESIAESELFGHVQGAFTGAVRD
                     RAGKFEVADGGSLFLDEVGELPLSLQPKLLRALQQGEIQRIGSDRPHRVDVRVIAATN
                     RDLAAEVARGRFRADLYHRLAVYPIRVPALRERREDIPILATHFADSARRRLGLGPLR
                     LSEEARAALAAAEWPGNVRELENVVSRAVLRAAAGKPPGAPIAVGLENVDLGPGGPVR
                     APVEATAPIRAAGPLREQVEEFQRRRIREAVERHGGNHAAAARELGMHRANLHHLARR
                     LGLRD"
     gene            1280..1882
                     /locus_tag="P5164_19915"
     CDS             1280..1882
                     /locus_tag="P5164_19915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRY46207.1"
                     /translation="MDTSFVSSTILLVLITDPLGNIPFFISALKHVRPERRRVVVLRE
                     CLVAFAALLLFLLAGRPILRLLHLSEDALRISGGVVLLLIAIRMIFPERGARLGEEEG
                     EDREPFIVPVAIPLIAGPSAMATVLLMSTPDPLRMLSLAGSLTVTIAITALVFVSSTR
                     IQKVLGDQAIMAMERLMGLVLTAIAVEMLLGGVASYIRRG"
BASE COUNT          228 a          755 c          774 g          273 t
ORIGIN      
        1 gctgcccgct cacggccgac ggagaggtgg tcggcgcgct gacggccgac gcactcgacc
       61 cggaggcgtt cgctggcctc gacggacccc tcctcgcgac gctcggggcc ctcgcgggcg
      121 cggccctccg gaccgccggc ctcatcgagg cgctcgagag gaaggccgag cgcgggacgc
      181 gcgtcgcgcg ggagctgcag cggagcgcgg acgagacggg cggcgccgcg atcctcggga
      241 cgagcgcctc ggtggcggcg ctcctgtcgg agatcgccgt cgtcgccgcg tcggacctgc
      301 cggtcctcgt gacgggggag acgggcgtcg gcaaggagct cgtcgcgcgg cacgtccact
      361 cgggctcgcg ccggaaggac gaggctctga tccacgtcaa ctgcgcggcg ctccccgagt
      421 cgattgccga gagcgagctc ttcggccacg tgcagggcgc gttcaccgga gcggtccgcg
      481 accgggccgg gaagttcgag gtggccgacg gcggttcgct cttcctcgac gaggtgggcg
      541 agctcccgct ctcgctccag ccgaagctcc tccgggccct ccagcagggg gagatccagc
      601 ggatcggctc ggaccggccg caccgcgtcg acgtgcgggt catcgccgcc acgaaccgcg
      661 acctcgccgc cgaggtggcg cggggccgct tccgcgccga cctctaccac cggctggcgg
      721 tctacccgat ccgggtcccg gcgctccggg agcggcggga agacatcccg atcctcgcga
      781 cccatttcgc cgactccgcg cgccgccgtc tcgggctcgg cccgctccgc ctctcggagg
      841 aggcccgggc ggcgctcgcc gccgcggagt ggcccgggaa cgtccgggag ctcgagaacg
      901 tcgtcagccg ggccgtgctg cgggccgcgg ccgggaagcc gccgggcgcg ccgatcgcgg
      961 tcggactcga gaacgtcgac ctcgggcccg ggggcccggt tcgggcgccg gtcgaggcga
     1021 cggcgccgat ccgtgcggcc ggccctcttc gcgagcaggt cgaggagttc cagcggaggc
     1081 gcatccgcga ggcggtggag cggcacggag ggaaccacgc cgccgccgcg cgcgagctcg
     1141 ggatgcaccg cgcgaacctc caccacctcg cacggcggct cggcctgcgg gactgacggc
     1201 gccgggaccc ggggccgcgc ccggcgggcg cggagagccg gcgggagggc ggccgttccc
     1261 gatatcctcg gcgggcccca tggacacgag cttcgtctcc tccaccatcc tcctcgtcct
     1321 gatcaccgac ccgctcggga acatcccctt cttcatctcc gcgctgaagc acgtccgccc
     1381 cgagcgccgc cgcgtcgtcg tcctccgcga gtgcctcgtc gcgttcgccg cgctcctcct
     1441 cttcctcctc gcgggtcggc cgatcctccg gctgctccac ctgagcgaag acgccctccg
     1501 catcagcggc ggtgtcgtcc tgctcctgat tgcgatccgg atgatcttcc cggagagggg
     1561 ggcgcgcctc ggcgaggagg agggagagga ccgcgagcct ttcatcgtgc cggtggcgat
     1621 cccgttgatc gcggggccct cggcgatggc gacggtcctc ctgatgtcga cgcccgatcc
     1681 gctccggatg ctctcgctcg cgggctcgct gacggtgacg atcgcgatca cggcgctcgt
     1741 cttcgtctcg agcacgcgga tccagaaggt gctcggggac caggcgatca tggcgatgga
     1801 gcgcctgatg gggctcgtcc tgacggcgat cgccgtcgag atgctcctgg gcggcgtcgc
     1861 gagctacatc cgccgcggct gaggggtcgg ggccgcttcc caggggggcg cggccgtcgt
     1921 cggggcctcc ggcgtccgcg gcagtggcct cggcctgcgc gcgggagggg gcgcgggcgt
     1981 ccccgcgggc gtcggcgccg gggtcggtct cgcagagccg gcgcgcgggg
//
LOCUS       DAOTEZ010000605         2026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_48216_length_2026_cov_5.429731, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000605 DAOTEZ010000000
VERSION     DAOTEZ010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48216_length_2026_cov_5.429731"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="P5164_19920"
     CDS             <1..523
                     /locus_tag="P5164_19920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46208.1"
                     /translation="ERLFRLGFPVLGKAPVSFPFFSEQRRIDLVLFSVVLPPPLASAS
                     STSGPLPWKVGAPLLGSLDGPVEGQTVTGDLLVRGWAREPGEDLRVSILIDGAEVPPR
                     EFRRVPRPDVPRVFPDLGDCRWAGYEAIVERPAGTKPEPVVSVVFRSRDGRSRHYPGV
                     RIRWGDAASVAPR"
     gene            complement(554..>2026)
                     /locus_tag="P5164_19925"
     CDS             complement(554..>2026)
                     /locus_tag="P5164_19925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HRY46209.1"
                     /translation="RDEMRENLLAMLARKESPFPGILGYERTVVPALVNAVLARHDVV
                     LLGLRGQAKTRILRSLVNLLDEETPVIVGSELNESPFAPFTRYARNLAATAGPDLPVG
                     WLPRRARYREKLATPDVTIADLIGDVDPVKAATLKKTFADEEVLHYGIVPRTNRGIFA
                     INELPDLAPRIQVGLLNILEERDLQIRGFPVRIPLDVVMVFSANPEDYTNRGSIITPL
                     KDRIASQVLTHYPRDLATAKAITDQEAWCDRPVEGVEVRLPEPFKVLVEEVAFAARRS
                     DLVDQASGVSARMPIAMRELLVSNLERRALLTGETVVSPRLVDLAATLPAITGKVEMV
                     FEGEQQGAEIVARKLVGEAVKTLFESTFPPIEGPRARGERARRREADDGTAVPFVRER
                     APLSGPYERVVTWFTKERTLVLSDETPFARHLASLDAVDGLAELVAAHAKPSDEQERA
                     FLMELVLEGLVQSLRIAREDLDSTVTYVELARLNLMRGRD"
BASE COUNT          262 a          701 c          749 g          314 t
ORIGIN      
        1 ggagcggctc ttccggctcg ggttccccgt cctcgggaag gcccccgtct cgttcccgtt
       61 cttctcggag caacgccgca tcgacctcgt tctcttctcg gtcgtcctcc ctccgccgct
      121 cgcgtcggcg agctcgacgt ccgggccgtt gccctggaag gtcggggcgc cgctcctcgg
      181 gagcctggac ggtcccgtcg aagggcagac cgtgacgggc gacctcctcg tgagaggctg
      241 ggcccgcgag cccggagagg acctgcgcgt ctcgatcctg atcgacgggg ccgaggtgcc
      301 gcctcgcgag ttccgccgcg tgccgcgacc ggacgtccct cgggtcttcc ccgacctggg
      361 cgactgccgg tgggccggct acgaggcgat cgtcgaacgt ccggccggga cgaagcccga
      421 gccggtcgtg tcggtcgtct tccgctcccg cgacggccgg agccggcact atccgggcgt
      481 ccggatccgc tggggcgacg cggcgtccgt cgcgccccgc tgacctcggc cttcgaaacg
      541 ggaagcgccg ccctcagtcc cggccgcgca tcaggttcag tcgcgccagc tccacgtagg
      601 tgacggtcga atcgaggtct tcgcgcgcga tgcggaggct ctggacgagc ccttcgagga
      661 cgagctccat caggaacgcg cgctcctgct cgtcggacgg cttcgcgtgc gcggcgacga
      721 gctcggcgag cccgtcgacg gcgtcgagcg aggcgaggtg cctcgcgaac ggcgtctcgt
      781 cggagaggac gagcgtccgc tccttcgtga accaggtgac gaccctctcg tagggcccgg
      841 agagcggagc acgctcgcgg acgaacggca cggccgtgcc gtcgtccgcc tcgcggcggc
      901 gcgccctctc gccgcgcgcg cgcggtccct cgatcggcgg gaaggtcgac tcgaagagcg
      961 tcttcaccgc ctcgccgacg agctttcggg ccacgatctc ggccccctgc tgctcgccct
     1021 cgaagaccat ctcgaccttg cccgtgatcg ccggaagcgt cgcggcgagg tcgacgaggc
     1081 ggggggagac gaccgtctct cccgtgagga gggcgcgccg ctcgaggttc gagacgagga
     1141 gctccctcat cgcgatcggc atccgggccg agacgcccga ggcctggtcg acgaggtcgc
     1201 tccggcgggc ggcgaaggcg acctcctcga cgaggacctt gaagggctcg gggagccgga
     1261 cctcgacgcc ctcgacgggg cggtcgcacc aggcctcctg gtcggtgatc gccttcgccg
     1321 tcgcgaggtc gcgcgggtag tgcgtcagga cctgagaggc gatccggtcc ttcaggggcg
     1381 tgatgatgct gccgcggttc gtgtagtcct cggggttcgc ggaaaagacc atcacgacgt
     1441 cgagcgggat ccgcacgggg aagccgcgga tctggaggtc gcgctcctcg aggatgttga
     1501 ggagcccgac ctggatccgc ggcgcgaggt ccgggagctc gttgatcgcg aagatgccgc
     1561 ggttcgtccg aggcacgatg ccgtagtgga ggacctcctc gtcggcgaac gtcttcttca
     1621 gcgtcgccgc cttcaccgga tccacgtcgc cgatcaggtc ggcgatcgtc acgtcgggcg
     1681 tcgcgagctt ctcgcggtag cgcgccctgc gggggagcca gccgaccggg aggtccggcc
     1741 cggccgtggc ggcgaggttt cgggcgtatc gcgtgaacgg cgcgaacggc gactcgttca
     1801 gctcggagcc gacgatcacc ggcgtctcct cgtcgaggag gttcacgagg gagcggagga
     1861 tgcgcgtctt ggcctggccg cggaggccga ggaggacgac gtcgtggcgg gcgaggacgg
     1921 cgttcacgag ggccggcacg accgttcgct cgtagccgag gatgcccggg aacgggctct
     1981 ccttccgcgc cagcatcgcg aggaggttct cgcgcatctc gtctcg
//
LOCUS       DAOTEZ010000606         2024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_48306_length_2024_cov_5.871508, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000606 DAOTEZ010000000
VERSION     DAOTEZ010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48306_length_2024_cov_5.871508"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="P5164_19930"
     CDS             <1..1011
                     /locus_tag="P5164_19930"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HRY46210.1"
                     /translation="PVEVNVTYNSPKVTAPDGTDRRGKIWGGLVPWGMSKDSFGTCGE
                     ECPWRAGANENTVFRTSHDVRIEGQPLAAGSYGLFMIPGPEEFTVIFSRNSTSWGHYF
                     YDAKEDALRVKVKTRKAPYTHWMTFDFVERKPAEATVALRWDELEVPVRVEVPDVDGL
                     YLGEIRNQLRNATGFDWQAWEQAAQFCLSRKINLPEALKWAEHAATPGFTGQESFRTL
                     STLSQAQAANGMADAAQATMQKAMAHPTATAIDLHMYARGLQAQKKTDEAVAVYLLNA
                     KKHPGQWPVEVGLARAYSAQGKLKDALSAAKKALAQAPDDPNRKGLQGMIEKLSAGKP
                     ID"
     gene            complement(1076..>2024)
                     /locus_tag="P5164_19935"
     CDS             complement(1076..>2024)
                     /locus_tag="P5164_19935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46211.1"
                     /translation="VRVTRLEGSAPWIAYGVVNDGGAPGERTGDGAFVPAVLPATPVT
                     DGALVVDHTCTDLSRVPARYLEAAKAQLKIGYGHTSHGSQVVTGMEALAAAGALLDFD
                     SSWGYLPGVFLNDSAFPGANDLGSPDRTAWADATRELLSRAGGCDRNVVVWSWCGQAD
                     TGDPRDIETYLSLMSALEEEFPAVRFVYMTGHLVGSGAGGDLHRRNEQIRRYCRENGK
                     VLFDFADIESFDPDGLVDFMKKYATDGCEYDRNGDGNPWGDGNWAREWISAHPGHELA
                     QLAASCGDCAHSERLNCVLKARAFWWMLARLAGWDGTSG"
BASE COUNT          316 a          709 c          732 g          267 t
ORIGIN      
        1 ccggtcgagg tgaacgtcac ctacaacagc ccgaaggtga cggctcccga cggaaccgac
       61 cggcgcggga agatctgggg cggcctcgtc ccctggggga tgtcgaagga ctccttcggc
      121 acctgcggcg aggagtgccc gtggcgggcc ggcgcgaacg agaacaccgt cttccggacc
      181 tcgcacgacg tgaggatcga ggggcagccg ctcgcggcgg gctcgtacgg ccttttcatg
      241 atcccggggc cggaggagtt cacggtcatc ttctcgagga actccacgag ctggggacac
      301 tacttctacg acgcgaagga ggacgcgctc cgcgtgaagg tgaagacgcg gaaggcgccg
      361 tacacgcact ggatgacgtt cgacttcgtc gagcgcaagc ccgccgaggc gaccgtcgct
      421 ctccgctggg acgagctgga ggtcccggtc cgggtcgagg tccccgacgt cgacggcctc
      481 tacctcggcg agatccggaa ccagctccgc aacgcgacgg ggttcgactg gcaggcgtgg
      541 gagcaggccg cgcagttctg cctctcccgg aagatcaacc tccccgaggc gctgaagtgg
      601 gccgagcacg ccgcgacgcc gggcttcacc gggcaggaga gcttccggac cctctcgacc
      661 ctctcgcagg cccaggcggc gaacgggatg gccgacgccg cgcaggcgac gatgcagaag
      721 gcgatggccc acccgacggc gacggcgatc gacctgcaca tgtacgcgcg cggtctccag
      781 gcgcagaaga agaccgacga ggccgtcgcc gtctacctcc tgaacgcgaa gaagcacccg
      841 ggccagtggc cggtcgaggt cggcctggcg cgggcgtact cggcgcaggg gaagctgaag
      901 gacgccctct ccgccgcgaa gaaggcgctc gcgcaggcgc ccgatgaccc gaacaggaag
      961 ggcctgcagg ggatgatcga gaagctctcg gcgggcaagc cgatcgactg aggacgaaag
     1021 ggcgccgcgc gggggccgac cccgcgcggc gcccgggctc ccggcgtccc ctccgctaac
     1081 cgctcgtccc gtcccacccg gcgaggcgcg cgagcatcca ccagaacgcg cgcgccttca
     1141 ggacgcagtt gaggcgctcg gagtgggcgc agtcgccgca ggaggcggcg agctgcgcga
     1201 gctcgtgccc cgggtgggcg gagatccact cccgggccca gttcccgtcg ccccacgggt
     1261 tcccgtcgcc gttccggtcg tactcgcatc cgtccgtcgc gtacttcttc atgaagtcga
     1321 cgagcccgtc ggggtcgaag ctctcgatgt cggcgaagtc gaagaggacc ttgccgttct
     1381 cccggcagta ccgccggatc tgctcgttcc gccggtggag gtccccaccg gccccgctcc
     1441 cgacgagatg gcccgtcatg tagacgaagc ggaccgcggg gaactcctcc tcgagcgcgc
     1501 tcatcagcga caggtacgtc tcgatgtctc gcgggtcgcc cgtgtcggcc tgcccgcacc
     1561 acgaccagac gacgacgttc cggtcgcagc ctccggcgcg gctcaggagc tcgcgggtcg
     1621 cgtcggccca ggcggtccgg tcggggctcc cgaggtcgtt cgccccgggg aaggccgagt
     1681 cgttcaggaa gacgccgggg aggtatcccc aggaagagtc gaagtccagg agcgccccgg
     1741 ccgcagccag cgcttccatc ccggtcacga cctggctgcc gtgagaggtg tggccgtagc
     1801 cgatcttcag ctgcgccttc gcggcctcga ggtatcgcgc cggcacgcgg gagaggtcgg
     1861 tgcaggtgtg gtcgacgacg agggcgccgt ccgtgacggg cgtcgccggg aggaccgcgg
     1921 ggacgaaggc gccgtcgccg gtccgctccc cgggcgcgcc gccgtcgttc acgacgccgt
     1981 aggcgatcca cggcgcgctc ccctcgaggc gcgtcacgcg gacg
//
LOCUS       DAOTEZ010000607         1983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_49986_length_1983_cov_7.999481, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000607 DAOTEZ010000000
VERSION     DAOTEZ010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49986_length_1983_cov_7.999481"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="P5164_19940"
     CDS             complement(<1..254)
                     /locus_tag="P5164_19940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY46212.1"
                     /translation="MSIEVRSVSKSFGSFLALDEVSLRVGDGELVALLGPSGGGKTSL
                     LRIIAGLEEPDRGSVHFHGEDATRRDARERRVGFVFQHYAL"
     gene            complement(251..1078)
                     /gene="cysW"
                     /locus_tag="P5164_19945"
     CDS             complement(251..1078)
                     /gene="cysW"
                     /locus_tag="P5164_19945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HRY46213.1"
                     /translation="MSASRSREPRAVRLLLIGLALAAFVLFLLVPLVAVFVEAFRDGA
                     RAWLAAVTEPDARLALKLTLLVAAIAVPVNAFFGLAAGWALGKFRFPGRSLVLTLVDL
                     PFAVSPVVAGLVFVLLFGRTGFFGPWLAGRGVEVVFALPGIVLATVFVTFPFVAREVV
                     PLMQSQGTDEEEAARVLGAGFFTTFFRVTLPNVKWALLYGIVLCTARAVGEFGAVSVV
                     SGHVRGETNTLPLHVEALYNEYAFQGAFAAASLLTFVALATMVAKAIVSRRAREEAP"
     gene            complement(1075..1905)
                     /gene="cysT"
                     /locus_tag="P5164_19950"
     CDS             complement(1075..1905)
                     /gene="cysT"
                     /locus_tag="P5164_19950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HRY46214.1"
                     /translation="MSRRRVSPSLLPGFGPALGVTLLLLSLVVLAPLAALLATTLRGT
                     PREFLAAVSTPRVVAAMRLSFLVSLGAAAVSALLGLLLGWVLVRYRFPGRRLLDALVD
                     LPFALPTAVAGITLTTLYAPTGWLGRPLAALGVPVAFTPLGIGVALVFLGLPFTVRTL
                     QPVLASLDPEVEEAAASLGATRLDTFRRVLLPELLPSLLTGTTLAFARGLGEYGSVVF
                     ISGNMPMKTEIAPLLVMTKLEQYDVAGATALATLLLVASFLLLLVLNRLEAWAARAVR
                     "
BASE COUNT          357 a          626 c          814 g          186 t
ORIGIN      
        1 agggcgtagt gctggaagac gaagccgacg cgccgctcgc gggcgtcgcg gcgcgtggcg
       61 tcctcgccgt ggaaatggac ggacccgcgg tcgggctcct ccaggccggc gatgatccgg
      121 agaaggctcg tctttccgcc tcccgagggg ccgaggaggg cgacgagctc gccgtcgccg
      181 acgcggaggg agacctcgtc gagcgcgagg aagctcccga acgacttcga gacggaccgg
      241 acctcgatgc tcaaggggcc tcctccctcg cgcggcgcga gacgattgcc ttcgcgacca
      301 tcgtggcgag ggcgacgaag gtcaggagcg aggcggccgc gaacgccccc tggaaggcgt
      361 actcgttgta gagcgcctcg acgtggaggg ggagcgtgtt cgtctccccg cggacgtgcc
      421 ccgagacgac ggagaccgct ccgaactcgc cgacggcgcg ggcggtgcag aggacgatgc
      481 cgtagaggag cgcccacttc acgttcggga gcgtcactcg gaagaacgtc gtgaagaagc
      541 cggcgccgag gacccgggcc gcctcttcct cgtcggtccc ctgcgactgc atgagcggga
      601 cgacctcgcg ggcgacgaac gggaacgtca cgaagacggt cgccaggacg atgcccggga
      661 gggcgaagac gacctcgacc ccgcggccgg cgagccacgg cccgaagaag ccggtgcgcc
      721 cgaagaggag gacgaagacg aggccggcga cgacggggga gaccgcgaac ggcaggtcga
      781 cgagcgtcag gacgaggctg cgccccggga agcggaactt cccgagcgcc cagccggcgg
      841 cgaggccgaa gaacgcgttc acggggacgg cgatcgccgc gacgaggagc gtcagcttca
      901 gcgcgagccg ggcgtcgggc tcggtgacgg cggcgagcca cgcccgcgcg ccgtcccgga
      961 aggcctcgac gaagacggcc acgagcggga cgaggaggaa gaggacgaat gcggcgaggg
     1021 cgaggccgat caggaggagc cgcacggcgc gcggctcgcg gctccgggaa gcgctcaccg
     1081 gacggccctc gccgcccacg cttcgaggcg gttcaggacg aggaggagga ggaaggaggc
     1141 gacgaggagg agcgtcgcga gcgcggtcgc cccggccacg tcgtactgct cgagcttcgt
     1201 catgacgagg agcggagcga tctcggtctt catgggcatg ttcccggaga tgaagacgac
     1261 cgacccgtat tcgccgaggc cccgcgcgaa tgcaagcgtg gtcccggtga ggagcgaagg
     1321 gaggagctcc gggaggagga cacggcggaa agtgtcgagc cgcgtcgcgc cgaggctcgc
     1381 cgcagcctcc tcgacctccg ggtcgaggct cgcgaggacc ggctggagcg tcctcacggt
     1441 gaatgggagg ccgaggaaga cgagcgcgac gccgatgccg agcggggtga acgcgaccgg
     1501 gacgccgagc gcggcgaggg gccggccgag ccagccggtc ggggcgtaga gcgtcgtgag
     1561 cgtgatcccg gcgacggcgg tcgggagcgc gaacgggagg tcgacgaggg cgtcgaggag
     1621 gcgccgtccc gggaagcggt agcggacgag gacccagccg aggaggaggc cgaggagcgc
     1681 ggagaccgcc gccgccccga gcgagacgag gaacgagagc ctcatcgcgg cgacgacgcg
     1741 cggcgtcgag acggccgcga ggaactcgcg cggggtgccg cgaagggtcg tagccaggag
     1801 cgcggcgagc ggcgcgagga cgacgagcga gaggaggagg agcgtcaccc cgagcgccgg
     1861 gccgaagccc gggaggaggg acggggagac gcgccgtcgg ctcacttcgt ggtgaacagc
     1921 gagtcgaaga cgccgccgtc gtcgaagtgc ctcttctgcg cggcgcgcca gccgccgaag
     1981 agc
//
LOCUS       DAOTEZ010000608         1981 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_50078_length_1981_cov_4.397715, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000608 DAOTEZ010000000
VERSION     DAOTEZ010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50078_length_1981_cov_4.397715"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1899)
                     /locus_tag="P5164_19955"
     CDS             complement(<1..1899)
                     /locus_tag="P5164_19955"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRY46215.1"
                     /translation="MRKTLLPFLLAALLAPACRGGKTDGPRAFPGAPVVLVCVDTLRS
                     DRLPFYGYGKVETPALTALREEAVLFERAYSHVPLTLPAHASIFTGALPGAHGVLDNA
                     GYRLPDTLPTLAELLSKEGYVTGAAVSSVVLAGSSGISRGCTLWNDAIVPERAGVPVN
                     RVQRPGAETAEILARFVEERKDSPFFAFLHVYEPHAPYEAPEPFRSRFADPYDAEVAA
                     ADAAVGALVAKLKALGLWEKSLVVFLSDHGEGLGEHGENEHGVFLYRESLQVPLLLKL
                     PKGTRGGTSVAAPVQISDVFRTIVEAAGLSGAPVPADLVNLVGLAYGDAAPDRRIYAE
                     TFFPRIRFGWSELAALLDGSWHYVAAPKPEFYDLEADPGSTRNLAEEKPGPFRSFVVE
                     TEKLRSPFRAPADVDPEHAKKLASLGYLSMTTAGGSEARPDPKDEIGSLEKLKEGLGH
                     LQADRPAEAVAALQQLLERNPRVLDAWELLAQALARLGRPDEALAAIRKTVELSPPDR
                     TSALLTVAAFCLQIGRPDEALAQARVARDLGDRNADEVIARAELARGNADAAETAARH
                     ALEGSPSSRPGALFALARAAALRGDVNGALARTEEVRAALGGRAEVASLQGFHALRGD
                     LLARLGRPA"
BASE COUNT          265 a          697 c          774 g          245 t
ORIGIN      
        1 ggcggggcgc ccgagccgcg cgaggagatc gccgcggagg gcgtggaagc cctggagcga
       61 ggcgacctcg gcccgccctc cgagggcggc gcggacctcc tcggtacgcg cgagggcgcc
      121 gttcacgtcg ccccggagcg ccgccgcgcg ggcaagcgcg aagagcgcgc cgggccggct
      181 cgagggactc ccttccaggg cgtgccgcgc ggccgtctcg gcagcatccg cgttcccgcg
      241 ggcgagctcg gcccgggcga tcacctcgtc ggcgttccgg tccccgaggt cccgcgcgac
      301 gcgcgcctgg gcgagcgcct cgtcgggccg gccgatctgg aggcagaaag ccgccaccgt
      361 caggagcgcg ctcgtccggt cgggcggcga gagctccacc gtcttccgga tcgccgcgag
      421 cgcctcgtcg gggcggccga ggcgcgcgag cgcctgggcg agcagctccc aggcgtcgag
      481 gacgcgcggg ttccgctcga ggagctgctg gagcgcggcg accgcctccg cggggcggtc
      541 cgcctggagg tggccgagcc cctccttcag cttctcgagg ctcccgatct cgtccttcgg
      601 gtcggggcgc gcctccgacc cgcccgccgt cgtcatgctc aggtagccga gcgaagcgag
      661 cttcttcgcg tgctccgggt cgacgtcggc cggggcgcgg aacggcgagc ggagcttctc
      721 cgtctcgacg acgaacgatc ggaacggccc cggcttctcc tcggcgaggt tgcgcgtcga
      781 gcccgggtcg gcctcgaggt cgtagaactc gggcttcggc gcggcgacgt agtgccacga
      841 gccgtcgagg agcgccgcga gctcgctcca gccgaagcgg atccggggga agaacgtctc
      901 ggcgtagatc cggcgatcgg gcgccgcgtc tccgtacgcg aggccgacga ggttcacgag
      961 gtccgcgggg accggtgccc ccgagaggcc ggccgcctcg acgatcgtcc ggaagacgtc
     1021 gctgatctgg acgggggcgg cgacgctcgt cccgccgcgg gttcccttcg ggagcttcag
     1081 caggagcggc acctggagcg actcgcggta gaggaagacg ccgtgctcgt tctccccgtg
     1141 ctcgccgagc ccctcgccat ggtccgagag gaagacgacg aggctcttct cccagaggcc
     1201 gagcgccttc agcttcgcga cgagtgcccc gaccgcggcg tccgcggcgg cgacctcggc
     1261 gtcgtacggg tcggcgaagc ggctccggaa cggctcgggc gcctcgtacg gcgcgtgcgg
     1321 ctcgtagacg tggaggaacg cgaagaacgg gctgtccttc ctctcctcga cgaagcgcgc
     1381 gaggatctct gcggtctccg ctccgggcct ctggacgcgg ttcaccggga cgcctgccct
     1441 ctccggcacg atcgcgtcgt tccagagcgt gcagccgcgg gaaatgccgc tcgagccggc
     1501 gaggacgacg ctcgaaacgg ccgcccccgt cacatacccc tccttcgaga ggagctcggc
     1561 gagcgtcggg agggtgtccg ggaggcgata gccggcgttg tcgaggacgc cgtgcgcccc
     1621 cgggagcgcc ccggtgaaga tcgaggcatg ggccgggagc gtcagcggga cgtggctgta
     1681 ggcccgctcg aagaggaccg cctcctcgcg cagcgccgtc agggccggcg tctcgacctt
     1741 cccgtagccg tagaagggga gccggtcgga gcggagcgtg tcgacgcaga cgaggacgac
     1801 cggcgcgccg gggaaggccc gcgggccgtc ggtctttccc ccacggcacg cgggagcgag
     1861 gagcgcggcg aggaggaagg ggaggagcgt cttccgcacg gcgccggcga gattagcagg
     1921 gcgggccgcg agagcgcgga gacgaaaaag ggccccggcc cgaaggccgg ggcccgtgaa
     1981 a
//
LOCUS       DAOTEZ010000609         1971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_50525_length_1971_cov_4.114301, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000609 DAOTEZ010000000
VERSION     DAOTEZ010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50525_length_1971_cov_4.114301"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..1961
                     /locus_tag="P5164_19960"
     CDS             57..1961
                     /locus_tag="P5164_19960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY46216.1"
                     /translation="MAIKILLADDSLTIQKVVELTFSDAETRLMAVGSGDKAVQALDE
                     FQPDIVLADVVMPGLTGYDVCEAVKQRPGGAFVPVVLLTGTFEPFDRARADRVGSDAI
                     VTKPFDSHALQGLVRELVAKANAAREAAATAPPPAPEPPPPPVFTLSDLEASEEPAAP
                     VDTGSTLVLTPLDLDAAAPPPFAEAAAAAPVGESIYATMAMKLPTPEELAAAAPPPFE
                     LAEELPAAEGPAPFEPEEPLLEPVAAAETFEPAPPFEVEETPEVTPSADVAAPPEEEA
                     PWSEEPAQETPPPAEPVLSIEPPPPSYDPLAAYAPPPPPPFVPAEAFVPPPPPPAFYE
                     PEPPAPPPPPRFFEPEPVVATELDTAPVPPLPPTEEPEPAPFEAEPAVKAAAEAPMPI
                     ELDAEGEPAPAEPQFEPTVADEVGREEAAAEVPSEVVEPFPEMEADARGAEEAPWDEG
                     PALQVPPPPDLGAGPEELSDGTEPVTRDLEADMQAFEESGRVRRRPEIWERHAALIGE
                     EVPEDIDVPGVVPEAPVATPEEPAASDLEEIAAAARLEDLSALIPAGAASAPGELAVV
                     PEPVAAVTTAPIAPAPAAGLSDADVDRIARRLVELLSDRIVRDIAWEVVPEYAERLVR
                     ERIAEVERAG"
BASE COUNT          261 a          775 c          692 g          243 t
ORIGIN      
        1 cgtagacttt ccgccacgta agcccgtcgc gtcgaacggc cgttcgagga gtcgagatgg
       61 cgatcaagat cctgctcgcg gacgacagcc tgacgatcca gaaggtcgtc gagctgactt
      121 tctcggatgc cgagacccgc ctgatggcgg tcgggagcgg cgacaaggcc gtccaggccc
      181 tcgacgagtt ccagcccgac atcgtcctgg ccgacgtcgt catgcccgga ctgacgggct
      241 acgacgtctg cgaggcggtg aagcagcggc cgggcggcgc cttcgtcccg gtggtcctcc
      301 tcaccggcac gttcgagccg ttcgaccggg cccgcgcgga tcgcgtcggc agcgacgcca
      361 tagtcacgaa gccgttcgac tcgcatgccc tgcaggggct cgtgcgggag ctcgtcgcga
      421 aggcgaacgc cgcccgggag gcagcggcga cggcgccgcc gccggctcct gagccgcccc
      481 ctccgccggt cttcacgctc tccgacctgg aggcgtcgga ggagcccgcg gcgccggtcg
      541 acaccggctc gacgctcgtc ctcacgcccc tcgacctcga cgccgccgcg ccgccgcctt
      601 tcgccgaggc tgccgcggcg gcgcccgtcg gagagtcgat ctacgccacg atggcgatga
      661 agctgccgac ccctgaggag ctcgccgcgg cggccccccc gcccttcgag ctggcggagg
      721 agctgcccgc tgccgaaggt ccggcgccct tcgagcccga ggagccgctc ctcgagccgg
      781 tcgccgctgc cgagaccttc gagcctgcgc cgccgttcga ggtcgaagag acgcccgagg
      841 tcacgccgtc ggccgacgtc gcagcgcccc cggaagaaga ggccccctgg agcgaggagc
      901 cggcacagga gacgccgccc ccggccgagc ccgtcctttc gatcgagcct cctcctccct
      961 cctacgaccc gctcgcggcg tacgcccctc cgcccccgcc tcctttcgtg ccggccgagg
     1021 cgttcgtgcc gccgcccccg ccccccgcgt tctacgagcc ggagccgccg gccccgccgc
     1081 ctccgccgcg gttcttcgag cccgagccgg tcgtcgcgac cgagctcgac acggctcccg
     1141 taccgcctct cccgccgact gaagagccgg agcccgcgcc gttcgaggcc gagcccgcag
     1201 tcaaagccgc cgccgaggcg ccgatgccga tcgagctcga tgcggaaggg gagcccgccc
     1261 cggccgagcc gcagttcgag ccgacggtcg ccgacgaggt cggccgggag gaggcggccg
     1321 ccgaagtccc gtccgaggtg gtcgagccgt ttcccgagat ggaggcggat gctcgcggcg
     1381 cggaggaagc tccgtgggac gaggggcccg cgctccaggt gccgccgccg cccgacctcg
     1441 gcgccgggcc cgaggagctt tccgacggaa ccgagccggt cacccgagac ctcgaagccg
     1501 acatgcaggc cttcgaggag tccggccgcg tccgccgcag gcccgagatc tgggaacgtc
     1561 atgccgcgct gatcggcgag gaggtgcccg aggacatcga cgtcccgggc gtcgtccccg
     1621 aggcgcccgt cgcgacgccg gaagagccgg ccgcgagcga cctcgaagag atcgccgcgg
     1681 cggcgcgcct cgaggacctc tccgccctga tcccggccgg agcggcctcc gcgcccggag
     1741 agctggccgt cgtccccgag cccgttgcag ccgtcacgac ggcgccgatc gctccggcgc
     1801 cggccgcggg cctttccgac gccgacgtcg accggatcgc ccgccggctg gtcgagctcc
     1861 tctccgacag gatcgtccgc gacatcgcct gggaggtcgt tcccgagtac gccgaacggc
     1921 tcgtccgcga gaggatcgcc gaggtcgagc gggcgggctg agccggccgc g
//
LOCUS       DAOTEZ010000610         1937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_51992_length_1937_cov_6.562168, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000610 DAOTEZ010000000
VERSION     DAOTEZ010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51992_length_1937_cov_6.562168"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            759..1385
                     /locus_tag="P5164_19965"
     CDS             759..1385
                     /locus_tag="P5164_19965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46217.1"
                     /translation="MSPIVGVFLLLIPAFPAAAQAGAVEGTWLLTQGVDLGLAAGEGP
                     GLQLPIAFELLHLDEDTEVREQSSLFRAETCGREIPVGNGLRMTLPFSAEPGIGHWSY
                     SGNVVRVSSYHMLYDCSGDPLGVAWAVREATVVHRAVAPGPEEASDQTALRVVFWQGE
                     ATIRFFTLTGEPLPLPLFFRPPFPGTDSGVSELSGSFRAVRAFGEPSR"
     gene            complement(1473..>1937)
                     /locus_tag="P5164_19970"
     CDS             complement(1473..>1937)
                     /locus_tag="P5164_19970"
                     /inference="COORDINATES: protein motif:HMM:NF036996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase GatDE subunit D"
                     /protein_id="HRY46218.1"
                     /translation="VYDDRTTILYYYPGMNPDLVDALVEKGYHGIVIAGTGLGHVNKP
                     LYPAIKRATEAGVHVVMAVQTIWGFAQMYVYDTGRDLMDLGIVPLDNMIPETALMKLS
                     WVLGHTRERAEVMRMMTTPVAHEITPREPHNGYLVMQGGLPETEEWVRGHWK"
BASE COUNT          258 a          690 c          694 g          295 t
ORIGIN      
        1 aggaagagga acgggagctt cccctcgacg accttccagt cggccgggtt gagcgtcgcc
       61 gccacgacgc ggacgacgac ctgcccgggg cccggccgcg ggtcgaagac cttcttcacg
      121 accccctcgc gcgggtcgcc gtgcttctcg taggtgagag cgcgcatcgg acctcccgga
      181 atcccgctcc ccggacgcgg gacgccggcc atcgtaccgg ttcccgcacg cggccggcgg
      241 gcgggaggcg gccgggagta cgcccgtgac gtggcaccgg aagacgcgca gactggcgac
      301 gggcgccgcg ccgggcacga aggcgcggcc gcgagggagc tcgagactcc tcgcgtacgc
      361 ctcgtccctc agccggcctc gccgtgaacg agcgctaccg tcgaacgctc gagctcctct
      421 cccgccacgg cgtcgtcatc atccgcatca tcccggaggc cggagacgcg cgggtcggac
      481 cgctcggggg tcctgcccgg acacggcaga cgggagcccg gcggcctcct tgtcgctgcc
      541 gtgcgccccg aggttggacg ggaggaaggc ctcggccccg acggcaggag cggcgaggtg
      601 gtggaacctc gtgggctacg ccaccgggcg ccggtccaag gaagaaccca gcgaattccc
      661 ggtggcggct cccccgcagc gggcctacgt atcgggtgag gcgtcctctg gacacttccc
      721 ggaacgcctc aggaggcaag gccatgcgaa gtctgcccgt gtcgccgatc gtaggagtct
      781 tccttctcct gattccggcg ttccccgctg ccgcccaggc gggggcggtc gaagggacct
      841 ggctcctgac ccaaggggtc gacctggggc tcgcggccgg ggagggcccg ggtctgcagc
      901 tgcccatcgc cttcgagctc ctccacctcg acgaggacac ggaggtccgg gagcagtcca
      961 gcctcttccg ggcggagacg tgcggccggg agatccccgt cgggaacggc ctgcggatga
     1021 cgcttccgtt ctcggcggag cccggcatcg gccactggtc ctactcgggg aacgtggtga
     1081 gggtctcctc gtatcacatg ctctacgact gctccgggga ccccctcggg gtggcctggg
     1141 cggttcgcga ggcgacggtc gtccacagag ccgtcgcgcc cggacccgag gaggcgtccg
     1201 accagaccgc gctccgcgtc gtgttctggc agggggaggc gaccatccgg ttcttcactc
     1261 tgaccggaga gccgctcccg ctgccgctct tcttcaggcc ccctttcccc gggacggact
     1321 ccggagtcag cgagctgtcc ggctcgttcc gtgccgtccg ggcgttcggg gagccgtccc
     1381 gctaggagcg ttcgcgagcg gaaccgcggc cgggaccggc cccgccgcgc cggtccggcc
     1441 cggggcgtag gacgactatc cccgccggtc ccctacttcc agtgcccccg cacccactcc
     1501 tccgtctcgg ggaggcctcc ctgcatgacg aggtagccgt tgtgcggctc gcgcggggtg
     1561 atctcgtgcg cgaccggcgt cgtcatcatc cgcatcacct cggcccgctc gcgcgtgtgg
     1621 ccgaggaccc acgagagctt catgagagcc gtctcgggga tcatgttgtc gagcgggacg
     1681 atgccgaggt ccatcaggtc gcgcccggtg tcgtagacgt acatctgggc gaagccccag
     1741 atcgtctgga cggccatgac gacgtggacg cccgcctccg tcgcccgctt gatcgccggg
     1801 tagagcggct tgttgacgtg gccgaggccg gtcccggcga tgacgatgcc gtggtacccc
     1861 ttctcgacga gggcgtcgac gaggtcgggg ttcatgccgg ggtagtagta gaggatcgtc
     1921 gtccggtcgt cgtagac
//
LOCUS       DAOTEZ010000611         1935 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_52090_length_1935_cov_4.689362, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000611 DAOTEZ010000000
VERSION     DAOTEZ010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52090_length_1935_cov_4.689362"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="P5164_19975"
     CDS             <1..221
                     /locus_tag="P5164_19975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HRY46219.1"
                     /translation="DSVTNVISPTMSYFALILATVQRYDAKAGLGTLIATMLPYTLVF
                     LVSWSLLLMGWIALDLPVGPGAGLRLAP"
     gene            complement(238..1704)
                     /locus_tag="P5164_19980"
     CDS             complement(238..1704)
                     /locus_tag="P5164_19980"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020639932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRY46220.1"
                     /translation="MSDLPFRSATELLAALDRRETSSEELLTLFLSRIERLAKETNAV
                     VTLDAERALAGARRCDEERSRGERRGALHGLPVTVKDSFETEGLLTTSGGTPELMGHV
                     PARDATVVARLKAAGAIVFGKTNLSTQAMDIQTYNAAFGTTNNPWDSGRTAGGSSGGS
                     AVAMSRGLSALEVGSDLGGSIRIPASFCGVFGHRPSYGIVPTRGHIPGPPGSLAAPDL
                     ETAGPIARSADDLDLALRLLAGPDESRGIAWRLELPPPRRRGLSEYRLAAWLDDPAAP
                     VDTALRERLEDVVGELRAAGAEVDTEARPGFALADNARLFMRVLYGSMSSGMPEAQFE
                     KLAARAAALAPDDDSFTARLTRDTVQTHREFDLAREERERQRARWADFFGRYDALLCP
                     AFPTPAFPHDQAPNIARRTLAVNGAPQPYLGTLLGWPALSGLSSLPGTAAPVGFTRDG
                     LPAGIQVVGPYLEDRTAIHVAKLVAEVSGGYVRPPGAA"
     gene            complement(1749..>1935)
                     /locus_tag="P5164_19985"
     CDS             complement(1749..>1935)
                     /locus_tag="P5164_19985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRY46221.1"
                     /translation="IGAFPWRLVDGESCICRILAFVDDDRHAALMAKKERSVRTKWND
                     VAGSPNAWLHEEARKKG"
BASE COUNT          246 a          699 c          728 g          262 t
ORIGIN      
        1 gcgacagcgt cacgaacgtc atctcgccga cgatgtccta cttcgccctc atcctcgcga
       61 ccgtccagcg ctacgacgcg aaggcgggcc tcgggacgct catcgcgacg atgctcccct
      121 acacgctcgt cttcctcgtc tcctggagcc tgctcctgat ggggtggatc gcgctcgatc
      181 tccccgtcgg gccgggggcc gggctgcggc tcgctccgtg acggcgggtc gcgctcgcta
      241 agccgctccc ggcggccgca cgtagccccc cgagacctcc gcgacgagct tcgcgacgtg
      301 gatcgcggtc cggtcctcga gataggggcc gacgacctgg atcccggcgg ggaggccgtc
      361 gcgcgtgaag ccgacgggcg ccgcggtccc cgggagcgag gaaaggccgg agagcgccgg
      421 ccagccgagg agcgtcccga ggtagggctg cggcgcgccg ttcacggcga gcgtccgcct
      481 cgcgatgttc ggagcctggt cgtgcgggaa cgcgggcgtc gggaacgcgg gacagaggag
      541 ggcgtcgtag cgcccgaaga agtcggccca tcgggcgcgc tgccgctccc gctcctcacg
      601 ggcgaggtcg aactcgcggt gggtctggac cgtgtcgcgc gtgaggcgcg cggtgaagga
      661 gtcgtcgtcg ggcgcgagag cggcggcccg ggccgccagc ttctcgaact gcgcctccgg
      721 catcccggaa gacatgctcc cgtagaggac gcgcatgaag aggcgcgcgt tgtccgcgag
      781 ggcgaagccg ggccgcgcct ccgtgtcgac ctccgccccg gcggcacgca gctcgccgac
      841 gacgtcctcg agccgctcgc gaagcgccgt gtcgacgggc gccgccgggt cgtcgagcca
      901 cgccgcgagg cggtactcgg agagcccgcg cctccgcggc ggcgggagct cgagacgcca
      961 ggcgatgccc cggctctcgt cgggtccggc gaggaggcgg agcgcgaggt cgaggtcgtc
     1021 cgcggagcgc gcgatcgggc cagccgtctc gaggtcgggc gccgcgagcg agcccggagg
     1081 gcccgggatg tggccgcgtg tcgggacgat gccgtagctc ggcctgtgcc cgaagacgcc
     1141 gcagaaggag gccgggatcc ggatcgagcc tccgaggtcg ctcccgacct cgagcgccga
     1201 gaggccgcgg ctcatcgcga cggccgagcc gccggaagag ccgcccgcag tgcgcccgga
     1261 gtcccacggg ttgttcgtcg tcccgaacgc cgcgttgtac gtctggatgt ccatcgcctg
     1321 cgtcgagagg ttcgtcttgc cgaagacgat cgctccagcc gccttcaggc gcgctacgac
     1381 cgtcgcgtcg cgcgcgggca cgtgccccat cagctccggc gtcccgccag aggtcgtgag
     1441 gagcccttcc gtctcgaacg agtccttcac cgtcacgggc aggccgtgga gcgccccgcg
     1501 gcgctcgccg cgcgagcgct cctcgtcgca gcggcgcgcc ccggcgagcg cccgctcggc
     1561 gtcgagcgtc acgacggcgt tcgtctcctt cgcgaggcgc tcgatccgcg agaggaagag
     1621 ggtgaggagc tcctcgctcg acgtctcgcg gcggtcgagg gcggcgagga gctccgtggc
     1681 ggagcggaac ggcaggtcgc tcatgcgcgg ctccggggcg cggcgtctcc gccgcgcccc
     1741 gactcccctc agcccttctt ccgcgcctct tcgtggagcc aggcgttcgg agagccggcg
     1801 acgtcgttcc acttcgtccg gacggacctc tccttcttcg ccatgagcgc cgcgtggcgg
     1861 tcgtcgtcga cgaacgcgag gatgcggcag atgcacgact cgccgtcgac gaggcgccac
     1921 gggaaggcgc cgatg
//
LOCUS       DAOTEZ010000612         1932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_52208_length_1932_cov_5.106020, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000612 DAOTEZ010000000
VERSION     DAOTEZ010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52208_length_1932_cov_5.106020"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="P5164_19990"
     CDS             <1..399
                     /locus_tag="P5164_19990"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HRY46222.1"
                     /translation="ALPKIADPPGFAHMVYNYKMLPGPLVNAMALVLPWAELLMGVAL
                     IAGIWRKTAAALVGVLLVVFIAAISFNLLRGNAIDCGCFDVTAANLTREQRFRDMWMV
                     ILRDVGMLVLVAQGLLGASRVEEEGEASAI"
     gene            474..>1932
                     /locus_tag="P5164_19995"
     CDS             474..>1932
                     /locus_tag="P5164_19995"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRY46223.1"
                     /translation="MPTARDLALLKAFDAVLAEGRFLDESIGAVLDTALRFFDAVAVA
                     LLPAGGAPPMSRAGTSIVSTAAEQRLARALEALLSEGREGRVAEAGLVFLCAAVKTGE
                     QVSGAFGVALAQSAGKGMEVDDGIREFARILGRILERERSVATLVKRREEAVALFELA
                     SGALHTLNADEVVRLTAASLQRQMEFDQVRAWRYHPEKGEVESILAQGTSPGTNPGFV
                     PGRRLPIGDDEILVRALAAHGPAFDEEDADDSKGPRRKRRLAIPIEQGETVFGFLTMS
                     RRGAFVLTAQEMRLVKELARLAAGALERARLIEVERRTAEHVTFVNRVHAALSGLNDL
                     ETILAKAVEEAGAGLGLDLCAVQVRSFDGGRPPSSVWTREGGVALPAEGAVPRALVDR
                     LADPDASVLLSDVALAADGPTLVPVPGAAKELPRPLGLVAVPLVSHGEFLGVLAGVGA
                     GRPWAFDPPLLRAFQAVAAEASHAVTSARLLQRERD"
BASE COUNT          240 a          706 c          704 g          282 t
ORIGIN      
        1 gcgctcccga agatcgccga cccgcccggc ttcgcgcaca tggtctacaa ctacaagatg
       61 ctcccggggc cgctggtgaa cgcgatggcg ctcgtcctcc cgtgggcgga gctgctgatg
      121 ggggtcgcgc tgatcgccgg gatctggcga aagacggcgg ccgcgctcgt cggcgtcctg
      181 ctcgtcgtct tcatcgcggc gatctccttc aacctccttc ggggaaacgc catcgactgc
      241 ggctgtttcg acgtgaccgc ggcgaacctg acccgcgagc aacggttccg ggacatgtgg
      301 atggtgatcc ttcgagacgt cgggatgctc gtcctggtgg cccagggcct cctgggggcc
      361 agccgggtcg aggaggaagg agaagcctcg gcgatctgag ccggaggccg ggatgtggct
      421 ggcgatcccg gctatgatcc ggctccgacc aatctccgga gaacgtaacc gccgtgccga
      481 ccgctcgtga cctcgccctg ctcaaggcgt tcgacgccgt cctcgccgag ggccggttcc
      541 tcgacgagtc gatcggcgcg gtcctcgaca ccgcgctccg attcttcgac gccgtcgccg
      601 tcgcgctcct cccggccggc ggcgcgccgc cgatgagccg cgccggcacc tcgatcgtct
      661 cgacggcggc cgagcagcgg ctcgcccggg ccctcgaagc gctcctctcg gagggccgcg
      721 aagggcgggt cgccgaggcg ggcctcgtct tcctctgcgc cgccgtgaag acgggcgagc
      781 aggtgagcgg cgccttcggc gtggctctcg cgcagtcggc gggcaagggc atggaggtcg
      841 acgacgggat ccgcgagttc gcgcggatcc tcgggcggat cctcgagcgc gagcggagcg
      901 tggcgacact cgtgaagcgc cgcgaggagg cggtcgcgct cttcgagctc gcctccggcg
      961 cgctccacac gctgaacgcg gacgaggtcg tccgcctcac ggcggcctcg ctccagcgcc
     1021 agatggagtt cgaccaggtg cgcgcgtggc gctaccaccc cgagaagggg gaggtcgaga
     1081 gcatcctcgc ccaggggacc tctcccggca cgaaccccgg attcgtcccg ggccgccgcc
     1141 ttccgatcgg cgacgacgag atcctcgtcc gggcgctcgc cgcgcacggc cccgcgttcg
     1201 acgaggaaga cgccgacgac agcaagggtc cgcggcgcaa gcgccgcctc gcgatcccga
     1261 tcgagcaggg ggagacggtc ttcggcttcc tgacgatgtc gcggcgcggg gcgttcgtcc
     1321 tgacggccca ggagatgcgc ctcgtgaagg agctcgcccg actcgccgcg ggggcgctcg
     1381 agcgggcgcg cctcatcgag gtcgaacgaa ggacggccga gcacgtcacg ttcgtcaacc
     1441 gggtgcacgc ggcgctctcc ggcctgaacg acctcgagac gatcctggcc aaggccgtgg
     1501 aggaggcggg cgcgggcctc gggctcgacc tctgcgccgt gcaggtccgc tccttcgatg
     1561 ggggcaggcc tccttcgagc gtctggacgc gcgagggcgg ggtcgccctg cccgccgagg
     1621 gggcggtccc gcgcgcgctg gtcgaccgcc tcgccgaccc ggacgcttcc gtcctcctct
     1681 ccgacgtcgc gctcgccgcg gacggcccga cgctcgttcc cgtcccgggc gcggcgaagg
     1741 agctcccgcg ccctctcgga ctcgtcgcag tcccgctcgt gtcgcacggg gagttcctcg
     1801 gcgtcctggc gggcgtgggc gccggccggc cgtgggcgtt cgacccgcct cttctccggg
     1861 cgttccaggc ggtcgccgcg gaggcctccc acgcggtcac gtccgcccgc ctgctgcagc
     1921 gggagcgcga ct
//
LOCUS       DAOTEZ010000613         1929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_52344_length_1929_cov_6.770544, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000613 DAOTEZ010000000
VERSION     DAOTEZ010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52344_length_1929_cov_6.770544"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="P5164_20000"
     CDS             <1..714
                     /locus_tag="P5164_20000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY46224.1"
                     /translation="AAKAAAESARINLSYTPIRAPISGRTGRSSVTVGALVTAYQPVP
                     LATVQQLDPIYVDVAQSSAELLRLQKSFKGGALTKDASWSRVKLILEDGTPYPREGKL
                     KFREVTVDPATGSVTLRMVFPNPDHVLLPGMYVRAVVEEGVLSDALLVPQQGVARTPK
                     GEPYSLVAGKDGKVEQRMLTVGRAIGDRWLVTSGLSAGDPVIVEGAQRVRPGADVKAV
                     PFVPAAPGAAPAGAVPAAK"
     gene            724..>1929
                     /locus_tag="P5164_20005"
     CDS             724..>1929
                     /locus_tag="P5164_20005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRY46225.1"
                     /translation="MSSFFLKRPVFAWVIAIAMMLGGVLAIVKLPVSQYPPIAPPSIT
                     IRAQYTGASAKTVEDSVIQIIESKMTGLDSMLYISSVADSSGGAEVTLTFTPETDPDL
                     AWSKVQNKLQLALPALPDSVQKQGVTVSKSTRNFLLIVGLLSEDGSLTRADLADYVTS
                     QIESPLARVPGVGEVQVFGTGYSMRLWIDPDKLTKYGMTIDDVVAAVRAYNVEVSAGQ
                     FGGAPAVPGQRLNASILIQSLLVTPEEFAAIPLRHDADGSVVKVSDVGRTELGTEIPD
                     IEISYGSLPATAFAIRQEAGANALDTVARIKAKMEELSQFFPPGMKVVYPFDTTPFVR
                     VAIDEVVRTLIEAVVLVFLVMYLFLGNLRATIIPTIAVPVVILGTFGVLALFGYSINM
                     LTMFAMVLAI"
BASE COUNT          287 a          699 c          634 g          309 t
ORIGIN      
        1 gccgcgaagg ccgcggccga gagcgcccgg atcaacctct cctacacgcc gatccgggcg
       61 ccgatctcgg gccggacggg gaggtcgagc gtgaccgtcg gcgccctcgt gacggcctac
      121 cagcccgtcc cgctcgccac ggtccagcag ctcgacccga tctacgtcga cgtggcgcag
      181 tcctcggccg agctcctgcg ccttcagaag agcttcaagg gcggggctct cacgaaggac
      241 gcctcctgga gccgcgtgaa gctgatcctg gaggacggga cgccctatcc gcgcgaaggg
      301 aagctgaagt tccgagaggt caccgtcgac ccggcgaccg gatcggtcac gctccggatg
      361 gtcttcccga accccgacca cgtccttctc cccggcatgt acgtccgcgc cgtcgtcgag
      421 gaaggcgtcc tctccgacgc gctcctcgtc ccgcagcagg gggtcgcccg gacgccgaag
      481 ggggagccgt actccctcgt cgccgggaag gacgggaagg tcgagcagcg gatgctgacg
      541 gtcggccgtg cgatcggcga caggtggctc gtcacctcgg gcctctcggc aggagacccg
      601 gtgatcgtcg agggggccca gcgggtccgt cccggggcgg acgtgaaggc cgttccgttc
      661 gtcccggccg cgccgggagc ggcgccggcg ggcgccgtcc cggcggcgaa gtaagggggc
      721 ctcatgtcgt ccttcttcct gaagcgcccg gtcttcgcct gggtcatcgc gatcgcgatg
      781 atgctcggcg gggtcctggc gatcgtgaag ctcccggtct cccagtaccc gccgatcgcc
      841 ccgccctcga tcacgatccg cgcccagtac accggcgcct cggcgaagac ggtcgaggac
      901 agcgtcatcc agatcatcga gtcgaagatg accggtctcg actcgatgct ctacatcagc
      961 tccgtcgccg actcctccgg cggcgcggag gtgaccctca ccttcacgcc ggagacggac
     1021 cccgacctcg cctggtcgaa ggtccagaac aagctccagc tggctcttcc ggcccttccc
     1081 gactcggtcc agaagcaggg cgtcaccgtc tcgaagtcga cgcgcaactt cctcctcatc
     1141 gtcgggctcc tctccgagga cggcagcctg acgcgcgccg acctcgccga ctacgtcacg
     1201 tcccagatcg agtcgccgct cgcccgggtc cccggcgtcg gcgaggtcca ggtcttcggg
     1261 accggctact cgatgcgcct ctggatcgac ccggacaagc tgacgaagta cgggatgacg
     1321 atcgacgacg tcgtcgcggc ggtgcgggcc tacaacgtcg aggtctccgc cgggcagttc
     1381 ggcggcgcgc ccgccgtccc ggggcagcgg ctgaacgcct cgatcctgat ccagtcgctc
     1441 ctcgtgacgc cggaggagtt cgcggcgatc ccgctccggc acgacgccga cggctcggtc
     1501 gtgaaggtga gcgacgtcgg ccggaccgag ctcgggaccg agattcccga catcgagatc
     1561 tcctacggca gccttccggc gaccgccttc gcgatccgcc aggaggcggg ggcgaacgcg
     1621 ctcgacaccg tcgcccggat caaggcgaag atggaggagc tctcgcagtt cttcccgccg
     1681 gggatgaagg tcgtctaccc gttcgacacg acccccttcg tgagggtcgc gatcgacgag
     1741 gtcgtgagga ccctgatcga agccgtcgtg ctggtcttcc tcgtcatgta cctcttcctc
     1801 gggaacctcc gggcgacgat catcccgacc atcgccgtcc cggtcgtcat cctcggcacg
     1861 ttcggcgttc tcgccctctt cggctactcg atcaacatgc tgacgatgtt cgccatggtc
     1921 ctggcgatc
//
LOCUS       DAOTEZ010000614         1918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_52854_length_1918_cov_5.777241, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000614 DAOTEZ010000000
VERSION     DAOTEZ010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52854_length_1918_cov_5.777241"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="P5164_20010"
     CDS             <1..552
                     /locus_tag="P5164_20010"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRY46226.1"
                     /translation="GRLLLSGVVVNALASAVLLFLLSVGDASDTRGFVFWMLGSLSGA
                     TGDAVFSLAVYSAVGLTVLLPLARAFDALTLGEETAFTLGVSVERAKRIAYVAASLLA
                     AAAVAHAGIIGFVGLLVPHLVRRLTYGHRALLAGSFLAGGVLLVLSDLLARTLFSPNE
                     ISIGAVTALLGAPAFLVLLRRRA"
     gene            549..1352
                     /locus_tag="P5164_20015"
     CDS             549..1352
                     /locus_tag="P5164_20015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY46227.1"
                     /translation="MTPLELRAVFAGYARGKDVLRDVSLTFGEGRVTALLGENGSGKS
                     TLLKVLAGLLPPARGAALLRGRPISEVPRREAARLLGYLPQGFEPFFPATALDVVLLG
                     RTPYLGAFSTPSHRDVAIARAALEEVDAAGLEERDVRSLSGGERQRVYLARALAGEPR
                     VLLLDEPTASLDPRHRFLVLDVLKRLASGGACCVFSTHEVDLAARTADDAVLLARGAV
                     LAAGPVRESVTERSITELFGVAACVTPLADGTPLVTLGAPPAVRAGGRG"
     gene            1365..>1918
                     /locus_tag="P5164_20020"
     CDS             1365..>1918
                     /locus_tag="P5164_20020"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HRY46228.1"
                     /translation="MALWFRKDKAPRALRDMRTATSLGEGHFLKCDGCRETLYAKEVE
                     RNQRVCPKCGFHFRLSVDERLKLLFDDGAFQPLFGDVHPTDPLRFRDSKRYRDRLREY
                     GVRTGRPDAIAAAEGTVEGTKTVVAALDYAFMGGSMGSVVGEVLTRAAEKAAAERAPL
                     VVVTASGGARMQEGILSLMQMAKVS"
BASE COUNT          209 a          762 c          650 g          297 t
ORIGIN      
        1 gggcgcctcc tcctctcggg cgtcgtcgtg aatgccctcg cctccgcggt gctcctcttc
       61 ctcctctcgg tcggcgacgc ctccgacacg cgcggcttcg tcttctggat gctcggctcc
      121 ctctcgggcg cgaccggcga cgccgtcttc tccctcgccg tctactccgc ggtcgggctc
      181 accgtcctcc ttccgctggc ccgcgccttc gacgccctca cgctcggcga ggagaccgcg
      241 ttcaccctcg gcgtctccgt cgagcgggcg aagcggatcg cgtatgtcgc ggcctccctc
      301 ctcgcggccg cggcggtggc gcacgccggg atcatcggct tcgtcgggct cctggtcccc
      361 cacctcgtcc ggcggctcac gtacggccac cgcgccctcc tcgccggctc gttcctcgcc
      421 ggaggggtcc tcctcgtcct ctccgacctc ctcgcgcgga cgctcttctc cccgaacgag
      481 atctcgatcg gcgcggtgac ggcgctcctc ggcgcccccg ccttcctcgt cctcctccgg
      541 aggcgggcgt gacccctctc gagctccgag ccgtcttcgc cggctacgcg cgcggcaagg
      601 acgtcctccg cgacgtgagc ctcacgttcg gggaagggcg cgtcacggcg ctcctgggcg
      661 agaacggctc gggaaagtcg accctcctga aggtcctcgc cggcctcctc ccgccggccc
      721 gaggggcggc cctcctgcgc ggacggccga tctccgaggt gccgcggaga gaggcggcgc
      781 ggctcctcgg ctacctcccg cagggcttcg agccgttctt cccggcgacc gcgctcgacg
      841 tcgtccttct cgggcggacg ccgtacctcg gcgccttctc gacgccgtcc caccgggacg
      901 tcgcgatcgc gcgggccgcg ctcgaggaag tcgatgcggc gggcctcgag gagcgggacg
      961 tgaggagcct ctccggaggc gagcggcagc gggtctacct cgcccgggcg ctcgcgggcg
     1021 agccccgggt cctcctcctc gacgagccga cggcgagcct cgacccgcgc caccggttcc
     1081 tcgtcctcga cgtcctgaag cgcctcgcgt ccggcggcgc ctgctgcgtc ttctccaccc
     1141 acgaggtcga tctcgcggcg cgaaccgccg acgacgcggt cctcctcgcg cgcggagccg
     1201 tcctcgcggc cggaccggtg cgggagagcg tgacggagcg ctcgatcacg gagctcttcg
     1261 gcgtcgccgc ctgcgtcacc ccgctggccg acgggacgcc gctggtcacc ctcggcgccc
     1321 cgcctgcggt ccgcgccggg gggagggggt aagatcccgc ccccatggcc ctctggttcc
     1381 ggaaagacaa ggcgccccgc gccctgcgcg acatgcgtac ggcgacctcg ctcggagagg
     1441 ggcacttcct caagtgcgac ggctgccgcg agaccctcta cgccaaggag gtcgagcgga
     1501 accagcgcgt gtgcccgaag tgcggcttcc acttccgcct ctcggtcgac gagaggctga
     1561 agctcctctt cgacgacggc gcgttccagc ccctcttcgg ggacgtccac ccgaccgacc
     1621 cgctccgctt ccgcgactcg aagcgctatc gcgaccgcct gcgggagtac ggcgtgcgga
     1681 ccggccgccc cgacgcgatc gccgccgcag aagggacggt cgaggggacg aagaccgtcg
     1741 tggccgcgct cgactacgcc ttcatgggag gctcgatggg ctccgtcgtg ggcgaggtcc
     1801 tgacgcgtgc ggccgagaag gccgctgcgg agagggcccc cctcgtcgtc gtcaccgcct
     1861 cgggcggcgc gcggatgcag gaggggatcc tctccctcat gcagatggcg aaggtctc
//
LOCUS       DAOTEZ010000615         1918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_52856_length_1918_cov_4.950081, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000615 DAOTEZ010000000
VERSION     DAOTEZ010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52856_length_1918_cov_4.950081"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1704)
                     /locus_tag="P5164_20025"
     CDS             complement(<1..1704)
                     /locus_tag="P5164_20025"
                     /inference="COORDINATES: protein motif:HMM:NF017400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HRY46229.1"
                     /translation="MWSVTLAAALLCLPARASEILPLEGVRAGMKGYGLTVVRGRDVE
                     KFEVEVVGVVRNPAPGRSVIVVSLSGLGLGESGVVAGMSGSPVYLDGKLAGAVASGWG
                     FSKRAVAGVTPIEAMVSIVPEEPVRPATRAATGPAVADRAALLARLGASSGEERLAEL
                     RRTALDSLPAAMPGPGGATLLSFQASGLPASTARTFRDAFVRLGATEANVELLGAGAA
                     PPPATEPLHSPSRLVPGGALTAYLVRGDLSLGATGTVTEVFPDGRFVAFGHPFLGSGE
                     LELPVAPADVVTVVSSLFQSFKLANGGEALYRLTNDRDTGVGGRMDRPAQTVPITVRV
                     EAGGGPAREFRFEAAAHPKLLPTLAGLVADASMTAADPTARERRLAFRVALDTSAGEI
                     AYEDVATGGRAKEIAVLTTVALAAAVADNDLEDPGVRAVRLRFTSSSDEQRLRLVEAA
                     PVTRTVVPGETLSVAIRLADRRGAESVRMVRLRVPEGTREGRATLVVADGSNATALRM
                     QLDPSEPRTLPELASFVSRIVPGNRLFAGLLVATRGAATRSRTLEALPPSPQRDADLA
                     AA"
BASE COUNT          269 a          720 c          682 g          247 t
ORIGIN      
        1 ggccgccgcc agatcggcgt cgcgctgcgg cgacggcggc agcgcctcga gcgtgcggct
       61 ccgggtcgcg gcccctcgcg tggcgacgag gaggccggcg aagagtcggt ttcccgggac
      121 gatccgggag acgaaggatg cgagctccgg cagcgttcgc ggctcggacg ggtcgagctg
      181 catccggagc gccgtggcgt tgctcccgtc cgcgacgacg agcgtcgccc gcccctcgcg
      241 cgtcccctcg ggcacccgga gccggaccat ccggacgctc tccgctccgc gccggtcggc
      301 gagccggatc gccacggaga gggtctctcc cgggacgacc gtcctcgtca caggggccgc
      361 ctcgacgagg cgcagcctct gctcgtcgga cgagctcgtg aagcggaggc ggacggcgcg
      421 gacgcccgga tcctcgaggt cgttgtcggc gacggccgcg gcgagggcga ccgtcgtcag
      481 gacggcgatc tccttcgccc gccctcccgt cgccacgtcc tcgtacgcga tctccccggc
      541 cgaagtgtcc agcgcgacgc ggaacgcgag ccttcgctcg cgggcggtcg ggtcggcggc
      601 cgtcatcgag gcgtcggcga cgagcccggc gagcgtcggg aggagcttcg gatgcgccgc
      661 tgcctcgaac cggaactctc tcgccggccc gccgccggct tcgacgcgaa ccgtgatcgg
      721 caccgtctgc gcgggccggt ccatccttcc gccgacgccg gtgtcacggt cgttcgtcag
      781 ccggtagagg gcctccccgc cgttcgcgag cttgaacgac tggaagagcg acgacacgac
      841 ggtcacgacg tcggccggag cgaccggcag ctccagctcg ccggagccga ggaacgggtg
      901 accgaacgcg acgaagcggc cgtccgggaa gacctcggtc accgttcccg tcgcaccgag
      961 gctcaggtcc ccgcggacga gataagcggt gagggctccc ccgggcacca gccgtgaggg
     1021 cgagtgaagc ggctctgtgg cgggcggtgg cgcggcaccc gccccgagga gctccacgtt
     1081 cgcctcggtc gcgccgaggc gaacgaaggc gtcacggaac gtccgcgcgg tcgaggccgg
     1141 cagtcccgaa gcctggaagg acagaagagt cgcgcctccc ggcccgggca tcgccgccgg
     1201 cagcgagtcg agagccgtcc ggcggagctc cgcaagccgc tcctcgcccg acgaggcgcc
     1261 cagccgggcg aggagcgccg cgcggtcggc gaccgccggc ccggtcgccg ccctcgttgc
     1321 gggtcggacc ggctcttcgg gaacgatcga gaccatcgct tcgatggggg tcacccccgc
     1381 gaccgcgcgc ttcgagaagc cccagccgga ggcgacggcc ccggccagct tcccgtccag
     1441 gtagaccggg cttccgctca tcccggccac gacccccgac tcccccagcc cgagtccgga
     1501 gagcgaaacg acgatcacgg accggcccgg cgccggattc cggacgacgc ccacgacctc
     1561 cacctcgaac ttctccacgt cgcggccccg cacgaccgtc aggccgtacc ccttcatccc
     1621 ggcacggacc ccttccagcg ggaggatctc cgaggcccgg gcgggcaggc agagaagggc
     1681 cgccgcgagc gtgacgctcc acacgtaacg ctttgacccc cttcgagatt ccgtgctacc
     1741 ttgccccgga tttcggcctg gtcggggcag gagcgacatg acggataccg gcggcgccaa
     1801 catcgttcag ctgttcctca cggcggggtg gacggccaag gccgtgctcc tgatcctcgc
     1861 cctcttctcc ttcggatcgt gggcggtcat cgtcggaaag ctctggagtt tctcacgg
//
LOCUS       DAOTEZ010000616         1914 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_53028_length_1914_cov_5.917160, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000616 DAOTEZ010000000
VERSION     DAOTEZ010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53028_length_1914_cov_5.917160"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1355)
                     /gene="lysS"
                     /locus_tag="P5164_20030"
     CDS             complement(<1..1355)
                     /gene="lysS"
                     /locus_tag="P5164_20030"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRY46230.1"
                     /translation="MPEDTSQQPEQSLAEQVGNRRAARGRIVDLGHPAYPNRFAATSL
                     VSDVVARWGGSDSAALDAEPEPERRIAVPGRVLAIRKMGKAVFLDLSDGRLRVQAYVR
                     KDGVGDGGWALLENLDLGDHVGVTGVVFRTRTGELSVKAESLTFLAKCLRPLPDKWHG
                     LTDVEIKYRQRYVDLIVSEETRRTFEIRARVVSYIRRFFDGRGFLEVETPMMQLIPGG
                     AAARPFVTHHNALDVDLYLRIAPELYLKRLITGGFPKVYEINRNFRNEGISTQHNPEF
                     TMLEFYTAYADARDQMALTEELLAGAAREILGTTQLPWGEETISFEAPFRRLSMKDAV
                     VEYGKDVAGGPIRAEELGTLEGLKWAAERAGVESIERFGDVPGKILAELFETLAEPNL
                     LQPTFVVDYPAEISPLAKTRPDDPRTADRFELFVGRMELANGFSELNDPDEQAEKFRA
                     QV"
     gene            complement(1417..>1914)
                     /locus_tag="P5164_20035"
     CDS             complement(1417..>1914)
                     /locus_tag="P5164_20035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46231.1"
                     /translation="PAVATDPLLGDVASKILYSVDMLQTGAGSGQAAYGSSLSRLRLE
                     PWEVRAARRIQSEDDRAPDQETATNDELFVEGAALRLRIDETAQVLKAPAASGEDAKV
                     LEMAAECLGRAQDLDRRFRQSLEAVGTEGPQERLNELTRSRFRLLRAFSGLWLLHNGR
                     SSGGA"
BASE COUNT          266 a          685 c          665 g          298 t
ORIGIN      
        1 acctgcgcgc ggaacttctc cgcctgctcg tcggggtcgt tcagctccga gaacccgttc
       61 gcgagctcca tccggccgac gaagagctcg aagcggtcgg ccgtccgagg gtcgtccggg
      121 cgggtcttcg cgagcgggga gatctcggcc gggtagtcga cgacgaacgt cggctggagg
      181 aggttcggct cggcgagggt ctcgaagagc tccgccagta tcttccccgg gacgtcgccg
      241 aagcgctcga tcgactcgac ccccgcgcgt tccgcggccc acttcagccc ctcgagcgtt
      301 ccgagctcct ctgcccggat cggccccccc gcaacgtcct ttccgtactc gacgacggcg
      361 tccttcatcg agagcctgcg gaacggcgcc tcgaaggaga tcgtctcctc cccccagggg
      421 agctgcgtcg tcccgaggat ctcccgggcc gccccggcca ggagctcctc ggtgagcgcc
      481 atctggtcgc gggcgtcggc atacgccgtg tagaactcga gcatcgtgaa ctcggggttg
      541 tgctgggtgg agatcccctc gttccggaag ttccggttga tctcgtagac cttcgggaag
      601 ccgcccgtga tcagccgctt caggtagagc tcgggggcga tccggagata gaggtcgacg
      661 tccagcgcgt tgtggtgcgt cacgaacggc cgcgccgcgg ccccgcccgg gatcagctgc
      721 atcatcgggg tctcgacctc gaggaacccc cgcccgtcga agaaccgccg gatgtacgag
      781 acgacgcgcg cgcggatctc gaacgtccgg cgcgtctcct cggagacgat gaggtcgacg
      841 tagcgctggc ggtacttgat ctcgacgtcc gtcagcccgt gccacttgtc ggggagcggc
      901 cggaggcact tcgcgaggaa cgtcagcgac tccgccttca cggagagctc gcccgtgcgg
      961 gtccggaaga cgacgcccgt cacgccgacg tgatcgccga ggtcgaggtt ctcgaggagc
     1021 gcccacccgc cgtcccccac tccgtccttc cggacgtagg cctgcacgcg cagccggccg
     1081 tcggagaggt cgaggaagac cgccttcccc atcttccgga tcgccaggac ccggccggga
     1141 acggcgatcc gcctctccgg ctcgggctcg gcgtcgagcg cggcggagtc gctcccgccc
     1201 cagcgggcga cgacgtccga gacgagggag gtcgcggcga aacgattcgg gtaggcgggg
     1261 tgtccgaggt cgacgatccg gccgcgggcc gccctgcggt ttccgacctg ctcggcgagg
     1321 ctctgctcgg gctgctggga agtgtcttcg ggcatcgggc gccgattctc cgcgccccct
     1381 cggcgcgggt caaacgaaca ccctcagccg cagccgtcag gcccctcccg aagaacgccc
     1441 gttgtggagc agccagaggc ccgagaaggc ccgaaggagg cggaaccggg agcgcgtgag
     1501 ctcgttcagc cgctcctggg gcccttccgt cccgaccgcc tcgaggctct gccggaagcg
     1561 ccggtcgagg tcctgcgccc tccccaggca ttccgccgcc atctcgagga ctttcgcgtc
     1621 ttcccctgac gcggccggcg ccttgaggac ctgggcggtt tcgtcgatcc ggaggcggag
     1681 agcggcccct tcgacgaaga gctcgtcgtt cgtggccgtc tcctggtccg gagcgcggtc
     1741 gtcctctgac tggatcctgc gggccgcgcg gacctcccac ggctcgaggc ggagcctcga
     1801 cagcgagctc ccgtaggccg cctggcccga gccggccccg gtctgcagca tgtcgacgga
     1861 gtagaggatc ttcgacgcga cgtcgccgag cagcgggtcc gtcgcgaccg ccgg
//
LOCUS       DAOTEZ010000617         1913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_53076_length_1913_cov_7.140474, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000617 DAOTEZ010000000
VERSION     DAOTEZ010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53076_length_1913_cov_7.140474"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="P5164_20040"
     CDS             complement(<1..384)
                     /locus_tag="P5164_20040"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRY46232.1"
                     /translation="MNRILVVLLLPLALSSSALGQAPRTGPTPVSTEIVVTAEAAPAD
                     ASSLGVATTVLDRADIERSRATTVANLLRSVPGVDVAQSGGPGGVTSLFLRGANSNSA
                     LVLVDGVKLNSPYFGGVDLSSLGTAN"
     regulatory      complement(486..699)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(760..1629)
                     /gene="asd"
                     /locus_tag="P5164_20045"
     CDS             complement(760..1629)
                     /gene="asd"
                     /locus_tag="P5164_20045"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HRY46233.1"
                     /translation="MSDAAPPLSLRLLGLYPKKAGSAAVGLLARMRLPRALRTPLLGR
                     FAASYRVDLGEAERPLAEYASFLDFFTRRLKAGLRPQEPAVPGGLNSPVDGALIASGR
                     VEQGTLIQAKGLPYRLGELLASDPLAARFEGGAYATLYLSPRDYHRIHVPCPGRVLAV
                     GRVEGELWPVNEASTAFTPGLYVRNRRAYWIAEGTGEDEGLDVAVVMVAATHVGGVVV
                     DARWLGGRTLGRRDRLAVDGLPCAPGDDLGTFELGSTVVLLVGGARASAFDWSRPHGP
                     VRVGERLGALAGA"
     gene            1691..>1913
                     /locus_tag="P5164_20050"
     CDS             1691..>1913
                     /locus_tag="P5164_20050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HRY46234.1"
                     /translation="MSTATETLAGKPLSSNPHLLSWVEECARLCKPDEVVWCDGSEEE
                     KERLTALAVSTKVLIPLNQEKLPGCYLHRS"
BASE COUNT          286 a          670 c          737 g          220 t
ORIGIN      
        1 gttcgcggtg ccgagcgagg agaggtcgac gccgccgaag taggggctgt tcagcttgac
       61 gccgtcgacg aggacgagcg ccgagttcga gttcgccccg cgcaggaaga gcgacgtgac
      121 gccgcccggc ccgccgctct gcgcgacgtc gacgccgggg acgctccgga ggaggttggc
      181 gacggtcgtg gcgcgggagc gctcgatgtc ggcccggtcg aggacggtcg tggcgacccc
      241 gagcgaggag gcgtcggcgg gcgccgcctc ggcggtgacc acgatctccg tcgagacggg
      301 agtcgggccc gtgcggggcg cctggccgag ggcagacgag gagagcgcga ggggaaggag
      361 gagtacgaca aggatgcggt tcaagaggcc tccgtacggc tgcggcctgc cgtcccatcg
      421 gaaggggcgg gaggcgcgcg cggaggcgtc tcccgcgtct tctcgagccc cggagacggc
      481 gcggcgggtt gcgatgggcc tcgggcaggt ctcctggctc ccggatcgtc cgcggaagga
      541 ccccttcccg gaggcgaaac ccgcgtcgcg gatcgctcgc cgccagtggg atgtcgtcct
      601 tcgcgtcccc ggtcacagtg gcgggggccg cgcgggaatc tcacccgctt ccctctctcg
      661 ccccccggag gacgggggac ccgagatccg ttgaaagaac cggtcgcgga ggatagcacc
      721 cgacccgcaa ggggcggcgg cgcgcgggct cgagccgggt caggcgccgg cgagcgcccc
      781 gagccgctcc ccgacccgga ccgggccgtg cgggcggctc cagtcgaaag cagaggcccg
      841 cgcccccccg acgaggagga cgaccgtcga gccgagctcg aacgtcccga gatcgtctcc
      901 cggggcgcac ggaagcccgt cgacggcaag gcggtcgcgg cggccgagcg tccggccccc
      961 gagccagcgc gcgtcgacga cgacgccccc gacgtgcgtc gcggcgacca tcacgaccgc
     1021 gacgtcgagc ccttcgtcct cgcccgtccc ttcggcgatc cagtacgcgc ggcggttcct
     1081 cacgtagagg cccggcgtga aggcggtcga ggcctcgttc accggccaga gctccccctc
     1141 gacgcgcccg acggcgagga cgcgccccgg gcacgggacg tggatccggt ggtagtcgcg
     1201 cggggagagg tagagcgtcg cgtacgcccc gccctcgaag cgcgccgcga gcggatcgga
     1261 ggcgagcagc tcgccgaggc ggtacgggag ccccttcgcc tggatgagcg tgccctgctc
     1321 gacccgcccc gaggcgatga gggcgccgtc gaccggggag ttgagtccgc cggggaccgc
     1381 cggctcctgg gggcggaggc ccgccttcag ccggcgcgtg aagaaatcga ggaacgaggc
     1441 gtactcggcg agcggccgct ccgcctcgcc gaggtcgacg cggtacgacg cggcaaagcg
     1501 ccccaggagc ggcgtacgca gcgcacgcgg cagccgcatc cgagccagga ggccgaccgc
     1561 ggcggagccg gccttcttcg ggtagagacc gaggagccgc agggagaggg gcggggctgc
     1621 gtccgacatg agccgattat gataggggtc ttcccgcgtc cggattgacc gccccgagga
     1681 ggagagcgcg atgagcactg caaccgagac cctggccggc aagccccttt cttcgaaccc
     1741 ccacctcctc tcgtgggtcg aggagtgcgc aaggctctgc aagcccgacg aggtcgtctg
     1801 gtgcgacggc agcgaggagg agaaggagcg cctgacggcc ctcgccgtct cgacgaaggt
     1861 cctgatcccg ctcaaccagg agaagctccc cggctgctac ctccaccgct ccg
//
LOCUS       DAOTEZ010000618         1908 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_53344_length_1908_cov_6.926606, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000618 DAOTEZ010000000
VERSION     DAOTEZ010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53344_length_1908_cov_6.926606"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /locus_tag="P5164_20055"
     CDS             <1..1434
                     /locus_tag="P5164_20055"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRY46235.1"
                     /translation="IYFGKKDSPAALSRDIVAAGAGLCNAEAVRFLAREGPRVVEEVL
                     IGDAGVPFTRTRAGDLDFTREGAHSVARIVHSADATGKAIELALLARVRRERNVRIWT
                     DATAVDLITARHHSTDVQQRYALQDPCLGAYVLDEEGAVHTVLADFTVLATGGVGRLF
                     LHTTNTKHAIGDGLTMAARAGAAVLNLEYVQFHPTTLYHPDAERFLISEALRGEGAVL
                     VNRAGEAFMPRYDRERKDLAPRDVVTRAIVEEMTTRGEPCVYLDLAHHYEGKGRTAIR
                     DRFPTIAATCAKFGIDIATEPIPVVPAAHYFCGGVLADLEGKTTIRNLYAVGEVSCTG
                     LHGANRLASTSLLEGLVWGAHAARSIADRIEKGHTFPPSTFRAIPDWKAPGNARNEDP
                     ALIRQDWTTIRNTMWNYVGIVRTGERLGRAVADIRDLEKRLSRFYHETKISREIVDLF
                     HGVHASHLIAQAALKNPVSRGCHYRRN"
     gene            complement(1585..>1908)
                     /locus_tag="P5164_20060"
     CDS             complement(1585..>1908)
                     /locus_tag="P5164_20060"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRY46236.1"
                     /translation="RADRTNPEELLLGAVVTCFVQTWAIFLAKLRVPVEAPRIDGALE
                     LEADPAGGYRVRRIDLVAHVPAALLAEREADVAKTLSLAEKYCIVSKAVKGEGREVHV
                     TAHPA"
BASE COUNT          288 a          703 c          663 g          254 t
ORIGIN      
        1 atctacttcg gcaagaagga ctcccccgcc gccctttcga gggacatcgt cgcggcgggc
       61 gcgggcctct gcaacgccga ggcggtccgc ttcctcgccc gcgagggacc gcgcgtcgtc
      121 gaggaggtcc tcatcggcga cgcgggcgtc ccgttcaccc ggacccgggc gggggacctc
      181 gacttcaccc gagagggggc gcactccgtc gcgcgaatcg tccactccgc cgacgcgacg
      241 gggaaggcga tcgagctcgc cctcctcgcg cgggtccgtc gcgagcggaa cgtgaggatc
      301 tggaccgacg cgaccgccgt cgacctgatc accgcccgcc accactcgac cgacgtccag
      361 cagcgctacg cgctccagga cccgtgcctc ggcgcctacg tcctcgacga ggaaggggcc
      421 gtccacaccg tcctcgccga cttcaccgtc ctcgcgaccg gaggcgtcgg gcggctcttc
      481 ctccacacga cgaacacgaa gcacgccatc ggggacggcc tgacgatggc cgcgcgcgcc
      541 ggcgcggccg tcctgaacct cgagtacgtc cagttccacc cgacgaccct ctaccacccg
      601 gacgccgagc gattcctcat ctccgaggcg ctccggggcg agggggcggt cctcgtgaac
      661 cgggccggcg aagccttcat gccgcgctac gaccgggaga ggaaggacct cgccccgcgc
      721 gacgtcgtca cccgcgcgat cgtggaggag atgacgaccc gcggcgagcc gtgcgtctac
      781 ctcgacctcg cgcaccacta cgaggggaag ggtcggacgg cgatccgcga ccgcttcccg
      841 acgatcgccg ccacgtgcgc gaagttcggg atcgacatcg ccaccgagcc gatcccggtc
      901 gtcccggctg cccactactt ctgcggcggc gtcctcgccg acctcgaggg gaagacgacg
      961 atccggaacc tctacgccgt cggcgaggtg agctgcacgg gcctccacgg cgcgaaccgc
     1021 ctcgcctcga cgtcgctcct cgaggggctc gtctgggggg cgcacgcggc gcggtcgatc
     1081 gccgaccgga tcgagaaggg tcacacgttc ccgccgtcca cgttccgcgc gatccccgac
     1141 tggaaggccc ccgggaacgc ccggaacgag gacccggctc tcatccggca ggactggacg
     1201 acgatccgca acacgatgtg gaactacgtc gggatcgtcc ggacgggcga gcggctcggc
     1261 cgggccgtgg ccgacatccg cgacctcgag aagcgcctct cgcgcttcta ccacgagacg
     1321 aagatctccc gcgagatcgt cgacctcttc cacggcgtcc acgcgagcca cctgatcgcg
     1381 caggccgcgc tgaagaaccc ggtctcgcgc gggtgccact accggaggaa ttagaggtcc
     1441 gcgtggagga tgcgccggga ccgccggcca acgcctccgg ctcttcgggc tttcttgcgg
     1501 acatggatcg gggaggctcg ccacgagcct ccccgcggcg gctgcgccgc ctcccctcgg
     1561 gtggggatat ggaaggctga agcgtcaggc ggggtgggcg gtgacgtgga cctcgcgtcc
     1621 ctcgcccttc accgccttcg agacgatgca gtacttttcg gcgagagaga gggtcttcgc
     1681 gacgtcggcc tcgcgctcgg cgaggagcgc ggccgggacg tgcgcgacga ggtcgatccg
     1741 gcggacgcgg tagcccccgg ccgggtcggc ctcgagctcc agagcgccgt cgatgcgcgg
     1801 cgcctcgacg gggacgcgga gcttcgcgag gaagatcgcc caggtctgga cgaagcaggt
     1861 gacgacggcg ccgaggagga gctcctccgg gttcgtccgg tcggcgcg
//
LOCUS       DAOTEZ010000619         1899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_53759_length_1899_cov_5.456616, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000619 DAOTEZ010000000
VERSION     DAOTEZ010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53759_length_1899_cov_5.456616"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1355
                     /locus_tag="P5164_20065"
     CDS             <1..1355
                     /locus_tag="P5164_20065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HRY46237.1"
                     /translation="EEISQRLATSRDPKELAALWAGWHEVGAGLRKDYARYVELGNAG
                     AKELGFADMGAMWRSKYDMPPDAYAKELDRLWEQVKPLYVSLHAYVRMKLRETYGKDV
                     VPEKGPIPAHLLGNMWAQQWGSLYPILAPKDADQGYDLTKILVEKKTDARQMVRYGEG
                     FFTSLGFETLPKTFWERSLFTRPRDREVVCHASAWCVDWVDDLRLKMCIEINAEDFAT
                     VHHELGHNVYQRAYNRQPVLFRDSANDGFHEAIGDVIALSLTPEYLKKVGLLTKTPPP
                     GKDVGYLLNMALEKIAFLPFGLLIDKWRWQAFSGEVPEARWNEAWWDLKLKYQGVAPP
                     VARSEADFDPGAKYHIPAGVPYSRYFLAHVLQFQMHRALAKTAGCTDPIHTCSIYGNK
                     EAGAKLIRMLEMGQSQPWPDALEALTGQREMDATAVLDYFAPLQKWLDEQTKGQPKGW
                     "
     gene            1361..1759
                     /locus_tag="P5164_20070"
     CDS             1361..1759
                     /locus_tag="P5164_20070"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRY46238.1"
                     /translation="MTPRLPPPTFEYLNGEVLEQAPGRVRCLFRPTEEMTNPWGAVQG
                     GILAAFFDDTMGPAVVSIANGRGFTTVSLNVTYLRAAKPGEAVVCEAIVVKHGRTQAY
                     AEATLTRESDGELLARATSVNLFLEGPKKG"
BASE COUNT          318 a          676 c          635 g          270 t
ORIGIN      
        1 tcgaggagat ctcccagcgc ctcgcgacga gccgcgaccc gaaggagctc gccgcgctct
       61 gggccggctg gcacgaggtc ggggcggggc tccggaagga ctacgcccgc tacgtcgagc
      121 tcgggaacgc cggcgcgaag gagctcggct tcgccgacat gggcgcgatg tggcggagca
      181 agtacgacat gccgcccgac gcctacgcga aggagctcga ccggctctgg gagcaggtga
      241 agcccctcta cgtctcgctc cacgcctacg tccggatgaa gctgcgcgag acctacggca
      301 aggacgtcgt ccccgagaag gggccgatcc cggcgcacct cctcgggaac atgtgggccc
      361 agcagtgggg cagcctctac ccgatcctcg ccccgaagga cgccgaccag ggctacgacc
      421 tgacgaagat cctcgtcgag aagaagaccg acgcgcggca gatggtgcgc tacggggagg
      481 gcttcttcac gtctctcggc ttcgagaccc tcccgaagac gttctgggag cgctcgctct
      541 tcacccgccc gcgcgaccgc gaggtcgtct gtcacgcgag cgcctggtgc gtcgactggg
      601 tcgacgacct gcggctgaag atgtgcatcg agatcaacgc ggaggacttc gcgaccgttc
      661 accacgagct cgggcacaac gtctaccagc gcgcctacaa ccgccagccc gtcctcttcc
      721 gcgacagcgc gaacgacggc ttccacgagg cgatcggcga cgtcatcgcc ctctctctta
      781 cgcccgagta cctgaagaag gtcgggctcc ttacgaagac gcctcctccc ggcaaggacg
      841 tcggctacct cctcaacatg gcgctcgaga agatcgcctt cctgccgttc ggcctcctca
      901 tcgacaagtg gcggtggcag gccttctccg gcgaggtccc ggaggcgagg tggaacgagg
      961 cgtggtggga cctcaagctg aagtaccagg gggtcgcccc gcccgtcgcg cgctccgagg
     1021 ccgacttcga cccgggcgcg aagtaccaca tcccggcggg cgtcccctac tcgcgctact
     1081 tcctcgccca cgtcctccag ttccagatgc accgcgccct cgcgaagacg gccggttgca
     1141 ccgatccgat ccacacctgc tcgatctacg ggaacaagga agccggggcg aaactcatcc
     1201 ggatgctcga gatggggcag agccagccgt ggcccgacgc gctcgaggcc ctcaccggcc
     1261 agcgcgagat ggacgcgacg gccgtcctcg actacttcgc gccgctccag aagtggctcg
     1321 acgagcagac gaaggggcag ccgaagggct ggtagagggg atgacgccga ggctcccgcc
     1381 cccgacgttc gagtacctca acggcgaggt cctcgagcag gcgcccggga gggtccgctg
     1441 cctcttccgc ccgacggagg agatgacgaa cccctggggc gccgtccagg gcgggatcct
     1501 cgccgccttc ttcgacgaca cgatgggacc cgcggtcgtc tcgatcgcga acggccgcgg
     1561 gttcaccact gtctcgctca acgtgaccta cctgagggcc gcgaagcccg gcgaggccgt
     1621 cgtctgcgag gcgatcgtcg tgaagcacgg ccgcacccag gcctacgccg aggcgaccct
     1681 cacccgcgag tccgacggcg agctcctcgc gcgcgcgacc tccgtcaacc tcttcctcga
     1741 gggaccgaag aaggggtagg ggaccggggt accggggtac cggagagacg agcttcgagg
     1801 tcacgtcgat tccgcggggg gccgcgtccg gggcggggca tctcttcggc tccttctgcg
     1861 cgcggccgac ccgcttccga ctttgctccg gccgggcgg
//
LOCUS       DAOTEZ010000620         1878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_54793_length_1878_cov_5.566100, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000620 DAOTEZ010000000
VERSION     DAOTEZ010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54793_length_1878_cov_5.566100"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1521
                     /locus_tag="P5164_20075"
     CDS             <1..1521
                     /locus_tag="P5164_20075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46239.1"
                     /translation="EVTCRFCGAPALLERRLRKVEPEVEGAPLRLFLDAASAEAGGDP
                     SATPWVRSKGFRQSVVTRTLCPGCGEALEWKDDAERVDCPSCGTDCRLERRLQAPPLD
                     PARGVPRRRTAHENATADDDDPRTEQLVWRVVNEEDPRRRFALALRFEEWLFVNATAA
                     RLLPSLLRVLGEAEPALQVAIADLVGKLLCEGSVELRNATLRATEPFAFERAVPPTLL
                     FALGLGDGTSLKLLLDAAELAHRRGDDDAATTALLAVNWIFQRNYPHHAVMGEILLHR
                     MLYLSGPPLAFALLLARRRVTGTGFHYSAETLLSFLDEAALERPSLVPELGRCFYVGL
                     PKDEGEARARMAFFATLRTKAAREASLREHLLPPEEAPDALYAEMAALFLPLASDAAL
                     AGAAELALKRLVDAPREVPACVHALVAERGRSLPDEVQRAYLRAVPGTPHLSWDDVGY
                     GEVEREEPLAPELQSLLDRWNEDLRRAADEVDAARAAFRERKDELSGLDVPLFDQG"
     gene            complement(1651..>1878)
                     /locus_tag="P5164_20080"
     CDS             complement(1651..>1878)
                     /locus_tag="P5164_20080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011556528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRY46240.1"
                     /translation="DYVKNWKTPTLVIHGQKDFRVVYAQGLSTFTALQRRGIPSKLLI
                     FPDENHWVLKPANSLQWHDTVLGWLDEWLKR"
BASE COUNT          247 a          708 c          658 g          265 t
ORIGIN      
        1 gaggtgacgt gccgcttttg cggcgcgccg gccctcctcg agagacgcct gaggaaggtc
       61 gagccggagg tcgagggagc gccgctccgt ctcttcctcg acgccgcctc ggccgaggcg
      121 ggaggcgacc cgtcggcgac gccctgggtg aggtcgaagg gcttccggca gtccgtcgtg
      181 acgcggacgc tctgccccgg ctgcggcgag gcgctcgagt ggaaggacga cgccgagcgg
      241 gtggactgcc cgtcctgcgg caccgactgc cgtctcgaga ggcgcctgca ggcgcccccc
      301 ctcgacccgg cgcgcggcgt cccgcggcgg cgcacggcgc acgagaacgc gacggcggac
      361 gacgacgacc cgcgaacgga gcagctcgtc tggcgcgtcg tgaacgagga ggaccccagg
      421 cgccggttcg cgctcgcact ccgcttcgag gagtggctct tcgtgaacgc gaccgccgcg
      481 cgcctccttc cctcgctcct ccgagtcctg ggcgaggcgg agccggccct ccaggtggcg
      541 atcgccgacc tggtcgggaa gctcctctgc gaagggagcg tcgagctccg caacgcgacg
      601 ctccgggcca cagagccttt cgccttcgag cgggccgtcc cgccgacgct cctcttcgcg
      661 ctcggcctcg gcgacggaac ctccctgaag ctcctcctcg acgccgccga gctcgcccac
      721 cggcgcggcg acgacgacgc ggcgacgacg gcgctcctgg ccgtgaactg gatcttccag
      781 cggaactacc cgcaccacgc cgtgatggga gagatcctcc tccaccggat gctctacctg
      841 agcgggccgc cgctcgcgtt cgcgctcctc ctcgcgcgcc gccgcgtcac cggaaccggc
      901 tttcattact ccgcggagac gctcctctct ttcctcgacg aggccgccct cgagcgcccc
      961 tccctcgtcc ccgagctcgg ccgctgcttc tacgtcgggc tcccgaagga cgagggcgag
     1021 gcccgcgcgc ggatggcgtt cttcgcaacg ctccgcacga aggccgcgcg cgaggcctcg
     1081 ctccgcgagc acctcctgcc gccggaggag gcgccggacg ccctctacgc ggagatggcc
     1141 gcgctcttcc ttccgctggc ctcggacgcc gcgctcgccg gagccgcgga actcgcgctg
     1201 aagcggctcg tcgacgcccc gcgcgaggtc ccggcctgcg tccatgcgct cgtcgcggag
     1261 cgcgggcgaa gccttcccga cgaggtgcag cgggcgtacc tgcgcgcggt gcccggcaca
     1321 ccccacctct cgtgggacga cgtgggatac ggagaggtcg agcgcgagga gcccctcgcg
     1381 ccggagctgc agtcgctcct cgaccgctgg aacgaagacc tccgccgcgc cgcggacgag
     1441 gtcgacgcgg cgcgcgcggc attccgcgag cggaaggacg agctctcggg gctcgacgtc
     1501 ccgctcttcg accagggctg agaaccgcct ccccaggatc gttcgcggcc gctcgtcgag
     1561 cacctcggaa acgagagcac ccggctctcg ccgggcgctc tcggaccatc gagaggcgcg
     1621 gcgccagggg tggcgccgcg cctcactccg tcacctcttc agccactcgt ccagccagcc
     1681 gagcacggtg tcgtgccact ggagggagtt cgcgggcttg aggacccagt ggttctcgtc
     1741 ggggaagatc aggagcttgc tcgggatgcc gcggcgctgg agggcggtga acgtcgagag
     1801 cccctgcgcg tagacgactc ggaagtcctt ctggccgtgg atgacgagcg tcggggtctt
     1861 ccagttcttc acgtagtc
//
LOCUS       DAOTEZ010000621         1875 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_54949_length_1875_cov_5.496154, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000621 DAOTEZ010000000
VERSION     DAOTEZ010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54949_length_1875_cov_5.496154"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="P5164_20085"
     CDS             complement(<1..391)
                     /locus_tag="P5164_20085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRY46241.1"
                     /translation="MNRSGRPSFPAAPLLGLLALLAPAPAGAQPMMPSPTREVGWEQN
                     MGAQLPLDAKFTDETGKSVRLGDYFGKRPVILSLAYYECPMLCGIALEGLARSLKGFT
                     LTPGADFEVVTLSFSPTEGPELAKAKKA"
     gene            complement(388..819)
                     /locus_tag="P5164_20090"
     CDS             complement(388..819)
                     /locus_tag="P5164_20090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46242.1"
                     /translation="MSDHPRPQHAPAEFDSEIHVKAIFGVLAGLALLVALAFGGMIAF
                     SGFLKSRSIARDPEPLPLAAANAPRPRPRAALQADPTADMTKFAREEEAAVSSYAWVD
                     RAAGVAQIPVSRALEIVAERGLPVPPPLPEAGPAPAAGGSK"
     gene            complement(812..>1875)
                     /locus_tag="P5164_20095"
     CDS             complement(812..>1875)
                     /locus_tag="P5164_20095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46243.1"
                     /translation="LFRSYLTGYMWMLGLAAGALGVMLLHHVTRGAWGVVIRRILEAA
                     ASPLTLGFAAVAFLPIALGTHSLYEWTHADVVAGDAILTHKKPWLNEPAFLGRSAAYL
                     VLWIVLAAVFSRLSARQDETGDRRIALSMQRWGAFGVVAFILSMTFAAFDWMMSLTPH
                     WFSTIYGLYVIIGQAVAAMALVALAGLLLGEGPSPALPLQPRHLHDHGKLLFAFVCVW
                     AYFGYSQFIIIWSGNLPEETTFYTSRLNGAWKVTTIVLVLLHYALPFALLLSRNRKRS
                     AKALAPVAVLLLFARWLDLHWLVAPAFSANAFTLHWLDLAAPLALGGIWLRLFTLGLK
                     ARPLLPANEPFLKEALAHE"
BASE COUNT          307 a          555 c          786 g          227 t
ORIGIN      
        1 tcgccttctt ggccttcgcg agctcggggc cttcggtcgg gctgaagctg agcgttacga
       61 cctcgaagtc ggcgcccggg gtcagggtga agcccttcag gctccgggcg agcccctcga
      121 gagcgatgcc gcagagcatg gggcactcgt aataggcgag ggagaggatg accgggcgct
      181 tcccgaagta gtcgccgagg cggacggact tgccggtctc gtcggtgaac ttcgcgtcga
      241 gcgggagctg cgcccccatg ttctgctccc agccgacctc gcgcgtgggc gagggcatca
      301 tcggctgggc tcctgcggga gccggcgcga ggagggcgag gaggccgagc aggggcgccg
      361 ctgggaatga ggggcggccg gagcggttca cttcgatcct cctgcggcgg gggccgggcc
      421 ggcctcgggg agcggcggcg ggacgggaag cccgcgctcg gcgacgatct cgagcgcgcg
      481 cgagacgggg atctgcgcga cgccggcggc ccggtcgacc caggcgtacg aggagaccgc
      541 cgcctcctcc tcgcgcgcga acttcgtcat gtcggccgtg gggtcggcct gcagcgcggc
      601 tcgggggcgc ggacgcggcg cgttcgccgc ggcgagcggg agcggctcgg ggtcccgcgc
      661 gatcgagcgg gacttcagga agccgctgaa ggcgatcatc ccgccgaagg cgagggcgac
      721 gaggagcgcg aggcccgcga ggacgccgaa gatcgccttg acgtggatct cggaatcgaa
      781 ctccgccggc gcgtgctgcg ggcggggatg gtcactcatg ggcgagcgcc tccttgagga
      841 acggctcgtt cgcggggagg agcggccggg ccttcagccc gagcgtgaag aggcggagcc
      901 agatcccgcc gagggcgagc ggcgcggcga ggtcgagcca gtggagcgta aaggcgttcg
      961 cggagaacgc cggtgcgacg agccagtgga ggtcgagcca ccgggcgaag aggaggagga
     1021 cggcgacggg ggcgagcgcc ttcgcgctgc gcttgcggtt cctcgagagg agcagggcga
     1081 acgggagggc gtagtggagg aggacgagga cgatcgtcgt gaccttccag gccccgttca
     1141 ggcgggaggt gtagaaggtc gtctcctccg gcaggttccc cgaccagatg atgatgaact
     1201 gcgagtagcc gaagtaggcc cagacgcaga cgaaggcgaa gaggagcttg ccgtggtcgt
     1261 ggaggtgccg cggctggagc gggagggcgg gggaagggcc ctcgccgagg aggagccccg
     1321 cgagggcgac gagcgccatc gccgcgacgg cctgaccgat gatgacgtag aggccgtaga
     1381 tcgtggagaa ccagtgtggc gtcagcgaca tcatccagtc gaacgccgcg aaggtcatcg
     1441 agaggatgaa ggcgacgacg ccgaacgcgc cccagcgctg catcgagagg gcgatccgcc
     1501 ggtcgcccgt ctcgtcctgc cgggccgaga ggcgggagaa gacggcggcg aggacgatcc
     1561 agaggacgag gtaggccgcg gagcgcccga ggaaggcggg ctcgttcagc cacggcttct
     1621 tgtgcgtgag gatcgcgtcg cccgcgacga cgtcggcgtg cgtccactcg tagagggagt
     1681 gggtcccgag ggcgatgggg aggaacgcga ccgcggcgaa gccgagcgtg agggggctgg
     1741 cggcggcttc gaggatccgg cggatgacga cgccccaggc gccgcgcgtg acgtggtgca
     1801 ggagcatgac gccgagggcc ccggcggcga ggccgagcat ccacatgtag ccggtgaggt
     1861 aggagcggaa gagct
//
LOCUS       DAOTEZ010000622         1869 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_55263_length_1869_cov_5.303749, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000622 DAOTEZ010000000
VERSION     DAOTEZ010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55263_length_1869_cov_5.303749"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="P5164_20100"
     CDS             complement(<1..720)
                     /locus_tag="P5164_20100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013108498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY46244.1"
                     /translation="MRAAYALARREVVRFLRQPSRIVGAFAPPLLAWLLLGAGFGASF
                     RAPGGASYLGWFFPGTVVLVVLFAAIFSTISVIEDRREGFLQAVVVSPVSPLAVAAGK
                     IAGGTLLALAQGGALLLLAPVLGYTPSMAALVPALLVLAFVALALTGLGLAIAWMLES
                     TQGFHAIMNLFLIPMWFLSGSFFPSEGAPAWLRAAMAVNPLTYGVAALRHALDPGASG
                     LPSSGLSVAVTFAFALGAVLVA"
     gene            complement(717..1715)
                     /locus_tag="P5164_20105"
     CDS             complement(717..1715)
                     /locus_tag="P5164_20105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY46245.1"
                     /translation="MSAPPLVEVDGLSRSYGDRLALDGVSFGVAEGELFALLGPNGGG
                     KTTLFKLLSTLLPPSSGTFRVDGLSPVRDLRGLRRRLGVVFQSPSLDRKLTVLENLLV
                     HGAVLGLGGAALRARAAELLARMGVADRAGDRVETLSGGLARRVEIAKALLHAPRVLL
                     LDEPSTGLDPLARRSLRALLRELAGGGTTVLLTTHLFDEAEEAGRIGLLDRGRLVALG
                     SPADLKRDVGGEVLVLATESGDEAERLASDVAAALGARAEDSVPSRRVGALGATVRIE
                     SLDAPSLVPSLAAAFAGRFRSLSFGAPSLEDVFVDRTGHPFDGDAADAPASAGRGA"
BASE COUNT          308 a          597 c          804 g          160 t
ORIGIN      
        1 ggcgacgagg acggcgccga gggcgaaggc gaacgtgacg gcgacggaga ggccggacga
       61 cgggaggccg gacgccccgg ggtcgagggc gtggcggagg gcggcgacgc cgtaggtgag
      121 gggattgacg gccatcgcgg cccgcagcca cgcgggggct ccctcggagg ggaagaacga
      181 gcccgacagg aaccacatcg gtatcaggaa gagattcatg atggcgtgga agccctgcgt
      241 cgactcgagc atccaggcga tggcgaggcc gaggcccgtg agcgcgagcg ccacgaacgc
      301 gaggacgaga agggccggaa cgagcgccgc catcgacggc gtgtatccga ggacgggcgc
      361 gaggaggagg agtgcccctc cctgcgcgag ggcgaggagc gtgcctccgg cgatcttccc
      421 ggccgcgacg gccagcgggg agaccggcga gacgacgacg gcctggagga acccctcgcg
      481 ccggtcctcg atgacggaga tcgtcgagaa gatcgccgcg aagaggacga cgaggacgac
      541 cgtgccggga aagaaccagc cgaggtagct ggcgccgccc ggcgcgcgga acgaggcgcc
      601 gaagccggcg ccgaggagga gccaggcgag gagcggcggg gcgaaggcgc cgacgatccg
      661 gctcggctgg cggaggaagc ggacgacctc gcgccgggcg agcgcgtacg cggccctcac
      721 gcgcccctcc ccgcggaggc cggggcgtct gcggcgtcgc cgtcgaaggg gtggccggtg
      781 cggtcgacga agacgtcctc gagcgaggga gcgccgaacg agagcgagcg aaagcggccg
      841 gcgaaggcgg cggcgagcga ggggacgagc gacggggcgt cgagcgactc gatccgcacc
      901 gtcgcgccga gggcgccgac gcggcgggag gggacggagt cctccgcccg cgcgccgagc
      961 gccgcggcga cgtcggaggc gaggcgctcg gcctcgtcgc cggactccgt cgcgaggacg
     1021 aggacctcgc cgccgacgtc gcgcttgagg tcggccggcg agccgagcgc gacgaggcgg
     1081 ccgcgatcca ggaggccgat ccggcccgcc tcctcggctt cgtcgaagag gtgtgtcgtc
     1141 aggaggacgg tcgtcccgcc gcccgcgagc tcgcggagaa gcgcgcggag gctccggcgc
     1201 gcgagcgggt cgaggccggt cgagggctcg tcgaggagga ggaccctcgg ggcgtggagg
     1261 agcgccttgg cgatctcgac gcggcgggcg aggccgccgg agagcgtctc gacgcggtcg
     1321 cccgcccggt ccgcgacgcc catcctggcg aggagctcgg cggcgcgagc gcggagcgcc
     1381 gcgccgccga ggccgaggac ggctccgtgg acgaggaggt tctcgaggac ggtcagcttc
     1441 cggtcgaggc tcggcgactg gaagacgacg ccgaggcggc ggcggaggcc gcggaggtcg
     1501 cggacggggg agaggccgtc gacgcggaac gtgcccgacg agggaggaag gagcgtcgag
     1561 aggagcttga agagggtcgt cttgccgccg ccgttcggtc cgaggagggc gaagagctcc
     1621 ccctccgcga cgccgaagga gacgccgtcg agggcgagcc ggtcgccgta gctccggctg
     1681 agcccgtcga cctcgacgag ggggggagcg ctcatccgcc cgagacgcgg tcggcgagga
     1741 ggaggacgac gatcgcgggg agccaggcga tggaggcgag gaacatcgac ttcgccgcgc
     1801 ggtccgtcgt ctcgcggaag aacgtcaccg ccgtccccgc gaaccatccc gagaggagga
     1861 gcgcgccgg
//
LOCUS       DAOTEZ010000623         1868 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_55327_length_1868_cov_6.668505, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000623 DAOTEZ010000000
VERSION     DAOTEZ010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55327_length_1868_cov_6.668505"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /gene="ccsA"
                     /locus_tag="P5164_20110"
     CDS             <1..555
                     /gene="ccsA"
                     /locus_tag="P5164_20110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRY46246.1"
                     /translation="PVLAGTPWLAIHVPIIMVGYAVLALGVVVAHMQIGVAAFGRGRG
                     ALVEKLYELLYWYMHVGSIFLLAGILTGSVWAASSWGRYWGWDPKEVWSLVAFLAYMA
                     ILHLKVDKVIGTFGAAAISILAFQTILMTYLGVNYVLNAGMHSYGMGDSPIVKWMIVV
                     ALVEVVFLAVCGVAFRDRKRIVEA"
     gene            710..>1868
                     /locus_tag="P5164_20115"
     CDS             710..>1868
                     /locus_tag="P5164_20115"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HRY46247.1"
                     /translation="MKATRIRAAEALGLPLAAAVLVTLVAAAGWVDSAELLVRDALLR
                     TRPRSAAASVAAVVVDEEAIGAVGPWPWERERLAALVERIGAAGAAGVAVDLLLAETR
                     PGDEALAAALAARPSVLAAAPDGTRRWLLPGAGLSAASGVAHVSFAVDRDGVVRGFLA
                     TRQLDGLSLGAMPLAAARLLRPEMPVPVGASIRPGFRSAGVVPTWSAADVLSGTLPED
                     ALRGRVCLVGSVAAGIGDRVVSPLSARGTPDPGVLVQAAATESILTGDLYDRLPPIVA
                     GGLTGVLTWGATALRRRVAPGRALAAAAVPLLAVPAGIVSLLWAGWELPLLATAFGGL
                     AATAGPELWRLAADRRRAAVAVRRIGELEALAATLESERKDDAEARRVLAHE"
BASE COUNT          198 a          656 c          705 g          309 t
ORIGIN      
        1 ccggtcctcg cggggacgcc gtggctcgcg atccacgtcc cgatcatcat ggtcggctac
       61 gccgtcctcg ccctcggcgt cgtcgtcgca cacatgcaga tcggcgtcgc cgcgttcggc
      121 agggggcgcg gcgcgctcgt cgagaagctc tacgagctcc tctactggta catgcacgtc
      181 gggtcgatct tcctcctcgc ggggatcctg accggctccg tctgggccgc ctcgtcgtgg
      241 ggccgctact ggggctggga cccgaaggag gtctggtcgc tcgtcgcgtt cctcgcctac
      301 atggcgatcc tccacctgaa ggtcgacaag gtgatcggga cgttcggcgc ggcggcaatc
      361 agcatcctcg cgttccagac gatcctgatg acgtacctcg gcgtgaacta cgtcctgaac
      421 gcgggtatgc attcctacgg catgggcgac tcgcccatcg tgaagtggat gatcgtcgtc
      481 gccctcgtcg aggtcgtctt cctcgccgta tgcggggtcg ctttccgcga ccggaagagg
      541 atcgtggagg cgtgatatgg gcgggaatgg ctgagccggg cgggttggcg cgggggaatc
      601 gggcctcgac cgccccttgc cgaatgcacg caggcgctcg ttcgagctgc gcgagaaacc
      661 cgtacccctc taaggcggtt ctaatgttcg cggacgtacc ctcctcgggg tgaaggcgac
      721 tcggattcgg gcggctgaag ccctcggcct gccgttggcg gctgcggtcc tcgtgacgct
      781 cgttgccgct gcgggctggg tcgattccgc cgagctcctc gttcgggacg cgctcctgcg
      841 gacccggccg aggtcggcgg cggcgtccgt cgcggcggtc gtcgtcgacg aggaggcgat
      901 cggcgccgtc gggccatggc cctgggagcg ggagcgcctg gccgccctcg tggagcggat
      961 cggggcggcc ggggccgccg gcgtggcggt cgacctcctg ctcgcggaga cccgcccggg
     1021 cgacgaggcc ctcgccgccg cgcttgcggc gagaccgtcg gttcttgccg ccgcgccgga
     1081 cgggacgagg cgatggcttc tccccggtgc cggcttgagc gcggcttcgg gggtggcaca
     1141 cgtcagcttc gccgtcgatc gcgacggcgt cgttcgcgga ttcctcgcga cccgccagct
     1201 cgacggcctt tccctcggcg cgatgccctt ggcggcggcc cggctgctgc ggccggagat
     1261 gcccgtgccg gtcggggctt cgatccgacc cggcttccgt tccgcgggcg tggtgccgac
     1321 ctggagcgcc gccgacgtgc tgagcgggac gctgccggag gacgcgctcc gaggcagggt
     1381 ctgtctcgtc ggctcggtcg cagccgggat cggcgaccgc gtcgtctctc ccctgtcggc
     1441 ccgcggtacg cccgatccgg gcgtcctcgt ccaggcggcg gcgaccgagt cgatcctcac
     1501 gggggacctg tacgaccggc tccctccgat cgtggccggg gggctcacgg gcgtcctgac
     1561 gtggggagcg acggctctgc ggcggcgggt cgcgccgggg cgggcccttg ccgcggccgc
     1621 tgtcccgctg ctcgcggttc cggccggaat cgtgtcgctt ctctgggccg ggtgggagct
     1681 gccgctcctc gcgacggcgt tcggcggcct cgccgcgacg gcgggccccg agctgtggcg
     1741 cctcgcagca gaccgccgcc gggccgcggt cgcggtccgg cgcatcggcg agctcgaagc
     1801 gctcgccgca acgctcgaga gcgagcgaaa ggacgacgcg gaggcgcgcc gcgtcctggc
     1861 gcacgagc
//
LOCUS       DAOTEZ010000624         1863 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_55589_length_1863_cov_3.744469, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000624 DAOTEZ010000000
VERSION     DAOTEZ010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55589_length_1863_cov_3.744469"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="P5164_20120"
     CDS             <1..328
                     /locus_tag="P5164_20120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46248.1"
                     /translation="VSGAEATPASDQWAIAATGVELLTGVRLKFERPDLTLKSLVEEV
                     VSVVTTVASGVPVTYARSLAKAMAMDPEDRWVNVLALRRALDRSTGGRIPGDDDVQFD
                     VLREQT"
     gene            complement(361..741)
                     /locus_tag="P5164_20125"
     CDS             complement(361..741)
                     /locus_tag="P5164_20125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46249.1"
                     /translation="MGEANGAARQDDVRPPFRFAGRSFVVASNRPARELRALEREIEA
                     RGGSIHPFLNAVTEVLVVGDLAGMDSGAHWAREQVRRGTIYRDRWGHLELVSEEELRA
                     VLEESRSGRPNGPPLGDPSFRERA"
     gene            968..1126
                     /locus_tag="P5164_20130"
     CDS             968..1126
                     /locus_tag="P5164_20130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46250.1"
                     /translation="MDVLVLILLLVTGFGAPSTDGASETSSVTTPSTTTETTLSDGGD
                     TVRKQPIG"
     gene            complement(1139..>1863)
                     /locus_tag="P5164_20135"
     CDS             complement(1139..>1863)
                     /locus_tag="P5164_20135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46251.1"
                     /translation="LRARAAYYAGSALWGAGRSEAALSRLLAAREAFAADGQDPWVGR
                     AEGAIGLVHFSEARFREALHAFDAALERLDPDVDPGPWEAIQQNRAGILMNLGRLSEA
                     RSAFGQALELAVRHGLSAAATTVRVNLLNLGLEEGTFEDVRARGKKLVAFCDREGLSV
                     DAYYARLALAEAQAALGSYGEVRALVEAIRRDAPLEVRDDPDATLLLDRLDAGDNEMA
                     GRLRRLRHYLSGRDRFEAAKRA"
BASE COUNT          271 a          645 c          666 g          281 t
ORIGIN      
        1 cgtctccggc gcggaggcga cccccgcgtc ggaccagtgg gcgatcgcgg cgaccggcgt
       61 ggagcttctg accggggtgc ggctcaagtt cgagaggccc gacctgacgc tcaagtcgct
      121 cgtcgaggag gtcgtgtcgg tcgtcacgac ggtcgcctcg ggagtcccgg tcacctacgc
      181 ccgttccctc gcgaaggcga tggccatgga cccggaagac cgctgggtaa acgtcctcgc
      241 cctccgcagg gccctcgacc ggagcaccgg ggggcgcatt ccgggagacg acgacgtgca
      301 gttcgacgtc ctccgggagc agacgtagcg accatcggga ggcgaagagg ccattggggc
      361 tcaggctcgt tcacggaacg aggggtctcc gaggggcggc ccgttcgggc ggccgctccg
      421 gctttcctcg aggacggccc ggagctcttc ctcgctgacg agctcgaggt gcccccagcg
      481 gtcccgatag atcgttcccc gacggacctg ctcccttgcc cagtgcgccc ccgagtccat
      541 cccggcgagg tctcccacga cgagcacctc ggtcacggcg ttcaggaacg gatggatcga
      601 tccgccccgg gcctcgatct cacgctcgag cgcgcgaagc tcccgggcgg gccgattgct
      661 cgcgacgacg aacgaccgcc cggcgaaccg gaaggggggc cggacgtcgt cctgtcgtgc
      721 agccccattc gcttctccca tggacctccc cctttcgggc ggccagtccg gggacggacc
      781 ccggccgtgg catcccccta ctcctgatga gggatctcgg ggggggcgag tgcccctctc
      841 cccgtaactt tttttctttt ttttcgaggg aggggggcag tttcgggggt cgaatcccct
      901 cataggtaat gaatgggggc ttggcccctg agcgacgacc ggccgaaacg gccggaatgg
      961 aggattcatg gacgttctgg tgctgattct tcttctcgtc accggcttcg gggcgccgtc
     1021 gacggacggc gcgtcggaga cctcgtcggt cacgacaccg tccaccacga cggaaacgac
     1081 gctcagcgat gggggagata ccgtgcggaa gcagccgatc ggttgacccg cgcctccgtc
     1141 aggctcgctt cgccgcctcg aaccggtctc ggccggagag gtaatggcgg agccgccgaa
     1201 gccggccggc catctcgttg tccccggcgt cgagccggtc caggagaagg gtcgcgtcgg
     1261 gatcgtcgcg gacctcgagc ggggcgtccc tgcgtatcgc ttcgaccagg gcccggacct
     1321 cgccgtagct gccgagcgcc gcctgtgcct cggccagcgc gagcctcgcg tagtaggcgt
     1381 cgaccgagag cccctcgcgg tcgcagaagg cgacgagctt cttcccgcgg gcgcgaacgt
     1441 cctcgaaggt gccctcctcg aggccgaggt tcagcagatt gacccggacc gtcgtcgcgg
     1501 ccgccgagag gccgtggcgg acggcgagct cgagggcctg cccaaaggcc gaccgggcct
     1561 cggagagacg gccgaggttc atgaggatcc cggcgcggtt ctgctggatc gcctcccagg
     1621 gacccgggtc cacgtccggg tcgaggcgct cgagggcggc gtcgaaggcg tgcagggcct
     1681 cgcggaaccg ggcctcgctg aagtgaacga gtccgatcgc gccttcggcc cggccgaccc
     1741 aggggtcctg accgtccgcg gcgaaagctt cgcgggccgc gaggaggcgg ctcaacgcgg
     1801 cctcggatcg acccgccccc cagagcgctg agccggcgta ataggcggca cgggcgcgca
     1861 gca
//
LOCUS       DAOTEZ010000625         1856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_55943_length_1856_cov_6.331483, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000625 DAOTEZ010000000
VERSION     DAOTEZ010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55943_length_1856_cov_6.331483"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..792
                     /locus_tag="P5164_20140"
     CDS             46..792
                     /locus_tag="P5164_20140"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HRY46252.1"
                     /translation="MTNKSPSGALERLSHLQELLLEARTKAERRNRTPEHLVHVEAAW
                     QDFRRKRQDVVSRREQAEARKKELDEQVAGYAEQLKKSEAKRKAVKGQHEYTALLTEV
                     DHAQREIRAREDELLQVEETLALAKSEEAALAATSGEEEAGYEEQMSEWRAEQAKLAE
                     EIAAAEKAATELRASVDKRLLGVFDRIAKMRHGVAVARVAMVANQTAACSSCNVRLRP
                     QLLSDLRLSREILQCESCKRILYWDGSGLP"
     gene            789..1187
                     /locus_tag="P5164_20145"
     CDS             789..1187
                     /locus_tag="P5164_20145"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HRY46253.1"
                     /translation="MTAGRLVLHFDGACRGNPGPSSWGVWSPSGIAKSGLVGRTTNNV
                     AEWSGFLAALDAAIESGAADVEIRADSELVVKQWNGVYRVKAPHLLPFVAQAKEKARR
                     IPRLRVVHVRREENGEADRLANEALDGAKG"
     gene            1184..1846
                     /locus_tag="P5164_20150"
     CDS             1184..1846
                     /locus_tag="P5164_20150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRY46254.1"
                     /translation="MRRTAEIAANLAGVRSRIEAAARRAGRDPSSVTLLAVTKTKPLE
                     DLLAAWEAGVRHFGENRVQEAEAKFPHLPSGAGKHLIGPIQSNKANKAARLADVLHAV
                     DSADLARRLARAAGNEGKRLAVFVEVNTGGEETKAGVSPAEVPALVAEVLALPALDLR
                     GLMSIPPPGETRAHFVALRRLAESLGLAELSMGMSDDFEAAIEEGSTIVRVGTALFGS
                     RS"
BASE COUNT          288 a          664 c          668 g          236 t
ORIGIN      
        1 acgggcgccg ctgggcgccc gttcactttt tcagggagac gttctttgac caacaaatcg
       61 ccgtccggcg ctctcgagcg cctctctcac ctccaggagc tcctcctcga agcccgcacc
      121 aaggcggaga ggcgcaacag gacgcccgag cacctcgtcc acgtcgaggc ggcctggcag
      181 gacttccggc ggaaacggca agacgtcgtg tcgcggcgcg agcaggccga ggcccgcaag
      241 aaggagctcg acgagcaggt cgcgggctac gcggaacagc tcaagaagtc cgaggcgaag
      301 cgcaaggccg tcaagggcca gcacgagtac acggcgctcc tcaccgaggt cgaccacgcg
      361 cagcgcgaga tccgggcgcg cgaggacgag ctcctccagg tcgaggagac gctggccctg
      421 gcgaagtccg aggaagccgc cctcgccgcc acctccgggg aggaggaggc gggctacgag
      481 gagcagatga gcgagtggcg cgccgagcag gcgaagctcg ccgaggagat cgcggcggcc
      541 gagaaggccg cgacggagct ccgcgcctcc gtggacaagc ggctcctcgg cgtcttcgac
      601 cggatcgcga agatgcggca cggcgtcgcc gtggcgcgtg tcgcgatggt cgcgaaccag
      661 acggcggcct gctcctcgtg caacgtccgg ctccgccccc agctcctctc cgacctgcgc
      721 ctctcgcgcg agatcctcca gtgcgagagc tgcaagcgga tcctctactg ggacggttcc
      781 ggcctgccgt gacggccggc cgcctcgtcc tccacttcga cggcgcctgc cgcggcaacc
      841 ccggaccctc ctcgtggggc gtctggtcgc cgtcgggcat cgcgaagtcg ggtctcgtcg
      901 ggcgtacgac gaacaacgtc gcggagtgga gcggcttcct cgcggcgctc gacgccgcga
      961 tcgagtcggg ggccgccgac gtcgagatcc gggccgacag cgagctcgtc gtcaagcagt
     1021 ggaacggcgt ctatcgggtg aaggcgccgc accttctccc gttcgtcgcg caggcgaagg
     1081 agaaggcccg gaggattccg cgcctccgcg tcgtccacgt tcggcgggag gagaacgggg
     1141 aggccgaccg gctcgcgaac gaggcgctcg acggggcgaa ggggtgaggc gcaccgcgga
     1201 gatcgccgcg aacctcgccg gcgtgcggtc ccggatcgag gcggccgccc gcagggccgg
     1261 gcgcgacccc tcctccgtca cgctcctcgc cgtgacgaag acgaagccgc tcgaggacct
     1321 cctcgcggcg tgggaggcgg gggtccgcca cttcggcgag aaccgggtcc aggaggcgga
     1381 ggccaagttc ccgcaccttc cgtcgggcgc cggaaagcac ctgatcggtc cgatccagtc
     1441 aaacaaggcg aacaaggccg cgcggctcgc cgacgtcctt cacgccgtcg actcggccga
     1501 cctcgcccgg cgcctcgccc gcgccgcggg gaacgaggga aagcgcctcg ccgtcttcgt
     1561 cgaggtgaac accgggggcg aggagacgaa ggccggcgtc tcccctgcgg aagtccccgc
     1621 gctcgtcgcc gaggtcctgg ccctcccggc gctcgacctc cgcggcctca tgtcgatccc
     1681 gccgccgggc gagacgcgcg cgcacttcgt cgccctccgg cgcctcgccg agtcgctcgg
     1741 cctcgcggag ctgtcgatgg ggatgagcga cgacttcgag gcggcgatcg aggagggatc
     1801 aacgatcgtg agggtcggga cggccctttt cggctcccgc tcctgaccct gctacg
//
LOCUS       DAOTEZ010000626         1848 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_56382_length_1848_cov_4.725042, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000626 DAOTEZ010000000
VERSION     DAOTEZ010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56382_length_1848_cov_4.725042"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1420)
                     /locus_tag="P5164_20155"
     CDS             complement(<1..1420)
                     /locus_tag="P5164_20155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY46255.1"
                     /translation="MPYSLVAEGVSFVLPDGTPLLESIDLSLPRGFTALVGPNGSGKS
                     TLLALLAGALRPARGRVLRSGSVALLPQSTAPDRGGVARDLLEARDAPEWLAARVLAE
                     VGLDGLDLDRPAATLSGGETTRLRLARLLLGEPETLLLDEPTNHLDGEARQAVHDLVR
                     SFRGAVLVATHDRALLARADRIAELRSGRLRSFGGGWEGFLEAWRAEREAAARAVRSA
                     EQELEAVRRAAHEAAERQAHRAARGRREAARGGMPKIVAGGLKRAAQVSAGRLKGIHG
                     DRIEAARSALAKARAAAPDDPSVAFDLGATPVPPRKRIVDAEGLNVRLPGGWLWAAPL
                     SFHVVGPERIRLRGANGAGKSTLFALLAGRAPDAGTLRVGAARVALLDQDAAVLGSEG
                     TLAGTLRRLAPTRPEHERRLLVGRFGFVQEAAEKPVAALSGGERIRAGLAALLASEQA
                     PELLLLDEPSNHLDLAGLEAVAA"
BASE COUNT          224 a          673 c          755 g          196 t
ORIGIN      
        1 cggcggcgac cgcctcgagc ccggcgaggt cgaggtggtt cgacggctcg tcgaggagga
       61 ggagctccgg cgcctgctcc gacgcgagga gcgccgcgag accggcgcgg attcgctcgc
      121 cgcccgacag tgccgcgacc ggcttctccg cggcctcctg gacgaagccg aaacgcccga
      181 cgaggagacg ccgctcgtgc tcggggcggg tcggcgcaag gcgccggagc gtcccggcga
      241 gggtcccctc gctcccaaga acggcggcgt cctggtcgag gagcgcgacg cgcgcggcgc
      301 cgacgcggag cgtcccggcg tcgggcgcgc ggccggcgag gagggcgaag agcgtcgact
      361 tcccggcgcc gttggccccg cgcagccgga tccgctccgg gccgacgacg tggaacgaga
      421 ggggcgccgc ccagagccag ccacccggga ggcggacgtt gagcccctcg gcgtcgacga
      481 tccgctttcg gggcggcacc ggcgtcgcgc cgaggtcgaa ggcgaccgac gggtcgtccg
      541 gtgcggcggc gcgggccttc gcgagggcgg atcgcgccgc ctcgatccgg tccccgtgga
      601 ttccctttag ccgcccggcg gagacctgcg cagcccgctt cagcccgccc gcgacgatct
      661 tcggcatccc gccccgcgcc gcctcccggc ggccccgcgc ggcgcggtgc gcctgccgct
      721 cggccgcctc gtgcgcggcc cgccggacgg cctcgagctc ctgctccgcg gagcggacgg
      781 cccgcgcggc cgcctcgcgc tcggcccgcc acgcctcgag gaagccttcc cagccgccgc
      841 cgaacgaccg gaggcgaccg gagcgaagct ccgcgatccg gtcggcgcgg gcgaggagcg
      901 cgcggtcgtg cgtcgcgacg aggaccgcgc cccggaagct ccggacgagg tcgtggacgg
      961 cctgccgcgc ctccccgtcg aggtggttcg tcggctcgtc gaggaggagc gtctccggct
     1021 cgccgaggag gagccgcgcg aggcggaggc gcgtcgtctc gccgccggag agagtcgcgg
     1081 cgggacgatc gaggtcgagg ccgtcgaggc cgacctcggc gaggacgcga gcggcgagcc
     1141 actccggggc atcccgggcc tcgaggaggt cccgggcgac gcccccgcgg tcgggcgctg
     1201 tcgactgggg gaggagggcg acgcttccgc tccgcaggac gcggccgcgg gccggccgaa
     1261 gcgctcccgc gagaagcgcg aggagcgtgc tcttgccaga gccgttcggg ccgacgaggg
     1321 ccgtgaagcc ccgcgggagg gagaggtcga tcgattcgag gagcggcgtc ccgtcgggca
     1381 ggacgaagga gacgccttcg gcgacgaggg aatacggcat gaagacctcg ctcgggagcc
     1441 gggagcggcg ggctgctcga ccggcaggtg cgttcggggg aggcgggtct tcagcttctc
     1501 atcgggcccg cggggcgggc ggagagatgg tacgacgcgc ggcgcgcggc ggaaagaggg
     1561 ccccgcgccc gggccacctc gaagcgtgcg cgggaacaaa caggtgcggc gcggcgttct
     1621 cgcttccgct gcggggcgga gcgcggcgcg accttcggac cggtcctgcg gtccggcgcg
     1681 tcggccgacg ccccgccgga aggagcgcga atgtccgagc cggtctggca tctcctcgtc
     1741 ggcggccacc aggtgggtcc cgtctccccg gacgacgtcc gggcgagggt gagggacggc
     1801 agcgccgacc gggcgacgct cgccttcggc ccgggcctct ccgcctgg
//
LOCUS       DAOTEZ010000627         1847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_56423_length_1847_cov_7.904576, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000627 DAOTEZ010000000
VERSION     DAOTEZ010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56423_length_1847_cov_7.904576"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1847)
                     /gene="purL"
                     /locus_tag="P5164_20160"
     CDS             complement(<1..>1847)
                     /gene="purL"
                     /locus_tag="P5164_20160"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HRY46256.1"
                     /translation="LWSEHCSYKSSKVFLRDLPTKGPRVVQGPGENAGAVDIGDGLAV
                     VFKMESHNHPSFIEPYQGAATGVGGILRDVFTMGARPIAVLDPLFFGDPSAPRMHGLV
                     DGVVRGVGGYGNCIGIPTVGGSTFFHPSYDGNILVNVMAVGLVRHERIFKAKASGIGN
                     PVLYAGSKTGRDGIHGASMASDVFDDEKAQRRPTVQVGDPFTEKLLLEAVLEVLEGDA
                     VVAIQDMGAAGLTSSSFEMCARGDVGMRLDLDRVPMRESGMTPYELMLSESQERMLLV
                     TKKGREREVIEVFERWGLDVAEIGEVTAEPRMVLLFRGEVAADLPVAPLVDEAPVYRR
                     PWTLAEPLPPLEGAIDLSGAPDETAALLALLASPNLCSKAWIWRQYDRSVRTNTVAGP
                     PGDAAVLRVKGTKKGLALTADVNPRYCFADPMRGAAHAVAEAALNVACTGARPLAITD
                     CLNFGNPERPEIMGQLAAAVKGLSLACRALETPVVSGNVSLYNETDGESILPTPTVGM
                     VGLLEDVARAVPHAFRAEHDVVALLGETRDELGRSEYLATVLGRDEGPCPLLDLLAVR
                     SMVDLLVDLAADGLLSSAHDVSEGGLAVALAEAAIPNGLGADLNVETALL"
BASE COUNT          265 a          639 c          684 g          259 t
ORIGIN      
        1 aggagcgccg tctcgacgtt caggtcggcg ccgaggccgt tcgggatcgc cgcctcggcg
       61 agcgcgacgg cgagccctcc ctccgacacg tcgtgcgcgg aggagaggag gccgtctgcc
      121 gcgaggtcca cgaggaggtc gaccatcgac cggaccgcga ggaggtcgag gagcgggcag
      181 gggccctcgt cgcggccgag gacggtcgcg aggtattcgc ttcggccgag ctcgtcgcgc
      241 gtctctccga ggagggcgac gacgtcgtgt tccgcgcgga aggcatgcgg caccgcccgg
      301 gcgacgtcct cgaggaggcc caccatcccg acggtcggcg tcgggaggat cgactcgccg
      361 tccgtctcgt tgtagaggga gacgttcccc gagacgacgg gcgtctcgag ggcgcgacag
      421 gcgagggaga ggcccttcac cgccgcggcg agctggccca tgatctccgg acgctcgggg
      481 ttgccgaagt tgaggcagtc ggtgatcgcg agcggtcgcg cgcccgtgca ggcgacgttc
      541 agcgccgcct cggctaccgc gtgcgcggcc ccgcgcatcg ggtcggcgaa gcagtagcgc
      601 gggttgacgt ccgccgtcag cgcgagcccc ttcttcgtcc ccttcacgcg gaggacggcg
      661 gcgtcccccg gcgggccggc gaccgtgttc gtcctcacgc tccggtcgta ctgccgccag
      721 atccacgcct tgctgcagag gttcggcgaa gcgagaaggg cgaggagggc cgccgtctcg
      781 tcgggggcgc cggagaggtc gatcgcccct tcgagcggcg ggagcggctc ggcgagcgtc
      841 caggggcggc ggtagacggg cgcctcgtcg acgagcggcg cgacggggag gtcggccgcg
      901 acctcgcccc gaaagaggag gaccatccgc ggctcggcgg tcacctctcc gatctcggcc
      961 acgtcgaggc cccacctctc gaagacctcg atcacctcgc gctcgcgccc cttcttcgtg
     1021 acgaggagca tccgctcctg cgactcggag agcatcagct cgtagggggt catccccgac
     1081 tcgcgcatcg gcacccggtc gaggtcgagg cgcatcccga cgtcgccgcg ggcgcacatc
     1141 tcgaacgagg acgaggtgag ccccgcggcc cccatgtcct ggatcgcgac gacggcgtcg
     1201 ccttcgagga cctcgaggac cgcctcgagg aggagcttct cggtgaacgg gtcgccgacc
     1261 tgcaccgtcg ggcggcgctg cgccttctcg tcgtcgaaga cgtcggaggc catcgaggcg
     1321 ccgtggatcc cgtcgcggcc cgtcttcgag ccggcgtaga ggaccgggtt gccgattccc
     1381 gaggccttcg ccttgaagat cctctcgtgg cggacgaggc cgaccgccat gacgttcacg
     1441 aggatgttgc cgtcgtacga cgggtggaag aacgtcgacc cgccgacggt cgggatcccg
     1501 atgcagttcc cgtagccgcc gacgccgcgg acgacgccgt cgaccaggcc gtgcatccgg
     1561 ggagccgaag ggtcgccgaa gaagagcgga tcgaggacgg cgatcggcct cgcccccatc
     1621 gtgaagacgt cgcgcaggat gccgccgacg cccgtcgccg cgccctggta cggctcgatg
     1681 aacgacgggt ggttgtggga ctccatcttg aagacgaccg cgaggccgtc gccgatgtcg
     1741 acggcgccgg cgttctcgcc cggcccctgg acgaccctcg gccccttcgt cgggaggtcg
     1801 cggaggaaga ccttcgacga cttgtaggag cagtgctcgc tccagag
//
LOCUS       DAOTEZ010000628         1844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_56561_length_1844_cov_4.884293, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000628 DAOTEZ010000000
VERSION     DAOTEZ010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56561_length_1844_cov_4.884293"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..882)
                     /locus_tag="P5164_20165"
     CDS             complement(55..882)
                     /locus_tag="P5164_20165"
                     /inference="COORDINATES: protein motif:HMM:NF019997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HRY46257.1"
                     /translation="MSPPVSIRWAELLADAVSRPGQILEAYRLFHGYSLGNQLAVMFQ
                     CRERRLAPGPIATLQRWNELKRRVRAGEKAIVLCMPVSVRVKDDERSRRSVPEGEAPR
                     LKTIFVWKPRWFVLAQTDGEPLTNAVEIPSWDEARALEALAVQRIPFDLPDGNVQGFA
                     HGRSVAVSPIAALPAKTLFHELGHVLLNHTAERGDDRPDLPKTLTEAEAEAVALLCLE
                     ALHLPGAEYARGYIQSWYGAGNPLPEHSAQRIFKAADTILRAGRSEPAPGAAERGAA"
     gene            complement(972..1580)
                     /locus_tag="P5164_20170"
     CDS             complement(972..1580)
                     /locus_tag="P5164_20170"
                     /inference="COORDINATES: protein motif:HMM:NF036314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46258.1"
                     /translation="MPGSSFSLPAKRTCPGVVLTPTSVCSACYADERKRYRWRTVKLA
                     QDRRLTWTVHALTSGRFAPVVTELISRRDERHFRLHDSGDFFSPPYVDAWYEIALTLP
                     EVSFWAPTRSWAINGQVRPDDDPLLVSLRRLAFLPNVTVRPSALLIDSDPPEIPGLHA
                     GSSVTTELARATCPKYLRSPPSCGDCRHCWAEPLKPVVYLKH"
BASE COUNT          297 a          560 c          719 g          268 t
ORIGIN      
        1 tgacgcccag atgggcgccg gccggcgaga gcgggaggga cgagaggtcg agcctcatgc
       61 ggcgcctcgc tccgcggcac cgggtgcggg ttcgctgcga ccggcacgga ggatcgtgtc
      121 ggcggccttg aagatccgct gggcggagtg ctccgggagg gggttgcccg cgccgtacca
      181 cgactggatg tagccgcggg cgtactcggc gcccgggagg tggagcgcct cgagacagag
      241 gagggcgacc gcctcggcct ccgcttccgt gagcgtcttc gggagatccg gcctgtcgtc
      301 gccgcgctcg gcggtgtggt tgaggaggac gtggccgagc tcgtggaaga gggttttggc
      361 cgggagcgcg gcgatggggc ttaccgcgac ggagcgcccg tgggcgaagc cctggacgtt
      421 gccgtcgggc aggtcgaaag ggatgcgctg gaccgcgagg gcctcgaggg cgcgcgcctc
      481 gtcccacgat gggatttcga cggcgttcgt gaggggctct ccgtcggtct gggccaggac
      541 gaaccaccgc ggcttccaga cgaagatcgt cttcaaccgc ggggcctcgc cttcgggcac
      601 cgaccggcgg ctccgctcgt cgtccttcac ccggacgctc accggcatgc acaggacgat
      661 ggccttctcg ccggcccgca cgcgccgttt gagctcgttc catcgctgga gtgtggcgat
      721 cggccctggg gcgaggcgtc gttcgcggca ctggaacatg accgcgagct ggtttccgag
      781 gctgtagccg tggaagaggc ggtaggcctc gaggatctgg ccggggcggg agaccgcgtc
      841 agcgaggagc tccgcccagc gaatcgagac gggtggagac atcagggacc tccttgcgtg
      901 tcaggtcccg gacgccctcg cgcccgggac ggtgtttcgt ccggggcacg acgtcgagat
      961 agagggcaag ctcagtgctt gaggtagacc accggtttca agggctcggc ccagcagtgg
     1021 cggcagtcgc cacaggacgg cgggctcctg aggtacttcg ggcaggtggc gcgcgcgagt
     1081 tcggtcgtga cggaactccc cgcgtgcaga ccaggaatct cgggagggtc gctgtcgatc
     1141 aggagcgcgc tcgggcgaac cgtgacgttc ggcagaaatg cgagacggcg aagggagacg
     1201 aggagcggat cgtcgtcggg gcggacctgg ccgttgatcg cccagctcct cgtcggcgcc
     1261 cagaacgaga cctcgggaag cgtgagggcg atctcgtacc aggcgtcgac gtagggtggg
     1321 ctgaagaagt cgccggaatc gtggagtcgg aaatggcgtt cgtctcgacg ggagatgagc
     1381 tccgtgacga ccggggcgaa gcgtccggac gtgagggcgt gaacagtcca ggtgagccgc
     1441 ctgtcctggg cgagcttcac ggttcgccac ctgtagcgct tccgttcgtc ggcgtaacag
     1501 gcgctacaaa cggacgtcgg cgtgagaacg accccggggc aggtgcgctt ggccgggaga
     1561 ctgaaggagc tccccggcat cgcgtccgta cgcgtgagca gacacatcag gacctcctcg
     1621 atggtttccc gggcgcctcg gcgccctcgg gcggccgacg tctcgaccga aggtcccaag
     1681 caaaggagcg gacctattcg ggtgcagaga cgccagccgc cagagagctc agagggccgg
     1741 ggagcacccg ccggccgagg aggtcccgga aaccgggaga ccggcgggtg ccgaagcgag
     1801 ttgggaagga gctgcggccg tgccgacgcc agaaagagac caca
//
LOCUS       DAOTEZ010000629         1843 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_56617_length_1843_cov_7.435123, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000629 DAOTEZ010000000
VERSION     DAOTEZ010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56617_length_1843_cov_7.435123"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1843)
                     /locus_tag="P5164_20175"
     CDS             complement(<1..>1843)
                     /locus_tag="P5164_20175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRY46259.1"
                     /translation="VPAVAIDVFKQSKANTVGVVEAVTKEIEKLRGELPPSVEIQLVR
                     DGSVMIKESVHDVTTTLIIGAILTVLIVFLFLNSWRSTVITGLTLPISVISSFIVMYF
                     LGMTLNVMTLMALSLAIGLLIDDAIVVRENIVRHLEEGKDHMTAARVGTAEIGLAVLA
                     TTMSIVAVFVPVAFMKGIMGRFFFQFGITVAFAVLVSLFVSFTLDPMLSSRWVDPDVE
                     RLGKRHAVARALDRFNEWFDRTADRYKALIAWALDHRKAVLAFATVAFVAGIGLFGTL
                     ESEFMANPDQAEFQVKFKTAPDASFEETRGRMEAVLAELKKMPEVQHTYASIGAGDMG
                     TVRDARVYVKLVDKSDRTKHAKVLASEARGRIAGIAGIIPSVEMQSDEFAEKPVMVSI
                     RGEEIPLLKKYAAELKKAVYGIPGIVDVETTLELDLPEYRLVVDRERAADTGLSTPAI
                     ARTVGALVGGQAVTTYEDEEGEAVDVRVRLPLEMRRDVGQIGDLRIAVPGGRGGAPAL
                     VPLSELVKAERATTPSEISRRDLSREVLLTANLDGLPLGTAAQKIKEAAAKIDMAPGY
                     QVFFPGEAERMEESFGYMAEALLLAVVFVYLILAAQFESFIDPLSIML"
BASE COUNT          301 a          612 c          645 g          285 t
ORIGIN      
        1 agcatgatcg agagcgggtc gatgaacgac tcgaactgcg ccgcgaggat gaggtagacg
       61 aagacgaccg cgaggaggag cgcctcggcc atgtacccga agctctcctc catccgctcg
      121 gcctcgcccg ggaagaagac ctggtagccc ggcgccatgt cgatcttcgc cgcggcctcc
      181 ttgatcttct gcgccgccgt cccgagcggc aggccgtcga ggttcgcggt gaggaggacc
      241 tcgcgcgaga ggtcgcgccg ggagatctcc gagggggtcg tcgcccgctc ggccttcacg
      301 agctcggaga gcgggacgag cgccggggcg ccgccccggc cgcccgggac ggcgatccga
      361 aggtctccga tctggccgac gtcgcgcctc atctcgagcg ggaggcggac cctcacgtcg
      421 accgcctccc cctcctcgtc ctcgtaggtc gtgacggcct ggccgccgac gagcgcgccg
      481 acggtccggg caatggccgg cgtcgagagg ccggtgtcgg ccgcccgctc gcggtcgacc
      541 acgaggcggt actcggggag gtcgagctcg agcgtcgtct cgacgtcgac gatgccgggg
      601 atgccgtaga cggccttctt cagctccgcg gcgtacttct tgaggagcgg gatctcctcg
      661 ccgcggatcg agaccatgac cggcttctcg gcgaactcgt cgctctgcat ctccacggac
      721 gggatgatcc cggcgatccc ggcgatccgg ccgcgcgcct cggacgcgag gaccttcgcg
      781 tgcttcgtcc gatccgactt gtcgacgagc ttcacgtaga cccgcgcgtc cctcacggtt
      841 cccatgtcac ccgcgccgat cgaggcgtag gtgtgctgca cctcgggcat cttcttcagc
      901 tcggcgagga cggcctccat ccggccgcgc gtctcctcga acgaggcgtc cggggccgtc
      961 ttgaacttga cctggaactc cgcctggtcg gggttcgcca tgaactccga ctcgagcgtc
     1021 ccgaagagac cgatgccggc gacgaaggcg acggtcgcga aggcgaggac ggccttgcgg
     1081 tggtcgagcg cccaggcgat cagggccttg tagcggtcgg ccgtccggtc gaaccactcg
     1141 ttgaaccggt cgagggctcg cgccacggcg tgccgcttcc cgagccgctc gacgtcgggg
     1201 tcgacccagc gcgaggagag catcgggtcg agcgtgaagg agacgaagag ggagacgagg
     1261 accgcgaagg cgacggtgat gccgaactgg aagaagaagc gccccatgat ccccttcatg
     1321 aaggcgaccg ggacgaagac ggcgacgatc gacatcgtcg tcgccaggac ggcgaggccg
     1381 atctcggccg tgccgacccg cgccgccgtc atgtggtcct tcccttcctc gaggtgccgc
     1441 acgatgttct cccggacgac gatcgcgtcg tcgatcagga ggccgatcgc gagggagagc
     1501 gccatgagcg tcatgacgtt cagcgtcatc ccgaggaagt acatgacgat gaaggaggag
     1561 atgaccgaga tcggaagcgt cagccccgtg atcacggtcg agcgccagga gttgaggaag
     1621 aggaagacga tgaggacggt caggatcgcg ccgatgatca gggtcgtcgt gacgtcgtgg
     1681 accgactcct tgatcatcac ggagccgtcc ctcacgagct ggatctcgac ggacgggggg
     1741 agctcgcccc ggagcttctc gatctccttc gtcacggcct cgacgacgcc gaccgtgttc
     1801 gccttgctct gcttgaagac gtcgatcgcg acggccggga cgc
//
LOCUS       DAOTEZ010000630         1833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_57118_length_1833_cov_4.281215, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000630 DAOTEZ010000000
VERSION     DAOTEZ010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57118_length_1833_cov_4.281215"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="P5164_20180"
     CDS             complement(<1..1000)
                     /locus_tag="P5164_20180"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRY46260.1"
                     /translation="MARTIPALLLLLLGALRAPAAAAATPPAPGELARDPRVAQTLSL
                     LTAWFDAEQAHRRLPGLSLAVVRDQELLYARGFGHAHVETKSAATPQTMYSICSISKL
                     FTSVAVMQQRDAGRLRLDDLVSKHLPWFSIGGMPEGAPPVTVFGLLTHSSGLPREVDV
                     PYWTGPEYPFPPAGKVRAMVGAQEMLYRPETVFQYSNLGLTLAGEVAGAAAGTPWAEL
                     VTGGILDPLGMKATEVEHLDSWRGNRLATGYTAPRRDGTREKVPAYEVRGIAPAAGMT
                     STVEDLGRFASWQLRLLGSGKTELLDADTLREMQRVQFMDPGWKTSWGLGFQVWRSG"
     gene            complement(1064..1429)
                     /locus_tag="P5164_20185"
     CDS             complement(1064..1429)
                     /locus_tag="P5164_20185"
                     /inference="COORDINATES: protein motif:HMM:NF014687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HRY46261.1"
                     /translation="MGDPRVFFAAERTLLAWIRTGLTVMAFGFLVARFGVFLRIAAAQ
                     LGAEAHTPRFAPFSHAIGVCLVLAGTFSILVAAREHRAFLRTLPEDDRPAGRDARLHE
                     RLATFLGLLGVLLTVYLVL"
     gene            1597..>1833
                     /locus_tag="P5164_20190"
     CDS             1597..>1833
                     /locus_tag="P5164_20190"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRY46262.1"
                     /translation="MSGRLMELVSRAHLRWKRRVARDLAPYGVHPKQLFVLRKLVEAG
                     SLVPSEIAELVFADRPTTTSMLATMERSGWITRRR"
BASE COUNT          266 a          651 c          689 g          227 t
ORIGIN      
        1 cgccgctccg ccagacctgg aagccgagcc cccagctcgt cttccaaccc gggtccatga
       61 actggacccg ctgcatctcg cgcagcgtgt cggcgtcgag gagctcggtc ttcccgctcc
      121 cgaggaggcg gagctgccag gaggcgaaac ggccgaggtc ctccacggtg gaggtcatcc
      181 cggccgcggg agcgatgccg cgcacttcgt acgccggcac cttctcgcgc gtcccgtccc
      241 gccgcggggc ggtgtagccc gtcgcgagcc ggttcccgcg ccagctgtcg aggtgctcca
      301 cctccgtcgc cttcatcccg agcgggtcga ggatcccgcc cgtcacgagc tcggcccacg
      361 gcgtcccggc ggcggcgccg gcgacctcgc ccgcgagcgt gagcccgagg ttcgagtact
      421 ggaagaccgt ctcggggcgg tagagcatct cctgcgcgcc gaccatcgcg cggaccttcc
      481 ccgcgggcgg gaacggatac tccggcccgg tccagtacgg gacgtcgacc tcgcgcggga
      541 gcccggaaga gtgcgtgagg aggccgaaga cggtcaccgg cggcgctccc tcgggcatcc
      601 ctccgatcga gaaccagggc aggtgcttcg acacgaggtc gtcgagccgg agccgcccgg
      661 cgtcccgctg ctgcatcacc gcgacgctcg tgaagagctt cgagatcgag cagatcgagt
      721 acatcgtctg cggcgtcgcg gcgctcttcg tctcgacgtg ggcgtggccg aagccacggg
      781 cgtagaggag ctcctggtcg cggacgaccg cgagcgagag gccgggcagc cgccggtggg
      841 cctgctcggc gtcgaaccag gccgtcagga gcgagagcgt ctgcgcgacg cgcggatcgc
      901 gggccagctc gccgggcgcg ggcggcgtcg ccgcggcggc ggccggggcg cggagcgctc
      961 cgaggagaag aaggagcagg gccgggatcg ttctcgccat cgcgtcacct cccgccgggg
     1021 agacgacgat cggccggcgg gcgccggccg tcaagtcggc ggcctacagg acgaggtaga
     1081 cggtcaggag gacgccgagc aggccgagga acgtcgcaag ccgctcgtgg aggcgggcgt
     1141 cgcggccggc cgggcggtcg tcctccggga gcgtccggag gaaggcgcgg tgctcgcgcg
     1201 ccgccacgag gatcgagaag gtcccggcca ggacgagaca gaccccgatc gcgtgagaga
     1261 agggtgcgaa gcggggcgta tgcgcctcgg ccccgagctg ggccgcggcg atccggagga
     1321 agaccccgaa ccgggcgacg aggaacccga acgccatgac ggtcaggccg gtccggatcc
     1381 acgcgagaag cgtccgctcg gcggcgaaga agacgcgggg atcgcccatg cgcgaagagt
     1441 acacgggccg ctcgacggag cgcgccgcgg cccggcccgc ggcggtgtcc cgccgcagga
     1501 agcggctttc cccgcctggc cgttcgcccg gagcgatggt ccctggggcg aggtccgcgc
     1561 ttgcccccgt ccggatcgat agttagactt ctaatcgtgt ctggccggct gatggagctc
     1621 gtctcccgcg cccacctccg gtggaagcgg cgcgtcgcgc gagacctcgc accgtacggc
     1681 gtccatccga agcagctctt cgtcctccgg aagctcgtcg aggcggggag cctcgtgccg
     1741 agcgagatcg cggagctcgt cttcgccgac cgccccacca cgacctccat gctcgcgacg
     1801 atggagcggt ccggctggat cacgcgccgc cgg
//
LOCUS       DAOTEZ010000631         1832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_57170_length_1832_cov_4.453011, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000631 DAOTEZ010000000
VERSION     DAOTEZ010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57170_length_1832_cov_4.453011"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="P5164_20195"
     CDS             complement(<1..200)
                     /locus_tag="P5164_20195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRY46263.1"
                     /translation="MTVFIALLRKDPASDFGVDFPDFPGCVTAGSTVDEAVAMAREAL
                     GGHIVLRARPPGHTIVSRSERL"
     gene            complement(239..1123)
                     /locus_tag="P5164_20200"
     CDS             complement(239..1123)
                     /locus_tag="P5164_20200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46264.1"
                     /translation="MSVRRALSSLFVLVLVVAGGIYVRASLRRASAPPLPAREPSLDD
                     APARLYGLVEPSGREAFVGPLQSRRVLEVLVAEGAAVKAGAVLCRLDDEIERQALRVA
                     ELRVEEARRRRDLTEDDLGRKRRLSAEQVIPEFDLSRVALQARLDDQQIETARAEVEL
                     RRVELEKLTLRSPLTGIVYRMDVRAGEQLTPQDYGRIVVGLPGKQVRIFVETFWLGRL
                     KTGERLRVREAESGREVGTGTIRTISPYVGTRDFRTEDRLERIDTKFAQAVLELEAPT
                     DTPIGMQVVCEREALATR"
     gene            complement(1120..>1832)
                     /locus_tag="P5164_20205"
     CDS             complement(1120..>1832)
                     /locus_tag="P5164_20205"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY46265.1"
                     /translation="TLTLEAIARDFSDGRQMRRVLFPTDLVFTPGELTVLAGPSGSGK
                     TTLLSIMGLVLRPSEGRMRLDGQDVTALPDEAAAALRLSRYGFVFQQPMLIEGLSVLE
                     NVRLAFSVQGARTPAGFRKQAEELLASLGLAGTGHMQPRLLSGGMKQRASIARALIKE
                     PSVLLCDEPTSALDAESGQTVLEILKRIALEGERVVVVVSHDARVFPYADRLVKLENG
                     RVVSDTREGSPAVQETVA"
BASE COUNT          275 a          620 c          686 g          251 t
ORIGIN      
        1 ttcagccgct cgctgcgtga cacaatcgtg tgtccagggg gccgggcgcg taacacgatg
       61 tgacccccga gagcctcccg ggccatcgcg acggcctcgt cgacggtgct cccggcggtc
      121 acgcagcccg ggaaatccgg aaagtcgacg ccgaagtcgg aggcggggtc cttgcggagg
      181 agagcgatga agacggtcat gacagcttga ctccagactg tctgcggcgc acgcgccgtc
      241 accgcgtcgc gagtgcctct ctctcgcaga cgacctgcat cccgatagga gtgtccgtgg
      301 gcgcctcgag ctcgaggacc gcctgcgcga acttcgtgtc gatccgctcg aggcggtcct
      361 cggtccggaa gtcgcgcgtc ccgacgtagg gggagatggt ccggatcgtc cccgtgccga
      421 cctcgcggcc ggattcggcc tcccgcaccc gcagacgctc gccggtcttc aggcgcccca
      481 gccagaaggt ctcgacgaag atccggacct gcttgccggg gaggccgacg acgatgcggc
      541 cgtaatcctg gggtgtgagc tgctcgccgg cccgtacgtc catccggtag acgattccgg
      601 tcagcgggga gcggagcgtc agcttctcga gctcgacgcg ccggagctcc acctcggcgc
      661 gcgccgtctc gatctgctgg tcgtcgagcc gggcctgcag cgcgacgcgc gacaggtcga
      721 actccgggat gacctgctcg gcggagagcc ggcgcttcct cccgaggtcg tcctccgtca
      781 gatcgcgccg ccgccgggcc tcttccacgc gcagctcggc cacgcgcagg gcctgccgct
      841 cgatctcgtc gtcgagccgg cagaggacgg ccccggcctt cacggcggct ccctcggcga
      901 caaggacctc gaggacgcgc cgtgactgga gcggtccgac gaacgcctct ctcccggaag
      961 gctcgacgag gccgtagagg cgggcggggg cgtcgtcgag cgagggctcc cgcgcgggca
     1021 aggggggcgc cgaggcccgt cggagcgacg cgcggacgta gatccccccg gccaccacga
     1081 ggacgaggac gaaaagagag gagagggccc tgcggacgct catgcgacgg tctcctggac
     1141 ggccggcgac ccctcccggg tgtcggagac gacccggccg ttctcgagct tgacgaggcg
     1201 atcggcgtac gggaagacgc gggcgtcgtg cgagacgacg acgacgacgc gctctccctc
     1261 gagcgcgatc cgtttcagga tctcgaggac ggtctgcccg ctctcggcgt cgagggccga
     1321 ggtcggctcg tcgcagagga ggacggaggg ctccttgatg agcgcgcggg cgatggatgc
     1381 gcgctgcttc atgccgcccg agaggaggcg cggctgcatg tggccggttc cggcgagccc
     1441 gagggaggcg agaagctctt cagcctgctt gcggaagccc gccggcgtgc gcgctccctg
     1501 gaccgagaag gcgagccgga cgttctccag gacgctcagc ccctcgatga gcatcggctg
     1561 ctggaagacg aaaccgtagc gcgacaggcg gagggcggcg gccgcctcgt cggggagcgc
     1621 ggtgacgtcc tgcccgtcga gccgcatccg cccctccgag gggcgaagga cgaggcccat
     1681 gatggagagg agcgtggtct tgccgctgcc ggaggggccg gcgaggacgg tcagctctcc
     1741 gggcgtgaag acgaggtcgg tgggaaagag gacccggcgc atctgccggc cgtcggagaa
     1801 gtcgcgcgcg atcgcctcga gcgtcagcgt cg
//
LOCUS       DAOTEZ010000632         1823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_57644_length_1823_cov_5.067873, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000632 DAOTEZ010000000
VERSION     DAOTEZ010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57644_length_1823_cov_5.067873"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="P5164_20210"
     CDS             complement(<1..596)
                     /locus_tag="P5164_20210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRY46266.1"
                     /translation="MSRPRTPVRILILLLGMVAAGSLAYALSNVRKGYEPRQPILFRH
                     SRMAGPAVMQTNEKGEAVNVGGYDIPCLTCHTMPYKGRHSTVPSTAVCMNCHTAVGLN
                     REWVLKMKDYWDRGEPIPWVKVHDLPDFVYFDHSAHLNAKDEKGNPKLPLQDADGRPW
                     QVCANCHGRVEETDIVSVTNAFNMQWCLDCHRKPEMKAS"
     gene            complement(593..835)
                     /gene="ccoS"
                     /locus_tag="P5164_20215"
     CDS             complement(593..835)
                     /gene="ccoS"
                     /locus_tag="P5164_20215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HRY46267.1"
                     /translation="METIFVLLPLAVLIAGIALALFVWAARSGQFDDLETPAVRMLFD
                     DEPAPAAPRPALGAPADPPTPNPPETTSDAPTRRTE"
     gene            complement(836..>1823)
                     /locus_tag="P5164_20220"
     CDS             complement(836..>1823)
                     /locus_tag="P5164_20220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRY46268.1"
                     /translation="GTVLLDKTGTLTEGKASFARYLGDETALELASTLEAESSHAVAH
                     ALRTSLGRRVTVVREVARVREVAGRGISGVVDGRHVAVGNRAHVAAFTSSVPEALDAH
                     AEALVADGFSPLWVAVDGHVAGVGGIGDPLRAHARATVDALRARGVLVRVLSGDDPRV
                     VASVAARLGIAPEHALGGRTPEEKRDLVASLVAARESEGRDAPAVVMVGDGVNDAAAL
                     ALADVGVAVLGGKGASLVAADVVLTREGVAPLLELLRGSRRVFGVIRRNLVFSLLYNV
                     AGAALALAGLVGPLLAAVLMPASSLSVVLSSALSRPFPAPARRTPRGRSQEA"
BASE COUNT          256 a          596 c          734 g          237 t
ORIGIN      
        1 gaggccttca tctcgggctt gcggtggcag tcgaggcacc actgcatgtt gaaggcgttc
       61 gtgacggaga cgatgtcggt ctcctcgacg cggccgtggc agttcgcgca gacctgccag
      121 gggcgcccgt ccgcgtcttg cagcggcagc ttcgggttcc ccttctcgtc cttcgcgttc
      181 aggtgggccg agtggtcgaa gtagacgaag tcggggaggt cgtggacttt cacccagggg
      241 atcggctcgc cccggtccca gtagtccttc atcttgagga cccactcgcg gttcaggccg
      301 accgccgtgt ggcagttcat gcagacggcg gtggacggga cggtggagtg gcgccccttg
      361 tagggcatcg tgtggcaggt gaggcagggg atgtcgtagc cccccacgtt caccgcctcg
      421 cccttctcgt tcgtctgcat cacggcgggc ccggccatcc gcgagtgccg gaagaggatc
      481 ggctgtcgcg gctcgtaccc cttgcgcacg ttgctcagcg cgtacgccag ggagcccgcc
      541 gcgaccatcc cgaggaggag gatcaggatg cggacgggcg ttctcggtcg gctcattcgg
      601 tcctccttgt cggcgcgtcg gaggtggtct ccggaggatt cggggtcggg ggatcggccg
      661 gcgcgcccag cgcggggcgc ggggcagcgg gtgccggctc gtcgtcgaag agcatccgga
      721 cggcgggcgt ctcgaggtcg tcgaactgcc ccgagcgcgc ggcccagacg aagagcgcga
      781 gggcgatccc cgcgatgagg acggcgagcg ggaggaggac gaagatcgtc tccacctagg
      841 cctcctgcga acgtccgcgc ggggtccggc gggccggcgc ggggaagggc cgcgagagag
      901 cggacgagag gacgacgctc agcgaggagg ccggcatcag gacggccgcg aggagcggcc
      961 cgacgaggcc ggcgagcgcc agggccgcgc ccgccacgtt gtagaggagg gaaaatacga
     1021 ggttccggcg gatgacgccg aagacgcgcc gggacccgcg gaggagctcg aggagcgggg
     1081 cgaccccttc gcgcgtcagg acgacgtcgg cggcgacgag cgaggccccc ttcccgccga
     1141 ggacggcgac gccgacgtcg gccagagcaa gggcggcggc gtcgttgacg ccgtcgccga
     1201 ccatgacgac ggcgggggcg tcgcgacctt cgctttcgcg cgccgccacg agcgaggcga
     1261 cgaggtcgcg cttctcttcc ggcgtccgcc cgccgagggc gtgctccgga gcgattccga
     1321 ggcgggcggc gacggaggcg acgacgcgcg ggtcgtcgcc ggaaaggacc ctcacgagga
     1381 cgccccgggc gcggagcgcg tcgaccgtgg cgcgcgcgtg ggcgcggagc gggtcgccga
     1441 tgccgccgac gccggcgacg tggccgtcga cggcgaccca gagcgggctg aatccgtcgg
     1501 cgacgagcgc ctcggcgtgc gcgtcgagag cctccgggac ggaggaggtg aacgccgcga
     1561 catgcgcgcg gttcccgacg gcgacgtggc gcccgtcgac gacgccggag atgccgcgtc
     1621 ccgcgacctc gcggacccgg gccacctcgc ggacgacggt cacgcggcgg ccgagggagg
     1681 tgcggagggc gtgggcgacg gcgtgcgagg actccgcctc gagggtcgag gcgagctcca
     1741 gggctgtctc gtcgccgagg taacgggcga acgaagcctt cccctcggtg agcgtcccgg
     1801 tcttgtcgag gaggacggtg ccg
//
LOCUS       DAOTEZ010000633         1817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_57967_length_1817_cov_5.871169, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000633 DAOTEZ010000000
VERSION     DAOTEZ010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57967_length_1817_cov_5.871169"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1817
                     /locus_tag="P5164_20225"
     CDS             <1..>1817
                     /locus_tag="P5164_20225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46269.1"
                     /translation="RAGDVPGALAVERAAIAAVRAAGGDEGDALAARQTARAARRFLR
                     LGRANAAWDLAAPGGDPARCGEVPLEHAERTEIALRAGAFPKLFGRWAADDAFLEESA
                     WPFSRNALPEQREAAEKEILARVFLDGGRVDERALTRLHAWAESAGIRRLNEALARRL
                     LAAVPPAKAFWGADPPADFVASLDPIERYTTKDGASRLRLVRTDFHAEWIRHLVARDD
                     LASLRPALVPLVDELHAKVTGPAPVTKALPWAGAFPVEAFARLAALPENASWKRAVET
                     WLSSYGVHQRFLAATGRSFGEKALLPLLTDGARRAWFVRGGPPTSPGPEAAGARTARN
                     AAVDRAADALWQLVSGEGGAGASADVARLRGPRTVGELLGKDPRFVWSELAPTPGDRG
                     DDLVTGSGVDAGRVPARIWGTTPAAPWYVLESLARLRERGGDAPLVPLESASRGGEAL
                     RASVAVRTAQALGDLPLALSLDEAWFTDLSRAERLDRRLRLLVAAGGAAGKSRADGLL
                     REEVRSRQAKASEPLFRAWERSAAALGLTPPLASLDPSVPVESGLLALLCDREGPAAV
                     ASLKPADRAEYRGSLGGRWSARVGSLSPDRLDHFLREVW"
BASE COUNT          192 a          738 c          663 g          224 t
ORIGIN      
        1 ccgggcgggc gacgtgccgg gggcgctcgc cgtcgagcgg gccgcgatcg ccgccgtccg
       61 tgccgcgggc ggcgacgagg gggacgccct cgccgcccgg cagacggccc gcgcggcccg
      121 ccgcttcctg cggctcggcc gcgcgaacgc ggcctgggat ctcgccgcgc ccggcggcga
      181 cccggcgcgc tgcggcgagg tgccgctcga gcacgccgag aggaccgaga tcgccctccg
      241 cgccggagcg ttcccgaagc tcttcgggcg ctgggcggcg gacgacgcgt tcctcgagga
      301 gagcgcgtgg cccttctccc gcaacgccct ccccgagcag agggaggcgg ccgagaagga
      361 gatcctcgcc cgcgtcttcc tcgacggagg ccgcgtcgac gagcgcgctc tcacgcgcct
      421 ccacgcgtgg gccgagtcgg cggggatccg ccgcctgaac gaggcgctcg cgcggaggct
      481 gctcgccgcg gtcccgccgg cgaaggcgtt ctggggagcg gacccgccgg ccgacttcgt
      541 cgcgagcctc gacccgatcg agcgatacac gacgaaggac ggcgcgtccc gcctccgcct
      601 cgtccgcacc gacttccacg ccgagtggat ccgccacctc gtcgcgcgcg acgacctcgc
      661 gtcgctccgc ccggcgctcg tgccgctcgt cgacgagctg cacgcgaagg tgaccggccc
      721 ggcccccgtc acgaaggcgc ttccctgggc cggcgcgttc cccgtcgagg cgttcgcgcg
      781 cctcgccgcg cttcccgaga acgcctcgtg gaagagggcg gtcgagacct ggctttcctc
      841 gtacggcgtg caccagcgct tcctcgcggc cacgggccgc tccttcggcg agaaggcgct
      901 cctgccgctc ctgacggacg gcgcgcgccg agcctggttc gtccgcggcg ggccgccgac
      961 cagccccggc ccggaggcag cgggcgcgcg gaccgcccgc aacgccgccg tcgaccgggc
     1021 tgccgacgcc ctctggcagc tcgtctcggg agaagggggc gccggcgcga gcgccgacgt
     1081 cgcgagactc cgcggccccc gcaccgtcgg cgagctcctc gggaaggacc cgcgcttcgt
     1141 ctggagcgag ctggccccga cgccgggcga ccgcggcgac gacctcgtca cgggttccgg
     1201 cgtcgacgcg ggccgcgtcc cggcgcggat ctggggcacg acccccgcgg caccgtggta
     1261 cgtcctcgag agcctcgcgc gccttcgcga gcgaggcggg gacgctcccc tcgtcccgct
     1321 cgaatccgct tctcgcggag gcgaggccct tcgcgcctcc gtcgcggtga ggaccgccca
     1381 ggccctcggc gaccttccgc tcgcgctctc tctcgacgag gcgtggttca ccgacctctc
     1441 gcgggccgag cggctcgacc gccggcttcg gctcctcgtc gccgcgggag gtgccgccgg
     1501 gaagtcccgg gccgacggcc tcctgcgcga ggaagtccgc tcccggcagg cgaaggcctc
     1561 cgagcccctc ttccgcgcct gggagagaag cgccgccgcg ctcggcctga cgcccccgct
     1621 cgcgagcctc gacccgtccg ttccggtcga atccggcctc ctcgcgctcc tctgcgaccg
     1681 ggaagggccc gccgccgtcg cttcgctgaa gcccgcggac cgggcggagt accggggaag
     1741 cctcggcggc cgttggagcg cccgcgtcgg gtcgctctct cccgaccggc tcgaccactt
     1801 ccttcgcgag gtctggg
//
LOCUS       DAOTEZ010000634         1813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_58197_length_1813_cov_7.422071, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000634 DAOTEZ010000000
VERSION     DAOTEZ010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58197_length_1813_cov_7.422071"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="P5164_20230"
     CDS             <1..354
                     /locus_tag="P5164_20230"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRY46270.1"
                     /translation="LDEERCIKCTLCAIECPINIIYIDWHTEKTPEGKAEKKLDRFDL
                     DMKRCMFCGLCEEACPTNPRSIWLATKTYELGVYERDEALYLDKSKLTKWDGNVKPYP
                     GVDPDKAPANPAKEK"
     gene            356..910
                     /locus_tag="P5164_20235"
     CDS             356..910
                     /locus_tag="P5164_20235"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRY46271.1"
                     /translation="MEPGLFFFWLFSAVVVVGAVGVVVMRQPIHSALFLLLSFLGVAC
                     LYVQARAELVAAVQVLVYAGGVMVLFLYTIMLVNVKRQRTERYLHRQAVPALGFGFLF
                     LLVVGGVVVNEARALAGKAPAADPAALATTVIGSETVLGNTQAVGWVLYRDYLLPFEI
                     ASLFLLVAMIGAVVLGKRTMERFD"
     gene            911..1213
                     /gene="nuoK"
                     /locus_tag="P5164_20240"
     CDS             911..1213
                     /gene="nuoK"
                     /locus_tag="P5164_20240"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRY46272.1"
                     /translation="MITLAHFVGLSFVVFTIGVVGVLVRRNLLTVLMSIELILNAVNI
                     NLVAFSRQLGDLTGQVFSVFVIIVAAGEAAIGLAIVLSLYRLKSSVNLEDAAEMRG"
     gene            1218..>1813
                     /locus_tag="P5164_20245"
     CDS             1218..>1813
                     /locus_tag="P5164_20245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46273.1"
                     /translation="MSAETLLPWIPVFPLLGFLVNGLLYLVAHSKLGGKDAPLGAHGH
                     GGGHGDSHAAHAADPDDHGHGGHGDIPFKAVHTWIGPVASGLSFVFALLAILSWWKET
                     GGHREVVATLWTWIPMGTNAGWFGAPDFTVDVAFRLDPLSALMLGFVTFIGTLIHVYS
                     VGYMGHDEGFGKFFAYLNLFMFAMLTLVLGANLAILFVG"
BASE COUNT          260 a          652 c          569 g          332 t
ORIGIN      
        1 ctcgacgagg agcgctgcat caagtgcacg ctctgcgcga tcgagtgccc gatcaacatc
       61 atctacatcg actggcacac cgagaagacg cccgagggga aggccgagaa gaagctcgac
      121 cgtttcgacc tcgacatgaa gcggtgcatg ttctgcggcc tctgcgagga ggcctgcccg
      181 acgaacccgc gctccatctg gctcgccacg aagacctacg agctcggcgt ctacgagcgc
      241 gacgaggcgc tctacctcga caagtcgaag ctgacgaagt gggacgggaa cgtgaagccg
      301 taccccggcg tcgaccccga caaggcgccg gcgaacccgg ccaaggagaa gtgacgtgga
      361 accgggcctc ttcttcttct ggctcttctc cgcggtcgtc gtcgtcggcg ccgtcggcgt
      421 cgtcgtcatg cgccagccga tccactcggc gctcttcctg ctcttgtcgt tcctcggggt
      481 cgcctgcctc tacgtccagg cgcgggccga gctcgtcgcc gccgtccagg tcctcgtcta
      541 cgcgggcggc gtcatggtcc tcttcctcta cacgatcatg ctcgtgaacg tgaagcggca
      601 gcggacggag cgctacctcc accggcaggc cgtcccggcc ctcgggttcg gcttcctctt
      661 cctcctcgtc gtcggcgggg tcgtcgtgaa cgaggcgcgg gcgctcgcgg ggaaggcccc
      721 ggcggccgac ccggccgccc tcgccacgac ggtcatcgga agcgagacgg tcctcgggaa
      781 cacgcaggcg gtcggctggg tcctctaccg cgactacctc ctgccgttcg agatcgcctc
      841 cctcttcctc ctcgtcgcga tgatcggcgc cgtcgtcctc ggcaagcgga cgatggagag
      901 gttcgactag atgatcaccc tggcccactt cgtcggactc tcgttcgtcg tcttcacgat
      961 cggcgtcgtc ggcgtcctcg tgaggaggaa cctcctgacc gtcctgatgt cgatcgagct
     1021 gatcctgaac gccgtgaaca tcaacctcgt cgccttctcg cgccagctcg gcgacctgac
     1081 ggggcaggtc ttctcggtct tcgtcatcat cgtggcggcg ggggaggcgg cgatcggcct
     1141 cgcgatcgtc ctctcgctct accggctgaa gtcgtcggtg aacctcgagg acgccgcgga
     1201 gatgaggggc tgagccgatg agcgccgaaa cgctcctccc ctggatcccg gtcttcccgc
     1261 tcctcggatt cctcgtcaac gggctcctct acctcgtcgc ccactcgaag ctcgggggaa
     1321 aagacgcccc gctcggcgcc cacgggcacg gcggcggcca cggggactcc cacgccgcgc
     1381 acgcggccga tcccgacgac cacggtcacg gcgggcacgg cgacattccc ttcaaggcgg
     1441 tccacacgtg gatcggcccc gtcgcctcgg gcctctcgtt cgtcttcgcc ctcctcgcga
     1501 tcctctcctg gtggaaggag acgggcggcc accgcgaggt cgtcgcgacc ctctggacct
     1561 ggattccgat ggggacgaac gcgggctggt tcggcgcccc cgacttcacg gtcgacgtcg
     1621 ccttccgcct cgacccgctc tccgccctga tgctcgggtt cgtgacgttc atcgggacgc
     1681 tcatccacgt ctactccgtc ggctacatgg ggcacgacga ggggttcggg aagttcttcg
     1741 cctacctgaa cctcttcatg ttcgcgatgc tgacgctcgt cctcggggcg aacctggcga
     1801 tcctcttcgt cgg
//
LOCUS       DAOTEZ010000635         1806 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_58600_length_1806_cov_5.912621, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000635 DAOTEZ010000000
VERSION     DAOTEZ010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58600_length_1806_cov_5.912621"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..126
                     /locus_tag="P5164_20250"
     CDS             <1..126
                     /locus_tag="P5164_20250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009369157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HRY46274.1"
                     /translation="PLCRPECKGLCPRCGADRNIAPCDCREEGDERLAKLKSLLT"
     gene            192..377
                     /gene="rpmF"
                     /locus_tag="P5164_20255"
     CDS             192..377
                     /gene="rpmF"
                     /locus_tag="P5164_20255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRY46275.1"
                     /translation="MPNPKHRHSKTRRDMRRAHDFLQEPGLSKCASCGATKTPHRVCP
                     ECGHYRGREVSAGAQKS"
     gene            404..1417
                     /gene="plsX"
                     /locus_tag="P5164_20260"
     CDS             404..1417
                     /gene="plsX"
                     /locus_tag="P5164_20260"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRY46276.1"
                     /translation="MTLAVDAMGGDNAPRATVEGAVAAAVDFGLDLLLVGRRRELEPI
                     LSATSYRGSRIDLVEAEEVVGFDEPSTTALRRKPRASIRIGAELVRDGRAEGFFTAGH
                     TGAAMVTARSVLGVVDGIDRPALGAVLPGLGRRRLLLDVGANVSCRVEHYREFALMGH
                     FYAQEVLGISRPKIGLLSVGEEEEKGTGTTREVFGLLRKSGLNFVGNCEGRDVYGGNV
                     DVDVIVTDGFTGNVVLKTSESLARLVEAALKQEITRSLQASMGFLLTRDAFRAFKKRL
                     DYTEYGGAPLLGVKGAVVIGHGSSNAHAVRNGIKALQEYARRRIPEKIRAKARELAPL
                     AAR"
     gene            1434..>1806
                     /locus_tag="P5164_20265"
     CDS             1434..>1806
                     /locus_tag="P5164_20265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HRY46277.1"
                     /translation="MFHAAIAGTGRAVPAKLLTNADLETLVETTDEWITTRTGIRERH
                     KAADGEVLSDFCVTAATQALESAGLAPKDLDAVLIATVTPDQPIPAVACLVQQKLGAK
                     KAAAWDQNAGCSGWLYGLHIAD"
BASE COUNT          292 a          648 c          644 g          222 t
ORIGIN      
        1 ccgctctgcc ggcccgagtg caaggggctc tgcccccgct gcggcgccga ccggaacatc
       61 gccccgtgcg actgccggga agaaggcgac gagaggctcg cgaagctcaa gtcgctcctg
      121 acctaggccg ccgaaggcga cgacgccccg aggcgccccc gacacgaaac gatcccgaga
      181 ggtgaactgc catgccgaat ccgaagcacc gtcacagcaa gacccgccgc gacatgcgga
      241 gggcgcacga cttcctccag gagccgggcc tctccaagtg cgccagctgc ggcgcgacca
      301 agacccccca ccgcgtctgc cccgagtgcg gccactaccg cggacgggaa gtctccgccg
      361 gcgcccagaa gagctgatcg cggcccttcc ggtcgcgtcc tgaatgaccc tcgccgtcga
      421 cgccatgggg ggagacaacg ccccccgtgc caccgttgaa ggagccgtcg cggccgccgt
      481 cgacttcggc ctcgacctcc tcctcgtggg gcgccggcgc gagctggagc cgatcctctc
      541 ggcgacgagc taccggggga gccggatcga cctcgtcgag gcggaggagg tcgtcggctt
      601 cgacgagccg tcgacgaccg ccctccggcg gaagccccgt gcctcgatcc gcatcggggc
      661 cgagctcgtc cgcgacggcc gggcggaggg cttcttcacg gccggccaca cgggcgcggc
      721 gatggtgacg gcccgctccg tcctcggcgt cgtcgacggg atcgaccgtc ccgcgctcgg
      781 cgcggtcctg ccggggctcg ggcggaggag gctcctcctc gacgtcgggg cgaacgtctc
      841 gtgccgcgtc gagcactacc gcgagttcgc gctgatgggc cacttctacg cccaggaggt
      901 cctcggcatc tcgcggccga agatcggcct cctctccgtc ggggaggagg aggagaaggg
      961 gaccggcacg acgcgggagg tcttcggcct cctgcggaag tcgggcctga acttcgtcgg
     1021 gaactgcgag gggcgcgacg tctacggcgg aaacgtcgac gtcgacgtca tcgtcaccga
     1081 cgggttcacg gggaacgtcg tcctcaagac gtccgagagc ctcgcgcggc tcgtcgaggc
     1141 ggcgctgaag caggagatca cgcggagcct ccaggcctcg atggggttcc tcctgacgag
     1201 ggacgcgttc cgggcgttca agaagcggct cgactacacc gagtacggcg gcgcgccgct
     1261 cctcggggtg aagggggccg tcgtcatcgg ccacggcagc tcgaacgctc acgccgtgag
     1321 gaacgggatc aaggccctcc aggagtacgc caggcgcaga atcccggaaa agatccgcgc
     1381 gaaggcccgc gagctggcgc ccctggccgc acgctgaaga gaggagacgg gagatgttcc
     1441 acgccgcgat cgccggcacg ggccgggccg tacccgcgaa gctcctgacg aacgccgacc
     1501 tcgagacgct cgtcgagacg accgacgagt ggatcacgac ccggaccggg atccgggagc
     1561 gccacaaggc ggccgacggc gaggtcctct cggacttctg cgtgaccgcg gcgacgcagg
     1621 cgctcgagag cgcgggcctc gcgccgaagg acctcgacgc cgtgctgatc gcgacggtga
     1681 ccccggacca gccgatcccg gccgtggcgt gcctcgttca gcagaagctc ggggccaaga
     1741 aggccgcggc gtgggaccag aacgccggct gctccggctg gctctacggc ctccacatcg
     1801 ccgacg
//
LOCUS       DAOTEZ010000636         1800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_58951_length_1800_cov_6.570201, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000636 DAOTEZ010000000
VERSION     DAOTEZ010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58951_length_1800_cov_6.570201"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1403
                     /locus_tag="P5164_20270"
     CDS             <1..1403
                     /locus_tag="P5164_20270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015233943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HRY46278.1"
                     /translation="SPPTPHGFKGGDLLGLAGKLDDLSALGVTALYLNPVFASAANHR
                     YHTWDYHQVDPILGGNEALRELLDAAHARGMKVVLDGVFNHVGRGFFAFHSVLENGAD
                     SPYLDWFHLDVERLQDGRPLGAYAEPKLLRRSKRPKPAFEELGYSAWWDCPALPKLDT
                     RTPAVREYLWDVAERWVRFGIDGWRLDVPSEIDDDEFWRELRRRVKRANPEAYLVGEI
                     WHDARRWLAGDQFDATMNYPLAKAALGFFVPDLETDALRSVGGYRDVVELDAAAFAAE
                     VERVLSLYAPAVRDAQLNLIGSHDTPRFLTLAGGELAALEMAFLFLFTMPGAPCIYYG
                     DEIGLEGGPDPDCRRAYPWEEGSWDHVLREQVRRLAALRRAHPALRRGSFRTILAEDA
                     IVSYVREEEGGDAVVVAFNASREERRVDLPVGALPARLLDAATGDDLVVTEGELRGID
                     LPGRSGRAFVTPPAAG"
BASE COUNT          236 a          686 c          614 g          264 t
ORIGIN      
        1 actccccgcc gacgccccac ggcttcaagg gaggcgacct cctcggcctc gccgggaagc
       61 tcgacgacct ctcggcgctc ggcgtcacgg cgctctacct caaccccgtc ttcgcctcgg
      121 cggcgaacca ccgctaccac acctgggact accaccaggt cgacccgatc ctcggcggga
      181 acgaggcgct tcgcgagctc ctcgacgcgg cccacgcgcg cggcatgaag gtcgtcctgg
      241 acggagtctt caaccacgtc gggcgcggct tcttcgcgtt ccacagcgtc ctcgagaacg
      301 gcgccgactc cccctacctc gactggttcc acctcgacgt cgagcgcctg caggacggcc
      361 ggcccctcgg cgcctacgcc gagccgaagc tcctgcgccg ctcgaaacgg ccgaagccgg
      421 cgttcgagga gctcggctac tcggcctggt gggactgccc ggccctgccg aagctcgaca
      481 cgcggacgcc cgccgtccgc gagtacctct gggacgtcgc ggagcgctgg gtccggttcg
      541 ggatcgacgg ctggcggctc gacgtcccgt cggagatcga cgacgacgag ttctggcgcg
      601 agctgcgccg ccgcgtgaag cgcgcgaacc ccgaggccta ccttgtcggg gagatctggc
      661 acgacgcccg ccgctggctc gcgggcgacc agttcgacgc gacgatgaac tacccgctcg
      721 cgaaggcggc tctcggcttc ttcgtcccgg acctcgagac ggacgctctc cggtcggtcg
      781 gaggctaccg ggacgtcgtc gagctcgacg ccgccgcgtt cgccgcggag gtggagcgag
      841 tgctcagcct ctacgccccg gccgtccggg acgcgcagct caacctcatc gggagccacg
      901 acacgccccg cttcctcacg ctcgcgggcg gggagctcgc tgcgctcgag atggcgttcc
      961 tcttcctctt cacgatgccc ggcgccccct gcatctatta cggcgacgag atcggcctcg
     1021 agggggggcc cgaccccgac tgccggcgcg cgtacccgtg ggaggagggc tcgtgggacc
     1081 acgtcctgcg cgagcaggtc cggcgcctcg ccgccctccg tcgcgcccac cccgcgctcc
     1141 gccgcggctc gttccgcacg atcctggccg aggacgcgat cgtctcctac gtccgcgagg
     1201 aggagggcgg cgacgcggtc gtcgtcgcct tcaacgcctc ccgcgaggag cggcgcgtcg
     1261 acctcccggt cggggcgctc ccggcgcgcc tcctcgacgc ggccacgggc gacgacctcg
     1321 tcgtgaccga aggggagctg cgcggaatcg acctcccggg ccgctcgggc cgcgcgttcg
     1381 tcacgcctcc ggccgccggc tgaggccggc ccttccggcg ggacgcgcct cggcgaggcg
     1441 atctgccccg gatggggtcc cttcgtgcgc gcagcctgat gcatttacat catcacgtga
     1501 ccccgccccg actttgcgac gtccacgcag cattcgcaca aaccacgctc gcttcgattg
     1561 ttgtcgaaac gagagggcgg gcgtgcggtc cgcgtttcgc cgctcaatcg ggcccgttct
     1621 gggcctgaat tgaagcgtct ggaggatggt gctgcgctgc aacgggacgg aacatgcccg
     1681 ccggatcagg cagcaggagc cgtgccgcca gggggccgcg tgtcgggaga gatccaggga
     1741 gcgatgcacc tcggggaggc gcctgggcgt cttcgcggct tcgcgcccgt ccccgccggc
//
LOCUS       DAOTEZ010000637         1798 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_59087_length_1798_cov_5.291452, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000637 DAOTEZ010000000
VERSION     DAOTEZ010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59087_length_1798_cov_5.291452"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..>1798)
                     /locus_tag="P5164_20275"
     CDS             complement(113..>1798)
                     /locus_tag="P5164_20275"
                     /inference="COORDINATES: protein motif:HMM:NF015754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCCL domain-containing protein"
                     /protein_id="HRY46279.1"
                     /translation="LDVFGEEEAWVRRVLSEPGVPALLGRLLRFQGPFARRQLVLRPG
                     ALNVTFHLSTAFMDWIPTTAQVKDWADALVALAEIVERVPPPEVPIAPSRLEQQVDSV
                     RRMGFAVNPGALALGTMLVTSLVIAAIAGILVVAQKSCRPAKGPGSHEEGTRVVSNVV
                     QVVRSLQVVEMKPLDVLGGLGVLDREDADALHARAGHPHDARIVIRKDPSNGAGREVE
                     WLFPTPPAIRLAYLEEQLGRLEYSADAGDPKTVVGVAAPKAVDGAKWPVRVRVAFVGE
                     AGGPVSRITVSREAPTGVAPAAPPADAAAVAGSPSEAATAPDPALAPASAAATRAALL
                     SPCPTSFADAGPMEVGESRSCVCPHLGASGTVRGTGRYAPSSWICDAARHAGVLQKPG
                     DAVTFTRQPDCPRLWGSRANEKLSLNFGAPTATFSFGSEPPPCPPPPSAAAGPGPCPS
                     SGKNGLDAMPEGSSFDCTCTYNGIVHGRLQKGDRGVAWGTNVYATFSNVCDAAQHAGA
                     VDFGGSTVTVFLGGTCERFFGTKRFGIGSDGWSRPARAMSFQSPFPVCPTGPW"
BASE COUNT          241 a          659 c          680 g          218 t
ORIGIN      
        1 gtccccgcgg tccagtggct catggcgctg ccggccgtcg tcgcggcgct cgtcctgcgg
       61 ccgaaggagg cttccgcgcc tcgctgagga accgcccggg cggggcgggc cgtcaccacg
      121 gccccgtcgg gcagacgggg aaggggctct ggaacgacat cgcgcgcgcg ggcctgctcc
      181 agccgtcgga gccgatcccg aaccgcttcg tgccgaagaa gcgctcgcac gtcccgccga
      241 ggaagacggt cacggtgctc cccccgaagt cgaccgcccc cgcgtgctgg gccgcgtcgc
      301 agacgttcga gaaggtggcg tagacgttcg ttccccacgc cacgccccgg tcccccttct
      361 ggagccgtcc gtggacgatc ccgttgtacg tgcaggtgca gtcgaagctc gatccctccg
      421 gcatcgcgtc gaggccattc ttcccgctgg acgggcaggg ccccgggccc gcggcggcgc
      481 tcggcggcgg cgggcacgga ggcggctcgg acccgaagga gaacgtggcc gtcggcgccc
      541 cgaagttgag cgagagcttc tcgttcgccc tcgatcccca gaggcgcggg cagtccggct
      601 gccgggtgaa ggtgacggcg tcgccgggct tctggaggac gccggcgtgg cgcgccgcgt
      661 cgcagatcca ggaggagggc gcgtagcgcc ccgtcccgcg gacggtcccg gaggcgccga
      721 ggtgaggaca gacgcacgag cgcgactcgc cgacctccat cggcccggcg tccgcgaacg
      781 acgtggggca gggggagagg agcgcggctc gggtcgcggc tgcggacgcg ggagcgagag
      841 ccgggtccgg agcggtggcc gcctccgacg gcgatccggc gacggccgcg gcgtcggcgg
      901 ggggagcggc cggggcgacg cccgtcggcg cctcgcgcga gaccgtgatc cgagagaccg
      961 gcccgcccgc ctcgccgacg aacgccaccc ggacgcggac cggccacttc gcgccgtcga
     1021 ccgccttcgg cgccgcgacc ccgacgaccg tcttcgggtc gccggcgtcg gcggagtact
     1081 cgagccttcc gagctgctcc tcgagatacg ccaggcggat cgccggaggc gtcgggaaga
     1141 gccactccac ctcccgcccg gcgccgttcg acgggtcctt ccggatgacg atccgggcgt
     1201 cgtgcggatg gcccgccctc gcgtggaggg cgtccgcgtc ctcccggtcg aggacgccga
     1261 gcccgccgag gacgtccagg ggcttcatct cgacgacctg gaggctccgg acgacctgca
     1321 ccacgttcga gacgacccgg gtgccctcct cgtggctccc gggccccttc gctggccggc
     1381 acgacttctg cgcgacgacg aggatccccg cgatcgcggc gattacgagg gaggtgacga
     1441 gcatcgtgcc gagggcgagc gcgccgggat tgacggcgaa gcccatgcgc cgcacggagt
     1501 cgacctgctg ctcgaggcgg ctcggggcga tcgggacttc cggcgggggg acccgctcga
     1561 cgatctccgc cagcgccacg agcgcgtcgg cccagtcctt cacctgcgcc gtcgtgggga
     1621 tccagtccat gaaggcagtc gagaggtgga acgtgacgtt gagcgccccg ggccggagga
     1681 ccagctgccg gcgagcgaac ggcccctgga acctcaggag ccttccgagg agcgccggga
     1741 cccccggctc ggaaaggacg cggcgcaccc acgcctcctc ctcgccgaag acgtcgag
//
LOCUS       DAOTEZ010000638         1792 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_59439_length_1792_cov_4.523892, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000638 DAOTEZ010000000
VERSION     DAOTEZ010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59439_length_1792_cov_4.523892"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="P5164_20280"
     CDS             <1..226
                     /locus_tag="P5164_20280"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRY46280.1"
                     /translation="LGSVVALGGLLLGVTLRAGPPPVPAAGGLIPAKDEIPILGRLLF
                     SDYLLAFEALSVLLLLAAVGALVLSKRRFD"
     gene            261..566
                     /gene="nuoK"
                     /locus_tag="P5164_20285"
     CDS             261..566
                     /gene="nuoK"
                     /locus_tag="P5164_20285"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRY46281.1"
                     /translation="MTVPLNAFLAVGTILFVIGLAGVLLKRNALSMFLSIELMMNAVN
                     LLFLAYARMRGDASGQMVVFFVIAVAAAEAAVGLAIFVALFRAKRTIDVDEIASLKW"
     gene            580..>1792
                     /locus_tag="P5164_20290"
     CDS             580..>1792
                     /locus_tag="P5164_20290"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRY46282.1"
                     /translation="MNLPLWLIPALPLAGFLLNGSVALFSGWRRARKATAAWREAHPG
                     DHGHDEHGGHAADDHGHDDHGHHGPFLPYGERLFYGVVGVLSVGLATLVAFANVVPYV
                     AASLATEGGVGPVVQSVWKWMAAGHVSIDMAFRLDPLSAMMVSFVTFVGLLIHVFSVG
                     YMQDEEGYGRYFAYLNLFMFAMLTLILADSLVVLFIGWEGVGLCSYLLIGYYYDKDFA
                     HAAGKKAFVTNRIGDFGMVLGIFGILALFGTADFSFVDMAKAGAVNGTASITVYAVCL
                     LLFLGAVGKSAQVPLYVWLPDAMAGPTPVSALIHAATMVTAGVYLVARCSAVFVLAPD
                     ALTLVAWVGAGTAVFAATIGLAQNDIKKVLAYSTVSQLGYMFLACGVGAFGVGMFHVF
                     THAFFKACLFLG"
BASE COUNT          214 a          662 c          579 g          337 t
ORIGIN      
        1 cctcggctcg gtcgtcgcgc tcggtgggct cctcctcggc gtcacgctcc gggcggggcc
       61 gccgcccgtg ccggccgccg gcggcctcat cccggcgaag gacgagatcc cgatcctcgg
      121 gaggctcctc ttctccgact acctgctcgc gttcgaggcg ctctcggtgc tgctcctgct
      181 ggcggccgtc ggcgcgctcg tcctctccaa gcggaggttc gactgagatg gagggcgccg
      241 cgatgaacgc cgtcgtcccg atgaccgtcc ccctgaacgc cttcctggcc gtcgggacga
      301 tcctcttcgt catcgggctc gccggggtgc tcctgaagag gaacgccctc tcgatgttcc
      361 tctcgatcga gctgatgatg aacgccgtga acctcctctt cctggcctac gcccggatgc
      421 ggggggacgc gtcggggcag atggtcgtct tcttcgtcat cgccgtcgcg gccgccgagg
      481 ccgcggtcgg gctcgcgatc ttcgtcgcgc tcttccgggc gaagcggacg atcgacgtcg
      541 acgagatcgc ctcgttgaag tggtgaggga gacgacgcga tgaaccttcc cctctggctc
      601 atcccggcgc tcccgctcgc cggcttcctc ctgaacggct ccgtcgccct cttctcgggc
      661 tggcggaggg cccggaaggc gaccgccgcg tggcgcgagg cccaccccgg cgaccacggg
      721 cacgacgagc acggcggcca cgccgccgac gaccacggcc acgacgacca cggacatcac
      781 ggccccttcc tcccgtacgg cgagaggctc ttctacggcg tcgtcggcgt cctctcggtc
      841 ggcctcgcga cgctcgtcgc gttcgcgaac gtcgtcccgt acgtcgcggc ctccctcgcg
      901 accgaggggg gcgtcggccc cgtcgtccag agcgtctgga agtggatggc ggcgggccac
      961 gtgtcgatcg acatggcgtt ccggctcgac ccgctgtcgg ccatgatggt gtcgttcgtc
     1021 accttcgtcg gcctcctcat ccacgtcttc agcgtcggct acatgcagga cgaggaaggg
     1081 tacgggcgct acttcgccta cctgaacctc ttcatgttcg cgatgctgac gctgatcctc
     1141 gcggacagcc tggtcgtcct gttcatcggc tgggaggggg tcgggctctg ctcctacctc
     1201 ctgatcggct actactacga caaggacttc gcccacgccg ccggcaagaa ggcgttcgtc
     1261 acgaaccgga tcggcgactt cgggatggtc ctcgggatct tcgggatcct cgccctcttc
     1321 gggacggcgg acttctcgtt cgtcgacatg gcaaaggccg gggcggtgaa cgggacggcc
     1381 tcgatcacgg tttacgccgt ctgcctcctc ctcttcctcg gcgcggtcgg caagagcgcg
     1441 caggtgccgc tctacgtctg gctccccgac gcgatggccg gcccgacgcc ggtctcggcc
     1501 ctgatccacg ccgcgacgat ggtcaccgcg ggcgtctacc tcgtcgcccg ctgctcggcc
     1561 gtcttcgtcc tcgcgcccga cgccctgacc ctcgtcgcct gggtcggcgc cgggacggcc
     1621 gtcttcgccg cgacgatcgg cctcgcccag aacgacatca agaaggtcct cgcctactcc
     1681 acggtctcgc agctcggcta catgttcctg gcgtgcgggg tcggggcgtt cggcgtcggg
     1741 atgttccacg tcttcaccca cgccttcttc aaggcctgcc tcttcctcgg ct
//
LOCUS       DAOTEZ010000639         1791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_59492_length_1791_cov_4.994240, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000639 DAOTEZ010000000
VERSION     DAOTEZ010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59492_length_1791_cov_4.994240"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="P5164_20295"
     CDS             <1..1044
                     /locus_tag="P5164_20295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRY46283.1"
                     /translation="TPTALVVGTGRGARMGLLLRDAGALEKAARVTAVVFDKTGTLTA
                     GRPAVTAVSPADGVSEADLLGTLAALESGSEHPLGKAIVAEANARGIVFGRARDVRAV
                     PGGGVTGTVGGAAALAGSEAFLAARGIAVPEAILGGADGGTLVLLATGGALRGAVTLA
                     DPLKPHAREALAELSARGLSLHLLSGDRPAAVLAAAREAGIPEANAAARLTPAEKAER
                     VMALRSAGAVVAMVGDGINDAPALAAADLGVAIGSGTDVARAASDVTLVGGALSGIGA
                     AIDLSNATLRNVRQNLFLAFVYNVVLIPLAAGAFYPFTGWTLNPMLAGFAMAASSVSV
                     VTNALRLRSFGRR"
     gene            complement(1054..>1791)
                     /locus_tag="P5164_20300"
     CDS             complement(1054..>1791)
                     /locus_tag="P5164_20300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HRY46284.1"
                     /translation="AGVCLLREPLPDAVLQSVAMEMNQAETAFLLPEGNDWRLRWFTP
                     EVEENLCGHATLASAHVLFETGLLAEDATARFQTRSGLLTAVRQGVSIVLDFPATPAE
                     PFPPLEKAVRALGVSPSFSGKTRFDLFFEVAEEAEVRTLRPDFALLEEVPYRGVIVTA
                     RAESGSAHDFVSRFFAPGCGVPEDPVTGSAHCALGPYWREKLGKDDLVGYQASARGGT
                     VQVGCLGDRVTLAGSAVTVVRGELVLP"
BASE COUNT          232 a          642 c          693 g          224 t
ORIGIN      
        1 actccgacgg cgctcgtcgt cgggacgggg cgcggcgcgc ggatggggct cctcctccgc
       61 gacgcggggg ccctcgagaa ggcggcccgc gtcacggcgg tcgtcttcga caagaccggc
      121 acgctcaccg cggggcggcc cgcggtgacg gcggtgagcc cggccgacgg cgtgagcgag
      181 gccgacctcc tcggcacgct cgcggccctc gagagcggct cggagcaccc gctcgggaag
      241 gcgatcgtcg cggaggcgaa cgcgcgcggg atcgtcttcg ggcgcgctcg cgacgtgagg
      301 gccgtgccgg gcggcggcgt gacggggacc gtcggcggcg cagccgctct cgccgggtcc
      361 gaggcgttcc tggccgcgcg ggggatcgcg gtgccggagg cgatcctcgg cggcgccgac
      421 ggagggacgc tcgtcctcct cgcgacgggc ggcgcgctcc gcggcgcggt gacgctcgcc
      481 gacccgctca agccccacgc gagggaggcg ctcgcggagc tctccgcccg cggcctctcg
      541 ctccacctcc tttcgggcga ccgcccggcg gcggtcctcg cggcggcgcg cgaggcgggg
      601 atcccggagg ccaacgccgc cgcgcgtctg acgccggcgg agaaggcgga gcgggtcatg
      661 gcgctccgct ccgcgggcgc ggtcgtggcg atggtcgggg acgggatcaa cgacgccccc
      721 gccctcgcgg ccgccgacct cggcgtggcg atcggctccg ggaccgacgt cgcccgcgcc
      781 gcctccgacg tcacgctcgt cggcggcgcc ctctcgggga tcggggcagc gatcgacctc
      841 tcgaacgcga cgctccgcaa cgtgaggcag aacctcttcc tcgcgttcgt ctacaacgtc
      901 gtcctgatcc cgctcgcggc gggcgcgttc taccctttca cgggatggac gctcaacccg
      961 atgctcgcgg gcttcgcgat ggcggcctcg tcggtcagcg tcgtgacgaa cgcgcttcgc
     1021 ctccggagct tcgggaggcg atgacgcgag atcctacggg aggacgagct cgccgcggac
     1081 gaccgtgacg gcggaaccgg cgagcgtgac ccgatcgccg aggcagccga cctggacggt
     1141 ccccccgcga gccgaggcct ggtagccgac gagatcgtct ttcccgagct tctccctcca
     1201 gtacggcccg agggcgcagt gcgccgagcc ggtgaccggg tcctcgggga cgccgcagcc
     1261 gggcgcgaag aagcgggaga cgaagtcgtg cgcgctcccg ctctcggcgc gagccgtgac
     1321 gatgacgccc cggtacggga cctcctcgag gagggcgaag tcggggcgga gcgtccggac
     1381 ctcggcttcc tcggcaacct cgaagaagag gtcgaagcgc gtcttccccg aaaaggaggg
     1441 ggagacgccg agcgcgcgga ccgccttctc gagcggcggg aagggctcgg ccggagtggc
     1501 cgggaagtcg aggacgatcg acaccccctg gcggacggcc gtcaggaggc cgctcctcgt
     1561 ctggaatcgc gccgtggcgt cctccgcgag gagaccggtc tcgaagagga cgtgcgccga
     1621 ggcgagcgtg gcgtgaccgc agaggttctc ctcgacctcg ggggtgaacc atctcagccg
     1681 ccagtcgttc ccctcgggga ggaggaaggc cgtctcggcc tggttcatct ccatcgccac
     1741 gctctgcagg acggcgtcgg ggagcggctc gcgaaggagg cagactccgg c
//
LOCUS       DAOTEZ010000640         1791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_59494_length_1791_cov_4.739055, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000640 DAOTEZ010000000
VERSION     DAOTEZ010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59494_length_1791_cov_4.739055"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="P5164_20305"
     CDS             <1..194
                     /locus_tag="P5164_20305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46285.1"
                     /translation="ETIEALPPTALALLAGAREQGETGIADVEARLVAEEIVPFDRPV
                     SGAVRIDVDVERPRSRGSR"
     gene            191..>1791
                     /locus_tag="P5164_20310"
     CDS             191..>1791
                     /locus_tag="P5164_20310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46286.1"
                     /translation="MNLTSNRALAAGLALALVLPAAPPLLAATRLSTFGSARDFLAGE
                     LGGTTLTSDGRLTLGPVFSSPAWPEDAAGAVVFAAASDASGRVFVATGGGLGRLFVSE
                     PDGSVRVLFEADAPNVTAVAVGPRGEVVCGTSPGGRIWRVDPKAKDPSKAGLLAGETG
                     EAAIWALAVGMDGTIWAGTGSKGRIWKAGADGRASLHAELEDTHVRSLLLRGDGSLVA
                     GTSDRGLVVAVAPDGTVRTLHDFGRPEVVALAPAPEGGVYAVATSVAVPTLAQQRSEP
                     RQTPAPSGAGPAAAPGGASEPVPQGSVSVSSTTSPVRPPAAATSGREGNAEVVLVAAD
                     GFVEPAWILPEETAYGARWDEGRGALLLATGPKGRVYSLKGRQLRLEVQAGERQVVAA
                     PAVPGGFAIVASSSPAILRPAASSVPGRGTYVSAVRDAGRMARFGAIRLEGTVPKGAA
                     ALLSVRSGNSDSPDGTWSAWTGTGGAGSVTVPPARYFQWRVELAASPRGEAPVIERVE
                     TSWSERNARPIVENVSVLEPGAVFAR"
BASE COUNT          206 a          669 c          679 g          237 t
ORIGIN      
        1 gcgagacgat cgaggcgctg ccgccgacgg ctctcgccct cctcgccggc gcccgggagc
       61 agggagagac cgggatcgcc gacgtcgagg cccggctcgt cgccgaggag atcgtcccct
      121 tcgaccgccc cgtctcgggc gccgtccgca tcgacgtcga cgtcgagagg ccccgctcgc
      181 gaggatcccg atgaacctga cctcgaaccg agctctcgcc gcgggcctcg cgctcgccct
      241 cgtccttccc gcggcgccgc cgctcctcgc cgcgacgcgt ctctcgacgt tcgggtcggc
      301 ccgcgacttc ctcgccggcg agctcggcgg gacgacgctg acgtcggacg ggcgcctgac
      361 gctcggcccc gtcttctcgt ccccggcgtg gccggaagac gccgccggag ccgtcgtctt
      421 cgccgccgcg tcggacgcct cgggccgggt cttcgtggcg accgggggcg ggctcggacg
      481 gctcttcgtc tccgagccgg acggctcggt acgcgtcctc ttcgaagcgg acgctccgaa
      541 cgtcaccgcc gtcgccgtcg ggccgcgtgg cgaggtcgtc tgcgggacct cgccgggagg
      601 ccggatctgg agggtcgatc cgaaggcgaa ggacccctcg aaggccggcc tgctcgccgg
      661 cgaaacggga gaggccgcga tctgggcgct ggctgtcggg atggacggga ccatctgggc
      721 cgggaccggt tcgaagggac ggatctggaa ggcgggcgcc gacggaaggg cctcgctcca
      781 cgccgagctc gaggacacgc acgtccgctc gctcctgctg cgcggcgacg gctcgctcgt
      841 ggcggggacc tccgaccggg gactcgtcgt cgccgtcgcg cccgacggca cggtacgcac
      901 gctccacgac ttcggccggc cggaggtcgt ggccctcgcg ccggcgcccg agggaggtgt
      961 ctacgctgtc gcgacctccg tggcggttcc gacgctggcc cagcagagaa gcgagccccg
     1021 gcagacgccc gcgccgtccg gcgccgggcc cgcggcggct ccgggtgggg cgagcgaacc
     1081 ggtcccgcag ggaagcgtct cggtctcctc gacgacgtcg ccggtgaggc ctcccgccgc
     1141 cgcgacctcc gggcgggaag ggaacgccga ggtcgtcctc gtcgctgcgg acgggttcgt
     1201 cgagccggcc tggatcctcc ccgaggagac ggcttacggg gcacgctggg acgaagggcg
     1261 cggggcgctc ctcctcgcga cgggcccgaa gggacgcgtc tactctctga aggggcgcca
     1321 gctgcgcctc gaggtgcagg cgggagagcg acaggtcgtc gccgcgcccg cggttccggg
     1381 cggattcgcg atcgtggcgt cgagctctcc cgccatcctc cgaccggcgg cttcgagcgt
     1441 cccgggacgc gggacgtacg tttccgcggt ccgcgacgcg ggccgaatgg cccgattcgg
     1501 cgcgatccgg ctcgagggga cggtgccgaa aggcgcggcg gccctcctct cggtccgctc
     1561 cgggaacagc gactcgcccg acggcacgtg gtccgcgtgg accgggaccg ggggcgccgg
     1621 gagcgtcacc gtccctccgg cgcgctactt ccagtggcgc gtcgagctcg cggcctcgcc
     1681 gcgcggcgag gcgcccgtca tcgagcgggt ggagacctcc tggtccgagc gaaacgcccg
     1741 gccgatcgtc gagaacgtct ccgtcctcga gccgggagcg gtcttcgccc g
//
LOCUS       DAOTEZ010000641         1789 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_59615_length_1789_cov_6.316032, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000641 DAOTEZ010000000
VERSION     DAOTEZ010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59615_length_1789_cov_6.316032"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="P5164_20315"
     CDS             <1..218
                     /locus_tag="P5164_20315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HRY46287.1"
                     /translation="EDGKLTTPIKDVNIIGNGPKVLEKIDMVADDLVIDEGGWTCGKD
                     GQSVPVSQGIPTVRVASITVGGRGKQA"
     gene            229..1557
                     /locus_tag="P5164_20320"
     CDS             229..1557
                     /locus_tag="P5164_20320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HRY46288.1"
                     /translation="MAQQDMLAAAHAAVKSALGKGAQEASARTYRVREVEVKWRDGSL
                     EKVHEATTRGLGLTLYVDGRYSNVSTSDLRPEALETFIGDSVTLTRALAKDPFRTLPD
                     PKLYEGQAKVDLLLEDPKYATVTPEMRRAVAKEIEAAARSVQGAKSILSVTTGFSDTL
                     AESWRVSSNGFSGSRRDTQFWVSAEVSVKDPDGRKPEDYAFGGSRFVGEMPKAGEIGR
                     DAAARALSRLGAKKGESAVLTMAVENRAAGRLPAYLLGPLSGGALQQKRSFLEGKLGQ
                     AIASPALTLVDDPLVPKGFGSRLFDGEGIAAKRMPIVEAGVLKSYFIDTYYGKKLQMA
                     PTTGGPSNVVWTLGDKDQAALLAAMKEGILVTGFLGGNSNGLTGDFSLGVQGFRVRGG
                     KIAEPVGEMNISGNHLDLWKKLAAVGNDPYPYSSMKTPTLVFEGVQFAGV"
BASE COUNT          294 a          653 c          613 g          229 t
ORIGIN      
        1 tcgaggacgg caagctgacg accccgatca aggacgtgaa catcatcggg aacggaccga
       61 aggtcctgga gaagatcgac atggtcgcgg acgacctcgt catcgacgag gggggctgga
      121 cgtgcggcaa ggacggccag tcggtcccgg tctcccaggg aatccccacc gtgcgcgtcg
      181 cgtcgatcac cgtcggcgga cgcgggaagc aggcgtgagg tgagagagat ggcacagcag
      241 gacatgctcg ccgccgccca cgccgccgtg aagagcgccc tcggcaaggg ggcgcaggaa
      301 gcctccgccc gcacttaccg cgtccgcgag gtcgaggtga agtggcgcga cgggagcctc
      361 gagaaggtcc acgaggcgac gacccgcggc ctcggcctca cgctctacgt cgacggccgc
      421 tactcgaacg tctcgacgag cgacctccgc ccggaggcgc tcgagacgtt catcggcgac
      481 tccgtcaccc tcacccgcgc cctcgcgaag gacccgttcc ggacgctccc cgacccgaag
      541 ctctacgagg ggcaggcgaa ggtcgacctc ctcctcgagg acccgaagta cgcgaccgtc
      601 acgcccgaga tgaggcgcgc cgtcgcgaag gagatcgagg cagcggcccg atcggtccag
      661 ggcgccaagt cgatcctctc cgtcacgacc ggcttcagcg acacgctcgc cgagagctgg
      721 cgcgtctcct cgaacggctt ctccggctcg cgccgtgaca cgcagttctg ggtctcggcc
      781 gaggtgagcg tcaaggaccc cgacgggcgc aagcccgagg actacgcgtt cggcggctcg
      841 cgcttcgtcg gcgagatgcc gaaggcgggc gagatcggtc gcgacgccgc cgcgcgcgcc
      901 ctctcgcgcc tcggcgcgaa gaagggcgag tcggccgtcc tcacgatggc ggtcgagaac
      961 cgcgccgccg gccggctccc ggcctacctc ctcggcccgc tcagcggcgg cgcgctccag
     1021 cagaagcgct ccttcctcga ggggaagctc ggccaggcga tcgcgagccc cgctctcacg
     1081 ctcgtcgacg acccgctcgt cccgaagggc ttcggctcgc gcctcttcga cggcgagggg
     1141 atcgccgcga agcggatgcc gatcgtcgag gcgggcgtcc tcaagtcgta cttcatcgac
     1201 acttactatg ggaagaagct ccagatggcg ccgacgaccg gcgggccgtc gaacgtcgtc
     1261 tggacgctcg gcgacaagga ccaggccgcc ctcctcgccg cgatgaagga ggggatcctc
     1321 gtcaccggct tcctcggcgg caactcgaac ggcctcacgg gcgacttctc cctcggcgtc
     1381 cagggcttcc gcgtccgcgg cggcaagatc gccgagccgg tcggcgagat gaacatctcc
     1441 ggcaaccacc tcgacctctg gaagaagctc gccgccgtcg gcaacgaccc ctacccctac
     1501 tcctccatga agacccccac cctcgtcttc gagggcgtcc agttcgccgg ggtctgaccg
     1561 aaagacgccg ccgagagcga ccgcgaagag aaccaccgag ggccggtccg gaagggccgg
     1621 cccttttctc ttctgcggac ggcgtcgcgc aggcgacgag ggacgggaga ggcgatccgt
     1681 ccacttcggc gtgccgctcg agtcgcgggc gggaggggca tcccacggct cgccctgcgc
     1741 gcgcgcgacc cgcatcggcc ccgctgcgcg ggccgccggc gagcgcgca
//
LOCUS       DAOTEZ010000642         1788 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_59670_length_1788_cov_5.224466, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000642 DAOTEZ010000000
VERSION     DAOTEZ010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59670_length_1788_cov_5.224466"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="P5164_20325"
     CDS             <1..857
                     /locus_tag="P5164_20325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HRY46289.1"
                     /translation="RFQPGDYKSDLKPVWCAGCGDFGVVSAMYRAMAELQLEPWNTVV
                     VSGIGCSSRLPGYVEAYGFNSVHGRALPIATGIKVARPELNVVAVGGDGDGLAIGGNH
                     FLHASRRNLDVFYVLMDNEIYGLTKGQAAPTTPSGDKTKSTFFGNPEPSVDPCELAIS
                     FGATWVARGFSGDMKGLVDLMVAGMSHRGFAFLNVMSPCVTWRGDDQFKVMKAKLRSL
                     PEGYDRSSRAAAFAYTRETETLTTGVLYEAHEPSLLDRLEAQRTKAMEGHEGLTTEKV
                     LKVFFPPF"
     gene            complement(1006..>1788)
                     /locus_tag="P5164_20330"
     CDS             complement(1006..>1788)
                     /locus_tag="P5164_20330"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRY46290.1"
                     /translation="DWRINGGAWTATAATSAIAPPRGAVDPVQLFVRAFACDPEKGPG
                     AEASSPVYSLAPPQASFAVPSSVVAGQPATFTDTSSPQATSWLWFPGDGMPATTVQSP
                     TVTFPSAGPRVIVLVATNGSGTSTKSTTVNVLPASAVRTAAGSFVRRLDRQPDGRLAL
                     DEVDVARGTTLLLRRLSGAGDAVAFLRLVDTSGAVAVERRLVLAEGEEARHELFAWGG
                     GRFRVELVGPDGLEATVVTSAAPAPAPELPVPPREPRRIEPR"
BASE COUNT          266 a          654 c          629 g          239 t
ORIGIN      
        1 cccgcttcca gccgggcgac tacaagagcg acctcaagcc cgtctggtgc gccgggtgcg
       61 gcgacttcgg cgtcgtctcc gcgatgtacc gcgcgatggc ggagctgcag ctggagccct
      121 ggaacaccgt cgtcgtctcc ggcatcggct gctcctcgcg gctgcccggc tacgtcgagg
      181 cctacggctt caacagcgtc cacggccgtg ccctcccgat cgcaacgggc atcaaggtcg
      241 cccgccccga gctgaacgtc gtggcggtcg gcggcgacgg agacggcctg gccatcggcg
      301 gcaaccactt cctccatgcc tcccgccgga acctcgacgt cttctacgtc ctgatggaca
      361 acgagatcta cgggctcacg aaggggcagg cggctcccac gacgccctcg ggcgacaaga
      421 cgaagtcgac cttcttcggg aaccccgagc cgagcgtcga cccgtgcgag ctcgcgatct
      481 ccttcggggc gacctgggtc gcccgcggct tctcgggcga catgaagggt ctcgtcgacc
      541 tcatggtcgc cggcatgagc catcgcggct tcgcgttcct gaacgtgatg tccccctgcg
      601 tcacctggcg cggggacgac cagttcaagg tgatgaaggc gaagctccgg agccttcccg
      661 aggggtacga ccgtagcagc cgggccgccg ccttcgccta cacgcgcgag acggagaccc
      721 tcacgacggg cgtcctctac gaggcgcacg agccctcgct cctcgaccgg ctcgaggcgc
      781 agaggacgaa ggcgatggaa ggtcacgaag ggctgacgac cgagaaggtc ctgaaggtgt
      841 tcttcccgcc gttctgagcg ctccccgcgc tcggcggagc cggcgcgcgg agaccgacga
      901 tcccggcgaa gagcccgggc cgaatggccc gggctctttc tctacctgct ccgcattgga
      961 gggcgcggga aggttcgtgg cgagcctccc cgatccgtcc tcacctcacc tcggctcgat
     1021 gcgcctgggc tcccgcggcg gcacggggag ctccggagcc ggcgccggcg cagccgaggt
     1081 cacgaccgtc gcctcgagcc catcggggcc gacgagctcg acccggaagc ggccgccgcc
     1141 ccaggcgaag agctcgtgac gcgcctcctc gccctcggcg aggacgaggc gtctctcgac
     1201 ggcgaccgca ccggacgtgt cgacgaggcg gaggaacgcg accgcatccc ccgctcccga
     1261 gagccggcga aggaggagcg tcgtcccccg ggcgacgtcg acctcgtcga gcgcgagacg
     1321 gccgtcgggc tggcggtcga gcctccggac gaacgagccc gccgccgtcc gcacggccga
     1381 agccggcagg acgttcaccg tcgtcgactt cgtcgaggtg ccggagccgt tcgtcgcgac
     1441 gaggacgatc acccgcggcc ctgccgacgg gaacgtgacc gtcggcgact ggaccgtggt
     1501 cgccggcatt ccgtcgcccg ggaaccagag ccagctcgtc gcctgcggcg aggaggtgtc
     1561 ggtgaacgtg gccggctggc cggcgacgac cgacgacgga acggcgaagc tcgcctgcgg
     1621 cggcgcgagc gaatagacgg ggctggacgc ctccgccccc ggccccttct cggggtcgca
     1681 ggcgaacgcg cgcacgaaga gctggaccgg atcgaccgcg cctcgcggcg gtgcgatcgc
     1741 ggaggtcgcg gcggtggccg tccacgcccc tccgttgatc cgccagtc
//
LOCUS       DAOTEZ010000643         1787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_59720_length_1787_cov_5.977483, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000643 DAOTEZ010000000
VERSION     DAOTEZ010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59720_length_1787_cov_5.977483"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..1676)
                     /gene="malQ"
                     /locus_tag="P5164_20335"
     CDS             complement(150..1676)
                     /gene="malQ"
                     /locus_tag="P5164_20335"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRY46291.1"
                     /translation="MRTRAAGLLLHPTSLPGRFGIGDLGPECDAFLDWAASAGQSVWQ
                     VLPLGPTGFGNSPYGTLSAFAGSPMLLSPELLVADGLLPASALDGAPGFPEHRVDFER
                     VIPWKEGLLRESWRRFRTDAPADLRAGLQGFAEASAHGYWLRDWALFMALKGRYGGAA
                     FREWPEELRLRRSAALARAERELAEETSWHAYLQYLFFRQWERVKDAAHARGVRILGD
                     VPIYVSGDSADVWAHPEIFELDAEGDPVRVAGVPPDYFSETGQLWGNPLYRWDVLERD
                     GYRWWVERMRANLRLTELLRLDHFRGFAGYWAVPADAETAIAGSWQEGPGRKLFDALA
                     AELGELDLVAEDLGEITPDVKALVAALDLPGMKILQFAFGGDDEDYQPHNHAPNGVVY
                     TGTHDNDTTRGWWEKADDATRHRVRAYLGGDGTDPIGTFVRAAYASVARLAILPVQDV
                     FGLGSEARMNTPGKGADNWAYRARKWDFNGERAAWLRELATLTGRFRPAPAGNGPNGG
                     "
BASE COUNT          246 a          654 c          650 g          237 t
ORIGIN      
        1 cggaggcgga cctctacctc ttcgtcgccc ggcacctcca cgcgctccgg gagcgctggg
       61 gcgacggtgt cgcgccggcc cgcgcggtga agcacctccg cctccagacg aagcggagcc
      121 ggtcgcggaa cgcgaagggc tgagcggggt cagccgccgt tcgggccgtt cccggccggg
      181 gccggacgga agcgtcccgt cagcgtcgcg agctcgcgca gccaggcggc gcgctccccg
      241 ttgaagtccc acttccgcgc ccggtacgcc cagttgtcgg cccccttccc cggcgtgttc
      301 atccgcgcct cgctcccgag gccgaagacg tcctgcaccg ggaggatggc cagccgtgcc
      361 accgaggcgt aggccgccct cacgaaggtc ccgatcgggt cggtcccgtc gcctccgagg
      421 taggcgcgca cgcgatgacg cgtcgcgtcg tccgccttct cccaccagcc gcgggtcgtg
      481 tcgttgtcgt gcgtcccggt gtagaccacg ccgttcgggg cgtggttgtg cggctggtag
      541 tcctcgtcgt ctccgccgaa cgcgaactgg aggatcttca tgccgggcag gtccagcgcg
      601 gccacgagcg ccttcacgtc gggcgtgatc tcgccgaggt cctccgcgac gaggtcgagc
      661 tccccgagct cggcggcgag ggcgtcgaac agcttccggc ccggcccctc ctgccaggag
      721 ccggcgatcg ccgtctcggc atcggcgggg acggcccagt agccggcgaa gccgcggaaa
      781 tggtcgaggc ggaggagctc cgtgagccgg aggttcgccc tcatccgctc gacccaccag
      841 cggtagccgt cccgctcgag gacgtcccag cggtagagcg ggttccccca gagctggccg
      901 gtctcgctga agtagtcggg cgggacgccc gcgacccgga ccgggtcccc ctccgcgtcg
      961 agctcgaaga tctcggggtg cgcccagacg tcggcgctgt cgccggagac gtagatcggg
     1021 acgtcgccca ggatccggac gccccgcgcg tgggcggcgt ccttcaccct ctcccactgc
     1081 cggaagaaga ggtactggag gtacgcgtgc caggacgtct cctccgcgag ctccctctcg
     1141 gcgcgcgcga gggctgcgga cctccggagc cggagctcct ccggccactc gcggaaggcg
     1201 gccccgccgt agcgtccctt cagcgccatg aagagcgccc agtcgcggag ccagtagccg
     1261 tgcgccgaag cctccgcgaa gccctgcagc ccggcccgca ggtccgccgg agcgtcggtc
     1321 cggaagcgcc gccacgactc gcgcaggagc ccctccttcc aggggatcac gcgctcgaag
     1381 tcgacgcggt gctcggggaa cccgggggcc ccgtcgagcg ccgaggcggg gaggaggccg
     1441 tcggcgacga gaagctcggg cgacaagagc atcgggctgc cagcaaaggc cgagagcgtc
     1501 ccgtacggcg agttcccgaa gccggtcggg ccgagaggca gcacctgcca gaccgactgg
     1561 ccggcggagg cggcccagtc gaggaaggcg tcgcattccg gcccgaggtc gccgatcccg
     1621 aaccggcccg gcagggacgt cggatggagg aggaggcccg cggcgcgcgt tctcatccgg
     1681 aggatcctat ccgttgccgg caacgcttcg ccggctgcct ccgtaaaaga gtgcggggaa
     1741 tgaaacgggg acgactctcc cggctcgcgc tcgcggtggt gctcctg
//
LOCUS       DAOTEZ010000644         1779 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_60161_length_1779_cov_5.889791, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000644 DAOTEZ010000000
VERSION     DAOTEZ010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60161_length_1779_cov_5.889791"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="P5164_20340"
     CDS             <1..789
                     /locus_tag="P5164_20340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methylcrotonyl-CoA carboxylase"
                     /protein_id="HRY46292.1"
                     /translation="QRRHQKVVEESPSPAVLPELRARMSEAAVAAAKAVGYVNAGTVE
                     FLLDADGSFYFLEMNTRIQVEHPVTEEAWGLDLVTLQLAVAAGEPLPPDLPAVPSAHA
                     IEARVYAEDPENGFLPQVGTVLAYEEPGGPGVRVDSGIEAGSEVSVHYDPMLAKVVAR
                     GRTREEARLRLVDALGRTVVLGVATNVSWLRRLLQTPEFVRGELDTSLLGRLEVPAPP
                     DPPPDVLSAAARLLGGGDGRAAMTAPARAAFPDPFADGSFRVLG"
     gene            786..1172
                     /locus_tag="P5164_20345"
     CDS             786..1172
                     /locus_tag="P5164_20345"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46293.1"
                     /translation="MRIRDLSTGVVHEPPSGAGDLPRGARVVRDGFVAWVWHRGETWR
                     LGRVVEKPGAAEEEEHDLRAPMPGVVGRVLAEEGQEVVKGTPLLVLTAMKMEHEIRSP
                     RPGKLVKLFRTAGERVDLGDPLAEIE"
     gene            1169..>1779
                     /locus_tag="P5164_20350"
     CDS             1169..>1779
                     /locus_tag="P5164_20350"
                     /EC_number="4.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004419 - hydroxymethylglutaryl-CoA lyase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HRY46294.1"
                     /translation="MTAPGRVTVVEVGPRDGLQNEKGFVSTAVKVAFVEALAEAGLPV
                     VETTAFVSPRAIPQLADSAEVFGRVQKRPGTRYPVLVPNERGMARALAAGAREIALFT
                     AATDTFNVRNVNATVEESFARFAPVLARAKGEGLRVRGYVSTAFGCPYEGKVAPARAA
                     LVAARLFAAGCEEVSLGDTIGVAVPSQVTDVLGRTTEAGVPIER"
BASE COUNT          223 a          659 c          653 g          244 t
ORIGIN      
        1 cagaggcggc accagaaggt cgtcgaggag agcccctcgc ccgccgtctt gccggagctc
       61 cgcgcgcgaa tgagcgaagc ggccgtcgcg gcggcgaagg ccgtcggata cgtgaacgcc
      121 ggcacggtcg agttccttct cgacgccgac ggctcgttct acttcctcga gatgaacacc
      181 cgcatccagg tggagcaccc ggtcacggaa gaggcgtggg gcctcgacct cgtgacgctc
      241 cagctcgcgg tcgccgcggg ggagccgctc ccgccggacc tgccggcggt gccttcggcg
      301 cacgcgatcg aggcgcgcgt ctacgcggaa gaccccgaga acggcttcct cccgcaggtc
      361 gggacggtcc tcgcctacga ggagccgggc ggtcccggcg tccgcgtcga ttccgggatc
      421 gaggcgggaa gcgaggtttc cgtccactac gacccgatgc tcgcgaaggt cgtcgcccgc
      481 gggcggaccc gcgaggaggc tcgcctgcgc ctcgtcgacg cgctcgggcg gacggtcgtc
      541 ctcggcgtcg cgacgaacgt ctcctggctt cgccggctcc ttcagacgcc ggagttcgtg
      601 cgcggcgagc tcgacacgtc gctcctcggc cggctggagg tgccggctcc tcccgatcct
      661 ccgccggacg tcctctccgc ggcggcccgc ctcctcggag ggggcgatgg acgcgccgcg
      721 atgacggcgc cggcccgggc ggccttcccc gatccgttcg ccgacggttc cttccgggtg
      781 ctcggatgag aatccgcgac ctatcgaccg gcgtcgtcca cgagcctccg tccggcgcgg
      841 gggacctgcc ccggggcgcg cgcgtcgtcc gcgacggctt cgtcgcctgg gtctggcatc
      901 ggggggagac gtggcgcctc gggcgcgtcg tcgagaagcc cggcgcggcc gaggaggagg
      961 agcacgacct gcgcgccccg atgccgggcg tcgtcggccg cgtcctcgcg gaggaagggc
     1021 aggaggtcgt caaggggacg ccgctcctcg tcctgacggc gatgaagatg gagcacgaga
     1081 tccggagccc gcgccccggg aagctcgtga agctcttccg gacggcgggc gagcgcgtcg
     1141 acctgggcga cccgctcgcg gagatcgagt gacggctccg ggccgcgtca ccgtcgtcga
     1201 ggtcggcccg cgcgacggcc tccagaacga gaaggggttc gtctcgaccg ccgtgaaggt
     1261 cgccttcgtc gaggcgctcg ccgaggcggg cctccccgtc gtggagacga cggcgttcgt
     1321 ctcgccccgg gcgatcccgc agctggccga ctcggccgag gtcttcggcc gcgtgcagaa
     1381 gaggcccggg acccgctacc cggtcctcgt cccgaacgaa cgggggatgg cccgcgccct
     1441 cgccgccggc gcccgcgaga tcgctctctt caccgcggcg acggacacct tcaacgtccg
     1501 caacgtcaac gcgacggtcg aggagtcctt cgcccgcttt gccccggtcc tcgcccgggc
     1561 gaagggggag ggcctccgcg tccgaggcta cgtctcgacg gccttcggct gcccctacga
     1621 gggaaaggtc gcccccgcca gggcggccct cgtcgccgcg cgcctcttcg ccgccggctg
     1681 cgaggaggtg tccctcggcg acacgatcgg agttgccgtc ccgtcccagg tcaccgacgt
     1741 cctcggcagg acgacggagg ccggcgtccc gatcgagcg
//
LOCUS       DAOTEZ010000645         1778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_60223_length_1778_cov_6.300058, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000645 DAOTEZ010000000
VERSION     DAOTEZ010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60223_length_1778_cov_6.300058"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="P5164_20355"
     CDS             complement(<1..182)
                     /locus_tag="P5164_20355"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRY46295.1"
                     /translation="MSRIGRAFSRAARDGRAALVVFVEAGDPSLEVTERLLPALAAAG
                     ADVIELGVPFSDPIADG"
     gene            complement(179..1390)
                     /gene="trpB"
                     /locus_tag="P5164_20360"
     CDS             complement(179..1390)
                     /gene="trpB"
                     /locus_tag="P5164_20360"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRY46296.1"
                     /translation="MAEKFVVPEADAAGRFGEFGGRFVPETLMAPVEELTKAYAKARA
                     DASFRRELHHLLTTYAGRPTPLTEAKRLSEELGARILLKREDLLHTGAHKINNALGQV
                     LLAKRMGKSRVIAETGAGQHGVATATAAALLGIPCVVYMGSVDVERQALNVYRMRLLG
                     TKVVPVDSGSKTLKDAINEAMRDWVASYADTHYVLGSALGPHPYPRMVRDFHAVIGRE
                     ARAQARKLLGGLPDLVVACVGGGSNAIGLFHAFLPEREVELVGVEAGGRSSAPGEHAA
                     RFGPGRVGVLHGTRTLLLADDDGQVLPTHSVSAGLDYPAVGPEHANLAALGRTRYESV
                     TDDEALAAFHALSEKEGIIPALESAHAVAWVLREAPKRRGGTILLGLSGRGDKDVPTV
                     ARLEGAAGVAL"
     gene            complement(1383..>1778)
                     /locus_tag="P5164_20365"
     CDS             complement(1383..>1778)
                     /locus_tag="P5164_20365"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRY46297.1"
                     /translation="DALQWHDDLAAREGLRVPVPVWQAVRVGRDLLEEAASWSADVLL
                     LDAAHATLAGGTGLAWDWSLLEHFPAKEKTFVAGGLSPENVARAIAACAPAGVDVASG
                     VESAPGIKDGAKLAAFLAAVRSEKGRIGG"
BASE COUNT          233 a          666 c          650 g          229 t
ORIGIN      
        1 ccgtccgcga tcgggtcgga gaacggcacg ccgagctcga tcacgtcggc gccggcggcg
       61 gcgagggccg ggagcagacg ctccgtgacc tcgagcgacg ggtcgcccgc ctcgacgaag
      121 acgaccaggg cggcgcggcc gtcgcgcgcg gcacgcgaga aggcccgacc gatgcggctc
      181 acagggccac ccccgcggcg ccctcgagcc gcgcgacggt cggcacgtcc ttgtcgccgc
      241 ggccggagag gccgaggagg atcgtcccgc cgcgccgctt cggcgcctcg cggaggaccc
      301 acgcgaccgc gtgggcgctc tcgagcgccg ggatgatccc ctccttctcg gaaagggcgt
      361 ggaaggcggc gagcgcctcg tcgtcggtga cgctctcgta gcgggtccgg ccgagcgcgg
      421 cgaggttcgc atgctcgggc ccgaccgccg ggtagtcgag cccggcggag accgagtggg
      481 tcggaaggac ctggccgtcg tcgtcggcga ggaggagcgt ccgcgttccg tgcaggacgc
      541 cgacgcggcc cgggccgaag cgggccgcgt gctcccccgg cgcggaagag cggccgcccg
      601 cctcgacgcc gacgagctcg acctcccgtt cggggaggaa ggcgtggaag aggccgatcg
      661 cgttcgagcc gccgccgacg caggcgacga cgaggtcggg gaggcccccg aggagcttcc
      721 tcgcctgcgc gcgcgcctcg cggccgatga cggcgtggaa atcgcggacc atccgcgggt
      781 acgggtgcgg cccgagggcc gagccgagga cgtagtgcgt gtcggcgtag ctcgccaccc
      841 agtcgcgcat cgcctcgttg atcgcgtcct tgagcgtctt cgacccggag tcgacgggga
      901 cgactttcgt tccgagaagc ctcatccggt agacgttcag cgcctggcgc tcgacgtcga
      961 cggagcccat gtagacgacg cacgggatcc cgaggagcgc ggccgccgtc gccgtcgcga
     1021 cgccgtgctg gccggccccg gtctccgcga tgacgcggct ctttcccatc cgcttcgcga
     1081 ggagcacctg cccgagcgcg ttgttgatct tgtgcgcccc cgtgtggagg aggtcctcgc
     1141 gcttgaggag aattcgcgcc ccgagctcct ccgagaggcg cttcgcctcg gtgagcggcg
     1201 tcggccggcc cgcgtacgtc gtcaggaggt ggtggagctc gcggcggaac gacgcgtcgg
     1261 cgcgcgcctt cgcgtacgcc ttcgtcagct cctcgacggg cgccatcagg gtctcgggga
     1321 cgaaccgccc gccgaactcg ccgaaccgcc ccgccgcgtc ggcctcggga accacgaact
     1381 tctcagccac cgatcctccc cttctccgac cggaccgcgg cgaggaacgc cgcgagcttc
     1441 gctccgtcct tgattcccgg tgcgctctcg acgcccgagg ccacgtcgac gcccgccggc
     1501 gcgcacgccg cgatcgccct cgcgacgttc tccggcgaga gcccacccgc gacgaaggtc
     1561 ttctccttcg ccgggaagtg ctcgaggagg ctccagtccc atgcgaggcc cgtccctccc
     1621 gcgagcgtcg cgtgcgcggc gtcgaggagg aggacgtccg cggaccacga cgcggcctcc
     1681 tcgagcaggt ccctccccac ccgaacggcc tgccacacgg gaacggggac gcgcagcccc
     1741 tcccgcgccg cgaggtcgtc gtgccactgg agcgcgtc
//
LOCUS       DAOTEZ010000646         1769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_60796_length_1769_cov_6.757293, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000646 DAOTEZ010000000
VERSION     DAOTEZ010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60796_length_1769_cov_6.757293"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="P5164_20370"
     CDS             complement(<1..443)
                     /locus_tag="P5164_20370"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY46298.1"
                     /translation="MSRVAGRTRVPALVIGTGIAGLTAALALAKRGIRVLLLTKAAEP
                     DDCNTRYAQGGIIYFGKRDSPSALVRDVLAAGAGLCNAEAVRFLAQEGPRVVEEVLIG
                     DAGVPFTRTRKGDLDFTREGAHSVARIVHSADATGKAIELALLARV"
     gene            complement(440..1342)
                     /gene="nadC"
                     /locus_tag="P5164_20375"
     CDS             complement(440..1342)
                     /gene="nadC"
                     /locus_tag="P5164_20375"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRY46299.1"
                     /translation="MKTDRRLVEALDDLLVRAFAEDGEDVTTLAVFGPKARVEADMVC
                     RDAAVVAGVAFLPRVFAFLHPPARVELRVDDGTKVAPGTVLARVEGPAISVLAAERTA
                     LNLVQRLTGVATATRAWVEALEGSGTRLLDTRKTTPGLRALEKYAVRCGGGTNHRMGL
                     SDAFLVKDNHADGCGSLWDATRRAADFRAINRSLKRCLLEAEARTRDEVHQALEAGAE
                     RVLLDNMTVAAMREAVRDVKLWNEATGAAVTTEASGGMTIEKARKAARAGVDFVSVGA
                     ITHSVRAADVALDVKMAATRRGRR"
     gene            complement(1534..>1769)
                     /locus_tag="P5164_20380"
     CDS             complement(1534..>1769)
                     /locus_tag="P5164_20380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46300.1"
                     /translation="LPEAGGALVGNPLPEWATRLAALRIGSSGDPARAGSEIERLRRE
                     AALVLPARERRRYAALAHWLAWTARGGGRAASR"
BASE COUNT          223 a          631 c          685 g          230 t
ORIGIN      
        1 acgcgggcga ggagggcgag ctcgatcgcc ttcccggtgg cgtcggcgga gtggacgatc
       61 cgggcgacgg agtgcgcccc ctcgcgggtg aagtcgaggt ctcccttccg ggtgcgggtg
      121 aaggggaccc cggcgtcgcc gatcaggacc tcctccacga cgcgcggccc ctcctgcgcg
      181 aggaagcgga ccgcctcggc gttgcagagg ccggcgccgg cggcgaggac gtcccggacg
      241 agcgcggagg gggaatcgcg cttgccgaag tagatgatcc cgccctgggc gtagcgcgtg
      301 ttgcagtcgt ccggctctgc cgccttcgtc aggaggagga cgcggatgcc ccgcttggcc
      361 agcgcgagag cggccgtcag cccggcgatc ccggtcccga tgacgagcgc cgggacgcgg
      421 gtccgcccgg cgacccggct caccggcggc ccctccgggt cgcggccatc ttcacgtcga
      481 gggcgacgtc ggcggcgcgc acggagtgcg tgatcgcgcc gacggagacg aagtcgacgc
      541 cggcgcgagc ggccttccgg gccttctcga tcgtcatccc gcccgaggcc tcggtcgtga
      601 ccgcggcccc cgtggcctcg ttccagagct tcacgtcgcg gaccgcctcg cgcatcgcgg
      661 cgacggtcat gttgtcgagg aggacccgct cggcgcccgc ctcgagcgcc tggtggacct
      721 cgtcgcgcgt ccgcgcctcg gcctcgagga ggcagcgctt cagcgagcgg ttgatcgcgc
      781 ggaagtcggc cgctcggcgg gtcgcgtccc agaggctccc gcagccgtcg gcgtggttgt
      841 ccttcacgag gaaggcgtcg gagagcccca tccggtgatt cgtcccgccg ccgcagcgga
      901 cggcgtactt ctcgagcgca cgcaggcccg gcgtcgtctt gcgcgtgtcg aggagccgcg
      961 tgccgctccc ctcgagcgcc tcgacccagg cgcgcgtcgc cgtggcgacc ccggtgaggc
     1021 gctggacgag gttgagcgcg gtccgctcgg ccgcgaggac cgagatggcg ggcccttcca
     1081 cgcgcgcgag gacggtgccg ggcgccacct tcgtcccgtc gtcgacccgg agctcgacac
     1141 gggcgggggg gtggaggaac gcgaagacgc gcgggaggaa ggcgacgccg gcgacgacgg
     1201 ccgcgtcgcg gcagaccatg tcggcctcga cgcgggcctt cgggccgaag acggcgagtg
     1261 tggtgacgtc ctccccgtcc tccgcgaacg cgcgaacgag gaggtcgtcc agggcttcga
     1321 cgaggcgtcg atcggtcttc acgggaacag cattctagac ggcccggcgg gtgcgcgccg
     1381 tgggggcgtt cgcgtaggat gtcgcgccga tggaggactc gatgatggcc ggacccgtga
     1441 tcccggaacg ttaccgagcg gcgctcgggg acgacgaccc cttcctcgcg atgttcgagg
     1501 cgcctcaggc gctccggaag ctcctgaagg ggctcaccga gaagcagctc tcccgccgcc
     1561 ccgcgccgtc caggcgagcc agtgagcgag cgccgcgtat cgccgccgct ccctcgccgg
     1621 cagcaccagg gcggcctccc ggcggaggcg ctcgatctcg ctgcctgccc gggcgggatc
     1681 gcccgaggag ccgatgcgga gggcggccag gcgggtggcc cactcgggga gggggttccc
     1741 gacgagcgcc cctcccgcct cggggagga
//
LOCUS       DAOTEZ010000647         1765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_61016_length_1765_cov_6.459649, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000647 DAOTEZ010000000
VERSION     DAOTEZ010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61016_length_1765_cov_6.459649"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1734
                     /locus_tag="P5164_20385"
     CDS             <1..1734
                     /locus_tag="P5164_20385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HRY46301.1"
                     /translation="CFCYQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTVKGG
                     DFRARESNVHRLAEVSNDIIDQCVAQGVPFGREYGGTLGNRSFGGAQVSRTFYARGQT
                     GQQLLIGAYQALERQIGLGKVKMYPRTEMLDLIVIDGVARGIVVRDMVTGKISSHLAD
                     AVMLATGGYGNVFFLSTNAKGCNATAIWRAYRRGAYFGNPCYTQIHPTCIPVHGENQS
                     KLTLMSESLRNDGRIWVPLKKGETRKAADIPEAERDYYLERKYPSYGNLAPRDISSRA
                     AKQVCDEGRGVGPGGQGVYLDFAESIGRLGEGVIRERYGNLFEMYERITDENPYKVPM
                     RIYPAVHYTMGGLWVDYNLMSNVPGLFVLGEANFSDHGANRLGASALMQGLADGYFVI
                     PYTIGNYLAQGKPAKVDASHPAVKAAEAEVTERTKKLLSIKGKRTVDSLHKQLGLLMW
                     EKCGMGRNRAGLEEAIARIPEVREEFWKNVFVPGSGEDLNVSLEKAGRLADFLELGEL
                     MCRDALVREESCGGHFREEYQTPDGEALRNDEKFAHVAAWEWKGEGATPERHTEQLTF
                     ESVHLATRSYK"
BASE COUNT          322 a          617 c          592 g          234 t
ORIGIN      
        1 tgcttctgct accaggacag cccccgccgc gcgcactcca tcgccgcgca gggcggcatc
       61 aacgcggcga agaactacca gaacgacggc gacagcgtct accgcctctt ctacgacacg
      121 gtgaagggag gcgacttccg cgcgcgcgag tcgaacgtcc accgcctcgc cgaggtgagc
      181 aacgacatca tcgaccagtg cgtggcgcag ggcgtcccgt tcggccgcga gtacggcggc
      241 acgctcggga accgctcgtt cggaggcgcc caggtctcgc ggacgttcta cgcgcggggg
      301 cagacggggc agcagctcct catcggcgcc taccaggcgc tcgagcggca gatcggcctc
      361 ggcaaggtga agatgtaccc ccgcaccgag atgctcgacc tgatcgtgat cgacggggtc
      421 gcgcgcggca tcgtcgtccg cgacatggtc accgggaaga tctcgagcca cctcgccgac
      481 gccgtcatgc tcgcgacggg cggctacggg aacgtcttct tcctctcgac gaacgcgaag
      541 ggctgcaacg cgaccgccat ctggcgcgcc taccgccgcg gggcctactt cgggaacccc
      601 tgctacacgc agatccaccc cacctgcatc ccggtccacg gcgagaacca gagcaagctg
      661 acgctcatgt ccgagtcgct ccgcaacgac ggccggatct gggtgccgct gaagaagggg
      721 gagacgcgca aggcagccga catccccgag gccgagcgcg actactacct cgagcgcaag
      781 tacccgagct acgggaacct cgccccccgc gacatctcct cgcgcgcggc caagcaggtc
      841 tgcgacgagg ggcgcggcgt cggccccggc ggccagggcg tctacctcga cttcgccgag
      901 tcgatcggcc ggctcggcga gggcgtcatc cgcgagcggt acgggaacct cttcgagatg
      961 tacgagagga tcaccgacga gaacccgtac aaggtgccga tgcggatcta cccggcggtc
     1021 cactacacga tgggcggcct ctgggtcgac tacaacctca tgtcgaacgt ccccggcctc
     1081 ttcgtcctcg gcgaggcgaa cttctccgac cacggcgcga accgcctcgg cgcctcggcg
     1141 ctgatgcagg ggctcgccga cggctacttc gtcatcccgt acaccatcgg caactacctc
     1201 gcccagggca agcccgcgaa ggtcgacgcc tcccacccgg ccgtcaaggc ggccgaggcg
     1261 gaggtgacgg agcggacgaa gaagctcctc tcgatcaagg ggaagcggac ggtcgactcg
     1321 ctccacaagc agctcgggct cctcatgtgg gagaagtgcg ggatgggccg caaccgggcg
     1381 ggcctcgagg aggcgatcgc gaggatcccg gaggtccgcg aggagttctg gaagaacgtc
     1441 ttcgtccccg ggagcgggga ggacctgaac gtctcgctcg agaaggcggg gcggctcgcc
     1501 gacttcctcg agctcggcga gctcatgtgc cgcgacgccc tcgtccgcga ggagtcgtgc
     1561 ggcggccact tccgcgagga gtaccagact ccggacggcg aggcgctccg caacgacgag
     1621 aagttcgccc acgtcgccgc ctgggagtgg aagggcgagg gcgccacgcc cgagcgccac
     1681 acggagcagc tcacgttcga gtccgtccat ctcgccacga ggagctacaa gtaatgagcg
     1741 gcgacaagga catcgacatc aaggt
//
LOCUS       DAOTEZ010000648         1763 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_61168_length_1763_cov_3.814403, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000648 DAOTEZ010000000
VERSION     DAOTEZ010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61168_length_1763_cov_3.814403"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1652
                     /locus_tag="P5164_20390"
     CDS             <1..1652
                     /locus_tag="P5164_20390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008283284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRY46302.1"
                     /translation="RVILFFLVAYALTGLLVYLYSQSHRLSLVALGCSTIAVVWQLGL
                     LTLLGYGIDPLSILVPFLIFAIGVSHAVQMISANGAEIFDGADSITAARNSFRRLLVP
                     GFIALASDTIGFLTILLIKIRVIQEIAITASLGVAVIILTNLVLIPVILSYLHYPADY
                     NARLHRRAAHMAGFWRRLAQVSAPKPAAVIVAVAIALLGLGWWKGRDVKIGDLHRGVP
                     ELRADSRYNVDSAVITSKFSIGVDILTAIVETKPEGCVDHSVMTTIDDFAWHMENVEG
                     VQSTIALPAIAKMLNAGWNEGSPKWRVLSRNQSVLQQSVTYVPTTSGLLNSDCSVMPV
                     MIFTADHKADTIGKIVTEVKRFEAEHGHPDVKFRLATGNVGVMAATNEAVSAAQFPIL
                     AWVFGAVVVLCLLSFRSLRGTLCILVPLALVSLLAYALMAWLQIGLKVSTLPVVALGV
                     GVGVDYGIYIFARFRSLYRDGVPLTEAYRKTLAITGNGVLFTGITLGLGVATWIFSPL
                     KFQADMGLLLCFLFLMNMVGALVLLPALARGLLGDTHQERG"
     gene            complement(1672..>1763)
                     /locus_tag="P5164_20395"
     CDS             complement(1672..>1763)
                     /locus_tag="P5164_20395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HRY46303.1"
                     /translation="QQYLAKNPGGYCGLGGIGIACPAGLGVEG"
BASE COUNT          244 a          690 c          512 g          317 t
ORIGIN      
        1 gccgcgtcat cctcttcttc ctcgtcgcct acgccctcac gggcctcctc gtctacctct
       61 actcccagtc gcaccggctg tcgctcgtgg ccctcggctg ctccacgatc gccgtcgtct
      121 ggcagctcgg cctcctgacg ctcctcggct acggcatcga cccgctctcg atcctcgtcc
      181 ccttcctcat cttcgccatc ggcgtcagcc acgccgtgca gatgatcagc gccaacggcg
      241 ccgagatctt cgacggcgcc gactcgatca cggcagcccg gaacagcttc cggcggctcc
      301 tcgtgcccgg gttcatcgcg ctcgcgtcgg acaccatcgg cttcctgacg atcctcctca
      361 tcaagatccg ggtcatccag gagattgcga tcaccgccag cctcggcgtg gccgtcatca
      421 tcctgacgaa cctcgtcctg atccccgtca tcctgtcgta tctccactac cccgccgact
      481 acaacgcccg cctccaccgg cgcgccgcgc acatggcggg cttctggcgg cggctcgcgc
      541 aggtctccgc gccgaaaccc gccgccgtca tcgtcgccgt ggcgatcgcc cttctcggcc
      601 tcggctggtg gaaggggcgc gacgtgaaga tcggcgacct tcaccggggc gttcccgagc
      661 tgagggccga ctcgcgctac aacgtcgaca gcgcggtcat cacctcgaag ttctcgatcg
      721 gcgtcgacat cctcaccgcc atcgtcgaga cgaagcccga ggggtgcgtc gaccactccg
      781 tcatgacgac catcgacgac ttcgcgtggc acatggagaa cgtcgagggg gtccagtcga
      841 cgatcgccct ccccgcgatc gcgaagatgc tgaacgccgg ctggaacgag ggctccccga
      901 agtggcgcgt cctctcgcgc aaccagtccg tcctccagca gtcggtcacc tacgtcccga
      961 cgacctccgg cctcctgaac tccgactgca gcgtcatgcc ggtgatgatc ttcacggccg
     1021 accacaaggc cgacacgatc gggaagatcg tcacggaggt gaagcgattc gaggccgagc
     1081 acggccaccc cgacgtgaag ttccgcctcg cgaccggaaa cgtcggggtg atggccgcga
     1141 cgaacgaagc ggtctccgcg gcccagttcc cgatcctcgc ctgggtcttc ggggccgtcg
     1201 tcgtcctctg cctgctctcc ttccggtcgc tccggggaac cctctgcatc ctcgtcccgc
     1261 tcgccctcgt ctccctcctc gcctacgcgc tcatggcgtg gcttcagatc ggcctgaagg
     1321 tgtcgacgct ccccgtcgtc gccctcggcg tcggcgtcgg cgtcgactac ggcatctaca
     1381 tcttcgcccg gttccggtcg ctctaccggg acggcgtgcc gctcaccgag gcctaccgga
     1441 agacgctcgc gatcaccggc aacggcgtcc tcttcacggg aatcacgctc gggctcggcg
     1501 tcgcgacgtg gatcttctcg ccgctgaagt tccaggccga catggggctg ctcctctgct
     1561 tcctcttcct gatgaacatg gtcggcgccc tcgtcctgct gccggccctc gcgcgcggcc
     1621 tcctcggcga cacccaccag gagcgcggct gaggtcgcac cccgccgccg cctacccctc
     1681 gacgccgagc cccgccgggc aggcgattcc gatgccgccg aggccgcagt agccgcccgg
     1741 gttcttcgcg aggtactgct ggt
//
LOCUS       DAOTEZ010000649         1754 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_61714_length_1754_cov_4.194820, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000649 DAOTEZ010000000
VERSION     DAOTEZ010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61714_length_1754_cov_4.194820"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1754
                     /locus_tag="P5164_20400"
     CDS             <1..>1754
                     /locus_tag="P5164_20400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46304.1"
                     /translation="SYSLWQPTGSQQGSTVNALALDSTYDNAWKTLWTVSGPAAGHWE
                     LRVQSINTAATDNDYNAFAVRARASGRDLSIYVDGIAPFGTNRLAATDYVFFPYVVSG
                     CSCSSNDYDSDADALSYGYRLATRTGARSWGPFTPAANGAWVSGAASGFTTANDATDY
                     GIWSGSKSKPSNVANIYNYYVGAWNASAPPPAANPVASSVRLYAPTDAGAAPVKPYFE
                     QVTRYKSGMNPPASGFTTTMRVTAYLVNPTPYAITFSSTNVVRTAVPATRTTYGGNAS
                     ATQGTIVSQPAVGGTGTITWNPGTVAAGATVALAYDVVVTPTANGQTTSVTPEPNATV
                     PTTATFVDETGNTTQARATMTLGPLCQLSITAGTRPTLAVVASFSARTGADGTKVEWT
                     TSAADGTAGFVLERQRPGEAGFTPVHEGVLAGPVGALVGARWEYVDRDVTSGEAAAYR
                     LVEVEANGNRRPFGPWASEAADSAEPRSGEGPRYVVTPHPRDERAKPASGEPVSPQAR
                     RSELPGKPGMKIETEASGLYVVPVDAIASALGLTRPRATELVRAGRLELTNRGRAVAW
                     TPTKEGDGVVFWAEALED"
BASE COUNT          279 a          586 c          660 g          229 t
ORIGIN      
        1 tctcctactc gctctggcag ccgacagggt cgcagcaagg ctcgacggtc aacgccctcg
       61 ccctcgactc gacgtacgac aacgcgtgga agacgctctg gacggtttcc ggcccggccg
      121 cgggccactg ggagctgcga gtccagtcca tcaacacggc agccaccgac aacgactaca
      181 acgccttcgc cgtgcgtgcg cgcgcgagcg ggcgggacct gagcatctac gtcgacggca
      241 tcgcgccgtt cggcacgaac cggttggccg cgacagacta cgtcttcttc ccttacgtcg
      301 tctcgggctg ctcctgctcc tcgaacgact acgatagcga tgccgacgcg ctgtcgtatg
      361 gctaccgtct cgcgactcga accggggcga ggtcgtgggg gccgttcact cctgcggcga
      421 acggcgcctg ggtgagcggc gccgcctcgg gcttcacgac cgcaaacgac gcgaccgatt
      481 acggaatctg gagcgggagc aagtcgaagc cttcgaatgt cgcgaacatc tacaactact
      541 acgtcggggc ctggaacgcc tccgctccgc cgccggcagc gaacccggtc gcgagctcgg
      601 tgaggctcta cgcgccgacg gacgccgggg cggctccggt gaagccgtac ttcgagcagg
      661 tgacccggta caagtcggga atgaacccgc cggcgtccgg tttcacgacg acgatgcgcg
      721 tgacggccta cctcgtgaac ccgacgccgt acgcgatcac cttctcgtcg acgaacgtcg
      781 ttcggacggc cgtgccggcg acgcggacga cgtacggcgg gaacgcgagc gcgacgcagg
      841 ggacgatcgt ctcgcagccg gcggtcggcg ggacggggac gatcacctgg aaccccggca
      901 ccgtcgcggc cggagcgacc gtggcgctcg cgtacgacgt cgtcgtgacg ccgacggcga
      961 acgggcagac gacgagcgtg acgccggagc cgaacgcgac agttccgacg acggcgacgt
     1021 tcgtggacga gacagggaac accacccagg cgcgggccac gatgacgctc gggccgctct
     1081 gccagctctc gattactgcg gggacgaggc cgacgctcgc ggtcgtcgca tcgttctcgg
     1141 cccggacggg ggccgacggg acgaaggtgg agtggacgac gtcggcggcg gacgggacgg
     1201 ccgggttcgt cctcgagagg cagcggccgg gcgaagcggg tttcacgccg gttcacgagg
     1261 gggtcctcgc cgggccggtc ggggcgctcg tgggcgcccg ctgggagtac gtcgaccggg
     1321 acgtgacgag cggcgaggcg gcggcgtacc ggctggtgga ggtggaggcg aacgggaacc
     1381 ggcgaccctt cggaccgtgg gcgagcgagg cggcagattc cgcggagccg cgcagcggcg
     1441 aagggccgcg gtacgtcgtg acgcctcacc cgcgcgacga gcgggcgaag cctgcctccg
     1501 gtgagcccgt gtcgccgcag gcgcgacgct cggagctccc ggggaagccg gggatgaaga
     1561 tcgagaccga ggcatccggg ctgtacgtcg tccccgtgga cgcgatcgcg tcggcgctcg
     1621 gactcacgcg gccgagggcg acggagctcg tgcgcgcggg ccggctcgag ctgacgaacc
     1681 ggggccgtgc cgtggcgtgg accccgacga aggagggcga cggcgtcgtc ttctgggccg
     1741 aggctctcga ggac
//
LOCUS       DAOTEZ010000650         1750 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_61971_length_1750_cov_5.152212, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000650 DAOTEZ010000000
VERSION     DAOTEZ010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61971_length_1750_cov_5.152212"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="P5164_20405"
     CDS             <1..446
                     /locus_tag="P5164_20405"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY46305.1"
                     /translation="ITAELGTMAVTEQVDALRALGSSPIRKLVLPRVVALTLMLPILT
                     LIAIFHGMLGGLLIAVFELKIGASFYLSTTLQALSFNDLMHGLCKTPFFGLEIALIGC
                     YNGLSATGGADGVGRATTVAVVVASITILITDFFLSKLFIALPIG"
     gene            443..1192
                     /locus_tag="P5164_20410"
     CDS             443..1192
                     /locus_tag="P5164_20410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY46306.1"
                     /translation="MSDPVVELRAVSKAFGEKVVLDAVDLTVARGEVLVILGGSGSGK
                     SVTLRHMNGLTQPDAGEVFVEGVEVSRLAEEELGEVRRKVGMLFQMGALFDSMTVFEN
                     VAFALYEHTKMSGEEIAARVVEVLGFVDLGPDVLPLLPSSLSGGMKKRVSLARTIALK
                     PDVLLYDEPTTGLDPVTAMTINRLIVDLNSRLATTSVVVTHDIASALFVADRIAFLEK
                     GRFAFVGTPGEARSSDVAALRAFLAAEEAGA"
     gene            1209..>1750
                     /locus_tag="P5164_20415"
     CDS             1209..>1750
                     /locus_tag="P5164_20415"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRY46307.1"
                     /translation="MKADSRTRSRVGVIVFAAILLFAVMVVVVGGKTGFFLARSSYFA
                     RFPNSQGLNGGNQVRLAGVTVGAVQEVEVPKSPGEDLTVRFDIERRYRHLVRTDSRVE
                     IKTIGLLGDKYLEVTPGSPGTPLLEPGSEISAIRGSELDKILAGSGDLFENVNSVAES
                     LRTILGRVEKGEGFLGEMTSA"
BASE COUNT          246 a          651 c          564 g          289 t
ORIGIN      
        1 ggatcacggc cgagctcggg acgatggccg tcacggagca ggtcgacgcc ctccgggcgc
       61 tcggctcctc cccgatccgc aagctcgtcc tcccgcgcgt cgtcgcgctg acgctcatgc
      121 tcccgatcct cacgctcatc gcgatcttcc acgggatgct cggggggctc ctcatcgccg
      181 tcttcgagct gaagatcggg gcgagcttct acctctcgac gacgctccag gcgctctcgt
      241 tcaacgacct gatgcacggc ctctgcaaga cgcccttctt cggcctcgag atcgcgctca
      301 tcggctgcta caacggcctc tcggccaccg gcggcgccga cggcgtcggc cgcgccacga
      361 ccgtcgccgt cgtcgtcgcc tcgatcacga tcctgatcac cgacttcttc ctctccaagc
      421 tcttcatcgc cctgccgatc ggatgagcga ccccgtcgtc gagctccgcg ccgtctcgaa
      481 ggccttcgga gagaaggtcg tcctcgacgc cgtcgacctg acggttgcgc gaggggaggt
      541 cctcgtcatc ctcggcggct cgggcagcgg caagtcggtc acgctccggc acatgaacgg
      601 cctcacccag cccgacgcgg gggaggtctt cgtcgagggc gtcgaggtgt cgcgcctcgc
      661 cgaggaggag ctcggggagg tccgccggaa ggtcgggatg ctcttccaga tgggggcgct
      721 cttcgactcg atgacggtct tcgagaacgt cgccttcgcc ctctacgagc acacgaagat
      781 gagcggcgag gagatcgccg cccgggtcgt cgaggtcctc ggcttcgtcg acctcggccc
      841 cgacgtcctg ccgctgctgc cctcgtccct gtcgggcggg atgaagaagc gcgtctccct
      901 cgcccggacg atcgcgctga agcccgatgt cctcctctac gacgagccga cgaccgggct
      961 cgacccggtg acggcgatga cgatcaaccg cctcatcgtc gacctgaact cgcgcctcgc
     1021 gacgacctcc gtcgtcgtca cccacgacat cgcctcggcc ctcttcgtgg ccgaccggat
     1081 cgcgttcctc gagaaggggc gcttcgcctt cgtcgggacc cccggcgagg cgcggagctc
     1141 cgacgtcgcc gccctccgcg cgttcctcgc cgcggaggag gccggagcct gagggagacc
     1201 gccgcaccgt gaaagccgac agccgcaccc gttcccgcgt cggggtcatc gtcttcgccg
     1261 cgatcctcct cttcgccgtc atggtcgtcg tcgtcggcgg gaagaccggc ttcttcctcg
     1321 cccgctccag ctacttcgcg cgtttcccga actcgcaggg gctgaacggc ggtaaccagg
     1381 tccgcctcgc gggcgtcacc gtcggcgccg tgcaggaggt ggaggtcccg aagagccccg
     1441 gcgaggacct caccgtccgc ttcgacatcg agcgccgcta ccggcatctc gtgaggaccg
     1501 acagccgcgt ggagatcaag acgatcggcc tcctcggcga caagtacctc gaggtgacgc
     1561 ccggctcccc cgggacgccc ctcctcgagc cggggtcgga gatctccgcg atccggggga
     1621 gcgagctcga caagatcctc gccggctccg gggacctctt cgagaacgtg aactccgtcg
     1681 ccgagtcgct ccggacgatc ctcgggcgcg tcgagaaggg cgaggggttc ctcggcgaga
     1741 tgacgagcgc
//
LOCUS       DAOTEZ010000651         1748 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_62111_length_1748_cov_2.858830, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000651 DAOTEZ010000000
VERSION     DAOTEZ010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62111_length_1748_cov_2.858830"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="P5164_20420"
     CDS             <1..650
                     /locus_tag="P5164_20420"
                     /inference="COORDINATES: protein motif:HMM:NF036520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRY46308.1"
                     /translation="PRVRLPRRLAPRKSPTQDRARETVEAILEAAVDLFSSRGYARTS
                     TNHIARRAGVSVGSLYQYFPNKDAVLTSLLARHLAAVESAISACIPPLRDPAVPLRDG
                     IRRLLELLREVHEADPRLSRAIEQQVGQMPVVPEGLAGYKRDHVATLAEILSTRPDVR
                     PGNHALMAVLLAAVTETVSVVLVHAAPARFGRDEMLDEAVEALCRFVERPPGRLS"
     gene            655..>1748
                     /locus_tag="P5164_20425"
     CDS             655..>1748
                     /locus_tag="P5164_20425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46309.1"
                     /translation="MSRLLTVLGSAFLGVATVASGVPPGGSETKPAAPAPRPVDELFT
                     GTLAGLPAVAVLRVEAGGVRSGTYFYRRRGRDIALVSGGKKGLLLECAAEHAEVSDEP
                     CAAPTGVWSVTLAGDRVEGTWRASERAAAGKPISFVRTPIGPGENAFLRYEATRRALF
                     SFADVSRHERSGVAWRIVKEKLSGVTGPLLVSGPRPEVVAKVNADLEAGLRDDAASRL
                     APGEFEVRHTVELANERTFSVSTSTGWDSPGAAHPSNGFNAVTWDLATGEKVDWSRVV
                     RLVDARAPKLDLARKDLLAAITIRRILSGPGGDEEGGCLGAALESYGCSETTCEAASL
                     YPGAASGVDTWVLFPTEAGLAVAPGSYSEA"
BASE COUNT          237 a          648 c          618 g          245 t
ORIGIN      
        1 ctccccgcgt ccggctcccc aggaggctcg ccccgcggaa gagcccgacc caggaccggg
       61 cccgcgagac ggtggaggcg atcctcgagg cggcggtcga cctcttctcc tcgagggggt
      121 acgcgcggac gagcacgaac cacatcgccc ggcgggcggg cgtttcggtg gggtcgctct
      181 accagtactt cccgaacaag gatgccgtcc tgacgtctct cctggcgcgg cacctcgccg
      241 ccgtggaatc ggcgatcagc gcctgcattc ccccgcttcg ggatcccgcg gttcccctcc
      301 gggacgggat ccggcgcctc ctcgagctgc tgcgcgaggt gcacgaggcc gatccccggc
      361 tctctcgcgc catcgagcag caggtcggtc agatgccggt ggtccccgaa gggctggccg
      421 gctacaagcg ggaccacgtc gccacgctgg cggagatcct gtcgacccgg cccgacgtcc
      481 gcccgggcaa tcacgcgctg atggccgtac tcctcgccgc ggtcaccgaa accgtcagcg
      541 tcgtcctcgt ccacgcggcg ccggcccgct tcggccggga cgagatgctc gacgaggccg
      601 tcgaggcgct ctgccgcttt gtcgagcggc cgccgggccg gttatcgtga ccctatgagc
      661 aggctcctca cagtcctggg ttccgccttc ctgggcgtgg ccaccgtcgc gagcggggtg
      721 ccgcccggcg gatcggagac gaagcccgcc gcgccggccc ccaggccggt cgacgagctc
      781 ttcaccggaa ccctcgccgg cctccccgcc gttgcggtgc tgcgggtcga ggcgggcggc
      841 gtccggtcgg gcacctactt ctaccggcgg cgggggcggg acatcgccct cgtctccggc
      901 ggaaaaaagg gacttctcct cgagtgcgcg gccgagcacg ccgaggtgag cgacgagccc
      961 tgcgccgcgc cgaccggcgt ctggtccgtg acgctcgcgg gggaccgcgt cgaggggacg
     1021 tggcgcgcga gcgagcgagc ggcggcgggc aaaccgatct ccttcgtacg cacgccgatc
     1081 ggccccggcg agaacgcgtt cctccgttac gaggcgacac gccgagccct cttctccttc
     1141 gccgacgtct cgcgccacga gcgcagcggc gtcgcctgga ggatcgtgaa ggagaagctc
     1201 tcgggggtga ccgggccgct cctcgtctcc ggtccgcgcc cggaggtcgt cgcgaaggtg
     1261 aacgccgacc tcgaggcggg gctgcgcgac gacgctgcgt cgcgccttgc gcccggggaa
     1321 ttcgaggttc gccacaccgt cgagctcgcg aacgagagga ccttcagcgt cagcacgtcc
     1381 accggctggg actcacccgg ggccgctcac ccttcgaacg ggttcaacgc cgtcacctgg
     1441 gacctcgcta cgggggagaa ggtcgactgg agccgggtcg tccggctcgt cgacgccagg
     1501 gctccgaaac tggacctcgc gcggaaggac ctcctcgccg cgatcacgat ccggaggatc
     1561 ctctcgggac cgggaggaga cgaggaaggc ggatgcctcg gtgccgccct cgaaagctac
     1621 ggctgctccg agacgacctg cgaggcggcc tcgctctacc caggggccgc gtcgggcgtc
     1681 gacacctggg tcctcttccc gaccgaagcg ggcctcgccg tcgccccggg ctcctacagc
     1741 gaggccga
//
LOCUS       DAOTEZ010000652         1740 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_62614_length_1740_cov_5.648071, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000652 DAOTEZ010000000
VERSION     DAOTEZ010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62614_length_1740_cov_5.648071"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="P5164_20430"
     CDS             <1..721
                     /locus_tag="P5164_20430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HRY46310.1"
                     /translation="EAFNRHIVNAPGMHRLAELTRHEILPTPGAVLRGAELFAEVAGD
                     CLVFDVGGATTDVHSVTEGSADWHARLVEPEPRAKRTVEGDLGVFVNARNVVDLAGEP
                     DGEERLADLAALPVTDRQKALTRWLCERAVSIGARRHAGVVSDLYTPTGRKQVVKGKD
                     LTAVRWVVATGGALTKVEGAADCLRAVCTGAGKHLLPDPSATILVDAEYRFSALGTLA
                     HAYPDDVRATFRSWIGARSEP"
     gene            731..>1740
                     /locus_tag="P5164_20435"
     CDS             731..>1740
                     /locus_tag="P5164_20435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HRY46311.1"
                     /translation="MFIRTPRLEIYPDRIAQNARAVIALCHEHGAQVAAVTKVVCAHP
                     AVVNALVAGGADMVADSRIRNLQAMADTGIDRPLMLLRIPAPSRAADVVRCANVTLNS
                     NLGTIELLSEAAVLHGVTHQVIVMVDVGDLREGVWPDRAIEVVRGAARLRNIEVIGLG
                     CNLACYGGVIPTKENMTALVEVRNRCREASGLDLNVLSGGNSANLPLLVSGQMPWEIN
                     QFRIGEAIVLGRNVIDRSPWPGTRQDTFKMVAEVVEVERKPSMPVGERGQDAFGGTPE
                     FEDRGVRLRAICNLGRQDVVVDGIEPLMPGITILGGSSDHLILDVEDAADIGEQLGLS
                     AV"
BASE COUNT          260 a          636 c          606 g          238 t
ORIGIN      
        1 cgaggccttc aaccgccaca tcgtgaacgc gccggggatg catcgcctcg cggagctgac
       61 ccggcacgag atcctcccga cgccgggcgc cgtcctccgc ggcgccgagc tcttcgccga
      121 ggtcgcgggc gactgcctcg tcttcgacgt cggcggcgcg acgaccgacg tccactccgt
      181 gacggagggg agcgccgact ggcacgcccg tctcgtcgag ccggagccgc gcgcgaagcg
      241 gaccgtcgag ggggacctcg gcgtcttcgt gaacgcgcgg aacgtcgtcg acctcgcggg
      301 cgagccggac ggcgaggagc gcctcgccga cctcgccgct ctccccgtca ccgaccgcca
      361 gaaggcgctc acccgctggc tctgcgagcg tgccgtctcg atcggcgccc gccgccacgc
      421 gggggtcgtc tccgacctct acaccccgac cggccggaag caggtcgtca agggaaagga
      481 cctgaccgcc gtacgctggg tcgtcgcgac cggtggcgcc ctcacgaagg tggagggggc
      541 ggccgactgc cttcgcgccg tctgcaccgg cgcggggaaa cacctccttc ccgacccttc
      601 ggccacgatc ctcgtcgacg ccgagtaccg cttcagcgcc ctcggcaccc tcgcccacgc
      661 gtaccccgac gacgtccgcg cgacgttccg aagctggatc ggcgcgcgct cggagccgta
      721 gaggagcctc atgttcatcc ggacgccccg cctcgagatc taccccgacc ggatcgccca
      781 gaacgcccgg gccgtcatcg ctctctgcca cgagcacggg gcgcaggtgg cggcggtgac
      841 gaaggtcgtc tgcgcccacc cggcggtcgt caacgccctg gtggcgggcg gagccgacat
      901 ggtcgccgac agccgcatcc ggaacctcca ggcgatggcc gacaccggga tcgaccggcc
      961 cctcatgctc ctccgtatcc cggcgccgag ccgggccgcc gacgtcgtcc gctgcgcgaa
     1021 cgtcacgctc aacagcaacc tcgggacgat cgagctcctc tcggaagcgg ccgtcctcca
     1081 cggcgtcacg caccaggtga tcgtcatggt cgacgtcggc gacctgcgcg agggggtctg
     1141 gcccgaccgg gcgatcgagg tcgtccgcgg cgccgcgagg ctccggaaca tcgaggtgat
     1201 cggcctcggc tgcaacctcg cctgctacgg cggcgtcatc ccgacgaagg agaacatgac
     1261 ggccctcgtc gaggtgagaa accggtgccg ggaggcctcg gggctcgacc tgaacgtcct
     1321 ctccggcggc aacagcgcga acctcccgct cctcgtgagc gggcagatgc cgtgggagat
     1381 caaccagttc cggatcggcg aggcgatcgt cctcggccgg aacgtcatcg accggagccc
     1441 ctggccgggg acgcggcagg acacgttcaa gatggtcgcg gaggtcgtcg aggtggagcg
     1501 caagccgtcc atgccggtcg gggagcgcgg tcaggacgcg ttcggcggga cgccggagtt
     1561 cgaggatcgg ggcgtgcggc ttcgggccat ctgcaacctc ggccggcagg acgtcgtcgt
     1621 cgacggaatc gagcccctca tgcccggcat cacaattctc ggcggctcca gcgatcatct
     1681 gatcctcgac gtcgaggatg cggcggatat tggggagcag ttgggtctca gcgccgtgga
//
LOCUS       DAOTEZ010000653         1733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_63052_length_1733_cov_5.110250, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000653 DAOTEZ010000000
VERSION     DAOTEZ010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63052_length_1733_cov_5.110250"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="P5164_20440"
     CDS             complement(<1..194)
                     /locus_tag="P5164_20440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46312.1"
                     /translation="MKKQFGIPVLVALLAAPGAALAQSDAERIADLEKRVESLVKKEK
                     ERAAAEEKAKSAKGTTLRSV"
     gene            complement(231..995)
                     /locus_tag="P5164_20445"
     CDS             complement(231..995)
                     /locus_tag="P5164_20445"
                     /inference="COORDINATES: protein motif:HMM:NF012922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HRY46313.1"
                     /translation="MKKSPRAAFALALALAAAAFSTALAAQEVTPYASVLKGEADFLS
                     GYEPINLDGTINAVVEIPAGTTAKYDTNVNTGMIELEQKDGKPRFVQYLGYPCNYGHV
                     PRSVLLKSKGGDGDALDVCVLGMPAPTGSVVKARAVGILTLLDNGEVDDKLIAVAQTG
                     PFARVSSLADLDRRFPGVTTILQTWFTHYKGYGKDGKLQLTSPGFKGRAVAIKTIGDA
                     VLDFETSVVTEADKRPLDEKGNPYLYRWPGARNIGE"
     gene            complement(1039..>1733)
                     /locus_tag="P5164_20450"
     CDS             complement(1039..>1733)
                     /locus_tag="P5164_20450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46314.1"
                     /translation="DRNGKVLVCPYLQVATKEFPAILDASMAPYNYVIDAEGRVTIIP
                     EAAHPLGRTYPNGFFRPEDQSEKKPGTRENFGHVSSLAGGPGRISGEILYDKDTGTYT
                     VNNKSGRYSKHNRDRTPEQLANAAKLIRQVVDTAGVAWGPALYLLEYAPVDVREQLAK
                     SPELRYDDEKKKKRPHVVVLPGAPSALSTDAPIAVAAAPAAAPAAVDAPKAAPSAPEK
                     KKAKAAHNDDPS"
BASE COUNT          208 a          582 c          613 g          330 t
ORIGIN      
        1 ctcacggagc ggagcgtcgt ccccttcgcg gacttcgcct tctcctcggc ggccgcccgc
       61 tccttctcct tcttcacgag gctctccacc ctcttctcga ggtcggcgat cctctcggcg
      121 tccgactggg ccagtgccgc ccccggagcc gcgagcaggg ccaccaggac cgggatcccg
      181 aactgcttct tcatgtcgta ctcctgcgtc tcttcgcgcc gctgccgcgg tcactccccg
      241 atgttcctgg cgcccggcca gcggtagagg taagggttgc ccttctcgtc gagggggcgc
      301 ttgtcggcct cggtcacgac ggaggtctcg aagtcgagga ccgcgtcgcc gatcgtcttg
      361 atcgcgacgg cccgtccctt gaagcccggc gacgtgagct ggagcttccc gtccttgccg
      421 tagcccttgt agtgcgtgaa ccaggtctgg aggatcgtcg tcacgcccgg gaagcgcctg
      481 tcgaggtcgg cgaggctgct gaccctcgcg aaaggcccgg tctgggcgac cgcgatcagc
      541 ttgtcgtcga cctcgccgtt gtcgaggagg gtcaggatgc cgacggcgcg cgccttcacg
      601 acggagcccg tcggagcggg catcccgagg acgcagacgt cgagcgcgtc gccgtcgccc
      661 cccttgctct tcaggaggac cgagcgcggg acgtggccgt agttgcacgg gtagccgagg
      721 tactgcacga agcgcggctt cccgtccttc tgctcgagct cgatcatccc ggtgttcacg
      781 ttcgtgtcgt acttggccgt cgtcccggcg gggatctcga cgacggcgtt gatcgtcccg
      841 tcgaggttga tcggctcgta gccgctcagg aagtcggcct cccccttgag gaccgacgcg
      901 tagggggtca cttcctgggc tgcgagggcc gtcgagaacg cggccgccgc gagggcgagc
      961 gccagcgcga aagcggcgcg cggggacttc ttcatggtct ctcggctcct tgtcctgtct
     1021 tcactcatcg cccggcggtc aggacgggtc atcgttgtgc gcggccttgg ccttcttctt
     1081 ctccggggcg gaaggggcgg ccttcggcgc atcgacggcg gcgggggccg cggccggagc
     1141 ggcggccacg gcgatcgggg cgtccgtgct cagcgccgag ggggcgcccg gcaggacgac
     1201 gacgtgagga cgcttcttct tcttctcgtc gtcgtaccgg agctccgggc tcttcgccag
     1261 ctgctcccgg acgtcgaccg gcgcgtactc gaggaggtag agggccggcc cccaggcgac
     1321 gccggccgtg tcgacgacct gccggatcag cttcgcggcg ttcgccagct gctccggagt
     1381 ccggtccctg ttgtgcttgc tgtagcggcc cgacttgttg ttgaccgtat aggttccggt
     1441 gtccttgtcg tagaggatct cgccgctgat ccggccgggg ccgccggcga gggaggagac
     1501 gtggccgaag ttttcgcggg tcccgggctt cttctccgac tggtcctccg gacggaagaa
     1561 gccgttcggg taggtgcggc cgagcggatg ggccgcctcg gggatgatcg tgacccgccc
     1621 ttcggcgtcg atgacgtagt tgtaaggggc catgctcgcg tcgaggatgg ccgggaactc
     1681 cttcgtggcg acctggagat acgggcagac gaggaccttc ccgttccggt ccg
//
LOCUS       DAOTEZ010000654         1714 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_64289_length_1714_cov_5.402049, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000654 DAOTEZ010000000
VERSION     DAOTEZ010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64289_length_1714_cov_5.402049"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="P5164_20455"
     CDS             <1..848
                     /locus_tag="P5164_20455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HRY46315.1"
                     /translation="GKTELYRDLLRHLDRSGDRFERARGERGMVMAVFTMPGYDVVFK
                     VIRDRFGPAKSVTREQVRERYQFVFEHDRAGRLVDVQEYELLEFAKERFEPSLLEELL
                     GECSENVTLRGGDVVIRHLYTERRVAPLNLFLREAPEERAREAVLDFGRALRDLAATN
                     VFPGDLLVKNFGVTRHGRVVFYDYDELCAVTDCDFRELPEQDEDGGGDGPSFYVGPRD
                     VFPEEFLTFMGFPAPLRRLFAESHGDLLAPRFWNEMKRRHFAGEVLDLHPYPPERRLR
                     GGADA"
     gene            937..>1714
                     /locus_tag="P5164_20460"
     CDS             937..>1714
                     /locus_tag="P5164_20460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRY46316.1"
                     /translation="MPAPPNGEKIRIVDGKLVVPENPVVPFIEGDGTGPDIWRASVRV
                     LDAAVAKAYGGKRKIAWMEVLAGQKSYDNLGTWLPDETVAAFQEYLVGIKGPLTTPIG
                     GGIRSLNVALRQLLDLYVCLRPVRWYEGVPSPVKRPEKVDMVIFRENTEDIYAGIEFE
                     SGSEDAKKALEFLKAGWPAMYKKIRFPESAAIGFKPVSREGSERLVRAAIRYAIDNKR
                     KSVTIVHKGNIMKFTEGAFRNWGYELAEREFAGETYTWDQW"
BASE COUNT          280 a          587 c          587 g          260 t
ORIGIN      
        1 acggaaagac cgagctctac cgcgacctcc tccggcacct cgaccggtcc ggcgaccgtt
       61 tcgagcgggc gcgcggcgaa cgcgggatgg tgatggccgt cttcacgatg ccgggctacg
      121 acgtcgtctt caaggtgatc cgcgaccggt tcgggccggc caagagcgtc acccgcgagc
      181 aggtgaggga gcgctaccag ttcgtcttcg agcacgaccg ggccggacgg ctcgtcgacg
      241 tccaggagta cgagctcctc gagttcgcga aggagcgctt cgagccgtcc ctcctcgagg
      301 agctgctcgg cgagtgctcc gagaacgtga cgcttcgggg cggcgacgtc gtcatccggc
      361 acctctacac cgagcgccgg gtcgcgccgc tgaacctctt cctccgcgag gcgcccgagg
      421 agagggcccg ggaggccgtc ctcgacttcg gacgggccct ccgggacctc gccgcgacga
      481 acgtcttccc gggggacctc ctcgtgaaga acttcggcgt gacgcgccac ggccgggtcg
      541 tcttctacga ctacgacgag ctctgcgccg tgaccgactg cgatttccgc gagctcccgg
      601 agcaggacga ggacggcggc ggagacgggc cgtcgttcta cgtcggacca cgagacgtgt
      661 tccccgagga gttcctgacc ttcatgggct tcccggcgcc tctccggcgc cttttcgccg
      721 agtcgcacgg cgacctcctc gccccccgct tctggaacga gatgaagcgc cggcacttcg
      781 cgggggaggt cctcgacctc catccctacc cgcccgagcg ccgcctgcgg ggcggagcgg
      841 acgcgtgagg ggagcggctc ctcgcgtccg gtttgtcgcg tttcgagagc gccgctagaa
      901 tccgcccccc ggaggtacgt gatgtccgac acgaagatgc ccgccccgcc gaacggcgag
      961 aagatccgga tcgtcgacgg caagctcgtc gttcccgaga accccgtcgt tcccttcatc
     1021 gagggcgacg gcacgggacc cgacatctgg cgcgcctccg tccgcgtcct cgacgccgcg
     1081 gtcgcgaagg cctacggcgg gaagcggaag atcgcctgga tggaggtcct cgcgggccag
     1141 aagtcgtacg acaacctcgg gacctggctc cccgacgaga cggtggcggc gttccaggag
     1201 tacctcgtcg gcatcaaggg ccccctgacg acgccgatcg gcggcggcat ccggtccctg
     1261 aacgtcgcgc ttcggcagct cctcgacctc tacgtctgcc tccgccccgt ccgctggtac
     1321 gagggggtcc cctccccggt gaagcgtccc gagaaggtcg acatggtgat cttccgggag
     1381 aacacggaag acatctacgc cggcatcgag ttcgagtccg ggagcgagga cgcgaagaag
     1441 gcgctcgagt tcctgaaggc gggctggccg gcgatgtaca agaagatccg cttcccggag
     1501 tcggcggcaa tcgggttcaa gccggtctcc agggaaggga gcgagcggct cgtccgcgcc
     1561 gcgatccgct acgcgatcga caacaagcgt aagagcgtga cgatcgtcca caagggcaac
     1621 atcatgaagt tcacggaggg cgccttccgg aactggggtt acgagctcgc cgagcgggag
     1681 ttcgcgggcg agacctacac ctgggaccag tggg
//
LOCUS       DAOTEZ010000655         1708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_64700_length_1708_cov_7.078645, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000655 DAOTEZ010000000
VERSION     DAOTEZ010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64700_length_1708_cov_7.078645"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="P5164_20465"
     CDS             <1..238
                     /locus_tag="P5164_20465"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114726.1"
                     /note="heat shock protein involved in degradation of
                     misfolded proteins; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HRY46317.1"
                     /translation="LVVADREKTLLLSGTGDVISPDEGILAVGSGGPYATAAARALFR
                     HTDLGAEEIVRASLKIASEIDIYTNDSVSVETLP"
     gene            235..1593
                     /gene="hslU"
                     /locus_tag="P5164_20470"
     CDS             235..1593
                     /gene="hslU"
                     /locus_tag="P5164_20470"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HRY46318.1"
                     /translation="MTRPASPRELHLRDLSPRRIVAELDRHVVGQGKAKKAVAVALRD
                     RWRRQQLAPEVAREITPKNILMVGPTGVGKTEIARRIARLVDAPFVKVEASKFTEVGY
                     VGRDVESIARDLVEAAIRLVRDERRERVVEKAKAAAEEKLVELLSAQVDPAGFVPGPD
                     AKERLRRELRDGKHEAREVEVEVIDERTPQISVMTPQGVEEMGIDLPGMLPNLFGAPK
                     RKKARLPVSDARAILVDREADVLSDEATVPQEAIRRAEETGIVFLDEIDKIAGREGKG
                     GGPDVSREGVQRDLLPLVEGTTVKTKHGNVKTDHVLFVAAGAFHVARPSDLIPELQGR
                     FPIRVELDALTEADFVRILTEPEHSLPKQAQALLGTEGIDLVFAEDGLAEIAKAAAEA
                     NRSLENIGARRLHTILERVLSEASFAGPDAAGARVVVDRTAVARSLADLVASRDLSRF
                     VL"
BASE COUNT          249 a          637 c          606 g          216 t
ORIGIN      
        1 cctcgtcgtc gccgaccggg agaagacgct cctcctctcc gggacgggcg acgtcatctc
       61 gcccgacgag gggatcctcg ccgtcggctc gggcggcccc tacgccaccg ccgcggcgcg
      121 cgccctcttt cgtcacacgg acctgggggc cgaggagatc gtccgcgcct cgctgaagat
      181 cgcctccgag atcgacatct acacgaacga ctccgtctcc gtggagacgc tgccgtgacg
      241 cgtcccgcct cgccgcgcga gctccacctg cgcgacctct ccccgcggag gatcgtggcc
      301 gagctggacc ggcacgtcgt cggccagggg aaggcgaaga aggccgtggc cgtcgcgctg
      361 cgcgaccgct ggcggcgcca gcagctcgcc cccgaggtcg cccgcgagat cacgccgaag
      421 aacatcctca tggtcggccc gacgggcgtc ggcaagaccg agatcgcgcg ccggatcgcg
      481 cgcctcgtcg acgcgccgtt cgtgaaggtg gaggcgtcga agttcaccga ggtcggctac
      541 gtcggccggg acgtcgagtc gatcgctcgc gacctcgtcg aggcggcgat ccgcctcgtg
      601 cgggacgagc ggcgcgagcg cgtcgtcgag aaggcgaagg ccgcggccga ggagaagctc
      661 gtggagctcc tctccgccca ggtcgacccg gcgggcttcg tccccggccc cgacgcgaag
      721 gagcggctcc gccgcgagct gcgcgacggc aagcacgagg cgcgcgaggt ggaggtcgag
      781 gtcatcgacg agcggacgcc gcagatctcc gtcatgacgc cgcagggggt cgaggagatg
      841 gggatcgacc tccccgggat gctcccgaac ctcttcggcg ccccgaagcg gaagaaggcg
      901 cggctccccg tttccgacgc gcgagcgatc ctcgtcgacc gcgaggcgga cgtcctctcc
      961 gacgaggcga cggtcccgca ggaagcgatc cggcgcgcgg aggagaccgg gatcgtcttc
     1021 ctcgacgaga tcgacaagat cgcggggcgc gaggggaagg ggggcggccc cgacgtctcg
     1081 cgcgaaggag tccagcgcga cctcctccct ctcgtcgagg ggacgaccgt caagacgaag
     1141 cacgggaacg tgaagaccga ccacgtcctc ttcgtagccg ccggtgcgtt ccacgtcgcc
     1201 cggccgtccg acctgatccc ggagctgcag gggcgcttcc cgatccgcgt cgagctcgac
     1261 gccctgaccg aggcggactt cgtgaggatc ctgaccgagc cggagcactc ccttccgaag
     1321 caggcgcagg cgctcctcgg gaccgagggg atcgacctcg tcttcgccga ggacggcctc
     1381 gccgagatcg cgaaggccgc agccgaggcg aaccggagcc tcgagaacat cggcgcgcgg
     1441 cgcctccaca cgatcctcga gagggtcctc tccgaggcga gcttcgccgg ccccgacgcc
     1501 gccggcgcgc gcgtcgtcgt cgaccggacc gccgtcgcgc gctctctcgc ggacctcgtc
     1561 gcgagccgcg acctctcgcg cttcgtcctg tgagccgcag ccggacggtc ctgcttctcc
     1621 tcgccgcggc gctcgccgcc ggctgcgggc ggaagatcgc cccggaagcg cccctgcagg
     1681 tgatccccgc gcgccccgag ccgctccg
//
LOCUS       DAOTEZ010000656         1702 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_65098_length_1702_cov_4.971463, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000656 DAOTEZ010000000
VERSION     DAOTEZ010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65098_length_1702_cov_4.971463"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="P5164_20475"
     CDS             complement(<1..913)
                     /locus_tag="P5164_20475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46319.1"
                     /translation="MSGRQLGGHDTAEYLIHAQQTASNMRGGVFLPAWASDLNWGFGG
                     PGLLFYPPLANTLHALLPLAGLRLPTGLGGLALVAMLLSGLAVRGWLRAAGFGAGSHW
                     AAVLYVAAPYRLVDLYERTALAENLAFVFAPLVLWIAAEEKLAPSRRVALGALAVAAL
                     ALTNLPLFVLFGLVLGPLVFVLPSTRRSIRTLLLAAPLGLAVAAFSLGPQALASNWLR
                     TELWFGDAGAGAYRTSTNTLFNPAAMDSDFNTRVSMALVATVLLGLAAFVLASNQQRG
                     KDDARAWFLVIVVGFVVIGESLRSLSRQ"
     gene            complement(1151..>1702)
                     /locus_tag="P5164_20480"
     CDS             complement(1151..>1702)
                     /locus_tag="P5164_20480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRY46320.1"
                     /translation="NAEQANSAKVAGNKELTDIVSALGCGSGADFDLDKLRYGKVFLL
                     MDADADGHHISTLLLTFFWRHLPKLVTEGHVYLAQPPLYRIDAGKETYWALDDAERDA
                     VFAKLPKNVKPELMRFKGLGEMMPEQLKATTLDPRRRRALKVTVPDVLEAERTLSDLM
                     GKDAGARYRFITERAREADALDV"
BASE COUNT          298 a          554 c          617 g          233 t
ORIGIN      
        1 gctggcgcga caacgaacgg agagactccc cgatgacgac gaaaccgacg acgatgacga
       61 ggaaccaggc ccgcgcatcg tccttcccgc gctgctggtt cgatgcgagc acgaaagccg
      121 caaggccgag aaggaccgtc gcgacgagcg ccatcgagac ccgagtgttg aagtccgagt
      181 ccatggcggc gggattgaac agcgtattcg tcgaggtccg gtaggcgccc gctccggcgt
      241 cgccgaacca gagctccgtt cgcagccagt tcgaggcgag cgcctggggg ccgagcgaga
      301 aggccgcgac cgccaggccc aggggcgcgg cgaggagaag cgtacggatc gaccttcgcg
      361 tagagggaag cacgaagacg agcgggccga ggacgagccc aaagaggacg aaaagcggga
      421 ggttcgtcag agcgagcgca gccacggcca acgccccgag cgcgacgcga cggcttgggg
      481 caagcttctc ctcggcggcg atccagagga cgagcggcgc gaagacgaac gcaaggttct
      541 cggcgagggc ggtcctctcg tagaggtcga cgagccggta aggcgccgcg acgtaaagga
      601 ccgccgccca gtgcgacccg gcgccgaacc ctgcggcccg cagccatccc cgaacggcga
      661 gtccggacag gagcatcgcc acgagggcga gtcctccgag cccggtcggg aggcggagac
      721 cggcgagagg aaggagcgcg tggagcgtgt ttgcgagggg ggggtaaaaa aggaggccgg
      781 ggccgccaaa accccagttg aggtcactgg cccacgccgg caggaagacg ccgcccctca
      841 tgttcgaggc cgtctgctgg gcgtgaatga ggtattcggc ggtgtcgtgc ccgcccagct
      901 ggcgtccgga caagagcgga accccggcga ggagcacggc aaccacgaga gcggtgatcc
      961 cgacgaaggc ctcggatcgg gttcggcgcg gtgtctcgat tctgatgacg atgcgtgacc
     1021 ctcgaggaca ctgtcaaggg attccccgga gcctcgcggc cgggcgggcc gtcagacgtc
     1081 caggctgtcc accgctcgcc gggggggtct ggcttcgcgc gcccccccgg accccccacc
     1141 cttcttcagc tcacacgtcc agcgcatccg cttcccgggc ccgttccgtg atgaaccggt
     1201 agcgggcgcc ggcatccttg cccatgaggt cggagagggt ccgctcggcc tcgaggacgt
     1261 cggggacggt caccttgagc gcccgtcggc ggcgcgggtc gagggtcgtc gccttgagct
     1321 gctccggcat catctcgccg agccccttga atcgcatcag ctcgggcttc acgttcttcg
     1381 ggagcttcgc gaagacggcg tcccgctcgg cgtcgtcgag cgcccagtac gtctccttcc
     1441 ccgcgtcgat cctgtagagc ggcggctggg cgaggtagac gtgcccctcg gtgacgagct
     1501 tcggcaggtg cctccagaag aacgtcagga gaagggtcga gatgtggtgg ccgtcggcgt
     1561 cggcgtccat caggaggaag accttgccat agcgcagctt gtcgaggtcg aagtcggcgc
     1621 cgctcccgca gccgagcgcc gagacgatgt cggtcagctc cttgttcccg gcgaccttcg
     1681 ccgagttcgc ctgctcggcg tt
//
LOCUS       DAOTEZ010000657         1700 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_65218_length_1700_cov_5.911854, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000657 DAOTEZ010000000
VERSION     DAOTEZ010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65218_length_1700_cov_5.911854"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..735
                     /locus_tag="P5164_20485"
     CDS             52..735
                     /locus_tag="P5164_20485"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HRY46321.1"
                     /translation="MTRSHTFAPAAALLLAGLAAFAALAAPALPARAAESAPPEAAAL
                     LRSIVATYTGKPAFRMTFVQSYAPSGFPDASPETGKLTIQAPHFLRFDYDGPEGKLYT
                     FDGRTAHQYVAADRQVMRKTLTAAEKERLPLLFLKPPAEVLERFAPAVKRDRNGLLEL
                     TLTPRGESELKSVSALAAPSGELKRLVVLDAEENRTTFTFTKMEPAKKRPLSDFDLVP
                     PKGTRVVGD"
     gene            751..1248
                     /locus_tag="P5164_20490"
     CDS             751..1248
                     /locus_tag="P5164_20490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HRY46322.1"
                     /translation="MADPSFDVVSEVSMPEVVNAVAQAQKEVVQRFDLKGTAAGIELK
                     EKEKQIVLTANDDFGLKAVNDVLQNKLIKRNVALKSLVYGTVEPAAKGTVRQVVTIQQ
                     GIETEKAKEIVKAIKDAKLKVQAAIQGEQVRVTGKKRDDLQAVIALLKGKDFGLPLQF
                     TNYRN"
     gene            1367..>1700
                     /locus_tag="P5164_20495"
     CDS             1367..>1700
                     /locus_tag="P5164_20495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRY46323.1"
                     /translation="MNSTSLTDRLLLANLPDEERANGQRLVRALSLVETKLRGVEEEL
                     ERRTRSEVPTISLIGEYLVDGGGKRIRPALLLLASRLLGYEGEVDVKYAAVLELIHTA
                     TLVHDDIID"
BASE COUNT          293 a          605 c          568 g          234 t
ORIGIN      
        1 cgctcgtctt cagcggccgg cgccacttcc ggcactgagg gacagagagg aatgacccgt
       61 tcccacacct tcgccccggc cgcggcgctc ctcctcgcgg gcctcgccgc cttcgcggcc
      121 cttgctgcgc ccgcgctccc cgcccgcgcg gccgagtcgg cgcctcccga ggccgccgcg
      181 ctcctccgct cgatcgtcgc cacgtacacg ggcaagccgg cgttccgaat gacgttcgtc
      241 cagagctacg cgccgtcggg cttccccgac gcgtcgccgg agaccgggaa gctgacgatc
      301 caggcgcccc acttcctccg cttcgactac gacgggccgg aggggaagct ctacacgttc
      361 gacggccgga cggcccacca gtacgtcgcc gccgaccggc aggtgatgcg caagacgctc
      421 acggccgcgg agaaggagcg gctcccgctc ctcttcctga agcccccggc ggaggtcctc
      481 gagcgcttcg cgccggccgt gaagcgggac aggaacggcc tcctcgagct gacgctcacg
      541 ccgcgcggcg agtcggagct gaagtccgtg tcggcgctcg cggcgccctc cggcgagctg
      601 aagcgcctcg tcgtcctcga cgccgaggag aaccggacga cgttcacgtt cacgaagatg
      661 gaacccgcga agaagcgccc gctctccgac ttcgacctcg tcccgccgaa ggggacgcgc
      721 gtcgtcgggg actgagcgaa ggagagagag atggccgacc ccagcttcga cgtcgtttcc
      781 gaggtctcga tgcccgaggt cgtcaacgcg gtcgctcagg cccagaagga ggtcgtccag
      841 cggttcgacc tcaaggggac cgctgccggg atcgagctga aggagaagga gaagcagatc
      901 gtcctcaccg cgaacgacga cttcggcctg aaggccgtca acgacgtcct ccagaacaag
      961 ctcatcaagc ggaacgtcgc cctcaagtcg ctcgtctacg gaacggtgga gccggcggcg
     1021 aaggggacgg tccggcaggt cgtcacgatc cagcagggga tcgagacgga gaaggcgaag
     1081 gagatcgtca aggcgatcaa ggacgccaag ctgaaggtcc aggcggcgat ccagggggag
     1141 caggtccggg tcaccggcaa gaagcgggac gacctgcagg ccgtcatcgc cctcctgaag
     1201 gggaaagact tcgggctgcc tctgcagttc acgaactacc ggaactgatc tggatcgggg
     1261 aggctcgcca cgagcctccc cgcggcggct ccgccccctt cgggcatccc ggcgcggttc
     1321 ccgggcgtat cgacggaatg gacggtaata tccacgccgg aatcggatga actcgacctc
     1381 cctgacggac cgtcttctcc tcgcgaatct cccggatgag gagagggcca acgggcagcg
     1441 cctcgtccgg gcgctctccc tggtcgaaac gaagctccgc ggcgtggagg aggagctcga
     1501 gcgccggacc cgctccgagg tcccgacgat ctcgctcatc ggcgagtacc tcgtcgacgg
     1561 cggcggcaag cggatccgcc ccgccctcct gctcctggcg tcccgcctcc tcggctacga
     1621 gggggaggtc gacgtcaagt acgccgccgt cctggagctg atccacacgg cgacgctcgt
     1681 ccacgacgac atcatcgacg
//
LOCUS       DAOTEZ010000658         1694 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_65645_length_1694_cov_5.352654, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000658 DAOTEZ010000000
VERSION     DAOTEZ010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65645_length_1694_cov_5.352654"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..451)
                     /locus_tag="P5164_20500"
     CDS             complement(20..451)
                     /locus_tag="P5164_20500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46324.1"
                     /translation="MKQNHFRLFAILIGLLVCGAAGYGVYHFSQVPADGHFVDPAFAP
                     PLAESASRVVVYVAGEPILKAIEAQKVILKRKEGDAPLAATEIVASLNERDGARLAKV
                     PLMLGLAALAGGAFVLFLIGLFTPLISALKPPMIVDLHLEE"
     gene            complement(695..1444)
                     /locus_tag="P5164_20505"
     CDS             complement(695..1444)
                     /locus_tag="P5164_20505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46325.1"
                     /translation="MTRRLLGFAPVLIAALGLADRPAAAEGFRLDLETGAAWQLRNDF
                     AVPGAGGTRLSIDDADRGPFLAARATLWADLGERSSVRLVAAPFRAKANLTPETPIDF
                     DGATFAAGEPLDATWVFDSWRLSYVWRFRSSGPVTFRAGLTAKIRDARIELTQGDFSR
                     AKTNTGFVPLLYGGVRWQVSPRLALDLEADGAAARQGRAVDVAARLEANVSPDVDLFL
                     GARTLEGGVDGDEVYSFAWLAYGVGGVSLRF"
     gene            complement(1496..>1694)
                     /locus_tag="P5164_20510"
     CDS             complement(1496..>1694)
                     /locus_tag="P5164_20510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46326.1"
                     /translation="GRVEGFYFVDPEGTAWATDREERGGLVVVSARAETPGPEWGLRV
                     RLKTPGTLRRYRFALKDVPLP"
BASE COUNT          249 a          611 c          622 g          212 t
ORIGIN      
        1 cgccgcgccc gcgccggtgt cactcctcga ggtggaggtc cacgatcatc ggcggcttca
       61 gcgcgctgat gagcggcgtg aagagcccga tgaggaagag gacgaacgcg cccccggcca
      121 gcgccgcgag gcccagcatc agagggacct tcgcgaggcg cgctccgtcg cgctcgttga
      181 ggctcgccac gatctccgtg gcggcgagcg gcgcatcgcc ctccttcctc ttcaggatca
      241 ccttctgcgc ctcgatcgcc ttcaggatcg gctctcccgc gacgtagacc acgacgcggg
      301 aggcggactc ggcgagcggc ggcgcgaacg ccggatcgac gaagtggccg tcggccggga
      361 cctgcgagaa gtggtagacg ccgtagccgg ccgccccgca gacgagcaga ccgatcagga
      421 tcgcgaacag gcgaaagtgg ttctgtttca tttcgctgcc tctccggagg tccggctcgg
      481 cggaaggaag gagggccgct cccccggggg ccgcggcgag ccggcgaggc cggaagccga
      541 ggggggatgc ggaggcgccg gaggaggaga aggccggttc cggcggcgag ccgatcatag
      601 tcctccgatc ggctccccgc tcccgatccc tcgttcgggg cgcgggtcga tcgggcccga
      661 gatctccgat cgacgcccga gcccgggcgc ccgctcagaa ccggagggag acgcccccga
      721 caccgtaggc gagccaggcg aagctgtaga cctcgtctcc gtcgacgccc ccctcgagcg
      781 tccgtgcccc gaggaagagg tccacgtccg gcgagacgtt cgcctcgagc cgggccgcga
      841 cgtccaccgc gcgtccctgg cgcgcagcgg cgccgtcggc ctcgaggtcg agagcgagac
      901 gcggggatac ctgccagcgg acgcccccgt agaggagcgg cacgaaaccc gtgttcgtct
      961 tggccctcga gaagtctccc tgcgtcagct cgatccgcgc gtcgcggatc ttggctgtca
     1021 gcccggctcg gaacgtcacc ggtcccgacg agcggaaacg ccagacgtag gagagccgcc
     1081 acgagtcgaa cacccaggtc gcgtcgagcg gctcccccgc ggcgaaggtc gctccgtcga
     1141 aatcgatcgg ggtctcgggc gtgaggttcg ccttcgcgcg gaagggggcg gcgacgagcc
     1201 gcaccgagct ccgctcgccg aggtcggccc acagggtcgc ccgggcggcg aggaaggggc
     1261 cgcggtcggc gtcgtcgatc gagaggcgcg tcccgcccgc tccggggacg gcgaagtcgt
     1321 tccggagctg ccaggcggct cccgtctcga ggtcgagccg gaacccctcc gccgcggcgg
     1381 ggcggtcggc cagacccagg gccgcgatga ggacgggggc gaatccgagg aggcgtctcg
     1441 tcacgccccg attccgcgac cgcggcttgt ccggatttcc ccgcgcgccc cggtctcagg
     1501 ggagcgggac gtccttcagg gcgaaccggt aacggcggag cgtgcccggc gtcttcagcc
     1561 ggacccggag cccccattcc ggcccggggg tctcggcccg cgccgaaacg acgacgagcc
     1621 cgcccctctc ctcgcggtcg gtcgcccagg ccgtcccctc gggatcgacg aagtagaagc
     1681 cctcgacgcg tccg
//
LOCUS       DAOTEZ010000659         1688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_66010_length_1688_cov_6.083895, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000659 DAOTEZ010000000
VERSION     DAOTEZ010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66010_length_1688_cov_6.083895"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="P5164_20515"
     CDS             complement(<1..1060)
                     /locus_tag="P5164_20515"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRY46327.1"
                     /translation="MLDSSTPIQWLGADILTTLVFLPSLGALLLLLLPNGAKNAIRAV
                     ALLASGATFALSVLVLQRFNPAIAGLEAFRPLLVDRPWIPRFGIRYLLGVDGLSLILV
                     VLTTLLTLCVLVFSFGQTIPKLRGYVAAFLVLETGMIGSLVALDAVLFYVFWEVMLVP
                     MYFIIGIWGGKRRIYATMKFVLFTLAGSLLMFVAILFASGVHAKATGILTFSLLEWIP
                     AASSGAWSLTASEQALLFWAFALAFLVKVPLWPLHTWLPDAHVEAPTGGSIILAGVLL
                     KLGTYGLLRFAIPLFPGAAARYTPLIAVLALIGVVYGAWVAAAQKDMKKLVAYSSVSH
                     LALVVLGIFAGNVTAVSGA"
     gene            complement(1067..>1688)
                     /locus_tag="P5164_20520"
     CDS             complement(1067..>1688)
                     /locus_tag="P5164_20520"
                     /inference="COORDINATES: protein motif:HMM:NF013198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRY46328.1"
                     /translation="RMTFYGEFRGTPEAEKRVHESPATMTIPLIVLAAGSILVGFLGV
                     PEALGGANRFVSFLAPSLATAHPHHLSHATEYVLMALSVGVAAAGILLAWRWYGQKGA
                     TPEVPSAAAQAFYEKTGALGRLVENKWYVDEGIEAAVLSPFRKIGTFLWRGFDSLVID
                     GIVNASAFLVELTGDLVRFFTTGNVRNYALSFTLGVLVLALYVFLR"
BASE COUNT          321 a          526 c          644 g          197 t
ORIGIN      
        1 cggcgccgga gacggccgtg acgttccccg cgaagatccc gaggacgacg agcgccaggt
       61 ggctcaccga ggagtaggcc accagcttct tcatgtcctt ctgggccgcg gcgacccagg
      121 cgccgtagac gacgccgatc agggcgagga cggcgatcag aggtgtgtag cgcgccgccg
      181 ccccggggaa gagcgggatg gcgaaccgca ggaggccgta cgtaccgagc ttcaggagga
      241 cgccggccag gatgatcgag ccccccgtcg gcgcttcgac gtgggcgtcg gggagccacg
      301 tgtggagcgg ccagagcggg accttcacga ggaacgcgag ggcgaacgcc cagaagagga
      361 gcgcctgctc agaggcggtc aggctccagg cgccggacga agcggccggg atccactcga
      421 ggagcgagaa cgtcaggatg ccggtcgctt tcgcgtggac gcccgaggcg aagaggatcg
      481 ccacgaacat caggagggag ccggcgagcg tgaagaggac gaacttcatc gtcgcgtaga
      541 tgcgacgctt gccgccccag atcccgatga tgaagtacat cgggacgagc atcacttccc
      601 agaagacgta gaagaggacc gcgtcgagcg cgacgaggct cccgatcatc cccgtctcca
      661 ggacgaggaa ggcggcgacg tagccgcgca gcttcgggat cgtctgcccg aacgagaaga
      721 ccaggacgca gagcgtgagg agcgtcgtca ggacgacgag gatcagcgag aggccgtcga
      781 ccccgaggag gtagcggatc ccgaaccggg ggatccaggg gcggtccacg aggaggggcc
      841 ggaacgcctc gaggccggcg atggccgggt tgaaccgctg gaggacgagg accgacaggg
      901 cgaacgtcgc gccgctcgcg agcagggcga cggcccggat cgcgttcttc gccccgttcg
      961 ggaggaggag gaggagcagg gccccgagcg acggcaggaa gacgagcgtc gtcaggatgt
     1021 cggccccgag ccactggatg ggggtgctgg agtcgagcat gctctcctac cgcaggaaga
     1081 cgtagagcgc gaggacgagg acgccgagcg tgaagctcag cgcgtagttg cggacgttgc
     1141 cggtcgtgaa gaagcggacg aggtcgcccg tcagctcgac gaggaaggcc gacgcgttca
     1201 cgatcccgtc gatgacgagg gaatcgaacc cgcgccagag gaaggtcccg atcttccgga
     1261 agggggagag gaccgccgcc tcgatccctt cgtcgacgta ccacttgttc tcgacgaggc
     1321 ggccgagggc gcccgtcttc tcgtagaagg cctgcgcggc ggcgctcggc acctccggcg
     1381 tcgccccctt ctgcccgtac cagcgccagg ccaggaggat gccggccgcg gcgacgccga
     1441 cggagagggc catcaggacg tactccgtcg cgtgcgagag gtggtgcggg tgggccgtcg
     1501 cgagcgacgg cgcgaggaac gagacgaagc ggttcgcccc cccgagcgcc tcggggacgc
     1561 cgaggaagcc gacgaggatc gacccggccg cgaggacgat cagcggtatc gtcatcgtgg
     1621 cgggcgactc gtggacgcgc ttctccgcct ccggcgtccc gcggaactcc ccgtagaacg
     1681 tcatccgg
//
LOCUS       DAOTEZ010000660         1687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_66086_length_1687_cov_5.371324, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000660 DAOTEZ010000000
VERSION     DAOTEZ010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66086_length_1687_cov_5.371324"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="P5164_20525"
     CDS             complement(<1..379)
                     /locus_tag="P5164_20525"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019326511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HRY46329.1"
                     /translation="MSSDSNERMPAIFAAHGAPVLLDDAAWMGELAAWAKAMPRPASI
                     LMVSAHWEARPATLGATTPVSLVYDFYGFPEKFYRTVYPSPGAPALAARVRGLLNERG
                     IASTDEPARGLDHGAYVPLVAMYP"
     gene            complement(416..1330)
                     /locus_tag="P5164_20530"
     CDS             complement(416..1330)
                     /locus_tag="P5164_20530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRY46330.1"
                     /translation="MTRTANPTTPTIEAATKAAAPREEKVLSPRSVERLVAGTATSDG
                     AGVKLTRLLSQPLQRRLDPFLMLDAFGSDDPDDYIAGFPDHPHRGFETVTYMIAGRMK
                     HQDSAGHEGLLENGGVQWMTAGRGVVHSEIPQQEEGVMEGFQLWLNLPGRDKMQAPWY
                     RDFGAADLPRFALGGVTATVIGGESHGIAGAVTREATEPLVLDLRFEPGSRFEQPLAS
                     GHNAFVVVYRGSVTVGGKVLGPGRLAILENDPAADGVILEAAVASKALLVAGRPLGEP
                     IAQYGPFVMNTEEEIYQAVSDYRDGRLG"
     gene            complement(1356..>1687)
                     /locus_tag="P5164_20535"
     CDS             complement(1356..>1687)
                     /locus_tag="P5164_20535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRY46331.1"
                     /translation="GVPMYNLGNPVQLKAWIDAIARAGVTFRYTKSGPEGLLKGKTAY
                     VALARGGRYRGTPSDAQTPYLKTVLGFLGITDVRFIYAEGLNMGEEAARQGFAEAEAD
                     LETALAA"
BASE COUNT          216 a          599 c          608 g          264 t
ORIGIN      
        1 cggggtacat cgccacgagc ggcacgtaag ccccgtggtc gaggccgcgc gccggctcgt
       61 ccgtcgacgc gattccccgc tcattcagga ggccgcgcac ccgcgcggcg agcgcggggg
      121 cgccgggcga ggggtagacg gtgcggtaga acttctcggg gaagccgtag aagtcgtaga
      181 caagggagac cggcgtcgtc gcgccgagcg tcgccgggcg cgcctcccag tgggccgaga
      241 ccatcaggat gctcgccggc cgcggcatcg ccttcgccca cgcggcgagc tcccccatcc
      301 aggcggcgtc gtcgaggagg accggcgcgc cgtgcgcggc gaagatcgcg ggcattcgtt
      361 cgttcgagtc ggagctcatt ccgttcctct cctgtccgct ccggcgccgc gcggctcagc
      421 cgagccgtcc gtcccggtaa tcgctcaccg cctggtagat ctcctcttcc gtgttcatga
      481 cgaacgggcc gtactgggcg atcggctcgc cgagcggccg accggcgacg aggagcgctt
      541 tcgaagccac cgccgcctcg aggatgacgc cgtcggccgc ggggtcgttc tcgaggatcg
      601 cgagcctgcc gggcccgagc accttcccgc cgaccgtcac ggagcctcgg tagacgacga
      661 cgaaggcgtt gtgcccggac gcgagcggct gctcgaatcg ggagcccggc tcgaagcgga
      721 ggtcgaggac gagaggctcg gtcgcctcgc gcgtcaccgc gcccgcgatt ccgtggctct
      781 ctccgccgat gacggtggcc gtgacgccgc cgagcgcgaa gcgcggcagg tcggccgccc
      841 cgaagtcgcg gtaccagggg gcctgcatct tgtcgcggcc ggggaggttc agccagagct
      901 ggaacccctc catcacccct tcctcctgct gcgggatctc cgagtggacg acgccgcgcc
      961 cggccgtcat ccactggacg ccgccgttct cgaggagccc ttcgtggccc gcgctgtctt
     1021 ggtgcttcat ccgccccgcg atcatgtagg tcacggtctc gaagccgcgg tgcgggtggt
     1081 cggggaatcc cgcgatgtaa tcgtccgggt cgtcgctccc gaaggcgtcg agcatcagga
     1141 acgggtcgag ccgccgctgg agcggctgcg agagaaggcg cgtcagcttc acgcccgcgc
     1201 cgtcggaggt cgccgtgccg gcgacgaggc gctcgacgga gcgcggggag aggaccttct
     1261 cctctcgcgg ggcggcggct ttggtcgccg cctcgatcgt cggggtcgtc ggattcgcgg
     1321 tcctcgtcat gtacgtgtct ccctgcgcgt cgccgtcagg cggcgagcgc cgtctcgagg
     1381 tcggcttccg cctcggcaaa gccctgccgg gccgcctcct cgcccatgtt cagcccctcg
     1441 gcgtagatga agcggacgtc ggtgatgccg aggaagccga ggaccgtctt caggtagggc
     1501 gtctgcgcgt cgctcggcgt gccgcggtag cggccgcccc gcgcgagcgc gacgtacgcc
     1561 gtctttccct tcaggaggcc ttcggggccg ctcttcgtgt agcggaacgt gacgcccgcg
     1621 cgggcgatcg catcgatcca cgccttcagc tgcaccgggt tcccgaggtt gtacatcggg
     1681 accccga
//
LOCUS       DAOTEZ010000661         1680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_66537_length_1680_cov_5.690462, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000661 DAOTEZ010000000
VERSION     DAOTEZ010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66537_length_1680_cov_5.690462"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="P5164_20540"
     CDS             complement(<1..553)
                     /locus_tag="P5164_20540"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRY46332.1"
                     /translation="MQRISSHVLWLATAALDLGAITPFFYTFREREVILDLFEEYCGA
                     RLTLNCMRIGGLPVDLTPGWLEKVRAFTEPFEKNVDEYEELLTENRIWKQRTIGIGVL
                     DEKRCIEWGMTGPVLRAAGKAWDIRRAIPYEVYPELEFDIPVGKNADTYDRYLVRVAE
                     LRQSRRIVLQCLDWLEKNPGTEIR"
     gene            complement(906..>1680)
                     /locus_tag="P5164_20545"
     CDS             complement(906..>1680)
                     /locus_tag="P5164_20545"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRY46333.1"
                     /translation="GALRERASAMSEDKPIPPETPAAEPVSKASEAPAAPAEAPAAPE
                     PKPAPAVAAAPAAPAPAAPKPAAPAAPPKPAGPKATDASGKPLVAALAAAVPGSVLEA
                     YEFVGETTVKVEPGKLVEACRHLKDAEGFTYLVDLTAVDWKERDPRFDVLLLLHSFSR
                     GQERLRLKAAVGESCPSVTGVWLTANWMEREVFDMYGVRFDGHPDLRRILTWDGFQGY
                     PLRKDFPVEGIDTGAAIYPERYPEGGGPGPKDPNRKVLS"
BASE COUNT          224 a          572 c          621 g          263 t
ORIGIN      
        1 cgcggatctc cgtccccggg ttcttctcga gccagtcgag gcactggagg acgatccggc
       61 ggctctggcg gagctcggcg acgcgcacga ggtagcggtc gtaggtgtcc gcgttcttcc
      121 cgaccggaat gtcgaactcg agctcggggt agacctcgta ggggatcgcc cgacggatat
      181 cccaggcctt gccggcggcc cggaggacgg ggccggtcat cccccactcg atgcagcgct
      241 tctcgtcgag gacgccgatc ccgatcgtcc tctgcttcca gatccggttc tcggtcagga
      301 gctcttcgta ctcgtcgacg ttcttctcga acggctcggt gaacgcccgg accttctcga
      361 gccagccggg ggtcaggtcg accgggaggc cgccgatccg catgcagttc agcgtcaggc
      421 gggccccgca gtactcctcg aagaggtcga ggatcacctc ccgctcgcgg aacgtgtaga
      481 agaacggcgt gatcgccccg aggtcgagcg ccgccgtcgc gagccagagg acgtgcgacg
      541 agatccgctg catctcgtcg aggaggaccc ggatgtactg cgcccgcttc ggtacctcga
      601 caccgaggag cttctcgacg acgaggcagt acgcgtggtt gttcgtggag gccgcgacgg
      661 cgagcggatc gtctcggctc gccccgacgt ctcggtctcg aagagcttct ccgtgccgcg
      721 gtggaggtac ccgacgtcgg ggcgggccga gacgatccgc tcgccgtcga ccttcaggac
      781 gaggcgcagg acgccgtgcg tcgacgggtg ctggggcccg aagttgagct ccatctcgtg
      841 gagcccgagg aggggcggcc cctcgagggg ggggaactgc gcggcggtgc cgggagtcga
      901 ttcgctcatg agagcacctt ccggttcggg tccttgggtc ccggtccgcc cccctcgggg
      961 tatcgctccg ggtagatcgc cgcgccggtg tcgattccct cgacggggaa gtccttccgg
     1021 agcgggtaac cctggaatcc gtcccaggtc aggatccggc ggaggtcggg atgcccgtcg
     1081 aagcggaccc cgtacatgtc gaagacctcc cgctccatcc agttcgccgt cagccagacg
     1141 ccggtcaccg acgggcagct ctcgccgacc gcggccttca ggcggaggcg ctcctggccg
     1201 cgggagaagg aatggaggag gaggaggacg tcgaagcgcg ggtcgcgctc cttccagtcg
     1261 accgccgtca ggtcgacgag gtaggtgaac ccttccgcgt ccttcaggtg ccggcaggcc
     1321 tcgacgagct tgcccggctc gaccttcacg gtcgtctccc cgacgaactc gtacgcctcg
     1381 aggacggagc ccggcaccgc agcggcgagg gcggcgacga ggggcttgcc gctcgcgtcg
     1441 gtggctttcg gcccggcggg cttcggcggg gcggcggggg ccgccggctt cggcgcggcc
     1501 ggagcgggag ccgccggggc cgccgccacc gcaggggcgg gcttcggctc gggcgccgcc
     1561 ggggcctccg cgggcgccgc cggggcttcg gacgccttcg agaccggctc cgcggcggga
     1621 gtctcggggg ggatcggctt gtcttcgctc atcgcgctcg cccgttcacg aagcgcgccg
//
LOCUS       DAOTEZ010000662         1679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_66604_length_1679_cov_7.309729, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000662 DAOTEZ010000000
VERSION     DAOTEZ010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66604_length_1679_cov_7.309729"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="P5164_20550"
     CDS             complement(<1..853)
                     /locus_tag="P5164_20550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HRY46334.1"
                     /translation="MSTTVKNVLLWLVILVVILLLYKAVDYGRTAPPLSFTEFMEMVS
                     KGEIDRVVIRGGEVRGFKKGVPEGREPSYRTYAPPYDDLVNDLRKANVKITAEEPRDG
                     GLFMILLQWAPILFLIGLWVFIMRQMQTGGNRAMAFGKSKAKMLTPNQKKVTFKDVAG
                     CDESKDELSEIIDFLKDPAKFQKLGGKIPKGVLMMGPPGTGKTLLARAVAGEANVPFF
                     SISGSDFVEMFVGVGASRVRDLFEQGKKNAPCIIFIDEIDAVGRHRGAGLGGGHDERE
                     QTLNALLV"
     gene            complement(926..1489)
                     /locus_tag="P5164_20555"
     CDS             complement(926..1489)
                     /locus_tag="P5164_20555"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRY46335.1"
                     /translation="MNLLYDERFSPAEIAHRVQSVGQRISSDFPESPIVLIAILKGAA
                     VFASDLARSLPRSVRLEYIDVIRGSEEEIVDFHFVTPFNVRGADIIILKDVIRSGIIE
                     TYLADQLREEGPTSIRFAAIVDRPQERKSSLLVDYVLFPSEDGVLVGYGMEFAGEGGN
                     FPFIAKVRTPGDPPFALATGPIKVVRG"
     gene            complement(1486..>1679)
                     /locus_tag="P5164_20560"
     CDS             complement(1486..>1679)
                     /locus_tag="P5164_20560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46336.1"
                     /translation="EAAGRLLIRAARGTGRPPGRAQVGRVLDRVAAGETSFRETFAGR
                     RIAADRLTVRLTPPARLPA"
BASE COUNT          270 a          563 c          575 g          271 t
ORIGIN      
        1 cgacgaggag cgcgttcagg gtctgctcgc gctcgtcgtg cccgccgccg aggcccgcgc
       61 cgcggtgccg gccgacggcg tcgatctcgt cgatgaagat gatgcacggg gcgttcttct
      121 tcccctgctc gaagaggtcg cggacgcggc tcgcgccgac gccgacgaac atctcgacga
      181 agtcggagcc ggagatcgag aagaacggga cgttcgcctc gccggccacg gcgcgcgcca
      241 ggagcgtctt gccggtaccc ggggggccca tcatcaggac gcccttcggg atcttgccgc
      301 ccagcttctg gaatttcgcc gggtccttca ggaagtcgat gatctcggag agctcgtcct
      361 tcgactcgtc gcagccggcg acgtccttga acgtgacctt cttctggttc ggggtcagca
      421 tcttggcctt cgacttcccg aaggccatcg cgcggttccc gcccgtctgc atctgcctca
      481 tgatgaagac ccataggccg atcaggaaca ggatcggggc ccactggagg aggatcatga
      541 agagcccgcc gtcgcgcggc tcctccgccg tgatcttgac gttcgccttc cggaggtcgt
      601 tcacgaggtc gtcgtacggc ggggcgtacg tgcggtagga cggctcccgg ccttcgggaa
      661 cgcccttctt gaacccgcgg acctcgccgc cgcggatgac gacacggtcg atctccccct
      721 tcgagaccat ctccatgaac tccgtgaagc tcagaggggg agccgtccgg ccgtagtcca
      781 ccgccttgta gaggaggagg atgacgacga ggatcaccag ccagaggagg acgttcttga
      841 cggtcgtgct caaagggtca cccttcccgg caatctaagg cgccgggccg cccccgcgga
      901 gcgtcagcgc agcgggacat caagttcagc cccggacgac cttgatcggc ccggtggcga
      961 gcgcgaaggg agggtcgccc ggcgtcctca ccttcgcgat gaacgggaag ttgcctccct
     1021 cgccggcgaa ctccatgccg tagccgacga ggactccgtc ctccgacggg aacagcacgt
     1081 agtcgacgag gagcgagctc ttccgctcct gggggcggtc gacgatcgcg gcgaagcgga
     1141 tcgaggtcgg accctcctcg cggagctggt cggcgaggta ggtctcgatg attccgctcc
     1201 gaatcacgtc cttgaggatg atgatgtcgg ctccgcggac gttgaacggc gtcacgaagt
     1261 ggaaatcgac gatctcctcc tcgctccccc ggatcacgtc gatgtactcg agccgtacgg
     1321 agcggggcag ggagcgcgcc aggtcggacg cgaagacggc cgcgcccttc aggatcgcga
     1381 tgaggacgat cgggctttcc gggaagtcgc tcgagatcct ctggccgacc gactggacgc
     1441 ggtgagcgat ctcggcggga gagaaccgct cgtcgtacag gaggttcatg ccggcagtct
     1501 agcagggggc gtcaggcgga cggtcaaccg gtcggccgcg atccggcgcc cggcgaacgt
     1561 ctcccggaac gaggtctccc cggccgcgac ccggtccagg acccggccga cctgggcgcg
     1621 tccgggcggg cggcccgtcc cccttgccgc acggatcagg agacggcccg cggcctccc
//
LOCUS       DAOTEZ010000663         1665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_67599_length_1665_cov_6.442857, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000663 DAOTEZ010000000
VERSION     DAOTEZ010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67599_length_1665_cov_6.442857"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..932
                     /gene="nuoB"
                     /locus_tag="P5164_20565"
     CDS             219..932
                     /gene="nuoB"
                     /locus_tag="P5164_20565"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013505516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HRY46337.1"
                     /translation="MSDKKFPLPAARIEPPLAPGVPSTTGFDFLTTKLDWFVQWSHKN
                     SLWPMPFGTACCAIEFMSSVSSHYDLARFGAEVVRFSPRQADLMIVSGTITDKQAPIL
                     RKIYDQMPEPKWVISMGVCACTGGFYRAYHVVQGIDEIIPVDVYVAGCPPTPENLIYG
                     IIELQKRIQSGDLARHREAKTYVPIRVGTDPQVESLPAYSKAVMAAGRSSESGFAEMG
                     GSTEAEAAKAAEAAKGETR"
     gene            929..>1665
                     /locus_tag="P5164_20570"
     CDS             929..>1665
                     /locus_tag="P5164_20570"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRY46338.1"
                     /translation="MSAILAPATGVSFAPVLSRTFPDGSVRVEPRPASDVPTLVATPE
                     SWHAAALFLRDDPAASFDLLLDLVAVDRLKLDGPAAFRGEGRFQLNALLYSTKRNEHL
                     RLRVALPAAGPRVATLVDVWPAANWFEREAWDLMGVRFDGHPNLARLLTHNGFVGHAL
                     RKDYEAGQRWLCAEEDVLQTRLAANTDLPEDVFETTTLNFGPSHPATHGTLRIVVRLD
                     GERIVAAESECGYLHRCFEKMSETHTYT"
BASE COUNT          255 a          598 c          561 g          251 t
ORIGIN      
        1 gtctgcatct gcaacgggat caaggtgccg cgcgtcgagg aagcgatcga gaacggcgcg
       61 cggagcctgg aagaggtggc gcgggccacg ggctgcacga ccgggtcctg tcacggcgag
      121 cgctgcgtcc cggtcatcct cgagatgctc gacggaaagc gctgagagcc ctttacaatc
      181 gcgctcgtga tcggtttcac gagcaccggg ggtccccgat gagcgacaag aagttccccc
      241 tgccggccgc gcggatcgag ccgcccctcg cgcccggcgt cccctccacg acgggcttcg
      301 acttcctcac gacgaagctc gactggttcg tccagtggag ccataagaac tccctctggc
      361 cgatgccgtt cggcacggcc tgctgcgcca tcgagttcat gtcgtcggtg tcgtcgcact
      421 acgacctcgc ccggttcggg gccgaggtcg tccgtttctc gccgaggcag gccgacctga
      481 tgatcgtctc gggcacgatc accgacaagc aggccccgat cctccggaag atctacgacc
      541 agatgcccga gccgaagtgg gtcatctcga tgggcgtctg cgcctgcacc ggcggcttct
      601 accgcgccta ccacgtcgtc caggggatcg acgagatcat cccggtcgac gtctacgtcg
      661 cgggctgccc gccgacgccg gagaacctga tctacgggat catcgagctc cagaagcgga
      721 tccagtcggg cgacctcgcc cggcaccgcg aggcgaagac gtacgtcccg atccgcgtcg
      781 gcacagaccc gcaggtggag tcgctgccgg cctactcgaa ggcggtcatg gcggccggac
      841 ggagctcgga gtccggcttc gcagagatgg gcggctcgac ggaagcggag gcggcgaagg
      901 ccgcggaagc cgcgaaggga gagacgcgat gagcgcgatc ctcgccccgg cgacgggcgt
      961 ctcgttcgca ccggtcctgt cgcggacgtt tccggacggc tccgtccgcg tcgagccgcg
     1021 ccccgcctcc gacgtcccca ccctggtcgc gacgccggag tcctggcacg ccgccgcgct
     1081 cttcctccgg gacgacccgg ccgcgtcctt cgacctcctc ctcgatctcg tcgccgtcga
     1141 ccgcttgaag ctcgacggcc cggcggcctt ccgcggcgag gggcgtttcc agctgaacgc
     1201 gctcctctat tcgacgaagc ggaacgagca cctccggctc cgggtcgccc tcccggccgc
     1261 cggcccgcgc gtcgcgacgc tcgtggacgt ctggccggcg gcgaactggt tcgagcgcga
     1321 ggcgtgggac ctgatgggag tccgcttcga cggccatccg aaccttgcgc ggctcctcac
     1381 gcacaacggc ttcgtcgggc acgccctccg gaaggactac gaggcgggcc agcgctggct
     1441 ctgcgccgag gaggacgtcc tccagacccg tctcgccgcg aataccgacc tccccgagga
     1501 cgtcttcgag acgacgacgc tgaacttcgg cccgtcgcat cccgcgacgc acgggacgct
     1561 ccggatcgtc gtgcggctcg acggcgagag gatcgtggcg gccgagtcgg agtgcgggta
     1621 cctgcaccgc tgcttcgaga agatgtcgga gacgcacacg tacac
//
LOCUS       DAOTEZ010000664         1664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_67651_length_1664_cov_5.683033, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000664 DAOTEZ010000000
VERSION     DAOTEZ010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67651_length_1664_cov_5.683033"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="P5164_20575"
     CDS             complement(<1..1087)
                     /locus_tag="P5164_20575"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HRY46339.1"
                     /translation="MPGFLHVTAADVSAVGPEAFLCVVGIALVLLDAFAKSLRPSFPY
                     LTLGGIAVANFLGGYPSGSAFAGAIETSELTRFVDLLALAAVALATLGGGALLARDRR
                     NQGESYALLLWSAAGLMLMVKGDDLLVVFIGLELMSIALYVLAAWYREVAEATEAAMK
                     YFLMGALASTFLLLGVALVYGRLGSTRLTALKGALASGEGFSDPMLQVGLLAILAALA
                     FKIALVPFHSWAPDVYQGMTTPAVAFLSTAPKAAAVVVAVKVLGTLAPSGLGEPWRPL
                     LSLLAVLSILFGNVVALAQRDLKRMLAYSGIAQMGYVAIGLATFDGAAFEGILVFLAG
                     YLATNIAAFLAVAALSNGEKEAHPLADL"
     gene            complement(1116..>1664)
                     /locus_tag="P5164_20580"
     CDS             complement(1116..>1664)
                     /locus_tag="P5164_20580"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRY46340.1"
                     /translation="ALVVLGIFAGNVTAVSGAVMQMVGHGLTTGLLFLLVGVLYERRH
                     TREMADYGGVASQVPVTTTLFVIAMLGSVGLPGLNGFVGEFLILAGVFKANVWWAAFA
                     ATGLILGAIYLLTLTQKVFWGPNRVEANLGLTDINGWELAGALPMIVLVVVLGVWPQP
                     VLDLVRNSVETVVQIASVRMVP"
BASE COUNT          300 a          530 c          648 g          186 t
ORIGIN      
        1 cgaggtcggc gagcgggtgg gcctctttct cgccgttcga gagagcggcc accgcgagga
       61 acgccgcgat gttcgtcgcg aggtagccgg cgaggaagac gaggatcccc tcgaacgcgg
      121 cgccgtcgaa cgtggcgagg ccgatcgcga cgtagcccat ctgggcgatg cccgagtagg
      181 cgagcatccg cttcaggtcg cgctgcgcga gcgccacgac gtttccgaag aggatcgaga
      241 ggaccgccag gagggagagg agcggccgcc agggctcacc gaggcccgac ggcgcgagcg
      301 tcccgagcac cttgacggcg acgacgacgg ccgccgcctt cggggcggtg gagaggaacg
      361 cgacggccgg ggtcgtcatc ccctggtaga cgtcgggcgc ccaggagtgg aaggggacga
      421 gggcgatctt gaacgcgagg gccgcgagga tcgcgaggag cccgacctgg agcatcgggt
      481 cggagaagcc ctcgcccgag gcgagcgccc ccttcagcgc cgtgaggcgg gtcgagccga
      541 gacgtccgta gacgagcgcg acgccgagca ggaggaatgt ggaggcgagc gcccccatca
      601 ggaagtactt catcgccgcc tcggtcgcct cggcgacctc ccggtaccac gcggcgagga
      661 cgtagagcgc gatcgacatc agctcgaggc cgatgaagac gacgaggagg tcgtcgccct
      721 tgaccatgag catcagcccc gcggcggacc agaggaggag cgcgtaggac tccccctggt
      781 tcctccggtc gcgggcgagg agcgccccgc cgccgagcgt cgcgagggcg acggcggcga
      841 gcgcgaggag gtcgacgaag cgggtcagct cggaggtctc gatcgccccg gcgaaggcgc
      901 tcccggacgg gtagccgccc aggaagttcg cgacggcgat gccgccgagc gtgaggtagg
      961 ggaaggaggg gcggaggctc ttcgcgaacg cgtcgaggag gacgagagcg atgccgacga
     1021 cgcagaggaa ggcctcgggg ccgacggcgg agacgtcggc ggcggtgacg tgaaggaagc
     1081 cgggcacgtt cagcagctcc tctcggcggc ggacgtcacg ggaccatccg gacggacgcg
     1141 atctgcacga ccgtctcgac ggagttgcgg acgaggtcga ggaccggctg cggccagacc
     1201 ccgaggacga cgacgaggac gatcatcggg agcgcgcccg cgagctccca tccgttgatg
     1261 tcggtcaggc cgaggttcgc ctcgacccgg ttcggtcccc agaagacctt ctgcgtgagc
     1321 gtcaggaggt agatcgcgcc gaggatgagg ccggtcgccg cgaacgcggc ccaccagacg
     1381 ttcgccttga agaccccggc gaggatcagg aactccccga cgaagccgtt caggccggga
     1441 aggccgacgg agccgagcat cgcgatcacg aagagcgtcg tcgtgacggg gacctgcgag
     1501 gcgacgccgc cgtagtcggc catctctcgc gtgtggcgcc gctcgtagag gacgccgacg
     1561 aggaggaaga ggaggccggt cgtcaggccg tgccccacca tctgcatcac ggcgccggag
     1621 acggccgtga cgttccccgc gaagatcccg aggacgacga gcgc
//
LOCUS       DAOTEZ010000665         1662 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_67763_length_1662_cov_4.602987, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000665 DAOTEZ010000000
VERSION     DAOTEZ010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67763_length_1662_cov_4.602987"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="P5164_20585"
     CDS             complement(<1..1189)
                     /locus_tag="P5164_20585"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRY46341.1"
                     /translation="MKLPGFAYDARRRVATFYCFVPGTDGRKRHKRKLTDVPSRAEAI
                     RLWTDFREELEREALEPAKFTRPPTLAEFVSERFVEMTARLRPATRRYYGDFLRRHLI
                     PRLGDVPMDQLTSQRVNALIGSMISEGWCPKRGRERKPHGCTPACVRRPYAGSTINGA
                     AHVLRVIIGWAVELDVLDGSPVKKRVKWARESRPELELSLEERHRFLSAFEDEALFQA
                     YLGRIRTPPKIVPHPSGGMKRVGGGRLPDGKAAKEIFRRLQHYRPFFRILLDTGLRLS
                     DALDLRWVNVSLDENVIRLVTRKKEKAVKLPLSDAVRTVLETLKKRPVISEFVFLDED
                     NKPLTKEKVARKFAMAKAVAGITRRLRIHDLRHTFASTLVSSGVNLKVVSDLLGHTDI
                     ATTA"
     gene            complement(1209..1439)
                     /locus_tag="P5164_20590"
     CDS             complement(1209..1439)
                     /locus_tag="P5164_20590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46342.1"
                     /translation="MAIKASEVARRIGVKTGTLGKWRRQGKGPKGWYALSATVVVYPE
                     TEVQSFLREQRAALDRQDRSTAPPVTPEGPKQ"
BASE COUNT          279 a          551 c          551 g          281 t
ORIGIN      
        1 gggccgtcgt cgcgatatcg gtatgtccga gaaggtcgga gacgactttg aggttcacac
       61 cggacgagac gagggtcgag gcgaacgtgt ggcggaggtc gtgaatccgg agcctccggg
      121 tgatcccggc aacggccttc gccatcgcga acttccgagc gaccttctcc ttcgtgagcg
      181 gcttgttgtc ctcgtcgagg aacacgaact ccgagatgac cggccgcttc ttcagggtct
      241 cgaggacggt ccggacggca tcggagagcg gtagcttgac cgccttctcc ttctttcggg
      301 tcacgagccg gatgacgttc tcgtcgagcg acacgttcac ccagcggaga tcaagcgcgt
      361 ccgagaggcg aagccccgtg tccaggagga tccggaaaaa cggacggtag tgctgaaggc
      421 gacggaagat ctccttcgcg gccttgccgt ccgggagcct cccgccgccg acgcgcttca
      481 tgccgcccga cgggtgcggc acgatcttcg gcggggtgcg gatgcggccg aggtaggcct
      541 ggaagagggc ctcgtcctcg aaggccgaga ggaagcgatg gcgctcctcc aaggagagct
      601 cgagctccgg tcgggattcg cgggcccact tcaccctctt ctttacgggg ctcccgtcga
      661 ggacatcgag ctcgaccgcc cagccgatga tgacccgaag aacatgagca gcgccgttga
      721 tcgtcgaccc ggcgtacggc cgcctcacgc aggccggcgt gcagccgtga ggctttcgct
      781 cccggccgcg cttcgggcac cacccctccg agatcatcga accgatgagt gcattcacgc
      841 gctgggacgt cagctggtcc atcggcacgt cgccgagcct cgggatgagg tggcgacgga
      901 ggaagtcgcc gtagtagcgc cgggtcgcgg gtcggagacg ggcggtcatc tcgacgaagc
      961 gctccgagac gaactctgcg agcgtggggg gccgggtgaa cttcgccggc tcgagcgcct
     1021 cgcgttcgag ctcctcgcgg aaatcggtcc agagccggat cgcctcggcc cgggacggga
     1081 cgtcggtgag cttccgcttg tggcgcttcc ggccgtccgt tcccgggacg aagcagtaga
     1141 acgtcgcgac ccggcgacgc gcgtcgtagg cgaagccggg gagcttcatc ggtcaccccc
     1201 gccgagcgct actgcttcgg cccctccggc gtcaccggcg gcgcagtcga ccggtcttgc
     1261 cggtcgagag ccgcccgctg ctcgcggagg aagctctgca cctcggtctc cgggtagacg
     1321 acgacggtcg ccgagagggc gtaccacccc ttcggcccct tgccctgtcg acgccacttc
     1381 ccgagcgtcc ccgtcttgac cccgattcgc cgggcaactt cggatgcctt gatcgccatg
     1441 cgacacctcc gcgcgaagca tccgcggaca tcgcaggacc gaccgggacg gcgtcgaaat
     1501 caccgtcccc gaaaagagaa ttagatactt tgttagatac ccccccaaaa agccgcagga
     1561 cgcctcttcg gcgtctcccg ctaaaccttt tgttgtgagt attttagatg gtcgggacgg
     1621 cgggattcga acccgcgacc ccttgaaccc cattcaagta cg
//
LOCUS       DAOTEZ010000666         1650 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_68640_length_1650_cov_4.787461, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000666 DAOTEZ010000000
VERSION     DAOTEZ010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68640_length_1650_cov_4.787461"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="P5164_20595"
     CDS             complement(<1..421)
                     /locus_tag="P5164_20595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY46343.1"
                     /translation="MSAAPPLPAPEAPPEIRLSGLRKAFGPKVVLDGLDLTIAKGDSI
                     VILGKSGTGKSVLLKHMIGLLAPDSGTVEVEGEDFWAASLSRRDEMRRRFGMSFQEGA
                     LFDSMSVGENIAFALKRHTRMTPAEVRDFMANHAPKAW"
     gene            complement(418..1185)
                     /locus_tag="P5164_20600"
     CDS             complement(418..1185)
                     /locus_tag="P5164_20600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY46344.1"
                     /translation="MALLEAFGRLALVALEFHGRCVALLGLTIRGLFRRPFDGRAIAT
                     QVVRVGVEGLPVVLLTAVFTGAVLALQTYTGFQRFHAEAWVGSVVSLSMLRELAPVLT
                     GLMVAGRSGSAMAAEIGSMRVTEQIDALVALATDPVQYLFVPRILAGIIVLPMLVVLA
                     NAFGVMGGYLVAVRLLGANPVVYMENSFQFLDLNDLWSGLIKAAAFGLLITLIGCQQG
                     FDTRGGAEGVGRSTTAAVVLGSLAILVSDFFLTKVLF"
     gene            complement(1271..>1650)
                     /locus_tag="P5164_20605"
     CDS             complement(1271..>1650)
                     /locus_tag="P5164_20605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HRY46345.1"
                     /translation="GSRQKYENRTQEVGAYSRGLKALAKELDVPVIALSQLSRRTEQR
                     GAEKEPQLSDLRESGAIEQDADLVIFLSRPEYYDKDTPDKNVCEVIVAKQRNGPTGRF
                     SLGWSGETTRFSDLAQVPEGSGP"
BASE COUNT          265 a          583 c          579 g          223 t
ORIGIN      
        1 cccacgcttt cggcgcgtgg ttggccatga agtcgcgcac ctcggccggc gtcatacggg
       61 tgtggcgctt cagcgcgaag gcgatgttct ccccgacgct catcgagtcg aacagggccc
      121 cctcctggaa cgacatcccg aaacggcgcc tcatctcgtc ccggcgcgag agcgaggcgg
      181 cccagaagtc ctcgccttcg acctcgaccg tccccgagtc cggcgccagg agcccgatca
      241 tgtgcttcag gaggacgctc ttcccggtcc ccgacttgcc gaggatgacg atcgagtcgc
      301 ccttcgcgat cgtgaggtcg agcccgtcga ggacgacctt cggcccgaag gccttccgca
      361 gcccggagag gcggatctcg ggcggcgcct ccggcgcggg aaggggcggc gccgcgctca
      421 gaacagcacc ttcgtcagga agaagtcgga gaccaggatc gcgagcgagc cgaggacgac
      481 ggccgccgtc gtggagcgtc ccacgccctc cgccccgccc cgggtgtcga acccctgctg
      541 gcagccgatg agcgtgatga ggagcccgaa cgcggccgcc ttgatgagac ccgaccagag
      601 gtcgttcagg tcgaggaact ggaacgagtt ctccatgtag acgaccgggt tcgctcccag
      661 cagcctcacg gcgacgaggt agcctcccat caccccgaac gcgttcgcca ggacgacgag
      721 catcggcagg acgatgatcc cggcgaggat ccgcgggacg aacaggtact ggacggggtc
      781 cgtcgcgagg gccacgagcg cgtcgatctg ctcggtcacg cgcatcgagc cgatctcggc
      841 ggccatcgcg ctcccggagc gcccggccac catgagaccc gtgaggaccg gcgcgagctc
      901 ccggagcatc gagagcgaga cgaccgagcc gacccacgcc tcggcgtgga acctctggaa
      961 gccggtgtag gtctggaggg cgaggaccgc gcccgtgaag accgccgtca ggaggaccac
     1021 ggggagccct tcgacgccca cgcggacgac ctgcgtcgcg atggcccggc cgtcgaaggg
     1081 gcggcggaag agcccgcgga tcgtcagccc gagcagcgcg acgcagcggc cgtggaactc
     1141 gagcgcgacg agcgccagcc ttccgaaggc ctcaaggagg gccatcgccc ccccgtccgg
     1201 aagcggactc gcgccgcggc ggattcacgg gatcacctct cctccgatga tacgcgggcg
     1261 tggcccgcgc ctacggtccg gagccctcgg ggacctgggc gaggtcggag aagcgcgtcg
     1321 tctcgccgga ccagccgagc gagaagcggc ccgtcgggcc gttccgctgc ttcgccacga
     1381 tcacctcgca gacgttcttg tccggcgtgt ccttgtcgta gtactcgggg cgcgagagga
     1441 agatgacgag gtcggcgtcc tgctcgatgg cgcccgactc gcggaggtcc gagagctggg
     1501 gctccttctc cgcgccgcgc tgctccgtcc ggcgcgagag ctgcgagagc gcgatgacgg
     1561 ggacgtccag ctccttcgcg agggccttca ggccccgcga gtaggcgccg acctcctgcg
     1621 tccggttctc gtacttctgg cgcgagccca
//
LOCUS       DAOTEZ010000667         1649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_68709_length_1649_cov_4.499373, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000667 DAOTEZ010000000
VERSION     DAOTEZ010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68709_length_1649_cov_4.499373"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..834
                     /locus_tag="P5164_20610"
     CDS             28..834
                     /locus_tag="P5164_20610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRY46346.1"
                     /translation="MSEALRLERPRAGVVRVVLSRPEVRNAFDDGLIAEVTAAFEQLA
                     SDASTRVVVLAGEGKAFCAGADLTWMKRMVTWGEEENRRDAEALGRMFLAIDSCPKPV
                     VGRIHGAALGGGAGLVAVCDVAVCADGTLLGTTEVRLGIVPAVISPFVVRKVGESHAR
                     RWFLTGERLDAEAALRAGLVHVVVPEAELDAEVDRVVDALLLGGPEALAVSKGLAKRM
                     GRVPTDEALAEAACIIATRRVSSEGQEGMEAFLAKRPPAWAPEPGRRSGS"
     gene            831..>1649
                     /locus_tag="P5164_20615"
     CDS             831..>1649
                     /locus_tag="P5164_20615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019435659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HRY46347.1"
                     /translation="MNLRPFRKVLVANRGEIAVRVLTACRTLGYGTVAVASEADVASR
                     HARLADELVLLGPAPARESYLAASRILDAARATGAEAIHPGFGFLSENAAFARAVIDA
                     GLVWIGPPPDAIEAMGLKVPSRERMRTAGVPVVPGTHDLTPEGIAKVGFPLVVKASAG
                     GGGKGMRVVRAEAELAPAVESCRREAEAAFGDPTLYAERYLEHPRHIEIQVFGDVHGR
                     VVALGERECSLQRRHQKVVEESPSPVVTPDLRARMSEAAVAAAKAPRHRLVPRVE"
BASE COUNT          200 a          587 c          630 g          232 t
ORIGIN      
        1 ggccaccggg tcgatcgagg tgctgcgttg agcgaggcgc tccgcctgga gcgcccgcgc
       61 gccggcgtcg tgcgcgtcgt cctctcgagg cccgaggttc ggaacgcgtt cgacgacgga
      121 ctgatcgcgg aggtcaccgc ggctttcgag cagctcgcgt cggatgcctc cacgcgcgtc
      181 gtcgtcctcg caggcgaggg gaaggccttc tgcgccggcg ccgacctgac ctggatgaag
      241 aggatggtga cgtggggcga ggaggagaac cggcgcgacg ccgaggcgct cggccggatg
      301 ttcctcgcga tcgactcctg cccgaagccg gtcgtcggcc gtatccacgg cgcggccctc
      361 ggcggcgggg cggggctcgt ggccgtctgc gacgtggccg tgtgcgccga cggcaccctc
      421 ctcgggacga ccgaggtccg gctcggcatc gtcccggccg tcatctcgcc gttcgtggtc
      481 cggaaggtcg gcgagtcgca cgcgcgccgc tggttcctga cgggcgagcg gctcgacgcc
      541 gaggcggcgc ttcgtgccgg gctcgtccac gtcgtcgtcc cggaggccga gctcgacgcc
      601 gaggtcgatc gcgtcgtcga cgccctcctc ctcggcgggc cggaggcgct cgccgtctcg
      661 aagggcctcg cgaagaggat ggggcgcgtc ccgacggacg aagccctcgc ggaggcggcc
      721 tgcatcatcg cgacgcgccg cgtctcttcg gaagggcagg agggaatgga ggcgttcctc
      781 gcgaagcggc ccccggcgtg ggcgccggag ccgggacgga ggagcggctc gtgaaccttc
      841 gtcctttccg gaaggtcctc gtcgcgaacc ggggagagat cgccgtccgc gtcctgacgg
      901 cctgccgcac gctcgggtac ggcacggtcg ccgtcgcgtc cgaggccgac gtcgcgtcgc
      961 ggcacgcccg tctggccgac gagctcgtcc tgctgggccc ggcgccggcg cgcgagtcgt
     1021 acctcgccgc gtcgaggatt ctcgacgccg cgcgcgccac gggcgccgag gcgatccatc
     1081 ccggcttcgg cttcctctcc gagaacgccg cgttcgcgcg cgcggtgatc gacgccgggc
     1141 tcgtctggat cggtccgcct cccgacgcga tcgaggcgat ggggctcaag gtcccgtcgc
     1201 gcgagcgcat gaggacggcg ggagtcccgg tcgtccccgg cacgcacgac ctgacccccg
     1261 aggggatcgc gaaggtcggc tttccgctcg tggtgaaggc ctcggccggc ggcggcggaa
     1321 agggaatgcg cgtcgttcgc gccgaggccg agctcgcccc ggccgtcgag tcgtgccgcc
     1381 gcgaggcgga ggccgccttc ggcgacccga cgctctacgc cgagcgctac ctcgagcacc
     1441 cccgtcacat cgagatccag gtcttcggtg acgtccacgg gcgcgtcgtg gcgctgggcg
     1501 agcgggaatg ctcgctccag cggcggcacc agaaggtcgt cgaggagagc ccctcgccgg
     1561 tcgtcacgcc ggatctccgc gcgcggatga gcgaagccgc cgtggcggcg gcgaaagccc
     1621 cgcgacatcg actggttccg cgggttgaa
//
LOCUS       DAOTEZ010000668         1646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_68930_length_1646_cov_5.207417, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000668 DAOTEZ010000000
VERSION     DAOTEZ010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68930_length_1646_cov_5.207417"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="P5164_20620"
     CDS             complement(<1..897)
                     /locus_tag="P5164_20620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type B"
                     /protein_id="HRY46348.1"
                     /translation="MSGSKKARTVLDALVQAVRSVAPESADAGSPPAAILWTDKENQW
                     AALLPALKKALPELLVLGAWAPEERTGPAIWLRCVLGGTIPEAALPKGAVPILYLPGV
                     SRGDLRAVEECPRLLQPLAELQYRGVYFSQKSGKDWTIRAFLGGKDGLDLDVASDEAT
                     SRAMLAAVRELGETPVRQLSGKRLEAMDFNELVAPDMVRSLLSWMSDPKGTRSAWTAE
                     EWSAFVHECKALLRFDPERDGELEAAEALGRREHGWEKVWQRYEEAPDKYPGVAPILE
                     KAEPKKDAGSLYYRAESWPGATD"
     gene            complement(894..>1646)
                     /locus_tag="P5164_20625"
     CDS             complement(894..>1646)
                     /locus_tag="P5164_20625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008869234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HRY46349.1"
                     /translation="LGYRWPDQEKDDLAALCDRDGIVCIPPVGGEQPAADRLRALLAA
                     AYGKKWKGTTEAELLASVGFAGKSLDEWLRDGFFEQHSKLFHHRPFVWHVWDGRKKDG
                     FHALVNYHKLDRKLLEKLTFTTLGGWIQQLRQAEKRGEGTAEARLAAALDLQERLEAI
                     LEGEKPYDIFVRWKPIEEQPIGWEPDLNDGVRLNIRPFVEADVLRKKPNIKWEKDRGK
                     NPPGAPWGEERLNDLHLSLDEKRAARKKKGRA"
BASE COUNT          215 a          614 c          539 g          278 t
ORIGIN      
        1 atccgtcgct ccgggccacg actcggcccg gtagtagagc gacccggcgt ccttcttcgg
       61 ctcggccttc tcgagaatag gggcgacccc cggatacttg tcgggcgctt cttcgtaccg
      121 ctgccagacc ttctcccacc cgtgctcccg cctgccgagc gcctcggcgg cctcgagttc
      181 gccgtcgcgc tcggggtcga agcggaggag cgccttgcac tcgtgaacga acgcactcca
      241 ctcctcggcg gtccaagccg atctcgtgcc ctttgggtcg ctcatccacg agaggaggct
      301 ccggaccatg tcgggagcca ccagctcgtt gaagtccatc gcctctagcc gcttccccga
      361 gagctggcgc acgggcgtct cgcccagctc cctaaccgcc gcgagcatcg cgcggctcgt
      421 cgcctcgtcc gaggcgacgt cgaggtcgag gccgtccttc ccgccgagga acgcgcgaat
      481 cgtccagtcc ttcccgctct tctgcgagaa gtagacgccc cggtactgaa gctccgcgag
      541 cggctgcaag agacgcgggc actcctccac ggcgcgaagg tccccgcggg agacgccggg
      601 gaggtagagg atcggcacgg cccccttcgg cagcgccgcc tccgggatcg tcccgccgag
      661 gacgcaccgg agccagatgg ccggccccgt ccgctcctcc ggcgcccagg cgccgaggac
      721 gagaagctcc gggagggcct tcttcaaggc cggcaggagc gccgcccact ggttctcctt
      781 gtcggtccag aggatcgcgg ccggcggcga gccggcgtcc gcgctctccg gcgcgacgct
      841 tcggacggcc tgcacgagag cgtcgaggac cgtccgcgcc ttcttcgatc cgctcacgcc
      901 cgccccttct tcttccgcgc cgcccgcttc tcgtcgagcg agaggtggag gtcgttgagg
      961 cgctcctcgc cccacggcgc gccgggcggg ttcttgccgc ggtccttctc ccacttgatg
     1021 ttcggcttct tgcggaggac gtcggcctcg acgaaggggc ggatgttgag ccgcacgccg
     1081 tcgttcaggt ccggctccca gccgatcggc tgctcctcga tcggcttcca gcggacgaag
     1141 atgtcgtagg gcttctcgcc ctcgaggatc gcctcgagcc gctcctggag gtcgagcgcc
     1201 gccgcgaggc gcgcctcggc cgtcccctcg cctcgcttct ccgcctgccg gagctgctgg
     1261 atccagccgc cgagcgtcgt gaacgtcagc ttctcgagga gctttcggtc gagcttgtgg
     1321 tagttgacga gggcgtggaa accgtccttc ttccgaccgt cccacacgtg ccagacaaac
     1381 ggccggtgat ggaagagctt cgagtgctgc tcgaagaacc cgtcccgcag ccactcgtcg
     1441 aggctcttcc ccgcgaagcc gaccgaggcg agaagctctg cctccgtcgt ccccttccac
     1501 ttcttgccgt aggccgccgc gaggagcgcc cggagcctgt ccgccgccgg ctgttcgccg
     1561 ccgaccggcg ggatgcagac gatgccgtcg cggtcacaga gggcggcgag gtcgtccttc
     1621 tcctggtccg gccagcggta gccgag
//
LOCUS       DAOTEZ010000669         1638 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_69495_length_1638_cov_5.078332, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000669 DAOTEZ010000000
VERSION     DAOTEZ010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69495_length_1638_cov_5.078332"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1546
                     /locus_tag="P5164_20630"
     CDS             <1..1546
                     /locus_tag="P5164_20630"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRY46350.1"
                     /translation="VAATFRGRTLVLRRRNEELERVVAERTADLATANGHLRNAQGEI
                     ARLLEASPAPSENVASWSQALGADVASAIGAERIGIWEVRDDAVVPLSDAGLAPPRID
                     DLRGGAPGAPRPLPSEEGSVVVPVSGMSSELRGALLVQGPAVVWGETERRLVGGFAHQ
                     LGAALDMAHMRRQLSSLREKREATLREMHEQGIATLQVCPLCGRCYDQAAEECAVDGS
                     PLDTPRPLPYELLGRYRFERVLGQGGMATVFSARDTRLSRDVAIKLIRPEHFGDSELK
                     ARFEHEARTIARISHPGVIGLFDSGELPDGTAFLVMEMLRGHDLAHVLLECGTGTPRQ
                     VAQLAREAGAALAAAHAAGVVHRDVKPGNLFLADDPAGFRVKVLDFGLAKELAHEKGL
                     TRTGMVVGTPEYMSPEQVHGWNVDARTDVYSLGAVCYEALLGRRAIRGEDLGRIMANV
                     IDEVPPPASSVLPGVPEAASVALAVALAKDVFERPSDVAAWSETLAASLETMPDGLAS
                     GWTRID"
BASE COUNT          206 a          635 c          586 g          211 t
ORIGIN      
        1 ggtggccgcc acgttccgcg ggaggacgct cgtccttcgc cggaggaacg aggagctcga
       61 gcgcgtcgtc gcggagcgga cggccgacct cgccaccgcg aacggccacc tccggaacgc
      121 ccagggcgag atcgcgcggc tcctcgaggc gagcccggcg ccgtccgaga acgtggcgag
      181 ctggtcccag gcgctcggcg ccgacgtcgc gagcgcgatc ggcgcggagc ggatcggcat
      241 ctgggaagtg cgggacgacg ccgtcgtccc cctctccgac gccggcctcg cccctccgcg
      301 aatcgacgac ctccgcggcg gggcgccggg cgccccgcgc ccgctcccgt cggaggaagg
      361 gtcggtcgtc gttcccgtct ccgggatgag ctccgagctc cgcggcgcgc tcctcgtcca
      421 gggcccggcg gtcgtctggg gcgagaccga acggcggctc gtgggcgggt tcgcgcacca
      481 gctcggcgcc gcgctcgaca tggcccacat gcgccggcag ctctcctcgc tccgcgagaa
      541 gcgggaggcg acgctgcggg agatgcacga gcaggggatc gcgacgctcc aggtctgccc
      601 tctctgcgga cgctgctacg accaggcggc cgaggagtgc gccgtcgacg gctccccgct
      661 cgacacgccc cggccgctcc cctacgagct cctcggccgc taccggttcg agcgcgtcct
      721 cgggcagggc gggatggcga cggtcttctc ggcccgcgac acgcgcctct cgcgcgacgt
      781 cgcgatcaag ctgatccggc cggagcactt cggcgactcc gagctcaagg cccgtttcga
      841 gcacgaggcg cggacgatcg cccggatctc gcaccccggc gtcatcggcc tcttcgactc
      901 cggcgagctt ccggacggca ccgcgttcct cgtgatggag atgctccgcg ggcacgacct
      961 ggcgcacgtc ctcctcgagt gcggcaccgg caccccgcgc caggtcgccc agctcgcgcg
     1021 cgaggccggc gcggcgctcg ccgcggccca cgcggcaggc gtcgtccacc gcgacgtcaa
     1081 gcccgggaac ctcttccttg ccgacgaccc ggcgggcttc cgcgtgaagg tgctcgactt
     1141 cggcctcgcc aaggagctgg cgcacgagaa ggggctgacg cgcaccggga tggtcgtcgg
     1201 aacgccggag tacatgtccc ccgagcaggt gcacggctgg aacgtcgacg cgcgcaccga
     1261 cgtctactcc ctcggcgccg tctgctacga ggcgctcctc ggccgccggg cgatccgggg
     1321 cgaggacctc gggcggatca tggcgaacgt catcgacgag gtcccgcccc ccgcctcgtc
     1381 ggtcctcccc ggagtcccgg aggcggcctc cgtcgccctc gcggtggccc tcgcgaagga
     1441 cgtcttcgag aggccgtccg acgtcgccgc ctggtcggag accctcgccg cctcgctcga
     1501 gacgatgccg gacggcctcg cctcgggctg gacgagaatc gactgaagcg cgcggcgcgg
     1561 ccgcgcccag caggatcggc atgagacgaa gccgcctgct cccggtcgtc gcgctcgccc
     1621 tcgcggcgat cgtccccg
//
LOCUS       DAOTEZ010000670         1631 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_70001_length_1631_cov_8.483503, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000670 DAOTEZ010000000
VERSION     DAOTEZ010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70001_length_1631_cov_8.483503"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="P5164_20635"
     CDS             <1..297
                     /locus_tag="P5164_20635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46351.1"
                     /translation="ANRRGITKYHRESDAVFAGNACAIWRYADDPEVLNSPTTTYWMA
                     PALGGVVIRKVRTVPKDDGTEEVTYFELTHLRAGVDPEGFRVPEGFRKEGTGGR"
     gene            389..1240
                     /locus_tag="P5164_20640"
     CDS             389..1240
                     /locus_tag="P5164_20640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46352.1"
                     /translation="MKPYAAILASALIAALPLAAQTPVDPKSPIPGFPDGRGPKGPVS
                     IKLISPAADEVIPMPAAEPGKPEPKGARVEVKVAVENYEIFKDEATGKGQHVQLVLDG
                     RALPNWYAIDKAWIFPALPKGTHTLLAFLQRPWNETIREAGAFAAVTFHVGEKNGKPA
                     FDPAQPSITVAAPRGKFEGEQATKILFDVFVTGCTVGPATDPEACQIIYRLNQDPQQV
                     LTEWKPVFWENVPPGKHSFIAALYKGEKRIEAPFALTQGFFEVVAAPAPAAAPAAAPA
                     APATPAG"
     gene            1285..>1631
                     /locus_tag="P5164_20645"
     CDS             1285..>1631
                     /locus_tag="P5164_20645"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HRY46353.1"
                     /translation="MPLVYQTPGTRPCVVDPPLVLAPMAGITDGTYRLLLRRMGGVGL
                     VTMEFISSEGLTRGARRTERLMRWEPEERPLSIQIYGSDAARMAEAARRVEEEGVDVC
                     DINMGCPANKILKG"
BASE COUNT          249 a          586 c          578 g          218 t
ORIGIN      
        1 gccaaccggc gcgggatcac gaagtaccac cgcgagtcgg acgccgtctt cgcgggcaac
       61 gcctgcgcga tctggcgcta cgccgacgac cccgaggtgc tcaattcccc gacgacgacc
      121 tactggatgg ccccggccct gggcggcgtc gtgatccgga aggtccggac cgtcccgaag
      181 gacgacggga ccgaggaggt cacctacttc gagctgacgc acctccgggc cggcgtcgac
      241 ccggaggggt tccgggtccc cgaggggttc cggaaggaag gcacgggcgg ccgctgacgg
      301 ggggcgcccg gccccgggcg tcgttctgct aaattccacg cttccgggtc gacacggaac
      361 agaggtcgcc ctccaaggag acgtttcgat gaagccgtat gccgcgatcc tcgcgtccgc
      421 cctcatcgca gccctgccgc tcgcggctca gacgccggtc gacccgaagt cgccgattcc
      481 ggggttcccg gacggccgcg ggccgaaggg gcccgtgtcg atcaagctga tctcccccgc
      541 ggccgacgag gtgattccga tgcccgcggc cgagccgggc aagcccgagc cgaagggggc
      601 ccgggtcgag gtgaaggtcg cggtcgagaa ctacgagatc ttcaaggacg aggcgaccgg
      661 gaaggggcag cacgtccagc tcgtcctcga cgggcgcgcg cttccgaact ggtacgcgat
      721 cgacaaggcg tggatcttcc cggcgctgcc gaaggggacg cacacgctgc tggcgttcct
      781 ccagcgtccc tggaacgaga cgatccggga ggcgggcgcg ttcgcggccg tgacgttcca
      841 cgtcggcgag aagaacggca agccggcctt cgacccggcg cagccgtcga tcacggtcgc
      901 cgccccgcgc gggaagttcg agggcgagca ggccacgaag atcctcttcg acgtcttcgt
      961 caccggctgc accgtcggac cggcgaccga tcccgaggcg tgccagatca tctaccgcct
     1021 gaaccaggac ccgcagcagg tgctgacgga gtggaagccg gtcttctggg agaacgtccc
     1081 tcccggcaag cacagcttca tcgccgccct ctacaagggc gagaagcgga tcgaagcccc
     1141 gttcgccctg acgcagggct tcttcgaggt cgtggcggcc cccgctccgg ccgcggctcc
     1201 ggccgccgcc ccggcggcgc cggccactcc ggccggctga tcccggtccc gggcgcgggg
     1261 ccctccgccc cgcgcccgct cccgatgcct ctcgtctacc agacccccgg gacgcgcccc
     1321 tgcgtcgtcg atccgccgct cgtcctggcg ccgatggcgg ggatcaccga cggcacctac
     1381 cggctgctcc ttcgccggat gggaggcgtc ggcctcgtga cgatggagtt catctcgtcg
     1441 gaagggctga cgcgcggcgc ccgccggacg gagcggctga tgcgctggga gccggaggag
     1501 cgccccctct cgatccagat ctacggctcc gacgcggccc ggatggccga ggcggcacgc
     1561 cgggtcgagg aggagggggt cgacgtctgc gacatcaaca tgggctgccc ggcgaacaag
     1621 atcctgaagg g
//
LOCUS       DAOTEZ010000671         1626 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_70393_length_1626_cov_5.276257, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000671 DAOTEZ010000000
VERSION     DAOTEZ010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70393_length_1626_cov_5.276257"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="P5164_20650"
     CDS             <1..257
                     /locus_tag="P5164_20650"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882083.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRY46354.1"
                     /translation="EIRGKVPRVIKPPAGEAYAAVEAPKGELGFHLVASGGNKPYRLH
                     VRPPSFINLQALPEMAQGHLVGDLVAVIGTIDIVLGEVDR"
     gene            265..1431
                     /gene="nuoH"
                     /locus_tag="P5164_20655"
     CDS             265..1431
                     /gene="nuoH"
                     /locus_tag="P5164_20655"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HRY46355.1"
                     /translation="MSESLVHNVLVPFVKGLLTVILVATAAGILTWIERRGLGRLQIR
                     KGPNRVGPAGFFQWIADVVKLVFKEDVVPSRAEKVIHLLGPLLLFMPAVVVLGLVPWG
                     PELTLFGVTTPLHAAADVNVALLLILAVSSIGVYGIILAGWSSNSKYPLLGGLRSAAQ
                     LISYEVPMGFAVVAAVLMAGSLSMVEIIEAQKEAGVWFVFPGLVAFFLYFVSGVAETN
                     RTPFDLPEAESELVGGFHTEYSGFRWAIFFMSEYGNMISIGAVATTLFFGGWLRPFPN
                     VAWLSFLDVVPAFFWFAGKVFLFLLVYIWFRATFPRYRFDQLMALGWKALIPISLGNL
                     FLVALAALWGAAGLKVLGAVLVLLVAAGLVAAWRRPARASVTTRMAAEKVEVRA"
     gene            1428..>1626
                     /locus_tag="P5164_20660"
     CDS             1428..>1626
                     /locus_tag="P5164_20660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRY46356.1"
                     /translation="MSFLDRVLLLDIFAGLRVTARHAGRKADTIEYPEQSKEPSERFR
                     GMFRLDEERCIKCTLCAIECPI"
BASE COUNT          204 a          601 c          529 g          292 t
ORIGIN      
        1 cggagatccg agggaaggtc ccgcgggtca tcaagccgcc ggcgggcgag gcctacgccg
       61 ccgtcgaagc gccgaagggg gagctcggct tccacctcgt cgcgagcggc gggaacaagc
      121 cgtaccgcct ccacgtgagg cccccgagct tcatcaacct gcaagccctc cccgagatgg
      181 cgcagggcca cctcgtgggc gacctcgtgg ccgtcatcgg gacgatcgac atcgtcctcg
      241 gcgaagtcga ccggtaggaa cgcgatgtcc gagagcctcg tccacaacgt cctcgtcccc
      301 ttcgtgaagg ggctcctgac cgtcatcctg gtcgcgaccg cggccgggat cctgacctgg
      361 atcgagcgcc gcggcctcgg ccggctccag atccggaagg gtccgaaccg cgtcgggccc
      421 gccggcttct tccagtggat cgccgacgtc gtgaagctcg tcttcaagga ggacgtcgtc
      481 ccgtcccggg ccgagaaggt gatccacctc ctcgggccgc tcctcctctt catgcccgcg
      541 gtcgtcgtcc tcggcctcgt cccctggggc ccggagctga ccctcttcgg cgtgacgacc
      601 ccgctccacg ccgcggcgga cgtgaacgtc gcgctcctcc tgatcctcgc ggtctcctcg
      661 atcggcgtct acggaatcat cctcgccggc tggtcgtcga actcgaagta cccgctcctc
      721 ggcggcctcc gctcggcggc gcagctcatc agctacgagg tcccgatggg cttcgcggtc
      781 gtcgccgcgg tcctgatggc ggggtcgctc tcgatggtgg agatcatcga ggcgcagaag
      841 gaggccgggg tctggttcgt cttcccgggc ctcgtcgcct tcttcctcta cttcgtctcc
      901 ggcgtcgccg agacgaaccg gacgccgttc gacctccccg aggccgagag cgagctcgtc
      961 ggcgggttcc acaccgagta ctcgggcttc cgctgggcga tcttcttcat gtccgagtac
     1021 gggaacatga tctcgatcgg cgcggtcgcc acgacgctct tcttcggcgg ctggctccgc
     1081 cccttcccga acgtcgcctg gctctcgttc ctcgacgtcg tccccgcgtt cttctggttc
     1141 gcggggaagg tcttcctctt cctcctcgtc tacatctggt tccgggcgac gttcccgcgc
     1201 taccgcttcg accagctcat ggcgctcggc tggaaggcgc tcatcccgat ctcgctcggg
     1261 aacctcttcc tcgtcgcgct cgcggccctc tggggagccg ccgggctgaa ggtcctcggg
     1321 gcggtcctcg tcctcctcgt cgccgcgggc ctcgtcgcgg cctggaggcg cccggcccgg
     1381 gcgtccgtca cgacgcggat ggccgcggag aaggtggagg tgcgcgcgtg agcttcctcg
     1441 accgcgtcct cctcctcgac atcttcgcgg ggctgcgcgt cacggcccgg cacgcgggcc
     1501 gcaaggccga caccatcgag tacccggagc agagcaagga gccctcggag cgcttccggg
     1561 ggatgttccg cctcgacgag gagcgctgca tcaagtgcac gctctgcgcg atcgagtgcc
     1621 cgatca
//
LOCUS       DAOTEZ010000672         1616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_71178_length_1616_cov_5.187700, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000672 DAOTEZ010000000
VERSION     DAOTEZ010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71178_length_1616_cov_5.187700"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="P5164_20665"
     CDS             <1..342
                     /locus_tag="P5164_20665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46357.1"
                     /translation="GLAAKVELPENTAAFLLAEVRGRVVLELLSGPSAATYVFRGARE
                     EAGADLAALHFRRRGLALTRAEEAGPSGRPYRLALRRLAPLMRLRAATLARVVHGARW
                     TESLAKVLDAS"
     gene            465..626
                     /locus_tag="P5164_20670"
     CDS             465..626
                     /locus_tag="P5164_20670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46358.1"
                     /translation="MERSAAPRSLASTLVAAAACPTASPFAMSMSMPMCGSSLLSQEE
                     SGHAAGRRM"
     gene            729..>1616
                     /locus_tag="P5164_20675"
     CDS             729..>1616
                     /locus_tag="P5164_20675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014369910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRY46359.1"
                     /translation="MSTTANAARPALRPGTLAIHAGQESADPATNARAVPIYATTSYV
                     FDDPDHAADLFGLRKPGNIYTRIMNPTTDVFEKRVAALEGGVAAVATSSGQAAETLAI
                     LNLARAGDTIVSTTSLYGGTWALFAHTLPRLGVTARFVDASGPGGAARVAGAIDGTTK
                     AVYVETIGNPRLDVPDLGALADAAHSAGVPLVVDNTFATPLLCRPLEHGADVVIHSAT
                     KWIGGHGTAIGGIVVDGGRFDWSASERFRPFFVDPEPAYHGLSFSALAPAAYAARLRT
                     VLLRDVGVAVSPFNSFLFLQ"
BASE COUNT          200 a          669 c          526 g          221 t
ORIGIN      
        1 ggtctcgcgg cgaaggttga gctcccggag aacacggcag ccttcctcct cgccgaggta
       61 aggggccgcg tcgtcctcga gctcctctcg ggcccctccg ccgcgaccta cgtcttccgc
      121 ggggcgcgcg aggaggccgg ggccgacctc gcggcgctgc acttccgaag gcgcggcctc
      181 gccctcaccc gggccgagga ggccggtccc tcggggcgcc cctaccgcct cgcgctccgg
      241 cggctcgcgc cgctgatgcg gcttcgcgcg gccactctcg cgcgcgtcgt ccacggcgca
      301 cgctggacgg agagcctcgc aaaggttctc gacgcgagct gacgagggcc ggccggcgtg
      361 gcgtcgtgcc ggcccgtgcg gctgggcgac cgggtcacgg ccgcacgggc tcctccggtt
      421 ttcgcttgac aacgccccgg gcccctctcg taccctgccg cgccatggaa cgctccgccg
      481 ccccgcgctc gctcgcgagc acgctcgtcg cggcggccgc gtgcccgacg gcctcccctt
      541 tcgccatgtc catgtcgatg cccatgtgcg gctcttccct gctctcgcag gaggagtcgg
      601 ggcacgcggc gggacggcga atgtgacgtc cgcagcgtaa cgagccgaac gacggcaccc
      661 cgaccggccc tctcgcgaga gagggccggt ctttctttgc gcgcccgccg agaacgaaag
      721 gagaaccgat gtccacgacc gccaacgccg cccgccctgc actccgcccc gggaccctcg
      781 cgatccacgc gggccaggag agcgccgacc ccgcgacgaa cgcccgcgcc gtcccgatct
      841 acgcgacgac gtcgtacgtc ttcgacgacc ccgaccacgc ggccgacctc ttcggcctga
      901 ggaagccggg gaacatctac acgcggatca tgaacccgac gacggacgtc ttcgagaagc
      961 gcgtcgccgc gctcgaggga ggcgtcgccg ccgtcgcgac gtcgagcggc caggccgccg
     1021 agacgctcgc gatcctgaac ctcgcccgcg ccggcgacac gatcgtctcg acgacgtcgc
     1081 tctacggcgg cacctgggcc ctcttcgccc acacgctccc ccgcctcggc gtcacggccc
     1141 gcttcgtcga cgcctccggc cccggcggcg ccgcgcgcgt cgcgggggcg atcgacggga
     1201 cgacgaaggc cgtctacgtc gagaccatcg gcaacccgcg cctcgacgtg ccggacctgg
     1261 gcgccctcgc cgacgccgcc cactcggcgg gggtcccgct cgtcgtcgac aacaccttcg
     1321 cgacgcccct cctctgccgc cccctcgagc acggcgccga cgtcgtgatc cactcggcga
     1381 cgaaatggat cggcgggcac gggacggcga tcggcgggat cgtcgtcgac ggaggccgct
     1441 tcgactggag cgcgtccgaa cgcttccgcc ccttcttcgt cgaccccgag ccggcctacc
     1501 acggcctctc cttctcggcc ctcgccccgg cggcctacgc ggcccgcctc cggaccgtcc
     1561 tcctccgcga cgtcggcgtc gcggtctccc ccttcaactc gttcctcttc ctccag
//
LOCUS       DAOTEZ010000673         1615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_71245_length_1615_cov_4.427564, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000673 DAOTEZ010000000
VERSION     DAOTEZ010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71245_length_1615_cov_4.427564"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="P5164_20680"
     CDS             complement(<1..330)
                     /locus_tag="P5164_20680"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006891760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HRY46360.1"
                     /translation="MLFDKSKLRLPDTEDALEGRPTPLSVPNRHFVNGREILPPFPAG
                     LERAVFGMGCFWGAEKAFWALPGVYTTAVGYSGGLTPNPTYKEVCTGRTGHAEVVLVV
                     FDPAAVAF"
     gene            complement(387..1442)
                     /locus_tag="P5164_20685"
     CDS             complement(387..1442)
                     /locus_tag="P5164_20685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HRY46361.1"
                     /translation="MPADRPWTEAALERGLVPDVLVRRRIRELLRQRLAEERAADAEA
                     ALEREERLVAEMRESPIALATAEANEQHYEVPPAFFELVLGAHRKYSSGLWSEGVTTL
                     DAAEAAMLALTCARARLENGQNVLELGCGWGSLTLWMAERYPASRIVAVSNSRSQREY
                     VEARAAERGLSNVKVVTCDMNVFDPATGPAFDRVVSVEMFEHMRNWPELLGRIASWLK
                     PDGRLFVHVFSHRDLSYPFVARDESDWMARTFFTGGLMPSDHLLLRFPDHLLVEERWR
                     VNGTHYARTAEAWLANLDRDRKAVRSLFSGTWGPGVARRRVAEWRVFLMACAELWAFG
                     GGNEWMVSHYRLRRRFA"
     gene            complement(1432..>1615)
                     /locus_tag="P5164_20690"
     CDS             complement(1432..>1615)
                     /locus_tag="P5164_20690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46362.1"
                     /translation="AWALTAPLIGAAVHFAVRPALRAAARRLRPARATVVPPGPAPAG
                     ELSTVPVPAVEESDAR"
BASE COUNT          232 a          577 c          609 g          197 t
ORIGIN      
        1 gaacgcgacc gcggccgggt cgaagacgac gaggacgacc tcggcgtgcc cggtccgacc
       61 cgtgcacacc tccttgtacg tcgggtttgg cgtcagcccg cccgagtagc cgacggccgt
      121 cgtgtagacg ccggggagcg cccagaacgc cttctcggcg ccccagaagc agcccatccc
      181 gaacacggcc ctctcgaggc cggccgggaa aggcgggagg atctcgcgcc cgttcacgaa
      241 gtggcggttc gggacggaga gcggcgtcgg gcgcccctcc agcgcgtcct ccgtgtcggg
      301 aaggcggagc ttgctcttgt cgaaaagcac ggttcacctc cgggtcggtc gccccgaagt
      361 gtagccgggg cggcgcgccg cgctcctcag gcgaagcggc ggcggagccg gtagtgggag
      421 accatccact cgttcccgcc gccgaacgcc cagagctccg cgcaggccat caggaagacg
      481 cgccactccg ccacccgtcg ccgtgcgacg ccggggcccc aggtcccgga gaagagggag
      541 cggacggcct tccggtcccg gtcgaggttc gcgagccacg cctccgccgt ccgcgcgtag
      601 tgcgtgccgt tcacgcgcca gcgctcctcc acgaggaggt ggtcggggaa gcggaggagg
      661 aggtggtccg agggcatcag gccgcccgtg aagaaggtcc gcgccatcca gtcgctctcg
      721 tccctcgcga cgaacgggta cgagaggtcg cggtgggaga agacgtggac gaagagccgc
      781 ccgtcgggct tcagccagga ggcgatccgg ccgaggagct cgggccagtt gcgcatgtgc
      841 tcgaacatct ccacggagac gacccggtcg aaggccgggc cggtcgccgg gtcgaagacg
      901 ttcatgtcgc aggtgacgac cttgacgttc gaaaggcccc gctccgccgc ccgcgcctcg
      961 acgtattccc gctggctcct cgaattcgag acggcgacga tccgggaggc ggggtaacgc
     1021 tcggccatcc agagcgtgag cgagccccac ccgcagccga gctcgaggac gttctggccg
     1081 ttctcgagcc gggcgcgcgc gcaggtgagc gcgagcatcg cggcctcggc cgcgtcgagc
     1141 gtcgtcaccc cttccgacca gaggccggag gagtacttcc ggtgcgcgcc gaggacgagc
     1201 tcgaagaatg cgggcgggac ctcgtagtgc tgctcgttcg cctcggccgt cgcgagggcg
     1261 atcgggctct cgcgcatctc ggcgacgagc cgctcctcgc gctcgagcgc cgcctcggcg
     1321 tcggcggccc gctcctcggc gagccgctgc cggagcagct cccggatccg ccggcggacg
     1381 aggacgtccg ggacgaggcc ccgttccagg gcggcctccg tccagggacg gtcagcgggc
     1441 atcgctctcc tctacggccg ggaccggcac cgtcgagagc tcccccgcgg gcgccggccc
     1501 gggcgggacg accgtcgccc gggccggccg gagccggcgg gcggcggcgc gcagggcggg
     1561 gcgcaccgcg aagtggacgg cggcgccgat caggggggcg gtcagggccc aggca
//
LOCUS       DAOTEZ010000674         1612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_71475_length_1612_cov_7.455363, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000674 DAOTEZ010000000
VERSION     DAOTEZ010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71475_length_1612_cov_7.455363"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="P5164_20695"
     CDS             <1..561
                     /locus_tag="P5164_20695"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HRY46363.1"
                     /translation="LVNTVTVTAPAGTTDPTPDDNTAIDTDTAVPSADLVVTKVASSG
                     VVSVGSELEFRITVRNLGPSTATDVVLTDPLPSRLLFVAAETSQGSCSGTATVTCALG
                     TLAPDSQAEVVLTVRALSPGAFANTATATLAEPDPTPPNNVSTAPLEVQGQAEVPTTS
                     ETGLLALALLIAGAGILLLRRTLPVA"
     gene            complement(771..>1612)
                     /locus_tag="P5164_20700"
     CDS             complement(771..>1612)
                     /locus_tag="P5164_20700"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HRY46364.1"
                     /translation="LNPADWKVVEGKLPFLFLPKRPYVPGCEAAGVVESLDPRVSGLR
                     EGDEVVCSTGFGGALAEKVAVSALQVVRKPAALSFEEAAAIPAAGMSALQSLRDLGGV
                     RAGHRLLVNCAGGGVGVFAVQIGKILGAHVTAVASAGKHERLAGLGADVLVDYRKDDF
                     TRAREEWDVVFDVVPNRSFAACSRVLSPEGVYVTTLPGPGPFFASVAAALRAPFGFRK
                     RCRALMLKPRPEDLGWLLDRAAEGSLKAVVGEVVPFERAAGALSDLSAGHVTGKTVVK
                     IAD"
BASE COUNT          235 a          614 c          551 g          212 t
ORIGIN      
        1 ctcgtcaaca ccgtcacggt cacggcgccg gcgggaacga ccgacccgac gccggacgac
       61 aacacggcga tcgacaccga caccgccgtt ccgagcgcgg atctcgttgt cacaaaggtc
      121 gcctcttcgg gcgtggtctc cgtcggctcc gagctcgagt tccgaatcac ggtccgcaac
      181 ctcggcccga gcaccgcgac ggatgtcgtt ctcaccgacc cgctcccgag caggctcctc
      241 ttcgtggccg cggagacgtc ccagggctcc tgctccggga ccgcgacggt gacctgcgcg
      301 ctcggaacgc tcgctccgga ttcgcaggcc gaggtcgtcc tgacggtccg cgccctttcg
      361 cccggagcct tcgcgaacac ggccaccgcg acgctggccg agcccgatcc cacccctccg
      421 aacaacgtct ccacggcgcc gctcgaggtc cagggccagg ccgaggtccc caccacttcg
      481 gagacggggc tcctcgcgct cgctctgctg attgcgggag cagggatcct cctccttcgg
      541 aggaccctcc ccgtcgcctg acccctccga cgtcgattgc cctggaagga gcgcccgtgg
      601 ggcgcccctt ccagggctcg agcgggtccg ggccggtcgc cggcagccgg gggggcacgg
      661 ccccggccag cgccatccgg cgccaggctc ccgccgcctc cccgtccgcg tcgtcggctc
      721 cggcgagagg ccggcctccc cggtcacggc aggccggtca ttgggtcggt tcagtcggcg
      781 atcttcacga ccgtcttccc ggtgacgtgc ccggccgaga ggtccgagag ggctcccgcg
      841 gccctctcga acggaaccac ctccccgacg acggccttca ggctcccctc cgcggcgcgg
      901 tcgaggagcc agccgaggtc ctcgggccgc ggcttcagca tcagggcgcg gcacctcttc
      961 cggaagccga acggcgcgcg gagcgcggcg gcgaccgagg cgaagaacgg accggggccg
     1021 ggaagcgtcg tgacgtagac gccctcgggt gagaggacgc gggagcacgc cgcgaagctc
     1081 cggttcggga cgacgtcgaa gaccacgtcc cactcctcgc gtgctctcgt gaagtcgtcc
     1141 ttccgatagt cgacgaggac gtcggcgccg aggccggcga ggcgctcgtg cttccccgcg
     1201 ctcgcgacgg cagtgacgtg ggcaccaagg atcttcccga tctggaccgc gaagacgccg
     1261 actcccccgc ccgcgcagtt cacgagcagt cgatgccctg cgcggacccc gccgaggtcg
     1321 cggagcgact ggagcgccga cattcccgcg gccgggatcg ccgccgcctc ttcgaaggag
     1381 agcgcagcgg gcttccggac gacctgaagg gccgagacgg cgaccttctc ggcgagagcg
     1441 ccgccgaagc cggtcgagca gacgacctcg tcgccttcgc ggagaccgga gacgcgtggg
     1501 tcgagcgact cgacgacgcc ggccgcctca cagccgggaa cgtacggccg cttcggaagg
     1561 aagaggaacg ggagcttccc ctcgacgacc ttccagtcgg ccgggttgag cg
//
LOCUS       DAOTEZ010000675         1607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_71874_length_1607_cov_6.057990, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000675 DAOTEZ010000000
VERSION     DAOTEZ010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71874_length_1607_cov_6.057990"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="P5164_20705"
     CDS             complement(<1..871)
                     /locus_tag="P5164_20705"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRY46365.1"
                     /translation="MNLFRTKPIDQLVAEGADTGKGLKRELTAVDLVALGIGAIIGAG
                     IFATLGTATSGGGSLPPAGPGIVVSILLTALACGFCGLCYAEFASLVPVAGSAYTYSY
                     ATLGELVAWIIGWDLILEYAVGNIAVAISWAAYFRQLLLGFGLEFPAFLATDYRSTLL
                     AAKAAASGGVGALSPEQAVAYQAHLHHPELFGVPIVFNLLAALITAAITILLVRGIKE
                     SARVNDFIVFLKVATLLFFVAVGAFFVKPANWHPFFPGGFSGVWMGASLMFFAYIGFD
                     AISTAAEECKDPGR"
     gene            complement(907..>1607)
                     /locus_tag="P5164_20710"
     CDS             complement(907..>1607)
                     /locus_tag="P5164_20710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRY46366.1"
                     /translation="KRTKAVFLDAGNTLLAADPPVEAVYGEAFRRWGLSAGDGAVHDA
                     LRATWAHVAARQARGEERWGGGGGERAFWQDFVQDVWGRLGGGLLPEGLLDDLIVHFA
                     RQESWVLYPEVLEVLAALRERGLKLVVVSNWDSTLPALLDRLDLTRYFDGVVVSALVG
                     ASKPARAIFDTALAAVDVAAHEALHVGDSPTEDYEGARSAGLPALLLDRAGVAGDGYE
                     TIRTLQEILPRIAP"
BASE COUNT          268 a          555 c          583 g          201 t
ORIGIN      
        1 cgcggccggg gtccttgcat tcctcggccg ccgtcgaaat cgcgtcgaac ccgatgtagg
       61 cgaagaacat cagggaggcg cccatccaga ccccggagaa cccgcccggg aagaacgggt
      121 gccagttcgc cggcttgacg aagaaggcgc cgacggcgac gaagaagagg agcgtcgcga
      181 ccttcaggaa gacgatgaag tcgttcacgc gcgccgattc cttgatcccg cggacgagga
      241 ggatcgtgat cgccgccgtg atgagcgccg cgaggaggtt gaagacgatc gggaccccga
      301 agagctccgg gtggtggagg tgagcctggt acgcgaccgc ctgctcgggc gagagcgcgc
      361 cgacgccgcc cgaggccgcg gcctttgccg cgaggagggt cgaccggtag tcggtcgcga
      421 gaaaggccgg gaactccagg ccgaagccca ggaggagctg tcggaagtag gcggcccacg
      481 agatcgccac ggcgatgttc ccgacggcgt actcgaggat caggtcccac ccgatgatcc
      541 acgcgacgag ctcgccgagg gtcgcgtagc tgtaggtgta ggccgacccg gcgaccggga
      601 cgaggctcgc gaactccgcg tagcagaggc cgcagaagcc gcaggcgagg gccgtcagga
      661 ggatcgagac gacgatgccg gggcccgccg gggggagcga gccgcccccc gaggtcgccg
      721 tcccgagcgt cgcgaagatg cccgcgccga tgatcgcccc gatcccgagg gccacgaggt
      781 cgacggcggt cagctcgcgc ttcagccctt ttccggtgtc ggccccctcg gcgacgagct
      841 ggtcgatcgg cttcgtacgg aagaggttca ttcgcggctc ctcgtcggtt cgcgttcggc
      901 gcggcgtcag ggggcgatcc ggggaaggat ctcctggagc gtccggatcg tctcgtaccc
      961 gtcgcccgcg acgccggccc ggtcgagaag gagggccgga aggccggcgg agcgcgcccc
     1021 ctcgtagtcc tccgtcggag agtcgccgac gtggagcgcc tcgtgagcgg cgacgtcgac
     1081 ggcggccagc gccgtgtcga agatcgcgcg ggccggcttg ctcgctccga cgagcgccga
     1141 gacgacgacg ccgtcgaagt agcgagtcag gtcgagccgg tcaaggaggg cgggaagcgt
     1201 cgaatcccag ttgctgacga cgacgagctt cagccccctc tcgcggaggg ccgcgagcac
     1261 ctcgagcacc tccggataga ggacccagct ctcctgccgg gcgaaatgga cgatcaggtc
     1321 gtcgagaagg ccctcgggga gaaggccgcc gccgagccgc ccccagacgt cctggacaaa
     1381 atcctgccag aacgccctct ctcctccgcc cccgccccag cgctcctccc cacgggcctg
     1441 gcgcgccgcg acgtgcgccc acgtcgcccg cagggcatcg tgcacggcgc cgtcgccagc
     1501 cgacaggccc cacctccgga acgcttcgcc gtagaccgcc tccaccggcg ggtcggccgc
     1561 gaggagcgtg ttgccggcgt cgaggaagac cgccttcgtc cgcttca
//
LOCUS       DAOTEZ010000676         1605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_72038_length_1605_cov_5.882581, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000676 DAOTEZ010000000
VERSION     DAOTEZ010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72038_length_1605_cov_5.882581"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="P5164_20715"
     CDS             <1..962
                     /locus_tag="P5164_20715"
                     /inference="COORDINATES: protein motif:HMM:NF015043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HRY46367.1"
                     /translation="APGLTFYAAHNKVDRETGEWLHFGLEFGRKMRVHLTTFAADGSL
                     ANHDSWPLPRFSYLHDWFVTRRHVVLHLHPATIEVFGLLLGLRSIVDSLRWKGADGSL
                     LMILPRDRSGGAEPRFVETEAAFMWHSFNGHEEGDELVLDFVGYDDPDHFIGDEPLAL
                     EVMKGRPGRFEHPGSVRRIRVAPGAARARNEVLAPGNFEWPRVDDRLLGRRQRWGYAA
                     SAREKLFFWNAVARVDFENGHVERFDFGDRVFTSEPVFVPDPERSSEEAGWLLVELYD
                     GDTRKSSLAVLDASRIGDGPLARVLLEHHVPISFHGFWKGAPA"
     gene            1046..>1605
                     /locus_tag="P5164_20720"
     CDS             1046..>1605
                     /locus_tag="P5164_20720"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY46368.1"
                     /translation="MLVVGAGAAGLAAARTLADRGLSPTVVDKARGVGGRIATRRLSD
                     GLPYDHGATLFEPVTDAFRRRLLSWEAEGLVERIGVGEETFRARGGATALARSLASGL
                     DVRLGAKATALAHDGAAFRLDLEEGEPLRAGVVLLTPPVPQSLDLLAQGGLAERLPAG
                     LAAGLRRVAYAAGVVLLLRLDRRVDGL"
BASE COUNT          194 a          580 c          603 g          228 t
ORIGIN      
        1 tcgcgcccgg cctcacgttc tacgccgcgc acaacaaggt ggaccgggag acgggggagt
       61 ggctccactt cggcctcgag ttcgggcgga agatgcgggt ccacctgacg acgttcgccg
      121 ccgacggctc gctcgcgaac cacgattcgt ggccgctccc gcgcttctcc tacctgcacg
      181 actggttcgt gacgcgccgc cacgtcgtcc ttcacctcca cccggcgacg atcgaggtct
      241 tcggcctcct cctcggcctc cggagcatcg tcgactcgct ccggtggaag ggcgcggacg
      301 ggagcctcct gatgatcctg ccgcgggaca ggagcggggg cgcggagccg cgcttcgtcg
      361 agaccgaggc ggcgttcatg tggcactcgt tcaacgggca cgaggagggg gacgagctcg
      421 tcctcgactt cgtcggctac gacgaccccg accacttcat cggcgacgag ccgctcgcgc
      481 tcgaggtgat gaaggggcgc ccgggccggt tcgagcaccc gggctcggtc cggcgcattc
      541 gcgtggcgcc gggagcggcg cgcgcgcgga acgaggtgct cgcccccggc aacttcgagt
      601 ggccgcgggt cgacgaccgg ctcctcggcc gccgccagcg atggggatac gccgcgagcg
      661 cccgggagaa gctcttcttc tggaacgcgg tcgcccgcgt cgacttcgag aacgggcacg
      721 tcgagcggtt cgacttcggc gaccgcgtct tcacgagcga gccggtcttc gtccccgacc
      781 cggagcggtc gtcggaggag gcgggctggc tcctcgtgga gctctacgac ggtgacacgc
      841 ggaagagctc cctcgccgtc ctggacgctt cgcggatcgg cgacgggccg ctcgcccgcg
      901 tcctcctcga gcaccacgtc ccgatcagct tccacggctt ctggaagggg gcgccggcct
      961 gagagggtgc gtccgccggg gccgcgccgg cgtagagaca cggatggagc ggcttggaag
     1021 gacgtgtgcc cccgggtcgg gtcccgtgct cgtcgtcggg gccggggcgg cggggctcgc
     1081 cgccgcgcgg acgctggccg atcgcggcct gtccccgacc gtcgtcgaca aggcgcgcgg
     1141 cgtcggcggg cggatcgcga cgcgacggct cagcgacggc ctgccgtacg accacggcgc
     1201 gaccctcttc gagcccgtca cggacgcgtt ccggaggagg ctcctctcgt gggaggcgga
     1261 ggggctcgtc gagcggatcg gtgtcggcga agagacgttc cgggctcgcg ggggcgcgac
     1321 ggcgctggcg cggagcctcg ccagcgggct ggacgtccgc ctcggggcga aagcgaccgc
     1381 cctcgcccac gacggagcgg cgtttcgcct cgacctggag gagggagagc ctctccgcgc
     1441 cggggtggtc ctcctgacgc cacccgttcc gcagtcgctc gacctcctcg cacagggcgg
     1501 gctcgcggag aggctccccg cggggctggc cgccgggctc cggcgcgtcg cgtacgccgc
     1561 cggggtcgtc ctcctcctcc gcctcgaccg gcgcgtggac ggcct
//
LOCUS       DAOTEZ010000677         1601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_72327_length_1601_cov_5.510349, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000677 DAOTEZ010000000
VERSION     DAOTEZ010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72327_length_1601_cov_5.510349"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="P5164_20725"
     CDS             complement(<1..663)
                     /locus_tag="P5164_20725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46369.1"
                     /translation="MPSADRHLGELELLERAMTPGLAPDPHAASCPTCRDRIEGLAAG
                     REHLADLAGLAAAEESGARVPVPAALSRLSRTLARAEDLVRAAERDDAALPAWLDEAS
                     ARPDFPDVALHALQIASRLTSRRPSVALAFARSIRQRLHGDVGGDGTLLAAEIGLLES
                     QAFVYVGRIDEACERALAASSAIEAAAAPALLRARADYYAGSALWGAGRSEAALSRLL
                     AAR"
     gene            complement(650..1210)
                     /locus_tag="P5164_20730"
     CDS             complement(650..1210)
                     /locus_tag="P5164_20730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46370.1"
                     /translation="MPGETAPLENVVWAASRFAPSQDGARPAGLEPRYWDWEDLYSRL
                     GPDLVVLGARRFGLSRDDAEEALQMAATAIVLAAPSVRSPEAYLTSVFLRECLGILRR
                     RSQLARTEVAPPVGFDAADDSCERIEVTCRFRQAFSLLSPVCRSMMRSCLLDGRRRAE
                     SDSSSAARTAYKRYRKCLRTLAHALG"
     gene            complement(1218..>1601)
                     /locus_tag="P5164_20735"
     CDS             complement(1218..>1601)
                     /locus_tag="P5164_20735"
                     /inference="COORDINATES: protein motif:HMM:NF024265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE-like domain-containing protein"
                     /protein_id="HRY46371.1"
                     /translation="DPFVLDFLGSVNIFHGRVENGRAHLGPLSVAYQGAPHDGALPAS
                     GYARSWEIDLSRDLGGADGFPAAVRHVAAAGAVVRLELCTDDGTTLKVEISRERFSEI
                     APSPGDRLRVVPRNVRVFLGGPGRG"
BASE COUNT          234 a          567 c          617 g          183 t
ORIGIN      
        1 gcgggccgcg aggaggcggc tcaacgcggc ctcggatcga cccgcccccc agagggccga
       61 gccggcgtag tagtcggcgc gggcgcgcag cagggcgggg gcggccgccg cctcgatcgc
      121 cgaagaggcc gcgagcgccc gctcgcacgc ctcgtcgatc cgtcccacgt agacgaaggc
      181 ctgactctcc aggaggccga tctcggccgc gagaagcgtc ccgtcccctc caacgtctcc
      241 atgcagacgc tggcggatcg accgggcgaa cgcgagcgcc accgaggggc gccgggacgt
      301 gagcctcgag gcgatctgga gcgcgtgcag cgccacgtcc gggaagtcgg ggcgagcgga
      361 tgcctcgtcg agccaggccg ggagagcggc gtcgtcgcgt tccgccgcgc ggacgaggtc
      421 ctccgcccgg gcgagcgtcc gggacaggcg ggagagcgcc gcggggacgg gaactcgggc
      481 gcctgactcc tccgcggccg cgagtccggc gaggtcggcc aggtgctcgc gacccgccgc
      541 gagcccctcg atccggtccc ggcaggtcgg gcagctcgcg gcgtgcgggt cgggcgcaag
      601 gccgggcgtc atcgcccgct ccagcagctc gagctctccg aggtgccgat cagccgaggg
      661 catgggcgag cgtcctcagg catttccggt agcgcttgta ggccgtgcgg gcggcggacg
      721 aggaatcgga ttcggcgcgc ctcctcccgt cgagcaggca gctccgcatc atcgaccggc
      781 agacgggaga aaggagcgag aacgcctgcc ggaaccggca ggtgacctcg atccgctcgc
      841 aggagtcgtc ggccgcgtcg aaaccgaccg gcggggcgac ctcggtccgg gccagctggc
      901 tccgccgtcg gaggatcccg aggcattcgc ggaggaagac cgacgtgagg taggcctcgg
      961 gcgaccggac ggacggagcg gccaggacga tggccgtcgc cgccatctgg agcgcctcct
     1021 ccgcgtcgtc ccggctgagc ccgaaccggc gcgcgccgag gacgacgagg tccggtccga
     1081 ggcgggagta gaggtcctcc cagtcccagt agcgcggctc gagcccggcc ggacgtgccc
     1141 catcctgcga cggcgcgaat cgcgacgccg cccagaccac gttctcgagc ggtgcggtct
     1201 ctccaggcac ggggcgatta tcctcgcccc ggcccgccga ggaaaaccct cacgtttcgg
     1261 ggtacgacac ggaggcggtc tcccggggag ggcgcgatct ccgagaagcg ctccctcgag
     1321 atctcgacct tgagcgtcgt cccgtcgtcc gtgcagagct cgagccggac gacggctccg
     1381 gctgccgcga cgtgacggac tgcagccggg aagccgtccg ccccgcccag gtcccgcgag
     1441 aggtcgatct cccaggaccg cgcgtagccg gacgccggga gcgcgccgtc gtggggggcg
     1501 ccctggtagg cgacggagag cgggccgagg tgggcccggc cgttctcgac ccggccgtgg
     1561 aagatgttca cggagccgag gaagtcgagg acgaacgggt c
//
LOCUS       DAOTEZ010000678         1597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_72634_length_1597_cov_7.245136, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000678 DAOTEZ010000000
VERSION     DAOTEZ010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72634_length_1597_cov_7.245136"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="P5164_20740"
     CDS             complement(<1..1027)
                     /locus_tag="P5164_20740"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRY46372.1"
                     /translation="MAEIRSRGAADPFRGLEYLPEPDYARPLRGLTPELAARVRRRLD
                     LLYGEEAGEHVLRHVDRLVKVHDAHATPEIRAAEAAFDPRERFSEKDAVLITYGDLVV
                     SEGRSPLATLSDFAYVLFRGLVTTVHVLPFFPSSSDRGFSIISYEEVDPGLGSWEEIA
                     RLGRGFRLMFDGVFNHVSAKSRRFQKFLAGDPEYARFFRVFSSREEVDDDHLKLVLRP
                     RTSDLLSEFATVEGPRWLWTTFSRDQIDLNFRHPRVLLEVLEILLYYVRRGADLVRLD
                     AVTYIWHELGTQCAHLRQTHEVVKLVRDVLDAAAPHVALVTETNVPHADNVTYFGDGT
                     DEAQMVYN"
     gene            1132..>1597
                     /locus_tag="P5164_20745"
     CDS             1132..>1597
                     /locus_tag="P5164_20745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HRY46373.1"
                     /translation="MSDFAQSGPITTLTRLGTRPLVELEDALVRYTRRNKAALLIPSL
                     VSELERPALLRICDEIAQAPYLDTVLISLDRADEAGYRFALDYFKRLKRRTVVLWNDG
                     PGVKALLAKLEKADLVLGERGKGRACWIGFGYLLAQGNVEYIALHDADVLDYD"
BASE COUNT          253 a          549 c          563 g          232 t
ORIGIN      
        1 agttgtagac catctgcgcc tcgtccgtcc cgtcgccgaa gtaggtgacg ttgtccgcgt
       61 gggggacgtt cgtctccgtg acgagggcga cgtgcggagc ggcggcgtcg aggacgtccc
      121 gcacgagctt cacgacctcg tgcgtctggc ggaggtgcgc gcactgcgtc ccgagctcgt
      181 gccagatgta ggtcacggcg tcgaggcgga cgaggtccgc gccgcgcctc acgtagtaga
      241 ggaggatctc gaggacctcg aggaggaccc tcgggtggcg gaagttgagg tcgatctggt
      301 cgcgcgagaa ggtcgtccag agccagcgcg gcccctccac cgtcgcgaac tcgctcagga
      361 ggtcggaggt cctcggccgg aggacgagct tcaggtggtc gtcgtcgacc tcctcgcggg
      421 aggagaagac tcggaagaag cgcgcgtact ccgggtcccc cgcgaggaac ttctggaagc
      481 ggcggctctt ggcggaaacg tggttgaaga ctccgtcgaa catcagccgg aagccgcgcc
      541 cgaggcgcgc gatctcctcc cagctcccga gcccggggtc gacctcctcg tacgagatga
      601 tcgagaagcc gcggtcggag gacgagggga agaacggcag gacgtggacc gtcgtgacga
      661 ggccgcggaa gaggacgtag gcgaagtcgg agagcgtcgc gagaggcgag cgcccctccg
      721 agacgacgag gtcgccgtac gtgatcagga ccgcgtcctt ctcgctgaag cgctcgcgcg
      781 ggtcgaaggc cgcctcggcc gcgcgaatct ccggcgtcgc gtgggcgtcg tggaccttca
      841 cgagccggtc gacgtgccgg aggacgtgct cgccggcctc ctcgccgtag aggaggtcga
      901 gccgcctccg gacccgcgcg gcgagctccg gcgtcagccc ccgcaggggc cgcgcgtagt
      961 cgggctcggg aaggtactcg agcccgcgga acgggtccgc ggcgcccctg gaacggatct
     1021 ccgccatgag gggaccgtag cacgacccgc aggcgagact cgtcgcccgc cctgagatcg
     1081 aacggccgtt ttcgcttccc tccgcgcaac gggccggggt agaaagccct catgagcgac
     1141 ttcgcgcagt ccggcccgat cacgaccctc acccgtctcg gcacccggcc gctcgtcgag
     1201 ctggaggacg ccctcgtccg ctacacccgc aggaacaagg ccgcgcttct gatcccctcg
     1261 ctcgtctccg agctggagcg cccggcgctc ctcaggatct gcgacgagat cgcgcaggcc
     1321 ccctacctcg acaccgttct gatctccctc gaccgggccg acgaagccgg ctaccgcttt
     1381 gcgctcgatt acttcaagcg cctgaagcgg cggaccgtcg tcctctggaa cgacggcccg
     1441 ggcgtgaagg ctctcctcgc gaagctcgag aaggccgacc tcgtcctcgg cgagcgcggg
     1501 aaggggcgcg cctgctggat cggcttcggc tacctcctcg cgcaggggaa cgtcgagtac
     1561 atcgcgctcc acgacgccga cgtcctcgac tacgacc
//
LOCUS       DAOTEZ010000679         1596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_72718_length_1596_cov_6.062946, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000679 DAOTEZ010000000
VERSION     DAOTEZ010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72718_length_1596_cov_6.062946"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..498)
                     /locus_tag="P5164_20750"
     CDS             complement(34..498)
                     /locus_tag="P5164_20750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRY46374.1"
                     /translation="MKAPSRVRGYSLVELVVVVTIILTLTAMIVPVARFNWTRMKEME
                     LKEALRTMRTAIDEHKRLSDQGLIPVELDTEGYPKELEDLVKGIELVGQIKKTRKFLR
                     KVPIDPMTGEAEWGLRSLQDDFDSTSWGRQNVYDVYSLSERTALDGTKYKDW"
     gene            complement(506..>1596)
                     /locus_tag="P5164_20755"
     CDS             complement(506..>1596)
                     /locus_tag="P5164_20755"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46375.1"
                     /translation="VTSYQYQDVGIKVDVEARVHHNREVSLKLTVEISEVKPTAGEQP
                     TISTRTITSNIRLKDGETSFLAGLIRTKSANSRSGLPFLSDVPLIGSLFSGKRQETAR
                     DDIFLSLTPHITRAPQIDEQDLVPVWVGTENNVSFSGLNVRLESPQAPATPFDPPAEA
                     PASRPSAPTGPVPGTMPGRAILPVQGSGPNDPFRRTPAPAPRTGSNILSGDAAQRSAA
                     FVETEGPRPLALGLELENGELAVGETALLTLNGPPELADAGALEVTIEWDPAVAELVA
                     VTPGPWRTGSAALNTRLDSDRGPGRVRIGLGTPTGVVGLPSGALARFTLRALAPGDAL
                     VRVSAGAGLGKSGPLRPEANAVPLVVRD"
BASE COUNT          203 a          559 c          582 g          252 t
ORIGIN      
        1 ggggggcgag ggtctcttca ggaggcatcg tcgtcaccag tccttgtact tcgtcccgtc
       61 gagcgccgtc cgctcggaga gagagtagac gtcgtagacg ttctggcggc cccagctcgt
      121 cgagtcgaag tcgtcctgca ggctccggag cccccattcc gcctctcccg tcatcgggtc
      181 gatcgggacc ttccggagga acttgcgcgt cttcttgatc tgcccgacga gctcgatccc
      241 cttcacgagg tcctccagct ccttcgggta gccctccgtg tcgagctcga ccgggatgag
      301 cccctggtcg gagaggcgct tgtgctcgtc gatcgccgtg cgcatcgtcc ggagggcttc
      361 cttcagctcc atctccttca tccgcgtcca gttgaaccgc gccaccggca cgatcatcgc
      421 cgtcagcgtc aggatgatcg tgacgacgac gacgagctcg acgagcgagt agccgcggac
      481 gcgggacggg gccttcacgg cgtcctcagt ccctcacgac gagcggtacc gcgttcgcct
      541 cgggacggag cggtccgctc ttgccgagcc ccgcgccggc ggagaccctc acgagggcgt
      601 cgcccggggc gagcgcgcgg agcgtgaagc gggcgagcgc gccgctcggg aggccgacga
      661 cgcccgtcgg cgtgccgagg ccgatccgca cccgccccgg cccccggtcg gaatcgaggc
      721 gggtgttgag cgccgccgat ccggtccgcc acgggccggg cgtcaccgcg acgagctcgg
      781 ccacggccgg gtcccattcg atcgtcacct cgagcgcgcc ggcgtcggcg agctccgggg
      841 gaccgttgag cgtcaggagc gccgtctccc cgaccgccag ctcgccgttc tcgagctcga
      901 gcccgagggc caggggccgc ggcccctcgg tctcgacgaa ggccgccgag cgctgcgccg
      961 cgtcgcccga gaggatgttc gagccggtcc ggggcgcggg tgcgggcgtc cgccggaacg
     1021 ggtcgttcgg gccgctcccc tgcaccggga ggatcgcccg gcccggcatc gtccccggga
     1081 ccgggcccgt cggcgcggag ggacgcgagg cgggcgcctc cgcgggcggg tcgaacgggg
     1141 tggcgggcgc ctgaggcgac tcgagcctca cgttgaggcc ggagaaggag acgttgttct
     1201 ccgtcccgac ccagacgggg acgaggtcct gctcgtcgat ctgcggcgcg cgggtgatgt
     1261 ggggcgtgag gctcaggaag atgtcgtccc gggccgtctc ctgcctcttg ccgctgaaga
     1321 ggctcccgat gagggggacg tccgagagga acgggagccc ggaccgggag ttcgccgact
     1381 tcgtccggat cagcccggcg aggaagctcg tctcgccgtc cttcagccgg atgttcgacg
     1441 tgatcgtccg ggtgctgatc gtcggctgct ccccggccgt cggcttgacc tcggagatct
     1501 cgacggtcag cttcagcgag acctcccggt tgtggtggac gcgcgcctcg acgtcgacct
     1561 tgatgccgac gtcctggtac tggtacgagg tgaccg
//
LOCUS       DAOTEZ010000680         1591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_73062_length_1591_cov_5.686849, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000680 DAOTEZ010000000
VERSION     DAOTEZ010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73062_length_1591_cov_5.686849"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /gene="nadA"
                     /locus_tag="P5164_20760"
     CDS             complement(<1..239)
                     /gene="nadA"
                     /locus_tag="P5164_20760"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007102409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRY46376.1"
                     /translation="MASSPTPLPTLAIPLPAELPDAILRLKEERDALILAHYYQEPEI
                     QDLADFVGDSLELARKGKASDKPVIAFCGVHFMAET"
     gene            370..1260
                     /locus_tag="P5164_20765"
     CDS             370..1260
                     /locus_tag="P5164_20765"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRY46377.1"
                     /translation="MTFPRPARLLASALAALLLASALAETAEAATKRKRRTSRKAAPI
                     PSVPRPQDPFEVTFPTEDGVRLHATWKPSPAGAAAPAVLLVHAFSRERRDLAPLADEL
                     LARGFSTLAVDLRGHGESVWKGGARIGLSPSLQTSPSAFPRDVEAACAWLRSRASRVG
                     IVGFSLSGNLAALATASGWADAGVAVSAYADRLPALAGSRPTASRGLLVLASEADPGR
                     AESARALDAAGRDPKAVVVYPGAAHALDLLTLEPGAKKATIEWLEARLAPIPPFPVAT
                     AAPAAPEAAPAPTPPAEAVE"
     gene            1277..>1591
                     /locus_tag="P5164_20770"
     CDS             1277..>1591
                     /locus_tag="P5164_20770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46378.1"
                     /translation="MPHATTLVFDIETVGLPWLDLDPMLREWLTRGAEDGEAYRSKKD
                     WRSLSPYAAKIVVIAALNPETGHGKVWYEAAEPSSRPSRDGLFEEIGGDERALLSGFW
                     ETA"
BASE COUNT          192 a          622 c          569 g          208 t
ORIGIN      
        1 gtctcggcca tgaagtggac gccgcagaac gcgatgaccg gcttgtccga ggccttcccc
       61 ttccgggcca gctcgagcga gtcgccgacg aagtcggcga ggtcctggat ctccggctcc
      121 tggtagtagt gcgccaggat cagggcgtcg cgctcctcct tgaggcggag gatcgcgtcc
      181 ggcagctcgg ccggaagagg gatcgcaagc gtcgggagag gggtcgggga gctcgccacg
      241 aggccattct acggccccgc aggggcctcg agccctctgc taccatccgg agcttccggg
      301 ggttgcgacc atgagctccg tcgagacgac gaccttcgac acgagaggcg ggacggaggt
      361 cgcggaagga tgacgttccc gcgcccggcc cgcctcctcg cgtccgccct ggccgcgctc
      421 ctcctggcga gcgcgctcgc cgagacggcc gaggccgcga cgaagcggaa gcggcggacg
      481 agccggaagg ccgcgccgat cccgtccgtc ccgcggccgc aggacccgtt cgaggtgacg
      541 ttcccgacgg aggacggcgt ccgcctccac gcgacgtgga agccttcgcc ggcgggcgcg
      601 gcggcgccgg ccgtcctcct cgtccacgcc ttctcccgcg agcgccggga cctggcccct
      661 ctcgccgacg agcttctggc ccgcggcttc tcgacgctcg ccgtcgacct gcgcggccac
      721 ggcgagtcgg tctggaaggg gggcgcgcgg atcggcctct ccccctcgct ccagacctcc
      781 ccgtccgcgt tcccgcgcga cgtcgaggcc gcatgcgcgt ggctccggag ccgcgcgtcg
      841 cgcgtcggaa tcgtcggctt ctcgctctcg gggaacctgg ccgccctcgc gacggcctcg
      901 ggctgggccg acgccggggt cgccgtctcc gcgtacgccg accgtctccc ggcgctcgcg
      961 ggctcgcggc cgaccgcctc gcgcggcctc ctcgtcctcg cctccgaggc cgacccgggc
     1021 cgcgccgagt cggcgcgcgc cctcgacgcc gccggccggg acccgaaggc cgtcgtcgtc
     1081 tatccgggtg ccgcccacgc cctcgacctc ctcacgctcg agccgggagc gaagaaggcg
     1141 acgatcgagt ggctcgaggc gcgcctcgca ccgatccccc ccttccccgt cgcgacggcc
     1201 gcgcccgcgg cgccggaggc cgccccggcg ccgactccgc ccgccgaagc ggtggagtga
     1261 tcccggacgc ccggccatgc cgcacgccac cacgctggta ttcgacatcg agaccgtcgg
     1321 cctgccgtgg ctcgacctcg acccgatgct gcgcgagtgg ctcacgcggg gcgcggagga
     1381 cggcgaggcc taccggtcga agaaggactg gcgctcgctc tccccctacg ccgcgaagat
     1441 cgtcgtcatc gcggcgctca accccgagac ggggcacggc aaggtctggt acgaggcggc
     1501 cgagccctcc tcgcggccct cgcgcgacgg cctgttcgag gagatcggag gggacgagcg
     1561 cgcgctgctc tccggcttct gggagacggc c
//
LOCUS       DAOTEZ010000681         1570 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_74763_length_1570_cov_3.413861, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000681 DAOTEZ010000000
VERSION     DAOTEZ010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74763_length_1570_cov_3.413861"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="P5164_20775"
     CDS             complement(<1..1061)
                     /locus_tag="P5164_20775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HRY46379.1"
                     /translation="MPTGRNDPCPCGSGKKFKVCCLRLGRKAQAADEAFSTADLPARF
                     RENVASILSAGGDVATAEVRSAVVLAGELCLEDAAADDELLRPCHLAFAVMSELIRKN
                     GGGEEELVSVFLPRLPFDVVLDDEGRTAGDLLLERHGAMLPEPAREAVRALVEAEDAL
                     CRVEKVGGRVFIEDLRTGTRLPAAEAFRMDAPRMTTRLVRYRGRHVPLDPELIDEPDD
                     PWYADDLEDRFDHAEGFLKRFDLRLRSRGKGGGIGGELVELAFGEGRDAAERTPDVRN
                     AEGHRLVFTTLRWDVAWEPGVRTSLATVEGLELEETAAGLEGTFLKKQPPKARKMPGE
                     AIVVGSLKLGDGQLVVETNS"
     regulatory      1166..1260
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1341..>1570
                     /locus_tag="P5164_20780"
     CDS             1341..>1570
                     /locus_tag="P5164_20780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46380.1"
                     /translation="MTRAPKTKTRSLAGALALAPLLALAAFATAVEPEPPAAATGPAD
                     AEPAAERVPPLPRRTEEVVVQAVRADVDTPVT"
BASE COUNT          222 a          599 c          539 g          210 t
ORIGIN      
        1 gaattcgtct cgacgacgag ctgcccgtcc ccgagcttca gcgacccgac gacgatcgcc
       61 tcgccgggca tcttccgggc cttcggaggc tgcttcttca ggaacgtacc ttcgagcccg
      121 gcagcggtct cctccagctc gagcccttcc acggtcgcga gcgaggttcg gacaccgggc
      181 tcccaggcga cgtcccagcg aagcgtcgtg aagacgagac ggtgcccctc ggcgttccgg
      241 acgtcgggcg tgcgctccgc cgcgtcccgt ccctcgccga acgcgagctc cacgagctct
      301 cccccgatgc ccccgccctt cccgcggctc cggagcctca ggtcgaaccg cttcaggaag
      361 ccctcggcgt ggtcgaagcg gtcctcgagg tcgtccgcgt accaggggtc gtccggctcg
      421 tcgatgagct cgggatcgag gggtacgtgg cgccccctgt accggacgag gcgggtcgtc
      481 attcgcggtg cgtccattcg gaacgcctcc gccgccggga gcctcgttcc cgttcggagg
      541 tcctcgatga agacccggcc gccgaccttc tcgacgcggc agagggcgtc ctcggcctcg
      601 acgagggccc gcacggcctc gcgggccggc tccggaagca tggcgccgtg cctctcgagg
      661 aggaggtcgc cggccgtccg cccctcgtcg tcgaggacga cgtcgaacgg gagccgcggg
      721 aggaagaccg agacgagctc ctcctcgccg ccgccgttct tccggatcag ctcggacatc
      781 acggcgaaag cgaggtgaca ggggcggagc agctcgtcgt cggccgcggc gtcctcgagg
      841 cacagctcgc ccgcgaggac gacggcggac cggacctccg cggtggcgac gtcccctccc
      901 gcgctcagga tcgacgccac gttctcgcgg aaccgggccg gcaggtcggc cgtggagaac
      961 gcctcgtccg cggcttgggc cttccgtccg agacgcagac agcagacctt gaacttcttt
     1021 ccgcttccgc aggggcaggg gtcgttccgg cctgtcggca tgccgcggag tatgcggcga
     1081 tgacgcccac cgcgccgcgt gccgcacccg ccgcgcgtcc gcagcccgtt gacacgcggc
     1141 ccgctccgtc ccatattccc gcccgacgga tgcctagggg tggccgggag accggcctga
     1201 gagcagaccc tcgaacctga tccgggtcat accggcgaag ggaacggcaa acggaagcgg
     1261 gagcttccgc aaacctcgag agaccccgac ggatccgctt cgatcgcccg cgccagaggc
     1321 gcgggagagg aggcgcttcc atgacccgag ccccgaagac gaagacccga agcctcgcgg
     1381 gcgcgctcgc gctcgccccg ctcctcgcgc tggccgcgtt cgcgaccgcg gtcgagcccg
     1441 agccgccggc cgcggcgacc ggtccggccg acgccgaacc ggccgccgag agggtcccgc
     1501 cgctcccccg ccgcacggag gaggtcgtcg tccaggccgt ccgcgccgac gtcgacacgc
     1561 cggtcacgaa
//
LOCUS       DAOTEZ010000682         1566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_75067_length_1566_cov_7.751820, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000682 DAOTEZ010000000
VERSION     DAOTEZ010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75067_length_1566_cov_7.751820"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="P5164_20785"
     CDS             complement(<1..614)
                     /locus_tag="P5164_20785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011243918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HRY46381.1"
                     /translation="MIGDLGSVVGVAVGVVLFFGAAALVVVKRLFHICSPNEVLVFSG
                     GKSTVEGRPVGYRIVKGGSSLRTPLLERVDRMDITNMTVDVAVSNAYSKGGIPLTVQG
                     VANLKVAGHQPLLNNALERLLGRGRPQIIQVAKDTLEGNLRGVLSQLTPEEVNQDKLR
                     FAEKLLEEAEHDLSRLGLILDVLKIQNVADDVGYLNSLGRKQTAA"
     gene            complement(611..>1566)
                     /locus_tag="P5164_20790"
     CDS             complement(611..>1566)
                     /locus_tag="P5164_20790"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HRY46382.1"
                     /translation="VSSDPRVVGNAIERFLGRDPAEIARVAKESLEGHLRGVLANMTP
                     EEVNEDRLKFATGLVDEAEGDFRKLGLTLDTLKIQNISDEANYLANIGRARIAMVLRD
                     AEMAESLARSEASQGEAVSRRTGEVANQQAATAIAKKENELKTLKAQLEAKAKAAEET
                     AVQAALQARAEAEQKLQAIRKELEETRLTAEEVLPAQAAQQAAALAARGAAATIEENG
                     WALAEVLKLMTTAWVSAGPDAKDIFLIHNLEQILRTVVERVKEMEVGEAHLLDGGDGS
                     SLATYVASYPAAVRSVLAEVSRTTGVDVVALLSPARTEVAR"
BASE COUNT          195 a          531 c          579 g          261 t
ORIGIN      
        1 gcggcggtct gcttgcggcc gagggagttc aggtagccga cgtcgtcggc gacgttctgg
       61 atcttcagga cgtcgaggat caggccgagg cgggagaggt cgtgctcggc ctcctcgagg
      121 agcttctcgg cgaagcggag cttgtcctgg ttgacctcct cgggggtgag ctgcgagagg
      181 acgccgcgga ggttcccttc gagggtgtcc ttggcgacct gaatgatctg ggggcggccc
      241 ctgccgagga gccgctcgag ggcgttgttc aggagcggct ggtggcccgc gaccttgagg
      301 ttcgcgacgc cctgcaccgt gagcgggatg ccccccttgg agtaggcgtt cgacacggcg
      361 acgtcgacgg tcatgttcgt gatgtccatc cggtcgaccc gctcgaggag cggcgtccgg
      421 agggagctgc cccccttgac gatccggtag ccgacggggc gcccctcgac ggtcgacttc
      481 ccgccggaga agacgaggac ctcgttcggg gagcagatgt ggaagaggcg cttgacgacg
      541 acgagcgccg cggcgccgaa gaagaggacg acgccgaccg cgacgccgac gacggagccg
      601 aggtcgccga tcatcgcgcc acctccgtgc gggccgggga gaggagcgcg acgacgtcca
      661 cccccgtcgt ccggctcacc tcggcgagga cgctccgcac cgcggccggg tacgacgcga
      721 cgtacgtggc gagcgaagag ccgtcgccgc cgtcgaggag gtgggcctcg ccgacctcca
      781 tctccttcac ccgctcgacg acggtccgga ggatctgctc gaggttgtgg atgaggaaga
      841 tgtccttcgc gtcggggccg gcgctcaccc acgcggtcgt catcagcttg agcacctcgg
      901 cgagggccca gccgttctcc tcgatcgtcg ccgcggcgcc gcgcgccgcg agggccgcgg
      961 cctgctgggc cgcctgcgcc gggaggacct cctcggccgt caggcgcgtc tcctcgagct
     1021 ccttgcggat ggcctggagc ttctgctcgg cctcggcgcg ggcctggagc gcggcctgga
     1081 cggcggtctc ctcggcggcc ttcgccttcg cctcgagctg cgccttgagc gtcttcagct
     1141 cgttctcctt cttcgcgatc gccgtcgcgg cctgctggtt cgcgacctcg cccgtccggc
     1201 gggagacggc ttcgccctgg gaggcttcgg agcgggcgag cgactcggcc atctccgcgt
     1261 cgcgcaggac catcgcgatc cgggcgcggc cgatgttcgc gaggtagttc gcctcgtcgg
     1321 agatgttctg gatcttcagc gtgtcgagcg tcaggccgag cttgcggaag tccccctcgg
     1381 cctcgtcgac gaggccggtc gcgaacttga ggcggtcctc gttgacctcc tccggcgtca
     1441 tgttcgcgag gacgcctcgg aggtgcccct cgaggctctc cttggcgacg cgcgcgatct
     1501 cggccgggtc tcgcccgagg aagcgctcga tggcgttccc gacgacgcgc gggtcggagg
     1561 agacct
//
LOCUS       DAOTEZ010000683         1556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_75907_length_1556_cov_5.714857, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000683 DAOTEZ010000000
VERSION     DAOTEZ010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75907_length_1556_cov_5.714857"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="P5164_20795"
     CDS             <1..505
                     /locus_tag="P5164_20795"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HRY46383.1"
                     /translation="ETGHGKVWYEAAEPSSRPSRDGLFEEIGGDERALLAGFWETAAR
                     FDRLVSFNGRSFDGPFLSVRSAIHGVAPSRNLAGYRYSVESHVDLMEVLTFQGAAGTR
                     PSLHAACVAFGIPSPKSEEMHGYAVGDAYAEGRLPEILDYCRRDVEATAELLRRLEPT
                     LLPLFRR"
     gene            604..1014
                     /locus_tag="P5164_20800"
     CDS             604..1014
                     /locus_tag="P5164_20800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46384.1"
                     /translation="MNRKVIALVEDPFWRTKIDNAARSVGASTVFLSDAAGAAAAIEP
                     GAPVIVVVDLALKSSPFEALSALKAAKATASVPIVGYFDIARKDLKEKAVAAGFDEAL
                     ARSSFAERFADIVLKYLLPGGVRKEEDERELPEE"
     gene            complement(1102..>1556)
                     /locus_tag="P5164_20805"
     CDS             complement(1102..>1556)
                     /locus_tag="P5164_20805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HRY46385.1"
                     /translation="ISLATETDRTLFFDFLPLDLGTVRGFKTRFHLYTVPGQVFYDAS
                     RKLILKGVDGVVFVADSQEARMDANVESLQNLEKNLHEHGFDLATIPYVLQLNKRDLP
                     TAVAADEMYRQLNFKGEPTFEATAPTGVGVFDTLKAVAKQVLMELRKK"
BASE COUNT          225 a          535 c          551 g          245 t
ORIGIN      
        1 cgagacgggg cacggcaagg tctggtacga ggcggccgag ccctcctcgc ggccgtcccg
       61 cgacggcctc ttcgaggaga tcggagggga cgaacgggcg ctcctcgccg gcttctggga
      121 gacggccgcc cgctttgaca ggctcgtctc gttcaacgga cgctccttcg acggcccgtt
      181 cctctccgtg aggagcgcga tccacggcgt ggcgccgtcc cggaacctcg ccggctaccg
      241 ctactcggtc gagtcgcacg tcgacctcat ggaggtcctg acgttccagg gcgccgccgg
      301 gacccgcccc tcgctccacg ccgcctgcgt cgccttcggc atcccctccc cgaagtcgga
      361 ggagatgcac ggctacgccg tcggcgacgc ctatgccgag gggcggctgc ccgagatcct
      421 cgactattgc cggcgggacg tcgaggcgac ggccgagctc ctccggcgcc tcgagcccac
      481 gcttctcccg cttttccgac gctgagccgc ccgggaacgc gcccggccta tactcggcgc
      541 gtccctgcca tgcggaggag cagcgtccgc ggccctcgag acgcccgctc cgaggaggct
      601 tcgatgaatc gcaaggtcat cgccctggtc gaagacccgt tctggaggac gaagatcgac
      661 aacgcggcga ggtccgtcgg ggcctcgacg gtctttctct ccgacgcggc cggggccgcc
      721 gccgcgatcg agccgggggc tcccgtgatc gtcgtcgtcg acctggccct gaagagctcc
      781 ccgttcgagg cgctgtcggc gctgaaggcg gcgaaggcga cggcgtccgt gccgatcgtc
      841 ggctacttcg acatcgcgcg gaaggacctg aaggagaagg cggtcgcggc cggcttcgac
      901 gaggccctgg cccgctcgtc gttcgcggag cgattcgccg acatcgtcct gaagtacctc
      961 ctccccggcg gcgtccggaa ggaggaagac gagagggagc tccccgagga gtaggccgca
     1021 cccacgggtc cggcccggaa cgacgagagg cgggggaacc cgcctcttcg cgcctccgcg
     1081 ggtcctcgcg cgaggcccgg gctacttctt ccgaagctcc atcaagacct gtttggcgac
     1141 ggctttcagc gtgtcgaaga cgccgacgcc ggtcggggcg gtcgcctcga aggtgggctc
     1201 gcccttgaag ttcagctggc ggtacatctc gtcggccgcg acggccgtcg ggaggtcgcg
     1261 cttgttgagc tggaggacgt aggggatcgt cgccaggtcg aagccgtgct cgtggaggtt
     1321 cttctcgagg ttctggagcg actcgacgtt cgcgtccatc cgggcttcct gggaatcggc
     1381 gacgaacacg acgccgtcga cccccttgag gatcagcttc cggctcgcgt cgtagaagac
     1441 ctggccgggc accgtgtaga ggtggaagcg ggtcttgaag ccgcgcaccg tcccgaggtc
     1501 gagcgggagg aagtcgaaga agagcgtccg gtcggtctcg gtcgccaggg agatca
//
LOCUS       DAOTEZ010000684         1555 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_75997_length_1555_cov_4.134000, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000684 DAOTEZ010000000
VERSION     DAOTEZ010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75997_length_1555_cov_4.134000"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="P5164_20810"
     CDS             complement(<1..592)
                     /locus_tag="P5164_20810"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HRY46386.1"
                     /translation="MRIAVAMSGGVDSSVAALLLAREGAEVVGVSMHLWDHDREGGGS
                     GEGRCCTLDDLRTARRAAEAIGVPHYTLDLRERFLEAVVRPFVASYLKGETPVPCLAC
                     NTEVKFDALFRRARALGCEAVATGHYARLERDAETGELRLLKARDAAKDQTYFLYELT
                     PEQLAGARFPLGDLTKAEVRELAREAGLPNWDKPDSQ"
     gene            642..941
                     /locus_tag="P5164_20815"
     CDS             642..941
                     /locus_tag="P5164_20815"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011429926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HRY46387.1"
                     /translation="MPRPAPLSPGEIADRLASLPGWTFTEGALRRELVFADFPEAFAF
                     MTRVALAAEKMGHHPEWTNVWSRVSVALTTHDAGGVTELDFQLAGVMHRLMRNGD"
     gene            988..>1555
                     /locus_tag="P5164_20820"
     CDS             988..>1555
                     /locus_tag="P5164_20820"
                     /inference="COORDINATES: protein motif:HMM:NF024818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRY46388.1"
                     /translation="MEAPDSHEDLLELLSKSGKAVFAIDSSDRIVLWNTGAERLLGYP
                     AADVLGRSCHQAMSGRDVYGNRYCFGECPILRMRDRGETIQPFEIVLESRDAGPKRLR
                     CSILSIPGQRPELTTLVHILAETNGAHPDPGGFFQRFSGPVQHPSLAPLVPPRPTTSP
                     LTKREQEVLRLLSEGKKTDEMGEQLFISD"
BASE COUNT          233 a          595 c          499 g          228 t
ORIGIN      
        1 cctgcgagtc gggcttgtcc cagttcggca gccccgcctc gcgcgcgagc tcccggacct
       61 cggccttcgt caggtcgccg agcgggaacc gggccccggc gagctgctcg ggcgtcagct
      121 cgtagaggaa ataggtctgg tccttcgccg cgtcgcgggc cttgagcagc cgaagctctc
      181 ccgtctccgc gtcccgttcg aggcgcgcgt aatgccccgt ggccaccgcc tcgcatccca
      241 gcgcccgcgc tctccggaaa agcgcgtcga acttcacctc cgtgttgcag gcgaggcacg
      301 gcacgggcgt ctcgcccttc aggtacgacg cgacgaacgg gcggacgacc gcttcgagga
      361 accgctccct gagatcgagc gtgtagtgcg ggacgccgat cgcctcggcg gcccggcgcg
      421 ccgtgcgaag gtcgtcgagg gtgcagcagc gcccctcgcc ggacccgccg ccctcgcggt
      481 cgtggtccca caggtgcatc gagaccccga ccacctccgc tccctccctc gcgagaagga
      541 gcgccgcgac cgaggagtcg accccgcccg acatggcgac ggcgatccgc atacactcag
      601 gagtttacgt gccggggcca ccgcgccccg cggaggagcc gatgcctcgt cccgccccgc
      661 tctcccccgg cgagatcgcc gaccgcctcg cctcgcttcc cggctggacc ttcacggaag
      721 gcgcgctccg ccgcgagctc gtcttcgccg acttcccgga ggcgttcgcg ttcatgacgc
      781 gggtcgcgct cgcggcagag aagatgggcc atcaccccga gtggaccaac gtctggtcgc
      841 gcgtgtcggt cgcgctcacg acgcacgatg cgggcggggt caccgagctc gacttccagc
      901 tggccggggt gatgcatcgc ctgatgagaa atggggattg aagctggtgc attcgcatca
      961 gatccgaggg tagaatccgc ccccggaatg gaagcgcccg acagccacga ggacctcctc
     1021 gagctcctct ccaagagcgg caaggccgtc ttcgcgatcg actcctcgga ccgaatcgtc
     1081 ctctggaaca ccggcgcgga gaggctcctc ggctaccccg ccgccgacgt cctcgggcga
     1141 agctgtcacc aggcgatgtc gggccgggac gtctacggaa accgctactg cttcggggag
     1201 tgcccgatcc tccggatgcg cgaccgcggc gagacgatcc agcccttcga gatcgtcctc
     1261 gagtcccgcg acgccgggcc gaagcgcctc cgctgctcga tcctctcgat tcccggccag
     1321 cgccccgagc tgacgaccct cgtccacatc ctcgccgaga cgaacggcgc acatcccgac
     1381 cccggagggt tcttccagcg cttctcgggc cccgtccagc acccgagcct cgcgccgctc
     1441 gtcccgccgc gcccgacgac ctctccgctg accaagcgcg agcaggaggt cctccgcctt
     1501 ctctccgagg ggaagaagac cgacgagatg ggcgagcagc tcttcatcag cgacg
//
LOCUS       DAOTEZ010000685         1552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_76251_length_1552_cov_7.810955, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000685 DAOTEZ010000000
VERSION     DAOTEZ010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76251_length_1552_cov_7.810955"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..>1552
                     /gene="pruA"
                     /locus_tag="P5164_20825"
     CDS             181..>1552
                     /gene="pruA"
                     /locus_tag="P5164_20825"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR01236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HRY46389.1"
                     /translation="MSLGIYKVPVPKNETVKDYAPGSPERASLQKKLAEMASERIEIP
                     LVIGGKEIRTGKVAKAVMPHDHGHVLADVHLAGPEEVALAVAAADRAKDFWSRMPWEE
                     RVAIFLKAADLLAGPYRDVLNAATMLGQSKTCHQAEIDSAAETIDFFRFNAHYYAEIL
                     TEQPESGPGMWNRMEHRPLEGFVFAVSPFNFTAIASNLAAAPALCGNTVLWKPATTAL
                     YSAYWLTRLYRDAGMPEGVINMIPGSGRAIGDVVLNHADLAGIHFTGSTGVFNGMWRT
                     VGQNLEKFRSYPRLVGETGGKDFIFAHPSADPKALTTAILRGAFEYQGQKCSAASRAY
                     VPSNLWAKIQAELVSEVEAMKVGDPRDFTNFMGAVIDEAGFKDHTGAIDAAKASSDAK
                     IVAGGNYDSSRGWFVRPTIIQALDPSYHTMCHELFGPILTIHVYDEAKWEEMLDVVDA
                     TSPYALT"
BASE COUNT          273 a          556 c          520 g          203 t
ORIGIN      
        1 gcgccttcgg cggcgagaag gagacgggcg gcggccgcga gagcggctcg gacgcctgga
       61 aggcctacat gcgccgccag acgaacgccc tgaacttcag cggccgcctc gagctcgcgc
      121 agggcatcac gctcgagatc tgacccccgg atcgcgtcgg acacgagccc aggaggcccc
      181 atgtcgctcg gcatctacaa ggtcccggtc ccgaagaacg agacggtgaa ggactacgcg
      241 cccgggagcc ccgagcgcgc gagcctccag aagaagctcg ccgagatggc ctcggagcga
      301 atcgagatcc ccctcgtcat cggggggaag gagatccgga cggggaaggt cgcgaaggcg
      361 gtgatgccgc acgaccacgg ccacgtcctc gccgacgtcc acctcgccgg cccggaggag
      421 gtcgcgctcg ccgtcgctgc ggccgaccgc gcgaaggact tctggtcgcg gatgccgtgg
      481 gaggagcggg tcgccatctt cctgaaggcc gccgacctcc tcgccgggcc gtaccgcgac
      541 gtcctgaacg ccgcgacgat gctcggccag agcaagacct gccaccaggc cgagatcgac
      601 agcgccgccg agacgatcga cttcttccgc ttcaacgccc actactacgc cgagatcctg
      661 accgagcagc ccgagagcgg ccccggcatg tggaaccgga tggagcaccg gccgctcgag
      721 gggttcgtct tcgcggtctc gccgttcaac ttcacggcca tcgcgagcaa cctcgcggcg
      781 gcgccggccc tctgcggcaa cacggtcctc tggaagccgg cgacgacggc gctctacagc
      841 gcgtactggc tcacccgcct ctaccgcgac gcggggatgc ccgagggcgt catcaatatg
      901 atccccggaa gcgggcgcgc gatcggcgac gtcgtcctca accacgccga cctcgcgggc
      961 atccacttca ccgggtcgac gggcgtcttc aacgggatgt ggcggacggt ggggcagaac
     1021 ctcgagaagt tccgctccta cccgcgcctc gtcggcgaga ccggcggcaa ggacttcatc
     1081 ttcgcccacc cgagcgccga cccgaaggcg ctcaccacgg cgatcctccg cggcgccttc
     1141 gagtaccagg ggcagaagtg cagcgccgcc tcgcgcgcct acgtcccctc gaacctctgg
     1201 gcgaagatcc aggccgagct cgtctccgag gtcgaggcga tgaaggtggg cgacccgcgc
     1261 gacttcacga acttcatggg cgccgtcatc gacgaggcgg ggttcaagga ccacacgggg
     1321 gcgatcgacg cggcgaaggc gtcgagcgac gcgaagatcg tcgcgggcgg caactacgac
     1381 tcctctaggg gctggttcgt gcggccgacg atcatccagg cgctcgaccc ctcgtaccac
     1441 acgatgtgcc acgagctctt cgggccgatc ctcacgatcc acgtctacga cgaggccaag
     1501 tgggaggaga tgctcgacgt cgtcgacgcg acgagcccct acgcgctcac cg
//
LOCUS       DAOTEZ010000686         1546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_76772_length_1546_cov_5.992622, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000686 DAOTEZ010000000
VERSION     DAOTEZ010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76772_length_1546_cov_5.992622"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1546)
                     /gene="fusA"
                     /locus_tag="P5164_20830"
     CDS             complement(<1..>1546)
                     /gene="fusA"
                     /locus_tag="P5164_20830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRY46390.1"
                     /translation="RINIIDTPGHVDFTAEVERSLRVLDGAVAVFDAVSGVEPQSETV
                     WRQADRYGVPRIAFVNKMDRVGADFERCVEMMIAKLGAKPVPIILPWGSAETFRGVLD
                     VLEEKAYDFRDEALGADFDTVEVPAEHRADLAKWREKAIEAIAETDDELMHKYLEGVV
                     PSVDELKAALRRATIVGRLHPVVCGSAFKNKAVQQLLDSVVAYLPSPVDIPPIKGIDE
                     DGKEVSRETKDDAPFSGLVFKIMTDPFVGQLAFFRVYSGHLDAGSAVYNATKQTNERV
                     GRLLKMHANKREEIKQVWAGDIAAAVGLKNVVTGDTICEKGNVVVLEAMKFPEPVIEL
                     SIEPKTKADQEKMGLALAKLMQEDPTFRVSTDKETNQTIIAGMGELHLEIIVDRLIRE
                     FNVGANVGKPQVAYRESIRKEAQAEGRFVRQTGGKGQYGHAKIRLRPHPEGKEYEFVN
                     AIVGGVIPREFIKPIDQGIREAILTGVLAGYPTTKIQVELYDGSYHDVDSSEMAFQAA
                     ARGAFR"
BASE COUNT          214 a          514 c          539 g          279 t
ORIGIN      
        1 tcgcggaacg caccgcgggc cgccgcctgg aacgccatct ccgacgagtc gacgtcgtga
       61 tacgagccgt cgtagagctc gacctggatc ttcgtcgtcg ggtacccggc gaggacgccc
      121 gtgaggatcg cctcgcggat cccctggtcg atcggcttga tgaactcgcg cgggatgact
      181 ccgccgacga tcgcgttgac gaactcgtac tccttcccct cggggtgggg gcggaggcgg
      241 atcttggcgt ggccgtactg ccccttgccg cccgtctggc ggacgaagcg gccctcggcc
      301 tgggcctcct tccggatcga ctcgcggtag gcgacctgcg gcttgccgac gttcgcgccg
      361 acgttgaact cgcggatgag gcggtcgacg atgatctcga ggtggagctc gcccatcccg
      421 gcgatgatcg tctggttcgt ctccttgtcg gtcgagaccc ggaacgtcgg gtcttcctgc
      481 atgagcttcg cgagggcgag gcccatcttc tcctggtcgg ccttcgtctt cggctcgatg
      541 gagagctcga tgaccggctc cgggaacttc atcgcctcga ggacgacgac gttgcccttc
      601 tcgcagatcg tgtcgcccgt gacgacgttc ttcaggccca cggcggccgc gatgtcgccc
      661 gcccagacct gcttgatctc ctcgcgcttg ttcgcgtgca tcttgaggag ccggccgacg
      721 cgctcgttcg tctgcttcgt ggcgttgtag accgcggagc cggcgtcgag gtggcccgag
      781 tagacccgga agaaggcgag ctggccgacg aacgggtcgg tcatgatctt gaagacgagg
      841 cccgagaagg gcgcgtcgtc cttcgtctcg cgggagactt ccttcccgtc ctcgtcgatc
      901 cccttgatcg gcgggatgtc caccggggag ggaaggtagg cgacgacgga gtcgaggagc
      961 tgctggacgg ccttgttctt gaaggccgag ccgcagacga ccgggtggag cctgccgacg
     1021 atcgtggccc gccggagcgc ggccttcagc tcgtcgacgg aggggacgac gccctcgagg
     1081 tacttgtgca tcagctcgtc gtcggtctcg gcgatcgcct cgatcgcctt ctcgcgccac
     1141 ttcgcgaggt cggcgcggtg ctcggccggc acctcgacgg tgtcgaagtc cgccccgagc
     1201 gcctcgtcgc ggaagtcgta ggccttctcc tcgaggacgt cgaggacgcc gcggaacgtc
     1261 tccgcggagc cccaggggag gatgatcgga acgggcttcg ccccgagctt ggcgatcatc
     1321 atctcgacgc agcgctcgaa gtcggccccg acgcggtcca tcttgttgac gaacgcgatg
     1381 cgggggacgc cgtagcggtc cgcctggcgc cagacggtct cggactgcgg ctcgacgccc
     1441 gagacggcgt cgaagaccgc cacggcgccg tcgaggacgc gcagcgagcg ctccacctcg
     1501 gccgtgaagt ccacgtgccc cggcgtgtcg atgatgttga tccggg
//
LOCUS       DAOTEZ010000687         1542 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_77120_length_1542_cov_4.903161, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000687 DAOTEZ010000000
VERSION     DAOTEZ010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77120_length_1542_cov_4.903161"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="P5164_20835"
     CDS             complement(<1..332)
                     /locus_tag="P5164_20835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006170240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HRY46391.1"
                     /translation="MGKDAKLSRPYRVENGRKFRMKDFDPGDTGGLKLEKDHAAEKLE
                     KGVELLASLQDRLYAQDKRALLLVFQAMDAAGKDGTIKHVMSGVNPQGCQVFAFKTPS
                     AEELDHDFL"
     gene            complement(399..947)
                     /locus_tag="P5164_20840"
     CDS             complement(399..947)
                     /locus_tag="P5164_20840"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY46392.1"
                     /translation="MRPLRVVVTGSECTGKTTLARALAERFGAPWVPEFSRGYCEAKP
                     APLGPEDVEPIARGQMAGADAAEARATSLLVLDTDLLSTVVYARHYYGACPEWVEEAA
                     RARRADLYLLCAPDLPWSADGVRDRGEAREEMHRLFAEELARAGVRVISIAGPGPDRS
                     EAAAVAVAEAVARRSFAAGSAG"
     gene            complement(944..1540)
                     /gene="pnuC"
                     /locus_tag="P5164_20845"
     CDS             complement(944..1540)
                     /gene="pnuC"
                     /locus_tag="P5164_20845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HRY46393.1"
                     /translation="MNALEVAGFLTGILAVWLTAKESPWCWPTGIVNVALYAVVFAQA
                     KLYADMGLQGVFLAVCAYGWWEWLHGGEGGGRLAVSRASRPLLSGLVLAGAVFAAALG
                     WGLHRGTDAAFPFVDSTLAAFSLVAQYLQSVKKLETWWFWIAVDVGYVGMYLAKGLWL
                     TALLYAIFLALCVIGLRDWRRSLEAPPASDAAPAAEPA"
BASE COUNT          227 a          561 c          550 g          204 t
ORIGIN      
        1 aggaagtcgt ggtcgagctc ctcggccgac ggcgtcttga acgcgaagac ctgacacccc
       61 tgagggttca cgcccgacat gacgtgcttg atcgtcccgt ctttcccggc cgcgtccatc
      121 gcctggaaaa cgaggagaag ggccctcttg tcctgcgcgt agagcctgtc ctggagggag
      181 gcgagcagct cgaccccctt ctcgagcttc tcggccgcgt gatccttctc gagcttcagc
      241 ccgcccgtgt ctcccgggtc gaagtccttc atccggaact tcctcccgtt ctcgacgcgg
      301 taggggcggg agagcttcgc gtccttgccc atgtcggtct ccttttcccg atcggctcga
      361 tcggtctcgt tcccggggcg gaatgcgctc tggcgagtct atccggcaga gccggccgcg
      421 aaggagcggc gcgcgaccgc ctccgcgacc gcaacggcgg ccgcctcgga gcggtcgggt
      481 ccggggcccg cgatcgagat cacgcggacc ccggcccgcg ccagctcctc ggcgaagagg
      541 cggtgcatct cctcccgcgc ctcgccgcgg tcgcggaccc cgtcggcgct ccagggaagg
      601 tcgggcgcgc agaggaggta gaggtcggcc cgccgggcgc gggcggcctc ctcgacccac
      661 tcggggcacg cgccgtagta gtgccgggcg tagacgaccg tggagaggag gtcggtgtcg
      721 aggacgagga gactcgtcgc gcgcgcctcc gcggcgtcgg ccccggccat ctgcccgcgc
      781 gcgatcggct cgacgtcctc cggcccgagc ggcgccggct tcgcctcgca gtagccgcgg
      841 ctgaactcgg gcacccacgg cgcgccgaac cgctcggcga gcgcgcgcgc gagcgtcgtc
      901 ttccccgtgc actccgagcc ggtgacgacg acgcggagcg gcctcacgcc ggctccgcgg
      961 ccggggcggc gtccgaggcc ggaggcgcct cgagcgagcg ccgccagtcg cgcaggccga
     1021 tcacgcagag cgcgaggaag atcgcgtaga ggagcgccgt cagccagagc cctttcgcga
     1081 ggtacatccc gacgtacccg acgtcgacgg cgatccagaa ccaccaggtc tcgagcttct
     1141 tgactgactg gaggtactgg gcgacgaggc tgaaggcggc gagcgtcgag tcgacgaacg
     1201 ggaacgcggc gtccgtcccg cggtggagac cccagccgag cgccgccgcg aagacggcgc
     1261 ccgccaggac gagaccggaa aggagcgggc gcgaggcgcg cgaaaccgcg agcctcccgc
     1321 ccccctcgcc gccgtggagc cactcccacc agccgtaggc gcagacggcg aggaagaccc
     1381 cctgcagccc catgtcggcg tagagcttcg cctgcgcgaa gacgacggcg tagagcgcga
     1441 cgttgacgat cccggtcggc cagcaccacg ggctctcctt cgccgtgagc cagacggcga
     1501 ggatccccgt gaggaagccg gcgacctcga gcgcgttcag gt
//
LOCUS       DAOTEZ010000688         1541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_77207_length_1541_cov_6.621131, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000688 DAOTEZ010000000
VERSION     DAOTEZ010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77207_length_1541_cov_6.621131"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1541
                     /locus_tag="P5164_20850"
     CDS             <1..>1541
                     /locus_tag="P5164_20850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRY46394.1"
                     /translation="EADGKPQRVRKPHVNLGIAVDVAKKDGSRSLLVPNIKLAEGLDF
                     AEFVATFDNLVGKARRGAIEPEAFLGTSISLTNPGTLGTTSSSPRLMPGQGCIVATGA
                     MGYPPEYLAMPDEVVSGLGVSRIMAVTSTYDHRIVQGAESGAFLATLQELLVGGDDFY
                     ERIFRDLKVPHRPVRWEPDRNPPFLGRGNRLEAVEKQARILPLINFYRVRGHLLADLD
                     PLGVDAPRYHAELDPATFGYTIWDLDRKFITNGLAGRDQATLREILEVLRQTYCGKIG
                     AEFMNIQDPAQKAWLMERMESSRNRATLSVDEKKRVLAKLVEAESFEKFLHAKYVGHK
                     RFSLEGGEAAIPLLTRLLDRAAESGVEEAVIGMPHRGRLTILTTVVGKAVDRIFAEFE
                     DVLDPQSIQGSGDVKYHLGAVGVHEAPDGRRVKLGLAPNPSHLEAVDPVVEGMVRAKQ
                     ERAGDAAREKVLPILLHGDAAFAGQGLVAETLNMCQLEGYRTGGTIHVVVNNQIGFTT
                     NPKDAR"
BASE COUNT          249 a          547 c          536 g          209 t
ORIGIN      
        1 gaggcggacg ggaagccgca gcgcgtccgc aagccgcacg tgaacctcgg catcgccgtc
       61 gacgtcgcga agaaggacgg cagccgctcc ctcctcgttc cgaacatcaa gctcgccgag
      121 gggctcgact tcgccgagtt cgtcgcgacg ttcgacaacc tcgtggggaa ggcccgccgc
      181 ggcgcgatcg agccggaggc gttcctcggg acgtcgatct cgctgacgaa ccccgggacg
      241 ctcggcacga cgtcgtcctc gccgcgcctc atgcccggcc agggctgcat cgtcgcgacg
      301 ggggcgatgg gctatccgcc cgagtacctc gcgatgcccg acgaggtcgt ctccgggctc
      361 ggagtctccc ggatcatggc ggtgacgtcg acgtacgacc accggatcgt ccagggggcg
      421 gagtcgggcg cgttcctggc gacgctccag gagctcctcg tcggcggcga cgacttctac
      481 gagcggatct tccgcgacct gaaggtcccc caccgtccgg tccgctggga gccggaccgc
      541 aacccgccgt tcctcggccg ggggaaccgc ctcgaggcgg tcgagaagca ggcccgcatc
      601 cttccgctca tcaacttcta ccgggtccgc ggccatctcc tggccgacct cgacccgctc
      661 ggcgtcgacg ccccgaggta ccacgccgag ctcgacccgg cgacgttcgg ctacacgatc
      721 tgggacctcg accggaagtt catcacgaac ggcctcgcgg gacgcgacca ggcgacgctg
      781 cgcgagatcc tggaggtcct gcgccagacc tactgcggga agatcggcgc cgagttcatg
      841 aacatccagg accccgccca gaaggcgtgg ctcatggagc ggatggagtc gagccggaac
      901 cgggcgacgc tctccgtcga cgaaaagaag cgggtcctcg cgaagctggt ggaggcggag
      961 agcttcgaga agttcctcca cgccaagtac gtcggccaca agcgcttctc cctggagggc
     1021 ggcgaggcgg cgattccgct cctgacccgc ctcctcgacc gcgcggcgga gtcgggcgtc
     1081 gaggaggccg tcatcgggat gccgcatcgc ggtcggctca ccatcctgac gacggtcgtc
     1141 ggcaaggccg tcgaccggat cttcgcggag ttcgaggacg tcctcgaccc gcagtcgatc
     1201 cagggctcgg gcgacgtgaa gtaccacctc ggcgccgtcg gcgtccacga ggccccggac
     1261 gggaggcggg tgaagctcgg cctcgcgccg aacccgagcc acctcgaggc ggtcgacccg
     1321 gtcgtcgagg ggatggtgcg cgccaagcag gagcgcgcgg gcgacgccgc gcgcgagaag
     1381 gtcctcccga tcctcctcca cggcgacgcg gccttcgcgg ggcaggggct cgtcgccgag
     1441 acgctgaaca tgtgccagct cgaggggtac cggacgggcg gcacgatcca cgtcgtcgtg
     1501 aacaaccaga tcggcttcac gacgaacccg aaggacgccc g
//
LOCUS       DAOTEZ010000689         1541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_77210_length_1541_cov_5.849933, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000689 DAOTEZ010000000
VERSION     DAOTEZ010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77210_length_1541_cov_5.849933"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..>1541)
                     /locus_tag="P5164_20855"
     CDS             complement(30..>1541)
                     /locus_tag="P5164_20855"
                     /inference="COORDINATES: protein motif:HMM:NF036694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRY46395.1"
                     /translation="AIQKYAEANNLTKNLKETNLSGDDCREGLTAVVSLKIRDPKFSS
                     QTKEKLVSQEAKTWVQTVTYERLSAFFEENPKVARRIVDKIIEAARAREAARRARELV
                     RRKGALDGAGLPGKLADCQEADPALSELFIVEGDSAGGSAKQGRDRRTQAILPLRGKI
                     LNVEKARFDKMLAATEIRSLILALGAGIGEEFDVEKIRYHKVVLMTDADVDGSHIRTL
                     LLTFFFRQMRAALERGYIYIAQPPLYKVVDGKRESFLKDDKEYARFLLSRIGDDRALV
                     TEPTGASVSGAALVRLFTETQEWLSLVKRLALRGVPEELLRALVKAGLGPEAAREREV
                     LEALAARLPAGLSPEVHADEEHGGFALHVEREVNGVVRKTIVDAAFLGAFDIRRAGEL
                     AAALDGFLDGPYLLRRSDGERRVATLVEAADAILESAKKGLAVSRYKGLGEMNPETLW
                     ETTMNPESRRLLQVRVEDAVEADELFTILMGDAVEPRRAFIEANALAATNLDI"
BASE COUNT          207 a          535 c          534 g          265 t
ORIGIN      
        1 cggggggatc gagaaaaccc cgcttcatct cagatgtcca ggttcgtcgc cgcgagggcg
       61 ttcgcctcga tgaaggcgcg acggggctcg accgcgtcgc ccatcaggat cgtgaagagc
      121 tcgtcggctt cgaccgcgtc ctcgaccctc acctgcagga gacgccgcga ctccgggttc
      181 atcgtcgtct cccagagcgt ctccgggttc atctcgccga ggcccttgta gcggctgacc
      241 gcgaggccct tcttcgcgct ctccaggatc gcgtcggccg cctcgacgag agtggcgacc
      301 cgcctctccc cgtcgctgcg gcggaggagg taggggccgt cgaggaagcc gtcgagcgcg
      361 gccgcgagct ccccggcccg gcggatgtcg aaggcgccga ggaaggcggc gtcgacgatc
      421 gtcttgcgga cgacgccgtt cacctcgcgc tcgacgtgga gggcgaagcc gccgtgctcc
      481 tcgtcggcgt ggacctccgg cgacagcccg gccggaagcc gcgccgccag cgcttccagg
      541 acctcccttt cgcgcgccgc ctcgggtccc aggccggcct tcacgagcgc gcgcaggagc
      601 tcctccggca cgccccggag ggcgagccgt ttgacgagcg agagccactc ctgcgtctcg
      661 gtgaagagcc ggacgagggc ggcgccggag acggaggctc cggtcggctc ggtcacgagg
      721 gcccggtcgt cgccgatccg cgagaggagg aaccgcgcgt actccttgtc gtccttcagg
      781 aagctctcgc gctttccgtc gaccaccttg tagagcggcg gctgcgcgat gtagatgtag
      841 ccgcgctcga gcgccgccct catctgccgg aagaagaagg tcaggaggag cgtccggatg
      901 tgcgagccgt cgacgtcggc gtccgtcatc aggacgacct tgtggtagcg gatcttctcg
      961 acgtcgaact cctcgccgat tcctgcgccg agcgcgagga tcagggagcg gatctccgtc
     1021 gcggcgagca tcttgtcgaa gcgcgccttc tcgacgttga ggatcttccc gcggagcggg
     1081 aggatcgcct gagtcctccg gtcgcgcccc tgtttggccg agcctcccgc cgagtcgccc
     1141 tcgacgatga agagctcgga gagcgccggg tcggcctcct ggcagtcggc gagcttgccg
     1201 ggaaggcccg cgccgtcgag ggcgcctttc cgcctcacga gctcgcgggc ccttcgcgcg
     1261 gcctcccgcg cccgggccgc ctcgatgatc ttgtcgacga tccgccgcgc gaccttcggg
     1321 ttctcctcga agaaggccga gagcctctcg taggtcaccg tctggaccca cgtcttggct
     1381 tcctgggaga cgagcttctc cttcgtctgc gaggagaact tcgggtcgcg gatcttgagc
     1441 gagacgacgg ccgtgaggcc ttcccggcag tcgtcgcccg agaggttcgt ctccttgagg
     1501 ttcttcgtga ggttgttggc ctcggcgtac ttctggatcg c
//
LOCUS       DAOTEZ010000690         1540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_77296_length_1540_cov_5.203367, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000690 DAOTEZ010000000
VERSION     DAOTEZ010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77296_length_1540_cov_5.203367"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="P5164_20860"
     CDS             complement(<1..1232)
                     /locus_tag="P5164_20860"
                     /inference="COORDINATES: protein motif:HMM:NF024810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HRY46396.1"
                     /translation="MTQFAVPAHTVRFRVFVSALSSVFLLLATEARSTVQATGSMAAP
                     RSEHTATFLGTGKVLVAGGTNATGRLATAELFDPATGAFVAAAPMPEARHGHTATLLP
                     NGKVLVVGGNGDAGALATCRLFDPRTGAWTSTGSMATARREHTATLLASGKVLVAGGQ
                     NGTAPGYTALSSAELYDPATGTWAATAALPSARAGHAAALLENGKVLVAGGQTKWFPL
                     PALFTSTAYLFDPATETWSATGSMAATRRSFTMTRIPGGLVVAAGGRNASYLSSAERY
                     NVSTGAWGSTGGLGGARGGHAAALLPDARILLVGGMDGTGPIGDMAEYDPAANTWAEV
                     ETGGARYDHTVTLLPGGRVLAVGGERTGWLSDAELVDRANPAWTSAGTTWGRKDGTAT
                     ALPNGKVLVLGAASVGDVT"
     gene            complement(1437..>1540)
                     /locus_tag="P5164_20865"
     CDS             complement(1437..>1540)
                     /locus_tag="P5164_20865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012139979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HRY46397.1"
                     /translation="LVTGKKLHDKRETIKRREADRESRAAIKAGRTR"
BASE COUNT          199 a          609 c          498 g          234 t
ORIGIN      
        1 gtgacgtcgc cgaccgaggc cgctccgagg acgaggacct tgccgttcgg cagcgccgtc
       61 gccgttccgt ccttccgccc ccacgtcgtc cctgccgacg tccaggccgg gttggcccgg
      121 tcgacgagct cggcgtccga gagccagccc gtccgctcgc cgccgacggc gagcacgcgt
      181 cctcccggca ggagcgtgac ggtgtggtcg tatctcgctc cgcccgtctc cacctccgcc
      241 caggtgttcg cggccgggtc gtactccgcc atgtcgccta tgggtccggt cccgtccatt
      301 cctcccacga gcaggattcg ggcgtcgggc agcagcgcgg ccgcgtggcc gccccgcgcc
      361 ccaccgaggc ctccggtgct cccccacgcc ccggtggaga cgttgtagcg ctccgcgctc
      421 gagaggtagc tcgcgttccg gcctcccgcc gccacgacga ggcccccggg gatccgcgtc
      481 atggtgaacg accttctcgt cgcggccatc gaccctgtcg cgctccacgt ctccgttgcc
      541 gggtcgaaga ggtacgccgt cgacgtgaag agagccggca gcgggaacca cttcgtctgt
      601 cctcccgcca cgagaacctt cccgttctcg agcagcgccg ccgcgtgccc ggctcgcgcg
      661 ctcggcaacg ccgccgttgc cgcccatgtc cccgtcgccg ggtcgtacag ctccgcgctc
      721 gagagcgcgg tgtagccggg cgccgtgccg ttctgcccgc ctgccacgag gaccttgccg
      781 ctggccagca gcgtcgccgt gtgctcccgc cgcgccgtcg ccatcgaccc cgtcgaggtc
      841 cacgcccccg tccgcgggtc gaagagccga cacgtcgcga gagctcccgc gtcgccgttt
      901 ccgccgacga cgagcacctt gccgttcggc aggagcgtcg ccgtgtgccc gtgccgtgcc
      961 tccggcatcg gggcggcggc gacgaaagcg cccgtcgccg ggtcgaacag ctccgccgtc
     1021 gcgaggcgcc ccgtggcgtt cgttcccccc gcgacgagga ccttccccgt cccgaggaac
     1081 gtcgccgtgt gctccgagcg cggcgcagcc atcgaccccg tcgcctgcac ggtcgatcgt
     1141 gcttccgtcg cgagaagcag gaagacggac gacagggccg acacgaagac tcgaaagcgc
     1201 acggtatgag ccggaactgc gaactgcgtc atgagtcacc tccgccgcga ctgttccgaa
     1261 gacgctcgcg tggccacccc agcggcagcg atcagattac gacggaaccg tcccggcgcg
     1321 acgcgacgag acggaatttc atttcgtcac gaagcgggac gacgattcag agccctccaa
     1381 cgagcgccgc gacgacctcc cgatgcgatc gcacggatcc cgacctcagc gcccccctac
     1441 cgcgtccgcc ccgccttgat tgccgcgcgg ctctcgcggt cggcttcgcg ccgcttgatc
     1501 gtctcgcgct tgtcgtggag cttcttgccg gtgacgagcg
//
LOCUS       DAOTEZ010000691         1531 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_78103_length_1531_cov_6.466125, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000691 DAOTEZ010000000
VERSION     DAOTEZ010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78103_length_1531_cov_6.466125"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1444
                     /locus_tag="P5164_20870"
     CDS             <1..1444
                     /locus_tag="P5164_20870"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRY46398.1"
                     /translation="HDKGLVHRDLKPENVLVDGQGNAKIADFGLVRWIYPHSAAPTPR
                     DEDPAGAAALTQTGFVVGTLGYMSPEQVRGEPVDARSDLFAFGVLVFELLTGSSPFAR
                     SSPDAALDAVLNHEPSSLTVLVAGVPERLARVVAWCLEKQPGQRPASARALLPHLESI
                     QRELATAPLEPTAALPPLPPPGAARRSPSPLAWLAGALAVLLAFAGGFAAGRSRPAPA
                     PEATLAGAWTAHAVPLPASVPAEMALLPGGRKAILGSGELLDTTTGALRPRALGGALT
                     GLVVGRTGSAVAATSAAGVVEAPLPAGTPLRTLVPGAAEPAFSRDESLLAYVRREGGT
                     SELWVAKRDASEARRVARAEATLWAWPAFSADGESLLFYDVERGGPNAGTGYLFSVRI
                     ADGTPVPFGPDVRLDPRGRPAVARDGSIAVLEASSRRALLLEREGRSSVALPFGAGLV
                     VLTASPEGGTLATRSESGPLVLWRRVPGAT"
     gene            complement(1437..>1531)
                     /locus_tag="P5164_20875"
     CDS             complement(1437..>1531)
                     /locus_tag="P5164_20875"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002803929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRY46399.1"
                     /translation="PQLGYGDRGAGNVIPPGATLLFEVELLGVK"
BASE COUNT          154 a          623 c          554 g          200 t
ORIGIN      
        1 gcacgacaag gggctcgtcc accgcgacct gaagcccgag aacgtcctcg tcgacgggca
       61 ggggaacgcg aagatcgccg acttcggcct ggtccgctgg atctacccgc actcggccgc
      121 acccaccccg cgcgacgagg accccgccgg cgcggcggcg ctgactcaga ccggtttcgt
      181 cgtcgggacg ctcggctaca tgagccccga gcaggtgcgc ggcgagccgg tcgacgcgcg
      241 gagcgacctc ttcgcgttcg gcgtcctcgt cttcgagctc ctgacgggct cgtcgccgtt
      301 cgcccgttcg agccccgacg cggcgctcga cgccgtcctg aaccacgagc cttcgtcgct
      361 cacggtcctc gtggcgggag tcccggaacg gctcgcacgg gtcgtcgcct ggtgcctcga
      421 gaagcagccc gggcagcgcc ccgcctcggc ccgtgccctc ctgccccacc tcgagtcgat
      481 ccagcgcgag ctcgcgaccg cacctctcga gccgacggct gcgctccctc ccctccctcc
      541 gccgggggcg gcccggcgca gcccgtcccc gctggcctgg ctcgccggcg cgctcgccgt
      601 tctcctggcg ttcgccggcg gcttcgccgc cggccgttcc cggcccgccc ccgccccgga
      661 ggcgacgctc gccggagcct ggaccgcaca cgccgtcccg ctcccggcgt ccgtccccgc
      721 cgaaatggcc ctcctgccgg gcggccgcaa agcgatcctc gggagcggcg agctgctcga
      781 cacgacgacg ggggcgctcc gcccgcgcgc gctcggcggc gcgctgacag ggctggtggt
      841 cggccggacc ggctcggcgg tggcggcgac gtccgccgcc ggcgtcgtgg aggcgcccct
      901 gccggccggc acgccgcttc ggacgctcgt tccgggcgcc gcggagccgg ccttctcccg
      961 cgacgaatcg ctcctcgcgt acgtgcggcg cgaggggggg acgagcgagc tctgggtcgc
     1021 gaagcgcgac gcatccgagg cgcgccgcgt ggcgcgtgcc gaggcgacgc tctgggcctg
     1081 gcccgccttc tcggccgacg gggagagcct cctgttctac gacgtcgagc ggggcggccc
     1141 gaacgccggg accggctacc tcttctcggt gaggatcgcc gacgggactc ccgtcccgtt
     1201 cggccccgac gtgcgcctcg acccgcgcgg ccgcccggcc gtcgcccgcg acggcagcat
     1261 cgccgtcctc gaggcctcct cccgccgggc gctcctcctc gagcgcgagg ggcgctcgtc
     1321 ggtcgcgctg ccgttcgggg cgggcctcgt cgtcctcacg gcgtcgccgg agggagggac
     1381 gctcgcgacc cgctcggaga gcgggccgct cgtcctctgg aggcgggtcc ccggggctac
     1441 ttgacgccga gcagctcgac ctcgaacagg agcgtcgcgc cgggcgggat gacgttcccc
     1501 gcgccgcggt cgccgtagcc gagctgcggc g
//
LOCUS       DAOTEZ010000692         1529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_78300_length_1529_cov_6.184532, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000692 DAOTEZ010000000
VERSION     DAOTEZ010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78300_length_1529_cov_6.184532"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1362)
                     /locus_tag="P5164_20880"
     CDS             complement(145..1362)
                     /locus_tag="P5164_20880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRY46400.1"
                     /translation="MPGKAAPEGASIRVRGRLDAKGSAALENDLNEAVRAGVRRLRLD
                     LMEVPFLSSAGIRVLLKGQQAVARLGGEMTIVAASPTVREVLRLGGLEELLGSLGEAE
                     APAPPSVELVAGLSGHVHALAGGPLEGRLVGDPGRLPSGAFDAASMTSLLFPPGTIGL
                     GVGAFGEGFEDCRGRFGEFLAAGGAAACLPTDGSEPDDVVARGALVPTVQALYGVRCT
                     GAFSRLFRFEADAAAGPVPFSRLTAAALELAASEAAAFVVVAESAGLLGASLRRSPVD
                     AGGLFAFPSIRESLSFTAERVHQRSTALVVGVAAGVDGTPLAPFLRPAGSVPLPAVHA
                     HAAAFTFRAIPAGAPAVETVVRGLFDEERLLTLLHLLPDTREISGGGESLFLSGALWA
                     SPLRAGSPAGGAA"
BASE COUNT          228 a          541 c          623 g          137 t
ORIGIN      
        1 ggaggaccgc cgccgtcgcc gctccgaggg tgatcgagcc gtcggtcgtg atgtcggcga
       61 cgtcgaagac ccggaaggag acgtagacgc cgagcgcgag gatcgagagg aggaagccga
      121 tcgtgagcga gccgacgagg aggttcacgc ggcgcctccc gcggggctgc ccgcgcgcag
      181 cggcgaggcc cagagcgcgc cggagaggaa gaggctctcg cctccgccgc tgatctcccg
      241 cgtgtcgggg aggaggtgga ggagggtgag gagccgctcc tcgtcgaaga ggccgcggac
      301 gaccgtctcg acggcgggcg ccccggcggg gatcgcccgg aacgtgaagg ccgcggcgtg
      361 cgcgtggacg gccggcaggg ggacggagcc cgccggccgg aggaacggcg cgagcggcgt
      421 cccgtcgacg ccggcggcga cgccgacgac gagggccgtc gaccgctggt ggacgcgctc
      481 cgccgtgaac gacagcgact cgcggatcga gggaaaggcg aagaggccgc ccgcgtcgac
      541 gggcgagcgg cgcagcgagg cgccgaggag gccggccgac tccgccacga cgacgaacgc
      601 cgccgcctcg gaggcggcga gctcgagggc cgccgccgtg agacgcgaga aggggaccgg
      661 tccggccgcg gcgtcggcct cgaagcggaa gagccgggag aaggcgccgg tgcagcggac
      721 gccatagagg gcctggacgg tcgggacgag ggccccgcgc gcgacgacgt cgtcgggctc
      781 gctcccgtcc gtcggcagac aggccgcggc cccgcccgcg gcgaggaact cgccgaaccg
      841 cccgcggcag tcctcgaacc cctcgccgaa agccccgacg ccgaggccga tcgtgccggg
      901 agggaagagg aggctcgtca tcgacgcggc gtcgaaggca cccgagggga gccggcccgg
      961 atcgccgacg agccggcctt cgagagggcc gcccgcgagc gcgtggacgt ggccggagag
     1021 cccggcgacg agctcgaccg agggcggcgc gggcgcctcg gcttccccga ggcttccgag
     1081 gagctcctcc agcccgccga gccggaggac ctctcggacg gtaggcgagg ccgcgacgat
     1141 cgtcatctcg cccccgagac gcgcgacggc ctgctggccc ttgaggagga cccggattcc
     1201 cgcggagctg aggaagggga cctccatcag gtcgagccgg agccggcgga cgcccgcgcg
     1261 gaccgcctcg ttcaggtcgt tctcgagggc cgcggagccc ttcgcgtcga ggcggccccg
     1321 gacgcggatc gaagccccct cgggcgcggc tttccccggc acggcgctca tcgtcccgcc
     1381 acccggtcgg cccgcgcgag gagcgcgggc gggatcgtca ttccgagcgc cgccgccgcc
     1441 tcggggttga cgacgagcga ggtccggctc acgagctcga atgggatcga cgcggggctc
     1501 tccccgcgca gcagccgggc cgcaagccg
//
LOCUS       DAOTEZ010000693         1526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_78568_length_1526_cov_6.360299, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000693 DAOTEZ010000000
VERSION     DAOTEZ010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78568_length_1526_cov_6.360299"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..>1526
                     /locus_tag="P5164_20885"
     CDS             208..>1526
                     /locus_tag="P5164_20885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRY46401.1"
                     /translation="MSTAPSPATSAPSAVTVNDFSLQVATANGSGSQTANNVLLRSLF
                     RMGVPVSGKNLFPSNIQGLPTWFTIRASRHGYIGRKRELDVLVLMNPETSAADTLAAA
                     PGTSVVYERKLAVEKLRDDLRLFAVPFAELAAKLPLPPEQAKLRRLLVNMIYVGVLSD
                     LMGIDPVEIEASLRKQLGGKAKAIDLNLAALRVGLDYAAATFAEKLPHRVERMDATAG
                     KLIIDGNSASALGCLFGGATVCTWYPITPSSSLCETFIELCDRHRTDPATGRTAAAIV
                     QAEDEIASIGMVLGAGWAGARAFTATSGPGISLMSELIGYGYYAEIPAVIFDVQRVGP
                     STGLPTRTMQGDVLLCHTNSHGDGRHPVLFPSSPEECFTMAQEAFDLAERLQTPVFVL
                     TDLDLGMNNWMADPFPYPEKPFDRGKVLDADGVERLKDTWGRYRDLDG"
BASE COUNT          218 a          580 c          494 g          234 t
ORIGIN      
        1 gcgggcttgc gggcggcggc gcgcttcttc gccggcgccg gccgacccgg cttcttcggt
       61 ttcttctgcg ccatcttcgc gtgacctccg aaatcgaagg atacctccgg cccggcgggg
      121 ccggtcgccg gttcggtgta cattctcggg ttcacccgga cccgcgcgca ctcgaggcca
      181 gcggccgtcg gggggaggtg tttccgaatg tcgaccgctc catcgcccgc cacgagcgcc
      241 ccgtccgccg tcaccgtcaa cgacttctct ctccaggtcg ccacggcgaa cggctccggc
      301 tcgcagacgg cgaacaacgt cctgctccgc tccctgttcc ggatgggggt ccccgtctcg
      361 gggaagaacc tgttcccctc gaacatccag gggctcccga cctggttcac gatccgggcg
      421 agccgccacg gctacatcgg gcgaaagcgg gagctcgacg tcctcgtcct gatgaacccg
      481 gagacgtccg ccgccgacac cctggccgcc gcgcccggca cctccgtcgt ctacgagagg
      541 aagctcgccg tcgagaagct ccgggacgac ctccgcctct tcgcggtgcc gttcgccgag
      601 ctcgccgcga agctcccgct cccgccggag caggcgaagc tccggcgcct cctcgtgaac
      661 atgatctacg tcggcgtcct ctcggacctg atggggatcg acccggtcga gatcgaggcc
      721 tcccttcgca agcagctcgg cgggaaggcg aaggcgatcg acctgaacct cgcggccctc
      781 cgcgtgggcc tcgactacgc ggcggcgacg ttcgccgaga agctccccca ccgggtcgag
      841 cggatggacg caaccgcggg caagctgatc atcgacggca actccgcgtc ggccctcggc
      901 tgcctcttcg gcggggcgac ggtctgcacc tggtacccga tcaccccctc gtcgtctctc
      961 tgcgagacgt tcatcgagct ctgcgaccgc caccggaccg acccggcgac gggacgcacc
     1021 gcggccgcga tcgtccaggc cgaggacgag atcgcctcca tcggcatggt cctcggcgcc
     1081 ggctgggcgg gggcgcgcgc cttcacggcc acgtccggcc ccggtatctc gctcatgtcg
     1141 gagctgatcg gctacggcta ctacgccgag atcccggcgg tgatcttcga cgtccagcgc
     1201 gtcggcccct cgacgggcct cccgacgcgg acgatgcagg gagacgtcct cctctgccac
     1261 acgaactccc acggcgacgg gcggcacccg gtcctcttcc cctcgagccc cgaggagtgc
     1321 ttcacgatgg cccaggaggc gttcgacctc gccgagcggc tccagacgcc cgtcttcgtc
     1381 ctgaccgacc tcgacctcgg gatgaacaac tggatggccg accccttccc gtaccccgag
     1441 aagccgttcg accgcggaaa ggtcctggac gccgacggcg tggagcggct gaaggacacg
     1501 tggggccggt acagggacct cgacgg
//
LOCUS       DAOTEZ010000694         1511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_79872_length_1511_cov_6.263049, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000694 DAOTEZ010000000
VERSION     DAOTEZ010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79872_length_1511_cov_6.263049"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    277..303
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<304..513)
                     /locus_tag="P5164_20890"
     CDS             complement(<304..513)
                     /locus_tag="P5164_20890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46402.1"
                     /translation="MSVLGWLPRKLGRIVADQVHDRLAPVLRDVDGRLSALAQRSGPT
                     PIAGMGGSTISFDYPTSAANRPRWTA"
     gene            complement(913..>1511)
                     /locus_tag="P5164_20895"
     CDS             complement(913..>1511)
                     /locus_tag="P5164_20895"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548053.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRY46403.1"
                     /translation="PVGHAHFVTSTTHKTLRGPRGGLVLCTPEWAKEIDKAVFPGLQG
                     GPLEHVIAAKAVSFLEALSPEFREYQGRVLTNAKAFAEAVAGQGFRIVSGGTDNHLFL
                     VDLGPKQLTGKVAEKALDHAGITVNKNAIPFDPNPPLVTSGLRLGAPAVTTRGMGVAE
                     MKTIAGWIGEVLAAPEDEAVRVAVKAKVMELTAAFPIY"
BASE COUNT          212 a          535 c          525 g          212 t
ORIGIN      
        1 aacttcgtgg aattgcccga gccgatctcg agcacctgcc gcgtacccgg tccgaggaac
       61 gccgcgaacg ccgtgccgtc gagcccggcc atcatcgcgt tccgccagcg cgggcgcgga
      121 tcggcgtcgt ccgccgtctc ggggatcccg ctcccgccga gggctcgctc gaggtctccg
      181 gcgcgggcgg cgatcctacg gacggtctct gtccagaccg gttcgtcccg ctcgatcagc
      241 cgtcgaaggc cgaggtgccc cccccccccc ccccccnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnggccgtc caccgcggcc ggttggccgc cgaggtcgga tagtcgaaag aaatcgtcga
      361 tccgcccatc cctgcaatcg gcgtcgggcc ggaccgctgg gcgagagccg agagtcgccc
      421 atccacgtcg cgcaggaccg gcgcgagccg atcatggacc tggtcggcga cgatccgacc
      481 gagcttcctg gggagccatc cgagcacgct catgaaccgc ccatcctagc aagccccgcc
      541 ggagcccacg cgcacggcag atcgaaagtt cgtcggattc cgccggaccg gaaccgcgca
      601 cggagacggt gccaggcgtg aaatcgtgta ttttctctga aatacacgat ttcacgcctg
      661 gcaccacttg gtctggcacc acttggtccg ggcggtaccg ggttccactc cggcgctcgg
      721 gaagcgagcc gcctctccgc cgcggagggg gccgaggggg aacgggggag gcgggtcacg
      781 cctcccccga acctcacgcg ccgggacccg ggcggtcccg ggtccccgcg agctcgccgc
      841 ccgcgcagcg ggccgccgct ccggcgcggg ggggttcggg ggacccggac ggaaccgggt
      901 cccccgagaa cgctagtaga tcgggaacgc cgcggtcagc tccatgacct tcgccttgac
      961 ggcgaccctg accgcctcgt cctccggcgc cgcgaggacc tcgccgatcc agccggcgat
     1021 cgtcttcatc tcggcgacgc ccatgccgcg cgtcgtgacg gcgggggcgc cgagacgaag
     1081 gcccgaggtg acgagaggcg ggttcggatc gaacgggatc gcgttcttgt tgaccgtgat
     1141 cccggcgtgg tcgagcgcct tttcggcgac cttcccggtg agctgcttcg ggccgaggtc
     1201 gacgaggaag aggtggttgt cggtgccgcc ggagacgatc cggaagccct gcccggcgac
     1261 cgcctcggcg aacgccttcg cgttcgtcag gacacgcccc tggtattccc ggaactcggg
     1321 cgagagcgct tcgaggaacg agaccgcctt cgccgcgatg acgtgctcga gcggcccgcc
     1381 ctggaggccc gggaagaccg ccttgtcgat ctccttggcc cattcgggcg tgcagaggac
     1441 gaggccgccg cgggggccgc ggagcgtctt gtgcgtcgtc gacgtgacga agtgggcgtg
     1501 gccgacgggc g
//
LOCUS       DAOTEZ010000695         1508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_80167_length_1508_cov_5.941500, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000695 DAOTEZ010000000
VERSION     DAOTEZ010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80167_length_1508_cov_5.941500"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="P5164_20900"
     CDS             complement(<1..1096)
                     /locus_tag="P5164_20900"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY46404.1"
                     /translation="MSVPSILAEILAARRKRLAAGELAPRGTASLPSDGARFLAALRA
                     GGPRVIAEVKHRSPSAGAMLPGGSSGLSGAGDDLLPAIRSVAKAYRRAGAAAISVVTE
                     PDFFGGDLAWLPEVKRVSGLPVLMKDFVVDEVQLDLALSLGADAVLLIVAALTDDALV
                     RLHLSARARGLAVIVEAHDEEEVRRALAAGAEVVGLNARDLRTFAVDLGRVAAMGSLL
                     PPSVVRVAESGIHRPEDVSRLTASGFDAFLVGESLLRSGDPARALRALRGEGTTEVKV
                     CGVTREEDLAAAEELGADWVGLNFSTRSLRRVDLAHGRALREASRFAKGVVAVVAGNR
                     VPEAEAIVDAVRPDALQWHDDLAAREGLRVP"
     gene            complement(1093..>1508)
                     /locus_tag="P5164_20905"
     CDS             complement(1093..>1508)
                     /locus_tag="P5164_20905"
                     /inference="COORDINATES: protein motif:HMM:NF012800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRY46405.1"
                     /translation="VLAHALAALGAAHALVVSNSGAGDELLPHGLTIVAEVAEGEVRL
                     EEWTAATFGLSERDPREMAGGNAETNAGILRSILDGEEGPRRDAVLMNAAAALLVSGV
                     ALDLGDGMARAAASIDSGAARRALERLAAISQEAA"
BASE COUNT          199 a          581 c          572 g          156 t
ORIGIN      
        1 cggggacgcg cagcccctcc cgcgccgcga ggtcgtcgtg ccactggagc gcgtcgggcc
       61 gaaccgcgtc gacgatcgct tccgcctcgg gcaccctgtt ccccgcgacc acggcgacga
      121 cccccttcgc gaagcggctc gcctcgcgaa gcgcccggcc gtgcgcgagg tcgacccgcc
      181 gcagcgagcg ggtcgagaag ttgagaccga cccagtcggc ccccagctcc tcggccgcgg
      241 cgaggtcctc ctcgcgcgtc acgccgcaga ccttgacctc cgtcgttccc tcgccgcgca
      301 gcgcgcggag cgcgcgggcc gggtcgccgg agcgcaggag cgactcgccg acgagaaagg
      361 cgtcgaagcc cgaggccgtc aggcgcgaga cgtcctcggg gcgatggatg ccgctctcgg
      421 cgacgcggac gaccgacggc ggcagcaggc tccccatcgc ggcgacgcgc ccgaggtcga
      481 cggcgaacgt ccggaggtcg cgcgcgttca ggccgacgac ctccgccccc gcggcgagcg
      541 cccggcggac ctcctcctcg tcgtgcgcct cgacgatcac cgcgaggccg cgcgcacggg
      601 cagagaggtg gagccgaacg agcgcgtcgt ccgtcagcgc cgcgacgatg aggaggacgg
      661 cgtcggcccc cagcgagagg gcgaggtcga gctggacctc gtcgacgacg aagtccttca
      721 tcaggacggg gagccccgag acccgcttca cctccgggag ccaggcgagg tcgccgccga
      781 agaagtcggg ctcggtgacg accgagatcg cggcggcgcc ggcccggcgg taggccttcg
      841 cgacggaccg gatcgcgggc aggaggtcgt cgccggcgcc ggagagcccg gagctgcctc
      901 ccggcagcat cgcgcccgcc gacggcgagc ggtgcttcac ctcggcgatc acgcgcgggc
      961 cgccggcgcg gagcgccgcg aggaaccgtg cgccgtccga cggaagggac gcggtcccgc
     1021 gcggggcgag ctcgcccgcc gcgagccgct tccggcgggc ggcgaggatc tccgcgagga
     1081 tcgagggaac gctcacgcgg cctcctgcga gatcgccgcg agcctctcga gcgcccggcg
     1141 cgcggcgccc gagtcgatcg acgcggcggc gcgggccatc ccgtcgccga ggtcgagcgc
     1201 gacgccggag acgaggagcg ccgcggcggc gttcatcagg acggcgtcgc gccgcggccc
     1261 ctcctcgccg tcgaggatcg agcggaggat cccggcgttc gtttccgcgt tcccgcccgc
     1321 catctcgcgc gggtcgcgct cggaaaggcc gaacgtggcg gccgtccact cctcgagccg
     1381 gacctctcct tccgccacct cggccacgat cgtcaggccg tgcgggagga gctcgtcccc
     1441 cgcgcccgag ttcgagacga cgagcgcgtg cgccgcgccg agcgccgcga gggcgtgcgc
     1501 gaggaccg
//
LOCUS       DAOTEZ010000696         1506 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate AMR_MDS_3116
            NODE_80335_length_1506_cov_7.154376, whole genome shotgun sequence.
ACCESSION   DAOTEZ010000696 DAOTEZ010000000
VERSION     DAOTEZ010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868140
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (wastewater metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 14:08:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,139
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 3 of 31
            Pseudo Genes (incomplete)         :: 27 of 31
            Pseudo Genes (internal stop)      :: 3 of 31
            Pseudo Genes (multiple problems)  :: 2 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80335_length_1506_cov_7.154376"
                     /isolate="AMR_MDS_3116"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1506
                     /locus_tag="P5164_20910"
     CDS             <1..>1506
                     /locus_tag="P5164_20910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HRY46406.1"
                     /translation="KGMNAFRQDIAAALAAVDFEHDPEAVDRREQLKAMDVACEALVV
                     FAERHAALAEERAATEPDAARRAELRKIADVCRRVPAEPPRDFHEALQMYWFCHLGVI
                     TELNGWDAFSPGHLDQHLLPFYRRGLADGSLTPEGARELVECFFVKFNNHPAPPKVGV
                     TAAESGTYTDFANINLAGLLPDGSDGSNEVTHLLLDVVDEMHLLQPSSNVQVSRKTPE
                     PVLKHVLRVVRKGYGFPSIFNADAVVEEQLRQGKTLEDARAGGCSGCVEVGAFGKEAY
                     ILTGYFNLAKVLELALHDGVDPRTGVRLGPATGAASSIATFDDLYAAFAAQLRHLVDV
                     KVRGNQVIERLYATRMPAPFLSVLTDDCIAKGRDYNAGGARYNNTFIQAVGIGTVTDA
                     LAAIRRLVFEERGLSLESLVAALDADFEGREPLRQRLVHRMPKYGNDDDAADDLMVRV
                     FGTAFAAIDGRPSARGGTYRLEMLPTTCHVYFGEVCGATPDGRRAGKPLSEG"
BASE COUNT          229 a          566 c          507 g          204 t
ORIGIN      
        1 gaaggggatg aacgccttcc ggcaggacat cgccgccgcc ctcgccgccg tcgacttcga
       61 gcacgacccg gaggccgtcg acaggcgcga gcagctgaag gccatggacg tcgcctgcga
      121 ggcgctcgtc gtcttcgcgg agaggcacgc ggcgctcgcg gaagagagag ccgcgacgga
      181 gcccgacgcg gcgcgccgcg cggagctccg gaagatcgcc gacgtctgcc ggcgggtccc
      241 ggccgagccg ccgcgcgact tccacgaggc gctccagatg tactggttct gccacctcgg
      301 ggtcatcacc gagctgaacg ggtgggacgc cttcagcccg ggccatctcg accagcacct
      361 cctccccttc taccggcgag ggctcgcgga cgggtcgctc acgccggagg gcgcccgcga
      421 gctcgtcgag tgcttcttcg tgaagttcaa caaccacccc gcgccgccga aggtgggcgt
      481 gacggcggcc gagagcggga cgtacaccga cttcgcgaac atcaacctcg ccggcctgct
      541 ccccgacggc tcggacggct cgaacgaggt gacgcacctc ctcctcgacg tcgtcgacga
      601 gatgcacctc ctccagccct cctcgaacgt ccaggtctcg cggaagacgc ccgagccggt
      661 gctgaagcac gtcctgcgcg tcgtccggaa gggatacggc ttcccgtcga tcttcaacgc
      721 cgacgccgtc gtcgaggagc agctgcggca ggggaagacg ctcgaggacg cccgcgccgg
      781 gggctgcagc ggctgcgtgg aggtcggcgc cttcggcaag gaggcctaca tcctgaccgg
      841 ctacttcaac ctcgcgaagg tcctcgagct cgcgctccac gacggcgtcg atccgcggac
      901 cggcgtccgc ctcgggccgg caacgggcgc ggcctcctcg atcgcgacgt tcgacgacct
      961 ctacgccgcc ttcgcggctc agctgcgtca cctcgtcgac gtgaaggtcc gcggcaacca
     1021 ggtcatcgag cggctctacg cgacgcggat gccggcgccg ttcctctccg tcctgacgga
     1081 cgactgcatc gcgaaggggc gcgactacaa cgccggcggc gcccggtaca acaacacgtt
     1141 catccaggcc gtcggcatcg gcaccgtgac ggacgccctc gcggcgatcc gtcgcctcgt
     1201 cttcgaggag agggggctgt cgctcgaaag tctcgtggcg gccctcgacg ccgacttcga
     1261 gggacgcgag ccgctccggc agcggctcgt ccaccggatg ccgaagtacg gcaacgacga
     1321 cgacgcggcc gacgacctga tggtccgcgt cttcggaacc gccttcgccg cgatcgacgg
     1381 ccgcccctcg gcccgcggcg gcacctaccg cctcgagatg ctcccgacga cctgccacgt
     1441 ctacttcggc gaggtctgcg gcgcgacgcc cgacggccgc cgcgccggca agcccctctc
     1501 cgaggg
//
